LOCUS       DAOOZV010000001        85791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_306_length_85791_cov_65.202715, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000001 DAOOZV010000000
VERSION     DAOOZV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 85791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85791
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_306_length_85791_cov_65.202715"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLW08_00005"
     CDS             complement(<1..702)
                     /locus_tag="PLW08_00005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51021.1"
                     /translation="MKVLHIDAEKHWRGGQQQAVYLYEGMLKSDYKCGFVCCPGSQLR
                     QYFEKKRLPHHCLMFRGESDIISAFNLARLCRRYSYNILQLHSSHSLSWGLWAKFFYP
                     SLKLVATRRVDFPIGKNPLSAFKYKTKAVNKIVAVSDNIRKVLISCGVPSEKVVVIYS
                     GIDLNKFDTIQVPTDFRQTWNIPEKSIIVGTVAAFAGHKDYPNFLKAASLAVQENPNL
                     FFMAVGGGDLLSEMKN"
     gene            complement(699..1445)
                     /locus_tag="PLW08_00010"
     CDS             complement(699..1445)
                     /locus_tag="PLW08_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL51022.1"
                     /translation="MEQLSAVLIVKNEEQNIARCLKSISWVDEIVILDTGSSDATVNI
                     CKQFGAKIYYLDKWEGFGKAKQQAVDLATFDWILSIDADEEISSELQKELQELKTKRF
                     ENTAWRLKRRSYYLGKPIHFCGWQNDAPLRVFNRKQGKFNEKLVHEGVQTSQKVKSCK
                     HYLYHFTNPTAEVHYTKMRLYGELSTKQLYAQGKRANEFIAWVRGIFKFLKMFIFQLG
                     FLDGAKGFLLCKNSAWGVWYRYHLLGKMKK"
     gene            complement(1446..2597)
                     /locus_tag="PLW08_00015"
     CDS             complement(1446..2597)
                     /locus_tag="PLW08_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51023.1"
                     /translation="MNSLERVLGYPFYYTGVLADKILHWNNPSSLFFFFPHFHTGGAE
                     QVHLDILRIFADQKPFIIITKNSRNKTHYQAMQKSGRVIEIYKYLNNTNRFIVKNFFY
                     GFLASMINRHPKSVVISANSTIFYELSHYLTKSYLINILHGFYGINPKIWLLATPKIN
                     KRIVVSQAVYDEVQQYYKANGWEEKYNSRLKLIYNSVPLCDDISPKDYQAPLKIVFIA
                     RQAKEKRFYLYQQVARKIIREFPKIKFYCIGDHINTEPVINLGEICDRQKLYETIKDF
                     HILILCSISEGFGLVMAEAMTCGLVPLATDVGGVRESFQDGINGFLIKSKEEQEIINE
                     FISDIKVLNSSKKLLQQMSNSAREYVQEHFNYDRFKVEYTKVVEEGRNR"
     gene            complement(2594..3430)
                     /locus_tag="PLW08_00020"
     CDS             complement(2594..3430)
                     /locus_tag="PLW08_00020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51024.1"
                     /translation="MSISLIVPVFNYLNLLEKCLFSVYRQSVQPDEIILSDDGSDEDI
                     VAFYNAQKRKTAIPLILVRQKHLQFRAARVRNNGVSVSKGDVLVFLDQDIVIPQDYLK
                     CIMANLNQRKFLSGYPVRLSKEQSDKLTFEIIEQNNWNAILTAEQKRKIQKQYKKDFL
                     SYLLFRYLHLGGHGAKLRGGVSAIFKSDFELVNGYDENYIGWGNEDDDLGRRLQELGR
                     SGFNFVSNQIPLHLYHTHYHDQGERKNANYSERCKKKISHRNYRCQKGLSSIRNDLEI
                     LE"
     gene            complement(3427..4371)
                     /locus_tag="PLW08_00025"
     CDS             complement(3427..4371)
                     /locus_tag="PLW08_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51025.1"
                     /translation="MKISGFSFVRNGIKLYYPVIESILSILPICDEFVIAIGKSDPDD
                     DTKERILALKEPKIKIIDTEWDPKLCKRGVINAWQTDIAKNQCQGDWLFYLQADEVVH
                     EKYLPVIKKRCEELLNNKEVEGLLFAYKHFWGDYYHYQNGHGWYPFEIRIIRNDPHIH
                     SYQSAQSFRYFEYYDNPRQEKGTRKLKVAKVDAEIYHYGWVRPPQLMQNKRRALDSVH
                     WGKEKADAYYNNAPQYFDYGPLSLLAVFNGTHPKVMQERISCFDWQDKLQLTGKPNPY
                     REPHKHETFKYRFLTFIEKHFNGGKQIGTFKNYILLKR"
     gene            complement(4405..6321)
                     /locus_tag="PLW08_00030"
     CDS             complement(4405..6321)
                     /locus_tag="PLW08_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51026.1"
                     /translation="MAMKRKIKNRWNNIILIFRMMLRYWHYMLAGFISMLLFALFNGV
                     SVTLVIPLFDYVFKPNKINLLYTDFSSFLYALGEMLKQHISSSGGFFPAIENYSALWE
                     DAKVLMLQTDSLSLLYLLCIAVFLVILFKNVFYLLHKIFFNSVRGRTIRDIRNYMFSR
                     YLNQSLTFFSRHRIGDAIVRMVGDVEIVSEQLINSFVNVIREMITVLVFARIAYLLNP
                     VLLLYSIIILPVFSLTIGFLGKKIKKYSKRIQEQLSNLFSNVEEVLNSMKIVQAFRHE
                     QYEKKNFEAINNKHYHMWFKAQYYGTLSTPISELNTAITGIIVIIIGGKMILTPGSSF
                     SLGDFTAFLFALFSMLHPLKVITQVYTDIRKALVSLDRIGLVLNQESRIQDKPDAITK
                     ETFDSEITFENVTFGYKENKPVLKNFSLTIPKGSKVAFVGASGGGKTTIANLMNRLYD
                     VTYGAIKIDGIDIRDIKLDNLRKFFGVVTQDSILFSKSIKENIAYGSQEEVSDEQIIK
                     AAQIANAEEFINEMPNKYDEVLGSKGSDLSGGQKQRLCIARAIVGDPPILIFDEATSA
                     LDTDSEQKVQMAIDKATQNRTVILIAHRLSTILKADKIVVLEKGKIVGMGTHQELMQN
                     CPRYQHLYNLQQGK"
     gene            complement(6367..6443)
                     /locus_tag="PLW08_00035"
     tRNA            complement(6367..6443)
                     /locus_tag="PLW08_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6407..6409),aa:Arg,seq:acg)
     gene            complement(6967..7239)
                     /locus_tag="PLW08_00040"
     CDS             complement(6967..7239)
                     /locus_tag="PLW08_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51027.1"
                     /translation="MKFKKLIPTFIPFAYELIHIYKRNAKHDQNIRKKDRTAEKIGSL
                     EHLMVRLEKKVQQNREIYIKSFNKIKIWLAINSILLIAILVKLFIG"
     gene            complement(7248..8093)
                     /locus_tag="PLW08_00045"
     CDS             complement(7248..8093)
                     /locus_tag="PLW08_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51028.1"
                     /translation="MKKLLFVCLLIIPSVLLLAVFDDYQPSAMARGMGGAYTAVSKDA
                     NAIFFNPAGLINTTFSAKLSFSQLYNQDFSELKTVALGYKLPKNLGTVAIGARAMDVD
                     FEDVNLMSEQVYSLAHSFILLEDIHSRISFGYTGNLYLLSMDGEDDDTALGIDLGALA
                     VLHSRTHIAFAVTNINKATMGDENQIDLPSKLALGIAYVPYEKVTTSIEVKKDFAKET
                     EFMGGVEAHLLEPLAIRFGVHQNPATYNAGLSIYIQNLEVDYSFTYHSVLPGTHYFSL
                     GYKFK"
     gene            complement(8102..9535)
                     /locus_tag="PLW08_00050"
     CDS             complement(8102..9535)
                     /locus_tag="PLW08_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL51029.1"
                     /translation="MKNYILTFLAIFLVAGISAQTVLSCHDIQYTTDPTGNSPYYTQT
                     VTVQGIVTHIIPNSAFYIADPEGGEWSGLYVYHRNTSNVVSVGDLVKLTGTVDEYYNL
                     TEFTNVSAYEIISQGNPLPPFLELSTADMPSGTNHPENEKYEGVLVRFQDVTIKSTPD
                     TYGQFLIADHSNIWAMVDNGLYAIPATSIVVGESWYIIQGIVDFHSSAGFKLNPRNAS
                     DMIKQDTIENSIIKIVRTDPTTEPTLNSDVSMNLISTKIKSEWGVMSYSVTFKVNPTK
                     LIFSGLDIENTLTREMPEFHISAAGDTISWTYFSQDGIIYPDNDALLIKILVRPLVYG
                     ENIIDLVSFKYNGTEVNNLIDGSVIVKVPKKIAYLNIGKQGDKKNIFNPEMGEEIIIS
                     YGTKTGYLSKAVIRIYDAQGRLVYTPVHTNITSATGIQEFKWNGRDANLKLVPPGLYY
                     CHLEVTDRSSGEKDNTVQPIVIKSALK"
     gene            complement(9536..10102)
                     /gene="lspA"
                     /locus_tag="PLW08_00055"
     CDS             complement(9536..10102)
                     /gene="lspA"
                     /locus_tag="PLW08_00055"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPL51030.1"
                     /translation="MKQPRTQLKTAPAYFIMLLILVLDQLTKTLVRVNMEMYEHIPLL
                     QNLFGDTFILIRVQNSGAAFSFGFGSDLVNRIIFICVTVLVVGIIIYLLHYAQHKIQV
                     IAFGLILGGALGNLIDRVLFGPVTDFFSMDFPDFIMERFPVFNIADSSIFIGVVLMII
                     DMLFIKDKPSPLPETGEHIEISEFNKEL"
     gene            complement(10099..10500)
                     /locus_tag="PLW08_00060"
     CDS             complement(10099..10500)
                     /locus_tag="PLW08_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPL51031.1"
                     /translation="MATKKLPPEKLKHYEEVIRAELEESIAYIESINKEQSLGARESS
                     GDLSSYAFHQADQGSDTNLMEQSVMMLESEREKIRQLNEALQRISDGTYGICEFCGEF
                     IQENRLEIIPYATCCVACKEKNEDKKRRQKR"
     gene            complement(10561..11394)
                     /locus_tag="PLW08_00065"
     CDS             complement(10561..11394)
                     /locus_tag="PLW08_00065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPL51032.1"
                     /translation="MSSYKDTSAWLKTKLPKQPQIAVILGTGLSNMAEVFPLLYSIPY
                     NQIPGFVQSTAPTHKGNLLLCEVEGIPVIFLQGRFHYYEGYSMQEVVFPVRVLSALGI
                     KVLIVTNASGSLRKELEPGTIVQLQDHINFMGTNPLIGMNDDDLGERFPSMNKVYDPE
                     LCLLADKVAFDLGIKTAKGVYIAVTGPSLETKAECSAFASWGADLVGMSTVPEVITAR
                     HSGLKVLAYSIVTNYSNLFHNLAHSQEEIQRIADISGKQLQKLIAELILRLTRTFSLT
                     S"
     gene            complement(11395..11979)
                     /locus_tag="PLW08_00070"
     CDS             complement(11395..11979)
                     /locus_tag="PLW08_00070"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPL51033.1"
                     /translation="MPLFPLDIRHQEFSTAMFGYSKKEVRAFLDQIASEVEAFQKQLE
                     KEIARQEQKQNEVKQEALQAGSAVEELKRREELISRTLVFAEKTKADIIANARKEAEN
                     IIKEAELKAKRAIQEAKQYLSVLEHQYLQLKEQKRQFLMQFKVELQSFQDRINKDPLL
                     SKDSELRLDSEFHQIKEEIVPKPENTETQNEKNK"
     gene            complement(11993..12265)
                     /locus_tag="PLW08_00075"
     CDS             complement(11993..12265)
                     /locus_tag="PLW08_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPL51034.1"
                     /translation="MTYSTSLTGSILLFAVRIIQIYNILIIARVFASWIIRNPYNRLY
                     HFLISITEPVLGPIRHILPPMMGLDLSPIIAFFILDLLSRLLTSLI"
     gene            complement(12262..12825)
                     /locus_tag="PLW08_00080"
     CDS             complement(12262..12825)
                     /locus_tag="PLW08_00080"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPL51035.1"
                     /translation="MLNIVNQKTGWIEVICGSMFSGKTEELIRRIRRAEYAKQKVLVF
                     KPVIDNRYDANNIVSHSQMQAPSIPINAPGEIYSYLDDDVKIIAIDEAQFFDPSLVGI
                     CNDLADRGYRVIVAGLDQDYKGEPFGSMPQLMAIAEYITKNLAICVVCGNPANRTQRT
                     IHKDEQILVGSTDAYEARCRNCHEVLD"
     gene            complement(13386..13667)
                     /locus_tag="PLW08_00085"
     CDS             complement(13386..13667)
                     /locus_tag="PLW08_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HPL51036.1"
                     /translation="MKQLPKLLAVVLLLVIISSCTINKPICATSNPLGEKQGVYTQIG
                     ILFFQPVANSDAAIAKAAENGGITNISTVDYNTTWLLFLMKYQTIVTGN"
     gene            complement(13975..14712)
                     /locus_tag="PLW08_00090"
     CDS             complement(13975..14712)
                     /locus_tag="PLW08_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPL51037.1"
                     /translation="MRENVAVPKMSNEHLRYLSKLKQKKFRLQEGLVIVEGKRTLEQL
                     FAWGIKPLELYLVDGEPTVSAAISYTVSEQGMKRLCDSEQPQKIAGLFSLPKPRKIAF
                     KKAFYLDDISDPGNLGTIFRIAAAFNIDCLILSPKTCEISSPKVIRASLGAVYKVPFF
                     FCEVENLQELKAHIFALDMQGKIPLAHFAPPSEPYIVALGNEAKGISSSILKIAEETL
                     CIPMPGEMESLNVSISSAILAYTLSLN"
     gene            complement(14887..16563)
                     /locus_tag="PLW08_00095"
     CDS             complement(14887..16563)
                     /locus_tag="PLW08_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPL51038.1"
                     /translation="MKYFLIIFLLTIGIFLSAESWTVLLYMAADNNLAEMGRLDINSL
                     ESVAQPADLNLIVQADFPEGAKRYKIQQDNSEQITSPVLANLGTINSGDPNTLNSFIK
                     WGFNRYPAQRKMLIIWSHGDSWYKDNSGKWICPDDNAQDLISVSGGELVLALSGIPHL
                     DILLFDACSMQCIEVINEIHSFADYIIGSEDLVPQYGFPYEDIIPLFDGDFDELLAQI
                     PELYVSSYLPGQGINPGGALWSVTCSVVKTELVPQFVQQIKNFAISQRFLAPKYFTVR
                     NSCCSMNTGLADIDIRDFLEKAQTIWTSGTQNLLSLWTSIVISSCFTNPEEVGNIGTA
                     AIWFPDSRFNFENGWRQYHKLNFSRSRWLSFVNSAIGNDTFPPETPILISQNLIYNTL
                     QISLQANPDPDSLYYEITIDEGQHFQYFYTNPDNAVFTIMLQISQNGTYQIRAVDQSG
                     NKSQPLIGNYTYSNPQASIIINPNPVSGTSLAVLRWWASEELAGKIQLEIYNLKGQKV
                     INRYLGEVITGEGNFLLSAEPKFRALPSGVYILRLHLGNRKFTKKLTILY"
     gene            complement(16560..17639)
                     /gene="buk"
                     /locus_tag="PLW08_00100"
     CDS             complement(16560..17639)
                     /gene="buk"
                     /locus_tag="PLW08_00100"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPL51039.1"
                     /translation="MNYRVLAINPGSTSTKIAVYDDSTPVFEKTLRHDPEELNQFGDL
                     FNQTDFRKALVMEAMEENNVHPQSLNAVVGRGGLVRPVSGGTWKVNEAMLRDLKDITI
                     WGRSHASNLGAFLAKAVAEPLSIPYFIVDPVATDEMEDIARLAGIPEIEHKSLFHALN
                     IRYICRLLAAKLGKNLTEVNFIGVHMGGGISVAAIKNGRVVDVNNALLGTGPFSPQRA
                     GSLPIGDLIEMCYSGKYTKKELTTYLSKGAGLMAYLGTDSGIEVGKRVAEGDQKAKLV
                     LDAMCYRISKEIGSCSAVLAGKVDGIYLSGGLAYNDYIVNFIKEHTKFIAPIYIYPGE
                     KEMEALCQGGIRVLNGTEEAKEYPY"
     gene            17820..18179
                     /locus_tag="PLW08_00105"
     CDS             17820..18179
                     /locus_tag="PLW08_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPL51040.1"
                     /translation="MTPKEKNETQPADTHICEEECEHEQFVIPVQSEDGSTQNYIPIG
                     HIDYEDKQYIALVSEGEDTYDIYQMIGEGDIVTFYPIEDEELYDIIADKFNELFLSEM
                     DKMGLLDEENFLNPEED"
     gene            18396..18536
                     /locus_tag="PLW08_00110"
     CDS             18396..18536
                     /locus_tag="PLW08_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51041.1"
                     /translation="MNKELETDWQKFNPNNAIEEKWVGKFFLCQRMHRALKNKCVWFI
                     AN"
     gene            18880..21342
                     /locus_tag="PLW08_00115"
     CDS             18880..21342
                     /locus_tag="PLW08_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51042.1"
                     /translation="MKRKIVFWFICLFLCGGCWLTRKIDTHQFALKEMTQFATPLNGW
                     ITAGLKEEGEDFFICHSQYDENLYFVLVNDIQGKTTSQINLPSKIIDLNTLIDKRNND
                     QWLFISSNDSDSLYLMGVKYIWQIPLQRENKYFSSLERTDWAKGRVDYIYNGSIYPIF
                     LEDIDNDGKMDLLCQLIDFFTAKPRGLILYDFETGFIKWRYDLPCNPRSILWDDFDGN
                     GSKEIIVSNDALKNTTEIINDVDDCSGWIIVLSAKGNVLFKEKEFSGYGRIYLDAADV
                     NQDGIKEIYAVNCTWGSEKHFNIASVFNWNGKRLIKNRTLELPSTLESTQFPNFLQQM
                     DSRSDYYLHLVDTKAGLLILDTNLNPVKHNYKDFVNQIWDIGDLDRDGNKELFLQKKD
                     GSLEILDKNYYRRARIKNPFPDTIIYNANIVKTGFETKPLLVVGTNKQINYYEYTSLS
                     AFTILLSLLRNYALLLFILAALLCIILIIRNMYHKKNTLKLIDSWEQGILKVNKKGII
                     KHHNFKIKELANDFELLKRKKLEHCFPALAKPFQEFCNANLKTGSYIGTLNPNPQSKV
                     YQVYFIRMTSYRKFYYLVIRPYTEEGAIAEKMEWAETARRLSHHVRRYITNILLALET
                     LNTDTDPTRNKYYQIIRSEIDKVRVFTHAFQRFTELQDYQLELQDIIPSIEHCLSKIQ
                     FPENIQLIKNWSLASISAYIEPIRFEEALTNIVNNALEAMPEGGTLHISVKEFPLASS
                     PQGQLRVLIEIEDTGKGIPQKYLEDIWKPFFTTNQSGTGIGIPETKKIIDSMGGLINI
                     QSEENIGTVVTIWLKGDSNVRN"
     gene            21329..22678
                     /locus_tag="PLW08_00120"
     CDS             21329..22678
                     /locus_tag="PLW08_00120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL51043.1"
                     /translation="MLEISILLADDDLIFLQLTSDFLRKNGYAVDCASNIEATKTLLQ
                     KNNYDIMMLDMCFPALYDGFNLLDEVHENYPDLPVLMISGSGHIPDAVKAIQKGAADF
                     IEKPIDTTHLLLRLERIYDGIVTKKQMQSLRLSTIGMQGNSAIMQNIYDDICKAARFD
                     CPVLITGETGVGKELAARAVHRLSKYSAKNLVSINCASIPKELFEAELFGYESGSFTG
                     AVKAHRGYFEYADNNSLFMDEISQLPLEVQAKLLRVVSENEIQKIGGKVQKVNVRIIS
                     ATNQDLEKLTAEKLFRKDLLYRLNTIEINIPPLRKRIEDIPVLAEFFMNEFCTRNDIP
                     PKPISPSAVSWLCKQPWEGNVRELKNAVERAVVFSKNDHLTMVDFTTPSESDITDREY
                     GSLRKTITNFEKVYIENVLRIYNYNVRQAAYHLQMDKSNLFKKISALKITLPNHKPI"
     gene            complement(23052..23858)
                     /locus_tag="PLW08_00125"
     CDS             complement(23052..23858)
                     /locus_tag="PLW08_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPL51044.1"
                     /translation="MYKSIKQQQLEQRILKLLSGKALKTAEWLFANPELQAGQEYANI
                     VSIKRLGFNDHGPVHMRKATLNAIKMFNLLNRSGIKMNLEKEGAGTADDSLTAVILAS
                     LLHDLGMAITRESHELLSIQLAIPYIDTVLNTLHSENEIVLKTAIRSLAIEGIFGHMA
                     THKIHSLEAGLVLIGDGSDMEKGRARIPTMLSSQTRVGDIHRTSASAIQKVKIFKGQE
                     KPINITVEMNASVGFFQVEEVFFPKINISPVKPYIELYAGVTDREMLRYL"
     gene            complement(23871..24323)
                     /locus_tag="PLW08_00130"
     CDS             complement(23871..24323)
                     /locus_tag="PLW08_00130"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPL51045.1"
                     /translation="MRPFDTLTLMQFIWAFLAILGFSIRSNLKGIRILFTALSGGLCW
                     AFYLIILYYSKSMLFSVFVAIILVCIYSEIVAPRLKTPVSVFVTCAIIPLVPGRGLFN
                     SMQLYIAGDNLHASKAILQTLLIAGTISIAIAIVSSVTNLINKLMNRF"
     gene            complement(24323..25114)
                     /locus_tag="PLW08_00135"
     CDS             complement(24323..25114)
                     /locus_tag="PLW08_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPL51046.1"
                     /translation="MPKVSSQQVLELALEVGRIILQSGGTTNRVELMMKKVCNCFGYP
                     ETESYVTPTGIFISIKDENGNIFTSIKRIENRRIDLGKITRISTMVNCMEVKALSEQE
                     LQEQLVNFNSELKKIDNENPWSPWIRDLCGGVTSGFFCLLFGGSWAEFAVAFLIGVVV
                     TFGLKYLEKLAFNNFLLNAIAAALIVMLAKTLDIFLPYIRLDNIIIGGIMLLVPGLSI
                     TNAIRDTMSGDLVAGTARAVEALFITVGIVAGSASMLKIWSMWGY"
     gene            complement(25101..26711)
                     /locus_tag="PLW08_00140"
     CDS             complement(25101..26711)
                     /locus_tag="PLW08_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51047.1"
                     /translation="MQKNVKEALLLDKLFALLETNGEVMESVKHQKLNKKHYSFEELK
                     TQNRNTENLLKAIKESRALEARLESIFSELSLLPIKHLKTTERLELNELFEIKSFLFS
                     YLHLQEILQEHKLNHQHPLPDMQKMFSLLDPEGNKLPTFRIYPSYSSVLKKLTQKQFA
                     IAKRLKEARKRDLEKAKQELGMPTLKEEFTLSRNRKEQLEAILASKYFIVVSEGLANY
                     NFRLADSKETADLKAELHQLSIELTEEENKILCSLTTQIQNAFSNFELAYNSAGEYAW
                     DFCLAKFALHYNCCLPNITPSFEGIYLTEAVNLPLKLFLETLQRQYQPLNIAMSKKDN
                     LITGPNMGGKSTALITIGQMCILASLGIPLPAKKAKLPLYDEVYYNHDAGENSETLSS
                     FAREVVSLSNMLLRKGNKLVLLDEFAKGTNPEEGEAICVATLKYLINSNNTIISATHF
                     SAPAELAGLAHFTIKGIDEQGLKRLEKMQDSNLETRLALLSEAMDYSLLPVSEKTAPP
                     KCAIRIAGILGLPAEILQQLPKEKNAQS"
     gene            complement(26740..29049)
                     /locus_tag="PLW08_00145"
     CDS             complement(26740..29049)
                     /locus_tag="PLW08_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPL51048.1"
                     /translation="MEANRKAPAPFLLPVLFWTIGIILAKCFLPGIYFQLIVAGGLTI
                     VAIFMRKIRQILLLLLFLFLGMLRFSLCQQESSQLEILLQEKEHLQQEITFNVTRVFS
                     VEEKRYAIELDSLASYPLQEKIILSTNESLIPGRTYRLLAEIYPLTRDPILDIYPNRY
                     SAVAYQLGKAQEFPNLKVSAIIGRLRYELLSSLEAKLGEDADWAKALLFGESGLKQEY
                     ITQLTGAGIIHLIVVSGMHIWFIYLIMVSLLRMFFKREIAEFLFLPLILLYAALNNWS
                     PSVTRSIIMISVGILARWLQSPVSGAQTLALSLFIITLLSPLQLFNIGLQLSFVSVLV
                     IFYGLPNFRLFSRDKILNNPFYKVLNDLQDGILLSALISIAITPFTLYYFGTASLNGI
                     IGNTLGIPLSGILLPLSFLVLITPQSWYLTKIFVIVYRAVNILWIRWMQFCYNLPFSL
                     SANYLSLFQAIALAVIILWGFFLIRGKFKFALQAVIPMSLLVTGFMLIPTWQKPEASV
                     YIFNCGVGDCTLIRLANGETMLIDTGGTKKKGFAENILTKSDYSEESWLNKNLLPWLG
                     KKGITKIDYLVLTHLHSDHCGGLITLLNHKKIKHIFVSDESTKQELWNYASKQKYFGT
                     AQIHTVTDTCSFFLGKAKLKFLHPDKSYHPLNENNSSLVCRLDTKKERLLFTGDIEAE
                     AENYLVDNYAQELKADFLKIPHHGSRNSSSSEFLQAVNPQEVWITASSRNIYHFPHPE
                     TIKRLQKQQTKILITGNGTIRKMIDSRSN"
     gene            complement(29361..29774)
                     /locus_tag="PLW08_00150"
     CDS             complement(29361..29774)
                     /locus_tag="PLW08_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51049.1"
                     /translation="MRVVDRLEFPKRYKPKQFILIIVIVLVILGIFIQRSGVRRNASR
                     IQISDVRISNFGTQFIELEYTLANTGKRDQEIALMARVWDVEGEEIASALFSVKVKAN
                     SISKRTKMLDKLNRALQEGERPYKVEIALYTRHIP"
     gene            complement(29785..31311)
                     /locus_tag="PLW08_00155"
     CDS             complement(29785..31311)
                     /locus_tag="PLW08_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51050.1"
                     /translation="MAKKICLAILLLFCSLLPALRLIPDSAFAPDIQQGLKPSLLSYP
                     QRAKEDSISIYNNGLYLPFVSRSSVGDAVLDICRLGNTNYSILSYQSPDYKLRGELNF
                     IGGLEPVKKDESYSFLYRGWQLKSQYGKHIEMNTKWWNGIFLGNQNAARTSWLVDGYC
                     NTTDEKLNIDNLSGDISYNAKNLTVALGRGKFPVGNSISGSIILNDGVNDYGYILAEG
                     RAGIFSISFLHASLMADSTYNIYNDHFYNNKNYPDKYLALHQFGFHPCSNLHLFLGEE
                     IIYGNRSPEISYFLPNAFWRAIEHNLWDRDNVMIFAGGSYRPVQPLFLYAQVLIDEFS
                     SSKLFSDWWGNKYALQGGMRWNVPFSLPESPSADITTEITAVRPFTYTHYLNHTMFST
                     DGRPLGYPKGSNLVDVSCLLRLPYKDIFLWQGGVSYCKQGSFGSDWRQNYHNVFNTAE
                     EIDEGTADWFQGTKTETITLKSNVLIDIFAHHRLLFGFETSHLDDWQNTFYSAWQFIY
                     "
     gene            complement(31315..32364)
                     /locus_tag="PLW08_00160"
     CDS             complement(31315..32364)
                     /locus_tag="PLW08_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51051.1"
                     /translation="MTKRFLVTLILLTAVFCSALEVTYIDWQNYGSAATRIVIGLYGT
                     GDYKVDKSNDFVEIKLDNADVSKVDISNSAEGLIKSIEQFGDKIVISTFCPYCLEQME
                     LDAPRRLVLDLIVAQPDKQQKIELADFYTSVGKLNSADKIYSELHRNYPSDYEILYKW
                     ACLLKQRGSGRLKEVVGKIPEKSEYYSLAQQMLKGTEKKDSAQLCSPQKAQNACKISS
                     VEEKTTTDSLYIMPKTQQDKKQTTKPAKVKKLYGRKWISRIMLLIILMLLLILIYHFI
                     ASISRKKRKTTLNINIEPENKEDIHPEENNTLCLMVRRLLDFGWTNSEIAKELKISEE
                     EVERLVQLCNQDESQ"
     gene            33690..34703
                     /locus_tag="PLW08_00165"
     CDS             33690..34703
                     /locus_tag="PLW08_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL51052.1"
                     /translation="MIKSVVMQNFGPLVDTKWENLGKINVIIGANSSGKTFYLKALYT
                     AIKSLEGYKRGNENRSLAEILADKLYWTFQVPKIGDIVTKGQPDPLSFEFSVGNKDLS
                     YTFGKDTTKKILSLENYIYPQKSDSIFLPAKEVLSIYQNIIQYYDQYKTFGFDETYVD
                     LVRALSIPRIKGKTYQIIAKSRKKLGEIIQGKIKFDTDSQVWQYKKGNVRYPIGVTAE
                     GIKKIGILDTLLGNRYLTPQSTVFIDEPEAALHPSAVSLFLEIISDLAELGMQFFMAT
                     HSYFVVKKLYLIAQEKKLNIPIASYEDNKWVYDDLRNGMPDNSIVNETIKLYKEEVDL
                     VLK"
     gene            34700..35212
                     /locus_tag="PLW08_00170"
     CDS             34700..35212
                     /locus_tag="PLW08_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51053.1"
                     /translation="MNKIIIEESNMKFGPYDKEAVFLIEQSRVYKEIGNNVKIAEFVL
                     EKSSQNINIIEAKSSSPQPQNNEDYDTFIRSIYEKFVNTFSLTLALILKRFPSHYEVL
                     PERLEKIEVSNFKCLFVLVIYGHKEEWLLPIQDSLNKSLSSFFKIWNLPPTSVIVINE
                     KIAKAKHIIK"
     gene            35221..36246
                     /locus_tag="PLW08_00175"
     CDS             35221..36246
                     /locus_tag="PLW08_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPL51054.1"
                     /translation="MNTDYLNLAKAFLYKQRLWQTCGIVCEYGLSLITHKPLVHFYPP
                     ALMLEPTNICNLHCPLCLTGNGKLLRPRGMMSLPLFKKICREVQNKIGMLILWSQGEP
                     FLNPDFYAMLEYASKQNFYTMTSTNASLELDIERIVKSGLHKIIISMDGITAETYNKY
                     RINGNFDLVLKNMQELVHQKEKLHSNKPYIVWQFLIMKHNENEIEEVKHLARKLKIDK
                     LEFKTAQIYSSDDLAFLPSDHKYSRYFQQGNKFTLKTKLKNRCIRLWTQPVINWDGEM
                     NICCYDKNGSIKIGNIQEHSFSELWCGEKMQKMRKAILNNRSAFEICQNCGEGIVQKI
                     KMEKGER"
     gene            36722..37807
                     /locus_tag="PLW08_00180"
     CDS             36722..37807
                     /locus_tag="PLW08_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPL51055.1"
                     /translation="MKISEIMKQIEEFAPLGLALSWDNSGLQIGDLNSEVENVYITLD
                     VTSTAVQKALENECQLILSHHPLIFQQLKNITNPLYLEIIKNNLSVISLHTNLDVAEK
                     SVNHSLAQKLGLEVIGFLSSETGATNYIIVVYCPLEDAEKIMHSAWSAGAGKIGNYYN
                     CANKQESEGYYKASENAKPYKDSGIEGHIAETCLQFCCESFHLNDVLNAIRQNHPYET
                     PLIYHYEVANPNPAYGLGLICKYNAEINLQDLAILVKERLNCPQVKLWTASFDLHKNI
                     NRIAICGGSGSSLISAAQNSADVLITGDISYHSFLESKIPLIDAGHFYTEYPALQLLA
                     DFVNKLQLHPFLIPQNEHLYTQNLLII"
     gene            37838..38905
                     /gene="ispG"
                     /locus_tag="PLW08_00185"
                     /gene_synonym="gcpE"
     CDS             37838..38905
                     /gene="ispG"
                     /locus_tag="PLW08_00185"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPL51056.1"
                     /translation="MQEIKRKNTRRIQLGNIPIGGGAPVSIQSMLSVKTSDRENAIQQ
                     IQELESVGCDIIRCAVMDLDDAKAIKDLKKIAQAPLVADIHFNYRLALAAMENGIDGL
                     RINPGNIGKIEGVKALVASAKERKVPIRIGVNSGSLPKDLLQKYGVSAKAMVESALRH
                     IRILEELSYYEIKVSVKASSIPLTLESYRLLSSVCDYPLHLGITEAGTVFRGSIKSSI
                     ALGILLDEGIGDTIRVSLTDKPVQEVKVAKEILTSLGLRKGLNIISCPTCGRTRIDLI
                     KLANEVEKALEPYNNYPLTIAVMGCAVNGPGEAKEADFGIAGGIKEGLLFAEGKVLKK
                     VPEAFLVNELVALVQDYISKH"
     gene            38908..39441
                     /locus_tag="PLW08_00190"
     CDS             38908..39441
                     /locus_tag="PLW08_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPL51057.1"
                     /translation="MLLKIFFTFFKIGAFTIGGAYAMIPLIKREVCQKNNWLTEDEFW
                     DGLSAAQSCPGPIAINLSIYIGYHLAKGKGMAIAVLATILPSFIIILIIAMLFQQFAE
                     ITLIQKAFHSLRPAVVALIAVPLIQMSRQSGLALSNFWFPLSVAILVGFFRISPVYLI
                     LITIAFAVYQGLKKQKL"
     gene            39438..39956
                     /locus_tag="PLW08_00195"
     CDS             39438..39956
                     /locus_tag="PLW08_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPL51058.1"
                     /translation="MIYFQLFLTFFKIGLFSFGGGYAILAMINQEVVLKNAWITHSQF
                     MDIVAISQMTPGPIAINAATFIGYQKGGFVGSVLSTLGVISPSLIIMTFITLTYIKLK
                     NQPWFKNIFQKLRLLSLGLIAAAFIMVAGDAFRDLFSVLVFTASFIASWKFKLNPFYL
                     LIGAAIVGIAFG"
     gene            complement(40071..42524)
                     /locus_tag="PLW08_00200"
     CDS             complement(40071..42524)
                     /locus_tag="PLW08_00200"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPL51059.1"
                     /translation="MNKAFCEKLSHLSIEGTAEEIVHRFIEHLKFDLGKDEFSATPYD
                     CYVSFASAVKDILMDRWLITQPQEYALQRKRVYYLSLEYLIGRSLGNAILNLDIQKQS
                     IKALTENGFDLSLLSELEWDAGLGNGGLGRLAACFLDSMATLKIPAYGYGIRYEYGIF
                     FQHIINGEQVETPDNWLRYGSVWEIARPARLFPVYFGGIVKEEILEDGSKKMRWIPEE
                     TVMAMAYDYLVPGFQNNYVNTLRLWSAKSTREFNLAYFNEGDYMQAVADKNHSEMIAK
                     VLYPNDNKMQGKELRFRQEYFFVSATLQDILRRFLKKESDLRKLPEKAAIQLNDTHPA
                     IAVAELMRILVDERNLPWEIAWEITQKCIAFTNHTILPEALEKWQVLLFEKLLPRHLQ
                     IIYEINHCFLSSLRATGLYDDDFISRVSLIEEEPVKSIRMANLAIVGSHSVNGVSELH
                     TEILKHLIFKDFYALWQDKFNSKTNGITPRRWLMLCNPRLSELITQAIGSAWKTDLKE
                     LAKLNKYKNDSTFLEDLGEVKRQNKLEFCKYYEDLHKRKLNPESIFDFQAKRIHEYKR
                     QHLNALGIIHLINQIRDGKTPYPQSFFFAGKAAPGYFIAKLIIRFICALSDYIEKDKE
                     LSRYLSVIFLPNYRVTLAERIMPPAEISRQISLAGTEASGTGNMKFALNGALTIGTLD
                     GANIEIREAVGEENFFLFGMTAAEVKNLKESGYHPYEMMRQNEDLKRIFDFIESDILN
                     PLNPGLFTPLTDLLLYQGDKYCLIADLPDFIRVNTEAVKVYQDKQQWNRMSLANIANM
                     GKFSSDETIKGYAKDIWGV"
     gene            42994..43140
                     /locus_tag="PLW08_00205"
     CDS             42994..43140
                     /locus_tag="PLW08_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51060.1"
                     /translation="MQENFPCGEYKIIWDGLNDHGTTVSSGVYFAEMSTPGYRKQIKL
                     LLTK"
     gene            43161..>44488
                     /locus_tag="PLW08_00210"
     CDS             43161..>44488
                     /locus_tag="PLW08_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51061.1"
                     /translation="MKIRILILSVCILMLSSVLLAQEMVPLSNLKEQKIEFKSVPLSS
                     NRTVPQFTFTRTPISLLTSYYDYMIGGYNGMPVQVIPDNVTGGGGYFLTYHARRTATG
                     TRRIFYAYLNADMDVIFNIEITNVTNNEGYPTVAVDPVSGKPFYAWHCNTDADTQLEV
                     QLTSDAFYEGIFGLFNDYITLIDNPITVSGTTDNEFIWPTAVIGPSPFAGKRRIYVVA
                     KNSVTHSYGPSENPRIAWADFNGNDIEMGYPLLWNYTSIPELDQWNIDIQWRRPFFAI
                     TCDNIGNLYYAGFHFAKEADGSTDILEPDLDVFKCDNYGTGTWVHLSKWSNLPSWNPD
                     ISPTNNNGYFTDTNNIPYEDNELFWKVMAANSGHLNAIVDDLGRIHYPALWGLNNADG
                     YFYPAMQYPKEVIFDPSQPTDSQFRINEIWPQKNPANTFDEYYQPWDNEPP"
     assembly_gap    44489..44588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <44589..46494
                     /locus_tag="PLW08_00215"
     CDS             <44589..46494
                     /locus_tag="PLW08_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51062.1"
                     /translation="NNMKITEGNGHGMLAVVWQNCRRAKQYNANGDTDYSAWANTPEI
                     WISVSSDNGDHWSEPIKLNNIETPEFAGLKPMWVYPANKVKYVGMQGNQKIGKLGIMF
                     YNDFTWGAYVISPGVGSTNDGGQVMFTELQIVFPPEGQAQDPFGQPIILSGSMSVMAG
                     VHINGQMASAGDVVAAYVNVNGSPQLRGKETVQINDNIAGCLLQVYTETNGETVYFKV
                     WDASTNQVYNVTETLSSQVNGVIGSWPDNLFWLNATLTQQQNIPLNTGWNMVSLNVHP
                     NSMAITDIFANIMANIVMIKSPDGVFIPGNPYNTLTQLADGKGIFVKVSNPCVLSVTG
                     MPIDITAPIYLNAGWSLIGYILPIPLNISSALISLTGNYIQVKGVEGVFEPYNPFSTL
                     SVLSPGRAYWIKLQNPGVLFYAYPLKEEQSPQNILSCTKWGTPMQKTNSQAVLCSLGS
                     FASEGDQLAALVQGELRGLAPLIKHNSILGTYINIFTDSPGETIEFKLIRKNSGEIID
                     LQPNLSSEPGETTGDYGNGIFYTLTQAPNQIPEIVTELVSAYPNPFSVSTSITLNVGK
                     EKQPVQIDIYNLRGQKITTLLQDKLEFGQHNIVWNGTDENGRRVASGVYLCKMHSENK
                     TQSIKLMILK"
     gene            46509..48065
                     /locus_tag="PLW08_00220"
     CDS             46509..48065
                     /locus_tag="PLW08_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPL51063.1"
                     /translation="MIHKIIPLLLLTLFAGAALAIDPWGEPEILPGSMTVMAQVSISG
                     SPAVNNDVLAAFVNVNGTLQLRGKAFIQVFGGVSGCLIQIYTENNGEIITFKVWDESI
                     QTVFEANQTLVSEVNSIVGSYPDNMYQIEAGQTTVTDPWLEPVILTSSMTVMAKIGIS
                     GVPATNEDLLAAFVTVDGIEQLRGKGNISVINGIAGCLLQIYTEVNGETIYFKVWDYS
                     AQQIVNAFPTLNSEVNGIVGSWPNNLFHIYAGNVQTVATPAFLPLGGTYQTAQNVTLT
                     CATSGAQIRYTLNGTDPTETSALYTNPIHLNLNSTTEIRAKAFKEYWIPSNIVSNTYI
                     ITGTVPAPSFNPPAGSYLNAIDVIISCALEETVIRYTLDETEPNENSMLFTAPIHINQ
                     DTMLKAKAYKTDWLPSNVVSAIYQISSANEDNLNAPLVTGIQNVYPNPFTGCTTIEIA
                     IKEYPQNYLLKIFNIKGEQVRQFNGSAKGIITQNWDGTDNKGNKLAAGVYLLYFQVGS
                     NHYTRKLILQ"
     gene            48764..48946
                     /locus_tag="PLW08_00225"
     CDS             48764..48946
                     /locus_tag="PLW08_00225"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPL51064.1"
                     /translation="MKIDKNLDIWKKGRQLATNVYELAKELPEEEKFVLSEEITQTAI
                     LMISEIPNSSTPALQN"
     gene            48962..50311
                     /gene="der"
                     /locus_tag="PLW08_00230"
     CDS             48962..50311
                     /gene="der"
                     /locus_tag="PLW08_00230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPL51065.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRSAIVDFEAGITRDRKYE
                     DVEWNGKIFKLVDTGGIVFNSIETIDKMVLHQVMLAIDESDLIIFMVDAQTGTTDIDK
                     EIAKILYPHKDKVMLVANKADNEKYEWEVYDFLQLGFGDVIPISAAQGRNTGDFLDAV
                     IERIPGTQSIFQLEEKSSATRIAVVGKPNVGKSSIVNLLLGNPKLIVTEIPGTTRDAI
                     DSPFRYHNKDYVLIDTAGLRRKTRVNYGVEYFSTLRTIEAIDRCDIVVLVLTADEELS
                     VQDIKIASYAKRKMKEIMVVYNKWDLIEKETNTINKYLKELHYQMPFLQFAPVLFISA
                     KTTQRIHRIMETVAKIEEESEKRISTAELNRFMEKVIMHRPPTHPSGKHIKIYYITQA
                     AVKPPTFIFFCNTPSLITENYRRYLHNQLREMFKFEGVSIKLIFKGRKKGEDKLELQ"
     gene            50286..50930
                     /gene="plsY"
                     /locus_tag="PLW08_00235"
     CDS             50286..50930
                     /gene="plsY"
                     /locus_tag="PLW08_00235"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPL51066.1"
                     /translation="MRINLSFNNFLPYIILLFAYLLGSIPIGLIVGKVFYHKDIRKEG
                     SGNIGATNALRAFGTSTGLIVLLLDMAKGFIPVMLATSLLEKGSPFIALTALLVILGH
                     IFPLYLGFKGGKGVATASGAFLALTPLSLLIALITFIIVVAIFRYVSLASICAAFVFQ
                     WHCFALLLKEAKPDYATVILVTIVVLMIISKHKSNLERLLNKTEPKLKFTKKGN"
     gene            50940..52322
                     /gene="purF"
                     /locus_tag="PLW08_00240"
     CDS             50940..52322
                     /gene="purF"
                     /locus_tag="PLW08_00240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPL51067.1"
                     /translation="MCGIIGVFGNDNAGRLAAVGLFAEQHRGQESCGMAVSDGLLIRL
                     HKKMGLVKEVFHEEQLNALPGKIAIGHVRYPTKGSATEFNTQPHLVETLSGPCYALAS
                     NGDIVNYAQVRKMLENEKVYFKSDNDAELLVKYIAYRILHYKENIGDAIRNLMRDIKG
                     AYSSVLCTPTELYMFRDPYSIRPMIWGKTPEGTVIVASESCALDTLGVQDRKEVPPAG
                     IIKVNDSKIEVLDNDPNLYREGNCEKHCIFEQIYFSRPDSFHFGENVYAVREKIGAAL
                     ALQDEGLNPDLVVPVPDSSNFIGLGYANQNGIPLSLGLIRNHYIGRTFIKPEQAVRDE
                     SVRQKFNVLPNFFTGKRIVLIDDSIVRGTTIRKIVRLIKDAGAAEIHLRIGSPQIKHS
                     CYYGIDTPTTEELIANRRTLEEIREITGVDSLKHLPLTDLKTCVHCPENYCYACFDGN
                     YPVGVPDVYN"
     gene            52463..53455
                     /gene="rfaE1"
                     /locus_tag="PLW08_00245"
     CDS             52463..53455
                     /gene="rfaE1"
                     /locus_tag="PLW08_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPL51068.1"
                     /translation="MYSIKPEKLEEILKSFQNRRIIVLGDIMLDEYLWGKVQRISPEA
                     PVPIIEVNSTDYRLGGAANVAMNLSVLNAKVDLVGVCGKDTQAEIIVQQLKKREMPVE
                     GIFFDPSRPTTLKTRIGSVSQQIVRLDREEVAEIDNSIQKEIFNYLVKIIPLCDALLI
                     EDYNKGLLSFNLITDVLQLALKHKKMVAVDPKYSNFSSYGGVEIFKPNYRELESYMGR
                     KLTDQDDFERSTEELRQRMSIKHLVVTKGSEGMYLFSENKPVNHLPSFAREVFDVSGA
                     GDTVISALTLAYLYDRNIETAALIANHSAAVAVAKKGTASVSIEEIWDSFNAPK"
     gene            53442..53921
                     /gene="rfaE2"
                     /locus_tag="PLW08_00250"
     CDS             53442..53921
                     /gene="rfaE2"
                     /locus_tag="PLW08_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPL51069.1"
                     /translation="MLQNKIIPYSEIAELSQKLHQQGKKIVFTNGCFDILHCGHILYL
                     EKAKALGDILILGLNSDASVKRLKGNNRPIVSEKERALVVAGLESVDYVCIFEQDTPY
                     ELIDLIQPDVLVKGGDWTIDKIVGTDIVQKKGGKVLSLNYEQGFSTTNIIERIKQDK"
     gene            53923..54333
                     /locus_tag="PLW08_00255"
     CDS             53923..54333
                     /locus_tag="PLW08_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPL51070.1"
                     /translation="MQEEIIEDSGIVKKVEGNSIIVEIERGGGCNSCKMQGFCFKKNE
                     PAEFEIVTDIPLQVNDRVQLDISPLGRTLVSLLIFGVPLLFLFLGFLIASLWFTEIVS
                     ILIGFTAMALSFRIIRYIDTKYGKRLKISIARKL"
     gene            54330..54713
                     /gene="folB"
                     /locus_tag="PLW08_00260"
     CDS             54330..54713
                     /gene="folB"
                     /locus_tag="PLW08_00260"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPL51071.1"
                     /translation="MKIRLNEMVFYGFHGVNAEERSLGQRFIVSVVLHTNPTIDKTIR
                     RLEDTIDYTKIYAEIKEIMESRQFYLLEDCANTIADKLLADFPLLEKLTICIQKPSVP
                     IPGSLKSVEVILSRTRNNQEDNLSQ"
     gene            54710..55213
                     /gene="folK"
                     /locus_tag="PLW08_00265"
     CDS             54710..55213
                     /gene="folK"
                     /locus_tag="PLW08_00265"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPL51072.1"
                     /translation="MIYYLCLGSNLNNPVSQLDGAIQHLENDPGIKILRSSKRIITEP
                     YGIINQPDFLNQVLEVESSYQPQELMERLLNIEKAMGRIRRIKWGPRNIDIDILLAGD
                     LVLDSRITPLEKSSPEVIIPHPDLHNREFALRLLNELIPNAKHPLMHKTIYELYYSIV
                     AEEEYYE"
     gene            55206..56234
                     /locus_tag="PLW08_00270"
     CDS             55206..56234
                     /locus_tag="PLW08_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPL51073.1"
                     /translation="MNNLTGIILAAGKGTRMKSERAKVTLPLADKPMIQRVVETALSA
                     NCQKIYIVVGYMKNNVISAVEDNEKIEFVEQEEQLGTGHAVMITEPLFTNPDQDVLIL
                     CGDVPLLSAQTLIRIYEKHKSSSAACTVLTAFLDDPGKYGRILRDTSGKICGIKEYKD
                     ASEEQRKIKEWNTGIYCFQVKNLFSALKQTSNHNQQSEYYLTDTIEILYRQGKTISNV
                     VLEDLMEVSGVNSQEELAALEDIYVDRIRKKWLNNGVVIHNPHSVYIGDEVIIEPDVE
                     IHQNTVIKGKSTLEKGCVIGPSCYLEHSTVSLDSVLQGHNILVNAILPEHHILDFGSH
                     IIEEIRYE"
     gene            56227..56748
                     /locus_tag="PLW08_00275"
     CDS             56227..56748
                     /locus_tag="PLW08_00275"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPL51074.1"
                     /translation="MNNNKKSKDEHHYLYSPWRLDYILGEKDGDCIMCRFQNTDNDKE
                     NLILHRAENCYVMLNRYPYNNGHLMIIPYQHCGALTELQEEAWLEMAKLVRNTEAILK
                     KVYNCDGINIGINLGSAAGAGIAEHLHIHIVPRWVGDSNFMSVIGGERVIPESFDSVY
                     NKLAPEFANLIKN"
     gene            56760..57209
                     /locus_tag="PLW08_00280"
     CDS             56760..57209
                     /locus_tag="PLW08_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPL51075.1"
                     /translation="MNLTNNPPENKLTTKRKIWLPITLVLIAIVAIVLFFIWKKNTEE
                     SKPVYSEEQLPAIKVVVTNGCGYERLAADFAAALKDKNIEVVGLSETPKPIYDKTIIV
                     IRKGDKEDLERLMKMTGIQRWTSAYSEYFSADFEIIVGRDYEQFIAY"
     gene            57219..57575
                     /gene="rsfS"
                     /locus_tag="PLW08_00285"
     CDS             57219..57575
                     /gene="rsfS"
                     /locus_tag="PLW08_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPL51076.1"
                     /translation="MLAEYISLEVIISWLKEKKAENIRIYDVQEKCDYTDIVVVCEGS
                     ADVHNKAIANYMVEMAKKNNLRILSKEGIDYGHWVLLDIGDLIVHIFLPETRDYYNID
                     ELLAKIAKRPVQENER"
     gene            57572..59221
                     /gene="argS"
                     /locus_tag="PLW08_00290"
     CDS             57572..59221
                     /gene="argS"
                     /locus_tag="PLW08_00290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPL51077.1"
                     /translation="MIKELLHKNIVEALDNLKLNHEKEFTVEIPNNPEFGDYSTNAAL
                     VLGKENKMKPKDLAEKIVKELKKNKAYKKLEIAGPGFINFFLSPSLYQQVFWEIYKSE
                     DYGKSDFGNQEKILLEFVSANPTGPLNIVSARAAAFGDTLYRIMKYVGYAPAREFYIN
                     DAGNQVDILAESLELRLREIHGEKIGEFPYEAYHGEYVKHLAHRLNALEGTRVFMLPQ
                     KDRLEHLKEFALSELLEMQRNSLEKFGVFFEGWVSEKALRAEGVVEEVLSYLTEADCT
                     YEKEDAIWFNSTKYGDDKDRVLMKSDGSITYFVPDVAYHLTKIQRGFTKLIDIFGPDH
                     HGYVPRIRAAFRALNYDDSILEFIFLQQVNIFESGERVKMSKRAGKIVTMDDLISVVG
                     KDAARYFFIARKANAHLNFDLELALKKNNENPVYYCQYAHARICSIIKKARSEKIYPK
                     HFKKEMIKKLNKPEELALIQKMADLPELLILIAVHREPHRLTTYIEELASLLHHYYEK
                     YQIVNVKNPALSQARLMLLYTVRKVMQICFELMGISAPETM"
     gene            59239..59691
                     /gene="rimI"
                     /locus_tag="PLW08_00295"
     CDS             59239..59691
                     /gene="rimI"
                     /locus_tag="PLW08_00295"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPL51078.1"
                     /translation="MDNAYSIRLSCEDDFDAIEEIEHQVYSNPWPPEAFSRMLFPWAF
                     TLLYGKEVIGYIFYCGVKEEMVIVNFAVKPDFQGKGLGEYLLTETMRMMNNNGVQRFY
                     LDVRISNYKARRLYEKVGFQEIGIRKQYYNNPDEDALVMGMEFNDIRV"
     gene            59675..61276
                     /gene="nadB"
                     /locus_tag="PLW08_00300"
     CDS             59675..61276
                     /gene="nadB"
                     /locus_tag="PLW08_00300"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPL51079.1"
                     /translation="MTYEYDYLVIGSGIAGLIYALQVSKYGKVAVVTKSSLNDCNTDH
                     AQGGIAAAIDSLDSFEAHIEDTYQAGAGLGKRQVITEVISSGPKLIQYLIDLGTDFTR
                     RDPNYDICLENLSLTMEGGHTRRRIAYAADSTGHQIMETLIAQCQKNPNIEIFEYYIA
                     IDLITQHHVVQDEGFIPGISCWGAYVLNINENRVDIFKARKTMMATGGAAQIYSPDTN
                     PKVATGDGMAMARLAGARLANMEFVQFHPTAFWSPDGDTFLISESLRGEGAVLRLSDG
                     STFMENYHPQGNLAPRDIVSRAIDSELKKRGEKFCYLDATIVPAEQLLRHFPSINNML
                     KARGIDFTKEPIPVAPAAHYFCGGVLSTIEGITDIRNLFVAGEVACSGLHGANRLASN
                     SLLEALVMAYKAGNHPSNLEEVQFPCIPEWKVIDEFNENEWVVISHNREIIGTIMQGY
                     AGIRRSRRLLKYALSRLENIYNEINNFYQHNAVRKEVVETRNMAIIAIAVVKSALMRK
                     ESRGAHFLVDNPERDDEHYKHDTII"
     assembly_gap    61406..61505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <61506..62131
                     /gene="tmk"
                     /locus_tag="PLW08_00305"
     CDS             <61506..62131
                     /gene="tmk"
                     /locus_tag="PLW08_00305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPL51080.1"
                     /translation="SGKGLFITFEGIEGSGKTTQIIMLVEVLKAKGIPYVTTREPGGT
                     KISEAIRDLLLNPEFMEMKPETELLLYCASRAQHTAELILPALEEGKIVISDRYFDST
                     YAYQGAARELDEEVINVLTRFSTYGRNPDLTFLLDLPVEVGLARIKNRNLDRLEQETL
                     SFHEKVRRQFLSIANKYPSRYIVLDGESQPEIIHRQILSTLQPFIGE"
     gene            62133..63734
                     /locus_tag="PLW08_00310"
     CDS             62133..63734
                     /locus_tag="PLW08_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL51081.1"
                     /translation="MNRPKQKLALISIISIWVLSALLLIVGTNAYAQNRPQSTNLYSQ
                     VQLFSEVLYKLKQYYVTDLNDEELIKAAIDGMLSSTDPHTTYFTPEEFKEFTTTTKGS
                     FGGLGIQIDKIGNYITVVSPIEGTPAYRMGITAGDRIIKVDDVSIVGFSTDEAVKRMR
                     GDVGTKVKITISRPGLPEPIDFELTRETIKIKSIPYSFKLDNGVGYIRITQFSENTLT
                     ELRAALDNLEKQNVKGLIIDLRWNPGGLLEQAVDTVNEFIGKNRLVVETKGRTENKQL
                     YTRFDVKERNYPIIVLVNEASASASEIFAGSLQDWDKGLVMGKNTFGKGSVQQLIPLS
                     NGNGIKITTAYYYIKSGRCIHKMSNDNILKGKEVSEEDLKKESETNLQQVYYTANNRK
                     VYGGGGIQPDITVESDFLTLFGVELRRKNVFFNFAVDYLVQHNHQFDINTEIDNKIMN
                     EFLTYVSKNDIKYTQADVDSANSFIRTSIKSELVRKVHGDEEAYKITISQDKQLMEAI
                     SLFDRFRTLEQMFTYAASLNEKKEK"
     gene            complement(63976..64740)
                     /gene="fetB"
                     /locus_tag="PLW08_00315"
     CDS             complement(63976..64740)
                     /gene="fetB"
                     /locus_tag="PLW08_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HPL51082.1"
                     /translation="MDISIYRLMLAALLLIFPLLIFSNLKLKLSGQLFNSFARMIVQL
                     AIIGLILQFIFNRENPWLAFLWMLIMLANAVLTLKGRLKFQKKILLPVLIFSLLTTTL
                     IVMPWLIIVVLRPEPLFAPRFLIPIYGMILGNSMNNCSLALERFESGLSENWKAYYTR
                     LSLGASQWEAILPAFRKAMQAALMPELLTIASMGLVTLPGMMTGQILGGASPLVAIKY
                     QMMIMIGIFSGVTITDYTAINIYLRKRFDKFYLPKP"
     gene            complement(64745..65404)
                     /locus_tag="PLW08_00320"
     CDS             complement(64745..65404)
                     /locus_tag="PLW08_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL51083.1"
                     /translation="MSEILSINIKRLFYGDQVLLNDIQLKVEKGERILITGITGSGKS
                     SLLSTLNLMNRNYEGEILFEGKNIVEYQPDILRSKICMVLQEPYLEDANVQEVLDLPL
                     HYSSLKHRRLPDRQERIKNLFTAFQLSYEYLQKPTSQLSVGEKQRIALIRVLQLQPDI
                     LLLDEITSALDQKTSGIISECIFNNFAGTVIAISHDPLWQNLWQRNWVLEYGTIIDRR
                     I"
     gene            65992..67134
                     /locus_tag="PLW08_00325"
     CDS             65992..67134
                     /locus_tag="PLW08_00325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51084.1"
                     /translation="MVNYKEIKVEFDYPINNYTMSILNALEKKYGESNIHFDYNDNQV
                     LVYLNNIDFEMFKSDIEELSLNNNNNDLNKISNAISNIKSYGIKGKKRLYVGYGDERK
                     TVNRKEKEKKREIFYYTRGNDFSEINNLLPDKYMDQIVCGDSKIILSQLPNNCIDLIF
                     TSPPYNFGLDYEENEDDQHWEKYFETLFAIFDECIRVLKYGGRFIINVQPLFSDYIPT
                     HHIISNYLMQKKLIWKGEILWEKNNYNCKYTAWGSWKSPSNPYLKYTWEFIEIFCKGT
                     LKKIGDDKKIDISGDEFKKWVYAKWSIAPERRMKEFNHPAMFPEELATKVIKLFSYQE
                     DIVLDPFNGVGTTTLVAKLNKRHYLGIDISSEYCSKAEERLSSFLF"
     gene            67233..67940
                     /locus_tag="PLW08_00330"
     CDS             67233..67940
                     /locus_tag="PLW08_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPL51085.1"
                     /translation="MKIPTVEDLINIYDAIIKGIDKEALNSKDRAYGGIIRAGKGSLV
                     ENIAKGIIQIAWDRLNGDQTHLSLNKKKVRIPIKVDYVKRIKNPEIKSYIQDHFGNYY
                     YELNIDVPVYIDNELVLGVECKSYTENAMLKRILVDFTLLKTQYPTLDCVLLQLESQL
                     NGDYSNISQSIIFGSSSTHTLLSYFDVDLYIITLLQGERKVKKPIHKKEYFKELTSES
                     IEKAISTFQNILSKYKK"
     gene            complement(68464..69360)
                     /locus_tag="PLW08_00335"
     CDS             complement(68464..69360)
                     /locus_tag="PLW08_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPL51086.1"
                     /translation="MDMKTFGYLYYKTRGYSPEDIIKQAGISEEDFEFFEKNLQDVLK
                     EIEMLRPVAENIGQDFTRLTRYIYERESDQTLGVPRPDAIKVRSGEIVPLPAIGTLKM
                     PDMPLAKAIEQRRSVRKYSAIPLQQEELSFLLWASSWARDFRSSEQMEITFRNVPSAG
                     SRHPLETFLDIRRVEGIKPGLYYYHPVKHCLVLYDDSPEIAAKIHEGCMFQEMIPKAA
                     VNFILTAVPYRTVWRYGQRGYRYLYLDAGHIGQNIHLAAEAIDAGACMIGAFLDEAMN
                     EALGLDGIEEFVIYIASVGKKP"
     gene            complement(69379..70434)
                     /locus_tag="PLW08_00340"
     CDS             complement(69379..70434)
                     /locus_tag="PLW08_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPL51087.1"
                     /translation="MIALIMAGGVGTRFWPLSRKNLPKQFLRLFGDLSLLQMTVERLL
                     PRFPLADIYIVTASSQVELIKQQLPNLPQQNIIIEPFGMNTAPCIALSIEYLKNIYGQ
                     KETMLVLPADHLIRDQEKFLESLNPAEREAEKGCLITFGIVPVYPATGYGYIEAGEEI
                     ASGVRKVLHFKEKPDKQTAEEFLAKGTYFWNSGMFCWSLESISTAFETLLPDVYGLTN
                     EIGKIWKNEGLDKDITSIYAQMPRIPIDIGIMEKANFRGVIPVSYDWSDVGSWKSLAD
                     LTAGDADNNYFSASGKAISAKNNYVVSDKFTALIGVNDLCLITTKDAILLCNKEMAEE
                     VKKIVDYLEQQNRQDLL"
     gene            complement(70431..71051)
                     /locus_tag="PLW08_00345"
     CDS             complement(70431..71051)
                     /locus_tag="PLW08_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPL51088.1"
                     /translation="MKTAKIITLILLLCCNFLFGISSEALYQKIAKTYGSLSSFQAVI
                     KQDNYFAQLDKSITYHGNIYFTKGRMVIRYNKPYFQRLSVSGGIVELYDSQSNTVFRS
                     KMRPEFGKMNPVEILQLYWKKSTVNIQQGKGNQVNVFLKPFDDPVIVSISAVVNSESG
                     IVQSLTWTNRNNDKVTYNFSSIKTNAKIPASVWQYTYPKDVQVVEQ"
     gene            complement(71038..73281)
                     /locus_tag="PLW08_00350"
     CDS             complement(71038..73281)
                     /locus_tag="PLW08_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPL51089.1"
                     /translation="MANKPQRKVNNRKTNKKGNPLTKRILSGIICLLSCLIILSLTVG
                     YISIKDDLDNLKASGNIISWFKEGNSTTNNPVGIFGIVCGYIFIYLFGYWFSLIGFII
                     IAIVALHYFLTPESLKHRQKGYLLLIIMFFLQALIVSKQDIPGVVPLFVYRILAAVFS
                     GIGAKIILIGAMILTAIMLIEYERLKSWILVARENLQRKKEQRTKTVQPTITANNLNP
                     VPKAAEETIPVIQNHNQPNPPPEPFFEDIIKIPKEYKPKPVATEEDADREYQLPSIDD
                     FLESPIKLSERERKEIENQIITTSQVLKSKLAEFGIEAEVKNVNIGPIITQYELEPAK
                     GIKVSRFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNLARDMIYLKDLLLSEQM
                     RQTTSKLAFGLGKDIAGKPIVADLAKMPHLLIAGATGSGKSVCINTIIMSLIMRTKPD
                     ELRLILIDPKRVELAGYNELPHLIGQVVTDADTALETFIWAVREMERRYEVLQEAKVR
                     DIIGYNEKCREDEDLEPLPYIVIIVDEFADLIMTSGKDIEMPITRLAQMSRAVGMHLI
                     LATQRPSIKVITGIIKANFPARIAFQVSSRVDSRVILDMIGAERLLGNGDMLFLPPGK
                     ALPERIHGAFVSDAEIARVCNFLATQPKPKQDFSLVIEKNEGVGFFDYDDELFPEAAK
                     VVVSTGTASVSMLQRHFKIGYARAGRLIDLLERARIIGPHLGSKSRDVLATREDLIRI
                     GVIDEDR"
     gene            complement(73303..74379)
                     /gene="ychF"
                     /locus_tag="PLW08_00355"
     CDS             complement(73303..74379)
                     /gene="ychF"
                     /locus_tag="PLW08_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPL51090.1"
                     /translation="MKAGIIGLPKTGKTTIFNALTRGEQSTDKYAPPSLEANIGVVQV
                     ADERIDKLSELYKPKKTIYATIEFHDYPGIFYKESEISENALLAEIKNAEAFILVLRG
                     FRDEELEGLFPLGTPIEQFQHFQEEMLLYDLIMAEKRLEKIELGYKRGVKTPAVQIEE
                     KALLKIRDFLQNNNPLINLELNPEEEKAIRGFQFFSAKPMLILINCNEDNINAWDETL
                     KTFTEKGYYAYTIAGKFEEELSKLENEEAQMFMEDMGISESIRDRLTHWTYQLMGYIS
                     FFTVGPDEVRAWTIEKGANAVTAAGKIHSDLARGFIRAECFQYDDLITYGSEKALKEK
                     GLFRLEGKDYIVKDGDILSIRFNV"
     gene            complement(74380..75168)
                     /locus_tag="PLW08_00360"
     CDS             complement(74380..75168)
                     /locus_tag="PLW08_00360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPL51091.1"
                     /translation="MKSSSPNSIKPSSKTVLRAGKTLRLNRYLADCGLGSRRKVEEFI
                     TTGRIAVNGNLVQDLSRQIIPGKDKVTLDGKLIQPAKEKFYLILNKPRGYVVSQNDEL
                     GRKTVYSLLPPKGKQLAYAGRLDKESEGLLFFTNDTALINLLTHPKNKVEKVYRVDIN
                     RPLNNMELNMLRKGVEIEGGKTLPAGIFVKSKNANGMTLKIVITEGRKRQIRQMIEAV
                     GAKVTYLKRLQFGPLKLKDLPVGRWRLLEPGEVKALLSLKKEKT"
     gene            complement(75107..75607)
                     /locus_tag="PLW08_00365"
     CDS             complement(75107..75607)
                     /locus_tag="PLW08_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPL51092.1"
                     /translation="MVKKFIYTILLLVVLSSCHYSVYSNAYPHLRKIRILAFENRTSE
                     YGLGDKLLNYLNREIREDGRLKLVTEDPDCTLEGAILSFSENVYSYDAANQVQDYQVK
                     MVVSVTFTDLINNTVLYQNSGLTVMELYAVAEGGTAKFKTKEEAVEELISKLYKTILQ
                     NSIEGW"
     gene            75703..76371
                     /locus_tag="PLW08_00370"
     CDS             75703..76371
                     /locus_tag="PLW08_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPL51093.1"
                     /translation="MAKKFISKAEAAKKLKVSERVIQEMIATNVFESKLVGKNVKIDE
                     DSLNEWLENLNETEEKMLALKRVICHFEEYMRPENIFLDFTAENKYDAIRILSEKAKE
                     LKLVRDARWLYEVVVAREELVSTAIGNGVALLHPRHLHPSKIKAPSILFGRSSEPVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISRLVMNQDILKGLTEAKDAKEIHQLLTVI
                     PEQT"
     gene            76375..76827
                     /gene="purE"
                     /locus_tag="PLW08_00375"
     CDS             76375..76827
                     /gene="purE"
                     /locus_tag="PLW08_00375"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPL51094.1"
                     /translation="MHPQVRIIIGSKSDLEKITPAREILEEFEVSYDLHISSAHRNPE
                     KTIALAQNAKAEGIEIIIAGAGLSAHLPGFIAAHTTLPVIGIPIASGALNGLDALFSI
                     VQMPSGVPVACMAINGAKNAALYAIQILALKDKDLEKKFSAYKKGLEG"
     gene            complement(76832..77581)
                     /gene="gpmA"
                     /locus_tag="PLW08_00380"
     CDS             complement(76832..77581)
                     /gene="gpmA"
                     /locus_tag="PLW08_00380"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPL51095.1"
                     /translation="MYKIVLLRHGESTWNKENLFTGWTDVDLSEKGIVEAHNAGKRLK
                     EEGFVFEETWTSVLKRAIRTLWIVLDEMDLMYIPVHHDWRLNERHYGALQGLNKAETA
                     AKYGEEQVLLWRRSYDTPPPPLEKSDPRYPGFDPRYANLREDQLPLCESLKDTVKRTM
                     PFWNEIIMPRLAAGRKMIVSAHGNSLRAIVKSLSKISDADIVGLNIPTGIPLIYEFDD
                     NLQPVKNYYLADKAELESAQQAVANQGKAKT"
     gene            complement(77595..78098)
                     /locus_tag="PLW08_00385"
     CDS             complement(77595..78098)
                     /locus_tag="PLW08_00385"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPL51096.1"
                     /translation="MSIITKSGDDGKTSLYSGERVYKDDLRVEAYGTLDELNAWIGEC
                     RNHLANPVDKENLLEIQKNLFRCMGQLATKKGTFAKAITQKDVDNITELVYSLEKNLH
                     LKGFIIPGGNPVSAKLDICRTVARRAERKIIALSRKENIAPELLSYVNRLSDFFFVLA
                     RKEENNT"
     gene            complement(78103..78474)
                     /locus_tag="PLW08_00390"
     CDS             complement(78103..78474)
                     /locus_tag="PLW08_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPL51097.1"
                     /translation="MQSVMTHRAPAAIGPYSQAILMNNILFVSGQLGIDPGTSLIPES
                     FEEQANLVFNNIKSILDAAGMGLSNIVKVSVFLQDLDDFTLLNEIYMRYFSAPYPARE
                     VVQVSRLPQNGKIEISVIAMR"
     gene            78944..80359
                     /locus_tag="PLW08_00395"
     CDS             78944..80359
                     /locus_tag="PLW08_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPL51098.1"
                     /translation="MKDIGNEQRSDELLVNQDKTLMDFNLHQSIVNSDLRDIKRDSGM
                     FFTPEWIVDLMVNLIDDTNYVEKEGIKILEPACGLAQFLLGIKRNKPSLFSQAKLFGV
                     EINQDIINYLSNLNIANAIDLNRADYLLWQTRSYYDLIIGNPPYGIPSLSEHYTIKTN
                     PATKEKYKKLYATWFGKYNVYGAFIEKSINLLKDNGQLIFIVPPTFMILDEFKKLRVF
                     LAQNGKTEIIYLGADIFKPEADVSSVVLNFIKSDKYISRLELKEFQDNKIRTLKSNFH
                     WKGEVVKFDTDYTQTLESLCPYRLGDIYNIRISPRTPEIKRSNIIVKEKAPGENDYLP
                     LLNGKNLKCNMINYNNLTGYWVKKQDVNKIRMYFGSPHIVVGLGFRENGKVAAAFDEK
                     CYPWMGDVYHLVRENSLFNCHFDLETEEVVAYLNSDYIKKYVKDTYREITYHLSITQL
                     KNLPVPDKNTLQLIKKENLCE"
     gene            80352..81131
                     /locus_tag="PLW08_00400"
     CDS             80352..81131
                     /locus_tag="PLW08_00400"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI family restriction endonuclease"
                     /protein_id="HPL51099.1"
                     /translation="MSDRIIEDFKRYRDFLVTIPLDKYREELKDIKWVEQDLPKEILP
                     LASIFKYYWEERQFLDFEEWFDTFWKEINTNSPSKEVLNQFKKYYFDKDNNGWFKKGF
                     RARMYRTWVSVLTQLDFCYLFQFLCAREGKILSLECNAALDARGVDAKVGNIGFQVAK
                     ISNRKEARSASKKKTLVTIPYAVFNIEEYEKKIISPRGKDKSGYQNSLKAFQKYFIRL
                     KNGFVVFGEAYLLPIIDNIEDITKVKETVDKISLELSGEEV"
     gene            81467..82747
                     /locus_tag="PLW08_00405"
     CDS             81467..82747
                     /locus_tag="PLW08_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPL51100.1"
                     /translation="MVQEPQSFHEFMPDKVWKWKILEEKVEKVLSLHDFQEIRLSVLQ
                     DSKVLEDGITALIQGQEADTAMKGVLSLSLPDDQLSLLSLRPEGTISVLHHTARITNP
                     GEIHRFYYSGPMFRRINNQQQMEFYQLGVELLGSESILSENEVISLGMKLCKTLGLNE
                     VRLDLNSYGCFTCRESFFADLNKYLEEHKSEYCNSCYRELSTNPFSQVHCTNPDCKKS
                     LQEGPHIYDYLCNSCKTDFNKVKNIQANLGNQYKVNPNLNKNFAYYNKTVFDFIAKHK
                     GKEIIIGGGGRYDYLSALITGKRIPAVGFYLNLDSIFEIMESRGLFEEKDPEFTVYIC
                     AESPNLEMMTLQIVQELHSQQIKTIISPECRPAENEIAIAKKKDCPIMLILRDDNIRE
                     GKILLRNLVKEEQKYISLKDTLPSIMLAKKAMQS"
     gene            complement(83142..85718)
                     /gene="clpB"
                     /locus_tag="PLW08_00410"
     CDS             complement(83142..85718)
                     /gene="clpB"
                     /locus_tag="PLW08_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPL51101.1"
                     /translation="MNTQRFTIKSQELLADMQDLASSYGHQEIKDLHLLKAMLTQEEG
                     LIQPALEKLEVSIPKVKSEVENALNKLPKVSGSSQQYLSQEVLDILHQAEKEADQFQD
                     DYISTEHLLLAILAKGKEAANILKNAGLQADKLLLALKELRGNQRVTDQNPEAKYQAL
                     EKYARNLTRLARMEKLDPVIGRDEEIRRCIQILSRRRKNNPVLIGEPGVGKTAIVEGL
                     ARRIVARDVPENLLDKELLELDMAAIVAGAKFRGEFEERLKAVLSEVEKSEGRIILFI
                     DELHTVVGAGAAEGSVDASNMLKPALARGNLHCIGATTLTEYRKHIEKDPALERRFQP
                     VLVTEPSVEDTISILRGIREKYEVHHGVQISDNALVAAAQLSERYIADRFLPDKAIDL
                     IDEACALLRMEIDSLPVELDESERRLRQLEIERLSLAKEKDPLSQERLEKIKEEIAQL
                     NEENKALRLRWEKEKQVLEQIRTLKTEIDTLKGEADKAERAGNYEKTAQLRYGTIAAK
                     EKELQKLLQDEAMTKKDRLLKEIVDEEVVAEVVSKWTGIPVSKLAESEMQKLIELENV
                     LAQRVVGQEEGIKALANAIRRSRSGLSDENRPIGSFIFLGPTGVGKTELAKTLASYLF
                     DTEKALIRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVLLLDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVDFKHCVIIMTSNIGSQEIYEASDSDLEQIKPR
                     LMNILQQYFRPEFLNRVDDIILFHRLNKDHIKKIVKLQLDILASRLANRNLQLDFSEN
                     LIEHLAEAGYDPAFGARPLKRLIQKEIEDTLAKYILAGQFTSGKKIMLDWEPHQGLVI
                     TQ"
BASE COUNT        27337 a        16009 c        15569 g        26676 t
ORIGIN      
        1 atttttcatt tcggaaagta aatctcctcc accaacagcc ataaaaaaca ggtttggatt
       61 ttcttgcact gccagagagg cagctttcag aaaattgggg tagtccttgt gacctgcaaa
      121 agcagctaca gttccaacaa taatactttt ttcaggtata ttccaggttt ggcgaaaatc
      181 tgtcggaacc tgaatagtat caaatttatt caggtctatt ccactataaa taacaacaac
      241 cttttcagaa ggaacaccac aggaaattag aactttgcga atattatccg aaacagcaac
      301 aattttatta actgctttgg ttttatactt aaaagccgat aaaggatttt tacctatagg
      361 aaagtcaact cttcttgtag ctacaagttt taagctcgga taaaagaact ttgcccaaag
      421 accccaactg agagaatgac tgctatgcag ttgtaaaata ttataagaat aacgacgaca
      481 aagacgagct aaattaaaag ctgaaattat atctgattca cctctgaaca taagacaatg
      541 atgaggtagc cttttttttt caaaatactg ccgtaattga gaaccaggac aacaaacaaa
      601 accacattta taatcacttt taagcatccc ttcataaaga tatactgcct gttgttgacc
      661 ccctctccaa tgtttttccg catcaatatg aagaactttc attttttcat tttccctaaa
      721 agatgataac gataccaaac gccccaagcg gaatttttac acagtaaaaa acccttggca
      781 ccatccagaa aacccagctg gaaaatgaac attttaagga acttgaagat gcctctaacc
      841 caggcaataa attcattagc tcttttgcct tgcgcataaa gctgtttggt acttaattct
      901 ccatacaaac gcattttagt ataatgaact tctgctgtag gattagtaaa atgataaaga
      961 taatgcttac aggacttaac tttttgcgag gtctgcactc cttcatgaac cagtttttcg
     1021 ttaaattttc cctgctttct gttaaataca cgcaatggag catcattttg ccaaccgcaa
     1081 aaatgaattg gtttacccaa ataataagac cttcttttta gccgccaggc agtgttctca
     1141 aatcttttag ttttcagctc ctgcagttct ttttgcagtt ccgagctaat ttcttcatca
     1201 gcatcaatag acaaaatcca gtcaaaagta gcaaggtcta ctgcttgctg tttggctttg
     1261 ccaaaacctt cccatttatc caaataatat attttagctc caaattgttt acagatattc
     1321 actgttgcat cgctggaacc ggtatccaaa atcactattt catccaccca agaaatagat
     1381 tttaagcagc gagcaatatt ttgttcctcg ttttttacaa tcaaaactgc agaaagttgc
     1441 tccatttacc ggtttctgcc ttcttcaact actttggtgt attcaacttt aaaacggtca
     1501 taattaaaat gctcttgcac atattcccga gctgaattac tcatctgctg taataatttc
     1561 ttgcttgaat tcaatacctt aatatcagag ataaattcat ttattatttc ctgttcttct
     1621 ttggatttaa ttaggaaacc atttattcca tcttgaaagg attctctcac tcctcctaca
     1681 tcagtagcca gaggaaccag accacatgtc atcgcttcag ccattactaa accaaaacct
     1741 tcggaaatag aacatagaat taaaatatga aaatccttta ttgtctcata caacttctga
     1801 cggtcacaaa tttcccctaa atttattacg ggctcggtat taatatggtc gccaatgcag
     1861 taaaatttta ttttcggaaa ttcccgtata attttacgag caacctgctg atataagtaa
     1921 aatcttttct cttttgcctg gcgcgcaata aaaacaattt ttaggggtgc ttgataatct
     1981 ttaggggaaa tatcatcgca gagaggaaca gaattgtaga ttaattttaa gcgactatta
     2041 tatttttcct cccatccatt agctttataa tactgttgca cttcatcata tactgcctga
     2101 gagacaacaa tacgtttgtt aattttggga gtagctaaaa gccagatttt aggatttatg
     2161 ccgtagaaac catgcaaaat attaattaaa taactcttag taagatagtg agataattcg
     2221 taaaatatag tgctgttagc agaaatcact actgatttag gatgcctgtt tatcattgaa
     2281 gccagaaatc catagaagaa attcttaacg ataaaacgat tggtgttatt aaggtatttg
     2341 tatatttcaa tcactcttcc gcttttttgc attgcctgat aatgggtttt gttacgcgaa
     2401 ttcttagtaa ttataataaa aggcttttgg tctgcaaaaa tacgcaaaat gtcaagatga
     2461 acctgttctg ctcctccagt atgaaagtga ggaaagaaaa aaaagaggga actgggatta
     2521 ttccaatgta aaatcttatc ggccaaaacc cccgtataat agaaaggata acctaatact
     2581 cgctctaagc tattcattct aatatctcta aatcattacg aatagaactt aatccttttt
     2641 ggcacctata atttctatgt gaaatctttt ttttacagcg ttcgctataa ttggcatttt
     2701 ttctttcacc ctgatcgtga taatgtgtat gatacaggtg aagagggatt tggttagaca
     2761 caaaattaaa accgcttctt cctaattcct gtaagcgtct acccaaatca tcatcctcat
     2821 tcccccaacc aatatagttt tcatcatatc cattaactaa ttcaaaatca cttttaaaga
     2881 tagcagatac acctcctcgc aattttgccc cgtgtccacc aagatgtaga taacgaaaaa
     2941 gcaaatagga tagaaagtcc tttttatact gtttttgtat ttttctcttt tgttctgcag
     3001 ttaaaattgc attccagtta ttctgttcta taatttcaaa agttaattta tcgctttgtt
     3061 ctttactcaa acgcacagga tagccgctta aaaactttct ctggttaaga tttgccatta
     3121 tacattttaa ataatcctgc ggaatcacaa tatcctgatc caggaaaacc aaaacatcgc
     3181 ctttactaac ggaaacaccg ttgtttctta ctctggcagc gcgaaattga agatgcttct
     3241 gtctcactaa aataagcgga attgcggttt tccttttttg agcgttataa aaagcaacaa
     3301 tatcttcatc cgaaccatca tcggaaagga taatttcatc cggttgcaca ctttgtctat
     3361 acacagaaaa caggcatttt tccagtaagt tcaaataatt aaatacagga acaattaagc
     3421 ttatactcat cgtttcaata aaatatagtt tttgaaagtg ccaatctgtt taccgccatt
     3481 aaaatgtttt tctataaatg ttaaaaagcg gtatttaaag gtttcgtgct tatgtggttc
     3541 acgataagga ttgggtttgc ctgtaagttg caatttatcc tgccagtcaa aacaggaaat
     3601 cctttcctgc ataactttag gatgagttcc gttaaatact gccaatagcg acaaaggtcc
     3661 ataatcaaaa tattgaggtg cattattata ataagcatct gctttttctt tcccccagtg
     3721 cacagaatcc aaagcccggc gtttgttttg cattaattga ggagggcgta cccaaccgta
     3781 gtgataaatt tctgcatcaa ctttagcaac tttcagctta cgggtacctt tttcctgtct
     3841 gggattatcg taatattcaa aatagcgaaa ggattgagca ctctgataag aatgaatatg
     3901 tgggtcgtta cggatgattc ttatttcaaa aggataccag ccatgcccat tttgatagtg
     3961 gtaataatca ccccaaaaat gtttataggc aaagagtaaa ccttccactt ctttattgtt
     4021 taacagttct tcacagcgtt tttttatcac cggtaaatac ttttcgtgaa cgacttcatc
     4081 cgcttgtaga tagaaaagcc agtcaccttg acattgattt ttggcaatgt cagtttgcca
     4141 ggcattaatt actccccttt tacacaattt tgggtcccat tctgtatcaa taattttgat
     4201 tttcggttcc tttaatgcca aaatgcgttc tttagtatca tcatccgggt cactcttacc
     4261 tattgcgatt acgaattcat cgcaaatagg caaaatgctc aatatggact ctatcaccgg
     4321 gtagtatagt tttattccgt tgcgtacaaa tgaaaatcca cttatcttca taattgattt
     4381 tcccctttct gccttttttg taacttactt accctgttgt aaattataga gatgctgata
     4441 gcgaggacaa ttttgcatta attcctgatg tgtgcccata cctacaattt ttcccttttc
     4501 caaaactact attttatccg ccttaagaat tgtagaaagg cgatgagcaa ttaggataac
     4561 ggttctgttt tgagttgcct tatcaattgc catttgcact ttctgttcgg aatctgtatc
     4621 aagtgcagaa gttgcctcat caaaaattaa aataggaggg tctccaacaa tggcacgcgc
     4681 gatgcaaagt cgttgttttt gtcctccgga aaggtctgaa cctttgctgc cgagaacttc
     4741 gtcatattta ttcggcatct catttataaa ttcttccgca ttagcaattt gggcggcttt
     4801 aatgatttgt tcgtcgctaa cctcttcctg gcttccgtaa gcaatatttt ccttgataga
     4861 tttggaaaag agaatggaat cctgagttac aacaccgaaa aacttgcgta agttatccaa
     4921 tttgatatct cttatatcta ttccatctat tttaatggct ccgtaagtaa catcatagag
     4981 gcggttcatt aagttggcaa tagttgtttt tccaccccca ctggcaccga caaaggcaac
     5041 tttacttcct ttaggaatag ttaagctaaa atttttgagc accggtttgt tttctttata
     5101 gccaaaagtt acattttcaa aagttatttc gctatcaaag gtctctttgg taatggcatc
     5161 aggtttatct tgaatgcggg attcctgatt tagtaccaag ccaattctat ccagcgaaac
     5221 aagagctttc ctaatatcag tatagacctg agtaatcact tttagcggat gcagcataga
     5281 aaaaagagca aaaaggaaag ctgtaaaatc acccaaagaa aaactgctgc ccggggtaag
     5341 aatcattttc ccgccaataa tgattactat tatgccggta attgcagtat ttaattccga
     5401 aatgggagtg ctcaaagttc cgtaatattg tgccttaaac cacatatgat aatgtttatt
     5461 gttgattgct tcaaaattct ttttctcata ttgttcgtgt ctgaatgcct gcacaatttt
     5521 catactattc agcacttctt ccacattaga gaagagattg gaaagctgtt cctgaatgcg
     5581 tttggaatat ttttttatct ttttgcctaa aaaacctatc gttaaagaaa ataccggtaa
     5641 aataataatg ctgtatagaa gcaaaacagg attcaataaa tacgctattc gggcaaaaac
     5701 aagcaccgta atcatttccc gaattacatt cacaaaggaa ttgattagct gttcactgac
     5761 aatttctaca tcacctacca ttcggacaat agcatcccca atgcggtgac gactgaaaaa
     5821 agttaaggac tgatttaagt aacgggaaaa catataattg cgtatatcgc gaatggttct
     5881 tcctcttacg gaattaaaaa atatcttgtg taaaagataa aagacattct tgaaaaggat
     5941 aactaaaaaa accgctatac acaataaata caataaagaa agggaatccg tttgcagcat
     6001 cagcacttta gcatcttccc ataaggcact ataattttca attgccggga aaaaaccgcc
     6061 ggaagaacta atatgctgtt ttagcatctc tcctaaagcg taaagaaaac tgctaaaatc
     6121 ggtgtataac aaattgattt tatttggctt gaacacataa tcaaacaggg gaataactaa
     6181 agttacgctc actccattaa aaagagcaaa aagcaacata cttataaaac cagcaagcat
     6241 ataatgccaa taccgcaaca tcatcctgaa aatcaggata atattattcc atctgttctt
     6301 gatttttcgt ttcattgcca taattaatcc acaaaaaaaa gccagcaact gctggcaaga
     6361 aaaaagtggc acgccctaag ggagtcgaac ccccaacctt ctgatccgta gtcagatgct
     6421 ctatccaatt gagctaaggg cgcacattaa tgtcataaaa ttttgtcttg ttaatctgtc
     6481 aacttattct tgcaaaagaa cctcactccg ttttatagta tttaagcagt aattgatacc
     6541 tcatattttg cctaaaatag aacaaaacac gaagatagga ttagcatttc ttttttctct
     6601 ctctcagtat tgattattac atctatttca gcaaaacgac caagttcttt tcttttctcc
     6661 tgaagaaatt ataagcatct tcggcaattt accaaatatt tctcttccaa tgcttatttc
     6721 ttgctttaac ctgttaagac caggtaacat ttatgtaaca acgccgtaac gaaactactg
     6781 cagtgttagg gaagtgttac ttggtcgtta gagaattgac tatgtagcaa gcaattaggc
     6841 aatcccaata atgccccgtg aaatcgggaa agtttttatc tcctaaaaac actgtaattg
     6901 tctaaatcac agagagttat ttatagcttt ccggtgcttt ttatttatca ttcttcattt
     6961 ttcgttctat ccgataaaca gttttacgag gatagctatc aggagtatgg aattgattgc
     7021 cagccaaatt tttattttgt taaagctttt gatatatatt tcccggtttt gctgaacctt
     7081 tttttccaga cgaaccatta aatgttccag ggaaccaatt ttttcagctg tccgatcttt
     7141 ttttctgata ttttgatcgt gtttggcatt tcgcttataa atatggatca attcataagc
     7201 aaaagggata aaagtaggaa ttaatttctt gaatttcata ctaaccctca tttgaattta
     7261 tagcccaaag aaaaataatg tgttccgggc aaaacactat gataggtaaa ggaataatcc
     7321 acttccaaat tttggatata gatgcttaag ccggcattat aggtagcggg attttgatgg
     7381 acaccgaagc gaattgccaa gggctctaat aaatgtgctt ccactccacc cataaattcc
     7441 gtttctttag caaagtcctt tttcacttct atagaggtag taactttctc ataaggaaca
     7501 taagcaattc ctaaagccaa tttactgggc agatctattt gattttcatc acccattgtg
     7561 gctttattaa tattggtaac agcaaaggca atatgggttc tggaatgcaa aacagctaaa
     7621 gctcccaggt ctattcccaa ggcagtatcg tcatcttcac catccatact taagaggtac
     7681 aaatttcctg tataaccgaa agaaattcgg gaatggatat cctccagtaa aatgaaacta
     7741 tgtgccaaac tatacacctg ctcactcatc aggttaacat cttcaaaatc cacatccatt
     7801 gctcttgcac caatagcaac tgttcccaga tttttaggaa gtttataacc caaagcaact
     7861 gtcttcaact cactgaagtc ctgattgtaa agctgggaaa agctaagttt agcgctaaaa
     7921 gtagtattaa tcagcccagc aggattgaaa aatatggcat tggcatcttt agaaacagct
     7981 gtataggcac cacccattcc tcttgccata gcagatggtt gatagtcatc aaacacagct
     8041 aaaagtaata cggatgggat tataagcaga cagacaaaca ataatttttt cattataaac
     8101 ctcacttcaa agcactttta attacaatgg gctgaactgt attgtctttt tccccgctac
     8161 tccgatcggt aacttccaaa tgacaataat acaaacccgg aggtactaat ttcaaattgg
     8221 catcgcgtcc attccattta aattcctgaa tacctgtggc agaagttata tttgtatgca
     8281 caggtgtata aaccaaacga ccttgagcat cataaattct tataactgct ttggaaagat
     8341 aaccggtttt ggttccgtag ctgataataa tttcttcccc catttccgga ttgaagatat
     8401 ttttcttgtc cccttgttta ccaatattca gataggcaat tttcttcggt actttaacaa
     8461 ttacagagcc atcaatcagg ttattaactt ctgtcccatt atatttaaag gagacaaggt
     8521 caattatgtt ttctccataa acaagaggtc taaccagaat tttaattaaa agagcatcat
     8581 tatcaggata aataatcccg tcctgagaaa aataagtcca gctgatagta tctccagcag
     8641 cactaatatg aaactcaggc atttcacgag ttaaagtatt ctcaatatcc aaccccgaaa
     8701 aaataagctt ggtgggatta actttgaaag taacggaata actcatcact ccccattcgg
     8761 acttaatttt ggtgctaatt aaattcatag aaacatcaga attaagggtg ggctcagttg
     8821 taggatcggt tcttactatt tttataatgg aattctctat agtatcctgc ttaatcatat
     8881 cgctggcatt acgcggattt agtttaaaac ctgcactgct gtgaaaatct acaatcccct
     8941 gaataatata ccaactctca cctacaacaa ttgaagttgc cggaatggca tataatccat
     9001 tatctaccat agcccaaata ttactgtgat ccgctattaa aaattgacca taggtatcgg
     9061 gagtggactt aattgttaca tcctgaaatc tgaccagcac tccttcatac ttctcatttt
     9121 caggatgatt agttccggaa ggcatatctg ctgtagataa ttctaagaaa ggaggtaaag
     9181 ggtttccttg agatattatt tcataagcac ttacattggt aaattcggtt aagttataat
     9241 attcatcaac tgttccggta agctttacca gatcacccac actaacaaca ttgctggtgt
     9301 ttctatgata aacataaagt ccgctccatt cacccccttc tgggtctgca atataaaaag
     9361 cagaattggg tataatatga gttacgattc cttgaacagt aacagtttgg gtatagtaag
     9421 gcgaattacc tgtaggatcg gtagtatatt gaatgtcgtg acaggaaaga actgtttgag
     9481 cagatattcc tgccaccagg aaaattgcca gaaaggtaag aatataattc ttcatttata
     9541 gctccttatt aaattcactg atttctatat gttctcctgt ttcgggaaga gggctcggct
     9601 tatctttgat aaacagcata tcaatgatca tcaaaaccac cccgataaag attgaactat
     9661 cagctatgtt aaacacggga aaacgttcca taataaaatc gggaaaatcc atagaaaaaa
     9721 agtccgttac cggtccaaat aaaacccggt ctatcaaatt tcccaaagct ccacccaaaa
     9781 tcaaaccgaa tgcgataact tgaatcttgt gttgagcata atgtaataaa taaataataa
     9841 tacctacaac cagaaccgtt acacagataa aaataatacg gttgactaag tcactaccaa
     9901 aaccaaaaga aaaagcagca ccgctatttt gcactctaat taagataaag gtatcaccaa
     9961 ataaattttg caacaaagga atgtgttcat acatttccat atttactcgc accaaggttt
    10021 tggttaattg atccaaaact aaaatcagca gcataataaa ataagccgga gctgttttta
    10081 actgtgtacg aggttgtttc aacgcttttg ccgccttttt ttgtcttcgt ttttctcttt
    10141 acaggcaaca cagcaggtgg cataaggaat aatttccaaa cggttttcct gaataaattc
    10201 accgcaaaat tcacaaatac cgtaggttcc atcacttatt ctttgcagtg cttcattcag
    10261 ctgacgaatt ttttctctct cgctttccag catcataacc gactgctcca taaggttagt
    10321 atccgaacct tgatctgctt gatgaaaagc atagctggaa agatcaccgc tactttcacg
    10381 agcacccaaa ctttgttctt tattgatgct ctcaatataa gcaatacttt cctctaattc
    10441 agcccgaata acttcttcgt agtgtttcag tttttcaggc ggtaacttct tcgttgccat
    10501 tatattctcc tttggttttg ctcttttaaa aatcaaatta ttcaaacatt ataataccaa
    10561 ttatgatgtc aatgaaaaag tccttgttaa ccttaaaatc aattctgcta ttagcttttg
    10621 tagctgcttt cccgatatat cggcaattct ttgaatttct tcttgactat gtgccaaatt
    10681 atgaaacaga ttactatagt tagtcacaat tgaataggca agaactttaa gccctgaatg
    10741 gcgagctgtt ataacttccg gaactgtaga cattccaaca aggtctgcac cccaagaagc
    10801 aaaagcagag cattcggctt ttgtttccaa agaaggtcct gtaactgcta tataaacacc
    10861 tttagctgtt ttaattccca aatcaaaagc tactttatct gccagcaaac aaagttccgg
    10921 atcatatacc ttattcatag agggaaatct ttcacccaaa tcatcatcat tcattcctat
    10981 taagggatta gtgcccataa agttaatatg atcctgcagt tggacaatag ttcctggttc
    11041 cagttctttt cttaaacttc cggaagcgtt agtaacaatt aaaaccttta ttcctaatgc
    11101 cgataaaacc cttaccggga aaacaacttc ctgcattgaa taaccctcat aataatgaaa
    11161 gcgtccctgt aaaaaaataa caggaattcc ttcaacttca cataacagca aatttccttt
    11221 atgcgtagga gcagtgcttt gaacaaaacc gggtatttga ttataaggta tagaataaag
    11281 tagcggaaat acttctgcca tattacttaa tcccgttcct aaaataactg caatttgtgg
    11341 ttgtttaggt aatttggttt tcagccaggc agaggtatct ttatagctgc tcatttattt
    11401 gttcttctcg ttttgtgttt ccgtattttc cggtttgggc acaatttctt cttttatttg
    11461 atgaaattca ctatctaacc tcaattcact gtctttgctt aaaagagggt ctttatttat
    11521 tctatcctga aaactttgga gttccacctt aaactgcatc aggaattgtc ttttctgttc
    11581 ttttagttgg agatattgat gttccagcac gcttaaatat tgttttgctt cctggatagc
    11641 tcttttagct ttcagctctg cttccttaat aatattttct gcttccttgc gagcgttggc
    11701 tataatatcc gctttggttt tttctgcaaa aacaagagtt cggctgatta attcctctct
    11761 tcttttcagt tcttctactg cactaccggc ttgtaatgct tcttgtttca cttcattttg
    11821 tttttgttcc tgacgggcaa tttctttttc cagctgtttt tgaaatgctt caacctctga
    11881 agctatctgg tctaaaaagg cacgcacttc ttttttgctg tagccaaaca ttgcagtgga
    11941 aaactcctga tgtcttatat ccaaaggaaa taggggcata aattcctcca ccttaaatta
    12001 acgaagttaa aagacgagat aatagatcaa gaataaaaaa tgcaattatc ggagataaat
    12061 ccaatcccat catcggaggt aaaatatgac ggattggtcc taaaaccggt tctgtgatac
    12121 ttattaaaaa gtgatataaa cgattatacg gattccgaat tatccaggaa gcaaaaacgc
    12181 gcgctataat taaaatatta tagatctgaa taatccgaac tgcaaaaagt aatatggaac
    12241 ccgttaagct ggttgaatat gtcaatccaa aacctcatga caatttctgc atcttgcttc
    12301 ataagcatct gtggaaccaa ccagaatctg ctcatcctta tgaattgtgc gttgagttct
    12361 attagcagga ttaccgcaga ccacacaaat agccaaattc ttggtaatat attctgcaat
    12421 tgccattaat tgaggcatac ttccaaaggg ctcaccttta taatcctgat ccaaaccggc
    12481 tacaataacc ctataacctc tatctgccaa atcattacag ataccaacaa gggaaggatc
    12541 aaaaaattgt gcttcatcaa tagctattat ttttacatcg tcatccagat aactataaat
    12601 ttctccggga gcatttatcg gaattgaagg cgcctgcatt tgtgagtgag aaacaatatt
    12661 attggcatcg taacgattat caataaccgg cttaaaaacc agcacttttt gtttagcata
    12721 ttcagcccgt ctgattctcc gaattagttc ctccgtttta ccactgaaca tacttccgca
    12781 aataacttct atccatcccg ttttctgatt tacaatattc aacattcctg ttcctccctt
    12841 agaaaatata ttttaattac caagtcctgt atctcccctt ttttgtcaat tcttcattta
    12901 cctcatccct ttcgtaactt cccttatggc agaggaaaac tattatagaa caaggatatt
    12961 ttatttatag accccgctaa aaaatgtaat tatattttcc caaacttacc ggaatactta
    13021 cctgatcagt aattataaag cgctttactt aataaaggaa tgatattatc caggtaacaa
    13081 aaaacggact tcctcctccc acatagtttt cctatatctg aaaactataa gaaaatacgc
    13141 gtaaacacca cttcccacaa aaaaaacttt ttgccggcgt agcttacaaa tcaaagtccc
    13201 gttgtcttat ttgtttatca ctaagcttta attactttta aatatccttc aggcaaacat
    13261 aaagttaccg ttcccacttc atttcctaca gttcaaagtt ctgtttcctt tcctgaaaat
    13321 atcatcctaa ctgaaaacag aaacaaaata aaagagaact attctctaaa aacaacacta
    13381 cagtatcaat ttcctgttac tatggtttgg tatttcatca gaaagagaag ccaggtagtg
    13441 ttataatcaa cagtagaaat attagttatc ccaccatttt cagctgcctt ggcaatagcg
    13501 gcatctgagt tcgcaacagg ttgaaagaac aaaataccga tttgagtata aacaccttgt
    13561 ttttcgccca acgggttaga agtagcgcaa atcggtttgt tgattgtgca ggaactgata
    13621 attaccagta acagcaccac tgcgagaagt tttggtagtt gtttcatttt tgtttctcct
    13681 ttatattaag acacctgtac gggtcttata tttataatgt gcttgatagg aatttaaact
    13741 tccgcttttt ttgaaacaaa aagctaggtt tcatagtaat agatgacagt tctcttcaat
    13801 agtattttaa ttttggagca taaccctgaa taatgtaatt cgggtaaaca aagtatcctt
    13861 tcagccacgc aattttccat actcacactc cttttattat ataattttct tgtaaatata
    13921 attataaatt tgtcaagtgc aaattttttt cttctacttt ggaataaaaa aagattagtt
    13981 caagctaaga gtataagcta aaatagcaga agagatagat acattcagag attccatttc
    14041 tccgggcatt ggaatgcaga gtgtttcttc ggcaattttc agaatagagg aagatatgcc
    14101 tttagcttcg ttgcctaaag caactatata aggttctgaa ggtggtgcaa aatgagcgag
    14161 aggaattttc ccctgcatat ccaaagcaaa tatatgtgcc tttaattcct ggagattttc
    14221 cacctcacaa aagaaaaaag gcaccttata caccgctccc aaagaggcac gaatcacttt
    14281 aggagagcta atttcacagg tcttaggtga aagaattaaa cagtcaatat taaaggcagc
    14341 ggcaattctg aaaatcgttc ccaaatttcc cggatcgctt atatcgtcca gataaaatgc
    14401 cttcttaaaa gctattttcc ggggtttagg caaagaaaac aaacctgcta ttttttgcgg
    14461 ctgttccgaa tcgcaaagtc gtttcattcc ttgttcggaa acggtataac ttattgctgc
    14521 cgaaacggtt ggttcaccat caacgagata tagttccagg ggtttaatgc cccaagcaaa
    14581 gagctgttcc aaagttcttt tcccttcaac aattaccaga ccttcctgca aacggaattt
    14641 cttctgtttc agtttagaaa gatagcgcag atgttcatta ctcattttag gaacggcaac
    14701 attttctctc atcaactatc cttaattcca agttctctta attttaatca gatagggact
    14761 tatccacaag ctctgtggat aactataatt tattaactgc ctgaaaataa tagctgttat
    14821 attttgtaga tgactttcca caaaacttat ccacaggtta tccacattat tcccaaacgc
    14881 ataattttag tataagatag tcagcttttt tgtaaatttc ctgtttccca ggtgcaatct
    14941 taaaatgtaa actccggaag gcagagctct aaatttcggt tctgcagaaa gcaggaaatt
    15001 gccttcaccc gtaattactt ctcccaaata tctatttatt actttttggc ctttcaggtt
    15061 ataaatttcc agctgaattt tacctgcaag ttcttcactt gcccaccacc gtaaaacggc
    15121 gagcgatgtt ccggaaacag gattcgggtt aatgatgatt gatgcctgtg ggttagaata
    15181 ggtataattt ccaattaagg gttggctttt gttgccactt tggtcaacag ctcggatttg
    15241 ataagttccg ttttgggaga tttgtagcat aattgtaaac acagcattat cgggattggt
    15301 ataaaaatat tgaaaatgct gtccctcgtc tatggttatt tcatagtaaa gggaatcggg
    15361 atcgggattt gcctgcagag aaatttgcaa agtgttataa atcagatttt gggagatgag
    15421 aataggtgtt tcaggaggaa aagtatcatt tccgatagcc gaatttacaa aactgagcca
    15481 acgggagcga gaaaaattca gcttatgata ttgtctccag ccgttttcaa aattgaatct
    15541 gctatcggga aaccaaattg cagctgtacc aatattacca acttcttcag gattggtaaa
    15601 acaactgctg ataacaatag aagtccaaag agagagcaaa ttttgtgttc cgcttgtcca
    15661 aatcgtttgt gccttttcca aaaaatctct gatatcaata tctgcaagtc ctgtgttcat
    15721 agaacagcag gaattgcgaa cagtaaaata tttaggggca agaaatcttt ggctgatagc
    15781 aaaattttta atctgttgca caaactgagg cactaattct gttttcacca ctgaacaggt
    15841 aacagaccaa agagctcctc caggatttat accctgtcct ggcaaatagg aagaaacata
    15901 gagttcggga atttgcgcta acagctcatc aaagtcacca tcaaaaaggg gaataatatc
    15961 ttcataagga aaaccatatt ggggaacaag gtcttccgaa ccgattatat aatccgcaaa
    16021 gctgtgaatt tcattgatta cttcaatgca ttgcatactg caagcatcaa atagtaaaat
    16081 atctaaatgg ggaattcctg aaagtgccaa aaccagttct cccccggaaa cactaatcag
    16141 gtcttgagca ttatcatcgg gacaaatcca ttttccgctg ttgtctttat accagctatc
    16201 cccatgtgac caaataataa gcattttgcg ctgagcggga tagcggttga agccccattt
    16261 tataaaacta tttaaggtat taggatctcc gctattgatt gttcccaggt ttgctaaaac
    16321 cggtgaagtt atttgttccg aattatcctg ttgaatttta tagcgttttg ctccttccgg
    16381 aaagtcagct tgcacaatta agttcaaatc cgcaggttgg gcaacacttt ccagagaatt
    16441 tatatctaat ctgcccattt ctgccaaatt attatcggca gccatataaa gaaggacagt
    16501 ccaactttct gcactaagga aaatgccaat agtaagcaga aagataatca ggaaatattt
    16561 caataaggat attcctttgc ctcttcggtt ccattcagaa ctctaattcc accctggcag
    16621 agagcttcca tttctttttc gccaggatat atatatatcg gagctataaa cttagtatgc
    16681 tctttaataa aatttactat ataatcgtta tatgccaaac caccgctcag ataaattcca
    16741 tccacttttc ccgcaagcac tgctgaacag gaaccgattt ccttggaaat tctatagcac
    16801 atagcatcta aaacaagttt agctttttgg tcaccttctg caactctttt gccaacttca
    16861 attccgctgt cggttcctaa ataagccata agacctgcac ccttggaaag ataagtggta
    16921 agctcttttt tagtgtattt acccgaatag cacatttcta tcaaatcgcc gatgggaaga
    16981 gagccagctc tttgaggaga aaagggtccc gtacctaaaa gagcattatt aacatctact
    17041 actcttccgt tttttatagc ggcaacggaa attcctcctc ccatgtgcac tccaatgaaa
    17101 ttaacttcag ttaagttctt tcccagtttt gctgccagta aacgacaaat atagcggata
    17161 tttaaggcat gaaacaaact tttatgttca atttcaggaa tgcctgctaa gcgcgcaata
    17221 tcctccattt catccgtggc tacaggatca actataaaat agggaatgct aagtggttcg
    17281 gcaaccgctt ttgccaaaaa ggcacctaaa ttggaagcat gacttcttcc ccaaattgta
    17341 atatctttca gatcgcgtaa cattgcttca tttactttcc aggttcctcc acttacagga
    17401 cgaactaaac cacctctacc aactacggca tttaaacttt ggggatggac attattttct
    17461 tccatcgctt ccataaccaa tgccttacga aaatccgtct gattaaaaag atctccaaat
    17521 tggttaagtt cttcaggatc gtggcgtaag gtcttttcaa aaacgggggt gctgtcatca
    17581 taaacagcaa ttttggttga tgttgaaccg ggatttatcg cgagcacacg ataattcatt
    17641 tttcctccag aatgttaatt ttgttttgtt tatcttaaaa aatacagggc ttaaaaccgt
    17701 caatcaattt tctttccctt cccctgcttt ctatatctgc tttacttaaa aacaaagatt
    17761 tggcttgcca aaaaatatgc cttccaaatt atgactaaaa tataaacaag gaaactccaa
    17821 tgaccccaaa agaaaaaaac gaaacccaac ctgcagatac acatatctgt gaagaagaat
    17881 gtgagcatga gcaatttgtt attcccgtcc aaagcgaaga tggctctact caaaattaca
    17941 tccctatagg gcatattgac tatgaagata aacaatatat tgccttagtc tctgaaggag
    18001 aagatactta tgatatctat caaatgatcg gagaaggaga tattgttact ttttacccca
    18061 ttgaagatga ggaattgtat gatattatag ccgataagtt taacgagctt ttcttatccg
    18121 aaatggacaa aatgggattg ctggacgagg aaaatttttt aaatcctgag gaagattaat
    18181 cattaaccaa taaattctct ccctattccc aaaattcccg atagagagtt tcttcgtctc
    18241 tatacgataa gccatttatt taagctaact atatgagagg attgatatta acttgtatcc
    18301 ttctgttctt gtattttcct aattttgttt gctgctaagc tggatctttc ttattaccca
    18361 acaacccatc ctttactatg ggttgaggat gggaggtgaa taaggaactg gaaaccgact
    18421 ggcaaaagtt taatccaaat aatgccattg aggaaaaatg ggttggcaaa ttctttcttt
    18481 gccaaagaat gcatagagct ctgaaaaata aatgcgtttg gtttattgca aattagaatt
    18541 ccgcaactaa cataccaaat ttgacttttc tgtattgaat taaaaacctc tgacatatct
    18601 gtcgtttcaa cggaactttt tttcttgcat gaggatattt ttgggccagg ttataaaccg
    18661 tcattaataa ctatcattcc tccgtaatct ttactccaat atatgattaa ttaatttgga
    18721 tatagtatta aacatcattt ctaaggcatt attggggatt atacatcaca aatatagaat
    18781 acaaaacagg tctgcaaaat taaaacattt tgaatttttc ccttgacagg atattctttt
    18841 ttcagcttaa agaaaacatt atattttatt ggtatgaaaa tgaaaagaaa gatagttttt
    18901 tggtttattt gcctcttctt atgcggtggc tgttggctaa cccgtaaaat tgatacccat
    18961 caatttgcgc ttaaagagat gactcaattt gctactccct taaatggatg gataacagcc
    19021 ggtttgaaag aagaaggtga ggactttttc atttgccatt ctcaatatga tgaaaactta
    19081 tattttgttt tggtaaatga tattcaaggc aaaaccacct cccaaatcaa tctccccagc
    19141 aaaattatag atttgaatac ccttattgat aaaagaaata atgaccaatg gctatttatt
    19201 agcagtaacg attccgattc cctttattta atgggtgtta aatatatctg gcaaattcct
    19261 cttcaaagag agaacaaata tttttcctct ttagagcgga ctgactgggc aaaaggcaga
    19321 gttgattata tatataacgg ttctatttat ccgatttttc tggaggacat agataatgac
    19381 gggaaaatgg atctgctttg ccagttaatt gattttttta ctgctaaacc aagaggtttg
    19441 attttatacg attttgaaac cggctttatc aaatggcgtt atgatttacc ttgcaatccc
    19501 aggagtattt tatgggatga ttttgatgga aatggcagca aagaaatcat tgtctctaat
    19561 gatgctttga aaaatactac cgaaattatc aatgatgtgg atgattgtag tggctggatt
    19621 attgttcttt ctgcaaaagg aaatgtctta tttaaagaaa aagaatttag cggttatggt
    19681 agaatttatc ttgatgctgc agatgttaat caggatggaa ttaaggaaat atatgccgta
    19741 aactgcacat ggggaagcga aaaacacttc aatattgcct ctgtctttaa ctggaatggc
    19801 aaacgactga tcaaaaatag aacattggaa cttccttcaa ctttggaatc aacccaattt
    19861 cccaattttt tacaacaaat ggatagcagg tctgattatt atcttcattt ggtagatact
    19921 aaggcaggat tacttattct ggacacaaat ttgaaccccg taaaacataa ctataaggac
    19981 tttgttaacc aaatttggga tattggagac ctggatagag atggcaacaa agaactgttt
    20041 ctgcaaaaga aagatggctc tctggaaatt ctggataaaa attattaccg tagagcaaga
    20101 attaaaaatc cctttccgga taccataatt tataatgcaa atattgtaaa aacgggattt
    20161 gagacaaagc cattgctcgt agtaggaacc aataaacaaa tcaattatta tgaatataca
    20221 tccctttccg cttttactat tctgctttct ctcctgcgaa attatgctct tcttttgttc
    20281 attctggctg ccttactatg tattattttg attatcagaa atatgtatca taaaaaaaac
    20341 actctaaaac taatagatag ctgggaacaa gggattttaa aagtaaacaa aaaaggcatt
    20401 atcaaacacc ataatttcaa aatcaaagag cttgctaatg attttgaact gctaaaaaga
    20461 aagaaattgg agcattgttt tcctgctttg gctaaacctt ttcaggaatt ttgcaacgcc
    20521 aatttgaaaa caggaagtta tattggaacc ctaaatccaa atccgcaaag caaggtctat
    20581 caagtttact ttattcggat gacttcctac agaaaattct attatctggt tatccgaccc
    20641 tatacggaag aaggtgcaat tgcagaaaaa atggaatggg cagaaactgc ccgtcgtctt
    20701 tctcatcatg tgcgcaggta tataaccaat attttgctcg ctctggaaac tcttaacaca
    20761 gatacagacc ccacccgcaa caaatattat caaattatcc gctccgaaat tgacaaggta
    20821 agggtcttta cgcacgcttt tcaacggttt acggaactgc aagattatca attggaactg
    20881 caggatatca ttccttccat tgaacactgt ttatcaaaaa ttcagtttcc cgaaaatata
    20941 caattgatta agaattggtc gctggcttcc atttctgcct atattgagcc cattcgtttt
    21001 gaagaagcgc tcactaatat cgttaataac gccttggagg caatgcctga agggggaact
    21061 ttacatatca gtgttaagga atttcctctt gcttcttcac cccaaggtca attgagagtg
    21121 ttaattgaaa ttgaagatac cggcaaaggt attccgcaaa aatacctgga agatatctgg
    21181 aaaccgtttt ttactaccaa tcaatccgga acaggtatcg gcattccgga aactaaaaaa
    21241 ataattgatt ctatgggagg gttaataaat attcaaagtg aagaaaatat tggcactgtt
    21301 gtcactattt ggctaaaagg agattctaat gttagaaatt agtatccttt tggcagatga
    21361 cgatcttatt ttccttcagc tgacttccga ttttttaagg aaaaacggct atgctgtgga
    21421 ctgtgcttcc aatattgaag caacaaagac ccttttacaa aaaaataatt acgatattat
    21481 gatgctggat atgtgttttc ctgctcttta tgacggcttt aacctgttgg atgaagtgca
    21541 cgaaaactac cctgatttgc ctgttttaat gatttccggt agcggacata tccccgatgc
    21601 cgtaaaagca attcaaaaag gagcggcgga ttttattgaa aaaccaatag atactaccca
    21661 tttgctcttg cgcctggaac gaatctatga tggaattgta accaaaaaac aaatgcagag
    21721 cttacgctta tccacaattg ggatgcaagg caattctgct attatgcaaa acatctatga
    21781 cgatatctgt aaagctgcca gatttgattg tcccgtttta attacaggtg aaactggtgt
    21841 aggaaaagaa cttgctgcca gagctgttca ccgcttaagt aaatatagcg caaaaaatct
    21901 ggtgagcata aactgcgctt ccattcccaa agaacttttt gaggcagaac ttttcggcta
    21961 tgaaagtgga tcatttacag gtgctgtaaa agcacatagg ggctattttg aatacgctga
    22021 taacaattct ctctttatgg atgaaatcag tcagctccct cttgaagttc aggcaaaact
    22081 tttacgcgtg gtctcggaaa atgaaattca aaaaattggc ggtaaagttc aaaaagtgaa
    22141 tgtccgcatt atcagtgcta ccaatcagga tttggaaaaa cttaccgcgg aaaaactctt
    22201 tcggaaagac cttctctatc gcctgaatac gattgaaata aatattcccc ctctcagaaa
    22261 acggattgaa gatatccctg ttctggcaga gttctttatg aatgaattct gtacccggaa
    22321 tgatattccc cccaaaccaa ttagcccttc tgccgtttcc tggctttgta aacaaccttg
    22381 ggaaggaaat gttcgcgaac tgaaaaatgc agtggaaaga gcggtagttt tttccaaaaa
    22441 tgatcacttg actatggtag attttactac accctctgaa agtgatatta ctgaccgcga
    22501 gtatggctct ttgcgtaaaa caattaccaa ttttgagaag gtatatatag aaaatgtttt
    22561 aaggatttat aattataatg ttcgccaggc agcataccat ttacagatgg ataaatccaa
    22621 ccttttcaaa aagatatcag ctctgaaaat aaccctgcct aaccataaac caatctaatc
    22681 taaaggtcct ttgtcattcc tgcacaagca ggaatccatt ttcactgtgg taaattttac
    22741 cccaccatcc ttttttatta tctgcataac taccataatt atcaatgtat tatatttgta
    22801 aactggtttg gcacaatctc tgcatttaag aaaaacgaat ctttatattc ttgggtttag
    22861 gaaagtagga ttagggtctg taggcacggt ctgtaggccg ttcttttttc cggctgtttt
    22921 cgcgtatttt tgttttccca cagattacgc agattttatt atttgaagaa caattaaagt
    22981 gatactataa atttcattgt atttctaatg ttctatctca ctttttccta tctacttctc
    23041 aacctctcgc cttacaaata cctcagcatt tctctatccg tcactcctgc ataaagctca
    23101 atatacggtt tcaccggact gatattaatt ttgggaaaaa atacctcttc cacctggaaa
    23161 aaacctaccg aagcattcat ttccacagta atattgatgg gcttttcctg ccccttaaaa
    23221 attttcactt tttgaatagc ggaagcagaa gtccggtgaa tatctccaac tctggtttga
    23281 gaagaaagca tcgtaggaat tctggctctg cctttttcca tatcgcttcc atctccaatt
    23341 aaaaccaaac ccgcttccaa agaatgaatt ttatgggttg ccatatgacc aaaaatacct
    23401 tcaatggcaa gagaccgaat agcggttttt aaaacaattt cattttccga atgcaaagtg
    23461 tttaaaactg tatctatata aggaatagca agttgaatag aaagcaattc atgggactcg
    23521 cgggtaattg ccattcccaa atcatgcagc aaagaagcta aaattacagc tgtaagggaa
    23581 tcatccgcag ttcctgctcc ttctttttcc agattcattt taatgcccga ccgattaagc
    23641 aaattgaaca ttttaatagc gttcaaagtt gcttttcgca tatggacagg accgtgatca
    23701 ttaaagccca aacgcttaat agatacaata ttggcatatt cttgacctgc ttgcagttcc
    23761 ggattagcaa aaagccattc agctgtttta agtgcttttc cagaaagcaa cttcagaatt
    23821 ctttgctcca attgttgctg ttttattgat ttatacattt tattaagctc ctaaaatctg
    23881 ttcatcagtt tgttgattaa attggttact gaagacacaa tagcaattgc aatggaaatg
    23941 gttccggcaa taagcagggt ctgtaaaata gctttggaag cgtgcaaatt atccccggct
    24001 atgtataatt gcatagaatt aaagagacct cttcccggaa ctaaaggaat aatagcacag
    24061 gttacaaaaa cggaaaccgg tgtttttaaa cgcggtgcta ctatttccga ataaatgcac
    24121 accaagataa tagctacaaa tacagaaaaa agcattgact tgctataata caaaataatt
    24181 aaataaaaag cccaacacag tcctccactg agcgcagtaa aaagaattcg tatccctttt
    24241 aggttggaac gaatggaaaa acctaaaatg gctaaaaaag cccaaataaa ttgcattaaa
    24301 gttaaagtat caaacggacg catcaataac cccacattga ccaaatttta agcattgaag
    24361 cacttcccgc tacgattcct actgtaataa atagtgcttc tactgctctg gcagttccag
    24421 caacaaggtc tccactcatt gtatcacgaa tagcatttgt aatagatagc cctggaacaa
    24481 gtaacattat tccaccaatg attatattat ctaaacgaat atagggtagg aagatatcca
    24541 atgtttttgc caacataaca atcaacgctg ccgctatggc attcagcaga aagttattaa
    24601 aagccaattt ctccagatat tttaagccaa aagtaacaac tactccaatt aaaaaagcaa
    24661 cagcaaattc agcccaggaa ccgccaaaaa gcaagcagaa aaatccactt gtgacacctc
    24721 cacagaggtc tcttatccaa gggctccaag ggttttcatt atctattttt ttcagttcgc
    24781 tattaaaatt aaccaattgt tcctgcaatt cctgttcgga aagcgcttta acttccatac
    24841 aattcaccat tgtgctgatg cgagttattt tccccaggtc tatacgccga ttttctattc
    24901 ttttaatact ggtaaaaata tttccgtttt catctttgat gctgataaaa atgccggtag
    24961 gagttacata cgattctgtt tcaggatagc caaaacagtt gcagaccttt ttcatcatca
    25021 attccacgcg gtttgtagtt cctccgcttt gtaaaattat gcgtccgact tctaaagcta
    25081 attcaagcac ctgttgagaa ctaactttgg gcatttttct ccttgggcag ttgttgtaaa
    25141 atttcagctg gcaaacctaa aattccggca atacgaattg cacatttagg aggtgctgtc
    25201 ttttcagaaa caggtaaaag agaataatcc attgcttccg aaagaagtgc caagcgtgtt
    25261 tccaaattgc tgtcctgcat tttttccaaa cgcttaagac cttgctcatc aattcctttg
    25321 atagtaaaat gagccaaacc tgcaagttct gccggagcac tgaaatgagt agcagaaatg
    25381 atagtattat tactatttat caaatactta agcgttgcta cacaaattgc ttcaccttct
    25441 tcgggattgg ttcctttagc aaattcatcc agtaaaacaa gtttatttcc cttacgcagt
    25501 aacatattag ataaagagac aacttcccgg gcaaaagaac ttaaggtctc actgttttct
    25561 cccgcatcgt gattgtaata aacctcatca tatagaggta atttagcttt tttagcaggc
    25621 aaaggtattc ccagtgaagc caaaatgcac atttggccaa tagttattaa agcagtgctt
    25681 ttgccaccca tattaggacc tgttatcaga ttatcctttt tgctcattgc aatatttagg
    25741 ggttggtatt gacgttgcaa tgtttccaga aaaagtttca gaggcaaatt aaccgcttcg
    25801 gtaagataaa taccttcaaa agaaggagtt atatttggca ggcaacagtt ataatgcaaa
    25861 gcaaatttag ctaagcaaaa atcccatgca tattctccgg cactgttata ggcaagctca
    25921 aaattactaa aggcattttg tatttgagtt gtaagagagc ataaaatctt attttcctct
    25981 tccgtcagct caattgagag ttgatgcagt tcggctttca aatcagcagt ttctttagaa
    26041 tcagccaggc ggaaattata atttgccaac ccttccgaga ccacaataaa atatttggat
    26101 gctaaaattg cctccagttg ttctttacga ttgcgggaaa gagtaaattc ctctttcaaa
    26161 gtaggcatac ccaattcctg ttttgccttt tccaaatccc gtttccgagc ttctttcaag
    26221 cgtttggcta tagcaaactg cttttgggta agctttttta agacagaaga ataggaagga
    26281 taaatccgaa aggtaggaag tttattgcct tcgggatcaa gcagggaaaa cattttttgc
    26341 atatccggta acggatgttg gtgattaagt ttatgttcct gtaatatttc ctgcaaatgc
    26401 aaataactga aaaggaatga ttttatctca aaaagttcat ttagttccaa acgctcggtg
    26461 gtctttaaat gctttatggg caaaagggaa agttcactaa aaatactttc taaccttgct
    26521 tccaatgctc tactttcttt gattgccttt aataaatttt ctgtattccg gttttgagtt
    26581 ttcaattcct caaagctgta gtgcttttta ttcagttttt ggtgtttaac gctttccata
    26641 acttcgccat tggtttccag caaggcaaaa agtttatcta ataacagcgc ttctttcaca
    26701 tttttttgca ttatttcatc cttataaatg cattttatat tagttacttc tgctgtcaat
    26761 catttttctg atagttccgt ttcccgtgat gaggattttg gtttgctgtt tttgtaagcg
    26821 ctttattgtt tcgggatgag gaaagtgata aatatttctg ctggaggcag ttatccaaac
    26881 ttcttgaggg ttaactgcct gtaaaaattc ggacgaactg gaattgcggg aaccatgatg
    26941 tgggattttt aaaaagtccg ctttcagttc ttgcgcatag ttatctacca gataattttc
    27001 agcttcggct tcaatatccc ctgtaaaaag aaggcgttcc ttttttgtat ccaaacggca
    27061 aaccagagaa gagttattct cgtttaaagg atgataggat ttatccggat gcaggaattt
    27121 caattttgcc ttgcctaaaa agaatgaaca ggtatcagta actgtatgta tttgagctgt
    27181 tccaaaatat ttctgtttag atgcatagtt ccagagctct tgtttggtgc tttcatcgct
    27241 gacaaaaata tgctttattt ttttatgatt cagcagtgtt atcaaacctc cacaatgatc
    27301 gctatgtaaa tgcgtaagaa caagatagtc aattttggtg atgccttttt tgcccagcca
    27361 aggaagtaaa ttcttattta accaactttc ttcggaataa tcacttttag ttaaaatatt
    27421 ttccgcaaac cctttctttt ttgtgccacc tgtatcaatc agcattgttt caccatttgc
    27481 caaacgaatt agagtgcaat cacctacacc gcaattaaaa atataaacac ttgcctcagg
    27541 tttttgccaa gtaggaatca gcataaaacc ggtaaccaac agagacatag gaattacggc
    27601 ttgcaaagca aatttgaatt taccgcgaat aaggaaaaaa ccccacagaa ttataactgc
    27661 caaagctatt gcttgaaaca gagataagta attggcagaa agggaaaaag gcaagttgta
    27721 acaaaactgc atccagcgta tccataaaat attaactgct ctataaacta taacaaatat
    27781 ttttgttaaa taccagctct gaggagttat cagcactaaa aaagatagcg ggagcaatat
    27841 tccgctaagg ggaataccta atgtattgcc aattatacca tttagagatg cagtcccaaa
    27901 ataatagaga gtgaaaggag ttattgctat ggaaataagc gccgaaagta aaataccatc
    27961 ttgtaaatca ttaagcactt tataaaaggg attgttcaat attttatccc gggaaaataa
    28021 ccgaaaattg ggcaatccgt aaaaaataac cagcaccgaa acaaaggaaa gctgaagtcc
    28081 tatattaaac aactgcaaag gacttaagag agttataata aacaaagata acgccaatgt
    28141 ttgagctccc gaaacaggac tttgcagcca gcgagctaaa atacctactg aaatcataat
    28201 aatagaacga gttacagaag gagaccagtt attgagtgct gcataaagta aaattaacgg
    28261 tagaaaaaga aactccgcta tttccctttt aaaaaacatc cttaacagcg aaaccataat
    28321 caaataaata aaccagatat gcattccgct tacaactatt aaatgtataa ttcctgctcc
    28381 ggtaagctga gtaatgtatt cctgctttaa tcccgattca ccaaaaagca atgcttttgc
    28441 ccaatccgca tcttctccca atttagcttc cagtgaactt agcagttcat aacgcaatct
    28501 gccaattata gcgctaactt ttaggttggg aaattcctgt gctttgccca attgataagc
    28561 aactgcagaa taacgattag gataaatatc caaaatagga tcgcgggtta agggataaat
    28621 ttccgctaat aaacgataag ttctgcctgg gattaagctt tcattggtag ataggataat
    28681 tttttcttgt agaggataag aggcaaggga atcaagctct atggcataac gcttttcctc
    28741 cactgaaaag acccttgtaa cattaaaagt tatttcctgc tgaaggtgtt ctttttcctg
    28801 taataatatc tctagttgag aactttcctg ttggcataaa gaaaagcgta acatacctaa
    28861 aaacaaaaaa agtaacagca gcaaaatttg gcgtatctta cgcataaaga tagcaacaat
    28921 tgttaatcca ccggctacaa ttaattgaaa atatattccc ggcaggaaac attttgccag
    28981 gatgataccg atagtccaaa acaaaaccgg caagagaaaa ggagcaggtg cttttctgtt
    29041 tgcctccatt tacattttat ctccatcttc aggatttttt atttaagtca ggagtcatta
    29101 gcgctttccc aattttatct cttacaatat cttcattggc gcttatgact aatgacgacg
    29161 ataaattatt ctctcacaga tttcatagat gacacagatt tataattttt tatccttccc
    29221 ttataaacaa aaatttatcc taactgccat ttgatattca ttcttccttc ctaatatcac
    29281 gcgttttcaa tgccaaattc atttgtaagt agcgtaaaag atattgcggc agtgaactac
    29341 gaaaatgtcg ttcttccagg ttaaggtata tgtcttgtat agagcgctat ttccacttta
    29401 taaggtcttt ccccttcttg taaagctcga tttagtttat ccagcatttt agtccgtttg
    29461 cttatactat ttgccttcac tttcacacta aataaagcgc tggcaatttc ttcgccttcc
    29521 acatcccaaa cccttgccat taatgcaatt tcctggtctc ttttaccggt attggcaaga
    29581 gtgtattcca gttctataaa ttgagtgccg aaattggaga tgcggacatc gctgatttga
    29641 attcgggacg catttcttct tacaccggag cgttgaatga aaatccccag aataacaaga
    29701 acaataacaa taatcaggat aaactgtttc ggtttatacc gcttcgggaa ttcaagccga
    29761 tcaactactc tcatttttga acccttaata aataaactgc caagcagaat agaaggtatt
    29821 ttgccagtca tcaaggtgag aagtttcaaa accaaaaagg agacggtgat gagcaaaaat
    29881 atctataagg acattacttt tcaaggtaat agtttctgtc tttgttcctt gaaaccaatc
    29941 cgccgtgcct tcatcaattt cttccgctgt attaaaaaca ttatgataat tttgcctcca
    30001 gtcactgcca aaacttccct gtttgcaata agatacgcct ccctgccaaa gaaaaatatc
    30061 tttatatggc aaacgcaaaa gacaagaaac atctaccaaa ttggaacctt taggatagcc
    30121 cagaggtctg ccatcagtag aaaacatcgt atgattcaaa tagtgcgtat aagtgaaagg
    30181 acggacagct gtaatttctg ttgttatatc tgcagaagga ctttccggta aagaaaaagg
    30241 aacattccaa cgcattccac cctgtaaagc atatttattt ccccaccaat cactgaaaag
    30301 tttgctggaa ctaaattcat ctataagaac ttgtgcataa agaaataacg gttgaacagg
    30361 acgataagaa cctccggcaa aaatcattac attatctcta tcccaaaggt tatgctcaat
    30421 tgctcgccag aaagcattag gtaaaaagta gctaatttcc ggtgaacgat tgccatagat
    30481 gatttcttca ccaaggaaaa gatgcaaatt agaacaagga tgaaaaccga attgatgcaa
    30541 tgccagatac ttatcgggat aatttttgtt gttgtaaaaa tggtcattat atatattgta
    30601 agtgctatct gccattaaag atgcgtgtaa aaaagagata ctaaaaatgc ctgctctgcc
    30661 ttctgccaaa atataaccgt aatcattcac cccatcattc aaaatgatac ttccgctaat
    30721 agaattgcct acgggaaatt ttcctcgtcc taaagccaca gttaagttct tggcattgta
    30781 gctgatatct ccgctaagat tatcaatgtt cagtttttca tcggtggtat tgcagtaacc
    30841 atccaccagc caggaagttc ttgctgcgtt ttgattacct aaaaaaattc cgttccacca
    30901 tttagtattc atttctatgt gttttccata ttgcgatttc aactgccaac ctctatagag
    30961 aaagctgtaa ctttcatcct tttttacagg ttccaaaccc ccaataaaat tcagttcccc
    31021 tcgcaattta taatcaggac tttgataact caatatagaa taattggtat ttcccaaacg
    31081 acaaatatca agaacagcgt cacctacgct ggaacgcgag acaaaaggaa ggtataatcc
    31141 attattataa atagagatac tatcttcttt agcgcgttgg ggataactta ataaactggg
    31201 ttttaaccct tgctgaatgt ccggagcaaa ggcagaatcc ggtatcaaac gcaaagcagg
    31261 taataatgaa caaaaaagta acaggatagc caaacatatt ttcttagcca tattttattg
    31321 ggattcatcc tgattacaca attgcaccag gcgttccact tcctcctcgg atatttttaa
    31381 ttctttagca atttcactat ttgtccagcc aaaatcaagc aaacgacgaa ccataagaca
    31441 gagggtatta ttttcctcgg ggtgaatatc ttctttattt tcgggttcaa tattgatgtt
    31501 cagtgttgtt ttccttttct ttctgctaat gcttgcaatg aaatgatata ttaaaattaa
    31561 cagcaacatc aggatgatta acagcataat tctgctaatc cattttctgc catataattt
    31621 tttaacttta gcgggtttag ttgtctgctt cttatcctgc tgagttttgg gcataatata
    31681 tagggagtcg gtcgttgttt tttcttcaac ggaacttatt ttgcaggcat tttgagcttt
    31741 ctgcggggaa cataattggg ctgaatcttt tttctcagtc ccctttagca tttgttgcgc
    31801 taaagaataa tactcggatt tttcaggaat tttccccact acttccttta gccgaccgct
    31861 acctctttgt tttaataaac aagcccattt atacaatatc tcataatcac ttggataatt
    31921 acggtgaagt tcggagtaaa ttttatcagc gctattcaat tttcccacag aggtatagaa
    31981 atccgcaagc tctatttttt gttgtttatc cggttgagca acaataagat ccaaaactaa
    32041 acgccggggt gcatccaatt ccatctgttc caaacagtat ggacagaaag tagagataac
    32101 tattttatcc ccaaattgtt ctatactttt tatcaaaccc tcagcagaat tggaaatatc
    32161 tactttactg acatcggcat tatctaactt tatttctaca aagtcattac ttttatcaac
    32221 tttataatcc cctgtgccat aaagacctat tacgattctt gtagccgctg aaccgtaatt
    32281 ctgccaatca atataagtaa cctccagagc agaacaaaat actgccgtca gtaaaataag
    32341 cgtaactaaa aatctcttag tcatttatgc atctctttgc tttttctgaa aagaaagtgg
    32401 caaattccgc tttgcccctt aaatttcagc actttaaaag gtatgctgaa aagcacttag
    32461 cgccatcttt tgctatcctc tttgctatca tcattttagc atagcaggat attaattcct
    32521 gataacttct tcctttttct aacttatatt agtatttcac tctttttcaa ctttataaaa
    32581 ttggcaagtt atttttttcg ctggggctgt ccctaaacaa tgatagcgat gatggcggaa
    32641 gccactcgtt tgaatggaga attaagaatg aattaattca ggagtcatta tcgcttgtat
    32701 aactttgtac attgtcacta attaatgtag cgatgaggac tcataacgac cagagataat
    32761 tttataactc tttggcaaat tggaaattgt caatcctttt tttctcaact gcataatatg
    32821 gtttaaacac tatagaattt actgctccag agaaagttat caaaccgcag ataactcctg
    32881 ccttactttg gcttgttttt atctttgtgt tacttcttac aacaaagtaa caaccaagta
    32941 acgattccct aacgagacaa gggaagtgtt acggaagtgt tacggagtcg ttacttaatc
    33001 gtctatataa caagcaatta tgcattctaa cacaataacg acaagaaata tggctaattg
    33061 ttttattatc aatggataag acaatattac aaagaattat acaggtagtg tctcaaaggt
    33121 tggtgtaaat aaggtgcgcc tgatgaagaa aaaggttgag ccggatcccg cactttgttt
    33181 tgatggtttc aacaaaacta aaagacaagg agaagatcat aactcaaccg aatagaaaga
    33241 aagatgaaag agcggaagta gaatcaaatg caccaactca ctggagcgct taaatgaaga
    33301 gctcaagctc agagagaaat gtttcaggat cttcctggat gaaaagagtt gcctgcggct
    33361 gtttggtgcg atcctgcata gcttttcaga ggattggata agcggtaaat tgtatctttc
    33421 ggagctaata gaaagaatca gagaaaacca gaaaagggct atatcatttg aggcaacgca
    33481 gcaaggtagc ttgttttgaa aaagaacttg acttggattt ataagatgaa aagaatgcaa
    33541 ttggtcgctt ggcctaatat agattgtgag taatgatgat agttagaatg tactcaaaga
    33601 acctgagaaa caacgagttg gaatttacac caacatttag gactcaacta aataaattca
    33661 gtgttataaa ttaaattagg agataagcta tgataaaatc agtagtaatg caaaactttg
    33721 gtcctttggt tgatactaaa tgggaaaatt tgggaaagat caatgttatt attggagcca
    33781 attcttcagg taagacattt tatttaaaag cattatacac tgctatcaaa tccctggagg
    33841 gatataaaag aggtaatgag aacaggtctt tagcggaaat tttggcagat aaattatact
    33901 ggacttttca agttcccaaa atcggggata ttgttacaaa aggtcaaccc gatccgcttt
    33961 cctttgaatt ttcggttggt aataaagatc tcagctatac ttttggtaag gataccacta
    34021 agaaaatatt atcccttgaa aactacatat atcctcaaaa aagtgattct atttttctac
    34081 ctgctaagga agttttatct atctatcaaa atatcataca atattatgac caatacaaaa
    34141 cttttgggtt tgatgaaact tatgttgatt tagttagagc tctaagcatt ccccgcataa
    34201 aaggaaagac atatcaaatt attgcaaaat caagaaaaaa acttggagaa ataatccaag
    34261 ggaaaataaa gtttgatact gattcccagg tatggcaata taaaaaaggg aatgttagat
    34321 accctattgg tgtaacggct gagggtataa agaaaatagg cattttagac actttattgg
    34381 ggaatagata cttaacccct caatcaacag tttttattga tgaaccggaa gctgccttac
    34441 acccttccgc agtttctcta tttttagaaa taatcagtga tttagctgaa ctgggaatgc
    34501 agttttttat ggcaacacat tcatattttg ttgttaaaaa actctatcta atcgctcagg
    34561 aaaagaaact aaacatacct attgcttctt atgaagataa taaatgggtt tatgatgatt
    34621 tgaggaatgg aatgcctgat aattcaatag tgaatgagac cattaaatta tataaggaag
    34681 aagttgatct ggtgctgaaa tgaataaaat aataattgaa gaatcaaata tgaaatttgg
    34741 accttatgat aaagaagccg tttttctgat agagcaaagc agggtatata aagagatagg
    34801 taataatgta aaaattgccg aatttgtttt ggagaaatca tcccagaata taaatattat
    34861 tgaagccaaa tcaagctctc cacaaccgca gaataatgaa gattatgata cctttatcag
    34921 aagcatttat gaaaaatttg ttaatacttt ttctcttacc ttagctttaa ttttaaaaag
    34981 gttcccttcc cattatgaag ttctgccaga aaggttagaa aaaatagagg tatcaaactt
    35041 taaatgctta tttgttttag taatttatgg acacaaagaa gaatggttac ttcccataca
    35101 ggattcctta aataaatctc tatcttcatt ttttaaaata tggaaccttc ctccaacttc
    35161 ggtaatagtc ataaatgaga aaatagccaa agctaagcat attatcaaat agctattttt
    35221 atgaatacgg attatcttaa cttggcaaag gcatttttgt ataaacagcg tttatggcaa
    35281 acctgtggaa ttgtttgtga atacggttta tcactgataa cccataaacc gctggtgcat
    35341 ttttatccac ctgccttaat gctggaacca acaaatattt gtaatttaca ctgtccttta
    35401 tgtttaaccg gcaatggaaa attgcttcgt cccaggggaa tgatgtcttt accgctattt
    35461 aaaaaaatct gtagggaagt tcaaaataaa attggaatgc tgattttatg gagtcaaggc
    35521 gaaccctttc tgaatcccga tttttatgca atgctggaat atgcctctaa acaaaatttt
    35581 tacactatga cctcaactaa tgccagcttg gaattagata ttgaacgcat tgtgaaaagc
    35641 ggtttgcata aaattattat ttcaatggat ggaattacag cagagaccta taataaatat
    35701 cggataaacg gaaactttga tctcgtgctg aaaaatatgc aggaactggt tcaccagaaa
    35761 gaaaaattgc atagcaacaa gccatatatc gtttggcagt ttttgattat gaaacataac
    35821 gaaaatgaga ttgaagaagt taaacacttg gcgagaaaac tgaaaattga taaactggaa
    35881 ttcaaaacgg ctcaaatata cagttctgat gaccttgctt ttttaccttc cgatcataaa
    35941 tacagtcgct atttccaaca gggcaataaa tttactctga aaaccaaact gaaaaatcgc
    36001 tgcatacgtt tatggacaca accggttatc aattgggacg gggaaatgaa tatctgctgc
    36061 tacgataaaa atggtagcat aaaaatcggc aatattcaag agcacagttt ttctgaactt
    36121 tggtgtggag aaaagatgca aaaaatgcgg aaggcaattt taaataatcg ttccgctttt
    36181 gaaatctgcc aaaattgcgg tgaaggaatt gtgcaaaaaa tcaaaatgga gaaaggtgaa
    36241 aggtgaaaaa gtggaaaggt ggataagtgg aatagtggaa aggtgaaaaa gtgaaaaggt
    36301 gaaacaagga gataatttta ttacaaaccc tgaattatgt agtcggagct cgctaaagtt
    36361 ccacattttg tgtagtcgga gctcgctgaa gctacacaaa atcaggaacc ttttttcttg
    36421 tcaaggaact gagtcctccg gctacaactt ctgacttttc acttccaaat cctctctttt
    36481 tctctttgca aaaaatcctc tctttttctc tttatctctt tgtaaaaaaa cctcttttcc
    36541 cttttgtaaa aaaccctctt ttcctcttta cctctttata aaaaatcctc tctttttctc
    36601 tttatctctt tgtaaaaaac cctcttttcc tcttttctca gaattataat aaacagatac
    36661 tgcttgacgg gataagcatt ttattttacc tgtttttgca tcagtaagat gaggtatcat
    36721 tatgaaaata agtgagataa tgaaacagat agaagaattt gctcctttgg gattagctct
    36781 ttcctgggat aattctggtt tacagatagg tgacttaaac agtgaagtgg aaaatgttta
    36841 cataactttg gatgtaacct caacagctgt tcaaaaagca ttagaaaacg aatgtcaatt
    36901 gattttaagc catcatcctt taattttcca acagcttaaa aatataacaa atcctcttta
    36961 tttggaaatc atcaaaaata acctttccgt aatttcccta cataccaacc tggatgttgc
    37021 ggaaaaaagt gtaaatcata gtttagccca aaaactcggt ttggaagtta ttggtttttt
    37081 aagttctgaa actggagcaa ctaactatat tatagtggtt tattgccctc ttgaagatgc
    37141 ggaaaaaata atgcattcag cttggagtgc aggtgccgga aaaataggaa actattacaa
    37201 ttgtgccaat aaacaggaat ccgaaggtta ttacaaagct tcggaaaatg ccaaacccta
    37261 taaagatagc ggtattgaag gtcatattgc ggaaacctgt cttcaattct gctgtgaaag
    37321 ctttcattta aatgatgtct taaatgcaat tcgtcaaaat catccttatg aaacaccgtt
    37381 gatttatcat tatgaggttg ctaatcccaa tccggcttat ggattgggtt tgatttgcaa
    37441 atacaacgct gaaataaatc ttcaggactt ggctatttta gttaaagaac gcttaaattg
    37501 tcctcaggtt aaactttgga cagccagttt tgacctgcat aaaaatatta acaggatagc
    37561 tatttgtgga ggtagtggca gttctctgat ttcagccgct caaaatagtg ccgatgtttt
    37621 aattaccgga gatatttcct atcacagctt tctggaaagc aaaattcctc ttattgatgc
    37681 ggggcatttt tatacagaat atccagcttt gcagttactt gcggattttg ttaacaagtt
    37741 gcaactgcac ccttttttaa tccctcaaaa tgaacattta tatacccaaa accttctaat
    37801 tatttaaaat agcagtaaca aacaaggtga gaacaagatg caggaaatca agcgtaaaaa
    37861 tacaaggcgt attcaattag gaaatatccc tataggtgga ggtgcacctg taagcattca
    37921 atcaatgctt tcagtgaaaa cctccgaccg ggaaaatgcc attcaacaaa ttcaggaact
    37981 ggaatccgtt ggttgtgaca taatccgttg tgcagtaatg gatttagatg atgccaaggc
    38041 aattaaagac ctgaaaaaaa ttgcccaagc tcctttagta gcagatattc attttaatta
    38101 ccgtttagcc cttgcagcta tggaaaatgg tattgacgga ctgcgtatca atcctggaaa
    38161 tataggaaaa attgaaggtg tgaaagcatt ggttgcttca gcaaaagaac gaaaagttcc
    38221 tattcgcatt ggagtaaata gtggttctct acctaaggat ttactgcaaa aatatggagt
    38281 tagcgctaag gcaatggtgg aatcggcttt acggcatata agaattttgg aagagctttc
    38341 ttactatgag attaaggttt ccgtgaaggc atcctcaatt cctttaacat tggaaagtta
    38401 ccgcctttta agcagtgttt gtgattatcc gttacatctt ggaattaccg aagcaggaac
    38461 tgtttttcgg ggttccataa aaagttccat tgctttagga atccttttag atgaagggat
    38521 tggagatacg ataagggttt ctttaacgga taagccggtt caggaagtga aagttgccaa
    38581 agaaatattg acctctttgg gtttacgcaa aggattaaat attatatcct gtcctacctg
    38641 cggacgaaca agaatagacc tgattaaatt agctaacgaa gtggaaaaag cacttgagcc
    38701 ctataacaac tatcctttaa ctattgctgt tatgggttgt gctgtaaacg gacccggaga
    38761 agcaaaagag gcagattttg gcattgcagg aggcatcaaa gaaggattgc tttttgcaga
    38821 aggcaaggtc ttgaaaaaag ttccagaggc atttttagta aatgaacttg tggctttagt
    38881 gcaggattat atcagtaaac attgaagatg cttcttaaaa tattcttcac tttcttcaaa
    38941 ataggcgctt ttaccattgg cggtgcttat gcaatgattc ctttaattaa aagagaggtc
    39001 tgccaaaaaa ataactggct taccgaagat gaattttggg atggactttc tgcggcacaa
    39061 agctgtccgg gacctatagc catcaactta agtatttata ttggctacca tctggcaaaa
    39121 ggtaaaggaa tggcaattgc tgttttagct acaattctgc cgtcttttat cattattctt
    39181 attattgcta tgctgtttca acagtttgcc gaaattactt taattcaaaa ggcatttcat
    39241 tccttgcgtc ctgcagttgt agctttaata gcggttccct taattcaaat gagcagacaa
    39301 agcggattag ctctatccaa tttttggttt cctcttagcg ttgctatttt agttggtttt
    39361 ttcaggataa gtcctgttta tttgattcta atcactattg cctttgctgt ttatcaggga
    39421 ttaaaaaagc aaaaattatg atttattttc agctgttcct gacttttttc aagattgggc
    39481 ttttcagttt tggtggcgga tatgccattt tggcaatgat aaatcaagaa gtagtattaa
    39541 aaaatgcctg gattacccat tcccagttta tggatattgt agctatttca caaatgacac
    39601 cgggacctat agctatcaat gcagctactt tcatcggtta tcaaaaagga ggttttgtag
    39661 gttctgttct ttctacttta ggagttattt ccccttcgct gattattatg actttcataa
    39721 cgttaaccta tatcaagctt aaaaatcaac cctggttcaa gaatattttt cagaaactgc
    39781 gtttgttatc cttgggatta attgccgccg cttttataat ggttgcagga gatgcttttc
    39841 gtgatctttt ctccgttttg gtatttactg catcttttat agcgagctgg aaatttaagc
    39901 tcaatccttt ctatttgctt atcggagcag ctattgtagg catagcattc gggtaaaaag
    39961 gaagaatgaa tatacttcta tagtaacgaa tatattataa atccctttat ttatgttcaa
    40021 ttatacctag tgtcaaataa aatccgcgaa atccgcgtgc tattttcatt tcaaactccc
    40081 caaatatctt ttgcatatcc ttttatcgtt tcatcggaag aaaattttcc catattggca
    40141 atattggcta aagacattct attccattgt tgtttatctt gatagacctt tacggcttca
    40201 gtattaactc ttataaaatc aggcagatca gcaattagac aatatttatc tccctgatat
    40261 agaagcaaat ccgttaaggg agtaaaaagt ccgggattta aaggattcag tatatcggac
    40321 tcaataaaat caaaaatgcg tttaagatct tcattttgac gcatcatttc gtaagggtgg
    40381 taaccgcttt cttttaaatt tttcacttcg gcagcggtca ttccgaagag gaagaaattt
    40441 tcttctccga ctgcttcccg aatttctata ttggcaccat ccaaagttcc aatagtaaga
    40501 gctccattga gagcaaattt catatttcct gttccggaag cttcagttcc agcaagagaa
    40561 atttgacggg aaatttctgc cgggggcata attctttctg ccaaagttac gcgataattg
    40621 ggtaaaaaga taacacttaa gtaacgggaa agctctttat ccttttctat ataatcactt
    40681 aaggcacaaa taaaacgaat aatcaatttg gcaataaagt agccaggagc cgcttttccc
    40741 gcaaagaaga aactttgagg ataaggggtt tttccatccc gaatttgatt tatgagatgg
    40801 ataataccca aggcattcaa gtgctggcgt ttatattcat gaatgcgttt tgcctgaaag
    40861 tcaaagatgg attcgggatt cagcttgcgt ttatgcaggt cttcgtaata cttgcagaat
    40921 tccagtttat tttgcctttt aacttctccc aggtcttcca aaaaggtact atcattctta
    40981 tatttattca atttggcaag ttctttaagg tctgttttcc aggcagagcc gattgcctga
    41041 gtaatcaatt cacttaaacg cggattgcat agcattaacc atctgcgagg tgttatacca
    41101 ttggttttac tgttaaattt atcttgccag agagcataaa aatccttaaa aatcagatgt
    41161 ttcaaaatct ccgtatgcag ttccgaaaca ccgtttacac tatgcgaacc aactatagcc
    41221 aaatttgcca tacgaatgct ttttacgggt tcttcttcaa tcaaggaaac ccgactaata
    41281 aaatcgtcat cgtataaacc tgtagctctt aaagaagaaa gaaagcagtg attaatttca
    41341 taaataatct gcaggtgacg cggcaataac ttttcaaaga gaagaacctg ccatttttcc
    41401 agcgcttcag gcaaaattgt atgattcgta aaagctatac atttttgagt aatttcccag
    41461 gcaatttccc agggcaggtt tctttcatcc actaaaattc tcatcaattc agcaacagca
    41521 atagcaggat gagtatcatt aagctgaatg gcagcttttt caggaagttt acgcaagtca
    41581 ctttcctttt ttagaaaccg gcgtaaaata tcctgcaaag tagcgctgac aaaaaaatac
    41641 tcctgcctaa accgtaattc cttaccctgc attttgttat cattaggata gagcactttg
    41701 gcaatcattt cgctatgatt cttgtctgcc actgcttgca tataatcacc ttcattaaaa
    41761 tatgccaaat taaattcacg cgtactttta gcgctccata agcgtaaggt attcacatag
    41821 ttgttttgaa acccaggcac taaatagtca taagccatag ccattaccgt ttcttcagga
    41881 atccaacgca tttttttact gccatcttca agaatttctt ctttcactat gccaccaaaa
    41941 taaacaggaa aaagacgagc aggacgcgca atttcccaaa cagaaccata acgcaaccag
    42001 ttatcgggtg tttcaacttg ttcaccatta ataatatgtt gaaagaaaat accatattca
    42061 taacggatac catagccata agcaggaatt ttcaaagttg ccatagaatc taaaaaacag
    42121 gcagcaagac gacccaaacc tccatttccc aaaccggcat cccattccaa ttcgctaagc
    42181 aaagaaagat caaatccgtt ttcggtaagt gctttaatgc tttgcttttg aatatcaaga
    42241 ttgagaatgg cattgcctaa agagcgacca attaaatatt ccagcgataa ataataaacc
    42301 cttttccgtt gcagagcata ttcctggggt tgagttatga gccaacgatc catcagaata
    42361 tccttaactg ccgaggcaaa actaacataa caatcataag gagttgctga aaattcgtct
    42421 ttgcccaaat caaatttgag atgctctata aaacgatgca ctatttcttc agcagttccc
    42481 tcaatactaa gatgagaaag cttttcgcaa aatgccttat tcataatttt ttcctcctct
    42541 aatgtcaaag cttataagac aaaataagca atttaacaat tttggcaaat agaactgtag
    42601 aatagctata ttaacctaaa aaatacagtt agtaaaaaat gggttggcaa ctcagagaat
    42661 tggtttatat tatattcagt gaattgaaga aattaaaaat tctgcctgca aggaatgatt
    42721 tctaattgca attaaggaga tataatgagg aatatgaaag aagaaaaaaa atcgtttcca
    42781 tcttcaaatg caactgaaac aaattacttt cattttgatt ttatatagat gaaaacaata
    42841 gatatatttc atagttgaga ggaacaatga tcatttttaa tatttattcc gcattcaaat
    42901 cagttagttc tactatgatg agcgtttctt acctggtgca aaaaagcaca aaaagcaagt
    42961 tataacataa aaggtcaaaa ggttcgctgt ctggtgcagg aaaatttccc ttgcggtgag
    43021 tataaaatta tctgggacgg tttgaatgac cacggaacta ccgtttcttc cggggtctat
    43081 tttgctgaaa tgagcacacc cggatatcgt aaacaaatta aattattact tactaaatga
    43141 agatttttcc aggaggaaaa atgaaaatcc gaattttaat cctatccgta tgtatcttaa
    43201 tgctttcctc tgtgctactg gcacaggaga tggttcctct ttccaatttg aaagagcaga
    43261 aaattgaatt taaaagtgtt ccgctatcca gcaacaggac agttccccaa tttactttca
    43321 cccgaactcc tatttcttta cttacttctt attacgacta tatgatagga ggttacaacg
    43381 gaatgccagt tcaggtgatt ccggataatg ttactggtgg tggcggttat tttttaactt
    43441 atcatgccag gcgaactgct acaggaactc gtcgtatatt ttacgcttat cttaatgctg
    43501 atatggatgt tatctttaat attgaaatta caaatgtaac aaataatgaa ggatatccta
    43561 ctgtagcggt agaccctgtt tccggaaaac ctttctatgc ctggcattgt aatactgatg
    43621 ccgatacaca actggaggtt cagcttacat ctgatgcttt ttatgaaggt attttcggac
    43681 tgtttaacga ctatataacg cttattgata atcctataac ggtctcaggt acaactgaca
    43741 acgaatttat atggcccaca gcagttatcg gaccttctcc ctttgctggc aaaagacgaa
    43801 tttatgtagt tgccaaaaac tctgtaactc acagttacgg tccctccgaa aatcccagaa
    43861 ttgcctgggc agatttcaac ggaaatgaca ttgaaatggg ttatcctctt ttatggaatt
    43921 atacttccat tccggaattg gaccaatgga atatagatat tcaatggcgt cgtccttttt
    43981 ttgccattac ctgtgataat ataggaaatc tctattatgc gggatttcat tttgctaagg
    44041 aagctgatgg ttcaactgat atccttgagc cggatttaga tgtttttaaa tgtgataatt
    44101 acggaaccgg aacctgggtt catcttagta agtggagcaa tttaccttcc tggaatccgg
    44161 atatcagtcc tacaaataat aatggttatt ttacagatac aaataatatt ccttatgaag
    44221 ataacgaact tttttggaaa gtaatggctg caaattctgg tcatctgaat gccattgtgg
    44281 atgaccttgg tagaattcat tatcctgctc tttggggatt gaataatgct gatggctatt
    44341 tttatccggc tatgcaatat cccaaagaag tgatctttga tccttctcaa ccaacagatt
    44401 cgcagttcag aataaatgaa atctggcctc aaaaaaaccc ggctaacact tttgatgaat
    44461 attaccagcc atgggataat gaacccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44581 nnnnnnnnca ataatatgaa aataactgaa ggcaacggac acggaatgtt agcggttgtc
    44641 tggcaaaatt gcaggagagc taaacaatat aatgcaaatg gtgataccga ttattccgct
    44701 tgggcaaata cgcctgaaat ctggatttcc gtttcttccg ataacggaga tcactggagt
    44761 gagcccataa aactgaataa tattgaaaca cctgaatttg ccggattaaa accaatgtgg
    44821 gtttatcctg ccaacaaagt aaaatatgta ggaatgcagg gtaaccaaaa aataggtaaa
    44881 ttgggcataa tgttttacaa cgattttact tggggtgctt atgttatcag ccctggagtt
    44941 ggtagtacaa atgatggcgg acaagtgatg ttcaccgaat tgcaaattgt ttttcctcct
    45001 gaaggacaag ctcaagaccc ttttggacag ccaattattc tcagcgggag tatgagtgtt
    45061 atggctggag tgcatattaa cggacaaatg gcttcggcag gagatgtagt agctgcctat
    45121 gttaatgtaa atggaagtcc ccaattacgg ggcaaggaaa ccgttcagat taatgataat
    45181 attgccggtt gcttgctaca ggtttacaca gaaaccaatg gagaaacggt gtatttcaag
    45241 gtctgggatg ccagtaccaa tcaggtctat aatgttacag aaaccctttc cagtcaagta
    45301 aatggggtaa ttggcagctg gcctgataat cttttctggc taaatgcaac tttaactcag
    45361 cagcaaaata tccctctaaa tacgggctgg aatatggttt cgctcaatgt tcatccaaac
    45421 agtatggcaa ttacggatat ctttgccaat ataatggcga acattgtaat gatcaagtca
    45481 ccggatggag tatttattcc tggcaatcct tataatactt taacccagtt agcggatggc
    45541 aaaggtatat ttgtaaaagt aagcaatcct tgtgtattat ccgtaaccgg aatgccgata
    45601 gatataacag ctcctatcta tttgaatgcg ggttggagtt taattggtta tattctccct
    45661 attcctttga atatcagttc tgctttgata agtttaaccg gcaattatat tcaagtgaaa
    45721 ggagtggaag gcgtttttga gccgtataat cccttcagta ctctttctgt tttaagtccc
    45781 ggaagagcat actggattaa attgcaaaat ccaggtgttc tattctatgc ttatcctctt
    45841 aaagaagaac aaagtccgca aaatattctt tcctgtacca aatggggaac accgatgcaa
    45901 aagacaaaca gtcaagcggt cttatgttct ttaggtagtt ttgcttctga aggagatcaa
    45961 ctggcagctt tagttcaggg agaattgaga ggattagctc ctttaataaa acacaatagc
    46021 attttaggaa cttatatcaa tatctttacc gatagtcctg gagaaacaat tgaattcaag
    46081 cttatccgga aaaacagtgg agaaattata gatttacaac ccaatttgag cagtgaaccg
    46141 ggagagacaa caggtgacta tggtaatgga atattttata ctttaaccca agctccgaat
    46201 caaataccgg aaattgttac agaacttgtt tctgcctatc ccaatccttt ttcagtttcc
    46261 acttccatta ccctcaatgt aggcaaagaa aaacagcccg ttcaaataga tatttacaat
    46321 ttacgggggc agaaaattac taccttactg caggataaac tggaatttgg acagcataat
    46381 attgtttgga acggaactga tgaaaatggc agaagagtgg cttccggcgt ctatttatgc
    46441 aaaatgcatt cagaaaataa aactcaaagc atcaaactaa tgatcctgaa atagaaaaag
    46501 gagggataat gattcacaaa attattcccc ttctgctcct tactttattc gcaggagcag
    46561 ctttagctat tgacccttgg ggtgaaccgg aaattttacc cggcagtatg accgttatgg
    46621 ctcaggtaag tatatctgga agccctgcag ttaataatga tgttttagct gcctttgtaa
    46681 atgtaaatgg gacacttcaa ttacggggca aagcatttat tcaggtgttt ggaggtgttt
    46741 ccggatgtct tattcagatt tacactgaaa ataatggcga aataataacc ttcaaagttt
    46801 gggatgaaag tatccagaca gtttttgaag ctaatcaaac cttggtttct gaagttaata
    46861 gtatagtagg aagttaccct gataatatgt atcaaataga agcaggacaa acaactgtaa
    46921 ccgatccctg gctggaacct gtaattctga caagtagtat gacggttatg gctaaaattg
    46981 gcatttccgg agttcctgcc actaatgaag atctcctggc tgcttttgta actgttgatg
    47041 gtatagaaca attacgggga aaaggtaata tctctgttat caatggcatt gccggttgcc
    47101 tactgcaaat atacacagaa gttaatggtg aaactatcta ttttaaggtt tgggattata
    47161 gtgctcaaca aattgttaat gcttttccta ctctaaacag tgaagtaaac ggaatagtcg
    47221 gttcctggcc caataatctg ttccatattt atgcaggaaa tgtgcaaacg gttgctacac
    47281 ctgcattttt acccttggga ggaacctatc aaactgctca aaatgtaact ctcacttgtg
    47341 caacttccgg agctcaaatt cgttacactt taaatggaac ggaccctaca gaaacttctg
    47401 ctctctatac caatcctatt catctgaatt tgaacagcac taccgaaatt agagctaagg
    47461 catttaaaga gtattggata cccagtaata ttgtaagtaa tacctatatc attactggaa
    47521 cagttccagc tccttctttt aatcctcccg caggttcata tctcaatgcc atagatgtca
    47581 ttatcagttg tgctcttgag gaaacagtta ttcgttatac tctggacgaa acagaaccca
    47641 atgagaattc aatgcttttt actgctccaa tacatataaa ccaggataca atgcttaaag
    47701 ctaaggcata taaaactgac tggctaccca gtaatgttgt ttccgcaatt tatcaaattt
    47761 cttccgctaa cgaagataac ttgaatgctc ctctggttac cggtattcaa aatgtttatc
    47821 ccaatccttt caccggttgc actacaattg aaattgccat caaagaatat cctcaaaact
    47881 atctcctgaa aattttcaat attaaaggag aacaagtgcg tcagtttaat ggcagtgcta
    47941 aaggaatcat cactcagaac tgggatggaa cagataacaa aggcaataaa ttagccgcgg
    48001 gagtttatct cctttatttt caagttggtt ccaaccatta tacccgcaag ctcatcttac
    48061 aataaatatc tattcaattg acggggactg aaaaataagg tccccgtcag tttcttctct
    48121 cggaaaaacg atatcctcgt ttttttaaaa tcagaagtcg ttactgctga aataactttt
    48181 aagctatctg tgaactttta tggcagtgag aattaatgac atcgttaatt tattatttta
    48241 gcgttggttt caataagtta ttgctcaagg tcgtgatgag ttatatacac cgttaaggat
    48301 atcttaaaga ataaagtatc gttggcgcaa atgactcctg acttttaaag acatcaacct
    48361 tccgggttca attttatgct ttaaaaatcg tggttcttac cctcattact ctatcattgc
    48421 tcacccatta ctaatgccat ccgtaatggg taagcaatgg gtaagcaatg ctattaagga
    48481 agaagtagta actatctttt aatcaggtag ttaccaatat agtatgaaac actataggaa
    48541 taatggattc atttagaaga aaatagaacc cagataactg gatagaggga ttgagcagga
    48601 tttttttatc ctaaatccat attcataaat cataaataac agaaaaaatt ccataggaat
    48661 ggcagcagca gatgagggtt gacaaaatga agcactacag aaattggttt tattattcaa
    48721 agtaaaggaa atataaaagt caattaagca caggagaaga gaaatgaaaa tagataaaaa
    48781 ccttgatata tggaaaaaag gaaggcaatt agcgacaaat gtttatgaat tagctaaaga
    48841 actgcctgaa gaagaaaaat tcgttttatc tgaggaaatt acacaaactg ctatcctaat
    48901 gatttcagaa attccaaact ccagcactcc tgcactccag aactaaaaaa ctaaattaac
    48961 aatgagaaaa tacatcgttt ccatcgtggg tcgtccgaat gttggaaaat ccacactttt
    49021 caatcgctta tgccgtaagc gttctgctat agttgatttt gaagcgggaa ttacaagaga
    49081 tcgtaaatat gaagatgtgg aatggaatgg gaaaatattt aaactggtag atacaggcgg
    49141 aattgttttt aacagtatag aaaccataga caaaatggtt ctgcaccagg tgatgttagc
    49201 tattgacgaa agcgatttaa ttatatttat ggtagatgct caaaccggca caacggatat
    49261 tgacaaggaa attgcaaaaa tcctttatcc gcataaagat aaggttatgc tggttgccaa
    49321 taaagctgat aatgaaaaat acgaatggga ggtttatgat ttcctgcagc ttggttttgg
    49381 cgatgttatt cctatttccg ctgctcaagg acgcaatacg ggtgattttt tagatgctgt
    49441 aatagaacgc attccgggaa cacaatccat ttttcaactg gaagaaaagt cctctgctac
    49501 gcgcattgct gttgtcggaa aacccaatgt aggcaaaagt tctattgtaa atctgctttt
    49561 aggcaatcca aaattgattg taactgaaat tcctggcaca accagagatg ctatagatag
    49621 tcctttccgc taccataata aggattatgt tttaattgat acagctggac taagacgaaa
    49681 aaccagagtt aattatggag tggaatattt ttctacctta agaacaattg aagccataga
    49741 cagatgtgat attgtggttt tagtgctcac ggcagatgaa gaacttagcg ttcaagatat
    49801 taaaattgcc agctatgcga aacgcaaaat gaaagaaatt atggtggtct ataataaatg
    49861 ggacttaata gaaaaagaaa ccaatactat caataagtat ctgaaggaat tacattacca
    49921 aatgcccttt ctgcaatttg cccccgtgct ttttatttct gcaaaaacaa ctcagcgcat
    49981 ccataggata atggaaacgg tagctaaaat tgaagaagaa agtgaaaagc ggatttctac
    50041 agcggaactt aaccgtttta tggaaaaagt tattatgcac agacctccta cccatcccag
    50101 tggaaagcat ataaaaatat attacataac acaagcagcg gttaaaccgc ctacttttat
    50161 cttcttttgt aatactcctt ctctgataac ggaaaattat cgccgttatc tgcataatca
    50221 attgcgggaa atgttcaaat ttgaaggagt tagtatcaaa ctcattttta aaggacgcaa
    50281 aaaaggtgag gataaacttg agcttcaata attttcttcc ctatattatt ttactttttg
    50341 cctatctttt aggaagtatt cctattggtt taattgtagg taaggtcttt tatcataagg
    50401 atatccgcaa agaaggcagt ggcaatattg gtgctactaa tgctttaaga gcttttggaa
    50461 cttctacggg acttattgtt ctgctgttag atatggcaaa gggttttatt cctgttatgt
    50521 tagctacatc gttgctggaa aagggcagtc cttttattgc cttaactgcg cttttagtta
    50581 ttttgggtca tatatttccg ctatatttag gttttaaagg cgggaaaggt gtagctacag
    50641 caagtggagc ttttttagca ttaacccctt taagcttgct tatagcattg attaccttta
    50701 taatagttgt agctattttc cgttatgtat ctctggcttc tatttgcgct gctttcgtct
    50761 ttcagtggca ctgttttgct ttattactga aagaggcaaa accggattat gctactgtga
    50821 ttttggtaac gatagtggtt ttgatgatta tcagtaaaca taaaagcaac ctggaacgcc
    50881 tgctgaacaa aacagaaccc aaactgaaat ttacaaaaaa aggaaattag aacaaaaaaa
    50941 tgtgtggaat aattggcgtt ttcggaaacg ataacgcggg acgactcgct gctgtaggac
    51001 tttttgcgga acaacataga gggcaggaaa gttgtggaat ggcagttagt gatggtttgc
    51061 taattcgttt gcacaaaaaa atggggctgg taaaagaagt ttttcacgaa gaacagctta
    51121 atgccttacc cggaaaaata gctattggtc atgtgcgtta tcctacaaaa ggtagcgcta
    51181 cagaatttaa tacccaaccc catttagtgg aaaccctttc aggaccttgt tatgctcttg
    51241 cctctaatgg agatattgta aactatgctc aagtgcgaaa gatgctggaa aatgagaagg
    51301 tctattttaa aagtgacaac gatgccgaat tgcttgttaa atatattgcc taccgtatct
    51361 tacactacaa agaaaatatc ggagatgcta tccgaaattt gatgagggat attaaaggtg
    51421 cctattccag tgtattatgc actcctacag aactttatat gtttcgtgat ccctatagca
    51481 tccgacctat gatttggggt aaaacacctg aaggaactgt aatagttgcc tcagaaagct
    51541 gtgctttgga cactttggga gtgcaagata gaaaagaagt tccccctgct ggaattatta
    51601 aggtaaatga tagtaaaata gaggtgttag ataatgaccc caatctctat cgggaaggca
    51661 attgtgaaaa acactgcatc tttgaacaaa tatatttttc ccgtcccgat agttttcact
    51721 tcggggaaaa tgtttatgct gtgcgcgaaa aaattggggc tgctcttgct cttcaagatg
    51781 aaggtttaaa tcccgatttg gtagttcctg ttcctgattc atcaaatttc atcggtttgg
    51841 gttatgccaa tcaaaacggc atccctcttt ctttgggctt aattcgtaat cactatattg
    51901 gcagaacttt tatcaaaccg gaacaagcag tccgcgatga aagtgtgcgc caaaaattta
    51961 atgtcctccc taatttcttt accggaaaaa gaattgtgct gattgatgac agcattgtgc
    52021 gcggaactac tattcgtaaa atcgtgagat taattaaaga tgccggagca gcagaaattc
    52081 acttaagaat tggtagtccc caaataaaac acagctgcta ttatggaata gatactccca
    52141 ctacagaaga attgatcgcc aatcgtagaa cccttgaaga aattagggaa attaccggtg
    52201 tagatagttt aaaacactta ccattaactg atctgaaaac ctgtgtgcac tgcccggaaa
    52261 attattgtta tgcctgtttt gacgggaatt atcctgtagg agttcctgat gtatacaatt
    52321 gaagcaggaa ggttgatgtc cccgtcaacc acttattaca attcatttcg ttccccaaaa
    52381 gtttacttaa ccgaaaaagg taaggaaacc aaaagaacga cgaggatgtc gttcttccgg
    52441 ccctttataa taggagttat tgatgtattc aattaaacct gaaaaacttg aagaaatcct
    52501 gaaaagtttt caaaatcgcc ggattatagt tcttggcgat attatgctgg atgaatatct
    52561 gtggggaaaa gttcaacgca tttcccctga agcccctgtt cccattatag aagtaaactc
    52621 taccgattac cgtttgggtg gagctgccaa tgtagctatg aatcttagcg tgctgaatgc
    52681 taaagttgat ttggtgggtg tttgcggaaa agacactcaa gccgaaatta tagttcaaca
    52741 gttgaagaaa agagaaatgc ccgttgaagg aattttcttt gatccttctc gtcctacaac
    52801 tttaaaaacc agaattggtt cggtcagtca acaaatagtg cgtttggata gagaagaagt
    52861 tgcagaaata gataacagca tccaaaaaga aatttttaac tatctggtaa aaattatacc
    52921 attgtgcgat gctcttctaa ttgaggacta caataaaggt ctgctgagtt tcaatttaat
    52981 aaccgatgtt ttacaactgg cactaaaaca taaaaaaatg gtagctgtag accccaaata
    53041 ctcaaatttc agctcctatg gcggtgtgga aattttcaag ccaaactacc gtgaactgga
    53101 aagttatatg ggcaggaaac ttaccgatca ggatgacttt gaaaggtcta cggaagaatt
    53161 gcgacaaaga atgagcatca aacatttagt agtaaccaaa ggcagtgaag gaatgtatct
    53221 tttttccgaa aacaaaccag ttaaccatct tcccagtttt gcgcgcgagg tttttgatgt
    53281 ttccggagca ggagataccg ttatcagtgc tttaacttta gcttatcttt atgatcggaa
    53341 cattgaaact gccgctttaa tagccaatca ttctgctgct gtagctgtag ctaaaaaagg
    53401 cactgccagt gttagtattg aagagatttg ggatagtttt aatgctccaa aataaaatca
    53461 ttccctattc tgaaatagca gaattaagcc aaaaactaca tcaacaagga aaaaagattg
    53521 ttttcaccaa tggctgtttt gatattttgc attgcggtca tatcctctat ctggaaaaag
    53581 caaaggcatt gggagatatt cttattttgg gtctgaatag tgatgcttcc gtaaaacgac
    53641 taaagggaaa taaccgtcct attgtttccg aaaaggaaag agccctcgtg gtagcaggtt
    53701 tggaaagtgt ggattatgtt tgtatctttg aacaggacac tccttatgaa ctaatagacc
    53761 ttattcaacc cgatgttttg gttaaaggtg gtgactggac tattgacaaa attgtcggta
    53821 ccgatattgt gcaaaaaaaa ggtggcaaag tcctttccct gaattatgaa cagggtttta
    53881 gcactactaa tataattgaa cgcataaaac aggataaata aaatgcagga agaaataatt
    53941 gaagatagcg ggatcgtaaa aaaggtagaa ggaaatagta ttatagtgga aattgaacgc
    54001 ggaggcggtt gtaacagttg taaaatgcag ggtttttgtt tcaaaaagaa tgaaccggct
    54061 gaatttgaaa tagttacaga tatcccgtta caagtaaatg atagagtgca attggatatt
    54121 tcgcctttag ggagaactct ggtttcacta ttgattttcg gagtgccttt gctgtttttg
    54181 tttttgggct ttttaattgc ctctttgtgg tttactgaaa ttgtttccat tttaatcggt
    54241 tttaccgcta tggcactttc cttcaggata atacggtaca tagatacaaa atatggcaag
    54301 cggttaaaaa taagcattgc gaggaaactt tgaaaataag attgaatgaa atggtctttt
    54361 acggctttca tggagtgaat gcagaagaac gctctttggg acaacgtttt atcgttagcg
    54421 ttgttctgca taccaatccg accatagata aaactattcg ccgtttggaa gatactattg
    54481 attacaccaa aatctatgca gaaattaagg aaattatgga aagccggcaa ttctatttgc
    54541 tggaagattg tgccaatact attgctgata aactattggc tgattttcct ctgctggaaa
    54601 agctgactat ctgcattcaa aaaccctcag ttcctattcc aggaagtttg aaatcggtgg
    54661 aagtgatatt aagccgaacc agaaacaatc aggaggataa cctgtctcaa tgatttatta
    54721 tttatgcctt ggttccaatt tgaataatcc ggtaagtcag cttgatggag caattcagca
    54781 tttggaaaat gacccaggaa taaaaattct ccgcagttct aaaagaatta taaccgagcc
    54841 ctacggaata attaatcaac ctgattttct taaccaggtg ctggaagtgg aaagcagtta
    54901 tcagccccaa gaattgatgg aaagattgct aaatattgaa aaagctatgg gtagaataag
    54961 aaggataaaa tggggaccgc gtaatattga tattgatatt ctattagccg gagacctggt
    55021 tttggatagt agaataacac ctttggaaaa gtcatcccct gaagtaatta ttccccatcc
    55081 cgatttacat aatcgtgaat ttgctttgcg cttgctgaat gaactgattc ctaatgccaa
    55141 acatccttta atgcataaaa cgatttatga attatattat agtatagtag ccgaggagga
    55201 atattatgaa taatctgaca gggattattc ttgctgcagg aaaaggcacg cgtatgaaat
    55261 ccgaaagagc taaagtaact ttaccgcttg ccgataaacc aatgattcaa agagtagtag
    55321 aaacagcttt atccgctaat tgccaaaaaa tatatatagt cgttggctat atgaaaaata
    55381 atgtaatttc tgcagtggaa gataatgaga aaattgaatt tgtggaacag gaagaacaac
    55441 tgggaacagg tcatgcggta atgattacag aacccttatt cacaaacccc gatcaggatg
    55501 tgctgatttt atgtggagat gttcctttgc tttctgcaca aacccttatc cggatttatg
    55561 agaaacataa aagttcttca gctgcttgca cagttctaac tgcctttttg gatgacccag
    55621 gaaaatatgg acgcatctta agagatacat caggcaaaat ttgcggtatc aaagaataca
    55681 aagatgccag cgaagaacaa agaaaaatta aggaatggaa tacaggtatc tattgttttc
    55741 aggtaaagaa tttatttagc gctctcaaac agacctccaa tcacaatcag cagagtgaat
    55801 attatcttac agacactata gaaatccttt atagacaagg caaaaccatt tccaatgtgg
    55861 ttctggagga tttaatggag gtctccggag taaattcaca ggaagaactg gctgccttgg
    55921 aagatattta tgttgatcgt attcggaaaa aatggctcaa taacggagtt gttattcaca
    55981 atccacatag tgtttatatt ggagatgaag taataattga accggatgtg gaaattcacc
    56041 aaaatacggt tattaaaggg aaaagcactt tggaaaaggg ttgtgtgatc ggtccctctt
    56101 gttatttgga acacagcact gtttctcttg attccgtcct gcagggtcat aacattttag
    56161 taaacgctat tctgccggaa catcatatcc tggatttcgg ttcgcatata attgaagaga
    56221 taaggtatga ataacaataa gaaaagcaaa gatgaacatc attatctcta ttctccctgg
    56281 cgattggatt acattttagg ggaaaaagac ggtgattgta taatgtgccg ttttcaaaat
    56341 actgataacg ataaagaaaa cttgatcttg catcgggctg agaattgtta tgtgatgttg
    56401 aatcgctatc cttacaataa cggtcattta atgattattc cttatcaaca ttgtggagct
    56461 ctaaccgaat tgcaagaaga ggcctggctg gaaatggcaa aactcgtcag aaatactgaa
    56521 gccattttga aaaaggtcta taactgtgat ggcataaata tcggtatcaa tcttggttct
    56581 gctgctggag caggaattgc tgaacactta catattcata tagttccacg ctgggttggg
    56641 gatagtaatt ttatgagtgt gataggtggc gaaagagtaa ttccggaatc ctttgattct
    56701 gtctataaca aactggcacc tgaatttgcc aaccttataa aaaattaaga ggttatcggt
    56761 tgaatttgac aaacaacccg cctgaaaata agttgaccac aaaaagaaag atttggttgc
    56821 ctataaccct ggttcttatt gctattgtgg caattgtgct tttttttatc tggaagaaaa
    56881 acactgagga aagcaaacct gtatatagcg aagaacagct tccggcaatt aaagtagtgg
    56941 taactaacgg ttgtggttat gaacgcttgg ctgcagattt tgctgccgct cttaaggata
    57001 aaaatataga agtggttggt ttgtcagaaa cccccaaacc aatatatgat aagacgataa
    57061 tcgttattcg caaaggagat aaggaagatt tggaacggtt gatgaaaatg accggaattc
    57121 aaagatggac aagtgcttac agtgaatatt tcagtgccga ttttgaaatc attgtcggca
    57181 gggattatga gcagtttatt gcttattgaa gggaggatat gttggcggaa tatattagtt
    57241 tggaagtaat tattagctgg ctgaaagaga aaaaagcaga aaatatacgc atttatgatg
    57301 ttcaagaaaa atgtgattat acagacatcg tggttgtttg cgaaggcagt gccgatgtgc
    57361 ataataaagc tatcgccaat tatatggtgg aaatggcaaa aaagaataac ctgcgtattt
    57421 taagtaaaga aggcattgat tatggacatt gggttctgct tgatattgga gatttgattg
    57481 tgcatatttt tttacccgaa acaagggact attataatat agatgagctt ttggcaaaaa
    57541 tagccaaaag acctgttcaa gagaatgaga gatgattaaa gaacttttac ataagaacat
    57601 agttgaggca ttggataatc tgaaactcaa tcacgaaaag gaatttaccg tagaaattcc
    57661 caataatcca gagtttggag attattctac caatgccgct ttggttttag gtaaagaaaa
    57721 taagatgaaa ccaaaagatt tggcagaaaa aatagtaaaa gaactgaaaa aaaacaaagc
    57781 atataaaaaa ctggaaattg ccggaccggg tttcataaac ttctttcttt caccttccct
    57841 gtatcagcag gttttttggg aaatttacaa aagtgaggac tatggtaaaa gtgatttcgg
    57901 caaccaggaa aaaatcctgc tggaatttgt aagtgccaat cctacaggtc ctttaaatat
    57961 agttagtgcc agggcagccg cttttggaga tacactatat agaataatga aatatgtggg
    58021 ctacgctccg gcaagggaat tttatattaa cgatgccgga aatcaggttg atattttagc
    58081 tgaatcgttg gaattgcgtt tacgggaaat ccatggcgag aaaataggtg aattccctta
    58141 tgaggcatat catggagaat atgttaaaca tctggctcac cgtttgaatg ctttggaagg
    58201 gacaagggtt tttatgttgc cgcaaaaaga ccgtttagaa cacttgaagg aatttgctct
    58261 ttccgaactg ctggaaatgc aaagaaattc ccttgaaaaa tttggtgttt tttttgaggg
    58321 ttgggtttcc gaaaaagctc tacgcgcaga aggtgtagta gaggaagttc tttcctattt
    58381 aaccgaagcg gattgcactt atgaaaaaga agatgctatc tggtttaaca gcacaaaata
    58441 tggtgacgat aaagatagag tgctgatgaa atcggatggt tcaattacct attttgtgcc
    58501 tgatgttgcc tatcatttaa caaaaatcca gcgtggtttc accaagttaa ttgatatctt
    58561 cggtcctgat catcatggtt atgttccgcg tatcagagct gcctttcgtg ctttgaacta
    58621 tgatgattcc atattggaat ttattttcct ccagcaggtt aatatctttg aaagtggcga
    58681 aagggttaaa atgagtaaac gagccggtaa aatcgttact atggatgacc ttattagtgt
    58741 ggtaggcaaa gatgccgcgc gttatttttt cattgcccgc aaagctaatg cccacttaaa
    58801 ttttgatttg gaactggcac tgaagaaaaa caacgaaaat cctgtttatt actgtcaata
    58861 tgcgcatgcc cgcatttgca gtattatcaa aaaagcgcgc agtgagaaaa tttaccccaa
    58921 gcacttcaag aaagaaatga taaaaaagct gaataaacca gaggaactgg cactgattca
    58981 aaaaatggct gatttgcctg aattgcttat tttgatagca gtccaccggg aacctcatcg
    59041 cttaactact tacattgaag agctggcgtc tttactgcac cattattatg aaaagtatca
    59101 aatcgtaaat gttaaaaatc ctgctctttc tcaagcacga ttgatgctgc tatatacggt
    59161 tagaaaagtt atgcaaatct gctttgagtt aatgggcatc agtgctcctg agacgatgta
    59221 aaaaataaag tgaagataat ggataatgct tatagcattc gtttatcctg tgaggatgat
    59281 tttgatgcca tagaggaaat agaacatcag gtatattcca atccctggcc tcccgaagct
    59341 ttttcccgta tgctttttcc ttgggctttt actttgcttt atggcaagga agttataggt
    59401 tacatctttt attgcggtgt aaaagaagaa atggttatcg taaattttgc cgttaaacct
    59461 gattttcagg gtaagggctt aggagaatat ctgctcacgg aaacaatgcg gatgatgaat
    59521 aataatggcg ttcaacgctt ttatctggat gtgagaatat ccaactataa agcgcgtcgt
    59581 ctttacgaaa aggtaggatt tcaagagatt ggaatccgca agcaatatta caacaatccc
    59641 gatgaggatg ctttagttat gggaatggaa ttcaatgaca tacgagtatg actatttagt
    59701 tatcggtagt ggtattgccg gtttaattta tgctctgcag gtttccaaat atggaaaagt
    59761 agcagtggta accaaaagtt cactaaatga ttgtaataca gaccacgccc aaggcggaat
    59821 tgctgctgcc atagattctc tggattcctt tgaggcacat attgaggata cttatcaggc
    59881 aggagcagga ttgggtaagc gtcaggttat tactgaagta atttcttcag gacccaaact
    59941 gattcagtat cttatagatt tgggtacgga ttttacgcgt cgtgatccca attacgatat
    60001 ctgcttggaa aatctttccc tcacaatgga agggggtcac acacgcagaa gaattgctta
    60061 tgcagctgat tctacaggcc atcagattat ggaaacatta attgctcaat gccagaaaaa
    60121 tcccaatatt gaaatctttg aatattatat cgctatagac cttataacac agcaccatgt
    60181 agttcaggac gaaggtttta ttccgggaat ttcctgctgg ggtgcttatg ttttaaatat
    60241 taatgaaaac cgggtggata tttttaaagc ccgaaaaaca atgatggcta caggtggtgc
    60301 agctcaaatc tattctccgg atacaaatcc caaagtggca acaggtgatg gaatggctat
    60361 ggctcgtttg gcaggagctc gtttagctaa tatggaattt gttcagtttc atcccactgc
    60421 gttttggagt ccggatggtg atactttttt aataagtgaa tcgttaagag gagaaggagc
    60481 tgttttacgc ctttccgatg gtagcacttt tatggaaaat taccatccgc agggaaattt
    60541 agctcccagg gatattgttt cccgggcaat agacagcgaa ctgaaaaaac gaggggaaaa
    60601 attctgttat ctggatgcca ctattgtgcc tgcagaacag ttattgcgac attttccctc
    60661 catcaataat atgctgaaag cccgaggaat tgacttcacc aaagaaccaa tacctgtagc
    60721 tccggcagct cattatttct gtggaggagt gctttctacc atagaaggca tcaccgatat
    60781 ccgtaattta tttgttgccg gagaagttgc ctgcagtggt ttacatggag ctaaccgttt
    60841 ggcatccaat tcgttactgg aagcgctggt gatggcttat aaagcgggaa atcatccttc
    60901 caacctggaa gaagttcagt ttccctgtat tccggaatgg aaagtaattg atgaattcaa
    60961 cgaaaacgaa tgggtggtta ttagtcacaa ccgtgaaatt attggaacta ttatgcaggg
    61021 ttatgccggt attagaagaa gccgacggtt actaaaatat gctctttccc gtctggaaaa
    61081 tatctataac gaaattaata acttctatca gcataatgca gtccgcaaag aagtagtgga
    61141 aacgcgaaat atggctataa ttgctatcgc tgttgttaaa agtgctttaa tgcgtaaaga
    61201 aagccgcgga gctcattttt tggtggataa tcctgaacgc gatgatgaac actataaaca
    61261 cgacacaatt atttagtgag agtactgtgc acaataagag ttctcactgc gtcagttctc
    61321 acttggaaaa tctaacaccc aaggactcat tttcaggcaa aggtcttttt attacctttg
    61381 aaggaattga aggcagcgga aaaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61501 nnnnntttca ggcaaaggtc tttttattac ctttgaagga attgaaggca gcggaaaaac
    61561 aacgcaaatc ataatgctgg tggaagtgtt aaaagctaag ggcattcctt atgtaactac
    61621 ccgcgaacct ggtggcacaa aaatatctga agccatccgt gatttactgc taaaccctga
    61681 gtttatggaa atgaaaccag aaacagaatt gctgctttac tgtgcaagta gagctcaaca
    61741 tactgcagaa ttgattcttc ctgcgctgga agaaggaaaa atagtgatca gtgatcgcta
    61801 ttttgattcc acctatgcct atcagggagc tgccagagag cttgatgaag aagtaataaa
    61861 tgttttaacc aggttttcca cttatggcag aaatcctgat ctgactttcc ttttagacct
    61921 tccggtggaa gttggacttg ccagaattaa aaaccgcaat ttagaccgct tggaacagga
    61981 aaccctcagt tttcacgaaa aagtgcggcg tcaattttta tctattgcca ataaataccc
    62041 ttccagatat attgttcttg atggagaaag ccaaccggaa attattcacc gtcagattct
    62101 ctccactctg caacccttta taggagaata aaatgaatag acctaagcag aaacttgctc
    62161 tgatctcaat aatcagtatc tgggtactaa gtgctttact acttattgta gggacgaatg
    62221 cttatgccca aaaccgtccc caaagtacta atctatattc ccaagtgcag cttttcagtg
    62281 aagttcttta taagctaaaa caatactatg taaccgattt aaatgatgaa gaactgatta
    62341 aagctgccat agacggtatg cttagttcca ctgatcctca tactacctat tttactcctg
    62401 aagaatttaa ggaatttact acaactacta aaggttcctt tggcggttta ggaattcaaa
    62461 tagataaaat agggaactat ataaccgttg tctctccaat tgaaggaact cctgcttata
    62521 gaatgggaat tactgccggc gatagaatta tcaaggtaga tgatgtatcc atagttggtt
    62581 tttctaccga tgaagctgta aaaagaatgc gaggtgatgt aggcaccaaa gttaaaatta
    62641 ccatcagtcg tcccggttta cctgaaccta ttgattttga attaacccgt gagaccatta
    62701 aaataaagag cattccttac agtttcaaac ttgataatgg tgtcggctac attcgcatca
    62761 ctcaatttag tgaaaatact ttaaccgagc taagggctgc tcttgataac ctggaaaaac
    62821 aaaatgttaa gggcttgatt atagacctgc gttggaatcc gggaggactt ttagaacagg
    62881 cagtggatac ggttaatgaa tttatcggga aaaaccgttt agtagtggaa accaaaggta
    62941 gaacggaaaa taagcaactc tataccagat ttgatgttaa agaaagaaat tatcctatta
    63001 tcgtgcttgt gaatgaagca tctgcatccg catcggaaat ttttgctggc agtttacagg
    63061 attgggataa gggcttagtt atgggcaaaa ataccttcgg caaaggtagt gtgcagcagc
    63121 ttattcctct ttccaacggc aacggaatta aaattactac tgcctattat tacatcaaaa
    63181 gcggacgctg cattcataaa atgagcaatg ataacatcct gaaaggtaaa gaagtaagcg
    63241 aagaagacct gaaaaaagaa agtgaaacca acctccaaca ggtctattat actgccaata
    63301 accgtaaggt ttatggagga ggaggaattc aacctgatat taccgtagaa agcgattttc
    63361 tcaccctttt cggagttgaa ttacgccgca aaaatgtttt cttcaatttc gccgtggact
    63421 atttagtcca acataaccac cagtttgata tcaataccga aattgataac aaaattatga
    63481 acgaattcct gacttatgtt tcaaaaaatg atattaaata cactcaggca gatgtagata
    63541 gtgccaatag ttttatccgc acttctatta aaagtgaact cgtccgcaaa gtgcatggtg
    63601 acgaagaagc atataaaata actatttctc aggataaaca gttgatggaa gcaatttctc
    63661 tgtttgatcg tttcagaacc ttagaacaaa tgtttacata cgccgcttcc ctaaacgaga
    63721 aaaaagaaaa atagggagtg tgttcaacca atagggtagt tcaaataaca caatacctta
    63781 agaaaaaaaa tattattgtt cattacctga ggtaattgaa ccaacaaagg ttgccgtcgc
    63841 acccgcctag tcaaagcgac ggtttttttt cctttagaac tttgaagaat tttttttcgt
    63901 atcctggtta gctacaaatg ctgttattaa gcggactatt ttctttttct tttaaaccta
    63961 aattatcttc agtttttatg gtttgggcag ataaaattta tcaaaccttt ttctcagata
    64021 tatgtttata gcggtataat cggtaatcgt aactccactg aagataccaa tcataatcat
    64081 catttggtat ttaatagcta ccagaggaga agcaccacct aaaatttgac cggtcatcat
    64141 tcctggcaaa gttacaagtc ccatagaggc aattgtaagc aattccggca ttagagctgc
    64201 ctgcattgct tttctgaaag caggaaggat agcttcccat tggcttgcac ccaaagaaag
    64261 gcgggtgtaa tatgctttcc agttttcgct aagaccactt tcaaagcgtt ccagggcaag
    64321 ggaacaatta ttcatactgt ttcctaaaat cataccatag atgggaatta gaaaccttgg
    64381 agcaaacaag ggttctggac gcagaacaac aattatcagc caaggcataa caatcagcgt
    64441 agtagttaaa agtgaaaata tgagaaccgg taaaagaatt tttttttgaa atttgagccg
    64501 ccctttcaaa gttaaaacag catttgccaa cataataagc atccaaagaa aggcaagcca
    64561 cggattttct ctgttgaata taaactgtag aattaagcca ataattgcca gttgcacaat
    64621 cattctggca aaagaattaa aaagttgacc gctcaatttc agtttcaaat tactaaaaat
    64681 caaaagcgga aaaattaaaa gtaaagctgc cagcatcaat ctataaatac tgatatccat
    64741 tattttatat cctcctgtct atgattgttc catattccag aacccaattt ctttgccaaa
    64801 ggttttgcca aagcggatcg tgggaaatgg ctattactgt tcccgcaaaa ttgttaaaaa
    64861 tacattcgga aataatcccg gaggtctttt gatccagagc ggaagtaatt tcatcaagta
    64921 ataaaatatc cggctgcaat tgcagaactc tgattaaggc aatgcgttgt ttttcaccaa
    64981 ccgaaagctg agaagtaggt ttttgcagat attcataaga caattgaaat gctgtgaata
    65041 aatttttaat tctttcctgc ctatcgggta accgcctgtg ctttaaagaa ctgtaatgaa
    65101 ggggaaggtc taaaacctct tgcacattag catcttctaa atagggttct tgtaacacca
    65161 tacaaatctt actgcgtaat atatcgggct gatattctac aatatttttc ccttcaaaca
    65221 gaatttcgcc ttcataattt ctgttcatca ggttcaaagt agaaagcaga gaactttttc
    65281 cgcttccggt aattccggta attaaaattc gttctccctt ttccactttt agttgtatat
    65341 catttagtaa aacttgatca ccataaaaaa ggcgttttat atttattgac agtatttccg
    65401 acattagcaa tccttctttg ctttttttat atcttttacc acaactgcag attcagtcaa
    65461 gaaaattgtc tatctaatag taaataagta aggaatacct tatgtatatt ttttctactt
    65521 attgcttatg aacagttatc atagacaaaa tatcgtttca ggatagcgat tattggactt
    65581 tcctattagc taaattaaaa gaacaccacc cgtttttaac tattaattta acaccacaat
    65641 catttcttaa ttttttcatt tctctttgaa tattggattg aatagctgga actactttag
    65701 taggaataac aaatgcatat ctttcggagc ttaaattaat ccttcaaaaa tcataaaaaa
    65761 gtgataaaat ccctaagcgt attttttaag atggtttgtc aaattggaaa ttgtataatt
    65821 ttgtagtttg tattacatct aatatcaaat taaaattatt cctgactaag gcaacctgaa
    65881 attataatat tttacttgac aacgcgatac tggattatat aaaggtttat atgtagtgat
    65941 aaaacactga agagaaatta tattattcaa aaaaagaaag tagaggtaag aatggttaat
    66001 tataaggaaa tcaaggttga atttgattac cctataaata attatactat gagcatcttg
    66061 aatgccttag aaaagaaata tggtgaaagt aatattcatt ttgattataa tgataatcaa
    66121 gttttagttt atcttaataa tattgatttt gaaatgttca aatcagatat agaagaatta
    66181 tcacttaata ataataacaa tgatttaaat aaaatatcaa atgcgataag taacatcaag
    66241 agttatggta taaagggaaa aaaacgtctt tatgtaggat atggagatga aagaaaaact
    66301 gttaatagaa aagaaaagga aaaaaaacga gaaatcttct attatacccg gggaaacgac
    66361 ttttctgaga taaataactt attgcctgat aaatatatgg atcagatcgt atgtggcgat
    66421 agtaaaataa tactatctca attacccaat aattgtattg atttaatttt tacttctccc
    66481 ccttataatt ttggtttgga ttatgaagaa aatgaggatg atcagcattg ggaaaaatac
    66541 tttgaaaccc tctttgctat ttttgatgaa tgcatacgag ttcttaagta tggtggaaga
    66601 ttcattataa atgttcaacc cctattttca gattatattc ctactcatca cataataagt
    66661 aactatttaa tgcaaaaaaa gctaatctgg aagggagaaa tattatggga gaaaaataat
    66721 tataattgta aatatacagc ttggggatca tggaaaagcc caagtaatcc ctatctgaaa
    66781 tatacttggg aatttataga aatattttgt aaaggcactc ttaaaaaaat cggagacgat
    66841 aaaaagattg atattagtgg agatgagttt aaaaaatggg tctatgcaaa atggtctatc
    66901 gctcctgaaa gaagaatgaa agaatttaat catcctgcta tgtttcccga agaacttgcc
    66961 accaaagtta ttaaactttt tagctatcaa gaagatattg tgttggaccc gtttaacgga
    67021 gtaggaacta caacattagt ggcaaaattg aataaaaggc attatttggg gattgatatt
    67081 tccagtgaat attgttctaa agcagaagaa agattatcat cctttttatt ttaattatta
    67141 aactattttt attagatgat atgaaatata atatagaatt acctttttat gtgttacatt
    67201 ttaaggttgt ttaattaatt tggtgggata ttatgaaaat tccgactgtt gaagacctaa
    67261 ttaatatcta tgatgctatt attaaaggca ttgataagga agcattaaat agtaaagata
    67321 gagcttatgg tggaataatt agagcaggaa agggtagttt agtagaaaat attgctaaag
    67381 gtataattca gatagcatgg gatcgcttaa atggagatca aacacattta tctttaaata
    67441 agaaaaaagt tcgtatacca ataaaagttg attatgtaaa aagaataaaa aatcctgaaa
    67501 taaaatcata tattcaagat cattttggta attattatta tgaactgaat atagatgttc
    67561 cagtttacat tgataatgaa ttagttttgg gcgttgaatg taaatcatat accgaaaatg
    67621 caatgttaaa aagaatattg gtggatttta ccttattaaa aacccaatat ccaaccttag
    67681 attgtgtttt actccaatta gaaagccagt taaatgggga ttattctaat atcagtcagt
    67741 caattatctt tggtagtagt tccactcata ctctattatc ctattttgat gttgatttat
    67801 atataatcac cttacttcag ggtgaaagga aagtaaaaaa acctattcat aaaaaagaat
    67861 actttaagga acttacttct gaatctatag aaaaagctat ttctaccttc caaaatattc
    67921 ttagtaagta taaaaagtag ttggataaat cataatgcaa ttttcatatt gaaaatgaag
    67981 ttaatatttt taattttatt tggattcatt gtctcacaag aatgataatt agcttttcag
    68041 gcaaaaaata acataataag aaattggggt aaaaatagtg ggcaaatcct gattacgcag
    68101 aaatgaatag aggatttcca ttcaaaaaat gatatatgaa aaaagggaat gctccaatta
    68161 ttttttgttt acataacccg gtaatgacga tgtaacaatc aagtaacaac tccgtaacga
    68221 gattacggaa gtgttacgga agtgttacgg tagtgttacg gagtcgttta ataattaact
    68281 atgtaacaat caattataca atttaagctg aaacggacaa ataatggggt taacatacac
    68341 atattttagt tagttacatc aatcttggca gtaatggttc tcttgttttt taggttcagg
    68401 ttagaactaa taaaattatt gttataattg ctttattaat atgaaagttt taggaaaaac
    68461 aggttacggt tttttcccta cgctggcaat atagataaca aattcttcaa tgccgtccaa
    68521 accaagtgcc tcattcattg cttcatccaa aaaagcgcca atcatacagg caccagcatc
    68581 aatggcttct gctgcaagat ggatattttg tccgatatgt cctgcgtcca gatagagata
    68641 acgatatcct cgttgtccat atctccagac ggttctatag ggaacagccg ttaaaatgaa
    68701 gttcactgcc gctttgggaa tcatttcctg aaacatacat ccctcgtgaa ttttagcggc
    68761 aatttcaggg gaatcatcgt ataaaaccag acaatgtttt acaggatgat agtaatataa
    68821 gccgggtttg ataccttcca ctctgcggat gtctaaaaat gtctccaaag gatgtcgcga
    68881 gccggcagaa ggaacattgc gaaaagtgat ttccatttgt tctgaagaac gaaaatctct
    68941 tgcccaggaa gaagcccaca gcaaaaaaga gagttcttcc tgttgcagag gaatagcgct
    69001 atatttacgc acacttctac gctgttcaat ggctttggct aaaggcatat ctggcatttt
    69061 taaggttcct attgccggta aagggacaat ttctccgctg cgaactttta tggcatccgg
    69121 acggggaact cctaatgttt ggtcactttc gcgttcataa atgtaacggg ttaggcgggt
    69181 aaaatcctgt cctatatttt cggcaacagg tcttaacatt tcaatttctt tgaggacatc
    69241 ctgcagattt ttctcaaaaa attcaaaatc ctcttcactg attcctgctt gtttaataat
    69301 atcttccggg gaatagcccc gggttttgta atacaaatag ccaaaggttt tcatatccat
    69361 ttttggtttc tccttttctt acagcaggtc ttgccgattt tgttgttcca aatagtccac
    69421 aattttcttt acttcttccg ccatctcttt gttacaaagc aaaatagcat ccttggttgt
    69481 aatcaaacaa aggtcgttta cgccaattaa agcagtgaat ttatcggaaa ctacatagtt
    69541 atttttagcg gaaattgcct ttcctgatgc ggagaaatag ttattatccg cgtctcctgc
    69601 tgttaaatcg gcaagcgatt tccagcttcc aacatcgctc caatcgtagg aaacaggaat
    69661 aactccccgg aaatttgcct tttccattat accaatatca ataggaatac gtggcatttg
    69721 ggcataaata gaggtaatat ctttatccaa accttcattt ttccatatct taccaatttc
    69781 gttagttaag ccataaacat cgggcaatag ggtttcaaat gccgtagaaa tactttccag
    69841 tgaccagcaa aacataccgc tgttccaaaa ataagttcct tttgccaaaa attcttctgc
    69901 ggtctgctta tcgggctttt ccttaaaatg gagaactttc ctgaccccgg atgcaatttc
    69961 ttctcccgct tcaatatagc catatcccgt ggcaggataa accggaacaa taccaaaagt
    70021 aatcaaacac cctttttctg cttctctttc cgcaggattt aacgattcca aaaacttttc
    70081 ctgatcccga ataagatggt ctgcaggcaa aacgagcatt gtttctttct gcccgtaaat
    70141 atttttcaga tattctatac taagagctat gcagggtgct gtgttcattc caaaaggttc
    70201 aataatgata ttttgctggg gaagatttgg tagctgttgt ttaattaact ctacctgact
    70261 actcgcggtt acaatataaa tatcggcaag aggaaaacgg ggtaaaagac gctcaaccgt
    70321 catttgtaaa agagaaaggt cgccaaaaag gcgtaagaac tgtttcggaa gattttttct
    70381 gctgaggggc caaaaacggg ttcccactcc tcctgccata ataagagcaa tcattgttca
    70441 actacctgca catctttggg ataggtatat tgccaaacgg aggcaggaat tttggcattg
    70501 gttttgatgc tggaaaaatt ataggtaact ttatcgttat tacgattagt ccaggttaaa
    70561 gattgcacta ttccgctttc cgaatttact acagcactta tagatacgat aaccgggtcg
    70621 tcaaaaggtt ttaagaaaac attcacctgg tttcctttac cctgctgaat gttgacagtg
    70681 gactttttcc aatatagttg taaaatttcc accggattca tttttccaaa ttcaggacgc
    70741 atcttgctgc ggaatacggt attgctttga gaatcataaa gttcaacaat tccaccagaa
    70801 acacttagcc gttgaaaata aggtttatta tagcgaataa ccattcttcc tttggtaaaa
    70861 tagatatttc cgtggtaggt gatgctttta tcaagctgag caaaatagtt atcctgtttt
    70921 ataactgcct gaaaactgct taaacttccg taggtcttgg caattttttg atataatgct
    70981 tcactgctga tgccaaaaag aaaattacag cacagtaaaa ggataagggt aattatttta
    71041 gcggtcttca tctatcactc ctatgcgaat taagtcctct cgggtagcta aaacatcgcg
    71101 ggacttactg cccaaatgag gacctataat tctggctctt tctaaaaggt caatcaagcg
    71161 tcccgctctg gcatagccaa ttttaaaatg cctttgcaac atagaaacgg aagcagttcc
    71221 agtggaaaca accacctttg ctgcttcggg aaataattca tcgtcgtaat caaaaaagcc
    71281 gacaccttca ttcttttcaa taacaagcga aaaatcctgt ttgggtttgg gctgtgtagc
    71341 caaaaagtta cacaccctgg caatttccgc gtcggaaaca aaagcaccat gaatgcgttc
    71401 cggtaatgct tttcccgggg gtaaaaacag catatctcca tttcctaaaa gtcgctcagc
    71461 tccaatcata tctaaaataa cgcgggaatc tactcgggaa gatacctgaa aagcaatccg
    71521 ggcaggaaaa ttggctttga taattcctgt tataacttta atggatggtc gctgagttgc
    71581 taaaattaaa tgcattccca cagctcttga catttgggct aaacgcgtta taggcatttc
    71641 aatatccttg ccgctggtca taattaaatc ggcaaattca tccactatga taactatata
    71701 aggcaggggt tcaaggtctt catcttctcg gcatttttca ttgtagccaa ttatatcacg
    71761 cactttagct tcctgcaaaa cttcataacg cctttccatt tcgcgcactg cccaaataaa
    71821 ggtctccaaa gcagtgtccg catcggttac aacctgcccg attaaatgag gaagttcatt
    71881 gtaacctgcc agttcaaccc gcttgggatc aattaaaata aggcgcagtt catcaggttt
    71941 ggtgcgcata atcaaactca ttattatagt attgatacaa acgcttttcc cgcttcccgt
    72001 agctcctgca attaaaagat ggggcatttt tgccaaatcg gctacaatcg gttttccggc
    72061 aatatcttta cctaaaccga aagctaattt tgatgttgtt tgccgcatct gttccgaaag
    72121 caagaggtct ttcaaataaa tcatatctct tgccagattg ggaatttcaa ttcctattaa
    72181 acctcgtccc ggaataggtg cctgcactct gattgatttt gctttaatag ccaaagccaa
    72241 atcgtctgcc aaagaagtaa aacggctaac tttgatgcct ttagcgggtt ccagttcata
    72301 ttgggtaatt ataggaccga tatttacatt cttaacttcc gcttcaatac caaattctgc
    72361 cagtttgctc tttaatactt gactggtagt gataatttga ttttcaattt ctttgcgttc
    72421 gcgttcggaa agtttaattg ggctttccaa aaagtcatct atggaaggta actgatattc
    72481 ccggtctgca tcttcttccg tagcaaccgg cttgggttta tactctttag ggatttttat
    72541 aatatcttca aaaaaaggtt ccggaggagg attcggctga ttgtgatttt gaataacggg
    72601 aatcgtttcc tctgctgctt ttggaacggg attaagatta ttggcagtaa tagttggctg
    72661 gacagttttc gttctttgtt cctttttgcg ttgcaggttt tccctggcaa ctaaaatcca
    72721 gctttttagt cgttcatatt caatcagcat aattgccgtt aaaatcatag ctccaatcag
    72781 gataattttg gctccgatgc cggaaaaaac agcagctaaa attcggtaaa caaataaagg
    72841 cacaacaccg ggtatatctt gcttggatac aatcaatgcc tgcaggaaaa acataattat
    72901 caataacaga taacctttct gcctatgttt taacgattcc ggagttaaaa aataatgcaa
    72961 cgctacaata gctataataa taaaaccgat taaagaaaac cagtaaccga aaagataaat
    73021 aaaaatataa ccgcaaacaa tgccaaaaat acctaccgga ttattagttg tggaattgcc
    73081 ttccttaaac caggaaatta tattaccgct tgcttttagg ttatccagat catctttaat
    73141 ggaaatataa cccactgtaa gagataaaat aattaaacag gacagcaaac agataatacc
    73201 gctaagaatc cttttagtta acggatttcc ttttttattt gtctttcggt tatttacttt
    73261 tcgttggggt ttatttgcca ctgttatctg ctcccagatt ttttatacat taaaacggat
    73321 ggaaagaata tctccatcct taacaatata atcctttcct tccaagcgga aaagtccttt
    73381 ttctttaagc gccttttcac tgccataagt tataagatca tcatactgaa aacactctgc
    73441 acgaataaaa ccacgcgcca ggtcactatg aattttccct gcagcagtta cggcattagc
    73501 acctttttct attgtccagg cacgaacttc atcaggacca acagtgaaaa aactgatata
    73561 acccatcagc tgataagtcc aatgtgttaa gcgatcacga atggattcac taatccccat
    73621 atcttccata aacatttgtg cttcttcatt ttccagtttg ctaagctctt cttcaaactt
    73681 tccggcaatg gtataggcat aataaccctt ttcggtaaat gtttttaagg tctcatccca
    73741 ggcatttata ttatcctcgt tgcagttaat caaaatcaac attggcttgg cagaaaagaa
    73801 ttgaaaaccg cgtattgcct tctcttcttc cgggttaagt tccaaattta ttaaggggtt
    73861 attattctgg agaaagtccc tgattttgag taatgccttt tcttcaattt gcacagctgg
    73921 ggttttaact cctcttttat aacccagttc aattttttcc agccgctttt ctgccataat
    73981 caaatcatat agtaacatct cttcctgaaa gtgttgaaat tgttctatcg gggtgcctaa
    74041 aggaaaaaga ccctccagtt cttcatccct aaagccacgc agaactaaga taaatgcttc
    74101 tgcattttta atctctgcaa gcaaagcgtt ttcagatatc tcgctctctt tataaaaaat
    74161 accgggataa tcgtgaaatt caattgtggc atatattgtc tttttcggtt tgtaaagctc
    74221 cgaaagctta tcaatacgct catctgcaac ttgcacaaca ccaatattgg cttccaaaga
    74281 gggtggagca tatttatccg tgctctgttc acctctggtt aatgcattga aaatagttgt
    74341 tttgcctgtt ttaggcagac cgataattcc tgctttcatc tatgtttttt ccttctttaa
    74401 agataacagt gctttcactt caccgggctc taatagacgc catctgccaa caggtaggtc
    74461 tttcagtttt aagggaccga actgtaagcg ttttaaatat gtaacttttg ctccaactgc
    74521 ttctatcatt tggcggattt gtctttttct gccttcggta ataactattt ttaaggtcat
    74581 cccgttggcg tttttggact taacaaaaat acctgcaggc agagtttttc ccccttcaat
    74641 ttccacacct ttacgcaaca tattcagttc catattattc aggggacggt tgatatctac
    74701 gcgataaacc ttttccactt tgtttttggg atgagttaat agatttatta aagccgtgtc
    74761 attagtaaaa aacaacagcc cttcactttc tttatccagg cgtccagcat aagcaagttg
    74821 ttttcctttt ggcggtaaaa gagaataaac cgtttttctt cccagctcat cattttggga
    74881 aacaacataa ccacgcggtt tattaagaat taggtaaaac ttttctttgg cgggttgaat
    74941 cagttttcca tctaaagtaa ctttatcttt gccgggaata atctgtcggg aaaggtcttg
    75001 cactaaatta ccgtttactg caattctacc agtagttatg aattcttcta cttttcttct
    75061 gctgcctaaa ccgcaatcag ctaaataacg gttcagacga agggttttac cagccctcaa
    75121 tactgttttg gaggatggtt ttatagagtt tggagatgag ctcttcaact gcttcctctt
    75181 tagttttaaa tttggcagtg cctccttcgg caactgcata aagttccatt acagtcaaac
    75241 cgctgttttg gtaaagcact gtattattga ttaaatcggt aaaggtaaca gaaacgacca
    75301 tttttacctg ataatcctga acttgattag ctgcatcgta actgtaaaca ttttcggaaa
    75361 aacttaaaat ggctccttcc agagtgcaat cggggtcttc tgtcaccagt ttcaaccttc
    75421 cgtcttccct gatttcccgg ttcaaataat taagcagttt atcccccaac ccgtattcgc
    75481 tggttctatt ttcaaaagcc agaattctaa ttttccgcaa atgcgggtaa gcattggaat
    75541 aaaccgaata atgacaactg cttaagacaa ccaaaagcag aatcgtataa ataaatttct
    75601 tgaccattat tccatcttca aaaaatgctt atattattgt caagctaaac cttgccaaag
    75661 cagatataaa ataatgataa aacctaaaaa aggagttctt ttatggcgaa aaaatttatc
    75721 tctaaagctg aagctgccaa gaagttaaag gtttcggagc gcgtcattca ggaaatgatt
    75781 gctacgaatg tttttgaaag taagctcgta ggaaaaaatg ttaaaataga cgaagattcc
    75841 ttaaacgagt ggctggaaaa tttgaatgag acagaagaaa aaatgctcgc tttaaagcgg
    75901 gttatctgtc attttgaaga atatatgcgt cccgaaaaca ttttcctgga ttttacggca
    75961 gaaaacaaat atgatgctat ccgaatctta agtgaaaagg cgaaagagct gaaacttgtg
    76021 cgcgatgcac gctggcttta tgaagttgta gttgctcgtg aagaattggt ttctacagcc
    76081 attggtaatg gagttgcctt gcttcatccg cgtcatttgc atccttctaa aattaaggct
    76141 cccagcattt tgtttggtcg ttcttccgaa cctgtagatt ttgatgctcc tgataacaaa
    76201 ccggtgaata ttttctttat gcttcttttg cataatgata aacagcacct tttctcgctt
    76261 tcttacattt cccgcttagt tatgaatcag gatatcttaa agggtttaac ggaagcaaag
    76321 gatgccaaag aaattcacca acttcttact gtaattcccg aacaaactta aacaatgcat
    76381 ccccaagtca ggattattat aggcagtaaa agcgatttgg agaaaattac tcctgccagg
    76441 gaaatactgg aggagtttga ggtctcttac gacctgcata tttcttctgc tcaccgcaat
    76501 cctgaaaaaa caattgccct tgctcaaaat gccaaagcgg aaggaataga aataattatt
    76561 gccggagccg gtttatctgc tcatttacca ggatttattg ccgctcatac aactttgcct
    76621 gtaattggca tacctattgc ctcaggtgcc ttaaatggat tggatgctct cttttccatt
    76681 gtccaaatgc cttcaggagt tcctgttgcc tgtatggcaa taaacggagc taaaaatgca
    76741 gctctgtatg ctatccagat attagctttg aaagacaaag acctggaaaa gaaattttct
    76801 gcctataaaa aaggactgga gggctaaaga ttcaggtttt tgccttaccc tgattggcaa
    76861 ctgcctgttg agcagattcc agttctgctt tatctgccag ataataattt tttaccggtt
    76921 gtaaattatc atcaaattcg tagattagtg gtatgccggt aggaatatta agaccaacaa
    76981 tatcagcatc gctgatttta cttaaacttt ttactatagc gcgtaaacta ttaccgtgtg
    77041 cagaaactat catttttcta cctgcagcta aacgaggcat aattatttca ttccagaaag
    77101 gcattgttct tttaacggta tctttcagcg attcacaaag aggaagttga tcttccctta
    77161 aatttgcata tcggggatca aatcccggat aacgaggatc acttttttcc aaaggtggtg
    77221 gaggtgtatc ataacttcta cgccaaagta aaacttgttc ttctccatat ttagcagccg
    77281 tttcagcttt gttcaaacct tgcaaagcac catagtgacg ttcattaagt cgccaatcat
    77341 gatgaacagg aatatacatc aaatccattt catccaaaac gatccacaaa gtcctgatag
    77401 ctcttttcaa tacggaagtc caggtttctt caaaaacaaa accttcttcc ttcagtcgtt
    77461 tacctgcatt atgtgcctca actataccct tttcggaaag gtctacatct gtccagcccg
    77521 tgaacagatt ttctttgttc caagtgcttt caccgtgacg cagtaatact attttataca
    77581 taactaactc ctttttaagt gttgttttcc tcttttcttg ccaggacaaa aaagaaatcc
    77641 gagagtcggt ttacataact aagcagttct ggagcaatat tttccttacg ggaaagggct
    77701 attatttttc tttcggctct tcgggcaacg gttctgcaga tatcaagctt ggcagaaaca
    77761 ggatttccgc cgggaataat aaaacccttt aagtgcaggt ttttttccag agaataaaca
    77821 agctccgtaa tattatccac atccttttgc gtaatcgctt ttgcaaaagt tcctttttta
    77881 gttgccaatt gtcccataca gcgaaacaga ttcttttgaa tttccagaag gttttcttta
    77941 tctacgggat tagctaaatg atttctgcat tcaccaatcc aggcatttag ttcatctaaa
    78001 gtgccgtatg cctctacccg caggtcatct ttatagaccc tttcaccgga atatagagat
    78061 gtttttccgt catctccact tttggtaatt atactcatca ggttatctca tcgcaattac
    78121 ggaaatctca attttgccat tttggggaag gcgcgaaacc tggacaactt ctctggcagg
    78181 ataaggtgcg ctaaaataac gcatataaat ttcattcagc agggtaaaat catccaaatc
    78241 ctgtaaaaat acactaacct taacaatatt tgacaaaccc attcctgctg catccaaaat
    78301 ggatttaatg ttattgaata ccaaatttgc ctgctcctca aaactttccg gtataagaga
    78361 agtgccagga tcaattccta attgcccgct aacaaataga atgttattca taagaatggc
    78421 ttgcgaatag ggacctattg cagccggggc acgatgcgtc ataactgatt gcattttata
    78481 ctccttacat ttttttacta tattataatt tcattactat ctgtcaagtc cgataaaact
    78541 gaaactcagt tttctccatt tttataacag ataggataaa ttgcttagaa tcaggtcatt
    78601 tttctttata tatatgttgt tatttactat aggagtaaaa caaacgaaca cctccttagt
    78661 aatttgataa aggccgaaat gacatatatt ttcccataat ttatatgagt taattaagat
    78721 attgccgaaa gaagccaaaa taattttggg gggatgcaag ttttatgaaa aagaacttga
    78781 cttgaattaa ctagattaaa ataaggatta tatgtatata tttatattga ttgtgagaaa
    78841 cacggacaat aaaatgtcct tgaacaacct gagaaactat tatttggaat ggacaccaac
    78901 atttaggacg catcaggttt aggactggaa gagaattaag acgatgaaag atataggtaa
    78961 tgagcaaagg tcagatgaat tgttagtcaa tcaagataaa acgctaatgg actttaatct
    79021 tcatcaatcc atagtaaata gcgatcttag ggatataaag cgagattccg gaatgttttt
    79081 tacgccagaa tggattgttg atttaatggt taacctgatt gatgatacga attatgttga
    79141 aaaagaaggc attaagattt tagaacctgc ttgtggttta gcacagtttt tattaggaat
    79201 aaagagaaat aaaccctcac tatttagtca ggcaaaactc tttggagtag aaataaatca
    79261 ggatatcatc aattatttat ctaacttgaa catagcaaat gctattgatt tgaatagggc
    79321 tgattatctt ttatggcaaa ctcggtcata ttatgattta attattggta atccgcctta
    79381 tggtattcct agtttatctg aacactacac tatcaaaaca aatcctgcaa ccaaggagaa
    79441 atataaaaaa ctttatgcaa catggtttgg gaaatataat gtttatggcg cttttattga
    79501 aaaatcaatt aacttactta aagataacgg acaattgata tttattgttc ctccaacctt
    79561 tatgattctg gatgaattca aaaaactccg agtattttta gctcaaaacg ggaagacgga
    79621 aataatctat ttaggagcgg atatatttaa accggaagca gatgtttcat cggtagtgtt
    79681 gaattttatt aagtccgata aatacatctc ccggctggaa ctgaaagaat ttcaagataa
    79741 taaaatcagg acgctaaaaa gtaattttca ctggaaaggt gaagttgtta agtttgacac
    79801 tgattataca cagacattgg aaagcttatg tccatatagg ttaggagata tttacaatat
    79861 tagaatttca cccagaactc ctgaaataaa aagaagtaac attatagtga aagaaaaagc
    79921 acctggtgaa aatgattatc ttcctttatt aaatggaaaa aatctaaaat gtaatatgat
    79981 aaattataac aatcttactg gttactgggt taaaaagcaa gatgtaaaca aaatcagaat
    80041 gtattttggc tcccctcata tcgtagtggg cttgggtttt agagaaaatg gcaaagttgc
    80101 cgcggctttt gatgaaaaat gttatccctg gatgggtgat gtttatcatc ttgtaagaga
    80161 gaacagttta tttaactgcc attttgattt ggaaacagaa gaagtagttg catacttaaa
    80221 ttctgattat ataaaaaagt atgtaaaaga cacttaccgg gaaattactt atcatcttag
    80281 tattactcag ctaaaaaatt tgcccgtacc tgataaaaat actttacaac ttattaaaaa
    80341 ggagaacctt tgtgagtgat agaataattg aagatttcaa gagatatcgt gattttcttg
    80401 taaccattcc cctggataaa tatcgcgaag aattaaaaga tattaaatgg gttgaacagg
    80461 atttgcctaa agagatacta cctctggcat caatatttaa atattactgg gaagaaagac
    80521 aatttctgga ttttgaagaa tggtttgata ccttttggaa agagattaat actaattctc
    80581 caagtaaaga agtacttaac caatttaaga aatattattt tgataaggac aataacggct
    80641 ggttcaaaaa aggttttaga gccagaatgt atagaacttg ggtctctgtg ctcactcaat
    80701 tggatttttg ttatttattt cagtttcttt gtgcaagaga aggtaagatt ttatcgctgg
    80761 aatgtaatgc tgctttagat gccagaggag ttgacgctaa ggttggaaat atcggatttc
    80821 aagttgctaa aattagtaat agaaaagaag cacgtagtgc cagtaaaaag aaaacattgg
    80881 taacaatacc ttatgcagtt tttaatattg aagaatatga aaagaagatt ataagcccaa
    80941 ggggaaaaga taaatcgggt taccaaaatt cactgaaagc atttcaaaaa tattttatcc
    81001 gtctcaaaaa tggttttgtt gttttcggag aagcatacct tctacctatc atagataaca
    81061 tagaggacat cacaaaagta aaagagacgg tagataaaat atctttggaa ctatcgggtg
    81121 aagaagtgta aatatgattc ataatggcaa ataatagttt ttagcaaatg ataaagttaa
    81181 caatctcaga ccttgtgaga tcatttatat gcagcgtgat tagacagaaa agttatagtt
    81241 tacagcccct aaatatcata actaattaac ttcaggcata aagcttattt tcaatatctc
    81301 ctttccttga tttagagcaa aattatttag aacgacacaa atccttatat cctaaaatcc
    81361 ttgcttcaaa atctatctgt gtaatatgtg agaggttcta aaatcttatt gccaagttgg
    81421 tctatttgat ttatctttaa tagtgaagga cttttgagga gaaaaaatgg ttcaggaacc
    81481 acaaagtttt cacgaattta tgccagataa ggtctggaaa tggaaaatcc tggaagaaaa
    81541 agtggaaaag gttttatcct tgcacgactt tcaggagatt cggctttcgg ttttgcagga
    81601 tagcaaggtt ttagaagatg gcataaccgc tttaattcaa ggtcaggaag ctgatactgc
    81661 aatgaaagga gttctttctc tttcccttcc tgatgatcaa ttaagtttat tgagtttgcg
    81721 tcctgaaggc acaatcagtg ttttacatca tacagcgcgt attaccaatc cgggtgaaat
    81781 tcaccgtttt tactattcgg gtcctatgtt tcgccgaatt aacaatcagc aacagatgga
    81841 attttatcag ttgggagtgg aacttttagg cagcgaaagc attctttccg aaaacgaagt
    81901 aatttcttta ggaatgaagc tttgtaagac actgggtttg aatgaagttc gccttgatct
    81961 aaattcttat ggctgtttta cctgtcggga gtcatttttt gcagacctga ataaatatct
    82021 ggaagagcat aaaagcgaat attgcaattc ctgttatcgt gagctttcta ccaatccctt
    82081 cagtcaagtt cattgtacaa atcccgattg caagaaatct cttcaagagg gaccccacat
    82141 ttatgattac ctttgtaatt cctgtaaaac agattttaat aaagtgaaaa acattcaggc
    82201 aaacctggga aatcaatata aggttaatcc taatcttaac aagaactttg cctattacaa
    82261 taaaaccgtt tttgacttta ttgctaaaca caaaggaaaa gaaattatta ttggcggtgg
    82321 cggacgctac gattatcttt cagcacttat tacaggcaaa agaattccag ctgtcggttt
    82381 ttatctcaat ttggacagca tttttgaaat tatggaaagc cggggcttat ttgaggaaaa
    82441 agaccctgaa tttactgttt atatctgcgc tgaaagtcca aacctggaaa tgatgaccct
    82501 gcaaatagtt caggaacttc attcacaaca gattaaaacc attatctcac ctgaatgcag
    82561 acccgcagaa aatgaaattg ctattgccaa aaagaaggac tgtcccataa tgcttatttt
    82621 gcgtgatgat aatatccgcg aaggaaaaat cctgcttcgg aatctggtga aagaagaaca
    82681 gaaatatatc tctttaaagg atactctgcc ttccattatg ctggcaaaaa aagcaatgca
    82741 aagctaacaa attaatagaa ttcagataac cgaatctacc gaatgttggg gataataaat
    82801 ttgtttcccg cagattacgc tgattttatt gcttacgatg ctcactgttt gtaatttaag
    82861 cacttgatgc ttcaatccaa ttttaatata aaaaggtctt atatcttgct taattatttg
    82921 tggtagagat gtcgtggtgt ctctcggcaa atttccgata gacgccattg tattatgacc
    82981 atatataatt aaggaagaac taaccggaac ttccttattt tgcgtccaca ggttagattt
    83041 taggttgcag aataacaagc ggagttacaa taagttataa gaaagagggc tggcatccaa
    83101 ttgtaagcca gccctcttag ttaagtaact tatattgcag gttattgcgt tataactaag
    83161 ccctgatggg gttcccaatc cagcataatc ttctttccgc tggtgaattg ccctgccagg
    83221 atatattttg ccaaagtatc ctcaatttct ttctgaataa ggcgtttgag cggtctggca
    83281 ccgaatgccg gatcatatcc cgcttctgcc aggtgctcaa taagattttc tgaaaaatca
    83341 agctggagat tgcgatttgc caagcgggat gctaaaatat ccaattgcag tttcacaatc
    83401 ttttttatat gatccttatt caaacggtgg aaaagaataa tatcatccac acggtttaag
    83461 aattcgggac ggaaatattg ctgtaagata ttcatcaagc gcggttttat ttgttccaga
    83521 tcactgtccg acgcttcata aatttcctgt gaaccgatat tactagtcat aattatcacg
    83581 cagtgtttga aatctaccgt tctgcctttt ccgtctgtaa ggcgtccatc atccaaaatt
    83641 tgaagcagga cattaaaaac atccggatga gctttttcaa tttcatccaa aagtagcacg
    83701 gaataaggtc tgcgacggac tgcttccgtt aaatatccac cttcctcata gcccacatag
    83761 ccaggaggag ctccaattaa tcgtgctaca ctatgttttt ccataaattc gctcatatcc
    83821 aatcggatca aagctttctc ggtgtcaaaa agataacttg ccagggtttt tgccagttcc
    83881 gttttaccaa ctcccgtagg acctaaaaag ataaaggaac cgataggtct gttttcatcg
    83941 cttaatccac tgcgactgcg gcggatagca ttagccaatg ctttaattcc ttcttcctgt
    84001 cctacaactc tttgggcaag aacattttcc agttctatta gtttttgcat ttcgctttcc
    84061 gccagtttgg aaacaggtat acctgtccat ttggaaacaa cttcggctac tacttcttca
    84121 tccacaattt ctttcaggag gcggtccttt ttggtcattg cttcatcctg cagcaatttt
    84181 tgcagttctt tttccttagc agcgattgtt ccataacgca gttgagcggt tttttcgtaa
    84241 tttccagcac gttctgcttt atcagcttca cctttaaggg tatcaatttc agttttaaga
    84301 gtgcgaattt gttccagcac ttgtttttcc ttttcccagc gcaggcgtaa tgctttgttt
    84361 tcttcgttca attgagcaat ttcttccttg attttttcca ggcgttcctg ggaaagaggg
    84421 tctttttcct tagccaaaga aaggcgttct atttccaatt gacgcagtcg tctttccgat
    84481 tcatcaagct ctacaggtaa ggaatcaatt tccattctca acaaagcaca tgcctcatca
    84541 atcaaatcaa ttgccttatc aggtaaaaaa cgatcggcaa tataacgctc ggataactgc
    84601 gctgcagcta ctaaagcatt atcgcttatc tgcactccat gatggacttc atatttttct
    84661 ctgatgcccc ttaaaatgga aattgtatct tccacggagg gttctgtcac taaaactggc
    84721 tgaaaacgcc gttctaatgc gggatctttt tcaatgtgtt ttctatattc ggttaaagtt
    84781 gtagctccaa tgcaatgcaa gtttccccgt gctaaagccg gttttaacat attggatgca
    84841 tctactgagc cctcagcagc tcccgctcca acaaccgtat gcaattcatc aataaaaaga
    84901 ataatgcgtc cttcggattt ttccacttcc gaaagcacag cttttaagcg ctcttcaaat
    84961 tcgcctcgga attttgctcc agcaactatc gctgccatat caagttccag taattccttg
    85021 tccaaaagat tctcgggcac atcacgagct acaattctgc gggctaaacc ttctactata
    85081 gcggttttac caactcccgg ctcgccaatt aaaactggat tgtttttcct tctccgggat
    85141 aaaatttgga tacaacgccg aatttcttca tctcttccaa taacagggtc cagcttttcc
    85201 attctggcta aacgggttaa attgcgcgca tatttttcca atgcctgata tttggcttcc
    85261 ggattttgat cagtaacacg ctgattgcca cgcaattctt taagtgctaa aagcagttta
    85321 tcggcttgta aaccggcatt tttcaggata ttagctgcct ccttaccctt ggctaagatt
    85381 gccaaaagca aatgctcagt agaaatataa tcgtcctgaa actggtctgc ttccttttct
    85441 gcctgatgta gtatgtccaa gacctcctga gataaatact gctgtgaact tccacttacc
    85501 ttaggaagtt tgttcaatgc attttccact tcgcttttca ctttgggaat agaaacctca
    85561 agtttttcca aggcaggttg aattagccct tcttcctgag ttaacattgc ctttaacaaa
    85621 tgcaggtctt taatttcctg atgtccataa ctggaagcta aatcctgcat atcagcaagc
    85681 aattcttgtg actttattgt aaatctttgt gtattcataa ttacctccaa ctgcaagaaa
    85741 atataatcag cattacctat atgtcaagta aaaaattagc agtcccctgc c
//
LOCUS       DAOOZV010000002        56813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_645_length_56813_cov_61.037686, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000002 DAOOZV010000000
VERSION     DAOOZV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 56813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56813
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_645_length_56813_cov_61.037686"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PLW08_00415"
     CDS             complement(<1..1026)
                     /locus_tag="PLW08_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPL51102.1"
                     /translation="MNIPINVYVAYFEKGILNFGYVIAQKNNRLEVITETGTYVVLPE
                     SRIILQSKEKYLESEPYQALPNFINQVNLFEEQFQESDFRFLKEKECTLQEIATELNL
                     QTDVQIFALFKYLHNHPEKIHCKKNKYRLKTPEEITQYQLQLQQQEEERQFLQDVNAF
                     FSGAELSRESQHKLYNALPELQTAKKYKKLKELILSQDPLLKPEEAILEFRKFCGETP
                     EYIDPVIANAGIPIGFSSLLIEEKLLPWKATQPEAIAFSIDDESTKDFDDAISFTKDG
                     SFWHLTLYVSSVSERLNLEGALFAEAKKRVSSLYTANAVIPLFPFNHSEQELSLKKDS
                     VRPVLALN"
     gene            complement(1052..1127)
                     /locus_tag="PLW08_00420"
     tRNA            complement(1052..1127)
                     /locus_tag="PLW08_00420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1092..1094),aa:Arg,seq:cct)
     gene            1227..2243
                     /locus_tag="PLW08_00425"
     CDS             1227..2243
                     /locus_tag="PLW08_00425"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPL51103.1"
                     /translation="MLCQIDKGKQVKMKVAIVGATGEVGRMMITCLEESNLEISELDL
                     FASSRSAGTVLYYTDQPIKVQELKQDSLLKHYDYVLFSAGAGVAKTFAPIAIQTSEVV
                     IDNSSGFRKEPNIPLVVPEVNGYLLTGYSGIVANPNCSTIQIILPLAVLDKLFNLKKL
                     IVSTYQSVSGSGHQGIVTLLNQRKGKKDKGIYPHIIDLNVIPQIGGFLDSGYCQEEEK
                     MHFECRKILNKWDLLVSATTVRVPVIYGHSVSVYAEFENEVDIPKAEKTLQNSPRIVY
                     YPNTYITPLDLGSSNETHICRLRLGTDEKSLTFWNVGHNVRLGAAANAVNILITHSKY
                     SGRL"
     gene            2243..3364
                     /locus_tag="PLW08_00430"
     CDS             2243..3364
                     /locus_tag="PLW08_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPL51104.1"
                     /translation="MIDIIELRHSLHRIPELAFQEFKTKAMLEENILRLLQNQPENLW
                     HLHYFQNSPGLLLEYSPAKGNYLLFRADMDGLPITEKTGVPFASEHPNLMHSCGHDVH
                     LSILMGLIQYVANTLPQRNILFLFQPAEEGEGGAKSILAEGIIQKFEITSVFALHIAS
                     ELPVGSVSAKEGIFFSISQEFDVLFYGKASHIAFPEKGIDALSAGIKFMNTIEDKLAE
                     LKKTEKIIFHIGKMSAGTIRNVIADKCVLEGTHRSLSPKISQKINILIRDTAELIARN
                     TNTRSEVKFLASYDPVINNPDLVNRLQDICRQQNIEYIPAQTSMTGEDFGFFTTLYPG
                     LLFWLGSGCSYPLHSDKFLPQDECLEIGVKIMSAFLTTE"
     gene            complement(4161..9614)
                     /locus_tag="PLW08_00435"
     CDS             complement(4161..9614)
                     /locus_tag="PLW08_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51105.1"
                     /translation="MKRSIALILILLAGCIVFANINDNLPANLNSAFTLGAKNSSSLQ
                     VNFTLPEYSMQEETYGGAVYHKIILPLSGTLMETGMPELPVVCTSIAIPHTGGVNIEV
                     LSTQQTVIPNFLPYPVQQGNSSESPKGFIINNEYYNSGNNYPEMLIEYSEPSILRDFR
                     IITIQINPFSYNAQTGELTVNHNIEFRLNFTQEQGINELPNEPANISASFDKIYDSMI
                     LNYGDYRDAVVTNTPPRYLIIYGTNSDTNFTSALNEYVLWKKQKGADVMVASTAANEA
                     GNTTTSIKNYIQTKYNNPETRPDFVVLIGDTSGSYPIPAYSAANSGVTDYNFTFLAGN
                     DQLGDCFIGRISVENLSQFLVVLNKIYLYERDINLATASWLNKMMLSGDNAPSGISTM
                     YIHKYIKEMSLLTNPDYTFTEDYGASPNYTIINQALNQGVGFYSFRGYIDWSPPAESA
                     LFNGYKLFHTVNITCGTNNYSGAGEVEGMIRYGTTAAPKGAVTGIGMCTSSTHTTFNN
                     AIHGGIFAGIFASDMRTMGEALLNGRLYLHQLFGVSSPTNATNFAAWCNLMGDPTMEV
                     FTGIPNHFDVTSDISIPLGLKLYDVAVQDNSGNPVEGAAVVLNMGGLILARNYTGADG
                     TAILVLPSDMLAGNATLTVSKHNFKPIQTTISVVNMATLVPGTMVIDDDNIAPSQGNE
                     NGLASSGETIELFLGLTNTGTDIITGISGSVISNSPYITIANSSVIYPDILGGETGNN
                     SVPIIIQIAPDTPHQTMLRLYLNLTDSNSVSYHISEFIPVEAPNIDFVSYLVMDSNNQ
                     HLDPGEVAEFSITVKNTGTVEADNVLGILYTQNDLVGVSDPNAEFGSLPTGILVTCGT
                     NRFTLSARTEVLPGMLIPMRLKLYNSDGFEQFVDFTFTVGVVTQQDPLGPDTYGYVIY
                     DWSDTNYAEAAVYDWIEISPLEGGLGTTLAISDGYASGNEGDQVGSDALEVVSLPFPF
                     QFYGILYNQITVCSNGFIAMGVTANAEFRNYRLPGPMGPNPMIAPFWDDLATGTGSGI
                     YTWFDRNHHSFIIEWYKLKNGNGGTALETFQCILYDQAAYPTSFGDGPIKFQYQTFNN
                     IDSQSGNKHGNYCTIGIEDHTGKVGLEYTFNNTYPTAAAPLSNGKALFITNVPIYHSS
                     AHLLLEATYVNDTNMNGICEPGEIVELGAKIQNTGNLTAENVIGQISTASEYVTINNA
                     TAEYYPIEPGMNGVNKEPFTFTIANNCPDGEVLYFELLITSGELQWTRNFSLQVSASC
                     LLYHSFFVDDHLNDFDGMVSSGEFFNLIVNLENFAAVDATSIQATLSSPLQQLIIEEP
                     LLQFDKIGPNDIYQIKFLINTSMLDVSVTQIPLLFTATASNGSSTNASFSIPYNNPVV
                     FQDFELNNGYFVSETGWVWGNPSQVTPFSGAKVWATNLSGNYPSYVNYNLYTPKYLLE
                     TNSQLQFRHYYSCEYGNDGGNVSISTNNGVVWNLIYPVSGYNGYSLNGLNGDPGWTGN
                     SDGWQLAIFDLSQYAGQSVMFRFRFGSDGEISGPGWFIDDFTVSGVNLKTGYLYGVVY
                     PSSGLDPSMATLRSNQRFTSHPDSEGNFIIFLPNGIHTVTATMPYHQSSTLTSIIINP
                     SNPVHYTEFTLIDLPQPQSISYIGNNDTGDLYVFWQPPEDTVLPIVGFKVYRKFNTDQ
                     FYLVQDSPEISYHEVLTLPGHYKYYIVVKYLNTDGTPSQILNIPFPFIPNSDYEIPAL
                     VTKLNNNYPNPFNPTTTISFTLAKPGKTTLTIYNLKGQQIKRLVNEELPSGMHNVIWN
                     GKDEKGRNVASGVYFYRLQSGNYQATQKMLLVK"
     gene            complement(9680..11101)
                     /gene="glnA"
                     /locus_tag="PLW08_00440"
     CDS             complement(9680..11101)
                     /gene="glnA"
                     /locus_tag="PLW08_00440"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPL51106.1"
                     /translation="MELKELQHLILANEVKAVDLKYCGLDGKWYHITFPARNIAEVME
                     VGIPFDGSSIPGMRSVESGDMVLMPDSKTAHLESFYETPTLRMICSICDAETRLGVKK
                     DPRSVALRAHNYLLSTGIADMSTWIPELEFHLFDSVQYCSDSFSAGYNITSSECKEAL
                     PDDNEDPGSLSLQGVKGYHIDTPFDQFYEIRQKIVELIEEQDIKVRYHHHEVGISAQE
                     EIETELLAFPKICDDVMIMKDIIRKTALQYGLTATFMPKPVFGHAGNGMHFHIMLHKN
                     GKNLFYKKGGYADLSEEAIWFIGGILIHGRALVAFTNPSTNSFKRLLPGFEAPVKLFY
                     GLANRSAAIRIPKYANTPETKRFEFRTGDGTCNPYLAMSAILLAGLDGIKKQIDPAKY
                     NLGPFDDNVFAWSEDKKAQLLSIPTDLKEAMQALKEDHQFLLEGDVFNEELIESHIEL
                     KLKENEAVSARPHPHEMLLYYNL"
     gene            complement(11496..12143)
                     /locus_tag="PLW08_00445"
     CDS             complement(11496..12143)
                     /locus_tag="PLW08_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPL51107.1"
                     /translation="MISPNIIKLLVNAVIIFLVFYSIIIHEFSHSLMAYWLGDDSAKR
                     AGRLTLNPLKHIDWFGTVILPLFLYLTCGFIYGYAKPVPFNPYNFKNLKRDSGLTGLA
                     GPLSNFLVAIFFALIFHLCGFSVVIKYICFFVIYLNLLLAFFNLIPVPPLDGSKVIGM
                     FLTDQAYYKWTMQERKGMMYLFAFIIITTLLGWNIIGKIIIPPVNFVMQIMGVRI"
     gene            complement(12140..13342)
                     /gene="tyrS"
                     /locus_tag="PLW08_00450"
     CDS             complement(12140..13342)
                     /gene="tyrS"
                     /locus_tag="PLW08_00450"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPL51108.1"
                     /translation="MAFEQELKLISRGVEEIITLEELKAKFAKAEKTGKPLRIKYGID
                     PTGYDVHLGHLVPIRKMRQFQDLGHIGVIIIGDFTAQIGDPTGRDESRPPLSAEQVKR
                     NSEKFMEQLYTVLKKEQTEVRYQSEWFGKMGMSDVIKLLGKFTLAQFMAHQTFRQRYE
                     QGLALGMHEILYPVLQGYDSVAVNSDVELGATEQKFNILCGRDMQRHFGMEEQVAILS
                     PILIGTDGVNKMGKSLNNYIAVFDPPTEKYGKVMSIPDNIILNYFYYATTMPEEEIEK
                     IKKELESGTNPMLIKKRLAREIVTLYHSAEEAKEAEYAFEKQFSLREVPNDIPEFVVS
                     KNSCKLSNLLVDSGICSTNGEAKRLIQGGAVSLNGEKITAFDAVVNLKDGDIIRAGKR
                     NYIKIKKI"
     gene            13724..14194
                     /locus_tag="PLW08_00455"
     CDS             13724..14194
                     /locus_tag="PLW08_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51109.1"
                     /translation="MALVMGKQCCNNARWKKLLYPENSGLYLIPIMQLKKNGREKKEK
                     KSGLLFYEGLTHPSLLLCRPQGLFFRGKEKKEKKKGLLFYEGLTHPSLSLCRPLGFQK
                     ITTFSILSTLSTLSTFSTFSTLSTLSTLFYEGLTHPSLWKFRPLGFQTYYINWD"
     gene            complement(14348..15445)
                     /locus_tag="PLW08_00460"
     CDS             complement(14348..15445)
                     /locus_tag="PLW08_00460"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPL51110.1"
                     /translation="MKKRLLIATGGGDCPGLNAVIRAIVKRASFEHNWEVLGSIDAFN
                     GILKDPMHIIELTPEKVAGIHIQGGTIIGTTNKGGPFNWPIKNPDGTWTTVDRSQDFL
                     DRLRYQNIEALINVGGDGSQRISQQLFELGCPVIGVPKTIDNDLSATDFTFGFQTAVD
                     IATDAVDKLVTTAASHHRILILEVMGRFAGWIALHSAIAGGAEVCLIPEIPYNIKNIM
                     QAINERIDRGHDFANIVIAEGARPVDGDMTFVECDEPGAMKIKLGGAGLRLAQELKNA
                     GCTIDIRVTILGHLQRGGTPNAFDRILASQFGVKAFELVLQEKWGSMVAYRHPNIVAV
                     PITEAIEKYNYIDLDSDLVATARGLGISLGD"
     gene            complement(15495..15917)
                     /locus_tag="PLW08_00465"
     CDS             complement(15495..15917)
                     /locus_tag="PLW08_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL51111.1"
                     /translation="MLSIKRIKELNKKIYEEVSTLWEKTGINKPERKDSFESLQENLQ
                     QTGILIIAEENEKVVGVVWVTNDFRRLYIHHMAVVPNKQNQGIGTKLMEECISLAKEY
                     GYQLKLEVNVDNPAAWHLYEKFGFKELSGYRVLIKRDI"
     gene            complement(15919..16842)
                     /locus_tag="PLW08_00470"
     CDS             complement(15919..16842)
                     /locus_tag="PLW08_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPL51112.1"
                     /translation="MKKSKLILLLVLLILIGLYFVLRTNRPQEKLARVFDLDTLSINR
                     IEIYDAQDTLKMVKQNNIWKLIYPVNWEADSLKIRDLFREVITAKYAKTPMGTGKEAI
                     EKFHQKDEEALHIIVSDGKKSIHTLFSNLDNPYDYFRYAGSDKIYQLKAKVTNNYNTD
                     LSNWRSPHIVSHKEEELLKIEVTHPKNKYTLTRNGLQWTFQDAHNEFKIHPANRAMLK
                     ILNILESLNTYIFIDNAQAEYAEKFKNPYCTVKLYLTGNRTQELKFIEHTQGYYLMMV
                     DNDPSVLFVVVWDTVFRFTRHPDIFRMQEFG"
     gene            complement(16829..18403)
                     /locus_tag="PLW08_00475"
     CDS             complement(16829..18403)
                     /locus_tag="PLW08_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPL51113.1"
                     /translation="MKKEKKTSSILSNLLIKLAIVLMVLLIGSYARVRWDFSKNKAYS
                     LSQVSKDAVSNLQDNMVVKLYSSKELPAAMLILDRYVKDLLEEYKQAGKGKFHYEVIN
                     GPSPEDLKNKALQYGVGAMYFRIFENDKTTTKEVIYGLVFEYQGNFESMNVLPKMQNQ
                     LEYQMTLKIQKITRYTLPDITAFVDTLYTLMPRQKYENELYSNYNVHFTNLLEPPPKT
                     PVMIVHSGYDSLSITQLYNLDQFLMQGGKLVVLADKIFSDGETVMEIHSNLFDFLENN
                     GIKLSSDIAMDIFCDSRQMGVDTNISFPIYPVLRGSQHPITRNISNIIIYMGNGIIFN
                     RKPGLKFQTILATSTRSALLEGPDYQLDSRLFFNPDPDVFVNPPIPLGAIVEGRMESY
                     FKEKPQFQRSDFVSAVKDGKIVIFGDRELYVDPDKPIYENRYQIILNAVDWLMGREDM
                     INIRSRHLQESILDIAYYMHKNNLLWGDPAKKERRIKMEVKIIATLLPSLILIAVGGI
                     KSLHRKRTKEFKNEEI"
     gene            complement(18405..19127)
                     /locus_tag="PLW08_00480"
     CDS             complement(18405..19127)
                     /locus_tag="PLW08_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51114.1"
                     /translation="MKTVFTIAKKEYELALHSISTYIVYILFLVIIGIIFSNMVFKIA
                     RAELRFLFEVIHIVFLFYIPAITMGSIAKERQTGTLELLSTLPIKLSSIVWGKILSAL
                     FQIITIIILSLVFFGIIVIFGEGIDYGAIICGYIGLILAGLAYASIGVFASSFPSNQI
                     LAFVLALLISAVFYLLKFLLPLLPFGLVPIVQYFSFDYHLSSFLKGVIDTRDILFFLA
                     VTVIFALLAQFNLQSKNLMQEK"
     gene            complement(19124..20221)
                     /locus_tag="PLW08_00485"
     CDS             complement(19124..20221)
                     /locus_tag="PLW08_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL51115.1"
                     /translation="MIKIDKLSRYFGEIKAVDEISFCIQDKEIVGFLGPNGAGKTTTL
                     RMIVGYLQPSSGNIEIDGESIFTDPLKASRQIGYLPEQNPLYDEMTVVELLAYFAALR
                     KLKRDYFRERLNFVVKNCGLKDVLYQRIGTLSKGYRQRTGLAQAILHDPRILILDEPT
                     SGLDPNQIIEIRELIRELGKEKMVLLSSHIMQEVQALCDRVVIINKGKIIVDDNIENL
                     PNYLKRSTMLILEVQGENVDFSDFLKEYPFVELDILSRNETTCKVCVYETSGGLISSS
                     ITNNESDKNSIDETSEFDLRQELSRFIRDRGWLILEFSTSKMSLESIFHELTIENYYE
                     PEIEEKLSAEQEFMEEEQTKPAETEDNREEQ"
     gene            complement(20223..21386)
                     /locus_tag="PLW08_00490"
     CDS             complement(20223..21386)
                     /locus_tag="PLW08_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL51116.1"
                     /translation="MRKKWWIIIVIVLLILLVLFLGKSCGKKKQTETVKPTETETYTV
                     KRGDISSRIEITGEVQPEVVVSLKSKVSGKIVKFYAGENDYVTSGQIIADIEPDYNQA
                     NTLFNTKAMLQKAEMNLKNARKDLADKTILLQKQFISQEEYDKASDALKEAEIEYTQA
                     SSQYEMIRDLDVPGKVTHVYATASGVVIERNINEGEMVQSSLTSFGEGTVIMKIADLN
                     KMIVKSNINEVDISKFKLNQSAEITLDALPYEQYNGKVVKIAPQAIIENNAKVFPIEI
                     NLNASGKVVKPGMTANVTILADSRQNVLVIPIRAVFSNDLNQDIVYLVTDTKSPSKET
                     KSKEKTSSAPNSVATPVKLGTNDLLQVEVIEGLKEGDIILLNEPVTNNNKMFM"
     gene            21678..22151
                     /locus_tag="PLW08_00495"
     CDS             21678..22151
                     /locus_tag="PLW08_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPL51117.1"
                     /translation="MNKLFMPELVKIVEKFENKNACLQYMANLLSESGCLSFPDRFLA
                     AVKGREEIMSTGIGKGIAIPHARDLTVSCLRIAVCLVKQDLDFSSVDNLPVHLVFMIA
                     VPQSSNQDYMKILRSLSEFLRREENRETLLQAKDERDLYEKVQFLEEELLPQLGV"
     gene            22099..23070
                     /locus_tag="PLW08_00500"
     CDS             22099..23070
                     /locus_tag="PLW08_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPL51118.1"
                     /translation="MKKFNFWKKNYFLSLAFSILFLMPLLAQHQDAGTTGFSTLKIIY
                     SARANGMGQAMLGRAKNFDGMQFNPASIIRVPNQGVFTTFADHFVGSGGGSVSYVVPK
                     NIYTSYGFYLNYWNSGSIDRTDVGTNGEFIELNDTFNAQDILAGFTLAKFVSPALDAG
                     GGIKFVLDQIDDKSASALLIDVGILHHTANERIKVGLSAKNLGFQISHYSDKKYDEGI
                     PFTYGAGFGIDVGKNTLLNIDITKANGENFIGKFGVEQQVHPSLVLRGGFKTNAEDYY
                     LGGNMEWTSGISLGVGWVWKKIALDYAVASYGDLGLTNQVSIRYNFE"
     gene            23103..25220
                     /gene="glyS"
                     /locus_tag="PLW08_00505"
     CDS             23103..25220
                     /gene="glyS"
                     /locus_tag="PLW08_00505"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPL51119.1"
                     /translation="MERSRFLLELGCEELPERQIEIACNGVSSSFATFLKSNKLYCGS
                     YKVSGTPRRIYLDALELDTHQEDEEILKTGPLVSVAYDEEGNLTPAGKGFLNRSQAKE
                     ATIFIQETEKGKFLAVKYIKKGLVTVELLKSWIPEMIVQIPFEKKMAWSQLGLIFSRP
                     LRWFLILWNHKPLELDFGGIPCGNITYGNRFLGLDKSITINKAEDYLPKLLEQKVIAD
                     IEERRKEIITQLAAIFPEGNFKVIEDRELVETVVNLVEYPTAVVGEFEEKYLSLPDKI
                     IISTINQAQKYFAVQDNQGKLSNKFVFISNGNPSASMIIRKGNEKVVKARLADALWYW
                     NEDTKYPLEYWTRHLDKVIFQAKLGTMADKTKRIMQLVSALADELKITEEQKKKAIRC
                     TELCKADLVTNMLGEKEFTKLQGYIGKQYAIASGEDREVAEGIFEHYLPRGSGDELPS
                     TLCGAIVAIGDKMDTVAGIIGIGMMPTGSGDPFALRRAANGIVQIISSRKWDLDLFAF
                     ADRALELIGKQTELDKKAKKNLHSFLEQRIIGLLKTNGIAYDVIESVMHIDKSHLHDL
                     ENRAQALNALKGKEDFIHLVIGFKRVANIIAETQDFVPFNKDKLVEPEEINLYEALQT
                     LHQDIDSALQNKDYPLALQYLIKFGKVIDAFFDAVLVNCEDDELRNNRHSLLKEVKKE
                     FLRVADLSLIVLETEQKEAGKCS"
     gene            25211..26215
                     /gene="pta"
                     /locus_tag="PLW08_00510"
     CDS             25211..26215
                     /gene="pta"
                     /locus_tag="PLW08_00510"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPL51120.1"
                     /translation="MFIIDELINKAKGNSGSIVLPESTDPRVLTAVAKIVAMDLAKVT
                     LLGESEIINQEAKALNIELNNVTIINPETSEDIPAFAEAYYQRRKEKGVTMAQAIETV
                     KNPLYFGASMVKEGKVEGMVAGAANITADVLRAALQVIGVMPGLKTVSSAFIIPVPDF
                     RGEKRIFLFADCAVVPNPSSEQLADIAMSTALTRKAIIGDEPKVALLSFSTLGSAKHE
                     LVDKVTMAKEILTERKVDFDFDGELQVDAAIIPEVAKRKAPQSSVAGNANILIFPDLQ
                     AGNIGYKLAQYIGKVEAIGPIIQGLAAPVSDLSRGCSAEDIVNTVAIVLLLADQQKKK
                     "
     gene            26240..27448
                     /locus_tag="PLW08_00515"
     CDS             26240..27448
                     /locus_tag="PLW08_00515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPL51121.1"
                     /translation="MAIKLSNRCRLIKPSPTLSLSAKAKEMKDAGIDVISFSVGEPDF
                     NTPEYIKASAHKAIDANFTRYTNNAGIPELRQAICEKLLRDNGLKYSPKEILVSPGAK
                     AAIVNSLIAVCDDKDQVLMATPYWVSYPYQVALANAEPVYIPTCEEKGYKIQPADLEK
                     AIKENPCSKVLIMNSPSNPTGTVYNQAELSAIAEICIKYNILVISDEIYERLVYDEAK
                     HISIASISEEMKQRTIVINGVSKAYAMTGWRLGYAAGPGDIISAGGMVQEHTTSCVNS
                     ITQYACVTALKEEDDSIEKMRVEFSRRRDFLYEELKKLPHITCFKPQGAFYIMPGIKW
                     YLENNNQNIKTSDDFCAKLLDKYYVALVSGNSFGMEGTVRFSYANSIENIKEGLNRFA
                     SFLAELASGR"
     gene            27451..27666
                     /locus_tag="PLW08_00520"
     CDS             27451..27666
                     /locus_tag="PLW08_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51122.1"
                     /translation="MNDFNDKMKERWKDKRKKAYNWPRLIIMVLALIIILYLMNHLGN
                     TKNVVQNPSASVTDTLQADTLQKEITP"
     gene            27663..28556
                     /locus_tag="PLW08_00525"
     CDS             27663..28556
                     /locus_tag="PLW08_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPL51123.1"
                     /translation="MSLVIVGSIGLDSISTPAGEIQNALGGSAIYGALAGSFFTDAYI
                     VGVVGTDFPIDGLNLLQKKGINLDGLELKEGKTFRWSGKYSNWNKVETLSTELNVFAD
                     FYPQLPESCKSCRSLLLANINPSLQMQVLNQVSGYSHLACDTMNYWIYLCPKEIEQVL
                     RRVNIVFMNEDEVREYTNKENIYSAARDILSLGPQWIIVKRGEYGSVAISNDDMYFAP
                     AYPLEEVKDPTGAGDSFAGAFMGYLANHNILNRAVIKEAILYGTVVSALNVADFSVKG
                     LIDKTKAEIDVNKEQLVKWTT"
     gene            28544..29590
                     /gene="purM"
                     /locus_tag="PLW08_00530"
     CDS             28544..29590
                     /gene="purM"
                     /locus_tag="PLW08_00530"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPL51124.1"
                     /translation="MDNLKNGLDYRKAGVDIQAGEATVLAIKQKVRSTYNSNVLSELG
                     GFGGLYKIDKNLWHQPILVSSTDGVGTKLLIAIMADKYETIGQDLVNHCVNDILVQGA
                     IPQFFLDYIGIGKLEPAKVKKMIEGIIIACQQNSCALIGGEMAEMPDLYEDEEFDLVG
                     TIIGIVEQEQILPRPKIKPGDQLISLPSSGLHTNGYSLVRKIVFEKLKLSLDSYIPEC
                     QTTLGELLLSVHRSYLPLMKDYLTDPNLKGLAHITGGGIIGNLKRILPADISAQIKLR
                     KTEIPPFFHWLQEAGKLSEETMRETFNLGTGMICIIDNEAFDKYLTIPEARCIGELVN
                     QNPTKEKVEFIQEC"
     gene            29788..>32123
                     /locus_tag="PLW08_00535"
     CDS             29788..>32123
                     /locus_tag="PLW08_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPL51125.1"
                     /translation="MRKYALILLIIMLAGTVFLEAATTGKLAVRVRDNAGRPLEFVNI
                     VVMQGNQRITGGQTNAKGTAIIINIPPGLYTVKFSLIGYDTVVYNDVRIIVDQTTNLA
                     PTMNKSGFEMKAVTVTATEDKVDKNRVGSARNIEMSIVTDAAVSDVADIVALQAGVTN
                     VGGELHIRGGRSNEINYTIDGMSVTDPVDGGATLQVDTDAIKDMKVMTGGFPAEYGNA
                     QSGVINIVTKDGDPFFSGKVEYNTDHLIGDGRNTDVVKLAIGGPVLPFASQEMKEKFT
                     FYLNGAGEWLDGRLKDYYISDPNGDYTLNGRSLIDADYPIYNPYTGRDKILGIDIGNR
                     NYNSYNINLKTKYAFKPGQVATFAVRGDRSLNYPYSNNWRYALQHYAEDEIIQRQYIG
                     TYDYVINDKMNIKMKASYYQKDSYEGPKGIDRDSYIFMTVDPNNIPSDYIQRVALGDY
                     GYSTIDADRDGIYDYGFLPYDYWKFRLESVEEPRSIPGYLPPGYIYSAFIDDTTKILS
                     GRADFEWQINETHMAKTGLEIIKHNIKKNQLEDYLNIDEKRRVAYLNSIFDIRKYNID
                     NFVDADGNMLLDDEYIPEGVYAIVATKENPASIADLVPIYKPEDYFAAAKAASGKRDG
                     YKAEPWQMAYYLQDKMEWEGMIVNAGLRFDLWYLGKKYYVAQDDGSYALREFDNSDLM
                     QLMISPRLGVSHPITDKDVLRFAYNYQNQLPQMQYIFTSKTPEDAYASETTIRVGNPS
                     LEPQITVTYEAGLSHQLSDDYVLDLTAYYKNIYNYVNT"
     assembly_gap    32124..32223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32224..32679
                     /locus_tag="PLW08_00540"
     CDS             <32224..32679
                     /locus_tag="PLW08_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51126.1"
                     /translation="TFRIARGEEFFIPFTNFILPLDDFSANINWAYASGAPYTPQSYE
                     SGQELDTNSKRMPSTQQTNLRITKGFLLSNKINIKVFAEVENLFKNKNIISVYPKTGN
                     YYWNGSQIEESNIPGFVYPEVYYVNSLASKNPLYYNDFRGLTLGFSFNF"
     gene            32706..33467
                     /locus_tag="PLW08_00545"
     CDS             32706..33467
                     /locus_tag="PLW08_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPL51127.1"
                     /translation="MRKFIGTMLLIMMMTFVFASFAYAQEKPAAETAGGGLQNFAELV
                     FGSGLVKWFKNGGWAMWAILFLAIYGLAYIIWKFIALLNGKVNLNNFLNKIIPLIKEK
                     KFEEAKEIAKNTRGPVAAIIYAGLEKVDKGLTAVDEAIENASIIEMSYLEKGFIELSS
                     SITLAPMLGFLGTVAGMITAFDAIAAARAVDATIVATGIRIALITTEAGLIVAIPVQF
                     FNNVFMTMVDGIVLDMQRASDKFTEVLTEELGLAR"
     gene            33464..33889
                     /locus_tag="PLW08_00550"
     CDS             33464..33889
                     /locus_tag="PLW08_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPL51128.1"
                     /translation="MKIVRKRKYRATIPTASTGDIAFLLIIFFMSTTKFDVKEGIKLV
                     LPQAAESQENKTKTLTLTEKEMTRFQIFPDGQIAVNREAPRYIDSEELDVIIQQKVQI
                     NPDMIFKVITDREAKYNDMIRVIDRLKAAKIEKISLSTS"
     gene            33898..34335
                     /locus_tag="PLW08_00555"
     CDS             33898..34335
                     /locus_tag="PLW08_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPL51129.1"
                     /translation="MAKFSGKKRRDVVIPQASTSDIAFLLLLFFMVTTVFVQEKKFWV
                     ERERWPFAESIERIPRNHTSTIYVMRDETILLDDIPTRIESSEDVFVSQTLIRKKEED
                     PELIVCFRTDRETKYGVMSDIMRQLQDANALVVAFETQPILKK"
     gene            34346..34981
                     /locus_tag="PLW08_00560"
     CDS             34346..34981
                     /locus_tag="PLW08_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPL51130.1"
                     /translation="MNTKNQDWKDIANAQFSKALALSLTLLIFGIMVTPKVEVRKQVF
                     QSQQMELVDIPPEERERIEAPQTEVDVNVTFQISDVLGTEEVDMAAFQEALSQIGNIY
                     STTAPTQQQGEDDTPVNFVPYDDAPVVIGKIEPVYPEFAKRNKLQGTVILEVEVLKDG
                     SIREIRVRRGVGGGLDEAAIEAVRKVKFQPGKSSGQPVDCMVIIPVEFKIQ"
     gene            34995..38117
                     /locus_tag="PLW08_00565"
     CDS             34995..38117
                     /locus_tag="PLW08_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51131.1"
                     /translation="MKFYKIGICLCLLLFFTASAAYAAMAVPATGTKKLDLTKYHNVG
                     NIWLRVSNYGFFGSGDDIVPQYPSLEYPGGSGIDYLYQGALWFGAKKQRRDSFGKKLY
                     WLVYPPSETNDTIITDADPRWNSTKKAVIDTLVSVGFDGDDDLYEFLPAYNPLLVNNP
                     DQTENYSTYNNLDGIIYATTRKQKRGVDDDGDGKIDEDGAGFTFPFRSGSELPAQFSE
                     NFGSHFLAEVPIRDFTILDDPINAEIWFPLGFMDLSYINSNYAFASPYDDDQDGRIDE
                     DGAPVSEQDFISFYYDYCPFGTSGERDYGKSSGANRHYPLNIRVRQMSYQWSFEYIKN
                     LVYVEFDITNMNEADTLYDCAMGIYMDCDVGPQTWGVEKASDDKSGYVKGTGYEFAYT
                     YDADFDNGITPGLVGARVCTPDPEKLKFHCWYWKVGDGPSDSNPIKIPKPGETSNEKY
                     WLLTGRNPKPSSSMYTALRPEQEDLTEWEQPTPNDTRFLFSFYGAQPENPSEYSDPAK
                     RWNLPPRKTMKIVVAVFPGDNKEDLKKSAGWAKEIYGQAQDLITVTQPDTFPHYTPPE
                     PPDIPNLYAQLQDDGNRLDVYWDNRSEFSYDVKTVSTAIIGWQNPESSLLISGLDSDP
                     TPYIANNWVDIPEEYRPGPPPEPGMEDKRWNMNALVNPYTASRLRKDFQGYTLWGRSG
                     SGSQEDWEMIKRWDKVDTPRDHSDYTVNSAFPDLFRDFGGYLGIDTGLPNKNEWNVDV
                     SEYHKFYHFDENYQLVPNGDTFYGWPIYTPDIVINGTTYNEVTDWQSLQNYANSIIGP
                     DLETTQLLQARIFKHQDIPDHIFYALYDPKLIPLQGFAFPGGEGAEAPDTLDLSNLRK
                     ERLARRYYKSYIMYPRKGVEYYVALTAFDRGIPANDLNYLESGRDADANMKVFFPGTL
                     AKENMDNIMVIPNPYIGRSSFDGRRENDEKGDKSRRIWFINLPKRCDIRIYTLAGDLV
                     QTLKHDGAPVTDIITISKASSTGLAADGMEAWDLLSKNKQIIAPGVYLYSVENKADGK
                     NKVGKFVIIK"
     gene            38128..39159
                     /locus_tag="PLW08_00570"
     CDS             38128..39159
                     /locus_tag="PLW08_00570"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPL51132.1"
                     /translation="MKKVLTLVLIITIAGLPLIITAKPFGKTGTVALQFLKLGVDARA
                     VGMAEAYTAVTDDISSVYWNPAGLAPAFENQVFVSHTNWPASIMHEFGAATYTNGVST
                     IALYGSILHMDDMDITEEEPFGPTGEKFTCSDIAIGAAFAQQFTNKFSAGLGIKYLRE
                     NLYEFNVNSYAVDVGSMYNTGWRNIKIGMAMRNFGPDIRFRVDDDEDGKDDEDPFDLF
                     DNDGDGLVDEDGIELDSKIPLSFSLGVSGDLMRKGNNYWIASLQLDNVIDRLETWNLG
                     TEYKLGNIFFRCGYQFNYDTNGFAAGVGWQVPTSFGIFNIDYAYTDMGDLAESFIKGA
                     HRVSLKIKY"
     gene            39189..41372
                     /locus_tag="PLW08_00575"
     CDS             39189..41372
                     /locus_tag="PLW08_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51133.1"
                     /translation="MKKILLVILTLSLLVGIVFAQEMIPTPDNKKAMETPVPKKIIPS
                     ERTAPVYTFTKTPHSLLTSYYDYMIGSYNGLPLRVIPSNIFGGGYFLTYHGRRTSTGT
                     RRIFYAYLDANGDVIVNNEITTEANNEGYGTVAVDPVSGKPFYAWHANSDTDAELEVQ
                     FTVDQFYEGIYGLFTPLVDAIDNPSTVYTSDSNFTDDNEFIWPTAVIGPSPYPDKRRI
                     YVIARNSVTHTYGPSENPKIAYADFDAFQIEQNVPLTWQYTSIPEMDEWNVDSEWRRP
                     FNSLTADNAGNLYYAGFHFATEADGSTDIVEPDVDVFQCGNYGEGTWLRTSSWGNLPT
                     WNPNTSPTDTTGFFKNQSELPYGDNDIYWELFSSASGHLNATVDDLGRIHFPAVWRLV
                     TNDGYWFPNFQLPKEIIFDPSLPEDSQFRINDIWPHKDPTNDVDEHYQSWDSQPPWGV
                     EDFVQDPDTGEWFIDMYLSWEFPYWDQNAHDSAMFFHCNNMKVTENNGHGMLAMVWQN
                     CARAKWYNADGDSDYSAWANTPEIWISVSGDNGNHWSEPIKLNNIETPEFAGIKPMWV
                     YPADKVIYVGESNGEKVGKLGLLFYDDFTWGAFVISPGVGPMNDGGRVMFTELQIVFP
                     SNASDDPTVSPVTNMLNQNYPNPFNPETTISFDMPKTANANLSVYNVKGQLVKTLHNG
                     IANFGRNTVVWNGTDNNGNKVTSGLYFYRLTTDGKVETRKMMLMK"
     gene            complement(41612..42535)
                     /locus_tag="PLW08_00580"
     CDS             complement(41612..42535)
                     /locus_tag="PLW08_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPL51134.1"
                     /translation="MKKILILILWFLIGVSIIIVYLTLGKPDTNIITQVRIPRLLLTL
                     FTGTALAGIGSIYQLMLANPLAEPYILGISSGSAFGSILLGISGLIILMPVGGFIGAG
                     FTMLLVWKLAHQKGRFDKSRLLIAGVIAGMFFSAGISLIMYLHQQDTALILGTLMGNL
                     GHIFTKGEWTSFLILMGLSLIILIYLFFQSTAIDILSCGDLYAASLGINVLSLRRKIF
                     ILTSIVIGISVSYAGIIGFVGLIIPHIVRFFIPSGQKRIYLLSLWSGGIFLLACDLLA
                     KHILSMELPVGIITAFIGCPFFMWLLLKKQG"
     gene            complement(42532..43377)
                     /locus_tag="PLW08_00585"
     CDS             complement(42532..43377)
                     /locus_tag="PLW08_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPL51135.1"
                     /translation="MKKSGILTVIIIILFAFSCKQTGNNQKKGIVVLSPEVAEIICAL
                     NAEKEITGITAECNYPPSLQQKKIVGSFSSIDKEAIIALNPELIFCSSLEQENIAYDL
                     KNLGFRVEVIYPKGINEMLSDIERIGTLIRKKEEAVKLVNYLKEEIEKIKANAQGKLR
                     PKVYLEIYRNPLMSVSDSSFIGELIELAGGDNIFSTLERDYARINPEAVIAAKPDIMI
                     CYSQDTLKNIINRKGWQNIPAIQKRRIYFEKDINPDLLQRATPRCIEGMKELAKIFAE
                     WREEK"
     gene            complement(43821..44141)
                     /locus_tag="PLW08_00590"
     CDS             complement(43821..44141)
                     /locus_tag="PLW08_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51136.1"
                     /translation="MEIPNRILIIRKVTLLNVSRTEPPTMICVRDAINTKLISRPIIN
                     AMESHNLLEVKIIHLFIKLNLLYLHHSNYYNKHLTKLQEKIVKPKSRKNTNTFFPYRL
                     AIRI"
     gene            44143..45540
                     /gene="cls"
                     /locus_tag="PLW08_00595"
     CDS             44143..45540
                     /gene="cls"
                     /locus_tag="PLW08_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPL51137.1"
                     /translation="MLIILIVVMENGSPATTMAWILVLIFLPVVGFILYLFFGRNWRK
                     KRLFSKKGYADTKLLLSYVHNHPSDRENNWQNDLTARLYNLLKNNSKATLTVNNNLTL
                     YNDTGKAFKAILEGINSAQHFVHLEYFSVFADETGYTLQKLLIQKALEGVEVRFIYDD
                     VGCWMLKKSFKQELRDAGVEFVPFMPVWIPFLNSRLNYRNHRKLVIVDGKKAYLGGMN
                     IGDKYLGKKRYYGYWRDSLAEIEGKAVNALQAIFLADWNFVTGKNLMQEDLLHKYLPV
                     ISPDKQRFLPMQIVADGPDSDYDSIMQLYFAAITYAKHSIRISTPYLILNESLLSALK
                     TAAISSVKVQILVPDKPDHFLVYWASRSYFQQLLDVGVEIWTYHKGFNHSKILIVDDE
                     FLLIGTANMDLRSFKQNFELAAAIYDKIVCAEAIKQFDEDLLSSGKIIPEKFRQRSML
                     QKTKESICRLVSPLL"
     gene            45663..48485
                     /locus_tag="PLW08_00600"
     CDS             45663..48485
                     /locus_tag="PLW08_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPL51138.1"
                     /translation="MKFINYPLSENLDTIVQELAKPDSILIFPTLSAAKRASREYLIN
                     WDLSWRGFYSMEELRSALILPKQPLLTDEKRLLCLFMVMEESERESFHIYNYGDIVDW
                     GKRFFDFFEELCEECIALSDLEEMANSGAFPMQEWQEIYLQKILNIRSNYQKYINALG
                     FSDTIFYLNADNISVPWQNCDIYYINQYYYSSLEKQQIKALEDSGNRVTIITHGLETE
                     SNGENWKIKDFNLQESWNSLRKKPSIEIIETDNETQMALSFLSWNLEQDCHNCAIIDS
                     TFHLKSYSHYFPEEHFAKPESYSFCEGTIYKMLSAIATGLKATKDTNGYLPIKILANY
                     ISEDWFCRYFYAEQGNLKLDDYISQLRLELGFLINWDYLYVDYALFLTADLPTLKSLV
                     WEYYNLINAFGKIQNLKELCNLIDSPNCLQLNKLLEDNEKLYTDILPVFWERMANFMA
                     IENLGLVNSWKQIFAEDSIGLNLLDLLLNFLTTGKISYQRNEALTPEWEISNLLDARN
                     RSFSTVAFFQMIEGIIPSSPTPVWLFNESQRAKLGLKTFNDIRNWERYYFCRLLLCSE
                     RAICFSYINQERDISPSSFLGELEELLKNEELDLFKKRKILVSLPDVYNWELVNKTIP
                     ELEDQEICNINKDLTEDFFIIPSDPIADFPMNNQINFSASALIQFLKNPFLWFVENKS
                     KLSIQNWEAEETISYKLFGNIMHTYFSTTLGELKGIHESHTILETLFGNSKKLEEQLK
                     NVIASEKMIYQIPKNYNAVFLGEILAKKLSESLFFFYQDWLKKQVEGRKFILIPEEDK
                     ISPAEMKYKQLGKVNWGNKEYIIGIRGKADLRIELENKALIVDFKTGSHDYRQLCIYE
                     WYYYLLDDVSAPNDVSSIFWNILDPTDKVEGVNEEKRNKLKQQILDNLEFCLANGYST
                     ITKATDRQRLQNITRADLISRKGGLK"
     gene            48482..>51039
                     /locus_tag="PLW08_00605"
     CDS             48482..>51039
                     /locus_tag="PLW08_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPL51139.1"
                     /translation="MTENNCRIICASAGTGKTYRLSVEYLSLILKYYGKNEFSLDNIL
                     VLTFTRKATAEIRDRIIKHLELLVSEPETEEEQKNQQELLLNLRKLNKTKEITLSEQE
                     RNLLLSARKELICDQSHLQVMTIDSYTGQIFRNIVRPLKNIEHYEIDTEAVKKIMPFL
                     LDHLMEPDLREKIKNLLSRKVSRSLDDYTKFFCSLIEQRWLFYMITKRQAVHNSSLNG
                     TLMNLNPETEPAEFYTQFLKEMQRIIALVANICSDLGKASLEDYFNKDFRNLAVKGAL
                     TPVAIFNNLKQLNSDEAEKLLYILDKGTIWNKQKISNKKFEAENIELENAQNEAKHHL
                     ADYLIWKLYLPEQREIEELWSSVLERYDNLIYRYKNLTYDDITWFTFETLFSSEPPFL
                     NPEDAVSATEFYQFLSHRTRFLLIDEFQDTSLIQFNILKPIIEEITAGEGSKPFGGLI
                     VVGDEKQSIFGWRGGERDLLLNLKDIFPALGDLTVEALDKSYRCGPTLMEFINQVFMN
                     AELCSLLQEKEMQWDYTHIESVEITKEPGTEIEFCLKNFSTSVANADNEKDIYTDFVE
                     RMVLPALEKDDSGSIAILCRTNEELTKIQQALDISGRASLFQPNRSIIEHHYVAPLLS
                     WLRFLAWGDWMDFISFLRSDYVLINTKVLKQTLNIISRTLEIQNERQKPVRPDFSTIP
                     VVNEFYQLSWEQENKKATEICREIIDLCLPSKQLSERDYLNLHRFLDILSTRQLTDSD
                     KGISFPDLLTYLKDNANAEDFKQVSINADDSLQLLTIHKSKGLQFKRVFVFCNLSGKH
                     STDSNTLNWALEYEGNTFNRIRDYGISLHYQKILKSSSYANLYLQNQKRKQLEE"
     assembly_gap    51040..51139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51140..51640
                     /locus_tag="PLW08_00610"
     CDS             <51140..51640
                     /locus_tag="PLW08_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51140.1"
                     /translation="RHHRPVNWNKLVPVELPAVKDLKTYYLTERPNLIGDLLHFYMSF
                     IIRNTPKEHNYAYYRCLNRYGAIFTVFQIEQLADRCQKVCDSNPYLFTSGWDKIFTEQ
                     EILGDSKIMRIDRLMVNTAEKVVLIADYKSGDIYDSNQLTNYKTALSKLSVLKGYNID
                     TRIVIV"
     gene            51756..52007
                     /gene="purS"
                     /locus_tag="PLW08_00615"
     CDS             51756..52007
                     /gene="purS"
                     /locus_tag="PLW08_00615"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPL51141.1"
                     /translation="MLKAKIYVQLKANVLDPQGKAVTNSLHNLGYTDVIETRISKYIE
                     MSFRNDNREKVSQEVEKICQDLLANPNTETYQYEIISEE"
     gene            52012..52698
                     /gene="purQ"
                     /locus_tag="PLW08_00620"
     CDS             52012..52698
                     /gene="purQ"
                     /locus_tag="PLW08_00620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPL51142.1"
                     /translation="MRISVVTFPGSNCDYDIYRVCKSRGHQTKLIWHKDSDLEKPDLV
                     ILPGGFSYGDYLRCGALARFSPIVHEVIAFARKGGLLVGICNGFQILTECGLLPGTLM
                     MNENLHFICQHQYIRVETEASPFTQGIGKGKVMDIPIAHKEGNYYIDPEGLKQLIAND
                     QILFRYCDKQGNINIASNPNGSLDNIAGICNKQRNILGMMPHPERSATETVPTKDGRW
                     IFAAIENALE"
     gene            52670..53377
                     /locus_tag="PLW08_00625"
     CDS             52670..53377
                     /locus_tag="PLW08_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPL51143.1"
                     /translation="MLPLKMLWNKILELLIPPACLVCGHRIDDRYQVICPDCEAKLYL
                     IGEGTCPVCGSENKELPCEVCAEGNFAFDSAISVFRYSGPVRDLIHILKYNGYSSPAG
                     YFALPIAELIESKPVLKNYDYICAVPLHRVRKRERGYNQSDLIAYSVAKLIDMPYFNP
                     VKRRINTLSQTLLSRDHRIKNLAGAFQVKDKNLVAGKKIILIDDVFTTGSTLNEIAKT
                     LRSAGADKICAITVARA"
     gene            53379..53777
                     /locus_tag="PLW08_00630"
     CDS             53379..53777
                     /locus_tag="PLW08_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51144.1"
                     /translation="MNKEFWEIRPETSESEINEMIEKVAKFIAERHLAPAGILLFESF
                     RPLHGIGSQALFFILPVAEIIFDSKKYQQFAIMMEDENNIKKLINRMDELDEIYTRER
                     RQENKLKKQHRKAKRKAFYHKLFKKINKTA"
     gene            53793..55655
                     /locus_tag="PLW08_00635"
     CDS             53793..55655
                     /locus_tag="PLW08_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPL51145.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWIDGEFRQTIRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYHNPNIKFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMVTGVVASMTGESAERAALGAGAIVMDLIASNDKRMNHEKIERIRELRPDM
                     ILMAGGEDGGTVKHVVEMAELVASADPKPRLGSGYKLPVIYAGNKDAREEIIKTLGEK
                     VDLIITDNIRPKLEIENLLPAREKIHNLFMEHVMKQAPGYNKLMEWTVGPEHEQVPIM
                     PTPAAVGNIMQAIAKEENIEVVGVDIGGATTDIFSVFTKDFIFNRTVSANLGLSYSIS
                     NVLASAGLENIMRWVPFDINESELRNMIKNKMIRPTTIPSLLEELVLEQAIAKEALRL
                     AFEQHKSFASSLKGMQKQRDISEAFSQSVSGETIVNMMTLDLLVGSGGVLSHAPRRNQ
                     TVMMLIDAFLPEGITRLSVDSIFMMPHLGVLSEISTKAATEVFRKDCMIYLGTCVAPV
                     GKGKIGKPALYAKLELPDGSVFEEDIPFGEMRLIPCEIGQVAKATLKTHSGLILDADR
                     KKELTVDLHGGKVGIVLDTRGRKPFVLPADKNERISALKKWMKVLNAYPEHILS"
     gene            55668..56798
                     /locus_tag="PLW08_00640"
     CDS             55668..56798
                     /locus_tag="PLW08_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51146.1"
                     /translation="MGQAYSAGLTVTDNTILRKERILPLKGQVLVKKGDKVKAEDVVA
                     ETLLPGKVIPFNLANKLGVTPQQLVNYIKIKPGDKITQKTVLAETKGLFGLGIMKTEI
                     LSPIEGEVENISSVTGQILLREPRIPVQVKAFIDGLVTDVIEEEGVIIENKSAYIQGI
                     FGLGGETFGELKMIAESPDDELDVAKIDASCAGKIIVAGAFAPYHAIDIARKNGVKAI
                     ITGGIDDQDIKKLLGYDIGVAITGHENIGITIVCTEGFGRINMAQKTFNLLKHFEGYK
                     TSIHGRTQIRAGVIRPEIIIPLEFEEQELVAKEVSMPILEIGTVIRIIRQPHFGHIAK
                     VTALPEELTKVESETLVRVLEAEFENGTKIIVPRANVEVIES"
BASE COUNT        18248 a        10103 c        10913 g        17349 t
ORIGIN      
        1 atttaaggca agaaccggac gcacactatc tttttttaaa ctaagttcct gttcggaatg
       61 attaaaagga aataagggaa ttaccgcatt tgctgtataa agagaactaa cccttttttt
      121 agcttcagca aataaggcac cttctaaatt caatcgttcc gaaacggaac taacataaag
      181 agttagatgc caaaaagagc catctttggt aaaacttata gcatcatcaa aatcctttgt
      241 gctttcatca tcaatggaaa aagcaattgc ttcaggttgt gttgctttcc aaggcagaag
      301 tttttcctca atcaataaag aagaaaaacc aatgggaatt ccagcattgg caataacagg
      361 atcaatatat tcaggtgttt caccgcaaaa tttacgaaat tcaagaattg cctcttcggg
      421 tttaagcaat ggatcttggg aaagaataag ctcttttaat tttttgtatt ttttagccgt
      481 ctgcaattcg ggcaaagcat tataaagctt atgctgactt tctctgctta attctgcacc
      541 ggaaaaaaag gcattaacat cctgtaaaaa ttgcctttct tcttcttgtt gttgaagttg
      601 gagttgatat tgagtaattt cctccggtgt tttcagacga tatttatttt ttttacagtg
      661 tatcttttcg gggtgattat gtaaatattt aaaaagtgca aatatctgca catctgtttg
      721 caaatttagt tcagttgcaa tttcctgaag agtgcattct ttctctttta gaaagcggaa
      781 atcggattcc tgaaattgct cctcaaataa attaacctgg tttataaaat ttggcaatgc
      841 ctgatacggt tcactctcca agtatttttc tttgctttgc agaataatac gggattcggg
      901 taaaactaca taagttcctg tttcagtaat aacctcaaga cgattatttt tttgagcaat
      961 tacatagcca aaattaagaa tccccttctc aaaataggca acataaacat ttatgggtat
     1021 attcaccaat ccttcttaat tacatatttt ttggcgcccc agagatgact tgaacatccg
     1081 accaacggtt taggaaaccg ctgctctatc cactgagcta ctggggcgca tttttattta
     1141 ccaatctgaa aagagagctt ttttgtcaag tttacattga caagctatgc ttgccggaaa
     1201 agtgtgaaaa ataattggca gcttttatgt tatgccaaat tgataaagga aaacaggtaa
     1261 aaatgaaagt agctattgtt ggtgccacag gtgaagttgg caggatgatg atcacctgcc
     1321 tggaagaaag taatttggaa atttccgaat tggatttgtt tgcttcctca aggtctgcag
     1381 gcacagttct ctattataca gatcaaccga ttaaggttca ggaattgaaa caggattctc
     1441 ttttaaaaca ttacgattat gttttgtttt ctgctggagc tggagtagcc aaaacttttg
     1501 ctccaattgc tattcaaaca tctgaagtag taattgataa ctcttctgga tttagaaaag
     1561 aacccaatat tccacttgta gttccggaag tgaatggtta cttgttaacg ggttacagtg
     1621 gcatagttgc caatcctaat tgttctacca ttcagattat tttgccttta gcagtattgg
     1681 ataaactctt caacctcaaa aaactgattg tttccaccta tcaatccgtt tccggaagcg
     1741 gtcatcaggg aattgtaact ttattaaatc aacgcaaggg gaaaaaagat aaaggtattt
     1801 atccgcatat aattgatttg aatgttattc ctcagattgg aggttttttg gatagcggat
     1861 attgtcagga agaagaaaaa atgcactttg aatgtcgtaa gatacttaat aagtgggatt
     1921 tacttgttag tgccacaact gttcgcgttc ctgttattta tggtcacagc gtttctgttt
     1981 atgcagaatt tgaaaatgag gtagatatac ctaaggcaga aaaaacatta caaaattcac
     2041 ctcgtattgt ctactatccc aatacttata taactccgct agatttaggt tcttccaatg
     2101 agacccatat ctgcagattg cgtttgggaa ctgatgaaaa gtccctaacc ttttggaatg
     2161 taggacataa tgtgcgttta ggagctgcag ccaatgcagt taatatttta attacccatt
     2221 ccaaatattc aggtcggctc taatgattga tattattgaa ctgcgtcata gtttacatcg
     2281 tattccggaa cttgcttttc aggaatttaa gaccaaagct atgctggaag aaaacatttt
     2341 gcgtctttta caaaaccagc cggaaaattt gtggcatctt cattattttc aaaacagtcc
     2401 cggtttgcta ttggaatatt ctcctgcaaa gggaaattat ctgttattta gagctgatat
     2461 ggatggctta cctataacag aaaaaacagg agttcctttt gcttcggaac atcctaattt
     2521 gatgcatagt tgtggacatg atgtacatct aagtatttta atggggttga ttcaatatgt
     2581 agcaaatacc ttaccccaac gaaacatttt gtttcttttt cagccagccg aagaaggtga
     2641 aggaggagca aaaagtattc ttgctgaagg cattattcaa aagtttgaaa ttacttctgt
     2701 ttttgctttg catattgcca gtgaacttcc tgtaggttct gtttctgcta aagaaggaat
     2761 atttttttct atttcccagg aatttgatgt tcttttttat ggcaaagcca gccatattgc
     2821 ctttcctgaa aaaggaattg acgctctctc tgcaggaatt aagtttatga atactattga
     2881 ggataaattg gcagaactga aaaaaacgga aaaaatcatc ttccacatcg gaaaaatgag
     2941 tgccggaact attcgtaatg ttattgctga taaatgtgtt ttggaaggaa ctcaccgttc
     3001 tctttccccc aaaatcagcc aaaaaataaa tatccttatt agagatacag ctgagctaat
     3061 tgccaggaat accaataccc gaagtgaagt taaatttctt gcttcctacg atcccgtaat
     3121 caataatcct gatttagtta atcgcttgca ggatatttgc cgtcaacaaa atattgaata
     3181 catacctgct cagacctcta tgacaggtga ggactttgga tttttcacta ctttatatcc
     3241 aggattgctt ttttggttgg gtagcggatg ttcctatcct ttgcattcgg ataaattttt
     3301 accccaggat gaatgcctgg aaatcggtgt aaaaataatg tccgctttcc tgacaacaga
     3361 atagtcccgg atttttttac aaagagaaaa agaggataaa tggataattg tgaatgcaaa
     3421 atgtagaatg aaaaattgaa tttaacctaa aaaatgcagc tgcaggaaaa tgggttgtca
     3481 aattctttct ttgctaaaaa attcgtagaa ctacgaaaaa taactgtcct caatttatag
     3541 ctaatctgaa ttcagcaaac aaaatgctaa atatatcttt tcttcccagg gttaaaaccc
     3601 ttctctaata tctgtcgttc ctacggaact ttttctcgga aaaagagata atgacttttc
     3661 tgagttcttt tcaccgggtt aaaacccgtt gttaatatct gtcgttccta cggaactttt
     3721 tttcccaaaa taaatatagt tttttaggtt cttttcaccg ggttaaaaac ccgttgttaa
     3781 tatctgtcat tcctacggaa ctgatactcc aaatcatata aatctctttt atcctgttca
     3841 tcacatacat attttctctc tgccctctgc ctaaatcatc ctgttcatcc tatacttatt
     3901 ctcattttta atctgcgtca tctgcgaaat ctgcgtgagc cctttttttc attctcctct
     3961 tactcgccgg gttaaaaccc gttgttaata tctgtcattc ctacggaact gatactccaa
     4021 atcatataaa tctcttttat cctgttcatc ccatacatat tttctctctg ccctctgctc
     4081 aaatcatcct gttcatccca tacctatttt ctctctgccc taagctctct gccttctaaa
     4141 ccctctcaac cttctaaact ttatttcacc agcagcattt tttgggttgc ctgatagttt
     4201 ccactttgca gacggtagaa atatacgccg gaggcaacat tacgtccctt ttcatctttt
     4261 ccattccaga tcacattatg cattcctgaa ggaagttctt cattcactaa gcgttttatc
     4321 tgctgtcctt taaggttata aattgttaaa gttgtttttc ccggtttagc caaagtaaaa
     4381 gatattgttg tagtaggatt aaaaggattg gggtagttat tattcaattt agttactaag
     4441 gcaggaattt cgtaatcaga gttcggtata aaaggaaagg gaatattaag tatctggctt
     4501 ggagttccat cagtgtttaa atatttaact acaatataat atttataatg tcctggtaga
     4561 gtgagcactt cgtgatagga tatctctgga gaatcctgaa ccaaataaaa ctggtctgta
     4621 ttgaatttcc gatatacctt aaaacccaca ataggcaaaa cagtatcttc cggtggctgc
     4681 caaaagacat acaaatcccc tgtatcatta ttaccaatat aactaatact ttgtggttga
     4741 ggcaaatcaa ttaaggtaaa ttcagtataa tgaacaggat tactcggatt gataataatg
     4801 gaagttaagg ttgatgattg atgatatggc atagtagcgg ttacagtatg aatcccgtta
     4861 ggcaggaaaa taataaaatt accctcggaa tcaggatgac tggtaaaacg ctgattagac
     4921 ctgagagttg ccattgaagg atcaagaccc gaacttggat aaacaactcc atacaaatat
     4981 ccggttttca aatttacccc actaacggta aaatcatcaa taaaccatcc cggaccggat
     5041 atttctccat ccgagccaaa acggaaacga aacataaccg actgtcctgc atattgcgat
     5101 agatcaaaaa tggctaattg ccagccatct gaatttcctg tccagcccgg atctccattc
     5161 aaaccgttta gggaatatcc attgtaaccg ctaacaggat aaattaaatt ccaaacaact
     5221 ccattatttg tagaaataga aacattaccg ccatcatttc catattcaca gctataataa
     5281 tgcctaaatt gaagctgact gtttgtttcc aataaatact tgggagtata gagattataa
     5341 tttacataag aaggatagtt tccacttaag ttagttgccc aaaccttggc tccggagaaa
     5401 ggtgttacct gagaaggatt tccccaaacc caaccggttt cggaaacgaa ataaccatta
     5461 tttaattcaa aatcctgaaa gacaacagga ttattgtaag ggatactaaa tgatgcattt
     5521 gttgaactac cattggaagc agtagcagta aacaataagg gaatttgagt aacagaaaca
     5581 tcaagcatag aggtattgat caaaaatttt atttgataaa tatcgttagg accaatttta
     5641 tcaaattgga gaagcggttc ttcaataatc agctgctgca atggactgct taaagttgct
     5701 tgaatactgg tcgcgtcaac agcggcaaaa ttttccaaat taacaatcag attaaagaat
     5761 tcaccactgc taaccatacc gtcaaaatca tttagatgat catcaacaaa aaatgaatga
     5821 tagagcaaac aagatgcaga tacctgcaaa ctgaaattcc ttgtccactg caattccccg
     5881 gaagtaatca acagttcaaa atacagcact tccccatcag gacagttatt cgcaatcgta
     5941 aaagtaaagg gttctttgtt cacaccgttc attccaggtt caatcggata atattcagca
     6001 gtggcattat tgattgttac atattcactt gcagtgctaa tctgtccaat tacattttcc
     6061 gctgtcagat tgcctgtatt ctgaatttta gcccctaatt ccactatttc acctggctcg
     6121 catattccat tcatatttgt atcatttaca taagttgctt ccagaagcag atgagccgaa
     6181 gaatgatata tgggaacatt agtaataaat aatgccttac cgttacttaa gggggctgcg
     6241 gctgtaggat aggtattatt gaaagtatat tccaagccca cttttcccgt atgatcttca
     6301 ataccgatag tgcaatagtt gccatgtttg tttccggatt gagaatcaat attattaaat
     6361 gtctgatact gaaatttaat aggtccgtct ccaaaacttg taggataagc tgcctggtca
     6421 tataaaatac actggaaagt ttctaaagcg gttcccccat tgccattttt cagtttatac
     6481 cattctatta taaaggaatg atgatttcgg tcaaaccagg tataaattcc actccctgtt
     6541 ccggttgcca aatcatccca gaagggagca atcataggat tgggacccat aggtccggga
     6601 agacggtaat ttctaaattc cgcattggca gtaaccccca tcgcaataaa accattagaa
     6661 caaacggtta tttgattgta gaggatgccg taaaactgaa aagggaaagg taaggacaca
     6721 acttccaagg catccgaacc aacttggtct ccttcattgc cacttgcata accgtctgaa
     6781 attgctaagg tagttcccaa accaccttcc aaaggcgaaa tctcaatcca gtcataaact
     6841 gcggcttcgg cataattggt atctgaccaa tcataaatta cataaccgta agtatcagga
     6901 ccaagaggat cctgttgagt taccacaccc acggtaaaag taaaatccac aaattgctca
     6961 aaaccatcac tattataaag ctttaatcgc ataggaatta gcattccggg caaaacttca
     7021 gttctagcag aaagggtaaa acgattagtt ccacaggtta ccagaattcc tgttggcaaa
     7081 gagccgaatt cagcatttgg atcacttacg ccaactaagt cgttctgagt atataaaatg
     7141 cccaaaacat tgtctgcttc aacagttcct gtatttttaa ccgtaatact gaattctgcc
     7201 acttctcccg gatcaagatg ctgattatta ctatccatta ccaagtagga aacaaagtca
     7261 atattaggtg cttccacagg aataaattct gaaatatggt aggaaacgga attagaatcc
     7321 gtaagattaa gatataacct taacattgtt tggtgaggag tatctggagc aatttgaatt
     7381 atgataggaa ctgaattatt tcctgtttct ccacctaaaa tgtcaggata gataactgaa
     7441 gaattcgcta tagtaatata tgggctgttt gaaatcacac tgccgctaat tccggtaatg
     7501 atatctgttc ccgtattggt taagcccaaa aaaagttcaa ttgtttcccc gctactagca
     7561 agtccatttt cattgccttg cgaaggtgct atattatcat catcaatcac catcgttcca
     7621 ggaaccagag ttgccatatt tacaacagaa attgttgtct gaataggttt aaaattgtgt
     7681 ttgcttaccg ttaaagttgc atttccggca agcatatcgg aaggaagcac taaaatagct
     7741 gttccatctg ctcctgtgta attacgagct aaaataagcc ctcccatatt taaaactaca
     7801 gcagcacctt ctacaggatt tcccgaatta tcctgaacag caacatcata tagttttaat
     7861 cccaaaggaa ttgaaatatc agaagtaaca tcaaagtgat tgggaatgcc tgtaaagacc
     7921 tccatcgtgg gatcacccat caaattacac caagcagcaa aattagttgc attcgttgga
     7981 gaagaaaccc caaagagttg atgtagatag aggcgtccat ttaataatgc ttcacccata
     8041 gttcgcatat cagaagcaaa tattccggca aaaattcctc catggatagc gttattaaaa
     8101 gtagtatggg tgctggaagt acacatccct attccggtta cggcaccttt aggagctgct
     8161 gttgttccat agcggatcat tccttccact tctcctgcac cactgtaatt attagttcca
     8221 caggttatat ttacggtatg aaacagtttg tagccattaa aaagagctga ttctgcagga
     8281 ggagaccaat caatataacc cctgaaactg taaaaaccaa ctccctggtt aagtgcctga
     8341 ttaataattg tgtagttggg agaagcacca tagtcctcag tgaaagtata atcgggatta
     8401 gttagtaggc tcatttcttt gatatattta tgaatataca ttgtggaaat tccggaagga
     8461 gcattatctc ccgaaagcat cattttattc agccagctgg cagtagctaa attaatatcg
     8521 cgttcataaa gataaatttt gttcagaact accaaaaatt gcgataagtt ctcaacagaa
     8581 atacgcccga taaaacaatc gcccagttga tcgtttcccg ctaaaaaagt aaaattataa
     8641 tcagttactc cgctattagc agcagaataa gcagggatag gataacttcc tgatgtatca
     8701 ccaattaaaa caacaaaatc gggacgagtt tcgggattat tatatttagt ttgaatgtaa
     8761 tttttaattg aggtagttgt atttcccgct tcattagcag ccgtgctggc aaccattaca
     8821 tctgcgcctt tctgtttttt ccagagtaca tattcattca aagcactggt aaaattagta
     8881 tcactgttgg tgccataaat tattaaataa cggggaggag tattggttac tacggcatca
     8941 cgataatcac cataattcaa gatcattgaa tcatatattt tatcaaagct ggcagaaata
     9001 ttagcaggtt catttggcaa ttcatttata ccctgttcct gagtaaaatt taggcggaat
     9061 tcaatattat gatttacagt cagttcaccc gtttgagcat tgtaagaaaa gggatttatc
     9121 tggatagtaa taatccgaaa gtcccttaaa atacttggtt cactatattc aatcagcatt
     9181 tccggatagt tattaccgct attgtaatat tcattgttga taataaatcc cttggggctt
     9241 tccgaactat taccttgttg cacaggatag ggaagaaaat tgggaataac tgtctgttgg
     9301 gtagaaagca cttcaatatt tacgcctccg gtatgaggaa tggctataga tgtacaaacc
     9361 acaggcaatt caggcatacc tgtttccata agagttccgg aaagaggtag tattatttta
     9421 tgataaactg ctcctccgta agtttcttcc tgcatagaat attctggcag agtaaaattt
     9481 acttgcagag aggaactatt tttagctccc aaagtaaaag ctgaatttag attagccggc
     9541 agattatcat taatattagc aaaaacgatg caacccgcta ataatatgag tattaaggca
     9601 atacttctct tcattggact tccttataat gtgtaggatg gttttctata tattagagca
     9661 aatattattc caaagaaaat caaagattat agtaaagcag catttcgtga gggtgcggac
     9721 gagcagaaac tgcttcattt tctttgagtt ttaactctat atgactttct atgagttcct
     9781 cgttaaaaac atcaccttcc agtaaaaatt gatgatcttc tttcagagct tgcattgctt
     9841 ctttaagatc agttggaata gagagcaact gtgctttttt atcttcactc caggcaaaaa
     9901 cattatcatc aaaaggaccc agattatatt tggctggatc aatctgtttt ttaattccat
     9961 ccaagccagc caataaaatt gcgctcattg ccaaataagg attgcaggtt ccatcaccgg
    10021 ttctaaattc aaagcgtttt gtttcaggag tattagcata tttgggaatt cggatagcgg
    10081 cactacgatt ggcaagacca taaaaaagtt ttacgggtgc ttcaaaacct ggaagcaggc
    10141 gtttaaaact attggttgaa ggattagtga aagcaactaa agcacggccg tgaattaaaa
    10201 ttccccctat aaaccaaatt gcttcttcgg agagatctgc ataaccaccc tttttataaa
    10261 acaaattttt cccgttttta tgcaacataa tatgaaaatg cattccattt cctgcatgtc
    10321 caaaaaccgg tttgggcata aatgtagctg taagtccata ttgcaaagca gttttacgaa
    10381 tgatatcctt cataatcatt acatcatcac aaattttagg aaaggcaagc agttctgttt
    10441 caatttcttc ctgagcggaa ataccaactt catgatgatg atagcgaact ttaatatcct
    10501 gttcttcaat caattccaca attttttgcc gaatttcgta aaattgatca aaaggtgtat
    10561 ctatatgata ccctttaaca ccttgtaaac ttaaggaacc agggtcttca ttatcatccg
    10621 gaagcgcttc tttacattca ctggaggtaa tattataacc agcagaaaag gaatcggaac
    10681 aatactgaac tgaatcaaaa agatgaaatt ccagttccgg aatccaggta gacatatctg
    10741 caattcctgt tgaaagtaaa tagttatgcg ctctaagagc aacactgcga gggtcttttt
    10801 ttacccctaa acgggtttct gcatcgcaaa tggaacaaat cattctcagg gtgggggtct
    10861 cataaaagga ctccagatga gctgttttgg aatctggcat taaaaccata tctccacttt
    10921 ccacacttct cattccagga atggatgaac catcaaaagg tattccaact tccataactt
    10981 cagcaatatt acgagccgga aatgtaatat gataccattt accatccaga ccgcaatatt
    11041 tcaggtctac ggcttttact tcatttgcca gaataaggtg ttgtaattcc ttcaattcca
    11101 ttttgttctc cttattattt taattttata tttttaattt atgtaaagtc aggagtcgtc
    11161 actgccgaaa caaattttac tcttaccaca aactcatttg gcagttaagg actcgtaacg
    11221 acttaaaaag ataaaacaca gataactaaa tcaactaata tcattttttg aaagataagc
    11281 cacttatcat tcctgcgaaa acaggaatcc ctatatgata aaatatattt ctgcattttc
    11341 aatctgaaat atatataagc tcgtcataaa tcatagatac cataaaagtt attgcacaat
    11401 cagaagttat caaggtgtaa ataactccta acttaccttt ccctaaatta taatctttta
    11461 tctgcgtaat ccgcatagtc agtgagagtc aacttttata tccgtacccc cataatttgc
    11521 attacaaaat ttaccggcgg aataataatt ttccctataa tattccaacc caacaaagtt
    11581 gtaatgataa taaacgcaaa caaatacatc attcctttgc gttcttgcat tgtccattta
    11641 taatatgcct gatcggttaa aaacattcct ataactttgg aaccgtctaa agggggcacc
    11701 ggaatcaggt taaaaaaggc aagcaagaga ttgagataga tcacaaaaaa acaaatatat
    11761 ttaattacaa ccgaaaaacc gcacaaatga aagatcaagg caaagaaaat tgccaccaga
    11821 aaattggata gaggtcccgc taatcctgtt aagccagaat ccctttttaa gttcttaaaa
    11881 ttatagggat tgaaaggcac cggcttggca taaccatata taaaaccgca ggttaaatat
    11941 aaaaacaacg gcagaattac agttccaaac cagtcaatat gttttaatgg attcagagtt
    12001 aagcgtccgg ctcttttagc agaatcatca cccagccagt atgccattaa agagtggctg
    12061 aattcatgaa taataatgct gtaaaaaacc agaaaaatta taacagcatt taccagcagt
    12121 tttataatat tgggactgat catatcttct ttatctttat gtaattacgc tttcccgcac
    12181 gaataatatc tccatcttta agattaacta cagcatcaaa agcagtaatt ttctctccgt
    12241 ttagggagac agctccgcct tgaatgagac gttttgcttc accgtttgta gagcatattc
    12301 cgctatccac caataaatta gacagtttac agctgttttt gcttaccaca aattcaggaa
    12361 tatcattcgg cacttctctc agggaaaatt gtttttcaaa ggcatattcc gcttcttttg
    12421 cttcttctgc gctatgatat aaagtaacaa tttccctggc taagcgtttt ttaattagca
    12481 tcgggttagt tccggattcc agttcttttt ttattttttc aatttcttct tccggcatag
    12541 tggtggcata atagaaatag ttcaaaataa tattatccgg aatggacatc actttaccgt
    12601 atttttctgt gggtggatca aatacggcaa tatagttatt aagagatttg cccattttat
    12661 ttacgccatc cgtgccaatc aaaatggggg ataaaatagc cacttgttct tccatcccaa
    12721 aatgcctctg catatctcta ccacaaagaa tattaaattt ttgttctgtt gctcccaatt
    12781 ctacatcgct attaacagca acggaatcat aaccctgcaa aacaggatac aaaatttcat
    12841 gcattcctaa agctaaaccc tgttcatagc gctgacgaaa ggtttgatgt gccataaatt
    12901 gagctaaagt aaatttccct aatagcttaa ttacatcact cattcccatt ttgccaaacc
    12961 attcactttg gtagcgaact tccgtttgtt ccttcttcag aacggtatat agttgttcca
    13021 taaatttttc actgtttctt tttacttgtt cagcagataa agggggacgg ctttcatcac
    13081 gacctgtagg atcacctatt tgagctgtaa aatcaccaat tataatcact ccgatatgac
    13141 ctaaatcctg aaattgccgc attttccgaa taggaaccaa atgtcccaaa tgcacatcat
    13201 aacccgtagg atcaatacca tattttattc tcagcggttt tccggttttt tccgctttgg
    13261 caaatttggc tttcagttct tccaaagtga ttatttcttc cacacctctg ctgattaatt
    13321 tcagttcttg ttcaaatgcc atttaaccat ccttttttca ataaagtaat taaaaaggac
    13381 aagaaagaaa aagcggatat ttgtcaaggt taaaagggta aacaagtgaa agggttaaca
    13441 aattaacaga tgaattgaaa aggtggaaaa gtggaaaagt gaaacttaaa atgaacttta
    13501 taaataactg aaaattagtt gagaaggttt agaccaccgg aaggttgaaa tcctcgtgta
    13561 ccaaaatcat cggaaggttg aaatcctcgt taacatccat aaaatatgaa cttatcaatc
    13621 taatcttttt ttcttcccta aaaattctct aaccacaact aaataaatat aattccgttt
    13681 tcttccttaa tagcattgct tacccattgc ttacccattg ctaatggcat tagtaatggg
    13741 taagcaatgc tgcaacaatg caagatggaa aaagctgcta taccctgaaa atagtggact
    13801 ttatttaatc ccaataatgc agttgaaaaa aaatggcagg gaaaaaaagg aaaagaagtc
    13861 ggggctttta ttttacgagg ggcttacgca cccatcgcta ttattgtgtc gccctcaggg
    13921 gctttttttt agaggaaaag agaaaaagga aaagaagaaa gggcttttat tttacgaggg
    13981 gcttacgcac ccatcgctat cattgtgtcg ccctttgggc tttcaaaaaa tcacaacctt
    14041 ctcaatctta tcaaccctct caaccctatc aaccttctca accttctcaa ccttatcaac
    14101 cctctcaacc ttattttacg aggggcttac acatccatca ctatggaagt ttcgcccttt
    14161 gggttttcag acctactaca ttaattggga ttagtataaa aacaatgaaa atagataaaa
    14221 taaaccctaa aaactcttta atcttgttcc tccctggctt attaaatcca tgtaactcct
    14281 tttttatcca taaaaaccgt atttttaata actgtgtaat ttttgtaatc tgtgcaaggt
    14341 aaaattgtca gtcccctaaa gagattccca atccccgggc agttgccaca agatcggaat
    14401 caaggtctat ataattatat ttttcaatag cttcagtgat gggaacagca acaatattgg
    14461 gatgacgata agccaccata gaaccccatt tttcctgtaa aaccagttca aatgccttca
    14521 ctccaaactg agaggcaagg attctatcaa aggcattggg agttccaccc cgttgtaaat
    14581 gtcctaaaat agttacccga atatcaattg tgcatccggc atttttaagt tcttgagcca
    14641 aacgcaaacc tgctcctccc agtttaattt tcatagctcc cggttcatca cattctacaa
    14701 aagtcatatc tccatctaca ggtctggctc cttcggcaat tacaatattg gcaaaatcgt
    14761 gtcctctatc aatccgttca ttgatggctt gcataatgtt tttgatatta tacggaattt
    14821 ccggaatgag gcaaacctct gccccacctg caattgcaga atgaagcgct atccatccgg
    14881 caaaacgacc cataacttct aaaattaaaa tacgatgatg actggctgca gtagtaacaa
    14941 gtttatctac tgcatcggta gcaatatcca ctgcagtttg aaagccaaaa gtaaaatctg
    15001 ttgcagaaag atcattatct attgttttgg gaacgccaat aaccggacaa cccagctcaa
    15061 aaagttgctg tgaaatgcgt tgagaaccgt cccctccaac attaattaat gcctcaatat
    15121 tttgataacg gaggcggtct aaaaaatcct gggagcgatc tacagtagtc catgttccat
    15181 ccggattttt aatgggccag ttgaaaggac cccctttatt ggtagttccg ataatagttc
    15241 ccccctgaat atgaattcct gcaacctttt cgggagtgag ttctataata tgcatagggt
    15301 cttttaaaat cccattaaaa gcatcaatgc tgcctaaaac ctcccagtta tgttcaaaag
    15361 aagctctttt tacgatagct ctaataactg cattgagacc agggcaatca cctccgccgg
    15421 tagcaataag taatcttttt ttcataattc cacctttttt aggttaataa ataaagcccc
    15481 cgaattttgg ctgattaaat atctctctta ataagaacac gataaccgga aagttcttta
    15541 aagccaaatt tttcatatag atgccaagca gcaggattat ctacattcac ttccaatttc
    15601 agttgataac catattcctt tgccagcgaa atacattctt ccataagttt ggttccgata
    15661 ccttgatttt gtttatttgg aacaactgcc atatgatgga tataaagtcg tcggaaatca
    15721 ttagttaccc atacaacgcc aactactttt tcattttcct ctgcaataat caatattccc
    15781 gtttgttgca gattttcttg cagggattca aaggaatcct tgcgttccgg tttattgatt
    15841 cctgtttttt cccaaagagt agatacctct tcataaattt ttttgtttag ctctttgatg
    15901 cgcttaatag aaagcatttt aaccgaattc ctgcattctg aagatatcgg gatgacgtgt
    15961 aaaacgaaat acggtatccc agacaacgac aaataataca cttggatcat tatccaccat
    16021 cattaaataa tatccttgag tgtgttcaat aaatttgagt tcctgagtgc ggttaccggt
    16081 taaataaagt tttacggtgc aatagggatt tttaaatttt tctgcatatt ccgcttgggc
    16141 attatctata aagatatagg tattcaaact ttccaggata ttcagaattt ttagcattgc
    16201 cctgtttgcc ggatggattt taaattcatt gtgggcatcc tgaaaagtcc attgaagacc
    16261 atttctggta agggtatatt tatttttggg atgagtaact tcaattttta gcaattcttc
    16321 ttctttatga ctaacgatat gaggagaacg ccagttggaa aggtctgtat tataattatt
    16381 agtaacttta gctttcaatt gatagatttt atcggaaccg gcataacgaa aataatcata
    16441 aggattatcc aaattgctga agagagtatg aatgcttttt ttaccgtcac ttacaattat
    16501 atgcaatgcc tcttcgtctt tctgatgaaa cttttcaatc gcttctttac ctgttcccat
    16561 aggagtttta gcatattttg ctgtaatcac ttcccggaac aaatcgcgaa tttttaagct
    16621 atcggcttcc cagttaacag gataaattaa tttccagata ttattttgct taaccatttt
    16681 tagagtatct tgcgcatcgt aaatctcaat tcggttgata gataaagtat ccaaatcaaa
    16741 aacccgggcg agtttttctt gaggtctgtt cgttcttaaa acgaaatata aacctattag
    16801 aataagcaaa acaagcagta atattaattt agatttcttc atttttaaac tccttggtgc
    16861 gtttacgatg taaagatttt atgcctccta cggcaataag aattaaagaa ggtaataaag
    16921 tagcaataat ttttacttcc attttgattc tgcgttcctt ttttgccgga tcaccccaaa
    16981 ggagattatt tttatgcata taataagcaa tatccaaaat gctttcctgt aaatggcggg
    17041 aacggatatt gatcatatcc tcacgcccca taagccaatc cacagcattt aagattatct
    17101 gataacgatt ttcatagatg ggcttatcgg gatcaacata aagttcccta tctccgaaaa
    17161 taacaatttt accatctttc actgcagaaa caaaatccga cctttgaaac tgcggtttct
    17221 ctttaaaata gctttccatt ctgccttcaa caattgcccc taaaggaatt ggaggattta
    17281 caaaaacatc cggatcggga ttgaaaaaca aacgggaatc caactgataa tcgggtcctt
    17341 ctaaaagagc actgcgagtg gaagtggcaa gaatggtttg aaattttaat cccggctttc
    17401 tattaaaaat aattccattt cccatataaa tgataatgtt ggatatattg cgagtgatag
    17461 gatgttgtga accgcgtaaa acaggataaa taggaaaaga gatattagta tccacaccca
    17521 tttgtctgga atcgcagaaa atatccatag ctatgtcact gcttaatttt atgccgttat
    17581 tttccagaaa atcaaaaaga ttggaatgta tttccattac tgtttcgcca tcagaaaaga
    17641 ttttatcagc cagaactacc agttttcctc cctgcattaa aaactggtct aaattgtaaa
    17701 gctgggtaat agataaagaa tcataaccgg aatgaactat cataacaggt gtttttggtg
    17761 gtggttccag cagattggta aaatgaacat tataattgga gtaaagttca ttttcatatt
    17821 tctgcctggg catcaaagta tagagagtat ccacaaaagc agtaatatcg ggcaaagtat
    17881 aacgcgtaat tttctgaatt ttcagggtca tttgatattc cagctgattt tgcattttag
    17941 gcaacacatt catagattca aaattgccct gatattcaaa aaccaatccg taaataactt
    18001 cttttgtagt ggtcttatca ttttcaaaaa tgcggaaata catagccccg actccatatt
    18061 gtagagcttt gtttttgagg tcttcaggac ttggtccgtt tataacttca taatgaaatt
    18121 tgcctttccc cgcttgttta tattcttcta aaaggtcttt aacataacgg tctaaaatca
    18181 gcatagccgc gggtaattct ttactggagt aaagcttaac taccatatta tcttgcaaat
    18241 tacttactgc atccttgctt acctgagaaa gtgaatatgc tttattctta gaaaaatccc
    18301 aacgcactct ggcataagaa ccgattagca aaaccattaa aacaatagca agcttaataa
    18361 gcaggttgga taaaatggaa ctggtctttt tttctttttt cattttattt ttcctgcatc
    18421 aggtttttgc tttggagatt aaattgagcc aacaacgcaa aaataaccgt aacagcaagg
    18481 aaaaagagaa tatcgcgagt gtctataacc cctttcagaa aactgcttaa atgataatca
    18541 aaactgaagt attgaacaat tggaaccaga ccaaaaggta atagcggtaa aagaaacttt
    18601 aataaataaa aaacagctga aataagtaaa gccaaaacaa aagccagaat ttgattgctg
    18661 ggaaaactgg aggcaaaaac tccgatggaa gcataagcta atcctgctaa aataagacct
    18721 atatagccgc aaattatggc tccgtaatca attccctctc caaaaataac aataatcccg
    18781 aagaaaacaa gagaaagaat aataatcgta ataatctgga ataaagctga taaaattttc
    18841 ccccaaacga tagatgaaag ctttatcggt aaagtggaaa gcaattctaa agttccggtt
    18901 tgcctttctt tggcaatgct tcccattgtg atagccggaa tataaaacaa aaagacaata
    18961 tggataactt caaacaaaaa acgcaattcc gctctggcta ttttaaacac catattggaa
    19021 aaaataatgc caataatcac caaaaacagg atgtagacaa tataagtgga aattgaatgc
    19081 aaagcaagtt catattcttt tttcgcaatg gtgaaaactg ttttcattgc tcttccctat
    19141 tatcttctgt ttcagcaggt tttgtttgtt cttcttccat aaattcttgt tctgcagata
    19201 acttttcttc aatttcgggc tcataatagt tttctatagt aagctcatgg aaaatagatt
    19261 ccaaactcat tttgctggtg gaaaattcta aaatcaacca gcccctatct ctgataaaac
    19321 gggaaagctc ttggcgtaaa tcaaattcgg aggtttcatc aatactattc ttatcgcttt
    19381 cattattggt gattgatgag gagatcaacc ctccggaggt ttcataaaca caaactttgc
    19441 aggtagtttc attccgggaa agaatatcca gttccacaaa aggatactct tttagaaaat
    19501 cagaaaaatc tacattttct ccctgcactt ccagaattaa catagtgctt cttttcagat
    19561 aattgggcaa gttttctata ttatcatcaa caataatttt gcctttatta attattacaa
    19621 cccgatcaca aagtgcctga acttcctgca taatatgact ggaaagtaaa accatttttt
    19681 ctttgcccaa ttcccgtata agttcacgaa tttcaattat ttgattgggg tcaagaccgg
    19741 aagtaggttc atctaaaatc aatattctgg gatcatgtaa aattgcctgt gccaaaccgg
    19801 ttctttgacg atagcccttg gaaagggttc ctattctttg atacaagaca tctttaagcc
    19861 cgcagttttt aactacaaaa ttcaggcgtt cccgaaaata atcccgtttc aatttccgta
    19921 aagcagcaaa atatgctaat aattccacta ctgtcatttc atcgtataaa ggattttgct
    19981 ccggtaaata gccaatttgt ctgctggctt ttaagggatc ggtaaaaatg ctttcgccgt
    20041 caatttcaat atttccggaa ctgggttgca aataaccaac aatcattcta agagttgtag
    20101 ttttaccagc tccattagga cctaaaaaac cgacaatttc tttatcctga atgcagaaac
    20161 tgatttcatc aacagctttg atttctccga aatatcggct cagcttatct atttttatca
    20221 ttttacataa acattttatt attattggta accggttcgt tcagtaaaat tatatctcct
    20281 tctttaagac cttctatcac ttccacttgc aacaaatcat tggtaccaag ctttacaggt
    20341 gtggcaaccg aattgggagc tgaagatgtt ttctccttgc ttttggtttc tttagacgga
    20401 cttttagtgt ctgtaaccag ataaacaata tcctgattta aatcgttgct gaagacggct
    20461 cggataggaa ttaccaaaac attttggcgc gaatcagcca aaatagttac atttgctgtc
    20521 attcccggtt taacaacttt accgctggca ttaagattaa tttcaatggg aaagacctta
    20581 gcattatttt ctataattgc ctgcggtgca atttttacca ccttgccatt gtattgttca
    20641 taaggaagag catccaaagt tatttctgcg ctctgattca gtttgaattt ggaaatatct
    20701 acttcgttga tgttgctttt tacaatcatc ttgtttaaat cggcaatttt cataatcaca
    20761 gtaccttcac caaaactggt taaactggat tgaaccattt caccttcgtt aatattacgc
    20821 tcaataacaa ctccactggc ggtagcataa acatgagtta cttttcccgg aacatccaga
    20881 tcacgaatca tttcatattg actgctggct tgagtatatt caatttctgc ctcttttaag
    20941 gcatccgatg ccttatcata ttcttcctgc gaaataaatt gtttttgcag aagaattgtt
    21001 ttatcagcca aatctttacg cgcatttttc aagttcattt ctgctttttg cagcattgct
    21061 ttagtattga aaagagtatt tgcctgatta taatctggtt caatatcggc aattatctgt
    21121 ccggaagtaa cataatcatt ttccccggca taaaacttaa caattttccc gcttactttg
    21181 gattttaggg aaaccacaac ttccggttga acttcacctg taatttctat gcgggaagaa
    21241 atatctcccc gtttaacggt ataggtttct gtctctgtag gttttactgt ttcagtttgc
    21301 tttttcttac cgcagctttt tcccaaaaac aaaaccagca gaatcagaag cactataact
    21361 ataattatcc accatttctt acgcataata cctctttttt atagtatctg ataatataag
    21421 acaaaggttg aaatagcaga gattgtgtca acttaaaaaa ggtctggagg tctggaggtc
    21481 tggaggtcaa gaggtctgga gaaaatattt tttggggtgg acttggcgtt ttggtgtttt
    21541 aagtgttaaa taaatctatg ctctctgttt aaatttgaat aatctgggaa agacgatata
    21601 taagacaata agaattttgg ttgacttata ccttgctata aaaaataagg tattttacga
    21661 atatttatgg tgggacaatg aacaagcttt ttatgcccga actggtaaag attgtagaga
    21721 agttcgagaa caaaaatgcc tgcctgcaat atatggcaaa tttgctatcg gaaagcggct
    21781 gtttaagttt tccagaccgt tttctggcag cagttaaagg aagagaagaa ataatgagca
    21841 caggtatagg gaaagggatt gctattcccc atgccaggga cttaacagtt agctgtttaa
    21901 ggatagctgt ctgtttagtg aaacaggatt tggatttctc cagtgtggat aatttacctg
    21961 tgcatttggt ttttatgata gccgttcccc aaagttccaa tcaggattat atgaagattt
    22021 tacggtcatt atcggaattt ttgaggcggg aagaaaacag ggaaacattg ttacaggcca
    22081 aagatgaaag ggatttgtat gaaaaagttc aatttttgga agaagaacta cttcctcagc
    22141 ttggcgttta gtattctttt tctaatgcca ttgctggcac agcatcaaga tgccggaact
    22201 accggttttt ctacattaaa gataatttat tctgccagag caaatggaat gggacaagca
    22261 atgctgggaa gagcaaaaaa ttttgatggt atgcagttta atcctgcatc cataatcaga
    22321 gttcccaatc agggtgtttt tactaccttt gcagaccatt ttgttggttc cggaggtggt
    22381 tcggtaagtt atgtagttcc caaaaatata tacacttctt atggatttta tttaaactat
    22441 tggaattcgg gttctataga caggacggat gtagggacaa atggcgaatt tattgaactg
    22501 aatgatacct ttaatgccca agatatatta gccggcttta ctttggcaaa attcgtaagc
    22561 cctgctttgg atgcgggagg tggtataaaa tttgttttag accaaataga tgataaatcc
    22621 gcttctgccc tcttaattga tgtcggtatt ttgcatcata ctgccaacga aagaataaaa
    22681 gtaggtctat cggcgaaaaa tttgggtttc caaattagcc actacagcga taaaaaatac
    22741 gatgaaggaa taccatttac ttatggtgca ggttttggaa tagatgtggg gaaaaatact
    22801 ctgctgaaca tagatattac taaagccaat ggagaaaact ttatcggtaa atttggagtt
    22861 gaacaacaag ttcatccttc cttggttcta agaggtggat ttaaaactaa tgccgaggac
    22921 tattatctcg gaggaaatat ggaatggact tccggaataa gtttaggagt gggctgggtt
    22981 tggaaaaaaa ttgccttgga ctatgctgtt gcttcttacg gggacttagg attaaccaat
    23041 caggtaagta taagatataa ttttgaataa gtatacaata ataattaatg ataggataga
    23101 atttggaacg aagccggttt ttacttgaat tgggatgtga agaactcccg gaaagacaga
    23161 tagaaattgc ctgtaacggt gtcagctcat cttttgcgac ttttttgaaa agtaataaac
    23221 tatattgcgg ttcttataaa gtgagtggaa ctcctcgcag gatatatttg gatgctctgg
    23281 aactggatac tcatcaggag gatgaagaaa ttttaaagac aggaccttta gtcagcgttg
    23341 cttacgatga agagggcaat ctaacacctg caggtaaggg ttttttaaac agaagccaag
    23401 caaaagaagc aacaatattt attcaggaaa cagagaaagg taagtttctg gcagtaaaat
    23461 atataaaaaa gggattagtg acagttgaac tgctgaagag ctggattccg gaaatgattg
    23521 tgcagatacc atttgagaaa aaaatggctt ggagccaatt aggattaatt ttttcccgcc
    23581 ctctgcgctg gtttttaatt ctatggaatc ataagccctt agagttagat tttgggggaa
    23641 taccttgcgg gaatattact tacggaaatc gttttttggg gttggataag tccattacta
    23701 ttaataaagc tgaggactat cttcccaaac tgcttgaaca gaaagtaata gctgatattg
    23761 aagagcgcag aaaagaaata attacccaac tggcagctat ttttccggaa ggcaatttta
    23821 aagttataga agaccgtgag ttagtagaaa cggtagttaa tttggtagaa tatcctactg
    23881 cggttgttgg agaatttgaa gaaaagtatc tttccctacc ggataaaatt attatcagca
    23941 caataaatca agcacaaaag tattttgcgg tgcaggacaa ccaaggtaaa ttaagtaata
    24001 aatttgtatt tatttccaat ggtaatccat ctgcaagtat gattatcaga aaaggcaatg
    24061 agaaagtagt aaaagcacgc ttggcagatg ctttatggta ttggaatgaa gatacaaaat
    24121 atccgctgga atattggacg aggcatttgg acaaggttat atttcaagcg aaattaggaa
    24181 caatggctga taaaaccaaa cgcataatgc aattggtaag tgccttagcg gatgaattga
    24241 agataacaga agaacaaaag aaaaaggcaa ttcgttgcac agaactttgt aaagcagatt
    24301 tggttactaa tatgcttgga gaaaaagaat ttacaaaact tcagggatat atcggcaaac
    24361 aatatgctat tgcttcggga gaagatcggg aagtcgcaga aggtatattt gaacattatt
    24421 taccaagagg ttcgggtgat gaacttcctt caacattgtg tggagcaata gttgctattg
    24481 gagataaaat ggatacggtt gccggaatta ttggaattgg aatgatgcca acgggaagtg
    24541 gagatccctt tgctctccga cgcgctgcta atggtattgt gcaaattatc agctcccgaa
    24601 aatgggattt ggatttgttc gcttttgctg atagagcttt ggaattaatt ggtaaacaga
    24661 cagaattaga caaaaaggca aaaaagaatt tgcacagttt tctggaacaa agaattattg
    24721 gattgctgaa aacaaacggt atcgcttacg atgtgataga aagcgtgatg catattgata
    24781 aaagccattt gcacgatttg gaaaaccggg ctcaagcatt gaatgcatta aaaggtaaag
    24841 aggactttat tcatctggtg attggtttta agagggtagc aaatattatt gctgaaacac
    24901 aggattttgt tccctttaat aaagataaac ttgttgagcc ggaagaaatt aacctttatg
    24961 aagctctgca aacattacat caggatattg attccgcttt acaaaacaag gattatcctt
    25021 tggctttaca gtatttgatc aagtttggaa aagttataga cgcctttttt gacgccgttt
    25081 tggtaaactg cgaagatgat gaattacgca ataatagaca ctccttgctg aaagaagtaa
    25141 aaaaagaatt tttaagagtt gcagacctat cgttgatagt tctggaaacg gaacaaaagg
    25201 aagcaggaaa atgttcataa tagatgagtt aataaacaaa gcaaaaggaa actccggaag
    25261 tattgttttg ccggaatcca ctgatccacg cgttttaact gcggttgcca aaattgtggc
    25321 aatggacttg gcaaaagtta ccctgctggg tgaaagtgaa ataataaatc aagaagccaa
    25381 agcacttaat atagaattaa acaatgtgac cataataaat cctgagacct cagaagatat
    25441 tcccgctttt gcagaagcat attatcaaag acgaaaagag aaaggtgtta caatggctca
    25501 ggcaatagag acagttaaaa atcctttata tttcggtgct tctatggtta aagaaggaaa
    25561 agttgaagga atggttgctg gagcggctaa tattacagca gatgttttaa gggctgcatt
    25621 acaagttatt ggtgttatgc cggggcttaa aactgtttcc agcgcattta taataccggt
    25681 tcctgatttt cggggggaaa aaagaatttt tttatttgct gattgtgctg tagttcccaa
    25741 tcccagttca gaacagcttg cggacattgc tatgagcact gccttaaccc gaaaagctat
    25801 aatcggagat gagccaaaag tagctttact ttcgttttcc actttaggaa gtgctaaaca
    25861 cgaacttgta gataaagtaa ctatggctaa agaaatttta acagaaagaa aggtggattt
    25921 tgattttgat ggagaattac aggttgatgc agctataatt cccgaagtgg caaaaaggaa
    25981 agcaccccaa agttctgtgg caggaaatgc caatatcctt attttccctg atttacaggc
    26041 aggaaatatt ggttataaac tggctcaata cataggaaaa gtagaagcaa tcggacctat
    26101 catacaaggt ttagcagctc cggtaagtga tttatcccgg ggttgttctg ctgaagatat
    26161 tgttaataca gtcgctattg ttctgctttt ggcggatcaa caaaaaaaga aataaaaaaa
    26221 agatagattg gaggtgaaga tggctatcaa gctatccaac cgctgccgtt taattaagcc
    26281 ctcgccaaca ttaagtttat cggcgaaagc aaaagaaatg aaggatgctg gaattgatgt
    26341 aatcagtttt agcgtaggag agccagattt taataccccg gaatatatta aggcatcagc
    26401 acataaagct atagatgcca attttacgcg ttatactaat aatgccggaa taccggaatt
    26461 gagacaggca atttgcgaga aattattacg ggataacggg ttaaagtata gccccaaaga
    26521 aattctggtt tcacctggag ccaaggcagc aattgtaaat tccctgattg ctgtgtgtga
    26581 tgataaagac caggttttga tggcaactcc ttattgggta agctatcctt atcaggttgc
    26641 cttagctaat gcagaaccgg tttatattcc cacctgtgaa gaaaagggat ataaaattca
    26701 acctgcagac ctggaaaaag caataaaaga gaatccctgc tccaaagtgc tgataatgaa
    26761 ttcacccagc aatccaacag gcacagttta taaccaagct gaactttccg ctatagctga
    26821 gatatgtatc aaatataata tattggttat atccgatgag atttatgaac gcctggttta
    26881 tgatgaggca aaacatatat caatagcaag tatcagtgaa gaaatgaaac aaaggactat
    26941 tgtaataaac ggtgtttcta aagcttatgc aatgactggc tggcgtcttg gttatgcagc
    27001 aggacccggt gatattattt cagcaggagg tatggttcag gaacatacaa cttcctgtgt
    27061 aaattctatt acacaatatg cctgtgtaac agctttaaag gaagaagacg attccataga
    27121 aaagatgcga gtggaatttt cccgtcgccg ggatttttta tatgaagagc tgaaaaaact
    27181 tccgcatatt acctgtttta agccccaagg agctttttac attatgccgg gaataaaatg
    27241 gtatctggag aacaataacc agaatattaa gacctccgat gatttttgtg ctaaattact
    27301 tgacaagtat tatgttgctc tggtatcggg taattcattc ggaatggaag gaactgtgcg
    27361 tttttcttat gctaatagca tagaaaacat aaaggaagga ctaaatcgct ttgcttcttt
    27421 tctggcagag ttagcttccg ggaggtaaag atgaacgatt ttaacgataa gatgaaagaa
    27481 cgctggaagg ataaacgtaa aaaagcatat aattggccta ggctaataat tatggtgctt
    27541 gccttaataa tcattcttta tcttatgaac cacctcggga atacaaaaaa tgtagttcaa
    27601 aatccttctg caagtgttac cgacacttta caagcagata cattgcagaa ggagatcaca
    27661 ccttgagttt agtaatagtt ggttcaatag gtttggatag tatttccaca cctgctggag
    27721 aaatacaaaa tgctttaggg ggctctgcta tttatggtgc tttagcgggt tcctttttta
    27781 cagatgctta tattgtagga gtagtcggaa cggatttccc gatagatgga cttaacttac
    27841 tgcagaaaaa aggaataaat ctggacggac tggagcttaa agagggcaaa acatttcgct
    27901 ggagcggaaa atatagtaac tggaataaag tggaaacgct ttctacagag ttaaatgttt
    27961 ttgctgattt ttatcctcaa ttgcccgagt cctgtaaaag ctgtcgtagt ttattattag
    28021 ctaacatcaa tccttccctg caaatgcagg ttttaaatca ggtatccggt tattcacatc
    28081 ttgcctgtga tactatgaat tattggattt atttgtgtcc caaagaaata gaacaagtgc
    28141 tgaggagagt aaatattgtt tttatgaatg aggacgaagt tagagagtat acaaataagg
    28201 agaatattta ttctgcagcc agagatatac tctctttggg accgcagtgg ataattgtaa
    28261 aacgcggtga atacgggtcg gtagctattt caaatgacga tatgtatttt gcgcctgctt
    28321 atcccttaga agaagttaaa gacccaacag gagcaggaga tagttttgcc ggtgctttta
    28381 tgggatattt ggcaaatcat aatatcttaa accgagcagt aattaaggaa gcaattttat
    28441 atggaacagt tgtatcagcg ttaaatgtag ctgatttcag cgtgaaaggt ttaattgata
    28501 aaaccaaggc ggaaattgat gtgaataagg aacaactggt aaaatggaca acttgaaaaa
    28561 tggactggat tatcgcaaag cgggagttga tatacaagct ggagaagcaa cagttttagc
    28621 aataaaacag aaagttcgta gcacatacaa cagcaatgtt cttagtgaat taggcggatt
    28681 tggcggactt tataaaatag ataaaaattt atggcatcag ccgattcttg tttccagcac
    28741 cgatggagtg ggaactaaac ttttaattgc tattatggct gacaaatacg aaacaattgg
    28801 tcaagacctg gtaaatcact gtgtaaatga tattttagtt cagggtgcaa tcccgcaatt
    28861 ttttctggat tacattggta tcggaaaact ggagccggca aaagtgaaaa aaatgattga
    28921 aggaatcatc attgcctgtc agcaaaattc ctgtgcttta atcggaggag aaatggcaga
    28981 aatgccggat ttgtatgaag atgaagaatt tgatctggtg ggcacaataa taggtattgt
    29041 agaacaggaa caaatattgc ccagacctaa aataaaacct ggtgaccaac ttatttcttt
    29101 gccaagcagt ggattacata ctaatggtta ttccctggta aggaaaattg tgtttgagaa
    29161 actaaaatta tcgcttgata gctatattcc cgaatgtcaa actaccttag gtgaattact
    29221 tttaagtgtt catagaagtt atctaccttt aatgaaagat tatcttactg accctaatct
    29281 taagggatta gctcatatta ccggaggtgg tattatcgga aatttaaaac ggattttacc
    29341 agcagatatt agtgctcaaa ttaaactcag gaaaacagaa atacctcctt tctttcattg
    29401 gttgcaagag gcaggaaaac tatccgagga aacaatgaga gagaccttta atttgggaac
    29461 agggatgatc tgtataattg ataatgaggc atttgataaa tacttaacta taccggaagc
    29521 aagatgtata ggtgaacttg tgaaccaaaa tccgacaaaa gaaaaagtgg aatttattca
    29581 ggaatgttaa agttaagaaa tttagatagt tttcaccttg ttagaagagg aattttgatt
    29641 atattataaa aaaacctctt gacttttttc ttaagattac aaagaagagt ttttgagtaa
    29701 gaaattaaga tagagggaaa agcttatttg agcttggaac tataagtaaa ataaaggtat
    29761 aaaacccaga agatagggag gttttcgatg cgtaaatatg cattgatcct actaataatt
    29821 atgcttgcag gaacagtatt tttagaagct gctactactg gaaaacttgc agttagagtg
    29881 cgcgataatg ccggtcgccc cttggaattt gttaatattg ttgttatgca aggaaatcaa
    29941 cgaataaccg gtggtcaaac gaatgctaaa ggaacagcaa ttatcataaa tattccccca
    30001 ggtctttata cagttaagtt ttctcttatc ggttatgata ctgtggtgta taatgatgtg
    30061 cgtataattg tagatcaaac cacaaatctt gctcctacta tgaataaaag cggttttgaa
    30121 atgaaggcag taaccgttac agctactgaa gataaagtag ataaaaaccg tgtaggaagt
    30181 gccagaaata tagaaatgag tattgtaaca gatgctgctg tttcagatgt tgctgatatc
    30241 gttgctttgc aggcaggagt aactaatgtt ggaggcgaat tacatattcg tggtggccgt
    30301 tcaaatgaaa ttaattatac tatagatggt atgagtgtta ccgaccctgt tgacggagga
    30361 gcgactttac aagttgatac cgatgctatt aaagatatga aagtgatgac aggaggtttt
    30421 ccagcagaat atggcaatgc tcaatcggga gtgatcaata ttgttactaa agatggagat
    30481 ccttttttct ccggcaaggt tgaatataat accgatcatc ttattgggga tgggcgcaat
    30541 actgatgttg tgaaattggc tattgggggt cctgtattac cctttgcttc tcaggaaatg
    30601 aaggaaaaat tcacatttta tctcaatggt gccggtgaat ggttggatgg tcgtttgaaa
    30661 gattattata taagtgatcc caatggtgat tatactttaa atggaagatc gctaattgat
    30721 gctgattatc ctatctataa tccttatacg ggtagggata aaattctcgg tattgatatt
    30781 ggcaaccgca attacaattc ctataatata aatttaaaaa ccaagtatgc ttttaagccc
    30841 ggtcaggtag ctacatttgc cgttcggggc gatagaagtt taaactatcc atatagtaat
    30901 aactggcgct atgcacttca gcactatgca gaagatgaaa taattcagcg acaatatatt
    30961 ggcacttacg attatgtaat taatgataaa atgaatatta aaatgaaagc cagttattat
    31021 cagaaagata gttatgaggg acccaaaggt attgatcggg attcctatat atttatgact
    31081 gtagacccta ataatattcc cagtgattat atacaaaggg tagctttagg ggactatgga
    31141 tattccacaa ttgatgcgga tagagatggt atttatgatt atgggttttt accctatgat
    31201 tattggaaat tccgtctgga aagcgtggaa gagccaagat caattcctgg ctatcttcct
    31261 cccggttata tttatagtgc cttcatagat gataccacta aaatactaag tggacgagca
    31321 gattttgaat ggcaaataaa tgaaactcat atggcaaaga ccggtttaga aattataaag
    31381 cataacatca aaaaaaatca attagaggac tatctgaata ttgatgaaaa acgacgcgtt
    31441 gcctatttaa acagcatatt tgatattaga aaatataata ttgacaattt tgttgatgcc
    31501 gatggcaata tgttgttaga tgatgaatat atccccgagg gcgtttatgc gattgttgca
    31561 actaaagaaa atccagcatc tattgctgat ttggttccta tttataagcc ggaggattac
    31621 tttgctgcag ctaaagcggc atccggaaag cgtgatggtt ataaagcaga gccctggcaa
    31681 atggcatatt acttgcagga taaaatggaa tgggaaggaa tgattgttaa tgccggattg
    31741 cggtttgatt tgtggtattt gggtaaaaaa tattatgtgg cacaggatga tggtagttat
    31801 gcgttacgcg aatttgataa tagtgatctg atgcagttaa tgatttctcc gcggttgggc
    31861 gtttctcatc cgattacaga caaagatgtt ctgcgttttg cttataacta tcagaatcaa
    31921 ttacctcaaa tgcagtatat ttttacgagt aaaactcctg aagatgctta tgcttcggaa
    31981 acaacaatca gggtaggaaa tcccagtttg gaaccacaaa taactgttac ttacgaagca
    32041 ggtctttctc accaacttag cgatgattat gttttagatt taactgccta ttataaaaat
    32101 atatataatt atgtgaacac cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnacattcc gcattgcccg aggagaagaa ttctttatac cttttactaa ctttatattg
    32281 cctctggacg attttagcgc taatatcaat tgggcttatg cttcaggagc accttatact
    32341 ccacaaagtt acgaatctgg tcaagaactt gacacgaata gtaagagaat gccaagtact
    32401 caacaaacaa atttaagaat aaccaagggc tttctcttgt ctaataaaat caatattaaa
    32461 gtttttgcgg aagtggaaaa tctatttaaa aacaagaaca ttataagtgt ctatcccaaa
    32521 acagggaatt attactggaa tggctctcag atagaagaat ccaacattcc cggtttcgtt
    32581 tatcctgagg tttattatgt aaattcctta gcaagtaaaa atccgcttta ttataatgac
    32641 ttccggggac ttaccctggg cttttccttt aacttctaaa ttattccaag gaggaatcaa
    32701 taaaaatgag aaaattcatc ggaacaatgc tgctgattat gatgatgacc tttgtcttcg
    32761 caagttttgc ttacgcacag gaaaaacctg cagctgagac agcgggtggt ggtttacaga
    32821 actttgcaga actggtattt ggcagcggac tggtaaaatg gtttaaaaat ggcggatggg
    32881 caatgtgggc catcctattt ctggcaatat atggcttagc atacataatt tggaagttta
    32941 ttgccttgct caatggaaaa gtaaatctga ataattttct gaataagatt attcccttga
    33001 tcaaggaaaa gaagtttgaa gaagcaaaag aaattgccaa gaataccaga ggtcccgtag
    33061 cagcaatcat ttatgccgga ctggagaaag tggataaagg attgactgcg gtagatgagg
    33121 caattgaaaa tgcttcaata attgaaatga gctatctgga aaagggattt attgaactgt
    33181 catcttccat aactcttgct cctatgttag gatttttagg aacggttgcc ggtatgatta
    33241 ctgcctttga tgctattgca gctgcccgag ctgtggatgc aacaattgtg gcaaccggta
    33301 tccgaattgc tctgattaca accgaagccg gattgattgt agctatccct gttcaatttt
    33361 ttaataatgt ctttatgacg atggtggatg gcattgtttt agatatgcaa agagcttccg
    33421 ataaatttac ggaagtttta acagaagaat tgggtttggc aagatgaaaa tcgtacggaa
    33481 gaggaaatac agagcaacaa taccaactgc ttccacgggt gatattgcat ttctgttgat
    33541 catcttcttt atgtccacta ccaagtttga tgtgaaagaa ggtataaaat tggttttgcc
    33601 tcaagcagca gagtcacaag aaaataaaac taaaaccctt accttaacgg agaaagagat
    33661 gacccgtttt caaatttttc ctgatggaca aatagcagtg aatagagaag caccccgata
    33721 cattgatagc gaagaattgg atgtaataat tcaacaaaaa gtgcagataa atccggatat
    33781 gatcttcaaa gttataacgg atagagaagc aaaatataat gatatgatca gagtgattga
    33841 tcgcttgaaa gctgctaaaa tagaaaaaat atccttatct acaagttagg agcaaaaatg
    33901 gctaaattta gtgggaaaaa aagacgggat gttgtcattc ctcaggcatc tacttccgat
    33961 atagccttcc tcttattgct gttctttatg gtaactaccg tttttgttca ggaaaaaaag
    34021 ttctgggtgg aacgagaacg atggcccttt gcagaaagta ttgaacgtat tcctcgtaat
    34081 catacaagta ctatttatgt aatgcgggat gagacaatcc tgttggatga tataccaacc
    34141 cgcatagaaa gttctgagga tgttttcgtt tcacaaactc ttatccgtaa gaaagaggag
    34201 gaccctgaat tgattgtctg ttttcggaca gatagagaaa caaaatatgg agtaatgtcg
    34261 gatataatgc gtcaactaca agatgccaat gctcttgtag ttgcttttga gacacaacct
    34321 atactaaaga aataaaggga gaaaaatgaa tacaaaaaat caggactgga aagacattgc
    34381 taacgcccaa ttcagcaaag cgcttgcatt atcccttaca ctacttatct ttggtattat
    34441 ggtaactccg aaagttgaag ttagaaaaca ggttttccaa tcacagcaaa tggaattagt
    34501 tgatattcca ccggaggagc gggaacggat tgaggcacct caaacagaag ttgatgttaa
    34561 tgtcactttt cagataagtg atgttttagg gactgaagaa gttgatatgg ctgctttcca
    34621 ggaagcgtta tcccaaattg gcaatattta tagtactact gctccaacgc aacaacaagg
    34681 tgaagatgat acacctgtta attttgttcc ttatgatgat gcacctgtag ttataggaaa
    34741 aattgaacct gtttaccctg aatttgctaa aaggaacaaa ttgcaaggaa ccgtaattct
    34801 ggaagtggaa gttctaaaag atggcagtat tcgtgaaatt agagttcggc gtggagttgg
    34861 tggtggcttg gatgaggcag ctattgaagc agttcgtaag gtaaaatttc aaccgggaaa
    34921 aagtagtggt caacccgtgg actgtatggt aattattccg gtagaattta aaattcaata
    34981 attgggagtg tttaatgaag ttttataaga ttggaatctg cctttgccta ttgctgtttt
    35041 ttacagcaag cgcagcttat gcagctatgg cagttcctgc tacaggaaca aaaaaactgg
    35101 atttaactaa atatcataat gtaggaaaca tctggttacg agttagtaat tatggtttct
    35161 tcggttccgg agatgatatt gttcctcaat atccttcctt ggaatatcct ggaggaagtg
    35221 gaattgatta tttatatcaa ggggctttgt ggttcggagc caaaaaacag cgcagagatt
    35281 ctttcggaaa aaaattgtac tggttagttt atcctccttc ggaaactaac gacactatta
    35341 ttactgacgc agatcctcgc tggaattcta ctaaaaaagc agtgatagat actttggtaa
    35401 gtgtgggttt tgatggtgat gatgatttat atgaattttt acctgcttac aatcctttgc
    35461 tggtaaataa tcctgatcaa acggaaaact attctacata taacaatctt gatggaatta
    35521 tttatgccac tacaaggaaa caaaagcgtg gtgtggatga tgatggtgac gggaaaatag
    35581 atgaagatgg cgctggtttt acctttccct tcagaagcgg ttcagaattg ccagctcaat
    35641 tcagtgagaa ttttgggagt catttcctag cagaggttcc cattagggat tttactattt
    35701 tagatgatcc tataaatgcc gaaatttggt ttcctctggg ttttatggat ttatcatata
    35761 taaattcaaa ttatgctttt gcatcccctt atgacgatga tcaggatggc aggatagatg
    35821 aagatggtgc tcccgtttcg gaacaggatt ttatctcttt ttattacgat tactgccctt
    35881 ttggaacatc cggagagcgg gattacggta aatcaagtgg ggcaaacaga cattatccgt
    35941 taaatatacg agtgcgccag atgagttatc aatggagttt tgaatatatt aaaaatctgg
    36001 tttatgtaga atttgatata accaatatga acgaagccga tactttgtat gactgcgcaa
    36061 tgggaattta tatggattgt gatgtaggtc ctcaaacttg gggtgttgaa aaggcatctg
    36121 atgataagtc cggttatgta aagggaaccg gatatgaatt tgcctatact tatgatgcgg
    36181 actttgataa cggtattact cccggattgg taggagctcg tgtctgtacc cctgatccgg
    36241 aaaagttaaa gttccactgc tggtattgga aagttggcga tggacctagt gacagtaatc
    36301 caattaaaat tcccaaacct ggtgagacct caaatgaaaa atattggtta ttaaccggac
    36361 gcaacccaaa accatcttcc agtatgtata ctgccctgcg ccctgaacag gaagatttaa
    36421 ctgaatggga acaacctact ccaaatgata cccgttttct attttctttc tatggcgctc
    36481 aaccagaaaa tccttcagaa tatagcgatc cggcaaaacg gtggaacttg ccacccagaa
    36541 agacaatgaa aatcgtagtt gcagttttcc ccggagataa taaggaagat ctgaaaaaat
    36601 cagcaggttg ggctaaagaa atatacggcc aggcacaaga tctgattacg gtaactcaac
    36661 cagatacatt tcctcattat acacctcctg agccaccaga tattccaaat ttatatgctc
    36721 aattgcaaga tgatggtaac cgtttggatg tttattggga taatcgcagt gaattttctt
    36781 atgatgttaa aacagtgagt actgcaatta ttggctggca aaatcccgaa agtagtttat
    36841 tgatttcagg tcttgatagt gatcctactc cttatattgc aaataactgg gttgatatac
    36901 ctgaagaata tagaccggga ccacctcctg agcctggaat ggaagataaa cgctggaata
    36961 tgaatgctct ggttaatcct tatacagctt cacgtttacg caaggatttt cagggttaca
    37021 ccttatgggg aagaagtgga agcggaagcc aggaggactg ggaaatgatt aagcgttggg
    37081 ataaggttga tacacctcgt gatcattccg attacactgt aaatagtgct tttcctgatt
    37141 tattcaggga ttttggaggt tatttaggta ttgatactgg tcttcccaat aaaaatgaat
    37201 ggaatgtaga tgtaagtgag tatcataaat tctaccactt tgacgaaaat tatcaacttg
    37261 ttcccaatgg tgatacattt tatggttggc ctatttatac accagatatt gtaataaatg
    37321 ggacaacata taatgaagta accgactggc aatcccttca aaactatgct aacagtataa
    37381 taggacccga tcttgaaacc acccaacttt tgcaagcacg cattttcaaa catcaagaca
    37441 tccctgatca tatattttat gcactatatg atcctaaatt aattccttta cagggatttg
    37501 cttttcctgg aggcgaagga gcagaagccc ctgatacttt ggacttgagt aacctacgga
    37561 aagaaagatt agccagacgc tattacaaat catatattat gtatccccga aaaggcgttg
    37621 aatattatgt tgctttaacc gcttttgata gaggtattcc cgcaaatgat ctgaattatt
    37681 tggaaagcgg aagggatgct gatgctaata tgaaagtatt tttccccggt actttggcta
    37741 aggaaaatat ggataatata atggttattc ccaatcccta tattggcaga agcagttttg
    37801 acggacgcag agaaaatgat gaaaagggcg ataaaagccg tcgtatatgg tttattaatc
    37861 tacctaaacg ttgtgatatc cgcatttata ctttagcagg tgatcttgtt cagaccttga
    37921 aacatgatgg tgccccggtt accgatatca tcactatttc taaagcaagt tctacaggtc
    37981 ttgcagcgga tggtatggaa gcatgggatt tactttcaaa aaataaacag ataatagccc
    38041 ctggtgttta tctatactcc gtagagaaca aagccgacgg caaaaacaaa gtcggcaaat
    38101 ttgtcataat taaatgaggg aggagctgtg aagaaagtat taacacttgt cctgattatt
    38161 acaatagccg gtttaccgct gataattaca gccaagccat ttggtaaaac gggaacggta
    38221 gctttgcagt ttctcaagct tggtgtggat gctcgtgccg taggtatggc agaagcgtat
    38281 acagccgtaa ctgatgatat ttcttctgtt tactggaatc ctgctggttt ggctcctgcc
    38341 tttgaaaatc aggtttttgt ttcgcatact aattggcctg ccagtattat gcatgaattc
    38401 ggagctgcca catataccaa tggggtatct actattgcac tatatggcag catattacat
    38461 atggatgata tggatataac agaagaagag ccatttggac ctactggaga aaaattcact
    38521 tgcagtgaca ttgccatagg agcagctttt gctcaacaat ttaccaataa attctcagcc
    38581 ggactgggaa taaaatatct ccgggaaaat ctgtatgaat ttaatgtaaa cagctatgca
    38641 gttgatgttg gttcaatgta taatacgggt tggagaaata taaaaatcgg gatggcaatg
    38701 cgtaactttg gtcctgacat tcgttttcgg gttgatgatg atgaagacgg caaagatgat
    38761 gaagaccctt ttgatttgtt tgataatgat ggagatggtc ttgttgatga agatggtatt
    38821 gaattggata gtaaaatccc tctcagcttc tctttaggag ttagtggcga cctgatgcgg
    38881 aaaggaaata actattggat cgcatctctg cagttggata atgtaataga ccgtttagag
    38941 acctggaatt tgggaactga atacaagctg ggaaacatat ttttccgatg cggatatcaa
    39001 ttcaattatg atacaaatgg ctttgctgca ggagtaggct ggcaagttcc tacttctttt
    39061 ggaattttta acattgacta tgcctatacc gatatgggtg atctggctga gtcctttatt
    39121 aagggtgctc atcgtgtttc tttaaaaatt aaatattaaa agaatcaata aagattcagg
    39181 aggaaataat gaaaaaaatc ctattagtca tcttaacatt gtctttgctt gtgggcatag
    39241 tttttgccca ggaaatgatt cctactcccg ataacaagaa ggcaatggaa acccctgtcc
    39301 ctaaaaaaat aataccgagc gaacgaacag ctccggtata tacttttacc aaaactccgc
    39361 attctctttt aacttcttat tatgattata tgataggaag ttataacggt ttgcctttac
    39421 gtgtaattcc gtctaatatt tttggaggcg gatatttctt aacctatcat ggacgtagaa
    39481 cttctacggg aacccgtcgt atattttacg cttatcttga tgcgaacggt gatgttatcg
    39541 ttaataatga gattacaaca gaagcaaata atgaaggata tggcactgtg gctgttgatc
    39601 cggtttctgg aaagccattt tatgcctggc atgcaaattc cgatactgat gcggaactag
    39661 aagtgcagtt taccgtagat caattttatg agggcattta tggcttattt acccctcttg
    39721 tagatgctat tgataatcct tcaactgttt atacttccga tagtaatttt actgacgata
    39781 atgaattcat ttggcccacg gctgtaattg gtccctctcc ttatcccgat aagagaagaa
    39841 tctatgtgat agcaagaaac tcagttaccc acacttacgg tccttccgaa aatcccaaaa
    39901 ttgcttatgc tgattttgat gcttttcaaa ttgaacaaaa tgttcctctt acttggcaat
    39961 atacttcaat tcctgaaatg gatgaatgga atgttgatag cgagtggcgt agacccttca
    40021 attccttaac tgcagataat gcaggaaatc tttattatgc cggttttcat tttgctacgg
    40081 aagccgatgg atcaaccgat attgttgagc cggatgtaga tgttttccaa tgtggaaatt
    40141 atggagaagg aacttggttg cggaccagta gttggggtaa cctgcctaca tggaatccta
    40201 ataccagtcc tactgataca actggttttt tcaaaaacca aagcgaatta ccttatggtg
    40261 ataatgatat ttactgggaa ttattttctt cagcttcagg tcatttgaat gctactgttg
    40321 acgatttggg tcgtattcat ttccctgctg tttggagact tgttaccaat gatggttatt
    40381 ggtttcctaa tttccaatta cccaaagaaa ttatctttga tccttctctg ccggaagatt
    40441 ctcagtttag aattaacgat atctggcccc ataaggatcc aactaacgat gtagatgaac
    40501 attatcagtc atgggatagc caaccacctt ggggtgttga ggactttgtt caagaccctg
    40561 atacaggtga gtggtttatt gatatgtatt taagctggga atttccctat tgggatcaga
    40621 atgctcatga tagtgctatg tttttccatt gcaataatat gaaagttact gaaaataatg
    40681 gtcatggaat gttagctatg gtctggcaaa attgcgcacg ggcaaagtgg tataatgctg
    40741 atggtgatag cgactattct gcttgggcaa ataccccaga aatttggatt tctgtttctg
    40801 gagataatgg caatcattgg agtgaaccca ttaaactgaa caatattgag acccctgaat
    40861 ttgccggcat taagccaatg tgggtatatc cagccgataa agttatttat gttggtgaaa
    40921 gtaatggtga gaaagttggt aagcttggtc ttcttttcta cgatgatttt acctggggtg
    40981 cttttgttat tagtcctggg gtcggtccta tgaatgatgg tggacgggta atgtttacag
    41041 aactgcaaat cgtctttccc agcaacgctt cagatgatcc tactgttagc cctgttacca
    41101 atatgttgaa tcaaaactat cccaatcctt tcaatccgga aactaccatc agttttgata
    41161 tgcccaaaac tgctaatgcc aatctatccg tttataatgt gaaaggtcag ttggttaaaa
    41221 cacttcataa cggtattgcc aattttggaa gaaatactgt ggtctggaat ggaacagaca
    41281 ataatggtaa caaagttacc agtggactat atttctatcg ccttaccaca gatggtaaag
    41341 ttgaaacccg taaaatgatg ttgatgaaat aagaaaaccc ttcctcaaaa tgtaacataa
    41401 aaaagctccg gtataccgga gcttttttta tccggaagaa cgatatcctc tttgttccca
    41461 gaaatccgta ttacaaaaga accaatatga aacattgtaa tcggttcttt ccgtattcgt
    41521 aatacgattt ttagcgtgaa atggcacaaa tatctttgct tattctcaag atgcctgctt
    41581 ttgcaggatt ggtattatac atttattttt atcaaccttg ttttttcaac agcaaccaca
    41641 taaaaaaagg acagccaata aaggcagtaa ttattcctac tggtaattcc atagacaata
    41701 tatgtttagc gagcagatca caggcaagaa gaaaaatacc tcccgaccat aatgaaagca
    41761 gatatattct tttctgtcct gaaggaataa aaaagcgaac aatgtgcgga ataattaatc
    41821 cgacaaagcc aattattccg gcataagata cactaattcc aatcactata gaagtgagga
    41881 taaaaatctt acgccttagc gataaaacat ttattcccaa gctggcagca tataaatctc
    41941 cgcaacttaa aatatcaata gctgtggact ggaaaaaaag atagatgagg ataataagag
    42001 ataaccccat cagaattaaa aaacttgtcc attcaccttt agtaaatata tgccccaaat
    42061 tacccatcag ggttcctaaa attagagccg tatcttgctg atgaagatac ataatcaacg
    42121 aaataccagc agaaaaaaac attccggcaa tcacaccagc aatcaataaa cggcttttat
    42181 caaatcttcc tttttggtgt gctagtttcc aaaccagcaa cattgtaaaa cctgcaccta
    42241 taaaaccacc aacaggcatc aaaataatca atccggatat accaagaagt atactgccaa
    42301 aagcagaacc ggaagaaatt cctaaaatat acggttcggc taatggattg gcaagcataa
    42361 gttgatagat agaaccaatt ccggctaatg ccgttccggt gaaaagagtt aacagcaaac
    42421 ggggaattct aacctgagta ataatattcg tatccggttt gcctaatgta agatatacaa
    42481 taataatgct tacaccgatt aagaaccata gaatcagaat caatattttt ttcatttctc
    42541 ttcccgccat tcagcaaaaa tttttgccag ttctttcatt ccttcaatac agcgaggagt
    42601 tgcgcgttga agcaaatcgg gattgatgtc cttctcaaag taaattcttc ttttttgaat
    42661 agccggtata ttttgccagc cctttctgtt aattatattt tttagggtat cctgtgaata
    42721 gcaaatcata atatccggtt tagcagcaat aactgcttca ggatttattc tggcatagtc
    42781 acgttccagg gtagaaaaaa tattatcgcc tccggcaagt tcaataagtt cacctataaa
    42841 tgaagaatca gaaacgctca ttaagggatt tctgtatatt tccagataaa ccttaggacg
    42901 gagtttgcct tgtgcatttg ccttaatttt ttctatttcc tcttttaagt aatttacgag
    42961 ttttacggct tcctcttttt tccggataag agttcctatt ctttctatgt ctgaaagcat
    43021 ttcgttaata cctttaggat aaataacttc cacccgaaaa cccaaatttt tcaggtcata
    43081 agctatgttt tcctgctcca aagaactgca aaaaattagc tcgggattaa gagcaatgat
    43141 ggcttcttta tcaatactgc tgaaagagcc aactatcttt ttctgctgca gagaaggtgg
    43201 ataattacat tcagcagtaa tacctgtaat ttccttttcg gcatttagag cgcaaataat
    43261 ttctgccact tccggagaaa gaactacaat gcctttcttt tgattgtttc ctgtttgctt
    43321 acaggaaaaa gcaaagagaa taataataat tacagttaga attcctgatt tcttcactgg
    43381 aaaattcctt ttttctttta tcctaattta tatttaagtt ctaaacagca ggtcttacag
    43441 ataaagcaac gagaaaatca ctattccctg ggaaagagaa cttactaatt ctatttaaca
    43501 gcttatctgt tagtgcgtta tcctgcccaa aacagttcct tattcatatt tctgttttac
    43561 tcttctaaaa acaagaaata tttttttcct tccgctaaat ttaccgcctc ctgtcaagaa
    43621 aaatgaattg cctcttccca attgggcata atttaatcca tctgatatat ggaaataatg
    43681 cttaatatta tttcccctat tcattaatcc tatattgtat taaaaggggt tgatgatggg
    43741 atatgcctaa gtataaagaa gatatgattt catttgggga atgataagca attttttatt
    43801 cttgcaaaag taggaatctg ctaaatccgt attgctaacc tatacggaaa gaaagtattg
    43861 gtattttttc ggcttttggg cttgacaatc ttttcctgta atttagtaag gtgtttatta
    43921 tagtaattgg aatgatgtaa atataaaaga ttaagcttaa taaagaggtg aattatttta
    43981 acttccagta aattatggga ttccatagca ttgattatgg gtctgctgat aagtttggta
    44041 ttaattgcat ccctcacgca gatcatagtt ggtggttcag ttcttgatac attcagtaaa
    44101 gtaactttac gaattatcaa tattctgttt ggaatttcca tagtgttaat tatcctcatc
    44161 gtggtaatgg aaaatggttc tccggcaaca actatggctt ggattttagt gctcattttt
    44221 ttaccggttg tgggctttat attgtatctt ttttttggca gaaattggcg aaaaaagcgc
    44281 ttattcagta aaaaaggata tgccgatacc aaattgctgt tatcttatgt tcacaatcat
    44341 ccttccgata gagaaaacaa ttggcagaac gacttgactg cacgacttta taacttattg
    44401 aaaaataata gtaaagctac tttaacggta aacaacaatt taactctata taacgatacg
    44461 ggaaaagcat ttaaagcaat tttggaaggc attaattctg cccaacattt tgtccatcta
    44521 gaatattttt ccgtttttgc cgatgaaaca ggttacactt tgcaaaagtt attgattcaa
    44581 aaagcactgg aaggtgttga agtgcgtttt atttatgatg atgtaggttg ctggatgctg
    44641 aaaaaatcat ttaaacagga attacgcgat gcaggagtgg aatttgttcc ttttatgcct
    44701 gtctggatac cttttttaaa cagtcgtttg aattacagaa atcatcgtaa actggtaatt
    44761 gtagatggta aaaaggcata tttgggcggg atgaatatcg gagataaata tttgggcaaa
    44821 aaacgctatt atggctactg gcgtgattcc ttggcagaaa ttgaaggaaa agcagttaat
    44881 gctttacaag caatcttttt agcggactgg aattttgtca ccggaaaaaa tttgatgcaa
    44941 gaggacttac tgcataaata ccttcctgtt atttctcctg ataaacaaag atttctaccg
    45001 atgcaaattg tagctgatgg tcctgatagt gattatgaca gcataatgca attatatttt
    45061 gcagcaataa cttacgctaa acatagcatt cggatttcta ctccctactt aattttaaac
    45121 gaaagtttac tttccgctct aaaaactgct gctatcagca gtgtaaaagt tcagatactg
    45181 gttcctgata agcccgatca ttttttggtt tattgggctt cacgcagtta ttttcaacaa
    45241 cttttagatg ttggagtaga aatttggact tatcataagg gttttaatca ttccaaaata
    45301 ttgattgtgg atgacgaatt tctgctaatc ggaactgcca atatggattt acgcagtttt
    45361 aaacaaaatt ttgaactggc tgccgcaata tacgataaaa ttgtttgtgc tgaagccatt
    45421 aagcaatttg atgaagacct gttatccagc ggtaaaatta ttcctgagaa attcagacag
    45481 cgcagtatgc tgcaaaaaac aaaagaatcc atctgccgtc tggtttctcc attgttataa
    45541 catcagggag gttaaggtct taagtcagca gttatcagtc ctcactacca aataagtttg
    45601 tgagtaatga aaaagttatt tcggcagtgg cgactactga cttaaaaaaa ggagaaaaag
    45661 ttatgaagtt tatcaactat cctttatcag agaatctgga tacaatagtc caagaacttg
    45721 ctaagccaga ttccattcta attttcccaa ctttaagcgc agcaaaaaga gcttccagag
    45781 aatatttaat aaattgggac ctttcctggc gcggttttta cagtatggaa gaattgcgtt
    45841 ctgctcttat tttgccaaag caacctttac tgacagatga aaaacgactg ctttgcctct
    45901 ttatggtaat ggaagaaagc gaaagagaat catttcatat ttataattat ggtgatattg
    45961 tggactgggg aaaacgtttt tttgattttt ttgaagagct ctgtgaagaa tgtatagccc
    46021 tttccgatct tgaagaaatg gcaaattccg gagcttttcc gatgcaggaa tggcaagaga
    46081 tttatctgca gaagatttta aatatccgca gtaactatca aaaatacatc aatgctttgg
    46141 gtttcagtga tactattttt tacttgaatg ccgataatat ttccgttccc tggcagaact
    46201 gtgatattta ttatatcaat caatattatt acagtagctt ggaaaaacaa cagataaaag
    46261 ctctggagga ttctggaaac agggtaacaa ttattactca cggtttggaa acggaaagca
    46321 atggtgaaaa ctggaaaata aaagatttca atttacagga atcctggaat tccttgagaa
    46381 agaagccctc cattgaaatt atagaaacgg acaatgaaac ccaaatggca ttatcttttc
    46441 tctcctggaa tttggagcaa gattgtcata attgcgctat tattgacagc acctttcact
    46501 tgaaaagcta tagtcattac tttcccgaag aacattttgc caaaccggaa agctattctt
    46561 tctgcgaagg gactatttac aaaatgcttt ctgcaattgc cacaggactt aaagctacaa
    46621 aagatacaaa tggttatctg cccataaaaa tattggcaaa ttatatctcg gaagattggt
    46681 tctgccgtta cttttacgct gaacaaggta acctaaaact ggatgactat atcagtcaac
    46741 tgcgtctgga gcttggtttt ttaattaatt gggattatct ttatgtggac tatgctttgt
    46801 ttttaactgc agatttgcca accctgaaat ccctcgtatg ggaatattac aatttaatca
    46861 atgccttcgg caaaatccaa aatctgaaag aactctgcaa cctgattgat agtcccaatt
    46921 gcttacagct aaataaattg ctggaagata acgaaaaact ctatactgat attttacctg
    46981 ttttttggga gagaatggca aactttatgg ctattgaaaa tttgggcttg gtaaattcct
    47041 ggaaacaaat ttttgctgaa gacagcattg gcttaaatct gcttgatttg cttttgaatt
    47101 ttcttacaac cggcaaaatc agttaccaaa gaaatgaagc tctaactccc gagtgggaaa
    47161 ttagtaattt gctggatgca agaaatagaa gtttttccac cgttgccttc tttcaaatga
    47221 ttgaaggcat tattccttcc agtccaactc cggtttggtt attcaatgaa tcacaacgag
    47281 caaaattagg attaaaaaca tttaacgata tcagaaactg ggaacgctat tatttctgtc
    47341 gtcttttact gtgttcagag cgcgcaattt gtttcagtta tataaatcag gaacgcgata
    47401 ttagccccag ttccttttta ggtgaattgg aagaactgct gaaaaatgaa gaactggatt
    47461 tatttaaaaa aagaaaaatt cttgtttctc tgccggatgt ttataattgg gaactagtta
    47521 ataagacaat tcccgaactt gaagatcaag aaatttgcaa tattaataaa gacctaactg
    47581 aggatttctt tataatccct tccgatccta ttgctgattt cccaatgaat aatcagatta
    47641 attttagtgc ctcagccctt atccaatttt tgaaaaatcc cttcctctgg tttgtagaaa
    47701 ataaaagcaa actctcaata cagaattggg aagccgaaga aaccataagt tataaactct
    47761 tcggtaacat tatgcatact tatttctcta ctaccttagg tgaattgaaa ggaattcacg
    47821 aatcccacac aatattggaa accctctttg gtaatagtaa aaaactggag gaacaactaa
    47881 aaaatgtaat tgcttccgaa aaaatgatat atcaaatccc taagaactat aatgccgttt
    47941 ttttgggaga aatcctggca aaaaaattat ctgaatccct cttctttttc tatcaggact
    48001 ggctgaaaaa acaggttgaa ggaagaaaat ttattctcat tcccgaagag gataaaatta
    48061 gccctgcgga aatgaaatat aaacaactgg gaaaggtcaa ttggggcaat aaagaatata
    48121 ttattggcat cagaggaaaa gcagacctgc gtattgaact ggaaaataaa gctctcattg
    48181 tggacttcaa aacaggcagt catgactatc gtcaactctg catctatgaa tggtattatt
    48241 atcttctgga tgatgtttcg gcgccaaatg atgtttcttc catattttgg aatattttag
    48301 accctacaga taaagtggaa ggcgtgaatg aagaaaaacg caataaactg aaacagcaga
    48361 ttttggataa tctggaattt tgtcttgcaa atggttatag cacaattacc aaagccactg
    48421 atagacagcg tttgcaaaat attacccggg ctgatttgat aagcagaaaa ggaggtttaa
    48481 aatgactgaa aataactgca gaataatttg tgccagtgcc ggaaccggaa aaacctaccg
    48541 tctttctgta gaatatcttt ccctaatctt aaaatattac ggtaagaatg aattctcttt
    48601 ggataatatc ctcgttttaa cctttacacg taaggcaact gcagaaatta gagatagaat
    48661 tattaaacat ttggaactat tggtttctga accggaaacg gaagaagaac aaaagaacca
    48721 acaagaatta ctactcaacc ttagaaaact taataaaaca aaagaaatta ccttaagtga
    48781 acaagaaaga aatttactgc ttagtgcgcg caaggaatta atctgtgatc aaagccattt
    48841 gcaagtgatg actattgatt cctacacagg acagatcttc cgtaatattg tgcgtccctt
    48901 aaaaaatatt gaacattatg aaatagatac cgaagccgta aaaaagatta tgcccttttt
    48961 gcttgatcat ttaatggaac cggatttaag ggaaaaaatt aaaaatttat taagtaggaa
    49021 agttagccgt tccttagatg attatactaa atttttctgc tcattaattg aacagcgctg
    49081 gcttttttat atgatcacta aacgccaagc agttcataat tcttctttaa acggaacttt
    49141 aatgaattta aatcccgaaa ccgagccagc tgaattctac acacaatttc tgaaagaaat
    49201 gcaacggatt atagcattag tagctaacat ctgttctgat ttgggtaaag catctctgga
    49261 ggattatttt aataaggact ttagaaattt agctgtgaaa ggtgccctaa ctcctgttgc
    49321 tattttcaat aacttaaaac aacttaattc ggatgaggcg gaaaaactac tatatatatt
    49381 agataaaggc actatctgga ataaacagaa aataagcaac aagaagtttg aagccgaaaa
    49441 tattgaactg gaaaatgctc aaaatgaagc caaacaccat ttggctgatt atttaatctg
    49501 gaaattatat cttcccgaac aaagagaaat tgaggaactt tggtcaagtg tattggaaag
    49561 atacgataat ttgatatacc gctataaaaa tttgacctat gatgacataa cctggtttac
    49621 ctttgaaaca ctctttagtt cagaaccgcc ttttctaaat cccgaagatg cagtttcagc
    49681 aacggaattt tatcaatttt taagccatag aacccgtttt ttactgatag atgaatttca
    49741 agatacttcc ctgattcagt tcaatatatt aaagccaatc attgaagaaa ttactgccgg
    49801 agaaggttcc aaaccttttg ggggcttgat agttgtaggt gatgaaaaac aatccatttt
    49861 tggctggaga ggaggtgaac gtgatttact gctgaatttg aaagatattt tcccagcttt
    49921 gggagatctt acagttgaag cactggataa aagctaccgc tgtggtccaa ctttaatgga
    49981 atttatcaat caggtcttta tgaatgcaga attgtgtagt ttactccaag aaaaagaaat
    50041 gcaatgggat tatacacata ttgaaagtgt agaaattaca aaagaaccgg gaaccgaaat
    50101 tgaattctgc ttaaaaaatt tttctaccag cgttgccaat gccgataatg aaaaagatat
    50161 ttacacggat tttgtagaaa gaatggtttt acctgctttg gaaaaagacg attccggttc
    50221 aattgctatt ttatgcagaa ccaacgaaga acttacaaaa attcaacaag cattggatat
    50281 aagtggcaga gctagtttat ttcaacccaa caggtctatt atagaacatc actatgtagc
    50341 tcctttgctt tcctggttgc gttttttagc ctggggtgat tggatggatt ttatctcttt
    50401 tctgcgttcg gactatgtgt taatcaatac aaaagttttg aaacaaaccc tgaatattat
    50461 ttcccgaact ctggaaatac aaaatgaaag acaaaaaccg gtaaggcccg atttttcaac
    50521 tatacctgtt gtaaatgaat tctaccagct ctcttgggag caggaaaaca aaaaggcaac
    50581 agaaatttgc agagaaataa tagacctctg tttaccttcc aaacagctaa gtgaacggga
    50641 ttatttgaat ctacaccgct ttctggatat tttatccaca cgccaattaa cggattccga
    50701 taaaggaatt tcttttcccg atttacttac ttatcttaag gataatgcca atgctgaaga
    50761 ttttaaacag gtttccataa atgcagatga cagtttacaa ctgctaacca ttcataaatc
    50821 caagggcttg cagttcaaaa gagttttcgt tttctgtaac ctttccggaa aacatagcac
    50881 agatagtaat accctaaatt gggctttgga atatgaagga aataccttta accggattcg
    50941 ggattacggt atcagcttgc attatcagaa aatcctgaag tcctcaagct acgccaatct
    51001 ctatctccaa aaccaaaaaa gaaaacagct ggaagaattn nnnnnnnnnn nnnnnnnnnn
    51061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51121 nnnnnnnnnn nnnnnnnnnc gccatcacag acccgtaaat tggaataaat tagttcccgt
    51181 agaacttcct gcagtaaaag atctgaaaac atactatcta acggaacgcc caaacctgat
    51241 cggggattta ttgcatttct atatgtcctt cattatccgc aacacaccaa aagaacataa
    51301 ctatgcttat tacagatgcc taaaccgcta tggagctatt tttaccgtct ttcaaattga
    51361 gcaactggct gatcgctgtc aaaaggtctg cgattctaat ccctatcttt ttacttccgg
    51421 ttgggataaa atctttactg aacaggaaat tctgggggat agcaaaatta tgagaataga
    51481 ccgtttgatg gttaatactg ctgaaaaagt ggttctgatt gcggattata aaagcggaga
    51541 tatttacgat tccaatcaac ttacaaacta taaaacagct ctatccaaac tgtccgtttt
    51601 gaagggctat aatattgata ccaggattgt aattgtgtag tcaagaggta gagaagtcaa
    51661 aaatgtgtaa ccagaggact acaaaacaaa agtattgact aaaacatcaa gtaaaaagaa
    51721 aaggataaat tgagaaataa aagaaaggat tagcaatgct gaaagccaaa atttatgttc
    51781 aacttaaagc taatgttctt gatccgcaag gtaaggcagt tacaaattct ctgcataacc
    51841 tgggttacac ggatgtgatt gaaacccgta tcagcaaata tatagagatg agttttagga
    51901 atgataacag agaaaaagtc agccaggaag tagaaaaaat ttgccaagac cttttggcta
    51961 accctaatac cgagacatat cagtacgaaa taatttcaga ggagtaattt cttgcgtatt
    52021 agtgttgtta cttttcccgg ttccaattgt gattatgata tttacagagt ttgcaaaagc
    52081 cggggtcatc aaaccaaact tatttggcat aaggattccg atttggaaaa gccggatttg
    52141 gtaattttgc caggtggttt ttcctacggt gattatcttc gttgtggagc tttagccagg
    52201 ttttctccca ttgtgcacga agttatcgct tttgcccgaa aaggaggttt attagttggt
    52261 atttgtaatg gttttcagat tttaacggaa tgcggtttac tacctggaac tttaatgatg
    52321 aatgaaaatt tacattttat ttgtcaacat caatatattc gggtggaaac agaagccagt
    52381 cctttcactc aaggcattgg taaaggaaaa gttatggata ttcccatagc gcataaagaa
    52441 ggtaattatt atattgatcc cgagggctta aaacaattga tagctaacga tcaaatactt
    52501 ttccgctatt gtgataaaca gggaaatata aacattgcaa gtaatcccaa cggctcatta
    52561 gataatatag cgggaatttg taataaacag cgcaacattt tagggatgat gccacatcct
    52621 gaacgttctg ctacggaaac agttcccact aaagatggaa ggtggatttt tgctgccatt
    52681 gaaaatgctc tggaataaaa tcttagaact tctgattcct cctgcctgtt tggtttgtgg
    52741 acacagaata gatgaccgtt atcaggttat ttgtcctgat tgtgaagcga agctatatct
    52801 aattggagaa ggaacttgcc ctgtttgcgg aagtgaaaat aaagaacttc cctgtgaggt
    52861 ttgtgcggaa ggaaattttg cctttgattc cgccatttct gtttttcgct attcaggtcc
    52921 ggttagagac ctgatacata ttttgaaata taatggctat agttctcctg ccggttattt
    52981 tgctttgccg atagcagaac ttattgaatc caaaccagtt ctaaaaaatt acgactatat
    53041 atgtgccgtt cctttgcata gagtaagaaa aagagaaaga ggttacaatc aatccgattt
    53101 aattgcttat tctgtggcta aattgattga tatgccatat tttaatcctg tgaaaagaag
    53161 aatcaatact ttaagccaaa ccctactttc ccgtgatcat agaataaaaa atttggctgg
    53221 tgctttccag gtaaaagata aaaacctcgt agcaggcaaa aaaattattt taattgatga
    53281 tgttttcact accggcagca ctttaaatga aattgccaag accttgcgct ctgcaggagc
    53341 agataaaatt tgtgccataa ccgttgcccg agcttaaaat gaataaggaa ttttgggaaa
    53401 tccgacctga gacctcagaa agcgaaatca atgaaatgat tgaaaaggtg gcaaaattca
    53461 ttgccgaaag gcatttggct cctgcaggaa ttcttctatt tgaatctttt cgtcctttac
    53521 acggaattgg aagtcaagca ctgtttttta ttcttccggt agcagaaatc atttttgatt
    53581 ccaaaaaata tcagcagttt gccattatga tggaagatga aaataatata aaaaaactca
    53641 ttaacaggat ggacgagctg gatgaaattt atactcgtga acgcagacag gaaaacaagc
    53701 tcaaaaaaca gcatcgcaaa gccaaaagaa aggcatttta ccacaaacta tttaagaaaa
    53761 taaataaaac tgcttgaaaa agtggaggat atatgcaata cgatgagaaa cgccttaccc
    53821 gtattgttgc tactgactgt ggcagcacta ctaccaaggc aattttaatt gaatggatag
    53881 atggagaatt tcgtcaaacc attcgcgggg aagcacctac aaccgttgaa gcacccttaa
    53941 acgatgttac aaaaggtgtt attaatgcca ctcaggaatt ggaagaattg gcacgcctaa
    54001 agtatcacaa tcctaatatc aaatttatgg aaaacggaga attcgttatt ccccgtaaag
    54061 gtgatgttgg cgttgatgct tatgtttcaa catcttccgc agggggtggc ttacaaatga
    54121 tggttactgg agttgttgct tctatgacag gtgaaagtgc agaaagagca gctttgggag
    54181 ccggagcaat tgttatggac cttattgcca gtaacgataa aaggatgaat catgaaaaga
    54241 ttgaacgcat ccgcgaactc cgacccgata tgattttaat ggcaggtggt gaagatggtg
    54301 gcactgttaa acatgtggtg gaaatggctg aattggttgc cagtgcagac cctaaacccc
    54361 gtcttggttc cggttataaa ctgcctgtta tttatgccgg caataaagat gcccgggaag
    54421 aaattattaa aactctcggc gaaaaggtgg atttgattat cacggataac attcgtccca
    54481 aactggaaat tgaaaatctt cttcctgcca gagaaaagat tcataacctc tttatggaac
    54541 atgttatgaa acaggcgccg ggctataaca aactaatgga atggacggtt ggtcctgaac
    54601 acgaacaagt tcctattatg ccaactcctg cagcagttgg aaatattatg caagcaattg
    54661 ccaaagaaga aaatattgaa gttgtaggtg ttgatatcgg tggtgccact accgatattt
    54721 tcagtgtttt taccaaggat ttcattttta atcgcacggt tagtgccaat ttgggtttaa
    54781 gctacagcat ttccaatgtg ctggcttcag caggactgga aaatattatg cgctgggtac
    54841 cttttgacat taatgaaagc gagctgcgta atatgattaa aaacaaaatg attcgtccta
    54901 ctacgattcc ttctctttta gaagaacttg ttctggaaca ggcaattgcc aaagaggcat
    54961 tacgattggc ttttgaacag cataagtcat ttgccagttc cttaaaaggt atgcaaaaac
    55021 agcgtgatat ttcggaagca ttctcgcaat ccgtttccgg agaaacaatt gtaaatatga
    55081 tgactcttga ccttttagtt ggtagcggag gagttttatc tcatgctcca cgcagaaatc
    55141 agaccgtgat gatgttaatt gatgcttttt tgcctgaagg aataacccgt ctttccgttg
    55201 atagcatttt tatgatgccg catttgggag tcctatctga aatcagcacc aaagcagcta
    55261 cagaggtttt ccgcaaggac tgtatgattt atttgggaac ttgcgtagcc cctgtaggaa
    55321 aaggaaaaat tggtaaacca gctctctatg ccaaactgga gcttcctgat ggctctgttt
    55381 ttgaagaaga tatacccttt ggtgaaatgc gtttaatccc ttgtgagatt ggacaggttg
    55441 ccaaagcaac tctgaaaaca catagtggct tgattttaga tgcagataga aagaaagaac
    55501 tgaccgttga cctgcacggg ggaaaagttg gcatcgtttt agataccaga ggtcgtaaac
    55561 cttttgttct tcctgcagat aaaaatgaac gcatttctgc ccttaaaaag tggatgaaag
    55621 tcctgaatgc atacccggaa catattttgt cataggagga taaaaaaatg ggacaagcat
    55681 attctgccgg tttaaccgtt accgataata caatcttacg caaagaacgc attctgcctt
    55741 tgaaaggaca ggtgctcgtg aaaaaaggcg ataaagtaaa agctgaagat gttgttgccg
    55801 aaaccttact cccaggtaaa gttatacctt tcaatcttgc caataaactt ggagttaccc
    55861 ctcaacaatt agttaattac attaaaatta aaccgggtga taaaatcact caaaaaaccg
    55921 ttttggcgga aactaaagga cttttcgggc tcggcataat gaaaacggaa attctttccc
    55981 ctattgaagg agaagtggaa aacatttctt ccgttacagg ccaaatctta ctgcgtgaac
    56041 cccgtattcc tgttcaggta aaggcattta ttgatggctt ggtaaccgat gttattgaag
    56101 aggaaggagt aatcattgaa aataaaagtg cctatattca aggaattttt gggctcggag
    56161 gagaaacctt tggcgaatta aaaatgatag cagaaagccc cgatgatgaa ttggatgttg
    56221 ccaaaattga tgcatcctgt gccggtaaaa taattgttgc aggtgctttt gcaccctatc
    56281 acgcaattga tatagcccgg aaaaatggcg taaaagctat tattacaggt ggtatagacg
    56341 atcaggatat taaaaaattg ctcggttatg atattggagt agctattaca ggacacgaaa
    56401 atatcggcat tactatcgtt tgcacagagg gttttggcag aattaatatg gcacaaaaaa
    56461 cctttaattt attaaaacat tttgagggtt ataaaacctc tattcatggc agaactcaaa
    56521 ttcgtgctgg agttattcgt ccggaaatta ttattccgct cgagtttgaa gaacaggaac
    56581 tcgttgctaa agaagtttct atgcctattt tggaaatcgg aaccgtaatc agaattatcc
    56641 gtcaacccca ttttggacac attgccaaag tgactgctct gccggaagaa cttactaaag
    56701 tagaatcaga aactttggtt cgggttttgg aagcggaatt tgaaaatggc acaaaaatta
    56761 tcgttccacg tgctaatgtt gaagtaatag aatcctgagc aaacataata cct
//
LOCUS       DAOOZV010000003        53595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_720_length_53595_cov_57.897889, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000003 DAOOZV010000000
VERSION     DAOOZV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 53595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53595
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_720_length_53595_cov_57.897889"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1017
                     /locus_tag="PLW08_00645"
     CDS             391..1017
                     /locus_tag="PLW08_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51147.1"
                     /translation="MKKLLMIAVLLFVTCLSQGQTQSSKTKEESRTTGLGFHFGTVSG
                     NGFSYRKYYNNKSGLQVTLGGFTSGSKEIDSSIYGNYNGASTITITENGKKTKLSSAI
                     NYVRTLDTNQVGRFYIVAGGSLLSSFIRQYEIDYQIVNKAYYTIIKDHPERKKWDSEY
                     AYYFGFGLGFDFNMGQNFHCAVELPLTIDENSEIRMYIPQAGIYYFFK"
     gene            complement(1202..1618)
                     /locus_tag="PLW08_00650"
     CDS             complement(1202..1618)
                     /locus_tag="PLW08_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPL51148.1"
                     /translation="MKSYRRELHFRTDKRREFINITPLVRNVVHESGIKDGLCLVNSM
                     HITSSVFINDDEKGLHSDFETWLEKLAPEKPYSQYRHNGFEDNADAHLKRQIMGREVV
                     VAITNGELDFGTWEQIFYGEFDGMRDKRVLVKIIGE"
     gene            complement(1615..2319)
                     /locus_tag="PLW08_00655"
     CDS             complement(1615..2319)
                     /locus_tag="PLW08_00655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPL51149.1"
                     /translation="MTTKPQIKAIIFDLDGTLIDSVVDIAGAMNAALKELGYPEHPVE
                     AYKTFIGDGHMELARKVLPEDKRTPENIEALAKKFWDHYDIEWYLHTNIFPGVLYLIQ
                     LAVARKMKLAILSNKPHYFTKKMIRHFFRGAMIRHTKNPFGVYSGEEPNKPKKPDPTV
                     ALELAQRLIVKPQNVALVGDSVVDIQTAKNAGMIAIGAAWGYGNKKDLQAAGADLIFD
                     SPTEMSTYLDSQPLCP"
     gene            complement(2348..3184)
                     /locus_tag="PLW08_00660"
     CDS             complement(2348..3184)
                     /locus_tag="PLW08_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPL51150.1"
                     /translation="MLLAINVEKLTGDFITPERISLIIRVILILVVGIPLVRLIRSIT
                     KKIVKNRLSPQSEQLIVRSVYYVAILILFITLLNEFGFRLSAILGAAGIFGVAIGFAS
                     QTSFSNIISGIFLISEKPFKVGDVVQVSSNIGTIESIDLLSIKLKTPDNRYVRVPNET
                     MIKTEVINITRYPVRRVDINLSVSYDDDLHKVKEVLLELAESNPLALKDPAPLFFIDK
                     FGDSGIQILFGVWTKKEDFQNLKTSLIIGIKEKFKQENITIPFPHIFIANAEELKNES
                     TS"
     gene            complement(3442..4041)
                     /locus_tag="PLW08_00665"
     CDS             complement(3442..4041)
                     /locus_tag="PLW08_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPL51151.1"
                     /translation="MKKGLIVLLAIILVIIICAGWFIGRYNNIQKEKVNVESAWAQVQ
                     NVYQTRYDLVPNLVEIVQGAANFEKSTLTQVTEARAKAGGSLNLPPDALTNPQAFQAF
                     QQSQAGLSDALSRLMVVVERYPELKANQNFLAFQSQLEGIENRIRTERMRYNDAAKKF
                     NQLIVTFPNNIIASIIKVKPFQFFQAEESAQKAPKVQFD"
     gene            4475..5683
                     /locus_tag="PLW08_00670"
     CDS             4475..5683
                     /locus_tag="PLW08_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51152.1"
                     /translation="MKKSQFCLNLICLGLLLIPVGLNGLETKLSGEFWVRWISEIAKR
                     KDSNGNYDDKLVSNYLSLERGYLGLETKFTEQVKGRFTVDIFTTDKTYEYQVYSLSDG
                     TLTSSTKSGSIDGAGLKIKYAYVDFGGFIPVKDMTLTAGMQKVYFGSIYDWSYTLIGK
                     APSDEHKVVNSSDLGITLNGFFPSGFGEYQLGIYNGEGYKKIGANLKENTDFAYLGNL
                     RLTPIAGLTVGGSYMLNTVGRDKNLDGETVNTSYEEQKLTDIFTRLAYGPVDLNCEYI
                     VKNVNFPNITDSGKTKEYSATGISIFPCINLKAYLPYDLQLVGCYDVWDESDRLETDK
                     ARSKLNAFTVGINYNFLPDETDNPAMQLQLNYTDKKYDEDKSHSDYADGKKDSSQIYL
                     QLKWKFANTL"
     gene            5700..6518
                     /locus_tag="PLW08_00675"
     CDS             5700..6518
                     /locus_tag="PLW08_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPL51153.1"
                     /translation="MKTKNYLISIIIMLLAAFNLNAGKGNQITCTGSTTVLPIAQATA
                     EAFMNSHPDINISVRGGGSGVGVAALQNGTTDICNSSRPLKSKEISAAKAKGINPVSY
                     AIANDAISIIVHKDNPLKDISIQQIKDIYTGKIKNWKVLGGPNLPIVVISRDVSSGTY
                     EVFNEKVLAGAKVEGSAQLLASNNAVLSSVSNTPGAIGYVGLGYVNNSVKLISVNGII
                     PSEATVKNGSYMLSRKLYMYTNGKAKGDVANYLGFIQSEKGQQIVQELGFINIK"
     gene            6586..7455
                     /gene="pstC"
                     /locus_tag="PLW08_00680"
     CDS             6586..7455
                     /gene="pstC"
                     /locus_tag="PLW08_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPL51154.1"
                     /translation="MSRYKEKAFTTITYTAALFSIMVLFALIYGLFREGLPLFNKVSL
                     TEFIFSSSWYPTNAEPEFGAWTFIIGSFCVTIGALIIGIPLGLGSAIFISEIAGNWLK
                     EIVKPVIELLAGIPSVVYGLFGMSAIAPLIRNWLNLDTGLNIFTASFILGLMIVPIIA
                     SMSEDALTNVPKSIREAALALGSTKMECIFRVIIPAARRGIVGSILLGLGRAIGETMV
                     VLMVAGGSAQIPKSIFDSVRPLTSTIAAEMGETVMGSLHYQSLYALAILLFVLTFVIN
                     LITEFFLAKRDTK"
     gene            7452..8306
                     /gene="pstA"
                     /locus_tag="PLW08_00685"
     CDS             7452..8306
                     /gene="pstA"
                     /locus_tag="PLW08_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPL51155.1"
                     /translation="MKNRKVLNYCGNGLLFLLVGITILVLCIFLVRVFSLGGKVLSWE
                     FLFTAPRNCMQEGGIFPALVGTFWLTVISIGIVIPFGILTAIFLTNYGKPKWLLKVVQ
                     IAIDTLAGTPSIIFGLFGMAIFVNFFAMNVSLISGALTLAILALPLFINSCIEAIKSV
                     PFDFYEASLALGATKRQTITKIVLPTALPNILTGVIVCIGRVAGETAPIMFTAATFYT
                     RRLPKGLKDEVMALPYHIYALMTEGTHPETQVPIAYGTAVVLLLLVLGVSAAAVIIRS
                     SIRRKRKW"
     gene            8300..9058
                     /gene="pstB"
                     /locus_tag="PLW08_00690"
     CDS             8300..9058
                     /gene="pstB"
                     /locus_tag="PLW08_00690"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPL51156.1"
                     /translation="MVNNILSTENLNLWFGRNYVLKYVNLNVLDKNITAVIGPSGCGK
                     STLLRCFNRMNDLIQDTHIEGKVKLLDDDIYTSKMDVTEIRSRIGMVFQKPNPFPKSI
                     YKNVAYGLQIQGKYHKDEINERVEQALKAAWLWEEVKDRLHSPAMALSGGQQQRLCIA
                     RALANRPEVLLLDEPTSALDPQSTSKIEELCLELKKSVCILIVTHNIAQAGRISNYTA
                     FMYLGELVEFGPTEKVFTVPADKRTESYLTGIFG"
     gene            9080..9736
                     /gene="phoU"
                     /locus_tag="PLW08_00695"
     CDS             9080..9736
                     /gene="phoU"
                     /locus_tag="PLW08_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPL51157.1"
                     /translation="MLIERISELKQLLMAEAGLVEKMILLAMDGLANFGTAFLEEVNT
                     FETRVNQIEIELDEKCMTVIALYQPEAKDLRQILMIYKINNDLERLGDQAVNIAESSA
                     QLVGSPVLQLIPELFAMKEAALKMLKDALFAFSGENYELSRQVCDADNIVDEYNRNIY
                     KHLVKLIRENPLQTDLYLHILRIAKNLERIGDLSTNIAEDTIFLSRGKVIKHRIDNIP
                     "
     gene            complement(9900..10427)
                     /locus_tag="PLW08_00700"
     CDS             complement(9900..10427)
                     /locus_tag="PLW08_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPL51158.1"
                     /translation="MDNKKNNELITEYGNFAYGIANLYRNKGLDWEDLKQEAILGLLK
                     AGEHYDPDKNVQFTTYATYWIKKQILAALKSNSFSYTEEIKENIGADNLPDKITDTNS
                     SNYEPAYVPISPNSIGFDFPEVMPALERTILQLSFGEGKSLKEIAHILQMSVERVTQQ
                     KRKALRRLKSLYPKE"
     gene            complement(10429..11763)
                     /locus_tag="PLW08_00705"
     CDS             complement(10429..11763)
                     /locus_tag="PLW08_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51159.1"
                     /translation="MAGKKNSIALLLSFSFVLLIGLMLILLLIQIPIWLLLIISLIIA
                     ALLTVNMIRILHQQFEQISQTAVQIANGNHSLRIPELETKEFCALGKDLNFMLEKLDK
                     TIYHLAVHREELRLVLSSIDDILWSQSSEGKLEWANEPFQKLFGNIGKKDNVFYTDII
                     RDPYLLSIIKDCGSSKDKLMKEIVMDNHNYLLSASHNDQAKRCIFILQNIDEIKQAEQ
                     MKKDFIVNLAHELRTPLTAISGFAEAMEDNINETNTRYLKIIQNHTQRLIHLISDLEQ
                     LIRLERTAAIELQVINLATFFANIAFIISPMVEEKGLYLKIELDEDLPYLTCDPFKLE
                     QVFINLVQNSLRYTITGGITIRSQKLKNEALFEVCDTGTGIDNIHLPRIFERFYVADP
                     ARNKTQSGTGLGLAIVKHIVQLHQGSITVQSELGKGSVFSVFLPLLQAPETH"
     gene            complement(11744..12460)
                     /locus_tag="PLW08_00710"
     CDS             complement(11744..12460)
                     /locus_tag="PLW08_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPL51160.1"
                     /translation="MDAKGKKIFVVEDETDILELIRMKLESAGFVTKGFKTALPMLNL
                     LKIEHPDLIILDLMLPDLDGMEVCLRIKKDKTTSAIPILMLTARTDLEDKIKGLEYGA
                     DDYVTKPFEGRELIARIKAILRRSEWENGKNALAITENFIIDFNHYEVWIEGKRIDLT
                     LTEFKILQLLTKRPGWVYNRSQILDYLWGNDKIVIERTIDVHIRNLREKLGAYAYLIR
                     NIRGVGYQFLCEEENGRKEE"
     gene            complement(12718..14067)
                     /gene="gdhA"
                     /locus_tag="PLW08_00715"
     CDS             complement(12718..14067)
                     /gene="gdhA"
                     /locus_tag="PLW08_00715"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPL51161.1"
                     /translation="MEELVTINEFMAKVEAKNPGEPEFLQAVKEVVETIWDVYINNPR
                     FVKANILERIVEPERVIIFRVPWVDDKGDVQVNRGYRVQFNSAIGPYKGGLRFHPSVN
                     LSILKFLGFEQTFKNSLTTLPMGGGKGGSDFDARGKSDGEIMRFCQSFMTELFRHIGP
                     NTDVPAGDIGVGAREIGYMFGMYKKLMNEFTGVFTGKGLNWGGSLVRPEATGYGVVYF
                     TEEMLKTKGETIKGKRVAISGFGNVAWGAVQKVNHLGGKVVTISGPDGYILDEEGISG
                     EKINYMVELRASNNDRVKDYADKYPNAKFFPGKRPWEVKVDVAIPCATQNELNGDDAR
                     ALINNNVFCVTEAANMPCTPEAMEIFLKAKILYAPGKAANAGGVATSGLEMTQNSIRM
                     NWSREEVDEKLHGIMKNIHQACVDSGKEPDGYINYVKGANIAGFLKVANAMCDQGIV"
     gene            complement(14343..15563)
                     /locus_tag="PLW08_00720"
     CDS             complement(14343..15563)
                     /locus_tag="PLW08_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPL51162.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGVTLEARKFPQVTRVYGALY
                     GVQGIVNEEFVDLTQETTHNLEQVANTPSSALLSTRDKPDEKYCMEIFKVLKAHDVRY
                     FFYIGGNDSADTVRIVNEQATQADYEFRAIHIPKTIDNDLVLNDHTPGFGSAARFVAS
                     AFTGVNLDNRALPGVYIGVVMGRHAGFLTASSVMAQKYPDDGPHLVYLPEHPFHRENF
                     LNDVKRIYDLYGRCVVAVSEGIVDENGVPIVTKLSGNQEKDAHGNVQLSGTGVLGDLL
                     SELVRTKLKIKRVRSDTFGYLQRSFIGCVSDVDQREAREVGERAAHYAIWYNLDGSIS
                     IQRTGFYSVNYQLENLSDIAGKTRTMPDEYINAEGNGITDEFKYYVRPLLGSGFPVAH
                     RLRAPKVPKLLEDK"
     gene            15707..17308
                     /gene="dnaX"
                     /locus_tag="PLW08_00725"
     CDS             15707..17308
                     /gene="dnaX"
                     /locus_tag="PLW08_00725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPL51163.1"
                     /translation="MSYIVLARKYRPQNFKEVYSQEHITEILQSAIATNRIAHAYLFT
                     GPRGVGKTSLARILAKSLNCVNGPTTTPCNACSNCLEITAGTSPDVIEIDGASNTSVD
                     DIRELQRELLYPASSSNYKIYIIDEVHMLSKNAFNALLKTLEEPPDNVIFIFATTEPQ
                     KVLPTIISRCQRYDFKRIPIASIVQRLQEISILEGIKIDSESLYLIARKADGGMRDAL
                     SLMDQAISYCLNNITIDKVRQIFGMIPNQVYQKFLTLIYNKDTQGLINELHLIFEQGI
                     DLQEFIANMQEFVRILILRKLQVQIKDINPDELSLFDELAELFGQNKLLYILSYLIAC
                     RNDLRGSSNPYLILEALMIKLCKLDEMEDIASLIGKININAFAQPVSMASPTTAKTIQ
                     SSYVASSAEVPYHEESTIAKMEFNQENLEQLWQRIITRAKKISQMCALALDATTDRKV
                     EGRKLCLTVESNTNFRCLKSNQEKLEESISELFEKPISISLQLQETEPPTKVNIQHKT
                     LEDIKHENPELAKFIDLTDSVLVKS"
     gene            17308..18411
                     /locus_tag="PLW08_00730"
     CDS             17308..18411
                     /locus_tag="PLW08_00730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51164.1"
                     /translation="MKKCFPLFTILYSTFLIWLKRGVDKIIAEDKKKSVPDIFQSISI
                     IIAARNEIKNLPNLLNSLACLDYPQDKYEIIIVNDHSNDGSKEYLATQKNIPNLKIIN
                     FYRETPPLTGKKAALQQGIEQAQYEILAFTDADCMVPVYWLQEINRSFTKETDYLLSY
                     SVIKRKPDDSIIRLKNFERSVNYALAAAGLYYKIPFTSSACNMVYRKKTFLESGGFEG
                     IGHLRSGDDDLLLIKMMPNIRKAAYNPNTAMQVTSIDGTDRKKHHQTNIRRASKFKYY
                     PWWLKGLCAFIFIYFCLFYIALWQAIHKKSGKAIKLSLLLKTAAEFAFSYSHLRQIGR
                     GKLAFLYPLQIFVFPLHFIFYGLRGTLGKYTWK"
     gene            complement(18412..18660)
                     /locus_tag="PLW08_00735"
     CDS             complement(18412..18660)
                     /locus_tag="PLW08_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51165.1"
                     /translation="MVYKIVLVKIDQRSTEATKVQGILTKYGCNIRVRLGLHEVSNEF
                     CANDGLVVLEVQGEQNILENMISELNALEYVQARLIEM"
     gene            complement(18673..20382)
                     /locus_tag="PLW08_00740"
     CDS             complement(18673..20382)
                     /locus_tag="PLW08_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPL51166.1"
                     /translation="MDTKENSTENKEEKAVTSNFIRSIIERDLASGKHKSIVTRFPPE
                     PNGYLHIGHAKSICLNFGLAKDYRGRCHLRFDDSNPVKEDVEYVNSIMEDVHWLGFDW
                     GEHLYYASDYFDKLYEYAEYLIKAGKAFVCDLSLEELKEYRGTLTVPGKDSPYRNRSI
                     EENLDLFRRMKAGEFPEGSRTLRAKIDMASPNLNMRDPVIYRIKKTHHHRTGNDWQIY
                     PMYDYTHCVSDALEGITHSICTLEFEDHRPLYDWFLDQLPVPCHPQQIEFARLNLAYT
                     IMSKRLLLELVNNHLVSGWDDPRMPTIAGMRRRGFTPEAIREFSERIGVSKANSLVDD
                     ELLMFCVREDLNKRAERRMVVVNPIKLTITNYPEGKIEEFDAENNPEDPSAGKRKIKF
                     SRNLHIEKDDFSENPVKGWFRLALGKEVRLKYAYYITCNEVIKNEKGEIIELLCTYDE
                     KSRGGWTEDGRKVKGTLHWVCAEDAIPVELRLYDRLFTLADMSNMEEGKTYQDYLNPN
                     SLIVKQNCLAEENLQEAKIGEHYQFLRIGYFNVDYSSKPEALIFNRIVDLKDSWAKLS
                     KKI"
     gene            complement(20629..21366)
                     /locus_tag="PLW08_00745"
     CDS             complement(20629..21366)
                     /locus_tag="PLW08_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPL51167.1"
                     /translation="MNNNETELLFPNLYNNILPFRELIIHISKISKVIHQQGWAEANA
                     GNISIRISDLLMPFIRTNGWENYYPYQDWFLVSRTGSRFRDMIDEPEDCLMIIGIDKE
                     EHYFPDTAKPTSEWLCHRKLQEINKNTNVSCLLHTHPTEVIALSHTSVFQNNKKYPQR
                     LNEYLFEVLPEIQLFLPQGIAVVDYAPPGTLELAEKSCESIQDKQALIWEKHGLLVLA
                     ENIDIALDLTEVINKAAKLLLIKGRKN"
     gene            complement(21524..22162)
                     /locus_tag="PLW08_00750"
     CDS             complement(21524..22162)
                     /locus_tag="PLW08_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51168.1"
                     /translation="MSGFMATFGLWLSVFFTLSIFSFLYKDNPFYKLSEQIFVGLSAA
                     YWLVYLYYSVMVPNLFNKLFTDFKGNLILLIPTALGLMMLLRLIPKTQWLSRYPIAIM
                     IGTSAGINMVRYAKTDLLGQISATMINPFASDNVVTVIGNILLIVGTICGVYFFYFSK
                     KQEGIRAVPSKLGIWFLMISFGASFGYTVMARISLLIGRLEFLLKDWLHIIK"
     gene            complement(22159..22989)
                     /locus_tag="PLW08_00755"
     CDS             complement(22159..22989)
                     /locus_tag="PLW08_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51169.1"
                     /translation="MSANKNTQTERRIIYLVLLVAVALPLIFPIGWKTEVSSYTKMAW
                     ELIENTPDSSVVLFSFDYDPSTITEIQPMAEAMLEHSFSRNQKIIAVALWPQGVQMAD
                     QAFTVALKKYPEKKYGVDYVNLGYKVAGIVAIQAMGKSMQEVFPSDQSGTKYENIPML
                     KKVKTLKDVAYICSLSSGTPGIKEWVMAARDPLGTPVTGGTTAVSAPGLLPYVNSQKQ
                     LYGLLGGLKAASEYETLINKIGSATIKMDAQSIAHLLILVFIIIGNIKAFYSRKRGAQ
                     "
     gene            complement(22986..23684)
                     /locus_tag="PLW08_00760"
     CDS             complement(22986..23684)
                     /locus_tag="PLW08_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51170.1"
                     /translation="MKYKIPLVFAFVCGILVVISEFIPHRPFNMLITTLQDWFMIISG
                     FAILLGQISLFQANIKKIKEKNKDWPFYLAGLISFTVMLIFGLLWGTQEYRGILGNGE
                     KIQATVGMKPFDYLFANAFMPLSSTMFSLLAFFIASAAYRAFIIRSFESNLLMITAVI
                     VMLGRTSFGGMLTSWLPEGLKFLHLPNLSDFIMEFPNSAAQRAILISAALGIIGSSLR
                     IILGIERSYLGGEK"
     gene            complement(23681..24781)
                     /locus_tag="PLW08_00765"
     CDS             complement(23681..24781)
                     /locus_tag="PLW08_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51171.1"
                     /translation="MKKVILISGILFFSTLLFAQSTFFWIEDVPWDDGKALNIKANVP
                     ADTVYLQKALEDGTWETIYTGKGRSLSFTQNELNPDFKYRYRLLVPRPAFNDTIFFMA
                     EAQPKEQWFYLHKLSYLLILLAVSIAIIYYVQNARKGKDYYIRRISGLDCIEDSIGRA
                     TEKGKPILFVPGIMDLDDIQTISSLTILSHLAHKVAEYDTEIIVPCRFSMVLSAAREV
                     VKEAYIKAEKPDAYNQDRIFYLTDDQFGYVAGIDGIMVREQPAANFFLGSFYAESLIL
                     AETGFSTGAIQTAGTAQASQLPFFVVSCDYTLIGEELFAASAYLSRDPQQLGSLKGHD
                     FGKLLVILLIIAGVITEMSGWHWFKAFFEGAQ"
     gene            complement(24973..25770)
                     /locus_tag="PLW08_00770"
     CDS             complement(24973..25770)
                     /locus_tag="PLW08_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51172.1"
                     /translation="MKKTVWLIIAIVILMGLVGCKKKEEKLPSQETASAITTYAPLPS
                     FKEVFRVLDQLQAKDISAAVPADLYKTKQEEVRNAFSLGLLTADATLAAKGHNKKRLS
                     QISSKMMDLTTLLGLEEEVNQMSAELKNLLEKEQWTELETALDNHKKKVEDKLWEMES
                     FDNYTLMLLGGWIEATNRVAWLIKQDYSAEKTKALNQKGTFNSLIGNLKNIKAEHITS
                     QPEFQEVLGLMEQLKAVIDADKEKTYTPEQLDQIISLTNQIKETFKK"
     gene            25999..26181
                     /locus_tag="PLW08_00775"
     CDS             25999..26181
                     /locus_tag="PLW08_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51173.1"
                     /translation="MPSAFQTNRITWAKKNRCYLTVIPIEFLLQYMIKERRNNVNYYF
                     YSLSSNSITEALLTHY"
     gene            complement(26375..27055)
                     /locus_tag="PLW08_00780"
     CDS             complement(26375..27055)
                     /locus_tag="PLW08_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPL51174.1"
                     /translation="MKFQYLGHSAFLFSDSQNRTILIDPFLTGNPLSPVKASQVKADY
                     IILTHAHSDHIGDALTIADKRKTTIICVVELASIISKNGYNTHPLQIGGAFQFPFGKV
                     KMVTAFHGSSTSNGLYAGLAAGVVFKLDHKTIYHCGDTGIFGDMKLIGEIDKIDVLLI
                     PIGGNYTMDIDDAVRAVSLIQPELAIPMHYNTFDLIRANPQEFVDKITANGFAGKVLK
                     PGEELQFS"
     gene            27346..27693
                     /locus_tag="PLW08_00785"
     CDS             27346..27693
                     /locus_tag="PLW08_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPL51175.1"
                     /translation="MTLRDIVELLEAEVLYEGADLNREVPCAFASDLISDILMCTKEP
                     TLLLTGLTNNQVIRLADMIDLIGIVFVRGKKPMQDVIDMAKERGLPLVATNMTLYRSS
                     GILYNAGLRSCRI"
     gene            27665..28183
                     /locus_tag="PLW08_00790"
     CDS             27665..28183
                     /locus_tag="PLW08_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51176.1"
                     /translation="MQDSAVAEFEMSEYWYIKPNFEEKVRDFFAQKREPEVVLELDIP
                     GKDFNIAGKGSSELKNLLKRLGVDSEILRRIAVAGYEAEINVAAHSQGGKMISNVFDN
                     LIYMQFMDNGPGISNIQQAMTPGFSTADDLVRELGFGAGLGLPNIQKNSDALHITSAL
                     NSPTLVEIIIFF"
     gene            28196..29473
                     /locus_tag="PLW08_00795"
     CDS             28196..29473
                     /locus_tag="PLW08_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPL51177.1"
                     /translation="MTETKYFHALQILADNCTGCTACVRVCPTEAIRVRDHKANIDPY
                     RCVDCGNCVNVCRFHAIIPLSDPLEIIHKFKYKLAIISSSFFGQFTEDISYQVAKQAV
                     LNLGFDQVAEEAAVTDFMINIIRDYIRKNRDKRPILSSNCPSVVRLIQLKYPSLLPNL
                     FHQEAPMSILTRYFRDKINKEYGLEEKEIGIFLIVPCIAHVTAVHQPEGAYKHLQDGA
                     FSIGMIYGKIRDSIKELQNNPPSIETYPQGLTWALSGVHAELVDCEDIRTLSVNGVEN
                     VMEILSRVEDHYLDQYDYIVLRTCTNGCVGGCLNVENPFVAMSRIKKMIKEGQGSDFD
                     TSELYELYQKGEFAVVPLAPRPIMELDKDIKKAIQKMKQINEILTMLPGLDCSACGSP
                     TCYALAEDIVLGKASIDDCVVLLRRHSKDSEEE"
     gene            29496..29837
                     /locus_tag="PLW08_00800"
     CDS             29496..29837
                     /locus_tag="PLW08_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51178.1"
                     /translation="MIKLSDYVTAINGINCTPSLALEGVEINGAYVCDMLSDVMASAK
                     SGQVWITIMKHLNVIAVASMTGIPAIIFAKGNEPETAVIEKAIEEGILLISSKLPTFE
                     LAGILYQLLYS"
     gene            29849..30586
                     /locus_tag="PLW08_00805"
     CDS             29849..30586
                     /locus_tag="PLW08_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPL51179.1"
                     /translation="MQSLRADLHIHSVLSPCGGLEMSPNSIMQRLKQFGIQWLAITDH
                     NSMANCPAYQKVAEKEGLFFTWGVEIQSVEEIHLLAYFDDSSSAEKFNAELYESLFPL
                     DNDPDFFGDQVIIDENENILRVEPRALINSSEWNLNTVVEKVQAYNGLVVPAHIDSSV
                     NSILSQLGFMPEVPQFQLFGISACLDVKIWVQDNPYFKDKVFLRASDAHYLNDIGKGY
                     SIITVEKPSVQELFLAAKGCGRRKIEI"
     gene            30617..31099
                     /locus_tag="PLW08_00810"
     CDS             30617..31099
                     /locus_tag="PLW08_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPL51180.1"
                     /translation="MIPADQMQNDQYAHLKKVIEEKKKIRNPLIEILRSAQEIFGYLP
                     VEVQEFIAQELNIPVNQIYGVVTFYNFFTMTPRGKYNLNVCLGTACFVKGAPRLVQML
                     SEELGIQMGETTKDGIFTMSAVRCVGACSLAPVFVIGEETYGRIDSKDKIAEILKRYK
                     "
     gene            31113..31682
                     /locus_tag="PLW08_00815"
     CDS             31113..31682
                     /locus_tag="PLW08_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51181.1"
                     /translation="MQDISLHLLDIIENSVRAKAKNIKIRIIRDVQDNILRLIVEDDG
                     VGMDSQTLAKAQSPFYTSKEEREKKVGLGIPLVKQNAEATNGSFKMTSEPGFGTVLTV
                     DFQLDHIDRMPLGNLKDTLLGAIIGHPEVDFTIKLISHERGVEKSFYFDTAAIKEELG
                     NIPLTYPDVIEYIDQSLLEGIQNTNMEDV"
     gene            31682..32047
                     /locus_tag="PLW08_00820"
     CDS             31682..32047
                     /locus_tag="PLW08_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPL51182.1"
                     /translation="MKTLADLKKIREKAQEEMKLRGGNTRIKIVVGMGTSGIAMGARE
                     VMKTFLEEIANRNLTDVLVTQTGEKGLSSLEPVVDIIEEGKPKVTYGNMTPEKVKKVV
                     VEHIVNGRIVSDYVVATGN"
     gene            32063..33922
                     /locus_tag="PLW08_00825"
     CDS             32063..33922
                     /locus_tag="PLW08_00825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPL51183.1"
                     /translation="MSLKRIDLLICCGSGCVSAGALKVKEQFHNVLKTKGLTNEINII
                     ETGCMGPCDYGPVIVIYPEGVFYKKVTPDDVEEIVNEHFLKGRPVKRLMLQDEEDKTF
                     LTQKEIPFYQKQVKVALANCGYIDPESIEEYIATGGYEALGTVLTTLTPQQTIDIIKK
                     SGLRGRGGGGFPTYLKWQMVHDNPSEEKYFICNGDEGDPGAFMDRSLLEGDPHRVLEG
                     MMIAAYAIGAKNGYFYIRAEYPLAIKRIKMAIEKAKETGLLGKNIFGSEFSFEAEVRT
                     GAGAFVCGEETALIQSIEGQRGNPTPKPPYPAVQGLYKKPTVVNNVETLGNIATIILK
                     GADWFASMGTENSKGTKVFALTGDIRNTGLVEVPMGITLRELIFDVGGGMIGDHKFKS
                     VQLGGPSGGCLTTQHLDVPIDYESLKERGAMMGSGGVIVMNEEKCMVNVAKFFMDFCV
                     DESCGKCSPCKIGLKQMLEILKRIDSGYGKEGDIEELQHLGETISKISLCGLGQTAPN
                     PVLSTIRYFRDEYEAHIRDKSCANKVCPDLMHFEIDKDKCIGCSLCARKCPVSCISGS
                     REEKYTINQLPCIKCGTCMDVCPVKAISKIPGMHPEVKAMREVEELQKQNQEE"
     gene            33935..34390
                     /gene="nuoE"
                     /locus_tag="PLW08_00830"
     CDS             33935..34390
                     /gene="nuoE"
                     /locus_tag="PLW08_00830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPL51184.1"
                     /translation="MIKEICQKYAPRKDNLIQILHEIQDQDPQHYISPEAVDTVAEYL
                     DIPVNHIYGVLTFYTMYSTKPRGKNIIRLCESPPCYIKGSDNMLRKLKVLLGINIGET
                     TKDGLFTLEFTSCLGVCGNAPVMMINDDVYGDLTEEKVEEIIERIRGRR"
     gene            34390..35952
                     /gene="nuoF"
                     /locus_tag="PLW08_00835"
     CDS             34390..35952
                     /gene="nuoF"
                     /locus_tag="PLW08_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPL51185.1"
                     /translation="MKFYRSHILVSINETSLAAGVQEFITALRNELAKNDLAEEINIL
                     ETGPLGFFGRGICLTVYPENINYEGVKIEDIPELVQEHFLKGRPVKRLMVGVTEKFSP
                     KFNYENRIVLRNSGIIDPENIDDYIGAGGYVALEKALTNMQPNDIIAEVKKSGLKGRG
                     GAAFPAGLKWSFTAGLNVPQKYVVVNADEGEPGTFKDRLIMEGDPHQLLEGIILCARA
                     VGASKAYIYIRGEYKLCIARLEKAIKQAYDYGILGKNIFDSGFDLDIELKIGAGAYVC
                     GEETALIESLEGNRGNPRWKPPFPGVEGLWKAPTIVNNVETLANVPFIIAKGADEFLQ
                     YGTPDCPGTKVYTILGDVAYPGLCEVDMGTTLRTIINDYAGGMKKGFRFKAALVGGAA
                     GVILSDRLLDVKMDFTSLNQYSAVLGSGAILVLNEHQSIVDLLWSILRFFRHESCGKC
                     SPCKNGTQQLYHLISKIRKGNGTMEDVNLMLLIAETMQQTSFCALGQSPIMAVRSAIE
                     NFTDEFIEITKK"
     gene            35974..37758
                     /locus_tag="PLW08_00840"
     CDS             35974..37758
                     /locus_tag="PLW08_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPL51186.1"
                     /translation="MAKTVNVFIDGHHLEVPETMTIIEAADKIGIYIPRLCYHPDLPP
                     TGNCGVCVVEIAGNPTPKRACCTPVAEGMKIVTNSKKLRSYRKTVVEMILSNHDVTCP
                     TCPANNKCELQSLANNLGVDSEALPNILVKTPADESSLSIIRDTNKCIGCGRCITVCN
                     EVQTVYALTFAERGIDTHIDTAFSQGMANSPCVNCGQCTVYCPTGALHERSELDAVWE
                     AILDPEKHVIVQEAPSIRVSLGEEFGMPLGSVTPKKMYAALRKIGFDSVMDTNFTADL
                     TIMEEGTECVTKLKAGEKRPLITSCSPGWIKFMETYYPDLADCVSTAKSPMSMFGVLS
                     KTYYAQEKGIDPAKIVSVAIMPCTAKKFEARRPELSDSGFQDTDYVLTTREFIRMIKE
                     AGIDFANLPDEEPDEGMSFYTGAGTIFGATGGVMEAAIRSAYYLVTGTDLQNVDITAV
                     RGLEGVKEASVDVPGFGKVNVAVAHGLSNARKVLDRVREGLATKGESPWHFIEIMACP
                     GGCVGGGGQPYGNDIASRARRGLALYEEDRNLPVRMSHHNPEVLKIYEKFLGEPNSKL
                     ALKLLHTHYFKRSVFNGQVTAEITHKHG"
     gene            complement(38275..39102)
                     /locus_tag="PLW08_00845"
     CDS             complement(38275..39102)
                     /locus_tag="PLW08_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPL51187.1"
                     /translation="MDNLAYFEKLLILLQEHSSVWQANIIESDGSTPAKTGMKLAIPL
                     KEEPFGNLGGGELEHTVIEKIRNDKPGKATIYIFNLSADGSKSGIETSMLCGGYVKVY
                     VEPLFSPDKLYIIGAGHCGKALGKLACLCGFYVVLIDNRKEIIDNLPDDCCHRAIHHD
                     YANLAEIIDFNPHTWIVIMTQGHLHDKEVLEQCLRKPFRYLGMIGSKTKAAATFTKLK
                     EKGFTPEELAKCHSPVGLPIGSNTPYEIAVSIMAELISLRNLQDKEEKSFVHKEIKR"
     gene            complement(39095..39904)
                     /locus_tag="PLW08_00850"
     CDS             complement(39095..39904)
                     /locus_tag="PLW08_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPL51188.1"
                     /translation="MKISAHYSETKFKSFTFEEQIGALNKLILSIEKNLTEAEKRSQI
                     IEHILELYLLVEKPLPQKMQLFIKELNPHLSPHQLLNKLYFYCDGAIRKDSQMALQTE
                     KGKVILDPDIRQKAKQITVICDNLRSVFNVGSLFRTAECLGIGKIIVCGITPTPEHSN
                     MPKTAMGTEKLVAWQYFPSTTEAIAFCHQEGFFIYALETIDNAKSVFQTVYQFPLAIV
                     IGNEALGIESSILELCDEFITLPQLGWKNSLNVGVATGIVLYQILFGGFNG"
     gene            complement(39901..40491)
                     /locus_tag="PLW08_00855"
     CDS             complement(39901..40491)
                     /locus_tag="PLW08_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPL51189.1"
                     /translation="MTAKDIKRAIFLDRDGVISPDDFGYLSNPEEYHLYPYTIEALKI
                     FKELNFLLFVVTNQSGIARGYFTIDDLEKVLAKMRSLLSAGGIELDGVYYSPYYKDGI
                     VPPYNIDHIDRKPGIGMFKKAYSEFHFQIEGSYMIGDRISDLGFGRNAHLKNILLLTG
                     NGEKDFMKILETDDLRPDFVCENLLTAAKLIRDYRL"
     gene            complement(40472..42895)
                     /locus_tag="PLW08_00860"
     CDS             complement(40472..42895)
                     /locus_tag="PLW08_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPL51190.1"
                     /translation="MKNKKICLLLIPLLLLTGLSLFAQNEARNYALSDSLFTEIPDSL
                     KINVIETDSLFYKADSIHFEYDTEIIKLFGKPRINYQDTEIIADSITVDIKKERAFSY
                     GPTKMRDGEQLLLGSDVRYDVKTQTGILNNGYSLIEEGYYTGSELRKVDKDIYDIDGG
                     TFTNCDLEEPSFWFWSKQLRVYRGDKIVGKPVIGYVNHFPAFFFPFIIIPIRQGRHPG
                     FLIPSPDYNNVDGFVIRNLALYYPYRDYADFVLGFDIMEKTGWKGHFDTEYLKRYAFS
                     GNFNAAFQHRIDEYSTFDDWSLQGNHHQDLPEKSSLNATINFVSNKRIWESSIDVDQS
                     LAQRLTSSVSYSKPIGSSYLNAGSYFTQDLINDTASLSLPSASFFLSSRPVSELFNVS
                     SNSWLSNFNYRYNVYLEHTGNLREKNYSLGDFFWDNTIDPADTIGIRMLNEHHLGIKQ
                     NAGISHNSNLFGWLTLGKNFDYTEAWMDRDRNDDKFARGYSWTASANARFNLYGLRNF
                     KNFPINSIRHIITPNIGYSYRPDTRKNSDLYSFGSIGVSNSQEASNLTFNLDNTWQMK
                     YGKKGNETKLNDLLFWRLGTNANLKKKDKPFSNIAHTLYFKPGSFNLGTLSLNEGKYK
                     LGSLKLSYSSQFSASQDPYKIQWDKMNLRNQYFSQGIVLSGSAPYNRYFSAPKNKSFE
                     DFENSDSQKSVLDYMQETAGTNDWSFSISQNIYADKDIFHPATSSLRSSLTCKITQNW
                     RLSYTNYYNLKTNDMLSQTLSFSRDLHCWKMDVNITLRNDYWDYRISLFNTLLPDALR
                     FQTHDSKRY"
     gene            43053..43823
                     /locus_tag="PLW08_00865"
     CDS             43053..43823
                     /locus_tag="PLW08_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPL51191.1"
                     /translation="MKLLLWSWNVNGLRAALNKGFIEIIQKEQPDILGIQETKLQEHQ
                     IPEELNTLNEYLIYWSHSLRKGYSGTGLFTKLLPLSFSTGFGNPEFDNEGRINIAEYT
                     DFIYFNIYFPNGQKDDERLNYKLRFYDRCLEVMEEKRTSGKIILVGGDFNTAHHPIDL
                     ANPKENEKTSGFLPIERAWLDKIAEMGWIDTFRYFDKSPEQYTWWSYRTKARPRNVGW
                     RLDYFWVNKEGIEHIKKAGIRQDIEGSDHCPVFVELEI"
     gene            complement(44487..45269)
                     /locus_tag="PLW08_00870"
     CDS             complement(44487..45269)
                     /locus_tag="PLW08_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPL51192.1"
                     /translation="MRKSFFLILLLLFACTQSSKTISVTEFEHRIWELTNQQRSAYNL
                     PPLLYDEGLADLARLHSKNMFNFDFFAHKDHLGYLVDDRKKKYYPQLVVSSIGENLAK
                     FVNGARTFTPEEVVNGWMNSPEHKENILNATYTHLGVGVFTQDEVLLATQNFAAPVVK
                     LLSEIPEKFDPLYQYQLLFEYMSPLPANELEAVLNFPDSRFKYYIDDRSYTIGLKPVP
                     LHWMAEKQLEVIVDFPAGRGNYSLCFGFNHSYLEDGIKIKVK"
     gene            complement(45285..45734)
                     /locus_tag="PLW08_00875"
     CDS             complement(45285..45734)
                     /locus_tag="PLW08_00875"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPL51193.1"
                     /translation="MKRFSVVLLLILLCSSILAQPIKKNPNKAALCSIFPGGGQIYNE
                     AYIKASAIIGIQTYLIITAIHNDAKVQDYKDKINSTSDEILLAEYRNKQKQYKEKRTR
                     DFWWMGITLAFSTLDAYIDAHLSNFKEEKEKIHIRFEEETLLLEYNF"
     gene            complement(45896..45983)
                     /locus_tag="PLW08_00880"
     tRNA            complement(45896..45983)
                     /locus_tag="PLW08_00880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45947..45949),aa:Leu,seq:aag)
     gene            complement(46088..46426)
                     /gene="secG"
                     /locus_tag="PLW08_00885"
     CDS             complement(46088..46426)
                     /gene="secG"
                     /locus_tag="PLW08_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPL51194.1"
                     /translation="MVLYTIALIIHVIICVALVLVILAQTSKGGLDSNLGGAAMNVFG
                     GSGASKFLKKWTQILAVVFAASCLFLAFLVKNINAGALADIQSRQKKIKDTEQTAPSS
                     KPTTKPATTK"
     gene            46631..46984
                     /locus_tag="PLW08_00890"
     CDS             46631..46984
                     /locus_tag="PLW08_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51195.1"
                     /translation="MARKHAAILQELEIDEVEGYTEAQKRYYTSLGFKPYLNEDGKVK
                     WLRPDQHSLRINAKLKRPFVARLFSHKKVQFPRRRRHRPALVKFVQRNWLFIIIVTIV
                     IVAVVYIIRNPQLIF"
     gene            46998..47768
                     /gene="surE"
                     /locus_tag="PLW08_00895"
     CDS             46998..47768
                     /gene="surE"
                     /locus_tag="PLW08_00895"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPL51196.1"
                     /translation="MKILLVNDDGILAPGIRALAEELKNAGHKITIVAPDSERSAASH
                     SLSIRKELIVKKIAENEYAVSGTPVDCSVIALQKILTEPVDLIISGINAGQNMGEDVL
                     YSGTVGAAMEAALLGNRAIAVSINSYKNQRFEVAAYWMRKLLDLGIDKQAEAFGVLNI
                     NIPNLPLEEIKGIRLTRTGHRKYYNFVKIIKENEDGFVYEVGGDIPVWDNETGTDAEA
                     VNNGYISLTPLGFELTRSETFPRILNWLEENELLQLER"
     gene            47771..48421
                     /locus_tag="PLW08_00900"
     CDS             47771..48421
                     /locus_tag="PLW08_00900"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPL51197.1"
                     /translation="MRFEDQRIRLVNELKLEGITSPVVLNAFAKVPREHYVLPEYRDY
                     AYQNRPLPIKEGQTISQPLMIAIMLQLLDLQPTDIVLEIGTGSGYQSALLAEIVKEVC
                     TVERLETLSLNAQKILRNAGYNNIYFRIGDGYSGWQKAYPPHKEFDKILVSAAAREVP
                     KHLVEQLKTGGKMAIPIGKTGFQILYIVTKTEDGLDIAEAGGCSFVPLVCDDKTLR"
     gene            48425..48910
                     /locus_tag="PLW08_00905"
     CDS             48425..48910
                     /locus_tag="PLW08_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPL51198.1"
                     /translation="MLKDVFDWLKTIWKKPDKYADLKKFPVLSELSSFELYLLNNFLH
                     KRNFNSGELLYDKDHPLEVIYFIFSGEVEVTGPTYPKGRYVVRELQILGLIDMFKEET
                     RSSSATALTEVTAYAVSRYDLSDLFRQNPNLGVKMLTAFCQTLSNYIFNISSPESAKE
                     L"
     gene            48907..49968
                     /locus_tag="PLW08_00910"
     CDS             48907..49968
                     /locus_tag="PLW08_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPL51199.1"
                     /translation="MNWTKWIFYSLLLALIVLGLFFYHPIIYYLIYAFIFSYILDPFV
                     SWFERKRFPRWLAVLTIYIIIAGIIALFTSRLVPALVQQGNNLFNILSSGEQLTGQYI
                     ITLPFIRRIYEFLQELDAQIPGLGMAPKLIEILDQTNVFLAKIPKLLIDNYKKIFSTI
                     SIIFTVPLISFFLLKDKYKLRKGIVKMFSNRYFELCIIMMRKIDSTIGNFLRAILFEI
                     IAVGILASTALTIVGVPYSILIGATAGVANIIPYFGPFLGGALAVVTVLFNGGTILSA
                     ITVILAMYIVQLIDNNIVYPVVVGGTINMHPLLVLLTLIAGGWYGGILWMLISVPLVY
                     LTYNLIKVLYTNLKGFKII"
     gene            49980..51158
                     /locus_tag="PLW08_00915"
     CDS             49980..51158
                     /locus_tag="PLW08_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPL51200.1"
                     /translation="MSLLDKCYKFTDARKVMAMGYYPYFREITSEQDTEVMCNGKKML
                     MLGSNSYLGLTNHPKVKEAAIKAIQKYGSGCAGSRFLNGTLDIHLELENELAKLVGKE
                     EAICYPTGYQANLGCISTMVGKNEYIITDKYDHASIIDGAILAEGKMLRYKHNDMASL
                     ERILKEIKGRNSLIVVDGIFSMEGDIANLPVIIELAEKYNAEIMVDEAHSLGVLGNKG
                     AGTTAHFGLIDKTAFIMGTFSKSLASVGGFIAADEAIIHYLKHKSRALIFSASLPPAS
                     TASVLAALKIMQEEPERIAKLWENTKYMLKEFQAMGYNTGTSCTPVVPLHIGNMMTTF
                     NMWRRLGEEGVFINPVVPPAVPPSSCLIRTSFMATHTKDQLDMALEKFRMIGKELNII
                     "
     gene            51223..52953
                     /locus_tag="PLW08_00920"
     CDS             51223..52953
                     /locus_tag="PLW08_00920"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPL51201.1"
                     /translation="MASKSSVEYYSNIKEMSPIRAIAETLMNNHLVRKVDIREAYELA
                     KNQPGVSVTDLPMYPEFVKLHNLPADAKVINDCHGNIVGRTAKARRFYHRLSPSLKNK
                     LESDFREAVWQMQHYPLIKAEAVLGMDKDLMLKATFVTTESDAANLFFWLLNFAPYES
                     LKEEYEASPKLPIQDIILVAFNEWTCDDDFYNNVGAPQLALVDEKHNVMVNLGMRYFG
                     ERKKGTLTMAWTSGIRIGMAACHGGIKEIDFSTCVNPEYHKLGKRSIAFYGLSGTGKS
                     SHTNNADNAGTLPEGFSKVVLHDDAFQIDLENKLCRVWEPTLFDKTDSRTVDHPDWKY
                     TLALMNHTVLDVNGKLIPYGQDIRNSNGRALFDRSLLGKYVNRCAFPKALVWLMKDSI
                     LPPALKLEDKYLAIAMGAALMTQRNRAENVAEEELNKLVFEPFANPFRVYELYRDVEA
                     FLNVADTGADFYCFNSRGYWRESDDILEPIPLTTSLTLQTAILMDQLQWEPWDLLPGS
                     MIPTRESVEAILPGYYDRYHSNLRINYDKYIAVMKDRFQQRRDFLLNSDLKERPEIQE
                     KLIQALQINA"
     gene            complement(53360..>53595)
                     /locus_tag="PLW08_00925"
     CDS             complement(53360..>53595)
                     /locus_tag="PLW08_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPL51202.1"
                     /translation="KKCSATDMNWVSLPAPENKFRAKAKIRFLHTPAECVVTPINDRK
                     IDVIFDEPQLSITPGQGLVLYAEDLLLGGGFIE"
BASE COUNT        16772 a         9762 c        10237 g        16824 t
ORIGIN      
        1 gctcctacaa tttgcactat tttcatatat aaatccttat ctatgtataa ctaatgtagc
       61 tttaaactga atatgatgta atccatatag attcctgctt cctctgcaat aacaggttat
      121 ctgtatatct taatcctctt ctgcgcctta ataattaaaa ggtttttact gtcaagtagg
      181 aaaggatttt gctttagtat ggttaatgct gtaaagcgta atgaaaggaa cgataaaata
      241 atcctttgcc gaagaaagaa accgattaac ttgttaagcg atggttaaat gaaatggatt
      301 tttaatgctc aggcaggaat aagaaaaaaa tatagtaaac tatgctgtta tcggatagag
      361 aaaaaataaa aatagaagga gcaaataaaa gtgaagaaat tattaatgat tgcagtgttg
      421 ttattcgtta cctgtttaag ccaagggcaa acacagagca gtaaaactaa ggaagaaagc
      481 agaactaccg gtttgggttt tcatttcgga accgtctccg ggaatggatt tagttatcgt
      541 aaatattata ataataagag tggcttacaa gttactttag ggggatttac ctccggctct
      601 aaagaaattg atagctcaat ttatggcaat tataatggtg cttctacgat cactattact
      661 gaaaatggaa aaaaaacaaa gttaagttct gcaatcaatt atgtgcggac attagatacc
      721 aatcaagtag gtaggtttta tattgttgcc ggtggctcac tgctcagttc tttcatcagg
      781 caatatgaaa ttgattatca aatagtaaat aaggcatatt atacaattat aaaagaccat
      841 cccgaaagaa aaaaatggga tagtgaatat gcatattatt ttggtttcgg tttaggtttt
      901 gattttaata tggggcagaa ttttcattgt gcagtggaat tacctctcac aattgacgag
      961 aatagtgaaa ttagaatgta tattccccaa gcagggattt actacttttt taaataatat
     1021 cattttcaga gagaaaattc ttttgttaac ttgaataagc aggaatccaa agataaaaaa
     1081 gcagttaaaa aaagacatat caaaaatgat gaagcccgta agttactttc gggcttcctc
     1141 taattatctg taatccttgt gttaattgta tcttataaac gcttaatcgg ctataagttt
     1201 tttattctcc gattattttt accaagaccc ttttatcgcg cataccgtca aattcgccgt
     1261 agaatatttg ttcccaggtt ccgaaatcca attctccgtt ggttatggca accacaactt
     1321 ctctgcccat aatttgtctt ttcaaatgag catcggcatt atcttcaaaa ccgttatgac
     1381 gatattggga gtagggtttt tctggagcta atttttccag ccaggtctca aaatcactat
     1441 ggagaccttt ttcgtcatca ttgataaaaa cggaagaagt aatgtgcata gaatttacta
     1501 aacacaagcc atcttttatt ccgctttcgt gtacgacatt tctcaccaga ggagttatgt
     1561 ttataaattc cctgcgttta tcggttctga aatgcagttc tcggcgataa cttttcatgg
     1621 acaaaggggt tgagaatcca aataagtgga catctctgta gggctgtcaa aaatcaaatc
     1681 ggctccagca gcttggaggt cttttttgtt tccataaccc caagcagcgc ctatagcaat
     1741 cattcctgca tttttagcgg tctgaatatc tacaacacta tctccaacaa gagcaacatt
     1801 ttgcggtttt acaattaagc gttgggcaag ttccagagct acggtgggat caggtttttt
     1861 gggtttattg ggttcttcac cgctgtaaac gccaaaagga tttttagtat ggcgaatcat
     1921 tgctccccgg aagaaatgac gaatcatttt tttcgtaaaa taatgtggct tgttggaaag
     1981 aattgccagt ttcattttac gggctacagc taattgaatg agatacaata caccggggaa
     2041 aatattggta tgcagatacc attcaatatc gtaatgatcc cagaattttt tagcaagagc
     2101 ttcaatattt tccggggttc ttttatcttc cggtaaaact tttcttgcca attccatatg
     2161 tccatcaccg ataaaggttt tatatgcctc taccggatgt tcagggtagc caagttcttt
     2221 taaggcagca ttcattgcac ctgcgatatc aacaacgctg tcaattaaag tgccatcaag
     2281 atcaaagatg atggctttga tttgaggttt tgtagtcatt cttcacttcc ttactcttta
     2341 ttttatttta agatgtggat tcatttttta gttcctcagc attggcaatg aatatgtgag
     2401 ggaagggaat tgtgatattt tcctgtttaa atttctcctt gataccaatt atcaaagagg
     2461 tcttcaaatt ttgaaaatct tcttttttag tccaaacgcc aaaaagaatc tggattcccg
     2521 agtcaccaaa tttgtctata aaaaacaagg gagctgggtc ttttaatgcc agaggattgc
     2581 tttctgcaag ctctagaaga acttccttaa ctttatgcag atcatcatca tagctaacag
     2641 aaaggtttat atcaacacgg cgcacaggat agcgtgtaat attgataact tccgtcttaa
     2701 tcattgtttc attaggaact cgcacatagc ggttatcagg tgttttcaac ttgatagata
     2761 aaaggtctat ggattcaatt gttccgatat tggaacttac ctgaaccaca tctcctactt
     2821 taaatggctt ttccgatatt aaaaatatgc cactaataat gttggaaaag ctggtctgag
     2881 aagcaaaacc gatagctaca ccaaaaatgc ccgccgctcc taaaatggca gaaagccgaa
     2941 atccaaattc attcagtaag gtaataaaca gaattaaaat tgctacataa taaacggagc
     3001 ggacaattaa ctgttcactt tggggggaaa ggcggttttt aactatcttc ttagtaatgg
     3061 aacgaatcaa tcttaccaaa ggaattccaa ccaccagaat aagaataacc cgaattatca
     3121 aacttatcct ttccggagta ataaaatctc ctgttagctt ttctacatta atagccagta
     3181 gcactttttc tcctcactct aataattttg gcatcctaaa acaagataat cattttggag
     3241 atttatccaa atattatttt actttgaaac acttatcttc tgttaacttt ttgtggttca
     3301 agttttctaa aatagagcaa acacccaata actaccatct ttaattaaat aaccacctaa
     3361 tctctatact tgtaacaatt cagtacttat atacggttta atattatgac aaacaaatta
     3421 tattccgtaa cttattgtta tttaatcaaa ctgaacttta ggggctttct gtgcactttc
     3481 ttctgcctgg aagaattgga agggcttaac cttaataata ctggcaataa tattattcgg
     3541 gaaagttact atcagctgat tgaacttttt agctgcatca ttataacgca ttctttcagt
     3601 tctgattctg ttttcaatac cttccagctg gctttgaaaa gccagaaaat tctggttggc
     3661 tttcaattca ggatagcgtt ccactacaac cattaaacgg gaaagggcat cactcaaacc
     3721 tgcttggctt tgttgaaatg cctgaaatgc ctggggattg gttaatgcgt caggaggaag
     3781 atttaaactt ccaccggctt ttgcacgagc ttcagttact tgagttaaag ttgatttttc
     3841 gaaatttgca gctccttgaa ctatttccac taaatttgga acaagatcat atctggtttg
     3901 ataaacattt tgtacctgag cccaagcact ttctacattc accttttctt tttgaatgtt
     3961 attataacgc ccgataaacc aaccggcaca aattataatc acgaggataa ttgcgagtaa
     4021 tacaatcagt cctttcttca ttttatctcc aaaatttatt ttggttcctt tttaaatata
     4081 tggtttattt tgtccagtaa ttttttctct ccctttcgga gcattgaaag cacaaagaat
     4141 aacagcataa taagggagaa aagtaaaagt gagatttttg aaccgaaagg agaattaata
     4201 taagggctaa acctgtttgt agttggtctt tcgcatagat tatgcgatga agatagtggc
     4261 tttaatacag aaaagataac tgccgctcag cttgttacaa gttatataat cccatttagt
     4321 tatctaataa aacaaagata ggcgggagta aatgaatttt aactttagac ctaaatgtaa
     4381 agaaaactta actttcagct tataaaatct taaccaaaga aaagtataat caaattagag
     4441 gcgaaagcca agaaaaagaa aaaaggagaa taaaatgaaa aaatcccagt tttgcctgaa
     4501 ccttatttgt ttaggattat tgcttatccc ggtaggacta aacggtttag aaaccaaatt
     4561 aagcggagaa ttttgggttc gttggatttc ggaaattgct aaaagaaagg atagcaacgg
     4621 aaattacgat gacaaattag tgagtaacta tctttcattg gaaagaggtt atctgggttt
     4681 ggaaacaaaa tttaccgaac aggtaaaagg tcgttttact gttgatattt ttacaactga
     4741 taagacctac gaatatcagg tatattcttt aagtgacggc acacttactt cttctacaaa
     4801 aagtggaagc atagacggag ctgggcttaa aattaaatat gcttatgttg acttcggtgg
     4861 ttttattccc gttaaagata tgaccttaac agcaggaatg caaaaagttt atttcggtag
     4921 tatttatgac tggagttata ctttaattgg caaagcgccg tctgatgaac ataaagtagt
     4981 aaattcttcc gatttgggta taaccttgaa tggctttttc ccttccggtt ttggagaata
     5041 tcaattgggg atttataacg gagaagggta taagaaaata ggagcaaatc taaaggaaaa
     5101 taccgatttt gcttatttgg gaaatttacg cctaactcca atagccggtt taaccgttgg
     5161 tggttcttat atgcttaata cggtaggacg cgataaaaat cttgatggag aaacggttaa
     5221 tacttcttat gaagaacaga aattaacgga tatatttacg cgcttggctt atggaccggt
     5281 tgatttgaac tgtgagtata ttgttaaaaa tgtaaatttc cccaatatta ctgacagcgg
     5341 taaaacaaaa gagtattccg ctaccggcat ctctatattt ccctgtatta atttgaaagc
     5401 atatttgcct tatgatcttc aattagttgg ttgttatgat gtttgggatg aatcagaccg
     5461 tctggaaaca gataaagcca gaagtaaact taatgctttc acagtcggta taaattataa
     5521 ctttcttccc gatgaaacag acaatcctgc tatgcaatta cagctgaatt ataccgataa
     5581 gaagtatgac gaagataaat ctcatagtga ttatgcagac gggaaaaagg actcatcgca
     5641 aatctatttg cagctgaaat ggaaatttgc caatacacta taaagaaaag gagataaaaa
     5701 tgaaaacaaa aaactatctg atcagcataa taataatgtt acttgcagca ttcaatttaa
     5761 atgcaggaaa gggtaatcaa ataacctgca ccggttctac aacagttctt cctattgccc
     5821 aagcgacggc tgaggcattt atgaattctc atccggatat caatatttct gtgcgaggag
     5881 gaggttcagg agtaggagtt gctgccttac aaaatggaac tacagatatc tgtaattcca
     5941 gccgtccctt aaaatcaaaa gaaatttcgg cagctaaagc aaagggaatt aatcccgttt
     6001 cttacgctat tgccaatgat gcaatcagca ttatagtgca taaggacaat ccccttaagg
     6061 atattagcat tcagcagata aaagatattt acactggtaa gatcaagaac tggaaagtat
     6121 tgggaggtcc caatttaccg attgtagtca tttctcgcga tgtttcttcc ggaacttatg
     6181 aggtctttaa tgaaaaagtt ctagcaggtg cgaaagtaga aggtagtgct caattgcttg
     6241 cctccaataa tgctgtcttg agttcagtaa gtaatactcc aggggctatc ggttatgtgg
     6301 gtttgggata tgtgaataat agcgtaaaac tgatcagcgt aaatggaatc atcccttccg
     6361 aagcaacagt aaagaatggc agttatatgc tttcgcgaaa actctatatg tatactaacg
     6421 gtaaagcaaa aggggatgta gcaaattacc ttggtttcat tcaaagtgaa aaaggacagc
     6481 aaatagttca agaattagga ttcatcaaca tcaagtaatt gattaagaga acaacaggtg
     6541 agggttgcta tttttccctc acctgatgtt agcagggaat aagagatgag tagatataaa
     6601 gaaaaggcat ttacaacaat tacctatact gcagcgttat tttctattat ggttcttttt
     6661 gctttaatat atggcttatt tagagagggt ttgccgcttt ttaataaggt ctctttaact
     6721 gaatttattt tcagcagtag ctggtatcca acgaatgcag aaccggaatt cggtgcctgg
     6781 acttttatta tcggttcttt ttgtgttacc attggagctt tgataatagg aatccccttg
     6841 ggtttgggct ctgcaatttt tatctcagaa atagcgggaa attggttaaa agaaatagtt
     6901 aagcccgtaa ttgaactttt agcaggaata ccttcagttg tttatgggct ttttggaatg
     6961 tccgcaatag cgcctctgat tagaaattgg ttgaatttag atacgggact taatatattt
     7021 actgcttcct tcattttggg gttgatgatt gttcccataa ttgccagtat gagtgaagat
     7081 gctcttacca atgttcccaa atctataagg gaagcggctt tagctttggg ttccaccaaa
     7141 atggaatgta ttttccgagt tattattccc gccgcgcgaa gagggatagt tggcagtatt
     7201 ttgcttggtt tgggtagagc cattggagaa acgatggtgg ttttaatggt ggcaggaggc
     7261 agtgctcaaa ttcctaaaag catttttgat tcagtaagac ccttaacttc tacaatagca
     7321 gcagaaatgg gggagacggt tatgggcagt ttacattatc aatcactcta tgctttggcg
     7381 atattgcttt ttgtgctcac ttttgtaatt aacctgatta ccgaattttt tctcgctaaa
     7441 agggatacaa aatgaagaac agaaaggtct tgaattattg cggcaatggt cttcttttct
     7501 tattggttgg gattactatt ttggttttat gtatcttttt agttagagtt ttttctttag
     7561 gtggcaaggt tttaagctgg gaatttttat ttacagctcc ccgaaactgt atgcaggaag
     7621 gaggtatttt tcctgcattg gtagggactt tttggcttac cgtaatttcc attggtatag
     7681 taattccttt cgggatttta acagctatat ttttaactaa ttatggtaaa ccaaagtggc
     7741 tgttgaaagt ggttcagata gccattgata cattggctgg aactccttcc attatttttg
     7801 gcctttttgg aatggctatt tttgtcaact tttttgcaat gaatgtttcg ctaatttccg
     7861 gagctttaac tttagccatc ctggcattac ctttatttat caattcctgt atagaagcca
     7921 ttaaaagtgt tccttttgat ttttatgaag catctttagc tttgggtgca acgaaaaggc
     7981 aaacaatcac gaaaattgtt ttacctactg ccttgcccaa tattttaaca ggagttatcg
     8041 tttgtattgg gagagttgca ggagaaacgg ctcctattat gtttactgca gctacttttt
     8101 atacgcggcg tttacctaag ggtttaaaag atgaagttat ggctttacct tatcatattt
     8161 atgctttaat gacggaagga actcatccgg aaacacaggt gcccattgct tacgggacag
     8221 cagttgtttt attattgctg gttttaggtg ttagcgccgc ggcagttatt attcgttcat
     8281 ctatcaggag aaaaagaaaa tggtaaataa cattctatca actgaaaatt taaacctatg
     8341 gtttggtagg aactatgtgc ttaaatatgt taaccttaat gtattagaca agaatattac
     8401 tgccgtgata ggaccttccg gctgcgggaa atctacttta ctgcgctgtt ttaaccggat
     8461 gaatgatctt atacaggata cccatattga aggaaaagta aagcttttag atgatgatat
     8521 ttatacttcc aaaatggatg taactgaaat tcgctccaga attggaatgg tttttcaaaa
     8581 acctaatccc tttcctaaga gcatttacaa aaatgttgcc tatggtttac agattcaagg
     8641 taaatatcat aaagacgaaa ttaatgagag agttgaacaa gcattgaaag ctgcttggct
     8701 ttgggaggaa gttaaagacc gtttgcatag tccggctatg gctctttccg gaggacagca
     8761 acagcgttta tgtattgcca gagcattggc aaatagacct gaagttcttt tgctggatga
     8821 accgacttcc gctttagacc ctcagtcaac ctcaaaaatt gaagaactgt gtttggaatt
     8881 gaaaaaaagt gtttgcattt taattgtaac tcataatatt gctcaagcgg gtagaatttc
     8941 caattatacc gcttttatgt atttgggaga attagtggaa ttcggaccca cggaaaaagt
     9001 ttttaccgtg ccagcagata aaagaaccga atcctattta acgggtattt tcgggtaatt
     9061 gctaaaataa ggagaaaata tgcttataga aagaatctcg gaactaaaac aattattgat
     9121 ggcagaagcg ggattggtgg aaaaaatgat tcttttggct atggatggac ttgccaattt
     9181 tggaaccgct tttctggaag aagtgaatac ttttgaaacg agagtaaatc aaattgaaat
     9241 tgaattggac gagaaatgta tgacggtgat tgcgttatat cagccggaag caaaagacct
     9301 gcgtcaaatt ctgatgattt ataaaataaa taacgatttg gaacgcttgg gcgatcaagc
     9361 agtaaatatt gctgaaagtt cggctcaatt ggtgggaagc ccagttttac aattgattcc
     9421 cgaactcttc gcgatgaaag aagcagcttt aaaaatgctg aaagatgccc tttttgcttt
     9481 ctcgggtgag aactatgaac tttcacgcca ggtttgcgat gccgataata ttgttgatga
     9541 gtataatcgt aacatttaca agcatctggt aaagctaatc cgagaaaatc cattacaaac
     9601 cgacctttat ctgcatatct tgcgaatagc taaaaatttg gaaagaattg gcgatttatc
     9661 tactaatatt gctgaagata caatttttct gtcccgaggt aaagtaatta aacataggat
     9721 agacaacata ccttgatagt gtaaagataa aaaatttcag ctcggtaact tattgttcct
     9781 tggttatttg tttaacgact ctttaacact tctttaacac ttctttaaca cttccttaac
     9841 ttcgttaaag aatcgtttaa taatggttaa atgaacatta aagttagcag aaggagagtt
     9901 tactccttag gatacaagct tttcaggcga cgcaaggctt ttcttttttg ctgtgtaacg
     9961 cgttcaacgc tcatctgcaa aatgtgggca atttccttca aagattttcc ttccccgaaa
    10021 gatagctgaa gaattgttct ttccaaagcc ggcataactt cagggaaatc aaaaccgata
    10081 ctatttgggg aaatgggtac atacgcaggt tcgtaattac tgctatttgt atcggttatt
    10141 ttatccggca aattgtctgc tcctatattt tcctttattt cttcagtata agagaatgaa
    10201 ttgcttttta gcgcagccag tatttgtttt ttaatccagt aagttgcata agtagtgaat
    10261 tgcacatttt tatccggatc ataatgttct ccggctttca gcagacccaa gatggcttcc
    10321 tgtttcaggt cttcccagtc caatcctttg ttacgatata agttagcaat tccgtaagca
    10381 aaattgccat attcggtaat tagttcattg ttctttttgt tatccaaatc aatgggtctc
    10441 tggtgcttgt aaaagcggta agaagacaga aaaaacgctg cctttgccga gttcactctg
    10501 cacggttata ctgccttgat gtaattgaac tatatgtttt acaattgcca agcccaatcc
    10561 tgttccgctt tgggttttat ttctggcggg gtctgccaca taaaagcgtt caaaaatacg
    10621 aggtaaatgg atgttatcta tacctgtgcc ggtatcgcaa acctcaaaaa gggcttcatt
    10681 ttttagcttt tggctgcgga tggtgattcc acccgtgatt gtataacgca aagaattttg
    10741 gactaaattg atgaagacct gttccagttt gaaagggtca caagtgagat atggcaaatc
    10801 ttcatccagc tcaattttca ggtaaaggcc tttctcttcc accatcggac taatgataaa
    10861 ggcaatatta gcgaaaaagg tggctaagtt aattacttga agttcaatgg cagcggttct
    10921 ttccaggcga atcagctgtt ccaaatcgct aattagatga attaggcgct gagtgtgatt
    10981 ttggattatt tttaagtatc ttgtatttgt ctcattgata ttatcttcca tagcttccgc
    11041 aaaaccgctg atagcagtta aaggagttct tagttcgtga gccagattaa cgataaaatc
    11101 ctttttcatc tgttctgctt gctttatttc atctatattt tgaagaatga atatgcagcg
    11161 ttttgcctgg tcattgtgcg aagcggaaag caaataatta tgattatcca taactatttc
    11221 tttcatcagc ttatctttgc tacttccgca atccttgata atagacagca gataggggtc
    11281 tctgattata tcggtataga atacattgtc cttttttcca atattaccaa agagtttttg
    11341 aaagggctca tttgcccatt ccagtttgcc ttcggaactt tgagaccaca aaatatcatc
    11401 aatggaacta agcactaaac gcagttcttc acggtggaca gctaaatggt aaattgtttt
    11461 gtccaatttt tccagcataa aattcaggtc tttgcccaaa gcgcaaaatt cttttgtctc
    11521 caattcggga atgcgtaaag aatggtttcc gttcgcaatt tgcactgcgg tttgcgatat
    11581 ttgctcaaat tgctgatgca ggatgcgaat catattcacc gtaagtaatg cggcaataat
    11641 aagagatata attaataaaa gccaaattgg gatttgaatt aacagcagta tcagcatcaa
    11701 tcctatcaat agaacaaaag agaaagaaag taaaagagct atgctattct tctttccggc
    11761 cattttcttc ctcacagaga aattgataac ccacacctct gatgtttcta atcaggtagg
    11821 cataagcacc cagtttttct cttaaattgc gaatgtgaac atcaatagtg cgttcaatca
    11881 caatcttatc gttcccccaa agataatcca aaatctgact gcggttataa acccacccgg
    11941 gtcttttagt aagtaactgc aggattttaa attccgttaa agtaaggtct atccgcttac
    12001 cttctatcca cacctcatag tgattaaaat ctataataaa attttcagta atggctaacg
    12061 catttttgcc gttttcccac tcactccgtc gtaaaattgc tttaattctg gcaataagtt
    12121 ctctaccttc aaagggtttg gttacataat catcggctcc gtattccaag cccttgattt
    12181 tatcttccag gtccgttctt gctgttaaca tcaaaatagg aattgcggag gttgttttat
    12241 ctttttttat tctcaaacaa acctccatac catctaaatc cggcaacatc aaatccagaa
    12301 taatcaggtc aggatgttca attttaagca aattgagcat tggtagggct gttttaaaac
    12361 cctttgtaac aaatcctgca ctctccagtt tcattctgat gagttccaga atgtctgttt
    12421 catcctcaac tacgaatatc tttttacctt ttgcatccat ataaaacctt taaataatat
    12481 aactgatttt gagcaagtaa aaacagccgg gttttggtta aattttctta acaataagtt
    12541 gctgaggttt ttaaggtctg gacgaaattt ggtttgcaac gatatttatc tccttgtttc
    12601 accttttccc ctttttttac ataatatgct ttccagttta ctcattaacc taaaaaagcc
    12661 cgttacgcaa gaatcgtaac gggctttatg tttatcagag agattagggg tttttcttta
    12721 cactatacct tggtcacaca ttgcattggc aactttcaag aaaccggcaa tattagcacc
    12781 tttcacataa ttaatatagc catcgggttc tttgccacta tccacacatg cctgatgaat
    12841 gtttttcata attccgtgca gtttttcatc cacttcttct ctgctccaat tcatacggat
    12901 ggagttttgc gtcatttcca gtccgcttgt agcaactcca ccggcatttg cagctttgcc
    12961 tggagcataa agaatttttg ctttcaggaa aatttccatt gcttcaggag tacaaggcat
    13021 attagcagct tcggttacac aaaatacatt gttattaata agtgccctgg catcatctcc
    13081 attgagctca ttttgcgtag cacaaggaat agcaacatca actttcactt cccagggacg
    13141 ctttcccggg aaaaatttgg cattaggata cttatcggca taatccttta cgcgatcatt
    13201 attggaagca cgtagttcaa ccatataatt aatcttttca ccacttatgc cttcctcgtc
    13261 taaaatataa ccatcaggac cagaaatagt tactactttt ccacccaagt gattcacttt
    13321 ttgaacagca ccccaggcaa catttccaaa tccggaaatg gcaactcttt tacccttaat
    13381 ggtttcacct ttggttttca gcatttcttc agtgaaataa acaacgccat aacctgtggc
    13441 ttcaggacga actaaacttc caccccaatt aagaccttta cctgtaaaaa ctccagtaaa
    13501 ttcattcatc agctttttat acattccaaa catatagcca atttcacgag ctccaacacc
    13561 tatgtcacca gccggaacat ccgtgttggg accaatatga cgaaatagtt cagtcataaa
    13621 ggactggcaa aaacgcataa tttcgccatc actttttcct ctggcatcaa aatcactgcc
    13681 acctttgcct ccacccatag gaagagtcgt taagctgttt ttgaaggttt gttcaaagcc
    13741 caaaaacttc aaaatggata aatttacact gggatgaaaa cgcaatcccc ctttataagg
    13801 tccgattgca ctgttaaatt gaacacgata gccacgattt acttgaacat cgcctttgtc
    13861 gtcaacccaa ggaacacgga aaataattac gcgttcgggc tctacaatac gctcaaggat
    13921 attggcttta acaaaacggg gattattaat ataaacatcc cagattgttt ctactacttc
    13981 tttaactgcc tgtaagaatt caggttcacc tggattcttg gcttccactt ttgccataaa
    14041 ctcattgatg gtcacgagtt cctccatata ttatattgtt tttcttaacc taaaaataat
    14101 tctatcaatt tatgtcaagt aggaaaaaga aaaggttaac gggataacga gttaacgggt
    14161 gaacaggtga acaggttaac agggaaataa aaatcgttgc aaactctgaa atgggcaatt
    14221 ggagcttgct gatgctctgc atttatgaac aatggcgtct tcttcggttc atctataatc
    14281 gttacaagtc ctcgctgcaa aataaaattc ctttattctt taaaatcctg cttattccag
    14341 tcctatttat cttccagcaa cttaggaact ttgggtgcac gcaaacgatg agctacagga
    14401 aaacctgaac ccaaaagagg tcttacataa tacttgaatt catcggtaat tccatttcct
    14461 tcagcattaa tatattcatc gggcatagtt cttgtttttc cggcaatatc gcttaaattt
    14521 tccaactggt aattcacaga gtaaaatccc gttctttgaa tagaaattga accatccaaa
    14581 ttataccaga tagcataatg agctgctctt tctccaactt cgcgtgcttc cctttgatca
    14641 acatcactta cacagccgat aaaagaacgc tgtaaataac caaaagtatc gctacgcact
    14701 cttttaattt tcagtttagt gcgaacaagt tcactaagta aatcacccaa aactcctgtt
    14761 ccggaaagct gaacattgcc atgagcatct ttttcctgat ttcctgaaag ttttgtaaca
    14821 atgggcactc cattttcatc aacaatacct tcggaaacgg ctactacaca tctgccataa
    14881 agatcataaa tgcgttttac atcatttaag aaattctctc ggtgaaaagg atgttcaggt
    14941 aaatatacaa gatgaggtcc gtcatcagga tatttttgtg ccataactga agaagcagtt
    15001 aaaaaaccgg catgacgacc cataaccact ccaatataaa ccccgggtaa agcgcgatta
    15061 tctaaattga ctcctgtaaa agcagaagct acaaatcgtg ctgcagagcc aaaaccaggt
    15121 gtatgatcat tcaaaaccaa atcgttatca atggttttag gaatatgaat agcccgaaat
    15181 tcataatccg cttgggttgc ctgttcattt acaatgcgaa ctgtatcggc agaatcgttg
    15241 cctccgatat agaaaaaata tctgacatcg tgtgctttta gcactttaaa aatttccata
    15301 caatactttt cgtccggttt atcccgcgta gaaagtaatg ctgaagatgg tgtattagcc
    15361 acctgttcca aattgtgagt tgtctcctgg gttaaatcta caaattcctc attcacaatt
    15421 ccctgaactc catatagagc tccataaaca cgcgtaactt ggggaaattt tcttgcttcc
    15481 agggttactc ctactaagga ctgattgatt acagcagtag gacctccgcc ctgtgctata
    15541 acaacttttc cttccagttt cattttaaat cctccattct gattaagctt cataaaacac
    15601 ttaaaacaaa gtgctgataa tctgtccagt agaaaatact tgcttaaaaa agtgtctgta
    15661 gaaagatggt aaaataaatg tattgtatta tatttcgtga ggataaatga gttacattgt
    15721 cttagccaga aagtaccgcc cccagaactt taaagaagtt tactctcagg agcatataac
    15781 ggaaatcctg caaagtgcca tagctacaaa tagaatagct cacgcttatt tatttacagg
    15841 accccgaggt gtaggaaaaa cctcattggc aagaatttta gcaaagagtt tgaattgtgt
    15901 aaacggacca actactacac cttgtaatgc ctgttccaat tgtttggaaa ttacggcagg
    15961 aacttctccc gatgtaattg aaattgatgg tgcttccaat accagtgtag atgatattcg
    16021 tgaattgcaa cgcgagcttt tatatcctgc cagcagttcc aattataaaa tatacatcat
    16081 tgacgaagtg catatgctct ctaaaaatgc tttcaatgca ttgcttaaga ccctggaaga
    16141 accgccggat aatgttatct tcatttttgc tacaacagaa ccccaaaagg ttttgccaac
    16201 tattatttct cgttgtcagc gttatgattt taaacgtatt ccaattgctt ccatagttca
    16261 gcgattgcag gaaataagca tcctggaggg gataaaaatt gattccgaat ccctttattt
    16321 aatagctcgt aaggcagatg gaggaatgcg cgatgcccta tcgttgatgg atcaggcaat
    16381 ttcatattgt ttgaataata ttactattga caaagtccgt caaatatttg gtatgattcc
    16441 caatcaggtc tatcagaaat ttctaacttt gatttacaat aaagatactc agggtcttat
    16501 taacgaattg catttgattt ttgaacaggg aattgacctc caagaattta tagccaatat
    16561 gcaagaattt gtgaggatcc ttatattacg taaactgcaa gttcaaatta aggatataaa
    16621 tcccgatgaa ctttctttgt ttgatgaatt agctgaatta ttcgggcaga acaaattgct
    16681 gtatattctt tcttacctta ttgcctgtag aaatgattta aggggaagta gcaatcccta
    16741 ccttatttta gaagcactga tgattaaact ttgcaagctt gatgaaatgg aagatattgc
    16801 ctctttaatt ggcaagatta atataaatgc ctttgcccaa cctgtatcta tggcttctcc
    16861 aacaacagct aagacaattc aaagcagtta tgtagccagt tcggctgaag ttccttacca
    16921 cgaagaatca actatagcta aaatggaatt taaccaggaa aatctggaac agctttggca
    16981 gagaattatt acccgcgcta aaaaaatcag tcaaatgtgt gcccttgctt tagatgctac
    17041 tacagacaga aaagtggaag ggcgaaaact ttgtttaaca gtagaaagca atactaattt
    17101 ccgctgtttg aaaagcaatc aggaaaagct ggaagaatct atttccgaac tttttgagaa
    17161 acctatttct atctctttgc aattacagga aacggaacct cctaccaaag ttaatattca
    17221 acacaaaaca ctggaagata taaaacacga aaatccggag ctggcaaaat ttatagacct
    17281 cacagattcc gtgctggtta aaagctaatg aagaaatgct ttcctctatt tacaatattg
    17341 tattctactt ttctaatttg gctgaaacga ggcgtagata agataattgc tgaagacaaa
    17401 aagaaatcag ttcccgatat ttttcaaagt atttccataa ttatcgcagc cagaaatgaa
    17461 ataaagaatc tgccaaattt actaaactcc cttgcctgtt tggattatcc ccaggacaaa
    17521 tatgaaataa ttattgtaaa tgaccatagc aacgatggca gtaaagaata tttggctact
    17581 cagaaaaata ttcctaatct taaaattata aatttctata gggagacccc acccttaaca
    17641 ggaaaaaaag ctgccttgca acaaggtata gaacaagctc aatatgaaat tcttgctttt
    17701 accgatgccg attgtatggt ccccgtttac tggttacaag aaataaaccg gtcttttact
    17761 aaagaaacag attacctgct ttcctattca gttataaaaa gaaaacccga tgacagcatc
    17821 attcgtctga aaaattttga acgcagtgtt aattatgctt tagctgcagc gggcttatat
    17881 tacaaaattc cttttactag cagtgcctgt aatatggttt acaggaaaaa gacctttctg
    17941 gaaagtggtg gttttgaagg aataggacat ttaagatcag gtgatgacga tttactatta
    18001 ataaaaatga tgcctaatat cagaaaagca gcgtacaatc ccaatacagc aatgcaggtt
    18061 acctctattg atggcacaga cagaaaaaag catcatcaaa caaatatccg cagagcttcc
    18121 aaattcaaat attatccctg gtggctgaaa ggtctttgtg cctttatttt tatttatttt
    18181 tgtctgtttt atattgctct gtggcaagct atacataaga aaagcggtaa agcaattaaa
    18241 ctttctttac tgcttaaaac agcagccgaa tttgcttttt cctatagcca tttaaggcaa
    18301 attggaagag gcaaactcgc ttttttgtat cctcttcaga tatttgtatt tccccttcac
    18361 ttcattttct atggcttgcg gggaacttta ggaaaatata cctggaaata gttacatttc
    18421 aattaacctg gcttgcacat attccagagc attaagttct gaaatcatat tctccagaat
    18481 attttgttct ccttgcacct ccagaacaac taaaccatca ttggcgcaga attcattaga
    18541 gacctcatgt aaacctaaac ggacgcgaat attgcatcca tacttggtta aaattccttg
    18601 aacttttgta gcttcagtgc tacgttgatc aatttttaca agcacaatct tgtaaaccat
    18661 aatatcctcc ccttatattt tcttactgag ctttgcccag ctgtctttta agtccacaat
    18721 acggttaaaa atcaatgctt cgggcttact gctataatca acattgaaat aaccaattcg
    18781 cagaaactga taatgttctc caatctttgc ttcttgcaaa ttttcttctg ctaaacagtt
    18841 ttgtttaact atcagcgaat ttggattcaa gtaatcctga taggtctttc cttcttccat
    18901 attactcata tccgctaaag tgaaaaggcg atcatataaa cgcaattcta caggaatagc
    18961 atcttccgca caaacccaat gtaaagttcc tttcactttg cgtccatctt ctgtccatcc
    19021 gccgcgtgat ttttcatcgt aagtgcaaag caattctata atttcgccct tctcattttt
    19081 gattacttca ttacaagtga tataataagc gtattttaag cgaacttctt tgcccaaggc
    19141 aagacgaaac caacctttca ccggattttc actgaaatca tctttttcta tatgaagatt
    19201 gcgggagaac tttattttcc tctttcccgc agaaggatct tccggattat tttccgcatc
    19261 aaactcctct attttgcctt ctgggtaatt agtaatagtt aattttattg gatttaccac
    19321 taccattctg cgctcagctc ttttattcaa atcctcgcgt acacaaaaca tcagtagttc
    19381 atcatctact aaagaattag ctttggaaac accaatccgc tcagaaaatt ccctaattgc
    19441 ttccggagta aaacctcttc tgcgcattcc tgcaattgta ggcatcctgg gatcatccca
    19501 accgcttact aaatggttat ttaccaattc cagtaaaagc cgtttactca ttattgtata
    19561 agcaagattc aaacgggcaa attctatttg ttgcggatga cacggaaccg gtaattgatc
    19621 taaaaaccag tcgtataaag gtcggtgatc ctcaaattcc aaagtgcaaa tagagtgtgt
    19681 tattccttcc aaagcatcgg aaacacaatg tgtgtaatca tacataggat aaatctgcca
    19741 gtcattaccg gttctatggt gatgggtttt tttaattctg taaataaccg gatcgcgcat
    19801 attcaaatta ggcgaagcca tatcaatttt tgcccttaaa gttctgctgc cttcgggaaa
    19861 ttcacctgct ttcatccttc tgaacagatc aaggttttct tctatactgc gatttctgta
    19921 gggactatct ttccccggaa ccgttaaagt gcctctatat tctttcagtt cttccaaaga
    19981 gagatcacaa acgaatgcct tgccggcttt gattaagtat tccgcatatt catacagttt
    20041 atcaaaatag tcagatgcat aatataaatg ttcaccccag tcaaaaccta accaatgcac
    20101 atcttccata atggaattaa catattccac atcttctttc accggattgc tgtcatcaaa
    20161 acgcagatga caacgacccc tatagtcctt tgccaaaccg aaatttaaac agatggattt
    20221 ggcatggccg atatgtaaat aaccattggg ctcaggagga aaacgcgtta caatactttt
    20281 atgctttccg cttgctaaat ccctttctat tatagagcgg ataaaattgg aagttactgc
    20341 cttttcttct ttattttcag tgctattttc ctttgtatcc atattcaatc ctttctttat
    20401 aatcattttg tcatattatg atagtgtgct ttttctgtca agtgcaatga aggttatcct
    20461 ctcacagatt tcacagattg aataaaaata tggataaaaa ataagagcac gcagattacg
    20521 cagataacgc agattacgca gatttttact tataattatt cttatataat ataaaatcca
    20581 gctgatccag ttatctgcgt tctattgttt ttctcctttt aactaacttc agttcttccg
    20641 accttttatt agcagaagtt ttgcggcttt attgattact tccgtaaggt ctaaggcaat
    20701 atctatgttt tcagccagaa cgaggagacc atgcttttcc cagataagtg cttgtttatc
    20761 ttggatggat tcacagcttt tttctgctaa ttctaaagtt ccgggaggag cataatcaac
    20821 cacagcaatt ccttggggaa ggaataattg tatttcagga agtacctcaa ataagtattc
    20881 attcaagcgt tggggatatt tcttattatt ctgaaaaacg gaggtatggg aaagagcgat
    20941 aacttctgtg ggatgggtat gcagaagaca agatacatta gtatttttat taatttcttg
    21001 cagtttacgg tgacagagcc attcggaagt aggtttggca gtatcaggaa agtaatgttc
    21061 ttctttatca ataccgatta tcattaaaca atcctccggt tcatctatca tatcccgaaa
    21121 acggctgcca gttctggaaa caaggaacca atcctgataa ggatagtaat tttcccaacc
    21181 gtttgtcctg ataaatggca tcagcaaatc tgatatacgg atagaaatat ttccggcatt
    21241 agcttcagcc catccttgtt gatggataac tttggaaatt ttactaatat gtatgattag
    21301 ctctctaaaa ggcagtatat tattatagag attggggaat aacagttcag tttcattatt
    21361 gttcattatc taacctacct attatttagt cgttattaat ccttaacatc ttgtactatt
    21421 tattatgcga agacgggaaa tgacgaggat atcatttatt ccggtatcca aatttccctc
    21481 tcgacttctc gccctcaaga cctctcgccc tctcaccatc tcactactta atgatgtgca
    21541 gccaatcctt cagcaaaaat tccagccgtc ctatcaataa agagatacga gccataaccg
    21601 tatagccaaa agaagcacca aaactaatca tcaaaaacca gattcccagt ttggaaggaa
    21661 cagctctgat gccttcttgt tttttactga aatagaagaa ataaacaccg cagatggttc
    21721 ctacaattaa aagtatgttt ccaataactg ttacaacatt atcagaggca aaaggattaa
    21781 tcattgtagc gctgatttgt cccaaaaggt ctgtcttggc atagcgaacc atattaattc
    21841 cggcagaagt gccaatcata attgcaatgg gatagcggct aagccattgc gtttttggaa
    21901 ttaagcgcag caacatcatc aaacccaaag cggtaggaat aagcaaaatc aggtttccct
    21961 taaaatcggt aaagagttta ttaaacagat tgggaaccat aacgctataa tataaataga
    22021 ccaaccagta ggcagcggaa agtcctacaa aaatttgttc ggagagttta tagaaaggat
    22081 tatccttata gagaaagctg aagatgctta aagtgaagaa tactgatagc caaagtccga
    22141 aggtagccat aaatccgctc attgtgcacc cctttttctg ctgtagaaag cttttatatt
    22201 tccgataatg atgaaaacca aaatcagcag atgagcaatg gactgtgcat ccattttgat
    22261 agtggcgctg ccaattttat tgatcagtgt ttcatattct gaggcagctt taagtccgcc
    22321 aagtaagcca taaagttgtt tctggctatt tacataaggt aataaaccag gagcgctaac
    22381 tgctgtagtt ccgcctgtaa caggagttcc taaaggatcg cgagcagcca taacccattc
    22441 tttaattcct ggagttccac tggaaaggga acaaatatag gcaacatctt tcagggtttt
    22501 aacctttttc aacattggaa tattttcata ttttgtcccg ctttgatcgg agggaaaaac
    22561 ctcttgcatt gattttccca ttgcttgaat agcaacaata cctgcaactt tgtagcccaa
    22621 attaacataa tccacaccat attttttctc aggatatttt ttaagggcta cagtaaatgc
    22681 ctggtctgcc atttgcacac cttgaggcca aagagccacg gcaataattt tttgattgcg
    22741 ggaaaaagaa tgttccagca ttgcttctgc cattggttga atttccgtta tcgtagaagg
    22801 atcgtaatca aaggaaaaga ggacaacaga cgaatcggga gtattttcta tcaattccca
    22861 agccattttt gtgtagctgg agacctcagt tttccagccg ataggaaaaa taaggggcag
    22921 agcaactgca actaaaagga ctaaatagat tattctgcgt tcggtttggg tatttttatt
    22981 agcgctcatt tttcgcctcc cagataggat ctttcaatac caagaataat ccgtaaactg
    23041 ctacctatta ttcctaaagc agcgctaatc agaattgccc tttgagctgc agaattggga
    23101 aattccataa taaaatcgct caaattggga agatgtaaaa atttaagccc ttctggaagc
    23161 caggaagtaa gcattccacc aaaactggtt cttcccaaca ttactattac agccgtaatc
    23221 attaatagat tggattcaaa actacgaata ataaaagcac gataagcagc tgaagcaata
    23281 aaaaatgcca gcagggaaaa cattgttgac gatagtggca taaaagcatt ggcaaacaga
    23341 taatcaaagg gtttcattcc taccgttgcc tggatttttt ctccattgcc cagaattcct
    23401 ctatattcct gggttcccca cagcaagcca aaaattaaca ttacagtaaa actaatcaga
    23461 cctgcgaggt aaaatggcca gtccttgttt ttttctttga tttttttaat gtttgcctga
    23521 aacaaactga tttgccctaa aagaatggca aaaccagaaa taatcataaa ccaatcctgc
    23581 aaagtggtaa tcagcatatt gaaaggacgg tggggaataa attcggaaat aactaccaga
    23641 attccacata cgaaggcaaa gacaaggggg attttatact tcattgtgcc ccctcaaaaa
    23701 atgctttaaa ccaatgccag ccagacattt ctgttattac tccagctata attaatagaa
    23761 ttactaaaag tttaccgaaa tcgtgtcctt ttaaagaacc cagctgttgc ggatcacgag
    23821 aaagataagc ggaagcggca aaaagttctt ctccaatcaa agtgtaatca caagaaacca
    23881 caaaaaaggg taattgactt gcctgagcgg ttccagctgt ttgaattgcg cctgtactaa
    23941 aaccggtttc ggctaaaatt aaggattcgg cataaaaact tccgaggaaa aaattggctg
    24001 ctggctgttc gcgtaccata attccatcaa ttccagctac ataaccaaat tggtcatcgg
    24061 ttaagtaaaa aatcctgtcc tgattgtaag catcgggctt ttcagctttg atatatgctt
    24121 cttttacaac ttctctggca gcggataaaa ccatagaaaa cctgcaggga acaattattt
    24181 ccgtatcata ttccgccact ttatgagcca aatggcttaa gatggttaaa ctggaaatgg
    24241 tttgaatatc atccaaatcc ataattccgg gaacaaaaag aatgggtttt cccttttccg
    24301 tagccctgcc aatggaatcc tcaatacaat ccagaccgct aatcctgcga atataatagt
    24361 cctttccttt gcgagcattt tgaacataat agataatggc aatggagaca gcaagcagaa
    24421 ttaaaagata ggaaagcttg tgcagataga accattgttc cttgggttga gcttctgcca
    24481 taaaaaatat ggtatcatta aatgcaggac gtggaaccag caaacggtat ctgtatttaa
    24541 aatcaggatt aagttcattt tgcgtaaagg aaagagacct tcctttacca gtgtaaattg
    24601 tttcccaggt tccatcttcc aaagcttttt gcagatagac agtatcggca ggaacatttg
    24661 ctttaatgtt tagcgccttt ccatcatccc agggaacatc ttcaatccaa aaaaaggtgg
    24721 attgggcaaa taacagggtg gaaaagaata gaatacccga aattagtatt acttttttca
    24781 ttatcttcct tttagttttt tgcaggatga caaacaacaa tttgccacaa agttcaaaaa
    24841 aatccattat tctgtatctg tcccatcaga attcaataga acagccatag ataataaggt
    24901 tgggaaagca atatcagcaa tccttaaatc ttgctttttc ccaacctgat tgtaacataa
    24961 taatagcaga aattactttt taaaggtctc tttaatctga ttagtcagcg aaattatttg
    25021 atccagctgt tccggagtat atgtcttttc tttatcggca tcaataaccg ctttaagctg
    25081 ttccatcaaa cctaaaacct cttgaaattc aggttgactg gtaatatgtt ctgctttgat
    25141 atttttcaaa ttgccgatta agctattgaa tgtgcctttt tgatttaatg ccttggtttt
    25201 ttctgcagag taatcctgtt ttataagcca ggcaacgcga tttgtagctt caatccaacc
    25261 gccgagaagc atcagagtgt agttatcaaa actttccatt tcccagagct tatcttccac
    25321 ttttttcttg tgattatcca aagcggtttc cagttcagtc cattgttctt tttcaagcaa
    25381 atttttcagt tcggcactca tttgatttac ttcttcctcc aaacccagaa gagtggttaa
    25441 atccatcatc ttggaagaaa tctgactaag gcgcttttta ttatgacctt tagcagctaa
    25501 agtagcatct gccgttagta aacctaaaga aaaagcgtta cgaacctctt cttgcttggt
    25561 tttataaaga tcagcaggaa cggcagctga aatgtctttt gcctgaagtt gatccagaac
    25621 tctaaaaacc tctttaaatg aagggagagg ggcataagtt gtgatagcgg atgctgtttc
    25681 ctggctgggt aatttttctt cctttttttt acaaccaacc agacccatca aaattacgat
    25741 ggcaataatt aaccaaaccg tttttttcat tgtttactcc ttttatctaa ttcatcattt
    25801 agcaaaagct tatgtctgat aaaaaagggt tttattaact ttttttcttt gtttaccaag
    25861 gataaagaat tgtcaaggaa aaaaggcaaa ctattcggaa ggttgaagtc atcatcaacc
    25921 aagttaaagc atatttaaca atgcccaaaa ttgtaatgtt aaactccaat aatgtattga
    25981 aaagaaatga aagggaaatt gccttcggcc tttcaaacaa accgcattac ttgggctaaa
    26041 aaaaaccgtt gttatttaac tgtaattcct atagaatttt tactacaata tatgattaaa
    26101 gagaggagaa ataatgttaa ctattatttc tattctcttt catccaatag tattactgaa
    26161 gcattgctta cccattacta atgccatccg taatgggtaa gcaatgggta agcaatgcta
    26221 ttaaagaaga agtgttttta gttagatgta attaaaatgt attaaataaa ttccccattg
    26281 ctaaaagggt tggcagatta ttatacaaaa aactcaattt cttttaccct ctccgaattt
    26341 gtcatcccgt ttttgtattc acgcagtaaa acatttagga aaactggagt tcttctcccg
    26401 gttttaacac ctttcctgca aaaccgttag cagtaatttt atccacgaat tcctgcggat
    26461 tagctctaat taaatcaaaa gtattatagt gcataggaat agccaattcc ggctgaatca
    26521 aactgacggc acgcacagca tcatcaatat ccattgtata attgccacct ataggaatca
    26581 ggagaacatc aattttatct atctctccaa tcaatttcat atctccaaaa atgcctgtat
    26641 cgccacaatg ataaatggtt ttatggtcca atttgaaaac aactcctgca gctaaaccgg
    26701 cataaagacc gttagaggtt gaggaaccat gaaaagcagt aaccattttt actttaccga
    26761 aaggaaattg aaaagcgcct cctatttgca gaggatgagt attatagcca tttttggaaa
    26821 ttatggaagc aagctcaact acacagataa ttgtggtttt tcgcttatct gctatggtta
    26881 atgcatcacc tatatgatcg gaatgagcat gagtaagaat gatataatcc gctttcactt
    26941 gactggcttt tacaggtgac aggggattgc ctgttaaaaa agggtctata aggattgtgc
    27001 gattttggct atctgaaaac agaaaagccg aatgtcccaa atattgaaat ttcatttttc
    27061 tcctcctttt gtttgtgcta aagatataat aattcgtttg ggcacaataa acaacattaa
    27121 aatatagggg gaaacgacaa tagattttgt attaacttac aattttttaa tttatgaacc
    27181 agttatagct tggttgtatg tgtaatagtt ggtggggtaa tgattattgg gagggaaagt
    27241 gaaaattgta agttaaaacc tctctaccct cttacttttc ccttgactga atttcaatat
    27301 ctatatttaa tgaaataaca aaaaaaagat taagggaaaa agtgaatgac cttacgagat
    27361 attgtagaac ttctggaagc ggaagttctt tacgaaggag ctgatttgaa ccgggaagtt
    27421 ccttgtgctt ttgcctcgga tttaatttcc gatattctga tgtgcaccaa ggagccaacc
    27481 ctacttttaa ccggacttac caataatcag gtaatccgtc ttgccgatat gatagattta
    27541 ataggcattg ttttcgttcg tggtaaaaaa ccaatgcaag atgttattga tatggcaaaa
    27601 gaacgaggtc ttccattagt tgcaactaat atgactttat accgttcaag tgggatatta
    27661 tataatgcag gactccgcag ttgccgaatt tgaaatgtcc gaatattggt atataaaacc
    27721 aaattttgaa gaaaaagtgc gggacttttt tgctcaaaaa agagaaccgg aagtagtttt
    27781 ggaacttgat attccaggga aggattttaa tattgctgga aagggttcct cggaattgaa
    27841 gaatttgcta aaacgcttag gagtagattc ggaaatttta agacgcattg cagttgcggg
    27901 ttatgaggca gaaataaatg ttgctgctca ttctcaaggt ggaaaaatga tcagcaatgt
    27961 gtttgataat ctaatttata tgcagtttat ggataacgga cctggaatta gtaatattca
    28021 acaagcaatg acccccggtt tttctacagc cgatgattta gtgcgcgaat taggttttgg
    28081 agccggtttg ggcttaccta atattcaaaa aaacagtgat gcattgcata taacatcggc
    28141 attaaattct cccaccttag tagaaataat aatatttttc taagataaaa gcgatatgac
    28201 tgaaacgaaa tattttcatg ctctgcagat tttagcagat aattgtaccg gctgcactgc
    28261 ttgcgttaga gtttgtccta cagaagcaat aagggttaga gaccacaagg caaatattga
    28321 tccttaccgt tgtgtggatt gtggcaattg tgtaaatgtt tgtcgttttc atgctataat
    28381 tccgcttagc gatcctctgg aaataattca taagttcaaa tacaagctgg ctattatttc
    28441 ttcttctttt tttggccaat ttacggaaga tattagctat caagttgcca aacaggcagt
    28501 tctaaatctc ggttttgatc aagtggcaga agaagcggca gtaacggatt ttatgattaa
    28561 cattattcgt gattatatca gaaaaaatcg ggataaaaga cctattttaa gtagtaattg
    28621 tccatccgtt gtgcgcttaa ttcaactaaa atatccttct ttactgccga atctttttca
    28681 ccaggaagct ccaatgagca ttttaaccag gtattttaga gataagataa acaaagaata
    28741 cggtttggaa gaaaaagaaa taggcatctt tttaattgtt ccttgtattg ctcatgtgac
    28801 tgcagttcac caacctgaag gcgcttataa acatcttcag gatggagctt tttccattgg
    28861 aatgatttat ggcaaaatac gggattccat aaaagaactg caaaataatc ctccttctat
    28921 tgaaacatat ccccaaggtt taacttgggc tctttccgga gtacatgcag aacttgttga
    28981 ttgtgaagat atccgcactt tatccgtaaa cggagtggaa aatgtgatgg aaatcctttc
    29041 ccgggtggaa gatcattacc ttgatcaata tgactatata gtattgcgaa cttgtaccaa
    29101 cggttgtgta ggtggttgtt taaatgtgga aaatcctttc gtagctatga gcagaattaa
    29161 aaaaatgatt aaagaaggtc agggaagcga ttttgatacc agtgaacttt atgaactcta
    29221 tcaaaagggc gaatttgctg tagttccact tgcgcccaga ccaattatgg aactggataa
    29281 ggatatcaaa aaagcaattc agaaaatgaa acagataaat gaaattctca caatgcttcc
    29341 aggacttgat tgcagtgcct gtggaagccc tacctgttat gctctggcag aagatattgt
    29401 tttaggaaag gcatccattg atgattgtgt ggtgctgtta aggcgccata gtaaagatag
    29461 tgaggaagaa taaaatcaaa ataaggatag gataaatgat taagctaagt gattatgtta
    29521 ctgctattaa tggcatcaac tgcactcctt ctcttgccct ggaaggagtg gaaataaacg
    29581 gtgcctatgt ttgtgatatg ctaagcgatg ttatggcttc tgccaaatca ggacaggttt
    29641 ggataacgat tatgaaacat ctgaatgtta ttgctgttgc ttctatgact ggcattccgg
    29701 caataatttt tgctaaagga aatgaacccg aaacagcagt tatagaaaaa gcgattgagg
    29761 aaggcatttt acttatttcc agcaagttac ctacttttga acttgctggt attctttacc
    29821 aattacttta ttcctaagtt attttcggat gcaatcgctc cgcgctgatt tacatattca
    29881 ttctgtttta tctccctgtg ggggtttgga gatgtctccc aattcaatta tgcaaagatt
    29941 aaagcaattt ggcattcaat ggctggcaat tactgatcat aacagtatgg caaattgtcc
    30001 tgcctaccaa aaagtagcgg aaaaagaagg actttttttc acttggggcg tagaaattca
    30061 aagtgtggaa gaaattcatc ttcttgccta ttttgatgat tcttcttccg ctgaaaaatt
    30121 caatgcagaa ctttatgaaa gcttgtttcc gcttgataac gatcccgatt ttttcgggga
    30181 tcaagtaatc attgacgaaa atgagaacat tttaagagtg gaaccacgcg ctcttataaa
    30241 ttcctcagag tggaacttaa acactgtagt ggaaaaggtt caggcatata atggacttgt
    30301 tgttccggct catattgatt ccagtgtaaa tagtatctta agtcagctgg gttttatgcc
    30361 agaagtgcct caatttcagc ttttcgggat cagcgcttgt ctggatgtta aaatctgggt
    30421 gcaggataat ccttatttta aggataaggt ctttttgcgt gcctcggatg ctcattactt
    30481 aaatgatatt ggtaagggct acagcataat aactgtagaa aagccatcgg tgcaggaatt
    30541 gtttttggct gcaaagggtt gtggcaggcg taaaattgaa atttaataaa aaatatagat
    30601 aaaataagga gaaattatga ttcccgcaga tcagatgcaa aatgaccagt atgcacatct
    30661 gaaaaaagtg attgaagaaa agaaaaaaat ccgcaatccc ctcattgaaa ttcttcgttc
    30721 agctcaggaa atattcggtt acctgcctgt ggaagtgcaa gaatttatag ctcaggaatt
    30781 aaacattccc gtcaatcaga tttatggagt agttactttt tacaactttt ttacgatgac
    30841 tcctcgcgga aaatataatc ttaatgtttg tttaggcact gcctgtttcg ttaaaggagc
    30901 tcctcgctta gtccaaatgc tttctgaaga attgggaatc caaatggggg agaccacaaa
    30961 agacggcatt tttacaatga gtgcagttcg ttgtgtaggt gcctgttctt tggctccggt
    31021 atttgtaatc ggggaagaaa cctacggtag aattgatagt aaggataaaa tagccgaaat
    31081 cctgaaacgc tacaaataat ccggattctg aaatgcaaga tatttcatta caccttctgg
    31141 atattataga aaattccgtt cgcgccaaag caaagaacat taaaatccgt attattcgcg
    31201 atgtgcagga taatatcctg cgtttaattg tggaagacga tggagtagga atggattctc
    31261 aaaccttagc taaggcgcaa agtccatttt atacatctaa agaagagcgg gagaaaaaag
    31321 taggactcgg catacccctg gttaaacaaa atgcagaagc aacaaacggc agttttaaaa
    31381 tgaccagtga acctggtttt ggcactgttt taacggtgga ttttcaatta gaccatatag
    31441 acagaatgcc cctgggaaat ctgaaagata ctcttttagg tgctattata ggtcatcccg
    31501 aagttgattt taccattaaa cttatcagcc acgaacgcgg tgtggaaaag agcttttatt
    31561 ttgataccgc cgctatcaaa gaagaattgg gaaatatacc tttaacttat cccgatgtta
    31621 ttgaatacat tgatcaatca ttattagaag gaatacaaaa cacaaatatg gaggatgtct
    31681 gatgaaaaca ctcgcagacc ttaaaaaaat ccgtgaaaaa gctcaggaag aaatgaaact
    31741 tcgcggtgga aacacacgca tcaaaatagt ggtcgggatg ggcacatccg gaatcgctat
    31801 gggcgcacga gaagttatga aaaccttcct ggaagaaatt gctaaccgca atttaactga
    31861 tgttttagtt actcaaacag gtgaaaaagg gctttcttcc ctggaacccg ttgtggatat
    31921 tattgaagaa ggcaagccaa aagttactta cggcaatatg actcccgaaa aagttaaaaa
    31981 ggtagttgta gaacacatag taaatggcag aatcgtttca gattatgttg tcgctacagg
    32041 caactgattc tggaggaaaa gaatgtcatt aaaacgtatt gacctattga tttgctgtgg
    32101 gtcaggatgt gtaagtgccg gtgccttaaa agttaaagaa cagtttcata atgttctgaa
    32161 aacaaaagga ctgacaaatg aaatcaacat cattgaaacc ggttgtatgg gaccctgtga
    32221 ttatggtcca gtaattgtaa tttacccgga aggtgttttc tacaaaaaag ttacccctga
    32281 tgatgtagaa gaaattgtga atgaacactt tttaaagggt cgtcccgtaa agcgtttgat
    32341 gcttcaggat gaagaagata agaccttcct tacgcagaaa gaaatccctt tttaccaaaa
    32401 acaggtgaaa gtagcccttg ccaattgcgg ttatattgat ccggaaagca ttgaagaata
    32461 cattgctacc ggtggttatg aagcattggg aactgtttta actactctaa ctcctcagca
    32521 gaccatagat attattaaaa aatcaggatt gcggggaagg ggaggaggcg gttttcctac
    32581 ttaccttaaa tggcaaatgg tgcacgacaa tccttccgag gaaaaatact ttatttgcaa
    32641 tggagacgaa ggcgatcccg gtgcttttat ggatagaagt ttattggaag gtgatccgca
    32701 tcgtgttttg gaaggaatga tgattgctgc ttatgctatc ggagccaaaa acggatattt
    32761 ttatatccgg gcagaatatc ccttggctat caagcggatc aaaatggcta ttgaaaaagc
    32821 taaggaaacc ggtttgttag gtaaaaacat ctttggcagt gaattcagtt ttgaagctga
    32881 agttagaacg ggtgcaggtg cctttgtttg tggcgaagaa acagctttaa ttcaatccat
    32941 tgaaggtcaa agaggaaatc caactcccaa accaccctat cctgcagtgc aaggtcttta
    33001 taaaaagcca accgtagtaa ataatgtaga gaccttaggc aatattgcaa ctataattct
    33061 aaaaggagct gattggtttg cctctatggg aacagaaaac agtaaaggga caaaggtttt
    33121 tgccttaaca ggagatataa gaaataccgg tttggttgaa gttccaatgg gaattacttt
    33181 acgggaactg atttttgatg taggtggagg aatgattggc gaccacaaat tcaaatcagt
    33241 tcaattagga ggtcccagtg gcggctgttt aactactcag catttggatg ttcctattga
    33301 ttatgaaagc ttaaaagaac gtggagcgat gatgggttcg gggggagtga ttgtaatgaa
    33361 tgaagaaaag tgtatggtga atgttgccaa gttctttatg gatttctgcg tggatgaatc
    33421 ctgtggaaaa tgttctccct gcaagattgg cttgaaacaa atgctggaaa tattaaagag
    33481 gattgacagc ggatatggca aagaagggga tattgaagaa cttcaacacc tgggagaaac
    33541 catttccaag atttcccttt gtggattagg acaaacagct cccaatcctg ttctttccac
    33601 aattcgttat ttccgcgatg aatatgaagc tcatattcgg gataaaagtt gcgccaataa
    33661 ggtttgtccc gatttaatgc attttgagat agacaaggat aaatgcatag gatgttcctt
    33721 atgtgcgcgc aaatgtcctg taagttgcat ttccggcagt cgggaagaaa aatatacgat
    33781 caatcaacta ccctgcatca agtgcggaac ctgtatggat gtatgccctg taaaagccat
    33841 ttccaaaatt ccgggaatgc atcctgaggt taaagcaatg cgtgaagtag aggaattaca
    33901 aaagcaaaat caagaagaat aaaggataag agctatgata aaagaaattt gtcaaaaata
    33961 tgctccccgt aaggataatc tgattcagat tctccatgaa attcaggatc aagacccaca
    34021 gcactatatt tctcctgaag cagtagatac tgttgctgaa tatcttgata ttcctgtgaa
    34081 tcatatttat ggagtgctga cattttacac tatgtatagc actaaaccaa gaggcaaaaa
    34141 tattatccgc ctttgcgaat caccgccctg ttatattaag ggctcggaca atatgctgcg
    34201 gaaactgaaa gttctgcttg gcatcaatat tggcgaaaca acgaaagacg gtttattcac
    34261 acttgaattt acttcctgtt taggtgtttg tggaaatgca cctgtgatga tgattaatga
    34321 tgatgtttac ggtgacctca cggaagagaa agtagaagaa attattgaaa gaatcagagg
    34381 gaggagataa tgaaattcta tcgcagtcat attttggtct caattaatga gacctctctc
    34441 gcagctggag ttcaggaatt tattactgct ctccgcaatg aattagccaa aaatgattta
    34501 gcggaagaaa tcaatattct ggaaacagga cctttgggct ttttcggcag agggatttgc
    34561 ttgactgtat atcccgaaaa catcaattat gaaggcgtta aaatagaaga tataccggaa
    34621 cttgtgcagg aacatttcct gaaaggacgc ccggtaaaac gcttaatggt tggagttaca
    34681 gaaaaattca gtcccaagtt caactatgaa aaccgcattg ttttacgcaa ttcaggtatc
    34741 attgaccccg aaaatattga tgactatatt ggtgctggcg gttatgtagc attggaaaag
    34801 gcattaacta atatgcaacc aaatgatatt attgccgaag tgaaaaaatc gggattgaaa
    34861 ggaagaggtg gagcagcatt tccggcaggt cttaaatgga gctttaccgc aggtcttaat
    34921 gttccccaaa aatatgtagt tgttaatgct gatgaaggag aacccggcac ttttaaagat
    34981 cgtttaatta tggaaggaga cccgcatcaa ttgctggaag gtattatact ttgtgcgcgt
    35041 gcggtaggtg ccagtaaagc atatatctat atccgcggtg aatataagct ctgtattgcc
    35101 cgtttggaaa aagccattaa acaagcttat gactatggca ttttaggcaa aaatatcttt
    35161 gatagcggat ttgacttgga tattgaactc aaaattggag ccggagctta tgtttgtgga
    35221 gaggaaacag ctttaataga atccctggaa ggaaacagag gaaatccgcg ttggaaacca
    35281 ccttttcccg gagtggaagg actttggaaa gctcctacga tagttaataa tgttgagacc
    35341 ttggcaaatg tacctttcat tattgccaag ggtgccgatg aatttttgca atatggaacc
    35401 cctgattgtc ctggaactaa ggtctataca attcttggag atgttgctta tccgggactt
    35461 tgcgaagtgg atatgggaac aactttaaga accattatca acgattatgc aggtggaatg
    35521 aaaaaaggtt tccgtttcaa agcagcattg gtaggtggag cagccggagt tattctttcc
    35581 gatcgccttt tagatgtgaa aatggatttt accagcttga atcaatattc tgctgtttta
    35641 ggtagcggag ccattttagt tttgaatgaa caccagagta ttgttgattt gctttggagt
    35701 attctgcgtt tcttccgcca tgaatcctgc ggaaaatgtt ctccttgtaa aaacggaacc
    35761 caacagcttt atcatctaat atccaaaatc cgcaaaggaa acgggactat ggaagatgtg
    35821 aatttaatgc tcttaatagc tgaaacgatg cagcaaacat ctttctgtgc tttaggccaa
    35881 tcgccaatta tggctgtccg cagtgcgatt gaaaatttta ccgatgaatt cattgaaata
    35941 actaaaaagt aagaaaataa gaggattgat attatggcta aaacagtaaa tgtctttata
    36001 gacggtcatc atctggaagt tccggagaca atgactatta tagaagccgc agataaaatt
    36061 ggaatttata ttccccgttt gtgttatcac cccgatttac ctcctacagg aaattgtggt
    36121 gtttgtgttg tggaaattgc aggaaatcca actcccaaac gtgcctgttg tactcctgtg
    36181 gcggaaggaa tgaaaattgt tactaattca aagaaacttc gttcctatcg taagacagtt
    36241 gtggaaatga ttctttccaa tcatgatgta acttgtccta cttgtcctgc caacaataaa
    36301 tgcgaattac aatccctggc aaataatctg ggagtggatt ctgaggcgtt acccaacatt
    36361 ttggttaaaa cacctgccga tgaatccagt ttgtccatta tcagagatac caataaatgt
    36421 atcggatgcg gacgttgtat aaccgtatgt aacgaagtgc aaacggttta tgctctcact
    36481 tttgctgaaa gaggaattga tacccatatt gatactgcct tcagtcaggg aatggcaaat
    36541 agcccatgtg ttaattgtgg tcagtgtact gtttattgtc ctacgggtgc attgcacgaa
    36601 agaagtgaac tggatgctgt ttgggaagct attttagacc ctgaaaaaca tgtgatagtt
    36661 caggaagcac cttccattcg tgtttcttta ggtgaagaat ttggaatgcc tttaggcagt
    36721 gtaactccta aaaaaatgta tgcagctctg cgtaaaatcg gttttgattc ggttatggat
    36781 accaatttta ctgccgattt aaccattatg gaagaaggca cggaatgcgt aaccaaactg
    36841 aaagcgggag aaaagagacc gctgattact tcctgttctc ccggttggat taaatttatg
    36901 gagacctatt atcccgattt agcggattgt gtttcaacgg caaaatcacc gatgagtatg
    36961 tttggagttc tttccaagac ctattatgcc caggaaaaag gaattgatcc tgccaaaatt
    37021 gtctcggttg ctattatgcc atgcaccgct aagaaatttg aagcacgacg tccggaatta
    37081 agcgatagcg gttttcagga taccgattat gttttaacta cgcgggaatt tattcggatg
    37141 attaaagaag cgggaattga ttttgctaac ctaccggatg aagaaccgga cgaaggaatg
    37201 agtttttata caggtgcagg aacaattttt ggagccacag gcggagttat ggaagctgca
    37261 attcgtagtg cttattacct tgtaactggc acggatttgc agaatgtgga tattacggca
    37321 gtgcgtggat tagaaggagt taaagaagca tcggttgatg ttcccggctt tggaaaagtg
    37381 aatgtagctg ttgctcatgg actttccaac gcgcgtaaag ttcttgatcg tgtgcgcgaa
    37441 ggtcttgcta caaaaggtga atccccctgg cattttattg aaattatggc ttgtccggga
    37501 ggttgtgtag gtggaggagg ccaaccttac ggcaatgata ttgcttccag agcgagaaga
    37561 ggtttggctt tatatgaaga agaccgcaat ttacctgtca gaatgtcgca tcataatccg
    37621 gaagttctaa agatctatga aaaattcctc ggtgaaccaa attccaaact tgcccttaaa
    37681 ctattgcata cgcattactt taaacgctcc gtattcaacg gacaggttac cgcagaaatt
    37741 actcataaac acggataaac tcctactact aaaagataca gaaaggcgga gatttctttc
    37801 tctgccttat tttttttatc agatgataaa aaacacagta taagctcaaa caaggcaatt
    37861 tgttaaaaat gggttggcaa cttcctattt gacaaagagt ttgtagaacc ctgagaaata
    37921 aatgtcttta ttttatgctg aaatatagtt ccagttataa gagtaatata ttgtggggtg
    37981 tagtattcgg gaaggcaggt gaaaattgta agtcaaaact tttccaccgt tattttctct
    38041 ttttctcttt gtgaataaaa ctcttttcct ctttatctct ttatctcttt gtgaaaaaaa
    38101 actcttttcc tctttatctc tttatctctt tgtgaataat acttttttcc tctttatctc
    38161 tttatctctt tgtgaaaaaa aactcttttc ctctttatct ctttatctct ttgtgaaaaa
    38221 aactcttttc ctctttatct ctttatctct ttgtgaaaaa aactcttttc ctctttatct
    38281 ctttatctct ttgtgaacaa aactcttttc ctctttatct tgaagattac gcaacgagat
    38341 aagctctgcc ataatactca ctgcaatttc gtaaggtgta tttgatccga taggtaaacc
    38401 tacaggtgaa tggcatttag ccagttcttc cggtgtaaat cccttttctt tcaatttagt
    38461 aaaagtagcg gctgcttttg ttttactgcc tatcattccc aaatagcgaa agggttttct
    38521 caggcattgt tccagaactt ctttatcgtg caagtgacct tgagtcataa tcacaatcca
    38581 agtatgggga ttaaaatcaa taatctcagc caggtttgca taatcgtgat gtatagcacg
    38641 atgacaacaa tcatcaggta aattatcaat aatttctttt cggttgtcta tcaggacaac
    38701 ataaaaaccg caaaggcaag ctaattttcc taatgctttg ccacaatgtc ccgctccaat
    38761 aatataaagt ttatcggggg aaaataacgg ttcaacatat acttttacat aacctccgca
    38821 cagcatagaa gtttcaattc cggatttaga gccatctgca gataaattaa aaatataaat
    38881 tgtggctttt ccgggtttat catttctaat tttctctata accgtatgtt ccaattcccc
    38941 tccacccaaa ttgccaaatg gttcttcctt taacggaatg gctaatttca ttccagtttt
    39001 tgccggagtt gaaccatcgc tttcaataat atttgcctgc cagacagagc tatgttcttg
    39061 cagtaaaata agcagttttt caaaataagc aaggttatcc attaaaacct ccaaacagaa
    39121 tttgataaag gacaattcct gttgctacgc ctacatttaa agaatttttc caacccaatt
    39181 gaggcaaagt gataaattca tcacaaagct ctaaaattga tgactctatg ccaagagctt
    39241 catttccgat aacaatcgca agaggaaatt gataaacggt ctgaaataca ctttttgcgt
    39301 tatctatcgt ttccagagca taaataaaaa aaccttcttg atggcaaaaa gcaattgctt
    39361 cagtagtgct tggaaaatat tgccaggcaa cgagtttttc cgttcccata gctgtttttg
    39421 gcatattact atgttctgga gtcggggtta ttccgcagac aataattttt cctatcccta
    39481 agcattctgc agtccgaaaa agagaaccaa cattaaaaac cgagcgtaaa ttatcacaaa
    39541 tcacggttat ctgttttgct ttttggcgaa tatcaggatc aagaataact tttccttttt
    39601 ccgtttgaag tgccatctga ctatcttttc tgatagctcc atcacagtaa aaatagagtt
    39661 tatttaataa ttgatgagga cttaagtgag gatttaattc ttttataaag agctgcattt
    39721 tttgaggtaa gggtttttct actaaaagat ataattccaa aatatgctca attatctgag
    39781 agcgtttttc cgcttccgtt aagttctttt ctattgataa gataagttta ttcaatgctc
    39841 ctatctgttc ctcaaaagta aaggacttga atttggtttc gctgtaatga gcggaaattt
    39901 tcataaccta taatccctaa ttagtttagc agcagtcaat aaattctcgc agacgaaatc
    39961 cggtcttaaa tcatccgttt ccagaatctt cataaagtcc ttttctccat tccctgtcaa
    40021 cagtaagata tttttaaggt gagcatttct gccgaaacca agatcactaa tgcggtctcc
    40081 aatcatatag gaaccctcta tttgaaaatg aaactcggaa tatgcctttt tgaacattcc
    40141 aatgcctggt ttgcgatcta tgtgatcaat attatatggt ggaacgatcc cgtctttata
    40201 ataaggggaa taataaacgc catcaagttc tattcctcct gcggaaagca aagaacgcat
    40261 ttttgccagc actttttcca agtcgtcaat agtaaaataa cccctggcaa ttccggactg
    40321 attggtaacc acgaaaagca aaaaatttag ttccttaaat atctttaacg cttcaattgt
    40381 gtaaggataa agatgatatt cttccggatt gcttaaatag ccaaaatcat caggacttat
    40441 aacgccatct cggtctaaaa aaatagcgcg tttaatatct tttgctgtca tgggtttgaa
    40501 accttaaagc gtccggtaac aaagtattaa acagggaaat tcggtaatcc cagtaatcat
    40561 ttctcaaagt gatatttaca tccattttcc agcaatgcag gtctcgggaa aaagaaagtg
    40621 tttgagaaag catatcattt gttttcaggt tataataatt ggtgtaagat aatctccagt
    40681 tttgcgtgat tttacaggtt aagcttgatc tcaaactact ggttgcggga tgaaaaatat
    40741 ctttatcggc atagatattt tgactgatgg aaaaactcca gtcattggtt cctgcagttt
    40801 cttgcatata atccaaaact gacttttgag aatcggaatt ttcaaaatcc tcaaaacttt
    40861 tgtttttagg agcagaaaaa tatcggttgt aaggtgcaga accgctaaga actattccct
    40921 gagaaaaata ttgattcctt aagttcattt tgtcccactg aattttatag ggatcttgag
    40981 atgcgctaaa ctgtgaggaa tagctaagtt ttaaactgcc aagcttatat tttccttcat
    41041 ttaaagatag agttcccagg ttaaaacttc ctggtttgaa atatagtgta tgtgctatgt
    41101 tagaaaaggg tttatccttt tttttcagat tagcatttgt tcccaatcgc caaaataaaa
    41161 ggtcattaag ttttgtttca tttccttttt tgccgtattt catctgccaa gtattatcta
    41221 aattaaaagt tagattggat gcctcttggg aattgcttac cccgatactg ccaaaactgt
    41281 ataaatcgga attcttcctg gtgtccgggc gataggaata gccgatatta ggtgtaatta
    41341 tatgccgaat agagttaata gggaaatttt taaaattccg caaaccgtac aaattaaatc
    41401 tggcatttgc agatgcagtc caggaataac ctctggcaaa tttatcatca tttctatctc
    41461 tatccatcca cgcttccgta tagtcaaaat tcttccctaa agttaaccat ccgaataaat
    41521 tggaattatg agaaattccg gcattttgtt ttattcccaa atgatgttca ttcagcatcc
    41581 taattcctat tgtatcagca ggatcaattg tgttatccca gaaaaaatca cctaaggaat
    41641 aattcttttc tcttaaattc cctgtatgct ctaaataaac attatagcga taattaaaat
    41701 tgcttagcca ggaattggaa gatacattaa aaagttcaga aacgggtcta ctggacaaga
    41761 aaaaagacgc actcggtaaa gataaagaag cggtatcatt aatcaaatcc tgcgtaaaat
    41821 agctgccggc atttaaatat gaagaaccaa tcggtttgga ataggaaaca ctggaagtta
    41881 atctttgagc taaactttgg tctacatcaa tgctgctttc ccaaatgcgt ttattactta
    41941 caaaattgat agttgcattc aaagatgact tttctggaag gtcttgatga tgatttccct
    42001 gtaatgacca atcgtcaaaa gtgctgtatt catctattct gtgttgaaaa gcagcattaa
    42061 aatttccgga aaaagcatat cgtttcaggt attcggtatc aaaatggcct ttccaaccgg
    42121 ttttttccat aatatcaaag cccagaacaa aatcggcata atcacgataa ggataatata
    42181 aagctaaatt ccggatcaca aaaccatcta cattattata atcaggagaa ggaataagaa
    42241 aaccgggatg ccttccctgc ctgataggaa ttataataaa aggaaaaaag aatgccggaa
    42301 agtgatttac ataaccaatt acaggttttc cgacaatttt atctcccctg taaactctta
    42361 attgttttga ccaaaaccaa aaagatggct cttccaaatc acagttggta aaagtgccac
    42421 catctatatc ataaatatcc ttatcaactt tacgaagttc gcttccagtg taataaccct
    42481 cttcaattaa actgtatcca ttattcaaaa taccggtttg agttttaaca tcataacgaa
    42541 catcggaacc taaaagtaat tgttctccat ctctcatttt ggtaggacca tagctgaaag
    42601 cgcgttcttt tttgatgtct acggtgatgg aatcggcaat aatttctgta tcctgataat
    42661 taatgcgggg cttgccaaaa agcttaataa tttccgtatc atattcaaaa tgaatgctat
    42721 ctgccttata aaaaagcgaa tccgtctcta ttacattaat tttcaagcta tcaggaattt
    42781 cggtaaatag ggaatcagac aaagcataat ttcttgcttc gttttgagca aaaagcgaaa
    42841 gacctgttag caacagtaaa ggtatcagta acagacagat tttcttattt ttcatttagt
    42901 aatctaaaaa aataaatgcc atctctgtgt caagcaggat aataacttgc ctgcaggcaa
    42961 taactaaatt attatattag atgaatgcct tttctaaaaa aggaattact taagcataat
    43021 aaccggttga ggttagcaaa aaggagtttt ttttgaaatt attactttgg tcttggaatg
    43081 ttaatggatt acgtgctgct ttgaataagg gttttataga aattatccaa aaagaacagc
    43141 ccgatatttt aggaattcag gaaacaaaat tgcaggaaca tcaaatccct gaagaactta
    43201 acactttaaa tgaatacctt atttattggt cgcatagttt gcgcaaaggg tattcaggaa
    43261 ccggcttatt tacaaaactg ctacctttaa gcttttccac aggatttggc aatccggaat
    43321 ttgataacga aggtagaatt aatattgccg aatatactga ttttatctat tttaatatct
    43381 attttcctaa tggacaaaaa gatgatgaac gcttgaacta taaactccgc ttttatgacc
    43441 gctgcctgga agttatggag gaaaaaagaa cttccggcaa aataatttta gttggcggtg
    43501 atttcaatac tgctcaccat cctatagacc ttgccaatcc aaaagaaaat gaaaagacca
    43561 gcggtttttt acctattgaa cgtgcttggc tggataaaat tgcagaaatg ggttggatag
    43621 atactttccg ctattttgat aaaagccctg aacaatatac ctggtggagc tatagaacaa
    43681 aagccagacc aagaaatgta ggttggcgac tggattattt ttgggtaaat aaagaaggaa
    43741 ttgaacatat caaaaaagct ggaatcagac aagatattga gggttcagac cactgtcccg
    43801 tttttgttga actggaaata taatctatac aacattttta ccaacaagcc ataaataatg
    43861 attatataga cctttgataa aaaataggag gaatacaata gaaaaccgaa agtaagaact
    43921 tgatgccaag attagaagaa attgagcaga caataggtac tttgcctaat ggtttcgtag
    43981 gtcgtagaca tataatagcc gcagcttttt cctgaacgat attacaagac agaagttatg
    44041 ctctcatcat cattttgctt tataaaatta ccttgtggac tttgtttaac tacctgttgc
    44101 ctggtcattt ctttaacgac tctttaacga tcctttaaca atgccttaac ttcgttaaag
    44161 aatcgttaaa gaaggtttaa agaagtataa cagattcaac atggtaggaa ataacgtgct
    44221 gcataaaaaa aaggtaaagg tctcgctctt tctttctttg ccaattcctt ttctctatta
    44281 accttgtttt cccttattgg ctgggagtta tcctgcatta acatttttgg acttctgctt
    44341 ttgccgggat ccacagtgga ctggaaaata tttatgcaat aacgatatga aaactatatt
    44401 cctcaatttg ggtttacctt ttttacctat agacattttc ccttttcacc ttcccacttt
    44461 ttcatttccc ctgttatccc gtttctctat ttaaccttta ttttaattcc atcctccaga
    44521 taggaatgat taaaaccaaa gcagagagaa taattacctc tgccggcagg gaaatctact
    44581 attacttcca attgtttttc cgccatccaa tgcaaaggaa ctggtttcaa gccaatagta
    44641 taagacctgt catctatgta atatttgaac cttgaatcag ggaaatttaa tacggcttcc
    44701 agttcattag caggcaaggg agacatatat tcaaacagca attgatattg atataagggg
    44761 tcaaactttt cgggaatttc ggatagcaac ttcaccaccg gagcggcaaa attctgtgta
    44821 gccaaaagca cttcatcttg cgtaaataca cctactccta aatgagtgta agtggcattt
    44881 aaaatatttt ctttgtgctc aggagaattc atccaaccgt taactacttc ttcaggtgta
    44941 aaagtccttg caccattaac aaatttggct aagttctctc caatagagga aacaactaat
    45001 tgcggataat atttcttttt tcggtcatca acaagatacc cgagatggtc tttatgcgca
    45061 aaaaaatcaa aattgaacat atttttacta tgcaaccggg ctaaatcagc caaaccctca
    45121 tcatacaaaa gcggaggtaa attataagct gaacgttgtt gattggtaag ttcccaaata
    45181 cgatgttcaa attccgttac acttatggtc tttgaagatt gtgtgcaggc aaaaagcagt
    45241 aagagaataa gaaaaaatga tttacgcacc gattttttct ccggctaaaa gttatattct
    45301 aaaagaaggg tttcttcctc aaaacggata tgaattttct ccttctcttc cttaaaattg
    45361 gaaagatgtg catcaatgta agcatcaagg gtagaaaatg ccaaagttat tcccatccac
    45421 caaaaatcgc gagtgcgttt ttccttatat tgcttttgtt tattcctata ttctgcaagt
    45481 aatatttcat cactggtgga attgatttta tccttataat cctgaacttt ggcatcatta
    45541 tgaatagcag taataattaa ataggtctga attccaatta tagcactggc tttaatatat
    45601 gcttcgttgt atatttgccc tcctccaggg aaaatagaac acaaagctgc tttgtttgga
    45661 ttcttcttaa tcggttgtgc caaaattgaa ctgcataaca gaattaagag cagaactacg
    45721 ctaaatcgtt tcaaaccaac tcctgctttt tctttttttc cttatctttt ttccttatta
    45781 aattgtcaat aataatgtaa cgggaggaca tcagagttca tatataaaat aagatacagc
    45841 tgttaaaatg tttactaaaa aaaaggagct tttttaagct ccatctctaa aaagttggtg
    45901 cagaaggcgg gatttgaacc cgcacacctt taacaggcac aagatcctta gtcttgcgtg
    45961 tctgccaatt ccaccacttc tgctttttat attgttttca gaatgaaaag ccccttctta
    46021 ttcctgcgaa agcagtaatc caagcataat ctatcaaaat aactgcattt tcaaaataaa
    46081 agttatatta ttttgtagtt gcaggttttg ttgtcggttt agacgaagga gccgtttgtt
    46141 ctgtatcttt aatctttttt tggcgggatt gaatgtcggc aagggcaccg gcattaatat
    46201 ttttaactaa aaaggcaaga aataaacagg aagcagcaaa aaccactgcc agaatttgtg
    46261 tccatttctt caaaaacttg gaagcaccac ttccgccaaa aacattcatt gctgctccac
    46321 ccaaattaga atccaaacct cctttagagg tctgagctaa tattaccaga accagagcaa
    46381 cacaaattat cacatggata attaaggcaa tcgtgtataa aaccattatt ttatcctttc
    46441 taaacgaatt aatcaatcta ataaaaaatc attgaaaaaa cagccctatt tctgtcaatt
    46501 aaaaagttat gtctactgaa ttcagagctt gtttttccct tattatcact tttttattca
    46561 ggcataatca gattttttgg cttgacagaa atagttgtct aaattgataa tgtaaaaaga
    46621 ggaataattt atggcaagaa aacacgctgc aatactgcaa gaacttgaaa tagatgaagt
    46681 tgagggatat actgaagccc aaaaaaggta ttatacttct ttggggttca agccctatct
    46741 gaatgaagac ggaaaagtta aatggttaag gcctgatcag cattcgttaa ggatcaatgc
    46801 taagttaaaa aggccttttg tagcacgtct tttttctcat aagaaagttc aatttcctcg
    46861 ccgacgaaga catagaccgg ctttagtaaa gtttgttcag cgaaactggc tgtttattat
    46921 tattgtaact attgtcattg tagcggtggt ttatattatt cgtaatcctc agcttatttt
    46981 ttaggagtag aaaaactttg aagatattat tggttaatga tgacggtatt ttagctcccg
    47041 gaataagagc tttagcggaa gagctgaaaa atgccgggca taaaattacg attgtagctc
    47101 cggatagtga aagaagtgca gcatcgcatt ctttgagtat ccgtaaggag ctaatagtaa
    47161 aaaaaatcgc tgaaaatgaa tatgctgtca gcggaacacc tgttgattgt tctgttattg
    47221 cgttacaaaa aatccttacc gagccggtgg atttaattat ttcgggaatt aatgcgggac
    47281 agaatatggg agaagatgtt ttatattccg gaacagttgg agcagcaatg gaagcagctc
    47341 ttttaggaaa tcgagcaata gctgtttcca taaattctta taaaaatcaa agatttgagg
    47401 tagcagctta ctggatgcgt aaattgctgg atttaggcat tgataaacaa gccgaagcgt
    47461 ttggtgtttt gaatattaat attcccaatc ttcctctgga agagattaaa ggtattcgtt
    47521 taacccgtac aggtcatcgt aagtattata attttgttaa gattattaaa gaaaacgaag
    47581 atggttttgt ttacgaagta ggaggtgata ttcctgtttg ggataatgaa acaggaaccg
    47641 atgcagaagc ggttaataac ggttatatat ctttaactcc tttgggcttt gaacttacaa
    47701 gaagcgagac ctttcccagg attcttaact ggctggaaga aaatgaatta ctgcaattgg
    47761 agagatgagg atgcgttttg aggatcaacg gataagattg gtaaacgaat taaagttgga
    47821 aggaataact tctccggttg ttttaaatgc ttttgccaaa gtgccgcgag agcattatgt
    47881 tttacctgaa tatcgtgatt acgcatatca aaaccgtccc ttacctataa aagaaggtca
    47941 aaccatatct caacctttaa tgatcgcaat tatgttgcag ttgcttgatt tgcaacccac
    48001 ggatattgtt ttggaaattg gcaccggtag tggttatcaa agtgccctac ttgcagaaat
    48061 tgttaaagag gtatgtactg tagaacggtt ggagaccctt tccctaaatg cgcagaagat
    48121 tttacgcaat gccggttata acaatattta ttttcggatt ggtgatggtt acagcggttg
    48181 gcaaaaagca tatcctccac ataaggaatt tgataaaata ttggtttctg cagcagcgcg
    48241 ggaagttccc aaacatttag tggaacaatt aaaaacggga ggtaaaatgg caattcccat
    48301 aggtaaaacc ggttttcaaa ttttgtatat agttaccaaa acagaagacg gattggacat
    48361 tgcagaagca ggaggttgtt cttttgtgcc tttggtttgt gatgataaga cattgaggtg
    48421 aaaagtgctt aaagatgtat ttgattggct gaaaacaatc tggaaaaaac cggataaata
    48481 tgccgactta aaaaagtttc ctgttttaag tgaactcagt tcttttgagc tttatttgct
    48541 caataacttt ttgcataagc gcaattttaa cagcggagag ttgctttatg ataaagatca
    48601 tcctctggaa gtgatttatt ttattttttc cggagaagtg gaagtaacag gacctactta
    48661 tcctaaaggt cgctatgttg ttcgcgagtt acaaatttta ggtttgattg atatgtttaa
    48721 agaagaaacc cgcagcagtt cagcaaccgc attaacagaa gtgactgctt atgctgtttc
    48781 ccgttatgac ttgagtgatt tgttccggca gaatcctaat ttaggtgtta aaatgttaac
    48841 tgcattctgc caaacattga gcaattatat ttttaatatt tcatctccgg aaagcgcaaa
    48901 agaactatga actggacgaa gtggattttt tatagtttat tactggctct gattgtgctg
    48961 ggattgtttt tttaccatcc cataatttac tatttaattt atgcctttat tttttcctat
    49021 attcttgatc cttttgtcag ttggtttgag agaaaaaggt ttccccgctg gcttgcagtg
    49081 ctgacaattt atataataat tgcgggaatt attgctttgt ttacttctcg tttggttccg
    49141 gctttagttc aacaagggaa caaccttttt aatattttaa gtagtgggga acagctaaca
    49201 ggacaatata ttataacctt accttttatc agaaggatat acgaattttt acaggaattg
    49261 gatgctcaaa ttcccggttt gggaatggca cctaagctga tagaaatatt agaccaaacc
    49321 aatgtctttt tggctaaaat tcctaagtta ctgatagata actataaaaa gatattttcc
    49381 acaatctcta ttattttcac ggttcccctg atttctttct ttctcttgaa ggataaatac
    49441 aaattgagga agggaattgt aaaaatgttt agcaatcgtt attttgaact ctgtatcatt
    49501 atgatgcgga aaatagacag caccataggt aactttttgc gagctatctt gtttgaaata
    49561 atagcagttg gcattttagc ttctactgcc ttaacgattg tgggagtgcc atattccatt
    49621 ttaattggtg ctacggcagg agtggcaaat ataatacctt atttcggacc ttttttaggt
    49681 ggtgctttag ctgtggtaac agttctgttt aatggtggca ctattctttc tgcaatcacc
    49741 gtaattctgg cgatgtatat cgtgcagttg attgataata atattgttta ccctgttgtt
    49801 gtaggcggaa ctattaatat gcatcctttg ttagttcttc ttactttaat tgccgggggc
    49861 tggtatggag gcattctctg gatgcttatt tcggtgcccc ttgtgtattt aacatataat
    49921 ctgattaaag ttttgtatac taacctgaaa ggttttaaaa tcatataaaa gaggaaaatg
    49981 tgagtttact ggataaatgt tacaagttta cagacgcacg taaagtgatg gctatgggtt
    50041 attatcccta ttttcgggaa attacttccg agcaagatac ggaagttatg tgtaatggta
    50101 aaaaaatgtt gatgttgggt tctaatagtt atttgggctt aactaatcat cccaaagtga
    50161 aagaagcagc cataaaagct attcaaaaat atggtagtgg ctgtgcagga agtcgttttt
    50221 taaacggaac cttagatatt catctggaac tggaaaacga actggcaaaa ttggtcggta
    50281 aagaagaagc aatttgttat ccaacaggtt accaggcaaa tttagggtgt atttccacta
    50341 tggtaggtaa aaatgaatat attattactg ataaatacga tcatgcctca ataatagatg
    50401 gcgctattct tgccgaagga aaaatgctgc gttacaaaca taacgatatg gcttctttgg
    50461 aacgcattct taaggagatt aaaggcagga attctttaat tgttgtagat ggtatctttt
    50521 ctatggaagg ggatatagca aatcttccgg taataattga actggcggaa aaatataatg
    50581 ccgaaattat ggttgatgaa gcgcattctt taggtgtttt gggaaataag ggagcaggaa
    50641 ctactgctca ttttggttta attgataaaa cagcatttat tatgggcaca ttcagcaagt
    50701 ccctggcatc tgtaggtggc tttattgctg ccgatgaagc aattattcat tacttaaaac
    50761 ataaatcccg ggctcttatt ttttcggcat cacttcctcc tgcatcaact gccagcgttt
    50821 tagccgcgct taaaattatg caggaagagc ccgaaagaat tgccaaactt tgggaaaata
    50881 cgaaatatat gctgaaagaa tttcaagcaa tgggttataa taccggaacc agttgcactc
    50941 ctgttgttcc gttacatata gggaatatga tgactacttt taatatgtgg cgtcggcttg
    51001 gagaagaagg tgtttttata aatcctgtag ttcctcccgc tgttcctccc agttcttgtt
    51061 taattaggac atcttttatg gcaacacata ccaaagatca gcttgatatg gctctggaaa
    51121 aattcagaat gattggcaag gaactaaata tcatttaagt tataataggt atcatctcaa
    51181 attttttgaa catataaaat aagccatata tggaggaata aaatggcaag caagagcagt
    51241 gtggaatatt attccaacat taaagaaatg tctcctatca gagctattgc cgaaaccctg
    51301 atgaataacc atttagttcg gaaagtggat attcgcgagg catacgagct ggcaaaaaat
    51361 caacctggcg taagtgttac cgatttgcct atgtatcctg agtttgtaaa gctgcataat
    51421 ctgcctgcag atgcaaaagt tatcaacgat tgtcatggta acattgttgg cagaaccgct
    51481 aaagctcgca gattttacca taggttaagt ccttccctga aaaataagct ggaaagtgat
    51541 tttcgggaag cagtgtggca aatgcaacat tatccattaa ttaaagccga agcagttttg
    51601 gggatggata aagacctgat gctgaaagct acttttgtaa ctactgaaag tgatgctgcc
    51661 aatcttttct tttggctgtt gaattttgcc ccttatgaat cgttgaaaga ggaatatgag
    51721 gcaagtccca agcttcctat tcaggatatt attctggttg ctttcaatga atggacttgc
    51781 gatgacgatt tttacaacaa tgtaggggca ccacaactgg ctttggtgga tgaaaaacac
    51841 aatgttatgg ttaatcttgg aatgcgttat tttggtgaac ggaaaaaggg aactttaact
    51901 atggcttgga cttccggcat cagaatcggg atggctgctt gtcatggagg aattaaagaa
    51961 attgatttca gcacttgcgt aaatcctgaa tatcataaat tgggtaagag gtctatcgct
    52021 ttttatggac tttctggaac aggaaaatcc agccatacta ataatgcgga taatgcagga
    52081 accttgcctg agggcttttc caaggttgtt ttacacgatg atgctttcca gattgatttg
    52141 gaaaataagc tgtgtcgcgt ttgggaaccg actttatttg acaaaacgga tagccgaacc
    52201 gttgatcatc ccgactggaa atatactttg gctttgatga atcatacggt tttggatgtt
    52261 aatggcaaac ttattcctta cggacaggat attcgtaatt ccaatggcag agcccttttt
    52321 gaccgttctc ttttgggaaa atatgtgaac cgctgtgctt ttcctaaagc attagtttgg
    52381 ctgatgaagg attctatttt gccacctgcc ttaaaattgg aggataaata tttggcaata
    52441 gctatgggag ctgctttaat gactcagcgt aatcgtgcgg aaaatgttgc cgaagaagaa
    52501 cttaacaagc tcgtttttga accctttgct aatccatttc gggtgtatga actctatcgt
    52561 gatgtggaag catttttaaa tgtggctgat acaggagcgg atttttactg ctttaattca
    52621 cgtggctatt ggagggaatc agacgatatc ctagaaccga tacctctaac tacttcgtta
    52681 actctgcaaa ccgctatttt aatggaccag ctgcaatggg aaccctggga tttattacct
    52741 ggctctatga ttcctacaag agaatcagtt gaggcaattt tacccggcta ttacgacaga
    52801 tatcattcca acctgcgtat taactacgat aaatatattg ccgtaatgaa ggacagattc
    52861 cagcagagaa gggatttctt actaaatagc gatttgaagg aaagacccga aatacaggaa
    52921 aaactcattc aagcattgca aataaatgct taaacaaaat atccgggttg ccgatatttg
    52981 atttggaagg ttgatgtctt catcagccat ttaagtcagg agtcatttac gccttggtat
    53041 cttttagctg ggaaataact tctatggcgt atatgactcg tgaagacaca gattaaggta
    53101 tcttacttta atggtctttt gaactttgtg gcaaaacgga ttttacaacc tcatttctca
    53161 atgaatagca ggagtcatca cctgctattc tttttatatt cttgcatagt tatatatggt
    53221 atagatatgc agccctctct cggtattttg ccgagtatca ccacggtata cataccatag
    53281 ataactaaga aaggaataag gcgttctccg gctacacatt atttttacta cctacttctt
    53341 gatacctgaa atgctttttt tattcaataa agccaccccc caaaagcaaa tcctccgcgt
    53401 ataaaaccaa gccctgtccc ggggttatag aaagctgcgg ttcatcaaaa ataacatcta
    53461 ttttgcgatc attaatagga gttacaacac attctgcggg agtatgcaga aagcgtattt
    53521 tggcttttgc cctaaattta ttttcgggtg caggaagaga aacccagttc atatccgttg
    53581 ctgaacattt ctttt
//
LOCUS       DAOOZV010000004        52127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_750_length_52127_cov_63.626671, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000004 DAOOZV010000000
VERSION     DAOOZV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 52127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52127
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_52127_cov_63.626671"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            689..4624
                     /locus_tag="PLW08_00930"
     CDS             689..4624
                     /locus_tag="PLW08_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPL51203.1"
                     /translation="MPKQNTCNKLLLGIIALIFALNLKAAMVNNLPVTVFQPDGTKLE
                     LLASGDEYHNWLHDKNNYTIIRHPESGYLCYAEQDRENVKASNLIVGKDDPFRSALKP
                     GINISEEAYKELRSSKFWMPAERDAPTTGTINNIVIFIRFSGESEFGQGISVYDGWFN
                     SGTNSQKNYFLEASYNQLTVNTTFYPPAQNNLVVSWQDSHPRAYYEPYNATTNPTGYN
                     GDDERRNREFTLLQNAVAGVAAQVPSSLNIDSDGDGKVDNVVFLVSGAAGEWSSLLWP
                     HRWAIYDRNVYLNNKRVYDFNLQLKDFLATRGVGVICHEFFHTLGAPDLYHYTSNGID
                     PAGSWDLMCSDQNPPQHMTAFMKWKYGNWIATIPTIFIGQQYTLNPLTSSTGNCYRID
                     SNNPNQYYVVEFRKKTGTFESSIPGSGMLIYRIDTTAGDGNASGPPDELYIYRPNGTT
                     TVNGTINSANFSQETGRTSINNTTNPAPFLQDGSEGNLNIYAIGSSAGTTMTFSLGVP
                     TVDFSTNPYTESFDSSTFPPTGWYKQTISGSYGFERVTSGTHPTCSPQAGAGMICYKS
                     YNALSSNSAILATLRLNCSEVQFYSYDFSFYMYRDTGKSSNADRILIYLADTPDMSGT
                     NTLLGTINRYTGFTPTVPSNGWYQYSFNLPLTTSGYYYVVCQAISAKGNNMFLDSFSI
                     KKTALPPSPAISPSPADLAENVSVSPIFSWANGGGYPSHYLFYLGTNNPPTNLIDGLN
                     LGNVLNYAYTGTLNYSTQYYWKVIPVSEGGSAQNCSVWSFITYADPRITTLPYRENFD
                     IVTAPTLPFGWETYISSSNSSAFVKNFSSPTNYALSPPNCINFNNITDTSAQLILITP
                     EILTPINILRLKFYAKGSATGYSLQVGTMSSPNGTFTSLTTVNLTNTMQQYSVPFSNY
                     NGTDRFIAIRHNLGGINRNIYLDNFYLEEELTNDLAISALQMNGLGVIGEPLQFSVSV
                     LNNGIATQNSYSIQLLSNSAKTVLANLDVLEPLAPGQTAVHNLTWIPETEQTLSVYAQ
                     VTLISDGYTGNNTSIPSPISIGSYIPKIGTTDTGTKSNLLPLNFNQKNSISETIYLAS
                     ELQMTAGIITAIAYHTNFVQDLSHQAVKIWMKNTPESNLSSGWLSFDEYVPVFDGFVD
                     FPAGSNTIIIILQIPFPYNGNNLAIRVNRPFDNNNYNTSNHFYYTNSTSNPGRSRYLS
                     SSSVNYDPQNPSTLGTLTNFIPVTGFSVTSYIPAVLAIPQLLIQKNQQGVLLNWNAVQ
                     GANLYRIYASSDPVVWSEIPYAECSQTSFLIINPPDKMFFKIVAVYQAP"
     gene            4752..4827
                     /locus_tag="PLW08_00935"
     tRNA            4752..4827
                     /locus_tag="PLW08_00935"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4785..4787,aa:Phe,seq:gaa)
     gene            complement(4880..6049)
                     /locus_tag="PLW08_00940"
     CDS             complement(4880..6049)
                     /locus_tag="PLW08_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51204.1"
                     /translation="MKVLIIPSWYPYPQKPNTGRFFLDQAIALSRNSNYEYEILNWGQ
                     NEYQLQIRHPFAAVSKIVNYLTAKKETRNITPNLKEIKIPHLTWTSRLFYGNIKALVK
                     KLDSLSRPDIIHSYVTFPAGFIALRLVEKWKIPYIISEHSGPFPFPEYVIRNKVSKKI
                     TLPLHNASAVLAVSKFLQAQILSSCSVQSIVIPNLVDTEFYQPAKIKEERKRFSFFSL
                     SILTYAKGVMDLVDAFALAVQKGLDADLYIGGDGYLRSKIKQKITKYHLEHRLHLLGY
                     LNPQQALEQYQKCDCYILPSHIESFSLVLLEAMACGKPTLATDCGGPRDIVTPETGIL
                     IPPKKPFLMAEAMLKMKENINNYSPARIREICITNYSPPVICKKITAVYQNILQK"
     gene            complement(6046..7320)
                     /locus_tag="PLW08_00945"
     CDS             complement(6046..7320)
                     /locus_tag="PLW08_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51205.1"
                     /translation="MKNVLLIFYYFPPAGGSGVQRGLKFAKYLPEFGFRPVILCADYR
                     FLKQPRDYTLLRELPDKVKIYRSFTLDINWFYKLLWGLKLNKIVNFLRRNVFIPDGEI
                     LWLPFAIHKLKKIMATNNINQVLVSVPPYSLIFLAKYLKKHYQIKVCLDFRDPWSFGI
                     GRKYLKPPDWVTAIENRWEKEIVTRADQVICVSSVMIEEFRHLYPYLDKNKFVCITNG
                     YDEKDFPVSFPPIRNAKFTIIYTGSFYDELQPDILWQAILELIQEGCLNPQKIAVEIY
                     GRNFRNFVLGKYITDPILNQIVHFHGYINHRNSIQILRAADVLLLYLGSGEAQRAIVT
                     AKVFEYLRSGKPILAIIDSSGAAADILKPANTAFIADSSSIHSIKETLGNLYRLWEED
                     KLQTEPNWDYIQQFERKALTAKLAEVFSALEQ"
     gene            complement(7317..8069)
                     /locus_tag="PLW08_00950"
     CDS             complement(7317..8069)
                     /locus_tag="PLW08_00950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL51206.1"
                     /translation="MNYMAEATWENYDPEKQADLRIAKLLALLPSDVQTILDAGCGNG
                     IITNKLIAKYQVTGLDISPAALKYLQCPSVQASVTEIPFPDRSFDAIICNEVLEHLDN
                     ISLQKAFPELKRVTKKYIIISVPHKEQLEKLYYRCANCGFIEHPYGHLQSFSEETLST
                     YLAPEFYLKKREIFGPLNRDFIPLLLNIKQRWLKQWFNPASGLKCSRCQQEKFEINKS
                     LLTKGINLLNRLLVHPRPYWYMGLFIRKTQEL"
     gene            complement(8066..9529)
                     /locus_tag="PLW08_00955"
     CDS             complement(8066..9529)
                     /locus_tag="PLW08_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPL51207.1"
                     /translation="MSELPKQAGILSATEFFRLAIKAVIGIALAHLLTPSELGSYRQL
                     FLIYTTFSTLLLLGIPQSMLYFLPKAESPEKQNALITRCLNLISLLGLIFAFCLLIFR
                     KPIATLFNNPALEKLLILYALYPIFLFVTQFFSSVMLGLKQPFKAATFTIFAIIADFI
                     LIVGSAFFTRNLTIIVCGVISSAFLQWLYARISLAKFKAKGTRFDLSSFKTLMSYSLP
                     LGLSSIIGMMSIQLDKFMVSAFFSPEKFAVFSIGATEFPIVGILANSINSVLLPHLSS
                     SNPEKMGILYSGAVRKNTILVFPLMALCYIFAEPAITLIYGKLYTESAIYFRIYLFLL
                     PLRIATYGILFQAFGKTRFVMYNSLFLLISNAILNYLLIIKFGMKGAAFATVIVTWLS
                     VIIYLIQIAKVLKLNLMAFFPLEKIAKNAILTVICSIICVLIIKLGNSTIPFLLTGGV
                     VFTVAYLFLGRKIGVILDYDVQLVREMLTDFFSRFKK"
     gene            complement(9522..10970)
                     /locus_tag="PLW08_00960"
     CDS             complement(9522..10970)
                     /locus_tag="PLW08_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPL51208.1"
                     /translation="MKKGFFILFTILFVKINAEIRSVWVLPWDITTEKSIDEVIATAV
                     SCNQNELLVEVRYRADALFDTSKGAYLYPNPEPKSYILEDASFDPLAYILKKAHQKGL
                     AVQAWVVVFNATPREQSYIQQNYIYNNHKDWITYNFNGSQMNIDRQSGYFIDPGIPEV
                     QEYLLNILGNLAGGYPELDGIHLDYIRYPESDLGFHPVSLARYNEYCQNQEEITYNEW
                     RIMQVTNFVENAYFQLKEINPALQLTAAVFPDIAEANVDYAQDWQSWIKKGIIDRVYP
                     MAYDVQYAKFKKQLEQINLLQMKEKIVIGLRAWNGNGNSLAIGNGNSYNVKEIAKKIT
                     LTRDYGFAGVSLFSYSGLQKGNAFVQLARLSFPEKKEYPRGNISSKLNEITFSEPKYP
                     AEAKLTRLNSEIIMHLKIPKDGLWNWELYKDTLLVSNQRFYQKGENREILNIINPQIS
                     ANLEPGHYIVHLYQEKSSFQYLIPVELVTPNE"
     gene            complement(10967..12307)
                     /gene="xseA"
                     /locus_tag="PLW08_00965"
     CDS             complement(10967..12307)
                     /gene="xseA"
                     /locus_tag="PLW08_00965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPL51209.1"
                     /translation="MENVIIYSVYEITRQLRQVIETSLQPVFVMGEISGFIRHSSGHI
                     YFNLKDENATLRCTFFKGANASLTFQPADGMQVICFGKLTVYEKGGYYNLNVQNMQEA
                     GMGLLQQKFEQLKKKLEKEGLFAAEHKKPLPKYPDRIGIITSPTGAALQDIINIIKRR
                     YPVELIVYPALVQGSDAPPQIIQGIKYFNNTRDVDFIIITRGGGSQEDLFCFNDEALA
                     RAIYDSSLPVISAVGHEIDFTLADFVADLRAPTPSAAAELAVPDKKDLLNLLASLKKR
                     LDLSVQNNFTGKRTPLLNLQLELAKYHPERVWRGFQQRFDMAEMALHNALKQLPEKRL
                     QFEMKANNIMQQLNSRIELCLAKSKADCNLTWHQLNEKINNQLQFKTNRLNSLSEILE
                     QLSPRSIQSKGWIMAFKEGKLISSIKQVSEGENLSLDFADGIAETTVNKVEKRE"
     assembly_gap    12410..12509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12912..13484)
                     /locus_tag="PLW08_00970"
     CDS             complement(12912..13484)
                     /locus_tag="PLW08_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL51210.1"
                     /translation="MIFDLVYRNRSYRRFHEEKRLTRAQLIELIKVARYIPSAGNLQK
                     LRYFLIYEEDKCDLIFPHLKWASYLKNWNGPEKGERPSAYIIILAPVDSPKMVFIDTG
                     IAAQTILLSAVDNGFGGCMLASVDKQEVQNTFSLPEEMEIMLVIALGYPAESVVIEDV
                     TEQGKIEYWRDEKGIHYVPKRKIRDLIINY"
     gene            complement(14072..16069)
                     /locus_tag="PLW08_00975"
     CDS             complement(14072..16069)
                     /locus_tag="PLW08_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51211.1"
                     /translation="MLKYVLIVLFALLFSGFLNYRTNPEITKSRRWILFALRFITLFL
                     LLILLMSPILYYTQHKKQAPQILVLEDISQSMDLKHNSTTKSQHLKPLWQEMEKKFTA
                     SGYEVLHKTFANGLEGDKNNTLLSPALKELSRQKVFTNLEGVVLASDGWLRDESLSAV
                     QQLGCPFYVLADSSQNPAPDLAIVNVRCNRYAYRNEPNTIRAEISSQNYSGPAEVRLL
                     MANKIISRQNLKLEKGITANVDFTHKFNSIGFFPWKVEVAPLPNESQINNNIYPGAIE
                     VLAEKQRIILISDKPAWDNKFTLDAIATNSRWTAESYLNRNGRLYSGEVLVNKLEPDN
                     LAALIIINNGILNLDNATVSFIQNAYNRGVGILYQGLPVNELASLLPIQKSNITSTYQ
                     GFIVPTPISANYPMLNSLTLNAKDIPPLDYYYVTASPGSEILATINNPQNSPAIVVQT
                     TGTARSLAFATLNLWRWQMQSGDDGYTKLISNCLTWLSNKSTGTYNAIYNNSYFRGEE
                     IRIRLRSEDEIRQSRLDVNPRIRILDKNNKEVYTDFLKREGDEYAITLQLTEPGTYSF
                     EISDKETGKSTKGKFELSETSIETRDYGYNLSLLSWLAKETGGKLLYKSNLNTFNPVP
                     AKPVEQISRKEIPLYKKWYILSLFILTFCAELFLRRRWGLL"
     gene            complement(16069..17319)
                     /locus_tag="PLW08_00980"
     CDS             complement(16069..17319)
                     /locus_tag="PLW08_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51212.1"
                     /translation="MKKLALLLLFYLLFLTALNAFNTGKSVFFADSYMLRAKGVEAAY
                     WNPANLQQEKGIDFWIPIMNLGIGINNNSLDLDTYNYVVSRDTLNDIDKQKILNKIDG
                     SLRSALEGNISIVGFAFDNMSLSSSLHLTGKLALSERYLDLLLYGNTDSLYVFDKSTT
                     DAGGLGYVDITFAMGNFTLPYIPKTIPPIRAGFSVSALVGIGNANLDDFFGYFSSSME
                     GVDVKQDIVLHTGLGGFGQKAMLGLASNITPNLEVGLTLDNLFGKINWFLTTEETTIK
                     VVANDVYAANISEEIYDYANETVDIDSYSTKLPPELRLGTLYKIPWANFSLDYLQGFK
                     TSIATSGIGRFSLAAQFLPWEYLPLNIGVGFGNSHYPWRISYGIGYSSKAVEFGFGLQ
                     SVKNLIPGYKTKGITIGSYMKVHL"
     gene            complement(17321..18823)
                     /locus_tag="PLW08_00985"
     CDS             complement(17321..18823)
                     /locus_tag="PLW08_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51213.1"
                     /translation="MKKIYLMLIIALILLTACEIKKPTMPTWFMDLNIPIINERYYVS
                     DLVDSVNIVMGENEVLTVTTSGEATTNEFGTLPFTPDVNVEGLPIWSTGLDLTIPFED
                     QNGKVGVAYAEIAEGFFKSRFNNLQSGVEITLTFYDLYLPNNQNLVLTSNNPGNWVTT
                     NLEGLHLGVANSDQVINQIHLSFQITPSLPSGMQVATFDFQANETIQFGEFRGTLNNY
                     PIGLEDSGSLVNIDYPYGLDQAINLEDAYLQITLQNYLGFGSVFSGQLKATNSSGQSR
                     IIDILDEYGNNYQIEPATEEGPRIVEIQFGHNVAQLLQIMPTTIQVIDGQFLISSGTN
                     IGSLRPTDTMLLFYTVSAPLTFTLHAHEITIAEEQEFSIPEENRERIRKNLVNASLNL
                     ELKNKLPIGASAKLFFSTTPSIDTNNPSTYNFMKEAAIYSANLQPDFQNVNLTLNKDE
                     LNVFTSEQVFMRFAFSFEETGTPVTIHASTMDYIHIKGMMSARVLIEKED"
     gene            complement(19137..19904)
                     /gene="rnc"
                     /locus_tag="PLW08_00990"
     CDS             complement(19137..19904)
                     /gene="rnc"
                     /locus_tag="PLW08_00990"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPL51214.1"
                     /translation="MKSIISRILDYFNAKKITEQYPKWEKSLSQLQKKIDYTFHDPTL
                     LYAALTHKSYLHRHFEDHKTPSPFERMEFLGDSILGFIVSKELFTRHPDEQEGKLSKL
                     KSKIVSETYLTLKAKAIDLGKYLLLSPEEQQSGGASKPSILSDAMEALICAIYLDSGI
                     SAATRFIRNHILNNYQETVTREELVNYKSILQEYMQSRNQPPPTYVTVAEQGPEHHKT
                     FVVEVRWGNKLLGTGTGNTKKNAHQEAARMACQKLGI"
     gene            complement(19939..21180)
                     /gene="fabF"
                     /locus_tag="PLW08_00995"
     CDS             complement(19939..21180)
                     /gene="fabF"
                     /locus_tag="PLW08_00995"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPL51215.1"
                     /translation="MNKRRVVITGIGAITPVGHNVTQTWESLVNGVSGVDLISKFDYS
                     NLPVHIAAEVKNYDPEEHFDHKEVKKLDLYTQFAMIAAREAIADAGLIPGSFNPDRTG
                     VITGAGIGGILTFEEECIKNYTQGPRRISPFFIPKMIGNIAAAHISIENNFKGINFNV
                     MSACASANHALGTAFRSIQYGDADIILSGGTEAAVTPLSVGGFSAMRALSTRNNEPQK
                     ASRPFDAERDGFVMSEGAAILVLEELEHALARGAKIYAELVGYGASEDAFHITAPTED
                     GEGSSRAMLMAIQDAGLKPKDIDYINAHGTSTPLNDKGETISIKNVFGDYAYQVKINS
                     TKSMVGHMLGAAAGIEAIVCIKSIETGIIHPTINLTHPDPECDLDYTPNQAVKHIVNI
                     ALSNSLGFGGHNSAIIIKRYA"
     gene            complement(21348..21584)
                     /locus_tag="PLW08_01000"
     CDS             complement(21348..21584)
                     /locus_tag="PLW08_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPL51216.1"
                     /translation="MMDIEAKVKQIVVDKLGVEESQVVPSANFIEDLRADSLDTVELV
                     MAFEEEFGITIPDEDQDKLRTVGQAIDYLKEKLG"
     gene            complement(21627..22376)
                     /gene="fabG"
                     /locus_tag="PLW08_01005"
     CDS             complement(21627..22376)
                     /gene="fabG"
                     /locus_tag="PLW08_01005"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPL51217.1"
                     /translation="MNWDFSNKVIVITGAGRGIGFSLATAFGEAKAKVIIIDISAETV
                     EKAVAELKTKGFQVYGYVGNVTDSVNIENIFADIVDKYGTIDCLINNAGITRDNLLIR
                     MKEEDWQMVMDVNLKGSFICTQKAFKYMMRNRNGCIINIASVIGITGNSGQANYAASK
                     GGLIAFTKSCAKEFGSRNIRVNAVAPGFIETEMTASLSPEIRAEYAKAIPLQKMGTPK
                     DVANVCLFLASDAGSYITGQTIAVDGGLTMH"
     gene            complement(22413..23345)
                     /gene="fabD"
                     /locus_tag="PLW08_01010"
     CDS             complement(22413..23345)
                     /gene="fabD"
                     /locus_tag="PLW08_01010"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPL51218.1"
                     /translation="MKTAFVFPGQGSQYVGMANDFIQKDPALKEILDNFDKAHNTNLA
                     EVMASGPEEALKQTSYTQPAILFHSISALYGFQNKSKLQADFVAGHSLGEFSALVASE
                     MITFQDALHLVHKRGEFMIKANAGVPFAMSAIIGLEPNLVKEICSEAESVGLVRAVNF
                     NTPIQTVISGTAEGVQKAGEIAKAKGAKRVIPLVVGGPFHTPLIDKAKDWLMEEMQKV
                     DFKDGKIPVISNVTALPVMNGQEERELLARQIVSPVLWVQSVQYMIDQGVQRFIEFGP
                     QKVLSGMIRNINKDVEVFSIDKYEDIDGVVSATS"
     gene            complement(23455..24447)
                     /locus_tag="PLW08_01015"
     CDS             complement(23455..24447)
                     /locus_tag="PLW08_01015"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPL51219.1"
                     /translation="MLNYYAKLSSFGSCVPQKILNNYDLEKIVETSDEWIRTRTGMFE
                     RHIASENEAASDLAYEAAVRAIESSSVKYKDIDQIIVATISGDHPFPSTACILQKKLG
                     LKDIPCFDISAGCTGFIYALDVARQYVENGIVQNILVIGVEILTKITNWKDRNTCVLF
                     GDGAGAAIVSRAEPSEISRIIDSKIAADGSQGEYLIQYAGGSRMPASHETVDKNLHTV
                     YMEGNRIFKNAIRCMYASSDELLKRNNLTTADVDWIIPHQANLRIIEGLASKMKVPMS
                     KVIVNIEKYGNTSSATIPIAADEAIRNKKIRRGDIILLTSFGAGLTWGSILARY"
     gene            complement(24450..25445)
                     /gene="plsX"
                     /locus_tag="PLW08_01020"
     CDS             complement(24450..25445)
                     /gene="plsX"
                     /locus_tag="PLW08_01020"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPL51220.1"
                     /translation="MRIAVDAFGTDNAPFPEIEGAVLAIKEDLCEEVILVGDEEKIKH
                     ELEKFFYPQKRISVVNATQRIEMEDSGATAVRSKRDSSLVKTIELHNQGLADAAVSAG
                     NTGAVMSASLLIYGRIKNVLRPAIAVVFPTETGHQILLDMGANVDCTAENLVQFAKLG
                     SMYFKYFFQEEHPRISLLNIGEESAKGNATTKQAYQLLSQEKELNFIGNIEGKDIIKG
                     ITDVVVCDGFVGNIVLKTIEGVGFSIFEILKEQINKDWIAKLGALLSYPVYTHIKNKL
                     DHTEYGGALLVGLNGISVVAHGRSNAKAMKNAIRFAALIAKSGFVTNARNYFERS"
     gene            complement(25447..25626)
                     /gene="rpmF"
                     /locus_tag="PLW08_01025"
     CDS             complement(25447..25626)
                     /gene="rpmF"
                     /locus_tag="PLW08_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPL51221.1"
                     /translation="MAVPKRRTSKSRRDKRRTHKVLTPPTISTCKKCGEPKLPHHICE
                     KCGTYNEKQILTVKE"
     gene            26171..30997
                     /locus_tag="PLW08_01030"
     CDS             26171..30997
                     /locus_tag="PLW08_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPL51222.1"
                     /translation="MIIALFLSIMKRITVLLLGLFLACNFFAIARQDSSKQQPLYSAN
                     VVKIKLSQDAVNRSQLPINAYETREKTNFNELDQLFALNGIKSITRAHIAAKDQKWVQ
                     DTGFDRWFLVHLNGIKSVEEVIANLKNKRYIEEAIPEYYAYLHNVPNDPYFANNWGHN
                     NTAQLPVYIHGSHSGPGVGVIGFDSDAIAAWGETDFFGDPNIIIGILDTGVDINHPDL
                     RLVQGYDFGDNDNDPNDNSMNAGHGTCTSGIAAAIANNGIGVSGIAGGCSVMPIKVSN
                     SHGEISFTYAANALIYAADQNVDVVSMSFGAYGMEIGSDPAADSALSYAYNNGVVLFA
                     STGNNDEPAIAYPAKHPAVIAVGAASPSGERKNSNSSDGEYWWGSGYSFMEQDTAGAV
                     DILAPTILPTTDIQGSGGYTDANYYMWFSGTSCASPYAAGVAALVRSVNPGLSPAEVR
                     NIITSTATDMTINTFEGWDYWTGYGLVNAYQAVLAANPYLPQCKIVYPINNTGFVINS
                     LIPVRVEASDLDDRSITEVRFFLDNNPDYVFSDATMPYEWIWNTAEATLGMHKIKVVV
                     IDNDGNQRQDQIVISIVSPADEGFETGDFSSLFWNNNSPQPWYVQDDIYFTGAFSARS
                     GDVENGSLSEMKLKINVTQPGDISFYRKVSARKEFSYLRFYIDDNLAVQWTGKRDWEL
                     VSCPIEPGLHTFSWTYYSYEGSYEYDNCAWIDHLILPEYENYYWPPRNLTAAGGNGFI
                     LIYWQVPEAGNPIGYKIYRNDVLLTTITGTRYTDTDVTIGVPYQYKIVAVYADGESES
                     TSPQTATPIAEDVTEAIIGTGTTATGSAEGCPINNLRKSLHGQMIYKASELNLQGIFG
                     PIYISRLGFNIVSSPLYSLPAFQIRMAHTAAENVSQWRGEENLITYYFADNYRPVPGG
                     FEMLTLDTPFRWNGIDNILIDTAFSPANATSSYGTVCYTVTPNGYRYTRSDTNDQTDI
                     FYGGTLTVYRPNLKISTANMPNIQISANNLVFGNVLVGFETSKSFTITNTGTSLLTGE
                     ITVPNGFSIACRGKKQNTVFFSISADNSAVFTVFFTPESPGTYNGNLIITHNVDSENR
                     IIVLKAISLTAQNTPFIDGFETDSCQWTFANENQTNKWMRGSLVTQAGRGGLFISGDG
                     GISHTYITNSASVSYAYKDILIPEGTENCKLRFSWKAKGEGTGPYIDYLRVYCVEPDV
                     VPEAGIVLSTGQLSSPLNHTEIWKEADLNIPNSLSGGPKRIILMWTNDATGGTQPPAA
                     VDNIRVLLNNYTDYAFVYNGLALVNVPAVSDTLGNTYYPKLTIEGITNPENYLTVTSG
                     FASLDEPFADAGMDFTLTGANFSGAEIIFYHNLGFTPYYLGYKIEGRGIWMIQSAEIN
                     WTNEYAYFTVPDFYPNATKISFCFPRQNNVLPPYLNSFTATLNESHKVVLSWISNAET
                     GISAFQIWRGNSSNLSSAIAISDLIPFNPAQQNNYTYTDTTSAALTRYYYWLKVINND
                     AVENFWGPITILTSNDPPPLPPPETALLGVFPNPFNPGTKINYALKQEGTVEFKIYNA
                     KGQLVNSFSETHSSPGTYVCEFSGKNKEGKELASGLYICSMRFKGKTYVRKMVKTK"
     gene            complement(31174..32334)
                     /locus_tag="PLW08_01035"
     CDS             complement(31174..32334)
                     /locus_tag="PLW08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51223.1"
                     /translation="MVKREMQDHLLHLCKGFPIITITGPRQSGKTTLAKMTFPDYYYL
                     DLENLNTRRAAKEDPSSLFANKQADYIVDEFQYAPELLSTIKSISDEVQKETQFILTG
                     SNQFTLMKDISQSLAGRSAIMELLPFSLQEAYPKEKQSLNTCIFRGFYPRLLVHNIEP
                     TVFYDSYIRTYLQRDIRLLSNIQNLDDFTRFLSLCAGRTGSLLNKESLANEIGVDSKT
                     IGNWLSVLQTSYIIYLLKPWQKNISKRLIKSPKLYFYDTGLACNLLQIRNETDLANHP
                     LRGNLFETFILSEVLKFFHNRCIQPPLSFYRESNGTEIDLLIEKGNLIYPLEIKSASV
                     INNSFYRNILKLSKSNLPLGKARIVYGGNQTWENDICKHIGWQDLSSSLTLL"
     gene            32693..35341
                     /locus_tag="PLW08_01040"
     CDS             32693..35341
                     /locus_tag="PLW08_01040"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPL51224.1"
                     /translation="MEISKTFEPKDLEKKWYQFWEENGYFKPRENTAQKPFTVLMPPP
                     NVTGILHIGHVLNNTLQDVVVRYHRMKGEPTLWLPGVDHAGIATQNMVEKELAKQGIT
                     RQELGREKMVEKIWQWKNEKGNIIIDQLKLLGASCDWDRLRFTMDEMLSRAVKEVFVT
                     LYEEGLIYKGKYIINWCPRCVTALANDEVEHTDENGHLWYIHYPFAEGDGYVTVATTR
                     PETMLGDVAVAVNPKDERYSSLIGKELILPLTDRKIPLITDEYVDRDFGTGCVKVTPA
                     HDPNDFEIGIRHNLPQILVMDETGIMNANAGKEFQGLDRYACREKVLKMLTEQGLLEK
                     TEIHSYSVGRCYRCDTVIEPYLSDQWFVKMAPLAEKAIEVVEKGEVKFQPERWTKVYM
                     HWMNNIRDWCISRQIWWGHRIPVYYCDHCSYMIVAKEMPEKCPKCGGTKFTQDSDVLD
                     TWFSSWLWPFSTMGWPDETADLKRYLPTNVLITAPEIIYLWVARMIMSTLHFVDKIPF
                     DTVLLHGTVRDEIGRKMSKSLGNSPDPIDLINTVGADALRFSMIFGTPKGADVIFSES
                     ILETGRNFANKMWNAYRFIMMNAENIEGLPAENELQLELADKWIISRLQEVIIAVCAH
                     YENLRFNDAATEIFKFMWDEFCSWYIELSKDRLNPSSALSGRLTAKYILLYVMQTGMR
                     LLHPIMPFITEEIWQGIKKIFPMEEEALIIATFPETDETLIDNDIDNNMRFVQESITA
                     IRNLRKQINLAPNQEIDIVIRFAENSQQKLFENYMTYFNRLAKVKTLSGGVNIAKPKA
                     AIASVVRNIEIFLPLSGLVDIESEKNRLGKQIEKLTKEMSGIKAKLANPNFLNNAKEE
                     VVAKEKEKFIEVNTKLELLKNLLSEL"
     gene            complement(35642..38143)
                     /locus_tag="PLW08_01045"
     CDS             complement(35642..38143)
                     /locus_tag="PLW08_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPL51225.1"
                     /translation="MAGKNVNQKIRQNISKTSKTMPSAKTTAREKEPSAFAKHLPWVL
                     VIILFLLLSLIYFPVAYQGKAPQASDIIQWQGAAKAIIDYNATHKDNALWTPYMFSGM
                     PSYMIHFPNRYPFLENITKLTDKIINWRIFLLFIGGVGIFLLLRQLKMDPWIAFFSAI
                     AFTFSCHWVGLLEIGHNTKFRAIMYIPWVVWALFRLKEKPNILNLGLLATFLITQLRE
                     NHPQITYYLYLFIGMFWIYGLIEAIKTKQLKKFGNWTGLLIIAFAVTALAVMNPYLST
                     WEYSHYTMRGGSTGLEKSYAQQWSFPPVEIISFIIPDFFGGINQNYWGAMPFTQIYNY
                     FGIVVLALGVIALFGKHKKFSLFLWIASAIFLVLSFGSFTPVISGFFFKYLPMFNKFR
                     VPSMTLIMVQFIAVILAGLGLDTIASMENNSKWQKKLFTAFWISGVVFILFLILGQSI
                     FKGLPYTNAAEIEQYQRYNALAKLEELKSLRPGMLYKSGIMSLLLLTVSLGICYLASA
                     KKLKKVALVLLITLITFIDLYIYTGKHLKDLYPAEEREARFVAQDFDEFLLEDKDNYR
                     IYPYNMGQIRTAGEWAYYHQTIDGYSGAKLKRYDDVWKIIQGDDKNYSEFYRYLNGVY
                     EKGGVEIPTPLLDMLSTKYIIYPDSLPYAFLLNKIKPVFTSFSGANIYQNLTAYPRAW
                     FVDSLSVIPEIEARLQKMREPSFNPRSLAIVESKIDGVFKPDSCFAKETYFDLHNVKY
                     DVATDKNAFLVVSEVYYPAGWKAFIDGKKTEIYPVNHILRGVIIPPGKHTLEMKFISE
                     TYKLSLTLSLVGILATVLLLVAGIGWEEKKKKLKK"
     gene            38241..38642
                     /locus_tag="PLW08_01050"
     CDS             38241..38642
                     /locus_tag="PLW08_01050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPL51226.1"
                     /translation="MIFAFTKRIYGYECDIYGHLNNANYLQLLEGARSEALYEMNLAQ
                     SDLQKKGVQLFVHYVEMSFIKSIDHDELITIKSCFTEMNRIKGKWHQEIYNSKGEKCF
                     NADITIVFASEGKAKRLPVEVYELFMHYLEK"
     gene            39578..40048
                     /gene="rimP"
                     /locus_tag="PLW08_01055"
     CDS             39578..40048
                     /gene="rimP"
                     /locus_tag="PLW08_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPL51227.1"
                     /translation="MEFYRAQVEEIARKICQEMHLAVYDIEEKMSGKGRIITVYLTKI
                     GGVTLDECAAFSNALGKELEIFDLIPERYFLEVSSPGLERPLKLKSHYVSAINEKVAV
                     QFQAGEERKSIMGTLTEVNQDTIVIDDRGSVTEIPFKSIIRAKTVFLAIPKRRD"
     gene            40052..41287
                     /gene="nusA"
                     /locus_tag="PLW08_01060"
     CDS             40052..41287
                     /gene="nusA"
                     /locus_tag="PLW08_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPL51228.1"
                     /translation="MSANMLDALNKLAAIKQLNKDTIQTIILEAVTSTLQKRLEPEAG
                     LQVYIDELAGCVKVKFKSLVVEREEGLGQISLIDARNNYNHNAQLGEYIEKTMTLSEF
                     EPKIVKTVQKIIQDKIRQLEEEKIQNDFNKQKHTIVTGKIKAIDDYGGYIIDTGHVDA
                     LLPVDEQIENEFYRVGDNIKAYVVNIRTQKDRVVIILSRTNPEFVKKLFEAEIPAIFS
                     GEIKIRKIVREPGIRTKVELEAEDPKVDPIVACVGPKGTRIDSLRKELHGEQIDIVLH
                     SDDPEKMIENALGVEGIKRVIIERNHSASVILDEADKLMAIGKQGKNVKLAAKLVGMK
                     IDIYTMAEFEEKMAKERRTVSHITELDGVTPKIAETLKQAGYTSVQDIYMASLEELCN
                     LEGMGQKTAERLKEAAKYF"
     gene            41287..41517
                     /locus_tag="PLW08_01065"
     CDS             41287..41517
                     /locus_tag="PLW08_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HPL51229.1"
                     /translation="MPNKNSKAGHIPIRTCVVCKKKVDQKQLLNFFLTESGIVFDCNR
                     IIQVRKFYLCPVADCFKGLAKWRKSHQKRKIR"
     gene            41514..41843
                     /locus_tag="PLW08_01070"
     CDS             41514..41843
                     /locus_tag="PLW08_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPL51230.1"
                     /translation="MNPKILTLMQFARKAGKLVSGFNACERAIDKKDIFLLILALDSS
                     ERTREKMKKMAANSVISTSVIETGSQAEISSALGLPLTGVFGIKDKNFAGKIMEYWLS
                     DTERRRH"
     gene            41840..44380
                     /gene="infB"
                     /locus_tag="PLW08_01075"
     CDS             41840..44380
                     /gene="infB"
                     /locus_tag="PLW08_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPL51231.1"
                     /translation="MTIRVHELAKELKISTMALKKHLTDLGVNVKSHMSLVEDEVADK
                     IRLKYKEQIDAEKRAERDRKRLIEMRQAAKAQKSAQEAETIKAEEPVPPVVEPVKEVP
                     KKEEPKPVKEPEKETPIPVKEKPVEVKPEEKPHKPAPEKPKAVPKPKPEVIPPKTAAP
                     AKPEPWKKQEPSAPSIKPIPTPPSVEIKKEEKKFGKAKISHEELGEKSKHKKAIISST
                     KKAKQKLIETIEIDEAEISRNIKKTLQKSSKRKKYHREAQYVEDRSNEIVIREFTSVS
                     ELAKIMNVSPSDIISKFFMMGQLVTMNQRLDRDSLEMICDEFKVDYRFEDEYGMDIID
                     KEREQYNNVKEEPRPPVVTIMGHVDHGKTSILDYIRNTNVVAGESGNITQHIGAYQIE
                     INKHKITFLDTPGHEAFTAMRARGANVTDIAVIVISATEGVKAQTREAIDHAKAAGVS
                     IILAINKVDLPEANVDKVIAQLMEIGVYPEQYGGDIPWCRTSVVTGEGILNLIELILL
                     TAEILELKAKRDVPASGVVIEAGMDARMGPFATILMQEGTLHKGDIVVCGAVHGRIRK
                     MENERGAELKVLYPSDVARVYGLSDTPKAGDILNQVDSEKTARNISTERQQIRLEREK
                     YQNKSSLQNIFARIKEEQINTLNIILKTDTDGSAEALADSFQKLSNEEVSVNIIHKSV
                     GGISEADVSLAAASDAIIIGFHVRPSHQARKLAEEEGVQIKLYQVIYDVIDDLQNALE
                     GMLKPKYEEQVIGSAIVKQVFKIKRVGTIAGCQVDKGVIQANCKVRLYRNDVLVTEDT
                     ISSLKHYADDVKEVRAGSECGLTLANFSDIKEDDVLEAYIVNEVNKKL"
     gene            44380..44865
                     /gene="rbfA"
                     /locus_tag="PLW08_01080"
     CDS             44380..44865
                     /gene="rbfA"
                     /locus_tag="PLW08_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPL51232.1"
                     /translation="MKNYRIPRLQAELKKLFNIALSQKLNDPKLAWVQITDVVISKDL
                     HYAKLYFSHYNNPASHDQIRELLIKSSGFLKKQIAGAQIMRTIPELSFYYDDTEDRAA
                     KVDTLLASLRDDFDDEDEEDQDIDIDDILDDDDYFDFDEEDDEEDYDDYEDEEEDEEE
                     E"
     gene            44945..45946
                     /locus_tag="PLW08_01085"
     CDS             44945..45946
                     /locus_tag="PLW08_01085"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPL51233.1"
                     /translation="MQNIREELVELISKAENILLTTHISPDGDGYCSALALQRIISFL
                     GKSSLLVTDDDDLSRYNYLQDGKSQEKRFSELKEEEQNFSLAIVLDCNSYDRLGERCV
                     LVEKAEKIIVLDHHEAENGLIKADLSYIEPSFACVGEIIFSLLETAINKMPVSDRLFV
                     CNCLYTTILNDTNNFVNTNTDTDVFIFASKLVNLGVKPNEQYRYFFLQHSAEELRYIG
                     ETLSTIELHYQRKILFMYSTLAMSKKNNIDPESIMNATRWVQGIKGIEVITYLREEKE
                     RVYKISLRSETIDVNRIAVRYGGGGHKQAAGCYFYGTLAEAKDKLLKDVINALECHN"
     gene            45924..46805
                     /gene="truB"
                     /locus_tag="PLW08_01090"
     CDS             45924..46805
                     /gene="truB"
                     /locus_tag="PLW08_01090"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPL51234.1"
                     /translation="MPLNAITDKAFLAIDKPSGITSFAVIRRLRKITGSKKIGHTGTL
                     DPFATGLLICCLGSYTRLAGLLEEKDKTYLARMKLGIKTATGDPEGDIIQTAPIPEFV
                     SKLDALAKKARQLTELPVPAFSAVKVNGKRAYELARKGEKFNMPKRPVQIKEFEFLPW
                     EPNKSDELSYRCKVSKGTYIRALSEWLAEQLNTIACTTFLRRESIGNITLQDAVSLDD
                     LTKDNWFRYQLSYKKVFPDYTCLKLNADDFAKVMNGMDIAHSNENNEKYILLKEENIC
                     AIGQRMNNYIHPLRVLK"
     gene            46802..46930
                     /locus_tag="PLW08_01095"
     CDS             46802..46930
                     /locus_tag="PLW08_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51235.1"
                     /translation="MNKRKNEVLVVYCFPNYKVLDNNYNSEIMNFVWWTRTRYENE"
     gene            46917..47828
                     /locus_tag="PLW08_01100"
     CDS             46917..47828
                     /locus_tag="PLW08_01100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPL51236.1"
                     /translation="MKMSKKSVLTIGTFDGLHLGHQKVLKKVVEIAMAEGLQSVVISY
                     KDHPAFILKTHPMPKMLCPAEIKLQEMKKMGIERIELLDFTLELAKTPPLKFLKDYII
                     PQFQPEVIVMGYDSHFGHLRQGNYDFLCRHSEELGYRVEYVEPLLFEGKPISSSSIRN
                     LLLNGKIEAANYLLGRPYRLIGKVGHSNSKGRKLGFPTANLNLLCPHQLIPKSGIYLS
                     KVIWQENVFFGLTNIGCSPTVKNTGVIEIETHILDFQDNLYGQIMELDLLRYLREEKM
                     FKNVDELKTAIQNDIALARVMLKEFSG"
     gene            47877..49808
                     /locus_tag="PLW08_01105"
     CDS             47877..49808
                     /locus_tag="PLW08_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51237.1"
                     /translation="MRKVNKPTVYLAKRNLLFPLAVLFFLLLPVFFSAVSPLQVVPGS
                     GIEDIEDSKLYHFRYHNLVDDYHLSGPKIWAVRFNFKDAYPLADSASFTLNSFKIYSP
                     YPNLEMRVSLWNEQYGGNNELNYFPGVIIPGGNWQNITLSTSPTTITFPEIQNPLRII
                     WLVMDFTTPLADKYMSASEGSGKNSFYYNNTVQGNEFWQSLFTAGFKCELRVSAIGDF
                     NLLHTDLELLNFSLPENILPGSTVHPSVTVYNHSQYPVSDTLEVNFSDPLLELNQTLQ
                     IPLLNINPRDSLFTVSSEGISFSREPTQIKVTLAFKNHPNIIFSARYYNVFGETGSSH
                     LVEYFRRYTFGFSDIPQDASEVHHLLYFPNLADNLSNLFAVQRFNYYQFNSLPKTVVD
                     GYKKFHLQIDANSEELVSAISAAQVKRGFISRSSSNISIPDPETPENLLLNIKLYNDR
                     TTLYEWTASSALNPKFFAGIFEENEFAGGELYSLKRWLVFEGSFSGTLNKRDSVEIEL
                     ILNTTDLNPSKTYRVYYWLQNPFNSGGQIYYAQYVSLGQSWIVSNSDEYISQPVISVY
                     PNPLVKGNALKISASVFPAVFTVYNIKGQKVFSTGKVFSEISIPAGIFPASGIYFLRC
                     EMQDRDKTHKQIQKISIIK"
     gene            49818..50771
                     /locus_tag="PLW08_01110"
     CDS             49818..50771
                     /locus_tag="PLW08_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL51238.1"
                     /translation="MAEHISIAEMQQKVNQEISGLYLVAEKELREGKNDFYLRLKLQD
                     KTGTISANVWKDAVKHSDQFDTGDIISIQGTVVNYKGQIQLSLSQLRFADKSEYNIED
                     YLTRSKIEPEVLCDRFFEFVDKVHQPYLNKLLHLIFDDKEFFTQFLKAPAAKNWHHNY
                     INGLIEHTVSVAALCEFASTLYPVNYDLLITGALLHDVAKVQEYDHRRNIDFTDEGRL
                     IGHLALSDQMVSENAAKILGFPEELLLHLRHLILSHHGEYEKGSVRLPQTLEAIVLHH
                     CDNLDAQATGVAQLIAAIPQNAVWSEYDKLNNRYYKIYRPV"
     gene            50876..51661
                     /locus_tag="PLW08_01115"
     CDS             50876..51661
                     /locus_tag="PLW08_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL51239.1"
                     /translation="MFQTVIIASGSKGNCVLIQTENTALLLDAGISMHRIMECLESYH
                     IESAKINGILVSHEHSDHIKSVGAISRKLKIPVYINRPTLSSCAERLGDVQDRIVLFN
                     TGNSFTIGDIIIEPFVSTHDAAESCNFVFYPVSEPKRKLGVATDLGYPTQLCLLKLSG
                     VSTLILESNHDEKMLMEGPYDWKLKQRIRSLQGHLSNVQAVGLISALIHPGLKNLILA
                     HLSEINNLPSLAEQTMRDYLNSIRSDIKLFVAEQNRPTPPIEI"
BASE COUNT        16376 a         9618 c        10101 g        15932 t
ORIGIN      
        1 tgacgcagat taaaaatggc agagggctta ggggttaggg cagagagaaa ataggtatgg
       61 gatgaacagg attatatgga ttataaggat aaaaaaatag ttccgtagga acgacagata
      121 ctaacaacag gtttcaaccc ggtgataagg aatcaggata gtcattatct cttttcccga
      181 gaagaagttc cgtaggaacg acagatacta acaacgggtt tttaacccgg tgaaagaaag
      241 atggtaggta acatatatat ttgtaggaaa aagagttccg taggaacgac agatattaac
      301 aacgggtttt taacccggtg ataaggaatc agtaaagtca ttatctcttt tcccgagaaa
      361 gagttccgta ggaacgacag atattagaaa agggtttcaa ccctgaataa aaagtcaaaa
      421 ttggtatttg gttggcagca ttcttattta ccataaacca aacaatttat ttttcagagt
      481 gttgcaaact cttgggcaaa tcagaatttg cctacccatt cttcatctac tgcattattg
      541 gggataaaac tgctattctt aacgggtttt ttaggactta ccaaattgaa aattacatta
      601 tttgtgttta caaggttctg attgacaaat ttatggttga atgtaattaa gttttttcta
      661 aaagcaagca tacccaaaag gagacgaaat gccgaagcaa aatacttgta acaagctgtt
      721 acttgggata atagctctta tatttgcctt aaatttaaaa gccgctatgg ttaataacct
      781 gccggttacc gtttttcaac cagatggaac aaaactggaa ttattggcaa gtggcgatga
      841 atatcataac tggctgcacg ataaaaacaa ttacactatt atcaggcatc ccgaaagtgg
      901 ctacttatgt tatgccgaac aagatagaga gaatgttaaa gcaagcaatt taatcgtggg
      961 taaagatgat cctttccgtt ctgctcttaa gccaggaatt aacatctctg aagaagcata
     1021 taaggaatta cgcagtagca agttttggat gccggcagaa agagatgcac caaccaccgg
     1081 tacaattaat aatattgtga tctttatccg tttttctgga gaaagtgaat ttggccaggg
     1141 tattagcgtt tatgacggtt ggttcaatag cggaaccaat tctcaaaaga attattttct
     1201 ggaagcaagc tataatcagc ttacggtaaa tactactttt tatccgccgg cacaaaataa
     1261 tctggttgtt tcctggcagg atagccatcc gagagcttat tatgaaccct ataatgccac
     1321 tactaatccg acaggttata atggagatga cgagaggaga aaccgggaat ttaccttact
     1381 tcaaaatgct gttgccggag ttgctgctca ggttccttct tctttaaata ttgattccga
     1441 tggagatggc aaagtggata atgttgtttt tctcgttagt ggcgcggctg gtgaatggag
     1501 tagtttgctt tggccccacc gatgggcaat ttatgatcgc aatgtatatt taaacaataa
     1561 acgcgtttac gattttaacc ttcagctgaa ggattttctt gccacacgcg gagtaggggt
     1621 tatttgtcat gaattctttc atactcttgg tgctcccgac ctttatcatt ataccagtaa
     1681 tggaattgat cctgccggta gctgggattt aatgtgttcc gaccaaaatc ctcctcaaca
     1741 tatgaccgct tttatgaaat ggaaatatgg aaattggatt gctactattc caactatctt
     1801 cattggacaa caatataccc taaatccttt aacttcttcc accggtaact gttatagaat
     1861 agattccaat aaccccaatc aatattatgt agtagaattc cgcaagaaaa caggtacctt
     1921 tgaaagcagt attcccggtt ccggaatgtt gatttataga attgatacta ccgctggaga
     1981 cggaaatgca tcaggtccgc ctgatgaatt atatatttac cgtcccaatg gaacaactac
     2041 agtaaacgga accataaatt ccgcaaattt tagtcaggaa accggtagaa ccagtattaa
     2101 taatacaact aatcctgctc ccttcttaca ggatgggagt gaagggaatt taaatatcta
     2161 tgccatcggt tcttcagcag gaactacgat gactttttct ttaggtgttc ctacggttga
     2221 cttttcaacc aatccttaca cggagagctt tgacagttct acttttcctc caacgggctg
     2281 gtataaacag acaatttcag gcagctatgg ttttgaaaga gtaacttccg gaacacatcc
     2341 aacctgctca cctcaagcag gagcaggaat gatttgctat aaaagttata atgctctttc
     2401 gtccaattcc gcaattttag ctactttgcg gcttaattgt tcagaagttc aattttacag
     2461 ctacgatttt tccttttata tgtataggga taccggaaaa agttcaaatg ctgaccgtat
     2521 tttaatttat ttagccgata cgcctgatat gagcggaacc aatactcttt taggaacgat
     2581 aaatcgttat accggtttta ccccaaccgt tccttcaaac ggttggtatc agtattcttt
     2641 caatttgcct cttacaactt ccggttatta ttatgttgtt tgtcaagcta tcagtgccaa
     2701 aggcaataat atgtttttgg atagtttcag tattaaaaaa acggctttac ctccttctcc
     2761 cgcaatttct ccttcaccag ctgatttagc tgaaaatgta agtgtttcac ccattttttc
     2821 ctgggctaat ggaggcggtt atcccagcca ttatttattt tatttgggca cgaataatcc
     2881 accgactaat ttgattgatg gtttgaattt gggcaatgtt ctcaattacg catataccgg
     2941 aactttgaat tacagcacac aatattattg gaaagttatt cccgtaagtg aagggggctc
     3001 tgcacaaaac tgttctgtat ggagttttat tacttatgct gatccccgca tcacaactct
     3061 tccctataga gagaattttg atatagtaac tgctccaact ttgccttttg gttgggagac
     3121 ctatatttct tcaagcaata gttctgcttt tgttaaaaac ttttcttcgc caacaaatta
     3181 tgctcttagc cctcctaatt gcatcaattt taataatatc acagatacaa gtgcacagct
     3241 gattctgatt acccccgaga ttttaacgcc gataaatatt ttgcgtttga agttttatgc
     3301 caagggtagc gcaaccggat attcattaca agttggaact atgagcagtc ccaacggcac
     3361 ttttacttca ttaactacgg ttaatttaac caatacgatg caacaatatt ctgtgccttt
     3421 ttccaattac aacggaacag acagatttat agccatcaga cataatttag ggggtatcaa
     3481 tcgtaatatt tatctggata atttttatct ggaagaagag ctgactaatg acctggctat
     3541 aagtgctttg cagatgaacg gattaggagt tattggcgaa cccttgcagt ttagcgttag
     3601 tgttcttaat aatggaattg caactcaaaa cagctatagt attcagttac tttccaactc
     3661 cgctaaaaca gtgctggcaa atttggatgt attggaaccg cttgctccag ggcaaactgc
     3721 agttcataat ttaacttgga tacctgaaac cgaacaaacg ctttctgtat atgctcaagt
     3781 aactttaatt tcagatggtt ataccggtaa taatacttcc ataccttctc ctatatccat
     3841 tggaagttac atccctaaaa tagggacaac cgataccggc actaaatcta atcttctacc
     3901 tttaaatttc aatcagaaga atagtatcag tgagacaatt tatcttgctt ccgaactgca
     3961 aatgactgcc ggaataatta ccgccattgc ttaccacaca aattttgtac aggatttatc
     4021 tcatcaggca gtaaaaatct ggatgaaaaa tacccctgag agtaatctca gttccggctg
     4081 gctttccttt gatgagtatg tgccggtatt tgatggtttt gtagattttc ctgctggaag
     4141 caataccatt attataatct tacaaatacc ttttccctac aatggaaata atctggctat
     4201 cagggtaaac agaccctttg ataacaataa ttacaatacc agtaatcatt tttattacac
     4261 aaattctacc tcaaatcctg gtcgcagccg ctatctaagt agcagttctg taaattatga
     4321 tccccaaaat ccatcaactt tgggcacttt aaccaatttt attcctgtta ccggcttttc
     4381 tgtaacttcc tatattccag ccgtattagc tattcctcag ctgctaattc agaaaaacca
     4441 acagggtgta ttacttaact ggaatgctgt tcagggagca aatttatacc gtatttacgc
     4501 cagttccgat cctgtggtct ggtctgaaat tccttatgct gaatgcagtc aaaccagttt
     4561 tctgattatt aatccaccgg acaaaatgtt tttcaaaatt gtagctgttt accaagcacc
     4621 ctgagttgtt aaatccttat gagcattgtt gggaataaaa ttatcattcc ccttgctaaa
     4681 tatgtctatt aattacatac tatttttggc ttgacagaaa aagggtttat tttaggaaag
     4741 ttcaaaagtg tggcgagata gctcagtcgg tagagcagag gcctgaaaag ccttgtgtcc
     4801 ccagttcgat tctgggtttc gccaccattt ttttgtatag ttcaattttt ctgaattata
     4861 gggttgaaac aaatgagtgc tatttttgca gaatgttttg gtaaacagcg gttatttttt
     4921 tacatattac agggggggaa tagtttgtga tacatatttc tctgatgcgg gcaggggaat
     4981 agttattaat gttttctttc attttcagca ttgcttccgc catcagaaag ggcttttttg
     5041 gaggaataag aattcctgtt tccggggtaa caatatctct tggaccacca caatcagttg
     5101 ccaaagttgg ttttccacaa gccattgctt ccagaagcac caaactgaag gattcaatat
     5161 ggctgggaag aatgtagcaa tcgcattttt gatattgttc caatgcttgc tgagggttaa
     5221 gatagcccaa aagatgtaag cgatgttcta aatggtattt agttattttt tgtttaattt
     5281 tagaccttaa atagccatct cctccgatgt ataaatctgc atctaatcct ttttgaaccg
     5341 ctaaagcaaa ggcatctact aaatccataa ctcccttagc ataagttaaa atgcttaacg
     5401 aaaagaaaga aaagcgtttt ctttcttctt ttatctttgc cggttggtaa aattccgtat
     5461 ccactaaatt ggggattact atactttgaa cggagcagga agaaagaatt tgtgcttgga
     5521 gaaatttgct tacagctaaa acagcggatg cattgtgtaa cggcaaagtg atttttttag
     5581 agaccttatt cctgataaca tattccggaa aaggaaatgg accgctatgt tcactaatga
     5641 tatagggaat tttccatttt tccactaagc gtaaagcaat aaatcctgcc ggaaaagtta
     5701 cataggaatg aataatatca ggtcgggaaa gagaatccaa ttttttcact aatgccttta
     5761 tattgccata aaataacctg ctagtccagg taagatgagg gattttaatt tctttcaggt
     5821 tgggggttat attacgggtt tcttttttgg cggttaggta attaacaatc ttggagacag
     5881 cagcaaaggg atgtcggatt tgcaattgat attcattctg tccccaattc aggatttcat
     5941 attcatagtt gctgtttcgg gaaagagcaa ttgcctggtc aagaaagaat cttccggtat
     6001 tgggtttttg cggataggga taccaggaag ggataattaa gaccttcatt gttccaatgc
     6061 agaaaagacc tcagccagtt ttgccgtaag tgctttgcgt tcaaattgct gaatgtaatc
     6121 ccagttgggt tcagtttgca gcttgtcttc ctcccaaagc cgataaaggt ttcctaaggt
     6181 ttcctttata gaatggatgg aagaggaatc ggcaataaaa gctgtatttg cgggcttaag
     6241 gatatctgct gctgcccctg aggaatctat tatagccaga atgggttttc cgctgcgcag
     6301 atattcaaaa accttagctg taacgattgc tctttgagct tctccggaac ccaaatacaa
     6361 taaaagaaca tctgcagcac gaagaatttg aatgctattc cgatgattga tataaccgtg
     6421 gaaatggaca atttgattta gtattggatc ggttatatat tttcccagca cgaagttacg
     6481 aaagtttctg ccataaattt ccacggcaat tttttggggg ttaagacaac cttcctgaat
     6541 taactctaaa attgcctgcc agagaatatc aggttggagt tcatcgtaaa aactaccggt
     6601 gtaaataatg gtaaatttgg cattccttat tggaggaaat gaaacgggga aatctttctc
     6661 atcatagcca ttggtgatac atacgaattt gtttttatct aaatagggat aaagatgtct
     6721 gaattcctct atcataacgg aacttacaca gataacttga tcagcacggg ttactatttc
     6781 cttttcccac ctgttctcta ttgcggtaac ccaatcagga ggttttagat attttctgcc
     6841 aatcccaaaa ctccaggggt ctctaaaatc caggcagacc tttatctgat aatgcttttt
     6901 caggtatttg gctaaaaaaa taagagaata gggcggaact gaaactaaaa cctgattgat
     6961 attgttggta gccataattt tctttagctt atgaatcgca aacggtaacc acaggatttc
     7021 tccgtccgga ataaaaacat ttctgcgcag aaaattgact attttgttta gctttaagcc
     7081 ccaaagcagt ttataaaacc agttaatatc caaagtgaat gagcggtaaa tttttacttt
     7141 atctggaagt tcacgcagaa gagtataatc ccttggctgc tttaaaaagc gataatcagc
     7201 acaaagaata acaggtctaa aaccaaattc cggtaaatat ttagcaaact tcagtcctct
     7261 ttgcactccg gaaccgcctg caggagggaa ataatagaaa attaagagca catttttcat
     7321 agttcttgtg ttttcctgat aaataaaccc atataccaat aaggtcgggg atgcactaat
     7381 aacctgttaa gcaagttaat tcctttggtg agaagggatt tgtttatttc aaatttttct
     7441 tgctggcaac gggaacattt caaaccgctg gcaggattaa accattgttt taaccagcgt
     7501 tgtttgatat taagcaaaag gggtataaaa tcacgattca gaggaccaaa tatctccctt
     7561 tttttcagat aaaattccgg agcaagatat gtagataaag tttcttctga aaacgactgt
     7621 aaatgtccat aaggatgttc tataaaaccg caattagcac agcgataata tagtttttcc
     7681 agctgttcct tatggggaac actgataatt atatattttt tggttacccg tttaagttcc
     7741 ggaaaagctt tctgcaagga gatattatcc aagtgttcca agacctcgtt acagattata
     7801 gcatcaaaac ttctatcggg aaaaggaatt tccgtaacag aagcttgaac tgaaggacac
     7861 tgcaaatact ttagagctgc aggtgaaata tccaaacctg taacttgata tttagcaata
     7921 agtttgtttg taataatgcc atttccacaa cctgcgtcca aaatggtttg cacatctgaa
     7981 ggaagaagag caagaagttt tgcaattctt aaatccgctt gtttttcagg atcgtaattt
     8041 tcccaggtgg cttctgccat atagttcatt ttttaaacct actaaagaaa tcggttaaca
     8101 tttctcttac cagttgcaca tcataatcca aaattacccc gatttttcta cctaaaaaga
     8161 gataggcaac cgtaaaaaca acacctcctg taagtaaaaa gggaattgtg ctatttccca
     8221 gctttattat caacacgcag attatagagc atataactgt taaaatggca tttttagcta
     8281 ttttttccag cgggaaaaaa gccattaaat tcaattttaa gaccttggca atctgaataa
     8341 gataaataat taccgagagc caggttacaa ttaccgtagc aaaagcggca cctttcatcc
     8401 cgaatttaat tatcagaaga tagtttaaga tagcattgga aataagcaga aacaaagaat
     8461 tatacataac aaagcgggtt tttccgaatg cctgaaatag gattccgtaa gtggcaatgc
     8521 gcaaaggcaa caaaaagagg taaattctaa aataaatagc ggactcggta tagagctttc
     8581 cataaataag agtaattgca ggttctgcaa aaatataaca caaagccatt aacggaaata
     8641 ctaaaatagt atttttacga accgctccac tatataaaat acccatcttt tcaggattac
     8701 tggaactcaa atgaggtaac aaaacagaat ttatagaatt agctaaaatg cctacaatcg
     8761 ggaattcagt agctcctata gaaaacacgg caaacttttc cggcgagaaa aatgccgaaa
     8821 ccataaattt atccagttga atactcatca taccaattat agaagataaa cccaaaggta
     8881 aagaataact cataagtgtt ttgaaagaac tgagatcaaa acgagttcct ttggctttaa
     8941 atttggcaag agaaatcctg gcataaagcc attgcaaaaa agcagaacta attacaccgc
     9001 aaacaataat agttagatta cgcgtaaaaa aagcggaacc aacaatcaga ataaaatccg
     9061 caataatggc aaaaatggta aaggtagccg ctttaaacgg ttgttttaag cccagcatta
     9121 cggaactgaa aaattgggtt acaaaaagaa aaatgggata aagagcataa agaataagca
     9181 gtttttccag agcaggatta ttgaataaag tagcaattgg tttacggaaa ataagcaagc
     9241 aaaacgcaaa aattaaaccc aacagcgaaa ttaaattcag gcaacgggta attaaggcat
     9301 tttgtttttc cgggctttcc gctttaggaa gaaaatagag catactttga ggaatcccta
     9361 aaagcagaag agtggaaaag gtagtataaa ttaagaaaag ctgacgataa ctgccaagtt
     9421 cggaaggagt taataaatga gctaaggcaa ttccgattac agctttaatt gccaaacgga
     9481 aaaattccgt tgccgaaagg atacctgcct gtttaggaag ttcactcatt gggcgtaact
     9541 aattctaccg gaataagata ttgaaaagag gatttttcct gatagagatg gactatatag
     9601 tgtcccggtt caaggttagc gcttatttgg ggattgatga tattcaatat ctctctattt
     9661 tctcctttct gataaaagcg ttggttgcta acaagcagtg tatctttata tagttcccag
     9721 ttccacaatc catctttcgg gatttttagg tgcataataa tttcactgtt caaacgggtc
     9781 agtttggctt cagcaggata ttttggttca gagaaagtta tttcgtttag tttagaggag
     9841 atatttcccc gaggatattc ctttttttcc ggaaaactta agcgagcaag ctgaacaaaa
     9901 gcatttcctt tttgcaatcc tgaatagctg aataaagaaa caccggcaaa accataatca
     9961 cgcgttaaag tgattttctt tgctatttct tttacattgt atgaattacc atttccgata
    10021 gcaagggaat ttccatttcc attccaagca cgcaaaccga ttactatttt ttccttcatt
    10081 tgcagaaggt taatttgttc cagttgtttc ttgaattttg catattggac atcataagcc
    10141 ataggataaa cacgatcaat aatacctttt ttaatccagg attgccagtc ctgagcataa
    10201 tcaacatttg cctcggcaat atcgggaaaa acagcagcag ttaattgcaa agcaggattt
    10261 atttctttca gttgaaagta agcattttcc acaaaattgg ttacttgcat tatgcgccat
    10321 tcgttatagg ttatttcttc ctgattttgg caatattcat tatagcgggc aagagagaca
    10381 ggatgaaaac ctaaatcgct ttcaggatag cgaatataat caagatgaat tccatccaat
    10441 tccggataac cccctgccag atttcccaaa atattaagca gatattcctg cacttcagga
    10501 attcccggat caatgaaata accggattgg cggtcaatat tcatctgaga accgttgaaa
    10561 ttgtaagtaa tccaatcctt gtgattattg tagatatagt tttgctgaat ataactttgt
    10621 tcccttggag tagcattaaa aaccactacc caggcctgca ctgccagtcc tttttgatga
    10681 gcttttttca gaatataagc taaaggatca aaagaagcat cttccaggat ataactcttc
    10741 ggttcgggat tgggataaag ataggcacct ttggatgtat caaaaagagc atcagcgcga
    10801 taacgaactt ccactaaaag ttcattttgg ttgcaagaaa ccgctgttgc aattacttca
    10861 tcaatagatt tttcggtagt aatatcccaa ggtagaaccc aaacggaacg gatttcggca
    10921 tttattttca caaacaaaat ggtaaacaga ataaagaaac cctttttcac tctctttttt
    10981 ctaccttatt tacagtcgtt tccgcaatgc catcggcaaa atccagggag agattttccc
    11041 cttcagaaac ctgttttatg gagctaatta atttaccttc tttgaaagcc ataatccaac
    11101 ctttactttg aatactgcga ggagagagtt gttctaaaat ttcgcttagg gaattcaggc
    11161 gattggtttt gaattggagt tggttattaa ttttttcatt cagctgatgc catgttaaat
    11221 tacaatcggc tttggattta gccaaacaga gttcaattct gctattcagt tgttgcataa
    11281 tgttatttgc tttcatttca aattgtaaac gcttttccgg aagttgttta agtgcattgt
    11341 gtaaagccat ttctgccata tcaaagcgct gttgaaaacc tctccaaacg cgttccggat
    11401 gatattttgc cagctccagt tgcagattta agaggggagt gcgttttccg gtaaaattat
    11461 tttggacaga taaatccagc cgttttttca gcgaagcaag caagtttaac aggtcttttt
    11521 tatcgggaac tgcaagttca gccgcagctg aaggagtggg agctctcaaa tcggctacaa
    11581 aatcagccag cgtaaaatca atttcatgac ctactgccga aattacagga agggaagaat
    11641 cgtaaatggc tctggctaat gcttcatcat taaaacagaa aagatcttcc tgagaaccgc
    11701 caccacgagt gataattata aaatcaacat ccctggtatt attgaaatac tttattccct
    11761 gaataatttg aggaggagca tcagaacctt gaaccagagc gggataaact attaattcca
    11821 caggatagcg tcgttttatt atgtttatta tatcctgaag cgctgcacct gtaggagaag
    11881 tgattattcc aatccggtca ggatatttgg gaaggggctt tttatgctct gctgcaaaaa
    11941 gtccttcctt ttccagtttc tttttcagct gttcaaattt ctgctgtaaa agtcccattc
    12001 ctgcttcttg catattttgc acattcagat tataataacc gcctttttcg taaacagtta
    12061 gcttgccgaa acagataacc tgcattccat cagcgggttg aaaagttaaa gaagcattgg
    12121 cacccttgaa aaaagtgcag cgtaaggtgg cattttcatc cttcaggtta aaatagatat
    12181 gaccagaact atgacgaata aaaccagaaa tttcacccat tacaaatacc ggttgcaggg
    12241 atgtttcaat cacctgacgc agctggcggg ttatttcata aacgctatag attattacat
    12301 tttccatttc aatgccaaaa tctaaacata acgctatttg tcaattctaa taccatcttt
    12361 ttcttgatga gtcctttatg tcagaaaaaa ttatttcctt ttggaaaatn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctttttctt gatgagtcct ttatgtcaga
    12541 aaaaattatt tccttttgga aaatactatt attgttaaaa cctctatgga tttctgattt
    12601 tacaggaata gcaaaaaata tatttctgct ttagcaaaaa tgacatttga atggtagata
    12661 ttaacaatgg gtttcaaccc ggtgaaaagt gaaatataaa acatcttatc tcttttttta
    12721 agaaagagtt ccgtaggaac gacagatact ataaaagggt ttcaaccctg agaagaaaag
    12781 tcaaatttgg cgttttgttc gctgaattca gattagttat aaattaagaa catatatttt
    12841 tcagagttct gcgaactctt tggcaaatta taatttatca acccattttt ctacaactga
    12901 agattataat tttagtaatt gataatcaaa tctctgattt tgcgtttcgg aacataatga
    12961 ataccttttt catcacgcca atattcaatt tttccctgtt ctgtaacatc ttctataaca
    13021 actgattctg caggatagcc caaagcaata accagcataa tttccatctc ttcgggcaaa
    13081 gaaaaggtat tttgaacttc ctgtttatca acggaagcca gcatacatcc gccaaaacca
    13141 ttatccacag cgcttaagag tattgtctga gcagcaattc cggtatcaat aaacaccatc
    13201 tttgggctat caaccggcgc taaaataata atataagcag agggtctttc tcctttttca
    13261 ggtccattcc agttttttaa ataacttgcc cacttcaaat ggggaaaaat caaatcgcat
    13321 ttatcctctt cataaataag gaaatagcgc aatttttgca gattacccgc actgggaatg
    13381 tatctggcta cctttattag ctctataagt tgtgcgcgag ttaacctctt ttcttcgtga
    13441 aacctgcgat aactccgatt gcgataaacc aggtcaaaaa tcatctttct ctccttaaaa
    13501 aaagttctat ttctgtgtct atatataatt ataatgaata cttgcacatt tgaccagaca
    13561 tttttagtga cagtaatata aatattagat tgagaatgta taaatattta tttatctcat
    13621 tttgattcct gctttcgcaa gaatgacaaa tggcttacta ttcaaaaatg atatcagctg
    13681 ttgtaaccaa aaatcaaccc ccaaattaac agcaagtttt acgcaaattc agatttggga
    13741 agaaatgaca tctattatat atatctgctt gttaatcaat tgattatcta tcttgcctat
    13801 caatgttaaa ttccaacacc taacttattg ataattagtc agttctctaa cgactccgta
    13861 acacttccgt aacactgccg taatctcgtt acgggatcgt tacttggttg ttactggatt
    13921 attaaattgt tcagaaaggg aaaaaatatc gctcactgct ggcaaagcta taataggaaa
    13981 gcaacttaaa cggattttaa caaattcaca cggatttttt tattatcagt ccatcttttt
    14041 tcaagacaac ccattcattt tctattcttc attacagcag tccccatctt cttcttaaaa
    14101 acagttccgc acagaaggtt aaaataaaca gggagagaat ataccacttt ttatagaggg
    14161 ggatttcttt gcgggaaatt tgttctacag gtttggcagg aacgggatta aaggtattta
    14221 gattgctttt gtataataat tttccacctg tttctttagc gagccaggaa agaagtgaca
    14281 aattatagcc ataatctctt gtttcaatag atgtctcgga gagttcaaat ttgcctttgg
    14341 tgctttttcc tgtttccttg tcagagattt caaagctgta agttcccggt tctgtaagtt
    14401 gaagagtgat agcatattca tcaccttcgc gtttcagaaa atcggtatag acctctttat
    14461 tatttttatc cagaatacga atacgcgggt taacatccaa acgactttgg cgaatttcgt
    14521 cttcactacg cagacggata cgaatttcct caccccgaaa ataactgtta ttgtaaatag
    14581 cgttataagt tcctgtgctt ttattactaa gccaggttaa acaattactg attaattttg
    14641 tgtagccatc atctccgctt tgcatttgcc aacgccaaag gtttaaagtt gcaaaagcca
    14701 gagagcgtgc agttcctgta gtttgaacaa caattgccgg agaattttgc ggattattaa
    14761 tagtagctaa aatttcacta cccgggctgg cagttacata ataataatcc agcggtggaa
    14821 tatctttggc atttaaggtt aaactattca gcataggata gttagccgag ataggcgtag
    14881 gaactatgaa tccttgataa gtagaagtaa tattggattt ttgaattggc aaaagagaag
    14941 ctaattcgtt tacgggaagt ccctgataaa ggattcccac tccgcgatta taggcatttt
    15001 gtataaaact taccgtagca ttatccaaat tgagtattcc gttattgata ataattaagg
    15061 cagctaagtt atctggttca agtttattaa ctaacacttc tccgctataa agacgcccgt
    15121 ttctattcag atagctttct gctgtccaac ggctatttgt tgcaatagca tccagagtga
    15181 atttattatc ccaggcgggc ttatcggaaa taaggataat cctttgtttt tctgcgagca
    15241 cttcaatagc tccagggtag atattattgt taatttggct ttcatttggc aaaggggcaa
    15301 cttccacttt ccagggaaaa aaaccgatgc tgttaaactt atgggtaaaa tccacattag
    15361 cggttatgcc tttttccagt ttcaagtttt ggcgtgagat aattttattt gccatcaaca
    15421 aacggacttc tgcaggtccg ctgtaatttt gagaggaaat ttcagcacga atagtattgg
    15481 gctcatttct ataggcataa cggttacaac gcacattcac tattgccaaa tcgggagcag
    15541 gattttgaga actatcggct aaaacataaa aaggacagcc caattgctga actgcagata
    15601 aagattcatc tcgcaaccag ccatctgaag caagaactac tccttccaaa ttggtaaata
    15661 ctttttgacg actaagttct tttaaggcag gagacaataa agtgttattt ttatctcctt
    15721 ccaagccatt agcaaaggtt ttgtgaagaa cttcatagcc gctagcggta aatttctttt
    15781 ccatttcctg ccaaagtggt tttaaatgct gagatttggt tgtggaatta tgctttaagt
    15841 ccatactttg agaaatatct tccaatacca gtatttgagg tgcctgcttt ttgtgttgag
    15901 tgtaatataa aatggggctc atcaacaata tgagtagcag gaaaagtgta ataaacctta
    15961 aggcaaaaag aatccatcgc cttgatttgg taatttcggg attagttcta tagttcagaa
    16021 aacctgaaaa taaaagtgca aacagaacta tcagaacata tttcagcatt atagatgaac
    16081 cttcatataa cttcctatgg taattccttt ggttttgtaa ccaggaataa gatttttaac
    16141 actttgcaag ccaaaaccga attctactgc tttgctgcta tatccgattc cataacttat
    16201 gcgccaggga taatgactgt tgccaaaacc cacaccaata ttcagaggta aatattccca
    16261 gggcaaaaat tgagcagcta aagaaaatct gccaattccg ctggtagcaa tagaagtttt
    16321 aaaaccctga agataatcta aggaaaaatt agcccaaggt attttatata gagtgcccaa
    16381 acgtaattcc ggaggaagct ttgtagaata agaatcaata tctacagttt catttgcgta
    16441 atcataaatt tcctcggaga tattagccgc ataaacatca ttggcaacca ctttgatagt
    16501 tgtttcttca gtagttagaa accagtttat tttgccaaag aggttatcta aagtcaaccc
    16561 tacttccaaa ttgggagtga tattacttgc caatcccaac attgcttttt gcccgaaacc
    16621 ccccagacct gtatgtaaaa cgatatcctg tttaacatcc acaccctcca ttgaagaact
    16681 gaaatagccg aaaaaatcgt ccagattggc atttcctatt cctactaagg cactaacgga
    16741 aaagccagct ctaatggggg gaatagtttt cggaatatag ggaagagtaa aatttcccat
    16801 tgcaaaggtg atatcaacat aaccaagacc tccggcatcc gtagtgcttt tgtcaaatac
    16861 atacaaagaa tcggtattgc cataaagcag aaggtctaaa tacctttccg aaagagccag
    16921 tttacctgtt aaatgaagtg aactggaaag agacatatta tcaaaggcaa aacctacaat
    16981 actgatattg ccttctaaag ctgaacgcaa agaaccatca atcttattta ggattttctg
    17041 tttatctatg tcgttcaaag tatccctgct aactacatag ttataggtat ccaagtccaa
    17101 agagttatta tttatgccta ttcctaagtt cataatcggt atccagaaat caataccttt
    17161 ttcctgttgt aaattggcag gattccaata agcagcttca actcctttag cacgcagcat
    17221 ataactatcg gcaaaaaaga cggatttacc tgtattaaag gcattcaatg cagtcagaaa
    17281 cagaaggtag aagagtagta acaaagctaa ctttttcatt ttagtcctcc ttttcaatca
    17341 acacgcgggc actcatcatt cctttgatat gaatgtaatc catagtgctg gcgtgaatag
    17401 taacaggagt tcctgtctct tcaaagctga aagcgaaacg cataaaaacc tgctcggaag
    17461 taaaaacatt cagttcatct ttatttaagg tcaaattcac attttggaaa tccggttgta
    17521 aattagcact ataaattgcc gcttctttca taaagttgta agtgcttggg ttattagtat
    17581 ctattgaggg tgtagtgctg aagaataatt tggcagatgc tcctataggg agtttatttt
    17641 tgagttctaa atttaaggaa gcatttacta aattctttct aattcgctcc cggttttctt
    17701 ctggaatgct gaattcctgc tcttctgcta tagtaatttc gtgcgcgtgt aaagtaaaag
    17761 ttaagggtgc acttacagtg taaaataaaa gcatagtatc cgtaggtctt aaactgccta
    17821 tatttgttcc actgctaatt aaaaactgtc catcaataac ttgaattgta gtaggcataa
    17881 tctgtaaaag ctgtgccaca ttatgcccga actggatttc aacaattctg ggaccttctt
    17941 ccgtagcagg ttctatttga taattattac catattcatc taagatatca ataatcctac
    18001 tttgccctga ggaatttgtt gcctttagct gtccggaaaa aacagaaccg aaacctaaat
    18061 agttctgcaa agtaatttgc aaataggcat cttccaggtt aattgcctga tcaagtccat
    18121 aaggataatc aatgttaacc aaagaacctg aatcttccag tccaatagga taattattta
    18181 gagttcctct gaattctccg aactgaattg tttcgttggc ttgaaaatca aaggtggcta
    18241 cttgcattcc ggaaggcaga gaaggtgtaa tctgaaagga aagatgtatt tgattaataa
    18301 cttgatctga atttgccact cctaaatgca aaccttccaa atttgtggtt acccagtttc
    18361 ctgggttatt ggaagtaaga acaagatttt ggttattagg caaataaagg tcataaaaag
    18421 ttaaggtgat ttcaacaccg gattgcaaat tattgaaacg acttttaaaa aaaccttccg
    18481 ctatttcagc ataagcaaca ccaactttac cgttttggtc ttcaaaagga attgtaagat
    18541 caagcccagt actccaaatg ggaagtccct caacattaac atccggtgta aaaggtaagg
    18601 tgccaaattc gtttgtagtt gcttctccac tggtagttac tgtcaagacc tcattttcac
    18661 ccataacgat attaaccgaa tccaccagat cggacacata gtatctttcg ttgatgatag
    18721 ggatattcaa atccataaac caagtgggca tcgtaggttt ttttatttcg caggcggtta
    18781 aaagaatcaa ggcaattata agcataagat agattttttt cataaacacc tccttccttt
    18841 gtgtttagga ctggtcttct gcttttgcta aattaagaac cggcttatag ataatgttat
    18901 ctataccaga attgacactc tatatattgt ataaaaaaag aaatacactt tttttcttct
    18961 tccacttaaa aaagcttttt agataaaata agcatttgtt ctcttatggc aaattttata
    19021 ctcgtatcct tgttatattc attctttatt tttccttaat gtcgtcataa gtcctcactg
    19081 caaacaaatt tgagggttgt gtaaaagtta tataggtggt aacgactcct tacttactaa
    19141 attcctaatt tttggcaagc catccttgcg gcttcctgat gtgcattttt cttggtattg
    19201 cctgttcccg ttcctaaaag tttgtttccc caacggactt ccactacaaa tgtcttatga
    19261 tgttcagggc cttgttctgc aacagtaaca taagtaggtg gcggttgatt tctgctttgc
    19321 atatattcct gtaagatact tttatagttc acaagttctt ctctggtaac cgtttcctga
    19381 taattattca gaatatgatt acggataaat ctggtggcag cagaaattcc actatccaga
    19441 tagatagcac aaataagggc ttccatagca tccgaaagaa tagagggttt ggatgcacca
    19501 cccgattgct gttcttcagg gcttaggagt aaatattttc ccaggtctat agcttttgct
    19561 tttaaggtaa gataggtctc ggagacaatt ttggatttca gcttggaaag ttttccttcc
    19621 tgttcatcag gatgacgggt aaaaagttct ttactaacga taaaaccaag aatggaatca
    19681 cctaaaaatt ccatccgctc aaaaggagaa ggagttttat ggtcttcaaa atgacgatgc
    19741 agatatgact tatgagtaag agcagcatat aataaagtgg gatcatgaaa ggtataatca
    19801 atcttttttt gtaattggga aagcgatttt tcccatttag gatactgctc cgttatcttt
    19861 ttggcattga aataatccag aatacgggag attattgatt tcatccgttt ctcaagcgtt
    19921 ataaaactcc atcttttatc aagcgtagcg tttgatgatt attgcggaat tatggcctcc
    19981 aaaacccaaa gaattggaaa gtgctatatt cacaatatgt ttaacagctt gattgggagt
    20041 ataatccaaa tcgcattccg gatcgggatg agttaaattt atggtaggat gaatgatacc
    20101 tgtttcaatg ctcttaatgc aaactatagc ttcaattccg gcagcagcac ctaacatatg
    20161 acctaccata cttttagtgg aattaatctt cacttggtaa gcataatcac cgaagacatt
    20221 tttaatggaa atggtttcac ctttatcgtt taagggagta gaagttccgt gtgcattgat
    20281 gtaatcaata tcttttggct taagtccggc atcctgaatt gccatcagca ttgctctgga
    20341 actgccttct ccatcttccg tgggagcagt aatatgaaaa gcatcttcag aagcaccgta
    20401 accaactaat tcggcataaa ttttagcgcc tctggctaaa gcatgttcca gttcttccaa
    20461 aacaagaatg gcagcacctt cactcattac aaaaccatcg cgttcagcat caaaaggacg
    20521 cgatgccttt tggggttcgt tattacgcgt agaaagagct cgcatagcag aaaaaccacc
    20581 taccgacaaa ggagttacag ctgcttcagt tccaccgcta agaataatat ctgcgtcgcc
    20641 atattgaata gaccgaaaag cggttcctaa agcatgattg gcactggcac aagcactcat
    20701 aacattaaaa ttgatacctt taaaattatt ttctatgctg atatgggcag ctgcaatgtt
    20761 gccaatcatt ttaggaataa agaaaggact gatacgacgc ggtccttggg tgtaattttt
    20821 aatgcattct tcttcaaagg taagaatacc tccaattcct gcaccggtaa taactccggt
    20881 tctatcggga ttaaaagaac caggtattaa accagcatca gcaattgctt ccctggcagc
    20941 aatcatcgca aactgagtgt ataaatcaag ctttttaacc tctttatggt caaagtgctc
    21001 ttcgggatcg taatttttta cttcggcagc aatgtgaacc ggtaaattac tgtaatcaaa
    21061 tttggaaatt aaatccactc ctgagacgcc gttaactaat gactcccagg tttgagtaac
    21121 attatgtcct acaggagtga tagctcctat accggtgata acaactcttc gtttattcat
    21181 aaagacctca ttatttggtt ttacttaccc ctttactctg aattttatct ccatctcctt
    21241 tcaactggct ttgtagtgag gaacaagata aacttccgta aagggtggat acaatcctga
    21301 tacttatgca gttatcagga ttgttccaca ttagaggaaa gggtatatta tcccagtttt
    21361 tccttcaagt agtcaattgc ttgaccaaca gtgcgaagtt tgtcctgatc ttcatccgga
    21421 atcgtaattc cgaattcttc ttcaaatgcc ataaccagtt caactgtatc taaagaatcg
    21481 gcacgcaggt cttcaatgaa attagcggaa ggaacaacct gggattcttc aactcccagt
    21541 ttatccacta ctatctgttt tactttggct tcaatatcca ttattcctcc tatgttattg
    21601 aagtgtttct ttatctttgt tttcgttcaa tgcattgtta agccaccatc aactgcgatg
    21661 gtttggcctg ttatatagct tcctgcatcg gaagcaagaa agagacaaac attggctaca
    21721 tctttgggag tgcccatttt ttgtaaagga atggctttgg catattcggc tctgatctca
    21781 ggacttaagg atgctgtcat ctctgtttca ataaaaccag gagctacagc atttacgcgt
    21841 atattgcgac taccgaattc cttggcacag cttttagtga aggcaatcag tccacccttg
    21901 gaagcagcat agtttgcctg accactattt cctgtaatgc ctattacact ggcgatattg
    21961 ataatacagc cattacgatt acgcatcata tatttaaatg ccttttgagt gcaaataaag
    22021 ctgcctttca gatttacatc catcaccatt tgccagtcct cttccttcat acggatgagc
    22081 aaattatcgc gcgtaatacc tgcattgttt ataaggcaat caatagttcc atatttatca
    22141 acaatatcgg cgaagatatt ttcaatgttt acgctatcag ttacatttcc tacatagcca
    22201 taaacctgaa accctttggt ctttaattct gcaacggctt tctctacggt ttcagcagaa
    22261 atatcaataa ttattacttt agctttggct tctccaaaag cagtagctag ggaaaaacca
    22321 atacctcttc ctgcaccagt aattactatt actttattag aaaaatccca gttcattatt
    22381 tccccattag tataattttt atttttagca acttaacttg tagctgacac cacaccatca
    22441 atgtcttcat atttgtcaat tgaaaaaact tccacatcct tattgatgtt tctaatcata
    22501 ccgcttaaga ccttttgcgg accgaattct ataaagcgtt gaaccccttg gtcaatcata
    22561 tactgcacac tttgaaccca taaaacaggt gaaacaattt gtcgggcaag gagttctctt
    22621 tcttcctgtc cattcataac tggtagagca gtcacattgg aaattacagg tatttttcca
    22681 tctttaaaat ccactttctg catttcttcc ataagccaat ccttggcttt gtctattaaa
    22741 ggagtatgaa aaggaccgcc aactaccaaa ggaataactc ttttagctcc tttggctttg
    22801 gcaatttcac ccgctttttg aactccttct gcggttccag aaatcacggt ttgaatagga
    22861 gtgttaaaat ttacagctct tactaaaccg acactttctg cttcagaaca gatttctttt
    22921 accaaatttg gctctaaacc gataatagca ctcattgcaa aagggacacc ggcattggct
    22981 ttaatcataa attcacctct tttatgcacc aaatgtaagg catcctgaaa ggtaatcatt
    23041 tctgaagcga caagggcact gaattctcct aaagagtggc ctgctacaaa atctgcctgc
    23101 agtttacttt tattttgaaa cccgtaaaga gcggaaatac tgtgaaataa aatagcgggt
    23161 tgagtataac ttgtctgttt tagtgcctct tctggtcctg aagccattac ttcagccaaa
    23221 tttgtgttat gagctttatc aaagttatcc agaatttcct tcaaagcagg gtctttttga
    23281 ataaagtcat ttgccattcc tacatactga gaaccctgac cgggaaaaac aaaagctgtt
    23341 ttcattattt acctcttttg tgattggagc ccgctttagc ttcgcatctt tttttgcgga
    23401 gaaacagcgt tctctgatta cacattggtt gacaaagatg tcaaccttcc gttattaata
    23461 tcttgctaaa atacttcccc aggttaatcc tgctccaaag gaagtaagca aaataatatc
    23521 accgcgtctg atttttttgt ttcggattgc ctcatccgct gcgataggaa tcgtagcaga
    23581 ggaggtattc ccatattttt caatattcac aataacctta ctcataggca ctttcatttt
    23641 ggaagccagt ccttcaataa tgcgtaaatt tgcctgatgg ggaatgatcc aatccacatc
    23701 tgcagtagta agattattgc gtttcaagag ttcatccgaa gaagcataca tacatctgat
    23761 ggcatttttg aaaatccgat tgccttccat ataaacagta tgcaggtttt tatccactgt
    23821 ttcgtggcta gctggcatac gcgaaccgcc tgcatattga attaaatact caccctgact
    23881 gccatctgca gctattttac tgtctataat acgcgaaatt tcgctgggtt cagctctact
    23941 tacaattgct gctccggctc catcaccaaa aagaacgcag gtattccggt ctttccagtt
    24001 ggtaattttg gtaagaattt ctacacctat aaccaaaata ttttgcacaa ttccattttc
    24061 tacatattgc cgagctacat ccaaagcata aataaaaccc gtgcaaccgg cagaaatatc
    24121 aaagcaagga atatctttca gccccaattt cttttgcaaa atgcaggcag ttgaagggaa
    24181 aggatggtct cctgaaatgg tagctacaat tatttgatca atatctttat atttaacgga
    24241 agaactttct attgctctaa cagctgcttc ataagctaaa tcactggctg cttcattttc
    24301 ggaagcaata tgtctttcaa acattcctgt gcgggttcta atccactcat cgctggtttc
    24361 cacaatcttt tctaaatcat aattattcaa tatcttctgg ggaacacagc taccaaagga
    24421 ggataatttg gcataataat tcagcataat taagacctct caaaatagtt tctggcattt
    24481 gttacgaagc cggatttggc aattaaggca gcaaaacgga tagcattttt cattgctttg
    24541 gcattactgc gtccatgagc aactacagaa attccgttta agccaacaag taaagcacca
    24601 ccgtattctg tatggtccag tttatttttt atatgggtat aaacaggata ggaaagaagg
    24661 gcaccaagtt tggcaatcca gtccttattt atctgttctt tcaatatctc aaaaatagaa
    24721 aagcccacac cttcaatagt ttttaataca atattaccga caaatccatc gcagacaact
    24781 acatcggtaa ttcctttaat aatgtccttg ccctctatgt taccgataaa attaagttct
    24841 ttttcctggc tgagtaattg atatgcctgc ttggttgtag catttccttt ggcactttct
    24901 tctccgatat tcaagagtga aattcgggga tgctcttcct gaaaaaaata cttgaaatac
    24961 atacttccca gtttggcaaa ctgcactaaa ttttctgctg tgcaatcaac attggcaccc
    25021 atatccaaaa gaatttgatg tcccgtttcc gtagggaaaa caacggcaat agcaggacgc
    25081 aacacatttt ttatgcgacc atagattaaa agcgaagcag acattactgc tcctgtattt
    25141 cctgcgctaa cagcagcatc tgcaagacct tgattatgaa gctcaattgt tttcaccaaa
    25201 gaagaatctc ttttagaacg cacagctgtt gcaccactat cttccatttc tattctctgt
    25261 gtggcattaa caacgctgat tcttttttgc ggataaaaga atttttccag ttcgtgtttg
    25321 attttttctt catctccaac caggatcact tcttcgcaaa ggtcttcttt gatagctaga
    25381 acagcgcctt caatttccgg aaacggtgca ttatcagttc cgaaagcatc tacggcaata
    25441 cgcaccttac tccttaactg ttaatatttg cttttcatta taggtgccgc atttttcaca
    25501 aatatgatgc ggtagtttgg gttctccaca tttcttgcaa gtgcttatag ttggaggagt
    25561 taagacctta tgagttctac gtttatctct cctgcttttg gaggttcgtc ttttggggac
    25621 tgccatttaa tttctcctta tcttttgata atttttttta gcaagacaca agcaggaaca
    25681 tattatcaga ggtggaaata atccttcttt ataaacaatc gcttgtgctt tctaagcatt
    25741 tatgaactga acttaaaagg gacatttttt gtcaatcaaa atctcttttt ggggagtaga
    25801 cgaaggacgc tgttcttcaa caagttccaa aaaattagaa agatggcaag ctataattac
    25861 aattttgttg aatatagcta tcttagcaga aaatcttact tcttatcttt tttaacctat
    25921 aatgacaaag taacaaccaa gtaacaactc cgtaacgaga caacggaagt gttacggaag
    25981 tgttacggag tcgttactta atcaactata taacaagcaa ttacgcattt caaggcaaca
    26041 gggacagaga atagtgttaa ccttctatat tatcaataag atatgtaatt gtaattcgct
    26101 tttacccaaa agaaacttgg caccccgatg cccaagaaga atatttttta tttaaaactt
    26161 cttggctgga atgattattg ctttattttt aagcattatg aaaagaatta cagttcttct
    26221 tctcggtcta tttttggcgt gtaatttttt tgccatagcc aggcaagata gttccaaaca
    26281 gcaaccgctt tacagcgcta atgttgtgaa aataaagctt agccaggatg cagttaatcg
    26341 ctcccaattg ccaattaatg cttatgaaac aagggaaaag accaatttta acgaacttga
    26401 tcaattattt gccttaaacg gaataaagtc catcacccgt gctcatattg ctgccaaaga
    26461 ccaaaaatgg gttcaagata ctggttttga tcgctggttt ttagttcact taaatggaat
    26521 taaaagcgtg gaagaagtaa ttgccaatct aaaaaataag cgctatattg aagaagcgat
    26581 tccggaatat tacgcttatt tgcataatgt tcccaatgat ccttattttg ctaataattg
    26641 gggtcataat aatactgctc agcttcctgt ttatatacac ggttctcata gcggacctgg
    26701 ggttggagtt atcggttttg acagcgatgc tattgcggcg tggggagaaa cagatttctt
    26761 tggagacccg aatattatta tagggatttt ggatacaggt gtagatatta atcatcctga
    26821 tttgcgtttg gttcagggct atgattttgg ggataacgac aatgatccga atgataatag
    26881 tatgaacgca gggcatggaa catgcacttc gggaattgct gctgccatag ctaataatgg
    26941 aattggggtt tccggaattg ccggcggctg tagtgttatg cctataaaag tatctaatag
    27001 tcatggtgaa atatcattta cttatgctgc taatgcctta atttatgctg cagaccaaaa
    27061 tgtggatgta gtcagtatga gttttggggc ttatggtatg gaaataggtt ccgatcctgc
    27121 cgctgatagt gctttgagtt atgcttataa taatggagta gttctctttg ccagtacggg
    27181 aaataatgat gagccggcta tagcttatcc ggcaaaacat cctgctgtaa tagcagttgg
    27241 agctgccagt ccttccggag aaagaaaaaa ctccaattct tccgatggtg aatattggtg
    27301 gggttccggt tactctttta tggaacagga tacagcagga gcggttgata ttttggctcc
    27361 tactatttta ccgacaacgg atattcaagg ttctggcggt tatactgatg ctaattatta
    27421 tatgtggttt tccggaacat cctgtgccag tccttatgca gcaggagttg ccgctttagt
    27481 tcgttccgta aatcctggtc tttcccctgc tgaagtaaga aatataatta cttccacagc
    27541 cacagatatg actattaata cttttgaagg ttgggattac tggacaggtt atggattggt
    27601 aaatgcctat caggcggttt tagctgccaa tccttattta ccgcaatgta aaattgtcta
    27661 tcccattaat aataccggat ttgtaatcaa ttcgctcatt cctgttcggg ttgaggcatc
    27721 tgatttggac gatagaagta taacggaagt ccgtttcttt ctggataaca atcctgatta
    27781 tgttttttcc gatgctacaa tgccttatga atggatttgg aataccgccg aggcaacttt
    27841 ggggatgcac aaaataaagg tagtagtaat tgataatgac ggtaatcaaa gacaggatca
    27901 aatagttata tctattgtct ctcctgccga tgaaggtttt gaaacaggtg acttttcttc
    27961 attgttttgg aataacaaca gtcctcaacc ctggtatgtg caggatgata tttattttac
    28021 gggtgccttt tctgcccgca gtggagatgt ggaaaacggt tcattatccg aaatgaagct
    28081 gaaaataaat gttactcaac cgggagatat cagtttttat agaaaggtat ctgcccgaaa
    28141 agaatttagt tacctgcgtt tttacattga tgataatctt gctgttcaat ggacaggaaa
    28201 aagagattgg gaactggttt cctgtccgat agagcccggt ttgcatactt tttcttggac
    28261 ttattacagt tacgaaggta gttatgaata tgataactgt gcttggatag accatttgat
    28321 tttgcctgaa tatgaaaact actattggcc tccgcgaaat ttgacagctg ccgggggaaa
    28381 tggctttatt cttatttact ggcaagttcc ggaagcagga aatcctatcg gctataaaat
    28441 ttaccgtaac gatgttttac taactacaat tacgggaaca agatataccg atacggatgt
    28501 aacaatcgga gttccttatc aatataaaat tgttgccgtt tatgctgatg gtgaatcgga
    28561 atcaacttcc ccccaaactg caactcctat agcggaagat gttacggaag caattattgg
    28621 aacaggaacc actgctaccg gttctgcaga gggctgtcct attaataatc ttcggaaaag
    28681 tttgcacggt cagatgattt ataaagccag cgaattaaat ctgcaaggta tttttggtcc
    28741 catatatatt tcccgtttgg gctttaatat cgtttcttcg cctttatatt cacttcctgc
    28801 tttccaaata agaatggctc atacagccgc agaaaatgtt tctcaatggc gaggtgaaga
    28861 aaacttaatt acttattatt ttgctgataa ttacagacct gttccgggtg gttttgaaat
    28921 gcttacttta gatacccctt ttcgttggaa cggaatagat aatattttga tagatactgc
    28981 ttttagcccg gcaaatgcaa cttcctctta tggaacagtt tgctacacag taacccctaa
    29041 tggttatcgt tacacccgca gtgatactaa cgatcaaacc gatatttttt atggcggaac
    29101 tttaactgtt tacagaccta acctgaaaat atcaacggct aatatgccaa atattcaaat
    29161 cagtgctaac aacctggttt ttggcaatgt tcttgttggc tttgaaacaa gcaaatcatt
    29221 taccataact aacaccggaa cctccctgct aacgggagaa attactgtgc ctaatggctt
    29281 tagcattgcc tgtaggggaa agaaacaaaa taccgtattc ttctctattt ctgctgataa
    29341 ttctgctgtt tttaccgtct tttttactcc tgaatcgcca gggacttata atggcaattt
    29401 aattattact cataatgttg atagcgaaaa taggattata gttctgaagg caatttcgct
    29461 aactgcccaa aatactccct ttattgacgg ttttgaaaca gatagctgtc agtggacttt
    29521 tgccaatgaa aatcagacca ataaatggat gcgaggttct ctcgttactc aagccggaag
    29581 aggaggactt tttatcagtg gtgatggcgg aatcagccat acttatataa ccaatagtgc
    29641 ttctgtcagt tatgcttaca aagatatttt aattcctgag ggaaccgaga attgcaaatt
    29701 acgcttttcc tggaaagcca aaggagaagg aacaggaccc tatattgact acctaagggt
    29761 ttattgtgtt gaacccgatg tagttccgga agcgggaatt gttttaagca caggacaatt
    29821 gagctctcct ttgaatcata cggaaatttg gaaagaagca gaccttaata ttccgaattc
    29881 tttatcgggc ggacctaaaa gaattatttt gatgtggaca aacgatgcta ctgggggaac
    29941 tcaacctcct gctgcagttg ataatattcg tgttcttctc aataactata cggactatgc
    30001 ctttgtttac aatgggttgg ctttagttaa tgtcccggct gtatccgata ctttaggcaa
    30061 tacatattat cccaaactta caattgaagg aattactaat ccggaaaatt atttaactgt
    30121 tacttccggt tttgcctcgt tagatgagcc ctttgcggat gcgggaatgg attttactct
    30181 tacaggagcg aattttagcg gcgcagaaat tattttttat cataatttag gatttacacc
    30241 ctattatctg ggctataaaa ttgaaggcag aggaatatgg atgattcaat cggcagaaat
    30301 aaactggaca aatgaatatg cttattttac tgtgcctgat ttttatccca atgccaccaa
    30361 aatatcattc tgttttcccc gtcagaataa tgttttgcca ccctatctaa atagttttac
    30421 ggcaaccctg aacgagagcc ataaagttgt tctttcctgg ataagcaatg cggaaacagg
    30481 aatatccgct tttcaaattt ggcgcggaaa tagcagtaat ctttcttctg caatagctat
    30541 cagcgattta atacccttta atcctgccca acaaaataat tatacttata cggatacaac
    30601 ttctgcagct cttacccgtt attattactg gctgaaagtt atcaataatg atgctgtaga
    30661 gaatttttgg ggtcccatta caattttaac ttccaatgat ccacccccgc ttcctcctcc
    30721 ggaaacagct ctattaggag tttttcccaa tccttttaat cctggcacaa aaatcaacta
    30781 tgccctgaaa caggaaggaa ctgtggaatt taaaatctat aatgctaaag gacaattagt
    30841 aaatagcttt tctgaaactc attcttctcc aggaacctat gtttgcgagt tttcgggtaa
    30901 aaacaaagag ggcaaggaat tagcaagcgg actctatatc tgctctatgc gatttaaggg
    30961 gaaaacctat gtccgcaaaa tggtaaaaac caaataaaca tcaacagagc tcataaaaaa
    31021 gttccgttgg aactttttca tcttggtcca atggttatta tgggataaaa tataattttt
    31081 atatctaact aaatacgctt tagcaaacta caaagtagag gagcttggaa ttatataaac
    31141 attgctttaa tctgccaatt attcaacggt cttttataat agtgtgagtg aggaggataa
    31201 atcctgccaa ccaatgtgct tgcagatatc attttcccag gtttgatttc ctccataaac
    31261 aattctggct ttccctaagg gcagattaga tttgcttaat ttcaggatat tgcgatagaa
    31321 ggaattgtta attacagagg cagattttat ttccagaggg tagattagat ttcctttttc
    31381 tattaaaaga tcaatttccg ttccatttga ttctctgtaa aagcttaaag gaggttggat
    31441 acagcggttg tggaagaatt tcagcacttc ggaaagaata aaggtctcaa aaagattccc
    31501 gcgtaaagga tgatttgcca aatctgtttc attgcgtatt tgtaacaggt tgcaagcaag
    31561 tcccgtatcg taaaaataca gcttggggct tttaatcagg cgtttagata tgtttttttg
    31621 ccagggctta agtaaataga ttatatagct tgtttgcaac acggaaagcc aattaccaat
    31681 tgttttacta tctaccccta tctcatttgc cagagattcc ttatttaata gagagccagt
    31741 tcttcctgca catagagaaa gaaaacgggt aaagtcatcc aaattttgga tattgcttag
    31801 taggcggata tctctttgca ggtaagttct aatataggaa tcataaaaga cagtaggttc
    31861 aatattatga acaagtaaac gcggataaaa tcctcgaaaa atacaagtat taagagattg
    31921 tttttccttt ggatatgctt cttgcaaact gaaaggcaaa agttccatta tggcagacct
    31981 gccggctaag gactgtgaaa tatccttcat taaagtaaat tgattggaac cggttaagat
    32041 aaactgtgtt tccttttgga cttcatcact aatacttttt attgtagata acagctccgg
    32101 agcatattga aattcatcta cgatataatc tgcttgttta tttgcaaata gagaggaggg
    32161 gtcttctttt gctgctcttc tggtatttag gttttccaaa tcaagataat agtaatcggg
    32221 aaaagtcatc tttgccaaag ttgttttgcc tgattgtctt ggtcctgtaa tagttatgat
    32281 aggaaatcct ttacacagat gaagtaaatg gtcttgcatt tctctcttaa ccatctttcg
    32341 tctcccattt atgtaaattt ggaatctaat tccatatttg cataaaatta gggtcgggaa
    32401 aagctgtcaa ctaaaacttt tcttacaccg aaaacgccga gaggagatta aagtgaagaa
    32461 tgaaaagtga tcataagagg ggaggtcaga aaaaataaat aataattgat tttttagaac
    32521 tacttatcat agacccgatt attaaaatga tattggcgtg ccttctttca atttgtaaga
    32581 aacaaaccag cttttacttg acgccaaacc ttcttttcaa aattggttca aaacagttaa
    32641 aaaatatgtt aagaagaaca aaacaacgac aaaaaaagag gtaactgcca aaatggaaat
    32701 aagtaagaca tttgaaccta aagacctgga aaagaaatgg tatcagtttt gggaagaaaa
    32761 cggctatttc aagccccgcg aaaacactgc ccaaaaaccg tttaccgtcc taatgccccc
    32821 tccgaatgtg acaggtattt tgcatatcgg tcatgttttg aataatactc ttcaagatgt
    32881 cgttgttcgt tatcaccgaa tgaaaggtga acctactctt tggctaccag gagttgatca
    32941 tgccggaatc gccacgcaaa atatggtaga aaaagaactt gccaaacaag gaattacgcg
    33001 tcaggaatta ggtcgcgaaa aaatggtgga gaaaatctgg cagtggaaaa atgagaaagg
    33061 caatattatt attgatcagc tcaaactctt gggagcaagt tgcgattggg atcgcttacg
    33121 ttttacgatg gatgaaatgc tttcgcgagc tgtgaaagag gtttttgtaa ccctatatga
    33181 agaaggactg atttataaag gcaaatacat tatcaattgg tgtccgcgtt gtgttactgc
    33241 cttggcaaat gacgaagtgg aacacaccga tgaaaacggt catctttggt atattcatta
    33301 tccttttgct gaaggggatg gctatgttac tgtagcaaca acccgtccgg aaacaatgtt
    33361 aggtgatgtt gctgtagctg taaaccctaa agatgaacgc tacagttcct taatcggtaa
    33421 agaactgatt ttgcctttaa cggacagaaa gataccgcta ataactgatg aatatgtaga
    33481 tagggatttt ggaaccggtt gcgtaaaagt tactcctgct cacgatccca atgattttga
    33541 aattggcatc aggcataatt taccccagat tttagtgatg gatgaaaccg gaattatgaa
    33601 cgcaaatgca ggaaaggaat ttcagggttt agaccgctat gcctgccggg aaaaagtgct
    33661 taaaatgctt accgaacaag gtctgctgga aaaaacggaa atacattctt attccgtagg
    33721 gcgttgttat cgctgtgata cggttattga accctatctt tccgatcagt ggtttgtaaa
    33781 aatggctcct ttagccgaaa aagccataga agttgtggaa aaaggagaag ttaagtttca
    33841 accggaacgc tggacaaagg tctatatgca ttggatgaat aatattcggg attggtgtat
    33901 ttcgcgtcaa atttggtggg gacatcgcat tcctgtatat tattgtgatc attgttctta
    33961 tatgattgta gctaaagaga tgccagaaaa atgtcctaaa tgcggaggaa ctaaatttac
    34021 tcaggattcc gatgtgctgg atacctggtt ttccagttgg ttatggccct tttctacaat
    34081 gggttggcct gatgaaacag ctgatttgaa acgctatctt cctaccaatg tattaattac
    34141 agctccagaa attatatacc tgtgggtagc cagaatgata atgagtaccc tgcattttgt
    34201 agataaaatt ccttttgata ctgttctatt gcatggaact gtaagagatg aaatcggacg
    34261 caaaatgagc aagtccttag gcaattctcc cgacccaatt gatttaatta ataccgttgg
    34321 agccgatgct ttaaggttta gtatgatttt tggcacaccc aaaggtgcgg atgtaatctt
    34381 cagtgaaagt attttggaaa cgggacgcaa ctttgccaat aaaatgtgga atgcctatag
    34441 gttcattatg atgaatgcgg aaaatattga aggattacct gcggaaaatg aattgcaact
    34501 ggaacttgcc gataaatgga taatttcccg tttgcaagaa gtgataattg cagtgtgtgc
    34561 tcactatgaa aatttgcgct ttaacgatgc tgctacagaa atctttaagt ttatgtggga
    34621 tgaattttgt agctggtata ttgaacttag caaagaccgt ttaaatcctt cctcagcact
    34681 ttcgggcaga ttaacggcta agtatatact tttgtatgtg atgcaaactg gaatgcgtct
    34741 gttgcatcct attatgccat ttattacgga agaaatttgg caggggataa aaaaaatctt
    34801 tccaatggaa gaagaagcac tcattattgc cactttccca gaaacagatg agacacttat
    34861 agataacgat attgataata atatgcgctt tgtgcaggaa agcattactg ccattcggaa
    34921 cctgcggaaa caaataaacc tggctccaaa tcaggaaata gatatcgtta ttcggtttgc
    34981 tgaaaattct cagcagaaac tctttgaaaa ttatatgact tactttaacc gattggcaaa
    35041 agtgaaaacc ctttcagggg gagtaaatat tgccaaacct aaagctgcaa ttgcttctgt
    35101 ggttcgcaat attgaaatct ttttgccctt gagcggattg gtggatattg aaagcgaaaa
    35161 aaatcggctc ggaaaacaaa ttgaaaaact aaccaaagaa atgagtggaa ttaaggcaaa
    35221 acttgctaat cccaatttcc tgaataatgc caaagaagaa gttgttgcta aagagaaaga
    35281 gaaatttata gaagttaata ccaaactgga attgctgaag aacctgcttt ctgagttata
    35341 gacatattgt acacagagaa cacagaaaaa aagataagtg cacgcagatt acgctgattt
    35401 cgcagatttt tttacaccga gattatttta tagtttccga gttaatactc acagatagca
    35461 cagatttcac agagttacat aaattctcca ttctcagttc ttcctcaaat ctctttttct
    35521 cttttctctt tgtaaatttt ctcttttctc tttgttaatc ttcccttttt ctctttgtac
    35581 ttatatacat aactctttaa tattaaatcc tgttgatcct gtctatccag ttatctgcgt
    35641 tctatttttt taatttcttc tttttttctt cccatccaat tcctgcgacc aaaagaagaa
    35701 ccgttgccag aatacctacc aagcttaaag tcaaacttaa tttataggtt tcagagatga
    35761 atttcatttc cagagtatgt tttcccggag gaataattac ccctctcaaa atgtggttta
    35821 cagggtaaat ttctgttttt ttgccgtcta taaaagcttt ccagcccgct gggtaataga
    35881 cctcagaaac aactaaaaac gcatttttat cggtagctac atcatattta acattgtgca
    35941 gatcaaaata ggtctctttt gcaaagcaac tatccggttt aaaaacgcca tctatcttac
    36001 tttccacaat agctaaagag cgtggattaa aagacggttc ccgcattttt tgcaaacggg
    36061 cttcaatttc cggaattacg gatagggaat ctacaaacca ggcacgcggg taagcggtta
    36121 aattttgata gatattagca cctgagaaac tcgtaaaaac aggttttatt ttgttcaaaa
    36181 gaaaagcgta gggcagggaa tcgggataga taatatattt agtagaaagc atatccagaa
    36241 gaggtgtagg gatttccact ccaccttttt cataaacacc attcagataa cggtaaaatt
    36301 cactgtaatt tttgtcgtcc ccctgaataa ttttccatac atcatcatag cgttttagtt
    36361 tggcaccgct gtaaccatca atggtttgat gataatatgc ccattctccg gcagttctaa
    36421 tttgtcccat attatatgga tagatacgat aattgtcttt atcttccaaa aggaattcat
    36481 caaaatcctg agctacaaaa cgcgcttctc tttcttctgc cggataaagg tcttttaaat
    36541 gctttcccgt gtagatatac aggtctatga aagtaatcag agtaatcaac aaaaccaaag
    36601 caaccttctt cagttttttg gcgctggcaa gataacaaat acccagagaa acagttagca
    36661 gcaataaact cataattccg cttttataaa gcattccagg acgcaaactc ttcaattcct
    36721 ccaatttggc gagggcatta tatctttgat attgttctat ttcagcggca ttggtatagg
    36781 gcaaaccttt gaaaatactt tgtcccaaaa tgagaaagag aataaaaacc actccgctta
    36841 tccagaaagc ggtaaataac ttcttctgcc acttgctgtt attttccatg gaggcaattg
    36901 tatccaaacc caaacctgcc aaaataacgg ctataaattg aaccataatc aaggtcatag
    36961 aaggcacccg gaatttattg aacatcggca gatacttaaa aaagaaaccg gaaataacag
    37021 gagtaaaact gccaaagcta agcactaaaa aaatagcgga agctatccac aaaaagaggg
    37081 aaaacttttt gtgcttgcca aagagagcta taactcccaa agctaaaaca actatgccaa
    37141 agtaattata aatttgggtg aaaggcattg caccccaata attctgattt attccaccaa
    37201 agaaatcagg aatgataaag gaaattattt ctacaggtgg gaaactccat tgctgagcat
    37261 aacttttttc cagacctgtt gaacctccac gcattgtgta atgactatat tcccaggtag
    37321 aaagataggg attcatcacc gctaaggcag ttacagcaaa agcaataatc agcagtccgg
    37381 tccagttgcc gaacttttta agctgcttag tttttattgc ctcaatcaaa ccgtaaatcc
    37441 aaaacatacc gataaacaaa taaaggtaat aagttatttg aggatgattt tcccgtaatt
    37501 gcgtaatcag aaaagttgcc agcaagccca aattcaatat atttggcttt tccttcaagc
    37561 gaaacaaagc ccaaacgacc caggggatat acattatggc acgaaatttg gtattatgtc
    37621 caatttccaa caaaccgacc caatgacagg aaaaagtgaa agcaatggcg gaaaaaaagg
    37681 caatccaggg gtccattttc agttgcctta aaagtaaaaa aatacctact cctccaatga
    37741 atagcagaaa aatacgccag ttgataattt tatccgtcag cttggttata ttttccagaa
    37801 agggataacg attgggaaaa tgaatcatat atgagggcat tccggaaaac atataaggag
    37861 tccataaagc attatcttta tgcgttgcat tataatcaat tatggctttt gctgcaccct
    37921 gccattgaat tatgtccgat gcctgaggtg cttttccttg ataggcaaca ggaaaatata
    37981 tcaaactgag caggagaaaa agaattatga ccaaaaccca gggaagatgt ttggcaaaag
    38041 cagagggctc tttttccctg gcagtagttt tagcactagg catagtttta gatgttttgc
    38101 tgatattttg cctgattttc tgattgacat tttttccagc cataatatcc tctatccata
    38161 atatttttta cataacaaaa taaaagcgga tatcggtcaa gaaaaatccc gctacaacct
    38221 tttcttaaga ggtaaaaaca atgatttttg cgtttactaa aagaatctat ggctacgaat
    38281 gcgatattta tggtcatcta aataatgcca actacctgca acttttagaa ggagcacgta
    38341 gcgaagcact ttatgaaatg aatttagctc agtccgatct ccagaaaaaa ggagtccaac
    38401 tctttgtcca ttatgtggaa atgagcttta ttaaatccat tgaccacgat gaactgatca
    38461 ctattaaaag ttgctttacc gaaatgaaca gaataaaagg aaaatggcat caggaaattt
    38521 ataacagtaa aggagaaaaa tgctttaacg cagacataac aatcgttttt gcttcagaag
    38581 gtaaagcaaa acgactgccg gttgaggtct atgaactctt tatgcactac ctggaaaaat
    38641 agattaacca gctaatatat aatgtgaaaa agtagaaagg taaaacacgc ggtagacatt
    38701 gaaaacatag ccataaaata ggttgagaag gtttagaggg tttagaaggt tgagaaggtt
    38761 tagagggttt agaaggttga gaaggtttag agggtttaga gggtttagaa ggtttttctg
    38821 gtttagggaa ttgtgaaagt tgaaaacccg gagggcgaca caatgatagc gatggtggcg
    38881 taagcccctc ggaaaatgaa agccctgact tcttttcctt tttctctttc ccactaaaaa
    38941 aaagcccgga gggtgataca atcatagcga tggaggcgta agcccctcgg aaaaaggatt
    39001 taataaccct atgcttttgc tctctgccct ctgccaaaaa gggggttgtg agacaactcc
    39061 gtcgttagta tctgtcgttc ctacggaatt ttttctacca catataagtt aggattatgg
    39121 aaatagtatg cattacttta gcactgctca ctcattactg atctcatccg taatgggtga
    39181 gcaatgggta agcaatgcaa ttaaggaaga actgattttt ttgaagattt tgcttgacaa
    39241 atatgagctg agaattttca aagattttat gtaaaaaaac cgatactgaa aatcggttga
    39301 aagggaatag taaccgttca agacggaaat agatagctac attgatagct attttttttg
    39361 aagtaaattg ggtatgggga tgagataata ttaggcaaag tttgatggat tatgagagag
    39421 gagtaaggaa atagagtgtt tgactggatg gttgatgttc tcatcaacct aaagtgaatg
    39481 attggaaaac taaatcatag ttagttataa aagaataaag aggataagat agaacgacga
    39541 ggatttcgtt catccggaaa agaatgaggt aaaaaagatg gaattttaca gagcacaggt
    39601 agaagagata gccaggaaga tttgtcagga aatgcatctt gcagtgtatg acattgaaga
    39661 gaagatgtct ggcaaaggga gaattataac tgtctatctg actaaaattg ggggagttac
    39721 attggatgaa tgtgctgcct tcagtaatgc tttgggcaag gaactggaga tttttgacct
    39781 cattccggaa cgctattttt tggaggtttc ttctccgggg ctggaaaggc ctttaaagct
    39841 aaaaagtcat tatgtgagtg ctattaacga aaaagtagcg gttcaatttc aggcaggaga
    39901 agaaaggaaa tccattatgg gcacattaac agaagtgaat caggatacaa tagttataga
    39961 tgatagagga tcagttacgg agattccgtt taaatccata attcgtgcca aaacagtatt
    40021 tttagctata cccaagagga gagattaaac aatgagtgca aatatgcttg atgcccttaa
    40081 taaactggcg gcaattaaac agttgaataa ggataccatt caaacaatca ttctggaagc
    40141 cgtaacatcc actctgcaaa agcgtttgga accggaagca ggtttgcaag tttacattga
    40201 tgaattagcc ggttgtgtga aagtaaagtt caaaagcttg gttgtggaaa gggaagaagg
    40261 gttgggtcaa atttctttga tagatgcacg taataattac aatcataatg ctcagctggg
    40321 tgaatatatt gaaaaaacga tgactctgtc tgaatttgag ccgaaaatag tgaagacggt
    40381 gcagaaaata attcaggata aaatccgcca actggaagag gaaaaaattc agaatgactt
    40441 caataaacag aagcatacga ttgtaaccgg aaaaattaag gcaatagatg attatggcgg
    40501 ttatattatt gatactgggc atgttgatgc tttattgcct gtagatgaac agattgaaaa
    40561 tgaattctat cgcgtgggtg ataatataaa agcgtatgta gtgaatatta gaacccagaa
    40621 agatagagta gtaataattc tttcccgcac caatccggaa tttgtgaaaa aattgtttga
    40681 agcagaaatt cctgctattt tcagcggaga aattaagata agaaaaattg tgcgcgaacc
    40741 aggaattaga actaaggtag aattggaagc agaagacccg aaagttgatc ctattgttgc
    40801 ctgtgtagga cccaaaggaa cccgcattga ttctttgcgt aaagaattgc atggggaaca
    40861 aatagatatt gtattgcaca gtgatgaccc ggaaaagatg attgaaaatg ccctcggggt
    40921 ggaaggtata aaaagagtaa ttattgaacg caatcattct gccagcgtga ttttagacga
    40981 agcagataaa ttgatggcaa taggtaaaca gggaaaaaat gtaaaacttg ccgccaaact
    41041 ggtgggaatg aagatagata tttatacaat ggcggaattt gaagaaaaaa tggcaaaaga
    41101 aaggagaacc gttagtcata taactgaact ggatggtgtt actcctaaaa ttgcagaaac
    41161 cttgaaacaa gctggctata caagcgttca ggatatctat atggcttccc tggaagagct
    41221 ttgtaacctt gaaggaatgg gacaaaaaac agctgaacgc cttaaagaag cagcgaagta
    41281 tttctaatgc ctaataaaaa tagtaaagcg ggacacattc ctatccgcac ttgtgtggtg
    41341 tgtaagaaaa aggttgacca gaaacaactg ctcaattttt ttctcaccga aagcggaatt
    41401 gtgtttgatt gtaacagaat aatccaggtg cgcaagttct atttatgtcc tgttgccgat
    41461 tgtttcaaag gacttgccaa atggcgtaaa agtcatcaga aaaggaagat aagatgaatc
    41521 cgaaaattct tactctgatg cagtttgcca ggaaagcagg caaacttgtt tcgggtttta
    41581 atgcctgtga aagagccata gataagaagg atatttttct gttgattctg gcattggaca
    41641 gttctgaacg caccagagaa aagatgaaaa aaatggctgc gaattccgtt atttcaactt
    41701 ctgttataga aaccggaagc caggcagaaa tcagttctgc tttgggtttg cctttaacag
    41761 gtgtgtttgg aataaaagac aagaattttg ccggtaaaat tatggaatat tggctctctg
    41821 atacagagag gaggagacat tgacgattag agttcatgaa ttagccaaag agctgaaaat
    41881 cagcacaatg gctttaaaaa aacacctcac cgatttagga gtgaatgtaa aaagccatat
    41941 gagtttggtt gaagatgagg ttgcagataa aatacgctta aaatataagg aacaaataga
    42001 tgcagaaaaa cgagcggaaa gagatagaaa gcgtctgata gaaatgcgtc aggcagctaa
    42061 agcgcaaaaa tcagctcagg aagcagaaac tataaaggcg gaggaaccag ttcctcctgt
    42121 tgtagaaccg gttaaagaag ttcctaaaaa agaagaaccc aaaccagtaa aagaaccgga
    42181 aaaggaaaca cccataccgg ttaaagaaaa accggtagaa gtgaaaccag aggaaaaacc
    42241 gcataaacca gctcccgaaa aacccaaagc tgttcctaaa cccaaaccgg aagttattcc
    42301 tcccaaaaca gcagctcctg ccaaaccgga accttggaaa aagcaagaac cgagtgctcc
    42361 ttctataaaa cctataccta ctcctccatc tgtggaaatt aaaaaagaag agaaaaaatt
    42421 cggtaaggca aaaatcagtc acgaagaact gggtgaaaaa agcaaacata aaaaagctat
    42481 tatctccagc accaaaaaag ccaaacaaaa actaattgag accatagaga ttgatgaagc
    42541 ggaaatttcc cgaaatataa aaaagaccct gcaaaagagc tccaaacgga aaaaatacca
    42601 tcgggaagct caatatgtag aagatcgcag taatgaaatc gtaattaggg aatttacctc
    42661 ggttagtgag cttgctaaaa ttatgaatgt ttccccttca gatattatct ccaaattctt
    42721 tatgatgggt caattggtaa caatgaacca acgcttggac agggattctc tggaaatgat
    42781 ctgtgatgaa ttcaaggttg attaccgttt tgaagatgaa tacggaatgg atattataga
    42841 caaggaacgc gaacaatata ataatgtaaa agaagaacct cgtcctcccg tagttactat
    42901 tatgggtcat gtagatcatg gcaagacctc tattttggat tatatccgga acacaaatgt
    42961 ggttgcaggt gaatcaggaa acataacgca acacattgga gcttatcaga ttgaaataaa
    43021 caagcataag attacttttt tagatactcc tgggcatgaa gcatttacag ctatgcgggc
    43081 aagaggagct aatgttacag atattgccgt tattgtaatt tctgctacgg aaggcgttaa
    43141 agctcaaaca agagaagcaa ttgatcacgc taaagcagct ggggtttcta ttattcttgc
    43201 catcaacaaa gttgaccttc ccgaagctaa tgtagataaa gttatagccc aattaatgga
    43261 aataggagtt tacccggaac aatacggagg agatatccct tggtgtagaa catctgttgt
    43321 aactggagaa ggtattttaa atctgattga actgattctt ttaacagcag aaatactgga
    43381 actgaaagcc aaacgcgatg ttcctgcttc cggagtagtt attgaagcgg gaatggatgc
    43441 ccgaatgggt ccttttgcca ctattttgat gcaagaagga actctgcata aaggtgatat
    43501 tgttgtttgc ggagctgtgc atggcagaat ccgcaaaatg gaaaatgagc gcggagctga
    43561 attaaaagtt ttgtatcctt ctgatgttgc aagagtttat ggtctttccg atactcctaa
    43621 agcaggagac atactcaatc aggtagatag cgaaaaaaca gcccgaaaca ttagtactga
    43681 gcgtcaacaa atacgattgg aacgggaaaa ataccagaac aagagttctc tccagaatat
    43741 ttttgcccgc attaaagaag aacaaataaa tacgctgaat attattctta aaaccgatac
    43801 agatggctct gcggaagcat tggcagatag ttttcaaaag ctttccaacg aggaagttag
    43861 cgttaatatt attcataaaa gcgtaggtgg aatcagtgaa gcagatgttt ctttagctgc
    43921 tgcttcggat gccatcataa ttggattcca tgtgcgtcct tcacatcaag cgcgtaaact
    43981 ggctgaagag gaaggagtcc aaattaaact ttatcaggtt atttatgatg ttattgatga
    44041 ccttcagaat gcattggaag gaatgctaaa accgaaatat gaagaacagg ttattggtag
    44101 cgcaatagtg aaacaggtgt tcaaaattaa aagagtggga accatagccg gatgccaagt
    44161 agataaaggt gtgattcagg caaattgtaa agtccgtctt tatcggaatg atgttttggt
    44221 aacggaagat acaatcagct ccttaaaaca ttatgctgat gatgttaaag aagtccgcgc
    44281 cggttcagaa tgtggattaa ccttagccaa tttttccgat attaaagaag acgatgtatt
    44341 ggaagcatat atcgttaatg aggttaataa aaaactgtaa tgaaaaacta tcgtattccc
    44401 cgcttacagg cagaattgaa gaaactcttc aatattgctc tttctcaaaa attgaacgac
    44461 cccaaactag cttgggtgca gataaccgat gtagttatct ccaaggattt acactatgcc
    44521 aagctatatt tttcgcatta taataacccc gccagtcatg accaaatcag agaactcttg
    44581 attaagtcat ccggcttttt aaaaaaacaa atagcaggag cacaaattat gagaacaatc
    44641 cccgaattat ctttctacta cgatgacaca gaagatcgtg ccgcgaaagt agatacgctg
    44701 ctggcatcgc tcagagatga ttttgacgat gaagacgagg aagaccagga tattgatatt
    44761 gacgatattc tggatgatga tgattatttt gattttgatg aggaggacga cgaagaggat
    44821 tacgacgatt acgaagacga agaagaggat gaggaagagg aataatttcg ctatttctca
    44881 caaacttaca ataatatgaa gaggaaaaat cgcccttagt ggcgattttt tctttggtga
    44941 aagcatgcaa aatattagag aagaattagt ggagcttatt tccaaagccg aaaatatatt
    45001 acttacaact catatcagtc ctgatggaga tggttattgt tcagctctgg ctttacaaag
    45061 gattatttcc tttttaggca aaagttcttt actggtaacc gatgatgatg acctgagccg
    45121 ttataactat ctgcaggatg gaaaaagcca ggaaaagcgt ttttccgagt taaaggaaga
    45181 agagcagaac ttttctttgg caattgtttt ggattgtaat tcttatgata ggttaggtga
    45241 aagatgcgtt ctggtagaga aagcggaaaa gataatagtt ctggatcatc atgaagcgga
    45301 aaatggttta ataaaagcag atctaagtta tattgaaccc tcttttgcct gtgtaggaga
    45361 aattatcttt tccctgctgg aaacggcaat taacaaaatg cctgtttccg atcgcttatt
    45421 tgtgtgtaat tgcctttata ctacaatttt gaacgatacc aacaactttg ttaataccaa
    45481 taccgatacg gatgttttta tctttgcctc aaaattggtg aacttgggag ttaaacccaa
    45541 tgaacaatat cggtatttct ttctgcagca ttcggcagaa gaactgcgtt atataggtga
    45601 aaccctttct acaattgaat tacattacca gcgtaagata ttatttatgt attcaacctt
    45661 ggcaatgagc aaaaaaaata atattgatcc tgaaagcata atgaatgcaa cgcgttgggt
    45721 gcaaggcata aaaggaattg aggtaataac ttacttaagg gaagaaaaag aaagggtata
    45781 caaaatcagt ttgcgttctg agacaataga tgtgaataga attgctgtgc gttatggtgg
    45841 aggaggtcac aaacaggcag ccggatgtta tttttatgga actctggcag aagccaaaga
    45901 taaattgtta aaggatgtta ttaatgccct tgaatgccat aactgataaa gcatttctgg
    45961 ctatagataa gccgtctgga ataacttcct ttgcagttat caggcgtttg cgtaaaatta
    46021 ccggaagcaa aaaaatcggg catacaggaa cacttgatcc ttttgctacg ggtttgctaa
    46081 tctgttgttt gggctcttat acccgtttag cgggtttatt ggaggaaaaa gataagacct
    46141 atcttgccag gatgaaattg ggcataaaaa ctgcaaccgg cgatccggaa ggcgatatta
    46201 tacagacagc tcccattcct gaatttgtta gtaaacttga tgctttggca aaaaaagcaa
    46261 gacaattgac cgaacttcct gtgccggctt tttcagctgt aaaagttaac gggaaaagag
    46321 cttatgaact tgcccgcaaa ggtgaaaagt tcaatatgcc taagcgtcct gttcaaatta
    46381 aagaatttga atttttacct tgggaaccga ataaatctga tgaactaagc tatcgttgca
    46441 aagttagcaa aggaacttat atccgggctt taagtgaatg gctggcagaa caattgaaca
    46501 caattgcctg cacaactttt ctacggagag aaagcattgg caatataact ttgcaggatg
    46561 cggtttcttt agatgattta actaaggata actggtttcg ctatcagctt agctataaaa
    46621 aggtttttcc tgattatacc tgcctgaagc tgaatgcaga tgactttgcc aaagtgatga
    46681 atggtatgga tatagctcat tctaacgaaa ataacgaaaa atacattttg cttaaagaag
    46741 aaaatatctg tgctattggg caaagaatga ataactatat tcacccgctt agggtgctaa
    46801 aatgaataag aggaagaacg aagtcctcgt tgtttattgt tttccgaatt ataaggtttt
    46861 ggataataac tacaatagcg aaataatgaa ttttgtgtgg tggacgagga caaggtatga
    46921 aaatgagtaa aaaatcggtc ttaactattg gcacttttga cggcttgcat ttgggtcatc
    46981 agaaagtgct caaaaaagtt gtagaaatag caatggcaga aggtctgcag agtgtggtta
    47041 tcagttataa agatcatcct gcttttatat taaaaacgca tcctatgccc aaaatgcttt
    47101 gtcctgcaga aatcaaacta caggaaatga aaaaaatggg tatagagaga attgaactat
    47161 tggattttac tttagagctg gcaaaaaccc ctcccttaaa atttttgaag gactatataa
    47221 ttccccagtt tcagccagaa gttatagtta tgggttacga ttctcatttt ggacacttgc
    47281 gtcaaggtaa ctatgatttt ctttgtaggc attctgaaga actgggttac cgtgtggaat
    47341 atgtagagcc cttgcttttt gaaggtaagc caatcagcag cagcagtatc agaaatttac
    47401 tcctcaacgg taaaattgaa gcagccaatt atttattggg aagaccctat cgtttgatag
    47461 gcaaagtagg acacagcaat tccaaaggga gaaaacttgg ttttcctaca gcaaatttga
    47521 atttgctttg tccgcatcaa cttattccta aaagcggaat ttacctttct aaagtgattt
    47581 ggcaggaaaa tgtatttttc gggttgacaa atatcggttg cagtccaaca gtgaaaaata
    47641 cgggagttat tgaaattgaa acccatattc ttgattttca ggataacctc tatggtcaaa
    47701 ttatggagct ggatctactc cgttatctta gagaggaaaa gatgtttaag aatgtagatg
    47761 agcttaaaac agccatccaa aatgacattg ccttagcaag agtaatgctg aaggaatttt
    47821 cggggtgaac tatcttactt ttggactgct caattccaat tcggcagata tgaaaaatga
    47881 ggaaagttaa taaacctacc gtttatttgg caaagaggaa tttgttattt cctttagcag
    47941 ttttgttttt cctgctgcta ccggtgtttt tctctgcagt ttcgcctttg caagttgttc
    48001 cgggaagtgg cattgaagat atagaagata gcaagttata tcattttcgt tatcataatt
    48061 tggttgacga ttatcatctt tcaggaccca aaatctgggc agtgcgtttt aatttcaaag
    48121 atgcttatcc attagccgat tctgcctctt ttacgctgaa tagttttaaa atttattctc
    48181 cctatcccaa tctggaaatg cgggtatctt tatggaatga acagtatggg ggaaacaacg
    48241 aattaaacta ttttccgggg gttattattc cgggtggtaa ctggcaaaat attactcttt
    48301 ctacaagtcc cactactatt acctttcctg aaattcaaaa tcccttgcgg attatttggc
    48361 tggtgatgga ttttactaca cctttagccg ataaatatat gagcgcttcg gaaggaagcg
    48421 gaaagaacag tttttattac aataacacag ttcagggcaa tgaattctgg caaagtttgt
    48481 ttactgccgg ttttaaatgt gaattacgag taagcgcaat tggagatttt aatttattac
    48541 ataccgattt ggaactgctt aatttttctc ttccggagaa tattttgcct ggttccaccg
    48601 ttcatccttc cgttacggtt tacaatcaca gtcaatatcc tgtaagtgac actttggaag
    48661 ttaatttttc cgatcctttg ctggaattaa atcagaccct ccaaattcct ttattaaaca
    48721 taaatccccg ggattctctt tttacggtaa gttccgaagg tatttctttc agccgggagc
    48781 caacccagat aaaggtaact cttgctttca aaaatcatcc taatatcatt ttttctgccc
    48841 gttattataa tgtttttggg gagacaggaa gttctcattt agtagaatat ttccggcgtt
    48901 atacttttgg cttttccgat attcctcaag atgcatccga agttcatcat ctcctctatt
    48961 ttcctaatct ggcagataat ctttccaatc tattcgcagt gcagaggttc aattattatc
    49021 agttcaattc attgcctaaa acagtggtag atggctataa aaaatttcat ctgcagattg
    49081 atgccaattc tgaagaactt gtttccgcta tttcagccgc acaggtaaaa aggggcttta
    49141 tttcacgcag tagcagtaat atcagtatac ccgatccgga aacacctgaa aatctattgt
    49201 tgaatataaa actatataat gatagaacta ctttatatga atggacagca agttctgctc
    49261 tcaatcccaa atttttcgcc ggtatttttg aagaaaatga gtttgcaggc ggagaactct
    49321 attctctgaa aaggtggctt gtatttgagg gttctttcag cggaactctt aataaacggg
    49381 atagtgtgga aattgaatta attttgaata ctaccgacct taatccctca aaaacctaca
    49441 gggtttatta ctggttgcag aatcctttca acagcggagg tcagatttat tatgctcaat
    49501 atgtttcctt ggggcaatcc tggatagttt ccaattctga tgaatatata tctcaacctg
    49561 ttatttccgt ttatcccaat cctctggtaa aaggaaatgc tttaaaaata tccgcttctg
    49621 tttttcctgc ggtttttacg gtttataaca ttaaggggca gaaggtcttt agcactggga
    49681 aggtctttag tgaaatatct attcctgccg gtatttttcc tgcttccggt atctattttc
    49741 tgcgttgtga aatgcaggat agagacaaaa cacataaaca aatccaaaaa ataagtataa
    49801 taaagtgatg tttaattatg gcagaacata tttccatagc tgaaatgcaa caaaaagtaa
    49861 atcaggaaat cagcggtttg tatttggtag ccgaaaaaga actccgcgag gggaaaaatg
    49921 acttttatct gcgtttaaaa ctgcaggata aaacaggaac catctctgcc aatgtatgga
    49981 aggatgcagt taagcattca gaccagtttg atactggcga tattatcagc attcaaggca
    50041 cggttgtaaa ttataaaggg cagattcagc tttctctatc tcagcttcgt tttgccgata
    50101 aatccgaata taatatagag gattatctaa cgcgcagtaa aattgaaccg gaagttcttt
    50161 gcgaccgttt ttttgagttt gtggataaag tgcatcagcc ctatctgaat aaattgcttc
    50221 atcttatctt tgacgataag gagttcttta ctcaattctt aaaagcaccc gcagctaaaa
    50281 actggcatca taattacatt aacgggctta ttgagcatac cgtttccgtt gctgccttat
    50341 gtgaatttgc ctccactctt tatcctgtaa attacgattt gctgataacc ggagctttat
    50401 tgcacgatgt agccaaagtt caggaatatg accaccgcag aaatattgat tttacggatg
    50461 aaggacgtct tatcgggcat ttagctcttt cggatcaaat ggtttctgaa aatgctgcca
    50521 aaattttagg ttttcccgaa gaattattac tccatttacg ccatttaatt ctgtctcatc
    50581 atggtgaata cgaaaaaggg tccgtgcgtt tgcctcaaac cctggaagcc attgttttac
    50641 accattgtga taatctggat gctcaggcaa caggtgtggc acaattgatt gctgcaattc
    50701 ctcaaaatgc tgtctggagc gaatatgaca aactgaataa ccgctattat aaaatttacc
    50761 gacctgttta gaacttatgt tatttaagag cgaaagttct ttgttcttct cgcaatagcg
    50821 ggaatccgga taaattttaa caaaacagct gaatattcaa tagggaaatt atgttatgtt
    50881 tcaaaccgtg attattgcca gtggcagtaa aggaaattgc gttttaattc aaactgaaaa
    50941 taccgctctc cttttggatg ccggaataag tatgcacaga attatggagt gtttggaatc
    51001 atatcacata gaatcggcaa aaataaacgg cattctcgta agccatgaac attccgatca
    51061 tatcaaaagt gtaggagcta tttcccgtaa actgaaaatt cccgtttata ttaatagacc
    51121 tactctttct tcttgtgcag agcgtttggg cgatgtgcag gataggatag tgctctttaa
    51181 cacaggtaac agttttacca ttggagatat aattattgag ccgtttgttt ctactcatga
    51241 tgctgccgag agctgtaatt tcgtttttta tcctgtttct gagcccaagc gtaaattggg
    51301 tgtggctaca gacctgggct atccaacgca attatgtctg ctaaaattga gcggtgtaag
    51361 cactttaatc ctggaaagca atcacgatga aaaaatgctg atggaaggac cttacgactg
    51421 gaagctaaaa caacgcattc gcagtttgca aggtcatctt tctaatgtgc aagcagttgg
    51481 cttaatttct gccctaattc atcccggttt gaaaaacctt attctggcac atttaagcga
    51541 aataaataac cttccttccc tggcagaaca aacaatgcgg gattatctga atagtatccg
    51601 cagcgatata aaactgtttg tggcagaaca aaataggcct actcccccaa tagaaattta
    51661 aagtatcgca cctctttgag ttaaaatcgg cttttctcaa tccttttttc tcgtttcatt
    51721 attgtaaata tctttcaacc ccttttctct tttacggttg ccttctgccg attttgactc
    51781 aaagttgtgg gaaagttgtg gttgtcttct gccgattttg actcaaagct gtggtaaact
    51841 cgttttctga atcggctttt ctcaatcctt ttttctcgtt tcattattgt aaatatcttt
    51901 caactccttt tttcttttac ggttgccttc tgccaatttt gcctcaaagc tgtgggaaag
    51961 ttgtggttgt cttctgccga ttttgcctca aacccgcgtt aaaattccgt aggaatgaca
    52021 gagaaataac gacgggtttt aacccggcga aaatatatct ctgtgaaaaa ggaagagttc
    52081 cgtaggaacg acagatacta acgacgggtt ttgaacccgg cgaaaag
//
LOCUS       DAOOZV010000005        50507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_789_length_50507_cov_64.913403, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000005 DAOOZV010000000
VERSION     DAOOZV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 50507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50507
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_789_length_50507_cov_64.913403"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..4356
                     /locus_tag="PLW08_01120"
     CDS             73..4356
                     /locus_tag="PLW08_01120"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPL51240.1"
                     /translation="MAENITQQLDAYITNKIALSKAMGYLSDSAKSHLIEIVGKSGSG
                     KSHFVQPLMESVEDIYAKRIYFSPHPLMLNHFSELMQVITEVTEEDLYGYYREFRDNI
                     GSVNKYDFFFYITERLNAMELFQPFLLVIDDCDILDNYTRDYLQYLVQYLPDTGIQIV
                     VFTQTRLFPFAELDNIPTLGVEECQRLLQLTFPETDFTGSSESEIFQKITSGNLMIIE
                     KVLKEMKSQKPKGKFNLGPYLEKTFDAEQIYYETLETLSSSQKEILTAMYVLDGFDAS
                     ALAKALGNPQNIKTDLVTLYNLNLISKPEKGLSIQKKKAFSEWLNNNLKEFKGTLLNQ
                     LMEYLQKEKKHPQILVSLQMLNKKYNSALFGKVISELSQINDSASVLRLEEYVLAHTK
                     SPADKLASTQRIAEAYSVLNQKDKAIDHFRQALHISTENNIPAEQIVYKLASDLYAVN
                     SSAFALEIIKQYSPATIDPYWKVKILLLKADIQTESEAFNEAFNTLDIASHSSNSITN
                     QQQRHSVQAEAKKIRGKIHYYINEWEQAEEVFNEAETLYSLAKDHSGLAAIYNNLGVL
                     YMCQGFWEKSETYFLKSLALEKQYFNLNGISVCYNNLGGLMDDKGDPAKSLYYLEEAL
                     KIQKLLAEPYNITNIYNNIGVTLMDHGDYDRAEDALKKSLDMANEFGFQRNTIATLNN
                     LGGLYFKKGDWAESISYYEKAIKLSQESGFNEGLLRSFNNLGEVYEKQDELNLAYDLY
                     IKGLELLPAVSDDYIKAELYGNLGSVFTKLHKFKDAYRYLVESLDFFKNIAARDKIIE
                     GYLKQAYYFILTRNIESADYYLAEAQKLAIEQNLPFEIGRSLYLRALLERKNLESAKD
                     YLNEAIKHFVEAGSNYELSLANYELAGVLLDMEEWEQAMEILKNNRKIIERYGSIKLL
                     EQNDILMQKISREYSSQMEEVKFEENLLNQFYEITQNLNTITDLDVLIEQSLSSLVEI
                     ANADGGMLLLHPNPSLPDAWEYRIYHNFSPEDKYHDQFQNICYEVYQNNKLENIKQPH
                     FAPSYNNILALPLSIRKNTMGVVLLFCKSGSKYFSERIINLLSALSNQIIVIIENILS
                     ANLEKSHNVIREQLSSNNIYSNIIGKSPEMMKIFEIIEKVKDTPTTVLLEGPSGTGKE
                     LIARALHYSSNRRNKAFVAQYCGALPETLLESELFGHVKGSFTGAAYDKKGLFEIADG
                     GTFFLDEISDISQSTQSKLLRFLQEGEIKRVGATKTEKVDVRVVCATNTPLMERVKSG
                     EFRLDLYYRLNVIRIDVPPLKNRTGDIPLLAIHFLDKYNKRMNKNVMGFTAEAMKVLE
                     QYDWPGNVRQLENEIERAVTLAEPNTFIKPSDFSEEVYRHIEYSKTIAMLSGKKNLKD
                     AIEELERKLISQSLEKFQWNQTQAAKELGLSRQGLIKKMQRYNLYREED"
     gene            4372..7254
                     /locus_tag="PLW08_01125"
     CDS             4372..7254
                     /locus_tag="PLW08_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPL51241.1"
                     /translation="MESINNEVKNTENFNNLPLEQLEFVAIDIETTGLNSEWDEIIQI
                     AGMRFKGEEVVNQFVSLVKPKGKVPKFIEHLTHISPQELKTAPPIKEVLKDFCNFIGD
                     SILVGHNIGFDFNFLNQFIVDNGGFPISNMHWDTAEIGRIYLPFTTDHKLSTLVSFFG
                     IELMNAHQADADALATGKLFNALTNYILNHYGYMLNARLMDLAMQANLKSGLYEYFKW
                     IVRQQRSSALSGKKTVPVVNELNNVIEHIAAGSIKSIPEVFAEDGIFASKFPNFEFRS
                     GQMTMAEEVADAFSKQDFLVVEAGTGVGKSFAYLVPAIDFSNRNKSKVIVSTNTKNLQ
                     EQLFFKDLPQLKKILPLPFQAVLVKGRENYICERRWQELLQEQTRGITSYDAYGLLNL
                     LVWKEHTNTGDVSENSSFDKNRFGVLWHKICSDRYLCGGRKCPLFHSCYVMKLRKNIE
                     TASIVVANHSLLLADAQMDNSTLGEYQYLVIDEAHNLMSAASQNLGFTLSYVDLNSLF
                     NQLVYTSRKYSGGFLSQIMQVLQKSMLTTDKKTHIETLCTKLTGEIDSQRQVITKLFS
                     LAEDHCVEAKSYNKLRIKDINAFEDIYTLLKELSLAFKDILKDLTALDNVFSTLNSNQ
                     VKDLDVLKDNLKGYLSRYMETEEIILQLLNPDLDNYALWIENDPISERNVPISTFCYA
                     PVEVGEHLNRLLYKKIPCIIFTSATLSIRGSFKFFLNQSGLSLVTEKNVKQSIVESPF
                     DYDKQSLLLIGSFLPEHKDKFFQSQALGCLEQILTTTNVGTMALFTSYKDLDAVYNHL
                     SDTLFHLNRPFFAQGKGVSRNSLLDEFKKSKNAVLLGTSSFWEGVDIQGDSLSLLILY
                     KIPFQVPSEPLVEALIDKLERENKDSFMHLMLPNALLRLRQGFGRLIRSKTDRGVVLI
                     MDSRVSKKKYGIYFKQILPGRCIELKDEQHLISEIGKFFNPLPANI"
     gene            7272..8192
                     /gene="murB"
                     /locus_tag="PLW08_01130"
     CDS             7272..8192
                     /gene="murB"
                     /locus_tag="PLW08_01130"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPL51242.1"
                     /translation="MLRKEYPQLIDEGIVHIEVPLKEHCSFNIGGPAEVFCTPYTQKQ
                     LVTLLKFCLEHNIPYFILGKGSNLLISDKGVKGIVISTERFDKITLGKHYLSAFCGVT
                     LKDLSDFACENGLSGLEFASGIPGSVGGAVFMNAGAYGNEIKDVLYSSRAILPTQEKL
                     NSPNPVLYLKVSEHNFSYRHSIFQDLGLIHLSSLFILKKDKPENIRQRMQELQQKRQD
                     KQPMDFPSAGSVFKRPEGFFTGKLIEECGLKGFRIGDAAISEKHCGFIVNLGSATAKD
                     VFQLIKHIQKVVYERYGVHLQTEIRILGEI"
     gene            8189..8968
                     /locus_tag="PLW08_01135"
     CDS             8189..8968
                     /locus_tag="PLW08_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51243.1"
                     /translation="MKSRQIILPLLFFVLLVLLWQFISTKGVIAFWVVPSPQQVIQVF
                     LDYPGLIWHHLKPTLIEAISGLLISIVLGSVTAIAMNLSRLIKQIIYPYLIISQTVPI
                     IAVAPLLIIWFGYGISAKIFAVVLMCFFPIALGLYDGFRSVSIDQIRLLTSMGATPYK
                     IFRLLKIPSALPNFFTGLKLSATYSVMGAVIGEWLGGTSGLGIYMTRATKSYQTAHIF
                     AVIIVIVAVSLALFGIVALLDRILLKWKYHPIDEYLEPEKK"
     gene            complement(9091..10359)
                     /gene="mtaB"
                     /locus_tag="PLW08_01140"
     CDS             complement(9091..10359)
                     /gene="mtaB"
                     /locus_tag="PLW08_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPL51244.1"
                     /translation="MIRIAIATLGCKANQAESSIILDQFNNFKLVPWQEEADIYIINT
                     CTVTNRTDYKSRYLIRQALSQKAQNPFAKIVVTGCFAQRYPEEIAKMGNIDWIIDNQQ
                     KLNIADILAGGNYEFMDIMQAKEFVYKPTAKMYNRTRAFQIIQDGCDFNCAYCAVPYG
                     RGKSRSATLEQVIQQAKLFVENGYKEIVLSGINLGLYKDGNNDLTDVLLRLNELQGLE
                     LIRLSSVEPQLFNDKLINTLPLIPKLCPHYHIPLQCGADSGLKRMRRHYTSTTINQLI
                     IKICERIPYPAIGMDVITGFPGETEEEHNQTCEFLRSLPLAYLHIFTFSKRKGTPAFD
                     FPNQISKTIKNRRANELSQISRELTSAYTKSLMENNIPLRGIVEKNTNGYCEFLSDHY
                     VRVRFSGNFQPGDFVQVPSQEAQIKMKNEE"
     gene            complement(10356..10958)
                     /locus_tag="PLW08_01145"
     CDS             complement(10356..10958)
                     /locus_tag="PLW08_01145"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPL51245.1"
                     /translation="MERPASATHKIAVFCSGLSRGSNLCALHNYFTQNKLPIEVALVI
                     FTRKDAPAVQLAEEKGLNYHIISTRNMQLFEQQAINLCQQHNIELIALAGFLKQLSEN
                     FIEEVQVPILNIHPALLPQYGGKGMYGMAVHKAVFASCDKFSGVTIHLVNSQYDKGKI
                     VAQQKVDISSCKSPEEIAEKVLEIEHKLYAPAICQFLLKS"
     gene            complement(10925..12706)
                     /gene="aspS"
                     /locus_tag="PLW08_01150"
     CDS             complement(10925..12706)
                     /gene="aspS"
                     /locus_tag="PLW08_01150"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPL51246.1"
                     /translation="MLDNLGNLTRTHYCGNLNTEHIGQEVTVMGWVNKRRDLGGLIFI
                     DLRDVQGILQVVIRPEKPEIFAKAEKVRNEYVIAVRGKICARTEPNINPNLPTGKIEL
                     EAEEFYILNDAQPLPVQFSEVAMAEEDLRLTYRYLDLRRPKLQKIIITRHRIMQIIRE
                     FLNNEGFYEIETPILMKSTPEGARDYLVPSRMQPGKFYALPQSPQMFKQLLMIAGLDR
                     YYQIARCFRDEDLRADRQPEFTQLDIELSFVSQAQIFDLIERLFAKLFQEVLGYNLSI
                     PFPVIPYAEAMENYGCDKPDIRFEMKLFDISNIVKNSGFNVFSSALKNGGVVKAIAIP
                     KAADFSRKQQDELVELAKHLGGQGIAFAKVTENGLEGGISKFLSPEEAEAIIQATQAQ
                     KNDLLAFAADNYEMVSKVLAGIRNFLAPQLNLIPENSFAFCWITDFPLFTKNLETGKW
                     EPAHHMFTLPKEEHIPYLDIPEKIGDIIGQLYDLVCNGVELSSGSIRCHRYDIQKKIF
                     DILGFSEEELQKRFGFFLEALKYGTPPHGGIAPGLDRLVMIMTGAESIRDVIAFPKTL
                     KATDLMSQAPSEVDEQQWKDLHLQPIK"
     gene            complement(12730..13788)
                     /locus_tag="PLW08_01155"
     CDS             complement(12730..13788)
                     /locus_tag="PLW08_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPL51247.1"
                     /translation="MKKGNIIVLIMVIVLLFFCGSCSKNKPVLYIFNWSDYIDPDLIK
                     EFEQQNKCTIKYSTYDSNENMLTKIMSSREAFDLVFPSGDHVTIMCNAGLLEPLDLTK
                     IPNYRNLDNNLLLKATSFDPGNKYSLPYFWGLTGIIYNQRYVPESVVKSQSWNILADN
                     FFTGKNKVTMLDDAREVVGAALIYNGYDLNDTSAEALAAAEKTLAEWDKNITQFDSDS
                     YKNEVPDGTTWLAQAYNGDALQVMENNPDIKFFLPVEGTSLWMDNIVMLKSSKNKELA
                     YKFIDFLLNAEIGKRNTEYCRYATPNKAANNLLPDTIKNNALIYPSEEYLQKCYMINA
                     LGENVKKIDKLFEAIKLN"
     assembly_gap    13864..13963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13964..15183)
                     /locus_tag="PLW08_01160"
     CDS             complement(<13964..15183)
                     /locus_tag="PLW08_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL51248.1"
                     /translation="MKFNRNIIGWMLYDFANSAFTTIIVTVVYSVYFINNVVGGPAGY
                     GEMLWGRAIGISMTLVAISAPIFGAVADYSRAKKRMLFINCYITVIFTALLYFVKPGD
                     VFIGMLFFIIANFGFNSANVFYDAFLPEICQPEDIGKVSGFGWSLGYVGGLVSLLVAL
                     VLVKINVRLVFPAVALHLFLFSLFTFFWLKEFHRPSQRSNYYRIAWQRVTYSLKNIAK
                     LPQLLKYIISYFIYNDGITTVIVFASIYGAERFGMTTQQMITYFILAQFTSILGAAFF
                     GWLTDKWNVKNSLSISLIIWIGVVIWAFFCSSASEYYFVGLVAGLAIGSSQANSRTML
                     SLLTPRDRQAEFFGFYTLTGRLSSIIGPILYGWIAHQTGDIRYSVLSLIFFFVIGWIL
                     LQSVQLQEGIEQAKV"
     gene            complement(15180..16256)
                     /gene="recF"
                     /locus_tag="PLW08_01165"
     CDS             complement(15180..16256)
                     /gene="recF"
                     /locus_tag="PLW08_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPL51249.1"
                     /translation="MNLAKIELENFRSYRQNEFDFNPQGCLIIGPNGCGKTNLLEAIA
                     YCSIGKSIRFHHDEELLNFEGQFFRVQSLFISDQQTPKKISLSYADQHKLLKIDELPI
                     RQLSSLFEVVKVIYCAPEDHLLISGSPRFRRQYFDLAISQLYPPYINVLRHFLHIVQQ
                     RNAMLKRNYSRAEITSWNLSFASSLAEVWNYRNRYLKQVNTAFQETFKDIFPASTAIS
                     LAYIPSLKLPLESSPEEIIKHLATIEEREKLLQRSLVGAHLDDYEFKLKGRKMLTYAS
                     QGQKRIAVIILKLIQARLIETVTGIKPIMLFDDIFAELDTFHSQQIRNCINNRYQVFI
                     ASPKEDLCNIWQGYQIQKLEGIDK"
     gene            complement(16261..17430)
                     /gene="dnaN"
                     /locus_tag="PLW08_01170"
     CDS             complement(16261..17430)
                     /gene="dnaN"
                     /locus_tag="PLW08_01170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPL51250.1"
                     /translation="MRLAIEKKDLLARIQHLVSIVPTKNTSPILNNYLIEVSEENNKV
                     RISTTDLELMVVVEFPAAVSEGGTVAVSAHHFNEIINYMPDGVINLWRNDDLLMIQCG
                     KVDFNLLIADHTLFPVIPEPKLNNPITIDAELFSRMISKTYFAVSTDVNRPVLNGVCW
                     KIYPDHHIMAATDGRKVAEIKILNSSLLSSLEPNDNPEMNIFTEQNQYYVEKVIPVKT
                     LLFLQKIFNDEVKELKVGLEHNRILFLYGEYFISSQVLEHKFPDYQKAFPAELPNKFV
                     IDRQALITAIKRVALVAPDDNLRIHFDLDSDRFEVNTSNRDTGEAKENMDDFTFAGSS
                     TGISFNYKYMLSILEAIDTEKVVIKLGTSKDAMMIYNETPLPDQEITFLLMPLRS"
     gene            17677..21756
                     /locus_tag="PLW08_01175"
     CDS             17677..21756
                     /locus_tag="PLW08_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPL51251.1"
                     /translation="MEPLKSKALAENLAITKVAEIVLDDEAKWLLSLTEGNFGINQRC
                     RNFLEELYHPYANPEAVASLIRQSILGDLWFYVQLPDKEKTLTVIGDLYLLAAKKCTK
                     PMQEKRIIIEYLDFISALCDKEGIPESVYAKLLNELDNWQKNSRELFIPLNGACRKCL
                     SNLVQVYKDNNICFRMLKDLLKEGLEFWQKTTDIEGWFAKMGAESNLDVKQLSSTLGK
                     KYYQKWLKQLEEIQDFASLKDIPSFSDIAVLQREAICEFSTLTLRIQYIFYLLGLPGM
                     EDLRNHLLWDLNRQLADLEKDLKEDEVPLMLDSIFETLYEFKETHLSIVLDCILTIGK
                     TVLSHKNKKHCQHLLPKIMELEFTPPGKIKIDKDWQMEVDKNHIKHLRMLLELIAIDP
                     VMCKDLLAYLIISLSQKGVFISDTDLFQKDVSAFLNSNFKPLVVQTKHLLKMFPVFFN
                     EIGAEGEIRDISTSLDELGHRKDLLMHFLRKQLHTESNNTHLLLIQKILQYWTNLDYR
                     ELQDMIPEDVLIYVMHPDEMTKKQSAVVNKFLQKHNLTPEELLSLSWQRVSKLFENEP
                     EDFSLKRLKLLCYSHFLLKDKYNLDPYDIVKFLTRYSWFDAHEHNRLANSFMRKDYDS
                     SIRQLLSYIGKMNSVILDPQPTTAWENIYYKRHIAAGIPSMYGMYREPKLEAMGMIFR
                     IENVVKRLFERNVQQLNLSYITGKTLKRIIRILELYDYAMKQILVTSDAYGSALNMLR
                     SAQYLSNLSIDQYLDIFKLLKDSVVEIINEYYYRFYEGQLRQIGMRIAEKEKGKKGRG
                     TKEIEIFAEEFYRNLLSKSFLVQGLDNFITRILESLSEMKNLFKPEEIPKVMSYDPDK
                     LFFHLNKKNSRIENQVLLGSKAYFLKRMYQYEFPVPPGFVITTDLFRHRNIINTHPDI
                     SNELDNLILQNLNILEKNTGLVLGDPERPLLLSVRSGAPMSLPGAMNTFLNIGLTDEV
                     TYKLSRRPNYGWTAWDCYRRLIQSWGMAFGIDRDEFDAVMLNYKKKYNVELKTQFSDR
                     QMQEMVQDYQKVLAKHNIELEQQPFMQLYKAISNVLDSWNTDRARLYRQKLHIADEWG
                     TAVIIQKMVLGNISLESGTGVLFTYADFNEEGGICLNGDFTLCSQGEDVVAGLVHTLP
                     ISEMQRLHSKTAVSSSLENTFPAIYNALMRYVKQLIEDKNYPHQEMEFTFEGSSPDQL
                     YILQTRDQVIQKAPDYKVFGTTKGEQKKLAYGIGIGKGAVNGIIVFSKEDIEKFAGKG
                     EALILVRPDTVPDDMELLFACQGLLTSRGGVTSHAAVTATRLGLIGVVNCRDLVVSES
                     DSNCRIGSTYLKAGDKIALDATSGAIYLGHYPLVSVHCL"
     gene            21786..23036
                     /locus_tag="PLW08_01180"
     CDS             21786..23036
                     /locus_tag="PLW08_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPL51252.1"
                     /translation="MKVNLGNKKLLGINGLGRIGKLLLWNQLHLKYFDGIVVNCGRIV
                     GKTLDDLIQVIEMDSTYGSIHKFLYGVVGKKAEIKILDAEEPLLEIEGMLVKILRTAR
                     DPKDINWLNEGVRIVVDCTGNFLDPTMHTDSGKPCLRGHLDAGAIKVICSAPFKIKSG
                     NTVPDDAKTMIYGINHLEYDPLKHNIISAASCTTTGLAHMLKPLLENQKTSRILTASM
                     STIHSATNTQSILDSVPKTDASDLRKSRSVLNNIILSTTGVTKALEQVMPNIKHIGFM
                     ADSVRIPTTTVSLINLNVTFHTELDELGEPLVNRKLINEVYQKAAENSQKDLLLISHR
                     QNVSADMIGTMAAVVIEAHETHTRTGFITIPSSTLAEQGILTDKVLEMPVTHAKIFGW
                     YDNELGSYVNCLSRLTNYIEEKTL"
     gene            complement(23242..23529)
                     /locus_tag="PLW08_01185"
     CDS             complement(23242..23529)
                     /locus_tag="PLW08_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51253.1"
                     /translation="MKNEEEKSGGEAVRIGQRYFQRKGKLNFYLFLNQFPTSTQHLGW
                     VEVGWRIGCWQGTSKKYFQKGTNALIKSNNKIEGIGRGKIPLFCTIKKANN"
     gene            23652..24965
                     /gene="rsxC"
                     /locus_tag="PLW08_01190"
     CDS             23652..24965
                     /gene="rsxC"
                     /locus_tag="PLW08_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPL51254.1"
                     /translation="MRLKTFPGGVHPPDEKRYSASVPITEAPIPQKVIIHLSQHIGSP
                     SKPIVAVGDEVLTGQKIAEATGYVSLVQHSSISGKVTAINRFPNATGSSSMAIQITGD
                     GSDKWIELVDEPNVMDLPLEEMKKRIGEAGICGMGGAGFPTFVKLSPPADKPIDTVIL
                     NGVECEPYLTSDHRLMLEKGEEILIGLKLIMKILSAKKGMIGIEANKPDAIAKMQELT
                     KYEPAMEVVPLKLRYPQGAEKQLIYAATKRKVPAGGLPLEVGIVVQNVGTAFAIYEAI
                     RYQKPLIQRVITVTGSPVTKPQNLLARIGTPFSELVDFCGGTKEEVGKVICGGPMMGF
                     ALPTLEATVGKGSGGLVLMNTENARLNEEGNCLRCARCVDVCPLNLVPCMIVSAVKYD
                     NQKLAVRSGLNDCMKCGACAYVCPAQIRLVQYIDTGKIRYAEAKK"
     gene            24975..26078
                     /locus_tag="PLW08_01195"
     CDS             24975..26078
                     /locus_tag="PLW08_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPL51255.1"
                     /translation="MKDRFIVSPAPHFHDKTTTKNVMWNVVLALTPALIFAIYYWGFN
                     SLLLTLLGAVSAMVTEAIIQKFRKVPITVSDGSAFLTGMLLAYNINAGAPWWVPILGA
                     AFAIAIGKQVFGGLGNNPVNPALLGRAFLMASWPTIMTAGWAKPKSGTLSGITDPNVI
                     AVNLQNLSPKAYDLVTGATPLKVAQTLRDSSFVSSLSANAGQGFDLANRIFTNLTDLS
                     ALKNLFWGNIGGCIGEVSVFALLLGAAYLLYKNIIEWRIPLFYIGTVFVLSFFLAPLK
                     GSGFSPLLPFFHIFSGGLMLGAFFMATDYTTTPLTKSGRIIFAIGCGILTMVIRLWGG
                     YPEGVSYSILFMNVLTPLIDKLTMPKAFGRVKK"
     gene            26075..26665
                     /locus_tag="PLW08_01200"
     CDS             26075..26665
                     /locus_tag="PLW08_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPL51256.1"
                     /translation="MKIYLQLGLILLAFCVAATALLAYVNSLTKPQIDSIQQKEAEET
                     RRKLIPDSEFEEIKGDFTYYIAKDNKTKEVKGYVFTAKKNGYNGPVKTMAAIDKDFNI
                     IAIKVIQQTETPGLGTNSTQPKFTDQFKGKSVEQLIVDKDGGVPPNCIKAITGATITT
                     RAVTNSLKESITLLKKLVLEKSASTIETQVREEVHK"
     gene            26662..27252
                     /locus_tag="PLW08_01205"
     CDS             26662..27252
                     /locus_tag="PLW08_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPL51257.1"
                     /translation="MSFMKELTKGIIKENPTFVIVLGMCPTLATSTSVNNAIGMGLAA
                     TFVLICSNIFISLIKNITPDKIRIPVYVIVIAAFVSIVDMCMAAYLPALHKSLGLFIP
                     LIVVNCIILGRAEAYANKNNVINSIADGIGMGLGFTLALSVVATIREILGNGTWLNMR
                     VMPKTYDPMLITLLAPGAFITLGLLIALMNMLKEKK"
     gene            27252..27854
                     /locus_tag="PLW08_01210"
     CDS             27252..27854
                     /locus_tag="PLW08_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPL51258.1"
                     /translation="MGFLGQLFTMAISAILIQNFVLSRFLGLCPYLGVSKKMDSAFGM
                     GMAVIFVMTLASAFCYLIYQYILVPFELEYLQTLAFILTIASLVQFVEMFIKKNSPAL
                     YKSLGVYLPLITTNCAVLGVAILNITPLADGTRYNFIWAVLNGFLSGVGFTFVLLAMA
                     GIRERLEMVEMPKSMRGMSSGLILAGTMALAFYGFMGMKF"
     gene            27869..28732
                     /locus_tag="PLW08_01215"
     CDS             27869..28732
                     /locus_tag="PLW08_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPL51259.1"
                     /translation="MLYLIVVPMAATGFSAILLPVAIMGSLGLIFGLILAFASKVFPV
                     TVDDRIEKIIKVLPGANCGACGQPGCSGYATAIVNEGKDLNLCAPGGPAVVEKIAQIM
                     GKEATAKDKKIAVIHCSSGGKNNTKWKYAYQGIESCLSAVNIADGPNLCSYGCVGFND
                     CVAACKFDAISIDNEGMRIIDREKCTGCGACVTACPRKLIMLVPASMNVFISCSSKDK
                     NPLPKQNCGADKPCIGCGLCAKKCPAEAITVENNIARIDYSKCTDCGTCATVCPTKAI
                     IDLKAGSRPEA"
     gene            29132..30883
                     /locus_tag="PLW08_01220"
     CDS             29132..30883
                     /locus_tag="PLW08_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51260.1"
                     /translation="MRTFDAVLPYLKKSYTRILGGLVMLILVDLVQLAVPKVMQYAID
                     HIQERTIDQQGLLWIALIIFAFAIAVMVLRFFWRVLIIGNSHSIEKSLRQDFYDHLLT
                     LSQNFFNKSKTGDLMAYATNDLNAVRMLFGMGLISAMDIVLMTVASFAFMISINGKLT
                     LLAVIPMPIISLTVMYFGKKVHTRFKAVQKSFATLSGRVQETISGIRIVKAFCQEKTE
                     LKKIDDVSHDFVNQNIALAKIAGVFHPFMGFIVSISMIITIYFGGKSAIRGEISVGGF
                     IAFFQYLGMLVWPMIAIGWIVDIYQRGTASLKRLNEIFEIKPEIDDVFADERITHLEG
                     KILFQNLSFRYSDNLPLIFKDITAGIEVGKTLAIVGPTGCGKTTLIELLVRIYNPPRN
                     SIYVDDYELYTIPLNVLRRDIVLVPQDIFLFSESIADNIRLGNPDASDEEVFEAARIA
                     QVYDEIMEFEQKFETVVGERGVTLSGGQKQRVAIARALLTNPNILILDDALSAVDTKT
                     ERRILNRLIEIRKGKTTLIIAHRISSLQHSDLIIVINEGIIAEQGTHQNLLAKGGLYK
                     DLYEKQRIRARLEGEEE"
     gene            30883..33051
                     /locus_tag="PLW08_01225"
     CDS             30883..33051
                     /locus_tag="PLW08_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51261.1"
                     /translation="MHGGGGGGGSIGRGTYAEDELKTKIYNKRLFGNMLRYLKPYLKW
                     VILSFLFLMLISGAEVIIPLIQRSAIDDHIVSDKSIAVFAGENDYQNFIKRYQKLGFK
                     EYKYGENHFIIINAKDRNKINRTDLEYLQEKGILKAETVFLLSDKDENINILKKYLQE
                     NLHPDTPNLSGWYKIGSKTIAVPKKELNKLPKEERLKVRNETVQKLLILALIFLAIIT
                     LRFIASYFQTVFTAYFSQNAMADLRHDVFAHLQKMPTKFFDTNPVGRLVTRVTNDIAA
                     IDEMLASGVITLIQDVILIITIVILMLALNWRLALVSFSILPLVIWVIVIFRKKTRVV
                     YREVRKHLAVLNATLAEHIEGQKIIRLFNQYFHKRQEFATINQKYYLASIKQIRLFAF
                     FRPIIHVSSQIAVALIIWYGGGKILQNVITIGLLMAFTQYISKLFEPINDFSDKFNFL
                     QSALASAERIFNLISLTPDDYRVEMHKNIKMKGEIEFQNVWLAYNDEEWVLKDISFKI
                     APGEKIALVGHTGSGKTSIVNLILGMYPFQKGRILIDGKDIHNYSLADLRRNVGIVQQ
                     DVFLFSGNIKDNIALNNNDLTDEEIIRVAKYVNADKFISQLPRGYYEPVMERGATLST
                     GQRQLIAFARVLAYNPSIFILDEATSNIDTETELLIQDALNKVIKGRTSIIIAHRLST
                     IQNVDRILVLHKGEIVEEGSHFELLDKQGLYYDLYRLQYT"
     gene            33277..36084
                     /locus_tag="PLW08_01230"
     CDS             33277..36084
                     /locus_tag="PLW08_01230"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51262.1"
                     /translation="MKKYWLIILALTLVTAIFADGNRYEFIAYGEDFESGAPGWTHYD
                     ATVSPNNWHIYNFGGTQGNVWWMGDPALAQGSNIGGYYNHQYLVLDTPARTLTSANAT
                     LTFKMRHNVENPAGATAPWNGWDACNVRISTNGGATWTPISGNPAYDFTSAYSFGFEH
                     GEGQGIPGWGGVREWTNCTFDLSAYVGQSVKIRFAFASDPAYCTSDAPAMYGMMVDDI
                     SFGGYSNNGVDDGQMTYSSLVPVGEDLWHIATDATAPSPSHCMVCQNASGSYNPNMLN
                     YLVSPPVTLPNSGDMWVDFMLKGSYNDPNAFPEVDYFGWEISVDGGNVWYAMSNPYGT
                     PGGDNYVYTGLQNTWQSMTEGFGLSFFITEYHGETAIFRWYFKSDGDTPDGVGMFIDD
                     FRVYNDVFIAAPENLTAAVSGNSVTLTWNEPGSGGGGGEEGWIYYCGEPAGNSIGTNG
                     AADFSVAAKWDAIGETNSIYPYVGMNITKIKFVPAEPTTLCSYAVRIWTGAAGVLVYD
                     QAVPTITEDVWNEVILTTPYTIPAATTIMVGYRCNTQGGYPAGCDNGPQVEGYGNMIY
                     MNNNWHTLTAESASLTYNWTIQVYVADATGKEYVLGELPMNNSFAEGKFGVHYTEPVR
                     GRDVTSYKVYRDASYVATVDGNVLTYTDTQVPGGPHSYYVTAMYGTVESGASNTVSVF
                     ILPAEHYEINHDDGTAEQGFSVGATKQMAVKFECNDEYDLKYVKVYVHTLGSAGLVIR
                     VYDANGPNGMPGAQLVTQYQYPAASVVAGWNFITLPTPVHLNNQAFYVALFETSGASL
                     IGLDTSSNGFSYQNTSGSWVPVTTGEIMVRAIIQGGNAGEDLTLPPLVLDARNYPNPF
                     NPETTISFSIPASGQTSVKIYNLKGQLVRTLVNETMNAGYQKVVWNGTDDNQKSVASG
                     IYFYRVNNAGKTITRKMLLAK"
     gene            complement(36782..37534)
                     /gene="phnC"
                     /locus_tag="PLW08_01235"
     CDS             complement(36782..37534)
                     /gene="phnC"
                     /locus_tag="PLW08_01235"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HPL51263.1"
                     /translation="MNPNSNLLQVNNLSKSYDGKKWALEDLSLKVQEGDFVILLGLSG
                     SGKSTLLRCINRLIEPTRGQILFRNEDILQLKGIELRHYRRQIGMVFQQFNLVKNLTV
                     LTNVLTGRLGYHSSLSPFSKEDYELAKQNLEKVGLQDFADKQVKFLSGGQQQRVGIAR
                     ALMQEPSLILADEPVASLDPATADSIMQYLGEINREGISIICSLHFLTLARRYGNKVL
                     ALKEGQKVFEGIPNQIDNIRFKEIYGQDAEEI"
     gene            complement(37531..38457)
                     /locus_tag="PLW08_01240"
     CDS             complement(37531..38457)
                     /locus_tag="PLW08_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPL51264.1"
                     /translation="MCKKYIWLAFVALLIFGCGTEESRLEKANKKLGSKQNPIKMYFV
                     PSLEAGKVVSSGEAIANYLEKETKLHFKVAVPTSYAAVIEALGTYQADVAWLPTYAYI
                     LAKQKYDAQVRLMTVRNGLTKYRGQFVARSDSKINSLQDIEGKIIAYTDAASTSGYIY
                     PSAILKQRGITPKDYFFAGGHPQAILAVYSGRADVGCSYWSPPDAKGKPMDAREKLLE
                     TYPDVFEKVKIVDYTDWIPNDTVTFRKDLPPELEGIIVQALYRYAQSAEGKKVLKALY
                     DIDGLEYASDKDYEIVRTTLKTMNMDPAELLK"
     gene            complement(38527..39798)
                     /locus_tag="PLW08_01245"
     CDS             complement(38527..39798)
                     /locus_tag="PLW08_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPL51265.1"
                     /translation="MAILIQNVLSRSLAAKNDIKSGEKLISVDGQAVNDFLDLEFYTS
                     DYEFELEIMSAEGISRKVKIQREEKSFLGIEPEPYKIRYCENACIFCFIDQMPPELRP
                     TLYIKDDDYLYSYVFGNYITLTNLDETDYARIINQRITPLYVSLHTTDNALRQKIMRP
                     SKQVDALSVLKKLSRHGIGFHIQIVCIPGINDGEALKQTLKDLHDPEINCLSIGIVPV
                     GLTKYRKNLDAIIPYNRQTATEILALIDEARKEYNSTIIYPADEFYILAERDIPDEDF
                     YADYPQLENGIGMLRLTIQTYKQKKRGLLKELRKKPVNYLMISSVCAQNVITRIAEDL
                     NKRLENQFIRVQVIHNDFFGPDISVCGLITYSDLWQQLAPQAGETIILPASIFNNEGE
                     TLDGKDHLTFQETWKNPILLIDQFFEDWDYL"
     gene            complement(39820..40941)
                     /locus_tag="PLW08_01250"
     CDS             complement(39820..40941)
                     /locus_tag="PLW08_01250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPL51266.1"
                     /translation="MKKKFFVFFALLTCLGMLLGNITIQIKGEAKPVNLQETTLSSVS
                     YVALKDFSAIFKAISKEDRSDNRLYLNLYDEQFIFLENSPYYTLKTVSYNMHYPLLRK
                     GESLYLPSVFVTEQLKTHFPSLIQRKGSTLQIAKPIDNSVKTIVLDPGHGGKDPGAIG
                     KKLKANEKDINLAVALKLKNLLEKELGVNVLLTREDDRFVSLYDRTRFANEKRADLFI
                     SLHSNSSKSTTSRGIETYYLSTAQTSDARAVEAMENAVVERFEGGSEAKKKYDDLSFI
                     LSDLAQTEHLENSNNMAFNVQKNLIAGTQSIDRGVKQANFYVLRGAFMPSILVEMGFI
                     SHPEEEQLLVNEEYQDRLARTIFEGIKRFKFRYDRIRNT"
     gene            complement(40938..41882)
                     /locus_tag="PLW08_01255"
     CDS             complement(40938..41882)
                     /locus_tag="PLW08_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL51267.1"
                     /translation="MSYSQMFSCNICPRNCGIDRTKNKGYCGVTDKLRINLATLHFGE
                     EPCFSGTRGSGTIFFAGCNLRCVYCQNYEISTLCWGKDISVEELIRLMLKLQEEGAHN
                     INLVTPTHFTLQLREAIIQAKEKGLTIPVLWNSSAYEKVETLQLLTGLVDIYLPDFKY
                     AHKVYAQKYSAAPDYPAVAISAIKEMFSQVELLKLDEKGIAQKGLLLRMLVLPNKLAG
                     CKENLYTLTEELGTELTLSLMGQYYPAGKAKNYKELTRGITLSEYFEVVDTAVELGFT
                     KLYTQEISSSDTWTPNFSPAPEEINPASEFNPQLELTL"
     gene            complement(41883..42284)
                     /gene="cdd"
                     /locus_tag="PLW08_01260"
     CDS             complement(41883..42284)
                     /gene="cdd"
                     /locus_tag="PLW08_01260"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPL51268.1"
                     /translation="MVELNLSEKELLEAAKKAAENSYSPYSHYKVGCAVKTKDGHIFT
                     GCNVENASYSLTICAERNAIFKAISEGHRTFSEMAVYVDSEESFPPCGACRQVIYEFA
                     PEIEIIYANRKAIHKALITELFPSAFTLKKD"
     gene            complement(42298..43347)
                     /locus_tag="PLW08_01265"
     CDS             complement(42298..43347)
                     /locus_tag="PLW08_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPL51269.1"
                     /translation="MIIIGLILIWLAIKHKYEPTLLLPIGFGTILANIPFSAAVGEHS
                     PLGILFKAGIDTELFPLLIFIAIGAMIDFTPLLKNPFMLLFGAAAQLGIFVTIVLAAG
                     LGFEIKEAASIGIIGAADGPTSIYVANRFAPNLLGAISVAAYSYMALVPIIQPPVIRL
                     LTTKKERLIRMDYHSGDVPQIVKIMFPIAVTIIAGIFVPAALALIGFLMFGNLIRESG
                     VLEGLSKTAQNELGNLVTILLGLTIASKMQGELFLVPQTLLILALGLVAFIFDTAGGV
                     LFAKLLNVFKKEKINPMIGACGISAFPMSARVIHQMGLKEDPFNFLLMPAVSVNVGGQ
                     IGSVIAGGLILALLA"
     assembly_gap    43467..43566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            43663..44223
                     /locus_tag="PLW08_01270"
     CDS             43663..44223
                     /locus_tag="PLW08_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51270.1"
                     /translation="MRNIVLILLVALLLTGCDLFSVRDSEPPAAQIPWIDFTTEVDSV
                     LLNLEYSYEYPQNAVNYARLFSSDYVFHFAPQDVSDFSIEQTWTRVQEQDMIQVLHNR
                     YKNMQVTLEKVDDQDQIESAQAKIYRQYNITGISRNEGKEVLTLAEGNMELHLKKEYG
                     YWYITKWYDYRSSNVPTWGKLKYENH"
     gene            44210..44785
                     /locus_tag="PLW08_01275"
     CDS             44210..44785
                     /locus_tag="PLW08_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51271.1"
                     /translation="MRTISIILLFLTLLIGSCNNPFCPELYDVSNEEIQNRTPEELLQ
                     NLERAYTERNINIYKSLLHPDFRFELLASEVSLIGIDMNNDGMRDSWWGYAQEVEYTD
                     RMFNKGSSDGTYPPPDDIKLRLQIPPSERWENDPEVGHEDWIIIPCNFDLILSYQESN
                     SSITANGIARFYLRPVGNGWKIAIWRDESNI"
     gene            44782..45198
                     /gene="ruvX"
                     /locus_tag="PLW08_01280"
     CDS             44782..45198
                     /gene="ruvX"
                     /locus_tag="PLW08_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPL51272.1"
                     /translation="MSSKGRILALDYGEKRIGIALSDPNRIFSKPLCVLSNKGFEQLV
                     EELKKLIALHQVTLLIIGIPYAIDGSITPKTEECQQIMNRLKDVLSIPVEGFDERYST
                     WEANEELKKMGYNWRQAKEIKDAMSAAIILKEFLNQ"
     gene            45195..46178
                     /gene="mltG"
                     /locus_tag="PLW08_01285"
     CDS             45195..46178
                     /gene="mltG"
                     /locus_tag="PLW08_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPL51273.1"
                     /translation="MKKAKPFLIGLAILLCLLILVIVYLVFVPLHSEERIVRIKQGDN
                     ARIIAAKLSEAGIIRSKTMFIILTKIRKADRNLKPGSYIFGGNTYLWQTVSRLYKGQN
                     ESITITFPEGLSLYKTLKKIDASGLATYEELHKAATNPLLVKKLTGFDALSLEGFLYP
                     ETYRFPIEISPDSILAIPAGEFFRRLQKEGIDPFAVPDFYDKLILASIVEKEAGDESE
                     KEIIAGLFLNRMRQQMALQSCPTIDYILEPKGIKRSVLTYADTQINSPYNTYLYQGLP
                     PTPICNPSITTIKAVLNAKPNSYLYFFSDRQGKNVFSKTYEEHLAKQRTML"
     gene            46456..47277
                     /locus_tag="PLW08_01290"
     CDS             46456..47277
                     /locus_tag="PLW08_01290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51274.1"
                     /translation="MLGPYEINDIYKTDCVEGLKKLPENCIDLCVSSPPYDGIRDYHG
                     FSLDLHNVGIELYRVMKEGGIAVMVMQDQTRNFAKSLTTFRTAIDWCDNAGFRLFETL
                     IYRKYGAEGGWWKTRFRVDHEFMLVFLKGQRPQYFDKEPLKIPSKHGGKTLTGGGTRL
                     TNGIRIPTRPILINEMKCRGTVWEYLTAGDGSRLKHKHPATFPDKLPYDFIQCFCPPE
                     GIVLDIFMGSGTTALAAIELNRNFLGFEISEEYVKLAKQRIAIESKKDEDQLMFL"
     gene            complement(47300..48274)
                     /locus_tag="PLW08_01295"
     CDS             complement(47300..48274)
                     /locus_tag="PLW08_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51275.1"
                     /translation="MENNLDVLNYQELIKKYSWILERDHNCILSPDSDGLLCGLFMSN
                     YLNWKIVGFYDGKILIKDKKIDLNECIFLDMEIFRDFIRSAGHHIVLYSQRAIPELWT
                     NLNQCIQPNLLRGYYGQTHFKNKYPLAMIHLLIGILDNQEKISIETESICPLLFTDGT
                     FKNLFNYPENCLSWLHYLGADRKSSALHKIFFNECYTITSLMIALKELFKVISQDDYS
                     DKIKISTREGKIDGLQKDNSFFRFDDNTWMKTENFLKYLSAKTKWNYIQDKWIKSDFN
                     VFQFTKKSNKARVGIFRQILSENPLSMAQTSGNLIEYTIDPHNIFKNI"
     gene            48506..49072
                     /locus_tag="PLW08_01300"
     CDS             48506..49072
                     /locus_tag="PLW08_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51276.1"
                     /translation="MKLHKMMLLLVLVAVIVMIASCSSNKKANIKPQGEEVYRPAWWA
                     TQPEQDYVCTYGQATKVSENLSVEAARANALQEAAQYVEVLVNSMLKNYEEEAGVKDP
                     QVLALTQNVVKAVSSAKFTGVLMGKIETRKVNEPDGVRYKTWIQLKIPKDEINRKLAD
                     SIRNEEALYNQFKASQAFQELDKAVEKY"
     gene            49589..>50507
                     /locus_tag="PLW08_01305"
     CDS             49589..>50507
                     /locus_tag="PLW08_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPL51277.1"
                     /translation="MLKIGLNQEMVSRARKAAFQITNAFTWLFEGYTTVATERTVLRL
                     MGIDGALKDGKPLPNVVVDHLVENSALQKGAAIWVANAMLKKGLTAQQVAEEVAYKGL
                     DITQIPLASGEAIWETLNPLVDKMLGHITAQKQKREELLAKYQPRPQPVIYVIVATGN
                     IYEDVVQAKAAAREGADAIAVIRSTAQSLLDYVPFGATTVGFGGTFATQENFKIMRKA
                     LDEVAEEENRYIYLTNYASGLCMPEIAAMAAMERLDMMLNDAMYGILFRDINPKRTLL
                     DQYFARMINAYAGIEIQTGEDNYLTTSDAV"
BASE COUNT        15547 a         9064 c        10107 g        15589 t
ORIGIN      
        1 tttttacttg acaggttgta aacactattt acaggtgcac tgtaaacaaa attccaagag
       61 gggtgaaaaa aaatggcaga gaacataacc caacagctgg atgcatatat aacaaacaag
      121 atagcgcttt ccaaggcaat gggttactta agtgacagtg caaagagtca tctaattgaa
      181 atagtaggca aatcgggttc tggtaaaagt cattttgttc agcccttaat ggaaagtgtg
      241 gaagatatat atgcgaagcg gatatatttt tcccctcatc cccttatgtt aaatcatttt
      301 tcagagctta tgcaggtgat tacagaggta acggaagaag acctgtatgg ttattatcgg
      361 gaatttcgtg ataatatagg ttccgtaaat aaatacgatt ttttctttta tattacggag
      421 cgtttaaacg cgatggaact gtttcagcca tttctgctgg tgattgatga ttgtgatatt
      481 ttagataatt atacccgtga ttatttacaa tatctggttc aatatttacc agatacggga
      541 attcagatag tagtttttac gcaaacgcgt ttatttcctt ttgcggaatt ggacaatata
      601 cccacattag gagtggagga atgtcagcgg cttttacaat taacctttcc ggagacggat
      661 tttacaggta gcagtgaaag cgaaatattt cagaaaataa cttccggcaa cctgatgatc
      721 atagaaaagg tcttgaagga gatgaagagc caaaagccaa aggggaagtt taatttggga
      781 ccttatctgg aaaagacctt tgatgctgaa cagatttatt atgaaacgct ggaaaccctt
      841 agttcctcac agaaagaaat tctaactgcg atgtatgtat tggatggatt tgatgccagt
      901 gccttagcta aagcgttggg caatccccaa aatataaaga cggatttggt tactttatat
      961 aatttgaatt tgatttccaa accggaaaag ggattgtcca ttcagaagaa gaaggcattt
     1021 tcggaatggc tgaataacaa tttgaaggag ttcaaaggca ctttattgaa tcagctgatg
     1081 gaatatctgc aaaaggagaa aaagcatccc cagattttgg tttctctgca gatgttaaat
     1141 aaaaaataca attccgctct ctttggtaaa gtaatttccg agctatctca gatcaacgat
     1201 tccgcaagtg tgttaaggtt agaagaatat gttttggcgc atactaaaag tcccgcggac
     1261 aaattagcca gcacgcaaag aattgctgaa gcatattcag tgctaaatca gaaagacaaa
     1321 gcgatagatc attttcgtca ggcattgcat atttctacgg aaaacaatat tcctgcagag
     1381 caaatcgttt ataaacttgc cagtgatctc tatgccgtaa attcttccgc ttttgctttg
     1441 gaaattatca aacagtattc tccggctacg atagacccct attggaaagt aaaaattctc
     1501 ctgttaaaag ctgatattca aacggaaagt gaagcattta atgaagcatt caacacattg
     1561 gatattgcca gccattcttc aaacagcata actaatcagc aacaaagaca cagcgttcaa
     1621 gccgaagcca aaaaaatacg cgggaaaata cactattaca taaatgaatg ggaacaggcg
     1681 gaagaggttt tcaacgaagc agaaacgctt tattctcttg ccaaagatca cagcggttta
     1741 gctgctatct ataacaattt gggtgttttg tatatgtgtc agggtttttg ggaaaagagc
     1801 gaaacctatt ttctgaaaag tttggcactg gaaaaacaat attttaatct gaatggcatt
     1861 tcggtatgtt ataataactt aggcggtttg atggatgata aaggtgatcc ggcaaaatca
     1921 ctttattacc tggaagaggc acttaaaata cagaaacttt tggctgaacc ctataatatt
     1981 actaatatct ataataacat aggtgttacg ctaatggacc acggtgacta tgacagggct
     2041 gaagatgcct taaaaaagtc gctggatatg gctaatgaat ttggttttca gcgtaatacg
     2101 attgccactt taaataatct gggcggttta tatttcaaga aaggtgactg ggcagaatcc
     2161 atttcttact atgaaaaagc cattaaactc tctcaggaaa gcggttttaa cgaaggtcta
     2221 ttgcgcagtt ttaataactt gggcgaggtt tatgaaaaac aggatgaact gaacctggct
     2281 tatgatcttt atattaaagg tctggaattg cttcctgccg tttcggatga ttatattaag
     2341 gcagaattgt atggcaatct gggaagtgtt tttactaagc tgcataaatt taaggatgcc
     2401 tatcgttatc tggttgaaag cttggatttc tttaaaaaca ttgctgcccg ggataaaatt
     2461 attgagggtt atctgaaaca ggcatattac tttatcctaa cccgcaatat tgaaagtgcc
     2521 gattattatc tggcggaagc tcaaaaactg gctattgaac aaaatctgcc ttttgaaata
     2581 gggcgttctt tatacctgcg tgctttgctg gaaaggaaaa atttggaatc cgcaaaggat
     2641 tatctgaatg aggcaattaa acattttgta gaagcaggta gtaactatga actttcttta
     2701 gccaattatg aacttgccgg tgtgcttttg gatatggaag aatgggaaca ggcaatggaa
     2761 attttgaaaa ataaccgcaa aataattgaa agatacggct ctattaaatt actggaacaa
     2821 aatgacatcc tgatgcaaaa aatatcccgt gaatattctt cgcaaatgga agaagtaaaa
     2881 tttgaagaaa acctgctgaa ccagttctat gaaattaccc aaaacctgaa tacgataacc
     2941 gatttggatg tgctgattga acaatccctt tccagtttgg tggaaattgc caatgccgat
     3001 ggcggaatgc ttttactgca tcctaatccg agtttgccgg atgcctggga atatagaatt
     3061 tatcataatt tttctcctga agataagtat cacgaccaat ttcagaatat ttgttatgaa
     3121 gtgtatcaga ataataagct ggaaaacatc aaacagccgc attttgctcc cagttataat
     3181 aatattttag ctctgcctct ttccattcgt aaaaatacta tgggagtggt tttgctcttt
     3241 tgtaaaagcg ggtccaaata tttttccgaa aggattatca atttgctttc cgctctttcc
     3301 aatcaaatta tcgtaattat agaaaacatc ctcagtgcca atctggaaaa aagccataat
     3361 gttatccgcg aacagcttag ctccaataat atctattcca atataatagg taaaagcccg
     3421 gagatgatga agatttttga gattattgaa aaggtgaaag atacgcctac tacagtgctt
     3481 ttagaaggtc ccagcggaac aggtaaagaa ttgatagcca gagcattaca ctatagcagt
     3541 aacagaagaa ataaggcatt tgtagctcaa tattgcggtg ccttgcccga gaccttactg
     3601 gaaagcgaac tttttggtca tgtaaagggt tcttttacag gtgctgctta tgataaaaag
     3661 ggtttatttg aaatagctga tggtggaact ttctttctgg atgaaatttc cgatatcagc
     3721 cagtccacac agtccaaact gctgcgtttt ttgcaagaag gagaaattaa aagagttgga
     3781 gctacaaaaa cggaaaaggt tgatgtgcga gttgtttgcg ctaccaatac acctttaatg
     3841 gaaagagtta aaagcggaga attccgttta gacctctatt accgtttaaa tgttatcaga
     3901 attgatgttc cgccgctgaa aaacagaacg ggtgatattc ctcttttggc aatacacttt
     3961 ctggataaat acaataaacg aatgaataaa aatgttatgg gatttacagc tgaagcaatg
     4021 aaggttttgg aacaatatga ctggcctgga aatgtccgtc agctggaaaa tgaaattgaa
     4081 agagcagtaa ctttggctga acccaatact ttcattaaac ccagtgattt tagtgaagaa
     4141 gtttaccgtc atattgaata cagcaaaaca atcgctatgc tttcgggaaa gaaaaactta
     4201 aaggatgcca ttgaagaact ggaacgcaaa ttgatttctc aatccctgga aaaatttcag
     4261 tggaatcaaa cccaagcagc taaagaattg gggctttcgc gtcagggctt aattaaaaag
     4321 atgcagcgtt ataatcttta ccgcgaagag gattaacgaa taggataaaa tgtggaaagt
     4381 ataaacaacg aagtaaaaaa cacggagaat tttaacaacc tgcctttgga gcaattggaa
     4441 tttgtagcta tagatattga aactaccggc ttaaattcgg aatgggatga aattattcag
     4501 atagctggta tgcgctttaa gggtgaagaa gttgttaatc agtttgtcag tttggttaag
     4561 cccaaaggga aagtgcccaa atttattgag catttaactc atatttcgcc tcaggaacta
     4621 aaaacggcac ctcctataaa agaggtgctg aaggattttt gtaattttat tggcgatagc
     4681 attttggtgg gacataatat tggttttgat tttaactttc tcaatcaatt tatcgtggat
     4741 aatgggggtt tcccgatcag caatatgcat tgggatacag cagaaatagg acgtatttat
     4801 ttacctttta caactgatca taaattaagc accctggttt cttttttcgg aattgaacta
     4861 atgaatgctc atcaagcaga tgcagatgcc ctggcaacag gaaagctctt taatgcctta
     4921 accaattata ttttaaatca ttatggctat atgctgaatg cccggttaat ggatttagct
     4981 atgcaggcaa atctgaaaag cggattgtat gaatatttca aatggattgt gagacaacag
     5041 cgttcatcag ctctttccgg taaaaaaacg gttcctgtag tcaatgaact gaacaatgtt
     5101 attgagcata ttgctgcagg ttccataaaa agcattcctg aggtctttgc agaagatgga
     5161 atttttgcct ccaaatttcc caattttgaa tttcgttccg gacaaatgac tatggcagaa
     5221 gaggttgccg atgcttttag caaacaggat tttttggtgg tagaagcagg aacgggagtt
     5281 ggaaaatcct ttgcttatct ggttccggca attgatttca gtaaccggaa taagagcaaa
     5341 gtaattgttt ctaccaatac taaaaatctg caggaacagc tattcttcaa agatttaccg
     5401 caattgaaaa aaatattgcc cttgcctttc caagctgtat tagttaaagg tagggaaaat
     5461 tatatctgcg aaagaaggtg gcaggaactt ttacaggaac aaacaagagg cataacttct
     5521 tacgatgcgt atggtttact aaatcttctg gtttggaaag aacatacgaa tacaggtgat
     5581 gtttccgaaa attcttcttt tgataaaaac cgcttcggag ttttatggca taaaatatgt
     5641 tccgatcgct atctttgtgg aggcagaaag tgtcccttgt tccatagctg ttatgtaatg
     5701 aaattgcgta agaatataga gacagcatct attgtagttg ccaatcattc cttgctgctg
     5761 gcagatgctc aaatggataa ttccacttta ggtgaatatc aatatctggt aattgatgaa
     5821 gcgcataatc tgatgtccgc tgcttcccaa aatctgggtt ttactttaag ttatgttgac
     5881 ctcaacagct tatttaatca gcttgtttat acttccagga aatattccgg gggctttctt
     5941 tcccaaatta tgcaggtatt gcagaagagt atgttaacca ctgacaaaaa gacgcatata
     6001 gaaacccttt gcaccaaact gacaggggaa attgacagtc agaggcaggt gataacaaag
     6061 cttttcagtt tagcggaaga ccattgtgtt gaagcaaaaa gctataataa attgcggata
     6121 aaggacatca atgcttttga agatatctat acccttctga aggaattatc tcttgctttc
     6181 aaggatattt tgaaagacct tactgccctg gataatgttt tcagcacttt aaacagcaat
     6241 caagtgaagg atttggatgt tctgaaagat aatttgaagg gttatttatc ccgctatatg
     6301 gaaacggaag agataatttt acaacttctt aatcccgatt tggataatta tgctttatgg
     6361 attgaaaacg atcccatttc ggaacgcaat gttcctatca gcactttttg ttatgcacct
     6421 gtggaagttg gagaacatct taaccgtttg ctttataaaa aaatcccctg tattattttc
     6481 acttctgcta ctttatctat cagaggttct ttcaagtttt tcctcaatca atcgggcttg
     6541 agtttggtaa cagaaaagaa tgttaagcag agtattgtgg aatcaccttt tgattatgat
     6601 aagcagtcct tattgttaat tggcagtttt cttcctgagc ataaggataa attcttccaa
     6661 agtcaggcat tgggttgttt ggaacaaata ttgactacta caaatgtagg aacgatggca
     6721 ctttttactt cctataagga cttggatgct gtttacaatc atcttagcga tacccttttt
     6781 catctcaatc gtccattttt tgctcaggga aagggtgtaa gcagaaattc ccttttggat
     6841 gaattcaaga agagcaaaaa tgccgtgtta ttgggaactt cttctttttg ggaaggggtg
     6901 gatattcaag gggattcact ttctctattg attttataca aaattccttt tcaagtgcct
     6961 tccgagccat tggtagaagc acttatagat aaactggaac gggaaaataa ggattccttt
     7021 atgcatctga tgttgcccaa tgctttattg cgtttaagac agggttttgg acgcttaatc
     7081 cgcagtaaaa cagaccgggg agtagttctt attatggatt cccgtgtttc caaaaagaaa
     7141 tatggcattt actttaaaca aatattacca ggacgctgta ttgaattgaa agacgaacaa
     7201 catctcattt ctgaaatcgg taaatttttt aatcctttac ctgccaatat ttgagtatgg
     7261 atacttttct tttgttacgg aaagaatacc ctcaactaat tgatgagggc attgtgcata
     7321 tagaagttcc cttaaaagaa cattgcagtt ttaacatagg gggtcccgct gaggtttttt
     7381 gcactcctta tacccaaaaa caattagtta ctctcttaaa gttttgcctt gagcataata
     7441 ttccttactt tattttaggg aagggctcta atttactcat atcggataag ggagtaaagg
     7501 gaatagttat cagcacggaa cggtttgata aaatcacttt aggtaagcac tatttgtctg
     7561 ctttctgtgg agttactctt aaggacttaa gtgattttgc ctgtgagaat ggattaagcg
     7621 gattggaatt tgccagtgga attccgggct ctgtaggagg agctgttttt atgaatgccg
     7681 gtgcctacgg aaacgagatc aaagatgttc tttacagcag tagagctatt ttgcctaccc
     7741 aagaaaagct caattctcca aaccctgttt tatacctgaa ggtgtcagaa cataattttt
     7801 cctatcggca tagcatcttt caggatttgg gcttaattca tctttcctct ctgtttattc
     7861 tgaaaaaaga taaaccggaa aatatccgcc agcgaatgca ggaattacaa caaaaacgac
     7921 aggataaaca accaatggat tttcccagtg ccggtagcgt ttttaaacga ccggaaggtt
     7981 tttttaccgg aaaattaatt gaggaatgcg gattgaaggg ctttcggatt ggtgatgcag
     8041 ctatttcgga aaagcattgc ggttttattg ttaatcttgg ttctgctact gctaaagatg
     8101 tttttcagct tattaagcat attcaaaaag ttgtttatga acgctatggt gttcatctgc
     8161 aaacggaaat tcgtattttg ggagaaatat gaaaagccgg caaataattt tgcctttgct
     8221 tttttttgtg cttctggttt tgctctggca atttatcagc actaaaggag ttatcgcttt
     8281 ttgggttgtt ccttcgccgc aacaggtgat tcaggttttt cttgattatc caggtttgat
     8341 ttggcatcac ttaaagccaa ctttgataga ggcaattagc ggacttttaa tcagtattgt
     8401 tctgggctct gtaacggcta ttgcaatgaa tttatcccgt cttatcaaac aaattatcta
     8461 tccttatctg attatttccc aaacagttcc tattatagct gtagcacctt tacttataat
     8521 ttggtttggt tatggcatca gtgctaaaat ttttgctgta gtgctgatgt gcttttttcc
     8581 tattgcctta ggactttatg acgggttcag gagtgtgtcc atagaccaaa ttcgtctttt
     8641 aacttcaatg ggagcaacac cctataagat atttagatta ttaaaaattc cttccgcttt
     8701 gcccaatttt tttacgggac ttaaactatc tgcaacttat agcgtgatgg gagctgtgat
     8761 aggtgaatgg ttaggtggaa cttccggctt gggaatttat atgaccagag ctacaaagtc
     8821 ctatcaaaca gctcatattt ttgccgtgat tattgttatc gtggctgtca gtttagcttt
     8881 gttcggaatc gtggctttgc tggataggat tttgctgaaa tggaaatatc atcctattga
     8941 tgaatatctg gaacccgaaa aaaaataacc tctcacagat aaaaataagt gcacgcagat
     9001 ttcgcagatt acgcagattt ttttatttat aattatcctt atattgaaat acagccgatc
     9061 cggtcgatcc ggttatctga gttctcttag tcattcttca tttttcattt ttatttgtgc
     9121 ttcttggctt ggaacttgga cgaaatcgcc gggttggaag tttccggaaa agcggacgcg
     9181 gacatagtga tcgctcaaaa attcgcagta accgttggtg tttttttcca caattccgcg
     9241 taacgggata ttattttcca ttagggattt agtataggcg gaagtcagtt cgcgggaaat
     9301 ttggctgagt tcattggctc tgcggttttt aatggtttta gagatttggt taggaaaatc
     9361 aaaagcaggg gttccttttc ttttagaaaa agtgaaaata tgaagataag ccaggggtaa
     9421 agagcgcaaa aattcacagg tctggttatg ttcttcttcc gtttcaccag gaaaaccggt
     9481 tattacatcc attccgattg ccggataggg aatgcgttca caaatcttaa ttattagttg
     9541 gttaattgtg gtggaggtgt agtggcgtct catccttttt agcccagaat cagcaccaca
     9601 ttgcaacggt atatgataat gcgggcaaag cttgggaatt aagggaagag tgttaattag
     9661 tttatcatta aacagttgcg gttcaacgga gctaaggcga atcaattcca agccctgcaa
     9721 ttcgtttaag cggagcaaaa catcggttaa atcgttattt ccatctttgt atagtcctaa
     9781 gttaattcct gatagcacaa tttccttata accgttttcc acaaataact tggcttgctg
     9841 aataacttgt tccaaagttg ccgacctgct tttgcccctt ccataaggaa ctgcacaata
     9901 agcacagtta aaatcgcagc cgtcttgaat aatttgaaag gcgcgtgtgc ggttatacat
     9961 tttagcagtt ggcttataga caaattcctt tgcctgcata atatccataa attcgtagtt
    10021 gccaccggca aggatatccg caatattcag tttctgctga ttatctataa tccagtcaat
    10081 attgcccatt ttggcaattt cttccggata gcgttgagca aagcagccgg ttacaacaat
    10141 tttggcaaag gggttttgcg ctttttgaga taatgcctga cggattaaat agcggctttt
    10201 atagtcggtt cggttcgtta cagtgcaggt attgatgata taaatatccg cttcttcctg
    10261 ccagggaact aacttaaagt tgttgaactg gtctaaaatt atggaggact cggcttgatt
    10321 ggctttgcaa ccaagggtag caatagcgat gcgaatcacg attttaacaa gaattgacag
    10381 atggcaggtg cataaagttt gtgttcaatt tccagcactt tttcggcaat ttcttccgga
    10441 cttttacagg aactgatatc cactttttgc tgagctacaa tttttccctt atcatattgg
    10501 gaattaacaa gatgaatagt aacaccggaa aatttgtcac aggaagcaaa aaccgcttta
    10561 tgcacagcca ttccatacat acctttgcct ccgtattggg gcaataaggc aggatggatg
    10621 tttaaaatgg ggacttgaac ttcctcaata aaattctccg agagctgttt taaaaaaccc
    10681 gctaaggcaa tcagttctat gttatgttgt tggcaaaggt ttattgcctg ttgttcaaaa
    10741 agttgcatat tgcgggtaga aataatgtgg taattcaatc ctttttcttc agccaattga
    10801 actgcaggtg catctttgcg ggtgaaaatg accaaagcga cttcaatggg taacttattc
    10861 tgtgtaaaat agttatgcaa tgcacacaaa tttgagcccc gacttaatcc gctgcaaaaa
    10921 acagctattt tatgggttgc agatgcaggt ctttccattg ctgttcatct acttcggaag
    10981 gtgcctggct cattaagtcc gttgccttca atgttttagg gaaagcaata acatctcgga
    11041 tggattcagc tcctgtcata atcataacta atctatccaa accaggagct attcctccgt
    11101 gtggtggagt tccatatttt agtgcttcca gaaagaaccc gaaacgcttc tgcagttctt
    11161 cttcactaaa accaagaata tcaaatattt tcttttggat gtcataacga tgacacctga
    11221 tactgccgct ggaaagttca actccattgc agacaaggtc ataaagttgt ccaataatat
    11281 caccgatttt ttcgggaata tccagatagg gaatatgttc ttctttgggt aaagtaaaca
    11341 tatgatgagc tggttcccat ttacctgttt ccagattttt agtaaagagg ggaaaatccg
    11401 ttatccagca aaaggcaaaa ctgttttcgg gaatgagatt taattgggga gccaagaaat
    11461 tacgaattcc tgctaaaacc ttgcttacca tttcataatt atctgcggca aaggcaagaa
    11521 ggtcattttt ttgagcttgg gtagcttgaa taattgcctc tgcttcttca gggctaagga
    11581 atttactgat gccaccttcc aaaccgtttt ccgttacttt ggcaaaagca attccttgac
    11641 ctcccaagtg cttagcaagt tctaccagtt catcctgttg ctttctgctg aaatcagctg
    11701 ctttaggaat ggcgattgct ttaactactc ctccgttttt cagggcggaa gaaaagacat
    11761 taaaaccact atttttcact atgttagaaa tgtcaaataa cttcatttca aaacgaatat
    11821 cgggtttatc acagccataa ttctccattg cttccgcata aggaataaca ggaaaaggaa
    11881 tgcttaaatt atatcccaag acctcctgaa agagtttggc aaaaaggcgt tctataagat
    11941 caaagatttg cgcttgagaa acgaaactca gctctatatc caattgcgta aattcgggct
    12001 gtctatctgc tcttaaatcc tcatcccgaa agcatctggc tatttgatag tatctatcca
    12061 agccggcaat cattaagagc tgtttaaaca tttgagggga ttgcggtaag gcataaaatt
    12121 ttcccggttg cattctactg ggaacaagat aatcccttgc tccttcagga gtgcttttca
    12181 tcaggatggg agtttcaatt tcataaaaac cctcattatt gagaaattcc cgtataattt
    12241 gcattatgcg gtgcctggtg ataattatct tttgcagttt gggacgacgc aaatccaaat
    12301 agcgataagt aagccgcaaa tcctcttccg ccatagcgac ttcactaaat tgaacaggaa
    12361 ggggttgtgc atcatttaaa atataaaatt cctccgcttc cagttcaatt ttgccggtgg
    12421 gaagattggg attaatgttg ggttcggtac gcgcacaaat ttttccgcgg acagcaatta
    12481 catattcatt acgaactttt tccgctttgg caaaaatctc tggtttttcg ggacggataa
    12541 caacttgcag aataccttga acatccctca ggtctataaa aattaaaccg cccaggtcgc
    12601 ggcgtttatt tacccaaccc ataacagtta cttcttgtcc aatgtgctcc gtattcaaat
    12661 ttccgcaata atgagtgcgg gttaaatttc ccaggttatc aagcattgtg ttttcttttt
    12721 ctcgctttat tagtttaatt tgattgcctc aaagagctta tctattttct tcacattttc
    12781 gccaagagca ttaatcatat agcatttttg gagatattcc tcgcttggat agataagtgc
    12841 attattttta attgtatcag gtaacaaatt gtttgccgcc ttattgggag tggcatagcg
    12901 gcaatattca gtattgcgtt taccgatttc cgcatttagg agaaaatcaa tgaatttata
    12961 ggcaagttct ttatttttag aggatttaag cataacgata ttatccatcc ataagctggt
    13021 gccttccacg gggagaaaga atttaatatc gggattgttt tccataacct gtaaagcatc
    13081 tccgttatat gcttgagcca gccatgttgt tccatcggga acttcgtttt tatagctgtc
    13141 agaatcaaac tgcgtaatat ttttatccca ttcggcaagc gttttttctg cagcagctaa
    13201 tgcttcagca cttgtatcat taagatcgta gccattgtag attaatgcgg caccaacaac
    13261 ttcgcgtgca tcatccagca tcgtaacttt gtttttgccg gtgaaaaaat tatcggctaa
    13321 gatattccag ctttgggatt taacaacgct ttcaggaaca taacgctgat tataaataat
    13381 tccggttaag ccccaaaaat agggtaggga atatttattg ccagggtcaa aactggttgc
    13441 cttcagcaaa agattgttat ccagattgcg gtaatttgga attttggtta aatccaacgg
    13501 ttccagtaaa cccgcattac acataatagt tacatgatca ccactgggaa aaacaagatc
    13561 aaatgcttcg cgggaactca taattttggt gagcatattt tcgttggaat cgtaagtgct
    13621 atatttgata gtgcatttat tctgctgttc aaattccttg atgaggtcgg gatcaatata
    13681 atcactccag ttgaagatat acagaaccgg cttattttta ctacaggaac cgcaaaaaaa
    13741 cagcagaact atgaccatta ttaggactat gatatttcct tttttcatct ttctctcctt
    13801 tttttctctc acagattaca cagataacac agataaaaga ttagcacacg gatttaacgg
    13861 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactttcg cttgttcaat
    13981 cccttcctgt aactgcacac tctgcaataa aatccagcca atcacaaaga aaaaaatcaa
    14041 ggataaaacg gaataacgaa tatcccctgt ctgatgtgct atccatccat ataaaattgg
    14101 accaataata gaagataagc gtcctgtcaa ggtataaaat ccgaaaaatt ctgcctgtct
    14161 atccctcgga gttaaaagag aaagcatagt tctactattt gcctgactac tgccaattgc
    14221 caaaccggca accagaccga caaaataata ttcactggca ctgctgcaaa aaaatgccca
    14281 gataacaacg ccaatccaaa ttataaggga tatactcaag gaatttttta cattccattt
    14341 atccgtaagc caaccgaaaa atgccgctcc caaaatggaa gtaaattgtg ccagaatgaa
    14401 ataggttatc atctgctgag ttgtcatccc aaacctttcc gctccataaa tagaagcaaa
    14461 aacaatcact gtagtgatgc catcgttata aataaaatag ctgataatat atttcagcag
    14521 ttggggtaac ttggcaatgt tctttagtga ataagtaact ctttgccagg caatccgata
    14581 atagttactt ctttgagagg gacggtgaaa ttctttcaac caaaaaaaag tgaacaggga
    14641 aaataaaaac aggtgcaatg ccacagcagg aaaaaccaaa cgcacattga tttttaccaa
    14701 aactaaagct acaagcaggg aaactaaacc acctacatat cctaaagacc agccaaaacc
    14761 tgaaacctta ccgatatctt ctggctggca aatttcgggc aaaaaagcat cgtaaaatac
    14821 attagcacta ttgaaaccga aattggcaat aatgaaaaac aacattccta taaaaacatc
    14881 acctggctta acaaaataaa gcagagccgt aaaaattacg gttatatagc aattaataaa
    14941 aagcatccgt tttttggcac gggaataatc tgctacagct ccaaaaatag gtgctgaaat
    15001 agcaaccagg gtcatagaaa ttccgattgc tcttccccaa agcatttctc cataaccggc
    15061 aggaccgcct actacattat tgataaaata aacgctgtaa acgactgtaa caattatagt
    15121 tgtaaaggca gaattggcaa aatcatagag catccagccg ataatattac ggttgaattt
    15181 catttgtcta ttccttccaa cttctgaatt tgatagccct gccagatatt acagagatct
    15241 tctttgggac tggcaataaa aacctgataa cggttattga tgcaatttct tatttgttga
    15301 gaatgaaaag tatccaactc ggcaaaaata tcgtcaaaaa gcataatagg ttttatgccg
    15361 gtaactgttt caattaggcg tgcttgaata agcttaagaa taataactgc aatgcgtttt
    15421 tgcccttgcg aagcataagt aagcatttta cgacccttaa gcttaaattc gtaatcatcc
    15481 aaatgagctc cgactaaaga gcgttgtagt aatttttctc tttcttcaat agttgccagg
    15541 tgttttataa tttcttccgg agaactctcc aaaggcaatt ttaaagaagg tatataagcc
    15601 agagaaatgg cagtggaagc aggaaaaata tctttgaagg tctcctgaaa agcggtatta
    15661 acctgcttta aatagcgatt ccgataattc cagacctccg ctaaagaaga agcaaaagat
    15721 aaattccagc tggtaatttc tgcccgggaa tagtttcttt tcagcattgc atttctctgc
    15781 tgaacgatat gcaaaaaatg ccttaggaca ttgatgtaag gaggataaag ctgagaaatt
    15841 gccaggtcaa aatattgtcg gcgaaaacgc ggtgaaccac ttatcaataa atggtcttct
    15901 ggagcacaat aaataacttt taccacttca aaaagagaac ttaattggcg gataggcaat
    15961 tcgtcaatct ttaaaagttt atgctgatct gcataactta aagagatctt cttcggggtt
    16021 tgctggtcgc tgataaataa actctgaaca cggaaaaact gcccttcaaa attgagcagt
    16081 tcttcatcgt ggtgaaaacg aatggattta cctatactgc aataggcaat tgcctccagg
    16141 agattggttt ttccacagcc attgggacca attatcaaac agccctgagg gttgaaatca
    16201 aactcatttt ggcgatagct gcggaaattc tcaagttcaa ttttagccag gttcaaattt
    16261 ttatgaacgc aaaggcatca gcaggaaggt tatttcctga tcgggaaggg gtgtttcatt
    16321 gtaaatcatc atcgcgtctt tggaagttcc caatttgatg acaacttttt ccgtatctat
    16381 agcttccaga atggaaagca tatatttata gttgaatgaa attcctgtag aagagccagc
    16441 aaaagtaaag tcatccatat tttctttagc ttcaccggta tctctgttac tggtattcac
    16501 ttcaaaacga tcactatcca aatcaaaatg aatgcgtaaa ttgtcatctg gagccaccag
    16561 agctactctt tttatagcag taatgagggc ttgtctatca ataacaaatt tattgggcag
    16621 ttcggcagga aatgcttttt gataatcggg aaatttgtgt tccaaaacct gagaggaaat
    16681 aaaatattct ccatacaaaa acagaatccg gttatgttcc aaacctactt tcaattcctt
    16741 cacttcatca ttgaatattt tttgcagaaa cagaagggtc tttactggaa tcactttttc
    16801 cacataatat tgattctgtt ctgtaaaaat attcatttcc gggttatcat tcggttccaa
    16861 agaagataaa agggacgaat tcagaatttt tatttctgct actttgcgtc catcagttgc
    16921 tgccattata tgatgatcag gataaatctt ccagcaaaca ccattgagaa caggacggtt
    16981 tacatctgtg gaaacggcaa aataggtttt ggaaatcatt ctgctaaata actctgcatc
    17041 aatagttatg ggattattca gtttcggctc gggaataaca ggaaaaagag tgtgatcagc
    17101 aattaacaga ttaaaatcaa ctttgccgca ttgaatcatc agcaggtcgt cattacgcca
    17161 aagattgata actccatcgg gcatataatt aataatttca ttaaaatgat gagcagaaac
    17221 agcaactgtt ccaccttcag aaacggctgc aggaaactct acaacaacca taagttccaa
    17281 atcggtagta ctgattcgga ctttattgtt ttcttccgag acctcaataa ggtagttgtt
    17341 caatatcgga gatgtgtttt ttgtaggcac aatggatacc agatgttgaa tgcgggcaag
    17401 aaggtctttc ttttcaatcg ctaatctcat aataacctca ctggaataat ttactttttt
    17461 caagaaaaat ttactgtgtt atttagtcaa tcactttttt ggagaaaaga gattttacac
    17521 cgagaacacg aaaacaccga agtcacagaa gggaatttta atgcggaaat tcttgtttta
    17581 ggtaatatga gctacaaact ccttgacaga aagcaagcaa aatttatcaa gaagcagagt
    17641 gtattttaga aaataacaaa aaatgaggat tggattttgg aacccctgaa atctaaggca
    17701 ttagcagaaa atctggcaat tactaaagta gccgaaattg tgcttgatga tgaggcaaaa
    17761 tggttacttt ccttaacgga aggtaatttt ggcatcaatc aacgctgccg gaattttttg
    17821 gaagaactgt atcatcctta tgctaatccg gaagcagtag catcgctaat tcggcaaagt
    17881 attttaggtg atttatggtt ctatgttcaa ttgccggata aggaaaaaac cttaaccgtt
    17941 ataggggatt tgtatttact ggcggcaaaa aaatgcacta agcccatgca ggaaaagaga
    18001 attattattg aatatctgga tttcatttcg gctttatgtg acaaggaagg cattccggaa
    18061 agcgtttatg ccaagttact aaacgaactg gataactggc aaaaaaacag cagggagtta
    18121 tttattcctt taaatggtgc ctgcagaaaa tgtttaagca atctggtgca ggtatataaa
    18181 gacaacaata tttgtttcag gatgctgaaa gacctgttaa aagagggctt ggaattttgg
    18241 cagaaaacaa cagatataga gggctggttt gcaaaaatgg gagcggaaag caatttggat
    18301 gtaaaacaac tttcctcaac cttgggcaaa aaatattatc aaaaatggct taagcaactt
    18361 gaagaaattc aggattttgc atccctgaaa gatattccct ctttttccga tatagccgtt
    18421 ctccaaagag aagcgatttg tgaatttagc accttaacct tgagaataca atatatattt
    18481 tatcttttgg gtttacccgg aatggaggac ttgcgtaatc atctgctttg ggatttgaat
    18541 cgtcaattag cagatttgga aaaggacttg aaggaagatg aagtcccttt gatgttggat
    18601 tccatttttg agaccttata cgaatttaag gaaacacatc tttccattgt tttagattgt
    18661 attttaacta ttggcaaaac agtgctttct cataaaaata agaaacactg ccaacatctg
    18721 ctccctaaaa ttatggaact ggaatttacc cctcccggaa agattaaaat agataaggac
    18781 tggcagatgg aagtggataa aaatcatatc aaacatctca gaatgctgct ggaacttatt
    18841 gctattgatc ccgttatgtg taaagacctg cttgcctatt taattatcag tttaagccaa
    18901 aaaggggttt ttatttccga taccgatctc tttcagaaag atgtttccgc ctttttgaat
    18961 tccaatttta agcccctggt agtgcaaacc aaacacttgc tgaaaatgtt tcccgtcttt
    19021 ttcaatgaaa ttggagcgga aggtgaaata agggacataa gtacctctct ggatgaattg
    19081 ggacacagaa aagacctctt aatgcacttt ttacgcaaac agttacatac tgaaagcaat
    19141 aacacccatc tccttttaat tcagaaaatt ttgcagtatt ggactaattt ggattataga
    19201 gaactgcagg atatgattcc ggaagatgtt ctcatttatg tgatgcatcc ggatgaaatg
    19261 acaaaaaagc aaagtgcagt ggttaataag tttttacaaa agcataactt aactcctgaa
    19321 gagctgcttt ctctttcctg gcaaagggtt agtaaattat ttgaaaacga gccggaggac
    19381 ttttctttaa aacgactgaa acttctatgt tactcgcatt ttctgctgaa ggacaagtat
    19441 aatcttgatc cttatgatat tgtaaaattt ctaacccgtt acagctggtt tgatgctcac
    19501 gaacataatc gccttgccaa tagctttatg agaaaggatt atgacagttc aattaggcaa
    19561 ttgctttcct atatcggcaa aatgaacagc gttattttag acccacaacc tactactgcc
    19621 tgggaaaata tatattacaa aagacacatt gctgctggca ttccttctat gtatggaatg
    19681 tatcgtgaac cgaaactgga agcaatggga atgattttta gaatagaaaa tgtggttaaa
    19741 cggctttttg aacgcaatgt gcaacaattg aacctgagct atattaccgg caagaccctg
    19801 aaaagaatta tccggatatt ggaattatac gattatgcaa tgaaacagat tttggttaca
    19861 agtgatgctt acggttctgc tttgaatatg ttaagatcag ctcaatatct tagcaatctt
    19921 tccatagacc agtatctgga tattttcaag ttgctgaaag atagtgtggt ggaaattata
    19981 aacgaatact attatagatt ttacgaaggg caattgaggc aaattggaat gcgaatagca
    20041 gaaaaggaga aagggaaaaa aggtagggga actaaggaaa tagaaatttt tgccgaagaa
    20101 ttttatcgta atctgctttc taaatccttt ctggtgcagg gtttggataa ttttatcacc
    20161 cgaatcttgg aatccttatc cgagatgaaa aatcttttta agccggaaga gattcccaaa
    20221 gtaatgagct atgatcccga taaactcttt tttcacttaa ataaaaagaa ttctcgcata
    20281 gaaaatcaag tgcttttggg ttctaaagca tattttctga aacggatgta tcaatatgaa
    20341 tttccggttc ctccaggttt tgtgattact acagaccttt tccgtcatcg gaatattatt
    20401 aatacacatc ctgatatatc taacgaattg gataacttaa ttttgcagaa tttaaacatt
    20461 ttggaaaaga atacagggct tgttttaggc gatccggaaa gacctttatt gctttctgtc
    20521 cgttcaggag ctccaatgag tttgcccgga gcaatgaaca cttttttgaa tattggttta
    20581 actgatgaag ttacatataa actttcccgt cgtcctaatt atgggtggac tgcctgggat
    20641 tgttacagac ggttaattca aagctggggt atggcttttg gaattgaccg cgatgaattt
    20701 gatgccgtta tgctaaacta taagaaaaag tataatgttg agctgaaaac ccaattttca
    20761 gataggcaga tgcaggaaat ggtgcaggat tatcaaaaag tgctggctaa acataatatt
    20821 gaactggaac agcaaccctt tatgcaactt tataaagcaa tttccaatgt tctggatagc
    20881 tggaatacgg atagagcccg attatatcgt caaaaattgc atattgccga tgaatgggga
    20941 actgcagtta ttattcagaa aatggtttta ggaaatatat ctctggaatc cggaaccgga
    21001 gttcttttta cttatgcgga ttttaatgag gaagggggaa tttgtttaaa tggcgatttt
    21061 acgctgtgca gtcagggaga agatgttgta gccggtttag tgcatacttt acctatttcc
    21121 gaaatgcagc gtttacatag caaaacagcg gtttccagtt ccctggaaaa tacctttccc
    21181 gcaatctaca atgctttgat gcgctatgtg aaacagctta ttgaggataa aaattatcct
    21241 catcaggaaa tggaatttac ttttgaaggt tcttctcccg atcagttata tattttacaa
    21301 actcgcgatc aggttattca aaaagctccg gactataagg tcttcgggac aacaaaagga
    21361 gagcaaaaaa aacttgctta cggcattggc atcggaaaag gcgctgtgaa cggaattatt
    21421 gtgttcagca aagaagatat tgaaaaattt gccggtaagg gtgaagccct tattttagtg
    21481 cgtcctgata cggttcctga cgatatggaa cttctttttg catgccaggg attgttaact
    21541 tcaaggggtg gagtaacttc acatgcagcg gttacagcta ctcgtttggg cttaatcgga
    21601 gttgtaaatt gtcgggattt ggtagtttcg gaatcggatt ccaattgcag aataggcagt
    21661 acctatttga aagcgggtga taaaatagct ctggatgcta ccagtggagc tatttattta
    21721 gggcattatc ctctcgttag tgtgcattgt ttataatata ataaaagata gaaggagaaa
    21781 gcaaaatgaa agtgaactta gggaacaaaa aactactcgg aattaacggt ctgggtagaa
    21841 ttggaaaact actgttatgg aaccagttac accttaagta ctttgacgga attgtggtaa
    21901 actgtggaag aattgtaggc aaaaccctgg atgaccttat tcaagtaata gaaatggata
    21961 gcacttacgg ttctatccat aagtttctct atggagttgt aggaaaaaaa gcggaaatta
    22021 aaatcctgga tgctgaagaa ccccttttgg aaattgaagg aatgctggta aaaattttaa
    22081 gaaccgcgcg tgaccctaaa gatattaact ggcttaatga aggggtaaga attgtggttg
    22141 attgcacagg aaattttctg gatcctacaa tgcacactga ttctggaaaa ccctgtttaa
    22201 gaggacattt agatgcggga gccataaaag ttatctgcag cgcacctttt aaaataaaat
    22261 ccggaaatac ggttccggat gatgccaaaa caatgattta cggcattaac catctggaat
    22321 atgatcccct aaaacataat ataatttcag cggcttcctg cactacaacc ggcttggcac
    22381 atatgttgaa accgcttttg gaaaatcaaa aaacttcccg aatattaacc gcttctatga
    22441 gtactattca ttctgctaca aacactcaat ccattttgga tagtgttccg aaaactgatg
    22501 cttccgactt aagaaaatca agaagcgttt taaataatat aattctttcc acaaccggcg
    22561 ttactaaagc actggaacaa gttatgccca atattaaaca tatcggtttt atggctgata
    22621 gcgtccgaat tccaaccacg actgtatctc ttataaactt aaatgttact tttcataccg
    22681 aactggacga gctgggagag ccgttagtaa accgtaaact gatcaatgaa gtgtatcaaa
    22741 aagctgctga gaactcccaa aaagacctgc tgttaatcag ccaccgacaa aatgtttccg
    22801 cagatatgat tggaactatg gctgcagttg ttattgaagc acatgaaaca catacccgca
    22861 caggttttat tactattcct tcatcaaccc tggctgaaca aggtattctt acggataaag
    22921 ttctggaaat gcccgtaact catgctaaaa tctttggctg gtatgataat gaacttggct
    22981 cttatgtcaa ttgcctttcc agattaacca attacataga agaaaaaacg ctgtaaaagc
    23041 taacgagtga ataggttaac gggtgaacgg atgaagaggt aagtaggtta gcaaagaaac
    23101 aggttaacgg gtgattgaga gaagtgaaaa agacaaaaga ggaaacccgc gctaaacctg
    23161 aagaatgtaa tttacccact tagaaggaat attattaccc gaggttgccg aattccgatt
    23221 cggcaacttc ttttttataa attagttatt tgcctttttt atagtgcaga aaagcggaat
    23281 tttccctctc ccaataccct caattttgtt gtttgatttt atcagagcat ttgttccttt
    23341 ctgaaaatat tttttacttg ttccctgcca acaacctatc ctccacccaa cctcaaccca
    23401 tcctaaatgt tgggttgagg ttgggaattg attaaggaac aaataaaaat ttaattttcc
    23461 tttcctttga aaatatctct gtccgattct cactgcttcg cctccgcttt tttcctcttc
    23521 attcttcatt ttgctttcgt catttgttcc cacttgagaa aaaatttact tgcctataaa
    23581 tgcgtatcta taaaaatgcg accaaaaatt gattatcagg acttgaaaaa ttcaagccaa
    23641 agggagtgtt aatgcgcttg aaaacatttc ccggaggtgt tcatcctccg gatgaaaaaa
    23701 ggtatagtgc ctctgtgcct ataactgaag ctccaatacc ccaaaaagta attattcatc
    23761 tttcccagca tattggctca ccttcaaagc caattgtagc tgtgggtgat gaggttttaa
    23821 caggtcaaaa aattgcggaa gcaacaggtt atgtgtcgct tgttcaacat tcttcaattt
    23881 ccggaaaagt tacagccata aaccgttttc ctaatgctac aggttccagt tctatggcaa
    23941 ttcaaattac cggagatggt tccgacaaat ggatagaact tgttgatgaa ccgaatgtta
    24001 tggatttacc cctggaagaa atgaaaaaaa gaattggtga agccggtatt tgcggaatgg
    24061 gtggtgcagg ttttccaact tttgtaaagc tttctcctcc tgcagataaa ccgatagata
    24121 cggttatttt gaacggagta gaatgcgaac catatttaac cagcgaccat cgtttgatgc
    24181 tggaaaaagg tgaagaaatc ttaatcggct taaaactgat aatgaaaatt ttaagtgcta
    24241 aaaaaggaat gatcggaatt gaagccaata aacctgatgc cattgctaaa atgcaagaac
    24301 ttactaaata tgagcctgct atggaagtag ttcccttaaa attgcgttac cctcaaggtg
    24361 ccgaaaaaca gcttatctat gctgctacca aaagaaaagt tcctgctggt ggtcttcctt
    24421 tggaagttgg aattgtagtg caaaatgtag gaactgcctt tgccatttat gaagcgattc
    24481 gttatcaaaa gccgttaatc caaagagtaa taacggtaac cggttctccc gttacaaagc
    24541 cccaaaatct tttagccagg attggaactc ctttttccga actcgttgat ttttgcggag
    24601 gaacaaaaga agaagtagga aaagttattt gtggcggacc aatgatgggt tttgctcttc
    24661 ccactttaga ggcaacagtg ggaaaaggaa gtggtggatt ggttttaatg aacactgaaa
    24721 atgcccgttt aaatgaagaa ggaaattgtt tgcgttgtgc ccgctgtgta gatgtttgcc
    24781 cgctgaattt ggttccctgt atgattgttt ctgccgtaaa atacgataat caaaaacttg
    24841 ctgttagatc agggctgaat gattgtatga aatgtggtgc ctgcgcttat gtatgtcctg
    24901 ctcaaattcg tttggtgcaa tatattgata ccggcaaaat tcgttatgct gaagccaaaa
    24961 agtaggtgaa gaagatgaaa gatagattta ttgtttcccc agctcctcat tttcacgata
    25021 aaaccacaac taaaaatgtg atgtggaatg tggttctggc tttaactccc gctttaattt
    25081 tcgctattta ttattggggt tttaattcgt tgttattaac tctgttaggc gctgtttcag
    25141 cgatggtaac agaggcaata attcaaaaat ttcgcaaagt tccgattacg gtttccgatg
    25201 gcagtgcttt tttaacaggt atgctgcttg cctataatat caatgctgga gctccctggt
    25261 gggttcctat tttgggagct gcctttgcca ttgctattgg caaacaggtt tttggcggtt
    25321 taggcaataa tcctgttaat cctgctcttt tgggaagggc ttttttaatg gcttcctggc
    25381 ccaccataat gacagccggg tgggcaaaac cgaaaagtgg aaccttaagc ggaataaccg
    25441 atccgaatgt gatagctgta aacctgcaaa atttatctcc caaagcttat gatttggtaa
    25501 caggtgctac tcccttaaaa gtagctcaaa ccttaaggga cagcagtttt gtatcttcgc
    25561 ttagtgcaaa tgctggtcaa ggttttgact tggcaaatcg tatttttacc aacttaaccg
    25621 atttgtctgc cttaaaaaat ctattctggg gcaatattgg tggttgcatt ggtgaagtta
    25681 gtgtttttgc tttactttta ggtgcagctt atttgcttta taagaatata attgaatggc
    25741 gaattcccct tttctatatt ggaactgtat ttgtgctctc tttctttttg gctccactga
    25801 aaggttcagg ttttagtccc ttactgccat tcttccatat tttttccggc ggattgatgt
    25861 taggtgcttt ttttatggca acggattata caacaacacc gctaacaaaa agcggaagga
    25921 taatttttgc catcggctgt ggaattttaa ctatggttat tcgtttatgg ggtggttatc
    25981 ctgaaggagt gagctatagc attttgttta tgaatgtgtt gactcctttg attgataaac
    26041 ttactatgcc caaagcattt gggagggtaa aaaaatgaaa atatatttac agcttggctt
    26101 aatattactt gccttttgtg tagctgcaac tgcacttttg gcttatgtga attcccttac
    26161 taaacctcaa attgattcta tacaacaaaa agaagcggaa gaaacccgtc gcaaattgat
    26221 tccggatagc gagtttgagg aaattaaagg ggacttcacc tattacatag ctaaagataa
    26281 taaaactaaa gaagtgaagg gctatgtttt taccgcaaaa aaaaatggct ataacggtcc
    26341 ggtaaaaaca atggctgcaa tagataagga ttttaatatt atagctatta aagtaattca
    26401 acaaacagag acacccggtt tgggaacaaa ttctacccaa ccaaaattta ccgatcaatt
    26461 caaaggtaaa tcggttgaac aattaattgt agataaagat ggtggcgttc ctcctaattg
    26521 tattaaagcc ataacaggag caactatcac tacccgggca gtaaccaatt ccttgaaaga
    26581 atccataaca ttgttaaaaa aactggttct ggaaaaatct gcctctacca tagaaacaca
    26641 ggtaagagaa gaggtgcaca aatgagcttt atgaaagaat taaccaaggg cataataaaa
    26701 gaaaatccta ctttcgtgat tgtattgggg atgtgcccta cattggcaac ttccacttcc
    26761 gtgaataatg ctatcggaat gggattggca gcaacttttg tgttgatttg ttccaatatc
    26821 tttatttcgc tgattaaaaa tataaccccc gataaaatcc gtattcctgt ttatgtaatt
    26881 gtaattgcag ctttcgtttc tattgtggat atgtgtatgg ctgcttattt accggctttg
    26941 cataaatcct tgggtctgtt tattccgttg attgtagtga attgtatcat actgggtaga
    27001 gccgaagctt atgccaataa gaataatgtg atcaattcca ttgctgatgg cattggaatg
    27061 gggctgggtt ttactctggc tctatcggtt gttgctacaa tccgggaaat tttaggtaat
    27121 ggcacctggt tgaatatgag agttatgcct aaaacttatg atccgatgct gattactttg
    27181 cttgctcctg gtgcctttat cactttagga cttttgatag cattgatgaa tatgctgaag
    27241 gagaaaaaat aatgggattc cttgggcaac ttttcacaat ggcaatttct gccattttaa
    27301 tccagaactt tgttctttcc cgtttcttag gcctttgccc ttacctgggg gtttccaaaa
    27361 aaatggattc tgctttcgga atggggatgg ctgttatttt tgtgatgacc ttagcttctg
    27421 ctttttgcta tttgatttat caatacatcc tggttccttt tgaattggaa tatctgcaaa
    27481 ccctggcttt tattttaacg attgcctctc tggtgcagtt tgtagaaatg tttattaaga
    27541 agaattcccc tgccctgtat aaatccctgg gcgtttatct tcccttaatt acaaccaact
    27601 gtgccgtttt aggtgtggct attctgaata ttacaccttt ggcggatgga acgcgttata
    27661 atttcatttg ggctgtctta aacggctttc tttcaggcgt tggtttcact tttgtccttt
    27721 tggcaatggc gggaattaga gaacgactgg aaatggttga aatgccaaaa agtatgcgtg
    27781 gaatgtccag cggtttaatt cttgccggaa caatggcttt ggctttttac ggttttatgg
    27841 gaatgaagtt ttaaaggaaa ggtgaaatat gctttatcta attgttgttc caatggctgc
    27901 aaccggtttt tctgccattt tgttgccggt tgctattatg ggttctctgg gtttgatttt
    27961 tgggctaatt cttgcctttg cttccaaggt ctttcccgtt accgttgacg accgcataga
    28021 aaaaattata aaggttttgc ccggagctaa ttgtggcgct tgtggtcaac ctggatgttc
    28081 aggatatgct acagctattg tgaacgaagg aaaggacttg aatttatgcg ctcccggagg
    28141 tcctgccgta gtagaaaaaa tagctcaaat tatgggtaaa gaagcaactg ctaaagataa
    28201 aaaaattgcc gtaattcatt gcagcagtgg cggtaaaaat aatactaaat ggaaatatgc
    28261 ttatcaggga attgaaagct gcctttccgc agtaaatatt gccgatggac ccaatctttg
    28321 ctcctatggt tgcgttggtt tcaatgattg tgtagctgcc tgtaaatttg atgctatcag
    28381 cattgataat gaaggtatgc ggattattga tagagaaaaa tgcacaggtt gcggcgcttg
    28441 cgtaactgcc tgcccgcgaa aattaataat gctggttccc gcaagtatga atgtgtttat
    28501 ttcctgcagt tccaaagata aaaatccgtt acccaaacaa aattgtggag ccgataaacc
    28561 ctgcattggc tgtggtttgt gtgccaaaaa atgccctgct gaggcaataa ctgtagagaa
    28621 taatatcgcc agaattgatt acagtaaatg taccgattgt ggaacctgtg ctactgtttg
    28681 tccgacaaaa gctattatag acctgaaagc aggttcacga ccggaagctt gatttcctcg
    28741 ttaaccacca caaattcccg aaggttgatt tccttatcaa ccacaggaaa ttacacttcc
    28801 tgcagcttgt cattcctgtg aaagcaggaa tccagaacat aaactacata gtaagattga
    28861 aagtaaaacg acgaggacat cgttcttcct gtaagtaaga aaccattagc accttcagaa
    28921 ttttatccct ttcatattct gaatggtgct tatgatttat aaaggggcaa aaaaggagct
    28981 aaattaccac ctctctatct cattatctca cggataaaag atttgacaaa aaagcccaat
    29041 tgtaaaggtt gactttgaac tttactcttt aagggcaaag ggaaagcaag gtctataggc
    29101 atcctaaact aaaaattatg atggaagtaa aatgcgaact tttgatgctg ttcttcctta
    29161 tttgaagaaa agctacaccc gaattttggg tggactggtg atgttaattc ttgtggatct
    29221 tgtgcagctt gcggttccga aagtaatgca atatgccata gatcatattc aggaaaggac
    29281 tatagatcaa cagggtctgc tttggattgc tttgattatt tttgcctttg ccattgcggt
    29341 gatggtttta aggttttttt ggcgagttct gattattggc aattcacata gtatagaaaa
    29401 aagtttaaga caggattttt acgatcatct gttaacctta tcccagaatt tcttcaataa
    29461 aagcaaaaca ggtgacttga tggcttatgc caccaacgac ctgaatgcag ttcggatgct
    29521 ctttggaatg ggtttaatat cggcaatgga tatagtttta atgactgttg cttcttttgc
    29581 ttttatgatc tctatcaatg gcaagctgac tttgttggca gttattccta tgcctattat
    29641 atcattaact gttatgtatt tcggcaaaaa agtgcatacc cgttttaaag ctgtgcagaa
    29701 aagttttgct actttaagcg gtagagttca ggaaaccatt tccggtatca gaatagtgaa
    29761 agcattctgt caggaaaaaa cggagctaaa aaaaatagat gatgtttctc atgattttgt
    29821 aaatcaaaat attgctcttg ccaagattgc aggtgtgttt catcctttta tgggttttat
    29881 cgtaagcata agtatgatta ttactattta ctttggaggt aaatctgcta ttcggggaga
    29941 aataagtgta ggtggcttca ttgctttctt ccaatatctc ggaatgcttg tttggcctat
    30001 gattgctatt ggttggattg tagatatata tcaaagagga actgcttccc tgaaacggtt
    30061 gaatgaaata tttgaaataa agcccgaaat tgatgatgtc tttgcggatg aacgcataac
    30121 gcatctggaa ggtaaaatcc tatttcagaa tttatctttc cgctatagcg ataatctgcc
    30181 gttaattttt aaagacatca cagcaggtat tgaagtagga aaaacactgg caatcgttgg
    30241 tcctacaggt tgcggtaaaa ctaccttaat tgaactttta gtaaggatct acaatcctcc
    30301 gcgaaatagt atttatgtag atgattatga gctgtatact attccgctga atgttttgcg
    30361 cagagatatt gttcttgttc ctcaggatat tttcctgttt tcagaatcta ttgccgataa
    30421 cattcgtttg ggcaatcctg atgcttccga tgaagaggtt tttgaggcag ctcgtattgc
    30481 ccaggtttat gatgaaataa tggaatttga acagaaattt gaaaccgttg tgggagaaag
    30541 aggtgttact ctttccggag gacaaaaaca acgcgtagct attgccagag cgttgcttac
    30601 taatcctaat atattgattt tggatgatgc tctttcagca gtggatacaa aaaccgagcg
    30661 gcgcattctg aaccgcttaa ttgaaattcg gaaagggaaa acaactctta tcattgccca
    30721 tcgcatttct tccctgcaac attctgattt gataatcgtg attaatgaag gtattattgc
    30781 agaacaggga actcatcaaa accttctggc taaaggtgga ttgtataaag acctctacga
    30841 aaaacaaaga attagagcac gcttggaagg agaagaagaa taatgcatgg tggtggtggc
    30901 ggtggcggtt ctattggacg aggaacctat gctgaagatg aactgaaaac caaaatttac
    30961 aataaacggc ttttcggtaa tatgcttcgt tacttgaaac cctatctcaa gtgggttatt
    31021 ctttctttcc tatttttaat gttgatttct ggtgccgaag ttattattcc tttaattcaa
    31081 agatcggcta ttgatgatca tatcgtttcc gataagtcca ttgccgtttt tgccggggaa
    31141 aatgattatc aaaattttat caaacgctat caaaaactgg gattcaaaga atataaatat
    31201 ggtgaaaatc actttataat tatcaatgct aaagaccgta ataaaataaa tcgtactgat
    31261 ctggagtact tacaagaaaa aggaatccta aaagcagaaa cggtatttct gctaagcgat
    31321 aaggatgaga atattaacat cttaaagaag tatctgcagg aaaatttaca tcctgatacg
    31381 ccaaatcttt ccggatggta taaaatcggc tcaaaaacga ttgcagttcc caaaaaagaa
    31441 ctaaacaaat tacccaagga agagcgtttg aaagtgcgga atgagacagt tcaaaagttg
    31501 ttgattttag ctcttatctt tttggctatt attactttac gctttattgc cagttatttt
    31561 caaactgtct ttactgccta tttttcgcaa aatgcaatgg ctgatttacg acatgatgtt
    31621 tttgcccatc tgcaaaaaat gccaaccaaa ttctttgaca caaatcctgt agggcgttta
    31681 gttaccaggg ttacaaacga tattgctgcc attgatgaaa tgttggcttc aggagttata
    31741 actctaattc aggatgttat tttaattatt acaattgtga tattaatgtt agctttgaac
    31801 tggcgtttgg ctttagtgag ttttagcatc ttacctttag taatttgggt aattgttatt
    31861 ttccggaaaa aaaccagagt tgtctatcgg gaagtgcgaa aacatcttgc agttctaaat
    31921 gctactttgg cagaacatat tgaagggcag aaaattatcc gtctttttaa tcaatacttt
    31981 cataagcgtc aggaatttgc caccatcaat caaaaatatt atcttgcttc cattaagcag
    32041 atacgcttat ttgctttttt ccgtcctatc attcatgttt cttctcagat tgctgttgct
    32101 ttaatcattt ggtatggggg tggaaaaata ctgcaaaatg taattaccat cggtttattg
    32161 atggctttca ctcaatatat aagtaaactt tttgagccca taaacgattt ttcggataaa
    32221 tttaatttct tacagagcgc tttagcaagt gcagaaagaa tattcaattt aatatcgctt
    32281 acccctgatg attatagagt ggaaatgcat aaaaacataa aaatgaaagg cgaaattgaa
    32341 tttcaaaatg tgtggcttgc ctataatgac gaagaatggg ttcttaaaga tatctctttt
    32401 aagattgctc cgggagaaaa aattgccctc gttgggcata caggcagcgg caaaacatct
    32461 attgtaaacc tgattttggg aatgtatcct ttccagaaag gtagaatttt aattgatggt
    32521 aaagatatac ataactattc gttagctgac ttgcgcagaa atgtaggaat tgtgcaacag
    32581 gatgttttcc tcttttccgg aaatataaaa gataatatag ctctcaataa taatgacctt
    32641 accgatgagg aaattatcag agtggcaaaa tatgttaatg ccgataaatt tatcagtcaa
    32701 ttacctcggg gttattacga accggttatg gaaagaggag caactctttc tacaggacaa
    32761 agacagctta ttgcctttgc cagagtgctg gcttacaatc cttccatttt tattctggat
    32821 gaggcaactt ccaatattga tactgaaacg gaacttctta ttcaggatgc tttaaataag
    32881 gttattaagg gcagaacttc tataataatt gctcatcgtc tttctaccat tcaaaatgta
    32941 gaccgcatct tagtgttgca taaaggcgaa attgtagaag aaggttcgca ttttgaactt
    33001 cttgataaac agggcttata ttatgactta tacaggttac agtatacctg aaattgagct
    33061 tgcgcagaaa aagtgattga cagaattgtg ggtttgaaat agtttataat tatggggatt
    33121 gagtttttgt caccagaatg actttttgtg ctccccttat atatatagag tgcttaattg
    33181 cggaagaacg atatcctttt tggttgacga ggatatcaac cttccggtgc aacaataaaa
    33241 agaaaaaaaa cattacttga tctataagga ggatcaatga aaaagtattg gctgattatc
    33301 ttagccctta cactcgtaac agcaatcttt gccgacggca atcgctatga gtttattgct
    33361 tacggtgagg attttgaatc cggggctcct ggctggactc attacgatgc tacggtttca
    33421 cctaataatt ggcatattta caattttggc ggaacccagg gaaatgtctg gtggatggga
    33481 gaccctgctc tggctcaagg aagtaatatt ggtggttatt ataatcatca ataccttgtt
    33541 ttggatactc cggcaagaac tttaacttct gctaatgcca cattaacttt caaaatgcgt
    33601 cataatgtag aaaaccctgc gggagctact gcaccctgga atggttggga tgcctgtaat
    33661 gtaaggattt ccactaatgg cggagctact tggactccaa tatccggaaa tccggcttat
    33721 gattttactt ccgcttattc cttcggtttt gaacatggcg aaggacaggg aattcccggt
    33781 tggggtggag ttcgggaatg gactaattgc acttttgacc ttagtgctta tgtgggacaa
    33841 agtgtgaaaa tccgttttgc ttttgcttcc gatccagctt actgcacaag cgatgcacct
    33901 gcaatgtatg gaatgatggt tgatgatatc agttttggtg gatacagcaa taacggagta
    33961 gatgatggtc aaatgaccta ttcttctctg gttcctgttg gagaagacct ttggcatata
    34021 gcaactgatg ctacagctcc ttcaccttct cattgtatgg tttgtcaaaa tgcttccggc
    34081 agttataatc ctaatatgct gaactattta gtcagccctc ctgttacttt acctaatagt
    34141 ggagatatgt gggtggactt tatgctgaag ggaagttaca atgatccgaa tgccttcccg
    34201 gaagtggatt attttggctg ggaaatttct gtagatggtg gaaatgtctg gtatgcaatg
    34261 agcaatcctt acgggacacc cggaggagat aattatgtgt atacaggcct gcaaaatacc
    34321 tggcaatcta tgaccgaggg atttgggttg agtttcttta ttaccgaata ccacggtgaa
    34381 acagcaattt tccgctggta ttttaagtcc gatggtgata ctcctgatgg agtaggaatg
    34441 tttattgatg acttcagggt ctataatgat gtatttattg ctgctcccga aaatctgaca
    34501 gccgctgttt ccggaaactc agttaccctt acctggaatg aacctggtag tggaggcgga
    34561 ggtggagaag agggctggat ttattactgt ggtgaaccag ccggaaattc tattggtacc
    34621 aatggagctg ccgattttag tgtagctgcc aaatgggatg ccattgggga aacgaatagt
    34681 atctatcctt atgtgggaat gaatattacc aagattaaat tcgttcctgc agaacctaca
    34741 actctatgtt cttatgcagt tcgcatttgg acaggtgcag caggtgtatt agtttatgat
    34801 caggcagttc ctactattac tgaagatgta tggaatgaag tgatcctgac tacaccatat
    34861 actattcccg cagcgaccac aattatggtt ggataccgtt gcaatacaca aggcggctat
    34921 cctgcaggtt gtgataacgg acctcaagtt gagggttatg gcaatatgat ttatatgaac
    34981 aataattggc atacccttac tgctgaaagt gcttcgctta catataactg gactattcag
    35041 gtttatgtag ccgatgccac agggaaagaa tatgtgttgg gtgaattgcc aatgaacaat
    35101 tcctttgcgg aaggtaaatt tggtgttcat tataccgaac ctgtgagagg acgtgatgtt
    35161 accagttata aggtatatcg tgatgccagt tatgtggcta ctgttgatgg taatgtatta
    35221 acttacaccg atactcaagt ccctggtggt cctcattctt attatgtaac tgctatgtat
    35281 ggaactgttg aatccggtgc ttccaacacg gtaagtgttt tcattctgcc cgctgagcat
    35341 tatgaaatca atcacgatga cgggacagca gaacagggat ttagtgtggg tgctactaaa
    35401 cagatggcag ttaaatttga atgcaacgat gaatatgacc ttaaatatgt aaaagtgtat
    35461 gttcatacct taggttctgc cggtcttgtt attcgcgttt atgatgctaa tggaccgaat
    35521 ggaatgcctg gagctcaact tgttacccag tatcaatatc ctgcagcttc agttgttgcc
    35581 ggctggaact ttattacttt gcccacaccg gttcatttga acaaccaagc attctatgtt
    35641 gctttatttg agacatctgg cgcttctcta attggcttgg atacaagttc aaacggcttc
    35701 agttatcaaa atacaagcgg ttcctgggta ccggttacaa ctggtgaaat tatggtgcgg
    35761 gcaataattc agggcggaaa tgcaggagaa gatcttactc ttcctccttt ggttctggat
    35821 gccaggaatt atcctaatcc cttcaatccc gaaacaacta tcagtttcag tattcctgcc
    35881 agcggtcaaa ccagcgtgaa aatctataac ctgaaaggtc aattagtccg aaccctcgtt
    35941 aatgaaacga tgaatgccgg ttatcagaaa gttgtctgga atggcactga tgataatcaa
    36001 aagtccgtag ccagcggtat atatttctat cgcgtaaata atgccggtaa aactattacc
    36061 cgtaaaatgc tgttagccaa gtaggtaaaa gagttattag gttaacaggt taacgagtta
    36121 ataaggaaaa ataatgaagc cccggagaaa ttcggggctt cttatatata attttataaa
    36181 tgacaaacca tctgtcattc ctgagaaaaa aggaatccct tcctttttga aaatgaattc
    36241 cgcaacaagt gcggaatgac agggcaatat tccatctaaa aatgatataa tttaattcca
    36301 atagtgcagg agaaataata aaaagacata tttagcgatc gggttgccga attcagattc
    36361 ggcaaatgct aaaggcacaa atttctttgg aaacctccag tttctttgtt aaagaaagaa
    36421 tttgccaacc cattttccat caactacatt atttgggttt aaggtaaaaa atatttcttt
    36481 attttcgtga taaaaaagag ctaattggtt gcatcatata aggttaacag tccaaattgt
    36541 tcatcttttc ccgaacttaa gttcaaatgc gtaatttatt gaaacccaca agattaagta
    36601 acgactccgt aacacttccg taacacttcc gtaatttcgt tacggagttg ttacggaaat
    36661 gttacttggt cattaagcat tttagcaagc aaaaagaaag agctcgtaat ctttgtaaca
    36721 agcgagaatt gttattgttt atatcgggag ttagcaccga aattgggaat taaaaaaatc
    36781 tttagatttc ttctgcatcc tgaccgtaaa tttctttaaa gcggatatta tcaatttggt
    36841 taggaattcc ttcaaagacc ttttgtcctt ctttcagagc taaaacctta tttccatacc
    36901 tgcgtgccag agttaaaaag tgtaatgaac agataataga aattccttct ctgtttattt
    36961 cacccagata ttgcataatg gaatcggctg tggcagggtc taaactggct accggttcgt
    37021 cggctaaaat taaagaaggt tcttgcatta aggcacgtgc aataccaact ctttgttgtt
    37081 gtcctccgga aaggaatttt acctgtttat cggcaaaatc ctgtaagccc actttttcca
    37141 gattttgttt tgccagttca taatcctctt tgctgaaagg tgaaagagag gaatgatatc
    37201 ctaagcgtcc ggttaaaaca ttggtgagga cggtgaggtt tttcaccagg ttgaattgct
    37261 gaaaaaccat tccgatttgg cggcgataat gtcgcagctc tattccttta agttgcagaa
    37321 tatcttcatt gcgaaaaagg atttgtcccc tggtaggttc aataagacga ttgatacaac
    37381 gcagtaaagt agattttccg ctgccggaaa gtcctaataa aattacaaaa tccccttctt
    37441 gcacttttag agataaatct tccaatgccc attttttacc gtcataactt ttgcttaagt
    37501 tattaacttg aagcaaatta ctgtttggat tcatttaagc aattcagctg ggtccatatt
    37561 cattgtctta agggttgtgc gcacaatttc gtaatcctta tcggaggcat attccaagcc
    37621 atcaatatca taaagagctt tcagaacctt tttcccttcc gcactttggg catagcgata
    37681 taatgcctgc acgataatac cttccagttc gggaggaagg tctttccgaa aagttaccgt
    37741 atcattggga atccaatcag tataatcaac tattttaact ttttcaaaca catcgggata
    37801 ggtctccagc agtttttctc tggcatccat tggttttcct ttggcatcag gtggcgacca
    37861 ataactgcaa cccacatccg ctcttccact ataaaccgct aaaatagctt ggggatgacc
    37921 tcctgcaaag aaataatctt tgggtgtaat acccctttgt tttaagatgg cggaaggata
    37981 aatatagccg gaagtggaag cagcatccgt gtaggcaata atttttcctt caatatcctg
    38041 cagagaatta attttactat cactacgagc tacaaactgt cccctgtatt tggttaagcc
    38101 attgcggaca gtcatcaatc gcacttgcgc atcatatttt tgtttagcta aaatataagc
    38161 ataagttggc aaccaggcaa catccgcctg gtaagttccc agtgcttcaa ttacggcggc
    38221 ataacttgtg ggaacagcta ctttaaaatg taatttggtt tctttttcca gataattggc
    38281 tattgcttct ccgctggaaa caacttttcc tgcttccaga gaaggaacaa aatacatttt
    38341 aatcggattc tgtttagaac caagtttttt attggctttt tccaatcggc tttcttcggt
    38401 tccgcaacca aatattaaaa gtgccacgaa ggcaagccag atatactttt tgcacattgg
    38461 cataaactcc tcaatgaata atttttatct tcttttccta tagagccaaa ctcggtcaag
    38521 aaaaaactac agataatccc agtcctcaaa aaattggtca atcagcaaaa taggattttt
    38581 ccatgtttcc tgaaatgtaa gatggtcttt tccatccaag gtctcgcctt cattgttaaa
    38641 aatgctggca ggcaggataa ttgtttcccc tgcttgtgga gcaagctgtt gccataaatc
    38701 cgaataagtt atcaatccgc aaacacttat atcgggaccg aaaaaatcgt tgtgaataac
    38761 ctgaacgcga ataaactggt tttctaatcg cttattcagg tcttccgcta tgcgggtgat
    38821 aacattttga gcacagacgg aactgatcat taaatagtta accggttttt ttctcagctc
    38881 tttcaacaga ccgcgttttt tttgtttata ggtttgaata gttaaacgca acatcccgat
    38941 tccgttttcc aactggggat aatcggcata aaaatcctca tcgggaatat ctctttccgc
    39001 taaaatgtaa aattcatctg caggataaat aattgtgctg ttatattcct ttcttgcttc
    39061 atcaatcaaa gctaaaattt ctgtagcggt ttgccgatta taaggtataa ttgcatcaag
    39121 gtttttacgg tatttggtta agcccacagg aacgattcca atggaaagac aattaatttc
    39181 tggatcgtga aggtctttca aggtctgttt taatgcctct ccatcattaa tacctgggat
    39241 gcaaacaatt tgaatatgga aacctattcc gtgccgactc aattttttta acacagataa
    39301 ggcatctacc tgttttgagg gacgcattat cttttgtcgc aatgcattat ctgttgtatg
    39361 taaagacaca tacaacgggg taattctctg attgataatt cttgcataat cggtttcatc
    39421 aaggttggtt agagttatgt aattgccgaa aacataagaa tataaatagt cgtcatcttt
    39481 tatgtataag gtgggtctta attcaggcgg catttggtca ataaaacaaa aaatacaggc
    39541 gttttcacaa tagcgaattt tgtatggctc gggttcaatt cccaaaaaac ttttttcttc
    39601 tctttgaatt ttaactttac gagagatacc ttctgcagac ataatttcca gctcaaattc
    39661 atagtccgaa gtataaaatt ccaaatccaa aaagtcattt actgcctgtc catctacgga
    39721 aatcagtttt tctccgcttt taatgtcatt tttggctgcc aaacttcggg acagaacatt
    39781 ttgaattaga atagccattg tttttctctg ttttcttttt caggtgtttc ttatcctgtc
    39841 gtaacggaac ttaaaacgct tgataccttc aaaaatagtg cgtgccagac gatcctgata
    39901 ttcttcattc accagtaatt gttcttcttc aggatggctg ataaagccca tttccactaa
    39961 aatagaaggc ataaaagcac ctcgcaacac ataaaaattt gcctgtttca cgcctctatc
    40021 aatactttgc gtgccggcaa tcagattttt ttgcacattg aatgccatat tgttactgtt
    40081 ttccaaatgt tctgtctgag caaggtcgct gaggataaag gaaaggtcat cgtatttctt
    40141 ttttgcttcg cttcctcctt caaagcgttc tacaacagca ttttccatag cttccacggc
    40201 tctggcatcg gaggtttgag cagtggataa ataatatgtt tcaattcccc gggaagttgt
    40261 tgacttactg gaattggaat gcaaactgat aaagaggtca gctctttttt cattggcaaa
    40321 acgggttcta tcatataaag aaacgaaacg atcatcttcc ctggtaagta aaacattaac
    40381 ccccagttct ttttccagca agttcttaag tttcaaagca acagcaagat taatatcttt
    40441 ctcattggct ttcagttttt tccctatggc tccagggtct ttaccgccat gccctggatc
    40501 aagaacaatg gtctttacgc tattatcaat tggttttgcg atttgtaaag tgcttccttt
    40561 gcgttgaatg agagagggga aatgtgtctt aagttgttca gttacaaaaa cagaaggcag
    40621 atacaaactc tctcctttcc gtaaaagagg ataatgcata ttgtaagaaa cagttttcag
    40681 agtataataa ggtgaatttt ccaggaagat aaactgttca tcatagaggt tcaaatacaa
    40741 tctattgtca cttctatcct ctttactgat ggctttaaaa atagcgctga aatcctttaa
    40801 agctacataa gaaacagaag aaagcgttgt ttcttgcaaa ttaacaggtt ttgcttcgcc
    40861 cttaatttgg atagtgatat tacccaaaag cattcctaaa caggtaagca gggcaaaaaa
    40921 gacaaagaat ttctttttca tagtgtaagc tccaattggg gattaaattc cgaagcggga
    40981 tttatttcct ccggtgcagg agaaaaattg ggagtccaag tatccgaact ggaaatttct
    41041 tgagtgtata atttggtaaa accaagttct acagctgtat caactacctc aaagtattcg
    41101 gataaagtaa ttcctcttgt tagttcttta tagttttttg cttttccggc aggataatat
    41161 tgtcccataa gagaaagagt tagctccgtt cccaattctt ctgttagggt gtataaattt
    41221 tctttgcaac cggctaattt attgggtaaa acaagcattc tgagcagcaa tcccttttga
    41281 gcaattccct tctcgtcaag ttttaagagc tctacttgag aaaacatttc tttgatggca
    41341 gaaatggcta cggcaggata atctggagca gcagaatatt tttgggcata aaccttatgt
    41401 gcatacttga aatcaggcag ataaatatct accagtccgg ttaaaagttg cagtgtttct
    41461 accttttcat aagcggaaga attccacagg acaggaatag ttaaaccctt ttctttggct
    41521 tgaataatgg cttccctaag ctgaagagta aaatgtgtag gagttaccag atttatgtta
    41581 tgagctcctt cttcctgcaa ctttaacatc agacggataa gctcttcaac tgatatatct
    41641 tttccccaac ataaagtaga aatctcataa ttttgacagt atacacagcg taaattgcat
    41701 cccgcaaaga aaatagtgcc acttccgcgt gttccgctaa aacagggctc ttctccgaaa
    41761 tgaagtgtag ccaaatttat tctaagctta tccgtaacac cacaataacc tttgttcttt
    41821 gttctatcaa ttccacaatt gcgtggacaa atattacagg aaaacatttg ggaataggac
    41881 atttaatcct tcttcaaagt aaaagcggag ggaaataatt ctgttattaa agctttgtga
    41941 attgccttcc ggttagcata tattatttca atctccggtg caaattcata aattacttgt
    42001 ctgcaagcac cacagggagg aaaactttct tcgctatcca cataaacagc catttccgaa
    42061 aaagttctat gtccttcact gattgcttta aaaatggcat tgcgttctgc acagatagtt
    42121 aaagaataag aggcattttc tacattgcaa ccggtgaaaa tatgtccgtc tttagttttt
    42181 acggcacaac cgactttgta atgagaatag ggagaatagg agttttctgc tgcctttttg
    42241 gctgcctcta ataattcttt ttcactgaga tttaattcta ccatctctat cctcttttta
    42301 agccaatagc gctaaaatta agccaccagc aataacagaa ccgatttgac cacctacatt
    42361 tacactcact gcaggcatca ataaaaagtt gaaagggtct tcctttagcc ccatttgatg
    42421 aataactctg gcactcatag gaaaagcaga aattccgcaa gcaccaatca tcgggtttat
    42481 cttttccttt ttaaatacat ttagtaattt ggcaaaaagc actcctccgg cagtatcaaa
    42541 aataaaggca acaagcccta aagctaatat taacaaggtc tgcggaacca gaaaaagttc
    42601 accttgcatc ttggaagcaa tagttaaacc caaaagaatg gttactaaat tccccagttc
    42661 attttgagcg gttttgctaa gcccttctaa aacaccggat tcccgaatta aattgccgaa
    42721 cattaaaaaa ccaattagcg ctaaagcagc aggaacaaat attccggcaa taatggtaac
    42781 agcaatagga aacatgattt taacaatttg cggaacatca ccactgtgat aatccatccg
    42841 aattaggcgt tcctttttgg ttgtaagtaa acgaattaca gggggttgaa taatcggcac
    42901 taaagccata taagaatagg cagcaacaga aattgctcct aaaagattgg gagcaaaacg
    42961 attggctaca taaatggaag taggtccatc tgctgctcca ataattccaa tactggctgc
    43021 ttccttaatt tcaaaaccta aaccggcagc taaaacgata gttacaaaaa tacccaattg
    43081 agctgctgcc ccaaaaagca gcataaaagg atttttcagt aaaggagtaa aatcaatcat
    43141 tgctccgatg gcaataaaaa taagtagggg aaataactct gtatcaattc cggctttaaa
    43201 gagaatacct aaaggcgaat gctctcccac agcagcagaa aacggtatat tggctaaaat
    43261 agttccaaaa ccaataggta acaacaaggt tggttcatat ttatgtttta ttgccagcca
    43321 aatcaatatc agtccaataa taatcattat aacctgtttc agttggaagc caagcaagcc
    43381 ggaaaagagt gtttccatta gcaaaactcc tatgtcttat tgcatttgcc actatcctga
    43441 agagggattg tttgtcaagc aataacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43561 nnnnnnagtt atctgcgttc tattttttct accctctcta cctcaagacc tcaagaccca
    43621 aattttggtt gacacaatct taaacatctt ttttatgaat ttatgagaaa tatcgtttta
    43681 attctgctgg ttgctctttt actgacaggt tgtgatctgt tttcagttcg tgattcagaa
    43741 ccacctgcag cacagatacc gtggatagat tttaccacag aggttgattc tgtgttgtta
    43801 aatctggaat acagttatga atatcctcaa aatgcagtta attatgcacg gcttttcagt
    43861 tccgattatg tatttcattt tgccccgcag gatgtgagcg atttttctat agagcaaacc
    43921 tggacaagag ttcaggagca ggatatgatt caggttttgc ataaccgcta taaaaatatg
    43981 caggtaacct tagagaaagt ggatgatcag gatcagatag aatcggcgca agcaaaaata
    44041 taccgtcaat ataatattac aggtatttcc cgcaatgagg gaaaggaagt tttaactttg
    44101 gcggaaggga atatggaact gcatttaaaa aaggaatatg gctactggta tataacaaag
    44161 tggtatgatt accgcagtag taatgttcct acctggggaa agttgaaata tgagaaccat
    44221 tagcattatt ctgctttttt tgactcttct gattggcagt tgcaacaatc ctttctgtcc
    44281 tgaactctat gatgtgtcca atgaggaaat tcaaaatcgt actcctgaag aacttttaca
    44341 aaatttggaa cgagcataca ccgaaaggaa tatcaatatt tataaatccc ttttgcatcc
    44401 ggattttcgt ttcgagcttc tggcaagcga agtaagttta attggaattg atatgaataa
    44461 tgacggaatg cgtgattcct ggtggggtta tgcacaagaa gtggaatata cggatagaat
    44521 gtttaataag ggttccagtg atggaactta tcctcctccg gatgatatta agttacggtt
    44581 acaaattccc ccttctgaaa gatgggagaa tgatccggaa gtaggtcatg aggattggat
    44641 aataattcct tgtaattttg atttaatcct ttcctatcag gaaagcaatt cttctattac
    44701 tgccaatggc atagcccgat tttacttgag gcctgtagga aacggttgga aaattgccat
    44761 ttggagagat gaatccaata tatgagttct aagggacgca ttcttgccct ggactatgga
    44821 gaaaagcgta tcggtattgc cttaagtgat ccgaatcgta ttttttccaa gccgctgtgt
    44881 gttctttcca ataaaggttt tgaacaatta gtggaggaat tgaaaaaact aattgctttg
    44941 catcaggtca ctttgttaat aattggaatt ccttatgcca tagatggttc catcactccc
    45001 aaaacggaag agtgtcagca gattatgaat cgcttaaaag atgtcttatc tattccggtt
    45061 gagggttttg atgaacgcta ttcaacctgg gaagcaaatg aagaactgaa aaaaatggga
    45121 tataactgga gacaagcaaa agaaattaaa gatgctatgt ctgccgcaat tattttaaaa
    45181 gagtttctga accaatgaaa aaagcaaaac catttcttat cggtttggca atcttattgt
    45241 gtctgttaat tttggttatt gtttatctgg tttttgttcc tttgcatagt gaagagcgca
    45301 ttgtgcgaat taaacaagga gataatgcca gaataattgc tgctaaactt tccgaagcag
    45361 gaattattcg cagtaaaaca atgtttatta tcctcaccaa aatcagaaaa gcagatagaa
    45421 acctgaaacc aggaagttat atttttggag gcaataccta tctttggcaa acggttagcc
    45481 gtttatataa aggtcaaaac gaaagcataa caatcacttt tccggaaggt ctttcgctat
    45541 ataaaaccct aaagaaaatt gatgccagtg gcttggcaac ttatgaggaa ctgcataaag
    45601 cagcaacaaa tcctcttctg gtaaaaaaac ttaccggttt tgatgcccta tctttggagg
    45661 gttttcttta tccggagacc tatcgctttc ctattgaaat atcgccggat tcaattctgg
    45721 caattcccgc aggtgaattt ttccgccgtt tacagaagga agggattgat ccttttgctg
    45781 ttcctgattt ttatgataaa ttgatccttg cttccattgt ggagaaagag gcaggggatg
    45841 aaagcgaaaa agaaattatt gccggacttt ttttaaaccg tatgagacag caaatggcac
    45901 ttcaatcctg tccaacaatt gactatattt tggagcccaa aggaattaaa cgcagtgtgt
    45961 taacttatgc agatacccaa atcaattctc cttataatac atatctttat caaggattgc
    46021 ccccaacacc aatttgcaat ccttcaatta caaccataaa agcggttttg aatgctaaac
    46081 ctaattccta tttatatttc ttttctgatc gccaaggaaa aaatgtgttt tccaaaactt
    46141 atgaagaaca tttggcaaag cagagaacga tgctgtagag tttatgaggt caaaagtttg
    46201 aaggtcaaga ggttagtaga aaggtaaacc cttaacgagc ataggaaaaa tggtgtagcc
    46261 ggaggaagca gttcctccgc aaagaatgaa tgctgaacta ggaaaaagca taattcagag
    46321 taaaaaaacc gcatattgga taatttgttt catatattta agatgaatgg ctgaaattat
    46381 gaaaaccaac acgaacaaac agaacttgac aagatatggg tttatagcta tattatataa
    46441 gggagatata atttaatgct tggaccttat gaaataaatg atatatacaa aaccgactgt
    46501 gttgaaggtt taaaaaaact gccggagaat tgtatagacc tttgtgtttc atcacctccc
    46561 tatgatggaa taagagatta tcatggtttt tccttagacc ttcataatgt aggtatagaa
    46621 ctttaccggg tgatgaaaga aggtggtatt gcagttatgg ttatgcaaga tcaaaccagg
    46681 aattttgcca aatcattaac tactttcaga actgcaattg actggtgtga taatgccgga
    46741 tttcgccttt ttgagacact tatctataga aaatacggag cggaaggtgg ttggtggaaa
    46801 accagattta gagttgatca tgagtttatg ttggtttttc ttaaagggca acgacctcag
    46861 tattttgata aagaaccatt aaaaatccct tcaaagcatg gcgggaaaac ccttacaggc
    46921 ggtggaactc gtttaacaaa tgggattaga attccaacca gacctattct aataaacgaa
    46981 atgaaatgcc gaggaacggt ttgggaatac cttacggcag gtgacggttc acgcttgaaa
    47041 cataaacatc ctgctacttt tccagataag ttaccttatg attttattca gtgtttttgt
    47101 ccaccagaag gtattgtttt agatattttt atgggttcag gaactacagc tcttgccgct
    47161 attgaactaa accgtaattt tttgggtttt gaaatatccg aagagtatgt taaacttgct
    47221 aaacagcgga ttgccattga aagcaagaaa gacgaagatc agcttatgtt tctgtaactt
    47281 tctattgtct cttggttaat tatatgttct taaaaatgtt gtgagggtct atagtatatt
    47341 ctattaaatt accgcttgtt tgtgccatag ataatggatt ttcggaaaga atttgtctaa
    47401 agattcctac ccgcgcttta ttacttttct tcgtaaattg aaagacatta aaatccgatt
    47461 taatccattt gtcttgaata tagttccatt ttgtctttgc tgataagtat ttcaggaaat
    47521 tttccgtttt catccaggta ttatcatcaa agcggaaaaa agaattgtct ttttggagac
    47581 catcaatctt tccctcacga gtagagattt taatcttatc actatagtca tcctggctta
    47641 taactttaaa gagttctttc aatgctatca ttaaggatgt gattgtgtaa cattcattaa
    47701 agaagatttt atgcagagca gatgatttcc tgtcagctcc taaataatgt aaccagctta
    47761 aacagttctc gggatagttg aataagttct taaaagtacc atctgtaaaa aggagaggac
    47821 agatgctttc tgtttcaata ctaatttttt cctgattgtc caatattcca attagtaaat
    47881 ggatcattgc taaaggatat ttgtttttaa aatgggtttg accataatag ccccgcagaa
    47941 gatttggctg aatacattga tttaagttag tccataattc aggaattgcc ctttgggaat
    48001 ataaaactat atggtgaccc gcgcttctga taaagtccct aaatatttcc atatccaaaa
    48061 aaatacattc attaagatca attttcttat cttttatcag gattttacca tcataaaagc
    48121 caactatttt ccaatttaga taattagaca taaacaaacc acataataat ccatccgaat
    48181 cagggcttaa aatgcagtta tgatctcttt ccaaaatcca gctatatttc ttaattagtt
    48241 cttgataatt gaggacatct aaattatttt ccaaagctga ctccttgtaa attttaatct
    48301 ccttaacttt aaatttgttt tgccattatc tgtcaataat tatagtcata acccaatcaa
    48361 tcctgtaatt tggaaaattc tgaaatcctt atttttttgt gtttggtatt ttcaacttga
    48421 catatacctg ctgattgatt aaattgtata tttgactata gagtatgaaa agcaaaaaga
    48481 atatttaacc ctaaagggag gaaaaatgaa gttacacaag atgatgttac tcttagtttt
    48541 agtggctgta attgtgatga ttgcatcttg cagttcaaac aaaaaagcca atattaaacc
    48601 acaaggtgag gaagtttacc gtccagcttg gtgggctacc caacctgaac aggattatgt
    48661 ttgtacttat ggacaagcaa ccaaggtttc ggaaaattta tcagtggaag cagccagggc
    48721 aaatgctctt caggaagcag cacagtatgt agaggttttg gtgaatagta tgctgaaaaa
    48781 ttatgaagaa gaagcaggtg ttaaagaccc tcaggtttta gctcttacac aaaatgtggt
    48841 aaaagccgtc tcttcggcaa aatttaccgg tgttctaatg ggtaaaattg agaccagaaa
    48901 ggttaatgaa ccagatggtg ttcgctataa aacctggatt cagttgaaaa tacctaaaga
    48961 tgaaatcaac agaaaattgg cggacagcat tcgcaacgaa gaagcacttt acaatcagtt
    49021 taaggcatct caggcatttc aggagctgga taaggcagta gaaaaatact aataaataaa
    49081 taatgtgtag ccggagaacg caatttcttc gcaagaaaaa aaatgcagag cttcagcgag
    49141 ctccgacttc attccaagtg aaaatttata acgaaaaaag agagctacaa aagagaaaaa
    49201 ttccgtagga acgatagata ttagagaagg gtttcaaccc ggagaagaaa agacaaattt
    49261 tgtatgtggt ttgctgagtt ttaatttacc ataaagagag gaaatttatt tctcggagtt
    49321 ctgcaaactc tttggcatat atatgaactt accaaactat tttttgctca actatattat
    49381 ttgggataaa ctataacaat ctgtgaaaga tttgcactat ctaagttatt gtacccagca
    49441 agatacatag tgactactca tcctctaccc aactttgagg atgggttgat gttgacaagc
    49501 aagcaaggaa aaaacaaaat cccttgacga aacaagcagt tccaaaagta atgcaacgac
    49561 tttacttttc aatatattgg agcgataaat gctaaaaata ggtcttaatc aggaaatggt
    49621 atccagagcc cggaaagcgg catttcagat aacaaatgct tttacttggc tgtttgaggg
    49681 ttatactact gtagccacag aaaggacagt attacgattg atgggaatag atggtgccct
    49741 caaagatggt aaacccttgc ccaatgttgt ggtagatcat ttggtggaaa attctgctct
    49801 tcagaaagga gcagcaatct gggtggctaa tgctatgtta aaaaagggtt taacagcaca
    49861 gcaagtagcg gaagaagttg cctataaagg tttagatata acccaaattc ctcttgcctc
    49921 aggcgaagca atttgggaaa ctcttaatcc tttggtagat aaaatgttgg gtcatataac
    49981 agcccaaaaa caaaagcgag aggaacttct ggctaaatat cagccccgtc cccaaccagt
    50041 tatctatgta attgtcgcta ctggaaacat ttatgaagat gtagtccaag ccaaagctgc
    50101 ggcaagagaa ggtgccgatg ctattgcagt tatccgttca acagctcaat ccttattgga
    50161 ttatgtacct tttggtgcca ctacggttgg ttttggaggg acttttgcca cgcaggaaaa
    50221 ttttaaaata atgcgtaaag cgttggatga ggtagctgaa gaagagaacc gttatattta
    50281 tttaacaaac tatgccagtg gactttgtat gcctgaaatc gcggctatgg cagcaatgga
    50341 acgcttggat atgatgctta atgatgcaat gtatggcata ttgttcagag atattaaccc
    50401 caaaagaacc ttgctggatc aatattttgc gcgaatgatc aatgcctatg cgggaattga
    50461 aattcagacc ggtgaagata attatttaac tacttccgat gctgtag
//
LOCUS       DAOOZV010000006        50279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_797_length_50279_cov_60.443871, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000006 DAOOZV010000000
VERSION     DAOOZV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 50279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50279
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_50279_cov_60.443871"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(486..1160)
                     /locus_tag="PLW08_01310"
     CDS             complement(486..1160)
                     /locus_tag="PLW08_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPL51278.1"
                     /translation="MSLFSAIIRLSVFSLNKNKFSSIIVLPDCVIKYYSSYKHFQAEL
                     FAYQLNLPMLPKLLDYAEPYWIKMEKITGIPYLDELNNFQPALLAETIANFHWATLQN
                     DKCLCHKDNAPKNILFCQGKYYFIDFTESEFAYPEKDITHLLLFWAADFPGEFFRSSV
                     TLFLNIYLSILQLNSERWMTSLQENICLFDNRRKNFGKHSGKQPIAIQSANRKWLAEL
                     EELINS"
     gene            complement(1111..1926)
                     /locus_tag="PLW08_01315"
     CDS             complement(1111..1926)
                     /locus_tag="PLW08_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPL51279.1"
                     /translation="MKAKTLYLILILLVLVAAIITLILLSRKDPLNGPESIAFDAVGN
                     RFLISNTLGKSIVALSLDEQYSPFLDEGLEEPRGIIIRAGKLYVADKNAIKIVDPEKA
                     VIIDTLKIPGAVMLNDLAFTKTGIIYTTDTGGDCVYVIDPIANKMQKLTSPLLKKPNG
                     IVYDMPRDQMFVVCFKEKSPILSVSTLDNRISIFKDTIYDYLDGIAIDDLGRIYITSW
                     GQDMIFEIPQEQNRYLPTFKGIKDAADIFYYLPANQLIVPLFSDNQIIRLQLE"
     gene            complement(1923..3332)
                     /gene="cysS"
                     /locus_tag="PLW08_01320"
     CDS             complement(1923..3332)
                     /gene="cysS"
                     /locus_tag="PLW08_01320"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPL51280.1"
                     /translation="MLLYNTQTRKKEEFQPITPGKVGIYACGPTVYNYFHIGNARAFI
                     FFDVVRRYFAYRGYEVTYVQNITDIDDKIIAQANEENISFTLVAEKYTRAFLEDTSAL
                     GIKPPTYQPKATEVMDDIIAFIAELEKKGYAYEVEGDVYFATENLPSYGCLSGKKTEE
                     QLAGARVMENYNKRHPADFTLWKKGKPGEPVWESPWGKGRPGWHTECVVMSQKYLGET
                     FDIHCGGIDLIFPHHENELAQAMAYSGKPLANYWMHNGFLNVDGEKMSKSLKNFFTAR
                     DILAEYDAESIRFFFLSKHYRSPIDFSRELMEESQRAVANLYSSLAEINYPENAENRR
                     PTVCGTAQELQNLEQEFILAMDDDFNTAKAISLLFELNRKVKNTSATMADRISAAIKM
                     VELGSVLGFFQNLEAKLKQKMPDLSKDLICLILSYRKEARDKKDWALADKIRNDLAQL
                     GIEIKDTPEGVKWTLKEKK"
     gene            complement(3343..7179)
                     /gene="porU"
                     /locus_tag="PLW08_01325"
     CDS             complement(3343..7179)
                     /gene="porU"
                     /locus_tag="PLW08_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPL51281.1"
                     /translation="MKRFFLAVILVCSLAFLNAKITLIRSSETELLVEYTIESFKITA
                     EGEFSRIIAENMGHNSIPGTPSIPYTEFKVGIPPNGSINCSVLNSNTTTIILESKLIP
                     VPEIKSGEEVDYYLYNISPEAYQPQRGEFIIQLPDTNFRGYHFVPVTLHPFVYDGNKN
                     LTITTKATIKINISGQTNFRSLEESDKGADLLLAQLINSQQAKNWRSNPRPVINYAPF
                     GLSNWWMRIETNRPGIYRINYSDLSSLPLNDIDPTTIRIFSTSGKVLGNNINNNGAEF
                     REIPIYISGEADHSFNEQDYILFYGSFRDGYEQNAEVQSDPLYYNPYSQNQVFWLTFG
                     GSFSGNPLRIQTVSSPSTYDFSLTSTPVIKQIETETHRREKYGFTWFSETFFGNNTTD
                     YILTTELSDVSGSDDQKIQLRLCQESVGEGTVHKIQISVNGMPVYNNQDLHTLDFTWT
                     GTNYFYIDRPISNLINGINTLKIRVFRNSKDNLYFDYYRFSYQQNLNKGNAQKQFFHK
                     NSMLPTICKFNLSGDLSNTLVFRINNLYSVDLIPVADNCIIADGNSKTFYFMLKPGEA
                     YRPSVMQLVQPNDLTYESDQFDNIIVTPAEFLQQAQELANKYREIYQISSKVILVDDI
                     YNQFNGGHIDPAAIRLALKYYYFNLPAPRITSLTLLGIGTMDWRNFSGFSAPKNKMIV
                     WQGSYIVSDDYFGMFNTSYYPELAIGRYPVKNTNELNIMLQNFTNYTLNPTPGWWRNS
                     MVFIADDLTNGDTNYEYILTQFAESNSNDVHPSNLVDKIFAMEYEYDEFQNKPKARDD
                     LFKAINEGRLLMHYTGHGSYDKLGAEDYMNGATDMGRFANEGKLTFFIAAACEVSQFD
                     YWGFDCLAQKVVLLNNLGAIASLSATRKSFADNSSILLKYVLESLAYNRNPLGYCIMD
                     GKFRYTGGATNNEMYILFGDPVIRVVPPERDSTMLVVTSTDEDILHSRETAVINGSFA
                     NSNLSGITEIKTFDSKRAYSLGPATNVTKMGNQFYRGSSEVTSSHYNSSFIVPDDIVS
                     GSTGLIVAYLWDETGKKDYSNYYYPLQLSDEAVDVANPDVPQINIYLGSKDFRPGDIV
                     GTNTTLYADISDSNGINITGSSGHNILLVIDNSLQPIAITEYFNYDKNSCTSGTLIYP
                     LKDLSEGMHTIQIIAFDNFNMPAVATTTFVAKKSSELAIENLLIYPNPISKDGYISFM
                     LSMDAEVNIGIYTIRGKRIANIKIYGKQGFNKVYFNGRDDKGDTLANNTYFVKVQARS
                     ANGKKVEKTEKMVIYK"
     gene            complement(7176..7328)
                     /locus_tag="PLW08_01330"
     CDS             complement(7176..7328)
                     /locus_tag="PLW08_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51282.1"
                     /translation="MLLLLVSTIENITLALRILIQGMLGIFIFMAVFYLLIYALEHLF
                     RPKEKP"
     gene            complement(7341..7805)
                     /locus_tag="PLW08_01335"
     CDS             complement(7341..7805)
                     /locus_tag="PLW08_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPL51283.1"
                     /translation="MKLTLLQIGKTKDKWLLEGISEYRKRISAFAKFEIIELPDESIK
                     HSGNAESVKAKEASTILKYIKPEDYVILLDEQGEQKNSLEFADFLISLSERKNVVFIT
                     GGVFGVDKSIRQRANSILSLSKFTFTHRLVRLILCEQIYRALTIINNRNYHK"
     gene            complement(7814..8713)
                     /locus_tag="PLW08_01340"
     CDS             complement(7814..8713)
                     /locus_tag="PLW08_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPL51284.1"
                     /translation="MFKNFAELLSYVKQNRKGTAIIAGAQIESAIEAGVMAKKEGLCE
                     SILVGDKPEIIRLIQQKAPDLVNAFEIIDTGSDLRKAAETAVALAKEEKGDLILKGKM
                     ESSVILKAALDKEKGLKVSEVISDVFVYEHPEGLKIQTDGGINILPELNEKIAIVKNA
                     VQVAHSLGNPKPKVAMICAIETVNPKMPATIDAALIAKMNERGQIPGCIIEGPLAFDN
                     AVDKEAARIKGLTSEVAGAADILVFPNIETGNVHGKMLTYYCHYKIAHVTIGTKVPIL
                     IPSRADSGETKMLCMALALATMS"
     gene            complement(8788..9666)
                     /locus_tag="PLW08_01345"
     CDS             complement(8788..9666)
                     /locus_tag="PLW08_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPL51285.1"
                     /translation="MSNKESVFADKALQNYLNEISKFKTLSREEEHDLAIKAKNGDVD
                     AMNKLIQANLKFVVKIASRYQNRGLSLSELISEGNIGLIKAIEKFDPDKDIKLISYAI
                     WWIKQRIMLAVSEKSSLIRVPMGKSSAAHRVKLTQDRYFTETGKIATPDELSEKMNIA
                     EKTIAQLQNKLPETSSFDDIITTSDSQDFTAKDLLEDKDTLDPQSLYYQERMQEKIQN
                     AISKLDAREADIIRNYFGLNESHESRNFAQIAEKLGLSRERVRQIQKEALKKIMREIK
                     PEEDEFVDRFLEKYHY"
     gene            complement(9677..10885)
                     /locus_tag="PLW08_01350"
     CDS             complement(9677..10885)
                     /locus_tag="PLW08_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPL51286.1"
                     /translation="MTLKLDRLLWLVIMQIMLILPLGLSAEDQIHIIKKGDTLYSLSK
                     RYGTTVDELKRLNNLSDNNLSIGQKLIVKKDTEPIKPVAPVPVTKPVTPEISGGTSSP
                     QISSLPGAETNPSLSPAKKIPADYYYTVKTGDNLYRIAVNHNITLGELLAWNNLANSS
                     IPIHPGDKLVIKNPAELPETKIVPEEIITESNPVISTVPAKKDTVLIERVYVVQKKDT
                     LYRIATNNGMTVEELMQLNNLTSPDIKVGQKLYLSGKPRPEITTPPEVLTEEEIMKRE
                     KIRTDLIMPVEGKVISEYGLRNGRPHKGIDLGAKAGTPIYAVLDGTIVYSGVQGSYGN
                     VIVIEHPDFVMTVYAHNEKNLVNVNDVVKQGQQIGTVGSTGNAQGTHLHFEYRLKGKA
                     INPRKVLPLP"
     gene            complement(10866..11135)
                     /locus_tag="PLW08_01355"
     CDS             complement(10866..11135)
                     /locus_tag="PLW08_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPL51287.1"
                     /translation="MPTWELYANGRVQGVGFRYYVWKIASELGIKGYVQNQWDGTVKI
                     VAQAEEHTLQTFQQIVSQGTKYAQVKELQIYKLDSVEKYNDFEIR"
     gene            11316..13034
                     /locus_tag="PLW08_01360"
     CDS             11316..13034
                     /locus_tag="PLW08_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPL51288.1"
                     /translation="MNLLHIINLVGGLALFLFGINKMSDSLQAVAGSEMRRILKVLIN
                     TPLKGVLVGLGVTVLIQSSSATTVMMIGLVNTGIMTLNQAVGVVMGANIGTTITAQLI
                     AFNIGQYAFLFVILGVTLLFIRKSKTMEHWSQILIGFGLLFVGLNVMGNSVSPLQNSI
                     TARNLMIRLASNPILSVLVGTCFTMLIQSSSASIGIVMVLASTGLIPFEGALYLVFGD
                     NIGTTITAWLAASSSGNTAKRVAMVHTLFNVFGTIIFGILTYLGIYTLLINWITPGNV
                     YAGQNIARHVANGHTMFNVINMILFLPFANQLANLATKIIPPDKVETISLGEPKHLNY
                     TIISNSDLAINQSIKEMREMLRLVRMELMLAYQAFKEKDYKKQMRVSRIEKAIDNLQR
                     EITLYLVAVNEKTNAEDIIHKIPALLHTVNDIEKIGDFTEEINKILNEQIIAKKHHLS
                     PKFISIIDELHSKLLYMLDLSIDYLVELKEDYIYKIIEMEGRINETHHHLREEILSQV
                     QNGKCNAAEGLNTIDYIDEVEEIADKLKNLVKAGTHNFVYNRNFVKETEEEEKTENDK
                     ELTDVF"
     gene            complement(13031..13999)
                     /locus_tag="PLW08_01365"
     CDS             complement(13031..13999)
                     /locus_tag="PLW08_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPL51289.1"
                     /translation="MNIYETVQEELAKEATAIQKVAKQLSKESLEKAFDLLCKCKGKV
                     VLTGMGKTGIIARKISATLASTGTTSIFLHAAEGIHGDLGMIESGDVVIAVSNSGNTQ
                     ELINLIPFLKFKYVPIIAITGEPNSQLAKNSDVVLNCHIPKELEPLGLVPTASTTVAL
                     AVGDALAIALLKHKNFQLKDLAKFHPGGTIGKKLLLRVSDLMHSGKELPVIEEKAKMS
                     EAIMEMTSKKLGCTAVTNKDGKLTGMITDGDLRRQLHNKGNSLLSYTAKDCMTANPKT
                     LKPEVLAIEALNLMETYKITMIPVVDENNVPIGMLHMHDLITAGVI"
     gene            complement(13996..15897)
                     /gene="thrS"
                     /locus_tag="PLW08_01370"
     CDS             complement(13996..15897)
                     /gene="thrS"
                     /locus_tag="PLW08_01370"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPL51290.1"
                     /translation="MIKITLPDGSIREYSAPVTALKIAEDIGPKLAEEAICAEVNNKM
                     VDINYPIDTDATVVIHTFNTEKGKEVYWHSTAHLMAQAVKQLFPEVKIAIGPAIEQGF
                     YYDFDRDESFTEEDLQKIEAKMQELSTQALPYYRTELSKAEAIKLFSEMNETYKLELL
                     EDIPEGEIISSYRQGDFIDLCRGPHLPDTGKIKAIKLLRTSGAYWRGDEKNKMLKRIY
                     GISFPTEKELTNYLHFLEEASKRDHRKLGKDLDLFSISDEVGPGLVLWHPKGAMVRHL
                     IEAYWKEQHLKKGYQLVYTPHLGRANLWETSGHLDFYKENMYSKMDIEGQDYYIKPMN
                     CPFHISIYNSSLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTPE
                     QMDMEVDKLIVFSLDMLKAFGFKEFLIYLATKPDDCVGEKEDWDKATASLANSLQKLG
                     LEYKVDEGGGAFYGPKIDIKIKDALGRAWQCTTIQFDFNEPERFDMTYIGADNSAHRP
                     YMIHRAVLGSVERFFATLLEYHSGNLPLWLAPVQIMLIPITDAQLNYARKIEEQLLEQ
                     GFRCELDSRSEKMGYKIRSAEKNKIPFMCILGKNEENNNQVTLRQHTVGDLGAMNLEA
                     AIAILRKEL"
     gene            complement(16491..18554)
                     /locus_tag="PLW08_01375"
     CDS             complement(16491..18554)
                     /locus_tag="PLW08_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPL51291.1"
                     /translation="MVIEEYDVHNLDCPDCGAKIEAEINNLEEVESANLDFINKHLTI
                     EYIEKVDNPLKRLNAIASKIDPNVQFTLAGLEQERTEKKPWFIIVAILVLTFSLFLSS
                     PFSLILGLIAYLIAGHRVIRKAVKEIASRQIFTENFLMTLATAGALYLGELIEASAVM
                     ILYEIGLYLESGAIAKSRKNLQALLALKPEKAHLKTENGIEDRKLNTIQKNQIILVYA
                     GERIPLDGVVIKGESTVDTSSLTGEAIPVFVEPKSTVFAGFLNGSGILEIQVQNSEAE
                     SMVSRIIKLIENASAKKSDTEKFMTRFAKYYTPAVVLAAILVFAIPSLLGYSYKIWLQ
                     RALIFLVVSCPCALVISIPLTYYIGIGKSAKSGIIFKGSIYLDLLSRVKTLIFDKTGT
                     LTTGELSIAEIKTVPDIDQEELKLSTWLCEWTSSHPFAKAVKKAFSYKFEPSKVDAFS
                     EFPGKGILLVYNQDRYLCGSDVFLESFGFLNLLDAGDNSVVHTVKNDIYLGCISFVDE
                     LKPGMKEVLTELKQKGVKHLSVLSGDRIPKVEKVAKELGLDSFYAALTPESKLTKLEE
                     IINSSQDIVAYCGDGLNDAPVLARADVGIAMGKIGAQASIETADVVLLNDKPEQLKSA
                     FSISKETNKMVWQNIFLALSIKMLVMILGIAGLAQLWEAIIADVGVTLLVIFNSLRMM
                     KHPDG"
     gene            complement(18555..18908)
                     /locus_tag="PLW08_01380"
     CDS             complement(18555..18908)
                     /locus_tag="PLW08_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPL51292.1"
                     /translation="MKRQNCLCKSISVEEMEDIKTHIPEDETILKMADFFKVFGDGTR
                     LKILYYLSRHELCVADLALLVQMQQSAISHQLKLLRLDRLVKYRKEGTTTYYSLDDEH
                     INSIFKVALEHLQER"
     gene            19865..20413
                     /locus_tag="PLW08_01385"
     CDS             19865..20413
                     /locus_tag="PLW08_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51293.1"
                     /translation="MHLLNRMVICTLTLFSFACANIPKSRTEEEKRIILPEGETSANC
                     ITEEELESFSFNTLECVQALLTGDGFTRFEKSGRPLIFLCPPETEPALQEQGKNFIGL
                     FCADLLQTQAVNLVVCDSVFTLSENREKILQLVKKSSADFFLQSIIAIEEGIPLLKLN
                     LYNVQNLAIVYSDTRSLLLIKI"
     gene            20426..21328
                     /locus_tag="PLW08_01390"
     CDS             20426..21328
                     /locus_tag="PLW08_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPL51294.1"
                     /translation="MQISKLDQMFDVLATRPKKRLVAAWGVDSHTILAAYMAVEMGIV
                     DATLVGDEKLILSVCREHNLDCSQFNIVHCPTDVKSAALAVEMINKGEGDFLMKGLLS
                     TDRYMKAILNKECGLMDPGAILSHVTVAEPRNYHKLLIVGDVAIIPLPDLNQKIAITN
                     YLIKTAHILGIENPKVGLLSASEQTLPKIPSSAEAAIIAKMAQRGQIKGAIVEGPLGF
                     DMIIDRESALIKGVDSPVCGDADCILFPNIEAGNTFYKTIIKLLKSELGAIVMGARVP
                     CVLTSRGDGERSKLYSIALAAMLA"
     gene            21331..22239
                     /locus_tag="PLW08_01395"
     CDS             21331..22239
                     /locus_tag="PLW08_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPL51295.1"
                     /translation="MITKLDELAEKAKNSGKKTRIAVAVAEDSNTINAILRATYEGFI
                     YPVLIGNEIKIKELLTSDIASNQFEIRNITEPNQAVKEAVGMVRNNEADVLMKGLIGT
                     DTFLKAVLDKEKGLLPPKAVMSYTCAIEIPKYHKLLFVSDTAVLIYPDLDQKIAMINY
                     SVAMARRFGIEKPKVALISATEKVSPSMQETLDYALLCKMAERGQIKNCIVDGPLDIF
                     LACDPNSLAIKGIQSPLEGDADILIFPNLESANSFYKGLMLFGEGELAGLICGTTKPV
                     IVMSRSESENSKYYCLALSCLMAEEK"
     gene            22236..22667
                     /gene="rpiB"
                     /locus_tag="PLW08_01400"
     CDS             22236..22667
                     /gene="rpiB"
                     /locus_tag="PLW08_01400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPL51296.1"
                     /translation="MKIAIASDHAGFEMKEAIKNAFPEIEFVDFGTHNTESMDYPDTG
                     FPAAKAVAEKQCEKGILICGSGIGMSITANKVKGIRAALCGNTDVARLSRLHNDANIL
                     VLAGRFTAIPYAIEITKVWLNTSFEGGRHLNRINKIQQGER"
     gene            22667..23959
                     /gene="glyA"
                     /locus_tag="PLW08_01405"
     CDS             22667..23959
                     /gene="glyA"
                     /locus_tag="PLW08_01405"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPL51297.1"
                     /translation="MNNIQKTDPEIYAAIMNELKRERENLELIASENFTSLAVLETQG
                     CVMTNKYAEGYPYRWSKKTGAINYNLYGRYYGGCEFINDAERLAIERAKQIFGAEHAN
                     VQPHSGSQANMAAYFTLVKPGDTVLALELSHGGHLTHGHPLSFSGQLYNIIPYTVNKE
                     TEQLDYDELEALAMEHKPQMILAGASAYPRKLDFARFREIADKVGAKLMVDMAHIAGL
                     VAVGLHQSPVPYADIVTSTTHKTLRGPRAGLILCKEEFAKEVDSKVFPGVQGGPLMHI
                     IAAKAVAFQEALQPEFKIYQQKVVENAETLANALIKKGFKLVSGGTDTHLMLIDLGPE
                     VDGSPSGKKMEEALDKAGITANKNTVPFDTRSPFVASGIRLGTPAVTTRGMGKAEMEQ
                     IADLIKSVYDHIDNEEYLQELKKEVHSLTDRFPLYPEL"
     gene            24318..25211
                     /locus_tag="PLW08_01410"
     CDS             24318..25211
                     /locus_tag="PLW08_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPL51298.1"
                     /translation="MRIAAIDCGSNSLKCLIAEKRGEDFNCLKDLRFQTRLASALNTN
                     GFLSATAIEKTITIFKHLQENEFPAYQIEEIRAVGTEAIRQAANKQEVVDRIFRETGI
                     QLSVINEAQETFLEWKGVLSGLKKPLQPITIFDSGGASTEIINGNKGLIHKATCFPVG
                     AVNFTQRYVHSDPISPTDFEELVRKIFQSLPQSFMIQDTFIGAGGGVYVCACVASGGK
                     INSPEEAEGYEITLSELERQINLYQYTNLEQRKQIPGMESEYADIMLAAVLIYWILLK
                     ASGKENFLVSTRGIRYGLVAI"
     gene            complement(25447..28005)
                     /locus_tag="PLW08_01415"
     CDS             complement(25447..28005)
                     /locus_tag="PLW08_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPL51299.1"
                     /translation="MKEYSNLKQGLTTKEVEEKLRREGYNELPGSKGRNLGKLIWDII
                     SEPMFILLVACAVIYFFLGDLQEALVLTASVVIVMSIELIQERKTEKALEALKNLSSP
                     RALVIRNGEQIRIPGREVVTDDLIILAEGDRIPADAYILENDLLSVDESLLTGESVPV
                     RKRIWDKKERPSTPGGDDLPFVYCGTLVVSGRGIAQVFATGERTELGKIGKSLETITE
                     EETRLSKETGRLVKYFAIFALLICAVVAIVFYITRKHLINALLTSITLAMSVLPEEFP
                     VVLTVFMALGAWRMSKKNVLVRRLQAIESLGSATVLCTDKTGTITYNKMTLQKIVSNS
                     ENIEIKNSAKLSEPILNTLIHSVLGSMREPFDPMEKAIHNALNDLDPFTYIELKNYQE
                     IREYPLTDELRAMAKVWKISGKTSYTVALKGSPEAVIKLCSVNAEEINTIMNQVSDLA
                     EEGLRVLGIASCEIETDVLPEKLTDLSFRYLGLIGLADPIRETVPEAVKICYKAGIRV
                     IMITGDYIGTAKAIGKQIGLNNVDNIISGSELDQMTDEELAEKCKQVSIFARVVPQQK
                     LRIVQALKANGEIVAMTGDGVNDAPALKAANIGISMGLRGTDVAREASAIVLTDDDFS
                     SIVSAVRMGRRIYDNLRKSFTYVFSLHIPIAGMAMIPASFADYPIIYFPVHIAFLELI
                     IDPACSIVFEMEKEEDDIMERPPRSVDEKLFGTKRLIIGFLQGLGVLILLVLMYFWGN
                     NRGMGENHLRAHIFTTLVFSNLMLITTNRSWHKNLFQIIRTPNKGMTYLFLGVFAIVL
                     LMIKVPSVTNIFHLETMSIMELLTCLILGNMSVAWFEIYKLIVNRKSAKKHLTE"
     gene            28214..29266
                     /gene="hrcA"
                     /locus_tag="PLW08_01420"
     CDS             28214..29266
                     /gene="hrcA"
                     /locus_tag="PLW08_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPL51300.1"
                     /translation="MKKNQLRLNETLKALVDEYIMSCEPVSSRILNEKYLRNVSSATL
                     RLDLLKLEKMNLISQPHTSAGRIPTIKGYREYLRLIEPEIEKIRYEGMDLLREILVHN
                     YKDTPRALYYIMENLAKETDQLSFVAEPEVSSGYLAKLDVFSIGENKLIFVMSLDSGL
                     DKTVILKCDNELTEAQLKKLVRYLNDELAGLQIYDIANRVLVEMQENADNGMISWFLA
                     ELHKAFMEISDFFIHYDGSINFLEQKEFNDKENILKFMSLLQRQDFLLNTMRKHQSDK
                     EVNIVLGEDLAKGSWLDFALIYAKYEVFGIPGYLGVLAPVRTEYRKLIPLIRDVANTI
                     TNTTRRGMVVPQEKKE"
     gene            29263..29820
                     /gene="grpE"
                     /locus_tag="PLW08_01425"
     CDS             29263..29820
                     /gene="grpE"
                     /locus_tag="PLW08_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPL51301.1"
                     /translation="MNKKIPKEPELEKEELITTEVSEEQKAKKIEELEKEVAEWKDKY
                     LRSMAEFENFRKRTISEKAEWIRLATQKFALEICDVLDNFERAIQQATEEEKSTPFGK
                     GVLMIEQQLRKALEKEGVKRIEALGEPFNPEFHEALAHIPSDQEENTVTAIIQNGYIM
                     NDKVLRPARVAVSNGSKINNEFQEE"
     gene            29821..31779
                     /gene="dnaK"
                     /locus_tag="PLW08_01430"
     CDS             29821..31779
                     /gene="dnaK"
                     /locus_tag="PLW08_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPL51302.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGSRTTPSVVGFTK
                     DGQRLVGQLAKRQAITNPQNTVFSIKRFMGRSYDEVTQEIKQVPFKVVRGLKNQAVVH
                     IDVENKDYAPQEISAMILTKMKETAEAYLGQKVTEAVITVPAYFNDDQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMENKKEQKVAVYDLGGGTFDISILDISEGVVEVLSTNG
                     DTHLGGDDFDKRVIDWILEQFKKQEGMDLSRDPMAMQRLREAAEKAKIELSGTLTTNI
                     NLPFITADATGPKHLNLDLTRAEFNRLTSDLVERTLGPCKRALDDAKLKPSDIQEVLL
                     VGGSTRIPAVQEAVEKFFGKSPNRSLNPDEVVAIGASIQGAILSGDEKVSDVLLLDVT
                     PLSLGIETLGGVMTKLIERNTTIPTKKSEIFSTAADNQTAVTIHVLQGERPMAADNKS
                     LGRFDLVGIPAAPRGVPQIEVTFDIDANGILHVSAKDKGTGKEQSIRIDRAGSISKED
                     IDRMIKDAELHSEEDKKRQEFVVAKNELDSLIFSTEKSLNEYGDKLSSSERSELEEEL
                     KNAKEQMERATEASELNNIKENLAKKAQHLGEVIYAHMQQQQGAGGEGYSQDASGFNS
                     GDYEQGPQEENPKQEGPIDADFEVVDDK"
     gene            31945..33102
                     /gene="dnaJ"
                     /locus_tag="PLW08_01435"
     CDS             31945..33102
                     /gene="dnaJ"
                     /locus_tag="PLW08_01435"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPL51303.1"
                     /translation="MAKRDYYEILGVDRNADEATIKKAYRKLAMQYHPDKNPNNKEAE
                     EKFKEASEAYEVLSDKEKRQIYDQYGHSGLENQFGGTGFSWEEFMHRSDLNDIFGDGL
                     GSIFETIFGGGFGGRNQRSSGNSNRGEDLQIELSLSLQEIANGVEKTIKIGIKEPCDK
                     CGGSGSAEGKTETCPNCKGTGQIRQIRQSLFGRMQTVSECPTCNGEGRIIKNKCSRCY
                     GEGRVGTVKEIPINIPPGVEDGQYIRFRGQGNAGPRGGSRGDLLVLIHQKKDDLFERE
                     GNNIILEYPITVSQAVLGDEIIVPTLTGSVKMKVPPGTQSGRLFRLKGQGIQGHNSYS
                     RGDQLVRTIVVIPTKISREEAELYNRLREFDRKRDLKPGKSFAAKLKSYFS"
     gene            33108..33812
                     /locus_tag="PLW08_01440"
     CDS             33108..33812
                     /locus_tag="PLW08_01440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPL51304.1"
                     /translation="MPSIYLPELSSEKKIITVEGEEFHHLINVKRIGIGESVKLNSGA
                     GLMAEGKLIKVNKKSAEIEIDKIETFPFPERPFAIAFALLKNRRDDWLIEKCTELGVI
                     AFYTMLTDYSVRTPSQNTITRFKRTALAAIKQCDNPWMPKINEPLPLKETVNKIFSEG
                     WIPVLCSERKPDKRITYLPKIIKPCFLIGPEGGFSEDEFTYFSEQNILSISICHLITR
                     AETAAIAVAAQYQAYF"
     gene            34023..34256
                     /locus_tag="PLW08_01445"
     CDS             34023..34256
                     /locus_tag="PLW08_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPL51305.1"
                     /translation="MNNKKLKILNPIMFVVILIAIIAIVTYKISEAFNYDAYGFKEIH
                     EICGFLFVILAIFHIIYNWQWIKSQIFKKKPKK"
     gene            complement(34692..35744)
                     /gene="lpxK"
                     /locus_tag="PLW08_01450"
     CDS             complement(34692..35744)
                     /gene="lpxK"
                     /locus_tag="PLW08_01450"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPL51306.1"
                     /translation="MDANKLIQNHLLKRSPLSYTLYPAGVIYGFLQKIRRTYYQRNEY
                     KPPCKIISIGNIVSGGSGKTPLTISLAKLLIKEGKKVAVSHRGYKGKFEYNPHLIADK
                     TGILYDVQHTGDEAYMIASSLPKIPVAVGRKRKEAIQLLLQNYPDLEIIILDDALQHY
                     YVHRDLDIISFDASLGLGNGFVIPAGYLREPISNIPSQSIAVINHKNEEQTQIPWLEK
                     IIRKGMPVFHCFSSPSCFKDAAGNTFPFSYIEGKRIVLTSAIANPASFEQSVCSLGLS
                     FMQHYAFKDHYAFSNTFLPQKLLELNPDFILCTQKDIMKLAKYEPLKSRLLALELEYS
                     MENQEGFVNQVLKSVR"
     gene            complement(35745..36122)
                     /locus_tag="PLW08_01455"
     CDS             complement(35745..36122)
                     /locus_tag="PLW08_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL51307.1"
                     /translation="MKTKKIICIVDDEEDLVENLKIELEAIRPNWKIITFSEGMKAIQ
                     EIVKGNVDLVVTDIAMPDMDGYELFWRIKDYNEKIPVIMMTGFGYDPNHVLVRAKVDG
                     LKDVLYKPFETEKLVELIESHLQ"
     gene            complement(36119..37000)
                     /locus_tag="PLW08_01460"
     CDS             complement(36119..37000)
                     /locus_tag="PLW08_01460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPL51308.1"
                     /translation="MYNEISQLPLVQQSRQGKVRDIYDLGETLLIVSTDRISAFDVVF
                     PTLIPDKGKILNAISVYFFNTTKDIVPNHFITADISEYPVELQRFGEYLEERSMLVKK
                     TRVIPFECIVRGYISGSAWKEYKKSNTIGGMLIAEELQESQKFPHPLFTPSTKAEKGH
                     DENISYREFMSRMDEPLAVYIKDKSLELYSYAHDLLLKKGIILADTKFEFGTIGSEVL
                     LIDELFTPDSSRLWSLEDYEMGKSPKSYDKQFIRDYLENSGWDKQSPPPPLPEEVIEQ
                     TRQKYLTILKIITGKEL"
     gene            complement(37010..37948)
                     /locus_tag="PLW08_01465"
     CDS             complement(37010..37948)
                     /locus_tag="PLW08_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51309.1"
                     /translation="MNEIKIDKNIQNEFDALPKGWVFYHIFGAGEVLYAGYSANLKAK
                     LKFFSNKAKEGGIYQELWNLAEKLSWETYPQAIFALIHYKCYLTENSPLYQYQIRPWA
                     DYVYLALDSHRFPFITIQEHTNDDWFYLGPFRSRFFLIDVLDIYARILKLPACETGEY
                     PCEKREKNLCRGWCLALDETSEKPTDYDLDKLESLLKEAFLVPENSIMELIQQERNKY
                     FNELEFDKSSLLDEDIEILNKYREWLKFLISAKHLEYESNELKIQKGKITYCRFQGKD
                     YYFPVDNIEFRINETLALNLNSVDESKVIYDFLQKM"
     gene            complement(38618..39418)
                     /locus_tag="PLW08_01470"
     CDS             complement(38618..39418)
                     /locus_tag="PLW08_01470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51310.1"
                     /translation="MKRLSLGKFFKGMNLTVFTLVMVFLYIPILILIIFSFNDAKIIT
                     GWKGFTFKYYVLLWQNKTILEAFRNTMLIAGLSTLISTVLGVLGALGLEYHKFSGRKL
                     FSALIYIPLVIPDILMGVSLALLFNFTRVNTGMLTVLIAHITFCISYTVIVIQSRLEG
                     FDYSLVEAAMDLGAKPATIFWKIKLPLIMPGIIAAALLAFTLSIDDFIITFFTSGRGF
                     DTLPIYVEGAIRRGTITTINSLSTIMIGLTLMLVIATKRIRKIVISSL"
     gene            complement(39415..40320)
                     /locus_tag="PLW08_01475"
     CDS             complement(39415..40320)
                     /locus_tag="PLW08_01475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51311.1"
                     /translation="MKIIPSDYPNSALQIAAERRKNFTSWILTAPALLWLIFFFLVPY
                     IIVIIYSFLTADIYDVKFEFTLDAYRQSFTPAYLHTFYLSFRLALLTTVLCLLLGFPV
                     AYYIARAKDKVKNTLLIFIIIPFWTNLIIRIFSWRIFLAYNGVLNNLLLSLGLISQPL
                     EIMRTDLAVILVMVYVYLPYMILPLYSVLEKLDWSLLNASMDLGANEVNSFFRITLPL
                     SKEGIFAGGLLVFIPALGSYLIPQLVGNQKSLYLGQVITYKVKNIPRNWPMASALSLT
                     LLLIVALFLLVIYFLYRKSKSRRQV"
     gene            complement(40317..41504)
                     /locus_tag="PLW08_01480"
     CDS             complement(40317..41504)
                     /locus_tag="PLW08_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51312.1"
                     /translation="MEEIRLENLSKDFDGFVAVENVSLAIQSGELFSFLGPSGCGKTT
                     LLRMIAGFEIPSTGRILIGNTDITDLPPYKRQVNTIFQNYSLFPHMTVFDNVAFGLRI
                     RKSPKNEIKNRVREMLSLVKMEDQANKFPEQISGGQKQRVAIARALINQPKVLLLDEP
                     LAALDLKLRQHMLIELMNIHDAVGITFIYVTHDQSEAMSISDRVAVMNYGNIIQEGPP
                     DDIYERPNSVFSAYFIGETNLISGEVISIEDDYVQIRCPDGTGNSFEIDSTFHFQPNI
                     GQELTVSLRPEKIAISRNKPRYQDDINILRGVIEDILYLGSHTQYIVRVGALKIRVFS
                     QHKRVYFDDTALDWEEPVWLFWHDTDSWLINEIPENVLTPEEISEGGVNHYQRSIKKP
                     AVQ"
     gene            complement(41755..43773)
                     /locus_tag="PLW08_01485"
     CDS             complement(41755..43773)
                     /locus_tag="PLW08_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPL51313.1"
                     /translation="MKKQRILRILLLCSVLCILSSTAFAHHNLPADKANAEIRFTETN
                     PPVAPVRPIAEFEPASDVLIRYPLGIPVSLVVQLANTANVICIVSSSQQSSAISTFTN
                     AGVNMERVSFLNAATDSYWTRDYGPWFIFDGNGEYGVVDFIYNRPRPNDNLIPQVFAN
                     HFALNYYGMNLQQTGGNYMSDGINTAAQTTLVYTENGNNQTNVNTKMQQYLGITNYLV
                     MADPNNTYIEHIDCWAKFLAPDKVMIRSVPANHSQYNAIENAANYFATHNCAWGYPYR
                     VYRVYTPSNEPYTNSLILNKKVFVPIVGSSNDNSALQAYREAMPGYEVIGVSQTSSAP
                     WESTDALHCRTHEIPDKNMLNIVHTPWHSIIPVGTDIIINTEIIAHSGQPLYTDSLFV
                     CYKVNSGAWQRNYLQPLTRNYYTVTLNGFAYGDTIRYFIHAADQSGRSVDHPVFAALD
                     PHLFVIQPDLTPPVLSHNPLSSISNQSEPISIIVSANDESGISQLLFRYKIDDSEVFA
                     YPMDPLTEDSYIFQYYPEFTTNDHTFYYSFMAYDGANPPNISFLPGQNLWYEVPILIV
                     SVNDEIQSPNIQEGIMAVYPNPFKAKGNDAIQLKYFSPQNIPFTFKVYNLKGQLIYTQ
                     TFFPEEKGIQEIKWNGRDNKGNLSPNGMYLIEILQGSKNYKSKLIIVK"
     gene            complement(43932..44624)
                     /locus_tag="PLW08_01490"
     CDS             complement(43932..44624)
                     /locus_tag="PLW08_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51314.1"
                     /translation="MPSHLYIIIGAYGSGKSEYSIHLARRFKTQGLDVVLADLDVVNP
                     YFRSREMQDDFIQEGIDIIAPEGEYKHSDLPMISPRVKGAIQNKAKTVILDVGGDPAG
                     CRTLGRFEEPIKTRGYNMLYIINTMRPFTSTPEEIISMKEALEATSRLKITEIICNTN
                     LMDETTVEIVLKGIKIVEKAAELSGLPFKTYLVLNKYEKIVPDNLGGKKREVMVYNLK
                     KPWELFVAKGIC"
     gene            complement(44699..45367)
                     /locus_tag="PLW08_01495"
     CDS             complement(44699..45367)
                     /locus_tag="PLW08_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPL51315.1"
                     /translation="MNNFQAVIFDMDGTLIDSMQLWRNVDREFLHTRGLIVPPDLFSE
                     LPQGNSFIQTAQYFKDRFGLPDSPESIMQEWTKMVSKHYETDIMLKPGAVELLSCLQE
                     KGIKIGLGTSNSLELAKKVLIRNSVWQYFQCAVTGDINLKGKPYPDIYLLAAERLEEK
                     PENCLVIEDTLTGVQAGKSAGMTVFAIYDEDSSDQHSLIKEIVDGFYWDFKSLAEDLF
                     RTWK"
     gene            complement(45408..45483)
                     /locus_tag="PLW08_01500"
     tRNA            complement(45408..45483)
                     /locus_tag="PLW08_01500"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45448..45450),aa:Gly,seq:gcc)
     gene            complement(45485..45572)
                     /locus_tag="PLW08_01505"
     tRNA            complement(45485..45572)
                     /locus_tag="PLW08_01505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45536..45538),aa:Leu,seq:taa)
     gene            complement(45585..45658)
                     /locus_tag="PLW08_01510"
     tRNA            complement(45585..45658)
                     /locus_tag="PLW08_01510"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45624..45626),aa:Cys,seq:gca)
     gene            complement(45671..45746)
                     /locus_tag="PLW08_01515"
     tRNA            complement(45671..45746)
                     /locus_tag="PLW08_01515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45711..45713),aa:Gly,seq:gcc)
     gene            complement(45799..46344)
                     /gene="amrA"
                     /locus_tag="PLW08_01520"
     CDS             complement(45799..46344)
                     /gene="amrA"
                     /locus_tag="PLW08_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPL51316.1"
                     /translation="MLTLEQKKELLSLARNAISARFKHQSVSLPEDPAFSPKRGVFVS
                     LHIGDNLRGCIGYIKGYKSIAESVKEMAEAAAFQDPRFPPLTEKELEKVTIEISILSE
                     LIPMQKNELPVIGKDGLYIQHPYGSGLLLPQVAVEYNWQPETFLREVCRKAGLPPNAY
                     LDPASVVYRFTADVFSEADTF"
     gene            complement(46363..47181)
                     /gene="amrB"
                     /locus_tag="PLW08_01525"
     CDS             complement(46363..47181)
                     /gene="amrB"
                     /locus_tag="PLW08_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPL51317.1"
                     /translation="MIRNPMHAGTFYPRFEQQIKRQIEGWISNAVTPLSSERALGVIL
                     PHAGYMYSGECATLGLHSISHENIDSFIILHPSHQANFFDFSVSPYQEYVNPLGTLDL
                     DMELYNKIAPEADQNIPLILHQEEHSMEIQLPILNYFFPKAKILPIMFGNQIPAVAKR
                     LADILYETIYSSTKRIVILCSSDLSHYHRARTAEEMDGILVKDVTALDPEHLWQDIIH
                     SDCEACGIGGILCLLYYAQHYTNAKMKVIQYTHSGKISGNDSQVVGYLSAKLYI"
     gene            complement(47178..48050)
                     /locus_tag="PLW08_01530"
     CDS             complement(47178..48050)
                     /locus_tag="PLW08_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPL51318.1"
                     /translation="MKYIIVIMIIIMNLTLLNADEYRIVDSRNSKTLTLQQMAKGLEK
                     YDIIFFGEFHDNATLHQLERELVPLLDTKRELILSLEMFERDVQSDLDAYIEGWLSED
                     EFLAKSRPWSNYQDDYRPLIEYAKQKKLTVIAANIPRSIAGKMARTGPDFTETLAEED
                     KKWLPDKIDYPDDGYKKAFLATLEDMHSPMMNNNPDWLYQAQCLKDETMAESIVNALK
                     MKPKARVLHFNGDFHSRNFSGTVSRVQSILPNKKIAVISPSYSENWQTANLTAEEKKS
                     GTYIIYLPLQGGEQ"
     gene            complement(48070..48663)
                     /locus_tag="PLW08_01535"
     CDS             complement(48070..48663)
                     /locus_tag="PLW08_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPL51319.1"
                     /translation="MLYTIEKKTDSEIKIKRSRFVAVLYPLPYVEEVKDILNEHYRLF
                     SDATHNCYAYIHGLNREIIYYSDAGEPTGTAGKPILNTLLRNDLTNILAVVSRYFGGI
                     KLGVKGLIAAYSEAVEQAILKSNLIEAKLLISFPLVCDYASFECLKHSFLAIGGEIKQ
                     VIWSEEVSFILNLPAEQEKEFRKIIDGNKHKIRLNYQ"
     gene            complement(48719..50008)
                     /locus_tag="PLW08_01540"
     CDS             complement(48719..50008)
                     /locus_tag="PLW08_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPL51320.1"
                     /translation="MNTTGLKNDNIVALVDCNNFYVSCERVFNPKLNNKPVAVLSNND
                     GCIVSRSQEIKDLKIPMGAPGFKYEGLIKKHGGTLLSSNYALYADMSSRVMEVLSLFS
                     PEVEIYSIDEAFLGLNGFKNRDLENFGKKIKRTVFQWTGIPVSVGISQTKTLAKVANH
                     FAKRYSAFQGSLCLLDDERIAKALAKTPVSEVWGIGRQYDKFLRQHKIETALQLRNAE
                     DKFIEHYLTSMGLKTVLELRGYACIDLDEAPANKKSIVTSRSFGKQVSSLNDLQEAIS
                     EYVTRAAEKLRKQKSVAGLLMVFLSTNRFKEGPQYNNSLSTTLFPPTAYTPELIKTAL
                     NLLDDLYLPGFEFKKAGVMLADIIPEKDVPLSFMDTNYLDDKRKKLMEAMDKLNRTYG
                     QDTLFYASSGIKKDWKMKRAKLSPHYTTHWNDLPKVK"
BASE COUNT        15820 a         9501 c         9361 g        15597 t
ORIGIN      
        1 agtttcaata atccaagcta attacctata ttgctatata ttagcaggat aaaatgtcct
       61 tctttttctc ttccttattc cgaaactcat aactattcac aaattagtgc tttcctaata
      121 gacaccgtaa cgactcccta acgactccgt aacacttccc taacacttcc ttaattttcg
      181 ttacaggatt gctacaaaat tgttaaaggg ctattgattt gtccagaaag gataaatccc
      241 tctggattca gctaacaaaa gacagcagaa ttcaatctgg gataatgaat aaggaggata
      301 atgttttggc aacttccaat ctgctaaaga gttcgcaaaa ctttgaaaaa aagcgttctt
      361 tttgtctaca ccaaataatg cattcggttt ttacaaaatt ggaattaggc aaattcagaa
      421 tacataatta tagttagaaa ccttcagttt aaatggtaaa gaggaaattg ctatcccatt
      481 ttatgttagg aattgataag ctcttcaagt tctgccagcc atttacggtt agctgattga
      541 attgctattg gctgtttgcc ggaatgtttg ccgaaatttt tcctgcggtt atcaaatagg
      601 caaatatttt cctgtaaaga ggtcatccag cgttcgctat ttaactgcag aatagaaaga
      661 tagatattaa gaaagagggt aactgaacta cggaaaaatt caccgggaaa atcggcagcc
      721 caaaacaata aaagatgggt aatatccttt tccggatagg caaattcact ttcggtaaag
      781 tcgataaaat aatactttcc ctggcagaaa agaatgtttt taggcgcatt atctttatgg
      841 cataagcact tatcgttttg gagagtagcc caatgaaagt tggcaatagt ttcagcaaga
      901 agagcaggtt ggaaattatt taattcatca agatagggga tacccgttat tttttccatt
      961 tttatccagt agggttctgc atagtccagc agttttggca acatcggcaa atttaattga
     1021 taggcaaaaa gttctgcctg aaagtgttta tagctactgt agtatttaat aacacaatca
     1081 ggcagaacaa taatagagct gaatttattc ttattcaagc tgaagacgga taatctgatt
     1141 atcgctgaaa agagggacaa tcaactgatt tgctggaaga tagtaaaaaa tatccgctgc
     1201 atctttaatg cctttgaaag tgggcagata gcgattttgt tcctgcggaa tttcaaaaat
     1261 catatcctga ccccaactgg tgatataaat tctgcccaaa tcatcaatcg caatgccgtc
     1321 caggtaatca taaatagtat ccttaaaaat gctgatgcgg ttatctaaag tgctgacaga
     1381 aagaatggga cttttttcct taaaacagac cacaaacatc tgatctctgg gcatatcata
     1441 aactatgccg ttgggttttt tcagcagagg ggaagtcaat ttttgcattt tattagctat
     1501 agggtctatc acatacacac agtccccgcc ggtatccgta gtgtaaataa ttcctgtttt
     1561 ggtaaaagcg aggtcgttta gcataacagc tccgggaatt ttgagggtat ctatgattac
     1621 cgccttttcc ggatcaacaa ttttgatagc gtttttatcc gccacataca gttttccagc
     1681 acgaatgata atgcctcgtg gttcttccag tccttcatct aaaaagggag aatactgttc
     1741 atccaaactt aaagcaacga tacttttacc taaagtattg gagatgagaa agcggtttcc
     1801 gaccgcgtca aaagcaatac tttcgggtcc gtttaacggg tctttcctgc tcagtaaaat
     1861 taaagtgata attgcagcta ctaaaaccag caggataaga ataagatata aggttttagc
     1921 tttcattttt tctcctttaa agtccatttc actccttcgg gagtgtcctt gatttcaatt
     1981 cccaactgag caaggtcatt cctgatttta tcggcaagag cccaatcttt tttgtctctt
     2041 gcttccttac gataagacaa tataagacaa ataaggtctt tagacaaatc cggcattttc
     2101 tgtttcaatt ttgcttccag attttggaaa aaccccagaa cagaacccaa ttccaccatt
     2161 tttatggcag cggaaattcg gtctgccata gttgcagagg tattttttac cttccgattc
     2221 agttcaaata aaagagaaat ggctttagca gtattaaaat catcatccat agccaaaata
     2281 aattcctgtt ccagattttg cagttcttgc gctgttccac aaacagttgg acgcctgttt
     2341 tccgcatttt ccggataatt gatttcggca agagaactat acaaattggc aacagcgcgc
     2401 tgtgattctt ccattaactc acggctaaaa tcaatgggac tgcgataatg cttggaaaga
     2461 aagaaaaatc tgattgattc ggcatcatat tcagccaaaa tatcacgggc cgtaaagaaa
     2521 tttttcaagc tcttgctcat tttttccccg tctacattta agaaaccatt atgcatccag
     2581 taattggcta agggctttcc actataagcc attgcctggg caagttcgtt ttcgtggtgc
     2641 gggaaaatga ggtctattcc tccacaatga atatcaaatg tctcaccaag atatttttga
     2701 ctcataacta cacattccgt atgccaacca ggacgtccct ttccccaggg actttcccaa
     2761 accggttctc caggtttgcc ttttttccat aaagtaaaat ctgcaggatg ccgtttgtta
     2821 taattttcca taactcttgc acctgccagc tgttcttccg tttttttacc cgaaagacag
     2881 ccgtaggaag gcaaattttc cgtagcgaaa tagacatccc cttccacttc ataagcataa
     2941 cctttctttt ccagttccgc aataaaggca attatatcat ccattacttc cgttgcttta
     3001 ggttgatagg ttggcggttt aattcccaaa gcggaagtat cttccagaaa agcacgagta
     3061 tatttttccg cgaccaaagt gaaactaata ttttcttcat ttgcctgagc aatgatttta
     3121 tcgtctatat cagtaatatt ttgaacataa gtaacttcgt aaccgcgata ggcaaaatag
     3181 cgacgaacta catcaaaaaa gataaaagcg cgtgcattac caatatgaaa gtaattataa
     3241 acagtaggac cacaggcata aatgcctact tttccagggg ttatgggctg gaattcttct
     3301 tttttacggg tttgagtgtt gtaaagtaac attctattat acctatttat agattaccat
     3361 tttttctgtt ttttcgacct ttttgccatt ggctgaacga gcttgaactt ttacaaaata
     3421 agtgttatta gctaaagtat cgcctttatc atcacgaccg ttaaaataga ccttattaaa
     3481 tccctgtttg ccataaatct taatattggc aatgcgtttg ccgcgaatag tgtaaattcc
     3541 tatatttact tcggcatcca tagaaagcat aaaggatata taaccatctt tggaaatagg
     3601 attaggatag atcagtaaat tttcaatagc cagttcggaa ctctttttag ctacaaaggt
     3661 ggtggttgcc acggcaggca tattaaaatt atcaaaagca ataatttgaa ttgtatgcat
     3721 tccttcactt aggtctttca ggggataaat caaagttccc gatgtgcagg aatttttatc
     3781 gtaattaaaa tattccgtaa ttgctatggg ctgtaaagaa ttatcaatta ccagaagaat
     3841 attatgcccc gaagaaccgg taatattaat gccgttggaa tcggaaatat ctgcatataa
     3901 ggtggtattt gtgccaacta tatccccggg acggaaatcc ttggaaccga gataaatgtt
     3961 aatttgcgga acatcaggat tggcaacatc cactgcttca tcgcttaact gcaaaggata
     4021 atagtaatta gaatagtcct ttttacctgt ttcatcccat aaataagcca caataagtcc
     4081 tgtggaacca cttacaatat catccggtac aatgaaagaa gaattataat gagaagaggt
     4141 aacttcactg cttccccgat agaattgatt tcccatttta gtaacattag tagcaggacc
     4201 aagagaatat gctcttttag aatcaaatgt ttttatttcc gttataccgg aaagatttga
     4261 gttagcgaac gaaccgttaa taacagccgt ttcacgcgaa tgcaaaatat cttcatcagt
     4321 ggaagttaca accagcatag tgctgtctcg ttccggtgga acaactctaa tcacaggatc
     4381 cccaaaaaga atatacattt cattattagt cgcaccacca gtataacgaa atttaccatc
     4441 cataatacaa taacccaaag gattacgatt ataagctaag ctttccaata catatttaag
     4501 caatattgag ctgttgtcag caaaactttt gcgggtagcc gacaaagaag ctatggctcc
     4561 taaattattc agtaaaacta ctttttgagc taaacaatca aaaccccaat aatcaaattg
     4621 agaaacctcg caggcagcgg caataaagaa agttagttta ccttcattgg caaatcttcc
     4681 catatcggta gctccgttca tatagtcctc agctccgagt ttatcatagg aaccatgccc
     4741 tgtataatgc ataagcaatc ttccttcatt gatggctttg aataaatcat ccctggcttt
     4801 gggcttattt tgaaattcat catattcata ttccatagca aaaatcttat ctacaaggtt
     4861 actgggatgc acatcattgc tgttgctttc agcaaattga gtgagaatat actcgtaatt
     4921 cgtatccccg tttgtaaggt catctgctat aaataccata gagttacgcc accaacccgg
     4981 agttggatta agagtataat tggtaaaatt ctgcaacata atgtttagct cattagtgtt
     5041 tttaacagga taccttccta tagctagttc tggataataa ctggtgttaa acattccgaa
     5101 ataatcatca gaaacgatat aacttccctg ccaaacaatc attttatttt taggggcgga
     5161 aaagccggaa aaattccgcc agtccatagt gccaatacct aacaaagtta aagaggtaat
     5221 gcggggtgca ggtaaattaa aataataata ctttaatgcc aaacggatag ctgcagggtc
     5281 tatatgtcca ccattaaatt gattataaat atcatctacc aaaatgacct tactgctaat
     5341 ttgatagatt tccctatatt tattggcaag ttcctgcgcc tgttgtaaaa attctgcagg
     5401 tgtcactatt atattatcaa actgatcgct ctcataggtt aaatcattgg gttgaactaa
     5461 ttgcattaca gaaggtctat atgcctctcc tggtttcagc ataaagtaaa atgtcttgct
     5521 gttaccatcg gcaataatac aattatcggc aacagggatt aaatctacac tgtaaagatt
     5581 attgatccga aaaacaaggg tattgctgag gtcgccactc aaattgaact tacaaatagt
     5641 gggaagcata gagtttttat ggaaaaattg cttttgagca ttacctttat ttaaattttg
     5701 ctgatagcta aagcgataat aatcaaaata aaggttatct ttactattgc ggaaaactct
     5761 tattttaaga gtatttatgc cattaatcag attacttata ggacgatcaa tataaaaata
     5821 attcgttcct gtccaggtaa aatccaaagt atgtaaatcc tgattattgt aaacaggcat
     5881 tccatttacc gatatttgaa ttttatgaac tgttccttct ccaactgatt cttggcataa
     5941 acgaagctga attttctgat catcactacc agagacatca gagagttctg tagtaagtat
     6001 ataatccgtt gtgttattcc cgaaaaaggt ttcactaaac caggtgaagc catatttttc
     6061 tcggcgatgg gtttctgttt ctatttgttt gataacagga gtagaagtta aagaaaaatc
     6121 ataagtgctt ggagaagaaa cagtttgaat tcttaaagga tttccactga agctacctcc
     6181 gaaagttaac cagaaaacct gattttgtga atagggattg taatataggg gatcggattg
     6241 aacttccgca ttctgctcat atccatcccg gaaactgcca taaaacaaaa tgtaatcctg
     6301 ttcgttaaaa ctgtggtctg cttctcctga aatataaata ggaatttccc tgaactcggc
     6361 tccgttatta tttatattgt tacctaagac cttgccggaa gtgctgaaaa tacgaatagt
     6421 agtaggatca atatcattta aaggcaaact gctaaggtcg gaataattaa ttctataaat
     6481 accaggacga ttagtttcta ttctcatcca ccagttactt aaaccaaagg gagcataatt
     6541 tattacggga cgaggattac tacgccagtt ttttgcttgc tgggaattga ttagttgcgc
     6601 caaaagaagg tctgctcctt tgtcgctttc ttccaaactg cgaaaattgg tctgacctga
     6661 aatattaatt ttgatagtag ctttggtagt tatagtaaga tttttgttgc catcgtaaac
     6721 aaaaggatgt agcgttaccg gcacaaaatg ataaccccgg aagtttgtgt ccggaagctg
     6781 aataataaat tctcctcttt ggggttgata tgcttcggga gagatattgt agaggtaata
     6841 atctacttct tcaccgcttt ttatttccgg aacagggata agtttggatt ccaaaataat
     6901 ggtagttgta ttactattca gtactgaaca attgatagag ccattgggag ggatacctac
     6961 tttaaattca gtgtatggaa tggaaggtgt tccgggaata ctgttgtgtc ccatattttc
     7021 agctatgatg cgactaaatt caccttccgc agtaattttg aaggattcta tagtatattc
     7081 aaccaataat tcggtttctg aactacggat aagggttatt ttggcattca gaaaagcaag
     7141 agaacaaaca agaataaccg ccagaaaaaa gcgtttcaag gtttctcctt aggtctgaat
     7201 aaatgctcca aagcatatat cagcagataa aacactgcca taaagataaa tatccccagc
     7261 ataccctgaa tcaatattct tagggcaagc gttatatttt ctatagtaga gacaagtaac
     7321 agtaacatta tttcctcttt ttatttatga tagttgcggt tattaattat agttaaagca
     7381 cggtaaatct gttcacatag aataagacga accaacctgt gcgtaaaagt aaatttggat
     7441 aaagataaaa tactgtttgc ccgttggcgg atagatttat ctacaccaaa aacaccacct
     7501 gttataaaga ccacattctt tctttcagat aagctaatca aaaaatcggc aaattcaagg
     7561 gaattttttt gttcgccttg ttcatccaac agaataacat aatcctcagg tttgatatat
     7621 ttcaggatag tagatgcttc tttggctttc acgctttctg cattacctga atgtttgatg
     7681 ctttcatcgg gcaattcaat aatctcaaat ttggcaaagg cagaaatcct tttccggtat
     7741 tccgagatac cttccagtaa ccatttatcc tttgttttgc caatttgcag aagggttaat
     7801 ttcactgttt ttttcaggac attgtagcta aggcaagtgc catacacagc attttggttt
     7861 cgcctgaatc tgcccgtgaa ggtatcaaaa taggcacttt agttccaata gttacatggg
     7921 caattttata gtgacaatag taggtaagca ttttgccatg cacatttccc gtttcaatat
     7981 tagggaagac caaaatatct gctgctcctg caacttcgga agtgagacct tttatgcgtg
     8041 cagcttcttt atccactgca ttatcaaaag ctaagggacc ctctatgatg caaccgggaa
     8101 tttgacccct ttcattcatt ttagcaatca aagccgcatc aatggtagct ggcattttgg
     8161 gattgactgt ttctatagcg caaatcatag ccactttagg tttgggattt cctaaggaat
     8221 gagcaacctg cacagcgttt ttaacgatag ctattttctc attcagttcg ggcaggatat
     8281 taattccacc gtcagtttga atttttagtc cttccggatg ttcataaaca aaaacatcgg
     8341 aaattacctc gcttactttc aagccctttt ccttatccaa tgcagctttt aggataacgg
     8401 aggattccat tttccctttt aatatgaggt cacctttttc ttctttggct aaagcaacgg
     8461 cagtttcagc tgcttttctc aaatcactgc cggtatcaat aatttcaaag gcgtttacca
     8521 gatcgggagc tttctgttgt atcagacgga taatttctgg tttatcgcca accaggatac
     8581 tttcacataa gccctctttt tttgccataa cacctgcttc tatggcagat tcaatttggg
     8641 ctccggcaat aatagcagtt cctttacggt tttgttttac atagcttagc aattcggcaa
     8701 aattcttaaa catactgata tatctcctct tttttagtta aaatgaaaaa attatctgcc
     8761 ctaactgggt agaaagaaaa caggtgctta ataatgatac ttttccagaa acctatctac
     8821 aaattcatct tcttcgggtt ttatttcccg cataattttc tttaaagctt ccttttgaat
     8881 ttggcgaact ctttctctgg ataaccctaa tttttcagct atttgggcaa aatttcggga
     8941 ttcgtgggat tcatttaagc caaaataatt cctgataata tctgcttcac gggcatccaa
     9001 tttactgatc gcattctgaa ttttttcctg cattctttcc tgatagtata aactttgggg
     9061 gtctaaggta tctttatctt ccagcaggtc tttagcagta aaatcctgac tatctgaagt
     9121 agtaataata tcatcaaaag agcttgtttc cggtaacttg ttttgcagtt gtgctatggt
     9181 tttttcggca atattcattt tctcgcttag ttcatcgggg gtagctattt ttcctgtttc
     9241 cgtaaaatat ctgtcttgag taagtttaac tctgtgagct gcgctggatt ttcccattgg
     9301 aacgcgaata agagaacttt tttcgctaac ggcaagcatt attctctgtt ttatccacca
     9361 gatagcatag gaaataagct taatatcttt atcgggatca aatttttcta tagctttaat
     9421 aagccctata ttgccttcgc tgattagttc cgaaagggat aaaccccgat tttgatagcg
     9481 tgaagcaatt ttaactacaa atttcaaatt tgcctggatt aacttgttca ttgcatccac
     9541 atccccgttt ttagctttaa tagcaaggtc gtgttcttcc tcacgggaaa gggttttgaa
     9601 cttggaaatc tcgttcagat aattttgcaa tgctttatcc gcaaaaacac tttctttatt
     9661 gctcattttt tattccttag ggtaaaggaa gaactttacg ggggttaata gctttacctt
     9721 tcaaacggta ttcaaaatgt aaatgagtgc cttgagcatt gccggttgaa ccaactgtac
     9781 caatttgctg accttgcttt actacatcat ttacatttac cagatttttc tcgttatgag
     9841 cataaacagt catcacaaaa tcgggatgtt caataacaat tacattacca taagaacctt
     9901 gaactcccga ataaacaatt gtaccatcta aaacagcata aattggcgtg ccggctttag
     9961 cacctaaatc aattccttta tgaggacgac cattgcgcaa tccatattcg gaaataactt
    10021 ttccttccac gggcataatt aaatcggtgc ggattttttc ccgtttcata atttcttctt
    10081 cagttagaac ttccggagga gtagttattt ccggacgcgg tttaccagaa agatataatt
    10141 tctgtcctac tttaatatct ggagaagtta aattattcaa ttgcatcagt tcttcaacag
    10201 tcattccatt attggtggca attctgtaaa gtgtatcttt tttttggact acatagaccc
    10261 tttcaataag caccgtatct tttttggcag gaaccgtaga gataacagga ttggattctg
    10321 taattatttc ttcaggaaca attttggttt cgggtaattc cgcaggattt ttaatcacca
    10381 gtttatcacc tgggtgaata ggaattgagc tattggcaag gttattccaa gcaagaagct
    10441 ctcctaatgt tatattatgg tttacggcta tacgatagag gttatcaccg gttttaacag
    10501 tgtaataata atccgcggga atttttttgg caggggagag ggaaggatta gtttctgcac
    10561 ctggcaaaga agaaatctgt ggggaagaag tacctcctga aatttctgga gtaacaggtt
    10621 tggtaactgg aacgggagca accggtttaa ttggttccgt atctttcttc acgattaatt
    10681 tttgaccgat ggaaagattg ttatcggata agttattaag ccgtttcaat tcatccacag
    10741 ttgtgccata ccgcttactt agagaataca gggtatctcc tttttttatt atatgtatct
    10801 ggtcctccgc agataaaccc aaaggtaaaa ttagcattat ctgcattata accagccaca
    10861 gtaatctatc taatttcaaa gtcattatat ttttcaacgc tatccaattt ataaatttgc
    10921 agttctttca cttgagcata tttggttcct tgcgaaacga tctgttgaaa ggtctgcaaa
    10981 gtgtgttctt ctgcctgggc aacgatttta accgtgccgt cccactggtt ttgaacataa
    11041 cctttaattc caagttcgga agcaattttc catacataat aacggaatcc tactccctga
    11101 actctaccgt ttgcataaag ttcccaagta ggcatatatc acacctttag attatttgtt
    11161 ctgcataaat tttatgcccg aatttgtcaa gtattcgttt tctaccattt ttgaaaaggt
    11221 gatatttttc cttatatcaa gcaaaaatat acttgaccat aacagcgagg ttaaaaatat
    11281 atagacatta tggttaaaag gattgtaata attggatgaa cttattgcat atcattaact
    11341 tagtaggggg cttggcactt tttcttttcg gcatcaataa aatgagcgat agtttacaag
    11401 cagttgccgg ttcggaaatg cgtcggattt tgaaagtgct gattaataca cctttaaaag
    11461 gggtcttagt gggtttgggt gtaactgttt taattcaaag tagttctgct accaccgtta
    11521 tgatgatagg attggtcaat accggaatta tgactttgaa tcaagcagtt ggtgtagtta
    11581 tgggagccaa tattggaacc acgattacag ctcagttaat tgcattcaat ataggacagt
    11641 atgctttttt atttgttatt ttaggggtaa ccttactttt catccgcaaa agcaaaacaa
    11701 tggaacattg gagtcagatt cttattggtt ttggtttgtt gtttgtaggg ttaaatgtga
    11761 tgggtaattc ggtttctcct ttacagaatt ctattactgc tcgtaatttg atgatcagat
    11821 tagcttccaa tcccatacta agtgttctgg taggaacctg tttcacgatg ttaattcaga
    11881 gttcttctgc atctatagga attgttatgg tattggcatc aacaggtctg ataccatttg
    11941 aaggggcttt atatcttgtt tttggagata atatcggcac aacaataact gcctggctag
    12001 cagcatcaag tagtggtaat acagcaaaac gggtagctat ggtgcatact cttttcaatg
    12061 tatttggcac cattattttc ggcattttaa cttatctggg gatttatact cttttaatca
    12121 attggattac tccgggaaat gtttatgccg gacaaaatat agcccgtcat gttgctaatg
    12181 gacatacgat gttcaatgtg attaatatga tattattttt accctttgct aatcagttgg
    12241 caaatctggc aacgaaaatt attcctccgg ataaagtgga aaccatttct ttaggcgaac
    12301 ctaaacattt gaattacacc attatcagca attccgacct ggcaataaac cagtccatca
    12361 aagaaatgcg cgaaatgctg cgtttggtta gaatggaact tatgcttgcc tatcaggcat
    12421 ttaaggaaaa agattataaa aaacagatgc gggtttcccg aattgaaaaa gcaattgata
    12481 atctacaaag agaaataact ctttatctgg tagcagtaaa tgaaaaaacc aatgcggaag
    12541 atattattca taaaattccg gcacttctgc acactgtaaa cgatattgaa aaaatcggtg
    12601 attttaccga agaaataaac aaaatcctga acgaacagat aatagctaaa aagcatcatc
    12661 tttctcctaa gtttatttcc ataattgatg aattacattc caagctgctg tatatgttgg
    12721 atttatctat tgactacctg gtagagttga aagaggatta tatctataag atcattgaaa
    12781 tggaaggacg catcaatgag acacatcatc acctgcgtga ggaaattctt tctcaggttc
    12841 aaaatggaaa atgcaatgca gcagagggtt taaatactat tgattatatt gatgaagtgg
    12901 aagaaattgc cgataaactg aaaaatttgg taaaagcagg aactcataat tttgtttata
    12961 atcgtaattt tgtgaaagaa actgaggaag aagaaaaaac ggaaaacgat aaagaattaa
    13021 ccgatgtttt ttaaataacg ccagctgtta tcaaatcgtg catatgtaac attcctatag
    13081 ggacattatt ttcatccact acaggaatca tcgttatttt ataggtctcc atcaaattta
    13141 atgcttcaat agctaacacc tcaggtttca aggttttagg attggctgtc atacagtcct
    13201 ttgccgtata gcttaaaaga gaattacctt tattgtgcag ttgtcttctt aaatcaccat
    13261 cggttatcat tccggtcaat tttccatcct tattagtaac tgcagtacag ccaagttttt
    13321 tggaagtcat ttccattatg gcttcggaca tttttgcctt ttcttcaata accggaagtt
    13381 cctttccact atgcattaaa tcggaaactc ttaaaagcag ttttttaccg attgtaccgc
    13441 caggatggaa ctttgccaaa tccttcagtt ggaagttttt atgtttcagt aaggcaatag
    13501 ctaaggcatc tcctacagca agagctacag ttgtggaagc agtagggact aatcctaagg
    13561 gttccagttc tttaggaata tgacaattga gcactacatc ggaatttttt gccagttgtg
    13621 aatttggttc tccggttata gctattatag gaacatattt gaattttaaa aaaggaatta
    13681 aatttatcag ttcctgtgta tttccgctat tggaaacagc aattactaca tcaccggatt
    13741 caatcatacc taaatcgcca tgaatacctt ccgcggcatg caaaaaaatg gaagttgtac
    13801 ctgtagaagc taaagtagca ctaattttac gggcaataat accggttttt cccattcctg
    13861 ttaagaccac ttttccttta catttgcaaa ggaggtcaaa agctttttcc aaggattctt
    13921 tactaagttg ttttgctact ttttgaatag cggttgcttc tttagcaagt tcttcctgaa
    13981 cagtttcgta aatattcata attcttttct caagatagcg attgctgctt ccaaattcat
    14041 agcaccaaga tcgccaacag tatgttgacg caaagtgacc tgattattat tttcttcatt
    14101 ttttccgaga atacacataa agggaatttt attcttttcc gcagaacgaa ttttgtaacc
    14161 cattttttca ctgcgtgaat ccaattcaca acgaaaacct tgttccagta attgttcttc
    14221 aattttgcgg gcatagttca attgagcatc ggttatagga atcagcatta tttggacagg
    14281 agcaagccaa agcggtaaat taccgctgtg atattccagt aaagtagcaa aaaatcgctc
    14341 tacagaaccc aaaacagctc tatgaatcat ataaggtcta tgcgcagaat tatccgcacc
    14401 aatataagtc atatcaaatc tttcgggttc attaaaatca aattgaatgg tggtacattg
    14461 ccaagctcta cccaaagcat ctttaatttt aatatctatt ttaggtccgt aaaaagcacc
    14521 accaccttca tcaactttat attccaaacc caatttttgt aaagaattag ctaaagaagc
    14581 ggttgcttta tcccaatcct ctttttctcc aacacaatca tccggtttag tggcaagata
    14641 aattaaaaat tctttaaaac cgaatgcctt cagcatatcc agagagaaaa caatcaattt
    14701 atctacttcc atatccatct gttcgggagt gcaaattata tgtgcatcat cttgagtgaa
    14761 accacgaacc ctcattagac catgtaaaac accacttcgt tcatatctgt aaacagttcc
    14821 cagttctgcc aggcggatag gaagttctct atagctgtgt aagctggaat tataaatact
    14881 aatatgaaaa ggacaattca tcggtttgat gtaataatcc tgaccttcaa tatccatttt
    14941 gctatacata ttttctttat agaaatccaa atgaccgctg gtttcccata aattagcgcg
    15001 tcccaaatga ggagtataaa ccaattgata gccctttttc aggtgctgtt ctttccagta
    15061 tgcctcaatt aaatgacgca ccatagctcc tttgggatgc cataaaacca aaccaggtcc
    15121 aacttcatcg cttatagaga aaaggtccag gtctttgccc agttttctat gatcgcgttt
    15181 acttgcctct tccaggaaat gaagatagtt cgtaagctct ttttccgtag gaaaactgat
    15241 tccatatatc cgtttcagca ttttgttttt ttcatcacca cgccagtatg cacccgaagt
    15301 tcttaataac tttatggctt taatttttcc ggtatcggga agatgaggtc cgcgacaaag
    15361 gtcaataaaa tcaccttgac ggtaggagct gatgatttct ccttccggaa tatcttccag
    15421 aagttccagt ttgtatgttt cgttcatctc gctaaatagc tttattgcct ccgctttact
    15481 taattctgtt ctgtaatagg gaagggcttg tgtgcttaat tcttgcattt ttgcttcaat
    15541 tttttgcaag tcctcttccg taaaggattc gtctctatca aaatcataat aaaaaccctg
    15601 ttcaatagca ggaccgatgg caattttcac ttccggaaac aattgtttca ctgcctgagc
    15661 cattaaatga gccgtactgt gccaataaac ctcttttcct ttttctgtat tgaatgtatg
    15721 aataactact gtagcatccg tatctatagg atagtttata tctaccattt tgttattaac
    15781 ttctgcacaa attgcctctt ctgcaagttt gggaccaata tcttcggcaa tttttaaagc
    15841 tgtaacagga gctgaatatt ctcgtatact accatctggc aaggtaatct tgatcattta
    15901 tatctccatt atataattct ttgcacaaaa ataacctttt tttccataaa taatattctg
    15961 tcaagctgat taaagggatt ttacttttac caagagaaaa agagaaaaga gattggattt
    16021 tacaaagaaa ttatttaagg tgtagatgga agacgctatt cctccgccaa aagaaaaaaa
    16081 tgcggagctt cagcaagctc ttactacaca atcatcacag ctataaaaag ttttccatct
    16141 ttatataatt tgtaatcacg atgcagttcc caaaagttaa ccttaatcgc tttgcgtgat
    16201 aactgccagc gtagttgtta atcagctaaa aattatcagg tgtaagttat ttccgatttt
    16261 gttgttctct tgaagcagta atgatcaagt aacaaccaag taacaattcc ctaacgagac
    16321 aacggaagtg ttacggaagt gttacggagt cgttactgaa tgttctaaat atcaataagt
    16381 tagctatcaa catttaaggg aggaagaaaa aggacatatt atagatttta tctcttctat
    16441 atcaatggat tagggaatat taaatcaaat aagaatggtt gataaagatg ttaaccatcc
    16501 gggtgtttca tcattcttaa ggaattgaaa ataaccagta aagtgacacc tacatcggca
    16561 attattgctt cccagagctg agccaaacca gcaattccca aaatcatcac cagcatttta
    16621 atgcttaaag ccaggaaaat attctgccag accattttat tggtttcttt agaaatgctg
    16681 aaagcgcttt ttaattgttc aggtttatcg ttcagtaaaa ccacatcggc tgtttcaatg
    16741 gatgcctgag cgccaatttt tcccattgcg attcccacat ctgctctggc taaaacaggt
    16801 gcatcattaa gaccatcacc gcaataggca actatatcct ggctggaatt tataatctct
    16861 tccagtttgg ttaatttgct ttccggggtt aaagcagcat aaaagctatc taaacccaat
    16921 tctttggcaa ctttttcaac tttgggaatg cggtctccgg atagaacact caagtgtttt
    16981 acgccctttt gtttaagctc tgttagcact tctttcattc ccggtttcaa ttcatctaca
    17041 aaactgatac agcccaaata aatatcattt ttaacggtgt gaaccacgct gttatcgcct
    17101 gcatcaagca aatttagaaa accgaaggat tctaaaaaga catcacttcc gcaaagataa
    17161 cggtcttgat tgtaaaccag caaaattccc tttccgggaa attcggaaaa agcatctacc
    17221 ttggagggct caaatttgta agaaaatgcc tttttaaccg ccttcgcaaa aggatgtgag
    17281 gaagtccatt cgcaaagcca ggtacttagt ttcagttctt cctggtcaat atccggaact
    17341 gtttttattt cggcaatact caattcaccg gtggttaaag ttcctgtttt atcaaaaatt
    17401 agggttttaa ctctgctaag taaatccaaa taaatgctcc ccttgaaaat gatacccgat
    17461 tttgcggact tacctattcc tatataataa gttaaaggta tggaaatgac taaggcacaa
    17521 ggacaggaaa caaccagaaa aattagggca cgctgcaacc agattttgta tgagtaaccc
    17581 aaaagggatg gaatagcaaa aaccagaatt gcagctaaaa ccacagcagg tgtataatat
    17641 ttagcaaaac gggtcataaa cttttcagta tctgactttt tggcactggc attttcaatt
    17701 agttttataa tgcgagaaac catactttcc gcttcactat tttgtacttg aatttccagg
    17761 attccgctgc cattcaaaaa tccggcaaaa acagtggact ttggttctac aaaaacagga
    17821 attgcttctc ccgttaaaga tgatgtatca accgttgatt ctccttttat aacaaccccg
    17881 tccaaaggaa ttcgttcacc tgcataaacc agaataatct ggtttttttg aatggtattc
    17941 agtttgcggt cttcaattcc attttccgtt ttcagatgtg ctttttcggg ttttaatgcc
    18001 agaagagctt gcaaattttt ccggctttta gcaatagctc cgctttccaa atataatcct
    18061 atttcatata aaatcataac tgccgacgcc tcaatcagtt cacctaaata taacgctcct
    18121 gctgtagcta aagtcatcaa aaaattctcg gtaaatattt ggcgggatgc tatttccttc
    18181 actgcttttc taataaccct gtgaccggca ataagatagg caattaaccc taaaataaga
    18241 gagaaaggac tgcttaaaaa caaactgaaa gtcagcacca aaatagctac aataataaac
    18301 cacggctttt tttccgtcct ttcctgttcc aaacctgcca gagtaaattg gacattagga
    18361 tcaatcttgc tcgctatggc atttaagcgt tttagaggat tatctacctt ttcaatgtat
    18421 tcaatggtga gatgtttatt aataaaatcc aaattggcac tttctacttc ctccaaattg
    18481 ttgatttcag cttctatttt agcgccgcaa tcgggacaat caagattgtg aacatcatac
    18541 tcttcaataa ccatttatcg ctcctgtaaa tgttccagcg ctactttaaa aatgctgttt
    18601 atatgttcat catccagaga ataataggtt gtagtgcctt ctttgcgata tttcaccaga
    18661 cgatccaaac gcaataattt aagctgatgc gaaattgccg attgctgcat ttgaaccaac
    18721 agggctaaat ccgctacaca caattcgtgt ctggaaagat aatacaatat tttcaaacgg
    18781 gttccatccc cgaaaacctt gaaaaaatca gccattttta ggatggtttc atcttccgga
    18841 atatgtgttt ttatatcttc catttcctct acggaaatgc ttttacataa acagttttgc
    18901 cttttcattt cagctccttt atgaatatat gttcatatat tgataaaccc gcttccttgt
    18961 caagaaaaat ctgcgcagga aaaatgggtt ggcaacttcc tatttgccaa ggagttcgta
    19021 gcactcagag aaataaatgc cttctgttta tggcgaatca taattttgca gccaaaatgc
    19081 taaatttgat tctcttttct ccaaggttaa aacctatagt taatttctgc tgttcctttg
    19141 ttctatctgc tcaggtagaa atccagaaac aaacaatgta gccatatttt tccaccgggt
    19201 taaaacccgt cgttaatacc tgtcgttcct acggaactcg ttctcagaaa aagagataag
    19261 cattatccta ttatcctttc gccgggttaa aacccgccgt taatatctgt cgttcctacg
    19321 gaactcgttc tcagaaaaag agataagctg tatcctatta tcctttcgcc gggtttaaaa
    19381 cccgtcgtta atatctgtcg ttcctacgga actcgttctc agaaaaagag ataagctgta
    19441 tcctattatc ctttcgccgg gtttaaaacc cgtcgttaat atctgtcgtt cctacggaac
    19501 tcgttctcag aaaaagagat aagctgtatc ctattatcct ttcgccgggt ttaaaacccg
    19561 tcgttaatac ctgtcgttcc tacggaattt ttttcagcaa accttatact tttcaccatt
    19621 cacctttcac tattcaccgt tcactattca atattcacct tcgcggaata tttttcctta
    19681 aaatcctact cataacgctt cttatgatta agctaactta acgccagtta gaaaattagg
    19741 aaaactcgga aaacaaatct tgacagaatt aggaattaat aacataaaga actaaattat
    19801 tttgagacgc tatgaaatga agttaatttg ccttactgca tacagttgcg ctaaaaggaa
    19861 gaaaatgcat ttattaaacc gaatggttat ctgcacttta actcttttta gttttgcttg
    19921 cgctaatatt ccgaaaagca gaacggaaga ggaaaaaaga attatccttc ctgaagggga
    19981 aacttctgca aattgtatta ccgaggaaga attagaatcc ttttctttta atactctgga
    20041 gtgtgttcag gcattgttaa caggggatgg gtttacccgt tttgaaaaaa gtggcagacc
    20101 gcttattttt ctatgtcctc ctgaaacaga acctgcttta caggagcaag gcaaaaattt
    20161 tatagggtta ttttgtgccg acttactgca aacacaagct gttaatctcg ttgtttgtga
    20221 ttcagttttc actttatcgg aaaatcggga aaagatattg caattggtaa aaaaaagtag
    20281 tgcagatttc tttttacaaa gtataattgc aatagaagaa ggaattcccc tattaaaatt
    20341 gaatctctat aatgttcaga acttggcaat tgtttatagt gatacccgca gtttattatt
    20401 aataaagata taaaggatga aaactatgca aattagcaaa cttgaccaaa tgttcgatgt
    20461 tttggctacc aggcctaaaa agcgtctggt agctgcctgg ggtgttgatt cccatactat
    20521 tttggctgcc tatatggctg tagaaatggg gatagttgat gctaccttgg ttggcgatga
    20581 aaaactgata ctttccgttt gccgggaaca taatcttgat tgttctcaat ttaacattgt
    20641 ccattgccct actgatgtta agtccgctgc tttggctgta gaaatgatta acaagggtga
    20701 aggcgatttt ttaatgaaag gtcttctttc cacagatcgc tatatgaaag caatattgaa
    20761 taaggaatgc ggattgatgg atcctggtgc tattttatcc catgtaacag ttgccgaacc
    20821 gagaaattat cataaattac tgattgttgg tgatgtagct attatacctt tgccggattt
    20881 gaatcagaaa attgccatta ccaattactt aattaaaacg gctcatattt tagggataga
    20941 aaatccgaaa gtaggacttc tttccgccag cgaacaaacc cttcccaaaa ttccttcttc
    21001 cgctgaagca gctataattg ccaaaatggc tcagcgcggt caaatcaaag gtgcaattgt
    21061 agaaggtcct ttgggatttg atatgattat tgatagagaa agtgctctta ttaaaggtgt
    21121 agatagtcct gtttgcggag atgcggattg cattctcttt cctaatattg aagcaggcaa
    21181 tactttttat aagaccatca ttaaactgct gaaaagtgaa ctgggagcaa tagttatggg
    21241 tgcgcgggtt ccctgtgttt taacttcccg gggtgatgga gaacgcagta aattgtattc
    21301 tatagcttta gcggctatgt tggcgtaaaa atgattacca aacttgatga actggcagaa
    21361 aaagctaaaa acagcggcaa aaaaacccgc attgcagttg ccgttgcaga agatagcaat
    21421 accattaatg ctattttacg agctacttac gagggcttta tttatcctgt tctgattggc
    21481 aatgagataa aaatcaaaga attactgacc tctgatattg cctctaacca atttgaaata
    21541 aggaatataa cagaaccaaa ccaagctgta aaagaagctg ttggaatggt tcgcaataat
    21601 gaagccgatg ttttaatgaa aggtctgata ggaaccgata ctttcctgaa agctgttttg
    21661 gataaagaaa aaggactttt acctccgaaa gcggttatga gctatacctg tgccatagaa
    21721 attcccaaat accataaact gctttttgtt agcgatacag ctgtgttaat ttacccagat
    21781 ttagaccaga aaatagcaat gattaattat agtgtagcta tggcaagacg ttttggtata
    21841 gaaaaaccga aagtggcatt aatatctgcc accgaaaaag ttagcccttc aatgcaagag
    21901 accttggact atgctctttt atgtaaaatg gcagaaagag gtcaaataaa aaactgcatt
    21961 gtagatggtc ctttagatat ttttcttgcc tgcgatccta attcccttgc tattaaagga
    22021 attcaaagtc ccttggaagg ggatgccgat attcttattt tccctaatct ggaaagcgcc
    22081 aatagttttt ataaaggtct gatgcttttt ggagaagggg aactggcagg tttaatttgt
    22141 ggaacaacaa aacccgtcat tgttatgagt agaagcgaaa gtgaaaactc taagtattac
    22201 tgtttagcgt tatcttgttt gatggcggag gaaaaatgaa aatagccatt gcttccgatc
    22261 atgccggttt tgaaatgaaa gaggcaatca aaaatgcttt tccggaaatt gaatttgtgg
    22321 attttggcac tcataataca gaaagtatgg attatcctga taccggtttt cctgctgcta
    22381 aagcggttgc ggaaaaacaa tgtgaaaaag gtatcttaat ttgtggcagt ggaataggaa
    22441 tgagcattac tgccaacaaa gtgaaaggta taagagctgc tttatgtgga aatacagatg
    22501 tagcacgtct ttcacggctg cataatgatg ccaatatttt ggtcttggca ggaagattta
    22561 ctgccattcc ttatgccata gaaattacta aggtctggct aaacacctct tttgagggag
    22621 gaagacattt aaatagaatt aataaaatac aacaaggaga aaggtaatga acaatattca
    22681 aaaaaccgat cccgaaattt atgccgctat tatgaacgaa ctaaaaagag aacgggaaaa
    22741 tctggaactt attgccagcg agaattttac ttccctggct gttttggaaa ctcagggatg
    22801 tgtgatgacc aataaatatg ctgagggcta tccctatcgc tggagcaaaa aaaccggagc
    22861 tattaattac aacctttacg gaagatatta tggcggttgt gaatttatca acgatgctga
    22921 acgcttggca attgaacgtg ccaaacaaat ttttggagcg gaacatgcca atgttcaacc
    22981 ccatagcggt tcccaggcaa atatggctgc ctattttaca cttgtaaaac caggtgacac
    23041 ggttttagct ttggaactat cccatggagg tcatttaacc catggccatc cactttcctt
    23101 cagtggacag ctttataaca ttattcccta cactgtaaac aaagaaacgg aacaattgga
    23161 ctatgatgaa ctggaagctt tggcaatgga acataaaccc caaatgattt tagccggcgc
    23221 ttctgcctat ccccggaaat tggattttgc cagatttcgt gaaattgccg ataaagttgg
    23281 agctaaactt atggttgata tggctcatat tgcaggactt gttgctgtag gtttacatca
    23341 aagccctgtt ccttatgcgg atattgttac ttccacaact cacaaaacct tacgcggacc
    23401 tcgtgccgga cttattttat gcaaagaaga atttgccaaa gaagtggata gtaaggtttt
    23461 tcctggtgtg caaggcggtc ctttaatgca tattattgct gctaaagcag ttgcttttca
    23521 agaagccctg caaccggaat ttaaaatcta ccagcaaaaa gtggtggaaa acgctgagac
    23581 cttagccaat gccttaatta aaaaaggatt taaactggtt tcaggtggaa ctgatactca
    23641 cctgatgctg attgatttag gtcctgaagt tgatggcagc cccagtggta aaaaaatgga
    23701 agaagcactt gataaggcag gaattacggc taataaaaat actgttccct ttgataccag
    23761 aagtcctttt gttgcctccg gaattcgttt gggaactcct gctgtaacca ctcgcggaat
    23821 gggaaaagcg gaaatggaac agattgccga tttaattaaa agtgtctatg atcatattga
    23881 taacgaagaa tacctgcaag aattgaaaaa agaagtgcat tccctgacag ataggtttcc
    23941 cctttatcct gaactgtaga cggaggacac tgttactccg caaaaaaatg aaaatgtgaa
    24001 aaggtgaaaa agtgaaatgg tggaaaggtg aaaaagtgaa actcggaatg aacattatgg
    24061 taaaaaatgc ggagcttcag cgagctccga ttacactaca attatagagt caccactgct
    24121 ttaagccagg agtcgtcacc gacgagatga ctttgacctt acttgcagac aattttggtg
    24181 gtgaggactc ctgacaattt ttagcacgca gattctttat tgattataag ataggagatg
    24241 aaaatgacta tagctaaaat ttatttaagg ataaacaaat cctgttcaac ctggttacat
    24301 tttcacctgt taatcctatg cgcattgcag caatagattg cggttccaat tccctcaaat
    24361 gccttattgc agaaaagagg ggagaggact ttaattgttt aaaagaccta agatttcaaa
    24421 cccgtcttgc ttctgctcta aataccaatg gtttcctctc tgcaacagcc atagaaaaga
    24481 caattactat ttttaaacat ttgcaggaaa atgaattccc tgcttaccaa attgaagaaa
    24541 ttcgtgctgt tggcacggag gctattcgcc aagccgctaa taaacaggaa gttgtagacc
    24601 gcatttttag ggaaacaggt atccaattat ccgttatcaa tgaagcccag gaaacatttt
    24661 tggaatggaa aggagtgctt agcggtttga aaaaaccctt acagccaata actatttttg
    24721 acagcggagg tgccagcact gaaattatta acgggaacaa aggacttatc cataaagcga
    24781 cttgttttcc tgtaggagct gtgaatttta ctcaaagata tgttcacagc gatccgattt
    24841 ccccaactga ctttgaggaa ttggttagaa agattttcca aagtttaccc caatctttta
    24901 tgatacaaga tacttttatc ggtgccggag gaggtgtata tgtatgtgcc tgtgtggctt
    24961 ccggagggaa aattaattca ccagaagaag ccgagggcta tgaaattaca ttatccgaac
    25021 tggaaagaca aattaacctt tatcaataca ccaatcttga acagcgcaaa caaattccag
    25081 gaatggaaag tgaatatgcc gatattatgc ttgctgctgt tttaatctat tggattttac
    25141 tgaaggcaag cggaaaagag aattttttgg tttctacccg tggtattaga tatggtttag
    25201 ttgcaatatg aagcacgcag attgcgctga tttattttta caccgagatc accaaaaaca
    25261 ctgagcacac cgaggtttat tgataattac aacgactttg tcaagtcctc tctgccaaca
    25321 gaattagtgt atagcgtata aactatatag tcagtgacga ctcctgactt tcataaattt
    25381 atttgaaaat ttattccggt gttctcggtg ttctgggtgt aaaaaataca atctgttgcc
    25441 attctgttac tctgttaagt gtttcttcgc tgatttccta ttaacaataa gtttgtatat
    25501 ttcaaaccag gcaacactca tgtttcctaa aatcaggcag gttaataatt ccattatact
    25561 cattgtttcc aggtgaaaaa tattagtgac agaaggcact tttatcatca gcaggacaat
    25621 ggcaaagaca cctaaaaaca aataagtcat acctttattg ggagtgcgga taatctgaaa
    25681 taaattttta tgccagcttc tgtttgtagt tatcagcatt agattggaaa acactaaggt
    25741 agtaaaaata tgagctctta aatgattttc tcccattccg cggttatttc cccaaaaata
    25801 catcagcact aataggatta atacgcctaa accttgtaga aaaccgataa ttaatctttt
    25861 agtgccaaat aatttttcat ccacagagcg cggaggtctt tccattatat catcttcttc
    25921 tttttccatt tcaaaaacaa tagaacaagc agggtctata attaattcca gaaaagcgat
    25981 atgaaccgga aaatagatga tcggataatc ggcaaatgaa gcaggaatca ttgccatccc
    26041 ggcaatggga atatgcagag aaaaaacata agtgaatgat ttacggaggt tatcatatat
    26101 ccttctaccc atcctgacag cgcttacaat ggaagaaaaa tcatcatcgg ttaaaacaat
    26161 tgctgatgct tcacgggcta catcagttcc tcttaaaccc atagaaatac ctatgttggc
    26221 agcttttaaa gcaggagcat cattcactcc gtctcctgtc atagccacga tttctccatt
    26281 tgccttaagt gcttgcacaa ttcttaattt ctgctgtgga acaacgcggg caaaaataga
    26341 gacctgttta catttttctg caagttcttc atcggtcatt tggtctaatt cactacccga
    26401 tataatatta tcaacattat tcagacctat ttgtttacct atcgcttttg ccgttccaat
    26461 gtaatctccg gtaatcatta ttaccctgat tcctgcttta taacagattt taactgcttc
    26521 cggaacagtt tctctaatag ggtctgccag tccgattaaa cccaaatagc ggaaagataa
    26581 gtcagtaagt ttttctggta aaacatcagt ttcaatttca caagaggcaa ttcctaaaac
    26641 tctgagccct tcttctgcta aatcagaaac ctgattcatt atggtattga tttcttctgc
    26701 attaacactg cagagtttaa taactgcttc gggtgaacct tttaacgcaa ctgtgtaact
    26761 tgttttgccg gaaattttcc agacctttgc catcgctcta agttcatcgg ttaaagggta
    26821 ttctctaatt tcctgataat ttttcagttc tatataagta aagggatcaa gatcattcag
    26881 tgcattatga attgcttttt ccatcgggtc aaaaggttct ctcatactgc caaggacact
    26941 atgaataaga gtattaagaa ttggctctga aagctttgcc gaattcttta tctctatatt
    27001 ttcgctgttg ctgacgattt tttgtaaggt cattttgttg taagttattg tgcctgtttt
    27061 atctgtgcat aaaactgtag ctgaacccaa actttcaatt gcctgcaacc ttctaaccag
    27121 cacatttttt ttggacatcc tccaagcacc taacgccata aaaacagtta aaactacggg
    27181 aaattcctct ggcaaaacag acattgctaa agtgatactt gtcaacaaag catttattaa
    27241 atgctttctg gtgatataaa aaacgattgc tacaacagca cagattagca acgcaaaaat
    27301 agcaaagtat tttaccaatc tgcctgtctc tttgcttaac cgggtttcct cttctgttat
    27361 ggtttccagg gattttccta tttttcccag ttcggttctt tcacctgtgg caaaaacctg
    27421 agctatacct ctgccactta caactaatgt tccgcaataa acaaagggta aatcatctcc
    27481 tccgggagtt gaaggtcttt cttttttatc ccaaatccgt tttctaaccg ggaccgattc
    27541 tcctgttaaa agtgattcat caacactaag taaatcattt tccagtatat aagcatcagc
    27601 aggaatcctg tcaccttcag ctaatataat taaatcatcg gtgacaactt ctctacctgg
    27661 aattctgatt tgctcaccat tccgaattac taaagctcgc ggactggata gattctttaa
    27721 tgcttcaagc gctttttccg ttttgcgttc ctggataagc tctatagaca ttacaatgac
    27781 aacggatgct gttaaaacaa gtgcttcctg caagtcccct aaaaagaaat aaataacagc
    27841 acaggcaact aatagaataa acattggctc acttataata tcccaaatca gcttgcctaa
    27901 attacgccct tttgaacctg gaagttcatt atacccttcc cgtcttaatt tttcttccac
    27961 ttcttttgtg gtcaatccct gttttaagtt gctatattcc ttcatcttat cctcttttac
    28021 agaatataat cttgctaact gcaatattgt ttttagtcaa gtaaaaaccg attttaagct
    28081 gagacaaata gttgaacagc agaatttttt tagcactctc acttttagat tgacaatttt
    28141 ccaaagttga ttaaattgtc tttagattag cagttcatat attggactgc taagaatata
    28201 aatggtggag acaatgaaaa agaatcagct gcgactaaat gagaccctta aggcactggt
    28261 ggatgaatat attatgagtt gtgaaccggt gtcttccagg atactgaacg agaagtatct
    28321 gcgtaatgta tcctcagcta cattgcgttt ggatttgttg aaactggaaa agatgaatct
    28381 gatttcccag ccgcatacat cggcaggaag gattcctaca ataaaaggtt acagggaata
    28441 tttaagattg attgagccag aaatagaaaa gatcagatat gaaggaatgg acttgctgag
    28501 ggagatttta gtgcataact ataaagatac gcccagagct ttgtattata taatggaaaa
    28561 tttggcgaag gagacagacc agctttcttt tgttgctgaa cctgaggttt ccagtggtta
    28621 tttggcaaag ttggatgtat tttccatagg ggaaaacaag ctgatatttg tgatgagttt
    28681 ggattcgggt ttggataaaa cagttatttt gaaatgcgat aatgagttaa ctgaagctca
    28741 actgaaaaaa ttggtgcgtt atttaaatga tgaattggct ggtttacaga tttatgatat
    28801 agctaatcgg gttttagtag aaatgcaaga aaatgcggat aatggtatga taagctggtt
    28861 cttggctgaa ttgcataaag cgtttatgga aataagcgat ttctttattc attatgatgg
    28921 cagcatcaat tttttggaac aaaaggaatt taacgataaa gagaatatat tgaagtttat
    28981 gagtttactg caacgccagg attttctctt aaatacgatg cgcaaacatc aaagcgataa
    29041 agaagttaat atagttttgg gtgaggattt agctaaaggc agctggttgg attttgctct
    29101 aatttatgcc aaatatgagg tctttggcat acctggctat ttaggtgttt tagcacctgt
    29161 cagaacagaa tatcgtaagc tcattcctct tatcagggat gtagctaata ctataacaaa
    29221 tacaacgagg cgcggaatgg tcgtgcccca ggagaaaaag gaatgaataa aaaaataccc
    29281 aaagaacctg aattagaaaa agaagaacta attacaacgg aagtttcaga agaacagaaa
    29341 gccaaaaaga tagaggaact ggaaaaagaa gtagcagaat ggaaagataa atacctacgc
    29401 agtatggctg aatttgagaa cttccgcaaa agaacaatat ccgaaaaagc ggagtggata
    29461 agattagcca cacagaaatt tgctttagaa atctgtgatg tgctggataa ttttgaaagg
    29521 gctatccagc aagcaactga agaagaaaaa tccactccct tcggaaaagg ggtgctgatg
    29581 attgaacagc aactccgcaa agccctggaa aaagaagggg taaaaagaat agaagcactg
    29641 ggagaaccct tcaatccaga gtttcatgaa gcccttgctc atattcccag tgatcaagaa
    29701 gaaaataccg ttactgccat catccaaaat ggttacataa tgaacgataa agtgttgcga
    29761 cctgctagag tggcagtttc caacggttca aaaataaata atgaattcca ggaggaataa
    29821 atgggaaaaa taataggaat tgatctcgga acaacaaact cctgtgttgc cgttgttgaa
    29881 ggcggcaaac cagtagttat aaccaatgcg gaaggaagta gaacaactcc ttcagtagtt
    29941 ggtttcacaa aggatggtca acgtctggtt ggccaattgg caaaacgcca ggcaataacc
    30001 aatccccaaa atactgtctt ttccataaaa aggtttatgg gaagaagtta tgatgaagtt
    30061 acacaagaaa ttaagcaggt gccttttaag gttgttcgtg gcttaaaaaa tcaggcggtt
    30121 gttcatattg atgtggaaaa taaggattat gctccacagg aaatttccgc tatgattcta
    30181 acaaaaatga aagaaacggc agaggcatat ttaggacaaa aagttacgga agccgtgatt
    30241 actgttcctg cttatttcaa tgacgatcaa cgtcaagcaa caaaagatgc cggacgcatt
    30301 gcaggacttg atgtaaaaag gattatcaac gaacccacag ctgctgcttt agcttatgga
    30361 atggaaaata agaaagagca gaaagtagct gtttatgatt tgggtggcgg aacctttgat
    30421 atcagcattc tggatatttc agaaggtgtt gtggaagtcc tttctactaa cggtgatacg
    30481 catttaggtg gcgatgattt tgataagcgg gtaattgatt ggattctgga acagttcaaa
    30541 aaacaggaag gaatggactt atcgcgtgac cctatggcta tgcaaaggtt aagggaagca
    30601 gctgaaaaag ccaaaattga gctttccgga acccttacta ccaatataaa cctgcctttt
    30661 attactgctg atgctacagg acctaaacat cttaatctgg atttaaccag agctgaattt
    30721 aatcgtttaa cctcagattt ggtggaaagg actttaggtc cctgtaaaag agcattagac
    30781 gatgctaaac tgaaaccttc tgatattcag gaagttcttt tggtaggtgg aagtactcgt
    30841 attcctgcag ttcaggaagc tgtggaaaag ttctttggta aaagccccaa tcgtagtttg
    30901 aatccggatg aagtagtagc tattggtgct tccattcagg gagctattct ttccggtgac
    30961 gagaaagtaa gtgatgttct gcttttggat gtaactcctc tttctttagg tattgagacc
    31021 ttgggtggag taatgaccaa acttattgag cgcaatacta ctattccgac taagaagagc
    31081 gaaattttct ctacggcagc agataatcaa accgccgtca ccattcatgt tctgcaaggt
    31141 gaacgtccta tggctgcaga taataaatcc ttaggccgct ttgacctggt gggaattcct
    31201 gctgctccaa gaggagttcc tcaaattgaa gttacctttg atattgatgc caatggtatt
    31261 ctccatgttt ctgctaaaga taaaggaact ggaaaagaac aatccatcag aattgatcgt
    31321 gctgggagca ttagtaagga agatatagac agaatgatta aagatgcaga attacattcc
    31381 gaagaagata aaaaacgcca ggaattcgtt gtcgctaaaa atgaactgga ttccttaata
    31441 ttcagcacgg aaaagagttt gaatgaatat ggagataaac tttccagcag tgaaagaagc
    31501 gaactggaag aggaactaaa aaatgccaaa gagcaaatgg aaagagcaac cgaggcatca
    31561 gaactaaaca acataaaaga aaatcttgcc aaaaaagcac aacatttagg tgaggttatc
    31621 tatgcacata tgcaacagca gcaaggtgca ggcggtgaag gatatagcca ggatgcaagc
    31681 ggttttaatt ccggagatta tgaacaggga cctcaggaag aaaaccccaa gcaggaaggt
    31741 cctatagatg ccgactttga agttgttgac gataaataaa taaagaaaac tgattaaacg
    31801 gattttagcg gattaacacg gatgaaaaat aattccgtgt taatccctct aatccataaa
    31861 tcggcaaagg aaagaaaaca ggttattata gtttatatct ttatatctgt gaaatcagtg
    31921 aaatctgtga gaggtaacat atagatggca aaacgcgatt attacgaaat tctgggtgta
    31981 gatagaaatg ccgatgaagc aaccataaaa aaggcatacc gtaaactggc aatgcagtat
    32041 catccggata aaaacccgaa taataaagaa gccgaagaga aattcaaaga agcaagtgaa
    32101 gcttatgaag ttttatcgga taaagaaaaa agacaaatat acgatcaata cggacattcc
    32161 ggattagaaa atcaatttgg aggaaccgga ttctcttggg aagaatttat gcaccgtagc
    32221 gatctgaatg atatttttgg agatggactg ggcagcattt ttgaaacaat cttcggaggt
    32281 ggttttggag gaagaaatca acgcagttca ggcaattcca atagagggga agacctacaa
    32341 attgaactat ctctgtcact tcaggaaatt gctaatggtg tagaaaagac cattaaaata
    32401 ggcattaaag aaccctgtga taaatgtgga ggaagcggaa gtgcagaagg gaaaacagag
    32461 acctgcccta actgtaaagg aacaggacaa attcgtcaaa tacggcagtc actttttgga
    32521 aggatgcaaa cggtatccga atgccctacc tgtaatggtg aaggtaggat aattaaaaat
    32581 aaatgcagta gatgttatgg tgaaggacgt gttggaactg ttaaggaaat accgattaac
    32641 attcctccag gagttgaaga tggacagtat attcggttca ggggacaagg caatgcagga
    32701 ccccgaggag gaagcagagg agatcttctt gttcttattc atcaaaaaaa ggatgatctt
    32761 tttgaacgcg aagggaataa cattattctg gaatatccca ttactgtatc acaagctgtg
    32821 ttaggtgatg aaattattgt gcctacttta accggttcag ttaaaatgaa agttccaccc
    32881 ggaactcaaa gtggcagatt attccgctta aagggacaag gaattcaagg tcataacagt
    32941 tattccagag gtgatcaatt agtgagaacg attgtagtta tccctacaaa aatctcccgg
    33001 gaagaagcag aactctataa tcgcttaaga gaatttgacc ggaaaagaga tctgaaaccg
    33061 gggaaaagct ttgctgccaa attaaagagt tacttcagct aagaaaaatg ccatctatat
    33121 atttacctga attaagctcg gaaaagaaga ttataacggt agaaggagag gaattccatc
    33181 atctgattaa tgttaagcga ataggaatcg gagaatcagt caaactgaat agtggtgccg
    33241 gtttaatggc agagggcaaa ctaattaagg ttaataaaaa atccgctgaa attgaaattg
    33301 ataaaataga aacatttcct tttccagaaa gacctttcgc cattgccttc gccttattaa
    33361 aaaaccgacg cgatgattgg ctgatagaaa agtgtacaga attaggtgtt attgcattct
    33421 atactatgtt gactgactat agtgtgcgaa caccttccca aaatactata acgcgtttta
    33481 aacggacagc tttagcagca attaaacaat gtgataatcc ttggatgccg aaaattaatg
    33541 aacctttgcc gctgaaggaa acagtaaata agatattttc cgaggggtgg attccggttt
    33601 tatgctccga acgaaaacct gataaaagaa ttacttactt accgaagatt attaaaccct
    33661 gttttctaat tggtcctgaa ggcgggttta gtgaagatga atttacttat tttagtgaac
    33721 aaaatattct ttctatatca atttgccatc taattacccg ggctgaaact gctgctatag
    33781 ctgttgctgc acaatatcaa gcttattttt aaacaaagaa aaaaaacaat ggataagtga
    33841 tagaatggta attgataact tggattttag atcatatact acaatcatct ttaccatttt
    33901 agcatctcac catttcacta ttttactatc taattatcta actatttctc tatatattac
    33961 ttaataaaat caatttgcca gagtataaac ttggtttata atattataaa aaggagtaag
    34021 atatgaataa caagaagctg aagatattga atccaattat gttcgtggta attcttattg
    34081 ctattatagc aatagttact tacaaaatct cagaagcttt taattatgat gcttatggtt
    34141 ttaaagaaat ccatgaaatt tgtggatttt tgtttgttat tttggcaatt tttcatataa
    34201 tttacaactg gcagtggata aaaagtcaga tttttaaaaa aaagccgaaa aaataatgat
    34261 aatttttgtt taatctaaat taatagtaaa cacgagaggg acatcgtttt ccggaaaatt
    34321 taggagttat caatgccatc aagatttatt tcactaccga aatatgattt agcagtgagg
    34381 attcataacg aaggtaacta tttatattta tagcatatta tagcctttat gcttcccttg
    34441 tgttaagagt gtaattggta aggacaaaat agtaaaagcg taaatggctc ctaacctaac
    34501 aattgacttt catatacgga ggaaaagtgt cccccgacta catattattg agctctatga
    34561 atttttctga tcaccgagtt taaaaccaat tattagaacc taccaaccct gctatgattt
    34621 taaatatata tggatatttt aggttatttc tctttttcct ttattttcta tataactatc
    34681 attccatctc actatctaac agacttcagt acctggttaa caaatccttc ctgattttcc
    34741 atactatatt ccagttccag agccagcaaa cggcttttca ggggctcata ttttgccaac
    34801 ttcataatat ctttttgggt gcagagaata aagtccggat ttagctccag cagtttttgc
    34861 ggcagaaaag tgttggaaaa agcgtaatgg tctttaaagg catagtgttg cataaaagat
    34921 aagcccaaag agcaaacact ctgttcaaag gaagcaggat tggcaatggc tgaggtgagg
    34981 actattcttt ttccttctat ataagagaaa ggaaaggtgt taccggcagc atctttaaaa
    35041 caggagggag agctaaaaca atgaaataca ggcattcctt tgcggataat tttttccaac
    35101 cagggaattt gtgtttgttc ttcattttta tggttaataa cagctatact ttgggaagga
    35161 atattggaaa ttggctctct taaatagccg gcaggaatca caaagccatt gccaagccca
    35221 agagaagcat caaaactaat aatatccagg tctctatgca cataatagtg ttgtaaggca
    35281 tcatccaaaa taattatctc aagatccgga tagttttgca ataagagctg aattgcttct
    35341 tttcgttttc tgccaaccgc tacaggaatt tttggcaggg aagaagctat catatatgct
    35401 tcatcacctg tgtgttgaac atcataaaga atgcctgttt tatccgcaat tagatgaggg
    35461 ttatattcaa acttgccttt atatccgcga tgggaaactg caactttttt cccttccttt
    35521 atcaaaagct ttgccagaga aatagttaga ggtgtttttc ccgaaccgcc gctaacgata
    35581 ttaccgatac taattatttt gcaaggtggc ttatattcat ttctttgata ataagttctg
    35641 cgtatttttt gtaggaaacc gtaaattaca cctgcgggat atagggtata ggatagggga
    35701 gagcgtttta gaaggtgatt ttggattaac ttgttagcat ccatttattg caagtgggat
    35761 tcaattagtt ccaccagttt ttccgtttca aagggtttat aaaggacatc cttcaaaccg
    35821 tctactttag cgcgaacgag cacatgattg gggtcataac caaaacccgt catcataatc
    35881 accggaattt tttcgttata gtccttaatt ctccaaaaca attcataacc atccatatcg
    35941 ggcatagcga tatctgtaac tacgaggtct acattgcctt ttacgatttc ctggatagct
    36001 ttcattcctt cactgaaagt aataattttc cagttcggtc ttatagcttc cagttcaatt
    36061 ttcaggtttt caaccaggtc ttcttcgtca tccacaatgc agataatttt cttcgttttc
    36121 acaattcttt accggtgata attttgagaa tagttaaata tttttgtcgg gtttgctcaa
    36181 tcacctcttc cggtaaagga ggaggtggtg attgtttatc ccaaccgcta ttttccaggt
    36241 aatctcgtat aaattgttta tcgtaacttt ttggactttt gcccatttca taatcctcca
    36301 aagaccaaag tcgggaagaa tcgggggtaa acaattcatc aatcaggaga acttccgagc
    36361 cgatagtgcc aaattcaaat ttagtatcag ccaaaataat gccttttttc agcagtaaat
    36421 cgtgagcgta agaatatagt tccagggatt tatctttgat ataaacagcg aggggttcat
    36481 ccattcggct cataaattca cggtagctga tattttcatc gtgacctttt tccgcctttg
    36541 tactgggggt aaaaagggga tgtggaaatt tttggctttc ctgcaattct tctgcaatca
    36601 gcattcctcc gatagtattg ctttttttat attccttcca ggcagaaccg ctgatgtagc
    36661 cacgcacaat acattcaaag ggaattacgc gtgttttttt taccagcata ctgcgttctt
    36721 ccaaatattc gccaaaacgc tgtaattcca cagggtattc agaaatgtca gcagtaataa
    36781 aatggttggg aacaatatct ttagtggtgt taaaaaaata aacggaaata gcatttaata
    36841 ttttcccttt atcgggaatg agggtgggga agaccacatc aaatgcggaa atgcggtctg
    36901 tgctcacaat taaaagtgtt tctcccaaat cgtatatatc gcggactttg ccttgccggc
    36961 tttgttgaac aaggggcagt tgactaattt cgttatacat taaaactcct tacatcttct
    37021 gtaaaaaatc gtagattact ttgctttcat ctacactatt cagattaaga gcaagtgtct
    37081 cgttaatacg aaactctatg ttgtcaactg gaaaataata gtccttgccc tgaaatctgc
    37141 aataggtgat tttgcctttt tgtattttca gctcgttact ttcatactcc aaatgtttag
    37201 cggaaataag gaatttcagc cattcgcggt atttgtttaa aatttctata tcctcatcca
    37261 gcagcgatga tttgtcaaat tccagttcat taaagtattt atttctttcc tgttggataa
    37321 gttccataat gctgttttca ggaaccagaa aagcttcttt taaaagggat tccaatttat
    37381 ccaggtcata atccgttggt ttttcagaag tttcatcaag agccaagcac caaccccggc
    37441 ataaattttt ttctcttttt tcacaaggat attcacctgt ctcacaggca ggtaatttta
    37501 aaatgcgcgc ataaatatca agcacatcaa ttaagaaaaa acgactgcga aaaggtccta
    37561 aataaaacca gtcatcatta gtgtgttcct gaattgtaat aaacggaaaa cgatgtgaat
    37621 ccagcgctaa ataaacatag tccgcccaag gacgaatctg atattgataa agaggagaat
    37681 tttctgttaa ataacattta taatgaatta aggcgaaaat tgcttgagga tatgtttccc
    37741 agctcaattt ttccgctaaa ttccatagtt cctggtaaat accaccctct ttggctttat
    37801 tggaaaagaa ttttagcttt gccttcaaat tggctgagta acctgcgtaa aggacttctc
    37861 ctgcaccgaa aatatgataa aaaacccagc ccttgggcag tgcatcaaat tcattttgta
    37921 tattcttatc tattttaatt tcgttcatat aacaacattt cttcagttag ggtttatgtc
    37981 aaacgaaatc ctaaataata aagaggatgc taaacttccg gaagaacgat gtccttacct
    38041 ttcacaagta ttcatttttt tgttgcggtg attatataga taaagaaata aagcgtaaga
    38101 ggaggttaac ggggatgtct atgataggac actataaccc gtaaaaaaag ttgtttaaaa
    38161 tagtaaaata ctaagagttt actttcttct ttcccatcct ccacccatcc ttaaggttgg
    38221 gttgaggatg ggttataatt aaggaaggat aaattgcagg aacaacagta aaaataccgg
    38281 ataaacgata taataaattc tggcaaaaaa gtttaaagat attgaaggag tgattaatat
    38341 ggaaaagttt ggggtttttg gcaaagatgt atgagcgaag agcgaagagt tattttagca
    38401 ttttttccga gggatttaca cacccatcgc tatcattgtg tcgccttgac gagctttaaa
    38461 gaatttcaac cctttagata ttaatacttt ttccgagcta aattcatttt acattttaca
    38521 ttctgaattc ttcattcttc acttctgtat ttgacaaatt atcttatttt ataatcccga
    38581 aaattactta taatcctgtt catcctcaat ctcttgtcta taaagaagaa atgacgattt
    38641 tccgaatgcg ttttgtggcg attactaaca ttaaagttaa gccaatcata attgtggaaa
    38701 gggaattgat agtagtgatt gttcctcttc taattgcacc ttccacataa attggtaaag
    38761 tgtcaaaccc gcgtccggaa gtgaaaaaag taataataaa atcatctatg gaaagggtaa
    38821 aagctaaaag tgccgcagca ataattcccg gcataattag aggtagtttt attttccaga
    38881 agatagttgc cggttttgct cccaaatcca ttgctgcttc caccagagaa taatcaaatc
    38941 cttccaaacg actttgaatt acaataaccg tataggaaat gcaaaaagta atatgagcaa
    39001 tcaaaacggt cagcattccc gtattaaccc tggtaaaatt aaataataaa gccagggaaa
    39061 cacccattaa aatatccgga attacgagag gaatatagat taaagcagaa aataatttgc
    39121 gtccgctgaa tttatgatat tccagtccta aagcgcctaa cacacccaaa acggtagaaa
    39181 taagcgtaga aagtcctgca atcagcattg tatttctaaa tgcttccagg attgttttat
    39241 tttgccaaag caaaacataa tacttgaaag taaacccctt ccaaccggta ataattttgg
    39301 catcgttgaa actgaagata atcaaaatta aaatgggaat ataaagaaaa accatcacca
    39361 aagtgaaaac ggtcaaattc attcctttaa aaaacttgcc taaagaaagg cgtttcatac
    39421 ctgtctcctg gatttgcttt tgcggtaaag aaagtatatt actaataaaa acaaagctac
    39481 aatcaataat agggttaatg acagagctga agccataggc cagttgcggg ggatattttt
    39541 tactttatag gtgattacct gtcctaaata gagcgatttt tgatttccta ccaactgagg
    39601 aattaaatat gaacctaaag caggaataaa aacaagcaat ccacccgcaa aaataccctc
    39661 tttggatagg ggtaaggtta tgcggaaaaa tgagttaact tcattagctc ctaaatccat
    39721 tgaagcattc agcaaagacc aatccagttt ttccaaaaca ctatatagtg gcaggatcat
    39781 atagggtaaa taaacataga ccatcactaa aataactgca aggtctgttc tcataatttc
    39841 caggggttgg gatattaatc ctaaacttaa aagaagatta ttcagcaccc cattgtaagc
    39901 aagaaaaatg cgccaggaaa agatgcgaat aatcaggttt gtccagaaag gaatgataat
    39961 aaaaatcaga agggtatttt ttaccttatc ctttgcccgt gctatgtaat aggcaactgg
    40021 aaatccaagg agcaaacata ataccgttgt taacaaggca agccgaaaag aaagataaaa
    40081 agtatgcaaa taagcaggtg taaaactctg acgatacgca tccaaagtaa attcaaactt
    40141 tacatcataa atatcggcag tgagaaaact atagatgata acaataatat agggaacaag
    40201 aaaaaagaaa attaaccata acaaagccgg cgcagttaaa atccaggaag tgaaattctt
    40261 tctgcgttct gctgcaattt gtaaagcgga attgggataa tcggaaggaa taattttcat
    40321 tggactgccg gtttttttat ggaacgctga taatgattaa ctccaccttc ggagatttct
    40381 tcaggagtta acacattttc gggaatttca ttgataagcc agctatcggt atcatgccag
    40441 aaaagccaga ccggctcttc ccaatcaagt gctgtatcat caaaataaac tcgtttatgc
    40501 tgactaaaaa ccctgatttt taaagctcca actctaacta tgtattgtgt gtgagacccg
    40561 agatataaaa tatcttctat tactccccgt aaaatattta tatcatcctg atagcgtggt
    40621 ttattacggg aaatagcgat tttttcggga cgcagggaaa cggttaattc ctgtcctata
    40681 tttggttgaa aatgaaaagt gctgtctatt tcaaaactgt ttcctgtacc atcgggacat
    40741 cttatttgca cataatcatc ttcaatgctg ataacctctc cactgattaa attggtctct
    40801 ccgataaaat atgccgagaa aacgctatta ggtcgttcgt aaatatcatc cggaggtcct
    40861 tcttgaataa tattgccata attcatcact gctacacggt ctgaaatact catcgcttca
    40921 ctctgatcat gagttacata aataaaagtg attcccacag catcatgaat attcatcagt
    40981 tcaatcaaca tatgttgacg cagttttagg tctaatgcag ccaacggttc atccaaaagc
    41041 aacaccttgg gctggttgat taaagccctg gcaatggcta cacgctgttt ttggccaccc
    41101 gaaatctgtt ccggaaattt atttgcctga tcttccattt taactagcga aagcatttcg
    41161 cgaacccggt ttttaatctc atttttggga cttttcctaa ttctcaaacc gaaagcaaca
    41221 ttatcaaaaa ccgtcatatg cggaaaaaga gaataatttt gaaaaatagt attaacctgt
    41281 cttttataag gaggtaaatc ggttatatcg gtattgccga ttaaaattct acccgtgctg
    41341 ggaatttcaa aaccggcaat cattcgtaaa agagttgttt ttccacaacc actgggaccc
    41401 aggaaggaga ataactctcc actttgaata gctaatgata cattttctac ggcaacaaaa
    41461 ccgtcaaagt ccttgcttaa gttctccaaa cggatttctt ccaactttgt ttatacctcc
    41521 tttataagct tttttagata agccgcggga aattaaaact tttttcctta acttctcctc
    41581 cttgattata ggcaaaaaaa tacagtgggg aatgccgggc ggcatcccca ctgctcggag
    41641 ggcattctat gtagggtctg taggcttgaa tcatttataa atacctacag aatcttgtca
    41701 agaaaaaagt gtagaatttt ttcattttct aagttgaaat atatcaacat agttctattt
    41761 tactattatt agcttgcttt tgtagttttt tgagccctgt aaaatttcta ttaaatacat
    41821 cccgttgggt gataaatttc ctttgttatc gcgtccattc cattttattt cctgaatacc
    41881 tttttcttcc gggaaaaatg tttgagtata aattagctga cccttcagat tgtaaacctt
    41941 gaaagtgaaa ggtatatttt gcggtgaaaa gtatttcaac tggatggcat cattaccttt
    42001 ggctttaaag ggattgggat aaactgccat aatgccttcc tgtatatttg gagattgtat
    42061 ttcatcgttc acagaaacaa ttaaaatagg cacttcatac cataaattct gtccgggaag
    42121 aaaacttata ttaggaggat tggctccatc gtaagccata aaactgtaat aaaaagtgtg
    42181 gtcattagta gtaaattcag gataatactg aaaaatataa ctatcttccg ttaacgggtc
    42241 cattgggtaa gcaaaaactt ccgaatcatc tattttgtaa cggaaaagca actgactgat
    42301 gccactctca tcgttggcag aaacaattat gcttattggc tcgctttgat tactgataga
    42361 agaaagagga ttatgagata aaaccggagg agttaaatcc ggttgaatca caaaaagatg
    42421 agggtctaag gcagcaaata ccgggtggtc aacgcttctt cctgattgat ctgccgcgtg
    42481 aataaaataa cgaatagtat ctccataagc aaatccattt aaagtgacag tataataatt
    42541 gcgagtaaga ggttgcagat aatttctttg ccaggcaccg gaatttacct tataacaaac
    42601 aaaaagagaa tcggtataaa gtggctgacc gctatgtgca attatttccg tattaataat
    42661 aatatccgtt ccaacaggga taatactatg ccagggagta tgaacaatat tcagcatatt
    42721 tttatctgga atttcatgag ttctgcaatg taaggcatca gtagattccc aaggtgcaga
    42781 actggtttga gaaacaccga taacttcata accaggcatt gcttcacgat aagcttgcag
    42841 ggcagaatta tcattactgc tgcctacaat tggcacaaaa acctttttat tcaaaataag
    42901 ggaattggtg tagggctcat tggaaggagt ataaacccga taaacacggt agggataacc
    42961 ccaggcacaa ttatgcgtag caaaataatt ggctgcattt tctatggcat tatattgaga
    43021 atggtttgca ggaacgctac gaatcattac tttatcgggt gccagaaact ttgcccaaca
    43081 gtcaatatgc tcaatataag tgttattcgg gtctgccatc accaaataat ttgtaatacc
    43141 taaatactgt tgcatttttg tgtttacatt tgtctgattg ttgccatttt cggtataaac
    43201 cagggttgtt tgagcagcag tattaattcc atcgctcata taatttcccc ctgtctgctg
    43261 taaattcatt ccataataat tcaaagcaaa atgattggca aaaacctggg gaattaaatt
    43321 gtcattagga cggggacgat tataaataaa atccactaca ccatattcac cattgccatc
    43381 aaaaatgaac cagggtccgt aatcccttgt ccagtaagaa tcagtagctg cattgagaaa
    43441 actaaccctt tccatattta ctccagcatt ggtaaaagta gaaatggcag atgattgttg
    43501 cgaactgctt acaatacaaa ttacatttgc cgtatttgcc aattgcacta ccagcgaaac
    43561 aggtatccct aaaggataac gaattaaaac atcagaggcg ggttcaaatt ctgctatagg
    43621 acgaaccgga gctacaggag gattggtttc cgtaaaacgg atttccgcat ttgctttatc
    43681 tgcgggcaaa ttatgatgcg caaaggcagt agaactcaaa atacagagca cactgcacaa
    43741 taataatatg cgtaaaatac gctgtttttt catcagcgtt cactccttat tatattatac
    43801 caaattataa ataaaacact ttttataccg aaattctata tttttctctc ctgcccttat
    43861 cgcagaatta attcaccttt gctatgatgt aagaattgca ggttaactat agcataatcc
    43921 attttttcta tttagcaaat tcccttggca acgaataatt cccagggttt tttcaagtta
    43981 tacaccataa cttccctttt tttaccgcct aaattatcgg gaacgatttt ttcatacttg
    44041 tttaatacca gataggtctt aaaaggaagt cctgataatt ctgccgcttt ctccactatt
    44101 ttgattccct ttagcacgat ttccaccgtt gtttcatcca tcaggttggt gttacatata
    44161 atttctgtaa tttttaagcg ggaagtggct tccaatgcct ctttcattga tattatctct
    44221 tccggagttg aagtaaaagg acgcatagtg tttataatat ataacatatt ataaccccgt
    44281 gtttttatgg gttcttcaaa acgccctaaa gttctgcaac cggcagggtc accacctaca
    44341 tccagaataa ctgttttcgc tttattttga atagctcctt taacacgggg agaaatcatc
    44401 ggtaaatcgc tatgcttata ttcaccttcc ggagcaataa tatcaattcc ttcctgaata
    44461 aaatcatcct gcatttcccg tgacctgaaa taaggattga ccacatccaa atcagctaaa
    44521 acaacatcca aaccttgtgt cttaaaacga cgcgcaagat gaatggaata ttcacttttt
    44581 ccgcttccgt aagcaccgat gatgatatat aagtgtgagg gcataattca aatccttaca
    44641 tttaattgaa acgggagatt aattcttgag aacggtctaa attcaaagac atcaaagttt
    44701 atttccaggt gcgaaacagg tcttctgcca gggacttaaa atcccaataa aagccatcaa
    44761 ctatctcttt tatcagggaa tgctgatcag agctatcttc atcataaatg gcaaaaacgg
    44821 tcatcccggc ggattttcct gcttgcacac ccgttaatgt atcttcaatt accaggcaat
    44881 tttccggttt ttcctccaag cgttccgcag ctaaaagata aatatccgga tagggcttcc
    44941 cttttaaatt tatatctccg gtaacagcac actgaaaata ttgccacaca gaattccgga
    45001 taagcacttt tttagccagt tcaagagaat tactcgttcc cagtcctatt tttatccctt
    45061 tttcttgcag acaggaaagc agttctacag cacctggttt cagcataata tccgtttcat
    45121 aatgcttgct gaccattttt gtccattcct gcataatact ttcaggacta tcgggcaaac
    45181 caaatctatc cttaaagtac tgtgcggttt gaatgaaact gtttccctga ggaagctctg
    45241 aaaaaagatc gggaggaact ataagaccgc gggtatgtaa aaattccctg tccacattac
    45301 gccagagctg cattgaatca attaaagttc catccatatc aaagattact gcctgaaaat
    45361 tgttcatcta cagaaacatc ctttattgta taacggaata ataaaactgg agcgggaaac
    45421 gggatttgaa cccgcgacat caaccttggc aaggttgcgc tctgccaact gagctattcc
    45481 cgcttggtac cagaggccgg acttgaaccg gcacgagctt tgggctcgag ggattttaag
    45541 tcccttgtgt ctacctattc caccactccg gcaaaataat aaactggagg cgacacccgg
    45601 atttgaaccg gggataaagg ttttgcagac ctctgcctta ccacttggct atgtcgccta
    45661 aaaaattaac tggagcggga aacggggttt gaacccgcga cttcaacctt ggcaaggttg
    45721 cgctctgcca actgagctat tcccgcaaaa taatcttttc agcagtcacc aaaaaataaa
    45781 tgaccgcttt tctgtcaatc aaaatgtatc tgcttcgcta aagacatcag cagtaaaacg
    45841 atatacaacg ctggcaggat caagatatgc attgggaggc aatcccgctt tacgacagac
    45901 ctcacgcaga aaggtctccg gttgccaatt gtattcaaca gccacctggg gcaaaagtaa
    45961 accgctacca taaggatgct gaatgtaaag tccgtctttg ccaattaccg gtaactcgtt
    46021 tttttgcata ggaataagtt cactcaaaat agatatttca atagttactt tttccaattc
    46081 cttttctgtt aaaggaggga aacgcgggtc ttgaaaagca gctgcttctg ccatttcttt
    46141 cacactttca gcaatggact tataaccctt tatgtaaccg atacagccac gtaaattatc
    46201 tccaatatgt aaacttacaa acacaccgcg tttaggactg aaggcaggat cctcaggcaa
    46261 agaaacggac tgatgtttaa aacgagcact aattgcattt cgggcaaggg aaagtagttc
    46321 ctttttctgt tccaaagtta acatttatcc tccttccaga tattaaatat aaagtttagc
    46381 agaaagataa cctactacct gagaatcgtt tccggatatc tttcccgaat gggtatattg
    46441 aattactttc attttggcat tagtgtaatg ctgtgcataa tacaacaggc agaggattcc
    46501 cccgatgccg catgcctcac aatcgctatg aataatatcc tgccaaagat gttccgggtc
    46561 taaagcagta acatctttta ctaaaatacc atccatttct tcggcagttc gggcacgatg
    46621 gtaatgtgat aaatccgaag aacagaggat aacgatacgc tttgtagaac tgtaaatcgt
    46681 ttcataaagt atatctgcca ggcgttttgc cactgcagga atttgattac caaacataat
    46741 aggcaagatt tttgccttgg ggaaaaagta atttaatatg ggcagttgaa tttccataga
    46801 atgctcttcc tgatgtaaaa tgagggggat attttgatct gcttcaggag ctattttgtt
    46861 gtaaagctcc atatccaaat ccaaagtgcc taaaggattt acatattcct gatagggaga
    46921 aacgctgaaa tcaaaaaagt ttgcctgatg gctgggatga agaattatga aactatcaat
    46981 attttcgtgg gaaatactat gtaaacccaa agtagcacat tcaccggaat acatataacc
    47041 agcatgagga agaataactc ctaaagctct ttctgaagaa agaggagtta cagcattgct
    47101 tatccaaccc tctatctgtc gcttgatctg ttgttcaaac cggggataga aggttccagc
    47161 atgcatagga ttgcgaatca ttgttcacct ccttgaagag gaagatatat tatataagtt
    47221 cctgattttt tctcttccgc agttaaattt gctgtctgcc aattttcgct ataggaaggg
    47281 ctaataactg caattttttt attaggtaga attgactgaa ccctactgac agttccggaa
    47341 aaattacggc tatgaaaatc accgttaaaa tggagaaccc gggctttggg tttcattttt
    47401 agggcattca caatgctttc tgccatagtt tcatctttca aacattgagc ttgataaagc
    47461 caatccgggt tattattcat catcggagaa tgcatatctt ccagagttgc caggaatgcc
    47521 tttttatatc cgtcatcagg ataatctatt ttatcgggca accatttttt atcttcttct
    47581 gccaatgttt cagtaaagtc aggtcctgtt cttgccattt taccagcgat agaacgagga
    47641 atattagcag ctattacagt cagttttttc tgtttggcat attcaatcag gggacgatag
    47701 tcatcttgat aattactcca gggacgactt ttagccaaaa attcatcctc actaagccaa
    47761 ccttctatat aagcatccaa atcactttgg acatcgcgtt caaacatttc cagagagaga
    47821 atcagttctc tttttgtatc cagcagagga acaagttctc tttccagctg atggagagta
    47881 gcattatcgt gaaattctcc gaaaaatata atatcgtatt tttccaatcc tttagccatt
    47941 tgttgcaatg taagagtttt actattgcgg gaatccacaa tacgatattc atctgcattt
    48001 aatagagtta agttcattat tatgatcata attactatga tatatttcat tctcacttcc
    48061 ttaatagttc tactgataat ttaagcgaat tttgtgttta ttgccatcaa taattttacg
    48121 aaattccttt tcttgttcag ctggaagatt tagtataaag ctaacttctt cagaccatat
    48181 cacttgcttt atttctcctc cgattgccag aaaagaatgt tttagacatt caaaagaagc
    48241 ataatcacaa acaagaggaa aagaaattag caactttgct tctattagat tacttttcag
    48301 gattgcttgt tcaactgctt cagaataagc agcaatcagt ccttttacac ctaattttat
    48361 acctccaaaa taacgggaaa caacagccag aatatttgtc aggtcatttc gcaaaagtgt
    48421 attcaatatt ggttttccag ctgttccagt tggttctcca gcatctgaat aatagataat
    48481 ttctctgttt aaaccgtgaa tataggcata acaattatga gtggcatcag aaaaaagccg
    48541 ataatgctca ttcaaaatat ctttaacttc ttctacatag ggaagaggat acaacactgc
    48601 cacaaatctg gagcgcttta tttttatttc actatcggtc tttttttcaa ttgtatacag
    48661 cattttttaa ctcccgcact ttgccctcct gcacttcagc actttgtact cccgcacttt
    48721 atttcacctt cggaaggtca ttccagtggg ttgtataatg aggtgaaagt tttgcccgtt
    48781 tcattttcca gtcctttttt attccgctac tggcataaaa aagagtgtcc tgaccataag
    48841 ttctatttag tttatccatt gcttccatca gttttttgcg tttatcatcc agataatttg
    48901 tgtccataaa gcttaaggga acatcttttt cgggaataat atcagccaac attactccgg
    48961 cttttttaaa ttcaaaaccc ggtaaatata agtcatccag caggttaaga gctgttttta
    49021 tcagttcggg agtataagca gtagggggaa agagagttgt agaaagagaa ttgttgtatt
    49081 gaggtccttc cttaaaacgg tttgtagaaa gaaacaccat cagcaatcca gccacacttt
    49141 tttgtttccg caatttttct gcagcccgag taacatattc agaaattgcc tcttgcaagt
    49201 cattaagaga ggaaacctgt ttaccaaaag agcgggaagt gacaatactt tttttgtttg
    49261 caggagcttc atccaaatca atgcaagcat agccacgcag ttccaaaacg gtttttaagc
    49321 ccatacttgt taaataatgc tctataaatt tatcctctgc attcctcaat tggagcgctg
    49381 tttcaatttt atgctgtctt aaaaatttat catattgcct tccaattccc caaacttcag
    49441 agacaggtgt ttttgccagt gctttggcaa tgcgttcatc atccaacaaa caaagcgaac
    49501 cttgaaaagc agaatagcgt ttggcaaaat gattggcaac tttagccagc gttttggttt
    49561 gggaaattcc caccgaaaca ggaattcccg tccactgaaa aaccgttctt tttatctttt
    49621 tgccaaagtt ctccaggtct ctatttttaa agccgtttaa ccctaaaaat gcctcatcaa
    49681 tactgtatat ttctacttcc ggagaaaaca gagaaagaac ttccatcact cttgagctca
    49741 tatccgcgta aagagcataa ttggaagaaa gtaatgttcc accgtgtttt ttaatcaatc
    49801 cctcatattt aaatccgggt gctcccatag gaatttttag gtctttaatt tcttgtgaac
    49861 gggaaacgat gcaaccgtca ttattagata aaacagctac cggtttatta ttcaatttgg
    49921 gattgaaaac tcgttcacag gatacataaa aattgttgca atccaccaga gcaacaatgt
    49981 tatcattttt taagccggta gtattcattg ttttcctttt tcttctgttc tctttttctg
    50041 tgtgttacct tattcactat tgcctattat tgtcaagaaa agtcagtaag gcaagaggga
    50101 gattttgttt cccgcagatt acgcaaattg cacgcagatt tcaccaataa aaatattgca
    50161 cacgaatttg acgaatttta gaggatttta atggataagg gcgggagaat ggaaatgaag
    50221 aatataaaat tcagaatgta aaatgaatta tgctcggtaa aagggttaat atctaaaag
//
LOCUS       DAOOZV010000007        48419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_851_length_48419_cov_63.507278, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000007 DAOOZV010000000
VERSION     DAOOZV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 48419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48419
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_851_length_48419_cov_63.507278"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1478)
                     /locus_tag="PLW08_01545"
     CDS             complement(402..1478)
                     /locus_tag="PLW08_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HPL51321.1"
                     /translation="MDFKDEIKQIADKIEMQKDQIQTEEATKNAFILPFIKALGYDIF
                     NPFEVIPEYVCDIGTKKGEKIDYAILKENKPIILIECKHWKSELNLYESQLLRYFHVS
                     KAKFGILTSGIIYRFYTDLKEPNKMDEKPFFEINLTDIRENQIEELKKFHKSYFDIDN
                     ILSTASELQYTNELKNMIHNEIINPSEPMVRLLAKNIYSKPLTSKIIEQFTELVKKSV
                     NSVISDIITERLKTVLIKEKEAETDKKKEEKDNIIKDDGINTTMEEIEAFYIVKAIVS
                     KRVALSRITYKDTKSYFVIMLDDKIQKPICRLEIGKNTIILTTFDEDKYRKRHKLNSL
                     EQIYDYSKRLLDTVDKYESSNNKK"
     gene            complement(1884..2864)
                     /locus_tag="PLW08_01550"
     CDS             complement(1884..2864)
                     /locus_tag="PLW08_01550"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPL51322.1"
                     /translation="MLSIFSPSNYAPFNIACEEYILKNFPEDVFLLYINNPSIIVGKH
                     QNTLAEINYEWVREHNIPVVRRLTGGGSVFHDPGNLNYSFLMNESEDFNRNFERYTKP
                     ILAVLQELGIPAVLEGRNDLTINGCKFSGNAKTNAFGKTLQHGTILFSSNISDLTAAL
                     KPREEKFNDKAVKSVQARVTNVSDHLPYPLSLQDFVTLVRAKVRTMYPDIQDYTFSLR
                     DKEEIQALMNSKYDTWEWNFGKSPRYNLCHSLKTKVGIIEFYLLVNKGIIEEVNIYGD
                     FFTNREISELENALCGIEHKPEKVAKVLKDMEYKSFFGEVKLEEIVKAMF"
     gene            complement(2960..3370)
                     /locus_tag="PLW08_01555"
     CDS             complement(2960..3370)
                     /locus_tag="PLW08_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPL51323.1"
                     /translation="MDIKEILSQIRSIANNALGGGYSFGQPSKLGDLYVIPVARVTYG
                     MGGGADESSPDTAKKEEEDSSSPAEEKGKKVKIKVGFSGGGGIGIHSVPVGLFSLKEE
                     RIKFHPVISFWEIIAMMSLSALFVLKMKKLRYKK"
     gene            complement(3513..5360)
                     /locus_tag="PLW08_01560"
     CDS             complement(3513..5360)
                     /locus_tag="PLW08_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51324.1"
                     /translation="MMRYKVLILAVLLLSSAFLNSGISRYYTFAYTTAEYNPINGIQL
                     TFPDMNNYISSGIDIGFSFPYCGQIYEDVKISTNGFINPGANWNDDSPNNQLHYGIYP
                     IIAPLWDDLSLAEGSLQYATIGTAPNREFWVQYSNARWLYNNFNCYVNFQVVLSENGK
                     IEFRYGNTNGSPLNCSASIGINMPNAGAGNFFSITPGNPPTVSTTQDNYTINEIIPAG
                     TVYIFNPLPPVLYDLSLKIEYSMFYTEGIYHNLHESFPLPVTVKNNGTETVDSYDVVL
                     YCGLTELCRLNGISLAPADSHLYDLVATIDSSGWKHLTAIAELPEDEYPEDNIGDYTC
                     IIRPNPNTGIIVGSGDELQRIPIDVYWKYSLYETIYYAEEIGNSGTIYGISYYMNSTF
                     SHNLLVKIWVGATNLTNLNEGWIAAGELTPVFNSYIIMPPESNEVYFPFSTPYQYQQG
                     NLCVMVYHSHTYALACTDPFLAQTVPEMRALKRWTDAYDPDPYNPPVYNELNGKMPKT
                     AFYLIPDIPVQDEYLPQAKIIGCYPNPFKETTTLQLNLDKKQEVCLEIYNIKGQKVQT
                     LFNGLAEKGETEISWNGLDNNNRTVANGLYFCKLKTKDRCETAKLIVLK"
     gene            complement(5716..6297)
                     /gene="nadD"
                     /locus_tag="PLW08_01565"
     CDS             complement(5716..6297)
                     /gene="nadD"
                     /locus_tag="PLW08_01565"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPL51325.1"
                     /translation="MQGKVAVLGGSFDPVHSGHLHIANQILQQKAAETVLFVPSGHHH
                     FKKNSIILPFEKRYALVKKAIKNNPQFAISDADQEGSGYTAHLMQKLKRRYPDVDFSF
                     VIGSDNLKELHLWYDYPYLAKELHFLILPRPGYALLPEVISQLKATVLNIELCPISAT
                     EIRQRIKNGESIKDMVPEELEQEIIQLYRETGI"
     gene            complement(6299..7033)
                     /gene="bamD"
                     /locus_tag="PLW08_01570"
     CDS             complement(6299..7033)
                     /gene="bamD"
                     /locus_tag="PLW08_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPL51326.1"
                     /translation="MRKYIFLLLITVFCLVSCSSNKTQLSTEAKLKNADELFAKKKYS
                     RAAVIYEEISFERKSAATAYATMKVADCYFNMNKFSDARAKYQQFINSFPDHENVADA
                     YFRIGVCLFEESLPPQYDQTETIKCIEAFQTFIDRYPNDPRYVQAVDYIHKCQYKLLE
                     KQYLTGYIYYKMKDYSSALMYFDEIVSLGNNDELDRQSLYYSAKLHLHQKNYDKAKAS
                     YERLKNRYPDSKEAKSLAKKFAKLEK"
     gene            complement(7048..7623)
                     /locus_tag="PLW08_01575"
     CDS             complement(7048..7623)
                     /locus_tag="PLW08_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51327.1"
                     /translation="MKKYLLPVCFLLLISKLLSFQETLTLDQIITEIATEIQTGQPIF
                     LDINCGEWNPVLTQKLSTNLLEKGADLRYNLSETNAGISSPEEKKVPDLSALGLEKAL
                     LVQINLNIAWQETVKSSFFSYRTERYPVHIFEIRQIQLPTQQILKISTHNLPRPNPDV
                     SGTSSFHFRWFEPLVAGAAIGSLIFLLWNFD"
     gene            complement(7627..9033)
                     /locus_tag="PLW08_01580"
     CDS             complement(7627..9033)
                     /locus_tag="PLW08_01580"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPL51328.1"
                     /translation="MKKTKQEKLTYEPRNFWKEATPEEQKKAMAYAIPYIEFLNKAKT
                     ERETIAYTLEILKNNKFSPIGKKGSRKVYSVFRNKTMAMAVIGSEPISKGFNMIAAHI
                     DSPRVDLKQNPLYEDNNSALSCMRTHYYGGIKKYQWVSTPLALHGVIIKKDGSVLNIC
                     LGEKEDEPVFVIPDLLPHLAGKEQYTKKLDEAIEAGKMNLVFSGMQEPGNEEKEAIKN
                     YALRQLHEKYGITEADFQSAELQLVPAFKARHSGFDNSFVIGYGQDDRICAYNALMAL
                     INNLPDKPKRTMVVYFSDKEEVGSQGNTGAQSIFIQDFISDLLAHNNEDNSSANLRKT
                     FINSQILSADVTAAIDPNYPNVHEKQNAVIFNYGIGISKFTGRGGKYSCNDANAEFIA
                     KVIRIFNQAGVFWQSGELGKVDEGGGGTIAYFLSNLGAEVLDCGVGLMGMHSLYELCS
                     KADLYSTYLGYKAFLQAD"
     gene            complement(9049..9528)
                     /gene="greA"
                     /locus_tag="PLW08_01585"
     CDS             complement(9049..9528)
                     /gene="greA"
                     /locus_tag="PLW08_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPL51329.1"
                     /translation="MDLSQFITKTGMQRLQKRINELLAERPEVIKAVAIAREFGDLSE
                     NAEYKAARERQRAIDNEIDYLRRRSANLKVIDTSEFPKDMVRFGTYCVAEDINTKECI
                     CYHLVGPEELNYYNEEGIQEVSIVSPIGKALLSKKIGEIAIVKAPIGERKLKIIEIK"
     gene            complement(9532..10344)
                     /locus_tag="PLW08_01590"
     CDS             complement(9532..10344)
                     /locus_tag="PLW08_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51330.1"
                     /translation="MLTIDWKERLNMDTADFLKNKLPKGDYDFEIIFIAYPERVNGKI
                     PAEVITHVASNIVQQLGKKHDAYLPFYRALWNKKGDYGKLAFGQILSKLLNRKPSVYL
                     PLLEEALSNATMAEINALLDKVMLPLLRKYPEKYLNKVFQYSNSSNPALQKSALNLLI
                     KLVKRREDLIPQIMTFFSRQWLSPLGEAVPYHITMLKTVAKLNPEYYLKIWEEFSFSH
                     DPQIVELLCTSITDYYPELESPVEIWTKSGNARLKKAATTAQRTLKKKKGAQ"
     gene            complement(10508..10581)
                     /locus_tag="PLW08_01595"
     tRNA            complement(10508..10581)
                     /locus_tag="PLW08_01595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10547..10549),aa:Gln,seq:ctg)
     gene            complement(10591..10665)
                     /locus_tag="PLW08_01600"
     tRNA            complement(10591..10665)
                     /locus_tag="PLW08_01600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10631..10633),aa:Gln,seq:ttg)
     gene            complement(10683..10774)
                     /locus_tag="PLW08_01605"
     tRNA            complement(10683..10774)
                     /locus_tag="PLW08_01605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10737..10739),aa:Ser,seq:gct)
     gene            complement(10800..10885)
                     /locus_tag="PLW08_01610"
     tRNA            complement(10800..10885)
                     /locus_tag="PLW08_01610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10849..10851),aa:Ser,seq:tga)
     gene            11087..12430
                     /gene="hflX"
                     /locus_tag="PLW08_01615"
     CDS             11087..12430
                     /gene="hflX"
                     /locus_tag="PLW08_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPL51331.1"
                     /translation="MNNEEYKALDEFELEPENWEQAERTEKTAFLAALANSRETDKDI
                     KESLNELERLAETAGIQVLGTYHQRRNAPERGTYFGKGFLTDLSRKMMQAQADILIVN
                     EELGPSQAYNIERDYQIKVMDRTEVILDIFHKHAKTREAKLQVHLAELEYQLPRLKRM
                     WEHFDKERGGVRNTGGTATRGMGEKQIEIDRRLIKDKIRKINKAIETIQHQKETQRKQ
                     REKAKKICLVGYTNAGKSTLFNQLTNAGVLVEDKLFATLDSTSRQLKLSTGNPVVLSD
                     TVGFISKLPHHLIASFKATLMEVQDANLLLHIVDVSDERFEYYIQQVNSVLQQIGAET
                     IPQILVFNKIDNVDSILVSLLESRFPEGVFISAIQGINIDKLLAKMEEILLGNRILQL
                     KIPYEKTALVSKMHDIAEIISEDYKEDGIYLKVEISRDDRFLVEDYVLENEEGKN"
     gene            13132..13527
                     /locus_tag="PLW08_01620"
     CDS             13132..13527
                     /locus_tag="PLW08_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51332.1"
                     /translation="MLIRKIRYIHIDNRSSRVISASKVISEAEILKALMTPDLNFYGG
                     FTRISTFRKSKPSTSLCRPDGLLKKGKEKRKNSGLLSSEGLTPPSLLLFRPSGFLAKS
                     EELRAESVFPFFNEGLTPPSLSLFRPSGF"
     gene            complement(13586..14239)
                     /locus_tag="PLW08_01625"
     CDS             complement(13586..14239)
                     /locus_tag="PLW08_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPL51333.1"
                     /translation="MALDKRAMIGARIAKEFKDGDYVNLGIGLPTEAANHIPEGISVI
                     FQSENGLLGAGPKPPEGQEDKDMINAGGGYITCIPGASFFDSATSFAIIRGGHIDATV
                     LGALQVDQEGNLANWLIPGKMVPGMGGAMDLVTGAKKVIVAMEHCDKYGNSKILKKCT
                     LPLTAKGKVNLIITDMAVIEVCPEGLYLREIAEGLTIDDVVQATEAELIIPERVNTF"
     gene            complement(14241..14897)
                     /locus_tag="PLW08_01630"
     CDS             complement(14241..14897)
                     /locus_tag="PLW08_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HPL51334.1"
                     /translation="MVQIISAAEAAAMIKPGNRLAIGGFLAVGAPEKIIDAMVENGNK
                     DLHIIVIASDWEDRGVGKLVVNKMVKSAQVSHMGTNKTIQAQMNAGEIDIELVPQGTL
                     MERVRAFGAGLGGILTPTGLGTIVAEGKQIITVEGKDYLLETAIPSDFALIKAHKADK
                     LGNLTYRKTARNSNPIMAMAGKITIAEVDEIVEIGELDPEEVITPGVFVNYLVLAKEN
                     "
     gene            complement(14928..16010)
                     /locus_tag="PLW08_01635"
     CDS             complement(14928..16010)
                     /locus_tag="PLW08_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL51335.1"
                     /translation="MDNLKKQILIVDDDLAILDCIRMSLQKDFPCRITTCSDSREALE
                     LLESNIFNVIISDIGMPNLSGFQLLEYINKLNPNIPVILITGEVDTEKIRSAVQLGAF
                     DILHKPFELSDLHISVKQALQKNSLLLQNEMYRLNLEELVEKRTKELYIAQCQLEKNY
                     LNTIHAMVNAMEANDVYIRGHSERVTVLSIMLGKIIGLDAEDLKLLRIGAILHDLGKI
                     GIYDTLLNKKGSLTSSEYEMVKQHPVIGAKIIRPIGLPTPVYEIILQHHEWFNGKGYP
                     YGLTGNQISPLARIVTIADAYDAMTSKRAYRENLDAKSARQEVINNAGIQFDPEYGKV
                     FFDNYSHIVEPVSDSPAIQNLLFDML"
     gene            complement(16045..16716)
                     /locus_tag="PLW08_01640"
     CDS             complement(16045..16716)
                     /locus_tag="PLW08_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPL51336.1"
                     /translation="MCGRFAQVIKYQDLKKMTDELKIKESSEQLELNYNVAPTNIVAA
                     VVAKDDWRYLGFFRWGLIPSWSKKIPEQALINVRSESILEKPSFKTSFLRRRCLIPAN
                     GFYEWRKTDKQPFFIKAKGDNLLYLAGIYDAWYGPDGSYIPSLGIITTSANDFIQPLH
                     ERMPLLLNPSLYDTWLNPAAQNPQELQLLLTVPSEIELEMYPVSRRVNKPENNDADCL
                     KPIAL"
     gene            16818..17462
                     /gene="nth"
                     /locus_tag="PLW08_01645"
     CDS             16818..17462
                     /gene="nth"
                     /locus_tag="PLW08_01645"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPL51337.1"
                     /translation="MQNRDIDTVMERLKKHFYSVKTPVVDLIQIKTEDPFKVLVATIL
                     SARTKDETTAKVAEKLFPKVQKIEDLEKIPLAELDTLITPVGFHRVKAKHLKELPKVL
                     KEKFNGKIPEEIDDLLELPGVGRKTANLVRAVAFQKPAICVDVHVHRICNRWGYIQTK
                     TPLETEMTLRQKLPEKYWLNFNSYLVAFGQNLCTPRKPKCEICPVAEFCKRVGV"
     gene            complement(17626..18069)
                     /locus_tag="PLW08_01650"
     CDS             complement(17626..18069)
                     /locus_tag="PLW08_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPL51338.1"
                     /translation="MQLLPWQIWMILGIGFIIIEIFDPAFFFISLGIGAIITSLLSLI
                     PFLGNSVPLQILIFAVLSFISFLFMRKLGKKLLSKPGGNTNVYALKGQNGFVTKEIPA
                     EGKGYVKIGGEEWPAIEVENKAVELGAKVVVEGIEGNKVIVRKIP"
     gene            complement(18070..18990)
                     /locus_tag="PLW08_01655"
     CDS             complement(18070..18990)
                     /locus_tag="PLW08_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51339.1"
                     /translation="MKKREKRRVRGIAIFIVFLAIAASIVQWQNKAGKHETYNFKPAQ
                     NEYRDLKFLNKAQISFSSPNVAKAKTEIEKIISEHSTKQIRKQSEGSFGAYIFSVPQK
                     DLPIIVEKLRSFGSVNSQTEQIDTALVNIDYESELVRLQSYEREQAELNNVRFPTDTQ
                     NRRKEELHSLIQSTRSNLDKLKESNNVLLFITLVPQQKTNTIGVTIKSLALNFFSWLI
                     IFAVGAILAYYGTRLLMYFLAALGVKGIGAGGLGGSYRYGGYGGYYNRYYNRYGYGHG
                     KRKVKRIYKDKRTTPVSEDEDTEQSEEGKQ"
     gene            complement(18992..20695)
                     /gene="recN"
                     /locus_tag="PLW08_01660"
     CDS             complement(18992..20695)
                     /gene="recN"
                     /locus_tag="PLW08_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPL51340.1"
                     /translation="MLSELYIKNYLLVPEMRLSLDKGLTVISGETGAGKSIVAGSISL
                     IFGDNLAGIEAFDKDQPIYLEVSYNLEEAEELNNFLAQNGIETEEELILAREISPNGK
                     SSYYVNGRKVGVSLLKELKSYLIDFHHQRDQQRLLSPSYQLDLLDAYADLTSIREEFA
                     NLYRELKAGLKKLDELKNEAERQKEINELYRFQYEELENAKLHINEDVELQNEYELLS
                     HTLEITELYNQITAELLEQENSVFDIISGYLSKLKHYEHLNPEIGYAVNNMQQALENL
                     QEASLNLSRSKDNVSFEPQHLQDIQARLDLINSLVHKHKVRSIEELLNLFAERSAQIA
                     AFADNEKSIAELEKHLEKNFAGLREKGEELSRKRQKSAVELSKELQENIRALSIPEGI
                     LEIMIDKKAEDNFVISDYLSAVSESGQDSVEFLFSANPGFELKPLSAVVSGGELSRIL
                     LAIKKVLADRLEPKLIILDEIESGIGGKTAAIVADFIAKLAERQQVLCITHLAQIAGR
                     AEKHLVIEKFTQGEKSFVNIRKVEAEQRLQEIARMLSGTLTAKALEHAAEILKDINIR
                     G"
     gene            complement(20695..21549)
                     /locus_tag="PLW08_01665"
     CDS             complement(20695..21549)
                     /locus_tag="PLW08_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPL51341.1"
                     /translation="MQNFAVYINPEFSDKKSIYNLLEKLRNDSDINFFSIESIPDLPK
                     ELFKPLPKPANSKHIDCILVFGGDGTILKAKDLALLTGAPILGINLGYLGFLSESVLP
                     EIASSIENLKQGKYRLLHRMLIECHLKREGKIIYEALALNDAVIHKAESPGLIHIRIK
                     ASGRYVFDTRCDGVITATPTGSTAYSLAAGGPILAPEMKAIVLAPLNPHILAIRPMVF
                     PATERLAMKVYGLSQPAMLQIDGQNSQTIQEGDEVFVTASERSVSFIKLSNRTFYQIL
                     RRKLNLGK"
     gene            complement(21549..22613)
                     /gene="rsgA"
                     /locus_tag="PLW08_01670"
     CDS             complement(21549..22613)
                     /gene="rsgA"
                     /locus_tag="PLW08_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPL51342.1"
                     /translation="MKQKDKKKTKRWTGKIKNVHLPDIEILDEIKEKAEFKASRISTK
                     ANQSYKEGLKLVQGRIMEIKSNYRYIVEINGELVTAKMSGRLKQFLYQSHTIAAVGDF
                     VAVDVANNSDRRIEKILPRRSALIRYGTGSFQKEIILAANIDQVIITSSWRKPRFKAG
                     LIDRYLCIVAIQKLKPIIVINKIDLLEDEQELKEQTAYYEAMGYPLICTSAVNGTGMQ
                     ELKELLKNKDSVFTGHSGTGKTSLINYLEPSLNLPTAEVSDRNEKGKHTTSQSILIPW
                     SFGGHLLDTPGIKTITLHSEDKEHIPKVFPGFERLYSKCKFSDCRHINEDGCAVLEAM
                     EEGIIPYERYDSYQYLYASL"
     gene            complement(22637..25558)
                     /locus_tag="PLW08_01675"
     CDS             complement(22637..25558)
                     /locus_tag="PLW08_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPL51343.1"
                     /translation="MKQKKLLYGFQLIDKKEIKEIKVTAYRYQHLQSGAELMHYECDD
                     DNKVFMIGFKTVPEDNTGCPHIMEHSVLNGSKNFPAKSTFMELVKGSMNTFVNALTFT
                     DMTCYPVASTNNKDFINLMRVYLDAVFFPNIYNEPNILHQEGWHLELTSEDAPLKYKG
                     VVYNEMKGALSSPENSINRKSRQAQFPDTPYGFESGGDPEAIPELTYEKFIAFHQKYY
                     HPANSKITLYGDMDIEETLKIIDEEYLSQFEDNNEKVQIPLQKPFGKTKVLKMEIPVE
                     EGKDIEDQYYLALNWTYGQITDKYLSQTLKALAEMLMDTPASPLKKAIQDSGYAKDST
                     IVVEDDILQPTVFLLCKQVKKENIEPLTKLIKQELKRIVKEGLDKKLIEAVINKMEFN
                     LREAQYRFYPRGLIYALNSQGLWMHNGNPVDKLAFEPMLKELRKGLKEPYFEELIEKA
                     ILNNKHSSQITFVPVPGLIQKMEQETAEKLAALKKKMTKKEIAKLIEFNKKLIQWQQE
                     PETKENLEKIPMLSLSDLNPEAKHYPTEEDIWNDIKLLKHPANTNGIVYFKSYFDLAH
                     AEEEDLPWIKLYTQLVEWMNSENYSYAKRSTEIDSNTGGIYLDIALYNSYQTPDDILP
                     KILLRGKAVKNKFGKLMELASDFALKPLFDEPERLKKLLAELKAKSEAMLPFKGHTIA
                     IQRMLKPLSQIYHWIDITNGLGYYHFLCDLVSSMDSAIDEIMEELNWIKKTFFTTHNM
                     IISITADEDIIPSAIEKLGTFADNVSTEAFAPVESHFAVRQFNEGIYAPVQVQFCAKG
                     GNFFRKGFSYSGKLRVLNNILRSSYLYQELRVKGGAYGNMSDFTLGGYLYFVSYRDPN
                     LRETLEVYNTIPEFLRNFDCDKREFDKYIIGEISSLDFPLTPEGIGDKADEDYITGFT
                     FEDRQQIRDEVLSAKIEDMRNYAELIEAVMSKNHYAVFGSESKVKEAADLFDAITPAI
                     R"
     gene            complement(25569..26444)
                     /gene="dapA"
                     /locus_tag="PLW08_01680"
     CDS             complement(25569..26444)
                     /gene="dapA"
                     /locus_tag="PLW08_01680"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPL51344.1"
                     /translation="MLQGTYVALITPFKNGSIDWTALEDLIQFHLDNGTTGILLLGTT
                     AETSALASDEKDALLRFALKKIDGKLPVIIGTGTNNLHQTLASTQKAKELGADYALVI
                     TPYYIKPTQKGMLEYFGIIASKIDIPIVIYNVPGRTGVNITAATTVELAKTYPNIVGI
                     KDASGNLVQVTQIVRDAPENFSVMCGEDALNLPLIAIGAKGTISVTANVAPKLMSEHI
                     ATCLKGDFITAAKQHQYLARLNEMMFIETNPIPAKEALAMMGKITLAFRSPMCPLMEN
                     NRALLKQCLQDYKLI"
     gene            complement(26549..26962)
                     /locus_tag="PLW08_01685"
     CDS             complement(26549..26962)
                     /locus_tag="PLW08_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HPL51345.1"
                     /translation="MKKGFPFVVIVLSAILGIGSRKYGNYLPNFIAEYTGDTMWALAF
                     YALFRMLFSRKRNWEIALLTFDFSCLIELSQLWHPVWLEKIRSTIIGGLLLGFGFRYT
                     DLFCYFAGCLLGFLIFSLGKSRRSNKKLFGEATCL"
     gene            complement(27724..28173)
                     /locus_tag="PLW08_01690"
     CDS             complement(27724..28173)
                     /locus_tag="PLW08_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51346.1"
                     /translation="MKATRANLLGEFTGHLDGLIYYRSKKNGKLYVRRRFKFKNHPAH
                     PVFTKNIQNIYALSPSEGYKQNLKIYIALFNANLPEGKKPLHTWTNAYTTLMFALQKA
                     FPETVNLGTLTRDQIYSQNLPCKSVKQAVEAGLLPLVKSYELLNAEF"
     gene            complement(28624..31413)
                     /locus_tag="PLW08_01695"
     CDS             complement(28624..31413)
                     /locus_tag="PLW08_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51347.1"
                     /translation="MKKYLIVIGIILLIGISLLSTDDAKPASLKGKITEFSASDMPFD
                     DGSGIVLKWKPLDKSYRIINYNIYRGVSPDSLFYIGSIEVDPKLGVLSPELYFYDKGE
                     EPFIEFETAPQKLKKEKQQSHNSPLFKKFPQQPELLATMLTRYNIIGAINNSKLYHSS
                     KPVTKDEDIMAGLKLNRFETILGFPKAGQEYYYTVLAVNERGKYLPYADIQKVIPEDN
                     PPEPSANLSSTYIQDTGVFNFEWLPPSSSEDIAMWEGWLIPLAQKPNNGELLSENWQN
                     SAIPLFQIPNYSGAVNTCYSVDTKEEGINLPANLADYYPVLSYSDYMGQSAAVSAKNF
                     RILNSSQLLKIPQIEVKDKPNDKGDYLSVSMGKPLVFVSQVLYLNKAKTTLRINYEIS
                     NNEHYKIKELRFTVLDSLNKEIGKVSEYYLDKTIHFKLPQQYIAIKNMKIRIAVQTNG
                     NGSFEDYTEQHIVYDEGNKIFRGENIYYQNEPVNKLYYEILTRNGFDPDFLFGERTNG
                     LTRAYDHSIPYESIVYKSILDYDNKGKRLILDPQITIAVDQVNGYTFAVPLFRNKFEQ
                     DLKKQKEELDNLLAEKNKYPANAIPDSLLAAIQEAEGNYNFITKHPAYLQAQKAKTNK
                     EWLKTLLATHNKNMRSYSYKVIKTDGKANFVTTAIYADAKGNTIFYPRSDWFDTTKYI
                     TLAASILLCLFLIYAIIQSRKGNAYIRPIAGLESIEEAVGRATEMGRPIMFVPGWGTL
                     GDPDTVASMLILGQVAKKAAEFDVRLISPHCDYMVLPLAQEIIQNAYNEVGRPDAFNS
                     NDIFFISYDQFPYCAGINGITVRERVATIFYMGYFNAEALLLTETGNQTGAFQIAGTD
                     AITQVPFFITTCSYTLIGEEFYAASAYISRNPDLVSMLKASDYFKATIIIVLLIGTLL
                     ATLNFTGFINAFPIE"
     gene            complement(31522..32124)
                     /locus_tag="PLW08_01700"
     CDS             complement(31522..32124)
                     /locus_tag="PLW08_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51348.1"
                     /translation="MSFELFSNLVAAFLTLCIFSFLYKENPFYRMAEQLLVGVSLGYT
                     LVFTYERIFIPYVWQPIILKHNFVLILPCLMGILYLFRFSRKLGWLSRYPIAFSMIGI
                     GFGVPMGIHAGILVQMRQAMVPLENINLILILLGTIAVLLYFFFSKAHTGAYGKFVGL
                     GKWYMMIGFGASFGLTVMARISLLIGRIQFLVIDVFGLMK"
     gene            complement(32141..33322)
                     /locus_tag="PLW08_01705"
     CDS             complement(32141..33322)
                     /locus_tag="PLW08_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51349.1"
                     /translation="MNFFERMQSLDRRWIYIIVALAIIIPLIIPYNSDNVTSPPTENL
                     YQMIDSFAGREDRAVLLSFLHDAATMPELYPMEIAIIRHCFERNIKVFALTFLTSGAP
                     IIDYAFNSVKEEYPDIKSGVDYCNFGYKPQPMAVVLGMGDNIANAVNTDAEGRKLESL
                     PIMKGITNYNEMNLVVEFSGSSPGVYWIYYARPKFGVNVALGVTAVMAADEYPYLQSG
                     QLIGMLTGLKGAAEYEKLVDVFAAYRDPAIDYSVKTDAEGNKILPGRPFGKEVLNDES
                     TKKLINITTQTKAEFTPEEYTAFVAKYPEQKAIFDQLKEEQNGTIIIDVTKITPELRN
                     QMGENAYREINRLTHNISYKFKVARIGMNAQSVAHIMIIIFILLGNIGYFIQKAKTAE
                     K"
     gene            complement(33341..33970)
                     /locus_tag="PLW08_01710"
     CDS             complement(33341..33970)
                     /locus_tag="PLW08_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51350.1"
                     /translation="MKKTIPLIIVIVGGIIFVLYALSPHKLLQDDFYNKFYLPWVYAS
                     SPWAALLGVISLCMMHSSKIKRKAPKWQYSYFFFAGFIITSLAGFIGGTQKGSVLMWI
                     FENMQMPMSSTMFSLLAFYMASAAYKAFRARSVEATVLLLAAIVVMLAQVPLGVKIYK
                     NLPIISQWILDVPNLASKRGIAIGVGLGGVATSLKILLGIERSYLGGTD"
     gene            complement(33983..36910)
                     /locus_tag="PLW08_01715"
     CDS             complement(33983..36910)
                     /locus_tag="PLW08_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51351.1"
                     /translation="MRLFRSRLIFLLLFLLLLLSTVIIAQKQSDAERYVVEDFKVEDI
                     PADDGTGLMLSWKPLERDKRIIEYRIYRGVSPDTLFFLDAIPVNVKTGVASERMFYYD
                     NSGSEFFDISSPRKLKKEKQQGKNSPLYQKMPRNIPFLAEISGKFSLLSVSEKNKYYY
                     SSQKVTLDKPQEQGSENEKQEEGETYAGLKDNQQTVYCFLNPGEKYYYTIVAVNERRQ
                     FQKHAKITAGSPEPNAPEPATALHSVVLEDSRELRFEWELPLYNSDIAQYRIHQTPLF
                     TPTEWDSLRLHPELFQTKTVVLQSGLVGYGSGKNYSQVSIPEEASLEAYINSAYTLEL
                     IDADGFSSFSALSKPRITHSKDLPAKPIIKMEDKPNDKGDRLTVVWDHPICFVVKTTA
                     LNNKFNKFRVNYQLNKTETQQVDNIYFEFYKKGEEKPFAKINEFYQDNSVILKVPNNY
                     DYKKGFRVKITLAGKPEIPKDYSIEQDLEYDPNMLAILPTKALYRNGKDVSKISNVVY
                     RKNMASKQLTLVKRNTSYDNSLDVTIPYATMIQKPVNGFTFAQGDTLITYINGERQAR
                     KLKPGDIKTPLALISPNIDLVYDKENETRIETNIFPDLGRKEAQEKVEELTKKIAELK
                     DKITGETDPAVVEQLNQNIEQLQKQLNAYSKNEHLVNANKITNRKSRMRYIAKVREDY
                     SRYSTYQVVRTNSRGLFTESDLLKDGEDIFYMKPVSNVFDTNKYTTLVAMIIFGLMVV
                     IFINLAKRGKDLYIRPIAGLEEIDTAVGRATEMGRPIMYMMGYGSLGDVATIASMGIL
                     SLVAKKAAEYDIKLIVPVYNYIVMPVVQEIVRDAHYSVGRPDSYDKNSVFFLTDVQFA
                     YVAGVNGIMIRERAATNFYMGYFAAEALLMTETGNGIGAYQIAGTDAITQIPFFITTC
                     DYTLIGEELYAASSYLNREPMQLGTLKAQDYYKFLIFAFVIAGAVLSTFQLTGLSELF
                     PLK"
     gene            complement(37180..37683)
                     /locus_tag="PLW08_01720"
     CDS             complement(37180..37683)
                     /locus_tag="PLW08_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPL51352.1"
                     /translation="MKTYRFTLPLCPADIAQLHISDKVLLTGCLFTARDVAHKRMVDI
                     LKRGEKLPLNLAETTLFYCGPSPARPGNICGAIGPTTSSRMDIYTPLLIQNGLRVMIG
                     KGERSIEVQKAIMDFGAIYFVMPGGISAYLSQHIVSCETFLWQELGPEAIHKLWVQDI
                     PVYVAIK"
     gene            complement(37687..38538)
                     /locus_tag="PLW08_01725"
     CDS             complement(37687..38538)
                     /locus_tag="PLW08_01725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPL51353.1"
                     /translation="MAYRYVSHETIRSELANALKYITFNPDPELPALLKNALANETSE
                     ISKDILQCMLKNIELAPKIEIPLCQDTGSLVVFAEIGNHCIINGPPLPEIINETLIKS
                     SAELYLRPSILKDPLLQRENTGNNAPAVIHISIVEGDKLKLQIAQKGGGAENMSQLKM
                     LTPSAKADEIKNFVVETVTLAKGNACPPLIIGIGIGGNFETCALLAKKALFQPLTAKN
                     PIERYALLEQEILKGVNETGIGVQGLGGKTTALAVHILNAPCHIASLPVAVNLQCHSH
                     RHIEIVI"
     gene            complement(38542..39420)
                     /locus_tag="PLW08_01730"
     CDS             complement(38542..39420)
                     /locus_tag="PLW08_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPL51354.1"
                     /translation="MPLLSEEALVRLNKFQLTAKSIVEGFLVGLHKSPYHGFSAEFSD
                     HRQYNPGEPLKDLDWKILAKTERYYVKRYEEETNLRSYILLDHSKSMFFSSGKSTKIE
                     YATQLAGALAWLMISQKDAAGLYTFNNKITSAYPPKAIRSYTSQLFAALLDLKAEDQT
                     DLLTPLHQIAELVKKRSLIIIISDLLDEPERIMEALKHFRARKHEVLVFHIIDPKEEK
                     FDYKREAQFIDSETGEKITVSPWQIRKEYVENFHQFAEYLKRECYKNQIEYNPVDTYT
                     PIEDLLLKYLIKRQKG"
     gene            complement(39422..39622)
                     /locus_tag="PLW08_01735"
     CDS             complement(39422..39622)
                     /locus_tag="PLW08_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51355.1"
                     /translation="MNNKDDEKNKLQLNRTNFILLLVAAIVLVVGYIIMSFNEITISP
                     ILLVIAYAVIVPFALLYHPKKK"
     gene            complement(39656..40480)
                     /locus_tag="PLW08_01740"
     CDS             complement(39656..40480)
                     /locus_tag="PLW08_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL51356.1"
                     /translation="MILRKIKCILWHIYLISEFFLVSAAIRIFSADPVLRRKRLLKNN
                     TRISKRFIHAFNIKLTINHSENLQKLKDIPYLAVSNHTTYLDIILLSAVENFVFITSV
                     EMRKNPFLGRITKSGGCLYTNRKRYISLPAEIEKFASAIHQGFKVVLFPEGTSTNGIT
                     VQPFRRSLFQVAIEAKCPILPVCIKYKAIDGKKIDKKNRDLIYWYGDMPFLTHYLKII
                     GHSLEAQIDILTIIPYQEGKTRQELANQVYGQILSSYLEQESSVDKQSNSLSMVLK"
     gene            complement(40893..43622)
                     /locus_tag="PLW08_01745"
     CDS             complement(40893..43622)
                     /locus_tag="PLW08_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51357.1"
                     /translation="MKRTLCLVLLCLPLLILATTLKTTIQFPVPQEGLSQSALFKQGY
                     GFSIAPGTLQLPVKQINILLPKDAVVDNWQVILEAEATFTEEPPAINSAFSNGEEILT
                     SPVNRNIYSRYRYMGLKKWGELNYACFNVLPAIYNGSHWLWSPSCQITINYTSSAKAK
                     GIIPPTYTDAGFFANPQNLSQWYTTSKEGNNRLLVITTPELYTALDWLVTFRQSQGVD
                     VSFCDIALALASGTGTDNAEKLRNYLQNTYASTPFSYLLLVGDYDTVPVAYVTPEPNG
                     QETVPTDFFYADLSSNWDTDNDGRRGEYSTGYMNEDYGIDFTPEVFVGRISTNNPTNV
                     SAIASRIVAYEQSTEPWKEKNLLPAAFLNYQGEPEPVYLQTDGALFMEFMRNTCLAEQ
                     ENFTMYEQEGVVLSFPCDLPVNYDNLINKLNSESWGFINWDAHGSSTNSSRKVWINDY
                     NQNNVPDENEMQWMNLVNRQSFDNLENTSGTIIYAASCYNGALDSNNYCLAEYALIKK
                     AVGVIAATRTGWYKVGWQNPGWGGLASYDYHFVENFRQAKLDLGSAHSWANLLHTQYY
                     LFGDPVDNGGIIYPELQNVYTYMLFGDPMVGWTPNQINPAGEILVWEPEGNEGLAVVN
                     ALRKISNFNVIYSDKLIPDYDYLNNFEAVFYLAGNSLPETELEPTSYEYSYLNNYLEA
                     GGKLYCENYIDDSYGELFTKMGVQMADNNPLNITSIFYPSSNLAWNYNGADSSVYPII
                     PAQTTAQGIFFASSGNTNNPVIGILNATDNYSVITSSFRTTQIESGEYTLNNMIGIIL
                     SELNVMDYNPVGNNDPIMVPVIQSVSVYPNPASQSSSISFNLTKSAPVSIAIYNIKGQ
                     KVKDLIGSELKSGNYQFTWNGKDDNGRYCSSGVYYYRIVTPEKNITKKMLLFK"
     gene            complement(43644..44480)
                     /locus_tag="PLW08_01750"
     CDS             complement(43644..44480)
                     /locus_tag="PLW08_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPL51358.1"
                     /translation="MNIRQDFARIAVILVEPIYGGNVGAIARIMHNFCFSDLRIVGKV
                     PEKNDFYLAMHSEEILENVNTYSTLEEAINGLDRVIAFSRRLGKNKPVDFTPGQMAKY
                     VHSLPDSKIGLVFGRETYGLTDSEAELCPLRCHFMANPSFPSLNLAQAVALALWEIYS
                     LPWEEKQKGEKYYNSASGKELDEIRNYILEVMRQCGFFQRRETINWDAFLAKMLAQLN
                     PDKNMLYRLRQMFNRFNVLVTGKGLGYELPNSSSEKKENKKNSELNNSCSNASRPCNP
                     DT"
     gene            complement(44477..45367)
                     /locus_tag="PLW08_01755"
     CDS             complement(44477..45367)
                     /locus_tag="PLW08_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPL51359.1"
                     /translation="MIKEVGRLNLDDYKILDTEVEKIFRNIEKGKDALAEKQITDLGN
                     TANYFVREELGKRLAQYSGNGEMDAICGRLLDHFIYGVRATALFYFYYKHQDHPEIII
                     KTVEKTFESVPWESETICFELWKKAPNVMQEYMPAWAESDNEKKRAISMHGMENIASR
                     HPQYVLSFVSNLLDDESEEVQKKVCHILTQVGRQKPLQCYNSIRRWLIEGDDTRIRIL
                     WQTMKKLANIYAQRSRRDKTNEFMNVTQKTIAEWRNDPDPKVATMGAKLSGIIAKKKK
                     RENQNQKAANSENNYYSRQI"
     gene            complement(45690..47033)
                     /locus_tag="PLW08_01760"
     CDS             complement(45690..47033)
                     /locus_tag="PLW08_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL51360.1"
                     /translation="MKNVKTIALNQLINYIANDPLNKLPKIMELAKKLDRKGTYLKLF
                     TVITDALSDETSHWHKFVENLFNNVDSKVIHKLAECFAINTTLVGRPIREKYKAKYSI
                     QVPWAILMDPTSACNLSCTGCWAALYGKQHNLPFEVMDSICKQGKKMGIYWYLFSGGE
                     PLIRKNDIIKLCKKHQDCFFLSFTNGTLVDEKFCEEVLNAGNLTLCFSIEGNEPATDM
                     RRGKGSYKRVIEAMELMKKYRLFFGYSTCYHRYNTESVASDEFVDDMIARGCFYAWNF
                     TYIPIGKNAQPDLIATPEQRAYMYKRVNEIRTHKPILALDFWNDGELANGCIAGGRSY
                     FHINAAGEVEPCAFVHYSNVNIKEVSLLEALQSPIFKAYQKRHPFNQNLLQPCPMLDN
                     PEKIMEIVNEANAKSTDIEAPESIEELCAKTKPAAEKWAPVAKELWENREKNSST"
BASE COUNT        14469 a         9477 c         8870 g        15603 t
ORIGIN      
        1 gtctggagga aagtgaaaag gggaaaaggg gaaacccaag gcaagcatcg taaaatattt
       61 atgtcatttc ttgaataaaa agtcatttga cattcctgca aaagcaggaa tacacataat
      121 ataagaaaat attatccctt ttcaatatga aaattatatt agatagaaag atagataaca
      181 aagaaagtct gtagccattg tgtagttgga acttgttgaa gttctgcatt ttgtctgtta
      241 gaacttttta gtgtaggttt tttgaggagg aacagcgtcc tccgtctaca catattatcg
      301 tcacgattcc gcactgacaa acaaatatgt aaaaatatta aaagttattg agacacagac
      361 gactcctgac ttacattgaa attaaattat tatcaatcac tttacttctt attattacta
      421 ctttcgtatt tatcaaccgt atcaagtagc cgtttagaat aatcatatat ttgttctaat
      481 gaattaagtt tatgcctttt cctatacttg tcttcatcaa aagttgttaa tattattgta
      541 tttttaccta tctctaatct acaaataggt ttttggattt tgtcatctag cataattaca
      601 aaatatgact tagtatcttt ataggtaatt ctacttagag caactcgctt agaaacaatg
      661 gctttaacta tatagaatgc ttctatctct tccattgttg tatttatccc atcatcttta
      721 attatattgt ctttttcttc ctttttctta tcagtttctg cttccttttc tttaattagg
      781 acagttttta atctttctgt aatgatatca cttataactg aattcactga ttttttaaca
      841 agctcagtga attgttcaat gatcttgctt gttagaggtt tagaataaat attttttgct
      901 aacaaacgaa ccattggttc acttggatta attatttcat tatgaatcat attcttgagt
      961 tcatttgtat attgcaattc actagcagtg ctaagaatat tatcaatgtc aaaataggat
     1021 ttatggaatt tttttaattc ttcaatttga ttttctctaa tatctgtaag gtttatctca
     1081 aaaaatggct tttcgtccat tttatttggt tctttcaaat cagtataaaa gcgataaata
     1141 attccacttg taagaatgcc aaactttgct tttgacacat gaaaatatct aagtagttgg
     1201 ctttcgtaaa ggtttaactc acttttccaa tgtttacatt caataaggat aataggttta
     1261 ttctctttta agatggcata gtcaattttt tctccttttt ttgtccctat atcacataca
     1321 tattccggaa taacttcaaa agggttaaat atatcatatc ctaatgcctt aataaatgga
     1381 agtataaagg cattttttgt agcttcttcc gtttgaattt gatctttctg catttcaatt
     1441 ttgtcagcta tttgcttaat ctcatccttg aaatccattg gaacctccaa taactttttt
     1501 tcatcctttt tatcttgaaa gattgtgtca actaaaatat tatttctatt ggcttttctt
     1561 tctctttagc ttaaatatta ccgtgaatat aacacataaa attcaaattt aattgtctta
     1621 atgatgtgta gtcggaattt gctgaatctc tgcattttgt ctgacagaat ttttttattg
     1681 tagttttttt tgctgaggaa cagcttcctt ggtctacaca ttttatcgta aaaaaggtga
     1741 aacacgaaaa ataaatagag agtgaatagt agtattcttc ttttcccgag gggcttacgc
     1801 caccctctac ataaatgtgt ccccctttct ggctttccta ccctctcgtc atttctcttt
     1861 ttctcttttc ctctttgtaa aaatcaaaac attgccttca cgatttcttc cagtttcact
     1921 tctccgaaaa agcttttata ttccatatct tttagcactt ttgctacttt ctcaggttta
     1981 tgttcaattc cgcagagggc attttccagt tcgctaattt cgcggttagt aaaaaaatcg
     2041 ccgtagatat tcacttcttc aatgatacct ttatttacca ggaggtagaa ttcaattatt
     2101 cctacttttg ttttaaggga atggcacaaa ttatagcggg gtgatttgcc aaagttccat
     2161 tcccaagtat catatttact gttcattaga gcttggattt cctctttatc tctgagggaa
     2221 aaagtataat cctgaatatc gggatacata gttctcactt ttgccctaac taaagtgaca
     2281 aaatcctgca aagaaagagg ataaggaagg tggtcggaaa catttgtaac tcttgcctgc
     2341 acggatttta ccgctttatc gttaaacttt tcttctctcg gttttagagc cgcggtgagg
     2401 tctgaaatat tggagctgaa aaggatagtt ccatgctgga gagtttttcc gaaagcattg
     2461 gttttagcgt taccggaaaa cttgcagcca ttgatagtaa ggtcgtttct tccttctaaa
     2521 acggcaggaa ttcccaactc ctgaaggaca gctaaaatgg gtttggtata gcgttcaaaa
     2581 ttacggttaa aatcctcact ttcattcatc aaaaaggaat aattcaagtt tcctggatcg
     2641 tgaaatacag aacccccacc tgttaaacga cgcacaaccg gaatattatg ttcccttacc
     2701 cattcgtaat ttatttctgc caaagtgttt tgatgtttac ctacaataat actggggttg
     2761 tttatataaa gcaaaaatac atcctcagga aagttcttca ggatatattc ctcgcaggca
     2821 atattaaaag gggcatagtt actggggcta aaaattgaaa gcatattcat cacctaaagc
     2881 agttttgcag gtattcaaat tccctctggg caaagaagaa actgccaatc cgtttttacc
     2941 taaacagtat atttcgggtt tactttttat acctcaactt tttcattttc aggacaaaca
     3001 gagctgataa actcatcatc gcaataattt cccaaaagct aattactgga tgaaacttaa
     3061 tgcgctcttc cttaagggaa aaaagcccta ccggaacgct atgaatgcca atacctccgc
     3121 ctccgctgaa accaacttta atttttacct ttttgccttt ttcttctgct ggggatgagc
     3181 tgtcttcctc ttcttttttg gcagtatcgg gagaggattc atcagctccg ccacccattc
     3241 cataagtaac tctggctact ggaattacat aaagatcacc caattttgag ggctgtccga
     3301 aagaataacc accacccaaa gcattatttg ctatggagcg gatttgtgag agtatttctt
     3361 taatatccat agtcaactcc ttttcttata tttctaattt ataagctttg atttatttgt
     3421 caagtaccaa ttactctatt tgcctatagt aaggtgctaa agcaactatt gaatcaaaga
     3481 tgcctggaac tcgcggttaa ttatatttag ttttatttca atacaatcag tttagctgtt
     3541 tcacacctgt cctttgtttt cagcttacaa aaatataagc cgttggcaac tgttctgttg
     3601 ttgttatcta aaccattcca agaaatttcc gtttcacctt tttctgctaa accattaaac
     3661 agagtttgca ctttctgtcc tttgatattg tatatttcca ggcagacctc ctgtttttta
     3721 tccagattca gctgcagggt agtagtttct ttaaagggat tgggatagca acctattatc
     3781 tttgcttggg gaagatattc atcctgcaca ggaatatcag gaatcaaata aaatgcggtt
     3841 ttcggcattt taccatttaa ttcattataa acaggaggat tataaggatc gggatcataa
     3901 gcatcggtcc agcgttttaa agctctcatt tccggaaccg tttgcgctaa aaaaggatca
     3961 gtgcaggcaa gagcataagt atgtgaatga tagaccatta cacacaaatt gccctgctga
     4021 tattgataag gagtagaaaa aggaaaatac acttcattac tttcgggtgg cataataata
     4081 taggaattga agacaggtgt caattcgcct gcagcaatcc agccctcatt taagttagtt
     4141 aaattggttg cccctaccca gatttttacc aatagattgt gagaaaaagt ggaattcata
     4201 taataactaa ttccataaat agttccgcta ttaccaattt cctcagcata gtaaattgtc
     4261 tcgtaaaggg aatatttcca gtaaacatct ataggaattc tttgcagttc atctcctgaa
     4321 ccaacaatga taccggtatt gggattggga cgtataatgc aggtataatc gccaatatta
     4381 tcctccggat attcatcttc gggaagttct gctattgcag ttaaatgttt ccagccgctg
     4441 gaatctatag ttgccactaa atcatataaa tggctgtctg ccggtgccag ggaaatgcca
     4501 tttaaacggc aaagttccgt taacccacaa taaagaacca catcatagct atcaactgtc
     4561 tcggttccat tgttttttac cgtcactgga agaggaaaac tttcatgcag attatgataa
     4621 attccctccg tataaaacat actatattct atcttcagag aaagatcata aagcacaggc
     4681 ggcaaaggat taaaaatgta aaccgttcca gcgggaataa tttcgtttat agtatagtta
     4741 tcctgcgtag tagataccgt aggagggttt ccgggagtga tactgaaaaa attgcctgct
     4801 cctgcattgg gcatattaat tccgatggaa gcagaacaat ttagcggaga gccatttgtg
     4861 tttccatagc ggaattcaat tttgccgttt tcggaaagca ccacttgaaa gtttacataa
     4921 caattaaaat tattatagag ccagcgagca ttggagtatt gaacccaaaa ttcccggttg
     4981 ggagctgtgc caatggttgc gtattgcaaa cttccttccg ccaaactaag atcatcccat
     5041 aaaggagcaa ttatcggata tatgccataa tgtaactgat tattggggct gtcatcattc
     5101 cagttggcac caggattgat aaaaccatta gtggatattt taacatcttc atatatctgc
     5161 ccgcaataag gaaaggaaaa accaatatct atacctgaac taatgtaatt attcatatct
     5221 ggaaaagtaa gttgaatccc atttatagga ttgtattcag cagttgtgta ggcaaaagtg
     5281 taatagcgag aaatgccgct gtttaaaaat gcactgctta aaagtagaac ggcaagaatc
     5341 aaaaccttat agcgcatcat agctaacctc cttttttaga agttaatgca ctatacatta
     5401 cccattttta tctatatttt ctttactaat ttccgtcaag ctttttattt tataattcct
     5461 atccgtttat tacccatcct cctctcaacc tccactcatc ctaaagttgg gttgatgatg
     5521 gaaaacaaga gaggaagacc aaagcaaaaa atagaaaaaa aacaatatgg caagatggca
     5581 agagaaaaac aggtccataa tgggaaacca gatacaaaaa tgcagaaatg acaattgcaa
     5641 attgagaatt aaaaattacc ctccgggtta atccttttaa tccgtaaaat ccgtgttgtt
     5701 aataaatatg cgagcttaaa ttcccgtttc tctgtaaagc tgaattattt cctgttccag
     5761 ttcttccggc accatatctt taatgctttc cccgtttttt atgcgctgtc tgatttcagt
     5821 ggcggaaata gggcaaagtt caatatttaa cactgttgct ttcagctgac taataacttc
     5881 cggcagaagg gcatagccag ggcgtggtaa aattaaaaaa tgcaattcct ttgccaaata
     5941 aggataatca taccacaaat gcagctcttt cagattgtcc gaaccgatta caaagctgaa
     6001 atccacatcc ggataccgtc ttttcagttt ttgcattaaa tgagccgtat aaccgctacc
     6061 ttcctgatca gcatcggaaa tagcaaattg aggattgttt ttaattgctt ttttcactaa
     6121 ggcatagcgt ttttcaaagg gaagaataat agagtttttc ttaaaatggt gatgaccgct
     6181 ggggacaaaa agaaccgttt ctgccgcttt ttgttgcagg atttgattgg caatatgcag
     6241 atgacctgaa tgaacaggat caaaactacc ccccaaaact gcaaccttac cctgcacttt
     6301 acttttccag cttggcgaat tttttggcta aactttttgc ctctttggaa tcgggatagc
     6361 gatttttcag gcgttcatag ctcgctttag ctttgtcgta attcttttga tgaagatgca
     6421 gcttggcgga atagtagagt gattgacggt ctaattcatc attattgccc aaggatacaa
     6481 tttcgtcaaa atacatcaaa gcgctgctat agtccttcat tttatagtag atataaccgg
     6541 ttaagtattg cttttccaaa agcttatatt ggcatttatg aatatagtcc actgcctgaa
     6601 catagcgagg atcattagga tagcggtcta tgaaggtctg aaatgcttct atacatttta
     6661 tggtctcggt ttgatcatac tgcggaggca gtgattcttc aaacagacat actcctatcc
     6721 gaaaataggc atcggcaaca ttttcgtgat caggaaacga atttataaac tgctgatatt
     6781 tggctcgcgc atcactaaat ttattcatat tgaaataaca atcggcaact ttcattgtag
     6841 cataggcagt tgcagctgat ttgcgttcaa aagatatctc ttcatagata actgcagcgc
     6901 gggaatattt ctttttggca aaaagctcat cggcattttt cagcttggct tcggtagaaa
     6961 gctgtgtttt attactgctg caggaaacaa ggcaaaaaac ggtaatcagc aaaagaaaga
     7021 tgtattttcg cacaatatat tcctttttta atcaaaattc cagaggagaa atatgaggga
     7081 accaatagct gcacctgcaa ctaagggctc aaaccaacga aaatgaaaag aggaagttcc
     7141 tgaaacatcc ggattgggac gcggtaaatt atgggtggaa atttttagaa tctgctgagt
     7201 aggaagttga atctgcctaa tttcaaaaat gtggacagga taccgttcag tgcggtagga
     7261 gaagaaactg ctctttacgg tttcctgcca cgcaatatta agatttatct gaaccagcaa
     7321 tgctttttcc aatccaagtg ctgaaagatc ggggactttt ttttcttccg gggaggaaat
     7381 acccgcattg gtttcagata agttataacg caagtccgca cctttttcta aaagattggt
     7441 actcaatttc tgggttaaaa caggattcca ctctccgcaa ttgatgtcta aaaaaatggg
     7501 ctgcccggtc tgaatttcag ttgctatttc tgttattatt tgatcaaggg ttagggtttc
     7561 ttggaatgaa aggagttttg aaatgagtaa aagaaaacag acgggcagca aatacttttt
     7621 cattctttaa tcggcttgta aaaatgcctt atagccaaga taagtagaat ataaatccgc
     7681 tttggaacac aattcataca gcgaatgcat tcccattaaa cccactccgc aatctaaaac
     7741 ttccgctccc aaattggaaa gaaaataggc aatagttccg ccgccacctt catccacttt
     7801 tcccagttcg cctgattgcc aaaaaactcc tgcctgattg aaaatgcgga taactttggc
     7861 aataaattcg gcattggcat cgttgcaact atattttcct cctcttcccg taaatttgga
     7921 aatgccgatg ccataattga aaataacggc attttgcttt tcgtgcacat taggataatt
     7981 gggatcaatg gcagcagtta catcagcgct taatatctgg ctgtttatga aggttttacg
     8041 caagttggca ctgctgttgt cttcattatt atgagccagt aaatcggaaa taaagtcctg
     8101 aataaaaata ctttgagcac ctgtatttcc ctgactacca acttcttctt tatcggaaaa
     8161 atagaccacc attgtgcgtt tgggcttatc aggaaggtta tttattaaag ccatcaaggc
     8221 attataggca caaatacgat catcctgtcc atatccaatt acaaaagaat tatcaaaacc
     8281 tgaatgacgc gctttaaaag ctggaactaa ttgcagttcc gcactttgaa aatcagcttc
     8341 ggtgatgccg tatttttcgt gcagctgtct taaggcataa ttcttgattg cttctttttc
     8401 ctcattgccc ggttcctgca ttcccgaaaa aacaagattc attttaccgg cttctattgc
     8461 ttcgtctaac ttcttggtat attgttcctt gcctgccaaa tgaggcaaaa gatcgggaat
     8521 cacaaaaacc ggctcatctt ctttttcccc aaggcaaata ttcaaaacgg agccgtcttt
     8581 tttaataata actccgtgca aagccagagg agtagaaacc cactgatatt ttttaattcc
     8641 gccataatag tgtgtccgca tacagcttaa agcgctatta ttatcttcat aaaggggatt
     8701 ttgtttcagg tctacccgag gagaatcaat atgggcagca atcatattaa atcccttgga
     8761 aattggttca ctgccaataa cagccattgc cattgtctta ttgcgaaaaa cgctatagac
     8821 cttgcggctg ccttttttac caataggtga aaacttattg ttcttcaata tttctaaggt
     8881 ataggcaatt gtttcgcgtt cggttttggc tttattcaga aattcaatat agggaatggc
     8941 ataagccatt gccttttttt gttcctcagg agtagcttct ttccagaaat ttcggggttc
     9001 gtaggtaagt ttttcttgtt ttgtcttttt cattatatct ccagttatct atttaatttc
     9061 tattatcttc agttttcttt ctccgattgg agctttaaca atggctattt cacctatttt
     9121 tttgcttaat aaagctttgc cgatgggaga aacaatagag acctcctgaa tgccttcttc
     9181 attgtaataa ttcagttctt cgggacctac cagatggtag cagatacatt ctttggtgtt
     9241 aatatcttct gctacacaat aagttccgaa acgcaccata tctttgggaa attcactggt
     9301 atctatcact ttcaaattgg cactgcgtct tctaagataa tcaatttcat tatcaatagc
     9361 tcgttgtctt tctctggctg ctttgtattc tgcattttca cttaaatccc caaattcgcg
     9421 cgcaatagct acagctttaa taacttccgg gcgttcggca agcagttcat ttattctttt
     9481 ttgcaaccgt tgcattccgg ttttggtaat aaattggctt aaatccaact tttattgagc
     9541 tccttttttc tttttcaaag tgcgttgagc agtagtagcg gcttttttca aacgggcatt
     9601 tccgcttttt gtccaaatct ctacagggct ctccagttcc ggataataat ccgttattga
     9661 agtgcaaagc aattctacga tttgtggatc atgactgaaa ctgaattcct cccaaatttt
     9721 taagtaatat tcaggattta gcttggcaac cgtttttagc attgtaatat gatagggaac
     9781 cgcttctccc aaaggggata accactgcct ggaaaaaaag gtcataattt gaggaatgag
     9841 gtcttcgcgt cttttcacca gttttatcaa tagattcaag gcggattttt gaagagcagg
     9901 atttgaacta ttgctgtatt gaaaaacctt attcagatat ttttcaggat acttgcgtaa
     9961 aagcggcaac ataactttat ccaataaagc attgatttct gccatcgtag cattagaaag
    10021 agcttcttct aaaagaggca aataaacaga gggcttacga ttaagcagtt tgcttaatat
    10081 ttgaccaaag gcaagcttac cgtaatctcc ctttttattc cataacgctc tgtaaaatgg
    10141 aagataagca tcatgctttt tccccagttg ctgaacaata ttgcttgcta catgagtgat
    10201 aacttcagcg ggaatctttc catttaccct ttccggatat gcaataaaga tgatttcaaa
    10261 atcataatca cctttcggca gtttattttt caggaaatca gctgtatcca tattcagccg
    10321 ttccttccag tcaatggtaa gcatttatct ctcccgcttt tactttttct ctttcttttt
    10381 tccttatgcc tgaaaatcct tttacctgtc aatagaaaaa tataactggc ataatattaa
    10441 agaagttaac tcgttcctat cattaaaagg taaaccctcc aaaacaaatc ttcccttttt
    10501 taaaaactgg ctgggatgga aggagttgaa ccctcacata cggatccaga gtccgctgtc
    10561 ctaccattag acgacatccc attatttttc tggctgggca ggaaggattc gaaccctcgt
    10621 atggcaggac caaaacctgc tgccttacca cttggcgact gcccagcaat tttttttata
    10681 aatggcggag aggcagggat ttgaaccctg ggtggacttt taatccacac acgcttagca
    10741 ggcgtgcacc ttaagccaac tcggtcacct ctccccttat tttttctgcc ttatttttct
    10801 ggcggaggat gagggattcg aacccccaag ggcataaccc ggcggttttc aagaccgctg
    10861 ccttaccaat taggactaat cctccctcta tattttatcc ttgaaatttt tataaggggt
    10921 ttctgtcaag ccctttcttg ccagttcaag gaagttagaa aattgtatct ttacggaaag
    10981 ggagaagagt gtctattatt ttttatcacc tttctgccaa agaggaattt gacagaccac
    11041 tgctttttcc ttgacagcaa aaataagcac aggataaaag gaaaatatga ataatgaaga
    11101 atataaggca ctggatgaat ttgagctgga gcccgaaaat tgggaacaag ccgaaagaac
    11161 tgaaaaaacg gcttttttag ctgctttggc aaattcaaga gaaacagaca aagacatcaa
    11221 ggaatcgtta aacgaactgg aacgcctggc tgaaacggct ggaattcagg ttttgggaac
    11281 ttatcatcaa agaagaaatg ctcccgaaag aggaacctat tttggcaaag ggtttttgac
    11341 tgatttaagc cgaaaaatga tgcaggcaca ggcagacatc ctaatcgtta atgaggaact
    11401 gggtccttct caggcatata atattgaaag ggactatcaa ataaaggtta tggaccgcac
    11461 cgaagtcatt ttagatatct ttcataaaca tgccaaaaca cgggaagcca aactacaagt
    11521 tcatcttgct gaactggaat atcaattgcc acgcttaaaa agaatgtggg aacattttga
    11581 taaagaacgc ggtggagtga gaaatactgg cggcactgct acaagaggaa tgggtgaaaa
    11641 acaaattgaa atagacagac gcctgattaa agataaaatc cgaaaaatca acaaagcaat
    11701 agaaaccatc cagcaccaaa aagagacaca acgcaagcaa cgcgaaaaag caaaaaagat
    11761 ttgtttagtg ggctatacaa atgccggtaa atcaacactt tttaaccagt taactaatgc
    11821 cggtgtgctg gttgaggata aactttttgc caccctggat tccacttcca gacagctgaa
    11881 actttccacg ggaaatccag ttgtgctatc ggatacggta ggttttattt ccaaactgcc
    11941 tcatcatttg attgcttctt tcaaggcaac tttgatggaa gtgcaggatg ccaatttgct
    12001 attgcatatt gttgatgttt ccgatgagcg ctttgaatat tacattcaac aggtgaattc
    12061 ggttctgcaa caaattggag ctgaaacaat tccgcagatt ttggtcttca ataaaataga
    12121 caatgtggat tcaatattgg tgtctctgtt ggaaagccgt tttccggaag gagtgtttat
    12181 ttccgctatt caaggcataa atatagacaa attgcttgcc aaaatggaag aaattctttt
    12241 gggaaacaga atattacaac taaaaattcc ttacgagaaa acggctctgg tttctaagat
    12301 gcacgatatt gcagaaatta ttagtgagga ctataaagag gatggaatct atctgaaagt
    12361 ggaaatcagc agggatgaca ggtttttggt tgaggattat gtgctggaaa atgaagaagg
    12421 aaaaaattga ggtgttgtta aaatagggga ttataataaa aaaaaccgtg ttaatccgta
    12481 aaatcagtta aatccttgtt gctaataggt cgtaaggatt ttggttaaga gcaaagagct
    12541 gaaagcatct aaaccttctc cacctttttt acccaaattg ggcagtaaaa ataatggtta
    12601 atattttttc cttaatcatt aagcttatat tttagtaaaa atcccgtaga ggtgaaagtt
    12661 agataactac aggttttaac cctgcaaaaa gatatcgctg tgaaagagaa agagttctgt
    12721 aagaaagaca gatattatgg tggaagttta atcctgggca gaaaagttaa attttatatt
    12781 tagattgccg cattctgttt cactataaat agaggacata tatttttcag agtgctacaa
    12841 actctttggc aaagcggaag ttgccaatct atttttcctc aattgcctta ttcgggttta
    12901 actatcaaaa aaatgttctt accaaataac attactgaag cattgcttac ccattgctaa
    12961 tggcattagt aataggtaag caatgggtaa gcaatgctcc taaggaagaa aatataacaa
    13021 attataatat agtaaattag caaaataatt agccgtgtgt tttgatgatg ggcaattcca
    13081 gaaaaataaa gaagaaaaaa attgagttgt tgttaacata ggggattatt agtgttaatt
    13141 cgtaaaatca ggtatattca tattgataat aggtcgtcaa gagtcataag cgccagtaaa
    13201 gttatttctg aagccgaaat tttgaaagca ctaatgactc ctgacttaaa tttttacggg
    13261 gggtttacaa ggatatcaac cttccggaaa tctaaacctt ctacatcatt gtgtcgccct
    13321 gacgggcttt taaaaaaagg aaaggaaaaa agaaaaaact cggggcttct ttcttccgag
    13381 gggcttacgc caccatcgct attattgttt cgcccttcgg gctttttggc aaagagcgaa
    13441 gagctaagag cagagagtgt tttcccattt tttaacgagg ggcttacgcc accatcgcta
    13501 tcattgtttc gcccttcggg cttttaatgt atctaaacct tctaaaccct ctcaaccctc
    13561 tcaaccctct caactttttt tgttatcaga aggtattaac tctttcaggg ataatcagtt
    13621 ctgcttcggt agcttgcacc acatcatcaa tggtaagtcc ttcggcaatt tcgcgtaaat
    13681 aaagtccctc agggcaaact tctattacag ccatatcggt aataatgagg tttaccttcc
    13741 ctttagcggt aagcggtaaa gtgcattttt tcaggatttt ggagttgcca tatttatcac
    13801 agtgttccat tgccacgata actttttttg ctccagtcac caaatccata gctcctccca
    13861 ttccgggaac catttttcca ggaattaacc aattagccaa atttccttcc tggtcaactt
    13921 gcagagctcc taaaacagta gcatctatat gtccaccccg aattatagca aaagaggtgg
    13981 cagaatcaaa aaaacttgct cctggaatac aggttatgta acctcctccg gcattgatca
    14041 tatctttatc ttcctgtcct tcaggaggtt ttggaccagc acctaaaagt ccgttttccg
    14101 actggaagat aacactaata ccttccggaa tgtgatttgc agcttcagta ggtaagccaa
    14161 tgcccaaatt tacataatcg ccatctttga actctttagc aatgcgtgct ccgatcatag
    14221 ctcgtttatc taatgccatc ttaattctcc tttgctaaaa caagataatt tacaaaaaca
    14281 ccaggagtta ttacctcctc agggtccagt tcgccaattt ccacaatttc atcaacttcc
    14341 gcgatggtaa ttttacctgc catagccata ataggattgc tgttacgggc tgttttgcgg
    14401 taagttaagt ttcctaattt atcggcttta tgagctttaa tgagagcaaa atcgctggga
    14461 atagcggttt ccagaagata atcctttcct tccacagtga taatttgttt cccttcagcc
    14521 acaattgtcc ccagtcctgt gggagttaaa atacccccta aaccggctcc aaaagcgcga
    14581 actctttcca taagtgtccc ttgaggaaca agttcaatat caatttcgcc ggcattcatt
    14641 tgggcttgaa tggtcttatt agttcccata tgcgagacct gagcactttt aaccatctta
    14701 tttacaacaa gtttacccac acctctgtct tcccagtcgg aagcaataac gattatgtgg
    14761 aggtctttat ttccgttttc taccattgcg tcaatgattt tctccggagc tccgacagct
    14821 aaaaaaccgc caatggcaag acggtttccg ggtttaatca ttgcagctgc ctctgcggca
    14881 ctaataattt gtaccattat aactccttat atactttcta attatcttta taacatatca
    14941 aacagcaggt tctgaattgc gggactatca ctcacgggct caacaatatg agaataatta
    15001 tcaaagaaga ccttgccata ctcaggatca aactgaattc ctgcattatt aatcacttcc
    15061 tgtctggcag atttggcatc cagattttcg cggtaagctc ttttactggt catagcatca
    15121 taagcatctg caatagtaac aattctggct aaaggagaaa tttggttacc ggttaagcca
    15181 taaggatagc cctttccgtt aaaccattcg tggtgttgga gaattatttc atacacggga
    15241 gttggcaatc caatggggcg aataatttta gcaccgataa ccggatgctg ttttaccatt
    15301 tcatattcac tggaggttaa agaccctttt ttattgagaa gggtgtcgta aatgccaatt
    15361 ttgcctaaat catgaaggat agcacctatt cttaaaagtt taaggtcttc cgcatccagc
    15421 ccgataattt tacccagcat aatgcttaag acagtaactc tttcggaatg cccacgaata
    15481 taaacatcat tggcttccat tgcattaacc attgcatgaa tggtatttaa ataattcttt
    15541 tccagttgac attgggctat ataaagttcc tttgttcttt tttccaccag ttcttccaga
    15601 tttaagcgat acatttcatt ttggagaagg agggaatttt tctgtaatgc ctgtttaact
    15661 gaaatatgta aatcggataa ttcaaagggt ttatggagaa tatcaaaggc acccaactga
    15721 acagcagagc gtattttttc cgtgtctact tcacctgtta tcagaataac agggatatta
    15781 ggattcagtt tattgatata ttccaaaagc tgaaatccgg aaagattggg cattccaata
    15841 tcggaaataa tcacattgaa aatattgctt tccagcagtt ccaatgcttc ccgggaatca
    15901 ctgcaggtag ttatcctaca gggaaaatcc ttttgtaagc tcattcggat gcaatccaga
    15961 atagctaaat cgtcatctac aatcagaatt tgttttttta agttatccat attcggtgcc
    16021 ttataaaaat gatttttgcc ctgcctaaag cgcaatcggt tttaagcaat cggcatcatt
    16081 gttttcaggt ttattcactc ttctgctgac tggatacatt tccagttcaa tttccgaagg
    16141 aactgtaagc agcaattgga gttcttgggg attttgtgct gcaggattca gccaggtatc
    16201 atataatgaa ggatttagca aaaggggcat tctttcgtgc aagggctgaa taaaatcatt
    16261 ggcagaagta gtaattattc ccaaagaagg aatataactg ccgtcgggtc cataccaggc
    16321 atcataaatc ccggcaagat aaagcaaatt atcccctttt gccttaataa aaaagggttg
    16381 cttatctgtt ttacgccatt cataaaatcc attggcagga attaaacatc gcctgcgcaa
    16441 aaaagatgtc ttgaaactcg gtttttctaa gatgctttca ctgcgaacat taattagtgc
    16501 ctgttccgga atttttttgc tccaggaagg aattaaaccc caacggaaaa aacccagata
    16561 acgccaatca tctttggcaa ctacagcagc cacaatattt gttggagcta cattatagtt
    16621 caattccagc tgctctgagc tctccttaat ttttagttca tctgtcatct ttttcaggtc
    16681 ttgatattta ataacctggg caaaacgtcc acacatcctt ttctccttat tgattgacaa
    16741 gatttatgaa agccattttt taggcaataa ctttttttaa cagcttacag ctattgcttt
    16801 ttattacagg agacagaatg caaaataggg atatagacac tgtgatggaa cgcctaaaaa
    16861 aacactttta cagcgtgaaa acacccgtag ttgaccttat tcaaattaaa accgaagacc
    16921 ctttcaaagt gcttgtagct accattctta gcgccagaac taaagatgaa accacagcga
    16981 aagtagcaga aaaattattc ccaaaagtcc aaaagataga agacctggaa aagataccct
    17041 tggctgaatt ggacacactt atcactccgg ttggttttca tagagtaaaa gctaaacatt
    17101 tgaaggaatt gcctaaagtg ctgaaagaaa agtttaacgg caaaataccc gaagaaatag
    17161 acgatttatt ggaacttccc ggagttggaa gaaaaacggc aaacctggtt cgtgctgtag
    17221 cttttcaaaa acctgccatc tgtgtggatg ttcatgtgca tagaatttgt aatcgttggg
    17281 gctatattca gacaaaaaca cccttggaaa cggaaatgac cctgcgccaa aagttgccgg
    17341 aaaaatactg gctgaatttc aattcctatt tggttgcttt tggacaaaat ctgtgcactc
    17401 cgcgcaaacc caaatgtgaa atatgccctg tagcggaatt ttgcaagcgg gttggagtat
    17461 aaaaaattct ctcccagatt tcaccgatta cacagataaa acaggttagg gatgaacagg
    17521 attataggga ttttggggat gatgatattg tatggattcc tgcttacgca agaatgacaa
    17581 tggaagcagg aatgacaata aaaaacagga aggaaaagaa atcggttatg ggatttttct
    17641 aacgataacc ttattacctt caataccttc aacaacgacc ttggcaccaa gttccactgc
    17701 cttattttct acctcaatcg caggccattc ttctcctccg atttttacat aaccctttcc
    17761 ttcggcagga atctctttgg tgacaaagcc attctgccct tttaaggcat aaacattggt
    17821 atttccacct ggtttggata aaagcttttt gcccagctta cgcataaaaa gaaaagaaat
    17881 aaaactgaga accgcaaaaa taagaatctg cagaggaaca ctattaccta aaaaaggtat
    17941 caaagaaagc agagaagtta tgatagcccc tatcccaagg gaaataaaga aaaatgcagg
    18001 atcaaagatc tcaatgatga taaatccgat tcctaaaatc atccatatct gccaaggtaa
    18061 aagttgcatt tattgctttc cttcttcaga ttgttcagtg tcttcatctt cggatacagg
    18121 agttgttctt ttatctttat atatgcgttt aactttacgt tttccgtgac catatccata
    18181 gcggttataa taacgattgt aataacctcc atatcctcca taacgataag aaccacctaa
    18241 accaccagct ccgatgcctt ttactcctaa agcggcaaga aaatacatta gcaaacgagt
    18301 tccataataa gccagaattg ctcccacagc aaaaataata agccaactga agaaattgag
    18361 agctaaagat ttaatggtta ctcctatggt attggttttt tgttgtggaa ccaaagtaat
    18421 aaaaagaaga acattatttg attccttcag tttatccaaa ttggaacgcg tgctctggat
    18481 cagggaatgt aattcttctt tgcgcctgtt ttgagtatcg gtaggaaaac ggacattatt
    18541 tagctctgcc tgttctcttt cataactctg taagcggaca agttcacttt cataatcaat
    18601 attcaccaaa gcagtatcaa tttgttctgt ttgagagttc actgaaccaa aactgcgtaa
    18661 tttttctaca ataataggca ggtctttttg gggaacacta aaaatataag caccaaaaga
    18721 gccctcgctt tgctttctaa tctgtttcgt agaatgttcg ctgatgattt tttcaatttc
    18781 ggttttggct ttggctacat tcggagaaga aaatgatatt tgagctttat tgagaaattt
    18841 gaggtcccga tattcatttt gagccggctt aaaattgtag gtctcgtgtt ttccggcttt
    18901 attttgccac tggacaattg aggcagctat tgcaaggaac actataaaga tagctattcc
    18961 tctaactctg cgtttttctc ttttcttcac attaacctct tatgtttatg tcttttaata
    19021 tttcagcagc atgttccaga gctttggcag ttaaggttcc ggaaagcatt ctggctattt
    19081 cctgcaagcg ttgttcagct tccactttgc gtatattcac aaaacttttc tcaccttgag
    19141 taaatttttc tattaccaaa tgtttttcgg ctcttcctgc aatctgagcc aggtgtgtaa
    19201 tacaaagcac ctgttgcctt tcagccagtt tggcaataaa atcagctact atagcagctg
    19261 tttttccacc aattcctgat tctatttcat ccaaaataat cagtttgggc tccaagcggt
    19321 ctgccagtac ttttttaata gccagcaaaa ttctggataa ttcacctccg ctgacaactg
    19381 cagataaggg ctttaattca aaacccggat tagcggaaaa aagaaattcc acactatcct
    19441 gaccgctttc cgagacggca gataaataat cagaaattac aaaattatcc tcagcttttt
    19501 tgtcaatcat aatttctaat attccctcgg gaatacttaa ggcacggata ttctcctgca
    19561 attccttact aagttctaca gcggattttt gtcttttgcg agaaagttcc tctccttttt
    19621 cccttaatcc agcaaagttc ttttccaagt gtttttccaa ttctgcaatt gacttttcgt
    19681 tatcggcaaa agcggcaatt tgagcagacc tttcggcaaa aagattgagc agttcttcaa
    19741 tgctgcgaac cttatgttta tgcaccagag aattgatcaa atccaggcga gcttgaatat
    19801 cctgcaaatg ttgtggttca aaggaaacat tatctttgga gcgggaaaga ttgagggatg
    19861 cttcttgcag gttttccaat gcctgttgca tattgtttac ggcatatccg atttcgggat
    19921 taaggtgttc ataatgtttc agtttgctta agtaaccgct gattatatca aagacgctgt
    19981 tttcctgctc taaaagttct gcagtaattt ggttatatag ttcagttatt tccaaggtat
    20041 gggaaagcag ttcatattca ttttgcagtt ctacatcttc atttatgtgt agtttggcat
    20101 tttccagttc ttcatattga aagcgataga gttcatttat ttctttttgt ctttctgctt
    20161 cgtttttcag ttcatccagt tttttcagtc ctgctttcag ttctctgtat aaattggcaa
    20221 attcctctct aatgcttgtc aaatcagcat aagcatccaa taaatcaagt tgataggaag
    20281 gagaaagcaa acgctgttga tccctttgat ggtgaaaatc aatgagataa cttttcaatt
    20341 ctttcagtaa gcttactcct actttgcgtc catttacata atagctggat ttgccgttag
    20401 gacttatttc ccgtgccaag attaactctt cctctgtttc tattccgttt tgagcaagga
    20461 agttattcaa ttcttccgct tcttcaaggt tatagcttac ttccagatag attggctgat
    20521 ctttgtcaaa tgcttcaatg ccggctaagt tatctccgaa aataagagaa attgaacctg
    20581 ctacaataga tttaccggct cccgtttctc cactgataac ggttaagccc ttatccaaag
    20641 ataagcgcat ttctggaacc agaagataat tctttatgta aagctcggat aacattactt
    20701 gcccaaattg agtttcctgc gcaggatttg atagaaagtc cgattggaaa gcttgatgaa
    20761 ggaaacgcta cgctcggaag cagttacaaa aacctcatca ccttcttgaa tggtctgtga
    20821 attttgaccg tcaatttgta acatcgctgg ttgacttaag ccataaacct tcatcgccaa
    20881 gcgttccgtt gctggaaaaa ccataggtct gatagctaaa atatgaggat tgagtggagc
    20941 aagaacaatt gccttcattt ctggagctaa aatcggacct cctgctgcaa gtgaataggc
    21001 agttgaaccg gtaggagtag ctgttataac tccatcacaa cgggtatcaa atacatagcg
    21061 tccactggct ttaatccgaa tatgaattaa gcccggactt tccgctttat gaataacggc
    21121 atcatttaat gccaatgctt catagataat ttttccctcc cttttcagat ggcattctat
    21181 taacattcta tgcagtaaac ggtattttcc ctgtttcagg ttttcaatgg atgaagcaat
    21241 ttcaggaaga acactttcgg aaaggaaacc caaataacca aggttaattc ctaaaatggg
    21301 agctccggtt aacagagcca aatccttagc ttttaagata gttccatcac cgccaaaaac
    21361 caggatgcag tcaatgtgtt tactgtttgc cggtttaggt aagggtttaa atagctcttt
    21421 aggtaaatcg ggaatggatt ctatgctgaa gaaattgatg tcggaatcgt tccttaattt
    21481 ttccagcaag ttatagatgc tttttttgtc actgaactca ggattgatgt aaaccgcaaa
    21541 attctgcatt ataaactcgc ataaagatat tgatagctat cgtagcgttc ataaggaata
    21601 attccttctt ccatcgcttc caaaacggca cagccatctt cattaatatg acggcaatcg
    21661 ctaaatttgc atttactata caagcgttca aaaccaggga agaccttagg aatatgttct
    21721 ttatcttcac tatgcaaagt gatggtttta attcctgggg tatctaaaag atgacctcca
    21781 aaactccagg gaattaatat tgattgtgaa gtagtatgtt ttcctttttc attgcggtcg
    21841 ctaacttctg ctgtaggaag gtttaaggag ggttccaaat agtttattag cgaggtcttt
    21901 ccggttccgg aatgccctgt aaatactgaa tctttatttt tcaacaattc cttcaattcc
    21961 tgcataccgg ttccgttaac tgcagaagta caaataagag gatagcccat tgcttcataa
    22021 taagcagttt gttcttttag ttcttgttca tcttccagta ggtctatttt gttgataacg
    22081 atgatgggtt tcaatttctg gatggcaact atacaaaggt agcggtctat aagtcctgcc
    22141 ttaaatcttg gttttctcca ggaagaggta ataattacct gatctatatt ggcagccaga
    22201 ataatctctt tttgaaaact ccctgttccg taacgaatta aagcggaacg acggggcaga
    22261 attttttcaa tgcgtctatc tgaattattg gcaacatcta cggcaacaaa atctcctaca
    22321 gcagcaattg tatggctttg atacaagaac tgtttcagtc gtccgctcat tttagctgtt
    22381 accagttcac cgttaatttc tacaatatag cggtagttgc ttttaatttc cataattctg
    22441 ccctgcacca atttaagccc ttccttatag gactggtttg cttttgtgct gatgcgtgat
    22501 gccttaaatt ctgccttttc tttaatctca tccaggattt caatatctgg cagatggaca
    22561 ttttttattt tacctgtcca gcgttttgtt ttctttttat ctttttgttt catttccctc
    22621 cagggcaaaa gggggtctat ctaatcgcgg gagttatagc atcaaagagg tctgcggctt
    22681 ctttcacttt gctttccgaa ccgaaaacgg catagtgatt tttactcata accgcttcaa
    22741 tcagttcggc atagtttctc atatcttcta ttttagcgga aagcacttca tctctaattt
    22801 gctgtctatc ttcaaaagtg aaaccggtga tataatcctc atccgcttta tctcctattc
    22861 cttcaggagt taaaggaaaa tccaaagacg aaatttcacc gatgatatat ttatcaaatt
    22921 cccgcttatc acaatcaaag ttccttaaga actcgggaat ggtgttatag acctccaggg
    22981 tttctcttaa gttaggatcg cggtaggaaa caaagtataa ataaccgcca agggtaaaat
    23041 cactcatatt tccgtaagcg ccacctttca cacgaagttc ctgatacaga taactactcc
    23101 gtagaatgtt atttagaact ctaagtttac cgctgtagga aaagccctta cggaaaaagt
    23161 taccgccttt agcacagaat tgaacttgca ccggagcata aataccttcg ttgaattgcc
    23221 gcacggcaaa atgactttct acgggtgcaa atgcctcggt agaaacatta tcggcaaaag
    23281 ttcccagttt ttctatggca gaaggtatta tatcttcatc tgccgtaatg ctgataatca
    23341 tattatgggt agtaaagaag gtctttttaa tccagttcag ttcctccatt atttcatcaa
    23401 tggcagaatc catactgctc acaagatcgc agaggaaatg atagtaaccc agtccattag
    23461 ttatatctat ccaatgatat atttgagaaa ggggttttag cattctttga atggcaattg
    23521 tgtgtccttt gaagggtaac attgcttcac ttttggcttt taattctgcc agtagttttt
    23581 ttaagcgttc cggttcatca aaaagcggct ttagggcaaa atcggaagca agctccatta
    23641 gtttaccgaa tttgttttta actgctttcc cgcgtaaaag gatcttgggt aaaatatcat
    23701 ccggggtttg gtaactgtta taaagagcta tatctaaata aattccaccg gtattactat
    23761 caatttccgt agagcgtttt gcatagctgt aattttcact gttcatccat tccaccagtt
    23821 gagtgtaaag cttaatccag ggcaggtctt cttcttctgc atgggcaagg tcaaagtagg
    23881 atttgaaata gacaataccg ttggtgtttg caggatgttt cagaagttta atatcgttcc
    23941 agatgtcttc ttcggtagga tagtgttttg cctcaggatt gaggtcactt aaggaaagca
    24001 taggtatttt ttccagattt tccttggttt cgggttcctg ctgccattga atcaattttt
    24061 tgttgaattc aattagtttg gcaatttctt ttttggtcat tttctttttc agagcagcta
    24121 atttttccgc ggtttcctgt tccatttttt gaatcaaacc tgggacggga acaaaggtaa
    24181 tttggctgga gtgtttatta ttcaggattg ctttttctat aagttcttca aaatagggtt
    24241 ctttcagtcc tttgcgtagt tctttcagca ttggctcaaa ggcaagttta tctacaggat
    24301 tgccgttatg catccaaagt ccttgtgaat taagggcata aatcaagccc ctgggataaa
    24361 atctatattg agcttcccgc aagttaaatt ccattttatt gataaccgct tcaatcagtt
    24421 ttttatccag accttctttc actatccttt ttagttcctg tttgatcagt ttggtcaagg
    24481 gttcaatatt ttcctttttc acttgtttac agagcagaaa tacggttggc tgcaggatat
    24541 catcttcaac aacgattgtg ctgtctttag catagccaga atcctgaatt gcctttttta
    24601 agggtgaagc aggagtatcc atcagcattt ccgctaatgc ttttaatgtc tgcgacaggt
    24661 atttatcggt aatctgtccg taagtccagt tgagggcaag ataatattga tcctcaatat
    24721 ctttgccttc ttccacaggg atttccattt tcagcacttt ggttttgccg aagggttttt
    24781 ggagagggat ttgcactttt tcgttgttat cttcaaattg ggaaaggtat tcctcatcta
    24841 ttattttcag tgtttcctca atatccatat cgccataaag agttattttg ctgttggcag
    24901 gatgatagta tttttgatga aaagcaataa acttttcgta tgttagttcc ggtatggctt
    24961 caggatcacc tccgctttca aaaccgtagg gcgtatcagg aaactgtgct tgacgggatt
    25021 ttctattgat actgttttcg ggagaggata aagcaccctt catttcgtta taaacaacac
    25081 ccttatattt caaaggagca tcttcggagg tcaattccaa atgccatccc tcctgatgta
    25141 aaatattggg ttcattataa atattgggga aaaacaccgc atccaggtaa acccgcatca
    25201 agtttataaa atccttatta ttagtgctgg ctacaggata gcaggtcata tcggtaaagg
    25261 tcaaagcatt cacaaaagtg ttcatacttc ctttaaccag ttccataaaa gtgcttttgg
    25321 caggaaagtt tttactcccg tttaggactg agtgttccat aatatgaggg caaccggtat
    25381 tatcttctgg aactgtttta aacccgatca taaaaacctt gttatcatca tcacattcat
    25441 agtgcataag ttctgcaccg ctttgcaaat gctgatagcg ataggcagtt acctttatct
    25501 ctttaatttc tttcttgtca atcagctgga agccatagag caactttttt tgtttcattt
    25561 attacctctt atatcagttt atagtcctgc agacattgtt ttagcagagc acgattgttt
    25621 tccattagag gacacatcgg actgcgaaat gccaaagtaa tttttcccat cattgccagt
    25681 gcctctttcg cgggaatagg attggtttca ataaacatca tttcatttaa gcgagccaaa
    25741 tattgatgct gtttggctgc tgtaataaaa tcacccttca aacaggtggc aatatgttca
    25801 ctcatcagtt ttggagctac attagcagta acagaaatag ttcctttagc tccaatagca
    25861 attaaaggaa ggttcaaagc atcttcaccg cacatcacgg aaaaattttc cggagcatca
    25921 cgcacaattt gcgttacctg cactaaattt ccgctggcat ctttaatacc gacaatattg
    25981 ggataggtct ttgccaattc aacagtggta gcagcagtaa tatttactcc tgttctaccg
    26041 gggacattat aaatcacaat gggaatatca attttggaag caatgatgcc aaagtattcc
    26101 aacatacctt tttgcgtggg cttgatgtaa tagggagtaa taaccaaagc ataatcagct
    26161 cctaattctt ttgctttttg cgtggaagcc agggtctgat gtagattgtt tgttccggtg
    26221 ccaattatta caggtaactt tccgtcaatt tttttcagag caaaacgcaa aagagcatct
    26281 ttttcatcgg aagccagagc gcttgtttcg gcagttgttc ctaaaagtaa aatacctgtt
    26341 gttccattat ccagatgaaa ctgaatcagg tcttctaaag cagtccagtc aatactgcca
    26401 tttttaaagg gtgtaatgag ggcaacataa gttccttgca gcattttttt aacctccagg
    26461 gttatatatc cttatctata aacatatttt attccataat agcaaaagag acgattttcg
    26521 tcaatcaaaa aagatgaaaa ggttattttt ataagcaagt tgcctcccca aatagctttt
    26581 tattgcttct cctgctttta cctaaagaaa aaataagaaa acccaaaaga caaccagcaa
    26641 aatagcaaaa aaggtcagta taacgaaaac caaaacctaa aagtaaacca ccgataatag
    26701 tggaacggat tttttccagc catacaggat gccacaattg gctaagttca attaagcagg
    26761 aaaaatcaaa ggttaacaaa gcaatctccc aattcctttt acgggaaaaa agcatccgga
    26821 ataaagcata aaaagctaat gcccacattg tgtcaccggt atattctgca ataaaattcg
    26881 gcagataatt tccatattta cgcgagccaa ttcccaaaat tgcgcttaac acaataacca
    26941 caaaaggaaa tccctttttc ataatctctg gcttttttct atccacctgc tgtattgcct
    27001 tttttcttta tctaatttca aataatatct ttgccataat ctatacaaaa gattttaagt
    27061 caagcacttt gtagacggag cttgctaaaa gggcagaagc caatagggaa atgaagaatg
    27121 aaatttagaa ggaaaacaga gagtaattga gaaggttgag agggttgaga aggtttagaa
    27181 ggtttagaag gttgagaagg ttgagaaggt tgagagggtc gagatgcatt aaaagctcgg
    27241 agagcgaaac aatgatagcg atggtggcgt aagcccctcg taaaaatgaa agccccgagt
    27301 tttttttctt ttttcctttc ctttttacaa aagcccgtca gggcgacaca atgatgtaaa
    27361 aggtggcgta agcccctcat aaaaaaaagg ttgagagggt ttagagggtt tagaaggttg
    27421 agatacatta aaagctcgga gagcgacaca atgatagcga tggtggcgta agcccctcgt
    27481 aaaaatgaaa accccgagtt ttttcttttt tcctttcctt tttacaaaag cccgtcaggg
    27541 tgacatagcg ctgagagcag agggcagaga acaaaagcat aaattccgtt agaaaccttt
    27601 tattgctgtg gcaaagaaag aatttgccaa ccctttttcc cgtagcagta ttctttggct
    27661 ttaagaatgt tccgctattt ttaaaaacac ctcacccaaa tacccgtatc taagttatat
    27721 tgtctaaaat tctgcgttga ggagttcata ggacttaaca agaggtaaca aacctgcttc
    27781 tactgcctgt ttcacgcttt tgcagggaag gttttggcta tagatttggt cccttgtaag
    27841 agtgccgaga tttaccgtct cgggaaacgc cttttgcaaa gcgaacatca atgttgtgta
    27901 agcatttgtc caagtgtgca agggcttttt tccctccggc agattagcgt tgaacaaggc
    27961 aatatatatt ttcaaatttt gcttataacc ctctgaaggg gaaagtgcat aaatgttttg
    28021 gatatttttt gtaaataccg gatgagcggg atgatttttg aatttaaagc gacggcgcac
    28081 ataaagtttg ccgtttttct tgctccggta atagataagt ccatccaagt gaccagtaaa
    28141 ttcacccaga agattggcgc gtgttgcttt catttctttt ctccttatag catttttttg
    28201 ggataagtta ttggtcattc ctgtgcaagc gagaatcagc ataaaataaa aagtatgtat
    28261 gctttttaaa tctgctattt atattgtttg tattattttc cgcatctcgc acagcagaat
    28321 tcggtagacc aattatcttg ctcccataat aatataaggt gacaaaaagt tattctcggt
    28381 gacaaaaatg ggatttattt cctacctgaa ttgctgaagc attgcttacc cattacggat
    28441 ggcattagca atgggtaagc aatgggtaag caatgcaatc aaggaagaag atgtaattat
    28501 atttatttag taggagttag gaaaattccg gagaataatg aactattgta ttggtaagtt
    28561 catattttat tagaggttga caaggatttc aaccttccgg tgtttaatga ctctcaaact
    28621 tttttactca atcggaaagg cattgatgaa accggtaaaa ttcagggttg ccagcaaagt
    28681 gccaatcaac aatacaatta taattgtagc tttaaaataa tccgatgcct tcagcatact
    28741 aaccaagtcc ggattgcggg aaatataggc agaagcagca taaaattctt ctccaatcaa
    28801 ggtgtaagaa caggtagtaa taaagaacgg cacttgggtt atagcatcgg ttccggcaat
    28861 ctgaaaagcg cctgtttggt ttcctgtttc cgttaaaagc agtgcttcag cattaaaata
    28921 tcccatataa aaaatagtgg caactctttc cctcacggtt attccattga ttcctgcaca
    28981 gtaaggaaac tgatcataag aaatgaagaa aatatcattg gaattaaagg catccggacg
    29041 tccaacttca ttatacgcat tttgaataat ttcctgggct aaaggcaaaa ccatatagtc
    29101 acaatgaggt gaaattaaac ggacatcaaa ttcggcggct ttttttgcta cctgccctaa
    29161 aatcaacatt gaagcaaccg tatcgggatc acctaaagtt ccccaaccgg gaacaaacat
    29221 tataggacgc cccatttctg tagctctgcc aactgcttct tcaatagatt ccaaaccggc
    29281 aatgggtctg atataagcat ttcctttccg actttgaatg atggcataaa taaggaataa
    29341 acacaataaa atagaagcag caagagtaat atatttggta gtatcaaacc aatcgctccg
    29401 cggataaaat atggtattcc ctttagcatc agcataaatg gcagtagtta caaaattggc
    29461 ttttccatct gttttaataa ccttgtagga ataactgcgc atatttttat tatgcgtagc
    29521 taataaggtc ttcagccatt ccttattggt ctttgccttt tgagcttgca gataagcagg
    29581 atgcttcgtt ataaagttat aatttccttc cgcttcctga attgccgcca ataaagaatc
    29641 gggaatagca ttagcaggat atttgttttt ttccgccaat aaattatcca gttcctcttt
    29701 ttgttttttc aggtcttgtt caaatttatt acggaataag ggaacagcaa aagtatatcc
    29761 gtttacctga tcaacagcta ttgttatttg cgggtctaaa atcagacgct tacccttgtt
    29821 atcataatcc agaatgcttt tatacacaat actttcgtag ggaatggaat ggtcataagc
    29881 ccgggttaag ccattagtcc tctctccaaa caaaaaatca ggatcaaaac cattgcgggt
    29941 aagaatttca taatagagct tattcaccgg ttcattctga tagtagatat tttcgcccct
    30001 aaaaatttta ttcccttcat cataaacaat atgctgttct gtataatctt caaagctacc
    30061 gttgccgtta gtttgaacgg caatgcggat tttcatattt ttaatagcga tatactgttg
    30121 cggtagtttg aaatgaattg ttttatccaa atagtattcg gagaccttgc caatttcttt
    30181 atttaaggaa tcaagaacgg taaacctcaa ttccttaatt ttataatgtt cattattgga
    30241 aatttcgtaa tttatccgta aagttgtctt tgccttattc aaataaagca cttggctgac
    30301 aaaaaccagg ggttttccca tagaaacgga taaataatct cctttgtcgt tgggtttatc
    30361 tttaacctca atttggggta ttttaagcag ttgtgaagaa ttcaggatac gaaaattttt
    30421 agctgaaacg gctgcacttt gacccatata gtcgctgtaa gaaagcacag gataataatc
    30481 agccaaattg gcaggtaaat ttatcccttc ttctttggta tctacactat aacaggtatt
    30541 tacggctccg gaataattgg gaatctggaa aaggggaata gcgctatttt gccaattttc
    30601 agaaagtaat tcaccattat tcggtttttg cgctaaagga atcagccaac cctcccacat
    30661 tgcaatatct tccgaagaag atggtggtag ccattcaaaa ttgaaaactc ccgtatcttg
    30721 gatgtaagtg ctggaaagat ttgcggatgg ttctggcgga ttatcttcag gaattacttt
    30781 ttgaatatcc gcataaggca aatattttcc tctttcgttc actgccagaa ctgtgtagta
    30841 atattcctga cccgctttgg gaaaacctaa aatggtttca aaccggttta atttcaagcc
    30901 cgccataata tcttcatctt ttgtcaccgg tttggaacta tgataaagct tggaattatt
    30961 gatggctcct attatattgt atctggtcag cattgtagca agcaattccg gctgttgagg
    31021 aaactttttg aaaagtggac tattatggct ttgctgtttt tcctttttca gcttttgggg
    31081 agccgtttca aattctataa agggttcttc tcccttatcg tagaaatata attccggtga
    31141 caaaacacct aatttgggat caacttctat gctgccaata taaaacaggg aatcgggact
    31201 aacgcctcta tagatattat aattaataat gcgatacgat ttatccagag gtttccattt
    31261 caatacaatt ccgctaccat cgtcaaaagg catatcggaa gcggaaaatt ctgttatttt
    31321 gcctttcagg gaagcaggtt ttgcatcatc cgtggaaagc aatgaaatcc ctatcaacag
    31381 aataattccg attacaataa gatatttttt catttttata tccctaaacc aaggttccct
    31441 tatttctctt cccacaatac cagtaactta aatctataca actgttatgc taaaagggaa
    31501 cttaaaggtt cctaaattga gctatttcat caatccaaaa acatctatta ccaggaattg
    31561 aattcttcca atcagcaagg aaatacgcgc cataaccgtt aaaccaaaag aggcaccaaa
    31621 accaatcatc atataccatt ttcccaaccc gacaaatttt ccgtaagcac cggtatgtgc
    31681 cttggagaaa aagaagtata acagaacggc aattgtgcct aaaagaataa gaataagatt
    31741 gatattttcc aggggaacca tcgcctgacg catctgcact aaaattccgg catgaattcc
    31801 catcggaaca ccaaaaccga ttccaatcat agaaaaggca ataggataac gggaaagcca
    31861 acccagtttg cgcgaaaaac ggaacagata aagtattccc atcaggcagg gtaaaataag
    31921 gacaaaatta tgttttagaa taatcggctg ccacacatac ggaataaata tccgttcata
    31981 agtaaacact aaagtataac ccagggaaac tcccaccaga agttgttccg ccattctata
    32041 aaagggattt tccttataaa ggaaggagaa aatacacagg gttaaaaatg ctgctacgag
    32101 attactaaat aattcaaaac tcattgttat cctccctttc ttacttttct gccgttttgg
    32161 ctttctgaat gaaataaccg atattaccca aaaggataaa gatgatgatc ataatatggg
    32221 caaccgattg agcattcatt ccaattctgg ctactttgaa tttataactg atattatgtg
    32281 taagccgatt gatttcccgg taagcatttt ctcccatctg atttctcagt tccggagtta
    32341 tcttcgttac atcaataatt attgtgccgt tttgttcttc tttcaattgg tcaaaaattg
    32401 ctttctgctc cggatatttg gctacaaatg cagtatattc ctcgggagta aattccgcct
    32461 tcgtttgcgt ggtaatattg atcaatttct ttgtgctctc atcatttaga acttccttgc
    32521 caaagggacg ccctggcaga atcttgtttc cttccgcatc tgttttaaca ctatagtcaa
    32581 ttgccggatc acgataagca gcaaaaacat caaccagctt ttcatattcc gctgcaccct
    32641 taagtcctgt cagcatacct atcaattgac ccgattgcaa atagggatat tcatcggcag
    32701 ccataactgc tgtaactccc aaagcaacat taactccaaa tttgggtcgg gcataatata
    32761 tccagtaaac tcctggactg ctaccggaaa actctactac caggttcatt tcgttgtaat
    32821 tggttattcc tttcataatc ggtaagcttt ccagtttacg cccttcagca tcagtattta
    32881 cagcattggc aatattatct cccattccca aaactacagc cattggctga ggtttatagc
    32941 caaaattgca atagtccacc ccgcttttta tatcaggata ttcttctttt accgaattga
    33001 aagcataatc aataatagga gctcccgaag ttaaaaaagt aagagcaaaa acctttatat
    33061 ttctttcaaa acaatgccgg ataatagcta tttccatagg ataaagttca ggcatcgtag
    33121 cagcatcgtg taaaaaactt aaaaggacag ctctatcttc tctgccggca aaggaatcaa
    33181 tcatctgata caagttctct gtaggcggag aagttacatt atctgaattg tagggtataa
    33241 ttaacgggat gataattgcc agcgctacta taatgtagat ccagcgccgg tctaaacttt
    33301 gcattctttc aaaaaagttc attcttcatc ctccctttgc ttaatccgtt ccacccaaat
    33361 aggaacgctc aatacctaaa agaattttca gagaagttgc tacaccacca agtccaacac
    33421 cgatggcaat tcctcttttg gaagcaagat tagggacatc caaaatccat tgtgatataa
    33481 ttggcagatt cttataaatc ttaactccca aaggcacctg cgctaacata actacaattg
    33541 cggcaagaag caaaacggta gcttccacgg aacgcgctct aaatgcctta taagcagctg
    33601 aagccatata aaatgccaaa agggaaaaca ttgtggaact cattggcatc tgcatattct
    33661 caaaaatcca catcaaaaca ctaccttttt gagttccgcc gataaaacca gcaagggaag
    33721 taataataaa cccggcaaaa aagaaataac tgtattgcca tttgggagct tttcttttaa
    33781 ttttactgct gtgcatcata cataaactga taactcccaa aagagcagcc caaggagaag
    33841 atgcataaac ccagggaaga tagaacttgt tataaaagtc atcctgtaaa agcttatggg
    33901 gacttaaggc ataaagcaca aagatgatgc cccccacgat tacgatgatt aaaggtattg
    33961 tcttcttcat ttagaacctc atttatttta ggggaaataa ttcactaaga ccagtcaact
    34021 ggaaagtgga aagaaccgct ccagcaatta caaaggcaaa aatcaggaat ttgtaatagt
    34081 cctgcgcttt caaagttccc agttgcattg gttcacggtt taaataggaa gaagcggcat
    34141 aaagctcttc tccaattaaa gtatagtcac aggttgttat aaaaaaggga atctgcgtga
    34201 tggcatcagt tccggctatt tgataagcac caataccatt acccgtttca gtcatcaata
    34261 atgcttctgc agcaaaataa cccatataga aattagttgc agctctttcc cgaatcataa
    34321 ttccattcac accagctaca taagcaaact ggacatcggt taaaaagaat acactgtttt
    34381 tatcataact atccggacga cctacggaat aatgtgcatc tctaacaatt tcctgaacta
    34441 ccggcataac gatataatta taaacgggca caataagttt aatatcatat tctgcagctt
    34501 ttttggctac caaacttaaa attcccatag aggcaatagt agctacatct cctaaagaac
    34561 catatcccat catatacata atcggacgtc ccatttccgt tgctctacca actgctgtat
    34621 caatttcttc caaaccggca attggcctta tatacaagtc cttaccacgc ttggctaaat
    34681 tgataaaaat aactaccatt aagccgaaaa taatcattgc cacgagggta gtatatttat
    34741 tggtatcaaa aacattggaa acgggcttca tatagaatat atcttctccg tcttttaaca
    34801 ggtcactttc cgtaaaaagc cctctgctat tagtacgaac aacctgataa gtgctgtatc
    34861 gggaatagtc ctctcgtact ttagcaatat aacgcattct gcttttccgg tttgtaattt
    34921 tattggcatt aaccagatgt tcatttttac tgtaagcatt taactgcttt tgcaattgct
    34981 ctatattttg atttaactgt tcaactacag caggatcggt ctcaccggtt atcttgtctt
    35041 ttagctctgc aattttttta gttagctcct ccaccttttc ctgtgcttct tttctgccta
    35101 aatcggggaa aatattggtc tcaatgcgcg tttcattttc tttgtcataa accaaatcaa
    35161 tattgggaga aattaaagct aaaggtgttt ttatatctcc cggctttaat ttacgcgctt
    35221 gcctctctcc attgatatag gttatcaaag tatcaccctg ggcaaaagta aaaccgttaa
    35281 ccggcttttg aatcatagta gcataaggaa ttgtaacatc taaagaatta tcataagagg
    35341 tattgcgctt aaccaaagtt aactgcttgg aagccatatt tttgcggtaa actacattgc
    35401 tgatcttaga tacatctttg ccattacgat aaagtgcctt ggtaggtaaa atagccagca
    35461 tattggggtc atattccaaa tcctgctcta tactatagtc cttaggtatt tctggcttac
    35521 ctgccaaagt gatttttacc cgaaatccct ttttgtaatc gtaattattg ggaactttta
    35581 agataacact gttatcctga taaaactcgt taattttggc aaagggtttt tcctcaccct
    35641 tcttatagaa ctcaaaatag atgttatcca cttgctgagt ttcggtttta tttaactgat
    35701 aatttaccct gaatttattg aatttgttgt tcagagctgt ggtcttaact acaaaacaaa
    35761 taggatgatc ccaaacaaca gttaagcggt cacctttgtc attgggctta tcttccattt
    35821 ttattatcgg tttggcagga aggtctttac tatgcgttat cctgggttta gaaagagcgg
    35881 aaaaactgct gaaaccatcc gcatctatta attccaaagt ataagctgaa ttaatatatg
    35941 cctccagaga cgcctcttca ggaatagaaa cctggctata atttttcccg gaaccataac
    36001 ctactaaacc gctttgcaga actactgttt tggtctgaaa caattcagga tgtaagcgaa
    36061 gagaatccca ctccgtaggt gtaaaaagag gtgtttgatg aatacgatat tgagctatgt
    36121 ccgaattgta taatggaagt tcccattcaa accgtaattc cctgctatct tctaaaacca
    36181 cactgtgtaa tgctgttgcc ggttctggag cattaggttc agggctacct gctgtaattt
    36241 tagcgtgctt ttgaaactgc cttctttcat tcaccgctac aatggtataa tagtatttct
    36301 cgccgggatt taagaaacaa tacactgtct gttgattgtc tttcaatccc gcataggttt
    36361 ctccctcttc ctgtttttca ttttctgaac cttgttcttg gggtttatcc aatgtaactt
    36421 tctgactgct gtaatagtat ttgttctttt cgctaacgga aagcagagaa aatttaccgg
    36481 atatttctgc taaaaaagga atgttacgag gcattttttg atataagggg ctatttttac
    36541 cctgctgctt ttctttcttt aacttgcgag gcgaagaaat atcaaaaaat tcactgccag
    36601 aattatcata atagaacatc ctttcagaag caacacctgt ttttacatta accggaatgg
    36661 catctaaaaa gaaaagggta tcaggactta ctcccctgta aatgcggtat tcaataattc
    36721 ttttgtcccg ctccaagggc ttccacgaaa gcattaatcc tgttccgtca tctgcaggaa
    36781 tatcttctac tttgaaatcc tctactacat atctttctgc gtctgactgc ttctgtgcaa
    36841 ttatcactgt gcttaacaat aggagcagaa acagcaagag aaagattagg cgacttctga
    36901 ataatctcat acgcttggtc tcctgtttct ctttcttaac agttatttaa accttctgtc
    36961 ccacgcatag aaaatgccca tttatgtcaa tctctttttt tgctatgctg cagctcctga
    37021 tgtcttggag tttaaaaccg tctacattat tgtatcgccc ttagttgttt ttttgaacac
    37081 aagaaagaat gagacactga ctattatttc tttctttttt caaggggctt atgcacccat
    37141 cgctatcatt gttaagccct ttggggcttt tatatctcgc tattttatcg ctacatacac
    37201 cggtatatct tgcacccaaa gtttatgaat agcttcggga cccagttctt gccacaggaa
    37261 tgtttcacag gaaacaatat gttgtgaaag ataggcagaa atgcctccag gcataacaaa
    37321 atatatcgct ccaaagtcca taattgcttt ttggacttct atagagcgtt cacctttgcc
    37381 aatcataact ctcaagccgt tttgaattaa aagaggggta taaatatcca ttctggaact
    37441 ggtggtaggt cctattgctc cacaaatatt tcctggtctg gcagggcttg gtccgcagta
    37501 aaaaagtgtt gtttctgcca ggtttaaagg tagtttttca cctcttttca gaatatccac
    37561 cattctttta tgggctacat ctctggctgt aaacagacag ccggtaagca aaaccttatc
    37621 agaaatatgc agttgagcaa tatcagcagg acaaagaggc aaggtaaatc tatatgtttt
    37681 cataagttag ataactattt ctatatgccg gtgggaatgg cattgtaaat ttactgctac
    37741 cggtaaggaa gcaatatgac agggagcgtt taaaatatgc acagctaagg cagttgtttt
    37801 tcctcctaaa ccttgcaccc ctattcctgt ttcattaacg cctttcagga tttcctgttc
    37861 taaaagggca tatcgttcaa ttggattttt agcggtcaga ggttgaaaca atgccttttt
    37921 agctaacaga gcacaggtct caaaattgcc tccgattcct attccaatga ttaaaggtgg
    37981 acaggcattt cctttagcca gagtaactgt ctccactaca aagttcttaa tctcatcagc
    38041 tttagcagaa ggagtaagca tctttagttg gctcatattt tctgcaccac cgcctttttg
    38101 agcaatctga agttttagtt tatctccttc tactatagaa atatgaatta cggcaggggc
    38161 attatttccc gtattttctc tttgcaaaag ggggtcttta agtattgacg gacgcaaata
    38221 gagttctgca gagcttttaa ttagggtttc gttaataatt tcaggtaagg gaggtccgtt
    38281 tataatgcaa tgattaccta tttccgcaaa aacaaccaga gaaccggtat cttgacataa
    38341 aggaatttct attttaggtg ccaattctat atttttcaac atacattgaa ggatatcttt
    38401 acttatttcg cttgtttcat tggcaagggc atttttcagc aaggcgggta attcaggatc
    38461 agggttaaag gttatatatt ttaaggcgtt agcaagttca gaccgaattg tttcatgtga
    38521 aacatatctg taagccattg tttaaccctt ctgtcgttta attagatact tcagcaggag
    38581 gtcttcaata ggagtatagg tatctacggg attgtattct atttgatttt tgtagcattc
    38641 tctttttaga tattccgcga actgatgaaa attttctaca tattccttcc gtatttgcca
    38701 gggtgagaca gttatttttt ctcctgtttc gctatctata aattgtgctt ctcttttgta
    38761 atcaaacttt tcttcctttg ggtcaattat gtgaaaaacg agtacttcat gttttcgggc
    38821 acggaaatgt ttcaatgcct ccataattct ttccggttca tccaaaaggt cactgataat
    38881 gattatcagg gagcgttttt tgacgagttc agcaatttga tgcaagggag ttaataggtc
    38941 tgtttggtct tccgctttca aatccaaaag agcggcaaat aactgagaag tataacttct
    39001 aatagctttt gggggatagg cggaagtaat tttattgtta aatgtataaa gtccggcagc
    39061 atccttttgg gaaatcatca gccacgctaa agcacctgcc aattgagttg catattcaat
    39121 tttagtgctt ttgccagaac tgaagaacat tgatttactg tggtctaaga gaatgtaact
    39181 gcgtagattg gtttcttctt catagcgttt tacatagtag cgttcagttt ttgccaaaat
    39241 tttccagtcc aggtctttta acggttctcc gggattatat tggcggtgat cagaaaattc
    39301 tgcactgaaa ccatgatagg gggatttatg caagcccact aaaaagccct caactatgct
    39361 tttagcagtt agctggaact tattcaggcg aactaatgct tcttcggata aaaggggcat
    39421 cttatttctt tttagggtga tagagcaagg caaaaggaac gattacggca taagcaataa
    39481 ccaataaaat tggggatata gtaatctcat tgaaggacat aattatgtag ccaacaacta
    39541 atacaattgc cgctaccagc aacaggataa aattggtgcg gtttaattgc agtttgtttt
    39601 tttcatcgtc tttattgttc atctgtgctc cttcgctgca gaaagcaatg atttttcatt
    39661 taagaaccat agataaagaa ttgctttgtt tgtcaactga actttcttgt tccaagtagg
    39721 aggaaagaat ttgcccatag acctgattag caagttcttg tctggttttc ccttcctgat
    39781 agggtataat tgttaaaata tcaatttgcg cctcaagtga atgtcctata atttttagat
    39841 aatgagtaag gaaaggcata tctccatacc agtaaataag gtcgcgattt tttttatcta
    39901 tctttttgcc atctattgcc ttatatttaa tacaaacggg caatatagga cattttgctt
    39961 ctattgctac ttgaaaaagg gaccggcgaa agggctgaac tgttatacca ttggtgcttg
    40021 ttccttcagg gaaaagcaca actttaaagc cctgatgaat agcagaagca aatttctcaa
    40081 tttcagcagg caaggatata tatctttttc ggtttgtata caggcatcca ccgcttttgg
    40141 ttattctacc caaaaaggga ttttttctca tttccacaga ggttataaag acaaaattct
    40201 caaccgcaga aagcaatata atatcaaggt aggttgtatg attggaaaca gcaagataag
    40261 gaatatcctt caatttttgt aaattttcgg agtgatttat agttaattta atgttaaaag
    40321 catgaataaa gcgtttgctg atgcgagtgt tatttttcaa tagccgtttt ctgcgtaata
    40381 cgggatcagc actaaaaatc ctaattgcgg cagaaacaag gaaaaattca cttatcagat
    40441 aaatgtgcca gagaatacac tttattttgc gtaatatcat agtttatgca ggtgctaagt
    40501 tatgattttt tttcccagac aaaatgatta actctctgca tccagctaat tttctgaaaa
    40561 gatgatgtta tcattttttg ccgtttcgtc tttgttaatc cttttttcat acctgctttt
    40621 ctctacctat aacaaatttt ttataattct ttaacagagc taatatagtg ttaaagagcc
    40681 ataaactaat ctattaagaa acagataatt acatatctaa tattcttatg ttcaggtttc
    40741 ttttactgcc tataaagaat aatcactata tcattcttgc ataggtagaa tgtactttgt
    40801 ccttgctgag aaagcaggta tgacagagga cttttccttt aggaaataat attactatct
    40861 atctctttaa ccttctcgcc atcttaccct ctttacttga agagcaacat tttttttgtg
    40921 atgttctttt ccggggtaac aattctataa tagtatacac cgctggagca atacctgccg
    40981 ttatcatctt tgccattcca ggtaaattga tagttaccac ttttcagttc tgacccgatt
    41041 aaatctttca ctttttgtcc tttaatattg taaatggcaa tagaaaccgg tgctgattta
    41101 gtaagattaa aactgataga agaagattga gaagcaggat tgggatacac acttaccgat
    41161 tggattacag gaaccattat tggatcgtta ttccctactg gattatagtc cataacattc
    41221 agttccgaaa ggataatacc aatcatattg tttagggtat attcccctga ttctatttgt
    41281 gtagttctaa aagaggaagt aatcacagag tagttatctg tagcattcaa aatgcctata
    41341 actggattat tagtattacc gctggaggca aaaaatattc cctgagcagt tgtttgagca
    41401 ggaattatgg gatatacaga ggaatcagca ccgttataat tccaagccaa gttgctggaa
    41461 ggatagaaga tagatgtaat attaagagga ttattatctg ccatctgaac acccattttg
    41521 gtaaataact caccgtaact atcatcaata taattttcgc agtagagttt acctcctgct
    41581 tccagatagt tgtttaagta actatattca taagaagttg gctcaagttc cgtttccgga
    41641 agagaatttc ctgccaagta aaacactgcc tcaaagttat tcagataatc ataatccgga
    41701 atcagtttat cggaataaat cacattaaaa ttacttattt tcctgagagc attaacaact
    41761 gcaagtcctt catttccttc cggttcccaa actaaaattt cacctgcagg gttaatttga
    41821 ttgggagtcc agcccaccat agggtctccg aaaagcatat aagtataaac attttgtaat
    41881 tcgggataga ttattcctcc attatcaaca ggatcaccaa aaaggtaata ttgtgtatgc
    41941 aaaagatttg cccaagaatg agcagaccct aagtccagtt ttgcctgacg gaagttttcc
    42001 acaaaatgat agtcataact ggcaagccct ccccaacctg gattttgcca tccgacttta
    42061 taccaaccgg ttctggtagc ggcaataact cccaccgctt ttttaattaa ggcatattct
    42121 gctaggcaat aattattgga atccagagct ccgttatagc aggatgcagc atagataatt
    42181 gtcccgctgg tattttccaa attatcaaaa ctttggcggt taactaaatt catccattgc
    42241 atttcatttt catcaggaac attattttgg ttataatcgt taatccaaac tttgcgtgaa
    42301 gagtttgtag aactgccatg tgcatcccaa ttgataaaac cccagctttc agaattaagc
    42361 ttatttatta aattatcata atttaccggt aagtcacagg gaaaagaaag aacaacccct
    42421 tcctgttcat acatagtaaa gttttcctgt tctgctaaac aggtattccg cataaattcc
    42481 ataaaaaggg ctccgtcggt ttgtaaatat actggttccg gttccccttg ataattcaag
    42541 aaagctgcgg gtaacaagtt tttttctttc cagggttcag ttgactgttc ataggcaact
    42601 atccgacttg caatagcgga aacatttgta ggattatttg tagaaatgcg tcccacaaag
    42661 acctccgggg taaagtcaat tccataatcc tcattcatat aaccggtaga atattcaccc
    42721 cttctgccat cattatcggt atcccaatta ctgcttaaat ccgcataaaa gaaatcggta
    42781 ggaactgttt cctgcccatt tggctcagga gttacataag ccacgggaac cgtatcatag
    42841 tcaccaacta aaagcaaata ggaaaagggg gttgaggcat aagtattctg cagataattt
    42901 ctcaattttt cagcattatc ggttcccgtt ccggaagcca aagctaaagc tatatcgcaa
    42961 aaggaaacat ctacaccttg ggactggcga aatgtaacaa gccaatccaa agctgtatac
    43021 aattcgggag tagtaataac taaaagacgg ttattgccct ctttggaggt tgtataccat
    43081 tgggaaagat tttgcggatt agcaaaaaaa cctgcatcgg tataagttgg aggaataatg
    43141 cctttagctt tggcagaaga agtgtaatta atagttatct gacaggatgg actccacagc
    43201 caatgggaac cgttatatat agcaggtaat acattgaaac aagcataatt aagttcaccc
    43261 catttcttta aacccatata cctgtatcgg gagtaaatat ttctattaac aggagatgtt
    43321 aatatttcct caccattgga aaaagcagaa tttatagccg gtggctcttc tgtaaaagtt
    43381 gcttctgcct ctaaaattac ctgccaatta tctacaacgg catctttagg cagaaggatg
    43441 tttatttgtt taacgggcaa ttgtagagtt ccgggagcaa tggaaaatcc ataaccttgt
    43501 ttaaataatg cagattgaga taagccctct tgcggaacag gaaactgaat tgttgtcttg
    43561 agagttgttg ctaaaataag caacggcaaa catagtaaca caagacataa ggttcttttc
    43621 atttttcttc ctttataata tctttaagta tcagggttgc aaggtcttga ggcgttggaa
    43681 caggaattgt ttagttcaga attttttttg ttctcttttt tttccgaaga ggaattcggc
    43741 aattcataac ccaaaccctt acctgtaact aatacattaa accgattaaa catttgcctt
    43801 aagcggtaga gcatattttt atcgggatta agttgtgcca gcattttagc cagaaatgca
    43861 tcccaattta tagtttccct tctttggaaa aaaccgcatt ggcgcataac ttccaaaata
    43921 tagttgcgga tttcatccag ttcttttccg ctggcagaat tatagtattt ttcccctttt
    43981 tgtttttctt cccaaggaag ggaataaatt tcccaaagtg ctaaagcaac tgcctgagcc
    44041 aaattcagag aaggaaagga aggattagcc ataaagtgac agcgcagagg gcaaagttct
    44101 gcctcagaat cagttaaacc ataagtttca cgtccaaaaa caagtcctat cttggaatcg
    44161 ggtaaactat gaacatattt tgccatttgt ccgggggtaa aatcaacggg tttattttta
    44221 cccaggcgac gggaaaaggc aataactcta tccaatccgt taattgcttc ttccagggtg
    44281 ctatatgtat ttacattttc cagaatttct tcgctatgca tagccaggta aaaatcgttt
    44341 ttttcaggaa ctttacctac aatacgcagg tctgaaaaac agaaattatg cattattctg
    44401 gctatggcac ctacatttcc tccatagatt ggttccacca aaattactgc aatgcgtgca
    44461 aaatcctgcc taatgttcat atttgtctgg aataataatt gttttcggaa ttagctgcct
    44521 tctggttttg attttctctt ttctttttct tggctataat gcctgaaagt tttgctccca
    44581 ttgtggcaac tttaggatcg ggatcgtttc gccattcggc aatagttttt tgtgtaacat
    44641 tcataaattc gttggtttta tcccgacgac tgcgctgcgc ataaatatta gcaagttttt
    44701 tcattgtttg ccagagaatg cggatccttg tgtcatcgcc ttcaatcagc cagcgccgaa
    44761 tgctattata acattgtaaa ggtttttgtc ttcctacctg agtgagaata tgacaaactt
    44821 ttttctgaac ttcttcactt tcatcatcca aaagattaga aacgaaggaa agcacatatt
    44881 gaggatggcg ggaggcaata ttttccatcc cgtgcatact gatagcgcgt tttttttcat
    44941 tatccgattc agcccaggca ggcatatatt cctgcattac attgggagct tttttccaaa
    45001 gttcaaaaca gattgtttcc gattcccaag gcacgctttc aaaggtcttt tccactgttt
    45061 taataatgat ttcgggatga tcctgatgtt tgtaataaaa atagaagagt gcggttgccc
    45121 ttacaccata tataaaatgg tctaaaaggc gtccacaaat tgcatccatt tctccattac
    45181 cggaatattg cgctaagcgt ttgcctaatt cttctcggac aaagtaattt gccgtattac
    45241 ccaaatcagt aatttgtttt tctgcgagtg cgtctttacc tttttctata ttgcggaaaa
    45301 ttttttccac ttcggtatcc aggattttat aatcatccag attcagacgt ccaacttctt
    45361 ttatcattta ccctgctccg taacggatat ttttgttccg tcactaaatt aaatagtgtt
    45421 tttttttgtc acttgtaaat tcatcttgtt tgacctaaac taattgatac ccttccctta
    45481 tttacccttt atttaaggac cgggagataa atgggattga agtgtatcta cagctgatac
    45541 ccttttatta taagagagct atctgtcaag gaaaaagtca ggagtaatta gcgtcttcaa
    45601 aactttgacc atagaaataa ctttaaaagc gcttatgacg acgacataga ttattataaa
    45661 ttaatttggt gttctcggtg gataaaaatt tatgtgctgc tatttttttc tctgttttcc
    45721 cagagttctt ttgccacggg agcccatttt tccgcagcgg gttttgtttt agcgcacagt
    45781 tcttctatac tttcgggagc ttcaatatct gtagatttgg cattggcttc atttacaatt
    45841 tccataatct tttccgggtt atccagcatc gggcagggtt gtaaaaggtt ttggttgaag
    45901 gggtgtcttt tttggtatgc tttaaagatt ggtgattgca atgcttccaa aagagagacc
    45961 tctttaatat ttacattgga gtaatgaaca aaggcacagg gttcaacttc tccggcagca
    46021 ttgatatgaa aatagctgcg tccaccggca atgcaaccat tagcaagttc tccatcattc
    46081 caaaaatcca aagccaggat gggcttatga gttctgattt cattaaccct tttatacata
    46141 taggcgcgct gttcaggagt agcaatcaaa tcgggctggg catttttgcc gataggaatg
    46201 taggtaaagt tccaggcata gaaacagccc cttgcaatca tatcgtctac aaattcatcg
    46261 gaagcaacgc tttctgtatt atagcgatgg tagcaagtgg aataaccgaa aaataaacgg
    46321 tattttttca ttagttccat tgcttctatc actcttttgt agcttccttt ccctctgcgc
    46381 atatcggttg ctggttcatt tccttcaatg gaaaagcaaa gtgttaaatt tcctgcattt
    46441 agaacctctt cgcagaattt ttcatccacc agtgtgccat tggtaaaaga caggaagaaa
    46501 caatcctgat gttttttgca gagtttaata atgtcgtttt tgcggattaa gggttcgcct
    46561 cccgaaaaaa gataccagta aatgcccatt ttcttgcctt gcttgcagat agaatccata
    46621 acctcaaaag gcagattatg ctgttttcca tatagagcag cccaacaacc tgtgcaggaa
    46681 agattacagg cacttgtagg gtccatcaga atagcccagg gcacttgtat tgaatatttt
    46741 gctttatact tctcccgtat cggtctacca accagtgttg tattgattgc aaaacattct
    46801 gccagtttat gaataacctt ggaatcaaca ttgttaaaca agttttctac aaatttgtgc
    46861 caatggctgg tttcatctga cagagcatca gtgattacag taaataactt taaataagtt
    46921 cccttacggt caagtttttt agccaactcc attattttag gcaacttatt cagaggatcg
    46981 ttggctatgt aattgattaa ttggtttagt gcaattgtct ttacattctt catagcaaat
    47041 cacccaaagt catacctttt tttttactaa ttaaaattct tcttctaaaa tgtcaagtat
    47101 ttttttataa gagtattgta aaggttcaca ttttcagtca aaaacaggaa ttttatcttt
    47161 tattccagag tttaattctt cctttattct ccacaatgtc cggttgaaat catttatttg
    47221 aatgagaaaa atggttgatc ttaattagtt tgtcattccg cacttgatgt agaatctgtt
    47281 ttaatgtatt taatagtatg gatttctgct ttcacaagaa tgataagtat cagggacaag
    47341 tatggtgatt cctaccttct caggaatgac aattatcggg attccttcct tctcaggaat
    47401 gacaagtatc gggattcctg ctttcgtagg aatgataagt atcgggattc ctactttctc
    47461 aggaatgata atagaatatt ttaccaaaaa gatatgagga attccgtagg aatgacagat
    47521 attaacaacg ggttttaacc cggtgaaaaa gagtccagaa tactcttttc ttcatcttcc
    47581 aaaaaaaagt tccgtaggaa cgacagatac taacaacggg ttttaacccg gtgataaaga
    47641 accaaggata ttcatttctt catcttccga gaaaaagttc cgtaggaacg acagataata
    47701 acaacgggtt ttaacccggt gcaatatcaa tataaccttt tttcttattt ttctgaattt
    47761 ctgctttcgt aggaatgata aaggactttt cattacagaa atgatattga aaatctctgt
    47821 gccaggttca aaacccgttg ttatttttct accagtccta cggaacatat tccattattt
    47881 atggtttatg gataaaaaaa taaaataatc tacggcaaat agaaatttgt caccctattt
    47941 tttacatttg ccataattag gttactaata tataaattaa agcattagct gaacaatata
    48001 tctatctctt cctgcgatat acagatagct tattattttg ttaataaaaa agacctcttc
    48061 cctaatagca ttgcttactc attgcttacc cattacggat gggattagca atgagtacgt
    48121 aatgctaagg caatgctcta aggaaagatt gcttccctat aaaatatttg tccctgaaac
    48181 tggaaaataa gctaatgaaa agaattaata taacttatca ctgggttaaa acccgttgtt
    48241 agtatctgtc gttcctctgg aacttattct aaaattaaga gataagatgt tgcctttttc
    48301 acttttcacc gggttgaaac ctgttgttag tatctgtcct tcctctggaa ctttttctcg
    48361 gaaaatgaag aaatgaatat cctgggttct ttttcaccgg gttaaaaccc gttgttagt
//
LOCUS       DAOOZV010000008        47851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_867_length_47851_cov_58.239434, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000008 DAOOZV010000000
VERSION     DAOOZV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 47851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47851
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_47851_cov_58.239434"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(680..1252)
                     /locus_tag="PLW08_01765"
     CDS             complement(680..1252)
                     /locus_tag="PLW08_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51361.1"
                     /translation="MKYAFLLLLLAEIMMLSALTFEAEELNFYLENNLWEIDGLFHFA
                     NYDSLSTHKLIFFPVPSDSLCLPPKIKAIAIQDSNGAEVTMLNQAKNGFTFLLSLPAL
                     SFCTLHLDYTQTLKSNYAKYIITTTNSWGRPLPYAKYTLHPGINVQIRSLPFSVQKQE
                     GRNYIWEFFDFAPEKEFEVTFQTIPAEDQN"
     gene            complement(1249..1647)
                     /locus_tag="PLW08_01770"
     CDS             complement(1249..1647)
                     /locus_tag="PLW08_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51362.1"
                     /translation="MKTQIWVNNPITNEIAFNTQFFAEPGALIPFSVQLPVSVEENIV
                     SIPAGKLHLYPSVLNPHWNESIRISYDTKLTGKAELVLYDLKGRHLAEKVYDQNEIEW
                     QLPKLSSGIYFVRLTNQGKALGTAKLIILK"
     gene            2096..2935
                     /locus_tag="PLW08_01775"
     CDS             2096..2935
                     /locus_tag="PLW08_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51363.1"
                     /translation="MHLRQAKVIKSILNALFGDYNGIQVFVAPITLLYWIDSGSLLSS
                     ATSLLSFRMHYLPLLAFLIIFVFSVFMLIKIKLLYNCNNSEYLDMVIQFNVSVMALVL
                     IGLIIYAISSFLAYFYGIKGTVKSGLLLLFKLYTVFLILYHYLFNVVLTPYYQKQYGH
                     PRALKAFLSWARNNKFLLFRYILLILLVVFFAVRFYQLILRFALMPLIGFIDKYTGIS
                     IKFKLYPFVMIEDIFVNVLVLTGAFLVSNLFFFPLIWVLKYLVNRFIPFKNLLRTSYA
                     QSA"
     gene            2919..4208
                     /locus_tag="PLW08_01780"
     CDS             2919..4208
                     /locus_tag="PLW08_01780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPL51364.1"
                     /translation="MPKVRKKKLRKVKRRRKGGNPGIFILTGFICCFLIWIIIKEPAD
                     KNSELSKAETVSKNSELPVKPKKKTKKEVKAISPSSTQILTKDKKAQETTIDNTKNTP
                     ESVITTTIEKLGIPKSYFRKRNTENLTIFNVPIDKSEMDLVYANMIFKGELERNGAKF
                     IKGTDSAGKQSLLFSFPDTAKQYAINIFYDNNLYRNKQKKRTITIVVDDFGTIGGDLL
                     TGFLALDKEVCFSIFPEEEYSVQTMKLAKEQGRLTLIHIPMEPIGYPEVNPGKNPILV
                     QYDERQIEKIMTRFIEQLPYCEGANNHMGSLATTDEEVMNSVMAILKKHNKFFLDSRT
                     TNVSVAYSVAQKNHLKSYRNDLFLDSPNISQSTMDAKLNQIIELSNRNQNVIAITHCH
                     NYDKLDYLKRFINRLKAAGFTLIPLSDIDKYNVPEIL"
     gene            4219..5034
                     /locus_tag="PLW08_01785"
     CDS             4219..5034
                     /locus_tag="PLW08_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPL51365.1"
                     /translation="MSLIQSILMSILQGLTEFIPVSSSGHLVLAQHFFGIGDSENIVF
                     ELFMHLGTLLAVLVFFRKTLWELIKSLFSWGNTVNREQHRKNRTLVYYLIISTLATGV
                     IYILFGDFMEAVFNMPMLVAILLLVTGFIVFVSDYFKDKGIPACNIGFLQAVLIGIGQ
                     GFAILPGISRSGTTIACSLVTGMKRKDAAQYSFLLSIPAILTGNLSQYKAFANLKPQL
                     LINYLAGFVCSFLVGYLVIAFLIRLIEVSRLKYFAVYCWLIGLLSIVLIILGF"
     gene            5034..6035
                     /gene="holA"
                     /locus_tag="PLW08_01790"
     CDS             5034..6035
                     /gene="holA"
                     /locus_tag="PLW08_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPL51366.1"
                     /translation="METSIFALDFTADKVRLGSSYLLIGTDSYLVDRVLDTIKAKLKK
                     KEKVDTFIIYGDEVKSPELADLLDTYSIFSAAKLIIIKNAESLKIKELSTLADYFSSP
                     SKIQSLAIVTEKADARHENWKKIKAGTKFITCDPPPYGGAIRAWLDKALKDINKKMTD
                     KAIEEFLNRIELDYYNAANELTKLDLLTRDRTTITEEDIMQSLGTTRSGTLIDFYRAL
                     GRKQAKQAIEAMEKMLFADWKPLQILFQLTRFYFNIWKILLLKKAHITDNEIVSKYIT
                     DFHPNQRREFLDLSRYYNINALEKIFALLLETDQQFKLTVAEPQILLCNCLLKILDA"
     gene            6035..7498
                     /gene="malQ"
                     /locus_tag="PLW08_01795"
     CDS             6035..7498
                     /gene="malQ"
                     /locus_tag="PLW08_01795"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPL51367.1"
                     /translation="MRKTGILLHISSLPSDFGIGDFGPEAVRFAEYLKAEGYTYWQIL
                     PINHCGYGNSPYNPLSAFALNPYFISPELLYQQGYINQLMLEAVKIPRSNVTRYEQVY
                     ILKDKMLAQATANWMLANRTDDYIEENAPFLKPYLTFLSLSKFYDDTCWYNWNEEHRH
                     YSEKLYQKVRLMNSSYFNLRAATQAMLVEQLSWFKERLQELGIILIGDLPLYLAYDSA
                     EVWAHPEYFDLDEQGRRLHFAGVPPDAFAEKGQLWGNPLYRWDVLKQDGFQLFMERIK
                     QALGYLDILRLDHFIGYVNFWQVEPELDANGQPVLPKDAIKGSWVRALPEDFFSALLQ
                     RFSIKRFIAEDLGILNDDVCRIRERYGFPGMIVLQFCFEEGVPDVKKYPPERWLYTGT
                     HDNQTVRGWFESLSPNSSSYKHLQEYCALHQFTDCQAHLNAENIHLIMRNIALSSSCQ
                     QVIFPYQDILGLGDEARMNIPGTALGNWQWRLENLNF"
     gene            7525..9132
                     /locus_tag="PLW08_01800"
     CDS             7525..9132
                     /locus_tag="PLW08_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPL51368.1"
                     /translation="MPKLMLLGDEALAQGALDAGISGFYGYPGTPSTEIIEYAQRSKQ
                     AEENNVHRTWSSNEKTALETALGMSASGKRAMVTMKHVGLNVAADPLMNSAITGASGG
                     LIIAVADDPSMHSSQNEQDSRYYGHFAMIPVLEPSNQQECYDLAFYGFELSEKYHIPI
                     LLRLTTRLAHSRSGVSRRNPLPQKELKKPEDIFQFMLLPAIARKKYQHHLSLQKDFLA
                     ESENSPYNVYTAERKNTKLGIITTGLAYNYLREAYHGEEIPYPVLKICQYPLPEKQIR
                     QLYETCDELLVIEEGMPFVEEQLKGLAFPGLKPIHGRLDGYLPRAGELNPNLVAKALS
                     LPDTIGRPVPDIVVGRPPALCVGCPHSDTFLSLNEVMAEYGRGNVFSDIGCYTLGFMP
                     PYNSINSCVDMGASITMAKGASDSGLFPAVAVIGDSTFCHSGITGLLDCVYDKANVMI
                     IILDNATTAMTGGQNYTGYGKLEDICLGLGVEKEHIRVFVPLKKNYPEMIQIYKEELA
                     YNGVSVVIARRECIQTLKKKNKMEIQE"
     gene            9129..9695
                     /locus_tag="PLW08_01805"
     CDS             9129..9695
                     /locus_tag="PLW08_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPL51369.1"
                     /translation="MKKDIILAGVGGQGILTIATILGTTAIKRGMNLKQAEVHGMSQR
                     GGDVQSHLRLSDETIWSDLIPFGSADMILSVEPLEALRYLPYLKDDGWIITNSTPFKN
                     IPNYPPEEEIYKEIKKIPNYVLLDADTKAKEQNASRSMNIVVLGAAIKHLGFSKEEIE
                     EAIKTIFAPKGEAIVEANLRALQAGMEG"
     gene            10057..10656
                     /locus_tag="PLW08_01810"
     CDS             10057..10656
                     /locus_tag="PLW08_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL51370.1"
                     /translation="MSAAQNKKKLILDIATQLFTRFGFAKTSLDEIAAAAQIAKGTVY
                     YYFPSKEDLFIASVEAKTREYFKMLRQHINSTTGFEEKLTEFLSFPIKLIYENMPILI
                     ECLINIPQSYQEQLIQFRVKSREVMMQILSEIIQLGKQEDLIDERFPEDRLCEIIMDW
                     FMIGEPSLKIVDVPRLMTMIERDSDFIIKMLLYGIVKRG"
     gene            10660..12039
                     /locus_tag="PLW08_01815"
     CDS             10660..12039
                     /locus_tag="PLW08_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPL51371.1"
                     /translation="MRKTLIVLTLLTLLASCVFAIDLETSITKAKQNNKELLMAMEEI
                     KKADESYKQVRGTAYPQLNLQGAYGLSKTYYPDSAIPAKMNISAGLDDTATDNEEYLA
                     GVLNKIVNSMIPESPSETGSFALQLKMEQIVYSGGKLGSGIKAGNYYRQIQRLNYKVK
                     EQDVVLKTTELFYQCLLAKKLWDVQEEGLDIAKRHLRRVELFNQEGQVSEFDLLQAKL
                     GVAKLEPQVLKAKNDYDLAVSAFRKQIGEEESDIVPEGEFVLPEKMELSLEEATEQGL
                     KQRNEVELIKLGTQLKELQYKVEKVNFLPNVALSADYSLYTAADEYAIEKDDFGHKYG
                     FSLGFQIPLFTGLTNTAKRNYARYDYQKAKLQQRDTEELIALQIKQDYQKYYHAWENH
                     NVQTENIRLAERGLQLAQVRYENQVGIQLEVFDAQLTLQTIKLQYYQSIYEIISADRN
                     FKKSIGITL"
     gene            12055..13080
                     /locus_tag="PLW08_01820"
     CDS             12055..13080
                     /locus_tag="PLW08_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL51372.1"
                     /translation="MKRYLIILTALILILISACGKKQEEGKSMEQIYSEEGVPVRQII
                     IEPTTFRQNLLYNATLNGMEETTVQAMVSEVITKINAKVGDRVAKDQIIVSFPQNTPS
                     AQYEQALTAFNSIKTTYERMQRLFAQGAISQQDLDNVETQYKVSKANLETSDKMINVR
                     APISGIITAIYVNPSEKVYPGKDLFTIASTNGYKATIMVPDTEINKIKKGTKATATWL
                     ETTISGRVTEIPLAMDNATKAFKVEVSFPGINKKINYGVTAEISIEVFSKPNVIVVER
                     HQIVSENGTKYVWLNQDGKAVKREITTGLDNTLAFEITSGLNPGDLLITEGINMLTEG
                     AKLRVIE"
     gene            13094..16177
                     /locus_tag="PLW08_01825"
     CDS             13094..16177
                     /locus_tag="PLW08_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPL51373.1"
                     /translation="MFLTDLSINRPVLVTMAIMVFIVFGVLAYFSLPINLMPDLKLPY
                     VVVQTVYSGAGPREIESQVTEPIEEAVATVSQIDFMQSYSMENVSIVLVAFKLGKNID
                     VANQEVKDKVDAVIRNLPDGTDRPIVLKMDITAFPIMDLVLSGNMPPKELYELADGTL
                     KDRLSQIPGVASVSLIGGAKRQIDVRFTDKVVFENKISLAQLSAILAAANLDMPGGNF
                     TQGTQEYSVRLKGEYQDVEDIRNTDIPTVFGVKKLSQLARVEDATEEVRSKAIYFNVP
                     ENVKDENIVRLSITNAADGNVVTIAEEVTKEIPILNKELPEGVKLEIMRDDSIFTKDT
                     INSTLENILLGILFTGLILFIFLHDFRSTIIVAISMPYCIISTFVFMQIFGYTFNIMT
                     MMGLSTAIGILVSNSVVVLENIFRHKDMGNTRKVAAQIGTNEIGTAVLASTLTNIVVF
                     LPIATMTSMVGRFFKEFAVTVTMATIFSLLTAFTITPMLASKIIPKEKRTIKWGRKFD
                     NVFDKFGNYYAKFLGYTLRSKKTSVSILLFTLLALLITFGLAFFVGMEMMPPVDQRNL
                     SISVEMPQGTNLEQTAKTMDIIQNRLSQHKEVVHILTNLGSGSFIDTGTNLATSDIKL
                     VDKNERKYTANQLADILTKELADIPNAKIKVTGSMLGMGGGGRSGLQFMIEGQDNARL
                     EQLKNEVTKAIEDIPGLINLDTSSRYGRSELTLYPKRDKLAAAGATVYDLALALRANV
                     EGLVSTKYREAGNEYDIKISLEDEVVDTPDKIKNLSVVVMGKSYLVSQLADIQFSSGI
                     NRITHYDRYKTVMFTGDVASGYTMSDITSQIEERLDKIKFPTGYRYQWGGQAKMMSET
                     TVDMIRTFILAVILTYMLLAAILESFAQPILILATVPLALIGVILSLLITGNTFNIVS
                     MMAIIMLVGIVVNNAILIMDYVNIKRREGYSVHDALMEAGKMKLKPIVMSTLAIIAGM
                     LPMAIGIGSTGSEMTRPMGIVSIGGLIVSTFLTLIIIPAFYFLTTKNIHAKMESQ"
     gene            16200..16715
                     /locus_tag="PLW08_01830"
     CDS             16200..16715
                     /locus_tag="PLW08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HPL51374.1"
                     /translation="MKKITRLVSIALLAVFIVVSVFGCYGNMSLTKKVYRFNGSVGNK
                     FVQSIVNWAFWICPVYEAAMFLDIVIFNTIEFWTGSNPLAMNEGENIIKYAEGPNGNY
                     KFEISQNKIVISQPENKDNNQNIELNYDPVSESWFMNSNGTTQKIGYLDGNQLKLLSQ
                     SGEEISLDLSK"
     gene            16725..16952
                     /locus_tag="PLW08_01835"
     CDS             16725..16952
                     /locus_tag="PLW08_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPL51375.1"
                     /translation="MIAKEKIESILAKVRPSIQADGGDVELINIREDNVIEVRLKGTC
                     NGCPMATLTLKAGIERLIKEEIPEVKEVIAV"
     gene            17276..19735
                     /gene="sppA"
                     /locus_tag="PLW08_01840"
     CDS             17276..19735
                     /gene="sppA"
                     /locus_tag="PLW08_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPL51376.1"
                     /translation="MKSIVKALILLLPFLFCSLAMAQTKSDSIFVFLNKQDFPIASVD
                     NMFIPISNPSLLGTGSASGIGLAYLNDEKEWQNHYWIFLNTDLLSYIYERDHSYKYHT
                     LALGAELFPAHILPNLYAGTNYRWQEGGFEDGSFRTGVTYRPHNSTSLAFTWDNPKHQ
                     STYYRLGLAVRPFVFTDAIADYRLELSVDANYARYEMDKDYEFKKPVLGIQTQILDGV
                     KVGATYNWEEESALVNFSICPRNLEAGGLLRSKKNDNYGIAWAQLTDWEHKPFLGHTK
                     PSWYKMDLKGNIVTYTAPKYAIGKIKIYDTGDKSIESVIDNIKQAKDDPEIEGILLKN
                     PSFSTSLALQEELIDAFNDFKSSGKKVCFYYNNIGNGGYIFASSIADKIYLNPTGSVD
                     LRGLSISSPYLKNMLASLGIEVLNFRSHEYKDAGNMFSEERMTPAEREAYESLLQSLY
                     DQILQSMETGRGDRLVASADEIINGGPYFIANDALEKGLVDALIYEDQLNKQLKKDFK
                     FSSQHKELTEYREYAWAKPKENLVAVIYASGNIVSGKGTPGQKIAQETTVDLIRKARK
                     DKQYKGIILRVDSGGGSAQASDIILRELELAKTENKKLIVVSMGGTAASGGYYISCNA
                     DKIIAEPSTLTGSIGVVGLMFNGTEMFQKIKVNWDTVKKGEHSDMGSIYRPWTEEEKQ
                     MVTRSIENCYDDFVKKVDEGRPNMTLEQVKQYAQGRVWTGEQAQKIGLVDELGGLEKA
                     KESMSELIGKRGKLTLIDATTKKEGVKISINIGELNVFTPVKAMNAVNSDYIKLYELW
                     SDFGQDKALMLCPVIPETLQF"
     gene            complement(19905..20684)
                     /locus_tag="PLW08_01845"
     CDS             complement(19905..20684)
                     /locus_tag="PLW08_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPL51377.1"
                     /translation="MEYDPLKNRLARAIDMFPALRKLFYMAFNLIFLRQRYVLKEIRN
                     LFRETDKINLYDAGAGFCQYSAYVLSHWLNSNAFATDLKTDYLRSFSAYADIYYPGRF
                     TYKTADLQTFTPSQKFNLALAIDIMEHIEDDVSALNNLYNALKKKGYLLLSTPSDLNE
                     TAKFTSEHIRAGYNKKELENKLNRIGFRIIKSIYTYGTYGALSWKLLIKIPLQLISKK
                     LYLLLPLYYLLVYPLAEILMRLDMKTDNKQGTGILIVAQKP"
     gene            20761..21624
                     /locus_tag="PLW08_01850"
     CDS             20761..21624
                     /locus_tag="PLW08_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPL51378.1"
                     /translation="MIVKEINDIQRVNLHLHTNVSDGNISPKELIKRSQEIGLDLISI
                     TDHDTADAYTQIPDNILPLRILPGMEISSLYKGDDVHILAYGCDFNNPALRAMTEMYL
                     KGRKERAQKIIKLLTDMGMPISLEEVIAIAGSRELIVRPHIAQVLVNHKFCQTRNEAF
                     EKYIGDNKPAYVPKPEVSVSDVISIIHQADGFAVIAHPGKLAKLSYLEDIITMDIDGL
                     EVWHPDHYQWEIENFIKIATEKGLYMTGGSDYHSEQDKHNLFDIIPLPEVVLTSVKKL
                     WKEYQCRVKLR"
     gene            21603..22139
                     /locus_tag="PLW08_01855"
     CDS             21603..22139
                     /locus_tag="PLW08_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51379.1"
                     /translation="MPREIKVKSIRNELPATYRYSPWTRFIMLFVALAICIYAIFFFV
                     KFVTGTTPMFFKVLPLIILFVALDSVFKQLTSLYSVTFYEDRICFGFLCKPKIIIPYE
                     NITSIDLTKQISFSIILKYKDKDREKIFKTSASFPKILEILLNLADFAPQAQLNDLLG
                     KVVSNLRNLAGKSETEQV"
     gene            22120..23292
                     /locus_tag="PLW08_01860"
     CDS             22120..23292
                     /locus_tag="PLW08_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPL51380.1"
                     /translation="MKQSRFDEIIDRRNSGCYKYDGLKMLYGKSELIPLWVADMDFAV
                     SDEIISALSERLKHPVFGYNLRLDDFYEAIISWQERRFDWKVQKNWIIAVPGLVPAIS
                     LAVLSLTEPGEGILIQTPVYRPFYDAVLDHKRKLLTSALVNTNGIYSIDWQDFEEKLK
                     QAKLFIFCSPHNPVSRVWTKEELTKIGSLCAKYKVPVFSDEIHADIVYPGYKHLPLAS
                     LNDFSEICLTGVSPSKSFNIAGLATAVVIVSNSEIKNKFNELNTKLHLYLGNTFGIRA
                     LIAAYSESENWLDELLCYLDKNRIYITEFVQNELHGVKISPIEGTFLAWLDFRNWGLT
                     EANLQEIIVNKAGLALEPGTEFGKDGEGFMRLNFGCPKSILEIALKRLQILAREYG"
     gene            23289..24104
                     /locus_tag="PLW08_01865"
     CDS             23289..24104
                     /locus_tag="PLW08_01865"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPL51381.1"
                     /translation="MKQEIIELYNNPPEKWTAKHKALFAAFIDALNKGEIRSCEKEKS
                     IWKVNEWVKMGILLGFRMGELTIYQWSEAKPFFDKDTLPEKQFTLADRIRIVPGGSSA
                     RNGCYISAGVTIMPPAYINIGAYVDRGTLIDSHSLVGSCAQIGKNVHLSAGAIIGGVL
                     EPVGMRPVILEDDVFVGGNTGIYEGIIVQNKAVIASGTVITASTPIYDSVREKFLERD
                     SGNSFTIPSKAVVVPGSRRLKSNPDFQIACPIIIKYRDDKTDKAVELEQALRI"
     gene            24360..25751
                     /locus_tag="PLW08_01870"
     CDS             24360..25751
                     /locus_tag="PLW08_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HPL51382.1"
                     /translation="MNSLGFKFPVQERKTALYNLEEEWYLPLLSKHLGIPMQSIKRSN
                     FGQYSMAVNYLTSVLEEAAAINKVAVYNIDHMAQLLFYGPDVVTLLNRALTGNFTDMK
                     VGQCKYTLLLNEQGGVQDDMILMRVTDTSFILVINAGHDITDTIEVDGEKQEYLADID
                     RIMQCKKEGEEVWAKDISDTLLKIDVQGPLSYKLLKEVYGESVLKNRNNPEKNMNFFT
                     FNEFERNGHHYYLSRTGYTNRWGWELYIPVAVAEEDAKIIISKALDFGGLLVGLGGRD
                     ENRISAGPFGLPLMGQEYDPYHTPVNAPLFETAVDMNKEYFVGKEALAKEIAEGVQKR
                     LYIFVSEGIVSNRGIYKDGKRLGTVTSSINSPNVSWEMRLAIGSKRKNVNEEKGTAAI
                     GMGWFYSPLFEKDDEGKEIGEIDGKPIRIPVEFYREDENRKPTGTPVLGYVTLEGITP
                     ATAHKPLKNIENL"
     gene            complement(25911..28265)
                     /locus_tag="PLW08_01875"
     CDS             complement(25911..28265)
                     /locus_tag="PLW08_01875"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPL51383.1"
                     /translation="MHNNIPFLWIIAPLGAILALIFAAIFFRQVKKQDPGNAKMQEIA
                     GYVREGAFAYLKQQYKGVGIFFIIAFSVFQIMAWGLGVLHWLIPWAFLTGGFFSGLAG
                     YIGMNMATLASNRTTQACTKGLNKGLRIAFRAGSVMGLTVVGLALIDISAWFFILNLL
                     KFPLQQITVIMLSFGMGASTQALFARLGGGIYTKAADVGADLVGKVEANIPEDDPRNP
                     ATIADNVGDNVGDVAGMGADLYESYSGSILATAALGMSAVTMLGPAYADWAMKFVVAP
                     MILAGLGILLSIIGIFTVSTKENAGTKELMFALNKSVYSSSILIAICAYFVTKALLPP
                     DYYFGVFISTVIGLAAGILIGLFTEFFTSHSYKPTREIANQADYGPATVILEGISVGM
                     RSTAAPVLIIVIGILSAFICSHGFSSIEMGLYGIGFGAVGMLATLGVTLAMDAFGPIA
                     DNAGGNAQMCNLPEDVRMMTDNLDSVGNTTAATGKGFAIGSAALTAMALLAAYLEEVR
                     GALHLVGTKAGKAVFLHTNYNTKFTENIPLAKASIADFIQFFQINVLNPKFLLGVFIG
                     AMVAFVFSSLTIKAVGRAAGKMVKEVRRQFKEIPGIMEGTGKPDYAKCVKISTQAAQR
                     EMLIPALVGLLSPIITGLIFGVPGVLGILAGALSTGFVLAVMMNNAGGAWDNAKKYIE
                     TEKKAKGTEIHKASIVGDTVGDPFKDTAGPCINILVKLMSMISIVFSGLIVSYCLHVE
                     KINPPFTKVHLDRKVENKVIMVDKDSLDVNGNYPPTTTTEIIVK"
     gene            complement(28372..29286)
                     /locus_tag="PLW08_01880"
     CDS             complement(28372..29286)
                     /locus_tag="PLW08_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL51384.1"
                     /translation="MKIATTPRVLYYHIVADKFPEIYHSGISVSNFFRQLGQLKRRGY
                     KFRSLSEVLANPKKFNAKDIVLTFDDGFSTNYPVLMYLCKEYNIKPTVFLIGKCVDNK
                     EMAWNHKLLLMKRYCSPVKLSAQIDNYVPGATISSFFSRIEMSEKDTVTDSLWKEVMP
                     FSESDYLQKHKPFLSLAQMKSLQKAGVEFGIHSWSHPDFSRLIYPEAYTELDLCFKQM
                     EELSVPCKRYFAYPYGRPAQNEIETRLIKNFQLSATFGISYGLGDNSLPYTRWQRIKM
                     DGRKLTNREQFLLLPLLRSIKASRFNFL"
     gene            29808..29885
                     /locus_tag="PLW08_01885"
     tRNA            29808..29885
                     /locus_tag="PLW08_01885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29843..29845,aa:Pro,seq:cgg)
     gene            29934..31691
                     /locus_tag="PLW08_01890"
     CDS             29934..31691
                     /locus_tag="PLW08_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51385.1"
                     /translation="MAGDKQTFTYYLKLLFPFVKKDLGLLIFGLFAMLVTSGLRLLNP
                     LILAQIIDHSIPDKNMAQMFQYGAYFVIVVLLSGLLSYLQILLLSRLGIKIITKFKGN
                     VFSHLLKLPISWFNKQPVGELIARVESDSERVKVLFSDLSITIIGNVLFFIGGFVILF
                     ILQWQITIYILPAIILCGIGYWFLFKYLNKFFRLIRERYAIITAKITDYVQGIQMIQA
                     LNQETKALQDVEKASLDKKKVEVHTQIIEYGSQSIFSFMINVVFIIIVILISAPKIIA
                     GTLTVGILIVFIQYIYQLVWPLMQISENVMQIQRSFASLKRILELTELPTEDDTYTGT
                     KIPVFEQEIKFENVWFAYKEGEWVLKDISFTIPKGKKIALVGPSGSGKTTTVSLLCGF
                     YPVEKGKILIDGVPLSEMDFREWRKKIGLILQDVYLFPGSILENVRVYNDQISEDKVQ
                     KAISIVQLDDFIKKLPEGINAELAERGQNVSQGEKQLISFARALAFEAEIIIMDEATA
                     SIDPQTEAKIQRSMENLLSGKTALVVAHRLTSVLDADEILYFSDGRITARGKHNELLQ
                     TSPEYQKLVELQMLRVQDE"
     gene            31684..33450
                     /locus_tag="PLW08_01895"
     CDS             31684..33450
                     /locus_tag="PLW08_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51386.1"
                     /translation="MNKHIKWILEQYKAQKWFVLIMVVLTLLSSLVSIAFPYVFKEML
                     DLLKDILQFPQKYPEPMKAVYRIIKIMFAIGLAQLVTGLYPGIRGWVNMRFEHSLRVL
                     YFKHILTKDFSFFQKFRTGDIVTRLTDDLSDYPRVSWFLCSGIFRAFNSFSMIVFALV
                     VMFSINVKLTLLSIAPLPFMMLIFYFTADKVYTNYNRNQQAISAINNQLEMSFSGIRI
                     VKSFVSEEKYNRFFDLALAKRFKTEMSMVKLNAILSLIWQYIDYIAQIGVILFGGYLA
                     VKGEITVGTFFLFYTYLSMMIYPLIDLPQLFVSGKQAFVNIDRLEEMKNYPTLNDEFK
                     GHHKVKEFSTLSFENVSFQYPDKPSPVLKNVSFELGSGEKMLILGATGSGKTTIANLI
                     LGLLKANSGIIKINGKPINEIDIKSLRNIVAYVPQEPLLFGGSVKDNILFAVEQASEK
                     DYQIAVKTSQLEDEIASFPDRDNTFVGNRGLSVSGGQKQRLTIARALIKKPQLLILDD
                     ITASLDAENEEKLWQALRENYPDISAIVISHRLSTLHYVDSVLFIDSQGYAHKGSHDY
                     LVFNNQEYHDFLREHLKQENEK"
     gene            33511..34608
                     /locus_tag="PLW08_01900"
     CDS             33511..34608
                     /locus_tag="PLW08_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPL51387.1"
                     /translation="MPRKCLIILLCAFFTLSYADELEDKMRQLREMQRQLESTEQKVK
                     QTQTKKQQTESEIKRTASLKQRTEKNLQKLKTEEKIVQDSLKSVQQRIAVTEMRLQDM
                     HREQNAELDLLLRVDKSYKKLNINHRDQRLLKDFIQGTRQKIDILSGYKIALTQVQEL
                     HKAHATKVSRGMREESSKKSSYEKQIKTLSSQSQKLTEEQKRLQAQVEKLKKDSAELE
                     GLIAQLMKQQGKEPPSYQFTGKKISWPLRGKIIRSFGQETRSYGTSVVSNGIDIAAKE
                     GTKVVAADDGEVIFADRYGGQGKLIIIDHKNGFFTLYAYNSELLVSRGTKVKRGQTIA
                     KSGMTGSASEPSLHFELRKDGKAINPIPYLE"
     gene            34622..35722
                     /locus_tag="PLW08_01905"
     CDS             34622..35722
                     /locus_tag="PLW08_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPL51388.1"
                     /translation="MLVLKDPGLRKLEKFIANNPLTHSTILHWTGSMWGIGCRLSSSE
                     TIARIETLKQRADKQGFIALIPDMSVLEQDKIPAPIRPLMNQYWPGNLTLVFNYDDPQ
                     FAGIKNGNKVALRVPSDPALRALISLLGEPLISTSVNVSGLMPEENYERIVRNYDSWF
                     DFALLPSSREMKRPSEPSTIIEYIGSEECGSNDDIKCIREGSIPFYEIKQAFQLPVVM
                     FVCSANICRSPMAEKLFRKMIREHNLGLATDSCGLLPGGRMISLESLQLLLERGIIDA
                     EQHISKQVTPQLISSSWLILTMEERQRDLLRSAYPNNARRIWTLNEIVDEQGDIDDPF
                     GNNLESYKKTCEIIEDRLQRLTEKLKNNELRI"
     gene            35734..>37385
                     /gene="purL"
                     /locus_tag="PLW08_01910"
     CDS             35734..>37385
                     /gene="purL"
                     /locus_tag="PLW08_01910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPL51389.1"
                     /translation="MKKDEITPELVAEHNLSPEEYERILDILKREPTFVELGIFSVMW
                     SEHASYKNSALLLKTLPKEGSFILAKAGEENAGLIDIGQGLAISFKIESHNHPSALEP
                     YHGAATGVGGILRDIFTMGAKPIAVLDSLRFGKPENPKVQHLLKEVVRGIADYGNCFG
                     VPTVGGEVYFEDSYEGNPLVNAMAVGIVKHNKIARSTASGIGNAVLIVGAKTGRDGIH
                     GATFASVDLSEKSEEKRTAVQVGDPFLEKLLLEATLEAIEEGLIVAIQDMGAAGLTCS
                     SSEMAGKGNVGMEIDVSLVPQRETGMTPYEIMLSESQERMLVIVKPENLERIKSIFAK
                     WDLDAVQIGKVTADKILRVFYGEEKVAEIPAETLILGGSAPVYKRASKYPEYLETLYN
                     FDPDKVPEPSNYAEVLFKLISSPNLCSRFPVYNQYDYMVQIGTVVEPGSDAAVIRILD
                     TDMAIALSTDCNGRYCFLDPYSGTVSAVAESARNVACSGAKPIAITNCLNFGNPYKPE
                     VYYCFSETIRGMSDACKILGTPVTGGNVSFYNENPEGAIYPTP"
     assembly_gap    37386..37485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37486..37820
                     /locus_tag="PLW08_01915"
     CDS             <37486..37820
                     /locus_tag="PLW08_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPL51390.1"
                     /translation="LINSAHDVSDGGLLVAVLECCFAPDNIFGATLNFAFSDKPSKVY
                     FGEAGGRILISALPENCQAIKETAEKHNIEYHELGYVSGKAKFVVNNHIEEDIEILYS
                     GWKQGIKI"
     gene            37839..38375
                     /locus_tag="PLW08_01920"
     CDS             37839..38375
                     /locus_tag="PLW08_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51391.1"
                     /translation="MKMDFIFGNIFWGLLIILLGLSIFLKGFNLHIPLVRVFIAIIII
                     MFGIKLLFGIKTHKYNKPKHYSRESTINYSGSRNEYNIIFSSGIIDLTDLPADAKDVE
                     INVVFGSGTVILPDSIVFNINPTSVFGSTVLPPKRPELQGKPIDIESNVVFGRLEYKY
                     APPPKATAKHAETEKETF"
     gene            38492..40654
                     /locus_tag="PLW08_01925"
     CDS             38492..40654
                     /locus_tag="PLW08_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL51392.1"
                     /translation="MNTVKKSLILLLFIFFLNSLTAEKLGYALSGGGARGFAHIGVLK
                     VLEEEGIHPDYIAGTSIGAIIGALYAMGYSASELEEMCLKLNWNELTRDTHQRKDLYI
                     GQKRWAPYGNAVFELNESWVPQLPSSVFVGNNLNLELFKLTAPASQIQNFNELPIPFI
                     CNATDLLTGEAVSFTEGSLLQALRASISIPSLFEPFELNGHYYIDGGVSQNMPIGLLH
                     QMGADKVLGIKVNSTLRDEERINNLVEILDQTINIGITRNLKEHLDECDLLIEPELTQ
                     FVSTDYQHLQDIIAAGEESARQMIDQIRAFKNEFYSSSPKITTFDKKLNSFYITQINV
                     QGNKNISPSKVREYVGLETGKTYSSEEIANACTNAWNSQLFKTIYPVLQKNQDNQYDL
                     YIYVKERERKQLAVNLVYNSEEKLMAGLVLSLNNYLLKNSRLLAELKLGGRNELNVDY
                     VKNFGEEWGAYYRLFPYVCERTIYEYSEHHKINSFKSTEFGINSGVGVFAKDLAIAEF
                     FVYTDQTNLHSDISQTPLLPESSVVSGFGIKGYHESLDDFIFPTSGARVFGKFNFARY
                     SEISDFIYSCFQGRAEFYVPVRNIFSVHSSIYYGSYLNSTEHDILDPFIISGTDGFMG
                     YSRYEVSAPHYQILSFGFSSLPKNRYFIDAGFQGMRYSEKDVWSNSEDWEYCGYLGLG
                     YKNSILPTKLYVSLNKDKKVHSFLSIGYDFDIFFFSRR"
     gene            complement(41125..42528)
                     /gene="glmM"
                     /locus_tag="PLW08_01930"
     CDS             complement(41125..42528)
                     /gene="glmM"
                     /locus_tag="PLW08_01930"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPL51393.1"
                     /translation="MNKLMTGISGVRGVFADTLNPIIAIKYAAHFAILQKQSYPERKP
                     EIIVGRDSRTTGKAMLHSIISALISVGCDVTDLGIVSTPTLLLKVQNSDAIGGIAITA
                     SHNPPEWNAMKFVDADGMFLSPEKADKFLSSVEEDIPWAKWKAMGELKIDNTAIQEHI
                     AKILNIPYLNIEQIRSRQFKVVLDSVNGAGGLISPLLLKELGCTVYEINSEPNGIFAH
                     IAEPLKENLGQLEETVSFFKADIGFATDPDVDRLAIVSEEGKCIGEELSVALAELFVL
                     PKKKGDIVVNLSSSMLSDDIAKSFGVQVQRTKVGEINVGKKMQEINSPIGGEGNGGII
                     CPDVHYTRDAIAGMALILGLLAETNKPLSAIVNSLPKYYFAKDKIMVDASLMETLMND
                     APFFFQNYQLDTRDGIRAIGEKHFIHIRKSGTEPIIRIYVESDSPEKSRELCEIVKTM
                     LGEINTFKDTDNNERNM"
     gene            complement(42529..44316)
                     /locus_tag="PLW08_01935"
     CDS             complement(42529..44316)
                     /locus_tag="PLW08_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL51394.1"
                     /translation="MLDQLRKKQKVVIYIVAIVFILGMAAVGIGELLSPKPYLGKVNG
                     TKITYEMYQQKIQEMYERYAEMYKDQPIDENMRRNIENQAWETLVNDILWQQQIKKHK
                     IKITEDDVLTEMQNNPPQELMQNESLQTNGRFDKSKYLSALKNNPQFFAAMEEYVRGY
                     LPRQKLQEKIKAQAGITIDSLKAQYAKETDSVSGKFIWFEPNKSDPVTVTDAEIKAYY
                     DKNRETEYKKGPASRIKYLAFEEKPSDKDYASVKLLADELYNRALKGEDFATLANEYS
                     DDPGSKDNGGSLGVFGKGQMVPEFEQVAFALKVGEISKPVKTSFGWHIIRCDSIATIT
                     KDNPQIKASHILLKVEASDETKNAILDKAESAAKLIKKKGIDAAAKELGVEVQETDWV
                     PHDQDYIPGIGNNGALLQFMRKGKEKAISDVITDQQNRKIVAVLTDNKKVYYEDFEKV
                     KLRIKYQLEKEKKVANVKKKAEEFVAKYPSSEYFSAAAKEGWQIIELNNHKRDQSVPN
                     VGLCEEFTNAALALEAGQTSGLIHTKEGSFIISCTERIKPDFNAFAKDKDRQEQIRKT
                     LEDAAWNRWYDQMRKSAKIIDNRAKYGII"
     gene            44583..46169
                     /gene="secD"
                     /locus_tag="PLW08_01940"
     CDS             44583..46169
                     /gene="secD"
                     /locus_tag="PLW08_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPL51395.1"
                     /translation="MKKFSWRGLVIIIFFCVTAFFLAPMVIPNLPSWWSKHHLKLGLD
                     LRGGMQILLEVDTSELSAADARNAVEQNIKIIRERIDQFGVAEPSIQKLGENRIMVQL
                     PGVSDYKAAENLIKRTAMLEFKLVASQDEAKRVIETIDRNISSNLDKFPILAEMAKKD
                     KSIIADTTSTDSLKTGEGIFSSLIRPGEMDFEVQHSDVRLIQDLLADTLFAQMVPMGY
                     QLALDKADTENPRADRTLYVLSSAAQLTGGDLAKAKVEYGSATSTDPRIANKPYISIE
                     MKREGARKFERVTADNVGKRLAIVLDGVVYSAPNIQERIAGGRAQITGRFTATEANEL
                     AIVLNTGNLIAPIKPVSTSIIGATLGADSIRKGTMAGLIGLILVVLFMAIYYKLCGFI
                     ADIALVLNVGFILAMLTAFGATLTLPGIAGIILTIGMAVDANVLIFERIREELELGKT
                     PRSAVEAGYKRALVTVWDSNLTTVIAGIILYYLGTGPIRGFAVTLVIGIMGSMFTAIV
                     VVRTILDNMVLTGKRTKLSV"
     gene            46181..47164
                     /gene="secF"
                     /locus_tag="PLW08_01945"
     CDS             46181..47164
                     /gene="secF"
                     /locus_tag="PLW08_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPL51396.1"
                     /translation="MRILKNTNIQFVKSRYWFYGISIALLLISLCGIIFKGLNWSIDF
                     TSGIAATVNLKALDPKVPAVPIENLRTVLTKNGFPEAEITYIGNPANSTFQIKIKSVG
                     KGTDLSTETRTKLLEIIQQNFPEYVQGRDLNKEVIEEIYEVGPKVGSELRSQAFWAVM
                     LALIAMIIYIWFRFEFTFGLMGILALFHDVFIIVGIFALTGREMTMQIIAALLTIVGY
                     SINDTIVIFDRIREDMKKNRKDPIQVVFNRSLNKTLSRTIITGGTTFLTSACLFLWGG
                     SVIHDFALAICLGIIFGTYSSIFVASNLVIDLNLITHKERKTMQHLTKKKL"
BASE COUNT        14951 a         8552 c         9598 g        14650 t
ORIGIN      
        1 ccgctgccta tgctcagctt gccaatctat tcaaccgaaa agaatgggtc ttttccgaac
       61 agccgggaaa aggacgtaat ctctatatcg gcttgaaaca ccgtttttaa gattggacaa
      121 ggattatttc ccgcagattt gaataatagg tcttgaatga acaggtctat gaagatttta
      181 cggatttgga atgaagaatg aatttaacaa ggaaacttgg tagatttatg gttgagaagg
      241 ttgagagggt tgagaaagtt taaagggttg agagggttga gaaagtttag agggttgaga
      301 taccctttgc tctctgccct ttgccaaaaa gcccgtcagg gcgacacaag aatagcgatg
      361 gtggcgtaag cacctcgtca gattcacgat tattcagcaa agaaaatttt gactcacaat
      421 tttcacccgc cttccagaaa accacaattt actaactatc tcacttacaa cctcatttta
      481 actggtttcc aaattgaaaa attgtgaatt aaaatagaac acttttttcc gctctttttt
      541 ccttttcttt aaagttatat agatcaataa gatcccatat cattcccata tcgcgatatg
      601 ggaacgatat gggaatcata tcggaaggat atgagtgttg gctaagtgcg aaaagttatc
      661 ttaatgtgtt tctatatagt tagttctgat cttctgcagg aatggtttgg aatgtaacct
      721 caaattcctt ttcgggagca aaatcaaaaa attcccagat ataattcctg ccctcttgtt
      781 tctgcacgga gaaaggaaga gagcgtattt gcacatttat gcccggatgc aaagtatatt
      841 tggcgtaagg taagggtctg ccccaggaat ttgtagtggt gataatatat ttagcataat
      901 tgcttttaag ggtttgagtg taatcaaggt gcaaagtgca aaacgaaaga gcaggcagag
      961 aaaggagaaa agtgaaaccg ttctttgctt gatttagcat agttacttcc gctccattgc
     1021 tatcctgaat ggctatggct ttaattttag gcggtaggca taaactatca ctgggaacag
     1081 gaaaaaagat tagtttgtgc gtggaaagcg aatcgtaatt ggcaaaatga aatagaccat
     1141 ctatttccca taaattattt tccagataga aattcagctc ttccgcttca aaagtgagag
     1201 cggaaagcat cataatttca gctaataaca gcaggagaaa agcgtatttc acttaagtat
     1261 aataagttta gctgtcccca atgccttacc ttgatttgta agacgcacaa aataaatgcc
     1321 gctggaaagt tttggcagct gccattcaat ttcattttgg tcataaactt tttccgcaag
     1381 atggcgtcct ttcaaatcgt ataaaacaag ttctgccttg cctgtaagct tggtatcata
     1441 agaaattcgg atactttcgt tccaatgagg atttaataca gaaggatata gatggagttt
     1501 tcctgcagga atagaaacga tattttcttc cacactaaca ggcaattgca cagaaaaggg
     1561 aataagagct cccggttcag caaaaaactg agtattaaaa gctatttcat tggtaatagg
     1621 attatttacc caaatctgcg ttttcattgg ataaagctcc ccggtagcaa tccctgcact
     1681 attagtaatc gctggattta taaaaatacc tccggcggat tgactattat aaactgaaac
     1741 tccggcaaca ggtaaaccgc ttaaatcggt gcattcaagc ctgtaagtga acttggcaga
     1801 acaataattc ggtccgcaaa cagccggcga aacatctttt gcccaaccat aaacattgtc
     1861 accaataaga ttggaataaa gtatttgtac cgcacattga gttccattta gcggacttgc
     1921 atcctttcat tagaattttt gttcatttct ttctccttaa tattagtccg ctattttcag
     1981 gacacctcac aacttacatt ccctttgccc tctactcttc ttgcttcctt atttatattg
     2041 acagaattct gtaatttcca gaaaaggata cataaaacaa aagtgaagga ctataatgca
     2101 cttacggcaa gctaaggtga taaagagtat cttaaatgcc ctttttggcg attacaacgg
     2161 aattcaggtt ttcgttgccc ccataactct tttgtattgg atagatagtg gcagtttgct
     2221 atcttccgct accagtttgc ttagttttcg gatgcattat ttgcccttgc ttgccttttt
     2281 aattatcttt gtgttcagtg tttttatgct gataaaaatc aagttactat acaactgcaa
     2341 taatagcgaa tatttagata tggtaattca atttaatgtt agcgtgatgg ctttggtgtt
     2401 aatcggttta ataatttatg ccatttccag ttttctggcg tatttttacg ggataaaggg
     2461 tactgtaaaa tcgggtctgc ttttgttatt caaactctat accgtgtttc ttattcttta
     2521 tcattattta tttaatgtgg ttttaactcc ctattatcaa aagcagtatg gtcatcctag
     2581 agctttgaaa gcgtttcttt cttgggcacg gaacaataaa ttccttcttt tccgttatat
     2641 tttacttatt ttgctagtcg ttttctttgc tgtgcgtttc tatcagttaa tcctgcgttt
     2701 tgctctgatg ccgttaatcg gctttattga caaatatacc ggcatatcta ttaaattcaa
     2761 gctttatccc tttgttatga tagaagatat ctttgtgaat gtgttagtgc ttactggtgc
     2821 atttttggta tccaatctat ttttctttcc ccttatctgg gtattaaaat atttagttaa
     2881 tcgtttcata cctttcaaaa atctattgag gacaagctat gcccaaagtg cgtaaaaaga
     2941 aactgcgtaa ggttaagcgt cggagaaaag gcggtaatcc gggaattttt attctgacag
     3001 gattcatttg ctgtttcttg atatggataa tcataaaaga gcccgcggat aagaattccg
     3061 agctctctaa agccgaaacg gtttctaaaa acagcgaact accggttaaa ccaaagaaaa
     3121 aaaccaaaaa agaagtgaag gcaatttctc caagctcaac acagatatta accaaggaca
     3181 aaaaagccca ggaaaccact atagacaata caaaaaacac tccagaatcg gtaattacta
     3241 caaccattga aaaacttggc atccccaagt cctatttccg caaacgcaat acggaaaact
     3301 taactatatt caatgtgccc attgacaaat cggaaatgga cctcgtttat gctaatatga
     3361 ttttcaaagg tgaactggaa cgcaatggag ctaaatttat aaagggaacg gactcagcag
     3421 gtaaacaatc attgctcttc agttttcccg ataccgccaa acaatatgcg ataaatatct
     3481 tttatgataa taatctttat cggaacaaac agaaaaaacg cactattacc atcgttgtag
     3541 atgacttcgg aacaattggc ggtgatttgc ttaccggttt tctggctctg gataaggagg
     3601 tctgcttttc catctttccc gaagaagaat atagcgtgca aacaatgaaa ctggcaaagg
     3661 agcaaggacg cttaactctt attcatattc ctatggagcc cattggctat ccagaagtaa
     3721 atccgggaaa gaatcctatc ttggttcaat atgatgagcg gcaaattgaa aaaataatga
     3781 cccgctttat tgagcaactg ccttattgtg aaggagcgaa caatcatatg ggcagtttgg
     3841 caacaacgga tgaagaggtg atgaattctg taatggctat tttgaaaaaa cataataaat
     3901 tttttctgga cagtcgtacc accaatgttt ccgttgctta ttcggttgct caaaaaaatc
     3961 atctgaaatc atatcgcaat gacctttttc tggattctcc caatatcagt caatccacta
     4021 tggatgctaa actaaaccag ataatagagc tttccaatcg taatcaaaat gtgattgcta
     4081 ttacccattg ccataattac gataaacttg attacttaaa acgctttatc aaccgtctga
     4141 aagctgcagg tttcactttg attccccttt ccgatataga taaatataat gtccccgaaa
     4201 tactttagag gttaagttat gagcttaatt cagtcaattc tgatgagtat attacaaggg
     4261 ttaacggagt ttattccggt tagcagttcg gggcatttgg ttttagcaca acattttttt
     4321 ggcattggtg acagtgaaaa cattgttttt gaactcttta tgcacttagg aacactgctt
     4381 gccgttttgg tcttttttcg taaaactctc tgggagctta ttaagtccct tttttcctgg
     4441 ggaaatactg ttaatcggga acagcatcgt aaaaatcgca ctctggttta ttatctgatt
     4501 atttccactt tggcaactgg agtgatatat atcctgtttg gagattttat ggaagctgtt
     4561 tttaatatgc cgatgttggt agctatcctt ttgcttgtta ctggatttat tgtttttgtt
     4621 tccgattatt tcaaggataa aggtattcct gcttgcaata ttggttttct gcaagccgtt
     4681 ttaatcggta taggacaggg atttgccatt ttacccggga tttcccgttc cggaacaacg
     4741 attgcttgtt ctttggttac aggaatgaag cgcaaggatg cagctcaata ttcattttta
     4801 ttaagtattc cggcaattct gacaggaaat ctttcccagt ataaagcatt tgctaatttg
     4861 aagccgcaat tgctgataaa ttatttagcg ggttttgtct gttcctttct tgtcggttat
     4921 ctggttattg cctttttaat ccgtttaatt gaagtcagtc gtttgaaata ctttgctgtt
     4981 tactgttggc taattggctt gctctctatt gttttaatta tcttgggttt ttaatggaaa
     5041 caagtatatt tgcccttgat tttacagcgg ataaagtgcg tttaggttct tcctatttgc
     5101 ttattggaac agattcctat ctggtagata gagttttgga taccatcaaa gctaaactaa
     5161 aaaaaaagga aaaggtggat acttttatta tctatggtga tgaagtgaaa agtcctgaac
     5221 tggcagacct tctggatact tattccattt tttccgctgc caagcttatt atcattaaaa
     5281 atgcagaatc acttaagatc aaagaactga gtaccttagc cgattatttt tcttcgcctt
     5341 cgaaaatcca aagtttagct attgtaaccg aaaaagcgga tgctcggcac gaaaactgga
     5401 agaaaattaa ggcaggaact aaatttataa cttgtgaccc accaccctac ggaggagcta
     5461 ttcgtgcctg gttggataaa gcattgaaag atattaataa aaaaatgacc gataaggcaa
     5521 ttgaagaatt tctcaaccgc attgaacttg attattataa tgccgctaac gaacttacca
     5581 aattagattt gcttacccgt gaccgaacaa caattaccga ggaagatatt atgcaaagcc
     5641 tgggaactac acgcagcggg actttgattg atttttatcg tgctctggga agaaaacaag
     5701 caaaacaggc aatagaagca atggaaaaga tgctttttgc cgattggaaa cccttgcaaa
     5761 tcctttttca gcttacgcgt ttctatttca atatctggaa aatattactc ctgaaaaaag
     5821 ctcatattac cgataatgaa atcgtaagca agtatattac ggattttcat cctaatcagc
     5881 gaagagaatt tctggattta tcccgttact ataatattaa tgcgctggaa aaaatttttg
     5941 ccctcctact ggaaacagac caacagttta aattaaccgt tgccgagccg caaattctgc
     6001 tttgcaattg tttgcttaaa attctggatg cctaatgcgc aaaacaggta tcttactgca
     6061 tatcagttca ctgccttccg atttcgggat tggcgatttc ggacctgaag ccgtccgttt
     6121 tgccgaatac ctgaaagcag aggggtatac ttactggcag atattaccta taaatcattg
     6181 cggttatggc aattcaccct ataatccgct ttccgctttt gctttaaatc cttattttat
     6241 cagtccggaa ttgctctatc aacagggcta tatcaatcag ctgatgctgg aagcggtaaa
     6301 aattccccgg agcaatgtta ctcgctatga acaggtctat atcctgaagg ataaaatgct
     6361 tgctcaggca actgccaact ggatgcttgc caataggact gacgattata ttgaagaaaa
     6421 tgctccgttc ctgaaaccat acttaacctt tctttcctta agcaagtttt atgacgacac
     6481 ctgctggtat aactggaacg aagaacatcg tcactacagt gaaaagctct atcaaaaagt
     6541 ccgtttgatg aatagttctt atttcaatct gcgtgccgct actcaagcaa tgctggtaga
     6601 acaattatcc tggttcaaag aacgcttaca agaactgggc ataattttaa ttggggattt
     6661 gcctctttat cttgcttacg atagcgctga agtttgggct cacccggaat actttgactt
     6721 ggatgaacaa ggaaggcgtt tacactttgc cggagttcca cccgatgctt ttgcggaaaa
     6781 aggtcagctc tggggaaatc ctttataccg atgggatgta ttgaaacagg atggatttca
     6841 gctttttatg gagagaatca aacaagcact tggctatctg gatattttac gcttagacca
     6901 ctttatcggc tatgtgaatt tttggcaggt agagcccgag ctggacgcta atggtcaacc
     6961 agttttgcct aaagacgcta tcaagggttc ctgggtgcgt gccttacctg aagatttctt
     7021 ttccgctttg ttacaacggt ttagcataaa gcgttttatt gccgaagatc tgggtattct
     7081 caatgatgat gtttgccgta tccgtgagcg atatggtttt ccggggatga ttgttttaca
     7141 gttctgcttt gaagaaggcg ttcccgatgt gaaaaaatac cctcccgagc gttggcttta
     7201 taccggaact cacgataatc aaaccgtgcg tggctggttt gaatcacttt ctcccaattc
     7261 ttcgtcctat aaacacttac aagaatattg tgcactacat cagtttaccg attgtcaggc
     7321 gcatttgaat gctgaaaaca ttcatctcat tatgcgcaat attgctctct cttccagttg
     7381 ccaacaggtt attttccctt atcaagatat attgggactg ggagatgagg caagaatgaa
     7441 tattccagga accgctttgg ggaattggca gtggcgtctg gaaaatctaa atttctgaaa
     7501 tcaataaaat aaaaaggaga taaaatgcca aaacttatgc tgttgggtga cgaagccctt
     7561 gcccagggag cattggatgc cggaatatca ggtttttacg gttatccggg aactccctcc
     7621 accgaaataa ttgaatacgc acaaaggtct aaacaggcgg aagaaaacaa tgttcaccgc
     7681 acctggagtt ccaatgaaaa gaccgcactg gaaaccgctt tgggaatgag cgcttcaggc
     7741 aaaagagcaa tggtaacgat gaaacatgtt gggttgaatg tagcagctga ccctttaatg
     7801 aattcggcta ttacaggagc cagcggtggt ttaattattg ccgttgcgga cgacccttca
     7861 atgcacagct cgcagaatga acaggatagc cgctattatg gacattttgc gatgattcct
     7921 gttttagaac caagtaatca acaagaatgt tatgatctgg ctttttacgg ctttgagctc
     7981 tcggaaaaat atcatatccc tatcctgtta cgcttaacta cgcgtttggc gcattcccgt
     8041 tctggagtta gcagacgcaa tcctttaccg caaaaggaat tgaaaaagcc ggaagatatt
     8101 tttcaattta tgcttcttcc tgccattgcc aggaaaaagt atcaacatca tctatccctg
     8161 caaaaggact ttctggcaga atccgaaaat tccccgtata atgtatatac ggcagaaagg
     8221 aaaaatacca agttaggtat tatcactacc ggcttagctt ataactatct gcgggaagca
     8281 tatcacggtg aagaaattcc ctatccagtg ctaaagatat gccagtatcc tttgcccgaa
     8341 aaacaaatcc gtcaactcta tgagacctgt gacgaattgc tggttataga agaaggtatg
     8401 cccttcgtgg aagaacagct taaagggctt gcttttcccg gattgaaacc tattcatgga
     8461 cgtcttgatg gctatctacc tcgtgctgga gagcttaatc ctaatcttgt agctaaagct
     8521 ctttccttac cagatacaat aggtagacct gttcccgata ttgtagttgg caggcctcca
     8581 gctttatgcg ttggttgccc tcattccgat acttttttat ccctgaatga agtgatggcg
     8641 gaatatggaa gaggcaatgt tttcagtgat atcggctgtt atactttggg ctttatgcca
     8701 ccatataatt ccatcaattc ttgcgtggat atgggtgctt ctattacaat ggctaaagga
     8761 gcttccgata gcggactttt tcctgccgtt gctgttattg gtgatagcac tttttgtcat
     8821 tcaggtatta cggggctgtt ggattgcgtt tacgataaag ctaatgtgat gataatcatt
     8881 ctggataatg ccacaactgc aatgacaggt gggcagaact ataccggtta tggcaaattg
     8941 gaagatattt gtctgggatt gggagtggaa aaagaacata tccgtgtgtt tgttcccctc
     9001 aagaaaaatt atcccgaaat gatccaaatt tataaagaag aactggctta taatggcgtt
     9061 tctgttgtta ttgcccgtcg ggaatgcatt caaaccttaa agaaaaagaa caaaatggag
     9121 atacaagaat gaaaaaggat ataatacttg ccggagtagg aggacagggc attttaacca
     9181 ttgctactat cctcggaacc acagcaatca aacgcgggat gaatttgaaa caagcagaag
     9241 tgcatggaat gagtcagcgt gggggagatg tccaatccca tttgcgtctt tccgatgaaa
     9301 ctatttggag cgatttaata ccctttggct ctgccgatat gattttaagc gttgaaccgc
     9361 tggaggcatt acgctatcta ccctatctaa aagatgacgg ctggattatt accaattcca
     9421 caccttttaa aaatatccct aactatccgc cggaagaaga aatctacaaa gaaatcaaaa
     9481 aaatacccaa ctatgttcta ctggatgcag atactaaagc taaagaacaa aatgccagca
     9541 gaagtatgaa tatagtagtt ttaggcgctg ccattaaaca tctgggcttt agtaaggaag
     9601 aaattgaaga ggcaattaaa actatctttg cacctaaagg agaagcaatt gtagaggcaa
     9661 acctgcgagc tttgcaagcc gggatggaag gataactctc tcgtaaaata tcaattacat
     9721 taaaccaggt gttacaagtc ctcattgcta aataagtttg aggacagggt agactttatt
     9781 tcagcgatgt tgactcctga cttacagaga gcaaaggatt ttttagtcac tttttatgag
     9841 gagtttatgc acccattgct acaattatgt cgctcttaga acttttagct gagggcagag
     9901 agctaagagc atagatcatc tcaactctct caaaacctta tcatctcgct gtttcatttt
     9961 ttagcttgac aatatatact gtttgaccgt attgggtcaa ttgttcagtt taaaagctgt
    10021 aaccttcagg aagataataa aatataagga aggagaatga gtgctgctca gaacaaaaaa
    10081 aagttgatat tggatatagc tacccagcta tttacccgtt tcggctttgc taagacctcg
    10141 ttggatgaaa ttgctgctgc agctcagata gctaagggaa cggtatatta ttactttccc
    10201 agcaaggaag acctctttat tgcttcggta gaagccaaaa caagagagta tttcaaaatg
    10261 ttgaggcagc atatcaattc cacgactggt tttgaagaga agctgacgga atttttaagc
    10321 tttcccatca aactgattta cgaaaatatg cccatcctga ttgagtgttt aattaatata
    10381 ccgcaaagtt atcaggaaca gttgattcag ttccgagtta aaagcaggga agtgatgatg
    10441 caaattcttt ctgaaataat ccagcttgga aaacaggagg atttaattga tgagcgcttt
    10501 ccggaagacc gtctttgcga gataattatg gattggttta tgataggtga accgagtttg
    10561 aaaatagtag atgttccccg cttaatgaca atgattgagc gggatagtga ttttattatt
    10621 aagatgctac tttatggaat tgttaaacga ggataaaaaa tgagaaagac attaattgta
    10681 ttaacactgc taacattatt agcaagctgt gtatttgcca ttgacctgga aactagcatc
    10741 accaaagcta aacagaataa caaggaactg ttaatggcaa tggaagaaat taagaaagcg
    10801 gatgagtcat acaaacaggt tcgtggcact gcttatccac aattgaattt acagggtgct
    10861 tacggtttaa gtaagaccta ttatcctgat tcagctatac ctgctaagat gaatatatct
    10921 gccggtttag atgatacagc aacagataat gaagaatatt tagcgggtgt actgaataaa
    10981 atagttaatt caatgattcc ggaatcgccc tcagaaactg gttcttttgc tttacagtta
    11041 aaaatggaac agattgttta ttccggaggc aaactgggaa gcggaattaa agcgggaaat
    11101 tattaccgcc aaattcagcg actgaattat aaagtaaaag aacaggatgt ggttttgaaa
    11161 acaactgaac tattttatca gtgcttactg gcaaagaaac tgtgggatgt gcaagaagag
    11221 ggcttagata ttgccaaaag gcatttgcgc agagtagaac tattcaatca ggaaggtcag
    11281 gtttcggaat ttgacttgtt acaagccaaa ttaggtgtag ccaaactgga gccccaggtt
    11341 ttgaaagcaa aaaatgatta tgatttggct gtttcagctt ttcgtaagca aattggagaa
    11401 gaagagagtg atatagttcc cgaaggtgaa ttcgtgttgc cggaaaaaat ggagctctct
    11461 ctggaagagg caactgaaca gggtttgaaa caaagaaatg aagtagaact gattaaattg
    11521 ggtacccagt taaaagaact gcaatataag gtagaaaaag tgaatttcct gcccaatgtt
    11581 gccttgagcg cagattattc actttataca gcggcagatg aatatgctat tgagaaagat
    11641 gatttcgggc ataaatacgg cttcagtttg ggctttcaga ttcctttgtt taccggctta
    11701 accaatacag ccaaaagaaa ttatgcccgc tatgattatc aaaaagccaa actgcaacaa
    11761 agagatacgg aagaacttat tgctctgcaa attaaacagg attatcaaaa atattatcac
    11821 gcttgggaaa atcataatgt gcaaacagaa aacattcgtt tggcagaacg cggtttacag
    11881 cttgcccaag tgcgttatga aaaccaggtt ggcattcagc ttgaggtctt tgacgcccaa
    11941 ttaaccttac aaaccattaa actgcaatat tatcagtcaa tttatgaaat catatctgca
    12001 gatagaaact ttaaaaaatc aatcggtata accctataac ggagaagata aactatgaaa
    12061 agatacttaa ttattctaac agccctgata ctgatactta tctctgcctg cggtaaaaaa
    12121 caggaagagg gaaaaagtat ggaacaaata tatagcgaag aaggggttcc cgttcgccaa
    12181 attattatag agcccacaac cttccgtcaa aacctgcttt ataatgctac tttaaacgga
    12241 atggaagaga caaccgttca ggcaatggtt tcggaagtga ttaccaaaat aaatgccaag
    12301 gtaggggata gggttgcaaa agaccagatt atcgtaagtt ttccccaaaa tacaccttcg
    12361 gcacaatatg aacaagcact aacggcattt aacagtatca aaacgactta tgaacgaatg
    12421 caacggcttt ttgcccaagg tgctatcagc cagcaggatt tggacaatgt agaaacccag
    12481 tataaggtat ctaaagccaa tctggaaacc agtgataaaa tgatcaatgt gcgtgctccg
    12541 ataagtggta ttattacagc aatatatgta aacccaagcg agaaggttta tccgggaaaa
    12601 gacctgttta ctattgcatc taccaatggc tataaagcta ctattatggt tcctgatacg
    12661 gaaataaaca agattaaaaa aggcactaaa gcaactgcta cctggttgga aactaccatt
    12721 tcaggaagag taacggagat tcctctggct atggataatg caaccaaggc atttaaagtg
    12781 gaagtaagtt ttccgggaat aaataaaaag attaactacg gagtaacagc tgaaatttcc
    12841 atagaagttt tcagcaaacc caatgtgatt gtggttgagc gccatcaaat cgttagtgaa
    12901 aacggaacca aatatgtttg gctgaatcaa gatggtaaag ccgtgaaaag agaaattacc
    12961 acgggactgg ataatacgct ggcttttgaa attaccagcg gtttaaatcc gggtgatttg
    13021 cttatcactg aaggtatcaa tatgttaact gaaggcgcaa aattgcgcgt tattgagtaa
    13081 gggaatagga actatgtttt taacagatct gtctattaac cgaccagttc tggtcactat
    13141 ggcaataatg gtgtttattg tcttcggagt tcttgcctac ttcagtttgc cgattaacct
    13201 gatgccggat ttgaagttgc cttatgttgt tgttcagacc gtatattcag gagccggtcc
    13261 ccgcgaaatt gaatctcagg taaccgaacc gatagaagaa gcagttgcca cggtcagcca
    13321 gattgacttt atgcaatcct actcaatgga gaatgtctct attgtgctgg ttgccttcaa
    13381 attgggtaag aacatagatg ttgccaatca ggaagtgaag gataaggtgg atgccgttat
    13441 ccgtaattta ccagatggca cggacagacc catagtcctg aaaatggata ttaccgcttt
    13501 tccgattatg gacctggtgc tttccggaaa tatgcctccg aaggaattat acgagcttgc
    13561 ggatgggacg ctaaaagatc gtttatccca aattcctggt gtagcaagtg tctcgttaat
    13621 tggaggagcc aagaggcaaa tagatgttcg gtttaccgat aaggttgtgt ttgaaaataa
    13681 aatatcactt gctcagttaa gtgcaattct tgctgcagca aatttggata tgccgggtgg
    13741 taattttacc caaggaactc aggagtattc agtccgcttg aaaggggaat atcaagatgt
    13801 ggaagatatc agaaataccg atatacccac tgtttttgga gtgaaaaaac tttcacagct
    13861 ggcaagagtt gaagatgcta cagaagaagt gcgttccaaa gcaatttatt ttaatgttcc
    13921 ggaaaatgtt aaagatgaaa atatagtccg tctgtctata accaatgctg ccgatggaaa
    13981 tgttgtaact atcgctgaag aagtcaccaa agaaattccg attttaaata aagaacttcc
    14041 ggaaggtgta aaactggaaa ttatgcggga tgatagcata tttaccaaag atacgattaa
    14101 ttctaccctg gaaaatattc tcttgggtat tctctttacg ggacttattc tcttcatctt
    14161 tttacacgat ttccgttcta ctattattgt agcaatatca atgccctatt gcataatttc
    14221 aacctttgtg tttatgcaaa ttttcggcta tactttcaat attatgacga tgatgggtct
    14281 ttctaccgca ataggaattc tggtttccaa ctcagtggtc gttttggaga atattttccg
    14341 gcataaagat atgggaaata ctagaaaagt tgcagctcaa attggaacca atgaaatagg
    14401 tacagcagtt ctggcatcta ctttaaccaa tattgtagta tttttaccta ttgctacaat
    14461 gacttctatg gtgggacgct tcttcaagga gtttgctgta accgttacta tggcaaccat
    14521 attttctctt ttaaccgctt ttacgattac acctatgctg gcttccaaga ttattccgaa
    14581 agagaaacgc accattaaat ggggaaggaa atttgataat gtctttgata aatttggtaa
    14641 ttactatgcc aaattcttag gatatacatt gcgttccaag aaaaccagtg ttagtatcct
    14701 tctttttact ctattggctt tgctgattac ttttggcctt gctttctttg tgggtatgga
    14761 aatgatgcct cctgtggacc aacgcaatct atccatttct gtagaaatgc cccagggaac
    14821 aaatttggaa cagacagcta aaacaatgga catcatccaa aaccggctat cccaacataa
    14881 agaagttgtc cacatactca ccaatctcgg ttcaggcagc tttattgata ccggcaccaa
    14941 tttggctact tccgatatta aactggtgga taaaaatgaa cgtaaatata ctgctaatca
    15001 attagcagat atactaacca aagaacttgc cgatattccc aacgccaaaa ttaaagttac
    15061 cggctcaatg ttaggtatgg gaggaggtgg caggtctggt ttgcaattta tgattgaagg
    15121 tcaggataat gcccgtttgg aacaactgaa aaacgaagta accaaagcta ttgaagatat
    15181 accgggttta atcaatctgg atacttcaag ccgttatggc aggtctgaac ttactcttta
    15241 tcccaaacgc gataaacttg ccgctgccgg agctacggtt tatgatttgg ctttagcttt
    15301 acgcgctaat gtggaaggac ttgtttctac caaatatcgt gaagccggaa atgaatatga
    15361 tatcaagatt tctcttgagg atgaagtagt tgatactccc gataaaatta aaaacctgag
    15421 tgtagttgtg atgggtaaat cctatttggt atctcagctg gcagatattc aattttcttc
    15481 cggtattaat agaataactc actacgaccg ctataagacc gttatgttta ccggtgatgt
    15541 agccagtgga tacactatga gcgatataac ttcccaaatt gaagaacgat tggataaaat
    15601 taaatttccg accggctatc gttatcaatg gggaggtcag gcaaaaatga tgagtgaaac
    15661 tacagtagat atgattcgca cttttattct tgctgttatt ttaacctata tgctcttggc
    15721 ggctattctg gaaagttttg ctcaaccaat tctgattttg gcaacggttc ctctggcttt
    15781 gattggagtg atactttcct tactcattac aggtaataca tttaacatcg tttctatgat
    15841 ggctataata atgttagttg gcattgtggt aaataacgct atattaataa tggactatgt
    15901 aaatattaaa cgcagagagg gctatagtgt ccacgatgcg ttgatggaag cgggaaagat
    15961 gaaattaaaa ccgatagtta tgtctacctt agccatcata gcgggaatgc ttcctatggc
    16021 aattggtata ggtagtacgg gatccgaaat gacccgtccg atgggtattg tgtctattgg
    16081 tggtttaatt gtttccactt ttctaacgct gattattatc cccgctttct atttcctgac
    16141 cactaaaaat atccatgcta aaatggaatc ccaataaacc caataaaaag gagagaaaaa
    16201 tgaaaaaaat cactcgtctg gtctctatcg ctttattagc ggtatttatc gttgttagcg
    16261 tcttcggatg ctatgggaat atgtcactta ccaaaaaagt gtatcgcttt aatggtagtg
    16321 tcgggaataa gtttgttcaa agcattgtta actgggcttt ttggatttgt ccagtttatg
    16381 aagcagcaat gtttttggat attgtgattt ttaatactat tgaattttgg accggtagca
    16441 atcccctcgc tatgaacgaa ggtgaaaata ttatcaagta tgctgaaggt cccaatggga
    16501 attataagtt tgaaatcagt caaaacaaaa ttgtaatatc tcaacctgaa aataaggaca
    16561 acaatcaaaa tattgaactc aactatgacc ccgttagcga atcctggttt atgaacagca
    16621 atggaaccac ccaaaaaatt ggttatttgg atggaaatca attaaaattg ctttctcaat
    16681 ctggtgaaga aatctcttta gacctctcta aataaggata aaaagtgatt gcaaaagaaa
    16741 aaattgaaag tatccttgca aaagttcgcc cctccattca agcggatgga ggggatgttg
    16801 aacttatcaa tattcgtgaa gataatgtga tagaagttcg gcttaaagga acttgtaatg
    16861 gttgccctat ggcaacttta accttgaagg caggcattga acgacttatt aaagaagaaa
    16921 tcccagaagt aaaagaagtt atcgctgtgt aacttcccag aaggaaagtc agtagtcatt
    16981 agcgccttga taacttctta tatgggagat aactttcttg gcgcttatga ctcgtgacga
    17041 cagtgacaat tccaataaaa gcattgaaat atgcttggaa caagttgtaa cgagtcctca
    17101 ctgccaaagg aatttgttgc ataagcgata tttttaaggc agtgacgatt cgtgacttgg
    17161 ctattttttc aaatatgcat tgactttttt gttagctttt tggaaatata aaaaaataat
    17221 ttttgtttaa ctaaatcctg agttaaagta ttattaaacg aaaaaaggag aaacaatgaa
    17281 gtccatagtt aaagctctaa tcctcttatt accattcctt ttttgctcgt tagcgatggc
    17341 tcaaacgaag agtgacagta ttttcgtatt cttgaataaa caggattttc ccattgccag
    17401 cgtggataat atgttcattc ctattagcaa tccttctttg ctgggaaccg gttctgcttc
    17461 gggtataggt ttagcttatc tcaatgatga aaaagaatgg caaaatcact attggatatt
    17521 tttgaatact gatttgctca gttatattta tgaacgcgac catagttata aatatcatac
    17581 tttagctttg ggagctgaac tatttcctgc acatatttta cccaacttat atgcaggaac
    17641 gaattaccgt tggcaggaag gcggttttga agatggttct ttcagaactg gagttacata
    17701 ccgtccccat aattctactt ctttagcctt tacatgggat aatcccaaac atcaatcaac
    17761 ctattatcgt ttaggattgg cagtgcgtcc ttttgttttt acagatgcta tagcggatta
    17821 ccgtttggaa ctttctgtag atgctaatta tgcgcgctat gaaatggaca aggattatga
    17881 attcaaaaaa ccggttctgg gaattcagac ccaaattttg gatggagtaa aagtcggagc
    17941 tacctataat tgggaagaag agtcagcttt agttaatttc agcatatgtc ccagaaatct
    18001 tgaggcaggt ggcttattgc gttctaagaa gaatgataat tatggaattg cttgggctca
    18061 gcttacagat tgggagcata aaccattttt gggacataca aaaccctcct ggtataaaat
    18121 ggatttgaaa ggcaacattg taacatacac tgctcccaaa tatgcaatag ggaaaatcaa
    18181 aatatacgat accggcgata aatccataga gtcagtcatt gataacatta aacaggcaaa
    18241 agatgatccc gaaatagaag gtattcttct gaaaaatcct tccttttcaa cttctttagc
    18301 tttacaggaa gaactcattg atgcctttaa cgactttaaa agcagtggca agaaagtttg
    18361 tttttactat aataatattg gcaatggcgg ttatatcttt gcttcctcca ttgctgataa
    18421 gatttactta aatccgacgg gttctgtgga tttacggggt ttaagtatca gcagtcctta
    18481 tttgaaaaat atgcttgcca gcttgggtat tgaagtactc aatttccgca gtcacgaata
    18541 caaagatgcg ggtaatatgt tttccgaaga aagaatgact cccgcggaaa gggaagctta
    18601 tgaatccctt ctgcaatcac tatacgacca aattttacaa agtatggaaa caggacgcgg
    18661 tgatagatta gttgcttcag ctgacgaaat tatcaatggc ggtccctatt ttattgctaa
    18721 tgatgctctg gaaaaaggat tagtggatgc cctcatttat gaagaccagc tgaataaaca
    18781 attaaagaaa gattttaagt tttcaagtca gcataaagaa ttaactgaat atcgcgaata
    18841 tgcttgggca aaacccaaag aaaatttggt cgcggttatt tatgctagcg ggaatattgt
    18901 ttcgggaaaa ggaactccag gacaaaaaat tgctcaggaa acaacggttg atcttatccg
    18961 caaagcaaga aaagacaaac aatacaaagg aataatttta agagtggata gcggtggcgg
    19021 aagtgctcaa gccagcgata ttattttaag agaactggaa ctggcaaaga cagaaaataa
    19081 aaaactgatt gttgtttcta tgggaggaac agctgccagc ggtggatatt atatttcctg
    19141 taatgccgat aaaattattg ccgagccctc aactttaacc ggttctatcg gtgttgtggg
    19201 attgatgttc aatggcaccg agatgtttca aaaaataaaa gtaaactggg ataccgttaa
    19261 aaaaggtgaa cattccgata tgggttctat ttaccgtcct tggactgaag aagaaaaaca
    19321 aatggtaact cgcagtatag aaaattgtta tgatgacttt gttaagaaag tggatgaagg
    19381 acgtccgaat atgactttgg aacaggttaa acaatatgct caaggaaggg tctggacagg
    19441 agaacaagca caaaaaattg gacttgttga tgaacttggt ggattggaaa aagctaagga
    19501 aagtatgagt gaacttatcg gtaaaagagg aaagttaacc ctcattgatg ccactactaa
    19561 aaaagaggga gtaaaaataa gcattaatat cggtgaattg aatgttttta cacctgttaa
    19621 agcaatgaat gctgttaatt ctgattatat caaactctat gaattgtgga gtgattttgg
    19681 acaggataaa gcactgatgc tatgcccggt tattccagaa acattgcagt tttgaataat
    19741 aaatctggaa tgaacaggat tgaaaggttt gagaaggttg agaaggtttg aaggtttaga
    19801 taatctttgc cttttgggct ttcaaccttc tcaaccataa atctaccaag tttccttgtt
    19861 aaatttattc ttcattccaa atccgtgtcc cgttccttta aggttcaagg tttttgagca
    19921 acaatcagaa ttcctgttcc ttgtttgtta tctgtcttca tatccaaacg cattaggatt
    19981 tcagctaatg gataaacaag aagataatac aaaggcagaa gaagataaag ttttttagaa
    20041 atcagctgga ggggtatttt aattaataat ttccaggata aagcaccata agttccataa
    20101 gtataaatag atttaataat tcggaaacca atccggttca gtttattttc cagttccttt
    20161 ttattatagc cagcacgaat gtgttcggaa gtaaattttg ctgtttcatt taggtctgac
    20221 ggagtagaaa gaagcaaata tccctttttt ttcagggcat tatacaaatt attcagggcg
    20281 gaaacatcat cttcaatatg ttccataata tcaattgcta aagccaaatt aaacttttgg
    20341 gaaggagtaa aagtttgtaa atcggctgtt ttataagtga acctgcctgg ataataaata
    20401 tccgcgtatg cagaaaagga tcttaaataa tctgttttca ggtctgtagc aaaagcattg
    20461 gaattgagcc aatgcgatag aacataagcg gaatattgac aaaagccagc tccggcatca
    20521 tataaattaa ttttatccgt ttcccggaac aaattgcgaa tctctttcag cacatatctc
    20581 tggcggagaa aaataaggtt aaaagccata taaaaaagtt tgcgcaaagc aggaaacata
    20641 tctatagctc ttgccagacg gttttttaag gggtcgtatt ccatttcaca ttctcccgat
    20701 aaaaaaaata cattgactat ttttagcaga gccaaaataa gtcaacccag aatttgaatt
    20761 atgatagtaa aagaaataaa tgacattcag cgtgttaatc tgcatttgca tactaatgtg
    20821 tccgatggaa atataagccc taaggaactg ataaaacgct ctcaggaaat tggcttggat
    20881 ttaatttcca taactgacca cgatactgca gatgcctata cgcaaattcc agataatata
    20941 ttgcctctca gaatcttacc tggaatggaa ataagttctc tgtataaggg tgatgatgtg
    21001 catattctag cttatggctg tgattttaat aatcctgctt tacgagctat gacagagatg
    21061 tatttgaaag gcagaaaaga acgcgcccaa aaaataatta aattgctgac agatatgggt
    21121 atgccaatat ctttggaaga agtaattgct attgctggaa gcagagaatt aattgtgcgt
    21181 cctcatattg ctcaggtttt ggtgaatcat aagttttgcc aaacgaggaa tgaagcattt
    21241 gaaaaatata ttggagacaa caaacctgct tatgttccca agccggaggt ttctgtttct
    21301 gatgttattt ctattattca tcaggcagat ggcttcgctg ttattgctca tccgggaaaa
    21361 ctggcaaaac tatcctatct ggaagatatt attacaatgg acattgacgg tttagaggtt
    21421 tggcatccag accattatca gtgggaaata gaaaatttca tcaaaattgc aacggaaaaa
    21481 ggactgtata tgacaggagg aagtgattat cacagcgaac aggataaaca taatttgttt
    21541 gatattattc ctctgccgga agttgtcctc accagcgtaa aaaaactttg gaaggaatat
    21601 caatgccgcg tgaaattaag gtaaaatcaa tccgtaacga attgccagcc acttatcgtt
    21661 attctccctg gacgagattt attatgttat tcgtggcttt ggcaatttgt atctatgcaa
    21721 tctttttctt cgtaaaattt gtgactggca ctacaccaat gttttttaag gttctgcctt
    21781 taatcattct gtttgtagcg ttggattcgg tgttcaaaca acttacttct ctttatagcg
    21841 tgaccttcta tgaagataga atatgttttg gatttttatg taaacccaaa atcattattc
    21901 catatgaaaa tataacttcc attgatttga cgaaacagat ttccttcagc attattttga
    21961 aatacaaaga taaagacagg gaaaagatat tcaaaacctc agcttcattt cctaaaatcc
    22021 tggagatttt gcttaacctg gctgattttg ctccccaagc tcaattgaat gatttattgg
    22081 gtaaagttgt tagcaattta agaaatttag cgggaaaaag tgaaacagag caggtttgat
    22141 gaaataatag accgtcgtaa tagcggttgc tataaatacg atggtctgaa aatgctgtat
    22201 ggcaaaagtg agcttatccc tttatgggtt gccgatatgg attttgctgt ttccgatgag
    22261 attatttccg ctttatcgga gcgcttgaaa catcctgtct ttggctataa cttgcgtctg
    22321 gatgattttt atgaggcaat tatttcctgg caggaaagac gctttgattg gaaggtgcaa
    22381 aagaactgga ttatagctgt tcccggtttg gttcctgcta tttcattagc tgttctttcg
    22441 ttaactgaac ccggagaggg aattcttatt cagacccctg tttaccgtcc tttttatgat
    22501 gctgttctgg accataaaag aaaactttta acctctgctc tggtgaatac aaatggcatt
    22561 tatagtattg actggcagga ctttgaagag aaattgaaac aggcaaaatt atttattttc
    22621 tgtagtccgc ataatcctgt aagtagagtt tggacaaaag aagaattaac taaaataggc
    22681 agtttatgcg ctaaatataa agtccctgtc ttttccgatg aaattcatgc ggatattgtt
    22741 tatcctggtt acaaacatct tcctttggct tcattgaatg atttttccga aatttgttta
    22801 acaggtgttt ccccttctaa aagttttaat attgcaggtt tggcaacggc agttgtaatt
    22861 gtttcaaatt cagaaataaa gaataagttc aatgagctta atacaaaact tcatctctat
    22921 cttggtaata cctttgggat aagagcgtta attgctgcat atagtgaatc cgagaactgg
    22981 ttggatgaat tactttgtta tttagataag aatagaatat atatcactga atttgtgcag
    23041 aatgaattac atggagtgaa aataagccct attgaaggga cttttttagc ttggctggat
    23101 tttagaaact ggggtttgac agaggcgaac ttacaagaaa ttatagttaa taaagcggga
    23161 cttgctcttg aacccggaac tgaatttgga aaagacggag aaggttttat gaggttaaat
    23221 ttcggatgtc caaagagcat tttagagatt gccttaaagc gtttacaaat tttagcaagg
    23281 gaatacggat gaaacaggaa ataattgaac tttataataa cccaccggaa aaatggacag
    23341 caaagcataa agcattattt gcagctttta tagatgcctt aaataaagga gaaatccgct
    23401 cctgcgaaaa agaaaaaagt atttggaaag tgaatgaatg ggtgaaaatg ggtatccttc
    23461 ttggctttcg gatgggggaa ttaactatct atcaatggag tgaagcgaaa cctttttttg
    23521 ataaggatac tctaccggaa aagcaattta ctttagcaga tagaatcaga attgttcccg
    23581 gaggaagttc tgccagaaat ggttgttata tttctgccgg agtaacgatt atgcctcctg
    23641 cttatatcaa tatcggtgcc tatgttgata gaggaactct tattgattca cattccttgg
    23701 ttggttcttg tgcccaaatt ggaaaaaatg tgcatctttc cgctggagct attattggtg
    23761 gggtattaga acctgtggga atgcgtcctg ttattttaga agacgatgtt tttgtaggtg
    23821 gaaacactgg aatctatgaa ggaataattg ttcaaaacaa ggcagtaatt gcttccggaa
    23881 ctgttataac cgcctccaca cctatttatg acagcgttcg ggaaaagttt ttagaacgcg
    23941 atagcggcaa tagttttacc attccttcta aagctgtagt agttccaggt tcacgacgac
    24001 tgaaaagtaa tcctgatttt caaattgcct gtccgattat tataaaatat agagatgata
    24061 aaaccgataa agcagtagaa ttggaacagg cacttagaat ataataatag gtttgggatg
    24121 actatcctga aagagcagag agcggagaga ttctcaacct tcttctctac ctatcatcca
    24181 ttgcaatccg ggtaaattcg ttttatccgt aaaatgcata ttgctaataa atcagtgaaa
    24241 gatattgact attccttact taattattag gtttcattta caaaaacaag attattctgg
    24301 acaaaaagac ctgctttttc caatatgaca aaaaaagata tatcataagg agtttaaaga
    24361 tgaacagctt aggtttcaag tttcctgttc aggaaagaaa aacagccctc tacaatttgg
    24421 aagaggaatg gtatcttccc cttttatcaa aacatctcgg tattccaatg caaagcatca
    24481 aacgcagcaa tttcggtcaa tattctatgg cggtaaatta tttaacttct gtgttagaag
    24541 aagcagctgc catcaataaa gtagctgtct ataacattga ccatatggca caattactct
    24601 tttacggacc tgatgtagta actcttttaa atcgggcttt aaccggaaat tttaccgaca
    24661 tgaaagttgg acaatgtaaa tatactttac tattgaacga acagggtggt gtgcaagatg
    24721 atatgattct gatgagagtt actgatacca gttttattct ggttatcaat gctggacatg
    24781 atattactga tacaattgaa gtagatggcg aaaaacaaga atatcttgct gatatagaca
    24841 gaattatgca atgcaaaaaa gaaggggaag aggtttgggc aaaagatatt tccgatacct
    24901 tgctaaaaat agatgttcaa ggtcctctat cttacaaatt gctaaaagag gtctatggtg
    24961 aaagtgtgtt gaagaacaga aataatccgg aaaagaatat gaatttcttc acttttaatg
    25021 agtttgaacg caacggacac cattactatt tatccagaac aggatatact aacagatggg
    25081 gttgggaact ttatattcct gttgccgttg ctgaagaaga tgccaaaatt attatttcca
    25141 aggcattgga cttcggagga ctcttagttg gtttaggtgg tagagatgaa aacaggattt
    25201 ctgcaggacc tttcggttta cctttgatgg ggcaggaata tgacccttat catacacctg
    25261 taaatgctcc tctttttgaa accgcggtag atatgaacaa ggaatacttt gtaggaaaag
    25321 aggcattagc caaagaaata gcggaagggg tgcaaaagcg tttatatatc tttgtttccg
    25381 aaggtattgt atctaatcgg ggtatttata aagatggcaa acgcctggga actgttacca
    25441 gcagtataaa ttctcccaat gtttcgtggg aaatgcgttt agcaattggt tccaagcgta
    25501 aaaatgtgaa tgaagaaaaa ggaactgctg ctatcggaat gggttggttt tattctccat
    25561 tgtttgaaaa agatgatgaa ggaaaagaaa taggggaaat tgatggtaaa ccaattcgta
    25621 taccggttga attctaccgc gaggatgaaa accgtaagcc gacaggaaca cctgttttgg
    25681 gttatgttac cctggaagga attacaccag ctacagcaca taaaccgctg aagaatatag
    25741 agaacctgtg aaaagtatgt agccggagga cgttggtcct cctactacac aaaacggaag
    25801 aacgacatct tgtcgttcca cttataatcc agttttttct ttaacgcgaa cttttgagta
    25861 acgaaataaa ctgcaagaat ttggttgacg aggacatcaa ccttccgtgt ttactttact
    25921 atgatttcgg ttgtggtggt aggaggataa tttccattta catctaagga atccttatct
    25981 accataataa ctttattttc tacctttctg tctaagtgaa cttttgtaaa aggaggattg
    26041 attttttcca catggagaca ataacttaca atcaaacccg aaaagacaat agaaatcata
    26101 ctcatcagct ttaccagaat attgatgcag ggacctgcag tatctttaaa tgggtcgcca
    26161 actgtgtcac caacaatact ggctttatga atttcagtgc ctttagcttt cttttccgtc
    26221 tctatatact ttttggcatt atcccaagcg cccccagcat tattcatcat cacagctaaa
    26281 acaaaaccgg tggaaagggc tccagcaagg atacctaaaa ctcccggaac accaaaaatt
    26341 aaacctgtaa tgataggaga taagagacct actaaagcag gtataagcat ttctctctga
    26401 gctgcctgag tagatatttt aacgcatttg gcataatccg gttttcccgt tccttccata
    26461 attcctggaa tttctttaaa ttgacggcga acttctttta ccattttacc ggcagctctt
    26521 cctacagctt taatagttag tgatgaaaat acaaaagcaa ccatagcccc aatgaaaaca
    26581 cccagcaaaa atttgggatt taacacattt atttggaaga attgaataaa atcggcaatt
    26641 gatgcctttg ctaagggaat attttcagtg aatttggtat tataattagt atgcaaaaag
    26701 acagctttcc ctgctttagt tcctactaaa tgtaaagctc cgcgaacttc ttccagatag
    26761 gcagcaagta aagccatagc agttaaagca gcgcttccga tagcaaaacc ttttcctgta
    26821 gctgcagtgg tattaccaac ggaatccagg ttatctgtca tcattctaac atcttcaggc
    26881 aaattacaca tctgtgcatt tccacccgca ttatcagcaa taggaccaaa ggcatccatt
    26941 gccaaagtta cacctaaagt agcaagcatt ccgaccgctc caaaaccgat tccatataaa
    27001 cccatttcaa tagaagaaaa accgtgactg caaataaaag cagataaaat gcctattaca
    27061 attattaaaa caggagctgc tgtagaacgc attccaacag aaattccttc cagaataaca
    27121 gttgcaggac cataatctgc ctgattagct atttcccgtg ttggtttata gctatgtgaa
    27181 gtgaagaact cggtaaaaag tccaattaaa attccagctg ccaatccaat taccgttgaa
    27241 ataaataccc caaaataata atcaggggga agcaaagctt tagttacaaa gtaagcacat
    27301 atagcaatca gaatagaact ggaataaaca cttttattta aagcaaacat cagttcttta
    27361 gtacccgcat tttctttggt gctgacggtg aaaataccta taatagaaag cagaattccc
    27421 aaaccggcta aaatcattgg tgctaccaca aatttcattg cccagtcggc ataagcggga
    27481 cctaacatag ttacagcact cattcctaag gcagctgtag ccaaaataga accggaataa
    27541 ctttcatata aatcggctcc catacctgca acatcaccaa cattatctcc aacattatct
    27601 gctatggtag caggattacg agggtcatct tccggaatat ttgcctcaac cttaccaaca
    27661 agatcggcac ccacatcagc tgctttagta taaatacctc ctcccaaacg agcaaaaagg
    27721 gcttgtgttg aagctcccat cccgaaagaa agcattataa cggtaatttg ttgcagaggg
    27781 aatttaagca gattgagaat aaaaaaccag gcagatatat caattaaagc cagacccaca
    27841 acggttaaac ccatcacact tccagctcta aaagctatcc gcaaaccttt attaagtcct
    27901 ttagtacatg cctgagtagt tctattggaa gcaagggtag ccatattcat cccgatatag
    27961 ccagctaaac cactaaaaaa gccaccggtt aaaaaagccc agggaattaa ccaatgtaaa
    28021 acacccaatc cccaagccat tatttgaaaa acgctgaaag caatgataaa gaatatacct
    28081 acacctttat attgttgctt taaataggca aaagcacctt ccctaacata acctgcaatt
    28141 tcctgcattt tagcattgcc agggtcttgt tttttaacct gacgaaagaa aattgctgca
    28201 aaaattaaag ccagaatagc acctaagggt gcaataatcc acaggaaagg gatattatta
    28261 tgcatataca acctctaact cattattttc aaaaaagcta tgtataattt ttattgcttt
    28321 tgtcattatg tgaatttgca aaaaattgtc aatgaaaaaa gttgtgtgcg gtcataaaaa
    28381 attaaacctg gatgctttta tggaacgcaa caagggcaaa agcaggaact gttcccgatt
    28441 ggtcaatttc ctaccatcca ttttaattct ttgccaacgt gtatagggta aactgttatc
    28501 accaagacca tagctgattc caaaagtagc agagagctga aaatttttaa tcaatcttgt
    28561 ttctatttca ttttgggcag ggcgaccata aggataggca aaataacgct tacaaggcac
    28621 ggataattct tccatttgtt taaagcacaa atcaagctcg gtatatgctt caggataaat
    28681 taaacgcgaa aaatctggat gagaccaact gtggatacca aattcaacac cggctttttg
    28741 taaacttttc atctgggcaa gcgaaagaaa gggtttatgt ttttggagat aatcgctttc
    28801 tgaaaaaggc ataacttcct tccaaaggga atcggtaacg gtatcttttt cagacatttc
    28861 aatgcgggaa aaaaaagatg atattgttgc tccgggaaca taattgtcta tttgcgctga
    28921 aagttttacc ggcgaacaat agcgtttcat taaaagcaat ttatgattcc atgccatttc
    28981 tttgttatca acgcatttac caattaaaaa aacagtcggc ttaatattat actctttaca
    29041 taaatacata agaacaggat agttagttga aaaaccatca tcaaaagtta gaactatgtc
    29101 tttagcgtta aatttttttg gatttgccaa aacttcactt aacgaccgaa atttatatcc
    29161 ccggcgtttt agttgtccca attgcctaaa aaaattggag acactaatac cactatgata
    29221 aatttccggg aatttatcgg ctacaatatg gtagtaaaga actcggggag tagtagctat
    29281 cttcatatat ttaatccagt tacttttaat tttttccttt taataaacaa tacataactg
    29341 tcaaggaaca ttttttaccg gaaaatccca atgccgagtt taaagaaaga aattgtgccc
    29401 tttatagcac ggaaatcctt ttattttata aatccggatg atgatttctg acttacataa
    29461 taacgactcc tcgcttttac tgacttgctt tgactgaggt ttcttatttg cattacagaa
    29521 gcaatgctta cccaatacta atgccattag caatgggtaa gcaatgggta agtaatgcta
    29581 ttaaggaagg caagaaaaaa cggtataaca gcttggaata aaaatagata taaagttaag
    29641 cccaaaatat gcaggggata aaaataggga aaaaggaaaa aatttctgta gtatattgca
    29701 atactgtatg ttgcaaataa cataaacaag gcaggttttt aatgattgag gcaaaagttc
    29761 ttgcttgaca gaaaaagaca ataacaaatt tagtgctgat taaagatcgg gatgtagcgc
    29821 agaccggtta gcgcgctcgg ttcgggaccg agaagtcgga ggttcaaatc ctctcatccc
    29881 gaccattttt tgtataaatc actataaaaa gaagcagaaa aaaggtgtaa aaagtggctg
    29941 gagacaagca gacattcaca tattatttga agctcctctt tccttttgtg aagaaggatt
    30001 tgggcttgct aatctttgga ctttttgcaa tgcttgtaac ttccggctta cgcttgctca
    30061 atccacttat tttagcacaa ataattgacc acagtattcc ggataagaat atggcgcaga
    30121 tgtttcagta tggcgcatat tttgtaatag ttgttttgct ttccggtctt ctttcctatt
    30181 tgcagatttt gttattatcg cgtttaggca taaaaatcat cactaaattc aaaggtaatg
    30241 tgttcagtca tttattgaag ttacctattt cctggttcaa taaacagccc gtaggggaac
    30301 ttattgcacg ggtggaaagt gatagtgaaa gagtaaaagt tctgttttcg gatttgtcta
    30361 ttactataat tggcaatgta ttgtttttca ttggtggttt tgtaattctc ttcattcttc
    30421 aatggcaaat tacgatatat attttgccgg caattattct ttgtggaatt ggctactggt
    30481 ttttgtttaa atatctgaat aaattctttc gtctgattcg ggaacgttat gccataatta
    30541 ccgctaaaat aacggattat gtgcaaggta ttcaaatgat tcaagcatta aatcaggaaa
    30601 caaaagcttt acaagatgtg gaaaaggcat cattagataa gaaaaaggta gaagttcata
    30661 cgcagataat tgaatacggt tctcaaagta tcttcagttt tatgatcaat gtggtcttta
    30721 tcattatagt aattctgatt tctgcaccta aaattatagc tgggacttta acggtaggaa
    30781 ttctgattgt ttttattcag tatatttatc agcttgtttg gcccctgatg caaatttcgg
    30841 aaaatgtaat gcagattcag cgttcttttg cttccttaaa aagaatattg gaattaacag
    30901 aattgccaac agaagatgat acctatacag gaacgaaaat acctgttttt gaacaggaaa
    30961 ttaagtttga aaatgtgtgg tttgcctata aagaaggtga atgggtgctg aaggatatat
    31021 catttaccat tcccaaaggc aaaaaaattg cgttagtcgg tccttccggc agtggtaaaa
    31081 caactacggt aagtttgtta tgcggttttt atcctgtgga aaaaggcaaa attttaattg
    31141 atggcgttcc tttaagcgaa atggatttta gggaatggcg gaaaaaaatt ggacttattt
    31201 tgcaggatgt gtatcttttc cctggtagca ttttggaaaa tgtgcgcgtt tacaacgacc
    31261 agattagcga agataaggtg caaaaagcaa tttctattgt ccaattggat gattttataa
    31321 aaaaactgcc tgaaggaatt aatgcagaat tggcagaacg gggacagaat gtttcccagg
    31381 gtgaaaaaca actgattagt tttgccagag ctctggcttt tgaagcggaa ataattatta
    31441 tggatgaagc aacagcttcc atagaccctc aaaccgaggc aaaaatccaa cgctcaatgg
    31501 aaaatctgct ttccggaaaa acagctttgg ttgtagctca ccgacttact tccgttttgg
    31561 atgccgatga aatcttatat ttcagtgacg gtagaataac tgccagaggt aaacataacg
    31621 agctgttaca gacctcccct gaatatcaaa agctggtgga attgcaaatg ttgagggtgc
    31681 aggatgaata aacacataaa atggattctg gaacagtata aggcacaaaa atggtttgtg
    31741 ctcataatgg tagttttaac ccttctgtct tccctcgtgt ctattgcttt tccttatgtt
    31801 ttcaaagaaa tgcttgacct gctgaaggac attttacaat ttccgcaaaa atatccggaa
    31861 ccgatgaaag cggtttatag aattattaaa attatgtttg ctatcggttt ggcacaactt
    31921 gttacaggcc tttatcccgg aattaggggc tgggtaaata tgcgttttga gcattctttg
    31981 cgggtgcttt attttaagca tattttaacc aaggacttca gtttcttcca gaaattcaga
    32041 acaggagata tagttacccg tttaacagat gacctttccg attatccaag ggttagctgg
    32101 tttttatgtt ccggtatttt tcgggctttc aattctttca gtatgattgt ttttgcgttg
    32161 gttgtgatgt tcagcatcaa tgtaaaactt acccttcttt ctatagctcc tctgccattt
    32221 atgatgctga tattctattt cacagcggac aaggtttata cgaattacaa tcgtaatcag
    32281 caggcaattt ctgctatcaa taatcaactg gaaatgagtt tttcgggaat caggattgta
    32341 aaaagctttg tgagcgaaga aaaatataac cgcttttttg atcttgcgct tgccaaaagg
    32401 ttcaaaacgg aaatgagtat ggtgaaacta aatgccattc tttctttaat ctggcaatat
    32461 attgactaca ttgctcaaat tggagttatc ctattcggtg gctatcttgc cgtgaaagga
    32521 gaaattacgg tgggcacttt tttcctgttt tacacctatc tttctatgat gatttatccg
    32581 ttaattgact tgcctcagct ctttgtttca ggcaaacagg catttgtaaa tatagaccgt
    32641 ttggaggaaa tgaagaatta tcctaccttg aatgacgaat ttaagggtca tcataaggta
    32701 aaagaattct ccacattatc ttttgaaaat gtatctttcc agtatcccga taagccctct
    32761 cccgtgctta aaaatgttag ttttgaactt ggcagcggag aaaaaatgct tattttagga
    32821 gctacaggca gtggaaaaac aactattgcc aatcttattt taggactgct gaaagccaat
    32881 agcggcatca ttaaaatcaa tggaaagccc ataaatgaaa tagatattaa atccctgcgt
    32941 aatattgttg cctatgttcc ccaggagcca cttctttttg gtggttcggt gaaagataac
    33001 atcctttttg cggtagaaca agcatctgaa aaggattacc aaattgcagt taaaacctct
    33061 cagctggaag atgaaattgc ttcttttccg gatagagata atacctttgt aggaaataga
    33121 ggacttagcg tttccggtgg tcaaaaacaa cgattaacca ttgcccgcgc cttaataaaa
    33181 aaaccgcaac tgcttatttt agatgatata actgcttcct tggatgcaga aaatgaagaa
    33241 aaactttggc aggcactacg ggaaaattat cctgatattt ccgctatcgt aatttctcat
    33301 cgtctttcta ccctgcatta tgttgacagt gtgcttttta ttgactctca gggctatgct
    33361 cataaaggtt ctcatgatta tttggttttt aataatcagg aatatcacga ctttcttaga
    33421 gagcatctaa aacaggaaaa cgaaaaatag gattgacact tataggacac ttggaaattc
    33481 tggcagtgag atttttgaga ggagaatata atgcctcgta aatgtctaat aattttgcta
    33541 tgtgcttttt tcacactatc ttatgccgat gaactggaag ataaaatgcg gcaattgaga
    33601 gaaatgcaac gccaattgga aagcacagaa caaaaagtga aacagacaca aaccaaaaaa
    33661 cagcaaaccg aaagtgagat aaaacgaact gcttctctga aacaaagaac agaaaaaaac
    33721 ctgcaaaagc tgaaaacgga agaaaaaatt gtacaggatt ctcttaaatc agttcagcaa
    33781 agaattgcgg ttacggaaat gcgtttgcag gatatgcaca gagaacaaaa tgcggaactg
    33841 gatttgcttt tgcgagtgga taaatcctat aagaaattaa atataaatca tcgtgatcag
    33901 cgtttactga aggattttat tcaaggcacc agacaaaaaa tagatattct ttccggctat
    33961 aaaattgcgc ttacacaagt tcaggaactt cataaagctc acgctacaaa ggtttctcgt
    34021 ggtatgcgcg aagaaagctc taaaaaatcc agctacgaaa aacagattaa aaccctttcc
    34081 agtcaaagtc aaaaattaac tgaagaacag aaaagattac aggcacaggt ggaaaaactg
    34141 aaaaaggaca gcgctgaact ggaaggtctc attgctcaat tgatgaaaca acaaggcaaa
    34201 gaaccaccaa gctatcaatt taccggcaaa aaaatctcct ggccactaag aggaaaaata
    34261 attcgtagtt tcggacagga aacccgaagc tatggaacaa gcgtagttag taacggaatt
    34321 gatattgcag ctaaagaagg aacaaaagta gttgctgctg atgatggaga agtaattttt
    34381 gctgatcgct acggtggaca aggcaaactt attattattg atcataaaaa cggctttttc
    34441 actctttatg cctataacag tgaattactg gtaagcagag gaactaaagt aaaaagaggt
    34501 caaaccattg ccaaaagcgg aatgaccggc tcggcaagtg aaccctcatt acattttgaa
    34561 ctgcgtaaag atggaaaagc tatcaatcct ataccgtatt tggaatgagg aggaagaaat
    34621 aatgcttgtt ttgaaagacc ccggcttacg caagctggaa aaatttattg ctaataatcc
    34681 gctaacccat tcaactattt tgcattggac tggttcaatg tggggaatcg gttgccgact
    34741 ttcctcttca gaaacaattg ctcgcataga aacactaaaa caaagagcgg ataaacaggg
    34801 ttttattgct cttattcccg atatgagtgt tttagaacag gataaaattc ctgcacctat
    34861 tagacctttg atgaatcagt attggcctgg aaatctaacc ctggttttta actatgatga
    34921 ccctcaattt gccggaataa aaaatggaaa taaagttgcc ttaagagttc cttccgatcc
    34981 tgctttaaga gctttaattt ctttgctggg agaacccctt atcagtacca gcgttaatgt
    35041 ttcaggtctg atgccggaag aaaattatga gagaattgtg cgtaattatg attcttggtt
    35101 tgattttgct ttactacctt cttcccggga aatgaaaaga ccatccgaac cttctactat
    35161 tatagaatat atcggttctg aagaatgcgg aagtaatgac gatataaaat gtatacgaga
    35221 gggttctatt cctttttatg aaataaaaca ggcgtttcaa ctaccggtag tgatgtttgt
    35281 ctgttcagcc aatatttgtc gttctccaat ggcggaaaag ctattccgca aaatgattag
    35341 agaacacaat ttaggactgg caacagattc ttgcggtttg cttcccggag gacggatgat
    35401 ttccttagaa tcacttcagt tgctgttgga acgaggcatt attgatgctg aacaacatat
    35461 ttctaaacaa gttactcccc agttgatttc ctccagttgg ttgattttaa cgatggaaga
    35521 aagacagcgt gatttattgc gcagtgccta tcccaataat gcacgcagga tttggactct
    35581 gaatgaaata gttgacgaac agggagatat agatgatcct tttggaaata atctggaaag
    35641 ctataaaaaa acctgtgaaa ttattgaaga ccgtttgcag agattaacgg aaaagctgaa
    35701 aaataatgaa ttgaggatat agaagttaat tttatgaaaa aggatgaaat aacacccgaa
    35761 cttgttgctg agcataactt aagccctgaa gaatatgaac gcattttaga tattctaaaa
    35821 cgtgaaccga cttttgtaga actgggaatt ttcagcgtaa tgtggagtga acatgcaagt
    35881 tataaaaact ccgctttatt attaaagacc cttcctaaag agggctcctt tattttagcc
    35941 aaagccggag aagaaaatgc cggcttgatt gatattggac agggcttagc aatatctttt
    36001 aaaatagaaa gccataatca tccttctgct ttggaaccct atcacggtgc tgctacaggt
    36061 gtaggaggca ttttaagaga tatttttact atgggtgcca aacccattgc tgttctggat
    36121 tctttgcgtt ttggaaagcc ggaaaatcct aaagttcagc atctattgaa agaagttgtt
    36181 agaggcatag cagattacgg aaattgtttc ggagttccca ccgttggagg agaggtctat
    36241 tttgaagata gctatgaagg caatccttta gttaatgcta tggctgtagg aatcgtgaaa
    36301 cataataaga tagcccgttc aactgcttcc ggaattggga atgcagtcct cattgtaggc
    36361 gcaaaaacag gacgggatgg aattcatgga gctacttttg catcagtgga tttgagtgaa
    36421 aaatccgaag aaaaaagaac agccgtgcag gttggagacc cttttctgga aaaactgttg
    36481 ctggaggcaa ctttagaagc aattgaggaa gggcttattg ttgctattca agatatggga
    36541 gctgcgggtt taacctgttc cagttcagaa atggcaggga aaggaaatgt aggtatggaa
    36601 attgatgttt ctcttgttcc gcaaagagaa acaggaatga ctccttatga aatcatgctt
    36661 tcggaatcgc aggagaggat gctcgtgatt gtgaaaccgg aaaatctgga acgcatcaag
    36721 tccatatttg ccaaatggga tttagatgca gttcaaattg gtaaagttac cgccgataaa
    36781 atactccggg ttttttacgg agaagaaaaa gtagcggaaa ttccagctga aacacttatt
    36841 ttaggtggct cggcaccggt ttataaacgc gcaagcaaat accctgaata ccttgaaaca
    36901 ttatataatt ttgatccgga taaagttccc gaaccctcaa attatgcaga agttcttttt
    36961 aagcttattt cttctcccaa tctttgcagt cgctttccgg tttataatca atatgattat
    37021 atggtgcaaa ttggaactgt tgtagaacca ggttcggatg ccgcagtaat tagaattttg
    37081 gatacggata tggcaattgc tttaagcacc gattgtaatg gcagatattg ttttttagac
    37141 ccctattcgg gaacagtttc tgccgttgca gaatccgcca gaaatgttgc ttgcagtgga
    37201 gctaaaccaa ttgccatcac caattgtctt aattttggca atccttataa gccggaggtc
    37261 tattattgct tcagtgagac aattcggggg atgagcgatg cctgtaaaat tttaggaact
    37321 ccagttaccg gaggtaatgt atctttctat aatgaaaatc cggaaggagc aatttatcct
    37381 acaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctta ataaatagcg
    37501 ctcatgatgt ttccgatggt ggattattag tagctgtatt ggagtgctgt tttgctccgg
    37561 ataatatttt tggagctaca ctgaattttg ctttttcgga taagccctct aaggtctatt
    37621 ttggtgaagc aggcggaaga attttaatta gtgcgcttcc ggaaaactgc caagctatta
    37681 aagaaactgc cgaaaaacat aacatagaat atcacgaact gggttatgtt tcaggaaagg
    37741 caaaatttgt tgtaaataac catatagaag aagatataga aattctttac tctggctgga
    37801 agcagggaat aaaaatataa aataaaaagg ataaggaaat gaaaatggat tttatttttg
    37861 gcaatatctt ttggggttta ctgataatcc ttttgggatt atccattttt ttgaaaggat
    37921 ttaaccttca tattcctttg gtgagagtgt ttattgccat tattatcatt atgtttggca
    37981 ttaaactgct cttcggtatt aaaacgcata aatataataa gccaaaacat tattccaggg
    38041 aaagcactat taactatagc ggttctcgca acgaatataa tatcatcttt tcctcgggca
    38101 taattgacct cactgatctt cctgccgatg caaaagatgt agagattaat gtggttttcg
    38161 gttcgggaac agttatccta ccggatagca ttgttttcaa tattaatcct acctctgttt
    38221 ttggctcaac tgttcttccg cccaaaagac ctgaactcca gggaaaacct attgatatag
    38281 agtcaaatgt ggtttttgga cgcttggaat ataaatatgc accaccccca aaagcaacag
    38341 ctaaacacgc cgagacggaa aaagaaacat tttaatgcct aaccgatatg gttttcattg
    38401 aacaaatgca gtttttatgt tcattcttag gtgtatttat ggattcctac ttgcgcaggc
    38461 aggacaaagg aatatgtaac ttttaattgt tatgaacact gtgaagaaat cacttatcct
    38521 actgttattc atcttctttt tgaatagctt aactgccgaa aaacttggct atgccttaag
    38581 tggaggtgga gcaagaggtt ttgcccatat aggtgtttta aaggttttgg aagaagaagg
    38641 cattcatcca gattatatag ctggaaccag tattggagct attattggag cactttatgc
    38701 gatgggttat tctgcctccg aattggaaga aatgtgtctg aaactgaatt ggaatgaatt
    38761 aacgcgggat acacatcaaa gaaaagacct ttatatcgga caaaaacgct gggcacctta
    38821 tggaaatgct gtttttgaac ttaatgaatc ctgggttcca caacttcctt cttccgtttt
    38881 tgtaggtaat aatcttaatc tggaattatt taaattaaca gcacccgctt ctcagattca
    38941 gaattttaat gaactgccca ttccttttat ctgtaatgca accgatttat taaccggcga
    39001 agctgtttct tttacagaag ggtcgttact tcaagcattg cgtgcttcta tatccattcc
    39061 aagtttgttt gagccctttg aactgaatgg tcactattac attgatggcg gtgtttccca
    39121 aaatatgccg attggccttt tacaccaaat gggagcggat aaggttctgg gtataaaagt
    39181 taattctacc ttgcgcgatg aggaaagaat taataacctg gtagaaatat tagaccagac
    39241 cattaatatc ggtataactc gcaatctgaa agaacatttg gatgaatgtg acctgctgat
    39301 tgaaccggaa cttacgcaat ttgtttctac cgattatcaa catttgcagg atattattgc
    39361 tgccggagag gaatctgcca gacaaatgat tgatcaaata agggctttta agaatgaatt
    39421 ttattcctcc agtccaaaaa taactacctt tgacaagaag ctaaatagtt tttatataac
    39481 tcaaatcaat gttcagggaa ataaaaatat cagtccttcc aaagtaagag aatatgtggg
    39541 tttggaaaca gggaagacct atagcagtga agaaattgct aatgcttgta ctaatgcctg
    39601 gaattcacaa ctttttaaga ccatctatcc ggttctccaa aaaaatcaag acaatcaata
    39661 tgatctctat atctatgtga aagaacggga acgtaaacaa ctggctgtaa acttagttta
    39721 caatagcgaa gaaaagctga tggcaggact tgttctttct ctaaataact atctgctcaa
    39781 aaattcccgc cttttggcag aattaaaact gggtggaaga aatgagttaa atgttgatta
    39841 tgtgaaaaac ttcggtgaag agtggggtgc ctattatcgt cttttccctt atgtatgcga
    39901 aagaactatc tatgaatata gcgaacacca taaaatcaat agctttaaat ctacggaatt
    39961 cggtatcaat tcgggagtgg gagtttttgc taaagacctt gctattgcgg aattttttgt
    40021 ttatacggat caaacaaatc ttcacagcga tatttctcaa accccattat tacctgaaag
    40081 ttcggtagtt agcggttttg gtataaaggg ttatcacgaa agtttggatg attttatttt
    40141 tccgacttcc ggagccaggg ttttcggaaa gttcaatttt gctcgctata gtgaaattag
    40201 tgattttatt tatagctgtt ttcaaggtag ggcagagttt tatgttcctg ttagaaatat
    40261 tttcagtgtc cattcctcaa tctattatgg cagttattta aattctacgg aacacgatat
    40321 acttgatcct tttataatca gtggtacaga tggttttatg ggatattcca gatatgaagt
    40381 tagtgctccc cactatcaaa ttttatcatt tgggttttct tcattgccta aaaaccgcta
    40441 ttttattgat gccggatttc aaggtatgcg ctattccgaa aaggatgttt ggagtaattc
    40501 tgaagattgg gaatattgcg gatatttagg attgggctac aaaaattcta ttttacctac
    40561 caaactatat gtttctttga ataaggataa gaaagtgcat agtttcttaa gtatcggcta
    40621 tgattttgat atctttttct tctcccgcag gtagggtttt agctctcaca aataacacag
    40681 ataataaaga taacacagat tttttcattt aagataaatg gctgataaac cctttgcctt
    40741 ttgtctattt tttctacgca ctattccttg ccaagttgaa tcttttatgc ttctttaaaa
    40801 cttctttaac gattccttag cgaagttagg gtattgttac aggatcgtta aagtgtcgtt
    40861 aaagaaatga ctatgcaaca ggtagttaga cgaagtcctc aaggtaattt ttataaatca
    40921 taattatgac gggagcaccg cttctgtttt gtaatttcat taaagataaa gctgcggtta
    40981 atataatctt acgaactacg agaccatcgg gtaaggtatc tattgacagc ttaattttgt
    41041 ttactatctg gcaatcaaga cattattttc gtctttctat gaaatttatc ctgattttta
    41101 ttatcacaga ttgtctcaag ccgcttacat atttctttcg ttattatccg tatctttgaa
    41161 ggtatttatt tctccaagca ttgttttcac tatctcacag agttcacgtg atttttccgg
    41221 agaatcgctt tccacataaa tacggataat gggttcggtt cctgatttgc gaatatggat
    41281 gaagtgtttc tctccaattg ctctaattcc atcccgagta tccagttgat agttttgaaa
    41341 gaaaaaagga gcatcattca tcaatgtctc catcaatgag gcatccacca ttattttatc
    41401 cttggcaaaa taatactttg gcaagctatt aactatagcg gaaagcggtt tatttgtctc
    41461 cgcaagcaaa cctaaaatca gagccattcc agcaatggca tcacgggtgt aatgtacatc
    41521 aggacagata attccgccat ttccctcacc cccgatagga gaatttattt cctgcatttt
    41581 ttttccgaca tttatttcgc cgacttttgt ccgctgaact tgcacgccaa agcttttggc
    41641 aatatcatcc gaaagcattg aactactaag atttactaca atatctccct tctttttggg
    41701 aagcacaaaa agctcggcta aggcaacgct caattcttct ccgatgcatt ttccttcttc
    41761 ggaaacaatt gccaggcggt caacatcggg gtctgtagca aaacctatat ccgctttaaa
    41821 aaagctaaca gtttcttcca actgacctaa attttccttc aagggttctg ctatgtgggc
    41881 aaaaatgccg ttgggctcgc tattgatttc ataaacagtg cagccaagtt cttttagtaa
    41941 aagtggggaa atcaaaccac cagcgccgtt tacggaatcc agaaccactt taaattgacg
    42001 ggaacggatt tgttcaatgt tcaaataggg aatgtttaaa attttggcta tatgttcttg
    42061 tatggcagta ttatctattt ttaattctcc cattgctttc cactttgccc agggaatatc
    42121 ttcttctacc gaggacagga atttatccgc tttttcggga cttaagaaca ttccatctgc
    42181 atctacaaat ttcattgcat tccactccgg tggattatgt gatgcagtga tggctatgcc
    42241 tccaatggca tccgaattct gaactttcaa taggagagta ggagtggaaa caatgcccaa
    42301 atccgttaca tcacaaccta cacttattag ggcagaaatt atactgtgca acattgcttt
    42361 acctgttgtg cgactatctc ttcctacaat aatctccggt ttccgttcag gatagctttg
    42421 tttctgcaga attgcaaaat gagctgcata ttttattgct ataataggat taagggtgtc
    42481 tgcaaagaca cccctaacac cactaatacc ggtcattaat ttgttcattt atataatacc
    42541 gtattttgcg cggttatcaa ttattttagc cgacttgcgc atttggtcat accaacggtt
    42601 ccaggcagcg tcttccaatg ttttacgaat ttgttcctgt ctatccttat ctttggcgaa
    42661 ggcattgaaa tcgggcttaa ttctttccgt gcaactgatt atgaaacttc cttctttggt
    42721 atgaataagt ccgctggttt gtcctgcttc caaagctaaa gctgcattgg taaattcctc
    42781 gcaaagccct acattaggaa cgctttgatc tcttttatga ttgtttaatt caattatttg
    42841 ccagccctcc ttggcagcgg cggagaaata ttcactggaa ggatatttgg caacgaattc
    42901 ttctgctttt ttctttacat tggctacttt cttttctttt tccagctgat atttaattcg
    42961 tagcttaact ttttcaaaat cctcataata gacctttttg ttatctgtta acacggcaac
    43021 gattttgcgg ttttgttgat cggtaattac atcagatatt gctttttctt tacctttacg
    43081 cataaattgt aacaaagctc cgttattgcc aataccggga atataatcct gatcatgggg
    43141 cacccaatct gtttcctgaa cttcaactcc caattctttg gcggcagcat caattccttt
    43201 tttcttaatt aacttggcag cagattcagc tttatccagg atagcatttt tggtttcatc
    43261 ggatgcttca actttcaata gtatatgact tgctttgatt tgaggattat ctttggtaat
    43321 tgtagcaatg ctgtcacaac ggataatatg ccaaccgaaa cttgttttaa cgggcttgct
    43381 gatttctcca acctttaaag caaacgctac ctgttcaaat tcaggaacca tttgtccttt
    43441 accaaaaacg cctaaagaac cgccattatc tttagaacca ggatcatccg aatattcatt
    43501 agccagagta gcaaaatcct cacctttgag ggcacggtta taaagttcat cagctaaaag
    43561 tttaactgag gcataatcct tatctgaggg cttttcttca aaggcaagat atttaatgcg
    43621 ggaagcggga ccttttttat attcggtctc tctattttta tcataatatg cctttatttc
    43681 agcatcagta acggttacag ggtcggattt attgggttca aaccagataa attttccgga
    43741 aacgctatcg gtttctttgg catattgcgc ttttaagctg tctatagtga ttcctgcttg
    43801 ggctttaatt ttttcctgca gtttttgacg gggcagatat ccccgaacat attcttccat
    43861 tgcggcaaag aattgaggat tgtttttcag agcagaaaga tatttggatt tatcaaatct
    43921 accgttggtt tgcagggatt cgttttgcat caattcttgt ggcgggttat tttgcatttc
    43981 ggttaagaca tcatcttccg taattttgat tttgtgtttc ttaatctgct gctgccaaag
    44041 aatatcgttt accaatgttt cccatgcctg attttcaatg tttctgcgca tattttcatc
    44101 tatgggctga tctttataca tttcagcata gcgttcatac atttcttgta tcttttgctg
    44161 atacatctcg taggttattt tggtgccatt cacttttccc agatatggtt tcggggataa
    44221 taattctcca attccaacag cagccatacc cagaatgaat acgatagcta caatataaat
    44281 aactaccttc tgtttctttc tcagctgatc cagcattcta aaactcctta tagaggttaa
    44341 attactaata taaaagccat ttttttgaag cttcattttt taacaaggat ttttttcata
    44401 cttgttttct gaacgacagg gatgctgttc atcctgaagg ttgatgtcct catcaaactg
    44461 aatatgtgca ttggggtaat aaaaggcaaa cattttgctt gacgaataag agctaatcca
    44521 agttttgggt ctgaaagtaa aactgttaga gaatataata atgtaaaagg gagcataagc
    44581 caatgaaaaa gttttcctgg cgtggcctag tcataatcat atttttctgt gtaacagcat
    44641 tttttttggc tcctatggta attcccaatt tgccaagttg gtggtctaaa catcatctaa
    44701 agcttggttt ggacctaaga ggtggaatgc agatactttt ggaagttgat acttccgagc
    44761 tttcagctgc agatgcccga aatgctgtag aacagaatat taaaattatt cgtgaacgaa
    44821 ttgaccagtt tggcgtagct gagccctcaa ttcaaaaatt aggtgaaaac aggataatgg
    44881 tacaattacc cggtgtttcg gattataaag cggctgaaaa tttaattaaa cggacagcta
    44941 tgctggagtt taagctggtt gcttctcaag atgaagctaa acgggtaata gaaacaattg
    45001 atagaaatat cagcagtaat ctggataaat ttcccatttt agcagaaatg gcaaagaaag
    45061 ataaaagtat aatagctgat accacatcta cagatagttt gaaaaccgga gaagggattt
    45121 tcagttctct gattcgtcct ggagaaatgg attttgaggt tcaacacagc gatgtgcggt
    45181 taattcagga tttgcttgcg gatactcttt ttgcacaaat ggtgccaatg ggttatcagc
    45241 ttgctttaga taaggcagat acggaaaatc cccgtgccga tagaaccctc tatgttcttt
    45301 cttctgcagc tcagcttact ggtggtgact tggctaaagc aaaagttgaa tatggttctg
    45361 ccacttcaac cgatcccagg atagccaata agccctatat ttccatagaa atgaaacgag
    45421 agggggcaag aaagtttgaa cgcgtaactg ccgataatgt aggaaagcgt ttagctattg
    45481 ttcttgatgg agttgtttat tctgcaccga atattcagga aagaattgcc ggtggtagag
    45541 ctcaaattac aggtagattt actgctacgg aagcaaatga actagcgatt gtgctcaata
    45601 caggaaattt gattgctcct ataaaaccgg tttcaacttc cataattggt gctactttag
    45661 gtgcagatag cattcgtaag ggaactatgg caggtttaat cggtttaata cttgttgtcc
    45721 tatttatggc tatctattac aagttgtgcg gttttattgc cgatattgca ttggtcttaa
    45781 atgtaggttt tattttagca atgttaacgg cttttggtgc tactcttact cttcctggaa
    45841 ttgccggtat tattttgaca atcggtatgg ctgttgatgc taatgtgctt atttttgaac
    45901 gcataagaga agaattggaa ttgggaaaaa ccccccgcag tgcagttgaa gccggttaca
    45961 aaagagctct tgtaacggtt tgggattcta atctgactac tgttattgcc ggaataatcc
    46021 tttattatct tggcacaggt cctatccgcg gttttgctgt aactttagtt attggtatta
    46081 tgggttctat gtttacagca atagttgtgg tacgaactat tttggataat atggttttaa
    46141 ccggaaaaag aacaaaactg agtgtgtaag gagtaacaaa atgagaattt tgaagaatac
    46201 taatatccaa ttcgtgaaga gccgttactg gttttatggt atctccattg ctttgttact
    46261 aatcagctta tgcggaataa tttttaaggg tttgaactgg agtattgact ttaccagtgg
    46321 aatagctgcc accgttaatt tgaaagcatt agaccctaaa gttcctgctg ttcctattga
    46381 gaatttacgc acagttctta ctaaaaacgg ttttccggaa gcagagataa cctatattgg
    46441 caatccagct aattccactt tccagattaa aataaaaagc gttggcaaag gaactgatct
    46501 ttctaccgaa acccgcacta agttattgga aatcattcag caaaattttc ctgaatatgt
    46561 tcaaggaaga gacctgaata aagaagtgat tgaagaaatt tatgaagttg gtcctaaagt
    46621 tggtagtgaa ttacgctctc aggcattttg ggcagttatg cttgctttaa tagcgatgat
    46681 tatttatatt tggttccgct ttgaatttac ctttggctta atgggaatcc tggctctttt
    46741 tcatgatgtc tttattattg taggtatttt cgctcttacc ggaagagaaa tgacaatgca
    46801 aattatagct gcactcttaa ctattgttgg ttatagcatt aatgatacta ttgtgatctt
    46861 tgatcgtatc cgtgaagata tgaagaaaaa ccgcaaagac cctatccagg tggtattcaa
    46921 tcgctctctc aacaagaccc tatcccgaac tataattacc ggtggaacta cttttctaac
    46981 ttctgcttgt ttgtttttat ggggtggaag tgtaattcac gattttgctt tggctatctg
    47041 cctgggtatc atttttggaa cctattcttc tatctttgtt gccagtaatt tggtgattga
    47101 cctgaatttg attacccaca aagaaagaaa aacaatgcaa cacctaacaa agaaaaaatt
    47161 gtaataatat cgtatttact aacctatata attagaaccg ctcataaatt gtgggcggtt
    47221 cttttttttt acacagagaa cactgaaaac actgaggaca ccgagaattt attttacaaa
    47281 gagtaaaaga gaaaagagat tgtttggaaa aagagaaaag agatttagtt ttacaacaga
    47341 aaaataaagt gtagccggag gacgccgttc ctccacaagt aagtgaaaag gtggaacagt
    47401 ggaaaggtga aacaaggttt ggacattgta aaaaaaagat atggagcttc agcgagctcc
    47461 ggctacacaa attaggagtc atcattgctg aaataaatta taacctgctc tcaagcttat
    47521 ttagtgatga ggactcgtga cgatttttaa ccacagagaa cactgaaaac actgaggaca
    47581 ccgagaaatt tttttacaaa gagcaaaaga gaaaaagagc aaaagagaat gtttggacaa
    47641 agagaaaaga gcaaaagaga aaaagagcaa aagagaatgt ttggacaagg ggaaaagaaa
    47701 aataagagaa tgtataagta aagacatctc acattcttta tttcctcaag acctcttaac
    47761 ctcaagccct cttaacctca agacctcttg attttttgac ttcaagacct ccagacatct
    47821 tgacttcaag acctcgtgac ttcaagacct c
//
LOCUS       DAOOZV010000009        43422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1009_length_43422_cov_69.969631, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000009 DAOOZV010000000
VERSION     DAOOZV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 43422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43422
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_43422_cov_69.969631"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(628..915)
                     /locus_tag="PLW08_01950"
     CDS             complement(628..915)
                     /locus_tag="PLW08_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL51397.1"
                     /translation="MTKADLVKIISENTGIIRKDVAVVVDALLYAIKDSMAKGNHIEI
                     RGFGTFKLKTRKPRVGRNPKTDQKVPVPARTVPTFKFSREFKNAVVNVKTK"
     gene            1370..2005
                     /locus_tag="PLW08_01955"
     CDS             1370..2005
                     /locus_tag="PLW08_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51398.1"
                     /translation="MQTAVQDSVPDVYSFRCEGKKIILQHSEKKYILYYSKGLNYILT
                     LLEYPELNIPYPNLESNLSSPEKAYSQFSSPQEIYEQQLKVQDGFLAVPMTDLQTIKE
                     VKEELIQVINELAELEENCDYARADELRDIYEKLSKYLRDVYHKDCKIRHFTSEESKI
                     KRRVVRAVNRALEEIEIIEPELAKELRKSIEFGKTICYHQRRKIEVSGYWK"
     gene            2360..3502
                     /locus_tag="PLW08_01960"
     CDS             2360..3502
                     /locus_tag="PLW08_01960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL51399.1"
                     /translation="MDAFSFCNPTRIEFGVGTIRKLGMELKKADIKSCLMVAGGGSIK
                     KNGVYEQVCESLEKAEIHFVEGWGVQPNPTLEKVIELCELAKREKVEAVLGVGGGSVI
                     DTAKTVSAGVFLQDIWNAFSGKEKIRNALPVYTVLTLSATGSEMNGNAVITNTKTMQK
                     WGIYSPLIYPRLSIIDPSVQSSLPFKQTANGAMDAMAHILEYYFADDRAIATLAIDDA
                     LLKTIVEMTDRLQNNSGDLLARGNLAWCATLALNGISGIGLKGGDWACHDIQHAFSAL
                     HPEIAHGEGLGVIFPAWIEYMSEKDPTRFLRWAKNVWDEENVSRALHRFRDKLESWGL
                     AKSLRDLGIKELELPQLVKLIMTSPRIGMVSRFTAVEVESLLMLAF"
     gene            3734..6895
                     /gene="secA"
                     /locus_tag="PLW08_01965"
     CDS             3734..6895
                     /gene="secA"
                     /locus_tag="PLW08_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPL51400.1"
                     /translation="MLEKILKKVFGDKNSQDLKRYEPVVQEINEIFSGLEQYEDEQLI
                     ARVQEIKQEIADKLTPLRNELAKLEQEYRETREDSERNRLDNAIDSTKKELKNLTKST
                     LDDYLPEVYAIVKDTCRRLLGKKFEVRGHEVEWNMVPFDVQLIGAMALHDGKIAEMAT
                     GEGKTLVATMPLFLNALVGRGVHLVTVNDYLASRDAEWMSPVFQFHGLTVGCITTGMD
                     FDARKEAYNCDITYGMNSEFGFDYLRDNMATSPDQLVQRDFFYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYHELRPAIAQLVQLQNSLVQKYLSEIREDLDENNPQSDNNRLA
                     KNLLLVKRAAPRNKAFMKLMQDGELKKMVNDIEGIYLRDKKLPELDNNLFYVVEERHN
                     SVDLCEKGREVLSRKDKDLFVVQSLDEMLAEIDNNPELSEQEKQKEKSLQTNRFMDKS
                     EKLHNINQLLRAFTLFENDQEYVVIDNKVVIVDEFTGRQMPGRRFSDGLHQALEAKEN
                     VEIEAGTQTFATITLQNYFRMYEKLAGMTGTAVTEEAEFMEIYNLPVMVIPTNVPVTR
                     IDHDDLIYLGKNDKYQAIINEITYWHERQKPVLVGTVSVEVSEIISRLLKRKGIAHNV
                     LNARQHQREAEIIAEAGQPGAVTIATNMAGRGTDIKLGKGVVEGTYESYLNLPKTLTA
                     EFPYGLPLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLMRLFGSD
                     RIAPMMVKMGLKSGDVIRHPWMTKAVEQAQKRVEEHNFEIRRELLKYDEVMNQQREVI
                     YAYRRSVLKGYDLKNEILEMITESITNMVDDIIPPHSYPEDWNLERICQWFQHNLNLG
                     LTVTDIASDHLNRDLLLNTLLEYALSAYENKEKQIGSEQLRNIERRALLEVVDDEWRD
                     HLHEMDLLKDGVYLRAYANKDPLIEYKKESFELFQGLITRIQENVTRKVFTTYVLSQE
                     QINNLLKNANLTHQDINAFLNAQQAQQMTQNMSAPPQYNNPVGEIEKVRPIKVAPKVG
                     RNDPCPCGSGKKYKKCCGINEAE"
     gene            6941..9160
                     /gene="topA"
                     /locus_tag="PLW08_01970"
     CDS             6941..9160
                     /gene="topA"
                     /locus_tag="PLW08_01970"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPL51401.1"
                     /translation="MSKGLIIVESPAKAGTISKFLKNQFSVKASMGHIRDLPKHELGV
                     NVHQGFKPVYIIDKTKSKVIAELKEATLSADSVYLASDNDREGEAIAWHLSETLKKEL
                     NNKPVYRIVFNEITSHAINEAIKNPGQIDMAKVEAQQARRILDRLVGYEISPLLWKVI
                     TKDLSAGRVQSVALRLICEREAEIKAFVPKEYWKIEADFWKNNLPPFKAVLEKIEGKK
                     VEIPDEKSAIEIVENIQNAEAILSEIKRSNRNVEPLPPFITSTLQQEASKILGFQAQK
                     TMSIAQVLYEGIDLGGERTGLITYMRTDSTRMAEEAITKAKDLIKERFGKELVHPKVR
                     VFKNKQSAQDAHEAIRPTDPSRTPESIAQYLTKEQMKLYTLIWQRFIATQMIPVKLLS
                     TSVKINAGKAEFVSSGAQIMEEGFLKAYPHYNIPLGEKIHLDYAKNDVLEHSPLEKTQ
                     HFTTPPARYTEASLIKELEAKGIGRPSTYATIIETLRKRKYVTMEKKAFLPTQLGSDV
                     NRFLVDKFDYLFNVQFTAEMETKLDEVEYSKIAWNEVVQEYYDQLIALIRQVDVKKEK
                     NNFVQDTGIVCDVCKQGTMLIKHSKRGDFLSCSRFPQCKNTKNFTHDEDGNIKIFVPT
                     PLNENCPQCGSPLMLRNGKYGEFIACSNYPKCKYARPKTLGIKCPECGIGELTARKSK
                     KGRSFYSCNRYPECKYITNDKPLPISCPKCGNPYIVEKYNREKGKIKICPKCKTEME"
     gene            9164..10435
                     /locus_tag="PLW08_01975"
     CDS             9164..10435
                     /locus_tag="PLW08_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51402.1"
                     /translation="MNIKDGINSAFQSIFSHKLRSLLTLTGIVIGVLAVVTMFSSVYA
                     IKALIKKNMEGMGWDNSIIIFPGSGNIDLETGKTRSKIRRAKQNVPPLNYDDYLALKE
                     NLNYKCIYGTISQHSLYRVGNKINNIILRATEVEFFQNKSYPISKGRYFNAYEAENNI
                     PVAVLGYHFAEEYFKDKDPIGQVLVLGSQRFTIVGVLASDVLNTGNGMNFDPWQREWD
                     LKAVYIPLKYGATYLSPARMIHQIYIQSNSSEDYAKLKNEARQILLARHNMYPNFQFM
                     DIGDMILNITQEINKFMDKWNVTLIAIASISLIVGGIGLFSTLLISIQERMTEIGIRK
                     SIGATEQDIFFYFIFEALALAFIGAILGVVLAWILIVLIAKGINFPLYLPVQGVAVGI
                     GFSLLVGFLSGIYPAWKATGIDPIQAIYYHE"
     gene            10525..11106
                     /locus_tag="PLW08_01980"
     CDS             10525..11106
                     /locus_tag="PLW08_01980"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPL51403.1"
                     /translation="MKKNDKIAEFLAKKRLLFTRILCIAVTLEFVINYKVRIQHLLTN
                     PSGIIISIILIALGAFIRSWSAGIIQKGNRLVQEGPYCLNRNPLYTGSALILIGFVFF
                     LNDIWAWIVAILLILVIFPFTISREEKGLRNKFPEEWINYSKTTGRYFPRKISWKKMK
                     YPWSFQLWLKNREYQTFFLSLLLLIIIIIVGLV"
     gene            complement(12241..13668)
                     /locus_tag="PLW08_01985"
     CDS             complement(12241..13668)
                     /locus_tag="PLW08_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51404.1"
                     /translation="MSKLLNLTLHYKGKKLDQVRYGRDFKNKFFIGSDKYLFWQILDE
                     KFPEKHLFVTKKGDQLYLQLPPGAKVSCAKDGEPVDESFLTKSFILQGNKLLLRPDMT
                     GTLAIAPNWEISYEFKEPYVSVLTPEQQQIAAQYACRPQPTAEEKFSRNMILLFVILT
                     VIFVIIFDLFLKKQVSYDTTLEQKLQTIQKAERIQAEALAKPSTFEEAGEYAEVPEET
                     AEKGQPGGVTGGKGTAKSAGAIFGSGFGTFDPSATSAARPVQALTLMEGFVTSRPGGG
                     GGGGAGPGVGGAGPGGGVGGTTFDPNATRSFTSDIGSVATKAPTVRGSEIRPEGVAIV
                     KASGDQSKLMPSGVAFGKTAVTEQTLAKFKSKQVTQVTEETISQAPAERQTIIDEVRR
                     AVEIRKGQIQSLYMKWNATIPFSGSVKIRLLINASGKVQDAIITPEGNMPNGFLTELE
                     QLCESWSFPINEEFDYEFKFRLRKG"
     gene            complement(13681..14226)
                     /locus_tag="PLW08_01990"
     CDS             complement(13681..14226)
                     /locus_tag="PLW08_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPL51405.1"
                     /translation="MQILDKLKSSNKSERRVRKKFEEAKGLSITSLMDVLTIILVFLV
                     KNISTEVINISAEPNITYPHTITNDKLLEKAGTTPIKIFPDRVVVGTEALEYGSPADL
                     LTNAQKRNDLLTYLKMEAEDIIQHQKAETCLLVQADYSIPCEYITEIVRAATAAGYSY
                     IYFATLEDADWLKKYSVSLAE"
     gene            complement(14238..14762)
                     /locus_tag="PLW08_01995"
     CDS             complement(14238..14762)
                     /locus_tag="PLW08_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPL51406.1"
                     /translation="MRQYRPSAMRQQKSQTVPQEPNLVPIMNLFLTIIPMLLMMVVIS
                     QIALLALNFSQGESGVSYGQGGGGGGAKTEKVEIHILGEQYAQENNTTLGMEIREPGF
                     KPMTISAINGYYDYVTLNKTLQEIRSRKGTLSDITVLVHPDVLYGDLMKTIDICKVNG
                     FTQVHYTATKVEYY"
     gene            complement(14768..15535)
                     /locus_tag="PLW08_02000"
     CDS             complement(14768..15535)
                     /locus_tag="PLW08_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPL51407.1"
                     /translation="MKTKNIVLMIISLFMFVGLFAQPATETAAPAAPAKQGYSLIQVF
                     KDSGNWAYAILLVLVIGLVYAFVRWYQLYPKEKIDAAGFYLKLRNYIRNDQLDEATKI
                     AESYKGTTMGFIFWSALMVFKDSRKSGLTGSDLRNSVQNSLEEAVLQTVYKLDSGLFW
                     FDTLAQVATYLGLLGTIWGLLGAFAGLAGLTGAAQQTALTDGIKKAIGTTALGLMTAI
                     PLTLIKGWLLTRANKIISNIDEFSVKLINTINNAIKD"
     gene            complement(15532..15831)
                     /locus_tag="PLW08_02005"
     CDS             complement(15532..15831)
                     /locus_tag="PLW08_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51408.1"
                     /translation="MKKVFILLAVLLFFTSIIYAQAATTTTQQQPVKGFEGVRRYVEE
                     KELVIEIKALIPSTDIFQDKSDTYRKAGEYKFSFTIEDIHKQSDTLLNKWLKESK"
     gene            complement(15828..21677)
                     /locus_tag="PLW08_02010"
     CDS             complement(15828..21677)
                     /locus_tag="PLW08_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51409.1"
                     /translation="MKHNLKIIFLIVVAVSFLAVVYAQDRSQYTKEQIQEQISQEKQL
                     IRELAKMFLVDALPELVKEDPTLAEQALVKYKDVFSSMDQNEFIYLLGHFYVRVGENA
                     KAISHFTSLLNTHLNEDARKMLNLVLFQQMVHYLEQGNRKTAKDFLSAIVFENYNIDR
                     YYPTYLYIWADMAADDGEYESVINTLNSYIENRDIIMNRLLPEKMAILARVQNLNLEP
                     YYANPSPAEYKRLASQIDGIKADLTRNYNELISLKGIFYLDSIVNLHSEEMAMLDSLK
                     MVILDYIDQKVQAEETMARGNLLFQQVKQLSSNYQKQLEIMDKILEKQYERFLTNDPS
                     VQGQNFSDMELKRLFDIEKNIQLYNNLIAELDKDIADPDLANIQEQLKRTRADYSEKR
                     TSLLIRKNDLLQTRKHISDVEEQIFNALLQEYYSLSQDKKDIDVQLAEIEQFMQEESK
                     NIFTEEQRNKIKNRIATQIAVTADSGVRNEPIRKNAQDLVANVEFVKLQLDYRNLHIK
                     EQARIAQIENWTEQQLTERQAEILAEKRDLITRIQNFINNNPNFKAVEQPNGTFLISN
                     ADLYYNLAELQYAVYLETPTIALESYRKVVQLDPNNVNIDAALYNIGFISSQLSHQRI
                     GDNKARFYEINRTAVALDDASRYKESDFAEAISSYQRIIDNYKTSPYYDESLYRLGIL
                     YYYIATDADQPQRYYALATNCFDEIINKPDSKYKYDAIYQRGWLRLNSFQEQDFMLAL
                     NDFLTLLNAVDSGKISDPMIAKGYREDAIDNIAFCLIALDGSDFYSPAKGVAELQNIA
                     ASYKNPEIISSIIDKAAANKLNLSTIQAIDFIWLKIDINPLALENPTLVDSILQLYAS
                     AQYELREGQNFDQIAQDLYQNIITNYGKNTAWYQANMNNSNISAQLAVIKNAYEKRAI
                     RLQNEFLADPANETKLLAYYQHIDQYAAFPELQGENYVAWKAQADKTKLLLGNTLTER
                     SNLPKHYLTAIRNLQEFNAKYPQDEDFFLNEGLIYTYSFNIYNLMKDLYGEGFQPEPG
                     LPANADELFALLQSNSQRFISVLRSDAYKNPEREIQAVNILLSLADIQYDRGKLPDAK
                     ELYLSALENETLIPDRTKFTVYGKLASIAENEQNYAQAETYYRKALPYAATPDEREAI
                     INNICYQIQNSFEAAESAGNYSLAASERLRLAEELGSKDAQRVTGYKMGAQEAYIKAK
                     EYQKAIDILLELAGTRTDIGEIYYYYFRAAEIAEADTAMNNPQLAKSIRESFIAKYPS
                     SNQAFKLRMAMIQEMEKNPAQKNAAADAYLALYEEARNKTINTGNITPDALLAKAAIL
                     YSEAENKDKAVETYNRFITLYPNSENVIPFMQESADYYLAKGDTLKFEQLAKEIYQKD
                     KTKGDRYQWIATLKLSKLVYNFDNAYKNKNYTDAFKFRDEYKRLEAVYKKEGLNFETK
                     DFSSTKNYEYFAAVEKEYNDLQKRIAFLKNFDNQLTAIEKGTLMTSSPAALIKVNAST
                     TWQNHLIGGSYRRIPNFKASVKAEVAKVNKLLADADKQNIELENYRRLRALNLIARIY
                     EKGVNVINTQIGVYVRTASEVEGVRQEYKDVLDAFIATLAADYSNDLFSLAYTTHLNI
                     YNFYQLAGYTDYYTQQSYAKLQEWNLLPDYKMEEYPLGSGWTEKMDGTNVNLPVQNIT
                     SPKGVRLSCVQIPSNKELILNRTVNAKIQPEFALLHLVYPYDIKIQLNGTDLKPGVVP
                     TDTLDVNKPITTTRLAYLLPSSAWVEGQNIIELTVPNDSPEQQSLYMNLQVFIDRNKL
                     AASIPPETVMLYTDPTWRIITVNPETGVETASAATITGNFGITLDNIVGLEETFAKPI
                     WFPETAPVSSAIFEVDFYLDTEFRDGLINFVAPEVASVYLNGQELAANLHLDYDPEPF
                     RVYTSQVTIDPAKVTAGKNTLRFVVTNNSNYRGFIATVKIIKAGKGDIR"
     gene            complement(21831..23030)
                     /locus_tag="PLW08_02015"
     CDS             complement(21831..23030)
                     /locus_tag="PLW08_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51410.1"
                     /translation="MKKTLLTVIALVAVLGVAQAAPFQTLGMLRTPDAYVLPNKAAEF
                     LVVGYYRDISAPKPLTEDAKDASKGFFPYTMIGVGILDRVELSMFLGDYTSLDGLVYL
                     LNAKVKIIEETLLIPQIAVGMDNILSPVEKHGAQYLDVNNDFADNPEADSYEPYSPYA
                     VASKQVVFWGIPWMFNLGIGSNRFVGQAPRARYLTGTFFSVEMSPVRDLTLQGEFDGE
                     DINIGINYAYKNFGFKLGASAIEDRFKDSDYKNNIRVALGISYLFDKYAEAKRRPDLG
                     LYASYGKKPSAGGEVVEVGETPIETGVVVPPTGTTVSPTGEIVVVPPTGTAAKPPAGG
                     EVVVGPGTQLQTPGLVTPGTAGYQQLSPEVQDLLKELQMLKEERQKAQQAMEELRKWI
                     EELKKKQ"
     gene            23116..23502
                     /locus_tag="PLW08_02020"
     CDS             23116..23502
                     /locus_tag="PLW08_02020"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPL51411.1"
                     /translation="MKKYSLQDFSHIGEDLAARYLVSNGYTITCRNYRKKYGEIDIIV
                     EKDQHLVFCEVKTRTSHSIEWALASIGFSKQRKISRTAQLYINENPQFAKHIFRFDVL
                     LVFYYENTDTFEIKHFENAFDAILEY"
     gene            complement(23499..24836)
                     /locus_tag="PLW08_02025"
     CDS             complement(23499..24836)
                     /locus_tag="PLW08_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPL51412.1"
                     /translation="MKNIRLFFSLVVVLFAFSTLLGSDKLNMFIDYNRFLDKNKNTIL
                     LLDYQIPYENLAFLAKNNGYFAELNVKVKITEADSIFLEHEINDIIGVKNKADTGNRK
                     KSYLNRLSFTFHKPLYTIQFSALDINSQKNFFYEFQIETLPPSSLLSDVELNSEVRPD
                     TSQYLVKFKRNNVLYRSEPSILFNKSINDYIYLYLEIYNWNSTSESCLLNLSLEKDSL
                     VVMDEYIDFQPGPGSESINLKIPLKDLNPGFYNGTIVLQSAERTEERNIEFAVIEEKE
                     EEFFLFANPDEEYELMRIFLGNKIPSDWGKMNQDTKRRYITQFWRDMAFSTNRSVQSI
                     MDLVQKRIDYANRNFTHFKQGWTSDMGRIYIRNGPPDDIQRDTSSDESRFVRKDYQIW
                     KYVSGKKPVYMFIDIQMNGNYRLIYVENDDLEASNPDWMRYVGSDFDTSKLDN"
     gene            complement(24823..25929)
                     /locus_tag="PLW08_02030"
     CDS             complement(24823..25929)
                     /locus_tag="PLW08_02030"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPL51413.1"
                     /translation="MEKIISFCLLLFAFSFLFAQEIFYEHYGNEVEIWVVLPYSSINF
                     KKGSDEALYQVSLQISDAKKKQVATLGKSQVIPKCNWLNDCGIPIHFQQELPQGSYNV
                     ILQIKNKAMGERRSYSRQFIISSSSTEIGLSWIIAKREGLEYLPNTLSGLVSDEVKLY
                     QSYSISLDSLKITIDNQVLTVKNPQNPIVLDLLPYLQTDAQNLMNLIFYENNIYYKME
                     PFLFTPWYAYSLRYSLEDQIRQIRYIANQNEWKYLRRLPKEKQAEAIETFWKANDPSP
                     GTVRNELREQFYQRVMIADERFTLHKKIAGWTTDQGRIYIKYGEPDDVYSEVYALDKY
                     PYIIWYYYDKNLEFIFADITGYGQYTLRNKDEEY"
     gene            complement(25913..29353)
                     /locus_tag="PLW08_02035"
     CDS             complement(25913..29353)
                     /locus_tag="PLW08_02035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPL51414.1"
                     /translation="MSFVHLHNHTQYSMLDGACRVDRMIKLAKEYAMPAVAITDHGNL
                     FGAIDFYKTAKNAGIKPILGIEAYIINGDLESETGKNEIRYHLILLAQNEQGYKNLMK
                     LSSYSYIKGFYYKPRISKTLLQQYSEGLICLSACVKGEIPALIYNDRMQQAEEVALWY
                     KDLFQESFYIEIQDHNLEPEKKIMPRLIDLAKKLEIPMVLTNDCHYLNKNDYEAHDIL
                     LCIQTGKLLHDPDRLHYGTNQLYFKSPEEMKELFPDVPEAYNNTLKIADMVDLKLHYD
                     KFLLPNIETPPEFKTMGEYLRFLCYENAKIKYPDLNETIKQRIEYELEVIDRMGFNGY
                     FLIVKDIIDNARKQSVPVGPGRGSGAGSIVAYLLDITQIDPLQYGLVFERFLNPDRIS
                     MPDIDIDFCAQNRSKVIDYIIQRYGRESVTQIITFSTLGAKSVIKDVARVLSVPASEA
                     NAITKTIQGNKTLDEAYNQYPEFSSLINSNDLYRTIFKHSKVLEGLVRQTSVHPAGVV
                     IAPGDLTDYVPLACSTQKGEEKVILVQFEGKWLNDLKLLKMDILGLKNLTLIQKTIDL
                     VKQAQDIDIDINHISLEDKKVYNILGKGETDGVFQFESDGMRKYLMELKPNKIEDLIA
                     MVALYRPGPMQFIDSYIARKHGREKVVYDHPLMENALKETYGVTIYQEQVMQIAREMG
                     GFTGGEADSLRKAMGKKNSDLMQQYQEKFRQGAKAQNVPDAVIEKIWQDWLRFAEYAF
                     NKSHATCYALVAYQTAYLKAYYPVEFMAALLSLEDDPTKIPGKIEVCKKMGIKIIPPN
                     INRSDSEFRVHGKEVLFGLRAIKNLGDAAMRDIIEDRNQNGPYTSIFEFCNRLDSTSV
                     NKTILESLIASGAMDELEGTRAQKWNVIELALQFSSNDQKDRKRGQSTIFDFMDTEDE
                     DNNYNPPLPSVEPWSYIYQLEKEKEVLGFYLSGHPLFEYRSLIKHLTNANSSTGKTKN
                     NGELLIAGIINNISKKKDNKGNPIAFVEFEDLYGKFEVPLFNKDFMQYQKKISPGKVF
                     FIIGNKSTFNGNEDSILRILPNALIDFKELPSELSGEIKINIPYEHLKNGLLTELGNR
                     ISRNTGKFKLIIKIQSEDLNDYQLESRKLFFPDEALLNWLEKEKIDFQVRVATNGKNN
                     "
     gene            complement(29642..30934)
                     /locus_tag="PLW08_02040"
     CDS             complement(29642..30934)
                     /locus_tag="PLW08_02040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51415.1"
                     /translation="MKILFISYFYPPLAGPASLRNLKTVKYLSKAGNTIDVLTVGNIV
                     YNYYDNGLLAESLQNRIIHIPSFDPMSILEKLSGKNKTVSNQIYHNTPERIKLFIRQL
                     YPLDDKVLWLPNLYKAGKKALKDEKYDLLYVSCGPFSSAVAVRTLSKQFKVPYILEMR
                     DYWTLLSDYNLQGTYINRLFSHYTEKKLLQDASLIVTVSKGIAEDISKHFGSFLADKT
                     FILYNGHDEEDFLSLPAVSQDKSKYTLSYFGALYAKRSLKWLLKALKDLQKENKLPPN
                     FSLRLYGNYHIETLQEIKQSGIEEMVKIIPQLSHQEAISAMLSSDALLLLINSDSPKG
                     TLTSKVFEYLRTGKPILALVPKNGEAGKLLQESGQNYICPMESVSAIKICAEKLFADR
                     EKELKFHYPEDKYERRKQVEELNKKLLSVARREGKSFF"
     gene            complement(30931..32064)
                     /locus_tag="PLW08_02045"
     CDS             complement(30931..32064)
                     /locus_tag="PLW08_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51416.1"
                     /translation="MKICIVSNSHPTNDVRLYYKLAMSLAKQNEVYLITTNGIVNNTH
                     NPYQIVVDASSRRLAIWLLGKKVKELKPEIIICVEPLTLLIAWILKKKLKFKVVFDVH
                     EFFALSFSERFPRLLRFPTYLFYQLFLKQLMKIADAVFTVNQEICNQLLGRNKRKPSL
                     VLPNYPVKNVWDYECNIPGSLEQLCQMKFDFIYIGGLTEDRGIFKILKVVSLLKHDFP
                     FLKVLILGKFFKTETEKRFNQSINDYNLNAIIYYQSWIPAEKIGLLLKRCRFGLWFFN
                     PKNRRLRLSTPLKVLEYLSAGLPVITIKTPLMKALIEKNGVGICSAYQSNALADACAK
                     MLKLSDDEYNAMSKKCLELSENKYNWETMEPELFTVINGLSKK"
     gene            complement(32460..33770)
                     /locus_tag="PLW08_02050"
     CDS             complement(32460..33770)
                     /locus_tag="PLW08_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HPL51417.1"
                     /translation="MDRKAKQSAPQSFPEIEESALIFNAEQGKVYHFTKRSIEVIRLA
                     NPYPAAYRKSFETNNWQCYIPSKPFPFLFNKPHINNLSSTIYPIRKNAQNKESVYKKM
                     VAYAGEDKIALLNLFSDRFWFIYCLLMREGNYAVELMNTNPALGYLLSAPAVFHPLKS
                     KKYWQAVNKLVKMKRKDILGYFGFLACETMVKTFAKIKPELCSVDLLVLLRTALQNYP
                     SILRQLSFWEVLNSISLYIYCKGLGEILNHNLIADISTRENEEKKEIIRWIEDICRIL
                     DILKAYKMNVPKHELLTMNDLNAIHDKLLEEMLQIRDVIKNHFPEPPLPDIKCNTLWI
                     EHIQNSAELYIEGSEMHHCIYSYTSNIQQGKCYVAKMLYPERLTIMYKETPKEGLKLI
                     ETHGKCNSKPASESIDLIELWLKGYFISSQEPEQPVALTSIEKL"
     gene            complement(34357..35709)
                     /locus_tag="PLW08_02055"
     CDS             complement(34357..35709)
                     /locus_tag="PLW08_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPL51418.1"
                     /translation="MLKYEYHNLLASKHIRSNLPKEKVLDCIDQVKNAHREIMAEHNA
                     NLLGFYDLPDCSTKHIDDFIANLNPVFDTMLVLGIGGSALGNKALYSALKTEAELPRK
                     LFVYDNVDPVFLYEILSQINLEKTLVNVITKSGTTAETMAGYMIVADIIKRKFPKDYQ
                     KRIIITTDKEKGFLRQVINQEGYPGFTVPDNVGGRFSVLSDVGLLSSAFTGIDIKALL
                     QGAKDMREICWNPNIWENPAYLNGLIHFLYYRQGKNISVMMPYSNSLYDLADWYRQLW
                     AESLGKRKDIKGREIYVGQTPVKALGTTDQHSQVQLYTEGPNDKVFTFLTVEHFKHDY
                     TIPNLHPDREEVNYLGGKKLSELLNAERLATEIALSKAMRPNGNIVFPQIDAYHLGQF
                     IMLYEIQTVFTGKLLCINPLDQPGVEAGKIATYALMNKKGYDQERNEIEQYKKDRGLT
                     "
     gene            complement(35710..37443)
                     /gene="ptsP"
                     /locus_tag="PLW08_02060"
     CDS             complement(35710..37443)
                     /gene="ptsP"
                     /locus_tag="PLW08_02060"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPL51419.1"
                     /translation="MQKLIGTALNNGIFIGRAIHISLPEPEIENGNIKPEDIPREIDR
                     LQNSLSTVEEEINQELHSSNLLPNEAEIISTQREILRDPEIYKQLLTAIQNKLHFATQ
                     AVAECFANIINHFETLKVETFAVRAADYRDVQMRLLNALSGGKNALIHKLAKDQIPVL
                     QEIVPSLVSKLAREGIKAYLVAKGSYNSHSSILSRALGLIAVANIPCLLEAVKDDDLL
                     IVDGYSGLLFINPDEETLDFYKQKTKQEQRKQEQLRKLQSSASKTATGIKIKLLTNIG
                     VLDELEAIKNLSCEGIGLFRTEFLYLGKQSLPSEEEQFAVYKELAEKMNPLPITIRTF
                     DLGGDKLAPLSSEKQEENPYLGNRGIRFSLSYPDIFSTQVRAILRASAFGKVQIMFPM
                     IIDAEDFRKAKRFVQNCQQELSAQGIACKPKIPLGAMIEIPSAALCSEELARECDFLS
                     IGTNDLVQYALAADRNNERVSSYYIQHHPAVLGLIKQTVKSAEKYQTPLSVCGEMASN
                     PQYIPLLIGMGITELSVNPGSFLVAKSIIRKCDAHLFALTEDLNFATPLSAVEHLIEY
                     DLKPYYQNRKE"
     gene            complement(37444..37707)
                     /locus_tag="PLW08_02065"
     CDS             complement(37444..37707)
                     /locus_tag="PLW08_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPL51420.1"
                     /translation="MKQVEVTVTNKLGLHARPSALLVKAATKYRSEFFIEKEGMRVNG
                     KSIMGVMMLAAECGSKLQLIANGVDEDYLLAEIKEMIENGFGE"
     gene            complement(37676..38665)
                     /gene="hprK"
                     /locus_tag="PLW08_02070"
     CDS             complement(37676..38665)
                     /gene="hprK"
                     /locus_tag="PLW08_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPL51421.1"
                     /translation="MKEITVNDFFDAKKKDLALSLITEPETLNKKIGSPHINRPGLAL
                     AGYMEIFAADRIQILGETEVRYLQSLPEEVLISRIRDMFKMDIPCIIVTKGLTLPPVI
                     EYLANELNIALLSSRLSTINLIDNLSRYLREIFVLEKTIHATLVDVFGLGILITGKSG
                     IGKSECALDLVHRGHSLVGDDLITLRYIDDQLIGKAGRDIGHFMEIRGVGFVNAERVF
                     GIAHTRKQKTIDLQIELMPWQENMDYERIGLVNNYAEHLGVKIPIIYLPVSPGKNVSV
                     IVEVAAMNVILKSVGYDAAEDFNRKINDEIRKKTMQKNFEGKGDETGRSDSNQ"
     gene            complement(38883..39419)
                     /gene="raiA"
                     /locus_tag="PLW08_02075"
     CDS             complement(38883..39419)
                     /gene="raiA"
                     /locus_tag="PLW08_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPL51422.1"
                     /translation="MQITITARHFELTKAIRDYVETACSKLNKYFDQIITIHTVLALE
                     NSRNICELSLHAAKFSLQSEAEEMDMYLAIDNAVEKMEGQIKKLKDRITDHQKKALKQ
                     QFETYSRATDFQLDAKNHSRKTIKTKRVVTESLSLSEALEKLEASKEDFLIFKNIESD
                     KINVLVTKDHELYKLLEP"
     gene            complement(39435..40841)
                     /gene="lpdA"
                     /locus_tag="PLW08_02080"
     CDS             complement(39435..40841)
                     /gene="lpdA"
                     /locus_tag="PLW08_02080"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPL51423.1"
                     /translation="MQHCQVIVIGGGPGGYESAIRLSQYGISSLLMEKERLGGLCLNW
                     GCIPTKALVKSAELYSEMLNADNFGLPKPEITLAYNKVWQRKNAVVEQLASGIEFLFS
                     KRDIPVIKATAVSMTKEKGNYIVTTLEGESYSSEYVILATGSETKELPGVKIDEKDIL
                     SSTGILKMQELPKELAIIGGGVIGCEFASIFSAFGVQVTIIEFLPHLIATEEEEISKR
                     LAMQFKKNGVKVITNTGVQEIIKKDKRLELKLNNNTTLTADKVLLSVGRKPVNNLTWK
                     GININEEKGAIVINDFMQTSCDKIYAIGDVTGKMQLAHTASKQGLMAAQHIASRLYPE
                     KYSAPQEPLNYNNIPRCIFTHPEIASVGYTQKDAEEIFGDVRVGKFPFAANGKAVAMG
                     NINGFVKTIARKDTSELVGMHIMGPNAAELIAQGTIMIVNKNKAEDIDSFVFAHPTLS
                     EAIMEAMEDLQSKSIHKI"
     gene            complement(40842..42290)
                     /gene="rpoN"
                     /locus_tag="PLW08_02085"
     CDS             complement(40842..42290)
                     /gene="rpoN"
                     /locus_tag="PLW08_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPL51424.1"
                     /translation="MEQYLSLKQKQELALKPKMLQSLKMLALPILELESYIKQELVNN
                     PLLELREEKDEDDIDESNFEQSTPESSTPIEESDMSENHLETLAEAEELTEILDTWNE
                     YHSSYEGWSSDSETDHTEALIRYEENGKEKFLEQLFPLELPENEMDFITDVVDSCDSY
                     GFLPKGFNIVNLAKKYLITAERAEELHNIILSLSPKGISARNITECLMAQLTEEQKQN
                     RILVGLVSEHFENLIHRRYQKIASAYGVSEETILLAKEQISKLDPKPGLRILSTSAVY
                     VFPDVTMKLIDGKYEIIINDHITPNIIISPRYRKMINRGYFDKETMQYVRERINSAKF
                     LIKSIYMRMRTLERVSLSIINHQYDFFYLGTGQMKPLTYAVIARDLGVNESTISRVVK
                     HKYAETPYGIYAFKDFFTSTAGFDDDYQSVSRQKVKTHLIRLIEMEDKKKPYSDQELV
                     ELLKAEGLNISRRIITKYRDELGILNSHLRRQ"
     gene            complement(42296..43081)
                     /gene="lptB"
                     /locus_tag="PLW08_02090"
     CDS             complement(42296..43081)
                     /gene="lptB"
                     /locus_tag="PLW08_02090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPL51425.1"
                     /translation="MTKKQLSLSYYFMEKHKIRAENLVKIYGKRAVVNDLSLEMSQGE
                     VVGILGPNGAGKTTTFYMIIGLAKPNNGKVFYDDMDISHKPMYKRARLGLGYLAQAPS
                     IFAKLTVEENILAILQTLKISRKEQKNRLAEALEELGLSPLAKQKAYTLSGGERRKLE
                     ITRALVTNPTFLFMDEPFAGIDPIAVADIQDIIAKLRNKDIGIMVTDHNVLETLKIVN
                     RAYIIYEGKIIVSGSSLELINDEKAKELYLGERFLESPFEQRL"
BASE COUNT        13146 a         8360 c         8137 g        13779 t
ORIGIN      
        1 ctttaacaat cttgtaacac ttaagtaaca accccgtaac gaaactacgg aagtgttacg
       61 gaagtgttac ggagtcgtta gggaattgac taatggacaa gcagttacag gtttagaact
      121 aaaaacccgt ctcggcagga catattttgc tataaactac ctgtttttcc cttgttatat
      181 attttccatt atgatagtac atatatagta aaagagaagt gactgtcttc taaattttag
      241 cttgtaaatt taaaagcccg cagggggctt ttttgttgtt tcacagttat tttctgacta
      301 tatagctttt tttacaaatt ttctatttat tactgattta tcaatctgta tattgtatta
      361 ttcctgatga attgttaaaa tatgactttt ccaattgtaa atgattttat tgtgaaaatc
      421 ttaccaccca gctttcatct tcttcatttt tctcaatcac aaaaaagccg tttctcccca
      481 aaagaaacgg caaaatttgt taaacagaaa gcaaaaagct tatctattta cgtttatgac
      541 ggttcttgcg taggcgtttc ttccttttgt gggtggcaat tttatgacgt tttctctttc
      601 ttccgcatgg catagtagat gctcttatta cttggttttt acatttacca cagcgttttt
      661 gaattcgcgg gaaaatttga aggtaggaac ggttctggca ggaacaggaa ctttttggtc
      721 tgttttgggg ttacggccaa cgcgaggttt acgggttttg agtttaaagg ttccaaaacc
      781 acgaatttca atatgattgc ccttagccat actatcttta atggcataca aaagggcatc
      841 aacaacaacg gctacatctt tgcgaatgat tccggtgttt tccgaaataa tttttactaa
      901 atcggctttc gtcatttttc tttctcctta aattagtttt tttcttgctg ttcggcaata
      961 aggtattcat agataaaacc ttaggattta tgtcaaggga aaaatctgat cccgaagctg
     1021 aaaaattgtt caaatccctt ttataataag gcaaaacggg tctttcgtgg gcataaatat
     1081 tttatctttt tgtgccttat tcctgtaact taacctattc caacgagatt gaaaagcact
     1141 atgatgttaa gttcgcaaat tccgattggg tgaagacaga tgtctattga ttttgtggct
     1201 attttaagca cccaagttct atattcgtta gtcgtaaaat aattaaaccc cggagagaaa
     1261 aagattagca atactaaatt tctgcttgac aatgttagca atgctaattt tatggctcta
     1321 tgctatgcac aacaaaatgt aatatttaat atactaaaga aggtgaaata tgcagacggc
     1381 agttcaagat tccgttcccg atgtttattc ctttcggtgt gaaggaaaga aaattatcct
     1441 tcagcattcc gaaaagaagt atattcttta ttacagcaag ggtttgaatt atattcttac
     1501 cttactggaa tatccggaat tgaatatccc ctatcccaat ttggaatcca atctttcttc
     1561 tccggaaaaa gcatacagcc agttttcctc tccccaagaa atttatgaac agcaacttaa
     1621 ggttcaggat ggatttcttg ctgttccaat gactgatttg caaacgatca aagaagtgaa
     1681 agaggaattg attcaagtga ttaacgaact tgccgaactg gaagaaaatt gcgattatgc
     1741 ccgggctgat gaactgcggg atatttatga aaaactgagt aagtatttgc gggatgttta
     1801 tcataaagat tgtaaaatca gacacttcac tagtgaagaa agtaaaatta aaagaagagt
     1861 tgttcgcgct gttaatcgtg ctttggaaga aattgaaatt atagaacccg aactggcaaa
     1921 ggaattaaga aaatcaatag aattcggaaa aacgatttgc tatcatcaaa ggaggaaaat
     1981 tgaggtttcc ggatattgga agtagatata tttcccgcgg attacgcaga tgcatttccc
     2041 gcggattacg cagatttatt ttacgcagat tacgcagatt agattttata attttaagtt
     2101 aaattactat ttagttcctg ggtttcacca ttcactgtta acccgttgac ctattaaccc
     2161 attaactgtt aacccgttaa cctattaacc cattaactgt taacccgttc acctgttgac
     2221 ccattcactg ttaacccgtt cacctgttaa cctgttcacc ccttttactt tttcacttgt
     2281 tttaattcca atttgcgtta tcttattttt tagagtcaat gaaggtagcg caataaaaaa
     2341 atgagataag gagaaggaaa tggatgcctt cagtttttgt aatcccaccc gcatagaatt
     2401 tggggttggt acaatcagaa aacttggaat ggaactaaaa aaagcagata ttaagagttg
     2461 cttaatggtt gccggaggtg gttccataaa gaaaaatggt gtttatgaac aggtttgtga
     2521 gtccttggaa aaagcagaaa tccattttgt agagggttgg ggtgttcaac caaatcctac
     2581 tttggaaaag gtgattgaac tttgtgagct tgccaaacgg gaaaaagtgg aagcagttct
     2641 tggtgttgga ggtggaagtg taattgatac tgccaaaaca gtatctgccg gtgttttttt
     2701 gcaggatatc tggaatgcct tttcgggaaa ggaaaaaatc cgtaatgccc tgcctgtata
     2761 tacagttctt actctttccg ctaccggcag tgaaatgaat ggcaatgctg ttattaccaa
     2821 tacgaaaaca atgcaaaaat ggggtattta ttctccttta atttatcccc gtttgagtat
     2881 tatagaccct tccgtgcaat ccagtttacc tttcaaacaa acagccaacg gagcaatgga
     2941 tgctatggct catattctgg aatattattt tgccgatgat cgtgctatag ctactttggc
     3001 aattgatgat gctttgttga aaactattgt ggaaatgact gaccgcctgc aaaataattc
     3061 cggtgactta ttggcaagag gaaatctggc ttggtgcgca actttagctc ttaatggaat
     3121 ttccgggatt ggattaaaag gtggtgattg ggcttgtcat gatatccaac atgctttttc
     3181 agctttgcat cctgaaattg ctcatggcga agggttggga gttattttcc ctgcctggat
     3241 tgaatatatg agtgaaaaag accctactcg ctttctgcgt tgggctaaaa atgtttggga
     3301 cgaggaaaat gtttcccgtg ctttgcatag atttagagat aaactggaaa gttggggttt
     3361 ggcaaaatct cttagagacc tcggtataaa agaattggaa ttgcctcaat tagttaaact
     3421 gattatgacc agtcccagaa ttggaatggt tagcagattt actgcagtgg aagtggaatc
     3481 actgcttatg ctggcgttct gaagggtgaa aagtgaatgg tgaaacacag gaactaaata
     3541 ttaagttaac ttaaaattat aaaatctaat ctgcgtaatt tgcaagaaat acatctgcgt
     3601 aacccgcagg aaagaaatcc ttttttattt gacacaatct ccctttgaat ttaactggca
     3661 aaaaaaggaa aaaatgatat caacttacag cttatagcat aaacagtaag cataccctaa
     3721 gaaggtaaag aaaatgttag agaaaatact gaagaaagta tttggcgata agaacagtca
     3781 agaccttaag cgctatgaac ccgtagtgca agaaataaat gaaatatttt ccggattgga
     3841 acaatatgaa gatgaacagc taatcgctcg cgttcaggaa attaaacagg aaattgcaga
     3901 taaactaact cctttacgaa atgaacttgc taaactggaa caggaatatc gggaaaccag
     3961 agaagatagc gaaagaaatc gtttagacaa tgcaatagac agcactaaaa aggaactgaa
     4021 aaatcttacc aaaagcactc tggatgatta cctgcccgag gtttatgcca ttgttaaaga
     4081 tacctgccgg cgtttgttag gcaaaaaatt tgaagtccgc ggtcatgaag ttgaatggaa
     4141 tatggttcct tttgatgtgc aattaattgg agctatggct ttacacgatg gtaaaattgc
     4201 cgaaatggct accggagaag gaaaaaccct ggttgctact atgcctctgt ttttgaatgc
     4261 cttagtggga cgtggcgtgc atcttgtaac cgtaaatgat tatctggcaa gtcgtgatgc
     4321 cgaatggatg agtcctgttt ttcaatttca cggactcact gtaggttgta ttactaccgg
     4381 aatggatttt gatgctcgca aagaagcata caattgtgat ataacttacg gaatgaacag
     4441 tgaatttggg tttgactatt tgcgtgataa tatggcaact tctcccgatc agcttgtcca
     4501 gcgagatttc ttttatgcga ttgtggatga agtagatagc attttaattg atgaggcaag
     4561 aacccctcta ataattagtg gtcccattgc ccaggataag aatttctatc acgaattgcg
     4621 tcctgcaatc gcacagcttg ttcagttaca gaattctttg gtacaaaaat acttaagtga
     4681 aatccgagag gatttggatg aaaataatcc ccagtcagat aataaccgcc tggctaaaaa
     4741 cttgcttttg gtgaaaagag ccgctccgcg taacaaagca tttatgaaat tgatgcagga
     4801 cggtgaactg aaaaaaatgg tcaacgatat tgaaggtatc tatttgcggg ataaaaaatt
     4861 gcctgaactg gacaacaacc ttttctatgt ggtagaagaa cgccataata gcgttgacct
     4921 ctgtgaaaaa ggaagagaag tgctttcccg aaaggataaa gaccttttcg ttgtccaatc
     4981 cctggatgaa atgctggcag aaatagataa caatcccgag ctctcggaac aggaaaaaca
     5041 gaaagaaaag tcattgcaga ccaatcgttt tatggataaa agtgaaaagc tgcataacat
     5101 caatcagtta ctgcgcgcct ttaccctctt tgaaaacgat caggaatatg tagtaataga
     5161 taacaaagtt gttattgtag atgaatttac aggacgtcaa atgccgggac gccgtttttc
     5221 cgatggtttg catcaggcat tggaagcaaa agaaaatgtg gaaattgagg caggcacaca
     5281 aacatttgcc actattactt tgcagaacta tttcagaatg tatgaaaaac ttgccggaat
     5341 gacaggaacc gcagttaccg aagaagccga atttatggag atttacaatt tgccggtgat
     5401 ggtgattccg acaaatgttc cggttacacg aattgatcat gatgatctta tatatttagg
     5461 caaaaatgat aaataccagg caatcatcaa tgaaataact tactggcatg aaaggcaaaa
     5521 accggttttg gtaggaactg ttagtgtgga ggtctcggaa attatttccc gtttactaaa
     5581 gcgcaaaggc atagctcata atgttctaaa tgcccgtcaa catcaacgcg aagctgaaat
     5641 tattgctgaa gcgggacaac ctggagctgt aactattgct accaatatgg caggtagagg
     5701 aaccgatata aaacttggca aaggtgttgt ggaaggtact tatgaaagct atctgaattt
     5761 gcctaaaaca ttaaccgccg aatttcctta cggcttacct ttggacggac tccatgtaat
     5821 tggcagtgaa agacatgaaa gcagaagaat tgatagacaa ttacggggaa gagcaggccg
     5881 ccagggtgat cctggaacct caaggttcta tttatctttg gaagacgatt taatgcgcct
     5941 tttcggttcg gacagaatag ctccgatgat ggttaaaatg ggcttaaaat cgggagatgt
     6001 aattcgtcac ccctggatga ccaaagctgt ggaacaagca caaaaaagag tggaagagca
     6061 caactttgaa atccgcagag aactgctgaa atatgacgaa gtaatgaatc agcaacgcga
     6121 agtgatttat gcctaccgtc gtagtgtgct taagggttat gaccttaaaa atgaaattct
     6181 ggaaatgatt actgaatcca ttaccaatat ggtagatgat attattccac cccatagcta
     6241 tcctgaggac tggaatctgg aacgcatctg ccaatggttt caacataatc tgaatttggg
     6301 acttacggtt acagatatag ccagcgacca tctgaataga gaccttctgc tgaatacgct
     6361 tttggaatat gcactttccg cttacgaaaa caaagaaaaa caaatcggca gcgaacaatt
     6421 acgcaatata gaaagaagag ccctcttaga agttgtagat gatgaatggc gtgaccacct
     6481 gcatgaaatg gacttattaa aagacggcgt ctatttacgc gcttatgcca ataaagaccc
     6541 tttaatagaa tataaaaagg aaagctttga actgtttcaa ggtttaatta cccgcattca
     6601 ggaaaatgtt acccgcaaag tgtttactac ttatgttcta tctcaagaac aaattaacaa
     6661 cctgctcaaa aatgcaaacc tgactcatca ggatataaat gctttcctga atgcacaaca
     6721 ggcacaacaa atgactcaaa atatgagcgc tcctcctcaa tataataatc ctgtgggaga
     6781 aattgaaaaa gtccgaccca taaaagtagc tcctaaagtg ggaagaaatg atccctgccc
     6841 ttgcggaagc gggaaaaagt ataaaaaatg ctgcggaata aatgaagcag aatagaattt
     6901 tagaggcctg gaggtcaaaa ggaaagtgaa aaggtaatat atgagtaaag gacttataat
     6961 agtagaatcc cctgcgaaag caggaactat cagtaaattt ctgaagaacc aattcagtgt
     7021 gaaagcatca atgggtcaca ttcgtgattt gcctaaacac gaactgggag ttaatgtcca
     7081 tcagggtttc aagcccgttt atataataga taaaacaaaa agcaaagtga ttgcggaact
     7141 taaagaagca actttatccg cggatagcgt ttatctggca agtgataatg atcgtgaagg
     7201 agaagcaatt gcctggcact tatccgaaac cctgaaaaag gaattaaata ataaacccgt
     7261 ctatcggatt gtgtttaacg aaataacttc ccatgcaatc aatgaggcaa tcaaaaaccc
     7321 cggtcaaatt gatatggcaa aagtggaagc gcaacaagca agaagaatat tagaccgcct
     7381 ggttggttat gaaatcagtc ctttgctgtg gaaagtgatt actaaagacc tttctgcggg
     7441 aagagtgcaa tcggttgctt tgcgtttgat ttgtgaacgc gaggcagaaa taaaagcatt
     7501 tgtccctaaa gaatactgga aaattgaagc ggacttttgg aaaaacaatt tgcctccctt
     7561 taaagcggta ttggaaaaaa tagaaggcaa aaaagtggaa attccggatg agaaaagtgc
     7621 catagaaatc gtggaaaaca tccaaaatgc agaggcaatt ttaagcgaaa taaaacgcag
     7681 caatcgtaat gtagaacccc ttccaccttt tattaccagt accttgcaac aggaagcaag
     7741 taaaatactt ggttttcagg cacaaaaaac aatgagcatt gctcaagttc tatatgaagg
     7801 tattgatttg ggtggagaaa gaaccggtct gataacttat atgcgaaccg attctaccag
     7861 aatggcggaa gaggcaatta ctaaagctaa agaccttata aaagaacgct ttggcaaaga
     7921 actggtgcat cctaaagtcc gagttttcaa aaataaacaa agtgctcagg atgcgcatga
     7981 agcaattcgt cctaccgatc cttccagaac tccggaaagc atagcccagt atttaaccaa
     8041 agaacagatg aaactttata cccttatctg gcagcgtttt atagctacgc aaatgatacc
     8101 ggtaaaactc ctttccacaa gtgtaaagat aaatgctgga aaagcggaat ttgtgtcttc
     8161 tggagctcaa ataatggaag agggtttcct gaaagcatat cctcactata atattccttt
     8221 gggtgaaaag attcatctgg attatgctaa aaatgatgtt ctggaacata gtcctttgga
     8281 aaagacacag cattttacta ctcctccggc aagatacact gaagcatctt taatcaaaga
     8341 actggaggca aaagggattg ggcgtccttc tacttatgct accattatag aaacattaag
     8401 aaagcgtaaa tatgttacta tggagaaaaa ggcatttttg ccgacgcaat tgggaagtga
     8461 tgtaaaccgc tttttggtgg ataagtttga ttatcttttt aatgttcagt ttactgccga
     8521 aatggaaacc aaactggatg aagtagaata cagtaaaatt gcctggaatg aagtagtgca
     8581 ggaatattac gaccaactta ttgccttaat tcgtcaggta gatgtgaaga aggagaaaaa
     8641 caatttcgtt caggataccg gcattgtttg tgatgtttgc aaacagggaa caatgttaat
     8701 caaacacagc aagaggggcg attttctttc ctgcagccgt tttccccaat gcaaaaacac
     8761 gaagaatttt acccacgatg aagatggcaa catcaagatt tttgtgccta cacccttaaa
     8821 tgagaattgt ccgcaatgtg gttctccttt aatgttaaga aacggaaaat atggagagtt
     8881 tattgcctgt tccaattatc ccaaatgcaa atatgcccgt ccgaaaacct taggaattaa
     8941 atgtccggaa tgcggaatcg gtgaattaac agcccgtaaa tccaaaaaag gacgcagctt
     9001 ttattcctgt aatcgctatc cggaatgtaa atatataact aacgataaac cccttccgat
     9061 aagctgtccc aaatgtggaa atccttacat agtagaaaaa tacaaccggg aaaaaggaaa
     9121 aattaaaatc tgccccaaat gcaaaaccga gatggaataa gcaatgaaca ttaaagacgg
     9181 catcaatagc gctttccaaa gcattttcag ccataaactg aggtctttgt taaccctaac
     9241 aggaattgtt attggagttc ttgccgtggt aacaatgttt tccagtgttt atgccataaa
     9301 agcacttatc aaaaagaata tggaaggtat gggctgggat aattctatta ttattttccc
     9361 cggttcagga aatatagatt tggaaacagg aaaaacgcgc agtaaaatta ggcgtgctaa
     9421 acaaaatgtt cctcctttga actatgacga ttatctggca ctgaaagaaa acctgaatta
     9481 caaatgtatt tacgggacaa tctcccaaca tagtttatat cgggttggca ataaaataaa
     9541 taatattatc ttgcgtgcta cagaagtaga atttttccaa aacaaaagct atcctatcag
     9601 caagggacgc tattttaatg cttacgaagc ggaaaataat atcccggttg ccgttttggg
     9661 ttatcatttt gccgaagaat actttaagga taaagacccg attggtcagg ttctggtttt
     9721 aggttcacag cgttttacta tcgtaggtgt tttagcttca gatgttttaa atacaggaaa
     9781 tggaatgaat tttgatccct ggcaaagaga atgggacttg aaagctgtgt atattccctt
     9841 aaaatatgga gctacttatt taagtccggc aagaatgatc catcagattt atatacaatc
     9901 aaattccagt gaggactatg ccaaattaaa aaacgaggca aggcaaatac tgcttgccag
     9961 gcataatatg tatcccaatt ttcagtttat ggatattggc gatatgatct taaatattac
    10021 gcaagagatt aataaattta tggataaatg gaatgtaacc cttattgcca ttgcttccat
    10081 ttctttaatt gtagggggaa taggactttt cagcactctg ctgattagca ttcaggaaag
    10141 aatgaccgaa attggtattc gtaaaagtat tggtgccacc gaacaggata tctttttcta
    10201 ttttattttt gaagcgctgg ctttggcttt tattggagca attttaggtg tagttcttgc
    10261 ctggattttg attgttttga tagctaaagg cattaatttc cccctgtatc ttccagtgca
    10321 gggagttgct gttggaattg gtttttctct attagttggc tttctttccg gaatctatcc
    10381 tgcctggaaa gctaccggaa ttgatcctat tcaggcaatt tactaccacg aatgaaaaca
    10441 ccggaagaac gatttcctcg ttattgctaa ctttgcttct ataaataatg cgttaaaaaa
    10501 taacaaagaa agggagtagc tccagtgaaa aaaaacgata aaatagccga gtttctggca
    10561 aaaaaacgct tgcttttcac acgcatcctg tgtattgctg ttactttgga attcgtcatc
    10621 aattataagg ttcggattca gcatctgcta actaatccct caggtattat catcagtatt
    10681 attctaattg ctttaggtgc ttttatcagg tcctggtctg ccggaattat tcaaaaaggt
    10741 aatcgcctgg tgcaggaagg accttattgc ttaaatcgta atccgttata cactggtagc
    10801 gctttaattt taatcggatt tgttttcttt ttgaatgata tttgggcttg gatagtagca
    10861 attctcttga ttctggtaat tttccctttc accattagca gagaagaaaa aggtctgcgc
    10921 aataaatttc ctgaggagtg gattaactat tccaaaacaa ccggaaggta tttcccccgg
    10981 aaaatttcct ggaaaaaaat gaaatatccc tggtctttcc agttatggct taaaaacagg
    11041 gaataccaaa cctttttcct atctctcctt ctcctgataa taattatcat agttgggttg
    11101 gtttagaggg ttgagaaggt ttagaaggtt tagagggttg aaagccccag atacaacgat
    11161 agcgatgggt gcgtaagccc ctcgtaaaaa aagaaaaata caaccccttt tcagcttttt
    11221 tcccctatta cattgatttt gaagaaaata gcaattcagc cctaagagca ttgctgaagc
    11281 attgcttacc caatactaat gccatccgta atgggtaagc aatgggtaag caatgctatt
    11341 aagaaagagt aagatatggc tagtaaaggg tataagtacc cccctaccag aaatgataaa
    11401 agccaccaag caaaaaacag taaaaataaa aacctatata aatggaagtt attgagatag
    11461 gtagagaaaa tgcaaccgtt atgaatacct atattgaaaa caaaaaaatt cttagtagtc
    11521 aagaagtcaa gacctatcgt ctgcagatgg ttcaagatgc cctaaaatac ggaattaacc
    11581 cttgcgccaa atactagcaa acaacggtta aaatagtgcg caaatgggta aagcgatacc
    11641 agcaaaataa ggaggatggt ttgaaagatc taccccgtaa aaaaacacac tccctaatct
    11701 ccatttggga tagatacatt aaaaagtccc tgctggacga catatttttt actacgggtt
    11761 tcaacccggc gatatgaact caaaagaaaa agataaaagt tccataagaa cggcaggcaa
    11821 agagaataaa gatagataat cttatagaga aaagtagtat gaagcatcag acaagaaact
    11881 tagaagttat acgaagaagg caatatctgt cgtgcctaac ggaacttttt tatgatcagc
    11941 atctgcattt ttgcaccggg ttaaaaaccc gtcgttattt tcctatcatt cctacggaat
    12001 attttctgtt atttatgcgt tatgaaaatg gatagaatat agcttatatt tgtgtttgtc
    12061 attccgtact tgatacggaa ttcattttga atattattat agtatggatt cctccttgcg
    12121 caggaatgac aaggatctga tcattcaaaa aatggtatta tttgcggagg aacggcgtcc
    12181 tccggctaca caaaaaaacg gaggaacggt gtcctccgtt tgtaaaaata gttttgagag
    12241 ttaacctttg cgtaatctga atttgaattc gtaatcaaat tcttcattta tagggaagct
    12301 ccaggattcg caaagttgtt ccagttcggt taaaaaacca ttaggcatat tgccttcagg
    12361 ggtgattatg gcatcctgca cttttccgga ggcgttaatt aacagacgaa tttttacgct
    12421 tcccgaaaaa ggaatagtgg cattccattt catatacaga gattgtatct gtcctttacg
    12481 aatctccaca gctcttctaa cttcatcaat aattgtttgg cgttctgcag gtgcttgaga
    12541 aatggtttct tcagtaactt gagttacttg tttgctttta aactttgcaa gggtttgttc
    12601 tgtgacagct gtcttcccaa aagcaactcc actgggcatc aatttacttt gatctcctga
    12661 agctttaaca atagctaccc cttcgggacg gatttcagaa cccctaactg tgggcgcttt
    12721 ggttgctaca gagccaatat cactggtaaa acttctagtg gcattggggt caaaagttgt
    12781 tccaccaaca cctccaccag gtcctgctcc tccaacacca ggtcctgcac ctccaccacc
    12841 tccacctccg ggacgagaag taacaaatcc ttccataaga gtgagtgcct gaacaggtct
    12901 tgcggctgaa gtggcactcg gatcaaaagt accaaagcca ctaccaaaaa ttgctcctgc
    12961 tgatttagcg gttcccttgc ctccggtaac tccacccggt tgtccttttt cagcagtttc
    13021 ttcaggaact tcggcatatt cacctgcttc ttcaaaagtg ctgggtttcg ctaatgcctc
    13081 tgcttgaatg cgttctgctt tctgaattgt ttgaagtttc tgttccaaag ttgtatcgta
    13141 ggagacctgt ttttttagaa aaagatcaaa gataatcaca aaaataacgg ttagaatgac
    13201 gaacaatagg atcatattac ggctgaattt ttcctcggca gtaggttgag gacgacaggc
    13261 atattgagcg gcaatttgtt gctgttccgg agtaagaaca gaaacataag gttctttaaa
    13321 ttcgtaagaa atttcccaat taggtgcgat ggcaagagtt cctgtcatat cgggacgaag
    13381 caagagttta ttaccctgca gaatgaaact tttagttaga aaagattcat ctaccggttc
    13441 tccatcttta gcacaactta ctttcgcacc aggaggcaac tgcaggtaaa gctgatcacc
    13501 tttttttgtg acaaacaggt gcttttctgg aaatttctca tccaggattt gccagaagag
    13561 gtatttatcc gaaccgataa agaacttgtt cttaaaatcg cgtccataac gcacctgatc
    13621 cagttttttg cctttatagt gcagagttaa gtttaataat ttggacattt tccctccttt
    13681 ttattcagcc aaactaacac tatacttttt aagccaatcg gcatcctcaa gcgtggcaaa
    13741 atatatgtat gagtaacctg ctgctgtagc agcacggaca atttccgtta tatattcaca
    13801 gggaatggaa tagtcggctt gaaccagaag acaggtctct gccttttgat gctgaataat
    13861 gtcctcggct tccattttta gataagttaa aaggtcattg cgtttttggg cgtttgttag
    13921 caaatctgcc ggcgaaccgt attccagggc ttcagttcca acaacaacac gatctgggaa
    13981 tattttaatc ggagttgttc ccgctttttc taaaagctta tcattggtga tggtatgagg
    14041 ataggtaata ttgggttctg cagaaatatt tattacttct gtagagatat ttttaaccag
    14101 gaaaaccaaa ataatggtta gcacatccat caatgaagta atggacaacc cttttgcttc
    14161 ctcaaatttt ttacggacgc gacgttcact tttattagaa gattttagtt tgtccagaat
    14221 ttgcataagt cactccttta ataatactcc actttagtgg cagtataatg aacctgggta
    14281 aatccattga ctttacagat gtctatcgtt ttcatcaggt ctccataaag gacatccgga
    14341 tgaacgagga cagtaatatc gctaagagtt ccttttcggc ttctaatttc ttgcagagtt
    14401 ttattcaggg ttacataatc ataatatcca ttgatggcag agatagtcat cggcttaaag
    14461 cccggttcgc ggatttccat acctaaagta gtattattct cttgggcata ttgttcaccc
    14521 aaaatatgga tttccacttt ttcagtttta gcacctccgc ctccgccacc ttgtccataa
    14581 cttacaccgc tttccccttg cgagaaattt agcgccaaaa gagcaatctg cgaaataacc
    14641 accatcatta gcaacattgg aataatggtt aaaaacagat tcataatcgg caccaggttc
    14701 ggttcctggg gcaccgtttg ggatttttgc tggcgcattg cggatggtct gtattgtctc
    14761 atagttttta atccttaatg gcattgttaa tagtattgat caatttaaca ctaaactcgt
    14821 caatattact gataattttg tttgcccggg ttagcaacca acctttaatt agggttaaag
    14881 gaatagcggt catcaaaccc agagcggttg ttccaattgc ttttttaata ccatcagtta
    14941 aagcagtttg ttgtgctgca ccagttaaac cagctaaacc tgcaaatgct cctaacaaac
    15001 cccaaatggt tcctaaaagt ccaagataag tagctacttg agccaaagta tcaaaccaga
    15061 ataaaccgct atccagttta tacactgttt gcagaacagc ttcttccaga gaattttgga
    15121 cagaatttct caaatcagaa cccgttagac cactttttct agaatctttg aaaaccatca
    15181 aagcgctcca gaagataaag cccatagtag ttcccttata gctttcagca attttagttg
    15241 cttcatccag ctggtcattg cggatataat tgcgaagttt taaataaaat ccagcggcat
    15301 caatcttctc tttgggatat aactgatacc aacgcacaaa agcataaaca agaccaataa
    15361 caagaaccaa caggatagca taagcccaat taccgctatc tttaaatact tgaattaagc
    15421 tataaccctg tttagctggt gcagctggag ctgctgtttc tgttgctggc tgagcaaaca
    15481 ggccaacaaa cataaagagt gagatgatca taaggacgat attttttgtt ttcattttga
    15541 ctccttcagc catttattta gtaatgtatc gctttgttta tgaatatctt caattgtaaa
    15601 ggagaactta tattcccctg ccttccgata ggtatcggat ttatcttgaa aaatatcggt
    15661 agatgggata agagctttta tttcaataac caactctttt tcttccacat atctgcggac
    15721 accttcaaaa ccctttaccg gttgctgttg agtggttgtt gttgctgctt gggcatagat
    15781 aatgcttgta aaaaagagca acaccgctaa taggataaaa acttttttca tcgtatatcc
    15841 ccctttcctg ctttaataat tttaactgtt gcaataaagc cacggtagtt tgaattgtta
    15901 gttactacaa aacgcaaggt attcttgcca gcagttactt ttgctgggtc tatcgtaacc
    15961 tgggatgtat aaaccctaaa cggttcagga tcataatcca gatgaagatt agcagctaat
    16021 tcctgtccat ttagataaac cgaagctact tccggtgcta caaagttgat taaaccgtcc
    16081 cggaactcgg tatccaagta aaaatcaact tcaaaaatag cactgcttac aggtgctgtt
    16141 tcggggaacc aaataggttt ggcaaaagtt tcttccagtc ctacaatgtt atccagggtg
    16201 ataccaaaat tgcctgtaat agttgcagca gaagctgttt ccactcccgt ttcaggatta
    16261 acggttataa ttctccaggt aggatcagta taaagcatca ctgtttccgg aggtatggaa
    16321 gcggctaatt tgttgcggtc tataaagacc tgcaaattca tatataaact ttgctgttca
    16381 ggactatcat taggaacagt caattctatt atgttttgtc cttcaaccca ggcgctggaa
    16441 ggtagtaaat atgctaaacg ggttgtagtt atcggcttat taacatctaa ggtatcggta
    16501 ggaacaacac cgggtttcag atcagtgcca ttcagctgaa ttttaatatc ataaggataa
    16561 actaagtgca ataaagcaaa ttcgggttgg attttagcat taacagtgcg atttagaata
    16621 agttctttat tggaaggtat ttgaacacaa ctcaagcgca cacctttagg cgaagttatg
    16681 ttctgaaccg gtaaatttac atttgtccca tccatttttt cagtccagcc ggaaccaaga
    16741 ggatattcct ccattttata atcgggcaac aaattccatt cctgcagttt ggcataactt
    16801 tgctgcgtat aatagtcggt ataaccagcc agctgataaa agttatagat atttaaatga
    16861 gtagtgtatg ccaaactaaa gaggtcattg ctataatcgg cagccagagt tgcaataaaa
    16921 gcatctaaaa catctttata ctcttgacgg acaccttcta cttcactggc agttcgcaca
    16981 taaactccaa tttgggtgtt aataacatta actccctttt cataaatgcg ggcaattaag
    17041 ttcaacgcgc gcagacggcg ataattttcc aattcaatat tctgtttatc agcatcggca
    17101 agaagcttgt ttaccttggc tacttctgct ttaacagatg ccttgaaatt gggaatacga
    17161 cgataggaac ctccaattaa gtgattctgc caggtagtgc tggcattaac tttgattaga
    17221 gctgcaggag atgatgtcat caaagttccc ttttcaatag cggtaagctg gttgtcaaaa
    17281 tttttcagga aagcgattcg tttctggaga tcgttatatt ctttttccac tgcggcaaag
    17341 tattcataat tcttggtaga gctaaagtcc ttcgtttcaa aatttagtcc ttctttttta
    17401 taaactgctt ccaacctttt atattcatca cgaaacttaa aggcatcggt ataattctta
    17461 ttcttatagg cattatcaaa attataaacc agtttactca gctttaaagt agcaatccat
    17521 tgatagcggt cacccttagt tttgtctttt tggtaaattt ctttagctag ttgctcaaac
    17581 ttaagagtat cccctttagc taaataataa tcagcacttt cctgcataaa gggtatcaca
    17641 ttttcactgt ttggatataa ggttatgaac cggttatagg tttcaactgc tttatccttg
    17701 ttttcagctt cgctgtaaag tatggctgct tttgctaata atgcgtcagg agtaatattt
    17761 ccggtattaa tagttttgtt tctggcttct tcataaagag caagataagc atctgcagca
    17821 gcattcttct gagcaggatt tttttccatt tcctgaatca ttgccatacg tagcttgaat
    17881 gcttgattgc tactgggata tttagcaata aagctttcac gaatgctttt ggcaagttgg
    17941 ggattgttca ttgcagtatc ggcttcggca atttctgctg cacggaaata ataatagtaa
    18001 atttcaccaa tatccgttcg cgttccagca agttctaata gaatatctat tgccttctga
    18061 tattcttttg ctttaatata tgcctcttga gctcccattt tataaccggt aacacgttgt
    18121 gcatccttag aaccaagttc ttctgccaaa cgcaatcgtt cacttgctgc cagagaataa
    18181 tttccagcac tctcggctgc ttcaaagcta ttttgaattt gatagcaaat gttattaatt
    18241 atagcttccc gttcatccgg agtagccgca taaggtaatg ctttacggta atatgtttct
    18301 gcctgggcat aattttgctc attttccgct attgacgcaa gctttccgta aacggtaaat
    18361 tttgttctat cgggaatcaa agtttcattt tccaaagcag agagatataa ctctttagcg
    18421 tcaggcaatt ttccacgatc atattgaata tctgctaaac taagtaaaat attaactgcc
    18481 tgaatttcac gctcaggatt tttataggca tcactgcgca gaacactaat aaatcgctga
    18541 gaatttgact gtaataaagc aaacagctca tccgcattag cgggtaaacc tggctcaggt
    18601 tgaaatcctt ccccataaag gtctttcatc aggttgtaaa tattaaagga ataagtatag
    18661 atcaaacctt cattcaggaa gaaatcctca tcttggggat attttgcatt aaattcctgc
    18721 aggtttctta tcgctgttaa atagtgtttg ggtaaattgg aacgctcggt aagagtattg
    18781 ccaagtagta gtttggtttt atctgcttgg gctttccagg caacataatt ttcaccctgc
    18841 agctccggaa aagcagcata ttgatctatg tgctgataat aagcaagcaa tttggtttca
    18901 ttggcaggat cggcaagaaa ttcgttttgc aggcgaatag ctctcttttc ataagcattt
    18961 ttgatcactg ccagctgagc tgaaatgttg ctgttattca tatttgcttg ataccaggca
    19021 gtgtttttgc catagttggt tataatattc tgatacaaat cttgagctat ttgatcgaaa
    19081 ttctgacctt cccgcagttc atactgagcg ctggcataca attgtaaaat ggaatccact
    19141 aaagtgggat tttccagggc aagaggattt atatctattt tcagccagat aaagtcaatt
    19201 gcttgaatag tagataaatt caacttattt gcagcagctt tatctataat gctgcttata
    19261 atttcaggat tcttgtaact ggcagcaata ttttgcaatt ctgctacacc cttggctggt
    19321 gaatagaaat cgcttccatc caaagcaatc aggcagaaag caatattatc tatagcatct
    19381 tcacggtacc ctttagcgat cataggatca gaaatcttgc cactgtcaac agcattcaac
    19441 aaagttagaa aatcgttcag agccagcata aaatcctgtt cctgaaaaga atttaagcgt
    19501 aaccatccac gctgataaat ggcatcgtat ttatatttac tatccggttt gttaataatt
    19561 tcatcaaagc agtttgtggc taaagcatag taacgctgcg gctgatcagc atcggtagca
    19621 atataataat acaaaattcc cagacggtat agggactcgt cataataagg agaggtctta
    19681 tagttatcta taattctttg ataggaactg atcgcctcgg caaaatctga ctccttatat
    19741 ctgcttgcat catcaagagc tacggcagtc ctgtttattt cataaaacct tgctttatta
    19801 tcgcctatcc tttgatggct aagctgacta ctgataaaac caatattata tagggctgca
    19861 tctatattca cattgttggg atcaagctga acaacttttc tataactttc caaggcaata
    19921 gtaggggtct ccagataaac agcatattga agctcagcca gattataata gagatcagca
    19981 ttactgataa gaaaggttcc atttggctgt tcaactgcct taaaattggg attattgtta
    20041 ataaaatttt gaatacgggt aattaaatca cgcttttccg ccaagatttc tgcctgccgc
    20101 tcagtcagct gttgttccgt ccaattttct atttgcgcga ttcgtgcttg ttccttaata
    20161 tgcaggttgc gataatccag ttgcaattta acaaattcca cattagcaac taaatcttgg
    20221 gcatttttac gaatgggctc attgcgcaca ccactatcag cagttacggc tatttgagta
    20281 gcaatacggt ttttgatttt atttcgttgt tcttctgtga aaatattttt tgattcttct
    20341 tgcataaatt gttcaatctc agccagctga acatctatat cttttttatc ctgagagaga
    20401 gagtaatatt cctgcaaaag agcgttaaag atttgttctt ccacatcgct aatatgttta
    20461 cgagtttgca acaggtcatt tttcctgatc aataaactgg ttcgtttttc ggaataatct
    20521 gctctggtcc ttttcaactg ctcctgaata ttagctaaat caggatccgc aatatcttta
    20581 tccagttcag caataagatt attatagagc tgaatgttct tttcaatatc aaataatcgt
    20641 ttcagttcca tatcactaaa attttgacct tgaacactgg gatcattagt cagaaagcgc
    20701 tcatactgct tttccagaat tttatccatt atctcaagct gtttttggta gttggaagat
    20761 aattgtttta cttgctgaaa taacaaatta ccccttgcca ttgtttcctc agcctgaact
    20821 ttttgatcta tataatcaag aataaccatc tttagtgaat ccagcatagc catttcttct
    20881 gaatgcagat taacgatgga atccagataa aaaatccctt tcaaagaaat taattcgtta
    20941 taattgcggg ttaaatcagc ttttatacca tctatttggg aagctaacct tttatattca
    21001 gcagggctgg gattagcata atagggctct aaatttaagt tctgcactct tgccaggatt
    21061 gccatttttt cgggaagcaa acgattcatt attatatccc ggttttcaat gtaactatta
    21121 agcgtattta taacactttc atattcacca tcatcagcag ccatatcagc ccatatatat
    21181 agataagtag gataatatct atcaatattg taattctcaa atactatagc gcttaagaaa
    21241 tcctttgccg tttttcggtt accctgctcc aaataatgca ccatttgctg gaataaaacc
    21301 aagttcagca tttttcgggc gtcttcattc aaatgagtgt tgagcaagga agtaaaatga
    21361 ctgatagcct tggcattttc tcctacacga acatagaaat gaccaagtaa ataaataaat
    21421 tcattttgat ccatactgga aaaaacatct ttatatttta ccagtgcctg ttcagccagt
    21481 gtcggatctt ccttcaccaa ttctggcagt gcatcaacta aaaacatctt ggctaattct
    21541 ctgatcagct gtttttcctg ggatatttgc tcctggattt gttctttggt atattgagat
    21601 ctatcttgag cataaaccac tgccaagaat gacactgcaa caacaattag gaatataatt
    21661 ttaagattgt gtttcataat cccgctcact tatattctct ttattttccc tatgatgata
    21721 aggttcagga ttttttcact taacaaatgc atcctgaacc ttcttcatca gtatttaggg
    21781 gaaacatgat tcctcaacct acacatttta gatgagtagt ttaagattaa ttattgcttt
    21841 ttcttcaatt cttctatcca tttgcgcagc tcttccattg cttgttgagc tttttgcctc
    21901 tcttctttca gcatttgaag ttctttaagc aaatcttgaa cttcaggaga aagctgttga
    21961 taacctgcag ttccaggtgt aactaaacca ggggtctgta actgggttcc aggaccaact
    22021 acaacttctc ctccagcagg aggtttggct gctgttcctg taggaggaac aacaacaatt
    22081 tctccagtgg gacttacagt agtacctgtt ggaggaacaa ctacacctgt ttcaataggt
    22141 gtttctccaa cctcaactac ttctccgcca gcagatggct ttttcccata actggcataa
    22201 agaccaagat cgggacggcg tttagcttca gcatatttgt caaacaaata ggaaatccct
    22261 aaagcaacac ggatattatt cttataatcg ctatccttga accggtcttc aatagcactg
    22321 gctcccagct taaaaccaaa gtttttatag gcataattta tccctatatt gatgtcttca
    22381 ccatcaaatt ctccctgcaa agtcaaatct ctgaccggtg acatctccac agagaaaaag
    22441 gttcctgtta aataacgcgc tctcggagct tgtcccacaa agcggtttga gccaatccct
    22501 aaattgaaca tccaaggtat tccccaaaag acaacctgtt tagatgccac tgcataagga
    22561 gaatagggct cgtaagaatc agcttccgga ttatcagcaa aatcattatt tacgtccaag
    22621 tattgagctc cgtgcttttc tacaggagat aagatattat ccattcctac ggcaatttgg
    22681 ggaataagta aggtctcctc aataattttc acctttgcat taagtaaata aactaaacca
    22741 tctaaagagg tgtaatcccc aagaaacata ctaagttcca ctctgtccag aattcctact
    22801 cctatcattg tatacggaaa gaaacctttg gaggcatctt tggcgtcttc agtaagtggt
    22861 ttgggagctg atatatcccg ataataacct accaccagaa actctgcagc tttatttggt
    22921 aaaacataag catcgggtgt tcttagcatt cccaaggttt gaaaaggtgc tgcttgagca
    22981 accccaagaa cagcaaccag ggcaattacg gtcaacaaag tttttttcat gtttccctcc
    23041 acctttcggt gttactttct tatcaaagtt catcattctt attgacaata taatcgtcaa
    23101 gctttttttt gctttatgaa aaaatattca cttcaggatt tttctcacat cggcgaagat
    23161 cttgctgctc gctatctcgt aagtaacggt tatactatta cttgccgtaa ttacaggaag
    23221 aaatatggcg agatagatat tattgtggaa aaagatcaac acctcgtttt ttgtgaggtt
    23281 aaaacccgta cctctcactc tatagaatgg gctctggctt ccattggttt ctccaaacag
    23341 agaaaaatat ccagaactgc acaattatat attaatgaaa atccccaatt tgccaaacac
    23401 atttttcgct ttgatgtttt gctcgttttc tactatgaaa acaccgatac ttttgagatt
    23461 aagcactttg aaaatgcttt tgacgctatt ttagagtatt aattatccag tttggaagta
    23521 tcaaaatcgc ttcccacata acgcatccaa tcgggatttg aagcttccag atcatcattt
    23581 tctacataaa ttaaacggta attgccattc atctgaatat caatgaacat atagaccggt
    23641 tttttccctg aaacatattt ccatatctga tagtcctttc tcacaaaacg gctttcatct
    23701 gaactggtat cgcgttgaat atcatccggc ggtccgtttc tgatataaat acgccccata
    23761 tcagaagtcc agccctgttt aaaatgagta aaattacggt ttgcataatc aatccgtttc
    23821 tgcaccaaat ccataatgct ttgaacacta cggttggtag aaaaagccat atctctccag
    23881 aattgagtaa tatatcttct ttttgtatcc tgattcattt ttccccagtc cgaaggtatt
    23941 ttattgccta aaaatatacg cattagttca tattcttcat cggggtttgc aaacagaaaa
    24001 aattcctctt ctttttcttc aatcaccgca aattctatat ttctttcttc agtgcgttcg
    24061 gcgctttgaa ggacaatagt tccattataa aagccaggat tcaggtcttt aaggggtatt
    24121 tttaaattga tgctttcact gcctggaccg ggttgaaaat caatatattc atccataact
    24181 acaagcgaat ccttttccaa agaaaggttt agcagacagc tttccgaagt ggaattccaa
    24241 ttatagattt ccagatagag atagatataa tcattaatgg acttgttgaa caggattgag
    24301 ggctcagaac ggtagagaac attatttcgt ttaaatttaa ccaaatattg agaagtatcg
    24361 ggtctaactt cactgttcaa ttctacatcg ctaagcaagc tactgggagg aagggtctca
    24421 atttgaaatt catagaaaaa gtttttctgg ctattaatat ccagagcgct aaattgaatc
    24481 gtgtaaagcg gtttatggaa agtgaaactt agtcggttaa gatatgattt tttccggtta
    24541 ccagtatcgg ctttgttttt tactccgata atatcgttta tttcatgctc taaaaatata
    24601 ctatctgctt ccgtaatttt aacctttaca ttcagttcgg caaagtatcc gttattttta
    24661 gccagaaaag ccaaattctc ataaggtatc tgataatcaa gtaaaagaat agtattttta
    24721 tttttatcta gaaaccggtt atagtcaata aacatattta atttatccga ccctaagagg
    24781 gtgctaaagg cgaaaagaac taccactaag ctaaaaaata acctaatatt cttcatcctt
    24841 attcctcaag gtatattgtc cgtaacccgt aatatccgca aagataaact ccaggttttt
    24901 atcatagtaa taccaaataa tataaggata tttatccaaa gcataaactt ccgaatagac
    24961 atcatccggt tctccgtatt tgatatatat tctaccctga tctgttgtcc agccggcaat
    25021 ttttttatgc aaggtaaaac gttcatcggc aatcattact ctttgataaa attgttccct
    25081 taattcattt ctaacagttc cggggctggg atcgtttgct ttccagaatg tttcaattgc
    25141 ttccgcttgt ttttctttag gtaagcgtct taaatatttc cattcgtttt gattggcaat
    25201 atagcggatt tgtctgattt gatcctccag agaataacgc agagaataag cataccaggg
    25261 agtaaacagg aaaggttcca ttttatagta gatattattt tcataaaata tcaagttcat
    25321 caggttttgg gcatctgtct gcagataagg aagaaggtct aaaactatcg gattttgagg
    25381 attttttaca gttaagacct gattatctat agttattttc aagctatcca gagaaattga
    25441 ataactctga tatagtttca cttcatcgct aaccagtccg gaaagtgtat taggaagata
    25501 ttccagtcct tctcttttag caataatcca gctgagtcca atttctgtgc tggaagaact
    25561 aattatgaat tgacgagagt agcttcgtct ctctcccatt gctttgtttt ttatctgtaa
    25621 aattacatta tatgagccct gaggaagttc ctgctgaaaa tggataggaa ttccacaatc
    25681 gttaagccag ttacatttcg gaataacttg gctttttccc aaggttgcca cttgcttttt
    25741 tttagcatca ctaatctgca gagaaacctg gtagagtgct tcatcgctcc ctttcttgaa
    25801 gtttattgaa ctatagggca aaacaaccca tatctctact tcgtttccat agtgctcata
    25861 gaaaatttcc tgagcaaaaa gaaagctgaa agcaaacagc agaaggcaaa agctaattat
    25921 tttttccatt ggttgctacc ctcacctgaa aatcaatttt ctctttttcc agccagttca
    25981 gcaatgcttc atcaggaaag aagagtttgc gagattccaa ttgataatca ttcaggtctt
    26041 cgctttgaat tttgattatc aatttaaatt tgcctgtatt acgagatatt ctattaccca
    26101 gttcagttag taatccgttt ttcagatgtt cgtatggaat attgattttg atttctccgc
    26161 tcaattccga aggtaattcc ttaaagtcaa ttaaagcatt tggtaaaatt cttaagatgc
    26221 tatcttcatt gccgttaaag gtggatttat tgccaatgat aaaaaagacc tttcccgggc
    26281 tgattttttt ttgatactgc ataaaatcct tattaaacaa gggcacttca aacttcccgt
    26341 aaaggtcttc aaactccaca aaagcaatag gattaccttt gttatctttc tttttagaaa
    26401 tgttgtttat aattccggca ataagcaatt caccgttatt ttttgtcttg ccggtgctgg
    26461 aattggcatt ggttaagtgt tttattaacg agcgatattc aaacagcgga tgcccgctta
    26521 aatagaatcc caaaacctcc ttttcttttt ccagttgata gatataagac cagggttcaa
    26581 ccgaaggcag tggcgggtta tagttattat cctcatcttc agtatccata aaatcaaaaa
    26641 tagtgctttg tccccttttt ctatctttct ggtcattact gctaaattgc aacgccagtt
    26701 ctattacatt ccatttttga gctctggtgc cttcaagttc atccattgca cctgaggcaa
    26761 tcaaactttc cagaatggtt ttatttacgc tggtactatc taaacggttg caaaattcaa
    26821 atatactggt gtaaggaccg ttttgatttc tatcttcaat aatatcccgc attgcggcat
    26881 cacccagatt tttaattgct cgtaagccaa aaaggacttc tttaccgtga accctgaatt
    26941 cactatcgct acggtttata ttcggaggaa tgatttttat acccatcttt ttacagacct
    27001 caattttacc gggtattttt gtcggatcat cctccaggga aagcaaggca gccataaatt
    27061 ccaccgggta atatgccttc agatatgctg tttgataggc aactaaagca tagcaggtag
    27121 catgggattt attaaaagca tattccgcaa agcgtaacca atcctgccaa atcttttcta
    27181 taactgcatc aggaacattt tgggctttag ctccctgcct gaatttttcc tgatattgtt
    27241 gcattaagtc cgaattcttt ttccccatag cttttcttaa actatctgct tcgccacctg
    27301 taaaaccacc catttcacgg gcaatttgca tcacctgttc ctgatagatt gttactccgt
    27361 aggtctcttt tagcgcattt tccatcagcg gatgatcata aaccactttt tcgcgtccgt
    27421 gtttacgagc tatataacta tctataaact gcataggacc gggtcggtag agagccacca
    27481 tagcaattaa gtcctcaatc ttattgggct tcagttccat aagatattta cgcataccat
    27541 ccgattcaaa ctgaaaaaca ccgtcagttt cgcctttacc caatatgtta tagacctttt
    27601 tatcttccag agaaatgtga ttgatatcaa tgtcaatatc ttgcgcttgt ttcaccaaat
    27661 caatcgtctt ttgaataagg gtaagatttt tcaaacccaa aatgtccatt ttcaatagct
    27721 tcaaatcatt cagccatttt ccctcaaatt gcaccaggat aaccttttct tcaccttttt
    27781 gcgtgctaca ggcaagagga acataatccg taaggtctcc gggagcaata acaactcctg
    27841 caggatgaac acttgtttga cgcaccagac cttccagcac tttggaatgt ttaaaaatag
    27901 tgcgatataa atcattacta ttgattaggg aggaaaattc cggatattgg ttatatgctt
    27961 catccaaagt tttattgcct tgaatagttt tcgttatagc atttgcttcg gaagctggaa
    28021 cactcaaaac cctggctaca tctttaatta cgcttttagc tcccaaggta gaaaaagtta
    28081 ttatttgagt tacgctttct cttccatagc gttgtataat ataatcaata actttgctcc
    28141 tattttgagc gcaaaaatca atatctatat ccggcatact aattctatcg ggattcagaa
    28201 atctttcaaa aaccagtccg tattgcaaag gatcaatttg tgttatatca agtaagtaag
    28261 caacaatact tcctgcaccg gaaccacgac cgggacctac aggaacactt tgtttacgag
    28321 cattatcaat aatatcttta acaatgagaa aatagccatt gaaacccatt ctatcaatca
    28381 cttccagctc atattcaatg cgttgcttta tggtttcatt caaatccggg tatttaattt
    28441 tggcattttc atagcaaaga aaacgcagat attcacccat cgttttaaat tcgggagggg
    28501 tttcaatatt gggcagtaga aatttgtcat aatgcagttt caggtctacc atatccgcta
    28561 tttttaaggt attattatat gcttcaggga catcgggaaa gagttctttc atctcttccg
    28621 ggcttttaaa atataactga ttagttccgt aatgcaaacg gtcgggatcg tgtaatagct
    28681 ttcccgtttg aatacagagc aatatgtcgt gtgcttcata atcgttttta ttcaaatagt
    28741 gacagtcatt agttagcacc atcggaattt ccagtttttt tgccaaatct atcagacggg
    28801 gcataatttt cttttccggc tccagattat gatcctgaat ttcaatataa aaactttcct
    28861 gaaaaaggtc tttataccac aaagcaactt cttctgcctg ttgcattcgg tcattataaa
    28921 tcagagcggg aatttcacct ttaacgcagg cagaaaggca aattagacct tcgctgtatt
    28981 gttgtagcag ggtcttggaa attctgggct tataatagaa tcctttaata taggaatagg
    29041 aggagagttt catcaggttt ttatagccct gttcattctg agcgagtaat attaaatgat
    29101 acctgatttc atttttgccg gtttcacttt ccaggtcgcc attgataatg tatgcctcaa
    29161 taccgaggat aggtttgata ccggcatttt tggctgtttt ataaaaatca atggcaccaa
    29221 aaagattgcc gtgatccgtt atagcaacag ccggcatagc atattcttta gccaatttta
    29281 tcatcctgtc aactctgcag gcaccatcca acatactgta ttgtgtatgg ttatgcaaat
    29341 gaacgaaaga catctctcac tccctgagtt tatctaaaca tataaaaatg gaatacagaa
    29401 atctgtcaaa gaatatctga aagttggcga agttcaaagt gcgggagttc aaagtgcggg
    29461 agttcaaagt tcaaagtgca ggagtgcaaa gtgcaaagtg cattgttatt tttctttttc
    29521 ctctttccct cttttcctct ttgttaaaaa aaatctttct ctctttttct ctttgtaaaa
    29581 ctaaactctt ttcctctttt cctctttgta aaactaaact ctttccctct tttcctcttt
    29641 gttaaaaaaa actctttcct tctcttcttg ccacagaaag tagtttttta tttagttctt
    29701 ccacctgttt tcttctttcg tatttatctt caggataatg aaacttcagc tctttttctc
    29761 tatctgcaaa tagcttttcc gcacaaatct taatagcaga aacggattcc attggacaga
    29821 tataattttg tccactttct tgcaagagtt ttcctgcttc accattttta ggaaccagag
    29881 ccaggatagg tttgccggtt cgtaaatatt caaagacctt gctggttagg gttcctttcg
    29941 ggctatccga atttattaaa agcagtaagg catcggaaga aagcatagcg gaaatagctt
    30001 cctgatgact cagctgggga ataattttca ccatttcctc aattccgctt tgtttaattt
    30061 cctgcaaggt ttcaatgtga taattaccat agaggcggag agaaaaatta ggtggcagtt
    30121 tgttttcttt ctgtaaatct ttcagtgcct tcagcagcca ttttaacgat cttttggcat
    30181 aaagagcacc aaaatagctt agcgtatatt tacttttatc ttgggaaacg gcaggcaggg
    30241 aaagaaagtc ctcttcatcg tgtccgttat ataaaatgaa ggttttatct gcaagaaagc
    30301 taccaaaatg tttacttata tcttctgcaa tacctttgct aacggtaact attaaagaag
    30361 catcctgcaa gagttttttt tccgtgtagt gtgaaaaaag ccggttgata taagttccct
    30421 gcagattata gtcacttaaa agagtccaat aatctctcat ttccaatatg tatggcacct
    30481 taaactgttt acttagcgtt cttaccgcca cagcagaaga aaaaggtccg caggaaacat
    30541 agaggaggtc atatttttca tccttcagcg cttttttgcc tgctttgtat aagttgggca
    30601 accacagaac cttatcatcc aaaggataaa gctggcggat aaagagtttt attcgttcag
    30661 gtgtgttatg atatatttga ttggagaccg ttttattttt cccgctcagt ttttccagga
    30721 tagacatcgg gtcaaaagaa ggaatatgaa taatccgatt ttgcagggat tcggctaaaa
    30781 gtccgttatc ataatagtta tatacaatat tgcccacggt tagcacatca attgtattac
    30841 ctgctttact taaatatttc accgttttca ggttgcgcaa agaagcaggt cctgctaaag
    30901 gtgggtaaaa atagctgata aaaagaattt tcacttttta ctcagtccgt ttattactgt
    30961 aaataattcc ggctccattg tttcccaatt gtatttgttt tccgataatt ccaggcattt
    31021 tttgctcata gcattatatt cgtcatcgct aagttttaac atttttgcgc aggcatcggc
    31081 taaggcattg gactgataag cggaacatat tcctacaccg tttttttcta ttagcgcttt
    31141 cattaaaggt gttttaattg ttattacagg aagccctgca gaaagatatt ccagcacctt
    31201 gagaggagtg gaaagtcgta atcgcctatt tttcggatta aaaaaccaga gtccaaaccg
    31261 acagcgcttg agtaaaagtc ctattttttc tgccggaatc cagctttgat aataaattat
    31321 agcattcaaa ttatagtcat tgatgctttg attgaatctc ttttcggtct ctgtcttaaa
    31381 aaatttaccc agaataagca cctttaaaaa aggaaaatcg tgtttcaata gggagacaac
    31441 tttcaaaatt ttaaagatac cacggtcttc agtaagtcct ccaatataaa taaaatcaaa
    31501 tttcatctgg caaagctgtt ccaaagaacc tggaatatta cattcataat cccaaacatt
    31561 tttcaccgga taattaggta aaaccaaaga aggtttgcgt ttatttctgc ctaataattg
    31621 attacatatc tcctgattta cggtaaaaac tgcatcggca attttcatta actgctttaa
    31681 aaataactga taaaataaat aggtaggaaa tctcaataaa cgcggaaacc tttcactgaa
    31741 agaaagcgca aagaattcat gcacatcaaa aacaactttg aatttcagct ttttcttcaa
    31801 aatccaggca attagcaagg ttaagggctc cacacaaatt attatctccg gtttcagttc
    31861 ctttaccttt ttccctaaaa gccagatagc taaacgacga gaagaagcat caactactat
    31921 ctgatatgga ttatgggtat tattcactat gccattggta gtgattaaat atacttcatt
    31981 ctgcttggct aaactcattg ccagcttgta atataaacgc acatcattag taggatgcga
    32041 gttggaaaca atacatatct tcattatcgg aaactgcctt ttagattatc ataattatta
    32101 tattttctta ttctgctctt ttatgtcaag aagaaaagag tgattttttt acaaagagag
    32161 aaagagagaa agagtttagt tttacaaaga gaaaaagagg aaaagaggaa ttttttataa
    32221 agaggaaaag aagaaaagag gataatttct atgaaccgga aagaaaaaca acatagtcgc
    32281 attagttaaa tccaaatata gcagtcaggg aaaaatgggt tggcaaatta tttctttgcc
    32341 aaagaaactt taggtttctg aataaaataa tgcttttagt ttttgcttaa gttggaattc
    32401 ggcaacccaa ttgcttaata tgtttattaa caatttctaa ggcattattg gggtgtaaat
    32461 caaagctttt ctatagaagt aagagcaacc ggttgttcgg gttcttgaga actgataaaa
    32521 tatcctttca accaaagttc aatcaggtct atggattcag atgcgggttt agaattgcat
    32581 tttccgtgag tttcaattaa cttcaagccc tcttttggag tttctttata cataatagtt
    32641 agtcgttcag gataaagcat tttggcaaca tagcactttc cctgttgaat attggaggta
    32701 taggaataga tacaatgatg catttctgaa ccctcaatat agagttcggc agagttttgt
    32761 atgtgttcaa tccacaatgt attacactta atatcgggta gagggggttc aggaaaatga
    32821 tttttaatca catctcttat ttgtagcatt tcttccagca atttatcgtg gatagcattc
    32881 aaatcgttca tagttaagag ttcgtgtttt ggcacattca tcttatatgc ttttaagata
    32941 tcaagtattc tgcagatgtc ttcaatccag cgtatgatct cttttttttc ttcattttcc
    33001 ctggtagaga tgtcggcaat caggttgtga ttcaaaattt cccccaatcc tttgcagtat
    33061 atatataagg aaatagaatt gagaacttcc caaaaagaga gttgacgcag aatggaagga
    33121 taattttgca aagcggttct gagaagaact aataaatcaa cggaacaaag ttcgggtttg
    33181 attttagcga aggtcttaac cattgtttcg caagcaagaa aaccaaaata gcccaggata
    33241 tctttgcgtt tcattttcac cagtttattt accgcttgcc agtatttttt acttttcaga
    33301 ggatgaaaaa cagcgggtgc actaagcaaa taacccaaag caggatttgt gttcattagt
    33361 tcaacagcat aatttccttc tctcatcagc aggcaatata taaaccaaaa acggtcagaa
    33421 aaaaggttca gtaaagcaat tttatcctca cccgcataag caaccatctt tttatagacg
    33481 gattccttat tttgagcatt tttccgaatg ggataaatgg tgctggataa gttattgata
    33541 tgtggcttat taaacagaaa aggaaagggt ttggaaggaa tatagcattg ccagttattc
    33601 gtttcaaaac tttttctata cgctgcagga taaggatttg ccaagcggat aacttctata
    33661 gagcgtttgg taaagtgata tactttgccc tgttctgcgt taaaaattaa cgcactttct
    33721 tctatttctg gaaaggattg tggagcggac tgttttgctt tcctgtccat agaaggttac
    33781 ctccctcatt attatctctt tggatgctta tttttcggct tttcttctct ttttcaaaaa
    33841 ttactctcgc cccaatgagt aaaaaatgtc aagtaaaaat aagcttgaga aagttgaaaa
    33901 tgtttagata gttgagaagg tttagaaagt tgagattttt tcccgcttca aagagtaaaa
    33961 ccggaaggtt gaattcatcg tcaaccacct tttatgattt atcaatatta aaattacatt
    34021 atattgattt tgtggaagat agcaactctc ccctaagagc attaatttag cattgcctac
    34081 ccattactaa tgccatccgt aatgggtaag caatgggtaa gcaatgctat taaggaagaa
    34141 ctaaaagaaa agtgttaatc gcataatatt ttagtaagtt agaatattat cccgaataat
    34201 acagaagaaa tagatagtaa ttataagtag caagagagat gtaaaaatcc tatttgccac
    34261 agatagcaaa gttatctgcg aagaactctc tacttcatat ttgctgaatc agaatcctgt
    34321 aatacattta ctaaagtgga aagcactgaa tcattttcag gttaagccac ggtctttctt
    34381 gtattgttct atttcgttgc gttcctggtc gtaacccttt ttattcatta aggcgtaggt
    34441 agcgattttt cctgcttcta ctccgggttg gtctaacgga ttgatacaaa gaagtttgcc
    34501 tgtaaaaacg gtttgaattt catagagcat aatgaattgt cctaaatggt aggcatcaat
    34561 ttggggaaag acaatattgc cattaggacg cattgcttta ctgagggcaa tttctgtagc
    34621 aagtcgttcc gcattaagga gttcggaaag ttttttgcca cctaaataat taacttcttc
    34681 ccggtcggga tgcaaattgg gaatagtgta atcgtgttta aagtgttcca cggttaagaa
    34741 agtgaagacc ttatcgttgg gaccttcagt ataaagttgc acctgagaat gttgatcggt
    34801 tgttcctaat gctttcacag gtgtttgtcc cacataaatt tccctgcctt taatgtcctt
    34861 gcgtttaccc aaactttctg cccaaagttg acgataccaa tcggcaagat cataaagaga
    34921 attgctatag ggcatcatta cgctgatgtt tttcccttgg cggtaataga gaaaatggat
    34981 caaaccattc agataagcag gattttccca aatattgggg ttccaacaaa tttcccgcat
    35041 atcttttgct ccttgcagca atgccttaat gtcaattccg gtaaaagcgg aagaaagcaa
    35101 acccacatca gaaagaacac taaacctgcc tcctacatta tccggaacag taaaaccggg
    35161 gtaaccctcc tgatttatta cttgccttaa aaatcccttt tccttatccg tagtgataat
    35221 aatccgtttt tggtagtcct tagggaattt gcgtttaatt atatctgcca caatcatata
    35281 gccagccatt gtttcagctg tggttccgct tttggtgata acatttacca aagttttttc
    35341 cagatttatt tgagacagga tttcatagag aaaaacgggg tctacattat cataaacaaa
    35401 gagtttacgg ggtaattccg cctcagtttt caaagcggaa tataatgctt tattgcctaa
    35461 agcggaaccg ccaatgccta aaaccagcat tgtatcaaaa acgggattga gattagcaat
    35521 aaaatcgtca atatgtttgg tagagcaatc tggcaaatca taaaaaccaa gtaaattagc
    35581 attgtgttct gccataattt cgcgatgagc gtttttcacc tgatcaatac aatccagcac
    35641 tttctctttc ggcaaattac tgcgtatatg tttggaagcc aagaggttgt gatattcgta
    35701 tttcagcatt tattccttcc tgttttgata atagggtttt aaatcatatt caattaaatg
    35761 ttccactgct gacaaaggag tggcaaaatt aaggtcttcg gttaaggcaa aaagatgggc
    35821 atcacatttc cggataatag atttggctac cagaaaactt cccggattaa cgcttaattc
    35881 cgtaattccc attccgatta aaagaggaat atattgagga ttggaagcca tttctccaca
    35941 aacggaaagt ggggtctgat atttttccgc actttttacc gtttgtttaa tcaatcccag
    36001 aaccgcagga tgatgttgaa tatagtaaga tgacaccctt tcattattgc ggtctgcagc
    36061 caaagcatat tgcacaaggt cgttagttcc gatacttaaa aaatcgcatt ctcgggctaa
    36121 ttcctcagag cataatgcgg cagaaggaat ttctatcatc gctcccaggg gaatttttgg
    36181 cttgcaagca attccttgag cagaaagttc ctgctgacaa ttttggacaa accttttggc
    36241 tttgcggaaa tcttctgcat ctataatcat cggaaacata atttgtacct tgccaaaagc
    36301 cgaagccctc aaaatagctc tcacctgagt ggaaaaaata tccggatagg aaagggaaaa
    36361 tcttatgccc cgattgccta aataaggatt ttcttcctgt ttttcagagc tcaaaggagc
    36421 aagtttatct ccccccaaat caaaagttct gatagtaata ggcagagggt tcattttttc
    36481 cgcaagttct ttgtaaacgg caaattgttc ctcttcggaa ggcaagcttt gttttcccaa
    36541 atacaaaaat tccgttctga ataagccaat tccctcacag gaaaggtttt taatagcttc
    36601 cagttcatcc agcactccga tattggttag cagcttaatt ttaatacctg ttgcggtttt
    36661 ggaagcggag gattgcagtt ttcttagttg ttcctgtttt ctttgttcct gctttgtttt
    36721 ttgtttgtaa aaatccaaag tttcctcatc cggattgata aaaagcaaac ctgaatagcc
    36781 gtctacaatt aaaaggtcgt catctttaac tgcttccaat aaacaaggaa tattagcaac
    36841 cgcaattaaa cccaaagccc ggcttaaaat agaagagtgt gaattgtagc ttcctttggc
    36901 tacaaggtat gccttaattc cttccctggc aagtttgctc accagggaag gcacaatttc
    36961 ctgtaacacg ggtatttggt cttttgccag cttgtgaata agggcatttt taccgccgga
    37021 taacgcattc agcaaacgca tttgcacatc gcgataatca gcagcccgca cggcaaaggt
    37081 ttctactttc agcgtttcaa agtgattaat aatattggca aaacattcgg caaccgcttg
    37141 tgtagcaaag tgtaatttgt tttgaatggc agttagcaat tgcttgtaaa tttcgggatc
    37201 acgcaaaatt tcacgttgag tagagattat ttccgcttcg tttggaagta gattactgga
    37261 atgaagttcc tggttaattt cttcttcaac ggtggaaagg gaattttgta aacggtctat
    37321 ttccctggga atatcttcgg gcttaatatt gccgttttca atttccggtt ccggcaaaga
    37381 aatatgaatt gccctgccaa taaaaattcc gttattcagg gcagttccta taagtttttg
    37441 catttattct ccaaaaccgt tttcaatcat ttccttaatt tctgccagca aataatcctc
    37501 atccacacca ttggcaataa gttgcagttt ggaaccgcat tcggcagcta acatcataac
    37561 gcccataata ctttttccgt ttaccctcat tccctctttc tcaataaaaa attcactgcg
    37621 atattttgtg gcagctttaa ccaaaagtgc tgagggcctt gcgtgcaaac ctaacttatt
    37681 ggttactgtc acttctacct gtttcatcac ctttgccttc aaaatttttt tgcattgttt
    37741 tcttacggat ttcatcgttt attttgcggt taaagtcctc agctgcatca tatcccacac
    37801 tttttaaaat aacattcatt gcagctactt ccacaataac cgaaacattt tttccgggag
    37861 aaacaggcaa atagatgata ggtattttca ctcctaaatg ttcagcataa ttattaacca
    37921 aaccaatgcg ttcataatcc atattttcct gccagggcat cagctctatc tgcaggtcta
    37981 tagttttttg tttgcgcgtg tgagcaatgc caaaaacgcg ttccgcattc acaaaaccca
    38041 cacctctaat ttccataaaa tgtccaatat cccttcccgc cttgccaatc aattgatcgt
    38101 ctatatatcg taaagtgatt aaatcgtctc ccactaagct atgtccacgg tgaacaaggt
    38161 ctaaggcaca ttcacttttt ccgattccgc ttttaccggt aatcaatatt cccaaaccga
    38221 aaacatcaac cagcgtggca tgaatggtct tttccagaac aaaaatttct cgtaaataac
    38281 gggaaagatt atcaattaaa ttaatagtgg aaagccggct ggaaagaagt gcaatattca
    38341 attcgttggc aagatattca atcaccggag gcaaagtaag acctttggta actataatgc
    38401 agggaatatc cattttgaac atatcccgaa tgcgggaaat taaaacctct tccggtaaac
    38461 tctgtaaata gcgaacttcc gtttctccca aaatctgtat tctgtctgcg gcaaaaattt
    38521 ccatataacc agccagagct aatccgggac ggttaatatg aggtgaaccg attttcttgt
    38581 tcaaggtttc cggttcagtt atcaaagata gcgccaggtc tttctttttg gcgtcgaaaa
    38641 agtcattgac ggtaatttct ttcatctaag ttcctcgctt catccggaat ggcattttcc
    38701 tttttacatt ctgtagaatc ggaatttatc tatccttcat gagtagcaaa ttccgttttg
    38761 ccacagctaa tgaagtcagt tatttgattc ttcgcttaac tttctcccga aaaggaattc
    38821 tgcaaatctg ggaaaacttt tttgccgaag tgaaattcgg cagacatttt atcggtagaa
    38881 ctttatggtt ccagcagttt ataaagctcg tggtctttag ttaccagaac atttatttta
    38941 tcgctttcaa tattcttaaa aatcaggaaa tcctctttgc ttgcttccag tttttccaga
    39001 gcttcagaca aactaaggga ttcggttacg actcttttag tttttattgt ttttcggctg
    39061 tgatttttcg catccagctg aaaatcggta gcgcgggaat atgtctcaaa ttgttgtttt
    39121 aatgcttttt tctggtggtc cgtaatgcga tcttttagct ttttaatttg accttccatc
    39181 ttttccacag cattatcaat agcaagatac atatccattt cttcagcttc actttgaagc
    39241 gaaaatttgg cagcgtgaag ggaaagttca cagatattgc ggctgttttc caaagccaaa
    39301 acggtatgta tagtgataat ttggtcaaaa tacttattca gcttggaaca agctgtttct
    39361 acataatcac ggatagcttt ggtaagctca aaatggcggg ctgtaatagt gatctgcatt
    39421 gggggttcct cctgttagat tttatgaatt gatttacttt gcaggtcttc cattgcttcc
    39481 attattgctt ctgaaagggt cggatgagca aaaacaaagg aatcaatgtc ttctgcctta
    39541 tttttattga cgatcataat ggttccctga gcaataagtt ctgcagcatt gggacccata
    39601 atatgcattc ctacaagttc tgatgtatct ttacgggcaa tggttttcac aaaaccgtta
    39661 atattaccca ttgccactgc ttttccattt gctgcaaaag gaaattttcc cactctcaca
    39721 tctccgaaaa tttcttcagc atccttttgc gtatagccca cggaagcaat ctcgggatgt
    39781 gtaaaaatgc agcgcggaat attattataa tttaacggtt cttgaggtgc ggagtatttt
    39841 tccggataaa ggcgtgaagc aatatgttgt gctgccatca gaccttgttt actagcagta
    39901 tgagcaagtt gcattttccc ggtaacatcg cctatagcat agattttatc gcagctggtc
    39961 tgcataaagt cattaattac aattgcacct ttttcctcat ttatgtttat tcctttccag
    40021 gtaaggttat taaccggttt tctgccaacg cttaacaaaa ccttatctgc tgtcaaagtt
    40081 gtattattgt ttagtttcaa ttccaggcgt ttatcttttt taataatctc ttgcactccc
    40141 gtatttgtta taaccttcac tccgtttttt ttgaattgca tagccagtcg tttggaaatt
    40201 tcttcttctt cagtagctat aaggtgcggc agaaattcta taattgtaac ctgcaccccg
    40261 aaagcagaaa aaatagaagc aaattcacaa ccgataactc ctccgcctat aatagcaagc
    40321 tctttaggca attcctgcat tttcaaaatg ccggtggagg aaaggatatc tttttcgtca
    40381 attttaacgc cggggagttc ttttgtttca ctgccggtag ccaaaattac atattccgag
    40441 ctgtaacttt ctccttctaa agtggttaca atataatttc ccttttcctt tgtcatggag
    40501 acggcagttg ctttaatcac aggaatatcg cgcttggaaa aaagaaattc aattccgctt
    40561 gctaattgtt ctacaacggc atttttgcgt tgccaaacct tgttatatgc caaagttatt
    40621 tccggtttag gtaagccgaa attatccgca ttgagcattt ctgaatataa ttcggcactt
    40681 tttaccaatg cttttgtagg tatacaaccc caatttaaac aaagtcctcc gagccgttct
    40741 ttttccataa gtaagctgga aatgccatat tgagaaaggc gaatggctga ttcataaccg
    40801 ccaggacctc ctccaataac gatcacttgg caatgttgca tttattgtct cctgagatgg
    40861 ctgttcaaaa tgcccaattc atcacggtat ttagtaataa ttctccggga aatatttaaa
    40921 ccctccgctt taagaagttc taccagttcc tggtcactat agggtttttt cttatcttcc
    40981 atttcaatta agcgaattaa atgggttttc actttttggc gggaaacgct ttgataatca
    41041 tcatcaaagc cagcagtgga agtgaaaaaa tccttgaaag cataaatccc gtaaggtgtt
    41101 tccgcatatt tatgtttaac taccctgctg attgttgatt cgttaactcc taaatctctt
    41161 gcaattacag cataggtaag gggtttcatt tgtcctgtac ccaaataaaa gaaatcatat
    41221 tggtgattga taatagaaag ggaaactcgt tccaaagttc gcattcgcat ataaatggac
    41281 ttaattaaga atttggcgga attgatgcgt tcccgcacat attgcatcgt ttctttgtca
    41341 aaatatccgc ggttaatcat tttacgatag cggggactga taataatatt gggagtgata
    41401 tgatcgttga taatgatttc atatttgccg tctatcagtt tcattgtaac atcgggaaaa
    41461 acataaacgg cacttgtgga aagtatacgt aagccaggtt tgggatcaag cttggagatt
    41521 tgttctttgg ctaataaaat ggtctcttca gaaacaccgt aggcagaagc aattttttga
    41581 taacggcggt gaataagatt ttcaaaatgt tcggaaacca agcccacaag aatgcggttt
    41641 tgtttttgtt cttccgtaag ctgtgccatc aaacattctg ttatattacg ggcagatata
    41701 cctttagggc ttaaggacag aataatatta tgcagttctt cagctcgttc tgccgtaatt
    41761 aaatactttt ttgctaaatt aacgatgttg aaacccttag gtaaaaatcc gtaactatca
    41821 caggaatcaa caacatcggt aataaaatcc atttcgtttt ccggcagttc caagggaaaa
    41881 agttgttcca gaaatttttc tttgccattt tcttcatagc ggataagcgc ttcggtatga
    41941 tccgtttcac tatcggaaga ccagccctca tagctggaat gatattcatt ccaagtatcc
    42001 aaaatttctg tcagttcttc agcttccgcc aatgtttcca aatggttttc gctcatatcg
    42061 ctttcttcaa tcggagtgga actttcagga gtggattgct caaaattgct ttcatcaata
    42121 tcatcttcgt ctttttcttc ccgcaattcc agcaaaggat tatttaccaa ttcctgttta
    42181 atatagcttt ccagctccag aatgggtaaa gcgagcattt ttaaggactg cagcattttg
    42241 ggtttaagtg ccagctcctg tttttgtttt aaggacagat attgttccat tgagctcata
    42301 atcgttgctc aaaagggctt tccagaaatc tttcgcccaa atataattcc ttggcttttt
    42361 cgtcatttat cagttccagg gaagaacctg aaacgataat ttttccttca tagataatat
    42421 aagcccgatt tacaattttc aaagtttcta aaacattatg gtcggtaacc attatgccta
    42481 tgtctttatt tcttaatttg gcaattatat cctgaatatc tgctaccgca ataggatcaa
    42541 ttccagcaaa gggttcatcc ataaaaagaa aagtaggatt agttaccaaa gcgcgagtaa
    42601 tttccagttt tcttctttcg cctccggaaa gagtgtatgc cttctgtttt gccaaaggag
    42661 ataaacctaa ttcttccagg gcttctgcta aacgattttt ctgttctttc cggctgattt
    42721 ttagggtctg taaaattgcc agaatgtttt cttccacagt taatttggca aaaatggaag
    42781 gtgcctgagc caaatagccc aaacctaaac gcgctctttt atacattggt ttgtgagaaa
    42841 tatccatatc atcgtaaaaa accttgccgt tattgggctt tgccaaacca ataatcatat
    42901 aaaaagtggt ggttttccca gctccattag gacctaaaat gccaactacc tctccctgac
    42961 tcatttccag agaaaggtca tttactacgg ctcttttgcc atatatcttt acgagatttt
    43021 ccgctcggat tttatgcttt tccataaaat aataagataa agaaagctgt ttttttgtca
    43081 agaaatactt gcaagtggat tgaattttgc taatatagga cggatagcag ggaagaatga
    43141 attaagtcag gactcgtcac tgccaaaata acttttaatc tatttacgaa tttgtttggc
    43201 tgggaggact cgtgacggca aaaaaacctg atttttaaat tggcaactat cggtataata
    43261 tccttgttca tccgctttaa tcttttaaat acaagtcctg ataaatctgt aaaatctcca
    43321 tacttgatca ttttacaacc aagtaacact caagtaacaa ctccgtaacg agacaacgga
    43381 agtgttacgg aagtgttacg gagtcgttac ttaatcgtct at
//
LOCUS       DAOOZV010000010        41480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1085_length_41480_cov_59.775450, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000010 DAOOZV010000000
VERSION     DAOOZV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 41480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41480
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1085_length_41480_cov_59.775450"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..760
                     /locus_tag="PLW08_02095"
     CDS             278..760
                     /locus_tag="PLW08_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPL51426.1"
                     /translation="MEIITEALKFPSDCNIILGQSHFIKTVEDLYEALVTSVPEIKFG
                     LAFCESSGPCLVRKEGTDNELIEIAVENMFRLGAGHSFLIILRNAFPINIMQAVKDCR
                     EVVNIFCATANPVEVILAQTEQGRGILGVIDGFSPKGIELDTDITHRKKFLLDIGYKR
                     "
     gene            763..1344
                     /gene="thpR"
                     /locus_tag="PLW08_02100"
     CDS             763..1344
                     /gene="thpR"
                     /locus_tag="PLW08_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPL51427.1"
                     /translation="MMIRTFIALELPKPLKSELGSVVNRYSKATPPYVNWVKPENLHL
                     TLLFIGDVQPQEIRIIDEVLAKQLEGFPSFIFRPEGLEFFPSLQPHLLWLKLSSENED
                     IFKLNRRLLKELSACGIEADKKAMKLHITLARLKSSLNPALEREIMSRNIKHEPLYFD
                     CLCLFKSQLFPEGPQYTVLNRYNLIHSRRINAG"
     gene            1334..2389
                     /gene="recA"
                     /locus_tag="PLW08_02105"
     CDS             1334..2389
                     /gene="recA"
                     /locus_tag="PLW08_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPL51428.1"
                     /translation="MLDKNKDAALKTAISQLEKKYGVGTLMRLGDKPHQVVDVIPTGA
                     LNLDIALGIGGIPKGRITEIYGAEASGKTTLALHIAAECQKQGGIVAFIDVEHALDPI
                     YAKRLGVITDDLLLSQPDGGEQALEITETLVRSSAVDLIIIDSVAALVPKQEIEGEMG
                     DSHVGLQARLMSQALRKLTAIVSKSNTAVIFINQTRMKIGAGPYMNPETTTGGVALKF
                     YASVRLEVRHGAQIKDVGSESQVIGTRTKVKVVKNKFAPPFKTVEFPIIFGQGISKLD
                     IILEMAVQHDIIKKSGSWFSYNDLKLGQGADKTKLYLTENQPLLQEIEAKLREKINPE
                     EFNLSAEENVPEDNEES"
     gene            2349..3002
                     /locus_tag="PLW08_02110"
     CDS             2349..3002
                     /locus_tag="PLW08_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPL51429.1"
                     /translation="MLKKMFLKTTKKAEHDKNSLIIIDNEVRGILPDRILLSYFPLPF
                     AGEIDDEQGKELLSLIAKNARQQLLKYVSDQEHSSVQCRQFLSRKHYPAELIDNLIKE
                     FQNKKYIDDFRFVQILISSLIERKKSKRAIAQKLKETRLPNELWERKLNELYPLEEEK
                     ENLKEQIVKLRFRYRDLPLNKQKEKVFASLYRKGFDLDAIHSAWQECSSKGFLPSQE"
     gene            3279..11633
                     /locus_tag="PLW08_02115"
     CDS             3279..11633
                     /locus_tag="PLW08_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPL51430.1"
                     /translation="MKRLFLAILALTLSIGGILSAQALVNIGSGTAVNTTSGVPTPYG
                     TFYKNFRQQYLILASELNDAGGGAGDITSIAFNVSNLNNCSPMPNYTIRLKHTTQTTL
                     TTTFEVGDYTTVWTAPEFMPTTGWNVHNFTTPFNWDGASNLLVDIVTTLIPGSFTQNA
                     SVFYSTTTFNSSLRFQSDSADASSSATGTTSVNRSNMQFIMAPLIVTNPPNPAILVSP
                     ANGATLVSPSANLNWVSGGGAPSGFKLSLGTNNPPTNILNNQNLGLVTTYDPPQDFDL
                     NTTYYWKVTPYNAMGDAVSCPVWSFTTHGAPIVNTLPYMQNFDISVTPPALPFDWTSI
                     VQASVTTAYVDTYASTTYAHSQPNCVRLYNSTDASATVMLVAPVLGDALDPHSVRIKF
                     WARSSSAGYPLSVGVMTSPTDPATYQEVQNIALTATLTEYVIDLTTYTGNGRFITFKH
                     GLGGTSRTLYLDDISFELIAPNDLAALSITGNVTPSVGNATNYTITVKNWGTASQSAY
                     IVKLMSGDTELASAPGPTIAAGATATAVVTWTPTAEGPMSIYGKVVLGSDVNPLNDNS
                     PVMNISVMPPGATVITIGEGNLNEGVPWEFFYKNSLFQTLYYANEIGTIMGNITAVTF
                     YNNFTSNLIDKPVKLWLGTTDLANLSSGWILDGLTLVYDGTLNFPNGQNTIVVPLQTP
                     YLYTGGNLVLYANRPMDTQYYLSTDDFKAQTVGTNRARKLYSDSVTYDPMSPSAAGTL
                     SGTFPKTSLTFVVTGMGSLSGTVTSSGSPVADVLIQVSNASYSFTRYTNAAGEYSFQY
                     LPIGNYTVTASKLGYETQSVTVTIIENQNTVQNFSLVSSNTVSVSGHIVGSDQPTVGI
                     ANAHIFLDGPLDYEGISNANGDFTITGVLSGNTYNYTIQAIGYADLTGTVVVGSTNVN
                     MGTLVMSELALPPVQIVAEENAAQTQVTLTWRPPGSTGTGVGTEDFEIDDGGWVSSGY
                     GDWQWGHYNVANYVDIDTYTDTPPQSAHSGTGMWGTVLEGGYSNCNTWSYLRKTFNLQ
                     SVSNPVLSFWHYMNGYNTWDYGLIKVNGNTVWGNSSSAVFMPWQELTINLSAYANLSE
                     VEISFEWFATGTVSYAGWYIDDLYVGPSMNKTVNYTYVPIPEKAHLLSEEEEALLQPK
                     DFPPVNRSFSFPNSQISNPNRALNGYKVWRFIFGNENNENSWTLLTANTITDTTFIDT
                     AWGTLPDGNYRWAVKGVYTNNLLGPAGFSNRILILRNDLAANTITGNTTPSVGTSFNY
                     TVTIENMGTETKPAGSYTVKLMSGDTELASVLGPTIAAGEELAVTIPFTPTTEGTMSL
                     TGKVVLPADTNPSNNITPPINLTVMPAGILAVTIGEGGQLVGIPWEFYYKNSLFQTLY
                     YSNEIGLFGNITAISFYNNFVSNLTDKPVKLWLGTTNLQDLSGGWILDGLTLVYDGTL
                     NFPSGQNTITVSLQTPYLYTGGNLVLYANRPMDTQYYNTNDDFQAQTIGSNRALKIQS
                     DSVTYDPMNPSVAGTLSGQFPKTTLFFSSPGPNPIFSVGPSSHNWGTVLINTVNEQNF
                     VVGNAGGGTLTINSITISGSEMFSLQNLPALPANLAFCETINFTARYNPTAVGNHTAT
                     ITITDNLATTYTITLNKSNNDGDKRISHNVSLSGNCIDTTLNILPYAQNFDQVTVPAL
                     PLDWMKIVQSTSTSAVVETYASTTYAHSQPNCVRLYNPSDANATLMLIAPPLGTAIPT
                     NTTRVKFWARSSSAGYPISIGIIVNPTDPSTYQETELISLTTTLTEYVVAFNAYTGTG
                     KHIVFKHGLGGTSRLLYVDDVMIEIIPTNDLAATAISGNVTPSVGQESQYNISIHNWG
                     TASQNTYSVKLFSATGAELATAAGITCAPGATVDVPVNWTPTTEGAMTIYGKVVLVGD
                     QNSLNDQTPNLNILVNPSGVFMITIGTGGQFARMPVDMYYRNSIYEGLYYPAEMSNFM
                     GQITGIQFYNNFYTDLPNMPTKVWIGTTTLTSLSDAWIPVSELTLVFDGNINYPAGDN
                     IITIPFNAPYLYLNGENLVIFVQRPFEDVYHNSSDDFHAQTDATYTGRSRRMYSDSVT
                     YDPNNLPATGATVSGQFPKTTLIVIPGGVGHLEGTVVATGSIPLEGVAVNIATTTYGA
                     VTDAQGHYHITNIIPDDYTVNFSKYGYISQSINITIEEDETEILNVTMQPMPTVSVTG
                     TIVASDTGAGINGASIYLVGYQNYNTTSSATGAFSFPAVYANQTYEYTIIATGYTTVT
                     GTINVGATNYNMGSITMNEVAYAPFGVVAALNDIYTAVNISWQAPDPTAVDVTEGFEG
                     TVFPPLQWTQIINNTGPEIIPGVLPTWCRFGTVNPGGNPVAPPQGNYQAGLWWDYEHQ
                     DEWLITPTFNCPPMAYLTVDAYVYRGSIYNDHYYIKVSTDNGTNWTILYDATAATGGW
                     NYYVSPIYLNMEAYGGQQIKIAFQADDPPSNDGLWYCWFIDNLYIGNAVQGVKFAGSD
                     LLPSRSKVAGEKVSSELIVAPYPSREAIEGKRELPTVLKDKMTTPTRSERSLQGYKVW
                     RLTSGNETQPDTWTLLTTQLITTLATVDSAWTALPNGSYRWAVKAVYTANVTSVASFS
                     NVLNKETQMGNIVGFVRKQNNQPIAGATVTAGGISATTNSAGAYSLALSIGTYSVTAS
                     AAGYQPRTIDGVVVLPNQNTTLNFVLDPVANEDEYLPAVSTELIGNYPNPFNPETTIA
                     YSIKDRCKVHLEVYNLKGQLVRTLVNEEKANGHYKAVFNAKDEKGNTLSSGIYFYRLQ
                     AGNYVSTRKMLLME"
     gene            11960..13420
                     /locus_tag="PLW08_02120"
     CDS             11960..13420
                     /locus_tag="PLW08_02120"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPL51431.1"
                     /translation="MMKMWKQIVLVIVALFLMSCAIAKDNMIDPNAPNPVVEVVKNVR
                     DAVVQIRVEAKVTVQNYINPFFNDPWFRQFFNFPQEQTRPIVSLGSGFIYEYNPNTRE
                     AFILTNNHVVEKGREGTITVTMADKVVYTATVVGLDPNTDVAVIKIVVKEGEKVTVAP
                     LGDSDKLQIGEWAIAIGNPFSEGLDRTVTLGVISATSRANLNLGDNAPIYQDFIQTDA
                     AINSGNSGGPLLNIKGEVIGINAALASTNGGNVGIGFAIPINLAKRVVEDLVASGKVT
                     RAYIGILAQEITQDIMESYGLKEVAGVLVAKVEKDSPAEKAGLQVEDIILEINGQKIT
                     SVPKFRIAIATAKAGQEVALKILRNKKEMTVKVILEAYPEDTIATADTAKGTLATGIT
                     VEAIDSPTAKRLNVKSDKGVVVTKVEPNSVAAKSGLKVGYVILKIEGKEVNSPKEFNT
                     ELEKAKTNMEKENRKTIRLYVLDTNNQPSILILKFE"
     gene            13608..14129
                     /locus_tag="PLW08_02125"
     CDS             13608..14129
                     /locus_tag="PLW08_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPL51432.1"
                     /translation="MKKLNSANYKDVNFCLRCGHKLELKTDHENKLRPHCPACNWVYY
                     KNPIPAVAIVLFNENRELLLVKRGLQPKAGFWALPSGYMEINLTPEENALQELEEETG
                     LKGKIMHCVGWFFGKSPIYERVLSIGFRMKAIGGKLQAGDDAVDVKFFPLNNLPVIAF
                     DAHRDFIAKETKL"
     gene            complement(14147..14416)
                     /locus_tag="PLW08_02130"
     CDS             complement(14147..14416)
                     /locus_tag="PLW08_02130"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HPL51433.1"
                     /translation="MLKLSYTSQFKKDYKRYEHNKTVIDALQEALNKLITEIPLPAKY
                     KDHQLKGNLNNCRECHLTPDVLLLYRIQDEVLILVRIGTHSNLFN"
     gene            complement(14418..14675)
                     /locus_tag="PLW08_02135"
     CDS             complement(14418..14675)
                     /locus_tag="PLW08_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51434.1"
                     /translation="MLNKSISFGTTTNTANQISYLDYTNSSCRFSGFFSYNQEIFLKL
                     FTVSKQPQPNKKTLTAIEESKHPDNLPVFNTIDELLKNLEN"
     gene            complement(14665..15657)
                     /locus_tag="PLW08_02140"
     CDS             complement(14665..15657)
                     /locus_tag="PLW08_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPL51435.1"
                     /translation="MRAKSSRENLLKSLEKLRKTKILVYVTGDRPGWETQIHQEVHSF
                     FAHHLERMSKSSKLPKLSLFLYTRGGNTLAAWSLVNLLRQYCNELEIIIPSKAHSAGT
                     LMCLGSNFIVMTKQSTLGPIDPNINTPLNPAIPNMPGHTIPVSVEAINGYIELVKKEF
                     GIKNDQALAEVVKVLSEKIHPLVLGEVYRARAQIKMLANKLLDFHIPNGNKEKIVNFL
                     CTDSGSHDYTINIEDAKDLGLQVEEADKRNAELINSIFETISRQLELNIPFDPKKLLN
                     NKDMVDYNCKRALIQSIEGGKHLFISEGTLTKQCNETNITIHNTLTFEGWRYVK"
     gene            complement(15837..16688)
                     /locus_tag="PLW08_02145"
     CDS             complement(15837..16688)
                     /locus_tag="PLW08_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51436.1"
                     /translation="MLKKLIIFLILATAIALFALANRPPKEFVLQTDVLNEASGIDFG
                     ILNKQVLFSHNDSGNKAEIYALTIDGKLLSTLILKGTKNRDWEDIVVAKDPQNDISYI
                     YIADIGDNNAKYNTVYVYRFKEPQLTGKNEILQISAVDTIEISYEDGPRDAEAIFLEP
                     KTGDIYIISKREEQVGLYRVPYPFNFKDKNNARRIASLPLSWVTAADLSNNGKLLLVK
                     TYTGIYRYKIMLDDKNIIILSKEAKTMPYITEPQGEAVCWDAKNKGYFTLSEAAAGIN
                     QTLYYYK"
     gene            complement(16763..17125)
                     /gene="rnpB"
                     /locus_tag="PLW08_02150"
     ncRNA           complement(16763..17125)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW08_02150"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17282..18013)
                     /locus_tag="PLW08_02155"
     CDS             complement(17282..18013)
                     /locus_tag="PLW08_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPL51437.1"
                     /translation="MEEQLRTLAQMQTLDDEIGRYRVLQKELPKQLNEIIESVEQATA
                     NLLEAETQRAEINKKQRTLEMDIKQLQEQHKKYTNQLAEIKNNKEYKALNSEIAYIKD
                     KISDLESNLLELMDQENEIKEKIASAKAELEKAETRKKEKETDLREQIASLETKIEDV
                     RNKRNDLARTLPVPVVKHYGNMIKHKNNQAVAYNRNGSCGACGFVIRQQMRIELQLRR
                     KIIYCENCGRILMNRFEDNSEDASN"
     gene            complement(18017..18838)
                     /gene="nadC"
                     /locus_tag="PLW08_02160"
     CDS             complement(18017..18838)
                     /gene="nadC"
                     /locus_tag="PLW08_02160"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPL51438.1"
                     /translation="MFIDEIIRKGLEEDIGSGDITTRYLDLEPQNNIAYIIPKEEGVL
                     AGVDVARLVFRMVDPELKIVLYNKDGDKIMPNEEIMRLEGRPSSILQGERTALNFLQR
                     LSGIATKTAKFVSLLEGTNVKLLDTRKTTPLLRSLEKYAVRVGGGYNHRFGLFDMVLI
                     KENHIRACGSITEAVNRIRKHNTSYKIEVEVTNLKEVDEAAKAKVDRVMLDNMTPAQI
                     RKAVKKYKDILEFEVSGGITEENIRSFAETGVQFISSGALTHSYKSLDISLLFKE"
     gene            19196..19271
                     /locus_tag="PLW08_02165"
     tRNA            19196..19271
                     /locus_tag="PLW08_02165"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19229..19231,aa:Ile,seq:cat)
     gene            19320..20264
                     /locus_tag="PLW08_02170"
     CDS             19320..20264
                     /locus_tag="PLW08_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51439.1"
                     /translation="MKSQVLVICLLLITIPLTATKYAGEIFAVSPGVLSTAMGSTGLT
                     YVGSLSAGWWNPALLALNPESGIELMRCEHFEGLMAQNQLSLIIGSKTKTSFTFNHLA
                     IDKIKLTKLENPDDSLSNENRPVVWKTISNQDFILYAGLARQLKKTLYLGISPKLAYR
                     SLAENSGYAFGADLGMFWQINKQANLGVNLRDFFSTQVLWEGGENETVIPNLDLELSY
                     DFSPIKNIPVHLAVRSEILLENREATVETGDLSADFHAGIAVQPISNLCLLAGYDIDC
                     FTAGIGVSYKRLGLNYAFRNGSEDDLGYSQRISVSYQW"
     gene            20258..21403
                     /gene="dxr"
                     /locus_tag="PLW08_02175"
     CDS             20258..21403
                     /gene="dxr"
                     /locus_tag="PLW08_02175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPL51440.1"
                     /translation="MVKKSIALLGATGSIGTSALAVAEEQNVSIVLATAQKNYSSLLQ
                     KAYQHHIPCLIFTGIEDKELQTKLKSENPDKRIYFGEAELLKAIANENYDIALNAIAG
                     SAGLRYSYAILQNNKTLALANKESLIMGGHILTKMLAGKPILPVDSELSALFQAIGNH
                     PAEEIRFLHLTASGGIFRDLPFEDFNQISPQQALKNPNWTMGAKVTLDSATMFNKALE
                     AMETHWLFNQPYSKIKAVIHPQSIIHSLVEFIDGSFLAQMSKPDMKLPILYALSYPQR
                     VESQLVKTDLMSLPPLTFQEIDPQRYPLFYLGLEAAKAGGIYPTVINSAVEAVSVLFL
                     QNKIPYLKMFDLVKKALDEQEPIPDPDLETILQINAQTYQKVLKYLI"
     gene            complement(21945..22385)
                     /locus_tag="PLW08_02180"
     CDS             complement(21945..22385)
                     /locus_tag="PLW08_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL51441.1"
                     /translation="MREKIFSNIVGIQREMLKLLGEVSALTNSPLAIENAIDDVWHPK
                     CDVFQTDTEWIVIAELAGVEKDEISVSISPEYLRISGNRGFPNSNTTVCYYNMEIETG
                     RFDRKIFFPDLNIDKDNPKITYINGILRIAFNLAPIVERIIPVQ"
     gene            complement(22405..23739)
                     /locus_tag="PLW08_02185"
     CDS             complement(22405..23739)
                     /locus_tag="PLW08_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPL51442.1"
                     /translation="MKNFLTVIILLLFALPLLAEDWLTIYNNDLSLVRSRFELELENG
                     RQDINFSDITSRIDPASVIVTGNGIRIAEQNFEYDLAGKWQIMAKYLDREVLIITKDQ
                     SRLTGILKFYSDNSIGIIEKGTERLLVISESETQWIQLAELPDNFYTKPTLHWSIIAS
                     KKAKYPVQMSYLTGGLSWDVTYNTVWDEKTLQFNSWVTINNTSGKAFNDVMLKLIAGD
                     INRVYSGYYGKRGSLISYDAGMAPESAAPSFEEKAFHDFHMYTLDQKVSFANNQTKQL
                     ELYPPKNIKAYAEYQYPTYAANVKSIIKFKNTAENGAGMPLPKGIIKVYKQDSDGNLE
                     FIGEDSINHTSRNEEVSINTGTAFDLIASTRLKEQTQISKYITERLIQITLRNNSNKN
                     KTIKVTHQLSPNTRIVEADYKYTIDNNDKVTFSIDIAPDKEIVWTFRERSEY"
     gene            24046..25317
                     /locus_tag="PLW08_02190"
     CDS             24046..25317
                     /locus_tag="PLW08_02190"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPL51443.1"
                     /translation="MASILVLGSMWGDEGKAKIVDYLASSADYVVRFQGGSNAGHTIV
                     SGGKKYVLHTIPSGILYPQTKCLIGAGVLIDPPAVIKEIKTLEQAGINFKNRLLIDLR
                     AGIVLPLHKQIDLEIETKRRGMQIGTTLKGIGPAYSDLTARYGIRMEDLANPITLKYR
                     IKQLYGYHHIGIGKQDLDILVEKLHKDWKYLKKYVGDVEILLNNIAVKEEKTILFEGA
                     QGTLLDLTFGTYPYVTSSRVMTDGVGIGTGFSARRLNKVLGVYKAYATRVGIGPFPTE
                     MIYEEEVDYIREIGNEYGSTTGRPRRIGWFDAVAGKFSARINALDNAVLTCLDVLTGI
                     EELKICTGYKYKGKIHCSLDLNELQLILSEPVYETFDGWDTDISNCKNINKLPLTARL
                     YIKAIEDHIQTPISLVSVGKERHQTIEIKPL"
     gene            25330..26502
                     /locus_tag="PLW08_02195"
     CDS             25330..26502
                     /locus_tag="PLW08_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51444.1"
                     /translation="MIKRLFFTAIFCLLAIFLLAQADSLYLLLPQFSNPFYDTFSRNY
                     IGSTSAGRGYTGVSVLGGLDNALLNPASVLPDSAKFFIEMNIKPSLEAEGYPLYANYT
                     SPFPLGLLGLSFAMENKFSLGVLYNLPKSITLDDYSFFINQGADLIQRFPVYNLHQAT
                     ALIAFHNGPLHLGLNLHNQFHYIDDPIFLRSYERIRDSQYHLRIQPGIIYQLGSANIG
                     FSMMPATKFSWNLKCANYDFKMPLWLTGGIEFVKPEFTLATEAEWEQTSDICDSFNDR
                     FTFKAGLEKMSDKLVYRLGYHFTSNVYSGMIRLAQNVVNPDTSNAWNTVPQAVFIEDN
                     AQHSLTAGLSYRFKNGIFNLSAMQVILGDVNKTQINVSLCFYLNYIFRKREIHLNE"
     gene            26495..27619
                     /locus_tag="PLW08_02200"
     CDS             26495..27619
                     /locus_tag="PLW08_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPL51445.1"
                     /translation="MNKPAVEIRRIDSYDLPVIEEAVADFFTEIKSPKINHSKRVLIK
                     PNALGAYPPERAVTTHPIVLEAIIRYFLDRKKEIWFGDSPGNSVAFETVWQTCGFSSL
                     AEKYHLKVVNFSTLGFRELSYKDISIKVSEALFQCGIIINVGKYKTHQLTAFTGALKN
                     LFGFVPGLAKSEYHRLYPDTNSFAAMLTALYAVIGNRITYSIIDGITGMDGNGPSAGK
                     PHHFGLLFGSTSIPALDYVASRIMGFQLKDVPYLSSALIMDGILPSQIKIPVSFRNYS
                     IPDADINSVKVIQKTLKYVPGIARKAFRKVYYFYPAVSERCKQCGICVKSCPVKAISW
                     QNNTKPYIHKEQCIKCLCCHELCPYQAIDIKKSLLARLGV"
     gene            27619..27942
                     /locus_tag="PLW08_02205"
     CDS             27619..27942
                     /locus_tag="PLW08_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPL51446.1"
                     /translation="MKSKTKKLSPTLRFAFWAIIVLLIIWILFLANNSFLNTWKLKRR
                     VEQLEKETSILKAQNDSLAQENERLKTDPEAAEKAARENFGLTKPDETVFRFVPAKED
                     EPPKK"
     gene            27923..29623
                     /locus_tag="PLW08_02210"
     CDS             27923..29623
                     /locus_tag="PLW08_02210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPL51447.1"
                     /translation="MNPQKNSQPHIYNISLFTNVEQDTRNKLKKLVHDLILANEDSRL
                     LIIEKIADIVIPACSGNLDNQLFSEFSGLLIEYLLDYFLELKATQESEFYHFLSFLSH
                     FPVTEDSPYYPYVQKIMNDHKESEPELMNIFNRLQLLYLLTENGDFGNAEELLEELEP
                     VEKNKHLELWALFQLSQTRIYRHQNKPAELLETQLNLILEAYIRDSSDSAINFIIRWL
                     ISINWFKQNIIKKTLLLRIKDYLLEQKSLNTAMVLYELFSMEDRLVPSVEKIEYQRIL
                     IKYPVSMLNVQQLHTLYFFAGYYNCGVLSNFKDSIQNYQYSNYFLHKSWDSFIHLSRF
                     MREHLDPSRFYNAVPYLESRIKELSNQASLQNNAYVESLQANFYKIEELYEKVGELSL
                     TDSLTGLRNRRYLEGNLFQMVILAARHNVPVCFSMIDIDFFKLVNDNYGHLAGDFVLK
                     ELARILNSEFRKSDVVIRYGGDEFLVILFDSDQKLSYSIMEDLRQKIEAYSFDYNNQI
                     ITFTISIGIACDFHISSQPKNLAKCIACADQACYVAKTNGRNRVELYQSPTGATDNNC
                     "
     gene            29696..30799
                     /gene="ugpC"
                     /locus_tag="PLW08_02215"
     CDS             29696..30799
                     /gene="ugpC"
                     /locus_tag="PLW08_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPL51448.1"
                     /translation="MAKVTVKNLNKYFDNGVHAVKDVTFTAEDKEFVVLVGPSGCGKT
                     TILRMIAGLEEVSSGEIYIGDVLVNNVPPKERDIAMVFQNYALYPHMTVFDNMAFALK
                     MRHIPKEDIKRIVYDAAKLLGIESLLKRKPGQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRAEIVKLHHTIGNTMIYVTHDQVEAMTMADRIVVMKDGVIQQIDT
                     PLNIYNNPANMFVAGFIGSPAINMTKGKLIRKESDLYFDSEDYQLKLTPRQAEVLQNY
                     TNKDIIMGIRPEDIYDSRFDAMAESPQKFITKVDFVEPLGNEYHVILTTANNEYTARF
                     DPKDLPKMGQDLGVTFDMAKAHFFDPESEVNLF"
     gene            30847..31614
                     /locus_tag="PLW08_02220"
     CDS             30847..31614
                     /locus_tag="PLW08_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51449.1"
                     /translation="MGKQYIHILSVTFILFCSNPFLFSVTTENSASANALSGITMLSA
                     SPADYTLSPVIGESGICFSWHQPFGISEINVYGLHSAFPVKPLIVATGIDYLAHTDYR
                     WQNEYLALSASFSFFRIGATQHLIYEKIAEQSWYTWDNDFALSFQNKRFGSEIRCNNI
                     RTEERTFTLSANFNANENSSFATSYSFRPKEKDSYSLATSLSIAKPLIIQSSWQSEPA
                     RFGLGLKVLIGKVNLMYTVRTHTELDLTHIVDLGSAW"
     gene            31611..33119
                     /locus_tag="PLW08_02225"
     CDS             31611..33119
                     /locus_tag="PLW08_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51450.1"
                     /translation="MKFFGWFLVLFTFCCVPLFSQIEEIPELESETLEDNWLNQLYTE
                     LETGKHKVDLQSRTKYQETFANGLANLQFRQKDMRFNLNLYKKENSDLYANFQLSAMP
                     SKNKPSEIHFGSFRPAFGMGTILKKSGGNSLFAIKRAGHPASFSPFGTGAILRVNNFS
                     AFFMASGQKRNASLKEGKIGSLYKSVQSEKTSVQEEIIAGGLEYHYKNNNFALLAYNQ
                     NYDRDFADTLWVKKLNAYSISAKTTGENYVLSAEIARLEENIAFQSSVKFIYNYISQE
                     LSYSYRQGKQLPAYAAKPCLLSNQGENREIDWNLDYQLPKNMSLGLRYALMRKNNALK
                     SPNWNSRGIFNLTFNPSYTIINLQLTLLDKEIVTETDSSYIATLPVHYRLRFKIDHEL
                     NPKLTLSLLLRYHYEDKIERRNNSFYWENAFHYHRTKWQISAGIKTWQTLTSLILPET
                     DTENPEGCICATSEDDRVFAKVTYKGKVINLKTEWQQSWLNGNRTLYLSVGL"
     gene            complement(33533..34186)
                     /locus_tag="PLW08_02230"
     CDS             complement(33533..34186)
                     /locus_tag="PLW08_02230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL51451.1"
                     /translation="MQENKTASFFDRYSIDFNAIYGSGTGPFQRLINKWFRQTMLIRF
                     QKTIVACNPLEGKTILDVGCGPGIYSITLAKGSPNFVYGIDFAPSMVEIARQEAKKAG
                     VENICRFEIADFNTLPEDKQYNYLILMGFMDYMKNPKQVIEKTMRLASDKVLFSFPSD
                     KGFLAWQRKIRYKFKCPLYLYNEKQLENLFSDISPWHYTLENIGRDFFVTLEKGENL"
     gene            complement(34241..35113)
                     /locus_tag="PLW08_02235"
     CDS             complement(34241..35113)
                     /locus_tag="PLW08_02235"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL51452.1"
                     /translation="MREYPKSIFTIDVEDYFNVTEERGEPPVSEWDSLPSIVESGFLK
                     LLDLLDTYQVKATCFFLGYVAKKYPHLVKEAQKRGHEIASHGMFHRIVYKMSKDEFTT
                     DLLQSKNILEDICSEQVTAFRSPSFSLTEASPWFFETLAETGFIADSSVFPVKRDYGG
                     YKTTQKKPYWITTEKGDICEFPISVAPFCGKDICFFGGGYLRLFPKRVILYMHKKLAK
                     QGIPTLFYIHPREMEPQHPRLKMNKMAYFKSYVNLNTVQPKLEAILSSSKFITCRQYI
                     ETCFPGKRISSSST"
     gene            complement(35426..36424)
                     /gene="gap"
                     /locus_tag="PLW08_02240"
     CDS             complement(35426..36424)
                     /gene="gap"
                     /locus_tag="PLW08_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPL51453.1"
                     /translation="MTNVAINGFGRIGRLVLRVILDSHPELNVVAINDLTDAKTLAHL
                     FKYDSVHKIYPGNVSSEENYLVVNGKKFRIFAEKDPEVLPWKDLGVEFVIESTGIFTS
                     KEKASKHLKAGAKKVILTAPAKDEVDATIVMGVNHTSLKAEHTIVSNASCTTNCLAPV
                     AKVLQDKFGILNGLMTTIHSYTNDQRILDLPHNDLRRARAAAMSMIPTSTGAAKAIGL
                     VIPELKGKLDGVAIRVPTPDGSLVDLCANLEVEVTKEEVNMAMKEAAETYLKGYLMYT
                     EEPIVSIDIVGNSYSSIFDALITYTKGKMVKVFSWYDNEYGYSNRVVDLLEYMRKL"
     gene            complement(36446..36988)
                     /locus_tag="PLW08_02245"
     CDS             complement(36446..36988)
                     /locus_tag="PLW08_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPL51454.1"
                     /translation="MNIVYEYSHLGGLEILLVRYPKIMEEVLNIIKDIKPHKTKISKE
                     KTMSGKSLFSPKDINKQFKDKFQLLGYKELRDTFSIELPNYEPKITGCYKQIDFCLGK
                     VLVEVQLGKYPFMFYDLAKFQYFFNENKADVGIEIVPCYQLHKSMSTGVSYGEQLIND
                     IERLKRHFPAVPVAIYLIDI"
     gene            complement(36985..37917)
                     /locus_tag="PLW08_02250"
     CDS             complement(36985..37917)
                     /locus_tag="PLW08_02250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51455.1"
                     /translation="MVKISNKWNSDSILCLFQGDCLELLDSIPDAAIQLVVTSPPYNI
                     GKKYEKRQPLDEYIDWQKKVITECCRVLKKSGSICWQVGNYIENGEIIPLDILLYPVF
                     AESGLKLRNRIIWHFGHGLHSSKRFSGRYEVILWFTKSDEYIFNLDPVRIPQKYPNKK
                     YFKGDKKGELSCNPLGKNPSDVWNIPNVKANHIEKTIHPAQFPVELVERLILSMTNEN
                     DWTLDPFMGTGTTQIASLIHNRKSCGAELLDEYYEIALQRINSAISGELKIRPMNRPI
                     FDPEHPISFKPITVNLTKIKEEQSELDLVWSNEK"
     gene            complement(37923..38897)
                     /locus_tag="PLW08_02255"
     CDS             complement(37923..38897)
                     /locus_tag="PLW08_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPL51456.1"
                     /translation="MFLTGTQLKEVFLKAKKQRFGIIASNVVFDTQVRALIQGYAAVN
                     SDGLMQMSTGACKYAAGTSQDLHTGAKMISAMVKIFSAQFPKCGIGLHIDHATPNYFD
                     FIKFCIENDLVSSVMIDASAEKLEDNIRITKEVVDLAHKYNILVEGEIGHIKGTEDEI
                     VSEDELYTKPEEALEFVIKTNVDLFAASVGTNHGVTKGENVVLHLDLIKEIDDLLIKN
                     GAERGLVLHGASGLTDWQQQTAIANGVVKINKDTHYQMDMASAIQEYWEKEKYAIVCP
                     PDVDPAAYIPNKSKFDPRKWLIKGEEKMQNTVMEFCKMSGSANNSILL"
     gene            complement(39036..>41480)
                     /locus_tag="PLW08_02260"
     CDS             complement(39036..>41480)
                     /locus_tag="PLW08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPL51457.1"
                     /translation="KYVIVSGATNDEVYMWVNPVIGETEPAPQLTAADLTGTDIGAIG
                     AVAIRQGTNGPIARIDGIRVTNNWAKLWEGSPMETPVIHTSTTELDPLVSIVNLPSDE
                     TRDYTLWGTNIVGGITVTAPNGFQVSTSQIEGWASSITVPADFHSQIYVRMLANAIGE
                     YEGYIVHTSGDAEPVNVRVTGAALPTPVTWNITANLTPFSSEAGTPSAVQSYTLSAPG
                     AIDSLQITTSYPFELSSTGTGNWGISLTLSNNFSGNVYVRMNAAGSGIFNGIIYHNTT
                     YANEYQLPVSGTATSQPGMASDLFFSEYIEGSSSNKAIEIFNGTGGPVDLSNYKVCLY
                     ANGAATPSNTLNLTGTLAHLDVYVIANTSANATILGLADTTSSVTFYNGDDALGLYKL
                     GTTETLIDVIGTIGTDPGTAWNVAGVTNATAEHTLIRKPTITQGNLDWAISAGTNADD
                     SEWIVYAQDYTDNLGMHTFGNMVVMPVFNPPAGAYATPINVTISTITPGATIHYTTDG
                     SEPTEASPVYSTPIPVSSDTTIKAKGYATGYSPSAIATAFYDFPENVSTIAQLREGTL
                     GSAYRLTGEAVLTFQQANRNQKYVQDATAAIVIDDPNGKITTTYNLYDGITGITGTLS
                     SYSGLLQFVPIADPGAATSHNNTIVPVTRTLATITSADQAKLLKIYSVTLTPNATGVF
                     AATAENITATDASGTAVMRTFPNADYAGTAIPTDPVDIVCLGGQYNDTMQFSPRFLAD
                     ITPAAGILEAPIVNISQVAGMINLSWNQVPGATNYRIESADDPYGTWSTVTTTPNLFY
                     SGTAVSKKFYRVIATN"
BASE COUNT        12359 a         7994 c         8088 g        13039 t
ORIGIN      
        1 taattgctta ttttatagtc cgttcctaat agaccccgta acacttctgt aacacttcct
       61 taacacttcc gttgtctcgt tacggagtcg ttacttattt gtaacctggt tattactgca
      121 aacagagaag aagggtaaat gtatctctac aatatacttt tggcagaaat agagaatcag
      181 aatttggcat aaaggtggaa aacttttcct tgacattcct tttttgtttt ttttaagggt
      241 taagtaagat aatatgagtt agaaggaaga acacacaatg gaaataatca cagaagcact
      301 caagtttccc tctgattgta acataatctt aggtcaaagc cattttatca aaacagtgga
      361 agacctatat gaggcgcttg ttaccagtgt accggaaatt aaatttggtt tggctttttg
      421 tgaatcctcg ggtccttgtt tagttcgcaa agaaggaacg gataatgaac tgatagaaat
      481 agcggtggaa aatatgtttc gtttaggtgc tgggcacagt tttctgatta ttctgcggaa
      541 tgcttttcct atcaatataa tgcaagcggt taaggactgc cgggaagtgg ttaatatttt
      601 ttgtgctaca gctaatccgg tagaagtgat tttagctcag acagagcagg gaagaggtat
      661 tttaggcgtt attgacggtt tttcacctaa gggaattgaa ttggatacag atattacaca
      721 tcggaagaaa ttccttttgg atattggata taagcgttga aaatgatgat tcgcactttt
      781 attgccctgg aattacccaa acccttgaaa agtgaacttg ggagcgtagt taacagatat
      841 tctaaagcaa ccccacctta tgtaaattgg gttaaaccgg aaaatttaca tttaacactg
      901 ctttttattg gagatgttca gccccaagaa ataaggatta tagatgaagt gctggcaaaa
      961 caactggaag gttttccctc tttcattttt cgtcctgagg gtttggaatt ctttccttct
     1021 cttcaacccc atcttttatg gctgaagctt tccagtgaaa atgaagatat tttcaaactg
     1081 aataggcgtt tgctgaaaga gctttccgcc tgcggaattg aagcggataa aaaagcaatg
     1141 aaattacaca tcactttggc acggttgaaa tcatccctaa atcctgcttt ggaaagagaa
     1201 ataatgagca gaaatataaa acacgagccc ctatattttg actgcctttg tctgtttaaa
     1261 agtcaattat ttccagaggg accgcaatat acagttttaa acagatataa tttaattcac
     1321 agccggagga taaatgctgg ataaaaacaa agatgctgcc ttaaaaacag caatttcaca
     1381 actggaaaaa aaatacggtg taggaacttt aatgcgcctg ggagataaac ctcatcaggt
     1441 tgtagatgta attcctacag gagctttgaa cttggatatt gccttgggaa ttggaggtat
     1501 tcccaaaggt agaatcacgg aaatttacgg tgctgaagca tcaggtaaaa caactctggc
     1561 tttgcatatt gcggcagaat gtcaaaaaca gggtggaata gttgccttta ttgatgttga
     1621 acatgccctt gatcctattt atgccaagcg cttgggggta attacagatg accttttgct
     1681 ttctcaaccc gatggaggtg aacaggcact ggaaattact gaaactcttg ttcgcagttc
     1741 tgcagttgat ttaataatca ttgattccgt cgctgcactg gttcccaaac aggaaattga
     1801 aggtgaaatg ggagatagcc atgttggttt acaggcacga ctgatgagcc aggcactgcg
     1861 aaaattaact gctatcgtct ccaaaagcaa tactgccgtt atttttatca atcagacaag
     1921 aatgaaaata ggtgccggtc cctatatgaa ccctgaaacc acaaccggtg gagtagctct
     1981 aaaattctat gcttcagtgc gcttagaagt tcggcatggt gcacagatta aagatgtcgg
     2041 ttccgaatca caagttatcg gaactcgtac caaagtaaaa gtagtgaaaa ataaattcgc
     2101 tcctcccttt aaaacagtag aatttcctat tatttttggt cagggtatct ccaaactgga
     2161 tatcattctg gaaatggcag ttcagcatga tataattaag aaaagcggtt cctggttctc
     2221 ttacaatgac ctcaaactgg gtcagggagc agataaaacc aagctctatt taacggaaaa
     2281 ccagccactg ctacaagaaa ttgaagccaa actaagggaa aaaataaatc ccgaggaatt
     2341 caatttaagt gctgaagaaa atgttcctga agacaacgaa gaaagctgaa cacgataaaa
     2401 actcgctgat tattattgac aatgaagtaa gggggattct tcctgataga atcctccttt
     2461 cttatttccc tcttcctttc gccggggaaa ttgacgacga acaaggcaaa gaactacttt
     2521 cactaattgc caaaaatgcc cggcaacaac tcctaaaata tgtatccgat caagaacaca
     2581 gctctgttca atgccgacaa ttcctttccc gaaaacacta tcctgcggaa ttgattgata
     2641 atctcataaa agaatttcag aacaaaaaat atattgacga ctttcgcttt gtgcaaatcc
     2701 ttattagctc tctcattgaa cggaaaaaaa gcaaacgcgc aatagcacag aaattgaaag
     2761 aaacacggct gccaaatgaa ctgtgggaaa gaaaattaaa cgaactatac cctcttgaag
     2821 aagaaaaaga aaacctgaaa gaacaaattg taaaactgcg tttccgctat cgcgatttgc
     2881 ctctcaacaa acaaaaagaa aaggtctttg cctccctcta tcgtaaaggt tttgacttgg
     2941 atgctattca ctctgcttgg caagaatgta gtagtaaggg attcctacct tcgcaggaat
     3001 aacaaaaaac gcaggtttga caggtaggcc tgcttttgct aattaaagtg gaagagagaa
     3061 aaaatgcctc gcaatatttt ctatgtaatg ggtataaaaa ttgcttcaaa tgtaggtaaa
     3121 gatggattta ttattgtgcg ctataattta ttagttcaca gagatgttaa cctattttaa
     3181 tgtcatagtg tgtatgtgtt tcagccggat gtaatattta tgtgtactcc ggatgtgtaa
     3241 aacccgtaaa caagtatagc taaatcaagg agaaggttat gaaacgctta tttttagcaa
     3301 ttcttgccct aacgctgagc ataggaggaa ttctttccgc tcaagctttg gttaatattg
     3361 gaagtggaac tgcagttaat actacttcag gagttcccac tccctacgga acattttata
     3421 agaacttccg tcagcaatat ttaattttag catccgaact gaatgatgca ggtggaggtg
     3481 ccggtgatat cacttctatt gccttcaatg tcagtaactt aaataattgt agtccgatgc
     3541 cgaactacac gattagatta aaacacacaa ctcaaacaac tctcactact acttttgaag
     3601 taggggatta tacaaccgtt tggactgccc cggaatttat gcctacaact ggttggaatg
     3661 tgcataattt cactactcct tttaattggg atggagcttc aaatctcctt gtggatattg
     3721 ttaccacttt gatacccgga agttttaccc agaatgcttc tgttttttat tctactacta
     3781 cttttaacag ctctctccgc tttcaaagcg atagtgccga tgcttcttct tctgcaaccg
     3841 gaacaacttc cgtaaatcgt tccaatatgc aattcattat ggctcctcta atagttacca
     3901 atccacccaa tccagcaatt ttagtttctc ctgccaatgg agctactttg gttagtcctt
     3961 cggcaaattt aaattgggtt agcggtggag gtgctccttc cggttttaaa ctttctctgg
     4021 gaaccaataa tccccctaca aacatattga acaatcagaa tctgggcttg gttactacct
     4081 atgatccccc tcaagatttt gatcttaata ctacctacta ctggaaagtt actccctata
     4141 atgcaatggg agatgcggta agctgtcctg tctggagttt tactactcac ggtgcgccta
     4201 tagtaaatac gcttccctat atgcaaaact tcgatatttc agttactcct ccagctttac
     4261 cttttgactg gacaagcatt gtgcaggcat cggtaacaac cgcttatgta gatacttatg
     4321 cttccactac ttatgctcac agtcaaccaa attgtgtccg tctctataat tctacggatg
     4381 ccagtgctac ggttatgctt gtagctcctg ttttaggaga tgctcttgat ccgcattcgg
     4441 tcagaattaa attctgggct cgttccagca gtgccggtta tccgctttca gtgggagtta
     4501 tgaccagtcc aactgatcct gctacctatc aggaagtgca aaacattgct cttactgcta
     4561 cattaactga atatgtaata gatttaacta cttacactgg aaacggacgc tttatcactt
     4621 tcaaacatgg acttggtgga acaagcagaa ctttgtatct tgatgatatc agttttgaat
     4681 taattgctcc gaatgatttg gctgctcttt ctataaccgg aaatgttact cccagtgtag
     4741 gtaatgctac taactatact ataacggtta aaaactgggg aactgcatcc caaagtgcct
     4801 atatagttaa actgatgagc ggagatacag aattggcaag tgctcctgga ccaactattg
     4861 cagctggagc tactgctact gcagttgtaa cttggactcc tactgcagaa ggaccgatga
     4921 gcatctatgg aaaagtagtt cttggtagtg atgttaatcc tttaaacgac aattctccgg
     4981 taatgaatat ttccgttatg cctcctggag ccactgtaat aactattggt gagggtaatc
     5041 taaatgaagg cgttccctgg gaatttttct acaaaaacag cttatttcaa actctgtatt
     5101 atgcaaatga aataggaacg ataatgggta atataactgc ggttaccttc tacaataact
     5161 ttacttccaa tttaattgat aaaccggtta aactttggct cgggacaact gatttggcaa
     5221 atcttagttc cggttggatt ttggatgggt taacattggt ttatgacggc actttaaact
     5281 ttcccaatgg ccaaaataca attgtagttc ctctacagac accatatcta tatacaggtg
     5341 gtaatctggt tctttatgcc aacaggccta tggatactca atattatctc tccacggatg
     5401 attttaaagc tcaaactgtt ggcactaacc gtgcgcgtaa gttatattca gattccgtaa
     5461 cctacgatcc tatgagtcct tctgctgcag gaacgctttc cggaactttt cccaagacct
     5521 cattaacctt tgtagtaacc ggaatgggtt ctttaagcgg gacagttacc agcagtggtt
     5581 ctccggttgc ggatgtttta attcaagtga gcaatgccag ctattctttt acccgttata
     5641 ctaatgctgc tggtgaatat tctttccagt atttgcctat tggcaattat actgtaactg
     5701 cttccaagct gggttatgaa actcaatccg ttaccgtaac tattattgag aatcagaata
     5761 cggttcagaa cttcagtttg gtctcttcca acacggtatc tgtcagtgga catattgttg
     5821 gtagcgatca gccgactgta ggaattgcca atgctcatat atttttggat ggacctctgg
     5881 attatgaagg catttccaat gccaatggtg attttacgat aaccggtgtt ctttccggaa
     5941 atacctataa ctatactatt caggcgattg gctatgcaga tttgaccgga actgtggtag
     6001 ttggttccac taatgtaaat atgggaacct tggtaatgag tgaattagct cttcctccgg
     6061 tgcaaattgt agcagaagaa aatgctgctc aaacgcaagt tacccttacc tggcgtcctc
     6121 ctggctcaac aggaaccgga gttggtacgg aagattttga aattgacgat ggcggttggg
     6181 tttctagtgg ctacggtgac tggcaatggg gtcattataa tgtggctaat tatgtggata
     6241 tagatactta tacagatact cctcctcaat ctgcacattc cggaaccggg atgtggggaa
     6301 cggtattgga gggaggttat tccaattgca atacctggtc ttatttaaga aaaaccttta
     6361 atttacaaag tgtttctaat cccgtattga gcttttggca ctatatgaac ggttacaata
     6421 cctgggatta tggtttaatc aaagtaaacg gcaatactgt ttggggcaac agttcttcgg
     6481 ctgtctttat gccatggcag gaacttacta taaatctttc tgcctatgct aacctgagtg
     6541 aagtggaaat aagttttgag tggtttgcta caggcactgt ttcctatgcc ggatggtata
     6601 ttgatgacct ctatgtaggt ccctctatga acaaaaccgt aaattatact tatgtgccta
     6661 ttcccgaaaa agcacatttg ctttctgaag aggaagaagc attattgcaa cctaaagatt
     6721 ttccgccagt taatcgctca ttttctttcc ccaattctca aatatcaaat ccgaacagag
     6781 cattaaatgg ttacaaggtt tggcggttta ttttcggtaa cgaaaataat gagaattcct
     6841 ggactttgct tactgctaat actattaccg atacaacctt tattgatact gcctggggaa
     6901 ctttaccgga cggaaattac cgttgggcag tgaaaggtgt ttatactaat aacttacttg
     6961 gtcctgcggg cttttccaac cggatactta tcctgagaaa tgacctggca gccaatacaa
     7021 ttaccggcaa tacaacacct tcagttggaa cttcttttaa ctatacagta acaattgaaa
     7081 atatgggaac agaaaccaaa ccggcaggca gctatacagt taaacttatg agtggagata
     7141 cggaactggc aagtgtttta ggacctacta ttgctgcagg tgaagaactt gctgttacta
     7201 ttccctttac tcctactaca gaaggcacaa tgagcttaac cggaaaagta gttcttcctg
     7261 ccgacaccaa tcccagtaat aatattacac ccccaataaa tttaacagtt atgcccgcgg
     7321 gaattttagc agtaactatt ggagagggtg gacagttagt aggtattccc tgggaattct
     7381 actataagaa cagtttgttt caaaccctct attattctaa tgaaatcggt ttattcggaa
     7441 atataactgc tatttctttc tataataact ttgtttctaa tttaactgat aaaccggtta
     7501 aactgtggtt aggaaccaca aatctgcagg atctcagtgg cggttggatt ttggacggtt
     7561 taactcttgt atatgatgga accttaaact tccccagcgg tcaaaatact attacagttt
     7621 ccctgcagac accttatctc tatacaggtg gaaatttggt tctctatgct aatagaccta
     7681 tggataccca atattataat actaatgatg attttcaagc acagacaata ggttccaacc
     7741 gtgctcttaa aatccaatcc gattccgtta cttacgatcc aatgaatcct tctgttgcag
     7801 gcacactatc gggtcaattt cctaaaacaa cactcttttt ctcctctccg ggaccaaatc
     7861 ctatctttag tgtaggtcct tcttctcata actggggaac agtgttaatt aataccgtga
     7921 acgagcagaa ctttgttgtt ggcaatgctg gaggtggaac tcttacaatc aatagtatta
     7981 caatcagtgg cagtgaaatg ttctctttgc agaatttacc tgctctgcca gccaatcttg
     8041 cattttgcga aaccattaat ttcacagcca gatacaatcc tactgctgtt gggaatcata
     8101 cggctactat tacgattacg gataatctgg caacaactta cactataacc ctcaacaaaa
     8161 gtaataatga tggggataaa cggatttctc ataatgtttc cctttccgga aattgtattg
     8221 ataccacatt gaatatttta ccttacgccc aaaactttga tcaggttacg gttcctgctt
     8281 tacctctgga ctggatgaaa attgtgcaat caacttccac ctcagcagta gtggaaactt
     8341 atgcttctac tacttatgct cacagtcagc ccaattgtgt tcgcttgtat aatccttctg
     8401 atgccaatgc cactttgatg ttgattgctc cacccttggg tacggcaatt cctacgaata
     8461 caaccagggt aaaattctgg gctcgcagtt ccagtgctgg ttatccaatc tctataggaa
     8521 taatagtaaa tcctaccgat ccttccactt atcaggaaac tgaactgata tccttaacca
     8581 ccaccctcac agaatatgta gttgccttca atgcatacac gggaacagga aaacatatcg
     8641 tcttcaaaca tggtttgggc ggaaccagtc gcttacttta tgtagatgat gttatgatag
     8701 aaattattcc gaccaatgat cttgctgcaa cagctatctc cggtaatgta actccctccg
     8761 taggacaaga atcgcagtat aatatttcca tccataattg gggaacggct tcccaaaata
     8821 cctattcggt taagctgttc tctgctactg gtgctgaact tgctacagca gcaggaataa
     8881 cttgtgcgcc tggagctaca gtagatgttc cggttaactg gactcccaca actgaagggg
     8941 caatgactat ttacggcaaa gttgtcttgg ttggagacca aaatagtttg aatgaccaaa
     9001 cacccaactt aaatattctg gtaaatcctt ccggggtctt tatgattacc ataggaacgg
     9061 gaggtcagtt tgctcggatg cctgttgata tgtattatag aaacagcatc tatgaagggc
     9121 tatattatcc tgccgaaatg agtaacttta tgggtcagat aaccggtatt cagttctata
     9181 acaacttcta taccgatctg cccaatatgc ctactaaggt ctggatcgga actaccactt
     9241 taacttcttt gtcggatgcc tggattccag tttcagagtt aacccttgtt tttgacggga
     9301 atatcaacta tcctgctggc gataatataa ttactattcc cttcaatgct ccctatctct
     9361 atctgaatgg agaaaacctg gtaatctttg tgcagcgtcc ttttgaagat gtatatcaca
     9421 attccagcga tgatttccac gcacaaacgg atgccactta tacagggcgc tccagacgaa
     9481 tgtatagtga ttctgttact tatgatccca ataatttgcc tgctacagga gcaactgttt
     9541 ccggtcaatt ccccaagacc acactaattg ttattcctgg aggagtggga catttggagg
     9601 gcacggtagt tgctactggc agtattcctt tggaaggtgt agcggttaat attgccacta
     9661 ccacttacgg cgcagttaca gacgctcagg gacattatca tattaccaat attattcctg
     9721 atgattatac tgtgaacttc agcaagtatg gttacatcag ccaaagtatc aatattacca
     9781 tagaggagga tgagaccgaa attctgaatg taacaatgca accgatgcct accgtttctg
     9841 ttaccggaac tattgtagcc agtgatacag gagctggaat taacggtgct tccatctatc
     9901 tggttggtta ccaaaattat aatacaacta gttctgctac tggtgctttc agttttcctg
     9961 cggtttatgc caatcagacc tatgaatata caattattgc tactggttat acaactgtta
    10021 cgggaacaat caatgtagga gccactaact acaatatggg cagtatcact atgaacgaag
    10081 ttgcttatgc accttttggt gttgtagcag ctctgaacga tatttataca gcagtgaata
    10141 tcagttggca ggcacctgat cctacagcag tagatgtaac tgagggtttt gaaggaactg
    10201 tctttccgcc gttacaatgg actcaaatca tcaataatac aggaccggaa attattcctg
    10261 gggttttacc aacctggtgc agatttggaa ctgtgaatcc tggaggaaat cctgttgctc
    10321 caccgcaagg aaattatcaa gcaggtctgt ggtgggatta tgaacatcag gatgaatggt
    10381 tgattactcc tactttcaat tgtcctccaa tggcttatct tactgtagat gcctatgttt
    10441 atcggggttc tatttataac gaccactatt acatcaaggt ttctaccgat aatggcacaa
    10501 attggactat tttgtatgat gctacagccg ctacaggtgg ctggaattat tatgtctcac
    10561 ctatctatct gaatatggaa gcatacgggg gacagcagat taaaattgct tttcaagccg
    10621 atgatccccc ttccaatgat ggcctctggt attgttggtt tatagataat ctctacattg
    10681 gcaatgcggt tcaaggtgtg aaatttgcgg gttccgacct gcttccttct cgttctaaag
    10741 ttgcgggaga gaaggtctct tctgagttaa ttgttgctcc ctatccttcc cgagaagcaa
    10801 ttgaagggaa gagagagctt cccacagttt tgaaagacaa aatgacaact ccaacgcggt
    10861 cggaaaggtc tttgcagggc tataaggtct ggcgtttaac tagcggaaat gagacccaac
    10921 cggatacctg gactctgcta actactcaat tgataactac tttggcaaca gttgattctg
    10981 cctggactgc acttcctaat ggaagttacc gttgggctgt gaaagctgtt tatactgcca
    11041 atgttacttc tgttgcttca ttctccaatg ttctgaataa agaaacacag atgggtaata
    11101 ttgttggttt tgtgcggaaa caaaataatc agcccattgc cggagccaca gtaactgccg
    11161 ggggaatttc tgctaccact aattctgcgg gagcttattc tttggcttta tccataggaa
    11221 cttacagtgt aactgcttca gcagcgggtt atcaaccgcg aacaattgat ggggttgtgg
    11281 ttttgcccaa tcaaaatacc accttgaact ttgtgcttga tccggtagct aatgaggatg
    11341 aatatttgcc tgctgtttct acggaactga tagggaacta tcccaatccc ttcaatccgg
    11401 agaccactat tgcctattct attaaggata gatgcaaagt gcatttggag gtctataacc
    11461 tgaaagggca actggtgaga accttagtga atgaagaaaa ggcaaatgga cattataaag
    11521 cggtctttaa cgctaaggat gagaaaggta ataccctttc ttccggtatc tatttctatc
    11581 gcttgcaagc ggggaattat gtttctacgc ggaagatgct gctgatggaa tagacagagt
    11641 tgaagggtta gacactaatc aatatatatc aagggcggaa gttaattctg ccctttttgt
    11701 gttttatatt ggattcctgc ttgtgcagaa atgacaaaga tagcattctt gcctatgatg
    11761 ttagcgtttg tcatttcgct cttgatgcgg aatcctatta aatattgttt cagaaagaac
    11821 ttcattttcc acttgactgt aaacactaat caggatacat tgtttttaga attttattaa
    11881 ttacgggttt tcccgcaggt tttttgtctt tcgcaagtat aaaataaaag taaatctaat
    11941 ttcagaagaa ggagatttaa tgatgaaaat gtggaaacaa atagtcctgg tgatagtggc
    12001 attattctta atgagttgtg ccatagcaaa agataatatg attgacccta atgcgcccaa
    12061 tcctgtagta gaagtagtta aaaatgttcg cgatgcggta gttcagatta gggtagaagc
    12121 aaaagtgacg gttcagaatt atattaatcc cttttttaac gatccctggt tccgtcaatt
    12181 tttcaatttt cctcaggaac agacgcgtcc tattgtttct ttaggaagtg gttttattta
    12241 tgaatataac cctaataccc gtgaagcatt tattttaacc aataaccatg tagtagaaaa
    12301 gggcagggaa ggcacaatta cagttacgat ggcggataaa gttgtctata ctgcaacagt
    12361 agttggttta gaccccaata ccgatgttgc tgttattaaa attgtagtta aagaaggtga
    12421 aaaagtaact gttgcaccgt taggtgattc ggacaaactg caaattggcg aatgggctat
    12481 tgctattggt aatcctttca gcgaaggact ggatagaaca gtaactttgg gagtgatttc
    12541 cgcaaccagc agagccaatc ttaatttggg tgataatgct cctatctatc aggattttat
    12601 tcaaaccgat gctgctatca acagtggcaa tagtggcggt cctttactaa atatcaaagg
    12661 agaagttata ggtatcaatg ctgctttggc aagcaccaac ggaggcaatg taggaatcgg
    12721 ttttgctata cctatcaatc ttgccaaacg ggtggtagaa gaccttgtag ccagtggaaa
    12781 agtaacccgg gcttatattg gtattttggc tcaggaaata acccaggata ttatggaatc
    12841 ctacggtttg aaagaagttg caggagttct tgttgccaaa gtggaaaaag attcacctgc
    12901 cgaaaaagca ggtctccaag ttgaggatat tattctggaa ataaacggtc agaaaattac
    12961 cagcgttcca aaattccgta tagcgatcgc tactgccaaa gcaggacagg aagtagcatt
    13021 aaaaatcttg cgcaataaaa aggaaatgac cgtgaaagta atcctggaag catatcctga
    13081 agatactatc gctacagcag atacagccaa aggaaccctt gctacaggaa tcactgtaga
    13141 agcaatagat agtcctacag caaagcgctt gaatgtgaaa agcgataaag gtgttgtagt
    13201 aactaaagtg gaaccgaatt ccgttgctgc caaatctgga ttaaaagttg gctatgtaat
    13261 tctaaaaatt gaaggcaaag aagtgaacag ccccaaagaa ttcaatacag aactggaaaa
    13321 agcaaagacc aatatggaaa aggaaaacag gaagaccatt cgcttgtatg tgctggatac
    13381 taacaatcaa ccttccattc ttattctaaa atttgaataa gctaaacaga gagaaacaga
    13441 ttaaagcgga agataatatt gtcttccgct ttttcacttc cccggctaat atcatatccg
    13501 tttgggaaat tcctttgtta ttcctgtaca ggcaggaatc cagagagttt taatctatat
    13561 aactttttct gcttgacaaa agaaattgga gaaattaaaa agagaatatg aaaaaactta
    13621 attctgctaa ctacaaagat gtaaatttct gtctccggtg cgggcataaa cttgagctga
    13681 aaacagacca cgaaaataag ttgagacctc attgtcctgc ttgtaattgg gtttattata
    13741 aaaatcctat tccagcagta gcaattgtgc tcttcaatga aaacagggaa ttacttttag
    13801 tgaaacgagg tctgcaaccc aaagcaggtt tttgggcttt gcccagtggt tatatggaaa
    13861 ttaacctcac tccggaagaa aatgcccttc aggaactgga ggaagaaaca ggtcttaaag
    13921 gcaaaattat gcattgcgtg ggttggtttt ttggcaaaag tccaatctat gaaagagttc
    13981 tcagtatcgg attcaggatg aaagctattg ggggaaaact tcaagccgga gatgatgctg
    14041 ttgatgtaaa attctttccc ttgaataatc tgcctgttat tgcctttgat gcccacaggg
    14101 attttattgc caaagaaaca aagctataag tcaagaggtc gagaagtcaa ttaaacaaat
    14161 ttgagtgcgt gccaattcta actaaaatca gaacttcatc ctgaattcta tacagtagta
    14221 gaacatctgg ggtcaaatga cattctctgc aattattcag atttcctttt agctggtgat
    14281 ctttatattt tgccggtaag ggaatttctg ttatgagttt atttaatgcc tcttgcaatg
    14341 cgtcaataac tgttttatta tgctcatatc tcttataatc ttttttaaat tgagatgtat
    14401 agcttaattt aagcaaatca attctctaaa ttctttagca gttcatcaat agtattaaaa
    14461 acgggtaagt tatctggatg ttttgattct tcaatagctg tcagcgtttt tttattgggt
    14521 tggggctgtt tacttacagt aaagagcttt aagaagattt cctgattata agaaaagaaa
    14581 ccggaaaatc tgcaggaact attagtataa tctaaataac tgatttggtt agcagtatta
    14641 gtagtagtgc caaaacttat agatttattt aacatatctc catccctcaa aagtaagtgt
    14701 gttatgaata gtgatatttg tctcattgca ttgtttggta agtgtacctt cagaaataaa
    14761 cagatgctta ccaccttcaa tactctgaat aagagctctt ttacaattat aatctaccat
    14821 atctttatta ttcaataact ttttagggtc aaagggaata ttaagctcca gttgacgaga
    14881 aatcgtttca aatatggaat tgattaattc cgcattgcgt ttatcagctt cttcaacttg
    14941 taaccctaag tcctttgcat cttcaatgtt gattgtataa tcgtggctac cggaatcagt
    15001 gcacaggaaa tttacaatct tttctttgtt accatttggt atatggaaat ccaaaagttt
    15061 attagccagc atctttattt gtgctctggc tctatacacc tctcctaaaa ccaaaggatg
    15121 aattttttcc gaaagcacct taacaacttc agccaaggcc tgatcatttt taattccaaa
    15181 ttccttcttt actaattcta tatagccatt aattgcttct acactaacgg gaatagtatg
    15241 ccctggcata ttcggaatgg caggatttaa tggagtatta atattcgggt caataggtcc
    15301 taaagttgat tgtttagtca taacaataaa atttgaacct aagcacatta aggttcctgc
    15361 actatgcgct ttagagggaa ttataatttc cagctcatta caatattgtc ggagaaggtt
    15421 taccaaactc caagctgcaa gtgtatttcc acctcttgta tagaggaata aagatagctt
    15481 tggtagcttg gagctcttag acattctttc caaatggtgg gcaaaaaagc tatgtacttc
    15541 ctgatgaatt tgggtttccc atccaggtct atcacctgta acataaacaa gaattttagt
    15601 tttgcgcaat ttttctaagc ttttaagtag gttttcccgg cttgattttg ctctcatttt
    15661 tcttatcctt acccgttatt tcatattttt actttataat tatatatcat ttatgtcaag
    15721 ttaaaattac tccccttcag gttatttttt tagtagcgca atttctttac tcatagtccc
    15781 atatctcata gatagcatta aattcatatt aagaagaata atcttacttc agtgcactat
    15841 ttgtagtaat aaagggtttg gtttattcct gcagcagctt cggaaagggt gaaataccct
    15901 ttgtttttgg catcccaaca aactgcttca ccttgaggtt cagtaatata gggcatagtt
    15961 ttggcttctt tgctgaggat aatgatgttt ttatcatcca gcattatttt gtatctgtaa
    16021 atgcccgtat aggttttaac cagcagcagt tttccattat tactcaggtc tgcagctgtg
    16081 acccaggaaa gaggtaaaga agcaattcgc ctggcattgt ttttatcctt gaaattgaaa
    16141 ggatagggaa cgcggtaaag tcccacctgt tcctctcttt tactgatgat ataaatatct
    16201 cctgttttgg gttccagaaa gattgcttcc gcatcgcgag gtccatcttc gtaacttatt
    16261 tctatagtat caactgcgct aatttgcagg atttcatttt tgcctgtaag ctgtggctct
    16321 ttaaagcggt aaacataaac agtattatat ttggcgttgt tatcaccaat atcggcaata
    16381 taaatatagc tgatgtcatt ttgggggtct ttggcaacga caatatcttc ccaatcacgg
    16441 tttttagtgc cttttagaat tagggtagaa agcagtttgc catctatagt gagagcataa
    16501 atttcagctt tatttcctga atcgttatgg ctaaatagca cctgtttatt aagtatgcca
    16561 aaatcaatgc ctgatgcttc gttcagaaca tcggtttgta acacaaattc tttgggtggt
    16621 ctatttgcta aagcaaaaag agcaatagca gttgccagaa tgagaaagat tatcagcttt
    16681 ttgagcattt atacctctga aaggttgatg ttttcatcaa ccattttgta tgtgaacgac
    16741 aagaatgtcg ttcttccgaa aaaaaggggg aagatttata agccggattc tgtgctttca
    16801 gtttcccgaa agtgatgttc atccatcttg gctgtttttc acaaaacagc tccagctgcc
    16861 cacccgaaag cgatgcgggc aacatcatag ctttcctact tggcattgca ccggataagg
    16921 cattcctgtc tatctttgtt accaaagata ccggtggtct cttacaccac catttcaccc
    16981 ttaccatttc tgaacaacaa aaatggcggt ttatttctgt ggagcttgct ctgcattact
    17041 gcagcttcct gttaggaagt atcctgccct atggtgtccg gactttcctc tgccttttaa
    17101 atgcagcgaa catccaatct tccctctata aattataatt caaaactgct aaaaaccaaa
    17161 tttccccgta aacaatttat tgttccacat agtcattatc attcctggga aggcaggaat
    17221 ccagataaaa tgtagcacaa ttgctatgtt ttcagaacaa caattataat agaacttcag
    17281 attagttaga ggcgtcttcc gaattatcct caaaacggtt catcagaatg cgaccgcaat
    17341 tttcacaata gataattttg cgtcgcaatt gaagttcaat tctcatttgt tgacgaatca
    17401 caaaaccgca tgcaccacaa gaaccattgc gattgtaagc aactgcctgg ttgtttttat
    17461 gtttaatcat atttccgtaa tgtttaacca caggaacggg taaggtgcgt gccaggtcat
    17521 ttcttttatt gcgaacatct tcaatcttgg tttccagaga agcaatttgt tctcttaaat
    17581 cggtttcttt ttcttttttt ctggtttcgg ctttttccag ttcagctttg gcagaggcga
    17641 tcttttcttt aatttcgttt tcctggtcca tcagttccaa aaggttggat tccagatcgg
    17701 agattttatc tttgatatag gcaatttcgc tgtttaatgc cttatattct ttgttgtttt
    17761 tgatttctgc cagttgattg gtatattttt tgtgttgttc ctgcagttgt tttatgtcca
    17821 tttccagggt gcgttgtttt ttgttaattt ctgccctttg ggtttccgcc tcaagaaggt
    17881 tggcggttgc ttgttctacg ctttcaataa tttcgttaag ttgtttgggc agttcttttt
    17941 gtaaaaccct atagcggcca atttcatcat ccagggtttg catttgggct aaagttctca
    18001 gttgttcttc catagtttac tccttaaata acaaactaat atccagtgac ttataagaat
    18061 gagtgagggc tccggaagaa ataaattgca ctcctgtttc ggcaaaagaa cgaatgtttt
    18121 cttctgtaat gcctcctgaa acctcaaatt ccagaatatc tttatatttt tttactgcct
    18181 ttcttatttg cgcaggagtc atattatcca gcatcactct atccacttta gctttagccg
    18241 cttcgtcaac ttcttttaga tttgtaactt ccacttcaat tttataactg gtattatgtt
    18301 tacggatgcg atttactgct tccgtgatag aaccacaggc acgaatatga ttttctttta
    18361 tcaacaccat atcaaataag ccaaaacggt gattatagcc acctcctacg cggacagcat
    18421 atttttccag ggaacgcaaa agaggagttg ttttacgggt atccaaaagt tttacatttg
    18481 ttccttccag caaggaaacg aattttgccg ttttagtggc aattccggaa agccgttgca
    18541 gaaagtttaa ggcagttctt tcaccttgta aaatggaaga aggacgccct tccaggcgca
    18601 taatttcttc attgggcata attttatcgc catctttgtt atagagaacg attttcagtt
    18661 ccggatcaac catccgaaaa acgaggcgcg ctacatctac tcctgccaag acaccttctt
    18721 ctttcggaat aatgtaggca atattatttt ggggttccaa atccaaatag cgggtagtta
    18781 tatctccact tccgatatct tcttccagac ctttacggat tatttcatca ataaacacgc
    18841 tacctccaag gcaaaaaaaa agcatctgcg ttttgcaaat gctttttttc tattgtatgt
    18901 tcaatcttgt caggttggca taatttcttg ttttggtaac acatataact atcttcctct
    18961 tatttattca cttctatttt gtttccttgc aatctgtcaa gacaaaatat tcttttttct
    19021 cttacttttt tggcttgacg aaaatctgcc tcagggattt taggatttta aatgtaaatc
    19081 agggcaggga gttccgactt gccaaagctg ataaaatcag ttattataag tatagatttg
    19141 gtattttctg aaacggaatt aggcaagccg gcaaaatgta ataaaaaaac ttagggggcc
    19201 tatagctcag ctggtagagc taccggctca taaccggttg gtcgggggtt cgaatccctc
    19261 tgggcccacc attttttctc ccgtttttta taagtatgaa cagacaagag gaaggttaaa
    19321 tgaaaagcca ggttctggtt atctgcttgt tgctgataac tatacccctt actgctacaa
    19381 aatatgcggg agaaattttc gctgtaagtc ctggagttct ttctacagca atgggttcta
    19441 cagggctcac ttatgtcggc agtttatctg caggttggtg gaatccggct cttttagctt
    19501 tgaatcctga aagtggaata gagcttatgc gctgtgagca ttttgagggt ttaatggcgc
    19561 aaaatcaact aagtttaatt attggctcca aaaccaaaac ctcttttact ttcaatcact
    19621 tagccataga taaaataaaa ttaaccaaac tggaaaaccc cgatgacagt ttaagtaatg
    19681 aaaaccgtcc tgtcgtttgg aaaaccatta gcaatcagga ctttattctt tatgcaggtt
    19741 tggcaagaca gcttaagaaa acactctatt tgggaatttc acccaagctg gcatacaggt
    19801 ctttggcaga gaacagcggt tatgcttttg gagctgatct gggaatgttt tggcagataa
    19861 ataaacaggc aaatttagga gtaaacctca gagatttttt cagcactcaa gttctttggg
    19921 aagggggaga gaatgaaact gttattccca atctggattt ggaattgagc tatgattttt
    19981 ctcctataaa gaatattcct gttcatcttg ccgtgcggag tgaaatttta ctggaaaaca
    20041 gagaagcaac agtggaaact ggtgatttaa gcgctgattt ccacgccgga attgcagttc
    20101 agccaatttc caatttgtgt ttacttgctg gatacgatat tgattgtttc actgccggaa
    20161 tcggggtttc ttataagcgc ttagggttaa attatgcctt ccgtaatggt tcagaggacg
    20221 atttgggcta ttctcaaagg atttctgttt cttatcaatg gtaaagaaat caattgctct
    20281 tttaggagca acaggttcaa ttggaacctc agctttggca gtggctgaag agcagaatgt
    20341 ttcaattgtt ttggctacgg ctcagaaaaa ttattcttcc cttctgcaaa aagcttatca
    20401 acatcatatt ccctgtctga tttttacagg aatagaagat aaggaactgc aaaccaagct
    20461 gaaaagtgaa aatcctgaca aaaggatata ttttggtgaa gcagaattgc taaaggcaat
    20521 tgcgaatgag aattacgata ttgctttaaa tgcaatagcc ggttcggcag gactccgcta
    20581 tagttatgct atcttacaaa acaataaaac actggctttg gcaaataaag aatccctaat
    20641 tatgggagga catattttaa ccaaaatgct tgcgggcaaa ccaattttgc ctgtagatag
    20701 cgaacttagc gctctttttc aggcaattgg aaatcatcct gcagaggaaa tcagattttt
    20761 gcatcttact gcttccggtg gtattttccg cgacctgcct tttgaggatt ttaaccaaat
    20821 atcacctcag caggcactaa aaaatccgaa ctggacaatg ggagctaaag ttactctgga
    20881 ttcggcaacg atgttcaata aagcactgga agcgatggaa acgcattggt tgttcaatca
    20941 gccctattca aaaatcaagg cagtaatcca tccgcaatca attattcatt ctctggtaga
    21001 atttattgac ggttcttttt tagctcaaat gagcaagccg gatatgaaat tgccgatttt
    21061 gtatgctctt tcctatcctc aaagggttga atcgcaactt gtgaaaacgg atttaatgtc
    21121 tcttccacct ttaacctttc aggaaattga cccgcaacgc tatcccttat tttatttagg
    21181 tttggaagca gctaaagcgg gaggaattta tcccaccgtt atcaattccg ccgtggaagc
    21241 ggtttctgtc ctctttttgc agaataaaat tccctatctg aaaatgtttg atttggttaa
    21301 aaaggcattg gatgaacagg aacctatccc tgatcccgat ctggaaacca ttctgcaaat
    21361 caatgctcag acctatcaaa aagtcctgaa atatttaata taattttcat tttaagaagg
    21421 catatataaa aaatcccttt aggggttgat ggaaaaaata taatttcttt agaaaagagt
    21481 gccgtaggaa cgacagatac taacaacggg ttttaacccg gtgaaaaaga tagctacgaa
    21541 agaaataagg aaaagggaaa aacacagggc ttacattatt gaggggctta cgccaccatc
    21601 gctattattg tgtcgccctt tgggctttca aacctattgc attaattgga ttaaaaccaa
    21661 tttttatata cctattgttc tattgtcatt tctgggaaag cagcaatcca gagggaaaaa
    21721 ttataactat atatcttcag aggattgaaa tccgttatta tatatctgtt atctctaacg
    21781 ggattttata tatggctttt ccaagtggaa attatctcct ccaactttct ccttacaatt
    21841 cacccaccca caacccatcc tttactatgg gttgaggatg agtggaagtt gggttacaag
    21901 tatgcaaccg gtaacaaaca gagaaggtaa gggaaaatta gttgttattg cacgggaata
    21961 atcctttcca caatgggtgc caggttaaag gcaattctta aaattccgtt gatataggta
    22021 attttagggt tatccttatc aatgttcaaa tccggaaaga atatcttccg gtcaaacctt
    22081 ccggtttcaa tctccatatt atagtagcaa acggttgtat tggaattggg gaaaccccta
    22141 tttccgctaa ttctgaggta ttcaggagaa atggaaacgc ttatttcatc cttttctact
    22201 ccagccagtt cggcaatcac aatccactct gtatctgttt gaaagacatc acatttggga
    22261 tgccaaacat cgtctatggc attttcaatg gctaagggac tattagttaa ggcagaaacc
    22321 tctcccagca atttaagcat ttctcgttga attccaacga tgttggaaaa tattttttca
    22381 cgcatagcaa aactccgttt ttgtttaata ctcgctgcgt tctctaaaag tccatactat
    22441 ttctttatcg ggagcgatat caatgctgaa agtaacttta tcgttattat ctatagtata
    22501 tttataatcg gcttccacga tgcgggtatt aggagaaagt tggtgggtta ctttaatggt
    22561 tttgttttta ttagaattat tccttaaagt gatttggatg agacgttcgg tgatatattt
    22621 ggagatttgt gtttgttctt tcaaacgggt agaggcaatt aggtcaaaag cagttccggt
    22681 attgatagag acctcttcat ttctggaagt gtggttaatg ctgtcctcac ctataaattc
    22741 caggttgcca tcgctatctt gtttatatac cttgataatc cctttgggaa gaggcattcc
    22801 agcaccgttt tctgcagtat ttttgaattt gatgatactt ttaacattag cggcataggt
    22861 gggatattga tattcagcat aagctttaat gtttttggga ggataaagtt ccagttgttt
    22921 tgtctgatta ttggcaaagg aaaccttttg gtctaaagta tacatatgaa agtcgtgaaa
    22981 tgccttttct tcaaaggaag gagcggcaga ttctggtgcc atacctgcat cataactgat
    23041 taaactgcct ctttttccat aatagccgga ataaacccgg ttgatgtctc ccgcaataag
    23101 ctttagcatt acatcgttaa atgctttgcc ggaagtgtta ttgatggtta cccaggaatt
    23161 aaattgtaag gttttttcgt cccaaaccgt gttataagta acatcccagc ttaagccacc
    23221 cgttaaataa gacatttgaa caggatattt tgctttttta gaggcaatta tgctccaatg
    23281 caaggttggt tttgtgtaaa aattatctgg caattcagcc agctgaatcc actgagtttc
    23341 gctttcggaa attaccaata agcgctcagt tcctttttca ataataccta tgctgttgtc
    23401 actataaaac ttgagaatac cggttaatct ggattggtct ttcgtgataa tcagaacctc
    23461 cctatccaga tatttagcca taatctgcca ttttccagct aaatcgtatt caaaattctg
    23521 ctccgctatg cgaattccat ttccagtaac aattacagaa gcagggtcta ttctactggt
    23581 tatatcgcta aaattgatat cctggcgtcc attttccagc tccagctcaa aacggctgcg
    23641 aactaaagaa agatcattat tgtaaattgt taaccagtcc tctgccaata aaggtaaggc
    23701 aaacagcaga aggataatga ctgtgagaaa gttcttcatt atatctcctt gtaaaatttg
    23761 aatcctatat aacaagctaa tttttttacc ttttatgtca aggagaaatg aagggtgaaa
    23821 aatgaaagat gaggttgaaa attgtggttc ttccggtatc tgaaccacaa ctgacttgcc
    23881 tgagcactat ttcctgtcgt tatttctaat ccatcacgat cttatgcgtg ggtttcacct
    23941 tttcactttt tcaccattca cctgttcaat cgctaacctg aagaaataga ttgacagaaa
    24001 tagaaataat gattaagatg tattatataa tataaggaga agataatggc atcaatattg
    24061 gttttgggaa gtatgtgggg tgatgaagga aaagctaaaa tagttgatta cttagcttct
    24121 tcagcagatt atgttgtgcg ttttcaaggt ggaagtaatg ccggtcatac tattgtaagt
    24181 ggtggaaaaa aatatgtgtt acatactatt ccgtcaggaa tcttatatcc ccaaaccaaa
    24241 tgccttattg gagcaggtgt tttaattgat ccaccggcag ttattaagga aataaaaaca
    24301 ctggaacaag cgggaattaa ctttaaaaat cgtcttctta ttgaccttcg ggctggaatc
    24361 gttttgccac ttcataaaca aatagacctg gaaattgaaa ctaaacgcag aggaatgcaa
    24421 attggcacaa cccttaaagg aattggtcct gcctacagtg atcttactgc cagatatgga
    24481 attagaatgg aagacctggc aaaccccatt actttaaaat atcgtattaa gcaattatat
    24541 ggttaccatc atattggaat aggtaaacaa gacttggata tcctggtgga aaaactgcat
    24601 aaagattgga aatacttgaa aaaatatgtt ggcgatgtgg aaatcttgct taataatatt
    24661 gccgttaaag aggaaaaaac aattctcttt gaaggagctc agggaacttt actggattta
    24721 acttttggca cttatcctta tgtaacttcc tccagagtaa tgactgatgg cgttggaatt
    24781 ggaacaggat tttcagccag gcggttgaat aaggttttag gtgtttataa ggcatacgct
    24841 accagagtag gaataggtcc ttttcccaca gaaatgattt atgaagaaga agttgattat
    24901 attcgggaga taggaaatga atatggttct acaacaggaa gaccccgaag aattggctgg
    24961 tttgatgctg tagcaggaaa attttctgcg cggataaatg ctttggataa tgcagttctc
    25021 acttgcctgg atgttctaac cggcatagaa gaattaaaaa tctgcaccgg ttacaaatac
    25081 aaagggaaaa ttcactgttc tctggatttg aatgaattac aattaattct ttccgaacct
    25141 gtatatgaga catttgacgg ctgggataca gacatcagca actgcaaaaa tatcaataag
    25201 ttgcctttga cagcccgact ttacattaaa gccatagaag atcatattca aactcctatt
    25261 tcgcttgtat cggtaggtaa agaaaggcat caaacaattg aaattaaacc cctgtagagg
    25321 taaggaaaga tgattaaacg cttgtttttt actgctattt tttgtctgct ggctattttt
    25381 ttgctggctc aagctgattc cttatatctt ttgcttcctc aattcagcaa tcctttttat
    25441 gatactttca gtcggaatta tatcggttca acttctgcag gaagaggtta tacgggtgtt
    25501 tcagttttag gtggtttaga taatgctctt ttaaatcctg cttccgtttt acccgattca
    25561 gctaagtttt tcattgaaat gaatattaaa ccatccttgg aggcagaggg ttatcccctt
    25621 tatgctaatt acacttctcc ttttcctttg ggacttttgg gtttaagttt tgccatggag
    25681 aacaaatttt cccttggtgt gctttacaat ttgcctaaat ctattaccct tgatgattat
    25741 tcctttttta tcaatcaggg agcagattta attcagcgtt ttcccgtgta taatcttcat
    25801 caggcaactg ctcttattgc ttttcataac ggacctctgc atttgggttt gaatttgcac
    25861 aatcaattcc attatataga tgaccccata tttttaagaa gttatgaacg cataagggat
    25921 tcccaatatc atttacgcat tcagcccgga attatatatc agcttggttc tgctaatatt
    25981 ggcttcagta tgatgccggc taccaaattt agctggaatt tgaaatgcgc caactacgat
    26041 ttcaagatgc ctctttggct tacaggagga attgaatttg tgaaaccgga atttaccctt
    26101 gccacagaag ccgaatggga acaaacaagc gatatttgtg atagttttaa tgatcgtttt
    26161 actttcaaag ccggtttgga aaaaatgagc gataaattag tttaccgtct tggctatcat
    26221 tttacttcca atgtttacag cggtatgatt cgtttggctc aaaatgttgt taatcccgat
    26281 accagtaatg cctggaatac agttccgcag gcagttttca ttgaagataa tgcacagcat
    26341 tctttaacgg cgggcttaag ttatcgtttt aaaaatggta tatttaatct ctctgcgatg
    26401 caagttattt tgggagatgt gaataaaacc cagataaatg tctccctttg tttctattta
    26461 aattatatat tccggaagag ggaaattcat ctaaatgaat aaaccagcag tagaaattcg
    26521 cagaattgac tcctacgatt tacctgttat agaagaggca gtagccgatt tcttcacgga
    26581 aataaaaagc cctaaaataa atcatagtaa aagggtatta attaaaccca atgccttggg
    26641 agcttatcct cccgaaagag cagtaactac acatcctata gtgctggaag caattattcg
    26701 ttattttctg gacaggaaaa aagagatttg gtttggtgac agtccaggta attctgttgc
    26761 ttttgaaact gtctggcaaa cctgcggatt ttcttctctg gcagaaaaat atcatcttaa
    26821 agtagtcaat ttttctactt tgggctttag agaattaagc tataaggata ttagcataaa
    26881 ggttagcgaa gctctgttcc aatgtggaat cattatcaat gtaggcaaat ataagaccca
    26941 tcaattaacc gcattcacag gagctctgaa aaatcttttc ggctttgttc caggattggc
    27001 gaaaagtgaa tatcatcgtt tatatcccga tactaatagt tttgctgcta tgcttaccgc
    27061 tttgtatgct gtaattggca accggataac ctatagcata attgacggta ttacaggtat
    27121 ggatggcaac ggaccttctg cagggaagcc acatcatttt ggacttcttt tcggttcaac
    27181 ttccattcct gcattggatt atgtagcatc acgcataatg ggttttcagc taaaagatgt
    27241 tccctattta agttccgctt tgattatgga tggcatttta ccttcacaga ttaaaattcc
    27301 tgtttctttc cgcaactaca gcatacctga tgcggatata aatagcgtaa aagtaatcca
    27361 gaaaacgctg aaatatgttc ccggtattgc tcgtaaagca tttcggaaag tgtattactt
    27421 ttatcctgcg gtaagtgaac gctgtaaaca atgtggaata tgcgtaaaga gctgtccggt
    27481 aaaagctatt tcctggcaaa ataataccaa gccctatatt cataaagagc agtgtattaa
    27541 atgtctctgt tgtcatgaac tctgtcccta tcaggcaata gacattaaaa aatcattact
    27601 ggcaaggtta ggtgtataat gaaatccaaa acgaaaaagc ttagtcctac tttgcgtttt
    27661 gccttttggg caataatagt cctgttgata atctggattc tatttttagc taataacagc
    27721 tttctgaata cctggaaatt gaaacgccgg gtagaacagc tggaaaaaga aacctccatc
    27781 ctcaaagctc aaaatgatag tttggcacaa gaaaatgaac gcttaaagac cgaccccgaa
    27841 gccgctgaaa aagctgcacg ggagaatttt gggctgacta aacctgatga aaccgttttt
    27901 cgtttcgtcc cggcaaaaga agatgaaccc ccaaaaaaat agccaacctc atatttacaa
    27961 tatttccctg ttcacaaatg tggaacagga tactcgtaat aagctgaaga aattagttca
    28021 cgatttgatt ttggcaaatg aggacagccg tctgctaatt atagaaaaga ttgcggatat
    28081 agtgattcct gcctgttcag ggaacctgga taatcagctt ttcagtgaat tttccggttt
    28141 gcttattgaa tatctgctgg attatttcct ggagctgaaa gcaacacagg aaagcgagtt
    28201 ttatcatttt ctcagttttc ttagtcattt ccccgtaaca gaggattcgc cttattatcc
    28261 ttatgtgcaa aagattatga atgaccataa ggaaagcgaa ccggaattaa tgaatatctt
    28321 taatcgcctt cagttgcttt atcttttaac ggaaaacggg gattttggga atgcggaaga
    28381 attgctggaa gaactggaac ctgttgaaaa aaataaacat cttgagcttt gggctttatt
    28441 ccagctttcc caaacccgaa tctatcgtca tcaaaataaa cctgccgagc ttttggagac
    28501 gcagttaaac ctgattttag aagcatatat aagggatagc agtgatagcg ccatcaattt
    28561 tattatccgt tggcttattt ctattaactg gtttaagcag aatatcatca aaaaaacctt
    28621 attgctgaga attaaggatt accttttaga acagaaatcc ttgaacacag caatggtatt
    28681 gtatgaattg ttttcaatgg aagaccgtct tgtgccttcc gtggaaaaga tagaatatca
    28741 acggatactg attaaatatc ctgtttcaat gctgaatgtt caacaacttc atacgcttta
    28801 tttttttgcc ggatactata actgtggtgt gctttccaat ttcaaggatt ccattcagaa
    28861 ttatcaatat tccaattact ttttgcataa aagctgggat tcttttatcc atctttcccg
    28921 ctttatgcga gaacaccttg atccttcgcg tttttataat gctgttccct atctggaaag
    28981 tcgtatcaag gaactcagca atcaggcatc tttacagaat aatgcttatg tagagagctt
    29041 acaagcaaac ttctataaaa ttgaagaact ctatgagaaa gtgggtgaac tttctttaac
    29101 cgatagttta accggtttaa gaaatagacg atacttggaa ggaaacctct ttcaaatggt
    29161 aattttagcg gctcggcata atgttcctgt ttgtttcagt atgatagata ttgatttctt
    29221 caagctggta aatgataatt acggtcactt ggcaggggat tttgtgttga aagaactggc
    29281 tcgtattctc aattctgagt tccgtaaaag tgatgttgtt atccgttatg gaggcgatga
    29341 gtttttagtt attctttttg attccgacca gaaattaagc tattccatta tggaagacct
    29401 ccgccaaaaa attgaggcat attcctttga ttacaacaat cagattatta ctttcacaat
    29461 tagcatcgga attgcctgtg attttcatat ttcctctcag cccaaaaacc tcgccaaatg
    29521 cattgcttgt gctgatcaag catgttatgt agcaaaaact aatggacgca accgggttga
    29581 actctatcaa tcaccaactg gagctaccga taataactgt tagaataata tcaataattt
    29641 ctctcttgac atatttccct attacctgct ttgagtaaac acaaaaggag taataatggc
    29701 taaggttacc gtaaaaaacc tgaataaata ttttgataat ggtgttcatg ctgttaaaga
    29761 tgtaactttt actgcggaag ataaggaatt tgtagttctt gtaggtcctt ccggttgtgg
    29821 aaagaccaca attttaagaa tgatagctgg tttggaagag gtctcttctg gggagattta
    29881 tattggcgat gttttggtta ataatgtgcc tcccaaagaa cgagatattg ctatggtttt
    29941 tcagaattat gctctctatc cgcatatgac ggtgtttgat aatatggctt ttgctcttaa
    30001 aatgaggcat attcccaaag aagatataaa gagaattgtt tatgacgcgg caaaattatt
    30061 gggtatagaa tcactcctga aaagaaagcc aggtcagctt tccgggggtc aaagacagcg
    30121 tgttgccgtt ggaagagcca tagttcgcaa tccgaaggtc tttttatttg atgaacccct
    30181 ttccaatctg gatgccaaat tgcgtgtggc aatgcgggca gagattgtta aattgcatca
    30241 tactattggc aatactatga tttatgttac gcacgatcaa gtggaagcaa tgactatggc
    30301 agaccgcatt gttgttatga aagatggtgt tattcagcaa atagataccc ccttaaatat
    30361 ctataataat cctgcaaata tgtttgttgc cggctttatt ggcagtcctg ccatcaatat
    30421 gacgaaaggc aaattgatta gaaaagaaag tgatttgtat tttgatagtg aagattatca
    30481 gctgaaactt actcctcgcc aggcagaagt tctccagaat tatacaaata aagatatcat
    30541 tatgggcatc agacctgaag atatctacga ttcacgtttt gacgctatgg ctgaatcacc
    30601 ccagaaattt attactaaag tggattttgt agaacccttg ggaaatgaat atcatgttat
    30661 tttaactaca gcaaataacg aatatacagc gcgttttgat cctaaggact tacctaaaat
    30721 gggacaagac ctcggcgtta cttttgatat ggctaaagca catttctttg atccggagag
    30781 cgaagtaaac ttattttgag cccataacag cttatcagct aaagtgttaa tgctatgagg
    30841 tgcaaaatgg ggaaacaata tattcacatc ctgagcgtaa cttttatcct gttttgttcc
    30901 aatcctttcc tgttttctgt tacaacggaa aattccgctt ctgctaatgc cttatcagga
    30961 atcactatgt tatcggcttc acctgcggat tatactcttt ctcctgtaat tggggaaagc
    31021 ggaatttgtt ttagttggca tcaaccattt ggcataagcg aaatcaatgt ttatggattg
    31081 catagtgcct ttcccgtaaa accgttgata gtagctacgg gaattgacta tctggcgcat
    31141 acggattatc gctggcaaaa tgaatatttg gctttaagcg caagtttttc cttttttcgc
    31201 ataggtgcaa ctcagcattt aatctacgaa aaaatagcgg agcaaagctg gtatacctgg
    31261 gataacgatt ttgctctttc ttttcagaat aaaaggttcg gaagcgaaat acgctgtaat
    31321 aatatccgca cggaagaaag gacatttacc ctttctgcca attttaacgc caatgaaaat
    31381 tcctcctttg ctacatcata ttccttcaga cccaaggaaa aggatagtta ttctctggca
    31441 acttctcttt ccatagcaaa acccttaata attcaaagtt cctggcaaag tgaacccgcg
    31501 cgttttggac tgggactgaa agtgctaata ggtaaagtga atttgatgta taccgtgcgt
    31561 actcatacgg aacttgattt aactcatatt gtggatttgg gctctgcctg gtgaaatttt
    31621 ttggctggtt tcttgtcctc tttacttttt gctgtgttcc tttatttagc caaatagaag
    31681 aaataccgga gctggaatcc gaaaccttgg aagataactg gctgaatcaa ctttacacgg
    31741 aactggaaac aggaaagcat aaggtagacc ttcagagccg gacaaaatat caggagacct
    31801 ttgctaatgg actggcaaat cttcaattcc gccagaaaga tatgcgcttt aaccttaatc
    31861 tttataaaaa ggaaaattca gacctttatg ccaatttcca gctatcggct atgccttcta
    31921 aaaacaaacc ctcggaaatt cattttggtt cctttcgtcc tgcttttggt atgggaacta
    31981 ttctgaaaaa aagcggagga aattcccttt ttgccataaa acgagcaggg catcctgctt
    32041 ccttttctcc ttttggaaca ggagctattt tgcgggtaaa caatttttcc gctttcttta
    32101 tggcttccgg tcaaaagcgc aatgcttctc tgaaagaagg caaaatcggg tcactttata
    32161 aatcggttca gtccgagaaa acttccgttc aggaagaaat tatcgccgga ggactggagt
    32221 atcactataa aaacaataat tttgccttgt tggcttataa ccagaattac gatcgggact
    32281 ttgccgatac gctttgggta aaaaaactga atgcctactc aatttcggct aaaacaaccg
    32341 gggaaaacta tgttttatct gcagaaatag ctcgtctgga agaaaacatt gcttttcaat
    32401 cctccgttaa attcatttat aattatattt ctcaggaact cagttattct tatagacagg
    32461 gaaaacaatt gcctgcttat gctgccaagc cctgtctatt aagcaatcaa ggagaaaatc
    32521 gggaaattga ctggaatttg gattatcagt taccgaaaaa tatgtccctc ggtttacgct
    32581 atgctctaat gcgtaaaaat aacgccctta aaagtccgaa ctggaattca cgaggaatat
    32641 tcaatcttac ttttaatccc tcttatacca ttataaattt acagttaacc ctgctggata
    32701 aggaaatcgt aactgaaacg gattcaagct atatagctac tcttcctgtc cattatcgtt
    32761 tgcggtttaa gatagaccac gaattaaatc cgaaactcac cttatctctt ttgctccgtt
    32821 atcattatga agataaaatt gaacgccgaa acaacagctt ttattgggaa aacgcttttc
    32881 actatcacag gacaaaatgg caaatttctg ccggaattaa aacctggcaa acccttactt
    32941 ctcttattct gcccgaaaca gacacggaaa atccggaggg atgcatttgt gccaccagtg
    33001 aggatgatag ggtctttgcc aaagtaacct ataaaggaaa agtaattaac ctgaaaacgg
    33061 aatggcaaca atcctggcta aacggaaatc gcactctcta cctttcagta ggattgtagc
    33121 attatcggca ataaggattt tatagatgat actgatttat acggattaaa tttccgctga
    33181 ataaaataga gataaaagat tgtgagagtt gaaagcaaag agatcagagc agaaaatgaa
    33241 ggggtttcaa aaacagaaag caaatagcta aaagccaaga gatgaagata ccggaaggta
    33301 aatttcctcg taaatcacta ttagcgcaaa gtttagaggg ttgaaagggt ttagaaggtt
    33361 gagcaagaat acggctctta tctattcaat tatatatttc ccttctacat ttgtaactac
    33421 ttctaaatat agacagttcc attttcttcc ttaatagcat tgcttaccca ttgcttaccc
    33481 attactaatg ccatccgtaa tgggtaagca atgggtaggc aatgccatct gcctaaaggt
    33541 tttccccttt ttccaaggtc acaaagaagt ccctgcctat attttcaagg gtataatgcc
    33601 agggtgaaat atccgaaaac aggttttcca gttgtttttc gttataaagg taaagtggac
    33661 acttaaattt atagcgtatt ttccgttgcc aagccaaaaa acctttatcg gaaggaaaac
    33721 tgaaaagaac tttatcactt gccaaacgca ttgttttttc aataacctgt ttgggatttt
    33781 tcatataatc cataaaaccc attaaaataa ggtaattata ctgcttatcc tccggcaggg
    33841 tattaaaatc ggcaatttca aaccggcaga tattttccac ccctgctttc tttgcctctt
    33901 gtctggctat ttctaccatt gaaggtgcaa aatcaatacc gtaaacaaaa ttcggagaac
    33961 cttttgctaa agttatagaa taaattccag gaccacaacc aacatccaga attgtctttc
    34021 cttccaaggg gttacaagca acaattgtct tttgaaaacg gattaacatt gtctgccgaa
    34081 accatttatt gattaagcgt tgaaaaggtc ctgtgccgct accgtaaata gcattaaaat
    34141 caattgaata gcgatcaaag aaagaggcgg ttttattttc ctgcatttaa ttatccttca
    34201 tagttttaat atagttcctg ctgactacaa atcttcctta ttaagtggag gatgaggaaa
    34261 tcctttttcc cggaaagcag gtttctatat actgccggca ggttataaac ttactgctgg
    34321 aaagtatagc ttccagctta ggttgcaccg tattcaggtt tacataactc ttgaaatatg
    34381 ccattttgtt cattttcaaa cgcggatgtt gcggttccat ttctctggga tgaatgtaaa
    34441 aaagagtagg aattccttgt tttgccagct ttttgtgcat atacaaaata acccgcttag
    34501 ggaaaagacg caaatagcca cccccgaaaa agcaaatatc tttaccgcaa aagggcgcta
    34561 cagaaatcgg aaattcacat atatcacctt tttctgtcgt tatccaatag ggctttttct
    34621 gcgttgtttt gtaaccgcca tagtcccttt ttacgggaaa aacagaagaa tcggcaataa
    34681 aacctgtctc tgccaaggtc tcaaaaaacc agggacttgc ttccgttaaa gaaaaactgg
    34741 gactcctgaa agctgtaacc tgctcggaac aaatatcttc cagaatgttt ttggattgta
    34801 gcaaatcggt ggtaaattca tccttgctca ttttataaac aatccgatga aacattccat
    34861 gcgaggcaat ttcgtgtccc cttttctgtg cttctttcac taaatgcgga tattttttgg
    34921 ctacataacc caaaaagaaa caggttgcct tcacctgata ggtatccagc aagtccagca
    34981 acttcaaaaa accgctttct acaatactgg gtaaagaatc ccattcggaa acaggtggtt
    35041 cacctctttc ttcggttaca ttaaaataat cctccacatc aatagtgaag atggactttg
    35101 gatattctct cataaatttt tagctaaatt cctatataga tataatatca tttctggaat
    35161 gaaaagtcct ttgttattcc tgctgaaaca agaattcaca gaaggtaaaa aataattatg
    35221 cattttcaaa cagaaatttt aataaattcc ctaattgaag aagagtgaaa aatgtcaact
    35281 gctttcttgg ggaatattat atacaccgaa aacactgaaa atattgttta tttacactat
    35341 tagaaaccga ggaaagaatg aattatcctt tttttctcgg tgttctcagt gtcttcagtg
    35401 ttctcggtgt aaaaatctct acaatttaca gcttacgcat atattccagc aagtccacta
    35461 ctctgttaga atatccatat tcgttatcat accagctgaa gaccttcacc attttacctt
    35521 ttgtataagt tatcaaggca tcaaaaatgg aggaatagct attaccaacg atgtctatag
    35581 acacaattgg ctcttccgta tacattaaat agccctttag atatgtttcc gccgcttctt
    35641 tcatagccat atttacttct tctttggtaa cttccacttc cagattggca caaagatcaa
    35701 ctaaagaacc atccggggta ggaactctaa ttgctactcc atccagtttg ccttttaatt
    35761 caggaataac caaaccgata gctttggcag ctcctgtgga agttggaatc atactcattg
    35821 cggcagctct ggctctacgc aaatcattat ggggcaggtc taaaattctt tggtcgttag
    35881 tatagctgtg aatagtagtc attaagccat taaggatacc aaatttgtcc tgcagaactt
    35941 ttgctactgg agccagacaa ttggtagtgc aggaagcatt ggaaacaatt gtatgttcag
    36001 cttttaaaga ggtatggttt actcccatca cgattgttgc atccacttca tcttttgccg
    36061 gagcagttaa aataaccttt ttagcacccg ctttcaagtg tttggatgct ttttctttag
    36121 aggtaaagat accggtggat tcaatcacga attccactcc caaatccttc cagggaagaa
    36181 cttcagggtc tttttccgca aaaatcctga acttttttcc attcacaacc aaataattct
    36241 cttcgctgga aacattgcca ggatatatct tatgcacact gtcatattta aatagatgtg
    36301 ccagcgtttt agcatccgta agatcattta tagccacaac attcagctca ggatggctat
    36361 ccaaaattac ccgtaaaacc agacgtccaa tgcgtccgaa accgttgatt gctacatttg
    36421 tcatcatatt tctccttata agtatttata tatctattag atagattgct accggaactg
    36481 ccggaaaatg cctttttaac ctttcaatat cgttaatcag ttgttctcca taagaaacac
    36541 ctgtggacat tgacttgtgt aattggtaac agggaactat ttctattccc acatctgctt
    36601 tgttttcatt gaaaaagtat tgaaatttgg caaggtcgta aaacataaaa gggtattttc
    36661 ccaattgcac ttccacaaga actttcccta aacagaagtc aatttgttta taacaaccgg
    36721 ttattttcgg ttcgtaattt ggtaattcta tactaaaagt atctctcagt tctttataac
    36781 ctaataattg aaatttgtct ttgaattgtt tatttatatc tttaggagaa aaaagcgatt
    36841 tgccactcat tgttttttct ttactaatct tggttttgtg aggtttaata tcctttataa
    36901 tgtttaatac ttcttccata attttaggat atcttactaa taatatttcc aaaccgccaa
    36961 gatgtgagta ttcatataca atattcactt ttcattactc caaaccagat caagttctga
    37021 ctgttcttct tttattttgg ttaaatttac cgtaataggt ttgaatgaaa taggatgttc
    37081 gggatcaaag atcggcctat tcatagggcg tattttcaat tcaccggaaa tggcactatt
    37141 aattctttgc agggcaattt cataatattc atcaagaagt tccgctccac agcttttccg
    37201 gttatgaata agcgaagcaa tttgcgttgt tcctgttccc ataaaagggt ctaaagtcca
    37261 gtcattttca ttggtcatag ataaaattag gcgttctacc aattctacag gaaattgggc
    37321 aggatgaatt gttttttcaa tatgattagc tttcacattg ggaatattcc atacatcgga
    37381 aggatttttg cctaaaggat tgcaagataa ttctcccttt ttgtctccct taaaatattt
    37441 cttattagga tacttctggg gaattcggac tgggtctaaa ttgaagatat attcatcact
    37501 tttggtaaac cagagaatta cttcatagcg tcctgaaaac cttttggaag aatgcaaacc
    37561 atgcccgaaa tgccagatta ttctatttct tagttttaag cccgattcag caaaaacagg
    37621 ataaagtaaa atatccagag ggataatttc tccgttttca atataatttc ctacctgcca
    37681 acagatactt ccactttttt ttagaactct acagcattct gtaataacct ttttctgcca
    37741 gtcaatatat tcatctaagg gttggcgttt ttcatatttt ttgcctatat tatagggagg
    37801 tgaagtaact actaactgaa tagcggcatc cggtatggaa tcaagtagtt ccaaacaatc
    37861 accctgaaat agacagagaa tggaatcaga gttccattta ttgcttattt taaccatatc
    37921 agctataaca aaatactgtt attagcgcta ccggacattt tgcagaattc cataaccgta
    37981 ttctgcattt tctcttcccc tttgattaac catttacgag ggtcaaactt acttttatta
    38041 ggtatatagg cagcagggtc aacatcagga gggcaaacaa tggcatattt ttccttttcc
    38101 caatattctt gaatagcaga agccatatcc atctggtaat gagtatcttt attgattttc
    38161 accacaccat tggcaatagc tgtttgttgt tgccaatcag ttaatccgga agctccatgt
    38221 aaaaccagtc ctctttccgc tccattttta atcagcaggt catcaatttc cttaattaaa
    38281 tctaaatgca gaactacatt ttcaccttta gtaacgccat gatttgtgcc tacggaagca
    38341 gcaaaaaggt ctacattggt ctttattaca aattccaatg cctcttcggg tttggtatag
    38401 agctcatctt cggaaacaat ttcatcttca gtacccttga tatgaccgat ttcaccttct
    38461 actaaaatgt tatatttatg tgccaaatcc acaacctctt tagtaatccg aatattatcc
    38521 tccagttttt ctgccgaggc atcaatcatt acagaggaaa cgaggtcgtt ttcaatacag
    38581 aacttaataa aatcaaaata gttaggtgtg gcatggtcaa tatgcaatcc aattccacat
    38641 ttgggaaact gagcagaaaa gatttttacc attgcggaaa tcatttttgc ccctgtatgc
    38701 aaatcctgag aagtgcctgc tgcatattta caggcaccag tactcatttg cattaagcca
    38761 tcagaattga cagccgcgta accttgaatg agagctctta cctgtgtgtc aaaaacaaca
    38821 ttggatgcga tgataccaaa acgctgtttt ttagctttga ggaagacctc tttaagttgt
    38881 gttccggtta aaaacatttt atacctccac ttgtgtttag ttcttataag ataaactatc
    38941 caaatgttga tataaagtca agggaaatct gcagaagaaa gaaggtggag aaaaaatctc
    39001 cacctcttta ttttattaag aatagggttt cttgtttagt tagttgcaat cacccgatag
    39061 aatttcttgc taacagcggt tccgctatag aataaattgg gagttgtggt tacggtagac
    39121 caggtgccat aaggatcatc tgcggattca attctgtagt tagtagctcc aggaacctga
    39181 ttccagctaa ggtttatcat tccagctact tgagaaatat taacaatcgg ggcttccaag
    39241 attccagcag cgggagttat atctgccaga aaacgcggac tgaattgcat cgtgtcatta
    39301 tattgacctc ccagacagac gatatctaca gggtctgttg gaatagcggt tccggcataa
    39361 tcggcattgg ggaaagttct cataacagcc gttccggaag catcggtagc agtaatattt
    39421 tctgctgtag cagcaaaaac gccagtagca ttgggtgtta aagttacact atatatctta
    39481 agcagttttg cctgatcggc agatgtgata gttgctaaag ttctcgttac aggaacaatt
    39541 gtattattat gagaggtggc agctcctgga tcggcaatag gaacaaattg tagtaatcca
    39601 gaataagaag ataaagttcc tgttattccg gttatgccat cgtagaggtt ataagtagtg
    39661 gttattttgc cgttaggatc atcaataact atagcagctg ttgcatcctg aacatatttt
    39721 tgattacgat ttgcctgctg gaaagtaaga actgcttcac ccgtaaggcg ataggcacta
    39781 cctaaagttc cttctctcag ttgtgctata gtagaaacat tttccggaaa atcataaaaa
    39841 gcagtagcaa ttgcagaggg cgaataaccg gtagcataac ctttagcttt gatagtggta
    39901 tcagaactga caggtattgg agtgctgtaa acaggagagg cctctgtggg ctctgaacca
    39961 tctgttgtat agtgaattgt tgctcctggt gtaattgtgc tgatagttac attgatggga
    40021 gtagcataag ccccagcagg aggattgaaa acaggcataa ctaccatatt gccaaaggta
    40081 tgcattccca gattatcagt ataatcctgg gcataaacaa tccattcact atcatcggca
    40141 ttagttccag cactgatagc ccaatccaaa ttgccctgag taattgtggg tttacggatt
    40201 aaggtatgtt cagcagtagc atttgtaact cctgccacat tccatgccgt tcctggatct
    40261 gtgccaatgg taccaataac atctatcaag gtttcggttg tacctaattt atataaacca
    40321 agagcatcgt ctccattata aaaagtaacg gaagatgttg tatctgctaa tcccaaaata
    40381 gtggcgtttg cagaagtatt ggcaattaca taaacatcaa ggtgagcaag agttcctgtt
    40441 aaatttaatg tatttgaagg tgtggcagcg ccattggcat aaagacaaac cttgtaattg
    40501 ctcaagtcca ccggtccacc tgttccgttg aaaatttcaa tagctttatt actactgctg
    40561 ccttctatat attcagagaa gaacaaatca cttgccattc cgggttgaga tgtggcagtt
    40621 ccgctaacgg gaagttgata ttcattagca taagtagtat tatggtaaat tattccgttg
    40681 aatattccag aaccagcggc attcatccgt acataaacat ttccgctaaa attattgctc
    40741 agcgttaaac ttattcccca gttacctgtt cccgtagaac ttaattcaaa aggataggaa
    40801 gtagttattt gaagagaatc aattgctccc ggagcactta aagtgtatga ttgaactgca
    40861 gaaggagttc ctgcttcaga agaaaaagga gttaaattag cagtaatatt ccaggttaca
    40921 ggcgtgggaa gagctgctcc tgttactcta acatttacag gttcggcatc tccactggta
    40981 tgaacaatat agccctcata ttcaccgatg gcgtttgcca gcatccgcac ataaatttgc
    41041 gagtgaaaat cggcgggaac agttatactg gaagcccatc cttctatttg agaagtagaa
    41101 acctgaaagc cgttgggggc tgtaacagtt attccaccta cgatattagt tccccataaa
    41161 gtataatctc tggtttcgtc tgagggaaga tttacaatgc ttaccaaagg atcaagctcg
    41221 gttgtgctgg tatgaataac tggagtttcc ataggagacc cttcccaaag tttagcccag
    41281 ttattggtta ctcggatgcc atcaattctg gctataggac cattagtacc ttgacgaata
    41341 gccactgctc caattgctcc tatatctgtt cctgttaaat cagcagccgt aagttgaggt
    41401 gcaggttcag tttctccgat aactggatta acccacatat agacctcatc attagtagca
    41461 cctgaaacaa ttacatattt
//
LOCUS       DAOOZV010000011        39143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1178_length_39143_cov_54.662480, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000011 DAOOZV010000000
VERSION     DAOOZV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 39143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39143
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_39143_cov_54.662480"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1294)
                     /locus_tag="PLW08_02265"
     CDS             complement(2..1294)
                     /locus_tag="PLW08_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPL51458.1"
                     /translation="MDEKFLYHIWDEGHLAPNLKTVSGKDLKVNYQGQFNTFRGPDFI
                     NAIISIDGEDLQGAVEIHKNTQDWIKHSHQEDVFYNQVILHIVLHNDSPLPFTMKENG
                     ELVEILELKNQLSDEIQKLLADIGDKNLASGSDYCDLLSAIDNDRLLSILTISGKQRF
                     MSKVRRFNASLALSDFDQILYEGMMEAAGYDKNKFNLFQLAQSIPFAKLKEWHKEGLK
                     SDEMISIFLGSSGLLLKSRNRLNGELYATLSRSFESQRFYARKINVDWQLFRIRPGNH
                     PVFRLILLSEFLYNCLQEGVLHFFLKTVELAKPNPQKRYQTFCKQFKPGQEGLLKNNT
                     GLGITVINNIYINIYLPILYLYYQKLANTEMTESVLESYLSFKALPENYITRFMCNHI
                     NSSQVSIVNKKTLYQQGLIDIYYRFCRYHLCEECVKQT"
     gene            complement(1294..1965)
                     /gene="mtgA"
                     /locus_tag="PLW08_02270"
     CDS             complement(1294..1965)
                     /gene="mtgA"
                     /locus_tag="PLW08_02270"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPL51459.1"
                     /translation="MKRRRSIWLRIIRFIVIIHLCYWVIIAFFSLLYNFVNPPVTPLM
                     LQRYLVRGYPVHKRYYVPLEKIPERTQSMLIAIEDANFYKHFGFEWKMVKEAYQRNKK
                     AGKIRFGASTISNQLARTIFLTTNRSYLRKYLEIQATVIMEICMSKKRMLELYFNYVE
                     WGKGIYGIETASRYYYGKSCRNLTRTQTMKLISILTNPLRYTPETYYKSASARQRYNL
                     LQRYF"
     gene            complement(2022..2291)
                     /gene="rpsT"
                     /locus_tag="PLW08_02275"
     CDS             complement(2022..2291)
                     /gene="rpsT"
                     /locus_tag="PLW08_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPL51460.1"
                     /translation="MPQHKSPLKRMKTDKKRAARNNYVRNTIRTLSKKIHSDTTPEEK
                     EKLLSELYSKLDKAVKKGVIHKRTASRRKSRLADYVNKAKVEKTS"
     gene            2585..4642
                     /gene="fusA"
                     /locus_tag="PLW08_02280"
     CDS             2585..4642
                     /gene="fusA"
                     /locus_tag="PLW08_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL51461.1"
                     /translation="MKDNEMKKIRNLLFIGASGAGKTTLAEQIFYLTKTTNRLGRIEE
                     GNTIMDFDPEEIAKNMSLGLSIGYVKYLDHKINILDTPGCPDFVGDAIVAIPAVENAI
                     IVANAAGGYEVGLELALEQIEERKMGKVFVVNRMDNDNADYDKTLEAIAENTDIKPVK
                     IYLPIGKEGAFEGVVDIIKRKAIKAGKESEVPTNLAEAVEEARIHLMEAVAETDEDLL
                     NEYLENMELPEDKLIEGLKKGILSGEISPAFVCSAGTGEGVLALLNGIINYLPSPAEA
                     NEIEVLKENEKTAFICQPDGELLGYIFKLYADPGMGDFVYFRLFSGTLKTGMEFYIPE
                     KDAKDKAGTMYFMLGKNRNDCNEIKAGDIGALLKLKNAKVMNSIVSLGSKISVIPVEL
                     PTPTTWQTIKAVNQADEDKIGTVLQRVIAEDPTIRYELNVETHENVLSGMGEQQLQLV
                     LKKLKNRYKVDAILKEPRIPYKETIMTSAESNYKHKKQSGGRGQYAEVYFRIKPTERG
                     EGFKFINSIVGGVIPSNYIPAIEKGLWETMEKGIIAGYPVVDISVEVYYGSYHEVDSS
                     EIAFKIASSMALKDAFKKAKPILLEPIHELIIIVPSEYMGDVMGDISTRRGKILGMEQ
                     HSKKQYLTAQMPLAEMYSYYPALKSFTQGRGRFIQKFSHYEKVPDDIAQKVISAWQEQ
                     TNE"
     gene            4955..5707
                     /locus_tag="PLW08_02285"
     CDS             4955..5707
                     /locus_tag="PLW08_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPL51462.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQVFTRLVKEIILAAKTGGGDPD
                     MNPRLRTAINTAKENNMPKENIERAIKRGTGEIEGAAYEEVIYEGYGHNGVGIVVEVM
                     TDNKKRSVSELRHTFSKYGGNLAETGSVAWNFEQKGFFNVPSAGLDEDEFMLKALEAG
                     ADDVELNDDNFDVYTSPADFHIVIGNLEKEGYPIHNAELTRVPKNTIAVDDDTAEKLM
                     RLIESLEELDDVQKVYANFEVSDEVMEKLSQA"
     gene            5716..6195
                     /gene="ruvC"
                     /locus_tag="PLW08_02290"
     CDS             5716..6195
                     /gene="ruvC"
                     /locus_tag="PLW08_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPL51463.1"
                     /translation="MIIIGFDPGSRYCGYGLLQIESRKVLAAGCDVIDVTRGKTLALR
                     LKSLYENIMQILEEYHPEIAAVESIFFHRQVRSVFTLGHSRGVILLALAMTDIPVVEY
                     SPREIKKSVCGNGNASKQQVRYMINQLYPLKESPKRDDAFDALAVATCHYNRMKWLQ"
     gene            6192..6791
                     /gene="ruvA"
                     /locus_tag="PLW08_02295"
     CDS             6192..6791
                     /gene="ruvA"
                     /locus_tag="PLW08_02295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPL51464.1"
                     /translation="MIQYISGTLTFKTPVLAIIETMGIGWELKIPISTYEKLPTAGKP
                     CKLLTYLHISQDDIRIFGFATEAERELFVMLNKIGGIGPKIALSILSTLSIPTFIKAV
                     NSGEEGLLTKVPGIGKKSAQRLIVELKDDVHKLLNHIDQKELQIGDVSSEVESALLSL
                     GFNLHQIRKELALMDEPMQKLNTEELIKEIIKRIYQRNK"
     gene            6788..8104
                     /gene="rsmB"
                     /locus_tag="PLW08_02300"
     CDS             6788..8104
                     /gene="rsmB"
                     /locus_tag="PLW08_02300"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPL51465.1"
                     /translation="MNFRSEAYTTILKVLKDKEYSDTLLHQRAKKLKNAGEDPALFYN
                     LVKGVIKLYLKLDYILQQYVDKKKYTDTDIKIKVLLYMGLYQLLYLNSIPDYAAVNET
                     VELAKTLFNPQVADFVNAVLRNYLRNPEVSYPEEPALRIAYEHSFPPELIKQWLDLWG
                     EENTEYLAMFFNENPELHIRVNSTATTPEKLKAYFAKRNIVITPSQASPMMFHTAQTA
                     EVLEDVAFSEGYFSVQDTSAALIVELLDPQKEESVLDLFAAPGGKCSYIAERMDNTGE
                     VIAVDKIPSKMKLLKQAANRLQLTNIKMVVTDAFKYGPVAPAFHRVLVDVPCSGWGVF
                     GRKSDLRWQAHQNIPELIKLQEKALDYAANFVAPGGFLVYSTCTMNPKENEDQISAFL
                     KKNRNFELIPADAFIPERYTYAGYLKTIPFKHNMDGAFAAKLQRRK"
     gene            8067..8699
                     /locus_tag="PLW08_02305"
     CDS             8067..8699
                     /locus_tag="PLW08_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPL51466.1"
                     /translation="MVLLQPNYNAGNKNIIARSKSPTGTKKAGYIIGIGIGIIIVTAF
                     VFSQILFPVILGRTPKVEVPSVVGMTLVQAKRVLQEEKLHVIVKDSFFSDVAAAEVVL
                     EQNPVAGEKIRQDGTVYLVISKGSSTVTLPSLIGKPFQEAFIILRNLDLYSSVVDSTY
                     SDIYPVNTVIRSIPAAGEKALKKSTVKLILSRGPEPVADTLNYEIPIYYY"
     gene            8723..9178
                     /locus_tag="PLW08_02310"
     CDS             8723..9178
                     /locus_tag="PLW08_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51467.1"
                     /translation="MKETLKEAKIRSRMMPGALTITGFLGTDQRPLAEIIAADEQELK
                     KLNRSVEEIAERMKYFSNATFDAYNNTVVIDDTYEVHTESTKGTMTCPYGHGGLYRKY
                     VTTLTNRKNNLTVRWNAIDIHLIEAHHFFEGKGSPFRLEPEILVKALFS"
     gene            complement(9320..10528)
                     /locus_tag="PLW08_02315"
     CDS             complement(9320..10528)
                     /locus_tag="PLW08_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPL51468.1"
                     /translation="MKILLITYYFPPCGGASVQRWLRFIRALTKKGVDIYVVTTLNGD
                     YPYLDESLLAKIPSSVKVYRSKPLRFGKLWNILGQKELPYGSLQNKASDSSLKKILYW
                     LRLNFIVPDMRIGWNPSAYKNALQVLRQDKIDYVITTGPPHSTHLVGMKLKKHFGIEW
                     RTDFRDPMSEIYYLKINPPCRITLALHKYLERKIIRTADLNYIFSKVIADALPDGKKK
                     VLYNGFDPDDFKGLHYSRTDKFRIKYVGQLTTGRDASPLMKALSSLQLPLLEFSLIGT
                     RDFPETDFPVIKIPFLPHSKALEELVNAELLILIIDTCKSKEGLLPSKLFEYMASRTP
                     ILCISEPAGEVYTLICKTESGFVSKDSEEIANYILGLYNKFLEGDNLRTKGDISFVDV
                     NNQVNIFLES"
     gene            complement(10525..11610)
                     /locus_tag="PLW08_02320"
     CDS             complement(10525..11610)
                     /locus_tag="PLW08_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL51469.1"
                     /translation="MKIAILGPAPPFRGGISRFALSLARAFKDTGHNVQFFNFREQYP
                     KLLFPGKGQYDSELPANEFVNHQILTPWLPKTWKATIHELKIYRPEAIIVSWFLPYFA
                     PAYGYILRQITCSRKVILAHNIASHEKWLGEKYFLQYAFKPATKIVTLSKATLDELHR
                     KLPLYISKKGVSGFHPCYDMYNDNISATRKDNTLFFFGLIKPYKGLDILLQAMPKVIE
                     AFPDVKLIIAGEVYGNKNIYIEQIQALGIDNNVECYFRYLGDPEISYFFNQSCLCILP
                     YKSASQSGVIATSYSFGVPVLATKVGGLGEYVIEGKTGYLVPPDNPSALAEAIINHLQ
                     QKPDMSQAINEYTKRYSWKGLAELLIK"
     gene            complement(11607..12584)
                     /locus_tag="PLW08_02325"
     CDS             complement(11607..12584)
                     /locus_tag="PLW08_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPL51470.1"
                     /translation="MKKNILIILTGGTIAMKASDPAGVIPSPEFAEFLRQFPQLNSVA
                     NVEVMDYLNIPSPYMTPEMMFELAELIDLKIIDYDGVVITHGTDTLEETAFLCDLVLT
                     TRKPVIFTAAMRSGDEIGLDGPRNIIGSVRVASHPESIDKGVLVVMNDEIHTARDVVK
                     ADTGKIDAFVSPGYGPIGNVDPDKIVYHRASLYRENVWTEQLDTRVELIKAVAGMDGK
                     FIRCCLEHDIKAIVIEALGRGNLPKTLVPDIEEAIARNVLVIITSRTYTGRVLPEYGY
                     EGGGKHLESIGCILAGDMKGVKARLKLMALFGKYQDADIVKRFFQQSLL"
     gene            complement(12702..13370)
                     /gene="queC"
                     /locus_tag="PLW08_02330"
     CDS             complement(12702..13370)
                     /gene="queC"
                     /locus_tag="PLW08_02330"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPL51471.1"
                     /translation="MTRAIVLLSGGMDSLVTAACAVSECDEVCFLHFNYGQLTEKKEL
                     SSFEALVNYYQPIQAKVINYSWLSEIGGSALTDRNILVQKGILSGSKIPVTYVPFRNA
                     IFLCSAVAWAEVIQATKIYIGAVEEDSSGYPDCREVFFQAFNKVIATGTKGEIPIEIS
                     TPVLHKSKAEIVQLGIELKAPFELSWSCYTENDIACGVCESCLLRKKAFKTAGVEDPL
                     PYRN"
     gene            complement(13472..14881)
                     /gene="hydG"
                     /locus_tag="PLW08_02335"
     CDS             complement(13472..14881)
                     /gene="hydG"
                     /locus_tag="PLW08_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPL51472.1"
                     /translation="MKINTEGLKSFIPTAEIEEHLNKNRFADAIRIRDIVDKSLDKQR
                     LNPDETAALINVTDPELKNLILEGAHTLKERIYGNRIVLFAPLYVGNECINDCVYCGF
                     RISNKECQRATLSKEELIAETEALEDKGHKRLIMVYGEHPKYSPEFIAETVQTVYNVK
                     HNKGEIRRVNINAAPLDIEGFRTVKSVGIGTYQIFQETYYEETYKNLHPRGPKSNFLW
                     RLYGLDRAMQAGIDDLGIGALMGLYNWRFEVMGLLYHTIHLEERFGVGPHTISFPRIE
                     PAIGTDFTKNPPYKVSDEDFKLLIASIRLSVPYTGMILTAREPITVRDEVLRYGISQI
                     DAGSSIGVGDYAHKDEESKRKSQFVLGDTRTLDDVIYELAHSGYIPSFCTSCYRAGRT
                     GEHFMEFAIPGFVKRFCTPNALLTFAEYLYDYASERTKKIGLELIDKEVAKIDDPNMQ
                     SSVKENLAKMKEGIRDLHY"
     gene            complement(14871..15143)
                     /locus_tag="PLW08_02340"
     CDS             complement(14871..15143)
                     /locus_tag="PLW08_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HPL51473.1"
                     /translation="MNAKYHILSIIMEDRESAFHPVNELLHSYAQNILLRVGYPMRDL
                     GVAVIFLIVELSVSEMGAFSGKLGQIKSVKVKVTTLKLEEGEQNEN"
     assembly_gap    15243..15342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15343..15950)
                     /locus_tag="PLW08_02345"
     CDS             complement(<15343..15950)
                     /locus_tag="PLW08_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPL51474.1"
                     /translation="MKFDYHIHTEDSYDSSIKANDLIKRANQLNYDEIAITEHLDLLP
                     QEIKKYGAPSLDRYYKRIKELQNNTSELNVLCGIEVGDYHRVKEYALSLVEDINFDLI
                     LGSVHFLSDATNVAVPLKKPLSEEQVRDYYQQNLLLVSHCNIDVLAHLGVYKRGYNYI
                     PDESAFYPLLKDIFNTMIDRKIALEINYSSLCRGYPSFLPELP"
     gene            complement(15947..16711)
                     /locus_tag="PLW08_02350"
     CDS             complement(15947..16711)
                     /locus_tag="PLW08_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPL51475.1"
                     /translation="MLKIGIDIGSRNTKIVIYDAQTKRIEFSAFQTTEVSVTNGVNNL
                     LKEGYTALGITRKINTIGVTGYGRKLYQEASSILSEISCHTAGCLYYFPHIRTIIDIG
                     GQDSKIITLNKKGKVTDFVMNDKCAAGTGRFLEMTAMRLGCDVSELSLLASKSTQNLT
                     LNSTCVVFAESEIIGMLSSSIPPEDIVRSVHRSIAKRILAQMSVMVWEQPLVFTGGVA
                     LNKDMGKCLSEALNSQLLTPPEPEITAALGAAILAK"
     gene            complement(16714..19377)
                     /locus_tag="PLW08_02355"
     CDS             complement(16714..19377)
                     /locus_tag="PLW08_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPL51476.1"
                     /translation="MNNRINSGLNNFPKPVSRILANGLEVISLQDNSNSVLCLQLYIR
                     TGSVQENKNQRGYSHFIEHLSFKSTKDFPFNGISLFASGLGGMLNAFTDYDCTCYYVN
                     LPAEKLKEGLHILSQLAFQSTFSREDVKTEKEIIVEEIKQYKNDPETDFLEYIQSSYY
                     QKSPLKYPILGSPESIMQADWEALHRFYKNRYIPENAFLIICGDFCQKELDYYLDYYF
                     TPWKSRGKPVKQLTNIEPEINGFRYFFRQKELNENTIAIALPELSEKHPYANALLIAI
                     RYLAIGKSSRLFKRLVEEEKICSSVKVSSLCGILSGASVISITPLSDKYISEVIKLFR
                     TEYSALLNYGIPESEMELIKKDIINSWLFSFEGMENLASLVATEKFVGDLNRLQSYGA
                     EINATTLNDIQQAMYKYWLPEGLAVYYQGAVENVDFAEKAYALKSVSLQQNSNSSPAI
                     LSLKPSLNLEIKPLNQDVKINTEKITPIAEGFYQIILSNGMQVLFKQLKNKSISGFSL
                     STPISQICETTSTIGHNYFCSSLLLYKTQKHSHQELQQFSRENGFNIRLIHHLDTTTF
                     RGKCLSVNLKKALSMLAEIIYLPNFDRNYLSLLTSAALDEIRRDNDIPVSYAYLNWYK
                     MLVGNNSNLFRSSGNPSHIRSLHLKDIQEWYEKWNIGKDFYLGIVGNHKPEEVLELCE
                     QTFGLAKKASQSLYPKPLYSPSTIHFKRKYRKTDQAIIFNGGFACPAVSRDENTAFYV
                     LSQILGGDISSRFYYILREKYGYAYQTGFEFHSLNELGFWSAYAFCDRDDYRNCLTLM
                     QDILYSLTEKEVAEDELENAKQYLIGMNRFEDESVSYTASMMSNLSALGYEPEYYLSR
                     EERIRKVNPEIIQQIARKWLLPENQYIYLLL"
     gene            complement(19382..20155)
                     /gene="folE2"
                     /locus_tag="PLW08_02360"
     CDS             complement(19382..20155)
                     /gene="folE2"
                     /locus_tag="PLW08_02360"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPL51477.1"
                     /translation="MSIPDIQSQADNRKITIDKVGVKGIRYPIVVQDRKKGIQHSVAD
                     LNIYVELPHQRRGTHMSRFLEVLNRYHTEVFIDKLDAFLSELKTTLKADSAYIDIMFP
                     FFIKKQAPVSRVSSLLGYDCSFNASFKDSFELWIGVKVPVTTLCPCSKAISVYGAHNQ
                     RSDIFIKVRYREFVWLEELIQLAEKHSSCEIYPILKRSDEKYVTEKAYNNPKFVEDVV
                     REITLELNKDKRITGFYVEAENFESVHNHNAYALISRNL"
     gene            complement(20152..21156)
                     /locus_tag="PLW08_02365"
     CDS             complement(20152..21156)
                     /locus_tag="PLW08_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPL51478.1"
                     /translation="MNRREIISLIILILVIGFGTYKYLTRSFTELKSQYLMDTIVEIS
                     ATSQSKNVGKQIDAVFNYIRNLEDKLNEFNPQSYLAKINADTLNTVFEMDPDVYNLLT
                     IADSLYKMTEGAFDPTIKPVWDLWGFNAEKPVPPDSLEIKKMLEKVDFSKIKYTPQQL
                     FKPVGMQLTFGAIAKGYILDKAREYMQTLNLDSGFINCRSSMAFMGFQKPQIVYIQHP
                     RRLDDYIASFKVLNQSVSTSGDYQQFYEYQGVRYHHIINPFTGYPVPDVHSVTVLCPS
                     AAWSDGLSTALFLLPPETAISIIKPIPDCEAIIYYTKDDSLVSLKTKGMKKRDLNEKL
                     "
     gene            complement(21170..21967)
                     /locus_tag="PLW08_02370"
     CDS             complement(21170..21967)
                     /locus_tag="PLW08_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51479.1"
                     /translation="MRCKYCNARLAVHDIWCMQCGKQTPVIKNELYSMKSFSETWKAY
                     KSKMSSSLPGAGFAIILGLIPIAVLIWLFSGIIQLECNTATQMLLNLLIKSLAFSLFI
                     PFILIAFNPICKHNDYHLKLSEMFSALRSYPRYFLWTLINALYFCLIYIICFGLPNFA
                     SAPILRLVWLVLVNYWVAIALPSLVLMEEMSYNPWKAIKKSYQHFQDVRWNIYLLALV
                     LAILNALGCLFFWLLLIPEALILPLSLYALRDYTRRLIDFELLNYRL"
     gene            complement(22053..24308)
                     /locus_tag="PLW08_02375"
     CDS             complement(22053..24308)
                     /locus_tag="PLW08_02375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPL51480.1"
                     /translation="MKLTENALKVLEKRYFRKNENGVLIEDWDKMLTRVASNIASGDE
                     EKTNRFYQLMDAGYFLPNSPTLMNAGGDLQQLSACFVLPIEDSMESIFETVKNAALIH
                     KSGGGTGFSFSHLREANARVRSTNGVSSGPLSFLKVFNAATDAVKQGGTRRGANMAIL
                     NVDHPQILDFITCKENPAELTNFNISVGITEDFMQAVANDDEYNLISPHTGVIYKKLK
                     ARDVFALIVKMAHKNGEPGIVFLDRLNNANPTPALGKIESTNPCGEQPLLPYEACNLG
                     SINLALLIKDGDVDWDKLKEVVRESVDFLDAVIDVSKFPLPQIEKISQANRKIGLGVM
                     GWADLLFQLKIPCASEEAVALAAKLMEFIDFYAKERSSELAQEKGAFPNFQESIYATG
                     YLKREKINQPWDSLKEKIKKEGIRNATLTTIAPTGTISMIADTSSGIEPQFSLAYVKN
                     VMDGEKLIYVNKYLQKALEEKGIYNNKLLEEILTRGSLAGIEGIPDDLKKVFQTAHDI
                     SPEWHIRMQAAFQKYTDNAVSKTINFPNSATVEDIQSAYELAYHLGCKGVTVYRDGSR
                     ESQVLYLGTQTTSPEKTDKKVAPRTRPEITKGITQRLETGCGHMYVTINTDAQGACEV
                     FIQMGKVGGCASAQLEAIARLVSLALRSQIKIESITRQLKGIRCQSPMWNKGKMITSC
                     GDAVGQALEQFVTLYTKGQIQAGELSPENENPGSHQSVGNGFALCPDCGNTIEHTEGC
                     LKCPACGWSKC"
     gene            complement(24320..25150)
                     /locus_tag="PLW08_02380"
     CDS             complement(24320..25150)
                     /locus_tag="PLW08_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPL51481.1"
                     /translation="MEPLILTAAITGAETTRADQPNLPITPEEQAKEAKACFEAGARV
                     IHLHIREDDGRPSQRLDRFQEAISAIRKAVPEIIIQISTGGAVGESFDKRLAPLALKP
                     EMATLNAGTLNFGDDIFINHPADIIRLAEAFKQYNVVPEVEVYESGMVDAVARLIKKG
                     IITQNPLHIQFVLGVPGGMSGKPKNLMYMMEHLKEEIPTATWAVAGIGRWHIPTSLIA
                     MVTGGHIRCGFEDNIFYHKGVIAESNAQLVARLARIAKEIGRPLATPEQAREILALNK
                     "
     gene            complement(25354..26157)
                     /gene="murI"
                     /locus_tag="PLW08_02385"
     CDS             complement(25354..26157)
                     /gene="murI"
                     /locus_tag="PLW08_02385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPL51482.1"
                     /translation="MKRAIGIFDSGVGGLTVYKAIRNTFPNEDIIYFGDTARVPYGPK
                     SPNTIIDYSLQNARFLLQQGIKILIVACNTSSAVAIPALQNLSDIPVIGVIEPGAEQS
                     VKVTKCKRIGVIGTEGTIRSGAYPSAIRKLLPEAFVVSSACPLFVPLVEEGWQEHPVA
                     KVIAEEYLKEMKEQNIDTLVLGCTHYPLLSKTIQQVMGEEVTLVDSAMAVAEYLSRYL
                     SDENDGQKGRDSFYVSDNENKFAEIAERILQHNIGNLTRVHLYESWFKD"
     gene            complement(26154..28445)
                     /gene="rnr"
                     /locus_tag="PLW08_02390"
     CDS             complement(26154..28445)
                     /gene="rnr"
                     /locus_tag="PLW08_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPL51483.1"
                     /translation="MNYNELKQAILDYFQKYPHTGLSYNEIVNTFQLNKAEKALLSGI
                     LSGLMEEGILSKDRKKYHLQKMPKAKQPSPTPNPKLLQGIFDATPLSRGFSYAFIRTP
                     EKDYYVDAEDILNAFHNDIVAFEPKYRKGHQDSAIIRKVVKRSTEQFVGELIRRNGFS
                     TFICSNPKIFRWFFVSDPAQGENGDKVVLKVTNWGNPQMGKVPVGKVIEILGKSGEPE
                     VELLAVIRQYNLPLEFPEDVIAEANLLSAEIKPEDYRNRRDLRNLYSFTIDPITAKDF
                     DDAIAVEKTAKGWRLYVHIADVAHYLSVEGAIFQEASKRGNSFYFPKRVIPMLPERLS
                     NLICSLRPEEEKLCLTVQTDFDRKGQIIEQQIYESVICSQSRLNYDEVDDLFAGKETE
                     IPIPLQDILLEARTLSRLLTEKRMNAGYIFFDLPEIEYEYDENGFLKCLTLAEETESH
                     KIIENFMLVANEYVATRLSQTAPATIYRIHNDPDFNKLERLIELLSYYGVKWVMYENL
                     NKSIQYLLNSFPNEAYHKVFDRILLRSMKKARYSTEHIHHFALAMENYTHFTSPIRRL
                     CDLVVHYLCKTWLCHTANQKFTPSQLKHFAEVASEQEIQADQSERDIEMVYSLALMRE
                     KEGELYEGMVISVNSRGLIVQLEEIPVTGVIKEISLGEGNWEYLEREMRYINRRTHKY
                     YQLMDTLTVIIGYVEDDIYLFPIPETLRHNVSKTKSSLSHKSASQKPKPSGRMKSPSS
                     KRTGRTKSPSSKSYKSSTTRGNK"
     gene            complement(28454..28891)
                     /locus_tag="PLW08_02395"
     CDS             complement(28454..28891)
                     /locus_tag="PLW08_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51484.1"
                     /translation="MLPEPILKWKSWPLVERPWTSFILILFLILLSFLLYYITVTGWQ
                     QPFYYILGMLLVLGNLLPYFIMTEYELYEDKVQVRYLFLKISRPYTDFGCFYEDKRGI
                     MLSTFTMPRRLDPFRGLSLRFSADKQEREKLIEILMQKIGKKY"
     gene            complement(28881..29756)
                     /locus_tag="PLW08_02400"
     CDS             complement(28881..29756)
                     /locus_tag="PLW08_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPL51485.1"
                     /translation="MKSMTGFGNALISRDNIDLELEIKSINARFLDLRIFLPRELSFY
                     ETEIRRQIIKSISRGAIEVRVNFSDHREPKLVLDKTKLLKYNELAIEAAQLLASEEVI
                     SIEFLLQEPGVIESVDRLAEDEILANVLNEALAQALQRIKESMLEEGEQIKQVLCDSM
                     QQIEMAINAVSELCEPFKKELFATMHKHIEELIGSYKVENLEQRLIQELAIYVDKYDI
                     GEELSRLRAHLKTFTSTLQEEGDIGKTLNFIIQEMQREANTLGSKFSTTQSFPWILTI
                     KEEIEKCREIIQNVA"
     gene            complement(29998..31281)
                     /gene="miaB"
                     /locus_tag="PLW08_02405"
     CDS             complement(29998..31281)
                     /gene="miaB"
                     /locus_tag="PLW08_02405"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPL51486.1"
                     /translation="MKFYIETYGCQMNVADSELIASILTKAGHQEVHQISKADLLLFN
                     TCSVRGHAEERVLGRIQSENHRKKENPNLKIGVVGCMAQRLGEEINKDKLTVDFVIGV
                     DQYQHLTEILNEETEKTILTDLDETQLYKGIQPVYHNDYCAYITIMRGCNNFCSYCIV
                     PYVRGRERSRSWQDIIQETISAGKQGKKDITLLGQNVNSYLNGEVNFPSLLVKLNELD
                     EIYRLRFITSHPKDLSEELIKVLANSAKICEHIHLPVQSGSDNILKAMNRNYTVQHYI
                     SLVEKLHKFIPNIAITTDIMTGFPGETENDFQDTLSLMKTIEFDDAFCYKFSPRPGTV
                     AETLSNQVPEAERLARLQQMIELQRKISLKKYREQIGRKVEVYIESFSKKSRKMVLGK
                     TRDFKTAVLPGTEDDFGTLKQIEVKDATASTLICF"
     gene            complement(31278..>32373)
                     /locus_tag="PLW08_02410"
     CDS             complement(31278..>32373)
                     /locus_tag="PLW08_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51487.1"
                     /translation="NYPEEKALIAGLKNYTNCLQIDTLQLSAGQWKAIFEDIKPRKIR
                     TKTYKNCNTEVAGLVLKKYTQLQNRRLLSVFDLGSNNLNYVLFDLERRQIVHQTQLTT
                     ALGVAFKHNKISEKRLTSVKQIISKLLQLDSYLESEKVLLATGVMRFALNANLLTDWI
                     TDKYNIKCRIISSQEEIRYAYFSARQYKSGEEATLAIDIGGFSTEFINLDKEKKQQGN
                     SLPFGLLTLLNDFNGDSETADNFIRQKLNDLPYKGSYCLVGIGLTYTYLAGVIFRCGY
                     NEREKMEGKTITKKQLQELSHSLEVRDEQRYLPFLLEESYLPILKLSIAFNISLLDRF
                     ETSEIIVCNSGIAVGYAYWYSSKQRSKAQK"
     assembly_gap    32374..32473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32474..33186)
                     /locus_tag="PLW08_02415"
     CDS             complement(<32474..33186)
                     /locus_tag="PLW08_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51488.1"
                     /translation="MLKFALYVSNHGFGHATRICALAEELIHYGVFCHIITTKPAFLF
                     QDLNPHYFVLHNRAIDVGVKHSENLTVDIKRTINSLLELLSKRNEIMEREIEFLRREK
                     IDCIIADIPFLVSDFAAYCHIPVFAVTNFEWHFIYSSLLAKEENTEKENGIVNNYNNL
                     ELSGGAASSGYTNFSTPEVANSLKPVLNLIWSLYQRFDGCFRLPFSTDESMSAFKDYI
                     SCGLLSRKKDKYKDIRLQY"
     gene            complement(33196..33564)
                     /locus_tag="PLW08_02420"
     CDS             complement(33196..33564)
                     /locus_tag="PLW08_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPL51489.1"
                     /translation="MKKYSILNDLKPADVVLILLIILAIFLSARYYLSNKPHSQVYIY
                     KDNQLFGVYSLEQNKVIKIDEHNTVEIKNGKVRMKFADCPDKRCVKQGFTKSMPIICL
                     PNKLVIEIQDNGKQKKLILQ"
     gene            complement(33565..34428)
                     /gene="amrS"
                     /locus_tag="PLW08_02425"
     CDS             complement(33565..34428)
                     /gene="amrS"
                     /locus_tag="PLW08_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPL51490.1"
                     /translation="MREAMYYVREGNKIRCQLCPRECVLSEGKKGFCRSREVINGSLI
                     ATAYGRTTSLALDPIEKKPLYHFHPGTMIVSLGPNSCNLTCKFCQNWEISQQEYPTRY
                     VAIEDLIEIVKQQKDQQVAFTYTEPLMWYEYILDFSAKAPEIDIVLVTNAYINKEPWR
                     NILKVVKAVNIDIKSYRDEFYRQLCGGKLDIILDNIIIAKEMGVHIELTNLIIPGYNN
                     SEEELNDLAKFIASVDKNIPLHISAYRPCYKMTVRPTTREEVEHACEIASQYLTYVYA
                     GNVYSSRFSRR"
     gene            complement(34428..35570)
                     /locus_tag="PLW08_02430"
     CDS             complement(34428..35570)
                     /locus_tag="PLW08_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPL51491.1"
                     /translation="MASIKAEGEDIHKGILFHSASMGELTAIKALVTKHLQEHPEVKL
                     CITTSTVTSCAEANKISPKVKGFLSVLDLPHLRNRQLKRINPGLICIVETEIWPNMLL
                     WAKRNKVPVLFLNARMSKSTLRGYRLLKFLFRHLQSPITEIHAQSKDDAKRYRKIFNR
                     PVYVSGNLKFSLTLKDYNAEELRKKWGYKSDDFIICWGSSRPGEEALLISLYPSLKEV
                     IPNLHLIIGLRHPRRLEEVIELMDNYSYSCFTMRKEYSRAEDILIIDTLGILDQAYCI
                     CDIAIVGGSFYDFGGHNPLEPAYYKKPVVIGPHHYSCKESVKKLKVAGGIIISNKDDL
                     LADLIALYKNKELREKMGKAAKKVLTENAHTLDIYLKALEKWLKEE"
     gene            complement(35710..35787)
                     /locus_tag="PLW08_02435"
     tRNA            complement(35710..35787)
                     /locus_tag="PLW08_02435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35750..35752),aa:Asp,seq:gtc)
     gene            complement(35916..37451)
                     /gene="guaA"
                     /locus_tag="PLW08_02440"
     CDS             complement(35916..37451)
                     /gene="guaA"
                     /locus_tag="PLW08_02440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPL51492.1"
                     /translation="MHNKVLILDFGSQYTQLIARKVRSLNIYCEIHPYQISLKQIQSF
                     APQAIILSGSPSSLSKINSPKPDEGIWQLRIPILGICYGMQLLVTHYGGTILPSNRSE
                     YGSAVLNIDNHSELLAGLEDREEVWMSHSDAVSSLPECFSVLGKTDQSPYAVIAHKEL
                     PFYGLQFHPEVANTKNGIKVLENFLIRIGKITADWTPNAFINESIEKIKEKIGNQKVI
                     LGLSGGVDSSVTAALLYKAIGNQLLPIFVDTGLMRYREAERVKESFSLYPGLNIRYVD
                     AGRTFISKLKGITDPETKRKIIGNTFIEVFEQVAKDYEDVHFLAQGTLYPDVIESTSA
                     AGISSTIKSHHNVGGLPEKMNLALIEPLRELFKDEVREVGTLLGLPESIIQRHPFPGP
                     GLAVRIIGEVEEEKVHLLQLADEIFISELQKNGLYNTTWQAFAVLLPIRTVGVMGDER
                     SYDQVCALRAVDSVDGMTANFTQLPMWFLQKVANRICNEVKGISRVVYDISSKPPATI
                     EWE"
     gene            complement(37460..38086)
                     /locus_tag="PLW08_02445"
     CDS             complement(37460..38086)
                     /locus_tag="PLW08_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL51493.1"
                     /translation="MIKIESTVLLESFQTNTYLLWESISAEAFLIDPAFQSETFLQRI
                     KQLELKVKMIINTHGHADHIGGNLYFAKALNCPVAIHLADAEMLTNNTKNFSTYMGFE
                     LNLSAPQVILHDGDELFLGSEKVKVIHTPGHTKGSICLLAGKYLISGDTLFEQSIGRT
                     DFPGGSHTAIIDSIKNKLFILDDDILVFPGHGPTTSIGLEKVNNPFVN"
     gene            complement(38102..39097)
                     /locus_tag="PLW08_02450"
     CDS             complement(38102..39097)
                     /locus_tag="PLW08_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HPL51494.1"
                     /translation="MKTHKAIALFSGGLDSILAVKWMQSKGYAVHPVFFKAPYLLPER
                     AINYAAKNAIDLEVIDVSEEHIKLLDNPVYGFGKGLNPCIDCHGFMFKKAAELMPDKD
                     ADYLISGEVLGQRPKSQKRNALNNVSKLSGVKDLIVRPLSQNLLPDTLPLREGWIDKK
                     DMLSIQGRGRLAQLALAEELGIIDFPSPAGGCLLTDRNYCLRLQDLKKHQQDDLHSIN
                     LLAYGRHFRLTEEVKLIIGRDEAENETLKNIFQEGLMLQAQDYMGPLGFITGKLNFAT
                     LDLALAIFILYHPKVPKSANIEVWRISNKQIIGQMIKTATKGDREIINKYLISYS"
BASE COUNT        12179 a         7546 c         7021 g        12197 t
ORIGIN      
        1 tttaggtttg ttttacacat tcttcacaga gatgataacg acaaaagcgg taataaatat
       61 ctatcagtcc ttgctggtaa agggtcttct tattaacaat gctaacttgg ctggaattga
      121 tatgattaca cataaagcgg gtaatataat tttcgggtaa tgccttaaaa gaaagatagg
      181 attctaaaac ggattcagtc atttccgtat ttgccagttt ttgatagtaa agatagagaa
      241 tgggcagata aatattgatg taaatattgt tgataaccgt aatacctaag ccagtattat
      301 ttttcaacag tccttcctgc cctggtttaa attgtttaca aaaggtctga tatctttttt
      361 ggggattggg tttggctaat tctactgtct tgagaaagaa gtgcaatacc ccttcctgta
      421 aacagttata aagaaattca cttaatagaa taagacgaaa aacaggatga tttcccggac
      481 ggatacgaaa aagttgccag tccacattaa ttttacgcgc ataaaatctc tggctttcaa
      541 aagagcggga aagggttgca taaagttcgc cgtttaaacg attccgactt ttcagtaata
      601 aaccggaaga accaagaaaa atactgatca tttcatcact tttcagccct tctttgtgcc
      661 actctttcaa tttggcaaag ggaatgcttt gtgccagttg aaaaagattg aatttattct
      721 tgtcgtatcc tgctgcttcc atcattcctt catagaggat ttggtcaaaa tcgcttaaag
      781 ccaaagaggc attaaatcgg cgcactttac tcataaatcg ttgttttccg gaaatggtta
      841 aaatgcttag taatctatca ttatcaatgg cagaaagaag atcgcaataa tcactgccac
      901 tggctaagtt cttatcgcca atatcagcca gcagtttttg aatttcatcg gaaagctgat
      961 ttttcaattc cagaatttct accagttcac cattttcttt catagtaaaa ggtaaagggc
     1021 tatcattatg caagacaata tgtaaaataa cctgattgta aaacacatct tcctgatgag
     1081 agtgtttaat ccaatcctga gtgtttttat gaatttccac cgctccctgt aaatcctctc
     1141 catcaataga tattatggca ttaataaaat cgggaccccg aaaagtattg aattgaccct
     1201 ggtaatttac tttcaggtct ttaccgctaa ccgttttcag gtttggagcc aagtgaccct
     1261 catcccagat atgataaaga aatttttcat ccattagaaa tacctctgca gcaagttata
     1321 tctttgcctt gcggaagctg atttataata tgtttcagga gtatagcgaa gaggattggt
     1381 taaaatgctg atcaatttca ttgtttgcgt ccgggttaaa ttcctacagc ttttgccata
     1441 gtaataacgc gatgcagttt caatgccata aattccctta ccccattcaa cataattgaa
     1501 atagagctcc agcattctct ttttactcat acaaatttcc ataatcaccg ttgcctgaat
     1561 ttccaagtat ttacgcagat aactcctatt tgtggttaag aaaatagttc tggcaagttg
     1621 attactgata gtgctggctc caaaacgaat ttttccggct tttttattac gctgatatgc
     1681 ttctttcacc attttccatt caaagccgaa atgcttgtaa aaattggcat cctcaatagc
     1741 aataagcata ctttgggttc tttcaggaat tttttccagg ggaacataat accttttatg
     1801 aacaggataa ccacgcacca gatatctctg caacattaaa ggtgtgacag gaggattaac
     1861 aaaattatac aaaagtgaaa aaaatgctat tatcacccaa tagcaaagat ggataataac
     1921 aataaaccta ataatacgca gccagatgga acgcctgcgt ttcaagaatg atccttattg
     1981 cctaaaacta ttttgcttgt ttataatgcc tgaacactaa ttcaagatgt cttttctacc
     2041 ttagctttat taacataatc agctaaacga gattttctgc gagaagccgt tcttttgtga
     2101 ataacacctt tcttgacagc tttatcaagt ttggaataga gttcagaaag cagtttttcc
     2161 ttttcttcag gagtggtatc gctatgaatt tttttggaaa gagttctaat agtgtttctt
     2221 acataattat tgcgagcagc cctctttttg tctgttttca ttcttttcag cggggacttg
     2281 tgttgtggca taatatcctc tcttttttta atataagtat ttatttggca cacataaaaa
     2341 tgcccttcat ttctgtcaag aaaattgtga ttggaaatta gagtgaaaaa gcgaaatagc
     2401 gatatggatt ttactctcta tagaatatct cctgttttcc ctttttaccg ctgttacaaa
     2461 ttttttcagg tttaattgtc atcttagaga aaaaagggat tgacggaaac agcttataaa
     2521 aaattgtgac ttaccaatat aaaacaatat acaaaaaata tatgataaac gaactgagga
     2581 aaagatgaaa gacaatgaaa tgaagaagat tcgtaatcta ttgttcattg gagccagtgg
     2641 tgcaggaaaa acaaccctgg cagaacagat attttactta acgaaaacga caaaccgctt
     2701 gggcagaatt gaagaaggca atactataat ggattttgat cctgaagaaa tcgctaaaaa
     2761 tatgtccttg ggtctttcca ttggttatgt taaatatctt gatcataaga taaacattct
     2821 ggatacacct ggttgtccag atttcgttgg agatgcaatt gtagctatac ctgctgtgga
     2881 aaatgctata atcgtagcta atgcggcagg tggctatgaa gttggtttgg aacttgcctt
     2941 ggaacaaatt gaagaacgca aaatgggtaa ggtgtttgta gttaaccgaa tggataatga
     3001 taatgccgat tacgataaaa ctttggaagc aattgctgaa aataccgata ttaagccggt
     3061 taaaatttat cttcccattg gcaaagaagg tgcttttgaa ggtgttgtag atattattaa
     3121 acgaaaagca attaaagccg gaaaggaaag tgaagttcca accaatttgg cggaagcagt
     3181 ggaagaagca agaattcatt taatggaagc tgttgccgaa accgatgagg atttgctgaa
     3241 cgaatatctg gaaaatatgg aactgccaga agataaactt attgaaggac ttaagaaagg
     3301 aatcttaagc ggtgaaatat ctcctgcctt tgtttgttct gccggaaccg gagaaggagt
     3361 tttggctctt ctgaacggta ttataaacta tcttccttct cctgccgaag caaatgaaat
     3421 agaagttctt aaggaaaatg agaaaaccgc ttttatttgc caacccgatg gtgaactttt
     3481 gggttatata ttcaaactct atgccgatcc gggaatgggg gattttgttt atttccgtct
     3541 tttttccgga acactaaaga caggtatgga attttacatt cccgaaaaag atgccaaaga
     3601 taaagctgga acaatgtatt ttatgttggg gaaaaatcgt aatgactgca acgaaattaa
     3661 agcaggcgat attggcgctt tactgaaact aaaaaacgcc aaagttatga acagcattgt
     3721 ttctcttggt tctaaaatat ctgtaatccc ggtagaatta cctaccccaa ctacctggca
     3781 aaccatcaaa gctgttaatc aagccgatga agataaaata ggcactgtct tgcaaagagt
     3841 gattgctgaa gacccaacca ttcgctatga attaaatgtg gaaacccatg aaaatgttct
     3901 atccggaatg ggagaacagc aattgcaact ggtgctgaag aaactgaaaa accgttataa
     3961 agtagatgcc atcctcaaag aaccgcggat tccctataaa gaaacgataa tgacaagcgc
     4021 agaatccaac tataaacata aaaaacagtc aggtggccgc ggtcaatatg ctgaggtcta
     4081 tttccgtatt aaaccaacag aaaggggaga aggattcaaa tttatcaatt ccattgtagg
     4141 cggagtaatt ccctctaatt atattcctgc aattgagaag ggactttggg aaacaatgga
     4201 aaagggtatc attgccggct accctgttgt tgatatttcc gtggaagttt attatggcag
     4261 ttatcacgaa gttgatagct cagaaattgc ttttaaaata gcttcctcaa tggctttgaa
     4321 ggatgctttc aaaaaagcca aacctattct tttggaaccc attcatgaat tgattataat
     4381 cgttccgagt gaatatatgg gtgatgttat gggtgatatt tctacccgga gaggtaaaat
     4441 tctgggtatg gaacaacaca gtaaaaaaca atatttaaca gctcaaatgc ctttagcgga
     4501 aatgtattct tactatccag ccctgaaatc attcacccaa ggtagaggac gttttatcca
     4561 aaaattctca cattatgaaa aagtgccgga tgatattgct cagaaagtga tttctgcctg
     4621 gcaggaacaa accaacgagt agggtgaact gtaaaaaggg gaaaccagag attgaataac
     4681 agaagaatga tgttctcatc gttcatttat aactaatctc tcgctttatt gcaaactaag
     4741 aaagcccaat gtttcttact cgtggttgat gaggataaca accttcctaa atctgtgaac
     4801 cacgattgat tgtgtataca gaggaagccg ttcctccgta aaaaaattca gagaaatggc
     4861 gagctccgtt tccacttttc ctttttttct tgacaataaa ggactgcttt tcataatgat
     4921 tttgtaagat gataaagaac aaattgaggt ttatatgtct ggacacaata aatggagttc
     4981 aataaagcac aaaaaaggtg ctgctgatgc taaaagaggg caggtcttta cccgcttggt
     5041 taaagaaatt attttagcgg ctaaaaccgg aggtggagat cctgatatga atccccgctt
     5101 acgaaccgct attaatactg ccaaagaaaa caatatgccc aaagaaaata ttgaacgtgc
     5161 aattaaaagg ggaacaggtg aaattgaggg tgccgcttat gaagaagtta tttatgaggg
     5221 ctatggtcat aatggagtag gaattgttgt agaagtgatg acagacaata aaaaacgcag
     5281 tgtttctgaa cttaggcata cattttctaa atatggaggc aaccttgcag aaacgggttc
     5341 ggttgcctgg aattttgaac agaagggatt tttcaatgtt ccttctgcag gtttggatga
     5401 agatgaattt atgcttaaag cactggaagc gggagccgac gatgttgaac ttaatgatga
     5461 taattttgat gtttatactt ctcctgctga ttttcatatt gttatcggta atctggaaaa
     5521 agagggctat cctatccata atgcagaact taccagagtg cccaaaaata caattgccgt
     5581 tgatgatgat actgcagaaa aactaatgcg cttgattgaa tccctggaag agcttgatga
     5641 tgtgcaaaag gtctatgcca attttgaggt ttcagacgaa gttatggaga aactttctca
     5701 ggcataaata actaagtgat tattattggt tttgatcccg gcagccgtta ttgcggttat
     5761 ggtttacttc aaatagaaag cagaaaagtg cttgctgccg gctgtgatgt tattgatgtt
     5821 acgcggggaa aaacattggc attaaggtta aaatcactct atgaaaacat tatgcaaatt
     5881 ctggaagaat accatcctga aattgcagct gtggaaagca ttttcttcca tcggcaggtg
     5941 agaagcgtat ttactttagg tcattcacgc ggtgttattt tacttgcttt agcaatgaca
     6001 gatattcctg ttgtggaata cagtccccgg gaaataaaaa aatctgtttg cggcaacggc
     6061 aatgccagta aacaacaagt tcgctatatg ataaatcagc tttatccatt aaaagaaagc
     6121 cctaaaaggg atgatgcttt tgatgcctta gccgtagcaa cctgtcatta taaccggatg
     6181 aagtggttac aatgattcag tatatttccg gaactcttac ctttaaaaca cctgttttag
     6241 ctattataga aacaatgggc atcggctggg aactgaaaat accgattagc acttatgaaa
     6301 aattgccaac tgctggtaaa ccctgtaaat tgcttactta tttacatatc agtcaggatg
     6361 atatacgcat ttttggcttt gctacggaag cagaaagaga actttttgtc atgttgaaca
     6421 aaatcggcgg aattggtcct aaaattgccc tatcaatact ttccacgcta agcattccta
     6481 cttttataaa agctgtcaat tctggagaag aaggtctatt gacaaaagtt ccgggcattg
     6541 ggaaaaaatc tgctcagcgt ttgattgtag aattgaaaga tgatgtccat aaattactta
     6601 accatataga ccagaaggaa ttgcaaatag gggatgttag cagtgaagtg gaatcggcac
     6661 ttttgtcttt gggatttaat ttgcatcaaa tccgtaaaga actggcttta atggatgagc
     6721 caatgcaaaa actaaatacc gaagaattaa ttaaagaaat tattaaacgc atctatcaga
     6781 gaaataaatg aatttccgct ccgaagcata tacaactatt ctgaaagtgc ttaaagataa
     6841 agaatattct gatactttat tgcatcagag agccaagaaa ttaaaaaatg caggagaaga
     6901 ccctgctctc ttttacaacc ttgtgaaagg ggtgatcaaa ctctatctga aactggatta
     6961 tatccttcag caatatgtag ataagaagaa atacaccgac accgatatta agattaaagt
     7021 cctgctctat atggggcttt atcagctttt gtatctaaat tccattccag attatgctgc
     7081 agtaaatgag accgtggaac tggctaaaac cttattcaat cctcaagtgg cagattttgt
     7141 taacgctgtg ctcagaaatt atttgcgcaa tccggaagtt tcttatcctg aggaacctgc
     7201 tttgcgtatt gcttatgaac attcttttcc accggaattg attaaacagt ggttggattt
     7261 atggggtgag gaaaatacgg aatacttggc tatgtttttt aatgaaaatc cggaactgca
     7321 tattagagta aacagcactg ctacaactcc tgaaaagttg aaagcatatt ttgccaaacg
     7381 caatattgta atcactcctt ctcaagcgag tccgatgatg tttcatacag ctcaaacagc
     7441 cgaagttttg gaagatgtag cattctccga aggatatttt tccgtgcagg atacttctgc
     7501 cgcactaatt gtagaattgc ttgatcccca aaaagaggaa agtgttttag acctttttgc
     7561 tgctccaggt ggaaaatgca gctatattgc cgaaagaatg gataatacag gtgaagttat
     7621 tgctgtggat aaaattccca gtaagatgaa actgttgaaa caggcagcaa accgtttaca
     7681 gctaactaat atcaaaatgg ttgtaacaga tgcctttaaa tacggtcccg tggctcctgc
     7741 ttttcacagg gttttagtag atgtaccttg ttccggatgg ggtgtttttg gaagaaaatc
     7801 agacctccgc tggcaggcac atcaaaatat ccccgagctt ataaaacttc aggaaaaagc
     7861 attggattat gcagccaatt ttgttgctcc gggtggtttt ttggtttatt ctacctgcac
     7921 aatgaatccc aaagaaaacg aagaccaaat ttccgccttt ttaaaaaaga acaggaattt
     7981 tgaactgatt ccagcagatg catttattcc ggaaagatat acctacgccg gatacttgaa
     8041 aacgatacct ttcaaacata atatggatgg tgcttttgca gccaaattac aacgccggaa
     8101 ataaaaatat tattgctcgg tcaaaatccc caacgggcac taaaaaagcc ggttatataa
     8161 ttggaatcgg aattggaatt ataatcgtaa cagcttttgt tttcagccag attctctttc
     8221 ctgttatttt aggaagaact cccaaagtag aagttccttc cgtagtagga atgacccttg
     8281 ttcaggcaaa aagagtactt caggaagaaa aattgcatgt aatagttaag gactcttttt
     8341 ttagcgatgt tgcagctgcg gaagttgttc tggaacagaa tcctgttgca ggagaaaaaa
     8401 tacgccagga tggaacagtt tatctggtta tcagtaaagg ttcttctaca gtaactttgc
     8461 cttcattgat tggtaaaccc tttcaagagg catttataat tttaagaaat ttggatttgt
     8521 atagcagtgt ggtggattcc acctattccg atatttatcc ggttaacact gttatcagaa
     8581 gtattcctgc tgccggggaa aaagctttga aaaaatctac tgttaagcta attttaagca
     8641 gaggaccgga accggtagca gatacactta attatgagat accaatatat tattattaaa
     8701 aacttaggat gggaaatgca caatgaaaga aacacttaaa gaagcaaaaa ttcgctcgcg
     8761 tatgatgcca ggagcactta ctataacagg ttttctggga acagatcaac gccctcttgc
     8821 agaaattatt gctgcagatg aacaagaact aaaaaaactt aatcgcagtg tggaagaaat
     8881 tgcagaaagg atgaaatatt tctccaatgc aacttttgat gcatataaca acacagtcgt
     8941 tattgatgac acctatgaag tacatactga atcaaccaaa ggaacaatga cctgccctta
     9001 tggacatggg ggactttaca ggaagtatgt aacaacatta acaaatcgca aaaataacct
     9061 tactgttaga tggaatgcta ttgatatcca tctaattgaa gctcatcatt tctttgaagg
     9121 gaaaggttca ccgtttcgct tagaaccgga aattctggtt aaagcgttat tttcctaatc
     9181 ccgacaaatg tttagctgga agccactgtt tctctgcaaa taaaaaaagc ggagttacga
     9241 gagctccggc tacacgattc cggaaaaacg atgtcctcgt cgttttatat aaagtcgtaa
     9301 tttgtagtta atggggacat caactttcca gaaaaatgtt tacttgattg tttacatcta
     9361 cgaaagagat atctcctttt gtgcgtaagt tatctccttc taaaaatttg ttatataacc
     9421 ccaaaatata atttgctatt tcttcactat ctttgctaac gaatccgctt tctgttttac
     9481 agattaatgt atatacttca ccagccggtt cggaaataca aaggatcgga gttcgtgaag
     9541 ccatatattc aaaaagtttg gagggtagta aaccttcttt gcttttacag gtatcaataa
     9601 tcagtataag cagttcagca ttcaccaatt cctctaaagc tttgctatgt ggtaaaaaag
     9661 gaatttttat taccgggaaa tcagtttcgg gaaaatccct ggtgccgata agagagaatt
     9721 ccagcaaagg tagttgcaac gaagaaagtg ctttcattaa agggctggca tcccgtccgg
     9781 ttgtaagttg acctacatat ttgatgcgaa atttatctgt tcttgagtaa tgcaaaccct
     9841 taaaatcatc agggtcaaag ccattatata agaccttctt tttgccatcg ggtaaagcat
     9901 ctgcaattac tttactgaat atataattta ggtcagctgt ccggataatt ttccgttcca
     9961 gatatttatg caaagccaaa gttatacggc agggaggatt tattttcaga tagtagattt
    10021 ccgacatagg gtcccgaaaa tccgttctcc actcaatacc aaaatgtttt ttcagtttca
    10081 tacctactaa atgtgtagag tggggaggac ctgtagtaat aacatagtca attttatcct
    10141 ggcgtaaaac ctgcagtgca tttttataag cagaaggatt ccagccgatg cgcatatctg
    10201 gaacaataaa attcaagcgc agccaatata aaatcttttt taaggaactg tcgcttgctt
    10261 tgttttggag tgaaccgtaa ggtagttctt tttgtcctaa tatattccac aactttccga
    10321 atcttaatgg tttactgcga taaaccttta ccgaagaagg aattttagcc agtaagcttt
    10381 catccaggta aggataatca ccatttagag tagttactac ataaatatct accccttttt
    10441 tagttaaagc acgaataaaa cgcagccatc tttgaacaga ggcacctccg caaggaggaa
    10501 agtaataagt gataagtaaa atcttcattt gatcagtaat tccgccaaac ctttccagga
    10561 ataacgcttt gtgtattcat taattgcctg gctcatatcc ggtttttgtt gcagatggtt
    10621 aattattgcc tcagccaaag ccgaaggatt atcaggaggg acaagataac cagttttacc
    10681 ttcaataaca tattcaccca atccaccaac cttagttgcc agaacaggaa ctccaaaact
    10741 gtaggaagtg gctattacac cactttgtga agcactttta tagggtagaa tacagagaca
    10801 ggactgattg aaaaaatatg atatttccgg atcgccaagg taacgaaaat agcattccac
    10861 attattatca atacctaatg cctgaatctg ttcaatgtag atatttttat ttccatagac
    10921 ctctccggca attattaact tcacatcagg aaacgcttca atcactttgg gcattgcttg
    10981 cagtaaaata tcaagacctt tatagggctt aattagaccg aaaaaaaaca gggtattatc
    11041 tttccgggta gccgatatat tgtcattata catatcatag cagggatgaa aacctgaaac
    11101 acctttttta gatatataca gcggtaattt acgatgcagt tcatccagag tagctttact
    11161 taaggtaaca attttagttg ccggcttaaa agcatattgc agaaaatatt tttcgcccaa
    11221 ccatttctcg tgagaggcaa tattatgtgc cagaattact ttacgggaac aggttatttg
    11281 tcttaaaata taaccataag caggagcaaa atagggtaaa aaccaggaga caataattgc
    11341 ctcgggtcta tatattttga gctcgtgaat ggttgccttc caggttttag gtaaccaggg
    11401 agtaaggatt tgatgattaa caaattcatt ggcaggaagt tctgaatcat attgtccctt
    11461 tccagggaag agcaacttgg gatattgctc tctaaaattg aaaaattgaa cattatgtcc
    11521 tgtatcttta aaagctctgg caagggataa agcgaaacga gaaataccgc cacggaaagg
    11581 aggtgcagga cccaaaatag caattttcat agcaaactct gttggaaaaa gcgctttaca
    11641 atatcagcat cctgatattt accgaaaagt gccatcaatt tcaagcgtgc tttaacccct
    11701 ttcatatccc cggcaagaat gcaacctatt gattccaaat gttttcctcc accctcataa
    11761 ccatattcag gcagaaccct tccggtataa gttctggaag ttattatcac taaaacattt
    11821 ctggctatag cttcttcaat atcgggaacc agagttttag gtaaatttcc tcttcccaat
    11881 gcttcaatca caattgcttt tatatcgtgt tccagacaac agcgaataaa ttttccatcc
    11941 attccggcta ctgctttgat aagttcaact cttgtatcca attgttccgt ccaaacattt
    12001 tcccgataga gagaagcacg atgatataca attttatcgg ggtctacatt gccaataggt
    12061 ccataaccag gagaaacgaa ggcatcaatt ttacccgtat cggctttcac gacatcgcga
    12121 gctgtgtgaa tttcatcatt cattactacc aaaacacctt tatcaattga ttccggatga
    12181 ctagctacgc gaacactgcc aattatgtta cgaggaccat ccaaaccgat ttcatcaccg
    12241 gaacgcattg ctgcagtaaa aataacaggt ttgcgggtag ttaaaactaa gtcgcataaa
    12301 aaagccgttt cttccaaagt atcggttccg tgagtgataa ctacaccatc atagtcaatt
    12361 attttcaggt ctattaattc agccagttca aacatcattt cgggagtcat ataaggactg
    12421 ggaatattca gatagtccat cacttctaca ttggcaacac tgtttaattg gggaaattgc
    12481 cttaaaaatt cagcaaattc ggggctggga attactccgg caggatcaga tgctttcata
    12541 gcaatcgttc caccggttaa gataatgagg atgttttttt tcataatacc taattttccg
    12601 tttatatcat ttcttgaagg aaaagtcctt tgtcattcct gagaaagcag gaatccacca
    12661 aagagaaaag aatattatac attttcaacc cgaaattaat gtcagttacg atagggaaga
    12721 gggtcttcca cacctgctgt tttaaatgcc tttttgcgta agagacagct ttcacaaacg
    12781 ccacaggcaa tatcattttc ggtataacag ctccaggata attcaaaagg agctttaagt
    12841 tcaattccca gctgcactat ttctgccttg cttttatgta aaacaggggt gctaatctct
    12901 atcggaattt caccttttgt tcctgtagca atcactttat tgaatgcctg aaaaaaaacc
    12961 tcacggcaat ccggataacc ggaactgtct tcttccacag ctccaatata aatttttgtc
    13021 gcttgaataa cttctgccca ggcaactgca gaacaaagaa atatggcatt ccggaaagga
    13081 acataagtaa caggaatttt agaaccggaa aggattcctt tctgaacgag aatgtttcta
    13141 tcggttaaag cagagccgcc aatctcagaa agccaggaat aattaatcac tttggcttga
    13201 attggctgat aataatttac cagtgcttca aaagaggata attccttttt ttccgttaac
    13261 tgaccataat taaaatgcag aaaacagacc tcgtcacatt cagatacagc acaagcagct
    13321 gtaaccaaag aatccattcc tccgcttaat aaaacgattg cccgggtcat aaactttatc
    13381 ctgattagta atttttacaa ttttatgtta ataatgcttt atgtcaagtt aagaatgaat
    13441 tttcacccgg tcatctgtta acttgttaac cttaatagtg taaatctctt attccttctt
    13501 tcatttttgc cagattttct ttcactgagc tctgcatatt aggatcatct attttagcta
    13561 cttctttgtc aatcagttcc aagccgattt ttttagtgcg ttcgctggca taatcataaa
    13621 gatattccgc aaaagtgagt aaggcattgg gagtgcaaaa tctttttaca aaaccaggaa
    13681 tggcaaattc cataaaatgt tctccagttc ttcctgcacg gtaacaggaa gtgcaaaaag
    13741 aaggtatata accgctatgg gcaagttcat aaataacatc atccaaagtg cgggtgtcac
    13801 ctaaaacgaa ttgactttta cgtttggatt cttcatcttt atgagcatag tcaccaactc
    13861 cgatactgct tccggcatca atttgtgaaa tgccatagcg taagacctca tctcttactg
    13921 taattggttc tctggcagta aggatcattc cggtgtaggg tacacttaag cggatgctgg
    13981 ctattaaaag tttaaagtcc tcatcggaaa ccttgtaggg agggtttttt gtaaagtccg
    14041 ttccaatggc aggttcaata cggggaaagg aaatagtgtg aggtcctact ccaaagcgtt
    14101 cttccagatg aattgtatga tagagaagac ccataacttc aaaacgccaa ttatataatc
    14161 ccattaaagc accaataccc aagtcatcaa ttcctgcctg catagctcta tccagaccat
    14221 ataaacgcca aaggaaatta cttttaggtc ctctgggatg caaatttttg taggtctctt
    14281 cataataggt ttcctgaaaa atttgatagg tgccaattcc aacggatttt acagtgcgaa
    14341 aaccttcaat atccaaagga gcggcattta tatttactct gcgaatttct cctttattgt
    14401 gttttacatt ataaacggtt tgcaccgttt cagcaatgaa ctcgggagaa tatttggggt
    14461 gttcaccgta aaccataatt aatcgtttat gccctttatc ttcaagagct tctgtttcgg
    14521 caatcaattc ttctttactt aaagttgccc gctgacattc tttattggaa atacggaatc
    14581 cgcaataaac acaatcgttt atacattcgt ttccaacata aagaggagca aaaagaacta
    14641 tccgatttcc ataaattcgt tctttgagag tatgcgcacc ttccagaata agatttttta
    14701 attcgggatc ggtaacatta attaaagcgg ctgtttcatc aggattaaga cgctgtttat
    14761 ccagcgattt atctacaata tctctaatcc taatggcatc ggcaaaacgg tttttgttta
    14821 aatgttcttc aatttcggca gtaggaataa aggacttaag accttcagta ttaattttca
    14881 ttttgttctc cttcctccag tttcaaagtt gtaaccttta ctttcacgga ttttatctgc
    14941 cccagttttc cggaaaaggc acccatttcg gaaacagaga gctctacaat taaaaaaatt
    15001 acagcaactc ctaaatcgcg cataggatag cccacccgta aaagaatatt ttgtgcataa
    15061 ctatgcaata attcgtttac gggatgaaaa gcactttcac ggtcttccat aataatgctt
    15121 agaatatgat atttggcatt cattttttcc ttccttcagc gaggagaaat tttagatggc
    15181 agacaaaaga ggaaaagagg tcttttcacc cttgatgcca gaacttatct ctcatcgcag
    15241 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggaagttc cggaagaaaa
    15361 gaggggtatc cccggcataa ggaactgtaa ttaatttcca gagctatttt gcggtctatc
    15421 atagtgttaa aaatatcttt cagcaaagga taaaaggcac tttcatcagg aatataatta
    15481 taaccccttt tataaacccc caaatgagca agaacatcaa tattacaatg ggaaaccagc
    15541 aataaatttt gctggtaata atccctaacc tgttcctcgg aaagaggttt tttaagcgga
    15601 acagcaacat ttgtagcgtc acttaaaaaa tgaacagaac ccaagataag gtcaaagtta
    15661 atatcttcaa ccaaagagag ggcatattct ttaaccctat gatagtcacc aacttcaatt
    15721 ccacaaagca cattaagttc tgatgtattg ttctgtaatt cttttattcg cttataataa
    15781 cggtctaatg aaggtgcacc gtatttcttt atttcctgag gtagtaaatc caggtgttca
    15841 gtaatagcta tttcatcgta attaagttga ttagctcttt taattaaatc atttgcttta
    15901 attgagctat cgtagctatc ttcagtatga atgtgataat caaatttcat ttggctaaaa
    15961 tggcagcgcc caaggcagcg gtaatttccg gttcaggagg ggtaagtaat tgagaattta
    16021 gtgcttcact taaacatttt cccatatctt tattcagggc aactccaccg gtaaaaacaa
    16081 gtggttgctc ccaaaccata acagacattt gggctaaaat gcgttttgct atgctacgat
    16141 gaacagagcg aactatatct tctggaggaa tagaagaaga aagcatcccg attatttccg
    16201 attcggcaaa aacaacacag gtggaattaa gcgtaagatt ctgagtggat ttggaagcta
    16261 aaagagaaag ttcggaaaca tcacaaccca aacgcatagc tgtcatttcc aaaaatcttc
    16321 cggttcctgc cgcacattta tcgttcatca caaaatccgt tactttacct tttttgttca
    16381 gggtgataat tttagaatcc tgacctccga tatcaatgat agttctaata tgaggaaaat
    16441 aatacagaca accggctgta tgacagctaa tttccgataa aatggagctt gcttcctggt
    16501 aaagtttgcg cccatagccg gtaaccccaa tagtgtttat ttttcgggta attcctaaag
    16561 ccgtataacc ttcttttaaa aggttgttaa ctccatttgt aacagaaact tcagtggttt
    16621 gaaaagcact aaattctatg cgttttgttt gggcatcgta gataacgatt tttgtgtttc
    16681 tggagccgat atcaatacca attttcaaca taattacaac aatagataga tatattgatt
    16741 ttcaggtagc agccacttgc gggcaatttg ctgaattatt tccgggttaa cttttctaat
    16801 ccgttcttct ctggacagat aatattcggg ttcatatccc aaagctgaca ggttagacat
    16861 catagatgcg gtataggaaa cactttcatc ttcaaaacgg ttcattccga ttaaatattg
    16921 tttggcattt tccaattcat cttcagcaac ttctttttct gtaagagaat aaagaatatc
    16981 ctgcattaaa gtaagacaat tacgataatc atcgcggtca caaaaggcat aagcactcca
    17041 aaaaccaagt tcatttaagg agtgaaattc aaaacctgtt tgatatgcat agccgtattt
    17101 ttcccttaag atataataaa accgcgagga aatatcccct cccaggattt gagatagaac
    17161 atagaaagca gtattttcat cacgactaac agcaggacag gcaaatccgc cgttaaaaat
    17221 aattgcctgg tcggtttttc tgtatttcct tttaaaatgg atagttgaag gtgaatacaa
    17281 gggttttgga tacaatgatt gagatgcttt tttagctaaa ccgaaggtct gttcacaaag
    17341 ttctaatacc tcttccggct tatggttacc tacaattccc aaataaaaat cctttccgat
    17401 attccatttt tcataccatt cctgtatgtc cttaagatga agggagcgaa tgtgagaagg
    17461 atttccggaa ctgcgaaaaa ggttgctatt gtttcccacc aacattttat accagtttaa
    17521 ataggcatag ctaaccggaa tatcattatc cctccgaatt tcatctaaag cggcagaagt
    17581 taaaagagaa agatagttac ggtcaaaatt cggaagatag attatttccg ccagcataga
    17641 caatgccttt ttcagattaa cgcttaaaca ttttccccta aaagtagtag tatccaaatg
    17701 atgaattaaa cggatattaa atccattttc ccgggaaaat tgttgtaact cctgatgact
    17761 atgtttttga gttttataca acaataggga agagcaaaaa tagttgtgcc ctatagtgga
    17821 agttgtttca caaatctggc tgataggagt ggaaagagaa aaaccgctga tgcttttgtt
    17881 tttcagttgt ttaaagagaa cttgcatccc gttggaaaga attatctgat agaagccctc
    17941 agctatggga gtgatttttt ccgtatttat tttcacatct tgatttaagg gcttaatttc
    18001 aagattgaga gaaggtttta gggataaaat tgccgggctg gaatttgaat tttgctgaag
    18061 tgaaacactt ttaagggcat aagctttttc ggcaaaatca acattttcaa cagctccttg
    18121 ataataaaca gcaagccctt cgggaagcca atatttatac atagcttgct gtatatcgtt
    18181 taaagtagtg gcattaattt ctgcaccata gctctgaaga cggttcaggt ctcccacgaa
    18241 tttttccgta gctaccaaac tggcaaggtt ttccatccct tcaaaactga ataaccaact
    18301 attaataata tcctttttaa tcagttccat ttcggattca ggtattccgt agttgagcaa
    18361 ggcagaatat tcagtcctga acaactttat aacttcagag atatatttat cggaaagagg
    18421 agtgatagaa atgactgagg caccggaaag aattccgcaa agggaactta ctttcacgct
    18481 ggaacaaatt ttttcttctt ccaccaggcg tttaaataga cgggatgatt ttcctattgc
    18541 caaataacgt atagctatta aaagagcatt agcgtaagga tgtttttcgc ttaattccgg
    18601 taaagcaatg gcaatagtat tttcatttaa ctctttttgc cgaaagaaat agcgaaatcc
    18661 atttatttcc ggttcaatat ttgttagctg ttttacaggt ttgccccggc ttttccaggg
    18721 agtaaaataa taatccaaat aataatccaa ttccttttgg caaaagtcac cacagataat
    18781 cagaaaagca ttttccggga tatagcggtt tttatagaac cgatgaaggg cttcccaatc
    18841 cgcctgcatt atgctttcag gactacccaa aataggatat ttaagaggac ttttttgata
    18901 ataactgctt tggatatact ccaaaaaatc agtttcgggg tcgtttttat actgttttat
    18961 ttcctctaca atgatttcct tttccgtttt tacatcttcc cggctgaaag tgctctgaaa
    19021 agcaagttga gaaaggatgt gtaaaccctc tttcaatttt tcggcaggaa gatttacata
    19081 gtaacaagtg caatcgtaat cggtgaaagc atttagcatt ccacccagac ctgaggcaaa
    19141 aagggaaatt ccgttaaaag gaaagtcctt ggtagattta aagctcaaat gctcaataaa
    19201 atgcgaataa ccccgctggt ttttattttc ctgaaccgaa cctgttctaa tgtataattg
    19261 caaacaaagg acagaattgg aattatcctg cagggaaata acttccagtc cattagctaa
    19321 aattctgcta acgggtttgg gaaaattgtt taaccctgaa tttatcctgt tattcataat
    19381 gttataagtt tctgcttatc agggcataag cattgtgatt atggacggat tcaaaatttt
    19441 cggcttctac ataaaatccg gttattcgct tatctttatt taattccaga gtaatttccc
    19501 gcacaacatc ttccacaaat ttgggattat tgtatgcttt ttcggttaca tatttctcat
    19561 ccgagcgctt taaaatcgga taaatctcac agctggaatg tttttcggca agctgaatca
    19621 gttcttccag ccacacaaat tcccggtaac ggacttttat aaatatatcc gaacgctgat
    19681 tatgagctcc ataaacgctg attgctttag aacaaggaca aagtgtagtt accggaactt
    19741 ttactccaat ccagagttca aaactatcct taaaagaggc attgaaactg caatcataac
    19801 ccaaaagaga agaaacgcgg gaaaccggtg cctgtttttt tataaaaaag ggaaacataa
    19861 tgtctatata ggcagaatca gctttcaggg ttgttttcaa ctcacttaaa aaagcatcca
    19921 gcttatctat aaagacctca gtatgataac gattcaagac ctccagaaaa cggctcatat
    19981 gtgttccccg acgctgatga ggaagttcta catagatatt taaatcggca acgctatgct
    20041 gaatgccttt ttttctatcc tgcactacaa taggataacg aatacctttt acaccaactt
    20101 tatctatggt tattttgcgg ttatcagctt gggattggat atcaggtata ctcataactt
    20161 ttcgttcaag tcccttttct tcatcccttt cgttttcagg gaaacgagag aatcatcttt
    20221 agtataataa atgattgctt cacaatcggg aatcggttta attattgaaa ttgcagtttc
    20281 cggaggcaac aaaaaaagtg cagttgaaag accgtcagac caagcggcag aaggacaaag
    20341 aacagttact gaatgcacat cgggaacggg ataacctgta aaaggattga tgatatgatg
    20401 ataacgaaca ccctgatatt cataaaactg ttgataatca ccggaagtag aaacactttg
    20461 attcagcact ttgaaactgg caatgtaatc atctaagcgg cgaggatgct gaatataaac
    20521 aatttggggt ttttgaaaac ccataaaagc catagaactg cgacaattta taaatccgct
    20581 atccaaattt aaggtctgca tatactctct tgctttatcc aaaatgtaac ctttggcaat
    20641 agcgccaaaa gtgagttgca tacctaccgg cttaaacagt tgttggggag tatattttat
    20701 cttactaaaa tccactttct ctaacatttt ctttatttca agcgaatctg gaggcacagg
    20761 tttttcagca ttaaaacccc ataaatccca taccggcttg atagttgggt caaaagcgcc
    20821 ttcagtcatt ttatataaac tatccgctat cgtgagcaaa ttatatacat cggggtccat
    20881 ttcaaagacc gtatttaaag tatcagcatt gattttagcc agatagcttt ggggattgaa
    20941 ttcgtttaat ttatcttcca gattgcggat ataattgaaa acagcatcta tttgtttgcc
    21001 aacattttta ctttgcgaag tggcagatat ttctactatg gtatccatta agtattgact
    21061 tttaagttca gtaaaggaac gagtcaaata tttgtaggtt ccgaaaccaa tcactaaaat
    21121 caaaatgatg agggaaataa tttctctgcg attcataaaa tataaaacct cataaacgat
    21181 aattcagcag ttcaaagtca attaagcgtc tggtatagtc cctcagggca tatagggaaa
    21241 gaggcaaaat caatgcttcg ggaattaaaa gcagccaaaa aaacagacaa cccaaagcat
    21301 ttaagatagc taaaactaaa gccagaagat agatattcca gcgaacatcc tgaaaatgct
    21361 gataggattt tttgattgcc ttccagggat tataagacat ctcttccatc aaaaccaaag
    21421 aaggcaacgc aatggctacc caataattaa ctaaaaccag ccaaaccaaa cgcaaaatcg
    21481 gagcagacgc aaaattgggc aaaccaaaac aaattatata gataaggcaa aaatataagg
    21541 cattgataag agtccaaaga aaataacgcg gataactacg cagtgcggaa aacatttccg
    21601 agagcttaag atgataatca ttatgtttac aaataggatt aaaagcaatt aaaataaaag
    21661 gaataaaaag agaaaaggca agggacttga taagcaggtt taataacatc tgcgtagccg
    21721 tattacattc cagttgaatt ataccgctaa aaagccaaat taaaaccgca attggaatca
    21781 aacctaaaat aatggcaaaa ccagctccag gtagggaaga ggacattttg ctcttgtatg
    21841 ctttccaggt ttctgagaag gacttcattg aatagagttc atttttgatg acaggtgtct
    21901 gtttcccgca ttgcatacac caaatatcgt gaacagcaag tcgggcatta caatatttac
    21961 aacgcattta tgctcctttt ttgggggtct gctgaatttc gctttagcaa ataaagcaaa
    22021 aaataaaaaa cactactttt gtcaagcacg ggttaacatt tactccaacc gcaggcagga
    22081 cattttaaac atccttctgt gtgttcaata gtatttccgc agtcagggca tagagcaaaa
    22141 ccgtttccaa ccgactgatg agaacctgga ttttcatttt ctggcgataa ttcgccggct
    22201 tggatttgtc ctttagtata aagagttaca aattgttcca gtgcctgacc aactgcatct
    22261 ccacaagaag ttatcatttt tcctttattc cacattgggg attgacaacg gatacctttc
    22321 aattgacgag taatggattc aatcttgatc tgagagcgca aagccaaaga aacaagacgg
    22381 gcaatagctt ccagttgtgc agaagcacat cctcccactt ttcccatttg aataaagacc
    22441 tcacaggcac cttgagcatc ggtattaata gttacataca tatgaccgca acccgtttcc
    22501 aggcgttgag ttatgccttt agtaatttcc ggtctggttc tgggagctac ttttttatcc
    22561 gttttttccg gtgaggttgt ttgagttcct aaatagagaa cctggctttc tcggctgcca
    22621 tcccgataga cagtaactcc tttacagcca agatgatagg caagctcata ggcactttgg
    22681 atatcttcca ccgtagcgga atttgggaaa ttaatagttt tgcttacagc attatcagta
    22741 tatttttgaa aggctgcctg cattctgata tgccattcgg gacttatgtc atgagcggtt
    22801 tgaaacacct ttttcaagtc atccggaata ccctcaatac cggcaagaga gccacgcgtt
    22861 aaaatttctt ctaaaagctt attattatat attccttttt cttccaatgc cttctgcaaa
    22921 tatttattca catagattaa tttctctcca tccattacat ttttcacata agcaagagaa
    22981 aattggggtt caattccgct ggaagtatcg gcaatcatac taattgttcc cgtaggagca
    23041 atagtagtaa gggtagcatt gcggattccc tcttttttta ttttttcttt taaggaatcc
    23101 cagggttggt ttattttttc tcttttcaag taaccggtag cgtagatact ttcctgaaaa
    23161 ttggggaaag cacctttttc ctgggcaagt tctgaagagc gttctttagc ataaaaatct
    23221 ataaattcca taagtttagc ggcaagagca accgcttctt cactggcaca aggtattttt
    23281 aattgaaata ataagtccgc ccagcccata actcccaatc cgattttcct gttagcttgg
    23341 gaaatttttt caatttgagg taggggaaac ttggaaacat caattaccgc atccaggaaa
    23401 tcaacacttt cacgcaccac ttctttcagt ttatcccaat ccacatcacc gtctttaatc
    23461 agcaaagcaa gatttataga acccaaattg catgcttcat aaggcagcaa gggctgttct
    23521 ccacaaggat tagtagattc tattttgccc agtgcaggag tgggattggc attatttagc
    23581 cggtctaaaa acacgattcc cggttcgcca tttttatgtg ccatttttac gatcagggca
    23641 aatacatctc tggctttcag ctttttataa atcactccag tatgaggaga aataagatta
    23701 tattcatcat cattggcaac cgcctgcata aaatcttctg ttatacctac actgatattg
    23761 aaattggtta gttctgcggg attttctttg caggttataa aatccagaat ttgaggatga
    23821 tctacattta agattgccat attagcgcct cttctggttc caccttgttt aacagcatcg
    23881 gtagcagcat taaaaacctt taaaaaggat agaggtccgc tggaaacacc attagtagaa
    23941 cgaactcggg cattggcttc gcgtaagtga ctgaaagaaa aaccggttcc acctccgctc
    24001 ttatgaatta aagccgcatt tttaaccgtt tcaaaaatgc tttccatact atcttcaata
    24061 ggcaaaacaa aacaggcgga gagctgttgt aaatcaccac cagcattcat taaagtagga
    24121 gaattaggta aaaaataacc cgcatccatt aactgataaa aacgattggt tttttcctca
    24181 tcaccgcttg caatattgga ggcaacgcga gtgagcattt tatcccagtc ctctatcaaa
    24241 acgccatttt catttttacg aaaatatctt ttttccaaaa ccttcagcgc attttccgtt
    24301 agtttcatag ccaccccttt tatttattta atgccaaaat ctctcttgct tgttccggag
    24361 tagccagagg gcgaccaatt tcttttgcaa tgcgtgctaa acgagctacc aattgcgcat
    24421 tagattcggc aattactccc ttatgataaa aaatgttatc ttcaaatcca cagcggatat
    24481 gaccacctgt aaccattgct attaaagagg taggaatatg ccatctgcca attcctgcca
    24541 cagcccaggt tgccgtaggg atttcttctt tcaaatgttc catcatatac atcaggtttt
    24601 tgggcttgcc actcattcct ccgggaacac caagaacaaa ttgaatatgc aaaggatttt
    24661 gagttataat tcctttttta attaaacgag caactgcatc aaccataccc gattcataaa
    24721 cttcaacttc aggaactaca ttatattgtt tgaatgcttc tgccaaacga ataatatcgg
    24781 caggatgatt tatgaaaata tcgtcaccaa aatttaaagt tccggcattc agggttgcca
    24841 tttcgggttt taaagccaaa ggagcaagtc gtttatcaaa tgattcgcca actgcacctc
    24901 cagtactgat ttgaataata atctcaggaa ctgctttgcg aatagcagaa atggcttcct
    24961 gaaagcgatc caaacgctga gaaggtctgc cgtcatcttc cctaatgtgt aaatgaataa
    25021 ctcttgctcc ggcttcaaaa caagcttttg cttcttttgc ctgttcttca ggagttatag
    25081 gcaaattggg ttgatcggca cgggttgttt cagctcccgt aatggcagca gttaaaatta
    25141 agggttccat aatgttacct ctttatattt aatctattta tctaaaatat aatatctaat
    25201 gctttgaaat tatgctcgtt cccaatagca ttactttagc aatactcacc cattgctaat
    25261 ggcattagta atgggtaagc aatgagtaag caatgctatt aaggaagaaa ctattcttat
    25321 tcatttttat atttttacgc atcataattt tgatcagtcc ttaaaccagc tttcataaag
    25381 atgcacccgg gttaagttac caatattatg ctgcaaaatg cgttcggcaa tttcggcaaa
    25441 tttattttca ttgtcgctca cataaaaaga atctctgcct ttttgtccat cattttcatc
    25501 actcagataa cggcttaagt attcagccac agccattgcg ctatctacaa gtgtaacttc
    25561 ttctcccata acctgttgaa tggttttaga tagtaaggga taatgggtac aacctaaaac
    25621 aagagtatct atattctgtt ctttcatttc cttaagatat tcctccgcaa taactttcgc
    25681 tacaggatgc tcctgccagc cctcttccac caacggaaca aaaaggggac aggcagatga
    25741 aactacaaat gcctcaggaa gcaattttct gatagcggaa ggataagctc cgcttcggat
    25801 tgttccttcc gttccaatca ctccaatgcg tttacattta gtaactttaa ccgattgttc
    25861 agcacctggt tcaataacac ctataactgg aatatcgctt aaattttgca aagcgggaat
    25921 tgctactgcg gaagatgtgt tgcaagcaac aattaagatt tttattccct gctggagcaa
    25981 aaaacgggca ttctgcaggg aatagtcaat aatggtatta ggagattttg gtccgtaagg
    26041 cactctggca gtatctccaa aataaataat atcctcattg ggaaaagtat tacggatggc
    26101 tttataaacg gttaaaccac caactccaga atcaaaaata ccgattgctc ttttcatttg
    26161 ttgcctcttg tagttgaaga tttgtaggat ttggacgatg gggacttcgt ccttccggtt
    26221 cttttggacg atggcgactt catccttcca gagggttttg gcttttgtga tgcgctttta
    26281 tgggataagg aactctttgt tttggataca ttatgacgca gtgtttcagg tatgggaaac
    26341 aaataaatat catcttccac ataaccaata ataaccgtta aagtatccat taattgataa
    26401 tatttatgag ttctgcggtt tatatagcgc atttctcttt ccaaatattc ccaattacct
    26461 tctcccaaac tgatttcttt aataactccg gtaaccggaa tttcttctaa ttgaacaatt
    26521 aaaccccggg aattgaccga aataaccatt ccctcataaa gttctccttc cttctctctc
    26581 atcaaagcca agctataaac catttctata tcgcgttctg actggtctgc ctgaatttct
    26641 tgttcagagg caacttcggc aaaatgtttc agttgagaag gagtaaattt ttgattagcg
    26701 gtatgacaca accaagtttt acagagataa tgaacaacca aatcgcaaag acgtcggata
    26761 ggactagtaa aatgtgtgta attttccatt gctaaagcga aatgatgaat atgttccgtg
    26821 ctgtatcttg cttttttcat acttctaagc aaaatgcggt caaaaacctt atgatatgcc
    26881 tcattcggaa aagaattaag caaatactgg atggacttat tcaaattctc atacataacc
    26941 cactttacgc cataatagga aaggagttca atcagccgtt ccagcttgtt aaaatcgggg
    27001 tcgttatgaa tacggtaaat tgttgcagga gccgtctgtg aaaggcgtgt agctacatat
    27061 tcattggcaa ccagcataaa attttctatt attttatggc tttctgtttc ctcagccaag
    27121 gttaaacact taagaaatcc gttttcatca tattcatact caatttccgg taaatcaaaa
    27181 aaaatgtagc cagcattcat tcttttttcg gttaaaagac gcgaaagagt tcttgcttcc
    27241 aataaaatgt cctgtaaagg aatcggtatc tccgtttcct tgccagcaaa aaggtcatct
    27301 acttcatcat aattaagccg ggattgacta caaataacgc tttcatatat ttgctgctca
    27361 ataatttgtc ctttacggtc aaaatccgtt tgcactgtta aacagagctt ttcttcttcg
    27421 ggacgtaaac tgcaaataag atttgataag cgttccggta acataggtat aactctcttg
    27481 ggaaaataaa aactattccc gcgtttgctt gcttcctgaa aaatagcccc ctccactgac
    27541 aaataatgtg ctacatcagc gatatgcaca taaagacgcc agcccttagc cgttttttcc
    27601 actgctatgg catcatcaaa atctttagca gtaatcgggt ctatagtaaa ggaataaaga
    27661 ttacgcaaat ccctccgatt cctgtaatcc tccggtttta tttctgcaga aagcagattt
    27721 gcctctgcaa ttacatcttc aggaaattct aaaggtaaat tatactgacg aataacagct
    27781 aaaagctcaa cttctggctc accggatttt cctaatatct ctatcacttt acctacaggc
    27841 actttcccca tttgcggatt tccccaatta gttactttta aaacaacttt atcgccattt
    27901 tctccctgag cgggatcgga aacaaagaac catctgaata ttttggggtt actgcaaatg
    27961 aaagtgctaa aaccattcct tcttataagt tctccaacaa attgctctgt gcttcttttt
    28021 actactttac ggattatagc gctatcctga tgaccttttc tatattttgg ttcaaaggcg
    28081 acaatatcat tatgaaaagc atttagaata tcttccgcat ctacataata atctttttcc
    28141 ggagttctaa taaaagcata agaaaaacct ctggataagg gtgtagcatc aaaaatgcct
    28201 tgtaacaatt tgggattggg agtgggggag ggctgttttg cttttggcat tttttgtaag
    28261 tgatatttct tgcggtcttt gcttaaaata ccttcttcca tcagtccgct aagaatgcca
    28321 ctaagtaatg ctttttcagc tttattaagt tgaaaagtat ttactatttc attgtaggac
    28381 aatccggtat gtggatattt ctggaaataa tctaagattg cctgctttaa ttcattataa
    28441 ttcataatat tccttagtat tttttcccaa ttttttgcat caggatttca attaattttt
    28501 ctctttcttg tttatcggca gaaaatcgta aggataatcc tctaaaaggg tctaaacgcc
    28561 tgggcattgt aaaagtggaa agcattatac ctcttttgtc ttcataaaaa caaccaaaat
    28621 cggtataggg acgactgatt ttaagaaaaa gataacgcac ctgaactttg tcctcataaa
    28681 gttcatattc ggtcattatg aaatagggga gaaggttacc taaaaccaaa agcatcccca
    28741 gaatgtagta aaaaggttgt tgccagccgg ttacagtgat ataatataat aaaaaggaaa
    28801 gcaaaatcaa gaaaaggatt aatataaacg aagtccaggg acgttctacg agaggccagg
    28861 atttccattt aaggattggc tcaggcaaca ttctgaataa tctcccgaca tttttcaatt
    28921 tcttctttaa ttgttaaaat ccaaggaaaa ctttgagttg tagagaattt tgaacccaag
    28981 gtatttgctt cgcgttgcat ttcctgaatg ataaagttta gtgttttgcc aatatctcct
    29041 tcttcttgaa gggtagaagt aaaggtctta agatgagctc tcaaacggct taattcctca
    29101 ccaatatcat atttatctac ataaattgcc aattcctgga tgaggcgttg ttccaaattt
    29161 tcaactttat aagaaccaat gagttcttca atatgcttgt gcattgttgc aaataactct
    29221 tttttaaaag gttcacaaag ctcggaaaca gcatttattg ccatttcaat ctgttgcata
    29281 gagtcacata atacctgttt tatttgttct ccttcttcca gcattgattc tttaattctt
    29341 tgcagagctt gtgctaatgc ttcatttagc acatttgcta ggatttcatc ttctgccagg
    29401 cggtctacac tttcaataac tcccggctct tgtagtaaaa attctatgga aataacctct
    29461 tcgctggcta aaagttgtgc tgcttctata gccaattcat tgtattttaa taacttggtc
    29521 ttatctaaaa ccaattttgg ttctcggtga tcactaaaat ttactcttac ttcaattgct
    29581 ccacgcgata tgctctttat tatctgcctt cttatctccg tttcgtaaaa actcagctcg
    29641 cggggaagga aaatccttaa atccaaaaag cgggcattta tggattttat ctctaattca
    29701 aggtcaatat tgtctctgct gattaaagcg tttccaaaac cggtcatact tttcattata
    29761 actccttttt atattctcgt gtcttgtcat tcttgttttc tttgtcatcc ctgcgcaagc
    29821 acgaatccat tttattattc tgccttaaaa tggattccgc acccagtgcg aaatgacaaa
    29881 agaaaatgta aaatgacaac aacaaatgtg ggggataagt aattataacg catagcgatt
    29941 aaaccccctg tatgcaagtt tacataatat gccttatcac aactattaac tatctgatta
    30001 aaaacaaata agagtgctcg ctgtggcatc ctttacctca atttgtttca aagttccaaa
    30061 gtcatcttct gtgcccggaa gaactgcggt tttaaaatcg cgtgttttcc ctaaaaccat
    30121 ttttctactt tttttgctaa agctctcaat ataaacttct actttgcgtc caatttgttc
    30181 tctgtacttt ttaagcgaga tttttcgttg cagctcaatc atttgctgta aacgagccag
    30241 tcgttcagct tccggaacct gatttgataa tgtttctgca acagttccgg gtcgcggact
    30301 gaatttatag caaaaagcat catcaaattc tattgttttc attagggata gtgtgtcctg
    30361 aaaatcgttt tctgtttctc ctggaaaacc ggtcataata tctgtagtta ttgctatatt
    30421 tggtatgaac ttatgtaact tttccactaa agatatatag tgctgaacag tgtaatttcg
    30481 gttcattgct ttaagtatat tatcactccc gctttgcaca ggtaagtgta tatgttcgca
    30541 tattttagct gaatttgcca gaacttttat taattcctcg gataaatcct tcggatgaga
    30601 agtaataaat ctcaaccggt agatttcatc caattcgtta agtttaacta acaaactggg
    30661 aaaatttacc tctccattta ggtaagaatt gacattttga cctaaaagag taatatcctt
    30721 tttcccctgt ttaccagcag aaattgtttc ctgaatgata tcctgccagg aacgactccg
    30781 ttctcttcct ctaacataag gaacgataca ataggaacaa aaattattac agccacgcat
    30841 aatagttata taggcacaat aatcattatg ataaacaggt tgaattcctt tatataattg
    30901 agtttcgtcc aaatccgtta aaatggtctt ttctgtttct tcatttagta tttcagtaag
    30961 atgttgatac tgatccacac ctattacaaa atccactgtt aacttgtcct tgtttatctc
    31021 ttcgcccaat cgttgtgcca tacagccaac tacaccaatt ttgagattcg gattttcttt
    31081 cttgcgatga ttttcacttt gaattctgcc taaaaccctc tcttcagcat ggccacgaac
    31141 actgcaagta ttaaatagaa gtaaatccgc tttgcttatt tggtgaactt cctgatgacc
    31201 tgctttagtt aaaatactgg caattaattc actatctgcc acattcatct gacagccgta
    31261 ggtctctata taaaacttca tttttgtgcc ttgcttcttt gtttactgga ataccaatag
    31321 gcataaccaa ctgcaattcc agagttgcaa acaataattt cggaggtctc aaatctgtcc
    31381 agtaaagaaa tgttgaaagc gatacttaat tttaatatag gcagatagct ctcttccaat
    31441 agaaagggta aatatctctg ttcgtctcta acttcaaggg aatgagaaag ttcctgcaat
    31501 tgctttttag tgattgtttt tccttccatt ttttctcttt cgttataacc gcagcggaaa
    31561 ataactccag ccagataagt gtaggtaagt cctatcccaa caagacaata ggaaccttta
    31621 taaggtaaat catttagctt ttgccggata aaattatccg ctgtttcact atctccgtta
    31681 aaatcattta acaaggtaag taaaccaaag ggtaaactat tcccctgttg ttttttttct
    31741 ttatccaagt tgataaactc tgtggaaaag cccccaatat caattgccag ggttgcctct
    31801 tccccacttt tatattgtct tgcggaaaaa taagcatagc ggatttcctc ttgggaagaa
    31861 attattctgc attttatgtt atatttatca gtaatccagt cagttaataa attagcattt
    31921 aaagcgaaac gcattacacc tgttgccaaa agaacctttt cggattccag atatgaatcc
    31981 aattggagca atttacttat tatctgctta acacttgtta gccgtttttc acttatcttg
    32041 ttatgcttaa aggcaactcc caaagcagta gtcaattgag tttgatgaac aatttgtctt
    32101 ctttcaaggt caaagagcac ataattcagg ttgttggaac caaggtcaaa cacgctaagt
    32161 aatcttctat tttgtaattg agtatatttt ttcagtacca aaccggcaac ttctgtattg
    32221 cagtttttat atgttttagt tctaatttta cggggtttaa tgtcctcaaa aatagctttc
    32281 cactgtcctg cacttagctg taatgtatct atctgcagac aattagtata gtttttcagt
    32341 cctgcaatca gggctttttc ttcaggataa ttannnnnnn nnnnnnnnnn nnnnnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32461 nnnnnnnnnn nnnttatatt gcaatcggat atctttatac ttatcttttt tgcgggaaag
    32521 caatccgcag gaaatataat ctttaaaggc agacatactt tcatcagtgc tgaaaggtaa
    32581 acgaaaacaa ccatcaaagc gttggtataa agaccagatt aaattcaata ccggttttaa
    32641 agaatttgca acttcaggag tggaaaagtt tgtgtagccg gaggacgctg ctcctccact
    32701 taattctaag ttattatagt tattaactat cccattttcc ttttctgtgt tttcctcttt
    32761 tgccaaaaga gaactataga taaaatgcca ttcaaaattg gtaaccgcaa aaacaggaat
    32821 atgacaatag gcggcaaaat cacttaccag aaaagggata tcggcaatta tgcaatcaat
    32881 cttctcccgt cgtaaaaatt caatctcccg ttccataatt tcgttgcgtt tagagagtaa
    32941 ttccaataat gaattaattg ttctttttat atctaccgta agattttcac tatgcttaac
    33001 tcctacatct attgcgcggt tgtgtaaaac aaaataatgc ggattgaggt cttgaaaaag
    33061 aaaagccggt ttagtagtaa taatatggca aaaaacacca taatgaataa gctcttccgc
    33121 taatgcacat atccgggtag catgaccaaa accgtgattg gaaacataca aggcaaattt
    33181 caacatttat ctctcctatt gcaggattag tttcttttgt ttgccgttat cttgtatttc
    33241 tatcactaat ttattaggta aacaaattat aggcattgac ttggtaaaac cttgttttac
    33301 acaacgctta tcaggacaat cggcaaattt cattctaact ttaccatttt ttatttccac
    33361 ggtattgtgc tcatcaattt tgatgacttt attctgttct aaagagtaaa ccccaaaaag
    33421 ctgattgtcc ttataaatat aaacttgaga atggggttta ttgcttaaat agtatctggc
    33481 ggaaagaaat atggctaata taatcagtaa tattaaaacc acatctgccg gtttcagatc
    33541 gtttaaaata gaatacttct tcatctatct tcgggaaaac cgtgaagaat atacattacc
    33601 cgcataaaca taagttagat attgggacgc aatttcacaa gcatgttcca cttcctctct
    33661 ggtagtaggt ctaacggtca ttttgtaaca aggacggtaa gcagaaatat gaagcgggat
    33721 atttttatct accgaagcaa taaacttagc taagtcattc agttcttctt cactgttatt
    33781 gtagcccgga ataatcaggt tagtaagttc tatatggaca cccatttctt tggcaattat
    33841 gatgttatct aaaataatat ccagttttcc cccacagagc tgacggtaaa actcatctct
    33901 ataggatttt atatctatat taactgcttt tactactttt aaaatattac gccagggctc
    33961 tttatttata taggcattag taactaaaac aatgtcaatt tcaggtgctt tagcagaaaa
    34021 atccagaata tattcatacc acattaaagg ttcagtgtaa gtgaaagcaa cctgctgatc
    34081 tttttgttgt ttcacaattt caatcaagtc ctcaatagcc acatagcggg taggatattc
    34141 ctgctgactg atttcccaat tttgacaaaa cttacaggtc aaattgcagg aattgggacc
    34201 taaagaaaca atcatagttc caggatggaa gtgatagagc ggtttttttt ctataggatc
    34261 aagtgccaga gaagttgttc ttccgtaagc agtagcaatt aaagaaccgt ttatcacttc
    34321 ccggcttctg cagaaacctt ttttaccttc gcttaaaaca cattcccggg ggcaaagttg
    34381 acagcggatt ttattgcctt ctcgcacata atacattgct tctctcatta ttcctccttc
    34441 agccattttt ccaatgcttt taaatatata tccaaagtat gagcattttc agtcaagacc
    34501 ttttttgccg ctttgcccat tttttctcgt aattctttat ttttataaag agcaatcaga
    34561 tcggcaagca gatcgtcttt attgctgata ataattcccc ctgctacttt cagttttttt
    34621 accgattctt tacaggaata gtgatgcgga ccaattacca ccggtttttt gtaataagcc
    34681 ggttccagag gattatgccc tccgaaatca taaaaagaac cccctacaat agcaatgtca
    34741 cagatgcaat atgcctggtc aaggatacct aatgtatcta ttatcagaat atcctctgct
    34801 ctgctatatt cttttctcat agtgaaacaa ctgtaggaat aattatccat cagttctata
    34861 acttcttcca ggcgtctggg atgcctgaga cctatgatta aatgtaagtt gggaataact
    34921 tcttttagag atggatagag ggaaatcaac aatgcctctt caccgggacg agatgaaccc
    34981 caacaaataa taaaatcgtc acttttataa ccccattttt tcctgagctc ttcggcatta
    35041 taatccttta gggttaacga aaatttcagg ttgccactca catatacagg tctattaaat
    35101 attttacgat aacgcttggc atcatcttta ctttgcgcat ggatttcggt aataggactc
    35161 tgtaaatgcc taaacagaaa tttcaataaa cgataacccc ttaaagtgct tttagacatt
    35221 ctggcattta aaaataaaac cggaacttta ttgcgttttg cccaaagcag catattgggc
    35281 caaatttctg tttccactat gcaaatcaga cctggattga ttcgttttag ttgacgattc
    35341 cgtaaatggg gcaaatcaag aacagataga aatcctttta ctttagggct tattttattg
    35401 gcttcagcac aactggtaac agtgctggta gtaatacaaa gtttaacttc cggatgctct
    35461 tgtaaatgtt ttgtaaccaa tgctttaatg gcagttaatt cacccattga ggcagaatga
    35521 aataaaatcc ctttgtgaat atcttctcct tcggctttaa tgcttgccac atagtttttc
    35581 tgctttaaaa aaagatagag caaaggaaag aaaaggaaat atattgtttc caccaggata
    35641 ttgaaaagcg atagtaaaat cattttctat tgtgtatggc aatgcagaat ttgccatccc
    35701 ttttgaaaat ggcgggagcg acgagactcg aactcgcggc cttctgcgtg acaggcagac
    35761 gttctaacca aactgaacta cgcccccagc gcattaaaaa tagtaataaa tttcctttta
    35821 aaaaattggc ttttcctgtc aagataaaag tttagcattt ccgttagcca aaactgtaat
    35881 tgcttatctt ttaaattagt tagccacatt atattttatt cccattcaat agtagcaggt
    35941 ggtttggagg aaatatcata aaccacccta ctgattccct tcacttcatt acaaatgcga
    36001 ttggcaactt tctgtaaaaa ccacattggt aactgagtaa aattagcagt cataccatca
    36061 acgctatcaa cggctcttaa agcacaaacc tgatcataac tgcgttcatc acccattact
    36121 ccaaccgttc taattggtaa aagaacagca aatgcctgcc aggttgtatt ataaagtccg
    36181 tttttttgta attcgcttat aaatatctca tcagcaagtt gcaggaggtg gactttttct
    36241 tcctctactt ctccgataat tctaacagct aaacctggtc ctggaaaggg atgtctttgg
    36301 ataatggatt caggtaaacc aagcaaagtt cctacttctc tgacctcatc cttaaaaagt
    36361 tctcttaagg gttcaattaa ggccaaattc attttttccg gcaagccgcc tacattatga
    36421 tgacttttta tggttgaaga aattcctgct gcggatgtac tttcaattac atcgggatat
    36481 aaagttccct gtgctaaaaa atgtacatct tcataatctt tggctacctg ttcaaaaacc
    36541 tctataaagg tgttgccgat tattttacgc tttgtttccg gatcggtaat tccttttagt
    36601 ttagaaataa atgttctacc ggcatccaca tagcgaatat ttaaacccgg ataaagagaa
    36661 aaggactctt ttaccctttc cgcttctctg taacgcatca atccggtatc cacaaaaata
    36721 ggcagtaact gatttccgat tgccttataa agtaaagcgg cagtaactga agaatcaacc
    36781 cctccactta accccaaaat aactttttgg ttaccgatct tttctttgat tttctcaata
    36841 ctttcattga taaaggcatt cggagtccag tccgcagtta tttttccaat cctaatcaga
    36901 aaattttcta atacttttat tccgtttttt gtgttcgcta cttccggatg aaactgcaag
    36961 ccataaaaag gtaattcctt atgagctatt acagcataag gggattgatc cgtttttcct
    37021 aaaacagaaa agcattcggg taaactggaa acagcatcac tatggctcat ccagacctct
    37081 tccctatctt ccaatcccgc aagtaattca ctatgattat caatatttaa tactgcagag
    37141 ccatattcgc ttctgtttga ggggagaata gttcccccat aatgagttac caataattgc
    37201 attccataac agattcccaa aatgggaatt ctcagctgcc aaattccttc atccggcttt
    37261 ggtgaattta ttttgcttaa actagatgga cttcctgaaa gtataattgc ttgcggagca
    37321 aaggattgta tttgttttaa acttatctga tagggatgaa tctcgcagta gatgtttaat
    37381 gaacgcactt ttcgggctat caattgcgta tattgagaac caaaatctaa aatcaaaact
    37441 ttattatgca ttggtaaacc taatttacaa aaggattatt gaccttttcc aaaccaatgg
    37501 aggtggtagg accatgaccc ggaaaaacca ggatatcatc atctaaaata aagagcttat
    37561 ttttaatgga atcaattata gcagtatggc ttccgcccgg aaaatcagtt ctgcctatgc
    37621 tttgttcaaa taaagtatct ccgctaatta gatattttcc tgccagcaga caaatacttc
    37681 ctttggtatg acccggtgta tggataactt ttaccttttc ggaaccgagg aaaagctcat
    37741 caccatcgtg cagaataact tgaggagctg ataaatttag ctcaaagccc atataggtac
    37801 tgaaattttt ggtattattg gtaagcattt cggcatccgc aagatgaatt gcaaccggac
    37861 aattcaatgc tttggcaaaa tataaatttc ccccaatatg atctgcatgg ccatgggtat
    37921 taataatcat ttttactttc aattccaatt gtttaatccg ctgcagaaat gtttcactct
    37981 ggaaagcagg atcaattaaa aatgcctctg cactaatgct ctcccaaagc agataagtgt
    38041 ttgtttgaaa gctctccagc aaaacagtgc tttctatttt tatcattttt ttatcccttt
    38101 cttacgaata tgaaatcagg tatttattga ttatctctct atccccttta gtagcagttt
    38161 ttatcatctg accgataatc tgtttattgc taatcctcca aacctcaata tttgcagatt
    38221 tgggcacttt aggatgatac aggataaaga ttgccagtgc caaatctaag gtagcaaaat
    38281 taagttttcc ggtaataaat cctaaaggtc ccatataatc ctgtgcctgt agcatcaatc
    38341 cttcctgaaa gatgtttttg agtgtttcat tttctgcttc atctcttcca ataatcaatt
    38401 ttacctcttc cgtcaagcga aaatgacgac cataagcaag taagtttatt gagtgcaaat
    38461 catcctgctg atgctttttc aggtcttgta agcgtaaaca ataattacga tccgtaagta
    38521 aacaaccacc agcaggagaa ggaaaatcaa ttattcctaa ttcttctgct agagctaatt
    38581 gagccaaacg accacgaccc tgaatagata acatatcttt tttatcaatc caaccctctc
    38641 taagaggtaa tgtgtcggga agtaaatttt gcgacaaggg ccttacgatt aagtccttta
    38701 caccgctaag tttacttaca ttattcaaag catttcgctt ttgactttta ggacgctgcc
    38761 ctaaaacctc gccactaata agataatctg catctttatc cggcatcagt tctgccgctt
    38821 ttttaaacat aaaaccgtga caatcaatac aaggatttag tcctttacca aaaccataaa
    38881 ccggattatc caataatttt atatgttctt ccgaaacatc tattacttcc aaatcaatag
    38941 catttttagc agcataatta attgcccgtt ccggaagtaa atacggagct ttaaaaaata
    39001 ccggatgaac agcatatcct ttactttgca tccatttaac tgccagaata ctatccagtc
    39061 caccggaaaa taatgctatt gctttatgtg ttttcatatt gcctatgccc taaaactgct
    39121 cttttttgtc aagcaggaaa tat
//
LOCUS       DAOOZV010000012        34547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1394_length_34547_cov_59.982431, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000012 DAOOZV010000000
VERSION     DAOOZV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 34547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34547
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_34547_cov_59.982431"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..3105)
                     /locus_tag="PLW08_02455"
     CDS             complement(391..3105)
                     /locus_tag="PLW08_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51495.1"
                     /translation="MRRIFFFLLLIVLLASCNLRENLLLPPEISAADYQTGNTIKVYS
                     DYLIKAANDDSYLMLHKESIADELIHIGDEIVFRKVKTFAMRDSLGFQNNAEAKSNTY
                     QFGVIRSGTIIDLIASINMAEIYTEIKPSSDPFYLVSFNYYLQANPINPTYYNKRRAY
                     FPISATGEYALLSIPEEDNPTLQHNGRDNFYAVLLNNTGAQVAVNFPAAYTSMAGNIT
                     IRLKDNLTDYNKLTAYYPNAAISYPVVELQTEKAIGNCLAYLRILNAGKEFFSRQWIH
                     LSENSVYSWSENDPVSGISNWWIDETGLYSFLKGSGEYFLLSPLENQQEISVPLDGSV
                     NSVFLQQVWFDLRSISLPETQMKVNIAPDISSILADYFQNNPYSFNSPVQAFVINFYK
                     DSELIATLPENNWIEFGFFTSQTENNKNRLFSVCRNNLQDIITYKSPGTSYDANHYVQ
                     VNSYIYSGITSSAAYLYGCLQTAPSQLKVPYYKNRQYLQTENAIISWWNADKTDYDYL
                     ILNLKPALSNHPWLKGEPFYIRAASSLADFCFYTHNEKQSSLPSGFYLALPVFNPQEN
                     YLLFSTFPYSRLKNYLQGTANCYVQKNWLNIYPEFPGMIINCNINYTNPLKLRTYSTM
                     NFFWNDLIFYTYGNAPQETNTIFSFYKTNTLADPYRILSNQYNLSYTSAVYQVSSDSE
                     ENFALFQPVLFFPRTAKGQNLLFYEKTEPFYRLYSFYESASYDPWYFYIDNGFNGIAL
                     ANSGSYASFVDNNPHNSVSVSVRNSTQDEIVSLYQVQFVLPNYFINKGVPSGSILNLS
                     KLNYVSGVDNLLAAYQLSVSTPTGEPINPDFYNIIGAQQEPYIYLPITEVATIKTAHL
                     FYRDQLGNVTELNRVDNFSANYANEYVVVGNCFICTVPNPGIFYITKF"
     gene            complement(3105..4307)
                     /locus_tag="PLW08_02460"
     CDS             complement(3105..4307)
                     /locus_tag="PLW08_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51496.1"
                     /translation="MRYFWLILLIIPCLLFSEDIVTLPFNLISIAYNNMLTGNYEIAE
                     KQYQQLTILYPQERSGWEGLLWSQNAQGKFHKSLKTFSNAKTKLSELDGIYNYYAFAL
                     YRQNRLPEARYYYKQALDNMPANPLANQVSCEGLAYTYLALDNYPKAQKYFSKAAFIS
                     GRQMSAIKPSFNSTVYYKVPGTEKNAYGFRQSAKYKCAELKLNYEYFQLDNAFFRDLY
                     KADFTYQFIPLEVGINGSYLSGEDARVYPAWQLGMELCPKLYPGKIVLNPELFVSFSH
                     YPRFDVQQLSLQPQVLWRDFSFSYALHSVFMDNEPSETDSVHFAQQFCLTKSLPYSFE
                     LGFHYGAGNDTWIIDNSGVIIDTFNQNGSYYGISLGKEFFKHLYLYGYYQKWDSEDLF
                     YFSLSGYY"
     gene            complement(4312..4914)
                     /locus_tag="PLW08_02465"
     CDS             complement(4312..4914)
                     /locus_tag="PLW08_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51497.1"
                     /translation="MKRIILPCLLILAAGFLLGQGKASITDSYNAETARNYTRALQIM
                     EDLSANDPNDEFYILRIAWLQYLLGSYNESMKNYQKSNNLYPSLDAQTGILNCQLALG
                     MWNEAHNQAEHILQNFPQNIAVLSKAAYSTYMLQNYRATADYYQRIVNIFPWDMESRG
                     YLVNNLYLSKDIENARQHYQILKKYAPDSAMAKEYSKIFK"
     gene            complement(4933..7083)
                     /locus_tag="PLW08_02470"
     CDS             complement(4933..7083)
                     /locus_tag="PLW08_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPL51498.1"
                     /translation="MFLAIPDSYATILFSDSVLLGLMLLTITLLSPVVGLSGLFSLIV
                     AILAARILGFEHWESSSGITAFNSLLAGMMVGYYYPMQIISSSTINYLCFLLLVSLFT
                     LLLYLFSSYLFQQFFRLPSMSLSFSIVAILLWYYFARKGYLSNYPFDKILLFSPTLKL
                     PEFWRLFFISLGSIFFTPEVSAGILVSIALLIITRIGFGLALLGWTLNYILMRLAGVQ
                     PGSGVFYSGFNAILIMYAVAGIYLLPGKSSILIGLIATGIGFLFTALFNVVYYYYNAF
                     TGLYTPLAVPVFAFPFNIVVLLVIFSLRLRLKVDKPVMNDYGVFNPELALQTYQERYK
                     RFSHLGIPQFAMPLNGLWTITQGNNGIYTHKLDWAYAWDFEMLNSEGRNYDKENNNLN
                     DYFSFSKPVLASAAGCVVKICEGVADNPIGQVNTKENWGNYVCLSHSYGLYSFYAHLK
                     QGSIRVKVGDYIQKGDCIAQLGNSGRSAVPHLHFQVQLGIEPGSPTRLSHLINYKLIK
                     EDKTLEFIGSGIPKEKETLSALVAEPHLQSILALQQQNEQQLKVTRGKKQSTELWKVH
                     LDLWGNFTIKSTKHTDLSFSVYDGIYNALSLKGNHKSALAAFALLISRLPYSEKQEIY
                     LSDEPALSVIISPALRHIVLLFSPLFPLFSACVHSKIKVNSDFIEVESDITYRILGIP
                     YRNRTGNVSIEKYKGLAALELKKGKKTLLKAETI"
     gene            complement(7116..8465)
                     /locus_tag="PLW08_02475"
     CDS             complement(7116..8465)
                     /locus_tag="PLW08_02475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPL51499.1"
                     /translation="MKKHYTAPYIERNSSGNLNKYAHTTVVRHQDNIDGVPIKILVQK
                     YGTPLFVFSEKQIRRTYRRLLETIRIHYPKVDIVWSYKTNYLAAICNIFHQEGAPAEV
                     VSRMEYEKARSNGISGNRIYFNGPSKRKQDLQIAIKEGAYIHIDHLEELYQIENIAEE
                     LAIKPKVAIRVNMDTGISPMWTRFGFNYENGEAYRTVQRLISGNKVQLVGLHTHIGTF
                     ILDPNAYYWAATKLLNFAQTIKDNFGIVLDYIDMGGGFASNNTLIDQYTPGELASPSF
                     EQYAEAIGTAFNASHYVKEYHPRLILETGRVLIDESGYLIASVLGKKNLPTGERAVIL
                     DAGVNINITAWWYKQRVLPTKPFPGTYQNTVFYGPLCMNIDVIRSAMPFPDLYYGDTV
                     IISPVGAYNVTQWMQFIEYRPNVCLINETGEVDVIRSQEDLSDLTSREYIPDHLRQI"
     gene            complement(8479..9546)
                     /locus_tag="PLW08_02480"
     CDS             complement(8479..9546)
                     /locus_tag="PLW08_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPL51500.1"
                     /translation="MLQLNDLQISIAVSGLKTGDNPQPGIPVIRSLRAAGFKGKIIGF
                     VYDAMESGIYLDDIADEIYQMPYPSTGAESFLTRLNYIHSKSKIDVIIPTLDSEIPLY
                     IRLHKELKALGIHTFICTEKQFNLRDKSKLFQYFTSKDVAVPKTVLLNSVAEINKAIQ
                     DIEFPVFIKGRLYEAYRANNIIEAQKYFWELQAKWGLPVIMQELMEGDEFNVVIVGDG
                     KGNCLGMVPQRKLVITDKGKGFGGVVVNNPALEKFARKIIQILSWRGPCELEIIKDKE
                     GAFHLLEINPRFPAWVRLAEGSGQNQPAATVLLALGEIIEELPPFKPGVLFIRHSEDI
                     ISDINLLGEISVNGELIRMHN"
     gene            complement(9551..9799)
                     /locus_tag="PLW08_02485"
     CDS             complement(9551..9799)
                     /locus_tag="PLW08_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPL51501.1"
                     /translation="MNTDQLMTLAVNKNGFVFDPENGISYTVNETGLFVLQQLQSGAT
                     QDEIIKCLTEEYEVTEKNAISDVDHFMAMLKALNLIED"
     gene            complement(10144..11685)
                     /locus_tag="PLW08_02490"
     CDS             complement(10144..11685)
                     /locus_tag="PLW08_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPL51502.1"
                     /translation="MVGIVLSYTTLGIDAYQISVEADIGTGFNPMNIVGMASNAVKES
                     KDRVIAAIKNSGYQISTQHYTINLAPADLKKDSAALDLPIALAILQCNEIFRIIDYDT
                     IAMIGELSLDGFVRPVAGVLPIALAAKKDGINTLIVPLENAEEAAIIEGLNVIPVTSL
                     RECVSWLCKETEIMAANVDREKIFQVLNDFPVDMSDVKGQFQVKRALEVAAAGGHNVL
                     MIGPPGSGKTMLARRVPTILPELNLEEALEATKIHSVAGYSKNFRNGILTTRPFRSPH
                     HTISDVALIGGGAFPKPGEVSLSHRGVLFLDELPEFKRVVLEVLRQPLEDGIVTISRA
                     ASSLTFPAEFMLIASMNPCPCGYFGSNIPNHQCNCEWGSILRYRSRISGPLLDRIDIH
                     VEVPSVSYADLASLPTGDKSVDIRARVNKARAIQHDRFKGTGIFNNSQMNSKQLRKFC
                     ILDDASNALLQNAIDKMGYSARVFDRILKVSRTIADLEGRKDIISDDISEAIQYRTLD
                     RKYWS"
     gene            12102..14309
                     /locus_tag="PLW08_02495"
     CDS             12102..14309
                     /locus_tag="PLW08_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPL51503.1"
                     /translation="MKRTYIFIVILFLALCLNAQPWEMDNSVFNPSGVPSFSFSQPRF
                     MDLDSDGDFDFWLGNTNRPPLYLQNNGTATNPVFAPGPDLLSGIPYLASEIAVSADMN
                     GDGILDLVTGGFSGLHLFLNSGTNANPVFTECGGYFSGLNVGSNPVPGVADVDNDGDL
                     DLVVGLSENGSVIIYFNTGSAAVGNFSESNCQLIGDIGLYAYPVFCDFDSDGKQDILC
                     GRDTHGFVFYQNIGTATNPIWEENNTFFSGLGMSTYWNSPDLVDLNGDGLYDLVYGTA
                     AGPLQYYVHTGTAENPSWQQNTSLFGGVLDVGGASNPVFYDFDGDGDLDLISGNQLGY
                     VKFYRNTGTAYAPAWQEDNSYFANIHHSIYSAVTVGDVDADDLPDVILGDLNGGLYFY
                     HNTGTGLIEQAGVLPPVSVGGWSCPRLIDMDFDGDLDLVVGNEAGNLFYYQNNGTPFS
                     PLWELVNGFFGAIDVGSDCVPTFADVDEDDDLDMVTGNLFGEVQCFLRQRQLWVENTT
                     LFSGIETDQNAAPALVDLDHDGDFDLVLGDYDGTFKFFRNLKYSGSVLNPPQNPIFII
                     DGEVTVTWEEPLTGSTSPFEHYKIYLDGVYCGSTIENIWVFSGLDTGVAYLVGITAQY
                     VAGESLPVTLEFIITGNNDQIVQPITLTNYPNPFNPSTTISFTIPAGSQGTLEIFNVK
                     GQKVRVWNCLPSGEHKIVFNGCNEQGLPLPSGVYFGIIKTQNKVQIRKMALIK"
     gene            complement(14382..16649)
                     /locus_tag="PLW08_02500"
     CDS             complement(14382..16649)
                     /locus_tag="PLW08_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPL51504.1"
                     /translation="MNSEPYHLSGTLHLCGKSHFIADEPPLQGELYAKFLFSPVAHSL
                     ITKLDISAAAKIPGVVKVITSADIPGVNQIGTVIKDEPLFPEKEIMYVGQPLAMVLAE
                     DEYIAELAVKKIILKFEELPAIFAPEDAYSKKQGYIPERKIESGDIENALKNAPYTLK
                     GKTVTTGQEHFYLEGQRCLAVPFEGNRITLQTATQSTMEVQEVASRVLGIPANDVIIE
                     VPRLGGAFGGKERGATIWACMAALGAFLTQKPVLVKLTRDEDMHYTGKRHPFTSLWQV
                     GYDETGKILAYDLLLLANGGAYTDLSIAILERAMFHSDNAYFIPNIRIRGYACRTNLP
                     PNTAFRGFGAPQGIFVIESIMDKIAAQLELDPLEIRKRNAYQNGQLTPYGQEITQSQL
                     NNIFTKLADKANYALLRKEVAEFNKHNCRYKRGIGITPVKFGISFTTTLLNQGSALVW
                     VYIDGSVSVSTGGVEMGQELSTKVAIVVSRVLGIPVNKIRVESSNSQRIGNASPTAAS
                     TGSDINGNAARLAAEKIKDNLAHCAVRLIQQKYNQTIDIKDVIFKDNRVFGKEDPETC
                     VSFEELVRFAHSERVPLAAYGYYKTPGLWFDREKGLGHPFHYYVYGAGLAEVELDTLL
                     GEHKVRKLIIVHENGNSLNEAIDRGQIEGAVLQGIGWCTMEDLKYDAKGVYLSANPST
                     YKIPAIRDLPEKLVIEIIPSLTEEASVFGSKGIGEPPFIYGLSVFFALQSAFKEANPE
                     KELSFPATPESLIQR"
     gene            complement(16646..18121)
                     /locus_tag="PLW08_02505"
     CDS             complement(16646..18121)
                     /locus_tag="PLW08_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPL51505.1"
                     /translation="MENQVTPLETHLNRITFFLNGEKITADIPAGISTLDWLHTQKQI
                     YGTKCSCNEGDCGACTVVIAYPFEGIITYQAITSCLYPAAKLHGKHLITIEGLGTPEK
                     LHPIQQALLTNHAIQCGYCTPGLVMSLFALFAMQPHPDREMTLSALEGNLCRCGTYPS
                     ILNAVDELATHQSVKDIVPAWCREVEKDLFHFAQKEAMLVTKTDIPQQVNCYLIPTTT
                     KQLFDLYAEHPESVFIAGGTDIMVQKNINRKEFPFLIDLAQIPELNRLYLQQDGLHIG
                     SAVTYNQLWESGIVKTDFPVLHNLISQIASRQIRNLGTLGGNIANASPVGDTIPLLMV
                     LNASLWLQSNLELRHLPLKDFFLGYHKTALQKGEIIKEIIIPPLTEDNYVKTIKSAKR
                     KSVDISSVITAVNIEHKNGQITNSALAIGGVAAVPLLSRKFPKLLKSKQIKALDPAVI
                     IKEVESEFEPLTDIRGTTLFRRELIRNHLILYLQELQEEEK"
     gene            complement(18241..18669)
                     /gene="smpB"
                     /locus_tag="PLW08_02510"
     CDS             complement(18241..18669)
                     /gene="smpB"
                     /locus_tag="PLW08_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPL51506.1"
                     /translation="MKTIKNRRAEHDYFIIQTLEAGIALKGTEIKSIRAGKVNFKDSY
                     AKIEDGECWLHNLHISPWEKAAYFNHEAERKRKLLLHKHEIRRLKTKVEEQGMTLIPL
                     ELFINDEGLCKITLALAKGKKAYDKREVMRKKDLERDQEG"
     gene            complement(18666..19985)
                     /locus_tag="PLW08_02515"
     CDS             complement(18666..19985)
                     /locus_tag="PLW08_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPL51507.1"
                     /translation="MIFDIVLEGGSIITMDDKNRILSQSCIAIKDGKIQAIFPIGSSS
                     YEARQKIDTHNCFIIPGLINMHSHLPMTYFRGLADDLPLNTWLQKYIWPLEAKLIKPQ
                     LVYDATLHGASEMIKNGISTTNDMYFYMNSIADACSQVGLRVFISEALIDNESSKEDR
                     DNPIGRRITQLKEEYKDNPLIDFTLAPHSIYACSAETLKTCAKIAKDNNILIHTHLSE
                     IREEVENCLKMHKLKPVEYLKELGLLEVRSIFAHGIWIAEEEMDLLAEKGLSSIAICT
                     ESELKLSSGFAPIKKYQEKGINLCLGTDGVASNNNLDLLVEMSVTAKLHKALNNDPTM
                     LPAQEAFAFVTINAAKALGKEKELGSLETGKIADIAVVSLQELENSPVYNPYSLLVYA
                     INSHSIRDMIIQGKIVMQNRQLCNVNEQQILDKAADYKEMILQATEK"
     gene            complement(19982..21016)
                     /gene="mtnA"
                     /locus_tag="PLW08_02520"
     CDS             complement(19982..21016)
                     /gene="mtnA"
                     /locus_tag="PLW08_02520"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPL51508.1"
                     /translation="MQIDGKQYLSVWFENERVKMIDQNKLPFEFRIAEYSNYLEICDA
                     IREMTVRGAPAIGAAGAYAIALAAQNAPEEKFRAYLRTARDELIATRPTAIDLRNGVN
                     YVYEQTIKFIPDYRHSRQVAILAAREFAKKSIDDCYQIGKIGSEIVPHGARILTHCNA
                     GALATVDWGTALAVIRIAHQQNKDIFVYVDETRPRFQGARLTAFELEQECIPHTIITD
                     SASGFYFWKKEIDLVITGADRICLNGDIANKIGTYEKAVLAKEHKVPFYVAAPFSTFD
                     LATKEGADIPIEIRDEDEIKRINGTYLANPGSPALNPSFDITPAKLITGIITPKGIFK
                     PKDAARQVQI"
     gene            21500..22705
                     /locus_tag="PLW08_02525"
     CDS             21500..22705
                     /locus_tag="PLW08_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51509.1"
                     /translation="MKRNLIFLFIPAIILVAGALFATESRMAGLGFPYGYIKDNSEIS
                     IYPGAIHRYKTQVIGELKSPGSNPDWTLGANLPIKKCVGGIYLNTLTGINVDEMLYDS
                     ESPNLYSRGDLDLSKKIQLYLGFWDKWGIGFGMAIDNIKNDLSDTLDIYKKSSAYYFD
                     LSGGMSTDKCDVGLQVKYAGGSAENELTTYKDFNDEFYRFGVDLAGRYYLLQNQDFEV
                     MCLGKGYFNIGSKKIDIPNPPNKPIEKTTDDNNFMIEVGAGVNYKLNDTNTIIFGFKP
                     VRFASESIEVSKEGTNGSHKMSHSFIYVPEYNLAVESQITRWLTGRVGARQNYVFYSE
                     KDEPSGDGEFEDSEYQSEFVMNLGLGFNFGKFSIDAVLEHDFLHHGPEFIGGKTNGLA
                     SMISLTYQY"
     gene            complement(23233..23418)
                     /locus_tag="PLW08_02530"
     CDS             complement(23233..23418)
                     /locus_tag="PLW08_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51510.1"
                     /translation="MGQKILYSPEQMAVSNGIDTFSIYLSQNLHSRKLRQKKSIFYEI
                     TPVQEKYFYAVLDFCLH"
     gene            complement(24585..25505)
                     /locus_tag="PLW08_02535"
     CDS             complement(24585..25505)
                     /locus_tag="PLW08_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPL51511.1"
                     /translation="MPKVLIATEKPFAADAVQKIKAELEKAGYEYSFLENYTDKSELH
                     NALADAEAVIIRSDIMDEEAFNSAPKLKIVVRAGAGYDNIDLKAATAHNVVAMNTPGQ
                     NSNAVAELAIGMMIYIARNKFNGKSGTELSGKILTLYGFGWVARYVAKIAKGIGMDVY
                     AYDPYLQDYVMLKEGVKPLHSVEDVFKLGDYISLHIPATEETKKCVNWNLLSLVKDDA
                     VLVNTARKEIIDEEALLKVFAEKKKFRYVSDVAPDNSAEILELYPDRVFFTPKKMGAQ
                     TSEANTNAGIAAAKQIIAFFEKGDTTYKVN"
     gene            complement(25543..26670)
                     /locus_tag="PLW08_02540"
     CDS             complement(25543..26670)
                     /locus_tag="PLW08_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPL51512.1"
                     /translation="MQELSQFIQTTGFLNVSWGNLVMIIFGMIFVYLGVAKEFEPLLL
                     IPIGFGIIVGNMPGVAQQGLGVMNEGSVLNYLYFGVSKSIYPSLIFLGIGAMTDFSTL
                     IANPRLILLGAAAQFGIFATFIGSILLGFNVLEAASIGIIGGADGPTSIFLASKLAPH
                     LLGSIALAAYSYMALVPIIQPPIMKLLTTKKERLIRMKPLRVVSKKEKIIFPIVAFVV
                     TTFISPGSVVLLAMLFLGNLLKESGVTERLANSARTVLIDVVTVLIGLTVGAKTTADV
                     FLTPATLKIFLLGAFSFAAATAAGVLFAKIMNLFTKNKINPLIGNAGVSAVPASARVS
                     QVVGQQYDKTNYLLMHAMAPNVAGVVGSAVAAGVLLGILGK"
     gene            complement(26675..27388)
                     /locus_tag="PLW08_02545"
     CDS             complement(26675..27388)
                     /locus_tag="PLW08_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPL51513.1"
                     /translation="MKTYKLLINNEHFEAKIVEYSPTHAKINLNGTEYLVQIEDDKIQ
                     SIPKLAAQEKAVPLAPAFSSSADLYSGEIRAPLPGVIVSILVSQGEEVKKGQAILIIE
                     AMKMQSEIASPFDGKIDKILVKERAPVKEGDLLMTITGAEIKAPPQSKPPQPSVEEPI
                     EKKAVTEKILRAPLPGTIMDIKVKVNDYVHEGDVVLVLEAMKMESDIYSPRTGKISKI
                     YVQKGDLVQDNDPLIEFED"
     gene            complement(27392..28231)
                     /locus_tag="PLW08_02550"
     CDS             complement(27392..28231)
                     /locus_tag="PLW08_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPL51514.1"
                     /translation="MRKIMSLTLFTFCVLSGWAQITSVEQTISDSLALAREKEIISEY
                     GFRDTATLSEVANKLQIEDIDRWKSYLGLEPHNEKLDKMSLRKLGITPYKALLAQQYS
                     IYGFTELSTLEEVAAALKMPVKKIKQEIGLDKPNSPKWDSYSLQSLNLTPEDIKKIHR
                     EFEEKSLNFGLNVTLVGMLTVFTALLITSIIIGQLNHLNRPAKEKKADMKITPGGKVR
                     SASATLNHNVIAALIATIFLHEQSLEEKRKIALTFKRTPTNQWRASAMLNMPNREMYL
                     KRR"
     gene            complement(28242..29792)
                     /locus_tag="PLW08_02555"
     CDS             complement(28242..29792)
                     /locus_tag="PLW08_02555"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPL51515.1"
                     /translation="MENQSPLEKLRAKRAAALLGGGEKRIAEQHAKGKLTARERIALL
                     VDPDSFEEFDMFVTHRCTNFGLDKYIHPGDGVVTGCATINERMVYIFAQDFTVFGGSL
                     SKTYADKICKIMDMALKNGCPVIGLNDSGGARIQEGIDALAGYAEIFWHNVMASGVIP
                     QISAILGPCAGGAVYSPAITDFIVMEKSNSYMFVTGPKVVKTVLNETVANEELGGAKV
                     HTSKSGVAHFIANSEEEAMLTIKLLLSYLPSNNLEDPPYVPSADPITRNCDVLNNLVP
                     ENPNKPYDMLDVIYPIVDDGEFLQVMSNFAQNIIVGFGRMNGRSVGIVANQPKILAGV
                     LDIDASIKGARFVRFCDSFNIPLVVLEDVPGFLPGTMQEHNGIIRNGAKLLYAFAEAT
                     VPKVTVIIRKAYGGAYCVLNSRHMRADIVYAWPSAEIAVMGPDGAVEVIFRKEAMAAE
                     NPKAVLQAREKEYREKFANPFIAAERGYIDDIIEPAETRFRLIRALEMLANKKDSIPP
                     RKHGNIPL"
     gene            30163..30238
                     /locus_tag="PLW08_02560"
     tRNA            30163..30238
                     /locus_tag="PLW08_02560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30196..30198,aa:Lys,seq:ctt)
     gene            30380..31546
                     /gene="metK"
                     /locus_tag="PLW08_02565"
     CDS             30380..31546
                     /gene="metK"
                     /locus_tag="PLW08_02565"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPL51516.1"
                     /translation="MTAALHAINNTDRNGYVFTSESVSEGHPDKICDQISDAILDAYL
                     KADPHSKVACETLATENRIVLSGEVSSRATIDIETIVRKTIKDIGYIHPDKGFDYNCE
                     INNYLHKQEAALEENTGAGDQGLMFGYACSQTKNLMPVPIELAHKLLFNLASARKNGT
                     IKHLLPDSKSQVSMRYLGRKAVDLKCLVISTHHDILSETEFEELKEQIKQLVILPSIA
                     DIEDECKGYFDTKNCEIKINPLGPWQDGGPAFDTGLTGRKIIVDTYGGWAQHGGGAFS
                     GKDATKVDRSAAYMARHIAKSIVASGLAGECLIQFSFVIGEHKPVSLMVDTFGSGKES
                     DAEIERIIARNFDLTVEGIIDYLDLRKPIYFPTASYGHFGREGFFWERVKHISR"
     gene            31578..32372
                     /gene="kdsA"
                     /locus_tag="PLW08_02570"
     CDS             31578..32372
                     /gene="kdsA"
                     /locus_tag="PLW08_02570"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPL51517.1"
                     /translation="MNKLYTELASSQNFFLIAGPCVIEEESIMFTVAETLKELCTRKG
                     IPFVFKSSYSKANRSSVNSFSGPGREEGLKILSKIKQEFEVPVLTDVHECVEVASAAE
                     VCDILQIPAFLSRQTELIKTAAVTGRIINIKKGQFMAPEDMKLAAEKASSTGNKQILI
                     TERGTTFGYHNLVVDFRSFAIMHQFGYPVVYDVTHSLQRPSVGNYSGGNPEFALPMAK
                     AAIATGMVKGLFIETHPEPQKAKSDSASMLPLAKMESLLESCLQVL"
     gene            32394..32954
                     /locus_tag="PLW08_02575"
     CDS             32394..32954
                     /locus_tag="PLW08_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPL51518.1"
                     /translation="MYSKITLVKPNKRVVPWNDLKLMVFDCDGVLTDGRFIYGSTNLE
                     LKHFHGHDGLGFKLLQKTDIMISVISGRSSEALERRCRDLNIPYLFQNITNKLKCLDN
                     LLKDLKLSYTNVVYMGDDWNDVPVMRKVAFSAVPADAQPDVAKIADWVSEYRGGQGAA
                     RDLINYVLMRKGIYEKTVLAYLDSIG"
     gene            32914..33468
                     /gene="lptC"
                     /locus_tag="PLW08_02580"
     CDS             32914..33468
                     /gene="lptC"
                     /locus_tag="PLW08_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPL51519.1"
                     /translation="MKKRFWLIWIVSVSAFILLTSCQQDSLQSATGVLPRDLPDEISY
                     NVKLTQLDNDRSEYYLEAEKIERFYDRRLLYAYKVTLTTYDKNNQISSVIKADTTIVD
                     DARNLIFANGNAILTSPNGTVATQKMVWDRSVDEITAPLMVTITRAGDVMRGQNLRTN
                     SQLSYVEMDAVSAEGIVKEKEIKW"
     gene            33462..34541
                     /locus_tag="PLW08_02585"
     CDS             33462..34541
                     /locus_tag="PLW08_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HPL51520.1"
                     /translation="MVKKGFVFFMLFLLLLPLFGKKKGEEEFRLIHSDKLFMTKMENE
                     QALELNGNVHFFYGEVEFLCRNALILDKQKIARLSGNVIVKNDSLTLEADTLAYYRIP
                     DLMNLGGRITATERTKEGIYRWIKSNFATYDKKNDILTTWSRVHGFDYQENARVKCGY
                     AQWDRGKGYALLLDEPYITSGIEDTLAVAAEKMEYFEPERKLIATFNVQVDRGEFHTT
                     SDFLIYFLEEEKAIFTGEPKFISDFADAKATEFHLFMKERKLVKAELIDSCHIDFAEE
                     RFKEKKNTVDADIVTMDFLDEQLRRFVAEGEVSYFYQQDETEKKDFMINSATGLYLQA
                     NFDEDSKLQNMQMKTDIKGKYVFKK"
BASE COUNT        10643 a         6580 c         6572 g        10752 t
ORIGIN      
        1 taaatagtca aattggaacc ccctgaactt ttacagctcg ggggttcttt tttatgttag
       61 aaattgatag cattttcccc gaatgataat tcccagtccc tatctaattt ggagaattat
      121 ctattatttc ggtgttttcg gtggctaaaa aatatatctg cgaaatctgc ataaggttta
      181 tatactaatg tttcgttgtt aatcttttaa agacacctca catcctctta ggcgatcctc
      241 taacgatcct ctaacgatcc tctaacactt ccttaacgag ataagggaat cgttagagaa
      301 tttttaagcc agtataagaa taagaaatag ccaaattaaa tacactttta actaataact
      361 acagctgact ccccactcag ggaactacat ttagaatttg gtgatataaa aaatccccgg
      421 attgggaaca gtacagataa agcaatttcc tacaacaaca tattcattcg cataattagc
      481 gctaaaatta tccactcggt ttagttctgt tacatttcct aattgatcgc ggtaaaacaa
      541 atgagcagtt ttaatagtag ctacttctgt aatagggaga taaatatagg gttcttgttg
      601 tgctccgata atattgtaaa aatctggatt aattggctct cccgtgggag ttgacacact
      661 aagctgataa gctgccaaga gattatcaac tccagaaaca taattcaatt tgctcaaatt
      721 gagaattgac cctgaaggca cacccttatt aataaaatag tttggtagca caaactgtac
      781 ctgatagagt gagacgattt catcttgagt ggagtttcta acagaaacgc ttacactatt
      841 atgagggtta ttatctacaa aggaggcata ggaaccgcta ttagctaaag cgataccatt
      901 aaatccatta tcaatataaa aataccaggg atcgtaagaa gcgctttcat aaaaagaata
      961 aagtcgatag aagggttctg ttttttcata aaagagcaga ttctgtcctt ttgcagtgcg
     1021 tgggaaaaag agcactggct gaaagagagc aaaattttct tcggaatctg aacttacctg
     1081 atatacagca gaagtgtaac tgaggttata ttgattggaa agaatgcgat agggatctgc
     1141 aagtgtatta gttttataaa agctgaaaat tgtgtttgtt tcttgagggg cattgccata
     1201 agtataaaaa atgagatcat tccagaagaa gttcattgtg gaataagttc tcagttttaa
     1261 cggattggta tagtttatgt tgcaattaat aatcatcccg ggaaattcgg gatagatatt
     1321 cagccagttc ttttggacat aacaatttgc tgttccctgc agataatttt ttaggcgcga
     1381 ataaggaaaa gtggaaaata gaagataatt ttcttgcggg ttgaaaacag gtaaagcaag
     1441 ataaaaacct gaaggcaaag agctttgctt ttcattatgc gtatagaaac aaaaatcggc
     1501 taatgaactc gctgctctga tataaaacgg ttctcctttc agccaaggat gattggataa
     1561 agcaggtttt aaatttagaa taagataatc ataatcagtt ttatcggcat tccaccaact
     1621 aataatggca ttttcggttt gcaggtattg gcgattttta taatagggaa cttttaactg
     1681 cgagggagct gtttgtaaac agccataaag atatgctgca gaagatgtta tcccagaata
     1741 gatataggaa ttaacttgga cataatgatt ggcatcatag cttgttcctg ggcttttata
     1801 agtaataata tcctgaaggt tattacgaca aacactaaat aggcggtttt tattattctc
     1861 tgtttggctg gtgaaaaagc caaattcgat ccagttattt tcaggtaggg tagctataag
     1921 ttcagaatct ttataaaaat tgattacgaa tgcctgcaca ggggaattga aagaataggg
     1981 attattttga aagtaatcgg ccagtatgga agaaatatcg ggagcgatat taactttcat
     2041 ctgggtttcc ggtaaagaaa ttgagcgtaa atcgaaccag acctgttgta aaaatacact
     2101 attaacgctt ccatctaagg ggacggatat ttcctgttga ttttccaaag ggcttaacag
     2161 gaaatattca ccgctgcctt ttaaaaaact gtatagacct gtttcatcta tccaccagtt
     2221 ggaaattccg gacacaggat cgttttcaga ccagctgtat acagaatttt ctgatagatg
     2281 aatccactgt ctgctaaaaa attcctttcc tgcatttaat attcttaagt aagcaaggca
     2341 gttgccgatt gctttttcag tttgtaactc tacaacagga taagaaatag ccgcattggg
     2401 gtagtaggca gttaatttat tgtaatcggt taaattatct ttcaacctaa tagttatatt
     2461 tccagccata gatgtgtatg cagcaggaaa atttaccgct acctgggctc ccgtgttatt
     2521 aagcaaaaca gcatagaaat tatccctacc gttatgttgc agggtaggat tatcctcttc
     2581 aggtatagac aatagagcat attcacctgt agcagatata ggaaaataag ctctgcgctt
     2641 attataatat gtaggattta taggattggc ttgcagataa taattgaaac taactaaata
     2701 aaatggatct gaactcggtt ttatttcagt gtatatttcg gccatattga tagaagcaat
     2761 aaggtctatt atagtaccgg aacgtataac gccaaattga taggtattac tttttgcttc
     2821 ggcattattt tgaaagccca aggaatctcg catagcaaat gttttcactt tacgaaaaac
     2881 aatttcgtca cctatgtgaa ttaattcatc ggcaatggat tctttgtgta gcattaaata
     2941 ggaatcgtca tttgcggctt taattaaata atcggaatag accttgatag tgttccccgt
     3001 ttgataatct gctgcagaaa tttctggggg taacaataaa ttttcccgta agttacagga
     3061 agcaagcaaa acaattaata acaagaaaaa aaagatacga cgcattaata ataacctgaa
     3121 agcgaaaaat agaaaaggtc ttcgctatcc cacttttgat aataaccata taaatagaga
     3181 tgcttgaaaa attccttacc taaggagatt ccataataag aaccattttg attgaaggta
     3241 tctataatca ctccagaatt atctattatc caagtatcgt ttcctgctcc ataatgaaaa
     3301 ccaagctcaa aactatacgg aagagatttt gttaggcaaa actgttgagc aaaatgaacg
     3361 ctatccgttt ctgagggttc attatccata aaaacactat gcaaagcata agaaaaagaa
     3421 aaatcacgcc aaagaacttg aggttgcaaa cttagctgct gaacatcaaa acgtggataa
     3481 tgactgaaag agacaaaaag ctctgggttc aaaacaattt ttcccggata cagttttgga
     3541 cataattcca ttcctaattg ccaggcagga taaactctgg catcttctcc agaaagataa
     3601 gatccattga tgcctacttc caaaggaatg aattggtaag taaaatctgc tttatataga
     3661 tcccggaaaa aggcattatc caattgaaaa tattcataat ttagcttaag ctctgcacat
     3721 ttatatttgg cagattgcct aaaaccatag gcattttttt ctgttcctgg aactttgtaa
     3781 tagacagtgc tattaaaaga cggttttata gcggacattt gcctgccgct aataaaagct
     3841 gccttagaaa aatatttttg tgctttagga taattatcca aagccagata ggtgtaagct
     3901 aaaccttcac aggaaacttg attcgccaaa ggatttgcag gcatattatc taaagcttgt
     3961 ttataataat acctggcttc aggcaaacgg ttttgccgat ataaggcaaa ggcataatag
     4021 ttataaattc catctaattc cgacagtttt gttttggcat ttgaaaaggt tttcagagat
     4081 ttatgaaatt tgccctgagc attttgagac caaagcaatc cttcccaacc gcttctttct
     4141 tgtggatata aaatggttaa ttgttgatat tgtttttcgg ctatttcata gtttccggta
     4201 agcatattat tataggcaat ggaaataagg ttaaatggca aggtaacaat atcttctgaa
     4261 aaaagcagac aggggataat aagaagaatc agccaaaaat aacgcattta gctatttgaa
     4321 tatcttagaa tattcctttg ccatagcaga atcgggagca tatttcttta agatttgata
     4381 atgctggcga gcattttcta tatccttgga aaggtacaga ttgtttacca gataaccacg
     4441 cgattccata tcccaaggga atatattaac aattctttga taataatctg ctgtagctct
     4501 ataattttga agcatatagg tggaataagc agctttactg agaacagcta tattctgagg
     4561 aaaattctgc agaatatgct ctgcttgatt atgggcttca ttccacatcc ctaaagctaa
     4621 ttgacaattc agaataccag tttgtgcatc caaggaagga tataggttat tggatttctg
     4681 atagtttttc attgattcat tataactccc taagagatac tgcaaccagg caatgcgtaa
     4741 aatgtaaaat tcgtcattgg gatcgtttgc acttaggtct tccataattt gtagtgcacg
     4801 cgtataattt ctggcggtct cagcattata agaatcagta atagatgcct taccctgccc
     4861 cagaagaaac cctgcagcca agataaggag acagggtaga ataattcgtt tcattatata
     4921 actccttatt tattaaattg tttcggcttt tagtaaggtt tttttaccct tctttagttc
     4981 caaagccgct aatcccttat atttttctat acttacattt cctgttctgt ttctatatgg
     5041 aatacccaaa atacggtatg tgatatcgct ttctacttca ataaaatctg aattgacctt
     5101 tattttactg tgtacacaag cagaaaaaag aggaaataag gggctgaaaa gtaatactat
     5161 atgccgtaac gcaggactga taattacaga aagagccggt tcatcagata agtagatttc
     5221 ctgtttttca ctgtatggta gacgggaaat taacaaagca aaagctgcaa gagcactttt
     5281 atgattccct tttaaagaaa gagcattata aattccatca taaacgctga agctcaggtc
     5341 ggtatgttta gtagatttaa tggtgaaatt gccccaaaga tcaagatgta ctttccataa
     5401 ttcggtgctt tgttttttac cgcgggtaac ttttagctgc tgttcgtttt gctgttgtaa
     5461 agctaaaata ctttgtaaat ggggttctgc tactaatgca gaaagagttt ctttttcctt
     5521 gggaatacca ctaccgataa attcgagggt tttatcttcc ttgataagtt tgtagttaat
     5581 taaatgtgat agacgggttg gactgccggg ttcaattccc aattgaactt gaaaatgtaa
     5641 atgcggaact gctgagcgtc ctgaatttcc taattgagca atacaatccc ctttttgaat
     5701 ataatcgcca actttaactc tgatgctacc ctgtttgaga tgtgcataaa aagagtaaag
     5761 tccgtagctg tgagaaaggc aaacataatt accccaattc tccttagtat taacctggcc
     5821 aataggatta tcagctactc cttcgcaaat tttaactaca caacctgccg ctgaagcaag
     5881 aaccggttta ctgaaagaaa aatagtcatt taaattattg ttttctttat cgtagtttct
     5941 cccttcacta ttcagcattt caaaatccca agcataagcc caatccaatt tatgagtata
     6001 gattccattg ttcccttgag tgatagtcca caaaccattt agaggcatag caaattgagg
     6061 gatacctaaa tggctaaacc ttttatagcg ttcctgatag gtctgcaaag ccaattcggg
     6121 attgaaaaca ccataatcat tcattacagg tttatccact ttcagccgta aacgcaaact
     6181 gaaaattact agtaaaacaa ctatattgaa cggaaaagca aaaactggaa ccgccagagg
     6241 ggtatataag ccagtaaaag cattgtagta ataatataca acattgaata aggcagtaaa
     6301 taaaaagcca attccggttg caattaaccc aatcaaaatg gaacttttac ctggcaaaag
     6361 atatattccc gcaacggcat acataattaa aatagcatta aaacctgaat agaaaactcc
     6421 gcttcctggt tgcacacccg ccaaacgcat taatatataa tttagcgtcc atcctaaaag
     6481 tgctaagcca aatccgatgc gcgtaattat tagtaaagct atagaaacta agatgcctgc
     6541 agaaacttcc ggagtaaaaa agatagaacc caaagaaata aagaataaac gccaaaattc
     6601 aggtaacttg agtgttggac tgaaaagcag tattttatca aatggataat tggataaata
     6661 ccctttgcga gcaaaataat accataaaag tatagctact atggaaaaag ataagctcat
     6721 tgaaggtaaa cggaagaatt gttgaaaaag atagctgcta aacaagtata gtaaaagagt
     6781 gaaaagagaa actaacagca gaaaacaaag ataatttata gttgaactgc tgataatttg
     6841 catcggatag taataaccaa ccatcatacc tgctaaaagt gaattgaaag cagttatccc
     6901 actacttgat tcccaatgct caaagcccaa aattcttgct gccaaaattg ccacaattaa
     6961 agaaaataaa ccagataaac ctacaacagg agatagtaaa gtaatcgtaa gcaacatcaa
     7021 tcctaaaagc actgaatccg aaaacaaaat cgtggcatag ctatctggaa tagctaaaaa
     7081 cacagacccc acagaccgga aaaatcgtga ccagttcata tttgccgtaa gtgatctgga
     7141 atatattctc gggaagttag atctgaaaga tcttcttggg aacgaataac atctacttca
     7201 ccggtttcgt ttattaggca gacattggga cgatattcta taaactgcat ccactgagtt
     7261 acattataag caccaacagg tgaaataata actgtatcgc cataataaag atcaggaaaa
     7321 ggcattgcac ttctaattac atcaatattc atacacagag gaccatagaa cacggtgttt
     7381 tgataggttc ctggaaatgg tttggtaggt aaaacgcgtt gtttatacca ccaggcagtg
     7441 atgtttatat tcactcctgc atctaaaatt acagctcgct cacctgtggg aagattcttt
     7501 ttgccaagaa cactggcaat aagataaccc gattcatcaa ttaacactct tccagtttcc
     7561 aaaattaaac gggggtgata ttctttcaca taatgagaag cattaaaagc agttcctatt
     7621 gcctcagcat actgttcaaa agatggtgaa gccaattctc ccggagtgta ttgatcgatt
     7681 agagtattat tcgaagcaaa acctcctccc atatctatat aatccaatac aataccaaaa
     7741 ttatccttaa tagtttgagc gaaatttaga agtttggtag ctgcccaata ataagcatta
     7801 ggatctaaaa taaaagtgcc aatatgggta tgcaaaccaa ccagctgaac tttgtttccg
     7861 gaaataagac gctgcactgt tcgatacgct tccccatttt cataattaaa gccaaaacga
     7921 gtccacattg gactaatccc tgtatccata tttacgcgaa tagcaacttt gggttttatg
     7981 gcaagctctt cggcaatatt ttcaatttga tacagttctt ctaagtgatc aatatgtata
     8041 taggcacctt ccttaatagc tatttgcaaa tcttgcttgc gtttagaagg gccattaaaa
     8101 tatatcctgt ttccagaaat gccattactc ctggcttttt catattccat tctactaact
     8161 acttctgccg gggcaccttc ctgatgaaaa atattacaga tagcagccaa ataattagtt
     8221 ttatagctcc aaacaatatc tactttagga taatgaatac ggattgtttc cagcaaacgc
     8281 cggtaggttc gtctaatctg tttttcggaa aaaacaaaca agggtgttcc atacttctgt
     8341 actaaaattt tgatcggaac tccatctatg ttatcttgat gtcggacaac cgttgtatgg
     8401 gcatatttat taagatttcc agagctatta cgttcaatgt agggagcagt ataatgtttt
     8461 ttcattttat ttcctctttt aattatgcat tcttatcagt tcgccattaa ctgaaatctc
     8521 acccagaagg tttatgtcac taattatatc ttctgaatga cggataaaaa gaactccagg
     8581 tttaaaagga ggcaattcct caattatttc acccaaagca agtaaaactg ttgccgcagg
     8641 ttgattttgg ccactacctt ccgctaaacg aacccaggca ggaaaacgag gattaatttc
     8701 cagtaaatga aaagcaccct ctttatcctt gataatttcc agttcgcagg gtccacgcca
     8761 agaaagaatc tggattattt tacgagcaaa cttttccaga gcaggattgt ttactactac
     8821 ccctccaaaa cctttaccct tatctgttat aaccagttta cgctgtggaa ccatccccag
     8881 gcagtttcct ttgccatccc ctacaataac aacattaaat tcatcacctt ccataagttc
     8941 ttgcataata accggtaagc cccattttgc ctgcagttcc caaaaatact tttgcgcttc
     9001 tataatatta ttggctctat atgcttcgta gagacgaccc ttaataaaaa ccggaaactc
     9061 tatatcttga attgctttgt ttatttctgc aacactattt aataggacgg tcttaggaac
     9121 agcaacatct ttggaagtaa aatattgaaa tagtttgctc ttatcacgca ggttaaattg
     9181 cttttctgtg cagataaatg tatgaatacc aagtgcttta agctccttgt gtaagcggat
     9241 ataaagcgga atctcagaat ccaaagttgg aataatcaca tcaattttac ttttggaatg
     9301 gatataattc aagcgtgtta aaaatgattc tgcaccggta gaaggataag gcatttgata
     9361 aatctcgtct gctatatcat ctaaatatat tccagattcc attgcatcat aaacaaagcc
     9421 gattatttta cctttaaaac cagcggctct aagagaacga attaccggaa ttccgggttg
     9481 gggattatca cctgttttaa gaccagatac agcaatgctg atctgtaaat catttaattg
     9541 cagcatacta ttagtcctct atcaaattca acgctttgag cattgccata aaatgatcta
     9601 catcactaat agcatttttt tccgttacct catactcttc tgttaaacat tttataattt
     9661 cgtcttgggt agctcctgat tgcaattgct gtaagacaaa gaggcctgtt tcgtttacag
     9721 tgtagcttat cccgttttca ggatcaaata caaagccatt tttgtttaca gcaagggtca
     9781 ttagttggtc tgtgttcatt tttccctcag cagtttattt tcaggatatg ataatttgta
     9841 tcccgcttat ttagtatatt atgcaaatat caagccaatt tgatcgggaa aaaaaggata
     9901 actttaggga aatatcgtaa ttagcaagtt ataaagggga gttacaaaag ctcctatctt
     9961 atctatatat tgtgaataat tttttttata gctcataaag ctaatgccaa aattgtttac
    10021 tatcccaatt atggattcac cctacagaaa atcatagttt tttttctctc tgtcctctgc
    10081 cctctgctat aataatctgt tcatccgtta aaatctgtct aatgcgtttt ctatctcatc
    10141 aattcagctc cagtattttc tatccagagt gcgatattga attgcttcgc tgatatcgtc
    10201 agagattata tctttgcgtc cttccaggtc tgcaatagtt ctggaaacct ttagtattct
    10261 atcaaagacc cttgccgaat agcccatttt atctatcgca ttttgcagca gtgcattact
    10321 tgcatcatct aaaatgcaaa acttgcgtaa ctgcttagaa ttcatctggc tgttattaaa
    10381 aattcctgtc cccttaaaac ggtcatgttg aatggctctg gctttattta ctctggctct
    10441 gatatcaact gacttatctc ctgtaggcaa agaagccaaa tccgcatagc tgacagaagg
    10501 aacttccaca tggatatcta ttctatctaa aagaggtccg gaaattctgc tccgataacg
    10561 caaaattgaa ccccattcac agttgcattg atgattggga atattggaac caaaatatcc
    10621 gcaaggacaa ggattcatac tggcaatcaa cataaattcc gctggaaaag ttaaactgga
    10681 tgctgcccgg gaaattgtaa ctatcccatc ttcaagcggt tgacgcaaaa cttccaaaac
    10741 cactcttttg aattccggaa gctcatccaa aaataaaact cccctatgcg aaagagaaac
    10801 ctctcccggt ttaggaaaag cacctcctcc aattaaagca acatcactaa tcgtatgatg
    10861 aggcgagcga aaaggtctgg tggttaaaat accattgcga aaatttttgg aatagccggc
    10921 tacggaatga attttagttg cttccaatgc ttcttccaga ttcaattccg gcaaaattgt
    10981 cggaactctg cgggctaaca ttgtttttcc acttcccgga ggaccaatca tcaaaacatt
    11041 atgacctcct gctgcagcaa cttccaaagc gcgtttcact tgaaattgac ctttcacatc
    11101 actcatatcc actggaaaat cgtttaatac ctgaaaaatc ttctcccggt ctacattagc
    11161 agccataatc tctgtttctt tacacagcca actgacacat tctcttaaag aggtaacagg
    11221 aataacattc agaccttcaa taatggctgc ttcttcggca ttttccagag gaacaataag
    11281 agtatttata ccgtcttttt tggctgccag agcaattggc aaaacacctg ctacaggacg
    11341 aacaaatcca tctaaagaga gctcaccaat catcgcaatt gtatcgtagt caataattct
    11401 aaaaatctcg ttgcattgca aaatggctaa agcaattggc aaatccaaag ctgcgctatc
    11461 ctttttcaaa tccgccggag caagatttat tgtataatgt tgagtactga tttgataacc
    11521 ggaattcttg atggcagcaa taactctgtc cttgctttct ttaacagcat tggaagccat
    11581 ccctacaatg ttcatcggat taaaaccggt tccaatatcc gcttctaccg aaatctgata
    11641 ggcatctatt ccgagagtag tgtacgaaag aacaattcca accatatcct tttccttaat
    11701 attttacaaa aaccacaaaa aaagcggaat gtgacaaagt caaggaaaaa gattgtttca
    11761 cccagaccta ataagtgagg aatcatttgt gcctatataa cttttgaacc tcttgataac
    11821 ttttacggca tataggactc atcccgacgg tgaatattaa cctcacgcag attacgcaga
    11881 taaaaaacaa gacatttatt ttatactaac ctactatatt cattcttcat tctccattaa
    11941 tattttcact tagcttttct gataagagga taaaatgaaa aaaacccaga cctattttcc
    12001 ttatctactt ccatattttg catcaacaat atgcagcaaa tgattgacag taaaataggg
    12061 ataaaaaata atagctatat actctcttca ggaggtaaaa aatgaaaagg acttatatat
    12121 ttatcgttat cctttttctg gctttatgcc tgaatgctca gccctgggaa atggataaca
    12181 gcgttttcaa tcccagtgga gttcccagtt tttctttttc ccaaccccga tttatggatt
    12241 tggatagtga cggtgatttt gatttttggc tgggaaatac aaaccgtcct ccactttacc
    12301 tgcaaaataa tggaactgct acaaatcctg tttttgcacc aggacctgac cttttatccg
    12361 gaattcctta tcttgcttca gagattgctg tttcggctga tatgaatggt gacggcattt
    12421 tagatttggt gaccgggggt ttttccggtt tacatttgtt tttgaatagc ggaactaatg
    12481 ctaaccctgt ttttactgaa tgcggaggtt atttttccgg tttaaatgtg ggttccaatc
    12541 ctgttcctgg tgtagcggat gttgataatg acggtgatct ggatttagtg gttggtttaa
    12601 gtgaaaatgg cagtgtaatt atatatttca acaccggttc tgctgctgtg gggaatttta
    12661 gtgaaagcaa ctgtcaactt attggtgata ttggtcttta tgcttatcct gttttttgtg
    12721 attttgattc tgatggtaaa caggatattt tatgcggtag ggatactcac ggatttgttt
    12781 tttatcaaaa tattggcact gctacaaacc ctatctggga ggaaaataac actttttttt
    12841 ccggtttggg aatgtctact tactggaatt cacctgattt agttgatcta aatggtgatg
    12901 gactatatga tcttgtatat ggaactgcgg caggtccttt gcagtattat gtgcatactg
    12961 gaacagcaga aaacccttcc tggcaacaaa atacatctct ttttggaggt gttttggatg
    13021 ttggaggtgc cagtaatcct gttttttatg attttgacgg tgatggtgac ttggacttaa
    13081 tcagcggtaa tcaacttggc tatgtaaaat tttatcggaa tacaggaact gcttatgctc
    13141 ccgcctggca ggaggataat tcatattttg ccaatatcca tcactctatt tattctgctg
    13201 taacagttgg tgatgtagat gcagatgatt tgcctgatgt gattttgggt gatctgaatg
    13261 ggggacttta tttttaccat aataccggaa cagggttaat tgaacaggca ggtgttttgc
    13321 ctcctgtttc tgttggcgga tggtcttgcc cgcgtttaat agatatggat tttgatggtg
    13381 acctggattt agtggttggt aacgaagcag gtaatttgtt ttattatcag aataacggca
    13441 ccccttttag tcctttatgg gaattggtaa atggcttttt tggagcaata gatgtgggtt
    13501 ccgattgtgt tcccactttt gcagatgtgg atgaagacga tgatttggat atggttactg
    13561 gaaacctttt tggggaagtg caatgttttt tacggcaaag acagctttgg gtggaaaata
    13621 caactttatt cagtggtatt gaaactgacc aaaatgctgc tcctgcttta gtggatttgg
    13681 atcacgacgg tgattttgat ttggttttag gcgattatga tggcactttc aaatttttcc
    13741 ggaacttaaa atactccggt tctgtcttaa atcctcccca aaacccaatt tttattatag
    13801 atggtgaagt tactgttact tgggaagaac ctctaacagg aagtacttct ccctttgagc
    13861 attataagat ttatctggat ggtgtttatt gtggttccac aattgaaaat atctgggtct
    13921 tttccggatt ggacacaggt gttgcttatt tggttggtat cacggcacaa tatgttgcag
    13981 gtgaatcatt acctgtaacc ctggaattta taattacggg aaataatgat caaatagttc
    14041 agccaattac tttaaccaat taccctaatc cctttaatcc ttctacaacg ataagcttca
    14101 caattcctgc cggttctcaa ggaactttgg agatttttaa tgtgaaagga caaaaagttc
    14161 gtgtctggaa ttgccttcct tccggagaac ataaaattgt tttcaatggc tgtaatgaac
    14221 aaggtttgcc actaccttcg ggagtttatt ttggcataat aaaaacgcaa aataaggtgc
    14281 agataaggaa aatggcgctg ataaaataag ctatgaggtc aaaagagaga actcttaact
    14341 aaagtcattc attatttcct gaggggctta caccgccatc gctatctttg tatcagggat
    14401 tcaggagtgg cagggaagct aagttctttt tccggatttg cttctttgaa tgcggactgc
    14461 aaagcaaaaa agacagaaag tccataaata aaaggtggct caccaattcc tttagagcca
    14521 aaaacacttg cttcttcagt gagggaggga attatttcta taaccaattt ttcgggtaaa
    14581 tcgcgaatag cgggaatttt ataggtagaa ggattggcac tgaggtaaac cccttttgca
    14641 tcatatttca ggtcttccat agtgcaccag ccaattcctt gtaaaacagc tccctcaatt
    14701 tgtccgcggt caattgcctc atttaaggaa tttccgtttt cgtgaacaat aatcagtttg
    14761 cgaactttgt gttctcccag caaagtatcc agttccactt ccgctaaacc agctccataa
    14821 acataatagt ggaaaggatg ccccaaaccc ttttctctat caaaccacaa cccgggagtt
    14881 ttataataac cgtaagcagc taaaggaact ctttccgaat gggcaaaacg aaccaattct
    14941 tcaaaactta cgcaggtttc aggatcttct ttgccaaaga ccctgttgtc cttgaaaata
    15001 acatctttaa tgtctattgt ttggttatat ttctgctgaa ttagccgaac tgcacaatgg
    15061 gctaaattgt ctttaatttt ttctgcagca aggcgtgctg cattgccatt gatatcgctt
    15121 cctgtagaag cagctgtagg tgatgcattt ccaattcttt gagaattact gctttccact
    15181 ctaattttgt ttaccggaat tcccaagacc cgagatacga caattgctac ttttgtagaa
    15241 agttcctgac ccatttccac tcctcctgta gaaacggaaa cgctaccatc tatataaacc
    15301 caaaccaaag cagaaccttg attcaacagg gtagtagtga aagaaatgcc gaattttaca
    15361 ggagtaatac caattccgcg tttatagcga caattgtgtt tattgaattc agccacttct
    15421 ttccggagta aagcgtagtt tgctttatct gccaatttgg tgaaaatatt atttaactga
    15481 ctctgcgtta tttcttgtcc gtaaggagtt agttgtccat tctgataagc attccttttg
    15541 cgtatttcca agggatcaag ctctaactga gcggcaattt tatccattat gctttcaatt
    15601 acaaaaatgc cttgaggagc tccaaaacca cgaaaagccg tattaggagg taaattagtt
    15661 ctgcatgcat aacccctaat acgaatatta ggtataaaat atgcattatc gctatgaaac
    15721 atagcccttt ccaaaatggc tatagaaagg tcggtataag caccaccgtt ggctaagagc
    15781 aataaatcgt aagccaatat tttgcctgtt tcatcatagc caacttgcca cagagaagtg
    15841 aaaggatgcc ttttacctgt gtagtgcata tcctcatccc gagtgagctt tactaaaacg
    15901 ggtttttggg ttaaaaaagc acccaaagca gccatacaag cccaaattgt ggctccgcgt
    15961 tcttttccgc caaaggcacc tcctaaacgg ggaacttcta tgattacatc atttgccgga
    16021 attcctaaaa cccgggaagc aacttcctga acttccattg tgctttgagt agctgtctgt
    16081 aaagtgattc tattgccctc aaaaggaaca gccaaacagc gttgaccttc cagataaaaa
    16141 tgttcctgac ctgtagttac cgttttgcct tttaaggtgt aaggggcatt ttttaaggca
    16201 ttttctatat ctccgctttc tattttgcgt tcgggaatat atccttgttt tttactgtag
    16261 gcatcttcag gagcaaaaat ggcaggtaat tcctcaaatt tcagaataat ctttttcact
    16321 gcaagttccg ctatatattc atcttctgcc agcaccattg ccagtggctg acctacatac
    16381 ataatttcct tttccggaaa aaggggttca tccttaataa ctgtgcctat ttgatttacc
    16441 cctggaatat cggcagaagt gataactttc accacacccg gtattttggc agcagcagag
    16501 atgtctaatt tggtgataag ggaatgcgct acaggagaaa agagaaattt ggcataaagt
    16561 tctccctgta aaggtggttc atcagctata aaatgggatt ttccacaaag atgcaaggtt
    16621 ccggaaagat gatagggttc agagttcatt tttcttcctc ctgtagttcc tgcagataca
    16681 aaataagatg attgcggatt agttctctgc gaaataatgt cgttccgcga atatcggtta
    16741 agggttcaaa ttcactttcc acttctttta taataacagc cggatcaaga gctttaattt
    16801 gtttactttt caatagtttt gggaattttc tggaaaggag cggtacggca gcaaccccac
    16861 ctattgccaa tgcggaattt gtaatctgtc cgtttttatg ttcaatattt actgcggtaa
    16921 tcacggaaga aatatccaca ctcttgcgtt tggctgattt aatggttttt acataattgt
    16981 cttcagtaag cggaggaata atgatttcct tgataatttc tcccttttgc agagctgttt
    17041 tgtggtaacc taaaaagaaa tcttttaagg gtaaatgcct caattccaaa ttggattgca
    17101 gccacaaaga ggcatttaaa accattaaaa gaggtatagt atctcccaca ggtgaagcat
    17161 tggcaatatt acctcccaaa gttcccaaat tgcgtatctg ccgggaagca atttgagaaa
    17221 taagattatg taaaacagga aaatcggtct ttacgattcc ggattcccaa agctgattgt
    17281 aggttactgc agaaccaata tgtaaaccat cctgctgcag ataaagacgg ttgagttcag
    17341 ggatttgggc aaggtctatt aaaaagggaa actccttccg gttaatattt ttctgcacca
    17401 taatatccgt tccgcctgca ataaaaacac tttccggatg ttcggcataa aggtcaaaaa
    17461 gctgtttagt tgtagtaggg attaaatagc aattaacctg ttggggaata tctgttttag
    17521 taaccaacat tgcctctttt tgggcaaaat ggaataaatc cttttctact tctctacacc
    17581 aggcagggac aatatctttt acactttggt gggtagcaag ttcatccact gcatttaata
    17641 tacttggata agtgccacaa cggcaaagat ttccttccag agcagataag gtcatttctc
    17701 tatcggggtg gggctgcata gcgaacaaag caaataaact cattaccaaa ccgggagtgc
    17761 aataaccgca ttgaatagcg tgatttgtta acagggcttg ctgaatagga tgcaattttt
    17821 ccggagtgcc taaaccctca atggttatta agtgtttacc gtgtaatttg gctgcaggat
    17881 agaggcagga agttattgcc tgataagtaa ttatgccttc aaaagggtag gcaataacaa
    17941 ctgtgcaggc accacaatca ccttcgttac aactgcactt ggtgccatat atctgttttt
    18001 gggtatgcag ccaatccaga gtagaaatac cggcaggaat atctgcagta atcttttctc
    18061 cgttcaggaa aaaagtaatg cggtttaggt gagtttccaa tggggtaact tgattttcca
    18121 tatactttcc ttttcttagg aagaacgata tcctcgtcgt tcatttttaa ccgggttaat
    18181 atcttttccg ataaattcta atgttacggt atacatattt ttctttagtt gacaaggaca
    18241 tcaaccttcc tggtctcttt caaggtcttt tttgcgcata acttctcttt tatcatacgc
    18301 ctttttccct ttagccaaag caagggttat tttacaaagt ccttcatcat tgatgaataa
    18361 ctccaaagga ataagagtca taccttgttc ttccactttg gtttttaagc gtcttatttc
    18421 gtgtttatgc aacagcagtt tccgtttgcg ttccgcttcg tgattaaagt aggcagcttt
    18481 ttcccaggga ctgatatgca aattatgtag ccagcattct ccatcttcaa ttttggcata
    18541 gctatcctta aaattcactt ttccggcacg aatggatttt atttccgttc cttttagggc
    18601 aatacctgct tccagggttt ggattataaa gtaatcgtgc tccgcacggc gatttttgat
    18661 ggttttcatt tttctgttgc ctgtaaaatc atttccttat agtcagctgc cttatccaga
    18721 atctgctgtt catttacatt acagagttgg cggttttgca ttactatttt tccctgaata
    18781 atcatatcgc gaatgctgtg actgtttatg gcataaacca aaagagaata aggattgtaa
    18841 acgggactat tttccagttc ctgtaaagaa acaacagcaa tatcggcaat tttacctgtt
    18901 tccagtgaac caagttcttt ttctttgcct aatgcctttg ccgcattgat ggtaacaaaa
    18961 gcgaatgcct cttgagcggg aagcattgta ggatcattat tcagtgcttt atgcagtttg
    19021 gcagttacag acatttccac caacaaatcc aaattattgt tactggcaac tccatcggtg
    19081 cctaaacaga gattgatgcc tttttcctga tactttttaa taggggcaaa accggaactg
    19141 agtttcagct cgctttcagt gcaaatagca atactgctaa gccctttttc tgctaaaagg
    19201 tccatttcct cttcagcaat ccaaattcca tgagcaaaaa tactgcgcac ctctaaaaga
    19261 cctaattctt tcaaatattc cacaggtttc aatttatgca ttttaaggca attctccact
    19321 tcttccctaa tttcagaaag atgagtatga atcaggatgt tattatcctt tgctatttta
    19381 gcacaggttt ttagggtctc cgcactacaa gcatatatag aatgaggagc taaagtaaaa
    19441 tcaataagcg gattatcttt atattcctct ttcagctgag taattcttct tccgataggg
    19501 ttatccctat cttcttttga ggattcatta tcaatcaaag cttcactaat aaaaaccctt
    19561 aaaccgactt gggaacaggc atccgctata gaattcatat aaaaatacat atcgttggta
    19621 gtgctgattc cgtttttaat catttcactg gcaccgtgaa gagttgcatc atacactaat
    19681 tgcggtttta tcagtttcgc ttccaggggc caaatatatt tctgcagcca ggtatttaac
    19741 ggcaggtcat ctgccaaacc gcgaaaatag gtcattggca aatgggaatg catattgatt
    19801 aaaccgggaa taataaaaca gttgtgcgta tctattttct gccttgcttc gtaagaggaa
    19861 ctgccaatag ggaaaatggc ctgtatctta ccatctttga tagctataca ggattgtgac
    19921 aaaatcctgt ttttgtcatc catagtaatt atggagccac cttccaaaac gatgtcaaaa
    19981 atcatatctg cacctgcctg gctgcatctt tgggcttaaa aatacctttg ggagtgatta
    20041 ttccagtgat aagtttagca ggagttatat caaaagaagg atttaatgcc ggtgaacctg
    20101 gattggcaag ataggttccg ttgatgcgtt taatttcatc ttcatcccga atttcaatag
    20161 gaatatcggc tccttcttta gtggctaaat caaaagtgct aaaaggtgct gctacataga
    20221 aaggcacttt atgctctttg gctaaaactg ccttttcata agtgcctatt ttattggcta
    20281 tatcaccatt aaggcaaatt ctgtcagcac cggttatcac caaatcaatc tcctttttcc
    20341 agaaataaaa accgctggcg ctatctgtaa ttattgtatg tggaatgcat tcctgttcca
    20401 gttcaaaggc agttaagcgt gcaccttgaa aacggggacg ggtttcatcc acataaacaa
    20461 atatatcctt attttgctga tgggcaatcc gaattacggc taaagcagtt ccccaatcta
    20521 cagttgctaa cgcacctgca ttacagtgag ttagaatacg agctccatga ggaactattt
    20581 cactacctat tttgcctatt tgatagcagt catcaatgct ttttttggca aattcccgtg
    20641 ccgctaaaat agctacctga cgcgaatgtc tataatcagg aataaacttg atggtctgtt
    20701 cataaacata attaaccccg ttacgaagat caatggctgt aggacgcgtg gctataagtt
    20761 catctctggc agttcgtaaa taggcacgga atttttcctc cggggcattt tgagctgcca
    20821 aggcaatggc ataagcacct gcagctccaa ttgcaggagc tccgcgaacg gtcatttctc
    20881 tgatagcatc acaaatttcc agataattac tgtattccgc aatgcggaat tcaaagggta
    20941 atttattctg gtctatcatt ttaacccgtt cgttttcaaa ccataccgaa aggtattgtt
    21001 ttccgtctat ttgcatttat tctccatttt ttaggactgc tgtatatatt agtattactt
    21061 ttcaccttta tcattcctga accagcagga atctaaaact gcataaatgc attttccgta
    21121 gacagcggtt atttcgtcaa catctttctt attttttctg taattcttgg aaattaaaaa
    21181 tatttcttgc cagaaagatg gttatggatt aaaatattat aaagaggttt taagttgttg
    21241 ttattgttca taatgaacag attaggagta tataatgtta tgctttatca gaagttgagt
    21301 cctgaaacaa aactttgggg tgttccaaaa ctcaagaagt gcccgaataa aagaaatatt
    21361 tcttttatta gcgagatgat atataaattg ccaaagttgg agaggggaac agtaataaat
    21421 tttccaaatt ctcttttaat cactgaaaaa atattatggc ttagataagc cagagaaaat
    21481 aaaaaagtga ggtaagaaaa tgaaacgcaa ccttattttt ctcttcattc cggcaattat
    21541 tttagttgct ggagctttat ttgctaccga gagcagaatg gcaggtctcg gttttcctta
    21601 tggttatatt aaggataatt ctgaaatttc gatttatcct ggtgccattc atcgttataa
    21661 aactcaagtt attggagaat tgaagtcccc cggatcaaac cccgattgga ctttgggagc
    21721 aaaccttcct atcaagaaat gtgtgggggg aatttatcta aataccctca caggtattaa
    21781 tgtagatgaa atgctgtatg attccgaatc tccaaattta tattcccgcg gtgatttgga
    21841 cctcagcaaa aagattcaat tataccttgg tttttgggac aaatggggta tcggctttgg
    21901 tatggctata gacaatatca aaaatgatct ttctgatact cttgatattt ataaaaagtc
    21961 cagtgcttat tactttgatt taagcggtgg tatgtctaca gacaaatgtg atgtgggatt
    22021 acaagttaaa tatgcaggtg gttcagcaga gaatgaactg actacttata aggatttcaa
    22081 tgatgaattc taccgctttg gagttgatct tgcaggcaga tattatttac tgcaaaatca
    22141 ggattttgag gtaatgtgtc tgggtaaggg atattttaat ataggtagca agaaaataga
    22201 tattccaaac ccgccaaata aacctataga gaaaacaact gatgataaca attttatgat
    22261 tgaagttgga gcaggagtta attacaaact caacgatact aatactatca tctttggctt
    22321 taaacccgtt cgtttcgctt ccgaaagtat agaagtgtca aaagagggaa ctaacggctc
    22381 gcataaaatg tcgcatagtt ttatctatgt tccggaatac aatctggctg tggaatcaca
    22441 aattacccgt tggctgactg gacgcgtggg agctcgtcag aattatgtat tttattccga
    22501 aaaagatgag ccaagcggag atggggagtt cgaagattcg gaatatcagt ccgaatttgt
    22561 aatgaacctt ggtttgggct tcaactttgg caaattcagc atagatgctg ttctggaaca
    22621 cgattttctg catcacggac ctgaattcat tggtggcaaa accaatggcc ttgcctcaat
    22681 gatttcttta acttatcaat actaaaaaac cctttaacga gctggagttc aataccaaat
    22741 cggtattctg aactccagct tcctattaat ttcaaagaaa gataatattt ttaagcattg
    22801 gatttatgcg caaacaggaa tccattcttt ttgccattcc agcgcaagca agtttctact
    22861 taattaaaat ggattccgta acaagtgtgg aataacaatc gtcaacatag caaaagaata
    22921 cacattttgt cattactgcg ttttttgtcc ttccggagat agaaggaatc catagaaggt
    22981 aaaaaatata tttatttttt tcaatctgaa agttatttaa ttcttcatta gagaaaaact
    23041 tcactatcta tccttagtta aaagtgttaa tcaagcgtta acaattaacg cttgataaag
    23101 gtgtgataaa ctatagattg atagtttaag gaaagagtga aatgcactgc tgaattgttt
    23161 ttagtttata ataaaaacga tgattataaa gtgtataata taatttcttg aggggaaaaa
    23221 ttctttatca tttcagtgca agcagaaatc caaaactgca taaaagtatt tttcctggac
    23281 tggggtaatt tcgtaaaaaa tacttttttt ttgtcgcaat ttacgactat gtaaattctg
    23341 acttaagtaa atgctgaagg tatcaattcc attagaaaca gccatttgct ctggtgaata
    23401 aagaattttt tgccccattt atcccgattt taatatttgg gcttaaccca aaaaaagaga
    23461 aagaacttac aatatacctt tccctttaaa aacaagtacc gtaggaacga caggtattaa
    23521 caacgagttt caacccggtg caaagagcag ataaaaacaa catatctcct tgtatcagaa
    23581 agagttccgt aggaacgaca gataataaca acgggtttta acccggtgaa aagagcagat
    23641 aaaaacaaca tatctccttg tatccgaaag agttccgtag gaacgacagg tattaacgac
    23701 gggttttaac ccggcgaaaa gagcagataa aagcaacata tctccttctt ttagaaagag
    23761 ttccgtagga aagacagata ttcaagattt tcattttaat aagaaaataa attttataat
    23821 aatagtattt aattatggag ttaccgaatc ccgattggcc aaaagggaag aacattatta
    23881 tttaccccac tactgttaac tgcttcgcct ttaatatatt atctttttca gcttgcttca
    23941 tcttcttata accaagtaac aaccaagtaa caactccgta acgagacaac ggaagtgtta
    24001 cggaagtgtt acggagtcgt tacagaattt actatatatc aagcaaataa ctatcaagat
    24061 ttattagctc agggaggaat gggatgaagg ggattttaaa gatatttatt caggggaaca
    24121 gtggaaaaag atagatagtt gtttctttct tttttacgag gggcttacgc acccatcgct
    24181 atcattgtgt cgccctccgg gctttgatga aaactcaacc ttctaaacca tctcaacctt
    24241 ctcaaccttt tttcccgagg ggcttacgcc gccatcgcta tgattgtgtc gccctccggg
    24301 tttttaaaaa atctcaacct tctaaacctt ctcaaccttc tcaacctttt ttcccgaggg
    24361 gctaacgcca ccatcgctat gattgtgtcg ccctctgggc tttgatgaaa actcaacctt
    24421 ctaaaccttc tcaaccttct aaaccttctc aaccctctaa accttctcaa cccaatcaac
    24481 tctatcaacc ttctcaacct ttttagttca gattgttctt cagagagaaa acaacggaaa
    24541 gctgaagata agctttccgt tgtaatcgag gaggagggta gtttttagtt tactttgtaa
    24601 gttgtatcgc ctttctcaaa gaaggcaata atttgtttag cggcagcaat tccggcatta
    24661 gtatttgctt cgctggtttg ggctcccatt tttttcggag tgaagaaaac acggtctgga
    24721 tacagttcta atatttcagc actattatcc ggagctacat cactcacata acggaatttt
    24781 ttcttttcgg caaacacctt aagcagtgct tcttcatcaa taatttcttt ccgtgccgta
    24841 ttaacaagaa cggcatcatc ttttaccaaa gataaaagat tccagttaac gcattttttt
    24901 gtttcttcag tagccggaat atgcagagaa atataatctc ccagtttgaa gacatcttct
    24961 acactatgca agggtttaac gccttctttc aacatcacat aatcctgcag ataaggatcg
    25021 taggcataaa catccattcc gatgccttta gcaatttttg ctacataacg ggcaacccag
    25081 ccaaaaccat acagagtaag tatcttacca gagagttccg ttccggattt gccgttaaat
    25141 ttgttcctgg caatataaat catcattccg attgccaatt cagcaacagc attggaattt
    25201 tgccctggag tattcatagc tacaacatta tgtgcggtag cggcttttaa atcaatattg
    25261 tcataaccgg caccagcacg gactacaatt ttaagtttgg gagcggaatt aaaagcttcc
    25321 tcatccataa tatcactgcg gataattact gcttccgcat cagctaaggc attatgcagt
    25381 tcacttttat cggtataatt ctccaaaaag gagtattcgt aaccggcttt ttcaagctct
    25441 gccttaattt tctgaactgc atctgcggca aagggttttt cggttgcaat caggactttt
    25501 ggcatttttg cctcctatat gttttgttgt gtttattttg tttcattttc ctaaaattcc
    25561 taaaaggaca cctgcagcaa ctgctgaacc aacaactcct gctacattgg gagccattgc
    25621 atgcatcaac aggtagtttg ttttatcata ttgttgacca actacctggg aaacgcgtgc
    25681 tgaagcagga actgcagata caccagcatt acctattaag gggttaatct tgtttttggt
    25741 aaagagattc ataattttag caaaaagcac cccggcagca gttgcagcag caaaagaaaa
    25801 agcgcccaat aaaaatatct ttaaagtagc aggagttaaa aaaacatctg cagtggtctt
    25861 ggcgccaaca gttaaaccga ttaaaacggt tactacatca attaaaacag ttctggcact
    25921 attggcaaga cgttccgtta ctccgctttc ttttaataaa ttgcctaaaa aaagcattgc
    25981 cagaagaaca acactgcctg gagaaatgaa ggtggtaact acaaaagcaa caatggggaa
    26041 aattattttt tcctttttgg aaaccactcg caagggtttc atccgaataa gacgctcttt
    26101 tttagtagtg agtaatttca taatcggcgg ttgaattata gggaccaatg ccatatagga
    26161 atatgctgct aaagctattg aacccaaaag gtgaggtgcc agtttggaag ctaaaaaaat
    26221 ggaagttggt ccatcagctc ctccgattat accgatggaa gctgcctcta aaacattaaa
    26281 tcctaaaagg atactgccta tgaaagtagc aaatattcca aattgggctg ctgctcctaa
    26341 taaaataaga cgcggattgg caattaaagt ggaaaaatcg gtcattgctc ctattcctaa
    26401 aaaaatcagg gaaggataaa tgcttttact aacaccgaaa taaagatagt ttaaaacgga
    26461 accctcattc atcactccta atccctgttg ggcaactcca ggcatatttc ccacgataat
    26521 gccaaaaccg ataggaatca gaagcagggg ctcaaattct ttggctacac ccaaatagac
    26581 aaagatcatt ccgaagataa tcattaccag atttccccag ctaacattca agaaacctgt
    26641 ggtttgaata aattggctta attcctgcat aaagctaatc ctcaaactca attaaagggt
    26701 cattatcctg cacgaggtct cccttttgca catatatttt gctgattttc cctgttctgg
    26761 ggctgtaaat atcggattcc attttcatcg cttccagaac taaaacaaca tctccctcgt
    26821 gcacatagtc attaaccttc acttttatat ccataatggt tccgggaaga ggtgcacgca
    26881 ggattttttc tgtaactgct tttttttcta ttggttcttc tacgcttggt tgcggtggct
    26941 tactttgagg aggagcttta atttcagctc cggtgattgt catcagtaaa tctccttcct
    27001 tcacgggagc tcgttctttc actaatattt tatctatttt accgtcaaag ggagaagcaa
    27061 tttcactttg catcttcatt gcctcaataa tgagaattgc ctgacctttt ttcacttctt
    27121 cgccttggga aacaagaata gaaactatca ctccgggaag aggagcacga atttctccag
    27181 aataaagatc ggcactactg ctgaatgcag gagccaaagg aactgctttt tcctgggcag
    27241 caagcttggg aatactttga attttatcgt cttctatctg aaccagatat tccgttccat
    27301 tcaggtttat tttagcgtgg gtgggactgt attccacaat tttggcttca aaatgttcgt
    27361 tattaatcaa taacttatag gttttcatta tttatctccg cttaagatac atttcccgat
    27421 tgggcatatt taacatagcg ctggcacgcc attgatttgt gggagtgcgt ttaaaggtta
    27481 aggcaatttt tcttttttct tccagggatt gttcatgcaa aaagatagtg gcaataagag
    27541 cagctattac attgtgattt aaagtagcgg aagcagaacg aacttttcct ccgggagtaa
    27601 ttttcatatc cgcctttttt tctttggcag gacggttcag atgattcaat tgtccgatta
    27661 taatactggt aataaggaga gcggtaaata cggttagcat tcctaccagt gttacattaa
    27721 gtccgaaatt caagcttttt tcttcaaatt cccggtgaat ttttttgatg tcttcggggg
    27781 ttaagttcaa ggactgcaaa gagtaagaat cccattttgg agaattgggc ttatccagtc
    27841 cgatttcctg tttgattttt ttcacgggca ttttaagtgc agcggcaact tcttccaaag
    27901 tggaaagttc cgtaaagcca taaatgctgt attgttgtgc cagaagtgct ttataaggtg
    27961 ttatacccag ttttcttaaa ctcattttat ccagtttttc gttatgcggt tccaaaccaa
    28021 gatagctctt ccagcggtct atatcctcaa tttgcagttt gttagctact tcagaaagag
    28081 tagctgtatc ccgaaaacca tattcggaaa taatttcttt ttcccgcgct aaagctaaac
    28141 tatcagatat tgtttgttcc acagaggtta tttgagccca gccgcttaag acgcagaagg
    28201 taaaaagggt tagagacatt attttccgca ttgccaatac cttacaaagg aatattgcca
    28261 tgtttgcgag gaggaatgct gtccttttta tttgccagca tttccaaagc tctaattaaa
    28321 cgaaatcttg tttccgcggg ctcaattata tcatcaatat atcctctttc cgcagcaata
    28381 aagggattgg caaatttttc ccgatattct ttttctcttg cctgaagaac tgccttagga
    28441 ttttctgcag ccattgcttc tttacggaaa ataacttcca ctgctccatc aggacccata
    28501 actgcaattt cggcagaggg ccaggcataa actatatccg cacgcatatg acgactgttt
    28561 aaaacacaat atgcacctcc gtatgcctta cggataataa cagtaacttt tggaactgtg
    28621 gcttccgcaa aagcataaag aagttttgcc ccgttacgaa taattccatt atgctcttgc
    28681 attgtgccgg gtaaaaaacc gggaacatct tccaaaacta ccaaaggtat attgaaggaa
    28741 tcacaaaatc gcacaaaacg agctccttta atactggcat caatatctaa aacaccggct
    28801 aaaattttgg gctgattagc tacaattcct acactacgtc cattcatcct gccaaaaccg
    28861 acaataatat tctgggcaaa attactcatc acctgcaaaa attcgccatc atcaacaatc
    28921 ggataaatca catccagcat atcgtagggc ttattaggat tttcaggaac aagattattc
    28981 aaaacatcac aattacgagt tataggatca gcactcggaa cataaggtgg gtcttccaaa
    29041 ttattggaag gcaaataact tagcagaagt ttgatagtga gcatcgcttc ttcttcgcta
    29101 ttagctatga aatgagctac accgcttttg ctggtatgaa cttttgcgcc acctaattcc
    29161 tcattagcaa ccgtttcatt taatacggtt ttaacaacct taggaccagt aacgaacata
    29221 tagctgttgc ttttttccat aacaataaag tccgttatgg caggtgaata tacagctccg
    29281 cctgcacagg gacctaaaat tgccgaaatt tgaggtatca ctccgcttgc cattacatta
    29341 tgccaaaaaa tttccgcgta tccagcaagg gcatcaatgc cttcctgaat tcttgctcca
    29401 ccggaatcat tcagtccaat aacaggacaa ccgtttttta gtgccatatc cattattttg
    29461 cagattttat cagcgtaggt tttggataaa gaaccaccaa aaaccgtaaa atcctgagca
    29521 aaaatataga ccattctttc attgattgtt gcgcaacctg ttaccacacc atctcccgga
    29581 tgaatatatt tatccaatcc aaaatttgtg caacgatggg ttacgaacat atcaaattct
    29641 tcaaaactat caggatcaac caaaagggca atcctttctc ttgcagtgag tttacctttt
    29701 gcgtgttgtt cagcgatgcg tttttctcca ccgcctaaaa gtgctgcagc gcgttttgct
    29761 ctcagctttt ccagagggct ctggttttcc atttcttttc tttcctgttt tttgcttatt
    29821 ttttcaaaag taatgccata gtcatttttt ctaaagcggt tttttagtaa agggaaatgt
    29881 tctcttgttg ctaccctgta aaaggttaaa attgtctctt ttccgctact gtaactgctt
    29941 atacaatcaa taggatcacc gaattcctat ccggcaaatt aatagttata atttatctat
    30001 agctatgatg tttcttgttg ctaaaaaaga agttaccaag ccattttcct tttgtatgtg
    30061 gattgacatc agggaggttg accttctgga ttccatctta ctatttcccc tttttgcttg
    30121 acattatgcc cctctgaaaa aaatatgacc caaatttaca gtgcatcttt agctcagttg
    30181 gtagagcaac tgactcttaa tcagtgggtc cggggttcga atccccgaag atgtaccatt
    30241 tttagaagag cggtgatttg ccctttgctc cgcagtcaaa atgtgctaaa gtgcggtata
    30301 aaaatttttg ccggctttag cagccggctt tttttaaggg gtagcaaaga aagaaagaaa
    30361 taagaattag gagatataaa tgacagcagc attacatgca attaacaaca cagacagaaa
    30421 tggctatgtt ttcacttcgg aatctgtttc ggaaggtcat ccggataaaa tctgcgatca
    30481 gatatcggat gctattttgg atgcctacct aaaagctgat cctcattcca aagttgcctg
    30541 tgaaacatta gctacggaaa acaggatagt tctttcgggg gaagtttctt cccgggcgac
    30601 tatagatata gaaacaattg tgcgtaaaac aattaaggat attggttata ttcatccgga
    30661 caagggtttt gattataact gcgagataaa taactattta cataaacagg aagctgctct
    30721 ggaagaaaat accggagcag gagatcaagg tttgatgttt ggttatgctt gttctcaaac
    30781 gaaaaatctg atgcctgttc ctatagaact ggcacataag ctgcttttta atcttgcctc
    30841 ggcgagaaaa aacggaacta taaaacactt gttgcccgat tccaaaagtc aggtttctat
    30901 gcgttatttg gggcgtaaag cagtggattt gaaatgtctg gttatttcta ctcaccacga
    30961 tattttgagt gaaaccgaat ttgaggaact gaaggaacaa atcaagcagt tggttatcct
    31021 tccttctata gccgatattg aagatgaatg caagggctat tttgacacca agaattgtga
    31081 aatcaaaatc aatcctttag gaccctggca ggatggaggt cctgcttttg atacaggttt
    31141 aaccggaagg aaaattattg tggatactta tggtggttgg gctcaacatg gaggaggtgc
    31201 tttttccgga aaagatgcta ccaaagttga tcggagcgca gcttatatgg caaggcatat
    31261 tgccaaaagt attgttgcca gtggattagc cggtgaatgt ttaattcaat tcagctttgt
    31321 gattggtgag cataaacccg tttctctaat ggtagatact tttggcagtg gaaaagaaag
    31381 cgatgcggaa atagaaagaa ttattgccag aaactttgac ttgacagtgg aaggcatcat
    31441 agattattta gaccttcgta aacccatata ctttcctact gcttcttatg gtcatttcgg
    31501 cagagaaggt tttttctggg aaagagttaa acatatttcc agataaagga gaaaacagca
    31561 ttgctgttct gaaattaatg aataaactct atacagaact tgcctccagc caaaatttct
    31621 tcctgattgc aggaccctgt gtgattgaag aagaaagtat aatgtttacg gtggcagaga
    31681 ccttgaaaga actctgtacc cgaaaaggta ttccttttgt gtttaaatca tcttacagca
    31741 aagccaatcg cagttctgta aattccttca gcggtccggg aagagaagaa gggcttaaaa
    31801 tattgagcaa aataaaacaa gaatttgagg ttccggtgct taccgatgtg catgaatgtg
    31861 ttgaagttgc ttctgctgca gaggtttgtg acattttaca aattcctgct tttctttcca
    31921 ggcagacaga attaatcaag acagctgctg ttacgggtag aataataaac atcaaaaaag
    31981 ggcagtttat ggctcccgag gatatgaaat tagctgccga aaaagcaagt agcacaggca
    32041 ataaacaaat tctgataaca gaacgcggaa ctacttttgg ctatcataat ctggttgtgg
    32101 attttcgcag ttttgctatt atgcaccaat ttggctatcc tgttgtttat gatgtaactc
    32161 attcattgca aagaccttct gtgggaaatt attccggtgg caatcccgaa tttgctttac
    32221 caatggctaa agcagcaatt gctacaggga tggtaaaagg tctttttata gaaacacatc
    32281 cggaaccgca gaaggcaaaa agcgattctg ccagtatgct tcctttggca aaaatggaat
    32341 cccttttgga gagctgtctg caagttttat aaataactat aagggaaata gaaatgtaca
    32401 gtaaaataac acttgttaaa cccaataaac gggtagttcc ctggaatgat ctcaaactaa
    32461 tggtctttga ttgtgacggg gtgcttactg acggtcgttt tatttatggt tcaaccaatc
    32521 tggaattgaa gcattttcac gggcatgacg gtctgggttt caaactgcta caaaaaacgg
    32581 atattatgat cagtgttatc agcggacgta gttctgaagc attggaaaga cgctgtcgcg
    32641 atttaaatat accttatctg tttcaaaata taaccaataa gctgaaatgc ctggataact
    32701 tgctaaagga cttgaaatta tcttatacca atgttgttta tatgggagac gactggaatg
    32761 atgttccggt tatgcgtaaa gtagcttttt ctgcagttcc ggctgatgct caacccgatg
    32821 ttgccaaaat agctgattgg gtttcagaat atagaggtgg acaaggtgct gcaagagacc
    32881 tgattaacta tgtgcttatg cgaaaaggca tctatgaaaa aacggttctg gcttatctgg
    32941 atagtatcgg ttagcgcttt tattttgcta acttcctgtc aacaagatag cttgcaatcg
    33001 gcaacaggag ttttgccgcg cgatttgcct gatgaaatca gttacaatgt taaactgaca
    33061 caactggata atgaccgtag tgaatattat ctggaagcgg aaaaaataga aagattttac
    33121 gataggcgtt tgctgtatgc ctataaagta acccttacca cttatgacaa aaataaccag
    33181 ataagttctg tgattaaagc agatacaact attgttgatg atgccagaaa tttaattttt
    33241 gccaatggaa atgctattct cacttctcca aatggaactg tggctactca aaaaatggtt
    33301 tgggacaggt ctgtagatga aattactgct cctttaatgg taacgattac tcgtgcagga
    33361 gatgtaatgc gagggcaaaa tttacgaacc aatagtcagt taagttatgt agaaatggat
    33421 gcagtttcgg ctgagggcat tgtgaaagaa aaggaaatta aatggtgaaa aagggttttg
    33481 tattctttat gctgtttctt cttttactgc ctttatttgg caaaaaaaaa ggggaagagg
    33541 aattccgtct gattcattcc gataaactgt ttatgactaa aatggaaaat gaacaggcat
    33601 tggaacttaa cggcaatgtt catttctttt acggagaggt agaatttctt tgtcggaatg
    33661 ctctgatttt agataaacaa aaaattgccc gactttcggg aaatgtaatt gtaaaaaatg
    33721 atagtttaac actggaagct gatactttgg cttattatcg tattccggat ttaatgaatt
    33781 tggggggtag aataactgct accgaaagaa ctaaagaagg catctaccgt tggattaaaa
    33841 gcaattttgc cacttacgac aaaaaaaatg atatccttac tacctggagt agagtgcacg
    33901 gttttgatta tcaggaaaat gcccgggtga aatgtggcta tgcacaatgg gatagaggaa
    33961 aaggatatgc attgctgttg gatgaaccct atattacttc aggaatagaa gatacacttg
    34021 ctgtagcggc tgaaaaaatg gaatattttg aaccggaacg caaacttatt gctactttca
    34081 atgttcaggt ggatagaggt gaattccata ccaccagcga ttttttaatc tattttctgg
    34141 aagaagaaaa ggcaattttt accggtgaac cgaaatttat ttctgatttt gccgatgcta
    34201 aagctaccga atttcatctc tttatgaaag aaaggaaatt agttaaagcc gaattaattg
    34261 attcctgcca tattgatttt gccgaagaac gctttaaaga aaagaaaaat acagtggatg
    34321 cagacatcgt tactatggat tttctggatg aacaactacg caggtttgtt gccgaagggg
    34381 aagtgagcta tttctatcag caggatgaaa ccgaaaaaaa ggattttatg attaatagcg
    34441 ctacaggact ttatctgcag gcgaattttg atgaggatag caaactgcaa aatatgcaga
    34501 tgaaaacgga catcaagggc aaatatgtgt tcaagaaatg aagaatg
//
LOCUS       DAOOZV010000013        32786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1504_length_32786_cov_58.266170, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000013 DAOOZV010000000
VERSION     DAOOZV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 32786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32786
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_32786_cov_58.266170"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..3126
                     /gene="uvrA"
                     /locus_tag="PLW08_02590"
     CDS             199..3126
                     /gene="uvrA"
                     /locus_tag="PLW08_02590"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPL51521.1"
                     /translation="MKTAIIIKGAQEHNLKNIDLTIPRNNLVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAISIEQKSASKNPRSTVGTA
                     TEIYDYLRVLYARIGKQYCYNCGRPVGSQTIDQMISHLMQNKPGTKLQILAPIVQNRK
                     GEHKDELEMLRQEGFVRVMINGQLRNLEEEIVLDKKSKHNIDVVVDRIVIKEEIESRL
                     SDSLELALKLAEGIVKIDYPEEKQETILSAQNACPHCHIGFEELSPQSFSFNSPIGAC
                     PLCGGLGYKLEFDPDKVITDPELSILEGAVAPWGRLEAKQHSWTLNTVQSLARAYDFS
                     LSTPWYKLPEIVKQLILYGSKGKKFKNAWQNENGRGEFMMRFEGIIPQLERRMHETTS
                     EEMRRYYLLYISDKPCPACNGNKLKPASLAVKIAGKNISEITAMSIREVYNFFDKLQL
                     KGNEKIIAEEILKEIKNRLGFLMNVGLHYLTLDRRSPTLSGGEAQRIRLASQIGSQLV
                     GVMYILDEPTIGLHQRDVAKLVAMLLQLRDLGNTLIVVEHDEYTIRTADQIVDFGPRA
                     GIYGGEIVATGTLETILQNPKSLTGAYLSRRLKIPVPEKRIKPDDRMLSIINAQHNNL
                     KNLNVDIPLGLFVCVTGVSGSGKSSLINQTLSPFLHNHFYHTAQKVGKMDEIKGFEHI
                     DKIITIDQQPIGRTPRSNPSTYVKLFDPIRELFAQLPASKMRGYTPGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMADIFVTCEVCKGKRYNQETLSIRYKGKNIADVLNMDVQEAIE
                     FFDAIPAIRNKLKTLQEVGLDYLKLGQPSPTLSGGEAQRIKLSRELSKVSTGNTLYVL
                     DEPTTGLHFDDINKLLKVLKKLVAMGNTIIVIEHNMDVIKSADWIIDLGPEGGDEGGK
                     IICSGTPEEVMACPESATGLHLKQQLEKDKEETQNLLLEMNSEDKARKNNLKKKTTKR
                     QAL"
     gene            3123..5297
                     /locus_tag="PLW08_02595"
     CDS             3123..5297
                     /locus_tag="PLW08_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPL51522.1"
                     /translation="MSYQHFSYSPLTAGKHTVGLAGDFTSWEIIPLEEIGGIYTLSID
                     LPPGVYQYKFIVDGNWIPDKNNPHQVSDNFGGVNSLLIVEEEKEEVTWEDILAQLPNK
                     APEKFYQFFRSDVNNYELRFSWYPKLAETINLLTESWNIEFKRIGQNPLYEVFYCLFK
                     QTGIFSFRIRIQYENKALYFGAEGFSEKEEDISPLKINLKDIPLFAIPDWVSRSIIYQ
                     IFPDRFYNGNKANDPDFSEWYYADCKEPPPDGENLSPEKEYYHLVSDWNDISGLKQSP
                     WQKKGIPDFFSFYGGDIAGVRQKLEYLLDLGINVIYFNPLWQAKSNHKYDSADYHSID
                     PHFATTEEMMDFVKIAHQKGIRIILDVAFNHTGETFWAFRDCVEKGPQSPYWNWYDWK
                     KWPLPKPLPPDFNPKEYYQCWWGIKDMPDLNYDLALPHPDENAVRDIRKARPNAPLVD
                     YLISTVRWWLIDIGIDGFRLDVPDEVPFWFWELFRNEVKKAKPDAWIVGEIWHEARQW
                     VSPLYFDSVMNYAFFKTPVIEFFVLNIISKEEFCQKIETGLGLYPFHSLKAMMNLLGS
                     HDTPRIFEICRGNVQRLKLAVIFQMCFIGAPHIYYGDEIAMEGGKDPDNRRPFNWNWE
                     DNPVACEVRAFYKQIISLRKAHPAFWDGHFAFLPAPENAIAFLREGESEKILVFLNLG
                     LDTVTISIPDEGEILFGNIDTKKQLQTLSAVILSLPVQPENS"
     gene            complement(5591..5872)
                     /locus_tag="PLW08_02600"
     CDS             complement(5591..5872)
                     /locus_tag="PLW08_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL51523.1"
                     /translation="MSRDELVKKIAANAGTSQKVASAALTAVLEGITESLKKGEKVSL
                     VGFGSFSVAHRKARNGINPKTKKPLKIPARNVPVFKAGKNLKEAVKKAK"
     gene            6171..7352
                     /locus_tag="PLW08_02605"
     CDS             6171..7352
                     /locus_tag="PLW08_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51524.1"
                     /translation="MKQKIMTFVVLVLLVLLSACGSNKKVQAPVEPVRNPLILAHSAA
                     ARGTEAFNDEFYNDAISAFTEAISLFNEAALTATPADSVAENIEKMNLNIAKSYIELA
                     NESMELSSFDEALDYYSKALNVYKSITPKLITQEELQQNIIGIYNNMAITAKQAGKYE
                     QALTYYDQILKLTPNDADVLYAKFYVLRDNIKDENRAYAVLIDYAELTQDAKAFINVA
                     DSYAEKGNLIEAGKYYQKALAIEPSVDLYRRLANYYSGLKDYNNANLYLQKVADSNPE
                     PSELAQIYALMAKNYEGMNDKKKMVEYYEKSLSIERNPQLALLLASYYNSQKNYAKVI
                     SNANITLSIDPKNADALMLRGVAYYQMKNYSAAKADLERLQNDPKYGTQAKNLLKNIP
                     K"
     gene            7378..7857
                     /gene="umuD"
                     /locus_tag="PLW08_02610"
     CDS             7378..7857
                     /gene="umuD"
                     /locus_tag="PLW08_02610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPL51525.1"
                     /translation="MNWQPVLRSEKTMKLHSDGSIKAIYSATQGKKQYRPLMSSKIPA
                     GFPSPAQDYIEGKLDLNEYLIVHSASTFFVRVDGYSMQGAGIMPDDILIVDRALEATN
                     NRIVIAIVDGELTVKRLKIKGKKYYLVPENDEFEPILIQEGMDFIIWGVVTYVIHKV"
     gene            7862..11110
                     /locus_tag="PLW08_02615"
     CDS             7862..11110
                     /locus_tag="PLW08_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51526.1"
                     /translation="MAINKPCFFSLLFILGITTLFAQSLVIEHNLELEPNRYKYPLAN
                     SPIIKNSVSVWADSLLLVEGTDYWVNHQKAELILLTQPEVPVLKVEYILVPEFLTVPL
                     QNWKPQEYSDTLLTSIKKRKNPILAEDARLDIQGTKTFAITFSDESTFDLKQSLFVNI
                     SGELAHNVYINAQLSDSQSKLSPEGDSKELSSLDNVFIRIYGPKYELAMGDLTLKYTG
                     TRYMEYYSKFEGLNAWLRGKHYVQTAFSAGGGKNASVKITIIDGKQGPYYLRANDTQS
                     NFIVVAGSEEIFVDGSKWERGIDYSIDYSEGSVMFKHLISSSNNVLARFQYSDEYYPQ
                     SSYLNSSQLQLAEHLTLTHHFIWQQDDKKNPLLYEFSSADLDSLKSAGDNFVWGEGIY
                     LVEAGTGDYKLIQLSQIEYYEYAPGDSLACYNIVFSYVGSGNGDYEEYSPGKYRYLGS
                     KMGSWLPQKLLVPPEKKGNLDLAVAYSKDNLNAGVEVLGSVNDRNTFSVKDDEDNDAG
                     LLYAYLEVLFYRYSFRLEHEQRSEKTYLFGKYRNPDVEYDFAAIETADSLAQQESNIQ
                     ISSKQTNWNVSLLLRYRRIYDLYTLQALRFTSSSSGKGLLPAINLHSTLSAQDYANDD
                     VRDGTLQYHQGDISWAWKILRVRFETLYNLLDTKNYGNSYLKANPGITIGNPSSFLTQ
                     LSYTTDKTKLQENDAWNEISSSNTYALKQMINLGEQRIDLDVTHREVQQPYSTSNPKS
                     NYDLINFRSSNSFLKNSIGFYSNYQLNQTEFYPKIRELQYVGSGLGYYDSTGVSVTDG
                     DYDWVYITSSTGSLSSEINCLLNLYWKPGNVSSRDFIKRLQSDTSINLIEQSFQRNNW
                     KSYFFLPGSVYNEETTIYGRQNIEEVLWLDLIRNKLTANLQISMDRTLDKRYQTPDRT
                     FSFSQVAQFDIKGFNAYNTRLQFSNETNRDTHYLSEIDIISASALVQRNLNPQSNIQA
                     ELSCSSESGGKQDGSENYRLYSFAFSPSLKSVFMQKYRLSASLAITYNRLSGSEYFSF
                     LPQKRAGWIPSANLSGVYRINTFCLITLDYRFSDYPKQSSSHELKLEFKAEI"
     gene            11114..11887
                     /locus_tag="PLW08_02620"
     CDS             11114..11887
                     /locus_tag="PLW08_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPL51527.1"
                     /translation="MQSIILSPLDYIIVLPFIFLAGFIDSIAGGGGLISLPAYWSVGI
                     PPHLALGTNKFSSCCGTLFSTANYFKAKMIDIPVALVSAGMAIIGSWLGASTALRVSS
                     QVLNYLLIILIPAVAIFSLLNRNLGMQSKAEFLAKRLRMLLGGIAGLGIGFYDGFFGP
                     GTGTFLILIYTSLLHYDFVTANGNTKVVNLASNLSALITFAFASNIYYPLAVPGALCG
                     IAGNILGSKLVILRGNKLIRNVFILALLLLFARVIYNLV"
     gene            12099..13184
                     /locus_tag="PLW08_02625"
     CDS             12099..13184
                     /locus_tag="PLW08_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51528.1"
                     /translation="MKKQIIIFCLMLLVLTACSTARQKLSPQGNVNLKTADVYYSQQD
                     VEQAEIYYLKVLEDNPDHAIALRRLGDISLFKAENFSAREVEFYEDAYHYYAKAISVT
                     EQFPNLTDQDRIDLRDMKKRKERAWTRIFLAAGKEKEAGNTQRAMEIYELAHKLEPER
                     PEPMIQLKNIYLVELKDDVKAEQILQQLLQKDPDKLEYLMEMGTFYYNKGNYAEAVKY
                     FEKARPQIPTNIDNLMNISACYYELKDFEKAMSATKTALEIEPNNVDLLDNARSIAAQ
                     LNDIDQAIFYLKKLLDIRPNEDDFSLLATYLYNKQNWQELIKYAEQWYNWDKSNKIAV
                     EYIIWAAQQTGNKQLETKYTAIKKTMP"
     gene            13421..14341
                     /locus_tag="PLW08_02630"
     CDS             13421..14341
                     /locus_tag="PLW08_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPL51529.1"
                     /translation="MSKQNKAYLFAGASILAWSTISTAFKLALNYLTPVGLLFFASLT
                     AFLFLGIVNAVSNPSLFKTKELFKTVGRNLKISFLRGLLNPFIYYLMLFEAYSRLRAQ
                     EAQALNYTWAIVLALFSIWLLKEKFRIIDFVSLLISFFGVWVISTKGQITSLQFDDAL
                     GSFLAVSTSIIWAFYWILNIKDKRPALTKLNYNFFLGFLFIALFALFTHKSLFTPQAI
                     YGYGILGGIYVGIFEMGLTFLLWNKALELTDNTAVISNLIFFTPFLSLLFISSVLKES
                     IHYATFIGLLLIFFSNLLQKGLPAILKRNE"
     gene            complement(14324..15385)
                     /gene="dinB"
                     /locus_tag="PLW08_02635"
     CDS             complement(14324..15385)
                     /gene="dinB"
                     /locus_tag="PLW08_02635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPL51530.1"
                     /translation="MKKIIHIDMDAFFAAIEIRENPRLKGKCVIVGGPPNSRGVVSTC
                     SYEARKFGVHSGMSSYQAWNLCPEAIFIHPHFNLYKKVSHQIKEIFYQWTDLVEPLSL
                     DEAYLDVTENKLGEDDAVKIAKLIKAEILEKTGLTCSAGVSYNKFLAKIGSDLNKPDG
                     LTYIPPEKASEVLFALPIDKFYGIGKVTSAKLKKKGINNGADLYKYELKDLIRLLGKA
                     GHFYYYVVRGIDKREVITEFEPKSLSCETTFYEDIDNLDELLIILQQLAERLSNRLIQ
                     KGIKGNNITLKIKYDNFELITRSSNTPSYINKADELFAYGEQLLIANWDSSRKVRLLG
                     LAIGKLDNTNTDEQLFIPL"
     gene            complement(15503..16051)
                     /locus_tag="PLW08_02640"
     CDS             complement(15503..16051)
                     /locus_tag="PLW08_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL51531.1"
                     /translation="MDAQELIFGRHSVRDFLPDPIPIDIINQILEAGRCAPSTQNRQP
                     WRYLVFTENAKIRKLALNCGLIGLSNFFIHKAPCLIIACADLKDNIKINGQDYYLVDT
                     AISFHQMLLYARNYGIESCWLAAFSEKKLKGYLKLPAQWKIVAMAPLGYPAEKKGLYS
                     KAVSGFAKSKDRKPLETIVRFM"
     gene            complement(16033..16557)
                     /locus_tag="PLW08_02645"
     CDS             complement(16033..16557)
                     /locus_tag="PLW08_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPL51532.1"
                     /translation="MKVTAIILASGKGNRFGLPKAEVKIQGQSFLEKIKETLSQADVD
                     DIFTARYKNTTDMLATLRLAVKELKNKQKNSGFLIFPVDFPFVQAKTVQELIKCHKHN
                     PDAIIRPVYQNKSGHPVLLPFLLNLEQDDKKKGLKYIIQNCGLPIFDVPVEDDGILKN
                     INTKEDLELWMPKN"
     gene            complement(16594..18051)
                     /locus_tag="PLW08_02650"
     CDS             complement(16594..18051)
                     /locus_tag="PLW08_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPL51533.1"
                     /translation="MPKLITKFDPIIARKNAKRCKERGIILPTIRQQMFPETIPDEIK
                     EKLQPVGLWDINPLNLFRITWKNDIKTGLFGKPNFLEIPSELSGVKARIIGLVGKYFP
                     TGAHKVGAAYGCLVPRLVTGTFDPEIHKAVWPSTGNYCRGGAFDCALLACPAVAILPE
                     EMSKERFDWLREIGAEVFATPGCESNVKEIYDKCAELRSDPSYVILNQFDEFGNSFWH
                     YHITGNAIAEVFAMVQKPGNRLSGFVSATGSAGTIAAGDFLKKHFPLMKINASEAMEC
                     PTLLMNGFGGHRIEGIGDKHIPWIHNVRNTDIVSAIRDEDCMRLLRLFNEPAGLNYLK
                     KSGIKPELAEKLELLGISSICNLLASIKMAKYFEYNEDDVIVTVFTDSAEMYQSRLQE
                     QTALKGEYTELQAALDRESILQAQSYDNLLELSYWDKKRIHNLKYYTWVEQQGKTYQE
                     ILQQWEPEYWIETFENNLEELDKAIEEFNSLGQSI"
     gene            18567..22358
                     /locus_tag="PLW08_02655"
     CDS             18567..22358
                     /locus_tag="PLW08_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51534.1"
                     /translation="MKKISFILLLVFTTAILVAASGTIKLQDNPAKVQLLEKSDTGLS
                     IAYAVDELQFREINTKEGIFTELNALNYTTTNVTGLPALPLMRQLISVPLGANVTANI
                     VSSSAKVINLDEQGVLYPLMPRQESVSKSANLEQLPFEVNRDFYNANMWTDNPSISVT
                     EIGMMRGTRIFAVDFVPIKYNPVLKKIEVIYQAEVKVAFSGANFIATNELQAKTYSPA
                     FEGVFAQTLINYDEPKVSFLRYPIGYVIILPDNFIAPMQPFIDWKKQEGYNVIVAPTS
                     ITGTTTTSIKAYMQNLWNSATTENPAPSYLLIVGDVAQVPTNNGNAGSHPTDLTYVRL
                     QGTDYMPEMYFGRFSATSVAEVTNQVNKTLMHEQYTMPSDAYLSEVVMIAGVDSYYST
                     THANGQINYGTNNYFNPAHNITSHTYLYPASGSSASAIVQNASNGMGYINYTAHGDVT
                     EWYNPNFSISNINSLQNTNKYSYVVGNCCLTSKFDNPICFAEAWLRATNKGAIIYIGG
                     TNSTYWDEDYWWGVGYKPPIVGTGSPFVAGHTGAYDALFHDHNEPFADWAGNAGSNVV
                     MGNMAVVQSNSSRINYYWEIYSIMGDPSLVPYLGIPAQNAYTPPAQMFLGFNSMDIQT
                     DPYSYVALSMNNELHGVGLADASGNLTLNYTPFTEPGIAKLVITRSLRKPLIANIEII
                     PNEGPYVTVGPLELNDGNNNIAEAGDNINISLQFNNVGIETASNLTATLTCDSPYILL
                     NNATVQLPDIASGNSINQTNAFQISILPDVPDQHLAGFNITVSDGEDTWVTQRSLTIN
                     APNVVFGNHTLFDSNSDGIYQPGETVTISLSISNTGHMNVDGGSLAIVLNSPYAALDN
                     YNFIIPPLTAGNGIPIVLHLFISQDCPNDEVIPIGFAMNAGIQLLNHSIMIPVGIIGE
                     SFESGDFTAFPWVNNSTNPWTIISGTSNAHFGNYAAKSGAIGNNGSTTLQITMEVSSA
                     GNISFWRKVSSEANYDWLKFYIDNNETGSWSGEIGWSEVSYPVTAGSHIFKWTYSKDV
                     GSVSGSDCAWLDDIRFPGMGVLETAIFYTTTTQIEFTNVLPNTTVSVDFALRNLGNLA
                     MQGIITCPPDFELSQYGNALPANYTYQIEAGQTVLYTLSYIAGNSVSEIQTNMQITTN
                     DPHHPVVIIPVNLFPLANDDNVNIPYVTALHKNYPNPFNPETTIHYSLSESGPVKIEV
                     YNIKGQLVRNLVNERKTAGNYTVIWNGKDEQGKNVSSGIYFYRMQTKNYSSTQKMMLM
                     K"
     gene            22471..26157
                     /locus_tag="PLW08_02660"
     CDS             22471..26157
                     /locus_tag="PLW08_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51535.1"
                     /translation="MKKTLFFVILLLSFIGLTAEQIVVSQYPDDIRLTNSNPNNMELE
                     FTLGSFYREPVTINGDSWYHLFLKKEGITLEAGMPQVPVLARSLIIPATAKMHLNITN
                     SEYVELTLPVAPSKGNLTRDIDPATIPYTFADFYQSGESYPAEIAYLTEPFILRDYRG
                     ITVRFQPFIYYPATQTLRVYTKLNISIYAQGTDLTNALLNPKTSYSRYFESTYQNMFL
                     NFSAAKYPSLDEEGRILVIKHSMFDATIQPWVDWKRQLGFTVDVVDVTQAGSTAANIK
                     TYIQNQYDLNNGLMFVQLVGDAPQVPTLTYAGGGSDPSYALLAGNDNYYDIFVGRFSA
                     QTVAELETQVTRSIYYERDIAPGATWLEKAIGIASAEGGGGQGDMGESDITHMNNIRT
                     DLLNYGYTTIDQIYDPGATAAQVSNSVNQGRGFINYCGHGADTYWVTTNFNNNNANAL
                     TNDYMLPLIVSVACVNGNFVSQTCFAEAWMRSVNETTNAPAGAIAFWGSTINQSWAPP
                     MRGQDEVTDLLVGNLKYRVGGLLFNGAHKIIEVYGSQGLADARTWTIFGDASLMVRTK
                     NPQVITATFNPVLFLGMSSFAVQTIPGGRITLTNNGIIYGKGIADATGNCVINLDIIP
                     DLPMDMTLTISAFDYQTYIQTIQVLPSSGPYLVVEETTFSDVNDNIFTTGETVFLDMN
                     LSNIGSETATSVSVTLSTNDSYVTLLNPTLTIGDIDSGNVSSTGTFQIQLSNSIPDEH
                     TVNLHIFIATSDGETFENNCSFIAYAPDITWGPLQINDSLGNNNGRIDAGENVTITFN
                     VTNSGHCDAADISTTLIVNGVSHLITPIISTIENLPVSQTGQMIYNVTFSSQIPMGTS
                     VQLTAMTMFGEYLSVHTYTIVVGIVMENFDFGFSNFPWVFEGGNWTISPDSFNGSMAA
                     KSPSINNNSSTSISITYQCPQAGEISFWKKVSSEPSCDFLKFYINGILKFQWSGTDDF
                     WSQVTYPVSPGTNVFKWEYVKNNGSYQGSDCAWLDDIIFPSTGEQIGIPVFVIDMEDI
                     NFGEVIVNTIVSQTVTICNCGTASMLGTIATESPFSLGEPSMPAYYLEYIIPVGESFT
                     FNVNFQPLQNSVYTGTLIINSDDPNALVNTIPLYGTGQPVANDDPVAVLVTSLKGCYP
                     NPFNPTTTISFSIKEKTPVELIIYNILGQKVRTLVNQPLEPGEHSIVWNGTDNKGRSV
                     ASGIYFYRMKAGNYSETKKMVLKK"
     assembly_gap    26216..26315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26316..26783)
                     /gene="coaE"
                     /locus_tag="PLW08_02665"
     CDS             complement(<26316..26783)
                     /gene="coaE"
                     /locus_tag="PLW08_02665"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424716.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPL51536.1"
                     /translation="MSQNKKPLLIGITGNIGSGKSAFCNFLAANGLKVISADVVANQH
                     LEDPAIKEALIKRYSTAILSPSNEDNGKGIINRKILADVVFSSEQETQYLNSLIHPLV
                     LQDFQKIVEQSNEEALCFEVPLLFEANLQDCFDYIVLISASLETRLMRLEKRGE"
     gene            complement(26770..27516)
                     /gene="cdaA"
                     /locus_tag="PLW08_02670"
     CDS             complement(26770..27516)
                     /gene="cdaA"
                     /locus_tag="PLW08_02670"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPL51537.1"
                     /translation="MEFLIPRFKDIVDILIIAFLIYQALLIVRKSGGYQVLWGLLFVV
                     VLYILAVIFNLKMVGSLLSAIRNYWFIAVVILFQPELRSILARLNLPRELSKVFSKQE
                     KSSLYTPLIDAVSSMSFRKIGALIVLENKRKLHEYIYSGEPIDAIVSMRLILTIFNPK
                     SVLHDGAIIIRDQRIMAAKVVLPLSKKAEYVRKFGTRHLAGIGITEISDAIAIIVSEQ
                     TGQVSVAKAGKIQTNVAFEELLQILTDVTK"
     gene            complement(27517..28350)
                     /gene="folP"
                     /locus_tag="PLW08_02675"
     CDS             complement(27517..28350)
                     /gene="folP"
                     /locus_tag="PLW08_02675"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPL51538.1"
                     /translation="MNKYCFPERIPVLMGILNVTEDSFSDGAQFLDRELAIKHTEVMI
                     AEGAEIIDIGAESTRPGSLPVPAEIQEQRIIPILSSLIEKYPKVTFSVDTQKATVAEK
                     AIELGASIINDISALRTDPALAELLSTNPEVKVILMHMQGTPRTMQINPVYQDVLVEI
                     KDFFQERIDFCLAKGILRENIMLDPGIGFGKNLEHNLTILGNLRTFKEFGLPLVVGAS
                     RKSFIDKISPSSPAQRIGGCLAAAFVSACEEIDILRVHDVLLHRQFFEVLTAIAKAKR
                     D"
     gene            complement(28343..29065)
                     /locus_tag="PLW08_02680"
     CDS             complement(28343..29065)
                     /locus_tag="PLW08_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51539.1"
                     /translation="MRRFQFSKLFNGYFISVAFHLLLFILFAFYVIQPYVPEKWYTFE
                     WEPLDRTTMIETHTANKLEEITMGNNIPGTEGITKQAGISEKKEINTVNGNPVSIGDS
                     EPAIAMPINVLPQDNAKTTIPSSFPHNRNSNALKEVPGTITGSNFGFSSSLEEGGGEA
                     YIISQTKPQINPTEEGEVYLEFKLTPKGTVDMSSVNILSYTSATYAEAVRKVMPSWKF
                     GFKGTYNPKQLYRLRCRFVINE"
     gene            complement(29065..29469)
                     /locus_tag="PLW08_02685"
     CDS             complement(29065..29469)
                     /locus_tag="PLW08_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPL51540.1"
                     /translation="MKMRVSKQRISTTMLISMTDVIFLLLLFLLIASNFVSQTGLPIK
                     LPGSSTAVRQSYQTLLITYYNDGRLYFMDKPIEIKELETVLQQKYQNPEQVVRIAVEK
                     QVPVQKLINLMDIVRNSGYERIFVATTVLEKK"
     gene            complement(29466..30092)
                     /locus_tag="PLW08_02690"
     CDS             complement(29466..30092)
                     /locus_tag="PLW08_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPL51541.1"
                     /translation="MSILDLIVKGGFLMYVLTVISVISIGIIIEKYRQFRKVRLSNNK
                     LQLSLKSQYKLENIRALINMHKSECPMGLMLDKLFNNESDDLDLINQSMEATANLELH
                     RLEKGLGWLSTFAAIAPLIGFLGTVIGMVNVFMKIQAASQSGVDISMLAGGIWVALLT
                     TVGGLIVGIATIIFYNDLVQKLENIVKDMQENAVEYIIKYKKLTQGKL"
     gene            complement(30194..31279)
                     /gene="serC"
                     /locus_tag="PLW08_02695"
     CDS             complement(30194..31279)
                     /gene="serC"
                     /locus_tag="PLW08_02695"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPL51542.1"
                     /translation="MERVHNFNAGPAVLPEEVLREAQADLVNYKGMGLSVMEMSHRSK
                     EFEAIINEARDAAKRIYGLGDDWDVLFLHGGASLQFVMVPMNFLPEGKIANFIHTGVW
                     SKKAMAEVKKLGKPVHIAASSEDKSFSYIPKTYQLSDNPAYLHITTNNTIYGTEWKID
                     PDVPKDVPLIADMSSNFMSKPIDINKYSFIYAGAQKNIGPAGCAVILVKKDFLATGAT
                     NLPSMLDYQLHAKNGSLYNTPPCFTIYMIGLVLKWIENYGGLAKIEENNKAKAKYIYD
                     VIDASNGFYRGTVVPEDRSLMNITFRLPSEELENLFISEAKKNGMIGLKGHRDVGGCR
                     ASCYNALPLNAAYDLAEFMKNFQKQNG"
     gene            31421..32014
                     /locus_tag="PLW08_02700"
     CDS             31421..32014
                     /locus_tag="PLW08_02700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPL51543.1"
                     /translation="MIHELLRHKKVILASVSPRRKELFSLLGISFEAIPAKIEEPINN
                     EAPQTQAMQNALRKAQLVKDKVDKDALVVSADTIVVLDNHILGKPADTEEAGNYLRLL
                     SGRTHSVFTGICIYYNEIANINYEQTFVTFAELSETEIDSYLATGEPLDKAGAYGIQG
                     FGAQFVTKVEGCYFNVMGFPIRLFYEMLKTILVEEEQ"
     gene            32011..>32786
                     /locus_tag="PLW08_02705"
     CDS             32011..>32786
                     /locus_tag="PLW08_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPL51544.1"
                     /translation="MKILANLYLWLNGKFTEETQSLLVDKGIIIEHLPYPVPEQYKNI
                     FQQDFNGAYAYPGFIDTHTHSFEGGLYCSGVDLSKVKNIAEVLELLSAENAKKTKEEI
                     IFAWQLDETLLQEKRFPTVQELDSAVPDKALLLRRIDGHSCMLNSFARKQIKELNTKN
                     EVLKGAENDRAVRHFHSCLTDEEIIEAYQVSAKIGLQGGFTSLHTMIGDAHNSIAHYE
                     LIRDNLDKFPLRFILYPQSFNISAALDAGAKRIGGCILADG"
BASE COUNT        10077 a         6129 c         6117 g        10363 t
ORIGIN      
        1 gttacttaat ctactatata acaagcgatt aagtattatg attcaaagat gttttatttt
       61 tcaatttttt cgttctgatg gttactgtcc ttgccaaccc aaaaagaatt tccagtgacc
      121 gatgaggacc tctttcttcc gattttcatt tgacaattag tcagctacaa aaagatttgg
      181 ctaacgatga ggtgaaattt gaagacagct attattataa aaggtgcgca ggaacataat
      241 ctgaaaaata ttgatttaac tattccgcgt aataatcttg tggtttttac cggcgtatcc
      301 ggttcgggaa aatcgtcctt agcgtttgat actctatatg ctgaaggaca aagaagatat
      361 gtagaatccc tttctgctta tgccagacaa tttttaggcc aaatggaaaa acccaaagtt
      421 gactatattg aaggtctttc tcctgcgata tctatagagc aaaaatcagc cagtaaaaat
      481 ccccgttcca cggtaggaac tgctaccgaa atttacgatt atttaagggt tttgtatgcc
      541 agaatcggaa aacaatattg ttacaattgc ggaagaccgg ttggttctca gacaattgat
      601 cagatgattt cccatttaat gcaaaataaa cccggcacca aactccaaat tttagctccc
      661 attgttcaaa accgtaaagg ggaacataaa gatgaactgg aaatgcttcg tcaggaaggt
      721 tttgtgcgag taatgatcaa tggtcaatta agaaatttgg aagaagaaat tgtattggat
      781 aaaaagagta agcataatat agatgtagtg gttgatagga ttgtaattaa ggaagaaatt
      841 gaaagtcgtc tttccgattc tttggaatta gctttgaaac ttgcagaagg cattgtaaaa
      901 attgactatc cggaagaaaa gcaagaaaca attctttcag ctcaaaatgc ttgcccgcat
      961 tgccatatcg gttttgaaga actttcccct caaagctttt ctttcaacag ccctattggc
     1021 gcttgtcctc tttgtggtgg cttaggttat aaactggaat ttgatccgga taaggttata
     1081 acagaccccg aactaagtat tttggaagga gcggttgccc cctggggtcg tttggaagca
     1141 aaacaacaca gctggacttt aaatacagtc caaagtttag ccagggctta tgatttttct
     1201 ctttccactc cttggtataa attgccggaa atagtaaaac agcttattct ttacggttct
     1261 aaggggaaaa aatttaaaaa tgcctggcag aatgaaaatg gcaggggtga atttatgatg
     1321 cgttttgagg gaattattcc tcagctggaa aggagaatgc acgaaacaac ctctgaagaa
     1381 atgaggcgtt attatcttct gtatattagt gataagccct gtcctgcctg taatggaaat
     1441 aaactgaaac cggcatcgct ggctgttaaa atagccggta aaaatattag cgaaattaca
     1501 gcaatgagca ttcgtgaggt atataatttt tttgataaat tgcagttgaa aggaaatgaa
     1561 aagattattg cggaagaaat tctgaaagaa ataaagaatc gtttgggttt tctgatgaat
     1621 gtcggtttac attatttaac cttggataga aggtctccta ctctttcagg aggtgaagcg
     1681 caacgcattc gtttagcaag ccaaattggc agtcaattag ttggagttat gtatatttta
     1741 gacgagccaa ctataggact tcatcagcgc gatgttgcca aacttgtggc tatgcttttg
     1801 cagcttagag atttaggtaa tactttaatc gttgtagaac atgatgaata taccattaga
     1861 actgccgatc agatagttga ttttggacca agagcaggaa tttatggcgg agaaatagtt
     1921 gccacaggaa ctttggaaac aattttgcaa aacccgaagt cattaaccgg agcatatctt
     1981 tcccgacgct taaaaattcc tgttccggaa aaaagaatta aaccggatga cagaatgctg
     2041 agtatcataa atgcccagca taataatttg aagaatttga atgtggatat tcctttggga
     2101 ctttttgtct gcgttacagg cgtttccgga agcgggaaaa gcagcttaat caatcaaacc
     2161 ctttctcctt ttctgcacaa tcatttttat catactgctc agaaggttgg taaaatggat
     2221 gaaattaaag gatttgaaca catagataaa attattacta tagaccaaca acccatcggc
     2281 agaactccgc gttccaatcc ttctacttat gttaaattgt ttgatcccat tcgtgaactt
     2341 tttgctcagt tgcctgcttc caaaatgcgg ggttacactc ctggcagatt ttccttcaat
     2401 gtaaaaggtg gacgctgtga agcatgcgaa ggtgctggag taaaacaaat agaaatgcac
     2461 tttatggcag atatttttgt aacctgtgag gtctgcaaag gcaaacgcta caatcaggaa
     2521 acactttcta tccgttataa aggaaaaaac attgctgatg tgcttaatat ggatgtgcag
     2581 gaagcaattg agttttttga tgccatacct gctattcgta ataaattgaa aaccttgcag
     2641 gaagtaggat tggattattt gaaactggga cagccctccc ccactctttc cggtggagaa
     2701 gcacaaagaa ttaaactttc ccgggaattg agcaaggttt ctacaggcaa tacactttat
     2761 gttttggatg aaccaacaac cggacttcac tttgatgata tcaacaaact gctgaaggtt
     2821 ctaaaaaaac tcgtagctat gggaaatacg attattgtta tagaacataa tatggatgta
     2881 ataaaatccg cggattggat aattgatttg ggtcctgaag gaggtgatga aggcggaaaa
     2941 ataatctgtt ctggcactcc tgaagaagtt atggcttgtc ctgaatcggc aacaggtctg
     3001 catctaaaac agcaattgga aaaagataag gaagaaacac aaaatctgct actggaaatg
     3061 aattcggaag acaaagcccg taaaaataac ctcaaaaaga aaacaaccaa aagacaggcg
     3121 ctatgagtta tcaacatttt agctattcac ccttaactgc cggaaaacat acagtcggtt
     3181 tagccggtga ttttacttcc tgggaaatta ttcctctgga ggaaattggc ggaatataca
     3241 ctctgtctat agacctgcct ccaggagttt atcaatataa atttatagtg gatggcaatt
     3301 ggattcccga taaaaacaac cctcatcagg tttctgataa ttttgggggt gtaaattctc
     3361 tgctgattgt ggaagaagaa aaagaagaag taacttggga agatattctt gcacaactgc
     3421 caaacaaagc ccctgaaaaa ttctatcagt tctttcgttc agatgtaaat aactatgaac
     3481 tgcgtttttc ctggtatccc aaattagcag aaacaataaa tcttttaacc gaatcttgga
     3541 atatagaatt caagcgtatc ggacaaaatc cgctttatga ggtcttttat tgtttgttta
     3601 aacaaacagg tattttctcc ttccgcataa gaattcaata tgaaaataaa gctctatatt
     3661 ttggtgctga gggcttttcc gaaaaggaag aagatatctc tcccctaaaa ataaacctaa
     3721 aagatattcc tctgtttgca attccggatt gggttagccg cagtattatc tatcagattt
     3781 ttcccgaccg tttttataac ggcaataaag ctaacgatcc cgattttagt gaatggtatt
     3841 atgccgattg taaagaaccg cctcctgatg gagaaaacct ttctccggaa aaagaatatt
     3901 atcatttagt aagtgactgg aacgatatta gcggattaaa acaaagtccc tggcaaaaaa
     3961 aagggatacc ggattttttt tctttttacg gaggagatat tgcaggagtg cggcaaaagc
     4021 ttgaatatct acttgatttg ggcattaatg ttatttattt caatcctttg tggcaggcaa
     4081 aatccaatca taaatatgac agcgcggatt accatagtat agatcctcat tttgctacta
     4141 cggaagaaat gatggatttt gtaaaaatag ctcaccaaaa aggaataaga ataattctgg
     4201 atgtggcttt caatcatacc ggagagacct tttgggcttt tcgggattgt gtggaaaaag
     4261 gacctcaatc accctactgg aattggtatg actggaaaaa atggcccctg ccaaaacccc
     4321 ttcctccaga ttttaatccg aaagagtatt atcaatgttg gtggggaata aaagatatgc
     4381 ccgatttgaa ttatgacctt gctcttcccc atcccgacga aaatgctgtg cgtgatataa
     4441 gaaaagctcg tcctaatgcg cctttggttg attatttaat ttctaccgtt cgctggtggc
     4501 taattgatat tggaattgat ggtttccgac tggatgtgcc ggatgaagtt cccttttggt
     4561 tttgggaact tttccggaat gaagtaaaaa aggcaaaacc ggatgcctgg attgttggag
     4621 aaatatggca tgaagcaaga caatgggttt ctcctttata ttttgattct gtaatgaact
     4681 atgccttttt taaaactccc gtaattgagt tcttcgtgtt aaatatcatc agcaaagagg
     4741 aattttgcca aaaaatagaa accggacttg gtctttatcc ttttcattca ctgaaagcaa
     4801 tgatgaatct gcttggcagt catgatacac ctcgtatttt tgaaatttgc cggggaaatg
     4861 tgcaacgcct taaattggct gttatctttc aaatgtgttt tatcggagct ccccatattt
     4921 attacgggga tgaaattgct atggaaggtg gaaaagatcc tgataacaga agacctttta
     4981 actggaattg ggaagataat cctgttgcct gcgaagtaag ggctttttat aaacagataa
     5041 tttctttaag gaaagctcat cctgcttttt gggatggtca ttttgctttc ttgcctgctc
     5101 ctgaaaatgc tattgctttt ttacgcgagg gagaaagtga aaaaatattg gtattcttga
     5161 atttgggtct tgatactgtt actataagta ttcctgacga gggtgaaatt ctcttcggaa
     5221 atatagatac taaaaaacag ttgcaaactc tttccgctgt aattctttct ttacctgtgc
     5281 agccggagaa ctcgtgacag cttaaaaaac acggatttaa atgaataaaa cagattaata
     5341 ggaagttata atttatagtt cttcattttt ttcggtgttc tctgcgttct tagtgcataa
     5401 taaaatccgc gtaatctgcg tataaaattt tttgccctca cgagtcataa acgccaataa
     5461 aaatatttcc cagctaaaaa aagttataaa aacgcttaat gactcctaac ttaaaagcgt
     5521 taataactcc tgactaaaaa aagggcagaa aacaagaaat gtcttctgcc ctgtttgtaa
     5581 aggaattgaa ctatttggct tttttaactg cctctttaag gtttttacca gctttgaaaa
     5641 ccggaacatt gcgtgccgga atcttcaaag gttttttggt tttgggattg ataccattgc
     5701 gcgctttacg atgtgcaacg ctaaaagaac caaaaccgac taaagaaact ttttcaccct
     5761 ttttcaagga ttccgttatg ccctcaagaa cagctgtgag ggctgcactg gctacttttt
     5821 gagaagtacc agcattagct gcgatctttt ttaccaattc gtctctgctc attttacctc
     5881 cttatttggt ttagtgtaaa gagtaggaca tctttcaatt gtaaacaaag tgcattccga
     5941 aaattctgtc aataaaaaaa tgcttatttt tcgttttttt tacgaaatta gaggtttcag
     6001 catagttcct aatatgcgta acgctatata atatagtaag ttagtgcatt attgcaaggg
     6061 caaaaaatta tttgtaattt ttcttttgtt ttgggcttta tactttttta agttgacaaa
     6121 aatcagacca atgagggcat agttaataaa aaaatctgga ggttttacca atgaaacaga
     6181 aaataatgac cttcgtagtc cttgttcttt tggtgttgct tagtgcctgt ggcagcaaca
     6241 aaaaagttca agcacccgtt gaaccggtcc gaaatcccct aattttagct cattcagcag
     6301 ctgcaagagg aactgaggca ttcaacgatg aattttataa tgatgctatt tctgctttta
     6361 cagaagcaat atccttgttt aatgaagctg cgctaactgc tactcctgcc gattcagttg
     6421 ccgagaacat tgaaaagatg aatttgaaca ttgccaagtc ctatatagaa cttgccaatg
     6481 aaagtatgga attaagcagt tttgatgaag cattggatta ctatagcaaa gccctgaatg
     6541 tttataagtc aatcactccc aaactaataa cccaggaaga actacaacag aacataattg
     6601 gtatctataa taatatggct attacagcta aacaagcagg taaatatgaa caggcgctaa
     6661 cttattacga tcagatattg aaactcactc ccaacgatgc cgatgttctt tatgccaagt
     6721 tttatgtttt acgggataat attaaggatg aaaatcgcgc ttatgctgtt ttaattgact
     6781 atgcagagct cactcaggat gccaaagcat ttatcaatgt agcagatagc tatgcggaaa
     6841 aaggcaatct gattgaagcc ggaaaatatt atcagaaagc attagctatt gaacccagcg
     6901 ttgatcttta tcgtcgttta gcaaattact atagcggttt gaaggactat aataatgcga
     6961 acctttattt gcaaaaggta gcggactcca atcccgaacc ctctgagctg gcacaaatat
     7021 acgctctgat ggctaaaaat tatgagggaa tgaatgataa gaaaaagatg gtggaatatt
     7081 acgaaaagtc cttaagtata gaacgcaatc ctcaactggc attgctttta gcaagttatt
     7141 acaattctca aaagaattat gccaaagtaa tcagcaacgc caatatcacc cttagcatag
     7201 accccaaaaa tgctgatgct ttaatgttgc gtggtgttgc ctattatcag atgaagaatt
     7261 attctgctgc taaagctgat ttggaacggt tgcaaaacga tcctaaatat ggaactcaag
     7321 ctaagaattt gctgaagaat atacctaaat aactaaatct taaatgcagg acgggatttg
     7381 aattggcaac ccgtcctgcg ttctgaaaaa acaatgaaat tacatagtga cggctcaata
     7441 aaagctattt attccgctac tcaagggaaa aaacaatatc gtcccttgat gagttccaaa
     7501 attcctgctg gttttccttc tccggcacag gattatattg aaggcaagct ggatttgaat
     7561 gaatatttga tagttcattc tgcctcaact ttctttgtaa gagttgatgg ctattcaatg
     7621 cagggtgcag gaattatgcc tgatgatatt ctgattgtag atagagcatt ggaggcaact
     7681 aataaccgca tagtaattgc tattgtggat ggtgaattaa cagtaaaacg actgaaaatt
     7741 aaagggaaaa aatattattt agtgccggag aatgatgaat ttgaaccaat tctcattcag
     7801 gaaggaatgg acttcattat ttggggtgtt gtaacctacg ttatccataa agtttaatca
     7861 ggtggcaata aacaagccct gtttctttag tttgctcttt attcttggaa ttactacact
     7921 gtttgctcag tccctggtta tagaacataa tcttgaatta gaacctaatc gctataaata
     7981 tcctctggca aattcaccta taattaagaa cagcgtttcc gtttgggctg attcattgct
     8041 attagtggaa ggaacagatt attgggtaaa tcatcagaag gcagaattga ttttgcttac
     8101 acaacccgaa gttcctgttc taaaagtgga atatattctt gtgccggaat ttttaactgt
     8161 tcctttgcaa aattggaaac ctcaagaata tagtgatacc ttgctaactt ctattaaaaa
     8221 gcgtaaaaat cctattctgg cagaagatgc ccgtttggat attcaaggca caaaaacatt
     8281 tgccatcact ttttccgatg aatctacttt tgatttgaaa caatctctgt ttgtaaacat
     8341 ttccggtgag cttgcccaca atgtttacat caatgctcag ctttccgata gccaatcaaa
     8401 actttctcct gaaggggatt ccaaggaatt gagttccctg gataatgttt tcatccgtat
     8461 ttatggtcct aaatatgaac ttgcaatggg tgatttgacg ctaaaataca ccgggacacg
     8521 ctatatggaa tattacagca aatttgaagg gctaaatgct tggctgaggg gtaaacatta
     8581 tgtccaaacg gctttctctg caggaggagg taaaaatgcc tccgtaaaaa taacgatcat
     8641 agacggaaaa cagggtcctt attatttaag agctaatgat acccaatcca attttattgt
     8701 cgttgccggt agtgaagaga tttttgtgga tggcagtaaa tgggaaaggg gaattgatta
     8761 cagtattgat tacagcgaag gcagtgtaat gtttaaacat cttattagct cttccaataa
     8821 tgtattggca cgatttcaat attccgatga atattatcct caaagcagtt atctcaattc
     8881 ttctcaatta cagcttgcag aacatttaac gctgacacat cattttatct ggcaacaaga
     8941 tgataagaaa aatcccttgc tctatgaatt cagttctgcc gatttggatt ccttaaaatc
     9001 tgcaggggat aattttgttt ggggtgaagg catttattta gttgaagctg gaacaggtga
     9061 ttataaacta attcagttgt cccaaattga atattatgaa tatgctccgg gtgattctct
     9121 tgcctgttat aatattgttt tcagctatgt tggcagtgga aacggtgatt atgaagaata
     9181 ttctccgggg aaatatcgtt atttaggttc aaaaatgggt tcttggctac ctcaaaaact
     9241 tttggtgccc cctgaaaaaa agggaaatct ggatttggct gttgcttatt ctaaagataa
     9301 tttgaatgct ggggttgaag ttttaggcag tgtaaatgat cgtaatactt tttcggtaaa
     9361 ggatgatgag gataatgatg ccggtttgct ttatgcttat ttggaagttc ttttttatcg
     9421 ttattctttc aggttggaac acgaacaacg cagtgaaaaa acttatctct ttggtaaata
     9481 tcgcaatccc gatgtagaat atgatttcgc agctatagaa acagccgatt ctttagcaca
     9541 gcaggaaagc aatattcaaa taagtagtaa gcaaacaaat tggaatgtat ctctcctttt
     9601 gcgctaccgt aggatttatg atttatacac tttacaagca ttgcgtttca cttcttcctc
     9661 ttcaggaaaa ggtttgctgc ctgccataaa tttacatagt actctttctg cacaggatta
     9721 tgctaatgac gatgttaggg atggaacttt gcaatatcat cagggagata tttcctgggc
     9781 ttggaaaata ttgcgcgtgc gttttgagac actatataac cttttggata caaaaaatta
     9841 tgggaacagc tatctgaaag caaaccccgg aatcactatc ggaaatccct catcctttct
     9901 tacccagctt tcctatacaa cagataaaac caaactccag gaaaatgatg cctggaacga
     9961 aatttccagt tctaatacct acgccttaaa acaaatgatt aaccttggtg aacagcgtat
    10021 tgacctggat gtaacacacc gtgaagtcca acaaccgtat tccacttcca atcccaaatc
    10081 caattacgac ctgatcaatt tccgttccag caatagcttt ctgaaaaatt ctatcggctt
    10141 ttacagtaac tatcaactca atcaaaccga attttatccc aaaatccgtg aattgcaata
    10201 tgtgggttcc ggtttgggtt attatgacag caccggggta agtgttacgg atggtgatta
    10261 cgattgggtt tatattacaa gcagcacggg aagtttatca tcagaaataa attgtctgct
    10321 aaacctttac tggaaaccgg gaaatgtctc ttccagggat tttataaagc gtttgcagag
    10381 cgatacctcc ataaatttga ttgaacaaag ttttcaacgc aataattgga aaagctattt
    10441 ctttttaccc ggatcagtat acaatgaaga aacaacaatt tacggcagac aaaatataga
    10501 agaagttctg tggctggatt tgattcgtaa taaacttact gccaatcttc agatttcaat
    10561 ggaccgaact ttggataaaa gatatcaaac accggataga actttcagtt tctcccaagt
    10621 cgctcaattt gatattaaag ggtttaacgc ttataatacc cgtctacaat tcagcaatga
    10681 gaccaaccgc gatacacatt atttaagcga aattgatatt ataagtgcca gtgctttagt
    10741 tcagcgtaat ttgaatccgc aaagcaatat tcaggcagaa ctgagttgta gttcggaatc
    10801 aggaggaaag caagatggca gtgaaaatta tcgtctgtat tcttttgctt tttctccttc
    10861 cctgaaaagt gtatttatgc aaaaataccg cctttctgct tctctggcaa taacttataa
    10921 tcgcctttcg ggaagcgagt atttttcctt tctaccccaa aaaagagcgg gctggattcc
    10981 ctctgcaaat ttaagcggtg tttatagaat caatactttt tgccttatta ccctggatta
    11041 tcgtttttcg gattatccaa aacaatcaag ttcgcatgaa ttaaaactgg aatttaaggc
    11101 ggaaatataa gttatgcaaa gtattatttt aagtcctctg gattatatta tcgtcctgcc
    11161 ttttattttt cttgccggtt ttattgattc catagctgga ggtggaggtt taatttcttt
    11221 acctgcttat tggagtgtcg gaataccacc tcatttggct ttaggcacta ataaattttc
    11281 ttcctgttgt ggaactcttt tttccactgc caattatttc aaagctaaaa tgattgatat
    11341 tcctgttgcc ttagtaagtg ccggaatggc tataattggc tcttggcttg gtgcttcaac
    11401 agcattaagg gtttcctcac aagtgctcaa ttatttattg attatcctga ttcctgcagt
    11461 agctattttt tctttgttga acagaaatct ggggatgcaa agcaaagcag agtttctggc
    11521 aaaaagattg cgtatgctgt taggaggaat agcgggactg ggaattggct tttatgatgg
    11581 attcttcggt cctggaacag gaacttttct gattttaatt tatacttccc ttttacacta
    11641 tgattttgta accgctaatg gtaataccaa agttgtaaat ttggcttcca atctatctgc
    11701 attgattaca tttgcctttg cttccaatat ttattacccg cttgctgttc caggtgcctt
    11761 atgtggaata gcaggaaata ttctgggttc taaactggtt attttaaggg gcaataagct
    11821 aatcagaaat gtttttattc tcgctttact tttacttttt gcccgagtaa tttataatct
    11881 tgtgtaacaa aatgaacgat gcccctattg ttcatttata agttaggagt cgtcattgcc
    11941 ttcaagaatt ttacatatcc cacaaagtta tttaacaata aggactcgtg acttaatttg
    12001 gttgaagagg actacaacct tcctgaaaca attatcttga ctttttggca cattcaattt
    12061 ttttgtgcaa aaagaaataa atccgaggag aaaagtggat gaaaaagcaa ataatcatct
    12121 tctgcctaat gcttttagtg ctaactgcct gttctaccgc tcgtcaaaaa cttagtcccc
    12181 agggaaatgt taatctgaaa actgccgatg tatattattc ccaacaagat gtggaacaag
    12241 cggaaatata ctatttgaaa gtccttgaag ataatccgga tcacgctatt gccttaagac
    12301 gcttgggtga tatcagcttg tttaaagcgg aaaatttttc agcccgggaa gttgaatttt
    12361 acgaagatgc ctaccattat tatgccaaag caattagcgt tactgaacaa tttcccaatc
    12421 ttacggatca ggatcgtatt gacctgagag atatgaaaaa aaggaaagaa agagcttgga
    12481 ccaggatttt cctggctgca ggcaaggaaa aagaagcagg aaatacacaa cgcgctatgg
    12541 aaatttatga gctggcacat aaactggaac cagaacgtcc tgaaccaatg atccagctta
    12601 aaaatatcta tctggttgag cttaaagatg atgttaaagc cgaacaaatt cttcagcaat
    12661 tgttacagaa agatcctgat aaactggaat atttaatgga aatgggaacc ttctattaca
    12721 ataagggtaa ctatgctgaa gccgtaaaat actttgaaaa agcaagacct caaattccta
    12781 caaatattga taatttgatg aatatctctg cctgctacta tgaactgaaa gattttgaaa
    12841 aagctatgtc cgccactaaa actgctttgg aaatagaacc aaataatgtt gatcttttag
    12901 ataatgctcg ttctattgct gctcaactta atgatataga tcaggcaata ttctatctta
    12961 aaaagttatt ggatatccgt ccgaatgagg atgatttcag tttgcttgcc acctatcttt
    13021 acaataaaca aaattggcag gaactgatca aatatgctga acagtggtat aattgggata
    13081 aatccaataa aatagccgtt gaatacatta tctgggctgc tcaacaaacc ggaaacaaac
    13141 aattggaaac caaatatact gccatcaaaa aaactatgcc ttaattgttt tgggttggtt
    13201 gaaaatgtag gaatagctgg aggataccgt tcctccgcaa gaagttttta agatatagat
    13261 cagcgatatc tatataatct gcttgagata tttttgtgct gtcacgagtc ataagcgccg
    13321 tataatttat ttcaggggtt acaattatta aggcgctaat gactcctaac taatatagca
    13381 aatgacttcg gacaaaatat gagccaaagg aatttattta atgtccaaac aaaataaggc
    13441 atatttgttt gccggggctt ctattcttgc ctggtcaaca atcagcacgg catttaaatt
    13501 ggctttaaat tatttaaccc ctgtgggatt gttatttttt gcttctttaa cggcttttct
    13561 ttttctggga attgttaacg cagttagtaa cccttctctt tttaagacga aggagttgtt
    13621 taaaacagta ggcaggaatc tgaaaatttc ttttctgcgg ggattactta atccttttat
    13681 ctattattta atgctttttg aggcatattc ccgtttgcga gctcaagaag cgcaagcgtt
    13741 aaattacact tgggcaattg tattagcgtt attcagcatc tggctgttga aagaaaaatt
    13801 tcggattatt gattttgttt ctttgcttat cagttttttt ggggtttggg ttatttctac
    13861 aaaagggcag attacttcct tacagtttga tgatgcctta ggttcatttt tagccgtttc
    13921 aacttctatt atttgggcat tttactggat tttaaatatt aaggataaaa ggcctgctct
    13981 tacgaagctc aattacaact tttttctcgg ctttttgttt atagctcttt ttgctctttt
    14041 tacgcataaa tctcttttta cgccccaggc aatttacggt tacggcattc tgggaggtat
    14101 ttatgtaggc atttttgaaa tgggtttaac ttttctgctt tggaataaag cccttgaatt
    14161 gaccgataat acggctgtta tttctaatct gattttcttt actccttttt tatcgctact
    14221 ttttatcagt tccgtattaa aagaaagtat ccactatgct acttttattg gacttttgtt
    14281 aattttcttc agcaacctgt tacagaaagg tttaccggct atattaaagc ggaatgaata
    14341 attgttcatc cgtattagta ttgtccagtt tgccaatagc caaaccaagg agacggactt
    14401 tccgggaact atcccaattg gcaattaaga gctgttcgcc gtaagcaaat aattcatcag
    14461 ctttattgat ataagaaggt gtattggaac tgcgggtaat cagttcaaag ttatcgtatt
    14521 ttatttttag ggttatgtta tttcccttaa tgcctttttg tatcagacgg ttggacaagc
    14581 gttctgcaag ctgttgtaaa ataattaaaa gttcatccaa attatcaata tcctcataaa
    14641 aagttgtttc acagcttaat gatttcggtt caaattcggt tatcacttcg cgtttatcaa
    14701 taccccgaac aacataataa tagaaatgac cagctttccc taataatctt atcaagtcct
    14761 tcaattcgta tttatagagg tcagctccat tatttattcc ctttttcttt agcttggcag
    14821 aagtaacttt accgatgccg taaaatttat caatgggcag agcaaaaaga acttcgcttg
    14881 ccttttccgg aggaatataa gttaaaccat ccggtttatt caaatcactg ccgattttag
    14941 cgaggaattt attgtaggaa actccggcag aacaggttag tcccgttttt tccaaaatct
    15001 ccgctttaat taatttggct attttaacgg catcatcttc gcccaatttg ttttcggtaa
    15061 catccaaata tgcttcatcc aaagaaagcg gttctaccaa atcagtccat tgataaaaaa
    15121 tttcctttat ctgatgggag acctttttat ataaattaaa gtgggggtga ataaaaattg
    15181 cctcaggaca caaattccat gcctggtaac tgctcattcc actatgaact ccgaatttgc
    15241 gtgcttcata agaacaggtg gaaacaaccc ctctgctatt tggaggtcct ccaacgatta
    15301 cacatttgcc tttcagccgg ggattttccc taatttctat tgccgcaaaa aaggcatcca
    15361 tatcaatatg aataatcttc ttcataactc tttgagaaag aatggggtgg caaatttctt
    15421 tttgctacaa gtaacggaat tagataaaag caatttatac ttcgtctttt gccgaatcta
    15481 aattcggtga atagaagggt ttttacataa aacggacaat tgtttccagt ggctttctat
    15541 ctttactttt ggcaaaaccg ctaaccgctt tggaatataa tccttttttc tcagcaggat
    15601 atcctaaagg agccatagct acaattttcc actgagcggg aagttttaaa taacccttca
    15661 gtttcttttc ggaaaaagca gccagccaac aactttcaat gccatagttg cgggcatata
    15721 atagcatttg atgaaaggaa atagccgtat ccaccaaata ataatcctgt ccgttaattt
    15781 ttatattatc cttcaaatcg gcacaggcaa taattaaaca gggcgcttta tgaatgaaaa
    15841 aattggaaag ccctatcaat ccgcagttta atgccaattt ccgaattttg gcattttccg
    15901 taaaaaccag ataacgccag ggctgacgat tttgagttga aggagcgcat cttcccgctt
    15961 ctaaaatttg gtttataata tctataggaa tagggtcggg taaaaaatcc cgtacactgt
    16021 gtcttccgaa aatcaattct tgggcatcca taactccaaa tcctcttttg tatttatgtt
    16081 ttttaagata ccatcatcct caaccggtac atcaaatatc ggtaaaccgc agttctgaat
    16141 aatatatttc aagccctttt ttttatcatc ctgttccaag tttaacaaaa aaggtagcaa
    16201 aacagggtgt ccgcttttat tttgataaac ggggcggata atagcatccg gattatgttt
    16261 atggcattta ataagttcct gaaccgtttt tgcctgcaca aaaggaaaat ctaccggaaa
    16321 aataaggaaa ccgctatttt tctgtttatt tttcaattcc ttaacagcca agcgtaaagt
    16381 ggcgagcata tcggtagtat ttttatatct tgcagtaaaa atatcatcta catctgcctg
    16441 ggataaggtc tccttaatct tttccagaaa gctttgacct tgaattttca cttcggcttt
    16501 aggcaaacca aatcggttcc ctttacccga agcaagaatg atagctgtca ccttcataaa
    16561 attctctcag ccctaatctc tttgccctcg gctttatata ctctgtccta agctattgaa
    16621 ttcctcaatt gctttatcca attcctccag attattttca aatgtctcaa tccaatattc
    16681 cggttcccat tgctgaagga tttcctgata tgttttacct tgttgctcta cccaggtata
    16741 gtattttaga ttatgaatcc gctttttatc ccaatagctt aattccagca aattatcata
    16801 actttgtgcc tgaagaattg attctctgtc taaagctgcc tgcaattcag tatattcgcc
    16861 ttttaacgct gtttgttcct gtaaacggga ttgatacatt tcagcactgt ccgtaaaaac
    16921 cgtgacaata acatcatctt cattgtattc aaaatacttt gccattttga tagaagccag
    16981 cagattgcag atggatgata tgcctaaaag ttccagtttt tctgccaatt ccggttttat
    17041 tccacttttc tttaaatagt ttaatcccgc cggttcattg aataaacgca aaagccgcat
    17101 acaatcctca tctctgatag cgcttacaat atccgtattc cggacattat gaatccaggg
    17161 aatatgctta tcgccgattc cttcaatccg atgaccgcca aaaccattca ttaataaagt
    17221 gggacattcc atagcttccg aagcgtttat tttcattagg ggaaaatgtt ttttcagaaa
    17281 atctcccgct gcaattgtcc ctgcactgcc tgtagcgcta acaaatccgc ttaaacgatt
    17341 gcccggtttt tgcaccattg caaagacctc agcaatagca ttaccggtaa tatgatagtg
    17401 ccaaaaagaa ttgccaaatt catcaaattg gttcaaaata acataacttg ggtctgaacg
    17461 caattctgca catttatcat agatctcttt tacattggat tcacatccgg gagtggcaaa
    17521 aacctctgct ccaatttcgc gcaaccaatc aaaacgttct ttgctcattt cttcaggtaa
    17581 aatagcaact gccgggcaag ctaaaagagc acaatcaaaa gcaccacctc tacaataatt
    17641 tcctgttgaa ggccaaacgg ctttatgaat ttcgggatca aaagttcccg ttaccaaacg
    17701 cggaaccaaa catccgtaag cagctccaac tttatgagct cctgtaggaa aatattttcc
    17761 taccaaacca ataattctgg ctttaactcc ggaaagttct gaaggtattt ccaaaaaatt
    17821 gggcttgccg aagagtcctg tttttatgtc atttttccaa gtaatccgaa aaagatttaa
    17881 gggattgata tcccaaagtc caacaggttg aagtttttct tttatctcat ctggaatcgt
    17941 ttccggaaac atctgttgac gaattgtagg aagaattatt cctctttctt tacaacgctt
    18001 tgcatttttc ctagcaatta tggggtcaaa tttcgtaatc aatttgggca tttatatctc
    18061 cttctttaac aatcaataga aagaaccaaa aataaaagaa gggttttttg taaaggaatt
    18121 tacaccgaaa ccaaataatt tctcccagct caaaaacttt aatgcagatg aaaattgtgg
    18181 actaccgatt ttctttttca ccaatacaaa tttaactgtt attatagttt ccttttacct
    18241 ctgcatcaat gctaacattc atattcttat gatgttacaa aacaattttc agttcttcct
    18301 taatagcatt gcttacccat tgcttaccca ttactaatgg cattagcaat gagtccgtaa
    18361 tgcttaagca atgctattaa gctaaataag ttaactcatt tatttattaa aatgttagcg
    18421 aatattcaac aactcagata agatttattt tggggtttcg tcctctaaaa ttacaatatt
    18481 ttactgtttt cggcttcatt cttgcttata ttatatgtat aggcagtaaa atatataaag
    18541 ataagaataa tataaaggga gaagtaatga agaagatttc tttcatactc ttactggttt
    18601 tcacaacagc tatcttagta gctgccagtg gaaccatcaa attacaagat aatccggcaa
    18661 aagtgcaatt gctggaaaaa agcgatacgg ggctttctat cgcttatgct gtagatgaat
    18721 tacagtttag agaaattaat accaaggaag gtatttttac tgaactaaat gccttaaatt
    18781 acactactac taatgttact ggcttaccgg cattaccttt aatgcgacag ctgattagtg
    18841 ttcccttagg agcaaatgtt actgccaata ttgtcagcag tagtgctaag gttattaatt
    18901 tggatgaaca gggtgtttta tatcctctaa tgccgcgtca ggaatcagtt tccaagtcag
    18961 caaacttgga gcaattacct tttgaagtaa acagagattt ctataatgcc aatatgtgga
    19021 ctgataaccc ttccattagt gtaacggaaa tcggaatgat gcgcggaaca agaatttttg
    19081 ctgttgattt tgtacctata aaatacaatc ctgttctcaa aaagatagag gtaatttatc
    19141 aggcggaagt taaagttgct tttagtgggg cgaactttat tgctaccaat gaacttcaag
    19201 caaaaacata ttctcccgct tttgaaggtg tatttgcaca aacacttatc aattatgatg
    19261 aaccaaaggt ttcttttctc cgctatccga ttggctatgt aatcattcta ccggataact
    19321 ttattgctcc aatgcaacct tttattgact ggaaaaaaca agaaggttac aatgtaattg
    19381 tagcgcctac ttctataacc ggaacaacta caaccagcat aaaggcatat atgcaaaatt
    19441 tgtggaattc agctacaacc gaaaatcctg ctccttctta tcttcttatt gtaggtgatg
    19501 ttgctcaggt tcctaccaat aatggaaatg caggttcaca tcccactgat cttacttatg
    19561 ttcgtttaca gggaactgat tatatgccgg aaatgtattt tggtagattt tccgcaactt
    19621 ctgtagccga agtaaccaat caagtgaata aaaccttaat gcatgaacaa tatacaatgc
    19681 ccagcgatgc ctatttaagc gaagtagtta tgattgctgg tgttgatagt tactattcca
    19741 ctacccatgc caatggccag attaactacg gaactaataa ttattttaat cctgctcata
    19801 atattaccag ccacacttat ctctatcctg catcgggaag ttcggcatca gcaattgtgc
    19861 aaaatgcttc caatggaatg ggctatataa attacactgc gcatggtgat gtaactgaat
    19921 ggtataatcc taacttttca attagtaata tcaactcttt gcaaaatact aataaataca
    19981 gttatgtagt gggaaattgc tgtttaacta gtaaatttga caacccaata tgctttgctg
    20041 aagcatggtt acgtgctact aataaaggtg ctataattta tatcggaggg acaaatagca
    20101 cttattggga tgaagattac tggtggggag ttggatataa gccgcctatt gtaggaactg
    20161 gctctccttt tgtagcaggt catacaggag cttatgatgc tctctttcat gatcataatg
    20221 aaccctttgc tgattgggct ggaaatgccg gaagtaatgt tgtaatggga aatatggcag
    20281 ttgtgcaatc caatagtagc cggataaatt attactggga aatttattct attatgggtg
    20341 atccttcttt agttccttat ttgggaattc cagctcaaaa tgcctatact ccacctgctc
    20401 aaatgttttt gggtttcaac agtatggata ttcagaccga tccttacagc tatgtagctc
    20461 tttctatgaa caatgaattg catggcgttg gtttagctga tgccagtggt aatttaactt
    20521 tgaattatac tccttttact gaacccggaa tagcaaaact ggtaataacc cgttccttgc
    20581 gtaaaccctt aattgccaat atagaaatta tacccaatga aggaccttat gtaactgtcg
    20641 gacctttgga attaaatgat ggaaataaca acattgctga agccggtgat aatataaata
    20701 tttccctgca attcaataat gtaggaattg aaaccgcttc taatctcact gctaccctca
    20761 cttgcgatag cccttatatc ttgttaaata atgctacagt ccagttgcct gatattgctt
    20821 ccggcaacag tattaatcaa acaaatgctt tccagataag catcctgcct gatgttcctg
    20881 atcagcatct tgcaggtttc aatattactg ttagcgatgg tgaagatacc tgggtaactc
    20941 aacgcagttt aactatcaac gctcccaatg tagtttttgg taatcacaca ttgtttgata
    21001 gcaatagcga tggaatttat cagccaggtg aaacagttac aatatcctta tctatttcca
    21061 ataccggtca tatgaatgtt gatggtggtt cattagctat tgttttaaac agtccttatg
    21121 ctgctttgga caattacaat ttcatcattc ctccgcttac agcaggcaat ggtataccta
    21181 ttgttcttca tttgtttatc agtcaggatt gtcctaatga cgaagttatt ccaatcggat
    21241 ttgctatgaa tgcgggaatt caactattga atcatagtat tatgatccct gtcggtatta
    21301 ttggtgaaag ttttgaaagc ggtgatttta ctgctttccc ttgggtaaat aacagcacaa
    21361 atccctggac tattattagt ggcacttcta atgcccattt tggtaattat gctgccaaat
    21421 cgggtgctat aggaaataat ggcagcacca ctttacagat aactatggaa gtttcttctg
    21481 caggaaatat ttcgttctgg cgtaaagtaa gtagtgaagc aaattacgat tggctcaaat
    21541 tctatattga caataatgaa actggttcct ggagcggtga aattggttgg tcggaagtaa
    21601 gctatcccgt taccgctggt agccatattt tcaagtggac atattctaaa gatgttggat
    21661 cagtttcagg tagtgactgt gcctggttgg atgatatccg tttcccggga atgggagttt
    21721 tggaaactgc aatattctat actacaacta cccaaattga gtttaccaat gttcttccca
    21781 atacaaccgt gagcgttgat tttgccttgc gcaatttggg taacttagca atgcagggaa
    21841 ttattacctg tccccctgat tttgaactta gtcaatacgg caatgcttta cctgctaatt
    21901 atacctacca gattgaagct gggcagacag tgctctacac tctcagttat attgctggaa
    21961 attcagtgag tgaaattcaa accaatatgc aaattacgac taatgatcct catcatccgg
    22021 tggtgataat acctgttaat ttgtttcctc ttgccaatga tgataatgtt aatattcctt
    22081 atgtgaccgc actgcataag aactatccta atcctttcaa ccctgaaaca accattcatt
    22141 actcacttag cgaatcagga ccggttaaaa ttgaggtcta taacattaag ggtcaattag
    22201 tacgcaatct tgtaaatgaa cgcaagaccg caggcaatta tactgttatc tggaacggaa
    22261 aagatgaaca gggtaaaaat gtttccagtg gaatctattt ttaccgaatg caaaccaaaa
    22321 actattcttc tacccaaaaa atgatgttaa tgaaataaat accgtttgaa gcaggcattc
    22381 ttattgaaag gatgcctgct tcacggaaaa gagagttcaa ataatgaatg attatcctta
    22441 cattttagtc agcaataaag gaaggaatct atgaaaaaga ccttattctt cgtaattctg
    22501 ctgttgagtt ttattggctt aacagctgaa caaattgttg ttagtcaata tcctgatgat
    22561 attcgtttaa ctaacagcaa tcccaataat atggaactgg agtttactct gggcagtttt
    22621 tatcgcgaac ctgttacgat aaatggagat agttggtatc atctcttttt gaaaaaggaa
    22681 gggattacct tagaagcagg aatgccgcaa gttcctgttt tagcaagaag tttaattatc
    22741 cctgctacag caaaaatgca tctgaatatt accaatagtg aatatgtgga gcttactttg
    22801 ccggtagcgc cttccaaagg aaatctaacc cgtgatattg atcctgccac tattccctac
    22861 acatttgccg atttctatca gagcggggaa agttatcctg ctgaaattgc atacttaacc
    22921 gaacctttta ttttgcgtga ctaccgcgga ataactgttc gttttcaacc ctttatatat
    22981 tatcctgcta ctcaaactct gagggtctat acaaaactga atatttctat ttacgcacag
    23041 ggaactgacc tcacaaacgc tcttttgaat ccgaaaacat cctatagccg ttattttgaa
    23101 agcacttatc agaatatgtt cctaaatttt agtgctgcca aatatccttc tctggatgaa
    23161 gaaggcagga ttttagttat taaacacagt atgtttgatg ctactattca accctgggtt
    23221 gattggaaaa gacaacttgg ttttaccgtt gatgttgttg atgttacgca agcggggtct
    23281 actgccgcaa atataaaaac ctacattcaa aaccagtatg atttgaacaa tggcttgatg
    23341 tttgttcagc tggttggtga tgctcctcaa gttcctactt taacatatgc aggtggaggt
    23401 agtgatccat cctacgctct cttggctgga aatgataatt attatgatat ctttgtgggt
    23461 cgtttttctg cacagacagt agctgaactg gaaactcaag tcactcgcag tatatattat
    23521 gaacgagata ttgctccggg tgcaacctgg ctggaaaaag caattggaat tgcttctgca
    23581 gaaggtggtg gcggccaagg agatatgggt gaaagtgata ttacacatat gaataatatt
    23641 cgcaccgacc tgcttaatta tggttataca accattgatc aaatctatga cccaggtgct
    23701 acagccgctc aagtaagtaa ttctgtaaat cagggtagag gttttatcaa ttattgtggt
    23761 catggtgctg atacatattg ggtaactaca aatttcaaca acaacaatgc taatgctcta
    23821 accaatgatt atatgttacc tcttattgtt tctgttgcct gtgtgaatgg aaactttgtt
    23881 agccaaactt gttttgctga ggcctggatg cgttctgtaa atgaaacaac aaatgctcct
    23941 gccggagcta tagctttttg gggttctaca attaatcaaa gttgggctcc acctatgcgc
    24001 ggtcaggatg aagtaaccga tctcttagtt ggcaatttaa aatatcgggt tggcggttta
    24061 ctatttaacg gggcacataa aattattgaa gtttatggta gccaagggct tgctgatgcc
    24121 agaacctgga ctatttttgg agatgcatcg ttaatggtga gaaccaaaaa ccctcaagtt
    24181 attactgcta ctttcaatcc tgtcctgttt ttaggtatga gcagttttgc tgtccaaacc
    24241 attcccggcg gtagaattac tttaactaat aacggcatta tctatggtaa aggaattgcc
    24301 gatgctacag gtaattgtgt tatcaatctg gatattattc ccgatctgcc aatggatatg
    24361 actttaacta tttccgcatt tgattatcag acctatattc aaaccataca ggttttaccc
    24421 agtagtggac cctatttagt ggtagaagaa actactttca gcgatgttaa tgataatatt
    24481 tttactacag gggaaaccgt ctttctggat atgaaccttt ctaatatcgg tagtgaaact
    24541 gccaccagtg tgagtgttac tctttccaca aatgattctt atgtaaccct gttgaacccc
    24601 actctcacaa ttggagatat tgacagtgga aatgttagct ctacaggaac ttttcaaatt
    24661 cagctttcca acagcattcc cgatgaacat acggtaaatt tacacatatt tattgctacc
    24721 agtgatggag aaacttttga aaataattgt agctttatag cgtatgctcc ggatattacc
    24781 tggggtccat tacagataaa tgatagttta ggtaataata acggacgcat tgatgccggt
    24841 gaaaatgtaa ctattacctt taatgtaact aattcgggac actgtgatgc tgccgatatt
    24901 tccactactt tgattgtaaa tggagtatcc catttgataa ctcctattat ttccactatt
    24961 gaaaatctgc ctgttagcca aaccggtcag atgatttata atgtaacttt cagttctcaa
    25021 attccaatgg gaacttctgt tcagcttaca gcaatgacaa tgtttggtga atacttaagt
    25081 gttcatacct atacaattgt agtaggaata gtaatggaaa actttgattt tggcttcagc
    25141 aatttcccct gggtttttga aggaggaaat tggactattt ctccagatag ttttaatggt
    25201 tctatggctg ccaaatcacc ttccattaat aacaactcca gcacttctat aagcattact
    25261 tatcaatgcc ctcaagcagg agaaatatct ttctggaaaa aggtctcttc cgaaccaagt
    25321 tgcgatttcc taaaattcta catcaatgga attttaaaat tccagtggag cggaaccgat
    25381 gatttctgga gtcaggttac ttatcctgtt tctcctggaa ctaatgtctt taaatgggaa
    25441 tatgttaaaa acaacggatc ctatcaaggt agcgattgtg catggcttga tgatattatt
    25501 ttccccagca caggtgaaca aattggaatt cctgtatttg taattgatat ggaagatata
    25561 aattttgggg aagttatagt caatacaatc gtttcccaaa ctgttactat ctgcaattgc
    25621 ggaactgctt caatgctggg aactattgca acagaatcac ccttctcttt aggagaacct
    25681 tctatgcctg cttattattt ggaatacatt attccggtag gtgaatcctt tacttttaat
    25741 gtgaatttcc agcccctaca aaactcagta tacactggaa ccctaataat aaactctgat
    25801 gaccccaatg ccttagtaaa tactattccc ttatacggaa caggacaacc ggttgccaat
    25861 gatgatcctg ttgctgtttt ggttacttct ctaaaaggat gctatcctaa tccttttaat
    25921 cctacaacta caatcagttt cagtataaaa gaaaagacac ctgtggaact gattatttac
    25981 aatattttag gacaaaaagt ccgaactttg gttaaccaac ccttagaacc aggagaacat
    26041 agcattgttt ggaacggaac tgataataag ggtcgttctg tagcaagcgg aatctatttc
    26101 tatagaatga aggcaggaaa ttattccgag accaaaaaaa tggtgctgaa aaaatgaggt
    26161 cttgaggtca agagattgta tcatacttag attgaaaaac cctttgtcat tcctgnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttctc ctcttttttc caaacgcata
    26341 agtcgtgttt ccagagaagc acttatcaat acaatataat caaagcaatc ctgtaaattt
    26401 gcctcaaaaa gaagaggaac ctcaaagcag agagcttcct cattactttg ttccactatc
    26461 ttttgaaaat cctgtaatac aagaggatgt attaaagaat ttaagtattg ggtttcctgt
    26521 tcactgctaa atactacatc tgctaaaatt ttacgattaa ttataccttt accattatct
    26581 tcattggaag gagatagaat agccgtagaa tagcgtttta ttaatgcttc tttgattgcc
    26641 gggtcttcca aatgttgatt ggcaaccaca tctgcagaaa taactttcaa gccattagca
    26701 gccagaaaat tgcaaaaagc acttttgcca ctgccgatat ttcccgttat acctataagc
    26761 agaggttttt tattttgtga catcggttaa aatttgtaga agttcttcaa aagcaacatt
    26821 ggtttgtatt tttcccgctt tggctacaga aacctgacct gtttgttcgg aaacaataat
    26881 ggcaatagca tcacttattt ctgttattcc aataccggca agatgcctag tgccaaattt
    26941 acgcacatat tctgcctttt tagatagtgg caaaactact ttagcagcca taatgcgttg
    27001 gtctcgaata ataatagctc catcatgcaa cacactttta ggattgaaaa tagtaaggat
    27061 caatcgcata gacacaatag catcaatggg ttcaccgcta taaatatatt catgcagttt
    27121 ccttttattt tccaacacaa tcagagcccc tattttacgg aaactcattg aagaaacagc
    27181 atctatcaga ggagtataaa gcgatgattt ttcctgtttg ctaaagacct tgcttagttc
    27241 ccggggcagg ttcaagcgag ctaaaataga acgcaattcc ggttgaaaca aaataacgac
    27301 agcaatgaac caataattgc gaatggcact taaaagtgaa ccaaccatct tcaaattgaa
    27361 gataacggct aaaatataga gaacaactac aaacagcaga ccccataaaa cctgatagcc
    27421 acctgattta cgcacaatca atagtgcctg gtaaatcaga aaagcaataa taaggatatc
    27481 tactatatct ttaaaacgag gaattaaaaa ttccatttag tcccttttcg ctttggcaat
    27541 agcagttaag acctcaaaaa actgcctgtg caaaagaaca tcatgaacac gcaaaatgtc
    27601 aatttcttca caagcagaaa cgaaagctgc tgcaaggcat cctcctatgc gttgtgcagg
    27661 cgacgaaggt gaaattttgt ctataaagct ctttctgcta gcacctacta caagcggtaa
    27721 gccaaattcc ttaaatgttc tcagattgcc aagaatagtt aaattatgtt ccaggttttt
    27781 gccaaatcca ataccgggat caagcattat gttttccctt aaaatccctt tggcaagaca
    27841 aaaatcaata cgctcttgaa agaaatcctt tatttcaact agcacatctt gataaacagg
    27901 atttatttgc atcgtgcgcg gagttccctg catatgcatt aaaataactt ttacctcagg
    27961 atttgttgat aaaagctctg ccaaagctgg atcggttcta agagccgaaa tatcattgat
    28021 aatagaagcg ccaagctcta ttgccttttc agcaactgtt gccttctgag tatcaactga
    28081 aaaagtaact ttaggatatt tttctattaa agagcttaga atagggatta tcctttgttc
    28141 ttgtatctct gcaggaacag gtaaagaacc aggacgagtt gattctgctc caatatcaat
    28201 aatttctgct ccttcagcaa tcattacctc cgtatgttta atagctaatt ctctgtccag
    28261 gaattgtgct ccgtccgaaa aagaatcctc agtaacattc aaaataccca ttaaaacagg
    28321 tatccgttcc ggaaagcaat atttattcat taataacaaa cctgcaacgc aaacgataaa
    28381 gttgtttggg gttataagtt cccttaaaac caaacttcca ggaaggcatc acttttctca
    28441 ctgcttcagc ataagttgcg ctggtataag aaagtatatt tacgctgctc atatctactg
    28501 tgcctttagg agtaagttta aattccagat agacctcacc ctcttcagta ggatttattt
    28561 gaggtttggt ttggctgatt atatatgctt ctcccccacc ttcttccagg gaagaagaaa
    28621 agccgaaatt actgcctgtt atggttccgg gtacttcttt caaggcattg gaatttctat
    28681 tatgaggaaa gctggaagga attgtcgttt ttgcattatc ctgaggtaac acatttatcg
    28741 gcatagcaat agcaggttca ctatcaccga tacttacagg atttccatta acggtattta
    28801 tttctttttt ttcggaaatt cctgcttgtt tagttattcc ctcagttccg ggaatattgt
    28861 tacccatagt tatttcttcc agtttattag ctgtatgcgt ttcaatcatg gtagtcctat
    28921 ctaagggttc ccattcaaaa gtataccatt tttccggaac ataaggttgg ataacataga
    28981 aggcaaaaag aataaacaac aacaggtgaa atgctacaga gatgaaatag ccgttgaaaa
    29041 gtttggaaaa ttgaaaacgg cgcactattt tttctccaaa acggttgtgg caacaaagat
    29101 tctttcatag ccggaattac ggacaatatc cattaaattt atcagttttt gcaccggaac
    29161 ttgtttttca acagcaatgc gcacaacttg ctcaggattt tggtattttt gctgaaggac
    29221 tgtttccagt tctttaattt ctatgggctt atccataaaa tacaggcgtc cgtcattata
    29281 atatgtaatt aacagagttt gataactttg ccggaccgca gtggaagaac caggaagctt
    29341 aatcggtaat ccggtttggc ttacgaagtt tgaagcaatt agtaaaaaca gcagaagaag
    29401 gaagataaca tcggtcattg aaataagcat tgttgtagaa attcgctgtt tggaaacacg
    29461 cattttcata gtttaccttg tgttaacttc ttatatttaa tgatatattc cactgcattt
    29521 tcctgcatat ctttcacgat attttccaat ttttgcacca gatcgttgta aaaaatgatt
    29581 gtcgcaatac ctacaattaa accccctacc gtagttaata aagcaaccca gattccacct
    29641 gccagcatag aaatatcaac tccgctttga ctggctgcct gaattttcat aaaaacattc
    29701 accatcccta taacagtgcc caaaaatcca atcaaaggcg caattgccgc aaaagtagaa
    29761 agccaaccca gtcctttttc caaacgatgc aattccaaat ttgccgttgc ttccatactc
    29821 tggttaatta aatccaagtc gtcgctttcg ttgttgaata gtttatccag catcagtccc
    29881 ataggacatt cacttttatg catatttata agagcgcgga tattttccag tttatactgc
    29941 gatttaagag acagctgaag tttattgtta gatagacgta cttttctgaa ttgacggtat
    30001 ttttcaatta tgatgccgat gctgattacg gaaataaccg taagcacata catcaggaat
    30061 ccacctttca cgattaaatc taagatagac atttaacctc gcgtattatt ttttctctta
    30121 taaccttaac tgctgtagca tccataagga tactacagca gtttttaata gtcagggtct
    30181 tttcaagtat gaattatcca ttctgttttt ggaaattttt cataaattca gccaaatcgt
    30241 aagcagcatt aagaggaagc gcattataac aggaagcacg gcatccacct acatcacgat
    30301 gacctttcag accaatcatt ccattctttt tagcttcgct aataaaaaga ttttccagtt
    30361 cttcgctggg aaggcggaaa gtgatattca tcaaagaacg gtcttccggc accacagttc
    30421 ctctatagaa accattggag gcatcaataa catcgtaaat gtattttgcc ttagctttgt
    30481 tattttcttc aattttggca agtccaccat agttctcaat ccattttaga accaaaccaa
    30541 tcatatagat agtgaaacag ggaggggtat tatacaaact gccgttttta gcatgtaact
    30601 gatagtccag cattgatggc agatttgtag ctcctgtagc caaaaagtcc ttcttcacca
    30661 aaataacagc acagccggca ggaccaatat ttttctgtgc tccggcataa ataaagctgt
    30721 atttgttgat atcaataggt ttgctcataa agtttgagct catatctgca atcaaaggaa
    30781 catctttcgg aacatcaggg tctattttcc attcagtgcc gtaaatcgta ttgtttgtag
    30841 taatgtgaag ataggcagga ttatcggaaa gttgataggt tttcggaatg tagctgaaac
    30901 ttttatcttc cgaactggca gcaatatgca ctggtttacc caatttttta acttccgcca
    30961 ttgctttttt gctccacaca ccggtatgaa tgaaattcgc aattttacct tccggcaaaa
    31021 agttcatagg aaccatcacg aattgcaaac ttgcacctcc atgcagaaaa agcacatccc
    31081 agtcatcacc aagaccataa atccgttttg cggcatctct ggcttcattg ataatggctt
    31141 caaactcttt actgcgatgg ctcatttcca taacggaaag tcccatccct ttgtaattaa
    31201 caagatctgc ctgtgcttct ctaagcactt cttcaggcag aactgcagga ccagcgttaa
    31261 agttatgtac gcgttccatt tgtatcctcc tggatatata ttttcaattt aaaacaagtt
    31321 tttctaatac caccctttta ggcaagacat ttttttgtaa atagagaaat gtattgactt
    31381 aaaaccgctt ttttattctc tgtgacaaag gtttaatatg atgatacacg aattgttacg
    31441 ccataaaaaa gtaatccttg cctctgtttc accaaggaga aaggaacttt tttcgctttt
    31501 gggaatttcc tttgaagcca ttcccgcaaa aattgaagaa cccattaata atgaagcacc
    31561 gcaaactcag gcaatgcaaa atgctctacg aaaagctcaa cttgttaagg ataaagtaga
    31621 taaagatgcc ttagttgttt ctgccgatac tatagtagtt ttggataatc acattttagg
    31681 caagcccgca gatactgaag aagcaggaaa ctatttaaga ttactttcgg gacgcacaca
    31741 tagtgttttt accggcatct gtatttatta taatgaaatt gctaacatta attatgagca
    31801 gacatttgtg acttttgcag agctgtcgga aaccgaaatt gattcttatt tggcaacggg
    31861 agaaccttta gataaagcag gagcttatgg aattcagggt tttggagcac aatttgtaac
    31921 aaaagtggaa ggatgctatt tcaatgttat gggctttcct atccgcttat tttatgaaat
    31981 gctgaaaaca atcttagttg aggaagaaca gtgaaaatat tagctaatct ctatctctgg
    32041 ctgaatggta aatttacaga agaaacccaa tctttattag tagacaaggg aataattatt
    32101 gaacatctac cctatcctgt tccggaacag tataaaaata tttttcaaca ggattttaac
    32161 ggtgcttatg cttatcccgg ttttattgat actcacactc acagctttga aggtggttta
    32221 tattgctcag gtgtagattt atccaaagtt aaaaacattg ctgaggttct ggaattgctt
    32281 tctgctgaaa atgccaaaaa gacaaaagag gaaattattt ttgcctggca actggatgaa
    32341 accttattgc aggaaaagcg tttccccaca gttcaagaac tggattcggc tgttcccgat
    32401 aaagcattac tcttaagaag aattgatggt cattcttgta tgctcaattc ttttgcccgt
    32461 aaacagatta aagaattaaa tacaaaaaat gaagttttaa agggagcaga aaacgatcgg
    32521 gcagtccgcc attttcatag ttgcttaacg gatgaggaga ttatagaagc ttatcaggta
    32581 tccgcaaaaa ttggtttgca gggtggcttt acttctttgc atacgatgat tggtgacgct
    32641 cataacagca ttgctcacta tgagcttatc agagataatc tggataaatt ccctctgcgt
    32701 tttatcctct atcctcaatc cttcaatatt agtgcagctt tggatgcagg agccaaaaga
    32761 atcggaggat gtattttagc agatgg
//
LOCUS       DAOOZV010000014        32758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1505_length_32758_cov_57.146684, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000014 DAOOZV010000000
VERSION     DAOOZV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 32758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32758
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_32758_cov_57.146684"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1245
                     /gene="hydF"
                     /locus_tag="PLW08_02710"
     CDS             25..1245
                     /gene="hydF"
                     /locus_tag="PLW08_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPL51545.1"
                     /translation="MSSTPRGERLNIAIIGKRNAGKSSLINALVGQDIAIVSAVPGTT
                     TDPVDKRYELLPLGPVTFFDTAGVDDTGELGEKRIKATQKILYRTDIAIFVSDGNAFN
                     NAELKMIEKIRDMELPMLIVFNKKDIIPASQENIAFCQKYSLQYISVSSVTKEGIDEC
                     KEKLIQLSPKYLKENRIIAGDLVEPKNSVILVTPIDTSAPKGRLILPQVQTLRDLLDR
                     NCLVTVVQETELKSALDKTKEPPSLVITDSQVIEMVANIVPEEIPLTTFSILFARYKG
                     ELDILLEGINRLKTLQDGDKILIAEACSHHIQKDDIGRVKIPNWMQKFSQKNLNFEVY
                     AGHDFPENLEEYAVCIHCGGCMITPMEMNRKIMECRRRGVPVTNYGMTISLLKGVLDR
                     TIKPLLKAKVKQTQ"
     gene            1242..2033
                     /locus_tag="PLW08_02715"
     CDS             1242..2033
                     /locus_tag="PLW08_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPL51546.1"
                     /translation="MKISALQFAPRLLDKEYNLKKMKQLLSVLKTDLVVLPELCNSGY
                     VFANKEEVFSVAEEAGKGETYFALSEIASCNNCNIVYGYPELDGNNLYNSCMLIMPDG
                     RFYNYRKTHLFNREKLFFSPGDTGFQVFTILNGVKIGMMICFDWQFPEAARTLALLGA
                     QIICHPANLVLPWCQQAMKTRCLENRVFAVTANRIGTEINAGETVSFTGQSQILNTRG
                     EVLSSLSVSEERTITVEINPEEANDKTVTERNDAFKDRRPEFYKL"
     gene            2030..4033
                     /gene="ligA"
                     /locus_tag="PLW08_02720"
     CDS             2030..4033
                     /gene="ligA"
                     /locus_tag="PLW08_02720"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPL51547.1"
                     /translation="MNKNELKARIDRLRAEIEHHNRLYYTLAKPEISDYEYDMLVKEL
                     QELEAQYAEGAIEESPTQKVGSDLTPGAKTIPHKVRMASLDNAYSLEEVLAFINRTNN
                     DLGYASEYCAELKIDGFSINLYYENGNFIYATTRGDGLEGEDVTENFRLLPDVPFTID
                     YKGAIEIRGEIYIPVQDFLALNEFRRENELKTFANPRNAAAGSIKLKNREEVKSRHLK
                     ALFYALGYYETPLQYKNEIIDKQTRLLDFLDELGFPTSKRTLCKNYQEVEKFCLQMEQ
                     ERYTIPYDIDGIVIKVNSLEDQKKLGWTAKSPKWAIAYKFKPEEKETILESVSFQVGR
                     TGAVTPVANLKPVFISGSTVSHATLHNADEIKRLDLHYGDTVLVVKSGEIIPKILSVN
                     VSKRPVNAKPVIFPATCPVCNAPLQRDEDGSINYCPNANCPAQIQRKLEHFASREAMD
                     IYGLGSSLIARLIETGQIKWIPDIYHLDYNKIAEMERMGKKSADNLQKAIEASKTKNF
                     DRVLFALGIRHIGLITARTLAQHFKNIDALIMAKEEELLSVPDIGSIIARSLISFFSQ
                     KSNRELIDSLRKVGLQFTYISPQSSAILAGKTFLITGTLPNYSRKDMETLITEHGGKI
                     LSGVSKQLNYLIVGENPGSKLDKARQLGTVKIISEAELLDMIK"
     gene            4030..5358
                     /locus_tag="PLW08_02725"
     CDS             4030..5358
                     /locus_tag="PLW08_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPL51548.1"
                     /translation="MKILKRYILKEHIAPFFIALMVVTFVLLSDRIIDLMNLIIEKKL
                     PWQTVFEVFGLSLPYMLALSIPMAVLVATILAFGRMSVDREITAIKSSGINVYSLIGP
                     LVLAALLLTGLMVYFNHWFLPDTNHKLKNLMLKIAYYKPMTIIKEKEFTNFMDYTIYA
                     RETTDSLLTDVLIYDRSQSHFPRTIFAKSGNVIQKDNGNSLQIILNEGEMHERNEKEV
                     GKYQKTTFSRYIINVRNLGSNMEMFETGYRSDREMTYNQLITAIGEHKKELQTKQQEI
                     EELNKRIELLQKSPQNFTRNMELRRLQAMQKMVIDRTQELKELINSLLVEYHKKFSMS
                     FAIIIFVLIGIPLGLMTRTSGIGMSFSVSSIIFLVYYIALNGGEQLADKGFMPPFLAM
                     WLANIIFLILAVLLIIGSIKEKRLLDMPTLIWKIKHYRNRKAPVPDEIVH"
     gene            5361..6449
                     /gene="lptG"
                     /locus_tag="PLW08_02730"
     CDS             5361..6449
                     /gene="lptG"
                     /locus_tag="PLW08_02730"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HPL51549.1"
                     /translation="MRILDKYIIREFLRTYLIIFLSFVAIFVVIDVIDNLPRLIRNGA
                     TAQQATIYYLMRLPYLLVLTSPVTVLLAGLFMMSALSKHNESIAIRAAGISIKRAMLP
                     LFGIGLIISIVVAVFGEYVVPWAETTKNYVYNVQIKGQQPDDQMLKARIHYQGKANDF
                     YYFGFFDGYKNNLKIIDLTRIDFKNKQITEHITAASADWNGSRWILKDCDIRRFANGK
                     QVFMAHYPQTDLVILDVQPQDFIRISQKTLSLNFWQLKEYISRMKKLGDNPAREIVDL
                     NMKISFPLTNLIVIFFFIPIATSNIRSKGRGWIIMLGLVVCFIYLIVVRVCQSLGYNS
                     VINPFWAAWFPNIFFTLLGLGFLYKAEI"
     gene            6808..8886
                     /locus_tag="PLW08_02735"
     CDS             6808..8886
                     /locus_tag="PLW08_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL51550.1"
                     /translation="MADNNRIAIHPILPIPEREEVFFYWNGQKLKAKSGEMIASALIA
                     NGISIFGHHHKDGSAQGIFCANGQCSQCSVIANGVAVKSCMTAVSENMIVQSLEGLPI
                     LPAEDTPLNFEPLEYIKTDVLIIGGGPAGLSAAIELGKQNIQTLLIDDKNALGGKLVL
                     QTHKFFGSQEDSSAGTRGIDISKNLSEELAKFGSVEIWLNSTAVFVFSDKKVGILKDG
                     VYKIVEPKRILNTAGAREKFLRFPGNNLARIYGAGAFQTLVNRDLVRPTNRLFIIGGG
                     NVGLIAGYHSLQAGIEVVGLAEAMPVCGGYKVHSDKLKRFGVPIYTSHSILCANGKES
                     VESITIAEIDKNFQPIPKTEKTFACDTILIAVGLNPLNEFTWEAMDAGIPVEAAGDAL
                     EIAEASSAMFNGKIAGVKIAAELKGNKDNPIPKEWYAKAEELKSPPGITKSYQTPEKE
                     EGVFPVLHCLQEIPCNPCTTVCPTNSIKTEDGSLMAVPVYQGSCIGCGKCLLICPGLA
                     ITLVDYRKDKENPTVSIVYEVTNYPVQIGDKKILNDIDANELGEYAITAVQDFPKQHT
                     QIVKFQVPKAIAKKVAGFRIQDASVSAPIDKPIIFEKTSDEAMICLCERVSVGQVRAL
                     IKKGITDLNLIKAITRAGMGPCGSKTCEVLIKGLLREEGIPEKEVVANTKRPLFIEIP
                     LAKFPDGSVK"
     gene            8883..10037
                     /locus_tag="PLW08_02740"
     CDS             8883..10037
                     /locus_tag="PLW08_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPL51551.1"
                     /translation="MKNIYDIIVIGAGSIGLPTAYNLAKAGMKVLVLEAEHSPGQGNN
                     KKAIGGVRATHSDFGKINVCLRSIEILKNWQEETGEDIGWLSNGYSYPAYNEKDAQEL
                     QDLMKIQHSFGLNIKWLSPDEYSEIVPGILQEGLQGSTYSPEDGSCSPLLVSAAYFEH
                     SQKAGVEFHFQERVTGFDIRAGKIYKVITNKGIYSCAKVVNAAGSYAREIGKLANLDL
                     PVFPDNHEAGITEPVERFFGPMVIDMRLAPGSANFYFYQNIEGQIVFCITPEPPIEGI
                     DNRSTSVFLPMCSKRMLTIYPRLRNLKVRRTWRGQYPMTPDGFPLVGIMQEVENLVNA
                     VGMCGQGFMLGPGMGELVTRICLESATDKDLKILESFDPYRQFTSQEAFK"
     gene            complement(10185..10832)
                     /locus_tag="PLW08_02745"
     CDS             complement(10185..10832)
                     /locus_tag="PLW08_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPL51552.1"
                     /translation="MKPFLLFDFDGTIADSLQLGLKIANNLAPQFGYPPFTENDIQHF
                     RSLTWHKIAREMQIPFYKIPKIVTIAFKEYKRLISELEPCAGIVEMINELALKEIPMA
                     LLSSNTVENVHLFLTNHKIDSFLWIEGTSGILNKAKDIKKRLKKHKISTNEVIYIGDE
                     IRDIEAAHNCGLKVIAVTWGFHTAEFLASYKPDYLVNEPKEIVEIVNRLTVKSEK"
     gene            complement(10829..11692)
                     /gene="rsmI"
                     /locus_tag="PLW08_02750"
     CDS             complement(10829..11692)
                     /gene="rsmI"
                     /locus_tag="PLW08_02750"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPL51553.1"
                     /translation="MPSGNIYLIPVPIGNLGDITLRALELLKKAELIACEDTRVTAFL
                     LQQYKIPVPKLISFHKFNEKQREEYFFEYLQTGKDLMIVSDAGSPAISDPAEHIVKEA
                     IAKGIKVFALPGASAILPALSVSGFSTRQFQFVGFLPQQPISRRENLEQIAEYPYTTV
                     IYEAPHRIWKLLNELYAVCGNRKICICREISKLHEEHIHTTLEEINKNPSLTIKGEFC
                     LILDGQKLKGEEEKEQEELNEDDLINYINSALDLGLKTKDIRDMVCLKSSLSLKDAYQ
                     LVISVKKERGK"
     gene            complement(11751..12941)
                     /gene="kbl"
                     /locus_tag="PLW08_02755"
     CDS             complement(11751..12941)
                     /gene="kbl"
                     /locus_tag="PLW08_02755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPL51554.1"
                     /translation="MAYGKIKSDLQELFKELKEQGLYKEERILTTPQGARIGVQGGKE
                     ALNFCANNYLGLGNNPEVIKAAQDIMNDWGYGCNSVRFICGTQAIHKQLEEAMSKFLG
                     MEDTILYAACFDANGGLFEPLLGEDSAIISDELNHASIIDGVRLCKAKRLRYKHSNME
                     ELENALKESQDCTYRIICTDGVFSMDGDMAKLDEICDLADKYDSLVMVDDSHATGYIG
                     KNGRGTPEYFGVTDRIDIITTTFGKALGGANGGCTSGRKEIIELLRQRSRPYLFSNTL
                     APSIVGATLKVLEMLSHSNELRDKVWENASYFRKEMVSAGFDIVPGNTAIVPIMLYEA
                     PLAIKMADMLLEEGVYVIGFVYPVVPKGKARIRVQLSAAHTKEDLDFTISAFKKIGRE
                     LGLI"
     gene            13130..13846
                     /locus_tag="PLW08_02760"
     CDS             13130..13846
                     /locus_tag="PLW08_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51555.1"
                     /translation="MQKIIIILCTLVCLSFGAEAISIYEIQFTASAGSDGTFPSPYLG
                     KTVSTEGIVTAMDFKEGGYFISEKLNGPWRGILILDRNPKVKKGDFIRVSGIVSEFYG
                     MTCLQDISQTKVISSNNPLPQPVLLTTGQLSNAGEAEAYESVYVRFLNVTSAGNKSLK
                     NRFTVTDGSGTCLVLNNQFGIERNNKIALNAQYSSLTGIVIYGYSEFALCPISTSDYV
                     IQQPMSVQNRSWGRIKSIYR"
     gene            13872..14240
                     /locus_tag="PLW08_02765"
     CDS             13872..14240
                     /locus_tag="PLW08_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51556.1"
                     /translation="MKKFFKKILILGGTLAAAHFGFKAYKKISGTAKLSKSLPEFLNN
                     VYGEMPQVNVNHTLNSTRIKVFFSQEILDKHTDIETTIREYIDDFYPEIAKTSVDISI
                     MPATEETEDIPVEEETEEKE"
     gene            14781..15197
                     /locus_tag="PLW08_02770"
     CDS             14781..15197
                     /locus_tag="PLW08_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL51557.1"
                     /translation="MNKNSNERMQVRYSDAFKLKVVQDIEKGKLTISEARRLYEINGT
                     HTVYRWLRKYGKNDLIQKIVRIEMKDEKSQLKKKDERIRELESVIASLTLHNLCLQNY
                     VDVVNENISEEEKKTSLSKLSPEQKQVLMRLLSKAR"
     gene            15194..15994
                     /locus_tag="PLW08_02775"
     CDS             15194..15994
                     /locus_tag="PLW08_02775"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPL51558.1"
                     /translation="MIKICSNLGVSKSWYYETMANREKVRQYEQQIVNEVQEIRKEHP
                     VYGTRKIWHQLARDGTIIGRDKLHKILLKYNLTQPKHRKKVRTTFPGMYEQGFINLIK
                     NLTIDHKDQVWCTDITYIYTTEGILYVSGIMDIFSRKIISFNVSNNLRTEGSLVCLQK
                     ALKQVANPQGIIHHSDHGVQYCSGRYLEALLSKGMQVSFTGPNHCFDNAKMERFFNTL
                     KYEYGLNAVIKSKKLAIQMIKNAVQNYNYTRLHAAINYMIPCELYDVA"
     gene            16074..16451
                     /locus_tag="PLW08_02780"
     CDS             16074..16451
                     /locus_tag="PLW08_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51559.1"
                     /translation="MEKQIPAKQKQALLGSNLPGITGTNKGVNKSERNDLVMLTPVWG
                     MHKKIPNQYPLRTLLNLKNSELHKPINGALYPIFLSYIGLILLTYNNQDRLQNIMCHL
                     SQNLKMKKPKKQSAILGLDMGHF"
     gene            complement(16651..18486)
                     /gene="glmS"
                     /locus_tag="PLW08_02785"
     CDS             complement(16651..18486)
                     /gene="glmS"
                     /locus_tag="PLW08_02785"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPL51560.1"
                     /translation="MCGIVGYIGHRNALPIVVEALKRLEYRGYDSSGCALIHDGELQV
                     YKRAGKIIELERCLPEPNKCTGNLAIAHTRWATHGEPNEVNAHPHLDCKGEIAIVHNG
                     IIENYKLLRDKLLELGHTFISETDTEVIVHLIEQFLLSEPTLEDAVRSAMTKVEGTYG
                     LVVMSLKEPDKLIAVRKGSPLIIGIGENEHFITSDVNAIIIHTKRVIYLQDDELCVVK
                     ADGFEITTLDKRSVKPEISVVDWDISAIEKGDFKHFMLKEIFEQPVTIENAFRGRINQ
                     HLGTARLGGLGIPNFELRKIKQIHLVACGTSFHAALIGKYIIEDIARIPVFAEYASEY
                     RYRNPIIPEDTLVFVISQSGETADTLGALREAKAKGARVLAITNVVGSTIARESDGGT
                     YIHAGSEIGVASTKAFTSQVTIMTLLAILLGRMNYLSTVKGMEYISELENIPSKVEKI
                     LALNEYIRNIADTIKDYKNALYLGRGINYPVAMEGALKLKEISYIHSEGYPAAEMKHG
                     PIALIDENMPVIAIATKDPLYDKIYSNLQEVRARKGRLITIATEGDTELSKISENVIY
                     IPDTLTNLQPLLTVIPLQLLAYNVADLKGLNVDQPRNLAKSVTVE"
     gene            complement(18474..20486)
                     /locus_tag="PLW08_02790"
     CDS             complement(18474..20486)
                     /locus_tag="PLW08_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HPL51561.1"
                     /translation="MNFKHSLVIYRKELMEVLRDKRTIFTTFILPIILYPLIIVGFNS
                     IMIRQTKSLEERGATVAVQDSVNNSISRQLIQDLTKIKNYTIIPYSETTSRLYEDKDI
                     QCIVTIRDSLGSDGTQFFKVYIQYDKSKEQSRMVFNKLSEQLSKTEKELQKELLQSFG
                     ISPDFLNLIDIRERDTSSAQKKMGMLLGMFLPYIVIMMLFAGASIVAADLVAGEKERK
                     TLETLLVSSVGRLEIVCGKYLTIITLAMLNMIINLFSISFSLKFMLANQSTEMAGVSL
                     PLNAILILLIAMIPLATLFAAILLSISTFSRNIKEARSYEQPLLIIAMLLGMVSFFPA
                     IEINNLMALIPIVNIALLFKGVLINEFTISQILLTIFSTVVLDVIAIIITIKLFNTES
                     VLFRTEEESGGLKVFKRKPKTFFNPYNGILYFSIALIVLYYLGSYWQTKDLFSGLIQT
                     EIIIIALPVLLILRFLRLKPKEVLRLKNPNWNSFLLIPFIAVSAQIIVSIISQLINIV
                     FPFPEKYLEALSQLYKMNETPWKVFMAIALLPGICEELLFRGFIIRFFEKYSVRWAVV
                     ISAILFAAYHLDPFRFVPVLLLGLLLGYLAIRSGSIYTSMFSHIIINGMAFVLVTYSN
                     TGWVKFIAKDGENLNYWLIVPALIIFCLSLYAFHKVTAKGEDKCVE"
     gene            complement(20476..21243)
                     /locus_tag="PLW08_02795"
     CDS             complement(20476..21243)
                     /locus_tag="PLW08_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL51562.1"
                     /translation="MIKVENLSKVFTKKDGTNFYAVDKINFTAGDGEIVCLLGVNGAG
                     KTTTMRVLSTIFQPSEGTAEIQGWDIKKHPNKVRENIGFLSGDTGLYDRLSGREFITY
                     FGRLYSIADNIIKQRISEMATLLDMNEFLDKKIEFLSSGMKQKVSIVRSIIHYPPVMI
                     FDEPTAGLDILTSRNIISFMRDCKKRGKCVLFSTHIMREAERLADRIVLIHKGKILAD
                     GTLEDLRNTSSQTDLDDIFVYYINQYTDETELRANEF"
     gene            21516..22412
                     /locus_tag="PLW08_02800"
     CDS             21516..22412
                     /locus_tag="PLW08_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL51563.1"
                     /translation="MKEKATLILGGGSALGLAHIGVLSVLEEQYEITGIIGSSMGSII
                     GGLYSSGLNSEEIYKIAKSFKNTRIFSPLNLDRTIQGIFDGKSLLKAFLKWTEGKKIE
                     EGRIPFIACAYDLISKSTVLFNKGLYADAMRASTSLPVLFAPYKFKQYLFIDGGIEHP
                     LPLAFSSVLSQDKVIAVNVLPEAQKSACFINLESDEITKSKRLGRTEVIFNAVLQNQA
                     YLAIRDIAAFSPDIVIEAGMADGHPFAFHKARDFYNYGRKQTIETLANYKEPRFITAI
                     RKHYRNLIRYLPNLNIPVKDNT"
     gene            22545..23282
                     /locus_tag="PLW08_02805"
     CDS             22545..23282
                     /locus_tag="PLW08_02805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL51564.1"
                     /translation="MNEIIKILSKVDGGNVLDAATGKGDFITTLKQHLKSYTQIIGVD
                     SSQRCVDYAQKIFPENNIEIFRMNLEDMQFEDNYFDMVCMSDALHHIKNREKVLNEMI
                     RVLKPEGLMLITEMYCDGKQTPAQETHIIMHHWVASVDRLLGTYHQKTFTRKELQDII
                     KKMPLKKVQITDFYVPVDDPSKNCPTLLRNCQEIIKRLQTMENTEALIKEGQELMQRI
                     KDIGYASACRLAITGYKKSNYNAITNS"
     gene            23298..24557
                     /gene="rho"
                     /locus_tag="PLW08_02810"
     CDS             23298..24557
                     /gene="rho"
                     /locus_tag="PLW08_02810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPL51565.1"
                     /translation="MLFEEDLFSLNQLQLIRLAKKIGLPGYTQYKGTELIFKMLEFQA
                     AQEGLAFVTGCLEIMEDGFGFLRFPQNNYTAGRDDVYVSLTQIKRFGLKTGNMVSGPV
                     RSPKEGEKYYALLRVESVNNMAPDCMQNLRPFDELTPYYPTERLNLEFDPDNYSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQDTANAILSNHPEVYLIVLLVDERPEEVTEMK
                     KILKPGSREVISSTFDESPKNHSAVSEMVLEKAKRMVELHQDVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGSARKTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRTISDMRLYPAIDLVKSGTRREELLLTQNELNRMYVLRKYLKTIS
                     PIQGIETLRKKMQATQTNEELLNSMSN"
     gene            24557..25729
                     /locus_tag="PLW08_02815"
     CDS             24557..25729
                     /locus_tag="PLW08_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPL51566.1"
                     /translation="MLKSPFPAVKQVFLFILLTGLCCFAFAAVRKEFQDLDKLYNSGK
                     LDELSANISTLKPVNDEERACLGFYNALLKVKIEDAISAHQWLIEKFPKSPYAQKSLL
                     ELGKIFILDRKIEEATLYLRRITSPEIIERFYWLGLCAWWNDDYANAISNCENYLRLA
                     PRGEFAEKSYYLIAECYLEQKKAYSAVATLLKLQNAKLPEMDEQYFYYRLGYAYELSD
                     KPIDAITAYRKGYEQDPYSQVAYQIEDRILELKSSKPNIDISFLYPYSPLQVTIVQEE
                     QPDTLKTSVTTDSKSSSSSTAKQIEVKQDTPVKLKEKPKEGYWLQAGRFSVESNANRL
                     VINIRLLNIPAAYYEDVSGGKKSWVVVCGSFPDRAKAEEAKELLATNNINSFITAY"
     gene            25732..28362
                     /gene="mutS"
                     /locus_tag="PLW08_02820"
     CDS             25732..28362
                     /gene="mutS"
                     /locus_tag="PLW08_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPL51567.1"
                     /translation="MDDNKLTPMLKQYYAVKEKHPDKLILFRMGDFYETFFEDAHTAS
                     RILNITLTTRNKNDENPVPLAGFPYHALNTYLDKLIKAGLKIAICEQTEDPKKAIGLV
                     KREVTEIITPGAVLDQSFLEGNANVFLASLYYNDPQKKIGLAHLDISTGDFLFTELDK
                     EELVNELQRFRAAELIVDSTQAEEFVKTLPLETLPTITVFDSWQFQPQEATATLKKHF
                     GVTTLEPYGAHNKLLGATAAGAALAYVQGLYNTPFNHISSLRYYSLSQYMQLDEISRR
                     NLELVRSIRYGTKYGSLLSVIDQTMTPMGSRLLQQWLLHPLLDIKEITFRQDIIQSFI
                     DKSSHLKELRLILKEIGDITRLVTRLGSLRINPRELIALKSYLYSARNLQTKLSTFEH
                     PQFAVWQHNMGSFEDIIALIEKAINDNPPVSITEGGIFAKGYNPELDELMDIIYDGKS
                     WIARLEEDERRKTGINNLKVGYNRVFGYYIEVSSANKNKVPDYYIPKQTLTNSERFIS
                     PRLKEFEAKVLSSEEKIKNLEYELFKELRQNLAESLPRFQQLSEVIAELDVLSSLAFL
                     AWQNQYSRPVFTESRELHIIDGRHPVIEKLMESDKFIPNDTHLDYPETSIAIITGPNM
                     AGKSTYLRQVGLLVILAQMGSFVPASKMTLPVFDRVFTRVGASDNLAQGQSTFLVEMI
                     ETANILHSATSNSLILLDEIGRGTSTFDGLSLAWAIIEYIQKYKHSLTLFATHYHELT
                     ELENLYPDIKNYNVAVKQWNEEMIFIRKIERGGADQSYGIQVARLAGIPEKVIRRAKE
                     ILKNLEEHEISPQGLTATIRKKLVRDVPQIDIFEILADKASENDPIINEIKEIDLNKL
                     SPIEAFQYLQKIQNQLLGEK"
     gene            28359..30056
                     /locus_tag="PLW08_02825"
     CDS             28359..30056
                     /locus_tag="PLW08_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL51568.1"
                     /translation="MNKLPTFIVITLLGLFFLVSCATNSSSVAGSVNGTEISLEDFFN
                     SYRGHYAIFGYQTGRTPTKEEKEKLHNETWRDITKAIILQDYYKKYNISASVAEVLDT
                     LSNSIPEHIMKSPRFQVNGKFDKKLYLQSLMTDQPENLAALRKHYQENVIPILKLQKV
                     LIEEEMLSSKIRKQVEQIIASNADLELYIFAPSQLEISLSDAEISAWYQTNLEQFRLW
                     QFYRLGYCSIPVIPDSTELSDAKNTAEFIRNELARGIEIKDIIANLENKNSLVSFIDN
                     GYQKTEELPWEVQNALSGLENGRCSEPLPYEKGWIMYQKIQSTKTLTLYNTIFVQALP
                     KTASLLAPETQAQQIMKLALNIGLQQAADEFGISYTLTPAMSPDSLIVPANDIKGKLM
                     KYLEIANSGDIIEPLYSAELSAWLIFEVVEKQSKEYLPLEEVKPQIVQILSKERRKDQ
                     NKQIVERWLINQNIPANYQWEKLEQVTIDSLWQGKPLTRIYYQAVKAYLEKANPPQIT
                     EGEVIIVPKVTAFRPQKTKISPERIKTIYTQNLPEDWFNSWMEEQVKKAKVVINTKP"
     gene            30053..30520
                     /locus_tag="PLW08_02830"
     CDS             30053..30520
                     /locus_tag="PLW08_02830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPL51569.1"
                     /translation="MTCFSSQNHYLFMQEAIAEAKKAFAEDEIPVGALLVKNNTIILK
                     EHNRSQQLANPLAHCEKLLIDKILTSEPGFLYDYTLYVTLEPCLMCAGMIILSKIGTV
                     VYGAKDPKTGVVGSLYNVLNDKSFNHHPVVIGGILEQECSFLLEEFFRKKRTL"
     gene            30529..30621
                     /locus_tag="PLW08_02835"
     tRNA            30529..30621
                     /locus_tag="PLW08_02835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30563..30565,aa:Ser,seq:cga)
     gene            30642..30986
                     /locus_tag="PLW08_02840"
     CDS             30642..30986
                     /locus_tag="PLW08_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51570.1"
                     /translation="MIHKDYLWADANIPLISCDRLRDELLIYNLDESATANLIDQFEK
                     LTGKTIDKFYKIEELSGGQKVILMALLAIYSPAPKIRFVNLLNALDPKRRDAIQSLIR
                     NSGKDIILDESG"
     gene            30983..31507
                     /locus_tag="PLW08_02845"
     CDS             30983..31507
                     /locus_tag="PLW08_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51571.1"
                     /translation="MIKIAPGIVSCWQDFSLLIEQELFFLPENIYYLQGENGSGKSSF
                     IKHSLLPVLETQRNLFYFLYFQQLFHLQGYAIKSHSAFYQPELKLKSEWDCIQYLLHN
                     LSEIYAIESKPVYCIVDENRYLAEVYHYLKESSIPFCLIFCEHSSFSIAEEVNIINFQ
                     LLTPNQSRVYETTI"
     gene            31491..32102
                     /locus_tag="PLW08_02850"
     CDS             31491..32102
                     /locus_tag="PLW08_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL51572.1"
                     /translation="MKPQYRLLLVSLALIVFFVFFCLIYLENIPVQLVVLGVVLLLSA
                     WTFKLKGLLKKLYHFLPFILLLFGVYFIFALFQIGQNKDYWIHYGITRTTLLISSLMF
                     IQVLITWLKIDTFLDFPLGIEKLKYIILGKMLYKIAFSSYSELCLFVDSIPAEQASKI
                     TLKKKFRKRLIVLLALITYVINEATLKGEMIDERIWHCHQVPK"
     gene            32137..32604
                     /locus_tag="PLW08_02855"
     CDS             32137..32604
                     /locus_tag="PLW08_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPL51573.1"
                     /translation="MKWYLIVGLTVMVMIATVLVRIPVPGGGYFNLGDVVIIFSGLFG
                     GWKAGLIAGGIGSALADLIGFPVFAPITLIVKGLEGLVAGLAKPAIKWQIYFFPAIAV
                     IIMIAGYFLGCWLFPNLGLAVAIADLPVNIIQAVAGYIGGIALHLAYTKISST"
BASE COUNT        10515 a         6013 c         6293 g         9937 t
ORIGIN      
        1 aaagatttta tattggagaa atagatgagt agcacccctc gtggtgaacg cttgaatatt
       61 gctataatcg gaaaaagaaa tgccggtaaa agcagtttga taaatgctct ggtggggcag
      121 gatattgcca ttgtatcagc tgttccaggc acaacaacag accctgttga taagcgttat
      181 gagctcttgc ctttgggtcc cgtaactttt tttgacactg ccggagttga tgatactggt
      241 gaattaggcg aaaaaaggat taaggcaacc caaaagatac tctatcgtac agatatcgct
      301 attttcgttt ctgacggtaa tgccttcaat aatgccgaac tgaaaatgat agaaaagatc
      361 agggatatgg aactgccaat gcttattgta tttaacaaaa aagatataat accggcaagc
      421 caggaaaata ttgccttctg ccaaaaatat tctctgcagt atattagcgt ttcttccgtt
      481 accaaagaag gtatagatga atgtaaagag aagcttattc agctatctcc caaatatttg
      541 aaagaaaaca gaattattgc cggagacctt gtagaaccta aaaacagtgt tattttggtt
      601 actccaattg atacttctgc tcctaaagga cgccttattt taccgcaggt tcagaccttg
      661 cgtgatttgc ttgaccggaa ctgtctcgtt acagttgtgc aggaaacgga gctaaaatcc
      721 gcattggata agacaaaaga acccccttct ctggtaataa ccgattccca ggtaatagaa
      781 atggtggcaa atattgtgcc tgaagaaatt cccctaacta ctttttccat cctttttgcc
      841 cgctataaag gtgaattgga cattcttctg gaaggcataa accgccttaa aacactgcaa
      901 gatggagata aaattttgat agctgaggcc tgttcacatc atattcaaaa agacgatatc
      961 ggtagggtta aaatcccgaa ctggatgcaa aaattcagtc aaaaaaacct gaattttgag
     1021 gtctatgcgg gacacgattt tcctgaaaat ctggaagaat acgctgtttg tatccattgt
     1081 ggtggatgta tgattactcc gatggaaatg aacagaaaaa ttatggaatg ccgtagacgc
     1141 ggcgttcccg ttactaatta tggaatgaca atatccttgc tgaaaggcgt tttagaccga
     1201 acaattaaac cccttttaaa agctaaggtg aaacaaacac aatgaaaatt tccgctttgc
     1261 aatttgcccc acgtttactg gataaagaat acaacctgaa aaaaatgaaa cagttgctta
     1321 gcgtactgaa aacagacctg gttgtcctgc ctgaattatg taatagtgga tatgttttcg
     1381 ctaataaaga agaggtcttc tccgttgcag aagaagcagg aaaaggtgag acatactttg
     1441 ccctaagtga aatagccagt tgtaataact gtaatatagt ttatggatat cctgagcttg
     1501 atggcaataa cctttacaat tcctgtatgt taataatgcc tgacggcaga ttctataact
     1561 atcgtaaaac ccatcttttt aatcgggaaa aactcttttt cagtcccgga gataccggtt
     1621 ttcaggtttt tactattttg aatggagtaa aaatcggaat gatgatctgc tttgactggc
     1681 aatttcctga ggcagccaga actcttgctc ttttaggagc tcaaataatc tgccatcctg
     1741 ctaatttagt tctgccttgg tgtcaacagg caatgaaaac ccgttgtttg gaaaatcggg
     1801 tatttgccgt aactgccaat cgtataggca cagaaataaa tgcgggagaa acagttagct
     1861 ttactggaca aagccaaata ttaaatacca gaggtgaagt tctttcctct ctgtcagtta
     1921 gcgaagaaag aactataacc gtagaaataa atcctgaaga agcaaacgac aaaaccgtaa
     1981 ctgaacgcaa tgatgccttt aaagatagaa gaccagagtt ttataaatta tgaataaaaa
     2041 tgaattaaaa gccagaattg accgcttaag agccgaaata gaacatcata atcggctcta
     2101 ttatactctt gccaaacccg aaatcagcga ttatgaatat gatatgctgg tgaaggaatt
     2161 acaggaattg gaagcacaat atgcagaagg tgcaattgaa gaatcaccta ctcaaaaagt
     2221 tggaagcgac cttactccag gcgctaaaac cattcctcat aaagtgcgga tggcaagttt
     2281 ggataatgct tattctttgg aagaggtttt ggctttcatc aatcggacaa ataatgattt
     2341 gggttatgcc agtgaatact gtgcggaatt aaaaatagac ggatttagca tcaaccttta
     2401 ttatgaaaac ggaaatttta tttatgccac aacccggggt gatggtttag aaggtgaaga
     2461 tgtaacagaa aattttcgtt tgctcccgga tgttcctttt accattgatt ataaaggagc
     2521 catagaaatt agaggcgaaa tatatatacc cgtgcaggat tttctggcgc tgaatgaatt
     2581 ccgtagagaa aatgaattga agacctttgc taatccgcgt aacgcagctg ctggttcaat
     2641 taagctaaaa aatagagagg aagtgaagtc ccgtcatcta aaagcattgt tttatgcttt
     2701 gggctattat gagacacctc tgcagtataa aaatgaaatt atagataagc aaacccggtt
     2761 attggacttt ttggatgaac ttggtttccc aacaagtaaa agaactttat gcaagaacta
     2821 tcaggaagtg gaaaaattct gcctgcaaat ggaacaggaa cgttatacta ttccctatga
     2881 tattgatggc attgtgatta aggttaatag tttggaagat cagaaaaaat tgggctggac
     2941 ggctaaaagt cccaaatggg caattgccta taaatttaag ccggaagaaa aagaaaccat
     3001 tttggaatcg gttagttttc aggttggcag aaccggtgca gttactcctg tagctaatct
     3061 aaagccggtt tttatttccg gcagcactgt ttctcacgct actttacata atgccgatga
     3121 aataaaacgg ttggatttgc attatggcga tactgttttg gtggttaaaa gcggagaaat
     3181 tatccccaaa atcctttctg taaatgtcag taaacgccct gtaaatgcca aaccggttat
     3241 ttttcctgca acctgtcctg tttgtaatgc acctttacag cgcgatgaag atggcagtat
     3301 aaactactgt cctaatgcca attgccccgc tcagatacag cgtaaactgg aacattttgc
     3361 ctcgcgtgaa gcaatggata tttatggctt aggcagttca ctaatagcgc gtttgataga
     3421 aacaggtcag attaaatgga tacctgatat ctatcatctt gattataata aaattgcaga
     3481 aatggaaaga atggggaaaa aatctgccga taacttgcaa aaagcaattg aagcatctaa
     3541 aaccaaaaat tttgacagag tccttttcgc tctcggaata agacatatcg gcttaattac
     3601 tgcccgcact ctggctcaac actttaaaaa tattgatgct cttattatgg caaaggaaga
     3661 agaactctta agcgttcccg atattggttc cattattgcc cggtccctta tcagtttctt
     3721 ctcccaaaaa agcaatcggg aattgattga ctctttgaga aaagtcggat tacaattcac
     3781 ttacataagc ccgcaaagtt ctgctatctt agctggaaag acctttttaa taacgggaac
     3841 tctgcccaac tatagtagaa aagatatgga aacactgatt actgaacacg gaggaaaaat
     3901 tttaagtgga gtaagtaaac aattgaatta ccttatagta ggtgaaaatc ccggctccaa
     3961 actggataag gcacgccaac tgggaacggt taaaattatc agtgaagcgg aacttttgga
     4021 tatgataaaa tgaaaatatt gaaacgctat atcctgaaag agcatatcgc accttttttt
     4081 attgctctga tggtagttac ctttgtgctg ttgagcgaca gaattattga cttaatgaat
     4141 ctgataattg aaaagaaact gccctggcaa acggtttttg aggtctttgg tctttctctg
     4201 ccttatatgc ttgctctttc cattccaatg gctgtgctgg tagctacaat tcttgctttt
     4261 ggcagaatga gtgtagatag agaaattaca gctattaaat ccagcggtat aaatgtttat
     4321 tctttaatcg gacctttggt gctcgctgcc ttgctattaa ccggattgat ggtgtatttt
     4381 aaccattggt ttttgcccga tactaaccat aaattgaaaa accttatgtt gaaaattgct
     4441 tactacaagc caatgacaat tatcaaagaa aaagaattta ccaattttat ggattataca
     4501 atatatgcca gagaaacaac tgatagcttg ctgacggatg ttttaatcta tgatcgtagt
     4561 cagtcacatt ttccacgcac catttttgct aaaagcggta atgtgattca aaaagataat
     4621 ggcaattctc tccaaataat tttgaatgaa ggtgaaatgc atgaacgcaa cgaaaaagaa
     4681 gtcggtaaat accagaaaac taccttcagt cgttatataa ttaatgtgcg taacctgggc
     4741 agtaatatgg aaatgtttga aactggctac agaagtgacc gggaaatgac ttataatcaa
     4801 ttgattacag ctattggaga acacaaaaaa gaactgcaga caaaacagca ggaaattgaa
     4861 gaattgaata aacgcattga attactccaa aaaagcccgc agaattttac ccgcaatatg
     4921 gaactgcgtc gtttacaggc aatgcaaaaa atggtaattg accgcaccca ggagctgaaa
     4981 gaattaatca actctttact agtggaatat cataaaaaat tctctatgtc ctttgccatc
     5041 attatctttg tcctgattgg aattcccctg ggtttgatga ccagaacaag cggaattgga
     5101 atgtcttttt ccgtttcttc tataattttc cttgtctatt atattgctct taatggtggt
     5161 gaacaacttg ccgataaagg atttatgcct ccttttttag ctatgtggct ggctaacatc
     5221 atatttttaa ttttagcagt tctgcttata ataggttcca taaaagagaa acggcttctg
     5281 gatatgccta ctctgatatg gaaaattaaa cattatagga atagaaaagc tcctgttccc
     5341 gatgagattg tgcactaaaa atgagaattc ttgataaata tatcatccgt gaatttctgc
     5401 gcacctacct aatcatcttc ctttcttttg tagctatctt tgtggttatt gatgtaattg
     5461 acaatcttcc gcggttaatt cgcaatggag ctaccgctca acaggcaact atatactatt
     5521 tgatgaggtt accttatctg cttgtgttaa cttctccggt tacggttctg ttagccggtt
     5581 tgtttatgat gagcgcttta tccaaacata atgaaagtat cgctattcgt gctgcaggaa
     5641 ttagtataaa aagagctatg ctacctcttt ttggaattgg gcttataatc agtattgtag
     5701 ttgccgtatt tggagaatat gtagtccctt gggctgaaac tactaaaaat tatgtttata
     5761 atgtccaaat caaaggtcag caaccggatg accaaatgct gaaagcccga atccactatc
     5821 agggtaaagc taacgatttt tactatttcg gtttctttga tggctataaa aacaacctaa
     5881 aaattattga ccttacccgt attgatttta aaaataaaca aataactgaa catataactg
     5941 ctgcctctgc ggactggaat ggttctcgct ggattttaaa ggattgtgat attagaagat
     6001 ttgccaatgg taagcaggtt tttatggctc attatccgca aaccgattta gttattctgg
     6061 atgttcagcc ccaggatttt atccgcatat ctcaaaaaac actctctctc aatttttggc
     6121 agttaaaaga atatatcagc aggatgaaaa aactgggaga taatccagca agagaaattg
     6181 tggatttaaa tatgaaaatt tcctttccgc ttaccaatct catagttatc tttttcttca
     6241 tcccgattgc cacttccaat attcgctcca aaggacgcgg ctggataatt atgctcggtt
     6301 tggttgtctg ttttatctac ttaattgtgg taagggtttg ccaaagtttg ggctacaaca
     6361 gcgttatcaa tcccttctgg gctgcctggt ttcctaacat cttctttact cttttgggct
     6421 tgggcttcct ctataaagcc gaaatttagt gtagtcggag ctcgccgaag ctccgcatct
     6481 ttaaacgagg tattgaatgg atgtaacttt tttagcggag gattggcatc ctcctgctac
     6541 acaaacatca gaataataac attctgtagg tctggcttta ttggcacaag taaaattgca
     6601 taagtaacaa aatcataaag gtacaaataa ctcctgactt tcaataattc aattaccctg
     6661 ttttcattaa ctccattgct acaattactg cttgacaaac agccaagtac aaaaattgag
     6721 gatagaataa ataatagtta tgtgaggtta ggttctaaaa ttaaaaatga aaaaacctaa
     6781 cttccttata taaggagttt tactttgttg gcagataaca atcgtattgc cattcatcct
     6841 atattgccga ttcccgaaag agaagaggtc tttttttact ggaatggaca aaaactaaag
     6901 gcaaaaagtg gagaaatgat tgcctcagct cttattgcca atggtatatc tatttttggt
     6961 catcatcata aagatggcag tgcacaaggt atattttgtg ctaatggtca atgttctcaa
     7021 tgcagtgtaa tagctaatgg cgtagctgta aagtcctgta tgacagctgt tagtgaaaat
     7081 atgattgtgc aatcgctgga agggctacct atccttcctg cagaagatac acccttaaac
     7141 tttgaacctc tggaatatat taaaaccgat gttcttatta tcggtggagg tcctgcaggg
     7201 ctttctgctg ctattgaact gggcaaacag aatatccaga cattgttgat tgacgataaa
     7261 aatgccttgg gaggaaaact tgttttacaa acgcataaat tctttggttc gcaggaagat
     7321 agcagtgctg ggaccagagg tattgatatc agcaaaaatc tctctgagga acttgccaaa
     7381 ttcggcagtg tagaaatttg gctaaatagc acagctgtct tcgtgttcag cgataaaaaa
     7441 gtaggtattt tgaaagacgg tgtctataaa atagttgaac ccaagcgtat tttaaacact
     7501 gcaggagcaa gagaaaaatt cttaagattt ccggggaaca atttagccag aatttatgga
     7561 gcaggagctt ttcagacctt ggtaaataga gaccttgtgc gtcctaccaa tcgtcttttt
     7621 attattggcg gtggcaatgt tggtttaatt gcaggttacc attcattgca agcaggaatt
     7681 gaagttgtag gtttggcaga agcaatgcct gtttgcgggg gctataaagt tcattctgat
     7741 aaactgaaac gctttggagt gccgatttat acttcgcatt ctatactttg tgctaatggt
     7801 aaagaatcgg ttgaaagcat cactattgcc gaaattgata aaaactttca acctatacct
     7861 aaaacagaaa agaccttcgc ctgcgatact attttaatag ctgtgggctt aaatccctta
     7921 aatgaattca cttgggaagc aatggatgca ggaattcctg tggaagctgc aggtgatgct
     7981 ttagaaattg cagaagcaag ttcagcaatg tttaacggta aaattgccgg agtaaaaatc
     8041 gctgcagaac tgaaaggaaa taaagataac cctattccga aggagtggta tgccaaagca
     8101 gaagagctaa agtcgcctcc gggaattaca aaaagctatc agaccccgga aaaggaagaa
     8161 ggtgtattcc ccgttttaca ttgcttgcag gaaattccct gtaatccctg cacaactgtt
     8221 tgtcctacaa atagtatcaa aactgaggat ggttcactga tggctgttcc tgtttatcaa
     8281 ggttcttgta ttggctgtgg aaaatgcctt ttgatttgtc ccggtttagc tataacctta
     8341 gttgactacc gaaaagataa agaaaatccc acagtcagca ttgtttacga agtaaccaac
     8401 tatcctgtcc aaatagggga taaaaaaatc ctgaacgata tagatgccaa cgaattagga
     8461 gaatatgcca ttaccgctgt tcaggatttt cccaaacagc atacccaaat agtaaaattc
     8521 caggtgccta aggcaattgc taaaaaagta gccggtttta gaattcagga tgcaagtgtc
     8581 tctgccccaa tagataaacc aattattttt gaaaagacat ccgatgaggc aatgatttgc
     8641 ttatgtgaaa gagtcagtgt agggcaagtg cgagccctta ttaaaaaagg tattacggac
     8701 ttaaatctga ttaaagcgat taccagagca ggaatgggac cttgtggttc caaaacctgt
     8761 gaggttttga taaagggttt gcttcgtgaa gaaggcattc ctgaaaagga ggttgttgcc
     8821 aacaccaaaa gacctctttt tatagaaatc cctttagcaa aatttcctga cgggagcgta
     8881 aaatgaagaa tatctatgac ataattgtaa ttggagctgg ttcaattgga ttgccgaccg
     8941 cttataattt agccaaagcg ggaatgaagg ttttagttct ggaagcggaa catagtcccg
     9001 gacagggaaa taacaaaaaa gccatcggtg gagtacgagc tacgcattcc gattttggta
     9061 aaatcaatgt ctgccttcgt tctattgaaa ttttgaagaa ctggcaggaa gaaacgggtg
     9121 aagatatcgg ctggctttcc aatggctata gttatcctgc ctataatgaa aaagatgccc
     9181 aagaattgca agacctgatg aagatacaac atagttttgg acttaatatt aaatggctta
     9241 gccctgatga atatagtgaa atcgttcctg gtattttgca agagggcttg cagggttcaa
     9301 cttattctcc ggaagatggt tcttgttcac cattacttgt ttctgctgcc tattttgaac
     9361 attcccaaaa agcaggagtg gaattccatt ttcaggaacg agttaccggc tttgacatta
     9421 gagctggcaa aatttataaa gttattacta ataagggtat ttattcctgt gcaaaagtgg
     9481 ttaatgcagc cggaagttat gcacgcgaaa ttggcaaact ggctaatttg gatttacccg
     9541 tttttccgga taatcatgaa gcagggatta ctgaaccggt agaaaggttt tttggtccta
     9601 tggttataga tatgcgttta gcaccaggaa gtgccaattt ctatttttac cagaatattg
     9661 aagggcagat tgtattttgt ataactcccg aaccaccgat tgagggaatt gataatcgtt
     9721 ccacttctgt ctttttaccg atgtgcagta aaagaatgct gacaatttat ccgcgtttac
     9781 gcaatttgaa agtgcgcaga acctggagag gacagtatcc tatgactccg gatggctttc
     9841 ctttagtagg aattatgcag gaggtggaaa accttgttaa tgcagtagga atgtgtggtc
     9901 agggctttat gcttggtccc ggaatgggag aactagtaac ccgtatttgt ttggaaagtg
     9961 ctacagataa ggatttgaag atattggaga gctttgatcc ttatcggcag tttacgagtc
    10021 aggaagcgtt taaatagatt tctattacaa agagaaaagt gagagtttag ccactaaagt
    10081 caccgaaaag gaatttttgc agaagaaaga atagagagaa agattattga ataatttcgg
    10141 tgttctcagt aacttctgtg tgctcggtgt aaaataattc cttttcactt ttcacttttc
    10201 accgttaacc tgttcactat ttctactatt tctttcggtt catttacaag gtagtcgggt
    10261 ttatagcttg ccaaaaattc agctgtatga aatccccagg taactgcaat gacttttaat
    10321 ccgcaattat gggctgcttc aatatctctg atttcatctc cgatatagat tacctcattg
    10381 gtagatattt tatgtttttt taagcgtttt ttaatatctt tggctttatt gagaatgccg
    10441 gaagttcctt caatccacaa aaaggaatca attttatgat tagtaagaaa gagatggaca
    10501 ttttccacgg tattagaact taagagagcc ataggtatct cttttaaagc aagttcattt
    10561 atcatttcaa caattcctgc acacggttca agttcagaaa taaggcgttt gtattcttta
    10621 aacgcaattg tgacgatttt tggtattttg taaaaaggga tttgcatttc tctggctatt
    10681 ttatgccagg ttaaagagcg aaagtgctga atatcatttt ccgtaaaggg cgggtaacca
    10741 aattgaggag caagattatt ggcaattttt aaccccagct gaagagaatc tgctattgtg
    10801 ccatcaaaat caaacagcag aaagggtttc attttcccct ttccttttta acactaatta
    10861 ccagctggta tgcatctttt aaagaaaggg aagattttaa acaaaccata tctcttatat
    10921 cttttgtctt tagtcccaaa tccagggcgc tatttatata attaataaga tcgtcttcat
    10981 ttaattcttc ttgttctttt tcctcttcac ctttaagttt ttgaccatct aaaattaggc
    11041 aaaattcacc ttttatagtg agggacgggt ttttattaat ttcttctaaa gtagtatgga
    11101 tatgttcttc gtgaagtttg cttatttctc tgcaaataca tatttttcgg ttaccgcaaa
    11161 ccgcataaag ttcatttaac aatttccaaa tacgatgagg tgcttcatag ataactgtag
    11221 tataagggta ttccgcaatt tgttccagat tttcccgtct gcttataggt tgctgaggta
    11281 aaaaacctac aaattgaaac tgtctggtag aaaaaccgga tacacttaaa gcaggtaaaa
    11341 ttgcactggc tccaggcaaa gcaaaaacct ttattccttt agcaattgct tcctttacaa
    11401 tatgttccgc ggggtcactg atggcaggac ttcctgcatc agaaacaatc attaaatcct
    11461 ttccagtttg caggtattca aaaaaatatt cttccctctg tttttcattg aatttatgaa
    11521 agcttatcag tttcggaacg ggaattttgt attgttgcaa gaggaaagct gtaactcggg
    11581 tatcttcaca ggcaataagt tccgcttttt ttagcagttc cagagctcgt agggttatat
    11641 ctcctaaatt tcctataggc acaggaataa ggtaaatatt tccggagggc ataataaata
    11701 aaaaaaagct aagtgccggt aagtaaaatc cggcacttat aaattagctg ttaaattaaa
    11761 ccgagttctc tgccaatttt cttaaaggca gaaatcgtga aatccaaatc ttctttagtg
    11821 tgggcagcag aaagctgaac ccgtatacgc gctttgcctt tggggacaac aggataaaca
    11881 aagccaatta cataaactcc ttcttctaaa agcatatccg ccatctttat agctaaaggt
    11941 gcttcataaa gcataatagg aacgattgcc gtatttcctg gaactatatc aaaacccgca
    12001 gaaaccattt ctttccgaaa ataacttgca ttttcccaaa ccttatctct cagttcatta
    12061 gaatgagaaa gcatttcaag aacctttaag gtggcaccta ctatagaagg agctaaagta
    12121 ttggaaaaca aataaggtcg gcttctttgg cgcaaaagtt caattatttc tttgcgtccg
    12181 gaagtgcaac caccatttgc tccacctaat gctttaccga atgtggtagt tataatatca
    12241 attctatctg taacgccaaa atattccgga gttcctcttc cgtttttgcc aatataaccc
    12301 gtggcatgag aatcatcaac cattaccaaa gaatcgtatt tatcggcaag atcgcagatt
    12361 tcatccaact ttgccatatc tccatccata ctgaaaacac cgtccgtaca aataatacga
    12421 taagtgcaat cctggctttc ctttaaggca ttttccagct cttccatatt gctatgttta
    12481 tagcgcaagc gttttgcctt gcaaagacga acgccatcaa taatggaagc atgatttaat
    12541 tcgtcggaaa taattgcgct atcttcaccc aaaagaggtt caaaaagtcc accattggca
    12601 tcaaaacagg cagcataaag aatggtatct tccattccta aaaacttgga cattgcttct
    12661 tccagttgtt tatgaattgc ctgtgtcccg caaataaaac gcacggaatt acatccataa
    12721 ccccaatcat tcataatatc ttgtgcagct ttgattactt caggattatt acctaacccg
    12781 agataattat tagcacaaaa gtttaaagct tctttaccgc cttgaacacc aattcttgca
    12841 ccttggggag tggttaaaat gcgttcttct ttgtaaagtc cttgttcttt gagctcttta
    12901 aagagttctt gcaaatcgga tttgattttt ccgtaagcca tttgattcct cactttataa
    12961 taagtttttc tttgtttaac ccaaattata agttctttta tttcgtcaat ggaatttatt
    13021 tttctttccc aactcaccat cacactatct caccatttca ctttctaact taaatgattt
    13081 tacttgacag gatattgcat ttaagtatta tgtaattata gaggtaataa tgcaaaagat
    13141 aatcattatt ctatgcactc tggtttgcct cagttttggg gctgaagcca tttctatata
    13201 tgaaattcag tttactgctt cagcaggcag tgacggaact tttccctctc catatttagg
    13261 taaaaccgta tccacagaag gaattgttac agctatggat ttcaaggaag gcggttattt
    13321 tatcagcgaa aagttaaatg gtccctggcg gggaattctt atactggata gaaatccaaa
    13381 agttaaaaag ggcgatttta taagagtttc cggcatcgtt tccgaatttt acggaatgac
    13441 ttgtttacaa gatatttctc aaacaaaggt catcagcagt aataatcctc ttccgcaacc
    13501 ggttctttta actacaggtc agttatctaa tgcgggagaa gcagaagcat acgaaagtgt
    13561 ttatgtccgt tttttgaatg ttacatctgc aggcaacaaa tctcttaaaa atcgtttcac
    13621 agtaactgat ggcagtggca cttgtcttgt tttgaataat caatttggca tagagcgcaa
    13681 taataaaatt gccttaaatg ctcaatattc gtctctcaca ggaattgtaa tttacggcta
    13741 tagcgaattt gccctttgtc ctatttctac atcggactat gttatccagc agccaatgtc
    13801 tgttcagaac cgttcctggg gtagaattaa atccatctac agataaaaaa taaactaatg
    13861 aggtaataac aatgaaaaag tttttcaaaa aaatcctgat tcttggtggc acacttgccg
    13921 ctgctcattt tggctttaag gcatataaaa aaattagcgg aactgctaaa ctttccaaat
    13981 cattgcctga atttttgaat aatgtttatg gcgaaatgcc acaggtaaat gtaaatcaca
    14041 ccctaaattc tactcgcatt aaggtcttct tcagccagga aattctggat aagcacaccg
    14101 atatagaaac tacgattcgt gaatatattg atgattttta tcctgaaatt gccaagacct
    14161 ctgtagatat cagtattatg cccgctactg aagaaacaga agatattcct gttgaagaag
    14221 aaactgaaga aaaagaataa gcccttattt atccctgatt attcccaaaa cccggtttct
    14281 aaccaaaacc gggtttttgt gtagctggag gataccattc ctccgcaaag aaaaaaattg
    14341 tggagcttca gcgagctccg actacccggt aaaaggtctg taatttatag atgttttttt
    14401 gagagttaac caattcggaa atcctaaaag tttcgttttc agttagtaac catccaagaa
    14461 attattattg ctccctacat cctctaataa ttatataaca tttctgtaac gactccgtaa
    14521 cgagataagg gaagtgttac ggaagtgtta cggcagtgtt acggggttga cttggaacga
    14581 attgataatc aataaattgc gaatatacaa tttaggggaa atcggaggag aaaattattc
    14641 cttcatttaa cgaggggatt acgccttcat cgctattatt gtgttgccat tcggactttt
    14701 agcaaagggc aaagagcgaa gaacaaaggg tttttggtga ggtgtcctga aaatagcgga
    14761 ctaaaattaa ggagaaaaaa atgaacaaaa attctaatga aaggatgcaa gtccgctaca
    14821 gcgatgcctt caaactgaag gttgtacagg acatcgaaaa aggaaagttg acaataagtg
    14881 aggctcgtcg tctttatgaa attaatggta ctcacacggt ctatcgatgg ctgcggaagt
    14941 acggaaaaaa cgatttaatt caaaaaattg tgaggatcga aatgaaagac gaaaaaagcc
    15001 aactcaagaa aaaagatgaa cggatcagag aattggaatc cgttattgca tctttaactc
    15061 tacataactt atgcttgcaa aactatgtag atgtagtgaa tgagaacatt agtgaagagg
    15121 aaaaaaaaac ttcactttcc aagctgtctc cagagcaaaa gcaagtgctg atgcgacttc
    15181 tatcaaaagc tcgatgatca aaatctgtag taacttaggc gtcagtaagt cctggtatta
    15241 tgaaactatg gctaaccggg aaaaagtacg acaatatgaa caacaaattg ttaatgaagt
    15301 ccaagaaatc agaaaggaac atcctgtcta cggaaccaga aaaatctggc atcaacttgc
    15361 cagagacgga accataattg gccgggataa acttcataaa atcttgctga aatataatct
    15421 aactcaacct aaacaccgaa aaaaagtgcg cactaccttt cctggtatgt atgaacaggg
    15481 ctttattaac ctaatcaaga acctgaccat tgaccataaa gaccaggtct ggtgtacaga
    15541 tatcacctac atttacacta cagagggaat attgtatgtt tcgggaataa tggatatctt
    15601 ttcccgtaag ataataagtt tcaatgtcag taataaccta agaacagaag gatcgctggt
    15661 ttgtttgcag aaagctttaa aacaggtagc taatcctcaa ggcatcattc accattcgga
    15721 ccatggcgtc cagtattgtt ccggaagata cctggaagct ctgcttagca aaggaatgca
    15781 ggttagtttt acaggaccca atcattgctt tgataatgct aaaatggaac gtttcttcaa
    15841 taccctgaaa tatgaatatg gtctcaatgc ggtaattaaa tccaaaaaac tggctatcca
    15901 aatgattaaa aatgcggtgc aaaattataa ctatacccgc ctgcatgctg ctattaacta
    15961 tatgataccc tgtgagttat atgatgtggc ttaggataaa gaaaaaccta aattgccctc
    16021 tgaaccaatt tactgaacag aaaatgtgct tgacaaaaaa cctgtctttg ctaatggaaa
    16081 aacaaattcc ggcgaaacag aaacaggctc tgctgggaag caacctgcca gggataaccg
    16141 ggaccaataa gggtgtgaac aaaagtgagc gtaacgattt agttatgctc acacccgttt
    16201 ggggcatgca taagaaaatc ccaaaccagt atcctctgcg taccttgcta aaccttaaaa
    16261 atagcgaatt acacaaaccg ataaatggcg ctttataccc aatattcttg tcttatattg
    16321 gcttaatatt gttaacatat aataatcaag atcgattaca aaatattatg tgccatttat
    16381 ctcaaaattt aaaaatgaaa aaacccaaaa aacagtccgc cattttagga cttgacatgg
    16441 gtcattttta aaaagaggta gatgggaaca ccaatcttcc aatgttactt ttcctgtttt
    16501 taaggaatct tatccttcta aaccccttca actttctaaa ctttgtaatc cctttttcca
    16561 acctgcgttt taaataattg taaatccgtt ataatcggta aaattcgtgt tcttttcttt
    16621 tatctttgtt atctgtgaaa tctgtgagga ttattctaca gtaacgcttt tagctaagtt
    16681 tcggggttga tcaacattca aaccctttaa atcagctaca ttatatgcca gcaactgtaa
    16741 aggaataacg gttaaaaggg gttgcagatt agttaaggta tcgggaatat aaattacatt
    16801 ttcagatatt ttagatagct ctgtatcgcc ttcggtagcg atagttatca accgtccttt
    16861 ccgagcccga acttcctgta aattggaata aattttatcg tagaggggat cttttgtggc
    16921 aatagcgatc acaggcatat tttcatctat taaggcaata ggaccatgtt tcatttctgc
    16981 ggcgggatag ccctcagaat ggatataaga tatttctttc aatttcagtg ctccttccat
    17041 tgccacagga taatttattc cccttcccag atacagtgca tttttgtagt ccttaatagt
    17101 atcggcgata ttccgaatgt attcattaag tgccaatatt ttttcaactt tggaagggat
    17161 attttccagt tccgaaatat attccattcc tttaacggta gagagataat tcattcgtcc
    17221 caataaaatt gccagcaagg tcataatcgt aacttgcgaa gtaaatgctt tagtactggc
    17281 aactccaatt tcactacccg catggatata agttccgcca tcgctttctc tggctattgt
    17341 actaccgact acattggtta tggctaacac tctggcaccc ttcgctttgg cttctcttaa
    17401 agctccaagg gtatctgctg tttctcctga ctggctgatt acaaagacca aagtgtcttc
    17461 tggaatgata ggattccgat agcgatattc tgaggcatat tctgcaaaaa caggtatgcg
    17521 agctatatct tcaataatat atttacctat taaagcagcg tgaaatgaag tcccacaagc
    17581 aactaaatga atttgtttta tttttctcaa ttcaaaattg ggaattccca acccccctaa
    17641 acgcgctgtt cccaaatgtt gatttattct gccgcgaaaa gcattttcaa tagttaccgg
    17701 ttgttcaaaa atttctttca gcataaagtg cttaaaatca cctttttcaa tagcgctaat
    17761 atcccagtcc accactgaaa tttcgggctt tacactgcgt ttatccaagg tcgtaatttc
    17821 aaaaccatca gctttaacaa cgcataattc atcatcctgt aagtaaataa ctcgctttgt
    17881 atgaataatt atagcattca catcgctggt tataaaatgt tcattttcac caataccaat
    17941 aataagagga ctacctttac gcacggcaat cagcttatcc ggctctttta aagacataac
    18001 tacaagcccg taagtgcctt caacttttgt catagcagag cgaacagcat cttccaaagt
    18061 tggttcactt aaaagaaact gttcaataag atggacaata acttcagtat cagtttcgct
    18121 tataaaagta tgacccaatt ctaataattt atctctcaag agcttataat tctcaataat
    18181 accattatgg acaatagcaa tttcaccttt acaatccaaa tgtggatgag catttacttc
    18241 atttggttct ccatgcgttg cccatctggt atgtgcaatg gctaaattgc cggtgcattt
    18301 attgggttct ggcaaacagc gttccagttc aataattttg cctgctcgtt tataaacctg
    18361 taattcgcca tcatgaataa gagcacatcc ggaactatca tatcctctat actccaatct
    18421 ttttagtgct tcaacaacaa taggaagtgc attacgatga cctatgtaac caactattcc
    18481 acacatttat cttctccttt ggcagtaact ttatgaaatg cataaagaga taaacaaaat
    18541 ataatcaaag ccggaacaat tagccaataa tttagatttt ctccatcttt ggctataaat
    18601 tttacccatc cggtattgct gtaagttacc agaacaaaag ccattccgtt aattataata
    18661 tgcgaaaaca tactggtata aattgagccg gaacggatag ccagataacc taataacaat
    18721 cccaaaagca aaacaggcac aaaacgaaaa ggatcaagat gataggcagc aaaaagaatt
    18781 gcgctgatta caacagccca gcgcactgaa tacttttcaa aaaagcggat tataaatccc
    18841 cgaaatagca gttcttcaca gattccaggc aataaagcta ttgccataaa aaccttccaa
    18901 ggggtttcat tcattttata taattgggaa agtgcttcca gatatttttc cggaaaggga
    18961 aaaacgatat taatcaactg cgaaattata gacacaatta tttgggcaga aacagctata
    19021 aaaggaatta agagaaagct attccaattc gggtttttta atcgtaaaac ttctttgggc
    19081 tttaggcgta aaaaccttaa aattaacaaa accggtaatg caatgattat aatttcggtt
    19141 tgaatcagcc cgctgaaaag gtcttttgtc tgccagtaac tgccaagata atataacaca
    19201 attaaagcaa tagaaaaata gagaattcca ttgtagggat taaaaaaggt tttgggcttt
    19261 ctcttaaaaa ctttcaaacc accgctttct tcttctgtcc taaagagaac tgattccgtg
    19321 ttaaacagct ttatcgtaat aataatagca ataacatcca atacaacagt gctgaaaata
    19381 gttaaaagta tttgagaaat agtaaattca ttgatcaaaa cacctttaaa cagcagagca
    19441 atattaacaa taggaattaa agccatcaga ttgtttattt caattgccgg aaagaaactt
    19501 accattccca aaagcattgc tattatcaat aagggctgtt cataacttct tgcctctttg
    19561 atattcctgc tgaaagtgga aatggataac agaatagcgg caaataaagt tgctaaagga
    19621 atcatagcta tcagcaaaat caatatagca ttaagtggta aggaaacccc tgccatttct
    19681 gtgctttgat tagctaacat aaattttagg gaaaaactga tactgaaaag gtttattatc
    19741 atattcaaca ttgccagggt aatgatcgtt aaatatttac cacaaacaat ttctaaacgc
    19801 cctactgaag acacaagcaa ggtctccaat gtttttcttt ctttttctcc cgctaccaaa
    19861 tccgcagcta caatagatgc acccgcgaat aacatcataa taacaatata tggtaaaaac
    19921 atacccaaga gcatacccat tttcttttgc gcactggaag tatcgcgttc ccgaatatca
    19981 atcaggttca aaaaatccgg actgatacca aaactctgta acaactcttt ctgcaattcc
    20041 ttttccgttt tagaaagttg ttcggaaagc ttgttaaaga ccattctgct ttgctctttg
    20101 gatttgtcat attgaatata aaccttgaaa aattgcgttc cgtcactgcc cagagaatca
    20161 cgaatggtaa cgatgcactg aatatcttta tcttcataaa gccgggaagt agtttcgctg
    20221 tatggtataa tggtataatt tttaatcttc gttaaatcct gaatcaattg acgggatata
    20281 ctattattta cgctatcttg aacggcaaca gtagctcctc tttcttccag tgatttggtt
    20341 tgacggatca ttattgaatt aaaaccgaca atgattaaag gatataaaat aataggaagg
    20401 atgaaagtag taaaaatggt tcttttatca cgcagaactt ccatcagctc ttttctgtaa
    20461 attaccaacg agtgtttaaa attcatttgc tctcaactcc gtttcatcgg tgtattgatt
    20521 gatgtaataa acaaaaatat catccagatc agtttgagaa gatgtattgc gtaaatcctc
    20581 cagagtgccg tccgctaaaa ttttcccttt atgaatcagc acgattctat cagccaaccg
    20641 ttccgcttct ctcataatgt gtgtagaaaa taaaacacat ttcccgcgct ttttacaatc
    20701 ccgcataaaa gagataatat ttctggaggt taaaatatcc aagccagctg ttggctcgtc
    20761 aaaaatcatt accggaggat aatgaataat agaacgcaca atagacacct tttgtttcat
    20821 cccggaggac aaaaattcta tctttttatc cagaaattca ttcatatcca gtaaagtagc
    20881 catttcgctg atccgctgtt tgataatatt gtctgcaata ctgtaaagac gaccgaaata
    20941 agtaataaac tcccttccgc ttaagcgatc ataaagtcct gtatcaccag aaagaaaacc
    21001 aatattttct ctcactttat taggatgttt tttaatatcc caaccctgaa tttcagctgt
    21061 tccttccgag ggctgaaaaa tagtggaaag aacacgcatt gtggttgttt ttccagctcc
    21121 gttaacacct aaaagacaaa caatttcccc gtcaccagct gtaaaattta ttttatctac
    21181 agcataaaag tttgttccgt cttttttggt aaagaccttg cttaagtttt ctactttgat
    21241 catacacttt ccccttatca gattactaaa gcacaatttt gcaaggttaa atttctgtcg
    21301 aggtttttta tttccttttc ggtaaagaaa tatcgctaac taatataagt tccacattca
    21361 aaattcagtt atataggtaa aaaaatatcc gaattcctac ttttgtggga aggacaaaag
    21421 aattttacaa aaaggaaatt gatataaaaa ccaaataaag cttgactatt tagctttatt
    21481 tagctttaag gggaaaaatt ggggatagat gaaatatgaa agaaaaggca actctcattt
    21541 taggtggcgg ttctgcattg ggtttagctc atattggcgt tttaagtgtt ttagaagaac
    21601 aatatgaaat aaccggtata ataggttcca gtatgggaag cataataggt ggtttatatt
    21661 cctcggggct caattctgag gagatatata agattgctaa aagtttcaaa aatacaagga
    21721 tattttctcc gctaaattta gaccgaacca tacaaggcat ttttgatggc aagtcattac
    21781 tgaaagcgtt tttgaaatgg acagaaggca aaaaaataga agagggcaga atacctttta
    21841 ttgcttgtgc ttacgattta atcagtaaaa gcaccgttct ttttaataaa ggactttatg
    21901 ctgatgcaat gagagcttct acttccttgc ctgttctttt tgctccttac aaatttaagc
    21961 agtatctttt tattgacggg ggaattgaac atcctctacc attagctttt tcttctgtgc
    22021 tttcccaaga taaagttatt gcggttaatg ttttgccgga agcccaaaaa agtgcctgct
    22081 ttatcaatct ggaaagtgat gaaataacca aatccaagcg tttaggtaga actgaagtaa
    22141 tctttaatgc agttttacaa aatcaggcat atctggcaat tcgtgatata gcagccttta
    22201 gtcctgatat tgtaattgaa gcaggaatgg cagatggtca tccttttgca tttcataagg
    22261 caagggactt ttataattat ggtcgcaaac aaacaataga aacccttgcc aattacaaag
    22321 aaccaaggtt tataactgca atccgaaagc actaccgtaa tttaatccgc tatctaccca
    22381 atttaaacat ccctgttaaa gataatactt gacggaaaat ggcaggcata aaattatagt
    22441 aattatagat taagaataaa aacgcagtcc tcagaactgc acaatcagag aagaaaaaat
    22501 agaggcaaaa atcccatatc aaaatacaag cgaggagttc tgttatgaat gaaatcatca
    22561 aaattctatc caaggtagat ggtggtaatg ttttggacgc agcaaccgga aaaggcgact
    22621 ttattacaac cctcaaacaa catctgaaat cttacaccca aattattgga gttgattcct
    22681 cgcaacgctg tgtggattat gcccagaaaa tattcccgga aaacaatata gaaattttcc
    22741 gtatgaattt ggaagatatg cagtttgaag ataactactt tgatatggtc tgtatgtctg
    22801 atgccttaca ccatattaaa aacagggaaa aagttctaaa tgaaatgata agagttttaa
    22861 aacctgaggg tttgatgtta attacggaaa tgtattgtga cggcaaacag acccctgctc
    22921 aggaaacaca tattataatg catcattggg ttgcctcggt agaccgcctt ttaggaactt
    22981 accatcaaaa gacatttacc cgcaaagaac tgcaggatat aattaaaaaa atgcccctga
    23041 aaaaagttca gataacggat ttctatgttc ctgtagatga tcccagcaaa aattgtccta
    23101 ctttgttgcg taactgtcag gaaattatta aacgtctgca gacaatggaa aacacggaag
    23161 cattaattaa agaaggacag gaattgatgc aacgcattaa agatatcggt tatgccagtg
    23221 cttgtcgttt ggctatcacc ggttacaaaa aaagtaatta taatgctata acgaatagct
    23281 gataaaggag ataacgaatg ctgtttgaag aagatctgtt tagcttgaat caacttcaac
    23341 taattcgcct ggcaaaaaaa attggtttgc ctggctatac acaatataaa ggaacggaac
    23401 taatctttaa gatgctggaa tttcaggcag ctcaagaagg gcttgctttt gtaacgggct
    23461 gtctggaaat tatggaagat ggtttcggct tcttacgctt tccccaaaat aactatactg
    23521 caggtcgcga tgatgtatat gtatcgctaa cgcagataaa acgcttcgga cttaaaaccg
    23581 gaaatatggt cagtggtcct gtccgcagtc ccaaagaagg agaaaaatat tatgctcttt
    23641 taagggtgga atcagttaat aatatggctc ctgactgtat gcagaatttg cgtccttttg
    23701 atgaacttac cccttattat cctacagaac gattaaatct ggaatttgat cctgataatt
    23761 acagcaccag aataataaat ctttttacgc ccattggaaa aggacagcgt ggtttaattg
    23821 tggcagctcc ccgaacaggg aaaacaactt tactgcaaga tacagcaaat gctattttaa
    23881 gtaatcatcc tgaagtttat ctcattgttt tactggttga tgaacgccct gaagaagtta
    23941 ccgaaatgaa aaaaatcctt aaacccggaa gcagggaagt tattagttcc acctttgatg
    24001 agtcccccaa aaatcattcc gcagttagcg aaatggtttt ggaaaaagcg aaaagaatgg
    24061 tagaactgca tcaggatgta gttattgttt tggatagtat cactcgtctg gcaagagcat
    24121 acaataatat aaccccttcc agcggaaagg ttttaagtgg tggaattgat gccaacggat
    24181 taattaagcc caaaaagttc ttcggttctg ctcgtaaaac tgaagaagca ggtagcttaa
    24241 ctattattgc caccgcttta attgatactg gcagtaagat ggaccaggtt atctttgaag
    24301 aatttaaggg cacaggtaat atggaactgg ttttagaccg cactatcagt gatatgagat
    24361 tgtatccagc aattgacctt gtgaaaagtg gaacccgacg tgaagaatta cttttaacgc
    24421 aaaatgagct gaaccggatg tatgtgttgc gtaaatattt aaagaccatt tcaccgattc
    24481 agggtataga gacattgcgg aaaaagatgc aggcaaccca aaccaacgaa gaattactaa
    24541 attcaatgag caattgatgc ttaaaagtcc ctttccagct gttaagcaag ttttcctttt
    24601 cattttgctg acgggattat gttgttttgc ttttgccgca gttagaaagg aatttcaaga
    24661 tttagacaaa ctatataaca gtggaaaatt agatgagcta tccgccaaca ttagcaccct
    24721 gaaacctgtt aatgatgaag agcgtgcctg tttgggcttt tataatgctt tgctgaaagt
    24781 gaaaattgaa gatgccatat cggcacatca gtggttaata gaaaaattcc ccaaaagtcc
    24841 ttatgctcag aaatctctct tggaactggg aaaaatattt atccttgacc gcaaaattga
    24901 agaagcaacc ttatatttaa ggcgcatcac ttctcccgaa atcatagaac gcttttattg
    24961 gcttggttta tgtgcctggt ggaatgatga ttatgcaaat gccatcagta attgtgaaaa
    25021 ttatttgcga ttagcacctc gcggtgaatt tgccgagaaa tcatattatc tgattgcaga
    25081 atgttatctg gaacaaaaaa aagcatacag cgcagttgct actttactaa aattgcaaaa
    25141 tgccaagctt cccgaaatgg atgaacaata tttctattac cgtttgggat atgcctatga
    25201 actttccgat aaaccaattg atgccataac tgcctatcgt aagggctatg aacaagaccc
    25261 ctattcacag gttgcctatc aaattgaaga ccgtatttta gagctaaaaa gcagtaaacc
    25321 gaatatagat attagtttcc tttaccctta ttcacctttg caagttacta ttgtgcaaga
    25381 agaacagccg gatacattaa agacaagtgt tactactgat tccaagtcct cctcttcatc
    25441 tactgcaaaa cagatagagg taaaacagga tactccggtt aaattaaaag aaaagcccaa
    25501 agaaggttac tggttgcaag caggaaggtt ttctgtagaa agcaatgcta atcgtttagt
    25561 aataaatatc cgtctgctca atataccggc tgcctattat gaagatgtaa gcggggggaa
    25621 aaaaagctgg gttgttgttt gcggttcttt tccagataga gctaaagcag aagaagcaaa
    25681 agaacttttg gcaacaaata atatcaattc ttttattacg gcatactaaa agtggacgat
    25741 aacaagctta ctccaatgct gaaacagtat tatgctgtga aagaaaagca tccggataag
    25801 ctcattcttt ttcggatggg tgatttttat gaaacatttt ttgaggatgc gcacacagca
    25861 tccagaattt tgaatataac tctcactacg cggaataaga atgacgaaaa tcctgttcct
    25921 ttagccggtt ttccctatca cgctttaaat acatatttag ataagctgat taaagctggg
    25981 ctgaaaattg ccatttgtga acaaacggaa gaccccaaaa aggcaattgg actggtaaag
    26041 cgtgaagtaa ctgaaattat aactcccggt gctgtattag accaatcctt tctggaaggt
    26101 aatgccaatg tttttttagc ttccttatat tataatgatc ctcagaagaa aattggctta
    26161 gcacatttgg atatttctac cggtgatttt ctttttacgg aactggataa agaagaactg
    26221 gtaaatgaat tacaaagatt tagggctgcc gaattaattg tagacagcac ccaagcggag
    26281 gaatttgtta aaaccctgcc tttagagacc ttgcctacga ttactgtttt tgacagctgg
    26341 cagtttcaac cccaagaagc aactgcaact ttaaaaaaac attttggagt taccacttta
    26401 gaaccctacg gagctcataa taaacttttg ggagccacag cagcaggtgc agcattagct
    26461 tatgtgcaag gactttacaa tactcccttt aatcacattt cttcattaag atattattcc
    26521 ctttcccaat atatgcagct ggatgaaatc agcagaagaa atctggaact ggttcgttca
    26581 atacgctatg gcacaaaata tggcagttta ctttcggtta tagaccaaac aatgacccca
    26641 atgggttcgc ggcttttaca gcaatggctg ttgcatcctc ttttagacat caaggaaatc
    26701 acttttaggc aggatatcat tcagtccttt attgataaaa gcagccattt aaaagaactg
    26761 cgtttaatct taaaggaaat tggagatatt acccgtttgg ttacccgttt gggctcttta
    26821 agaataaacc ccagagaact tattgctcta aagtcctatt tgtattccgc cagaaacctg
    26881 caaactaaac taagcacttt tgaacatccc caatttgctg tctggcaaca caatatgggt
    26941 agttttgagg atattatagc actcattgaa aaagctatta acgataatcc acctgtttct
    27001 ataaccgaag gcggaatttt tgccaagggc tataatcctg aactggatga gctgatggat
    27061 attatttatg atggtaaaag ctggatagct cgtttggaag aagatgaacg ccggaaaacg
    27121 ggtattaata atttgaaagt tggttataat cgtgtttttg gctattatat agaagtgagt
    27181 tctgccaaca aaaataaagt gccggattat tatattccca aacaaacatt gaccaatagt
    27241 gaacgcttta tttcaccacg cttaaaggaa tttgaggcaa aagtgctttc ttcggaagag
    27301 aagattaaga atttggaata tgaactgttt aaagaactgc gtcaaaactt agctgaatcc
    27361 ttgcctcgtt ttcaacaatt gagcgaagtt attgccgaat tggatgttct ttcttctttg
    27421 gcatttcttg cctggcagaa tcaatattcc cgtcccgttt ttaccgaaag ccgggaattg
    27481 catattatag atggcaggca tcccgttatt gagaaactga tggaaagcga taaattcatt
    27541 ccaaatgaca cacatctgga ttatccagaa accagtattg ctattattac aggacccaat
    27601 atggcaggaa aatcaaccta tttaagacaa gttggtttgc tggttatttt agctcaaatg
    27661 ggaagttttg taccggcaag taaaatgacc ctgcctgttt ttgatcgtgt ttttaccaga
    27721 gttggagctt ccgataatct ggctcaaggt caaagcactt ttttagtgga aatgatagaa
    27781 accgctaata ttctgcattc tgccacttct aattccctta ttttactgga tgaaattggc
    27841 agaggaactt ctacttttga tggcttatcc ttagcttggg ctattattga atatattcaa
    27901 aaatacaaac attctttaac cctttttgct actcattatc atgagctaac agaactggaa
    27961 aatctttatc cggatataaa aaattacaat gtagccgtaa agcaatggaa tgaagaaatg
    28021 atttttattc gtaaaattga acgcggtggg gcagaccaga gttacggaat acaggttgcc
    28081 cgtttagcag gaattcccga aaaagttatc cgtcgagcca aagaaatctt aaaaaacctg
    28141 gaagagcatg aaatcagtcc tcagggttta actgcaacca tccgtaagaa attagtgcgg
    28201 gatgttcctc agattgatat ttttgagatt ttagccgata aagccagtga aaacgatccc
    28261 ataataaatg aaatcaagga gattgatttg aataaacttt ctcctataga ggcatttcaa
    28321 tatctgcaaa agattcaaaa tcaactttta ggagaaaaat gaacaagctt cctacattta
    28381 ttgttattac cctgctgggt ttatttttcc ttgtttcctg cgctacaaat tcctcttccg
    28441 tggcaggaag cgtgaatgga acagagattt ctctggagga tttttttaat tcctaccgtg
    28501 ggcattatgc tatttttggt tatcagacag gacgcactcc cacaaaggaa gaaaaggaaa
    28561 aattgcataa tgagacctgg cgtgatatca ccaaagcaat aattctgcag gattattata
    28621 aaaagtataa catttctgcc agtgttgcag aagttctgga tacactttct aattccattc
    28681 ccgagcatat aatgaagtcc cctcgctttc aggttaatgg caaatttgac aaaaagttgt
    28741 atctgcaatc gctgatgacc gatcaaccgg aaaatttagc tgccttaaga aaacactatc
    28801 aggaaaatgt tattcctatt ctaaaactgc aaaaggtgct gatagaagag gaaatgctca
    28861 gttccaaaat ccgcaaacag gtagaacaga ttattgccag taatgcagat ttggagctct
    28921 atatttttgc tccctcccaa ttagaaattt ctttatccga tgcagaaatc agtgcttggt
    28981 atcaaactaa tttagagcaa tttcgtttat ggcagtttta tcgtttgggc tattgttcca
    29041 ttccggttat tccggacagc acagaactct ctgatgccaa aaatactgct gaatttattc
    29101 ggaatgaact tgcccgggga attgaaatta aggatattat cgctaatctg gaaaacaaaa
    29161 attctctggt aagttttatt gataacggct atcaaaaaac ggaagaatta ccttgggaag
    29221 ttcaaaatgc tctgtcagga ctggaaaatg gaaggtgcag tgaacccctg ccttatgaaa
    29281 agggctggat tatgtatcaa aaaatacaga gcaccaagac ccttactttg tataatacca
    29341 tttttgtgca ggcattgcct aaaacagcaa gtttattagc tccggaaaca caagcgcaac
    29401 aaattatgaa actggcatta aatataggcc ttcagcaagc agcagatgaa tttggcatta
    29461 gttatacttt aactcccgct atgagcccgg attccttaat tgttcctgcc aatgatatta
    29521 aaggaaaatt gatgaaatat ctggaaattg ctaatagcgg agatattata gagcccttat
    29581 attcagcaga gttatctgcc tggcttatct ttgaagtagt ggaaaaacaa agtaaagagt
    29641 atcttccctt ggaagaagta aaaccgcaaa tcgtgcaaat attaagtaag gaacgcagaa
    29701 aagaccaaaa taagcagatt gtagagcggt ggcttatcaa tcagaatata cctgcaaatt
    29761 atcaatggga aaaactggaa caagtaacca tagattccct ttggcaggga aaaccactta
    29821 ccaggatata ttatcaggca gttaaagcat atctggaaaa ggcaaatcct ccgcaaataa
    29881 cggagggtga ggttattata gttcccaaag taacagcttt tcgtccgcaa aaaacaaaaa
    29941 tcagtccgga acgcattaaa accatttata cgcaaaactt accggaggat tggtttaatt
    30001 cctggatgga ggagcaagtt aaaaaagcaa aggtagttat caatactaaa ccatgacttg
    30061 cttttcttcc caaaatcatt atctttttat gcaggaagca atagcggaag cgaaaaaagc
    30121 ttttgccgaa gatgaaatac ctgtgggtgc actgctggtt aaaaataaca ctataatatt
    30181 aaaggaacat aatcgtagcc agcagttagc caatccttta gcgcattgtg aaaaactcct
    30241 tattgacaaa attttaactt cagagcccgg gtttttatac gattatacgc tgtatgtaac
    30301 cttagaaccc tgtttgatgt gcgcaggaat gattatttta agtaaaatag gaaccgttgt
    30361 atatggagcc aaagatccga aaaccggtgt tgttggctcg ttgtataatg tgctgaatga
    30421 taaaagtttc aatcatcatc ctgtagttat tggcggaatt ctggaacaag aatgttcctt
    30481 cctactggag gaatttttcc gcaaaaaaag aactttataa aataaagagg agagttgccg
    30541 gagtggttga acgggacggt ctcgaaaacc gttgtacctt ttttacaggt acccagggtt
    30601 cgaatccctg actctccgcc agttgataaa ggaaatgaaa aatgatacat aaggattatt
    30661 tatgggctga tgccaatatt ccgcttattt cctgtgaccg cttaagagat gaacttctta
    30721 tttacaattt ggatgaatca gccacagcta atctgattga ccagtttgaa aaattgactg
    30781 gtaagacaat agataaattt tacaaaatag aggaactttc cggagggcag aaagttattt
    30841 tgatggcact tttggcaata tactctcctg cacctaaaat ccgttttgtg aatttgctga
    30901 atgcgcttga tcccaaacgc cgcgatgcaa ttcaaagctt gattcggaat agcggaaagg
    30961 atattattct ggacgaaagc ggatgattaa aattgcacca gggatagttt cttgctggca
    31021 ggattttagt ttgctgattg agcaggagct tttttttctg cctgaaaata tctattatct
    31081 acaaggtgaa aacggcagtg gcaaaagttc ttttataaaa cattccttgc ttcccgtttt
    31141 ggaaacccaa aggaatttgt tctattttct ctattttcag caactttttc atttgcaggg
    31201 ctatgctatt aagtcccatt ccgcatttta tcagccggaa ttgaaactga aaagcgaatg
    31261 ggattgtatt caatatctac tgcataatct ctccgaaata tatgctattg aatctaaacc
    31321 ggtttattgc attgtagatg aaaaccggta tttagcagaa gtatatcact atttgaaaga
    31381 gagtagtatc cctttttgtc tcattttttg tgaacattct tctttttcta ttgccgaaga
    31441 agttaacatc atcaattttc agttgcttac accaaatcag agtcgtgttt atgaaaccac
    31501 aatatagatt actgttggtc tctttagctc taattgtgtt ttttgttttc ttctgcctta
    31561 tttatctgga gaatatccct gtgcaattag ttgtcttggg agttgtatta ttgcttagtg
    31621 cttggacttt taagctaaag ggacttttaa agaaacttta tcacttcttg ccttttattt
    31681 tactgctttt cggggtttat tttatttttg ccctttttca gattgggcaa aacaaggatt
    31741 actggattca ttacggtatt acgcgaacta cattacttat cagcagttta atgtttatcc
    31801 aggtattaat cacttggctg aaaatagaca cctttctgga ttttcctttg ggaatagaaa
    31861 aattgaagta tattatttta ggtaaaatgc tttataaaat tgccttttct tcttattccg
    31921 agttatgcct ttttgtagat tccattcctg ctgagcaagc gagtaaaatt actctgaaaa
    31981 aaaagtttcg taaacgcctg attgttcttt tagcacttat tacttatgta attaacgagg
    32041 caacactgaa aggagaaatg attgatgaac gcatctggca ttgtcaccaa gttccgaagt
    32101 aattttgcct aaatagatga ataaaaagga gataaaatga aatggtatct tatagttgga
    32161 cttaccgtta tggttatgat cgctacggtt ctggtaagaa ttcctgttcc aggaggtggc
    32221 tattttaatt taggagatgt agttattatc tttagtggac tttttggtgg ctggaaagca
    32281 ggtttaattg caggaggaat tggttcggct cttgcagatt taatcggttt tcctgttttt
    32341 gcccctataa cgcttattgt taaagggctg gaaggattgg ttgccggatt ggcaaaaccc
    32401 gctataaaat ggcagattta ttttttcccg gcaatagcag ttataattat gattgccggt
    32461 tattttttgg gttgctggct ttttcctaat ctgggattgg cagtagctat agccgatttg
    32521 ccggtcaata ttattcaggc agttgcaggt tatattggag gtattgcctt acatcttgct
    32581 tacaccaaaa tttccagtac ttagcatttc cgagtgcatt attcaggttt atgtcttttt
    32641 tttgagttct gtaggaacga cagatattaa caacgggttt taacccggtg aaaaagaacc
    32701 caggatattc ttttcttcat cttccgaaaa aaagttccgt aggaacgaca gatattaa
//
LOCUS       DAOOZV010000015        31693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1575_length_31693_cov_60.889658, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000015 DAOOZV010000000
VERSION     DAOOZV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 31693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31693
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_31693_cov_60.889658"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..634)
                     /locus_tag="PLW08_02860"
     CDS             complement(185..634)
                     /locus_tag="PLW08_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPL51574.1"
                     /translation="MKKYLIIGLAVVLCLPAFLFCKEETNRLIGYWLTGDKTMKIEIY
                     DNGDSTFAGKIIWLKEPNDKNGKPKMDTKNPDEKLRTRPLLEMVILTGLIPESNTKYK
                     SGKLYDPNSGKTYSATIELVNNNTINIRYFIATPTLGRTDTWTRTSR"
     gene            complement(650..1945)
                     /gene="purB"
                     /locus_tag="PLW08_02865"
     CDS             complement(650..1945)
                     /gene="purB"
                     /locus_tag="PLW08_02865"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPL51575.1"
                     /translation="MIPRYSKPEMERIWTLENRYECWLEVELAAARAMFEKGIIPKED
                     WETINTKADFDTNRIDELEQITKHDVIAFLTNVGENIGPASRWLHFGMTSSDVLDTAT
                     GMQLKSSGELILTEISRLAEILKLKARQYKNTICMGRSHGIHAEPTSFGLKFALWYEE
                     MQRNIKRLNEAIEEISVGKFSGAVGNFAHLSPEIEELACKYLDLHPANVSTQIIQRDR
                     YAFFLSVLALIASGIEKIALEIRHLQRTEVHEVEENFSKGQKGSSAMPHKRNPIVSEQ
                     LCGLARILRSNALSAIENNALWHERDISHSSVERIILPDSCILIHYMLDRTCSLIENL
                     IVYPENMQKNLELTKGLVFSQALLLHLVQSGLTRENAYSFVQQEAMKSWETGSSFLDN
                     VLANKEITSVLAESEIRDIFSYERYLRNVDYIYRRCGIL"
     gene            complement(1942..2679)
                     /locus_tag="PLW08_02870"
     CDS             complement(1942..2679)
                     /locus_tag="PLW08_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51576.1"
                     /translation="MKKALISVLIISAIIISACAGPKDKSEEEKLRQQLKKWESFDSE
                     GIAEVSYKSLAIRKMFSAAKNGSELRFDLFDGGLLGSAGEPLLTMYLGDYVAVKSPFI
                     PMLELLDLTPSAPLQSLKLSANADSLVAFYGERIIRDKKLELNGVTINFNKDYLLDSV
                     WEQENKTQMKALYNSKGDLSELVVTSIDNISLRMSFDKIEYVQPQIIPLPKPELSFSE
                     DALKGLNNLNLESLLKDFLQYKKERQK"
     gene            complement(2825..>3316)
                     /gene="lgt"
                     /locus_tag="PLW08_02875"
     CDS             complement(2825..>3316)
                     /gene="lgt"
                     /locus_tag="PLW08_02875"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPL51577.1"
                     /translation="GLIYCHKHKLNFYALADAAIPIVAIGLGLGRLGNFINGELWGKP
                     TDLPWGMIFPGADNLPRHPTQLYELFLEGIVMFIVTYYLLKKLKKEGLVFWSFIGLYG
                     VFRFLIEFVREPDDLDFYTRFGYIFGFMSIGQFLSLIMIIVAIWGFYKLNAPHKKEKK
                     PKI"
     assembly_gap    3317..3416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3526..4311)
                     /locus_tag="PLW08_02880"
     CDS             complement(3526..4311)
                     /locus_tag="PLW08_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPL51578.1"
                     /translation="MHFIVCIKQVPGTTEIKIDPKTNTLIREGVESILNPFDAYAIEE
                     AVRLKEKYGGTVTAISMGPNQCETTLREAVSLGVDNIILLSDRRFAGADTYATSLTLA
                     AAIRKIGDYTLILTGQQAIDGDTGQVGPGIAAHLNIPQTCFVRKIESFNEQQAVVERL
                     LEDGFDRVSMQLPALISVVKEINVPRLPSLRGKRNAKTVPLTVWNCDDLGLDEKETGL
                     NGSPTQVLNIFSPHHEKQVEKFEGNSDEAVELIVQRLSELTNR"
     gene            complement(4325..5431)
                     /locus_tag="PLW08_02885"
     CDS             complement(4325..5431)
                     /locus_tag="PLW08_02885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPL51579.1"
                     /translation="MKVPMLDLQAQYKPLMPKIKEALERVFAEHNYIMGPQVKEFEEK
                     MANYLGIKNAIGCASGTDALVLAVKALGIGAGDEVITTPFSFFATASSIWRNNATPVF
                     VDIDPLTFNLDPGNIEKAITEKTKAILPVHLFGQCANMDSIMAIAHKYNLYVIEDNAQ
                     GIGCTWDGKMSCSFGDIGILSFFPSKNLGAMGDAGMCLTNNDDYAFKLRQLRVHGENP
                     KYYHQWVGLNSRLDTLQAAVLSVKLDFLEGWSKGRRANAEFYNEHLKNVPGIRVPYID
                     PKAVSIYNQYTLVCEKRDELMAYLKEKGIGCAVYYPLPLHLQECFSSLGYKKGDLPVA
                     EEMAEKVLSIPIYPELTREQKEYVCKTITNFYCH"
     gene            complement(5442..>6528)
                     /locus_tag="PLW08_02890"
     CDS             complement(5442..>6528)
                     /locus_tag="PLW08_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPL51580.1"
                     /translation="LENNQGLRRIRITRAGKIETYDLLKFYRLGDISQNPLLKDGDVV
                     FVPAIKDFISIGGGINLPGELEFVEGDKLGTIIELSLGFTFDADISKVQLYRYKENRI
                     DYDVLNYDLKANPAFWDLPLKADDRILISCDAEIRTRQRIKIYGQVKNPGEYVIDANT
                     TLYDVLQQAGGLTKRGDIKSMVYYNENINADPDPFLEMLMQRTMSEMTPLEYSYLRNN
                     LMQLKGKYSIDPVKMINSEGKEANPYLLDGDRIYIPEKIDMVWVSGQVKNPGMVPWVE
                     GKDWNYYIQAAGGYTNNRKTGKGRIIRANSGNWVKPGKNVVIRAGDTVFVPAQTDRSM
                     WTDVKDIVTLTSSVVTIILGIRTFTRD"
     assembly_gap    6529..6628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6629..6985)
                     /locus_tag="PLW08_02895"
     CDS             complement(<6629..6985)
                     /locus_tag="PLW08_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51581.1"
                     /translation="MKSRFLLILLMSLSLLSALTPTPTGDIIPISVSLTGFVENPGVY
                     QMTPVNRLSDLLLLNKTATLERIEKTQVLREKSVEVPKPAELLSPPTPEKEKEEIITL
                     ENNQGLRRIRITRAGKI"
     gene            complement(6975..7934)
                     /locus_tag="PLW08_02900"
     CDS             complement(6975..7934)
                     /locus_tag="PLW08_02900"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPL51582.1"
                     /translation="MKILITGGAGFIGSHLAERLLQEGNEVYVIDNLSTGRLENIESF
                     KDKPNFHLNIGSVLNRELLDKLISNVEQVYHLAAAVGVKYIIENPLLSLKTNIMGTDN
                     VLEFCNKYKAKALIASTSEIYGKNEQIPFSETDDRLLGSTHISRWGYGCSKAIDEFLA
                     LAYFREKKLPVVIVRCFNTVGPRQTGQYGMVLPKFIKAALLNQPLVIYGTGEQTRCFA
                     DVSDVVDAFIKLMNTPECAGEIFNVGTTESISITDLAQKVKDMCHSKSRIEYMRYEDA
                     FEEGFEDMMHRQPDLTKIKNFIGWEPKHKLDHIISRIIDYYEK"
     gene            8363..11257
                     /locus_tag="PLW08_02905"
     CDS             8363..11257
                     /locus_tag="PLW08_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPL51583.1"
                     /translation="MKKFFLLIFALLSLSILFAVPQYLIGLPVSELSLDKKDKTLRRA
                     VQDLAETDLEIYYYNDSYVIAGTDRNNYPGAINLGTPKSGKFYLITIGERYSKFDFST
                     VGEVLLEMGSVVLIKTDRDEIQLRQKIQNPFVPLLLEPMRFSETLSLQTAISSTRNEI
                     ENLINTVSEDSVLYFLQCLQNFSTRYALANNHLTVANWIKSQFQRFGINNSVLHSFQW
                     YGTMQYNVVATITGTVYPDTYIIVGGHHDSITNDNPMIFAPGADDNASGTVAALEMAR
                     VMMATNYQPKCSIRFVTFAAEEVGLCGSSNYANMADQTNMNIRVMINHDMIANTSPNF
                     WDPRVLLMPYDGFISHTDYAAMITSQYTALQPVYGYLNSSSSDSHSFWQHGFPVVYFF
                     EYNFSSVYHSNDDTVTNLDPVYCASVIRASTAVTASFANMPSPPANLTAQDEGTGNSI
                     HLSWNPVPDPDFSHFHISWGLSQNNLIYSQTTTNNEISITGLNTDQLYFFTVSSVDFS
                     GFESYSLATQCIPRLIPQTPVSFIDQPLPEIVKLSWQSNTELDLAGYKIYRSLNSSET
                     GALIATLPPTLCSYSDSNVQGGEFYYYYRLCAFDNDNNSSPFTPVLKTRPLSMNCGLL
                     IIDETADFSGSSPFQPTDEVVDNFYDLLIDGLWNTAHFDLADFTETLRLCDIGIYSVI
                     IWHGNDFSNMIYPNQVKDVLKNYIYYGGKVLFSVFHPGLAFELNADYPTVFSADSFIN
                     EVLGISGTDYTIQARFNKAESVYSGYPDINVDPNKVPASFNGHLYQVEALTANAQATP
                     IYHYASAYDNNSPQGILNGGVVGVQNTYFSGKTVTFSFPLYFMDFNQSRQVVHYIIEN
                     YFGFPVAVGDEVNIPVAEISILPNQPNPFSVSTQINILCKNSNAPLQVQVFNLKGQLI
                     KTLFQGKPEGKNTLSWDGNDNNGKKVSSGIYFLKAQQGNKVQTRKILLLK"
     gene            11638..13194
                     /locus_tag="PLW08_02910"
     CDS             11638..13194
                     /locus_tag="PLW08_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL51584.1"
                     /translation="MSKKLENNSTSIGRIINLLYRYLMVASFITIIAVDSHSELNANN
                     IVHEPNSQLNVFQEDFNYFYRILLDSHPGIETDNTSFNQKVEAFMECLATVTDTLQFE
                     ARLRAFTHDLHDGHTRVSSFSNQGSPIYPIGLWWHESGWYVEMISTEFKDLIGSKVIS
                     INGIDAETFMTKLMQLTTGENVYWLRDQVSFFLRNSANLHALNLDRPDGVLQLIIEQN
                     GTFSEVNLYEVERPSLWGNQKTGITALHDDNYWGQALPEDNVYYIQFNAMSDPVGTSD
                     TPFSGWISFLKESFALIDSLGIDNLVIDLRNNSGGNSTMGDILLSFCSLPDSIKYFGG
                     KMKISDLVLNHYQMDLTSMNKALSSDLGYEVLETELPFTVIFYDGKSPYLRPRTLGEI
                     VPNVMSNDSIKKYNGKLILLTGSQTYSSAIMFATLCQDNGLAIVYGSPTGGKPSSYGE
                     GLNFRLPNTGIMCSVSVKYFQRPDTSRDPSDSLYPDVDVLITPEEYFSPIDTLWERVL
                     NDIRNSNLYK"
     gene            complement(13240..13497)
                     /locus_tag="PLW08_02915"
     CDS             complement(13240..13497)
                     /locus_tag="PLW08_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51585.1"
                     /translation="MNIELEKYPVENNHECFIRAVTNKLVLMYYGSYEAEVILKWDEE
                     QGFVYVRQFIKALDQYEMARVKGDVNIKQYFPALLRYFPKI"
     gene            complement(13865..14095)
                     /locus_tag="PLW08_02920"
     CDS             complement(13865..14095)
                     /locus_tag="PLW08_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HPL51586.1"
                     /translation="MPYKEYHLTKNKQGWKVKKANAQKAVKVFSTKYSALQYARKLAN
                     NQKAELVIHSSDGKIQDKRSFGNDPCPPKDKR"
     gene            complement(14085..14645)
                     /locus_tag="PLW08_02925"
     CDS             complement(14085..14645)
                     /locus_tag="PLW08_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51587.1"
                     /translation="MILILDNTVLLNCIDRCSSVDLIQKLSEYPLEMEIVETVHNEYI
                     KGSKIYPDRCNLTLFNKHLYTTIKVIDDSKLTINRVPQLINLDGGELFSVVYLLENKT
                     DRILCTDDKATHKVLAKFNIKCLWTINLLQLLYQRKPALLTYNQVWDCYKEMIAKGFY
                     GLPTKNIDINAYINIKNYLSGGQNAL"
     gene            complement(14647..15717)
                     /locus_tag="PLW08_02930"
     CDS             complement(14647..15717)
                     /locus_tag="PLW08_02930"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPL51588.1"
                     /translation="MNVGEKIKKYRKRLNISGKELAQKLGVTPALISYYEQGKRQVPL
                     KTLMQISEIIKVPLEYLVTDKEYIAYSAYRCKGRITKEEKEEVALFQEIVDNLIYVAD
                     INNFNLVYHGPDEFRSKTISDQQIGIIKANLELPVITDYNSLVEALWLKWRVVVFALP
                     FKNKNLSAITIKRDGVYCVFINKGHTLERNFFSLAHELGHILMHLETDEFIISRISSR
                     DLKEKEANDFASRFTVSYALLIDNVNERSSSEMITAEQIRELAKYFNVSYECIAYNLV
                     KANILNYGKDEIARLKEPIKEYEYKIAINDFPAIYHLLVYFALAKGEISISKAAKYLL
                     SDIQSVNDEFRKIGKILGKGYS"
     gene            complement(16190..16921)
                     /locus_tag="PLW08_02935"
     CDS             complement(16190..16921)
                     /locus_tag="PLW08_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HPL51589.1"
                     /translation="MIDENDLNEYVENHIEIFHNNRIMNISNLKLANILKHKNPYLFR
                     AKNILNAGELIKTILDAHLSSAEETMFGEFLESLAIYICGMIYGGVKSSSPGIDLEFN
                     KENKRYIVSIKSGPNWGNSRQVAKMIDDFNRTSRIIRSNNKETTVIAINGCCYGKDDK
                     PDKGSYFKLCGQRFWEFISCDEEMYTKIIVPLGYKAKEKNDNFYAEYSKLINNFTAKF
                     IQDFCDKGVINWNKLLQFNSGKKVC"
     gene            complement(16934..17761)
                     /locus_tag="PLW08_02940"
     CDS             complement(16934..17761)
                     /locus_tag="PLW08_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51590.1"
                     /translation="MNNNEYELYLGDALEILQTIEKESIDLIVTSPPYADSRTNTYGG
                     IKPDEYNEWFLPITQELLRILKPTGTFILNIKEKVVNGERHTYVIELILNMRKQGWLW
                     TEEFIWHKKNCYPGKWPNRFRDAWERLLQFNKNKFFKMYQEEVMIPIGDWAEKRLSNL
                     SQTDKIRDTSKVGSGFGKNVSNWVGKDMVYPTNVLHLATECGNKNHSATFPYALPEWF
                     IKLFTRPGDVVLDPFMGSGTAVFAALNMGRKAIGIDINPEYYNLVLGKIQNQQQVLF"
     gene            complement(18124..19122)
                     /gene="argF"
                     /locus_tag="PLW08_02945"
     CDS             complement(18124..19122)
                     /gene="argF"
                     /locus_tag="PLW08_02945"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPL51591.1"
                     /translation="MSFNLKNRNFLTLMDFTPREIQFLLDLAIQLKKAKYAGTEQQKL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHTTYLGPTGSQLGKKESVADTARVLGRMY
                     DGIEYRGFGQEIVEELAKYAGVPVWNGLTDQDHPTQVLADFLTALEHLNKPLNEMVFV
                     YCGDGRNNMANALMIGACKTGMDFRIVSPKTLFPEQNLMHKCLKVAQETGAKITLTDN
                     IDAGVKDADVLYTDVWVSMGEPDSVWEQRINLLKPYQVNGEMLKKTGKSTTIFMHCLP
                     SFHDLKTKIGKDIYEKFGLTAMEVTDEVFEGPHSVVFDEAENRMHTIKAVMVATLG"
     gene            19578..20024
                     /locus_tag="PLW08_02950"
     CDS             19578..20024
                     /locus_tag="PLW08_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51592.1"
                     /translation="MKVNFQYGLAGYTGKADGLVFCFDRKSGIVYARKRVYPRLTEEN
                     ARIGNISHNIFSLKPSEEYKDDLRLYLMGYNGLRANSHKPLRSWVNLYHRLMANMAKK
                     NPEIDLKTLTRQQIFADNLPCKSIKSAVEAGILPPINGYERMTALI"
     gene            20310..21554
                     /locus_tag="PLW08_02955"
     CDS             20310..21554
                     /locus_tag="PLW08_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPL51593.1"
                     /translation="MKVITVILMLLPLLLAAQSYTLEELITYGLENSFSVQKSALSYQ
                     SAKSTLNTSKWNLLPDASVSGGIKKDFDLAVNTDDVTSSAGLSIGKTISLNDAAYFNY
                     LYASLDTKTANMKLEQTRRAYAYEVFSAYLKVLSSQKQRSSLEENLQIQIRVWEQSKI
                     FLQLGKTTPFEVKQNEIAVLNSNISLMKLDNTIANARKELFALVQMQDEDYPLTELEL
                     NIQKEIPAFNTEKNADLNILNQELKRLNLQLKQNKLDDFPRLNLAYNFDRSVSGADFD
                     FDHYNTNHGLSLTLSYPLFNIGENAESRTRTKISRQLSQVAIEEKKDQLQRDYDSALR
                     ELQYLKRMNELYSEQLAQSREQITQAEERYRLGLIELLELDKTRTNYIETDISYNTNL
                     YSIIAKQEEINYLLSEPVLGKW"
     gene            21591..22886
                     /locus_tag="PLW08_02960"
     CDS             21591..22886
                     /locus_tag="PLW08_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL51594.1"
                     /translation="MKKYLKYIIIIALILIAIIVIISLRKAKNKPEFRTTSPSFGNIR
                     EVVTATGSLNPYVLVSVGTEVSGKIDKLYKDFNDKVKKGELLAKLDTEILATNLEAAK
                     ADLAKAKTALDQAKLDYDLQAELLEKKMTPEYDAKKALFAYQTAQQNFNNAQLTLQRA
                     QKNLANAYITSPINGIVVSRDVDEGQTVAASMSSPTLFKIANNLDQMQISANVDEADI
                     GKIKVGLPVEFSVDAYPSENFSGNVKQIRLNPTTESNVVSYSVIIDAANPEQKLLPGM
                     TTNVTIIIQARENVMRISETATRFKPSKEIWEQLGYKWSDDLLLKAQKEAYASLAKAK
                     TIPAKTDSTNFNQKKYGKTKQEFSSKTISSSGKAALIWVLEDNQPKPKGIRTGISESG
                     YIEVIEGLTGNETIITGVNTKSGSETNNGTQGGPGMRHF"
     gene            22888..23640
                     /locus_tag="PLW08_02965"
     CDS             22888..23640
                     /locus_tag="PLW08_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51595.1"
                     /translation="MPNLLIRTENLTKIYQMGEVQVHALRGVSLQIKEGEFVAIMGAS
                     GSGKTTLMNLLGCLDLPTSGEYYINDIGVHEITPAELTKIRNQMIGYVFQNFYLLPRT
                     TALENVELPLLYRNKMTHHQRLQKAMEALSTVNIEDRAKHYPNQLSGGQQQRVAIARA
                     IVNDPVFLLADEPTGNLDTRTSLEVMTIFQELHKNGKTILLVTHEHDIAMYAHRLITF
                     RDGRIIADVPNSNPHDAAYDLANMPKLPGDEE"
     gene            23637..24854
                     /locus_tag="PLW08_02970"
     CDS             23637..24854
                     /locus_tag="PLW08_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51596.1"
                     /translation="MSIVGIIRIAFKSLARNKTRTFLTMLGIIIGVAAVITMLAIGQG
                     AKKIVEDQISSMGTNVLMVTVNFTRSSTNVRQAAGSGSLLELDDVFAIRSQVEDALYV
                     SPVYNSFAQLKFESQNWRTGVMGVDTDYFYIRNMNPEQGELFYDSDVENGTKVCVIGK
                     TVAENLFGDIDPVGQIIRIRNIPFTVKGVMESKGQNVMGADQDDSVWAPYTTVYTRLL
                     GRRWRNMMIIVSAVSKDKIAEVQQDILDLLQSRHRGTTSEEFLVRSQTDIAETASKVS
                     DTMTFLLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGAGKRDVLLQFIIEA
                     VTISILGGFIGILLGFGASKIVGNAMKWSVVVTPFSVLLSVGFSMAIGIFFGWYPASK
                     AANLNLIDALRYE"
     gene            25008..25178
                     /locus_tag="PLW08_02975"
     CDS             25008..25178
                     /locus_tag="PLW08_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL51597.1"
                     /translation="MAVKIDKETCIGCGACIDVCPVSALKLVNDKAECDESLCIDCGA
                     CIDTCPVQAISQ"
     gene            25343..27907
                     /locus_tag="PLW08_02980"
     CDS             25343..27907
                     /locus_tag="PLW08_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51598.1"
                     /translation="MKRLFLILFITTLISLNFAIQVSGNQSGVWSPVNNPYEVIGAIT
                     VPASDSLIIQAGVEIHLLGSYQITVEGILNAYGTEVDSIRILNMQANPTALWPGLRFE
                     NLQHTSHLNYVYLEYATYGVRTINSPIAISHCHINLCEKGMEFYGIGSAVPLPNIVEY
                     CLIENCTQNAILVTSNSNAYIRYNEIRYNGTGAQFRAAIQMGNQSGVNQCNPNILYNH
                     IHHNYKQGISAWDIASSGSINPQILHNIIEYNYTGIYLLQASGYVADNQINYNFIPGD
                     MNSGAGVMVSGVTSVPYFERNHIEGNYTGFYITNNGKPVLGDLTLNHIWAQGENTIIN
                     NIDVNNHNNSVYCDAYPNANFIIMAENNNWGFATAAEIALTINDHNDNSALPTVDFEP
                     FIIPLQNTTITGSYIYTGTAQIANARLELIAVTNGIVQVSIPLTTADFSVQANISEDF
                     YAQVVLTETGTGKELYGCAGSYINPGVFSPAPGNSVDIGIVEVTDIPLSRYEYVGEPI
                     QEGNLTLYPLLTGWGLYSYDKLDWVYPAGDYLYLKRNIWRTPAGEVTTELPLGTVYKK
                     YLNINAGDSWQQTEVIYPGNITFTTDVQVTNCVTDISTPAFLLFTRKDSFGYVVDKRI
                     KGIAEELFHYQNHYNIARENIIYLPGNNDPLTTGNVTLFMPEPLSESPSFLAFEPDID
                     DQIHLFWQAPAQGNFSWTSYRIYKNDALIGEIPFSQSDYIVTDFNPQITTYFYVTATD
                     GINESEPSNCVTVYYVGNEDLIQKPFSVDVYPNPVSFTSGSGLIVEVKNAENSSALIE
                     IYNLKGQLVFRKNLQDEKNFLWKGIDNRGKRSSAGIYFLKIKVQGEKPVTRKIMVL"
     assembly_gap    28152..28251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28252..31046)
                     /locus_tag="PLW08_02985"
     CDS             complement(<28252..31046)
                     /locus_tag="PLW08_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPL51599.1"
                     /translation="MKRTYLGLILLCCILTLGAILPPEYASFPLEQDPDVISGTLDNG
                     LKYYIKQNAKPEKRLELRLLINAGSVVEDDDQLGLAHFVEHMAFNGTKNFPRTEMVDY
                     LTSIGMGYHNGLNGGTSYDFTVYEFKLPTDDEAKMRKGISILSDIAWQVSFEPAEIER
                     ERGVVQEEWRLGQNAQRRIQDQIDKVRFAGSRYAERNPIGTIENLKNFKHESLIRYYQ
                     DWYRPDLETVFIVGDYDPQKLEGLVKEYFGVIPKRENPRPRLSYPVPDNIEPRAVTVL
                     DKEQPYTMIRSTWKVKATPVTDLGSLYNEMKQDLFFTMINARLEELSQQPDPSFSYAF
                     MFNATWLKGFNATDCFMLANEGRSEDAFRTLITELTRVRQHGFQPGEWERAKQIMIRQ
                     AEKWVADKPTMDSEDVIWELLDAVMSEDTILSAETYEQMLKGLIYEIALSEVNDIVDD
                     VITSENLTLSLAGTDKPGAKYPSREDLLNIYQQSIAQELEPWEDITVNEPLLETIPIP
                     GKITKEKVFPKSGIKQWVLSNGITVYSKKTDFKADEVILIAQSPGGKAKLKPENYKSA
                     DLLSQYFSVSGFGNFDAPALQKALAGKIVYVYPTIYSYSEGWRGSCSPQDLELMFQML
                     YQYNYAPRYNEEAFSAAVASTRARVQNYLLEPSNAFFDTLNVLMFNNHPLKRNLHPED
                     LDSVTLKQLKDIFQDRFGDYTDFTFYVVGNFAEDQLKDYCQTYLANLPAKGRKEKMTD
                     AGVRAFNGKKEISFHKGTERSFVSNVTNNKASFSPQNNVQQSALQMIAYEKLRENVRE
                     NMSGAYVVEIQSFYDFLPKPGVFTLTLMGCDPERAKELNAAIFATLDSLKNGLFADKY
                     IESTKTTLHKKYEENIKSNRYWVNNMSENISYGLPIDCFLDYPVLYDKLDKKAITKTA
                     KQYYVFDKSLLSVYMFP"
     gene            complement(31095..>31693)
                     /locus_tag="PLW08_02990"
     CDS             complement(31095..>31693)
                     /locus_tag="PLW08_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51600.1"
                     /translation="CLILGLAAILLMLSACESGGKLQVFNRCSYPAYIKVENMEQKTI
                     PAGESVSFNIDTDTQNIFTGEVKKKLPIWLVGETFSIFDEYQNAYLDSTWIEVKAGKT
                     YSIYLNPNRASVKVINNSDQIISWAEIWQHSPLTYYQVGVMYNILPGESRFLRVDYGN
                     KYYYQVEIYLPDESHYTFCDSENILAVDEQYVVNFNPN"
BASE COUNT         9717 a         6209 c         5599 g         9868 t
ORIGIN      
        1 tactatcata atggaaaata tataatagct ttccttgttc cccaagtgca gacccagaac
       61 atcacggtaa ccatattctt tgagaccaat cgctattaac accacagtaa aattaacttt
      121 accctctgtt ctgatcttgg tgtagatcgc atccagccag ataattactt atcctgctta
      181 aaaattagcg ggaagtgcga gtccaggtat cggttctgcc taaagtaggt gtagcaatga
      241 agtaacggat attaatagtg ttattattaa ctaattcaat agtggcggaa tatgtttttc
      301 cgctattggg gtcataaagt ttaccagatt tatatttggt attgctttcg ggaatgagtc
      361 cagttaaaat aaccatttcc agcaaaggtc tcgtccgcag tttttcatcc ggatttttgg
      421 tgtccatttt aggtttgccg tttttgtcgt tgggctcttt cagccaaata atcttaccgg
      481 caaaagtgct gtctccattg tcgtaaatct ctattttcat tgttttatca ccggttagcc
      541 agtagccgat aaggcggttt gtttcctctt tgcaaaaaag gaaggcagga aggcataaaa
      601 ccacagccag cccgataatt aagtatttct tcattttttt gctcccgggt tataaaattc
      661 cgcaacgcct gtaaatataa tccacattcc gaagatagcg ttcataagaa aatatatccc
      721 gaatttcact ttccgcaagg acagaagtta tttccttatt tgccaaaaca ttatccaaaa
      781 aactactgcc tgtttcccag gatttcattg cttcctgctg aacaaaggaa taggcatttt
      841 cccgggttaa accgctttgg acaagatgca acagcaatgc ttgcgaaaaa acaagacctt
      901 tagtcagttc caggtttttt tgcatatttt cgggataaac tatcagattt tctattaggg
      961 aacaggttct atccagcata tagtgaatta aaatgcaaga atcaggcaaa atgatgcgct
     1021 ccacactgga atgagaaata tcgcgttcat gccacaaggc attattttct atagcgctta
     1081 aggcattgga acgcaaaatt cttgccaaac cgcaaagctg ttcactaaca ataggattgc
     1141 gcttatgggg cattgcagaa gaaccctttt gccctttgga aaagttctct tccacttcgt
     1201 gcacttcggt tctttgtaaa tgccttattt ccaaagcgat tttttctatg ccggaagcaa
     1261 ttagtgctaa aaccgaaagg aaaaaggcat atctatccct ttgtataatt tgcgtggaaa
     1321 catttgccgg atgcaaatcc agatatttac aggcaagttc ttcaatttcc gggcttaaat
     1381 gtgcaaaatt gcctaccgca ccggaaaatt taccgacgct aatttcttct atagcttcat
     1441 ttaagcgttt tatattccgc tgcatttctt cataccaaag ggcaaacttt aagccgaaag
     1501 atgtaggttc tgcgtgaatt ccatgagaac gacccataca aattgtgttt ttatactgcc
     1561 tggctttcaa tttcagaatt tccgccaacc gacttatttc cgttaaaata agctctccac
     1621 tgctttttaa ttgcatccct gtagcagtat ccaaaacatc ggaagaggtc attccaaaat
     1681 gcagccaccg ggaggcagga cctatatttt cccctacatt ggttaaaaaa gcaattacat
     1741 cgtgcttggt aatctgctcc agttcgtcaa tgcggttcgt atcaaaatcg gctttagtgt
     1801 taattgtctc ccaatcctct ttaggaataa tgcctttttc aaacattgct ctggcggctg
     1861 caagctccac ttccagccag cattcgtaac ggttttccaa agtccagatt ctttccattt
     1921 caggtttaga ataacgcgga atcatttttg cctctccttt ttatattgca gaaagtcctt
     1981 cagcagtgat tccagattta agttgtttaa tcctttcaag gcatcttccg aaaaggaaag
     2041 ctcaggtttc ggcagaggaa taatctgggg ctgaacatat tcaattttat caaaactcat
     2101 tctaagagaa atgttgtcta tactggtaac aactaattcg gaaaggtctc ctttactatt
     2161 gtataatgcc ttcatttggg tcttgttttc ttgttcccaa acactgtcta aaaggtagtc
     2221 cttgttaaaa ttaatagtaa ctccattcag ctccagtttc ttatcccgaa taatcctctc
     2281 gccgtaaaaa gcaacaaggg aatcggcatt agcagataat tttaagctct gtaaaggagc
     2341 ggaaggtgtt aaatctaaaa gttccagcat cggtataaaa ggtgatttca cggcaacata
     2401 atccccaaga tacatagtaa gtaagggttc tcctgccgaa cctaaaagcc cgccatcaaa
     2461 aaggtcaaag cgtaattcac taccgttttt ggcagcacta aacattttac ggatagccaa
     2521 acttttatac gagacctctg ctatgccttc actatcaaaa ctttcccatt ttttcagctg
     2581 ctggcgtaat ttttcttctt cgcttttatc ttttggtcct gcacaggcag aaatgataat
     2641 agcggagata attagaacgg atattaatgc tttcttcatt attaacctca atcgttttct
     2701 cggtttgccg aatttctctt gtaaagggac ttatgttatt attggcaaga aaagtccttt
     2761 gtcattcccg caaaagcagg aatccaaatc aaaataacca tttctcatat acaggcaaac
     2821 taacttatat tttcggtttc ttttcctttt tatgcggagc attgagtttg taaaaacccc
     2881 agatagccac aataatcatt attaagctta agaattgacc aatgctcata aagccgaaaa
     2941 tgtaaccaaa acgggtgtaa aaatcaagat catccggctc acgcacgaat tcaattagaa
     3001 acctgaaaac accatagaga ccaataaaac tccaaaaaac cagcccttct tttttcagct
     3061 ttttcaacag ataataggtg acaatgaaca ttacaatgcc ttccagaaaa agttcataga
     3121 gctgagtagg atggcgcggt aaattatctg ccccgggaaa aatcattccc caaggtaaat
     3181 ctgtcggttt tccccataat tcaccattga taaaattgcc taagcgtccc aatcctaaac
     3241 ctatagctac aataggaata gcggcatcag ccagagcgta aaaattcaat ttatgcttat
     3301 gacaataaat cagcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg
     3421 caagaataaa actcagcaca taaaaaaagc cataccagcg aatatgcaga tttagccctc
     3481 ctaatttaaa actgacgata tcggggctga tttcaggata tttaatcatc tattagtaag
     3541 ctctgaaagg cgttgcacaa ttagttccac tgcctcatca ctgtttcctt caaacttttc
     3601 cacctgcttc tcgtgatggg gagaaaaaat attcagcacc tgtgttggag aaccgtttag
     3661 tcctgtttct ttttcgtcca gtcccaaatc atcacaattc caaacagtta gcggaactgt
     3721 tttggcattt cgttttcctc ttaaagaagg taaacgggga acattaattt ctttcaccac
     3781 ggaaattagc gccggaagtt gcattgaaac acggtcaaaa ccatcttcca gcaagcgttc
     3841 cactactgcc tgttgttcat taaaggattc aattttgcgg acaaaacagg tctgcggaat
     3901 attcaaatga gctgcaatgc caggtcctac ctgaccagta tctccatcaa ttgcctgttg
     3961 ccctgttaaa ataagagtat aatcccctat ttttctgata gcggcagcta aagttaaact
     4021 tgtggcataa gtatcggctc cagcaaaacg cctatctgat agtaaaataa tattatccac
     4081 acctaaggaa actgcctcgc gtaatgttgt ttcacattga ttgggaccca tactgatggc
     4141 agtaacggtt ccgccatatt tttcttttaa gcggactgct tcttctatgg cataagcatc
     4201 aaaggggttc agaatacttt ccaccccttc ccggattaag gtattggttt tagggtcaat
     4261 tttgatttcc gtagttccgg gaacttgttt tatacagaca ataaaatgca tatttctatt
     4321 tccttcaatg gcaataaaag ttggttatgg ttttacatac atattctttc tgttcccttg
     4381 taagctcagg ataaattgga atggaaagaa ctttttcagc catttcttcc gctaccggta
     4441 aatcaccttt tttatagcca agcgaactga aacattcctg caaatgtaaa ggtagaggat
     4501 aatagacggc acaaccgatt cctttttctt ttaagtatgc catcagttca tctctttttt
     4561 cgcagacaag cgtatattga ttgtaaatgc ttactgcctt cgggtctata tagggaacac
     4621 gtattccggg aacatttttt aagtgttcat tgtaaaattc agcattggcg cgtctgcctt
     4681 tactccaacc ctccagaaag tccagtttaa cgcttaaaac agctgcttgc aaggtatcca
     4741 aacgactgtt caaaccaacc cattgatgat agtatttggg attttcacca tggactctca
     4801 attgacgcaa tttaaatgca tagtcatcat tattggttaa acacattcca gcatcaccca
     4861 tagcgccaag atttttagac gggaaaaagg atagaattcc tatatctcca aaagaacagc
     4921 tcatttttcc gtcccaggtg caaccgatac cctgtgcgtt atcttcaata acatacaaat
     4981 tgtatttatg agcaatagcc ataatggaat ccatattggc acattgacca aaaagatgca
     5041 ccggcaaaat tgccttggtc ttttcggtta ttgccttttc aatattaccg ggatcaagat
     5101 tgaaagtgag agggtctata tccacaaaaa caggagtagc attattgcgc cagatagaag
     5161 aagcagtggc aaaaaaactg aaaggagtag taataacttc atcacctgct ccaattccca
     5221 atgctttaac ggctaaaacg agagcatctg ttccggaagc acaaccgatg gcatttttaa
     5281 tcccgaggta atttgccatc ttctcttcaa attctttcac ctgaggtccc ataatatagt
     5341 tatgttcagc aaaaaccctt tccagtgctt ctttaatttt aggcatcaga ggtttgtatt
     5401 gtgcttgcaa atccagcatc ggaactttca ttttttctcc tttagtccct ggtaaaagtt
     5461 ctaataccaa ggatgatggt aactacagaa gaagtaagcg ttacaatatc ttttacatct
     5521 gtccacatag acctgtctgt ttgagcagga acaaaaacag tatcacctgc tctgataact
     5581 acatttttcc cgggcttaac ccaatttcca ctgttggcac gaataattct gccctttccc
     5641 gttttccgat tattagtata gccaccggct gcctgaatat aataattcca gtcctttcct
     5701 tccacccagg gaaccatacc gggattttta acctgaccgc taacccaaac catatcaatt
     5761 ttctcaggaa tataaatacg atcgccatca agcagataag gatttgcttc ttttccttcg
     5821 ctatttatca ttttaacggg gtctatactg tatttgcctt ttagttgcat taaattattg
     5881 cgtaaatagg aatattccaa aggggtcatt tcactcatag ttctctgcat cagcatttcc
     5941 agaaaggggt ctgggtcggc attgatattt tcattgtaat agaccattga ttttatatca
     6001 ccgcgtttgg ttaagccacc tgcctgttgc agaacatcat ataaagtggt attggcatca
     6061 attacatatt caccagggtt tttaacctga ccgtagattt taattctttg cctggttcta
     6121 atttccgcat cgcaggaaat aagaattcgg tcatccgctt ttaagggtaa atcccaaaat
     6181 gcaggattgg ctttcagatc ataatttaaa acatcataat caattctatt ttctttatag
     6241 cgatagagct ggactttgga aatatcggca tcaaaagtaa aaccgagaga taactcaatt
     6301 attgtgccaa gtttatcacc ttccacaaat tccagttcgc caggcagatt tatgcctcca
     6361 ccaattgaaa taaaatcctt gatggctgga acaaaaacga catcgccatc tttcagcaaa
     6421 ggattctgac tgatatcacc caagcggtaa aacttcaaca aatcgtaggt ctctattttc
     6481 ccggcacgag ttattctaat tctgcgcaga ccctgattat tctccaaann nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ttttcccggc acgagttatt ctaattctgc
     6661 gcagaccctg attattctcc aaagttatta tttcttcctt ttctttttca ggagttggag
     6721 gactcaataa ttcagctggt ttaggaactt caacactttt ttcccgtaaa acctgtgttt
     6781 tttcaatgcg ctccagcgta gctgttttat taagcaataa aaggtcggaa agacggttta
     6841 caggagtcat ttgatagacc ccggggtttt ccacaaaacc cgttaagctc acagaaatag
     6901 ggattatatc tcccgttggg gtaggtgtta gagccgaaag caagctcaag ctcattaaaa
     6961 gaatcagcaa aaacctactt ttcatagtaa tcaattatcc tacttatgat atggtccaat
     7021 ttatgttttg gctcccagcc aataaaattt ttaatttttg ttaaatccgg ttgcctatgc
     7081 atcatatctt caaaaccttc ttcaaaagca tcctcatatc tcatatattc aatacgggat
     7141 ttgctgtggc acatatcttt aaccttttgc gctaaatcag ttatagaaat ggattccgtt
     7201 gttcctacat taaaaatctc accggcacat tcaggagtgt tcatcagctt tataaaggca
     7261 tccactacat ctgaaacatc agcaaaacaa cgcgtttgtt ctcctgtgcc ataaatcacc
     7321 agcggttgat ttaaaagagc ggctttaata aatttgggca aaaccatccc atattgtcct
     7381 gtctgcctag gacctacggt attaaaacaa cgcacaatta ctaccggcaa cttcttttcc
     7441 cgaaaataag ctaatgccag aaattcatct atggctttac tgcaaccata accccaacga
     7501 ctaatatgag ttgaacccag taatctgtca tctgtttccg aaaagggtat ctgttcattt
     7561 ttaccgtaaa tttcactggt ggaagcgata agtgctttgg ctttatactt attacagaat
     7621 tccaacacat tatctgtccc cataatattt gttttaagcg ataaaagagg attttcaata
     7681 atatatttaa ccccaaccgc agcagctaaa tgatacactt gttctacatt actaatcagt
     7741 ttatccaaaa gttcacgatt cagcacactg ccaatattta agtgaaaatt gggtttatct
     7801 ttaaaggatt ctatgttttc cagacggcct gtggaaaggt tatctatcac ataaacttcg
     7861 tttccttcct gcaatagcct ttccgctaaa tgggaaccga taaatccggc tccgcctgtg
     7921 atcagaattt tcatattttc tccattctgt aaaagcccat tataacacca ataatgaatt
     7981 atcaggcata aggttttcag ctaagaattt tgtcaatgag aaagtgggaa tttcacaaag
     8041 agagaaaaat tagacaccga aaacattgta aacaccaaga tttttttacc aagagaaaaa
     8101 gagaaagatg tgatagcaaa atagttatat cgtttttgaa tgataagcta tttcccattc
     8161 ctgtgaaaac agaaatccag acatttttaa cctatctgac tgaattttca ttatgtaact
     8221 tatatcagta tgtcccaaat aaggcaggaa tgtaaaaatt ataaattatt tcggtgtttt
     8281 cggtgtcctc ggtgtaaaaa aaccattaac aggaatgtta tttgcttttt tatcgtatat
     8341 agatatccaa aaggagatac agatgaagaa atttttcctt ctaatttttg ccctgctatc
     8401 tttaagtatt ttatttgctg tcccccaata tctaatcggt ttgcccgtat cggaactttc
     8461 tttagataag aaagataaaa cactgcgcag agcagttcaa gaccttgctg agaccgattt
     8521 ggaaatatat tattacaatg attcttatgt tattgctgga acagacagga ataattaccc
     8581 cggagccata aatctgggaa caccaaaaag cggaaagttc tatctaataa cgattggtga
     8641 aagatactcc aagtttgatt tttcaaccgt aggagaggtc ttattggaaa tgggttcggt
     8701 agtgttaata aaaacggata gagatgaaat tcagcttcgg cagaagattc aaaatccttt
     8761 tgtcccttta cttctggagc caatgcgttt ttctgaaact ctttctttgc aaacagcaat
     8821 tagttctacg cggaatgaaa tagaaaattt gattaatact gtttcggaag atagcgtttt
     8881 atatttcctt cagtgcttgc agaatttttc tacgcgttat gctttagcta ataaccattt
     8941 gacagttgcc aactggatta aatcccaatt tcagcgtttt ggcattaaca attccgtttt
     9001 gcatagtttt cagtggtatg gaactatgca atataatgtt gtagctacaa ttacgggaac
     9061 tgtctatccc gatacctata ttattgtagg tggacatcat gattccatca ctaacgataa
     9121 tccaatgatt tttgctcccg gagcggatga taatgccagt ggcacagttg ctgccttaga
     9181 aatggcaaga gtgatgatgg caactaatta tcagcccaaa tgttctattc gttttgtaac
     9241 ttttgctgca gaagaggttg ggctttgtgg ttcaagtaat tatgcaaata tggcagatca
     9301 aaccaatatg aatatccgag tgatgataaa tcacgatatg atagcaaata caagtcccaa
     9361 tttctgggac ccgagggttt tgctaatgcc ctatgatggt ttcatttccc atacggatta
     9421 tgctgcaatg attacatccc aatacactgc tttgcaacct gtttatggtt atcttaattc
     9481 atccagcagt gacagtcatt ctttctggca gcacggtttt ccggttgttt atttctttga
     9541 atataacttt tcttctgtct atcacagcaa tgatgatact gttaccaatc ttgatcctgt
     9601 ttattgtgca tccgtaattc gtgcttcaac tgccgtaact gccagttttg ccaatatgcc
     9661 atctccgcct gcaaatctta cggctcagga tgaaggaacg ggaaatagta ttcacctttc
     9721 ctggaatcct gttcccgatc ctgatttttc tcattttcat atttcctggg gactttcgca
     9781 gaataacctt atttattccc aaactacaac caataatgaa atcagtatta ccggcttaaa
     9841 tacggatcaa ctttatttct ttactgtatc ttcagtagat ttttccggtt ttgaaagcta
     9901 ctctttggca acacagtgta ttcctaggtt aatacctcaa acacctgtta gctttataga
     9961 tcagccctta ccagaaatag ttaagctttc ctggcagtca aataccgagc ttgatttagc
    10021 cggttataaa atatatcgtt ctctaaatag ctcagaaacg ggagcgctta ttgctacttt
    10081 acctccaact ttatgcagtt attcagattc taatgttcag ggaggtgaat tttactatta
    10141 ctatcgtctt tgtgcctttg ataatgataa taatagcagt ccctttactc cagtgcttaa
    10201 aaccagacct ctcagtatga actgcggact tttaattatt gatgaaacgg cagattttag
    10261 cggaagcagt ccttttcagc caacggatga ggtagtagat aacttttacg atttgttaat
    10321 tgacggcttg tggaatactg cacattttga tttggctgat tttacagaga cattgcgttt
    10381 atgcgatatc ggtatttatt ccgtcattat ttggcacggt aacgattttt ctaatatgat
    10441 ttatcccaat caagttaaag atgttttaaa gaactatatt tattatggag gcaaagttct
    10501 tttcagtgtt ttccatccgg gattggcttt tgaactgaat gctgattatc ctaccgtttt
    10561 ttctgcagat tcatttatca atgaagtttt aggcatcagc ggaactgatt acactattca
    10621 agcccgattt aataaagcag aaagtgttta ttccggttat ccggatatca atgttgaccc
    10681 caataaagtt cccgcttctt ttaatggaca cctttatcaa gtagaagcat taaccgcaaa
    10741 tgcacaggca actcctattt atcattacgc ttccgcttac gacaataatt cacctcaggg
    10801 aattctcaac ggaggagtag taggtgttca aaacacttat ttttcaggta aaacagtaac
    10861 cttcagcttt cctctttact ttatggattt caaccaaagc cgtcaagttg ttcattacat
    10921 tatagagaat tattttggtt ttcctgttgc agttggggat gaagttaata tccctgttgc
    10981 ggaaatttct attttaccta atcaacccaa tcctttttct gtttctaccc aaataaatat
    11041 cctctgtaaa aacagcaacg ctcctttaca agttcaggtc tttaacctga aaggacagtt
    11101 aatcaaaacc ctttttcagg gaaaaccaga aggaaaaaat accctttcct gggatggtaa
    11161 cgataataat ggcaagaaag ttagctccgg aatctatttc cttaaagcac agcaaggtaa
    11221 taaggtgcaa acccgaaaga tacttctgct gaagtaaaaa taataaaaat aaatagttag
    11281 gggatgaaca ggattacaga gattttgggg attttgaaaa gagaaaagaa gaaataagta
    11341 agggataaac aggattaaaa ggattttttc ttgttttggc ttacaatttt cacctgcctt
    11401 cccggaaacc acaacccact aactatttca cttacaaccg agtttttact gatttcccaa
    11461 tctaaaaaat tgtgagctaa aacggaaaca gggaaacaat atctcgctga ttgggacggt
    11521 ggtaatacag ttgagattgg tcaattgtta gtttgtgctt gacagataat gtgaatgata
    11581 tttaaaatct tttgcagtaa ttgaagattg tttgttcaaa tcaatactgg agtaaaaatg
    11641 agtaaaaagc ttgagaacaa ctcaacaagc attggtagaa ttattaatct actttaccgt
    11701 tacttaatgg ttgcgtcttt cattacaata atagcagtgg actcgcactc cgagttgaat
    11761 gccaataata tagttcatga acccaactct cagctgaatg tctttcagga agatttcaat
    11821 tacttttata gaatattact agattctcat cctggcatag aaactgataa tactagtttc
    11881 aatcagaaag ttgaagcatt catggaatgt ctcgccactg taactgatac tctgcaattt
    11941 gaagccagat tgagagcttt tacccatgat ttacatgacg gacatacaag agtatcatca
    12001 ttttccaatc agggatcacc aatttaccct ataggattgt ggtggcatga atctggatgg
    12061 tatgttgaaa tgatttctac cgagttcaaa gatttaattg gaagtaaagt aataagtatt
    12121 aatgggatag atgctgagac ctttatgaca aaattaatgc aactaactac gggggagaat
    12181 gtctactggt tacgagacca agtgtctttt ttcctcagaa actcagcgaa cttacatgcc
    12241 ttgaatctgg atagacccga cggtgtttta cagttgatca ttgaacagaa tggaaccttt
    12301 tccgaagtca atttatacga agttgaaaga ccttctcttt ggggcaacca gaaaaccggt
    12361 attacggctt tacatgatga taactattgg ggacaagccc ttccagaaga caatgtatac
    12421 tacatccaat tcaatgcaat gtcagatcca gtagggacat ccgatactcc attttcaggg
    12481 tggatatcat tccttaaaga gagctttgct ttaatagaca gcttagggat agataatctg
    12541 gttattgatt taagaaacaa tagcggtggc aattctacta tgggtgatat actcctttct
    12601 ttttgctcac tacctgattc tattaaatat ttcggtggga agatgaagat aagtgatttg
    12661 gtgctcaatc attatcaaat ggatctgact tcaatgaata aggcattatc ctctgacttg
    12721 ggttatgaag tattggaaac tgaattgccc tttaccgtga ttttttatga tggtaaatct
    12781 ccatacctta gacctcggac tcttggtgaa atagttccca atgttatgag taatgattca
    12841 ataaaaaagt ataatggaaa attgatcctg cttactggaa gtcaaacata cagctcggca
    12901 attatgtttg ctactctttg tcaggataat ggactggcta tagtgtacgg aagtcctacc
    12961 ggaggaaaac catcttctta tggagaggga ctaaacttca gattgcctaa tacgggtata
    13021 atgtgcagtg tatctgttaa atactttcaa cggcctgata caagcagaga tccttctgac
    13081 agtctatacc cagatgttga tgtactgatc accccggaag aatatttttc acccattgat
    13141 acactctggg aaagggttct aaatgacata cgcaactcca atctatataa ataatctttg
    13201 tcacagaacc aaattcagag agaatgattg taagttatat tatattttgg ggaaatacct
    13261 aagaagtgca ggaaagtact gcttgatgtt aacatcccct ttcactcttg ccatttcgta
    13321 ttgatcaagg gctttaatga actgcctcac atacacaaat ccctgttctt catcccattt
    13381 tagaattacc tccgcttcat agctaccata atacatcaag accagtttat tggtaacagc
    13441 tcggatgaag cactcatggt tgttttctac aggatatttc tctaactcaa tattcatact
    13501 tctgatatac ttttctaatc cttaccattc taacatattc aggaaaatac tttggttggg
    13561 ataggtctat ctatatggaa ctgaagaaaa ctgccgatta tttggtaatg ggagccattg
    13621 cctttaattt taaaacgctg cgtaaaaagt atgcaaacat aatgtaggtg agcgtgccca
    13681 aattacggga aaagggtaac aaataaacga aagagagggt tttgttaagg gaaaaagtaa
    13741 aaatcaggta aatattctgc aatcttttga atatttctcc catttaatca taaattatca
    13801 agataatgtc gttgtcccta atcgttcaat ggtcttgtaa tattatgtct tatctcaaaa
    13861 agaattatct tttatctttt ggcgggcagg gatcgttacc gaatgatctt ttatcctgaa
    13921 tttttccatc tgacgaatga atcactaact ctgctttttg gttgttagcc aattttctgg
    13981 catattgtaa agcagaatat tttgtggaaa acactttaac ggctttttgt gcattagctt
    14041 tttttacttt ccatccctgc ttatttttag ttaaatgata ttctttataa ggcattttga
    14101 cctccactta aataattctt aatattaata taagcattga tatctatatt ttttgtcggt
    14161 aaaccataaa accctttggc aatcatttct ttgtaacaat cccaaacttg attataagtt
    14221 agcaaagccg gttttctttg atagagcaat tgcaaaagat ttattgtcca taggcattta
    14281 atattgaact ttgcgagaac tttatgagta gctttatcat ctgtgcataa aatcctgtca
    14341 gttttgtttt ccaataaata gacaactgaa aataattcgc ctccatccag atttataagc
    14401 tgaggaactc tattaatggt caatttacta tcatcaataa ccttaattgt ggtatatagg
    14461 tgtttgttaa aaagagtaag attacagcga tccgggtata ttttactgcc ttttatgtat
    14521 tcattatgaa cagtttccac aatttccatt tccaaaggat attcggaaag tttctgaatt
    14581 aagtccactg aagagcatct atcaatacag tttaagagaa cagtgttatc taatattaaa
    14641 atcattttaa ctatagccct tacccagaat tttgcctatt ttacggaatt catcattgac
    14701 ggactgaata tcagataata aatatttagc agctttggag atagaaattt ccccttttgc
    14761 caaggcaaaa tagaccagta aatgataaat agcggggaaa tcgttaatag ctattttata
    14821 ttcatattct ttaatcggtt ctttcaatct ggctatttca tctttgccat aattcaatat
    14881 atttgccttc accagattat aggcaatgca ttcgtaagaa acattaaagt attttgccaa
    14941 ttccctgatt tgttcagctg ttatcatttc cgaagaggat ctttcattta cattgtcaat
    15001 tagcaatgca taagaaacag taaagcgtga agcaaaatca tttgcctctt tttctttaag
    15061 gtctctggaa ctaatacgag aaataataaa ttcatcagtt tccaaatgca tcaatatatg
    15121 ccccagttca tgagctaaag aaaaaaagtt tctttctaat gtgtgccctt tattaataaa
    15181 aacacaataa acaccatctc gttttatagt aattgccgat agatttttat ttttgaatgg
    15241 tagggcgaag acaacaactc tccattttaa ccacagagct tcaaccaagc tattataatc
    15301 agttataaca ggcagttcaa gattagcttt gattatacct atttgctgat cagatatagt
    15361 tttactccta aattcatctg gtccgtgata aaccaaatta aaattattaa tatcagctac
    15421 atatatcaaa ttatcaacta tttcctggaa taatgccact tcttcctttt cttcttttgt
    15481 tattctgcct ttacaacgat aagcagagta agcaatatat tctttatcag taaccaagta
    15541 ttccagagga accttaataa tttccgatat ttgcattagt gttttcaagg gaacttgtct
    15601 tttaccctgt tcataatagg atatcaaagc tggtgtaaca cccaattttt gggcaagttc
    15661 tttaccggaa atgtttagtc gctttctata ttttttaatc ttttctccga cattcattgt
    15721 tctctccttt cttattaact atatgttaat ataatcaagt ttaggatttt gtcaagtgaa
    15781 taattattaa cattacccat ctctaaaata accctaattt ttttaatctt ttcagatata
    15841 tgtaacaatg gataacagta tcattatagg ttcttgtttt aactcacaat tttttgattg
    15901 ggaaaccatt tgtagatcgg ttgtaagtga aaagcaagag gggtgccgat aattgggaaa
    15961 gcgagtaaaa attgtaagtc aaaactccat gcgagaaaaa attgtaagcc aaaactcccc
    16021 gcgagtaaaa attgtatgtc aaaaccccca atgattgcga tggaggtgta aacccatagt
    16081 tcaaggaaag tataagttcc tttagaaacc ttaaatttcg ttggcaaatc ggaatttgct
    16141 aacccatttt tccaaatgca ttatttgggt taaaacagaa taatgctgat taacaaacct
    16201 ttttaccaga gttaaactgc aataatttgt tccagtttat tactcctttg tcgcaaaagt
    16261 cctggataaa cttcgcagta aagttattga ttaatttaga gtattctgca tagaaattat
    16321 catttttctc tttagcttta tagcccaagg gaacaatgat ttttgtatac atctcttcat
    16381 cacaggaaat gaattcccaa aaacgctgac cacataattt gaaataagaa cccttatccg
    16441 gtttatcatc ttttccgtag cagcaaccat ttattgcaat cactgttgtt tctttattat
    16501 tggaacgaat aattctggaa gttctattaa aatcgtctat catttttgcc acttgacgac
    16561 tattacccca attaggacct gactttatag agacaatata ccgcttgttt tctttgttaa
    16621 actccaaatc tatacccgga gaagatgatt tcactccacc ataaatcatt ccacaaatat
    16681 agatagctaa cgattccaga aattcaccaa acattgtttc ttctgcagaa gataagtgag
    16741 catccagaat ggtttttatc aattcgccgg cattaagaat attctttgct ctaaacagat
    16801 acggattttt atgttttaat atatttgcca gtttcaaatt gctgatattc ataatcctgt
    16861 tgttgtgaaa aatctcaata tgattttcta catattcatt caagtcattt tcatcaatca
    16921 cgaataacct cgcttaaaac aatacttgtt gctgattttg aattttccct aaaactaagt
    16981 tataatattc aggatttata tctattccta ttgcttttct ccccatattc aaagcagcaa
    17041 aaacagcagt tcccgaaccc ataaaagggt ctaagaccac atcacccggt ctggtaaata
    17101 gtttaataaa ccattccggc aaagcataag gaaaagttgc gctatgattt ttattaccgc
    17161 attcagtagc caagtgtaaa acatttgtag gatagaccat atctttacca acccagttgg
    17221 atacattttt accaaaacca ctgcctactt tggaagtatc tctaatttta tccgtctggc
    17281 ttaagttaga aagtcgtttt tctgcccagt cacctatagg aatcattact tcttcctgat
    17341 acatcttaaa gaatttgttt ttattgaatt gtaataatct ttcccatgca tcccgaaatc
    17401 tgttaggcca ttttcccgga tagcaattct ttttatgcca aataaattct tctgtccaaa
    17461 gccatccttg ttttctcata ttcaaaatca attctatcac ataggtatgt ctttctccgt
    17521 taactacttt ttctttgata ttcaaaataa atgttccagt gggttttaga attcttaaaa
    17581 gttcttgtgt tatgggaaga aaccattcat tatattcatc cggttttatg cccccgtagg
    17641 tattagttct actatctgca taaggaggag atgttactat taaatcaatt gactcttttt
    17701 ctattgtttg cagaatttcc agagcatcac caaggtataa ttcatattca ttattgttca
    17761 taaagtcact ttacctcctc acttataata taattccttt tacacatttt cccaaaacaa
    17821 gtcaagaaga aaaaataaac cgtatgtcag gagtcgtcac caccgatata acttttacac
    17881 aacttgagaa tttatttggt ggtgaagaat tgtaaccgct cagaacaagg aaattaggac
    17941 aaaacttata gttttaggag aagctttaag aaaggactat aattataaat ccgtgttagc
    18001 cgtataatct gtgatagtta gcactactgg tcatcaggag tcatatacgc tcggaaagat
    18061 atttttctta aacgaagtta gtttggtgta aatgactcct gacttaataa gtgtttcggt
    18121 tttttaacct aaagtagcaa ccatcacagc tttgatagta tgcatcctgt tttcggcttc
    18181 atcaaaaaca acggaatggg gtccttcaaa gacctcatcg gtaacttcca tagcagtcaa
    18241 accgaatttt tcgtatatat ctttgcctat tttggtcttc aaatcgtgaa aagaaggcaa
    18301 gcagtgcata aagatagtag tgctttttcc tgttttttta agcatttcac cgtttacctg
    18361 atagggtttt agcaggttaa ttctttgttc ccaaacgcta tccggttcac ccatagagac
    18421 ccaaacatcg gtatatagga catctgcatc tttaaccccg gcatcaatat tgtctgtaag
    18481 ggtaattttg gcaccggttt cttgggcaac ttttaggcat ttgtgcatca gattttgttc
    18541 gggaaaaagc gttttcggac taacaatccg gaaatccatt ccggttttac aagcacctat
    18601 cattaaagca tttgccatat tattacgtcc atctccgcaa taaacaaaca ccatttcgtt
    18661 taagggttta ttcaaatgtt ccagagcagt taaaaaatca gctaatactt gagtgggatg
    18721 atcctgatcc gttaaaccgt tccagaccgg aacaccagca tatttagcca gttcttccac
    18781 aatttcctgt ccaaatccgc ggtattcaat gccatcatac attcttccta aaacccttgc
    18841 tgtatcggca acggattctt ttttgcccaa ttgtgaaccg gtaggaccca aataagttgt
    18901 atgtgcacct tggtctaaag cggcaacttc aaaggcacaa cgggtgcgag tgctgtcttt
    18961 ttcaaaaatt aaagcaatgt tttttccttt taatttctgc tgttccgttc cagcatattt
    19021 ggctttttta agctggatag caagatcaag caggaattga atttctctgg gagtaaaatc
    19081 catcagggtt agaaagttac ggtttttaag gttgaaagac attttggtct ccttttagtt
    19141 atttatgcat ttaatttatt tttttctcgg atttccctaa caggaggtag atttataaaa
    19201 ccaataacaa acatattcag attgtatccc ctttttaaaa atacaacccg actgtcaata
    19261 agtttctctt tttggctgct gaaaaactat atcttggcac ttttttaccc ctattcaaaa
    19321 taagaatata aataacataa cttttgggca gacactatat taggagatat accagacaag
    19381 agattccctt atgcttccca tattgcgata agggaatgat atgggaatca tatcgcgtgc
    19441 acaacttcta taaccaatat ttgattagtt gcggttacta tgatttttga cagccaatat
    19501 tatgcctaag atttgtcacc aaaacttcaa tttgtcaccc ctaatataag tgagagacaa
    19561 aaaaaggagt aacaaaaatg aaagttaatt ttcaatacgg actggctggc tacaccggta
    19621 aagctgatgg actggtattt tgttttgacc ggaaatccgg tattgtttat gccaggaaaa
    19681 gagtttatcc ccgtttaacg gaagaaaacg ccagaattgg caatatttct cacaacatat
    19741 tttccttaaa accctcggaa gaatacaagg atgatttgcg actttattta atgggttaca
    19801 atggattacg ggcaaatagt cataaacctt tgcgctcttg ggtaaatttg tatcatcgct
    19861 taatggcaaa tatggctaaa aagaatccgg aaatagacct gaaaaccctc acccgccaac
    19921 aaatctttgc tgataatcta ccctgcaaaa gcattaaatc cgccgttgaa gcaggcattc
    19981 ttccacctat aaatggctat gaaagaatga ctgctttaat ctaacggaaa aatgatgtcc
    20041 tcgtcgttta ataaaaatct acttatctct ctccgcttaa catttccatt tcaatttatt
    20101 ttattatttg aggttaaagg aaatatcaat cttacggtgc aaaaaatgat tgacgccaaa
    20161 ggcaactaag aaacagaagt aattaaaagg tctgtgccca gtgaagaaaa aaagttgcag
    20221 gatataccgc ttaaaaaggt ataaataaag gaaccgttta aaaagacaca gacgaggaaa
    20281 aggaaagaac aaagcgaaat agaggaaaaa tgaaagttat cacagttata ttaatgctat
    20341 tgccattgct tctggcagct caaagttata ctttggaaga acttataact tatggcttgg
    20401 agaatagttt cagtgtgcaa aagagcgctc taagttatca gagcgcaaaa tcaaccctga
    20461 atacatccaa atggaattta ttaccagatg cttctgtaag tggcggaata aaaaaggact
    20521 ttgacctcgc tgtcaacact gatgatgtaa ccagttcggc tggtttatct ataggcaaaa
    20581 ccattagttt aaacgatgct gcctacttca attacctgta tgcctctttg gataccaaaa
    20641 ctgcaaatat gaaactggaa cagacccgca gagcttatgc ctatgaggtc ttcagtgcct
    20701 atttaaaagt gctcagttca caaaagcaaa gaagttctct ggaagaaaat ttacagattc
    20761 aaattagggt ttgggaacag agtaaaattt ttttgcaatt gggcaaaacc actccctttg
    20821 aggtaaagca gaatgaaatt gcggtcttaa attccaatat ttctttaatg aaactggata
    20881 atacaattgc caatgcccgc aaggaacttt ttgctctcgt ccaaatgcag gatgaagatt
    20941 atcctttaac tgagctggaa ctgaatatcc aaaaagaaat tcctgcgttc aatacggaaa
    21001 aaaatgcgga tctcaatatc ctcaatcagg aactgaaacg cctgaatctg caattaaaac
    21061 aaaacaaact ggatgatttt ccccgcctca atctggctta taattttgac cgttccgtaa
    21121 gcggtgccga ttttgatttt gaccattata acaccaatca cggcttatct ttaactttaa
    21181 gctatcccct cttcaatatt ggggaaaatg ccgaaagcag aaccagaact aaaatcagca
    21241 ggcaactttc ccaagtggca attgaagaaa agaaagatca attgcagcga gattacgaca
    21301 gtgctttaag agaattgcaa tatttaaaaa gaatgaatga actttacagc gaacaactgg
    21361 ctcaatcccg ggaacaaatt acccaggcag aagaacgtta tcgcttaggt cttattgaac
    21421 ttctggaact ggataaaacc cgcaccaact atattgaaac agacatctct tataatacaa
    21481 acctatattc tattatcgcc aaacaggaag aaataaatta tcttctttcc gaacccgttt
    21541 taggaaaatg gtaaggtaac ttctatatat ttaattgata aggaacacca atgaaaaaat
    21601 accttaagta tatcatcata attgcgctta ttcttatcgc cataatcgtg ataatatcct
    21661 tgcgcaaagc gaaaaacaaa cccgaattca gaacaacatc tccctccttt ggcaatatca
    21721 gagaagttgt taccgctacc ggttccctaa atccttatgt tttagttagc gtgggcaccg
    21781 aagttagcgg aaaaatagat aaactctata aggacttcaa tgataaagtg aaaaaggggg
    21841 aattgctggc aaaactggat acggaaattc ttgctaccaa tctggaagcc gcaaaagctg
    21901 atctggcaaa ggcaaaaacc gctttagacc aggcaaaact ggattacgat ctgcaggcag
    21961 aattgctgga aaaaaagatg actcctgaat atgatgccaa aaaagcactc tttgcttatc
    22021 aaacagctca acaaaacttt aacaatgctc agctcacatt acagcgcgct caaaaaaatc
    22081 ttgccaatgc ctatatcacc agtcctataa atggaattgt ggtttcccgc gatgttgatg
    22141 aaggacaaac agttgcagcc agtatgagtt ctcccacttt attcaaaata gccaataacc
    22201 ttgatcagat gcaaatatct gctaatgtgg acgaggcaga tatcggaaaa ataaaggttg
    22261 gcttgcctgt tgaatttagt gttgatgctt atcccagcga aaacttttcc ggtaatgtga
    22321 aacaaattcg gcttaatcct acaacggaat ccaatgtggt ctcctatagt gtgattattg
    22381 atgccgccaa tccagaacaa aaactccttc ctggtatgac taccaatgta actatcatta
    22441 ttcaagcccg ggaaaatgtg atgcgtattt cggaaacagc tacccgtttc aagcccagta
    22501 aagaaatttg ggaacaactt ggttataaat ggagcgacga ccttttacta aaagcccaaa
    22561 aagaagctta tgcttctctt gctaaagcaa aaactatacc cgcaaaaact gatagcacta
    22621 atttcaacca aaagaaatac ggtaaaacaa aacaagaatt tagttctaaa acaattagtt
    22681 cttcaggtaa agcggcactt atttgggtgc tggaagataa tcaacccaaa cccaaaggaa
    22741 ttagaaccgg aatttccgaa agcggatata ttgaagttat agaaggactt acaggtaacg
    22801 aaacaattat caccggagta aatactaaaa gtggctctga aactaataac ggaactcaag
    22861 gtggtcctgg aatgaggcac ttctaaaatg ccaaatctgc taattagaac agagaattta
    22921 accaaaattt atcagatggg agaagttcag gtgcacgccc tcagaggtgt ttccctccaa
    22981 attaaagaag gtgaatttgt cgctattatg ggtgccagtg gcagcggaaa aacaacctta
    23041 atgaatcttc tcggttgtct tgacctgcca acaagcggtg aatattacat caatgatatc
    23101 ggtgttcacg aaataactcc cgccgaactt accaaaattc gtaatcagat gattggttat
    23161 gtatttcaaa acttttattt gctcccgcgc acaaccgctt tggaaaatgt ggaactccct
    23221 ctgctatata gaaataaaat gactcatcac cagcgattgc aaaaagcaat ggaagccctt
    23281 tcaaccgtga atatagagga tagagcaaaa cattatccca atcagctttc cggtggacaa
    23341 caacaaagag ttgccattgc ccgcgctatt gtaaatgatc ccgtttttct acttgccgat
    23401 gaaccgaccg gaaatctgga taccagaacc agtttggaag tgatgactat ctttcaggaa
    23461 ttgcataaaa acggaaaaac cattttgctg gttacccatg aacacgatat tgcaatgtat
    23521 gctcaccgtc tgataacttt tcgtgacggg cgcataattg ccgatgtgcc caatagcaat
    23581 cctcacgatg ctgcttatga tttggcaaat atgcctaaac ttcccggaga tgaagaatga
    23641 gtatagtagg aattattcgc atcgctttca aatcgcttgc ccgcaataaa accagaactt
    23701 ttttaacaat gctgggaata attatcggcg tggcagcggt tataacaatg ctggctatcg
    23761 gacaaggtgc caaaaagatt gtagaagatc aaatctcttc tatgggaacc aatgtgctga
    23821 tggttactgt aaattttacg cgtagttcta caaatgtgcg tcaagcagcc ggaagtggaa
    23881 gtttacttga acttgatgat gtttttgcta ttcgctctca ggttgaagat gctttgtatg
    23941 tctcacccgt ttataacagc tttgctcagc taaaatttga atcgcaaaac tggagaaccg
    24001 gcgttatggg tgtagatacc gattattttt atatccggaa tatgaatcct gaacaagggg
    24061 aattgtttta tgacagcgat gtggaaaacg gaactaaggt ttgtgtaata ggcaaaacag
    24121 ttgccgaaaa cctgtttgga gatatagatc ctgtgggaca aataatccgc attcggaata
    24181 ttcctttcac cgttaaaggc gttatggaat ccaagggaca aaatgtgatg ggtgcagatc
    24241 aggatgatag tgtttgggca ccttacacaa ctgtctatac ccgtttgctg ggtagaaggt
    24301 ggagaaatat gatgatcatt gtttctgcgg tctccaaaga taaaattgct gaagtgcaac
    24361 aggatattct tgatctgtta caaagccggc atagaggaac taccagcgaa gaatttttag
    24421 tccgctctca aacagatatt gccgaaacag ccagtaaggt ctccgataca atgacttttc
    24481 ttttggcaag cattgcgggt atttctcttt tggttggggg aataggtatt atgaatatta
    24541 tgctggtttc ggttacggaa cgcatcaagg aaattgggat cagaatggct gtaggcgcag
    24601 gaaaaagaga tgtcctgtta caatttatta tagaagcggt tacaatcagc attttaggtg
    24661 gttttatagg tatccttctg ggttttggag cttccaaaat tgtgggtaat gcaatgaaat
    24721 ggagtgtggt agttacacct ttttccgttc tgctttcagt tggattctca atggctatag
    24781 gtatcttttt tggctggtat cctgcttcca aagcggcaaa tctgaattta attgatgcct
    24841 tgcgttatga ataaacaatt aaaaactaag ccccaatttc ccaaggtaac attgtaaacc
    24901 tgaatttggc aattctataa acaaagaaaa ctcgcctgca agtgatattt tgcttgacga
    24961 atctcaactc cctaaaacta tgaagaaaaa aagaaaggag acacaaaatg gctgtcaaaa
    25021 ttgataaaga aacctgtatc ggatgcggtg cctgcattga tgtatgccct gtttctgctt
    25081 taaaattggt aaatgacaag gcagaatgtg acgagtcact gtgcattgat tgcggggctt
    25141 gcattgatac ctgtcctgtt caggcaattt cacaataact gaattcaaaa taattatcgt
    25201 ttcccaaagt ggcaaatcgg gatgccaaat tccgatttgc cacgcggaat gcaatttcta
    25261 atattttatt atattttcaa gtatgagtat aatttagcga agagtaaagc aataacatca
    25321 aactttagag gaggtgatta aaatgaagcg cttatttctt attcttttta ttacaactct
    25381 catttccctg aattttgcca ttcaggtaag cggaaatcaa agcggagttt ggtctcctgt
    25441 aaacaatcct tacgaagtaa ttggagcaat aactgttcct gcatccgatt ctttaattat
    25501 tcaggcaggg gtagaaattc accttttggg ctcctatcaa attaccgtgg agggaatatt
    25561 aaatgcctat ggaacagagg tagatagtat ccgaatttta aatatgcagg caaatccgac
    25621 tgcactttgg cctgggttgc gttttgaaaa tctgcagcat accagtcact taaattatgt
    25681 ttatctggaa tatgccactt atggagtgcg cactatcaat tcccctatcg ctatctctca
    25741 ctgtcatata aacttatgtg aaaaaggaat ggagttttat ggcatcgggt cggcagttcc
    25801 tttgcctaat atagtagagt attgtttaat tgaaaactgc acccaaaatg cgattcttgt
    25861 cacttccaac agcaatgcct atatcagata taatgaaatc cgttataatg gaacgggagc
    25921 tcaatttcgg gcagctattc aaatgggcaa tcaatcaggc gtaaatcagt gcaatcccaa
    25981 tatcttatat aatcatattc atcacaacta caagcaggga atttcggctt gggatattgc
    26041 ttcttcgggt tctatcaatc cgcaaatttt gcataatatc attgagtata actacaccgg
    26101 aatctatctt ttgcaggcat caggttatgt agctgataat caaattaact acaattttat
    26161 tcctggagat atgaattccg gagcaggagt aatggtttcg ggagtaactt ccgtgcccta
    26221 ttttgaacgc aatcatattg agggtaatta taccggtttt tacattacta ataatggcaa
    26281 acctgttttg ggagatctaa ctttaaatca tatttgggct cagggcgaaa ataccattat
    26341 caacaatatt gatgttaaca atcacaataa ttccgtttat tgcgatgctt atcctaatgc
    26401 caattttatt attatggcag aaaataacaa ttgggggttt gctacggcag cagaaattgc
    26461 cttaacgata aatgaccaca atgataattc cgctttgccg acagtggatt ttgaaccctt
    26521 tattattccc ctgcaaaata caaccatcac aggcagttat atttatactg gaacagcaca
    26581 aattgccaat gcgcgtttgg aattaattgc agtaaccaac ggtattgttc aggtctccat
    26641 tcctcttact acagctgatt tttccgttca ggcaaatatt tccgaggatt tttatgcgca
    26701 ggttgtttta accgaaacgg gaacaggaaa agaactttat ggctgtgcag ggagttatat
    26761 taatcctggt gttttttctc ctgctcctgg aaattcagtt gatatcggca tagttgaagt
    26821 taccgatatt cctctttccc gttatgaata tgtaggagaa ccgattcagg agggtaattt
    26881 aacgctatat cctttgctga caggttgggg tttatacagc tatgataaat tggactgggt
    26941 ttatcctgca ggtgattatc tttacctaaa aagaaatatc tggcgaactc ctgcgggaga
    27001 agtgacaaca gaactgcctt tgggcactgt atataaaaag tatctcaata tcaatgcggg
    27061 agatagctgg cagcaaactg aggttatcta tcctggcaat atcactttta ctactgatgt
    27121 tcaggttact aattgtgtta cagatatttc tacccctgca tttttgcttt ttacccgtaa
    27181 ggattcattt ggatatgttg tggataaaag aataaaagga attgctgaag aacttttcca
    27241 ttaccaaaat cattacaaca ttgccaggga aaacattatt tacctcccag gcaataatga
    27301 ccctttaact acaggaaatg ttactttatt tatgcctgaa ccgctaagtg aaagcccttc
    27361 ttttcttgcc tttgagcccg atattgatga tcaaattcat cttttttggc aggcacccgc
    27421 acagggaaat ttcagctgga cttcttaccg catttataaa aacgatgctc taataggcga
    27481 aattcctttc tctcaaagcg attatatcgt tactgatttt aatcctcaaa ttactactta
    27541 cttttatgta actgctacgg acggaataaa tgaatccgaa ccaagcaact gtgttacagt
    27601 atattatgtg ggtaatgaag acctcattca gaaacctttt agcgtagatg tatatcccaa
    27661 tccggttagt tttacctctg gctcgggtct tatagtggaa gttaaaaatg cggaaaacag
    27721 ttctgccctc atagaaattt acaacctgaa agggcaattg gtatttagaa aaaacctgca
    27781 ggacgaaaag aactttctct ggaagggaat agataataga ggcaaacgca gttctgccgg
    27841 catttatttc ctgaaaataa aggttcaagg cgaaaaaccc gttacccgta aaataatggt
    27901 cttataaata aattccaaat tctttgtaat agaaataaaa agggtggcag aaaatgaaac
    27961 tgtcaccctt tattcttgtt ttggcttaca attttcacct gccttcctgg aaccacaacc
    28021 ctcaatttgt aaacacttac aaccgcgttt tcactaattt ccaaattgaa aaattgtgag
    28081 ctaaaacaaa agctattccg gaaacatata tacggagagc aaggacttat caaaaacata
    28141 atactgttta gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccggaaac
    28261 atatatacgg agagcaagga cttatcaaaa acataatact gtttagcggt tttggttata
    28321 gctttcttat ccagtttgtc atacagcaca ggataatcca gaaaacagtc aatcggtaag
    28381 ccgtaagaaa tattctcact catattattc acccagtatc tattggactt gatgttttct
    28441 tcatactttt tatgcagagt ggttttagtg gattctatat atttatccgc aaaaagtccg
    28501 tttttcaagc tatccaaagt ggcaaagata gcggcgttca attccttggc tctttcagga
    28561 tcacaaccca tcaaagttaa agtgaaaaca ccaggtttag gcagaaagtc ataaaaactc
    28621 tgaatttcaa caacataagc accgctcata ttttcgcgga cattttcccg cagtttttcg
    28681 taagcaatca tctgcagagc ggattgttgc acattgtttt gcggggaaaa cgatgcctta
    28741 ttgtttgtta cattagatac aaaactgcgt tcagtgcctt tatggaaact gatttccttt
    28801 ttcccgttaa aagctctaac gccggcatcg gtcatttttt cctttcttcc ctttgcaggt
    28861 aaattggcta aatatgtttg gcaataatcc tttaattggt cttcagcaaa attgccgaca
    28921 acataaaaag tgaaatcggt ataatcgcca aagcggtctt ggaaaatatc ttttaactgt
    28981 ttcagagtaa cgctatccag gtcttccgga tgcaaatttc ttttcagagg atgattattg
    29041 aacatcagca catttaaagt atcaaagaag gcgttggatg gttccagcaa atagttttga
    29101 acccgagccc tggttgaggc aacagctgca gaaaatgctt cttcattata tctgggagca
    29161 taattatatt gatagagcat ttggaacatc aattccaaat cttgcggaga acaagaacct
    29221 ctccaaccct ctgaataaga gtaaattgtt ggataaacat acacaatttt gcctgctaat
    29281 gccttttgca aagcaggagc atcaaaatta ccaaaacccg atacactgaa atattgggat
    29341 aataagtcgg cagatttata attttccggt ttcagttttg cctttccccc gggactttgt
    29401 gctattagaa taacctcatc tgccttaaaa tccgtttttt tgctgtaaac agttattcca
    29461 ttggaaagaa cccattgttt gattccggac ttgggaaaga ccttttcttt agttattttt
    29521 cccggaatgg gaattgtttc caaaagcggt tcattcacgg ttatatcttc ccagggttcc
    29581 agttcctgag caattgactg ctgataaata ttcagcaagt cctcgcgcga gggatatttt
    29641 gctcctggtt tatccgttcc agctaaactt aaagtaagat tttcagaggt aataacatca
    29701 tcaacaatgt cgttcacttc gcttaaggca atttcataga tcagcccttt cagcatttgt
    29761 tcataggttt cagcgctgag aattgtatct tcagacatta cggcatccaa aagctcccaa
    29821 attacatctt cggaatccat tgtcggttta tcggcaaccc atttttccgc ttgtctaatc
    29881 attatttgtt tggctcgttc ccattcacca ggttgaaaac cgtgttgacg cacccgcgtc
    29941 aattccgtta tcaaagttct gaaggcatct tcactgcgac cttcattggc aagcataaaa
    30001 caatccgtag cattaaaacc cttcagccaa gtagcattaa acataaaggc ataagaaaaa
    30061 gacggatcgg gttgttgact aagctcttcc aaacgggcat taatcatcgt gaaaaacaaa
    30121 tcctgtttca tctcattata caaagaaccc aaatcagtta caggagtggc cttaactttc
    30181 caggtgcttc ttatcattgt atagggctgt tctttatcca aaaccgttac cgccctcggt
    30241 tcaatattat cgggaacagg ataacttaag cggggacgcg gattttctct tttaggaata
    30301 acgccaaagt attccttaac taaaccttcc aacttttggg gatcataatc acccacaata
    30361 aagacggtct ctaaatcggg acgataccaa tcttgataat aacgaattaa actctcgtgc
    30421 ttaaagttct taaggttttc aattgttccg ataggattcc tttcggcata acgagaaccg
    30481 gcaaaacgca ctttatctat ttggtcttga attcttcttt gtgcattttg tcccagacgc
    30541 cattcttctt gcacaacacc tctttcgcgt tcaatttcag cgggctcaaa acttacttgc
    30601 caggcaatat ccgaaagaat agaaattcct ttccgcattt tggcttcatc atcagtagga
    30661 agcttaaatt cataaacagt aaaatcataa ctcgttccgc catttaagcc attgtgataa
    30721 cccattccaa tagaagtaag ataatccacc atttccgttc tggggaaatt tttagtgccg
    30781 ttgaaagcca tatgttctac aaaatgtgcc aacccaagtt gatcatcatc ttccacaaca
    30841 ctgccggcat tgattaaaag ccgtagttca agtcgttttt ccggttttgc gttttgcttg
    30901 atgtaatatt tcaagccatt atccaaagtg ccggatatta catccgggtc ttgttccaaa
    30961 ggaaaacttg cgtattccgg aggaaggatt gcacctaaag tcagaataca gcatagtagg
    31021 attaagccga ggtaggtcct cttcataatt actccttgta tttttatttt gccctgatag
    31081 cttgcccgct ttctttagtt tggattaaaa ttaactacat attgttcgtc cactgccaaa
    31141 atgttttcac tgtcgcaaaa agtgtaatgc gactcatccg gaagatatat ttctacctga
    31201 tagtaatatt tatttccata atccacacgc aaaaatcggc tttctccagg caaaatatta
    31261 tacataacgc ctacctgata ataagttaaa ggactatgtt gccaaatttc tgcccagctg
    31321 attatttggt cagagttgtt tattactttc acactggcac gattaggatt cagataaata
    31381 ctgtatgttt ttcccgcttt cacttcaatc caagtagaat ccagataagc attttgatac
    31441 tcatcaaaaa tactaaaggt ctctcctacc agccaaatag gtagtttctt tttcacttca
    31501 ccagtgaaaa tattttgcgt gtcggtatct atattaaagc taacactttc ccctgcagga
    31561 attgtctttt gttccatatt ttccacttta atataagcag gatatgaaca acggttaaaa
    31621 acctgcaatt tccctccgga ttcacaagca gaaagcatta acagaatagc cgccaacccc
    31681 aggattaagc aaa
//
LOCUS       DAOOZV010000016        30426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1644_length_30426_cov_57.089263, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000016 DAOOZV010000000
VERSION     DAOOZV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 30426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30426
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_30426_cov_57.089263"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            592..1419
                     /locus_tag="PLW08_02995"
     CDS             592..1419
                     /locus_tag="PLW08_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51601.1"
                     /translation="MDFPKELIETLRLYNNQLSQDFQKKRIETEEAQKKEKLITNLLE
                     YLNKGWPMHFLTMADQQSDLLKRMKITNPYEIGILEYAYRFSKEETTNIFKRYPNLLE
                     EKCREFRITLDRNSSHPKYSVKNGFFKIEIVEARRVAVLSDYEGKLIELAADIEPVVE
                     ALRKEYIRVFDRKFQGIKFLKQLRSNYKIFIQKKHLPDGSSVPIKDIVKLMKKKNKKF
                     RLDEFIADLSSLVEKGPFEIEERTIDFQQTKDTKQGILLIDNSIRGYIGYVVFKEKL"
     gene            1416..2687
                     /locus_tag="PLW08_03000"
     CDS             1416..2687
                     /locus_tag="PLW08_03000"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPL51602.1"
                     /translation="MSDQNNLATNIVNVLRMGIPPQKGVEQYSVGNEKLIEGIKKFHL
                     TGMSNHGIIRFVSGSWGSGKTHFFRLLREIALQNNCLVSNVELDVNSAAMNKFQSIFS
                     AIIRMVATPSYYEEDKIDIVPFGMVIKESLAWLATGRHEAKYVNVPYEKYQKAVEILM
                     ADHSIDIDFKKMVDNYWKTFIIDSPDESAVEKTRGEILQWFCGEGSIGFYRKNFNINK
                     LITKENAKIMLQSLAGFVRLSGYRGLIILFDEAEQAFSIMRQSVLRDAHNNLLSLINN
                     IENVPGLFLIYATVPEFYSDPKYGIIRYGALSGRIGQPDPNRFPRALDKIWNFDAVQT
                     ELNDYQEVALKIKNIYLTAYPETENILPNDVEVKKRVEELHKKHSPYAGIKFWRLLVS
                     SMVEDFDNHAEGIVKSSDKLYYDVMERLKNE"
     gene            2689..3882
                     /locus_tag="PLW08_03005"
     CDS             2689..3882
                     /locus_tag="PLW08_03005"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPL51603.1"
                     /translation="MFVNNQQAQAILESLRKGIPPEGFVRTFTVGRKHEIDDLKKRLD
                     KDDGTVLLVKANYGSGKTHLLKFIKEEALEKNYAVSLVTIDSNSGVRFNRMDQIMGAV
                     CRNIQINSEKNEKGLAPLFNLFATNFKNDKLTNSYFNKISNYGKWDYSEILDSKAMFI
                     ALRAWTTGLQSAQDTIIDWFYNPSNYRESRKHLYQSLVLELRSYFHDPRPEWKFYDDN
                     VFVTNKNGYEQSWAVLRDINTLCKQSGLRGFIILFDEFEDVITNLNNVRYQEGAFWNL
                     FHFYEGKMFQGKTFFAVTPDFVNKCKRKLHIKERWDFDYARFDKLPMYEMSPLQLLEL
                     EELSLLIFDVHSIAYEWEPDTIMLDSYFKSVIKKAAEVPVQDRVRNVIIRVVEELDNL
                     YQELH"
     gene            3879..5990
                     /locus_tag="PLW08_03010"
     CDS             3879..5990
                     /locus_tag="PLW08_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL51604.1"
                     /translation="MNSINLLNSRVATAFYGRFNFINNAQEAVIEPLVNGRNIVLCSG
                     TGSGKTEAVMAPIVSRYWEEALEYDELFIIYICPTKALVNDLEKRLELPLSYLGLRIG
                     IRHGDRDDLKNNKIPHVLLTTPESFDVLLFKKETRILTVKAIILDEVHILYNNQRGLQ
                     LSILLNRLQILVQRKIQWAALSATIGNMSFIRDFIIGSNSQADFFSYPSERRIDCYIT
                     HIKNLYIFQQLIEKLIDNSKLLIFTDTRKECERLASFLNGSDFLSYRLFVHYSSLSRG
                     IRVETEKKFADSSSAVCIATSTLELGIDIGDIDAIIIWGIPNNIESFMQRIGRGNRRS
                     QKINVICLVPDTSETVFLDLIKFYILTECAINGNLSQENPYKLYGSVVQQSLSIIAAN
                     EGRFVKSEELMTAHSHLPYINKFIMDSILAELCDKEFILKHNFKYSYAAYEKLFQIKD
                     LRLIYGNFPLGTKMVRLMSGKKDLGEVPEINLVRIKQGSIVRFQGKYWCVKKVSTDLI
                     SLEPTSNKKNSIDFIYPSSKKRVDSYIINQVWFALHNDINYKEYYDSNLYTYIISRQK
                     LIRDNIPLLSIPYIRYNDKIKYYTFGSMIVNRAIALFMDCTEFEADDISLTLSSPLSW
                     NTIPENPMEYQIIFSKLFDNLEQQTIFQMQLPLELQLDEYLQYWLNNKTIEEILKRLK
                     ISIPLEINLDLVKKIDFYSLS"
     gene            6088..8328
                     /locus_tag="PLW08_03015"
     CDS             6088..8328
                     /locus_tag="PLW08_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51605.1"
                     /translation="MKRPFIILFVLLSAILFAGERGLTVKEIEVSGEVISISSPVQSL
                     IQVAPLRKVEKVKALKNWDTLQELNLNFRLPAKGQYGLQFIDLSQSVQPFGFPGCLSE
                     TAFQAVNKAPVWVQTELASILAELIPEKQTLWSNLILSAQDPYVDEICFCIANSSPQY
                     LNSAYALPELFTENAQLLYSIACELPYVEIMDTGTAISGGDYYSTTRYWKKNTSGEMQ
                     QITVPRELYYWYIVHPKLTDEIPAYINPAIIENNSTHNNNIAPPPTGKFWRGYLYNLQ
                     ESDYPVLRDTLMQCQTLFNRDGTGNDAIRTIQWWINQQMSFTSNNERPHQPVRIITKH
                     IGRCGEYADLTSAVARLSLIPCTSILSYSLDHTWNEFWDENWVAWEPVNGYIDIPLVY
                     ENSWGTVFGSVFEIHPDGLLTPVTDKYSEGVSTIIIQVTDANQHPVDGARVMLYVMQN
                     SIHFDCEQYTDNNGIATFLVGDNLEYFTRVETDFGSFPEIAGSYLTLTNNSVAGETYQ
                     YIITIPNVMPVPELEELPPPADPVQDYQFAVSYYSEGYYITGVTLWDDIYSLGVRPLH
                     YKYVATPGELSFMVMDSDNILIWQLDNFGSGYSYSGTASEGTASFSIPLGSDWYAFMD
                     NSLHHRNAVKINGGLLFQHTGTAVQDETIPNVLLQYSIYPNPFNKTTCLSLKADKDFK
                     ADISILNLKGQVVKSWQNTFIKQGNSDFYWDGKDNKGRDISSGVYFWKVQSGETAFTN
                     KILYLK"
     gene            complement(8973..9764)
                     /locus_tag="PLW08_03020"
     CDS             complement(8973..9764)
                     /locus_tag="PLW08_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPL51606.1"
                     /translation="MIYSDIYLGDNKFPLLAFAIHNGNYMPPELLQNCGIDADTRLRE
                     EDPYTNGFAECFPNRIIVQTSRFAVDLNRPPEKAVYQKPEDAWGLQVRKMPIADNLYA
                     KLLKAYADWYQIICYQIERLLKIHSSLIILDLHSYNHRRGGPDVAPDPQNQNPDLILG
                     RNNLPESVYPVVENLRVLLDGNPFQNIKLDCRCDIKFSGGHFSRCVNQTFGNKVLCLA
                     IEFKKIFMDEWTGELNLPAYFQLKEIFQTAVLKWMSEITSLEKKG"
     gene            complement(9761..10420)
                     /locus_tag="PLW08_03025"
     CDS             complement(9761..10420)
                     /locus_tag="PLW08_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51607.1"
                     /translation="MTRDFSEVMNTLSGIQDYLLQVAIYDDFKPECIIILEKDPPDLL
                     ESLDSLRKYSHQRQLRFPLLVNRKFVESSLDSYPLEFLDIITSNYQNLFVKEDILKNL
                     QFDPDDLRLQMEREIKGKWLLIRLSVLERKNTPKQLSDLLFIALSNIIPVLKGICYLY
                     DGVVPLKLEEILAKNNIITNVKFEPMLDWVSGDEATLEDIKQYLGILEGLMQYLEQLD
                     Q"
     gene            complement(10435..11280)
                     /locus_tag="PLW08_03030"
     CDS             complement(10435..11280)
                     /locus_tag="PLW08_03030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPL51608.1"
                     /translation="MNFQDLILTLQNYWAEKGCCLIQPYDLEMGAGTFHPSTFFGTLG
                     KKPCAIAYPQPCRRPKDGRYGENPNRFQHYYQFQVIIKPTPDDIQNLYLKSLSAIGID
                     ISMHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVASIDVFPVSVELTYG
                     LERIAMYIQNVDDYRLLAWNENIGYAELFYEREKEYSAYNFEEADTNLLFQLFQDYEK
                     ECNALLGKQLIYPAYDMLLKCSHTFNLLEARGVISVTERASYIARIRNLARACANAYL
                     TKYNA"
     gene            complement(11277..11672)
                     /locus_tag="PLW08_03035"
     CDS             complement(11277..11672)
                     /locus_tag="PLW08_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPL51609.1"
                     /translation="MKKYYYTIGEVSNLLGVKPYIIRYWETEFPGLNAIKSEGRIRKY
                     NEQQVLLLRRIYDLLYNQRYTIEGARKIIKQERTKIQTKTKETLDDSLSSPKKDSEMK
                     TGITEILQDIKKDLTLIQQTCKNYNQDKK"
     gene            complement(11669..13171)
                     /gene="lnt"
                     /locus_tag="PLW08_03040"
     CDS             complement(11669..13171)
                     /gene="lnt"
                     /locus_tag="PLW08_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPL51610.1"
                     /translation="MDLLWVLLSALMLALSRLPIHCGWMVFFGWIPLLYVLERRKVKL
                     WQAGLLFSVVYIGVVMYWIAGVTFFGLIGIAIVYFLFYWLCFYAISRIGRFYPRLFYP
                     NFIAVMLSFEYLQNFGETRFPWFNNAYSLSDYTVLIQAGDLGGVILLSLLILLINVLL
                     YEVLLKKKTQCLLSVAGIFILWIGYGIYCLNYLPVQKQDPKITVMQPSIPQDEKWDES
                     AYQQILHRYDALCAQAKKDSTKLIIFPEAAMPVYLMYDYRARNFLKDLMQKYELEIFT
                     GFPHFTLAPENHINSELYYNAAALFHPDGSISELYYKNILVPVGERMLWLNIFPFLWK
                     LQFGQANWEFGNGPVYYQTDSLTFSPSICYELAFAEFHQQMAIRKDKTTGKLKKADFL
                     VNITNDAWFGTSYGPWLHSIMAKFRAVENRIQIYRSANTGISLIVDPFGRIIAKAGLF
                     EITNLQAPLYRCSRIPVYRYIYWYPWIFVILAGGLFLFSFTKKKQKGEVL"
     gene            complement(13175..14011)
                     /locus_tag="PLW08_03045"
     CDS             complement(13175..14011)
                     /locus_tag="PLW08_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51611.1"
                     /translation="MRKVLFLAIIVSLAICLVVLNCCSDINRRTPMDIEYFNLVKAIP
                     LGMHTDKIQYHSPSNTLYAMSIKSQEIYIFQDQERKNVLGGLGSSKVNFQYLADIAMG
                     SDNSLYVLDSSARIVKKFSSDGSPSGSIELKNSVQPKCLCVYQDQGLFIFDSASGEII
                     NYSGIDGTELNRFGRFQLQQVNSLFCNHDYLIAYDKANNVSNIFSVLGQFVKTEQGQV
                     VYDLYNNAICFNAGMLKCLSSSAYLPLAAENVFMCISNDLLALRIGEEIRIYKINYAR
                     VE"
     gene            complement(14016..15737)
                     /gene="recJ"
                     /locus_tag="PLW08_03050"
     CDS             complement(14016..15737)
                     /gene="recJ"
                     /locus_tag="PLW08_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPL51612.1"
                     /translation="MNKKWLIPSPPGKEINPEIEKLSAEFKLPRLVAELLYRKGIKTV
                     NSAQEFFKPSMDNLYDPYLFPDMEKAVQRILRAIDSKEKITIYGDYDVDGTTATALLY
                     LGLKRIGANIDFYIPHRMIDGYGLSLGSLDTLRENGSSLIISVDCGVNAIEEINTINS
                     MGMEIIITDHHNPKDELPPAFAIINPKLPGTKYPYEHLAGVGVAYKLLMAIYNNLGID
                     SAENKLKYMDLVAVGTIADIVPLTSENRIFASIGLQHLIEKKNLGLNALVQISGLNQK
                     KLDTTDIVFGIAPRINAAGRMGSASVSVELLISTDEAKSMELAEIIEHQNSLRQQEDQ
                     KTFQEACDIIEKKYKDLQQTSCMVVSSDDWHPGVIGIVASKLVEKYYRPVIMISFKDG
                     FGSGSGRSVADFDLFEALKQTEHNLHSFGGHKYAVGLTIYQEYLDRFENELTRFVSEN
                     LRLEQIQPPLQIDAEIELYNINNTLLDALEHFAPFGPDNTRPVFMTRNVTIAGYPYNV
                     GRNHLKLKVVKDGIYFDLIGYNLGDYLPLLKKNGKLNIAYTLEYNRFGNNLTIQGKLK
                     DLQILKD"
     gene            16150..17049
                     /locus_tag="PLW08_03055"
     CDS             16150..17049
                     /locus_tag="PLW08_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPL51613.1"
                     /translation="MLLASFLEAHNILFENRVLTKEQVYKELVDRLCSQYKLPISSSK
                     LLELIFKREEEVSCAYPTGIAIPHIRMDGFNDTLIAMAFLGNPLDYNGIKVNWVVLIL
                     TDKTSSKTYLNIVAALLKLSKDKEAMKALASAGDGHSVIHYLKKNEVEVKKDVIIADI
                     MVQNPIAVLPQNSLRELINLMSTHKVAGMPVVDETGKYVGEVNVLNLLEVGIPNYVMM
                     LDNLSFLSSYEPLENLFSQQDLLFVKDIMTTDEIFVRPEASIIETVFLMVSHNKRYLS
                     VVKEGKLVGLITAMDILRKVIAV"
     gene            17054..18331
                     /locus_tag="PLW08_03060"
     CDS             17054..18331
                     /locus_tag="PLW08_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HPL51614.1"
                     /translation="MLLMIIALLVFIVTYLCIITEWVNKMLAALIGGFVIIVLGVVDQ
                     SIAFSAIDWNVIFFLIGMMLTISVMRETGMFMYIAIKTAKIAKGSPLKIMVMMFIATA
                     VISAILGSVTTVMILVPIVLLIAEELKITPAPFIITMVIAANMGGAATMIGDPPNILI
                     ASATKYTFIDFFLNLTPAIVIIVIGSLGLIWLLYRGKMYVSNERRAKIMEYNDKNLIT
                     HPKLLWITLTVVGLMLLAFIFQKPLHLENATIAMAAGLILVFIGSRKKVETIILNDID
                     WVTIFFFIGLFMIVEGLVHTGFIDLLANGVMSITNGEPKTTSMVILWLSGILSAWIDN
                     IPFVAAMIPMIKSIGLHMQSAVQVQPLWWALSLGSCLGGNGTLVGASANIVAVGIANR
                     NGYKISFMDYTKIGILFALESMLLSSVYIWFRY"
     gene            complement(18654..22175)
                     /gene="nifJ"
                     /locus_tag="PLW08_03065"
     CDS             complement(18654..22175)
                     /gene="nifJ"
                     /locus_tag="PLW08_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPL51615.1"
                     /translation="MAKQTKATMDGNTAAAHIAYAFAEVAAIYPITPSSTMGELVDAW
                     ASEGRKNINNTKVDVIEMQSEAGAAGAVHGSLSAGAVTTTFTASQGLMLMLPNMHKIA
                     GEMLPTVFYVSARSLAAQSLSIFGDHSDVMSARNTGFALIACNSVQEVMDLGLVAQLA
                     TMKTRIPFLVFFDGFRTSHELQKIDVIDYETIAELNDPSLVEAFRKNALSPDKPRMKV
                     GQEAPDVYFQGRETSNLYYAKAPAIIAETMEQVAEKTGRHYQLFEYVGSPDAEDIIVA
                     MGSSCETIQETIEWLNAKRGMKLGLIKVHLYRPFCVQSFLSAIPASVKRIAVLDRTKE
                     PGSIGEPLYLDVVSALKNRPELYIIGGRYGLSSKEFTPSMVLAVYKHLAKNGFHGFTV
                     GIEDDVSHLSLPLEETINTVPEGTISCKFWGLGSDGTVGANKNSIKIIGDHTDMYVQG
                     YFQYDSKKSGGITRSHLRFGKTPIHSQYLVAQEDFVACHNQAFIGRFDLLGGIKENGV
                     FLLNSNWSRDEAFYNLTRPMQETIINKKIKFYNIDGLKIAEEVGLGGRINTTMQTAFF
                     LISGVLERNEAIALIKDSIRKTYARKGEKVVQMNLDAVDKVNEALVQIEVPETLPDKY
                     MPMKKLVPEDADDFILNVIEPIMREQGDQVKVSQMPLDGYVETGTAKLEKRKVAPSVP
                     HWLPENCIQCNQCSFVCPHSAIRTKLIKEEDLANKPASFNTLKATGAPGYEYKVQVYI
                     DDCQSCKVCVNECPKSALEMSPIEEERTKGEQVNYEYFETLPNDVLANFKETTVKGSQ
                     FKQPLLEFSGACAGCGETPYVKLLTQLFGDRMIIANATGCSSIWGGTFPTVPYCKNKD
                     GKGPAWANSLFEDNAEYGFGMRLAIRSHRKLLQKAMQELMDKNINEELKSAMQYSLDN
                     WDKVNEETKANADKIRALLPKAMETACEGCKILLKKVQELQDYLIEKSIWSIGGDGWA
                     YDIGFGGLDHTLALNQNLNILVLDTEVYSNTGGQASKSTPLGSIARFAEAGKNTNKKD
                     LGMMLMSYGYVYVASIAMGANKNQALQAIKEAEAYPGPSIVIAYAPCINHGFDMSLSQ
                     KHQKLAVDAGYWLLYRYNPLNKQEGKNPLTLDSKEPTVSVQEFLDSENRYAGLKNIFP
                     ERAKELNAGAAVFFKERYETYKKLAEG"
     gene            22642..22890
                     /gene="xseB"
                     /locus_tag="PLW08_03070"
     CDS             22642..22890
                     /gene="xseB"
                     /locus_tag="PLW08_03070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPL51616.1"
                     /translation="MKVSVDQEKINAMSFEESLKELEKIVDRLSTGETNLDELLSLYE
                     AGVAYLKHCQSRLSSAEAKIKILSEQLSSQQVTEGNNG"
     gene            22883..23773
                     /locus_tag="PLW08_03075"
     CDS             22883..23773
                     /locus_tag="PLW08_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPL51617.1"
                     /translation="MDRKILLKKDMKEKQELVNIILDRFLPRKDEYPKDLHKAMRYSV
                     FAGGKRLRPYLTMTAFEMFNSNIELITPVAAGIEMIHTYSLIHDDLPDIDNDDFRRGK
                     KSCHTIFGEGVALLAGDALLLSAFELITLAELDAKLRVQFVHELAKEVGIKGLIAGQM
                     VDIESEGKKVDKKTLHYIHENKTARLINLSLRFGALAGEAKEEELKILDEYGKLIGLV
                     FQIVDDLLDIEGTQEELGKTIGKDAESAKATFPAVYGIAESHKKAEELTEKAREILAP
                     LGEKALKLDILAEYLLGRKE"
     gene            23770..24204
                     /gene="dut"
                     /locus_tag="PLW08_03080"
     CDS             23770..24204
                     /gene="dut"
                     /locus_tag="PLW08_03080"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPL51618.1"
                     /translation="MNILVQKLSPTATIPQKMTENASGFDLFADISEPIILNPLEWRQ
                     ISTGIALEIPVGYEAQIRPRSGLSSTLGLSILNSPGTIDSDYRGEIKVILINLSPNPI
                     RIEPEMRIAQIVFCPVISPQLIEAQTLSESLRQDGGFGHTGQ"
     gene            24207..25118
                     /gene="nadA"
                     /locus_tag="PLW08_03085"
     CDS             24207..25118
                     /gene="nadA"
                     /locus_tag="PLW08_03085"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPL51619.1"
                     /translation="MKNNELIQEIKHLKKEKNALILAHNYQIVEIQDLADYRGDSLQL
                     SILAKEVKAPLIVFCGVKFMAETAAILNPESTILLPAIDAGCPMADMISLSQLKAFKQ
                     NYPGSPVVCYVNSSVEVKAESDVCCTSSNAVKILKSFSNEKPILFVPDRNLGSWAGKQ
                     SGKKVITWDGYCLVHQYGFSEEDVLTLKNEYPDYKLLVHPECPPQIIKYADLVISTGG
                     MVDWVKNNDKAIIATEIGLTEYLQHIYPEKSLIPLSPKAICKNMKKTTLENVYNALKY
                     NQYVISVEPNTAQKARKAIDRMLELSR"
     gene            25168..26046
                     /locus_tag="PLW08_03090"
     CDS             25168..26046
                     /locus_tag="PLW08_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51620.1"
                     /translation="MKTRFFLILLTLIILFGAWLYLSYNLGTNEMNKLEKLANLPIYA
                     YVADTTKVAPILTELKTVPGIKNVVHETALQAATELIQSYGLPLNEEMIKDYILPDII
                     TINLQPNRVSISNKPVIIDILRSHIPETDIDSQSSAYSLIIQELSNLDLYNIVFNVFI
                     AVLILLIFVFSRTALELNTLLHYKGIKYSALEKIRHQRQGLQHTLLMLIVPLPLCLLA
                     YFAYIYFKPLPQVIPYWVFLVQAGTVILGTMINYFILHSFEQQVALQENPIEVITPPE
                     MESNGNKEQENDTPFA"
     gene            26027..26590
                     /gene="pgsA"
                     /locus_tag="PLW08_03095"
     CDS             26027..26590
                     /gene="pgsA"
                     /locus_tag="PLW08_03095"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPL51621.1"
                     /translation="MTHHLPNILTVFRIILVPVFLYFLFLAKLPANILIALIIFIVAC
                     FTDYLDGMLARKMNIISDFGKLMDPLADKLIVLSALAGLCWLPPFQVSIVVFFIIFLR
                     ELVITILREIYQKKGMVVPADFFGKLKTVLQMAGIIVAFAFWVFINPVPPLVILIINV
                     WFWFVALITVLSGLNYIITIAKRSNYA"
     gene            26583..27644
                     /locus_tag="PLW08_03100"
     CDS             26583..27644
                     /locus_tag="PLW08_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPL51622.1"
                     /translation="MRKYMIFLLLLLVLFACSKEGGKIDRYTIYDKVIDHSKPLAMGD
                     DRDVYVFCDSLDWQGLKPFIGSAIEQQIIIVYPEQYFNLVMADIKDVKRLSKYKNLVF
                     IGHLESDLPVSAYMKRVLSQDFIKRVQQTGGELFTSENLNCCDQIVLFLLGKDAKTLQ
                     KIGALQAENIFSLLLKRFTNRQGYYAYQGKVIDPSFFDNYPFSLKIPDTYRLYSNDKV
                     GRFLCFLYRARLENREIPDKYISVYYEPMPENKVDLNWLIAKRQEIGKKYFEGDEFDP
                     ELVRKEPFTFKKHPGYRLLGAWKNMKYAIGGGFQSYGFWDEKTKTAYIVDSSVYFPAG
                     DKLPILVELYVISNSLEIK"
     gene            27641..29386
                     /locus_tag="PLW08_03105"
     CDS             27641..29386
                     /locus_tag="PLW08_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51623.1"
                     /translation="MKTILSCKQLLLWLAFFLLLLSACAGPQTTAQRTIIDNSQPNIP
                     SLYYYISASLKHYEGDFLTADNYYHLALEQDFRSYQIKKQILINSAYAFLSKQQDAEI
                     TVRQFDKARKEMIFDNDLLNAAYSVYSNANNEEGLAWVVSESVLYYPSTLSFLRKFYF
                     DYSKNKKSDENLLELALKYADNNPEDIILTAKMYTLVNPKRSIALLQEAYELEPKKET
                     DNLLSEITLQYLGKEEALKKFRTYSYPEDKDRMLYFLQMANRNRRLEVVNTLSPILLN
                     TGDSSLIGELAFSAYLEDKTDILQEISRFLLTKDADPLTDSKVVSFLFAEALFSEKLP
                     EPSLYLDYFNSAQDVQDVILYAMLKRSMQAKSLEKETNILFSEEFVSAVEKRLPENVF
                     SRYLKIASQIKSVEDEDFLQARAELCNYFLQKELGELEDWTFLLQYYQNEGREEEKIA
                     TLRKAVRKFPDNPLFLNDLGYSLLDYPQFIEEGGLLIQRAVALDPTNPYYQDSLAWFY
                     YLTANFSKAMEHITILMQMQDAPGEISYHIGMILLANQQKEEAIKYFQSALEDDQTPI
                     YQEKAKKALNELKTN"
BASE COUNT         9853 a         5317 c         5677 g         9579 t
ORIGIN      
        1 tgcctgttct tctactgccc gaggtggttt tgctcccaat tttaaaggaa acaatctggg
       61 ctttcgcata gtacgtaatg ttcattagag gaaaagggaa aattaagcca gagtaatata
      121 agcttatatc agacagtttg tagaaatata atttaatgac gagttaagat atatttatgt
      181 tattaagaaa gtagtttaat aaatcaatgt atttatgaaa ggagaaatag ctcagtttgt
      241 tcaggtaatc aactttcaaa tagatttata ataagtatat gactataata ttcaaaaatt
      301 gaattgtaca ttaaatcata atcaattatt attatagaaa aagacaatga agggatcaaa
      361 taataaacac atcattctaa ccagcaatat agaaaagagt aaaaaaagag attgacaaaa
      421 aacataggta aataaacaca ataaattcta aaatcccata taattaaagt tagattagaa
      481 tactaatatt ttactgaaaa atgaatatca cgagtttatt ctcatttgag gtatttaaga
      541 gtaaaaccta ctatataata ggtatattta gatgaaggag aggattaaaa tatggatttt
      601 ccaaaagaac taatagagac actcaggtta tataataacc aactttcaca agattttcaa
      661 aaaaagagaa tagaaactga agaagcccaa aaaaaagaaa agcttataac aaatctactt
      721 gaatatttaa acaaagggtg gccgatgcac ttcttaacaa tggctgatca acaatcagat
      781 ttgctaaaga gaatgaaaat aactaacccg tatgaaatag gaatcttaga atacgcatat
      841 agattttcaa aggaagaaac aacaaatata ttcaaaaggt atccaaattt attagaagaa
      901 aaatgtagag agtttaggat aacattagat aggaatagct ctcatccaaa atatagcgta
      961 aaaaatggtt tcttcaaaat tgaaatagtt gaagcaagaa gggttgcagt tttatcagat
     1021 tatgaaggga aacttataga attagctgca gatattgaac cagtagtaga agctcttcgc
     1081 aaagaatata taagagtttt tgacaggaaa tttcaaggta ttaaattcct aaagcaattg
     1141 aggtcaaatt ataaaatatt tattcagaaa aaacatcttc cagacggttc tagtgtacct
     1201 attaaagata ttgtaaagct aatgaagaaa aaaaataaaa agtttcggtt agatgaattt
     1261 attgctgatc tttcttcttt agttgaaaaa ggtccttttg agattgagga aagaacaata
     1321 gattttcaac aaaccaaaga cacaaaacaa ggtattctct taattgacaa ttcaattaga
     1381 gggtacattg gttatgtagt atttaaggag aaattatgag cgatcaaaat aatcttgcta
     1441 cgaatattgt taatgtatta agaatgggca tacctccaca aaagggtgtt gaacaatatt
     1501 ctgtaggaaa cgaaaaatta atcgaaggca taaaaaaatt ccatttaact gggatgtcaa
     1561 atcatggaat tattagattt gtttctggct cttggggttc agggaaaacc cactttttta
     1621 gattgcttag agaaattgct cttcaaaata actgtttagt ttcaaatgtg gaattagatg
     1681 tgaatagtgc tgctatgaat aaattccaaa gtatattttc tgcgattata agaatggttg
     1741 ctacgccttc atattatgaa gaagataaaa ttgacattgt tccttttgga atggtgatta
     1801 aagaatcctt agcatggtta gccactggaa gacatgaagc aaaatatgtt aatgtaccgt
     1861 atgaaaaata tcaaaaagca gttgaaatat taatggctga tcatagtatt gatattgact
     1921 ttaaaaaaat ggttgataat tattggaaaa cttttattat agatagtcct gatgaatcgg
     1981 cagtagaaaa aacacgcgga gaaattttac aatggttttg tggagagggc agtattggct
     2041 tttatagaaa gaattttaat ataaataagt tgattacaaa agaaaatgca aaaataatgc
     2101 ttcaatccct tgcaggtttt gttagattgt ctggatacag aggtttaata atcttatttg
     2161 atgaggcaga acaagctttt tctataatga gacaatcagt attaagagat gctcacaata
     2221 atttattatc gctgataaat aatattgaaa atgtgccggg tttattttta atctatgcaa
     2281 cagttcctga attttactct gatcccaagt atggcattat cagatatgga gctctttcag
     2341 gaagaattgg ccagccggat ccaaatcgtt tccctcgtgc tttagataaa atttggaatt
     2401 ttgatgcagt acaaactgaa ctcaacgatt atcaagaagt ggcattaaag ataaaaaata
     2461 tttatctaac tgcttatcct gaaacagaaa acattcttcc taatgatgta gaagtaaaga
     2521 aacgagttga agaactacat aaaaaacatt caccttatgc aggtattaaa ttttggagat
     2581 tactcgtttc gtctatggta gaagattttg ataatcatgc cgaaggtatt gttaagtcat
     2641 ctgataagtt atattacgat gttatggaga ggttaaaaaa cgagtaatct gtttgtaaat
     2701 aatcaacaag cacaagctat ccttgaatcg cttcgtaagg gtataccacc tgaaggattt
     2761 gttagaacct tcacagtagg aaggaaacat gaaattgatg accttaagaa aaggttagat
     2821 aaagatgatg ggactgttct tttggttaaa gcaaattatg gttccggaaa gacccattta
     2881 ttgaaattta tcaaagaaga ggcattagaa aaaaattatg ctgttagttt ggtaacaatt
     2941 gattcaaatt ctggtgtcag gtttaataga atggatcaga ttatgggtgc tgtttgcaga
     3001 aatatacaaa ttaattcaga aaagaatgag aaaggattag ctcctctatt taatttattt
     3061 gcaactaatt ttaaaaatga caagttgacc aattcttact ttaataaaat tagtaattat
     3121 ggtaaatggg attattctga aattttggac tcaaaagcaa tgtttattgc cttaagagct
     3181 tggacaacag gtcttcaatc agctcaagat actatcatag attggtttta taatccttcc
     3241 aattacagag aatcaaggaa acatctctat caatctttag ttttggaatt aagatcctat
     3301 tttcatgacc cacgtcctga atggaaattt tatgatgaca atgtatttgt aacgaataaa
     3361 aacggttacg aacaaagttg ggctgttctt agagatataa atactctatg taaacaatca
     3421 ggattaagag gttttattat tctttttgat gagtttgaag atgtaataac caatttgaat
     3481 aatgtgagat accaagaagg agcattttgg aacctatttc atttttatga gggaaagatg
     3541 tttcaaggca aaacattctt tgcagtgaca ccagattttg taaataaatg caaaagaaaa
     3601 ttgcatataa aggaaagatg ggattttgat tatgcaagat ttgataaatt accaatgtat
     3661 gaaatgagcc ctttacaatt attagaatta gaagaactat ctcttttaat ttttgatgtt
     3721 catagtattg cttatgaatg ggaaccggat acaattatgt tggattccta ttttaaatct
     3781 gttataaaaa aagctgccga agttccagtt caagatagag ttagaaatgt tattataaga
     3841 gttgttgaag aacttgataa tttatatcag gaattacatt gaattcaatt aacttattaa
     3901 atagtagagt ggcaactgct ttttatggta gatttaattt tataaacaat gcacaagaag
     3961 cagtaataga accattagtt aatgggcgca atattgtgtt atgttctggt actggttcag
     4021 gtaagacaga agcagttatg gctcctattg ttagcagata ttgggaagaa gctcttgaat
     4081 atgatgaatt atttataatc tatatttgtc ccactaaagc acttgttaat gacttagaaa
     4141 agagattaga acttcctcta agttatttag gattacgcat aggaattaga catggtgaca
     4201 gagatgactt aaaaaataat aaaattccac atgttttatt aaccacacca gaatcatttg
     4261 atgtcctcct gtttaagaag gaaacaagaa ttttgacagt aaaagcaatt atattggatg
     4321 aggttcatat tctatataac aatcaacgtg gattgcagtt atccattctt ctaaatagat
     4381 tgcaaatttt agttcaaaga aaaattcaat gggccgcatt atctgcaaca attggaaata
     4441 tgtcttttat acgtgatttt atcataggtt ctaattctca agctgatttt ttcagttatc
     4501 cttcagaaag aagaattgac tgttatataa cccatattaa aaatttatat atatttcaac
     4561 aattaattga gaagcttata gataatagta aattactgat atttacagat acaaggaaag
     4621 aatgcgagag acttgcgtcc tttttaaatg gttcagattt tctatcttat agactgtttg
     4681 ttcattattc ttctttatcg cgtggtattc gggtagaaac agaaaaaaaa tttgctgatt
     4741 catcttctgc agtatgtatt gctacaagta ctttagaatt aggtattgac atcggagata
     4801 ttgatgcaat tattatctgg ggtattccta ataatattga gtcatttatg cagagaattg
     4861 gtagaggaaa tagaaggtca caaaaaataa atgttatttg tcttgtgcca gatacatcag
     4921 aaactgtttt cttggatttg attaaatttt atatattaac tgaatgcgct attaatggta
     4981 atttgtccca agaaaatcct tataaacttt atggttcagt tgttcaacaa agcttaagta
     5041 taattgccgc aaatgaagga agattcgtaa aaagcgagga attaatgact gcacatagtc
     5101 atttacctta tataaataaa tttataatgg attcaattct tgctgaatta tgcgacaaag
     5161 agtttattct caaacacaat tttaaatata gttatgcagc ttatgaaaaa ttgtttcaaa
     5221 ttaaagattt aaggttaata tatggtaatt ttcctttagg aaccaaaatg gtgcgtttaa
     5281 tgagcgggaa gaaagaccta ggggaagttc ctgaaataaa tttagtaaga attaaacaag
     5341 gcagtatagt tagatttcaa ggtaaatact ggtgcgttaa aaaggtttcc accgatttaa
     5401 tttctcttga acccactagt aacaaaaaaa acagtataga ttttatttat cccagtagta
     5461 aaaaaagagt agattcatat attataaatc aggtatggtt tgcattacat aatgatatta
     5521 attataaaga gtattatgat tctaatctgt atacatacat tatttctaga caaaaattaa
     5581 ttcgtgataa tataccatta ttatcaatcc cttacatacg atataatgat aagataaaat
     5641 actatacatt tggtagtatg atagtaaata gggcaattgc attattcatg gactgtacag
     5701 aatttgaagc tgatgatatt tccctaacat tatcttctcc attatcttgg aatacgattc
     5761 ctgaaaatcc tatggaatat caaattatct tttctaaact ttttgataat ttagaacagc
     5821 aaactatatt tcagatgcaa ttacctcttg aattgcaact tgatgaatac ttacagtatt
     5881 ggctaaataa caagaccata gaagagattt tgaaacgctt aaagattagt attcccttag
     5941 agattaattt agaccttgta aaaaaaattg atttttatag cttatcataa tattatattt
     6001 tgatatatta gtagcatttt tattgcagaa taaaataact cttattatga taaaatgttt
     6061 aaaaaagaat gtgtgaggat atagggaatg aaaagaccgt ttattatttt gtttgtgctt
     6121 cttagcgcaa ttctttttgc tggtgagaga gggcttaccg ttaaagaaat tgaggtttcg
     6181 ggagaagtga tttcaatttc ctctcctgtg caatcactaa ttcaagtagc acctttaaga
     6241 aaggtagaaa aagttaaggc acttaaaaat tgggatacct tacaggaatt gaatttgaac
     6301 tttcgtcttc cggcaaaagg acaatatggt ttgcagttta tagacctttc tcagagtgtt
     6361 caaccctttg gtttcccggg atgtctttcg gaaacagcat ttcaggcagt taataaagct
     6421 cctgtttggg tgcagacgga attagcatcc attttagcag aactaatacc cgaaaaacaa
     6481 acactttggt caaatctaat tctttccgcg caagaccctt atgtggatga aatatgtttt
     6541 tgtattgcta attcttcgcc tcagtattta aattccgctt atgctttacc agagcttttt
     6601 acggaaaatg cgcaattgct ttattctatc gcttgtgaac tgccttatgt ggaaattatg
     6661 gatactggga ctgccatcag cggaggagat tattattcca caacccgtta ttggaagaaa
     6721 aatacttccg gtgaaatgca gcaaattacg gttcccagag aactttacta ctggtatatt
     6781 gttcatccca agctgacaga tgaaattcct gcttatatca atcccgctat aatagaaaac
     6841 aattcaacgc ataacaacaa tatagctcct ccaccaacgg gaaaattctg gcgtggatat
     6901 ctgtataatc ttcaggaaag tgattatccc gttctccgag atactttaat gcaatgtcag
     6961 acacttttca atcgtgacgg aacaggaaat gatgccatcc gcacaattca atggtggata
     7021 aatcaacaaa tgagctttac ttccaataat gaaagacctc atcagcccgt gagaattatt
     7081 actaaacaca ttggacgctg tggagaatat gccgatttaa cttcagccgt agcgcgttta
     7141 tccttaattc cctgcaccag tattttatcc tattctttag accatacctg gaatgaattt
     7201 tgggacgaaa attgggttgc ctgggaaccg gtaaatggtt acatagatat tcctttggtt
     7261 tatgaaaaca gctggggaac tgtctttggc agtgtttttg aaatccatcc cgatggactt
     7321 ttaacacctg taacggataa atattctgag ggggtttcta ccatcattat tcaggttaca
     7381 gatgccaatc aacatcctgt agacggagcc agagtaatgt tatatgttat gcaaaacagc
     7441 attcattttg attgtgagca atataccgat aataatggaa tcgctacttt cctggttgga
     7501 gacaacctgg aatattttac gagggtggaa accgatttcg gttcatttcc tgaaatagcc
     7561 ggaagttatc tgacattaac taacaattct gtagcggggg aaacatatca atatattata
     7621 acaattccca atgtaatgcc ggttccggag ttagaagaat tgcctcctcc tgctgatcct
     7681 gttcaggatt atcaatttgc cgtatcttac tatagtgaag gatattacat tacaggggtt
     7741 actttatggg atgatattta ttctttgggg gtgcgacctc ttcattataa gtatgtagca
     7801 actcccggag agctatcctt tatggtaatg gatagtgata atattcttat ttggcagctg
     7861 gataattttg gctccggcta cagttattca ggtactgcat ccgagggaac agcatctttc
     7921 agtatccctc ttggctcgga ttggtatgcc tttatggata attcattgca ccatcgtaat
     7981 gctgtaaaaa tcaatggagg actgcttttt caacataccg gaacggctgt tcaggatgaa
     8041 acaataccta atgttttact acaatattcc atctatccta atccttttaa caaaacaacc
     8101 tgcttatccc tgaaggcaga taaagatttt aaggcagata tttctatcct gaacctgaaa
     8161 ggacaggtag ttaaatcctg gcaaaataca ttcattaaac agggaaactc cgatttttac
     8221 tgggatggaa aagataataa gggccgggat ataagctccg gagtttattt ctggaaagtc
     8281 caaagcggag aaaccgcttt taccaacaag atactttacc tgaaatagat aagttatttc
     8341 cggaatgata attcaattat ccctatagaa aaaataaggt tgagataaag ttccaagggg
     8401 cgacacaatc atagctcggt tgtatgcgta aaagcaagtg gttgatgata attggggaag
     8461 caggtgaaaa ttgtaagtca aaaaatctct gccacgaaat aatgggtaaa ccacaaatat
     8521 gttttatcat ttccattttg aaaattcctt tgtccttccg gtgaaagcag gaaaccaact
     8581 tatttgcata tagctaacta aattttaagt atggaatttg gggatttttg taagggagca
     8641 ttgctcactc attgcttacc cattactaat gccattagca atgggtaagc aatgggtaag
     8701 caatgctatt agggaagaga atataatcta ttgtgttatg gtgaattagc aaaataatta
     8761 ggtatgggtc tttgtgctgg aaagtttcag gaaaatatca aaagtcgtca cgagtcataa
     8821 gcgccaaaaa agttatttca aaggtcaaag attagaaggt gctaatgact cttgacttaa
     8881 atttttccga ggagcttaca cacccatcgc tatcattgta tcgctctgcc ggctttggaa
     8941 aaatctcaag cttaaaaacc ctctcaaccc tcttaacctt ttttctccag ggatgtaatt
     9001 tcgctcatcc atttgagaac ggcagtttgg aagatttctt tcagctgaaa ataagcaggc
     9061 aggttcagtt ctcctgtcca ttcatccata aaaatctttt tgaattcaat agcaaggcag
     9121 agaactttgt tgccgaaggt ctgatttaca caacgggaaa aatgtcctcc gctaaatttt
     9181 atatcgcagc ggcagtccaa ttttatgttt tgaaaagggt taccatccag tagcacacgc
     9241 aaattttcta caacaggata aacactttcg ggaaggttat ttctgcccaa aataagatcg
     9301 ggattttggt tttgagggtc gggagcaaca tcaggaccgc ctcttctatg attatagcta
     9361 tgcaggtcta aaataattag ggaggaatga attttcagca agcgttcaat ttgatagcag
     9421 ataatctgat accagtcagc gtatgcctta agcaattttg catacaggtt atccgcgata
     9481 ggcattttac gaacctgcaa gccccaagca tcttccggtt tttgatagac agctttttcg
     9541 ggaggacgat tcaaatcaac ggcaaaacgg ctggtttgca caataattct attgggaaaa
     9601 cattccgcaa agccattggt ataagggtct tcttcgcgta atcttgtgtc tgcatcaatt
     9661 ccacagttct gtaaaagttc tggtggcata taattgccat tatgaatagc aaaagccaat
     9721 aacgggaatt tattatcccc caaatagata tcactataaa tcattgatca agctgttcca
     9781 gatattgcat taaaccttct aaaatgccca aatactgttt aatatcttcc aatgtagctt
     9841 catcgccgga aacccagtca agcataggtt caaattttac attggtaata atgttatttt
     9901 ttgccaaaat ttcctcaagt ttaaggggaa caacaccatc atacagataa caaatacctt
     9961 tcaacacggg aataatattg ctcaaagcta taaacagaag gtcggacaat tgtttgggag
    10021 tatttttccg ttccaaaaca gacaaacgga taagcagcca tttgcctttt atttcgcgtt
    10081 ccatttgtaa acgcagatca tcaggatcaa attgaagatt ctttaaaata tcctctttaa
    10141 cgaaaaggtt ttgataattg ctggtaatga tatccagaaa ttccagggga taggaatcta
    10201 aagaactttc tacaaacttg cggtttacca gtaaaggaaa acgcaattga cgctgatggg
    10261 aatactttct taaagaatct aaagattcca gaagatcggg agggtctttt tccagaataa
    10321 ttatgcattc cggtttaaaa tcatcataaa tagcaacttg taaaaggtaa tcctgaatac
    10381 cagataaagt attcattact tcagaaaaat cgcgggtcat aatgcctcct ttttttaagc
    10441 gttatatttg gttaaataag cattggcaca ggcgcgagcc aaatttcgta ttctggctat
    10501 gtaagatgct ctttccgtaa cacttatcac tcctctggct tccagtaagt taaaagtgtg
    10561 agaacatttt aacaacatat cataagcagg ataaattagt tgtttaccta aaagggcatt
    10621 gcattccttt tcataatcct gaaaaagctg gaacaacagg tttgtatctg cctcttcaaa
    10681 gttataggca ctatattctt tttcgcgctc atagaaaagt tccgcataac caatattttc
    10741 attccaggca agcaaacggt aatcatccac attttgaata tacattgcta tgcgctccag
    10801 tccataagtt agttctacac taacgggaaa aacatctatg ctggcgacct gttgaaagta
    10861 tgtaaattga gaaatttcca ttccatccaa ccagacctcc caacccaaac cccaagctcc
    10921 taaagttggt gattcccaat cgtcttccac aaaacggata tcgtgcattg aaatatcaat
    10981 tccaatagcg gataagcttt ttaagtataa attttgaata tcatccggag ttggcttgat
    11041 aatcacttga aactgataat aatgttgaaa acgattggga ttttctccat aacgaccgtc
    11101 tttgggtctt ctacaaggtt gcggataagc aatggcacaa ggtttttttc ctaaagtgcc
    11161 aaaaaaggta gaaggatgaa aagttcctgc tcccatttcc aaatcgtagg gttgaattaa
    11221 acaacaaccc ttttcagccc aataattttg cagagttagt atcagatctt gaaagttcat
    11281 tttttatcct gattatagtt tttacaggtt tgctgaataa gcgtcaggtc tttttttatg
    11341 tcctgcagta tttccgttat tccagttttc atttctgaat ccttttttgg ggaagataga
    11401 gaatcgtcaa gtgtctcttt tgttttagtt tgaattttgg ttctttcctg cttaattatc
    11461 tttctggcac cttcaatggt atagcgctga ttatagagca ggtcgtaaat tctacgcagc
    11521 aaaagaacct gctgttcatt gtattttctg atgcgccctt cactctttat ggcattcagc
    11581 ccgggaaatt cagtttccca atagcgaata atatagggct taacacctaa aagattgcta
    11641 acttcgccta tagtgtaata atattttttc ataagacctc acctttttgt ttctttttag
    11701 tgaagctgaa gaggaaaagt cctcccgcca gaattacaaa aatccacgga taccaataaa
    11761 tataacgata aacgggtata cgggaacaac gataaagagg tgcttgcaaa tttgtgattt
    11821 caaagagacc cgctttggca attatgcggc caaaagggtc tacaattaaa gaaattccgg
    11881 tattagcact acggtaaatt tgaatccggt tttccactgc ccgaaatttt gccataatgg
    11941 aatggagcca gggaccgtag gaagttccaa accaggcatc attagtaata tttaccaaaa
    12001 aatcggcttt ttttaattta cctgttgttt tgtctttcct gattgccatc tgctgatgaa
    12061 attcagcaaa agcaagctca taacatattg aaggggaaaa ggttaaacta tccgtttggt
    12121 aataaaccgg tccgttgcca aattcccagt ttgcctgtcc aaactgcaat ttccataaaa
    12181 aagggaaaat atttagccac agcattcttt caccaactgg aaccagaata tttttataat
    12241 acaattccga aattgagcca tcgggatgaa aaagagccgc tgcattgtaa tataattcac
    12301 tgttaatatg attttcagga gctaaagtaa aatgcggaaa gccggtaaaa atttccagtt
    12361 catatttttg catcaggtct ttcaaaaagt ttcgtgcccg ataatcatac attaagtaaa
    12421 ccggcattgc ggcttccgga aaaattatta gtttggtgga atcctttttt gcctgagcac
    12481 aaagagcatc ataacgatgt aatatttgct gataggcact ctcatcccat ttttcgtcct
    12541 gaggaattga gggttgcata acggttattt tggggtcttg tttttgaacg ggcaaatagt
    12601 ttaaacaata tataccataa ccaatccaaa gaataaagat gccggctaca gataatagac
    12661 attgcgtctt ctttttaagg agaacttcat aaagcaaaac attgatgagc aaaataagca
    12721 aagaaagtag aattacccct cccaaatcac ctgcctgaat taaaacagta tagtccgata
    12781 gcgaataggc attattaaac cacggaaatc ttgtttctcc aaaattttgc aaatattcaa
    12841 agcttaacat cacagcaata aaattagggt aaaaaagacg gggataaaat ctacctatcc
    12901 tgctaattgc ataaaagcaa agccagtaaa ataagaaata gacaatagcg ataccgatca
    12961 aaccaaaaaa agtaactccc gctatccaat acattacaac accgatataa acaacagaaa
    13021 ataaaagtcc tgcctgccat aatttcactt ttctccgctc taaaacatag agcaaaggaa
    13081 tccaaccaaa gaaaaccatc caaccacaat gtattggtaa acgcgataag gcaagcatta
    13141 aagccgaaag taaaacccaa agtaaatcca tcacttattc cacccgcgca tagtttattt
    13201 tataaatacg gatttcttct cctattctta aagctaataa atcgttactg atacacataa
    13261 atacattttc cgcagctaaa ggtaaatagg cagaactgga taaacatttc agcattcctg
    13321 cattaaaaca gatagcgtta ttgtataaat cataaaccac ctgtccctgt tccgtcttta
    13381 caaattgacc cagcacggaa aatatgttgc ttacattatt tgccttgtca taagcaatca
    13441 gataatcgtg attgcaaaaa agagaattta cctgctgaag ctgaaatctg ccaaaccgat
    13501 tcagttcggt tccgtcaatt ccagaatagt tgataatttc tccgctggca gaatcaaaaa
    13561 taaataaacc ctggtcttga tatacacaaa ggcatttggg ttgcacactg tttttaagct
    13621 caatactgcc ggaaggactg ccgtcggaac tgaatttttt cacaatgcga gcagatgagt
    13681 ccaacacata taaagaatta tcactaccca tagcaatatc tgccaggtat tgaaaattaa
    13741 ctttggaact gcctaaacca ccaagcacat ttttccgctc ttgatcctgg aaaatataaa
    13801 tttcctgact tttaatgctc attgcataaa gcgtattgct gggggaatga tactgaattt
    13861 tatctgtatg cattcccaaa gggatagctt taaccaaatt aaaatattca atatccattg
    13921 gagttcttct gttgatgtcg gaacagcaat ttagcacgac caggcagatt gctaaagaaa
    13981 caatgatggc taaaaataaa accttccgca tagttctaat cctttagaat ttgtaaatcc
    14041 ttaagcttgc cttgaatggt taaattattc ccaaaacggt tatattcaag ggtgtaggca
    14101 atattcagtt tgccattctt tttcagcaga ggtagataat ctcccaaatt ataaccaatt
    14161 aaatcaaagt aaatgccatc tttaactact ttcagtttca gatgatttct tcctacatta
    14221 taaggataac ctgctatagt tacattgcgg gtcataaaaa caggacgcgt attatcagga
    14281 ccaaaaggag caaaatgctc caaagcatca agcaaggtat tgtttatgtt gtaaagctca
    14341 atttcggcat ctatctgtaa aggtggttga atttgttcca ggcgtaagtt ttcggaaaca
    14401 aacctggtta attcgttttc aaaacggtct aaatattcct gataaatagt taaacccaca
    14461 gcatatttat gacctccgaa actatgcaaa ttatgctctg tctgcttcaa tgcttcaaaa
    14521 agatcaaaat cggcaacact gcgtccggaa ccgctgccaa aaccgtcttt gaaggaaatc
    14581 attatcaccg gacgataata tttctctacc agcttagaag caacaattcc gatcactcct
    14641 ggatgccagt catcggaaga aacgaccata caggatgtct gttgcaagtc cttatatttc
    14701 ttttcaataa tgtcacatgc ttcttgaaat gttttctggt cttcctgttg ccgtaaagaa
    14761 ttctgatgtt caataatttc tgctagttcc atactctttg cttcatcggt tgaaattaaa
    14821 agttctacgg aaacggaagc gcttcccatt ctgccggcag catttattct gggtgcaatg
    14881 ccaaaaacga tatctgtggt atccagtttt ttctgattca acccggaaat ttggactaaa
    14941 gcatttaagc ccaggttctt tttctctatt aagtgctgaa gcccaatgga agcaaaaata
    15001 cggttttccg aagtgagggg aacaatatcg gcaattgtgc caactgccac aagatccata
    15061 tacttaagct tgttttccgc actgtcaata cccaagttat tgtagattgc catcagcagt
    15121 ttataggcaa ctccaactcc ggcaagatgt tcataagggt attttgttcc tgggagtttg
    15181 ggattgataa tagcaaaagc aggaggcaat tcatctttgg gattgtgatg atcagtaata
    15241 ataatttcca ttcccataga gtttatagta ttgatttctt caatggcatt cactccacaa
    15301 tcaacactga taataagtga agaaccattc tctcttaaag tatccaaact gccaagcgat
    15361 aaaccgtaac catctatcat ccgatgagga atgtaaaaat caatattggc accgatgcgt
    15421 ttcaaaccta aatacaataa agcggtagca gttgtgccat ctacatcgta atcaccgtaa
    15481 atagttattt tttctttgct gtctatagcg cgtaaaatac gctgaactgc cttttccata
    15541 tcgggaaaga gataggggtc atataaatta tccattgagg gcttaaaaaa ctcctgcgca
    15601 gaatttacgg tctttatccc tttacggtat aataattcag ctaccaaacg cggaagcttg
    15661 aattcggcac ttaacttttc aatttcaggg tttatttctt tacccggtgg agatggtatt
    15721 aaccattttt tgttcatcat tctgccttta tatattttct ctatttagat gtggaactaa
    15781 cataacctta ataggttaca gcatattctt aagagaactg tatgctttac acattctaaa
    15841 ctctataatt atagagcgct aaatcctgta aagcattatt ttcattccgg aaatatgctg
    15901 tctttttaga tgaataactt aataaattga aaagtgtaaa ggtgtaaaag gggaaaggtg
    15961 aaacacgagt taaacataga taacaagcaa aaaatctgta tgaagccata ttccaaagct
    16021 gtttcaacag atcatactcc attttccatt cattccatct taccctatcg ccatcttgcc
    16081 aaaataaata ttgaccgaaa aagagagaac attaaaaagg agacaaaatt acctgttaag
    16141 aggtgttaaa tgttattagc cagctttctg gaagcgcata atattctgtt tgaaaacagg
    16201 gttttaacaa aagaacaggt ctataaggaa ttggtagacc gattgtgcag tcaatataaa
    16261 ttgccgatta gcagttccaa acttttagag ctgatattta aacgcgagga agaggtctcc
    16321 tgtgcttatc caacaggtat agctattcct catattcgta tggacggctt taatgatact
    16381 ttgatagcaa tggctttttt ggggaatccc cttgattata atggaataaa agttaactgg
    16441 gtagttctta tattaaccga taaaacaagt tccaagacct acctgaatat tgttgctgcc
    16501 ttgttgaaac tttccaaaga taaagaagca atgaaagctt tggcatccgc gggagatgga
    16561 catagcgtta ttcattattt gaaaaagaat gaagtggaag tgaaaaaaga tgtaattatt
    16621 gctgatataa tggtgcaaaa tccaattgca gttttgcccc aaaacagttt gcgggaacta
    16681 atcaatctga tgagtacaca taaagttgca ggaatgcctg tagttgatga aacaggaaaa
    16741 tatgtaggtg aagtgaatgt tcttaatttg ttggaagtag gaattccgaa ttatgtaatg
    16801 atgttggata atttaagttt tctttcttct tatgaaccac tggaaaatct tttcagccaa
    16861 caggatttgc tttttgtgaa agatattatg actacagacg aaatatttgt gcgtcctgag
    16921 gcatctataa ttgaaactgt gtttttaatg gttagccata ataagcgtta tctttctgta
    16981 gtgaaggagg gaaaacttgt aggtctgatt acggcaatgg atatactaag aaaggttata
    17041 gcggtataat aaaatgctgt taatgattat tgctttattg gtctttattg taacttatct
    17101 atgcatcatt acggagtggg tcaataaaat gctcgctgct ctgataggtg gttttgtaat
    17161 tattgtttta ggagttgtag atcagtccat tgctttttcc gcaattgact ggaatgtaat
    17221 ctttttcctg attggaatga tgcttaccat ttccgtgatg cgagaaacgg gaatgtttat
    17281 gtatattgct attaaaacgg caaaaattgc caagggcagt cccttgaaaa ttatggtaat
    17341 gatgtttata gcaacggcgg ttatttctgc cattttaggt agtgttacca ctgtgatgat
    17401 tttagttcca atcgttttgc tgattgcaga agaactgaaa ataactccag caccctttat
    17461 tataactatg gttattgctg ccaatatggg tggagcagca acgatgattg gtgacccgcc
    17521 aaatattctt attgccagtg ctactaaata tactttcatt gattttttcc tgaatctaac
    17581 tcctgcgatt gtgattattg taattggcag tttaggttta atatggcttt tatacagagg
    17641 gaaaatgtat gttagcaatg aaaggcgtgc caaaataatg gaatataacg ataagaacct
    17701 gataactcac ccgaaactat tgtggattac tcttacagta gttggtttga tgttgctggc
    17761 atttattttt caaaagccgt tacatttgga aaatgctaca attgcaatgg cagcaggtct
    17821 tattttagta tttataggta gtcgtaaaaa ggtggaaaca attattctca atgatattga
    17881 ttgggttacc atctttttct tcattggcct ttttatgatt gtagagggtt tggtgcatac
    17941 tggctttatt gatctcttag ctaatggtgt gatgtctatc accaatggag agccaaaaac
    18001 aacttctatg gtcattcttt ggctttcggg cattctttct gcctggatag ataacattcc
    18061 ctttgtagca gcaatgattc cgatgattaa aagtattggc ttacatatgc aaagtgcagt
    18121 tcaagttcaa cctctttggt gggctttatc tttaggatcc tgtttagggg gaaatggaac
    18181 tttagttggt gcttctgcta atattgttgc tgtaggaata gctaatcgta atggatataa
    18241 aatatccttt atggattata ctaagattgg gattcttttt gctctggagt caatgcttct
    18301 ctcgtctgta tatatttggt tcagatatta atcctggaaa agttcgagga ttatggctct
    18361 cacagatagc actgataaca cagattaaat tttatagaga ttttttacag gactgacggc
    18421 ttggtttacg cagattttat tataatatca tttcttgaat gatatttccc ttgtccttct
    18481 tgagaaagca ggaatatagg gtatttttcc aaatatcaat gtattttcaa tataggaact
    18541 atataaaggg tattttcggg tcgtcacgag tcatatacgc cgacaaagtt atcgtctggg
    18601 acaagtttgt ataggcgtaa atgactcctg acttaccata gttgaccggg aggtcaacct
    18661 tccgcaagct ttttatatgt ttcgtatctt tccttaaaga acacagcagc gcctgcattc
    18721 aattctttag ctctttccgg aaagatgttt ttgagtccgg catagcgatt ttcggaatcc
    18781 agaaattcct gcactgaaac cgttggctct ttactatcca gggttagagg atttttacct
    18841 tcctgtttat ttaacggatt ataacgatag agaagccaat aacctgcatc cacagccaat
    18901 ttttgatgct tttggctcaa actcatatca aagccatgat tgatgcaagg agcataagca
    18961 attacaattg agggacccgg atatgcctct gcttctttaa ttgcctgcag tgcctgattt
    19021 ttattggcac ccatagcaat ggaagcaaca taaacataac catagctcat tagcatcatt
    19081 ccgaggtctt ttttgttggt attttttccg gcttccgcaa accgcgcaat ggatcctaag
    19141 ggagttgatt tacttgcctg tcctcctgta ttggaataga cctcggtatc caaaacaagg
    19201 atattgaggt tctgatttag agccaaagta tgatccaatc ctccaaaacc gatatcataa
    19261 gcccaaccgt caccaccaat tgaccaaatt gatttctcaa tgaggtaatc ctgaagttcc
    19321 tgaacctttt ttaggaggat tttacagccc tcacaggcag tttccattgc ttttggaagc
    19381 agagctctaa ttttatcggc attggctttt gtttcttcat ttactttatc ccaattatct
    19441 aaggaatatt gcattgcgga ttttagttct tcatttatat ttttatccat cagttcctgc
    19501 attgcctttt gcagaagttt acggtgagaa cgaattgcca aacgcattcc aaaaccgtat
    19561 tcagcattat cttcaaataa gctgtttgcc caggcaggac ctttaccatc tttgttttta
    19621 caatagggaa cagtaggaaa agttccaccc caaatgctgg aacaaccggt agcattggca
    19681 ataatcattc tatcaccaaa gagttgagtt aagagtttca cataaggtgt ttcaccacaa
    19741 ccggcacaag cacctgagaa ttccagcagg ggttgtttaa actgactgcc tttcacggtt
    19801 gtttctttaa agttggcgag aacatcattg ggcaatgttt caaagtattc gtaattaact
    19861 tgttcgcctt tagttctttc ttcttcaata gggctcatct ctaatgcgga tttaggacat
    19921 tcattcacgc agaccttaca gctttggcaa tcgtcaatat aaacctgaac tttatattca
    19981 taacctggag cgccagttgc ttttaaggta ttaaaactgg ctggcttatt ggcaaggtct
    20041 tcttctttaa tcaattttgt ccggatagcg gaatggggac agacaaaaga acactgatta
    20101 cattgaatac aattttccgg aagccaatgg ggaactgaag gggctacttt tctcttttcc
    20161 agtttagcag ttccggtttc aacatagcca tctaaaggca tttggctaac ttttacctga
    20221 tcaccttgtt ctcgcataat gggttcaatc acatttaaga taaagtcatc ggcatcttcc
    20281 ggaactaatt tcttcattgg catatattta tcgggcaggg tttcaggaac ttcaatttgc
    20341 accaatgctt cgttaacttt gtccacagcg tccaaattca tttgaacaac cttttcgcct
    20401 ttacgagcgt aggtctttct aatggaatcc ttaattaagg caatagcttc attgcgttcc
    20461 aaaacaccgg aaataagaaa gaaagctgtt tgcattgtag tatttattct tccgccaaga
    20521 ccaacttctt ctgcaatctt taagccatct atattgtaaa actttatctt tttgttaatg
    20581 atggtttcct gcatcggacg ggttaaatta tagaatgctt catctctact ccagttggaa
    20641 ttcagcagaa aaaccccatt ttcttttatt ccgccaagca aatcaaatct gccaataaat
    20701 gcctgattgt ggcaggcaac aaaatcctct tgagctacca gatattggct gtgaatgggg
    20761 gttttaccaa aacgcaaatg gctacgtgtt ataccaccgc tttttttgct gtcatattga
    20821 aaataaccct gaacatacat atcggtatgg tctccgatga ttttgatgct gtttttgttt
    20881 gcaccaacag taccatcgga acctaaaccc cagaatttgc aggaaatagt gccttcagga
    20941 acagtattga ttgtttcttc aagcggtaaa gaaaggtgag aaacatcatc ctcaatgcct
    21001 actgtaaaac cgtgaaagcc atttttagcc aagtgtttat aaacggcaag caccattgaa
    21061 ggagtaaatt ccttactgga taaaccgtat ctgccaccaa ttatgtatag ttcaggacgg
    21121 tttttaagag cagaaaccac atccagataa aggggttcac caattgaacc gggttctttg
    21181 gttctgtcta aaacggcaat tcttttcacg ctggcaggga ttgccgataa aaagctttgc
    21241 acacagaaag gacggtataa atggactttt atcagtccca atttcattcc gcgcttggca
    21301 tttaaccatt caatcgtttc ctgaatggtt tcacagctac ttcccatagc tacaataata
    21361 tcttctgcat cgggagaacc gacatattca aaaagctgat aatggcgtcc tgttttttct
    21421 gctacctgct ccattgtttc tgcaattata gcaggtgcct tggcataata taaattactg
    21481 gtttctctac cttgaaaata tacatcgggt gcttcctgac caaccttcat tcgcggttta
    21541 tcaggactta aagcattttt ccgaaatgct tccactaaag aggggtcatt cagttctgct
    21601 attgtttcat aatcaataac atcaattttc tgcagttcat gactggttcg gaaaccatca
    21661 aaaaacacca gaaaaggaat ccgggttttc attgtagcaa gttgagccac taaacccaaa
    21721 tccatcactt cttgcacact attacaggca attaaggcaa aaccggtatt acgagcactc
    21781 attacatcgc tgtgatcccc gaaaatagaa agggattgag ctgccaaaga ccttgccgaa
    21841 acataaaaaa ccgtaggtaa catctcaccg gctattttat gcatattcgg cagcattaac
    21901 atcaaaccct gagaagcagt aaaagttgta gttacagcac ctgcggaaag tgaaccatga
    21961 acagcacctg cagctccggc ttcactttgc atttcaataa catcaacctt ggtgttatta
    22021 atattttttc tgccttcgga agcccaggca tcaacaagtt cacccattgt ggaagaaggc
    22081 gtaataggat aaatagctgc tacttcggca aaagcataag caatgtgagc cgcagcagta
    22141 ttaccatcca ttgtggcttt ggtctgtttc gccattgtat aactccttat atttcatatt
    22201 tatatgtttg aataacagtt acagggacaa gatttgagaa agcatttttc gtcaatctaa
    22261 atcataaagt tagcgcacag atgtcataga tgtctctcac agatgtcacg gattttatgg
    22321 attttattta caaaaacagc gctaaatgtg tagttggagg atgctgttcc tccgcatttt
    22381 ttcaaccacc gaaaacggca agaacaccaa acgcattaag aattatttat gttatccgct
    22441 ttatattcta actatattct ttttctatca gttatccaat tgttcccaag agcattgctt
    22501 actcattgct cacccattac ggatggcatt agcaatgagt aagcaatgag taagcaatgc
    22561 tattatggga gaaaaaaatt tgtgattgac aaaataacat tacttcggta aaaggaaata
    22621 attataaatt atataggtga ggtgaaagtg agtgtagacc aggaaaaaat taacgcaatg
    22681 agctttgaag aatccttaaa ggaattggaa aaaattgtag accgcttatc cacaggcgaa
    22741 acaaatcttg atgaattact ttcgttgtat gaagcgggag ttgcttattt aaaacactgt
    22801 caaagtcgtt taagttctgc cgaggcaaaa attaaaatcc tcagcgaaca gctttcctcg
    22861 caacaggtaa cagaaggcaa taatggatag aaaaattctc ctgaagaagg atatgaaaga
    22921 aaagcaggag ctggtaaata tcattttaga ccgcttcctc ccgcgcaaag atgaatatcc
    22981 taaagacctt cataaggcaa tgcgttatag tgttttcgct ggtggcaaac gcttgagacc
    23041 ctatttaaca atgactgcct ttgaaatgtt taattccaat attgaactga ttactcccgt
    23101 tgcagcaggc attgaaatga ttcatactta ttcgctgatt catgatgatt tgccggatat
    23161 tgacaatgac gacttcaggc ggggtaaaaa atcctgccat actatttttg gagaaggagt
    23221 tgctttgctg gcaggagatg ctttactctt aagtgctttt gaactaatca ctttggctga
    23281 actggatgca aaattgcgtg tccaatttgt tcacgaatta gctaaagaag ttggtatcaa
    23341 aggtttaatt gcaggtcaga tggtagatat tgaatcagaa ggcaaaaaag tggataaaaa
    23401 aaccctacac tatattcacg aaaacaaaac tgctcgcctg ataaatctaa gtttgcgttt
    23461 tggagctctc gctggtgaag ccaaagaaga ggaattgaaa attttggatg aatacggaaa
    23521 gttgattgga ctggtttttc agattgtgga tgatcttctg gatattgaag gaacgcaaga
    23581 agaacttggc aaaactatcg gcaaggatgc tgaaagtgcc aaagctactt ttcctgctgt
    23641 ttacggtatt gcggaatctc ataaaaaggc agaagaactt acagaaaagg caagagaaat
    23701 tcttgctccc ttaggggaaa aagcattaaa attggacatt ctggctgaat atctattagg
    23761 taggaaagaa tgaatattct cgtgcaaaaa ctatctccga ctgctactat cccgcaaaag
    23821 atgacagaaa acgcttctgg ctttgatctt tttgctgata ttagcgaacc aattattcta
    23881 aatcccttgg aatggagaca aatttccaca ggcatagctt tagaaattcc cgtaggttat
    23941 gaagctcaaa taagacctcg cagcggttta tcttccactt tgggactaag cattttaaac
    24001 agtcccggca ctattgattc cgattatcgc ggtgaaataa aagtgatttt gataaacctt
    24061 agccccaatc ctattcggat tgaacctgaa atgcggattg cacaaattgt cttttgccct
    24121 gtaatttctc ctcagctaat tgaagctcaa actctttctg aaagcttaag gcaagacggg
    24181 ggttttggtc atactggaca ataaaaatga aaaataatga actgattcag gaaataaaac
    24241 acctgaagaa agaaaaaaat gctctgattt tggctcataa ctaccaaatt gtagaaattc
    24301 aagaccttgc tgattaccgg ggtgattctt tacaactctc tattttggcg aaagaagtta
    24361 aagcaccttt gattgttttc tgcggagtga aatttatggc agaaaccgct gccattttaa
    24421 atcccgaatc tacaatttta cttcctgcaa ttgatgccgg ctgtccgatg gcagatatga
    24481 taagtttaag ccagctgaaa gcatttaaac aaaattatcc tggttccccg gttgtgtgct
    24541 atgtaaattc ttctgtggaa gtgaaagccg aaagtgatgt ttgctgcaca tcctccaatg
    24601 ccgttaaaat cctgaagtcc tttagcaatg aaaagccgat tttatttgtt cctgatcgca
    24661 atttaggttc ctgggcaggc aaacaaagcg ggaaaaaagt aattacctgg gatggctact
    24721 gtcttgttca tcaatatggt ttcagtgaag aagatgtttt aactctcaaa aacgaatatc
    24781 cggattataa acttttagtg catccggaat gccctccgca gattattaaa tatgccgatt
    24841 tggtaatttc aacaggggga atggtggatt gggtgaaaaa taatgacaaa gccataatcg
    24901 ctacggaaat tgggctgacg gaatatctgc aacatatcta tccggaaaaa tcgcttattc
    24961 ctctttctcc caaagcaatt tgtaaaaata tgaaaaagac cactctggaa aatgtttata
    25021 acgctctaaa atataatcag tatgttataa gcgttgaacc aaacacggca caaaaggcaa
    25081 gaaaagctat agacaggatg ctggaacttt caaggtaagt aaaatgcttt ttctattcaa
    25141 cctctattct aaaaaaagga taacgctatg aaaacccgct ttttcctgat tcttttaacc
    25201 ctgataattt tattcggtgc ttggctttac ctaagctaca atttgggcac aaacgaaatg
    25261 aataaactgg aaaaattggc aaatctgcct atctatgctt atgttgcaga tacaacaaag
    25321 gtagctccaa ttttaacaga gctgaaaaca gttccgggaa ttaaaaatgt ggtgcatgaa
    25381 acagctttgc aggcagctac ggaactgatt cagtcctacg gtcttccttt gaatgaagag
    25441 atgattaagg attacatttt acctgatata ataacaataa atttgcagcc aaaccgtgtt
    25501 tccataagca ataaacctgt tattattgat atattacgct ctcatattcc ggaaacagat
    25561 atagacagtc agtccagtgc atatagttta ataattcaag aacttagtaa tttagaccta
    25621 tataatattg tgttcaatgt atttattgcc gtgctgattt tgctgatttt tgtgttttcc
    25681 cgcactgctt tggaacttaa cactctgttg cattacaaag ggattaaata tagtgctttg
    25741 gaaaaaatcc gacatcaaag acaaggtttg caacatacat tgttaatgct gattgttccc
    25801 ttgcctttat gtctgcttgc ttattttgct tatatctatt ttaagcccct acctcaggta
    25861 attccgtatt gggtgttttt agttcaagcg ggaactgtaa ttttgggaac tatgattaac
    25921 tattttattt tgcatagttt tgaacagcaa gttgccctac aggaaaatcc tattgaagtg
    25981 attactcctc cggaaatgga aagtaatgga aacaaagaac aggaaaatga cacaccattt
    26041 gcctaatatt ttaaccgtct tcaggataat tctggtgccg gtatttctct atttcctgtt
    26101 tttagctaag ctgccggcaa atattttgat agcactgatt attttcattg ttgcctgttt
    26161 tacggattat ctggatggaa tgttagcccg taaaatgaac ataataagtg attttggcaa
    26221 gctgatggac cctttagcag ataaactgat tgttctttct gctttggcag gtttatgttg
    26281 gcttcctcct tttcaggtta gtattgtggt ctttttcata atctttctgc gtgaacttgt
    26341 aattacaatt ctgcgggaaa tttatcagaa aaaaggtatg gtggtgcctg cggatttttt
    26401 cggcaagctg aaaaccgttt tgcaaatggc aggaatcatt gttgcctttg ctttttgggt
    26461 atttattaat cccgttcctc cgctggtaat tctgataatc aatgtctggt tttggtttgt
    26521 ggcattgatt actgtgctca gcggtttaaa ctatattatt acgatagcaa aaaggagtaa
    26581 ctatgcgtaa atatatgatt tttctcctgc ttttactggt tctattcgcc tgttccaaag
    26641 aaggaggcaa aatagaccgt tacactattt acgataaagt aattgaccat tccaaaccct
    26701 tagctatggg agatgacagg gatgtgtatg tattttgtga ttctcttgat tggcaaggtc
    26761 ttaaaccttt tattggcagt gctatagaac aacagataat tatagtttat ccggagcagt
    26821 attttaacct ggtgatggct gatatcaagg atgtgaaaag attatctaaa tataaaaatc
    26881 tcgtttttat cggtcacctg gaaagtgatt tacccgtttc tgcctatatg aaaagagtgc
    26941 tcagtcagga ttttattaaa agagtgcaac aaacaggtgg cgaacttttt accagtgaaa
    27001 acctcaattg ctgtgatcag attgtgcttt tcttgttggg taaggatgct aaaaccctgc
    27061 aaaaaattgg tgccctacaa gcagaaaata tcttttctct gctcttaaaa cgctttacga
    27121 atcgccaggg ttattatgct tatcagggaa aagtgataga cccctccttt tttgataatt
    27181 atcctttttc cctgaaaatt cctgatacct atcgtttgta ttccaatgat aaagttggca
    27241 ggtttctttg ttttctttac agagcccgac tggaaaatag agaaattccc gataaataca
    27301 tttctgttta ctatgaaccg atgccggaaa acaaagtgga cttaaattgg ctgattgcaa
    27361 agcgccagga aataggaaaa aaatactttg aaggagatga atttgatcct gaacttgtgc
    27421 ggaaagaacc ctttactttt aaaaaacatc ctggctatcg tctcttgggt gcctggaaaa
    27481 atatgaaata tgcaatcgga ggcggttttc aaagttatgg cttttgggat gaaaaaacca
    27541 aaactgctta cattgtagat agcagcgttt attttcctgc tggagacaaa cttcccattc
    27601 tggtggaatt gtatgttatc tccaattccc tggaaattaa atgaaaacta tcttgtcttg
    27661 caagcagtta ctactgtggc tggctttttt ccttttgctt ttatctgctt gtgcaggacc
    27721 tcaaacaaca gctcaaagaa ctattataga taattctcaa cccaatattc cttctcttta
    27781 ttattatatt tctgcttcgt taaaacatta tgaaggggac tttctaacag cagataatta
    27841 ttatcatctg gctttggaac aggactttcg tagctatcag attaaaaaac agattttaat
    27901 taatagtgct tatgcctttt taagtaaaca acaggatgca gaaataacgg ttaggcaatt
    27961 tgacaaagcc agaaaagaaa tgatttttga taacgatctt ttaaatgccg cttattctgt
    28021 ttatagcaat gccaataatg aagaaggact tgcctgggta gtttcggaaa gtgtgttata
    28081 ttatccttcc actttaagtt ttctgcgtaa attctatttt gattacagca aaaacaaaaa
    28141 gagcgatgaa aaccttctgg aacttgctct taaatatgca gataacaatc ctgaagatat
    28201 aattcttacc gctaaaatgt ataccctggt aaatcctaaa cgctccatag ctcttttaca
    28261 agaggcatac gaactggagc ccaaaaaaga aacggataat ctgcttagtg aaattacact
    28321 ccaatatctg ggaaaagaag aagctctgaa aaaattcagg acttacagtt atcctgaaga
    28381 taaggacagg atgttatact ttttgcagat ggcaaatcgt aatcgtcgcc tggaagtagt
    28441 taatacttta agccctatat tgctgaatac aggtgattct tctttgatag gagaactggc
    28501 tttttctgca tatctggaag ataaaacaga tatcttgcag gaaatttccc gctttttatt
    28561 aaccaaagat gcagaccctc ttaccgattc taaagtggtt tcctttcttt ttgccgaagc
    28621 tcttttttcc gaaaagttgc cggaaccttc cctttatctt gattacttta attcggcaca
    28681 agatgtccaa gatgtgattt tgtatgcaat gctgaaacgc tctatgcagg caaaatccct
    28741 tgaaaaagaa acaaatatcc tgttttcgga agaatttgtt tctgcagttg agaagcgtct
    28801 gccggaaaat gttttttccc gttatctgaa aattgcttca caaattaagt ccgttgaaga
    28861 tgaggacttt cttcaagcca gggctgaact ttgcaattac ttcctccaaa aagagttagg
    28921 ggaactggag gattggactt tcctgcttca gtattaccaa aatgagggca gggaagaaga
    28981 aaaaattgct actctgcgta aagctgttcg taagtttccg gataatccct tatttcttaa
    29041 tgacttgggt tattcccttt tggactatcc tcagtttatt gaagagggcg gcttactgat
    29101 tcaaagagct gtcgctttag accctacaaa tccttattat caagatagtt tagcttggtt
    29161 ctattatcta actgcaaatt tttctaaggc aatggaacat atcacaattc ttatgcaaat
    29221 gcaagatgct ccgggagaaa tatcctatca tattggaatg attcttttgg ctaatcaaca
    29281 aaaagaagag gcaattaaat acttccaatc cgccttggaa gatgatcaaa cccccatcta
    29341 tcaggaaaaa gcgaaaaaag cactaaacga actaaaaact aactaaacaa tagaacggtg
    29401 ggcaaagaaa aatatctgat ttatatccat tgaaaagttt tgtagaaatg atagataaag
    29461 ttgaaggatc tgaacccggt gcaaggggag aaaaaatact caaatatact tctatgaaga
    29521 aaaaagttcc gtaggaacga caggttctaa caacgggttt ttaacccggt gataagaaaa
    29581 gaaccaacaa aactatatat attggggaaa aaagttccgc aggaacgaca gatattaaca
    29641 acgggtttta acccggtgat aagaaagcag aaaagtcatt atctcctttc ccgagaaaaa
    29701 gttccgtagg aacgacagat attaacaacg ggttttaacc cggtgataag aaaagaacca
    29761 acaaaactat atttattggg gaaaaaagtt ccgtaggaac gacagaatta tgattttctc
    29821 tgataaatat tcattttttc tggttaatga cttccttctc tacataagtc ctatctattt
    29881 tattgtatat ttacctgctg gatcttttgc tttttcttct tgttgctttt tgcgggttga
    29941 aacccgctat tagcatctgt cgttcctacg gaacttttta ttatagtggt aaggtttttc
    30001 tcattttcac cgggttcaaa acccgttgtt agtatctgcc atccctaacg ggattttttt
    30061 gattttaaca acgggtttca acccgtggaa aagaaagcag aaaaatcatt atctcctttc
    30121 ccgagaaaga gttccgtagg aacgacaggt tctaacaacg ggttttagcc cggtgaaaag
    30181 aaagcaggag agtcattacc tcttttttcc taaaaaaagt tccgtaggaa cgacagataa
    30241 tagtgtcgga ttttgaaccc ggttcaacaa ttcagagtat tgtaaactct ttggcaaaga
    30301 aagaaattgt caatcctttt cccaactcca atattatgtt atggtgtcag ttacaagaaa
    30361 ttagcaggca aggcagggga ctgctaattt tttacttgac atataggtaa tgctgattat
    30421 attttc
//
LOCUS       DAOOZV010000017        30222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1662_length_30222_cov_53.923758, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000017 DAOOZV010000000
VERSION     DAOOZV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_30222_cov_53.923758"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2109)
                     /locus_tag="PLW08_03110"
     CDS             complement(70..2109)
                     /locus_tag="PLW08_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPL51624.1"
                     /translation="MSRHLHFYLGIVLMLVSIFLTAENVPVTLSKNNSNNDSDILAEY
                     NGGVITRKDIELKISKLPPQYQSRYKTVGGQMEVLNAIAAEEVFYQKARQMGLENDPI
                     VLEHISAIEKRFYIQEYYKRNVSDLVFITDDDLLSYYNQNLSQFYQYPYVTIDYIQAE
                     NEDAAKKALAELKKGISFATVSDTYNQNSYAKGLKGRIKNVRLNGNIPGIGNDIELEN
                     IIRNAQVDTLNFIGPVKTDYGWHILRIVDWIEGYQKDFNEVKPEIEQRVRPMKERELL
                     NALMDNLKIKYAVMVDSAIVNTISLDPNKRNSNKPILDNIIVSSSEPSLQYTVRQLLQ
                     NFDKLSPQEQLFINKGGGINLLIEQELTQGLMYLEAKANGYEKYFSENEDYLQAKRSY
                     VLRRAFEKLVSDTIEITDEEIAERYEREKERFSTPAYRTIEVLFFDNKKDANTAWKKY
                     SKAYKKHNEQKMQEIIKKYSLKPENAILDHQYNNGIITGIGQDAEFSSQIWNNPVGYL
                     SPVFTSARGDIVFFRTLKETPSSYKSKEETETQLRRMIKSEKEKAKMEEVTEDLFVEF
                     NMKKYPERITLTMTAEELFNYADNAARQRNFKDAIMYYDQIINNYKNNSDDYKASFMK
                     AFLIAEEMKQKDLALQLFKEFLQKYPTGDLNDSAQFMIDSLEGRIPEDFEPLRED"
     gene            2298..2735
                     /locus_tag="PLW08_03115"
     CDS             2298..2735
                     /locus_tag="PLW08_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL51625.1"
                     /translation="MLRKLKNQKGFTLIEILVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAINTIRKTYDIYMQTNGTTEGFSMEQALKEASLGESTLKNWKFEVVGNPPKKFI
                     ATSTQDFAGGEGKQVWYDVTDAKFHGYGIDTWTNPETGGMETD"
     gene            2755..3810
                     /locus_tag="PLW08_03120"
     CDS             2755..3810
                     /locus_tag="PLW08_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPL51626.1"
                     /translation="MTIHELLRFTAEAGASDLHIAAGSHPMVRVNGRMKKLNLPIQSP
                     EEVETLVYGVMNESQQALFKKNLEIDFSTKLSNDVRFRVNAFHQINGVSCAFRVIPNE
                     IKSYEELHLPDILKRLTTKEKGLILVTGPTGSGKSTTLATMIDTINDSRNCHIITIED
                     PIEFVHRSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQVRSMLSESLEAVIAQTLLPTKDGKG
                     RVPAVEIMIANAAVRNLIREEKTYQIPSIIQASTKEGMQTRDQSLYNLVMNNLIERTI
                     AEEFADNPKQFATGAGF"
     gene            3906..4289
                     /locus_tag="PLW08_03125"
     CDS             3906..4289
                     /locus_tag="PLW08_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51627.1"
                     /translation="MAEVLVVAIVMAVMIITIYIGIMYADKQVTKNYRHRVATLMLTG
                     ELEKQYTLYMKEGIFRPFTNMPVIIEQTDEVTVNGTISISVGKDRENYMSGNYDFTYL
                     IGEIRWIDPATEKEHYVRLREDFYE"
     gene            4300..4947
                     /locus_tag="PLW08_03130"
     CDS             4300..4947
                     /locus_tag="PLW08_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPL51628.1"
                     /translation="MKRMIFNEKGFTLTETVAVIAISSLLILTAAVGIGVFFRKYQEL
                     SAYVDLQKDMVSFLNVLKYGQHIGSRSSDIEFMGITSARELKLIRMTTIPGVSKGIEI
                     KPPISDLYPNDYVRYYLHDGFIKMEYRHRGTDSSSPTILFPEKGLKDRVIIEDFLISD
                     ANANNAIFKYRPNEKLCVINVDFKAKVKIRQNEFKSVHYKTVMAMKNMERPSPTN"
     gene            4967..6925
                     /locus_tag="PLW08_03135"
     CDS             4967..6925
                     /locus_tag="PLW08_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51629.1"
                     /translation="MINILKSESGNIAIAMLLVVIGAMSGLTMSSIAFRDTMATMAEL
                     EDIQCIHFLRTEASRGEAYLKVAAKRDPEIVGGVRTPERKIGMRGSDFQKTYKMQSLI
                     NKIYKELESEVVEVGGEIQASQTGASTTYYKIRSLVEGKTGTSDVYYGRKNLPLTRKY
                     GELSLVQDTGPVFMYFTDNEMSPNDTNVYFYGYDVINGPVHSNTDIQIKQLGGGNNNS
                     WPTFLALVTTAGEIISTPSSYPEEAIFRGGLIEHYDEYEFPENMIEIRQNGQRVGPSY
                     SEDRIMVIDIDDYNVSGWQGNITYRRQQIPLYLNNYPNGYPYPPGSETAINRFPTADT
                     TWVPLAPFSAHNGRAYYVESKLWIKGTIRGFQTWASADTMWLIGDLLYAGTPRGSDPL
                     SNTRDMLGLVSEKSIIIKYAYRNPADSLRIHTNMGSDSSNPVGGIWIYAAMAALGKGN
                     GNSFEDGVFTFEYQHPHGSIPAVKFNPSTDDPDVGPIVFDMIDLHRHYWPQSTAHPWP
                     ADLDFPWYNPIWPEANPYMERGTISIWGGVNQRRRGFVHRSMNDTEYPSNSGVWKPSI
                     DMCGGPCSTTATVVQLFQNPTVNVTLQCRHYPGAGGGQIGYKKNYNYDSRMYRVKPPF
                     WPYFKKQGERLPLEQGSWYLKKPPKSLI"
     gene            6967..8037
                     /gene="pilM"
                     /locus_tag="PLW08_03140"
     CDS             6967..8037
                     /gene="pilM"
                     /locus_tag="PLW08_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPL51630.1"
                     /translation="MKKKKQPRFKETVGIDIGTHSIKIVHLKKLHEGFKLLNYEISPT
                     VATGTEYVPSDLLPERYGPVLCEMLNTLKINPKRIQHLVTSIGGDNTSIKQIKTIFLP
                     DEELESALFFEAKKHIPISGTDMILDYQVISLEEKTNNMNILLAATSKDVLNEHTNVL
                     MTAGLAPNIVDIDSLAVVNSFILNTFVEDGVYILLNLGAHRTNMIIWGPGAKLFARDI
                     PYGGYNFTRDIMRKRQLEWAEAEHHKLEFGLGDNPAVENVQTVSMLDITEKPTEDSIV
                     DEVRRSLRFYVKEAGNSDFRKIYLMGGTAKLKGLKEFIEEKVAIPTEIFMPFINVEMP
                     EKFQDKKDPQLALAIGLAMRLE"
     gene            8053..8646
                     /locus_tag="PLW08_03145"
     CDS             8053..8646
                     /locus_tag="PLW08_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51631.1"
                     /translation="METTFYFKINLNKYGEMRLQAEREKKQFINAVIIFAVGLVILIA
                     AWIYINGMINTRVENRQRYLNELQKELASYETSEDYLSSKDLDKLAETFNNRIFWAKK
                     MVALSNEINEKLAVRKFTYNNGVLTLNGITEVDKNVKELDLIQSFIDRLKANEEISND
                     FPRIKSGFITRQIIKDTAILEFVIECYSKEASGGGIR"
     gene            8643..9194
                     /gene="pilO"
                     /locus_tag="PLW08_03150"
     CDS             8643..9194
                     /gene="pilO"
                     /locus_tag="PLW08_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPL51632.1"
                     /translation="MRERYLIFILCILLITILFFMFTINSVTAKQHKIKELDRKIKTA
                     QEKLNSARIMDQQLSQFSRIIDNSLTREASFSFDEINAFKTTIGRLADQRTITINKLS
                     DSNKFSLPGLVETTYNLELEATYVQIGQFISDLESMDNIIKIQALDISPLAVSDKEVT
                     APNAPNRYRVTLELSVFKVKREA"
     gene            9196..9735
                     /locus_tag="PLW08_03155"
     CDS             9196..9735
                     /locus_tag="PLW08_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51633.1"
                     /translation="MQQKFIKDLAIALIVLSLFAMAVRLVSLYNTWSKIPEKSVHTKE
                     SVSDTLLTKIKSIENSIQDRKMFVFNSSKDPLRQGNIIKDKVDREKEYQEMLKNTFRL
                     AATARDEYGNKIAYIEYQGELLEAHIGDTVKGRHILDIQDNSIRYSYNGTITTTELAP
                     RPARPIEETTYGSGTSGNW"
     gene            9763..11163
                     /locus_tag="PLW08_03160"
     CDS             9763..11163
                     /locus_tag="PLW08_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPL51634.1"
                     /translation="MKHIRKFKSHITLVLVLSFLACITLSAQQRRDPILDTKVTFNAD
                     DATLSSVFKALSRLSNTNIVLAIDQAGTAEKEEKRVTINIKDVPIETAVSLVASSAGL
                     SYRVIGSNTFLVGQKQNIMEEIGERSDIIFLNNLDAKKVSDALQNTSGKITPLEGQNA
                     LMVYGNPETFKEIEKLVKSIDIQQQQIEIRVRLIEVHLNEAKQIGVDWSKLNHLTTIL
                     AEDPVNQYGTGLPYNYADATGYLPHGDPTDFGVIPDEQYFQRINGFNDIGHFSRQLTA
                     FDVTIDWLLENNAAQLLTDTRVTALNGELATMHIGEVVPFVVTDKENQLQVERSETGI
                     ILNVTPKVNKDGNITMNINPEVSSVTELVGGYIPRTKVRRVSSAVTVPNEHKIIVGGL
                     LNSNITQKINKIPLLGDLPLIGKIFQHRYELVENTDLIIEITPRLVSMQETAPTPKLD
                     KRLTRTLIQYEEEEEE"
     gene            11165..13420
                     /locus_tag="PLW08_03165"
     CDS             11165..13420
                     /locus_tag="PLW08_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51635.1"
                     /translation="MKKYSSLMSILTLLLIILVSFSASCDKRNPPPILAPVPLPPSAS
                     QERYITRMTANPDRIYADYNITYSIVSAEIKDGEGFGVPNQMVKFKTNLGRIISDVPT
                     DSSGVAKTTFYDDGDIGIATISAIVRNYDASGDTLIWQDSTAITVEIKEIPPVENVEL
                     KLKDLAPPYTMSIMQVITVTAITTNAEGQEVPNNTLVTFSCNERGYFVDSGGSEIGTT
                     TIAGTYNGRATVKYNSGTRATTMPGEEPAMITATVGGVSDTKEIVIEPGNPANIRLKS
                     YVQVDNELIEADTSSVNSPNWIFVKAQLTDIYHNACPYQPVKFSTDIGSFLNTTQTVT
                     QNTQLDGIASVRFTPGLSAGAASIQATANNDTLKAVTIFMINSSELYSLDFTQSSQIN
                     LNVANTGGVESAILRVKLRDINGNLIDAPQKVYFRIANINAPAGANINGYPQQDSVEV
                     ISNGGEAQVSVNSGTESGIVKIKVSWVSENGNRYIYALKTNIVIHSGPPVPGGIISFV
                     SGFDSGQELGGGLWRVQCGAIVKDIYNNPVDWGTTVWFSIVDNANCEIEASAHVGNQN
                     AEGDSTAGVAYTYVTYDGTLTYHTITIRANCGDDPYGVPIFGDTPCVLPLNGPEAYIE
                     AQPLVLMFDLNQTNYEFSDIYFYLTDAQGCQISNAKVMFLALDGGHFIYSSNAIYDPA
                     SLPNEYWRLLTNQNGYAIARIAFSPVEAPPPVEEIPQPVPMRVRATLVEALLNRETVV
                     TLIKFPGTAPW"
     gene            13535..15244
                     /locus_tag="PLW08_03170"
     CDS             13535..15244
                     /locus_tag="PLW08_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPL51636.1"
                     /translation="MVYNPQFARLGDILVHEGYVTEEQVKDALLKQGNFGLKLGETLI
                     KLGYLTENELLEALHKQLGYDVVQDKELMDLDINVVSSIPEPYAKENKVLALREEGDG
                     VVVAMTDPENLIVSDSLEKILGKNIKPVLIGNSSLQDAIEKYYKSIRTTTEVEDAVGG
                     FEFVAVDEDENEITIAAATEDVNAPVVKMINLIINEAIKAGATDIHIEPLTKISRIRY
                     RVDGALREVMTPPIGMHASLISLVKVMSKLNIAERRLPQDGHISLKTSMKSVDVRVSI
                     TPTVLGEKVVMRLLDKGEFGFKLTTLGFEEDDMEIFKKIIRRPYGIIIVSGPTGSGKS
                     TTLHAALKEIENIESNIITVEDPVEYRLEGVTQIETKEQIGLTFGSALRSVLRQDPDI
                     VLIGEIRDEETADIAIKFSLTGHLVFTTLHANDAPATITRMIDIGIKPYLVGSSLSLV
                     MAQRLVRKICKYCIKDYIPTEQEIQDAGLTPEEASKINFKIGAGCVHCDNTGYSGREG
                     IFELLTINPEIRRIIYEGGNQDLIREAALRNGMRTLHDAAIAKMKKGITTIKEVIKMT
                     VVE"
     gene            15269..16522
                     /locus_tag="PLW08_03175"
     CDS             15269..16522
                     /locus_tag="PLW08_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPL51637.1"
                     /translation="MPNFAYIIKDAKGARIEGMIKAETLDMAVDKLAKEGNTIISVKA
                     AAEGAFKGKLSLFDKVMLAIYKFRTGVSLKSLVFFTRQLSTMFSAGLTIEKAISDLEK
                     EEKSKKFAKVLRKLSDDIRKGYSLSEAMQQHPGVFNPLYIALVTAGEVSGTLHTVLDE
                     LADYLEKMEDTRRKVYSALAYPIFIVIFLFFVVWGMFYYIIPMFADVYANFHADLPAP
                     TTLAINISHFITNNVFMFIVIVLLVIFTFFLINLTDKGRYVFDSIKLKIPVIGKVISN
                     SIMSKFSRTFSILMAAGVPIMDTMELTENVVQNAVIESAVRKARVMVKEGYGVANAFR
                     RTGMFPPTLLQMIATGEETGDMDKLLSKAAEFYEKLVDSVIDRLTSLIEPLLIIFMAV
                     IVGSIIVVIYLPIFDLGEAMSQGLR"
     assembly_gap    16777..16876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17476..20055
                     /locus_tag="PLW08_03180"
     CDS             17476..20055
                     /locus_tag="PLW08_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPL51638.1"
                     /translation="MKKYLCILVSIFPLLSFGAPFWNIPSSVSQPDGSVLNLFASGDE
                     YANRLHDANGYTIIQSAVDGYYYYAILEKGEPVPSAYRYGTIDPSAYGLIPHINISRE
                     ARQRKIDFMNAQKRRNERGPNIGNVNNLCIYIRFSDQTEFEIPRSVYNARFNEVGDNA
                     VSLRSYFQKVSYNQLQFMTYHYPTCADIINLSYQDIHPRSYYLPYNAVTNPNGYIDDD
                     TRTYREHNLLMNAVYAVASEVPASLNIDADNDGHVDNVCFIIRGPHSAWSDLLWAHRW
                     VLFSYNVSIRNKRIQDYTFQPEDQNDVTTLCHEMFHSVGAPDLYHYEYDGLTPVGCWD
                     IMESGEGHMCMFMKYFYGQWISSLPLAQIGNTYTLNPVTSPTNNVYMYPIPGSNYEFL
                     VFEYRKKGSDVFEEKLPGSGLLIYRIDQRFEGNADGPPEGIYVYRPNGTLTHNGLVAE
                     VPFSADNYRTAFNVYTNPQSFLNNGNTFPINLKNITSTGETISFTLASPTESMAPVIS
                     SISPTSGSILPAETIQLNPQITDPANALLRVEYTLDGVLVYTANSEPFSGEINGNLLT
                     AGVHNIGITAISSSGLTTNLNVYYRIIDPNLQNWFSWISPEPLWTEYSRGAIPIKVAV
                     NMDLGTQEYLVKGLRFKITPDPWGEPDIPGLVVAQINRFANGAITEQVLLSIGYIFNP
                     GYDPNFIYTISDTTSISGQIAVILDLFEYQNICFDQNASCGNSWITEPNRIWTDALGR
                     GVVGSAGIELLLQKPNVASDDPFIPAVNLSLSSYPNPFTETNKIKYSLPESGKVNLSI
                     YNIKGQKVTTLQAENKKSGNYELEWNGKDSSGNKVSSGLYFCRLETNGKIVTTKLLKL
                     KGM"
     gene            20583..21926
                     /gene="gcvPA"
                     /locus_tag="PLW08_03185"
     CDS             20583..21926
                     /gene="gcvPA"
                     /locus_tag="PLW08_03185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPL51639.1"
                     /translation="MPYISNTERDRQKILKTIGVNKFEDLLTAIPEKLRMKKPLALSE
                     PLSELEINKNIQAKKSKNISCEKVNSFLGGGIYDHFIPSAIETIVSRPEFLTAYTPYQ
                     AEVSQGTLQYIFEYQTLICELTGMEIANAGMYDGASSIAEAILMAVRKNHLTKAILPA
                     TLNPNYVQVIKAYTEGNGIELLFAPEKDGVTDLSALESMLNDTIGSVVIQTPNFYGNI
                     EDAKAIESIVHKNPKCLLIACVDPVSLAILMPPAEYNADIAVGEGQALGNNMFFGGPL
                     FGFFATKLDMARLMPGRIVGGTIDKDGKKAYVLTLQAREQHIRRAKATSNICSNESLC
                     TLAAVVYLCLLGKEGLVEIATQSAQKAHYTANELCKIPGFALAYPKTYFFKEFVLKTP
                     KKAEEIIKALLSGNIYAGIDLAPYGKPDELMIAVTEKKTKAEIDSFVQAMREVVK"
     gene            21923..23377
                     /gene="gcvPB"
                     /locus_tag="PLW08_03190"
     CDS             21923..23377
                     /gene="gcvPB"
                     /locus_tag="PLW08_03190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPL51640.1"
                     /translation="MSKTIFEHSTAGRKGVTLPKREIDIPVATMFPETMLRTKKARLP
                     EVSELDVMRHYITLSQKNHFIEKGLYPLGSCTMKYNPKLHETLIRDSAFTSLHPYQPE
                     ETLQGALELMYELQKDLAEIAGMAGVTLQPSAGAQGEFTGIKIVSAYHKAKGNTHKDT
                     IIIPDSAHGTNPATCSLVGYKTVELASNEKGRCDIAKLRELVNENTAGIMITNPSTLG
                     LFETQIEEIADIIHSVDALMYMDGANLNALLGIVQPGKIGFDLMHFNLHKTFATPHGG
                     GGPGSGPVAVNENLLPFLPVPVITKDEKGYHLSFANEKTSIGKVHSFYGNFAVLIRAY
                     IYIKMLGAEGLQRVSENAILNANYLMKRLNPYYHIEHQEPCMHEFVMDGTWQKKESGI
                     TTLDIAKRLLDKGFHAPTIYFPLIIPEAMMVEPTETESLESLDAFATAMIEIAAECKE
                     NPELVKEAPLTTPVRRVDDARAVKMLEPIFPLQD"
     gene            23408..24037
                     /locus_tag="PLW08_03195"
     CDS             23408..24037
                     /locus_tag="PLW08_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPL51641.1"
                     /translation="MKKYILTLSVLLALFAFAYAQPVKLGFVNTDRLLLDSNEAAEVA
                     RLFALDKQNWTNQIKSLDEEIKQMERDFEIRKLTMNDAAKREAQSRIDTKKAEAGRLL
                     EEYFGDGGKAEQRYRELIDPLTLKIHNLIKKIAEDEKYTMIFDVSMGSVLYALPALDL
                     TDQILLELNKDTIKPTEGTTPEPPKPEGQKPPEIKPPEGFGDEFKPDKL"
     gene            24034..25083
                     /gene="lpxD"
                     /locus_tag="PLW08_03200"
     CDS             24034..25083
                     /gene="lpxD"
                     /locus_tag="PLW08_03200"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPL51642.1"
                     /translation="MKRFNIPINSELLTQICQGELKGKIPSVLCKVSEPEEADEQTII
                     FLEQEKFLEKVKASSAGLIITTEKFISELPERALLLVQKPYYSLMRLISWWLEQENKD
                     FIYTIQSTAIVAEDVRFEGEVAIGDNVVIGSGCILGKGVIISEGCSLGKNVSVGAGTK
                     LYPNVCVYDDCVIGRNCILHSGVIIGADGFGFMLIEGIQQKIPQVGNVVIGDGVEIGA
                     NSCIDRATLGSTIIGNGTKIDNLVQVGHNCIIGEHSILCAQVGLAGSTVVGDYVYLAG
                     QVGIADHLQIGNRAMVGAQSGVSTNIPDDGRYFGYPALEANLTKRIMAVQKNLPEMYH
                     FYLKAKKQETENGEK"
     gene            25083..26477
                     /locus_tag="PLW08_03205"
     CDS             25083..26477
                     /locus_tag="PLW08_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPL51643.1"
                     /translation="MEYKQTIGGESTYTGIGLHSGEISTIRFKPAKADEGIVFIRTDL
                     PGKPKIPADIDHVVDISRGTTIGINGATVATIEHVLSAITGMRIDNICIEINGPESPV
                     ADGSAVVFLNLLRQCGIVQQDSEREYFELDSPISFSAPEDNVDIVIVPSNELKITFMI
                     DYKHPYLGTQYTWLPSLDNYEKDFAGARTFCFINEILELKNMGLIKGGSLENALVIAE
                     PNISEAELKHLQDIFGYHKPITVSQEGILNSHPLRYYNEFVRHKVVDLIGDIALLGVP
                     IKGHILAARSGHKTNVELVKKLRQIQKKHQLQKIYQKDKSQNVVFDINAILRILPHRY
                     PFLLVDKIIEFIPGESLVGIKNVTINEPFFQGHFPGHPIMPGVLIIEGMAQAGGIMLL
                     NQMDNPQNYVAYFASIDNVKFRKPVLPGDTLRYELKVISLKRSLAKMHGETFVEGEKV
                     AEGDFLAMIMEREK"
     gene            26474..27247
                     /gene="lpxA"
                     /locus_tag="PLW08_03210"
     CDS             26474..27247
                     /gene="lpxA"
                     /locus_tag="PLW08_03210"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPL51644.1"
                     /translation="MTIIHPTAIIEEGAVIGDNCYIGPYCHIGKDVVLGSNNNLIANV
                     TILGDTIIGDGNKIFPYAVLGTEPQDLKYKGEPTRLRVGSNNTIREFVTINCSNQMEE
                     DTVVGDNNLLMEYAHIAHNSQIGSGCVIANVVQCGGHIHIGDFATVGGLTAIHQFVHI
                     GAYAFVGGASATNKDIVPFSRGQGNPYKTVGLNSIGLMRKGFASETIAAIKEIYNLFY
                     RSGLNTSQALEKALQIPNPTPEQIIFIQFVQNAQRGISK"
     gene            complement(27835..29148)
                     /locus_tag="PLW08_03215"
     CDS             complement(27835..29148)
                     /locus_tag="PLW08_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPL51645.1"
                     /translation="MANKDQSTLFDREELSTKTVPLAEKLRPQTLEELLGQTKLIAEN
                     SPLRQMIESGEYHSFILWGPPGSGKTTIARIIEKNSGYNFIRFSAVLASISDVKAVMK
                     EADYLHRTQNKRSILFIDEIHRFNKSQQDAFLPYVESGAIILIGATTENPSFEVIPAL
                     LSRCTVFVLEPLSENDLKIILKRGFAELKLEEDEEVINWMAQNAGGDARRVLNDLELV
                     LPYLQKKPHPKTEELAKSLAKKTMFYDKNREEHYNLISALHKSLRGSDPQAGLYWLAR
                     MLEAGEDPRYIVRRLIRFASEDVGLADPNALVQAIAVKEAVLFIGMPEASTALAQLVV
                     YLATAPKSNALYTAYEEAAEDARKTSHYGVPLHIRNAPTQLMKDLNYGKDYHYDHEYE
                     YKYYYQKYFPEQMPEKIYYKPGTYGFEKEIQKRIDWWAKLKKEQK"
     gene            complement(29160..29627)
                     /locus_tag="PLW08_03220"
     CDS             complement(29160..29627)
                     /locus_tag="PLW08_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL51646.1"
                     /translation="MTLEDSYRMVIAEEIRAQKMYQALAKSFAKPETSNVFKELVVLE
                     KMHEEKVRSAFAKEFPGQTITLQEEPIKEMQGVNWNDPQEVLEFAITKEEESVELYKN
                     MAEQTREPEIKKQLLQFAKEEEGHKSILLSEIQRLQGALQWFDPSELSGLMED"
BASE COUNT         9444 a         5465 c         6147 g         9066 t
ORIGIN      
        1 ttttccgaaa tgatataagt tacgatgaac tacaagatgt tgttcttccg caaagaaaca
       61 gcaactctcc taatcttctc tgagaggttc aaaatcctcc ggaattcttc cttccaagct
      121 gtcaatcata aattgggcgg aatcattaag gtcaccggta gggtattttt ggagaaattc
      181 cttaaaaagt tgcagagcca ggtctttttg tttcatttct tcggcaatta ggaatgcctt
      241 cataaaggaa gctttatagt catcggagtt atttttgtaa ttattaataa tttggtcata
      301 atacataata gcatctttga aattgcgttg tctggcagca ttatcagcat agttgaaaag
      361 ttcttctgca gtcatagtaa gagtaatgcg ttcaggatat tttttcatat tgaattccac
      421 gaagaggtct tccgtaactt cttccatttt agctttttct ttttcggatt ttatcattct
      481 tcgtaattgg gtttctgttt cttctttgct tttataggaa ctgggtgttt ctttcagagt
      541 gcggaagaaa acaatatctc ctctggcgga agtaaaaaca gggcttaaat agccaaccgg
      601 attattccaa atttgactgg agaattcggc atcctgtcca attccggtaa taattccatt
      661 attgtattga tgatccaaaa tggcattttc cggtttaaga gaatattttt taataatttc
      721 ctgcattttt tgttcattat gtttcttgta tgctttgctg tattttttcc aggcagtgtt
      781 ggcatctttt ttattatcaa agaagagcac ttcaatagtt ctatatgcag gagtggaaaa
      841 tctttctttt tcgcgttcat agcgttcagc aatttcttcg tctgtaattt ctatggtgtc
      901 ggaaaccagt ttttcaaagg cacggcgcag gacataagat ctttttgctt gaagataatc
      961 ttcattttcg gaaaaatatt tttcatagcc attagcttta gcttccagat acataagacc
     1021 ctgtgtaagt tcttgctcta taagcagatt aattccccca cccttattta taaaaagctg
     1081 ttcctgagga gaaagtttat caaaattctg cagtaattga cgcacagtat attgtaaact
     1141 cggttcgctg gaacttacga tgatattatc cagaatgggt ttattactgt ttcgtttatt
     1201 aggatccaag ctaatggtat ttacgatagc gctgtctacc attactgcat actttatttt
     1261 cagattgtcc attaaagcat ttagcagctc ccgttctttc ataggacgaa cacgctgttc
     1321 aatttcgggc ttaacttcgt taaaatcctt ttggtaacct tcaatccagt ccactattct
     1381 taaaatatgc caaccgtaat cggtttttac gggaccaata aagttaaggg tatccacttg
     1441 agcattgcga ataatgtttt ccaattcaat atcgttgcca atacctggaa tattaccgtt
     1501 taaacggaca tttttaattc tgccttttaa acccttggca taactatttt ggttatatgt
     1561 atcggagaca gttgcaaaag aaataccttt tttcaattct gccaatgcct tttttgcagc
     1621 atcttcgttt tcggcttgga tataatcaat agtgacataa ggatattggt agaattgtga
     1681 tagattttga ttataatagg aaagcaaatc gtcatcagta ataaatacta aatcagatac
     1741 attgcgttta taatattcct gaatataaaa acgcttttct atggcagaaa tatgttccaa
     1801 aacgatagga tcattttcca aacccatctg cctggctttt tggtaaaaaa cctcttcggc
     1861 agcaatagca tttagcactt ccatctgccc tcccacagtt ttataccgtg attgatactg
     1921 aggaggcagt ttggaaattt tgagttcaat atccttacgg gtaataactc caccgttata
     1981 ttctgctaat atatccgaat cgttatttga gttattttta cttaatgtaa cgggtacatt
     2041 ttctgctgtc aaaaatatac tgacgagcat caatacaatt cccaggtaaa aatgtagatg
     2101 acgggacatt cttatactcc ttatcatatt gcttaaaata atagatgtca catttataaa
     2161 agggcagaag aagtcaagac atttatagaa acataaactg ataacaaaac atcggtttta
     2221 atttttgctg aggttttggc acgcattgtg cattatagaa ataataaaaa aaaacaatcc
     2281 cataggagga taaacaaatg ctcaggaaac tgaaaaatca aaagggtttt accctgattg
     2341 aaatcttagt ggtggttatt attgtagcca ttttggctgc attggcagtt ccccgctatc
     2401 tgcgttatgt agaaaaatcc cgtagtacag aagcacaaac tgcaataaac accattcgta
     2461 aaacctacga tatctatatg caaaccaacg gaaccacaga aggtttttct atggaacaag
     2521 cattaaaaga agccagtttg ggtgaaagta ctcttaaaaa ctggaaattt gaagttgtcg
     2581 gtaacccccc gaaaaaattt attgctactt ccactcagga ctttgctggt ggtgaaggaa
     2641 aacaggtttg gtacgatgtt accgatgcca aattccatgg ttacggaatt gatacttgga
     2701 ctaatcccga aaccggagga atggaaactg actaactctt aggaggacag taaattgaca
     2761 atccatgaat tactgcgctt tactgcagaa gcaggtgcgt ctgatcttca tatagcagct
     2821 ggctcacatc ctatggttcg ggtaaatggc agaatgaaaa aattaaatct gccaattcaa
     2881 agtccggaag aggtggaaac acttgtttac ggagtgatga acgaatcaca gcaagctctc
     2941 tttaaaaaga atctggaaat tgacttttcc acgaaattga gcaatgatgt ccgattcaga
     3001 gtgaacgctt ttcaccagat aaatggtgtt tcctgtgctt tcagagtgat tcctaatgag
     3061 ataaagagtt atgaggaact ccatctgccc gatatcttaa aacgcttaac gacaaaagaa
     3121 aaagggctga ttctggttac aggacctaca ggaagcggaa aatcaactac tttggctaca
     3181 atgattgata ctataaatga tagccgtaac tgccatatta ttactattga agaccctatt
     3241 gaatttgtcc accgcagtaa aaacagctta atcaatcagc gtgaactggg tcatgatacc
     3301 tggagcttta cagctgcttt aagaagtgcc ttgcgtgaag accccgatgt gattttagtt
     3361 ggtgaaatgc gtgatctgga gacggtatct ttagcattaa cagctgctga aacaggtcac
     3421 ttggtttttg ctactttgca taccggaagc tgcacaaaat ccatagaccg gataatagat
     3481 atgtttccta aggaacaaca acagcaagtg cgttcaatgc tttcggaatc tctggaagcg
     3541 gttatagcac aaactttgtt accaactaaa gatggtaaag gtcgtgttcc ggctgtagaa
     3601 ataatgattg ctaatgctgc tgtgcgaaat ctgatccgtg aagaaaaaac ttaccagata
     3661 ccgtctataa ttcaagcaag cactaaagaa ggaatgcaaa ccagagatca atcgttgtat
     3721 aacctggtaa tgaataatct gattgaaaga acaattgctg aagaatttgc ggataatcct
     3781 aaacaatttg ctacaggagc aggtttttag gtatgccgaa agatatttat atcctacctg
     3841 agaacaataa aacaaaaaaa gcaaacggag tgtttagttt gctgagttct cagggagggt
     3901 atagtttggc ggaagtcctg gttgtagcaa tagtgatggc tgtaatgatt ataacgatat
     3961 atattggaat aatgtatgcc gataagcaag taaccaaaaa ttatcgtcat agagttgcaa
     4021 ctctgatgct tacaggtgaa ctggaaaagc aatatactct ctatatgaaa gagggtatct
     4081 ttcgcccctt cacaaatatg cccgtaataa ttgaacagac ggatgaagtg actgtgaatg
     4141 gaactatctc catatcagtc ggtaaagata gggaaaatta tatgagcgga aattatgatt
     4201 ttacctactt gattggcgaa atcagatgga ttgatcctgc aacggaaaaa gaacactatg
     4261 tccgtttgcg agaagatttt tacgaatagg ggatggatga tgaagcgaat gatatttaac
     4321 gagaagggat ttacattaac agaaacggtt gcagttattg caatttcttc tttgctgatt
     4381 ttaacagccg ctgtaggaat tggcgttttc tttcgtaaat atcaggaact gagtgcttat
     4441 gtggatttac aaaaggatat ggtttctttt ttaaatgtct taaaatacgg tcaacatatt
     4501 ggttcgcgtt cctcggatat tgaatttatg ggaattacca gtgcccggga gcttaaactt
     4561 attagaatga caactattcc aggtgtcagt aaaggaatag aaattaagcc cccgatttca
     4621 gacctctatc ctaatgatta tgtgagatat tatttgcatg acggttttat aaaaatggaa
     4681 tacagacatc ggggaacaga ttcatcatcg cctactattc tttttcccga aaaaggtttg
     4741 aaagataggg tgattattga ggacttttta attagtgatg ccaatgctaa taacgcgatt
     4801 tttaaatata gaccgaatga aaagctctgt gtaataaatg ttgatttcaa agccaaggtt
     4861 aagattcgcc aaaatgaatt taaaagtgtg cattataaaa cagttatggc gatgaaaaat
     4921 atggaaagac catctcctac aaattaaatt tgggaggttt aatattatga taaacatcct
     4981 taaaagcgaa agcggaaata ttgccattgc aatgctcctg gttgtaattg gagcaatgag
     5041 tggcttaact atgtccagta ttgcatttcg tgatactatg gctacaatgg cagaactgga
     5101 agacattcaa tgtattcatt ttcttcggac agaagcaagt agaggtgaag catatctgaa
     5161 agtagctgct aagcgcgatc ctgaaattgt aggaggagtt agaacaccgg aaagaaaaat
     5221 tggaatgaga ggaagcgact tccaaaaaac atacaaaatg caatccctta ttaataaaat
     5281 atataaagag ttggagtcag aagttgtaga agttggaggc gaaatccagg cgagccaaac
     5341 aggtgccagt actacctatt ataaaattag gtctttggta gaaggaaaaa cgggaacttc
     5401 cgatgtatat tatggaagaa aaaatctgcc gctaacccgt aaatatggag aattatcttt
     5461 agttcaggat acgggacctg tctttatgta ttttactgat aatgaaatgt ctcccaatga
     5521 tacgaatgtc tatttttatg gctatgatgt aataaacggt cctgtccata gcaataccga
     5581 tattcagata aaacagttag gaggtggaaa taataatagc tggcctacct ttttagcttt
     5641 agtaactaca gcaggagaaa taatctccac ccccagttcc tatcccgaag aagctatctt
     5701 tcggggtggt ttaattgaac attatgatga atatgaattc ccggagaata tgattgaaat
     5761 ccgtcaaaat ggacaaagag taggcccatc atatagcgaa gacaggataa tggttataga
     5821 tatagatgat tacaatgtat ctggttggca gggtaacata acttacaggc gccaacagat
     5881 tcctttatat ctgaataatt atcccaatgg ttatccctat cctcccggtt cagaaactgc
     5941 cataaatcgt tttcctacag ctgatacaac ctgggtaccg ttagctccat ttagtgcaca
     6001 taacggaaga gcttactatg tggaaagtaa actttggata aaagggacaa tcaggggttt
     6061 tcaaacctgg gcttctgcgg atacgatgtg gttgattgga gatttattat atgcaggtac
     6121 tccccgaggt agcgatccat tgtccaacac cagggatatg ttaggtttag tatcggagaa
     6181 atccattatt ataaaatatg cctatcgcaa tcctgctgat tctcttcgta tccatactaa
     6241 tatgggttct gatagttcca atccggtagg cggaatctgg atttatgctg ctatggcagc
     6301 tcttggaaaa ggaaatggta atagctttga agacggggtc tttacctttg aatatcaaca
     6361 tccgcatggc tcaattccgg ctgtaaaatt caatccttca accgatgatc cggatgtagg
     6421 acctattgta tttgatatga ttgatctgca tcgtcattat tggccccagt caactgctca
     6481 tccctggccc gccgatttgg attttccctg gtacaatcct atttggcctg aagcaaatcc
     6541 ttatatggaa aggggaacga taagtatttg gggaggagtt aatcaacgcc gtcgtggttt
     6601 tgttcataga agtatgaatg ataccgaata tccttccaat agcggtgttt ggaaaccttc
     6661 tatagatatg tgcggtgggc cttgttccac tacggcaacc gttgttcagc tgttccaaaa
     6721 tcccactgtg aatgtaactt tgcagtgtcg tcactatcct ggagcaggag gagggcaaat
     6781 tggttataaa aagaactata actatgattc ccgaatgtat agagttaaac cacctttttg
     6841 gccctatttc aaaaagcagg gagaacggtt acccttggaa cagggaagct ggtatttgaa
     6901 gaaaccgcct aagagcttaa tatagaaaca aataaagtaa taaataatga aagataaaga
     6961 ggaagaatga agaaaaaaaa acaacctcga ttcaaagaaa ccgtgggaat tgatattgga
     7021 actcacagca ttaaaattgt tcacctgaag aaattgcatg aaggatttaa actgctgaac
     7081 tatgaaatta gccccacggt tgctaccgga actgaatatg ttcccagtga tttacttccg
     7141 gagcgttatg gacccgttct ctgtgaaatg ctgaacacac taaaaattaa tcctaaacga
     7201 attcagcatt tggttacttc catcggaggt gataacacga gtattaaaca aatcaaaacc
     7261 atctttctcc ctgatgagga actggaatcg gctctgtttt ttgaagctaa gaaacacatt
     7321 cctattagtg ggacagatat gattctggat tatcaggtca ttagtttgga agaaaaaacc
     7381 aacaatatga atatcctgct ggctgctact tccaaagatg tattgaatga acataccaat
     7441 gttttaatga ccgcgggttt agcacccaat attgtagata tagattctct ggctgttgta
     7501 aacagcttta ttctgaatac ttttgtggaa gatggagttt atattttgtt aaatcttggt
     7561 gcgcatagaa caaatatgat tatctgggga ccaggagcca aattgtttgc tcgtgatatt
     7621 ccttacggcg gttataattt tacgcgggat attatgcgta agagacagct ggaatgggct
     7681 gaagctgaac accataaact ggaatttggt ttgggggata atcctgccgt agaaaatgtt
     7741 caaacagtta gtatgcttga tattaccgag aaacctaccg aggattccat tgtggatgaa
     7801 gttcgtcgtt ccttgcgctt ctatgttaaa gaagcaggta acagtgattt ccgcaaaatc
     7861 tatttaatgg gtggaactgc taaacttaag ggtttaaaag aattcataga agaaaaagta
     7921 gcaattccga cggagatttt tatgcccttt atcaatgtgg aaatgccgga aaaattccag
     7981 gacaaaaaag acccccaact tgctttggca atagggctgg ctatgcggtt ggaataaggg
     8041 aggatttttc tgatggaaac tacattttat tttaaaatta acctgaataa atatggtgaa
     8101 atgcgtttgc aagccgaacg cgaaaaaaag caatttatta atgctgtaat aatctttgct
     8161 gttgggcttg ttattcttat tgcggcatgg atttacatca atggtatgat taacacaaga
     8221 gttgaaaacc gacagagata cctgaatgaa ttgcagaaag aattggcaag ctatgaaaca
     8281 agtgaggact atctttccag taaagacctt gataagcttg cggaaacatt taataatcgt
     8341 atcttctggg ctaaaaaaat ggttgcctta agtaatgaga taaatgaaaa gctggcggtg
     8401 agaaaattca cctacaataa tggtgtcctt actcttaacg gcattaccga agttgataaa
     8461 aatgtaaaag aattagattt aattcagagt tttattgatc ggttaaaagc caatgaagaa
     8521 atcagtaatg attttcccag aatcaaatcg ggctttataa cccgacagat tattaaagat
     8581 actgctattc tggaatttgt aattgaatgt tatagcaaag aagcatctgg gggaggaata
     8641 cgatgagaga aagatacctg atattcattc tgtgcatctt gctgattaca atactgttct
     8701 ttatgtttac cataaacagt gttactgcaa aacaacataa gattaaagaa ctggatagaa
     8761 aaataaaaac tgctcaggaa aaactaaaca gtgcacggat tatggaccag caattaagcc
     8821 agttttcccg tattatagat aatagtttaa ccagggaagc cagttttagc tttgatgaaa
     8881 ttaatgcttt taagaccacc attggccgtt tggctgacca gcgaactatt actattaata
     8941 aactttccga cagcaataaa ttctcacttc caggtttggt agaaacaaca tataatctgg
     9001 aactggaagc tacttatgtg caaatcgggc agtttatatc cgatttggaa tctatggata
     9061 atattatcaa aattcaggca ttggatatca gcccgcttgc agtttccgat aaagaggtta
     9121 ctgctcccaa tgctccaaat cgttataggg ttaccttaga actttctgta ttcaaggtaa
     9181 aacgggaggc ataagatgca acagaaattt ataaaagacc tggcaatagc cctaattgtt
     9241 ctctcgcttt ttgctatggc tgttcgtctt gtaagtttat acaatacttg gagcaaaatt
     9301 ccggaaaaat cggttcatac aaaagaatct gtttcggata ctcttttaac caaaattaag
     9361 tccatagaaa attctatcca agaccgtaaa atgtttgtat tcaattcgtc taaagacccc
     9421 ttgcgtcaag gaaacatcat caaggacaaa gtggatagag agaaagaata ccaggaaatg
     9481 ttgaaaaaca ctttccgttt agccgctacg gctcgtgatg aatatggcaa taaaatcgct
     9541 tatattgaat atcaaggtga gcttcttgaa gcacacatag gagataccgt gaaaggacgc
     9601 catattttag atatccaaga taatagtatc cgctatagtt ataatggaac aattactact
     9661 acagaactcg ctccccgacc tgccagacca attgaagaaa cgacttatgg tagtggcaca
     9721 agcgggaatt ggtaattaaa tataatgata ctgggagtaa atatgaaaca cattagaaaa
     9781 ttcaaaagtc atattacact tgtcctcgtg ctatcttttt tggcttgtat cacacttagt
     9841 gctcagcaaa gaagagaccc tatcctggat acaaaagtga cttttaacgc agatgatgca
     9901 actctttcct cggtgtttaa agctctttcc agattgagca acaccaatat tgttttggct
     9961 attgatcaag caggaaccgc agagaaagaa gaaaagaggg ttactatcaa tattaaagat
    10021 gtacctattg aaacagccgt ctctctggtt gcaagctctg ccggtttatc ttatcgggta
    10081 attggctcca acactttcct tgtgggccaa aaacagaata ttatggaaga aatcggtgaa
    10141 cgcagtgaca tcatttttct aaacaatctg gatgccaaga aagttagcga tgcccttcaa
    10201 aatacaagcg ggaagattac ccccttggaa ggacaaaatg ccttaatggt ttatggcaat
    10261 ccggagacct tcaaagaaat agaaaaattg gttaaatcaa ttgacataca acaacaacag
    10321 atagaaataa gagtccggtt aattgaagtt caccttaatg aagcaaaaca aattggagtt
    10381 gactggagta aactaaatca tttaactaca attctggcag aagatcctgt aaaccaatat
    10441 ggaaccggct tgccatataa ttatgctgac gccacaggtt atttaccaca tggtgatcct
    10501 actgattttg gagttattcc tgatgaacaa tattttcagc gtataaacgg ttttaatgat
    10561 attggacatt tcagccgtca gctaactgct tttgatgtta ctattgactg gttgctggaa
    10621 aataatgctg ctcaattgct tactgataca agggttacag ctttaaatgg agaacttgcc
    10681 actatgcata ttggagaagt agtacccttc gtcgttacag ataaagaaaa tcagctgcag
    10741 gtagaacgtt ctgaaacagg tatcattctc aatgttacac ctaaagtaaa taaagacggt
    10801 aatattacaa tgaacatcaa tccagaagta agttcagtta cagaactggt tggagggtat
    10861 ataccccgca ctaaagtccg cagagtttct tcggcagtaa ctgttcctaa cgagcataaa
    10921 ataattgttg gtggattgct aaattccaat attacgcaaa agattaacaa aattcctttg
    10981 ttgggcgatt tgcctttaat tggcaaaata ttccagcata gatatgaact ggtagaaaac
    11041 accgatttga tcatagaaat aactcctcgg cttgtttcta tgcaggaaac cgctccaaca
    11101 ccaaaattgg ataagcgttt aacccgaact ctaattcaat atgaagaaga agaggaggag
    11161 taaaatgaaa aagtatagct ccttaatgtc aattcttaca ctcctgctga tcatcctggt
    11221 cagtttcagt gcaagttgtg ataaacgaaa tcctccgcca attttagctc cggttccctt
    11281 acctccgtcc gcatcacagg aaaggtatat aaccagaatg acagctaatc cggatagaat
    11341 ttatgccgat tacaacatca cttactctat tgtttctgcg gaaataaaag atggtgaggg
    11401 ttttggagtt cctaatcaga tggtaaaatt taaaaccaat ctgggcagga ttatttctga
    11461 tgtccctacc gatagcagcg gtgtagctaa aactactttc tatgatgacg gagatatcgg
    11521 tatcgctact atttcagcca tagttcgcaa ttatgatgcc agtggcgata ctttaatctg
    11581 gcaggattcc actgcaataa ctgtggaaat caaagaaatt cctccggttg aaaatgttga
    11641 actgaaactt aaggatcttg ctccacccta tacaatgtct attatgcagg tgataactgt
    11701 aactgcaatt actacgaatg cagaagggca agaggttccc aataatacat tagtaacttt
    11761 cagttgtaac gaaaggggtt actttgtaga tagtggcggg agtgaaatag gcacaactac
    11821 gatagcagga acatataatg gacgagctac ggtaaaatac aattccggaa ctcgtgccac
    11881 aactatgccg ggggaagaac cagcaatgat tacagcaaca gttggaggtg tttccgatac
    11941 taaggaaata gtaattgaac caggaaatcc tgccaatata agattaaaat cttatgttca
    12001 agtggataat gaacttatag aagcagatac aagttctgtt aacagtccta actggatttt
    12061 tgttaaagcg caattaacag atatttacca taatgcttgt ccctatcaac cggttaaatt
    12121 ttccacggat ataggttctt ttttgaatac tacgcagaca gttacgcaaa atactcaact
    12181 tgatggaatt gcttccgtga ggtttacacc gggattatct gctggagctg cctccattca
    12241 ggcaactgcc aataatgata ctttaaaggc agttaccatt tttatgataa attccagtga
    12301 attgtactcg ttggatttta ctcaatccag tcagattaac ttgaatgtag caaatacggg
    12361 tggtgtggaa tcggcaatat tacgggtaaa attaagagat ataaatggga atctgattga
    12421 tgctcctcaa aaagtttact ttagaatagc caatataaat gcgcctgccg gtgccaatat
    12481 taatggttat cctcagcagg attcggtgga ggttatctcc aatggaggtg aagcccaggt
    12541 ttcagtaaat agcggaacag aatccggtat tgtaaaaatt aaggtctcct gggtaagtga
    12601 aaatggaaat cgttatattt atgccttgaa aacgaatatt gtaattcatt caggacctcc
    12661 ggttccaggt ggaattattt ccttcgttag cggatttgat tccggacagg aattaggagg
    12721 tggattatgg cgtgtccaat gtggtgctat agttaaagat atatataata accctgttga
    12781 ttggggaaca actgtatggt tctcaattgt ggacaatgca aattgtgaaa tagaagcgtc
    12841 ggcgcacgtt ggtaaccaga atgctgaagg agattctact gccggtgttg cctacactta
    12901 tgtaacctac gatggaactt taacatatca cacaataact attcgtgcta attgtggcga
    12961 tgatccttat ggtgttccta tttttggtga cacaccttgt gttttaccct taaatggtcc
    13021 tgaagcatat atagaagcac aaccgctagt attgatgttt gatttgaacc aaacaaacta
    13081 tgaattttcg gatatctact tttatcttac ggatgcacaa ggttgtcaga taagcaatgc
    13141 caaggtaatg ttcctggcgt tggatggcgg tcattttatt tacagctcta atgctatcta
    13201 tgatcctgct tctctcccta acgaatattg gcgattgtta accaaccaaa atggatatgc
    13261 cattgccaga attgctttta gtcctgtaga agctcctcca ccagtggaag aaattcccca
    13321 acctgttcca atgagggtaa gagcaacatt agtagaagct ttgctcaatc gtgaaactgt
    13381 tgtcactctg atcaaattcc cggggactgc accctggtaa agaatacata ttaattttcc
    13441 cgatttgcgg gttggaatat tcggaacttt aattctgaaa aaaacaaccc gcgaattgag
    13501 aataaactga ataattttgt tataaggaaa aataatggtt tataatccac aatttgcccg
    13561 tctgggagat atcctggtac acgagggata tgtcacagaa gaacaagtga aagatgcatt
    13621 gcttaagcag ggaaatttcg gcttgaaatt aggtgagaca cttatcaagc tgggttatct
    13681 aacagaaaat gaacttcttg aagcattaca taaacaactt ggctatgatg ttgttcaaga
    13741 taaagaatta atggacctgg atataaatgt tgtaagttca attccggagc cctatgccaa
    13801 agaaaataaa gtattagctt tacgcgaaga gggcgatgga gtagtagtag caatgacaga
    13861 tccagaaaac ctgattgttt cggacagctt ggaaaaaata ttgggcaaaa atatcaagcc
    13921 ggttttaatt ggcaattctt ctttacagga tgccatagaa aagtattata aaagtattcg
    13981 gacaacaact gaggttgaag atgccgtagg cggttttgaa tttgtagcag tggatgaaga
    14041 cgagaacgaa atcactattg ctgctgctac ggaagatgtt aatgcaccag tagttaagat
    14101 gatcaatctt atcatcaatg aggcaattaa agccggagct actgatattc atattgaacc
    14161 tttaacgaaa atatcacgga ttcgttatcg ggtagatggt gccttgcgcg aagttatgac
    14221 gcctcccata ggaatgcatg ccagtttgat ttccctggtt aaggttatga gtaaattgaa
    14281 tattgccgaa cgtcgtttgc ctcaagatgg tcatatttcc ctaaaaacct cgatgaaaag
    14341 tgtagatgtt cgtgtttcca tcactcctac tgttttaggt gaaaaagtag taatgcgtct
    14401 tttagataag ggagaatttg gattcaaact tactacattg ggatttgaag aagacgatat
    14461 ggaaatcttt aaaaaaatta tccgtcggcc ttatggcatt attattgttt caggacctac
    14521 aggaagcgga aaatccacta ctcttcacgc ggcactaaaa gaaatagaaa acatagaatc
    14581 taatattata actgtggaag accccgttga ataccgtctg gaaggagtta cgcaaattga
    14641 aaccaaagag cagataggtc tcaccttcgg ttctgctttg cgttcagttt tgcgtcaaga
    14701 ccccgatatt gttctcattg gagaaattcg tgatgaagaa acagctgata ttgctattaa
    14761 attttcatta accggtcacc tggtttttac tactttgcat gccaatgatg cacctgcaac
    14821 tattacccgt atgatagata ttggtattaa accctatctt gttggttctt ccttatcact
    14881 ggtgatggca caacggttag tgcgtaaaat ttgtaagtat tgtatcaaag attatatccc
    14941 tacggagcag gaaattcagg atgccggttt aacacctgaa gaagcaagta aaatcaattt
    15001 taagattgga gctggatgtg ttcattgtga taatacaggt tattcgggaa gagaaggtat
    15061 ttttgaattg ctgaccataa atcctgaaat ccgcaggatt atctatgaag gcggaaatca
    15121 agaccttatt cgggaagcag cattaagaaa tggtatgcgg acccttcacg atgctgctat
    15181 tgctaaaatg aaaaagggaa taaccacaat caaggaagtc atcaagatga ctgtagttga
    15241 ataataaatt tgaggaaggt aaatcgctat gcccaatttt gcctatataa ttaaggatgc
    15301 caagggagcc agaattgagg gtatgatcaa agctgaaacc cttgatatgg ctgtagacaa
    15361 acttgccaaa gaaggaaaca ctattatcag cgttaaagct gcagcggaag gtgcttttaa
    15421 gggtaaactc tctctctttg acaaagttat gctggctatt tataagttta gaaccggcgt
    15481 aagcctaaaa tccctcgtct tttttacgcg tcagctttct actatgtttt cagcaggtct
    15541 tactattgaa aaagctatat cagacctgga aaaggaagag aaaagcaaaa aatttgccaa
    15601 agtcctgcgt aagctctcgg atgatatccg taaaggatat tctctttctg aagcaatgca
    15661 acaacatccg ggagtgttta atccgctata tatagcttta gttactgcag gagaggtttc
    15721 cggaaccctg catacggttt tggatgaatt agcggactat ttggaaaaga tggaagatac
    15781 caggcgtaag gtttacagtg ctttagctta tccaattttc attgttattt tcctcttctt
    15841 tgtggtttgg ggaatgttct attacattat tccaatgttt gccgatgtgt atgccaattt
    15901 ccatgctgat cttccggctc caactacatt agctataaat atcagccatt ttatcaccaa
    15961 taatgtattt atgtttatag ttattgtgct gctggtaatc tttacctttt tcctgataaa
    16021 tcttaccgat aagggacgct atgtttttga ttccataaaa ctgaaaattc cagtaatcgg
    16081 gaaagttatt tctaattcca ttatgagtaa attttcccga actttcagta ttctgatggc
    16141 tgccggagtt cctattatgg atacgatgga actaaccgaa aatgttgttc agaatgccgt
    16201 tattgaaagt gcagttcgta aagccagagt gatggtgaaa gagggttatg gcgtagctaa
    16261 tgcttttcgc agaacaggaa tgtttcctcc tactttattg caaatgattg ctaccgggga
    16321 agaaacagga gatatggata aattattatc caaagctgca gagttctatg aaaaacttgt
    16381 ggattccgtt atagaccgct taacttcgct aatagaacca cttttaatca tttttatggc
    16441 tgttatcgta ggaagtatta tcgtagttat ctatctgcca atctttgact tgggagaggc
    16501 aatgtcccag ggactacgtt aagttaagaa aataaaaaaa aatattgtat gcggaggata
    16561 ttgttcctcc gcctttttta tagccgaaag agctttattt ttcggaaggt tggcatcctt
    16621 gttaaccaag cgcaaaaaag agttgagaag gttgagaaag ttgagagggt tgagaaggtt
    16681 tagaaggttg ggagggttta gagagttgag aaagttgaga ttccaaaaag cccgaagagc
    16741 gaaacttcta tagcgatggg tgcgtaagcc cctcggnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnntcca aaaagcccga agagcgaaac ttctatagcg atgggtgcgt
    16921 aagcccctcg gaaaattatg gcaaatctat ggtaaatcgg aatttgccaa cccctttttc
    16981 ctcaactgca ttatttgggt taaaattaga tgtataacct cttatatttt aatgccttcc
    17041 tacttgactc cgtaacactt ctgtaacact tccgtaacac ttccgttgtc tcgttacgga
    17101 attgttactt aattgttaca tagtttttaa accgtcaaga aagcaagcga aaactattat
    17161 aaatttctgt ttgaaaaagt ataaatgttc aatggcacta tatgaattcc tgcttttgca
    17221 gaattgacaa aggacttttc atccataaaa taagataatg accaaataaa ttcggaatta
    17281 aaattgccca gttccggaaa aaataacatc cctgctactt ctttttctat aaacttctct
    17341 acttatttac tcctggactt catacccttt taacttaatg acttcccgac ctcaagaccc
    17401 atagacctct tgaactcttg actttatcct tcaataggta taataattgc tttatttata
    17461 gataaggaga taaaaatgaa aaaatatttg tgtatcttgg taagtatatt tcccctttta
    17521 agtttcggag ctcctttttg gaatattcct tcttctgtaa gtcaacctga tggttcagtt
    17581 ttaaaccttt ttgccagtgg tgatgaatat gccaaccgtt tacacgatgc caacggctac
    17641 acgattatcc aaagtgctgt tgacggctat tactattatg cgattttgga aaaaggtgaa
    17701 cctgttcctt ctgcctatcg ttatggcaca attgaccctt ctgcttatgg tttaattccc
    17761 catatcaata tttccaggga agcaaggcag agaaaaattg attttatgaa tgcgcaaaaa
    17821 agaagaaatg aacggggtcc taatatcggg aatgtaaaca atttatgcat ctatatccgc
    17881 ttcagtgatc aaaccgaatt tgaaattccc cgctctgttt ataatgcccg ttttaatgaa
    17941 gttggagata atgcagtcag cttacggagc tattttcaga aagttagtta caatcaactg
    18001 caatttatga cttatcacta tcctacctgt gcggatataa ttaatctttc ttatcaggat
    18061 attcatcccc gtagttatta tcttccctat aatgcagtta ccaatcctaa tggttatatt
    18121 gatgatgata caagaacata tagagaacac aacttactta tgaatgctgt ttatgctgtt
    18181 gcctctgaag ttcctgcctc tttaaatatt gatgctgata atgatggaca cgtggataat
    18241 gtctgtttta ttattcgggg acctcattct gcctggtctg atcttttatg ggcacaccgc
    18301 tgggttttgt ttagctataa tgtttctata cgcaataaac ggattcagga ttatacattt
    18361 cagccggaag accaaaacga tgtaacaact ctttgtcatg aaatgtttca tagtgttggc
    18421 gctccagacc tttatcatta tgaatatgat ggcttaacac cggttggttg ttgggatata
    18481 atggaaagtg gggaaggtca tatgtgtatg tttatgaaat acttttatgg tcaatggatt
    18541 agttctcttc cgcttgctca aattggtaat acctatacct taaatcctgt tacatctcca
    18601 accaataatg tgtatatgta tcctattccc ggctccaatt atgaatttct tgtgtttgaa
    18661 taccggaaaa aggggagtga tgtttttgaa gagaaattac ccggctcggg actattgatt
    18721 tatagaattg atcaaaggtt tgaaggcaat gctgatggac cccctgaggg aatttatgtt
    18781 tatagaccca atggaacttt aacccataat ggactggttg ctgaagtccc tttcagtgct
    18841 gataattata gaactgcctt taatgtttat accaatcctc agtccttttt aaataatgga
    18901 aacacttttc cgattaatct taaaaatatc acttctacag gagaaaccat cagttttact
    18961 ctggcttcac caactgaatc aatggctcct gtaattagtt ctatatctcc taccagtggt
    19021 tctattttac ctgcagaaac aattcagcta aatccccaga taactgatcc cgcaaacgct
    19081 ttgttaagag tggaatatac gcttgatggt gttttagttt atacggcaaa tagcgaacct
    19141 ttttccgggg aaattaacgg taacctttta actgcaggag ttcataatat aggaataacc
    19201 gctatatcct cttcgggatt aactacaaac ctgaatgtat attaccgaat aattgatcct
    19261 aacttgcaaa attggttttc ctggatttct cctgaaccgc tttggacaga atactcgcgg
    19321 ggtgctattc caataaaagt tgccgtaaat atggatttgg gaactcagga ataccttgta
    19381 aaaggtcttc gttttaagat aactcctgat ccctggggtg aacctgatat tccgggactt
    19441 gttgttgctc aaataaatcg ttttgccaat ggggcaatta cggaacaggt tttattgagc
    19501 atcggttata tttttaatcc tggttacgat cccaacttta tatacacaat atctgataca
    19561 acaagcattt caggtcagat tgccgttata ttagaccttt ttgaatatca aaatatctgt
    19621 tttgaccaaa atgcctcctg tggtaacagc tggattactg agcccaatag aatatggact
    19681 gatgctttag gtagaggcgt tgttggctct gcaggaatag aattgctttt acaaaaacct
    19741 aatgttgctt ccgatgatcc ttttattcct gctgtaaatt tatctctttc cagctacccc
    19801 aatcccttta ccgaaaccaa taaaatcaaa tattccctgc ccgaaagcgg taaagtaaat
    19861 ctatccattt acaatattaa aggtcagaaa gttacaactc tgcaggcaga aaataaaaaa
    19921 tccggaaatt atgaactgga atggaacgga aaggattctt caggaaataa agtaagtagc
    19981 ggattatact tttgccgtct ggaaacaaac ggaaagattg ttacaacaaa actgctgaaa
    20041 ctgaagggaa tgtaagatga agaatataag attaaagagg gtttagaggg ttgagatggt
    20101 tgagagggtt gagaaggttt agagggttga gatggttgag aaggtttaga gggttgagaa
    20161 ggtttagagg gttgagaggg ttaaggggtt gagaaggttt agagggttga gaaggattag
    20221 atgccctcta ctctttgcca aaagcccctt agggcgacac aatgatagcg atgggtgcgt
    20281 aagcccctcg gaaaatgaaa gccccgaggt cttttctttc atcccatctg gaaaaataag
    20341 ccccttaggg cgacacaatg atgtagaagg tggcgtaagc ccctcgttag aatgaaaaat
    20401 gaatatccca aggaaaccag gtaataattg agaattgcgt gaaaaaaaaa gggaaaaaac
    20461 cctctgccct ttgctctctg cgttgctgat ataatagcat tctaaaaagc aattgacata
    20521 ttcaacgctt ttaattttca agtaaagaat agaaagaaat gtaattaaca taaggagcaa
    20581 taatgcctta tatatctaat actgaaagag ataggcaaaa aatcctgaag acaatcggcg
    20641 taaacaaatt tgaggatttg cttacagcaa ttcccgaaaa attaagaatg aaaaaaccgc
    20701 ttgccctatc tgagcccctt tcagaactgg aaataaataa aaatatccaa gctaaaaaga
    20761 gcaaaaatat ttcctgcgaa aaggtaaatt cctttttagg cggtggaatt tacgatcatt
    20821 ttatcccttc tgcaatagag acaattgttt cccgtcccga atttcttact gcctacactc
    20881 cttatcaggc agaagttagc cagggcacac ttcagtatat atttgaatat cagaccttga
    20941 tttgtgaact tacagggatg gaaattgcca atgctggaat gtatgatggc gcaagttcaa
    21001 tagcggaagc aattttaatg gctgtgcgta aaaatcattt aacaaaagcc attttgcctg
    21061 caaccttaaa tccgaattat gttcaagtaa ttaaagcata tactgaaggt aatggaattg
    21121 aacttttatt tgctcccgaa aaagatggag taacggattt atctgcctta gaaagtatgc
    21181 taaatgatac aattggcagt gtagttattc aaacccctaa tttttatggc aacatagagg
    21241 atgccaaggc aattgaaagt atcgtgcata aaaaccctaa atgtttactt attgcttgtg
    21301 ttgatcctgt ttctttagct atccttatgc cacctgcaga atataatgca gatattgcag
    21361 ttggagaagg tcaggcgctt ggcaataata tgttttttgg gggtccgctc tttggttttt
    21421 ttgctactaa actggatatg gctcgtttaa tgcctggcag aattgttggc ggaaccatag
    21481 ataaggatgg taaaaaagct tatgttttaa ctctgcaggc aagagaacaa catatccgtc
    21541 gggcaaaagc cacttccaat atctgttcca atgaatcgct ttgcactttg gcagcggttg
    21601 tttatttatg tttattgggg aaagaaggtc ttgttgaaat agccactcaa tccgctcaaa
    21661 aagcacatta tacggcaaac gagctctgta aaatccccgg cttcgctctg gcatatccaa
    21721 agacatattt cttcaaggaa tttgtgctta aaactccgaa gaaagcagaa gaaataatta
    21781 aagctctatt atctgggaat atctatgccg gaattgacct tgctccttac ggaaaacctg
    21841 atgaactgat gattgccgta acggaaaaga agaccaaagc agaaattgac agtttcgtcc
    21901 aagcaatgcg ggaggttgta aaatgagcaa aaccattttt gaacatagca ctgccggcag
    21961 aaaaggggta actttaccca agcgggaaat agatattcct gtggcaacaa tgtttccgga
    22021 aacaatgcta agaaccaaaa aagctcgtct tcccgaagtt agcgaactgg atgtgatgcg
    22081 gcattatatt actctttccc agaaaaatca ttttatagag aagggtttat atcctttggg
    22141 cagctgcaca atgaagtata atcccaagct tcacgaaacc ttaattaggg attcagcttt
    22201 tacctctttg catccttatc agccggaaga aacactgcaa ggcgctttgg aactgatgta
    22261 tgaactccaa aaagacctgg ctgaaattgc cggaatggca ggtgttacat tacaaccctc
    22321 tgccggagct caaggtgaat ttaccggtat caaaattgtt tctgcctatc ataaagccaa
    22381 aggaaatacg cataaagaca caatcatcat tcctgatagc gcgcatggaa ctaatcctgc
    22441 cacctgtagt ttggtgggct ataaaacagt ggaacttgcc tctaatgaaa aaggtcgttg
    22501 tgacattgcc aaattgcggg aactggtaaa tgaaaataca gcgggaatta tgattaccaa
    22561 tcccagtacc ttgggacttt ttgaaaccca aattgaagaa atagctgata ttattcacag
    22621 tgttgatgcc ttaatgtata tggatggagc taacttaaat gcccttttag gtatagttca
    22681 gcccgggaaa atcggttttg atttgatgca cttcaatttg cataagacct ttgccacacc
    22741 ccatggaggg ggaggtcccg gttcaggtcc tgtagctgta aatgaaaatc tactgccttt
    22801 tttgcctgtt cccgttatta ctaaagacga aaagggctat catctttctt ttgcaaatga
    22861 aaagacctcc attggcaaag tgcatagttt ttatggtaat ttcgctgttt taatccgtgc
    22921 ctatatctat attaaaatgt taggagcgga aggtttacaa agggtttccg aaaatgccat
    22981 attgaatgcc aattacctta tgaaacgctt gaatccatat tatcatattg aacatcagga
    23041 accctgtatg catgaatttg tgatggatgg aacctggcaa aagaaggaat cgggaattac
    23101 tactttggat atagccaaac gccttttaga taaagggttc catgcaccta ctatatattt
    23161 tccgctcatc attccggaag caatgatggt ggaaccaaca gaaacagaat ccctggaaag
    23221 tttggatgcc tttgccactg caatgataga aattgctgca gagtgcaaag aaaatccaga
    23281 acttgttaag gaagctcctt taacaactcc tgtaagaaga gtggatgatg ctcgcgcagt
    23341 taaaatgctg gagccgattt ttccacttca ggattaactt tatacctttt tcagaagagg
    23401 agaaacaatg aaaaaatata tcttaactct atcagttctg ttagcactat ttgcttttgc
    23461 ttatgcccaa cccgttaagc ttggttttgt aaataccgac cgtctgctct tggatagtaa
    23521 cgaagcagcg gaagtagcca ggttatttgc gttggataaa cagaactgga caaatcaaat
    23581 taaatcgctg gatgaagaaa tcaaacaaat ggagcgcgat tttgaaatcc gcaaactaac
    23641 gatgaacgat gccgctaaaa gagaagctca aagtaggatt gataccaaaa aagcggaagc
    23701 aggtcgcctt ctggaagaat attttggtga tgggggaaag gcggaacagc gttatcgcga
    23761 acttattgat cctttaacct tgaaaatcca taacctcatt aaaaaaattg ccgaagacga
    23821 aaaatacaca atgatttttg atgttagtat gggcagtgtc ctttatgctc ttcccgcttt
    23881 ggatttaact gatcagattt tactggagct gaataaagat acgattaaac caactgaagg
    23941 aacaacacca gaacctccca aaccggaagg tcaaaaacct cctgaaatta agccacctga
    24001 gggtttcggt gatgaattta agccggataa attatgaagc gttttaacat tcctataaat
    24061 agtgaacttc ttactcaaat ctgccaggga gaactgaaag gcaaaatacc atctgtcctt
    24121 tgtaaagtta gcgaaccgga agaagcggat gaacaaacaa ttattttctt ggagcaggaa
    24181 aaatttctgg aaaaagtgaa agccagttct gccggcttga ttattactac ggagaaattt
    24241 atttccgagc ttccggaaag agctttactg cttgttcaaa agccctatta ttcactaatg
    24301 cgattgatta gctggtggct ggaacaggaa aataaggact ttatttatac aattcagtca
    24361 acagcaattg tggcagaaga tgttcgtttt gaaggagaag tggcaatagg agacaatgtt
    24421 gttatcggta gtggttgtat tttaggcaag ggagtgatta ttagcgaagg atgttctctg
    24481 ggaaaaaatg tttctgtagg agcgggcacc aagctctatc caaatgtttg cgtttatgat
    24541 gattgcgtta tcggcaggaa ttgtattctt cacagcggag ttattatcgg tgcagatggt
    24601 tttggtttta tgctaattga gggtattcag caaaaaattc cgcaggtagg aaatgttgtt
    24661 attggcgatg gtgtggaaat tggagctaat agttgtattg atagagctac tttaggcagc
    24721 actataatcg gcaatggcac taaaatagac aacctggttc aggttggtca taattgcatt
    24781 attggagagc atagcattct ttgtgctcaa gttggcttgg caggaagcac cgttgtaggt
    24841 gattatgttt atcttgcagg tcaggtagga attgccgatc acttgcaaat agggaacaga
    24901 gctatggttg gagctcaatc aggtgtttcc actaatattc ccgatgacgg aaggtatttt
    24961 ggctatcctg ctctggaagc gaaccttaca aagcggatta tggctgtgca aaagaattta
    25021 cccgaaatgt atcattttta cctgaaggca aaaaaacaag aaactgagaa cggagaaaaa
    25081 taatggaata caaacaaaca attggcggag aatcaactta tacaggaatt ggattgcact
    25141 ctggagaaat tagtacgatc agattcaaac ccgcaaaagc agatgaagga attgttttta
    25201 tccgaaccga tttacccgga aaacccaaaa ttcctgcaga tattgatcat gttgtagata
    25261 tttcccgggg cacaacaatt ggcattaatg gcgctaccgt agccactatt gaacatgttc
    25321 tgtctgccat tactggaatg agaattgata atatctgcat tgaaataaac ggacctgaat
    25381 cacctgtggc agatggttct gccgttgttt tcttaaatct tttacggcaa tgcggaattg
    25441 tgcaacagga tagtgaaagg gaatattttg agctggattc gcctatcagt ttttccgctc
    25501 cggaagataa tgtggatatc gtaattgtgc cttccaatga actgaaaata acctttatga
    25561 ttgattacaa acatccctat ctgggaacgc aatatacctg gttgccttct ttggataatt
    25621 atgaaaagga ttttgcagga gcccgtactt tttgttttat caacgagatt ctggaactga
    25681 aaaatatggg tttaataaag ggtggttctc tggaaaatgc tttagttatt gccgaaccta
    25741 atatcagcga agcagaattg aagcacttgc aggatatttt tggttatcat aaaccgatta
    25801 ctgtttctca ggaaggtata ttgaacagcc atcctttaag gtattacaac gaattcgtcc
    25861 ggcacaaagt tgttgatttg attggcgata tagctctttt gggagttcct attaaagggc
    25921 atattctggc agcgcgtagt ggacataaaa ccaatgtgga actggtgaaa aagttgcgac
    25981 aaatacagaa aaagcaccag ctccaaaaga tataccaaaa agacaaatcc caaaatgtgg
    26041 tctttgatat caatgccatt ttgcggattt taccacatcg ctatcctttt ttgctggtag
    26101 ataaaattat tgaattcatt ccaggagagt cacttgtagg cattaaaaat gttactatca
    26161 acgaaccctt tttccagggt cattttcctg gtcatcctat tatgccagga gttctcatta
    26221 ttgaaggaat ggctcaggca ggtggaataa tgttacttaa tcaaatggat aatcctcaaa
    26281 attatgttgc ttattttgct agtattgata atgtaaaatt tcggaaacct gttttacctg
    26341 gagatacact gcgttatgaa ctgaaggtga tttccctaaa aaggtctttg gcaaaaatgc
    26401 acggagaaac attcgtggaa ggagaaaaag ttgctgaagg tgactttttg gctatgatta
    26461 tggagagaga aaaatgacta ttattcaccc tactgcaatt atagaagaag gcgctgttat
    26521 tggcgataac tgttacatcg gtccctattg tcatatcgga aaagatgttg tattgggttc
    26581 aaataataac ctgattgcca atgttactat tttgggagat acaattatag gagacggcaa
    26641 taaaattttc ccctacgctg ttttgggaac tgaacctcag gatttgaaat ataaaggtga
    26701 accaacccgt ttgcgggtag gcagtaacaa taccattcgc gaatttgtta ctatcaattg
    26761 cagtaatcaa atggaggagg ataccgttgt tggcgataac aaccttttaa tggaatatgc
    26821 gcatattgct cacaacagtc aaatcggttc cggttgcgtt attgctaatg ttgttcagtg
    26881 cggaggacat attcatattg gtgattttgc tacggttggc ggtttaacgg caattcacca
    26941 gtttgtccat attggtgctt atgcctttgt gggtggggct tcggcaacaa acaaagatat
    27001 tgttcctttt tctcgcggac agggaaatcc ctataagaca gttggactga atagcattgg
    27061 gttaatgaga aagggctttg ccagcgagac aattgccgct atcaaggaaa tatataatct
    27121 cttttatcgt agtggattaa atacttctca ggcattggaa aaagcacttc aaataccaaa
    27181 tcctacacct gaacagataa tctttatcca gtttgtccaa aacgcccaaa gaggtatttc
    27241 caaataatat tgaacttacc gggaaaataa tgcctgcggt cgttcagtat gcttttatac
    27301 catatccgta ttcataaacc caactaatac agttcagaat aaatggattg gcaaattgga
    27361 aattgtcagc ccattttcct tcaactgcat tatgggagtt aatcccgtta aaagatagaa
    27421 tcttacctgc ttgcaactca tcctccacct atcctttagg ttgggttgat gatgggttat
    27481 aattaaggat gaggaaaaaa tataccctcg ggctacacaa aaggttgagt tttaaagatg
    27541 tcaggagtcg tcactgccca aataactttt accctttcca caagtttatt tggcagggag
    27601 gacttatgac cgttcccaaa aaaaattaag cttccttaat catctctcaa taaaatactt
    27661 aggcacacta taagtccctc accaaaaata ttttttccga tttctgcatt tttttcttgc
    27721 ttttttcttg tttcggttta tattataaat tgcgttacca agttttggga gcagcggcaa
    27781 agaattataa agttgacttc gagtcagtat ctaatattgc attggctgct ccctttattt
    27841 ttgttccttt tttagtttag cccaccagtc aatccgtttc tgaatttctt tttcaaaacc
    27901 gtaagttccc ggtttataat atatcttttc cggcatctgt tcggggaaat acttctggta
    27961 ataatactta tattcatatt cgtggtcgta atgataatcc ttaccataat tcaggtcttt
    28021 catcaattgc gtgggcgcat ttctaatatg caaaggaaca ccgtagtgac ttgtttttct
    28081 ggcatcttcc gctgcttctt cataagctgt atagagggca ttacttttag gtgcagtagc
    28141 caaataaacc accaattgag ctaaagcagt gcttgcttca ggcattccta taaaaagaac
    28201 cgcttccttt actgcaattg cctgaaccaa agcattcgga tctgccaaac ccacatcttc
    28261 gctggcaaaa cgaatcaaac gacgcacaat ataacgaggg tcttcccctg cttccaacat
    28321 tcttgccagc cagtaaagtc ccgcttgcgg gtctgaacct cttagtgatt tatgtaatgc
    28381 ggaaattaag ttataatgtt cctcgcggtt tttatcgtag aacattgtct tttttgccag
    28441 ggattttgcc agttcctcag ttttcggatg cggtttcttt tgcagataag gcaaaaccag
    28501 ttccaaatcg ttcaaaaccc ttctggcatc acctccggca ttttgtgcca tccagtttat
    28561 aacttcttca tcctcttcca atttcaattc tgcaaaaccg cgcttcaata ttattttcag
    28621 gtcattttcg ctcaagggct ccaacacaaa aacagtgcac cgggaaagca atgccggaat
    28681 cacttcaaaa gatggatttt ccgttgttgc tccgattaaa attatggctc cactttccac
    28741 atagggcaaa aaggcatcct gctgactttt attaaaacgg tgaatttcat caatgaacaa
    28801 aatactgcgc ttgttttgcg tccgatgcag ataatccgct tctttcatca ctgcttttac
    28861 atcactgatg ctggcaagaa cagctgaaaa acggataaaa ttgtaaccgc tgtttttctc
    28921 tataatcctg gcgatggttg tttttccact acccggagga ccccataaaa taaaggaatg
    28981 atactctcca ctttcaatca tctgccttaa aggcgaattt tcagctatca gcttcgtttg
    29041 acccaatagc tcttccagcg tttgaggacg taatttttca gccagcggaa ctgtcttagt
    29101 gcttaattct tctctatcaa aaagagtgct ttgatcttta tttgccatta tctaaaagct
    29161 caatcctcca tcaatccgga taattcggaa ggatcaaacc actgtaaagc accctgcaaa
    29221 cgctgaattt ccgaaagtaa aattgacttg tgaccttctt cttctttggc aaattgcagt
    29281 agttgtttct taatttccgg ttccctggtc tgctctgcca tatttttata gagctcaacg
    29341 gattcttctt ctttggtgat ggcaaattcc aagacctcct gcggatcatt ccaatttaca
    29401 ccttgcattt ctttaatcgg ttcttcctgt agagttattg tttgcccggg aaactctttg
    29461 gcaaaagcag accttacctt ttcttcgtgc attttttcta aaacgaccag ttccttgaat
    29521 acattgctgg tttcgggctt ggcaaaactc tttgctaatg cctgatacat cttttgtgct
    29581 ctgatctctt ccgctatcac catccggtaa ctatcttcta atgtcatttg tttcacctcc
    29641 ctgttaataa cattataatc ggattgggca aaaaaagcaa acgggatata atgcgaagtg
    29701 gagaggtgga aaacaaagag ataaagagga aaaaagacaa tattaacaaa gagataaaga
    29761 ggaaaagaga aaatattaac aaagagaaaa agagaaagat gaagtaagtc aggagtcctc
    29821 actgcctaaa taacttttac acatcccaca aactcatttg gcagtgagga ctaatgacga
    29881 ctacttattt cacgcagatt tcgctgatta cgcagataac aggattgatt ggatgagcag
    29941 gatttataat tattaattta taatctgttt gaatcaatta aatcaatgtt gctattctat
    30001 tatgagtcat ataccctgat aaagttatct ccaagagaaa aataatgaag gcgtaaatgc
    30061 ttcctgatta aaagttaact tgtctggatt cctgctttct cgggaatgat aaaaggggaa
    30121 aatgatatta gacctaaaga cctcttgacc tcaagaaacc ttgacccttt gatttctcac
    30181 cttttcgcta ttccgtaatt ttttgcttga cagaaatggg gt
//
LOCUS       DAOOZV010000018        28777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1777_length_28777_cov_53.393601, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000018 DAOOZV010000000
VERSION     DAOOZV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 28777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28777
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_28777_cov_53.393601"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            521..5338
                     /locus_tag="PLW08_03225"
     CDS             521..5338
                     /locus_tag="PLW08_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL51647.1"
                     /translation="MSYNMNKAQSDDSSIYKMALDWSCSIKTIFIIFFILCLSTLTWA
                     QQSPNGYTYQNDWLYYSYFSQTNNGGYTQIDHINIQMNTSGNTITPETIQVNTDNVVI
                     HTNINLGIGAGRYILKFWVSKQINGEYVNQGSYVSQFSYMHQTNYFTVPAVGNGSFVL
                     SFDLYNLYGQHVHSGYHVFEKTISQQQVLINHNRDVLFKMRSSNSSLKRPMLLVEGFD
                     PLNSQSFDYYNEKISQMENGNAGVFDNVDLYLLNFRDATIDLRENSNVVIGAIKYINT
                     LYPQETLMEGISVLGFSMGGVISRYALAYAEEHEIPHNCTQLITYDSPHRGAVINTNL
                     QDELYHIYDTIDNLQEAVSAIEWASLILPLPGGLIISMAASDFNNTLLRYKAMFQPIL
                     KTIEAPASRQLLRYNINSDGYTDELNGSIDYRDFYSEINAEDRLAYFPNTVNGVLNKD
                     VNDPNNKPGYPYKQNAIKSIAISNGWITKSGDISDSFNNICTFNLPSIWGNTHINEQN
                     WDTQPGSTLGMESWAGSANSFLDIHYDPVFIPLKSSLHLRPTGSNGFPDNADLVMNIY
                     DDIVPRDNLNNIITDYQEITDYLSSHSLFDSVVLSNVANLRHADISDFVVTNAVSNFR
                     NPFNRAIGIISGTITNSDLTGIEMKVYIGDSELQLSQDFYRLNPDGSWAVAYTLLRDS
                     DVKIRFGKPGSLPTIRKYHIDYDNTSYSINGIDNQQVSLKDFNLRNIIVSQANTGSFE
                     EINDALRFIVEHFSENVYNGEAIRIIVLPGTYTESVDLSPLAALGITNFTLEGVGDAI
                     IDGDGYGINLVVNAYSPCIGAVYNIRKLKIINSDRGILFKDYWDESSGNAQAPQLTLN
                     INNCTIYNCGSSSYSGTSDPIFSAAAIHFEGAGTISYCNISNNNMLSGSSTYSLYIQA
                     GGVYISNNTTSLAEVRYSKFTSNKGGLSGGLVAKGKGEIWIQYNEFTGNERRGPCSVY
                     NAYNSNAMTVYDASNTLINNNLFVDNIQSIIPYGAVIGLNTYNTQAAAPIRFVNNTIV
                     NTPLYNNNGLCAIGFRINAGVAIQDIHIKNNIISSTNSNGCTFDSGAGYSPVSIKYNI
                     LHNITLSGFTANLYNPDDPNSVYNPTAPRFNYQCDPQLDANYIPAWNATTMSHCIDTG
                     TGVYDPDGTPPDIGAFRATDHRYWQYCFRSGQNDRSDTYHWVSYPVVNSLTQGKTIAN
                     VFFKELLGKHIELINGVPQWKPDVLQEILWYEGNESRYIVLEDSDWGPYVDTHNVVSP
                     QGYKVKLLPVANNGLTPTTVELHHSGFLTPENNPFMIYGSSSNGGQTYENWIGYFDTD
                     SVWPTDAFASIWNDITMIKAKNWCLYRDPNSGIGALIGKMLPINCGDMVIVTTVNDHE
                     FQWKKTIPTDPKKKEVPTAFEYVEKADYTPVYIDLSAIDLSNLKEIGLKLDGICKGAV
                     VVTDSLEQICAYLEDGESLSSGLVELVFFYESKSQPLEMRSVAIKDKQLSSSIIGGDS
                     AYPVYNIKVSTADLSNSVVPVLSLEQNYPNPFNPTTSIRYSIEEPGSVTLDIFNIKGQ
                     LVKTLYHGNAEIGSHTVIWNGLDNSGNACSTGVYFYRLRTPKTSLVRKMLMLK"
     gene            5395..7767
                     /locus_tag="PLW08_03230"
     CDS             5395..7767
                     /locus_tag="PLW08_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51648.1"
                     /translation="MKRNRWIIAGLLCLIGIGMHAVTRIVDISGAGQYTSIQTAINAS
                     SSGDSVLVYPGRYYESVTITTNDISLFSREYHTNNHDYINSTIIDGSGNSLCLRILSY
                     TQNVLVRGFSLTHGRGVLLYPNSLSRFENCNIFGNSSLDGAGINVSMGSVYLSGVKIY
                     NNDAYNMGGGMYINGYMGSVSVTFDTVNRCSIYNNTAGAGQDIVAHSINNDLSIPLDM
                     FTVANPTSYYATSLRAAGDEFQLLINAQRYHHQEINNDLYVSPTGDDTNDGLSPATAL
                     KTIRTAVYRVATDSLNQKTVHILPGTYSRTANQQVFPIPLKSWVKVQGAGIEETQIVG
                     EMDPAYANVQYNALKVFTSFYQTHTSLEDLAITSAGSNNSCAIWGHKEESLHLKNLRM
                     HELNPDDNAVIHIRTPINTVWDNVIIENIITARHGLVACQAGMTGIVKNSIFRNAANT
                     FESLEVWGYPLIWVSAGESITVENSIFTNLSMADDDSQALSFGGVSAPTFQQYYTFQN
                     CLFSNINCNVRGVLIVGHTYPEMNITNCTFAGHTGNGEALMVNGNVTISNSIFYNDRW
                     VEIAINPMDGTGIPTTLTLNNNLIRNGYSDIWQGPGSTIHYSDTNITGNPFFLGGDDI
                     NNPLYYSLSAASPCVNSGTPDTTGLCLPPYDLAGNFRVWNNRIDIGCFEYGSEPWVSN
                     DDPVAPDIPAVSITAYPNPFSVFTNIKVSTLNDGSDRTESVNNVSIAIYNIKGQRVKS
                     IILDPMKTSEQFTYWDGRDKDNIRCSSGIYFINLIVNGRNISTRKVTFIR"
     gene            8732..10180
                     /gene="tilS"
                     /locus_tag="PLW08_03235"
     CDS             8732..10180
                     /gene="tilS"
                     /locus_tag="PLW08_03235"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPL51649.1"
                     /translation="MTIAEALKRLEKNIKENELIHNGDKLLLGCSGGADSNAMLYLFS
                     RLRVPLNLTLLVIHINHQIRGAESDADEDFVKQLCQDLNIPVIIRKIVVPETGNLENQ
                     ARKLRFEVFNKILESYNFDKILLAHQKEDQAETVMLNFLRGAGISGMAGIKGITGKVV
                     HPLLCFSRKELEEVLKQAGISWRTDSSNLETKFRRNLLRNEIMPQLEKEFNPALKSHL
                     FFLAELFSQAETIFKQRSKTQFRRICIDIQPGKVVFELTPLLKLSPIERYYLFRSAFQ
                     YVCGSENDFFAVHFQAIENILNGKGSKCLNLPHKITVIKQYDEVIVQIETEKEVKETE
                     PLVIDADRTMAVYGNYRFNFKYLRVLPKEQTGDELESRVILDADKINIPFIIRFRQPG
                     DQFVPFGMTQLKRLKEFFIDEKVPKYERDLIPVFDDGEKLFWIVGYRLDNRVRYDEST
                     TRYLQITATNISVKSRKAILKKKRGNNESDEL"
     gene            10161..10709
                     /gene="hpt"
                     /locus_tag="PLW08_03240"
     CDS             10161..10709
                     /gene="hpt"
                     /locus_tag="PLW08_03240"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPL51650.1"
                     /translation="MNQMNCDISAVLFDEYQIQTRIRELGLEISNSYKNSTPVMIGIL
                     KGGFIFLADLVRSITIPVEVDFLAISSYGTKTSSSGVVKIRKDIDIDITGRDVIVVED
                     IVDTGLSLAYIKDYIWQHKPSSLKTCVLLDKPEAHKTDVTFDYIGYSIKNEFVVGYGL
                     DFAEKYRNLPFIGILKEELYKC"
     gene            10703..12937
                     /gene="ftsH"
                     /locus_tag="PLW08_03245"
     CDS             10703..12937
                     /gene="ftsH"
                     /locus_tag="PLW08_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPL51651.1"
                     /translation="MLKYILISLCLITFSALNAIPADTLITPQQTIPEAGKAIMQFST
                     LMNWFILALLIISVISIIRMIIVKKRTLNRKDKPNPNPNLRNPQSPKNAPPKKSPFSL
                     PLIIMLILLIVIIFHTYNSSGTAVTKESYSNFMTRVANGQITQVQFAEKDIFYTDIAN
                     KKYSTTMPYENPQLVDSLVALGIKVSATKPSRWAGVISYLLPFLLLIIFWVFFIRGMN
                     AQNAKAFSFGKSKARLYEASKTGITFKDVAGVDEAKEELQEIVEFLKDPKKFQRLGGR
                     IPRGVLLVGQPGTGKTLLAKAVSGEAGVPFFSISGSDFVEMFVGVGAARVRDLFDQAK
                     KNAPCITFIDEIDAVGRHRGSGLGGGHDEREQTLNQLLVEMDGFEPNEAVIIIAATNR
                     PDILDPALLRPGRFDRRVVVDLPDIKGRTEILKVHTAKVPLANDVHLELIARGTPGFS
                     GADLANLVNEAALIAASKNKTQIQMEDFEQAKDKLLLGKEKKSRVIPEEDKRLTAYHE
                     IGHVLTSVYLDKVEPVHKVSIIPRGFTGGATHYLMTDKTNYSKSYLLQMLVTLLGGRA
                     AEEVVFQEYTTGAGNDLERCTDIAKKMVCSWGMSDKIGPITIGKEQGEIFLGKELVSH
                     DIFSEETSQLVDREIRDIISNAYSQAISLLNEHRELMDIMAKELQEKETLGTDEIFNL
                     ILENCREEERELVEEKYKKALELRFEHSEKTTSQPTETTETAETTETVEQTENKDNKS
                     EETA"
     gene            12938..13468
                     /gene="hslV"
                     /locus_tag="PLW08_03250"
     CDS             12938..13468
                     /gene="hslV"
                     /locus_tag="PLW08_03250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPL51652.1"
                     /translation="MTMHGTTIIGIHRNGKTAICGDGQVTMGDAIVKGKAQKIRKIYE
                     GKVIVGFAGATADAFSLLERFEEKLKTNKGNLKKAAIDLAKDWRQDRILRRLEAMLIA
                     GDKDSILLINGAGDVLEPDDNIIAIGSGGNYALAAARALAHKTKLGASEIAVEALNIA
                     SGICIYTNNNYIVEEI"
     gene            13470..14834
                     /gene="hslU"
                     /locus_tag="PLW08_03255"
     CDS             13470..14834
                     /gene="hslU"
                     /locus_tag="PLW08_03255"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPL51653.1"
                     /translation="MLINEYLTPKRIVEELDKYIIGQDQAKRCVAIALRNRWRRQQIS
                     GDIKGEIMPNNIILIGPTGIGKTEIARRISRLTNAPFIKVEASKFTEVGYVGRDVESM
                     VRELMNYAVSQVREAMIEEVRPEAEKNATEMILDILLPRSKKMKQNANNTDTPEYERY
                     LRSREKMRKKLLSGSLDEKEIEVEPETDNLPSFEIFPGPGLELLDFDLSEMFSNIFQG
                     KGTPRKIKTTVHTAWKRNVENEINRLISKDKLVETAKIAVEENGIIFIDEIDKIANTD
                     KQVGIDVSRSGVQRDLLPIVEGSKVPTKYGMIDTSHILFIAAGAFNTAKPSDLIPELQ
                     GRFPIRVELNSLTQKDFERILTEPENSLTKQYQAIFRSEGVELKFMPEAIREIARFAA
                     LANEKMEDIGARRLHTVMNKLLDDFLFEMPSETLKSVKITKKMVTEHLSPVIESEDLS
                     RFIL"
     gene            14837..16147
                     /locus_tag="PLW08_03260"
     CDS             14837..16147
                     /locus_tag="PLW08_03260"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL51654.1"
                     /translation="MVINKIKEIFVSLTIRNYRIFFIGQGISLIGTWIQRTTMGWFVY
                     RLTGSAFLLGLVSFLSMIPSVFVSPFVGAWADRLNRHRTMIHTQIAFCLQTGILALLV
                     LTRVINKQVWYPILILSFIQGIIDAVDAPIRQNFVMDLVSSRTLLPNAIATNSAMFNG
                     ARLIGPAIGGFLIMMFSEGVCFAINAISYIPVIISLCFIKISYPPVPPQKEPTLKKIF
                     SGWKYSWENMPIRFLLSNLAVFTVFGMSFSTLMPIFAKDILHGTSATQGLLMSSMGVG
                     ALCGSFFLASRKNIRGMPSRLVYLCLTFSIACILFSLSGSIPLSMFFMVFLGLAGMMT
                     MATTNTLIQSISSLEMRGRVISLYTMASVSMSPLGSLLMGTLSSRIGARFTLIVCGLV
                     LLLWSLNGLRIVPKFLRGVLRMLIISDNTDVYRQRVLAPEISAR"
     gene            16144..16833
                     /locus_tag="PLW08_03265"
     CDS             16144..16833
                     /locus_tag="PLW08_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPL51655.1"
                     /translation="MKLFYYLGKREPDYRTLMHFQKDFMLEGIKIPVSRLVIAEQTHS
                     KEVHICREIDCGAGIGNKPQIPLADGLITNIPYQYLLIRTADCYPILLMDSRRNVVAG
                     LHCGREGTQKNIVGEAIRKMKSHFYCQLTDIIAIVGAGICHKHYEVSQELFDNFNRAL
                     RKMGLEPDITQNRHLNLRKILNAQLIKEGIPEINIENVNDCTFESSNYHSYRREKTNN
                     RQINIVGILYG"
     gene            16826..18118
                     /locus_tag="PLW08_03270"
     CDS             16826..18118
                     /locus_tag="PLW08_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPL51656.1"
                     /translation="MAKTYVNKEGNLILEIREEPLSAWLTIKKTDFLIDENEILALIE
                     EAGIKSGFDEAIDYICKHSLEKEFEVPFPIAMCNKKEVTSMLRYNFNPDLLSRPENGI
                     NISTLEKLKVFRSGDVVAEYSSNIFAQGGSIYDIFGNLLDANSVDTEQAKALAGDNIA
                     YNVQNKQFSALVDGFPYLDENGCICLLDKVLLNGNEIPPETKVKCPINLIIEGSITYA
                     DIHCEADISVQGDIQFLTINCAKNMFIAGDIISSNRKGIIVWGNLECRSILNSYVLCL
                     NNIHFTDKIENSTVVCDGEIIGDLNSSEICGGLTQAGESITVFQAGSYDRTKTEIEIA
                     LCPFYRNLLLLLTRELIHLKEDKELNATTIAELENRIEKYERALDEKLNQILKSEKQK
                     PKTIKVQDVIRPPVVFRILKHSYEVNRQEKGLEFEEKD"
     gene            18247..21285
                     /locus_tag="PLW08_03275"
     CDS             18247..21285
                     /locus_tag="PLW08_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL51657.1"
                     /translation="MCYVYSNDLWLVPFTGGIPRRITNTTSYEWNPQFSPDGKMLAFS
                     SDRSGQNLIYIMPATGGEAKAVNYESFRISEWFSDSQNLLCTKYSPRWGLSFYKVPID
                     GSRPILLAEIGSSFATLSPDNKQIVFQKGGYSRREAYRGSGNGELWLIDLPTKKYTKL
                     TNTELSELYPRFSQTGALYYCASDGNCFQINKVNKLSFRKPEKITNLKPFSARRISLA
                     RNNDRLVFEYFNTIYKYDPTLKTGEKVSPLFIDLCEDEWQENIVRSYENTTIDDYTIS
                     TDELLVGFTHNYDSFFAPVKGGEAKQISFDHGSIFGLQFLDKRQMVISKLDNGIVKLF
                     TATADSVITLSPVDWFGADSLCVTELYKDKNGRWYFKYDDYYRHQKIAIADSGFVNIR
                     PVNTPWAVTTNFAFTEDGRYAVYGTLREDNYVKELYLYDVQADSSKRLLADNGWWQNL
                     FWTSDNKSILLTRNNDIYRLDLVPRDEFELDTDPWDEILHPEKSKADSTNEERQNEEN
                     GIQEIESTWKEKKEEKPKNLEIVWEGLEKRFYPVITAKDNYNFVLDIVSDSTFYYIEN
                     NRQQEKNSILKKANIYGKNQKEVFNFGRNAGYYNKVNNTVYYLINQELKSYNLDTSAR
                     KDIKIKYDYKYDKALLNLRVFEEVWCAFGNNFYDPDMHNVPWKELFQLYRPYVEKAKD
                     IYDVAFIIEEMIGDVNASHTGFYPREEYKPFTNPVAWLGADFDYKDILAEGIRLERIY
                     PTSRLSSFYHLQDGCILTHIDEVKITAHTSLDSLLAGKIGKKIKLTILQEGNITEAVI
                     TGLSYSQMSELKYDYQIAKNKKLVDELSNGKLGYIHIPAMGENDWQKFYRDFVVDNFD
                     KDALVIDVRGNYGGHIHDKLITLLQKKKYAYTTSRRYSRVMCYEPHNIWDKPSIVLVN
                     EDSFSDGEIFPTVYQELKLGKVVGTPSSGAVIGTWHYYLQDGSSMRMPGTGWYKLDGT
                     NMEGTGVQPDIVVENLPEDIIAERDPQLIRAIKELMKELK"
     gene            21289..22164
                     /locus_tag="PLW08_03280"
     CDS             21289..22164
                     /locus_tag="PLW08_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51658.1"
                     /translation="MNLNEINNLIQRASALVKTNWLQAVYILQEALKEEPDNLSLLVN
                     LGDIYLERQLYERALSYYQKAISLLPDNPQLLFLIGTCYFSLGEYRIANSYYNRIPDP
                     PPEILYNIALSYAFLGSYQESIDIIHKILKVMDDNPFIYFLLIEQYVRIQNYDRAYEI
                     ILTAENKFGKHRQLLLLSALVYGKKGIWLKSYHCFVEYESLGEITNPDHLMAYANAAV
                     NIGMNDRAIELLQRAQEINPYINAVYEELIRLYLKRNDYKNAKKVLDTAKRYISRFSP
                     VLYLFKERLDNSHLE"
     gene            complement(22786..23205)
                     /locus_tag="PLW08_03285"
     CDS             complement(22786..23205)
                     /locus_tag="PLW08_03285"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPL51659.1"
                     /translation="MKEKSLLLIKPNAVRNKHIGHIITILEENNFHFCALKIFRFNRE
                     LAEHFYAEHKGKSFFEKLLNFMMSDFTVAIIVQKDNAVQELRDLIGDVNPEKRKPGTI
                     RYLYAEGITENAVHASDSIDSAKREIGIIFNTENTDK"
     gene            complement(23202..23525)
                     /locus_tag="PLW08_03290"
     CDS             complement(23202..23525)
                     /locus_tag="PLW08_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51660.1"
                     /translation="MLNHEDLQINYENLLKLGYVVVDIRYKEYDICQETPKLVIARVD
                     SDRDDFYQDMLKLYTGIEFKPNEMYEIWTDILKHKIKMSMVLNRDIAIKVAALDFIET
                     VYTAK"
     gene            23651..25654
                     /gene="uvrB"
                     /locus_tag="PLW08_03295"
     CDS             23651..25654
                     /gene="uvrB"
                     /locus_tag="PLW08_03295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPL51661.1"
                     /translation="MQRFKLISDYTPSGDQPEAIEALIKGIEEGKKHQVLLGVTGSGK
                     TFTIANVIAHFNRPTLVLSHNKTLAAQLYGELKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDSSINEQIEKLRLRATMSLMERRDVIIVSSVSCIYGLGVPEEYREALISL
                     KKGMKMDREELIHQLISAHYSRNDIAFERGAFRVRGDVVDIFPAYLEHCLRVEFFGDE
                     IYRLEKIHPISCQPLEEVENYDVYPAIHFLMNEEKLRKALISIEQEMNERVAWFLQNQ
                     KYVEAERLKQRTIFDLEMLRELGYCSGIENYTRHLTGAKPGEPPNCLLDYFPEDFLMV
                     IDESHVTIPQVHGMFAGDYTRKKNLVEYGFRLPSAFDNRPLRFEEFEKFMHQVIFVSA
                     TPADYELSLTKGEIVEQVIRPTGLLDPEIEIKPIKHQVDDLIAQIKERVAKKQKILVM
                     TLTKRMSEDLSIYLNNAGIKSRYMHSDIDSIERAKIIRDFRLGEFDVIVGINLLREGL
                     DLPEVSLVAILDADKTGFLRSTRSLIQISGRAARNVDGKVIFYADEITDAIKQTVAET
                     NRRRAKQLAYNEEHGITPQTIYKTIDQIMQSTAIAEGYDKIAKQATFEKPSAKEFMEY
                     LELDNKEKIIELLTKEMNKAAANLDFEHAAELRDKLWELQQNI"
     gene            complement(26571..27206)
                     /gene="udk"
                     /locus_tag="PLW08_03300"
     CDS             complement(26571..27206)
                     /gene="udk"
                     /locus_tag="PLW08_03300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPL51662.1"
                     /translation="MSQTNNVRLILIGGGTCSGKSTIAKAIGMRLNDLKTVIVAQDNY
                     YKDLSHLPPAMRAKVNFDHPEAIDIPYLLSDLKLMLEGKAVDIPDYDFATHCRKEGKT
                     CVAFADVIILEGIFGLYYPELLALSDLKIYVDTDSDLRLARRMQRDIVERGRDVESVL
                     EQYLSTVKPSHEAFIEPTKKNADIIIPGDREFDNVLYMLNGYLLYEFIMER"
     gene            complement(27190..28425)
                     /locus_tag="PLW08_03305"
     CDS             complement(27190..28425)
                     /locus_tag="PLW08_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51663.1"
                     /translation="MCAGLDRVGSIKNSERKKENMVMLIKKVLFIDEPFTAEEGIRAE
                     RSRFLWNVISKSFDADLLLLKSPVYMEKPVSLHTGYDKLYSLSLRDENNLESESYHLI
                     GKGQKERFINILDSKRYEVIFFAGLACLPLVCLAKKILHQCKLIIDIEKVWLPEIKEK
                     WKNNKQLKNANYLWQYIRQTAWNKYLPKKGIFCLYANPGETDELFKCYHFKPENTHFV
                     PLPITTIPELDKPGNNKKFILFWGAPESKDNLTAVKNIISEIYPRISKKMVEKEISIV
                     LCGGDELQGLCSGRITYAPYSEKEQLLQEALLVLLPLNKPDTEGRILTCAQFRKTLVC
                     TLSTIKNFPLPEDSYLASDDFAELSEKIIELFRSAKKLDEYANKLSTFCFANFTPEKV
                     ENALLTKINSWIGNNVSNQ"
BASE COUNT         9047 a         5297 c         5900 g         8533 t
ORIGIN      
        1 tgataaacct attcctctga ttcctccaat tattgttgac aattatatgc atctgatagg
       61 aaaggatatt aagggggata ctttttacct ttaccaccca gcttattaaa aaaaagaact
      121 tgacaaggat ttataagata aaaagaatgc aattggtctc ttggcaatac atagattgtg
      181 agtaacaagg gataacagat gccctcaaag aacctgagaa acaacgagtt gtaattcaca
      241 ctaacatttg ggactcaaca ttattatccg gcaaatataa gaaggaaatt aagcttggat
      301 attagtaact taactttaaa caaaatgtca ttctctgaaa tgaaagtttg tttcaatagg
      361 tggaatccat gttttgtgga ggaagcaaga tatagaatgt atgaagtgaa ccccatttgg
      421 aggttcaatg tccatgagac acatgagttt gaggtttgca cccaaatttg tgacttaaaa
      481 aagcaacact taacacacaa atttttaaaa ggagaaccac atgtcttaca acatgaataa
      541 ggcacaatca gatgacagtt ccatctataa gatggctctt gattggtcat gcagcattaa
      601 aacaatattc attatttttt tcatcctatg tttaagcaca ctaacatggg cgcaacaaag
      661 ccccaatggc tacacctacc agaacgactg gctatattat tcatactttt ctcaaactaa
      721 taatggcgga tatactcaaa tagaccacat taacatccaa atgaacacca gcgggaatac
      781 tattacgcca gaaacgattc aagtaaatac cgacaatgtt gtgatccaca caaatatcaa
      841 cttgggcata ggtgccggta ggtacatatt aaaattctgg gtaagtaaac agattaacgg
      901 tgaatacgtt aaccaaggat catatgtaag tcagttttcc tatatgcatc agaccaacta
      961 cttcaccgtt ccggcagtgg gaaatggatc attcgttttg tcattcgatt tatacaatct
     1021 gtatggtcaa catgtccaca gtggttatca tgtttttgaa aagactatca gccaacagca
     1081 ggttctgatt aaccacaatc gagatgttct ctttaaaatg cggtcatcga atagtagttt
     1141 gaagcgtcct atgctattgg tcgaaggttt tgatcctcta aacagtcaga gctttgacta
     1201 ttataatgaa aagatttcgc aaatggaaaa cggcaatgcc ggtgtatttg ataatgttga
     1261 cttgtactta ttgaactttc gagatgctac catagactta cgagagaact caaatgtggt
     1321 cattggtgca attaaataca tcaatacact gtaccctcag gagacgctta tggagggtat
     1381 tagcgtttta ggcttctcta tgggtggtgt tatctccaga tatgccttag cttatgcaga
     1441 agaacacgag ataccgcaca attgtacaca attgatcacg tatgatagcc cacatagagg
     1501 agcggttata aacaccaatt tacaagatga gttgtatcat atttacgaca caatagataa
     1561 tttgcaagaa gctgtatcag ccatcgaatg ggcatcgctg attctaccat tgcctggggg
     1621 attgattatt tcaatggctg caagcgattt caataatact ttacttaggt ataaggctat
     1681 gttccagcca atattgaaaa caatcgaagc gccagcgagt agacaactcc tacgttacaa
     1741 tattaactct gacggatata cagatgaatt gaatggatcg atagattatc gagatttcta
     1801 ctcagagatt aatgcagaag acaggcttgc ctattttcca aacactgtta atggagtatt
     1861 gaacaaagat gtaaatgatc caaacaacaa acccggatat ccttacaaac aaaatgcaat
     1921 taaaagcatc gctatatcca atggttggat aaccaaaagt ggcgacatta gtgattcatt
     1981 taataatatt tgcacattta acttgccttc catttgggga aacacccaca ttaatgaaca
     2041 aaattgggac acccaacctg gatcaaccct aggcatggaa tcatgggctg gttcagccaa
     2101 ttcatttcta gatatccact atgatcctgt ttttatccca ctcaaatcca gcctgcatct
     2161 tagacctaca ggatctaatg gctttcctga taatgctgat ttggttatga atatatatga
     2221 tgatatagta ccaagagaca atctaaacaa catcattact gactatcagg aaatcactga
     2281 ctatttatcc agccactcct tgtttgactc tgttgtgctc agcaatgtag ccaatctccg
     2341 ccatgctgat atttctgact tcgttgttac caatgcagta tcaaatttca ggaatccatt
     2401 taatcgggct attggcataa tttcgggcac tattactaat tccgatctaa ctggcattga
     2461 gatgaaagtg tacattggag attcggaatt gcaactaagc caagatttct accgactgaa
     2521 tcctgatggc agttgggctg tagcttacac actgctaaga gactcagatg taaagattag
     2581 attcggcaaa cctggaagcc tgccaaccat aaggaaatac cacattgatt acgataatac
     2641 atcatacagt ataaatggaa ttgataatca acaggtaagc ttgaaggact ttaatctccg
     2701 aaacatcata gtttcccaag caaatacagg cagcttcgaa gagattaacg acgcattacg
     2761 ctttatagtt gagcatttct cagaaaatgt gtataatggg gaagccatca ggatcatagt
     2821 ccttcccgga acatatactg aatcggttga tctttctccc cttgcagctc ttggcatcac
     2881 taacttcaca cttgaaggag tgggcgatgc aataatcgat ggtgatggct atggtatcaa
     2941 tctagtagtt aacgcatatt ccccctgcat tggagccgta tacaacataa gaaagttaaa
     3001 aattataaat agtgatagag gcatactttt caaagattac tgggatgagt catcagggaa
     3061 tgcacaagca ccacagctca ctcttaatat taacaattgc acaatttaca actgcggatc
     3121 aagctcatat tcaggaacaa gtgatcccat attcagtgcc gccgcaatcc actttgaagg
     3181 tgccggaacg atttcttact gcaatatttc taataataac atgctctccg gttcaagtac
     3241 atactcgctt tacatccaag caggaggagt ctatatctcc aacaatacca cttcccttgc
     3301 tgaggtgagg tacagtaagt tcacaagtaa caaaggtggt cttagcggtg gtcttgtggc
     3361 aaagggtaag ggggagattt ggatacaata taatgagttt acaggcaatg agagaagagg
     3421 tccgtgttca gtttataacg cttacaactc aaacgcaatg acggtgtatg atgcttcaaa
     3481 cactctaata aacaacaatc tatttgttga taatatacaa tcgataatcc catatggtgc
     3541 tgtaattggg ctcaatacat acaatactca agcggcagcg ccaatcaggt ttgtaaacaa
     3601 cactattgtg aacacacctc tatataataa caacggtctt tgtgctatag ggtttaggat
     3661 caatgctgga gttgctattc aagatattca tattaaaaac aacattatca gttccactaa
     3721 tagcaatggg tgcacttttg attcgggagc aggatacagc ccggtaagca tcaaatacaa
     3781 tatacttcat aacatcaccc tttccggatt tactgccaat ctctacaatc cagatgatcc
     3841 aaacagcgtt tacaatccta cggctccgag gtttaactac caatgtgatc ctcaactgga
     3901 tgccaattat atccccgcat ggaacgcaac tactatgtct cattgtatag acaccgggac
     3961 cggagtatat gatcctgacg gtacccctcc cgatattgga gctttcagag ctacagatca
     4021 cagatactgg caatattgct ttcgatctgg tcaaaatgat cgtagtgaca cctatcattg
     4081 ggtgagctat ccggttgtaa attcgcttac tcaagggaag acaatcgcaa atgtgttctt
     4141 taaagagctg cttggtaaac atattgagtt aataaacggt gttccacaat ggaagccaga
     4201 tgtcctgcag gaaattcttt ggtatgaggg taatgaatcg agatacattg ttttggaaga
     4261 ttctgattgg ggtccgtacg ttgatacgca taatgttgtt agtcctcaag ggtataaagt
     4321 taagttgcta cctgtagcaa ataatggctt aacccccacc acagttgaat tgcatcatag
     4381 tggtttccta acacctgaaa acaatccttt catgatctat ggtagcagct ccaatggcgg
     4441 ccagacctac gaaaactgga ttggttactt cgatactgat agtgtttggc ctacagatgc
     4501 atttgcctca atctggaacg atatcaccat gatcaaagcc aagaactggt gtctttatag
     4561 agatcccaac agcggaattg gggcattaat tggaaaaatg cttccgataa actgcggaga
     4621 catggttatc gtaactactg tgaacgatca tgaattccaa tggaaaaaga caattcccac
     4681 tgatcctaaa aagaaagagg tcccgaccgc ttttgagtat gttgaaaaag cagattatac
     4741 tcctgtatat attgatttgt ctgctatcga tctatctaac ctgaaagaga tcggcctcaa
     4801 acttgacggg atatgcaaag gggcggttgt tgttactgat tccttggagc agatctgtgc
     4861 ttatctggaa gatggcgaaa gcctgtcttc aggtttggtt gaacttgtgt tcttctacga
     4921 gagtaaaagc cagccgcttg aaatgagaag tgttgctatt aaagacaagc aacttagtag
     4981 cagcatcatt ggtggtgatt ccgcttaccc tgtatacaat atcaaggttt caacagcaga
     5041 cctcagcaat tcggttgttc cggttcttag cctggagcag aactacccca atccctttaa
     5101 tccaaccaca tcaattaggt attcaattga ggagcccgga tcggtaactt tggatatctt
     5161 caacataaag ggtcagttgg tcaagactct gtatcatgga aatgcagaaa ttggcagcca
     5221 tacagtaatc tggaatggct tggataattc cggtaatgca tgttctacag gcgtttactt
     5281 ctataggtta agaaccccca aaacaagtct ggttcgtaaa atgctaatgc taaaatagta
     5341 tcacatcggg gagggcttgt gccctccccc tacctccccc taataggagt agtaatgaaa
     5401 agaaatagat ggattattgc cgggctgtta tgtctgattg gcataggtat gcatgctgta
     5461 accagaattg ttgatatctc cggagcaggg caatatacga gtatccaaac ggctattaat
     5521 gcttcaagct cgggagattc tgtgcttgtg tacccaggaa gatattatga gagcgtcacc
     5581 ataaccacaa atgatatcag tctatttagc cgggaatatc atacgaacaa ccacgattac
     5641 atcaactcta caataattga cggtagtggc aatagtttat gtctgaggat tttgagctat
     5701 actcagaatg tgctggtgcg aggattctct ttgactcatg gaaggggggt cttattatac
     5761 cctaattcct tatcaagatt tgagaattgt aatatttttg gaaatagctc attggatgga
     5821 gcaggaatta atgttagcat ggggtctgta tacttgagtg gagtaaagat atacaacaac
     5881 gatgcctata acatgggtgg cggtatgtat attaatggtt atatgggatc agtatcagtt
     5941 acctttgata cggtgaaccg ctgttccata tacaacaaca cagccggagc agggcaggat
     6001 attgtggctc actccatcaa taatgatttg agtattccgc tggatatgtt cactgttgcc
     6061 aatcccacca gctactatgc tacgtcatta cgagctgctg gagatgagtt tcaattgctg
     6121 ataaatgctc agaggtatca tcatcaggag ataaataatg atctttacgt ttctcccact
     6181 ggagacgaca ccaatgatgg cttgagtccg gcaacagctc tcaagacaat caggacagca
     6241 gtttatagag ttgccacgga cagcctgaac cagaaaactg tgcacatact tcccgggact
     6301 tattccagaa ctgccaacca acaggtattt cctattccat tgaaaagctg ggtaaaggtt
     6361 cagggagcgg ggattgaaga aactcagata gtgggagaga tggatcctgc ctatgccaat
     6421 gtgcaataca atgcactaaa agtctttacc agcttctatc aaactcatac cagcttggag
     6481 gacttggcca tcacttctgc cggatcgaat aacagctgtg caatttgggg ccataaggag
     6541 gaatcactgc atctgaagaa cctaaggatg catgaactta atcccgatga taacgcagta
     6601 atacatatca ggactcccat aaatactgtg tgggataatg ttatcataga gaacattatt
     6661 actgctagac atggtttggt tgcctgccaa gctggaatga ctggtatagt caaaaattcg
     6721 atctttagaa atgcagcgaa cacatttgaa agtctagagg tttggggcta tccactaatc
     6781 tgggtatcag caggagagag tattactgtc gagaattcaa tttttactaa tctatcaatg
     6841 gcagatgatg attcgcaggc tcttagtttc ggtggagtgt cagctccaac attccaacaa
     6901 tattatacgt ttcagaactg tttgttttcg aatataaact gcaacgtaag aggagtgtta
     6961 atagtcggtc atacatatcc agagatgaac atcactaatt gcacttttgc cggacatacc
     7021 ggtaatggtg aggcacttat ggttaatgga aatgtaacaa tctcaaacag catcttctat
     7081 aatgatcgtt gggtggaaat tgccataaat cctatggatg ggacaggtat tcctaccacg
     7141 ctgactctca acaacaacct gatcagaaat ggctatagtg acatctggca aggtccgggt
     7201 agcacgattc actattcgga tactaatatc acgggtaatc cttttttctt gggtggagat
     7261 gatataaata atccgctgta ttacagcctt tctgctgcgt ctccctgtgt taatagtggc
     7321 actccggata ctacgggtct ttgtcttccg ccttatgatt tagcaggtaa ctttcgggta
     7381 tggaacaatc gcatagacat aggctgtttt gagtatgggt ctgagccatg ggtgagtaat
     7441 gacgatccgg tagctccgga tattcctgcg gtcagcataa ccgcctatcc taatcccttc
     7501 agtgtcttta ccaatatcaa agtgagtacc ctaaacgatg gaagtgatag gactgagagc
     7561 gttaataatg tgagcatcgc aatctataac ataaagggtc agagggtaaa aagtattatc
     7621 ttagacccaa tgaaaacaag cgagcagttc acatactggg atgggaggga taaggacaat
     7681 atccgatgtt catctggaat atactttatt aatctgattg taaacggtag aaacatcagc
     7741 actagaaagg taactttcat aagatagacg agtgctttgt ccgtaataca ggtatcaaac
     7801 tcaagtaatg attaatcgac aatctggcaa ttaaaataaa cagttcagtc gttgttctgc
     7861 acttccaatg aaacggtggg aaggaagtaa gtgctccgga gacaccaatc ggttcatcac
     7921 tgccattata gactatctga tcattactga cccatggtgc gagagctttg atgtattctc
     7981 tggcattctc caggttgttg gacttggtgt ccagtgccat tagattatcc atcacttcaa
     8041 aggcatcgtt caggggatag accttgtctt gggcagccag agcccggcag atgtcactgg
     8101 ggcggtcatc caggataacc acgagcttgt agtatctggc tttggctttc ttgtagctct
     8161 gcagccttcc ggactctctg attctgaggg taatatgctc tgtcagtccc tgccagcaat
     8221 gggaagatct gtgccttcag atacgagaca ggcattaccc tgaaaaggat acagtttgat
     8281 gagacccgca ggggaattac ccttcaactc ctcaataagg actttcggct tgagtttata
     8341 gactctgatc agattcagtg gctaacgatg atcgtccctc tggcacttca gttacggttc
     8401 tattaaattt gtaaactatt tcagaaaatc tgatgttctg aaaataccct aaaacgaggg
     8461 caaaattgaa ggaatcgtga tatagatacg tccagaaata tccaaagacc tctgtgacag
     8521 gtgatataga tttgaaggtt tgggtgagaa cttatagtga cggttgattt tcactttttt
     8581 acaccgagag caccgagagc acagagaaca catagagcac cgagatattt tttacaaaga
     8641 gaaaatccgg gttaaaaaaa agcagttgac attgaaagca agattaaaaa aggtagtaaa
     8701 acagataaat gaggggagaa aaaaataagt aatgacgatt gccgaagcat taaaacggct
     8761 ggaaaaaaac atcaaagaga atgagcttat tcataatgga gataagcttt tactgggatg
     8821 ttccggaggt gcggactcca atgctatgct ctatcttttt tcacgactta gagttccgct
     8881 gaatttaact ttattagtta ttcatatcaa tcaccaaatt cggggtgcgg aaagtgatgc
     8941 agatgaggac tttgtgaaac agctttgtca agatttaaat atcccggtaa ttattcggaa
     9001 gatagtagtt cctgaaacgg gtaatctgga aaatcaggca agaaaattgc gctttgaggt
     9061 ttttaataaa attttagaga gttataattt tgataaaatc ctgttggcac atcaaaaaga
     9121 agaccaagca gaaacagtga tgctaaattt tttacgagga gcaggaataa gtggaatggc
     9181 aggaattaaa ggaataacag gaaaagttgt ccatccgcta ttatgtttca gcagaaagga
     9241 attggaagag gttcttaagc aggcgggaat ttcctggcga acggattcca gtaatctgga
     9301 gacaaaattt cggagaaatc ttttacgtaa tgagattatg ccccaattag aaaaggaatt
     9361 caatccagca ttaaaatcgc atctcttttt tttagcggaa cttttttcac aggcagagac
     9421 aatttttaag caaaggagta aaactcaatt ccgacgtata tgtatagaca ttcaaccggg
     9481 aaaagttgtt tttgaactta cccctttatt gaaactaagc ccaattgaaa ggtattattt
     9541 gtttcgcagt gcttttcagt atgtttgcgg aagtgaaaac gatttttttg cggttcactt
     9601 tcaggcaatt gaaaatatat tgaatgggaa aggcagcaaa tgtctgaact tgcctcataa
     9661 aataaccgtt atcaaacaat atgatgaagt tatagtgcaa atagaaacgg aaaaagaagt
     9721 aaaagaaact gaaccattgg ttattgatgc cgaccgcact atggcagttt atggaaatta
     9781 tcgttttaat tttaaatact tacgtgtgtt gcccaaagag caaacagggg atgaattgga
     9841 aagccgggtt attctggatg cggataaaat aaatattcct tttattattc gttttcgcca
     9901 accgggtgac cagtttgttc ccttcggaat gacacagtta aaacggctga aagagttttt
     9961 tattgacgaa aaagtgccta aatatgaaag ggatttaatt cccgtttttg acgacgggga
    10021 aaagttattt tggattgtgg gataccgttt ggataatcgg gttcgctatg atgaaagcac
    10081 aacccgctat ttgcaaataa cggcaacgaa tatttcagta aaatcccgaa aagcaatctt
    10141 gaaaaagaaa agaggaaaca atgaatcaga tgaattgtga tatttccgct gttctttttg
    10201 acgaatacca aattcaaacc cgtattcgcg aactgggctt ggaaatttct aatagctata
    10261 aaaacagcac acctgtgatg ataggtattt taaaaggtgg tttcatcttt ttagctgatt
    10321 tggtgcgcag tataactatc cctgtggaag tggattttct ggctatttcc agttatggaa
    10381 ctaaaaccag cagcagtgga gtggtaaaaa ttcggaagga tatagatatt gacattacag
    10441 gcagggatgt gattgtagtt gaagatattg tggataccgg tctttctttg gcatatatca
    10501 aggattatat ttggcagcat aaaccttcca gcttgaagac ctgtgtgctt ttggacaagc
    10561 cggaagcaca taagacggat gtgacctttg attatatcgg ttattccatt aaaaatgaat
    10621 ttgtggtcgg ttatggactt gactttgcag aaaaataccg taacctaccc tttatcggaa
    10681 ttttgaaaga agagctttat aaatgctaaa atacatactt atttccttat gcctgataac
    10741 attcagtgct cttaatgcta ttcccgcaga taccttaatt actccacagc aaacaattcc
    10801 ggaagcggga aaagcgatta tgcaattttc cacgctgatg aactggttta ttttggcact
    10861 gttgataata tcggtaatca gtatcatcag aatgataatc gtgaaaaaaa gaacccttaa
    10921 cagaaaggat aaacccaatc ccaatcccaa tttacgcaat cctcaaagcc cgaaaaatgc
    10981 tcctcctaag aaatcgcctt tctcgctgcc tttaattata atgttaattc ttttgatagt
    11041 gattatattt catacctaca acagcagtgg aactgccgtt accaaagaga gttacagcaa
    11101 ttttatgacg cgggttgcta atggtcagat aacacaggtg caatttgcgg agaaggacat
    11161 cttttatacc gatatagcga ataagaaata ctctactact atgccttatg aaaatcctca
    11221 actggtggat tcacttgttg ccttgggaat taaggttagc gctacaaaac cctctcgttg
    11281 ggctggagtt atatcttatc ttttgccatt tcttttgctg attatcttct gggtgttttt
    11341 catcagggga atgaatgctc aaaatgccaa agcattcagt tttggaaaaa gcaaagcccg
    11401 cttatacgaa gccagtaaaa cgggaatcac tttcaaagat gtagccggag tagatgaagc
    11461 aaaagaggaa ctgcaggaaa tagtggaatt tttgaaagac ccgaaaaagt ttcaacgctt
    11521 aggtggcaga ataccgcgtg gagttttact tgtagggcaa cctggaacag gcaaaaccct
    11581 gcttgctaaa gctgtttccg gagaagcagg agttcctttc ttttctatca gcggttcgga
    11641 ctttgtggaa atgtttgttg gcgttggagc agccagagtg cgtgatcttt ttgaccaagc
    11701 taagaaaaat gcaccctgta ttacttttat tgatgaaatt gatgcagttg gcaggcatcg
    11761 gggaagcggc ttgggtggtg gacacgatga acgcgaacaa accttaaatc agctcttggt
    11821 agaaatggat ggttttgaac cgaacgaagc ggtaatcatt attgcagcta caaaccgtcc
    11881 cgatattctt gacccggcat tattgcgtcc gggaaggttt gacagaagag tggtagttga
    11941 tttacccgat attaaaggta gaacggaaat tttgaaggtg catacggcta aagtccctct
    12001 ggcaaacgat gtccatctgg aattgatagc cagaggaact cccggtttta gtggtgcaga
    12061 tttagctaat ttggtaaatg aagcagctct gattgcagcc agtaaaaaca aaacccaaat
    12121 ccagatggag gactttgaac aagcgaaaga taagctcctt ttgggaaaag aaaagaagag
    12181 cagagttatt cctgaagaag ataaacggct tactgcttat cacgaaatag gccatgtgct
    12241 tacatccgtg tatctggata aggttgagcc cgtgcataag gtctctatta ttcccagggg
    12301 ttttacaggt ggtgcaacgc attatctgat gaccgataaa accaactatt ccaaaagtta
    12361 tttactgcaa atgctggtaa cacttttagg aggaagagca gccgaagaag ttgtttttca
    12421 agaatatact accggagcag gtaacgattt ggaacgctgt accgatatag ctaaaaaaat
    12481 ggtttgttcc tggggtatga gtgataagat aggtcctata accattggca aggagcaggg
    12541 agaaatattc ttgggtaaag aactcgtttc tcacgatatt ttcagtgaag agacctccca
    12601 actggttgac agagaaatac gggatattat tagtaatgct tattcgcagg caatttctct
    12661 tttgaacgag caccgggaat tgatggatat tatggctaaa gaactgcagg aaaaggaaac
    12721 cttgggcact gatgaaattt ttaatttaat tttggaaaat tgccgtgaag aagaacgaga
    12781 attggtagag gaaaaatata aaaaagccct ggaactacgc tttgaacaca gtgaaaaaac
    12841 aacttcccaa ccaaccgaaa ccacggaaac cgctgaaact acggaaaccg ttgaacaaac
    12901 agaaaacaag gataataaat ctgaggaaac agcataaatg acaatgcacg gtactactat
    12961 aataggtatt caccgcaatg gcaaaacagc aatttgcggt gatggacaag taactatggg
    13021 cgatgctatt gtaaaaggta aagctcagaa aatacgcaaa atttatgagg gaaaggtaat
    13081 tgttggcttt gctggtgcta ctgctgatgc tttcagttta ctggaaagat ttgaagaaaa
    13141 actgaaaacc aataagggta atttgaaaaa agcagctatt gacctggcta aggactggcg
    13201 tcaggataga atcttgagac gcttggaagc aatgcttatt gcgggtgata aagattccat
    13261 tctgcttata aacggagcag gcgatgtttt agagcccgat gataatatta tagccattgg
    13321 cagtggcgga aattatgctt tggcagcagc tcgcgcttta gcacataaaa ccaaactcgg
    13381 agcaagcgaa attgccgtag aagcactgaa tattgcttca ggaatatgca tttatactaa
    13441 taataactat attgtagagg aaatataaat tgttaattaa tgaatattta actcctaaac
    13501 ggattgttga ggaactggat aaatatatta ttggtcaaga ccaggcaaaa cgctgtgtag
    13561 caatcgcttt acgcaatcgg tggcggcgtc agcaaatttc cggtgacatc aagggtgaaa
    13621 ttatgcctaa taatatcatc ctgataggac ctaccggtat tggtaagaca gaaattgcca
    13681 gaagaatttc ccgtttaaca aatgctccct tcatcaaagt ggaggcatcc aaatttaccg
    13741 aagtaggtta tgtaggacgc gatgtagaat caatggtgcg tgaactgatg aactatgcgg
    13801 ttagccaagt gcgcgaagca atgattgaag aggtgcgtcc agaagcggaa aagaatgcta
    13861 cggaaatgat tttagatata ttgcttcctc gtagtaaaaa gatgaagcaa aatgccaata
    13921 acaccgatac acctgaatat gaacgctatt tacgcagtcg ggaaaaaatg cgcaaaaaac
    13981 tgctttccgg tagtttggat gaaaaggaaa ttgaagttga acctgaaacg gataaccttc
    14041 ccagttttga gatttttccg ggaccgggac tggaattact ggattttgac ctcagcgaaa
    14101 tgttttccaa tatttttcaa gggaaaggaa cacccaggaa aatcaaaacc actgtgcata
    14161 ctgcttggaa acgcaatgta gaaaacgaaa ttaaccgtct gataagtaaa gacaaacttg
    14221 ttgaaacagc taagatagca gtggaagaaa acggtattat tttcattgat gaaattgata
    14281 aaatagctaa tactgataaa caggtaggta ttgatgtttc gcgtagtggc gttcaaagag
    14341 acctgcttcc tattgttgaa ggcagtaaag ttcctaccaa atatggaatg attgatactt
    14401 cgcatatctt gtttattgca gcaggcgctt ttaacacggc aaaaccatca gacctcattc
    14461 cggaattgca aggcaggttt cctatccggg tggaattgaa tagtttaacc cagaaggatt
    14521 ttgaacgcat tttaacagaa ccggaaaatt ctttaactaa acagtatcag gcaattttcc
    14581 gaagtgaagg agtagaactg aaatttatgc cggaagcgat tcgggaaatt gcccgttttg
    14641 ctgctttagc taatgaaaaa atggaagata ttggagctcg gcgtttgcat acagtgatga
    14701 ataaactatt ggatgatttt ctgtttgaaa tgccttccga gacactaaaa agcgttaaaa
    14761 tcacgaaaaa aatggtaacc gagcacctta gtcccgtaat tgaaagtgag gacctttccc
    14821 gatttatcct atagcgatgg taatcaataa aataaaagaa atttttgtct ctttaactat
    14881 ccgcaactac cgcatttttt ttatagggca gggaatttct ttaatcggaa cttggataca
    14941 gcgaacaact atgggctggt tcgtttatcg cttaaccggt tctgcttttt tacttggctt
    15001 agtaagtttt ctctcaatga ttccttcggt ttttgtaagt ccctttgtag gtgcttgggc
    15061 agaccgttta aaccgtcatc ggacaatgat tcatacgcaa attgcttttt gcctgcaaac
    15121 aggtattttg gcattattgg ttttaaccag agtaatcaat aagcaggttt ggtatccaat
    15181 tttgatttta tcttttattc aaggaattat tgatgctgtg gatgctccta ttcgtcagaa
    15241 ctttgtaatg gaccttgttt cttccagaac attattgccc aatgctatag caaccaattc
    15301 cgctatgttt aatggagcgc gtttgattgg acctgctatt ggaggttttt taataatgat
    15361 gttcagtgag ggagtttgtt ttgctattaa tgcaatttct tatatacctg ttattatttc
    15421 attgtgtttc ataaagataa gctatcctcc tgttcctcct cagaaagaac cgacgctgaa
    15481 gaaaatattt tcgggatgga agtattcctg ggagaatatg cctattcgct ttttgcttag
    15541 taaccttgct gttttcactg ttttcgggat gtctttttcc actttgatgc caatttttgc
    15601 caaggatatt ttacacggca cttcggcaac gcagggtttg ctaatgtctt caatgggtgt
    15661 aggagcttta tgtggttcat tctttttagc ttcgcgcaaa aatattcggg gaatgccaag
    15721 tcgtttagtt tatttatgtt taactttcag tattgcctgt attttgtttt cgctttccgg
    15781 gtcaattccg cttagtatgt tctttatggt gtttttaggt ttggcaggaa tgatgacaat
    15841 ggctactacg aatactttaa ttcaaagtat ttcttctctg gaaatgcgag gaagagttat
    15901 ttcactttat acaatggctt cagtgagtat gtctccatta ggaagtttgc taatgggaac
    15961 tttatccagc agaattggag ctcgcttcac tttaattgtg tgtgggttgg tattattatt
    16021 gtggtcatta aacggcttga ggatagttcc gaaatttttg cgaggtgtct taagaatgtt
    16081 aattattagc gataatacgg atgtttacag gcaaagagtt ttggcaccgg agatttctgc
    16141 aagatgaaat tattctatta tttggggaaa agagaacctg actaccgcac tttgatgcac
    16201 tttcaaaagg attttatgtt ggaaggaata aaaattcccg tttcgcgttt agttattgct
    16261 gaacaaacac attccaaaga agtgcacatt tgtcgggaaa ttgattgtgg agcaggtatt
    16321 ggtaataagc cccaaattcc tttagcagat ggtttaatca ccaatatacc ctatcaatat
    16381 ttacttattc ggactgccga ttgctatcca attttattaa tggatagtcg cagaaatgta
    16441 gttgcaggtc ttcattgcgg cagagaaggg acacagaaaa atatagttgg agaagcaata
    16501 agaaagatga aaagtcattt ttattgccag ttgaccgata tcatagccat agtaggtgcc
    16561 ggtatttgtc ataaacatta tgaagtaagc caggagctct ttgataactt taatagagct
    16621 ctccgaaaaa tgggattgga accggatata acacaaaaca ggcatcttaa cctcaggaaa
    16681 attctcaatg ctcaacttat taaagaagga attccggaaa tcaatattga aaatgttaat
    16741 gattgcactt ttgaaagttc caattatcat tcctatcgtc gggaaaaaac caataaccgt
    16801 caaataaata ttgtgggtat cctttatggc taaaacttat gttaataaag aaggaaatct
    16861 gattttggaa atccgcgagg aacctctatc tgcttggcta acaatcaaaa aaacggactt
    16921 ccttatagat gaaaacgaaa ttcttgccct tattgaagaa gcaggcatta aaagcggatt
    16981 tgacgaagcg attgattata tatgcaaaca ttcactggaa aaggaatttg aagttccctt
    17041 tcctattgca atgtgcaata aaaaagaagt taccagtatg ttacgctata actttaatcc
    17101 tgatttgctt tcccgtcccg aaaacggaat aaatattagc actttggaga aattgaaggt
    17161 ttttcgttca ggagatgttg tagcagaata ttccagtaat atttttgcac aaggtggcag
    17221 tatctatgat attttcggaa acttacttga tgccaattcg gtagatacgg aacaggcaaa
    17281 agctctagca ggtgataata tagcttataa tgtccaaaac aaacaatttt ccgctcttgt
    17341 agacggcttt ccttatctgg atgaaaatgg ctgtatctgc ctcttggata aggtattatt
    17401 aaacggaaat gaaatcccac cggaaacaaa agttaaatgc cctatcaatt tgattattga
    17461 aggaagcata acttatgccg atattcattg tgaagcagat ataagcgttc agggcgatat
    17521 tcaattctta actattaact gtgctaagaa tatgtttata gcaggggata taatttcctc
    17581 aaacaggaaa ggaattatcg tttggggaaa cctggaatgc cgtagtattc tcaattctta
    17641 tgttctttgc ctgaataaca tccatttcac ggataaaatt gagaattcta ccgttgtttg
    17701 tgacggagaa ataataggtg acttgaacag cagtgagatc tgcggtggct taactcaagc
    17761 aggggaatca attactgttt ttcaagccgg ttcttatgac cggacaaaaa ccgaaataga
    17821 aatagcactt tgcccctttt atcgtaatct gctattgctt ttaaccaggg aacttattca
    17881 cctaaaagaa gataaagaat taaatgctac tacaattgca gaattggaaa accgcattga
    17941 aaaatatgaa agagctttgg acgagaaact aaatcaaatt ctgaaaagcg aaaaacaaaa
    18001 accgaaaaca ataaaagtgc aggatgtaat cagaccacct gttgtatttc gcatcttaaa
    18061 acacagttat gaagttaaca gacaggaaaa aggtcttgaa tttgaggaaa aggactaagg
    18121 aaaatgatta gtgttaacag caaaaaaatc ttggagctat tgtgcatact gatttgcgct
    18181 tgtcctctgg tagcacagga ggcatatttt acgagtgaac ccgccatatc tccagatgga
    18241 gaaatagtgt gttatgtata tagtaatgac ctatggctgg ttccttttac aggaggaatt
    18301 cctcgtagaa taaccaacac aacttcttat gaatggaatc cccaatttag tcctgatggc
    18361 aaaatgcttg ccttttcttc tgaccgctca ggacagaatc tgatttatat aatgccagct
    18421 acgggaggag aggcaaaagc cgtaaattat gagtcattca gaatttcaga atggtttagc
    18481 gattcccaaa atcttttatg taccaaatat tctccgcgtt ggggtttatc tttctataaa
    18541 gttcctattg atggttcgcg tcctattctg cttgctgaaa ttggcagttc ctttgctact
    18601 ttaagtcctg acaacaaaca aatcgtattt cagaaaggtg gctattccag aagagaagca
    18661 tatcggggta gtggcaacgg agaactttgg ctaattgatc ttcctacgaa aaaatatacc
    18721 aagcttacca atacggaact ttcggaattg tatccccgtt tttcgcagac aggagcatta
    18781 tattattgtg cctccgatgg taattgtttt cagatcaaca aggtgaataa actatctttt
    18841 cgcaaaccag aaaaaattac caatctaaaa ccctttagcg cccgcagaat cagtttggca
    18901 agaaataatg accggttggt ttttgaatat tttaatacta tttacaaata cgatcctact
    18961 ctcaaaacgg gagagaaagt aagtccattg ttcatagacc tttgcgaaga cgaatggcag
    19021 gaaaatattg tccgcagtta tgaaaatacc actattgacg attatactat ctctacggat
    19081 gaattattgg ttggttttac ccataattat gatagttttt ttgctccagt taaaggtgga
    19141 gaggcaaaac agattagttt tgaccacggt tcaatattcg gtctgcaatt tttagataaa
    19201 agacagatgg taataagcaa gctggataat ggaattgtga aactttttac ggcaacagca
    19261 gattcggtta tcactctttc tcctgtggat tggtttggag ctgatagttt atgcgttaca
    19321 gagctttata aagataaaaa cggacgctgg tatttcaaat atgatgatta ttacagacac
    19381 caaaaaattg ctattgccga ttcgggtttt gttaatatcc gtccagttaa tacaccttgg
    19441 gctgttacaa ccaattttgc cttcactgaa gacggcagat atgcggtgta tggaacttta
    19501 cgcgaagata attatgtgaa agaattatat ctgtatgatg ttcaggcaga ttccagtaaa
    19561 cgccttttgg ctgataacgg ttggtggcaa aatcttttct ggacttccga taataagtcc
    19621 attctcttaa cccgcaataa tgatatctac cgcttagatt tggttcccag agatgaattt
    19681 gaattggata cagacccttg ggatgaaatc ctgcatccgg aaaagagtaa agccgatagt
    19741 actaatgaag aaaggcaaaa tgaagaaaat ggcattcagg aaattgaaag cacttggaaa
    19801 gagaaaaaag aagaaaaacc caaaaatttg gaaatcgtgt gggaaggatt ggaaaaacgc
    19861 ttttatcctg ttatcactgc aaaagataac tacaatttcg tattggacat agtaagtgac
    19921 agcactttct attatattga gaataacaga cagcaggaaa agaattcaat cctcaaaaaa
    19981 gctaatatct acggaaaaaa tcagaaagaa gtgtttaatt tcggtagaaa tgcaggttat
    20041 tacaataaag ttaataatac agtttattac cttattaatc aagaactgaa gtcctacaat
    20101 ctggatactt cagcccgaaa agatataaaa attaaatacg attacaagta tgataaggca
    20161 cttttaaatc tgcgggtgtt tgaagaggtc tggtgcgcct ttggcaataa cttttatgac
    20221 cctgatatgc ataatgttcc ctggaaagaa ctgtttcaac tctatcgtcc ttatgttgaa
    20281 aaagcaaagg atatctatga tgtggctttc attatagaag aaatgatagg agatgtaaac
    20341 gcttcccata ctggttttta ccccagggaa gaatataaac cctttaccaa tcctgttgcc
    20401 tggctcggag ccgattttga ttataaggat attttagcag aaggaatacg cttggagaga
    20461 atttatccaa cctcccgttt gagcagtttc tatcatcttc aagatggctg tatcttaact
    20521 catattgatg aagttaaaat tactgcacat acttctttag attcattgct ggcaggaaaa
    20581 attgggaaaa agataaaact gactatccta caggaaggaa atattaccga agccgtaatt
    20641 acgggcttaa gctattccca aatgtctgaa ttgaaatatg attatcagat agctaagaac
    20701 aaaaaactgg tggatgaact ctccaacgga aaactgggat atatccatat tcccgctatg
    20761 ggagaaaatg attggcaaaa attctaccgt gattttgtag ttgataattt tgataaagat
    20821 gcactggtaa ttgatgtgcg tggaaactat ggcggacata ttcacgacaa acttattacc
    20881 ctgctccaga aaaagaaata tgcctacaca acaagccgta gatacagtcg tgttatgtgt
    20941 tacgaaccac ataatatttg ggataaacca agcattgtat tagttaatga ggattccttc
    21001 agcgacggag aaattttccc tactgtgtat caggaattga aattgggaaa agttgtcgga
    21061 actccttcca gcggagcagt tattggcacc tggcattact atctccagga tggttcttct
    21121 atgcgaatgc cgggaaccgg atggtataaa ctggatggca caaatatgga aggaacaggt
    21181 gttcagcccg atatagtagt agaaaattta cccgaagata ttattgcaga acgcgaccct
    21241 caactcattc gtgccattaa agaactgatg aaagagctaa agtaaattat gaacctaaat
    21301 gaaataaata atcttattca aagggctagt gccctggtaa aaacaaactg gctgcaagcc
    21361 gtctatatcc ttcaggaagc attgaaagaa gaacccgata atctttccct ccttgttaac
    21421 cttggtgata tttatttgga acgccaactc tatgaaagag ccctcagcta ttatcaaaaa
    21481 gcaatttccc tgcttccaga taatccccag ttgctatttt tgattggtac ttgctatttt
    21541 tccttaggag aatatagaat tgctaatagc tattataatc ggattcctga cccacctccg
    21601 gaaatccttt ataatattgc cttgtcttat gcttttttgg gttcatacca ggaaagtatt
    21661 gatataattc ataaaatttt gaaggtgatg gatgacaatc cttttatata ttttcttttg
    21721 attgaacaat atgtccgcat ccaaaactac gaccgcgctt atgaaatcat tttaactgct
    21781 gaaaataagt ttggtaaaca tagacagttg ttgctccttt ccgcattggt ttacggaaaa
    21841 aagggtatct ggctgaagtc ataccactgc tttgtagaat atgaatcctt aggagaaata
    21901 accaaccccg accatcttat ggcttatgcc aatgcagctg taaatatcgg aatgaacgac
    21961 cgtgctattg aacttttaca aagagctcag gaaataaatc cctatatcaa tgctgtttat
    22021 gaggaactga ttcgcctgta tttgaaaaga aacgattata aaaacgctaa aaaagtcctt
    22081 gacaccgcta aaaggtatat ttcccgtttt tcgcctgtcc tttatctctt caaagaacgg
    22141 ctggataact cgcatttgga atgaaaaatg aatttaaaaa aggttgagag ggttgagagg
    22201 gtttatatgg tttagcttcc taaaggttct tgttttaact cacaattttt aggttgggaa
    22261 accatttgta gtgcagttgg aagtgaagaa gcaagaggga tgctgataat tgggtaaacg
    22321 aatgaaaatt gtgagtcaaa acaagcatcg ttgcaactat attttgtcgc ggtggagttt
    22381 tgactctaaa ccgacccttt tgtgttttca actatattct gaggtaacag gaaattttgt
    22441 tcgttacctt ctactttcaa agctgaagct cggtttagag ttaaaacgaa agtattcccc
    22501 gaagtcacaa attctgaaca attcctaccc cacatttctg gagcggctga atatatattt
    22561 tttccgtgac cgtggaattt gagggaatgc tgtagatcgg ttgcaagtgt gcaggttctt
    22621 gttttaactc acaattttga aatgaagaga ccagttataa tttggttgta agtgtggata
    22681 aattgagggt tttggtttct gtgagggcag tggcaaaatt gtaagtcaaa actttctccc
    22741 cggtgaaaat tgggagtcaa aattttctcc aaccctctca ctgcttcatt tatccgtgtt
    22801 ctcggtgtta aatattattc ctatttctct ttttgcacta tcaatggagt cagaagcatg
    22861 aactgcattt tcggtaattc cttcggcata aagatagcga atagttcccg gttttctttt
    22921 ttcgggattt acatctccga ttaaatctcg caattcctga acagcgttat ctttctggac
    22981 aatgattgca acagtgaaat ccgacatcat aaagttcaac aatttttcaa agaagctctt
    23041 tcctttgtgt tccgcataaa aatgctccgc tagttctcta ttaaaacgga aaattttaag
    23101 cgcgcaaaaa tgaaaattat tttcttccag aatggtgata atatgcccta tatgtttatt
    23161 gcgaactgca ttgggtttaa ttagcagaag tgatttttcc ttcattttgc cgtataaacg
    23221 gtttcaataa aatccagagc ggcaactttg atagcaatat cacggtttag aaccatactc
    23281 attttgattt tgtgctttag gatatcagtc caaatttcat acatctcatt gggcttaaat
    23341 tcaattccgg tgtaaagttt aagcatatcc tggtagaaat catcccggtc agaatcaacc
    23401 ctggcaatta ccagtttagg tgtttcctga caaatatcgt attccttata acggatatcc
    23461 acaactacat agcccaactt tagcaggttt tcatagttaa tctgtaagtc ctcatggttt
    23521 agcatttttt ttaactccca agttaaataa ttctggacaa aacacaggat tctgaatcct
    23581 tgtaaaacaa tgaagaaatc aagccctgct ctgtcaagaa gaatttattg cccgcaaagg
    23641 agcccaaaaa atgcaacgct tcaagcttat aagtgattat actccttcag gtgatcagcc
    23701 cgaagccata gaagccctta ttaaaggtat tgaagaaggc aaaaagcatc aggttctttt
    23761 aggtgttacg ggtagtggga aaacctttac catagctaat gtgattgctc attttaatcg
    23821 tcctaccctt gttttatcgc ataataaaac cctcgcggct caactttatg gtgaattgaa
    23881 acaactcttt ccggaaaatg cggtagaata ttttatcagc tattacgatt actatcagcc
    23941 cgaggcatac attcccggta aggatattta cattgaaaaa gatagcagta tcaatgaaca
    24001 gatagaaaaa ctgcgtttgc gtgctacaat gagtttgatg gaaagaaggg atgtaattat
    24061 cgtttcgtct gtttcctgca tttatggact tggtgttccg gaagaatatc gtgaggcatt
    24121 aatttcccta aaaaaaggta tgaaaatgga ccgtgaagaa cttattcatc aactgatatc
    24181 ggctcattac agcagaaatg atattgcttt tgaaagaggt gctttcaggg ttagaggcga
    24241 tgtagttgat atttttcctg cctatttgga acactgtttg cgcgtggaat tctttgggga
    24301 tgagatttat cggttggaaa aaattcatcc tatttcctgt caacccttgg aagaagtaga
    24361 aaattacgat gtttatcccg caattcactt tttaatgaat gaggaaaaat tgcgcaaagc
    24421 cctcatcagt attgagcagg aaatgaatga acgggttgcc tggtttttac aaaatcaaaa
    24481 atatgtggaa gcagaacgcc tgaaacaacg cacaattttt gacctggaaa tgctccgcga
    24541 actgggttat tgtagtggca ttgaaaatta cacgcgtcac ttaacgggag ctaaacccgg
    24601 agaaccgcca aactgtttac tggattactt tcctgaggat tttttaatgg tgattgatga
    24661 atcccatgta actattcctc aagtgcatgg aatgtttgcc ggggactaca ctcgcaaaaa
    24721 gaacctggtg gaatacggtt ttcgtttacc ttccgctttt gataaccgtc ctttgcgttt
    24781 tgaagaattt gaaaaattta tgcatcaggt aatcttcgtt tctgccactc ctgcggatta
    24841 cgaactaagt ttaactaaag gcgaaattgt agaacaggtg attcgtccca ccggtttgct
    24901 tgacccggaa attgaaatta aacctataaa acatcaggta gatgacctca tcgcgcaaat
    24961 caaagaacgg gttgctaaaa aacaaaagat attggttatg acactaacta aaagaatgag
    25021 tgaagacctt agcatttatc tgaataatgc cggaatcaaa tcccgctata tgcacagtga
    25081 tatagacagc attgaacgag ctaaaatcat cagggacttt cgattaggtg aatttgatgt
    25141 aattgtaggc attaatcttt taagagaagg tttagacctg ccagaggttt cactggtagc
    25201 tattttagat gccgataaaa ccggtttctt gcgttctacc cgttccctga ttcaaatttc
    25261 cggtagagca gccagaaatg ttgatggcaa agtgattttt tatgctgacg aaattaccga
    25321 tgctattaaa caaacagttg ccgaaaccaa tagacgccgt gctaaacagc ttgcctacaa
    25381 tgaagaacac ggaatcactc ctcagaccat ctataaaaca attgaccaga ttatgcaatc
    25441 caccgctatt gctgagggtt atgataaaat tgccaaacaa gctacatttg aaaaaccctc
    25501 cgctaaggaa tttatggaat atctggaact ggataataaa gaaaaaatca ttgaactgct
    25561 cactaaagaa atgaataaag ccgctgctaa ccttgatttt gaacacgctg ctgaactgag
    25621 agataaactt tgggaactgc aacaaaacat ataaaaaatg aagaatgata acacgcagat
    25681 tttattttta caaagagaaa agagagaaag agaataattg agaatgtaaa atgaatttga
    25741 ctgttcttgt ttaaactcac aattttgaaa tgaggagacc aaatgtagtt cggttgacag
    25801 tgtgcttgct tcttgtttta actcacaatt tttaggttgg aaaaccattt atagcacggt
    25861 tggaagtgta gaagcaagag aagtagtgat aattgggaga acaggtgaaa attgtgagtc
    25921 aaaacaagca tcgggttgga gttttgactc taaaccgacc cttttgtgtt tgcaactata
    25981 ttctgactca acaggaaatt ttgttcgttg ccttctactt tcaaagctga agctcggttt
    26041 agagttaaaa caaaagtgcc ccccgaagcc acaaattctg aacaattctt accccacatt
    26101 tctggagcgg ctgaatatat attttttccg tgaccgtgga attcgtgagt tcgttgtagc
    26161 gctgttacaa gtgtgcaggg ttcttgtttt aactcacaat ttttaagttg ggaaaccatt
    26221 tgtagtgcgg ttggaagtgt agaagcaaga gggatggtga taattgggag aacaggtgaa
    26281 aattgtgagt caaaacttcc caggggtgct tcgcaaattt tacgaggggc ttacgccacc
    26341 atcgctatta ttgtgtcgcc ctgacgggct tttatggaat ataaaccctt tcaactttct
    26401 aaaccatctc aaccttctaa acctcaacaa atataaatct accttgtttc ccagtaaaat
    26461 tcattttata ttctacattc tcaattattc tctttttctc attgttctct tcttctcttt
    26521 ttctctttgt aaagaaactc aactttactt tgcacttcat ttccatctcg ctatctctcc
    26581 attataaact catacagcaa atacccattc agcatataca gcacattatc aaattctctg
    26641 tctccaggaa taataatatc cgcatttttc ttagtgggtt caataaatgc ctcatgggag
    26701 ggtttaaccg tgcttaaata ttgttccaaa acgctttcca catctcttcc ccgctctaca
    26761 atatctcttt gcatccgcct ggctaaacgt aaatccgaat cggtatccac ataaatcttg
    26821 aggtcggaaa gagccaaaag ctcaggataa tacagcccga agataccttc cagaataatt
    26881 acatccgcaa aggcaacaca ggtctttcct tcctttctgc aatgagtggc aaaatcataa
    26941 tccggaatat ccactgcctt accttccagc ataagtttca ggtcagaaag cagatagggg
    27001 atgtcaattg cctccggatg gtcaaaattt actttagcac gcattgcagg aggtagatgg
    27061 ctaaggtctt tgtaatagtt atcctgagcc actataacgg tttttaagtc atttaatcgc
    27121 attccgattg ccttggcaat agtgctcttt ccggaacagg ttccgcctcc gataaggatt
    27181 aaacgaacat tattggtttg agacattatt tcctatccag ctgtttattt tagtcaataa
    27241 agcattttct actttttccg gtgtaaaatt agcaaagcag aaagtgctta gtttatttgc
    27301 atattcatcc aatttcttcg cactgcggaa aagttctatt attttttccg ataattcggc
    27361 aaagtcatct gatgcaagat agctatcttc cggtaagggg aagtttttta ttgtggataa
    27421 agtgcaaacc agggttttac gaaattgagc acaggttaaa atccttcctt ccgtatctgg
    27481 tttatttaaa ggcaaaagaa ccagcaatgc ctcctgcaat aattgctctt tttcgctgta
    27541 aggagcataa gttattcttc cgctgcaaag tccttgaagt tcatctcccc cacacagaac
    27601 tatgcttatt tccttttcta ccatcttttt ggatatacgg ggataaatct cggaaataat
    27661 attctttact gcagtaagat tatctttact ttcaggagca ccccaaaaaa ggataaattt
    27721 cttgttgttt cctggtttat caagttctgg tatagtagtt atcggtaaag ggacaaagtg
    27781 tgtattctcc ggcttaaaat gataacattt aaagagctca tcggtttctc caggattagc
    27841 gtataaacaa aatatacctt tcttcggaag atacttgttc caagcagttt gcctaatata
    27901 ttgccaaaga taattagcgt ttttcagttg tttattattt ttccactttt cttttatttc
    27961 cggtaaccaa accttttcaa tatctatgat taatttacat tggtgtaaaa tcttttttgc
    28021 taagcaaact aatggcagac aggcaagtcc tgcaaaaaag attacttcat agcgtttgct
    28081 gtccagaata ttgataaaac gttctttctg accttttcct ataagatgat aagattcaga
    28141 ttcaagatta ttctcatctc tcaagcttaa ggaatataat ttgtcgtaac ccgtatgcaa
    28201 agaaaccggt ttttccatat aaacaggact tttcagcaga agaaggtcag catcaaaact
    28261 tttggaaata acattccaca aaaatcgcga acgctcagct ctgatccctt cttctgcagt
    28321 aaagggctca tcaataaaca atactttttt aataagcata accatatttt ccttcttgcg
    28381 ttcactattt ttgatactgc caactctgtc aagccctgcg cacatctgat tatttttcct
    28441 tcacgccgat ttcgctgatt ccgcagataa aaaagataat aggtctggga tgaacaggat
    28501 taaagggatt tgagagattt ataatataga atgggtcttg ataaaataac aagacacctt
    28561 attattattc catactagtc ctctcttaat tatgcatttt tcattgaacc tctttttctc
    28621 attgtaaaaa aattgcgtaa tctgcgtgct aacattttca ttctcaatta ttctcttttc
    28681 ctctttttct ctttgtaaac caaaatctgc gtgctctcat ttttttggca ttcccatatt
    28741 tttttacagg agccttattt tcatttgatg ctggtcg
//
LOCUS       DAOOZV010000019        27874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1868_length_27874_cov_68.815306, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000019 DAOOZV010000000
VERSION     DAOOZV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27874
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_27874_cov_68.815306"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="PLW08_03310"
     CDS             <1..1931
                     /locus_tag="PLW08_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51664.1"
                     /translation="FDYGTIYYWQVIPHNIIGDAQDCPIWSFTTMDDPSIATFPYQES
                     FDTAVPPNSDWLMRTGALQDPITLSPTSIWEQDDWLNIPGTDKAARINIWGTINGWLI
                     TPIFNIPDDSYYLSFDLAVLKYNQTPTGTPPVYAPDDRFAVLIGDGYSWSTANIVREW
                     NNTGSPYVYNDIRISGEKVVIPLTGHSGHCRFAFYAGSTISNADSDVMINNIEVAAFS
                     NLPLAAQNPIPENNAQDVPLNAKLFWDAGGNAPVSYNLYLGQTLPGTPVECLSPHYTP
                     AALAYGTTYSWKVVPVNPIGEAIDCPVWNFTTTGTATVGAGTVTINDVPINPSVEISG
                     LEGETLISASASFAPEGIGLPNAGLVIQLSSSGVNLTGKHITINHSLGFVPSQIAYRI
                     LPSETYHIISNPGTWTAETVSFILEAKADGDVDIVFPLDAESTLPVELSYFNAIYTAE
                     SYVTINWVSQSETNHSGYNILRSEDRIFANAIRINPALIDKGTELGTQIKYKFIDSEF
                     APNTVYYYWLESISLNGETQYFGPVFVMTGENDSEPETPELPIITKLYNAYPNPFNPN
                     TVISYSVSKPATVTLEIYNSKGQKIRSFINNHNSAGIYSMNWDGKDDTGKTVSSGVYI
                     YRMKTDNFQQSKKMLLTK"
     gene            complement(2040..3020)
                     /locus_tag="PLW08_03315"
     CDS             complement(2040..3020)
                     /locus_tag="PLW08_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HPL51665.1"
                     /translation="MNKLKHHLIPLLIGLIFAIAIWQIAETLIADFWSKTCLSIFGSS
                     FVEMPTMDKNGIPMRYYPSAGKVYDPEFIAYESSRFYKTRIEEPRKNAFLLYSKWLEE
                     HLDENSNIPHNYDYPKAHLKKPWFSSASHSATMLALTERAGNLRSPETFIKARKMLYT
                     LQPEKGNFSFYEKKGGIWFQEFPTEPYSLSGMLKTLVNLSEYYKLVDDSLSWELFQQG
                     VIALQAKLPELAKKNLLDDKYHYKNKRSEHLELVALLEEVNSATPDSVFTPFIQKYQA
                     KSKQFVLFQFFCPFQPGRILGFLIGWAVLYLLAYLFFKPLRTGEHEGERS"
     gene            complement(3241..4026)
                     /locus_tag="PLW08_03320"
     CDS             complement(3241..4026)
                     /locus_tag="PLW08_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPL51666.1"
                     /translation="MFSFSFLVYAFLGALLSGISLAVFAPFVTLRRISYLGEALSHIA
                     FAGIALAILAGLNLTVTTLIFVMLIALGITLLAKKHNIQESNTITIFLSLSMALGIIL
                     ISLSKNYSFDLASYLFGNVLLITRNEIITLAVLSVLNIAFVCFFYKELFYLTYNPVMA
                     KVYRIKTDLVNLIFMLLLAANIVINVKSAGIILVTAQLILPAVIAFNLVHRLSKAIVI
                     SVLVAVFSAFIGFYLSFLLNLPTGACIVVFEAILYFLSLLFSV"
     gene            complement(4033..4761)
                     /locus_tag="PLW08_03325"
     CDS             complement(4033..4761)
                     /locus_tag="PLW08_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51667.1"
                     /translation="MIQIKDLQYKINGKIILEDIFLELAEGEFAAIIGPNGAGKSTLV
                     KLILGLLELKEGSIFIDGVEHYSWLKNNTIGYLPQYEEFDNAFPATALDIVLMGVAGQ
                     LPLGTHFNKNHKQKAMEALEQTGVAHLAKELIGKLSGGEFQRVLLARALVSESKYLIL
                     DEPEASLDRPSVESFFALLKELNSKGKTIITISHDLTTLSKYCSFLVCLNRRLHCHNQ
                     MELIDADVIHKTFGDSLRIIEKDY"
     gene            complement(4770..5678)
                     /locus_tag="PLW08_03330"
     CDS             complement(4770..5678)
                     /locus_tag="PLW08_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPL51668.1"
                     /translation="MKYLKWSMLITLLFAFSCQKQEEKQSPLLLASIHPYELILKQLA
                     GNEFEVKCIIPSNASPHTWSPGPADLKNFSSASFILANGLGLEANLEKNFAERKDVYV
                     EAATLLKDIIPLPERKLLAEEEVPEPVEHQHQHAQNPHLWTSPSLMIRLIGFLEKELS
                     TRFPNSSFVFKHNAQQMQQELKNLIDKIKTERAQYKNPAIITYHNAFHYFLQEFDIEE
                     LGFVQESPGKEPTPKELALLGNIIKEHNVQAIFLEPQMDRKAGETLSKEFNLKLLTLD
                     PLGSDGKAQTIAELIDSNWQKMKAGF"
     gene            complement(5961..7442)
                     /locus_tag="PLW08_03335"
     CDS             complement(5961..7442)
                     /locus_tag="PLW08_03335"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPL51669.1"
                     /translation="MKIDIIRKVPEDMDCIIIFQEENGALDKVELLPQNIAKTAQVYC
                     KSEDFKFAYGDLKNFAIVNGIQRQNIILLGAGNKNDLNTNKVRNLLASAIRFAAKLRA
                     KQIYLFQGFDCPITDVAFGHLLSETALLVTYQFNKYSSSAKNESEIEALHYMLLSKNT
                     RHLNRGIVEGRIYAETTNFARNLVNEPANVITPENLAETAKQAALQYGFSIDVHSLDK
                     LKRIKMDAFLAVGRGSTHEPKLIIMRHLGNPEHHQETIALIGKGITFDSGGYCIKSAQ
                     GMSNMKDDMGGAAAVIGTMCAIATMKLKVNVVGIIAACENMISGDAYHTGDVITSMSG
                     KTIEVVNTDAEGRLTLCDAINYALEKEHCDRIIDIATLTGAAVTALGTQISAVITNNE
                     PWLEQLKNAAHFTGENIWQLPAFEEYKEQLKSEIADLKNSGGPSAGAITAGLFLQEFV
                     QNKPWLHIDIAGTAFKDKEYGIYNYGATGAGVRLLTTLLKYME"
     gene            complement(7474..10494)
                     /locus_tag="PLW08_03340"
     CDS             complement(7474..10494)
                     /locus_tag="PLW08_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPL51670.1"
                     /translation="MNKQLYCLIILFVLCSSVFAYSFGQNKVNFAPQDFSTIQTMHFD
                     IYFPAGEDQFGRIAALMAEDIYYYIKAEFKVPILTRIPIIFYSTKQEFLTTNITYYLL
                     TEGVGGFTESLHNRVVVPFEGSYANLEELLAHELTHAYINAMDNRFVNPNSMLRPTSV
                     PFWFSEGLPEYLSIGGEDDYNNMYLLDMVINNTIGRLNSADGYLAYRLGESFLTFIAE
                     TWGREKVPEYFFTIHSSFNLNEATKKVFGMDFEDLESRWHYQLKRDYYPVINSHDIPM
                     ENFEQRTFHKKDGSYFNLAPRFSPNGSRYVYYSNAGGRYSIWLAGTQGLAQPQLIFKG
                     EKSGKAEEFYYLRSALSWFPDNQRIAFSTKTATGDKIQILNVNTKKIVQTISIPELQA
                     IYEADVSPDGLFLVLAGQKNMQSDLYLYEIETGKLTQLTDDSYDDAHPRFSPEGKYIA
                     YDSEHKCEKQEIRYGLFSDLTRDIFTFNIETGEIVERTFENYDCFSPIWADDGSKLVF
                     LSYKHNLANYEILDWETGQRAELTKCIAGIMNGDISFDDSYIVISNYFDGAWDIYFDD
                     NPLKNLIYEESNQPQSFVRADDLLQTIDLTRLDYYGKRTKAQHLSKKQTPQQNPRRPH
                     ISNNESDKSDSLKHFQDFSWDDKPDSINVPPKIEKYKTKFALDSIWGGLAYSSYTGTV
                     GAIDLTLSDLMGNYGIGISLGISGKIEDSNLFLSFMNLKHRADYGIGVYNFYDETLYR
                     HYRIGQDDYFRLRERELGMLFIYRYPFSRFTRLEFDSQLYYVKQEWDYLAPEDVPTEN
                     WQMDISKETDTVIAPGLAFVFDNALFGSTGPMVGWRYYYLIRKSFAKNELNYFTNYLD
                     LRCYNFFEKRYSLAFRLNAGISTGESPQRFNLDGLYGVRALNEDIDGERMILGSAELR
                     FPFFDYLYLAFPVPLQLRNIRGSIFADMGAAWDKNSDFRGVIDGKLNDIKLGYGFGPR
                     LNLGYFVLKFDIAWLTDLSKISKPMYYLSLSEDF"
     gene            complement(10503..>10828)
                     /locus_tag="PLW08_03345"
     CDS             complement(10503..>10828)
                     /locus_tag="PLW08_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51671.1"
                     /translation="VITVNKIEDLTSSLQEVIAENKEPLKNTLVEGNITIEKLSATLD
                     SLKILSANLNRSAKTLTESEGTAGLLLNDKQLYEKITTATDNLNALIKDIKENPKKYI
                     KFSVF"
     assembly_gap    10829..10928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10929..11560)
                     /locus_tag="PLW08_03350"
     CDS             complement(<10929..11560)
                     /locus_tag="PLW08_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPL51672.1"
                     /translation="MKKFYPNLRSVQIKTGIWTIVICLILVFSYFWLSGRLAVRSHYE
                     LKIAFPNVMGLEVGDKVMYRGMEVGRVKSITASKDKVITTANIRSDIILTEGTRFLVS
                     DSSLMGGKALNIIPGDGKNPLNIKKLQQGTSPEGMMELISKASAGLDELNETVKLLNS
                     PDGLMQSSRKLVQNTDDTVTEMGNIAKEIKQELVITVNKIEDLTSSLQEV"
     gene            complement(11567..12862)
                     /locus_tag="PLW08_03355"
     CDS             complement(11567..12862)
                     /locus_tag="PLW08_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPL51673.1"
                     /translation="MFRKALVCVGGKRISVILLCLIVFSSFTIIPLSADILIPMDNTQ
                     TNHLKAYGIAFAGLKEHLVGRWLLNYRGGSFLFPDSESLTALCNIRGVRYENVSSSDV
                     AAILMEIQANNMESIALEKEPKIAVYIPPNALPWDDAVTLALDYAEIDYDRVWDDEVL
                     AGKLPEYDWLHLHHEDFTGQYGKFYGSYRNTSWYQNDVRVNEAMAAKHNFAKVWQLKH
                     AVAEKIRNYVINGGFMFAMCAATDTYDIALAAKDVDIVDAAIDGDGIDPQYQSKLDFS
                     RTFAFENFTLRPNPYEYEFSDIDASDYSSLRGPEADYFQLFDFSAKYDPVPTMLTQCH
                     TNVINGFLGQTTSFFKDKVKKSVIILAEVPGQNEVKYIHGNIGKGTFTFYGGHDPEDY
                     QHKVGDPETILELHKNSPGYRLILNNVLFPAAEKKQLKT"
     gene            complement(13082..13984)
                     /locus_tag="PLW08_03360"
     CDS             complement(13082..13984)
                     /locus_tag="PLW08_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPL51674.1"
                     /translation="MNPFIEKIIPLILAFFVGIAFKLLKLLSKDDAPVLLRVVLNVCL
                     PSLTIIAISNIQLKADLALIPFTAIIIVFLMYIISRTIGKHLKMEKTTFGSFLVGTMI
                     MNTAYALPFFFAAFGNEGLARASLFDIGNTFMIFTFTYYNAIKYGNNSKTAAIEWKKF
                     LRLPPLWAMLIAFSWKLTKVPMPALALNFLNFMGQPMVPLMMIALGLYFNPKRCNIGK
                     AAIAIFIRMGIGLGIGIVLSALFGFKGITRTVVTVCSALPIGFNTLIFANLENLDREF
                     AATIVSISIFIALFYIPWLIYVFQ"
     gene            complement(13981..15462)
                     /locus_tag="PLW08_03365"
     CDS             complement(13981..15462)
                     /locus_tag="PLW08_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPL51675.1"
                     /translation="MIFIYLLSGLFLGWSLGANDTGNIFGAAVETRMLKFKTAALIAS
                     IFIFLGAVFEGSGPSGTLGRLGSVDALGGAFTVALAAALTITAIVRTGIPVSTSQTIV
                     GAIIGWNFFSGRLTDFRNLLTIVSSWVIAFVLSGIIAALLFYLIRPVINRGKIHLLEQ
                     DVYIRLGLIIIGAFGAFSLGANNIANVVGVFVPVTPFKDINLGSFTLSGVQQLYILGA
                     ISIIVGIYTYSHKVMRTVGKDIFHLSPVTALIAVAAEAIVLFIFASRGLQNLLLSIGL
                     PPIPLVPVSSTQIIVGAVVGIGLVKGGKNIRYNILGKVSLAWIAAPVIAFFFAFIALF
                     IVQNVFEQTVYQKTIYTFNHTTIRQIEKEGLDVNHLSSVNGRKFYREMVVYKELKAEK
                     YFSRSEILKIIRITEVYPLKVNTKLLQDKGLAIRFTEQQWEALVKLEGREFRHKWQLQ
                     ETLAKDPSWQIRKEITERDKLHNQDLEEQFNLLFRTFYLPTEQ"
     gene            complement(15463..16107)
                     /locus_tag="PLW08_03370"
     CDS             complement(15463..16107)
                     /locus_tag="PLW08_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPL51676.1"
                     /translation="MALILKTTRFIETQIDAFLDILSDSATTFELAMEDYLNNRIEQF
                     ETRLAQIRENEHRADDLRVSIERFLYERTLIPENRGDVLAILENTDEVIDNIKDSLVQ
                     FSIEMPKIPECLNDLWMQTTKASSNAVEQLSVAVRSFFRDLAAVNNYIHKVYFFEREA
                     DHIGERLRREIFNLDIELSQKSQLRFFAIHIEKISDYAQVVCDRLSIYAIKRQL"
     gene            16788..17828
                     /locus_tag="PLW08_03375"
     CDS             16788..17828
                     /locus_tag="PLW08_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPL51677.1"
                     /translation="MKADITNNTFSEIILYPVEEGKYEIALNKDSESIWLNLNQIAEL
                     YQTSKANISMHIHNILKDGELTRKATVKNYLTVQIEKGRQVHRNIRYYNLDMILAIGY
                     RVHSERGTQFRIWATQHLSEFLHKGFILDDERLKGNDRLTDYFDELLARIRDIRASEK
                     RAYLRVREIFALAIDYKPNSKAANKFFATMQNKMHYAATGMTAAEIIATRANAQVTNM
                     GLTNWKGNIVRKEDVSIAKNYLTSDEIDILNRIVNMFLEQAEFRTLRKQQIYTKDWEK
                     FLDKFLMDNELPILNGSGKITHNEAKELAENAYNEFELRRRKAIEDDAEAKYLADLTK
                     ATKLVSARRKKR"
     gene            complement(18050..19213)
                     /locus_tag="PLW08_03380"
     CDS             complement(18050..19213)
                     /locus_tag="PLW08_03380"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPL51678.1"
                     /translation="MITALSMWKPDKNDTFAFPRFQLIILIEGERIMPYFRSDLKDKK
                     PSKISVPVKKRMMCLNESCLNPYQAIKHQFLAKMEEIELNRYLSPVTEKLHLSLISYI
                     GNSLTKENVLWGNGADDILYHIFLAVRENDKSFAVSLAPSYFDYKTFSAMVGLKMLFL
                     DLDEDFSFSPEKYLELASHPDCKLAILCNPNNPTGNLFPDCQLLQIIKALPDKLVLVD
                     ETYFEFSGRTLADKLWQYSNLILVRSFSKAFSSAGLRFGYAISSAESIYELSKVLTTF
                     HTSILNQAFALTILENQDIFKAQVQQTIQLREELFATLQTIEEIKVYPSATNFLTFSL
                     AERTPEFFNHLLANDIAVRDVGSHPRLQNCLRVTISCREDVEAFVNALKLFLR"
     gene            complement(19367..19936)
                     /locus_tag="PLW08_03385"
     CDS             complement(19367..19936)
                     /locus_tag="PLW08_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPL51679.1"
                     /translation="MKKIIFLTLALIFVCGALLAQTDAPKDRSNPKVQSEGIKSPKPE
                     MPMQKCLEELKLTDAQKKKFEELKISFEKNKNTLNAEIKNLKIDLQKALKDENYKQAK
                     DLNKQIATKRNVLADAKVDFLAARMKELTPEQKATLKKNMPHFQGAMHKQTPCKNAKE
                     FRPQSRENCDECGECGSFGMPEKKSPPKQ"
     gene            complement(19933..20385)
                     /locus_tag="PLW08_03390"
     CDS             complement(19933..20385)
                     /locus_tag="PLW08_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPL51680.1"
                     /translation="MSKRWLIIILLISVSFNLAFIGSFIYLHWFHPHPQPPVRNEEMR
                     GSRPHFGPPPFERDEEIRNLRDKFETIKHSLMLELAKDPVDMAKVNALIDSSLVAQNN
                     LERRLAERMVAYRKTLTAEEAQEHFQRRAEFAKKRLNRDNIPKNRRNR"
     gene            complement(20378..20824)
                     /locus_tag="PLW08_03395"
     CDS             complement(20378..20824)
                     /locus_tag="PLW08_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPL51681.1"
                     /translation="MRCSKAKRYLEKQLDGELDIRFQAKLQSHLDKCPACKVFLADAQ
                     KLHLMLSHLPKPEFPSWIHGQIMDKVHRLEAKNPGFFRRYKLAPVTAMLTIVLSLWAG
                     VKVGIVSYNNIIPEKTESITLTSVAYGSFGENTILDNIAENGEQGE"
     gene            complement(20870..21358)
                     /locus_tag="PLW08_03400"
     CDS             complement(20870..21358)
                     /locus_tag="PLW08_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPL51682.1"
                     /translation="MKKEHFEAFLRANEKHIYNYVLTLCANEQDALDIVQTVFIAVYK
                     NLDRIDEATALAYTYKIARNKCLSFLKQKAHYINMEPERFNCIPEKTATANETDYTVL
                     KNAISALPPRLAAVIQLQYYEKMSYKEISATLGISIKAVESLLVRAKRTLRKKILQDK
                     ES"
     gene            21694..22950
                     /locus_tag="PLW08_03405"
     CDS             21694..22950
                     /locus_tag="PLW08_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPL51683.1"
                     /translation="MINKSVVFLARKYLSGRKTRGIGKAHYLCLIGITLGVLSLLCVT
                     SVMNGFRLDIRNRITGTFAEIRISAKGGETIKGYSAICNQLQDMKFSASPVIRNELLL
                     KYDSVVLPTLCFGIEPNRHKNVSSLLQKPDTGSKEIIQGLLVGNISPEEFSASGIALG
                     AGLASQLGVYLGDEIQVLSPMFNVPTAFGMIPRIRFLKVQAIFAAGMPEYDQNFSFMG
                     IDNAVFFASYSPGEADYIEVKPFSQMRKNQPLKTLKKAFPDFQIEDWSSFDPNLYSAI
                     RFEKFLMFVIMLFMFIIASFNLTGNLLKMISQKKRELGLLKALGLTDKDLQSLFLVQA
                     LFLCSAGIILGLTLGSLLLLIQKYTGIVKLGLGGGNSIILPVKFAFTDYLLITVVAYL
                     ITILSVLLPLKRLNKINAVELIKRAV"
     gene            complement(23419..23598)
                     /locus_tag="PLW08_03410"
     CDS             complement(23419..23598)
                     /locus_tag="PLW08_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51684.1"
                     /translation="MKNKRTELNLKEEKVLLTFIQQTKEGKYVKIDESSFPYSRIKEQ
                     SEDTYFEDEYYYSIR"
     gene            complement(23673..24164)
                     /locus_tag="PLW08_03415"
     CDS             complement(23673..24164)
                     /locus_tag="PLW08_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51685.1"
                     /translation="MTETGSLGTIDAQMKLERAKKIAAELTNHSKWRSHGRSIKIKDL
                     EGIGIKITRVDDDAKLAENIYRIQTVCRLLFESTSTFKIFATQDNKIFKQAVPVGAPR
                     ILPGKPTINAVEIEQKCPKCGKKYNLYAKLVPNPKLILILKIKDLYPSPRSVNLFVSV
                     VLK"
     gene            complement(24244..25368)
                     /locus_tag="PLW08_03420"
     CDS             complement(24244..25368)
                     /locus_tag="PLW08_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51686.1"
                     /translation="MKLNGKTIYAHSSDIKSRTYLEYRKDMKKKAIAELEILEWLEDK
                     IKELYPEKCVKVYKSGGDKFLWFLRKGGISREPDYIAEINDKKIEFEFQYAEKADLEF
                     YDFKVSKIAKSKKGNREPIKDKFIIYIYKPSYQYAIFKAEWVFNNSQYGMVEAWRTYA
                     YRVPKDKFLSILKPDTNLKSICKRIDAKNFILNFQHQLLDINKDKLSNLLQGVIDEDK
                     IVKLMPKDLDSFFKVCFILDHLNKIPQNANLWLIYLLTYINKDILLEDITKIVYCIDF
                     LYSKIDVKPNELNQLINKVEELILKIKSFYQDDGSYKSSLTSSPLEETRYALFSINLL
                     EDLIQDIIYYYSVSELQPIKKIYENVNDIEKTSKLIREAK"
     gene            complement(25381..26433)
                     /locus_tag="PLW08_03425"
     CDS             complement(25381..26433)
                     /locus_tag="PLW08_03425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51687.1"
                     /translation="MEPTDYKNRTYLSAIEASKFLGITVEMLHNIVNQQLIKAQIAAS
                     GQMRFDLKELKDFSANLKYKSHSNNIDIDKVNTLEINGTIQKIYIKNSMKMDDLEDNS
                     IHLMITSPPYFDTKMYSGEPLPGDLGNIHNIDEWFEKIGEVWKEVYRVLQPGRKAFIN
                     IMNLPVRLEKGKFRTLNLAGRTIDLCEKIGFIFKRDIIWQKTNAVRAHFGTYPYPGGI
                     LINNMHEFILEFDKPEKKGFNKYGHLTKEQKEQSKLDKDFWLSIKKSDVWVMKPQGSG
                     DNRNHIAPFPYELPFRFIKAFSYVDETILDPFVGSGVTLSAAADLKRNGIGYEIYPEI
                     AYEAVKALRYHQTEIW"
     gene            complement(26827..27507)
                     /locus_tag="PLW08_03430"
     CDS             complement(26827..27507)
                     /locus_tag="PLW08_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51688.1"
                     /translation="MPRIQSTGKCHFCFRTFSKSAMTKHLASCKAMQPAWESSLEYDE
                     TQSVKCFHLLVEGRYLPEYWMHLLVTANISLKRLDTFLRDIWLECCGHLSAFKIEDKT
                     YSVMPMPELKEKSMRFKLNEVLYPGIKFYHKYDFGTTTFLTLKVISEMELKAKGRFII
                     IMARNDPPEKKCYVCGKPATQVCVACIWDSKGWLCDKCASKHKCGVDMLLPVVNSPRV
                     GVCAYTGE"
BASE COUNT         8736 a         5251 c         4942 g         8845 t
ORIGIN      
        1 gatttgatta tgggactatc tattactggc aagtgatacc tcataatatt attggcgatg
       61 cacaagattg tccaatttgg agttttacca caatggatga tccttccatt gctacttttc
      121 cttatcagga gagttttgat acggcagttc cccctaatag tgattggttg atgagaactg
      181 gtgctttgca agacccaatt actttaagtc ctacaagtat atgggaacag gatgactggc
      241 tgaatattcc tggaaccgat aaggcagcca gaatcaatat atggggtaca ataaacggtt
      301 ggttgattac tcccatattt aatataccgg atgatagcta ttatctttct tttgatttgg
      361 cagtgctaaa atataatcaa accccaaccg gaacacctcc agtttatgct ccggatgata
      421 gatttgctgt tttaattgga gatggatata gttggtctac agccaatatt gtaagggaat
      481 ggaataatac gggttctcct tatgtttaca atgatatcag aatcagcgga gagaaagtag
      541 ttattccctt aaccgggcat agtggccact gtcgttttgc cttttatgcc ggttccacaa
      601 tttctaatgc cgatagtgat gttatgatta acaatataga agttgccgct ttcagtaatc
      661 ttcccttagc tgctcaaaat cccattcctg aaaataatgc tcaagatgta cctcttaatg
      721 ctaaactgtt ttgggatgct ggaggcaatg cacctgtttc ctataactta tatttgggac
      781 aaactcttcc cggaacaccc gtagaatgtt tatcgccaca ttacactcct gcagctttag
      841 cttatggaac aacatattcc tggaaggttg ttcctgtaaa tccaataggg gaagcaattg
      901 attgtcctgt ttggaatttt actaccactg gaacagccac ggttggtgcc ggtacagtta
      961 ccatcaatga tgttccgata aatcccagcg tagaaatttc cggcttagaa ggtgaaacat
     1021 taataagtgc cagtgccagt tttgctccgg aaggtattgg tttacctaat gctggtttag
     1081 taattcagtt aagcagttcg ggagttaatc ttacaggaaa acatataacg attaatcatt
     1141 cgcttggctt tgtcccttcc cagattgctt atcgtatttt acccagtgaa acatatcata
     1201 ttatttccaa tccgggaacc tggactgctg aaacggttag ctttattttg gaagctaaag
     1261 cggatggtga tgtggatatt gtttttccgt tggatgcaga atccacttta ccagtggagc
     1321 tatcatattt taatgccata tacacagcgg aaagttatgt taccataaac tgggtaagcc
     1381 aatcggaaac aaatcatagt ggttataata ttttgcgtag cgaagataga atatttgcca
     1441 atgctattcg gataaatcca gctttgatag ataaaggaac cgaacttgga actcagatta
     1501 aatataaatt tattgattct gaatttgcgc ctaatacggt ttattattac tggcttgaaa
     1561 gtatttcctt gaatggtgaa acgcaatatt ttggccctgt ttttgttatg acgggagaaa
     1621 atgattctga gccagaaact cctgaattgc caattataac aaaattgtat aatgcctatc
     1681 ctaatccttt taatcctaat acggttataa gttacagcgt atctaaacca gcaactgtaa
     1741 ctttggaaat atataattcc aaaggacaga aaatccgttc gttcataaac aaccataatt
     1801 cagcgggaat ttacagtatg aattgggatg gcaaagatga tacaggtaaa acagttagta
     1861 gtggtgtata tatctatcgt atgaaaaccg ataatttcca acaaagcaaa aaaatgctac
     1921 tgacaaaata attcgctgaa agcggaagtt agtgtaagaa tcccgggtga aaatgcccgg
     1981 gattttttta actattgaga acaccgaaaa cttttaaact gcagacagac ctcttgactt
     2041 caagacctct cgccttcgtg ctctccagtc ctcaatggct taaaaaataa gtaagcaagc
     2101 aaatagagaa ctgcccaacc aataaggaaa ccgagaattc tgcctggttg aaaaggacaa
     2161 aaaaactgga acaggacaaa ctgtttgctt tttgcctgat acttttgtat aaaaggcgtg
     2221 aaaacagaat ccggagtagc agaatttact tcttccaaga gtgcaacaag ttctaggtgt
     2281 tcactgcgtt tgtttttata gtgatattta tcatccagta agtttttctt tgccagttcg
     2341 ggcagttttg cctggagagc aataacacct tgttgaaaaa gttcccagga taagctgtcg
     2401 tccaccaatt tatagtattc ggataaattt acgagtgttt tcaacattcc ggaaagggaa
     2461 tagggttcgg taggaaattc ctgaaaccaa atgccacctt ttttttcata aaaactgaaa
     2521 ttgccttttt ccggttgcag ggtatagagc attttcctgg ctttaataaa tgtttcgggg
     2581 ctacgcaggt tgcctgctct ttctgttaaa gcaagcattg ttgcactatg actggcggaa
     2641 ctgaaccagg gtttttttaa atgtgctttt gggtaatcat aattatgggg aatattacta
     2701 ttttcatcca gatgctcttc cagccattta ctatacagca aaaaggcatt tttccgcggt
     2761 tcctcaatgc gggttttata aaagcgggag gactcataag caatgaattc aggatcataa
     2821 acctttccgg cagagggata ataacgcata ggaatgccat ttttgtccat tgtaggcatt
     2881 tccacaaaag aactgccaaa aatacttaaa caggtcttac tccaaaaatc ggcaataagg
     2941 gtctctgcta tttgccaaat tgctatggca aaaataagac cgatgagcaa aggtatcagg
     3001 tgatgtttca atttattcat atttaccttc ttttaagtta agaatcattt acacttcaag
     3061 tgaggagtca ttggcgtctt tataactttt gtccttgttg ataacctttg gacgcaaagg
     3121 actcatcacg acgataacaa aacctctcat aggttacact ttctaacacg gttaaaatag
     3181 gtaagggact aacagtgtct tccggctaca caaaacttgc ggaggaacag catcctcctt
     3241 ctacacagag aaaagcaaag agagaaaata aaggattgct tcaaatacaa ctatacaagc
     3301 accagtaggt aaattcagca gaaaactcaa ataaaatcct ataaaagcgg aaaaaacagc
     3361 taccagaaca gagataacta ttgctttgga aagccgatgc acaagattaa aagcgattac
     3421 tgcaggcaaa ataagttgag cagttaccag aataattcct gcgcttttca cattgataac
     3481 aatattagca gctaaaagca gcataaaaat caggttcacc aggtctgttt taatccgata
     3541 gaccttagcc atcacaggat tataggttaa ataaaagagt tccttataaa aaaagcagac
     3601 aaaggcaata ttcaaaacgg ataaaacagc cagagtaatt atttcgtttc tggtaatcag
     3661 cagaacattg ccaaaaaggt agctggcaag gtcaaaactg taatttttag aaagagaaat
     3721 taggattata cccagtgcca tacttaagga taaaaatatg gttattgtat tggattcctg
     3781 aatgttatgc tttttggcga gcaatgttat tcccaaagca atcagcatca caaaaatcag
     3841 ggtagtaaca gttaaattca atcctgccaa tatagccagg gcaattccgg caaaagcaat
     3901 atgggaaagt gcttcaccca aataggatat ccgccttaag gtgacgaaag gagcaaaaac
     3961 agctaaagaa attcccgaaa gcaaagcacc caaaaaagcg taaaccagaa acgaaaagct
     4021 aaacattttt gcctaataat ccttttcaat aatccgcaaa ctatcaccaa aggttttatg
     4081 tatcacatcc gcatctataa gttccatctg attatgacaa tgcaaacggc gattcaagca
     4141 aaccagaaaa ctgcaatatt tagagagagt agtcaaatcg tgagaaatag ttataatcgt
     4201 tttgcctttg ctattgagtt cttttagcag ggcaaaaaaa ctttctacgg aaggacggtc
     4261 taaacttgct tccggttcat caaggataag atatttgctt tccgatacca gggcacgcgc
     4321 taaaaggact ctttgaaatt cacctccgga aagttttcca atcagttctt ttgccagatg
     4381 agcaactcct gtttgttcca atgcctccat tgctttttgt ttatggtttt tattaaaatg
     4441 cgttcccaaa ggaagttgac ctgccacacc cattaaaaca atatccaaag cggttgcagg
     4501 aaaggcatta tcaaattcct catattgggg tagatagcca atggtattat ttttcagcca
     4561 gctatagtgt tccacaccat ctataaaaat actgccttct ttcaattcca ataaaccgag
     4621 aatcagcttt accaaagttg atttacctgc tccgttagga cctataatag cggcaaattc
     4681 accttcggca agttccagaa aaatatcttc cagaattatc ttgccgttta ttttgtattg
     4741 gaggtcttta atttgaatca ttagtattgc taaaatcccg ctttcatctt ttgccagttg
     4801 ctatctatca attcagcaat ggtttgtgct ttgccgtcac tacctaaagg atcaagggta
     4861 agcagtttca agttaaattc tttagagagt gtttcaccag cttttctatc catttgcggc
     4921 tccagaaaaa tagcttggac attatgttct tttatgatgt tgcccaggag agcaagttct
     4981 tttggagtag gttctttacc aggactttcc tgcacaaagc ccaattcctc aatatcaaat
     5041 tcctggagga aatagtggaa agcattatga taggtaataa ttgccgggtt tttgtattga
     5101 gctctttccg ttttaatttt atcaattaga ttttttagtt cctgttgcat ttgttgagca
     5161 ttgtgtttga agacaaaaga agaattggga aagcgagtgc taagttcttt ttccagaaaa
     5221 ccaatgaggc gaatcattaa cgagggagag gtccataaat gggggttttg agcgtgttga
     5281 tgttggtgtt caactggttc gggaacttct tcttccgcaa gtaatttcct ttccggcaga
     5341 ggaataatat ctttcagcaa agtagcagct tccacataaa catcttttct ttccgcaaaa
     5401 ttcttttcca gatttgcctc caatcctaaa ccattggcaa gaatgaaaga agcggaacta
     5461 aagtttttta aatcggcagg accgggtgac caggtatgag gtgaagcatt tgaaggaata
     5521 atacatttaa cctcaaattc atttcccgca agttgtttca gaataagctc gtagggatga
     5581 attgaagcaa gtagcaaagg actttgcttt tcttcctgtt tttggcagga aaaagcgaag
     5641 agcaaagtga taagcatgct ccatttaaga tacttcattt ttacctccta agctttttta
     5701 caccaagaac accgagttta ccgaaagcac cgagtttatt ttataattat tagcaatgct
     5761 aattttcatt ctcaattcag catataattt ttccggctaa ctataaacca ttttcccaat
     5821 tccatctttc catcttacag tctgctattt tcagagcatc tcactatatt ttagatttta
     5881 cagttcacac tttcttattt cgcatcatcc acccatcgtt aaggatgggt tgatgatagg
     5941 tggcaagcaa ggaagaaatc ctattccata tattttaaaa gagttgtgag caaacggaca
     6001 cctgctcccg ttgcaccgta gttataaatt ccgtattctt tatctttgaa ggcggttccg
     6061 gcaatgtcaa tatgcagcca gggcttattc tgcacaaatt cttgcaggaa aagacccgca
     6121 gtaatagcgc cggcagaggg acctccggaa tttttcaaat cggctatttc ggatttgagt
     6181 tgttctttat attcttcaaa agcgggaagt tgccagatat tttcaccggt aaagtgagct
     6241 gcatttttca gctgttccag ccagggttcg ttattggtaa tcacggcaga aatttgtgtt
     6301 cccaaagcgg taactgctgc acccgttaaa gttgcgatat caataatgcg gtcgcaatgt
     6361 tctttttcca gagcataatt gatagcatcg caaagagtta agcgaccttc agcatcggta
     6421 ttgactactt caatagtttt tccgctcatt gaggtaatta catctccggt atgataagca
     6481 tcccctgaaa tcatattttc gcaggcagcg atgatgccta ccacattaac tttcaatttc
     6541 atagtggcaa ttgcacacat agttccgata acggcagctg cacctcccat atcatctttc
     6601 atatttgaca ttccttgtgc acttttaata cagtaaccgc cgctgtcaaa ggtgatacct
     6661 tttccgatta aagcaatggt ttcctgatgg tgttcaggat ttcctaaatg gcgcataata
     6721 ataagttttg gctcgtgagt tgagccgcgt cccacagcta aaaaggcatc cattttgatg
     6781 cgtttaagtt tatccaggga atgcacatca atagaaaaac cgtattgcaa agctgcctgt
     6841 ttggctgttt ccgcaaggtt ttcgggagta ataacattgg caggttcatt taccaaattc
     6901 ctggcaaaat tggttgtttc ggcataaatg cgtccttcta ctatacctct gtttagatgg
     6961 cgggtatttt tagagaggag catataatgc aatgcctcaa tttcgctttc gtttttagcc
     7021 gagcttgagt atttattaaa ttgataggta accagtaaag ccgtttcgga aagcaaatga
     7081 ccaaaagcca catcggttat agggcaatca aacccttgaa aaagatagat ttgtttggct
     7141 cttaatttgg cagcgaaacg aattgcggaa gccaaaagat tgcggacttt attagtattg
     7201 aggtcgtttt tattgccggc acccaataaa ataatatttt gtcgttggat tccattcacg
     7261 atggcaaagt tctttaaatc cccgtaagcg aacttaaaat cctcactttt acaatagacc
     7321 tgagccgttt tggcgatgtt ttgcggtagc aattctacct tgtccaatgc cccgttttct
     7381 tcctggaaaa tgattataca atccatatct tccgggactt tacggatgat gtcgatcttc
     7441 attctgaact ccttattgtt tttttatagt atattaaaaa tcttcggata agcttaagta
     7501 atacatcggt ttggaaattt tagagaggtc agtaagccag gcaatatcaa attttaacac
     7561 aaaataacct aaattgagac gaggtccgaa accgtaaccc aattttatat cattcagttt
     7621 accgtctatc actcccctaa aatcagaatt tttatcccac gcagctccca tatcggcaaa
     7681 aatgcttccg cgaatattgc gtagctgtaa aggtacggga aaagccaaat agagataatc
     7741 aaagaaagga aaacgcagtt cagcagaacc caaaatcatt ctttctccgt ctatatcttc
     7801 atttaatgcc cgcacaccat acaaaccatc aagattaaaa cgctgaggtg attcacctgt
     7861 actgattcct gcattcaaac gaaaagccag agaatagcgt ttttcaaaaa agttgtaaca
     7921 gcgcaggtca agatagttgg taaaatagtt cagctcattt ttcgcaaagc ttttccgaat
     7981 gagataataa taccgccaac caaccatagg tcctgtagag ccgaaaagag cattatcaaa
     8041 aacaaaagcc aagccaggtg caataaccgt atcggtctct ttggagatat ccatttgcca
     8101 gttttctgta ggaacatcct ccggtgccag ataatcccat tcctgtttca cataatacag
     8161 ctggctgtca aattccaaac gcgtaaaccg actgaaggga taacggtaaa taaaaagcat
     8221 tcccagttcg cgttctcgca agcgaaaata atcatcctga ccgatacgat aatgcctata
     8281 aagcgtttca tcatagaaat tatagacccc tattccgtaa tcagcacgat gctttagatt
     8341 cataaaagaa aggaaaagat tgctgtcttc aatttttcct gaaatcccca aactgatacc
     8401 tattccataa ttgcccatta aatcacttaa agtgagatca atagctccca cggtgcccgt
     8461 gtaagatgaa tacgccaatc cgccccagat gctgtctaaa gcgaattttg ttttatattt
     8521 ttctattttg ggcggaacat taatactatc cggtttatca tcccaggaaa aatcctgaaa
     8581 atgtttcaag gaatcgcttt tgtccgattc attattggaa atatgaggac gacggggatt
     8641 ttgttgtggg gtttgttttt ttgaaagatg ttgtgcttta gttctttttc cgtaataatc
     8701 aagacgggta aggtctatgg tttgtaaaag gtcatcggca cgcacaaaac tttgcggttg
     8761 gttgctttct tcataaatta gatttttcag gggattatcg tcaaaataga tatcccaggc
     8821 accgtcaaag tagttgctta taactatata actatcatca aagctgatat cgccattcat
     8881 aataccggca atgcatttag ttagttcagc gcgctgtccg gtttcccaat ccagaatttc
     8941 ataattggca aggttatgtt tataggaaag aaaaacgagc ttggaaccat catctgccca
     9001 aattggacta aaacaatcgt aattttcaaa ggttctttca actatttcac cggtctcaat
     9061 attgaaagtg aaaatatcgc gcgtaaggtc tgaaaacaaa ccataacgga tttcctgctt
     9121 ttcacactta tgttcactat cgtaagcaat gtatttgccc tcgggtgaaa aacggggatg
     9181 agcatcgtca taactgtcat cagttagctg agttaatttt cctgtttcaa tttcataaag
     9241 atagaggtcg gattgcatat ttttttgacc tgccaaaacc agaaacaagc catcgggaga
     9301 aacatccgct tcataaattg cttgcaattc aggaatgcta atggtctgaa ctattttttt
     9361 agtatttaca ttcaggattt gtattttatc accggtagct gtcttggtag aaaaagcaat
     9421 tctttgatta tcgggaaacc aggaaagagc cgaacgcaaa tagtaaaatt cttctgcctt
     9481 gccacttttc tcacccttaa aaatcaattg cggttgagct aatccctgag ttccagccag
     9541 ccaaatactg tatcttccac ctgcattaga ataatataca tagcgtgaac cattgggaga
     9601 aaaacgaggt gccaaattga aataggaacc atctttttta tgaaatgtcc gctgttcaaa
     9661 attctccatc ggaatatcgt gactgtttat cacaggatag taatctctct ttaactgata
     9721 atgccagcgg gattccaggt cttcaaaatc cattccgaaa acttttttcg tagcttcatt
     9781 aagattgaac gaggaatgaa tggtaaaaaa atactcggga actttttctc tgccccaggt
     9841 ttcagctata aaagttaaaa aactctcgcc taaacgatat gccaagtagc catcggcact
     9901 gttcaatctg ccgatggtat tatttatcac catatccaac aaatacatat tattatagtc
     9961 atcttcgcca ccaatggaaa gatattccgg aagtccttcc gaaaaccaaa aaggaacaga
    10021 agtgggacgg agcatagaat tgggatttac aaaacgatta tccattgcat ttatataagc
    10081 atgggtaagt tcatgtgcca aaagttcttc cagattggca taactgcctt caaaaggaac
    10141 cacaacacgg ttatgtaaac tttccgtaaa accgcctact ccttcggtta aaagataata
    10201 agttatattg gtggttaaaa attcttgctt agtgctgtaa aatataatag gaatccgggt
    10261 taaaattgga actttgaatt ccgctttgat gtaataataa atatcttcag ccattaaagc
    10321 cgcaatgcgg ccaaattgat cttcccctgc gggaaaatag atatcaaaat gcattgtttg
    10381 aatggtagag aaatcctgag gagcaaaatt aactttattt tgaccgaaac tgtaagcaaa
    10441 aacggaactg cataagacaa agaggattat cagacaatat agctgtttat tcattagctt
    10501 tcctaaaata cgctgaattt gatatatttt ttagggtttt ccttaatatc tttaatgaga
    10561 gcattgagat tatcggttgc agtggtgatt ttttcatata actgcttatc atttagtaat
    10621 aaaccggcag taccttcgct ttcggtaagg gttttggcag aacgattcaa attggcagaa
    10681 agaattttta acgaatccaa agtagcagaa agtttctcta tagttatgtt tccttccacc
    10741 aaagtatttt ttaaaggttc tttattttcc gctataactt cctgcagaga agaggttaaa
    10801 tcctcaattt tatttactgt aataacgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnat aacttcctgc agagaagagg ttaaatcctc aattttattt actgtaataa
    10981 cgagttcctg ttttatttct tttgctatat tacccatttc agttactgtg tcatcggtat
    11041 tttgcactaa ctttcgggaa ctttgcatta agccatcggg agaattaagt aatttaaccg
    11101 tttcattcaa ttcatccaag cccgcagatg ctttactaat caattccatc ataccttccg
    11161 gggaagttcc ctgttgcaat ttttttatat ttaggggatt ttttccatct cccggaataa
    11221 tgtttaatgc ctttccaccc atcaaagaac tgtccgaaac cagaaaccgt gttccttccg
    11281 ttaaaattat atcacttctg atattggcag ttgtaattac cttatctttg ctcgcagtta
    11341 tgctttttac tctaccaact tccattccgc gatacataac tttatcgcca acttccaaac
    11401 ccatcacatt gggaaaggca attttcagtt cataatggga acggactgct aaacgaccgc
    11461 taagccaaaa ataactaaaa accagaatca gacagataac aattgtccag atacctgttt
    11521 ttatttgcac gctacgcagg ttaggataga atttcttcat taccttttag gtcttcagct
    11581 gtttcttttc agcagcgggg aagaggacat tattcaggat taaacgatag ccgggtgaat
    11641 ttttatgcag ttctaatatg gtttccggat cgccaacttt atgctgataa tcctctggat
    11701 catgaccacc gtaaaaagtg aaagtccctt tgccaatatt gccatgaatg tatttaactt
    11761 cattctgacc gggaacttcc gctaaaataa tcacactttt ctttacctta tccttgaaaa
    11821 aagaagtggt ctgacccaga aaaccgttta taacattagt atgacattgc gtaagcattg
    11881 tgggaacagg atcatacttg gcgctgaaat caaaaagctg aaaataatcc gcttcgggac
    11941 ctcttaaact ggaataatcg ctggcatcta tatcggaaaa ttcatattca taaggattgg
    12001 gacgcaaagt aaaattttca aaggcaaaag tgcgggaaaa atccagttta ctttgatact
    12061 gagggtctat gccatcaccg tcaatagcgg catctacaat atctacatct ttggctgcta
    12121 aggcaatatc ataagtatcg gtagcagcac acatagcaaa cataaaaccc ccgtttatta
    12181 catagttgcg tatcttttcc gctactgcat gcttcaattg ccaaaccttg gcaaaattat
    12241 gtttagcagc cattgcttca tttacgcgca catcattctg ataccagctg gtatttctat
    12301 aagaaccata aaacttgcca tactgacctg taaaatcctc atggtgtaaa tgcagccaat
    12361 catattccgg aagcttgcct gctaaaactt catcatccca aacacggtca taatcaattt
    12421 ccgcataatc caaagccaaa gttactgcat catcccaggg aagagcatta ggaggtatat
    12481 aaaccgctat tttaggttct ttttccaggg caatgctttc catattattt gcctgaattt
    12541 ccattaaaat tgctgctaca tcggaagaag aaacattttc ataacgcaca ccccgaatgt
    12601 tacaaagagc agttaaactt tcggaatcgg gaaaaagaaa acttcctccc cgatagttta
    12661 aaagccatct gccaacaaga tgttctttca gacccgcaaa ggcaatgcca tatgccttta
    12721 gatggttggt ctgcgtgtta tccataggga ttaaaatatc ggcagataaa ggtattatag
    12781 taaaactgct aaacactatt aagcatagca gaataactga tatccttttt cctccaacac
    12841 acaccaacgc ttttctaaac aaggatttat cctccttaat ttaattcctg cttatataaa
    12901 gctattttta tgccataaaa cgcataaatg tcttatgctg tcaagataaa tctcataaac
    12961 aacgaaaaca aagaggatac cgaagaaaaa gagtattttt tacacggaaa acaccgagag
    13021 caccgaaaaa aattatacaa ctaataaact tccaatatag ctccagccct ctctacccct
    13081 ttcactgaaa cacataaatc agccacggaa tataaaacag agcaataaaa atggatatag
    13141 aaacaatagt agccgcaaac tctctatcca aattttccaa attggcaaaa ataagagtat
    13201 taaaaccaat aggcaaagca gaacaaacag ttactacagt tctggttatt cccttaaacc
    13261 caaaaagagc agatagaaca atacctatgc ccaaaccaat gcccattctg ataaaaatgg
    13321 caattgccgc ttttccgata ttacagcgtt tgggattaaa ataaagtcct aaggcaatca
    13381 tcattaaagg gaccatcggt tgccccataa aattgagaaa attcaaagct aaagctggca
    13441 tcgggacttt agttaatttc caggaaaagg caattaacat agcccaaagg ggaggtaaac
    13501 gcaaaaactt tttccactca attgccgctg ttttgctatt atttccatat tttatagcat
    13561 tgtaataggt aaaggtaaaa atcataaagg tattgccaat atcaaaaagt gatgctctgg
    13621 caagtccttc attgccaaaa gcagcaaaga aaaaaggcaa ggcataagca gtattcataa
    13681 tcattgttcc caccaggaaa ctgccgaaag tggttttttc cattttcaag tgttttccta
    13741 tagtgcggga gataatatac atcagaaaga cgataataat ggctgtaaaa ggaattaaag
    13801 ccaaatccgc ttttagctga atattggaaa tggcaataat ggttaaagaa ggtaaacata
    13861 cattaagcac aaccctcaaa agaacaggcg catcatcttt agacagaagc tttagaagtt
    13921 tgaaggcaat gcctacaaaa aaagccagaa taagaggaat tatcttttca ataaagggat
    13981 tcattgctca gtaggaagat aaaaagtgcg aaatagtagg ttaaattgtt cttccaggtc
    14041 ttgattatgt agcttatctc tttcggtaat ttcttttctt atttgccagg aagggtcttt
    14101 tgccagtgtt tcttgcaatt gccatttatg cctgaattct cttccctcaa gtttaactaa
    14161 tgcctcccat tgttgttccg tgaaacgaat tgccaaacct ttatcctgta gaagcttggt
    14221 atttactttc aaaggataaa cctcagtgat acggataatt ttcagtattt cagaacgcga
    14281 aaaatatttt tctgctttta gttctttata aaccaccatc tcgcggtaaa atttacgacc
    14341 attcacagaa gacagatgat taacatccaa gccctctttt tctatctgac ggatagttgt
    14401 gtgattgaaa gtatatattg ttttttggta aacggtctgt tcaaacacat tctgcacaat
    14461 aaaaagagct ataaacgcaa agaaaaaggc aataactggt gcagctatcc aagctaaaga
    14521 taccttgcct aatatattgt aacggatatt tttaccccct tttaccaaac caattccaac
    14581 aaccgctccc acgataattt gggtggaaga tacaggaact aaaggaatgg gaggtaaacc
    14641 tatacttaat aataggtttt gcaaaccacg cgatgcaaaa ataaaaagga caattgcttc
    14701 tgctgctaca gctataagtg ctgttaccgg tgaaagatga aaaatatctt ttcctacagt
    14761 tcgcatcact ttatgcgaat aggtgtaaat tccgacaatt atagaaattg cacccaaaat
    14821 atagagttgc tggacacccg aaagagtaaa actgcccaaa ttaatatctt tgaaaggagt
    14881 taccggaaca aaaactccaa ccacattagc tatgttatta gcacccaaag aaaaagcacc
    14941 aaaggcaccg attataatta accccaaacg aatataaaca tcttgctcta aaagatgaat
    15001 ttttcctcta ttgataacag gtctgattaa ataaaaaagc aaagcggcaa taattccgga
    15061 aagcacaaaa gcaattaccc agctggaaac aatggtaagt aaattacgaa agtcagttag
    15121 ccgtcccgaa aaaaagttcc aaccaataat tgcacctaca atggtttgag aagtggaaac
    15181 cggaattccc gttcggacaa tagcagtaat agttaaagca gctgctaggg caactgtaaa
    15241 agcacctcct aaagcatcaa cacttcctaa acgacctaag gttccggaag gaccgctacc
    15301 ttcaaaaaca gctcctaaaa agataaaaat ggacgcaatt aaagccgctg ttttgaattt
    15361 tagcattctg gtttctacag cagcaccaaa aatattgccg gtatcattgg ctcctaaaga
    15421 ccaacctaaa aaaagaccgc tgagcaggta aataaaaatc atttatagtt gacgcttgat
    15481 agcataaata gataaccgat cacaaacaac ctgcgcgtaa tccgaaattt tttcaatatg
    15541 tatggcaaaa aaacgcagct gacttttttg ggaaagttca atatccaaat tgaatatttc
    15601 acgacgcagg cgttcaccta tatgatccgc ttcgcgttca aagaaataga ccttgtgaat
    15661 gtaattgttt accgcagcta aatccctaaa aaatgaacgc actgcaacag agagctgttc
    15721 aacggcattg cttgatgcct tagttgtttg catccacaaa tcatttaagc actctggaat
    15781 ttttggcatt tcaatggaaa attgaaccag agagtccttt atattatcaa taacctcatc
    15841 tgtattttct aatatagcaa gaacatcacc tctgttttcc ggaattagag ttcgttcata
    15901 aagaaagcgt tcaatgctaa cccgtaaatc atctgcccga tgttcatttt ccctgatttg
    15961 agccaaacgg gtttcaaatt gttcaatccg gttattgagg taatcttcca ttgctagttc
    16021 aaaagtggta gccgaatcac ttaaaatatc caaaaaggca tctatctggg tttcaataaa
    16081 gcgggttgtt tttaaaatca gagccatttt ctctccttga gagttataat tttattcctt
    16141 ttattatttt accagaaaaa gaaatactac tttctgtcaa gcgaaaaggg aactcaggtt
    16201 aacgggtgaa caggttaata aatgaaaccc caagataaat taagggcaac ctgaaatatc
    16261 tcttttagat tgaaaaagct ctttgccatt ctcgcggaag caggaattca taaaagtttt
    16321 aacaaaataa ctacattttc aatttggaaa ctatgtaatt tctattgcac atactttgta
    16381 acgggtaata tggataaata attggatgct caataaaaaa attatgttag aaaaaataga
    16441 ggttactttt gtgtggacag ggtatgattt taaggataaa atgaaatatc ccttgacaat
    16501 agaagttact tgagatagca gaactatgga agttatatga ggtaatttta tatgattcaa
    16561 gctattagct ataaacccct gaatttagag ggagaaaaat ataaaaattc ttaattaatt
    16621 gagcttttga atactgtatt gtttaggaaa atataggatt atttggttag taaagttaaa
    16681 aggacattac cgataattta caaccatttc aattttatag cgttattcaa aagtacataa
    16741 tatatatttc aaggtcttta aaaaaataaa taaattcaag aggaattatg aaagcggaca
    16801 taacaaacaa cacttttagt gaaattattc tttatcctgt agaggaagga aaatatgaaa
    16861 tagctctaaa taaagatagt gaatctattt ggttgaatct caatcaaata gctgaacttt
    16921 atcaaacatc taaagctaat atcagtatgc atatacataa catcttaaaa gatggtgaat
    16981 taaccaggaa agcaactgtt aagaattact taacagttca aattgaaaag ggacgacaag
    17041 ttcatagaaa tataaggtat tacaacttag atatgattct agctataggt taccgggtcc
    17101 attcagagag aggaactcaa tttcggattt gggctactca acatttaagt gaatttttgc
    17161 ataaaggttt tatacttgat gatgaaagat tgaaaggtaa cgatcgtctt acggattact
    17221 ttgatgaatt gttagctcgc atcagagata ttagagctag tgaaaaaaga gcatatttac
    17281 gggtaagaga gatttttgct ctggcaattg attataaacc taatagtaaa gctgctaata
    17341 agttctttgc tactatgcaa aataaaatgc attatgctgc aaccggtatg acagctgcgg
    17401 aaattattgc cacaagagct aatgctcaag ttaccaatat gggacttact aattggaaag
    17461 gaaatatagt aagaaaggaa gatgtgtcta ttgcgaaaaa ctatcttact tcagatgaaa
    17521 ttgatatttt gaatcgtatt gtaaatatgt ttctggaaca ggcagagttt agaactttaa
    17581 gaaaacaaca aatttatact aaggactggg agaagttttt agacaaattt ctgatggata
    17641 atgaacttcc aattttaaat ggatcgggca aaataactca taatgaagct aaagaattag
    17701 ctgagaatgc ttataatgaa tttgaattaa gacgcagaaa agctattgaa gatgatgctg
    17761 aagcaaaata tttagcagat cttacaaaag caactaaatt agtttctgca agaaggaaaa
    17821 aaagataact atgggttaac gggtgaacag gttaacaggt gaaaagttta acagatgaaa
    17881 ccccaagata aattaagggc aacctgtaat atgtttgcct ggtgcaaata caactatatt
    17941 tttcacttac ccggactcct gcttccgctg gaaggataag gtaatttttt atgctaaaaa
    18001 tgatacaact tcttctcgta gtataaattg acctctctac tacaagagct tagcgcaaga
    18061 acagtttcaa cgcattaaca aatgcctcaa catcttccct gcagctaatt gttaccctta
    18121 aacaattttg caaacgggga tgagaaccta catcgcgaac agcaatatcg tttgctaaaa
    18181 gatgattaaa gaattcagga gtccgttctg caagagaaaa agtgaggaaa ttggtagcag
    18241 aaggataaac ctttatctct tctatggttt gcaaagttgc aaaaagctct tcccgcaatt
    18301 gaattgtttg ttgtacctga gctttaaaga tatcctgatt ttccagaata gtgagagcaa
    18361 atgcctggtt cagaatagaa gtatgaaagg tagtaagcac tttggaaagt tcataaatgc
    18421 tttccgctga agaaattgca tagccaaaac gcaatccagc agaggaaaat gctttggaaa
    18481 aagaacgcac taaaatcaaa ttggaatatt gccataattt gtctgccagt gtccttccgg
    18541 aaaattcaaa ataggtctca tcaaccagca caagtttatc aggcaaagct ttaattattt
    18601 gtaatagttg gcaatcggga aacaaattgc ccgtaggatt attgggattg cataatatag
    18661 ctaacttaca atccgggtga ctggcaagtt ccaaatactt ttccggggag aaactgaaat
    18721 cctcatccaa atccaaaaat agcattttca gtcctaccat agcgctaaag gttttataat
    18781 caaaatagga aggtgccagg gaaacagcaa aggatttatc attttcccgc actgctagaa
    18841 agatatgata gagaatatca tcagctccat ttccccacag gacattttct tttgttaagg
    18901 aattgccaat gtaagaaatt agggataaat gcagtttttc cgttacagga gagagataac
    18961 ggttaagttc aatctcttcc attttggcta aaaattgatg tttaattgcc tgatatggat
    19021 ttaggcaact ttcgttcagg cacatcattc tttttttaac gggaacactt attttagagg
    19081 gctttttgtc tttcaagtca ctgcgaaaat agggcataat tctttctcct tctatcaata
    19141 ttataagttg aaatcgggga aaagcaaaag tgtcattctt atccggtttc cacattgaaa
    19201 gtgcagttat cattgaaaaa atatccttga ttcttgcttg tgcagaaatg acaagagcat
    19261 ttcattccag caatgatatt attttaaaaa aagccacggg aggagtaccc gtggctttgt
    19321 ctgtgtgttg ctgaggaggg attattaggg gcttattttt cttgctctat tgtttgggag
    19381 gtgatttttt ttcaggcata ccgaaagaac cgcattctcc gcattcatca caattttctc
    19441 tgctctgagg tctgaattcc ttagcatttt tacagggtgt ttgtttatgc atagcaccct
    19501 gaaaatgtgg catatttttt ttcagggttg ccttctgttc gggggtaagt tctttcattc
    19561 tggcagctaa aaaatccact ttagcatcgg ctaagacatt gcgtttagtg gctatttgtt
    19621 tgtttaaatc ctttgcctgc ttgtagtttt catctttcaa agctttctgc aggtcaattt
    19681 ttaaattttt aatttcggca tttagggtgt ttttattttt ctcaaaactt attttcagtt
    19741 cttcaaattt tttcttttgg gcatcggtaa gttttaattc ctcaaggcat ttttgcatcg
    19801 gcatttcggg tttcggggat ttaattcctt ccgattgaac cttaggatta gagcgatctt
    19861 taggggcatc cgtttgggca agtaatgcac cgcaaacgaa tattagggca agggttagga
    19921 atataatttt tttcatctgt ttctcctatt tttaggtata ttatctctgt ttaagcgttt
    19981 tttggcaaat tcagcacggc gttggaaatg ttcctgtgct tcttctgccg ttaaggtctt
    20041 acgataggca accattcttt ccgccaatct tctttccaga ttgttttgag ctaccaggga
    20101 agaatcaatt agggcattca cttttgccat atctacaggg tcttttgcta attccagcat
    20161 cagggaatgt ttgatagttt caaatttatc ccttaaattc ctgatttctt catccctttc
    20221 aaaaggagga ggaccaaaat ggggacggga acctcgcatt tcttcgtttc ttacaggtgg
    20281 ttgaggatga gggtgaaacc agtgtaaata aatgaaactg ccgataaagg caagattaaa
    20341 actcaccgaa atcagcagga ttattattag ccagcgttta ctcaccctgt tctccatttt
    20401 ccgctatatt atccagaatt gtgttttcac caaaactgcc gtaagcgacg gaagtgagag
    20461 taatggattc cgttttttcc ggaattatgt tgttatagct aactataccg actttaacac
    20521 ctgcccataa gcttaagaca atagtgagca tagcggtaac tggggcaagt ttatagcgac
    20581 ggaaaaatcc cggatttttt gcttccagac ggtgcacctt atccataatt tgtccgtgaa
    20641 tccaggaagg aaactctggt tttgggaggt gagaaagcat cagatgcagt ttttgggcat
    20701 ctgccagaaa aactttgcag gcaggacatt tatccagatg ggattgaagt tttgcctgaa
    20761 agcggatatc caattcgcca tcaagttgtt tttccagata gcgtttggct ttactgcatc
    20821 tcatttttct gttccttctt atattttttg cttccaatta ctataccatt caactttctt
    20881 tatcctgcaa aatttttttc cttaatgtgc gttttgctct taccagcaag gattcaaccg
    20941 cttttatgct gattcccaaa gtagcagaaa tttccttgta ggacattttt tcgtaatact
    21001 gtaattgaat tacagctgcc aaacgcggag gcaaagctga gattgcattt tttagaactg
    21061 tgtagtccgt ttcgttggcg gtagccgttt tttcgggaat acaattaaat ctctccggtt
    21121 ccatatttat ataatgtgcc ttctgtttta gaaagctgag gcatttatta cgggcaattt
    21181 tataggtata agcaagagct gtggcttcat ctatgcgatc cagatttttg tataccgcta
    21241 taaatacggt ttggacgata tccaaagcat cctgttcatt agcgcataaa gttaacacat
    21301 aattatagat gtgcttttcg ttagccctta aaaatgcctc aaaatgttct ttcttcatac
    21361 ctgtttttac ttatcatagt attttttgaa tgtaaattcc tttatcattc ctgtgcaacc
    21421 aggaatccac atacgggaaa aaaatattta tgcattttaa tatggaattc acatcatatc
    21481 ttaagtgtgt tttttttaac ttgcaataaa caaaccaaac ccgaaacaaa gctttaaggt
    21541 gtagccggag aacactgatt ctccgcattt tttgtaaccg taacttatcc aaatttcgta
    21601 ttattttcac gcagaaaagt ggcttcctcc gtctacactt ccaaaacaaa acttgactaa
    21661 tttccctctt atcagttagc agtaacaact aagatgatta ataaaagtgt agtttttctt
    21721 gcccgcaaat acctgagcgg acgcaaaacc agaggcatcg gtaaagctca ttatctttgc
    21781 ctgattggca taactttagg agttttgtct ttgctttgtg taacttccgt gatgaatggt
    21841 ttccgcttgg atattcgtaa ccggattaca ggtacatttg cagaaattcg tatttcggca
    21901 aaaggagggg aaaccatcaa aggttactct gctatttgta accaattgca agatatgaag
    21961 ttctctgcca gtcccgtaat tcggaatgaa ttgttgctga aatatgattc tgtagttttg
    22021 cctacgcttt gttttggtat tgaaccaaat aggcataaaa atgtctcttc ccttttgcaa
    22081 aaaccggata ccggcagcaa agaaatcatt cagggtttgc ttgtgggaaa tatttctccg
    22141 gaagaatttt ccgcctcagg catcgcttta ggggcagggc tggcttcaca attgggtgtt
    22201 tatttgggag atgaaattca agtgctatca ccaatgttca atgttccaac tgcatttgga
    22261 atgattccgc gcatccgttt tctgaaagtg caggcaattt ttgctgcggg aatgccggaa
    22321 tatgaccaaa atttcagctt tatgggcatt gataacgctg tcttttttgc ttcttattcc
    22381 cctggcgagg cagattatat tgaggttaaa cccttttccc aaatgcgcaa aaatcagcca
    22441 ctgaaaaccc tgaaaaaggc atttccggac tttcaaattg aggattggag cagttttgat
    22501 cccaatcttt attctgccat ccgctttgag aaattcctga tgtttgtgat tatgctcttt
    22561 atgtttatta ttgccagctt taacctgacc ggcaatctgc tgaaaatgat ttcccaaaag
    22621 aaacgcgaat tgggcttgct aaaagcttta ggacttactg ataaagacct acagagctta
    22681 tttttagtgc aggcactttt tttatgttcc gcgggaatta ttctgggctt aactttgggc
    22741 tcacttttac tgctcataca aaaatatacg ggaattgtaa aactgggttt gggaggagga
    22801 aattccataa ttttacccgt gaaatttgct tttacagatt accttttaat tactgttgtt
    22861 gcctatttaa tcactatttt aagcgtgcta ttgcctctca aacgcctaaa caaaataaac
    22921 gctgtagaac taattaaacg cgcagtttag aaacttcttt ctcgcgcata tattgctcct
    22981 cagattccct tatgcttccc atatcgcgat atgggaagca taagggaatg atatgggaag
    23041 cgtaaaattt accataaaga aagccataaa tgttattgat gtatcttttt gttctaaaaa
    23101 acttatgaag ctaaaatgta agtgacagaa gaaaattata attcaaataa cccataacga
    23161 attgtgaatt tcaaacaatg ccagtgaatt attgtgtagc agcattgatc cattgggttg
    23221 caaaattcat cttttgcact gcaaccaaat gtatcccaag aaatttatgc tttatatatt
    23281 taaccgagat gaattttcaa acctatatat tattggtatt atattggaca atcccttcct
    23341 ttcccgcaaa aacaggaata caaacaaaat ttacccaaaa tacctgcata tccaatttaa
    23401 aaatggtata atattatgct atctaatgct ataatagtat tcatcttcaa aatatgtatc
    23461 ttcactttgt tctttaatcc ttgagtaagg aaaagagctt tcatctattt ttacatattt
    23521 tccttccttt gtttgctgta taaatgttag caataccttc tcttctttta ggtttaattc
    23581 tgttctctta tttttcatga ttatatctcc ttattaaact attatttttt tacctgtttg
    23641 tatttctatc tggtttttga tacctaataa atctatttca aaaccacact cacaaataaa
    23701 tttactgacc ttggggaagg gtataaatcc tttattttta aaatcagtat caatttaggg
    23761 ttaggaacaa gtttggcgta aagattatat tttttcccgc atttgggaca tttctgttca
    23821 atttcaaccg cattaattgt tggctttcct ggtaaaattc ttggagctcc tacaggaact
    23881 gcctgcttaa atattttgtt gtcttgtgta gcaaatattt taaaagttga agtgctttca
    23941 aaaagaagtc ggcaaactgt ttggatccta taaatatttt cagctaattt tgcatcatca
    24001 tcaactctag ttatctttat gccaattccc tccaagtcct taattttaat tgatctgcca
    24061 tgtgatctcc attttgaatg attcgttaat tctgcagcta tcttttttgc tctttcaagt
    24121 ttcatttgag cgtcaattgt acccaaactt ccagtttccg tcattaaaat agcttatatt
    24181 aactgtctca aaactaactt ttagtttctg aacagccacc atataattta aatcgtatct
    24241 tttttatttt gcttctctaa ttaatttgga ggttttctca atatcattta cattttcgta
    24301 tatcttcttt attggctgta attctgaaac ggaataatag taaattatat cttgaattag
    24361 atcttccaat agattaattg aaaatagagc atatctggtt tcttctaatg ggcttgatgt
    24421 taaagaggac ttataagaac catcatcttg ataaaaactc ttaattttaa ggattaattc
    24481 ttcaacttta ttgattagct gattaagttc gtttggtttt acatctattt tggaatagag
    24541 aaaatcaatg caatatacga ttttagtgat gtcttcaagt aagatatcct tatttatata
    24601 tgttagaaga taaataagcc ataaattggc attttgcgga atcttattta aatgatcaag
    24661 aataaagcag accttgaaaa aactatctaa atctttgggc attaacttta ctattttatc
    24721 ttcatcaatt acgccttgaa gtaagttgga caatttgtct ttattaatat ctagcaattg
    24781 atgttgaaaa ttgaggatga aatttttggc atctattctc ttacaaatgc ttttcaaatt
    24841 agtatccggc ttcagtatac ttaaaaattt atctttcgga actctataag cataagttcg
    24901 ccatgcctca accattccat attggctatt attaaagacc cattctgctt taaatattgc
    24961 atattgatat gaaggtttat aaatgtatat aataaattta tctttgatag gttcacgatt
    25021 gccttttttt gatttagcaa ttttggagac cttgaaatca taaaattcca aatctgcttt
    25081 ttcagcatat tgaaattcaa actctatttt tttatcgttt atttcagcaa tataatctgg
    25141 ctctcttgag atcccacctt ttcttaaaaa ccataagaat ttatccccac ccgatttata
    25201 aaccttaaca catttttcgg gatatagttc ttttatttta tcttccagcc attccaaaat
    25261 ttctaattca gcgatggctt ttttcttcat atctttgcga tattcaagat aagtgcggga
    25321 ttttatatca ctggaatggg cataaatggt ttttccattt aatttcataa cttgtccttt
    25381 ttaccaaatt tctgtttgat gatatcttag agcttttact gcttcataag caatctctgg
    25441 atatatttca tagccaatac cattgcgttt taaatctgct gcagcagaaa gagttactcc
    25501 tgaaccgaca aaaggatcaa gaattgtttc atccacataa ctaaatgcct taatgaaacg
    25561 gaagggaagt tcataaggaa aaggtgcaat atgatttcta ttatcaccgc ttccttgtgg
    25621 tttcataacc caaacatcgc ttttcttgat agacagccag aagtccttgt ccaacttgga
    25681 ttgttctttt tgctctttag taagatgacc atatttgtta aagcccttct tttccggttt
    25741 atcaaattcc aagatgaatt cgtgcatatt attgattaaa attccacctg gatagggata
    25801 agttccaaaa tgtgcccgga cggcatttgt tttttgccag ataatatctc ttttaaatat
    25861 aaagccaatt ttttcacaca aatctatggt tctacccgct aaattcaagg ttctaaactt
    25921 tcctttttct aatcggacag ggaggttcat tatatttata aatgcttttc tccctggttg
    25981 taatacccgg taaacttctt tccagacctc gcctattttc tcaaaccatt catcaatatt
    26041 atgaatattc ccaaggtcac caggaagtgg ctctcctgaa tacattttag tatcaaaata
    26101 gggtggagaa gtaatcatca gatggatgct gttatcctcc aaatcgtcca tcttcataga
    26161 attcttaata tatatttttt gaattgtgcc atttatctca agtgtattta ctttatcaat
    26221 atcaatgtta ttgctatgag atttatattt aagatttgca ctaaagtcct ttaactcttt
    26281 caggtcaaag cgcatttgtc cacttgcggc aatttgcgct ttaattagct gctgattgac
    26341 aatattatga agcatctcaa ctgttatacc taaaaatttg gatgcttcaa tagctgatag
    26401 ataagttcta tttttatagt ccgttggttc catttttgta tctcttttgc ttaatatcgt
    26461 gactaatgca ttatagatat aataggttac ttgcttctta agttttcatc ggcatcttac
    26521 cataacgaat ttacgaaaat ttaagctact attttatata taaggggtaa caaatctttt
    26581 ttttgtcacc tttggcgcaa actgctaaaa tatgttttat agttttaccg cttcggattt
    26641 tttattcctg aataaattca gccgcttctc tcataattta actaaaaatc atatataaac
    26701 cttaaaaaac caatgtaaaa tactgtcaat gaaatttcag cttaaaaaag caattatgat
    26761 attacaacag aaattcaaag ctaaaagcta atttgctgtt atataaaagg tgattaagct
    26821 ataatgttat tctcctgtat aagcacatac tccaacccgg ggagaattaa ctaccggtaa
    26881 taacatatcc actccacatt tatgtttgct ggcacattta tcacaaagcc atcctttgct
    26941 atcccaaata caagcaacac aaacttgagt ggcaggtttt ccacatacat aacatttttt
    27001 ctcaggagga tcatttctgg ccataataat aataaatctg cctttcgctt taagttccat
    27061 ttcggatata accttcaagg taagaaaagt ggttgtcccg aagtcgtatt tgtgataaaa
    27121 ttttatccct ggatatagaa cctcattcag cttaaatctc atactttttt cttttaattc
    27181 cggcattggc atcacggagt atgttttgtc ctcaatttta aaggcactaa ggtgtccgca
    27241 acattccagc catatatccc ttagaaaagt atccaacctt ttcagagata tatttgccgt
    27301 tactaaaaga tgcatccaat attcaggcaa gtatcttccc tcaaccaata aatgaaaaca
    27361 ctttacgctt tgtgtttcat catattccaa agatgactcc catgccggtt gcattgcctt
    27421 acaagatgcc aaatgcttgg tcattgctga cttactgaaa gttctaaagc aaaaatggca
    27481 tttacctgtt gattgaattc tgggcatttc tatgttgctc ctcttttata ttttgctatt
    27541 tctaataata tcgttgatta ctattcaaat gtcaaataat attttgatga taaaagagat
    27601 attccaaccc tgcttcaacg cctcaacgat attaatgata ttagacaagg catcttacta
    27661 taaaataaat aagagctact tgcgtagctc ttattattgg gctgatcatc aatattgcct
    27721 gctatttgac cagttcacat ctttcaacgc gggcaggcgc tccagatgat tcacctagat
    27781 attataatct tttttgtctt cttgccaact ctttttttct acaccttacc atcgccttca
    27841 tcatttgcgc tattttcttc tcttatcata aatt
//
LOCUS       DAOOZV010000020        27609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1896_length_27609_cov_56.029433, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000020 DAOOZV010000000
VERSION     DAOOZV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27609
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_27609_cov_56.029433"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1273)
                     /locus_tag="PLW08_03435"
     CDS             complement(374..1273)
                     /locus_tag="PLW08_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51689.1"
                     /translation="MNKITGTSTSSFGTKGRINHDSSKFYDSKLYEEIQTKKVIDKTE
                     NPFPEEYLNKVILGSAENMQEIPDNSVHLMITSPPYNVSKDYDKNLSLGQYLELLENS
                     FRETYRVLVNGGRACINVANIGRKPYIPLSDYISQIMINIGFNMRGEIIWNKASSASP
                     STAWGSWMSATNPILRDIHEYILIFSKGDYDRNKKGKQSTIRKEDFIEWTKSVWVMKA
                     ESAKKIGHPAPFPEELPYRLIQLYSFQDDIILDPFMGSGTTAISSLKSHRYFIGYEIE
                     PAYIELCNRRISTYLDQLTLELE"
     gene            complement(1273..1653)
                     /locus_tag="PLW08_03440"
     CDS             complement(1273..1653)
                     /locus_tag="PLW08_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51690.1"
                     /translation="MKITTKLYNNLGVLCVFGVLGVKNAELRIINNNQCKSAGVIQGF
                     WSLNPCPSEIDDYIKNSPRLGFKTYNNVTDIINDCEELVTNEYYFLSTMLNKPQIGKY
                     ISIAAREKTDTAKAEKFIELLKGK"
     gene            complement(1691..3583)
                     /locus_tag="PLW08_03445"
     CDS             complement(1691..3583)
                     /locus_tag="PLW08_03445"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPL51691.1"
                     /translation="MKTDIQDLINTIKTQANEARKAILTMTTLAGSGHPGGSMSSIDL
                     LLCVYNTIKHNPAQPQMPDRDRVIISNGHISPAVFSALGLLGYFDLDEAIAQYRLTGS
                     IFEGHTERRVPGVEWSSGNLGQGLSAGTGFALASRINKIPYNVYVFMGDGEQQKGQIS
                     EARRFAVKYCLNNIIAIIDYNQLQISGSIHNVMPQNIRKNWESDGWKVMEVDGHNIPL
                     ILTALDNARAFQQPTMILAHTIMGKGVTFMENNAQYHGSALSEAQLENALKELGTENE
                     LKKYRKLRSEFKPEHNPQISFAFEPDFTIGKPVRYETDSDCRSAWGKTIADLAKVNAN
                     AKTPIVVLDCDLATSVKTEEFAQVSPQNFFQCGIMEQHTCVMGGALSICGIQTFWSDF
                     GVFGIAEVYNNQRLNDINQTNLKTVVTHTGIDVGADGKTHQCIDYISLTRNLFGFKLI
                     FPADANQTDRIIRWLINKPGNYLVTMGRSKLPVLKKLDGTLQYDVDYNFIYGEADILR
                     DGNDGSMWVCGTPVAKAIKAVDTLCEEGIFLELLYVSSPLHLDLTQVKSSAGKGIIFS
                     VEDHSVNGGLGTSIADALAVNSLGAKLVKIGVTQYPISGEANELYHWAGLDTTSLIAT
                     VKKYLA"
     gene            complement(3668..3743)
                     /locus_tag="PLW08_03450"
     tRNA            complement(3668..3743)
                     /locus_tag="PLW08_03450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3708..3710),aa:Ala,seq:ggc)
     gene            4536..4613
                     /locus_tag="PLW08_03455"
     tRNA            4536..4613
                     /locus_tag="PLW08_03455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4571..4573,aa:Pro,seq:tgg)
     gene            4633..4708
                     /locus_tag="PLW08_03460"
     tRNA            4633..4708
                     /locus_tag="PLW08_03460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4666..4668,aa:Val,seq:tac)
     gene            4757..5728
                     /locus_tag="PLW08_03465"
     CDS             4757..5728
                     /locus_tag="PLW08_03465"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPL51692.1"
                     /translation="MKIAITGANGFVGSNLVKHFLSAGYEVKAIIRANAATTFIPSSA
                     QIVRVDYDDVTSLLTAFTGVDIIIHNAGKTKANNAEQMFKANLGITEKIIAASNRIAE
                     SQQIIYLSSQAASRPSINNIPVKEDELGAPITIYGKSKLAAEEAIKQKCKKYYIIIRP
                     CSVYGCGDKDFLQLFKIVDRGFSFQIGHKDKLLNMIYVSELCRFIELCVNNPSAYSQT
                     FFATDGQVYKQSDIFSAIAKALNKHPITITVPESLAKIVFYAGDLYGHLFHKEVVVNK
                     EKMKEILADAWLADISKAQNILGWQPIANLENNIKDTAKCYRALGWL"
     gene            5701..7389
                     /locus_tag="PLW08_03470"
     CDS             5701..7389
                     /locus_tag="PLW08_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51693.1"
                     /translation="MLPRIGLAIIFVLLFLPLSAEEILLEENISETDTISYTVHEIAK
                     TDIENWIKKNRIDTRESVFSTAPIHLDLGDLYHNCYFYLGRTNLPMPERNGFETFSGI
                     FSPALYHGSFNTLYPVKKYQRTVNYTNRTYPFAPVLTSVDAGLGDYDHHYARINFVKN
                     ELFSYSELEYQGDLLAQNGFWSDITSAETAMKHRLSRKGKLFNWEAEYASWKKDIAMN
                     YLLPFYWQLTNFKIDYELQQIYAELGIPWVKLKFLQESETASAREFYETYKRKSTRVQ
                     INTEQKFGFWQYQTLYEHSWNKDDKYFCGFYDEEKYEDKLALAFKIDNPLEIDLSGDW
                     LDWKRGRLFADIAYSLQNFEAGVTYKQLLGKDPSSLKVKDIFNSSGELELIDISIPRE
                     VSVQFSYNLWNIKPLIAVGSKEIKQKADSTFFNLEKQQPFLRLAMDANFKWKNWQLIA
                     RPEWVISKYYQNLTESPEYRFQSVQQIKYHLPYDNSVVAGCGIYGHSGYYIANAVNPS
                     LIEASTILDAWAGFDVSNYFELRAGIKNALSSAIYGAYSVPTSLYVQVLWLYLN"
     gene            7691..8491
                     /locus_tag="PLW08_03475"
     CDS             7691..8491
                     /locus_tag="PLW08_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPL51694.1"
                     /translation="MKLFETHAHLDHPDFDTDRETIINKCFSSGIEYIINVAFNKETA
                     YSSLELAKKHLHIFSTVGFHPHDAVDFDAEVVKKLAREKKVLAIGEIGLDFFRNLSPF
                     TVQREVFNNQAHLAVEYDLPIIVHDRDAHQECYNILKKNKVKEAVFHCFTGDIIFAQQ
                     VLDEGWMISFTANITYANSKLDNVIRLVPLDRFMVETDCPYLPPYLHRGKRNSPLFLP
                     LVVEKIAEVKEISPNEVAELSFANAYRFFRVPPDPVKTSHKKAGHKKA"
     gene            8556..9761
                     /locus_tag="PLW08_03480"
     CDS             8556..9761
                     /locus_tag="PLW08_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51695.1"
                     /translation="MKKIVIVICGIVLGISLWAGNSIFSFYGFPVRNYGRDIYSFGMG
                     DTGASDVFRLNTGYANPAMHNRNNYTLFSTGMILGYTKYQSRFEGEKHSYRDDSLDFP
                     YFNISIPINKHRLGVQFNSLSSGLVTNQRTLPDGSTETQTTDKYLYKADLIYSYSFKD
                     VNLGMSVNYYFGHNKLYFSQTGNYSNFDTYESLLMDFKNPSVTFGAIKIFDKYSIGAH
                     YNAPVTMKGELIRSCIHNTEPAVDYEYKLPELYCVSTTVKPLEQFKITADISYEPWES
                     ISHNYRDCYKAGIGFAYEPDTETHKHIFLKLPMRLGGSMRQLPFYDKNGNDIDEYAIS
                     CGLTLPLKNEVDRIDLGFQYLQRGALSTNNLSDTSMMLMIGFTGFDIISKGIDRTAPR
                     DIPEKEEVE"
     gene            9758..11320
                     /locus_tag="PLW08_03485"
     CDS             9758..11320
                     /locus_tag="PLW08_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPL51696.1"
                     /translation="MKQDEPNSNINISNDLLNQIIKSRNLTSLNLQTNLEMLPDEKLF
                     ANIDKVENRMRIALFENEPMVIFGHDDPDGITSTYILYNFLNSCGYQKHCYYIPNRNL
                     EPHGIQDGFIEFVRQNDYRLIVTVDNGIVSKEGVKNLNAMGCEVIITDHHIVQPDMLP
                     PAFTIMNPQLPECPYPFKQLAGVGVVLMLIRYLGRLWNHPVEPASYFWTAVGSIADKV
                     PMIGLNRILVRYVLEHFAEVQDETVTFLLRNYNRINTMSDIFSFLQYTSHLIANGREP
                     NGQHTAMRFILQLSDAKAILFEDLESQKNKWESELNRIFKFMDTLTTDFVGNWFVYYD
                     DEDVIPYTLLGTAATYVVSKLHIPAIILKYHNGVTVCEGRCGEDFNIMDAFTHCKKHL
                     AQFGGHPRAAGFTMKPEHYDAFLECFNSFLQKNYHPSKQEILSYDAEVCPKDLNWDNW
                     KKLEILLPWGQLNPEPSFLIRNTSRAEITRYVSLDNSGMDLPNKGKGDALVLWKAPNL
                     VKVLSWQQKINE"
     gene            11342..11929
                     /locus_tag="PLW08_03490"
     CDS             11342..11929
                     /locus_tag="PLW08_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPL51697.1"
                     /translation="MIEPGETLRKAIHISSLVIPLGYRYLLSFNRAWGFSLLLIAFTI
                     SLVIEFYRFWQKDFHKTFYRIFGILLRKHERKDFTGATYMLFSAVICVAFFNPLIASC
                     AMAFLTIGDTFAAFVGINFGKRRFNNGKKSLEGSLACFVSCLIFGIFWLDNPPLAIFG
                     ALTATLAELCNIPLDDNIKIPIASAVVMTLLSIVI"
     gene            11943..12935
                     /locus_tag="PLW08_03495"
     CDS             11943..12935
                     /locus_tag="PLW08_03495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL51698.1"
                     /translation="MKLSFIIPVLNEQESLKELYTEIVQNCQGYEYEIIFIDDGSVDN
                     SFAVMQELAENDEKVKAVKFRRNFGKATALQVGFNLAEGDIIFTLDGDLQDNPVEIPN
                     FIAKLNEGYDLVSGWKRKRRDPLYKILPSKLFNFVTAKTFHLKLKDYNCGFKAYRHPL
                     EKELVLYGEMHRYIPVLAHSLGYKVGEIPVAHRPRKFGKSKYGKERYLRGFFDLLTVK
                     MITQYSKSPLYLFGRVGIASTFIGLVISIYLAILKLFYGQPLSNRPLLFLGILLILGG
                     LQFISMGLISELIVNRFRQGNRTPVSITHCLNIESPVPLLESKQPFIELGDRKW"
     gene            12974..14197
                     /locus_tag="PLW08_03500"
     CDS             12974..14197
                     /locus_tag="PLW08_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL51699.1"
                     /translation="MDKLSTFFLTRYLQAPKRNLLRFSFVFMILGIILSVGILSAGLN
                     LFEGYERTLKDVLLGTFPHITLTKANLDNISNDETDSLITKLSKRDEIQQITPLLSYP
                     VMTAGKEKVRGAILNAYDFNSKQPFPQAKYIQKGKKIPAKGEVIIGKYLAKELGKDIG
                     DTLKVVFTRLDNISALGIFPSEYYLPIAGIYSSGFYETDRSLVLTNITDAEYMLNAKP
                     GFSKLEIRLKSKDIEKAPEIAQLLQTIAGPEYSVYPWQTFSAGLLHLVEMEKWLIFII
                     FCFLVLIAGINVISAVATIILDKKNEIAVLKTLGANSASIKRILCLQVGLSALLAIIA
                     GLVFGALLSLGIEKQNFYQLKGDVYFIDSLNTSIILINQIIIFVVASVLIFICIYFPL
                     KQIDKMEIIKLLRNP"
     gene            14247..14918
                     /locus_tag="PLW08_03505"
     CDS             14247..14918
                     /locus_tag="PLW08_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL51700.1"
                     /translation="MICLAGYDLCKSYTDSNQTINVLRKATLEVQESELVCITGKSGS
                     GKSTLLHLLGLLDSPDSGKVMIGGNYVQSTMPEAASIRNLELGFVFQFHYLIEDLTAQ
                     ENVALPMIISGVSHSKAIANAVSLLNELDLAERITHYPNQLSGGEQQRVALARALINN
                     PKIVLADEPTGNLDPEHSNEIWDMILKLNQERGQTFVIVTHDVANASKAQVTYNLSEG
                     KLEKR"
     gene            14934..18998
                     /locus_tag="PLW08_03510"
     CDS             14934..18998
                     /locus_tag="PLW08_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51701.1"
                     /translation="MPKAKKFLIFVLILLLINTGFFLAWYAFGLRNYCKNYLARYLGK
                     LTKGEVTIDELHISDRQLLAQGITYTSADSSIAVKVHRLSVQYNLTRYLFSGFKTSKL
                     ISDIEIYKPTVHYSYFYKPKPPKAKKPLVLPDFAHYFKRLQLTEGTAVISAEIPVKII
                     QEGYLSISDSFQGINLTAINKSTSDVTLNAFSAKGGKLTAKSLLDKGRLAKVDAEIAS
                     FRPLSVSHPDLQNLQTEISLVASLMQDTLKAPLKYKAEAQIWGTETLFAGNYPVLIPY
                     LWVKTDGRNLNLSLSHSNFGSSEMEGEVRISNLDQKMKFDYASATANIDLSMFHKELQ
                     GYVVVQAKGYGNIDKPELELNVTSTQAKYQKYAFRNLNLTGSYNNNELAFNLPELVFE
                     NQIINCSGNLNPLYRTINAHLETKPIKIMSQPYLATANLDVYAELWEKYPFIDATINQ
                     LDFNYDLATLQGVSGKVKLIPLASDNNLYLDANISGDNGFNIKVMGDIISRNLLLDVK
                     FDNLEVAELYSQPKVIELNPYLNGDIKAIITGENVLLQTLLGVKLDKPVPVNTPLDAL
                     GTFNYKTKEASLHLIGKNGMMNYQPLNFELSAMLKDNLLTVQGFKFNDFISLSGAINL
                     SNYRDLNFALNLKNIGYLDIVHYFPQLDINLPEFYGLNVFAEYNLENLGKLRANAYLS
                     YIDLLAVTPFSLKLELNGPVDSIQVAGNIHNSEQNILILSGNIGFQPELNISLDAGFQ
                     DLAIQNVIINSPLTGKLNGIAGITWHKIEERKPELELRANLLSEDITIKNFTINTAVI
                     QGKQTPQQLIVDSLFVQSAGLFKLNGSGAINYNALYNEFFEGPEQLNLNVEGELFGWL
                     KNLTPYILEAKGRSSLSISIGTSEDQFLVSSGKININNGFIRLKDQTEPLANINIRGG
                     FDKNRLIIEYGQVQMGEGKLIFNNIFEADNSDHFMLGFLDLGIFRAMCEEPGILVNIP
                     KYTTPRSLTKVILRGQNSRYATIRGPFDQMKISGEAVASNTNVLYPPDTENLLTMAST
                     VRGSTKKKTSEPNPLPFILDVKLIVGENVKYVTYPAELRVVPGGYLHLAYDGLSFFVK
                     EASFSSEQGTVDIFGTIFQVEKVNLTLVDSQNLLGLEGIFYKRAPDGSMITLSAITSS
                     DYTKSFMERLQLSLTSDNPEDKTISQILARLHYTGTETPDRTQGEFWQDEALGLISGN
                     LDASLFTPILSPIENYIRRTLHLDSFTINAGFIQNLYTEYTNNPQQFAEYTDMNQLSS
                     DIAKFSSSILLNNLSISMSKYLGSSIFLDYMLKLQEATNLQNRTRILVSNEASIRLIL
                     PKNYRVAYTVTYEAQEKKTSHEIMLSRFIRFWGL"
     gene            19222..19716
                     /locus_tag="PLW08_03515"
     CDS             19222..19716
                     /locus_tag="PLW08_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51702.1"
                     /translation="MPDNVKNENAVNTKKTPKMADFSTRVSLVELIMILMLVGLVFVF
                     YFGMKQLQIDKANEAIAQEKFENIIPTFKNIIEAMETYRKADEFGDYPAFLEELNLGD
                     INTNDFKFEYSADTYTITAITQPAFGKAGIKVIYNLSDKSFTVEDPIPDKKPTIKDEW
                     LPQD"
     gene            complement(20098..21027)
                     /locus_tag="PLW08_03520"
     CDS             complement(20098..21027)
                     /locus_tag="PLW08_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51703.1"
                     /translation="MSKYKEIDLKKIKTYPIDQRHSKVNINAFAKKGKVKVNDFFNCL
                     PDILAAKDLKELILACKQARENHKPVIIGLGAHIIKCGLAPLLIEMMEEDFVSAFVCN
                     GAVAIHDYEIASFGKTSEDVSSALSDGSFGMAEETVKGINEAINKGFKESLGLGEAIG
                     KELYTKAKYKDLSLLASAYKWEIPVCVQVAIGTDIIHQSPYADGKAIGDCSMRDFRIF
                     AEKVSELNGGGVFLNLGSAVIVPEVFLKALTVARNIYGEVQNFTTAVFDFNVHYRAKV
                     NVAERPVENGGKGYYFIGQNEIMVPLLLKAIME"
     gene            complement(20975..21556)
                     /locus_tag="PLW08_03525"
     CDS             complement(20975..21556)
                     /locus_tag="PLW08_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPL51704.1"
                     /translation="MAEKELLLHCCCAPCLIAPFYKLKAEGKELTAFWFNPNIHPLLE
                     YQKRRDTLRDFAGKENIELLEQDEYGLMNFLQTTLKDINNRCQYCYETRLNAVAKTAK
                     ELGYSSFSTTMLYSKYQKHELIVDIALKMSAKYGIDFFYEDWRSLWWEGRKLAKEAGL
                     YRQQYCGCIFSEEERYREQIQRDRPKENKDLSN"
     gene            complement(21701..22846)
                     /locus_tag="PLW08_03530"
     CDS             complement(21701..22846)
                     /locus_tag="PLW08_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL51705.1"
                     /translation="MDYFLTEEQLEMQEIARRIAEEKIRPVSEKYDEEGIFPWDIVEV
                     MKQSDLFAILVPEEYGGISGKVADLAVVTEELCAVDVGISLAFGATGLGMYPILIAGT
                     EEQKQKYLAQIAAGEQLAAFALTEANAGSDAGAIETTAKKEGDYYILNGTKQWITNGG
                     EAGIYCVFAMTDKTKGARGCSCLLVEKGTPGFNFGKKENKMGIRASATRELIFEDCKV
                     PAGNLIGREGTGFITAMKVFDKSRPMVGAQSVGVARGAFEAAIKYSKERQQFGKPISS
                     FQGIQFMLADMATQIEAARALVMQTARMIDSGARNYSKESAMCKYFASDVAMRVTTDA
                     VQILGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRNIVAASLLKEY"
     gene            complement(23351..24484)
                     /locus_tag="PLW08_03535"
     CDS             complement(23351..24484)
                     /locus_tag="PLW08_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPL51706.1"
                     /translation="MKFEEFKSGTYKNQFQYKSFLPSKINHQWTWDDPRINLLLEKAA
                     QTLGELNAFSRIVPNVDLFIRMHILKEANTSSRIEGTKTDIEDVVLDESSVYPEKRDD
                     WQEVQNYVQAMNEAIKQLENLPLSLRLIRNTHRILMTNVRGKHKTPGEFRTSQNWIGG
                     SSLADAAFIPPNKDDMSDLLDDFEHFLYNDRIVVPHLIRCAIAHYQFETIHPFQDGNG
                     RIGRLLITLYLVSNHLLIKPTLYLSDFFERHRGAYYDSLTRVRNSNDLGQWVRFFLEG
                     VIETAEKGKTTFAQILKLHTNIESELMNLGKKTDNARKLLMHLYQSPVVSVKQVESIL
                     GVKYYSANELIKTLVEINILEETTGYSRNRVFMFKRYIDVFKE"
     gene            complement(24898..26124)
                     /locus_tag="PLW08_03540"
     CDS             complement(24898..26124)
                     /locus_tag="PLW08_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51707.1"
                     /translation="MKKFFLLLLLPVICIAFLQADYIYVVNSESRTLSRIDTETGTVN
                     NSFTQLGLTPNMLKVNSDFIYVVLSGANSIQMIDRNSGTTIRNIFIASSSNPWDVVED
                     NGYLYVSGMFTNKVYKISQESWTVIDQITVGTAPEGLEVCNGKLYVCNTGGYASGYAN
                     SSVSVIDLDSFSVIKTIPVWTNPQDIAVFGSQLHIVCTGNWSSISGAVDIIDSVTDER
                     IDRLLIGGNPWSVWISPQGFAYLGEGYNSGVYSYNASTLEILHNSSNPLTPGGIMISG
                     STDCIAVLYAPYSQNGTVYLRNNNWQPITQWTVALIPTDICYYSNETPVSDEELPLPQ
                     ITLYPNPVNKDGILNFKADNAFYGELKIYNLKGQKVLQVPVQKGEARIDLKGKGMGSG
                     LYFYHSGKAKGKIIVK"
     gene            26311..26613
                     /locus_tag="PLW08_03545"
     CDS             26311..26613
                     /locus_tag="PLW08_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51708.1"
                     /translation="MIILGKAREKMELKDKIIPVDELIPVIEDIGAISFYNIVKDYIS
                     GNYNTSAFEPDDDLDCRDPFLQQAYEHNLLSDSQIKEIYEIDEFVSLTRFPGIIRI"
     gene            complement(27102..>27609)
                     /locus_tag="PLW08_03550"
     CDS             complement(27102..>27609)
                     /locus_tag="PLW08_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL51709.1"
                     /translation="QPSNSAPQRIESLIITDRYQKSCKAEFPGCYVIETNYTDFSAEE
                     IWKFYMNLSEVEAAFRSMKTELGTRPIYHQKDGRIEVHLFYSVLAYAILKSITYKLAL
                     EDVHISWQSIKQILQTYMCSDTIFNTTDVYRVRNKQTAQPEAKAKKIYSLLNIKVSKN
                     TVSQKYRL"
BASE COUNT         8525 a         5340 c         4934 g         8810 t
ORIGIN      
        1 cttaaccagg gaacgattga gacaatccga ctgtaacttc ttatttcaca aagaggaaag
       61 agagcaatgt ttaatgaaga attgagaatt gaaaattata aatcttttct aatatatatc
      121 tgtgttatcc gtgaaatctg tgagagaaaa ttctatgtct ctataagtca taagcgcctt
      181 caaagatatc aaccagctaa aaagttatca aggcgcaaat gactcctgtc ttacaatgca
      241 aataaatcct aactttcatt ctcaattctc cattttcaat agtcaattaa tcctcttttt
      301 ctcttgccct ctttgtaaaa taatcctctt tttctctttg ttcccctttt tatcatttca
      361 ttctttttac tttctattcc aattccagag ttaattgatc caaataagtg ctaatccttc
      421 tattgcatag ctctatataa gcaggttcaa tttcataacc aataaaatag cgatgtgatt
      481 ttaaagaaga tattgcagtt gttccgctgc ccataaaagg atcaagaatt atatcatcct
      541 ggaaggaata taattgtatt aaacggtaag gaagttcttc aggaaaaggt gccgggtgtc
      601 caattttttt tgctgattcc gctttcataa cccacactga ttttgtccat tctataaagt
      661 cctcttttcg gatcgtgctt tgtttgcctt ttttgtttct atcataatct cctttggaaa
      721 aaattaaaat atattcgtga atatccctta aaataggatt ggtagcactc atccagcttc
      781 cccaggcagt agagggactg gcacttgatg ccttattcca aattatttca cccctcatat
      841 taaaaccaat gtttatcata atttgggaga tataatcgga cagaggaata taaggtttcc
      901 ttccaatatt agctacatta atacaagctc ttccaccatt aactaagacc ctgtatgttt
      961 ccctgaaaga attttctaac aattccagat attgtcccag ggataaattc ttatcataat
     1021 ccttgctaac attgtaaggt ggtgaggtta tcattaaatg aacagaatta tcaggaattt
     1081 cctgcatatt ttcagcagaa ccaagaataa ccttattcaa atattcctca gggaatggat
     1141 tttctgtttt atctatcact tttttagttt gtatctcttc gtataattta gaatcataaa
     1201 acttggaaga gtcgtgattt attcttccct ttgtgccaaa tgaactggta gatgttcctg
     1261 ttatcttgtt cattatttgc ctttaagaag ttcaatgaat ttctctgctt tagcggtatc
     1321 ggttttctct cttgccgcta tacttatgta tttgccaatt tgtggcttat ttaacatagt
     1381 agatagaaaa taatattcgt tagttacgag ctcttcgcaa tcattaataa tatcagttac
     1441 attgttatag gttttaaatc ccaaccgggg tgagttcttt atataatcat caatttctga
     1501 tgggcaagga tttagagacc aaaaaccttg aatcacaccg gcagatttac actgattatt
     1561 atttataatt cttaattctg cattctttac accgagaaca ccgaaaacac agagaacacc
     1621 gagattatta tataattttg tagttatctt cattcacaat tttcaattct caatttttca
     1681 ttcttcattt ttatgccaga tattttttta ccgtagcaat aagtgacgta gtatccaagc
     1741 cagcccaatg gtagagttca tttgcttctc cggaaatcgg atattgtgta accccgattt
     1801 taactaattt tgcacccaga gaattgacgg caagagcatc ggcaatagag gttcccaagc
     1861 caccatttac agaatgatct tccacactga aaattatacc tttaccggca gaggacttta
     1921 cttgagtcag gtcaagatgc aagggtgagg aaacatagag caattcaaga aaaatacctt
     1981 cttcgcataa ggtatcaact gcctttatcg ctttggcaac aggagttccg caaacccaca
     2041 tactgccgtc atttccgtct ctaagaatat ctgcttcacc gtaaataaag ttataatcca
     2101 catcatattg caaagttccg tctaattttt tcagcacggg aagtttggag cgtcccatcg
     2161 ttaccagata atttcctggt ttattaatca accagcggat aattctatca gtttgattgg
     2221 cgtctgcagg aaagataagt ttaaagccaa agaggtttct ggttaaagaa atatagtcaa
     2281 tacactgatg ggtctttccg tctgcaccta catctatgcc tgtatgtgta acaactgttt
     2341 tcagattggt ttgattgata tcgttaagac gttgattatt atagacctct gcaataccga
     2401 agacaccaaa atcactccag aaggtctgaa ttccacaaat agaaagagct ccccccatca
     2461 cgcaagtatg ctgttccata atcccgcact ggaaaaagtt ttggggactt acctgggcaa
     2521 attcttccgt ctttacgctt gtcgccaaat cgcaatccaa aaccacaatt ggcgttttgg
     2581 cattagcatt tactttggct aaatcggcaa ttgtttttcc ccaggcgctt ctgcaatcgc
     2641 tatctgtttc ataccgaacg ggcttgccta ttgtaaaatc gggctcaaat gcaaaactga
     2701 tttgcgggtt atgttccggc ttaaattccg agcgcagttt acggtatttt ttcagctcat
     2761 tctctgttcc caattccttc aaggcgtttt ccaattgagc ttcggacaaa gcagaaccgt
     2821 gatattgggc attattttcc ataaaagtca cgcctttacc cattatcgta tgtgccagaa
     2881 tcattgttgg ttgctgaaaa gcgcgtgcat tgtcaagggc agtaagaata agaggaatat
     2941 tgtgaccatc aacttccatc actttccagc catctgattc ccagtttttt cggatatttt
     3001 gaggcataac attatggatg gaaccgctta tctgcaattg attatagtca ataatagcga
     3061 taatattatt taggcaatat tttactgcaa agcgtcttgc ctcgctaatc tgaccttttt
     3121 gctgttctcc gtcacccata aaaacataaa cattgtaggg aattttgttt atccgggaag
     3181 caagtgcaaa gccagttccg gcagataaac cctgacctaa attcccacta ctccattcca
     3241 cccccggaac tctgcgttcc gtatgacctt caaaaatact tcccgtaagc cgatattgag
     3301 cgattgcttc gtccagatca aaatagccca gaagtccaag agcactgaaa acagcgggag
     3361 aaatatgtcc attgctgatt ataactctat ctctatctgg catttggggt tgagcaggat
     3421 tatgttttat tgtattatac acgcacaaaa gaaggtcaat tgaggacatt gaaccgccag
     3481 gatgacccga accggcaaga gttgtcattg ttaaaattgc ttttctggct tcatttgcct
     3541 gtgttttaat agtattaatc aagtcctgaa tatcggtttt catttgtttt ccttatcttc
     3601 tatttttctc ttgcaaaaaa gtttatcccc ttcaccaatt cattttttga tgaaggggaa
     3661 taaaaaatgg tggagcatag cgggatcgaa ccgctgacct catgactgcc agtcatgcgc
     3721 tctcccagct gagctaatgc cccaacagaa aaatcacttt atatgagatc attgcctgca
     3781 aagggcagaa ttctgtcaag cagaaactac agcattttta ctttattatt ctgtaactta
     3841 ctgtcggatt gtcaattgaa aaactaatat ttctctatat taactacatt gggctagtaa
     3901 tgattttttt tcacctttta acgggaaaat aaagggtgga aagttttttc tttactctgg
     3961 aaactaaaga ttgcttatgg aatttatact tcaggcaatt tctatctttg ctaaaaccag
     4021 cataatatat acaaactttc ctatcaatcc ttagccaaaa gcgttaatct ggccttaatc
     4081 aagcgttaat caagcgttaa taattaacgc ttgattaagg tatgattaac ccttgattaa
     4141 cagtttcacc ttataacaaa cttgaggttg aaaaagtgac atcttcccca cattctacct
     4201 ccaattccac atttccctta ccactaattt ttgcggtaag ggggcaaaat aacggataaa
     4261 ctgaattatt tccgcttaca tccaacccag cattatcgtt ttacctaacc tttcgctaag
     4321 gaatcaaaat aagggagata cggcgtcctc gccctctaca tccaatctgg cagttccctt
     4381 ccaacaaaca ttttggtaac acaatagctt ggcagaaggt taaacaatga gaatagtgtt
     4441 ttccctaaaa ttaagcggag gaacagcgtc ctccgtctac acaacgaaag ttattgacaa
     4501 aatttactct gtccaagttt tggattttag acaaacgggg tgtagcgcag cccggttagc
     4561 gcacctgctt tgggagcagg gagtcggagg ttcgaatcct ctcaccccga ccaatgtaat
     4621 atattgtggc ttgggcgctt agctcagttg ggagagcaca tgctttacac gcatgtggtc
     4681 gggggttcga gtcccttagc gcccaccatt tttattttat agtttggaaa tagataaata
     4741 cgataagtgg tgaggaatga aaatagcaat cacaggagcc aacggatttg taggcagtaa
     4801 tctggttaaa cactttcttt ctgctggcta tgaagttaag gcaataatcc gggctaatgc
     4861 tgccactaca tttattcctt ccagtgcaca aatcgttagg gtagattatg atgatgtaac
     4921 ttcactcctt accgctttta ccggggtaga tataataatc cataatgccg gaaaaacaaa
     4981 agcaaataat gcggaacaga tgttcaaagc caatttgggt attacggaaa aaattattgc
     5041 agcaagcaat agaattgccg aatcacagca gattatttat cttagttcac aggcagcatc
     5101 gcgtcccagt attaataata ttcccgtaaa agaagatgaa ttaggagcac caataacaat
     5161 ttatggtaaa agcaaactgg ctgcagaaga ggcaattaag caaaaatgta aaaagtatta
     5221 tatcattatc cgtccctgtt ctgtttacgg ctgtggagat aaggactttt tacagctttt
     5281 taaaatagtg gatagaggat ttagtttcca aatagggcac aaagataaac tgctgaatat
     5341 gatttatgtt tccgaattat gtcggtttat tgaactctgt gtaaataatc cctctgccta
     5401 ttctcagacc ttttttgcta ccgatggaca ggtctataag cagagcgata ttttttccgc
     5461 tattgccaaa gccctgaata aacatcctat aacaattact gttcccgaaa gtttagccaa
     5521 aattgtattt tatgcgggag acctttatgg gcatcttttc cacaaagaag tggtagttaa
     5581 taaggaaaaa atgaaggaaa ttctggctga tgcctggctc gcagatatct ctaaagctca
     5641 aaatattctt ggctggcaac ccatagccaa tctggaaaat aatattaagg ataccgcaaa
     5701 gtgttaccgc gcattgggtt ggctataata tttgttctgc tgttccttcc tttgagcgcg
     5761 gaagaaatat tgttagagga aaatatatct gaaaccgaca caataagtta caccgtccac
     5821 gaaatagcta aaacagatat tgagaattgg ataaaaaaga acagaattga tactcgtgaa
     5881 tctgttttca gcactgctcc tattcattta gacctgggtg atttgtatca taattgttat
     5941 ttctatttgg gaagaaccaa tttaccgatg ccggaaagaa acggttttga gacattttcc
     6001 gggatatttt cgcctgcttt gtatcacggt tcattcaata cactgtatcc ggttaaaaaa
     6061 tatcaaagaa cggtaaatta taccaataga acttatcctt ttgctcctgt tctaacttct
     6121 gtggatgcag gactgggtga ttatgatcat cactacgcca gaattaattt tgttaaaaac
     6181 gagctgtttt cttattcaga attggaatac caaggtgatt tactggctca aaacggtttc
     6241 tggtcggata taacctctgc agaaacagca atgaaacatc gtctttcccg gaaaggaaaa
     6301 ctatttaact gggaagctga atatgcaagt tggaaaaaag atattgcaat gaattatttg
     6361 ctcccttttt attggcaact taccaacttc aaaattgatt atgaattaca gcagatttat
     6421 gcggaattgg gaatcccctg ggtaaaactg aaatttttac aagaatccga aactgcttct
     6481 gccagagaat tttatgaaac atacaaacgt aagtccacaa gagttcagat taacacggaa
     6541 cagaaattcg gtttttggca atatcaaact ttgtatgaac atagctggaa taaagacgat
     6601 aaatacttct gcggattcta tgatgaagaa aaatatgaag ataaacttgc cctcgctttt
     6661 aagatagata acccgctgga aattgactta agcggtgact ggcttgactg gaaacgggga
     6721 agattatttg ccgatattgc atattctctt caaaactttg aagcgggagt aacctataaa
     6781 caacttttag gcaaagaccc ttcttccctg aaggttaaag atatttttaa ttcctcggga
     6841 gaattggagt tgatagatat atccattccc cgagaggtat ccgttcaatt cagctataac
     6901 ctttggaata ttaaaccctt aatcgctgtg ggcagcaaag aaattaaaca aaaagcagac
     6961 agcacttttt ttaatttgga aaaacaacaa cccttcttgc gcctagcaat ggatgctaat
     7021 ttcaaatgga aaaattggca gttaatcgcc agacctgaat gggtaataag caaatactat
     7081 caaaacttga ccgaaagtcc cgaataccga tttcaaagtg tgcaacaaat aaaataccat
     7141 ttaccttacg ataattcggt agttgccggt tgcggtatct atggccactc tggatattat
     7201 attgccaatg ccgtaaaccc atctttaatt gaggcatcaa caattttgga tgcctgggct
     7261 ggttttgatg taagcaacta ctttgaatta cgtgcgggaa ttaaaaacgc tctttcttca
     7321 gcaatttatg gtgcctattc ggttccgaca tcactctatg tgcaagttct ttggctctat
     7381 ttgaattaac gaaaaaacaa aaagtgctgg agtattaaag tactggagtg ctggagtgct
     7441 gaagttcgta agttatataa atatcaaatt gaaaatgcat aaatattatt ttaccttatg
     7501 tagattcctg cttccacagg aataataaat ggctcatcat tccaaaaatg atcttatacc
     7561 ttatttcgga gctctttatg atttcagtgt cctcggtgta aaaaaatcgg tgctgttgat
     7621 gtgcttgaca aaaatacgct atttattttg aattgaattt atcttataaa tgaggtttaa
     7681 taattgaaaa atgaaattat ttgaaacaca tgcccatctg gatcatcccg attttgatac
     7741 cgacagagaa accataatta acaaatgctt tagcagtggc attgaatata tcattaatgt
     7801 tgctttcaat aaagaaacag catatagttc tttggagctg gctaaaaagc atttgcacat
     7861 cttttctact gttggttttc atcctcatga tgcagtggat tttgatgccg aggtggtaaa
     7921 aaaactggca agagaaaaaa aggtcttggc tatcggggaa atcggactgg acttttttcg
     7981 gaatttatct ccttttacgg ttcaaagaga ggtttttaat aatcaggcac atttagctgt
     8041 agaatatgat ttgccgataa ttgttcacga cagagatgcc catcaggaat gttataacat
     8101 tctgaaaaaa aataaagtta aagaagctgt ctttcattgt tttacgggtg atattatttt
     8161 tgcacaacag gttttggatg agggttggat gatttctttt acggctaata tcacttatgc
     8221 taattccaaa ttggataatg ttattcgttt agttcccttg gatagattta tggtggaaac
     8281 ggattgtccc tatttgcctc cctatttaca ccgcggaaaa agaaattcac ctcttttcct
     8341 gcctttagtt gtagaaaaga tagcagaagt taaagaaatc agtcccaatg aagtagcaga
     8401 actctctttt gcgaatgctt atcgtttttt ccgtgttcct cccgaccctg taaaaacatc
     8461 acataaaaaa gccggacata aaaaagctta atgttatata taggttaact aattagccgg
     8521 caagatatta tgactttaaa gtgaaaggat atataatgaa aaaaatagtt atcgttatct
     8581 gtggcatagt attaggtata tcactttggg caggaaattc catcttctcc ttttatggtt
     8641 ttcctgttcg taattatggc agagatattt acagttttgg tatgggtgat accggtgcca
     8701 gtgatgtttt tcgtctcaat accggttatg caaatcctgc aatgcataat agaaataact
     8761 acactttatt ttctaccgga atgattttgg gctatactaa atatcaatcc cgttttgaag
     8821 gcgagaaaca tagttatcgg gatgattccc tggattttcc ctattttaat atcagtatac
     8881 ctattaacaa acaccgtttg ggagttcaat tcaattctct ctcttcagga ttggttacga
     8941 atcaaagaac ccttcccgat ggttcaacgg aaacccaaac caccgacaaa tatctctata
     9001 aagccgatct gatttacagt tacagtttta aagatgtaaa cttgggaatg agtgtaaact
     9061 attatttcgg acataacaaa ctttattttt cgcaaacggg aaattattcc aattttgata
     9121 cttatgaatc cctgctgatg gatttcaaga atcccagcgt aacttttgga gcaattaaaa
     9181 tttttgataa atacagtatc ggtgcacatt acaatgctcc cgtaacaatg aaaggtgaac
     9241 tcattcgcag ctgcattcat aataccgaac cggctgttga ttatgaatat aaactgccgg
     9301 aactttattg tgtaagcaca accgttaagc ccttggaaca atttaagata actgcagata
     9361 tcagctacga accctgggaa tcaatttccc ataactatag ggattgttat aaagcaggaa
     9421 tcggatttgc ttatgagcca gatactgaaa ctcataaaca tatttttctg aaactgccta
     9481 tgaggttagg tggctctatg cgtcagctcc ctttttatga taaaaatggc aatgatattg
     9541 atgaatatgc catcagctgt ggacttaccc ttcccttgaa aaatgaagta gatagaattg
     9601 atttagggtt tcaatattta caacgaggtg ctctttccac caacaatctt agcgatactt
     9661 caatgatgct gatgatcgga tttaccggtt ttgatattat cagcaaggga atagatagaa
     9721 ctgctccgcg tgatatacct gaaaaagagg aagttgaatg aagcaagatg aacctaatag
     9781 taatataaat atatctaatg atttattaaa tcagattatt aaatcccgta accttacttc
     9841 cctgaatttg cagaccaatc ttgaaatgct acccgatgaa aagctttttg ccaatattga
     9901 caaggtggaa aataggatgc ggattgctct ttttgagaat gaaccgatgg tgattttcgg
     9961 tcacgatgat cctgatggaa taaccagcac ctatattttg tataacttct taaattcctg
    10021 cggttatcaa aaacattgtt attatattcc caatcgcaat ctggagcccc atggcattca
    10081 ggatggtttt attgaatttg tgcgtcaaaa tgattaccgg ttaatagtta cagtggataa
    10141 cggcattgtt tccaaagaag gggtaaaaaa tctgaatgca atgggttgtg aagtaataat
    10201 aaccgatcat catattgtgc agccggatat gttacctcct gcatttacga taatgaatcc
    10261 tcagctgccg gaatgtccat atccttttaa acagcttgcc ggagtaggag ttgttttaat
    10321 gctgattaga tatttgggac gcctttggaa tcaccctgtt gaacctgcca gttacttttg
    10381 gactgctgtt ggctctattg ctgataaagt tcctatgatt ggcttgaata gaatccttgt
    10441 tcgctatgtt ttagaacatt ttgctgaggt tcaggatgaa acagttactt ttcttctgcg
    10501 taattataac cgtatcaata caatgagtga tattttcagc tttttgcaat atacttccca
    10561 tcttatagct aatggtcgtg aacctaatgg ccagcatact gcaatgcgct ttattctgca
    10621 gttatcagat gccaaagcta tactttttga ggacttggaa agccaaaaga ataaatggga
    10681 aagcgaattg aacagaattt tcaaatttat ggatacccta acaacggatt ttgtaggtaa
    10741 ctggtttgtt tattatgatg atgaagatgt tattccttac actcttttgg gaacagcggc
    10801 aacttatgtt gtaagcaaac tgcatattcc tgctataatc ctaaaatatc ataatggcgt
    10861 tacagtttgc gaaggcagat gtggagagga ttttaacata atggatgctt tcactcactg
    10921 taaaaagcac ttagcacaat ttggaggtca tcccagagca gcaggattta caatgaaacc
    10981 tgaacactac gatgcttttc tggagtgttt caacagcttt ttgcagaaga attatcaccc
    11041 ttctaaacag gaaatattaa gctacgatgc agaggtatgt cctaaagacc tgaactggga
    11101 taattggaaa aagctggaaa ttttgttacc ttggggacag ttgaatccgg aaccttcatt
    11161 tttaattcgt aacacttcca gagctgaaat tacccgttat gtatctttgg ataacagcgg
    11221 tatggatttg ccgaataagg gaaaaggcga tgcgttagtt ttgtggaaag ctcccaatct
    11281 ggttaaagtg ttatcctggc agcaaaaaat caatgaataa attaacagga ggcatctgca
    11341 gttgattgag cccggtgaaa ccctaagaaa agcaatacat atcagcagtt tagttatccc
    11401 cttgggttat cgttatctgc tgtctttcaa tcgtgcttgg ggtttttcac tcttgctgat
    11461 tgccttcact attagtttgg taatagaatt ttaccgtttt tggcagaagg attttcataa
    11521 gaccttttac cgcatctttg gtatcctgct tagaaagcat gagcgcaaag attttacggg
    11581 tgctacttat atgctttttt cagcagtgat ttgcgttgct tttttcaatc cgcttattgc
    11641 ttcctgcgct atggcttttt taacgattgg cgatactttt gctgcttttg taggaattaa
    11701 tttcggtaaa cgtcggttta acaacggaaa aaaaagtttg gaaggtagtt tagcttgttt
    11761 tgtcagttgc ttaattttcg gcatcttttg gctggataat cctccactgg ctattttcgg
    11821 tgctttaact gctacattag cagaattatg taacattccc cttgacgaca atatcaagat
    11881 tccaattgct tcagctgtag taatgacttt gctgagcatt gtaatttaaa gataaggtag
    11941 ctatgaaatt atcctttata attccggtct tgaatgaaca ggagtcctta aaagaactct
    12001 atactgaaat tgtgcaaaac tgccagggct atgaatatga aatcatcttt attgatgatg
    12061 gctctgttga taacagcttt gctgttatgc aggaattggc agaaaacgat gaaaaagtta
    12121 aagcagttaa gttcagacgc aattttggca aagctacagc tcttcaggtt ggttttaacc
    12181 ttgccgaagg agatattatt tttaccctgg atggcgattt acaagataat cctgtggaaa
    12241 ttccgaattt tattgctaag cttaatgagg gctacgatct ggtttccggt tggaaacgca
    12301 aaagaagaga tccgttatat aaaatcctgc cttctaaatt gttcaatttc gttactgcta
    12361 agaccttcca cttgaaatta aaggactata attgcggatt taaggcatat cgtcatcctc
    12421 tggaaaaaga acttgtcctc tatggagaaa tgcatcgtta catacctgtt ttggctcatt
    12481 ccctgggcta taaagttggc gaaattccgg ttgctcatcg tccgcgtaaa ttcggtaaaa
    12541 gcaaatatgg taaagagcgt tatttgcgcg gtttttttga ccttctaacc gtaaaaatga
    12601 ttacccaata cagtaaaagt cctttgtatc tctttgggcg cgttggaatt gcctccactt
    12661 ttattggtct tgtcattagt atttaccttg ctattttgaa attgttctat ggtcagccgc
    12721 tttccaatcg tcctcttttg ttcttaggca ttttacttat tttaggtggt ttacagttta
    12781 tttctatggg cttgatttca gaattaattg taaatcgttt ccgtcaagga aacaggacac
    12841 ctgtttctat cactcattgc ctgaatattg agtccccggt tcccttgttg gaatccaagc
    12901 aaccctttat agaattagga gacaggaaat ggtgagaaca tcattttcca tctcgccatc
    12961 tcactaatta gttatggata agctttcaac ttttttccta acacgctatc tccaagctcc
    13021 caagcgtaat ttattacggt tcagttttgt gtttatgatt ttgggaatta tcctttctgt
    13081 aggaatttta agtgccggtt tgaacctctt tgaaggttat gaaaggactt tgaaggatgt
    13141 cctcttggga actttccccc acatcacttt aactaaagca aatctggata atatatccaa
    13201 cgatgaaacc gattcgctga taactaaact ttctaaaagg gatgaaattc agcaaattac
    13261 tcctctgctt agctaccctg ttatgacggc tggaaaagaa aaagtgcggg gtgcaattct
    13321 caatgcttat gattttaaca gtaaacaacc ctttccgcag gcaaaataca ttcaaaaagg
    13381 caaaaaaata cctgccaagg gagaagttat tattggtaaa tatctggcta aagaactggg
    13441 taaagatatt ggagatactt taaaagttgt ctttacccgt ttagataata tttccgcttt
    13501 gggcattttt ccttcagaat attatttgcc gatagcaggc atttacagca gcggatttta
    13561 tgaaacagac cgctctttag ttcttaccaa cattacagac gccgaatata tgctaaatgc
    13621 aaaacccggt ttcagtaaac tggaaatacg cttaaaatca aaggacatag aaaaagctcc
    13681 cgaaattgct caactcctcc aaacaattgc aggtcccgaa tattcagtat acccttggca
    13741 gaccttcagt gccggtttat tacatctggt agaaatggaa aaatggctga tttttattat
    13801 tttctgtttt ttggttttaa ttgccggaat aaatgttatt tctgctgtcg caaccatcat
    13861 tctggataaa aagaacgaaa ttgccgtcct taaaaccctg ggagccaatt ctgcctccat
    13921 taaaagaata ctttgtctac aggtagggct ctctgcttta ttggctatta tagcaggtct
    13981 ggtttttggc gcacttcttt ccttgggtat agaaaagcag aatttttatc aactgaaagg
    14041 tgatgtttat tttattgaca gcttaaacac gagtatcatt ctcataaatc aaataatcat
    14101 ttttgttgtt gcctcggttc tgatatttat ttgtatttat ttccctctta agcaaataga
    14161 taagatggaa attattaaat tactgcgcaa cccataacgg attatattat gaaaaagcga
    14221 ttgttttatt ttataaggat tttggtatga tttgtttagc cggttacgac ctctgcaaaa
    14281 gttacacaga cagcaatcag accatcaatg ttttacggaa agctactctt gaagtgcagg
    14341 aatccgaatt ggtctgtatt acaggaaaat ccggctcggg aaaaagtact ctcctccatc
    14401 ttttgggttt actggattcc ccggattccg gaaaagtaat gattggtgga aattatgtgc
    14461 agtccactat gccggaagct gcttccatac gcaatttaga gctgggattt gttttccagt
    14521 ttcactacct gatagaagac ctcacagctc aggaaaatgt agctttaccg atgataatat
    14581 ctggtgtaag tcattccaaa gcaattgcta atgctgtttc acttctaaat gaacttgatt
    14641 tggctgagcg cataactcat tatcccaatc aattaagtgg tggcgaacag caacgggttg
    14701 ctcttgccag agctttgatc aataacccta aaatcgtttt ggcagatgaa ccgacaggga
    14761 atcttgaccc ggaacacagt aatgagattt gggatatgat tttgaagctc aatcaagaac
    14821 gcggtcaaac cttcgttatc gtaactcatg atgttgctaa tgcctctaag gcacaggtaa
    14881 cttataattt aagcgaaggc aaactggaaa aaagataaac tggtcattct gctatgccaa
    14941 aagcaaaaaa gttcctgatt tttgtgctga ttctcctgtt gataaacacg ggcttctttc
    15001 ttgcctggta tgcttttggt ttaagaaatt actgcaaaaa ttatctcgcc agatatttgg
    15061 ggaaattgac caagggtgag gtcactattg atgaattgca tattagtgac cgtcagttat
    15121 tagcacaagg tattacttat acttcagctg atagtagtat tgccgtaaag gttcatcgct
    15181 taagtgttca gtataatcta acgcgttacc ttttttccgg ttttaaaacc agtaagttaa
    15241 ttagtgacat agaaatatac aagcccacgg tacattacag ttatttttat aaacccaaac
    15301 ctcctaaagc caaaaaacct cttgttcttc ccgattttgc tcattatttt aagcgtttgc
    15361 agttaactga aggaacggct gttatttctg ctgaaatacc ggttaaaatt atacaggaag
    15421 ggtatttaag catatcagac agttttcagg gaattaacct gaccgctatc aataaatcca
    15481 cttccgatgt taccttaaat gcctttagtg ccaaaggcgg aaaattgacc gctaagagct
    15541 tattagataa aggacgcttg gctaaagtgg atgctgaaat tgcttctttc cgacctcttt
    15601 ccgtttctca tcccgattta caaaatctgc aaacggaaat ttccttggtt gcctccttaa
    15661 tgcaagatac cctaaaagct cctcttaaat ataaagctga agctcaaatt tggggaacgg
    15721 aaacattatt tgccggaaat tatcctgttc taattcccta tctttgggtt aaaaccgatg
    15781 gtagaaacct aaatttatcc ctttctcaca gcaatttcgg cagtagtgaa atggaaggag
    15841 aggttagaat aagcaattta gaccaaaaaa tgaagtttga ttatgcctct gccactgcca
    15901 atattgattt gagtatgttc cataaggaac ttcagggcta tgtagtggtg caagctaaag
    15961 gatatggcaa tatagataaa cccgaattgg aactaaatgt tacctccacc caggcaaaat
    16021 atcaaaaata tgcttttcgc aatcttaatt taactggttc ctacaacaat aatgagcttg
    16081 cttttaattt accggaactc gtttttgaga atcaaattat taactgttca ggtaatctaa
    16141 accctcttta cagaacaatt aatgctcatc tggaaacaaa accgataaaa ataatgtccc
    16201 aaccctatct ggcaactgct aatttggatg tttatgcaga attatgggaa aaatatcctt
    16261 ttattgatgc cactatcaat cagctggatt ttaattatga cctggcaacg cttcaaggag
    16321 taagcggtaa agtaaaatta atacctcttg ccagtgacaa taatctatat ttagatgcca
    16381 atatatcagg agataacggt tttaatatta aagttatggg tgatattatt agtcgtaacc
    16441 ttctattaga tgtaaagttt gataacttgg aagtggcaga actctattct caacccaaag
    16501 ttattgaact gaatccctat ctgaatggag acataaaagc aatcattacc ggtgaaaatg
    16561 tcctgttaca aacactttta ggagtaaaat tagataaacc tgtgcctgta aatacccctt
    16621 tagatgcctt gggaaccttt aattataaaa ccaaagaggc ctctttacat cttatcggca
    16681 agaatggaat gatgaattat cagcccctaa attttgaact ttctgcaatg ctgaaagata
    16741 acctgcttac cgttcagggt tttaagttca atgacttcat cagtttaagc ggagcaatta
    16801 atctctccaa ctatcgggac ctgaattttg ccttaaatct taaaaacatc ggttatttgg
    16861 atattgtgca ttattttcct cagctggata tcaatctgcc agaattttac ggactaaatg
    16921 tttttgccga atacaatctt gaaaatttgg gaaaactgag agcaaatgcc tatttatctt
    16981 atattgacct tttggcagtt actccttttt ctctgaagtt agaattaaac ggtcctgtag
    17041 atagtattca ggttgcgggt aatattcaca attcggaaca aaacattctg attctaagcg
    17101 gtaatattgg tttccaaccg gagctgaata ttagtttgga tgctggtttc caagacctcg
    17161 ctattcaaaa tgttattatt aattctcctt taacaggaaa attaaatgga atagcaggaa
    17221 ttacctggca taaaattgaa gaacgaaaac cggaattgga actgagggca aaccttcttt
    17281 cggaagacat cactataaaa aattttacca tcaatacagc tgttattcaa ggtaaacaaa
    17341 caccccagca actcattgta gacagtttat ttgtgcaatc ggcaggactt tttaaactta
    17401 acggctcggg tgcaattaat tataatgctc tttacaacga attttttgaa ggacccgaac
    17461 agctgaatct gaatgtggaa ggtgaacttt ttggctggct gaaaaatctc actccctaca
    17521 ttttagaagc aaaaggaaga tcctctttaa gcataagcat tggcactagc gaagatcaat
    17581 ttctggtttc cagcggaaaa attaacatta acaatggatt tattcgcctt aaagaccaaa
    17641 cggaacccct tgccaacatc aatattagag gcggttttga taagaatcgc ctgattattg
    17701 aatatggaca ggttcaaatg ggagaaggca aactcatttt taataatatc tttgaagctg
    17761 ataacagcga ccattttatg ctgggattct tggatttggg aattttcaga gcgatgtgtg
    17821 aagaacctgg aattcttgta aacattccta aatatacaac tccccgttct ttaactaaag
    17881 taattttacg aggccaaaac agtcgttacg ccacaatccg aggtcccttt gaccaaatga
    17941 aaataagcgg agaagcagtt gcctccaaca ccaatgttct ttatcctcca gataccgaaa
    18001 atttacttac tatggcatct accgttcgtg gttccaccaa gaaaaaaacc tccgaaccca
    18061 accccctacc ttttatattg gatgttaaac tcattgttgg agagaatgtt aagtatgtaa
    18121 cctatcctgc agaactgaga gttgttccgg gtggatattt gcatttagct tatgatggtt
    18181 tatcattttt tgttaaagaa gcgtctttct cttcggaaca gggaactgtt gatattttcg
    18241 gcacaatctt tcaagtggaa aaagtaaatt taaccttagt tgactcccaa aatctgttag
    18301 gtttggaagg tattttttat aaacgagcac ctgatggttc tatgataact ctttcagcaa
    18361 taacatcttc cgattatact aagagtttta tggaacgctt gcaattaagc ttaacaagtg
    18421 ataaccctga agataaaacc atcagtcaaa ttttggctcg tttgcattat acgggaacag
    18481 aaactcccga cagaacacag ggtgaattct ggcaggatga agcattaggt ttaatttccg
    18541 gtaatcttga tgcttcgctt ttcactccca ttctttcacc catagaaaat tatattcgca
    18601 gaactctgca tttggatagc ttcaccataa atgctggttt tattcaaaat ctttatactg
    18661 aatataccaa taatcctcaa caatttgctg aatatacaga tatgaatcag cttagttccg
    18721 atatcgccaa attcagttct tctattttac tgaataactt atctatttca atgagtaaat
    18781 atctgggaag cagtattttt ctggactata tgctgaaatt acaagaggcc acaaacttgc
    18841 aaaaccgcac ccggattttg gtttccaatg aggcatcaat tcgtcttatc ttgcctaaaa
    18901 attaccgcgt agcttatact gttacctacg aagctcaaga aaaaaagacc agccatgaaa
    18961 ttatgctgtc aaggtttatt agattctggg gactgtgatg aatgaaaaac tgagaagaga
    19021 aaattgcgaa ttataaataa acctcggtgt tctcagtgtt ctcagtgtaa aaaagaatgg
    19081 aaaaatagga agtgaaatca gttcaattcg tgttgctatt aaatttatgc tatctgttag
    19141 ggacatttat aataaaatcc tttgacaaaa agcacaactt gaaaataaag gtaaaaatga
    19201 taaaaatccc gggaggatat aatgccggat aatgttaaaa acgaaaatgc agtgaatact
    19261 aaaaaaactc ctaaaatggc tgatttttcc accagagttt ccctggttga attgattatg
    19321 attttaatgc tggtgggctt ggtttttgtt ttttactttg gaatgaaaca actccaaata
    19381 gataaagcca atgaagcaat tgcacaggaa aaatttgaaa atataatccc cactttcaaa
    19441 aatatcattg aggcaatgga aacataccgt aaagctgatg aatttggaga ttatcctgcc
    19501 tttttggaag aactgaacct gggtgatatc aatacaaacg attttaaatt tgaatattca
    19561 gctgatactt atacaatcac agcaattact caacctgctt ttggaaaagc aggaattaag
    19621 gttatctaca atctttccga taaaagcttt acagttgaag accctatacc cgataaaaaa
    19681 cctaccataa aggatgaatg gttgccacaa gattaaaccc gccaataagt taacggggta
    19741 aatgagtgaa gtgaaagggt gaaacccgct ttataatatt gagggcaagg gtagaaaata
    19801 aaattttccg gagggtttat tcattcatcg ctgtggaagt attgttcttg ggaagttata
    19861 ctatgtcctt attcataaat cataaaggac agaaaaagtt ccgtaggaac gacagatatt
    19921 aacaacgggt ttcaacccgg tgataagaaa actgttatcc ctaaagtgac ttttattaaa
    19981 tgacctttca aaatgaaaat tatattacag gtattgggaa tagaaaagat aaatcccaaa
    20041 aatcctattc atcccaaacc ttttattatt aaatctaagt tatctgtgag aaaaaaacta
    20101 ttccataatt gctttcaaca acaacggcac cattatttca ttctgaccta tgaaataata
    20161 ccctttccct ccgttttcta cgggtctttc tgctacattt acctttgccc tgtagtgcac
    20221 attaaaatca aaaacggcag tagtaaaatt ctgaacctcg ccataaatat ttcgggcaac
    20281 cgttaatgcc ttcaaaaaca cctcgggaac aatcacagcc gagcccaaat ttaaaaacac
    20341 ccctccccca ttaagttcag aaaccttttc cgcaaaaatc cgaaaatccc gcattgaaca
    20401 atcaccaatt gcttttccat ccgcataagg gctttgatga ataatatctg ttcctatagc
    20461 aacctgcacg caaacaggaa tttcccattt ataggcagaa gcgagtaaag aaaggtcttt
    20521 gtattttgct tttgtataaa gttctttgcc aattgcctcg cctaaaccca aactctcttt
    20581 aaaaccctta tttattgctt cattaattcc ctttactgtt tcttccgcca tcccgaaaga
    20641 accatctgat aaagcagaag acacatcttc cgaggtctta ccgaaactgg caatttcata
    20701 atcatgaatc gctacggcac cgttacacac aaaagcggaa acaaaatcct cttccatcat
    20761 ttctattagc aaaggtgcca atccgcattt gataatatga gctcccaaac cgattattac
    20821 gggtttatgg ttttctctgg cttgtttgca ggcaagaata agctccttca ggtctttggc
    20881 agcaaggata tcgggaaggc aattgaaaaa atcattaacc ttcactttcc cctttttggc
    20941 aaaagcattt atatttactt tactatggcg ttggtcaatt ggataggtct ttattttctt
    21001 taggtctatc tctttgtatt tgctcacggt agcgttcctc ttcactaaat atacatccgc
    21061 aatattgttg acgataaagt ccggcttctt tagctaattt tctgccttcc caccataaag
    21121 atctccagtc ctcatagaag aagtcaatgc catatttggc gctcattttt aatgctatat
    21181 ccacaatgag ttcatgtttt tgatatttac tgtaaagcat tgtagtgctg aaagatgagt
    21241 aaccaagttc ttttgctgtt ttggcaacgg catttaaacg ggtttcatag caatattgac
    21301 agcggttatt aatatctttt agggttgtct gtaaaaaatt cattagaccg tattcatcct
    21361 gttcaagcag ttctatattc tcttttccgg caaaatcgcg caaagtatca cgcctttttt
    21421 gatattctaa aagcggatga atattgggat taaaccaaaa tgcagtcagt tctttccctt
    21481 cagctttcag cttataaaaa ggcgcaatca aacagggtgc gcaacagcaa tgcagtaaaa
    21541 gttctttttc tgccataatt ctatctcatt gttattcctg cgttcttgta atgccccaca
    21601 agcaggaatc cattcattta tcccccttca acatagatcc cgcattaagc gcggaatgat
    21661 aatcaccaat atctgcagaa aatcgtattt tgcctctctg ttaatactct ttcagtaaac
    21721 tggcagcaac tatgtttctc tgaatctgat tagtgccttc ataaatctgt aatatcttgg
    21781 catcgcgcat cattttttcc acaggatatt ctttcatata gccataaccg cctaaaattt
    21841 gaacagcatc ggttgtaact ctcatcgcta catcggaagc aaaatatttg cacatagcgc
    21901 tttctttgga ataatttctg gcaccactgt ctatcattct tgccgtttgc atcactaaag
    21961 cccttgctgc ctcaatttga gtagccatat ctgccagcat aaattggata ccttgaaagc
    22021 tggaaatcgg tttaccaaac tgctgtcttt ctttggaata ctttattgcc gcttcaaaag
    22081 ctccacgagc aactcctacg gattgagctc caaccatcgg acgggatttg tcaaaaacct
    22141 tcatcgcagt aataaaaccg gtgccttcac gcccaatcaa attccctgcc ggaactttac
    22201 aatcctcaaa aataagctct cttgtagcgg atgcgcgaat acccattttg ttttcttttt
    22261 ttccaaaatt gaagccagga gtgccttttt ccacgagtaa acaggaacaa ccacgcgcac
    22321 ctttagtttt atccgtcatc gcaaaaacac aataaattcc tgcttctccg ccatttgtaa
    22381 tccattgttt tgtgccattt aaaatataat aatcgccttc ttttttagca gtggtctcaa
    22441 ttgcaccggc atcactgcct gcatttgcct ctgttaaagc aaaagcagca agttgttctc
    22501 cagctgcaat ttgtgccaaa tatttctgct tttgctcttc agttcccgca atcaaaatag
    22561 gatacattcc caaacctgtt gctccaaaag ctaaagaaat acctacatca acagcacaga
    22621 gctcttctgt aactactgcc aaatcagcaa ctttaccgct aattccacca tattcttccg
    22681 gaactaaaat agcaaaaaga tcgctttgtt tcatcacttc tacgatgtcc caggggaaaa
    22741 tgccctcttc atcgtatttt tcggaaacag gtctaatttt ttcctctgca atccgccgtg
    22801 ctatttcctg catttccaat tgttcttcag ttaaaaagta gtccattatg taactcctat
    22861 attattttat gggttataca ttttagcatt aggcaaaaat tccctaaaac tgtcaatcta
    22921 ttttttcctt ccttaattac agctcatcat ccacccatct tcaacccatc gtaaaggttg
    22981 ggttgaggat gagtgaattg taggcaagct ataggtactt tgcggaaatg aaaaaatgag
    23041 agtcaggaac cgtcactgcc gaaataactt atatactacc cacgaattta tttggcagtg
    23101 aggactcgtg acgaccagaa tgaaaaatga aaaaattgag aattatcaat aaatctcggt
    23161 gtcctcagtg ctctcggtgt aaaaatatct cttttctctt tatctctttt tctctttgtg
    23221 aaataattct ctcggtgctc tcggtgtaaa aaaaatctat attttgtttt tactccccaa
    23281 acccaaaata taaaattcta tatttggatt ttacctccta aaaccaaaat ataaaattcc
    23341 atatcttgct ttattcctta aaaacatcaa tatatctttt aaacataaac accctatttc
    23401 tgctgtatcc tgttgtttcc tctaaaatat ttatctctac aagggttttt attaattcat
    23461 tggcagagta gtatttaacg cccaaaattg attccacttg cttaacacta acaacaggtg
    23521 actgatataa gtgcatcaat aattttctgg cgttatcggt ttttttacca agattcataa
    23581 gttcactttc aatattggta tgtaatttta gaatttgggc aaaagtcgtt tttccttttt
    23641 ccgctgtttc aataacccct tccagaaaaa aacgcaccca ctgtcctaaa tcattggagt
    23701 tccttactct ggttagcgaa tcgtaataag ctccccgatg tctttcaaaa aaatctgaaa
    23761 gatataaagt cggtttaatt aaaagatgat tactaaccag atacaatgtt atcagtaaac
    23821 gtccaatacg tccatttcca tcttgaaaag gatgtatggt ttcaaactga tagtgagcta
    23881 tagcacaacg aattaaatga ggaacaacaa ttctgtcatt ataaagaaaa tgttcaaaat
    23941 catcaagtaa atcagacata tcatctttat taggaggtat aaaagctgca tcagctaaag
    24001 atgagccacc aatccaattc tgacttgtgc gaaactcacc cggggtttta tgttttcctc
    24061 ggacattagt catcaatatc cgatgtgtat ttctaattaa acgcaaagaa agtggtaagt
    24121 tttctaactg ttttatggct tcgttcattg cctgaacata attttgcact tcctgccaat
    24181 catcccgttt ttcaggataa actgaactct catctaagac cacatcttca atatcggttt
    24241 tagtgccttc aatccgactg gaagtatttg cttctttcaa aatatgcatc cgaataaata
    24301 agtccacatt aggaacaatt cgggaaaagg catttaattc acccaatgtc tgtgctgctt
    24361 tttccagtaa aagattaatc ctgggatcat cccaagtcca ttgatggttt attttacttg
    24421 gtaaaaaact cttatattga aattgatttt tataagttcc ggatttgaat tcttcaaatt
    24481 tcatcgcatt aaacctgcct gtttctttta gttaattact ctttccttcc tataattagg
    24541 catatttaag tcaagaactt tatgcgatgg caaaaaagat ttattttttg caccgaaatc
    24601 agaatcaata atgaaaaagg aagaaatgtg aggcaggagc cgtcactgta gaaataactt
    24661 atatactacc cacgaattta tttggcagtg aggactcgtg acgaccagaa tgaaaaatga
    24721 aaaaattgag aattgtcaat aaatctcggt gtcctcagtg ctcttggtgt aaaaatatct
    24781 cttttctctt tccctctttg taaaattctc ctcttttctc ttttctcttt ccctcttttt
    24841 ctctttgtaa aataattctc tcggtgctct cggtgaaata aaatctgcct accttctcta
    24901 ttttactatt atcttccctt tcgcctttcc actgtggtaa aaatataacc cgcttcccat
    24961 tccttttccc ttaaggtcta ttcttgcttc tcccttttgc acaggcactt gtaacacttt
    25021 ttgtcccttt agattataaa tctttagttc tccataaaaa gcattatccg ccttgaaatt
    25081 gagaatacca tctttattaa ccggattagg atagagagtt atttgaggca aaggcaattc
    25141 ttcatcggaa acaggggttt cattgctata ataacaaatg tcagttggga ttaatgcaac
    25201 cgtccactga gtaatcggtt gccaattatt attccttaaa tatacagttc cattttgact
    25261 atagggagca taaagaacag caatacaatc agttgaaccg ctgatcatta tccctccagg
    25321 agttaaagga ttactgctgt tatgcaaaat ttccagggtt gaagcattat aagaatatac
    25381 tccgctatta tatccctctc ccaaataggc aaaaccttgt gggctaatcc atacactcca
    25441 agggttgcct ccaattaaaa gacggtctat gcgttcatcg gttacggaat caataatatc
    25501 caccgctcca gaaatggaac tccaattacc ggtgcatact atatgcaatt gtgaaccgaa
    25561 aacagctata tcctgagggt tagtccacac aggaatggtt tttattacgc taaaagaatc
    25621 caaatcaatc acagagacac tgctattagc ataaccgcta gcataaccac ctgtattaca
    25681 tacatataat tttccgttac agacctctaa accttccggt gctgtgccaa ctgtaatttg
    25741 atctataact gtccagcttt cttgactgat tttatagacc ttattagtaa acattccgct
    25801 tacataaaga taaccattat cttctactac atcccaggga ttggaactgg aggcaataaa
    25861 aatattacgg atagttgttc cactatttct atctatcatc tgaatgctgt ttgctccgga
    25921 aagaactaca taaataaaat cactattaac tttcagcata ttcggagtta aacccaattg
    25981 cgtaaaacta ttattcaccg ttcctgtttc ggtgtcaatt ctgcttaaag ttcgggattc
    26041 ggaatttacc acataaatat aatccgcctg cagaaacgca atacagatta ctggaagcag
    26101 aagaagtaaa aagaactttt tcatcatatc ctcatttagt taaaaataac aggtcttctc
    26161 gaaagaatac ggtaaccttg gagccattat taaaaaatcc tcttttgtgt caaatcccaa
    26221 tatgcttctg ggcaatttta cttttcaaca gtaattataa tttgcttccg taacaaaatg
    26281 ccaatagata tatgatatta gcagatagaa gtgattattt tgggcaaggc aagggaaaaa
    26341 atggaactta aggacaaaat aatacccgta gatgaactga tacccgtaat tgaagatatt
    26401 ggagccataa gcttttataa cattgtgaaa gattatattt caggtaacta taatacctca
    26461 gcatttgagc cagatgatga tcttgattgt cgtgatcctt ttctccaaca agcttatgaa
    26521 cataatctgc tttcagatag ccaaattaaa gaaatatacg aaattgatga atttgtttct
    26581 ctcactcggt tccccggaat cataagaatt taaagagcgg cttaaacccc attcttttgc
    26641 taccaataaa actacaaaac gaaatgagtt ttatagtaac gacagatact aacaacgggt
    26701 ttcaacccgg tgataaggaa agaacctata aactatattt attggggaaa aaagttccgt
    26761 aggaacgaca gatactaaca acgggtttca acccggtgaa aggataatag gataaagctt
    26821 atctcttttt ttgagaataa gttccagagg aacggcagat actaacaacg ggtttcaacc
    26881 cggtgataag aaagcagtaa agtcattatc tcttttccga gaaaaagttc cgtaggaacg
    26941 acagatacta acaacgggtt tcaacccggt gataagaaag caggagagtc attatctcct
    27001 tttccgagaa agagttccgg aggaacgaca gatgttagcc caagtttcgc aattatcaag
    27061 ctaaagtatt gttaaataag acaaattatt ttttttgcac actacaatct atatttttgt
    27121 gataccgtat ttttggaaac cttgatattc aaaagggaat agattttctt cgcttttgcc
    27181 tcaggttggg ctgtttgttt gtttctaact ctataaacat ctgtagtatt gaatattgta
    27241 tccgaacaca tataggtttg caggatttgt ttgatgcttt gccagctaat atgaacatct
    27301 tcaagagcca gcttataggt tatacttttc agaatagcgt acgctaaaac ggaatagaac
    27361 aaatgtactt ctatccgacc atctttctga tgataaatag gtcttgttcc cagttctgtt
    27421 ttcatacttc ggaaggcagc ttccacttca ctcaaattca tataaaattt ccagatttct
    27481 tcagcactga aatctgtata gttggtttca attacataac aacccggaaa ctcagctttg
    27541 caggatttct gataccgatc agttattatg agagattcta tcctctgagg agcagaattg
    27601 cttggctgg
//
LOCUS       DAOOZV010000021        27478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1906_length_27478_cov_63.276046, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000021 DAOOZV010000000
VERSION     DAOOZV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27478
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_27478_cov_63.276046"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1009)
                     /gene="ftsY"
                     /locus_tag="PLW08_03555"
     CDS             complement(44..1009)
                     /gene="ftsY"
                     /locus_tag="PLW08_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPL51710.1"
                     /translation="MSTIIIKNGDKKVLNIVKNLKEKLAKTNSNFMGKITETVKKRKV
                     VDEKMLAEIEEILLSCDTGIEMTDIILTRFKEQLKKDKVTDPEIAQIYLTDVMRDILL
                     AENEETPDFFAEPQVKPYVVVFVGVNGTGKTTTIGKVAYKFNQMGKKVLIVAGDTFRA
                     AAIEQIAIWAERAGVPIVRSQPDSDPAAIIYDGVHSALARGYDVVLIDTAGRQHTKDN
                     LMKELSKIERTIQKLIPDAPHEVILVVDATTGQNAVSQAHNFDKAMKLTGIALTKYDG
                     TSKGGIIFNLKYNLQLPVKLLGVGEGIEDLLPFKAVPFVKAFFEE"
     gene            1114..1734
                     /locus_tag="PLW08_03560"
     CDS             1114..1734
                     /locus_tag="PLW08_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51711.1"
                     /translation="MERTKILPRDLLLLFFPIIAAILGGLLFSGSFTEFTANLSGKDI
                     SQAELYFALSFYIGALAFGYSCLPKNVLLGLQIFIPLLYGLLMLLRFKVELSLFLLFI
                     LNLVCGFVLWLILRFTYFSKTLITMRTVIFSVASALVLSVYFKLLMSLLKQAKGANTF
                     MDYFLNALVLFIFIGVGISLAILIITRKEIKEKSKNPKEDEEDDDF"
     gene            1724..2350
                     /gene="trmB"
                     /locus_tag="PLW08_03565"
     CDS             1724..2350
                     /gene="trmB"
                     /locus_tag="PLW08_03565"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPL51712.1"
                     /translation="MISDRDFFVIKPQINEQGKFISLNPVDLFHNSHPLYLEIGCGKG
                     EFIARYSILHPEYNYIGLEAAEKRINNTLKKLTPEQNPNVRLMRLYVDNSICQLFPAE
                     SVSGVFIQYPDPWPKRKHHRRRLIQQEFLTSLAMILKPLAQVQITTDNSDYAAWILDE
                     FLAHPSFISVYEELMLKESPFEEHIPTWYESEQRRQGFEPQYMLFKRI"
     gene            2363..4054
                     /locus_tag="PLW08_03570"
     CDS             2363..4054
                     /locus_tag="PLW08_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPL51713.1"
                     /translation="MSYNFSSNYQINKMLNSVVESITNLAEEQLLHIQRLTRIGESLS
                     SETDIDKIFDMILEEGISFTRADAATIYTVNEDATKLQFEIVYNATLNLRKGGTRGPV
                     NWKSIDLYDEKGNPVDNKIVTNVYHHKQSLCFPDVYKAEGYDVSGTIKTDRENNYRCK
                     SMLTIPLKNHEDTVLGVIQFINAMDEEGNIISFTDEHKAMLSSLASQAAIALSNKKLI
                     ENLEELLNQFIRSIAGAIERKSKYSSNHITRVAMLTEMIATKINEKGEKYFPGRKFNE
                     NELKELSMAGWMHDVGKIVTPEAIMDKSTKLETICDRIELVKLRLEKLELLLTYLQLK
                     LKEDEFAAFIHQNIDSELEPTGFRNYLANITNFLINVNVGKEFVDKADLEKVEKLGKI
                     NFEYEGKRYFIFTEDEKKNMQISGRGTFTPEEMKIMQDHVAITWEMLSQLSFPKKYEN
                     VAFYAATHHEALNGKGYPNGYTADKLPLQSRILAVADIFEALTASDRPYKTAKTLSES
                     LNIMGYMVKDGHLDKDLVNFFLDSGLYKEYADRFMNKEYIDEVDIDSIKAIYTNQ"
     gene            4051..5016
                     /gene="dusB"
                     /locus_tag="PLW08_03575"
     CDS             4051..5016
                     /gene="dusB"
                     /locus_tag="PLW08_03575"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPL51714.1"
                     /translation="MTVPFLNKPLLFLAPLAGYSDSAFRQICKNWGAEVLYSEMVSAD
                     GITRNSAKTLQYVQFDESEHPFGIQIFGSNPLIMAKGVEVLLPLKPDFIDINMGCPVK
                     KVVRKGAGSALMQTPSLAGKIVKEVKNALLGRIPLSVKFRSGWNFQNQNYLQFGLMLQ
                     ECGTDFLCLHPRTSSQMFSGSSNWEQIKILKQNVQIPVVGNGDIKTPEDAQRMITETN
                     CDGIMIGRGALGKPWLFAQSRQLISTGTYNPVTQKEIVKTIFAHLDKALLYKSEDVVV
                     REIRAHLCFYTKGILGGAELRNQINHTENISELKAIILKGFSHIG"
     gene            5367..5972
                     /locus_tag="PLW08_03580"
     CDS             5367..5972
                     /locus_tag="PLW08_03580"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPL51715.1"
                     /translation="MKYFRKLVGEKCYLSPVSMDDVEKYTEWLNDMEIGQFVTLSHIV
                     LDIEKETELLRKLMREDYIFAIVEKDTNKVIGNCGIHQVSQVHRNASLGIFIGEKTFW
                     NQGIGTEATSLLLDFAFNILNLHNVYLSVMSYNKRAIRCYEKIGFKKVGVQREFMFVS
                     GQYHDVFIYDMLASEFTSPYVKKIYTYSISDEAGRSKISIV"
     gene            5973..7085
                     /locus_tag="PLW08_03585"
     CDS             5973..7085
                     /locus_tag="PLW08_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPL51716.1"
                     /translation="MLPKIKGQENALELLDKAIENDRIAQAYLFYGSDGVGKFTTALY
                     FGMAVNCLATSELKPCGECDACHKFLQLEHPDFVYLFPTPNLNLSAEGEIKNTDAQKS
                     YEAYIQNKINTPWKDFFFKEATEIRKESITLLNKRLELSIHEAKYRIVIIEDADMMNN
                     ATANAFLKTLEEPPERTIIILITERLPLILPTILSRTQPVYFKPLTRGVIEEILINEF
                     EINSALARSASRICAGNLKTAIRIASESESLSSNWAFEIFSLAAEENDLGYLDLAERY
                     KKQQTIDQVIDLLKYIRIIASDLGSLSANPDSEITNIDKIDFLKPIAEANLGIDDRLY
                     DFLLFLEDLNRKVEGYANLNLVLTNLYLNCKHLLKP"
     gene            7086..8660
                     /gene="murJ"
                     /locus_tag="PLW08_03590"
     CDS             7086..8660
                     /gene="murJ"
                     /locus_tag="PLW08_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPL51717.1"
                     /translation="MEAKGTNRTLAKNISVMSIGVFISRIFGLIRDQVMAYFFGTTYW
                     NDAFNVGYNIPNLLRRLFGEGALSTAFVPLYNDIKIKQGKEKQIEFALNLLSVLTFIL
                     CILTILGIALAPLIVKCLYPGLASETKVLAIKLTRIIFPYLFFIGLSSTFIAILNSHN
                     YFFMTGLSSALLNIGMIATVLIPHFVLKVSGEDLIVWAGGGVLVGGFLQTVINLPYLK
                     RIGYRWAIYLKFGSEALSVLWKRFIPSMIGIGIREINLIADSLMASFLPTGSITALNF
                     GNRLMQLPLGIFAISTGTAVLPFYSRCVSTENYQELSESIRFSGLNIAYIMLPVTTII
                     LALGEDFVRILFENGAFQGDAVLMTSQALIFYSLGLIFYGLNQTITPVFYAYKDTKTP
                     VKIAAGMVALNITLNFILIQFMAHRGLALSTSITACVNFFILLFLIRKKMPEISFSGI
                     AINIIKSLLICCFLYLLLYISCNLFPVSGRISLLIRDAILSIIALGLFYLTGILLKID
                     YLNQAGKKLCQRFLKK"
     gene            8636..10441
                     /gene="uvrC"
                     /locus_tag="PLW08_03595"
     CDS             8636..10441
                     /gene="uvrC"
                     /locus_tag="PLW08_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPL51718.1"
                     /translation="MPAFSEEIETKLALLPEQPGVYLWKDKDGTIIYIGKALNLKHRI
                     KNYLNPTGKDLKTEHLIKNIADLDYIITNSEADAFILEATLVKRYRPKYNIMLKDDKR
                     YPFVKITVNEPFPRIFITRELLKDNAKYFGPYTEVRYLRNTLRDLEWIFPLRSCNRNI
                     PKIKYKQPCMNFQLGKCPAPCIGNISQKDYAKTVNQLLRFFNGRYTELLDELRTEMQN
                     ASSELRFEEAGKFRDRIIAIERIQKRQSVFNVEGGNIDIIGFYREEKRAVCVILKMKN
                     GAIIHQENYPLTNLDYEEPDSILASFLQLYYADREEMPEEVLLPFAPKDMDKLNSWLG
                     NKLQIPQRGEKTKLIAMAKTNAFNIVEESKLAHLRKANRTIFPVQELKEALKLPKLPR
                     KIVCMDISTIQGSDTVSSAVFFENGKSKKKYYRHFIIRSIETQNDYAAMQETMARFLT
                     ETQKEPQMKPDLIVIDGGKGQLSASEQILRASGNNDISIIALAKRAEEVFVPGNEQSI
                     ILPRSSSALRLLINIRDEAHRFAISLHRKRRSRRTLESELESISGIGEKNKFLLLKEL
                     GSVENIANANKETLSKIKGIGPKIAEKIYNHFHST"
     assembly_gap    10683..10782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11141..11581
                     /locus_tag="PLW08_03600"
     CDS             11141..11581
                     /locus_tag="PLW08_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HPL51719.1"
                     /translation="MSGEFLGIFENAVHKQRVIIPAAFKKKFSEEAEKKVVVTLGPNN
                     TIAIYPLDCWKATLERLKNGDEHSHKLRTQLIDFAMTEQELEGPGRVRIHEMLLNEVN
                     ITDSVVIKGEGHYISLWNPKVYNEVRASKLDQHRKQFTSEDYQL"
     gene            11578..12561
                     /gene="rsmH"
                     /locus_tag="PLW08_03605"
     CDS             11578..12561
                     /gene="rsmH"
                     /locus_tag="PLW08_03605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPL51720.1"
                     /translation="MTFHTPVMVKECLSYLNLRAGHIYVDATTGGGGHSLAMLKAQPE
                     IKLFCFDQDSEAIEEAGKILHNYNNVELIQANFKQLRTELAYRKIKGIDGILFDLGVS
                     SHQLECAERGFSFEKDAPLDMRMDKNLKNSAYNAVNELDAKTLTNIFREYGEEQNAYR
                     IAKAIEKSSKPIKTTVELAKIVESVAGKGTKASLKTKVRVFQALRIYVNKELESLSLA
                     LQDAINLLNPQGRIVVISYHSLEDRIVKQTFKKAEQKNEEQSVLPNSNKNHQQQLIIL
                     TKKPVTATEEEILVNPRSRSAKLRAGEKVQREKRETKTSRKPNRQNSQEEK"
     gene            12558..12914
                     /locus_tag="PLW08_03610"
     CDS             12558..12914
                     /locus_tag="PLW08_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51721.1"
                     /translation="MRGRILILVSLFAIVFFLNFYNNNRIVNYTRQISALEKTYYAEK
                     NINTELLVELDDLRSGRHIASLVRVELSNFIPEKQAGKIIYVHEPSPKQEKKTYCIID
                     LLSKKAEAKNVQILLD"
     gene            12892..15090
                     /locus_tag="PLW08_03615"
     CDS             12892..15090
                     /locus_tag="PLW08_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPL51722.1"
                     /translation="MCKFCWTKMKSRYYFLVVIFALVAVIWIAYLFCIQILDPFHLAG
                     ARRMRYTPQKEILIPRRGSILDAQGNLLVSSVIYYQIDIDRSSVASWAKEEKIPLEKA
                     FEKYAEVISSHTTLNKDDVIKRLNYGNKTSSIQISNKISEVELDKLIKELKTKDMPGL
                     VHSFASMKRIYSKDILAARVLGSVREVSDGYDPATNSKSLYKLAGVCGIESTYDKYLS
                     GNYGWKEVVLDANHQPVPYPNLHSKKPENGYNLYLTIDSKIQEIVENALYEGMEHYEA
                     KNAGAIVMDPNTGKILAMAGVSGEDKTEDPNFVRVKSNIPVSFMFEPGSTMKPLTMLP
                     AIEHKLVKPTETFQCGRYQVGRRVISDTHQYGELTPREIITKSSNVGIAKIAERIGPT
                     RLYENFIALGFGQKTALNLAGESSGMFAKLQNWDGYTLHSIAFGQGISTTALQLATAY
                     SAIANGGKLMKPIIVETIKDDEGKIIEQFEPSVLRNVASTAATDTIKSYLQGVVDSGT
                     ARHIKMDYIQVGGKTGTAQKNITGTKGYSQGKYSSVFIGMFPIEKPQMVVVVFFDEPA
                     PGFHYGSTSSAPTFKKIVENILFMPDCQILPYNERLMQTSLTMPKLTGMHLFQAENTL
                     SRYGFQYKVEGPDSASIVIDQYPKAGVTVDPHYPITLKIGPNTDKKAPVYETGTMPNL
                     VGLSLREALKQASIQGLAVNIRGSGMVRSQSVLPGSRILKGSKCYLEASL"
     gene            15087..17936
                     /locus_tag="PLW08_03620"
     CDS             15087..17936
                     /locus_tag="PLW08_03620"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPL51723.1"
                     /translation="MINRILYALQQHSLLVETANLAKLENWQGKVQTDNRKIKPGDIF
                     VCIQGANFDGHNFINSALEKGADIIVCREINKRDIPYIKVKDTRKAAAVIARTVMLPE
                     KIPYTLIGITGTNGKTTTSSLLYEALRNLGFRCGWIGTLGYYIEGKAFPSERTTPDII
                     ELNEIFGQMIEAKVDYVVMEVSSHSLSLDRIYGVKFDYCLFSNLGRDHLDFHQTLENY
                     GKAKMSFFEQGAKNGSISVINTADAFGVQIKDMLKEKQAKVYSLGGNDAEFQYSDIKT
                     DINTSSFKLIAENCEIPVTSRLIGSFNIQNLGLTALTLYALGFSPEAIHNSLKDIPPV
                     QGRLERVENNFNIGIFIDYAHTPEAIDNVLKAVEDLPHKRILCLIGAGGDRDKGKRPL
                     MLKSALNHSDVVIISDDNPRSENPERIIKDIVSSSDIFLPWWIIRNRHQAISSIIRLA
                     QEEDIVLICGKGSEDYQEIEGVRHHFNDREEALKALAEKEIKDKADDELILPIDQTLL
                     KLLLLPNWEPEPKGYQETYSYYYLSIDSRSIKPGSVFIAIKGEKYDGHSYLEDVLKIK
                     ENCAIGEIPYPLNQFSEENSYYQVENCLETMGLICRKYLQLFPAKRIALTGSTGKTTC
                     KELMAQVLQSSAPVLKTLANENNIIGLCKTIIRIKPEQQYAIFELGTNHFGEIAALAD
                     VANPDIGIILNIGPSHLEYFGDEEGVFREKITLFKRPLALRLYPGDDFRFDYLANEGI
                     SIGYNENCDYRITRHKCNKDNQSFYLNEDLWFLPYGAPHYAINSAFAIVLGLKLGIDK
                     EKMQTALNKPIQLELRSHVEKRGTGLLILDCYNANPYSMQKALEYWQELNPEQPHYAI
                     LGDMLELGESSILYHQMISAMLAEMRYEKLFTVGNYSRIYHPEKESPILHYNTVEELI
                     ADRKLNSLPENAIILVKGSHAIQLERVIPILKGED"
     gene            17936..19039
                     /gene="mraY"
                     /locus_tag="PLW08_03625"
     CDS             17936..19039
                     /gene="mraY"
                     /locus_tag="PLW08_03625"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPL51724.1"
                     /translation="MFYHLLYPLAKYSIVFNVFRYVTFRSIGAFITSLIISLLVGPAF
                     IRMLKKKSAVETIDEDLPERHRLKQGTPTMGGIIILVSLLISSLLWNVLTNPAILMII
                     LTTVWLGILGFLDDYLKNYVKAKKGLIPRYKLLGQISVGLILTLAIYYSSSVSENITA
                     MQIPFFKNLIVPLGWVFIPFAVLFITGTSNAVNLTDGLDGLAAGTLIFSFLGLGVMSY
                     LKGNYIAANYLNLEFISSAGELTVFISAIMGTLMGFLWFNSYPAEVFMGDTGSLTLGG
                     IAAVISILLKEEIFFFILGFIFVYEALSVIIQRSWFKYTKRKYGEGHRVFLCAPVHHH
                     YELKGIHESKIVMRFYIVAALLTAVALSTIKLR"
     gene            19053..20405
                     /gene="murD"
                     /locus_tag="PLW08_03630"
     CDS             19053..20405
                     /gene="murD"
                     /locus_tag="PLW08_03630"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPL51725.1"
                     /translation="MIDKDKKYGIIGLARSGIASAYKLKELGVIPFLSELRPAKEIPT
                     AEKLEKDFPCEFGGHTNKLLECDCLIVSPGIPLNTPILQKAKEKNIELISEIELGYRI
                     KAKDSKIIAVTGSNGKSTTVSLIHHILTGLGKKSLLAGNIGDAFCSYDIEKPGWDYIV
                     LEISSFQLDLINSFQPDVAVLLNITPDHIDRYNSFEDYALSKVNIFKNQEMTDTAVLF
                     WDSPEVKHFTKDIKSRKLYFSLSPNDNAFAYLTNNYIQFGSETKVPLSYFAIKGPHNY
                     ANAMSAMLAVYALIPEPTKIAEAVRGFKSLPHRLEYVDTIKGVSFYNDSKATNTDSVR
                     SALNSFEQPIRIIMGGSDKGEDYSVLAADLQKKVKKAYLTGDTQEKMRKTFSGKIPLE
                     CIADFELTVRKAFSDSGEGDIIVLSPACASFDRFNNYEERGNFFKEIVARIGKENEKK
                     "
     gene            20392..21531
                     /locus_tag="PLW08_03635"
     CDS             20392..21531
                     /locus_tag="PLW08_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPL51726.1"
                     /translation="MRRNKTPSYIVSFDKVILITYCLLCLVGLITLLDISSVQSSMKY
                     FYRQLVFLIISMITVIVILYTFNLEKLRVLSPYFVYLTIILLIIVLLKGSTVKGATRQ
                     LSLGFINFQPSVLARLALVFYFAHILDKKYDELVASNPLHFFTNFFALIVITGITFLL
                     IIMERHLSTLIIGGLTLYAMLIYAGTKKRVLISLALIGIIAGVLILANGADYRKGRLT
                     TYKKFSLFLRPEGEIKIEDSDYQVKESLTALSSGGLIGTGMAKGRAKHYYLPEARTDY
                     VYTVIGEEWGFIGALIVFGLHCFLFFRCFRMANSQENRFLRFLGVGLAMNIFCNVLVN
                     TGVAMSILPPTGNTLPFISYGGSALLIDSIALGMLLNISAQRRYV"
     gene            21528..22616
                     /gene="murG"
                     /locus_tag="PLW08_03640"
     CDS             21528..22616
                     /gene="murG"
                     /locus_tag="PLW08_03640"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPL51727.1"
                     /translation="MKFIFGAGGTGGHITPALALADELVRYKHNVLFLGNRQSIEETL
                     CASAGYPFRQIKVQKLHRSLKPENMLVPFYLLGSVITAVNIMKKEKPQAVICTGGFVA
                     GPVAISAALLKIPLFFHESNSYPGLVTRKMAKWINTIFISFENSRKYLSRAKLVNYGI
                     PLRQITNKDQKPFELSSLGLKNDKPTLIVSGGSQGSVAINTVVNQAISDILSLGYQII
                     WQTGKISYERFSAKHKGREGVYIFAFSPDLPNMLSQCSLAITRAGAMTIAELEENRLP
                     AILIPLPTAAENHQYYNALAQQEKGVAVLLEQSKLTPFSLVENIKKITENLEYYRNKL
                     ASLPPNQAAQKIIEYILNYLNSKEEEEC"
     gene            22610..24004
                     /gene="murC"
                     /locus_tag="PLW08_03645"
     CDS             22610..24004
                     /gene="murC"
                     /locus_tag="PLW08_03645"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPL51728.1"
                     /translation="MLGRTRKIHFVGIGGIGMSGIAEFLHNQGLEITGSDLKKSDITT
                     HLESLGIKIFEGHNPENIGDADVVVKSSAINDDNPEIISANEMKIPVIRRAELLAEIT
                     RMSFSIGIAGTHGKTTSTSMAGSVLESAGLEPTIIVGGKVKNFGSNNVMGSGKYIVVE
                     ADEYDHSFLTLTPCIAGITNVDADHLDCYRNLDEIKSSFIEYANKVPFFGSVIACLDD
                     PGVQAILPFIHKKIVTYGFSRQADVQARDIDMQGFNSSYDLLFKDYKLGRISLKVTGK
                     HNIQNSLLAASIGLELDIPFPAIQDGLGKFSGVYRRFDLKGEAGGITVYDDYAHHPTE
                     IIATLEGFKDSAKRRIVALFQPHLYSRTRDFYEEFGKSFFSCDCLILAPIYPAREQPI
                     PGISSKLIADAAIQSGHHNVHLINSNSEIVSKTLALLKEGDILITMGAGNIWQYGEEI
                     LRQLKNYVDTNHKQ"
     gene            24085..24852
                     /locus_tag="PLW08_03650"
     CDS             24085..24852
                     /locus_tag="PLW08_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPL51729.1"
                     /translation="MELNKGTRKRRGNSRYYLFFFFALIGVALLGTGIWFGLTHIDLF
                     TLQKVTVIGNEAIPDTLIYKITQPYIGMNLLAIPAEDIKNKVMNLSRVKDVKLHKRLP
                     STIKLEINERKAAIYLKTIEGDLHPIDSEAVVLMKYSPIYKEDMPIYSTYLSNHQIKP
                     GHKLKNAGLQQVLQLHKRITKEAPDFLPQISEYYLIDKTVNIIDAKTGTRIIPAQEDL
                     AKQLARYQFVQENGNINKNSVVDLRYKNQVVVKAGNK"
     gene            24849..26120
                     /gene="ftsA"
                     /locus_tag="PLW08_03655"
     CDS             24849..26120
                     /gene="ftsA"
                     /locus_tag="PLW08_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPL51730.1"
                     /translation="MKQSIITALDIGSSNVRCIIAKEVGDGRLEILGLGEYPSEGIEG
                     GIVKDIQALSGCVAKAISTAEKEGKTSANNIYCNITGEHIRTQFGDGRISIPSETPNE
                     PGEITQEHIEQVINDAKNSVKIQKGLERFRILHGIPHNFVIDNQDDIHNPVNMNGFHL
                     IAKVYTILAELTPLRNLSKCIQLAGYEINPENFILNHIAISESVLSEDERRLGALVLD
                     IGGGTCDLSIYNRGSLEKILVLPMAGKNITEDLAIGLKTTLGNAEYIKVEYGNALASS
                     VDQTEEIDVEGISGRSTSRKTKFLVSHVIQHRVEEMLSLCYNKAKEFYTPELVTSGII
                     LCGGTAKLKNIEVVLSEAFNLHVKIATPDLSRLNGMISRLEDPAYATVVGILYFAAGK
                     NNEPVSKGFTLPNIKGKKIVDKVKKILKDFT"
     gene            26133..27317
                     /gene="ftsZ"
                     /locus_tag="PLW08_03660"
     CDS             26133..27317
                     /gene="ftsZ"
                     /locus_tag="PLW08_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPL51731.1"
                     /translation="MIEFDEKPAQIGTNIKIIGVGGAGGNALNTMIENNLFGVEFIAA
                     NTDIRDLTKSKANMKLQLGKKLTRGLGTGANPELGARSAEESKEDIKNHLDGADMVFI
                     AAGMGGGTGTGASPIIAKIAREMGILTFGIVTSPFPYEGKKRAENAIYGIKHLREFVD
                     TLLVIPNEKLCEIYANLTLKEAFKKAEFVLYEAARAVSDIINVTGLINVDFADVKAIM
                     QNMGYALIGSGIAEGENRAINAARAAIDNPLLSHISLQGCQSLLLNITAGYDILMSEF
                     DEVSNVIVSETGKAANIIMGIILDDAMAGKIQVTIIATGLEKEDKEHTIDFPGLPEFG
                     EKSVPSITQSEDTNSEIEDIFARLSINQSTPKEEVKPAANEPTRIPPLKTDVPSFLKA
                     LD"
BASE COUNT         8789 a         4825 c         5428 g         8336 t
ORIGIN      
        1 tcctccggct acacattctc tttttctcta tgtgaaaaaa gctttattct tcaaagaacg
       61 cttttacaaa cggtacagct ttaaacggca gcaaatcctc aatcccttct ccaacaccta
      121 aaagtttaac cggtaattgc aaattatatt tcaggttaaa aataatacct cctttggaag
      181 ttccgtcata tttagtgaga gcaattccgg ttaatttcat tgctttatca aaattatgag
      241 cttgggaaac ggcattttgt cccgttgtag catctacaac taaaatcact tcgtgaggtg
      301 catcaggaat aagtttctgg atagtgcgtt caattttgct cagttctttc atcaaattat
      361 ctttggtatg ttgtctgcca gcagtatcaa ttaaaacaac atcatagccc cgagccagag
      421 ctgaatgaac accatcataa ataatagcgg caggatcgct atccggttga gaacgaacaa
      481 ttggcacacc tgctctttca gcccaaatgg ctatttgttc aatagcggca gcacggaaag
      541 tatctccagc aacgattaaa accttttttc ccatttggtt aaatttatag gcaactttgc
      601 cgatggtagt ggttttgcca gtgccgttta cacctacgaa gacaaccaca taaggtttta
      661 cttgcggttc ggcaaaaaaa tcgggtgttt cttcattttc cgccagcagg atatctctca
      721 ttacatcggt aagataaatc tgagcaattt ccgggtcggt aactttatcc tttttcagct
      781 gttctttaaa gcgggttaaa ataatatccg tcatttctat tccggtatca cagctaagca
      841 gaatttcttc aatttccgca agcatttttt catcaaccac tttgcgtttt ttaacggtct
      901 cagttatttt tcccataaaa ttgctattgg ttttagcgag tttctctttc aggtttttaa
      961 cgatatttag cactttttta tctccgtttt taattattat tgttgacatt ataagcaact
     1021 gcgttaaatt tgtatttagg ggctggaagt caatataaat ttgtttcagt cctgttctga
     1081 atgcagttca gaaaacaaca atgaaggttt tgcatggaac gcacaaagat attacccagg
     1141 gatttattac tgctcttttt tccgataata gcagcaatct taggggggtt gcttttttcg
     1201 ggaagtttta ctgaatttac tgctaatttg agtggtaaag acatcagtca ggcagagtta
     1261 tattttgccc tcagttttta tattggagca ttagcttttg gatacagctg tcttcctaaa
     1321 aatgtattat tgggtttaca aatttttata ccgcttttat acggtctttt aatgctactt
     1381 cgctttaaag tggaactcag tttattttta ctatttattc tcaatctggt ttgcggtttt
     1441 gtgttgtggc tgattttacg ttttacttat ttttccaaaa ccctaatcac aatgcggact
     1501 gtaatttttt cagttgcttc agctttggtt ttaagtgtct attttaagct cttaatgtca
     1561 cttttgaagc aggcaaaagg ggcaaatact tttatggatt attttcttaa tgctttggtc
     1621 ttatttattt tcattggggt tggcatttcg ctggctattt tgattataac ccgcaaagaa
     1681 attaaggaaa agagtaaaaa tccgaaggag gacgaagaag acgatgattt ctgatcggga
     1741 tttctttgtt atcaaaccgc agataaatga acagggcaaa tttatatccc tgaatccggt
     1801 tgatttgttt cacaattccc atcccttgta tctggaaatc ggttgtggca agggtgaatt
     1861 tattgcccgc tattccatcc tgcatccgga atacaattat atcggattgg aagcggcaga
     1921 aaaaagaatc aacaacacac tcaagaaatt aacgccggaa caaaacccca atgtgcgtct
     1981 tatgcgtttg tatgttgata attcaatctg ccagctattt cctgcagaat cggtttccgg
     2041 agtttttatt caatatcctg atccctggcc caaacgaaaa catcatcgcc gtcgtttaat
     2101 tcaacaggaa tttttaactt ctctggcaat gattttaaaa ccgctagctc aggttcaaat
     2161 taccactgat aatagcgatt atgctgcctg gattttagat gagtttttag ctcatcccag
     2221 ttttatatcc gtctatgaag aacttatgct taaagaatca ccttttgaag aacatattcc
     2281 cacctggtat gaatccgaac aaaggcgaca gggctttgaa ccacaatata tgttatttaa
     2341 acgaatttag gagtaaacac agatgagtta caattttagt agcaactatc aaatcaacaa
     2401 aatgcttaat agtgttgtag aaagcatcac taatttggct gaggagcaat tattacatat
     2461 tcaaagatta acgcgtattg gtgaatcact ttcttcagaa accgacatag ataaaatatt
     2521 tgatatgatt ctggaagaag gaatttcctt tacccgagcc gatgctgcta caatttatac
     2581 ggttaatgag gatgccacca aacttcagtt tgaaattgtg tataatgcta ccttaaattt
     2641 acgcaaggga ggaacccggg gacccgtaaa ctggaaaagc attgaccttt acgatgaaaa
     2701 aggcaatccg gtagataaca aaatcgttac caatgtttat catcacaaac aaagcttatg
     2761 tttccctgat gtatacaaag cggaaggtta cgatgtgagc ggaactatta agaccgatag
     2821 agaaaataac tatcgctgta agtcaatgct aactataccc ttaaaaaatc atgaggatac
     2881 tgttttaggt gtaattcaat tcatcaatgc tatggatgaa gaaggaaaca tcatttcttt
     2941 taccgatgag cataaagcaa tgctttcttc gcttgcttcg caagcagcta ttgctctttc
     3001 caataaaaaa ctgattgaaa atttggaaga actattaaat caatttatcc gttccattgc
     3061 tggtgccatt gaaagaaagt ccaaatactc ttccaatcat ataactcggg tagcaatgct
     3121 gacggaaatg atagccacta aaattaacga aaagggagaa aaatatttcc cgggtcgcaa
     3181 attcaacgaa aacgaactaa aagaactttc tatggctggc tggatgcacg atgttggtaa
     3241 aattgtcact cccgaggcaa ttatggataa aagcacaaaa ttggaaacaa tttgtgaccg
     3301 catagagctg gtaaaattgc gtttggaaaa actggaacta ttgctaacct acttgcagtt
     3361 aaagctaaag gaagatgaat ttgctgcttt tatccatcaa aatatagaca gcgaactgga
     3421 gcccacgggt tttcgtaatt atcttgctaa tataaccaac ttccttatca atgtaaatgt
     3481 tggcaaagaa tttgttgata aagccgattt agaaaaagtg gaaaaactcg gaaagataaa
     3541 ttttgaatat gaaggaaagc gttactttat ttttacggaa gacgaaaaaa agaatatgca
     3601 gataagtggc agaggaacat ttactcctga agaaatgaaa attatgcaag atcatgttgc
     3661 catcacctgg gaaatgcttt ctcagctatc tttccccaaa aagtatgaaa atgtagcttt
     3721 ctatgcagct actcatcatg aagcgctcaa tggaaagggc tatcctaatg gctatacagc
     3781 agataaatta cctttgcagt cccgtatttt agctgtagca gatatctttg aagcattaac
     3841 cgcttccgat agaccctata aaacagccaa gaccttaagc gagtcattaa atattatggg
     3901 ttatatggtt aaggatggtc atctggataa agacctggta aatttctttt tggatagcgg
     3961 actttacaag gaatatgccg atcgttttat gaataaggaa tatattgatg aggtagatat
     4021 tgattccata aaagccatat acactaatca atgaccgttc cctttttaaa taagccgctt
     4081 ctatttttag cacctttagc tggctacagc gattccgctt ttcgccaaat ttgtaaaaat
     4141 tggggagctg aggtcttata cagtgaaatg gttagtgctg atggtataac cagaaattcc
     4201 gccaagacct tacaatatgt tcagtttgat gagtcagagc accctttcgg catccaaatt
     4261 tttggtagta atccactcat aatggcaaaa ggggttgagg tgttattacc tttaaaaccc
     4321 gattttatag atattaatat gggttgtccg gtgaaaaaag ttgtccgcaa gggagctggt
     4381 tcagctttaa tgcagacccc ttctttagct ggaaaaatag ttaaagaagt taaaaatgct
     4441 ctcttaggaa ggataccttt aagtgttaaa tttcgtagcg gatggaattt ccaaaatcag
     4501 aattatttac aatttggatt gatgttgcag gaatgcggaa ccgattttct ctgtttgcat
     4561 cccagaactt caagccagat gttttcgggc tccagcaatt gggaacagat aaaaatcctg
     4621 aaacaaaatg tccaaattcc ggttgttgga aatggagata ttaaaactcc ggaagacgca
     4681 caaagaatga taacagagac caattgtgac ggaataatga ttggtagagg tgctttaggg
     4741 aaaccctggc tttttgcgca aagtcgccag ttgatttcta ccggaactta caatcctgta
     4801 acccaaaaag aaatcgtgaa gaccattttt gcccatctgg ataaagcatt gctgtataaa
     4861 tcggaagatg ttgttgtgag agaaatacgt gcacatcttt gcttttatac taaaggcatt
     4921 ttaggaggag ctgaattacg caatcaaata aatcatacag aaaacattag cgaactgaaa
     4981 gcaatcatcc tgaagggatt ttcgcatata ggatagtgag ggctgagagc taagggcaga
     5041 gagatgaagg atacttgcaa gtcaggagtc gttagcgctt tgataacttt ttggttgtga
     5101 gataactttt ttagcgctta tggctcgtga cggcatattg acaggattta acgaatttat
     5161 cggatttaat ggaagttata cgaatttaca attgttaatt tgtcatcttt ttctcggtgc
     5221 tttcagtatt ctcggtgttc tcggtgtaaa aaaatctgcg taatctgcaa gatactggaa
     5281 acttcttgac actttgctca ggtttagaaa tagaaaccac agggtattac catacctgaa
     5341 taagattaaa aaaacaggga gaaaaaatga aatattttag aaaacttgta ggagaaaagt
     5401 gttatctttc ccccgtttct atggatgatg tggaaaaata cacggaatgg ttgaatgata
     5461 tggaaatagg gcaatttgta acactctctc atatagttct ggatatagaa aaggaaactg
     5521 aactgttgcg taaattgatg cgggaggatt atatttttgc cattgtggaa aaagatacta
     5581 ataaggttat cggaaattgt ggaattcatc aggtctcaca agttcacaga aatgcttctc
     5641 tgggaatttt tattggggaa aagacctttt ggaatcaggg tattggaact gaggcaacga
     5701 gtttgcttct ggattttgct ttcaacattt tgaatttgca taatgtttat ctttccgtga
     5761 tgtcctataa caagcgtgct attcgttgtt atgaaaaaat cgggtttaaa aaagtgggag
     5821 tacaaagaga atttatgttt gtttcaggac aatatcacga tgtttttatt tacgatatgc
     5881 tggcaagcga attcaccagt ccctatgtga aaaagattta tacctatagc atcagtgatg
     5941 aagcgggacg cagtaaaata agcattgttt aaatgttacc caagataaaa ggacaggaaa
     6001 atgccttaga acttttagat aaggcaattg aaaatgatcg tatagctcag gcatatcttt
     6061 tttacggaag tgatggagtt ggtaaattta ccactgccct ctatttcgga atggcagtta
     6121 attgtttagc taccagtgaa ttaaaaccct gtggcgagtg tgatgcctgt cataaatttt
     6181 tgcaattgga gcatcctgat ttcgtttatc tctttcctac tcccaatttg aatctcagtg
     6241 cagagggaga gattaagaat acggacgctc aaaaatcgta tgaagcatat atacagaata
     6301 aaattaacac tccttggaag gacttttttt tcaaagaagc tacggaaatc cggaaagaaa
     6361 gcattacgct attgaacaaa cgcctggaac tatccatcca tgaagcaaaa tacagaattg
     6421 taattattga agatgcagat atgatgaata atgctactgc taatgctttt ctaaaaacct
     6481 tggaagaacc tccagaacga actattataa ttctcattac tgaacgcttg ccattaattt
     6541 taccgacaat actttcccgc actcaacctg tttattttaa gcccttaaca aggggtgtaa
     6601 ttgaggaaat cctaatcaac gaatttgaaa tcaattccgc tttagccagg tctgcttcca
     6661 gaatttgtgc cggtaacttg aaaactgcca ttagaattgc ttcggaaagt gaatcccttt
     6721 ccagtaattg ggcttttgaa atcttttccc tggcagcaga agaaaatgat ttgggttatt
     6781 tggatttggc agaaaggtat aaaaaacagc aaaccataga ccaggtaatt gatttactaa
     6841 aatatatccg gattattgcc agtgacttag gttccctttc tgctaatcct gatagcgaaa
     6901 ttaccaatat agataaaata gatttcttga aaccaatcgc agaagcaaat ttaggaatag
     6961 atgaccggct ttatgatttc ctgctttttc tggaagacct caatcgtaaa gttgagggtt
     7021 atgccaattt aaatttagta ctgaccaacc tctatttaaa ctgtaaacat ttgctgaaac
     7081 cataaatgga agctaaaggc actaatcgga ctttggcaaa aaacatcagt gtgatgtcca
     7141 ttggtgtatt tatcagccgc attttcggtt taattagaga ccaggtgatg gcttatttct
     7201 ttggcacaac ttattggaat gatgccttta atgtgggcta caatattccc aatttattaa
     7261 gacgcctttt tggagaagga gcactttcca cagcttttgt tcctctctat aatgatatta
     7321 agattaagca gggtaaggaa aaacagatag aatttgcttt gaacctgctg agcgttttaa
     7381 cttttattct ttgtatctta acaattttag gaatagctct tgctccgttg attgtaaaat
     7441 gtctttatcc aggtttagca tctgaaacga aagttctggc tataaagctt acgcggatta
     7501 tttttcccta cctgtttttc attggcttat cttctacttt tattgccatt ttgaactcac
     7561 ataactattt ttttatgacg ggactttctt ccgcattact caatatcggg atgattgcca
     7621 cggttcttat tccccatttt gtgttaaaag tgagtggcga ggatttaatt gtttgggctg
     7681 gtgggggagt tttagtaggt ggttttttac aaactgtaat caatctgcct tatctgaaaa
     7741 gaattggtta ccgttgggcg atttatctta agtttggttc ggaggcattg tctgttctct
     7801 ggaagcgttt tattcccagt atgataggaa ttggaattcg ggaaataaac ttaattgccg
     7861 attcgttgat ggcatctttt ttaccaactg gttcaattac agcattaaat tttggcaatc
     7921 gtttaatgca attaccctta ggtatttttg ccatttccac gggaacagct gttcttcctt
     7981 tttattcacg ctgtgtttcc actgaaaatt atcaagagct ttcagaaagt attcgttttt
     8041 cgggtttgaa catagcttac attatgctgc cggtaacgac tattatttta gctttgggtg
     8101 aggactttgt acgcattcta tttgaaaatg gagcttttca gggagacgcg gttttgatga
     8161 cttcccaggc gttaatattt tattccttgg gtttaatttt ttatggcttg aatcaaacaa
     8221 ttactcctgt attttatgct tataaagata ccaaaacacc tgtgaaaatt gctgcgggaa
     8281 tggtagcttt aaatataacc ctgaatttta ttctgattca gtttatggct catcggggtt
     8341 tagctttatc tacttctata actgcttgtg taaatttctt tatactgctt ttcctgatcc
     8401 gcaaaaagat gccggaaata tctttttcgg ggattgccat aaacattatc aaatcccttt
     8461 tgatttgttg tttcttatac cttttgctct atataagctg taatcttttt cctgtaagcg
     8521 gcagaatttc tttactaata agagatgcca tactttctat aattgctctt gggctgtttt
     8581 atctcactgg tatcctgtta aaaattgatt atcttaatca ggcaggcaaa aaattatgcc
     8641 agcgttttct gaagaaatag aaacaaaact tgctttgctt cctgaacaac caggtgttta
     8701 tctctggaaa gataaagacg gaactatcat ctacatcggc aaggcattaa acttgaagca
     8761 taggataaaa aattatctta atcccactgg caaagacctt aaaacagaac atctgataaa
     8821 gaatattgcc gatttggatt acatcattac caatagcgaa gccgatgcct ttattttgga
     8881 agcaacttta gttaagcgtt accgtcccaa atataatatt atgctgaaag acgataaacg
     8941 ctatcctttc gtaaaaataa cggttaatga gccctttccc agaatcttta ttacgcgtga
     9001 gttgctgaaa gataatgcca aatattttgg tccttacaca gaagttcgct atttaagaaa
     9061 tactttacgc gatttggaat ggatttttcc tttacgtagt tgcaatagaa atattcctaa
     9121 aataaaatat aaacaaccct gtatgaattt ccagctgggt aaatgtcctg ctccctgcat
     9181 cggaaacatc agtcagaagg actatgccaa gaccgtaaat cagctcttgc ggtttttcaa
     9241 tggacgctat acagaacttt tagatgaact gagaacagaa atgcaaaatg cctcttcaga
     9301 actccgtttt gaggaagcag gaaaattccg tgaccgcatt attgccattg aacgcattca
     9361 aaaaagacaa tccgttttca atgtggaagg cggaaacatt gatattatcg gcttttatag
     9421 agaagaaaaa agagccgtct gtgttattct gaaaatgaaa aacggagcta ttattcatca
     9481 ggaaaattat ccgcttacaa atttggatta tgaagaaccg gatagtatcc tggcgagctt
     9541 tttacaactt tactatgctg atagagaaga aatgccggaa gaagttctgt taccttttgc
     9601 acctaaagat atggataaac tgaattcctg gctgggcaat aaattgcaga taccccaaag
     9661 aggagaaaaa accaagctga ttgcaatggc aaaaaccaat gcctttaaca ttgtagaaga
     9721 aagcaaactg gcacatttaa gaaaagctaa tcggactatt ttccctgttc aggaactgaa
     9781 agaggcctta aaattgccta aactaccacg gaaaattgtt tgtatggata tttctaccat
     9841 ccagggaagt gatactgttt cttctgctgt cttttttgaa aatgggaaaa gcaaaaagaa
     9901 atactatcgc catttcatta ttcgcagcat tgaaacccaa aatgactatg ccgctatgca
     9961 ggaaacaatg gctcgttttt taacggaaac ccaaaaagaa ccccaaatga aaccggacct
    10021 catcgttatt gatggaggaa aaggacagct ttcagccagt gaacaaatat tacgagcttc
    10081 cggaaataac gatatcagta taattgcctt agccaaaaga gcagaggaag tttttgtccc
    10141 gggaaatgaa caatccatca ttttaccgcg ttcatcttcc gctttacgat tattgatcaa
    10201 tatccgagat gaagcacatc gtttcgctat ctctttacat cgcaaaagac gctcccgcag
    10261 aacattggaa agtgaattgg aaagtatttc cggtatcgga gaaaaaaata aatttctgct
    10321 gttgaaagaa ctgggcagcg tggaaaatat tgccaacgcc aataaggaaa ctctgtctaa
    10381 aataaaaggt ataggaccta aaatcgcaga gaagatatat aaccattttc acagcacata
    10441 aaattccttc ccttttttat tgcgcttctt aactgcatta ctgaagcatt gcttacccaa
    10501 tactaatccc atccgtaatg ggtaagcaat gggtaagcaa tgctattaag gaagaagtta
    10561 tggggaatac ataatttatt gcaaatcaag tggttgcaag gtgaagagtg agagttaaag
    10621 tagtgagatt ttaaacaaag agaaaaagag aaataatgga gaatggagaa tgaacggtga
    10681 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgtaatc tacaggaaac
    10801 aaatacaaaa aattttttct caatttatta tttattgccc ttcagatagt tacatccatt
    10861 accctaaaat agcaaatact acatataaca attctaactc caagtataac aaaataataa
    10921 acattcgtct ttcaactgaa ataaggagaa cagtaattta gaaatttgaa aaaaaatgca
    10981 ttttgcactt gacaagaaaa acgaagtgtc atataagaaa cacaggaact cagaaaaact
    11041 caaaaaatct caaggtggtt cacggaaatg agacaaaaaa gcacggacag taaagcaaag
    11101 aacggttttt ttaggaaacc ggaaaaaaag gatgtcagat atgtctggtg aatttcttgg
    11161 tatctttgaa aatgctgtgc ataaacaaag ggtaataatt ccagctgctt tcaagaagaa
    11221 attttcggaa gaagcggaaa aaaaggttgt tgttacttta ggtcccaata atacgattgc
    11281 catttatcct ttggattgct ggaaagcaac actggaacgc ttaaaaaacg gtgatgaaca
    11341 tagtcataaa cttcgcactc aattaattga ttttgcgatg acggaacagg aattggaagg
    11401 acctggaagg gtgcgcattc atgaaatgct attaaatgaa gttaatatta cggatagtgt
    11461 tgttattaaa ggtgaaggtc attatatttc actttggaat cccaaggttt ataatgaagt
    11521 gcgtgccagt aaacttgatc aacatcgtaa acaattcact tctgaggatt atcaattatg
    11581 acttttcaca ctccggtgat ggttaaagaa tgcctttcct acttaaattt gagagcagga
    11641 catatttatg tagatgccac aaccggaggt ggaggacata gtttagctat gctaaaagcc
    11701 caacctgaaa ttaagctatt ttgttttgac caggatagtg aggcaataga agaagcagga
    11761 aaaatacttc ataactataa taatgtagaa ctgatacaag ctaattttaa acaattacgc
    11821 acggaacttg cctatcgtaa aataaaagga attgatggta ttctttttga tttgggtgtt
    11881 tcttctcacc agctggaatg cgcggaacgc ggtttcagtt ttgaaaaaga tgctccatta
    11941 gatatgcgaa tggataaaaa cctgaagaac agtgcctata atgcggttaa cgaattggac
    12001 gctaaaacac ttaccaatat tttcagagaa tacggagaag aacaaaatgc ctatcgtatt
    12061 gccaaagcca tagaaaaaag ttccaaacct ataaagacca cagttgaact ggcaaaaatt
    12121 gtagaaagcg tagcaggaaa aggaacaaaa gcatcattaa aaaccaaagt tagagttttt
    12181 caggcattaa gaatttatgt aaataaagaa ttggaatccc tttcccttgc tttgcaggat
    12241 gctatcaatc tgttaaatcc ccaaggcagg atagttgtta tcagctatca ttcattggaa
    12301 gatagaattg tgaaacagac atttaaaaaa gcggaacaga aaaatgaaga gcagtcggtt
    12361 ttacctaata gtaataagaa ccatcagcag cagttaatta ttttaaccaa aaaacctgta
    12421 acagccacgg aagaagaaat tttagttaat ccccgttcac gcagtgcaaa attgcgtgcg
    12481 ggagaaaaag tgcaaaggga aaaaagagaa acaaaaacca gtcggaaacc aaaccgacaa
    12541 aattcgcagg aggaaaaatg agaggaagaa tcctgattct cgtgtcccta ttcgcaattg
    12601 tattttttct caatttttat aacaacaacc gcattgtgaa ttataccaga caaatatctg
    12661 ctctggaaaa gacctattat gcggaaaaga acattaatac ggaattactg gtagagcttg
    12721 atgatttacg cagtggaagg catattgctt ctttagtaag agtggaattg agcaatttca
    12781 ttcccgaaaa acaagcaggt aaaattattt atgtgcatga gccctctccc aaacaggaaa
    12841 agaagaccta ttgcatcata gacctgttgt ctaaaaaagc agaagccaag aatgtgcaaa
    12901 ttttgctgga ctaagatgaa atcccgttat tactttttag ttgttatttt tgctctggta
    12961 gctgtgatct ggattgcata tttattctgc attcagatat tagacccttt tcatctggca
    13021 ggcgcaagaa gaatgcgtta tacaccccaa aaagaaattc tgattccgcg tcggggctct
    13081 attttagatg cccaaggtaa tcttttagta agctccgtaa tctattatca gatagatatt
    13141 gaccgcagtt ctgttgcatc ttgggcaaaa gaagaaaaaa tacctttaga aaaagcattt
    13201 gaaaagtatg ccgaggttat atcctctcac accactctaa ataaagatga tgtaataaag
    13261 cgcttgaatt atggaaataa aaccagttct attcaaataa gcaataaaat aagcgaagtg
    13321 gaactggata aattaatcaa ggaactaaag acaaaggata tgcccggttt ggttcacagc
    13381 tttgcctcaa tgaaaagaat ttattctaag gacattttag ctgccagagt tttaggttct
    13441 gtgcgcgagg tctccgatgg ctatgatcct gctacaaata gtaaatccct ttacaaactt
    13501 gccggggtct gcggaattga atctacttac gataaatacc tttccggaaa ctacggttgg
    13561 aaagaagttg ttttagatgc taatcatcaa cctgttccct atcctaatct gcattccaaa
    13621 aagccggaaa acggatataa tttatattta actattgatt ccaagataca ggagattgtg
    13681 gaaaatgccc tttacgaagg gatggaacat tatgaagcca aaaatgccgg cgctattgtt
    13741 atggacccca atacaggtaa aattttagct atggcaggtg tttccggaga agataaaaca
    13801 gaagacccca attttgtgag ggtaaaatcc aatattcctg ttagttttat gtttgaaccc
    13861 ggctctacaa tgaagccctt aactatgcta ccagcaattg aacacaaact cgttaaacca
    13921 actgaaacat ttcagtgcgg tagatatcaa gtagggcgaa gggttatttc cgatacgcat
    13981 caatatggag aattaactcc ccgggaaatt ataaccaaat ccagcaatgt aggtattgct
    14041 aaaatagctg aaagaattgg acctacgcgg ctttatgaaa atttcatcgc tttgggtttt
    14101 ggtcaaaaaa cagctttaaa tttggcaggt gaatccagcg gaatgtttgc caaactgcaa
    14161 aattgggatg gctatacttt acattccata gcttttggac aaggaatttc tactactgct
    14221 ttacaacttg ccactgccta tagtgccata gccaatggtg gaaaactgat gaagcccata
    14281 attgtggaaa caataaaaga tgacgaaggc aaaattattg aacaatttga gccctccgta
    14341 ttacgcaatg ttgcctctac cgctgctacg gatacaatta aatcctatct acaaggagtt
    14401 gtagattccg gaactgccag acatataaaa atggattata ttcaggttgg cggtaaaaca
    14461 ggaaccgctc aaaaaaatat aaccggcact aaaggttaca gccagggaaa atacagttcc
    14521 gtatttatag gtatgtttcc tattgaaaaa ccccagatgg tagttgtggt cttttttgat
    14581 gaacccgccc ccggttttca ttatggcagc acaagtagcg ctccaacctt taaaaagatt
    14641 gtggaaaata tcctctttat gcccgattgt cagattttac cctataatga acgcttgatg
    14701 cagacctcgt taactatgcc gaaattaacc ggaatgcatc tctttcaagc agaaaataca
    14761 cttagccgtt acggttttca atataaagtg gaaggacccg attctgcttc cattgttata
    14821 gatcaatatc ctaaagctgg agttacggta gacccccatt atcctataac cttaaaaata
    14881 ggacccaata ccgataaaaa agctcccgtt tatgaaacag gcacgatgcc caatttagta
    14941 ggacttagtt tacgggaagc attaaaacag gcatcaattc aaggactggc agttaatatt
    15001 cgcggttcag gaatggtgcg ttcacaatcc gtgcttccag gaagtagaat tcttaaaggt
    15061 agtaaatgct atctggaggc atcgttgtga taaacagaat cctatatgcc cttcaacagc
    15121 attctctcct tgtggaaaca gcaaatttgg caaagctgga aaactggcaa ggaaaagtgc
    15181 agacagataa ccggaaaatt aagcccggag atatatttgt ctgcattcaa ggtgctaatt
    15241 ttgatggaca taacttcatc aattccgctt tggaaaaagg tgccgatatt attgtctgca
    15301 gggaaatcaa taaaagagat ataccctata ttaaagtgaa agatactcgt aaagcagccg
    15361 ctgttattgc cagaaccgta atgctgccgg aaaaaattcc ttatacatta ataggtataa
    15421 ccggaacgaa tggcaaaact acaacttcct cgttactcta tgaagcatta agaaatctgg
    15481 gcttccgttg cggctggatt ggaactttgg gttactatat tgaaggtaaa gcattcccct
    15541 ccgaaagaac aactcccgat atcattgaac tgaatgaaat ttttgggcaa atgatagaag
    15601 cgaaagttga ttatgtggtg atggaggtct cttctcattc cctttctctg gacaggattt
    15661 acggagttaa atttgactac tgtctattca gcaatctggg acgcgatcat cttgattttc
    15721 atcaaaccct ggaaaattac ggcaaagcca agatgagctt ttttgaacaa ggagcaaaaa
    15781 atggcagtat atctgttatc aatacggctg atgcttttgg agtgcagata aaggatatgc
    15841 tgaaagaaaa gcaggcaaaa gtttattctc ttgggggcaa tgatgccgaa tttcaatact
    15901 cggatataaa aacggatata aacaccagca gttttaagct gattgcggaa aattgtgaaa
    15961 ttccggttac cagtcgttta attggcagtt tcaatattca aaatctgggt ttaacagctc
    16021 taacattata tgctttagga ttcagtcctg aggcaataca caattctctg aaggatattc
    16081 ctcccgttca aggcagattg gaacgagtgg aaaataactt taatatcggg atttttattg
    16141 attatgccca tacccccgaa gcaatagata atgtgttaaa agcagtggaa gatttgcctc
    16201 ataaaaggat tctctgttta atcggcgctg gaggcgatag agataagggt aaaagacctt
    16261 taatgctgaa atcagcttta aatcatagtg atgttgttat tatcagtgac gataatcctc
    16321 gtagtgaaaa tccggaaagg attattaaag atattgtttc ctcttcagat atctttttgc
    16381 cttggtggat tatcagaaat cggcatcagg caatttcttc cataatccgt cttgcccaag
    16441 aagaggatat agttcttatc tgtggaaaag gaagtgaaga ttatcaggaa attgaaggtg
    16501 tccgccatca ttttaatgac cgcgaagagg ccttgaaagc tcttgccgaa aaagaaataa
    16561 aagataaagc cgatgacgaa ttgattttgc cgatagatca aaccctgttg aaattattac
    16621 tcttgccaaa ttgggaaccg gaacctaagg gataccaaga aacatattct tattattatc
    16681 tttctataga ttcccgttct ataaagccgg gtagtgtttt tatagctatt aaaggtgaaa
    16741 aatacgatgg acacagctat ctggaagatg ttcttaaaat taaagagaat tgtgcaatcg
    16801 gggaaatacc ttatccgctt aatcaattct cagaggaaaa tagctattat caagtggaaa
    16861 attgccttga gacaatgggg cttatctgca gaaaatattt acagctgttc ccggcaaaga
    16921 gaattgccct cacaggaagc acaggtaaaa ctacttgtaa agaattaatg gctcaagttc
    16981 tgcaaagctc agctcccgtt ttaaagacac tggcaaatga aaataatata atcggcttat
    17041 gtaaaactat tatcagaatc aagccggaac agcagtatgc aatctttgaa ctgggaacca
    17101 atcactttgg agaaattgct gctctggctg atgttgctaa tcctgatatt ggtattattt
    17161 tgaatatcgg accttctcat cttgaatatt tcggggatga agaaggcgtt tttagagaaa
    17221 aaatcactct ttttaagaga ccgcttgctt tgcgtttata tcctggcgat gattttcgct
    17281 ttgattatct tgccaatgaa ggaataagta tcggctacaa tgagaattgc gattatagaa
    17341 taactcggca taaatgtaat aaagataatc aatcgtttta cttgaatgag gacttgtggt
    17401 ttttacctta tggagcgcca cactatgcaa taaattctgc ttttgccatt gttttgggat
    17461 taaaactggg aatagacaaa gaaaagatgc aaacagcatt aaataaacct attcagctgg
    17521 aattgagaag tcatgtagaa aaaagaggaa caggattatt aattctggat tgctacaatg
    17581 ctaatcctta ttcaatgcaa aaagcactgg aatattggca ggaactaaat ccggaacaac
    17641 ctcattatgc tattttgggt gatatgctgg aactgggaga aagctctatt ttatatcacc
    17701 aaatgatttc cgcaatgtta gcagaaatgc gttacgaaaa attatttact gttggaaact
    17761 attcccgtat ttatcatccg gaaaaggaaa gccctatttt gcattacaac acagtggaag
    17821 aactgattgc tgaccgaaaa ttgaattctc ttcccgaaaa tgccattatc ttagtaaagg
    17881 gctcacatgc aattcagttg gagcgtgtaa tccctatctt gaaaggagag gactaatgtt
    17941 ttatcatctt ctataccctc tggcaaagta tagcattgtt tttaatgttt tccgctatgt
    18001 aaccttccgt tccataggag ctttcattac ttctttaata atatctctat tggtaggtcc
    18061 tgcctttatt agaatgctga aaaaaaaatc tgctgtagag acaatagatg aagaccttcc
    18121 ggaaaggcat cgtttgaaac agggcactcc gacaatggga ggtataatta ttctggtttc
    18181 attgctgatt tcttctcttt tatggaatgt attaacaaac ccagcaattt taatgatcat
    18241 tctaactacc gtttggttag gtatcttggg ttttttagat gattatttga aaaattatgt
    18301 gaaagctaaa aaaggactaa ttccccgtta taaactttta ggtcagattt ccgttggttt
    18361 aattcttacc ttggcaatct actacagcag ctccgtttcg gaaaatatta ctgctatgca
    18421 aattcctttt ttcaaaaacc tgatagttcc cttgggctgg gttttcattc cttttgcggt
    18481 tctttttata accggaactt ccaatgctgt aaatttgaca gatggcttgg atggattggc
    18541 agcaggaact ttaatctttt cttttttggg cttgggagtg atgagttatt tgaaaggaaa
    18601 ctacatagca gcgaattatt tgaatctgga atttatttcc agtgcgggag aactgactgt
    18661 tttcatcagt gctataatgg gaactttaat gggttttctt tggtttaata gctatccggc
    18721 agaagttttt atgggagata ccggttcttt aaccttagga ggtattgccg ctgtaatttc
    18781 tattttgctg aaagaggaaa ttttcttttt tattcttggt tttatctttg tatatgaggc
    18841 acttagtgtt attattcaac gttcctggtt taaatataca aagcgcaaat acggtgaggg
    18901 gcacagagtt tttctttgtg ctccggttca tcatcattac gagctgaaag gtattcacga
    18961 atccaaaata gtgatgcgtt tttatattgt ggctgccttg cttactgcag ttgccttatc
    19021 aactatcaag ctacgctaaa ggatgctata gaatgataga taaagataaa aaatacggaa
    19081 ttataggttt ggctcgcagc ggaatcgctt ctgcctataa gctgaaagaa cttggtgtta
    19141 ttcctttctt aagtgaattg cgcccggcaa aagaaattcc tacagcagaa aaactggaaa
    19201 aggattttcc ctgtgaattt gggggtcata ccaataaact gctggaatgt gattgtctga
    19261 tagttagccc cggtattcct ttaaatacac ctattctgca aaaggcaaaa gaaaaaaata
    19321 tagaactcat tagtgaaatt gagctcggtt acagaataaa ggcaaaggac tctaaaatta
    19381 ttgctgttac tggttctaac ggaaaaagca caaccgttag cttaattcat catattctta
    19441 caggattagg caaaaaatcc cttttagcag gaaatatcgg cgacgccttt tgcagttatg
    19501 atatagaaaa acccgggtgg gactatatcg ttttggaaat cagcagcttc cagttagacc
    19561 tgattaacag tttccaaccc gatgtagccg tgcttttgaa tattacaccg gatcatatag
    19621 accgctataa ttcctttgag gactatgctt taagtaaggt aaacattttc aaaaatcaag
    19681 aaatgacaga tactgcagtc ctgttttggg attcccctga agttaaacat ttcactaagg
    19741 atataaaatc caggaaacta tatttctctt tgtctccaaa cgataacgca tttgcttatc
    19801 tgacaaataa ctatattcag tttggcagcg aaaccaaagt tccgctttct tattttgcca
    19861 ttaagggacc gcataattat gccaatgcga tgtctgcaat gctggcagtt tatgctttaa
    19921 ttcctgaacc gacaaaaatt gccgaagcgg taaggggttt taaatcgctt ccgcaccgtc
    19981 tggaatatgt agataccata aaaggtgttt ctttttataa cgattccaaa gctaccaata
    20041 ccgattctgt gcgcagtgca ttaaacagtt ttgagcaacc gattcgtatt attatgggtg
    20101 gttctgataa aggagaggat tatagtgtgc ttgctgctga tttgcaaaaa aaagtgaaaa
    20161 aagcatatct gacaggtgat acccaggaaa aaatgcgtaa aaccttctcc ggtaaaatcc
    20221 ctttggaatg cattgctgat tttgagttga ctgtgcggaa ggcattttct gattctggtg
    20281 aaggagatat aattgttctt tcacctgctt gtgccagttt tgatcgcttt aataattatg
    20341 aggaacgggg aaatttcttc aaggaaattg tagcccgaat aggcaaagaa aatgagaaga
    20401 aataagacac caagttatat cgtcagtttt gataaggtaa ttttgatcac ctattgtctg
    20461 ctctgtctgg taggtttaat aaccttgctg gatatttctt ctgtccagag ttctatgaag
    20521 tatttttaca ggcaactcgt atttttgatt atatcaatga tcacagtgat tgtaattctt
    20581 tatactttta atctggaaaa actaagagtt ctttctcctt attttgttta tctcactatt
    20641 atcctgttaa ttattgttct gctaaaagga agcacagtta aaggtgctac gcgtcaacta
    20701 agtttgggct ttattaattt tcagcccagt gtattggctc ggttagcatt ggtcttttat
    20761 tttgctcaca tattggataa aaagtatgat gaacttgttg cttccaatcc acttcacttt
    20821 tttactaatt tttttgcttt aatagtaata acgggcataa ccttcctgtt gattattatg
    20881 gaacgccatt taagtactct aattataggt gggttaaccc tatatgctat gcttatttat
    20941 gctggcacta aaaaacgcgt gcttatttct ttagctctta taggcatcat agctggcgta
    21001 ttgattttag ctaacggagc agattataga aagggacgtt taacaactta caagaaattt
    21061 agtttattct taagacccga aggtgaaatc aaaattgagg atagtgatta tcaagtgaaa
    21121 gaaagtttaa ccgctctttc cagtggaggt cttatcggaa ccggtatggc aaaaggaaga
    21181 gcaaaacatt attatcttcc cgaagccaga acagattatg tttatactgt aattggagaa
    21241 gagtggggtt ttattggagc cctaattgtt tttggtttac attgttttct ttttttccgt
    21301 tgtttccgga tggcaaattc acaagaaaac cgatttttaa ggtttttagg tgtgggcttg
    21361 gcaatgaata tcttttgcaa tgtgttagtc aacaccggag tagcaatgtc cattttaccg
    21421 ccaacaggaa atactttacc ttttataagt tatggtggat cggctttact tattgattct
    21481 atcgctttag gaatgcttct aaatatcagt gctcagcgga gatatgtatg aaattcattt
    21541 ttggtgcagg cggaacagga ggtcacatta ctcctgcatt ggcactggct gatgaattgg
    21601 taaggtataa gcataatgtc ctttttttag gtaatcgcca aagcattgaa gaaacgcttt
    21661 gtgcttcggc aggttatccc tttcggcaga ttaaagtgca gaaattacat cgttccttaa
    21721 aaccggagaa tatgctggtt cccttttatt tgttaggcag tgttattacc gctgtaaaca
    21781 ttatgaaaaa agaaaaaccg caggcagtta tctgcaccgg tggttttgta gctggtcctg
    21841 tagccatatc tgctgcctta ttaaaaatcc ctttgttctt ccatgaaagt aatagttacc
    21901 ccggcttggt aacccgtaaa atggcaaaat ggattaacac tattttcatt tcctttgaaa
    21961 acagcaggaa atatttaagc cgtgcaaaat tggtaaacta tggaataccg cttagacaga
    22021 taacaaacaa ggatcagaaa ccttttgaac ttagctcttt gggcttgaaa aacgataaac
    22081 caacgcttat agttagcgga ggaagtcaag gttctgttgc tataaacaca gttgtcaatc
    22141 aggcaatttc ggatatatta tctttgggct atcaaattat ttggcaaacg ggaaaaataa
    22201 gttacgagcg tttttctgcc aaacataagg gaagggaagg tgtttatatt tttgcttttt
    22261 cgcctgacct gcccaacatg ctttcccaat gcagtttagc tattacgcga gcaggagcta
    22321 tgactattgc ggaactggaa gaaaatcgtc ttccggctat tcttattcct ctgcctacag
    22381 ctgctgaaaa tcatcagtat tataatgctc tggctcaaca ggaaaaagga gttgcagtgc
    22441 ttttagaaca aagtaaatta actccctttt cccttgtaga aaacatcaaa aaaataactg
    22501 aaaatctgga atattaccgg aataagttag cttcgcttcc acccaatcag gcagcccaaa
    22561 aaataattga gtacatttta aactatctta atagtaaaga ggaggaagaa tgttaggaag
    22621 aaccagaaaa atacactttg tcggaattgg aggtatcgga atgagcggaa ttgctgaatt
    22681 tctgcacaat cagggtttag aaattaccgg ctccgatctt aaaaaatcgg atattacaac
    22741 tcatttggaa tccctcggca taaagatttt tgaaggtcat aaccccgaaa acatcggtga
    22801 tgcagatgta gtagtaaaat ccagcgctat aaatgatgac aatccggaaa ttatatctgc
    22861 caacgaaatg aaaatacctg ttatccgcag agcagaattg ctggctgaaa ttacccgaat
    22921 gagtttttct atcggaattg ccggaactca tggcaaaaca acttccactt ctatggctgg
    22981 ttctgttttg gaaagtgcag gtttggaacc aactatcatc gttgggggaa aagtgaaaaa
    23041 ttttggcagt aacaatgtga tgggcagtgg aaaatatatc gtagtagaag cggatgaata
    23101 tgaccattct tttttaaccc taactccttg tattgcaggt attactaatg tagatgcaga
    23161 ccatctggat tgttaccgta atcttgatga aattaagagt tcttttatag aatatgccaa
    23221 taaagttcct ttctttggca gtgttattgc ctgtttggat gatcctggcg tgcaggcaat
    23281 cttacctttt attcataaaa aaatagttac ctacggtttc tctcgtcagg cagatgtgca
    23341 agcaagagat attgatatgc agggtttcaa ttcttcctac gaccttttgt ttaaggatta
    23401 taagctggga cgcatttctt tgaaagttac cggtaaacat aatattcaaa attcattact
    23461 ggctgcatcc atcggtctgg aattggatat cccttttccc gctattcaag atggcttagg
    23521 taaattcagc ggtgtttaca gaagatttga tttgaagggt gaagcaggtg gaattaccgt
    23581 ttatgatgat tatgcacatc atcctacaga aattatagca actttggagg gctttaaaga
    23641 tagcgccaaa aggagaattg tagctctatt tcaaccccat ttatactcta ggacccgtga
    23701 tttttatgag gagttcggaa agtccttctt ttcttgtgac tgcctcattt tagctccaat
    23761 ttatcctgca cgggaacaac ctattccggg aattagttcc aaactgattg ctgatgctgc
    23821 aattcaaagt ggacatcata atgtccattt aattaactcc aattctgaaa tagtgagcaa
    23881 aacccttgct cttcttaaag aaggagatat tttaatcact atgggagcag gcaatatttg
    23941 gcagtatgga gaagaaattt taaggcaatt gaagaattat gttgacacaa atcataagca
    24001 ataaaatatt agtaacttgt aaagctaaaa tcagtttttc aaattttact gttagtaagc
    24061 taaaggactt tgatacataa acttatggaa ttaaacaaag gtactcgtaa gcgtcgcggt
    24121 aatagtcgtt attacctgtt cttctttttt gccttaattg gcgtagcgct tttgggaacg
    24181 ggtatctggt ttggtcttac gcatatagac ctgtttacct tacaaaaagt aactgttatt
    24241 ggaaacgaag caattcctga cactttaatt tacaaaatta cccaacccta tataggaatg
    24301 aatttactgg caattcctgc agaggatatt aaaaataagg taatgaatct ttcccgggta
    24361 aaggatgtta aattacataa gcgtttacct tcaaccataa aactggaaat caatgaacgc
    24421 aaagcggcaa tatatctaaa aacaattgaa ggtgatttgc atccaattga ttccgaagct
    24481 gttgttttga tgaagtattc ccctatttat aaagaagata tgccaattta tagcacctat
    24541 ttaagtaacc atcaaataaa acctggacat aaactgaaaa atgcaggatt gcaacaggta
    24601 ttacaattgc ataaacgcat taccaaagaa gctccggatt ttcttcccca gatttcggaa
    24661 tattatttaa tagataagac ggttaatatt attgatgcga aaacaggtac acgcattatt
    24721 cctgctcagg aggacttggc taaacagctt gcgcgttatc agtttgttca ggaaaatggc
    24781 aatataaaca aaaatagcgt tgtggatttg cgctataaaa atcaggttgt agttaaggca
    24841 ggtaataaat gaaacaatca atcataactg ctttagatat tggctcttcc aatgttcgtt
    24901 gcatcattgc caaagaagtt ggagatggac gtctggaaat tttaggttta ggcgaatatc
    24961 cttccgaagg cattgaaggt ggaattgtta aagatattca ggcactttcc ggttgtgtag
    25021 caaaagcaat ttctacagcg gaaaaagaag gaaaaacctc tgccaataat atctattgca
    25081 atattaccgg agaacatatt cggactcaat ttggagacgg acgtatttcc atcccttccg
    25141 aaacaccgaa tgaaccagga gaaattactc aagaacatat tgaacaggtt atcaatgatg
    25201 ccaaaaacag cgttaagatt cagaaaggtc tggagcgttt tcgcattttg cacggaattc
    25261 cgcataactt tgtaattgat aatcaggatg atattcacaa tccagtgaat atgaatggat
    25321 ttcatttaat tgccaaggtc tatactattt tagccgaact tacccctttg cgcaatttaa
    25381 gtaagtgcat tcagctggca ggttatgaaa tcaatcctga aaattttatt ttaaatcata
    25441 ttgctatttc cgaatctgtt ttaagtgaag atgaacgacg gctgggagct ttagttcttg
    25501 atattggagg tggaacttgt gacttatcta tttataatcg cggttccctg gaaaaaatcc
    25561 tcgttttgcc gatggcagga aaaaatatca cagaagacct ggcaattgga cttaagacca
    25621 ctttaggcaa tgcagaatat atcaaagtgg aatatggaaa cgctcttgcc agcagcgttg
    25681 accaaacaga agaaattgat gtggaaggaa ttagtggacg ctctaccagc cgcaaaacta
    25741 aatttttagt tagccatgtt attcaacacc gggtagaaga aatgctttct ctttgttaca
    25801 ataaagcaaa ggaattttac actccggaac tggtaacttc gggtatcatt ctttgcgggg
    25861 gaacagccaa actgaaaaac atagaggttg ttttatctga agcattcaat ctccatgtaa
    25921 aaattgccac accagacctt tcccgtttaa acggaatgat tagccgtttg gaagacccgg
    25981 cttatgcaac cgttgtagga attttatact ttgcggcagg taaaaataac gaacccgtta
    26041 gtaagggctt tactctgcca aacataaaag gaaaaaaaat cgtagataaa gttaaaaaaa
    26101 tattaaagga ctttacctaa aagggggata aaatgattga atttgacgaa aaacccgccc
    26161 aaatcggaac caacattaaa attatcggtg taggtggagc aggtggaaac gcactcaata
    26221 ctatgattga gaataattta ttcggtgtgg aatttatcgc agctaataca gatatccgcg
    26281 atcttaccaa aagtaaagcc aatatgaaat tgcaactggg aaagaaactt acccgcggat
    26341 taggaaccgg tgccaatcct gaacttggag ccagaagtgc agaagaatcc aaagaggata
    26401 ttaaaaatca tctggacggg gcagatatgg tttttattgc tgcaggaatg gggggtggaa
    26461 caggaaccgg tgcctcacct attatcgcta aaatcgctcg tgaaatggga attttaactt
    26521 ttggaattgt aacttctcct ttcccctatg agggtaaaaa aagagcagaa aatgcaattt
    26581 acggcattaa acatctacga gaattcgtgg atactttact agttattccc aacgaaaaac
    26641 tctgtgaaat ctatgcaaac cttactttaa aggaagcatt taaaaaagcc gaatttgttc
    26701 tttatgaagc agcccgagcg gttagcgaca ttatcaatgt tacaggtctt atcaatgtag
    26761 attttgctga tgtgaaagct attatgcaaa atatgggtta tgctcttatc ggcagcggaa
    26821 ttgcagaagg agaaaacaga gctataaacg cagccagagc cgcaattgat aatcctcttt
    26881 tatcgcatat cagtttacag ggatgtcagt ccttactgtt aaatatcaca gccggttatg
    26941 atatcctgat gagtgaattt gatgaggtct ccaatgttat tgtaagtgaa accggaaaag
    27001 ccgctaatat cattatgggt attattctgg atgatgctat ggcaggaaaa attcaggtta
    27061 ccattattgc caccgggctg gaaaaagaag acaaagaaca tactattgac ttccccggtt
    27121 tgcctgaatt cggagaaaaa tctgtccctt ctatcactca atctgaagat accaattccg
    27181 aaattgaaga tatctttgcc cgtttgagta ttaaccaatc aacaccaaaa gaggaagtta
    27241 aaccagctgc gaatgaacct acacgcattc ctcctttgaa aacagatgtg ccttcattcc
    27301 tgaaagcact tgattaaaat taaaaaacgc ttcatcataa actcctcctt gtttatgtga
    27361 aacaaaagtc caaagctcac taaccgtcag cacccccagc tgacggtttt ttttctttca
    27421 caaagagaga aagagaaaaa gagaatattt ttacaaagag aaaaagagat aaagagaa
//
LOCUS       DAOOZV010000022        27448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_1908_length_27448_cov_63.104443, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000022 DAOOZV010000000
VERSION     DAOOZV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27448
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_27448_cov_63.104443"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1707)
                     /gene="lysS"
                     /locus_tag="PLW08_03665"
     CDS             complement(211..1707)
                     /gene="lysS"
                     /locus_tag="PLW08_03665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPL51732.1"
                     /translation="MSSKPNQFIQLRKEKLAKIKALGIDPYPIKSYRTHYISDLLKDK
                     EEQLQKAEEVTITGRLTAMRRQGKIGFGDLEDISGKIQLYVAQNLIGEENYELFKLCD
                     PGDFIQATGKTFFTQTGEYSLKCNNIKLLSKNIRPLPAVKEKIVDGKKIRYDEFSDIE
                     LRYRKRYLDLLLNPEHRKVFETRAKIITAIRNFMDSRGYVEVETPILQPLYGGANARP
                     FITHHNTLDMDLYLRIATELYLKRLIVGGFEKVYELGKDFRNEGMDRTHNPEFTMIEF
                     YEAYSDLEGMMDIAEELIKHLALDIIGKDTFFYHNHQVCLSGEWKRASLPDLVSEFVG
                     QNVMNISFEELSAICKNKELVIPPGSEKGKLIALLFENFVEETLIQPTFVRDFPKEVS
                     PLAKAKPENPFVADRFELFIAGGEFANAFSELNDPLEQRVRLEEQAKLRALGIDDANV
                     VDEDFLEALEYGMPPMGGQGIGIDRLVMLLTENDSIKEVILFPQMKTT"
     gene            complement(1717..3228)
                     /locus_tag="PLW08_03670"
     CDS             complement(1717..3228)
                     /locus_tag="PLW08_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPL51733.1"
                     /translation="MSDKLHTTSSKAAMKEMKEEYLRMLEESFQNTAEIKKGDVIEAP
                     IVSIGDQYIILNLGGKFDAYAEIGEFSDSRGVLPYKVGDNLKGYVVDQNENGYVVARS
                     LTKQYVDKQSILEAFEKKIPVQGKIYTPTKGGFNVDILGARAFCPVSQVSLHPVEDSS
                     QFIGKTLDFLVIECSENCRRIVVSHRQLEEMREQERKEMALAKLKVGDIVKGKVLRMT
                     TFGAFIDLGGIEGLMHVSEISWQHIVRPQDELKKGQEIEVKIMDIKGEKIALSRKVLL
                     EDPFEVAMKELHEGDIINCRVLRLHNFGAFAELKPGVEGLIPISEMSRNRNISHPRDI
                     VKEGDWVQVQILRIDEDTHKISLSLKALEADPWDKIDEIIQLETPFEGIVESSTNFGV
                     FVTISEGITGLLPKSRVRKNDTFQTGEPVTLMVTAIDKENHRITLDYTDRSPVELAEE
                     RVRSEEHNYRDQHTEKIRSSNPRRGGRNRSDEEWRKYANQKVNPAADNPFKDL"
     gene            3485..3961
                     /gene="nuoE"
                     /locus_tag="PLW08_03675"
     CDS             3485..3961
                     /gene="nuoE"
                     /locus_tag="PLW08_03675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPL51734.1"
                     /translation="MKNTYPELDKILAKYQDKKGALIPLLQEVQKEKGYLSRETMQYL
                     ADKMKIPPAEIFGVATFYSMFRLKPQGKHLIRVCKGTACHVSDADGIKKAVMDALQLS
                     EEENTTADMQFTVMEVACLGCCSLSPVIMIDGRTYGKLTPGAIPAVLKQYEIEDTL"
     gene            3958..5727
                     /locus_tag="PLW08_03680"
     CDS             3958..5727
                     /locus_tag="PLW08_03680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPL51735.1"
                     /translation="MNNIIVKVGLASCGVAAGAKEVYTALEQYLENNPRPVILKKTAC
                     IGMCFEEPIVQFEGSELGSIHIGKANPETITHILEDYIAGKTPAENIILAEKISGKHN
                     ALLANQQRIVLRNCGIIDPECIEDYEAGDGYKALQKALQMERTAIIEEIKKSGLRGRG
                     GAGFPTGLKWQFCASENSDKKYIICNADEGDPGAFMDRSTLEGDPHSVIEGMIIGAYA
                     IGANEGYIYCRAEYPMALEHLKTAISAAEEKGYLGKNILGSSFSFDLHIKEGAGAFVC
                     GEETALMQSIEGKRGMPTLRPPYPAVKGLWGKPTNINNVETWANIPWIIMHGAQEFRK
                     YGTEKSPGTKVFALAGKIAGSGLIEVPMGIPLKDIIYKVGGGMKTSKPFKAVQMGGPS
                     GGCIPAEKLDTIVDYDSITATGAIMGSGGMVVMDSGTCMVDVARFFLNFTQNESCGKC
                     TFCRIGTKRMLEILTRITEGKGELQDIEKLEELAVNIIKGSLCGLGQTAPNPVLTTLR
                     YFKDEYLAHIVDKHCPAGVCNALITYKINPEKCIGCTLCARKCPVSCISGQVKQVHTI
                     DQSKCTKCGTCFQVCKFDAVTKE"
     gene            5739..9077
                     /locus_tag="PLW08_03685"
     CDS             5739..9077
                     /locus_tag="PLW08_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL51736.1"
                     /translation="MINVSLNGKPVKVEPGITILELCKQNKIDIPTLCNDEELNPYGS
                     CWVCLVEVKGRKGFVTSCGTTVSEGMEIITDSESIHKARKMALELLISNHYADCVAPC
                     TVACPDFVDIQTYVSLIANGKYHEAVKVIKEKLPMPLTLGRICPAFCEKECRRQIVDE
                     SIAIRQLKRYAADMDNEDVWNYVPERLPSKNKRVAIIGAGPSGLTCGYYLSNWGYEVI
                     IYEAAPKAGGWLRYGIPEFRLPKDVLDAEIELMCTNGMQIEYNKQLGKDIFLQDLAQD
                     FAAVYLAIGAQKAVPMPVKGSDLEGCFLGVDFLKAHALGNSPDLGLKVAIVGGGNTAV
                     DCARTAVRKGCEVSVIYRRTKAEMPAEPSEIAACEKEGVKFFYLANPVEYIGKGGHLT
                     SVKIEKMRLGEPDKSGRKRPEPTGEYFELPFDSIIAAISQVPEVDIFTQENNYVADKI
                     LPVSRWQTAIVDESTMFTGLGNIFAGGDFRRGAATAIEAIADGRIAATAINRFLETGE
                     ITAELMPFDSKKANTLQEVSPEEYSIYEKIERQEMPELPVIEAKNSFKEVELGFSEET
                     AKAEAKRCLECGCFVNETCFLRKYCTDYVVNPAQFLGDKNKHPIDYSHPFIMRDANKC
                     INCGRCIRTCAEIQGPAVLGYIYRGFATLVAPEFGDSLTKTSCLSCGKCIDVCPVGAL
                     MQRTVHYKFNPHPKDISLQNCGLCGVGCFIQTETQAGIVTRVTTPQEEPAFNGKNLCF
                     KGRFGWQSFYDKDRLKVPLFKENGIFKEISWQEATDLIAKEISLCSSKRFEISPYISL
                     EEMLMLQRAAHKYETELSANPVFSAFSDVFLNLSPQKEPYKILDDFEEYVVYGELNQV
                     LATLIRLQQRKGKKLTLVNYPENAFCRFADAVYANLAEVQATDKTLFIYNQNRISEQE
                     AFALWCFASEQGTDNLLVSTDFRNHLGFLAMQPDFSPSSSDFVLSWGVYPALANQAKF
                     SVAIMPFEDEKAPVDLLIPAPSFLEMEGTALSDLGQITKSANPANSIIINELMRLFYT
                     LNWIHPNSAEVPFWNQEAEKLLLTLQNYVPAKFEPSAVKIAELSKTIKFIPAQAEKYI
                     TALFEQGKKATSFSGRLISSSTNM"
     gene            complement(9220..10617)
                     /locus_tag="PLW08_03690"
     CDS             complement(9220..10617)
                     /locus_tag="PLW08_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HPL51737.1"
                     /translation="MKKILLTAINSSWSQSNPAIYYLREMIADLPYAVILQEWTLKDR
                     LWNVLSEIYCHKAEVICFSAYIWNRLYLQELIPEVIKLLPEAVIVIGGPEAESLSFLK
                     RKGLYIVQSAGEGKFRFLAEHDFSANDEDLSRAKHIPLKDIPFPYRESDGEILKGKLL
                     YYETFRGCPFACIYCLSANDKRSELRYQPNNQKDMQCLNKELETLISFQPRTIKFVDR
                     SFNLHKQLAHYIWKFFMKQNCPCDVHFEIYPDLLEEDDIALLEKAPENRIRLEIGIQT
                     INSEVALNCGRKSNWEKTKWTLQELKKRTNIRIHTDLLAGLPGENYASVLNGLNEVCA
                     TLPDAVQLGILKVLPDTPMQKIASELNYKWLSQPPYQCLSSDALTFEEIQQLENFAKL
                     LNLYWNKEEHKSMWQEMLQTQSATDILTALQQKHQELGYELHSLSKAKRNAVITDIYV
                     AGRHRPSAKSGKMRT"
     gene            complement(10910..11806)
                     /locus_tag="PLW08_03695"
     CDS             complement(10910..11806)
                     /locus_tag="PLW08_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPL51738.1"
                     /translation="MEKYIADFCNYLALEGKSPRTITAYKLDLEQFHSFIQRYFENSE
                     VDIKGITVLNIRDFFRFLNEKPDCNRSLARKSAALNSFFRYCKRSGFIQNNPMEKIKR
                     PKYEVPLPKFFTEEEVRTLLSIPDTDSPFGIRNKAILETLYSSGLRISELAGIRLQDI
                     DLKRGLVRVTGKGNKQRIVPLGSYAIEAINNYLKVRPQFVRENNPDLLFLTKSGKAFD
                     TKQLDIILKRYFELVAKAKGYSPHSLRHSFATHLLSRGADLRAIQELLGHSLLSTTET
                     YTHISLEDIKEAYKKGHPRSKE"
     gene            11878..12633
                     /locus_tag="PLW08_03700"
     CDS             11878..12633
                     /locus_tag="PLW08_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPL51739.1"
                     /translation="MNRFIDESAKIGMNVTLGNNVVIMAGVQIGNDCLIGHNVIIHPD
                     TKIGNACRIDDGTIIGKKPLSSPRSIFTVPTDLKGTEIGDFCQIGSNVIIYCQCTIGN
                     RNLIADLATIRENVTLGDLNIVGRNVTIENFVHIGNRNKLETNCYVTAYSEIGDYCFI
                     APCVATSNDNYMGRDKERFKHFKGVTMMTGSRIGVNATILPGKTIHSDGTVAGGAVVT
                     KDVPAKIIVAGNPAKPFSEVPEPQLLENNLDKQ"
     gene            12630..13346
                     /locus_tag="PLW08_03705"
     CDS             12630..13346
                     /locus_tag="PLW08_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPL51740.1"
                     /translation="MKTLIIIPTYNEIDNIEKLLEQVLAKSETIEVLVIDDNSPDGTA
                     LRVKFMQSSEPRIHILERPGKMGLGSAYVTGFKYALERDYDYIIEMDADFSHNPEDIP
                     LLLNAAKKYDLVIGSRYCEGVNIIHWPIKRLLISYFASKYVRTITRMPVKDPTSGFKC
                     FQRKVLENIDLDKILSDGYAFQIEMNFRAWVKGFHIKEIPIVFTERKNGVSKMSRRIV
                     WEAAWMVWRLEFMRILGLLK"
     gene            13366..14382
                     /gene="ruvB"
                     /locus_tag="PLW08_03710"
     CDS             13366..14382
                     /gene="ruvB"
                     /locus_tag="PLW08_03710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPL51741.1"
                     /translation="MLERINNPDKLNEDVELDRALRPRTLKEFIGQTHIKELLDISIK
                     AAKLRGESLDHILLYGPPGLGKTTLAGIIAREMGVNITVSSGPVIEKPSDLAGILTNL
                     QRHETLFIDEIHRLSHIIEEYIYPAMEDFEMEIILDSGPNSRTLKIPLEHFTLVGATT
                     RAGLLTPPLRDRFGIILRLDYYDQESIAQIIRRSARLLNIPTEDDGVQEIARRSRGTP
                     RIANRLLRRVRDYAQIKGEGIITLDIALSALQMLQVDNAGLDEMDKRILTTIIENYNG
                     GPVGIKTIATAIGEDSGTIEEIFEPYLVQQGFLERSPQGRKVTFKAYKHLGLNPASAQ
                     TEIF"
     gene            14392..15819
                     /locus_tag="PLW08_03715"
     CDS             14392..15819
                     /locus_tag="PLW08_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPL51742.1"
                     /translation="MHTKTVISGIRWTLSTTILRRAISLILFIFLAKWLTQTDFGIYR
                     SFSAILALLTYVTHLGLDYVYLVSRQKERVNFLALLQTGLLVSAIITVLLAVFGKNIG
                     AYYNSAELGKVLSYGGGLVFIESLRRIVRVYAQKKFQFKDLALAETINVIIYSVLCLA
                     LIYFWRYAWLFILLFYIGNLAELVYLCVKLPRLPLSLLKRVFSFKWLKKTLALFSRNG
                     NFLLMVNAINLLQAYSINAPILFLGTLVEPALMGVYFFASQLIAIPVGMLNASFAQVF
                     FPVLAQSEKSASVAGIRHYTSLVLKIGIPALIVYALLLQYLVPVIWGDKWLAAMPLIL
                     YLVIYYGTSMLHNPISGIPFICRKPQWELLWNIITLGLRILVLYLGLQVSFAFAILLF
                     CLVSAVMHFAFYYMSLALLKADLWQITIDILTYLPVLLVLALGCLYMGKLSIVFPLVV
                     LIGYGIYLWIAERDTLKEVLSLIKK"
     gene            complement(15961..16674)
                     /locus_tag="PLW08_03720"
     CDS             complement(15961..16674)
                     /locus_tag="PLW08_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPL51743.1"
                     /translation="MNLKELCLFLRELIQDSEFADKSYFAGGCVRDFILNPDATLKGD
                     VDICVELPEGGIALAQYLQNRLQASELVIHKSFGTASICYQNLQLEFVATRKEQYFPN
                     NRYPKVEFGTLVDDVLRRDFTINALLMAIATGEILDLCQKGLNDLQQGIIRTVKDPNL
                     SFTEDPLRMLRALQFALRFNFTIEEKTFKAMRKNAPALKILSHRAIQNELQKIPESQK
                     SEAKQLISNLGWEIILSLF"
     gene            complement(16671..17795)
                     /gene="hemW"
                     /locus_tag="PLW08_03725"
     CDS             complement(16671..17795)
                     /gene="hemW"
                     /locus_tag="PLW08_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPL51744.1"
                     /translation="MEQDFPSSPLGLYIHIPFCLSRCGYCSFFSVPYRNSVLNEYVDY
                     LEKEKALYLNQLSRPLATIYFGGGTPSLQNAEQIRHILSGLNILPEAEITLEINPLQI
                     TPTYLQALKTTPINRLSIGLQSMNQEELNFLERHHKPEQIQEKIALCRQFGYENLSLD
                     LIYGLPNSSLQTLQGTVEKYLELKPEHISCYLLTLDADCKLAKEKNNLPNEEIQAEQY
                     TFLCNYLEKEGYIHYEISNFALPGYTSQHNLRYWHNDDYLALGASSAGWISPIRYQNP
                     ADLNFYYQSVKIGETFPQKEELSQQRIIQDYLMMGLRLKEGIGLDEFYNRFQVNLTEL
                     YGERIFKLQQIGMLNLTEHNLALTEKALFVSNSVIGDLIL"
     gene            complement(17765..18691)
                     /gene="lepB"
                     /locus_tag="PLW08_03730"
     CDS             complement(17765..18691)
                     /gene="lepB"
                     /locus_tag="PLW08_03730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPL51745.1"
                     /translation="MKEKITPKRDKTKSDNLPYTLTPPPEGKKFHKRKPGLQDWMEAI
                     LFAFVVAMIIRNYTFENFLIPSSSMEKTLLVGDYLIANKIKYFFNDPKQGEIVTFRYP
                     KIEEGTPEHKDYKDDFIKIFPPIYINKRHSFAKHPFTAFHICYYARRNVVKRVIGMPG
                     DIVEIKDKIVYVNGKEYTGGFENYGVAIPSPPAAPERIEYFTYPENSFDYVSMNPWYE
                     PYRVKIEGDSSAVRKIFNRDWFGPVKVPEKSYFVMGDNRDVSEDSRYWGFLERKYITG
                     TPWLIFFSRGIEFNKLYDEPHIRWNRIFRHPR"
     gene            complement(18995..20545)
                     /locus_tag="PLW08_03735"
     CDS             complement(18995..20545)
                     /locus_tag="PLW08_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPL51746.1"
                     /translation="MAYVFTPSQMKTLDENTINCFGIPASILMENAGRGCADFLINNY
                     PEETKGVIIILHGYGNNSGDGFVLARWLQNYGKQVILLQVQKGEMTEETRHNFKLCKA
                     LKIPTFELNKEYDNLDILENVPGVTMIIDAVFGTGFKGKLPSSLKKIFQSVDDTSALK
                     VAIDIPSGLNGATGEGEDAFRADVTLTLHSYKMGFFLREGKIKCGKVYTIPIGIPEHY
                     NDAFFTPAIFIDNKTFRLPERYYYANKSNYGRVFIIGGSYGLLGAAALATRAALRAGA
                     GLVYLISREENKYFYNANPAEFLFIGIPRIEGTLTPDLKLFTTLFKTADSVVIGPGLG
                     SDNYGTYMLEYILRTSTVPTVVDADALRIIAKDPSLQQYLKKPNILLTPHLGEFCTLT
                     DMDMDKLVKNIPTALENYIKKTGAKVLLKSDTTIFSDGKRMFINISGNDGLATGGSGD
                     VLAGIIGSFCGQKMELGEAAINASFIMGKTAEELAKQRYFPSILPTDIIENLFMPLKN
                     ESLSSPEG"
     gene            complement(20545..22098)
                     /locus_tag="PLW08_03740"
     CDS             complement(20545..22098)
                     /locus_tag="PLW08_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPL51747.1"
                     /translation="MKPVLKNSILTGKEPKSTNLFNRLRFILIFGSLAIFIFFAVYIQ
                     FLLKQAKKEQEFIPRIFAQYIAYTDRYLREAERNAQLITEISSKYLQFTTSRDFKQDL
                     WDYISTEFMQENPVPVIITDQNFQPLLWKNVGVPTDTIFHELSPENQKKLENLMKTMV
                     QMPLTDDGKISGYAYYSKPVSFAEFIKNVDCSIIVTDHYKQPLFWRNVGIPETANYYE
                     ISTEEQQKLAHQIQTMDEIPLSNAADSLGYIYFSTPKSLSYIRYIVILELILALMVVF
                     FGIYGLFLLGRTEKDTLWVSLAKETAHQFGTPITSLMGWLDYLKEPSPDGCVRDIDQI
                     VQYMTADLEHLKTIASRFGKVGSITKLEPQNLDRIISETVEYFRERMPHLGHKINIHY
                     ISKIEGVMVLMDKDLIKWSLENLIKNCVDAMSQKGGNIIITATHKNPWIYIHIRDEGK
                     GIPHRQWKRIFEPGVTTKTRGWGLGLSLAKRIIEEYHNGHIKVLQSTIGEGTTFEIKL
                     PVQETNKEK"
     gene            complement(22043..22810)
                     /gene="mazG"
                     /locus_tag="PLW08_03745"
     CDS             complement(22043..22810)
                     /gene="mazG"
                     /locus_tag="PLW08_03745"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPL51748.1"
                     /translation="MNKFEQLVEIVATLRTPAKGCPWDLKQTRESLVPNFIEELYEVV
                     EAIENKDYDALKEELGDLMLHIVMQAQISKEQGLWNIDDVLEEISSKLIRRHPHIFGD
                     LHLTDADEVKQNWERLKKAEKTERKSVLEGIPKSLPALIQAQRTQEKAASVGFDWQDI
                     KPILEKLDEEREELAEALNSNEQDAIKEELGDMIFTLVNLARKLHIDAESALKECIRK
                     FTRRFNAIEEHYLKNGEDINEAGLEELDSHWERTKKH"
     gene            complement(22797..23957)
                     /locus_tag="PLW08_03750"
     CDS             complement(22797..23957)
                     /locus_tag="PLW08_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL51749.1"
                     /translation="MYFLFVACLCVIYALINPDLIVINKINFLPAMFMLAWTSYSFLS
                     YNWSFVKQVSVVHSILILKKAMLFIIVSIFCRHQTVKKYAPWFWFSVILVYIVIALWE
                     MLTWQHLPQSLYYNKTNFVPTGPFYGPNQLAAAFNLLLPFILFLPEIKKSKFWGVISL
                     LCFFTIMGIIVIEGARIAIIACSCLFLFAFIFLYSARMRRLSLFLMALIIVGLVIFAK
                     PLVSFGTEMFTEEISSIGQESKSFTMSSIKIRTRLIPQNLEILAESGFKGVGAGNVEH
                     YMLAGRMYKTGGISNSHNFFLELLTNYGIVFFLGFAYLYMLWLYRLWRAIKMNPENRH
                     YYEMYFFALLMFLPTSILPSSIIWEHHYWILFAIINEISHPDNWMPRQYEQI"
     gene            complement(24074..25261)
                     /locus_tag="PLW08_03755"
     CDS             complement(24074..25261)
                     /locus_tag="PLW08_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPL51750.1"
                     /translation="MNKNELGLLDIILVIAKRKTLVISICLLVAIGAIIYSLVVPQYW
                     ESKATLMPIAESGSLGSLGGGVMDLLGSGLLQTDKYDMAVDFIYIMQSRKFQEEVIRK
                     FNLIPYYKINEPDTLKAMELAVKKLSESTMQIFYDQKTYLVNIIAETKNKELSRQIVQ
                     YHLDSLNKYILHSKMSKGRQKREFLEKQVDSHLQAVYSLSEQIRDFQKKNRSIDITQQ
                     TEAQLELYSDIVAEYMQTEIEHSLALSQYSADSPVVINLAEKLQLLKKKIKSLEKSGS
                     DLVPEYIVQIDKIPDLAMQYAQMMLNLEIEKKIIEYLYPQFELAKLEEVKDLPTFEVY
                     DAPQLAGIRSKPKRALTVVISTVAAFILSCLLALIIESLQGENKEKTQAIKAALFGRK
                     KAS"
     gene            complement(25334..26593)
                     /gene="serS"
                     /locus_tag="PLW08_03760"
     CDS             complement(25334..26593)
                     /gene="serS"
                     /locus_tag="PLW08_03760"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPL51751.1"
                     /translation="MLDIKFIRNNIELVRQAISNKNEKADLDKLLEIDEQRRQLQFAF
                     DNLKAHQNSVSALIAQKKKTGENIENELKAMTKTAEEIKALSAQLSIVNGELEKLLLT
                     IPNIPQEDVPVGKDESANKIVKIWGEPKQFSFTPKDHLELAIQNKLLDLPRGAKISGS
                     GFPLYTGEGARIERALINFMLEFHLQKHHYTELMAPVIVNRKTMTGTGQLPKLEEDMY
                     RIEKDDLFLIPTAEVPVTNIFADEVLSYKDLPQKFVAYTPCFRREAGSYGKETKGLQR
                     LHQFNKVELVHFTEPEKSAEALEQILSDAEDILQAFNLHYRVVALCTSDLSFASQKTY
                     DLEVWAPGTGKYLEVSSVSTFGEFQARRANIRYKDKTGKVKHLHTLNGSGVATPRLLI
                     AIWETYQQEDGTIKLPAVLDHFLGITI"
     gene            complement(26587..27369)
                     /gene="tpiA"
                     /locus_tag="PLW08_03765"
     CDS             complement(26587..27369)
                     /gene="tpiA"
                     /locus_tag="PLW08_03765"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPL51752.1"
                     /translation="MRKIYIAGNWKMNKGLQETKNFLETVIPELNKLKLDNVIPLIAP
                     AYPFLEEALKGSYGSCMQVSAQDVSVHSEGAYTGEVSAGMLASLGLKFCIVGHSERRQ
                     YHNETDATVNAKILKLFENHITPLVCIGETLQQREEGKTENVILSQLSGCLKNIKLVS
                     GEEIILAYEPVWAIGTGKTATPFQAQEVHNLIRNWLKSNYSDKVAEKLSLLYGGSIKP
                     GNLKELLDCEDIDGGLIGGASLKEEDFLEMVQIGMKSKGQEC"
BASE COUNT         8126 a         5170 c         5428 g         8724 t
ORIGIN      
        1 agtattttag tttataaaat cccgaaaatc cgaataatcc tgttcatccc agacctattt
       61 tatcttttac gaggggctta cgcacccatc gctattattg tgttgcccta aggggctttt
      121 tgttatgaat gtttaataga tttcttcgga ttaaaaatat atttttgtgg gtattctata
      181 tattactgat ttatcaattc tgatattata ttaggtagtt ttcatctggg ggaagaggat
      241 aacttcctta atggaatcgt tttcggttag caacataact aagcggtcta taccgattcc
      301 ctgtcccccc ataggaggca ttccgtattc cagtgcttct aaaaaatcct catctacgac
      361 attggcatcg tcaattccca aagcacgtaa ctttgcctgt tcttccaaac ggactctttg
      421 ttccaaagga tcattcaatt cactgaaggc attggcaaat tcgccgccgg caataaaaag
      481 ttcaaaacgg tctgcaacaa agggattttc gggttttgcc ttagccaaag gagagacctc
      541 tttgggaaaa tcacgcacaa aagttggttg gataagtgtt tcttctacaa aattttcaaa
      601 gagcagagca atcagttttc ctttttcgga gcccggagga ataaccaact ccttgttttt
      661 acagatggca gaaagctctt caaagctgat attcattaca ttctgtccga caaattcact
      721 aaccaaatcg ggcaaagatg ctcttttcca ttctccgctt aagcaaacct ggtgattatg
      781 ataaaagaaa gtatccttgc cgattatgtc caaagccaaa tgtttaatca gctcttcagc
      841 gatatccatc atcccttcca ggtcggaata tgcttcgtaa aactcaatca tcgtaaattc
      901 cggattatga gttctatcca taccttcatt gcgaaaatcc ttgccgagct cgtaaacttt
      961 ttcaaagccg cctactatta aacgctttaa ataaagctcc gtagcaattc tgagatacaa
     1021 atccatatcc aaagtgttat gatgagtaat gaaaggtctg gcatttgctc caccgtaaag
     1081 gggttgtaaa ataggagttt ccacttccac ataaccccgg ctatccataa aattccgaat
     1141 agcggtaatg atttttgcac gggtttcaaa aacttttcgg tgttcaggat tgagcaaaag
     1201 gtcaagatag cgttttctat aacggagttc aatatcggaa aattcatcgt aacggatttt
     1261 tttaccgtcc acgatttttt ccttcactgc aggtaagggt ctgatgtttt tggaaaggag
     1321 ttttatatta ttgcatttta gggaatactc acctgtttgc gtgaaaaatg tttttcctgt
     1381 ggcttgaata aaatcgcccg gatcacaaag tttgaatagt tcgtagtttt cctcgccgat
     1441 taaattttga gctacataaa gctgaatttt tccggaaata tcttccagat cgccaaaacc
     1501 gattttaccc tgccgtcgca tagctgttaa acgcccggtt atagtgactt cttcagcttt
     1561 ttgaagctgt tcttccttat ctttaagcag gtcagaaata taatgagtac gataggattt
     1621 tatgggataa ggatcaatac ccagggcttt aatttttgcc agtttttctt tacgcaactg
     1681 gataaactga ttgggtttgc ttgacattac ggaaaattaa aggtctttga aaggattatc
     1741 agctgccgga tttactttct gattggcata cttacgccac tcctcatcac tgcgatttct
     1801 tccaccccgg cgtgggtttg aactacgaat tttttcggta tgctgatctc tatagttatg
     1861 ttcttccgaa cggacgcgtt cttcagccaa ttctacaggt gaacgatcgg tataatcaag
     1921 agtaattcta tgattttctt tatctatggc agttaccatc aaagttaccg gctcgcctgt
     1981 ttggaaggta tcatttttcc gtacccggga tttgggcaac agacctgtaa ttccttcact
     2041 gatagtaaca aaaacgccga aattggtaga gctttccaca attccttcaa aaggagtttc
     2101 cagctgaata atttcatcta ttttatccca ggggtctgct tccaatgcct tcagcgaaag
     2161 agatatctta tgagtatctt catctatgcg taaaatttga acttgaaccc agtccccttc
     2221 tttcacaata tcccggggat gagaaatgtt gcgattacgg ctcatttcag aaataggaat
     2281 aagaccttca acaccgggtt tcaattcggc aaaagctcca aaattatgta aacggagaac
     2341 acgacaattg atgatatctc cttcatgcag ttctttcatt gctacttcaa aggggtcttc
     2401 aagcaagacc ttacgcgaaa gagcaatttt ttctcccttg atatccataa ttttaacctc
     2461 aatttcttga ccttttttca gttcgtcttg cggtctaaca atatgctgcc aggaaatttc
     2521 cgaaacatgc attaaacctt caattccgcc caagtcaata aaagctccga aagtagtcat
     2581 tcttaagacc ttacctttaa cgatatcacc cactttcaat ttggctaaag ccatttcttt
     2641 tctttcctgt tctcgcattt cttccagttg acgatgcgaa acaactatcc gcctgcaatt
     2701 ttcggaacat tcaataacca gaaaatcaag ggttttacct ataaactgag aactatcttc
     2761 cacaggatgc aatgagacct gtgatacggg acaaaatgcc cgtgctccaa gaatatctac
     2821 attgaaacca cccttcgttg gagtatatat ttttccctgc accgggattt ttttctcaaa
     2881 tgcctcaaga atggattgtt tatctacata ctgtttagtc aagctccggg caacgacata
     2941 gccattttca ttctggtcaa caacataacc ctttaagttg tcacctactt tgtaaggcaa
     3001 aactcctctg gaatcggaaa actctccgat ttcggcataa gcatcaaatt taccacctaa
     3061 attaaggatt atgtattggt cgccgatgct tacaatggga gcttcaataa catcgccctt
     3121 tttaatttct gctgtgtttt gaaaggattc ttcaagcatc cgcagatatt cttctttcat
     3181 ttctttcata gcagctttgc tgcttgtagt atgtaacttg tctgacataa gattctccta
     3241 aaggcatttc agagaaacca agatttagag tggctgtttt ttgtaaagat ttttatttat
     3301 tcagctttga acaggagata aaaagaagct gagaattacg attttcagtg atggtttttc
     3361 aaactgtaaa ccagttttac cttttttgtt tgacatttct tgccttataa aaatctgttg
     3421 cctgtaaaaa tgagaaaata tactgtttat atatcttaca atattcacag ataaaggact
     3481 gacaatgaaa aatacttacc cggaattgga caaaattttg gctaaatatc aggacaagaa
     3541 aggtgctctg ataccccttc tgcaagaagt ccaaaaagaa aagggttatt tatccagaga
     3601 aacaatgcaa tatttagcgg ataaaatgaa aatacctcct gcggaaatat ttggtgtagc
     3661 caccttttat tctatgtttc gcttaaaacc gcagggtaaa catcttattc gtgtttgtaa
     3721 aggaactgcc tgtcatgttt ccgatgcgga tggcataaaa aaagccgtaa tggatgcatt
     3781 acagctttcc gaagaagaaa atacaactgc cgatatgcaa tttaccgtaa tggaagttgc
     3841 ctgcctgggt tgttgttctt tatctccagt aattatgata gacggaagaa cttatggtaa
     3901 attaacaccc ggggcaattc ctgctgttct gaaacaatat gaaatagagg atactttatg
     3961 aataacatca tcgtgaaagt tggtttagca agttgtggtg ttgcagcagg tgccaaagaa
     4021 gtatatactg ctctggaaca atatcttgaa aataatccga gacctgtaat tctaaaaaag
     4081 acagcttgca ttggaatgtg ttttgaggag ccaattgtgc aatttgaggg ttctgaactt
     4141 ggttccattc atatcggtaa agctaacccg gaaactataa ctcatatact ggaggactat
     4201 atagcaggaa aaactcctgc tgaaaacata attttagcgg aaaagattag tggtaagcat
     4261 aatgctcttt tggcaaatca gcaaagaata gttttgcgca attgtggaat tatagacccg
     4321 gaatgtattg aggattatga agccggtgat ggctataaag cactgcaaaa agcactgcaa
     4381 atggaaagaa cagccatcat tgaagaaata aaaaaatctg gtttaagagg aagaggcgga
     4441 gccggttttc ctacaggttt aaaatggcaa ttttgtgcct ctgaaaattc ggataagaaa
     4501 tatattattt gtaatgcgga tgaaggagac cccggtgctt ttatggaccg cagtactttg
     4561 gaaggtgacc cccattccgt aattgaaggt atgattattg gtgcctatgc tattggtgca
     4621 aatgagggtt atatttactg ccgggcagaa tatcctatgg ctttggaaca tttgaaaact
     4681 gcaatttctg ctgctgaaga aaagggttat ctggggaaaa acattctggg tagctctttc
     4741 agttttgatt tgcatattaa agaaggtgca ggtgctttcg tttgcggaga agaaactgct
     4801 ttaatgcaat ctattgaagg aaaaagaggt atgcctactt tacgccctcc ttatcctgca
     4861 gttaaaggac tttggggcaa accaacaaac atcaataatg tagagacctg ggcaaatatt
     4921 ccctggataa ttatgcacgg tgcacaggaa tttagaaaat acggaacgga aaaatcaccc
     4981 ggaactaagg tctttgcttt agcgggaaaa attgctggta gcggtttaat tgaagttcca
     5041 atgggaattc ccctgaagga tattatctat aaggttggag gaggaatgaa gacctccaaa
     5101 ccctttaaag cagtccaaat gggaggtcct tccgggggtt gcattcctgc tgaaaaactt
     5161 gacaccatag ttgattacga ttctattact gcaacaggtg caattatggg ttccggaggt
     5221 atggtagtaa tggattcagg aacctgtatg gttgatgtag ccaggttttt cctgaatttt
     5281 acccaaaatg aatcctgcgg aaaatgcact ttttgccgaa ttggcacaaa acggatgctg
     5341 gaaattttaa cccgcattac agaaggtaaa ggcgaattgc aagacataga aaaactggaa
     5401 gaactggcag taaatattat caagggctct ctttgtggct tgggacaaac tgctcctaat
     5461 cctgttttaa ctactttacg ctattttaag gatgaatatt tggctcatat tgtagataaa
     5521 cactgtcccg cgggtgtttg caatgctcta ataacttata aaatcaatcc cgaaaaatgt
     5581 atcggttgca ctctttgtgc cagaaaatgt cctgtttcct gtatctccgg acaagtaaaa
     5641 caagttcata ctatagacca aagtaagtgt actaaatgtg gaacctgttt ccaagtatgt
     5701 aagtttgatg cggtaactaa agaataggag tttaggcaat gataaatgta agtttaaatg
     5761 gaaaaccagt gaaggtagag cccggaatta ctattttgga actctgcaaa cagaataaga
     5821 tagatattcc tactttatgt aacgatgaag aattaaatcc ttatggttcc tgctgggtct
     5881 gcttggtaga agtgaaaggc cgaaagggtt ttgtaacttc ttgcggaaca actgtttctg
     5941 aaggaatgga aattataacc gatagcgagt caatccataa agcgcgtaaa atggctttgg
     6001 aattacttat ttccaatcac tatgcggatt gtgttgctcc ctgcacagtt gcctgccctg
     6061 attttgttga tattcagacc tatgtttctt tgatagctaa cggaaaatat catgaggcag
     6121 tgaaagttat taaagagaaa ctgccaatgc cattaaccct tggtcgtatt tgtcctgcct
     6181 tttgtgaaaa ggaatgcaga agacaaattg tggatgaaag cattgctatt cgtcagctga
     6241 aaagatatgc tgccgatatg gataacgaag atgtgtggaa ttatgttccc gaaaggttac
     6301 cttctaaaaa caaaagagtt gctattatcg gagcaggacc cagcggacta acctgtggct
     6361 attatctttc caactggggt tatgaagtta ttatttatga agcagctccg aaagcaggtg
     6421 gttggttgcg ttatggaatt ccggaatttc ggcttcccaa agatgttttg gatgcggaaa
     6481 tagagctgat gtgcacgaat ggaatgcaga ttgaatataa taaacaattg gggaaagata
     6541 tctttttgca agacctggca caggattttg ctgccgttta tcttgcaata ggagcacaaa
     6601 aagcagttcc aatgccggtt aaaggcagtg atttagaagg ttgcttctta ggggttgatt
     6661 ttttgaaagc gcacgcttta ggcaattcac ctgatttagg attaaaagta gctatcgtcg
     6721 gaggaggaaa tactgctgtt gattgtgccc gaactgccgt tagaaaaggt tgtgaggttt
     6781 cagttattta cagaagaact aaagctgaaa tgcctgccga gccaagtgaa attgccgctt
     6841 gtgaaaaaga aggcgttaaa ttcttctatc ttgccaatcc tgttgaatat ataggcaagg
     6901 gtggacatct aaccagtgta aaaatagaaa aaatgcgttt aggagagccc gataaaagcg
     6961 gaagaaaaag acccgaacct accggtgaat attttgaact gccttttgac agcatcattg
     7021 ctgctatttc ccaagttccg gaagtggata tcttcactca agagaataat tatgtagcag
     7081 ataaaatatt gcctgtttcg cgttggcaga ccgctattgt agatgaatca actatgttta
     7141 ctggcttggg taatattttt gccggaggtg attttagaag aggtgctgct actgcaattg
     7201 aagccatagc cgatggcaga atagctgcaa ccgctataaa caggtttttg gaaacgggag
     7261 aaataaccgc agagctgatg ccttttgaca gtaaaaaagc taatacatta caagaagtta
     7321 gcccggaaga gtattctata tatgaaaaaa ttgagcgcca agagatgcca gaactgcctg
     7381 taattgaggc aaaaaatagt tttaaagaag tggagctcgg tttttctgaa gagacggcaa
     7441 aggcagaagc aaaacgttgt ttggaatgtg gttgttttgt aaatgaaacc tgtttcctgc
     7501 gtaaatattg cacagattat gtagttaatc ctgctcaatt tttaggggat aaaaataaac
     7561 atccaattga ttacagtcat ccttttatta tgcgggatgc caataagtgc attaattgcg
     7621 gacgctgtat cagaacctgt gccgaaattc aaggtcctgc agttttgggc tatatttata
     7681 gaggttttgc cactcttgta gctcccgaat ttggggattc gttaaccaaa acaagctgtt
     7741 tatcttgtgg aaaatgtatt gatgtttgtc ctgtgggtgc tttaatgcaa aggactgttc
     7801 actataaatt caatcctcat cctaaagata tctctctgca aaattgtggt ttatgtggag
     7861 tgggctgttt tattcaaacc gaaacacaag caggaattgt tacccgggtt acaactcccc
     7921 aagaggaacc tgcttttaac ggtaaaaatc tctgttttaa gggaagattt ggctggcaga
     7981 gcttttatga taaagataga ttgaaagttc cgctgtttaa ggaaaacggc atttttaagg
     8041 aaattagctg gcaggaagca actgatttaa tagccaaaga aatttcctta tgcagttcaa
     8101 aacgctttga aattagtcct tatatttctc tggaagaaat gctgatgctg caaagagctg
     8161 ctcataaata tgagacagaa ctttctgcca atcctgtttt ttccgccttc agcgatgttt
     8221 ttctaaactt aagtccgcaa aaagaaccct ataaaattct ggatgacttt gaagaatatg
     8281 ttgtttacgg agaactaaat caggtgctgg caactttaat ccgcttacag caaagaaagg
     8341 gaaagaaatt aactttagta aactatccgg aaaatgcgtt ttgtcgtttt gctgatgctg
     8401 tttatgccaa tttggcagaa gttcaggcaa cggataagac actctttatc tataatcaaa
     8461 atcgcatttc ggaacaggaa gcttttgcct tatggtgttt tgcctctgaa caaggaactg
     8521 ataatcttct ggtttctaca gattttcgta atcatcttgg atttttagct atgcagccgg
     8581 atttctctcc ttccagttcg gattttgttt taagttgggg tgtttatccc gctttagcca
     8641 atcaagcaaa atttagtgtt gccataatgc cttttgagga tgaaaaagca cctgtggatt
     8701 tgttaattcc ggctccgagt tttctggaaa tggaaggaac cgctttaagt gatttggggc
     8761 agataacaaa aagtgctaat cctgccaact caattattat caatgaattg atgcgtttgt
     8821 tttataccct gaattggatt catcccaata gtgcagaagt gcctttttgg aaccaggaag
     8881 cagaaaaatt gttgttaacc cttcagaatt atgttcctgc caagtttgag ccatcagctg
     8941 ttaaaattgc tgaattaagc aaaactatca aatttattcc tgctcaagca gaaaagtata
     9001 tcactgcgct ttttgagcaa ggtaaaaagg caacaagctt ttccggaagg ttgatttcct
     9061 cgtcaactaa tatgtaaatc aggagttgtt tgcgccttaa tattttttct tgggttatat
     9121 ctttcaaggt gcaaagggct tatgacgatt agaacaatta tatattatta actgcatagc
     9181 cggaacttgc tgcagctctg cattatttta cgatttttat taggtcctca tttttccact
     9241 ttttgcggag ggacggtgtc ttccagctac atagatatca gttataacag catttctttt
     9301 agctttggat agagaatgca attcatagcc aagttcttgg tgtttttgtt gaagagctgt
     9361 taaaatatcc gtagcagatt gtgtttgcag catttcctgc cacatacttt tgtgttcttc
     9421 tttattccaa tacaggttta agagcttggc aaaattctcc agttgttgta tttcctcaaa
     9481 agtaagggca tcggaagaaa ggcattgata gggtggttgg ctgagccatt tataattcaa
     9541 ttccgaggca attttctgca ttggggtatc aggtaaaact tttaaaatgc caagttgaac
     9601 agcatccgga agagtagcgc agacctcatt taagccattt aatacggaag cataattttc
     9661 cccgggcaaa ccggcaagca agtcagtatg gatgcgtata ttagttcgtt ttttcagttc
     9721 ttgcaaagtc cattttgtct tttcccaatt agattttctg ccgcaattca aagctacttc
     9781 ggagtttatg gtctgaatac caatttccaa acggattcta ttttccggtg ctttttccag
     9841 taaagcaata tcatcttctt ccaacaaatc agggtagatt tcaaaatgaa catcgcaagg
     9901 gcaattttgt ttcataaaga acttccaaat atagtgtgcc agctgtttat ggagattaaa
     9961 actgcggtct acgaatttta tagttcttgg ctgaaaagaa atcaaggtct ccaattcctt
    10021 attcaggcat tgcatatctt tttggttatt tggctgatag cgaagttcgg agcgtttatc
    10081 atttgccgaa agacaataaa tgcaggcaaa agggcagccc ctaaatgttt cataatatag
    10141 caatttgccc tttaaaatct ccccatcgct ttctctgtaa gggaaaggaa tatctttcaa
    10201 gggaatatgc ttagcccgag aaaggtcttc atcattagca gaaaaatcgt gttctgccag
    10261 aaagcgaaat tttccttcac cggcactttg gacaatataa agtccttttc ttttcaggaa
    10321 ggataaactt tccgcttcgg gaccgccaat tacaataact gcttcaggta ataacttgat
    10381 aacttcagga atcaattcct gcagataaag acggttccaa atgtaagcag aaaaacaaat
    10441 tacctctgcc ttgtggcaat aaatttcaga cagcacattc cagagccggt cttttaatgt
    10501 ccattcctgg agaataacag cataaggcaa atctgcaatc atttctcgta agtaatagat
    10561 tgcaggattg gattgactcc aactgctgtt tatggctgtc agaagaatct ttttcatata
    10621 tttttctctt ttttacacaa aggacaccgt gaacacctag aagataattt taagatattt
    10681 attataccgc taattgcgcc tgaatttaat ccggtagctc caatggctcc agtcagttgc
    10741 gtgctataat ttctaactta aatcttcaag aaaaatctgc tttttctcct tgttttcagc
    10801 cctatatcgc atttaaccat cccaattgat tcccatattg cgatatggga acgatatggg
    10861 aatgatatgg gaagcataag ggataggatt atggattttt taagcgggtt cattccttgc
    10921 ttctgggatg ccctttttta tatgcttcct ttatatcttc cagggaaatg tgggtatagg
    10981 tctctgtagt agagagcaga gagtgtccta aaagttcctg gatggctctt aaatctgccc
    11041 ctctggaaag caaatgggtg gcaaaactgt gtcttaaaga atgaggggaa tagcccttag
    11101 ctttggcaac gagttcaaaa tagcgtttta gaataatatc cagctgtttt gtgtcaaatg
    11161 ctttaccgct tttagttaaa aagagcaaat cggggttatt ttcccgcaca aattggggac
    11221 gcacctttaa atagttattt attgcttcaa tagcataaga accaaggggc acaatacgct
    11281 gtttattgcc ttttcctgta acccttacca atcctctttt caaatcaata tcctgcaaac
    11341 ggattcctgc aagttcggaa atacgtaatc cggaagaata caaggtctcc aaaattgcct
    11401 tattccttat tccgaaggga ctatcggtat ccggaataga aagtaaagtg cgcacttctt
    11461 cttcggtaaa aaatttggga agaggaactt cgtatttggg acgcttaatt ttttccatag
    11521 ggttattctg aataaaaccg cttcttttac aatagcggaa aaaggaattt aaggcagcac
    11581 ttttacgcgc taaagagcgg ttacaatccg gtttttcatt aagaaagcgg aaaaaatccc
    11641 gaatattaag aacggtaatt ccttttatat ccacttcgct attttcaaaa tagcgttgaa
    11701 taaatgaatg gaattgttcc aaatccagct tataggcagt aatcgttctg ggcgatttac
    11761 cttccaaagc aagataattg cagaaatcag ctatatattt ttccataaat ccattttttt
    11821 tcttgcctta aaacttatca agcaaaaaat ggtattaagt aaaaaaaggg aggacgaatg
    11881 aaccggttta ttgacgaaag tgctaaaatt ggaatgaatg taaccttagg caataatgtc
    11941 gttattatgg caggagtcca gattggaaat gactgtctga tagggcataa tgttattatt
    12001 catccggata caaaaatcgg aaatgcctgc cggatagatg atggaaccat aattggcaaa
    12061 aaaccgcttt cctctccacg cagtattttt acagtgccaa cagacctgaa aggcacagaa
    12121 ataggtgatt tttgccagat tggcagtaat gtaattattt attgtcaatg cacaatcggt
    12181 aaccgtaatt taatcgctga tctggcaact atacgggaaa atgttacttt aggcgattta
    12241 aacatagtcg gcagaaatgt tactattgag aactttgtgc atataggcaa cagaaataag
    12301 ctggaaacaa actgctatgt tactgcctat tcggaaattg gtgactattg ttttatagct
    12361 ccctgtgtag caacaagtaa cgataattat atgggtcgcg ataaagaacg ctttaaacat
    12421 ttcaaaggag taacaatgat gactggttcc aggataggag ttaacgctac aatcctgcca
    12481 ggaaagacaa ttcatagtga tggaactgtt gcaggtggag cagttgttac caaagatgtt
    12541 cccgctaaaa taatcgtggc aggaaatccg gcaaagccat ttagcgaagt tcccgaaccc
    12601 caattgctgg aaaataatct ggataaacaa tgaaaacact aataattata ccaacttata
    12661 acgaaataga caatattgaa aagctcttgg aacaggtttt agccaaaagc gaaacaattg
    12721 aagtgctggt gattgatgat aactctccag atggcactgc tttaagagtt aaatttatgc
    12781 aaagttccga accccgcatt catatactgg agcgtccagg aaaaatgggt cttggttctg
    12841 cttatgttac aggttttaag tatgctttgg aaagggacta tgattacatt atagagatgg
    12901 atgccgattt ttctcataat cctgaagata ttcctctgtt gctaaatgca gccaaaaaat
    12961 acgatttagt aattggtagt cgctattgtg aaggtgtaaa tattattcac tggcctatca
    13021 aaagattgct aatcagttac tttgccagta aatatgtccg cacaattacc aggatgcctg
    13081 ttaaggaccc aaccagcgga ttcaaatgtt ttcagcgtaa agttctggaa aatattgatt
    13141 tggataaaat cctctccgat ggatatgcct ttcagataga aatgaatttt agagcttggg
    13201 tgaaaggttt tcacatcaaa gaaatcccta ttgtctttac ggaacgcaaa aacggcgtct
    13261 ctaaaatgag ccggagaatt gtttgggaag ctgcctggat ggtttggcga ctggaattta
    13321 tgagaatttt gggattgctg aaatagcgaa aaaaacaggt aactaatgct ggaaagaatc
    13381 aataatcccg ataaattgaa tgaagatgta gagctggata gagccctacg tcccagaacc
    13441 ttaaaggaat ttatcgggca aactcatatc aaggaacttt tggatatcag tattaaggca
    13501 gccaaactgc gtggggaatc gcttgatcat atccttttat acggacctcc tggtttggga
    13561 aaaacaaccc tggcaggaat tattgctcgc gaaatgggag ttaatataac tgtttccagt
    13621 ggtccggtta ttgaaaaacc ctcggatttg gcaggcattt taactaatct ccagcgtcac
    13681 gaaacactgt ttatagatga aattcatcgt ctctcccata ttattgaaga atatatctat
    13741 cccgctatgg aggattttga aatggaaatc atcctggata gcggtcccaa ttcccgcacc
    13801 ttaaaaattc ctctggaaca ttttactctt gttggagcta ctaccagagc gggtttgctt
    13861 actcctcctt tgcgtgatag atttggtatt attctgcgtt tggattatta cgatcaggag
    13921 tcaattgccc aaattattcg ccgaagtgca cgcttattaa atattcctac ggaagatgat
    13981 ggcgttcaag aaattgccag acgtagtaga ggcactcctc gtattgctaa tcgtttactt
    14041 agaagagtgc gtgattatgc tcaaataaaa ggggaaggaa ttataacttt agatattgct
    14101 ttgtctgctt tacaaatgct gcaggtggat aatgccggct tggatgaaat ggataaacgc
    14161 attttaacga cgattattga aaattataat ggaggtcctg taggcattaa aacaatcgct
    14221 actgccattg gagaagattc cggaaccata gaagaaattt ttgaacccta tttggtgcag
    14281 cagggtttcc tggaacgaag tccccagggc agaaaagtta ctttcaaagc atacaagcac
    14341 ttaggtttaa atcctgcctc cgcgcagaca gagatttttt aacagaggca aatgcacaca
    14401 aaaacagtga tttcaggcat tcgctggact ctttctacaa ctattttgag aagggctatc
    14461 agtttaatcc tttttatctt tctggcaaaa tggctaactc aaactgattt tggcatttac
    14521 cgcagttttt ctgctatttt ggcattgctt acttatgtta ctcatttggg gctggattat
    14581 gtgtatcttg tttccagaca aaaagaaagg gtaaatttcc tggctctttt gcagacggga
    14641 cttcttgttt ctgctattat aactgtttta ttggctgttt ttggtaaaaa catcggtgcc
    14701 tattataata gtgcagaact gggcaaagtt ctttcttatg gcgggggttt ggtttttatt
    14761 gaatctctgc gtaggattgt gcgtgtttat gcgcaaaaga agtttcagtt taaagacctg
    14821 gctttggcag aaacgatcaa tgttatcatc tattctgttc tttgccttgc acttatctat
    14881 ttttggcgtt atgcttggtt gtttattttg ctattttata taggcaatct ggctgaatta
    14941 gtttatctgt gtgttaaact accacgcttg ccactatctt tactgaaaag ggtttttagt
    15001 tttaagtggc tgaaaaagac gcttgcactt ttttcccgta acggtaattt cctgcttatg
    15061 gttaatgcca taaatctttt gcaggcatat tctatcaatg ctccgattct ttttttgggg
    15121 actttggtgg aaccggcttt aatgggtgtt tatttctttg cttcacaatt gattgctatt
    15181 cctgtaggaa tgttgaatgc ttcttttgct caggttttct ttccggtttt agctcagagt
    15241 gaaaagtccg caagtgttgc aggaataaga cattatactt cactggtttt aaaaataggc
    15301 attcctgctt tgattgttta tgctcttctt ttacagtatc ttgttcctgt aatttggggt
    15361 gataaatggc tggcggcaat gccgttaatt ctttatttgg taatttatta tggaaccagt
    15421 atgctgcata atccaattag tggaatacca tttatttgcc gcaaaccgca atgggaattg
    15481 ctatggaata taattactct tggtttgcgt attttagtgc tgtatttggg tttgcaggta
    15541 agttttgcct ttgctatcct gctgttttgt ttagttagtg cagttatgca ctttgctttt
    15601 tactatatgt ccttagcttt attaaaagca gatttatggc agataactat tgatatatta
    15661 acttatcttc ctgtgttgct tgtgcttgcg ttgggatgcc tgtatatggg gaaattatct
    15721 attgtttttc cgctggtggt gcttataggt tacggaatat atctttggat agcagaaagg
    15781 gatacactga aagaagtgct gtctctgata aaaaaataac ccgagtttat ttacacttag
    15841 aacaccgaaa acactaagaa tactgagaag aatagctttt tatatttctt gaatgaaaag
    15901 ccattgagca tccttgcaaa agcagaaatc cagatcagtt ttaataatat tacggtaaat
    15961 tcaaaataga gacaatatta tttcccaacc aaggttacta atcagctgtt tagcttcgct
    16021 tttttggctt tccggaattt tttgtagttc gttttgaata gctctatggg aaagaatttt
    16081 taatgcagga gcgtttttcc gcattgcctt aaaggttttt tcttcaatag taaaattaaa
    16141 gcgcaaggca aattgcaggg ctctaagcat tcttaaaggg tcttccgtaa agcttaagtt
    16201 cgggtctttt acggtgcgga taattccctg ttgcaaatcg ttcagtcctt tttggcataa
    16261 atccaaaatt tcacctgtgg caatagccat cagcaaagca ttgatagtaa aatctcttct
    16321 gagaacatca tctactaaag ttccaaattc cactttcgga tagcggttat tggggaaata
    16381 ttgttctttg cgtgtagcta caaattccag ttgcaggttt tgatagcaga tagaggcagt
    16441 tccgaaactt ttatgaatca ctaattcact tgcttgcaat cggttctgca aatattgggc
    16501 aagggcaatt cctccttcag gaagttccac acagatatct acatcacctt ttagggttgc
    16561 atcagggttt aagataaagt ccctaacgca tccaccggca aaataacttt tatcagcaaa
    16621 ttcactgtcc tgaattagct ctctgagaaa taaacataat tccttcaagt tcatagaatt
    16681 aagtccccga taacgctgtt ggaaacaaat aatgcttttt cggttaatgc caaattatgc
    16741 tctgttaaat tcagcattcc tatctgttgc agtttaaata tcctttctcc atatagttcg
    16801 gtaaggttaa cttggaaacg gttgtaaaat tcatccagac caataccttc ttttaaacgc
    16861 aaacccatca taagatagtc ctgaattatt ctttgctggg aaagttcttc tttctgtggg
    16921 aatgtttcac caatttttac gctttgataa tagaaattca ggtctgcagg attttgataa
    16981 cgaataggag aaatccaacc tgcagaagag gcacctaaag ctaagtaatc atcattatgc
    17041 caatatctta agttgtgttg agaggtataa cccggaaggg caaaattgga aatttcatag
    17101 tgtatatagc cctctttttc caaatagtta caaagaaaag tatattgctc tgcctgtatt
    17161 tcttcattgg gcaaattatt tttttccttt gccagtttac aatccgcatc caaagttaaa
    17221 aggtagcagg aaatatgctc cggtttcagt tccaagtatt tttccactgt cccttgcaag
    17281 gtctgaagag aagaattggg caaaccgtaa atcaggtcta aggaaagatt ttcatagcca
    17341 aactgacggc agagagctat tttttcctga atttgttcgg gtttatgatg tctttccaga
    17401 aaattcagtt cttcctgatt catactttgg agtccgatag ataaccgatt gataggtgtt
    17461 gttttaagtg cttgcagata ggtaggggtt atttgcaaag gattgatttc caaagtaatt
    17521 tccgcttcgg gaagaatatt cagtccgctt aaaatatgcc taatttgctc ggcattttgc
    17581 aaagaaggag ttccaccgcc aaagtaaatt gtggctaaag gacggcttaa ttgattaagg
    17641 taaagggctt tttccttttc cagatagtca acatattcat ttaaaacact gttacgataa
    17701 ggtacactga aaaaactgca atatccgcaa cggctaagac aaaaaggaat atggatatac
    17761 agtcctaacg gggatgacgg aaaatcctgt tccatcggat atgaggttcg tcatagagtt
    17821 tgttaaattc aatacctcta ctgaagaaaa tcagccaggg agttccagta atgtattttc
    17881 gttccaggaa accccaataa cggctatctt cactaacatc acgattatca cccatcacaa
    17941 agtaactctt ttccggaact ttgacaggac caaaccagtc acggttaaat attttccgca
    18001 cagctgaaga atcaccttca atttttactc tgtagggttc ataccaaggg ttcattgaaa
    18061 cataatcaaa gctgttttcc ggataggtaa aatattcaat tcgttccgga gcagcaggtg
    18121 gactgggaat ggcaacaccg taattttcaa aaccaccggt gtattctttc ccgtttacat
    18181 aaacaatttt gtctttgatt tcaactatgt ctccaggcat accgataact cttttcacta
    18241 catttctgcg ggcataataa caaatatgaa aggcagtgaa aggatgcttg gcaaaactat
    18301 gccttttatt gatatagata gggggaaaga tttttataaa atcatcttta taatctttgt
    18361 gttcaggagt gccttcttct attttaggat agcgaaaagt gactatttca ccctgcttgg
    18421 gatcgttaaa aaaatacttt attttgttgg caatcaagta atcacccact aaaagcgttt
    18481 tttccattga ggaagaaggg atcagaaaat tttcaaaagt gtaattgcgg atgatcattg
    18541 ctaccacaaa agcaaaaaga attgcttcca tccaatcctg caaaccgggt ttgcgtttat
    18601 gaaacttttt tccctccgga ggaggagtta aagtgtaagg caggttgtca gattttgttt
    18661 tgtcacgctt gggagtgatt ttttccttca tcaattagta tcctttttgt tatctgcaat
    18721 ttagttacca acctatgaaa gtgctgtttg tgtcaagcaa aatggtaaaa ttggtttttt
    18781 ggctcacgca gattatgttt acactaaaac aacgaaaaca ctgaggatac tgaaaaaaat
    18841 agcccacaga ttacacagat aacacggact taatagggac aggaatagaa tgtataggaa
    18901 cggataaaac cgtatctttg ggcttattga tagtccatct ttctcagatc aattcaatca
    18961 tttttctttc ttctttctta gtcattactc aacatcaacc ttccggagaa gataaacttt
    19021 cattttttag gggcataaat aggttttcta tgatatccgt gggaaggata gaaggaaaat
    19081 agcgttgttt ggcaagttct tcagctgttt tacccataat aaaagaagca ttgatagctg
    19141 cttctcccag ttccattttt tgtccgcaaa aggaaccgat aataccggct aaaacatctc
    19201 cgctgccgcc tgtagcaagt ccatcattac cactgatatt aataaacatt cttttcccat
    19261 cgctaaagat tgtagtatca cttttcagga ggacttttgc cccggtcttt ttaatataat
    19321 tttccaaggc ggtaggaata tttttaacca gtttatccat atccatatct gtcagggtgc
    19381 agaattctcc caaatgaggt gtcagcagga tattaggttt tttaagatat tgttgcagag
    19441 aagggtcttt ggcaattatg cgtaacgcat cggcatctac aactgtagga actgtagatg
    19501 ttcttaaaat atattccagc atataggtac cataattatc agatcccaag ccgggaccta
    19561 taacaacgct atcggctgtt ttaaaaaggg tagtaaataa tttgagatcg ggagttaatg
    19621 ttccttcaat tctggggatg ccgataaata aaaattccgc aggattggca ttatagaaat
    19681 acttattttc ctcacgactg attaaataga ccaaaccggc tcctgcacgt agggctgctc
    19741 ttgtagccaa agctgcggca cctaataaac cgtaacttcc tccgattata aagaccctgc
    19801 cgtaattgct tttgttagca taataatagc gttcgggcaa gcgaaatgtt ttattatcaa
    19861 taaaaatagc aggagtaaaa aaggcatcgt tataatgctc gggaattccg atggggatag
    19921 tatagacctt tccgcattta atttttcctt cgcgtaaaaa gaaccccatt ttataagaat
    19981 gaagggttaa ggttacatct gcccgaaagg catcttctcc ttctcctgta gctccattta
    20041 gtccggaagg tatatctata gctactttga gagcagaggt atcatcaacg ctttgaaata
    20101 ttttttttag agatgaaggc aatttaccct taaatcccgt tccgaagaca gcatcaataa
    20161 tcattgtaac acctggtaca ttttctaaga tatccaggtt atcatattcc ttgtttaatt
    20221 caaaagtagg aatttttagg gctttacaaa gtttaaaatt gtggcgagtt tcttctgtca
    20281 tttctccttt ctgaacttgt aagagaataa cttgttttcc gtaattttgg agccaacgag
    20341 caaggacaaa gccatctccg ctgttatttc cgtaaccgtg caggataata ataacccctt
    20401 ttgtttcttc tggatagtta ttaatcagaa aatcggcaca gccccttccg gcattttcca
    20461 ttagaataga agccgggatg ccgaaacaat tgatggtatt ttcatccagg gttttcattt
    20521 gtgagggggt aaaaacataa gccattattt ctccttattt gtttcctgaa caggaagttt
    20581 tatttcaaaa gtagtgcctt cgccaattgt gctttgcaga acttttatat gcccgttatg
    20641 gtattcttca ataatgcgtt tagccaagct taaacccaaa ccccaacccc gggttttagt
    20701 agtaactcca ggttcaaaaa tgcgtttcca ttggcgatga ggaatacctt ttccttcatc
    20761 acggatatga atataaatcc agggattttt atgggtagcg gtaataatga tatttccgcc
    20821 tttttggctc atagcgtcca cgcaattttt aatcagattt tccaatgacc atttaatgag
    20881 gtctttatcc ataagtacca taactccttc aattttgctg atgtaatgaa tattgatctt
    20941 atgtcccagg tgaggcattc tttcccgaaa gtattctact gtttcagaaa taattcggtc
    21001 cagattttgc ggttccagtt tagtgatact gcctactttt ccaaagcggg aagcgattgt
    21061 tttcaaatgt tccaggtcgg cagtcatata ttgcacaatt tgatctatat cgcgcacaca
    21121 gccatctgga gaaggttctt tcaggtaatc cagccatccc attaacgaag ttatgggggt
    21181 tccaaactgg tgagcagttt ctttagctaa actgacccag agggtatctt tttcggttct
    21241 acccagtaaa aacaagccat aaatgccaaa gaaaacaacc atcaaagcca aaatcaattc
    21301 caaaataacg atgtagcgta tataggaaag ggatttggga gtggaaaaat aaatatagcc
    21361 caaactatct gcagcattag aaagcggaat ttcatccatc gtttgaatct gatgagccag
    21421 cttttgctgt tcttccgtag aaatttcata gtaattggct gtttcaggaa tcccgacatt
    21481 tctccagaag aggggttgtt tatagtggtc ggttacaata attgagcaat ctacattttt
    21541 aatgaactcg gcaaaagaaa cgggtttact gtaataggca taaccgctaa ttttgccatc
    21601 atccgttaaa ggcatttgaa ccatcgtctt catcaagttt tccaattttt tctgattttc
    21661 aggggaaagc tcgtgaaaaa tggtatctgt aggcacacca acatttttcc acagcagggg
    21721 ttgaaaattt tgatcggtaa tgattacggg aaccggattt tcctgcataa attcagtgct
    21781 gatataatcc cagaggtctt gtttaaagtc cctggaagta gtaaattgca aatatttgga
    21841 acttatttcg gtaattaatt gggcattgcg ttccgcttct cttaaatatc tatcggtgta
    21901 agcaatatac tgtgcaaaaa tgcgcggaat aaattcctgt tccttttttg cctgtttcag
    21961 gagaaactga atatagacag caaagaaaat aaagattgcc aagctgccga aaatcaggat
    22021 aaaccgaaga cgattgaaga gattagtgct ttttggttct ttcccagtga gaatcgagtt
    22081 cttcaagacc ggcttcatta atgtcttctc cgtttttaag gtaatgttct tcaatagcat
    22141 taaacctgcg agtaaattta cggatgcatt ccttcaaagc ggattctgca tctatatgta
    22201 actttcttgc caggtttacc aaagtaaaaa tcatatctcc cagttcttct ttaatggcat
    22261 cttgttcatt actattcagc gcttcggcaa gttcttctct ttcttcatcc agtttttcca
    22321 gaatgggttt tatatcctgc cagtcaaaac caacggaagc agctttttcc tgagttcttt
    22381 gtgcctgaat aagcgcaggc agtgatttcg gaattccttc caaaacactt ttccgttccg
    22441 ttttttccgc tttttttaaa cgctcccaat tttgtttaac ttcatcggca tcggttaaat
    22501 ggaggtcacc aaaaatatga ggatgacggc ggataagttt actgctaatt tcttccagga
    22561 catcgtctat attccataaa ccctgttctt tagaaatctg tgcctgcata acaatgtgca
    22621 gcatcaggtc gcctaattct tcttttaaag catcataatc cttattttca attgcttcca
    22681 ctacttcata cagttcttca ataaaattgg gaactaagga ctcgcgggtc tgtttcaaat
    22741 cccaggggca acctttggcg ggagttctta aggtagctac tatttctacc agttgttcaa
    22801 atttgttcat attgcctcgg catccagtta tcgggatgcg aaatttcgtt aataatagca
    22861 aaaagtatcc agtaatgatg ttcccaaata atagaactgg gtagaataga agttggcagg
    22921 aacatcaaaa gcgcaaagaa atacatttcg taataatgtc tgttttccgg gttcattttt
    22981 atagcacgcc acaatctata aagccacagc atataaagat aagcaaaacc cagaaagaaa
    23041 actatgccgt aattggtaag cagttccaga aagaaattat gggaattgga aattccgcct
    23101 gttttataca ttcttcctgc gagcatataa tgttccacat ttccggctcc aacaccctta
    23161 aaaccgcttt cagccaatat ttccagattt tggggaatta agcgggtgcg tattttaatg
    23221 ctgctcattg tgaaggactt tgattcctgt ccgatactgc ttatttcttc ggtaaacatt
    23281 tctgtgccaa aggaaacgag gggtttggca aaaattacca aaccgacaat tattaaagcc
    23341 atcaggaata aacttaatct tctcattcgt gcagaataaa gaaaaataaa ggcaaacaga
    23401 aacaaacagg aacaggcaat aatagcaatt ctggcacctt caatgactat aatgcccatt
    23461 atagtgaaaa aacaaagtag ggaaattact ccccaaaact tgctcttttt tatttccgga
    23521 agaaacagga taaaaggaag taacagatta aaagcggcag ctaactgatt aggtccgtaa
    23581 aaaggaccgg taggaacaaa attggtctta ttataataaa gcgattgagg cagatgttgc
    23641 caggtaagca tttcccatag agcaattacg atataaacca aaatcacgga aaaccagaac
    23701 caaggagcat attttttaac cgtctgatga cggcaaaaga tgcttactat aataaaaagc
    23761 attgcttttt tcaatatcag tatggaatga acaaccgaga cctgtttcac gaaagaccaa
    23821 ttataactta aaaaggaata acttgtccaa gccagcataa acattgcagg aaggaaattt
    23881 atcttgttga taacgattaa atccggattt atcaaagcgt agattacaca caggcaggca
    23941 acaaacaaaa aatacatatt gttaattccc attgctaccg gaaagcattc cagaacgaaa
    24001 cttattagca gaaagggagt tatcaggttg cctaatctga tattattgta aggaacgctt
    24061 aaagttgcgg aattcaggag gccttctttc ttccgaaaag agccgcttta attgcctgtg
    24121 ttttttcttt attttctccc tgcaagcttt caattatcaa agcaagaagg caagatagaa
    24181 taaaagcggc tacagtactt ataactactg ttaaagctct tttgggttta gacctgatgc
    24241 ctgccagttg aggtgcatca taaacctcaa aagtaggtag gtctttcacc tcttccagtt
    24301 tggcaagctc aaattgaggg taaaggtatt caattatttt cttttctatt tccaggttca
    24361 gcatcatttg ggcatattgc attgccaaat caggtatttt atctatttgg acgatatatt
    24421 ccggcacgag gtcacttccg cttttttcca aactttttat ttttttcttc aataactgca
    24481 gtttttcagc cagattaata actaccgggg aatcagcaga gtattgggat aacgcaagag
    24541 aatgttcaat ttccgtttgc atatattctg ccacaatatc actatagagt tccagttggg
    24601 cttccgtttg ttgagtaata tcaatagagc ggtttttctt ttggaaatct cttatttgtt
    24661 cggatagaga ataaactgct tgcagatgac tatccacctg tttttccaaa aattcccgtt
    24721 tttggcgtcc tttgctcatt ttactgtgca gaatgtattt attcaaagaa tccaaatgat
    24781 attgaacgat ttgacgggag agttctttat ttttggtttc ggctatgata tttaccaggt
    24841 atgttttctg gtcgtaaaat atctgcattg tggattcgct taattttttt actgccagtt
    24901 ccatagcttt gagggtatca ggttcgttta ttttataata gggtatcaga ttaaactttc
    24961 taatcacctc ttcctgaaat ttacggcttt gcattatgta aatgaaatcc actgccatat
    25021 catatttatc ggtttggaga agaccactgc ctaaaaggtc cataacgcct cctcctaaag
    25081 aacccaaact tcccgattcc gcaataggca tcaaggttgc tttggattcc caatattgag
    25141 gaactaccag tgaatagatg atagcgccaa tagctacaag gaggcaaata cttataacca
    25201 aagttttgcg tttggcaatt accagaataa tatccagcag tcctaattca tttttattca
    25261 ttttccatcc tcttatgagg tcttaaaatt ttttttctct cctgcccgga aaggttcata
    25321 attctcttaa aaactaaatt gttataccga gaaagtggtc caaaacagct ggcagtttaa
    25381 tagttccatc ttcttgttga tatgtttccc aaatggcaat cagcaaacgc ggagtagcaa
    25441 ctccggaacc gtttaaagta tgtaggtgtt tcacttttcc ggttttatcc ttataacgga
    25501 tattagcgcg gcgtgcttga aattcaccaa aagtgctaac tgaactcact tccaggtatt
    25561 ttcctgttcc aggagcccaa acctctaaat cgtaggtttt ttgagaggcg aaagataaat
    25621 cactggtaca aagggctaca actctataat gcaaattgaa tgcctgtaaa atatcttcgg
    25681 catcggagag aatttgttcc agtgcttcag cagatttttc cggttctgta aaatgaacta
    25741 attccacttt attgaattga tgtaggcgtt gcaaaccttt tgtttctttt ccgtaggaac
    25801 cggcttctct tctaaagcaa ggagtgtatg ctacaaattt ttggggcagg tctttgtagg
    25861 aaagcacttc gtcggcaaaa atattggtaa ccggaacttc tgcagtggga atcagaaaca
    25921 aatcatcctt ttcaatccta tacatatctt cttcaagttt gggaagttgt cctgttcccg
    25981 tcatcgtttt ccggtttaca attacgggag ccatcagttc agtgtaatga tgtttctgca
    26041 gatgaaattc cagcataaag tttataagag cgcgttctat gcgagcacct tcgcctgtat
    26101 aaagaggaaa accgctaccg ctgatttttg ctccccgggg taaatccagc agtttatttt
    26161 ggatagccag ttccagatgg tctttgggag taaaggaaaa ttgtttcggt tcaccccaaa
    26221 tttttacgat tttattggct gattcatctt ttccaacggg aacatcctct tgtggaatat
    26281 tgggaatggt tagcagcaat ttttccagtt cgccgtttac aatgctcaat tgagcggaga
    26341 gtgcttttat ttcttccgct gttttggtca ttgcctttag ttcattttca atgttttccc
    26401 cggtcttttt cttttgggca atgagggcag aaacgctgtt ttgatgtgcc tttaagttat
    26461 caaatgcaaa ttgcagttgg cgtctttgtt catcaatttc cagcagttta tctaaatccg
    26521 ccttttcatt tttattgctg attgcttgtc taacgagttc tatattatta cgaataaact
    26581 taatatctaa cattcttgtc ctttactttt cattccaatt tgcaccattt ccagaaaatc
    26641 ctcttctttt aaggaagctc ctccaattag tcctccatca atatcttcgc aatccaaaag
    26701 ttctttcagg ttacctggtt tgatgcttcc gccataaaga agggagagtt tttcggcaac
    26761 cttatcgcta taattgcttt tcagccaatt acgaattaaa ttatgcactt cctgtgcctg
    26821 gaaaggagtg gcagtttttc cagttccaat tgcccaaacg ggttcataag ccaaaattat
    26881 ttcctcacca ctaaccagtt ttatattttt cagacaaccc gaaagctggc ttaaaataac
    26941 attttctgtt ttcccctctt cccgttgttg caatgtttct ccgatacaaa caagaggagt
    27001 tatatgattt tcaaaaagct ttagtatttt agcatttacg gttgcatctg tttcattatg
    27061 gtattgacgg cgttcagaat ggcctacaat acaaaatttc aagcccaaag atgccagcat
    27121 tcctgctgag acctctccgg tgtaagcgcc ttcagaatga acggaaacat cctgagcaga
    27181 aacctgcata caggaaccat aagagccctt cagtgcttct tccagaaagg gataagccgg
    27241 tgcaattaaa ggtattacat tatccaattt aagcttatta agttcaggaa ttactgtctc
    27301 caaaaaattc tttgtttcct gtaaaccttt gttcatcttc cagttaccgg caatatatat
    27361 cttccgcatt agcatacctc acttctttta atccttttta tcactcaaat tttactttgc
    27421 atattaggtc aagtattttt cttttagc
//
LOCUS       DAOOZV010000023        26299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2018_length_26299_cov_64.243027, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000023 DAOOZV010000000
VERSION     DAOOZV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 26299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26299
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2018_length_26299_cov_64.243027"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1945)
                     /locus_tag="PLW08_03770"
     CDS             complement(176..1945)
                     /locus_tag="PLW08_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPL51753.1"
                     /translation="MNEVLLTLEDVSCLYGERIILQGVSFGIHSSEKIGIVGINGSGK
                     TTLLRIISGLIKPNTGTVTMRKDLKVCLLEQDPVYISELSVLQHTFPVAGEIKDEYHY
                     KSILSRLGINNFDEKMGNLSGGQRRKADLARVLAAEPDLLLLDEPTNHLDLETIEWLQ
                     NYLANAGKAVIFVTHDRYFLDAVATKIIEIERTKLFYYEGNYSAYIRGKLIRATDNKR
                     KETRRQAQLKKELDWLNRGAKARTSKPKDHIDRVKELLSKSYLISHQDLDISFQIDRL
                     GKTILELHRLSKSYSGKVLFTDIDHNFQNMERIGIIGPNGCGKTTLLKIILGEEKPDN
                     GTVKIGVNTHFAYYKQDEDSFDPNLTVYEYIAQFAEVIKTAEGNKVTATEMLKRFLFD
                     GKMQQMKLGALSGGERKRLYLLKALMFGANFIIMDEPTNDLDIRTLEILEDYLDAFKG
                     CLLIVSHDRFFLDRTVDYLFIFQDGKLRKFAGNYSDYLLVKRFEQDATEEKKEPVITR
                     PKRIAKGLSYNEQREFGLLEKEIEKIESELMELESRLNDFANPLSPNDYYHISISMQE
                     LENRHQELLTRWLELSDKQNAGN"
     gene            complement(2099..2175)
                     /locus_tag="PLW08_03775"
     tRNA            complement(2099..2175)
                     /locus_tag="PLW08_03775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2139..2141),aa:Arg,seq:ccg)
     gene            complement(2343..3077)
                     /locus_tag="PLW08_03780"
     CDS             complement(2343..3077)
                     /locus_tag="PLW08_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL51754.1"
                     /translation="MKKSFFIVFLLSIMLGSCKFITPPERNTPIICLTFDDQRDGVFT
                     YAFPLMQEYGYRGTCYVNSQYLYGPKNMSLAQIQTLHNDYNWEIGGHSLRHEDLAELT
                     YEEADYTISQDYWNLYNWGFHPRSFAMPRGKCPLEYYPIITKYYEYLRGCNDYAMHIP
                     LNVQGLGYLAFQSSWTADIYKERIRRGIANGEDLIILGFHAIEEPDNIYGTNCPVSEF
                     AEILRYINHLGLEVLPLSEAVDKLNK"
     gene            complement(3074..4093)
                     /locus_tag="PLW08_03785"
     CDS             complement(3074..4093)
                     /locus_tag="PLW08_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51755.1"
                     /translation="MTYKKIKFLLFCLLFGNCAIMNGLEVPLFLRWKSINTGAYCPNQ
                     TGKEIHWNEFYQIGMGIDSLAYKDLFATMELRSRANFIENHIEIYKFDLSWAKNNWEI
                     TAGSIPWGYGLPNKLYSFAPVSADEDQYSYQATRLNRVYLGYNFAENKIAIDVGGDNH
                     NQVTGKLTYQRQKLFGYFQASEEIRAQDNHWQTPVSISSVNTQIQKGNLVLEAQLAFS
                     FLPNYDVTEKHTTTYALLAGKYKINDLTELFGSGEYLELEPTLMSYQQYRIALSRKIS
                     VYSLNPFYYLDYYDKSTFHRIGLSWDWHFCQTQRIGLIYYMEGSKLNKAKHNLGIQAN
                     LELGL"
     gene            4417..5616
                     /gene="coaBC"
                     /locus_tag="PLW08_03790"
     CDS             4417..5616
                     /gene="coaBC"
                     /locus_tag="PLW08_03790"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPL51756.1"
                     /translation="MSGKKILLCVSGGISAYKTIDLASQLSKEGWEVKCVLTDNALKF
                     VTPINFSAITHNSVHTSLFSDIDPIPHITLSAWADLIVVAPATANIIAKAANGIADDL
                     LSTILVACNKPMLFVPAMNVNMYQSVIVQENLRKLKERGNYILQPATGMLACGIEGEG
                     KYPPNTEVVYAIKTYLNYKTDLQGIKVLLTAGATIEAIDPMRYISNRSSGKMGLAIAR
                     ALALRGAEVTLIYGNIQLDIPYYLKEAVFCESSAKMYEAVITRYAEMDWLICCAAVAD
                     YKPKETILQKLPKQEELNLELVPTIDILAELGKLKKANQRLIGFAAQSDNLIQSAIDK
                     YYRKNLDLICANDIRYAGRDDDELIVQGKLKGYPPLKEDPNINSVVMKGDKFLLAHNL
                     INILKEL"
     gene            5613..6353
                     /locus_tag="PLW08_03795"
     CDS             5613..6353
                     /locus_tag="PLW08_03795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL51757.1"
                     /translation="MRQILISGANSQIGNYLAKEYYKEGYKLVLLYHKRKERISELLN
                     KDGIFSCPVDLCNSEAVKEAVFRAEQFLHQSSDTLIHCAAIRSSDALPLYQTEPMQFA
                     NVLSTNMMSAYNILRWTLPSMQENAFGRVIMFGSDVSQKGLQNGSAYSASKAGIVNLV
                     KSVALEMAKYNIVINAISPAPVETNLEEDYSGEYLNFRKRYFAEYLAQVPTGKLVTKE
                     EIKKVTDLLIDENIKNLNGEEIIIDGGV"
     gene            6353..7120
                     /locus_tag="PLW08_03800"
     CDS             6353..7120
                     /locus_tag="PLW08_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPL51758.1"
                     /translation="MKVKFYTIFFFLILMVNLTAMEVPFGEGEKLTFTIKYGLVSAGE
                     ATLEAKSSVYQGSPVWHLSTKAKTYPFFDKIYKVRDTVESWWDKNNLQPYKFSKYLQE
                     GHYRQHRVHIYDHSSLNSTYKKWSFQNQVFENSEMEIPPGTQDLLSAFYLVRTKNLQV
                     GKPIMVNITADGRNMPTEIVVHRKEQQKTIFGNIECLVIEPKLKGEAIFKQSGRILIW
                     VTNDAYKIPVRLESKITIGSFVATLKNAEQVPYKIQI"
     gene            7117..8157
                     /gene="queA"
                     /locus_tag="PLW08_03805"
     CDS             7117..8157
                     /gene="queA"
                     /locus_tag="PLW08_03805"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPL51759.1"
                     /translation="MMPDLLATDTYDYYLPKELIAQFPAEKRTDSRLMQVKRQSREIY
                     HHRFTAILELLKEGDLVIINSSKVFPARLFSTKETGAKIEILLLHQLNENTWQCIAKP
                     GKKLKSPQWLHFSDNLSGYISQGNSEGIREIQFTCKTDFWDEIKRIGHIPLPPYIERS
                     DTILDSERYQTVYAEENGSVAAPTAGFHFDAEILNALKKKGVLFSEVILHIGIGTFRP
                     VKTKRITDHKMHSELATIPEITAAAINLAKKEGRRVVCVGTTSVRSVESFWQDGEMQS
                     GSKWTDLFIYPGFNFKVTDALLTNFHLPKSTLLMMISAFGGLETIKKAYNIAVQEKYR
                     FFSYGDAMFIED"
     gene            8158..8871
                     /locus_tag="PLW08_03810"
     CDS             8158..8871
                     /locus_tag="PLW08_03810"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPL51760.1"
                     /translation="MRNYFYYDILDNTMREYNFLKELCEEPLCVRAGAQNEGLGRANH
                     IWLSPSGGLWFTFDYENNRSLPSFALYLGFCIHQCLQSLFEPLQGKLQIKWTNDIIFS
                     NRKLGGILCNYQPAKRTYIAGIGLNTNNEIDAELGKFGAISLKDILGFEVSNDELCRL
                     IIKSVEDNCRFMENKKTYIDYCNAHLFGKGRFALLEMGGINIEAEIIEIDSNGALSIR
                     NEKGELIAVHTGSILQFLD"
     gene            9004..10662
                     /locus_tag="PLW08_03815"
     CDS             9004..10662
                     /locus_tag="PLW08_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51761.1"
                     /translation="MNKWILIALTAVLILFGCTANRAVPKNEETEPYTGIVTKVAILP
                     LKTIDSSSRNIQKILTVRDFDYVFTKFPQYSLLNMDEVAKQFKMSGYKDVEDLEIEEM
                     KELSEMIGCDILVMGSINSIRSDEFALSMRFFSAKTEELTPLDFKVTNAREQRWKTLE
                     TTFIAKLDDVVSNEVNKIYNIALNNYANGNYAEAEKNLVFALGLDPDLKDAYYYLGSV
                     YYKQGKLEQAIQNLELNLEKNPQHTQTLSILIDIYEKTNQTAKRLNAMEKLATINENE
                     ELWLTIANLYSEQNNIAKAEEALQKALELNPNYIEAKTRLAFLLYDNNRFDEAIPYLE
                     AIFDKYPENELVSTRLANAYQKANRLDDAIAKYENTIKNNPQNTMAYLSAVNLYRLKA
                     TQTSDPAEVAAINKKAIDILNTLITNQPNNAIAYMNMAAIYLSQNKLQDAELYANKAL
                     QKDPTLYLPYIYLATISQSKGTNDYNNFIDLEKQAAKAVGKKASTLKTQRDNARNSAV
                     NNFRKALEYLNNAKARTTDEHAITDINNRIARVNQLINQATATY"
     gene            complement(10756..11202)
                     /locus_tag="PLW08_03820"
     CDS             complement(10756..11202)
                     /locus_tag="PLW08_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51762.1"
                     /translation="MKVKFQYGLAGYTGKADGLVFCYNRRMGRVYARKNISPKLVENH
                     HKMGSITANLHSLLPSTGFKDDLRLYVMRYNGLPENAEKEMYSWVNIYLKMMHQLAKS
                     YPEIDLKTITRDYIYDNDLPCISVKRAVEAGLLPPVTDWELLERTL"
     gene            complement(11807..12466)
                     /locus_tag="PLW08_03825"
     CDS             complement(11807..12466)
                     /locus_tag="PLW08_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL51763.1"
                     /translation="MIYFIYLILKLFLPRKVSKHILQTIARFWAKMVVLSTGSKVVVT
                     GKENLPSSGNICFVSNHQGLFDIPVILGFLGVHTGFVAKRELFRIPVLSQWMREIPCA
                     FIDRRNPRKVIQTFQKSAELIKKGNPMVIFPEGTRSRSDKIGNFHLGSLKLPIMGEAT
                     IVPLAIKGSWRIYEIDKRIHPATVYLKILPPIKPTDDIYKDKQKLSTHLHSQISQALE
                     E"
     gene            complement(12643..14088)
                     /locus_tag="PLW08_03830"
     CDS             complement(12643..14088)
                     /locus_tag="PLW08_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL51764.1"
                     /translation="MERYPNINILLNLPEKYLPKAEFVLRTYCYILRLNPTFLYGKHY
                     EGTHLYYGLPNKFDYPLKIYFQPETAEFFEKRELYPLEKVDFCKFRNEPIPFLFSCSG
                     AIFSFTEETCCFRKDIIASGFYFLTCWHEYILAYYGQTQGRIDYKQSLQYRWDFTETP
                     VVDVYCQMLLYAMEIYCPQFIREISWAEKKRFAVSLSHDIDYWDYWSSSAKLDVFKYN
                     LRTFIKRPFNSLYKITGHLLHKNLIYNPWKTMRSMTRRENEKDVRSTWFLLARKDFPD
                     KRQNYINDVKARVQIMDLLGQQDVGLHGSPQSAFDPLVLAEELANLRDLGFNPTGFRT
                     HYLHFNYQQSFSILEEAGIKYDSTLGYWEHIGFRAGISFPFYPFNIAENRPFRVLEIP
                     LIIMDTTLFSPKAMNLSYISAKRALKRLIDTAEQYQSHLSLLWHNTTFDPIDYPMWGK
                     LYWHIIDYALKKQGWVTSLKDIYEEWVTLSY"
     gene            complement(14091..16451)
                     /gene="lon"
                     /locus_tag="PLW08_03835"
     CDS             complement(14091..16451)
                     /gene="lon"
                     /locus_tag="PLW08_03835"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPL51765.1"
                     /translation="MNYTTKIPRTLPVLHMSNVVMFPYLLMPLVVSDEESKLVIDYAL
                     ANDKLMAFFLDQEKDDTGITELANFGTAVTILRMLRNQDGSISMLLQGSTRIKLQKIV
                     QKNPFIMVDVEAIPEQFEEDTEIQAYRTVALELLEKIAQESNILNREMITGLSNIKQA
                     GRVADIIAGNIDLPISDRQKILETIDLKQRFRYLNNCLAELIKQMKVENHIRSNIQLE
                     MSEDQRRYYLREQMDAIRRELGETDEVSKEIMKWQVLIEQKNLPDYVKETALEELERL
                     ATMQPVSSEYSVVRNYLDWIVNLPWREYSKDRLDLKKIERILEKDHYGLKEAKERILE
                     FIAVKKLKGNLKGPILCFVGPPGTGKTSIGKSVARALSRKFIRMSLGGIHDEAEIRGH
                     RRTYIGAMPGKIIMEIKRQGTANPVFMLDEIDKVGRDFRGDPSSALLEVLDPEQNNSF
                     VDNYINLPFDLSEVLFITTANTLDTIPPALRDRMEIIEFTSYLENDKIEIAKHFLIPR
                     EKENNGLAKEKITFTKAALQEIIRYYVREAGVRNLQRRIGSIFRKIAKEVAMGTHQKW
                     IIKAEDIAKYLGPRKLTLEMANRKPEMGVATGLAWTGYGGEILFCETLRMPGKGNIIL
                     TGLLGEVMKESARIAVSYLKANHSVFIIPPKLFETSDIHIHFPSGAVPKDGPSAGLTL
                     TVALASLFTGQKVRHDIAMTGEITLEGKVLAIGGLKEKLLAAKRAGIKHVVIPEENRE
                     TLSDFPADILAGMEITYVQEIQEAIRILLIPNTETVEQKPKQRRIA"
     gene            complement(16566..17066)
                     /locus_tag="PLW08_03840"
     CDS             complement(16566..17066)
                     /locus_tag="PLW08_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPL51766.1"
                     /translation="MIELETLAKIPLFYDVNSEALAYLQPRFKQLLVNKGTVIIKENT
                     EGDQIYILVKGIVQVTKDLVKGFDEDQLLTEKVLAKLSADSLPTFGENGVLGNGTRMA
                     NIIALSDCELYTLSKADFDNFAEYNYQAGYIVMKNIARKLSESLKDTDDNLVKLATAL
                     YIAVQG"
     gene            complement(17078..18250)
                     /locus_tag="PLW08_03845"
     CDS             complement(17078..18250)
                     /locus_tag="PLW08_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPL51767.1"
                     /translation="MKLFLHPFRKISDRLSIRGEDQKLFIRFLWVCSFIAILLLISAL
                     LAWAFEQKGTQENTIKSFWDGIWWAIVSIATVGYGDKYPVTFQGRLVGIILIIVGYSS
                     LSFFTGLIASLFVEDRLKGAKGLKTIRTHNHIVICGWNNTAEFFLKALLEKQVSEMDI
                     CIVINATPDFFERLESRFPTLSLKFVRGDAIQEETLKRASVDTSAQVIILADEQLDPS
                     VADDHSIIVANAVHYLTKNDKITVQLVNPENRSMLQRLGIRNIIIWDDIGGYILANSI
                     ADNNYLAVFCQLVQDKQNHLQTQKIPSDFIGKSFGDLSDYYYRECGYLLLGLLTKEPG
                     LELSSIFTEDISGIDQFIQYALTKSQKKIREEKSNIRWKPEKDVQLQKNDYAILMI"
     gene            complement(18254..18832)
                     /locus_tag="PLW08_03850"
     CDS             complement(18254..18832)
                     /locus_tag="PLW08_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51768.1"
                     /translation="MIFLKSLTFILWNIALGTLLVLLLNWLLFNRKARYLFGKKIPLT
                     PGFFVAKRDWLFDKVRSILHDYLDQAAHPYLKDGYLYGWIKKVRQYLWEKTSFIDEWR
                     FLPAKLKLLVRNKIVDAFTAIAESILRKTVPRLVEQLQIEHRIDEFDIQFSVDFFYGY
                     FKRYVYKPMLLICAGLNLLIGILNMVWFLIIV"
     gene            complement(18833..19594)
                     /locus_tag="PLW08_03855"
     CDS             complement(18833..19594)
                     /locus_tag="PLW08_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPL51769.1"
                     /translation="MKYKRFCAVLAFVFLTALLFSVVVPKPVGFVNDFANILSAETRN
                     KINDWAIELREKTDVDFVIVTLPDIGGEDIVDFGVKLYEQWGIGSKRDEGILVLLALK
                     ERKLRFEVGYGSEGYLTDAYISEVYQTMKSYLTKGNEKWDDAFIQGSLMLLSTIAKEK
                     GITLTGVSEYSQKKKESSGGVGLLAFLILFIILIIATRGRILEWLFWMSIFGGRGGGG
                     NSGSWSSGSGGFGGFGGFGGFGGGRSGGGGGGGGF"
     gene            complement(19584..20816)
                     /locus_tag="PLW08_03860"
     CDS             complement(19584..20816)
                     /locus_tag="PLW08_03860"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPL51770.1"
                     /translation="MTNPEQEVDKTIFASIEEAIEIIRKGDMLIVVDDENRENEGDLL
                     MAADKITPEKVNFMISQGKGLLCAPLDIEIANRLDIPLMTNKSSDRQGTKFAVSVDVI
                     EGTTTGISAIERAKTIKTLADPQANASMFMRPGHIFPLIAERGGVLRRAGHTEAAVDL
                     AKLAGLSPVGAICEIIREDGEMARLDDLLPFAKKHNLKIITIEALIHWRRKKERLVHP
                     VSKAQLPTKYGQFDIITYISDISDEYHIALVMGEIKRDEPILVRVHSECLTGDALFSL
                     RCDCGEQLARAFQMISEEGKGVILYMRQEGRGIGLLNKIKAYHLQDEGVDTVQANIKL
                     GFPPDLRDYGIGAQILKDLGIRKMRLMTNNPKKIVGLSGYGLEIVERVPIEIKPQENN
                     REYLKTKKNKMGHILNEI"
     gene            complement(20809..21843)
                     /locus_tag="PLW08_03865"
     CDS             complement(20809..21843)
                     /locus_tag="PLW08_03865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPL51771.1"
                     /translation="MKDKLKVIYTLQEMQRLDKFLAELKIQELYSRTFIEHLIEEERV
                     LVNLTPVKKSYLLKEGDEVIINLPEPEPMNVLPQDIPLDVVYEDEHLAIINKKAGMIV
                     HPGFGNPEGTLVNAILYRFGENLSSGKEFNRPGIVHRLDRGTSGLIIIAKNDLTQAKL
                     SDMFARREIKKTYFAITTGIPEPEKGEIETYLARSNKNPRTICVTDEGRYALTHYKTI
                     KTWHYFALVEINPATGRMHQIRVHFAHIRYPILGDLLYNSRRYVQSLVPQNMKKKVTE
                     LLLNHLRRQALHSWKLEFLHPITGYPLSVSAPIPDDIQYTLNWLDNNFSIDTDTSDAN
                     LILNEEIYND"
     gene            complement(21833..22570)
                     /locus_tag="PLW08_03870"
     CDS             complement(21833..22570)
                     /locus_tag="PLW08_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51772.1"
                     /translation="MKRSFKARYHILIAILFGIIVLQTLALLFSVARAQDLTTLANDI
                     HSIVIIFVFIIFVYIVILYNYIPFRLHKAIREVEQLIEEISNGNYQIDIDSSIYDQDK
                     DFQELIYALDKMLGIIIRFDQAKADKIFEHHQRLQLLINLLPQSILITGANGDVVYCN
                     EPLRKRFPMISETVNLNELIWKNDYHNRIFSVILDSLRYGNNIYDIIVEDVVYLGKVK
                     INGSIVRNRKGLSTGAVYVLEFYDNER"
     gene            complement(22577..23281)
                     /locus_tag="PLW08_03875"
     CDS             complement(22577..23281)
                     /locus_tag="PLW08_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPL51773.1"
                     /translation="MARYAVIGLGRFGMTVAKILTENGMEVIAIDKDQDLVNEISSIV
                     TSAVCMDSTDEAALRSQNLNEADAVIIGIGSNIEECILTAAILKKIGVGIIYAKIESE
                     LHGRILKMLGVQHTLLPEEIVGNQLAKTLISRNVLDYINLSSGHIVVEMVAPKEFVGK
                     PLQELALPTKRGVNVIAIKYNYLSVTEDGQNVIEKRLNDMPGANDIINEGDVLILLGP
                     KGNIDKLIYETAVKKD"
     gene            complement(23311..25005)
                     /locus_tag="PLW08_03880"
     CDS             complement(23311..25005)
                     /locus_tag="PLW08_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPL51774.1"
                     /translation="MPAEKKSGTFKHIELIAYILAFWSFFVLFLEPIFEYYTSSYPTI
                     EFITALANLVLLVLTILSRVLSREVKENKTVVYFDLVMLILGSLLLTYQAKFVIFFLL
                     IRQTWFIVQFILFRAFEGKIYKLLMRNPPVSLMFSFAGVILIGTILLMLPASSTEGYI
                     TNFVDALFTATSATCVTGLIVVDTGTYYSLFGQIVILLLIQVGGLGIMTISTAFALML
                     GQRISLNVTNLMHNVVGKTPVIDVLKLLRSTVLVTLIIEIIGALFLFTSFAKLMPAGQ
                     AIYYSLFHSISAFCNAGFSLWSNNLAGFVGSPWVIFSFSFLIILGGLGFAVLIDIYHY
                     FFSSRRVRKLALHSKIVLSTTAILIVTGFLGLYIGEYNNSMKGFSVAGRVLSSWFQSV
                     TARTAGFNTIKIGSYSSASVLLTVLLMFIGASPGSTGGGIKTTTFSTLMLSVLSMLTG
                     KRDLVVFKRKISLANAREATTLATLALAIILTIIFILMLIEPFPFEKILFEAFSAFGT
                     VGLSLGITSQLSYAGKLLITLLMYIGRIGPLTLVYAISIRKRQPHISYAEERISIG"
     gene            complement(25020..25652)
                     /locus_tag="PLW08_03885"
     CDS             complement(25020..25652)
                     /locus_tag="PLW08_03885"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPL51775.1"
                     /translation="MLDVIVFLGIQGSGKGTQAELLAERTGFQHINIGDLFREEVHKQ
                     TELGKKVQAIIAKGELVQDELVFEIVNASLTADCAGVVFDGFPRTLAQAEYLLKNYNV
                     RRVYYLELSEEEAIARIEARRLCSSCGETYNILSKKPKQEGICDKCGSPLVQRTDDSG
                     EAIRERVKAFYEQTFALKEYFAEKGLLKEISARKSIEEIQQEIINDLKIR"
BASE COUNT         8362 a         5274 c         4624 g         8039 t
ORIGIN      
        1 ttttagctat gttatcctgg ttgctaacac ttttccccac aagcattact atagcaatac
       61 ggactcattg ctaatgccat ccgtaatggg taagcaatgg gtaagcaatg caattaggga
      121 aggcaacaaa aatcattata aaatataaag ttacgcttcc tgtaaaaaaa tactgtcaat
      181 tccctgcatt ttgcttatcg ctcagttcca gccaacgcgt aagtagttcc tgatgtctgt
      241 tttccaactc ctgcatagaa atgctgatat gataataatc attgggagag agaggattgg
      301 caaaatcatt aagtcggctt tccagttcca taagctcact ttctattttt tcaatttcct
      361 tttccagcaa gccaaattcc cgttgttcat tatagctcaa gcccttggca atgcgtttgg
      421 gacgtgtaat aaccggttct tttttctcct ctgttgcatc ctgttcaaaa cgcttaacca
      481 gcaaataatc ggaatagtta ccagcaaatt tacgtaactt accatcttgg aaaataaaga
      541 gatagtccac cgttctatcc aaaaagaaac gatcgtggga aacaattaac aggcaacctt
      601 taaaggcatc caggtaatcc tccagaattt ccagagtgcg aatatccaag tcatttgtgg
      661 gctcatccat aataataaaa ttggctccaa acatcagtgc cttcaagaga tagagccgtt
      721 ttctttctcc tccggaaagt gcgcctaatt tcatttgttg cattttgcca tcaaaaagaa
      781 atcttttcag catttccgtg gcagtaactt tgttgccttc agcggttttt attacttcgg
      841 caaattgagc gatgtattca taaacggtaa gattggggtc aaaactatcc tcatcctgtt
      901 tataataagc aaaatgggta tttacaccta ttttcacagt tccgttatcg ggcttttctt
      961 ctcccaggat aattttcagc aaagttgttt tgccacagcc attaggaccg ataattccaa
     1021 ttctttccat attctgaaag ttgtggtcta tatccgtaaa aaggactttg ccggagtagg
     1081 atttgctgag acgatgcaat tccagaatgg ttttccctaa ccgatcaatt tgaaaagaga
     1141 tatccaaatc ctgatgggaa ataagataac ttttgctgag cagttccttt acccggtcaa
     1201 tatggtcttt gggcttactg gttcttgctt ttgcaccccg atttagccaa tcaagttctt
     1261 tttttaattg tgcctgacgc ctagtttctt tacgcttatt atcagtagct ctgattagct
     1321 ttccccgaat gtaggcactg taatttcctt cgtaataaaa gagtttcgtc ctttcaattt
     1381 caatgatttt ggtggcaaca gcatccagaa aatagcggtc gtgagtaaca aaaataactg
     1441 ccttcccggc atttgccaaa tagttttgca gccattcaat ggtctccaaa tccagatgat
     1501 tagtgggctc atctaaaagc aataaatccg gttcggcagc taaaactctt gccaaatcgg
     1561 cttttctgcg ttgccctcca gaaaggtttc ccattttttc gtcaaaattg tttattccta
     1621 aacggctgag aattgattta taatgatatt catcctttat ttctcctgct acgggaaaag
     1681 tatgctgcaa aaccgaaagc tcggaaatat aaacagggtc ttgctctaac aggcacactt
     1741 ttaaatcctt acgcattgta acggttcccg tgttgggctt gattaaaccg ctgataatac
     1801 gcagtaaagt tgttttgcca ctgccgttta tacctacaat gccaattttt tcggaagaat
     1861 gaataccaaa agaaacaccc tgtaaaatga ttctctcgcc atatagacag gatacatctt
     1921 caagggttaa taggacttca ttcatatttt tttcccgcag atttcgctaa ttttcacgca
     1981 gattacgcag atttttatct tcatttaata tcaacatata taattagtta tactactgca
     2041 ttctatttat tgcgcgggtt tcactttttc acttcacttg ttaacccgtt aatcattttg
     2101 gcgtccctga ggcgattcga acgcctgacc ttcaccttcg gagggtgcca ctctatccat
     2161 ctgagctaca gggacgcaat tttctaacgc attatttctg caaaggggtt ttatgtcaat
     2221 caagaagtaa aagagaacaa gaaatcttta gtcctaataa agtatgtgta gacggaggac
     2281 acctttttcc cataaagata aaaggataga aaaatgcaaa gattcagcga gcttcgttta
     2341 cactatttat tcagtttatc tactgcttcg gaaagaggta aaacttccaa tcccagatga
     2401 tttatatagc gtaatatttc cgcgaattcc gataccgggc aatttgttcc atagatatta
     2461 tccggctctt caattgcatg aaaaccaaga attatcaggt cttctccgtt agctatacct
     2521 ctgcggatgc gttctttata gatatcagct gtccaggaac tttgaaaagc tagataaccc
     2581 aaaccctgaa cattaagagg aatgtgcatt gcataatcat tacaaccacg taagtattca
     2641 taatatttag ttataatagg ataatattcc aaaggacatt ttcctctcgg catagcaaaa
     2701 gaacgcggat gaaaacccca attataaagg ttccagtaat cctgactgat agtatagtca
     2761 gcttcttcat aagttaattc agccaaatct tcatgacgta gagagtgacc tccaatttcc
     2821 cagttataat cattatgcaa ggtttgaatt tgagctaaac tcatattttt aggtccgtaa
     2881 agatattggg aattcacata acaggttcct ctatagccat attcctgcat caaaggaaaa
     2941 gcataagtaa aaactccgtc ccgttgatcg tcaaaagtaa gacaaattat tggggtattc
     3001 ctttctggcg gagtaataaa cttacaggaa ccaagcataa tagagagtaa aaacacaata
     3061 aaaaacgact ttttcataat cccaattcca gatttgcctg aattcccaaa ttatgtttgg
     3121 ctttattcaa tttactgccc tccatataat atattaatcc gattcgctgt gtttggcaaa
     3181 aatgccagtc ccaggataag ccaattctat gaaaggtgct tttatcataa taatcaagat
     3241 agtaaaaagg atttagggaa taaacactta ttttacggct taaagctatt ctatattgct
     3301 gataactcat taaagtcggt tccaattcca gatattctcc actgccaaat agctcggtta
     3361 agtcatttat cttatatttt cctgccagaa gagcataagt agttgtatgc ttttcagtta
     3421 catcataatt gggaagaaaa ctgaaagcca gttgtgcttc taaaacaagg tttccttttt
     3481 gtatttgtgt atttacagaa ctaatggaaa caggtgtttg ccagtgatta tcttgagctc
     3541 taatttcttc tgaagcttga aaataaccaa agagtttctg tctttgataa gttaattttc
     3601 ctgttacctg attatgatta tcacctccaa catcaattgc tatcttgttt tcggcaaaat
     3661 tgtatcctaa ataaactcta tttaaacgcg ttgcctgata actgtattga tcctcatcag
     3721 cagaaacagg agcaaatgaa tataatttat taggtaatcc ataaccccaa ggaattgaac
     3781 cagctgtaat ttcccagtta tttttagccc aggaaaggtc aaacttatat atttctatgt
     3841 ggttttctat aaaattagct ctactgcgaa gttccattgt agcaaaaaga tctttataag
     3901 caagggaatc aatacccatt cctatctggt aaaattcgtt ccagtgaatt tcctttcctg
     3961 tttggttggg acaataggca cctgtattaa tagatttcca acgcaagaat aaaggaactt
     4021 ccagaccgtt cataatagca caattgccaa acagcaaaca gaaaagcagg aatttaattt
     4081 tcttgtatgt cataatattt cactttaata gtaagtaata tctgttaata attatatgta
     4141 tgttgtcttt cattgtgttg ctcttttttt tagcttattt cttttattaa taaagcccca
     4201 tatctgtcaa atcctttttt gttgtgtagc cgtaggactt cattcctccg ctttttcttt
     4261 ataccgagct tccgtgagct ccgtctacac aaaatccttt tttcagacct tttaatctct
     4321 taacttcttg cgctcttgac ttcttgacct tttttattga catcaaatca ggaattaaaa
     4381 ttaaagtaaa agccaaataa agatatagag gaaacaatgt ccggaaagaa aatattactc
     4441 tgtgtaagcg gaggcatatc tgcttacaaa acaattgact tagcgagtca gctaagcaaa
     4501 gagggttggg aagttaaatg tgttttaacc gataatgccc tgaaatttgt aacacctata
     4561 aattttagtg ccataacgca taacagtgtg catacttctt tgttttcaga tattgaccct
     4621 attcctcata ttacgctttc cgcttgggct gatttaattg ttgtagctcc cgcaacagca
     4681 aatataatag ctaaagcggc aaatggcatt gcggatgatt tactttccac aattttagtt
     4741 gcctgtaata aaccaatgct ttttgttccc gcgatgaatg taaatatgta tcaatccgtt
     4801 attgtgcagg aaaatcttcg gaaattaaaa gagcggggaa attatatttt acaaccagca
     4861 acaggtatgc ttgcctgtgg cattgaagga gaaggaaaat acccacccaa tacggaagtt
     4921 gtttatgcta taaagaccta tttgaattat aaaacggact tgcagggaat aaaggttttg
     4981 ttaactgccg gtgcaacaat tgaagcaatt gaccctatgc gttacattag taatcgttct
     5041 tccggaaaaa tgggtctggc aatagctcgt gctttagctt taaggggggc agaagttact
     5101 ttgatttatg gcaatattca gcttgatatt ccttattatt tgaaagaagc ggttttttgt
     5161 gaaagttccg ctaaaatgta tgaagcagtt ataactcgtt atgcagaaat ggattggctg
     5221 atttgttgtg ctgctgtagc ggattataaa cccaaagaaa caatactcca aaagcttccc
     5281 aaacaggaag aactgaactt ggagcttgtt cctaccatag atattttagc agagctggga
     5341 aaattgaaaa aggcaaatca gaggttaatc ggttttgcag ctcaatccga taatttaatt
     5401 caaagtgcca tagataaata ctatcgtaaa aatctggacc tcatttgtgc caatgatatt
     5461 cgttatgcag gcagggatga tgacgaactt attgtgcagg gaaaacttaa gggctatccg
     5521 cctttgaaag aagaccccaa tatcaattcc gtggtgatga aaggagataa atttctgcta
     5581 gcacataatc tgattaatat tctcaaagaa ctatgaggca gattttaata agtggagcca
     5641 attctcagat tggcaactac ctggctaagg aatattacaa ggagggatat aaacttgtac
     5701 tactctatca taaacgcaag gaacgcattt ctgagttatt aaataaagat ggcatatttt
     5761 cctgtcctgt ggatttatgc aattccgaag ccgtaaaaga ggcagttttt agagcagaac
     5821 aattcctgca tcaaagttcc gataccctaa ttcattgtgc cgcaattcgc agttcagatg
     5881 ctttgcctct ctatcaaaca gagccgatgc aatttgccaa tgtcttatca acaaatatga
     5941 tgagtgctta taatattttg cgttggactt taccttcaat gcaggaaaac gcgtttggaa
     6001 gagtaataat gtttggaagc gatgttagcc aaaaggggct tcaaaatgga agtgcctatt
     6061 ccgcttccaa agcaggtata gtaaatttgg taaaaagcgt tgctttagaa atggcaaaat
     6121 ataatattgt aatcaatgca atttcaccag ctccggtgga aacaaatctg gaagaggatt
     6181 acagcggaga atatttaaac ttccgcaagc gctattttgc agaatattta gcgcaggttc
     6241 ctaccggaaa attagttaca aaggaagaaa taaagaaagt gacagacctg ctgattgatg
     6301 agaacataaa aaatctcaat ggagaagaaa tcattataga cggaggtgta taatgaaggt
     6361 caaattctac actatcttct tcttcctgat tttgatggtg aatttgacgg caatggaagt
     6421 cccttttggg gaaggagaaa aacttacctt cactattaaa tatggattgg taagtgccgg
     6481 agaagcaact ttggaagcta aaagttcagt ttatcagggt tctccggttt ggcatctttc
     6541 tactaaagcc aaaacctatc ccttttttga taagatttat aaagtgcgtg atacggttga
     6601 aagctggtgg gataaaaata atcttcagcc ctacaaattt agtaaatatt tacaggaagg
     6661 gcattaccgt cagcatcggg ttcatattta cgatcattcc tctttaaatt ctacctataa
     6721 aaaatggagt ttccaaaacc aggtatttga aaacagcgaa atggagattc ccccaggaac
     6781 tcaagacctt ttaagcgctt tttacctggt gcgaacaaaa aatctgcaag taggaaaacc
     6841 gataatggta aatataacag ctgacggcag aaatatgcca accgaaattg tagttcatcg
     6901 gaaagaacag caaaaaacca ttttcggcaa tatagaatgc ctggtaattg aacccaaact
     6961 caaaggagaa gcgattttca aacaaagcgg caggattttg atttgggtta ccaacgatgc
     7021 atacaaaatt ccagtgcgtt tggaaagtaa aataacaatt ggcagtttcg tagccaccct
     7081 taaaaatgct gagcaggttc cctataaaat tcaaatatga tgcctgatct tttagcaact
     7141 gatacttacg attattacct gcccaaagaa ttaatagctc aatttcctgc agaaaaaaga
     7201 accgattcgc gtttgatgca agtaaaaaga caaagcagag agatatacca tcaccgtttt
     7261 actgctattc tggaattgct gaaagaaggg gatttagtca tcatcaatag cagtaaggtt
     7321 tttccggcac gccttttttc caccaaagaa accggtgcta aaatagaaat tttactcttg
     7381 catcaactga acgaaaacac ctggcagtgt atagctaaac cgggaaaaaa attgaaatct
     7441 cctcaatggc tacatttttc cgataattta agcggttata tcagccaggg aaatagtgaa
     7501 ggaattagag agattcaatt tacctgtaaa actgatttct gggatgaaat taagcgtatc
     7561 ggtcatattc ctctgccacc ttatattgaa cgcagtgata caattttgga tagtgaaaga
     7621 taccaaaccg tttatgccga agaaaatggc tctgttgctg cgcctactgc tggatttcat
     7681 tttgatgctg aaattctaaa tgccttaaaa aagaaaggcg ttcttttcag cgaagttatt
     7741 ttacatatcg gcattggaac ctttcgtccg gtaaaaacta agcgcataac agaccacaaa
     7801 atgcattccg aacttgctac tatacccgaa ataacggctg ctgctataaa tttagctaaa
     7861 aaagaaggaa gaagagttgt ttgtgttgga acgaccagtg tgcgcagtgt agaaagtttt
     7921 tggcaggatg gagaaatgca aagcggttcc aaatggacag acctttttat ttatcccggt
     7981 tttaatttta aggttacaga tgctctgcta accaatttcc atttgcccaa atctacccta
     8041 ttaatgatga tctctgcctt tggtggcttg gaaacaatta agaaagctta taatattgct
     8101 gttcaagaaa aatatcgttt tttcagctat ggcgatgcaa tgtttattga ggattgaatg
     8161 cgtaattact tttattatga tatattagac aacacaatga gggaatataa ttttttaaaa
     8221 gaattatgtg aagaacccct ttgtgtgaga gctggggctc aaaatgaagg tttaggtagg
     8281 gcaaatcata tctggttatc gccttcaggc ggtttatggt ttacttttga ttatgaaaat
     8341 aacaggtcac ttccctcttt tgctctatat ctgggttttt gtattcatca atgtttacaa
     8401 tccctttttg agccattgca gggcaaattg caaattaagt ggactaacga tattattttc
     8461 tccaatcgta aattaggagg tattctctgt aattatcagc cggctaaaag aacttatatt
     8521 gcaggaatcg gcttaaatac caataacgaa attgatgcgg aactgggtaa attcggagct
     8581 atttctctta aagatatttt gggctttgag gtctccaatg atgaactttg ccgtttaatt
     8641 attaaatccg ttgaagacaa ctgccggttt atggaaaata aaaaaaccta tattgattat
     8701 tgcaatgcac atctttttgg aaaaggacgc tttgccttac tggaaatggg gggaattaac
     8761 attgaagcag aaattattga aattgacagc aatggtgctt tatcaatcag aaatgagaag
     8821 ggagaattga ttgctgtgca tactggttcc attcttcaat ttctggattg aaacatatat
     8881 tttctttgtc acgcacataa aaatcgtttt actataagcc aaaaacggtt ttgcttgaca
     8941 gatacactct ttgtaaaagt tggttaaaca aaattatttc gatcgctata ctctggagga
     9001 aaaatgaata aatggatact gatagcgtta acagctgtgc ttattctctt tggctgcaca
     9061 gcaaatcgcg cagtccctaa aaatgaagag accgaacctt atacgggaat tgttactaaa
     9121 gtagctattt taccccttaa aacaattgat tcttcaagcc gtaacatcca gaagatttta
     9181 actgtccgtg actttgatta tgtttttaca aaatttcccc aatattctct tttgaatatg
     9241 gatgaggtag ctaagcaatt caaaatgtcc ggttataaag atgtggaaga cctggaaatt
     9301 gaagaaatga aagaattatc tgaaatgatt ggctgtgata tcctcgttat gggtagtata
     9361 aatagtattc ggagtgatga atttgctttg tcaatgcgct ttttcagcgc taaaaccgaa
     9421 gagcttaccc cgcttgattt taaggttacc aacgcccggg aacaacgctg gaaaaccttg
     9481 gaaaccactt ttattgccaa actggatgat gttgtttcca acgaagtgaa taaaatttat
     9541 aatatcgctc tcaataatta tgccaatggc aattatgcag aagcggaaaa aaatctcgtc
     9601 ttcgctttag gtcttgatcc cgatctcaaa gatgcctact attatcttgg ttccgtttat
     9661 tataaacaag gaaaattgga acaggcaatt caaaatctgg aattaaatct ggaaaaaaat
     9721 ccccagcata ctcaaaccct ttccattctg attgatattt acgaaaaaac caatcagacc
     9781 gctaaacgct taaatgctat ggaaaaactt gctaccatca atgaaaacga agaattgtgg
     9841 ctaaccatag ctaatttgta ttcggaacaa aataatattg ctaaagcgga agaagcttta
     9901 caaaaagctc tggaactgaa ccctaattat attgaagcaa aaacgcgttt ggcttttctt
     9961 ttgtatgata acaaccgttt tgacgaagca atcccttatc tggaagccat atttgataaa
    10021 tatccggaaa atgaacttgt ttctacgcgt ttagccaatg cctatcagaa agctaatcgt
    10081 ttggatgatg ccattgccaa atatgaaaat acgatcaaaa ataatcctca aaacactatg
    10141 gcatatttaa gtgctgtgaa tctatatcgt ctaaaggcaa ctcaaacatc cgatccagca
    10201 gaagttgctg ccatcaataa aaaagctatt gatatcttaa atactttgat taccaatcaa
    10261 cccaataatg ctattgcata tatgaatatg gcggcaatct atttaagcca aaataaatta
    10321 caggatgccg aattatacgc taataaggcc ttacaaaaag acccaaccct atatttgccc
    10381 tatatttatc ttgctactat cagtcaaagc aagggaacta acgattataa caatttcatt
    10441 gatctggaaa aacaagctgc taaagccgta ggtaaaaaag caagcactct taaaacccaa
    10501 cgcgataacg caagaaattc cgctgtcaat aatttccgta aagccctgga atatctcaat
    10561 aatgctaaag cccgaactac tgacgaacat gctattaccg atatcaataa tcgtattgcc
    10621 agagttaatc agctcataaa tcaagcaaca gctacttatt aatcacaata atacagttga
    10681 tatattagca ggttggcagg ttagcgagta aataggtaaa aattactcgc taaccttttc
    10741 acctattaac cttctttaca aagttctctc cagcagttcc cagtccgtta ctgggggcag
    10801 taaacccgct tccacagccc ttttaacact gatgcaaggt agatcattat cgtatatata
    10861 atcccgggta atagttttta ggtctatttc cggatacgat tttgccagtt gatgcatcat
    10921 cttcaaatag atattcaccc aggaatacat ctccttttcg gcattctctg gtaaaccgtt
    10981 atagcgcatc acatagagcc gaaggtcatc tttaaaaccg gttgagggca gtaacgaatg
    11041 cagatttgcc gttattgaac ccattttatg atgattttcc accaattttg gcgagatatt
    11101 cttccgggca taaaccctgc ccattcgccg attgtaacag aatacaaggc catctgcctt
    11161 gcctgtgtaa cccgctaagc catattgaaa ttttactttc attttcgttc tccttatttt
    11221 gtccttctat aatatatacg ccggaaaaaa atgcgaagtg gtgacaaaaa tgccaaaaaa
    11281 ctttcattcg gacaaaaata atttttaatt gaaggacgaa tcctgctctc tgtagccagt
    11341 tataacaaag taacaatcaa gtaacaattc cgtaacgaga caacggaagt gttacggaag
    11401 tgttacggag tcgttactta attgactatg taacaaacaa ttaatgaagg ttgaaaaatg
    11461 ataaatttga aaattgagta ttcagaattg agaattgaaa atagttgaaa agggtgaggt
    11521 ctttaaaatc ccgatgggcg acacaatgat gtagaaggtg gcgtaagccc ctcggaaaat
    11581 gaggtttaga aggttgagaa ggtttagagg gtttagatac attaaagccc cgaagggcga
    11641 cactttcata gcgatggtgg cgtaagcccc tcggaaaata aggtttagaa ggttgagaag
    11701 gtttagaggg tttagataca ttaaagcccg tcagggcaat tctcaatttt cacttctcaa
    11761 tttgcaattt gcaattctca cctgatatct ttataaaatt catttttcat tcttccagtg
    11821 cttgagaaat ttgtgaatgc aaatgggtgg aaagtttctg tttatctttg tagatatcat
    11881 ccgtaggttt aataggaggc aatattttta aataaacagt agcaggatgg attcttttat
    11941 caatttcata aattcgccag ctacctttta tcgctaaagg gacaattgtt gcttccccca
    12001 taatgggtaa ttttaagctt cccaaatgaa aattgccaat tttgtcgctt ctgctgcgtg
    12061 ttccttccgg aaaaataacc attggatttc cttttttgat tagttctgca cttttttgaa
    12121 aggtctgaat taccttacga ggatttctgc ggtcaataaa agcgcaaggt atttccctca
    12181 tccattgact caataccgga attctgaaaa gttccctctt agccacaaaa ccggtatgaa
    12241 ctcctaaaaa cccaagaatg acaggaatat caaacaaacc ctgatggtta ctgacaaaac
    12301 atatatttcc gctggaaggc agattttcct tacctgttac cacaaccttg ctgccggtag
    12361 aaagcacaac catttttgcc caaaacctgg ctattgtctg caaaatgtgt ttggaaacct
    12421 tacgcggtaa aaaaagtttc aggataagat agataaaata aatcataagg tagacaagaa
    12481 aagaaagaca aaagagaatt tcccaaagga gggatatgat tttcatactg agaattaagt
    12541 tagctgaaat ttatggattc ctgctttcgt aggaaggaca aagctctcag gaaggacaag
    12601 gaactcttca ttaaaaaaat gatatgattt catagcagtt ttttaataac taagggttac
    12661 ccactcttca taaatatctt tcagggaagt tacccaaccc tgtttcttaa gagcatagtc
    12721 aataatatgc caatacaatt taccccacat tggataatca attggatcaa aagtagtatt
    12781 atgccaaagt agagaaagat gtgattgata ttgttccgcg gtatctatta atcttttcaa
    12841 ggcacgttta gcagaaatat agcttaaatt cattgctttt ggggaaaaaa gagttgtatc
    12901 cattataata agaggaattt ccaaaacgcg aaaaggtctg ttttcggcta tattaaacgg
    12961 ataaaaagga aatgaaatac cagcccgaaa acctatatgt tcccaataac ccaaagtgga
    13021 atcatacttt attcccgcct cttccaaaat ggaaaaactc tgctgataat taaaatggag
    13081 ataatgagtc ctgaagccgg tcggattaaa tcccaggtct ctcaaatttg ccaattcttc
    13141 agctaaaacc aaaggatcaa atgctgattg gggactgcca tgtaagccaa catcctgctg
    13201 acccagtaaa tccattatct gaactcttgc tttcacatcg ttgatataat tctgacgctt
    13261 atccggaaaa tctttacgtg ctaataaaaa ccaggtggaa cggacatcct tttcgttttc
    13321 gcggcgggtc atagaacgca ttgtcttcca gggattgtaa ataagatttt tatgcaaaag
    13381 atgacccgta attttataga gtgaattgaa aggtcgtttg ataaaagtcc gcaggttata
    13441 tttaaaaaca tccaatttgg cagaactgct ccaataatcc caataatcaa tatcatgaga
    13501 aagagacacg gcaaaacgct ttttttcagc ccagctgatt tctctgataa actgtggaca
    13561 ataaatttcc atagcataaa gcaacatttg acaatagaca tctacaaccg gtgtttccgt
    13621 aaaatcccaa cggtattgca agctttgttt atagtcaatc cttccctgtg tttgtccata
    13681 ataagccagg atatattcat gccagcaggt taaaaaataa aacccgctgg caattatatc
    13741 cttacgaaag caacaggtct cttcggtaaa agaaaagata gctccgctac aagaaaagag
    13801 aaaaggaatc ggctcgttgc gaaacttgca aaaatccact ttctccagag ggtataattc
    13861 cctcttttca aagaattcag ctgtttccgg ctgaaaataa attttcaacg gataatcaaa
    13921 tttgttcggt aatccgtaat atagatgcgt gccttcatag tgcttaccat aaagaaaggt
    13981 aggattcaaa cgcaaaatat aacaataagt gcggaggaca aactccgctt tcggtaaata
    14041 cttttccggc agattcaaga gaatattaat attgggatag cgttccaaaa ttaggcaatc
    14101 cttctctgtt tcggtttctg ttcaactgtt tccgtattgg gaataagcaa tattctgatt
    14161 gcctcttgaa tttcctgcac ataagttatt tccattcctg ccaaaatgtc tgcaggaaaa
    14221 tcggacaggg tctcgcgatt ttcttccgga atcaccacgt gtttaattcc tgctcttttt
    14281 gccgccaaaa gtttttcttt caagccaccg atggctaaaa cctttccctc taaagtgatt
    14341 tcacctgtca tagctatatc gtggcgcact ttttgtcccg taaaaagtga agctaaagct
    14401 accgtaagag ttaaaccagc tgaaggtcca tccttaggca cagctccaga aggaaaatga
    14461 atatggatat cacttgtttc aaaaagttta gggggaataa tgaaaacgct gtgattagct
    14521 ttcaaataac taactgcaat gcgtgccgat tctttcatca cttcgcccaa aagtcctgtt
    14581 aaaataatat ttccttttcc cggcatacgc aaggtctcac agaaaaggat ttcaccccca
    14641 taacctgtcc aggcgaggcc tgtagccact cccatttcgg gtttgcgatt tgccatttcc
    14701 aaagttaatt tacgcggacc taagtatttg gcaatatcct ccgctttaat tatccatttc
    14761 tgatgtgttc ccatagctac ttctttagct attttacgga agattgagcc gattcttctt
    14821 tgtaaatttc gcactcccgc ttcccggaca taataacgaa taatttcttg cagagctgct
    14881 ttggtaaaag ttattttttc ttttgccaag ccgttatttt ccttttcccg aggaatcaga
    14941 aagtgcttgg caatttcaat tttatcgttt tccagatagc tggtaaattc aattatctcc
    15001 attctgtctc ttaaggcggg aggaattgta tctaaggtat tggcagtggt aataaacagc
    15061 acttccgaaa gatcaaaagg cagattgata tagttatcca caaagctgtt attttgttcc
    15121 ggatcaagaa cttccaataa agcggaagaa ggatcacctc taaaatccct gcctacctta
    15181 tcaatttcat ccagcataaa aacaggattg gcagttccct gacgcttgat ttccattatt
    15241 attttacctg gcatagcgcc aatataggtt ctgcggtgac cgcgaatttc tgcttcatca
    15301 tgaattccgc ctaaggacat ccgaataaac ttgcgggaaa gagctctggc aactgatttg
    15361 ccaatagagg tctttcctgt tcctggagga cctacaaaac aaagaatagg tccttttaag
    15421 ttgcctttca atttctttac ggcaatgaat tccaggatac gttctttagc ttctttcaag
    15481 ccgtaatggt ctttttccag aatgcgttca atctttttca aatccaaacg gtctttgcta
    15541 tactctctcc aaggcagatt aacaatccaa tccagataat ttcttactac gctgtattcg
    15601 ctggaaacag gttgcatagt ggcaagacgc tctaattctt ctaatgcagt ttctttcaca
    15661 tagtccggaa ggtttttctg ttcaataagt acctgccatt tcataatttc tttgctaact
    15721 tcatctgttt cacccaattc tctgcgaatg gcatccattt gttctcgcaa ataataacga
    15781 cgctggtctt cactcatttc cagctgaata ttactgcgga tatgattttc caccttcatt
    15841 tgtttaatca gctccgccaa acaattgttt aaataacgga aacgctgttt gaggtcaatt
    15901 gtctccaata ttttttggcg gtcagaaata ggcaggtcta tatttccggc aataatatca
    15961 gccacccttc ctgcttgttt tatattacta agcccggtaa tcatttcccg gttgagaatg
    16021 ttgctttcct gagctatttt ttccagcaat tccaaagcta cggttctgta tgcttgtatt
    16081 tcggtatctt cttcaaattg ttccggaatt gcttctacat ctaccattat aaaaggattt
    16141 ttttgcacaa ttttttgcag tttaatgcgg gttgaaccct gcaggagcat actgattgaa
    16201 ccatcttgat tacgcagcat acgtaaaata gtaactgctg tgccaaaatt agccaattca
    16261 gttattcctg tatcgtcttt ttcctggtca aggaaaaaag ccatcaattt atcgttagcc
    16321 agtgcataat caatcaccag ctttgattct tcatccgaaa ctaccaatgg cataagcagg
    16381 tagggaaaca tcactacatt gctcatatgc agaaccggta aagttcgggg tattttagtc
    16441 gtgtaattca cttcatctcc taatgatttt ttacttttag tgtttgcgaa aaagatacaa
    16501 attcacattc attcttttca actatataat aatcaattca cagcaataag caaattttag
    16561 aaatatcacc cctgaacagc gatgtaaaga gcagttgcca gctttactaa attatcatcg
    16621 gtgtctttca aactttccga taatttccgg gctatgtttt tcatcactat ataaccggct
    16681 tggtaattat attcggcaaa attatcaaaa tccgctttag aaagtgtgta aagttcacaa
    16741 tcgctgagag caataatatt tgccattctg gttccattac ctaaaactcc gttctctcca
    16801 aaagtaggaa gggaatcggc acttagcttt gccaaaactt tttccgttaa gagctggtct
    16861 tcgtcaaagc cctttacaag gtctttagta acctgcacaa ttcctttcac caagatataa
    16921 atctgatcgc cttcggtatt ttctttgata attaccgtgc ctttattaac gagtaactgc
    16981 ttaaaacggg gttgcaaata agctaaagct tcggaattga catcataaaa taaaggtatt
    17041 tttgccagtg tttccagttc tatcataata agtcctttta aatcatcagg atggcataat
    17101 catttttttg cagttgaaca tccttctcag gtttccagcg aatgttgctt ttttcttccc
    17161 gaattttctt ttgggacttg gttagcgcat attgaataaa ttgatcaatt ccactaatat
    17221 cttccgtaaa aattgaagaa agctccagtc ccggttcttt ggttaataat cctaaaagta
    17281 aataaccgca ttcccgataa taataatcgg ataagtcacc aaaacttttt ccgataaaat
    17341 cggaaggaat tttctgcgtc tgcagatgat tttgtttatc ctgaaccagc tggcaaaaca
    17401 cagccaaata attattatca gctatgctat tcgccaaaat gtaaccccca atatcatccc
    17461 agataataat attccgaatt cccaaacgct gaagcataga acgattttct ggattcacca
    17521 gctgtacggt tatcttatca tttttggtta aataatgaac tgcatttgcc acaatgatac
    17581 tgtggtcatc agcaacagaa ggatctaact gttcatcagc caaaataata acctgagcag
    17641 atgtgtccac cgaagctcgt tttaaggtct cttcctgaat tgcgtcaccc ctaacgaatt
    17701 tcagggataa tgtaggaaag cgtgattcca accgctcaaa aaaatccggc gtggcattga
    17761 taacaataca aatatccatt tcggagacct gtttttccaa aagtgctttc agaaaaaact
    17821 cggcagtatt attccagcca caaatcacga tatgattatg tgttcttatc gttttcaaac
    17881 ccttagctcc tttcagccgg tcttctacaa aaagagaagc aatcagaccg gtaaaaaatg
    17941 ataatgaaga ataacctact ataatcaata taatgcctac caaccgacct tgaaaagtga
    18001 caggatattt atctccataa cctactgtgg ctatagaaac aattgcccac cagattccat
    18061 cccagaatga ttttatggta ttttcctgag ttcctttctg ttcaaaagcc caagccagca
    18121 aagccgaaat taaaagcaga atagctataa aggaacaaac ccataaaaaa cggataaata
    18181 atttttgatc ctcaccgcga atagacaagc ggtctgaaat tttccgaaag ggatgcagga
    18241 acagcttcat tttttatacg ataatcagga accaaaccat attcagaatg ccgattaaca
    18301 gattgagccc agcacaaata agtaacatcg gtttataaac ataacgcttg aaatagccat
    18361 agaaaaaatc cacactgaac tgaatgtcaa attcatcaat ccgatgttca atttgcagct
    18421 gttccactaa tctgggaact gtctttctta aaatgctttc tgctattgcg gtaaaggcat
    18481 ccacaatctt gtttctaacc aacagcttta actttgcagg taaaaaacgc cattcatcta
    18541 taaagcttgt tttttcccac aaatactgac gcaccttttt tatccaaccg taaagatagc
    18601 cgtcttttag gtaaggatga gccgcctgat caagataatc gtgtaaaatt gaacgcactt
    18661 tgtcaaacag ccaatccctt ttggcaacaa agaaacctgg agttaacgga atctttttcc
    18721 caaatagata cctggctttg cgattgaaca agagccaatt taaaagaagc accagaagtg
    18781 ttccaagagc tatgttccat aagataaaag tgagtgactt taagaaaatc atttaaaaac
    18841 ctcctccacc tcccccacca cctgaacgtc cacctccaaa acctccaaaa cctccaaaac
    18901 caccaaaacc gccacttccg ctactccagg aaccgctatt tcctccacct ccgcgtcctc
    18961 caaaaatact catccaaaaa agccattcca aaattctgcc acgagtagct ataattaaaa
    19021 taataaaaag aatgagaaaa gcaagcaaac ctacaccgcc tgaactttct ttcttctttt
    19081 gtgaatattc cgaaacgccg gttaaagtaa ttcctttttc tttggcaata gtggaaagca
    19141 acattagcga gccctgaata aaggcatcat cccatttttc gttacccttg gtgaggtagc
    19201 ttttcattgt ttgatagacc tcactgatat aggcatcggt taaatatcct tccgaaccgt
    19261 agcccacttc aaaacgcaat tttctttctt taagagctaa aagaactaaa ataccttcat
    19321 cccgtttact tcctattccc cattgttcat aaagtttaac cccgaaatct acaatatctt
    19381 ctcctccgat atcaggaaga gtaacaatta cgaaatccac atctgtcttt tctcgcagtt
    19441 caattgccca gtcattaatt ttattgcggg tttcagcact taaaatattg gcaaagtcat
    19501 ttacgaaacc caccggttta ggaacaacca cactaaacag taatgctgtt agaaaaacaa
    19561 acgctaaaac agcacagaac cgtttatatt tcattcagaa tatgccccat tttgttcttt
    19621 ttagttttta aatactcgcg gttgttttct tggggtttaa tttcaattgg cacccgttct
    19681 acaatttcca gtccgtaacc gcttaaacct actatttttt taggattatt agtcatcagc
    19741 cgcatttttc ttattcctaa atccttcaga atttgtgcac cgattccata gtcccttaaa
    19801 tcgggaggaa aaccaagttt gatatttgct tgaactgtat ctacaccttc atcctgaaga
    19861 tggtatgcct taattttatt cagtaaacca attcctcttc cttcctgacg catatacaaa
    19921 atcacaccct ttccttcttc cgaaatcatt tgaaaagcac gagccaattg ttctccacaa
    19981 tcacagcgca aactgaataa agcatcgccg gttaaacatt cggaatggac tcttaccaaa
    20041 ataggttcat cgcgctttat ttcacccatc actaaggcaa tatggtattc atccgaaata
    20101 tcactgatat aggttataat atcaaattga ccatactttg tgggcaattg tgctttggaa
    20161 acaggatgga caaggcgttc tttttttctc cgccagtgaa ttaacgcttc aatagtaata
    20221 attttcaggt tatgcttttt ggcaaaaggc agcaaatcat ccaaacgagc catttcgcca
    20281 tcttcgcgaa taatttcaca aatagcgcca acagggctca aaccggcaag tttagccaaa
    20341 tcaacagctg cttcagtatg acctgctctt cttaaaaccc cacctctttc tgcaattaaa
    20401 ggaaaaatat gacccggacg cataaacata gaggcattgg cttgcgggtc tgccaatgtc
    20461 ttaattgttt tagctctttc aattgcagaa attcctgttg tcgtgccttc aataacatcc
    20521 acggaaacag caaacttcgt gccttgacga tcactacttt tattagtcat caaaggaata
    20581 tccaaacggt tggctatttc tatatccaaa ggagcgcaga gcagaccttt gccttggctg
    20641 atcataaaat ttaccttttc cggagtaatc ttatcagcag ccattaaaag gtcaccttca
    20701 ttttccctat tttcgtcatc tacaacgatt agcatatcac ctttgcggat aatttctatt
    20761 gcttcttcaa tagaggcgaa aatagtttta tccacttctt gttctggatt agtcattata
    20821 tatttcctca ttcaaaatca ggttggcatc actggtatct gtgtcaatac taaaattatt
    20881 atccaaccag ttcaaagtat attgtatatc atcaggtatg ggagctgaaa cagatagagg
    20941 ataaccggtt atgggatgta aaaattccag tttccaggaa tgtaatgctt gtctgcgtaa
    21001 atggttcaac agcagttcgg taaccttttt tttcatattt tgcggtacca gggattgcac
    21061 atagcgtctg ctattatata ataagtcccc taaaatagga taacggatat gggcaaaatg
    21121 cactctaatc tgatgcattc ttcctgttgc cggatttatt tctaccaggg caaagtagtg
    21181 ccaggttttt attgttttat aatgagtgag tgcatagcgt ccttcatcgg taacgcagat
    21241 agttctggga tttttattac tgcgtgcaag ataggtctct atttctcctt tttcgggctc
    21301 gggaatccct gtagtaatag caaaataggt ctttttaatc tctcttctgg caaacatatc
    21361 ggaaagcttt gcctgggtaa gatcattttt agctattata atcagtcccg aagtgcctct
    21421 atccaagcga tgaacaattc ccggacggtt aaattctttt ccgctggaaa ggttttctcc
    21481 aaagcgataa agaatggcat tcaccaaagt tccttcggga tttccaaaac ctggatgcac
    21541 aatcattcct gcttttttat tgataatagc gaggtgttca tcttcataaa ccacatccaa
    21601 gggtatatct tgaggcaaaa cattcatcgg ttccggctcg ggaagattga taataacttc
    21661 atctccttcc ttaagaagat agcttttttt tacgggagta agattaacca aaacacgctc
    21721 ttcttctatg aggtgttcaa taaaagtgcg ggaataaagt tcctgtatct tcaattccgc
    21781 cagaaactta tccaagcgtt gcatttcctg taaagtatag attaccttca gtttatcttt
    21841 cattatcata aaattccagc acataaacag cgccggtaga aagacctttt ctgtttctca
    21901 ctatggaacc attaatttta accttgccca aatacactac atcttcaaca attatatcgt
    21961 aaatattatt gccatagcgc agggaatcta agataacgga aaaaatgcgg ttatggtaat
    22021 cgtttttcca aatcaattca ttcaaattta ccgtttctga aatcatcgga aagcgttttc
    22081 tgaggggttc attacagtag acaacatcac cattagctcc ggtaattaaa atactttgtg
    22141 gcagaaggtt aatcaaaagc tggaggcgtt gatgatgttc aaatatttta tctgcctttg
    22201 cctgatcaaa acggataatt attcccagca ttttatccag agcataaatc aattcctgaa
    22261 aatccttatc ctggtcataa atagagctat caatatcaat ctgatagttg ccattggaaa
    22321 tttcttctat cagctgttcc acttcacgaa ttgctttatg caggcggaaa ggtatgtagt
    22381 tgtaaagaat tacgatatac acaaaaatga taaagacgaa gataatcacg attgaatgaa
    22441 tatcgttagc aagagttgtt aagtcctgtg cccgagctac agaaaaaagc agggcaagag
    22501 tttgtaacac tatgatgcca aagagaatgg caatcaaaat atggtatcgt gctttaaaag
    22561 agcgtttcat tgtttctcaa tccttcttaa cggctgtttc atagatcagt ttatctatat
    22621 tgccttttgg ccctaataaa attaaaacat cgccttcgtt gataatatca ttggctccag
    22681 gcatatcatt cagccgtttt tctattacat tttgaccgtc ttccgtaaca gagagatagt
    22741 tgtatttgat agctataaca tttaccccgc gttttgtagg aagggcaagt tcctgtaacg
    22801 gcttacctac aaattcttta ggagcaacca tttccactac aatatgaccg ctggagagat
    22861 tgatatagtc caaaacatta cgggaaatca aggttttagc cagctggttg ccaactattt
    22921 cttccggcaa taaagtatgt tgaactccca gcattttcag aattctgcca tggagttccg
    22981 attcaatttt ggcatagata attcctacgc ctattttttt caggattgca gcagtgagaa
    23041 tgcattcttc aatattgctt ccaattccga taataacggc atccgcttca ttcagatttt
    23101 gcgatcttaa agctgcttca tcggtactat ccatacagac agcagaagta actatagaac
    23161 tgatttcatt taccaaatcc tgatctttat caatggcaat tacttccata ccgttttctg
    23221 tcaagatttt ggcaacagtc attccgaacc ggcctaatcc aattactgca tatcttgcca
    23281 tatttttctc cttatttcaa gtaggttaat ctaaccgatg gaaattcttt cttccgcata
    23341 agatatatgg ggttgtcttt tgcgaataga aatggcatat accagggtta gaggtccgat
    23401 tcttccaata tacattaata aagttatcag cagtttaccc gcataagata actgtgatgt
    23461 tattcccaaa cttaagccaa ctgttccaaa agcggaaaat gcctcaaaaa gtattttctc
    23521 aaaaggaaac ggctcaatga gcatcagaat aaaaataatc gttaaaataa tcgccaaagc
    23581 taatgttgcc aaagttgttg cttctcgggc attagcaaga gatattttgc gcttgaaaac
    23641 taccagatcc ctttttccgg taagcataga aaggacagaa agcattaaag tggaaaatgt
    23701 tgtggtcttt attcctcctc ctgtagaacc tggagaagcc ccaataaaca tcaataaaac
    23761 agttaacagt acagacgcgg aactgtatga accaatttta atcgtgttaa agccagctgt
    23821 ccttgcagtt acggattgaa accaggaact taaaaccctg ccagcaaccg aaaaaccttt
    23881 cattgaattg ttatattcac ctatataaag ccccaaaaaa cctgtaacta tcaatattgc
    23941 ggtagtggaa agaacaattt tggaatgcaa agctaactta cgaaccctcc tggaagaaaa
    24001 gaaataatga taaatatcta tcaggacggc aaaacccaat ccgcctaaaa taatcagaaa
    24061 agaaaaacta aaaattaccc aaggagaccc tacaaaaccg gcaagattat tactccacaa
    24121 agaaaaaccc gcattacaaa aagccgatat agaatgaaac aaagaataat atatcgcttg
    24181 acccgcaggc attagtttcg caaaagaggt aaaaagaaat aaggcaccta ttatttctat
    24241 tatcaaagta accaacacag tacttctaag taatttcaga acatcaatta caggggtctt
    24301 acctacaaca ttatgcatca agtttgttac atttaaagaa attctttgcc ccagcattaa
    24361 agcaaaggca gtagatatag tcataattcc taaaccgcca acttgaatta agagtaagat
    24421 tacgatttga ccaaacaaag aataataagt tcctgtatct acaactatca aacccgtaac
    24481 acaagtggca gatgtggctg taaacaaagc atctacaaag tttgtaatat aaccctcagt
    24541 agaagaagca ggtaacatca gcaaaattgt tccaattaaa ataacgcctg caaaactgaa
    24601 catcaaagaa acaggaggat tcctcatcag caatttgtat attttaccct caaaagctct
    24661 gaataaaatg aactggacga taaaccaggt ctgccggatt aaaaggaaaa agattacaaa
    24721 ctttgcctga taggtgagca gtaatgaacc caaaatcaac atcaccaaat caaaatagac
    24781 aaccgttttg ttttctttca cctcccggga aagcaccctg gaaagaatcg taagcactaa
    24841 tagaactaaa tttgccagag cagttataaa ctcaatagtc ggataggaac tggtgtaata
    24901 ttcaaatatg ggctccagaa aaagaacgaa aaaactccag aaagccagta tataggcaat
    24961 tagttcaatg tgtttaaaag ttcctgattt cttctctgcg ggcattttag tttattttgt
    25021 taccttattt tcaggtcgtt tattatttcc tgttgaatct cttcaattga tttgcgggca
    25081 gatatttctt tcagcaaacc cttttctgca aaatattcct tcaacgcgaa ggtctgttca
    25141 taaaatgcct taactcgttc ccgaattgct tctccggaat catccgtacg ctgaactaaa
    25201 ggggaaccgc atttatcaca aatcccttcc tgctttggtt ttttgcttaa aatattatag
    25261 gtctcgccac aagaactgca aagacggcgg gcttcaatgc gggcaattgc ttcttcttcg
    25321 cttagttcca aatagtaaac tcttctcaca ttataatttt ttaacagata ttccgcctgt
    25381 gccaaagtgc ggggaaaacc gtcaaaaact acaccagcac aatcagctgt taaagaagca
    25441 tttacaattt caaataccag ttcgtcctga accagttcac ctttggcaat aattgcctgc
    25501 acttttttcc cgagttccgt ttgtttatga acttcttccc ggaataaatc gccaatatta
    25561 atatgttgaa atccggttct ttctgccaga agttccgctt gagttccttt tccacttccc
    25621 tggattccca aaaagacgat tacatctaac attttttacc tcatttttta caaaaaaaag
    25681 caattacatt cttctgtcaa gcgaaatgaa aaatagcaag aggttaaggg gtcaagagga
    25741 caagaaaata agaggacaaa agggttttta ctaaatattt tcacttgtct tccaggaaaa
    25801 tactccccca gaactaattc acttacaacc gaacttatat cagtttacaa attgaaatat
    25861 ttggatttat aatagaataa gcccccgtaa tttgtattaa cctataatgc atacttacat
    25921 aaataattag ccaaaaatat catctcttcc ctaatagcat tgcttaccca ttgcttaccc
    25981 attacggatg gcattagcaa tgggtgagca atgctaaagt aatgcactta ggaaaaaatg
    26041 ggttgttctt ttaaataaat ataagtaatc agataatttc cccttggaaa aaccatagat
    26101 aaaaatactc ttaaggattg caagtatcaa atgtcatttt ttgaatgata aaccatttgt
    26161 catttctgcg caagcaggaa tccacacaag ataaataata tctatgcgtt ttccttccga
    26221 tattttataa aaaccgattt taacccggtg taaggggaga aaaaccactc aactacactt
    26281 ctatgaagaa aaaagttcc
//
LOCUS       DAOOZV010000024        25206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2120_length_25206_cov_59.213351, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000024 DAOOZV010000000
VERSION     DAOOZV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 25206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25206
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_25206_cov_59.213351"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1719
                     /gene="proS"
                     /locus_tag="PLW08_03890"
     CDS             196..1719
                     /gene="proS"
                     /locus_tag="PLW08_03890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPL51776.1"
                     /translation="MSKNNKTAITPTREENYAEWYQQVIKAADMAENSEVRGCMVIKP
                     WGYAIWENIQRALDKMFRETGHRNAYFPIFIPKSFFEKEAEHIEGFAKECAIVTHSKL
                     EADGKGGLQLAGELTEPYIVRPTSETIIGASFAKWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTTEFLWQEGHTVHETEKEANEETLKMLNVYATFARDYLALPVIQGEKTESEK
                     FPGAVTTYCIEAMMQDRKALQSGTSHFLGQNFAKASEIKFQNRKGEIEYAWTTSWGVS
                     TRLIGAIIMAHSDDNGLVLPPKIAPSHIAIIPIYRDAQEQKQVMAFAEKVQNRLKDLY
                     LDDVPLYTELDTRDMTSSERNWYWIKKGIPLRLEIGPRDIIQDSVMLARRDKEPQDKQ
                     SVKISDLTNYVLQTLVEMQQGYYQRALAFKDKWTIEIDDKEEFYRFFTPKNIEQPEIH
                     GGFASSHWCGKSSCEEKIKEDLKVTIRCIPFNTKEETGNCICCGKPSTRRVIFAKAY"
     gene            1721..2950
                     /locus_tag="PLW08_03895"
     CDS             1721..2950
                     /locus_tag="PLW08_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPL51777.1"
                     /translation="MNAAIITIGNEILMGKTINTNLAYLGSELAKQGISVEYALTVKD
                     EPNAIKQALQDTWGKYKIVITTGGLGPTEDDLTKSAISEFFGKKQHFDDNIWEHIQKM
                     FAFRKIPLPETNRSQAMVPDDFIPLRNDLGTAPGLFYRERDFLFFALQGVPSEMKHIF
                     ETHIKDIITQNCPDAKPLSIKTLRTYGIAESRLAELFTLSNLPEGVSLAWLPQIGRVD
                     LRFYGTDANKVEEAVKKALPVIKPFVWGYDEESPAEILLALLWKNIYTLSVAESCTGG
                     LVQKLITDIPGSSNSFLGGVIAYSNALKKNLLNVSDTVLETEGAVSETCALQMAEGIK
                     SLTKSTCSISITGIAGPDGGTAEKPVGTVCFGFIAAEKYWSKKQFFTGDREIIRIKAA
                     DFAILSLIQHLQGRNDN"
     gene            2947..4194
                     /gene="purD"
                     /locus_tag="PLW08_03900"
     CDS             2947..4194
                     /gene="purD"
                     /locus_tag="PLW08_03900"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPL51778.1"
                     /translation="MNILVIGSGGREHSIAECFAKSKQIKRVIVAPGNAGIALQYECA
                     NLQTQEEIYNFCVQNLIDLVFVGPEKPIADGLADYLNEKGVAVIAPVKFAAQLESSKI
                     FAKNLMQEKGVPTAAFKIAKTIPQALEHIDYFGFPAVIKNDGLAGGKGVFVVKNPAEA
                     QEALKKIHFSEKGVVVEEFLQGWEVSLFAITDGWEFKTTIFAQDHKQLYAGDKGPNTG
                     GMGAYAPVYEAEKYRKQIEEEIINPVLMAMRELGHLYKGILYCGLMITKEGPKVMEFN
                     CRLGDPEAQVVLPLLETDFVEICQAILQGEINLVELKFKDKTALGVVLASEGYPFAYN
                     KGFPLSLPADLSGIYFSGVEKGENGLVTSGGRVLCAVGMGKDLNEAREEAYSKVQQIT
                     FENRIFRQDIGLRKNTFYNKGDS"
     gene            4199..9280
                     /locus_tag="PLW08_03905"
     CDS             4199..9280
                     /locus_tag="PLW08_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51779.1"
                     /translation="MPMYYINRVSNLFRSLSCRKILFFCLSFCLPALIMAEPITLVSE
                     SQDILAINFELPEYELTKVTINGQNWERIVCSDGSYFSNEGFPQLIMFSTAIAVPVDG
                     DYSFSIKSSESKTLTNINIIPSSTLVIEGEEVNYNNKPDYKAYANRELYPVSLAQKGE
                     PAFIGNRKFIPLLIYPFQYRAQSKELIVHSKISITVYISGTKNATKNWQLNPNPLDNA
                     DPPFFLNENSSKTWRLEKTRDINYSAPKNSISSINEIQIIVDKEGIYKVSYHYLMDYI
                     NLMADSLQIVMNWTPANVDPRYLELTDEYGQVPIHFVGENDGVFNENDYFEFYGDKHK
                     GDTYYMDDYTSENVYTLKLIDHYGARMAVENGGLIESNLLNYIVPDAYEDTVHFEEQL
                     VSDKLGRGWSSSTPNFYREDVWFWKKINAPNLEIVPVELQYPKDTTTRFASARICLMG
                     LTYSETLTSGQYDHEASIRLNQAMVNSHTWIGQTEKIFENQNHISNTFLRHGTNNFYI
                     ALSGNTVMTDKEQVMLDWAEIKYWREYKTDQDYIKFTKPSNRPGGLYQFEVSGFSNPH
                     ISVYKIGSSIFNSAQIEPFNINGDAPWTVTIQDSVFSEAVRYFAVTENMKKTPKAIRL
                     NFPSDLKNPDNSADVIVIAPWQFTSVEGTLQLKSLWESEGHTVKIVDVQDIYDEFNAG
                     ITGAEPIRDFLRYAYNNWSPPQLSSVILLGEGVDDTRDNSPSRIYNLIPVKKTWTYKH
                     GATASDPWYGCIVGTDIVPDISIARISIWKEQQILDYAAKATAYRNNPQTSRLWNSHL
                     TFTSGGKITDNNDIFAQQSERIIRKTIPDEYRVTRVYTSTQTVSSDYFGGTFNLKDAI
                     NSGTQYVQFMGHGGGRIWADYNLFNFNDVATLNNQTYPVVLSLACYASAFDTNGMASI
                     SEALVLQPNKGAIGTAGFTGLGYLDHDETWGMAYCDALYKHNFANIGLANIFALARAY
                     TTIYSPAAIYALINGFAYLGDPLIKLKKPIKDIPVFADNYVLNPGDTLRVNATFPTGV
                     NAGRLFIMKESGKIVNVPYDLPVFPNGNWTATYVNTNPAANNYTRKIMVAGYSNTDEY
                     IGLSQFSVGRPNIMHHHLNPPEPAWSDSVLFTAKVFSPLPVNNIICKVRTDSVSTQIN
                     WLQLPMQRSEQDSTLYVTTHYLGKYPTGKEIFFKYVMETETGITESFLKNYIVRGPDL
                     FLKDIKLEQEGNSLVLKVLGTNIGDATSITTDLKLYTGTTESNLTLFSTQNYSPLEVG
                     ELRWDTIPLAGLPNANLILEARVNTTNAFSEWHFFINTNNYIRLKVPMNYFLVDSSSS
                     THNSIDNNLQCSIPPGLVPAGSQVLFAINTLNALPPLNQPDVQNILLNAPDGLTGNQH
                     SIPYEISFLGTGITDSLGVLNGGKKLFLSFNYSATDSLTQAQESANNFKIYRYNAKFQ
                     KWILIGSFMDQSQNKVSFEVNRTGIYSIFRNMDYTPPTIDVNVEDQEFTVGGYVAGNG
                     VISLLLSDTNGIDVIDNSISLFLDGVPIPEKDYVISINLENINRIPIKYQLSLGKGIH
                     ELKVQCRDLNGRPANRDIQFTVNDKFDVINLANYPNPVLGAGGEGAALDPKNEGRTRF
                     TYVLTDGADEVTIKIYTLSGRLVKTFKNLPVGVGYHEYPRSVYGWDCKDDKGYTLANG
                     VYFYRIIAKKGNKTIEKTQKMAILN"
     gene            9291..10301
                     /locus_tag="PLW08_03910"
     CDS             9291..10301
                     /locus_tag="PLW08_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51780.1"
                     /translation="MKKIYILFALTLICLTPLFAGRYAGDFMMIGSGVRALGMGGAFA
                     SLADDGSGIYWNPAGLSFLTDSEVSAMHCFLYNNLASYDHITYSQPLPNDVTICLNVT
                     RLTVSDIPHFDEKYLIGTNVDQRINNSLYHLPGVPDSKFSSYDDLYQFAFSKKIHYDA
                     NMGWLFFAVPFDFGFGGTVKLIKRSIWDRTGTGTGLDIGFKAKTDLAVIFDWEELGDL
                     HFGVNFQDIGGTDITWDTPSKISDEVLFNTKTGLAVVQPLPKLKSVVTLAYDYDYVYG
                     GTNHFGVDWEYDKKGNLRLGYYDNSFSAGATIKLYGVMLDYALITNPVGISNRLGLRV
                     RF"
     gene            10313..11092
                     /locus_tag="PLW08_03915"
     CDS             10313..11092
                     /locus_tag="PLW08_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51781.1"
                     /translation="MRKIIILTTLLLLALLFSCSGDDSPEKDKIPPTTPKLISHLGDT
                     GDDPIIVNGVTVSLNDDNNGIDTVPDGNWIKVPWEKFVDNDLSHVKVYRYSESDPEPI
                     LINTVPASEDYYLDQSSLVEREWYYYYIELYDASGNFSVSDTVSYAILAKSILSSPVE
                     GETINQDTAKLCWERGDSQTNKFRILLWDNLTGNLVFSYDYYYTPNEESPSPPEFPFP
                     RLNPRPPSGQVFRWRIDAFDDDPEHNLEMGSESKERTLIIQ"
     gene            11215..11700
                     /locus_tag="PLW08_03920"
     CDS             11215..11700
                     /locus_tag="PLW08_03920"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPL51782.1"
                     /translation="MKRLLLVFLLISFSLVLFSQQTINFTPSSNISAYTYEGIRSGVE
                     KLKMNNYILGQVYKPGLYTVPDDTDFLTLLSLAGGPKEDAKLTKIRIVRPSSEGDKVI
                     WVNFKKYLETGDPTLIPELKPGDTIIVSGTIFYAVSKVAKFLSDITIALGVYNIITGF
                     K"
     gene            11713..14001
                     /locus_tag="PLW08_03925"
     CDS             11713..14001
                     /locus_tag="PLW08_03925"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPL51783.1"
                     /translation="MLENENNQLPVQEEIKLSDYFRVILQYRYLVILVFLVALVATVY
                     YTARLPKIYSASTRILLEDANKQSDLMFLSTGGLGKNTLNNQIELIRSKPIMNLAWEI
                     MKKYPDWDTFPASQAADPASTLGRMKVESKRETDVLTISFESTNPTEAMAAVNSIAEA
                     IQQQNTQYARLEFTTIREFLETQLDAISRRLQSSENDLREFKNLNKLTQLSEETNKLI
                     EQASEVEAEYEAALTDQAVKAKSLDLLNRQLQEQDSLLVNVDNIIKTPYINELRKQIV
                     DTQSLITKLVTKNEYPLDHPQIQLLYREIENAKEKLDQEIRKLVNLAVTDDPLATRSN
                     LLTRIVQANIELEMARAKVDGLEQTKEMYNQRLIAIPNTELELARLTRNLMLDEKIHS
                     MMMEKYEDAKIAEQAKIGNIRIIDYAERPTVPIKPRVSMNILVGIIVGLGLGIGTAFL
                     VHSLDTKLRTLEDMENYVRLPIAGTIPVIHEAESKLQEFNDMINSATGENREQLTRSM
                     HYVMMQLVSHYAPKSPIAEAYRTLRTNILAHKGESSIAILITSSGPKEGKSTTMANLA
                     VTFAQMNSKVLLMDMDLRRPTLHNKFDLEKENGTSDFLIDPEVSVDQVIKPTGIQNLD
                     VITSGFIPPNPSELIASPRTDVLLEELKSRYDYVLVDSPPLIAVTDALILTKKVDMTY
                     LVVRCGFTDKGIIKRTKELMNNIEGRIDGIIVNGIYVQKYYSKQNYYYYYYYYYYYYG
                     EEVPQKQKRNVNRFLRKNKSIS"
     gene            13961..14782
                     /gene="ispE"
                     /locus_tag="PLW08_03930"
     CDS             13961..14782
                     /gene="ispE"
                     /locus_tag="PLW08_03930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPL51784.1"
                     /translation="MLTASYAKINLFLELIGKLPNNYHQVNTILCSIDLFDLLNYELI
                     ESPEIILTCNIPSLTSTSNLIYRVASYIKERFNVSSGIKIHLEKHIPVSAGLGGGSSN
                     AANCILALNEMWQLNLSEQQMHKIASQFGSDVNFFLEGGTAKGENRGEVITKLPSIFL
                     NNILLVNPGIEISSAEAYKLAYIPKKQEQRKFDPSNLIGSCFNRLESGIRSAYPVIDE
                     IINTISSFGAKVAIMSGSGSTCFGVFDSKNDLKACRQYFSQKGFWTYTTKTRETS"
     gene            complement(15487..16701)
                     /locus_tag="PLW08_03935"
     CDS             complement(15487..16701)
                     /locus_tag="PLW08_03935"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HPL51785.1"
                     /translation="MKNILCNIVNPISPEEIQFLPKQVISIEESKISAITSLPEFQGR
                     IDEDRSNEYALPGFIDLHTHLSQYYIRGLYEPALLPWLNKYVFPEEERSKNIEYAEKL
                     SRDFFSAMLKTGTTTCVIYTAPFFSACDMAFEIAKETGIRALIGMTMMDMNCPENLKQ
                     NSHKTLEESILLYEKWHGKNAKLDYIFTPRFAPTCSLELMKEVAKYAIEHNAWIQTHL
                     SENKEEIEMVKEIFGFKNYTEVYQKAGLLTQHSIFAHCIHLSDEEIKMLAENKCKIAH
                     CPDSNFFLKSGEFPLQKIEEAGIEYGLGSDVGAGTSLNMLYHTKMMNYRQGDYPVLPA
                     KALYHITLGSAKLLGVDKIIGSLEIGKEADIVFLKPPLNYPLKNNGISQLVFCGQEFS
                     LTETLVAGRNKE"
     gene            complement(16698..19367)
                     /locus_tag="PLW08_03940"
     CDS             complement(16698..19367)
                     /locus_tag="PLW08_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HPL51786.1"
                     /translation="MRYINLFIFILLVFSCYAITNEEINFLCAKEQFQILAKYDETIQ
                     AKVLYGDEEDLQTVLNYSHRANLPELAKQCHKRLALNYNSLEDALQWLMLCETEEVDS
                     LTFYNDFSAITEAITDSLDSAVWLYYTKRCSEEELYAKIATTKKYNTIIEAMAKDLID
                     EISIERNDSLAFNLLNEFETKYPHSRYRSIALYYKLYHFANRKNWQELIKALPQHTNL
                     DPVSAYIASLFLLSPTFRKDFEGEENLLELAEQYLTLALSDCEQTLLYDIYSADDWKA
                     RVLQQQAKLLFYRIIEPYGLFGDELDIPMLEKNKLKQQQELLALLAQVQFSNNNRGEQ
                     AEKHFWTAKALLLTGKKTDKQKAAEHLTQCLISGSPRNRYDIEAMALITKLHTDLKIK
                     EEPLKWMRKMMNYKGICFEDKSENAGLNGKGYTRVALADYNSDGFTDILFNGKYLYRN
                     EGKMKFIEVTDSAGLANLSSSGGIFADFNKDGLLDLVSYSHSAEGRGDQLLKNMDNIR
                     FVNVNERAGDIDDTYPTEAVAWIDIDQQGYPSLYSANYEIWLKRSGFPDYFWKNDGGY
                     FTDKSDAYGFREPPYTDNPGLPGRGVAPADFNNDGKQEIYVTNYRLSRNFCWQQIDTL
                     FVDVAALYGIAGTYKNGYYGHSIGADWGDYDNDGDLDLFVANLAHPRYIDISDISMLL
                     RNDGLTYRIVEKDTIYYWQFTDVTEQTGIKYDELHSDPLFFDADNDGYLDLFITSVYE
                     NERSYLYHNKGDGTFEDVTWLAGARVYNGWGNACADLDRDGLQDLIVGSGNGSKILHN
                     GTITKNKGLFIKPYWKNDIVLLENKAENFAKVPNSPAFGTRVEITVKRLFHKKQKFIR
                     ELCSAKGTGSQNAPELHFGIGKAKVIKIRKVEQ"
     gene            complement(19364..20245)
                     /gene="hslO"
                     /locus_tag="PLW08_03945"
     CDS             complement(19364..20245)
                     /gene="hslO"
                     /locus_tag="PLW08_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPL51787.1"
                     /translation="MTKDYILRGTVHNNNFRVFAVNSTNCVQTARDLHDLSPIATLLL
                     GRMISAAAMLSWDLKDEESEITMRIDCEGDIQGAIVICTQNGFLRGYTKNPRLFYATR
                     EDNFKVGKAVGKGTLTISKDKKGTRSYFSTIELINGEIAEDLAYYYQQSEQIPTAVNL
                     GILIDKEAKVRSAGGFIIQQLPYADKKIADVINENIAQTPNLSDLMDMGLSIPEILSR
                     FVFKGLQWQINEEKEIAYRCNCSYELFSRALLLLGKEELQSLKEGIKPVCLYCNKEYN
                     FSSEDIQSLIEQLQEQK"
     gene            complement(20238..21731)
                     /locus_tag="PLW08_03950"
     CDS             complement(20238..21731)
                     /locus_tag="PLW08_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPL51788.1"
                     /translation="MKRNYLIILLLFCLKLLAGAQFIKGELYLEINLTASPQLTITPK
                     GKNANITITEMDGLISRNFDSAITINIMNPETLVHYAILDFTEIVYAAHDDEQAVVRD
                     FFAWENSRLKIKKELQHFLPESFTTEEEAKQYASKQGIPIDKIKEVQLLNSTVQIQDA
                     TGNLCYMETPLKIHSEGEIYFNRNSYDYSGDFILKTIKKQIILNQLLPLETYLAGVVQ
                     NEIGDNAPAEALKAQIVAARTHALTLLLNNKHKTDGYDLCNSTHCQVYKGKYLLNEQI
                     LQAVYECASEVLFYNGNLAETTYHSCCGGKTEAADIIWKGKPIDYLKGVTCLNAVNSF
                     DLSKEKDARAWIDKPLAEEEMSSWERGALSWHKSISLNQLSKNAGLSSISRIEILQRG
                     SSGRITKVKLIGNETISLNSEYQIRQVFGNLLSSFFYIEGSYQIANGTVIIIPKPNIK
                     IKGRGAGHGVGMCQVGALRMARKGTKYEEILQHYYPGTTINKDWIDD"
     gene            complement(21834..23870)
                     /gene="metG"
                     /locus_tag="PLW08_03955"
     CDS             complement(21834..23870)
                     /gene="metG"
                     /locus_tag="PLW08_03955"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPL51789.1"
                     /translation="MKYLVTSALPYANGKLHIGHLAGAYLPADIFVRYLRLKGEDVIY
                     ICGTDEHGTPISITADKEGITPQEVVNYYHNSIKKAFEGIGIEFDNFSGTSRPPHYKL
                     AADFFLQLYNKGYIKPKNTLQFYCEKDKRYLPDRYVEGICPLCGFEDARGDQCDKCGQ
                     IYETTTLIEPKCKICGSTPVIKETKHWFLLLEGFREELKRWLATKDYWKENVRNFILN
                     LLDQGLVERSVTRDLSWGVPVPLPEAKGKVLYVWFDAPIGYISSTVEWAEKIGQPEKW
                     KEYWLNPETRLIHFIGKDNIIFHSLIWPSMLMGQDTTYCLPYDIPANEFMNLEGKKIS
                     TSKNWAIWVDEFIQDFDSEYLRYYLAVNAPERQDSDFSFKDFQNKINIELNNTLGNLA
                     NRVFAFANKNFEGKITACALSETANKTIAEADKLLKEIEENYLDYQVKKNTRLIMDIA
                     RLGNRYFDENKPWAQLKENRDATNETLYVCANLLAKISVAFSPIIPRSMRYLRQMMNL
                     DDNFKFSEAYNLLGDIVLNEVKPLFRKIEDKEIETQIAKLHSQAIINEGGKSDFDTPE
                     IAPLKELISYDDFAKLDLRLAKVLTAERVPQTDKLLKLKVDIGIEQRELIAGIAVDYE
                     PNNLIGKTVLMLVNLEPRKIRGITSQGMILAANSKGKLLLLIPDGEGIPGAVVQ"
     gene            complement(23867..25048)
                     /locus_tag="PLW08_03960"
     CDS             complement(23867..25048)
                     /locus_tag="PLW08_03960"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPL51790.1"
                     /translation="MAIIVKKFGGTSVGNIDLIRNIAHRLTSEYHKGEGLVLVVSAMA
                     KTTDELFSLAYGISKHPSRRELDMLLTAGERISMSLLSLALMEEGIPSISFTGSQSGI
                     ITDEKHGNARILKVNAFRIQEELEKGKVVIVAGFQGVSTSKEITTLGRGGSDTSAVAL
                     ACYLKANKCEIYTDVDGVFTADPKLVEKPKLLRQISPELMLTLCYNGSKVLHPRAAEY
                     ALKYGVEVEVKSSFTFAPGSLITNKKTENNEENNMEERKVTAIAHKNALLRYTFPLND
                     KVLKTLSDWYYEIYKSFVNEGLLELFVESKYETEVDYFLQERDIKPISKKGDFAFVNL
                     IGLGLGHDPAFLATVMENCQNFNILRSFNNEQSIELLLPDQNCAAAVKTLHKIYVEEQ
                     V"
BASE COUNT         7711 a         4816 c         4724 g         7955 t
ORIGIN      
        1 aatgacaaat ggctgaacat tcaaaaattg atattatttg ggaaaaagca gtatatgcct
       61 gtctttcctt tttacattga caacaatggc actattcagg aattggattt ctaatggtaa
      121 tatagctaat atattccttt tggttttgca taataagcat ccgaactatt cagacacaaa
      181 tattggagaa aagtgatgag taaaaataac aaaacggcaa taacacctac ccgggaagaa
      241 aattatgccg aatggtatca acaggttata aaagcagctg atatggctga aaatagtgaa
      301 gttcgcggtt gtatggtaat aaaaccctgg ggctatgcaa tttgggaaaa tatccagcgt
      361 gcactggata aaatgtttcg ggaaacggga catcgtaatg cttattttcc tattttcatt
      421 ccgaaaagtt tttttgaaaa agaagctgaa catatagagg gctttgccaa agaatgcgct
      481 attgtaacac acagtaaatt agaagcagat ggaaaaggtg gtttacaatt agccggtgaa
      541 cttaccgaac cttatattgt gcgtcctacc agtgagacaa ttatcggcgc atcttttgct
      601 aaatgggtaa attcctatcg ggatttgcct ctcctgataa atcaatgggc taatattgtc
      661 cgttgggaaa tgcgcacgcg tcttttttta cgcactacgg aatttctttg gcaagaaggg
      721 catacagtgc atgaaacaga aaaagaggca aatgaagaaa cattaaaaat gcttaatgta
      781 tacgctacct ttgcgcgtga ctatcttgcc ctgcctgtta ttcaaggcga aaaaaccgaa
      841 agtgaaaaat ttcccggagc tgtaacaaca tattgtattg aagcaatgat gcaggataga
      901 aaagcactgc aatctggaac ttctcatttt ttgggccaga attttgccaa ggcatccgaa
      961 atcaaatttc aaaaccgcaa aggagaaata gaatatgcct ggactacttc ctggggtgtt
     1021 tctacgcgtt taataggagc tataattatg gctcacagcg atgataacgg acttgttttg
     1081 cctccgaaaa ttgctccttc ccacattgct attatcccta tttacagaga tgcacaagaa
     1141 caaaaacagg tgatggcttt tgcggaaaag gttcaaaacc gactaaagga tttatattta
     1201 gatgatgttc ccctttatac tgaattggat acaagagata tgaccagcag tgaaagaaat
     1261 tggtattgga taaaaaaagg cattcctttg cgcctggaaa taggtcctcg ggatattatt
     1321 caggattctg ttatgcttgc cagacgcgat aaagaaccac aagataagca gtcagtaaag
     1381 atttccgacc taacaaatta tgttcttcaa actctggtag aaatgcaaca gggatattat
     1441 caacgagctt tggcttttaa agataaatgg acgatagaaa ttgacgataa agaggaattt
     1501 tatagattct ttacacctaa aaacattgaa caaccggaaa ttcatggtgg ttttgccagt
     1561 tctcactggt gtggaaaatc atcctgcgaa gaaaaaatta aagaagacct gaaagtgact
     1621 attcgctgta tcccttttaa cactaaagaa gaaacgggaa attgtatctg ctgtggtaaa
     1681 ccttctacac gcagagtaat ttttgccaaa gcatactaaa atgaatgcag ctattattac
     1741 tatcggtaat gaaattctga tgggtaaaac catcaatacc aatctggcat atttaggttc
     1801 ggaacttgcc aaacaaggaa tttctgtaga atatgccctt accgtaaaag atgaaccaaa
     1861 cgctataaaa caggcactgc aagatacatg gggaaaatat aagatagtaa taactaccgg
     1921 tggtttgggt cctacagaag atgatttaac caaatccgca atttctgagt tctttggtaa
     1981 aaagcaacac tttgacgata atatttggga gcatatccaa aaaatgtttg ctttcagaaa
     2041 aataccatta cctgaaacca atcgctctca ggcaatggtt cccgatgatt ttattccttt
     2101 aagaaacgac ctgggaactg ctcccggatt gttttatcgg gaaagggact ttttgttctt
     2161 cgccttacag ggtgttcctt ccgagatgaa acatattttt gagacacata tcaaagatat
     2221 tattacccaa aattgtcctg atgccaagcc actatctata aaaacattaa gaacttacgg
     2281 catagctgaa tcccgactag ctgagttatt tactctttct aacttgccgg aaggtgtttc
     2341 tttagcttgg cttcctcaaa ttggaagagt tgacttgcgc ttctatggaa ccgatgctaa
     2401 taaagttgaa gaagcggtta aaaaagcact gccggttatt aaaccttttg tttggggcta
     2461 tgatgaagaa agtcccgcag aaattttatt agcgcttttg tggaaaaata tctacacttt
     2521 aagcgttgcg gaatcctgta ccggtggctt ggtgcaaaag ctgataacag atattcctgg
     2581 ctcatctaac tcctttctgg gtggtgtgat agcatattcc aatgccttga aaaaaaatct
     2641 gctgaatgta tccgatacag ttttagagac agaaggagcg gttagtgaaa cctgtgcttt
     2701 gcaaatggct gaaggaataa aatcattgac aaaatctacc tgctcaattt ctataacagg
     2761 gattgcaggt cctgatggtg gaactgcaga aaaacctgta ggaacggtgt gttttggctt
     2821 tatagccgct gagaaatatt ggtctaaaaa acaattcttt accggggata gggaaattat
     2881 ccggattaaa gcagctgatt ttgctatcct ttctttaatt cagcacttac aaggcaggaa
     2941 tgataattga atatcttagt aatcggcagc ggaggcagag aacattctat tgcagagtgt
     3001 tttgctaaaa gcaaacagat aaaaagggtt attgtggctc ccgggaatgc aggaattgca
     3061 cttcaatatg aatgtgcaaa tctgcaaaca caagaagaga tatacaattt ttgtgttcag
     3121 aatttaattg accttgtctt tgtcggaccc gaaaaaccca tagcagatgg tttagctgat
     3181 tatttaaatg agaagggagt tgccgttata gctcctgtta aatttgccgc ccaattggaa
     3241 agcagcaaga tttttgccaa aaatttgatg caggaaaaag gtgttccaac tgccgcattc
     3301 aaaatagcta aaaccattcc tcaagcatta gaacatattg actactttgg ctttccagcg
     3361 gttattaaaa atgatggttt agctggagga aaaggagttt tcgtggttaa aaatccggct
     3421 gaggcgcaag aagcattaaa aaaaattcat tttagcgaaa aaggagttgt tgtagaagaa
     3481 tttttacagg gttgggaggt ctctttattt gccattacag atggctggga gtttaaaact
     3541 acaatttttg ctcaggacca taaacaactt tatgccggtg acaaaggtcc caacacaggt
     3601 ggaatgggtg cttatgctcc agtttatgaa gcggaaaaat accggaaaca gattgaagag
     3661 gaaataatta atcccgttct tatggcaatg cgcgaattgg gtcatcttta taagggaatt
     3721 ctctactgtg gactgatgat tactaaggaa ggaccgaaag ttatggaatt caattgccgg
     3781 ttaggagatc ctgaagcaca ggttgtttta cctttactgg agacggattt tgtagaaatc
     3841 tgccaagcca ttttacaggg tgagataaat ttggtggaac taaaattcaa agataaaacc
     3901 gcattaggtg ttgttttggc ttccgagggt tatccttttg cttacaataa aggttttcca
     3961 ctttctttac ctgcagacct ttccggaatt tatttctccg gagtggaaaa aggtgaaaat
     4021 ggacttgtga cctctggtgg acgtgtttta tgtgcagttg gaatgggaaa agatttaaat
     4081 gaagccaggg aagaggcata cagcaaagtt caacagataa cctttgagaa cagaattttc
     4141 cgccaagata ttggattacg taaaaatacc ttttataaca agggagatag ctaatattat
     4201 gccaatgtat tatattaacc gggtgagtaa cctgtttcgt tccctttcct gcagaaaaat
     4261 tttattcttc tgccttagtt tttgcctgcc tgccttaatt atggcagagc caataactct
     4321 tgtttctgaa agtcaggaca tacttgctat taattttgag cttcctgaat atgaattaac
     4381 caaagttaca ataaacggac agaactggga aagaattgtt tgtagtgatg gctcttattt
     4441 cagtaatgaa ggttttcctc aattgataat gttttctacg gctatagcag ttcctgtaga
     4501 tggcgattac agcttcagta ttaaaagtag cgaatccaaa acgctgacga atataaatat
     4561 catcccttcg tcaactctgg taattgaagg tgaggaagta aactataata ataaaccgga
     4621 ctacaaagct tatgccaatc gtgaactcta tcctgtttct ctggcgcaga aaggtgaacc
     4681 tgcttttata ggtaaccgca agttcatacc tttgctgatt tatccttttc aatatagagc
     4741 acaaagcaaa gagctgattg tgcactccaa aatatctata actgtctata taagcggaac
     4801 aaagaacgca actaaaaact ggcaactgaa tccaaatcct cttgataatg cagacccgcc
     4861 ttttttcttg aatgaaaata gttctaaaac ctggcgtctg gaaaaaaccc gggacataaa
     4921 ttattctgca cctaaaaata gtatttcaag cattaacgaa atccagatta ttgtagataa
     4981 agagggaatc tataaggtta gctatcatta cttgatggat tatataaatt tgatggctga
     5041 ttctttgcag atagtgatga attggacacc tgcaaatgta gacccgcgtt atctggaatt
     5101 aactgatgaa tatggacagg tgcctattca ctttgtaggc gaaaatgacg gtgtcttcaa
     5161 tgaaaatgac tattttgagt tctatggtga taaacataaa ggtgacactt actatatgga
     5221 tgattataca agcgaaaatg tatataccct gaaactgatt gatcactatg gagcaagaat
     5281 ggctgtggaa aatgggggtt tgattgaatc caatcttctg aactatattg ttcctgatgc
     5341 ttatgaagat acagttcact ttgaagaaca actggtaagc gataaacttg gtaggggctg
     5401 gagttccagt accccaaatt tctatcgcga agatgtttgg ttctggaaaa aaatcaatgc
     5461 tcccaatctg gaaattgttc ccgtggaact gcaatatccc aaagatacta caacccgctt
     5521 tgcttctgcc agaatttgtt taatggggtt aacttattcc gaaacactaa ccagcggaca
     5581 atatgatcat gaagcatcta ttcgtttgaa tcaggcaatg gttaattcac atacatggat
     5641 tggccaaacg gaaaagatat ttgaaaatca aaatcatatt tccaacacct ttttaaggca
     5701 tggaaccaat aacttctata ttgccctttc cggtaatact gtaatgacgg ataaagaaca
     5761 ggtaatgctg gattgggctg aaattaaata ctggcgcgaa tataaaactg accaggatta
     5821 tatcaagttt acaaagccct ccaatcgtcc tggtggttta tatcaatttg aggttagcgg
     5881 tttcagcaat cctcatattt cggtttataa aatcggctcc agtatcttca attctgctca
     5941 gatagagccg tttaatatta atggtgatgc tccttggacg gttactattc aagatagtgt
     6001 tttttccgaa gcagtcagat attttgccgt aactgaaaat atgaaaaaaa ctcccaaagc
     6061 aatacgcctc aattttccca gcgacctgaa aaatcctgat aatagtgctg atgtaattgt
     6121 tattgcaccc tggcaattta cttcagtaga aggaactttg cagttgaagt ccctctggga
     6181 atccgaaggg catacagtaa aaattgtaga tgttcaggat atctatgatg aatttaatgc
     6241 tggcattaca ggtgctgaac caattaggga ctttcttcgt tatgcttata acaattggag
     6301 tccacctcaa ttaagcagtg ttattctttt gggtgaagga gttgatgata ccagggataa
     6361 cagtccttcc cgaatatata atctcattcc ggttaaaaag acctggactt ataaacatgg
     6421 tgctacggca agtgacccct ggtatggatg tattgtagga acagatattg tccccgatat
     6481 atctattgca agaattagta tctggaagga acaacaaatt ttggattatg ccgctaaggc
     6541 aactgcttat cgtaacaatc cacaaacctc tcgtctttgg aacagtcatt taactttcac
     6601 ttctgggggg aaaataactg ataataatga tatctttgct caacaatcag aacgtataat
     6661 ccgtaaaact attcctgatg aatatagagt caccagggtt tatacttcca cgcaaacggt
     6721 tagttcagat tactttggcg gaactttcaa tctgaaagat gctattaatt ccggaactca
     6781 atatgttcaa tttatgggtc atggcggggg tagaatttgg gcggattata acctcttcaa
     6841 ttttaatgat gtagcaacgc tcaacaatca gacctatcct gtggttttat ctcttgcctg
     6901 ttatgcttct gcttttgata caaacggaat ggcttctatc agtgaagcat tagtccttca
     6961 gcccaataaa ggagctatag gcactgcagg ttttactggt ctgggttatt tagaccatga
     7021 tgaaacctgg ggtatggcat actgtgatgc tctttataaa cataattttg ccaatatcgg
     7081 tctggcaaat atttttgctt tagcccgcgc ttatactact atctattcac ctgcagctat
     7141 ttatgccctt atcaacggtt ttgcctattt aggtgatcct ttaatcaaat tgaagaagcc
     7201 cataaaagat atacctgttt ttgcagataa ctatgtgctg aacccgggtg atacattacg
     7261 cgttaatgct actttcccca ccggtgttaa tgcaggccgt ctgtttatta tgaaagagag
     7321 tggaaagatt gtaaatgtcc cttatgattt acctgtcttt ccgaacggga attggacagc
     7381 tacttatgtt aatacaaatc ctgcagccaa taactacacc cgtaaaatta tggttgccgg
     7441 ttattccaat actgatgaat atatagggct ttcccaattt agtgttggta gacctaatat
     7501 tatgcatcat catcttaatc ctccagaacc tgcctggtcg gattctgtct tgtttactgc
     7561 caaggtcttt agtcctttac cggttaacaa tattatctgc aaggtcagga cagatagtgt
     7621 ttctactcaa attaactggc ttcaacttcc aatgcagagg tctgaacaag atagcacttt
     7681 gtatgtaact acccactatc ttggtaaata tcctactgga aaagagatat ttttcaagta
     7741 tgttatggaa actgaaacag gtattacaga gagtttccta aaaaattaca ttgttcgtgg
     7801 tcctgatctg tttctgaaag atattaaact ggaacaagag ggaaattctc tagtcctgaa
     7861 agttttagga accaatattg gcgatgctac ttctataact acagatttaa agttatatac
     7921 cggaacaact gaaagtaatc ttactctgtt ttccactcag aattattctc ctttggaagt
     7981 aggagaactc cgttgggata ctattcctct tgccggctta cctaatgcca atcttattct
     8041 ggaagcaaga gttaatacta ctaatgcttt ttctgaatgg catttcttta tcaataccaa
     8101 taactatatt cgcttgaaag ttccgatgaa ttatttcttg gtagatagtt ccagttcaac
     8161 tcacaacagt atagataaca accttcaatg ttcaattcct ccaggtcttg ttcctgcagg
     8221 tagccaggtt ctttttgcta taaatacatt aaatgcctta cctcctctta atcaacctga
     8281 tgtccaaaat attttgctta atgctcctga cggacttacc ggaaatcaac attccattcc
     8341 ctacgaaata agtttcttgg gaaccggaat taccgattct ttaggtgttt tgaacggagg
     8401 aaaaaaactt tttctttcct tcaattacag cgctactgat tccctaactc aagcacagga
     8461 aagcgctaat aacttcaaaa tatatcgcta taacgcaaaa ttccagaaat ggattttaat
     8521 aggcagtttt atggatcaaa gtcaaaataa agtcagcttt gaagtgaatc gcacaggtat
     8581 ctacagtatt tttcgcaata tggattatac tcctccaacc atagatgtta atgttgaaga
     8641 ccaggaattt actgttggcg gatatgttgc cggtaatgga gttatttctc tcttgctttc
     8701 cgataccaat ggtattgatg taatagataa ttctatctct ctgtttttag atggtgttcc
     8761 cattccggaa aaggactatg taatctccat caatctggaa aatatcaata gaatccctat
     8821 taaatatcaa ttatccttag gaaagggaat acacgaatta aaagttcaat gtcgtgattt
     8881 gaacggccgt cctgcaaacc gggatatcca atttaccgtt aatgacaaat ttgatgttat
     8941 aaacttagca aattacccga atcccgtttt aggagccgga ggtgaaggtg ctgctttaga
     9001 cccgaaaaat gaaggcagaa ctcgtttcac ctatgttctt accgatggag cagatgaagt
     9061 tactatcaag atttacacac tcagcggccg cctggtaaag acctttaaaa accttccggt
     9121 tggagttggt tatcatgaat atccccgttc agtttatggt tgggattgca aagatgataa
     9181 gggttacact ttggctaacg gggtttactt ttaccggatt attgccaaaa aaggcaataa
     9241 aaccattgaa aaaacccaaa aaatggcaat tctgaattag ggagaccaaa atgaaaaaga
     9301 tatatatcct attcgcactt accctgattt gcttaactcc actttttgca ggacgttatg
     9361 ccggcgactt tatgatgatt ggttccggag tacgtgcttt aggtatgggt ggtgcttttg
     9421 cctctcttgc agatgatggt tctggaattt attggaatcc tgctggtctc agttttctta
     9481 ccgattccga ggtttccgct atgcattgtt tcctctacaa taatcttgct tcttacgatc
     9541 atataactta ttctcaaccc ttgcccaatg atgtaactat ttgtctgaat gttactcgtt
     9601 taacggttag tgacattccc cattttgatg aaaagtatct catcggcact aatgtagacc
     9661 agcggataaa taacagctta tatcatttac ctggtgttcc cgatagtaaa ttttccagct
     9721 atgatgatct ctatcaattc gctttttcca agaaaattca ttatgatgcc aatatgggtt
     9781 ggttgttttt tgccgttcct tttgatttcg gttttggagg aacggtgaaa cttattaaaa
     9841 ggtctatatg ggatagaacc ggaacaggaa ccggacttga cattggtttt aaagcaaaga
     9901 ccgaccttgc cgttattttc gattgggaag aactgggaga tttacatttc ggtgtcaatt
     9961 ttcaagatat tggtggaaca gatatcactt gggatactcc tagtaaaatt agtgacgaag
    10021 ttctctttaa tactaaaacc ggcttagcag tagttcaacc cttacctaaa ctgaaatccg
    10081 ttgttacctt ggcttatgat tatgactatg tgtatggtgg aactaaccat tttggtgtgg
    10141 actgggaata tgataaaaaa ggtaatttgc gtcttggtta ttatgataat agtttcagtg
    10201 caggtgctac aataaaactt tacggcgtta tgcttgatta tgctctaatc acgaatcctg
    10261 tcggtataag taaccgtttg ggattacggg ttagatttta aggagaataa aaatgcgcaa
    10321 gataattatc ttaactacac tcttattgct cgctttgctc ttttcttgtt caggagatga
    10381 ttctcccgaa aaagataaaa ttcctcctac tacacctaaa ctaatttcac accttggaga
    10441 tacgggtgat gatcccatta ttgtaaatgg agtaacagtt tccctaaacg atgataataa
    10501 tggcattgat actgttcccg atggcaactg gataaaagtt ccctgggaaa aattcgtaga
    10561 taatgattta agccatgtaa aggtctaccg ctattccgaa tccgaccctg aaccaatatt
    10621 aattaatact gtcccagctt cggaagatta ctatctggat caaagttcat tggttgaaag
    10681 agagtggtat tactattata ttgaactcta cgatgccagc ggcaatttta gcgtttcgga
    10741 caccgtttcc tacgctattt tagctaaaag tattctcagt tctccagttg agggggaaac
    10801 tattaatcag gacacagcaa aattatgttg ggaaagaggc gatagccaaa ccaacaaatt
    10861 tcgtatcctg ctttgggata atctaaccgg taatctggtt ttttcttacg attattatta
    10921 tactcctaat gaagaatctc cttcaccacc agaatttcct tttcctcgtt taaatccacg
    10981 acctccaagt ggccaggttt ttcgttggcg aatagatgct tttgatgacg atccggaaca
    11041 taatttggaa atgggttccg agtcgaaaga gcgaaccttg ataattcaat agggataaca
    11101 gtccaacctt gaaaaagcat ataaaaaccg gtctggacat aatgaagtta cagaaaaatc
    11161 ttgacttgaa cagcgatgtt cagaatgtta cgcaaattaa atagagagga taatgtgaag
    11221 agattactgt tagtgtttct actcatcagc ttttccctgg ttttattcag ccagcagacg
    11281 attaatttta ccccgtcttc aaatatttct gcttatactt atgaaggtat ccgcagcggg
    11341 gtggaaaagc tgaaaatgaa taattatatc ttgggtcagg tctataaacc cggcttatat
    11401 actgttcctg acgataccga ttttttaacc ctgctttcct tggctggagg tcctaaagaa
    11461 gatgccaaac ttactaaaat aagaattgtg cgtccttctt ccgaaggtga caaggttatc
    11521 tgggtaaatt ttaagaaata tctggagaca ggtgatccaa cccttattcc ggaattaaaa
    11581 ccaggggaca caatcattgt atcaggaacc atcttctacg ccgtatctaa agtggcaaaa
    11641 ttcctctctg acatcactat agctctgggc gtttataata tcataactgg atttaaataa
    11701 ggagggtatt caatgctgga aaatgaaaat aatcaactcc ctgtccaaga ggaaataaaa
    11761 ctttccgatt attttagagt aatcctgcaa tatcgttatc tggtaattct ggtattcctt
    11821 gtagcattag tagctacggt atattacaca gcccgtttac ctaaaattta ttctgcttct
    11881 acccgcattt tattggaaga cgcaaataag caatccgacc tgatgtttct ttctacaggc
    11941 ggtttaggga aaaacacttt aaataaccag attgaactta ttcgcagcaa acccataatg
    12001 aatcttgcct gggaaattat gaaaaaatat cccgattggg atacttttcc tgcaagtcag
    12061 gcagctgatc cggcttccac tttaggtaga atgaaagtag aatcaaaacg ggaaactgat
    12121 gttctcacca ttagttttga atctactaat cctactgaag caatggctgc tgtaaattcc
    12181 atcgcggaag caattcaaca acaaaatact caatatgctc gattggaatt tactactatc
    12241 cgtgaatttc tggaaactca attagatgct atttcccgtc gtctgcaaag ttctgaaaat
    12301 gatttacggg aatttaagaa cctaaataag cttactcaat tatccgagga aacgaataaa
    12361 cttattgaac aagcatcaga ggtagaagct gaatatgaag cagcgttaac tgatcaggca
    12421 gtaaaagcca aatccctgga tttgctaaac cgtcaattac aggaacagga ttctctgctg
    12481 gtgaatgtgg ataatattat aaagaccccc tatatcaatg aactgcgtaa gcagattgta
    12541 gatactcaat ccctcatcac caaattagtt acaaaaaatg aatatccctt agatcatcct
    12601 caaattcaac ttctttacag ggaaattgaa aacgctaagg aaaaattaga ccaggaaatc
    12661 agaaaactgg taaatttggc tgtaactgac gatcctcttg ctaccagaag taacctatta
    12721 accagaatag tccaggcaaa tatagagctg gaaatggctc gtgccaaagt tgacggcttg
    12781 gaacaaacca aagaaatgta taatcaacgc ctgattgcta ttcccaatac cgaactggaa
    12841 ctggcaagat taacccgaaa cctgatgctg gatgagaaaa ttcacagtat gatgatggaa
    12901 aagtatgaag atgccaaaat tgctgaacag gcaaaaatcg gaaatattcg catcattgac
    12961 tatgcagaac gtcctacagt tccaattaaa cccagggttt ctatgaatat tctggtaggt
    13021 ataattgtcg gattgggttt aggaatcgga accgcctttt tggttcattc tctggatact
    13081 aaattacgca ctttggaaga tatggaaaat tatgttcgtt tacctattgc cggaactatt
    13141 cctgttattc acgaagcgga atccaaactt caggaattta acgatatgat taattctgcc
    13201 acaggagaaa atcgtgaaca actaacccgt tctatgcatt atgtaatgat gcaactggtt
    13261 tcgcattatg cccctaaatc acccattgct gaagcgtata gaactttgcg taccaacatc
    13321 ttggcacata aaggtgaaag tagtattgct attctgatta cttcttccgg tcctaaagaa
    13381 ggtaaatcca caactatggc caatttggct gtcacttttg ctcagatgaa cagtaaagtt
    13441 cttttaatgg atatggactt acgccgccct acattacata ataagtttga tctggaaaaa
    13501 gaaaatggca ccagtgattt cctgattgat cccgaagttt ctgttgatca agttataaaa
    13561 ccgaccggta tccaaaatct ggatgttatc accagtggct ttattcctcc caatccttct
    13621 gaactaattg cttctccaag aaccgatgta ttgctggaag aacttaaatc ccgttatgat
    13681 tatgtccttg tggattcacc acctttaatt gccgttacgg atgctcttat tctcactaag
    13741 aaagttgata tgacttatct tgtcgttcgt tgtggtttta ccgataaagg tataatcaaa
    13801 cgtaccaaag aattgatgaa taatattgaa ggtagaatag acggcattat tgtgaatggt
    13861 atctatgtcc aaaagtatta cagtaaacag aattattact actattacta ctattactac
    13921 tattattatg gcgaagaggt gccccaaaag caaaagagga atgttaaccg cttcctacgc
    13981 aaaaataaat ctatttcttg agctgattgg aaagctcccc aataactatc atcaggtcaa
    14041 tactatactg tgcagtattg acctgtttga tttattgaac tatgaactta ttgagtcccc
    14101 tgaaataatc cttacctgca atattccttc tctgacttca acttcaaatc ttatttatcg
    14161 ggttgcttct tatataaaag aacgctttaa tgtttcttca ggcatcaaga ttcatttgga
    14221 aaagcatatt cctgtttcag ccggtctggg aggtggaagc agtaatgccg ccaattgtat
    14281 tcttgcttta aatgaaatgt ggcagcttaa tttatccgaa cagcaaatgc ataaaattgc
    14341 ttctcagttt ggcagtgatg tcaatttctt tttggaagga ggaacagcta aaggagaaaa
    14401 caggggagaa gtgatcacaa aacttccttc tatcttcttg aataatatcc tgttagttaa
    14461 tcccggaata gaaatttcca gtgctgaagc ttataaactt gcctatattc ctaaaaagca
    14521 ggagcagcgt aaatttgatc cttccaacct aatcggctcc tgcttcaatc gtctggaaag
    14581 cggtatcaga agtgcttatc ctgtaattga cgaaattatc aatacaatca gcagttttgg
    14641 agctaaagta gctataatga gtggaagcgg ctcaacttgt tttggcgtat ttgatagtaa
    14701 aaatgatttg aaggcgtgcc ggcaatattt tagccaaaag ggtttctgga cttatactac
    14761 caaaacacgg gaaacatcat aataaaaaaa cttctcacag attacacgga tttcacagat
    14821 aaagtttagc taccgataat cccgaaaacc cggaaattca ggagttgtta ctaccgaaat
    14881 aaatattgtc caaaatctat aatcataaat cataagtgcg atatacaatt ccattttatc
    14941 acaactgcaa tattagagtt aaatacattt tccctctttc aagaaagata agttttcgct
    15001 ttcttgacta tctaaagaca aagtaacatt caagtaacaa ccaagtaacg aaacaactgc
    15061 agtgttactt cagtgttacg gagtcgttac ttaagcgtct atataacaag ctattagctg
    15121 tttctaatgg taatggacag aaaataggaa taattatatg tgacttatat aggttggcta
    15181 aaaattagaa agattttgag atagcgaagt agcaaggtag taatatattt atcatattat
    15241 attacagtta tataatatca ttatttgaat aaatattctt ttgtccttac ggcggaagca
    15301 tgaatcccta caagttctga gaaaataact gcctttgtaa tttagtaact attttataca
    15361 atttgcaaat ttgttgggca ggaagaactt atggcatcag gtatttagat cgttataagt
    15421 tatatgcccc ataaaaattt tctccccagt aaatgttatc agggtgcaaa tgagtcctta
    15481 cttaaatcat tctttatttc ttccagctac aagtgtttcc gtaagagaaa attcctgtcc
    15541 gcaaaaaaca agttgtgaaa taccgttatt ttttagaggg taattcaaag gtggtttcag
    15601 aaagacaata tccgcttcct ttcctatttc caaagaaccg attatcttat ctacaccaag
    15661 aagtttggca ctacctaatg ttatatgata aagtgccttg gcaggtaaaa caggataatc
    15721 gccttgacgg taattcatca tctttgtatg atagagcata ttcaaagagg ttccggcacc
    15781 tacatcacta ccaagaccgt attcaatacc tgcttcctca attttttgca aagggaattc
    15841 tccacttttc aggaaaaaat tggaatccgg gcaatgcgct attttacatt tattttctgc
    15901 caacattttt atttcttcat cactaagatg aatgcaatgg gcaaaaatac tgtgttgagt
    15961 tagcaaacct gctttttgat aaacttcagt ataattctta aacccgaaga tttccttcac
    16021 catttctatt tcttctttgt tttcagaaag atgggtttga atccaggcat tatgttctat
    16081 tgcatattta gcaacctctt tcatcagttc cagtgaacaa gttggagcaa aacgcggggt
    16141 aaagatataa tccagtttgg catttttccc gtgccatttt tcatagagca gaatactttc
    16201 ttccagtgtc ttatgactgt tttgtttaag attttctggg cagttcatat ccatcatcgt
    16261 cattccgatt aaagctctaa ttcctgtttc ttttgcaatt tcaaaagcca tatcgcaggc
    16321 agaaaaaaag ggtgcggtat aaataacgca ggttgtagtg cctgtcttta gcattgcaga
    16381 aaaaaaatct cggctcagct tttcggcata ttcaatattt ttagagcgtt cttcttctgg
    16441 aaaaacatat ttattcagcc agggtagtaa agctggctcg taaagtcccc gaatataata
    16501 ttggcttaaa tgcgtgtgta agtcaataaa cccgggcaaa gcatattcgt tagacctgtc
    16561 ttcatctatt ctcccttgaa attcaggtaa ggatgtaata gcagatattt tactttcttc
    16621 aatgctgata acctgtttcg gtaaaaattg tatctcttca ggggaaatag gattaacgat
    16681 gttacaaagg atgtttttca ttgttcaact ttcctgatct tgattacttt agctttgcca
    16741 atgccaaaat gcagttctgg agcattctga gaaccggttc ctttagcact gcaaagctcc
    16801 cgtataaatt tttgtttttt atgaaaaagc cgttttacgg ttatttctac cctggtgcca
    16861 aaggcaggac tattaggaac tttggcaaaa ttctctgctt tattttccag taagacaata
    16921 tcgtttttcc aatagggctt aataaaaaga cctttattct ttgttattgt cccattatgc
    16981 aatatcttag atccattgcc actgcctaca ataaggtcct gtaaaccatc tctatctaaa
    17041 tctgcacagg catttcccca accgttataa accctggctc cagcaagcca ggttacatct
    17101 tcaaaagtgc catcaccttt attatgatag agatagctcc gttcattctc ataaacggag
    17161 gtgataaaca aatcaagata gccatcatta tcagcatcaa agaaaagagg atcgctatgc
    17221 aattcgtcgt attttattcc tgtttgctca gttacatcag taaattgcca atagtaaata
    17281 gtgtcttttt ccacaatcct ataagttaag ccatcattcc tcaggagcat actgatatcg
    17341 gaaatatcta tataacgggg atgagcaagg ttagcaacaa acaaatccaa atcgccatca
    17401 ttatcataat caccccagtc cgctccaatg ctgtgaccat aatatccatt tttatatgtt
    17461 cctgctatgc cgtaaagagc ggctacatca acaaaaagag tatctatctg ttgccagcag
    17521 aaattacggc tcaaacggta gttggttaca tatatttcct gttttccatc gttattaaaa
    17581 tcagcagggg caactcctct tccgggtaaa ccgggattat cggtataagg aggttcgcga
    17641 aagccgtagg catcactttt atccgtaaaa tagccaccat cgtttttcca gaaataatcg
    17701 ggaaaaccgc tccgcttcag ccagatttca taattggcag aatagagtga agggtaacct
    17761 tgttggtcaa tatcaatcca ggcaacagct tctgtaggat aagtatcatc aatatctccg
    17821 gctctttcat tcacattcac aaaccttata ttatccatat ttttcagcaa ctggtcacct
    17881 cttccttcag cagaatggct ataggaaacc aaatctaaaa gtccgtcttt attaaaatcg
    17941 gcaaaaatgc ctccactgct actcaaatta gccaaaccag cgctgtccgt tacctctata
    18001 aacttcatct tcccttcatt tctataaagg tatttgccgt tgaaaagaat atccgtaaaa
    18061 ccgtctgaat tatagtccgc taaagcaact cgtgtataac ctttgccgtt aagacccgca
    18121 ttttcgcttt tatcttcaaa gcagattccc ttatagttca tcattttgcg catccatttt
    18181 agcggttctt cttttatttt aaggtcggta tgtagttttg ttatcagtgc cattgcttct
    18241 atatcatatc tattacgggg tgaacctgaa atcaaacatt gggtaagatg ttctgccgct
    18301 ttttgtttat ctgttttctt tcctgtcagt agtaatgctt tagctgtcca aaaatgtttt
    18361 tctgcttgtt cgccacgatt attgttactg aattgaactt gagcaagtaa tgcaagtaat
    18421 tcctgttgtt gtttcagctt atttttctcc agcatcggga tatccaattc gtcaccaaaa
    18481 agtccgtagg gctcaattat cctataaaac agcaattttg cctgttgttg taaaaccctt
    18541 gctttccaat catcagcact ataaatatca taaagcaagg tttgttcgca atcggacaaa
    18601 gccaatgtta aatattgttc tgccagttcc agcaggtttt cttcaccttc aaaatccttt
    18661 ctgaaagtgg gggagagcaa aaacagagaa gcaatatatg cggaaacagg gtctaaatta
    18721 gtgtgttggg gtagggcctt tatcagttct tgccaatttt tacggttggc aaaatgataa
    18781 agtttatagt ataaagcaat agaacgatag cgggaatggg gatatttcgt ttcaaattca
    18841 ttcaacagat taaaggcgag agagtcgttg cgttcaatac tgatttcatc aatcaaatcc
    18901 tttgccattg cttcaatgat tgtgttatac tttttggttg tggcaatttt agcatatagc
    18961 tcttcttcac tgcatctttt tgtgtagtaa agccaaacag cactatccaa agaatcggtt
    19021 attgcttccg tgatagcact aaaatcatta tagaaggtta aggaatcaac ttcttccgtt
    19081 tcacagagca tcagccactg taaagcatct tccaaagagt tataatttag cgctaaacgc
    19141 ttatggcatt gttttgccaa ttctggtaag tttgctctat gggaataatt caaaactgtt
    19201 tgcaggtctt cttcatctcc atacaaaact tttgcctgaa ttgtttcgtc gtatttcgca
    19261 agaatctgaa attgttcctt agcgcataaa aaattgattt cttcattggt aatggcataa
    19321 cagctgaaga caagtagaat aaagataaac aaattgatat atctcatttt tgttcctgca
    19381 gttgttcaat taagctctgg atatcttcag agctaaaatt atattcctta ttgcaataga
    19441 ggcaaacggg tttgatgcct tcctttagtg actgcagttc ctcttttcct aaaagcaata
    19501 atgccctact gaatagttca tagctgcaat tacaacgata ggcgatttct ttttcctcgt
    19561 taatttgcca ctgaagtccc ttgaacacaa aacgggaaag aatttcggga attgataatc
    19621 ccatatccat caagtcgctg agattgggag tttgtgctat attttcatta atcacatcag
    19681 ctattttttt atccgcataa ggtagctgtt gaataataaa acctcctgca gagcgcactt
    19741 ttgcttcttt atcaattaag attcccaaat ttaccgctgt aggtatttgt tctgattgtt
    19801 gatagtagta agccaggtct tcggcaattt ctccgttgat aagttctata gtgctaaaat
    19861 aagatcttgt tcctttttta tctttgctga tagttaaagt tccttttcct actgctttgc
    19921 ctaccttaaa gttatcttct ctggttgcat aaaagaggcg gggatttttt gtatagccac
    19981 gcagaaagcc attttgcgta cagattacaa ttgcaccttg aatatcacct tcgcaatcta
    20041 ttctcattgt aatttcactt tcctcatctt tcaagtccca ggaaagcatt gctgctgctg
    20101 aaatcattct tcccaaaagc aaagtggcaa ttggagaaag gtcatgtaaa tcgcgagcgg
    20161 tttgcacaca attagtacta ttaacggcaa aaacccgaaa attattgtta tgaactgtcc
    20221 cgcgtaaaat gtaatcctta gtcatcaatc caatccttgt ttattgttgt tcccggataa
    20281 tagtgctgta aaatttcctc atattttgtt ccttttcttg ccattcgcaa agctccaacc
    20341 tgacacattc caactccatg acctgcacct ctgcctttaa tttttatatt tggcttgggg
    20401 ataattatta cagtaccatt agcaatttgg taagaacctt caatataaaa gaacgacgaa
    20461 agcaggtttc cgaaaacctg gcggatttga tattcgctat ttaagctaat tgtctcattt
    20521 ccgatcagtt ttactttagt gatgcgtccg gaagagccgc gctgaagaat ttcaattctg
    20581 ctgatggaac ttaaacctgc attttttgac aattgattaa gcgaaatgga tttatgccag
    20641 gaaagcgcac cgcgttccca ggaactcatt tcttcttctg ccaaaggttt atctatccac
    20701 gcgcgagcat ctttttcttt gcttaaatca aaagaattaa ctgcattgag acaggtgaca
    20761 ccttttaaat aatcaatcgg tttacctttc cagataatat cagctgcttc cgtttttcct
    20821 ccacagcaac tatgataggt tgtttcagca agatttccgt tataaaacaa aacctctgaa
    20881 gcacattcat aaacggcttg caggatttgt tcattcagca aatattttcc tttgtaaacc
    20941 tggcagtgag tggaattgca taaatcgtaa ccatcggttt tatgtttgtt attcaatagc
    21001 aaagttaggg catgagttct tgctgcaact atttgtgctt taagtgcttc tgcgggagca
    21061 ttgtcaccaa tttcattttg cactactccg gcaagatagg tctctaaagg caataattga
    21121 ttcaagataa tctgtttttt tatggtcttg agaataaaat caccgctgta atcatagcta
    21181 tttcggttaa aatagatttc tccctcggaa tgaattttta agggtgtttc catatagcaa
    21241 agatttcccg tagcatcttg aatctgaaca gtggaattaa gtaattgcac ttctttgatt
    21301 ttatctatag gaatgccctg tttagaggca tattgttttg cctcttcttc cgtagtaaaa
    21361 ctttccggca gaaagtgttg aagctccttt ttaattttca agcgggaatt ttcccaggca
    21421 aaaaaatccc tgactaccgc ttgttcatca tcatgggctg cataaacaat ctctgtaaag
    21481 tccagaatag cataatggac aagggtttcc ggattcataa tgttaattgt gatagcggaa
    21541 tcaaaattgc gggatattag tccatccatt tccgttatag ttatattggc atttttccct
    21601 ttgggagtaa tagttagttg aggagaagca gttaaattga tttccaggta gagttcacct
    21661 tttataaatt gcgctccggc aagcaattta agacaaaata gcagcaaaat tatcaggtag
    21721 ttacgcttca tttctcttta tgctatcaaa aagaggttgt tattcctgtg gaagcaagaa
    21781 tccagataaa attgagtaaa ataaacacat tttcaagatg aataggatat aaattattgc
    21841 acaactgcac caggaattcc ttctccgtca ggaattaaaa gcagtaattt tcctttggaa
    21901 ttggcagcca gaatcattcc ctgcgaagtt attcctctaa tttttcgcgg ttcaagattt
    21961 accagcatta aaacggtttt gccaattaaa ttatttggtt cgtaatcaac tgcaattcca
    22021 gcaattaatt cgcgttgttc aatgccgata tctaccttta attttaacag tttatccgtt
    22081 tgaggaactc tttctgcggt taatactttg gctaagcgca aatccaattt ggcaaagtca
    22141 tcataactga ttaactcttt cagcggagct atttccggag tgtcaaaatc cgatttgcca
    22201 ccttcattta ttattgcttg tgaatgcagt ttggcaatct gtgtctcaat ttctttatcc
    22261 tctattttcc tgaaaagcgg tttgacttcg tttaaaacga tatctcccaa taaattatat
    22321 gcttcgctga acttgaaatt atcatccaaa ttcatcatct gccttaaata ccgcatagac
    22381 cttggtataa ttggactaaa ggcaacggat atttttgcca gtaaattagc gcaaacataa
    22441 agtgtttcgt tagttgcatc tctattttct ttcaactgtg cccagggctt gttttcgtca
    22501 aaatagcggt ttcccaagcg agctatatcc attatcagac gggtattttt cttcacttga
    22561 tagtccagat aattttcctc aatttctttt aagagcttat ctgcttcagc tattgtctta
    22621 ttagccgttt cacttaaagc gcaggcagtt attttaccct caaaattctt gttggcaaag
    22681 gcaaaaaccc ggttggcaag gttgcccaaa gtattattca gctctatatt tatcttattt
    22741 tgaaaatcct taaaactgaa atcggaatct tgtctttcgg gagcgttaac tgccaaataa
    22801 taacgcaaat attcactgtc aaaatcctga atgaattcgt ctacccaaat tgcccaattc
    22861 ttactggtag atattttttt cccttccaga ttcataaatt cattggcagg aatatcataa
    22921 ggcaggcaat aggtagtatc ctgtcccatc agcattgagg gccatataag cgaatgaaag
    22981 ataatattat cttttccgat aaaatgaatt aaacgggttt caggatttaa ccaatattcc
    23041 ttccattttt ccggttgacc tattttttct gcccattcca cagtagatga aatatatcct
    23101 atgggagcgt caaaccaaac ataaagaact ttgccttttg cttcgggaag aggaacggga
    23161 actccccagg ataaatccct ggtaacggag cgttcaacta aaccctggtc aagcaaattc
    23221 agaataaaat tccgcacatt ttctttccaa tagtcctttg tggcaagcca gcgtttaagt
    23281 tcttcgcgaa atccttccag tagcaaaaac cagtgtttgg tctctttaat tacgggagtt
    23341 gaaccgcata ttttacattt cggttcaatc aaggttgtag tttcgtaaat ttgaccgcat
    23401 ttgtcacact gatcacctct tgcatcttca aaaccgcaaa ggggacaaat tccttccaca
    23461 tatctatcgg gaagatagcg tttatctttt tcacagtaaa actggagcgt gtttttgggt
    23521 ttgatataac ccttattgta taattgcaga aagaagtcag cagcaagttt ataatgaggg
    23581 ggacgggaag ttccgctgaa attatcaaat tcaataccaa taccctcaaa tgctttttta
    23641 atactattat gataatagtt tactacttcc tgaggtgtaa ttccttcttt atcagctgtt
    23701 atggatattg gagtgccatg ttcatccgtt ccacaaatgt aaataacatc ttcacccttc
    23761 aaacgcaaat agcgaacaaa aatatctgct ggtaaataag ccccggctaa atgtccgata
    23821 tgcaattttc cattagcgta aggaagggcg ctggtaacta aatatttcat acctgttcct
    23881 caacataaat tttatgcagg gtttttaccg ctgcggcaca attttggtct ggcagtagaa
    23941 gctctatgct ttgttcgtta ttaaagctcc gcaggatgtt aaaattttgg cagttttcca
    24001 ttacggttgc caaaaaagca gggtcgtgac ctaagcccaa tccgataaga tttacaaagg
    24061 caaaatcgcc ttttttgctg attggcttta tatctctttc ctgcagaaaa tagtccactt
    24121 ccgtttcata tttgctttct acaaaaagtt ccagaagacc ttcattgaca aagcttttat
    24181 aaatttcata ataccagtcg ctaagagttt ttagcacttt gtcattaagc gggaaagtat
    24241 atctcagtaa tgcattttta tgagcaatag cagttacttt gcgttcttcc atattatttt
    24301 cctcattgtt ttctgtcttt ttattggtta tcagcgaacc gggagcaaaa gtgaaagatg
    24361 attttacttc tacttccaca ccgtatttaa gagcgtattc cgcagctcgg ggatgcaaga
    24421 ccttagagcc attgtaacaa agagttaaca tcagctccgg gctgatttgt cttaataatt
    24481 tcggcttttc cacaagcttg ggatcagcag taaaaactcc gtccacatct gtataaattt
    24541 cgcatttatt tgcctttaaa taacaagcta aagcaactgc agaagtatca gaaccaccac
    24601 gtcctaaagt agttatttct ttactggtgc taacaccttg aaaacccgct acaataacta
    24661 cttttccctt ttccagttct tcctgtatac gaaaggcatt cactttcaaa atccgggcat
    24721 taccatgctt ttcatcggtt attattccgc tttgagaacc agtaaatgaa atagagggaa
    24781 ttccttcttc cattagggct aacgacaata aactcatact gatccgttca ccagcagtca
    24841 ataacatatc caattcccga cgcgaaggat gcttggaaat cccgtaagct aaagaaaata
    24901 gttcatccgt tgttttagcc atcgccgaaa caactaaaac aagaccttct cctttgtggt
    24961 attcactggt taatctatga gctatgttac ggattaaatc aatgttacca actgaagttc
    25021 cgccgaattt tttcactatt attgccatat ctttctttat ttcaagattt gtagttgctg
    25081 tcaaggaaaa aaatctcgct ttcttaatag catttcttta gcattacgga ctcattgcta
    25141 atgccattag taatgggtaa gcaatgagta agcaatgcta ttaaggaaga aaatggaatt
    25201 atattt
//
LOCUS       DAOOZV010000025        24845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2166_length_24845_cov_53.376926, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000025 DAOOZV010000000
VERSION     DAOOZV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24845
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_24845_cov_53.376926"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2759)
                     /locus_tag="PLW08_03965"
     CDS             complement(<1..>2759)
                     /locus_tag="PLW08_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gingipain R"
                     /protein_id="HPL51791.1"
                     /translation="YLHSVYGVSHPTQAQDFAAYCNLMGDPTAMVYVGTPNSFIVSAP
                     STIPSGSANIRIGATNNNGESVEGAFVTLTTTSSLQLTGITDASGFVNIDLPAGLSQN
                     LVLTVAKDDYIAYMSSITLNTGGGIVYDSFVLDDDTTTGNGDGTLNAGEEVNLYVTLK
                     NTSSSTLFINGRVSTTDSYVTLIEYDRLEFGDIVAGGLGENITPIVFSVSPACPDQHI
                     ITLVLTAEGAGNNWTINIPLTVRGGNLEIQSYTFVGITGNIITPGLSSPFTFSLKNTG
                     ISTLHNVYGNLQSLSNYFVVQDAEGYFGNIAPNSTVTNSSNSFTVFAWDSSINGMVIP
                     LILTLSNANGYTQTLHLSITIGTTTVHDPLGQDAYGYFIYDQTDTAYPLCPSYQWIPI
                     APAEGGSGTLLSLTDPGNTSDEGDDTSAIAIQTVNLPFTFTFYGRQYTQASICSNGFI
                     AFGATQNADWRNWRLPGAGGPNPMIAVFWDDLQLGTGSGVYTYYNSAQHYYVVEWYNI
                     ISGYNGSSLETFQAILYDPVYYPTITNDGQIKLQYKVFNNIDQGSGDVYPHGNYCTIG
                     IKDHYGTDGLEYTFNNTYPTAASPLSNEKALLISTPEIISGTPHLTIAQTTVIDPNSN
                     NHLEPGETAQLQILLRNNGLSTAENVTGVLSCSDSYVTINNATANYGNIAGEAQGLPQ
                     SNFNLSVSPSCPGGHRINFTLALSGSGLNWNYSFSLDVYVPILEFGNYTVLDSNGDND
                     GILDPGETVNLSVQLLNTGEVASPVGTATIFTSTEGINIITGTAQFAPISGNGNTTLV
                     FNLSANASVAVGTLANLFCSAAAGTYSASTNIILEIGAPLEITIGTGTGTQTYPFDRW
                     YNYSAHEAIYLASEFGVAGAIKSLAYYKAQGVEINPIESVSIYMKQTPNTTLSSGNYS
                     LDGYT"
     assembly_gap    2760..2859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2860..4489)
                     /locus_tag="PLW08_03970"
     CDS             complement(<2860..4489)
                     /locus_tag="PLW08_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51792.1"
                     /translation="MKSSQFFLLLIITILLLPIALSGNTNGENNPFKIKEINDSEIRI
                     QFTLPQWEIEQINVKNEIRKKVKVQETPYLFIDEEETLPIFSTMIAIPNRGGVDLLVS
                     NSSKSSINEFTADFDAALNRENQQGRFTDIHYPAANVVISEPQILRDFRVVNLNIYPF
                     QYDRINRKLLVSENLDIKLTFNSRPSINELNSSSFISCSFDSIYRGLILNYESLRQTR
                     EIDYRNPVMLVIYGNYTDATYLSKINEFVTWKKQRGFIVNAVSTGVTGTTNTNIKSYI
                     QTAYNNPATRPDYIVLIGDTDGTIAVPSNNNYVDYQYTWLAGSDNLGDVMIGRISVTS
                     TADLDVILAKIISLEKNIGQHPTDWLNRMVLVGDTGDSGISTIYTNRYIRDISEEINP
                     AYTYTEVYGSSPSNTTINNAINQGVMCYNFRGWINMNSWPSTMSSLYNSYKLFHAVFI
                     TCSTGSFGGSSQPSTTEQVIRFGTAASLGGAITAIGMATSSTHTPMNNCLDVGIFHGI
                     YNLGMREMSSAMLYAKLYLHSVYGVSHPTQAQDFA"
     gene            complement(4745..5689)
                     /gene="arcC"
                     /locus_tag="PLW08_03975"
     CDS             complement(4745..5689)
                     /gene="arcC"
                     /locus_tag="PLW08_03975"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPL51793.1"
                     /translation="MNKTAVLALGGNAIIKAGEKGTITQQFANTRESLSGIVELIKRG
                     YHLSITHGNGPQVGNLLRQQEAGEKEGIPQLPLGVLNAATEGTMGYMIEQSLQNKLHK
                     SGIKKRVITIISQVVVDKNDPSMLNPTKFVGSTYYTAQQAEELHQKLGWILKEDSGKG
                     FRRVVPSPYPIQIIPAATIKELVDRGEIVIAVGGGGIPIYIEEDGTYEGVDAVIDKDF
                     ASSLLALEIKADLFVILTGVEKVAINYGKENQQDLDRMTVAEAKRYFAEKQFPAGSMG
                     PKIQAAIDFLERGGSEVLITSIEKIVDAFEGKTGTRIV"
     gene            complement(5831..7165)
                     /locus_tag="PLW08_03980"
     CDS             complement(5831..7165)
                     /locus_tag="PLW08_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPL51794.1"
                     /translation="MKRKVIIMGAAGRDFHNFNVFFRDNKDYEVVAFTATQIPGIDDK
                     KYPSALAGKLYPEGINIYPESELKNLIKKHKVDLVVLAYSDLPFEVVMHKASLVNACG
                     ADFMLMGKDNTTIKTSKPLVTVCAVRTGCGKSQTTRAVVKALKAKGLKVVSIRHPMPY
                     GDLVKQKVQRFAKLEDLKKYNCTIEEMEEYEPHIMMNSVIYAGVDYEAILKEAEKEAD
                     VIVWDGGNNDIPFYTSDKEIKIVVVDPHRPGDEISYYPGETNVFLADVIVINKIDSAD
                     FDDINEVRENVRFINPKAQIIEAASPLFVDHPEMIYGKKVLVVEDGPTLTHGEMTYGA
                     GIVAAEKYGCADLVDPRPWAAGEIKQTYEKYPEIGTLLPAMGYSSKQIKDLEKTINSA
                     ECDSVIIATPIDLRRLIKINKPACQVRYELQEIGVPTIADVLKNFGKKSSKK"
     gene            7485..7572
                     /locus_tag="PLW08_03985"
     tRNA            7485..7572
                     /locus_tag="PLW08_03985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7519..7521,aa:Leu,seq:cag)
     gene            7769..9109
                     /locus_tag="PLW08_03990"
     CDS             7769..9109
                     /locus_tag="PLW08_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPL51795.1"
                     /translation="MERKIFILDTNVLIHNPQALFSFEDNRVVLPIVVIEEIDQFKKG
                     VDEKSRNARQVGRYLDALRKKGKLQEGVPTENGGTIQVTVNKEITNTASELLFLDRND
                     NLIISTALYFKEKYPQSIVTLVSKDVNVRVKADCVGINAENFETDTIKYEEFFTGWEF
                     LDLEPEQYRELEEKGYINNNFGDFFPNQFVRVSVPDKPDQYKTLRYHYGRNQLYVINH
                     YTGQQVFGIKARNFEQEMALDILLDDSIKLVSLSGKAGTGKTLLAMAAGLQKVVEEKK
                     YSRLVVSRPISPLGKDLGYLPGTKTEKFNPWMQPIYDNMDILLSSHTDKSQEGSPSRK
                     KTDINDLMDYGFLELEPLTYIRGRSLPDQFFIIDEAQNLSPHEMKTIITRAGENTKVV
                     LTGDPYQIDIPYLDSQSNGLSVSVEKFKGEILVGHITLDKGERSALADLAAKYL"
     gene            9113..11002
                     /gene="alr"
                     /locus_tag="PLW08_03995"
     CDS             9113..11002
                     /gene="alr"
                     /locus_tag="PLW08_03995"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPL51796.1"
                     /translation="MLRRKIFERSWVEIDLSAFRTNLNILKSFLEPQQQFMMIVKADA
                     YGHGAREISKIACEEGAVYLGVANPEEGRLLRLQGCKEPVLILSPCLSNEINTILDND
                     LAVNVSSLDFARALNKTAKTKNKQAVVHLKVDTGMHRSGILDSEFIDFFNKIAKMDNL
                     LIEGIFTHFASAENDPDFSKQQEERFWNIIDNLPFTPTYLHINNSNALVNGLGKHSNL
                     VRLGIMAYGIQVPGNKDIALQPVMTFKTILSQIKHIAKGESVGYNRSWTAKKDCFYGI
                     LPIGYADGYDFMLSNCGVVFLDNKLCNVIGRISMDMICVDLSPIENPAIGDVAILLGG
                     SALETRAENLVSKYGGNSYELLCQVGRRAKRYYFQNGKMIYSTPLSRRDFVPDDFSDS
                     KLNQIIESAIAQRLQSIEIGELIYREMLRDFFYYKDKDIHYRHNFQHQVIFSHSLNAG
                     YFNATTTLNFDKVLSNDYFMVACASSDEVLRRYFKRSDVEYRWLMDDSFELDSKNFIV
                     CLAQVDDLILQTTLHYINGCLEIRCSHPELKKKIGKRVHFTINTLTLYPCSSHQFSVF
                     ITELTRGVDISFQYPLTINTVDCVPFFSGQDKDPIIQRNNGEIRVSSKPEEWIFPISG
                     VVFAY"
     gene            11100..12053
                     /locus_tag="PLW08_04000"
     CDS             11100..12053
                     /locus_tag="PLW08_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51797.1"
                     /translation="MKRLHLIYITMILCVPLIAGSFNWYSQNDERWKSEQLGSGKRSI
                     GSSGCVLSCLSMLLNAEASNEYMTPDRLNEWLKRNGGYSGSNMRWQIPGEIDGSGLGL
                     ELVAQSTVRNDWDFLSNELEKGNKVIVKVAGRRSHWVLVIKQNGPKNKPSSYIINDPG
                     MKEYEERTLAYFGGFKSARSYSGNWLDEQAFDLDSEINVVPVSQDELFLYDVAKLPHP
                     ADVYVTLHNNLPVEISGYFILGLFDEEGNYLRTVDYEYATVEASGNYDLLYELPDVKP
                     LNEEHQDLKIIYSKYFSNMPSLTESIELINKGIRNYTNTTN"
     gene            12634..14670
                     /locus_tag="PLW08_04005"
     CDS             12634..14670
                     /locus_tag="PLW08_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPL51798.1"
                     /translation="MNNYFMETHHHEWILTNRRGGYALGTGNLINQRKYHGLLVSSDS
                     KFNRFHLVAGIEEKVEWRGEFFHLDSNNYSNCIYPEGFLHLVKPWLRPYPIFLYSALP
                     HQNDILILKEIMMDESTNTTLVKYTNLGHHLLHFELHPKFTMIPHHELNKPGTLDFLN
                     FITEIETPDKFTRFSVQRQDNNIKLYGALLSGEVIPNRYVYYNVFYPWEVMSGYEGIG
                     DQITLFQLGFDLRVGQSNYLLFSDTAIEDAKTLIERIENRYADLPKPLDYPYASDKND
                     TLLNNLDYNDNIIFPYDNYLKILEFALKDFLANDDIVAGFPFYGAWGRDTMVVVNALL
                     HCPNQLETVEKILNKYRGYIKNGLIPNMIAESGRETNYDSVDSTLWYIILLWKLGKRK
                     QTVKYWKDVIALTEEIINSFLHNESHPFSVRYDGLIELHPDFAHGTWMDVRIDGKAIT
                     PRNGAPVEINALWYNALCSYEAMCLAYCQKSRKKYQAKEEFIQLKELVKQSLQKFYQD
                     GYLADRIVGEELIMEIRPNAIIALALPWQAFSQEILQQVLERSFKELYTNYGIRTLSP
                     KDIRFHKKYYGTQKERDLAYHNGSVWAWLLGPFCGLYERAYKGIKTKEEIIEALTSFI
                     STFRNSFMKGHIASIAEIWDGDAPHFPKGAPAQAWSVAALYNIENYILFLQEGK"
     gene            14667..16010
                     /locus_tag="PLW08_04010"
     CDS             14667..16010
                     /locus_tag="PLW08_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51799.1"
                     /translation="MKILMFTWEFPPLISGGLGMACYGMVKALLSQGIKIDLVLPTKE
                     LVYFPLREESDADTLPAVFIEPEKHKEYTKRTFSTLHERLEYIGASTHPESYFNLSEI
                     KDYVSSIKKTFWLTETVTTEEHNIWKEMTAHLIGEEDLIRKVQEYTLRASRFAEILDY
                     DLIHAHDWLTYPSGILAKQISRKPLIVHIHATEFDRAGGPGDERIHKIEHLGMNYADK
                     VIAVSQYTAQMIMSRYRIDTGKIRIIYNAFTVSETTLHNKKRIFKGPTILYLGRITLQ
                     KGPDYYLDMADKVLKVHPEARFILAGTGDMQRQILRRSAALRLKNRFLFTGFLNRKQV
                     ERILQAADIYVLPSVSEPFGISPLEAMAYGITAIISKQSGVAEVVHNAFKIDYWDIDL
                     WAETINHLIENPEECAKIGMEGRKEVQKIQWNEAADKIRQLYAETLSEYSKRMEL"
     gene            16021..17220
                     /locus_tag="PLW08_04015"
     CDS             16021..17220
                     /locus_tag="PLW08_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPL51800.1"
                     /translation="MLNIVFYFQVHQPYRLNHFNVLDIAHNAKIFNDKLNGDIMRKVA
                     NKCYLPTNQLLLELIEKYEGRFKVAFSISGTALEQFKLYSPETLDSFKRLVDTGCVEL
                     LGETYYHSLAFLFDTNEFLDQVYLHRDLMQTEFGYYTTTFRNTELIYQDRLSDIIFEI
                     EGFKTILTEGVDRILQWRSPLYAYKNYSKNMNLLLKYYQLADDIAFRFSNRDWPEYPL
                     TVDKFVHWIDQLTLAEKGGKNLFLNLFMDYETFGEHQWASTGIFDFMRHFPDAVLERE
                     HLGFANPKDVSHLANYQQESLSFPEPVSWADEQRDISAWLGNEMQQNASETFYELLNR
                     IKEKGDEELLRTARMLSTSDHFYYMCSKYFQDGDVHKYFSPYDSPDQAYIYYINALAE
                     LEELLLR"
     gene            17227..18642
                     /locus_tag="PLW08_04020"
     CDS             17227..18642
                     /locus_tag="PLW08_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL51801.1"
                     /translation="MKGKILILEDDIVLSEQIANVVKHYNYEVMQTVNSDQFFEVLRQ
                     FQPDVILLDVFLVGSKLNGIQVLQHLKNDMDLNYKVIVISGEVTSSQINEIRTLGAYH
                     FIEKGSAFSINQLLLHIENAITLKRQEEEHIGLQIEYINLKKQFTRSFPFIGESNAIK
                     KVREQIIRLASVDEDIFLIGETGTGKEVAANYYYIYSPRFGKPFHTVNCSALSETLIE
                     SELFGHIKGSFTSADRDKTGFFEECTNGILFLDEVTNLSLMSQSKILRAIENKEIQVV
                     GGNLKKVDTRLIFASNAGLEKLSDENIFRKDLFYRIEGNIVELPPLRERDDDILLLIC
                     YFLTSFSSQYRVIDRLDLPALKDDLLNYSWPGNVRELRNFCKFIMINEQKINNNVIKK
                     HLQQKINQFRFSKNYSSEKYTKIHNLKDSVASFERDYLLYYLSLNNWRISQTAEAIGL
                     ERTTLYKKIKSLGINHLKMED"
     gene            18645..19520
                     /locus_tag="PLW08_04025"
     CDS             18645..19520
                     /locus_tag="PLW08_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPL51802.1"
                     /translation="MLQENLGLKILSLLIAVFVWLQSLLISEQKSVVNLPVALSSIPK
                     NITLDNVPNHIPFNVRGRGLEIIKLKFSRTKVLLDASKIKPGSDILSVSDYTIDLQEN
                     IQVEILGPVEQREISVYADVFHQKKVPVELSFADSYTRKHFTALNYTIIPEQITISGP
                     KSKVQQIDRVTTEPVSREMLEQREVTLKINPLGSEVSASEKQVKIYLSTSYITTKIYH
                     NLPLTVPPGKSSIPSSVTVKLSGNSDILQNLKPTELKVSIASQPDEQGFYPVLVELPD
                     TVKLIAVTPQKVRLK"
     gene            19521..20537
                     /gene="tsaD"
                     /locus_tag="PLW08_04030"
     CDS             19521..20537
                     /gene="tsaD"
                     /locus_tag="PLW08_04030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPL51803.1"
                     /translation="MPVQYILAFESSCDDTSVAIVDTDYNVIVNLISSQPEHLEFGGI
                     LPELASRLHLKNIVTLTKVALNASKLNLQDISAIAVSINPGLIGSLIVGLAFAKGLAW
                     SLSLPLITVNHILSHIFANFIEHKAVEPPFLALVVSGGHTELVHFDTLTTFTVVGKTL
                     DDAAGESFDKAAKLLGLGFPGGPAIDELAQKGNPNFIKFPRALPQKNNFNFSYSGLKT
                     AIRTWLVNQNPETLQDKLPDIAASVQQAIIEPLVHKTVLWARQHKIPYILLAGGVAAN
                     SALRQQLTATSAKYGIKVFYPSNALCMDNAAMVGAAAIPKFLAKNYAPLSINVSSLKG
                     TRQV"
     gene            complement(20744..20819)
                     /locus_tag="PLW08_04035"
     tRNA            complement(20744..20819)
                     /locus_tag="PLW08_04035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20784..20786),aa:Thr,seq:cgt)
     gene            complement(20815..21483)
                     /locus_tag="PLW08_04040"
     CDS             complement(20815..21483)
                     /locus_tag="PLW08_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51804.1"
                     /translation="MRKFLLTLVLLVLAFCLMAEEADTPKINFALLNPKEINLELRVY
                     APMQRVYITADFSVEKDSLPDAVYHSLFLTKDARIEYFLINDKFVPPVYSTKLVPEHF
                     NPVFPYPEMLNEDSNLNCFSFYLPNYEGKIKFTLRYSIPIPEWVKESDIRGYVTFSSA
                     QNWYPRNLTGPCKVYARLISSTYYTMELGTQCTIKEKDDIRITEGYFIDSPAEQSIFK
                     IIKS"
     gene            complement(21559..24507)
                     /locus_tag="PLW08_04045"
     CDS             complement(21559..24507)
                     /locus_tag="PLW08_04045"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPL51805.1"
                     /translation="MLQKFLMSIVILMCVYSSLSALRYTTLEDFESGVVSLNSWSIEE
                     DLQPDAWSLDTSTPDSSSYCLKLTGNCYKLQQITPYAIDSTGVIQVQVKTSSNPRIQG
                     IGFTDGVHNILYSFAGSRILDIEVWIPVYQGAFSNGVWNTFQLPLALDWQAFWGYLPV
                     INGIIYINDLDGISSRSVWFDNIIDISSDLPVPPTVSISTSYPVKNTAVYFYSNVIDP
                     DSDTFTYEWSFGDSTFSNLPNPYHLYTIASAYPYTVTLKVTDDTGKVGFASTEVILET
                     GASHLPVSLNFVGDIMLARRYENPGGIIPTLGVNAIFSPTKPYFGEAADISVANLEVV
                     LANVGVHHPTKSVYYRGNPANVSGLVYAGIDVVSTANNHTMDYGIEAYQQMQGLLSNS
                     GIKYSGCGANSYEAYLPTFYHCRGLNIAFLRSSDRTGQYNNAQPFLQAGYNKPGFAYM
                     TPYYVEQQLQAVEGIADLKIVEMHGGSEYSIAPGAGYDKEFNPFLEDTEDEDYFYRND
                     VPHQWDIEIRHSAIESGADLVIVHHPHIIQGLELYQNKLIAHSLGNFVFDLDYPETMP
                     TMILYADAYFDGFRNFRIKPFYIDQYIPKPATGQLAVYILDYIASRSMDFNTRVMVDK
                     ENLEARVLMEDENPPVTTNAFSSWQHLLFHSGGMSYTSPIKLPRRGSISNIINVEPPA
                     EYQFRLGQEHIWFGNFENEGSTLWIPPAYSTTDFIDGERSAKLTTTGAGSVTSTISQR
                     CKIYDNTKKYTLHGWLKTENVSLANISIRFYTSRTAAVASYTDYVTENIIGTNDWTYY
                     YKELNLPTDVFYYEIRLQMTGSTGMTAIALFDNVGLIEWTEWTATADLINIPWPNNYY
                     WIQARTADTPKSMSFSLVERSFGVQRANTSNHFSKMKPELNISPNPFNPETIIYCVLP
                     VSGKTTIKIYNIKGQLVKELYKGELEAGIHRFNWNAKDIDNRSVGSGVYFIYLENGNS
                     KVIRKAVLMK"
BASE COUNT         7757 a         4786 c         4500 g         7702 t
ORIGIN      
        1 aaagtatatc catccaaact ataattaccg gaactgagag tagtgtttgg ggtctgtttc
       61 atataaatag aaacactttc aatcgggttt atttctacac cttgtgcttt ataataggca
      121 agggatttta ttgctcccgc aacaccaaat tcactggcaa gataaatagc ttcgtgagca
      181 gaataattat accatctatc aaaaggataa gtttgcgttc cagttccagt tcctattgta
      241 atttctaaag gtgctccaat ttccagaatt atattcgttg aagcagaata agttccggca
      301 gctgcagaac aaaataaatt agctaaagtt cctaccgcaa ctgaggcatt tgcactaaga
      361 ttaaaaacaa gtgttgtatt tccattcccg gatattggag caaactgagc agttcctgta
      421 ataatattaa ttccttcggt agaagtaaaa attgttgccg ttccaaccgg gctggctact
      481 tctcctgtat tcagtaattg aacagagagg tttacagttt cgccggggtc taaaattcca
      541 tcattgtctc cgttagaatc taacacagta tagtttccga attccagaat tggaacatag
      601 acatctaaag aaaaggaata gttccaattt aatccactac cggaaagagc tagagtaaaa
      661 ttaattctat gtcctccagg gcaggatgga gaaacactta aattaaaatt actttgtggt
      721 aagccctgtg cctctccggc aatatttcca taattagcag tagcattatt aattgtgaca
      781 taagaatcgg aacaactcaa cacaccagta acattctcag cagtagaaag tccattgttt
      841 ctaagcagga tttgcagttg agctgtttca cccggttcca gatgattatt actattaggg
      901 tcaataacag ttgtttgagc tatagtaaga tgaggtgtac cggatataat ttccggtgtg
      961 ctgataagca aagctttttc gttactcaag ggactcgcag ctgtaggata agtattgttg
     1021 aaagtatatt ccaatccatc agttccgtaa tggtctttaa tacctatagt gcagtaattt
     1081 ccatgcggat atacatctcc actaccctgg tctatattat taaaaacctt atattgtaat
     1141 tttatttgac catcattggt aatagttgga taataaacag ggtcatataa tatcgcttga
     1201 aaggtctcca aactggaacc gttatagcca gaaattatat tataccattc cactacataa
     1261 taatgttgag ctgaattata atatgtataa actccacttc cagttcccag ttgaaggtca
     1321 tcccaaaaga cagcaatcat cggattggga cctcctgctc ctggtaaacg ccaatttctc
     1381 cagtccgcat tttgggtcgc accaaaagca ataaaaccat tggaacaaat ggatgcctga
     1441 gtatattgtc taccataaaa ggtaaaggta aagggtaaat taaccgtctg aatagctata
     1501 gcacttgtat cgtcaccttc atctgatgta tttcccgggt ctgttaaaga tagtaaggtt
     1561 ccacttcctc cttcagcagg agcaatagga atccattgat aacttgggca caaaggatat
     1621 gccgtatccg tttgatcata aataaaatag ccataagcat cctgacccaa aggatcgtga
     1681 acagtcgttg tgccaatcgt aatacttaaa tgtaaggtct gggtatatcc attagcatta
     1741 gaaagtgtta gaatcaacgg aatcaccatt ccatttatac tgctatccca ggcaaaaaca
     1801 gtaaagctgt tagaactatt agtaactgta gaattaggag caatattgcc aaaataaccc
     1861 tcagcatctt gaacaacaaa atagttactg agcgactgca aattgccgta aacattatgt
     1921 aaagtagaaa taccggtgtt ctttagggaa aaagtaaagg gactggaaag tccgggagtg
     1981 ataatattgc ccgttatacc cacaaaagta taactttgaa tttccaggtt gcctcctctt
     2041 acggtcagag ggatatttat cgtccaattg tttccggcac cttcagccgt aagcactaaa
     2101 gtaataatat gttgatcagg acaggcaggg gaaacggaaa aaacaatggg agtaatgttt
     2161 tctcccaaac caccagcaac aatatcacca aattccaggc ggtcatattc tattaaagta
     2221 acataagaat ctgtagtaga taccctacca ttaataaaga gagtagaaga acttgtattt
     2281 ttcagtgtta catagaggtt aacttcttct cctgcattca gtgttccatc tccattgccg
     2341 gtagttgtat catcatccag aacaaaactg tcatagacaa ttcccccacc ggtatttaaa
     2401 gttatggagc tcatataagc gatataatca tctttagcaa cagttaaaac caagttttgg
     2461 gaaagtcctg ctggtaaatc aatattaaca aatcccgaag catctgtaat tccggttaat
     2521 tgtaaactgg aagtagtagt taaagttaca aaagcacctt caacgctttc tccattattg
     2581 tttgttgctc caattctgat attggcagaa ccggaaggaa tggttgacgg agctgaaaca
     2641 ataaaactat tgggagttcc tacataaacc atagcagtag gatcacccat taaattacag
     2701 taagcagcaa aatcctgtgc ctgagtggga tgactaactc cataaactga atgtaaatan
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agcaaaatcc tgtgcctgag
     2881 tgggatgact aactccataa actgaatgta aatataactt tgcatatagc attgcggaag
     2941 acatctcccg cattccgaga ttgtaaattc cgtgaaagat accaacatcc aaacaattgt
     3001 tcattggagt atgagtgctg gaagtagcca ttccaattgc agttattgca cctcctaaag
     3061 aagcagcggt tccaaagcga ataacttgtt ccgtagtaga tggttgagat gaaccgccaa
     3121 aacttccagt gctgcaagta ataaagaccg catgaaagag tttgtaagaa ttatataagg
     3181 aagacattgt acttggccaa ctgttcatat ttatccaacc acgaaaatta taacacatta
     3241 caccttgatt aatagcatta ttgatcgttg tgttactggg gctggaacca tagacctcag
     3301 tataagtata agcaggatta atttcttccg agatatcacg aatgtatcta ttagtataaa
     3361 tcgttgaaat acctgaatca ccggtatccc caactaaaac cattctattt aaccaatcgg
     3421 tgggatgttg tccaatgttc ttttccagag agatgatttt tgctaagata acatccaaat
     3481 cagcagtaga agttacagaa attctaccaa tcattacatc acccaggtta tcacttcctg
     3541 ccagccaggt atattgatag tcaacataat tgttattact tggaacagca atagtgccat
     3601 ctgtatcacc aattaagaca atatagtccg gacgcgttgc aggattgttg taagctgttt
     3661 gaatataact cttaatattg gtattggttg ttccggtaac accagtgcta actgcgttta
     3721 caataaagcc cctttgcttt ttccaggtaa cgaattcatt tattttagat aagtaggttg
     3781 catctgtata attgccatag ataaccagca tcacaggatt ccgatagtca atttcccgtg
     3841 tttgccgcag ggattcatag tttaaaatca gccctctata gatactgtca aaactacaag
     3901 aaataaaaga actggaattc aattcattga tagaaggtct gctgttaaat gttaacttta
     3961 tatccagatt ttcactaaca agcagtttcc gatttatccg gtcatattga aagggatata
     4021 tattaagatt tacaaccctg aaatcacgca atatctgggg ctctgagata acaacattag
     4081 cagcaggata atgaatatcg gtaaatcttc cctgttggtt ttccctattc agagcagcat
     4141 caaaatcggc agtaaattca ttaatagaag attttgagga attagaaaca agcaaatcta
     4201 caccccccct attcggaata gcaatcattg tggaaaatat cggcaatgtt tcttcttcat
     4261 ctataaaaag ataaggtgtt tcctgaactt taactttctt tcttatttcg tttttaacat
     4321 taatttgttc aatttcccat tgcggtaaag taaattgaat tcttatttcg ctatcattta
     4381 tttccttaat cttaaaagga ttgttttcgc cgtttgtatt gccggatagc gctattggca
     4441 ataacaaaat agttataatg agcagcagga aaaattggga tgacttcatt aagaacctct
     4501 atattcggtt tttttcatta tataaagcaa ttctaatgcc aaattttaag cttattgcag
     4561 gataacccga tattccctca gtaacttatt gttatattga atgtaagctt ttcttcttta
     4621 agtgcattac tcaagcattg ctcacccatt gctaatgcca tccgtaatgg gtaagcaatg
     4681 ggtaagcaat gctactaagg aagataaatc gtaaaaaagg atgctgagct acggtaagct
     4741 caagctacac aattctggtt ccggttttgc cttcaaaggc atccacaatt ttttcaatgg
     4801 aagtaatcaa tacttccgaa ccaccgcgtt ccagaaaatc aatagctgcc tgaattttag
     4861 gacccatact tcccgcggga aactgttttt cagcaaaata gcgtttggct tcggcaactg
     4921 tcattctatc caagtcctgc tgattttctt taccgtaatt aatagcaacc ttttccacac
     4981 ctgtcaaaat cacaaagagg tctgctttaa tttccaaagc taaaagtgag gaagcaaagt
     5041 ccttatcaat aaccgcatca acaccttcgt aggtgccatc ttcttctata taaataggaa
     5101 ttccgccacc accaactgct attactatct ctcccctatc cacaagttct ttgatggttg
     5161 cagcgggaat aatttgaatt ggataaggtg aaggaacaac acgccgaaaa ccttttccgg
     5221 aatcctcttt cagtatccaa cccaatttct gatgtaattc ttctgcctgc tgagctgtat
     5281 aataggtact tccaacaaat tttgtcggat tcagcatact gggatcattt ttatctacaa
     5341 ctacctgcga aataatagtt ataacccgtt ttttaatacc gcttttatgt agtttatttt
     5401 gcaggctttg ttctatcata tatcccatag ttccttccgt agcagcgttt aatacaccta
     5461 aaggcaattg gggaatgccc tctttttcac cggcttcctg ttggcgtaat aaatttccta
     5521 cctggggtcc gttgccatga gttatgctta aatgatagcc ccgtttgatt agttctacga
     5581 ttccgctaag ggattcacga gtattggcaa attgctgagt tatggttcct ttttcacctg
     5641 ctttgataat agcgtttcca ccaagggcaa gaacagctgt tttgttcatt ttccttttcc
     5701 tttaactaat gtaaattttg atgaagcaga aaagttaatt ccttttaatg taaaaagtga
     5761 ataaacttta ctgccagagg atcacaaatg gaaaaaggag gcgacaaagc acctcctttt
     5821 atagaaacta ttatttttta gaggatttct taccgaaatt cttcagaaca tccgctattg
     5881 taggaactcc aatttcctgc aattcgtaac gcacttgaca agcgggctta ttgattttaa
     5941 ttaaacgacg caggtctatt ggagtggcaa taatcacaga atcacattca gcactattga
     6001 ttgttttttc caaatcctta atctgcttgg aactataacc cattgccgga agcaaagttc
     6061 cgatttcagg atatttttcg taggtctgct taatttcacc tgcagcccag ggacgcggat
     6121 caacaaggtc agcacaacca tatttttctg cagctacaat tcccgctcca taagtcattt
     6181 ctccatgggt taaagtagga ccgtcttcca caaccagaac ttttttaccg taaatcattt
     6241 ccggatggtc tacaaaaagc ggtgatgcag cttcaataat ttgagcttta ggattgataa
     6301 aacgaacatt ttcgcgaacc tcattgatat catcaaaatc ggcactatca attttattga
     6361 taacgattac atcagccaaa aagacatttg tttcacccgg atagtaagaa atttcatctc
     6421 cgggacgatg aggatcaacc acaactatct taatttcttt atcagaagta taaaatggaa
     6481 tatcattatt acctccatcc caaacaatta catccgcttc tttttccgct tcttttaaaa
     6541 ttgcctcata atccactccg gcataaataa cactattcat cattatatgc ggctcgtatt
     6601 cttccatttc ttcaattgtg cagttatatt ttttcaggtc ttccagtttg gcaaaacgct
     6661 gaactttttg tttaacaaga tcgccataag gcataggatg acgaatggaa acaactttca
     6721 gcccttttgc cttgagagct ttcacaaccg ctctggtcgt ttgtgattta ccgcaaccgg
     6781 ttctaactgc acaaacagtt acaaggggtt tactggtttt aatagtagtg ttatcttttc
     6841 ccatcaacat aaaatcggca ccacaagcat ttactaagga tgctttgtgc ataactacct
     6901 caaaaggaag gtcactgtaa gctaagacca ctaaatcaac tttgtgtttc ttaatcaggt
     6961 ttttcagttc actttccgga tagatattga ttccttcagg ataaagcttt cctgctaaag
     7021 cggaaggata ctttttgtca tcaattccag gaatctgtgt agcagtaaaa gctacaactt
     7081 catagtcctt gttatcgcgg aaaaaaacat tgaaattgtg aaagtccctt cccgctgcac
     7141 ccataatgat aactttgcgt ttcattaatg aatctccttt aatttattat agttacatat
     7201 ttgttttcac cgttttaatt agcgttttac cgtcaaggaa aatctggaat ggcagagaga
     7261 gcatttcaac tctctcaacc tttttaaatt ccttcttctt ttttctcttt tcctcttttc
     7321 ctctttgtaa aattatctct ttttcaattt acaaaataaa atccgttttc tgttctttta
     7381 tctgtaaaat cggtgtaaga tttgcgtaat ctgcagaaaa aatacctgta ggaaaaacct
     7441 tgacaaaatc cttctttctt aagaaaagga ttttagtata aaatgctgaa gtggtgaaat
     7501 tggtagacac gctagtttca gggactagtg agcaatgagc tcatgggggt tcaaatcccc
     7561 ccttcagcac cattttttta ttaactacag gtttttttta cttctatcaa tccttttttg
     7621 caatcattat caggaaaata atcattgact atccctgccc tattgtaatt attgttttat
     7681 tgttcttgat tcaagataca aaccccgtta gaagggtaac agacccgaaa ctgcgtgata
     7741 aatataaaaa acaaaggaag ttatacaaat ggaaaggaaa atttttattc tggatacaaa
     7801 tgtcctgatt cacaatcccc aagcactttt ttcttttgag gataatagag tggtcttgcc
     7861 tattgtagta atagaagaaa ttgaccagtt taaaaaaggt gtggatgaga aaagccgtaa
     7921 cgcgcgtcaa gttggacgct atcttgatgc tttacgcaaa aaaggaaaat tacaggaagg
     7981 tgtgcccaca gaaaatggag gcacaattca agtaacagtg aataaagaaa taaccaacac
     8041 cgcttcggag ctgttgtttc ttgaccgaaa tgacaattta ataataagta ctgctttgta
     8101 tttcaaggaa aaatatcctc aatccattgt aactctggtt tccaaagatg taaatgtacg
     8161 tgttaaggca gattgtgtag gcataaatgc agaaaatttt gaaaccgata ccattaaata
     8221 cgaggaattt tttaccggtt gggaattcct tgatttggaa ccggaacaat acagagaact
     8281 ggaagaaaaa ggatatataa acaataactt cggtgatttt ttccccaatc agtttgtgcg
     8341 tgtttctgtt cctgataagc ccgatcaata taagacctta cgttatcatt atggacgtaa
     8401 tcaactctat gtaatcaatc attatacggg acaacaggtt tttggtatta aagcccgcaa
     8461 ttttgaacag gaaatggctc tggatattct tttggatgac agtataaagt tagtaagctt
     8521 atccggcaaa gcaggaacag gaaaaacact tttagctatg gctgccggct tacaaaaggt
     8581 ggtggaagaa aaaaaatatt cacgccttgt agtttcccgt ccaatttctc ctttgggaaa
     8641 agacctgggc tatttacccg gcactaaaac cgaaaaattc aatccctgga tgcagcccat
     8701 ttatgataat atggatattt tgctctcttc gcatacggat aaaagccagg aaggttctcc
     8761 aagcagaaaa aaaaccgata ttaatgattt aatggattac ggttttctgg aactggaacc
     8821 gctaacttat atccgaggac gcagtttgcc agatcaattc tttattattg atgaagccca
     8881 aaatttgagt ccgcatgaaa tgaaaaccat tattactcgt gcaggagaaa acaccaaagt
     8941 tgttcttaca ggagacccct atcaaataga tattccctat ttggattccc aaagtaacgg
     9001 actttccgtt tcggtagaaa aattcaaagg cgaaattctg gtagggcata taactttaga
     9061 taaaggtgaa cgcagtgctc tggcagattt ggctgccaag tatttataaa ctatgttacg
     9121 aagaaagatt tttgagcgta gttgggtgga aattgacctt tccgctttca gaaccaatct
     9181 gaatatcctg aaaagctttc tggaaccgca acaacaattt atgatgattg tgaaagcgga
     9241 tgcttatggt catggagcca gggaaataag taaaattgcc tgtgaggaag gagctgtcta
     9301 tttaggagtt gccaatcctg aagagggtcg tttattacga ctgcaaggtt gcaaggaacc
     9361 tgttttaatt ctttctcctt gtttaagtaa cgaaattaat accattttgg ataatgacct
     9421 ggctgttaat gtttcttctc tggattttgc cagagcgtta aataaaaccg ctaagacaaa
     9481 aaacaaacaa gcagttgttc atctgaaagt tgataccggt atgcatcgca gtggaatttt
     9541 agatagcgaa tttatagatt tttttaacaa aatagctaaa atggacaacc tgctgattga
     9601 aggtatattt actcattttg cctctgcgga aaacgatccg gatttcagta aacaacagga
     9661 agaacgattc tggaacataa tagataacct gccttttact cccacatatc tccatattaa
     9721 taattccaat gccctggtaa acggtttggg aaaacacagt aatttagtcc gtttgggaat
     9781 tatggcttat ggaattcaag ttcccgggaa taaagacatc gctttgcaac ctgttatgac
     9841 attcaaaaca atactttcgc aaataaaaca tattgctaaa ggtgaatcgg tgggatacaa
     9901 tcgttcctgg acagcaaaaa aggactgttt ttatggaatt ctaccaatcg gttatgctga
     9961 tggctacgat tttatgcttt ccaattgtgg cgttgttttt ctggataata agctctgcaa
    10021 tgttataggt cgcatctcaa tggatatgat ttgtgtggat ttgagcccga tagaaaatcc
    10081 tgccattggc gatgtagcta ttcttttagg aggttcggct ttagaaacaa gagcagaaaa
    10141 tctggtatcc aagtatggag gtaattctta tgaactgctc tgtcaggtag gacgtagagc
    10201 taaaagatac tattttcaaa acgggaaaat gatttatagc actcctcttt cccggcgtga
    10261 tttcgttcct gatgattttt cggatagcaa attgaatcaa ataattgaat cagcaattgc
    10321 tcaacgctta cagagcattg aaataggaga gctcatttat cgcgaaatgc tgagggattt
    10381 cttttactat aaggataaag atattcacta caggcataac ttccaacatc aggttatttt
    10441 ttcccattct ttaaatgccg gttattttaa cgctacaact accctcaatt ttgataaagt
    10501 gcttagtaac gactatttta tggttgcttg tgcttcttcc gatgaagttc tgcgtcgcta
    10561 ttttaaaagg agcgatgtag aataccgttg gctgatggac gatagctttg aactggatag
    10621 taaaaacttt atcgtttgct tggcacaagt agatgatctt attttgcaaa ccactttgca
    10681 ttatataaat ggctgtttgg aaattagatg ttcccatccc gaattgaaga aaaaaatagg
    10741 aaagagagtt cattttacca tcaatactct tactttatat ccttgttctt ctcatcaatt
    10801 tagtgttttt atcacggaac ttactcgcgg ggtggatatt tccttccagt atccactgac
    10861 tataaatact gtggactgtg tacctttttt ctccggtcag gataaagacc ctatcattca
    10921 aagaaacaac ggagaaataa gagtttccag taaaccagag gaatggatat ttcctattag
    10981 tggagttgtt tttgcttatt gaaaacaaaa aaatgtttga catatttccc tctccagaat
    11041 agtggtttct taaagttaat ttctatattg ttgtagttgt tagctaaggt gacttgctta
    11101 tgaagagatt gcatttaata tatatcacaa tgattttgtg tgtgccactg attgcaggta
    11161 gctttaactg gtattcacag aatgatgaac gatggaaatc agaacaattg ggttcaggaa
    11221 aaagatcaat aggtagcagc ggctgtgtac taagctgcct ttctatgctt ttaaacgccg
    11281 aagcaagtaa tgaatatatg actcctgacc gcttaaatga atggttgaaa agaaatggcg
    11341 gctattccgg aagtaatatg cgttggcaaa ttcccggaga aattgacggc agcggattag
    11401 gactggagct tgttgcccaa agcactgtta gaaatgattg ggattttctt tccaatgaat
    11461 tggagaaagg aaacaaagtt atcgtaaaag ttgccggcag aagaagtcat tgggttctgg
    11521 taattaaaca gaacggtcct aaaaataagc cctcttccta tattatcaat gaccccggaa
    11581 tgaaagaata cgaagaaaga accctcgcct attttggagg gtttaagtcc gctcgttcct
    11641 attccggaaa ctggttggat gaacaagcat ttgatttgga tagtgaaatt aatgtagttc
    11701 ctgtttccca ggatgaactt tttctttacg atgttgccaa attgcctcat cctgccgatg
    11761 tttatgttac tttgcataac aatttaccag tagaaatttc cggctatttt attctgggtc
    11821 tctttgatga agaaggaaac tatctgcgca ctgtggacta tgaatatgca accgttgaag
    11881 ccagtggaaa ttatgattta ctctatgaac ttccggatgt taaacctttg aatgaagaac
    11941 atcaagacct gaagattatt tatagtaaat acttctcaaa tatgccttcc ctaactgaat
    12001 ccattgaact catcaataaa ggtattagaa actataccaa tactaccaat taaaccaaat
    12061 aacacaatag caataatggt tattatcatt tccctcattt taaccatatt gtagtaaaaa
    12121 ttccgtagaa acgacagata ctaacaacgg gttttaaccc ggcgaaaaaa tatagctact
    12181 aaagagaaaa aattcctttt atcataccaa atcaaagttt tttatcaaat agttataatg
    12241 tttccgctaa ggtgtttgca tcttctcctt attttagttt tttatttaat cattcccatt
    12301 ataaacattt tataatatcc atagattttt gtcaagattt gacagattat tattactttc
    12361 ctttcacgaa gagcttgttc aaacatctat ctcaaccctc ttaaccctct caaccctctc
    12421 aaccttctca acctctttta aattcattct tcattccttc tctttgtaaa aaaatcctaa
    12481 aaatcccttt catcctgttc atctcctacc aattatcaat caaaatctgc aaaatcagtg
    12541 gaatctgtga gaggttataa aaaaggactt gacgaaataa ccaatatgga aaaaaaatca
    12601 acacaactta ggattagaat ttgaggaggt ttgatgaaca actattttat ggagacccat
    12661 catcatgaat ggattctaac taaccgtaga ggcggttatg ctttaggaac aggaaatttg
    12721 atcaaccagc gaaaatatca cggtttactg gttagcagcg acagtaaatt caatcgcttt
    12781 catttggtgg ctggcattga agaaaaagtt gaatggcgag gagaattttt tcacctggac
    12841 agcaataatt acagcaattg tatatatcct gagggttttc tgcatttagt aaaaccttgg
    12901 ttgcgtccct atccgatatt tttgtattca gctcttcccc atcagaacga tattttgatt
    12961 ctgaaagaga taatgatgga tgaatccacc aatacaactt tggtgaaata taccaatttg
    13021 ggtcatcatc ttctgcattt tgagcttcat cccaagttta cgatgattcc tcatcatgag
    13081 ctcaataaac ctggcacttt agatttcttg aattttatta cggaaattga aactccggat
    13141 aaattcactc gttttagtgt tcaaaggcag gataataata taaaacttta tggtgctttg
    13201 ctttcaggtg aagttattcc caatcgctat gtttattata atgtttttta tccctgggaa
    13261 gtgatgagtg gttatgaagg tattggtgat cagataactc tttttcagct tggttttgat
    13321 ttgagggtgg gacaaagcaa ctatcttctt ttcagtgata cggcaattga agatgccaaa
    13381 actctcattg aaaggattga aaatcgttat gcagaccttc ccaaaccttt ggattatcct
    13441 tatgcttccg ataaaaacga tacccttttg aataacttgg attataacga taatattatt
    13501 ttcccgtatg acaattatct taaaatcctg gaatttgccc tgaaggattt tttagccaat
    13561 gacgatattg tagccggctt tcctttttac ggtgcttggg gaagagatac aatggttgtg
    13621 gtaaatgcgc tcttacattg tcccaatcaa ctggaaacgg tggaaaaaat cctcaataaa
    13681 tatcgcggct acattaaaaa tggtctgatt cctaatatga ttgcagaatc cggcagggaa
    13741 acaaattatg atagtgtaga ttctacttta tggtatatta tcctgctgtg gaaattgggt
    13801 aaacgcaagc aaacggttaa atactggaag gatgttattg ccttaacgga agaaatcata
    13861 aacagctttt tgcataatga atcccatccc tttagtgtgc gttacgatgg tttaattgag
    13921 ttgcatcccg attttgctca tggcacatgg atggatgtgc ggatagatgg taaagcaata
    13981 acgcctcgta atggagctcc agtagaaatt aatgccctct ggtataacgc tttatgttct
    14041 tatgaagcta tgtgccttgc ctattgtcag aaatcccgaa aaaaatatca agcaaaagaa
    14101 gaattcatcc agttgaaaga gctggtaaaa caatccctgc aaaagttcta tcaagatggc
    14161 tatctggcag atagaattgt aggtgaagag cttataatgg aaattcgtcc taatgcaatt
    14221 attgctcttg ccttgccctg gcaagcattt tctcaggaaa ttttgcaaca ggttctggaa
    14281 cgcagtttta aagagctcta cacaaactat ggaatccgca ctttgagtcc taaagatatc
    14341 cgttttcata agaaatatta tggaacacag aaagaaagag accttgctta tcataatggc
    14401 agtgtttggg cttggctgtt aggtcctttt tgcggtttgt atgaaagagc ttataaaggt
    14461 ataaagacca aagaagaaat aattgaagcc ctcacttcct ttatttccac ttttcgcaac
    14521 agttttatga aaggacatat tgcttccatt gctgaaatct gggatggaga tgctccccat
    14581 tttcctaaag gagcaccggc tcaggcatgg agtgtagctg ccttatacaa tattgaaaat
    14641 tacatccttt ttctgcagga gggtaaatga aaattctaat gttcacctgg gaatttccac
    14701 ctctgatttc cgggggttta ggtatggcat gttatggaat ggtgaaagct ctgcttagtc
    14761 aaggaataaa aatagacctc gttttaccaa ccaaagaact ggtttatttt cctttaaggg
    14821 aagaaagtga tgccgatacc cttcctgccg tgtttattga accggaaaaa cacaaggaat
    14881 atacaaagcg gactttttct actttgcacg aacgcttgga atatatcgga gccagcaccc
    14941 atccggaaag ttattttaac ttgagtgaaa ttaaggacta tgtaagttcc attaaaaaaa
    15001 ccttctggct tactgaaact gttaccaccg aagaacataa tatctggaaa gaaatgactg
    15061 ctcatctcat tggtgaagaa gatttaattc ggaaagtgca ggaatataca ttgagagcaa
    15121 gtcgttttgc cgaaattttg gattatgacc tgattcatgc gcatgactgg cttacttacc
    15181 ccagtggaat tttagccaaa cagatttccc gtaagccatt gattgtgcat attcatgcca
    15241 cggaatttga tagagcaggt ggtcctggag atgaaagaat tcataaaatt gaacatctcg
    15301 gaatgaatta tgccgataaa gtaattgccg tttctcaata tacagctcag atgattatga
    15361 gtcgctatcg gatagatacc ggtaaaatcc gcataattta taatgctttt accgtttcgg
    15421 aaactactct tcacaacaag aaacgcattt ttaagggtcc tactattctt tatttaggaa
    15481 gaataacttt acaaaaaggt cctgattatt atctggatat ggcagataag gttctgaaag
    15541 ttcatcccga agcaagattt atccttgctg gaacaggaga tatgcagcgt cagattttgc
    15601 gtcgttctgc cgccttacgc ttgaaaaacc gttttctctt cactggtttt ttgaatcgta
    15661 aacaggttga acgaattttg caggcagctg atatttatgt tttaccctct gtttccgaac
    15721 cctttggtat ttctcctctg gaagcaatgg cttatggaat taccgctata atttccaaac
    15781 aatccggagt tgctgaagtt gtgcataatg cctttaagat tgactattgg gatatagacc
    15841 tttgggctga aaccatcaat catttaatag aaaatcctga agaatgcgct aaaataggaa
    15901 tggaaggcag aaaagaagtc caaaaaattc agtggaatga agccgcggat aaaatccgtc
    15961 agctctatgc cgaaacgctt tctgagtata gcaaacgaat ggaattataa ggagacagcg
    16021 atgttaaata ttgtattcta ctttcaggtg catcaaccct atcgtctaaa tcattttaat
    16081 gtgcttgata tcgctcataa tgccaagatt ttcaatgata aactaaatgg cgatataatg
    16141 cgtaaggtag caaataaatg ttatttacca actaaccagt tgcttctgga gcttattgaa
    16201 aaatatgaag gcaggtttaa ggttgctttt tctatttcgg gcactgcttt ggaacaattc
    16261 aaactctatt ctcctgaaac ccttgattcc ttcaagcgtt tagtggatac cggttgtgtg
    16321 gaattattag gtgagaccta ttatcattct ttggcttttc tttttgatac caatgaattt
    16381 cttgatcagg tttatctcca tcgggacttg atgcaaactg aatttggcta ttatacaacc
    16441 actttccgca atacggaatt aatctatcaa gaccgcttat ctgatattat ttttgaaatt
    16501 gaaggattca agaccattct taccgaaggt gtagatagaa ttctccagtg gcgcagtcct
    16561 ttgtatgcct ataagaatta ttccaagaat atgaatttgt tgcttaagta ttatcaattg
    16621 gcggatgata ttgctttccg tttttccaat cgggattggc ctgaatatcc tttaaccgta
    16681 gataaattcg tgcattggat agaccagctc accttagcgg aaaaaggcgg aaaaaacctg
    16741 tttctgaacc tttttatgga ctatgaaacc ttcggagaac atcaatgggc ttccaccggt
    16801 atttttgatt ttatgcgtca tttccccgat gctgttttag aaagagaaca tttgggtttt
    16861 gccaatccta aagatgtttc tcatctggct aattatcaac aggaatcgct ttcttttccg
    16921 gaaccggttt cctgggctga tgaacaaagg gatatttctg cctggctggg aaacgaaatg
    16981 caacaaaatg ccagtgagac cttttatgaa ttgctgaata ggattaagga aaagggagat
    17041 gaagagttgc ttagaactgc tcgtatgctt agcacttccg accattttta ttatatgtgt
    17101 tccaaatatt ttcaggatgg agatgtgcat aaatacttct ctccttacga ttcaccggat
    17161 caggcatata tctattatat taacgctctg gcggaactgg aagagcttct tttaaggtaa
    17221 tgcaatatga aaggaaaaat tttgattctg gaagatgaca tagttctttc cgaacaaatt
    17281 gctaatgtgg tgaagcacta taactatgaa gttatgcaaa ctgttaatag cgatcagttt
    17341 tttgaggtct tacgccaatt tcaaccggat gtgattctgc ttgatgtttt tttagttggc
    17401 tccaaactga acggtatcca ggttttgcaa cacttaaaaa acgatatgga tttgaattat
    17461 aaagtgatag ttatttcggg agaggtaact tcttctcaaa taaatgaaat ccgcacattg
    17521 ggtgcttatc attttattga aaaaggttct gccttttcca tcaatcaatt gctgctgcat
    17581 atagaaaatg ccatcactct aaaaagacag gaagaagaac atattggctt gcagatagaa
    17641 tatatcaacc tcaaaaaaca gtttaccaga agttttccct ttatcggtga aagtaatgct
    17701 attaaaaaag ttagagaaca aattatccgt cttgcctctg tggatgaaga tattttcctc
    17761 attggcgaaa cgggaacagg caaagaagta gctgcaaatt attactacat atattctccc
    17821 cgttttggca aaccctttca taccgtaaat tgcagtgctt tatccgaaac ccttatagaa
    17881 tcagaacttt ttggtcacat aaaaggttct ttcaccagtg ctgatcgcga taaaaccggc
    17941 ttttttgaag aatgtactaa tggcattctt tttttagacg aagtaaccaa tctttcccta
    18001 atgtctcaat ctaaaatcct gcgcgctatt gaaaacaaag aaatacaggt tgtaggtgga
    18061 aacttgaaaa aggttgatac ccgccttatt tttgcttcta atgcgggctt ggaaaaactc
    18121 tccgatgaaa atattttccg caaagacctg ttttatcgca ttgaaggtaa cattgttgag
    18181 cttccacctt tacgggaacg agatgatgac attctgcttc ttatttgtta tttcctcacc
    18241 agtttttctt ctcaataccg cgttattgac cgtttagacc tacctgctct gaaagatgat
    18301 ttacttaact attcttggcc tggaaatgta agagaactgc gtaatttttg caagttcatt
    18361 atgattaatg aacagaaaat aaataataat gttattaaaa aacaccttca acagaaaata
    18421 aaccaattcc gcttttctaa aaactatagc agcgaaaaat ataccaaaat tcataatctc
    18481 aaggatagtg tcgcttcttt tgaacgggat tatcttttgt attatttatc cttgaacaat
    18541 tggaggattt cccaaactgc agaagcaata ggacttgaac gcaccacttt gtataaaaaa
    18601 atcaaatccc tcggaattaa tcatctaaaa atggaggact aaaagtgctg caggaaaacc
    18661 tgggtttaaa aatcctctcg ctattaatag ctgtttttgt ttggttgcag tccttgttga
    18721 tttcagaaca gaaaagcgta gttaatttac ctgtagccct aagttccatt cctaaaaaca
    18781 ttactttaga taatgttccc aatcacatac ctttcaatgt gcgtggaagg ggtttggaaa
    18841 ttatcaaact aaaattttcc cgcaccaagg tcttgcttga tgccagcaaa attaaacccg
    18901 gttccgatat tctttctgta agcgattata ctattgacct gcaggaaaat attcaagtag
    18961 aaattttagg tccagtagag caaagggaaa tttcggttta tgctgatgtc ttccaccaga
    19021 aaaaagtgcc ggtagaactt tctttcgccg attcatatac ccgtaagcat tttactgctc
    19081 taaattatac tattattcct gaacaaatta ccatctccgg tcctaaaagc aaagttcaac
    19141 aaattgatcg cgttactacc gaacctgttt cccgcgaaat gcttgaacag cgtgaagtta
    19201 ccttaaaaat aaaccctttg ggaagtgagg tctccgcttc tgaaaaacag gttaaaattt
    19261 atctctccac ttcctatatt accaccaaaa tttatcataa tctacctctt accgtgccac
    19321 ctggaaaaag ctctatacct tcatctgtaa ctgtaaaact ctccggcaat tctgatattt
    19381 tacaaaacct gaagccaaca gaactcaaag ttagtattgc ttcccaacct gatgaacagg
    19441 gtttttatcc tgtccttgta gaactacccg atacagtgaa attgattgct gttacacctc
    19501 aaaaggtgcg cctgaaataa atgcctgttc aatatatttt ggcttttgag tcctcctgtg
    19561 atgatacctc tgtagccatt gtggatacgg attataatgt aattgtaaac cttatttcca
    19621 gtcaaccgga acatctggaa tttggaggta ttttacccga actggcttcc cgcttgcatt
    19681 taaagaacat agttacctta accaaagtgg ctcttaatgc ttctaagctt aacttacaag
    19741 atatcagtgc aatagctgtt tcaatcaatc ccggtttaat tggttcgtta attgtcggtc
    19801 tggcttttgc caaaggactt gcctggagct tgtctttacc tttaataacg gttaatcata
    19861 tcttgtcaca tatttttgcc aattttattg aacataaagc tgttgaaccg ccttttttag
    19921 cccttgttgt ttccggaggg catacagaac ttgtccattt tgatacctta accactttta
    19981 ctgtagtagg aaaaaccctg gatgatgccg ctggagaaag ttttgataaa gctgcaaaac
    20041 ttcttggttt gggctttccc ggtggtcctg ctattgatga attagcccaa aaaggcaacc
    20101 ccaattttat taaatttcct cgtgccttac cccaaaaaaa taacttcaat ttcagttaca
    20161 gtggactaaa aacagctatt cgcacctggc ttgtaaatca gaatccggaa acgcttcaag
    20221 ataaacttcc cgatatcgct gcttccgttc agcaagcaat aattgaaccc ttagtgcata
    20281 aaacagtact ttgggcaaga cagcataaaa taccctatat tcttttggca ggaggagttg
    20341 cagctaattc tgctttgcgc caacaactaa cagcaacatc agcaaaatat ggaatcaaag
    20401 ttttctatcc atcaaacgct ctttgtatgg ataatgcggc tatggttgga gcagcagcaa
    20461 ttcctaaatt tttagcaaaa aattatgctc cactttccat aaatgtctcc tccctcaaag
    20521 gaacacgcca agtataaaaa taccgctcaa ggaaatgaaa aatgaaaact ggaaagtttt
    20581 aactcacaat tttgccactg ctctcacaga aaccacaaac atcaatttat tcacacttac
    20641 aaccaaatta ttactagtct cttcatttca aaattgtgag ttaaaacaag aacccaacca
    20701 tctataccct ctcaaccttc tcactaattc tctttgtaac aaatggagct aaatggggga
    20761 cttgaacccc cgacctactg attacgaatc agttgctcta ccagctgagc taatttagct
    20821 cttaataatt ttaaaaattg attgttctgc gggagaatca ataaaatatc cctcagttat
    20881 gcggatatca tccttttctt taatagtgca ttgagttccg agttccattg tataataggt
    20941 tgaactaatc aagcgtgcat agaccttaca aggacctgta agatttcgtg gataccaatt
    21001 ttgagccgaa gagaaagtaa catagccacg aatatcggat tctttcaccc attccggaat
    21061 aggaatagaa tagcgtaaag tgaattttat tttgccttcg tagtttggca gataaaaact
    21121 gaaacaattc aagttactat cttcattcag catttccgga tagggaaaca caggattgaa
    21181 atgttccgga acgagtttag tagaataaac aggaggaaca aacttatcat taatcagaaa
    21241 atactcaatt ctggcatcct tagttaaaaa taaactgtga tacactgcgt caggtagaga
    21301 atccttttcc acagaaaaat ccgctgtaat ataaactctt tgcataggag catagacacg
    21361 tagttcaaga ttaatttctt taggatttag taaggcaaaa tttatctttg gcgtgtctgc
    21421 ttcttcagcc ataaggcaaa aagccagcac caggagaact aaggttaaaa gaaatttacg
    21481 cacttttcag ctccttaatt ttgttttatt atttaaccct ttattattag aggtaaattt
    21541 cgtcaagaaa attccctttt atttcattag aactgcttta cgaattacct tgctattgcc
    21601 attttccagg taaatgaaat aaactcctga gccgacggaa cggttgtcaa tatcctttgc
    21661 attccaattg aagcgatgaa ttcccgcttc cagttctcct ttatacaatt ctttcaccaa
    21721 ttgcccttta atattatata tttttatagt agttttgcca gatacaggta aaacacaata
    21781 tattatagtt tcaggattaa aaggattggg gctgatattt aattccggtt tcatcttgga
    21841 aaaatggttt gaggtatttg ctcgttgaac tccaaaactg cgttctacaa gggaaaaact
    21901 cattgacttt ggggtatcgg cagttcgcgc ttgaatccag taataattat taggccaggg
    21961 tatatttatt aagtccgctg tagcagtcca ttccgtccat tcaatcaaac ccacattgtc
    22021 aaataaagcg attgctgtca ttcctgtgct gccagtcatt tgtagacgga tttcataata
    22081 gaaaacatcg gtaggaaggt tcaattcttt atagtaatat gtccaatcgt ttgttcctat
    22141 aatgttttcc gttacataat ccgtatagga cgcaactgct gctgttctgg atgtataaaa
    22201 tctgatggaa atattggcaa gagatacatt ttcggttttc agccaaccgt gcagagtgta
    22261 ttttttggta ttatcataga ttttacaacg ctgggaaatt gtagaagtta ctgaacctgc
    22321 tccggtagta gtcagttttg cactacgctc tccatctata aagtccgttg tagaataagc
    22381 aggtggaatc caaagagttg agccctcatt ttcaaaatta ccaaaccaga tatgttcctg
    22441 acctaagcga aattgatatt cagcaggcgg ttcaacatta ataatattgc taatagaacc
    22501 tctccggggt aatttaatag gtgaagtata actcatcccc ccgctgtgaa acagtaaatg
    22561 ttgccaggaa ctgaaagcat ttgtagtaac gggtggattt tcatcttcca ttaaaactct
    22621 tgcttccaga ttttctttat ctaccataac gcgcgtgtta aaatccatag acctgctggc
    22681 aatataatcc aaaatgtaaa cagccaattg ccctgttgcc ggttttggaa tgtattggtc
    22741 tatataaaac ggttttatgc gaaagtttct aaagccgtca aaataggcat ctgcatacaa
    22801 aatcattgtg ggcatcgttt ccggataatc caaatcaaaa acaaaatttc ccagagaatg
    22861 ggcaatcagc ttattttgat ataattccaa tccctgaatg atatgcggat gatgaactat
    22921 aaccaaatcg gcaccgcttt caattgcact gtgcctgatt tctatatccc attgatgagg
    22981 aacatcattc cgatagaaat agtcctcatc ttccgtatcc tctaaaaagg gattgaattc
    23041 cttatcataa ccggcacctg gagcaataga atattcacta cctccgtgca tttcaacaat
    23101 tttaaggtcg gcaattcctt caactgcttg aagttgctgt tctacatagt aaggtgtcat
    23161 ataagcaaaa cccggtttat tgtatcccgc ttgtaaaaaa ggttgagcat tattatattg
    23221 tcctgttctg tccgaactac gtaaaaaggc aatattcaaa ccccggcaat ggtaaaaggt
    23281 aggtaaatat gcctcgtaag aattagctcc gcaaccgcta tatttaattc ccgagttact
    23341 tagcaatccc tgcatttgct gatatgcttc aatgccataa tccatagtat ggttattagc
    23401 tgtgctcaca acatcaattc ctgcgtaaac aagaccgctt acattagccg gattgcctct
    23461 gtaataaaca cttttggtag gatgatgaac tcccacattg gctaaaacaa cttccaaatt
    23521 tgccacacta atatccgctg cctccccaaa atagggctta gtaggcgaaa atatggcatt
    23581 aactcctaaa gtaggaatta tacctccggg attttcatat cttctggcaa gcattatgtc
    23641 ccctacaaaa ttaagggata caggcaaatg ggaagcacct gtttccaata taacttcagt
    23701 tgaagcaaaa ccaacttttc ccgtatcatc agtcactttc agtgttactg tatagggata
    23761 agcggaagca attgtatata aatgataggg attgggtaaa ttgctgaaag tggaatcacc
    23821 aaaagaccat tcataagtaa atgtatcgct atcaggatca ataacattgc tatagaaata
    23881 aaccgcggta ttttttacgg gataagaagt tgatatgctt acagttggcg gaacaggaag
    23941 gtcggaactg atgtctatta tattgtcaaa ccacacactg cgagaggaaa ttccatccaa
    24001 atcgttaata taaattatgc cgttgataac aggaagataa ccccaaaatg cctgccaatc
    24061 caatgccaga ggcaattgaa aggtattcca gacaccgtta ctaaaagcac cttgataaac
    24121 tggaatccaa acctcaatat ctaaaattct actccccgca aaactataaa gaatattatg
    24181 cacaccatct gtaaaaccaa tgccttgaat ccttgggttt gaactggttt ttacttgaac
    24241 ctgaattaca cctgtgctgt caatagcata aggagttatt tgctgtaatt tgtaacaatt
    24301 acccgttaat ttaagacaat aggaactgct atcaggagta gaagtatcta aactccaggc
    24361 atctggctgc aaatcctctt caatgctcca ggagtttaaa gaaacaacac cactttcaaa
    24421 atcctccaaa gtcgtatagc gaagagcaga tagggaacta tagacgcaca ttagaatcac
    24481 tatgctcatt aaaaacttct gcagcattat ctcctcctct gttttatttc ttcatataaa
    24541 agcaattttt agaccagata aacggatttt ttttttgaaa aaaggaaaga aagttttgac
    24601 tcacaatttt gccactgccc tcacagaaac caaaaccctc aatttatcca cacctacaac
    24661 caaattatta ccggtctctt catttcaaaa ttgtgagtta aaacaagaac aaccatataa
    24721 accttctcaa ccctctaaac cctctaaacc ctctaaacca ttcaactctc tcaaccatct
    24781 caaccatata aaccttctca accctctaaa ccttctcaac ctcttttaaa ttcatttttc
    24841 attct
//
LOCUS       DAOOZV010000026        24272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2218_length_24272_cov_53.995953, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000026 DAOOZV010000000
VERSION     DAOOZV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24272
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_24272_cov_53.995953"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLW08_04050"
     CDS             complement(<1..553)
                     /locus_tag="PLW08_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HPL51806.1"
                     /translation="MKNRPVIALAGNPNVGKTCLFNALTGAHQSVGNWPGVTVEHKSG
                     ICNYNGKSYEVVDLPGIYSLAGGSPDELVARNYILAEKPDIIINLVDASNLERNLYLT
                     VQLMELGAPLLMCLSMIDIAEHNGIYIDTEHLSSHLGFAVYPLVLNKRIDVSPILKKV
                     EEYLIQPPVPATIHYDEVVEKHLQ"
     gene            complement(543..1001)
                     /locus_tag="PLW08_04055"
     CDS             complement(543..1001)
                     /locus_tag="PLW08_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPL51807.1"
                     /translation="MEEYAVIFNQYLNHKGLKLTKPRRCILDTVFSMHTHFNAEELYD
                     KIRGKTSSISLATVYRTIPLLLDAGLVQRALRSEGRERYEHIFGHPKHIHWLCRNCGD
                     LMETDLNTLYPILEKQAQDLKFKPESIELNVKGLCWKCTSIENETQSDEK"
     gene            1461..1751
                     /locus_tag="PLW08_04060"
     CDS             1461..1751
                     /locus_tag="PLW08_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL51808.1"
                     /translation="MQELITELKSCYPNPFQTSTSIHYSVKEAVPVEIRIYKARGELV
                     HKLVNDNRVPGNYHIVWDGRTQNGTLALSGIYFYQMKAGNFNSQIKKLLLMK"
     gene            complement(2383..3831)
                     /locus_tag="PLW08_04065"
     CDS             complement(2383..3831)
                     /locus_tag="PLW08_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51809.1"
                     /translation="MFKYYCISICIIFISLLSAYSIDETLIEISIEKEAMARDNVVCE
                     NKIAELRNNDPDFLPQDMFESDTKYLMRICKSAPKIIAIRDQYIKPHLENLNCLRKTI
                     GGVGDIKIQLDNYDANTEKYSLYITNEKYPHLNCRALLSIPSDEARFLYENFDKFDYF
                     ACGYISYDDTIAISYIDIYNKEDPICQYEVDTLKPYYFYSDFSYSDFSYDGEIFACSN
                     NKSVYVLKTLPDSTICLYKNNFPNKINCISLSPEGKYLAVGAYGIEPTKKDFWLINVQ
                     TKEVILSESFDGGIYSVDISPDGRLVAVGGGFSYTNSDARIYDIETGKMLWDFSGYEC
                     IKGVKFSPNQKYIANIGKELNVYDLTTLDKVINVSTSNCFFALDFSPDSNILVVGGKY
                     EGFAFYDMNSKMLTNSYENNKHVYSLSFDFTGKLLAVGYEDLKIISLKAMLYEREFKT
                     FGGLYKPGSIYVVSHGKAYISFYPNSAGYEEY"
     gene            complement(6259..6936)
                     /locus_tag="PLW08_04070"
     CDS             complement(6259..6936)
                     /locus_tag="PLW08_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPL51810.1"
                     /translation="MKTNAYLLVLLLLLLVAGLQSQTLTQVKLGVIGGLNFANFSGDD
                     IEDLDDEGFDIEGRTLGHIGIIGNATVLQNLSLQGEIAFSMNGSKWEANFCDEYEVYD
                     ATWTHSINMLQIPVSAIYTLNLPELAIKPYLGAGISASIPVSSGLSVELGGQDEDFDY
                     DDDDYIELSSFVLGYQLNLGMEYKRYFCEIRYERSITPVFDDKYVDDVFYKNLKLLVG
                     FRFDSNF"
     gene            7155..9077
                     /locus_tag="PLW08_04075"
     CDS             7155..9077
                     /locus_tag="PLW08_04075"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HPL51811.1"
                     /translation="MQEEMKESMQEQVSEEIPSSEEGTPAVLEGKRKKKSALIPVLIV
                     LFIILVVIAVYYFATSSPQIRHISKNALVVSKVNLPQLLKESGTMKKGEIDEDLISAL
                     EEYNLEALVKDPRSTGLITTKPSYLFGEYDPKKNLPYIFMVMPIANAEKVYNFTKNIA
                     LPGGNDLSVSKKSNIYEIDLQGLSCMWTGKSMLIGMTDPNNDNDLSKKVKTYLTQDKS
                     DCILANKKFSRSKLRGHDITLWANLDGMARIAQKGLKDAQGNVERLISQRKQRKMYSD
                     YYFNTNLPYSYSYWEIMDQFQVDDPKNVFESVLLGIAYESKIPMKTVIDLPTELYNTY
                     LSFSADFDKGKMVSSVKFETTPERQKKLESIIADAVNVKDLYPYVPTEGLIMLSSVKT
                     NYPKLYTLTEKRIDKLTKSVDNPEISKLMKHLNKLADGSGIIAVNILDEKEAPFVTLI
                     ASVKDNKGIQELLKDMAREGHLRKDGKYYYLDELVIFFEDNVMICTTNHNNYKKSNRN
                     LEGTLARQMNKNPFNMTMDFTALKDLTDDLSDEEEDALDMLNKLNALVKTVGKTSNEL
                     KVELTFSDKKHNCLKQVKDYLSDIDNFWDEITKEIMYSVSYRAERDDTYPELNYEIPR
                     EVIEETGPVPDFDTLY"
     gene            10088..11419
                     /gene="dnaA"
                     /locus_tag="PLW08_04080"
     CDS             10088..11419
                     /gene="dnaA"
                     /locus_tag="PLW08_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPL51812.1"
                     /translation="MDQDIWQNILDKLEENINPQSFKTWFSDTRLVDMNDRELMIRVA
                     TQFSANYLNQNYKEVLGEISYGLYGRKYDVQFITQPHRNHNKEKDVQDYSIKRVSANV
                     RLNERYTFDEFVVGKNNNFAYSAAKAVAESPGYTYNPLFIYGESGMGKTHLMQAIGNY
                     ILKEGRNCSIYYITSEAFTNEMIDGIRSNKMPEFRAKFRKVDLLLMDDIHFLSRKEGT
                     QEEFFHTFNALFENKKQIVLTSDRPPIDIPDLEKRLVTRFESGLLCDLKSPDFETRVA
                     ILRKKAEPENVVLSDAIFNFIADNITSSVRALEGSLIRILAFSSYNKLNPEDIDINLA
                     AEILSDMISEKVHSITLDAITEKVCECYGITPVQLLEKTRKPQVAFPRQVAMYLANYL
                     IPQLSLKDIAEYYNRKDHTTVLHAKKMVENQFREKEDFRIQLEEIIKNLKK"
     gene            11840..13054
                     /locus_tag="PLW08_04085"
     CDS             11840..13054
                     /locus_tag="PLW08_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51813.1"
                     /translation="MELLPKVLKLLLCIIILVIMLSGCTDKKNLTGDNFSDIRAITIE
                     DKPGIIMGFSFPAETKEEITGKEAKLLAGNYQNAVAICLLRFTELPSPITETDSCFLT
                     INISKRSALPRNPLILKLYKLNSVWLDSLNLNDISEDDLSLLVQYTVADTVGSSGKEI
                     TFNLSSSEVVNWSNPDSTGWNFVLKTENEGWVEISSLEGTNKPTLNFKYKTETGGEWI
                     NYNKSPAKDTYILEAPETNPSVLWKISNLNSSRLYIKYEPTYTLFKDNEGNTLTDIQL
                     KRLTVNKAEIVLHIKNNDYYNSSTSYSLYPFNVVKDSIDTPVTLVKSDYEKLAYIDYS
                     IGFIKGDSLTVDVTPIIQAYTSGEKIPKGIMIQSTQERRNFGTLEFWDCNESTPAEKK
                     PYIKITYTPPYL"
     gene            13056..13856
                     /locus_tag="PLW08_04090"
     CDS             13056..13856
                     /locus_tag="PLW08_04090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL51814.1"
                     /translation="MKRFALIPLILILLFACTKKEEKGIALAQVDKEILYLDDFKSTF
                     SVEDWENLSPEIRKKYIEDWVNLTLLAHYADKQGLNKDQAVKQKINYASKKVKANALI
                     AKRLSEIEVSEDQLFNYFRLHQAEFQKNAVEYNIRRIALMDKISAENVLQQLSQGMSF
                     TEAVQRYSTEELKNRGGFMGMVSATGPDSLFWLKARELKENDYGLVYKEPMWYVFQIV
                     ESKITPQEANFEDYRAEIKRKVLLEKQDQVYQELIKEIKSQTSEIYYY"
     gene            13871..15127
                     /locus_tag="PLW08_04095"
     CDS             13871..15127
                     /locus_tag="PLW08_04095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL51815.1"
                     /translation="MRKTAIILILLVLTLSLSAELIDKIVAKVGTDIILLSDLQKEMV
                     QMQSAGLLEENTDPRDVLDEMINQKLIIQKAKDLNITVNNDEIKAMAENYLKKVKAQY
                     PSSSAFAADLKKSKLTESDLLQLYKDILTEQALSDQILKKEIINKVSVTEAEVINFYN
                     ATKDSLAVKPVSWDLGIIFREIKPSQESKEAKLAEIKEIQRRLKNGEDFATLASTESD
                     CPSKEVGGDLGFFKRGQMVKPFEDAAFALQLGEISDIVESEYGYHIIRLEEKRGNEIR
                     ARHILKALTPTAEDSLRERQLMEEIRNRYANGESFASLAREYSMDQESREDGGSLGEF
                     TERDLPSLFATQILQTPVGEMTPVLENQGMLYIFCRLKEYPPRIYKYEEVKDQAREMV
                     LKRKQIDAFNAWIENLRREAYVQITL"
     gene            15124..15621
                     /locus_tag="PLW08_04100"
     CDS             15124..15621
                     /locus_tag="PLW08_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPL51816.1"
                     /translation="MTAEKPQKLNIYTFLATLCGIGFIPVMPGTFGSLLAYGIYMLFS
                     GSPFQGKALFYALPALIVLCLIAVFLSTKAEKTLGRDNGSIVIDEFCGYYVSVLFLPH
                     SWLIGLYAFALFRVFDIAKPFPIKKVQELPGGWGVVTDDIIAGIYTNILIQILTKIYP
                     KFFGL"
     gene            15630..15971
                     /gene="yajC"
                     /locus_tag="PLW08_04105"
     CDS             15630..15971
                     /gene="yajC"
                     /locus_tag="PLW08_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPL51817.1"
                     /translation="MNYILLQAAQKGTTQQGGAGTTLIFMIIMFVILYLLLIRPQQKK
                     AKETQKMQDSLKVNDKVITSSGIYGRVVSIKPDKGIVVLEIDDTNKVRIDIQKNAIIG
                     VVTTSEPEPEK"
     gene            15968..16528
                     /gene="def"
                     /locus_tag="PLW08_04110"
     CDS             15968..16528
                     /gene="def"
                     /locus_tag="PLW08_04110"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPL51818.1"
                     /translation="MKYKPKILPVRLYGDDFLRKKLPEIDYNTPGLQEFIEDLIYTMY
                     ARDGIGIAANQVGSFYRMIVIDPEQDNKLNKKSPIVMINPVIENREGEVVYEEGCISL
                     PDVFADVSRSKKITYSYTDRMGNRITETAEEIKAVVIQHEFDHLEGILFIDHLGTLDR
                     LKIMHKLKKLQARAVNGQNILEGLTL"
     gene            16528..17472
                     /gene="fmt"
                     /locus_tag="PLW08_04115"
     CDS             16528..17472
                     /gene="fmt"
                     /locus_tag="PLW08_04115"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPL51819.1"
                     /translation="MRYLFIGSSDYGLPALNKLRQNGYEPSLIISQPAKPAGRNLHLT
                     PTPVSQYAIEQKLPLFTPVDINSAESIAKMAEQKADIIVTAAFGEFINKKIRNLCPFG
                     AVNLHPSLLPKYRGASPIQSAILNGETETGTTISLVSAKMDAGPILAQTKLSIAENET
                     YSELKERLAEQAGDLLLQFWKKLITEKSLCGTKQDESKATYCYKITNKTCQIDWNKRT
                     EEIHNQIRAFSLTPGAWTLFRNNKLKILCSEKTEEPANGEPGLICKIEKKRGFFVNCL
                     DFKLLITKVQPAGKKVMEAVAFINGARLREKEKLGGEK"
     gene            17469..18221
                     /locus_tag="PLW08_04120"
     CDS             17469..18221
                     /locus_tag="PLW08_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HPL51820.1"
                     /translation="MKKEYFSIIKFPVLISFSLLIITLIFFFIVLGSYHRLGLSVWGN
                     VLYTILFIVIVILIGTWILALISTHRKIKPLWLRHYLSWMLVNIYYPLARVFGRICFQ
                     KMDTIQESFLYFNNEIVVTNYQGMHSSNLLLLLPHCLQSSNCKIRINNSIIECQECGG
                     CDMAKMKALTKDYQIKTAVASGGSLARKLVNDTHPDIIIAVACNRDLTEGVRESWRYP
                     VYAVLNERPNGPCFETTVSPAVIDFAIHKFVQ"
     gene            18234..19070
                     /locus_tag="PLW08_04125"
     CDS             18234..19070
                     /locus_tag="PLW08_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPL51821.1"
                     /translation="MKRFNLFTIILILLLNAFITMALINKYGNKTPETDTFVSPGTSQ
                     PSKASSAKRINSITEAVKAVEPAVVSINVIKTEYVRAVSPFGFGFFDFFGSIPLLRQV
                     ESIGSGVIYDPKGYIVTNAHVVSGATQIKVILPDKREFSGIVAGIDEIHDVAKIKITG
                     NNLPVAEMGNSNDLIIGEWSIALGNPYGFLMNDSKPSVSVGVISAVNRNFAARQDRRE
                     YKSMIQTDAAINPGNSGGPLVNINGEVVGINTFIFSESGGNIGIGFAIPINSVKTILA
                     KI"
     gene            19067..21790
                     /locus_tag="PLW08_04130"
     CDS             19067..21790
                     /locus_tag="PLW08_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPL51822.1"
                     /translation="MNRKYTLFLLILLSCGLLFAQTALSCYDIQYNPEGGGDSPYLDQ
                     IVTVQGIVSGVTYYSGSGYNNYGFFISDPTGGPWSGLFIYNQTYHPALGDLVRVTGTV
                     KEYYGLTEISQVSSLLVLSQGNPLPAPTEINTGSLNNFSSGEQWESVLVKVVNVTVTV
                     EPNSYQEFYVDDGSGQCQIDNQFFGSNHSWNDITLGQVYSEIIGIVDYSYSYYAINPR
                     SETDMISGDNTVTLAIPHQSVGLHSSVSVPINAYRIETAQNYQSYSFSISFNPLILNY
                     ESINTASTLSQAGNVIASAESGVLSVSYQSNSILSGQGILLKLNFYAANTGTTSLTFS
                     DVIFGADVIQSCINGSVTVNSSYNSPGDTLTVIQRPLLNIPEIVVPGETLKITCLAPQ
                     NTSNWNAWLRHNNKRLNLPVTNSQWLTLPNRWELTTTVPSVPVYELYDLEVSASGGIS
                     DITANAVQVIPSRKNNYYFVHITDSHMPNRLYYPNAGFDADSTAVEDFRAVMDDINII
                     HPEFVLFTGDLINEGELEGFANQYWFGWTQKLLTEFEVPVYVTAGNHDIGGWNQTPPP
                     AGSARKNWWKYFGWSWLNNEDLSFPYHTQDYYFTYNNAVFIGLESYDNYDYWRPAIYG
                     ETSYTDQQIAWLNNTLSLFPNYKKVLFHHYDFKEQLFLSSLGIDLSLWGHIHSNNGSI
                     YTYPYNLATRSVCDGNRAYRVIRVNNEQLTPYATIYAGATGNNLSVQFYPSNYGVADS
                     VKAIIINNQPLAFENAQLKFIMPQRDTGYNVSGGTLTQVDKSGNYNICYVRVSLSANS
                     TSTVTVTENGVEISDPVQVPVVFTIKSVYPNPLVKQGNLQVISDKTFPELHLQLFNLR
                     GQLIYQECLKGISQGESHLSFTLPDSLASGIYFLRFREDKTQIHKILILQR"
     gene            21797..22804
                     /locus_tag="PLW08_04135"
     CDS             21797..22804
                     /locus_tag="PLW08_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL51823.1"
                     /translation="MPEKRPQTSKLLYLPWIIKHLSYYLKLNTHILKKGVPLKEILIL
                     KYPFWFKDAKNPPLLSVDITDACNLRCIYCNNPFFPYPRTMMNQDVFKALIERLKRHP
                     VSRIRISGGEPTLHPQFDTMLKEMSYYCKYLSIITNGQWQNPEVNEQLLNCGLSFIEL
                     SMDAGGKEIYEKSRPGADYDLFFQNLKQLYKLRNQYKVDLIIRIRLMVRPSTMDKEKE
                     DSRFLRKFCDCVLPQHILQHPETPSQEDVFIQNSILQNSLPVCTVPYRDLQIRPDGLV
                     PLCPAKGCAINPEDRIFIGDIQKDEILDLWNAPLLKQMRDAHRNRKGEILKLCRNCHY
                     S"
     gene            complement(22970..23758)
                     /locus_tag="PLW08_04140"
     CDS             complement(22970..23758)
                     /locus_tag="PLW08_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPL51824.1"
                     /translation="MSKFYLFCFAVVLVLMAVPLFSAEEDNFLQDYFLSYPETIYAGA
                     TAPFSWQKDNWLQASAIVITGGCLYFLDDDLRDCFQRNRNEVTDDIFTVAKQFGEVKI
                     MFPVMAVTALGGYLTKDEKTIDTSLLCLKSSLLSLAVTQTLKLASQRQKPYKEEGKEF
                     WNGNGISFSRDSFPSGHTTLVWSLAPVLAEQYKNSRWVPPTVYTIATLTSLSRLNDDQ
                     HWSSDVFAGAVIGYITARLVLKNTPRLMLNAQPEINGLSFKYKF"
BASE COUNT         8034 a         4424 c         4369 g         7445 t
ORIGIN      
        1 tttgcaagtg cttttccact acttcatcat aatgaatcgt tgccggaaca ggtggctgaa
       61 ttaaatactc ttccaccttt ttgagaattg gagaaacatc tatccgctta ttcaaaacca
      121 gaggataaac ggcaaaacca agatgggaac ttaaatgctc ggtatcaata taaattccat
      181 tatgctccgc tatatctatc atagaaagac acatcaaaag aggggcaccc aattccatca
      241 attgcacagt taaatacaga ttgcgttcca gattggaagc atctaccagg ttgattatta
      301 tatcgggctt ttcggctaaa atgtaattac gggctactaa ttcatcggga ctgcctcctg
      361 ccagagaata aatgcccgga agatcaacta cttcgtagct tttaccgtta taattgcata
      421 tccccgattt gtgttcaact gtaactccag gccaattacc aactgattga tgtgcaccag
      481 ttaaagcatt aaaaaggcag gtcttaccta cattgggatt tcctgccagg gcaataacag
      541 gcctattttt catcggattg cgtctcattc tcaattgagg tgcatttcca gcatagaccc
      601 ttcacattca attctatact ctccggctta aatttcaagt cctgcgcctg tttttccaat
      661 ataggatata aagtgtttaa atccgtctcc attaaatcac cacaatttct gcataaccaa
      721 tgaatgtgct ttggatgacc aaaaatatgt tcataacgct ctctgccttc actgcgtaaa
      781 gctcgttgca caagccctgc atccaataag agaggaatag tccgataaac agttgccaga
      841 gatattgaac tggtctttcc cctgatttta tcatacagtt cttccgcatt aaaatgggtg
      901 tgcatactga atacggtatc cagtatacaa cgcctgggtt tagttaattt gagcccctta
      961 tgattaaggt attgattaaa gataactgca tattcttcca tcgttttctc ctccttgtat
     1021 tgagaatcat tcacaacttt gcacaaccgg gttttctgtc aagcaacatt tggaaaattt
     1081 atgatgcccc cctttttaaa acaggatagt ataagttgct atctgtagtt tatgaaaaag
     1141 atatacatac tacaagtata cagataatca cggcaattcc cgtaattcct gtctattaaa
     1201 ccatagctaa tcttacggca gaaacatcca acaatgatgt tatactcaac tgaactgcac
     1261 cagaagctag tgaagctggt tataaaatct atcgtgatag caatcttatc tatactataa
     1321 ccgacccctc tatcacccat tataatgata ctggactaac tgcaggtaat tatttatatg
     1381 cagtaattgc aacttatcca accggtgaat ttgcaccagt tacgattagt gtctttgttg
     1441 tatcctgtga tgacgcatct gtgcaagaat taatcacaga acttaaatcc tgctatccta
     1501 atcctttcca gacctctacc tctatccatt actcagtaaa agaagctgta cctgtggaaa
     1561 ttaggattta taaagctcgc ggtgaattag tccataaact ggtaaatgat aacagagttc
     1621 caggaaacta tcatattgtc tgggatggac gcactcaaaa tggaactctt gctctcagtg
     1681 gtatctattt ctatcaaatg aaagcgggaa actttaatag ccagataaag aaactactcc
     1741 ttatgaaata agctaaaaat atcaaaccca tttcataaga aagggaaaga ttgcttatgc
     1801 tttaaacgaa agatattcta agaaatgaaa aggataggaa ttatcattaa aaataacata
     1861 actttctata ccgtagaaga ttcatacatc cttttcattt cctttttcta aaactggaag
     1921 tcattataat ttttttatta ctttataaat tgcaattcat tccctcacaa cctcataata
     1981 cctttgttat caattacaat ctggtaattc ttatctttag ctatagctaa tagattatta
     2041 taatgataaa tattaccatt cctaacggaa ttagctctga acattaatta cggttcgtta
     2101 ttatatcctt gtcatctgat gatcaatcat ctttataaat ctactgttta taaagtaatt
     2161 atctttaaac gattccaaaa taattgctta aacgcctgaa agggaaacac aataataaca
     2221 atctcgtaaa ccactcttaa aatttacgat tctttagcat agaaatcgtg aattatacaa
     2281 aggtcttatt ccaccatctc tattattatg ttgggctctg agttttcaat attatcaact
     2341 ttataatcat tatttatata cttttgacct tttttattta tattagtatt cttcataacc
     2401 agcagaattg gggtaaaacg atatataagc ttttccatgt gaaactacgt aaattgatcc
     2461 aggtttataa agacctccaa aagtcttaaa ttctcgctca tataacatag ctttcaagct
     2521 gattatcttc agatcttcat aaccgactgc aagtagtttt ccagtaaagt caaatgacag
     2581 ggaatagaca tgcttattgt tttcatagct attagttaac atcttggaat tcatatcgta
     2641 aaatgcaaaa ccttcatatt tccctccaac tacaagaata ttactatctg ggctaaaatc
     2701 caaagcaaaa aaacaattac ttgttgaaac attaatgact ttatccagtg ttgttaaatc
     2761 atatacattt agttctttgc caatatttgc aatatatttt tggttaggac tgaatttgac
     2821 tcctttgata cattcataac cggaaaaatc ccataacatt tttcctgttt ctatatcata
     2881 tatccttgcg tcagaatttg tgtaagaaaa acctcctcct accgcaacaa gccgcccatc
     2941 tggactaata tctacagagt atatacctcc atcaaaactt tccgataata taacttcctt
     3001 tgtttgaaca tttatcaacc aaaagtcttt tttcgttggt tctataccat atgcacctac
     3061 agcaagatat tttccttctg gactgagtga aatgcaatta atcttattag ggaaattatt
     3121 tttatataaa caaatagtag aatcaggtag agtttttaga acatatacac ttttattatt
     3181 actacatgca aaaatttcgc catcataact aaaatctgaa tagctaaaat ctgaataaaa
     3241 ataatagggc tttagtgtat ccacttcata ttgacatatt ggatcttctt tattataaat
     3301 atcaatatat gaaattgcta ttgtatcatc ataggatata taaccacaag caaaataatc
     3361 aaatttatca aagttttcat acaaaaagcg tgcttcatct gaaggaattg ataacaaagc
     3421 tctacaatta agatgaggat atttttcatt agtgatgtat aaagaatatt tttcagtatt
     3481 agcatcataa ttatctaatt gaattttaat atccccaacc cctcctatag ttttcctaag
     3541 acaatttaga ttttctaaat gtggtttaat gtattgatcc cttatagcaa ttatctttgg
     3601 tgctgattta caaatcctca taagatattt tgtatctgat tcaaacatat cttgtggtaa
     3661 aaaatcaggg tcattgttgc gaagttcagc aattttattt tcacatacta cattatctcg
     3721 ggccattgct tctttttcta ttgagatttc tattaaggtt tcatcaatgg aataagcact
     3781 tagtaatgag ataaatatga tacaaattga aatacaataa tatttaaaca tctttcctcc
     3841 attttacttt atataagttg agtctcaaat gttggtgtaa attccaactc attgtttctc
     3901 aagttctttg agggcatttg atacccctca atactcacaa tatatgtaag gccacacgac
     3961 cgaatgcttt attttcatct ctttaattca agtcaagtcc ttttttttca tcaaatgcta
     4021 ccttactacg acggctcata tcaaataaac tgccggggct tttttttgaa cagttataaa
     4081 tttctttcat agcacattac actctttaac atatagcctc ttcagctact aattatacta
     4141 tttataataa gaatatcttg tcaagcacaa aaaattgttt tttttcattt ttttgtggaa
     4201 agctataaac acccttgcag tttcattttt cagtatacat aagttccgta gaaatcactg
     4261 cgaaatataa tgggttgccg aactctgatt cggcaaatgc cgtcaggcat attttttttc
     4321 aaaggtctcc gacctttttg gaaaagaaaa agtttgtcaa cccatgacct tggctaattc
     4381 ttcggctttg agccaggaaa aactcgcaag aatattcaac ttcttaacta ctgtactatt
     4441 aggcaaagct agtattgctt actcaataga ctccaaagta ctagtatcta tgctgtgttt
     4501 gtgtcatccc ctttcttact cctttttggg taagatatga gaaaagataa taagtattta
     4561 tagcttgaaa atataaatta gttgaatctc aaatgttggt ttcaattcca acacattgtt
     4621 tctcagattc ttagagggca tctaatatcc ctcgtttctc acaatctatg caatgccaaa
     4681 cgaccaaatg cattattttc atcttcttaa tctatgtcaa tctctttttc atcaaaagct
     4741 accttgctac aaagagctta tctttatagt ccctaactga aaacttcaaa tcggagaaat
     4801 tgggtcgtaa ttatgctatt cagtgatgta agtttttatc accatttctt caagtaacat
     4861 caccgtttct gaaaatttaa tcactgaaaa cttcaagcct ttatcatact tttttcaaat
     4921 cgccatcatc atttttttaa acttttatca ccgaaacttc aaatggaatt ctaaaaaata
     4981 gtattctacc gtaacatttg ataattgcct gtttgcgtat aacaaagcat tttacgagat
     5041 tgaagataac aatacttcac tgattgaaaa ctgcttattt agtataagta ataacatttg
     5101 gagaacatat aatggaaaac cactgaattg gggccaatta gttacaaagt tggtaaccaa
     5161 ccctcaaaga aaagggacat acaaagtcac ttggaatgga acggataaaa ccggtaattg
     5221 agtatctccc ggactgtact tttaccgtat agaaacaaat ggtaagatat tgtccaataa
     5281 aatgatactg ttaaaataga agaacacata aagtaacgca tttaaaagaa aaatcctggc
     5341 tcaatttcac tattcaaaag ccgtattcgt tctccatctc caatgaaact gtggaaacgg
     5401 agtatgagat cctgagacaa ataccgggtt catttctgca tcgtaatcta tctgatcagc
     5461 tttggcctaa agaacaagag ctttgatgtt tccccgggca ttattcagag cattggactt
     5521 ggtatttgta ttatttgggt ttaggttaag agtagaaaat agttcaagag gaagacatga
     5581 aaaaggcgaa tttcagctaa ttgtagctaa ataaggaaaa gcagaaagca gagaaaaaaa
     5641 agattgggca agaagaaaaa aaagattata atatatatgt gaagcatctg gagtgcctgc
     5701 ccgctgagaa agatgtgaac tggtcaaata gcaggcaatg ttgatgatca gcataataat
     5761 ggggctgtcc cgaaactaaa gattagtttt tagacagtta atagaagcga tcttaataaa
     5821 caatctcccc ttgaggagac atttatgtat acccaaaaac actaaaatat gcactttttg
     5881 gaggttgtga gacagcccca tattttggta atatgccttg tctattatca ttaatatcat
     5941 tgaatcgcag aattatattc aaaacaaaaa tgatacagag tggcaaacta aaatgttata
     6001 gcagggcagt gtaaaagtga ttcacttgaa ccttttgtta tatcaaaaat cggcaaaaat
     6061 tgcagttgtg aagttagaaa acagccccaa ttttgttcgg gtatctgcag tttagcaaag
     6121 aaataaattg ccgttcccta tttccctgat tacaaattta gaaaaatcgg ggaaagctgt
     6181 gcgctaagtt attagcaaga aaagcggagg caacgaaacc tccgcttgca atgcacaact
     6241 ggttatgttt acataatatt aaaagttact atcaaagcga aaacctacca ggagtttaag
     6301 gtttttataa aaaacatcgt ctacatactt atcatcaaac acaggagtaa tacttctttc
     6361 atatctgatt tcgcaaaaat accgcttata ctccataccc aggtttagtt gatagccaag
     6421 aacgaaggag gaaagttcta tataatcatc atcgtcataa tcgaaatctt catcttgtcc
     6481 accaagttct accgaaagac cggagctgac aggaatgctt gctgaaatgc ctgctcccaa
     6541 atatggtttg attgctaatt caggaagatt cagagtatag atagcagaga caggaatctg
     6601 cagcatattt atactatgtg tccaagttgc atcatatact tcatattcat cgcaaaaatt
     6661 cgcttcccat tttgacccat tcatactaaa ggcaatttcg ccttgcaaag aaagattttg
     6721 taatacagtt gcatttccga taattccaat gtgcccgagg gttcttcctt caatatcaaa
     6781 accttcgtca tccagatcct caatatcatc tccactgaaa ttagcaaaat ttagccctcc
     6841 aattacacct aactttactt gggttaaggt ttgggattgc agtcctgcta ctaatagcag
     6901 taaaagcaat actaacaggt aggcattcgt tttcataata tttctccttt tttcttaggc
     6961 agaatttcct tctgcgacta taattcctta tcttagcgtt tagttttttt gtcaagatgt
     7021 tttctgtttt tttaggattt aatattagtt ttttggcagc gagaaggaaa aaacttgaca
     7081 taattttaga gaactacata gttgtgtttg tctttatttt tgctgaagaa caaataaatt
     7141 atttggagga acttatgcaa gaagaaatga aagaatcaat gcaggagcag gtttcggaag
     7201 aaattcccag ctcggaagaa ggaacccctg cagttttaga gggtaagcga aaaaagaaat
     7261 cagctttaat cccggttctg atagttttat ttataattct tgtggttatt gctgtttact
     7321 attttgcaac ctcttcgcct caaattcgtc atattagtaa aaacgctctc gttgtatcca
     7381 aggttaattt accgcagcta ttaaaggaga gcggaacaat gaaaaagggt gaaattgatg
     7441 aagatttgat ttctgccctg gaagagtata atctggaggc attggttaaa gatccgcgca
     7501 gcacaggatt aataactacg aagccctctt acctatttgg ggagtatgat ccgaaaaaaa
     7561 accttcccta tatttttatg gttatgccta ttgccaatgc ggaaaaagtg tataacttta
     7621 ccaaaaatat agctctacct ggtggtaatg acctatccgt tagtaaaaaa tccaatatct
     7681 atgaaattga tctgcaaggt ctttcctgta tgtggacagg aaaatctatg ttgataggaa
     7741 tgaccgatcc aaacaatgat aacgacctta gcaagaaagt gaaaacttac ttaacccagg
     7801 ataaatctga ctgtattctt gctaataaga aatttagccg cagtaagctg agaggtcatg
     7861 atataaccct ttgggctaat ttggatggaa tggctcgtat tgctcaaaaa ggattaaagg
     7921 atgcccaggg aaatgtggaa agattaattt cccagcgcaa gcagcgcaag atgtatagcg
     7981 actattattt taatacaaat ttaccctatt cttacagtta ctgggaaatt atggaccaat
     8041 ttcaggtgga cgacccgaaa aatgtttttg aaagcgttct tttaggaatt gcttatgaaa
     8101 gcaaaattcc aatgaaaaca gtaattgatt tacctactga attatataat acttacctct
     8161 ctttttccgc cgattttgat aagggaaaaa tggtctcctc agtaaaattt gagacgaccc
     8221 cggagaggca aaagaaatta gaaagcatta ttgccgatgc cgtcaatgtg aaagaccttt
     8281 atccttatgt tccaaccgaa ggattgatta tgctgagctc tgtaaaaacc aattatccaa
     8341 aactctatac ccttacggaa aagagaattg ataaattgac gaaaagtgtt gacaatcctg
     8401 agataagcaa gctaatgaag catctgaata agctggcaga tggttccggc ataattgcag
     8461 tgaatatttt ggacgaaaag gaagcaccct ttgtaaccct tattgcatca gttaaagaca
     8521 ataagggaat acaggaattg ctgaaagata tggcccggga aggtcatttg cgtaaagatg
     8581 gtaagtatta ttatttggat gagttagtca ttttctttga agataatgtg atgatctgca
     8641 ctaccaatca taataactat aaaaaaagta atcgcaattt agaaggaact cttgcccgac
     8701 aaatgaataa aaaccccttc aatatgacga tggatttcac ggctttaaaa gatttaacgg
     8761 atgacttgtc cgatgaagaa gaagatgctt tggatatgct gaataaattg aatgcgcttg
     8821 ttaaaaccgt tggcaaaacc tccaatgaac tgaaagtaga attaaccttc agtgataaaa
     8881 aacataactg cttgaaacag gttaaggatt atctaagcga tattgacaat ttctgggatg
     8941 aaataacgaa agaaataatg tatagcgtaa gttatcgggc tgagagagat gacacatatc
     9001 ccgaacttaa ttacgaaata ccgcgagaag ttattgaaga aactggacct gtacctgatt
     9061 ttgatacgct ttattaacaa taatatatag tattgagatg ataaatactg ctttgcctta
     9121 gaaattaaag gttacttaaa gaaaattacg ctgtatattt aaaaccgcaa accattttta
     9181 cagatataat aatagaggtg gcaaggaaaa atttgccgcc tctatttttt tatttcccct
     9241 tcttccctaa tagcattgct tactcattgc ttacccattg ctaatgccat ccgtaatgag
     9301 tcagtaatga gtaagcaatg ctattaagag aatggagatt aaagaagatg agctgttcta
     9361 aaaatagtgg actatatttc aagaaaaaat cctgttaagc ttgtggatcc ggttatcggc
     9421 gttctatttt cttcctctat tatatagttt tgctcttaaa aaagcataaa tattttattc
     9481 tttttggtag atttctgttt tcatcggaat gacaaagggc ttttctttca agaaatgata
     9541 tcggctcttg ttctctttac aatataaggt cctaaatctt aacctatctc tttttctctt
     9601 gttctctttg taaaaaaacc ctaaaatatc tgtgttttct ttattcttgg tgctttcggc
     9661 ataaaaatac aaaatttttt tggaaacgat aatagcatat atttatgaaa gatagttcta
     9721 taaaaatagt tatagaaaaa tcgggattag aattctaact tttttctaaa tgagggaatt
     9781 atcaatttta attcttctct aagctatttt aattttagat tttagaagct tgacaaatca
     9841 gagtgaaata tttttttggt gaggatggtt tttttaacaa aattgaaaaa cgcttaaagt
     9901 aaagaaaaga aagaaattta tcaaggtgtt gtggataagt tgtggataaa tcggattcag
     9961 gtagttgact ggatgtctat tttatcggca taacttatta aatttgaata agatagtaat
    10021 cataaaactg ttactgagct ctcagtatac agtttgtgga taaataaatt ttacaggaaa
    10081 gtgacttatg gatcaggata tttggcagaa catattggat aaactggagg aaaatataaa
    10141 tcctcaaagt ttcaaaacat ggttttcaga tacaagatta gtagatatga atgacaggga
    10201 attgatgatt agagtggcaa ctcaattttc ggctaattat ctgaatcaga attacaaaga
    10261 ggttttagga gaaatttctt atggtcttta tggtcggaag tatgatgttc aatttataac
    10321 tcagccccac cgtaatcaca ataaagaaaa agatgttcag gattatagta ttaagcgggt
    10381 tagtgccaat gtaagattaa atgagcgcta tacttttgat gaatttgtag tgggtaaaaa
    10441 taacaatttt gcttatagtg cggcaaaagc ggtagcagaa tcgcctggtt atacctataa
    10501 tcctcttttt atttatggtg aaagcggaat gggaaagacc catctgatgc aggcaattgg
    10561 aaattatatt ttgaaagaag gacgaaactg ttccatttac tatataactt ccgaggcgtt
    10621 taccaatgaa atgattgatg gcattcgcag taataaaatg ccggaattca gagcaaaatt
    10681 cagaaaagtt gacctgcttt tgatggatga tattcatttt ctttcccgca aagaaggaac
    10741 gcaagaagaa tttttccata cctttaatgc cctgtttgaa aacaaaaaac agattgtgct
    10801 cacttcagat agacccccca tagatattcc cgatttggaa aaacgcttgg ttacacgctt
    10861 tgaaagcggt ctgctctgcg atctgaaaag tcctgatttt gaaacccgag ttgccatttt
    10921 aagaaaaaaa gcagaaccgg aaaatgtggt tttaagcgat gcaatattta attttattgc
    10981 cgataacatt accagcagtg tgcgtgctct ggaaggttcc ttaattcgta ttttggcatt
    11041 ttcatcctac aataaactta atcctgaaga catagatatt aatctggcag cggaaatttt
    11101 atccgatatg atttcggaaa aggtgcattc tataacttta gatgctataa cggaaaaggt
    11161 ctgtgaatgt tatggaatta cacctgtcca attactggaa aaaacccgca aaccgcaagt
    11221 tgcttttccc cgtcaggtag caatgtatct ggcaaattat ttaattcctc agctctcttt
    11281 aaaagatatc gcggaatact ataacaggaa agatcatact acggttctcc atgctaaaaa
    11341 gatggtggaa aaccagtttc gggaaaaaga ggacttccga attcagctgg aagaaataat
    11401 taaaaacctg aaaaaataat gaagatagaa cgcagataac tggatttatt ggattttcag
    11461 gatttgttat ttttattatt ttgtgtagac agaggacgct gttcttccgc aaaaaaatta
    11521 ttataagatg cagagctacg gcaagctatg gatacaataa aacaacaaga acatcgtttt
    11581 tataaaaaag ttatccacaa gtgtggatag aattaagtgg gtggaagtga ataagttata
    11641 tataaatgct tgcggtaact ttttctgtgg ataagtaagg ataaaaaacg aagttataca
    11701 caagttatcc acaaggaaat acttgaggga aaagcataca aggcaaagag aaaagaaaaa
    11761 gcttgaccaa atatccccct atccacaacg cctacttata atactataaa aaagatttaa
    11821 ttaaaatagg attattatta tggaattgct tccaaaggta ttaaaactgc tactctgcat
    11881 aattattctg gtaattatgc taagcggttg tactgataag aaaaacttaa ccggtgataa
    11941 tttcagcgat attagagcca taactataga agataaacct ggaattatta tgggtttcag
    12001 ttttcctgcc gaaaccaaag aagaaataac aggaaaagag gcaaaactgc ttgccggtaa
    12061 ctaccaaaac gctgttgcaa tatgtctttt aaggtttacg gagcttcctt ctccgataac
    12121 ggaaacggac agctgttttc taactataaa catcagtaag cgttccgctc ttccgcgtaa
    12181 tcctttaatc ttaaagctat acaaattaaa cagcgtctgg cttgattcct taaaccttaa
    12241 cgatatttcg gaagatgatt taagcttatt ggtgcaatac actgttgcag atacagtagg
    12301 cagttccgga aaagaaatta ccttcaatct ttcttcctct gaagttgtta actggagtaa
    12361 tcctgattct accggctgga atttcgtgct taaaacagaa aatgagggct gggtagaaat
    12421 aagttctctg gaaggaacta acaagccaac tcttaacttc aaatataaaa cggaaactgg
    12481 aggtgaatgg ataaattata ataaatctcc tgctaaagat acttatattt tagaagcacc
    12541 ggaaacaaat ccttccgtac tatggaaaat cagcaatctt aattcctccc gtctttatat
    12601 aaaatatgag ccaacttaca ctctctttaa agataacgaa ggtaacacat taacagatat
    12661 acaactaaaa cggctgaccg tgaataaagc ggaaatagtt ctacatataa aaaacaatga
    12721 ctattacaat tcctctacca gctattctct ttatcctttt aatgttgtaa aggactctat
    12781 agataccccc gttactttgg taaaaagcga ttatgaaaag cttgcttata tagattacag
    12841 cattggattt attaaaggtg attctttaac ggttgatgtt actccaataa ttcaggcata
    12901 tacttccgga gaaaagatac ctaaaggaat aatgattcaa tctactcagg aacgcagaaa
    12961 tttcggcaca ctggaatttt gggattgcaa tgaaagcact ccggctgaga aaaaaccgta
    13021 tatcaaaata acttacactc ctccctattt ataagatgaa aagatttgct ttaatacctc
    13081 taatccttat actgttattt gcctgcacta aaaaagaaga aaaaggcatt gctttggctc
    13141 aggtagataa agaaattctc tatctggatg actttaagtc cactttttcg gttgaggatt
    13201 gggaaaattt aagccccgaa ataaggaaaa aatacattga ggattgggta aacctcactc
    13261 ttttagctca ttatgccgat aaacagggct taaataaaga tcaagcagtg aaacaaaaaa
    13321 ttaactatgc aagcaaaaaa gtgaaagcca atgctttaat tgctaaacgc ctttccgaaa
    13381 ttgaggtctc ggaagatcag ctcttcaact attttcgtct acatcaggca gaatttcaaa
    13441 aaaatgcagt agaatataat atccggcgta tagctttaat ggataagatt tctgcggaaa
    13501 atgttctgca acaattaagc cagggaatga gtttcacgga agctgtgcaa cgctattcaa
    13561 cagaagaact taaaaacaga ggcggattta tgggtatggt ttctgccacc ggtcctgatt
    13621 ctcttttctg gttaaaagca cgcgaattga aagaaaatga ttatggcttg gtctataaag
    13681 aaccgatgtg gtatgttttt caaatagtgg aaagtaaaat tacacctcag gaagctaatt
    13741 ttgaagatta tcgggcagaa atcaaacgca aggttctgct ggaaaaacaa gaccaggtgt
    13801 atcaagaatt aattaaagaa atcaaatccc aaaccagtga gatttattat tactaataag
    13861 gaagttaaga atgcgaaaaa cagcaataat ccttatcctg ttagttctaa ctttatcttt
    13921 aagcgcagaa ctgatagata aaatagtagc caaagtagga accgacatta ttctgctttc
    13981 cgatttacag aaagaaatgg tgcagatgca aagtgcaggt ttattggaag aaaatacaga
    14041 ccccagagat gttctggatg aaatgataaa tcagaaattg attatccaga aagccaaaga
    14101 cctgaatatt acagttaaca acgatgaaat aaaagcaatg gcagagaact atctgaaaaa
    14161 agtaaaagct caatatccca gttcctcagc ttttgccgcc gaccttaaaa aaagtaagct
    14221 tacagaatcc gatttactgc aactttataa agatatacta actgaacaag cactttccga
    14281 ccagattttg aaaaaagaaa taattaacaa ggtctctgtt acggaagcag aagtgataaa
    14341 tttctataat gctaccaagg attccttagc cgtaaaaccc gtttcctggg atttaggaat
    14401 tattttcaga gaaataaaac ccagccagga aagcaaagag gcaaaactgg cagaaataaa
    14461 agaaattcaa agacgcctga aaaatggtga ggactttgct accctcgcca gcacggaaag
    14521 tgactgtccc agtaaagaag ttggaggtga tctcggtttc tttaaaagag gacaaatggt
    14581 aaaacctttt gaagatgctg cctttgcttt acagcttgga gaaataagcg acatcgtaga
    14641 aagtgaatat ggctatcaca ttatccgctt agaagaaaaa agaggtaatg aaatccgtgc
    14701 ccgccatatc ttaaaagccc ttactcctac agctgaagat tctttacgcg aaagacaact
    14761 gatggaagaa atccgtaatc gctatgccaa tggtgaaagc tttgcttctt tggcaaggga
    14821 atattctatg gatcaagaaa gcagagaaga tggtggctct ttaggagaat ttacagaaag
    14881 agaccttcct tccctttttg ctacccaaat tttacaaaca cctgtaggag aaatgactcc
    14941 cgttctggaa aatcagggta tgctttacat tttctgccgc ttgaaagaat accctccccg
    15001 tatttataaa tatgaagaag taaaagacca agctcgggag atggttctta aaagaaaaca
    15061 gattgatgcc tttaatgcct ggatagaaaa tttacggcgt gaggcgtatg tacaaataac
    15121 tttatgactg ctgaaaaacc ccaaaagctg aatatttata cctttttagc aacactttgc
    15181 ggaattggct ttattcctgt tatgccggga acttttggtt ctcttcttgc ttatggaatt
    15241 tatatgctct tttccggttc accttttcaa gggaaggcac tgttttatgc tttgcctgcc
    15301 ttaatagtgc tttgtttaat agctgttttt ttaagcacta aagcggaaaa aaccctggga
    15361 agagataacg gaagtattgt aatagatgaa ttctgcggtt attatgtaag cgttttgttt
    15421 ttacctcaca gttggctaat aggcctttat gcttttgccc ttttccgggt ttttgatata
    15481 gccaaaccct ttcctattaa aaaagttcag gaacttcccg gtggctgggg agttgttacg
    15541 gatgatatta ttgccggaat ttataccaat atacttattc aaatactaac taaaatatat
    15601 ccaaaattct ttggattata ggagaaaaaa tgaactacat attactacag gcagcccaaa
    15661 aaggcactac tcaacaaggt ggtgcaggca caactttgat ctttatgatc ataatgttcg
    15721 ttattctgta tctcttgctt atcagacccc aacaaaagaa agccaaggaa acccaaaaga
    15781 tgcaggattc actaaaagta aacgataagg tcataaccag ttccggaatc tatggtcgcg
    15841 tcgtttccat aaaacccgat aaaggaattg tggttttgga aattgatgat accaataaag
    15901 tgcggataga tattcaaaaa aacgccatca taggagttgt tacaacatct gaacccgaac
    15961 cagaaaaatg aaatataagc ccaaaatctt acctgtgcgt ctctacgggg atgatttttt
    16021 aagaaagaaa ctgccagaaa ttgattataa tactccaggt ctgcaagaat ttattgaaga
    16081 tttaatctac actatgtatg caagagatgg aataggcatt gctgctaatc aggttggctc
    16141 tttctaccga atgattgtta tagacccaga acaggataat aagttgaata aaaaaagtcc
    16201 aatagtgatg attaaccctg taattgaaaa cagagaaggc gaagttgttt acgaagaggg
    16261 ctgtatcagt ttacccgatg tctttgcaga tgtgtcacgc agtaaaaaaa ttacctattc
    16321 ctacacggat agaatgggaa atcgcataac ggaaaccgct gaagaaataa aagcagttgt
    16381 tattcaacac gaatttgacc atctggaagg tattttattt attgaccatt tgggaactct
    16441 tgaccgcttg aaaataatgc acaaacttaa aaaactgcag gcaagagcgg ttaacggaca
    16501 gaatatactg gaaggactta ctttataatg cgttacctgt ttattggttc ttcagattac
    16561 ggtttgccag cactgaataa attacggcaa aatggttatg aaccctcttt aataatcagc
    16621 cagccggcta aacctgcagg acgcaattta cacttaactc caactcctgt atcacaatat
    16681 gccattgaac aaaaattacc gctttttact cccgtagaca taaattcagc agaaagtata
    16741 gcaaaaatgg cagaacaaaa ggcagatata attgttactg ctgcctttgg tgaatttata
    16801 aataaaaaaa tccgtaatct ctgccctttt ggagctgtaa atctgcatcc ttcattactg
    16861 cccaaatata gaggtgccag tcctattcaa agtgccattt taaatggaga aaccgaaacc
    16921 ggaactacaa tatcccttgt ttccgcaaaa atggatgcag gacctattct ggctcagaca
    16981 aagctttcca tagctgaaaa tgaaacttac agcgaactga aagaacgcct agcggaacag
    17041 gcaggtgatt tactactcca gttttggaag aaacttataa cggaaaaatc actttgcgga
    17101 acgaagcagg atgaaagcaa ggcaacttac tgctataaaa taaccaataa aacctgccaa
    17161 attgactgga ataaaagaac tgaggaaatt cataaccaaa ttcgcgcttt ttctttaact
    17221 cccggtgcct ggactctgtt tagaaataat aaattgaaga tattgtgttc agaaaagaca
    17281 gaagagccgg caaatggtga accggggcta atttgcaaga tagagaaaaa gcgcggtttt
    17341 tttgttaact gtctggactt taaattgctt attactaaag ttcagccggc gggaaagaaa
    17401 gtaatggaag cagttgcttt tataaatgga gcccgcctaa gagaaaaaga aaaattagga
    17461 ggggaaaaat gaaaaaagaa tacttttcta taatcaaatt ccctgtgctt atcagctttt
    17521 ctttgcttat tataacgctc atcttcttct ttatcgtgtt aggcagttat caccgtttag
    17581 gtctttctgt ctggggaaat gtgctctata caatactctt tattgtgatt gtaattttaa
    17641 tcggcacttg gattttagct cttatcagca cccacagaaa aatcaagccc ctttggctga
    17701 gacattatct ttcctggatg ctggtgaata tctattatcc tttagcgaga gtttttggca
    17761 ggatttgttt tcaaaaaatg gacaccatac aggagtcatt tctctatttt aataatgaaa
    17821 ttgtagttac caactatcag ggaatgcatt cttccaatct gcttttactt ttgcctcatt
    17881 gcctgcaaag ttccaactgc aagattcgca taaataacag cattatagaa tgtcaggaat
    17941 gcggtggctg tgatatggct aaaatgaagg cattaaccaa ggactatcaa attaaaactg
    18001 cagttgccag tggtggttct ttggcaagaa aattggtaaa tgatactcat ccggatatta
    18061 taattgctgt tgcctgcaat cgggacttaa cagaaggagt tcgtgaaagc tggcgttacc
    18121 ctgtttatgc agttttgaat gaacgcccca atggtccgtg ttttgaaacc actgtaagtc
    18181 ctgcagtaat tgactttgcc atccataaat ttgttcaata aaggagagaa aacttgaaac
    18241 gcttcaatct cttcacaata atccttattc tattgcttaa tgcctttatt actatggctt
    18301 taatcaataa atacggaaat aaaactcctg aaaccgatac cttcgtttcg ccgggaacat
    18361 ctcaaccgag taaagcatct tctgctaaaa ggataaattc tattaccgaa gctgtaaaag
    18421 cagtagagcc ggcagttgtt agcatcaatg ttataaaaac agaatatgtg cgagccgtat
    18481 ctccttttgg tttcggtttt tttgatttct tcggttccat acctttactg cgtcaggtag
    18541 agtccattgg cagcggtgtt atttatgatc ctaaaggtta catcgttacc aatgcccatg
    18601 tagttagcgg agctactcaa attaaagtta ttttacctga taaacgcgaa ttttccggaa
    18661 tcgttgctgg aattgacgaa attcacgatg ttgccaaaat aaaaatcacc ggaaataatc
    18721 ttcctgttgc cgaaatgggc aattccaacg atttaattat cggagaatgg agtattgctt
    18781 taggtaatcc ttatggtttt ttaatgaatg attccaagcc cagtgtttcc gtaggagtga
    18841 tttctgctgt gaatcgtaat tttgctgccc gtcaagacag gcgtgaatat aaatctatga
    18901 ttcaaacgga cgctgctatc aatcctggaa acagtggtgg tccccttgtt aatatcaatg
    18961 gagaagtagt tggtattaat acctttatct tttccgaaag tggaggcaat atcggtatcg
    19021 gctttgctat tcctataaat tccgtaaaga ccattttagc aaagatatga acagaaaata
    19081 taccttattt ctgctgatat tgctttcctg cggtttgctt tttgcccaaa cagcactcag
    19141 ttgctatgat attcagtata atccagaggg gggaggcgat tcaccttatt tggatcaaat
    19201 tgttaccgtt caaggaattg taagtggcgt tacttattat tccggaagcg gttataacaa
    19261 ttacggtttc ttcattagtg atccaacagg gggtccctgg agcggattat ttatctataa
    19321 tcaaacctat catcccgctt taggagattt agtgcgcgta acgggaactg taaaagaata
    19381 ttatggttta acggaaattt ctcaggtatc ttcccttctg gttttaagcc agggcaatcc
    19441 tcttcctgcc cccactgaaa taaataccgg ttctctgaat aatttcagca gtggagaaca
    19501 atgggaaagc gttttagtga aggtagtaaa tgttactgta acagttgaac ccaatagtta
    19561 tcaggagttt tatgtagatg acggcagtgg gcaatgccag atagataatc aattctttgg
    19621 ctctaatcac agctggaatg atattacatt aggacaggtc tacagcgaaa taatcgggat
    19681 tgtggattat tcttacagct actatgccat caatccgcgt tcggaaacag atatgatttc
    19741 aggagataac acagtaactt tggctattcc tcatcagtca gtcggtttgc acagttccgt
    19801 ttccgttcct ataaatgctt atcgtataga gacagcgcag aattatcaat cctattcttt
    19861 cagcatttct ttcaatcccc ttattctgaa ttatgaatca atcaacactg caagcacttt
    19921 atcccaggca ggaaatgtaa ttgcttctgc ggaaagtgga gtgttatctg tttcttatca
    19981 aagcaattca attttaagcg ggcagggaat tcttttaaaa ctaaatttct acgctgccaa
    20041 tacaggaacg acttcgctaa ctttttcgga tgtaatattc ggagccgatg taattcaaag
    20101 ctgtatcaat ggaagtgtaa ctgtaaatag cagttataat tcacctggcg atactttaac
    20161 cgttatccag cgtcctcttc tcaatattcc ggaaatagtt gttcccggag aaacattaaa
    20221 aataacctgt ttagcccctc aaaatacaag caactggaat gcttggctga gacataacaa
    20281 taaacgcctg aatttaccgg ttacaaacag ccaatggtta actttaccaa accgttggga
    20341 acttacaaca acggttcctt ctgttcctgt ttatgaatta tacgatttgg aggtctctgc
    20401 cagtggaggt ataagcgata ttacagctaa tgccgttcag gttattccca gccgaaaaaa
    20461 caattactat tttgtccata taacagactc gcatatgccc aatcgtctgt attatcctaa
    20521 tgctggtttt gatgctgatt ctactgccgt agaagatttt agagccgtga tggacgacat
    20581 taatattatt catcccgagt ttgttctttt taccggcgat ttaattaacg aaggagagct
    20641 ggagggcttt gccaatcaat attggtttgg ctggacgcaa aaattgctca ccgaatttga
    20701 agttccggtt tatgttactg ccggaaatca tgatattggc ggttggaatc aaacccctcc
    20761 tcctgcagga agtgcccgaa aaaactggtg gaaatatttt ggttggagct ggctgaataa
    20821 tgaagacctg agctttccct atcatacaca ggattactat tttacctata ataatgctgt
    20881 ttttataggg ttagagagct atgacaatta tgattactgg cgaccagcta tttatggtga
    20941 aacaagctat actgatcagc aaattgcctg gttgaataat accctttctc tttttccgaa
    21001 ttacaaaaag gttcttttcc accattacga ttttaaggaa cagctttttc tttcctcgtt
    21061 gggaatagat ctgtctcttt ggggtcatat tcattccaat aacggttcaa tttatactta
    21121 tccctataat ttagctacgc gtagcgtttg tgatggcaac cgtgcttatc gggttatcag
    21181 agttaataat gaacaattga ctccttatgc cacaatttat gctggagcaa caggcaataa
    21241 cctttctgtt caattttatc cttccaatta tggagttgcg gatagtgtga aggcaattat
    21301 tatcaataat cagccccttg cttttgaaaa tgctcagctg aaatttatta tgcctcaacg
    21361 agatacgggt tataatgttt caggaggaac tcttacccag gtagataaaa gtggcaatta
    21421 taatatctgt tatgtgcgtg tttccctttc tgccaatagc acttccactg ttaccgtaac
    21481 agaaaacgga gtggaaatat ccgaccctgt tcaagttcct gttgtattca caataaaatc
    21541 cgtttatcct aatcctttgg taaagcaggg aaatctgcaa gttattagcg ataagacatt
    21601 tccggagctt cacctgcagc tttttaacct gagagggcaa ctgatttatc aggaatgcct
    21661 aaaaggaatt tctcaaggtg aaagtcatct ttcttttacc ttacccgatt ccttagccag
    21721 cggaatttac tttctgcgtt ttcgggaaga taaaacccaa attcacaaaa tcctcatttt
    21781 gcaaaggtaa tatcttatgc ctgagaaaag accccaaacc tcaaaactcc tctatctccc
    21841 gtggattatc aagcaccttt cctattactt aaaactgaat actcacatct tgaaaaaagg
    21901 tgttccctta aaggaaatac tgattctgaa atatcctttc tggttcaaag atgccaaaaa
    21961 tcctcctctg ctttccgtag atattaccga tgcctgcaat ttacgctgca tttattgtaa
    22021 taatcccttt ttcccttatc cgagaacaat gatgaatcag gatgttttta aggccttaat
    22081 tgagcgcctg aaacgccatc ctgtaagcag aatcagaatc agtggaggtg agccaaccct
    22141 gcatcctcaa tttgatacaa tgctgaaaga aatgtcctat tactgtaaat atttatctat
    22201 catcaccaat gggcaatggc aaaatcctga agtgaatgaa caactcctta attgcggact
    22261 tagctttatt gaactttcta tggatgccgg cggaaaagaa atctacgaaa aaagtcgtcc
    22321 tggtgccgat tacgaccttt tcttccaaaa cctgaaacag ctttataaac tgagaaatca
    22381 gtataaagtt gacctcatca tccgcattcg tttaatggtg cgtccttcaa ctatggataa
    22441 agaaaaggaa gatagccgtt ttttacgcaa attctgcgat tgtgttttac cacaacatat
    22501 tttacagcat cccgaaacac cttcccaaga agatgttttt atccaaaatt ctatcctgca
    22561 aaattcactt cctgtttgca ctgttcccta tagagacctc caaataagac ctgatggttt
    22621 ggttcctctt tgtccagcaa aaggatgcgc tatcaatcct gaagaccgta tttttattgg
    22681 agacattcaa aaagatgaaa ttcttgatct ctggaatgca cctttgctaa aacaaatgcg
    22741 ggatgcgcat cgcaacagaa agggagaaat attaaagctt tgtaggaatt gccattatag
    22801 ctgaaaagag ggttgagagg gtttgaaggt ttagagggtt ttgaaaatta aggatacaga
    22861 taggttgtca tcttcatcaa tccttcttaa aaaaggttga ttccttgaaa agcaaagagc
    22921 gaaataaatg ctttagaagg gtagataaac cactcccaaa aattatgctt caaaatttat
    22981 acttaaaaga aagaccgttt atttcgggtt gtgcattcag cattagacga ggtgtatttt
    23041 tcaggactaa tcttgctgtt atataaccga taacggcacc ggcaaaaaca tcgctgctcc
    23101 aatgctgatc atcattcaaa cgcgaaaggg aagttaaagt ggcaattgta taaacagttg
    23161 gaggcaccca gcgggaattt ttgtattgtt ctgctaaaac aggtgccaaa ctccaaacca
    23221 aagttgtatg tcccgaagga aaactatccc gggaaaaaga aattccgttg ccgttccaaa
    23281 attcttttcc ttcttcttta tagggttttt gcctttggga agccagcttc aaagtttggg
    23341 taaccgccaa agagaggagt gaacttttca ggcatagcag agaagtatct attgtctttt
    23401 catcttttgt taaatatcct cccaaggcag ttaccgccat taccggaaac ataattttta
    23461 cttctccgaa ttgtttggca accgtaaaaa tatcatccgt aacttcattt ctgttccgtt
    23521 gaaagcaatc ccgcaaatca tcatccagaa aatataaaca acctccggta ataactattg
    23581 cacttgcttg caaccaatta tccttttgcc aactaaaagg tgctgtagct ccggcataaa
    23641 ttgtttcagg ataggataaa aaataatctt gcaaaaagtt atcttcctct gcagaaaata
    23701 acggaaccgc cattagcacc aagaccacag caaaacagaa aagataaaat ttactcattt
    23761 gtctatcttc taaaattgcg gacaactgtc aagattaaac acaataatgt tcatagattg
    23821 atttatttca gccaccgaaa acaccgaaaa gaacatatct gaattattat cccttatctt
    23881 gaaaaaaagt tccagaggaa cgacagatac taacaacggg tttcaacccg gtgaaaagtg
    23941 caaaatgaaa catcttatct cttttgttaa gaatgagttc cgtaggaacg acagatacta
    24001 acaacgggtt ttaacccggt gaaaagtgaa aagtgaaaca tcttatctct tttgttaaaa
    24061 aaaagttccg taggaacgac agatactaac aacgggtttt aacccggtga aaaagaaccc
    24121 aggatattca tttcttcatc ttccgagaaa aagttccgta ggaacgacag atactaacaa
    24181 cgggttttaa cccggtgata agggcaaaat gaaacatctt atctcttttg ttaagaatga
    24241 gttccgtagg aacgacagat actaacaacg gg
//
LOCUS       DAOOZV010000027        24116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2239_length_24116_cov_63.645942, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000027 DAOOZV010000000
VERSION     DAOOZV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24116
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_24116_cov_63.645942"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..2066)
                     /gene="htpG"
                     /locus_tag="PLW08_04145"
     CDS             complement(69..2066)
                     /gene="htpG"
                     /locus_tag="PLW08_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPL51825.1"
                     /translation="MADKKTKEKGSLSIHTENIFPIIKKWLYSQHDIFLRELISNSVD
                     AIAKRKYADPNFKEEDMKVEVKLDKGKKTIEVMDTGIGMTAEEIKKYINQIAFSGAED
                     FINKYKDIQNNMIGHFGLGFYSAFMVADKVTIDSLSCTEGSEPAFWECDGSTEYIMSK
                     GKRKEIGTTVTVYLNEENAEYADPNKIRDILERYCNFMPYPIMFEGKQVNQKEALWNR
                     KPNEVKDEEYIEFYKNVFHDYMDPVFWIHLNVDFPFNLKGILYFPKLRNEPDFFKGEV
                     KLYCNNVFVADNLEDLIPEFLLLLKGGIDIPDIPLNVSRSFLQNDAQVKKISQYIVKK
                     VADHFNNTFKEDRKKYESYWEDINAFIKFGLLKDDDFFEAMKDIIIFKSASGDYVTIE
                     EYKARNKSEGERTKIWYAASEDTQVSYLNLMKEQGIEVVFQTSPLDTHLYQQLENKLN
                     NIEFVRIDSEVNDLLLNKEGVASDSEDKLKELFYKALDQKVEASFSKESYADFLKKFP
                     QAATLLSPYLIQKDEQTIIKPFELPENVRKELGAEAMKALEDNAFTEIKVEVKSLKSP
                     EIPSMIVFSEYMRRWHDMDLLAHQNPSSDMLKYHTLVVNRDNPIIKKILTFHEQGKDE
                     EVKTLCSYIHDLSLLEQKPFSGKELKSFIAKANQVLSYLSE"
     gene            2670..6590
                     /locus_tag="PLW08_04150"
     CDS             2670..6590
                     /locus_tag="PLW08_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL51826.1"
                     /translation="MKRIWIIILTLLLVFPLAGVIQETASLKHFLYGNEPNCAYDNWI
                     SHLAEGIAIQGYNTYAPYDRQTNGFGDFVVPNDDQLTAWNYIVGLFLAGYFDEAQTAI
                     NAVGFPYQVVLFNDTDSGITYRMLREVPNPEYYDDNGTDDTYDDENGAFAYGWGLYLY
                     NPLGSRPVIVTVPHPCDDFPTPAFALEAFQIWDAQFLLINGAGREVRWTNQGSYNNSK
                     SLSDPTRLYNHPFNVCYKMFADLIRTEFNIREFSPQIHSYDWNYHPGYPNVQISAGYN
                     RLCPNLPIRDLSSRKLDMINKGHHIMIPANTVGFHREVYLNDFYGVNYDLYPFLFDDG
                     EHCYEVNNYIDLPAYSQNHQMLYTQSGTTDYDVYDPFLHTEMDELPNSYELTENTYKW
                     FYGWNEALQCWNFDHLFDNFRMYYLRWVYDLESVMDEMFAMNDGLIPPTPVGFSIQNQ
                     SLNSITLNWQKVDCYDFDTFEILYSINPIDGSNYQIYNRNNNAILASPYCESVTVPGL
                     SSSNSYFFKIRSKDKNGNYSELSNQITTIPAPATIYTFTAHGLDNEVRLFWGVSGQTN
                     NLGYKVYRKAPTQTDYTLIDSYLTNPSLANTSVSNYTYWDYNVTNGQNWDYIISCTNV
                     NNQEFFYNYPVSAAVRPIHNLTLTNSTATLVDTIYFAQNPYASDSQDAYYDITKSNPS
                     GSSYVWSAFWEAYWGSNGTALSREVKGGYDTALDLKTWTIRIRSTEVNTPLYLSASDN
                     FSRAEKLYIYDSGNGTWHNLFSGPYQFMVPNNNVRTMTLYWGNLQPKISHINQNNQLF
                     QGGNNITFQWSAQNSFLIDHLDLYVKNESDSLFLTGNIPGNQNSWIYNIPPNVDMQKA
                     RFYINCYAVDGLIQTFVSPYTFALVPRMILHSNEAGWQTRSQIWPDLTPTVETLFGNG
                     AIALTPANDGTWQENDDLLFGIAYWINAPAVNFFNSTAEICPTEINSFPLQPGWNFIA
                     NPHYCSYPVQSLRFLVGTNPFLYSEMIAQNLVSRAVFVYREGKFQSVDTILPFEAFYI
                     KYYGDQSLNTYLRFYPYFEAPEIDPPDNFWQFKVNVSSAGSNADEFVLGTNPIATDGY
                     DFYLDLPSAPEKPFPGLSVFITREAPEDIFFRDKKLSAEFREAFSPINQQEKIWHFKL
                     VCNSTSPVEFNLSDIDLPNDYTVQFYLGSEYYNYEHTNSFTFFPNEPGTYTGLIKVAN
                     YPVSVSDLVQKPISNITVYPNPFNPTTTIAFNTFATQEVELSLYNLKGQKVRTLYQGI
                     LNGGEHKLIWDGKDNNGRTVSSGIYFAKIQAGKYTQIRKMMLIK"
     gene            6645..6788
                     /locus_tag="PLW08_04155"
     CDS             6645..6788
                     /locus_tag="PLW08_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51827.1"
                     /translation="MRKIMGWQISNAFISWKNACNAGFGKNLAEEKKAYFHIYNFTVI
                     YNV"
     gene            6804..7994
                     /locus_tag="PLW08_04160"
     CDS             6804..7994
                     /locus_tag="PLW08_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPL51828.1"
                     /translation="MQAGRIYLDNCVTTCPAPEVLTEMQPYWEKKFWFPGTFISTGES
                     TNSLINKAQNTIAETLEASAQEIHFTSGGTLANNIAIKGLANAYKQQGKHIIVSVVDY
                     PDLLTNAAYLEKNGFEVTYLSADSEGFISLEELKSAIRKDTILFMTTAVNHTVGTIQP
                     FAEIKAILESADHKIYYHLDAGQGYGKIPLKVNELGIDTMSISAHKIHGPQGIGALYQ
                     RTGTKLAQLIHGVKRVDNLQTGGLSIALIAGFAKAVELTFRDFEANTAYLRELSNYLL
                     DRIEKEIPYVELNGPRGEKRAPHNINVSIDFIEGEAITMMLDQKGITVATGSACASEG
                     LKANYVLMAMGKTHIQSHGSMKFTVSRYTTKAELDETVQALAEITQELRNRSPLYLAM
                     TKEK"
     gene            7996..8619
                     /locus_tag="PLW08_04165"
     CDS             7996..8619
                     /locus_tag="PLW08_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPL51829.1"
                     /translation="MQYSQKVLDHFMHPHNVGKMENPDAVATEGSPACGDQVTVYLKV
                     DDKTKTITDISFLSYGCASNIATASIITDMAKGKTLEEAKKITWRDAMDALDGLPPVK
                     VHCSVLAADTLQSAISNYEIAHGLKEVPNFGKATIEEELRKIIYPQIGEDIISLKMVK
                     YIGFQDGEVTIDMNIMKFDQWRDNIAEEIREHLQKYPEVKKITINFP"
     gene            8758..10731
                     /locus_tag="PLW08_04170"
     CDS             8758..10731
                     /locus_tag="PLW08_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPL51830.1"
                     /translation="MRKLVFLAFLFSTCLNATVITIGSENQLNKGLPIEPIAHFSYSQ
                     QIYLPSEIGCTGIITTLSFQYNVISNVFYNSNKSWKIWLGHTPIAEITNWIPLNDMQV
                     VYDNNLQESDFLGGLPGNGWLVIELETPFLYNGMDALLVAVDENSIGYGNNSDDFSCS
                     SCPLDYAIQYQSSQINPDPLAPPESGWNLKNFRSNLQITIEAQYYTPVQPIPANASNN
                     IPVDTTLSWTSICNSFSLVLGTHSDSLLQVVDQTPLTYWQPETPFQFGKTYYWQVTGY
                     ANNCEYVSPLWSFTTITEQISPPQNLTGCYNGSASQLNWNAPVTGNQHYYKIYRNASF
                     YITTLETSYYDYNVQQGLSYYYYVTAVSFSGSESTPSNVITITIPAGGTDRILFQGFE
                     TCPSFTGIIANWQNLDLDLAPTSFWQNVTYPNAGNPAGWLCFEPQSTIPPLTDHPAFE
                     GNKMLASICCIPPPNNDWLISPVIHLGTASSLQFKARSAYANFGLERLSVLISNINSE
                     PASFVPLHQNTYIAVPVEWTNYEYDLSAWNNQNVYLAWRCLSVDAMALFLDNIEVFSH
                     NGYVSNQDIELPKPLFCCYPNPSKDSFTLESKDGTAFDVELYNLKGQCLYKEKGIKKL
                     QSSKLNCNLANGIYFIRIAQAGKIVTLKQVVIK"
     assembly_gap    10743..10842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10973..11788)
                     /locus_tag="PLW08_04175"
     CDS             complement(10973..11788)
                     /locus_tag="PLW08_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51831.1"
                     /translation="MTKKVVSLLILIISLSLLSAENFIIGNGSGAQSTVPVDGHYNYG
                     WSKFFYTSSQLQTAGILNPVEITKIAFQIGGSMPLVNYLMDNQSIYIGPFPGTNYGSD
                     YLGHPGYENKTLIYNGSISFNGPGWFFITLSTPYTINPNSGVEILWENRDGSKISNPP
                     QFCFTNYSPYSKEVHLVSDLSFPAGDGTTTSKCPNICFSTGGLEAPQVEISQSGNSFI
                     LSWSLIPTASSYSIYVSDSPSGPWNFLTAITGNQYQLTATDPKKFYYIKAISR"
     gene            complement(12025..13002)
                     /locus_tag="PLW08_04180"
     CDS             complement(12025..13002)
                     /locus_tag="PLW08_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL51832.1"
                     /translation="MNILILRLSSMGDIILTQPICAILQKHFAGCQIDYVCKEEFKEL
                     PEMFAPPVHPLVYEKTLKFHLWLSKRKYDLILDLHNKFSTFLLMLFLRAPRKVHYSKK
                     RGLRRDIVKGNKKLKIDSTVELYASALKKLGINEPWSYPRLQVTELPNSAPAKHNNAL
                     SGEIKIAVFPGANHFTKRYPAANWIQLINSNPQYDFTLFGSQADEELCKFITPKCSTN
                     CRNKCSTLTLQELLTELIAYNLIISGDTGPMHLAATLNKPQIAIFGGTHPRLGFRPLN
                     DKAIILCAELPCQPCSLHGKEKCPLNHFNCMNAISPALLSSAIKKALEG"
     gene            complement(13003..15603)
                     /gene="alaS"
                     /locus_tag="PLW08_04185"
     CDS             complement(13003..15603)
                     /gene="alaS"
                     /locus_tag="PLW08_04185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPL51833.1"
                     /translation="MISSKEIRQQFIDFFLNKGHIFVPSSPVIPEDDPTLLFANAGMN
                     QFKSIFLGQKEITYKRVVNSQKCIRAGGKHNDLEEVGKDGYHHTFFEMLGNWSFGDYY
                     KKEAISWAWELLTEVWKIPKDKLYATVYKTDTEAFDIWKQETDIDPTHISYFDDKDNF
                     WEMGETGPCGPCSEIHIDRGISHCTRQNEPGHICKINGGCSRFIELWNLVFIQYNREA
                     DKTLSPLKNKFVDTGAGFERLAQVLQDKNSNYETDLFMPLINKIEELSGVDYTQETGM
                     PHRVIADHIRCLCFALADGGFPSNEGRGYVLRRILRRAARYGRLLGFAEPFLHLLVPL
                     VIEQMAHHFSELNGKEDYIKMVIKAEEERFNKTLDTGLEKFREITQKLKGEVISGSDA
                     FILYDTYGFPPDLTAILAEEKGLKIDYEGFEKEMQLQRERARKASKFTLFVNNEDWIE
                     FSPVTPTEFVGYKENTVQTYIQRYAIQEKGLILMQLAQTPFYAESGGQVSDTGKIYNE
                     EFELEITNVRKQDEYIIHYGKLKRGTVNKAPVTAEIDRERRKSIARNHTATHLLHKAL
                     REVLGEHTVQKGSLVQPDYLRFDFAHFRALTLEELRKVEDIVNQAVLDNRKVNTTVKN
                     IEEAKKEGAIALFGEKYSEKVRVVCVENFSQELCGGTHISSTGEIGLFKIISESSSAA
                     GIRRIEAITGISALKWVQDLQDKLSRISTLLNSPVKNIESKLEATLEQISALERELKS
                     AKAKEEEKFVQELLSKATKQENYLLIKAQTEFDNLEELKTTAEQLKTKMQGTIAVLFN
                     LYQDKLNILCVVSADLIPKYNAGKIVAKLANELKGKGGGRTDIAMAGGKDIANLASVM
                     EKVPEIIASI"
     gene            complement(15614..16546)
                     /gene="trxB"
                     /locus_tag="PLW08_04190"
     CDS             complement(15614..16546)
                     /gene="trxB"
                     /locus_tag="PLW08_04190"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPL51834.1"
                     /translation="MYDVIIIGAGPAGLSAAIYSARSGLKTVVLERGLIGGQINVTEI
                     VENYPGFAESISGYELTDRMHRQAEHFGAEFRDEEVTALGMEGLCKIIETTENKYRTK
                     AVIFCTGAYPRRLNVPGEEKFTGHGVSYCATCDGALYRDKIVAVIGGGDSAIEEGIFL
                     THFAQKVIVIHRRDELRAQKIIQERAFQNPKIEFVWNSVVQEIRGNTRVQELEVYNRK
                     TNSLSVIPVDGVFIYVGILPNNKLLESRIELDSAGFVITDDFMQTNVPGIYAAGDIRK
                     KVLRQVVTATSDGAIAGWSAEKWITENYSSPQTE"
     gene            complement(17116..19215)
                     /gene="recG"
                     /locus_tag="PLW08_04195"
     CDS             complement(17116..19215)
                     /gene="recG"
                     /locus_tag="PLW08_04195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPL51835.1"
                     /translation="MAESKKTTDLNSKVKYLKGVGEHRAQLLSKLGIKTILDLMEHFP
                     KVYISRKLSATLGDLKPGDMLAFTAVISWVDVHQTAKGRNILSIGVSDGKVGIICSWF
                     TYPPVYEKMFLPGRLVWLNGTITEFNGQLEMVHPEFELIDDWEDTKEDFWKNREVLPV
                     YPLTEGINQKLMRRLIYNAFALYAGVIEEKLPENIIAKHHFLDRKTALQKMHFGQNPE
                     EIEKVRQRFAYEDFFYTQLLWARHKTFHTTKTKGIKFINKKQLTTGVYKKLPFTLTKA
                     QKKVLWEIFGDMCSEKQMSRLLQGDVGSGKTVVTLFAMLLAVENGYQSALMAPTEILA
                     EQHYETITNLLKGFEVQVCLLKGGVYKGKEAIKKAIAEGSAQIVIGTHALLQKDINFK
                     RLGFACVDEQHRFGVEQRAKLANLAEHPDLLYLSATPIPRSLAMTVYGDLEVSILDEL
                     PPTRKPVRTIIRPSSKIDTVYSEVRQELALGRQVYIVCPLVEESEKIALLDATKLYEY
                     ISQKVFPEYPASLLHGRMPVKEKDMIMQKFKAGEIKILVSTTVIEVGVDVPNASVMIV
                     EHAERFGLAQLHQLRGRVGRGSAQAYCYLIEHSPVSEVAWQRLTTMTKTTDGFIIAEK
                     DLELRGPGEIFGYEQSGIPQFRFANLVRDQEILKLARQDAFEIVRADPDFELPENALL
                     KKIYFSQFTDKEKLILY"
     gene            19409..20452
                     /locus_tag="PLW08_04200"
     CDS             19409..20452
                     /locus_tag="PLW08_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51836.1"
                     /translation="MKKIQKRLLVIVLFLLVFLLAEGQNFAQFKEELLNTKDWNTARQ
                     KIIAYIPTTNNVEELRELQSIWESVEPNVCKQYFFNAAQKNPHSPVYQYLALRLEEDE
                     TLQMKGAEELCLNHPDFYWGYRLYIVDFMAWLLNAELEIPDPLNGQELALKIIDEGYK
                     RFPDDDYFHIFQFHRYRLTQNYPQAEKELKLTKDRNLLMANWMRIKYFLVQEKNATLY
                     SSYLPPLFSDLIKSGQMASADSIYIFAEGYVEILQETESWQSLEQFLAQNPVLLNSAP
                     YFDVYAGLLARKEDWNALGNELLSAYNKKVIDSEHLSQYLTKWEDNLCHQPHWTELKQ
                     KAETQSQLPSPQY"
     gene            20460..21101
                     /locus_tag="PLW08_04205"
     CDS             20460..21101
                     /locus_tag="PLW08_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPL51837.1"
                     /translation="MKNPLRNFLTPDEQKILLFLGLIILCGCFLDYFGWNTLQATPTD
                     LDTLRQVVKEDKPLQLDIRIATFEELLCLSGIGEKRAKDIIAYRETNPFTSVNQIMNI
                     KGIGAKTYAKILPDLLVFGDTTNFKLSPTPSSTSKTKSATPSKANNTSPVNINTANLE
                     ELCTLSGIGEVKAQAIIDWREENGSFETIEDIMKVKGIGAKTFEKNKDRLTVK"
     gene            complement(21208..22260)
                     /locus_tag="PLW08_04210"
     CDS             complement(21208..22260)
                     /locus_tag="PLW08_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPL51838.1"
                     /translation="MFKNKIKSLLLLLLFSVAFCLNADYYDSVINLTGDDLFYGLRNL
                     ISNNNNTSYDASKVVLYQTLDNFNGYVTCVYTGQEYYVGFDYTGSTNPNTEHTYAQSW
                     FNGTDTTIKKSDLHHLFITNSVVNSSRGNYPFDVVANHNTANVYYTYTPWQSYRGYNA
                     QNRMVFEPADEFKGNIARALLYFYTRYSGESLVQQNVDMLPTLLIWHSFDPPDAAEIT
                     RNMGVFNYQNNRNPYIDHPEFVTKIWGGNDVEDAVLPVVPDLTINAVYPNPFTSELNI
                     SITTQKSALLTTSVYNIKGQLIYRESSVLIAGENKVFWKGQDNKGQNTPAGIYLIKVQ
                     SADKQAVAKVLKVFEG"
     gene            complement(22273..22881)
                     /locus_tag="PLW08_04215"
     CDS             complement(22273..22881)
                     /locus_tag="PLW08_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPL51839.1"
                     /translation="MNSIIIETFQEAQELISQVLNRENILPQIEAMAKQMADVLLNKG
                     KLIACGNGGSMCDAIHFAEELSGRFNKDRQALPAIALSDPGYLSCVANDYGWDNVFAR
                     GVDAFALKGDLILGISTSGNSTNIIKALKTAKQKECFTASLLGKEGGKLKGFCDYEII
                     VPSNNTARIQEIHGIIIHLLIELIEKELFTSQGENPQSPLFC"
     gene            complement(22895..23974)
                     /gene="add"
                     /locus_tag="PLW08_04220"
     CDS             complement(22895..23974)
                     /gene="add"
                     /locus_tag="PLW08_04220"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPL51840.1"
                     /translation="MKIKMTKEFIKKLPKTDLHVHLDGSVRIETIIDLAKKYNIKLPT
                     MDPAELRKLLVCGEQTTSLDDYLRAFPIVNLVLQNEEGLRRAAYELAEDASAENVRYM
                     EVRYSPILHTDQGLKLTEISQAVIDGLKQGERDFGIKTGVIICGIRNMDPTTSLKLAE
                     LAVAFKNKGVIGFDLAGGEYKHPAKDHKEAFDLALHNNLNITIHAGEAYGPESIHQAL
                     HYCGTHRIGHGTRLVEDGDLLNYVNDHRIPLEICLTSNLHTKAVPDIRSHPIDFYIDY
                     GLRVTINTDNRTISNTTVTDEYMLAINELGLDYPTVKYIILNGFKSAFLPYKERVRLI
                     NQILKEIDEIEEQELKTRVKVKENL"
BASE COUNT         7192 a         4836 c         4343 g         7645 t
ORIGIN      
        1 agggactgca ttatacagtc cctgtagttt atactttcgg gattaaaaaa ccctgcaatt
       61 atttttattt attcgcttaa gtaactaagc acctgatttg ctttagcgat aaaactttta
      121 agttctttgc cgctgaaagg tttctgctcc aaaagcgaaa gatcgtgaat gtaggagcaa
      181 agggttttaa cttcttcatc cttaccctgt tcgtgaaagg taagaatctt tttgattatg
      241 ggattatccc tgtttaccac caaagtatgg tatttaagca tatcggaaga aggattttga
      301 tgagccagca gatccatatc atgccagcga cgcatatatt cactaaacac aatcattgaa
      361 ggtatttcgg ggctttttaa gctcttcact tctactttaa tttcggtaaa agcattatct
      421 tcaagggctt tcattgcttc agcacccagt tctttacgca cattttccgg cagttcaaag
      481 ggtttaatga ttgtttgctc atctttttga atcagataag gacttaagag ggttgcagct
      541 tgaggaaact ttttcagaaa atcggcataa ctttctttgc tgaaacttgc ctccactttt
      601 tggtctaatg ccttgtaaaa tagttctttc agcttatctt cagagtcact tgcaacccct
      661 tctttattta gcaagaggtc attaacttcg ctatcaatgc gcacaaattc aatgttattc
      721 agcttatttt ccagctgttg ataaagatgg gtatccagag gactggtttg aaaaacaact
      781 tcaataccct gttctttcat taaattcagg tagctgactt gggtatcttc actggctgca
      841 taccaaatct ttgtcctttc accttcgctt ttattgcggg ctttgtattc ttcaatagtt
      901 acatagtcgc cgctggcgga tttgaagata atgatatcct tcattgcctc aaagaaatcg
      961 tcatctttca gcaaaccgaa tttgataaag gcattgatat cttcccagta gctttcatat
     1021 ttcttgcggt cttccttgaa ggtattattg aaatggtcag ccactttttt tactatatac
     1081 tggctgattt ttttcacctg ggcatcgttt tgtaaaaaac tgcgggagac attgagagga
     1141 atatccggaa tatcaattcc gcctttcaag agcagaagaa attccggaat caggtcttcc
     1201 aaattgtccg ctacaaaaac attattgcaa tagagtttca cttctccttt gaagaaatcg
     1261 ggctcattgc gcaatttggg aaaatataaa atccccttca gattgaaagg aaaatccaca
     1321 ttcagatgaa tccagaacac agggtccata taatcgtgaa agacattttt gtaaaattca
     1381 atatattctt catctttcac ttcgttgggt ttacggttcc aaagtgcttc tttctggttt
     1441 acctgtttgc cttcaaacat aattggatag ggcataaagt tacagtatct ttccagaata
     1501 tctcttattt tattgggatc agcatattcc gcattttctt cattcaggta aacagttacg
     1561 gtagttccaa tttctttgcg ctttccttta ctcataatgt attccgtact gccgtcacat
     1621 tcccagaaag cgggttcact tccttccgtg caagaaagcg aatctatagt tactttatcg
     1681 gcaaccataa aagcagaata aaatcccagt ccaaaatggc caatcatatt gttctggatg
     1741 tccttgtatt tattaataaa atcctcggca cccgaaaagg caatctgatt gatgtatttc
     1801 tttatttcct cagctgtcat tccaattccg gtatccatta cttctattgt ttttttgcct
     1861 ttatccagct taacttctac tttcatatcc tcttctttga aattaggatc ggcatatttg
     1921 cgtttggcta tggcatctac cgaattggaa atcaattctc ttaggaatat atcgtgctga
     1981 gaatagagcc atttcttgat aatcgggaag atattctcgg tatggatgct caaacttcct
     2041 ttttcttttg ttttcttgtc tgccattatg ttttaatctc cttattttaa ttcattgtgt
     2101 ttctggagat aaaataatcg gtcttggctt attgtcaaga ttttttagca gtcctataac
     2161 ttgattgcta aatttgtgca aaacaaatca ggactcgtta tgcggagggt tgatatcctc
     2221 atcaaccacc aaaatcggaa gagttaaatg actcccggtt tgcagtttgc ctgcctattt
     2281 ttcccggaca aaaccttctt ataaccctgt aacaaataag taacaatccc gtaacgagat
     2341 tactgcagtg ttagggaagt gttacggaag tgttacgggg tcttcttgga attaactgat
     2401 ttgcaggaag ttacaaattt gtagataaat accctaaaga acataaattt atcacgcaga
     2461 atgcgctgat tatgctgatt ttatttgtcg tgccgtcagt agttataagc acccaaaaaa
     2521 gatatgttca ggaataaatt tgtgaagacg ctaataactc ctgacttaca agggatattg
     2581 ttcttcctgt atattattct tgactaaatt tgaaagaact caattaatgt tttttataag
     2641 ttcttagacc ataaaaattg ggagtattga tgaaaaggat ttggataata atactaaccc
     2701 tcctgctggt ttttcctctg gcaggagtaa ttcaagaaac cgcttcgcta aagcattttc
     2761 tttatggaaa tgaacccaat tgcgcttacg acaattggat ttcccattta gctgaaggta
     2821 ttgcaattca gggctacaat acttatgctc cttacgatag acaaaccaac ggttttggtg
     2881 atttcgtagt tcctaatgat gatcaattaa ctgcttggaa ttatattgtg ggcttatttt
     2941 tagccggcta ctttgatgaa gcgcagacag cgattaatgc tgtcggattt ccttatcagg
     3001 ttgttctttt taatgatacg gacagtggca taacttatag aatgttgcgt gaagttccca
     3061 atccggaata ttatgatgat aacggaactg atgatactta tgatgatgaa aacggtgctt
     3121 ttgcctacgg ttggggtttg tatctttaca atcctttagg gagtagacct gttattgtaa
     3181 ctgttcctca tccttgtgat gattttccta ctcctgcttt tgctttagag gcatttcaaa
     3241 tttgggatgc ccaattttta ttgatcaatg gagcaggtag agaagtaaga tggacaaatc
     3301 aaggtagtta taataattcc aaatccttat ctgatcctac gcgcttatac aatcatcctt
     3361 tcaatgtctg ttataaaatg tttgcagacc ttattagaac cgagtttaat atccgggaat
     3421 tttctcctca aatacatagt tacgattgga attatcatcc gggttatcct aatgtccaaa
     3481 tttcagcagg atacaatcgt ctctgtccca accttcctat tagagatcta tccagccgaa
     3541 aactggatat gattaataag ggtcaccata taatgattcc agctaatacg gtaggttttc
     3601 acagagaagt ttatctgaat gatttttatg gtgttaatta tgatctctat ccgtttcttt
     3661 ttgatgatgg tgaacactgt tatgaggtaa ataactatat tgatttgcct gcctattccc
     3721 aaaatcatca aatgctgtat actcaatccg gaacaacaga ttatgatgtt tatgatccct
     3781 ttctccatac agagatggat gaattgccca attcttatga attaaccgaa aatacctata
     3841 aatggtttta cggctggaat gaagcattgc aatgctggaa ttttgaccat ctttttgata
     3901 actttaggat gtattatctg cgttgggttt acgatttgga aagcgttatg gacgaaatgt
     3961 ttgcaatgaa tgatggctta attcctccta ctccagtagg tttttccatt caaaatcaat
     4021 cccttaattc catcacctta aattggcaaa aggtagattg ttacgatttt gatacctttg
     4081 aaatactata ttccataaat cctattgatg gttctaatta tcagatttac aatcggaaca
     4141 ataatgcaat tttagcttca ccctattgtg aaagtgttac ggttccagga ttgagttctt
     4201 ctaatagcta tttcttcaaa atccgctcaa aagataaaaa cggaaattat tcggaacttt
     4261 ccaatcagat aactacaatt cccgctccag ccactattta tacttttact gctcacggtt
     4321 tggataatga agtccgcctt ttttggggtg tttcaggaca aactaataac ctgggatata
     4381 aggtctatag gaaggcacca acccaaactg actacacttt aattgattct taccttacca
     4441 atccctctct ggcaaataca tctgtatcta actataccta ttgggattat aatgttacta
     4501 acggtcagaa ctgggattat ataatcagct gcacgaatgt aaataaccag gaatttttct
     4561 ataattatcc tgtttctgca gctgttaggc ctattcataa tttaactctt actaactcta
     4621 ctgccacttt ggtagatact atttactttg ctcaaaatcc ttatgcttcc gatagccagg
     4681 atgcctatta tgacataacc aagtccaatc cttccggttc aagttatgta tggtctgctt
     4741 tttgggaagc atattggggt tccaatggaa cagcactttc ccgggaagtt aaaggtggct
     4801 atgatactgc tttagacctg aaaacctgga cgattagaat tcgctctacg gaagtgaata
     4861 cacctctcta tttatctgct tccgataatt tcagccgggc ggaaaaactg tatatttacg
     4921 attcgggaaa cggaacttgg cataacttat ttagcggacc ttatcaattt atggtcccca
     4981 ataacaatgt aagaactatg actttgtatt ggggtaacct gcaacccaaa atttctcata
     5041 ttaaccaaaa taaccagctt tttcaaggtg gcaataatat taccttccag tggagcgcgc
     5101 aaaacagttt tctgattgac catctggatt tgtatgttaa aaatgaaagt gacagcttgt
     5161 ttttaacagg aaatattccg ggaaaccaaa acagctggat atacaatatt ccccccaatg
     5221 tagatatgca aaaagcccgt ttttatatca attgctatgc tgttgatggt ttaatccaaa
     5281 catttgtatc gccatatact tttgctcttg ttcccaggat gattttacac tctaatgaag
     5341 ctggctggca aacgcgctcc caaatttggc ccgatttaac tcctacggta gaaactcttt
     5401 tcggtaatgg agccattgct ttaactccag ctaatgatgg aacttggcag gaaaatgatg
     5461 atctcctgtt tggtattgcc tattggataa atgcaccggc ggtaaatttc tttaattcca
     5521 cggctgaaat ttgtccaact gaaattaact cttttccgct gcaaccgggt tggaatttta
     5581 tagccaatcc tcattattgc agttatccgg ttcagagttt acgctttctg gtaggaacaa
     5641 atccttttct ctacagtgaa atgatagctc aaaaccttgt ttcccgagcc gtatttgtct
     5701 atcgggaggg aaagtttcag tcagtagaca caattttacc ctttgaggca ttttatatca
     5761 aatattatgg tgaccagtcc ttaaatactt atcttcgttt ctatccctat tttgaagcac
     5821 cggaaattga cccacctgat aatttctggc aatttaaagt aaatgtttct tctgccggta
     5881 gcaatgctga tgaattcgtt ttgggaacaa atccaatcgc caccgatggt tatgattttt
     5941 acctggattt accttctgct cccgagaaac catttcccgg cttgagtgtt tttataacaa
     6001 gagaagcacc cgaagatatc tttttcaggg ataaaaaact ttcagctgaa tttagagaag
     6061 cattttctcc aataaaccag caggaaaaaa tctggcactt caaacttgtt tgtaattcca
     6121 cctctcctgt ggaatttaat cttagcgata tagacctgcc caatgattat accgttcaat
     6181 tttatcttgg ttcagaatac tataactatg agcatacaaa tagctttacc ttctttccta
     6241 atgaacctgg aacctatacg ggattgatca aggttgcaaa ctatcctgtt agcgtaagtg
     6301 acttggttca gaaaccaatt tccaatataa ccgtttatcc caatcccttt aatccgacaa
     6361 ctactattgc ctttaatact tttgcaactc aggaagttga actctcctta tataacctta
     6421 aaggacaaaa agttcgcacc ctttaccagg gaatattaaa cggtggagaa cataaattga
     6481 tttgggatgg aaaagataac aacggcagaa ctgtaagcag cggaatttat tttgccaaaa
     6541 tacaggcagg aaagtatact caaattcgga aaatgatgtt gattaaataa gagctggggg
     6601 tttatgcata gaccattttg aggggataaa ccggaaaaat taagatgcgg aaaataatgg
     6661 gttggcaaat ttccaacgct tttatcagct ggaaaaatgc ctgtaacgct ggttttggga
     6721 aaaatttagc agaagaaaag aaggcatact ttcatatata taattttact gtaatctata
     6781 atgtttgaaa aggagaaaaa ataatgcaag cgggaagaat atatcttgat aattgcgtaa
     6841 ctacctgtcc tgcaccggaa gttttaacag aaatgcaacc ctattgggaa aaaaagtttt
     6901 ggtttccggg aacttttatc agcacgggtg aaagcactaa tagcttgata aataaagctc
     6961 agaatacaat agctgaaact cttgaagcca gcgctcagga aatccatttc acttctggag
     7021 gcactttggc taataacata gctatcaagg gattagctaa tgcctataaa cagcagggaa
     7081 aacatataat cgtttccgtt gttgattatc ctgatttatt aactaatgct gcctatctgg
     7141 aaaagaacgg ttttgaagta acttaccttt ctgccgatag cgagggcttt atttctctgg
     7201 aagagctgaa aagtgctatc cgtaaagata caattctgtt tatgactact gccgtaaatc
     7261 atacggtagg aacaattcag cccttcgcag aaataaaagc tattttagag agtgccgacc
     7321 ataaaattta ttaccacctg gatgcaggtc agggctatgg aaaaattcct ttgaaggtaa
     7381 atgaactggg aatagacaca atgtctatca gtgcccataa aatacacgga cctcaaggta
     7441 ttggtgcact ctatcaaaga acgggcacaa agctggcaca attgattcat ggagttaaaa
     7501 gagtggataa tctgcaaaca ggcggtttaa gtattgctct tattgccggt tttgctaaag
     7561 ccgtggaact tacttttcgt gattttgaag ctaatacagc ttacttaaga gaactttcca
     7621 attacctttt ggacagaata gaaaaggaaa taccttatgt tgaattaaac ggaccccgag
     7681 gagaaaaaag agcccctcac aatatcaatg tttccattga cttcattgaa ggtgaagcaa
     7741 ttacgatgat gcttgatcaa aaaggaatta ctgttgccac gggaagtgcc tgcgcctcgg
     7801 aaggattaaa ggcaaattat gttttaatgg caatgggaaa aacacatatt cagtcacacg
     7861 gttcaatgaa atttaccgtt tctcgttata caactaaagc cgaactggat gaaacagttc
     7921 aggcattagc agaaataaca caagaattgc gcaatcgcag tcctctttat ttagcaatga
     7981 ccaaggagaa ataaaatgca atactcacaa aaggttttag accactttat gcatccccat
     8041 aatgtaggta aaatggaaaa tcctgatgct gtagccacag aaggaagtcc tgcctgtgga
     8101 gatcaggtaa ccgtttatct gaaggttgat gacaaaacaa agaccattac agatatcagc
     8161 tttctttctt atggctgcgc ttccaatatt gctactgctt ccataattac cgatatggca
     8221 aaaggaaaga ccttggaaga agcaaaaaaa attacctggc gcgatgctat ggatgctttg
     8281 gatggacttc ctcccgttaa agttcactgc agtgttttag ctgctgatac tttacaatcg
     8341 gcaatttcca attacgaaat agcacatggc ttgaaagaag ttcccaattt cggtaaagca
     8401 actatagaag aggaactgag aaaaattatc tatccgcaaa taggagaaga tattatctct
     8461 ctaaaaatgg tgaagtatat tggctttcag gatggagaag tgactattga tatgaacata
     8521 atgaaatttg atcaatggcg ggataatatt gcggaagaaa tccgtgaaca cttgcaaaaa
     8581 taccccgaag tgaagaaaat tacaataaac ttcccgtaga aaaagctaac caggttaaca
     8641 ggttaaggga aaaagtgaaa gggtaaaacg caggaataaa atagtgagtt ttctgataga
     8701 aagagtagag aatgatagta caggttaaaa tccaataact tatgaaggaa agatgatatg
     8761 cgtaaacttg tatttttagc ttttttgttt tctacttgtt taaatgcaac cgttattact
     8821 atcggaagtg agaatcaact gaataaaggt ttacctattg agccgattgc tcatttcagc
     8881 tattcacagc aaatttatct gccttcggaa ataggttgta ccggtataat aactaccctt
     8941 agctttcaat acaatgtcat cagtaatgtg ttttataatt ccaataagag ctggaaaata
     9001 tggctgggac atactcccat agctgaaata acaaactgga ttccacttaa cgatatgcaa
     9061 gttgtttatg ataacaatct tcaagaatcg gatttcttag gtggattacc aggaaacgga
     9121 tggttggtta ttgaattaga aacacctttt ttatacaatg gaatggatgc tttattagta
     9181 gccgttgatg aaaacagcat tggctatggc aataattcag atgattttag ttgtagttcc
     9241 tgtcccttgg attatgctat tcaatatcaa agttcccaaa tcaatcctga tccattagct
     9301 cctccagaaa gcggctggaa tttgaaaaac tttcgcagta acctccaaat cacaattgaa
     9361 gcacaatatt acactcccgt tcagccaatt cctgcaaatg catcaaacaa tattcccgta
     9421 gatacaactt tatcctggac aagcatctgt aacagctttt ctcttgtttt gggaacgcat
     9481 tccgattcct tactgcaagt ggtagatcag acacctttaa cctattggca accggaaaca
     9541 ccttttcaat tcggtaagac ctattattgg caggtaaccg gttatgctaa caattgtgaa
     9601 tatgtttctc ccctctggag ttttactact attacggaac aaatatctcc cccacagaat
     9661 ttaaccggtt gctataatgg ttctgcctct caattaaatt ggaacgcacc tgtaaccgga
     9721 aatcaacatt attataaaat ataccggaat gccagttttt acataacaac ccttgaaaca
     9781 agttattacg attacaatgt gcaacagggc ttaagttatt attactatgt aacggcagta
     9841 agtttttcgg gctcggaaag cactccttcc aatgtaataa caatcactat tccagctgga
     9901 ggaacagaca gaatattatt ccagggcttt gagacctgtc cttcttttac tggaataata
     9961 gcaaactggc aaaatctgga cctggatttg gctccaactt ccttttggca aaatgtaact
    10021 taccccaatg caggaaatcc agctggttgg ctttgttttg aaccccaaag tacaattcct
    10081 cctttaacgg atcatccggc ttttgaaggt aataaaatgc ttgcctcaat ttgttgtatt
    10141 ccacctccta ataacgattg gttgatttca cccgttattc atttgggaac agcttcttcc
    10201 ctacaattta aagcccggtc tgcttatgcc aattttggat tggaacgctt aagtgttctg
    10261 atttccaata tcaatagcga acctgcttct tttgtacctt tacaccaaaa tacctatatc
    10321 gctgttcctg ttgagtggac aaattacgaa tacgatttat ctgcatggaa taatcaaaat
    10381 gtttatcttg cctggcgttg tctttccgta gatgcaatgg ctttgttttt ggataacatt
    10441 gaggtcttta gtcataatgg ctatgtttcc aatcaggata tagaacttcc caaacccctt
    10501 ttttgctgtt atcccaatcc ctcaaaggac tcttttacat tagagagcaa agatggaact
    10561 gcctttgatg tggaacttta taacctgaag ggacaatgcc tgtataaaga aaagggcatc
    10621 aaaaaactgc aaagctctaa actaaattgc aatttagcta atggcattta ttttattcgg
    10681 atcgctcaag caggaaagat agttactctc aagcaggttg taataaaata atctttctcc
    10741 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagcagga aagatagtta
    10861 ctctcaagca ggttgtaata aaataatctt tctcctggtt ttatctgcca atagctttta
    10921 tactcagatg aacgacatcc tgtcgttccc taacaaaatc tttctcctga ttttatctgc
    10981 taatagcttt tatataatag aatttcttag ggtctgtggc agtcagctga tattggttcc
    11041 cggtaatagc agtaaggaaa ttccagggac ccgaaggcga atccgaaaca tatatagaat
    11101 aagaagatgc cgtaggtatc aaagaccaag ataagataaa ggaatttccg ctctgagaaa
    11161 tttctacttg aggagcttct aaaccacctg ttgaaaaaca gatattgggg cattttgaag
    11221 tggttgttcc atctcctgca ggaaaagata gatcagatac caaatgaact tctttactat
    11281 aaggactata atttgtgaaa cagaactgag gtggattgga aattttactt ccatcccggt
    11341 tttcccacaa aatttccact cctgaattcg ggttaatagt gtagggtgtt gatagagtaa
    11401 taaagaacca accgggaccg ttgaaactga tagaaccgtt ataaattaag gttttatttt
    11461 cgtatcccgg atgtcccaga tagtcactgc cataatttgt tcccggaaat ggtcctatgt
    11521 aaatgctttg attgtccata agataattaa ccagaggcat ggaaccacca atttggaacg
    11581 ctattttagt tatctccaca gggttcaaaa taccggcagt ttgcagttgg gaagaggtat
    11641 aaaaaaactt gctccaacca taattatagt gtccgtctac aggaactgta gattgtgcac
    11701 cagaaccatt tccgataata aagttctctg ctgagagaag agaaagcgaa attattaaga
    11761 ttagtagaga aacaaccttt tttgtcatta ttaacctctt ttgctgttta ttttaatagt
    11821 atatatcagc tctatattta tcctctattt tccagatgct ataaatatta tttacttttc
    11881 ctttgtgaaa taatatacag atttcgtcaa gacctttttt ctttatcttc acgcgtcctc
    11941 tctaccatat caatttgtgg gaagaaaaaa gttatttcag tagtgacgaa tcctgagtga
    12001 cgaatcctaa ctttttagag aatatcaacc ttccagggct tttttaatag cagaactaag
    12061 caaagcggga ctaatagcat tcatacaatt gaaatgatta agagggcatt tttcttttcc
    12121 gtgcaaagag caaggttggc aaggcagttc agcgcataaa ataatagctt tatcgttcaa
    12181 aggtcggaag cccaagcgcg gatgtgttcc tccaaaaatg gctatttggg gtttattcaa
    12241 agttgcggct aagtgcatag gtccggtatc accgctaata attaagttgt aagcaatcag
    12301 ttccgttaaa agttcctgca gagtaagcgt agagcattta tttctgcaat tagtggagca
    12361 ttttggggtt atgaatttac atagctcttc atctgcctgg gaaccaaaaa gagtaaaatc
    12421 gtattgagga ttggagttta tcagttgaat ccagtttgcc gcaggatacc ttttggtaaa
    12481 atgattggca cccggaaaaa cggcaatttt tatttcgccg gagagggcat tgttgtgttt
    12541 tgcaggagcg gaattcggta gctcagttac ctgcaaacga ggataagacc agggttcatt
    12601 tattcccagt tttttaagag cagaggcata aagctcaacc gtactgtcta ttttcagctt
    12661 tttattccct ttcacaatgt cgcgtctaag cccgcgtttt ttgctgtaat ggacttttcg
    12721 gggagctctt aaaaaaagca tcagcaaaaa agtagagaac ttattgtgca aatccagaat
    12781 taggtcatac tttcttttgc ttagccataa atgaaatttc agcgtctttt cataaacaag
    12841 aggatgaaca ggaggagcaa acatttccgg aagttccttg aattcctctt tgcaaacata
    12901 gtcaatttgg caaccggcaa agtgtttttg taagatagca caaatcggtt gagtgagtat
    12961 aatatcaccc attgaagaga gtcgcagaat aagaatattc atttagatag aagcaattat
    13021 ttccggcacc ttttccatta cagaagcaag attagctata tcttttccgc cagccatagc
    13081 aatatcagtt ctgccaccac cttttccttt taattcattc gcgagtttag ccacaatctt
    13141 tccggcatta tacttgggaa taaggtctgc acttaccaca cacaggatat tcagtttgtc
    13201 ctgatagaga ttgaacagga cggcaatcgt accctgcatt ttggttttta gctgttcagc
    13261 ggttgtttta agttcttcca aattgtcaaa ttcagtttgt gccttaatca acaaataatt
    13321 ttcctgtttc gttgccttag agagcagttc ttgcacaaat ttttcctctt ctttggcttt
    13381 agcggatttt agttctcttt ccaaagcgga aatttgttcc aaagttgctt ccaatttgct
    13441 ttcaatgttt ttaacaggag aattcagcag tgtagaaata cggcttagtt tatcctgcaa
    13501 atcctgaacc cattttaagg cagaaatacc tgttattgcc tcaattcttc taattcctgc
    13561 tgcggaagag ctttcggaaa taattttaaa taagccgatt tcacctgtag aagatatatg
    13621 agttccgcca caaagttctt gggaaaaatt ctcaacgcac acaacacgaa ctttttcact
    13681 gtatttttct ccgaaaagag caatggcacc ttcttttttg gcttcttcta tatttttaac
    13741 cgtagtattc actttcctgt tatccaaaac tgcttgatta acaatatctt ccacctttct
    13801 cagctcttcc aaggttaaag ctctgaaatg agcaaaatca aaacgcagat aatccggttg
    13861 cactaaagag cccttttgaa cggtatgttc tcctaaaacc tctctgagtg ccttatgcaa
    13921 aaggtgagta gccgtatgat ttcgggctat gcttttgcgt ctttccctat ctatttctgc
    13981 tgtaacgggt gccttattta cagtgcctct tttcagtttt ccgtaatgaa tgatatactc
    14041 atcttgttta cggacattgg ttatttccag ttcaaattcc tcattatata tttttcctgt
    14101 atccgatacc tgtccaccgg attcagcata gaaaggtgtt tgggcaagtt gcataaggat
    14161 taaacccttt tcttgaatgg catagcgttg gatatatgtc tgcaccgtat tttctttgta
    14221 accgacaaat tcagtaggag ttacaggaga gaactcaatc caatcctcat tattaacaaa
    14281 aagagtaaat ttacttgctt tcctggcgcg ttctctttgt aattgcatct ccttttcaaa
    14341 gccctcatag tcaatcttca agcccttttc ttccgctaag atagcggtta aatcgggcgg
    14401 aaaaccgtaa gtatcatata aaataaatgc atcggaaccg gaaataactt ctccttttag
    14461 cttttgggtt atctctctaa atttttccag accggtatca agggtcttat taaagcgttc
    14521 ttcttccgct ttaatcacca tttttatata gtcctcttta ccgtttagtt cactgaaatg
    14581 atgtgccatt tgctcaatta caagcggcac taacaaatgc aaaaagggtt cagcaaaacc
    14641 gagcaaacga ccataccggg cagctcttct taaaattctg cgtaaaacat aaccccttcc
    14701 ttcgttggaa ggaaaaccac catctgccaa agcaaaacag agacagcgga tgtgatcggc
    14761 aataactcta tgcggcattc ctgtttcctg agtatagtca accccagaaa gttcctcaat
    14821 tttatttatc agaggcataa aaaggtctgt ttcatagtta ctgtttttat cctgtaaaac
    14881 ctgtgccagg cgttcaaaac ctgctcctgt atccacaaat ttgtttttga gcggagaaag
    14941 tgttttatct gcctcgcggt tatattgaat aaaaaccaaa ttccacaatt ctataaagcg
    15001 cgaacaaccg ccatttattt tacagatatg tcctggctca ttttgcctcg tgcaatgcga
    15061 aatacctcta tctatatgaa tttccgaaca gggaccgcag ggtcctgttt cccccatttc
    15121 ccaaaaatta tctttatcat caaagtagct gatatgagtt ggatcaatat ctgtctcctg
    15181 tttccagatg tcaaatgcct cggtatcagt tttataaacg gtagcataga gtttatcttt
    15241 cggaattttc cagacctcgg tcagcagttc ccaagcccag gaaattgcct cttttttata
    15301 gtaatcaccg aaactccaat taccgagcat ttcaaaaaag gtgtgatgat agccgtcttt
    15361 tccaacttct tccaggtcgt tatgttttcc tccagcacga atacattttt gactattaac
    15421 cacgcgtttg tatgttattt ctttctgccc caggaagata cttttgaatt gattcattcc
    15481 agcattggca aaaagcaggg taggatcgtc ttccggaatt acaggtgagg aaggaacaaa
    15541 aatatgaccc ttgttaagga aaaaatcaat gaattgttgt cgtatttctt tactgcttat
    15601 cactttaatt cctttattcc gtttgtgggg aagaataatt ttccgttatc catttttcag
    15661 cgctccaacc ggctatagct ccgtcggaag tggcggttac aacttggcgt aaaacctttt
    15721 tccggatgtc acccgcagca taaattcccg gaacattggt ctgcataaaa tcgtcggtta
    15781 tcacaaaacc agcgctatcc agttcaatac gggattccag caatttgtta ttaggcaaaa
    15841 tacctacata aataaagacg ccgtcaacgg gaataacgct taaagaattg gttttccgat
    15901 tgtagacctc taattcttgc actcttgtat tcccacgaat ttcctgcaca acactgttcc
    15961 agacaaattc tattttagga ttttgaaaag cacgctcttg aatgattttt tgagctctaa
    16021 gttcgtcacg ccggtgaata acaattactt tttgggcaaa atgagttaag aaaattcctt
    16081 cttctatggc agaatcacct ccgccaatta cggctacaat tttatctcta tataatgctc
    16141 catcgcaggt tgcacagtag gaaaccccat gaccggtaaa tttttcttcc ccgggaacat
    16201 ttaagcgccg cggataagct cccgtgcaaa aaatcactgc cttggtacga tatttgtttt
    16261 ccgtggtttc tattatttta caaagtcctt ccattcccag agctgttact tcttcatccc
    16321 taaattccgc accaaaatgt tctgcttgac ggtgcattct gtccgtcagt tcataaccgc
    16381 ttatagattc agcaaaacca ggataatttt ctactatttc cgtaacattt atctgaccgc
    16441 caatcagtcc tctttccaaa acaacagttt ttaaaccgct acgcgcactg taaatggcag
    16501 cacttaagcc ggcaggtccg gctccaataa taattacatc atacattatg ttctatcctt
    16561 tagctgtttt gaataatagt agctaaaaca ctaaggcggg ttctttcgtc aaggaaaatt
    16621 gaattgctac gcctaatttt cttttatcgt aaatgatctc tgtatatttt atcctctctc
    16681 atagccatta tgtttccgat atgattccca tatcgttccc atatcgcgat atgggaatca
    16741 taagggagtc attttttcag cagacaagtt ttaagcataa acagggttcg ctgaaactca
    16801 gcttttatac taagaatcat ttacagcttt acaattttgt tccctacaat aactttcgca
    16861 gtgtatgcta cttgtaacac tctgacacat tttctcttac agattacact aacatcacta
    16921 acttataatt aataattctt ctcttaaata taaaaatctt attctattta aagtgtaata
    16981 taagttctgt atgtttcatt catcatttta tagctggcac gaatcctaaa tgctaaaata
    17041 attagtaggt agtataaaat ttatttcggc agtagcaaat ccttgcttta agctgcagct
    17101 acgccaaggt atttactaat agaggataag cttttcttta tcagtgaatt gggagaagta
    17161 tatttttttc aacagggcat tttccggcag ttcaaaatcc ggatccgcac gcacaatttc
    17221 aaaggcatcc tgcctggcta attttaatat ttcctggtct cgcactaaat tagcaaaacg
    17281 aaattgaggt attccggatt gttcataacc gaaaatttca cccggaccgc gcagttccag
    17341 gtctttttcg gcaataataa agccatccgt tgttttagtc atagtggtta aacgctgcca
    17401 ggcaacttcg ctaaccggcg aatgttcaat taaataacag tatgcctgag cacttcctct
    17461 gccaactcta ccccgtaatt gatgtaattg tgccaaacca aaacgctctg catgttctac
    17521 aatcatcaca ctggcattgg gaacatccac gcctacttca ataacagtag tagaaaccag
    17581 aatttttatt tctcctgcct taaatttttg cataatcata tccttttctt taaccggcat
    17641 tcttccatgc aaaagggagg caggatattc cggaaaaacc ttttgggaaa tatattcata
    17701 gagcttagta gcatccagaa gagctatttt ttccgattct tccactaagg gacatacaat
    17761 ataaacctgg cgtcccaacg caagttcttg gcgtacttca ctataaacag tgtcaatttt
    17821 gctggaggga cgaataattg ttctcacagg ttttcgcgtg ggaggcagtt catctaaaat
    17881 gctaacttcc aaatctccat aaacagtcat tgctaaactg cggggaattg gtgtggcaga
    17941 aagataaagt aaatcgggat gttcagccaa gttagccaat tttgcccttt gttcaactcc
    18001 gaaacgatgc tgttcatcaa cgcaagcaaa acccaatcgc ttaaaattta tatccttttg
    18061 taacagggca tgagttccga taacaatttg agcacttccc tcggcaattg cttttttaat
    18121 tgcctctttt cctttataaa cgccaccttt taagagacaa acctgcactt caaaaccctt
    18181 tagtaaatta gtgatggtct cataatgctg ttctgccaaa atttccgtag gagccatcag
    18241 cgcggactga taaccgtttt ccaccgctaa aagcattgca aacaaggtta caactgtttt
    18301 tccgctacct acatctccct gcagtaaacg actcatctgc ttttcggaac acatatcgcc
    18361 aaaaatttcc cacagcactt ttttctgtgc tttggtaagc gtaaaaggta actttttata
    18421 gacgccggta gttaactgct ttttattgat gaattttata cctttggttt tggtggtatg
    18481 aaaggtctta tgcctcgccc ataaaagctg ggtgtaaaaa aaatcttcgt aggcaaaacg
    18541 ctgacgcact ttttctattt cttcgggatt ctgaccaaaa tgcatctttt ggagagcggt
    18601 ttttctatcc agaaaatgat gtttggcaat aatattttcc ggcagttttt cttctatgac
    18661 accggcataa agagcaaaag cattgtaaat aagccgacgc ataagctttt gattgatgcc
    18721 ttccgttaaa ggataaaccg gtaaaacctc gcgattcttc caaaaatcct ccttcgtatc
    18781 ttcccaatca tcaatcagtt caaattccgg atgcaccatt tccagctgac cgttaaattc
    18841 cgttatcgtg ccattcagcc aaactaaacg acccggcaaa aacatctttt cataaacagg
    18901 gggataagta aaccaggaac atattatacc tacttttcca tcacttaccc ctatgcttaa
    18961 aatattcctg ccttttgccg tttgatgaac atctacccaa gaaattactg ctgtaaatgc
    19021 taacatatct cccggcttca aatcccctaa agtggcactt aacttgcgac tgatatagac
    19081 cttgggaaaa tgctccatca aatccaatat ggtctttatc cccagcttgc ttaaaagctg
    19141 tgcacgatgt tctccaaccc ctttcaggta tttaactttg ctattcaaat ccgtggtttt
    19201 tttactttcc gccatattgg aagccagctt agcaaacaac gcaaaattgt caattgcaga
    19261 aaactgttac aacctaacac tttaactttt ataaaaaact tatcctgtaa ccaaccaaac
    19321 tgctaaactt tgtttctttt gcttgacaga ttttatgagg taggcaataa agtaaaaaag
    19381 ctaaaatagg catttaagaa cggaaaatat gaaaaagata caaaaacgct tattggtgat
    19441 tgttttgttc ctgcttgttt ttttgctggc tgaaggacaa aactttgctc aatttaaaga
    19501 ggaattgctt aataccaagg attggaatac tgccaggcaa aaaattattg cctatattcc
    19561 tactaccaac aatgtagaag aacttagaga actgcaaagt atttgggaaa gtgtggaacc
    19621 gaatgtttgt aagcaatatt tctttaatgc agctcaaaaa aatcctcatt ctcctgttta
    19681 tcaatatctt gctttacggc tggaagaaga tgaaacactc caaatgaaag gtgctgaaga
    19741 actttgccta aatcatcctg atttttattg gggctaccgt ctttacattg tggactttat
    19801 ggcttggtta ctgaatgcgg aactggaaat tccagacccg ctaaacggtc aggaacttgc
    19861 tttaaaaatc attgatgagg gctataaacg ctttcctgat gatgattatt ttcatatttt
    19921 tcagtttcat cgctatcgct taacacaaaa ttatcctcag gcagaaaagg aactgaaact
    19981 gactaaagac cgcaatttat tgatggctaa ctggatgcga ataaaatatt tcctggtgca
    20041 ggagaaaaat gctactctgt atagttcata tctgccccct cttttttcgg acttgattaa
    20101 aagcggtcaa atggcttcag ctgatagtat ttacattttt gccgagggct atgtggaaat
    20161 tttgcaggaa acagaaagtt ggcaaagttt agagcaattt ttggctcaaa atcctgtttt
    20221 gctgaattca gctccctatt ttgatgttta cgcgggtctt ttagcccgca aagaggactg
    20281 gaatgctctt ggcaatgagc ttctttctgc ttataacaag aaagtgatag atagtgaaca
    20341 tttaagccaa tatcttacaa aatgggaaga taacctttgc catcaacccc attggacaga
    20401 actgaaacaa aaagcagaaa cccaatctca gcttccttct cctcaatact gatatgaaaa
    20461 tgaaaaatcc cttgcgcaat tttctcactc ctgatgagca aaagatattg ctttttttag
    20521 ggctaataat tctgtgtggt tgttttctgg actattttgg ctggaatact ttgcaggcaa
    20581 cccctacaga tttagatacc ttaaggcagg tggttaaaga agataaaccc ttgcaactgg
    20641 atattcgcat tgccactttt gaagaattgc tctgcctttc cggaattgga gaaaaaagag
    20701 caaaagatat tattgcctat agagaaacaa atcccttcac ctcggtaaac cagattatga
    20761 atattaaagg catcggagcc aagacctacg ctaaaatttt gcccgattta ctcgtctttg
    20821 gagatactac aaatttcaag ctttctccaa ccccttcttc cacttctaaa actaaatccg
    20881 caaccccttc caaagcaaat aatacttccc cagtcaatat taatacagct aatctggaag
    20941 aactatgcac cctttctgga attggagaag tgaaagccca ggcaattatt gactggcgtg
    21001 aggaaaatgg cagctttgaa accattgaag atataatgaa agtgaaggga attggagcca
    21061 agacctttga gaaaaacaaa gaccgcttaa ctgtgaagta aagaaataaa gaatgtgtga
    21121 ggtgtcctga aaatagcgga ctaaaattaa agagaaaaaa tccagttaat ccaattgaac
    21181 cagttatctg ggttctattt taaaatctca accctcaaat acttttaaaa cctttgcaac
    21241 tgcttgttta tctgcactct gcacttttat caaataaatt ccggcaggag tattttgtcc
    21301 tttattatcc tgtcctttcc aaaagacctt attttctccg gcaattaata cactgctttc
    21361 gcggtaaatc aattgaccct taatattata gacagaagtt gtcagcaaag cggatttttg
    21421 agtagtgata ctaatattca gttcagaagt aaaaggattg ggataaactg cattgattgt
    21481 caaatcggga acgacaggca aaacagcatc ttcaacatca ttcccgcccc agattttagt
    21541 tacaaattcc ggatgatcaa tataaggatt gcggttattt tgataattaa aaacacccat
    21601 attacgagta atttctgctg catccggtgg atcaaagcta tgccagataa gcaatgtagg
    21661 caacatatcc acattttgct gaactaaact ttctccgctg taacgcgtgt aaaaatagag
    21721 taacgctctg gcaatattac ccttaaattc atctgcgggc tcaaaaacca ttctgttttg
    21781 agcattataa ccccgataac tttgccacgg tgtgtaagta taataaacat tggcagtatt
    21841 atgattggca actacatcaa aagggtaatt tccccgggaa ctgtttacca ccgaattagt
    21901 aataaacaga tgatggaggt ctgatttctt tatagtagtg tcagttccgt tgaaccagct
    21961 ttgagcataa gtatgttccg tattgggatt ggtactaccg gtataatcaa agcccacata
    22021 atattcctgt ccggtataaa cgcaggttac atagccgtta aaattatcca gggtttgata
    22081 gagaactacc ttggaagcat cgtaactggt attattatta ttggaaatca agttccgcaa
    22141 gccataaaat aggtcatcac cggttaaatt aataacgcta tcataataat ccgcattcag
    22201 gcaaaaagcg acactgaata acagtaatag cagtaaagat tttatcttat tcttaaacat
    22261 ttttccctta ttttagcaga ataatgggga ttggggattt tctccctgac tggtaaacag
    22321 ctctttttct atcaattcaa tcagcagatg gataataatt ccgtgaattt cctgaatgcg
    22381 agctgtgtta ttagaaggaa caatgatttc ataatcacaa aatcctttca gtttgccccc
    22441 ctcttttccc aaaagagaag cagtgaaaca ttccttctgc ttagcggttt ttagggcttt
    22501 aataatattg gtggaattac cgctggtaga aattcctaaa atcaaatccc cttttaaggc
    22561 aaaggcatcc actcctctgg caaaaacatt atcccagcca taatcattag ctacgcagct
    22621 caaatagcca ggatcgctta aagcaatagc aggaagagct tggcggtctt tattaaaacg
    22681 ccctgaaagt tcttccgcaa aatgaatggc atcacacatt gaaccgccat ttccgcaggc
    22741 aatcagtttt cccttattta gcaaaacatc agccatttgt tttgccattg cctcaatttg
    22801 gggaagaatg ttttccctgt tcagcacttg ggaaatcaat tcttgtgctt cctggaaggt
    22861 ctctataatg atgctgttca agtttagttg ctccttacag gttttctttc actttcaccc
    22921 tcgttttcaa ttcctgttcc tcaatttcat caatttcttt cagaatctga ttgatcaaac
    22981 gcactctttc cttatagggc agaaaggcac ttttgaaacc gtttaagatg atatatttca
    23041 cggtaggata atccaaaccg agttcattga tcgcaagcat atattcatcg gtaacggttg
    23101 tattacttat cgttctatta tcggtattga tcgttacccg caagccataa tcaatataaa
    23161 aatcaatagg atgactgcga atatcgggaa ccgctttagt atgaagattg ctggttaaac
    23221 aaatctctaa aggaatcctg tggtcattta cataatttaa caggtctcca tcttccacca
    23281 accgcgttcc atgtccaatt ctatgcgttc cgcaataatg caatgcctga tgaatacttt
    23341 ccggtccgta tgcctcacct gcatggatag taatattcag gttattatgc aaagccagat
    23401 caaatgcctc tttatggtct ttggcaggat gtttgtattc ccctccagct aggtcaaaac
    23461 cgataactcc tttattttta aaggcaactg ccaattcggc aagtttcagt gaggtagtag
    23521 gatccatatt cctaatcccg caaataataa ccccggtttt aatgccaaaa tccctttcgc
    23581 cctgttttaa gccatcaata accgcctgcg aaatttccgt cagtttcaat ccctgatcgg
    23641 tatgcaaaat aggagaataa cgcacttcca tataacgcac attttctgca gaggcatctt
    23701 cagccagttc ataagcggct cttcttaatc cttcttcatt ttgcaaaacg agatttacga
    23761 taggaaaagc gcgcaaataa tcatccaaac ttgttgtctg ttctccgcaa accaaaagtt
    23821 tgcgtaattc agcagggtcc attgtcggta gcttaatatt atattttttt gccagatcaa
    23881 ttatggtttc aatccggaca ctgccatcca aatgcacatg caaatctgtc ttgggcagtt
    23941 ttttaatgaa ttccttagtc atcttgattt tcattttttc ttcctctttc ttctttaaca
    24001 gacataatat ctaataatgg ttctatgtca aattatttat atattttcta tctggttggt
    24061 tgttttgaaa tgcagtttgg caacttcgcc caagcgtttt tcaccgtatt gtttaa
//
LOCUS       DAOOZV010000028        23292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2344_length_23292_cov_62.449972, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000028 DAOOZV010000000
VERSION     DAOOZV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23292
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_23292_cov_62.449972"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..790)
                     /locus_tag="PLW08_04225"
     CDS             complement(62..790)
                     /locus_tag="PLW08_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPL51841.1"
                     /translation="MGCALGSFFNVLIYRIPKKQSIIFPGSHCENCKRPIPFYQNIPI
                     ISYILLKGKCSKCGAKIHWHHIVVEIITPLLFLALFFRYGLHDFRFYKYILLFSFLIP
                     IFFIDALHQIIPNVLSIPLLITGIIFVFIPGNDVGIINGLVTGAVVFCLLLFLAWAYQ
                     KIRGTEGLGGGDIWLFTALATYFGLTGIPYIFLLSALLGIIYFLIFIRDKSQPFAFGT
                     FIALAAVFWSVIGEEIVFQLINFI"
     gene            complement(834..1277)
                     /locus_tag="PLW08_04230"
     CDS             complement(834..1277)
                     /locus_tag="PLW08_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51842.1"
                     /translation="MRKVLLITMLLVMLLPLIAQSDIPHPNFSPYTNNGSSFLGLNKL
                     SMSHSMGFQAGTSSTGEGYYLSLYTNHLKYKFNPKLEMNLDLNFVNYGGFDTSSKFEL
                     DNANYSRVIPEFSLRYTPRDNIQIQLQMISGSILNPYKNNYLQNW"
     gene            complement(1338..2552)
                     /locus_tag="PLW08_04235"
     CDS             complement(1338..2552)
                     /locus_tag="PLW08_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPL51843.1"
                     /translation="MITNYFSETTKTMKSSLIRELVATTKNVPDLISFAGGFPSPATF
                     PTEILSKLFQEVVFQQGRDVLQYGNSEGDDQLKEEILKWENYPNLNRNNLIITVGATN
                     AIYYMSRTLLNVGDVVLCEAPSFLGSIVAFEAVGAEVHCVSMDNEGIDLNRLAEKVKR
                     LRDADKKIKFLYTIPDFQNPTGITMSYKRRYELLKYCEAEDILIMEDNPYSRLNFSEE
                     ELPTLLRIAQTELNNPYLVTEIVSFSKILGPGMRVAYAIGAPELINKMVSWQQKVTIS
                     PDCTTERVVAKFLAEGYMEPHIKNLCKFYKPYLDKMLACLSAEMPVYVQWTKPKGGIF
                     IWLWLPEDINADTLFYKAKDHLVTFIPGSKFYPEGQERFNCMRLNYTYSTMEQIETGI
                     KRLGELIKSIYV"
     gene            complement(2645..3940)
                     /gene="asnS"
                     /locus_tag="PLW08_04240"
     CDS             complement(2645..3940)
                     /gene="asnS"
                     /locus_tag="PLW08_04240"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPL51844.1"
                     /translation="MQDVFVKDIEKYLGQEVRLKGWVRNIRHSGKLLFIILRDGTGEI
                     QTVAFQPDLGEEAFETAKRLTLESSVIICGIPKLHPKQEGVYELQVTQVQPLQIADEY
                     PIGKKEHGPDFLLSNRHLWIRSQKQWAILRIRHTVYYAICNYLNEHNFIRFDSPILTP
                     NACEGTTTLFELDYFDEGKAYLSQSGQLYLETGIMSFGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAFVEHDENMQIQEDLIRYVIRTVLSECALELQILERNTEALKAADAPF
                     KRMTHYEAVELLRQKGSDITHDKDLGAQDEVLLTEDSEVPVFVERWPKEIKAFYMKRD
                     PLNPNLVLGDDLIAPEGFGELIGGSQREDDYELLLSRMQAENMPIDDYQWFLDLRKYG
                     SVPHSGFGVGLERLVAWLSGTPHIRETIPFPRMIYRIYP"
     gene            complement(3947..4948)
                     /locus_tag="PLW08_04245"
     CDS             complement(3947..4948)
                     /locus_tag="PLW08_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPL51845.1"
                     /translation="MIKIGVVGVGHLGQHHAQKFQNIDGAELVGIYDINEQRAKEIAQ
                     KLNTRSFNSYEELLTACDAIDISATTTAHYELAKTALLAGKHIFVEKPITSELAQAND
                     LLKLASERNLIIQAGHIERFNPVILKIEKEINNPLFIESQRISTFQPRGTDVPVVLDL
                     MIHDIDLILSFVHSPVTDIKASGVGIFTSTIDIANARIEFANGAVANVTSSRVSTKQE
                     RKLRFFQNDAYIRMDFLAKNVRVIKKSSDINSLLPQILMGAPNINPEQLLDIKSYDAD
                     DINKDALTLELESFIDCLKNNKKPVVDGEAGTRALEIATKIINQIQTQNTRINLLHR"
     gene            complement(4945..5898)
                     /locus_tag="PLW08_04250"
     CDS             complement(4945..5898)
                     /locus_tag="PLW08_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPL51846.1"
                     /translation="MKNILVLGAAGQIGSELVPYLRKLYGSNAVVAAYRSTPLTMEIR
                     EGGPCENLDAMEADKIFEVVKKYSIDTIINLVAVLSAKGEANPLKAWNINMGSLINSL
                     EIMREVKGAVFTPSSIGAFGPSTPLDNTPQDTIMRPTTIYGISKVAAELWSDYYFLKY
                     GVDTRGVRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKNYTCYLKPGTYLDMMYMPDA
                     MRACVELMEADPKKLKHRNCFNVTAMSIEPEMIAKEIRKHIPEFTIDYQIDPVRQSIA
                     ETWPNSMDQTAAMEEWNWKPEYDLPAMTLDMLKVLSQKLKK"
     gene            complement(6147..7310)
                     /gene="ygeW"
                     /locus_tag="PLW08_04255"
     CDS             complement(6147..7310)
                     /gene="ygeW"
                     /locus_tag="PLW08_04255"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPL51847.1"
                     /translation="MEKINTLINELETLKPDLFGKDFLLTWDNSLDSLKAVMLVAEIL
                     QCLHWQKKPWRIFDYGLAISIFRDNSTRTRFSFASAVNGLGLALAELDEVKSQIAHGE
                     TVRETANMISFLTEVIGIRDDMYPGEGHTYMLEVANAISEGYQQGILAQRPTVINLQC
                     DLDHPTQVLSDLLKLKDYFGGWENLKGKKIAMSWAYSPSYGKPLSVPQGIINLMTRFG
                     MNVVLAHPEGYDLLPETIQSAFSFAQESGGSFSQTTLMEEAFQNADIVYPKSWAPMSV
                     MQQRTKLLKAGDKQGLKELELHCLAQNEKYIDWECDDEMMKLTKNGNALYEHCLPADI
                     SDVSCENGEVAKSVFEKYRLHTYQEAGYKPFVIAAMIFMSKVKEPAAKLRSFV"
     assembly_gap    7354..7453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7454..7900)
                     /locus_tag="PLW08_04260"
     CDS             complement(<7454..7900)
                     /locus_tag="PLW08_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPL51848.1"
                     /translation="MKITVNNIEYTVSENLAELKLSAYLRENLDLTGTKIGCDIGVCG
                     SCTILVNNKPMRSCLLKLKDVADKQVLTIEGLASSDGTLHPVQQAFIDAGAIQCGFCT
                     PGMVLTAYAFLLENKQPTREEAREAIKGNICRCTGYQQIIDAILLAA"
     gene            complement(7897..10164)
                     /locus_tag="PLW08_04265"
     CDS             complement(7897..10164)
                     /locus_tag="PLW08_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPL51849.1"
                     /translation="MMFTDAFRNDAAAKVTGRAKYTDDYKLPDMLYAVPLHSQVASAI
                     IKSIDYSEALKQKGVVAVYTAKDIPGKIKFGQIIKDCPTIINRRIRSAGDVIALVVAE
                     TREIALSALPLIKVELEEIPAVYNPEEAMQETSPLVNPDFGSNICNHHKVRTGDIEQG
                     EKEADIIMEQEFNTSRVEHTYLEPETALCYLRPDGVMEVLGSMQHPFSTRRFVASTLG
                     CKLSEVEVKNIPMGGGFGGKDDTAALVCARAALCAYLTKRPVKLTYSREMSMRESYKR
                     HPYKLQYRMGLKKNGKIVFIKARIIADGGAYCSVTPWVTWRSTVQSCGCYEVPNVQSD
                     VYGVYTNNIFSGAFRGFGSPQVNFAIEQLIEMAAQKLNMDETEFRKINMVHQGSKTIT
                     DQKLDNHTVSLQEVMDSVLKEIDYENKVKKCSFGNPKIDEWYGIGYAISYRGVSLGAE
                     GTDMNSAIIYIQPDGSVLLETGVHENGQGAQSAMILIASEELGIPKELIRYRLPSTSN
                     IPDGGSTVASRGTIMGGGAVVNAAKIIKQRLCEGVEKALGIKDCSYKDGNVYFAQGEK
                     VADYFEAVRLCYDHQIYPYAFGVFQAPPISWDEETGHGNAYFSWVYSCQAAELKVNSK
                     TGKITLLNLVAAHDIGRAINPALLAGQIYGGMAMGAGYALYENFPLVNGEPKASNFNS
                     YRVMRSTDLPEMTAIFVENPDPNSPSKAKGIGEPALEITAPAIANALYRATGKRFTDL
                     PMAPQVWEYVKGKQK"
     gene            complement(10151..11248)
                     /locus_tag="PLW08_04270"
     CDS             complement(10151..11248)
                     /locus_tag="PLW08_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HPL51850.1"
                     /translation="MKASGIYDFHVHIGETIGGHLLADSWKSFNLLYEHNGLEGIGVF
                     VTESQSEPLSSKLGRMRKVSRSFLGKVFWHLTPRQLDIRRLENILKEDTDLKLYTTYR
                     EVGLYQSYESIEHLMEEMPSPKKRLLVHCEDDQIISEYSERYPFHNPSDHCLRRPEKA
                     EISAVEKLLDLSIKHHYPLHIVHISSPQSALLVQQAKKEADYITCETAPHYLLLNEEV
                     LNEPEGHRWLCTPPLRSESSRGLLLELLQDGVFDIIASDHCAFSSETKDVGILYPAKT
                     PMGIAGSGVLFTLLAEHLVEKGKLSMEQLFNFISFNPAKLMGFSVQNDKLSYERLGAP
                     IPVIPSWANTPNPWIDFWSYYELRRHHNDVY"
     gene            complement(11245..12426)
                     /locus_tag="PLW08_04275"
     CDS             complement(11245..12426)
                     /locus_tag="PLW08_04275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPL51851.1"
                     /translation="MKSYLTKYRCIECGKEFDPNELHYLCDECSRDYLPGMPLKGVLE
                     AVFNYEEIGRKWKQNPDPLLFSAVNPKFYPPLPVGNTPFFRVNVPGLKSGETASSDYT
                     KLWIKFDGLNPSGSYKDRASQLVVADALQKGIKEIVTASTGNAACSLACVGASAGLKV
                     VIFAPQNAPPAKLIQIQVHNAELHKIDGSYDDAFKAALEYTKTHNCMCRNTGYHPLTI
                     DGKKSAGIEIYVQNGYKVPDWIVIPVGDGVILTGIYKAFLDLKKAGITEKMPHLLCVQ
                     AESSDAITSYWETGIYHDAENPITIADSIKVKTPALAHWSVKALNETDGKCIRVSDKE
                     IQEAQLELAKETGVFGEPSSSATLAGLKKVVNHSWLKDAKDIVLLITGHGLKDPGAVK
                     L"
     gene            complement(12569..13768)
                     /locus_tag="PLW08_04280"
     CDS             complement(12569..13768)
                     /locus_tag="PLW08_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPL51852.1"
                     /translation="MYREIFARAKHYEDTSTRCLMDMVKIPSFSTKEKEVAECILKEM
                     QEIGMDDAYIDPLGNVIGRLGFGKKVIAFDAHIDTVYPGDLSLWDFDPFAAHIKDGKI
                     WGRGTVDQKSGMASMLTSARIMKDLDLGKEFSIYFTGTVMEEDCDGLCWQYILQEGEI
                     KPELVVITEPTNLNIYRGHRGRMEMEVSIKGLSCHGSAPERGDNAIYKISRIALEIEK
                     LHQNLRYDDFLGKGSICVTQVFFTGPSQCAVPDSARIHLDRRLTFGENKESAVAEVQD
                     ACKKAGYPDATIEILTYQEAAYTGLVYPTEKYYPTWVTPLDSFYVQNAADAYTKTLGT
                     KPLIDKWTFSTNGVSIAGMKGIPCIGLGPGNEIYAHSPNEACPIEHLTKAAAFYAALV
                     YRFSQEK"
     gene            complement(13937..15265)
                     /gene="ssnA"
                     /locus_tag="PLW08_04285"
     CDS             complement(13937..15265)
                     /gene="ssnA"
                     /locus_tag="PLW08_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HPL51853.1"
                     /translation="MNRYILKGGTILTFDSSHPVLEQAEILIDDGYILQIGSEKSFSD
                     EVCERIDVEGKIIMPGLINAHHHFYSTFVTGLGKAAPAKNFNAVLENLWWRLDKKLLS
                     EDNYYSALISILTSIRKGTTTIIDHHASPFAVKGSLSQIAKAVQETGIKASLCYEVSD
                     RDGDKIAQEGIEENYNWIKNCQNNPNPYLKGLFGLHSAFTLSDKTLSIVADKVRELNC
                     GIHIHTAEAESDEKYNLEHYGKRVVERLSDFNLLNNKSILAHCVHLNPKELILVAEKG
                     AAIVTNPQSNLNNAVGIADVCKMTELGVTVGIGTDAMTNNMLEEVRVGIWAQHLKQND
                     PSRGFVELAFALVFNNPLIAQKYWGAKFGTITAGSPADIIVVDYIPHTPFNKETWLGH
                     LIYGISQANVEATICQGKILMWNGELLLDIDEQEVKAKARELAEKLWDRF"
     gene            complement(15893..16417)
                     /locus_tag="PLW08_04290"
     CDS             complement(15893..16417)
                     /locus_tag="PLW08_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPL51854.1"
                     /translation="MKWWLLIILSLSLAFLGCEKTDQPTINEDGTKDNGKLESGGKNP
                     ESKTGVWLTDWNDAMKTADVLNRPVLVDFTGSDWCIWCKKLEEEVFGKGEFLEYAKKN
                     LVLLKVDFPRDINQSEELQKFNEQKMKEYGIQGFPTIVLVNSKGEEIARTGYRPGGAK
                     EYVKHLQELLQPKE"
     gene            complement(16414..16845)
                     /locus_tag="PLW08_04295"
     CDS             complement(16414..16845)
                     /locus_tag="PLW08_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPL51855.1"
                     /translation="MSTYVKTVWSGGMSFTAEVNGHKLIMDADPYFGGTDQGPRPKPL
                     LLAALSGCSGMDIVSILEKMGIKDYNLEMDARGESATEHPIIYHTITLTYNFQGNNLP
                     AEKIVKAVKLSLEKYCAVNAMLKKAAKIIPKIYINNLEVPL"
     gene            complement(16845..17300)
                     /locus_tag="PLW08_04300"
     CDS             complement(16845..17300)
                     /locus_tag="PLW08_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPL51856.1"
                     /translation="MADYAKKFHELITRLQMVLSEIDYAQKACLQAGRMECMLLNYLY
                     KVNGPANMNELAKELNVSHSRITRIMDNLVAKQLVIREPSEEDRRCWFARITEKGKKL
                     AANSLQTVLDQQAKILKKIPEKDIEAIYKALKLYVDKYEEVLKETIAGL"
     gene            complement(17293..17559)
                     /locus_tag="PLW08_04305"
     CDS             complement(17293..17559)
                     /locus_tag="PLW08_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPL51857.1"
                     /translation="MSAKVIVFSTPTCIWCKKAKEYLKSINQNFTDIDVSKDIAARND
                     MIRKSGQEAVPQIWINNIPVVGFDLEKINRLLNLNNTKKGESNG"
     gene            17761..19095
                     /gene="radA"
                     /locus_tag="PLW08_04310"
     CDS             17761..19095
                     /gene="radA"
                     /locus_tag="PLW08_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPL51858.1"
                     /translation="MATIFFCTDCGFETGKWSGKCPACGAWGTLKESTRVTGKNNKPV
                     ETLPLSKPEKIIDLEYDEFSRLLTGIKEFDLVLGGGIVKGMLILIGGEPGIGKSTLML
                     QLSEWMGKQNKKTLYCSGEESTEQIRLRSKRLKVTSENIYLLCTNNAEHIIEAIEENK
                     PDLVIVDSIQSVSIPSLDALPGTVTQLRETTSRLLRTVKNLGIPLFLVGHVTKEGLVA
                     GPKIIEHMVDTVLYFEGEQRSQYKILRAVKNRFGSTNEIGLFEMTHLGLIEVSNPNNI
                     FLSHEQNDIGTAISCIMEGSRSFIVEVQTLVNASNYGTPQRVAVGLDQKKLAMLLAIL
                     EKNLSLYLRNCDVFVNLTGGIRSTDPSLDLAILAALISSMKDQPLIEKAVFIGEVGLN
                     GEVRPVSQLDKHISQTLKLGYEKIFISSYAKVKANPKVVKVKDIKALYGALR"
     gene            complement(19289..21421)
                     /locus_tag="PLW08_04315"
     CDS             complement(19289..21421)
                     /locus_tag="PLW08_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPL51859.1"
                     /translation="MNRKLISEFKYDLAPVIRGYNKRSLYINLSTNEIKQKPVTELMI
                     DKFVGGKGFDLYLMWHSVQDDTKWDSPENEICISFGPLCGNTSYPGSGKSIVTTISPL
                     TGIPVDCNVGGHFGPYAKFSGWDAIELQGIANEEVIIYIDGDKGIVQILSAPEEEINS
                     HILAEELIKEFADGPDKYQFVSVVSSGKAAENVLITCLNFSFWDKRRKVARLKQAGRG
                     GTGSVFRHKKIKALVVKYSGLKVDSNNPEDAETLRMTGLKLHKEIEAGDATQNRMRQV
                     GTAHLMEIMNDYDLLPIRNFKYGQSPEAEGLHSREFKNLFTQGMPDGCWYGCSLSCCK
                     GVDNFEPRTGPYKGQKVCVDGPEYETAAGCGSNIGVFNARDVVEINFYCDTYGVDTIS
                     FGTSTAFAMECYENGILNKERTGGLDLTWGNADAALELLHQMAKGEGFGVIVGQGVRR
                     MKQIFAEKYGADPNFLQDIGMEAKGLEYSQYMSKESLAQQGGYTLALKGPQHDEAWLI
                     FMDMVNNQIPTFKDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPANNAETDEPAKVPE
                     HVQNYVDIYKAITGKPLDKVSLIEQSERVYNFQKVFCLRMGKGRRIDDVPPYRAVGPV
                     TEEEYLSRQERYDQQLKEKIGVDPEGKSTAEKMDILRKYREDQYQQLIDAVYERKGWT
                     KEGVPKIEHLQKIGMDLPEVIEVVKRFL"
     gene            complement(21432..21815)
                     /locus_tag="PLW08_04320"
     CDS             complement(21432..21815)
                     /locus_tag="PLW08_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL51860.1"
                     /translation="MKVLKTFPDKCITCHNCESACSKLYFKEDNAEKSCIVINEEVYP
                     PEMIVCNQCGNCIDICPTLALTINTQGVVMLNKSLCIGCLMCVAACPNNAMRFAKGVY
                     NPFKCIACGSCPKTCPAEAIELVTA"
     gene            complement(21876..23141)
                     /locus_tag="PLW08_04325"
     CDS             complement(21876..23141)
                     /locus_tag="PLW08_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51861.1"
                     /translation="MRNGIKIIIVCLFCTLTWSMQGETISPFSLKDKTFEARADFGIG
                     TLKNRNFSFWEGNSSFHYPQNIQAEMDYDSYSSFPEWSKNVTFHSAQEFLYLDVPKSG
                     GNIQEFNSFNTIGVKSRLAGVEVFYNLINNRNDTDEKATSYHFNKYGIGAGFPNSESL
                     RLFGWLMHTDLSDIYPEVDFSSYKAQMAKRYRSKSQIAELGMEYEYNEIPDKVSEEEL
                     LKVSEYFPDDYLDWTPKKRFSAYVYASSIRNFYLEPFFLNGHAPDCSPLLLADKSASS
                     VLCRFTTEDRKNDYVDNSQIVLDGILALNFGKYFGLDFRYDSEKKEDYKNDIKENTFN
                     ITGNMRLNFIGKESYRLIGILEYLYNKTKGENASENLNFGLYGILRLKNHLNLIAYLK
                     LDNEWQRKEDFLEFDHRDNKLGTILSVRL"
BASE COUNT         7035 a         4892 c         3934 g         7331 t
ORIGIN      
        1 aatctgcgta atctgcggga aatattatct gtgaaatctg tgagagaaaa cagctaagcg
       61 gttaaataaa attaattagc tggaaaacta tttcttctcc aattaccgac cagaaaaccg
      121 ctgctaaagc aataaaggtg ccaaaagcaa agggttggct tttatctcta ataaaaataa
      181 ggaaatagat tatccccagc aaggcagaaa gcaaaaagat gtaaggtatg cctgttaaac
      241 caaaataagt tgccagagct gtaaacagcc agatatctcc cccacccaat ccttcggttc
      301 cgcgtatttt ttgataagcc caagctaaaa atagaagcaa acagaaaacc actgctcctg
      361 ttactaaacc atttatgatg ccgacatcgt ttccaggtat aaaaacaaag atgatgccgg
      421 taatcaataa cggaatggac aacacattgg gtattatttg atgtaaggca tcaataaaaa
      481 agataggtat gagaaaagaa aaaagcagga tgtatttata gaaacggaaa tcatgcaaac
      541 cataacggaa aaacaacgct aaaaacaaaa gaggtgttat aatttcaact acaatatgat
      601 gccagtgaat tttagctcca catttggaac attttccctt tagtaaaata tagctgataa
      661 tgggaatatt ttgatagaaa ggaataggtc ttttacagtt ctcacaatga gaaccaggga
      721 aaattatgga ttgctttttg ggaatccggt aaatcaaaac attgaaaaaa ctgcctaaag
      781 cacaacccaa aacagcaata ataataatta caaaggttag cagataatgc tccttaccag
      841 ttttgcaggt agttgttctt atagggattc aaaatagaac cactaatcat ttgtaactga
      901 atttgaatgt tatcccgggg agtgtaacgc agggaaaatt ctggaataac acgactgtaa
      961 ttggcattat ccagttcaaa tttgctgctg gtatcaaaac cgccatagtt tacaaaattc
     1021 aagtccagat tcatttccag tttgggattg aatttatatt taagatgatt ggtataaaga
     1081 gaaagataat acccctcacc ggtggaactg gttcctgcct gaaaacccat agaatggctc
     1141 attgaaagtt tattcaaacc gagaaacgaa gaaccgttat tggtatatgg actgaaattt
     1201 ggatgcggaa tatcactttg agctattaac ggtaatagca ttactaacag catagtaatc
     1261 agcaagacct ttctcatttt tttctcctta gtgatgcaaa ggatttatat atattataag
     1321 caataattat accgatgtca aacataaatt gattttatca attcacccag gcgtttgatg
     1381 cccgtttcta tttgttccat agtagaatag gtataattaa gacgcataca gttaaatctt
     1441 tcctggcctt caggatagaa tttactaccc ggaataaagg ttaccaaatg gtccttggct
     1501 ttatagaaaa gggtatcggc attgatatct tcaggtagcc acagccagat aaaaattccg
     1561 cccttgggtt ttgtccattg cacataaaca ggcatctcgg cagaaaggca agccaacatc
     1621 ttgtccagat aaggtttata gaatttacaa agatttttaa tatgcggttc catataaccc
     1681 tcagccagaa atttggctac aactctttcg gttgtgcaat cgggactgat agtaactttc
     1741 tgttgccagg aaaccatttt attgatcaat tccggagctc caatagcgta ggcaacgcgc
     1801 attccgggac ccaatatttt ggaaaaagaa acgatttctg tcaccagata aggattattt
     1861 aattccgttt gagcgatgcg taataaagtg ggcagttctt cttcgctgaa atttaaacgg
     1921 gaataaggat tatcttccat aatgagaata tcttccgctt cacagtattt aagcaattca
     1981 tagcggcgtt tataactcat cgtaatacct gtgggatttt gaaaatcggg aatagtatag
     2041 agaaatttga tcttcttatc ggcatcgcgc aggcgtttaa ctttctctgc caaacgattt
     2101 aaatcaattc cttcattatc catagaaaca caatgaacct ctgctcctac agcttcaaaa
     2161 gcaacaatag aacccaaaaa actgggcgct tcgcaaagaa caacatctcc tacattaagt
     2221 aaagttctgc tcatataata aatggcattg gtggctccta cggtaataat cagattattc
     2281 cgattcaggt taggataatt ctcccacttt aaaatttcct ccttcagttg atcgtcacct
     2341 tcactgttac catattggag aacatctctg ccctgctgga aaaccacttc ctgaaataat
     2401 ttgctcaaaa tctcagtcgg aaaagttgcc ggagaaggaa aacctccagc aaaagagatt
     2461 aaatcaggaa catttttagt ggttgctacc agttctctga tgagcgatga tttcattgtt
     2521 ttcgtggttt ccgaaaagta attcgtaatc attcttctat ccttatattt ttatttttga
     2581 aactaatatt ctttcttgca ggaaaagacc tttgcccttc ctgcgtaagc agaaatcctg
     2641 ttgctcaggg ataaatccgg taaatcatac ggggaaaagg tatggtctct ctaatatgag
     2701 gagttccact caaccaagca accaatcgtt ccaatccaac tccaaaaccg ctatgcggaa
     2761 cagaaccgta ttttcgcaag tccagaaacc attgataatc atctataggc atattttccg
     2821 cttgcattct ggaaagtaaa agctcataat catcctcacg ctggctgcct ccaatcaatt
     2881 ctccaaagcc ctcaggagct attaaatcat ctcctaaaac caaattcgga tttaaaggat
     2941 cgcgtttcat ataaaatgct ttaatttctt tgggccagcg ttctacaaaa acaggaacct
     3001 ccgaatcctc cgttagcaag acctcatcct gcgctcccaa atctttatcg tgagtaatat
     3061 cactcccttt ttggcgtaac aattctactg cttcataatg agtcatccgc ttaaaaggag
     3121 catccgctgc ttttaatgcc tccgtatttc gttccaaaat ttgcagttcc agggcacatt
     3181 cggagagaac agttcggata acatagcgaa taaggtcttc ctgaatttgc atattctcat
     3241 cgtgttccac aaaagctgct tcggcatcca tcatccaaaa ctcggttaaa tgcttacggg
     3301 ttttggaacg ttctgctctg aataccggac caaaatcata aaccctgccg aaactcataa
     3361 ttcccgtttc cagatagagc tgccccgatt gcgaaaggta tgctttgccc tcgtcaaaat
     3421 aatccagttc aaaaagtgtg gtagtgcctt cacaagcatt aggagttaga ataggagaat
     3481 caaaacgaat aaaattatgc tcatttaagt aattgcaaat agcataataa acagtatgcc
     3541 gaatccgtaa aatagcccat tgcttttgag agcgtatcca aagatgcctg ttggaaagaa
     3601 gaaaatcggg tccgtgttct ttcttaccaa taggatattc atccgcaatt tgcaggggtt
     3661 gaacttgcgt aacttgaagt tcataaaccc cttcctgttt aggatggagc ttaggaatcc
     3721 cgcaaattat tacagatgat tcaagggtaa ggcgttttgc tgtttcaaat gcttcttctc
     3781 ccaaatcagg ttgaaaggca acggtttgaa tttctcctgt tccatctctt aaaattatga
     3841 acagcagttt tccgctatga cgaatattgc gaacccagcc cttaagccga acttcctgtc
     3901 ctaaatactt ttctatgtct tttacaaata catcttgcat tttcttttac ctgtgtaata
     3961 agtttattct ggtattttgg gtttgaattt gatttataat tttagtggct atttccaaag
     4021 ctctggttcc tgcttctcca tctacaaccg gttttttatt gtttttaaga caatcaataa
     4081 aagactccag ttccaaagtt aaagcatcct tgttaatatc gtcggcatca tagcttttaa
     4141 tatccaaaag ctgctccgga ttgatatttg gagctcccat cagaatttga ggtaataaac
     4201 tgtttatatc ggaacttttt ttgataacac gcacattttt tgccagaaaa tccatcctga
     4261 tataggcatc attctgaaaa aatcttaatt tgcgttcctg ttttgtggaa acgcgggaag
     4321 aagttacatt ggcaacagct ccattggcaa attcaatacg cgcattagca atatctatcg
     4381 tagaagtaaa gatacctact ccactggctt taatatcggt aaccgggcta tgcacaaagc
     4441 ttaaaatgag gtctatatcg tgaatcatta aatctaaaac aacaggaaca tccgttcctc
     4501 gcggctgaaa agtggaaatc ctctgcgatt caataaacag agggttattg atttccttct
     4561 ctattttcag gataacggga ttgaatcttt caatatgccc cgcctgaata atgagattcc
     4621 tttcggaagc aagcttaagc aagtcgttcg cttgagctaa ttcactcgta atcggctttt
     4681 ctacgaaaat atgctttccg gcaagtaatg cagttttggc aagttcataa tgagctgtgg
     4741 ttgtagcaga tatatctatt gcatcgcagg cagttaaaag ctcttcatag ctgttaaaag
     4801 acctggtatt tagcttttgg gctatttcct ttgcgcgttg ttcattgatg tcataaatgc
     4861 caactaattc cgcaccatca atgttttgaa acttttgggc atgatgttga cctaaatggc
     4921 caactcccac aactccgatt tttatcattt cttaagcttt tgcgaaagaa ccttcagcat
     4981 atctaaagtc attgcaggaa ggtcatattc cggtttccaa ttccattctt ccatagctgc
     5041 tgtctggtcc atagaattgg gccaggtttc tgctatggat tgtcttacag gatcaatttg
     5101 atagtcaatt gtgaattcgg gaatatgctt tctaatttcc ttagcaatca tttcgggttc
     5161 aatgctcatt gctgtaacat taaaacaatt acggtgcttt aactttttgg ggtctgcttc
     5221 cataagctct acacaggcac gcatagcatc cggcatatac atcatatcca gataagttcc
     5281 cggcttcaga taacaggtat aatttttatt tttaattgcc tcataataaa tctctaccgc
     5341 ataatcagtc gtccctcctc caggaagagt aacattggaa ataattcccg gataacgaac
     5401 tccccgcgta tccactccat attttaagaa ataatagtcg ctccaaagtt cggctgcaac
     5461 tttggaaatg ccataaatgg tagtaggacg cataattgta tcctgcgggg tattatccaa
     5521 gggcgtggaa ggaccaaatg cacctatgga agaaggtgta aaaacagcac ctttaacttc
     5581 acgcattatt tccagggaat tgatcaagct tcccatattt atgttccatg cttttaaagg
     5641 attagcttcc cctttggcgg aaagaacagc taccagattg attatagtat caatactgta
     5701 ttttttaact acttcaaata tcttatccgc ttccatagca tccaaatttt cacagggacc
     5761 gccttcccga atttccattg ttaaaggtgt gctacgatag gcagcaacaa ctgcatttga
     5821 accatagagc ttacgcaaat aaggaacaag ttccgaacca atttgtcctg ccgctccgag
     5881 aaccagaatg tttttcatct tctaactccg ttattttttt actttatgtt attaccttgt
     5941 tttttttctg ccctgccttg tcaattgttt ttcctgtaaa atgaactgac ccctcttttt
     6001 gaatgatatg cccataaaaa aatgatataa ccttatcctc tgtgtaaatc caatctcttt
     6061 ctctctttgt aaaacagcaa aacaatacat ttttttccaa ttccatctcg caaactggct
     6121 accctgccaa ctcacttttc tctttgttac acaaaacttc tcagctttgc tgctggctct
     6181 ttcactttgc tcataaaaat catcgcggca atcacaaatg gcttataacc cgcttcctga
     6241 taggtatgca aacggtattt ttcaaacacg ctttttgcca cttcaccatt ttcacaactt
     6301 acatcgctaa tatctgcagg aagacagtgt tcataaagcg catttccgtt ttttgtcaat
     6361 ttcatcatct cgtcatcgca ttcccagtca atatactttt cattttgagc caaacaatgc
     6421 agttccagtt ctttcaagcc ctgtttatca ccggctttaa gcaatttggt cctttgttgc
     6481 ataactgaca ttggtgccca acttttagga taaacgatat cggcattttg gaatgcttct
     6541 tccattaaag tggtttggct aaatgaacct ccgctctctt gagcaaatga aaaagccgac
     6601 tgtatggtct ccggaagcaa atcatagccc tcaggatgag ccaatacaac attcattccg
     6661 aaacgggtca tcagatttat tattccctgc ggaacagaaa gcggttttcc gtaagaagga
     6721 gaataagccc aactcatagc tattttcttg ccttttagat tttcccatcc gccaaaataa
     6781 tccttcagtt tcaaaagatc ggaaagaact tgcgtaggat ggtctaaatc gcattgtaaa
     6841 tttataacgg taggtctttg tgctaaaatt ccctgctgat aaccttcgct aatagcattg
     6901 gcaacttcca acatataagt atgcccctct ccgggataca tatcatcacg aataccgatt
     6961 acttcagtta gaaaggaaat catattagct gtttcccgaa ccgtttcacc atgcgcaatt
     7021 tgacttttca cttcatccaa ttccgctaaa gctaagccca aaccatttac tgccgaagca
     7081 aaactgaaac gcgttcgcgt gctgttatcc cgaaaaatag atattgctaa gccataatca
     7141 aaaattcgcc agggcttctt ttgccaatgc agacattgca ggatttcagc aaccagcatt
     7201 actgctttca agctgtccaa actgttatcc caggttaaaa ggaaatcctt accgaataaa
     7261 tccggtttta aggtttccag ttcattaatc agggtattaa tcttctccat attatacctc
     7321 tattttacat tttgtataaa aaatgaaagt gaannnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnntgcagcc aggaggatag catctataat ttgctggtat cctgtgcatc
     7501 tgcaaatatt tcctttaatt gcttctctgg cttcttcccg cgtgggctgt ttattttcca
     7561 gcaaaaaggc ataagcggtt aataccattc ccggagtgca aaatccgcat tgaattgctc
     7621 ctgcatcaat aaatgcctgc tgaacaggat gtaaagttcc atcggaagat gccagtccct
     7681 caatcgttaa gacctgttta tcggcaacat cttttaattt cagcaggcag gaacgcattg
     7741 gtttattatt taccagaatt gtgcaacttc cacaaacacc aatatcacag ccgattttgg
     7801 ttcccgttaa atccagattt tcccgcaaat aggcggaaag tttcagttcg gcaaggtttt
     7861 cggacacagt atattcaatg ttatttacag ttattttcat ttttgctttc ctttaacata
     7921 ttcccaaacc tgcggagcca taggtaggtc tgtaaatctt tttccggtag ctctgtaaag
     7981 ggcattggca attgctggag cggtaatttc caaagctggt tctccaattc cttttgcttt
     8041 ggaaggtgaa ttgggatcgg gattttccac aaaaatagct gtcatttccg gtaaatcggt
     8101 acttcgcatc actctatagc tgttaaaatt gcttgccttt ggttcgccat ttaccaaggg
     8161 gaaattttcg tatagagcat aacccgctcc cattgccatt ccaccataaa tctgaccagc
     8221 cagtaaagca ggattgatag ctctgccaat atcgtgagcg gcaactaaat tcagtaaagt
     8281 gattttccct gttttggaat tcacttttag ttccgctgcc tggcaactgt aaacccagga
     8341 aaaataagcg tttccatggc ctgtttcttc atcccagcta attggcggtg cctgaaaaac
     8401 tccgaaggca tagggataaa tttgatgatc ataacataaa cgaactgctt caaagtaatc
     8461 cgcaactttt tctccctgag cgaaatagac attaccatct ttataactgc aatcctttat
     8521 accaagagct ttttctactc cttcacagag acgctgttta attatttttg cagcattaac
     8581 aacagctcct ccccccataa tagttcctct ggaagcgact gtagaacccc catcgggaat
     8641 attggaagtg gaaggtaaac gataacgaat aagctcttta gggatgccaa gttcttctga
     8701 tgcaattaag atcattgcac tttgagctcc ctgaccgttt tcgtgaactc ccgtttccag
     8761 taaaacactg ccatcgggtt gaatataaat aatagcactg ttcatatcgg ttccttctgc
     8821 tcctaaacta actccccgat aagaaattgc ataaccgatg ccataccatt catctatctt
     8881 aggattgccg aaactgcatt tctttacttt attttcgtaa tcaatttctt ttaaaacgct
     8941 atccataacc tcttgcaaag aaacggtatg attatccagt ttctgatcgg taattgtctt
     9001 gctaccttga tgaaccatat ttatctttcg gaattctgtt tcatccatat tcaatttttg
     9061 agcagccatt tctattaact gttctatggc aaaatttact tgcggagaac cgaaacctct
     9121 gaaagctccg gaaaaaatgt tgttggtgta aacaccataa acatcactct gcacattggg
     9181 aacttcatag cagccacaac tttgaactgt ggaacgccaa gttacccaag gcgttacact
     9241 acaataggca ccaccgtctg caataatacg agctttgata aagacgattt tgccgttctt
     9301 tttcagtccc attctatatt gcagtttata aggatgccgt ttatagcttt cccgcatact
     9361 catttcccgg ctgtaagtta attttaccgg tctctttgtt agataggcac ataaagctgc
     9421 tctggcacaa accaaagccg ctgtatcatc ttttcctcca aaaccgcctc ccataggaat
     9481 atttttcact tccacttcgc ttagtttaca acccaaagtg gaagcaacaa atcttcttgt
     9541 agaaaaagga tgttgcatac tgccaagaac ttccataact ccatcagggc gtaaataaca
     9601 aagtgctgtt tccggttcca gataagtgtg ctctacgcgt gaagtattaa attcctgttc
     9661 cataataata tctgcttctt tttcaccctg ttcaatatca ccggttcgca ctttatgatg
     9721 gttacagata ttactaccaa aatcaggatt aaccaaaggg cttgtttctt gcattgcctc
     9781 ttctggatta taaacagcgg gaatttcttc cagttccact ttaattaaag gcaaagccga
     9841 aagagctatt tcccgagttt ctgccacaac taaggcaata acatctcccg cagaacgaat
     9901 ccttctatta attatagtgg gacaatcttt aataatctgg ccaaatttga tcttcccagg
     9961 gatatccttt gccgtataaa cagctaccac tcctttttgc ttaagcgctt cagagtaatc
    10021 aattgattta attatagcac ttgctacttg agaatgcaaa ggaacagcat aaagcatatc
    10081 aggaagttta taatcatcag tatattttgc tctgcctgtt actttggcag cagcgtcatt
    10141 acgaaatgca tcagtaaaca tcattatgat gcctcctcaa ttcataatag ctccaaaaat
    10201 caatccaggg attaggtgta tttgcccaac tgggaataac gggaattgga gctcccaagc
    10261 gttcataact aagcttatca ttttgaaccg aaaaacccat cagtttagcc ggattaaaac
    10321 ttataaaatt aaaaagttgc tccatactaa gcttgccttt ttctaccaaa tgctctgcca
    10381 gcaaagtaaa aagaactccg cttcctgcaa tgcccattgg ggttttggca ggatacaaaa
    10441 ttcctacatc tttggtttca gaactaaaag cacaatgatc actggcaata atatcaaaaa
    10501 caccatcctg taaaagttcc agtaaaagac ctctactgct ttcactgcgt aatggaggag
    10561 tacaaagcca acgatgacct tccggttcat ttaagacctc ttcattcaaa agcaaataat
    10621 gaggtgcagt ttcacaagta atataatccg cttctttttt tgcctgttga actaaaagcg
    10681 ctgactgagg agaagaaata tgcactatgt gaagaggata atgatgtttt atagaaaggt
    10741 caagcaactt ttccactgca gaaatttccg ccttttcagg acgccgcaaa cagtgatccg
    10801 aaggattatg aaaaggatag cgttcactat attcactaat aatttgatca tcttcacagt
    10861 gcaccagcaa tcttttttta ggggagggca tttcttccat caagtgttca atactttcat
    10921 agctttgata tagccctacc tcacgatagg tagtatagag cttcaaatcg gtatcttcct
    10981 taagaatgtt ttctaatctg cgaatatcca actgcctggg agttaaatgc cagaaaacct
    11041 ttcccaaaaa gctgcgggac acttttcgca ttcttcccag tttggaagaa agcggttcac
    11101 tctggctttc ggttacaaaa acaccaattc cttccaaacc gttatgttca taaagaagat
    11161 taaaactttt ccaactatct gctaaaaggt gtccaccaat ggtttcacca atatgcacat
    11221 ggaaatcgta aattccgctt gctttcatag ttttacggct ccagggtctt tcaaaccgtg
    11281 acctgttatc aataatacaa tatctttagc atcttttaac caactatgat ttactacctt
    11341 tttcagtcca gccaaagttg ctgaagaaga gggttcacca aaaacacctg tttcttttgc
    11401 cagttccagc tgagcttcct gaatttcttt atcgcttacc ctgatgcatt ttccgtctgt
    11461 ttcattaagt gctttaacag accaatgcgc taaagcaggt gtcttcactt taatagaatc
    11521 ggctatagta atagggtttt cagcatcgtg ataaattcct gtttcccaat aggaagtaat
    11581 ggcatcactg ctttctgcct gaacacataa gagatgaggc attttttctg ttattccggc
    11641 tttttttaag tccaaaaatg ctttgtagat acctgttaaa ataactccat cgccaaccgg
    11701 aatcacaatc caatccggaa ctttatatcc attttggaca taaatttcaa ttccggcact
    11761 ttttttacca tctatggtta aaggatgata gcccgtattg cgacacatac aattatgcgt
    11821 ttttgtatat tccaacgcgg ctttgaaagc atcatcataa ctgccatcaa tcttatgaag
    11881 ttccgcatta tgcacctgaa tttgaattaa ttttgcagga ggagcatttt gaggagcaaa
    11941 aataacaact ttcaaaccgg ctgaagcacc tacacaagca agtgaacaag cagcatttcc
    12001 tgtgctggca gttacaattt ctttaatccc tttttgtaaa gcatcagcta caacaagttg
    12061 tgaagcacga tctttataac taccgctagg atttaaaccg tcaaatttta tccacaattt
    12121 tgtgtagtcg gaggacgccg tttctccgct tttcaaacca ggaacattaa ccctaaaaaa
    12181 aggtgtgttc cctacaggta aaggaggata aaatttagga tttacagcac tgaaaagaag
    12241 agggtcagga ttttgtttcc attttctgcc aatttcttca tagttaaaaa ccgcttccaa
    12301 aactcctttt aatggcattc caggtagata atcccggctg cattcatcac aaagataatg
    12361 cagttcatta gggtcaaatt ctttcccgca ttcaatacag cggtatttag ttaaataact
    12421 cttcatctat ttctcgcaaa gatttcgcta atttcgcaga taaaagaata gcaccaggat
    12481 ttattccgat taacacagat tcttctttat aattttcaat tgtcaattct tctctttctc
    12541 tctttttctc tttgtgaaaa aatccatctc acttttcctg tgaaaatcta taaaccagag
    12601 cagcataaaa agcagcagct ttggttaaat gttcaatcgg gcatgcttca ttaggtgaat
    12661 gagcataaat ttcattcccg ggacctaatc caatacaggg aattcctttc attcctgcaa
    12721 tagaaacacc attagtggaa aaagtccatt tatcaataag tggctttgtt cccagggttt
    12781 ttgtgtaagc atcagcggca ttttgaacat aaaatgaatc caaaggtgta acccaagtag
    12841 gataatattt ttctgtagga taaaccaatc ctgtatatgc tgcctcctga taagttaaaa
    12901 tttctattgt agcatcagga taaccggctt ttttacaggc atcctgaact tcggcaacag
    12961 cgctttcttt attttctcca aacgttaaac gtctgtctaa atgaatgcgt gcactatcgg
    13021 gaacagcaca ttggcttggt cctgtaaaaa agacctgagt tacgcaaata cttccttttc
    13081 ccaaaaaatc atcataacgc agattttgat gcaatttctc tatttctaaa gcgatacggg
    13141 aaattttata aatggcatta tctccgcgtt caggagcaga accatgacag gaaagtcctt
    13201 taatgctaac ctccatttcc attctacctc tatgaccccg ataaatattc aggtttgtag
    13261 gttctgtaat tacaacaagt tcgggtttaa tttctccttc ttgaagaata tactgccagc
    13321 ataagccatc gcagtcctct tccataactg ttcctgtaaa ataaatgcta aattcctttc
    13381 ccaaatccaa atctttcatt atgcgggcag aagtaagcat acttgccatt ccgcttttct
    13441 gatcaacagt tcctctaccc caaatttttc cgtctttgat gtgagcagca aaaggatcaa
    13501 aatcccaaag agaaaggtct cccggataaa cagtatcaat atgcgcgtca aaagcaatca
    13561 cttttttacc aaaacccaaa cgaccaataa cattccccaa agggtctata taggcatcgt
    13621 ccattcctat ttcctgcatt tctttcagga tgcattctgc aacttctttt tctttagtag
    13681 agaaagaagg tattttcacc atatccatta aacaacgggt gctggtatct tcataatgtt
    13741 ttgcccttgc aaaaatctct ctatacatta tcaatctcct tatgttttta cggtaaaaaa
    13801 tttaacgccg attctgcaaa tttatttttt atcccgaggg ttatttccaa ttccaacagc
    13861 gttattttcc ctttttggac attcttcatt ctaatttctt ctcttttgct cattgtaaaa
    13921 aaatttattt cgcttttcaa aatctgtccc acaatttttc cgctaattct cttgctttgg
    13981 ctttaacttc ctgttcatca atatccagca gcaattctcc gttccacatc aggattttgc
    14041 cctgacaaat agttgcttcc acatttgcct gggaaattcc gtaaatcaaa tgtcccagcc
    14101 atgtttcttt attaaaagga gtatgcggaa tgtaatcaac cacaataata tctgcaggac
    14161 tgcctgcagt tattgttcca aattttgccc cccaatattt ttgcgcaatt agcggattat
    14221 taaaaaccaa agcaaaggca agttcaacaa agccacgcga gggatcattc tgtttaaggt
    14281 gttgagccca aataccaact ctgacttctt ccagcatatt attggtcatt gcatctgtgc
    14341 caataccgac agttacgccc aattccgtca ttttacaaac atcggcaatg cccacagcat
    14401 tattcaaatt agattgcgga ttagtaacaa ttgcagcgcc tttttccgcc accaggatca
    14461 attccttggg gtttaaatgg acacaatgag ccaggatgct tttattattc aacaaattaa
    14521 aatcgctcaa gcgttccaca actcttttac cataatgctc cagattgtat ttttcatccg
    14581 attcggcttc cgcagtatga atatgaatac cgcagttaag ttcccgaacc ttatcagcta
    14641 caatagatag agttttgtcc gaaagcgtaa aagcggaatg taacccaaaa agtcctttta
    14701 aataaggatt aggattgttt tgacaatttt ttatccagtt atagttttcc tcaattcctt
    14761 cttgggcaat tttatctccg tctctatctg aaacctcata gcacaaagat gcttttattc
    14821 ccgtttcttg aaccgctttg gcaatttgag aaagagaacc tttcacggca aaaggggaag
    14881 catgatgatc aataatagtg gttgttcctt tccgaatgga agtaaggata gaaattaagg
    14941 cgctataata gttatcttca gaaagcagtt ttttatccaa acgccaccac aaattttcca
    15001 gcacagcatt aaaatttttg gcaggagcag cttttcccaa gccggttaca aaagtagaat
    15061 agaaatgatg atgagcattt ataagcccgg gcataatgat tttgccttct acatctatgc
    15121 gttcacaaac ctcatcggaa aagctttttt ctgagccgat ttgcaggata taaccatcat
    15181 caattaaaat ttctgcttgc tctaaaaccg gatggctact atcaaatgtt agaattgtgc
    15241 cacctttcag tatgtatcta ttcatcttta ttcctctctt taaactttat ctaagcagaa
    15301 aaaggaggat ggctatattt gtcaagctgg aatgtaggaa aatagtaaga gcgtaatctg
    15361 tgagatttgc acaaggagat tagcttcaaa aatataaggt tcatacttag ctgactgtat
    15421 attactgaag taacaaccaa ctaacaactc cgtaacgaaa ctactacagt gttacggaag
    15481 tgttacagag tcgttagata atttactatg ttacaagcaa ttactcacat tttattgtag
    15541 gagacaaaag aaagggataa tttatagata tgtaaaaagt taagctattt tggaggaatt
    15601 tttagtgcag gggtgattac aaatatttaa tttgcgttta tctgttatat ccgttaaatc
    15661 cgtgttgcta attttaaagt tgtcacaagt tattgtatag ccgtagctcg ttaaaactcc
    15721 gcattttttt gttgaggaac atcattctcc tgctacacat taaaaattcc caataaaaaa
    15781 aagacgggga catcatatta tcctgtgctt actgtttgga gtatcttatc accagagtat
    15841 ttttgtctcc gtccaatatt gaaagagatt ttgttattaa gcaggtaatt atttattctt
    15901 ttggctgaag taattcctgt aagtgtttaa catattcttt tgctcccccg ggtctatacc
    15961 ccgtgcgagc aatttcttca cctttagaat ttacgagaac aatggtggga aaaccttgaa
    16021 tcccgtattc tttcattttt tgttcattaa atttttgcaa ttcttcactt tgattaatat
    16081 cacggggaaa atcaactttc agcaaaacga gatttttttt ggcatactct aaaaattcac
    16141 ctttaccgaa gacctcttcc tccaattttt tacaccagat acaccagtca gaacctgtaa
    16201 aatctactaa aacgggacga ttcagcacat cagctgtttt cattgcatca ttccaatcag
    16261 taagccacac accggtttta gattcaggat tttttcctcc ggactctaat tttccgttat
    16321 cttttgttcc atcttcattt attgtaggtt gatcggtttt ttcacaacct aaaaatgcta
    16381 aagacaaaga cagtataatg agaagccacc atttcataat ggaacctcca aattgttaat
    16441 atagattttg ggaattattt tcgcagcttt tttcaacata gcattcacag cgcagtattt
    16501 ttccagggaa agttttactg cttttactat tttttcagcg ggaaggttat tgccttgaaa
    16561 gttgtaagtt aaagttatcg tatgataaat aataggatgc tcagtagcag attcaccccg
    16621 agcatccatt tccaaattat aatcctttat tcccattttt tccagaatgg aaacaatatc
    16681 cattccgcta caaccactta aggcagcaag caataaaggt ttaggcctcg gaccttgatc
    16741 agtaccacca aaatagggat cagcatccat aatcagctta tgtccgttga cttcagctgt
    16801 aaagctcatc ccaccagacc aaacggtttt tacataagtt gacattataa tcctgctatt
    16861 gtttctttca atacctcttc atatttatcc acatagagtt taagtgcctt atagattgct
    16921 tcaatatcct tttcaggtat tttctttagt atttttgcct gttgatctaa aactgtctgt
    16981 aagctgttag ctgccagttt tttacctttt tcagtaatcc gggcaaacca gcaacgacgg
    17041 tcctcttccg aaggttcacg aataacaagt tgtttggcaa caaggttatc cataattctg
    17101 gtaattcggc tatgagaaac attcagttcc ttagccagtt cgttcatatt ggcaggaccg
    17161 tttactttat aaaggtagtt taacagcata cattccattc ttcctgcctg taggcatgct
    17221 ttttgagcat agtcaatttc actcaaaacc atttgcaggc gagtgatgag ctcgtggaat
    17281 ttttttgcat aatcagccat ttgattctcc ttttttagta ttatttaagt ttaataaacg
    17341 attgatcttt tccagatcaa aaccgactac tggaatatta tttatccata tttgcggaac
    17401 agcttcctgt ccgcttttgc ggatcatatc attccgagca gcaatatcct tggaaacatc
    17461 tatgtctgta aaattctggt ttatggattt tagatactcc ttcgctttct tgcaccagat
    17521 acaagtagga gtggaaaata caattacttt tgcagacatt ttaatctccc tatttcacat
    17581 tataacgcaa aatttcccca aagcaaaaaa tatttttgcc gattaaaaat tacgcttgaa
    17641 caacggtaaa aaaacttaac ccttaaaatg ctattataga taactatcca gcaggatgct
    17701 taattttctt gccataaaat aacatcagat tattatggca aaaaatttga ggttgatttt
    17761 atggcaacta ttttcttctg cactgattgc ggttttgaaa ccggtaaatg gagtggaaaa
    17821 tgccctgcct gcggagcttg gggcacttta aaagaatcta ctcgtgtaac gggaaaaaat
    17881 aataaacccg tagaaacatt acctctaagt aaaccagaaa aaataattga tctggaatat
    17941 gatgaatttt ctcgcctact gaccggaata aaagaatttg acctggttct gggtggagga
    18001 attgtaaaag gtatgctgat tttgatcggc ggtgaacccg gaattggtaa atctactctt
    18061 atgctgcaac tttctgaatg gatgggtaaa cagaataaaa aaacactcta ttgttcgggt
    18121 gaagaaagta ccgaacaaat tcgcttacgc agtaaacgtt tgaaagttac aagtgaaaat
    18181 atttatctgc tatgtactaa caatgcggaa cacattattg aagcaattga agaaaataaa
    18241 cctgatctgg taattgtgga ttctattcaa tcggtaagca ttccttcact tgacgccctt
    18301 cctggaactg tcactcaatt aagagaaact accagtcgcc tattacgaac ggttaaaaac
    18361 ttaggtatac ctctattttt agtaggtcat gtaaccaaag aaggattagt tgcgggaccc
    18421 aaaattattg aacatatggt agataccgtt ctctattttg aaggagaaca acgcagtcaa
    18481 tacaaaattc tgcgagcagt aaaaaatcgt tttggttcta ccaatgaaat aggtcttttt
    18541 gaaatgaccc atctcggttt gatagaggtc tccaatccca ataatatctt tctcagccac
    18601 gaacaaaatg acatcggaac tgctattagt tgtattatgg aaggcagccg cagtttcatt
    18661 gtggaagtgc aaaccttagt aaatgcttcc aattacggaa ctccgcaaag agtagcggta
    18721 ggactggacc aaaaaaaatt ggctatgctt ttggctatct tggaaaaaaa cctctcactt
    18781 tatcttcgca attgtgatgt ctttgtaaat ttgacaggtg gaatacgcag taccgatcct
    18841 tccttggatt tggctatttt agctgctcta atttccagta tgaaagatca accactaatt
    18901 gaaaaagccg tatttatcgg ggaagttggc ttaaatggag aagttcgtcc tgtttcacaa
    18961 ttggataaac atatcagcca aactctgaaa ttgggttatg agaaaatctt catttccagt
    19021 tacgctaaag taaaagccaa tccaaaagtt gttaaagtaa aagatatcaa agctctatat
    19081 ggtgcgttaa gataactttc gcataaaagg aataaaggat tgtttcacag agagataaag
    19141 agaattaatt gacatttgag aatgtaatac aggagtaatt tgcgccttaa taaatttttt
    19201 gcatagagat aactttaacc aagcttatga cttatgacga caaatttcca aatatctgtg
    19261 caatccgtat tatctgtttg agcatatatt acagaaatct tttcactact tcaataacct
    19321 caggtaggtc cattcctatt ttctgcaaat gttcaatttt aggaactcct tcttttgtcc
    19381 aaccctttct ttcgtaaaca gcatcaatca attgctgata ttggtcttct ctatattttc
    19441 ttaaaatatc catcttttca gcagtgcttt tcccttcggg gtcaacacct attttttctt
    19501 tcaactgctg atcatagcgt tcctgacggg aaagatattc ttcttcagtt acaggaccca
    19561 cagctctata gggtggaaca tcatcaattc ttcttccttt tcccatccgt aaacaaaaga
    19621 ccttctggaa attatagacc ctttctgact gttcaatcag agaaacttta tccagtggtt
    19681 taccagttat agctttatag atatcaacat agttttgaac atgttccgga acttttgctg
    19741 gttcatcggt ttcagcatta tttgccggtt caatatcatt ccagggaagt ttgcataatc
    19801 cctgtaaacc aaaccaggtt ctaaacatcg gaaaataatg taatgcctca gctttatcct
    19861 taaaggtagg aatctggtta ttcaccatat ccataaaaat cagccatgcc tcgtcatgtt
    19921 gaggtccttt taaggcaaga gtataaccac cttgttgagc caaggactct ttactcatat
    19981 attgtgaata ttccaaaccc tttgcttcca taccaatgtc ttgcaggaaa ttgggatcgg
    20041 caccatattt ttctgcaaaa atctgcttca ttctgcgcac accttgtcct actataactc
    20101 caaaaccttc tcccttagcc atttgatgta aaagttccaa ggcagcatct gcatttcccc
    20161 aagtaagatc aagtccgcca gtgcgttctt tattcaaaat accgttttca taacattcca
    20221 tagcaaaagc agtgctggtt ccaaaagaaa ttgtatccac tccataagta tcacagtaaa
    20281 aattaatttc tactacatcc cgggcattga aaacaccgat attacttccg caacccgcag
    20341 ctgtttcata ttcaggaccg tccacacaga ccttttgacc tttgtagggt ccggttcttg
    20401 gttcaaaatt atccacacct ttacaacagg aaagagaaca accataccaa caaccatcag
    20461 gcataccttg agtaaaaaga ttcttaaatt ctcgggaatg taaaccttcc gcttccgggc
    20521 tttgaccata tttaaaattg cggataggca gtaaatcgta atcattcata atttccatca
    20581 aatgtgcagt tcccacttgg cgcatccgat tctgagtggc atctccagct tcaatttctt
    20641 tatgcagttt caagccagtc attctaaggg tttcagcatc ctcgggatta ttagaatcca
    20701 ctttcagacc ggaatattta accaccaatg cctttatttt tttgtgtctg aatacactac
    20761 ctgttccacc tctacctgcc tgtttcaaac gagccacttt tcttctttta tcccaaaagg
    20821 aaaaattcag acaagtgatt aaaacatttt ctgccgcttt gccgctggaa accacagaaa
    20881 caaattgata tttatctggt ccgtcggcaa attctttaat caattcttca gcaagaatat
    20941 gactattaat ctcttcctcg ggagcggaaa gaatttgcac aatgccttta tcaccatcta
    21001 tataaattat cacttcctca ttagcaattc cttgaagttc aatagcatcc cagccagaaa
    21061 atttggcata aggaccaaaa tgaccaccta cattacagtc cacaggaatt cccgttaaag
    21121 gcgaaatagt tgtaacaatg gattttccgc tacctggata actagtattt ccacaaagcg
    21181 gaccgaaaga aatgcagatt tcattttcgg gactatccca tttggtatca tcttgaacac
    21241 tatgccacat cagataaagg tcaaaacctt ttccgccaac aaatttatca atcattagct
    21301 cggtaactgg cttttgtttt atttcattag tgcttaaatt tatatacaat gagcgtttat
    21361 tatagccacg aataacaggt gccaggtcat atttgaattc ggaaattaat ttacggttca
    21421 tttttctctc cttaagcggt tactaattct attgcttcag cgggacaggt tttggggcaa
    21481 cttccgcaag caatgcattt aaaaggatta taaacacctt tagcaaaacg cattgcatta
    21541 ttcggacagg cagctacgca catcaaacag ccaatacaaa gggatttatt caacataact
    21601 accccttgag tattaatagt taatgctaaa gtagggcata tatcaataca attcccgcat
    21661 tgattacaga caatcatttc cggaggataa acctcttcgt taattactat gcacgatttt
    21721 tctgcattat cctctttaaa atagagtttg gagcaggcgc tttcgcaatt atggcaggtt
    21781 atgcatttat caggaaaggt ctttagcact ttcattataa aaattccccc ttttctttaa
    21841 tgattccata tcaaaatctg gaaatattat gtcaactata atctcacgga aaggatagtt
    21901 ccgagcttat tatctctgtg gtcaaactct aaaaaatctt cctttctttg ccattcatta
    21961 tccagtttca ggtaggcgat taaattcaaa tgatttttca agcgtaaaat tccatataat
    22021 ccgaaattaa gattttcaga ggcattttca cctttggttt tattatataa atactccaaa
    22081 ataccgatta gccgatacga ttcctttccg ataaaattta accgcatatt tccagtaata
    22141 ttgaaagtat tttcctttat atcgtttttg taatcctctt tcttttcgga atcatagcga
    22201 aaatcaagcc cgaaatattt cccgaaatta agggcaagaa tgccgtctaa aacaatctgt
    22261 gaattatcca cataatcgtt ttttctgtct tctgtggtaa atctgcataa cacactactg
    22321 gcagatttat cggctaataa taagggactg cagtcaggag catgcccatt taggaaaaac
    22381 ggttccagat aaaaattccg tatactggaa gcgtaaacat aagcgctaaa ccgtttttta
    22441 ggagtccaat ccagatagtc atctggaaaa tattcagata ccttcaatag ctcttcctct
    22501 gatactttat ccggtatctc attatattca tattccattc ccagttctgc tatttgactt
    22561 ttacttctat agcgtttagc catttgagct ttataggacg aaaaatcaac ttcgggataa
    22621 atatcgctta aatcggtatg cattaaccag ccaaaaagtc gtaatgattc cgaattagga
    22681 aaaccggcac ctattccata cttattaaaa tgataacttg ttgccttttc atctgtatca
    22741 ttacggttat ttatcaagtt ataaaagacc tctactcctg ctaatctgga ttttacacca
    22801 atagtattaa atgaattaaa ctcttgaata tttcccccac tctttggcac atcaagatac
    22861 aagaattcct gagcggaatg aaaagtgaca tttttactcc attcgggaaa actggaataa
    22921 ctatcataat ccatttccgc ttgaatattt tggggataat gaaaggaaga attgccttcc
    22981 caaaaagaaa aattcctgtt cttcaaggtt ccaattccaa aatcagctct tgcttcaaat
    23041 gtcttatcct ttaaggaaaa aggagaaatt gtttccccct gcattgacca agttaaagtg
    23101 caaaacaaac acactattat aatttttatt ccgtttcgca tttattactc ctctttaaga
    23161 taatgcttat ttttagggct ggctgttgct gtcaatggtt ttttgaggaa aagatagaga
    23221 aatggtgatt actttaatga aaaattgaga atggaaaatt ataaatcctt tctaatatat
    23281 atctgtgtta tc
//
LOCUS       DAOOZV010000029        22488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2443_length_22488_cov_54.695761, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000029 DAOOZV010000000
VERSION     DAOOZV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 22488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22488
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2443_length_22488_cov_54.695761"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLW08_04330"
     CDS             complement(<1..421)
                     /locus_tag="PLW08_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51862.1"
                     /translation="MKRYLYLIFGILLMLALGSCESNDSDTIAQREIRDILYDISMEF
                     NLKNISGIMEHLHIKYLHNGMTSYNFNDLWLDRMAQYSLLEIEVLYIDLDDDYAVVHS
                     KNKFTSHGETVILNEPEDSGDISYFKRENGVWYIYGNQ"
     gene            complement(445..1815)
                     /gene="rlmD"
                     /locus_tag="PLW08_04335"
     CDS             complement(445..1815)
                     /gene="rlmD"
                     /locus_tag="PLW08_04335"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPL51863.1"
                     /translation="MKHILNLEIEKMAMGGFGIAYAEGKAIFVPYTAVGDVVDIDITI
                     EKKDYAYGKVTKYISYSDSRVEVQCSSFAAEIPCGGCDWLMLKYSEQLKQKNQLLLEL
                     FSPFIDKDVIYFPAPSTQIKNYRNKVFMPVGKNYQTGKITYGMFARFSHQIVEHDTCE
                     NHPPIFDQLANSLMEFCLKAKVEPFNEIEHKGQLRHIGFRCNSDSRQILVILVTLSGK
                     LPFTNLLVKKLTTEFPQIKGIIQNINRQKGNVILGKETKLLYGSENLYDNLCGLKFRI
                     NYRSFWQINTGTMEMIISFLQDFVNSNDVILDAYCGIGAIGLSLADKVKRVILLEEFP
                     QAIEDAKENAVLNGLNNVSFVVGKVEDNLSAVLIKEKPDTIIMDPPRSGVQQNALEAI
                     IQAEVKQIIYLSCAPMTLARDLNTLIGGEKYKVVFLQPFDMFPNTWHIECLAVLKYND
                     YNINMR"
     gene            complement(1812..2681)
                     /gene="htpX"
                     /locus_tag="PLW08_04340"
     CDS             complement(1812..2681)
                     /gene="htpX"
                     /locus_tag="PLW08_04340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPL51864.1"
                     /translation="MNFLKRMGIFLLTNILVMLVLGIVVGLLGLKMEDLWGLLIICAI
                     FGMGGSLISLFLSKPMAKASYKIQIVKPGIAHPKIAYLLSAIESMAEERNIKMPEVGI
                     YNSRDANAFATGASQNNALIAFSSALIDRLSEEELAAVAGHEMTHITNGDMVTMTLLM
                     GVVNTFVMFFARILTVVIDNALRDDRGGGLGYFGYIMVIMVLQNILMLLANIPIAAYS
                     RWREFKADAGSANLVGAGPMIEALKKIANAYVPEKRSDSYALAKINNQKRISLFATHP
                     PIEVRIQHLQEML"
     gene            3046..4389
                     /locus_tag="PLW08_04345"
     CDS             3046..4389
                     /locus_tag="PLW08_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPL51865.1"
                     /translation="MKHSKIEILEKTPVAYSILYLALPSMLSMLVNILYNLTDTFFIG
                     KLNDPFKVAGVSIALPYFNMLMAIAGIFANGGASYLSRLLGKKDLKTARETTTTAIFT
                     VAIVSIFAATLGVLFIPTYLNLSGASALTALSARKYLTAIFIGSPIIMIKFTLIQLLR
                     AEGSAKEAMLGLFIGTGANIILDPLFIFTFKMDVTGAAIATVIGQGLAMLYYISYYLK
                     KKSLASPGRKYLHPRWSCYKEIFAIGIPSSLSQIMMSIGNAISFKLASAYTDHTIAAL
                     GVVSRVFSIVIFAFIGLSIGIQPLIGFNYGAQNYTRMKKVIKMSSIICLGMSAVFTLF
                     FALFPGELIAVFIKDKAIMKIGTQILNAYVFAIPFAGLGMILMATLQAMGKAIPAFIV
                     SLSRQGIVYIPMLYLLNYLFQFSGLIYAMPIADVITTILSFTFVYHITKRLKSSP"
     gene            4454..5788
                     /gene="rocD"
                     /locus_tag="PLW08_04350"
     CDS             4454..5788
                     /gene="rocD"
                     /locus_tag="PLW08_04350"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPL51866.1"
                     /translation="MSTNLKCDCSTDNEIVEVEKNYGGKMSQNLKYGNISSSEAMELE
                     EKYGAHNYHPLPVVLAKGEGVYVWDPEGNRYYDFLSAYSAVNQGHCHPKIIQALIDQA
                     KELTLTSRAFYNNKLGEYEKYITEYFGYDMVLPMNSGAEAVETAMKLARKWAYNVKGV
                     ASDSAILIFADNNFHGRTISIISASSDPDCFGGFGPFTPGIVRIPYDDANALKEYLEK
                     NGKNVAAFIVEPIQGEAGVVVPKDGYLKDCYDLCHQYNVLFIADEIQSGLGRTGKLLA
                     CDYENVRPDVLILGKALSGGVLPVSAVLADKDIMLQIKPGEHGSTYGGFPLACVVAIA
                     ALEVIKKEKLTERSFELGEYFRNELRKIEHPMLKLVRGKGLLNAIVIEPMNGFEAWDV
                     CLKLKDKGLLCKPTHRHIIRLAPPLIITKEQLDECIQIIKDVFNDLSANLGQ"
     gene            5834..6718
                     /gene="era"
                     /locus_tag="PLW08_04355"
     CDS             5834..6718
                     /gene="era"
                     /locus_tag="PLW08_04355"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPL51867.1"
                     /translation="MNNSANFKSGFVTIIGKPNTGKSTLMNLILGEKISITSPKPQTT
                     RYAIKGIWNTSEHQIIFVDTPGYLKPRYELQEKMLKIWHNALKDVDLIIFLTQIDGFP
                     TEYDKEVLNQLKTLKNPQLAVFNKLDLNPEVDRNELVKYLPESINEVFFVSAKTGENI
                     PELMEAIKYYIPFHEPYYDNDMLSDLPMRFFAQEIIREAIFHFFEEEIPYSSAVLIER
                     FKEFPDKVIIDAVIWLERESQKPILIGKNGSSIKKVREYAERELTRFMQMPVQVHLFV
                     KIKPKWRKKQSALKELGF"
     gene            complement(6746..13138)
                     /locus_tag="PLW08_04360"
     CDS             complement(6746..13138)
                     /locus_tag="PLW08_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51868.1"
                     /translation="MTKKLFACLILLALTILWATETVPDGSNPPEDDQTEQKGIFPAR
                     YFRPKKEIKHELDPLTEKALYPRLYLTEYNPIINNRIEVYKINKGMNYYKTKPYEIPA
                     FAPEYKEEIDFANGKVILSVQVGNYKIAPDVPISFDRYFSNMQTKVFHKSLIANFKTQ
                     EQVVQTVTSGLFKDLTLLPEIAMPKAMQKVLGSSAGRLNLDGTQKLTMQVSNTHRKQV
                     PIYDQSGKNVFDLKMEMETNLRLSGTIGDKIAVNFKYNSKQDEELFDMNNVNVKYTGY
                     DDEFIQSIEGGNIALSLSGSRYVSYSASSQGLFGITSKFKYGNLDLSVIASKEESQKN
                     TQTYIGKSQADSSTVSSWKYARRTMYYLHDPYQLYQLKTGTNIPPGWINNAIETAPDG
                     SWMVNVNFLPANGTVRLFYDDANYTNNTASAVGDTIYYSNNMRPPYEPWYDELIEGTD
                     FVTDYDSGIITILKTIDRTATLGVQYTRRDGVQVPPPNSDTSRLHVKVLRKKYQEYIP
                     YNPNDELNTWHLQMRNIYDMGRTNIKNDGFELQVYTVNVDLTRNYNLPDNLVFGDIST
                     YNDYLRLNANGSPDGLINGDDATVNLTNGWIIMPFIEPMRGLGDGIIYRKENEDEYSY
                     QDSTSIFINIKGKIGREAIELSSMGVLKGSVKVKVNGNEQRENIDYIVDYDFGRITFL
                     TAAGKDPDAKIEIEFESRTLFSVAQKNLAGIRANWKLSDYAKLGGTLIYRSEDVSDRR
                     PRIGNENIEMLMGDVDGELTFKPAFITKWLDALPLINTTAPSQLSLSGEVAFTMPNIY
                     GDPKSKKKEAYLDDMESIMDSYPLGVTYNTWMLGSKPFSTSYAKGRINWFNPKDVKRK
                     DIEAEATLTEQEKDEYVTVLTMIVKPNPVQIPGSTTHSWAGIMKYLGNQLDFSQKKYL
                     EVYAKLEPLYPNTNIYPNVTMHIDLGDINEDFYTEYGGYGVLNSEDVNRDGVLTISED
                     IGLDGIPKNQPGHDPNDIAYPASGNDYSGVNGTEENRILDTEDLDGNGVLNTLDRYLS
                     YSFSLTSPDSSLVVDSYNQWRMFRIPLNDEQYYQMVNSSGTNIQPSLQKISYARIWLD
                     TDAQMDVKVRIADISVVGNKWQDNFIRQFKYGNAPPPNSYVQYYNPIIEPSVLIAKNT
                     SYISGIVTNQKNFTHYTSPAGTWYTEEKKESAESALTLEVNHLQRDQMCLLRQRMLDT
                     QNLLSYDKIRFWVYPEAAQGSYSNLDSLYIIFRIGADSLNYYQVRQRVPVRPYNAKMN
                     ANDWIQLEYNLQDLISLKEINPNAEADSLVNIIPGDEGQLGKYYRGKPSLINIRDIYL
                     GVYVPGVHFYANQMDIKEFSGIIYFNDIRVADPYEDLGIAKRLSVSGTFADVATINVD
                     FEDKSENFNTTIQRGRSNIFTSTQSLNITNKIFLNKLFPNSWNLDMPLSLTRNYSLGT
                     PRFRANSDLLRANIADPELKEIEKTERLAYSADFGFSQKQAPKNKILQYTVYRTSLSG
                     RIESSYNNTSTAVDTILAYRGTLNYNLNVPAEKTSFKLFKNYRLSYFPNTFSNSVTFS
                     SNEPKSWDRLTTVDSLVWNKRSQTTDTRTITTDNNLSWSLLSDLTATARVNTKRDLLQ
                     KDYLYDINIGKQTEYVQDLGLNYSPNYLPQVFNFTSSVSARYTDTQRKYTQYVESQAV
                     DTYQRDGNTNRSIRMNLTLMNSSLLSNWAMKMKSKLPPESVSPKGKEDEGSAKTEMTE
                     EDKKKQEEQKKQEEKKKEGEQKKQEEMKKEEEQKLSEEEIQKRKDELARLEAEGKLAD
                     LSEEEVNKLMEDIFGKGEKEEKTEEEEKETETTKPSETKEPGFNPVITLVNALAMLKN
                     ITASYQNTYMMNYARKTNPFPFSFQIGLPHTVPYDSLEAISNDNTLTLGSGITFSRRV
                     DSIINCSLMSNRRYASASNQTIGYTFPDITLSVMDIETLLGLGKYISGSRLNTGFQYT
                     VRQNGNLDWVKPKQESYTYALNPLLGFTGNLFKVVSTNLSFSLSQTKNITDMDTYEIL
                     KTSNTQSLNGNISYSFRAAKGFSVPFTKKKIHIKNELTSSLGITYENNFDKTKGSTTS
                     QVDRNTSRFAISPQATYQFDVNIRGGLTGSYEVNSDKKTEDGTSIFSLGVWVEINL"
     gene            complement(13149..13967)
                     /locus_tag="PLW08_04365"
     CDS             complement(13149..13967)
                     /locus_tag="PLW08_04365"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPL51869.1"
                     /translation="MRNLLLQEPVLVVDIGNTNIVCAIYLKGKSIWTVSLKSSRENTS
                     DEYYVLLGSLLESTTLPDGYKLGLKDIRYIVLGSVVPELTRVWKHLCSKYIQAEVYEI
                     NALSPLGISFRVDNPSFIGADLIANAYAAWQKYRTSSIIIDLGTATTIELISQEGMFE
                     GAIIAPGIKIGAESLFSKAAKLYEIELVSPTALLGLNTTEAMLSGIIYGHSFMLDTFI
                     AKIKEQYSQLKPFQTILTGGMSSLIKPYLSEINTIDKGLTLDGFYLALGKILNQ"
     gene            complement(14657..16837)
                     /locus_tag="PLW08_04370"
     CDS             complement(14657..16837)
                     /locus_tag="PLW08_04370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL51870.1"
                     /translation="MSRLRYFLIAIAVLLISILLPELPVISILLRTLDLSIYDTILEV
                     HNAITYSEYKGVYDQICIVDIDEKSIKELGQFSSWPSLFFADLVNILSADEPVAIAFD
                     VFFTESDSIQGYARERLRPEIEGLTANPDIIMDALSTDDSFAAAIENAGMVYLSMFNS
                     DYPSYTGALPDKLTAWKVQPKHFLQLKYPIPPIPQYANSARGIGFANIEPDVSGKIHD
                     YPLFLQYNSNAYLNFSMQICLDLLGVDQIKLNRNCQLLSGEKIVSELPLSPDGLFYFK
                     YYGQGGPDKTFRYISFSDVLFHRIPPGYFKDRLILVGTSAYGLKDSKITPLDNSFPGV
                     ELHSTFIRNVLEENYVRWVDSRILLIINILALLFMAIFIPRSKPFVSISVFLFISLLS
                     IPAVYLLYVKESYIYNYNYTLVPWVLGFLAMFITQAHEQGKEKRMVRDAFEHYVSPEV
                     IQEIMKEPEKLKAGGEKKYISIMYVDVRNFTSFCEQLTPSEITGFMNNYFNRATQTII
                     AHRGTLDKYIGDAILALFGAPVTYPGFEENAVKSALAIRQIVLNIKEEYKDHPVLKNF
                     RIGIGIATGEVIVGNIGSDRIFNYTGIGDKMNFGSRLEGLNKYYLTTIIIDEYTYRAV
                     SNTVFCRKLDKVKVKGKLNPCEIYEVIDTHENMQNQPELISAYHKYETALDLMLAGQK
                     NQAKTLFEEVLAILPSDEPSRIMLNRCEIIDWDTWDGSWQFESK"
     gene            complement(16852..17523)
                     /locus_tag="PLW08_04375"
     CDS             complement(16852..17523)
                     /locus_tag="PLW08_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51871.1"
                     /translation="MKKTMLIITFAGIALCLFSASVGKIRFLVGEVQYKQSLNLTYKP
                     ATLNMEVEPEGYIKTGPDSKAEIQWNNGSVAVINANTQISISKLQSDALANPNYTNKL
                     RERINNLRAQNEQREASQVAGIRREEAEIIPESELYWYSEPKYKVKDALNYMDIRDYT
                     KAVEIFEKVIEQSPLDKDAELSHTCLIMIYDELGDKVNLQKHIKQLKEDFPDSSALDS
                     LPPEK"
     gene            complement(17528..22099)
                     /locus_tag="PLW08_04380"
     CDS             complement(17528..22099)
                     /locus_tag="PLW08_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51872.1"
                     /translation="MKKSLFLIAIILISWVSLLAIPQHVHIVPVSTDKINSFGVDCFH
                     LQGSVAYCVDNTKGIIKAWDASKKEFLQPIFTKLPYAGKADDITGDETTLYILDSKAS
                     SIYLYGYDGNYLRTISFKGSPAVQFKKAIRILVNYQGFIYVLDAGRNELLSFTNEGMF
                     TGKISVMAPISMCLGEDQVIHILINKGRNQAMLHFDHNLTPCGSFTFSTPDSKADKIA
                     DSAINQYNEYYVIYALSTKIGKTDASGRLFPRSTWGSKDTNVSLVSFQQPTIIKTVDN
                     NGKVLLGILDSKVRTLKLYLDSEFSTMNVLQKPPFTIRPKLLENTAPKAKDYLVQDGR
                     KYYVHTTTVQYNKAKKTTDAITCKDDNSNIFNIYAITEGKRGVKGYDAIAVFNNKLYA
                     VDSKSCKVFIYNALTGDYMDSFGGKGNQNGRLNMPVSIAISSDGLIYIADRDNFRIAI
                     FNENSAHMLNLDFKSKNLKPQLLRISGQTLYFLANNSAIYKMSLDDDKNLKLLVSANR
                     ISTFDVLYENRIGYIDGLTQQLNIIYNNNTEHQYFTKNANGIFPGFAEIELIRYNPTD
                     NNLYICDKMAVNTRLLTFYFSPKKPQNIHFIVNQNKQAELSWDAAEGISNWLVIENNG
                     TQSVTYKVSEPRFVITEPKTQINTYKVQSISADGKSGPPSEEIEDAYSYYRYLSQNKN
                     YKEAAVALDRASKIFSGVNFKEELVQNYTLEAQMYISRNEFEKALSSIDSIEKITGVL
                     IDTAIWKAEIYRMMGDYKQGIAYLEKFKRNDDQNIMRELIALYYMDKNYAKVKELCNS
                     YLANFNRDKEVIRYLALAEEKLQNYANALSAMRELIAVEDNLDNNIKLGELQMLNKEY
                     ETAINSLLRTLQRFNNQGADVIQKLLGDCYFASGNYASAIDKYIDAIQLNPNEAEFYY
                     CLGNAYAKSRKAREATNNFAIAYQKMPAEVKYGFAYAQALDKEFRSTEALTVMDNIYQ
                     YIASDSTTTAYHEFYHDLLIKEQRYDDAWKEIQIALKYAPDNKLLQEKARTTSETRKY
                     YNETRDEIEIKKLEFYKVFPALCEYYKTNPIGTVTLYNTREISIENISVTVTIPQITD
                     RPYKKIIPALMGGEELTVDIIAPINNRIFDICKNGSATFNADLEVEWIFNKKQGAANK
                     SAIIQAQGINAMDWKDRKQYACFINPEDVNLRTFVNTHITQLFKTQPVGELNKNIQRA
                     VQVWCFYSANGIRYIPDMSTANLTGSEIDNVQFPFQTLTQKAGDCDDLLALLAATLSV
                     IGVECGFIDIPGHVMLVINTGITTEEIFSLGFEPSQFIYKNKKYWLPIETTLLGKETF
                     TASWKKASKDYNMLIEKGIDPQLIEFDIAHQLYPPAPYTEQISSYEYSNKTQAITKYE
                     TEIENIKLMGKVAQEEKFVETLKKYPTNLKVANQYALWCVKNNRPGKAAELWYQILTQ
                     DPQNLGALINLGNLQFNGGNYNEARINYLNALELSNDKDPILRNLCILEYKSGNQSQA
                     REYFNRMSNKNLLRDVNPTIYSDLLYIGE"
BASE COUNT         6907 a         4254 c         3840 g         7487 t
ORIGIN      
        1 gttggttacc gtagatatac caaacaccat tttctctttt aaaataggaa atatctccag
       61 agtcctccgg ttcatttaaa ataacggttt ccccatggga agtaaattta tttttactat
      121 gtacaacagc atagtcgtca tccaaatcaa tatagagaac ttctatttcc agcagggagt
      181 attgagccat tctatccagc catagatcat taaaattata acttgtcatt ccattatgca
      241 gatatttaat atgtagatgt tccattattc cacttatatt tttcaagttg aattccatac
      301 ttatatcata gagaatatcc cgaatttcac gctgggcaat tgtatcgctg tcattgcttt
      361 cgcagcttcc taaagccagc atcaataaaa taccgaagat tagatataag tatcttttca
      421 ttcttttcct aaccaattat tttattatct catatttata ttataatcgt tatatttaag
      481 aacggcaaga cattctatat gccaggtatt gggaaacata tcaaagggtt gtaaaaagac
      541 aactttatat ttctccccgc caattagtgt atttaaatct cgggcaagag tcataggagc
      601 acagcttaag taaataattt gtttaacttc cgcttgtata attgcttcca aagcgttttg
      661 ttgaactccg ctgcggggag ggtccattat aattgtatcg ggcttttcct ttattaaaac
      721 agcagacagg ttatcttcca ctttgcctac gacaaaagaa acattgttta gcccgttcag
      781 aactgcattt tcttttgcat cttcaattgc ctgaggaaat tcttccagca gaataacgcg
      841 tttaacctta tctgctaaag aaagtcctat agctccaatt ccgcagtagg catccagaat
      901 aacatcgtta ctattaacaa aatcctgcag gaaggaaata atcatttcca ttgttccggt
      961 atttatttgc cagaaactgc ggtagttaat cctgaatttc aaaccgcata aattatcgta
     1021 taaattctcg cttccgtaga gtagcttggt ttctttacct aaaataacat ttcctttttg
     1081 gcgattgata ttctggataa taccttttat ttggggaaat tcggtagtca gctttttcac
     1141 cagaagattg gtaaaaggca gttttccgct taaagtaaca aggatcacca aaatttgtct
     1201 gctatcagaa ttacagcgaa aaccgatatg tcttaattga cctttgtgtt caatttcgtt
     1261 aaagggctca accttcgctt taaggcaaaa ttccattagg gaattagcca gctgatcaaa
     1321 aatcggggga tgattttcac aagtatcgtg ttctactatt tgatgggaaa aacgggcaaa
     1381 cattccataa gtgattttac ccgtttgata atttttccct actggcataa agaccttatt
     1441 ccgataattt tttatttggg tagaaggagc aggaaagtaa ataacatcct tatctataaa
     1501 aggactgaag agttccagaa gcaactgatt tttttgtttc agctgttcag aatattttaa
     1561 catcagccaa tcacaaccac cacagggaat ttctgcagca aaggatgagc attggacctc
     1621 aacccgggaa tccgaataac ttatatattt tgtcactttc ccgtaagcat aatccttctt
     1681 ttctattgta atatcaatat ctacaacatc tccaacagca gtatagggga caaaaattgc
     1741 ctttccttct gcataagcaa tgccaaaacc gcccattgcc attttttcaa tttctaaatt
     1801 cagtatgtgt ttcaaagcat ttcctgaaga tgttgaatgc gcacttcaat aggagggtga
     1861 gtagcaaaaa gggaaatcct tttctgatta ttaatttttg ccaaggcata agaatcactt
     1921 cgtttttccg gaacataagc attagctatt tttttcaacg cttcaatcat aggacctgca
     1981 cctaccaaat ttgccgaacc cgcatcagct ttaaattcac gccaacgcga atatgccgca
     2041 atgggaatat tggcaagaag cattaaaatg ttttgcagaa ccataatcac cattatatat
     2101 ccaaaataac ctaatccgcc accgcgatca tcacgcaatg cattatcaat tacgaccgtt
     2161 aaaattctgg caaaaaacat cacaaaggta ttcacaactc ccatcagcaa agtcattgta
     2221 accatatctc cattggtgat atgagtcatt tcatgccccg ctacagcggc taattcctct
     2281 tccgaaaggc ggtctattaa agccgaggaa aaagcaatca aagcattgtt ttgggaagct
     2341 ccagtagcaa aagcattggc atccctgcta ttataaatac caacttccgg cattttaata
     2401 tttctttctt ctgccatact ttctattgca gaaagcaaat aagcaatttt gggatgcgca
     2461 attcccggtt ttacaatttg aattttatag cttgcttttg ccattggctt gcttaaaaac
     2521 agcgaaatta acgaaccccc cattccaaaa atagcacata taatcagcaa accccatagg
     2581 tcttccattt tcaaacccag aagacctact acaattccta acaccaacat taccagaatg
     2641 ttggtgagca ggaagattcc cattcgtttc agaaaattca ttttacgctc ctttatttat
     2701 ttttatcttc taaaattata ccttcaacca ataagaatat acttatatct tttccattgc
     2761 cattattatt tgtaggcaag gtaaggcaag aatttttttt aagatctgcg aactctttgg
     2821 caaagaaaga atttgccaac ctatcttttc tgaactgtat tatttaggat aagataactg
     2881 ccttttaatc ttcttcctta atagcattgc ttacctattg cttacccatt gctaatggca
     2941 ttagtattgg gtgagcaatg ctaaagcaat tctattagaa aataacttcc tgtcttgaca
     3001 gaattctgct tatttaaaaa aggggtttta tcaggaaaac atcaaatgaa acacagtaaa
     3061 attgaaattc tggaaaagac acctgttgcc tattctatac tttatcttgc cctacccagt
     3121 atgctaagta tgctggtaaa tatcttatat aatttaacag atactttttt tatcggcaag
     3181 ctgaatgacc cttttaaggt tgccggtgtc tctattgctc tgccttattt taatatgcta
     3241 atggcgattg cgggaatttt tgccaacggt ggagccagct atctctcccg tcttttaggg
     3301 aaaaaagatc taaaaacagc tcgggaaacc actactactg ctatttttac cgtagccatt
     3361 gtatcaatat ttgctgctac cttgggcgtt ctttttattc ctacctatct taatttatct
     3421 ggtgccagtg ccttaaccgc actttctgcc aggaagtatt taactgcaat ttttattggc
     3481 agtccgatca taatgattaa atttacttta attcaactgc ttagagctga aggttcagct
     3541 aaagaagcaa tgttgggttt gtttatagga acgggagcaa atattattct tgacccgttg
     3601 ttcattttta cttttaagat ggatgtaacc ggagctgcaa tagctacagt tattggtcaa
     3661 ggtttagcta tgctttatta tatttcttat tatttaaaaa agaagagctt agccagtccc
     3721 ggaagaaagt atttacaccc gcgttggtct tgctataaag aaatatttgc aattggaatt
     3781 ccttcctctt taagccagat tatgatgagt atcggaaatg ccataagttt taagctggct
     3841 tctgcttaca ctgatcatac cattgctgcc ctgggagttg tttcccgggt tttttccata
     3901 gttatttttg cttttattgg cttatctatt ggtattcagc cgcttattgg ttttaattat
     3961 ggagcacaaa attatactcg gatgaaaaaa gtaattaaga tgtccagtat aatctgtttg
     4021 ggaatgtctg ctgtctttac cttgtttttt gctcttttcc ctggagaact aattgcggta
     4081 tttattaaag ataaagcaat tatgaaaatt ggaacacaga tactgaatgc ctatgttttt
     4141 gctattccgt ttgcgggttt gggaatgata ttaatggcta cacttcaagc aatgggaaag
     4201 gcaatacctg ctttcatcgt ctccttatcg cgtcagggaa ttgtctatat tccaatgtta
     4261 tatttgctga attatctttt ccaattcagc ggtttgatct atgcaatgcc tattgctgat
     4321 gtgataacta ctattctatc gttcacattt gtgtatcata taaccaaacg gctgaaaagt
     4381 tccccttgac acaaaaaccc ctttataaat aagagtttaa gaaatagaaa taataaaata
     4441 tatggaggaa aaaatgtcca cgaatctgaa atgcgactgc tcaaccgata atgaaatagt
     4501 agaagtggaa aaaaattatg gaggaaaaat gtctcaaaat ctgaaatacg gcaacattag
     4561 cagcagtgaa gcaatggaac tggaagaaaa atatggagct cataattatc atcctttgcc
     4621 cgtggtttta gcaaaaggag agggtgttta tgtttgggac cctgaaggaa atcgttatta
     4681 cgattttctt tctgcctatt cagcagtaaa tcaggggcat tgtcatccca aaattattca
     4741 ggcattgatt gatcaggcaa aagagcttac tttaacctca cgcgcttttt acaataataa
     4801 gctgggagaa tatgaaaaat atataaccga atattttggt tacgatatgg ttttacctat
     4861 gaattcaggt gctgaagcag ttgaaacggc aatgaaactt gcccgcaaat gggcatataa
     4921 tgtaaaagga gtagcttccg attcagctat tcttatattt gcagataata attttcatgg
     4981 cagaactata tctattattt ctgcttcttc cgatccggat tgttttggcg gttttggtcc
     5041 attcactcct ggaattgtgc gtattcctta cgatgatgcc aatgctttaa aagagtatct
     5101 ggaaaaaaac ggcaaaaatg tagctgcttt tattgttgaa ccaattcagg gtgaagcggg
     5161 tgtagttgtt cctaaagatg gttatttaaa agattgttat gacctttgcc atcaatataa
     5221 tgtgctgttt atagccgacg aaattcaaag cggtttaggc agaaccggaa aacttcttgc
     5281 ttgtgattat gaaaatgtgc gacccgatgt tttaatttta ggtaaagcac tttccggagg
     5341 tgtattacct gtttcagctg ttttggcaga taaggatatt atgctgcaaa taaaacctgg
     5401 agaacatggt tccacttacg gcggttttcc tcttgcctgt gttgtagcta ttgctgccct
     5461 ggaagttatt aaaaaagaga aattgactga acgctctttt gaattgggtg aatatttccg
     5521 caatgaacta cgtaaaatag aacatcctat gctcaagctt gtgcgtggaa aaggtcttct
     5581 gaatgcaatt gtaattgagc caatgaatgg atttgaagca tgggatgtgt gtttaaaact
     5641 gaaagataaa ggtttgcttt gcaagccgac tcaccgtcat atcattcgtc ttgctccacc
     5701 cttgattatt accaaagagc aattggatga atgtattcaa attatcaaag atgtttttaa
     5761 tgatttaagt gcaaacttag gtcaataaat aactataagg ggtggctttt ccaccctttg
     5821 ttttttttaa tttatgaata attcagcaaa tttcaaaagc ggctttgtaa ctatcatcgg
     5881 taaacccaat accggtaaat caactttaat gaaccttatt ttgggggaaa aaatttctat
     5941 tacttctccc aagccacaaa ccacccgtta tgctattaaa ggaatatgga atacttctga
     6001 acatcagatt atttttgtag atacacccgg ctatttgaaa ccccgctatg aactgcaaga
     6061 aaagatgctt aaaatttggc ataatgcttt aaaagatgtg gatttgatta ttttcttaac
     6121 ccaaattgac ggttttccca cagaatatga caaagaagtg cttaaccagc tgaaaacttt
     6181 aaaaaatcct caattggctg ttttcaataa acttgacctc aatccggaag tagatagaaa
     6241 cgagttggta aaatatcttc ccgaatccat aaatgaggtc ttttttgttt ctgctaaaac
     6301 cggagagaac attccggaat tgatggaagc aattaaatac tatatccctt ttcacgagcc
     6361 ctattacgat aatgatatgc tttccgattt gccaatgcgt ttttttgctc aggaaattat
     6421 cagagaagca atcttccact tttttgaaga ggaaatcccc tattcttcag ctgttctgat
     6481 tgaacgcttt aaagaatttc ccgacaaggt aattattgat gctgtaattt ggctggaaag
     6541 agaaagccaa aaacctattc ttatcggaaa aaatggctcc tccatcaaaa aagtaaggga
     6601 atatgcagaa agagaactta cccgttttat gcaaatgccg gttcaagtac atttatttgt
     6661 taaaattaag cccaaatgga gaaaaaagca atcagcactg aaagaactcg gtttttagtg
     6721 tagccagagg agcagcatct tccggctata gattaatttc tacccaaaca cctaaactaa
     6781 aaatagaagt tccatcctca gtttttttat cggaatttac ttcatagctg ccggttaagc
     6841 cacctctaat attaacatca aactgataag ttgcctgagg tgaaatggca aaacggctgg
     6901 tatttctatc tacttgagag gtagtgctac ccttggtttt atcaaaatta ttttcgtagg
     6961 ttattcccaa agaagatgtc agttcatttt taatatgtat tttcttttta gtgaagggaa
     7021 cagaaaaacc ttttgctgct ctgaaactgt aggaaatatt tccgttcagt gattgtgtat
     7081 tgctggtttt cagaatttcg taagtatcca tatcggtaat gtttttggtt tggcttaaag
     7141 agaaactgag attggtagaa acgaccttaa aaagattacc cgtaaagccc agcaagggat
     7201 tcaaggcata agtataggac tcctgtttgg gcttaaccca atccagattt ccattttggc
     7261 ggacagtata ttgaaaaccg gtatttaatc gtgaaccgga aatgtatttt cctaaaccaa
     7321 gcagggtctc aatatccata acagataaag taatatcggg aaaagtgtag ccaatagttt
     7381 gattactggc actggcatat cttctgttag acatcagaga acagtttata atgctatcca
     7441 ctctacgaga aaatgttatt ccgctaccta aagtgagagt attatcatta gaaatggctt
     7501 ccagagaatc gtaaggaact gtatgtggta agccaatttg aaaagaaaaa ggaaaaggat
     7561 tggttttacg ggcataattc atcatataag tattctgata agaagcagtt atatttttaa
     7621 gcatagcaag agcattcaca agagtaatta caggattaaa acctggttct ttggtttctg
     7681 agggtttggt ggtttcagtt tccttctctt cttcttccgt cttttcttct ttttcacctt
     7741 tcccgaatat gtcttccata agtttattca cttcttcttc agaaaggtct gccagtttac
     7801 cttccgcttc taaacgagca agttcatctt tgcgtttttg gatttcttct tcgcttaatt
     7861 tctgttcttc ttcttttttc atctcttcct gtttcttttg ttccccttct tttttctttt
     7921 cctcttgttt tttttgttct tcctgttttt tcttatcttc ttcagtcatt tctgttttag
     7981 ctgaaccttc atcttcttta cctttgggag aaacactttc aggtggcagt ttacttttca
     8041 tcttcattgc ccaattggat aagagagacg aattcatcaa ggttaaattc atacgtatag
     8101 aacggtttgt attgccatct ctctgataag tatcaactgc ttgactttca acatattgag
     8161 tgtattttct ttgagtatcg gtatatcttg cagagacgga ggaagtaaaa ttaaacactt
     8221 gcggcaggta attgggacta taattcaaac ctaaatcctg aacatattcc gtttgtttac
     8281 caatgttgat atcataaaga tagtcctttt gcagtaaatc gcgttttgta ttaacccgtg
     8341 ccgtagcggt taaatcactt aacaaactcc aggagagatt attatcggta gtaatagttc
     8401 ttgtatccgt tgtctgagaa cgtttattcc aaaccaaact gtcaacggta gttagtcggt
     8461 cccagctttt aggttcgtta ctgctaaagg ttacactatt actgaaagta ttggggaaat
     8521 agctcaaacg gtagttctta aataatttga agcttgtttt ttcagcagga acattcaggt
     8581 tataattcaa agttcctcta tatgccaaaa tggtatccac tgcggtagaa gtattattat
     8641 aagagctttc aattcttccg ctaagggaag ttctatacac agtatattgc agaattttgt
     8701 ttttaggtgc ctgtttttgg ctgaaaccaa aatctgcgct gtaagccaaa cgttctgtct
     8761 tttcaatttc tttcagttca gggtctgcta tattagctct taaaaggtcg gaatttgctc
     8821 taaacctggg agttcctaaa gaataatttc gggtaagaga caaaggcata tccaaattcc
     8881 aggaattggg aaacagctta ttcaggaata ttttattcgt aatattgagg gattgagtgc
     8941 tggtaaagat attactgcgt ccgcgttgaa ttgtagtatt aaaattctcg cttttatcct
     9001 caaaatcaac atttatagtg gcaacatcag caaaagtgcc acttacacta aggcgtttgg
     9061 caattcctaa atcttcataa ggatcggcaa ctcggatatc attaaaatat ataattccgc
     9121 tgaattcttt tatatccatt tgatttgcat agaaatgaac tccgggaaca taaactccta
     9181 aatagatatc gcggatatta atcaagcttg gtttgccacg ataatatttt cccagttgtc
     9241 cttcatcgcc aggaattata ttaactaaag aatccgcttc cgcattagga ttaatttctt
     9301 tcagggaaat aaggtcttgc agattatatt ccagctgaat ccagtcgtta gcattcattt
     9361 tagcattata aggacgaacc ggaacccttt gcctaacttg ataatagttc aaggaatcag
     9421 ctccaatccg aaaaataata tagagcgaat ccaaattgga ataagaacct tgagccgctt
     9481 ccggataaac ccaaaaacgg attttatcgt aggaaagcag attttgagta tccagcatac
     9541 gctgtctgag taaacacatt tgatcccgtt gcaaatggtt cacttccaaa gttaaagccg
     9601 attctgccga ttcttttttt tcttcagtat accaggttcc agcaggtgaa gtatagtgag
     9661 taaagttctt ctgattagtt acaattccgg aaatatagct tgtatttttc gctatcaaaa
     9721 cagaaggttc aattatagga ttataatatt gaacatagct attagggggt ggcgcattac
     9781 catatttgaa ctggcggata aaattatcct gccatttatt accaacaacg gatatatctg
     9841 cgatgcggac ttttacatcc atctgggcat cggtatccaa ccaaattctg gcataagata
     9901 ttttttgtaa tgagggctga atattagttc cggaactgtt taccatttga tagtattgtt
     9961 catcattaag aggaatccga aacatccgcc actgattgta agaatccact accagagagg
    10021 aatccggact cgtaagcgaa aaagaataac tgagatagcg gtctaaagtg tttaaaactc
    10081 cattaccatc aaggtcttca gtatccaaaa ttctgttttc ttcagttcca ttcacacctg
    10141 aataatcatt gccacttgca ggataggcaa tgtcattagg atcgtgacct ggctgatttt
    10201 taggaattcc atccaaaccg atatcttcac tgattgtaag aactccatcg cgatttacat
    10261 cttcactgtt taaaacacca taaccaccat attcagtata aaaatcttcg ttaatatcgc
    10321 caaggtctat atgcatcgtc acattagggt aaatattagt attaggataa agaggttcca
    10381 gcttggcata aacctccaaa tatttctttt gcgaaaaatc aagttggttg cccagatatt
    10441 tcataattcc agcccagcta tgtgttgtgg aacctggaat ttgaacagga ttcggtttaa
    10501 cgatcatagt aagcacagtt acatattcat ccttttcctg ttccgttaaa gttgcttctg
    10561 cttcaatatc ttttcttttc acatctttgg gattgaacca gtttattctt cctttagcat
    10621 aagaggttga aaagggttta cttcccaaca tccaggtatt ataagtaacc cctaaagggt
    10681 aagaatccat aattgattcc atatcatcaa gatatgcttc ttttttcttg cttttgggat
    10741 caccataaat attgggcatt gtaaaagcaa cttcaccgga caaagaaagc tgggaaggag
    10801 ccgtagtatt aattaaaggc agggcatcca accatttagt aataaaagcc ggtttgaaag
    10861 ttaattcacc atcaacatct cccatcagca tttctatatt ttcattgcct attctggggc
    10921 gtctatcgga aacatcttca ctgcgataaa ttagagttcc tcccagtttt gcataatcac
    10981 tcaatttcca gtttgccctt atcccggcaa gatttttctg tgccacacta aagagggtgc
    11041 gggattcaaa ttcaatttca attttagcat cgggatcttt tccggcagca gttaaaaatg
    11101 ttatcctgcc aaaatcgtaa tccactatat aatcaatatt ttctctttgt tcattgccgt
    11161 ttactttaac ttttacactt cctttaagca cacccattga agaaagctca attgcttccc
    11221 gaccaatttt acctttaata ttaataaaaa tgcttgtgga gtcctgatag gaatattcat
    11281 cctcattttc tttgcggtaa attatgccat ctcccaaacc tctcattggc tcaataaaag
    11341 gcataataat ccagccattg gtaagattaa cggtagcatc atctccattg ataagtccat
    11401 cgggactgcc attagcattt aatcttaagt agtcattata ggtagaaata tcaccaaaga
    11461 ccagattatc gggtaaatta taatttctgg ttaagtccac attaacagta taaacctgca
    11521 attcaaaacc atcattttta atgttggttc tacccatatc gtatatattg cgcatttgta
    11581 aatgccaggt attgagttca tcattaggat tgtatggaat atattcctga tatttctttc
    11641 ttagaacttt cacatgtaag cgactggtat cagaattggg aggaggaact tgaactccat
    11701 ccctgcgagt atattgaacc cccaaagtgg cagtccgatc tatagttttc aagatagtaa
    11761 taattccgct gtcataatcg gttacaaaat ccgttccttc aattaattca tcataccacg
    11821 gttcataagg aggtctcata ttattggaat agtagatagt atctcctact gcggaagcgg
    11881 tattattggt ataattggca tcatcataaa acaaacgcac agttccattt gccggcaaaa
    11941 aatttacatt taccatccag gaaccgtctg gagcggtttc aattgcatta tttatccaac
    12001 ctggaggtat attagttccg gttttaagtt ggtaaagttg atagggatcg tgcaaataat
    12061 acattgttct tctggcatat ttccagctgc tgaccgtgga agaatccgcc tgacttttac
    12121 ctatgtaggt ctgagtattt ttttggcttt cttctttact ggcaattaca cttaagtcca
    12181 aattgccata tttgaattta ctggttatac caaaaagtcc ctgtgaactg gcagaatagc
    12241 ttacatagcg cgatccgctt aaagaaagag caatatttcc cccttcaata ctttggataa
    12301 attcgtcatc atagcccgta tattttacat ttacattgtt catatcaaag agttcttcat
    12361 cctgcttgga attgtattta aagtttacag caattttatc accgatagtt ccggaaagac
    12421 gaaggttggt ttccatttcc attttcaggt caaacacatt tttccctgat tgatcatata
    12481 tgggaacctg ttttctatgg gtattactaa cctgcatagt aagtttttga gttccatcca
    12541 gatttaagcg tcctgcagaa gaaccaagaa ccttttgcat cgctttcggc atagcaattt
    12601 ccggcagtaa agtaaggtct ttaaataaac cgctggtaac tgtctgcact acttgttctt
    12661 gggtcttgaa attagcaatc agggatttat gaaaaacctt tgtttgcata ttgctgaaat
    12721 aacggtcaaa actaatcgga acatcgggtg caattttata attacctact tgaacgctta
    12781 aaattacttt tccattggca aaatcaattt cttccttata ttctggtgca aaggcaggaa
    12841 tttcataggg ctttgtcttg taataattca tacctttgtt tatcttataa acttctatcc
    12901 gattgttaat aataggattg tattcagtaa gataaagacg ggggtataat gctttttctg
    12961 ttaagggatc aagctcgtgt tttatttcct ttttgggtct gaaatatctg gcaggaaaaa
    13021 tgcctttttg ctctgtttga tcgtcttccg gtggattaga accatcagga accgtctctg
    13081 tagcccacaa tatagttaaa gctaataaaa tcaaacaggc gaaaagcttt ttcgtcataa
    13141 attcaggatt attgattcaa aattttgcct aaagccagat aaaacccgtc cagagtaaga
    13201 cctttatcaa tagtattaat ttcactaagg tagggtttta tcaaagaaga cataccaccg
    13261 gtaaggattg tttgaaatgg ttttagttgt gaatattgct ctttgatttt agcaatgaat
    13321 gtatccagca taaaagaatg accgtaaatt attcctgaaa gcattgcttc cgtagtattc
    13381 aaacccagaa gagccgtagg agaaacaagc tcaatttcat atagttttgc tgctttactg
    13441 aataaacttt cagctccaat ttttattccg ggagcaatta tagcaccttc aaacattcct
    13501 tcttggctga taagttcaat cgttgtggct gttcccaaat ctattatgat tgaagatgtc
    13561 cgatatttct gccaggcagc ataagcattg gcaattaaat ctgccccgat aaaagaggga
    13621 ttatccactc tgaaactgat tcctaaagga ctaagtgcat ttatttcata cacttctgcc
    13681 tgtatatatt tgctgcataa atgtttccaa acccttgtaa gctcgggaac tacactgcct
    13741 aaaacaatat agcgaatgtc ttttagcccg agtttataac catccggtag agttgtggac
    13801 tccagcaaac tgccgagcag aacataatat tcgtcagaag tattttcccg actggatttc
    13861 agacttaccg tccaaatgga tttacctttc aagtatatgg cacatacgat atttgtattt
    13921 cctatgtcca ctaccagaac cggctcttgc aataataaat tacgcataat ccctgctcaa
    13981 tatcatttcc tgtaaagata ataaatcata cacaaatagc catttttgtt ataattctta
    14041 aatagcacta ttctgtcaat agatttctgt atttgaattg ataagagaaa cttatcttaa
    14101 atccctaaga atattccgaa gataagttca ggaacagagt tttgttcatt tttttgagag
    14161 cttcaatttc gggaaatatt cataaaattt caattccgga agggcaatta tatttcagag
    14221 atattagtgt cggcaaaaaa aggataacga aaaagtattt agtcccttta tttattatat
    14281 aaaccccatc taatttaatg ctttagccct atattttgtc cataagcgca gaaatatgca
    14341 taattaattg ttatttagtc aattaagtaa cgacaccgta acacttccgt aacacttccg
    14401 tagttccgtt acggagttgt tacttgagtg ttactttatc ataacggggt aatgtatgta
    14461 taagaggaag gaaaataata ccggaaaaat cataaatgca atctttattt ttagaagggc
    14521 ttacgcaccc atcgctatca ttttatcgcc ctgacgggtt ttgaggaatc taaacctttc
    14581 aaatcctctc aatcctttca accaatttta ccaatcaaat ctgtgctatc cgttttatct
    14641 gtgagagctt attgttttat ttactttcaa attgccaact gccatcccaa gtatcccaat
    14701 caataatttc gcaacgattc aacataattc tactgggttc atccgagggt aaaatggcta
    14761 aaacctcttc aaaaagggtt tttgcttggt ttttttgtcc agcaagcatt aaatctaaag
    14821 cggtttcata tttatgatac gcagaaatta actccggttg attttgcata ttttcatgtg
    14881 tatcaataac ttcataaatt tcacagggat ttaatttacc cttcactttc accttatcca
    14941 gtttacgaca aaagacggta ttgctaactg cccgataagt gtattcatca ataataatag
    15001 tggtaagata atatttattc aatccttcca ggcgggaacc gaaattcatt ttatcgccaa
    15061 tgccggtata attgaaaatt ctatcggagc caatatttcc tacaattact tctcctgtag
    15121 caataccaat gccgatacgg aaatttttca gaacaggatg gtctttatat tcctctttga
    15181 tattaagaac aatttggcgt atagcaagag cgctttttac ggcattttct tcaaaaccgg
    15241 gataagttac gggagctcca aacaaagcta aaatagcatc tcctatatat ttatccagag
    15301 ttccgcgatg agcaatgatg gtctgtgttg cccgattgaa atagttattc ataaatcccg
    15361 ttatttcaga aggagttagc tgttcgcaaa aagaagtaaa attgcgcaca tcaacataca
    15421 taatactgat atacttcttt tctccacctg ctttcaattt ttccggttcc ttcataattt
    15481 cctggataac ttccggtgaa acataatgct caaaagcatc ccgcaccatt cttttttctt
    15541 taccctgctc atgtgcttga gtaataaaca ttgctaaaaa gcccaaaacc caaggaacaa
    15601 gagtgtaatt ataattatat atataactct ctttcacata aagcaaataa acggcaggaa
    15661 tagaaagcag agaaataaat aaaaaaacgc taatagatac aaatggctta ctgcgaggaa
    15721 taaaaatagc cataaagagc aaagccaaaa tattaattat tagtaaaata cgggaatcca
    15781 cccaacgcac ataattttct tccagcacat tcctgataaa tgttgaatgt aattccacac
    15841 ctggaaagga gttgtccaaa ggtgttattt tactgtcctt caatccgtag gcagaagtcc
    15901 ctactaaaat aagccggtct ttaaaatagc caggaggaat tctatgaaaa aggacatcgg
    15961 aaaaggaaat ataacgaaat gttttatcag gacccccttg tccataatat ttaaaataaa
    16021 acaaaccatc gggactaaga ggcaattcac tcacaatttt ttctccggaa agcagctgac
    16081 aattgcggtt caactttatt tgatcaacac ctaataaatc taagcaaatc tgcatactga
    16141 aattcagata agcattactg ttatattgca aaaaaagggg ataatcgtga atttttccgg
    16201 aaacatcagg ttcaatattg gcaaaaccaa ttcctcttgc tgaatttgca tattgaggaa
    16261 tgggaggtat aggatatttt aactgtaaaa aatgtttggg ctgaactttc caggcagtta
    16321 atttatcggg aagagctccc gtataagaag gataatcact gttgaacata gataaataaa
    16381 ccattccggc attttctata gctgcggcaa aactatcatc agtacttaaa gcatccataa
    16441 ttatatccgg atttgccgtc agaccttcaa tttcaggacg taacctttct ctggcatatc
    16501 cctgaatgct gtcgctttcc gtaaagaaaa catcaaaagc aatagccaca ggttcatctg
    16561 cagataaaat attaactaaa tcggcaaaga acaaactggg ccaggaagaa aactgtccta
    16621 attccttgat gcttttttca tctatatcca caatgcaaat ttggtcataa acacctttat
    16681 attcactgta ggtaatagca ttgtgaactt ccagaatagt atcataaata cttaaatcca
    16741 aagttcttaa taaaatactg ataactggca gttcaggcaa taaaattgaa ataagcaata
    16801 ctgctatagc aatcaggaaa taacgcaaac gggacatcaa ttagcttact tttatttttc
    16861 cggtggcaaa ctatccagtg cggaactatc cggaaaatcc tccttgagct gttttatatg
    16921 tttttgcaga ttaactttat ctcccagttc atcataaatc ataatcaaac aagtatggga
    16981 taattccgca tccttatcca gaggtgactg ttcaattact ttttcaaaaa tctccactgc
    17041 tttagtataa tctctaatat ccatataatt taaggcatct ttaaccttat atttcggttc
    17101 agaataccag tataattcgc tttccggaat aatttcagct tcttctcttc ggattcctgc
    17161 aacctgactg gcttcacgct gttcattttg agctctaaga ttattgattc tttcccttaa
    17221 cttatttgtg taattaggat tggctaaagc atccgattgg agtttactga tgctgatttg
    17281 agtgttggca ttaataaccg caacgctgcc attgttccat tgaatctcag ctttggaatc
    17341 gggacctgtt ttaatataac cctcaggttc cacttccata ttcaaggttg ctggcttata
    17401 ggtgagattt agtgattgtt tatattgcac ctcacctact aaaaaacgaa tcttacctac
    17461 agaagcagaa aaaagacata atgctattcc tgcaaaagtg ataataagca tagttttctt
    17521 cattttttta ctctcctata tatagcaaat cggaatatat tgtggggtta acatcacgta
    17581 gaaggttttt attgctcatc cgattaaaat attccctggc ttgactttgg tttccgcttt
    17641 tgtattccag aatacagagg ttgcgtaata ttggatcctt atcattacta agttctaagg
    17701 catttaaata gttgattctg gcttcgttat agttacctcc gttaaattgg agattgccca
    17761 aattgattaa agcaccaaga ttttgaggat cttgagttaa aatttgatac cataattctg
    17821 cagcttttcc gggacgatta tttttcacac accacagagc atattgattg gcaaccttta
    17881 gattggtagg atatttttta agcgtttcca caaatttctc ttcctgagct acctttccca
    17941 tcaattttat gttttcaatt tcagtttcgt atttagttat tgcctgcgtt ttattgctgt
    18001 attcataact ggaaatttgt tctgtgtatg gtgctggagg atatagctga tgtgcaatat
    18061 caaattcaat taactgtggg tctattcctt tttcaattag catattataa tccttgctgg
    18121 cttttttcca gctggcggta aaggtttctt tacccaaaag cgttgtttca ataggaagcc
    18181 agtatttttt atttttatag atgaactggc ttggttcaaa accaagagag aaaatctctt
    18241 ctgtagttat tccggtattg ataaccagca taacatgccc cggaatatct ataaaaccgc
    18301 attctacacc tattaccgat aaagtagcag ccaatagagc aagcaaatcg tcacaatcac
    18361 cagctttttg agttagggtc tgaaaaggaa attggacatt atctatttca ctgccggtca
    18421 aattagctgt agacatatcc ggaatatatc tgataccatt agcactataa aaacaccaaa
    18481 cctgaactgc gcgttggata tttttattaa gttctcccac gggttgagtt ttgaaaagtt
    18541 gagtgatatg agtatttacg aaagtgcgca aatttacatc ttcaggattg ataaaacagg
    18601 catactgttt acgatctttc cagtccatag cattgatacc ctgtgcctga ataatggcac
    18661 ttttattagc agcaccttgt tttttgttaa atatccattc aacctccaga tccgcattaa
    18721 aagtagcgga accatttttg cagatatcaa atatcctgtt gttgatagga gctataatat
    18781 ccacggtaag ttcttcacca cccattaaag ccggtataat tttcttataa ggacgatcgg
    18841 ttatttgggg aatggttaca gttacagaaa tattctcaat ggaaatttca cgcgtattat
    18901 aaagggtaac ggttccaata ggattggttt tataatattc gcagagggcg ggaaagacct
    18961 tgtaaaattc aagtttctta atttctatct catctcttgt ttcgttataa tatttccttg
    19021 tttcagaagt agttctggct ttttcctgta aaagcttatt atcaggtgca tatttaagag
    19081 caatttgaat ttctttccag gcatcatcat agcgttgctc tttaattagt aaatcatgat
    19141 agaattcgtg ataagcagtt gtggtgctat cagaagcaat atattgatag atattatcca
    19201 tcactgttaa agcttcagta gaacgaaatt ccttatccaa tgcctgagca taagcaaaac
    19261 cgtattttac ttcagcgggc attttttgat aagcaatagc aaaattattt gtagcttccc
    19321 tcgctttacg acttttggca taagcatttc ccagacaata ataaaattct gcttcgttag
    19381 gattgagctg aatggcatct atatatttgt caatggcact cgcataattt ccggatgcaa
    19441 aatagcaatc gccaagaagt ttttgaataa catccgcacc ctggttattg aatcgctgca
    19501 aagttcttaa taaagaattg attgcggtct catattcttt attcaacatt tgcagttcac
    19561 ccagtttaat attattatcc agattatctt ccacagcaat caattcccgc attgcagata
    19621 aagcattggc ataattctgc agtttttctt cagctaaggc aagatatcgg attacttctt
    19681 tatcgcggtt aaagttagct aaataggaat tgcagagttc cttcacttta gcataatttt
    19741 tatccatata atacaatgca atcagctcgc gcataatatt ttggtcatcg tttctcttaa
    19801 acttttccag ataagcaata ccctgcttat aatcccccat cattctgtat atttcagctt
    19861 tccagatggc agtatctatc agcactcccg taattttttc tatagagtca atgctggata
    19921 atgccttttc aaattcgttc cgactgatat acatttgagc ttccaaagta taattctgca
    19981 ctaattcttc tttaaaatta acacctgaaa agattttgga agctctatcc aatgctactg
    20041 ctgcctcttt ataattctta ttctgagata aataacggta ataagaataa gcatcttcaa
    20101 tttcttccga aggcggaccg ctttttccat cagcagaaat tgactgcact ttataagtat
    20161 ttatttgtgt tttcggttcg gtaattacaa aacgcggctc actaacttta tatgttacac
    20221 tttgagttcc attgttttca attactaacc aatttgatat cccttctgct gcatcccagg
    20281 aaagttctgc ctgtttattc tgatttacta taaaatgtat gttctggggt ttcttgggac
    20341 tgaagtaaaa agtaagtaat cgtgtattga cagccatttt atcacaaatg taaagattat
    20401 tatcagttgg attataacgg attaattcaa tttctgcaaa accggggaaa ataccgttag
    20461 catttttggt aaagtactga tgttctgtat tgttattata aataatgttc aactgctgtg
    20521 ttaatccgtc aatatagcca atcctgtttt cgtataaaac atcaaaagtg cttattctat
    20581 tagctgaaac taatagtttt aagtttttgt catcatccag ggacatctta taaatagccg
    20641 aattatttgc cagaaaatat agtgtttgac cactaatccg caataattga ggttttaaat
    20701 ttttactctt aaaatctaaa ttaagcatat gagcagaatt ttcattaaag atagcaatcc
    20761 gaaaattatc cctgtctgca atataaatta aaccatcgga agaaatggct atactaacag
    20821 gcatattcaa tcgtccattc tgatttcctt ttccgccaaa ggaatccata taatcacccg
    20881 ttaatgcatt ataaataaaa accttgcagg atttactatc taccgcatac aatttattat
    20941 taaagacagc aatagcatcg taacctttca ctcctctttt cccttcagtt atagcataaa
    21001 tattaaaaat gttgctgtta tcatctttac aggtaatggc atcggtggtc tttttagctt
    21061 tattatactg cactgtggtt gtatgaacat aatattttct gccgtcttga acaagataat
    21121 ccttggcttt cggtgcagta ttttccagca atttgggtcg tattgtaaaa ggaggttttt
    21181 gcaacacatt cattgtagaa aactcgctat ccagatagag tttaagagtt cggactttgc
    21241 tgtccaatat ccctaaaaga acttttccat tgttatctac tgttttgata atagttggtt
    21301 gttgaaaaga aaccagagat acattagtat cttttgatcc ccaggttgat cggggaaata
    21361 ggcgtccgga agcatctgtc ttgccaatct tggtactcag agcataaata acataatact
    21421 cattatattg attaatggca gaatcggcaa ttttatctgc tttgctatct ggagtagaaa
    21481 atgtaaagct accgcagggt gtaagattat gatcaaaatg cagcattgcc tgatttctgc
    21541 ctttgtttat cagaatatga attacctggt cttctcccaa acacatagat ataggtgcca
    21601 taaccgaaat tttacccgta aacatacctt cattggtaaa agaaagcaat tcatttcgtc
    21661 ctgcatccaa aacataaata aagccctgat aatttaccag aatgcgaata gcttttttaa
    21721 actgaacagc gggtgaaccc ttaaaagata tagttcttaa atagttgccg tcgtatccat
    21781 ataaataaat tgagctggct ttggaatcca gaatatataa agttgtttca tctcctgtaa
    21841 tatcatccgc cttaccagca taaggaagtt tggtaaatat cggttgcaaa aattctttct
    21901 tgctagcatc ccatgcttta attatgcctt ttgtattatc tacacaatat gcaacactac
    21961 cctgaagatg aaaacaatca actccaaagg aattaatctt atcagtagaa acaggaacta
    22021 tatgcacatg ctgaggaatt gctaatagcg atacccaact aatcaagata attgcaatca
    22081 ggaaaaggga ttttttcata ttaactccct gttttttcat aatctaaatt atgttatctt
    22141 cacacagcac caaatcttaa aaacctcctt tttcaagagg gctttaagcc gacaaatcca
    22201 tttattttac gcagagataa taaccagttc ttcaacttta tatcctccac ttttatcttt
    22261 tttcttttac ttcaaattat cttattctgt caaggaaaaa attatcctgc aaaagctcca
    22321 atttttttaa caatatattt ggaacgaagt taattgtaaa aaaaagcact ttttagcgga
    22381 taactcctaa ttgcatttct taagtatatt tgtaagcatt ttaacttgtc ttcaatcgca
    22441 ataatactga tgaattaaaa tgcataaaca ttttttctgt cttatatg
//
LOCUS       DAOOZV010000030        21923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2526_length_21923_cov_63.428571, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000030 DAOOZV010000000
VERSION     DAOOZV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_21923_cov_63.428571"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1917
                     /locus_tag="PLW08_04385"
     CDS             328..1917
                     /locus_tag="PLW08_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51873.1"
                     /translation="MKKYLFLVLILLPVILLGQAGYGEISIPGDPMWEEESETTEGTH
                     WGMGGAVGAVTIDNQTYSQIRLQPELQVWKFGLGLDIDLIIDAEGKVRKEDWDEWQDY
                     VNKILFLRFAQRNDPFYFKVGSIPDYTLGNGLIFNHYSNMTLYPQVKNVGGYVGINTH
                     LSGLGFEAYTHNLHKNEILAGRVHFQPLAVTNIPLLEKLRVGVNVGIDRDQYGKYEDK
                     DGDNIPDVYDKFPKDKNRYLDTDNDGIADNDDIDINGNNILDHPFVNPYVAEHFPGIE
                     NLGYVLDMDVTPDSAAVYNEKDEVLVYSLDYQLPLIESERFNLIHYGEIAKIDGYGTG
                     MIFPGFSSKFFIFDARLEFRNFGDQFLPGYFDRQYDQQRAQVRHIISDGNGNQIWYLT
                     TKEETLKDVESSLGWFGYLRANLFNIVYAKIAYQDMYGKQNFLGKSLWASVTVCPSIV
                     PKLKEASVCYAQTNVNYINFKYPRNDNANILGRLVYSISDNANLVGSYREYYTDINND
                     GKIKGKDEIIETFTFGVEFWF"
     gene            complement(2317..3483)
                     /gene="manA"
                     /locus_tag="PLW08_04390"
     CDS             complement(2317..3483)
                     /gene="manA"
                     /locus_tag="PLW08_04390"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPL51874.1"
                     /translation="MPYLIKPSLQNYSWGDTKYLQELIGEQQEIGKPLAEIWFGAHPK
                     APSLVITEKGEIPMDKLLQDMPENMLGTEQNIALPFLLKILAAAEPLSLQVHPDKQTA
                     QNGFRKEEQEGIPLNDPKRSFKDPNHKPELICALTPFIAMCGFRPFDEIIRNFHILGI
                     IEIWQKFSAFNKNPSKNTLRDLFSQILESKSSQLEEVMHCLEIIQPKLSPKLKFTCQI
                     CKHLNEFYPLDSGIVSPLLLNTFTLQPGQAIFLDAGILHSYIQGAGIEIMANSDNVIR
                     GGLTPKYINREILFAITRFETYLPQLIHPHMKINELVSYPTPAEEFYLSRLILEGTYI
                     LDNLHKPLLVFCFKGSFTTEDGFSLERGKAMFISFAERPVTLSGSADLFIASIP"
     gene            complement(3495..4958)
                     /locus_tag="PLW08_04395"
     CDS             complement(3495..4958)
                     /locus_tag="PLW08_04395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPL51875.1"
                     /translation="MPPLIQNILNSLVYDPQKPLLFHSTFFLFFFIVVLLFYPLVVNK
                     VKVRTWYLMLASLYFYYKTSGSFIILLLITSLINFGLGAFLYNAQSKATKRFYLWLSV
                     FWNLGSLGYFKYTNFIITTINQVFGGQLPLYDIFLPVGISFFTFQTWSYTLDIYFGKL
                     KPLKSFVDFTFFVSFFPQLVAGPIVRASYFIPQIHKKLSLDKDTIAKALILIFSGLIK
                     KGVIADYLSVNFVDRIFENPALFSGLENLLGVYAYALQIYCDFSGYSDIAIGLAALLG
                     FSLPVNFLTPYRASSVTDFWRRWHISLSTWLRDYLYIPLGGNRKGKARQYLNLMITML
                     LGGLWHGASWNFVVWGGIHGLALVLDKMLLRLKIMQTKVMKVIGVIVTFHFVCFGWIF
                     FRSPDFFSAGMMLNRIFTAFNGSIFWQWLTQYKICAILIITGFVFHWMPDSYNNALQK
                     TLSKAPVILQSLILAIVIWILFQFRSAEIQPFIYFQF"
     gene            complement(4971..6269)
                     /locus_tag="PLW08_04400"
     CDS             complement(4971..6269)
                     /locus_tag="PLW08_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPL51876.1"
                     /translation="MHIRRLLPFCILALVCIFPVSKSYAEENITTEDINELFSEEEYP
                     SDMDTLEEFIQSYYLLLESNEYLKQYKFLNLDKNQFLGDKKALKPFYEKLLELRSGLR
                     QTVTIYQIGDSHIQAGYFSGTARTSLQQYFGNAGRGLVFPLRLAGTNQPDDYRISASG
                     SFTRLNSERGLSGYALKMADGLCLEIATNNFFRGDNSFNKLSLLTSLPGMETKLEFAS
                     AEPEKNAEKIGNYYYSRVYWETEVSKVKLTLTGESSTLYGICLEKEQPGLLYHSCGIN
                     GAGFYNLLEQHILFEQIGLLNPDLIVISLGTNDAQGTFRGEVFTKNLNTFMYKLRQFN
                     PETPVLFTLPPDSNKRGKHNSDLDKVDKIIIDYAKENQCAWWNLNEVMGGNNSIQKWR
                     NQQMSAKDLLHFTPKGYMLQGYLFYQAFIKSYKSFSEQSG"
     gene            complement(6270..7661)
                     /locus_tag="PLW08_04405"
     CDS             complement(6270..7661)
                     /locus_tag="PLW08_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51877.1"
                     /translation="MPKNFIRPLIIVVLAVILVEILARVIPAVDNHFFPLKKLSWFES
                     LKREKAVVSADTSFVQKAVAISNDLLPLKQFITKLNNPSENLRIAYYGDSIIEGDLIT
                     GKLRMELQSMHQGSGVGMVGITSIVAGFRQTIKHSFSRNWESISFMTPHKYDIPLGIT
                     GYTFIPRNYYVAEKVIEPLQLDSLAVLDSAQVASPEPQKESARFYVDYDPWVEYSASQ
                     VAGGASTFNRIRLFYSHSSDSSYVKVSYNGAPFILRHLQNQPGVQVLDLSPVTACKKI
                     RLEFNAYDPIHLYGVSFDEPQGAYVDNYPIRGYSGMYFQRISSDVLSGFQKHLHYDLI
                     ILQYGVNVSNYQIRDYSYYAKGMKKSIEHLQKALPGVPILLISAHDRCIKQGTEYVTS
                     PDIPYLVSTQAKIAKETGCGFWNLFSAMGGLNSMPKYVNHQPPLAGKDYTHFTRAGAD
                     HIAEMLLAYLKGN"
     assembly_gap    7745..7844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7940..8341
                     /locus_tag="PLW08_04410"
     CDS             7940..8341
                     /locus_tag="PLW08_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPL51878.1"
                     /translation="MKKTKFGLLLGIGICAIIIVSCSAPETYSSKPVSQSTEQKTPEI
                     EKVSKVVNYHQDKLQAIYKKHTYQNAMQGDLEFTLYITNEGKVQEVEVKALSGKFFSN
                     FIDELKKEIFTWAFPKQDKIIYSFVVSFRKG"
     gene            8546..9610
                     /gene="prfB"
                     /locus_tag="PLW08_04415"
     CDS             8546..9610
                     /gene="prfB"
                     /locus_tag="PLW08_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPL51879.1"
                     /translation="MKFGGYFDPEPKKHLIMELEQKMNAPDFWEDQTQAKKISKQLAQ
                     LRDELEHLKKLDTVKSELEIYLTLLDEDFNAELLAEAVSELPRIQSFIEKAEIECYLN
                     DKYDHNNALLTIHSGAGGTESQDWAEMLLRMYAHWAEKNDYSFDIIDSLPGEEAGLKS
                     VSVEIKGDYAYGMLKSEIGVHRLVRISPFNAQGKRQTSFASVFVYPEFDEDLEIEINP
                     KDLKIDTFRASGAGGQYVNTTDSAVRITHLPTNITVTCQNERSQMQNREKAMSILKSR
                     LYQYYEEQREKEKKNLENSKTEIGWGNQIRSYVFHPYQMIKDHRTNYETGNVDKVMNG
                     DLNDFIYAWLKYNAHQRING"
     gene            9603..10343
                     /locus_tag="PLW08_04420"
     CDS             9603..10343
                     /locus_tag="PLW08_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPL51880.1"
                     /translation="MAKQDYKELLNELDKNKLPVHIGFIMDGNGRWAKKRNRPHLYGH
                     RAGVKALRQVVELGVELKLQYLTFYAFSTENWSRPESEVKGLLRLLKEQLKKQIPELM
                     EQNIYAQFIGSTVGLEENYWKEVSSLVAQTHNNTGMIVNFAFNYGGRLEIIEGFKKFM
                     TMHPEEWHKLTPEEFGNYLWTAGQPDPDLIIRTSGEKRLSNFLLWQSAYAELYITDTL
                     WPDFDKVELIKALLDYTSRERRFGGRNE"
     gene            10336..11136
                     /locus_tag="PLW08_04425"
     CDS             10336..11136
                     /locus_tag="PLW08_04425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPL51881.1"
                     /translation="MSELAKRVLVAVILIPIVLIVLYYRGLPLVITLLIITFLSSWEY
                     AQMLNKAGIKIHYVWLAFNSLLYLAFVYMKGKDLSLLWLVLFLAIGEAILTWEKELSV
                     PRIFAVLFGFVYTALFPALIARLGLYYAEHNFLLALILLIWIVDSVAYFAGIKIGKHR
                     NITAISPQKSLEGFIAGVLAPWLILIILYICKVEILPFSYLALLAVASGIFGQIGDLA
                     ESMLKRFCKVKNSSNLLPGHGGILDRCDSCLFAGSFLYCALEILTKVR"
     gene            11142..12881
                     /locus_tag="PLW08_04430"
     CDS             11142..12881
                     /locus_tag="PLW08_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPL51882.1"
                     /translation="MKKALTLIVMLAATAAMFAQVYAVDLFFSEYVEGTSNNKALEIF
                     NGTGEPVDLSNYTVKLASNGGTWSSTNILNMSGVLDDGDVYVIANSQAEASILAIANV
                     TSTVTYYNGNDVVGLFNGNTLIDIIGVYQQDPETANWPVAGTPSATAEHTLIRKPTVI
                     QGNTDFLAGAGTNADDSEWIVYPQNYFADLGQHTFNPGAPEQAATPTFNPPAGVYANP
                     ISVTISSTTPGATIRYTTDGTEPNETSTLYSAPIALATTTTLKAKAYATGFSPSYTAT
                     ANYIFPVQVSNIAALRASAADGTTIYQLANEVILTMQQNWRHQKFIQDATAAILIDDY
                     NNIMNTPYNIGDGITGITGTLNRYTTGMLQFWPVINPGPATSTGNVIIPQLVTIAEIT
                     NNAELYQSRLMRLDNVHYASPSGNYAAQTSYDLLDATGTIVMRTQFSDADYIGTPMHQ
                     GNFNVYVIVTQYNATTQVTPRMLSDFNPVANDDNVLTPMQIELIGNYPNPFNPETTIR
                     FKMEKAAPAEVIIYNDKGQIVKTFSTIATQGINSVVWDGKDNNGHPVASGVYLFRLKS
                     GSYSSTKKMVLMK"
     gene            13018..14214
                     /locus_tag="PLW08_04435"
     CDS             13018..14214
                     /locus_tag="PLW08_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL51883.1"
                     /translation="MLFYNLEAMPISKRKLLLITDLYPNKFNPVNGVFVQQQAIELSR
                     YYQVQVVAACDKNPYLVYEHHQDGFPVKLICYPYWQKYFLTSLITYRILALPAIKRCY
                     KRFQPDIIHIHDYRHIPELFWLKPWLNKLSVPKYLTLHNIRTHPERLKNNPRIGFYRW
                     ALKKTLTGWKHIFTVNSKIAKWVKQYNPASKVTIIGNAISELAEVDPQLIEPYKNILL
                     ADYFHLISIGNLFTEKGFSYLIEAISLLKKEGLQVQLIIAGDGDRRKELENQVRNNNL
                     NEEVIFLGRIENKLLRNILPLFDLFVLASYSETFGIVFLEAMFAGLPVIGIKEEGIYN
                     LAEDGKQALFAEPKNSKDLAAKIKLLMMNPLLREEIAKAGQRLVQEKYMLKELIKRII
                     AVYEQK"
     gene            14201..15490
                     /locus_tag="PLW08_04440"
     CDS             14201..15490
                     /locus_tag="PLW08_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51884.1"
                     /translation="MNKNKILMIINEFPPTGQSGVQRPLKFVKYAVKAGWEVHIIAPK
                     KPVRKVVDYSLLQEIPKEAHIYRVAGLGIKTPDESKMVNAHFAETAPPSKIERAFWRL
                     AKLVNDFLFPYDKQIGWMPFAYYTACKIIKKQQIRNIYITAFPYSAFLVGLALKKKYK
                     DKIFWVADYRDSWQFGIMIEKLVFPFRLKTIRKTDDKVLATCDAAVFVTPETQLEYIN
                     KHSWLKEKSYYIPNGYDEDDFTGITPKKFDKLTLVLMGKLTKVYGSPLNLLKALEDCF
                     PNNYQVIHIGNIDKAILHNIKTSGYTSYNYLGYQKHSDAIAYSLGADINLIILNENST
                     AKYWYPGKLFELLRCGKPILALGPQESNLEKILSATKRGRYAYINDKEQIKAQIEYIL
                     KNPDQFDTSPETIKQYSREELCKQLLAIYEKGQSSNS"
     gene            complement(15472..16518)
                     /locus_tag="PLW08_04445"
     CDS             complement(15472..16518)
                     /locus_tag="PLW08_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL51885.1"
                     /translation="MKKVTQGKVLLIHTWGIGDLILLTPVLQAVKTLYPLLELEILFF
                     PKSAAIPVTNAPFISKIHFSGWKANLLLPTLFKLRKEKYQAVLFSSGVRPWKTWLFML
                     LLKAEIKVGEYKKFRYPGLSVYVKFNPLLSRTRSNYTLFQSFLNLPSWEEAIVRQNEL
                     NLYPYFHLTEANHQWAENFLAENNLTGKKIIGIHPGCMAKNKRRRWNKEYFIALIEKL
                     KRQYDYPILIIAGPDEIEVGKAIQARTKNLLLLNAPLANVAAIIARLHFFINTDSGLG
                     HIASCFGIKGLTIFGPANELQTAPFSNNSNIIRYPVDCAPCIDKKKKPNCTLDCLLKL
                     TPDMVFKRVQELLD"
     gene            complement(16529..17872)
                     /locus_tag="PLW08_04450"
     CDS             complement(16529..17872)
                     /locus_tag="PLW08_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPL51886.1"
                     /translation="MSQINLKKNIVTSGSFRIVILILSFLISWISARYLGITLKGEYS
                     YLITLGGFIWMILDWGIFRSYPYLVRKYPDEVKTMFGWTILTMFLELLIFSCLGIVFL
                     DFWSKLIGYPLTPAKMILLILFISFNKAFMQLQAIYMGMDRILENSLAHFLSSAIFFV
                     LLLIGYFALLEVDRINAVMVFTVAGLISSIIYLVLRTKWTNWYKQLNIKLVKLFYSYG
                     FRVFLSSLFIMLLIRADIVIIKHFLDFKEVGIYSMAAHIVDFIQIASNLVGGLLFVKL
                     SDIDDDISKWLLLKKTLLLFFFFITLANLGFGLLGKFIMRIMFGVQFVPVYYVYFWLM
                     PAVYGLSFGSLFNNYLNSKGFPIISIILPAVALVINIGLNLWLIPVMGINGAALSSSF
                     SYFLWFILIVYFEQKDSQGKMLKYLLPTRKDIIDLWQETMADLKGLWEKFLHKTA"
     gene            18408..19556
                     /locus_tag="PLW08_04455"
     CDS             18408..19556
                     /locus_tag="PLW08_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL51887.1"
                     /translation="MVIAKIVFWISWLLLAYHFVGYGLILFIITSLFKKAKISIPAPD
                     VFPSITVICPAYNEEKVIEEKIRSFLQLNYPKDRIKMIIISDDSTDKTNEIVQKYTDQ
                     NISLVIQKPRAGKQNAHNLVLPLLDTDYVLSTDANSIFTPDCVQLLVAKMLSDKRIGL
                     VSGEVKMVKRGSKQSGEAHYWKYEAFLKLMDSRLKTLIGANGPLYLIKRELFSEIPPN
                     SPDDFERVLLTLKRRYIAAYEPQAIIYEEVTEKATEEISRKVRIITQEWCVLKRNIEL
                     LNPFRYPAVSFILFSHKVLRWLFFVFVLTGFVSNAFLLQFGFYRIVFILQVILYLFGT
                     IGLIFQEKGHHIPLTGIPGYFVAMVYSSALAFWNFLQNKKINLWQPVR"
     gene            19566..20558
                     /locus_tag="PLW08_04460"
     CDS             19566..20558
                     /locus_tag="PLW08_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL51888.1"
                     /translation="MNILITGSNGFVGSNLMRELEEDGHTVIGIDISEHCEGKKHPQT
                     LRGDIRSLKDLNRVASVFLQKQQCPLELIIHCAAAKNDFGVSRQEYYSHNKYGTKTLL
                     NFACEQGIKKLIYISSVGVFGHPEGKANEDFPYNPDTDYGASKLAGELLCIKWQQQNP
                     EHELIVLRPAAIFGPDNFTNTYKLIDTLHRKPYLTIGEGNHIKTIVSIHTVIDMIRFA
                     MQKLAPGYEHYNCIDEPYLTLKELMELICSHPGFQMPRLKIPLKAAIGIGRLFDIPAK
                     LFSIDLPVNSERMRKLGTATYFTAEKAKKDGFVQKMSLKDSITEMCDWYLSINK"
     gene            20578..21606
                     /locus_tag="PLW08_04465"
     CDS             20578..21606
                     /locus_tag="PLW08_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPL51889.1"
                     /translation="MKTVKIGLIGCGRISKNHLDAVSQIPEAEFVAVCDLVEEKMQAV
                     AENYGIKNLYNNYQDMLENEKLDLVSICTPSGLHPQMGIDVAKHKINVLTEKPMATNI
                     ESADALIRACDQNKVKLFVVKQNRLNATMQLLKRAIDKGRFGRIYLAESNVFWQRPQS
                     YYDAEKWRGTWEFDGGAFMNQASHYVDALYWLLGNVDSVMAYTATMARRIEAEDTGCA
                     ILHFRNGIIATINVTMLTYPKNFEGSITIIGEKGTVKIGGVAVNKIEKWEFEDYDDDD
                     RIAQDANYQPPNVYGFGHNPYYRNVIDVLLGKDVPSTDGRDGRKSVEIIQAIYRSAKT
                     GKRVSLPL"
BASE COUNT         7176 a         3891 c         4206 g         6550 t
ORIGIN      
        1 cggaactctt tctcggaaaa ggagataatg actctcctgc tttcttatca ccgggttgaa
       61 acccgttgtt actatctgtc gttcctacgg aatttttact acaatatatg attgctgatt
      121 aggaaaataa gattaaccat tatttctact gcattatttg ggttaaactt aaacaatgca
      181 actggctcaa ttatgaacca tatttcgcaa ttggaatgcg gaattccggt ttggcaaaat
      241 tcaatagtgt gcaggacacg atttttcttg acacaataat atactttggc tataaagaaa
      301 aaaatcctta tagacaagga gaccaagatg aaaaagtatt tatttttagt attaatctta
      361 ttacctgtta ttttgttggg acaggcaggt tatggtgaaa taagcattcc aggtgaccca
      421 atgtgggaag aggaatcaga aactacagaa ggaacacatt ggggaatggg aggagctgtt
      481 ggagccgtta caattgataa tcagacctat tctcagattc gtttacagcc cgaacttcag
      541 gtttggaaat ttggattagg tttggatatt gatttaataa ttgatgcaga agggaaagtg
      601 cggaaagagg actgggatga atggcaggat tatgtaaaca aaattttatt tctcagattt
      661 gctcaacgca acgatccttt ctattttaag gttggcagta ttccggatta tacattaggt
      721 aatggtttga ttttcaatca ttattccaat atgacgctct atcctcaagt taaaaatgta
      781 ggtggttatg taggaataaa tactcatctt tcgggattag gttttgaagc atatacgcat
      841 aacctgcaca aaaatgagat cttagccggt agagtgcatt ttcaaccctt ggcagtaacc
      901 aatattcctc tgctggagaa attgcgagta ggagttaatg taggcataga tcgtgaccaa
      961 tatggaaagt atgaagataa agatggtgat aacattccgg atgtttacga taaatttccc
     1021 aaggataaaa acaggtattt ggataccgat aatgacggga ttgcagataa tgatgatata
     1081 gatattaacg gtaataatat tttagaccat ccttttgtta atccttatgt agcggaacac
     1141 tttccgggaa tagagaatct tggttatgtg ttggatatgg atgtaacacc cgatagtgca
     1201 gctgtctaca acgaaaaaga tgaggttctt gtttacagtt tggattacca gttaccttta
     1261 atagaatccg agcgtttcaa cctgattcat tatggagaaa tagccaaaat tgacggttac
     1321 ggaacaggta tgatttttcc cggattcagc agtaagttct ttatttttga tgcccgtttg
     1381 gaattccgca attttggcga tcaatttttg ccgggatatt ttgacaggca atatgatcaa
     1441 cagcgggctc aggtaaggca tataatttct gatggtaacg gaaatcaaat atggtattta
     1501 acgactaaag aagaaaccct gaaagatgtt gagtcctctc tcggctggtt tggctattta
     1561 agagccaatc tttttaatat tgtatatgct aaaattgctt atcaggatat gtatggcaag
     1621 caaaactttt tgggcaaatc cttgtgggca agcgttactg tatgtccttc tattgttcct
     1681 aaattgaagg aagcatcagt ttgttacgct caaaccaatg taaattatat caacttcaaa
     1741 tatcccagaa atgataatgc caatatttta gggcgcctgg tatattccat ttcggataat
     1801 gccaatttgg ttggaagtta cagagaatat tataccgata tcaataatga cggtaaaatc
     1861 aaaggtaaag atgaaataat tgaaaccttc acttttggag tggaattttg gttttaggta
     1921 ttaaccaata tctatgcctg aataatcctc aattttaagg aagggaaaca gatagcagaa
     1981 aattctttat tcacaagaac aatgatatag cgttctcttc tgttaactga tgttctaccc
     2041 aacatcaact tatccttaag gatgggtgct ttccaaggaa cagaaaagac aatatttaat
     2101 tgataaaata ggaatttagc gaggggctta cacctgcgga agaactgcaa gttcgtcatt
     2161 cacttgtaat tcaactattt cctgattatt aatttggggt aataaagtgg attggaaagt
     2221 atagttgaag aggatttcaa gcttccgggt tctaaacctt ttcaaccgtt tcaaccttta
     2281 taagcgggat ttgataaata tgttgctata aatatatcag ggaattgacg caataaagag
     2341 gtctgcagaa ccggatagag ttacaggtct ttccgcaaaa gagataaaca ttgccttacc
     2401 tctttccaac gagaaaccgt cctcggtagt aaaagaaccc ttaaaacaaa agaccagcaa
     2461 gggtttatgc aaattatcca aaatgtaggt tccttccaga ataagccgtg aaagatagaa
     2521 ttcttctgcc ggagtagggt atgaaaccag ttcattaatt ttcatatgcg ggtgaattaa
     2581 ttggggcagg tatgtttcaa aacgggtgat ggcaaaaagt atttcccggt ttatatattt
     2641 gggagtaagt cctccgcgga taacattatc ggaattagcc ataatttcaa ttccggctcc
     2701 ctggatatag gaatgcagaa ttccggcatc taaaaaaatt gcctgacctg gttgcaaggt
     2761 gaaagtattg agtaataagg ggctaactat gccactatca aggggataga attcatttaa
     2821 gtgcttacaa atttggcagg tgaatttcag tttagggctc aatttcggtt ggattatttc
     2881 aaggcaatgc ataacttctt ccagttgtga acttttgctt tccagaattt gactgaataa
     2941 atccctcaag gtatttttag aaggattctt gttgaaagcg gagaatttct gccaaatctc
     3001 tattatgcct aaaatatgaa aattgcggat aatttcatca aaaggtcgga atccgcacat
     3061 tgcaataaaa ggagtaagag cacatatcaa ttcgggttta tgattggggt ctttgaaact
     3121 gcgtttggga tcgtttaacg gaattccttc ctgctcttct ttacgaaagc cattttgggc
     3181 agtttgctta tcaggatgaa cttgaagaga aagaggttct gcggcagcta aaattttcaa
     3241 gagaaaaggc aaggcaatat tttgttccgt tcccagcata ttttccggca tatcctgcaa
     3301 caatttatcc attgggattt ctcctttttc ggttattacc agggaaggtg ctttgggatg
     3361 tgctccaaac catatttcgg ctaagggttt tcctatttcc tgttgttcac caatcaattc
     3421 ctggaggtat ttagtatcac cccaggaata attttggagg gacggtttaa ttaaataggg
     3481 cataaataaa ctccttaaaa ctgaaaataa ataaaaggtt gaatttcagc agagcgaaat
     3541 tggaataaaa tccatatcac tattgctaaa atcaacgact gtaaaattac cggtgcttta
     3601 ctaagcgttt tttgtaaagc attattataa ctatccggca tccagtgaaa cacaaaaccg
     3661 gttataataa gaatagcaca tattttatat tgagttagcc actgccagaa aatgcttccg
     3721 ttgaaagcag taaaaatgcg gttcaacatc attcctgcgc taaaaaaatc gggtgaacgg
     3781 aaaaatatcc aaccgaaaca gacaaaatgg aaagtaacga ttacgccgat aactttcatc
     3841 actttggttt gcattatctt taagcgtaac agcattttat ccaaaaccaa agccaaaccg
     3901 tgaataccac cccaaacaac aaaattccag gatgctccat gccacaaacc acccaagagc
     3961 atagttatca tcaggttgag atattgtctg gctttacctt tacggtttcc tcccaaagga
     4021 atatacagat aatccctcaa ccaagtggaa agagaaatat gccaacgccg ccaaaaatcc
     4081 gttacagaac ttgccctgta tggagttaaa aagtttaccg gtaaagaaaa acccaatagt
     4141 gctgccaatc cgatggcaat atcggaataa ccgctaaaat cacaataaat ctgtaaagca
     4201 taagcataaa cgcctaaaag attttccaag ccggagaaaa gcgctggatt ttcaaaaatg
     4261 cggtctacaa agttgaccga taaataatcc gctataactc cttttttaat taaacccgaa
     4321 aagatcaaaa tcagggcttt ggctatggta tctttatcta aacttaactt tttatgaatt
     4381 tgagggataa aataactggc acgaacaata ggtcctgcta ccagctgcgg gaaaaaagaa
     4441 acgaaaaagg tgaaatccac aaaacttttc agcggtttta gtttgccgaa atagatgtcc
     4501 agagtataac tccaggtttg aaaagtaaaa aaggaaatgc ctacaggtaa aaagatatca
     4561 taaagaggaa gttgcccgcc aaaaacttga ttgatagtag taattataaa attggtgtat
     4621 ttgaagtaac ccagcgaacc taaattccag aacacactaa gccacaaata aaaacgcttg
     4681 gtagctttac tctgtgcatt gtaaagaaaa gcacccaaac caaaattgat gagggaagta
     4741 ataagcaata aaattatgaa actaccgctg gttttgtaat agaaatataa agaagccaac
     4801 atcagatacc aggtgcggac tttaactttg ttcactacca gaggatagaa aagcaaaacc
     4861 acaataaaga aaaacagaaa gaaagtgctg tggaaaagca atggcttttg agggtcgtaa
     4921 actaaactat tcaggatatt ttggattaag ggaggcatag taattttctc ttatcccgat
     4981 tgttcgctga aacttttgta acttttaata aatgcctgat agaagagata gccctgcaac
     5041 atatagccct tgggggtaaa atgcaaaagg tctttagcag acatttgttg gttgcgccat
     5101 ttttggatac tgttattgcc acccatcact tcgttcaaat tccaccaggc acattgattt
     5161 tctttggcat agtcaatgat aattttatcc accttgtcca gatcgctatt gtgctttccc
     5221 cgcttgttag aatccggagg aagagtgaaa agaactggtg tttcgggatt gaactgcctt
     5281 agtttataca taaaggtatt caagttttta gtaaagacct ctccgcgaaa agtgccctgc
     5341 gcatcgtttg ttcctaaaga aataacgatt aaatcggggt ttaaaagtcc tatctgctca
     5401 aacaatatat gttgctccag cagattgtaa aaaccggctc cgttaattcc acaagagtga
     5461 tatagcaatc ctggttgttc tttttccaag caaattccgt ataaagtaga ggattcaccg
     5521 gttaatgtaa gttttacttt ggatacttct gtttcccagt aaactctgct gtaatagtag
     5581 ttgcctattt tttcggcatt cttttcgggt tcggcggaag caaactccag ttttgtttcc
     5641 attccgggta gcgaggttaa cagagataat ttattaaaac tgttatctcc tctgaaaaaa
     5701 ttgttggtag ctatttccag gcataaaccg tctgccattt ttaaagcata accgcttaat
     5761 ccgcgttcag aatttaaacg ggtgaaagag ccgctggctg atattctata gtcatccggt
     5821 tgatttgttc ctgccagtcg taaaggaaaa actaatcccc tgcctgcatt gccgaaatat
     5881 tgttggagtg aagttctggc agttccagaa aaatatcctg cttgaatatg ggaatcgcct
     5941 atttgataga tagtaaccgt ttgtcttaag ccactacgca gttccagtaa tttttcataa
     6001 aatggtttta gagctttttt atcgcccaga aactgatttt tatccaggtt tagaaattta
     6061 tactgtttta ggtattcatt actttccaga agcaaataat agctttgaat aaattcttcc
     6121 agagtatcca tatcggaagg atattcctct tcgctgaaaa gttcattgat atcttcagtc
     6181 gtaatatttt cttcggcata acttttgctt accggaaaga tacaaaccag tgccagtata
     6241 caaaaaggta ataaacggcg gatatgcact taatttcctt tcagataagc aagaagcatt
     6301 tcagcaatat gatcagctcc tgcccgtgta aaatgagtgt aatcctttcc tgccaaagga
     6361 ggttgatgat ttacatattt tggcatagaa ttcagcccac ccattgcaga aaataaattc
     6421 cagaaaccac aacctgtttc tttggcaatt ttggcttgag ttgaaaccag ataaggtata
     6481 tcaggagaag ttacatactc cgttccctgc ttaatacatc tatcgtgtgc gctgataagt
     6541 aaaatcggaa ctccgggaag cgctttttga agatgctcaa tgcttttttt catccctttt
     6601 gcgtaatagc tgtaatccct aatttggtaa ttggaaacat tcactccata ctgcaggatg
     6661 ataaggtcgt aatgcaagtg tttctgaaaa ccggataaaa catcagaact tatcctctga
     6721 aaatacattc ccgaataacc gcgaatggga tagttatcta cataagctcc ttgcggttcg
     6781 tcaaagctaa ctccatataa atgaatagga tcataagcat taaattccag ccggattttt
     6841 ttacaggcag taacaggact gaggtctaaa acctgaaccc cgggctgatt ttgtaaatga
     6901 cgcaagataa agggagctcc attataggag acctttacat aactgctatc cgaactgtga
     6961 ctgtaaaaaa gacgaatacg attgaaagtt gaagctcctc ctgctacctg agatgcacta
     7021 tattccaccc aagggtcata atcaacataa aaacgggcac tttctttttg cggttccggg
     7081 cttgctacct gtgcggaatc caaaacagct aaactatcaa gctgtaaggg ctcaataacc
     7141 ttttccgcta cataataatt acgcggaata aaagtgtaac cggtaattcc caaaggaata
     7201 tcatatttgt gaggagtcat aaaggaaatg ctttcccagt tgcgggaaaa actgtgcttg
     7261 atagtttgcc tgaaacctgc tacaattgaa gttataccta ccattcctac accggaacct
     7321 tgatgcattg attgaagttc catccgcagt tttccggtta ttaaatcccc ttcaataata
     7381 gaatctccat aataggcaat ccgtaaattc tccgaaggat tattgagttt ggtaataaat
     7441 tgcttaagcg gtaataaatc attggaaata gcaaccgctt tttgcacaaa ggaggtatct
     7501 gctgagacaa ctgctttctc gcgtttcaga gattcaaacc aagaaagctt ttttaaaggg
     7561 aaaaaatggt tatccacagc aggaataacc ctggctaaaa tttccactaa aatgaccgct
     7621 aaaaccacta ttataagtgg tctgataaaa tttttaggca taaccacctt ttaagttata
     7681 ataatgtcac tcacagataa cacagattta taattttgct ttcctttaca ccgagaacac
     7741 cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttcc aatttttata
     7861 tagtaagtta ccgtcaagaa ctatttggtt ttatgatatt ttaccttaat atatctataa
     7921 cgaataaaag gagtgcttta tgaaaaagac aaaattcggg ttgttattag gcataggaat
     7981 ttgtgctata ataattgtat cctgttctgc tcctgaaact tattcttcaa aaccggtttc
     8041 tcaatctact gaacagaaaa ctccggaaat agaaaaggtc tctaaggtag taaattacca
     8101 ccaggataag ttgcaggcaa tttataaaaa acatacttac caaaatgcaa tgcaaggcga
     8161 tttggaattt acactttata ttactaatga ggggaaagtt caggaagtag aagtgaaagc
     8221 actttccgga aagtttttct ccaattttat tgatgaactg aagaaggaaa tttttacctg
     8281 ggcatttcct aagcaggata aaattatcta tagttttgtg gtgagtttcc gaaaagggtg
     8341 agtttttata aagagaaaaa gagagttctt ctggaataat tacttagagc gaggaaacat
     8401 aaaaagcccc attcttcatt cttgtccttt cttcttgaca tcaatgtgta tgtaaatctt
     8461 taggttacaa cttataaaaa agaggatgaa agcgtggaat acactgatta taaaaagacc
     8521 gccaatgata tgatagaaca aatttctgaa attcggaggt tactttgatc ccgagcccaa
     8581 aaagcatttg ataatggaac tggagcaaaa gatgaatgct cccgattttt gggaagatca
     8641 aacacaagcc aaaaaaataa gcaaacagct tgctcaatta agagatgaac tggagcatct
     8701 gaaaaaactg gataccgtta aaagtgaact ggagatatat cttaccttat tagatgaaga
     8761 tttcaatgcc gaattactgg cagaagcggt ttctgaactt ccccgcatac aaagttttat
     8821 tgaaaaagct gaaattgagt gctatctgaa cgataaatat gaccataata atgcactgct
     8881 gacaattcat tctggtgcag gcggaacaga atctcaggac tgggctgaaa tgcttttacg
     8941 gatgtatgct cattgggcag aaaaaaatga ctatagcttt gatattattg attctctacc
     9001 aggagaagaa gcaggactta aaagtgtaag cgttgaaatc aaaggtgatt atgcttacgg
     9061 gatgttaaaa agtgaaattg gtgttcaccg tttagtgaga attagccctt tcaacgctca
     9121 aggtaaaaga cagacctctt ttgcctcggt ttttgtgtat ccggaatttg atgaagacct
     9181 ggaaatagag attaacccca aggacttgaa aatagatact ttccgtgcaa gtggagcagg
     9241 aggacaatat gttaatacca ctgattctgc agttaggata acacatttac ctacaaatat
     9301 aaccgttacc tgccaaaatg aacgcagtca gatgcagaat cgggaaaaag caatgtccat
     9361 tttgaaaagc cgtttatatc aatactatga ggaacaaagg gaaaaagaga aaaagaatct
     9421 ggaaaatagc aaaacggaaa ttggctgggg caatcaaatt cgcagttatg ttttccatcc
     9481 ctatcagatg attaaagacc atagaacaaa ttatgaaaca ggcaatgttg ataaggtaat
     9541 gaatggcgat ttgaatgatt ttatttatgc ctggctgaaa tacaatgcac atcagaggat
     9601 aaatggctaa gcaggattat aaagaattgc tcaatgaact ggataaaaac aagttacctg
     9661 ttcatatagg tttcattatg gatggaaatg gaagatgggc aaaaaaaaga aatagacccc
     9721 atctttacgg ccacagagca ggtgtgaaag cattaaggca agttgtagaa ttgggagttg
     9781 agctaaaact tcaatattta accttctatg ctttttctac agagaactgg agccgtccgg
     9841 aaagcgaagt aaaaggtctg ttaagattgc ttaaagaaca gctaaaaaag caaattccgg
     9901 aactgatgga acaaaatatc tatgctcaat ttattggttc tactgttggc ttggaagaaa
     9961 attactggaa agaagtaagt tctctggttg ctcaaacaca taataacaca ggaatgatag
    10021 ttaattttgc cttcaactat ggaggtcgcc tggaaattat tgagggcttc aaaaagttta
    10081 tgacgatgca tcctgaggaa tggcataaac taacccctga ggaatttgga aattaccttt
    10141 ggacagctgg tcagcccgat ccggatttga taattagaac cagcggagaa aaacgtcttt
    10201 ccaatttttt gctttggcaa tccgcttatg ccgaactcta tattacagat acattgtggc
    10261 ctgattttga caaagtggaa ctgataaagg cgcttttaga ttatacttcc cgggaacgca
    10321 ggtttggggg caggaatgag tgaactggca aaaagagtgc tggttgcagt tattttaatt
    10381 cccatagttc ttattgttct gtattatagg ggtttgcctt tagttataac tttattgatt
    10441 attacttttt tgagctcctg ggaatacgct caaatgctaa ataaggcagg cataaaaata
    10501 cactatgttt ggcttgcgtt caattctctt ttatatttgg cttttgttta tatgaagggt
    10561 aaggatttat ctcttttatg gttggtcttg tttttagcaa taggggaagc aatattaacc
    10621 tgggaaaagg aattatctgt tccccgtatt tttgctgttc ttttcggttt cgtttatact
    10681 gctttatttc ctgctttaat tgctcgtttg ggtttatatt atgcggaaca taatttttta
    10741 cttgccttaa ttctattgat ttggattgtg gattccgttg cttattttgc tggaataaaa
    10801 ataggaaaac atagaaatat aacagctatc agcccgcaaa aatccctgga gggttttata
    10861 gcgggagttc ttgcgccttg gctgattttg attatattat atatttgcaa ggttgagata
    10921 ctgccatttt cttatctggc tctgctggca gttgcaagtg gaattttcgg tcagataggg
    10981 gacttggcag agtcaatgtt aaaacgattt tgcaaggtaa agaacagttc taatcttctt
    11041 cccgggcacg ggggaatttt agacaggtgt gacagttgtt tatttgcagg ttcgttttta
    11101 tattgtgctt tggaaatttt aactaaagtg aggtaataat aatgaaaaaa gcacttactc
    11161 taatcgttat gttagctgcc acagcagcta tgtttgctca ggtctatgcc gttgaccttt
    11221 tcttctcgga atatgtagaa ggaacaagca acaataaggc attggaaatt tttaacggaa
    11281 ccggagagcc agttgattta tccaattaca ccgtaaaact tgcctccaat ggaggaacct
    11341 ggagctctac caacattctt aatatgagtg gagtgttgga tgatggtgat gtttatgtaa
    11401 ttgccaacag ccaggcagaa gcatcaattc tggctattgc caatgtaacc tccactgtta
    11461 catactataa tggtaacgat gtagtgggat tatttaatgg aaatactctt attgatatta
    11521 tcggtgttta tcaacaagac ccagaaactg ccaattggcc tgttgccgga actccttctg
    11581 ctactgcgga acatacttta attcggaaac caactgttat tcagggaaat actgattttc
    11641 ttgctggtgc cggaactaat gctgacgatt cggaatggat agtttatccg cagaattatt
    11701 ttgccgattt gggtcagcat acttttaatc ccggagctcc cgaacaagca gcaactccta
    11761 cttttaatcc tcctgcaggg gtctatgcaa atcctatcag tgtaacaatt tcttctacaa
    11821 cgcctggagc tacaattcgt tataccacag atggcacaga accgaatgaa acatccactc
    11881 tttattctgc tcctatagct ttggcaacta ctacaactct gaaagccaag gcctatgcta
    11941 ccggttttag tccttcttac acagcaactg ctaattatat cttccctgtg caagtttcca
    12001 atattgcagc tttacgtgct tctgcagccg atggaacaac tatttatcag cttgccaatg
    12061 aagtaatact tacgatgcaa cagaactggc gtcaccaaaa gttcattcag gatgcaacag
    12121 ctgctatttt gattgatgat tataataata ttatgaacac tccttataat atcggagatg
    12181 gaattacagg cattacagga actctaaatc gttacactac cggtatgcta caattttggc
    12241 ctgtaataaa tcccggtcct gccacctcta caggtaatgt aattattcct caattggtaa
    12301 ctatagcaga gataactaac aatgcagaac tctatcaatc acgtttaatg cgtctggata
    12361 atgttcacta tgctagcccc agcggaaatt atgccgctca aaccagttat gatttattgg
    12421 atgctacggg cactattgta atgcgaactc aattctctga tgcggattat attggaactc
    12481 ctatgcatca gggtaatttt aatgtgtatg ttatagttac tcaatataat gctacaacac
    12541 aggttactcc cagaatgcta agtgacttca atcccgttgc caatgatgat aatgtgttaa
    12601 cacctatgca aattgaactg ataggcaact atcctaatcc tttcaatccc gaaacaacta
    12661 ttcgtttcaa aatggaaaaa gcagctcctg cggaagtgat tatatacaac gataaaggtc
    12721 agatagttaa aacatttagc actattgcta cgcagggaat aaacagtgtt gtttgggatg
    12781 gaaaagataa taatggacat cctgttgcca gtggagttta tctgttccgt ctgaaatcag
    12841 gaagctacag cagcactaaa aaaatggttt tgatgaaata gccggaggat gctgttcctc
    12901 ctcaagaaaa aagggagatt cagcgagctc cgactacacc aaaacgactt ttgactacac
    12961 agtaaacagc atactgacaa ccaactattg gaaaagtaag aaagcaaaga tgtcagtatg
    13021 ctgttttaca atttagaagc aatgccaata tccaaacgaa aacttctgtt gataacggat
    13081 ttatatccca ataaattcaa tcctgtaaat ggtgtgtttg ttcagcagca ggcaattgaa
    13141 ctttccaggt attatcaggt tcaagttgtt gctgcttgtg ataaaaatcc ttatcttgta
    13201 tatgaacatc atcaggatgg ctttccagta aaactaatct gctatcccta ttggcaaaag
    13261 tattttttaa cttccctgat aacatataga atattggctt taccagccat aaaaagatgc
    13321 tataaaagat ttcaaccgga tataattcat attcatgatt accgtcatat tccggaatta
    13381 ttttggttga agccctggtt aaataaactt tctgttccca agtatctcac tttacataat
    13441 atacggactc atccggaacg gctgaaaaat aacccccgga ttggttttta ccgttgggct
    13501 ttaaagaaaa cactaactgg ttggaagcat atttttaccg taaatagcaa aatagctaaa
    13561 tgggtaaaac aatataaccc tgcttccaaa gtaacgataa taggcaatgc tatttcagag
    13621 cttgctgaag ttgatccgca attaatagag ccatataaaa atattttgtt agctgattat
    13681 tttcatctta tcagcatagg taacctgttt accgagaagg gcttttcata tttgattgaa
    13741 gcaatatcct tattaaaaaa agaaggactt caagttcagc tcattatagc aggggatgga
    13801 gatagaagaa aggaactgga aaatcaggtt aggaacaata acttgaatga ggaagtgata
    13861 ttccttggca gaattgagaa taaactgtta agaaatatcc tgcctttgtt tgatttattt
    13921 gttctggcaa gctatagtga aacctttggc attgtatttc tggaggcaat gtttgccggc
    13981 ttgcctgtaa ttggcattaa agaagagggt atctacaatt tggcagaaga cggtaaacaa
    14041 gcattatttg cagagcccaa aaacagtaaa gacctggctg ctaaaattaa attactgatg
    14101 atgaatccgc ttttaagaga ggagatagct aaagcaggac aaagattggt gcaagagaaa
    14161 tatatgctaa aggaacttat taaaagaata atagcggttt atgaacaaaa ataagatact
    14221 gatgattatc aacgaatttc ctcccacagg tcaaagcgga gtccaaagac ctttgaaatt
    14281 cgttaaatat gccgtaaaag ccggatggga ggtgcatatt attgctccaa aaaagccggt
    14341 aagaaaagtg gtagattatt cccttctgca ggaaataccc aaagaggcac atatttacag
    14401 agttgctggc ttaggcatta agaccccaga tgaaagcaaa atggtaaatg cccattttgc
    14461 ggaaacagct ccaccttcca agatagaaag agcattttgg agattggcaa agctggtaaa
    14521 tgattttctt ttcccttatg ataaacaaat cggctggatg ccttttgctt attatactgc
    14581 ttgtaagatt attaaaaagc aacagattag aaatatttac atcactgctt ttccctattc
    14641 tgcttttctt gttggtttgg ctcttaagaa gaagtataag gacaaaattt tctgggtagc
    14701 ggattataga gattcctggc agtttggcat aatgatagaa aaattagtat ttccctttcg
    14761 cttgaaaact atccgcaaaa cggatgataa agttctggca acttgtgatg cagctgtttt
    14821 tgtaactccg gaaacccaat tggaatatat aaacaaacat agctggttga aagaaaagtc
    14881 ctattatatt cccaacggct atgatgaaga tgattttaca ggtattaccc cgaaaaagtt
    14941 tgataagctg actcttgttt tgatgggcaa gctaactaaa gtttatggtt ctcccttaaa
    15001 tttactgaag gcactggagg actgctttcc gaataattat caggtaatcc atataggcaa
    15061 tatagataaa gctattttgc acaacataaa aacctccggt tacacgagct ataattatct
    15121 tggctatcaa aaacatagcg acgcaattgc ctattccctg ggagcagaca tcaatttgat
    15181 tatcctaaat gaaaattcca ctgctaaata ttggtatcca ggcaaacttt ttgaactttt
    15241 acgctgtggg aaacctattc tggctttagg accacaggaa agcaatctgg agaaaatatt
    15301 aagcgctacc aaacgaggca gatacgcata tattaatgat aaagaacaaa taaaagccca
    15361 aattgaatat atcctaaaaa atcccgatca gtttgatacc tctcccgaaa cgattaaaca
    15421 atattcccga gaagaattat gcaaacagtt attggcaata tatgaaaaag gtcaatccag
    15481 taattcctga acgcgtttaa ataccatatc cggagtaagc ttaagcagac aatccagagt
    15541 acagtttggt ttctttttct tatctatgca gggagcacag tcaacaggat agcgaattat
    15601 gttactatta tttgaaaaag gagctgtctg taactcgttg gcaggaccaa aaatagttaa
    15661 cccctttatt ccaaagcagg aagcgatatg tcctaaaccg gaatcggtat taataaagaa
    15721 atgtaatcgg gcaataattg cagctacatt tgccagtgga gcattgagca gaagaaggtt
    15781 cttagttctt gcctgaatag cttttcctac ttcaatttca tcaggacctg caataatcaa
    15841 aataggataa tcatattgcc ttttaagttt ttctattaaa gcaataaaat attccttatt
    15901 ccatcttctg cgtttatttt tagccataca accaggatgt atgccaataa tcttttttcc
    15961 tgttagattg ttttcagcca ggaaattttc tgcccattga tgatttgctt ccgttaaatg
    16021 aaaataggga tataaattta gttcattttg acgaacaatt gcttcttccc aggaaggcag
    16081 atttaggaaa ctttgaaaca gagtatagtt acttcgggtt ctggaaagaa gaggattaaa
    16141 tttaacatag acggataaac ccggatagcg aaacttttta tattctccaa ccttgatttc
    16201 tgcctttaag agtaacataa aaagccaggt tttccagggg cgaactccgg aagaaaaaag
    16261 gacagcttga tacttctctt tccgtagttt gaaaagagta ggaagcagta aatttgcttt
    16321 ccagccacta aaatgaattt tactaataaa aggagcattt gttacaggta ttgcagcgga
    16381 cttcgggaaa aaaagaattt ccagttccag caaaggatat aaggtcttta ctgcttgtaa
    16441 aacaggagtt aataaaatca gatcaccaat tccccaagta tgaataagta aaacttttcc
    16501 ctgtgtaact tttttcattg taaagacctt aagccgtttt atgcaaaaat ttttcccaaa
    16561 gacctttcaa atcagccatt gtttcctgcc ataaatcaat aatatctttt ctcgttggta
    16621 agaggtattt tagcattttg ccctggctgt ccttttgttc aaaataaaca attaaaataa
    16681 accagagaaa ataggaaaaa ctactgctga gtgcagctcc gttgattccc ataacgggaa
    16741 tcagccaaag atttaagcca atattaatta ctaaagccac agcgggaagg ataatactga
    16801 ttataggaaa gcccttacta ttcaagtaat tattaaacag cgaaccaaaa cttaaaccat
    16861 aaacagctgg cattaaccaa aaataaacat aatacacagg cacaaattga actccaaaca
    16921 ttatgcgcat tatgaatttg cccagaagac caaaacctaa atttgccaaa gttataaaga
    16981 aaaagaacaa cagaagggtc ttcttcagaa gcagccattt actaatatca tcatctatat
    17041 cggaaagctt tacaaacaat aaacccccta caaggtttga agcaatctga ataaaatcta
    17101 caatatgtgc cgccatagaa taaattccta cttccttgaa atccaaaaaa tgcttgatta
    17161 ttacaatatc ggctctaatt agtaacataa tgaacagaga agaaagaaaa acacgaaaac
    17221 cataactgta aaacagctta actaacttga tatttaactg cttataccaa ttagtccatt
    17281 tggttcgcaa tactaaataa ataatactgc taattaaacc tgcaacggta aaaaccatga
    17341 ccgcattaat tctatctact tctaacagag caaaataacc aataagcaaa agaacaaaaa
    17401 agatggcgga acttaaaaaa tgcgccaaag aattttccag aattctatcc attcccatat
    17461 atattgcctg caattgcata aatgccttat taaaagaaat aaacaatatt aaaaggatca
    17521 tttttgccgg tgtaagagga taaccaatca atttactcca aaaatccaga aaaacaattc
    17581 ccagacagct gaaaatgagc aattccaaaa acatcgtaag aatagtccag ccaaacatcg
    17641 ttttcacttc atcaggatat ttacgcacta aataaggata acttctgaaa attccccaat
    17701 ccaaaatcat ccagataaaa cctcccaaag taattaaata gctgtattca ccttttaaag
    17761 ttatacctaa atacctggca cttatccagc tgattaaaaa tgaaagaatt aggattacta
    17821 tcctgaaact gccactggta acgatatttt tcttcaggtt tatctgggac atttttctct
    17881 gtttttgctg tgtaaatttt ggtttttact ataattcata atgccataat gcttatatcg
    17941 tcctttttgt caaatgtaaa aaaaatcttt gctcctcgct cttagccctc agccaaaagc
    18001 acgtcagggc gagacaatga tagcgatggt ggcgtaagcc cctcgggaaa tggaaaatga
    18061 agaatgaatt aagtaaggaa ttgtcatcgt ttatataatt ttaaccttta cctcaaatta
    18121 ttttagcgat gaggactggt gacgacttgc ttttattaca aagagaaaag agaaaaagag
    18181 atttaatgaa tagcgaaaaa tgaagataac tcggaaacta taaaaataac ctcggtgttc
    18241 tatgtatttt cggcgtaaac caaaattaaa atcataactc tatgtttttt tattgtgagg
    18301 tgtaagtgga cttaatttcc ttgacagtta acagcttgat tttattggga actttcaagg
    18361 taaatgcaat gttaacttta tataagacac acagaaaagg acaataaatg gtaatcgcga
    18421 agatagtatt ctggatttcc tggctacttt tagcttacca ctttgttggt tatggattaa
    18481 tcttatttat cataacttcc ctgtttaaaa aagcaaagat ttcaattccc gcaccggatg
    18541 tctttccttc catcactgta atatgtcctg catacaacga ggaaaaggtt atagaagaaa
    18601 aaatccgttc ctttttacag ctgaattatc ctaaagacag aataaaaatg attattattt
    18661 ctgatgattc caccgataaa accaatgaaa tagtgcaaaa atataccgat caaaacattt
    18721 ctctggtaat tcaaaagcca agagcaggca aacaaaatgc tcataatctt gttttacctt
    18781 tactggatac cgattatgtt ttatctaccg atgccaacag tatttttact cccgattgtg
    18841 ttcaactttt agtagctaaa atgctttctg ataaaaggat aggtctcgtt tctggagaag
    18901 tgaaaatggt taaaagaggc agtaaacaat cgggggaagc tcactattgg aaatacgagg
    18961 catttttaaa attgatggat tcacggctaa aaactttaat cggagctaat ggtcctctct
    19021 atttgattaa aagagaactt ttttcggaaa ttcccccaaa cagtcctgat gattttgaac
    19081 gggttttact tactttaaaa cgaagatata tagctgctta tgaaccacaa gccatcattt
    19141 atgaagaagt taccgaaaaa gcgacggaag aaatatcccg aaaagtgagg attattaccc
    19201 aggaatggtg tgttttgaaa cgcaatattg agttattaaa tccgtttcgt tatcccgctg
    19261 taagttttat tttattttcg cacaaagtgt tacgctggtt attctttgtt tttgtattga
    19321 caggatttgt cagtaacgct tttttgttgc agtttggttt ttacaggatt gtttttattt
    19381 tgcaggtgat tttatatttg tttggaacta taggacttat attccaagaa aaaggtcacc
    19441 atattccttt aaccggaatt ccgggatatt ttgtagcgat ggtctattcc tcagctcttg
    19501 ctttttggaa cttcctgcaa aataagaaga ttaatttatg gcagccggtg cgctaaggag
    19561 aaaaaatgaa tattctaatt accggtagta acggttttgt cggttccaat ttaatgcggg
    19621 aactggaaga agacgggcat actgtaatcg gtattgatat cagtgaacat tgcgaaggga
    19681 aaaagcatcc ccaaacgctg agaggagata tcaggtcgct aaaggattta aacagggttg
    19741 cctctgtttt tttacaaaaa cagcaatgtc ctctggaatt gattattcat tgcgcagcag
    19801 cgaagaatga ctttggtgtt tcccgtcagg aatattatag tcataataaa tacggcacca
    19861 aaacattact gaattttgcc tgtgagcaag gcatcaagaa gttaatttat atcagttccg
    19921 ttggcgtttt tggtcatccg gaaggtaaag caaatgagga ttttccttat aatccggata
    19981 ctgattatgg cgcttcaaaa ctggcaggag agttgctttg tattaagtgg cagcagcaaa
    20041 atccggaaca cgaacttatt gttttgcgtc cggcagctat ttttggtccc gataatttta
    20101 ccaatactta taaacttata gatactttac atagaaaacc atatttaact atcggcgaag
    20161 gaaatcacat taaaaccatc gtttccatcc atactgtgat tgatatgatt cgttttgcaa
    20221 tgcagaaatt agcccctgga tacgaacatt ataactgcat tgatgagcca tatttaaccc
    20281 tgaaggaatt aatggagctt atttgttcgc atccaggttt ccaaatgccc cgccttaaaa
    20341 tccccttgaa agcagcaatt ggcattggaa gattgtttga tattccagcg aaactttttt
    20401 ctatagacct tccagtaaat agtgagagga tgcgtaaatt aggaaccgct acctatttta
    20461 ctgccgaaaa agctaaaaaa gatggttttg tacaaaagat gtccctaaaa gatagcatca
    20521 ccgagatgtg cgattggtat ctttctataa acaaataact taacaggagt attttggatg
    20581 aaaacagtga aaatcggctt aattggctgt ggccgcattt ctaaaaatca ccttgatgcc
    20641 gtttctcaaa ttccggaagc ggagtttgtt gccgtttgtg accttgtgga agaaaaaatg
    20701 caggcggtag cagaaaatta tgggattaaa aatctttata acaactatca ggatatgctg
    20761 gaaaacgaaa agttagacct cgtttcaatt tgcacaccaa gtggcttgca tcctcaaatg
    20821 ggaattgatg tagcaaaaca taaaatcaat gtgttaaccg aaaaaccaat ggctacaaac
    20881 attgagtcag ccgatgcttt aatccgggct tgcgaccaaa ataaggtgaa actttttgta
    20941 gttaaacaaa atcgcctcaa tgccactatg caactcctta aaagagctat agataaagga
    21001 cgctttggca gaatctattt agcggagtcc aatgtttttt ggcaaagacc tcagtcctat
    21061 tacgatgccg aaaaatggcg tggaacctgg gaatttgatg gcggtgcctt tatgaatcag
    21121 gcaagtcatt atgtggatgc actttattgg cttttaggaa atgtggatag tgttatggct
    21181 tacacggcaa ctatggctcg ccgaattgaa gcggaagata cgggttgtgc tatcttgcat
    21241 tttcgcaatg gtataatagc aactatcaat gttacgatgc taacctatcc caaaaatttt
    21301 gagggctcta tcactatcat cggagaaaaa ggaacggtta aaatcggtgg tgtggctgta
    21361 aataaaatag aaaaatggga atttgaggat tacgatgatg atgatcgcat tgctcaggac
    21421 gccaattatc agccgccaaa tgtgtatggc tttggtcata atccctacta tcgcaatgtg
    21481 atagatgtcc ttttaggtaa agatgtccct tccaccgatg gaagagacgg cagaaaatca
    21541 gtggaaatta ttcaggcaat ttaccgttct gccaaaaccg gaaagcgcgt ttctctacct
    21601 ttataaagta aggatttttt tccccttagg gcagcaaaat catttagcat acaatttcat
    21661 accttgagct gccgaatttc aattcggaaa aggcaatgcc gaaaatgttt ttggaaaact
    21721 ataggatatc atttctattt tcatagttcc tttgtcattc ctgcgaaaac taaaatccag
    21781 atatttttaa tctatttaac tgaattttta atattgaaca tattatctgt gccaaattgg
    21841 aatttaccac ccttttttcc ctcaactatg tttttctgct tcatcttttt ttgcctgcat
    21901 aaccatctaa taatccagta aca
//
LOCUS       DAOOZV010000031        21810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2542_length_21810_cov_57.925672, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000031 DAOOZV010000000
VERSION     DAOOZV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_21810_cov_57.925672"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1018)
                     /locus_tag="PLW08_04470"
     CDS             complement(32..1018)
                     /locus_tag="PLW08_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPL51890.1"
                     /translation="MPKKIIYTVLLFLLIGSCLYAGKKKPEWISNPYSQYPSKQYLCG
                     VGMAKELNIAQNEALKNIAGFFETRVIAVTKSMESEKSSSSNYQTSSETSSSVKAIAE
                     GTLKYTQIKETWQDPKTKTFYALAVMDKIAVQTIYRNEIDDCEEFIENCLAPNDDPLI
                     RFAKILSAIDKLQQTQNAYTYYNALASDSGILLKPFHSLQELLELRNYAAREIPLQIR
                     VFGDSSGLLDASLKNVINQIGLLENDNPEYQIVATMNPEPHKSLGQQFFQPLRLNISF
                     NYGEKNLFSFVDKTEQGGSTIEEATSRGIRVLAQNIENKFRREFNQFLLNLH"
     gene            complement(1117..1836)
                     /locus_tag="PLW08_04475"
     CDS             complement(1117..1836)
                     /locus_tag="PLW08_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51891.1"
                     /translation="MRTIQRLIVLICIFLLLVSCVGLEAKKKKADAEVKLFLDDSQLD
                     NPEYYNKLPLLSLEAFASALLMPKTSNFKVINYNYKLAKAESKIAEEMVAVSLGMRQE
                     YVKIEAEGKSLMSANLEGSDTEISETLKAKLEDLKTDKAQLSESQKVYFLSGFIYLAS
                     SIVREKTILESSKKFVNEAKNAKGIDAVMYVKDIPYATNVVIGLPVLLSNQMRTISEF
                     ITIAKTQNIEIPKDVTDSLFQ"
     gene            complement(1850..2968)
                     /locus_tag="PLW08_04480"
     CDS             complement(1850..2968)
                     /locus_tag="PLW08_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51892.1"
                     /translation="MRKLLTIILLGITLLFSFAYLDAAVKVKVKGTVETKRKKPKPEE
                     IKTATYNAKILAIKKYASEKDSRRRELINELLPEIEKNIDNYILEVNYLNDGEYKNGI
                     WEQLMEITIDDVQLDLLINERMKGIMEMTKPTYLSFVYVAREVAAVESEGKTVKKDLS
                     AKSDVAASSSVATSGETSSDIYDSDKISTSSQADISLSKKGSANKSIMYSESVTNKSE
                     GITYRIYNPGEIDTKVTEVFNKANFEVVPTYEVGILPEKFAYDFAALNEISSSTQKEA
                     TDIAREAGLDFLAVAMLDVGREEIDPVTGGSKVYVKVNGYILDLRKKFAVKICSAGPY
                     QFSGLGSNPTVAKTNALIEAATHASQDLVDQLRVKLGS"
     gene            complement(2953..4044)
                     /locus_tag="PLW08_04485"
     CDS             complement(2953..4044)
                     /locus_tag="PLW08_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51893.1"
                     /translation="MPKHIILTLALTLGIGLLCAFQTVKYAGFCFSGSYADISKNYPY
                     TQIILQESDSPQSYLDKLFYDYFSANPELGNFTLDIEHKTKATLSLAMALNREEVAFE
                     YYEKQTKAIYNLGCTVFIMDFADLTVLQSYPINVTYIDLYNGEPDQQTIVQTLKKLYS
                     EFILKKLESNRKNIYIRSSGSLTIKVANVNFQEEALPYLGSYGTEQNLETYSSIVAQH
                     LTEALAFKMNLTVLPYSHSNLSYKMSLSFSDASIQNFTIPPSSYDIDVNVLRFVKKLY
                     KETAVERVDLYGVELEMRIYDAEFNTEYWKQIIQNGAPKQTVVGQKIGNEFYNYNEIL
                     LLTLSDKIPATLKEDKKLMKGILKKCANY"
     assembly_gap    4173..4272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4273..5084)
                     /locus_tag="PLW08_04490"
     CDS             complement(<4273..5084)
                     /locus_tag="PLW08_04490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL51894.1"
                     /translation="MQIIAKVFEFEISEQELERECAKVSLAEREKCLEGALKRLIDRC
                     LLLYKAMESGIKISDTEYENAIWELLDEEDPLKLWSTSLEQLTPQEMEKLLKQRLMIN
                     KYINSIFPQNIPITNAKIKEFYDEQKEFFLKPEQVRCSHILIRNDNEEAKAKAEQIRG
                     EIHNADDFTYFCQKYSDCPSNNVCGDLGWFPRGKMIPEIEEVAFSLKVGEISQPFLSP
                     YGYHILMKTGQSEKEYIPFEDIKDSLYARLQQIEKEYKLLRHLAELRKQYAS"
     gene            complement(5281..6162)
                     /gene="miaA"
                     /locus_tag="PLW08_04495"
     CDS             complement(5281..6162)
                     /gene="miaA"
                     /locus_tag="PLW08_04495"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPL51895.1"
                     /translation="MIPVITIEGATASGKSALAIVLAEALNTEIISADSRQVYRYLDI
                     GTAKVTKEEQKRVKHHLIDIINPDETYNAGAFVKDASLIIEKLHSEGKIPVICGGTGL
                     YIKALLKGLFFLPPLPQEIRQNLKQRLKEEGLAALYAELKSLDPLFAEKISENDTQRI
                     LRGLEVAIGTGIPLSEHWQKQKSSCKYNAFRILIDIPRPELYQRINQRIEKMLAQGLL
                     AEIENLFALGYDENSPGLNCLGYKEFLPYFKKEAGLEECILLAAQHQRNYAKRQVTWY
                     RKGNFDLVISPSGITTF"
     gene            complement(6159..8051)
                     /gene="mutL"
                     /locus_tag="PLW08_04500"
     CDS             complement(6159..8051)
                     /gene="mutL"
                     /locus_tag="PLW08_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPL51896.1"
                     /translation="MSNIHILAEDVRNKIAAGEVIERPASVVKELVENAIDAGADSII
                     VAIENGGKDLIRVIDNGCGMNADDAMLAFERHSTSKIKTVEDITHIGSLGFRGEALPS
                     IASVSKLHLVTRTAEEEMATIVEYANGKLNNVTKTSANIGTAVTVRGLFKDLPARRKF
                     LKTAAVEARHIIKYIHYQALVYPQISFRLVIDDKEKLNYIACPERKQRIEEVFGSGFF
                     ADDVIPVEGKYNDYSVSGYIFGLEDRADKLIDAQYTFINGRYIFDKTVRHSIKAAYEP
                     FILKTRAWQKGTTPPYILFLEIPPEQIDVNVHPAKLEVRFRENNLVHSLVLETLTKAL
                     RNYEDQKFASARSKFQGGVKGEKVTSLERDIFVKNIEVPRFSAYKKEFGNLFQDDLFR
                     SEPSSTSPKDMPIFNPAQEENTEQEIFPDQPNDQLKYDILLKNEEDYINPWQFHNTYI
                     FIQVEDGLVIIDQHAAHERIIYEKLIQRSEGAPAVRQKLIIPLVIDIPPYISAEIKDL
                     VEENMELLEKVGFTLKQFSGNSLVIEEIPAELTDFASGQVFIEILKQLEKEMEITTDF
                     RECLAKSIACKSAIKANHKLTRKEMLNLINQLFACQTPYFCPHGRPLMLKIPVTDFEK
                     KFKRTL"
     gene            complement(8061..9722)
                     /locus_tag="PLW08_04505"
     CDS             complement(8061..9722)
                     /locus_tag="PLW08_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPL51897.1"
                     /translation="MILDIRINGIKHTLLKLDRPCSLADIMKRTNINQSIILAIKINH
                     REYVNEDYIPDRETLIDFITYLHPEGYRIYQDSVIFIMAKALHTLMGTAHSLVVEHSI
                     ADGVFCEVFNTQNFTEADCSCLKTAMQNIVDSDLPIEKIMVKTEEALDIFSAMGRNDV
                     LKNLKYHYQENVEIYRCGKYYDCFVHPLVERTGKVKTFDIVYRSPGFILRFPSRDEKM
                     EIKPFELPHKLFAQHQEHDKWLDILRVHNIIDINKLIDNYEISEFIQVEEALHEKKIA
                     EIAADIVTKQDVKFILIAGPSSSGKTTFAKRLGVQLQACKAKPVVIGMDDYFLPRDKT
                     PRKPDGEYDFESIYAMDLEYLNMQMNQLLEGEQIELPRYDFTRGVRRHSNNFVKLAPN
                     SIIIMEGIHGLNETLTSSIAASRKVKIYVSALNQLNIDNHNRIATTDCRLLRRIIRDH
                     RYRGYSAEETLIRWQDVREGEDKNIFPYQENADYMFNSSLTYEIGVIRKHAWKLLLGV
                     SPSSSAYMEAKRLSGLIANCKDIADSLVPYNSIIREFTDGSIFRY"
     gene            9832..10785
                     /locus_tag="PLW08_04510"
     CDS             9832..10785
                     /locus_tag="PLW08_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPL51898.1"
                     /translation="MPKNTKIQHLQREVGRLFGIVFASFFFAMGYSWFLLPYNMAPGG
                     VGGISQILYHFLGIPNGVSMIILNIPLFIISFIFIGKSFGSKSLYGMFVSSIMTDLLS
                     FPALHKMGIISDLKPYTHILNGHKIYAMLAPEDILLSAIAGSVLLGLGLGLIFRFRGS
                     TGGTDIPVALIKQKANLSIGTGYFIVETGIILLVAITFKAPKLLIWGYVNLFITTKIT
                     DLASEGLPYIKGAYIISDDVEEIRLQIFTKLERGVTYLNGVSGFHQRDIKILFVVLNR
                     RQVPLLTDIVRDVDPDAFMIIMDVYDVLGYGFKSRSLNLSE"
     gene            complement(11036..11944)
                     /locus_tag="PLW08_04515"
     CDS             complement(11036..11944)
                     /locus_tag="PLW08_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPL51899.1"
                     /translation="MKNGKTIIIVIIALLALVSCGKNTVLNPDANDPTAINFGTSNTL
                     DVVTWNLRTFPEGTDLTTLKEMISALNAEVIAFQEIMDYNAFMDLAEEIPYYDACVYS
                     ATDTYRLAYLYDTRSVTVNNQYTIFENDSNPFPRPPYILDITWNNSNYFIINNHFKAY
                     GDNYIDESDSWDEEMRRRLACQKLDNYISTSLSDKKVIVLGDMNDQIAEPEDYNVFLS
                     FLNKPEEYYFADMPIALSPTYSNVSYPSSLSHIDHILITNELFADFENAGSYCKTILA
                     ENWMLSWNTYSSTISDHRPVGIRISK"
     gene            12119..15643
                     /locus_tag="PLW08_04520"
     CDS             12119..15643
                     /locus_tag="PLW08_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL51900.1"
                     /translation="MKQYIIILILLACSLLTATTLQTFYTNGSILQAHFEGLRQQPVL
                     PEEMDVEDCESANSAVITKTLAFPFEEADLQISAMVWNVFDAEGNYLYNEQKYLENAV
                     SLVQGFTFREMRGYTIRIETQITENNIIRTLKELNFSLIGSYPLQIPAFLSPAFIDAY
                     KALADNFQDSYLRDIPFSRPKMLIITHNQLTNYQQDYISWKKSIGFDVYTVNKSDIGS
                     TVQDIKNFIANHYQTYKCDYLFLWGDVNGTYAIPTNFIVSPEYATENDADDNYYTMLE
                     GDDYFPEMIAGRFSFADASEFITMMNKTIAYEKTPFMTDTNWMKRALAVAGNYADGGL
                     RPTTPISMSQWLRDKMRNFGYTQVDTVFCPPTYPGTTTIQQSINQGVQFISYRGWGDA
                     NGWHFPSFHIPDLNNTFNGAKMPIVYSIVCNTGDFANAVNPNFGEKWMRMGTMSNPGG
                     CVAFVGPSDLHTKTRFNNSIASGAFRSIMDWDVRCFGSSVLMGKIELYKNFPNDLAPN
                     QYVAFYFHVFNILSDPSLNMWVLVPNQIPETVIASGLTFQQSDSHIRINAPNLNSGIV
                     SGTKDGINFSYAPIENGYAILPIDPNVEGNLTITVSKKNYVPLVKQLTLQPDATIGIV
                     SNNLANTILAPNESYSLGLQLKNFSSQVYNNITVNLACNHPAIVIDNPNATITSLNPE
                     ATVSVNFLFITTGAIKPGAIIDFTVTLNNPSVTCLFQLVAGGAKIQVINYEGVLEIGQ
                     ANNITFTVTNLGNFTMEDIVLQIQALTPAAIAQNNPIALGSLAPNEVKQFTATITLMN
                     DVFPGNNIPLRFDATNAEGYSYFSFYAITAGTPETDDPTGPDEYGYFAYDSTDLGYPS
                     TPIYEWIELDPEGGNLLGNVFLCKDDSVKTVPLPFTFRFYGVDYNSVTMSSNGWISFI
                     PTDQSDFYNCYIPAALGPYAMVAGYWDDLKGMKTGEDESGNPIFANMRIIYWYDSANN
                     RYIIEWNKAYNQYTIDLGPNASMEKFQIILYPKLEQDGDIVIQYHTVDNPGITTNYCT
                     VGIEDHNQLRGLTYTHSNTYPVTATPLTPGLAIKFTTTWPDNYVANEDETLPVPVCNL
                     HNYPNPFNPVTTISFTAKQKGFATLNIYNLKGQLIKTLLKDNILSGEHKLIWDGKDNQ
                     GNNVASGVYLYKLNMNGSSQIKKMLLLK"
     gene            complement(15686..16603)
                     /locus_tag="PLW08_04525"
     CDS             complement(15686..16603)
                     /locus_tag="PLW08_04525"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPL51901.1"
                     /translation="MEQILNPLQTSLGRLKVNSPVTVASGTFALENLDFIEQDHLGAY
                     VCKTITRYPKKGNPPPRLYETEAGLLNSIGLQNPGLDKFIAEDLPVLRETLTVPLIVS
                     FSGSSLTEFAQMLECLEKQEGISGYEVNVSCPNVENEGIAFGIDPDVVNKLCALLSPL
                     TERELIVKLSPNVTDIAVIAKAAEEGGATAISLINTIWGMAIDYCNGKSRIKKGIGGY
                     SGIGIKPLALALTYRAAQAVKIPVIAMGGIYNWQDALEFFWAGASMIALGTANFIDPL
                     AVDKVYEGLSSFCLEKQLNLKDIVGKVNK"
     gene            complement(16588..17379)
                     /locus_tag="PLW08_04530"
     CDS             complement(16588..17379)
                     /locus_tag="PLW08_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPL51902.1"
                     /translation="MNKSQPQFLERAIYFREELSGDYFILWIWDEALGKKCQPGQFFQ
                     IRAHYSIADAEETRSLPKLFKPISIYDNAEGRIGFFIKKVGAGTNALYKLKAGDLLEL
                     IGPSGNGFPLVTGRKILLVSGGIGYPPLWYLQKKLIKHNLLYWVHGGSGQSDVFPCDE
                     VWTEDGSFGKKGMVTEGMGKLIAEKKIDLVYSCGPVPMLKECAIITTELNIEHYCSIE
                     AYMACGIGVCYGCAIPVGQENDWNYLRVCKEGPVFNAREVLWNKF"
     gene            complement(17372..18670)
                     /locus_tag="PLW08_04535"
     CDS             complement(17372..18670)
                     /locus_tag="PLW08_04535"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPL51903.1"
                     /translation="MKTLIKNANIFQNGKFTPGEILIERKKIIKISRGMASSDYTANK
                     VDKIIDAKGACVFPGFIDLHSHLRDPGQTYKEDIVTGTKAAAHGGFTAVCAMPNTEPI
                     TDNIASVEYIQLRAKDYGSAKVYVIGAITKQSAGEEIAEMATMKAGGIVAVSDDGKCV
                     QNARLMLSCMKYASNFGLPVIIHPEDYSLAGKGQIHSGKVATKLGLSGIPGLAEEVII
                     ARDIMLAESAGAKLHIAHISTARSIELVKNAKGKGLPVTCEVTPHHLVLNEEANLTFN
                     TNTKMKPPLRSEEDRLACVQALQEGIIDCIATDHSPHADFEKEREFDLAPFGIIGLET
                     AFPVLYKNLVQTEQIDLNRLIEALTIAPAKILNLPGGILAEGKTADLTIIDMEKETLF
                     SAENILSKSKNTPWLNQWFPGRVMCTICDGKITYLDSENE"
     gene            complement(18684..19622)
                     /locus_tag="PLW08_04540"
     CDS             complement(18684..19622)
                     /locus_tag="PLW08_04540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPL51904.1"
                     /translation="MASTLQFAGRSLFDLDDYSREEIMFILEAAKGMKEINLREYKKI
                     PTLRGKTVCTLFVENSTRTRMSFELAANRLSADVVSFQSSISSLQKGESLQDTVYTLN
                     AMGIDLYCIRHSSPGSPQLVNKYSGKPVINGGDGRHSHPTQALLDIFSIWEKLGDLKG
                     LKITIVGDILNSRVVRSNLIGMGKLGAKVTVCGPKTLMPGNMESVYDCRVEYDLAKAL
                     KDADVVMGLRMQLERMTEGLFPSLEEYSKHYVLSKETLKYAKRNALIMHPGPMNRGVE
                     ILPEIADSNHSIIVEQVANGVAVRMALMFLILGGKA"
     gene            19873..>21810
                     /locus_tag="PLW08_04545"
     CDS             19873..>21810
                     /locus_tag="PLW08_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPL51905.1"
                     /translation="MKRQLCVILMLLLLSSITAVALQRYKDVNRKPYATDLIKIKLSA
                     EAVSRANLPAGLYAEKSSTGINELDQLMSQTGATKIIRAHRNIKNTSWEQQTGFDRWF
                     LLKLNGKTTVGEAIKQFKANRYIETAIPEYIAYPAAVPNDPYYTNNWGHNNTAQLPGY
                     TAYGHTGAGVGTVGFDSDAQLAWNQSQSYGSANIIIAIIDSGVDTAHPDLRLVAGYDF
                     GDNDSNPMDNSAEPGHGTACSGIAAAKANNSLGITGIAGGCSVMPLKVADSDGDMYFT
                     AIENALTYAADNNAHIASMSLGATDVAEGDSPSTDAALNYAYNAGVVIFAATGNENNS
                     TISYPANQTSVISVGAASPTGQRKSTSSSDGEYWWGSNYGVATQNNKNAVDIMAPTIL
                     PATDLTGTGNGYNTSGDYYLWFNGTSCATPYAAGVAALLLSKDPSLTPAQIFSILTSS
                     ATDMTSDGGVGWDRYTGYGMVNANAALNTILGGMPSCTITSPANGATFVLNSTITINV
                     NADDINGTITSVKFYINNTLYNTDYSAPYTWDWNATGFIAGTYIIKAVATDNSNNEAT
                     NEISIFLTPPVSEAIIGTGTYITEATSASPVNVWYKSLHGQSVYTKAELNAAGIFGPV
                     NITRLGFNIVGLPLVSMPNFVV"
BASE COUNT         6364 a         4314 c         4018 g         7014 t
ORIGIN      
        1 tgcggaggaa cagcgtcctc cggctacaga tttaatgcaa attgaggaga aattggttaa
       61 attccctgcg gaatttattt tctatatttt gggcaagaac tctaattcct ctggaggttg
      121 cttcctcaat agtagaaccg ccttgttccg ttttatccac gaaggaaaag aggttttttt
      181 cgccgtaatt gaaagaaata ttcagacgca ggggttgaaa aaattgttgt cccagggatt
      241 tatgcggctc gggattcatt gtagcgacaa tttgatattc agggttatcg ttttccaaca
      301 agccaatttg attaattaca tttttaagcg aggcatctaa aaggcctgaa gaatcgccaa
      361 aaactctgat ttgcagggga atttcccgag ccgcataatt cctgagttcc aaaagttcct
      421 gtaagctgtg aaaaggtttt aacaaaatgc ctgaatcact tgccagggcg ttataataag
      481 tataagcatt ttgtgtttgt tgcaatttat caatagcgct taaaatttta gcaaagcgga
      541 taaggggatc atcattggga gcaaggcaat tttcaataaa ctcctcgcag tcgtcaattt
      601 cattgcgata aatggtctgc acggcaattt tatccattac cgctaaagca tagaaagttt
      661 tagttttagg gtcttgccag gtttctttta tctgagtata tttcaaagtt ccttctgcga
      721 ttgcttttac ggaagaagag gtctcgctgc ttgtttgata attggaagaa gagctttttt
      781 cgctttccat acttttcgta actgcaataa cgcgtgtttc aaaaaagccg gcaatatttt
      841 tcaatgcttc attttgtgca atattcagtt cttttgccat tcccacgcca caaagatatt
      901 gcttggaagg atattgggaa taaggattag aaatccattc cggttttttc tttccggcat
      961 agaggcagga accgataagc aagaataaaa ggacggtata aataattttt tttggcatta
     1021 ctttatctaa cctgtaagca atttaagcgg aatgaagaac ttgaaccatt catctaactc
     1081 aaatttgttg ttgcacccat aaacaaaaaa gacaattcac tgaaacaggg aatcggtaac
     1141 atctttcgga atttcaatat tttgggtttt ggcaatggta ataaactcac taatagtcct
     1201 catctgattt gaaagcaaaa caggaagacc aattactaca ttcgtagcat aaggaatatc
     1261 tttaacatac ataactgcgt ctattccttt agcatttttg gcttcattta cgaacttttt
     1321 ggaactttcc agaatggttt tttcccgcac aatagagctg gcaagataaa taaagccact
     1381 taaaaaatag accttttgcg attcgctcaa ttgggcttta tccgttttca ggtcttccag
     1441 tttggctttc aaggtttcag atatttcggt atcactgcct tccaggtttg cggacatcag
     1501 agattttcct tctgcttcta tttttacata ttcctgacgc atccccaaag aaacagctac
     1561 catttcttcc gctattttgg attccgcctt agccagttta tagttataat taattacttt
     1621 aaagtttgat gttttgggca tcaaaagagc gctggcaaat gcttccagtg aaagcaaagg
     1681 gagtttgtta taatattccg gattatccaa ttgagaatca tccaaaaaca attttacttc
     1741 cgcatccgct tttttctttt ttgcttccag tcccacacaa cttaccagaa gcaaaaaaat
     1801 acagatgagg acaatcaatc gctgaatagt tctcattaaa tttcttattt tagcttccaa
     1861 gttttacacg aagctgatct accaaatcct gtgaggcgtg tgttgccgct tcaattaaag
     1921 cattcgtttt agctacggta ggatttgagc ccaaaccgct gaattgataa ggtcctgcac
     1981 tgcaaatttt aacagcgaat tttttccgca ggtctaaaat atagccgttt actttcacat
     2041 agaccttact gcctcctgtt accggatcaa tttcttcccg tccaacatcc aacatagcaa
     2101 cagccaaaaa atccaaacca gcttcgcgag caatatctgt tgcttctttt tgtgtggaag
     2161 aggaaatttc gtttagagca gcaaaatcgt aggcaaattt ttccggcaaa atacccactt
     2221 cgtatgtagg aacaacttca aaattggctt tattaaagac ctccgttacc ttggtatcaa
     2281 tttccccggg gttataaatg cggtaggtaa tcccttcgct tttattggta acggattcgg
     2341 aatacattat ggatttattg gcggagccct ttttacttaa gcttatatcc gcttgactgc
     2401 ttgtgcttat tttatcgcta tcataaatat cggaggaggt ttctccgctg gttgcaacag
     2461 aactggaagc agcaacatcg gatttggcag aaaggtcttt tttaacagtt ttcccttcac
     2521 tttccactgc agcaacttct ctggctacat acacaaagct taagtaagta ggtttggtca
     2581 tttccataat gcctttcatc cgttcattga ttaaaaggtc taattgaaca tcgtcaatag
     2641 taatctccat caattgttcc cagatgccat ttttgtattc accatcgttc agataattaa
     2701 cttccagaat atagttatct atgttttttt caatttccgg cagcaattca ttgatgagtt
     2761 ctcttctacg actgtccttt tcggaagcgt atttcttaat agccagaatt tttgcattat
     2821 aagtagcggt tttaatttct tccggtttcg gctttttcct tttggtttca accgtgcctt
     2881 tcaccttaac tttaactgcc gcatccagat aagcaaagga aaagagcaaa gtaatgccga
     2941 gtaaaataat agttagtagt ttgcgcattt ttttaatatc cccttcatta gttttttatc
     3001 ttctttcagc gtagcgggta ttttatccga tagcgttaaa agaagaattt cgttgtagtt
     3061 ataaaactcg ttgccaattt tttgaccgac aacggtttgt ttcggagctc cgttttgaat
     3121 aatttgtttc cagtattcag tattaaattc tgcatcgtaa atacgcattt ccagttctac
     3181 accataaaga tcaacccttt ccaccgctgt ctctttgtat aactttttca caaagcgcaa
     3241 gacatttaca tctatatcat aactggaagg cggaatagta aaattctgaa tactggcatc
     3301 ggaaaaggaa agtgacattt tgtaggataa attactgtga gaataaggca ggacagttaa
     3361 attcattttg aaagccaatg cttcagttaa atgctgagct acaatactgg agtatgtttc
     3421 caggttttgt tccgttccat aagagcccag ataaggcaat gcttcttcct ggaaattgac
     3481 attggcaact ttaatagtta acgaaccgga ggaacgaata taaatgtttt tccggttgct
     3541 ttccagtttt ttcaggataa attcggaata gagcttcttc agggtttgaa ctatggtttg
     3601 ctggtccggt tctccattgt aaaggtctat gtaagtgaca tttataggat aggactgcaa
     3661 aacggtcaga tctgcaaaat ccataatgaa aacagtgcag ccaagattgt aaattgcctt
     3721 ggtttgtttt tcataatatt caaaggcaac ctcttcccta ttcaaagcca ttgccagaga
     3781 aagagttgcc tttgttttgt gttctatatc caaagtaaaa tttcccagtt cgggattggc
     3841 ggaaaaataa tcgtagaata gtttatccaa ataggactga ggagaatcac tctcctgcaa
     3901 aattatttgg gtgtaaggat agtttttgct tatatcggca taagaaccac taaaacaaaa
     3961 acctgcatat ttaaccgttt ggaaagcgca taaaagccct attcccaaag ttaatgctaa
     4021 tgttaatatt atgtgtttag gcatattaaa attacctctc tcccgttctg gagaataaca
     4081 tattgggcat ttatcctttt atgtttcaat acctatttct gtcaagaact ttgattgcgg
     4141 gaaaaaagaa gttataaagc tataaagtta tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nntgggatgc atattgtttg cgtaattcag ccaaatgcct gagcaacttg
     4321 tattctttct caatttgttg taagcgggca taaagcgaat ccttaatatc ttcaaaaggg
     4381 atgtattcct tttcgctttg ccctgttttc attaaaatgt gataaccata aggagacagg
     4441 aagggttggc tgatttcacc cactttaaga gaaaaagcaa cttcttcaat ttcgggaatc
     4501 attttgcccc ggggaaacca acccaaatcg ccacaaacat tgttacttgg gcaatcggaa
     4561 tacttttggc aaaaataggt aaagtcatca gcattatgaa tttccccacg aatttgttcc
     4621 gctttggctt ttgcttcttc attatcgtta cgaataagaa tatgagagca acgcacctgt
     4681 tccggtttca ggaaaaattc cttttgttca tcataaaact ctttaatttt ggcattggtg
     4741 atggggatat tttgcgggaa gatagaattg atgtatttgt taatcatcaa ccgctgtttc
     4801 aaaagttttt ccatctcctg gggtgtgagt tgttctaaag aagtgctcca gagttttaaa
     4861 gggtcttctt catccagaag ttcccatata gcattttcgt attcggtatc gctaatttta
     4921 atgccgcttt ccattgcttt ataaagcaaa aggcatctat caatcaagcg tttaagagca
     4981 ccttccagac atttttcgcg ttctgccaaa cttactttag cacattcgcg ttccagttct
     5041 tgctcgctta tttcaaattc aaatactttg gctattattt gcatttacca cctcagtttt
     5101 gatcatcctt tatccttgag attacagttg ataatttaat caaagaagag gcagttgtca
     5161 aatcaaaaaa aaacaggaag gttgatatcc tcatcaacct actttttgtg tagtcagagc
     5221 ttgctgaagc tccgcttttt tctttaatga gaacgacggg gatatcgttt ttccggaata
     5281 ttaaaaagta gtaattccag acggagaaat aactaaatca aaattgcctt tccgatacca
     5341 ggtaacctgt cttttggcat aattgcgttg atgctgagca gccaacagaa tacattcctc
     5401 caaacctgcc tcttttttga agtatggcaa aaattcttta taaccgagac aattgagacc
     5461 tggtgaattt tcatcgtaac ctaaagcaaa aaggttttca atttcagcca gtaaaccttg
     5521 agcaagcatt ttttctatgc gctgattgat gcgctggtaa agttccggac gaggaatatc
     5581 aattaaaata cgaaaagcat tatatttaca gctgctcttt tgtttttgcc agtgttcgga
     5641 aaggggaatt ccggttccta ttgcaacttc caatcctctc aagattcttt gggtatcatt
     5701 ttcgcttatt ttctccgcaa acagagggtc taaacttttc aattcggcat ataaagctgc
     5761 aagtccttct tctttaaggc gttgtttcaa attttgtctg atttcctgag gcaggggagg
     5821 aagaaaaaaa agccctttta agagagcttt tatatataag cccgtgcccc cgcaaataac
     5881 aggaatttta ccctcggaat gcaatttttc aattatcaga gaggcatctt taacaaaagc
     5941 cccagcatta tatgtctcat cgggatttat aatatcaatt aaatgatgtt taactctttt
     6001 ttgttcttct ttagttactt tggcagttcc gatatccaga tagcggtaaa cctgacggga
     6061 atcggcagaa ataatttctg tgtttaatgc ttctgccaaa acaatagcca atgcggattt
     6121 tccggaagcc gttgctccct caatagtgat aacgggaatc ataaagtgcg tttgaacttt
     6181 ttctcaaagt cggttaccgg aatttttagc atcagaggtc tgccatgagg acagaaatag
     6241 ggtgtttgac aggcaaaaag ctgattgatt aaattcagca tttctttgcg ggtaagctta
     6301 tgattggctt ttatagcgga tttacaggca attgactttg ccaggcactc tctgaaatca
     6361 gtagtaattt ccatttcctt ttccagctgc tttaaaattt caataaagac ctgaccgctg
     6421 gcaaaatcag tcaattcagc tggaatttct tctattacca gtgagtttcc actaaattgt
     6481 ttaagggtga aacccacttt ttccaaaagt tccatatttt cttccaccag gtctttaatc
     6541 tcggcagaaa tatagggtgg gatgtcaata actaagggaa taataagttt ttgacgcaca
     6601 gctggagctc cttcactgcg ttgaatcaat ttctcataaa ttattctttc gtgagctgca
     6661 tgctggtcaa taatcaccaa gccgtcctca acctggataa aaatataggt attgtgaaac
     6721 tgccagggat tgatatagtc ctcttcattt ttcagcagga tatcgtattt cagttgatca
     6781 ttgggctggt caggaaaaat ctcctgctct gtgttttctt cttgagcagg gttaaaaatg
     6841 ggcatatctt ttggggaagt ggaacttggc tcactacgaa aaaggtcatc ctgaaagaga
     6901 tttccgaatt cctttttgta ggcggaaaaa cgcggaactt ctatattttt aacaaagata
     6961 tcgcgttcca gggaagttac tttttctcct tttactcccc cttgaaattt actgcgtgca
     7021 ctggcgaact tttggtcttc ataattgcgg agtgctttgg taagcgtttc caaaaccaaa
     7081 gaatgcacca aattattttc ccgaaaacgg acttccagtt tggcaggatg aacattcaca
     7141 tcaatttgtt ccgggggaat ttccaaaaaa agaatatagg gaggtgtagt tcctttttgc
     7201 caggcgcggg tttttaaaat aaaaggttca taggcggctt taatgctgtg acgcactgtt
     7261 ttatcaaaaa tatagcgtcc gttgataaaa gtgtattgtg catcaatcag tttatctgct
     7321 ctatcttcca agccaaagat ataaccgctt acggaataat cgttatattt tccttctacg
     7381 ggaataacat catctgcaaa aaaaccactg ccaaagacct cttcaatacg ctgttttctt
     7441 tctgggcagg caatgtaatt cagcttttcc ttatcgtcta tcaccaaccg aaaactgatt
     7501 tggggataaa ccaatgcttg ataatgaata tatttaataa tgtgcctggc ttccactgct
     7561 gccgttttta aaaatttcct tcttgctgga aggtctttga ataatccgcg cacagttact
     7621 gcagttccga tgttggcaga ggtcttagtt acattattta atttgccgtt agcatattct
     7681 acaatcgtag ccatttcttc ctcagcagtt ctggtgacca aatgtaattt ggaaacggag
     7741 gcaattgagg gtaatgcttc acctctaaaa cccagggaac cgatatgggt aatatcttcc
     7801 accgtcttta ttttgctggt agaatggcgt tcaaaagcca gcattgcatc atcagcattc
     7861 attccacaac cgttatctat aaccctgatc aggtctttac caccgttttc tatagctaca
     7921 attatactat cagcaccggc atcaatggca ttttctacca gttctttaac cactgaagca
     7981 gggcgttcaa ttacttctcc tgcagcaatt ttattacgga catcttctgc caaaatatgg
     8041 atattagaca ctttaaaacc tcaataacgg aagatactgc cgtccgtaaa ttctctgata
     8101 atagaattat agggcaccaa ggaatcggca atatctttac aattggcaat taaaccggaa
     8161 agacgcttag cttccatata ggcagaagag gaaggagaaa cgcctaaaag caatttccag
     8221 gcatgtttac gaattactcc aatctcgtaa gttaaactgc tattgaacat ataatccgca
     8281 ttttcctgat agggaaaaat gtttttatct tcaccttcgc gcacatcttg ccagcgtatt
     8341 aatgtttctt cagcgctata acctctataa cgatggtcgc gaataattct gcgcagcaat
     8401 ctgcaatcgg ttgtagcaat tcggttatga ttatctatat tcaattgatt aagagcgcta
     8461 acataaattt tcactttcct gctggcagca atggaagaag ttaatgtttc attcaaaccg
     8521 tgaatacctt ccataataat tatactgtta ggggcaagtt ttacaaaatt attgctgtga
     8581 cgacgcacac ctctggtaaa atcataacgg ggcaattcaa tctgttcgcc ttccagcaat
     8641 tggttcattt gcatattcaa atattccagg tccatagcat aaatggactc aaaatcgtat
     8701 tcaccatcgg gtttgcgagg agttttatcc cggggcaaaa agtaatcatc cattccaata
     8761 actaccggct tggctttaca tgcctgaagc tgaactccta atcttttggc aaaagtggtt
     8821 ttgccggaag aagaaggacc cgctatcaaa ataaatttca catcttgttt ggtaactata
     8881 tcggcagcaa tttcggctat ttttttctcg tgtaatgctt cttccacctg aataaattcc
     8941 gaaatttcgt aattatcaat caatttattg atatctataa tgttatggac gcgtaaaata
     9001 tccagccatt tatcgtgttc ctgatgctgg gcaaagagtt tatgcggaag ttcaaagggt
     9061 tttatttcca tcttttcatc ccggctggga aagcgtaaaa taaagccagg agagcgataa
     9121 acaatatcaa aggtctttac ttttcctgtt ctttccacca aaggatgaac aaaacaatcg
     9181 taatattttc cgcaacgata aatttccaca ttttcctgat agtgatattt caaattttta
     9241 agcacatcat tgcgacccat agcactaaag atatccaatg cttcttcggt tttaaccata
     9301 attttttcaa tcggcaaatc gctatccaca atattctgca ttgctgtttt gaggcaactg
     9361 caatccgctt ccgtaaaatt ttgcgtgttg aaaacctcac agaaaacacc gtcggcaatg
     9421 gaatgttcaa ctaccagaga gtgcgctgtt cccatcaaag tatgcaatgc cttagccata
     9481 ataaaaataa cgctatcctg ataaattcta tagccctcgg gatgaaggta ggtaataaaa
     9541 tctatcaatg tttccctgtc tggaatgtaa tcttcattga catattcccg atgattaatt
     9601 ttaatggcta atataatgga ttgatttata ttcgttcttt tcattatatc tgccagtgaa
     9661 cagggtctat ccagtttcag cagagtgtgt ttgattccgt ttatgcgaat gtctaaaatc
     9721 atcttttgtt tcctgttgtt ccctcaaata atggggatgt ataatgttat tttttcgtaa
     9781 accaaagatt taatgattaa gatttagtca agaatttagg agagatagaa aatgccaaag
     9841 aatactaaaa tacagcatct gcaaagagaa gtaggacgct tgttcggaat cgtctttgct
     9901 tccttttttt tcgctatggg ttattcctgg tttttattac cctataatat ggctcctgga
     9961 ggggtaggag gtatttcaca gattttatat cactttttgg gaattcccaa tggtgtttca
    10021 atgattattt tgaatatccc attgtttatc attagtttta tctttatcgg caaatccttc
    10081 ggtagcaaaa gcttgtatgg tatgtttgta agttcaatta tgactgacct tttaagtttt
    10141 cctgctttac ataaaatggg aattatttct gatttaaagc cctatacaca tattcttaac
    10201 ggtcataaaa tctacgctat gcttgctccg gaagatatac ttttaagtgc aattgccggt
    10261 agtgtattat tgggattggg tttaggactg atttttcgtt ttcggggttc tacaggggga
    10321 acagatattc ctgtagcact cattaaacaa aaagcaaacc tttctattgg aacgggatac
    10381 tttattgtgg aaacaggaat tatccttctg gtagctatta cctttaaagc accaaaactt
    10441 cttatctggg gttatgtaaa tctatttatt acaaccaaaa taaccgattt agcaagtgaa
    10501 ggtttacctt atattaaagg tgcttatatc atttccgatg atgtagagga aattcgcctg
    10561 caaatattta ctaaactgga acgcggagtt acctatctca atggagttag cggttttcat
    10621 cagagagata taaaaatact ttttgtggtg ttgaaccgtc gtcaagtgcc tttgcttaca
    10681 gatatagtaa gagatgttga tcctgatgct tttatgatta ttatggatgt gtatgatgtt
    10741 ctgggatatg gctttaagtc acgcagttta aatttgagtg agtgattttt aattgtattg
    10801 catactcatt gcttacccat tgctaatgcc atcagtaatg ggtaagcaat gagtaagcaa
    10861 tgctacttgg caaaagagaa tgaaaaatga agctattaat aaagaagaag ggataacatt
    10921 tgctgtcttt aggaaagaat tgtaaaatat aaatctgtgt tatcagtgtt atctgtgaga
    10981 gttaatctat attatctgga agagttaatc cgtaaaatcc gtttaatcta tattgctatt
    11041 tggatatacg aatccccacg ggtctatgat ctgaaattgt agaagaatag gtattccagc
    11101 tcagcatcca gttctctgcc aaaatcgttt tacaataact tcctgcattt tcaaaatccg
    11161 caaaaagttc attggtaatc aaaatatggt ctatatggct gagtgaactt ggataagaaa
    11221 cattggaata ggttggagaa agagcaatcg gcatatcggc aaagtaatat tcctccggtt
    11281 tattcagaaa actaaggaaa acattatagt cctccggttc ggcaatctga tcgttcatat
    11341 cacccaaaac aatcactttt ttatccgata gagaggtaga aatatagtta tccagtttct
    11401 gacaggcaag acgacgacgc atttcttcgt cccagctgtc actttcgtca atataattat
    11461 ctccgtatgc tttaaagtga ttgtttataa taaaatagtt actgttattc caggttatat
    11521 ccaaaatgta tggtggacgc ggaaaaggat tgctgtcatt ctcaaaaata gtgtattgat
    11581 tattaacggt aactgacctt gtatcataca aataggcaag acgataagta tccgtggcag
    11641 aataaacaca agcgtcataa tagggaattt cttccgccaa atccataaag gcattataat
    11701 ccataatttc ctgaaatgct ataacttccg cattcaaagc ggaaatcatt tctttaagag
    11761 tagttaaatc cgtcccttcc ggaaaggttc ttaaattcca ggtaactaca tccagcgtgt
    11821 ttgaagttcc gaaattgatt gctgtaggat cattagcatc aggattcaga actgtgtttt
    11881 tcccgcagga gaccaacgcc aacaacgcta ttattacaat tattatcgtt tttccgttct
    11941 tcatttattg ttccttaaac ttccaaatat ctaggttttc aagcccaact tcggaaggag
    12001 tatataataa tgcaattctt ttgccaattt ccgtaaaata tttgagagca aaaattatta
    12061 ccgaggttgg aagggttatt gcattataca ttaactacac aatataggtg gaggataaat
    12121 gaagcaatat ataataatcc tgattctgct tgcctgcagt ttgttaacag ctacaacact
    12181 gcagaccttt tatactaatg gttctatttt acaggcacat tttgagggct tgagacaaca
    12241 gccggttttg cctgaagaaa tggatgttga agattgtgaa tctgctaata gcgctgttat
    12301 cactaaaact cttgcctttc cttttgaaga agcggatttg caaatttcgg ctatggtctg
    12361 gaatgttttt gatgcggaag gcaattattt atataatgaa cagaagtatt tagaaaacgc
    12421 tgtttctctt gtgcagggtt tcacttttag agaaatgcgc ggttatacga ttcggataga
    12481 aactcaaata acggaaaaca atataatccg gactttaaag gaactcaatt tttccttaat
    12541 cggttcttat ccgcttcaaa ttcctgcctt tctttccccc gcttttattg atgcctacaa
    12601 agctttggca gataattttc aagattccta tttgcgggat atacccttca gccgtcctaa
    12661 aatgctgatt attactcaca accaattaac aaactaccaa caggactata tttcctggaa
    12721 aaaatcaatc ggttttgatg tttatacggt aaataaatca gatatcggtt ccaccgttca
    12781 ggacatcaag aatttcatag ccaatcatta tcagacctat aaatgcgact atcttttcct
    12841 gtggggtgat gtgaatggaa cttatgccat tcctacgaat tttatagttt ctcctgaata
    12901 tgctacagaa aacgatgcgg atgataatta ttacacaatg ctggaaggag atgattactt
    12961 tccggaaatg attgcgggca gattttcctt tgccgatgcc agcgaattta ttacaatgat
    13021 gaataaaacc attgcttacg aaaaaacccc ttttatgaca gatactaatt ggatgaaaag
    13081 agctctcgct gttgccggaa attatgctga tgggggttta agacctacta ctcctatctc
    13141 tatgtctcaa tggttgagag ataaaatgcg taattttggc tatactcaag ttgatactgt
    13201 tttttgtcct ccaacttatc ctggaacaac tactattcaa cagtccatca atcaaggtgt
    13261 ccaatttatc agttatcgtg gttggggtga tgctaacggt tggcattttc cttcttttca
    13321 cattcccgat ttgaacaata cttttaacgg tgctaaaatg ccgattgtgt attccattgt
    13381 ttgtaataca ggtgattttg ccaatgctgt aaacccaaat ttcggtgaaa aatggatgcg
    13441 tatgggaaca atgtccaatc cgggaggttg tgttgctttt gtgggtcctt ccgatttaca
    13501 tactaaaacc cgatttaata attctattgc cagtggagct tttcgcagta ttatggattg
    13561 ggatgtgcgt tgtttcggtt ccagtgtttt gatgggtaaa attgaactct acaagaactt
    13621 ccctaatgac cttgccccca atcagtatgt tgctttttac tttcatgtgt ttaacatttt
    13681 aagtgatccc agtttaaata tgtgggtttt agtgcccaat cagattccag aaaccgtaat
    13741 tgccagtgga cttacttttc agcaaagtga cagtcatatc agaataaatg cccctaactt
    13801 gaatagcggt atagtatccg gaaccaaaga cggtatcaac ttcagttatg ctcctattga
    13861 aaatggttat gctattttac ctatagaccc taatgtggaa ggcaatttaa ccataaccgt
    13921 cagcaaaaag aattatgttc ctttggtgaa gcaactaact ttgcaaccgg atgccactat
    13981 cggtattgtt tctaataatt tagctaatac tattctggct ccgaatgaat catattctct
    14041 tggcttacag ctaaaaaatt tctccagcca ggtgtataat aatataactg ttaatcttgc
    14101 ctgtaatcat ccagctattg tgattgataa tcctaatgct actatcactt ctctgaatcc
    14161 ggaagcaact gtgtctgtaa atttcctttt tattaccact ggagctatta aacctggtgc
    14221 aataattgat tttaccgtta ctttgaataa tccttccgtt acttgtttgt ttcagttagt
    14281 tgctggtgga gctaaaattc aggtaatcaa ttatgaaggg gttttagaaa taggacaggc
    14341 aaataatatt actttcacgg ttaccaatct gggcaatttt acgatggaag atattgtctt
    14401 acaaatacaa gcacttacac ctgctgccat tgctcaaaac aaccctattg ctcttggttc
    14461 tctggctccc aatgaagtta aacagtttac agcaacaata actttgatga atgatgtctt
    14521 ccccggaaat aatatccctc tgcgttttga tgccactaat gcagagggct acagttactt
    14581 tagtttttat gcaattacgg ctggcactcc tgaaactgat gatcctacag gacctgatga
    14641 atatggttat tttgcttatg attctaccga tctcggttat ccttcaactc ccatttatga
    14701 atggattgag cttgatcctg aaggaggcaa cttattgggt aatgtcttct tatgtaagga
    14761 tgattctgtg aaaacggttc ctctgccttt cactttccgt ttttacggtg ttgattataa
    14821 tagcgttaca atgtcttcca acggttggat ttcttttatt cctacggatc aatccgattt
    14881 ctataattgt tacattcctg ctgctttagg tccttatgct atggttgccg gttattggga
    14941 tgatcttaaa ggaatgaaaa caggtgagga tgaatcgggt aatcctattt ttgctaatat
    15001 gcgtattatc tattggtatg attccgccaa taatcgttat atcattgaat ggaacaaagc
    15061 atacaatcaa tatactatag atttgggtcc caatgcttct atggaaaaat tccagattat
    15121 cctctatccg aaactggaac aagacggcga tatcgtaatc caatatcata ccgttgataa
    15181 tcctggaata actaccaact attgcactgt aggaattgaa gaccataatc aattacgcgg
    15241 tttaacttac acccatagta atacttatcc tgtaacggca acgcctttaa ctccaggttt
    15301 agctattaaa ttcactacta cctggcctga taattatgtt gccaatgaag atgagacctt
    15361 gcctgttccc gtatgtaact tacataacta tcccaatcct tttaatccgg ttacaacgat
    15421 atcctttacc gcgaaacaaa aagggtttgc taccctcaat atttataact tgaaaggaca
    15481 gctaattaaa accctgctaa aggataatat tttatccggt gaacataaac tgatttggga
    15541 cggaaaagat aatcagggaa ataatgttgc ctccggggtt tatctctata aactgaatat
    15601 gaatggcagt tcccaaatca aaaagatgct gctactgaaa tagatgaacg ggtgaacagt
    15661 gaaaggtgaa ttcacccttc actgttcact tatttacttt cccaactata tctttcaggt
    15721 taagttgttt ttccagacaa aaagaggata atccctcata aaccttatcc acggctaaag
    15781 ggtctataaa atttgctgtt cccaaagcaa tcatagatgc cccagcccaa aagaactcca
    15841 aagcatcctg ccaattataa attcccccca tagcaataac aggaatttta acagcttgag
    15901 cagcacgata agttaaagcc aaagctaagg gtttaattcc gattccgcta tagccaccga
    15961 ttcctttttt aatccttgat tttccattac aatagtcaat tgccattccc caaatagtat
    16021 tgattaatga tattgcagta gcacctcctt cttcagcagc tttggcaata acagcaatat
    16081 ccgtaacatt gggacttaat tttactatca attctctttc cgttaaaggt gaaagaagtg
    16141 cacatagttt atttacaaca tcgggatcaa ttccgaaagc tataccttca ttttcaacat
    16201 tgggacagga tacattcact tcgtagccgg aaattccctc ttgtttttcc aaacattcca
    16261 gcatttgagc aaattcagtt aaagaagaac cggaaaaact tacaattaaa ggaacagtaa
    16321 gagtttcacg caaaacaggc aagtcctcag caataaactt atccaacccg ggattttgta
    16381 aaccgatgga attcaggagt cctgcttccg tttcatataa acgcggaggc ggatttcctt
    16441 ttttcggata gcgggtaata gttttacaaa cataagcacc taaatgatcc tgctcaataa
    16501 aatccaggtt ttccaaagcg aaagtgccgg aagcaacagt aacaggacta ttcactttaa
    16561 gccgtcccaa agaggtctgc agcggattta gaatttgttc cataatacct ccctggcatt
    16621 aaaaacagga ccttctttgc agacccgtaa ataattccag tcgttttctt gaccaacagg
    16681 aatggcacaa ccgtagcaaa cacctattcc gcaagccata tatgcttcta ttgaacaata
    16741 atgttcaata ttcagttcgg tagttataat tgcacattcc ttcagcattg gaacaggacc
    16801 gcaactatat accaaatcaa tcttcttttc tgcaatcaat tttcccattc cttctgtaac
    16861 cattcctttt ttaccaaaac tgccatcttc tgtccagacc tcatcacagg gaaaaacatc
    16921 actttgtcca gaaccgccat gcacccaata aagcaaatta tgtttaatta atttcttttg
    16981 caaataccac aaaggaggat agccaatacc tccactaacc agtaatattt ttctccccgt
    17041 aactaaagga aaaccgtttc ccgaaggacc tataagctct aaaaggtcac cggctttcag
    17101 tttatagaga gcatttgttc cggcacccac ttttttaata aagaaaccaa ttctcccttc
    17161 agcattgtca taaatggaaa tgggtttaaa gagtttaggc agagagcgtg tttcttccgc
    17221 atcggcaata ctatagtgag ctctgatttg gaagaactgt cccggctggc atttttttcc
    17281 caatgcctca tcccaaatcc acagaataaa atagtccccg gagagttctt ccctaaagta
    17341 tattgctctt tccagaaatt gtggctggga tttattcatt ttcactatcc agataagtta
    17401 ttttgccatc acagatagta cacatcaccc ttccgggaaa ccattgattg agccagggag
    17461 tatttttgct tttagaaagt atattttccg cggaaaaaag agtttccttt tccatgtcta
    17521 taatagttaa atcggctgtt tttccttcgg ctaaaattcc cccgggcaaa tttaggattt
    17581 tggctggagc aattgttaat gcttcaatta aacggttgag gtctatctgc tctgtctgca
    17641 ccaggttttt atagagcaca ggaaatgctg tttccaagcc aattatgcca aagggagcca
    17701 agtcaaattc cctttctttt tcaaaatccg catggggaga atggtctgta gcaatgcaat
    17761 caattattcc ttcttgcaat gcctgaacac aagccagacg gtcttcttca ctccgcagag
    17821 gaggtttcat tttagtatta gtattaaaag ttaaatttgc ctcttcattc agcaccaaat
    17881 gatgaggcgt tacttcacag gtaacaggaa gtcctttacc tttagcattt tttaccagtt
    17941 ctatagagcg cgccgtagaa atatgggcaa tatgtaattt agctccggca ctttcagcga
    18001 gcataatatc ccgggcaatt atcacttctt ctgccagacc gggaattcca ctaagtccaa
    18061 gtttggtagc aactttacca gaatgtatct gtccctttcc ggctaaggaa taatcttcag
    18121 ggtgaataat taccggcaaa ccaaagttac tggcatattt catacaggaa agcattaatc
    18181 gtgcattctg cacacattta ccgtcatcgg aaacagctac aatacctcct gctttcattg
    18241 ttgccatttc agcaatttct tcaccggctg actgtttcgt aatagctcca attacataga
    18301 ccttggcaga gccataatct ttagcccgta attggatata ttctacagaa gcaatattat
    18361 cggtaatagg ttcagtattc ggcattgcgc aaacagctgt aaatccacca tgcgcggcag
    18421 ctttagttcc tgttacaata tcttctttat aggtttgtcc cgggtcgcgt aaatgagaat
    18481 gcaggtctat aaaaccagga aacacacaag cacctttggc atctataatt ttatccactt
    18541 tgtttgctgt gtagtcagag gacgccattc ctctgcttat ttttataatc ttctttctct
    18601 caattaagat ttcccctggg gtgaacttcc cattttgaaa gatattggca tttttaatta
    18661 aggtcttcat tgttttttcc tccttatgct tttccaccta agattaaaaa catcaaagcc
    18721 atcctaacag caactccatt ggcaacttgt tccacaatga tactatgatt gctatctgca
    18781 atttccggta gaatttccac tcctctgttc ataggaccgg gatgcataat taaagcattc
    18841 cttttggcat atttcaaggt ttctttggag agaacatagt gtttgctgta ttcttccaga
    18901 gaggggaaaa gcccttccgt catccgttcc aactgcattc ttaagcccat taccacatct
    18961 gcatctttta aagcttttgc taaatcatat tctacacggc agtcataaac actttccata
    19021 tttcctggca tcaaggtttt aggtccgcaa acagttactt tggcacccag ttttcccatt
    19081 ccaattaaat tactacgcac tacgcgacta tttaaaatgt cgcctacaat ggttattttc
    19141 agccctttca ggtcacctaa tttttcccag atagaaaaaa tatccagtag tgcctgggtg
    19201 ggatgtgagt gtcttccatc tcctccattg ataactggtt taccggaata tttatttacc
    19261 agttgcggac ttccgggact gctgtgacga atgcagtaaa ggtctatacc cattgcattt
    19321 aaagtgtaaa ccgtatcctg taaactttct cctttctgca gagaggaaat agaggactga
    19381 aaactgacaa catctgcact taaacgattg gcagccaatt caaaactcat tcgagtgcgg
    19441 gtactgtttt ctacgaataa agtgcacact gtttttcctc ttaaggtagg aatcttttta
    19501 tattcccgta agttgatttc tttcattccc ttggctgctt ccagaatgaa cataatttct
    19561 tcccgggaat aatcatccag gtcaaacaga gagcgtcctg caaattgtag tgttgaggcc
    19621 atttttactc ctttccagct cactggctgt gattaaaggt tttttccctt ttattagaaa
    19681 tcccaatttg gtcaagggaa ttttttaatt atattataag gtaattacat tttctgtgta
    19741 atggagttca aaagagcgaa taataaagct tatattgaaa taaccgagtt tcaggtaaag
    19801 aaattgcttg cctaaattga gggcttttgt aaaaaaggaa gttaggtatc ctgatttttc
    19861 aaggagagaa aaatgaaaag acagttatgt gttattctaa tgttattact gcttagttcc
    19921 ataaccgccg ttgctttaca acgatataag gatgtaaaca ggaaacctta tgcaacggac
    19981 ctaataaaaa taaagcttag tgctgaagca gtaagtagag caaatcttcc tgcgggactt
    20041 tatgctgaaa aatcatcaac cggtattaat gaattggatc agctaatgtc ccaaaccggg
    20101 gctacaaaaa taatccgggc tcaccgcaac ataaaaaaca ccagttggga acagcaaact
    20161 ggttttgatc gctggttttt actgaaactg aatggcaaaa caaccgttgg agaagctatt
    20221 aaacaattta aagccaatcg ttacatagaa acagccattc ctgaatatat tgcctatcct
    20281 gcagcggttc ccaatgatcc ctattatacc aataactggg gtcataataa tacagctcaa
    20341 cttcctggtt atacagctta tggtcatacc ggtgcaggag ttggcactgt cggttttgac
    20401 agcgatgctc agcttgcctg gaatcaaagt cagagttatg gttcagccaa tattataata
    20461 gcgattattg attccggagt agatactgcg catccggatt tacgtttagt agccggttat
    20521 gattttggtg ataatgacag taatcctatg gacaacagtg cagaaccagg acatggaact
    20581 gcctgttctg gaattgctgc agccaaagca aataactctt taggcattac aggaattgcc
    20641 ggtggctgta gtgttatgcc tttaaaagta gccgattccg atggggatat gtattttaca
    20701 gccatagaga atgcgttaac ttatgcggct gataataatg cccatattgc cagtatgagt
    20761 ttgggggcaa cagatgtagc agaaggagat tctccttcca ccgatgctgc cttaaattat
    20821 gcttataatg ccggtgtagt tatttttgcg gctacaggca acgaaaataa ttctacaatt
    20881 tcgtatcctg ccaatcaaac atctgtaatc agtgttggcg ctgccagccc tacaggtcag
    20941 cgaaaaagta cctcctcttc cgatggagaa tattggtggg gttccaatta cggagtagct
    21001 actcagaaca ataaaaatgc tgtagatatt atggcaccga caattttacc tgctaccgat
    21061 ctaacgggaa caggaaatgg ttataatacc agcggggatt attatttgtg gtttaacgga
    21121 acttcctgtg ccacacctta tgcagcggga gttgctgcgt tactgctttc caaagaccct
    21181 tctttaactc ctgctcagat tttttccata cttacttcat ctgctaccga tatgacatct
    21241 gacggaggag ttggttggga tagatatacc ggttatggaa tggtaaatgc caatgcagct
    21301 ttaaatacca ttctgggtgg aatgccttcc tgcacaatta cctcccctgc aaatggtgct
    21361 acttttgttc ttaattctac catcactatc aatgtaaatg ctgatgacat caatggcaca
    21421 attacttccg tgaagtttta catcaataac actctttata atacggatta ttctgctcct
    21481 tatacttggg actggaatgc taccggattc atcgctggaa cctatatcat taaagcagta
    21541 gcaacggata atagtaacaa tgaggcaacc aatgaaatct ctatattttt aactccacct
    21601 gtttcagaag ctattattgg cacaggaacc tatattaccg aagctacaag cgcatcacct
    21661 gttaatgtat ggtataaaag tttacacggt caatccgttt atactaaggc agaattaaat
    21721 gctgctggta tcttcggtcc tgtaaatatt acccggcttg gttttaatat agttggttta
    21781 cccttagttt caatgcctaa tttcgtcgtc
//
LOCUS       DAOOZV010000032        21068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2637_length_21068_cov_64.346881, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000032 DAOOZV010000000
VERSION     DAOOZV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_21068_cov_64.346881"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLW08_04550"
     CDS             complement(<1..1142)
                     /locus_tag="PLW08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPL51906.1"
                     /translation="MKYSYLKQWTQEMIGYQAVIESVLLFPELLVLQTKDKKALCIVL
                     SKRDAFIFLQDNFQLPQEGKKIWQKLNNCHLTGISLAENDRLAYLELQQRDIYQQNKK
                     YYLIAELMTPQPNAILTDSELHILDALHKYSYADNPQRQILPGLVYTAPKTSFQPILE
                     EVKSPYPDGSATCNDYFINLYYNKLKQEEEAENSQKICTALKKELQKLQKKREILQQD
                     LTNAEKADFWLACAECLKTNLHNLKTGRESFTAINYLDPDLKEIEILLQKDKSPQENL
                     QSYLKRYHKAKNGLQIITKNLAQTDAEIEKIKDLIAKAEKGELPDSGILPSKPTGHKI
                     VQNAILADKILKLKINDEFQILIGRRAKENDYLTTQLARPYDYWFH"
     gene            1227..2099
                     /locus_tag="PLW08_04555"
     CDS             1227..2099
                     /locus_tag="PLW08_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51907.1"
                     /translation="MKATTAIIVAVLAILTIVFAVLWINANGKSKDIMKSNEELKKLY
                     ETSTSTINEIQSSLESLEQDLSGQLFTQKEIPGTSPEERRNRIISSIANMRDQIEADK
                     KKIATLEKQLATSKSQLKGVQEIVNKLKASISEKEQIMDELQQRLGILNETLETERRT
                     SAEEIQKREMTITEKEQQITQQSIEVNTIYYVYGTRKELMSKGIMDRKGGLLGIGKVS
                     TVKQNIPIEQFTSMNLLETQQISFPATKKGYSILTNHPATSYKVEKEGDQNILTILDA
                     TSFRKQKFVVIELL"
     gene            complement(2175..2690)
                     /locus_tag="PLW08_04560"
     CDS             complement(2175..2690)
                     /locus_tag="PLW08_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPL51908.1"
                     /translation="MPDSLNTIAQPIVATMASQNLAYFLAWIGLFLMVALSGMGSAWG
                     TVIGGRASVGAMKKRDDIFPSCMILSALPGTQGLYGFGSFFILKAYITPQINMLQACA
                     ILGAGLISGTVELISAYHQSRIVATGIESIGSGHDVFAKTLILGVFPELYAIIAFAAS
                     FLIGGVIPTLA"
     gene            complement(2705..4492)
                     /locus_tag="PLW08_04565"
     CDS             complement(2705..4492)
                     /locus_tag="PLW08_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPL51909.1"
                     /translation="MIEKMRKYTFVLYHQDYENFLAELQKLGVVHIIRSCDEKTESQT
                     KNLELMKEYGECVKFLTKLKSNAPKQASPLPTKALLNKINEAREEKDKLQHSIDTVKK
                     QIRDLSPWGHFDYDLVRKLKEYGLTIRFYTCLKNHFKPEWQEKYPLKIINEVGGILYF
                     VVITDNGDPALEADTFSFHQHTLQEFEDKLKELENEQKDIDDYLYSIAPTAIEMFQDE
                     LKRLSREYEFEDALQQGKYEAENTIRLLSGWIPVSLEQGLKDFLEKNNIIYVSEDAKP
                     EENPPIKLRNNWFARLFEPISKMYMLPYYDEFDLTPFFAPFFALFFGFCNADIGYGIV
                     IIILAVILKLKMKNQGMRDILTLLIIFGTASIIMGWVMGSALAFDLKTIPAIQDKILI
                     KDNNQIFNYALLLGAIQILFGIAIAAVKKMKKNGFKFGLSTIGTWLLLFGLTVLGSSL
                     LGANISKVQPYIKYPIYIGLAMILLFNSPGKNIILNILNGFWLLYQIVTGYFGDILSY
                     IRLFALSVSSAILGFVMNSIGSQIANGIPIVGPIIFVLFMLFGHTLNIALGGLSGFVH
                     PLRLTFVEFYKNAAFAGPGLEYKPFGKKE"
     gene            complement(4489..5088)
                     /locus_tag="PLW08_04570"
     CDS             complement(4489..5088)
                     /locus_tag="PLW08_04570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPL51910.1"
                     /translation="MNLKFQYNKISQLQLIKQLAVRQRALPTLKNKESALRLEVKKAR
                     DQAFALDQKVKERTAELDAFMKLFVEFDPDIVKIKNVEIKTRKIAGVKTPLLENIEFE
                     IADYNLFKAPSWYPEGIALLKEICQLQIERDFYIRKMQILEQVRKKTTQKVNLYEKVQ
                     IPAFEDAILKIKRYLEDEENLSKASQKILKSRFKQESQL"
     gene            complement(5085..6407)
                     /locus_tag="PLW08_04575"
     CDS             complement(5085..6407)
                     /locus_tag="PLW08_04575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPL51911.1"
                     /translation="METQAFQKIYTKLNQITKATCTVQATGVGNEELATVAGRLAQVV
                     KIIGDNVTLQIFAGTEGIGTDAEVVFFGKAPTLKVSDELGGRFFNAYGEPIDGGPEIE
                     GKEVEIGGPSVNPVRRKQPSELITTGIAGIDLNNTLVTGQKIPFFADPDQPYNEVMAM
                     VALRAQSDKIILGGMGLSNDDYLLYKNTFENAGVIDRIVSFVNTTEDPTVERLLVPDM
                     ALTAAEYFALEKKEKVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKLY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLKRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKLENGFDLSDYDIRVLDFAKE
                     YSDKILAVDVNISTDEMLDRTWELFQKYFNRAEIGIKDEFMNLYWKKG"
     gene            complement(6407..8161)
                     /locus_tag="PLW08_04580"
     CDS             complement(6407..8161)
                     /locus_tag="PLW08_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPL51912.1"
                     /translation="MTTGIVKGIISNLVQVEVSGPVSQNEICYINLGKEKLMAEVIKV
                     VGNIANTQVFESTRGLKPGDTVEFTDHLLEVKLGPGMLSKNFDGLQGDLNKRKGVFLT
                     RGEYTDPLDENSLWKFTPLVKVGDKVSAGDWLGSVPESWITHKIMVPFVMEDEYTVKS
                     IAGEGDYKITETIAVLIDKEGKDIEINMIQKWPVKIPITAYVEKPRPFKLLETGVRTM
                     DTLNPMVEGGTGFIPGPFGAGKTVLQHSISKNAEADLIIMAACGERANEVVELFVEFP
                     ELDDPRTGRKLMERTIIVCNTSNMPVAAREASVYTAMTIAEYYRNMGLKVLLLADSTS
                     RWAQALREMSNRMEELPGPDAYPMDLPAIISNFYARAGFVYLKSGTTGSITFIGTVSP
                     AGGNLKEPVTESTKKAARCFYALSQARADSKRYPAVDPIDSYSKYLEYPEVIEYLNSN
                     VNPHWVKDVNHTKDILLRGKEAQEQINILGDDGVPLSYHDKYWKSELVDRIILQQDGF
                     DKVDQSTPMKRQQYMLELILEICDTDFVFEGFEEVMPYYMQLINICKQMNYMEFQTPE
                     FNNYEAELHKIVAERRAK"
     gene            complement(8175..9062)
                     /locus_tag="PLW08_04585"
     CDS             complement(8175..9062)
                     /locus_tag="PLW08_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPL51913.1"
                     /translation="MKQEYYYFVSGLPNINIDDTKLPWTPEEFLAEAKTQVSENDYHL
                     LEILHLPMDLDNLLRVLYKTNKDRNMEGLYDDAFWEEYLAFLRDVIDNPEVKTPIEFS
                     ALPNFIKEIVLNTLKQEEMQPLPQTELALLKKFYAWTAKSPNDFIRDWFAFDAHIRNI
                     LTAINGRKFNLPYAQYLVGEGETTEKLSKSHTADFGLGKEDELYNALTRIYEQNNILY
                     RERGYDILRWRWIDDYNFFNYFNIDRILGYYCQLRILTRWIKSSPELGKEVFNSILSD
                     LNNSFSFPADFNIKSTQRK"
     gene            complement(9066..9674)
                     /locus_tag="PLW08_04590"
     CDS             complement(9066..9674)
                     /locus_tag="PLW08_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPL51914.1"
                     /translation="MNDQLQDLLKRVYEEGVAKANAEAEKIISQAKEQAEETIAKAKA
                     EAEAILKDAEKKAEDLKKNTQSDLNMAYNHTLTALKQKITDLVLSEALNTKLNDAFND
                     TEFVKKLILETLTAWRENTSSGTIIISEKMKPHLDEFYIKSLKDIFDGKLKVDFSPVM
                     KQGFIIAPEDGTYKIKFEGDDFANLFKNYLRPRTKEILFGNQ"
     gene            9983..10870
                     /locus_tag="PLW08_04595"
     CDS             9983..10870
                     /locus_tag="PLW08_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL51915.1"
                     /translation="MPNKQIQNKIEYLGFRIALAGLKLLPAKAAKSVLKGLFWFGGWV
                     IGIRKYVALKQLSKVYPDKSKKEKAEIVKKLYRNMALTVYESYLMDDDTLYKNIRIQG
                     REYIDNALSLNRGVIFAAAHYGNWEAARILPKAGIPISGIAKPQRNTLFDNYTNAIRE
                     RCGMRIINMKRGLRDIIHEFKENRIVAILIDQNAGSSGLVMDFLGFPASHWKGVAKLS
                     LRYKIPIVPSFARRAEDDRIQFEFFPPILHEELEDTEENYKIVLEEVDKILENQIHKH
                     PEQWFWVHLRWKHSYNMLA"
     gene            10914..>15298
                     /locus_tag="PLW08_04600"
     CDS             10914..>15298
                     /locus_tag="PLW08_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPL51916.1"
                     /translation="MFIDHRYEVLERLGSGTWANVFKVRDIRTDKLFTLKLFQYVSSE
                     ELYSRFSAEDMHRITQLEHPNLNRVVDFGHIADHIYFISEHFDGKPLSRFKFSKSRIN
                     LIYDIAVQISYALNALHTQNILHKDLKLENVLYRLEGNSIVVKLIDYGFSKIDTPKDS
                     QAVSGSLPYLAPEVYLGKSPSKASDFYALGVILYKLTTGSFPFSVDQINALITGGNQY
                     FIPNFPSELNKDIPLPLEKFILRLLDRNPDNRFTNSEEMVDYINRIQPVQYPFSVSWS
                     LINTLRFNSYLVREKYAHQLLDYIPAVENGNGKIISVMGGDGLGKDSIMSLFRYHLLS
                     GKYFIFDYTCSKTDHEAFFALIKEFVQSLSADEIQEYTDLQMISEKFRRYLFESEQEA
                     KTISQTTSELKLDFESVKSLLIELSSNKSIIFIIRNFQYVHHNTIDFLNFMSSYIVKH
                     RILIAITCNEYNKINQIEHTVMLHIPNLNLEETKKYINRLLANITPVSNQNGVPNAFI
                     EEIYKRSAGNPQFIVEILIDLTQRKKLYYNKELHYPLNLSDYQLPTRLLHSIYSRLSH
                     LRDVNYLHLQKLSIVQTPLTRELMLYILKIKDAELYSLLNDSIYNEILRKHRKYYYFT
                     FIEAKQRLFGECNERRQILVSQRVLKFYANETILDFETCKGLIANAFLANDYSCARFY
                     YLKYYEMLNQENEQEQAFEAILNVLNLDFNPALQVPLQNIIADIAIFQEKMEMTGFFE
                     RAGFILQRISELPDFFEKHYLLGTVDFLAENLRQALKYFQTAEKYVHTGKHQLLVWLA
                     YAQIYTKLDPSKMKHYIDKALKQEMTLELQIAFVDRLAVYYSLNKDFDAAIKTIESFL
                     EKLPTENNTKVMIRLAAMHNDLGVFYSAQKKIEEADEHLVTALNIWKRYNIKRYLGLI
                     YNNLCDLYLKQGLTVIAEHYSELAYQYSDDLNLTMSKALALLNQGEAKIKMGDFSTAE
                     QKLLKCQELVLSKGSDNYLQSVKRNLALAKSKIKGFGHYYTFIWENEPELLNGQIKEM
                     SPLVKTYFYYLYETGNAKKLRRLLTKNVHINYKHIHEEEFYHNALALTAMVEKDYERA
                     LNELKIALRHAGEINNNYAIAVFYVLQIRCYYGLKDVELARELIEIALPVIKNNQYRY
                     WQCELEVLALKLDLLNPDIPLRVILRQVNEYLIAWQEYEYYQLNVELYQIKIQIFTEL
                     KQEELAQAEFENYKAYLEKITENISPDDRQNYFTINLYFLKNLKNFNLLPVVSRSRDL
                     VKQWNDMLYNITNIHNIERIKFLIEKGLIQILAPWQFWLMQYSEKVSSYTCFQSYNTG
                     KDNLINTQLMPFIEKAYKTDNLVLLEQNNRHLMIVPFQIGSKKIGFLLVSDKGEMPFT
                     KQEIYFMRSTKQHLSALLVRIEDFSQITQRIEKMNQLMAITHELMRIVNLNELEHAIV
                     SACIDFTNSTRGFLIKRDSDGAIIY"
     assembly_gap    15299..15398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15399..16552
                     /locus_tag="PLW08_04605"
     CDS             <15399..16552
                     /locus_tag="PLW08_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPL51917.1"
                     /translation="STRGFLIKRDSDGAIIYKVQLNDAKQILPTVSGVSKTVLSLSQN
                     TQEIVSTYNAVEDNRFKSAISVQDYVLHSIFCAPLVVDNNVYGYIYLDNLNDNSREMY
                     LNTELIKLLLEQITIALKNAMLYENLLQKNNELNTFETLKDEFMAIVSHELNTPLTTL
                     QGYVTRLKRNLFADEEEKRDIINKIETSVKKLILTINDITTLNTYNLTKSLPLTDISV
                     QEILKIIHQEVQILSRNRKMFIKLEIEKDLPPVKANWEALHLMIYNLVLNAIRFTNDF
                     GTVIIGARKSAFQSEKIDGKEALVLYVQDNGIGIPEYQLQNVFRTFYELNEIYAHKSG
                     IIEYRSSGLGLGLSTSKRIAELHGGKIWIKSKENEGTTVFVSIPLKQNK"
     gene            16569..19499
                     /locus_tag="PLW08_04610"
     CDS             16569..19499
                     /locus_tag="PLW08_04610"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL51918.1"
                     /translation="MKYFLFTLLALTLTCFCFAEFIVPQNNAPLMAATNSENYAYRYI
                     VELYNVGDTLNLTKEITQFKATYPESVYLPYIKFIEGNLAMEANAFSQAIDIYNSLLE
                     TNLSQNVRSEIILNYAYCLTQLKQYDSALKLLQHLETETGNPTYLELANSLRGDIYFQ
                     LGQYYSAEKAYLRAVKAFPDNEALHFSLFSTFLALKKDDQAFTLLKNQSPKDTYFINY
                     IQSWLEYLLENEYYNDFDNFVESYNLDDIAFVPEIVDIRIRRALRTSDFDQISRLLEG
                     LNNNTPQFNYYRALVLIRDGKENQADSLLAELVNSTQPEIAVPAYLERLKLLFKKNPQ
                     NAIEQLQTYLKQTPNELMKAELYYTLGYFYFQQKNYTEAIRQLGQARNYETSRELNSR
                     IDFLIAEAFYFAGNSNLAKDAFNRYLSRYPSGNKADKAYFYLGYLSFLEKDYTEAKNN
                     FQELINLYPESFYGNEALYYLAEMDFYLANYNLALKKYLYLYEKNPENEVIALRIAQI
                     YFYLGDYDQSENFLQNLVPNYDICLLKGNIMLAKKNYSPALEQFILAEGFATDNVRKI
                     EAQSYRALCLYQMKRFKDASTLYLKLSREKESPDTYLFLAAKSAYAAKDYHLALELYN
                     NFIDKYPESSHFLEALTDIANTYYNMGNYERAVNDWINILTRFRNTTKFTENQLATIH
                     DALVGLELALKRVNNKDELLNDLLVLPDTFFSEYIKFELNYILMKVYADELKWADLIA
                     TAEKIRTEFPEQKHEDIELLMATALIELNQYAEADTLLAELYAQSKNNEALLKWAELE
                     YITGNYDSALEKYKLAYKNNPDGATWVKLLECSEANNYKDFDKLWSLGSNYLKNHPEV
                     NVIRMRQLYNAQRFEEAEALIEFIINNSLSTLDHCNAFLVMGMIDYHRHSYLSAISTL
                     KKVILLFPEYKEIRSKAIYYSVLSLLESGAKTEAEALFFKYREELDEAAKMELTDVFL
                     EVNQ"
     gene            19496..20974
                     /locus_tag="PLW08_04615"
     CDS             19496..20974
                     /locus_tag="PLW08_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51919.1"
                     /translation="MKRTLLILTLALTLTLLFSQTQELPDLTVSGESSFKPFLYKRSL
                     LFSPELNMGDSLPAFVPPGVPLQEKPKPIPAMQHRSYLQFEGNMDFGLNSFISYYPDS
                     FILNALTYTLDMRSPISEMLSVHNNLFLGTELSSHFPLSFRFQNVSSQADDFDNTVLD
                     FNFSHHRPDLTIGNVSFKDISAQLGYNYIYQKNLNKPYKCNYLNVYLASCLEEEILNW
                     KTKFMVKSGDFGIQIAPVYNGDIMDISKPGIHLLADAYTIVPSLEFLYRYPITGWGVL
                     SLSNVPLLEGNDYLSFLEATPWISFSDAHKLKKTPLNFNAGLEYLYPQKINFSLGRLN
                     INNNLRYEIDSPVLVSTNNYRIPTLRYTDVFSNLTSVEAFFKMDKLNMHQGLELELAY
                     LTESDYTRAPYRPLLNLDSRFAYGYGNWYFNLDILQHYFTKDHRGHDLPEAIILNLGA
                     EYHKDNSAAYAQLANLFNRKEWVFSEQPGKGRNLYIGLKHRF"
BASE COUNT         6540 a         4110 c         3743 g         6575 t
ORIGIN      
        1 cagtgaaacc aatagtcata aggtcttgcc agttgagtag ttaaataatc attttcttta
       61 gcacggcgtc ctataaggat ttgaaactca tcatttatct tcaatttcag gattttatcg
      121 gcaagaatag cgttttggac aattttgtgt ccggttggtt tggagggcaa aattccgcta
      181 tccggtaatt cacctttttc cgctttggca atgaggtctt ttattttttc aatttcggca
      241 tctgtctgag ccagattttt ggtaattatc tgtaagccat ttttggcttt atgatacctt
      301 ttcagatagc tttgcaaatt ttcctgcggg cttttgtctt tctgtaaaag tatttctatt
      361 tccttcaaat cggggtcaag ataattgatt gccgtaaaac tttcccttcc ggttttaaga
      421 ttatgcagat tggttttcaa acattctgca caagcgagcc aaaaatccgc tttttccgca
      481 tttgttaggt cttgttgtaa tatttctctt tttttctgta atttttgcag ttcttttttt
      541 agggcagtgc atattttttg actattttct gcctcttctt cttgttttaa cttattgtaa
      601 tacagattga taaaataatc gttgcaggtt gcgctaccat caggataagg ggattttact
      661 tcttccaaaa tcggttgaaa agatgttttg ggagcagtgt aaacaagtcc cggcaaaatt
      721 tgtctttgag gattatccgc atagctgtat ttatgaaggg catcaagaat atgcaattcg
      781 gaatcagtta aaatggcatt gggttgagga gtcatcaatt ctgcgataag ataatatttt
      841 ttattctgct ggtagatatc tctttgctga agctctaaat aggcaagacg gtcattttca
      901 gctaaagaaa tacctgtaag atgacaattg ttaagctttt gccagatttt tttcccttcc
      961 tgaggtagct ggaaattatc ctgcagaaaa atgaaggcat cgcgtttact aagcactatg
     1021 cacagggctt ttttatcttt tgtctgcaat actaaaagct ctggaaacaa taaaacactt
     1081 tctatcactg cttgataacc aatcatttct tgtgtccatt gcttaagata agaatatttc
     1141 actttagtta ttttcctttt gtcttgacaa aaaaacaggt tatatttttg aatgccaaag
     1201 tataatatat tacaaaggag tcaagcatga aagctacaac agcgattata gtagcagttc
     1261 tggcaattct aactatcgtg tttgctgtcc tttggattaa tgccaacggc aaatccaaag
     1321 atattatgaa aagcaacgaa gaattgaaaa aactgtatga gacatctact tccacgatca
     1381 acgaaattca atcaagcttg gaatcgctgg aacaagacct ttcgggacaa ctttttacgc
     1441 agaaagaaat tcccggaaca tcccctgaag agcggagaaa tcgcattatt tcttccatag
     1501 ctaatatgcg agaccaaatt gaagcggata aaaagaaaat tgctactctg gaaaaacaac
     1561 ttgccacttc caaaagccaa ttgaaaggtg tgcaggaaat tgtgaataaa cttaaagcca
     1621 gtatcagcga aaaagaacaa attatggatg aattacaaca gcgcttgggc attttgaacg
     1681 aaaccctgga aacggaaaga agaaccagtg ccgaagaaat tcagaaacgc gagatgacga
     1741 ttacagaaaa agaacaacag attactcaac aaagcattga agtaaatact atttattatg
     1801 tatatggtac ccgcaaggaa ttaatgtcta agggaattat ggatagaaaa ggcggacttt
     1861 tgggtattgg aaaggtttct accgtaaaac aaaatattcc tattgagcaa tttacctcta
     1921 tgaacctgct ggaaacacag caaatcagtt ttccggcaac gaaaaagggc tattctatcc
     1981 ttactaatca ccctgctact tcctataaag tggaaaaaga aggcgaccag aatatcctta
     2041 ctattcttga cgcaacaagc ttccgtaaac aaaaattcgt agtaatagaa ctgctgtaac
     2101 ctatataaat tttaaccctt tcaaaaaggt gaggttaaaa aaaacctcac cttttttgtt
     2161 ttgcaattta gtttttaagc taaagttgga attactccac caattaggaa actggcagcg
     2221 aaagcaataa ttgcataaag ctcaggaaac acacccaaaa taagggtttt ggcaaaaaca
     2281 tcgtgtcctg aaccgatact ttcaatacct gtggcgacaa ttcttgactg gtgataggca
     2341 gaaataagtt ccacagttcc tgaaatcaaa cctgctccta aaatggcaca ggcctgaagc
     2401 atattaattt ggggagtaat ataagctttc aggatgaaga aagaaccaaa accatataga
     2461 ccctgcgttc caggtaaagc ggaaaggatc atacagctgg gaaaaatatc atcccttttt
     2521 ttcattgcac caactgatgc acgaccaccg ataacagttc cccaggcact tcccattccg
     2581 gaaagagcta ccatcaggaa aagtccgatc caagccaaaa aatatgccaa attttggctc
     2641 gccatagtgg caacaattgg ttgagctatt gtgtttaagg aatcaggcat tttaacctcc
     2701 cttgttattc ctttttccca aagggtttat attccagccc gggacctgca aaagcagcat
     2761 ttttgtaaaa ctccacgaaa gtaagtcgta aaggatgaac aaaaccgcta agacctccca
     2821 aggcaatatt taaagtatgt ccaaaaagca taaacagcac aaaaataata ggaccgacaa
     2881 tcggaattcc attagcaatt tgacttccaa tagagttcat tacaaaccct aaaattgcgc
     2941 tggaaacgct taaagcaaaa agacggatgt aagataaaat atcaccaaaa taacctgtaa
     3001 caatttgata aagtaaccaa aatccgttga gaatattgag aataatattc ttacctggac
     3061 tattgaacag caaaatcata gccaatccga tataaatagg gtatttaata tagggctgaa
     3121 ctttgctgat atttgctcct aaaagcgaag aaccgagaac cgttaaccca aacaataata
     3181 accaggtacc aatagtactt agcccaaatt tgaaaccgtt ttttttcatt ttcttcactg
     3241 ctgcaatggc tatcccgaaa aggatttgga tagcacctaa aagcaaagca tagttgaaga
     3301 tttgattgtt atccttaatt aatattttat cctgaatagc cggaatcgtt ttcaaatcga
     3361 aagctaaagc actacccatc acccaaccca ttattatgga agcagttcca aagattatta
     3421 acagggtaag aatgtctctc ataccctggt ttttcatctt caattttaag ataacagcca
     3481 aaatgataat aactatacca taaccgatat ccgcattgca aaaaccgaaa aacaaagcaa
     3541 agaaaggtgc aaagaaagga gttaaatcaa attcatcgta ataaggtaac atatacattt
     3601 tgctaatggg ctcaaaaaga cgggcaaacc agttatttct taattttatg gggggatttt
     3661 cttcaggttt ggcatcttct gaaacataaa taatgttatt cttctccaga aagtccttta
     3721 aaccctgttc caaactgaca ggaatccaac cgcttaaaag acggatagta ttttccgctt
     3781 catatttacc ttgctggaga gcatcttcaa attcgtattc ccgacttagg cgtttaagct
     3841 catcctgaaa catttcaata gcagtaggag ctatggaata caagtaatca tcaatatctt
     3901 tttgctcatt ttccagttcc ttcagcttgt cttcaaattc ctgaagggta tgctgatgaa
     3961 aagaaaatgt atccgcttct aaagccggat ctccattatc tgtaataact acaaagtata
     4021 gaattccgcc aacttcgtta ataattttta agggatattt ttcctgccat tccggtttaa
     4081 agtgattttt taagcaggta tagaaacgaa tggtcaatcc gtattcttta agtttgcgca
     4141 ccaaatcgta atcaaaatgc ccccagggac ttaaatcccg aatttgtttt ttaactgtat
     4201 ctatagagtg ttgaagttta tccttttctt ctcttgcttc atttatttta ttcagcaatg
     4261 cctttgtggg taaaggactt gcctgcttag gtgcattgga ttttagtttt gtaaggaatt
     4321 taacacattc accatattcc ttcatcagtt caaggttttt ggtttgggat tcggtttttt
     4381 catcacagct acgaataata tgcaccacac ccagcttttg gagctctgcc aaaaagttct
     4441 cgtaatcctg atgatagaga acgaaggtat atttacgcat cttttctatc atagctggct
     4501 ctcctgttta aaacgggatt taagtatttt ctgtgatgct ttggataaat tttcttcatc
     4561 ttccagatag cgttttattt ttagaatggc atcttcaaag gcaggtattt gtactttttc
     4621 gtagagattc actttctgtg ttgtcttttt ccgcacttgc tccaaaattt gcatcttgcg
     4681 aatataaaag tccctttcaa tttggagttg gcaaatttct ttcagcaaag caattccttc
     4741 gggataccag gaaggagctt taaaaaggtt atagtccgct atttcaaatt ctatgttttc
     4801 caataggggg gtttttacac cagctatttt acgggtttta atttccacat ttttaatttt
     4861 cacgatgtca ggatcaaatt ccacaaacaa tttcataaaa gcatcaagtt ctgcagtacg
     4921 ttctttcacc ttttggtcta aagcaaatgc ctggtcgcgt gcttttttaa cttccagacg
     4981 taaggcactt tccttgtttt tcaaggttgg caaagctctt tgcctaaccg caagttgttt
     5041 aatcagttgt aactgagaga ttttattata ttgaaatttc aggttcatcc tttcttccaa
     5101 tacaaattca taaattcgtc tttgatacct atttcagcac ggttgaaata cttctggaaa
     5161 agttcccagg ttctatccaa catttcatcg gtgctgatat taacatcaac agccaaaatt
     5221 ttatcggaat actctttggc aaaatcaagc acgcggatat cataatcgga taagtcaaaa
     5281 ccgttttcca gcttggtttt agcattggca gcatcggcat aaagacgcac tgcagtattc
     5341 attacctggg gatggtcttc ccttgtcttt ttgcctatca caagctgttt caaacgagaa
     5401 agagaacgga aaggatcaac aataactttt ccaatatccg tatcgcgttt caggaaaagc
     5461 tgtccttctg ttatgtaacc tgtattatcg ggaatggcat gagtaatatc tcctcccgaa
     5521 agagttgtaa ctgcaataat tgttatggag ccaccatcgg gaaattgcac tgctttttca
     5581 tagagctttg ctaaatcact gtaaagcgaa ccgggcatac tatccttgga aggaatttgg
     5641 tccatacggt tggaaacaat acttaaagca tcgcagtaaa gtgtcatatc agttaacaac
     5701 accaagacct tttctttttt ttccaaagca aaatattctg cagccgtaag cgccatatca
     5761 ggaaccagaa gtcgttccac agtagggtct tccgtagtat ttacaaaaga aacgattcta
     5821 tctatcactc cagcattttc aaaagtgttt ttataaagca gatagtcgtc gttggaaagc
     5881 cccatacccc ctaaaataat tttatcactc tgagctctta aggcaaccat agccattact
     5941 tcattatagg gttggtcagg gtctgcaaag aaaggtattt tttgtcctgt aaccaaagta
     6001 ttattcaaat caattcctgc aattcctgta gtaatcaatt cggagggttg ttttctgcgc
     6061 acaggattaa cagaaggacc tccaatttcc acttctttac cttcaatttc aggacctccg
     6121 tcaatcggtt caccataagc attgaaaaac ctgccaccta attcatcact aactttcaaa
     6181 gtaggtgctt ttccgaaaaa gacaacttca gcatcagttc caattccttc agtcccggca
     6241 aaaatctgta gagttacatt atcacctata attttaacta cctgagccaa acgtcctgca
     6301 actgtagcaa gttcttcatt tcccactcct gttgcctgaa cggtgcaagt agctttagtt
     6361 atttgattga gtttggtata tattttttga aaagcttggg tttccattat ttagctctcc
     6421 tttctgcaac tattttatgt agttccgctt cgtaattgtt aaattctggc gtttgaaatt
     6481 ccatataatt catctgctta cagatattga ttaactgcat ataatagggc ataacttctt
     6541 caaagccctc aaacacgaaa tccgtatcgc aaatttccaa aattagttcc agcatatatt
     6601 gctgtctttt catcggagtg gattggtcta ctttatcaaa gccatcctgt tgtaaaataa
     6661 ttctatccac cagttcactt ttccagtatt tgtcatggta cgaaagcgga actccgtcat
     6721 cacctaaaat atttatttgt tcctgtgctt cttttccgcg caagagaata tctttggtat
     6781 gatttacatc cttcacccaa tgcggattta cattggaatt caaatattca atcacttctg
     6841 gatattccag atatttactg taggaatcaa taggatcaac tgcaggatag cgtttactgt
     6901 ctgctctggc ttgagaaagt gcgtaaaaac aacgtgctgc tttcttggtg gattccgtaa
     6961 ccggctcttt caaatttcca cccgcaggag aaacagttcc tataaaagtt atagaaccag
     7021 tagttccact ttttaagtaa acaaacccag cacgggcata aaaattggaa ataatggctg
     7081 gaaggtccat tggataagca tccggaccgg gaagttcttc cattctgtta ctcatttctc
     7141 ttaatgcctg agcccaacgc gaagtggaat cagccagcag taaaaccttg agccccatat
     7201 tgcgataata ttctgcaatt gtcattgcag tataaacaga tgcttctctt gctgctacag
     7261 gcatattgga agtgttacaa acaatgatag ttctttccat aagttttctt cccgttctgg
     7321 gatcatccag ttcgggaaat tccacaaaga gttccacaac ttcatttgct ctttcaccgc
     7381 aggcagccat aataattaag tccgcttccg catttttgga tatggaatgt tgtaaaactg
     7441 tttttcctgc tccgaaagga cctggaataa agccggttcc accttccacc ataggattta
     7501 gtgtatccat tgtgcggaca ccagtttcca aaagtttaaa gggacgcggt ttttctacat
     7561 aagcagtaat aggaattttc acaggccact tttgaatcat attaatttct atatccttgc
     7621 cttctttgtc tatcaaaacg gcaatggttt cggttatttt atagtcacct tccccagcga
     7681 tgcttttaac agtatattca tcttccatca caaaaggaac cataatttta tgagtaatcc
     7741 aactttcggg aacggaacct aaccagtcac ctgcacttac tttatctcca actttgacaa
     7801 ggggagtgaa tttccagagg gaattttcat ctaaggggtc agtatattca ccccgagtta
     7861 aaaaaacacc ttttctttta ttgaggtcac cttgcaagcc gtcaaaattc ttggaaagca
     7921 ttccgggacc aagttttact tccagcaaat gatcggtaaa ttcaacagta tcacctggtt
     7981 ttaaaccgcg tgtgctttca aaaacttgag tattggctat gtttcctaca actttaataa
     8041 cttctgccat caacttttct ttgcccagat tgatgtagca gatttcattt tgggaaacag
     8101 gacctgaaac ctcaacctga acgaggttgg aaattattcc tttaactata cctgttgtca
     8161 tatttgttta ccttctattt tctttgcgtg ctttttatat tgaaatcagc gggaaagctg
     8221 aaactattgt tcaaatcgct caaaatggaa ttaaaaacct cttttcccaa ttcgggagat
     8281 gactttatcc agcgagttaa aatacgcaac tgacaatagt aacctaaaat gcggtctata
     8341 ttgaaataat tgaagaagtt ataatcatca atccaccgcc agcgtaaaat atcataaccc
     8401 ctttcccgat agagaatatt gttttgttcg taaatgcgag ttagtgcatt gtaaagttca
     8461 tcttctttgc ccaaaccgaa atccgcagta tggcttttac tcagtttttc cgttgtttca
     8521 ccttctccga caagatattg ggcataaggt agattaaatt tgcgtccgtt tatagcggtt
     8581 aaaatatttc ttatatgagc atcaaaggca aaccagtccc ggataaaatc attcggactt
     8641 tttgctgtcc aggcataaaa tttctttaga agtgccaatt cagtttgtgg tagaggttgc
     8701 atttcttcct gttttaaggt atttaacact atttctttga taaaattcgg gagagctgaa
     8761 aattcaatag gagttttaac ttccggatta tctattacat cgcgtaaaaa agccagatat
     8821 tcttcccaga aagcatcatc ataaagacct tccatattgc ggtctttatt tgttttatac
     8881 aaaactctca ggagattgtc caaatccatc ggcagatgta aaatctccag aagatgataa
     8941 tcattttcgc taacctgggt ctttgcctct gccaaaaatt cttccggagt ccaaggcagt
     9001 tttgtatcat caatattgat gttgggcaat ccagaaacga aatagtaata ttcttgtttc
     9061 ataaattatt gattaccaaa gaggatttcc ttagtacgag ggcgtaagta atttttgaac
     9121 aggttggcaa aatcatctcc ctcaaattta attttataag ttccatcttc gggagcgata
     9181 ataaagccct gtttcataac gggtgaaaaa tcaactttca acttaccatc aaagatatct
     9241 tttaaggatt taatataaaa ctcgtctaaa tggggtttca tcttttcact gatgataatt
     9301 gttccggatg aagtattttc cctccaagct gtaagggttt ccagaataag ctttttcaca
     9361 aattccgtat cattgaaggc atcgtttaat ttagtattga gagcttcgga aagaacaagg
     9421 tcggtaattt tttgcttgag agctgtgaga gtatgattat atgccatatt caaatcggat
     9481 tgagtattct ttttcaggtc ttctgccttt ttttccgcat ctttcaaaat tgcttctgcc
     9541 tccgctttgg ctttggctat ggtttcttcc gcttgttcct ttgcctgact gattattttc
     9601 tcagcttccg cattggcttt ggcaacacct tcttcataaa cccgttttag taaatcttgc
     9661 aactggtcgt tcataatgta tctccagtgt tatcactttt tgtgacattt aaagaatgcc
     9721 aaatttttgt caattaaaat cggttacctg gattttttat tcataacacc gaaaacactg
     9781 aaagcaatta gaacaccgaa atatttttta gcaaagagaa aatagcaaag gccctctcaa
     9841 ccttaccaac cctttacttc ctaattcttt tctttctctc tttgtaaaaa tccacctccg
     9901 ctttcagata tttcttgtca gttcaagccc gttaaaaata atggtaacag gagaaaaagg
     9961 tggatttatt aaggatagcc aagtgcctaa taagcagata caaaataaaa tagaatactt
    10021 aggtttcagg atagctcttg cggggctaaa acttcttcca gcgaaagcgg caaagtcagt
    10081 gctcaaaggt ctcttctggt ttggtggttg ggtgattggc atccgtaaat atgttgcctt
    10141 aaaacagctt agcaaggttt atccggataa aagcaaaaag gaaaaggcag aaattgttaa
    10201 aaaattatat cggaatatgg cgttaaccgt ttatgaaagt tatctgatgg atgacgacac
    10261 gctatataaa aacatccgaa ttcaaggcag agaatatatt gacaatgcat tatctctaaa
    10321 taggggtgtg atctttgccg cagcgcatta tggaaactgg gaagcagcaa gaattttgcc
    10381 taaagcagga attcccatca gcggaatagc caaaccccaa cgcaatactc tatttgataa
    10441 ttacaccaat gccatccgcg agcgttgcgg aatgcgaatt atcaatatga aacgcggttt
    10501 gcgagacatt attcacgaat ttaaggaaaa taggatagtt gcgattctta ttgaccaaaa
    10561 tgcgggtagt tcaggattgg taatggattt tttaggtttt cctgcttcac attggaaagg
    10621 agtagccaaa ctttctttgc gttacaagat tcctatagtt cccagttttg cacgcagagc
    10681 ggaagatgac agaattcaat ttgagttctt tcctccgatt ttgcatgaag aactggaaga
    10741 tacggaagag aattataaaa ttgttctgga agaggtggat aaaatattgg aaaatcaaat
    10801 tcacaaacat ccggaacagt ggttttgggt gcacttgaga tggaaacatt cctacaatat
    10861 gcttgcctga aaatacatcg taaaaccaat aaatcaggca atggagcttt aatatgttta
    10921 tagaccatcg ttatgaagtt ttagaacgct tgggttcagg aacttgggct aatgtgttta
    10981 aagttcgtga tattcgtact gacaagcttt ttacgctaaa gctgtttcag tatgtttctt
    11041 ccgaggaatt atattctcgt ttcagtgcgg aagatatgca tcgcattact caacttgagc
    11101 atcccaattt gaatcgggta gtggattttg gtcatatagc tgatcatatt tattttatca
    11161 gtgaacactt tgacggcaaa ccactttctc gtttcaaatt cagcaaatcc aggataaatc
    11221 tgatttacga tatagccgtt caaatttctt atgctttgaa tgcacttcat acccagaaca
    11281 ttttacataa agacctcaaa ttggaaaatg ttctttaccg tctggaaggc aattccattg
    11341 ttgtaaaact gattgactat ggtttctcta aaattgatac tcctaaagat tcacaagccg
    11401 tttccggttc attgccttat ctggcgccgg aagtttactt gggaaagtcc cccagcaaag
    11461 cgagtgattt ctatgcgttg ggagttattt tatacaaact taccacaggc agttttcctt
    11521 tcagtgtaga ccaaataaat gcgttaatta ccggtggtaa tcaatatttc attcccaatt
    11581 ttcccagcga gctgaacaag gatattccct tacctctgga aaagtttatt ttgcggttac
    11641 ttgaccgaaa tcccgataac cgttttacaa acagtgaaga aatggtagat tacataaacc
    11701 gcattcaacc ggtgcaatat cctttttccg tctcctggtc tctaatcaat actttgcgtt
    11761 tcaatagtta tctggtgcgg gaaaaatatg cacatcaact gttggattat attccagcag
    11821 tagaaaatgg caacggtaaa attatatccg ttatgggtgg agatggtttg gggaaggaca
    11881 gtattatgtc ccttttccgt tatcatttgc tcagcgggaa atactttatt tttgattaca
    11941 cctgctccaa aacagaccac gaggcatttt ttgctctcat caaagaattc gttcaatctc
    12001 taagtgccga tgaaattcaa gaatatacag atttacaaat gatttccgag aaattccgtc
    12061 gctacctatt tgaatcggaa caggaagcaa aaacaatcag ccaaaccact agtgaattaa
    12121 aattagattt tgaatccgtt aaaagtttac tgattgaact ttccagcaac aaatccatca
    12181 tctttattat tcgcaatttc caatatgtgc atcataatac tatagatttc cttaatttta
    12241 tgtcctctta catcgttaaa catcgcattt taattgccat tacttgtaac gaatacaata
    12301 aaatcaacca gattgaacat actgttatgc tgcacattcc taacttgaat ttggaagaaa
    12361 ccaaaaaata tatcaatcgt ttattggcaa atataactcc cgtgagtaat cagaatggcg
    12421 ttcctaacgc ttttatagag gaaatataca aacgttcagc tggaaatccc caatttatag
    12481 tagaaatttt aattgacctg acccagcgga aaaaactcta ttataataaa gaattgcatt
    12541 atcccttaaa ccttagtgat tatcaattgc caactcggct gttacattct atttattcac
    12601 gcttaagtca cttaagagat gttaactatt tacatttgca gaaattaagc atagttcaaa
    12661 cccccttaac ccgtgaattg atgctttata tcctgaaaat aaaggatgca gaattatatt
    12721 ctttgctcaa cgacagcatt tataacgaaa ttttaagaaa acaccggaaa tattattact
    12781 tcacttttat tgaagcaaaa cagcgacttt tcggcgaatg caacgagaga aggcaaattc
    12841 tcgtttctca gcgtgtttta aaattttacg ctaacgaaac gattttggat tttgaaactt
    12901 gtaagggttt gattgctaat gcttttctgg caaatgatta tagctgtgcc cgtttttact
    12961 atttgaaata ttatgagatg ttgaatcagg aaaatgaaca agaacaagca tttgaagcca
    13021 ttcttaatgt tttgaatctg gattttaatc ccgctctgca agttcctctc caaaatatta
    13081 ttgctgatat cgctattttt caggaaaaaa tggaaatgac cggtttcttt gagcgtgccg
    13141 gtttcatctt gcagagaata tctgaactcc ccgatttctt tgagaaacat taccttttag
    13201 gaacggttga tttcctcgct gaaaacctgc gtcaagcatt aaaatatttc cagacagccg
    13261 aaaaatatgt gcacacggga aaacatcagc tacttgtttg gttggcttat gctcaaattt
    13321 atactaagct tgacccctct aaaatgaaac actatataga taaagcccta aaacaagaga
    13381 tgactttgga attgcaaatt gcttttgttg accgtttggc agtatattat tcgctcaata
    13441 aggactttga tgcagcaata aagaccatag agagctttct ggaaaagctt cccaccgaga
    13501 ataataccaa ggttatgatt cgcttagccg ctatgcataa tgacttaggg gtattctaca
    13561 gtgctcagaa aaagattgaa gaagcggatg aacatctcgt tacagcttta aatatctgga
    13621 aaagatataa tataaaacgc tatttgggtt tgatttacaa taatctttgc gacttatatc
    13681 taaaacaggg attaaccgta attgccgaac attattccga actggcttat caatattccg
    13741 atgacctcaa tttgacaatg tccaaagcat tggctttatt aaatcagggt gaagcaaaaa
    13801 tcaaaatggg cgatttttct actgctgaac agaaactttt aaaatgccag gaattagtcc
    13861 tctccaaagg tagtgacaat tatctccagt cagtaaaacg caatcttgcc ttagctaaaa
    13921 gcaagattaa gggttttggg cattattaca cctttatttg ggaaaatgaa ccggaactgc
    13981 ttaatggcca gattaaggaa atgagtcctc tggtgaagac ctatttttac tatctttacg
    14041 aaacaggtaa tgctaaaaaa ctgagaagat tactaaccaa aaatgttcac atcaattaca
    14101 agcatatcca cgaagaagaa ttctatcata acgctctggc tttaacagct atggtggaaa
    14161 aggactatga aagagctttg aatgaactga aaattgctct gcgtcatgca ggagaaataa
    14221 ataataacta tgctatcgcg gttttctatg ttttacaaat ccgttgttat tacggcttaa
    14281 aggatgtaga acttgcgcgc gagttaattg aaattgctct gcctgtcatc aaaaataatc
    14341 aatatcgcta ttggcaatgc gaactggaag ttttggcttt aaagctggac cttttgaatc
    14401 ccgatattcc cctcagagta attttaaggc aagtaaatga ataccttatt gcctggcagg
    14461 aatatgaata ttatcagctg aatgtggaac tctaccaaat taaaatacag atatttacgg
    14521 aattaaaaca ggaggaattg gcacaagcag aatttgaaaa ttacaaagca tatttggaaa
    14581 aaatcactga aaacatcagt cctgatgaca gacagaacta ctttaccata aacctctatt
    14641 tcttgaaaaa tctgaaaaac tttaaccttt tacctgtcgt ttcccgcagt cgggatttag
    14701 ttaaacaatg gaatgatatg ctttacaaca ttaccaatat ccataatata gagagaatca
    14761 aattcttaat tgaaaaaggg cttattcaga ttttggcacc ctggcaattc tggctgatgc
    14821 aatattccga aaaggtttca tcgtacacct gttttcaaag ttacaatacg ggaaaagaca
    14881 atttaatcaa cacccagctt atgcctttca ttgaaaaagc atataaaacc gataacctgg
    14941 tcttactgga acaaaataat cgtcatctga tgattgttcc ctttcagatt ggctctaaaa
    15001 aaataggttt cctgctcgtt tccgataaag gtgaaatgcc ttttactaaa caggaaatct
    15061 attttatgcg ttccacaaaa cagcatctct ctgctctgct ggttagaatt gaggatttca
    15121 gtcagattac tcagcgcata gaaaaaatga atcagctgat ggcaattact cacgaattga
    15181 tgagaattgt gaatctgaac gaactggaac acgccatcgt ttctgcctgt attgacttta
    15241 ccaatagcac gaggggtttt ctcatcaaac gcgatagcga cggagcaatt atctataann
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat agcacgaggg gttttctcat
    15421 caaacgcgat agcgacggag caattatcta taaggttcag ctcaatgacg ccaaacaaat
    15481 tttgccaaca gtttctggtg tcagcaaaac agttttaagc ttatcccaaa acacccagga
    15541 gattgtttcc acctacaatg cagtggaaga caaccgtttc aaaagtgcca tcagcgttca
    15601 ggattatgtt ttacacagca ttttttgcgc tcctcttgtt gtagataaca atgtttacgg
    15661 ctatatttat ttggataacc tgaatgataa tagtagggaa atgtatttga acacagagtt
    15721 aattaagctc cttctggaac agattaccat tgctttaaaa aacgctatgc tctatgaaaa
    15781 tctgctccaa aagaacaatg aactgaatac ttttgaaacc ctgaaagatg aatttatggc
    15841 tatcgtttct cacgaactga atactcccct cactactttg cagggttatg taactcgtct
    15901 taaacgcaat ctctttgccg atgaggaaga aaaaagggat atcattaata aaattgaaac
    15961 ttccgtaaaa aaacttatcc tcactatcaa tgatataact actttaaata cttataactt
    16021 aaccaaatca ctgcctttaa ccgatatctc ggttcaggaa attctgaaaa tcattcacca
    16081 ggaagtccaa atcctttccc gaaaccgtaa aatgttcatc aaattagaaa tagagaagga
    16141 cttacccccc gtaaaagcca actgggaagc ccttcatttg atgatttaca accttgtact
    16201 taatgctatc cgttttacca acgattttgg caccgttatt ataggagctc gcaaatccgc
    16261 tttccaaagt gaaaaaatag acggaaaaga agcgttggtt ctatatgttc aggataacgg
    16321 catcggaatt cctgaatatc aactccagaa tgtatttcgc acattctacg aactaaacga
    16381 aatctatgct cacaaatccg gaattataga atatcgcagt agcggacttg gtttgggact
    16441 ttccacctcc aaacgcatcg cagaacttca cggcggcaaa atttggatta aaagtaaaga
    16501 aaatgaagga actaccgtgt ttgtttccat tcccttaaaa caaaataaat aggtgagatt
    16561 aaaagtttat gaaatacttt ctttttactc tgcttgcctt aacccttacc tgcttctgtt
    16621 ttgcagaatt cattgtgccc caaaataatg caccattgat ggctgcaact aatagcgaga
    16681 attatgctta ccgctatatt gtagaactat acaatgtagg tgacactctg aatttgacca
    16741 aagaaattac tcaatttaaa gctacctatc ccgaatccgt atatctacct tacatcaagt
    16801 ttattgaagg caatctggct atggaagcaa atgccttttc ccaagcgatt gatatttata
    16861 attctttgtt ggaaacaaat ctcagccaga atgtacgaag tgaaattatt ctgaattatg
    16921 cttattgtct tactcaacta aagcagtatg attctgcact aaaacttctt cagcatctgg
    16981 aaactgaaac tggaaaccct acttacttgg aactggcaaa cagtttgcga ggggatattt
    17041 actttcagct cggtcaatat tattcagcgg aaaaggcata cctgagagcc gtgaaagcat
    17101 ttcccgataa tgaagctctt cacttttccc tgttttctac tttccttgct ttgaaaaaag
    17161 atgatcaggc atttactttg cttaaaaatc aatcacctaa agatacatac tttataaatt
    17221 acattcaaag ctggttggaa tatcttttag aaaatgaata ttataatgat tttgacaact
    17281 ttgtagagtc ctataatttg gacgatattg ctttcgttcc agaaatagtg gatatcagaa
    17341 tccgtcgcgc attacgcaca tccgattttg accaaatctc ccgtttgctg gagggtttaa
    17401 ataataatac tcctcagttt aattattacc gcgctctggt tttaattagg gatggtaaag
    17461 aaaaccaggc agattcgctt ttggctgaat tagtaaattc tacccaaccg gaaattgccg
    17521 ttcctgctta tctggaacgc ttaaaattat tgttcaagaa aaatccgcag aatgccattg
    17581 aacaattgca gacctattta aaacaaacac ctaatgagct gatgaaagcc gaactttatt
    17641 atactttggg ctatttttac tttcaacaga aaaattatac cgaagcaata aggcagttag
    17701 gacaagccag aaattacgaa acaagcaggg aattgaatag caggattgat ttcctcattg
    17761 ctgaagcatt ttatttcgct ggcaatagta acttagcaaa agatgccttc aatcgttatc
    17821 tcagtcgcta tccatcagga aacaaagcag ataaggcata tttctatctc ggttatctca
    17881 gttttctgga aaaggattat actgaagcta aaaataactt tcaggaactt atcaatctct
    17941 atccggaatc gttttatggc aatgaagcgt tatattatct ggcggaaatg gatttttatt
    18001 tggcaaacta caatctggct ctgaaaaaat atctttattt atatgaaaag aatccggaaa
    18061 atgaagtgat tgccttacgc attgctcaaa tttatttcta tttaggcgat tatgaccaaa
    18121 gcgagaactt tttgcaaaac cttgttccca actacgatat ttgccttttg aaaggcaata
    18181 ttatgctggc aaaaaagaat tattctccgg ctttagaaca gtttattctt gcggagggct
    18241 ttgccactga taatgtgcgt aaaattgagg cacagagtta cagagctctc tgtttatatc
    18301 aaatgaaacg ctttaaagat gcctcaacct tatacctaaa actttcccgg gagaaagaaa
    18361 gtcctgatac ctatcttttc cttgctgcca aatctgctta tgctgcaaag gactatcatc
    18421 tggctttgga actatacaac aattttatag ataaatatcc cgagtcctct cattttctgg
    18481 aagcgcttac cgatattgcc aatacttatt ataatatggg caattatgaa cgagcggtaa
    18541 acgactggat aaatatttta acccgtttcc gcaatactac aaagtttact gaaaatcagc
    18601 tggctacaat tcacgatgct ttagttggct tggagctggc tttgaaacgg gtgaataaca
    18661 aagacgaact gttaaatgat cttctcgttc tacctgatac cttcttcagc gaatatatca
    18721 aatttgaact caattatatc ctaatgaagg tttatgctga tgaattaaaa tgggcagacc
    18781 tcattgctac cgctgaaaaa atcagaacgg aatttcccga acaaaagcac gaagacattg
    18841 aattgcttat ggctacggcg cttattgagc tgaatcaata tgctgaagcg gataccctgc
    18901 ttgcggaatt gtatgctcaa agcaaaaata acgaagcact actgaaatgg gcagaattgg
    18961 aatatattac cggtaactat gattccgctt tggaaaaata caagcttgcc tataaaaaca
    19021 atcccgatgg cgctacttgg gttaagcttc tggaatgttc cgaagccaat aactataagg
    19081 attttgacaa gctttggtct ttgggctcaa attatcttaa gaatcatccc gaagtaaatg
    19141 ttatccgtat gcgtcagctc tataatgcac agcgctttga agaagcagaa gctctcattg
    19201 aatttatcat caataacagt ttgtccactt tagaccattg taatgccttt ctggtaatgg
    19261 gaatgattga ttatcatcgg cattcctatc tttcagctat ctcaaccttg aaaaaggtga
    19321 ttttactttt ccctgaatat aaggaaattc gcagtaaagc aatttattat tccgtcttgt
    19381 ctttgctgga atccggtgca aaaaccgaag cagaggcatt atttttcaaa tatagagagg
    19441 aacttgatga ggcagcaaaa atggaactga cggatgtttt tttggaggta aatcaatgaa
    19501 acgcactttg ttaattctta ccctcgcttt aaccctaaca ctattgtttt ctcaaaccca
    19561 ggaacttccc gatttaacag ttagtggaga aagttccttc aaaccttttc tctataaacg
    19621 ctctttgctc ttttcaccgg agctcaatat gggagacagt ttacctgctt tcgttcctcc
    19681 gggagttcct ttacaggaaa aacccaagcc gattcccgct atgcaacatc gcagttatct
    19741 gcagtttgaa ggaaatatgg atttcggact taatagtttt atcagttatt atcccgatag
    19801 ctttatcctg aatgcattaa cttacactct ggatatgcgt tctccaattt ccgaaatgct
    19861 ttccgttcat aacaacctgt ttctgggaac cgagctctct tcccattttc cgctttcttt
    19921 ccgttttcag aatgtttcct cgcaagcgga tgattttgat aataccgttt tggattttaa
    19981 ttttagtcat cacagacccg acttgactat tggaaatgtt tctttcaagg atatttccgc
    20041 tcaattgggt tacaattata tctaccaaaa aaacctcaat aagccctaca aatgtaacta
    20101 cctgaatgta tacctcgctt cctgtctgga agaggaaatc ctgaattgga aaaccaaatt
    20161 tatggtaaaa tccggtgatt tcggaattca aattgcccct gtctataatg gtgatattat
    20221 ggacatttct aaacctggaa ttcatttact tgctgatgct tacactattg ttccttccct
    20281 ggaatttctc tatcgttatc ccataaccgg ctggggtgtt ttaagtttat ccaatgtgcc
    20341 tcttttggaa ggaaacgact atctttcctt tctggaagca acaccctgga tttctttcag
    20401 cgatgctcat aaactgaaaa agacaccgct gaacttcaat gcaggtcttg aatatctcta
    20461 tcctcaaaaa ataaatttct ccttaggcag attgaatatc aacaataacc tgcgctacga
    20521 aatagatagc cctgttttag taagcaccaa taattatcgc attcccaccc ttcgctatac
    20581 cgatgtcttc tccaatttaa cttctgtaga agcatttttc aaaatggata aactcaatat
    20641 gcatcagggt ttggaactgg aacttgccta cttaaccgaa agcgattata cgagagcgcc
    20701 ctatcgtcca ctccttaatc tggactcgcg gtttgcctat ggctatggca actggtattt
    20761 taatctggat attttacagc actattttac taaagaccat cgtggacacg acctccccga
    20821 agctataatc ctcaatttag gtgctgaata ccataaggat aattccgctg cctatgctca
    20881 gcttgccaat ctattcaacc gaaaagaatg ggtcttttcg gaacagccag ggaaaggacg
    20941 taatctctat atcggcttga aacaccggtt ttaagattgg acagaattta acaaggaaac
    21001 ttggtagatt tatggttgag agggttgaga aagtttaaag ggttgagagg gttgagaaag
    21061 tttagagg
//
LOCUS       DAOOZV010000033        20183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_2790_length_20183_cov_60.747019, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000033 DAOOZV010000000
VERSION     DAOOZV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_20183_cov_60.747019"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PLW08_04620"
     CDS             complement(<1..1264)
                     /locus_tag="PLW08_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51920.1"
                     /translation="MKKILLTLVFTSVLLFILAVPRNLVVVEIATGTWCTYCPGAAMG
                     ADDLIANGQPAAIIENHTGDSFANVYSNARNSYYNPSGVPTAYFDGLNAVVGGDHTQS
                     MYSYYLPRVTSRIAVPSKYTISATGNLTGNNLTVAATVVKPEADTNTNVLLHCVITQS
                     HIQYSWQGQTHLNFVNRLMLPNQSGTAVTLATGEQQTYNLTGIFNPEWDINSCEAVLF
                     LQNNATKEILQGVKYSLPELLGINPISVTELNFPETYIGGSSSLSFTINNYFNFTVTG
                     TISSSNPVFIPEQTNFSIAAYQATTINVNFVPASAVQYTGILTLNSNLSGFNIVQIPL
                     SGTGFFNAPPSVSNLIIVGPPVIYQGLTVLYDFSDPDGNVEGNSRFQWMRMFNNQPQV
                     IQNAVQQTYIIQPEDMDYPIACQVTPVDQ"
     gene            complement(1302..2453)
                     /locus_tag="PLW08_04625"
     CDS             complement(1302..2453)
                     /locus_tag="PLW08_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL51921.1"
                     /translation="MNDKLTDIFNEIRVSYESTLGKRAFHSANAWRSKKEEPDIMRSQ
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNMIDEEMTNRNLHIAYVSQISRTIGKYLKLN
                     TELIEAIALGHDLGHCPFGHDGETALSQCCEKNGIGEFHHNIESLHIVDHISRKGKGL
                     NLTFQVRDGIISHDGEVHNTQLIPQTNKTEEDIQNYIYAKKAGQDLQFMPATLEGCIV
                     RITDTIAYIGQDIEDAIRFNILTRDELPAEQVTYLGNTNSQIIETLIKSVIANSYEQD
                     FIAFDKETSEHLLALKKFNYKRIYTDENVKKSNSIIYRTMPILFDIYLNDIQKNNRES
                     KIFKHFLNHKFPEYLENFSPAEKVRDFIATMTDRYYNEEIKAYLLPWRG"
     gene            2682..2936
                     /locus_tag="PLW08_04630"
     CDS             2682..2936
                     /locus_tag="PLW08_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51922.1"
                     /translation="MLYTLPDGRKIDLARVESISKIREYGQAGQIIEEQFIGFAIHLK
                     NNETIEIKDKYHYSDWSEVKIRLSKLRDDIVTQWEKVQKK"
     gene            3156..5267
                     /gene="fusA"
                     /locus_tag="PLW08_04635"
     CDS             3156..5267
                     /gene="fusA"
                     /locus_tag="PLW08_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL51923.1"
                     /translation="MDTDADSPLTKIRNIGIMAHIDAGKTTTTERILFYTGYLHKMGE
                     VHDGNTFTDWMEQERERGITITSATVTCYWNAYQINIIDTPGHVDFTAEVERSLRVLD
                     GAIGIFCAVGGVEPQSETVWNQANRYRVPRLAYINKMDRVGADYFHTIQMIRERLTPN
                     AYPVNMPMGREENFEGVIDLITMQAYYFDPLTFGYEVKQTTIPEKYLESAKSMRDELL
                     EKVSEFSDELLMLFLEGEEIPVSVLKEAIRKGTISSQFVPVFCGSSLKNKGIQLLLDG
                     ICDFLPSPLDIGDTQGFEPVTHNPVNISPDPKGPLVALAFKVQIDKYIGRLVYIRVYS
                     GTLKKGGSFVNQANGKKERVSRILQMMSNRKNDLDCLHAGDIGAIVGSRFLITGDTIT
                     DGNFEVLLSKMHFPDTVISMAIEPKTKADQENLDIALMRLEEEDPTFRVHTDKDTGQT
                     LISGMGELHLEVIVDRLRREFGVAVNQGNPQVSYKETITKEVEAEEVFQRELNGKGNY
                     AYVKFRLSPIALKDLPEDAKNIFVNKISEDKIPSEFWKPIEEAVFSALNDGPLISGNV
                     ERVCIELIDGDYNPVDSNELAYRIATGIAISKGLRDASPVIMEPIMLLTVIAPDEFVG
                     DILNDINAKRGKIEIMRRENEFKQEIVAEVPLSELFGYATRIRSLSQGRAIYTLEFKK
                     YEICPVQVQNAILKRIRGYVE"
     gene            5324..5632
                     /locus_tag="PLW08_04640"
     CDS             5324..5632
                     /locus_tag="PLW08_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPL51924.1"
                     /translation="MNIELSLDGKVAKMKIDGILNSENAYLLQEKLTEVLNSDANLLE
                     LDLLDCRYISSTGIGKILLFYKDFITKGGEIEVVRSSNSVYELFSMLKLNQLFTVNLG
                     "
     gene            5634..6548
                     /locus_tag="PLW08_04645"
     CDS             5634..6548
                     /locus_tag="PLW08_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL51925.1"
                     /translation="MKHRILVVDDSSSLVSILSELLKVNGFEVETALSGTEALHKIES
                     EDYDLVICDIEMPGMSGLEFLSRVRRDYEKDIDFILMTGYADNEYFIEAIRLGASDFI
                     HKPIDSKQMMRSIQTILKRKKKRSDFSEFYNNLEKAEFHFELDPKNFSKFAVSEVFHK
                     FLRQNFDLTPNALNEILICIDEMVYNAYIHGTLGLNVQERVMEHNALQKIINERLQQP
                     EIASKRMRLYFSLCYKTQTIKITVEDDGPGFDYETWIKRVANEPKLNLEENGRGIAML
                     YHLSDKLEFDREGRTVTISKKLPINNKK"
     gene            6545..7366
                     /gene="prmC"
                     /locus_tag="PLW08_04650"
     CDS             6545..7366
                     /gene="prmC"
                     /locus_tag="PLW08_04650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPL51926.1"
                     /translation="MILQELLEQAKSLALAQNIPETDFWFLISYYLHLSRSEIILSRQ
                     RILTDREREIIENAFSRLEKGEPPQYITGIAYFYGLDLKVNPAVLIPRPETERLVELT
                     IERLKGTERILDIGTGSGAIAIALKHNLPSLNVSATEISFSALETAKKNAEIYGADIH
                     FYLSDCFPPIKQSYEVLISNPPYISKAEFATLNSRIKDKEPVIALQGGEDGLDFYRKL
                     LSESSEYLSENGFLALEHSDTQKEAIMNLARKEGWTKIEPLKDLTDKDRYLFIYK"
     gene            7379..8632
                     /gene="murA"
                     /locus_tag="PLW08_04655"
     CDS             7379..8632
                     /gene="murA"
                     /locus_tag="PLW08_04655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPL51927.1"
                     /translation="MERFIIEGNRNLNGTIYVSGAKNAVLPAMSACLLTKGKSVLHNV
                     PDLIDIKTMSHLLRIIGARVDFEDGNMSIDSRDVCFPEAPYELVSKMRASIYVLGPLL
                     ARLKEARVSFPGGCAIGTRPIDLHLKAMETLGAEISVDYGYINAKAKELKGADIYFTK
                     SSVGATINALMVAVLAKGKTRIFNAAMEPEVDSTIDLLNKMGAKINGKGTTTLEIEGV
                     EELIPVEMNIIPDRIEAGTFLIAGALSKNPVTVANCESAHLTAVLEKLRESGCELEIE
                     DKAITVIPPFSIKPVNLITLPYPGFPTDLQAQFTVLMSLADGVSFIEDTIFPERYMHI
                     AELNRLQANIKIERNIAAVKGVKELSGAEVMATDLRASAALVLAGMVAKGTTIVSRIY
                     HIDRGYEHIETKLNSIGANITREEV"
     gene            complement(9187..12213)
                     /locus_tag="PLW08_04660"
     CDS             complement(9187..12213)
                     /locus_tag="PLW08_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL51928.1"
                     /translation="MKKIAIMLCFALLTIGLFATLEWTEAVAIRQGVNIEWFRTGIET
                     TDGGAIYVWSDTKLGERDLWAQKVDGAGNMVWGEPVLIDGKPDRQEDPVITRTSDNNY
                     VIAWIDFSADLDGDVYAQKIDGEGNLLWQEGGVPVCTLAGMQIALNMEPDNEGGVYII
                     WVDSRNPSKDLFGQRLSANGSPLWIVNGIPIATGLGDEMQNTMLPDGEGGMIIAYTHS
                     YAGNDDLYAKRFNANGTMVWQNTLVISEAEGSQSDIRMAALNDGNFVFTWADKRSADT
                     DIYAQKINLTGDLLWGSYLIVYSDQNGLARPQVNPRIVKTSDNGVIIVWEDFRLDTQN
                     PDLFAQKISASGIKQWSEEGIALCTAEFAQLGPRLASDNNGGCFVVWDDLRNGNAPNV
                     DIYAQHLSASGNALWEANGKAICIAPNEQSGSLIKVSNNIVFINWMDIRNGSVGIYYQ
                     ALTYEGTVLLAVNGAEVFWGLSGDAPLDNYLILKRSSDVVIIWQDTRFANDGYRLFFQ
                     FLNPDGSIDLEPNGHPVTVSVGGSQSTPSAVVTPDDQIAIIWEDARGDNPKVYAQLLS
                     ANGERLWGEQGMELTVNSPLRQKDPKVSYYNGSLYFGWSGSDQVETSFFYHIYGQRIY
                     NGQKMWGPNGILISTLAQSDLRNECTLYDLIDNYYVWHRINPALATQTIWVKRVAEDG
                     SAMDGWQEEGIQTSNYNDWDAIQLFPKAHKTPEGIFVMWRDLRNDYIQNYWGQHLAEN
                     GTYLWNPVGVNLNDNQREQEKASIVVNSSGITFAWCENINGMNDIMAQKYSFPGEPLW
                     TNSGYYVVQRDSAQSDPCLVYFPDGNYLISWADFLNIESDLYYKYITENGEMIGAPQG
                     NVLCNASKSQYQPQTAILNNKAYTVWADGRSSGKTEILGLYAQKLGNGTPVTDVTIPP
                     LQSLILHQNYPNPFNPETTINFTLKDPTLSLKLNIYNIKGQLVKTLYDGALQKGQHSF
                     VWNGTDETNCQVSSGVYFYRLSNGKESRQRKMVLMK"
     gene            12683..13630
                     /locus_tag="PLW08_04665"
     CDS             12683..13630
                     /locus_tag="PLW08_04665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPL51929.1"
                     /translation="MFSKLILITGNANRPLAEEVSRHAGIPLADIDLFKFSNDESFVK
                     INDNVRGADVFVIQPTCYPVNDNLIDLLIIIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLVADLITIAGAHRVITVDLHADQIQGFFNIPVDHLYAIPTFARYFKSIM
                     PMDDIVVVSPDSGGANRARALAKRLNCSLAIGDKRRSGNEDRAELLNIIGEVNNKTVI
                     LFDDIIDTGGSLIKVANALVDKGVKKIYAACTHGVLSGNAISDLEASPIEKLFITNTI
                     PLSEEKKQCTKIVQLSIAEMLAIAIKKVHIEESISVLFR"
     gene            13646..14263
                     /locus_tag="PLW08_04670"
     CDS             13646..14263
                     /locus_tag="PLW08_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPL51930.1"
                     /translation="MIFNLKATPRKTIRKSDLHTLRASGMIPAVIYGPELESISVSIP
                     KGDFTQLYKKSFAEVSFWDIELNGDQFHTILKDKQIHPVTREMLHLDFMVVSAGAIIE
                     VEVPIIITGEPIGVKEGGMMEIVQRNVKISCKANEIPDELTLDVSNLKIGESLHIRDL
                     PQGNWQVKEHSDVTLVAIHAPRAEVVEEKPAAPAPEEQTTPETEK"
     gene            14273..14869
                     /gene="pth"
                     /locus_tag="PLW08_04675"
     CDS             14273..14869
                     /gene="pth"
                     /locus_tag="PLW08_04675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPL51931.1"
                     /translation="MKLIIGLGNPGKEFANNRHNLGFICLDRWALGRERSFAKGDVFD
                     YIVLKRAVLIKPKTWMNRSGEAIKQALKQWIISEYMVIYDDIELPVAKIRIRQGGGDG
                     GHNGIKSLLAVIPPEELKRIRIGIGRNNEIDPQNYVLSDILPEEWELLNPALDLAGKF
                     IDLYIKYDFNAVLNEYSIWKKSCSGAESSGIVSPKEEK"
     gene            14869..15156
                     /gene="rpsF"
                     /locus_tag="PLW08_04680"
     CDS             14869..15156
                     /gene="rpsF"
                     /locus_tag="PLW08_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPL51932.1"
                     /translation="MTKDYELMVILVPTLSADEANAMNDSLMALVTENGGEIIKIDPW
                     GKRMLAYPIKKFREGYYFVDYFRVDSLVIKKLKALFNINENIIRYLFVAKN"
     gene            15166..15609
                     /gene="ssb"
                     /locus_tag="PLW08_04685"
     CDS             15166..15609
                     /gene="ssb"
                     /locus_tag="PLW08_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPL51933.1"
                     /translation="MADLKVPRLNKCIFSGRIGNDVELKYTPKGVPVVRFVVACDRRY
                     KDETEQWQTATSWIDCVAWRKWAEFIANQTHKGSAVIVEGRIETRNWTDQNNQNRKTT
                     EVIVDVLHSLEWKPREEGATPPNEEVPLPAEETLHHNTTTDDVPF"
     gene            15619..15855
                     /gene="rpsR"
                     /locus_tag="PLW08_04690"
     CDS             15619..15855
                     /gene="rpsR"
                     /locus_tag="PLW08_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPL51934.1"
                     /translation="MNTSDRKKKYTRRKYCRFCANKEIQIDYKNIDVLKPYISEVGKI
                     EPARLTGTCSKHQRKLTTEIKRARQMALIPYVIE"
     gene            15856..16692
                     /locus_tag="PLW08_04695"
     CDS             15856..16692
                     /locus_tag="PLW08_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51935.1"
                     /translation="MPIYPVIFGALSLLSPFLGLILYMLYVAKTAELNLTKPLQAFIM
                     FIPIPVLLLIFDPQTSTRLLCLDAILAVGLPVLVFLIVLKNNHILSNSFLAAFLVMFA
                     WGMLRYYLFKTYQDQLFEQGMQVVKDKMPALLNNDLLQQTLPFWKGIIPSIWVISQAI
                     AWLIGFLLFEGRLQIPNRIENLRFPVYFNLLIIAVLPLYFFDQTKLLFYNLLLSLCII
                     PFFQGAGLVWQRLGMIFSNRIISGLFMLIIVLYANILLVLLGFGNMWFTKRNITPGGY
                     TE"
     gene            16689..17135
                     /gene="rplI"
                     /locus_tag="PLW08_04700"
     CDS             16689..17135
                     /gene="rplI"
                     /locus_tag="PLW08_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPL51936.1"
                     /translation="MKVIMLKTIEKVGNQGDVVNVKRGYARNYLVPRGFALYATPANL
                     KNLNAIKNKLADEEQKLTEELKNLAEKIDSTKLVFMRKVDEHGTMFGSVSELDIIRSL
                     EEQGINIQKNDLEMDKHFKELGEFQVPIHLRKDIVCTLNISIEPEQ"
     gene            17154..17729
                     /locus_tag="PLW08_04705"
     CDS             17154..17729
                     /locus_tag="PLW08_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51937.1"
                     /translation="MSDLWFKIKQIITLVVFVAVLSLLGMISGRPIMIVAYGVFFLVV
                     VAIMFYMTRKRQRHFEKVKGSSQLFRKIFGILLMILALITPPVIILRTNLITLPETIK
                     SGAALGIVSGVTVLFIALTLLAVYFINYRGSEVSNRVIGYILYIIAAIVPGFLMSRVE
                     KTTIGIGSVYYVALIVLILSYSGFGLLSNKE"
     gene            17799..18113
                     /locus_tag="PLW08_04710"
     CDS             17799..18113
                     /locus_tag="PLW08_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPL51938.1"
                     /translation="MAFSSFSRLSENLIYRLGGDKYRSFIHIYLSWSKIVGEILASQS
                     HPVKVENGVLFVAVQNNTWMQELVLIKDDIIAKYKSIYEEEIADIVFLISSPKRKCKR
                     RK"
     gene            18095..18748
                     /gene="rpe"
                     /locus_tag="PLW08_04715"
     CDS             18095..18748
                     /gene="rpe"
                     /locus_tag="PLW08_04715"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPL51939.1"
                     /translation="MQKTQIAPSLLAADFGNLDSEITSLEAAGADILHLDIMDGHYVP
                     NLSFGFPVIRTIRFLSKLPLDVHLMVTNPDFHISLLLDMDVQWVSFHQETFYHSHRLI
                     QTLKEKGIKAGLALNPGTPIGTLEALLPDLDYILVMSVNPGFSAQDFIPSAIYKIWDL
                     YNLRKKNNYRYLIEVDGGVTAANAKALKEAGTDILVSASYIFSSHDYSQAISALRNA"
     gene            18767..20107
                     /gene="ffh"
                     /locus_tag="PLW08_04720"
     CDS             18767..20107
                     /gene="ffh"
                     /locus_tag="PLW08_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPL51940.1"
                     /translation="MFENLSERLDKIFRNLRGKGYLTEDNIKAGLREVRMALLEADVN
                     FRIVKNFIAEVEKRALGVEVMKSLTPGQQVVKIVYEQLVNLMDTEGFQFKVYNNRLTK
                     VMLVGLQGSGKTTACAKLAAFYRKKGIKPMLVACDVYRPAAVDQLKILGKQIGIPVVS
                     EDTKNVLKIADSALSMAEKEMVNLLIFDTAGRLHIDEPMMEEVARLKTHIKPDYIFFV
                     ADATTGQDAVTIAKDFYDKLAFDAVILTKLDGDARGGAALSIKAVTGKPVAFVGIGEK
                     ISDLEIFYPDRMASRILGMGDVLTLIEKAEEAIDMEAEEKLARKMLKNQFTLNDFLQQ
                     LQSIKKMGSLESIIRMIPGLGRKLPADLKIDDKALKHIEAIIQSMTEKEREHPEIING
                     SRRLRIAKGCGLSVTEVNRLLRQFEEMKKMLKKVNTPQGRKHIERMMGGNRPEI"
BASE COUNT         6359 a         3617 c         4117 g         6090 t
ORIGIN      
        1 attggtcaac aggagttact tgacaggcaa ttggataatc catatcttca ggttgaataa
       61 tataggtttg ttgaacagca ttttgaatca cctggggctg attattgaac attctcatcc
      121 attggaacct gctattgcct tcaacattgc catcgggatc agagaaatca taaagaaccg
      181 taagcccctg gtaaattacc ggtggaccta ctattatcaa attgctgaca gaaggaggcg
      241 cattaaaaaa accggttccg gaaaggggaa tttgcactat attgaaaccg ctcagattgc
      301 tgttgagtgt taaaattcct gtatattgaa cagcagaagc aggaacaaaa tttacattta
      361 tagtggtggc ttgataagca gcaatactaa aattggtttg ctcaggaata aaaacaggat
      421 tggatgaaga aattgtgcct gttacagtaa aattaaaata gttatttata gtaaaagaca
      481 agctgctgct tccgccaata taggtttcag ggaaattaag ttctgtaaca gaaataggat
      541 taatacctaa gagttcaggc aaagaatatt taactccttg caatatttct ttggtagcat
      601 tattctgcag aaaaagaact gcctcacagg aatttatatc ccattcagga ttaaatattc
      661 cagtaagatt ataggtctgc tgttcaccag ttgcaagagt tactgctgtt ccgctttgat
      721 tgggcaacat taaacggttt acgaaattca aatgtgtttg accctgccag gaatattgaa
      781 tatgtgattg ggtaataaca caatgcaaaa gcacattagt attggtatca gcttccggct
      841 tgacaaccgt agcggcaaca gttaaattat ttccagtcaa gtttccagtt gccgaaatag
      901 tatatttaga aggaacagca atgcgggaag ttactctggg aaggtaataa gaatacattg
      961 attgagtatg atcaccaccc accacagcat ttaatccgtc aaaataagca gtaggaacgc
     1021 cactgggatt gtaataacta tttcgggcat tagaatagac attagcaaag ctatcacctg
     1081 tatgattttc aataattgcg gctggttgac catttgcaat taagtcatca gcacccatag
     1141 cagctccagg acaataagta caccaggttc cggtagcaat ttccactaca accaaatttc
     1201 ggggaactgc caaaataaag agcagaacac tggtaaaaac gagggttaag agaatttttt
     1261 tcattattac tccttatgat tagttaattg aaaaacattg tttatcctct ccaggggagt
     1321 agatatgcct taatttcttc gttgtaatag cggtctgtca tagttgctat aaaatcccgc
     1381 actttttcag ccggcgagaa attctccagg tattcaggaa atttgtggtt caggaaatgt
     1441 ttgaatatct tagattcccg attatttttc tggatgtcat ttaagtaaat atcaaaaagg
     1501 ataggcatag tgcggtaaat aatggaattt gattttttca cattttcatc ggtgtagatt
     1561 cttttgtaat tgaatttttt caaagccaaa agatgctcac tggtctcttt gtcaaaagca
     1621 atgaaatcct gctcgtagct atttgcaatc acgcttttga tgagtgtttc tataatctga
     1681 ctattggtat tgcccaaata agtaacctgc tctgcaggaa gttcatcgcg agtaagaata
     1741 ttaaaacgaa tggcatcctc tatatcctgt ccaatatagg caattgtatc ggtaatacga
     1801 actatacatc cctccagagt tgcaggcata aattgaagat cctgcccggc ttttttggca
     1861 taaatgtaat tctgaatatc ctcctcggtt ttatttgttt ggggaattaa ctgggtgtta
     1921 tgcacttctc cgtcgtggga aatgattcca tctcgcactt gaaaagttaa attcagtcct
     1981 tttcccttgc gggagatatg atccacaata tgtaaacttt caatgttatg atgaaattct
     2041 ccgataccgt ttttttcaca acattgggaa agagcagttt ctccatcgtg gccaaaagga
     2101 caatgcccca aatcatgacc taatgctatc gcttcaatca actccgtgtt taacttcaaa
     2161 tattttccta tagtgcgcga gatttgagaa acataagcaa tgtgcagatt gcgattggtc
     2221 atttcttcat caatcatatt ggtgaaagaa aaaacctgtg tttttccatg atacctgcga
     2281 taagcgccac tgtaaaggat tctatctcta tccaccgcaa attgggaacg cataatatcc
     2341 ggttcttctt ttttgctgcg ccaggcattt gcagaatgaa atgcgcgttt gcccaaggtg
     2401 ctttcgtagg aaactctgat ttcgttgaag atatcagtca gtttatcatt cattactttc
     2461 tccctattac tctttaatga tataatattg caaaaaggtc tgctgtcaag aataacttgg
     2521 aatctttatc aacaacacgg attaaaagga tttttaagga ttcataggga ttttcatttt
     2581 tccattctta atcagaattt aaaagggcta aaataaatat tgacaaaatg atttgatata
     2641 ataatatgta attttatgag attcatagga aggttacaga aatgctttat acattacccg
     2701 atggcagaaa aattgatttg gcaagggtgg aaagtatctc taaaattaga gaatatgggc
     2761 aagcaggtca gattattgaa gagcaattta tcggatttgc tatacatttg aagaataatg
     2821 aaacaattga aattaaagat aaataccatt atagtgattg gtcggaagtt aagatacgac
     2881 tgagtaaatt gcgagatgat atagtaactc aatgggagaa agtccaaaag aaatagccgt
     2941 gtttgttttg tattaatcaa gtggtaaatt catttttact gctgagcatt tagttctcta
     3001 caataattat tctttttgct attgcccaat tggaattttt ccacttaata ctttatccct
     3061 cctagcttgt taaccttcag agctcttgga ctttttcata gacaaataag ctaaccctgt
     3121 ttttttggtt aataaattaa aaaaaggatt tatttatgga taccgatgcg gatagtccgc
     3181 ttactaaaat acgcaatatt ggtataatgg ctcatattga tgccgggaaa acaacaacta
     3241 cggaacggat attgttttat acaggttatc tgcataaaat gggggaagtg catgatggaa
     3301 atacatttac ggattggatg gaacaggaaa gggaaagagg aattacaatc acttctgcaa
     3361 cagtaacttg ttattggaat gcctatcaaa taaatattat tgatactcct ggtcatgtag
     3421 attttacagc agaagtagaa cgttccttac gggttttgga tggagcaata ggtatatttt
     3481 gtgcagttgg aggtgtggaa ccacaatcag aaactgtctg gaatcaggca aatcgttatc
     3541 gtgtgcctcg tttagcatac ataaacaaaa tggatcgggt aggcgcagat tattttcata
     3601 ccatacaaat gattagagaa cggcttactc ccaatgctta tcctgtaaat atgccaatgg
     3661 gcagggaaga aaactttgaa ggtgtaattg atttaatcac gatgcaggca tattattttg
     3721 atcctttaac ctttggctat gaagtgaaac agacaaccat accggaaaaa tatctggaaa
     3781 gcgcaaaaag tatgcgggat gagcttttag aaaaggttag tgaattcagt gacgaattac
     3841 tgatgttgtt tttagaggga gaagaaattc ctgtaagtgt attaaaagag gcaattcgca
     3901 aagggacaat cagcagtcaa tttgtcccgg ttttttgtgg cagttcctta aaaaataaag
     3961 gtattcagct tcttttggac ggaatttgtg attttctacc ttcacctctt gatattggag
     4021 atacacaagg ttttgaacct gtaacccata atcctgttaa tattagtcct gatcccaaag
     4081 gacctctggt agctttagct ttcaaggtgc agatagataa atatattgga cgccttgttt
     4141 atatcagggt ttattccgga accttgaaaa aaggcggttc ctttgtcaat caggcaaatg
     4201 gcaagaaaga aagagtttcg cgtattctgc aaatgatgag taaccgtaaa aacgatttgg
     4261 attgccttca tgcaggtgat attggagcta ttgtaggttc cagatttcta ataacgggag
     4321 atacgattac cgatggtaac tttgaagttt tgctttccaa aatgcatttt ccggatactg
     4381 taatttctat ggctattgaa ccaaaaacca aagcggatca ggaaaatctg gatattgccc
     4441 taatgcgttt ggaagaagaa gaccctactt tccgggtgca taccgataaa gatacgggac
     4501 agacattgat ttcaggaatg ggcgaattgc atttggaagt tattgtagat cgtttgcgtc
     4561 gggaatttgg cgttgcagta aatcagggta atccccaggt ttcctataaa gaaacgatta
     4621 ctaaagaagt agaagcagaa gaagttttcc agcgtgaact gaatggcaag ggaaattatg
     4681 cttatgtaaa attccgctta agtccaattg ctttaaaaga cctgccggag gatgccaaaa
     4741 acatctttgt aaataaaata agtgaagata aaatcccgtc ggaattttgg aaacctattg
     4801 aagaagctgt tttcagtgct ttgaatgatg gacctctcat tagtggtaat gtagaaagag
     4861 tgtgtataga acttattgat ggggattata accctgtgga ttctaatgaa cttgcctatc
     4921 gcattgcaac aggaatagct atcagtaaag ggcttagaga tgcctctccg gtaattatgg
     4981 aacctattat gttgcttaca gtaatagctc ctgatgaatt tgtaggtgat attttaaatg
     5041 atatcaatgc caaacggggg aaaatagaaa ttatgcgcag ggaaaatgaa tttaagcagg
     5101 aaattgtagc agaagttcct ctttccgaac tttttggtta tgcaacacgc attcgttctt
     5161 taagccaagg cagggctata tatactttgg aatttaagaa atacgagatt tgtccagtac
     5221 aagtacaaaa tgccattctg aaaagaattc gcggttatgt agaataaata cttgacagac
     5281 aaaaggatat tttagataaa gaaaacaaaa ggaggttata aagatgaaca ttgaactttc
     5341 ccttgatggc aaggtcgcta agatgaagat tgatggtatc ttaaatagcg aaaatgctta
     5401 tttgcttcag gaaaaattaa ctgaagttct taattccgat gccaatcttt tggaacttga
     5461 tttgctggat tgccggtata taagttctac agggattgga aaaattttgt tgttttacaa
     5521 ggattttatt acaaaaggag gagaaattga ggtggttaga agttccaata gcgtatatga
     5581 gttattttct atgctcaagc taaatcaatt atttacagtt aatctgggct gaaatgaaac
     5641 accgcattct tgtagtggat gacagtagtt cccttgtttc catcttgtcc gagttgttaa
     5701 aagttaatgg ttttgaagta gaaacagctc tcagtggaac ggaagcattg cataaaattg
     5761 aaagcgagga ttatgatctc gtaatttgtg atattgaaat gccgggtatg agtggtctgg
     5821 aatttctttc ccgcgtgcgc agggattatg aaaaagatat agattttatc ttaatgacag
     5881 gttatgcgga taatgaatat ttcattgagg caatccgact tggcgcttcg gattttattc
     5941 ataaacccat agacagtaaa caaatgatgc gttccattca gaccattctg aaacgcaaga
     6001 aaaaacggag cgatttcagc gagttttata acaacctgga aaaagcggag tttcattttg
     6061 aacttgatcc gaagaatttc tccaaatttg ctgtttcgga ggtctttcac aaatttctgc
     6121 gccagaattt tgacctcaca cccaatgcgt taaacgaaat tctgatttgt atagatgaaa
     6181 tggtttacaa tgcttatatt catggaactc tgggcttaaa tgttcaggaa cgggttatgg
     6241 aacataatgc cctacagaaa ataataaatg aacgcctgca acaacctgaa attgcctcta
     6301 aaagaatgcg cctttatttt tctctttgtt ataaaacaca aacaatcaaa ataaccgttg
     6361 aagatgacgg acccggcttt gattatgaaa cctggataaa aagggttgcc aatgagccca
     6421 aactgaatct ggaagaaaat ggcagaggaa ttgcgatgct ttatcatctt tccgacaaac
     6481 tggagtttga cagagaaggc agaacggtta ctatcagtaa aaaactgccc ataaacaata
     6541 aaaaatgatt ttacaggagt tgctggaaca ggcaaaaagt ttagctttag cccaaaatat
     6601 tcctgagacg gatttctggt ttttaatatc ctattatttg catctcagcc ggagtgagat
     6661 tattttatcc cgacaacgga ttttaactga ccgggaaagg gaaataattg aaaatgcctt
     6721 cagccgtttg gaaaaaggag aaccacctca atatataaca ggaatagctt atttttacgg
     6781 tttagaccta aaggttaatc ctgcagtttt aattccgcgt ccggaaacgg aacggctggt
     6841 ggaattgaca atagaaagat taaaaggaac ggaaagaatt ttggatattg gcacaggtag
     6901 tggcgctatt gctattgctt taaaacataa tctaccttct ctaaatgttt ctgctacgga
     6961 gataagcttt tcggcattgg aaacagctaa aaagaatgct gaaatatatg gggcagatat
     7021 tcatttttat ttgtccgatt gttttcctcc tattaaacag agttacgaag tgcttatttc
     7081 caatcctcct tatatcagta aagcagaatt tgctactcta aattccagaa ttaaagataa
     7141 agaacccgta attgctttgc aaggtggaga agatggactt gatttttacc gtaaattact
     7201 ctctgaaagc tctgaatatc tttccgaaaa cggtttttta gctttagagc atagcgatac
     7261 ccaaaaggaa gcaattatga accttgcccg aaaagagggc tggacaaaaa ttgagccctt
     7321 aaaggacttg accgataagg atagatacct gtttatttac aagtaaaaag gataaaaaat
     7381 ggaacgattt attattgaag gaaaccgcaa ccttaatggc actatttatg taagcggagc
     7441 caagaatgca gttttacctg caatgtctgc ctgtctttta actaaaggga aatcggtgtt
     7501 gcataatgtt cccgatttga tagacatcaa aacgatgtcc catttattaa gaataatcgg
     7561 agccagagtg gattttgaag atggcaatat gagtattgat tcccgggatg tttgttttcc
     7621 agaagctcct tatgaattgg tcagcaaaat gcgcgcttcc atctatgttt taggacctct
     7681 tttagcgcgt ttgaaagaag ccagggtatc ttttcctggt ggctgtgcca ttggcaccag
     7741 acctattgac cttcatttaa aggcaatgga aaccctggga gccgaaatat ccgttgatta
     7801 tggctatatt aatgccaaag caaaagaact gaaaggcgcc gatatctatt ttaccaaatc
     7861 cagcgtagga gctacaatta atgctctgat ggtagctgtt ttggctaaag ggaaaacaag
     7921 aatatttaat gctgcaatgg aaccggaagt ggattccact attgacctcc taaacaaaat
     7981 gggagcaaaa ataaacggta aaggaactac tactctggaa attgaaggtg tggaagaact
     8041 cattccggtg gaaatgaata taattcccga tagaattgaa gccggaactt ttctcattgc
     8101 tggtgctctt agtaaaaacc ctgttacagt agctaattgt gaatcagcac atttaaccgc
     8161 agttctggaa aaattacggg aaagcggttg tgaactggaa attgaagata aagccataac
     8221 cgttattcca cctttttcca ttaagcccgt aaatttaata actcttcctt atcccggttt
     8281 tccaaccgat ttacaagctc aatttaccgt tcttatgtct ctggctgatg gggttagctt
     8341 tattgaagat acaatttttc ctgaacgtta tatgcacatt gcagaattga atcgtctgca
     8401 ggcaaatatt aaaattgaaa gaaatatagc tgccgttaaa ggtgtgaaag aacttagtgg
     8461 agcagaagtt atggctacag acctgagggc aagtgcagct cttgttttgg caggtatggt
     8521 agctaaaggc actactattg tttccagaat ttatcatatt gaccgcggtt atgaacatat
     8581 agaaacaaaa ctgaattcca ttggagccaa tattacccgc gaagaagtgt agccggagga
     8641 cgctgttcct ccgctaaaaa ttaaaaaacg gagctacggc aagctccgat tacacagtaa
     8701 agtgaggact catagctact atgttggaag aatgaaaaat gaagcataaa tgtaaatcgg
     8761 cagtcagaat aagatttaca atggaacttg ctatccccga atgcattact tacccattgc
     8821 ttacccaata cggatggcat tagtaatggg taagcaatgg gtaagcaatg ctattaggga
     8881 aggcggcgga aaacggtata atagcttgga atataaacaa atatatagtt aatgccaaaa
     8941 taaactgtaa aattcaactg taacaatcgt gaaaccttgg gttgccgaat tccgattcgg
     9001 taaatacgaa ggataaagtt tctttagata actatatcat cttgggcaaa tcggaatttg
     9061 ccaacccatt tttgctttaa tatccatatt caaatttaac tctcacccca agctccaaag
     9121 ccctttttcc ctcttaatct caagaccttt ttacttcttg acctctcttc ctcttgaccg
     9181 ctctacttat ttcatcagca ccattttccg ttgtctgctt tcttttccgt tacttagccg
     9241 gtaaaaataa actccgctgg agacctggca attcgtttca tccgttccat tccagacaaa
     9301 actatgctgt cctttttgca gcgctccatc ataaagggtt tttaccaatt gcccttttat
     9361 attatagata ttgagtttta gggaaagggt aggatctttc aaagtgaagt tgattgtagt
     9421 ttcaggattg aagggattgg gataattctg gtgtaaaatc aaactttgca acgggggtat
     9481 tgttacatcg gtaacaggtg ttccattacc caatttttga gcataaagtc ccaaaatttc
     9541 ggtttttccg ctggaacgtc catctgccca aacagtatat gctttgttat tcagaatagc
     9601 tgtttgaggt tgatattgcg atttagaagc attacaaaga acattgccct gaggagctcc
     9661 aatcatttct ccattttcgg tgatgtattt ataataaagg tcactttcta tattcaaaaa
     9721 atcagcccag gaaataagat aattaccatc gggaaaataa acaagacagg ggtctgattg
     9781 agcggaatcc ctttgaacaa cataatagcc gctattggtc cataaaggtt cacccggaaa
     9841 agaatatttc tgagccatta tatcattcat tccgtttatg ttttcacacc aggcaaaagt
     9901 tatgccactg ctgttaacaa caatagatgc cttttcttgt tctctttggt tatcgttaag
     9961 attaacccct accggattcc ataaataagt tccgttttcc gcaagatgct gtccccaata
    10021 attctgaata tagtcgttac gtaagtccct ccacattaca aaaatgcctt cgggtgtctt
    10081 atgagctttc ggaaacagtt gaatagcatc ccaatcgtta taattagaag tttgaattcc
    10141 ttcttcctgc catccatcca tagcggaacc atcttctgcc actcttttca cccaaatagt
    10201 ttgtgttgcc agagcgggat taattctatg ccaaacatag taattatcta tcaggtcata
    10261 aagagtgcac tcatttctta aatcactttg agcaagggta gaaattagta ttccattcgg
    10321 tccccacatt ttttgtccgt tataaattcg ctgtccgtag atatgataaa agaaactggt
    10381 ttccacttga tcggaaccgg accagccaaa atatagggaa ccgttatagt aacttacttt
    10441 cgggtctttt tgccttaaag gactattaac ggtaagttcc ataccctgct ctccccataa
    10501 tctttctccg ttggcactaa gaagttgagc ataaacctta ggattatcgc ctctggcatc
    10561 ttcccaaata atggcaattt ggtcatcagg agttacaact gcactgggag tggattgtga
    10621 tcctcctacg gaaacagtta caggatgtcc attgggttcc aaatcaatac tgccgtcagg
    10681 attaagaaac tggaaaaaaa gacgatagcc gtcattggca aaacgggtat cctgccaaat
    10741 aataacaaca tcggaactgc gttttaaaat caaataatta tctaaaggtg cgtctccact
    10801 taaaccccaa aagacctcag ctccatttac tgccagaaga acagtgcctt cataagtaag
    10861 cgcttgataa taaataccaa cacttccatt acgaatatcc atccagttga tgaaaacaat
    10921 attattactt actttaatta aactgccgct ttgctcattg ggagctatac aaattgcctt
    10981 gccattagct tcccataaag catttccact ggcagaaaga tgctgtgcat aaatatctac
    11041 attgggagca tttccatttc ttaaatcgtc ccaaacaaca aaacaaccgc cattattatc
    11101 agaagccaga cgaggaccta attgagcaaa ttctgccgtg cataaagcaa ttccctcttc
    11161 actccattgt ttaatcccgg aagcagaaat cttttgcgca aaaagatcag gattttgagt
    11221 gtccaaacga aaatcttccc aaacaatgat tactccatta tcggaggttt ttacaatacg
    11281 aggattcact tgaggcctcg caagcccgtt ttgatcactg taaactatta aataactacc
    11341 ccaaagcaaa tcccctgtca ggttaatctt ttgggcataa atatcggtat cggcactgcg
    11401 tttatcagcc caagtaaaaa caaaattacc atcatttaga gcagccattc taatgtcgga
    11461 ttgacttcct tccgcttcac tgataactaa agtattttgc caaaccatag ttccattggc
    11521 attaaaacgc ttggcataaa gatcatcatt accggcataa ctgtgtgtgt aagcaataat
    11581 catccctccc tcgccatcag gaagcattgt attttgcatt tcatccccca agccagtggc
    11641 aataggaatt ccgtttacta tccataaagg actgccattt gcagaaagac gctgtccaaa
    11701 aaggtcttta ctcggattac gactatccac ccaaattata taaacaccac cttcattatc
    11761 gggctccata ttaagagcaa tctgcattcc cgccagagta caaactggaa ctccgccttc
    11821 ctgccaaagc aaattaccct ctccatctat cttttgagca taaacatcac catccaaatc
    11881 ggcagaaaaa tcaatccagg caataacata attattatca ctggttctgg ttattacagg
    11941 gtcttcctga cggtctggct tgccatctat taaaaccggc tctccccaaa ccatatttcc
    12001 tgcaccatca actttctgag cccaaaggtc tcgctcgcca agttttgtgt cagaccaaac
    12061 atatatggct ccaccatctg tggtttctat gccggtccga aaccattcaa tattcacccc
    12121 ttgacgaatt gccactgctt cagtccattc caaggtggca aaaagcccta ttgtaagcag
    12181 ggcaaaacaa agcattatcg ctatcttctt catttctatc tccttctttt aataaaaaaa
    12241 ctatttttta taccgctttt gggctataat acattgaaga atataacctt tcacttcaaa
    12301 tgcatttctc taatcatcct ccgcctcaat gctgaaaaac atttctcctt taacaactga
    12361 aaatatattc tccaccaagc atacatttat gcaagaattc ttccaaacaa gtttatattg
    12421 taaggggata aataagcttt gacaaaatga gcttgcttaa aaaagtggta aaacttgcct
    12481 gaatagacag gtagaaagca atgtaagata tagaaattat gttagttgat agaggaatta
    12541 cgagcttagg tattggataa tatatgaggt tatgggaaat aaaaattatc aggtaagaag
    12601 atatggtaag tgcatagccg caatggatat gcagaaagag gaaatttaac tggaacaagc
    12661 aaaaaaaaga gaagagagaa aagtgttttc taagctaata ttaatcacag gaaatgcgaa
    12721 tcgtcctttg gcagaagaag tttcccggca tgcgggaatt cctttggcgg atattgattt
    12781 attcaaattc tccaatgacg aatcgtttgt aaaaatcaat gacaatgtgc ggggagctga
    12841 tgtttttgta attcagccca cttgttatcc tgtgaatgat aatttgattg acctattaat
    12901 tattatagat gccttaaaga gagcttcggc acagcggata aattgtgtta ttccttatta
    12961 tgcttatgcg cgttccgata aaaaggatca gccgcgagtt cctattacag caaagctggt
    13021 agcggattta attactattg caggtgcgca cagagttatt actgtggatt tgcatgccga
    13081 tcagattcag ggttttttca atattcctgt ggatcattta tatgcgattc caacttttgc
    13141 ccgttatttt aaaagtatta tgccaatgga tgatatagtt gttgtttctc ccgattccgg
    13201 aggtgcaaac agagcaagag ctttggcaaa gcgcttgaat tgcagtttag ccattggcga
    13261 taaacgcaga agcggaaatg aagatagagc cgagctctta aatattatcg gtgaagtaaa
    13321 taataaaacg gttattttat ttgatgatat tatagacacg ggcggaagtt tgattaaagt
    13381 agctaatgcc ttagtagata aaggagttaa gaagatttat gctgcttgca cacatggtgt
    13441 tctaagcggt aatgcaatat ccgatctgga agcgtctcca atagagaaac tctttattac
    13501 caatacaata cctctttcag aagagaagaa acaatgcacc aaaatagtgc agttgtctat
    13561 tgccgaaatg ctggcaatag caattaagaa agtgcacatt gaagaatcaa tcagtgtttt
    13621 atttagataa taaatggagg aataaatgat atttaacctg aaagccactc ccagaaaaac
    13681 aatcaggaaa tccgatttgc ataccttgag agcgagtgga atgatacctg ccgtaatcta
    13741 cggacctgaa ctggaaagta tttctgtaag catccctaaa ggggatttta cgcagctgta
    13801 taagaaaagt tttgcggaag taagtttctg ggatattgaa ttaaatggtg atcaatttca
    13861 tacgattctg aaggataagc agattcatcc tgtcaccagg gaaatgttac atttggattt
    13921 tatggttgtt tctgccgggg caattattga agtggaagtt ccgattatta ttacaggaga
    13981 accaattgga gttaaagaag gtggaatgat ggaaatcgtg caacgcaatg taaaaatctc
    14041 ttgtaaggcg aatgaaattc ctgatgagtt gactttagat gtaagcaatt taaaaattgg
    14101 cgagtcactt catattcgcg atcttcccca aggtaactgg caggtaaaag aacacagcga
    14161 tgtaacatta gtagcaattc atgctcccag agcggaagtt gtagaagaaa aacctgctgc
    14221 tcctgctcct gaggaacaga caactcccga aacagaaaaa taatgcttat ttatgaagct
    14281 cattatagga cttggaaatc cgggaaagga atttgccaac aaccgtcaca atctgggttt
    14341 tatctgtctt gaccgttggg ctttaggcag agaaaggagt tttgccaaag gtgatgtttt
    14401 tgattatatt gttcttaaac gagctgtttt gattaaaccc aaaacctgga tgaaccgttc
    14461 tggtgaagca ataaaacagg cattaaagca atggataatt tcagaatata tggttattta
    14521 tgatgatata gaactgcctg tagccaaaat tcgtattcgt cagggaggag gagatggcgg
    14581 tcataatggc atcaaatcct tgcttgcagt tatacccccg gaagagctga aaagaattcg
    14641 gattggaatt ggcagaaaca acgaaattga tccccaaaat tatgtgctaa gcgatatttt
    14701 acccgaagaa tgggaattgc tgaatccggc tctggacctg gcgggtaaat ttatagacct
    14761 ttatataaag tatgatttca acgctgtgtt gaatgaatat agtatctgga aaaaatcctg
    14821 ttccggagca gaaagttccg gaatcgtaag tccaaaggag gaaaaatagt gacaaaggat
    14881 tatgaactaa tggtgatttt agttcccacc ttaagtgcgg atgaagctaa tgctatgaat
    14941 gatagtttaa tggctttggt aaccgaaaac ggtggagaaa tcatcaaaat tgacccttgg
    15001 ggcaagcgta tgctggctta tcccattaaa aaattcagag agggctatta ctttgtggat
    15061 tattttagag ttgatagctt ggttattaag aaactaaaag cactattcaa tattaacgaa
    15121 aacattattc gctacttatt tgtggcaaag aattgaggag aaaaaatggc tgatttgaaa
    15181 gtaccgcgtt taaacaaatg tatctttagc ggcagaattg gtaacgatgt agaactgaaa
    15241 tataccccta aaggagtccc tgtagttcgt tttgttgttg cctgtgatcg ccgttacaaa
    15301 gatgaaaccg aacaatggca aactgcaacc agttggattg attgtgtagc atggcgtaaa
    15361 tgggctgagt ttattgccaa tcaaactcat aaaggaagcg ctgtaattgt ggaaggaaga
    15421 attgaaactc gcaattggac agaccaaaac aatcaaaatc gcaaaactac cgaagtgatt
    15481 gtagatgttc ttcattccct ggaatggaaa cctcgcgaag aaggggcaac accgccaaat
    15541 gaagaagttc ctcttcccgc agaagaaaca ctccatcaca atactacaac tgatgatgtt
    15601 cctttttaga ggtaaagaat gaatacttcc gacagaaaga aaaaatatac ccgcagaaaa
    15661 tactgcaggt tttgcgccaa taaagaaatt cagattgact ataagaatat tgatgttctg
    15721 aaaccttata tctcagaagt aggaaaaatt gagccagcac gtttgaccgg aacttgttcc
    15781 aagcaccaaa ggaagcttac aactgaaatc aaacgtgcac gtcagatggc attaattcct
    15841 tatgtaattg aataaatgcc gatttatcct gtcatctttg gagctttatc actattaagt
    15901 ccttttctgg gtttaattct gtatatgctc tatgttgcca aaacggctga gttgaattta
    15961 accaagccct tgcaggcatt tataatgttt attccgattc cggtgctgct gttgattttt
    16021 gaccctcaaa caagtacccg tttactgtgt ttggatgcca ttttggcagt gggtttgccg
    16081 gttttggttt ttctaattgt gctaaaaaac aatcatattc tcagcaattc ctttctggca
    16141 gcatttttag taatgtttgc ttggggaatg ctgaggtatt atttattcaa aacctatcaa
    16201 gaccagcttt ttgaacaggg aatgcaagtg gtgaaggaca aaatgccggc tttgctgaat
    16261 aatgatttgt tgcagcagac cttacctttt tggaagggaa taataccttc tatttgggtt
    16321 atttcacaag caattgcctg gctgatcgga tttctgcttt ttgagggaag gttacaaata
    16381 cctaatagaa ttgagaactt aaggtttccg gtatatttta acctgcttat tattgcggtc
    16441 ttgcctttat atttttttga ccagacgaag ttgctgtttt ataacttgtt gctatctctg
    16501 tgcataattc ccttttttca gggagcaggt ttggtttggc agcgtttggg aatgattttt
    16561 tccaaccgta taatcagcgg cttatttatg cttattattg ttctttatgc caatattctc
    16621 ctggttctgt tgggctttgg aaatatgtgg tttacaaaaa gaaatataac tcctggaggt
    16681 tacacggaat gaaagttatt atgttgaaaa caatagaaaa agtgggaaat cagggtgatg
    16741 ttgttaatgt taaaagagga tatgccagaa attatcttgt tccccgcggc tttgctttat
    16801 atgcaactcc ggcaaactta aaaaatctta atgctattaa aaataagctt gccgatgaag
    16861 agcaaaaatt gaccgaagaa ttaaaaaacc ttgccgaaaa aattgactcc acgaaactgg
    16921 tatttatgag aaaagttgat gaacacggta ctatgttcgg ttccgtatcc gagctggata
    16981 ttatccgtag tttggaagaa cagggaatta atattcagaa aaatgacctg gaaatggaca
    17041 aacatttcaa agaactgggc gaatttcaag tgcctattca tctgcggaaa gatattgtgt
    17101 gcacgcttaa tatttctatt gagccggaac aataataaag ccgggaggtt tttatgtccg
    17161 atttatggtt taagattaaa caaataatca ctttggtagt gtttgttgcg gttttatccc
    17221 tattgggtat gattagcggc agacccatta tgattgttgc ttatggtgtc ttttttctgg
    17281 tggtggtagc cattatgttt tatatgacac gcaagcgtca gcgtcatttt gagaaggtta
    17341 aagggtcgtc tcaactcttt agaaagattt ttggaattct tctaatgatt ctggctttaa
    17401 taactccacc ggtaattatt ttgcgcacca atttgataac tcttcccgag acgataaaat
    17461 caggagccgc gttaggaatt gtaagcggag ttacagttct gtttattgct ttaacattat
    17521 tggctgttta tttcattaat tatcgtggga gtgaggtcag caacagagtt atcggttata
    17581 ttctttatat tatagcggct attgttccgg gatttttaat gagcagagtg gaaaagacaa
    17641 ccattggcat cggttccgta tattatgtag cgctgattgt tttaatcctt tcctacagcg
    17701 gtttcgggtt attatccaac aaggaataat cattttttga caggacatta taagttcacc
    17761 gaagtgtttt atcttcggtg gagagtttta ttttagcaat ggctttcagc tctttttccc
    17821 ggctttccga aaaccttatc taccgtttag gcggagataa ataccggtct tttatccata
    17881 tttatctttc ctggagcaag atagttggcg aaattctggc ttcccaatcg catcctgtaa
    17941 aagtggaaaa cggtgttctt tttgttgctg ttcaaaataa cacttggatg caggaacttg
    18001 ttctgattaa agatgatata attgccaaat acaaatctat ctatgaggag gagattgctg
    18061 atattgtttt tcttattagc agtcccaaac gcaaatgcaa aagacgcaaa tagccccttc
    18121 actcttagca gcggattttg gaaatcttga ttccgaaatt acttctctgg aagccgctgg
    18181 agccgatatc ctgcatttgg atattatgga tggtcactat gtgcccaatt tgagtttcgg
    18241 gtttcctgta attagaacta tccggttttt aagtaagctg cctttggatg ttcatttaat
    18301 ggttaccaat ccggattttc atatttccct tttgctggat atggatgtgc aatgggtcag
    18361 ctttcatcag gaaacatttt atcatagtca ccggcttatt cagacattaa aagaaaaagg
    18421 cattaaagcc gggttggcgt taaatcctgg aactcccata ggaactttgg aagctctttt
    18481 gcctgatttg gattatatat tggtgatgag tgtaaatccc ggtttttcgg ctcaggactt
    18541 tattccttct gccatctata aaatttggga tttatataac ctgcggaaaa agaataatta
    18601 ccgctatctc attgaagttg acgggggagt tactgccgct aacgctaaag cattaaaaga
    18661 agcgggaacc gatattctgg tttctgcctc ctatattttc agcagtcacg attatagtca
    18721 ggcaatttct gctttaagaa atgcctgaac atattaagga tcaataatgt ttgaaaacct
    18781 atctgaacgc ttggataaaa ttttccgtaa cctgcgggga aaaggatatt taaccgaaga
    18841 taacataaaa gcgggtttgc gcgaagtccg aatggcgctt ttggaagccg atgtaaactt
    18901 cagaattgtg aagaatttca ttgctgaagt ggaaaaacgg gctttgggtg tagaagttat
    18961 gaagtccctt accccgggtc agcaggttgt aaaaatcgtt tatgaacagc ttgttaacct
    19021 tatggatacg gaaggttttc agtttaaggt ctacaacaat cgcctcacta aagttatgct
    19081 ggttggtttg cagggttcgg gaaaaacaac tgcctgtgcc aaacttgccg ctttttaccg
    19141 gaaaaaaggc atcaaaccga tgcttgttgc ctgtgatgtt taccgtcctg cagctgttga
    19201 tcagcttaaa attctgggta aacaaattgg tattcccgtc gtttccgaag ataccaaaaa
    19261 tgttcttaaa atagcggatt ccgctttatc tatggcagaa aaggaaatgg ttaatctgct
    19321 tatttttgat actgcaggcc gtcttcatat tgatgaaccg atgatggaag aagtggcacg
    19381 cttgaagaca catataaaac cagattatat attttttgta gcagatgcaa caaccggaca
    19441 agatgccgtt acaattgcca aggactttta cgataaactt gcctttgatg ccgttatttt
    19501 aactaaactg gatggcgatg cccgcggtgg agcagcttta tccattaaag cagttacggg
    19561 aaaaccggtt gcttttgtcg gtatcgggga aaaaatcagc gatctggaaa ttttttatcc
    19621 cgatagaatg gcaagccgta ttttgggaat gggtgatgtt ttaaccctta ttgaaaaagc
    19681 cgaagaagca atagatatgg aagcggaaga aaaacttgcc cgcaaaatgc tgaaaaatca
    19741 gtttacttta aatgatttcc tccaacaatt acaaagcatc aaaaaaatgg gttctctgga
    19801 atcaattatc cgtatgattc ctggcttggg gcgcaaactt ccagccgatt tgaaaattga
    19861 cgataaagca ctaaaacata tagaagcaat tattcaatca atgaccgaaa aagaacgcga
    19921 gcatcctgaa attattaacg gcagcagacg tctccgtatt gctaaaggat gtggacttag
    19981 cgttacggaa gttaatcgct tgttacgcca gtttgaagaa atgaaaaaaa tgctgaaaaa
    20041 agtcaatacc cctcagggaa gaaaacatat agaacgaatg atgggaggaa atagaccgga
    20101 aatttaatca atagcttcgt gatgaacagg attacccgat tttgaatgat gaatgaaaaa
    20161 taaagaatca atataaacag gaa
//
LOCUS       DAOOZV010000034        18860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3028_length_18860_cov_58.380218, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000034 DAOOZV010000000
VERSION     DAOOZV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18860
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_18860_cov_58.380218"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2513
                     /gene="bamA"
                     /locus_tag="PLW08_04725"
     CDS             261..2513
                     /gene="bamA"
                     /locus_tag="PLW08_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPL51941.1"
                     /translation="MRRIILCSILILLLGCLGQNLYAVGETIYEIKVIGAVNIDPELV
                     TSALSFRVGDTLDPEAVAKSIRNLYRMGIFSDIQVESEPYRTGVTILIKIQENPIVSS
                     IEYIGFKAVKQERIDELVNVKVGSYWSEGVKNQLLNKLKNEYATKGFSNAEIQIFESR
                     LPNNKIGLKVQVNEGKKVSIKQITFVGNSYFEDKTLQKKMKTKPASFLRSGHFEQEKF
                     DADLQALTAFYKKNGFIDVVIGPYEIQPLGEKYIEIVINVYEGTKYNFGGITIEGNDF
                     FSSEELQDIFTMKIGEPFDQEFFDNEMRKIYTKYFDEGFIYVSIIPNYVKEGDQLIVN
                     LKINENNRARIHQIHITGNRKTKEKVIRRQLEVSPGDYFRQTQVMRSQQNIYNLGFFE
                     QDIRLDYTPINKDGDIDLQLDVIDRASGTANGGVGYNSQDKFVGQLSVSQNNLFGNNW
                     STSLTWEFGGNTQNFEFAFTNPNLLDTDILLGSNLYYTKKTWSSFYYEIYTRGAGIRV
                     GQTIPWIDKTRAVAGYSLYSKKYRITNMNAIMADSTANANLIELSKLNWRYTSVFSLT
                     LSRDTRDNVFFPTKGSQFTLYSEVAGGMFGGDFDYFKEIAQVNWYMDTWEKLILRTKW
                     RFCYVTPYGRSKDAPPDEKFYLGGTGADGIRGFPDSSIGPAGGGTRAIIFSTELGYPL
                     GTDQIIAVAFFDAGNSYNKMRDFNFLNFKKGTGLGIRIRSPFGLIGFDYAYNINDGGW
                     EPHLQFGTTF"
     gene            2515..3465
                     /locus_tag="PLW08_04730"
     CDS             2515..3465
                     /locus_tag="PLW08_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL51942.1"
                     /translation="MIKYKHLFGPVMSRRLGISLGIDLVPYKYCPLNCVYCEVQRTTH
                     QVTKREAFFEMREILAELDSFMSTNPHLDYITFSGAGEPTLNSLLGQIVNYIKAKYPS
                     YKLALLTNGILFSDPEVRKEVLPCDIVLPSLDSATQEGFEKINRPCPEMKVEELIEGL
                     IHFRQEYKGIVWLEVFLVSGINTIPQEISALAKAINNIKPDLVQLNCLDRPGAEDWVK
                     PIPLHTLKQVKEQLSQQVCVPVEIIAKVKYQPAESQLDSELIELLHNTLKRRPSTAED
                     LSAMLDIHINEISKVLSQLYQEKKITAQRESRGVFYKWIS"
     gene            3453..4157
                     /gene="ispD"
                     /locus_tag="PLW08_04735"
     CDS             3453..4157
                     /gene="ispD"
                     /locus_tag="PLW08_04735"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPL51943.1"
                     /translation="MDFLNNTTAIITAAGSGKRLPGNRKKQFRELEGIPILIRSMEPF
                     MASELIDNLVITVPESDIAHTEALIEQWFEGINKPYIVIAGGLERQDSVFSALQVCPE
                     GTEYVAIHDGVRPFVSQELLEILFSAVVIDKAVIPAGKIKHTVMQVEGNYAVNTLPRH
                     QLINVFTPQVFAYSIIMEAYHQAYKDSFFSTDDASLVQHLGMKIRYIWDNDFNIKVTD
                     EADLFFAKQLIEKKKL"
     gene            4169..6913
                     /locus_tag="PLW08_04740"
     CDS             4169..6913
                     /locus_tag="PLW08_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPL51944.1"
                     /translation="MPKFLYLWLKRILCKFGNRELHIPLPVFGLGCGVLGLALPKVSL
                     KMGEYASKLLKALEYRGYDSTGAAFQGNTTEITLLKDVGAPSTLVKTLGIEKQSGKIF
                     CGQVRWATFGFVDKKNAQPHEVKCKRHIYGAHNGNITNTRELKSFLTKEGHFVQSDND
                     GEMLVHTVEHYFDIEMDKAGNPTDIDLRKNCMRKAIIQTADKLVGSYAAVIVDPVTET
                     CWAIKAGSSLYFGIGTLEDMPFALASSDLTAVLRFTKQLVNLREGEFIEYNADNYQVY
                     AQKNLKFKRLNQPDEIWYTGDKIPAKPIYSKLRAEDVELLPEYEYFMEQEIYAQSETT
                     GKLIKLFQGGSNSGKRVLSLLAKAGVRDILLEQVQLFLTENNFAERHRIFTELLNSET
                     FNHFFNQVFSTYREFFDVAVKEDFEKKYFFSNEKNFFLEMVNGEYDLKKISLAKTMDA
                     LAEEMNVKDFNASVDDFLQLVKNTILNSRNAYSIACGTSFHATKIAALFFNSIAGMEI
                     IPILPGDFRGEYSNCIKDNDLIIGVSQSGETKDLIDIFNDIDAKDLNVRKVVLVNNMN
                     STLGQEKSDVAIPILCGPEIAVPATKSFMNQITLFYYLAIKTAQMKLEEIDNNLTQEQ
                     RIRRQQELDDYFASLAKIPSLLKETLDNVSGEIEFMAGKIYMEPSIHILATKISGVAM
                     EGALKIRETVLTHAEGREASEFKHGPNTILGKNTVFGVKHLRSLLRYFSENIDEIESL
                     CEQEGIPHSETKEVYKALADYIFTHNQPFNLSPQGTNIFNRFVQDKDFFEHLYRNYPL
                     IYVTGPDERDVNLTISQINTHKIRGANTFVIAEENEQLLKNASTRPSENSYYAWAYIM
                     LPKTGSSLLTCFSASIVLQLLALKMSVRKMKKLDKLGVLDHGVHPDVPKNVSKSITVD
                     "
     gene            6918..7394
                     /gene="ispF"
                     /locus_tag="PLW08_04745"
     CDS             6918..7394
                     /gene="ispF"
                     /locus_tag="PLW08_04745"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPL51945.1"
                     /translation="MFRCGFGYDVHRLVSDRELILGGVKIPYSKGLSGHSDADVLIHS
                     IIDALLGALALGDIGTFYPDNDLAYKDIDSRILLRKTYTLIREKGWKLNNLDAVICCQ
                     EPKLQPFINTMRANLALDLHCAIEQISLKATTEEGLGISGKGEGISAFCQLLLVKD"
     gene            7397..8146
                     /locus_tag="PLW08_04750"
     CDS             7397..8146
                     /locus_tag="PLW08_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPL51946.1"
                     /translation="MRALGIIGYHQTGKTTLATCLIRELTKKGYNVCSIKDIHSETYR
                     SDTEGKNTFLHTQAGSKAVFAKGLYDSALLFPRPLNLKEMVSYLQADYLIIEGLQNAP
                     VPKIVCAENTDQLEELIDDTCLGISGVISSKMKSFKGLPVFSLPDDLAVLISTIEEKC
                     FDILPLAEPECCSACGKDCYTMAEDIIQGRAKRSDCVLAEQAELKLWVGDREIVIVPF
                     VQKLLQDIVIAFVNNLKDIDPKGNIKLEIKR"
     gene            8143..8760
                     /gene="thiF"
                     /locus_tag="PLW08_04755"
     CDS             8143..8760
                     /gene="thiF"
                     /locus_tag="PLW08_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPL51947.1"
                     /translation="MISREEYFSKRDPSAFPLWQKATVGIAGAGGLGSNIAFSLARAG
                     IGTLIIADYDIVTLENLNRQQYTIAQIGKLKVEALAENIQSFNPFINLVLHPVKITPA
                     NFNAIFGTADLLLEALDEAEQKEMLISCWTSNYPNKHLIVASGLAGYGKNDSIHTEHY
                     GFLHLVGDMKSELKAEISPVATRVAVVANMQANLALELLAETKGM"
     gene            8764..8976
                     /gene="thiS"
                     /locus_tag="PLW08_04760"
     CDS             8764..8976
                     /gene="thiS"
                     /locus_tag="PLW08_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPL51948.1"
                     /translation="MTDKPVFFVNGNKLPWKEGLVVADALKMMNYTFKMLVIKMNGEL
                     IKRENWQTTPIPENADIKVIHLISGG"
     gene            complement(9586..10569)
                     /locus_tag="PLW08_04765"
     CDS             complement(9586..10569)
                     /locus_tag="PLW08_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51949.1"
                     /translation="MKSVYGLLLMGVILVFFSPTLLSDTVKLTNGTVIYGHITREDID
                     KVTIVDQNGIENIIPRNLIIFIDKQLETTREVQKVIITLKSGIEVKGTLILDDANSVT
                     IKDESGIEKKIAKSNIRDIVIDLDKSDERPITIKSYEEPETEIEAETQIVTTPAEKTY
                     NFDFGLKFGSQKPNSNAISDKYSNGIVYGGELIFWLKNKYGIGLNVEKFSEKSEYWFY
                     NEGNYEYNISIIPITLTVYYKHYNNKIVPYIGLGIGQYNLTEKIKYPWYYYYKELEVS
                     DEEYGFHCLFGLQFKCFFAEVKYSTMFKSTWGVEEEYDDIGGLSIAGGLRF"
     gene            complement(10804..11433)
                     /locus_tag="PLW08_04770"
     CDS             complement(10804..11433)
                     /locus_tag="PLW08_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL51950.1"
                     /translation="MEKHLNVTEIFYSLQGESTYSGYPCIFIRLSECNLRCVYCDTQY
                     AFGKGKSMAISSIMEEVKKYPCSLIEITGGEPLLQEDVDALFEELYKFPYKILLETNG
                     AISLEKVPDYVIKIVDIKTPGSGMEKSFLLENLQYLNPQDELKFVITDKVDYQFAVDF
                     LKKYKPQVRCVHFSPVTELLNPKELACWMLEDGLEARLTLQLHKIIGIA"
     gene            complement(11554..12765)
                     /locus_tag="PLW08_04775"
     CDS             complement(11554..12765)
                     /locus_tag="PLW08_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPL51951.1"
                     /translation="MIVKPAISVISPDKMELGIDLYLWGSPFEEIDLRKDCSNAIAYI
                     PLINSHDHLISNWVPRAGNNRPYKNSHIWVEDMKESFAFQERDIFWHNNGSFRLEEPN
                     SLILAKLGAYKSLFCGCSVVQDHSSNQVDAYYEAMPIVVPKNFRQCHSITLGNWWGGE
                     TPEKEMELTKGKMPFIVHLGEGKDEITKGEFSQLKKMGLLRANTLIIHGIALTAEEIS
                     EVANVGASICWCPSSNFFLIGETLKIDEVLKHKANVCIGTDSTMSGGVNLIAEFLKIR
                     EHFPKIPTKEIYRMATVNAATALYLPQYFAFIIPNKCRNLLLVDKVDRDPYENLFALE
                     ASTIQLLLVEGIPRFGDSEWMEILNLDENMYSTFRTGNKEKFVIGDPMELNDQIDEVL
                     GYHKDFPYLPF"
     gene            complement(12762..13952)
                     /locus_tag="PLW08_04780"
     CDS             complement(12762..13952)
                     /locus_tag="PLW08_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPL51952.1"
                     /translation="MIEVLIEKCVGCGACLRACAYDAIKIEEKLAIIDSDKCVLCGAC
                     VSACPFDAILLRKAKTEAIDKSEYNGIWVFAEQREGVIAPVVFELLGKGRELADELGS
                     ELSAVLLGHNIEGLSSELIAFGADQVIEIDDPALKHFWDEGYAYALAELALKYKPSII
                     LAGATVIGRSFIPRVAIRLHTGLTADCTGLSIDPETGDLLQTRPAFGGNIMATIKTAN
                     HRPQMATVRHKVMNPIPRDDTRSGIVIQEQINFDLEEKRTTWLGFEKEKTGLINITEA
                     NIIVSGGRGLKEAKNFALIEELAEALGGAVGASRAAVDAEWIAYSHQVGQTGKTVKPL
                     IYIAVGISGAIQHLAGMSSSDYIIAINKDPDAPIFKVADLGIVGDLFDIVPKLTKRIK
                     EQRV"
     gene            14287..15501
                     /locus_tag="PLW08_04785"
     CDS             14287..15501
                     /locus_tag="PLW08_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL51953.1"
                     /translation="MSENNNKQERKNIFRKLIHFFALNSSMLGMLIMVILVGMGEKMA
                     ERFLPLYLIAMGGSSIVIGLLNGMDNLLSALYSFPGGWLADRIGYKKALKIFNLMAML
                     GYLIVVIFPSWIAVLVGAVFFISWTAISLPATMSLINSVVPANRRSMGISLNSIVKRI
                     PMSLGPILGGALILHYGRIQGVRYAFIVAFILAGVALFIQDKFIQEQPPEAKTERKKG
                     RGLFSANLRNLLVSDILVRFCEQIPYAFVVVWVVENNHINELQFGILTTIEMVTAMLI
                     YIPVAYLADRSTRKPYVAITFVNFTIFPLVLLFSHSFPAMVIAFIIRGLKEFGEPTRK
                     ALIMDLAPENAKAYTFGVYYLIRDVIVAFAAFGGAFLWQKSPQLNFLTASAFGLLGTL
                     YFIFRVKPQKQF"
     gene            15736..16473
                     /locus_tag="PLW08_04790"
     CDS             15736..16473
                     /locus_tag="PLW08_04790"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPL51954.1"
                     /translation="MPIQEEFEKSGAWIFKWRSYLPLLMFILGIGAILDLKNSQFIKY
                     DTIIEVICFSISGCGIAIRVLTIGHTPSGTSGRNTKSQVADKLNTTGMYSLLRHPLYF
                     GNFFIILGVVLFTRHIWVIITYPLIFTLYYERVIFAEEAFLRKKYGEEFVSWASKTPV
                     YFPKFSHYKKPEFPFSWKKVIRNEYQTFFALICSLFALEIISDLIIRQRFVFDPAWQI
                     IFITSCFFYLIIRFINKKTKLLHTSGR"
     gene            complement(16772..17830)
                     /locus_tag="PLW08_04795"
     CDS             complement(16772..17830)
                     /locus_tag="PLW08_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPL51955.1"
                     /translation="MDSAKELRWIRILLLIIALAVVASILKTLKSIFIPLIFSIFLLF
                     LFAPLINYLKKHKVPMVLILLITLVIIAVFLGLVILLIYAASNSLINGLPRYEDKFNE
                     LIAKGTEYLQNLASNWNINTENISIANIAQIISSGFISIPQFISNTVNTLVSIIQNIF
                     LIIFFLIFLLLEIDKLPLRLRKATSKLSKEQTLDILQNIEKQIQNYLTIRTLVNLSAA
                     LLCMLWMLIFGVDFILVCGILLFVLDFIPDVGSVISSAIPILIYLLQSGFSFLWLIFA
                     LLIVATQMLIGNIIEPKLQGVQLNLTPIMVLVSLIFWGWLWGIVGMLICVPLTSAINI
                     ILKQVAPNNFISALISSE"
     gene            complement(17842..18519)
                     /locus_tag="PLW08_04800"
     CDS             complement(17842..18519)
                     /locus_tag="PLW08_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51956.1"
                     /translation="MKISAIFYAIVILAFFMPFFMVSCDQTEVASLSGVKLVIGGEVK
                     LSIAEEIEKIEDVGKKYQNPVVSIQPFALIALFIAVLALVLVLVLPIQYYLLPAYISI
                     IGVLSLQLLNVGILQIITAYQPLFDPSIDLNSILIIKAQPAFWLANLAFILGGIFTLI
                     AGVKSSSISAPAQEPEEVQEEYITSEIQEWVEEYPSEDTFSDSDEENLPAEEENSVLI
                     EEKNTPE"
BASE COUNT         5940 a         3417 c         3582 g         5921 t
ORIGIN      
        1 ttatgccaag aagcattagt tacgcaatac ggactcattg ctaatgccat ccgtaatggg
       61 taagcaatgg gtaagcaatg caattaggga agaacagaaa aaaggggata atagcttgta
      121 ataaaaacag atataaagct aaaccttaaa tatgtgtttt taaaaatgcc ctaaaggaat
      181 tagcaggata aaacaaaaaa atgtctttac taaaaagggc tatttacaat tctggctatt
      241 aactcttgag tggaggaata atgcgaagaa taatactatg cagcatctta attttgctgc
      301 ttggctgtct tggccaaaac ctgtatgctg ttggagaaac catatatgaa ataaaagtaa
      361 tcggagctgt aaatatagat ccggaactgg ttacttccgc actcagtttt agagttggag
      421 atactcttga tcctgaagcc gtggcaaaat ccattcgtaa tttgtataga atgggaatat
      481 tttctgatat tcaagtggaa tctgaacctt atagaacagg agtaactata ctaataaaaa
      541 tccaggaaaa tccgattgta agcagtattg aatatattgg tttcaaagcg gtaaagcagg
      601 aaagaataga tgaactggta aatgttaagg ttggcagtta ctggagtgaa ggagttaaaa
      661 atcagttact aaacaagctg aaaaatgaat atgccaccaa gggtttcagt aatgccgaaa
      721 ttcagatatt tgaatcccgt ttacctaaca acaagattgg tttgaaggtt caggtaaatg
      781 aagggaaaaa ggtctctatc aagcaaataa ccttcgtagg gaatagctat tttgaagata
      841 agaccttgca gaaaaagatg aaaaccaaac ctgccagttt ccttcgctca ggtcattttg
      901 agcaggaaaa atttgatgct gatctgcaag cactcaccgc tttttataag aaaaacggat
      961 ttatagatgt agtgataggt ccctacgaaa ttcaaccgct gggggagaaa tacattgaaa
     1021 ttgttattaa tgtttatgaa ggaaccaaat ataatttcgg gggtataact attgaaggga
     1081 atgatttttt ctcttctgaa gaactgcaag atattttcac gatgaaaatt ggcgaaccct
     1141 ttgaccagga gttctttgat aatgaaatgc gaaaaatcta taccaaatac tttgatgaag
     1201 ggtttatcta tgtgtctatt atcccgaatt atgttaaaga aggcgaccag ctaatcgtaa
     1261 acctgaaaat caatgaaaat aaccgtgccc gaattcacca gatacatatt acaggtaaca
     1321 gaaaaaccaa ggaaaaagtg atccggcgtc agctggaagt ttcacctggt gactattttc
     1381 gtcagaccca agtaatgcga agtcaacaaa acatttacaa tttgggtttc tttgaacagg
     1441 atattcgttt ggattatact cccatcaata aagacgggga catagattta cagcttgatg
     1501 ttatagaccg tgcaagtgga actgctaatg gaggagtggg ttataattcc caggataaat
     1561 ttgttgggca actatcagtg tcccaaaata atctattcgg gaataactgg tctaccagtt
     1621 taacctggga atttggaggc aacacccaaa actttgaatt tgctttcacc aatcctaatt
     1681 tgctggatac cgatattctt ttgggaagca atctctatta cacgaaaaag acctggagct
     1741 ctttctatta cgagatttat actcgtggtg caggaatcag ggttggtcaa accattccct
     1801 ggatagataa aacgagagct gtagccggtt attctctcta ttccaagaaa tacagaataa
     1861 ccaatatgaa tgctattatg gcagattcca ctgctaatgc caacttgatt gaactgagta
     1921 aattgaattg gagatacacc agtgtgttca gtttaacgct aagcagagac acccgggaca
     1981 atgtgttttt ccctactaaa ggaagtcagt ttactcttta cagtgaagtt gcaggtggta
     2041 tgtttggcgg tgattttgat tatttcaagg aaattgctca agttaactgg tatatggata
     2101 cctgggagaa acttatttta cgcaccaaat ggcgtttttg ttatgttact ccttatggca
     2161 ggtctaaaga tgctcctcct gatgaaaaat tctatttggg cggaacaggt gcagatggaa
     2221 taagaggttt tcccgatagt tccattggtc ctgccggagg cggaacaaga gcaattattt
     2281 tttctacgga attaggttat cccttaggaa ccgatcaaat aattgcagtt gccttttttg
     2341 atgccggaaa cagctataat aagatgcgtg atttcaattt cctcaatttt aaaaaaggaa
     2401 ccggactcgg aatcagaatc cgcagtcctt tcggtttaat cggttttgat tatgcctata
     2461 acatcaacga tggcggttgg gaaccccatt tacaatttgg aacaaccttt taagatgatt
     2521 aagtataaac acctttttgg acctgtaatg tcccgtcggt taggtatatc tttaggtata
     2581 gaccttgttc cgtataaata ttgtccctta aattgcgtgt attgtgaagt gcaaagaact
     2641 acacatcagg taactaaacg ggaagctttt tttgagatga gggaaatttt ggctgaattg
     2701 gatagtttta tgtctaccaa ccctcatttg gattatatca ctttttccgg agccggtgaa
     2761 cctacattaa acagtttatt agggcagata gtaaactata taaaagctaa atacccaagc
     2821 tataaacttg ccttgcttac gaatggcata ctgttttccg atccggaagt ccgtaaagaa
     2881 gttcttccct gtgatattgt tttgccgtca cttgattctg ctactcaaga gggttttgag
     2941 aaaatcaatc gtccctgtcc ggaaatgaaa gtggaagagc ttattgaggg tttaattcat
     3001 ttcagacagg aatataaggg aattgtttgg ctggaagttt ttctggtttc aggaattaac
     3061 accattccgc aagaaatttc cgctctggca aaagctatca ataatattaa gccggatttg
     3121 gttcagttaa attgtttaga ccgtcccgga gcggaagatt gggttaagcc catacctttg
     3181 catacactta agcaggttaa agagcagctt tctcaacagg tttgtgtacc cgtggaaatt
     3241 attgccaaag ttaaatatca acctgcggaa agtcaactgg attccgaact tattgaactt
     3301 ttgcataata cgcttaaaag gcgtccttct accgcagaag acctttctgc tatgctggat
     3361 attcacatta atgaaatcag caaagtgcta agtcaattgt atcaggaaaa gaaaataact
     3421 gcccaaagag agagtcgggg ggttttttat aaatggattt cttaaataat acaaccgcaa
     3481 ttattaccgc agcaggaagt ggtaaacgcc taccgggaaa cagaaaaaaa caatttaggg
     3541 aactggaagg aattcctatt ttgattagaa gtatggagcc ctttatggct tcggaactaa
     3601 ttgataacct tgtaattact gttccggaaa gcgatattgc ccacacagaa gcccttatag
     3661 aacaatggtt tgaaggaata aataagccct atatcgtaat tgctggaggt ttggagaggc
     3721 aggattctgt tttttccgct ttacaggttt gtcctgaagg aacagaatat gtagctattc
     3781 atgatggagt gcgtcctttt gtttcgcagg aattgctgga aattcttttt tcggcagtgg
     3841 ttattgataa agcagttatt cctgctggga aaataaaaca taccgtgatg caagtagaag
     3901 gaaattatgc agttaatact cttccgcggc atcaactcat caatgttttc actcctcagg
     3961 tttttgccta ttctataatt atggaagcat atcatcaggc atataaagac agtttttttt
     4021 ctaccgacga tgcttccctg gttcagcatt tgggaatgaa aattcgctat atttgggata
     4081 acgatttcaa tatcaaagta accgatgaag cagacctttt ctttgccaag cagttaatag
     4141 aaaaaaagaa attatagagg acataacaat gcctaagttt ttgtatttat ggttaaaacg
     4201 catattatgc aaattcggaa acagggaact gcatattcct ttacctgttt tcgggttggg
     4261 atgcggtgtt ttaggtttgg cattacctaa ggtctcttta aaaatgggtg aatatgcctc
     4321 caaactgtta aaagctttgg aatatagagg ttatgacagc acgggagccg cttttcaggg
     4381 aaatacaacc gaaataacac tgttaaaaga tgttggcgct cccagcactt tagtaaagac
     4441 cttaggcata gaaaaacaga gcggtaaaat attttgcgga caggtgcgtt gggctacttt
     4501 tggctttgtg gataagaaaa atgctcagcc ccacgaagtt aaatgtaaaa ggcatattta
     4561 tggtgcacat aatggtaata taactaatac cagagagtta aagtcctttt taacgaaaga
     4621 aggtcacttt gtgcaaagcg ataacgatgg tgaaatgtta gtgcatactg tagaacatta
     4681 ttttgacata gaaatggata aagcaggaaa tcctacggac attgatttac gtaagaattg
     4741 tatgcgtaag gcaataattc agaccgcaga taaacttgtg ggaagctatg ccgctgtaat
     4801 tgttgatcct gttacggaga cctgttgggc tattaaagca ggcagcagtt tatattttgg
     4861 catcgggact ttggaagata tgccttttgc gttggcatct tccgatttaa cagctgtttt
     4921 gcgttttact aagcaactgg taaatttgcg tgagggtgaa tttattgaat ataatgcaga
     4981 taactatcag gtttatgcac agaaaaactt aaaattcaag cgtttaaatc aacccgatga
     5041 aatatggtat acaggtgata aaattcctgc caagccaatt tattccaaac tgcgagctga
     5101 agatgttgaa cttttgcctg aatatgaata ttttatggag caggaaattt atgctcaaag
     5161 tgaaactacg ggcaaactga ttaagctctt tcagggcggt tccaatagcg gaaaaagagt
     5221 gttatcatta ttggcaaaag caggagtgcg ggatattcta ttagaacagg ttcagctgtt
     5281 tttaacggaa aacaattttg cagaacggca caggattttt accgaacttc taaattccga
     5341 aacctttaat cattttttca atcaggtttt cagcacttat cgggagtttt ttgatgttgc
     5401 cgtgaaggag gattttgaaa agaaatactt cttttccaac gaaaagaact tctttctgga
     5461 aatggtgaat ggagaatatg atttgaagaa aatctcgctg gctaaaacta tggatgcttt
     5521 ggctgaagaa atgaatgtga aggactttaa tgccagcgta gacgactttt tacagctggt
     5581 taaaaacact attttaaaca gccgcaatgc ctattctatt gcctgtggaa ccagtttcca
     5641 tgccactaag atcgctgctc ttttctttaa ttctattgct ggtatggaaa taattcccat
     5701 tttacctggc gattttagag gtgaatattc caactgcata aaagataatg accttattat
     5761 aggcgtttct caatcgggtg aaaccaaaga cctgatagat attttcaacg atatagatgc
     5821 caaggactta aatgtccgca aagtggtttt ggtgaataat atgaattcca ctttgggcca
     5881 ggaaaaaagt gatgtggcaa ttcctatttt atgcggaccg gaaattgccg ttcctgccac
     5941 caagagcttt atgaatcaaa ttactctgtt ctattatctt gccattaaaa cagcccagat
     6001 gaaattggag gaaatagata ataatcttac tcaggaacag cgtatacggc gtcaacagga
     6061 acttgatgat tactttgctt cccttgccaa aattccttcc cttttgaaag agaccctgga
     6121 taatgtgagt ggtgaaattg aatttatggc tggtaagatt tatatggaac cctccattca
     6181 tattttagct accaaaatca gtggcgtggc tatggaaggt gctttaaaaa taagagagac
     6241 agttttaacg catgccgaag gaagagaggc atccgaattc aaacatggtc ccaatacaat
     6301 tttagggaaa aacaccgttt ttggcgttaa gcatctgcgt tccctcttgc gttattttag
     6361 tgaaaacatt gatgaaatag agtcgctttg cgaacaagaa ggcattccgc acagcgaaac
     6421 aaaggaagtt tataaagcat tggctgatta tatttttact cataatcagc ccttcaacct
     6481 ttcacctcaa gggactaata ttttcaaccg gtttgttcag gataaggatt tctttgaaca
     6541 tctctatcgt aattatccat taatttatgt tacaggaccg gatgagcgcg atgtaaatct
     6601 caccatctct cagataaata cccataaaat acggggtgcc aatacttttg tgattgctga
     6661 agaaaatgag caactgctga aaaatgcctc tacacgaccc agtgaaaaca gctattatgc
     6721 ctgggcttat attatgttgc ccaaaacagg ttcctctcta ttaacctgtt tttccgcaag
     6781 tattgttctg caattgctgg ctctgaaaat gagcgtgcgg aaaatgaaaa aactggataa
     6841 attgggagtt cttgatcatg gtgttcatcc cgatgttcct aaaaatgtat ccaaaagtat
     6901 aacagtggat taagtaaatg ttccgatgcg gttttggcta cgatgttcat cgccttgttt
     6961 ccgataggga acttattttg ggaggggtaa aaattcctta ttccaagggg ctttcgggtc
     7021 attccgatgc cgatgtgtta attcacagca ttattgatgc tcttttagga gctttggctt
     7081 taggggatat cgggacattt tatcccgata acgatttggc ttataaggat attgattccc
     7141 gcattttact gcgtaaaaca tatactttaa tcagggaaaa gggctggaaa ttaaataatc
     7201 tggatgccgt tatctgctgt caagaaccaa aactccaacc ctttataaat acaatgcggg
     7261 ctaatcttgc cttggatttg cattgtgcca tagagcaaat ttcccttaaa gctacaacag
     7321 aagaaggact tggcatcagt ggaaaaggag aaggaattag cgctttttgt caattactgc
     7381 ttgtaaagga ttaaatatga gggcattagg aattattggc taccatcaga ccggcaaaac
     7441 aactttggca acctgcctga taagagaact gacaaaaaag ggatataatg tttgctctat
     7501 caaagacata catagtgaaa catatcgttc cgacactgaa ggtaaaaata cttttctcca
     7561 tactcaggca ggaagtaaag ctgttttcgc taagggctta tatgacagtg ccttattgtt
     7621 tccaagaccg ctgaatttaa aagaaatggt ttcctacctg caagcggatt atctaatcat
     7681 tgaaggtctg caaaatgctc ctgtgcctaa aattgtttgt gcggaaaata cggatcaact
     7741 ggaggaattg attgatgaca cctgccttgg aatatccggg gttatttctt ctaaaatgaa
     7801 gagttttaag ggtttaccag ttttttcctt accggatgat ttggctgttt taataagcac
     7861 gattgaagaa aaatgctttg atattttgcc tttagcagag ccggaatgct gttctgcctg
     7921 tggaaaggac tgttatacta tggcggaaga tattattcag ggcagggcaa aaaggagtga
     7981 ttgcgttttg gctgaacaag cagaactcaa actttgggtg ggggataggg aaattgtgat
     8041 tgtgcctttt gtgcaaaaat tattacagga tatcgttatt gcttttgtga ataaccttaa
     8101 agatatagac cccaaaggca atattaaatt ggagataaag cgttgatttc ccgggaagag
     8161 tatttttcca agcgcgatcc ttccgccttt cctctttggc aaaaagcaac tgtaggaatt
     8221 gctggagcgg gaggtttggg ttcaaatatt gccttttcct tagccagagc tggaattgga
     8281 actttgatta ttgccgatta cgacattgtt acgctggaaa atcttaatcg ccagcaatat
     8341 actatagccc aaattggcaa attgaaagtg gaggcattag cggaaaatat tcagtccttt
     8401 aatcccttta ttaatcttgt tttgcatccg gtaaaaatta ctcctgccaa ttttaacgct
     8461 atttttggaa ctgcagacct gcttttagag gcgttggatg aggcagaaca aaaagaaatg
     8521 ctaatttcct gctggacatc taattatccg aataaacatt taattgtggc ttcaggattg
     8581 gctggctacg gtaaaaacga tagcatacat acggaacatt acggtttttt gcatcttgta
     8641 ggcgatatga agagtgaatt aaaggcggaa ataagccctg ttgcaacccg tgttgcagtt
     8701 gtagccaata tgcaggcaaa tttggctttg gaactacttg cggaaacaaa agggatgtaa
     8761 aaaatgacag ataaacctgt tttctttgta aacggaaata aattaccctg gaaagaagga
     8821 ctcgttgttg cagatgcttt gaaaatgatg aattacacct tcaaaatgct ggttattaaa
     8881 atgaacggtg aacttatcaa aagagaaaac tggcagacca ctcccattcc agaaaatgcc
     8941 gatatcaaag ttattcatct gatcagcggt ggttgatgaa aaatcaaggg tatttttccc
     9001 gcagattacg catatttcac gcagatttcg cggaatttat ttataattga actaaaattc
     9061 agccatttat cataaaccca actaaggaag tagaagtaat ggttaatatt atttccagat
     9121 ttattaatca tatattgtag aaaaagttcc gttggaacgc cagatattaa caacgggttt
     9181 tgaacccggt gataggtaag caaaagttat ttttattatt agctggattc ctgctttcgc
     9241 atgaatgaca ttagaacgcc agatattata gaagggtttt aaccctgaga aaaaaagtca
     9301 aattcggttt atgggttgct gaattccgat tcgccataaa tagaggacat ttgattttca
     9361 gagttctgtg aactcttgtg caaagacaaa atttgccaaa ccatttttcc tcaactgcat
     9421 tattggggtt tatattattt gcttctttcg gaaaagttcc ttgcttactt cccataaaca
     9481 actcatcatc cacccactct tgaggttggg ttgaagttgg gaagaaatta atgacgggga
     9541 catcgttatt ccggaggaaa tagtaatttt tcggaacttt gttaattaaa acctgagccc
     9601 gcctgcaata ctcaagcccc caatatcatc atattcttct tcaactcccc aggtactttt
     9661 aaacatcgtg gagtatttta cttcagcgaa gaaacactta aattgtaaac caaataaaca
     9721 gtgaaagcca tattcttcat ctgatacctc taattcttta taatagtaat accagggata
     9781 tttaattttt tcagttagat tatattgtcc aatacctaaa ccaatataag gcactatttt
     9841 attattataa tgcttataat aaactgttag agtaatagga ataatagaaa tattatattc
     9901 ataattaccc tcattataga accaatattc tgatttttca ctgaattttt caacatttag
     9961 tccgattccg tatttgtttt tcagccaaaa aatcaattca cctccatata caattccatt
    10021 actgtattta tcagaaatag catttgaatt gggtttttga gagccgaatt ttagtccaaa
    10081 atcaaaatta taggtttttt ccgcaggagt ggtaacaatt tgcgtttccg cttctatttc
    10141 tgtttctggt tcttcataac ttttaatagt aattggtctt tcgtccgatt tatctaaatc
    10201 aataacaata tcgcgaatat tgctttttgc tattttcttc tcaattcccg attcatcttt
    10261 gatagttacg ctgttagcat cgtccaggat taaagttcct ttaacctcaa ttccgctctt
    10321 taaagtaata attactttct gaacctctct tgttgtctct aattgtttat ctatgaagat
    10381 gataaggtta cgtggaatga tgttttcaat tccattttga tctacaattg taactttgtc
    10441 tatgtcttct ctggtgatat gtccataaat tacagttccg ttagtcaatt taacggtatc
    10501 ggatagcaaa gtaggactaa aaaaaaccag aatgacaccc attaaaagta agccataaac
    10561 acttttcata atttgaacct ccattatgta gtcggagctt gctgtagctc cgcaatttgt
    10621 gtagcttgag tttgctggtg ctccgtattt tttttgtgca ggaaaagcat cctctgtcta
    10681 cattgaatac aaaaactttt aatcatcttt tctcatcctt cgggaacata ataggataaa
    10741 gtcaattttt ctctttcctc tttgtaaaaa actatctctt tttactcttt aggcaaggta
    10801 attttatgct atcccgatta ttttatgcaa ttgcaatgtc aatcttgctt caagtccatc
    10861 ttccagcatc cagcaagcca attctttggg gtttaaaagt tctgtaaccg gtgaaaaatg
    10921 aacacaccta acttggggtt tatacttttt taagaaatca acggcaaact gatagtcaac
    10981 tttatcagtt atcacaaact tcagttcgtc ctggggattc agatattgca gattttccag
    11041 aagaaaactc ttttccattc cactgccggg ggtttttata tctacaattt taattacata
    11101 atcaggaact ttttccagag agatagctcc gttggtttcc agcaaaatct tgtaagggaa
    11161 cttatacaat tcctcaaaca aggcatccac atcttcctgg agcaagggtt cgcctccggt
    11221 aatttcaata agggaacatg gatatttctt cacttcttcc attatggaac tgatagccat
    11281 tgattttccc ttaccgaagg catattgggt atcacaataa acacagcgta aattgcattc
    11341 agagagacgg ataaaaatac agggataacc actgtaggta gattctccct gtaaagaata
    11401 gaaaatctct gttacattca agtgtttttc catcttttaa cccgataata ttctctcaca
    11461 gattacacag attacacaga tttcaataaa taagaatttt tttcaaactc ctcattcatt
    11521 ctccatttcc acaagacctc aagacctctc aacctaaaaa ggcaaatacg gaaaatcctt
    11581 atgatacccc aaaacctcat caatctgatc atttaattcc atagggtcac caatcacgaa
    11641 tttctctttg tttccagtgc ggaaggtgct atacatattt tcatctaaat tcaaaatttc
    11701 catccattca ctatctccga aacggggtat tccttctacc aataaaagct gtatggttga
    11761 tgcttccagg gcaaaaaggt tttcataggg atcgcggtca actttatcca ccagcaatag
    11821 attacggcat ttattaggaa taataaaagc aaaatattga ggtagataaa gtgctgttgc
    11881 tgcattaacg gtagccatcc gatagatttc tttggtagga attttgggaa aatgctctct
    11941 gattttaagg aattcagcaa tcagattcac tcctccggac atggtggaat ccgttccaat
    12001 acatacattt gccttatgct taagaacttc atcaatcttt agtgtttctc cgataaggaa
    12061 aaaattggaa gaaggacacc agcaaataga agctccaaca ttggctactt cactaatttc
    12121 ttctgcagtt aaggcaatgc catgaataat taaagtattt gctctcaaca atcccatttt
    12181 tttcagctgg gaaaattccc cttttgtaat ttcgtctttt ccttcaccca agtgaactat
    12241 aaaaggcatt ttccctttag ttaattccat ttctttttcc ggagtttcac ctccccacca
    12301 gttaccgaga gttatagaat gacactgacg gaaattttta ggtacaacaa ttggcattgc
    12361 ttcataatag gcatccacct gattggatga atggtcctga actacactgc atccgcaaaa
    12421 taagctttta taggcaccca gcttagccaa aataagcgaa ttgggctctt ccaaacgaaa
    12481 acttccgtta ttatgccaaa aaatatccct ttcctggaag gcaaaggatt ccttcatatc
    12541 ttcaacccag atatgagaat ttttgtaggg acggttgttt cctgctcggg gaacccagtt
    12601 actgattaaa tggtcatgag aattaattaa gggaatatag gcaatggcat tggaacaatc
    12661 cttgcgcagg tctatttcct caaagggact tccccaaaga tataaatcaa ttcccagctc
    12721 cattttgtca gggctgataa cactgatcgc gggtttaact atcatactcg ttgttctttt
    12781 atcctttttg ttaatttggg aacaatatca aaaagatcac ctacaatgcc taaatccgca
    12841 actttgaaga ttggagcatc aggatctttg ttgatagcga taatataatc ggaactggac
    12901 attcctgcaa ggtgctgaat tgcaccactt attcctactg caatataaat aaggggttta
    12961 actgtttttc ctgtctgtcc aacctgatgc gaataagcaa tccattctgc atcaactgca
    13021 gctctggaag ctccaactgc tcctcctaat gcctcagcca gttcttcaat taaagcaaaa
    13081 ttctttgctt ctttcaagcc ccttccaccg gagacaatga tattagcttc agtgatatta
    13141 attaaaccgg ttttttcctt ttcaaaacca agccaggtag ttcttttctc ctccaaatca
    13201 aaatttatct gttcctgaat tacaattcca ctgcgagtat catctctggg aatgggattc
    13261 ataactttat gacgcactgt agccatttgt ggacgatgat tagctgtttt aattgtagcc
    13321 ataatattgc ccccgaaagc aggacgggtt tgtaataaat ctcctgtttc cggatcaatg
    13381 gaaagtcctg tacaatcagc agttaagcca gtatgtaaac gaattgccac ccgaggaata
    13441 aaacttcttc cgataacagt agctccagca agaataatag agggtttata cttcaaagca
    13501 agttcggcaa gagcataagc ataaccttca tcccaaaaat gttttaatgc aggatcatca
    13561 atttctataa cctgatctgc tccaaaagct ataagctcgg aagaaagacc ttcaatatta
    13621 tgtcctaaaa gaacagccga aagttctgaa ccaagctcat cggcaagttc acgacccttt
    13681 cctaaaagtt caaaaactac aggagcaata acgccttctc tttgttcggc aaaaacccaa
    13741 attccattat attcactttt gtcaattgct tctgttttgg ctttccgtaa taaaatagca
    13801 tcaaaaggac acgctgaaac gcaggcacca cataaaacac atttatcact gtcaataata
    13861 gcaagctttt cttcaatttt tatggcatca taagcacagg cacgcaaaca agcaccgcaa
    13921 ccgacgcatt tttcaattaa cacttcaatc ataaacattc tcctttttcc attaattttt
    13981 atatttattt ttctgtcaag tccaaagtga aagagaagga aaatgcaaat ggagaaggaa
    14041 ttaagtaagg agtagtcact gcctttaaca attttatcat tttttctaac taatttggca
    14101 gtgaggactt gtaacggtat aagttaaaaa tacagtaagc cataaaaaat cagccatatc
    14161 cgcgtgcttt tatttattca gggtttgggg ttttttcaaa gggttaaaaa aatttgactg
    14221 ctgattaata atgccttata ttgttaatat aatgaagatg ttttgcagtt gtcaggagct
    14281 tttttaatgt ccgaaaataa taataagcag gaaagaaaaa acatattccg gaaactaatt
    14341 cattttttcg ctctcaacag cagtatgctg ggaatgttaa ttatggttat tcttgtaggg
    14401 atgggcgaaa aaatggcgga acgctttttg cctttgtatc ttatcgctat gggtggcagt
    14461 tctatagtta ttggtctctt aaatggaatg gacaatcttt taagtgcttt atattctttt
    14521 cctggaggtt ggttagcgga tagaataggt tacaaaaaag cattaaagat ttttaactta
    14581 atggctatgt tgggctattt aatcgtggtc atttttcctt cctggatagc agttttagtg
    14641 ggggctgtat tttttatttc ctggacagct atttctttac cggcaacgat gagtttgatt
    14701 aattccgtag ttcctgccaa cagacgctct atgggaattt ctctgaattc tattgtaaag
    14761 cggattccga tgtctttagg tccgatttta ggtggtgcct taattttaca ttacggacgc
    14821 atccagggcg tgcgttatgc ctttatagtg gcttttattt tagcaggagt ggctttattt
    14881 attcaggata aatttattca agagcaacct ccggaagcga aaaccgaaag aaaaaaaggg
    14941 aggggtcttt tttctgccaa tttgcgtaat ttacttgttt ccgatatcct ggtgcgtttt
    15001 tgtgaacaga ttccttatgc ctttgtagtt gtttgggtag tagaaaataa tcatattaac
    15061 gaattgcagt ttggtatttt aactactatt gaaatggtaa cagcaatgct gatttatatt
    15121 ccggttgctt atctggcaga tagaagcact cgcaaacctt atgtagcaat tacttttgtt
    15181 aattttacta tttttccttt ggttcttctt ttcagtcata gttttcccgc aatggtaatt
    15241 gcttttatta ttcggggctt gaaagaattt ggagaaccga cacgaaaggc attgattatg
    15301 gatttagctc cggaaaatgc taaggcatat acttttggag tttactatct gattcgggat
    15361 gtaattgttg cttttgcagc atttggagga gctttcctgt ggcaaaaaag tccgcaatta
    15421 aatttcttaa ctgcttcggc ttttggcttg ctgggaacgc tgtatttcat ttttagggtt
    15481 aaaccgcaga aacagtttta gccactatga gcaccgaatt cagcaaactt atttttaaca
    15541 tagagaaaag tggaattaaa agcgattatg aaaatgataa ttactgagat gaaaatatgg
    15601 aagatggaat taaattggaa gagaaaatgc ttaaatctat aattgtaatg aaaagggata
    15661 actaccatat attatcactt actttagata agtttatata tatctgtacc aaataaaaca
    15721 aatttaggag tgaaaatgcc cattcaagaa gaatttgaaa aatcaggggc ttggatattc
    15781 aagtggagaa gttatcttcc acttttaatg tttattctcg gtattggagc tattctggac
    15841 ttaaaaaata gccaattcat aaaatacgat acgataatag aagttatctg cttttctata
    15901 agcggttgtg gcatcgcaat tagagtactt actattggtc ataccccttc cggaacttcc
    15961 ggtagaaata ctaaaagtca ggttgccgat aaattaaata ctacaggtat gtattctctt
    16021 ctcaggcatc ccttatattt tggtaatttt ttcattattt tgggggtggt gttatttacg
    16081 aggcatattt gggttatcat aacttatccg ctcattttta ccctttatta tgagcgggtg
    16141 atctttgcgg aagaagcttt tctccgaaag aaatacggag aggaatttgt atcttgggca
    16201 agtaaaacac ctgtgtattt ccctaaattc agtcattata aaaaacccga atttcctttt
    16261 tcctggaaaa aagttatacg caatgaatat cagacatttt ttgctcttat ctgctcactt
    16321 tttgctctgg aaataattag tgatcttatt atcagacaaa gatttgtctt tgatcctgct
    16381 tggcaaatca tttttattac gagctgtttt ttttatctca taatcaggtt tataaataaa
    16441 aagacaaaac tcttgcacac ttccggcaga taaatacagg aagtaattta ttttctcttt
    16501 tggggttatg cccagttgag ctttttatat tcctgcctaa aagctggaga aaacattcta
    16561 tctttctgct atgtaaatac ttagaatgat atgaaacaca tcatcttctc cgtcttcaat
    16621 ttgaacacct aattgtttaa aaattagttc gttccaagtc aaccccgtaa cacttccgta
    16681 acacttccgt aacattgccc ttatctcgtt acggagtcgt tactcaattg ttacataatc
    16741 tttacagggt aaagcaagct taaaaaaaat attattcgct gctgatcagg gcggaaataa
    16801 aattatttgg tgcaacctgt ttcagaataa tattgatagc agaagtcaat ggcacacaaa
    16861 tcagcattcc tacaattccc caaagccaac cccaaaaaat gagagaaacc aataccatta
    16921 tgggagttag atttaactgc acaccctgta acttgggctc aattatattg ccaatcagca
    16981 tttgagtggc aacaatcaat agggcaaata ttaaccataa aaaactgaag ccactctgca
    17041 gcaggtaaat aagtatggga attgcggaag aaataactga acctacatcg ggaataaaat
    17101 ccaagacaaa aagcaaaatt ccgcagacaa gaataaaatc cacgccgaaa ataagcatcc
    17161 acagcataca cagaagcgcg gcagataaat taactaaggt tctgatagtt aaatagttct
    17221 gaatttgttt ttctatgttt tgcaggatat ccaatgtctg ctctttgctt aatttggagg
    17281 tagcttttct taatcgcaat ggcaatttgt caatctccaa aagcaaaaag ataagaaaaa
    17341 agatgattaa aaagatattc tgaatgatac ttacaagagt gttcaccgtg ttgctgataa
    17401 attgaggaat ggaaataaat ccgctgctta taatttgtgc tatattagca atggaaatat
    17461 tctccgtatt gatattccag ttagatgcaa ggttttgaag atattctgtt cctttggcta
    17521 ttagttcatt aaatttatct tcataacgag gcaacccatt gattaaacta ttggaagcag
    17581 cataaattag caatattact aaccctaaaa aaacagctat tatcactaaa gtaatcagca
    17641 agattaagac cattggaact ttatgcttct tcagatagtt aatcagcgga gcaaaaagaa
    17701 aaagcagaaa aatagaaaag attaagggaa taaagatact cttaagcgtt ttgagaatag
    17761 aagccacaac ggctaaagcg atgataagca ataatattct tatccagcgc agctcttttg
    17821 cactatccat atatgctcta tttactcagg tgtatttttt tcttctataa gaacagaatt
    17881 ctcttcttcc gctggcaaat tttcttcatc cgagtcagaa aatgtgtcct ctgaaggata
    17941 ttcctccacc cattcctgaa tttcagaagt aatatattcc tcttgaactt cctccggttc
    18001 ttgtgctggt gccgatatag agctactttt cacacctgca atcagagtaa aaataccccc
    18061 taaaataaaa gccaaatttg ccagccaaaa agccggctgc gctttaataa tcagaatgct
    18121 gttcaaatct atggaaggat caaaaagcgg ctgataagca gttataattt gcagtattcc
    18181 tacatttaac aattggagag aaaggacacc tataatacta atatatgcag gtagtaaata
    18241 gtattggata ggaagaacca aaaccaaaac taatgccaat acagctataa ataatgctat
    18301 taaagcaaag ggctgaatac ttacaacagg attttgatat tttttaccta catcctctat
    18361 tttttctatc tcttctgcta tagaaagttt tacttcacca cctataacta atttgacgcc
    18421 acttaaagac gcaacttctg tctgatcaca actgaccata aaaaacggca taaaaaacgc
    18481 aagaattaca atagcgtaga aaattgctga gatcttcatt ctatcctccc tttcttttac
    18541 tgttttcaga taatataaat cattatatac tgtcaagcaa aatcggtttc ttcctttttt
    18601 gacttacaat tttcacttgc tttccagaaa accaccatcc actaattatt gcactttcaa
    18661 cctgcctttt ttgactcaca attttcactt gccttccaga aaagcactat ccactaatta
    18721 tttcactttc aaccgagcaa taactgaatc tccaattaaa aaattgtgag ttaaaaaagg
    18781 ttccagggtt aaaaccctct ctatgatctg tcgttcctac ggaactttcg tccaaaaaga
    18841 aatacagttt tataggttct
//
LOCUS       DAOOZV010000035        18585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3083_length_18585_cov_56.256989, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000035 DAOOZV010000000
VERSION     DAOOZV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18585
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_18585_cov_56.256989"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..376)
                     /locus_tag="PLW08_04805"
     CDS             complement(194..376)
                     /locus_tag="PLW08_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51957.1"
                     /translation="MNNLSHQILGRRNCQIFFLRDDGMDLFFTKREREKEKKRESEKS
                     NFLQREKEKKKKREKV"
     gene            618..944
                     /gene="rpsJ"
                     /locus_tag="PLW08_04810"
     CDS             618..944
                     /gene="rpsJ"
                     /locus_tag="PLW08_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPL51958.1"
                     /translation="MSKHDNRIRIKLKAYDHRLLDKSVAEIVKSTRNTGAKVVGPIPL
                     PTDRTIYTILRSPHADKKSQDQFQMLVHKRLIDIVNPTQQTTNALKKLSLPAGVHVEI
                     KANTRS"
     gene            947..1579
                     /gene="rplC"
                     /locus_tag="PLW08_04815"
     CDS             947..1579
                     /gene="rplC"
                     /locus_tag="PLW08_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPL51959.1"
                     /translation="MIGLIGKKIGMTQVFDSDGKVIPVTVIKAGPCKVICKRTEAENG
                     YEAIQVGYEEIPARKVSKPLQGHFKKYNSPFYRYVREFRPSYGQHIESVQIGDELKAD
                     IFGENETVSVSANSKGRGFTGVMKRHNFNGFISSHGSHESFRGPGSIGQCAQPSRVFK
                     GMKMAGQYGNTKVTVHHLKVVKVDPENNIIMVKGAVPGHRNSLVTIHKEQ"
     gene            1590..2225
                     /gene="rplD"
                     /locus_tag="PLW08_04820"
     CDS             1590..2225
                     /gene="rplD"
                     /locus_tag="PLW08_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPL51960.1"
                     /translation="MLTAKRYNMQGELLGEVDLPEGIFEVDVNAPKILLNEVVTMFLA
                     NQRQGTVQKKNRSMTAGSTRKLFRQKGTGNARVGTRRSPIRVHGGKAFAIYPKDWYRK
                     IPRTKKRLALKVALTDRAREGRIMIVDNLSFEKPNTKLAVELLNKIIPEKGYKLVVTD
                     GHHIPTVKSFANLPEVMTDRADSLNAYEVLKSSYIIMTDEALKKVEEVFNK"
     gene            2222..2509
                     /gene="rplW"
                     /locus_tag="PLW08_04825"
     CDS             2222..2509
                     /gene="rplW"
                     /locus_tag="PLW08_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPL51961.1"
                     /translation="MIHPRNIIISPIITEKSSNQVQNENTYSFKVSSNANKIEIKKAI
                     ERIFAVKVLAVNTIRYKGKPKSLGKYNGKRPDWKKAIVTLREGDKIADFEV"
     gene            2521..3348
                     /gene="rplB"
                     /locus_tag="PLW08_04830"
     CDS             2521..3348
                     /gene="rplB"
                     /locus_tag="PLW08_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPL51962.1"
                     /translation="MGIKKYRPTTSSLRFRTGHTFEEITKKTPEKSLLKPKHRTGGRN
                     NSGRITCRHRGGGHRRHYRIIDFKRDKIGIPAKVAAIEYDPNRTARIALLHYVDGEKR
                     YIVAPDGLEVGAKVMSGPDADIAVGNAIPLEKIPLGSVVHNVELKRGKGGQIARSAGT
                     FAQVVAKDGDYVHIKMPSNDVHLIRKECLATIGSVSNPDHSLIQIGKAGRKRWMGIRP
                     SVRGVAMNPVDHPMGGGEGKTSGGGHPVSPWGKPAKGGKTRKTHKYSDKYIVKAVKK"
     gene            3365..3643
                     /gene="rpsS"
                     /locus_tag="PLW08_04835"
     CDS             3365..3643
                     /gene="rpsS"
                     /locus_tag="PLW08_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPL51963.1"
                     /translation="MARSIKKGPFVDAHLLKKVEVLNAEGKKSVIKTWSRRSTITPQF
                     IGHTFSIHNGHKFVPVYITENMVGHKLGEFSPTRTYRGHKEKKKKVTR"
     gene            3654..3998
                     /gene="rplV"
                     /locus_tag="PLW08_04840"
     CDS             3654..3998
                     /gene="rplV"
                     /locus_tag="PLW08_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPL51964.1"
                     /translation="MEAKAKLCYARGSARKARLVLDTIRYKPVTEAQNILRFSRRRVA
                     GTINKLLASAIANAQHQDPKVNLDKMYVITATADEGPQMKRYMPRAMGRAYMIRKPTC
                     HISLEIQTIEEE"
     gene            4009..4656
                     /gene="rpsC"
                     /locus_tag="PLW08_04845"
     CDS             4009..4656
                     /gene="rpsC"
                     /locus_tag="PLW08_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPL51965.1"
                     /translation="MGQKIHPVLYRLGVNKETESIWFAQGTSYVDFLQEDIKIRNYIR
                     KRLSDKMVSRVQISRKTSSIIIDIYTARPGLVIGKKGEDIDKLRNELNVFINKDRKNP
                     ISVSINIEPIDKMWLDARLVAQEIARQLEERISFRRAMKMAIRYVMKENAQGVKVQVS
                     GRLGGAEIARSERYKQGRTPLHTLRADIDYANVEANTTYGVIGVKVWIYKGDILD"
     gene            4669..5088
                     /gene="rplP"
                     /locus_tag="PLW08_04850"
     CDS             4669..5088
                     /gene="rplP"
                     /locus_tag="PLW08_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPL51966.1"
                     /translation="MLAPKKVKHRKMMKGRRKGLSWTGCNISFGDYGLIALEDSFITS
                     RQIEACRIAITRHMKREGKVWIRIFPDKPITKKPAETRMGKGKGAPEYWVAVVRPGRV
                     LFEIEGVDVNVAKEALRLAAHKLPIKTRFIAREGVEL"
     gene            5085..5285
                     /gene="rpmC"
                     /locus_tag="PLW08_04855"
     CDS             5085..5285
                     /gene="rpmC"
                     /locus_tag="PLW08_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPL51967.1"
                     /translation="MKIEEIRDLSIEELQAKLEELRIELFNLRFQKTKNLLDRPDRIR
                     NIRHDIARINTILTEREKEKKI"
     gene            5312..5548
                     /gene="rpsQ"
                     /locus_tag="PLW08_04860"
     CDS             5312..5548
                     /gene="rpsQ"
                     /locus_tag="PLW08_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPL51968.1"
                     /translation="MIKQGIVVSDKNDKTIVVRVERQFIHPLYKKTVRRHKKFMAHDE
                     NNSAHEGDIVQIMESRPLSARKRWTLHKIVERSK"
     gene            5564..5932
                     /gene="rplN"
                     /locus_tag="PLW08_04865"
     CDS             5564..5932
                     /gene="rplN"
                     /locus_tag="PLW08_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPL51969.1"
                     /translation="MIQVQTMLNIADNSGAKKAMCIKVLGGTQRKYASIGDVIVVAIK
                     SATPGGKVKKGSVERAVIVRTRKELRRPDGSYIRFADNAAVIIDEKHEPKGTRIFGPV
                     ARELREAHYMKIVSLAPEVL"
     gene            5941..6261
                     /gene="rplX"
                     /locus_tag="PLW08_04870"
     CDS             5941..6261
                     /gene="rplX"
                     /locus_tag="PLW08_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPL51970.1"
                     /translation="MAKNKMRFKKGDLVKVIAGDEKGRKGHILKVFPKTGKVIVEKVH
                     MIKKHAKPTQQNPQGGIITMEAPIDASNVMLFNEKLNAVSKPVYQVREGHRIRVCKKS
                     GDEL"
     gene            6275..6820
                     /gene="rplE"
                     /locus_tag="PLW08_04875"
     CDS             6275..6820
                     /gene="rplE"
                     /locus_tag="PLW08_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPL51971.1"
                     /translation="MNRLKERYKTQVMDNLMKRFGYKNPHQVPRLEKIVVSMGVGQAT
                     QNKAILDNAVKDMETICGRKPVVTKARKSISNFKLRKGMPIGCKVTLRNEVMYEFYDR
                     LISIAIPRIRDFRGIPADSFDGRGNFSFGLKEQTVFPEIEYDKIDMIRGLNITIVTTA
                     KTDEECRALLQELGMPFQKNE"
     gene            6831..7016
                     /locus_tag="PLW08_04880"
     CDS             6831..7016
                     /locus_tag="PLW08_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPL51972.1"
                     /translation="MAKKSLIIKQQRTPKYKVRKYNRCKICGRPRSYMRDFGMCRLCF
                     RKYASEGQIPGITRSSW"
     gene            7035..7448
                     /gene="rpsH"
                     /locus_tag="PLW08_04885"
     CDS             7035..7448
                     /gene="rpsH"
                     /locus_tag="PLW08_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPL51973.1"
                     /translation="MSISDPIADALTKIRNAYRAGHTQVIVNHNKLIEALVNILAEEN
                     FVNSFQVLDKDPEHKINYKRILIILRYTNAGEPVIQGIQRISKPGRRVYVKADKLPSV
                     YNNTGCAVISTSMGLMVDRDARLKNVGGEYICKVW"
     gene            7458..8030
                     /gene="rplF"
                     /locus_tag="PLW08_04890"
     CDS             7458..8030
                     /gene="rplF"
                     /locus_tag="PLW08_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPL51974.1"
                     /translation="MSRIGRAPIKLQPGTQVDVVDNKITVKGKLGELNWSLLPGITVE
                     VEENLLKVKRADDSKNQRAFHGLSRALIQNMITGVTEGFQKILHIYGTGYSAEVVGPW
                     LKLTLGFSHDILLQIPEGLEVTTEAVPRSKGMRSDFQSIITIKGIDKQLVGEFAAEVR
                     GCRPPENYKGKGIRYFDEYVIIKAGKAGTK"
     gene            8046..8417
                     /gene="rplR"
                     /locus_tag="PLW08_04895"
     CDS             8046..8417
                     /gene="rplR"
                     /locus_tag="PLW08_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPL51975.1"
                     /translation="MITSSCKIKRDLRNRRHLSIRKRVSGTPERPRLVVFRSLKNIYG
                     QIIDDTQGVTLVSMSTIAKDIGSLTGKKKTEQSYEVGVKLGEKALAAGITKVTFDRAG
                     YKYHGRVKALAEGARKAGLEF"
     gene            8427..8945
                     /gene="rpsE"
                     /locus_tag="PLW08_04900"
     CDS             8427..8945
                     /gene="rpsE"
                     /locus_tag="PLW08_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPL51976.1"
                     /translation="MNYEQNHPDEEKLIEKIVETKRVAKVVKGGRNFSFTAIVVVGDK
                     QGNVGVGNGKANEIVDAIRKAKEKAVKNMFKVPIVKGTVPHEIVARYGASKVLIKPAA
                     PGTGIIAGGTARAIFEAAGIENILCKSLGSNTPTNVVKATINGLKSMRTLSDISRLRN
                     KTMAQLTGQEEK"
     gene            8942..9127
                     /gene="rpmD"
                     /locus_tag="PLW08_04905"
     CDS             8942..9127
                     /gene="rpmD"
                     /locus_tag="PLW08_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPL51977.1"
                     /translation="MKLKVTQTRSTINRIEDHKKVIKALGLGKIGKSRIHNDTPSLRG
                     MINKVAYLLKVEELKEE"
     gene            9130..9585
                     /gene="rplO"
                     /locus_tag="PLW08_04910"
     CDS             9130..9585
                     /gene="rplO"
                     /locus_tag="PLW08_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPL51978.1"
                     /translation="MLTLSDLERPSCKKNKKRLGKGQGSGWGHQAGRGHKGKKARAGG
                     NVPPRFEGGQMPLNRRVPKRGFKNIFRENYRVLNLYRLQDLDITELDIPTMEKLGLIP
                     GKGKKANAPVKVLAGVKDEFTKTVTIKANAFSQKAKEIIEKNGGKAEVV"
     gene            9608..10900
                     /gene="secY"
                     /locus_tag="PLW08_04915"
     CDS             9608..10900
                     /gene="secY"
                     /locus_tag="PLW08_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPL51979.1"
                     /translation="MFRIPDLKKKILFTALFLVLYRLGSFIPLPGVNATALKAFFEGA
                     REGGNTLFGLLDLFVGGSFERASVFALGIMPYITASIVIQLLGSIIPYFEKLRKEGAD
                     GQKKLNQITRYGTVALAAFNAITITMWLTNLSGGVVPRPNFLFHLTAIITLVTGTMIV
                     MWIGEQITEHGIGNGISLIIFAGIIARYPEGFVRMIQNIPGNPSYVWKAIISVVLMVL
                     VTAAVIFVTEAVRKIPVQYAKRIVGRRVYGGQSTYIPLRVNTAGVIPIIFAQSVLMFP
                     ATIAALLGGDSYFWTTLRNWLSPGAVLYTILYVGLIIFFAYFYTAIVLNPTEMAENMV
                     KYGGHIPGKKPGKKTAEYINSVLVRITLPGAIFFAFIALLPEIMSNRLDLPFYFGGTG
                     LLIVVGVALDTLQQIESHLVMRHYDGFMKKGKLRGRSS"
     gene            10902..11663
                     /gene="map"
                     /locus_tag="PLW08_04920"
     CDS             10902..11663
                     /gene="map"
                     /locus_tag="PLW08_04920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPL51980.1"
                     /translation="MIYIKTPAEIEKMRKSCRIIGELLDALETIIRPGISTLEIDKFA
                     EDFIGSRGGKPSFKGYRVDGLPPFPGSICASVNEQIVHGIPSAKKILQDGDIIGIDVG
                     VVLDGYNGDAARTYKVGKISAEAEELLQVTKEALERGISAAIPGNRVGDISYEIGSFV
                     AFRGYYVADNLTGHGVGRNLHEEPQIPNTGKPGRGPRLSAGMTLAIEPMVNIGTNRVL
                     ENGWEFKVADNSLSAHFEHTILVTDNKPEILTVAD"
     gene            11677..11895
                     /gene="infA"
                     /locus_tag="PLW08_04925"
     CDS             11677..11895
                     /gene="infA"
                     /locus_tag="PLW08_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPL51981.1"
                     /translation="MSKAGVIEVEGVVTEALPNTTFRVTLENGHEILAHSSGKMRMNY
                     IRILPGDKVKVELSPYDLTRGRITYRYK"
     gene            11944..12060
                     /gene="rpmJ"
                     /locus_tag="PLW08_04930"
     CDS             11944..12060
                     /gene="rpmJ"
                     /locus_tag="PLW08_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPL51982.1"
                     /translation="MKVKASVKKICKDCKIIKRNGIIRVICSSNPKHKQRQG"
     gene            12072..12440
                     /gene="rpsM"
                     /locus_tag="PLW08_04935"
     CDS             12072..12440
                     /gene="rpsM"
                     /locus_tag="PLW08_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPL51983.1"
                     /translation="MAHIAGIELPKNKRLFIGLTYIYGIGRSSAREICKKANIDEMKK
                     VADLTMEEEKILRDIIHNEYVVEGALRTQVAMNIKRLMEIGCYRGLRHKRGLPVRGQR
                     THTNARTRKGPRGSTIKKKK"
     gene            12449..12838
                     /gene="rpsK"
                     /locus_tag="PLW08_04940"
     CDS             12449..12838
                     /gene="rpsK"
                     /locus_tag="PLW08_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPL51984.1"
                     /translation="MAAKTRTKKKRVRLAFDEGLFYVHSSFNNTIVTLTDRAGNVLAW
                     SSGGKVGNKGSRKSTPFAAQIAATEVAKAGLDMGIQKVGVIVKGPGGGRESAIRAVNA
                     AGMKVTMIKDATPIPHNGCRPPKTRRI"
     gene            12851..13468
                     /gene="rpsD"
                     /locus_tag="PLW08_04945"
     CDS             12851..13468
                     /gene="rpsD"
                     /locus_tag="PLW08_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPL51985.1"
                     /translation="MAKYTGPKAKICRKFGENIYGNAKYDKILSKRKYPAGQHGRNLR
                     RKLSDYGLHLKEKQKMRHTYCLMEKQFSNYFKKAAKAPGVTGDNLLQLLERRLDNVVY
                     RMGFAVTRMQARQFINHGHFLVNGRKVDIPSYLVKAGDIIEVRPKSKGMKPLLEAMDK
                     TEVTSPYSWLSVDKENFRGQFNSIPAVSEIPVTCDLRLIVEFYSK"
     gene            13494..14669
                     /locus_tag="PLW08_04950"
     CDS             13494..14669
                     /locus_tag="PLW08_04950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPL51986.1"
                     /translation="MMYLEPLQMPNEVDYDKASYSRTYGKFEIGPLEPGYGTTIGNTL
                     RRILLSSIQGAAVRFVRIEGLHHEFCPIPGTNSDYIDLILRLKQLVISSSSVEEVPLV
                     LEHKGKGVITASMIQETAYVKIINKDLYLLEVVDDIDFRLEMIVGIGRGYVSADRQNP
                     EGKAVGFIPVDSIYSPVLKVNFSVSHQRVKERMNFDRLIMEIYTNGAVEPSIALFLAA
                     KILKDMAAKISLFEIEPQYIRDIELDPELEEKERILKTSVKEIELSVRAANCLAQAKI
                     ETIGDLVSKTEAEMLKFRNFGKKSLEEIVQKLKKYDLHLGMDVEGIYRQIREARSRGV
                     IPAEEKEIPAPETKTKTKPKTKTVAEKPAPKEKKTAESVTTATVRKPVPSSKKKVEK"
     gene            14666..15073
                     /gene="rplQ"
                     /locus_tag="PLW08_04955"
     CDS             14666..15073
                     /gene="rplQ"
                     /locus_tag="PLW08_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPL51987.1"
                     /translation="MRHRVEGRKFGREKDARNLMMKNLVKSMIEHGQLKTTLPKAKEL
                     RGYVERVVTYGKKDTVHSRRLAYKVLGDRTLVKKLFNEIAPAFEGRNGGYTRVLKAGF
                     RKGDNAPMAIIQFVEESTVKPKKDAIKAKDLNK"
     assembly_gap    15489..15588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16019..18502)
                     /gene="leuS"
                     /locus_tag="PLW08_04960"
     CDS             complement(16019..18502)
                     /gene="leuS"
                     /locus_tag="PLW08_04960"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPL51988.1"
                     /translation="MEYRFSEIEKKWQNIWRERGIFNAPDRSSKPKYYVLSMFPYPSG
                     VLHIGHISNYSIGDVISRLKMMEGYNVMQPIGYDAFGMPAENYAIEHNSHPRLTTEEN
                     IAAMRIQFDSMGFGFDWSREISTCRPEYYRWGQYLFKKLYEKGLVYRKKGFQNWCDKC
                     QTVLANEQVVDGHCWRCGNIVEQKELEQWYFKITEYAEELLDFSQVIDWPERVITMQK
                     NWIGRSEGAKIDFILEVTNAKIPIFTTRPDTIYGVTFMALPPEHPLVQQWLREEPDNK
                     ELQDFCHKVINEDKVVRSSEETIKEGIFSGRYAINPFNGERIQIWITNYVLMDYGTGA
                     VMAVPAHDQRDFEFAKKYNIPIKIVIQKPDNSLKIEEMQEAYIEPGIMTNSDKFNGMD
                     SEQAKMAITDWAMENGWGEKTVSYRLRDWCISRQRYWGNPIPVIHCPHCGIVLVPDED
                     LPVKLPDNVQVGRTTKNPLLSVPEWINVPCPKCGTPAKRETDTMDTFVDSSWYYARYT
                     DPNNDREPFSKELADYWLPVDQYIGGIEHACMHLLYARFFHKFMRDLGWVNCDEPFAR
                     LLTQGMVTKDGAKMSKSKGNVVDPKYIIDRFGADTLRTFLLFASPPEKDVEWSDDGIM
                     GAFRFLNRVWRLVESNRETIKRGLQTGESSEPLSEEIYNLRYETHYAIFRWREDCLER
                     LQFNTAIASCMEFLNAIAKIKEPDKLNSAELKIYAFACVTLPKMLYPFAPHIAEELWQ
                     ILGNEKMLNECGLPEYEEKYLTRNIVTYVVQVNGKIRGKLEVPIDINPEDLQTKALEI
                     ENVQRFLQGLNIKKIIVVPGKMVSIAAGK"
BASE COUNT         5945 a         3288 c         3927 g         5325 t
ORIGIN      
        1 gcagatttcg cagataaaaa cgcagattta tttttaagtt cttagttctg agttcgtagt
       61 tcttagttct cgggaagtaa gtattatttt tccctttatc ttatttctct ctttttctct
      121 ttttctcttt gtgaaaaatt actcttctca ctttctctct ttttctcttt ctctctttgt
      181 aaaaaattac tcttcacact ttttctcttt ttttcttttt ctctttctct ctttgtaaaa
      241 aattactctt ctcactttct ctctttttct ctttctctct ttctctcttt gtaaaaaata
      301 aatccatccc gtcatctcgc aaaaaaaaga tttgacagtt tctccgtcct aaaatttggt
      361 ggcttaaatt attcattacg ctcatagcgt aaagcacaag tcccatattt tgcaggggct
      421 tatagttctt taggaggaac aatgtcatcc attaaatgtg gtactcacat tgagtatcgc
      481 tgtggaaggg gagttaaccc aatccaccac ttaactaaaa caaaccgcag tttgatttcc
      541 tgcacagatg gcggagctct acaaaagttt cgttttaacc caggggaaac cattttgcat
      601 aaattttgga ggttccagtg agtaaacacg ataaccgaat ccgaataaaa ttgaaagctt
      661 atgatcatcg tcttcttgat aagtcggtgg ctgaaatcgt gaaatccact cgcaatacgg
      721 gagccaaagt tgtcggtcct attccgttac ctacagatag gactatttat acgattttgc
      781 gtagtcccca tgccgacaaa aagagtcaag atcagtttca gatgctggtt cacaagcgtt
      841 taattgatat tgtgaatcct actcagcaaa ctactaatgc acttaagaaa cttagcttgc
      901 ctgcaggtgt ccatgtggaa atcaaagcga acacaaggag ctaaggatga ttggattaat
      961 tggtaaaaaa ataggtatga cacaagtgtt tgacagcgat ggtaaagtaa ttcctgttac
     1021 cgttattaaa gccggaccct gtaaagttat ctgcaaaaga actgaagctg aaaatggcta
     1081 tgaggcaatc caggttggtt atgaagaaat tcctgcccgg aaagtaagta aaccattgca
     1141 gggtcacttt aaaaaataca acagtccttt ttaccgttat gtgcgtgaat ttcgtccttc
     1201 ttacggacaa catattgaaa gtgttcaaat tggggatgaa ctgaaagcag atatttttgg
     1261 agaaaatgaa acggtttctg tttctgccaa ttccaaagga cgcggtttta cgggagtgat
     1321 gaaaaggcat aattttaatg gttttattag ttctcatggc agtcatgagt cctttcgggg
     1381 acctggttcc ataggtcaat gtgctcagcc ctcgcgtgtt tttaaaggaa tgaaaatggc
     1441 tggacaatat ggtaacacga aagttactgt gcatcatctc aaggttgtaa aagttgatcc
     1501 tgaaaataat ataataatgg ttaaaggtgc tgttcccggt catcgtaatt ccttagtaac
     1561 aattcacaag gaacaatagg tggtaactta tgttaacagc aaaacgatat aatatgcaag
     1621 gtgaactttt aggagaagtt gatcttcctg aaggtatctt tgaagtggat gttaatgctc
     1681 ctaagatttt actcaatgaa gtggttacaa tgttccttgc caatcagcgt caaggcacag
     1741 tgcaaaagaa aaatagaagt atgactgctg gaagcacgcg taaacttttc cggcaaaaag
     1801 gaacaggtaa tgccagagtg ggaactcgta gaagtcctat tcgggtgcat gggggaaaag
     1861 cgtttgctat ttatcccaaa gattggtatc gtaaaattcc ccgcaccaaa aaacgcctcg
     1921 ctttgaaagt tgctttaaca gaccgtgctc gcgaaggcag aataatgatt gtagataatc
     1981 taagttttga aaaaccgaat actaaacttg ccgtggaact tttaaacaaa attataccgg
     2041 aaaaaggtta taaactggtt gtaactgatg ggcaccatat tccaactgtt aaaagttttg
     2101 ctaatttgcc tgaggttatg acggacagag cagatagttt gaatgcttat gaagtgctta
     2161 aaagcagtta tataattatg actgatgaag ctctgaaaaa agtggaggag gtattcaaca
     2221 aatgatacac ccacgcaata tcataattag cccgataatc actgaaaaaa gcagtaacca
     2281 ggttcaaaat gaaaatactt acagctttaa agtgagcagt aatgctaata aaatagaaat
     2341 caaaaaagct attgaacgca tttttgccgt taaagttctg gcagtgaata ctattcgcta
     2401 caaagggaaa cctaaaagct taggtaaata taacggcaaa aggcctgact ggaaaaaggc
     2461 aatagtaacc ctgcgcgaag gtgataagat tgccgatttt gaggtttaat gaggtgaata
     2521 atgggaatta aaaaatacag accaacaact tcctccctcc gttttcgcac cggtcatact
     2581 tttgaggaga taactaaaaa gacccccgaa aagtcattac tgaaaccgaa acatagaacc
     2641 ggaggcagaa acaattccgg aagaataacc tgtcgtcata gaggtggagg tcacagacgc
     2701 cactatcgga taattgattt taaacgcgat aaaattggaa ttccggcaaa agttgctgcc
     2761 atagaatatg atcccaatcg gacagcccgt attgctttac tgcattatgt ggatggcgaa
     2821 aaaagatata ttgttgctcc cgatggtttg gaagtaggag ctaaagtgat gagcggtccc
     2881 gatgctgata ttgcagtggg aaacgctatt cctttggaaa agataccctt gggaagcgtt
     2941 gttcataatg tagaattgaa aagaggaaaa ggtggtcaga tagctcgtag cgctggaact
     3001 tttgctcagg ttgtagctaa agatggcgat tatgttcata ttaaaatgcc ttccaatgat
     3061 gtgcatttaa tccggaagga atgtcttgct accataggaa gcgttagcaa tccggatcat
     3121 tctttaattc aaattggcaa agccggacgt aaacgctgga tgggaattcg tccttcagtt
     3181 cgtggtgtag caatgaatcc tgtagatcat cctatgggcg gaggtgaagg aaaaacctcc
     3241 ggaggtggtc atcctgtttc cccttggggt aaacctgcaa agggtggaaa aacacggaaa
     3301 acccataaat acagcgataa atatatcgtg aaagcagtta agaaatagaa taaggataga
     3361 gaaaatggca cgttcaatta aaaaaggtcc ctttgttgat gctcatctgc tgaagaaagt
     3421 agaggtgcta aatgctgaag gtaagaaaag cgtaattaag acctggtctc gccgttctac
     3481 aataactcct caatttattg gtcatacttt cagtattcat aacggacata aatttgttcc
     3541 tgtttatatt actgaaaata tggtaggtca caaattgggt gaattttctc caacccgtac
     3601 ctacagaggc cataaagaaa agaagaaaaa agttacgaga tagggagaga gaaatggaag
     3661 cgaaagcaaa actttgctat gctcgcggct cagcaagaaa ggcacgatta gtgttagata
     3721 cgattcgcta taaacctgta acagaagcac aaaatatatt gcgtttttca cgccgtagag
     3781 tagcaggaac gataaataaa ttattggctt ctgccatagc caatgctcaa catcaggatc
     3841 ctaaagttaa tctggataaa atgtatgtaa taactgccac agctgatgag ggtccgcaaa
     3901 tgaaacgtta tatgccccga gccatgggaa gagcttatat gattaggaaa ccgacctgtc
     3961 atatctccct ggaaatacaa acaatagaag aagaatagga ggaaaacctt gggacaaaaa
     4021 atacatcccg ttctttaccg tctcggtgtt aataaagaaa cagaatccat ctggtttgct
     4081 caaggaacct cgtatgtgga ttttcttcag gaagatatta aaatccggaa ttatattcgc
     4141 aaacgcttga gtgacaagat ggtttcccgt gtgcagattt cgcgtaaaac aagttcaata
     4201 ataattgata tttacactgc ccgtccaggt ttagtgatag gcaagaaagg tgaagatatt
     4261 gataagctgc gtaatgaact gaatgttttt attaacaaag accgaaaaaa tccgatcagc
     4321 gtgtctatca acattgaacc gatagataaa atgtggttgg atgctcgttt ggtggcacaa
     4381 gaaattgcgc gccaactgga agagcgaatt tctttccgca gggctatgaa aatggctatt
     4441 cgttatgtta tgaaagaaaa cgctcagggt gttaaagttc aagtttccgg tcgcttgggt
     4501 ggagctgaaa tagcgcgctc agaaagatat aaacaaggca gaaccccgct tcatactctt
     4561 cgtgccgata ttgattatgc caatgtggaa gcaaatacta cttatggtgt tatcggtgtt
     4621 aaggtttgga tttataaggg tgatatactt gattaaagga gatatgtaat gttagcacct
     4681 aaaaaagtaa aacatcgcaa aatgatgaaa ggaagaagga aaggtctatc ctggaccgga
     4741 tgcaacatct cctttggtga ttatggatta atagcccttg aggactcatt tattactagc
     4801 cgccaaattg aagcatgccg tattgccatt actcgtcata tgaaaagaga aggcaaggtc
     4861 tggattagaa tatttcccga taagcccatt accaaaaaac ctgccgaaac gagaatggga
     4921 aaaggtaaag gcgctccaga atattgggta gcagtagttc gtccgggaag ggttcttttt
     4981 gaaattgaag gtgtggatgt gaatgttgct aaagaagcat tgcgtttagc agcgcacaaa
     5041 cttccgatta agacacgctt tattgctcgc gaaggagtag aattatgaaa atagaggaaa
     5101 ttcgcgattt aagcattgaa gaattgcagg caaaactgga agaattacgc atagagcttt
     5161 ttaatctgcg ttttcaaaag acaaaaaacc tgctggaccg tccagaccgt attcgtaata
     5221 tccgacatga tatagctcgg attaatacta ttttaacaga aagagaaaaa gagaaaaaaa
     5281 tttgaggtga taagtggcaa caacacataa aatgattaaa caaggaattg tcgtaagtga
     5341 taaaaatgat aaaactatcg tggtccgcgt agaacgtcag tttatccatc cgttatataa
     5401 aaaaactgtg cgccgacata aaaaatttat ggctcacgat gaaaataact ctgcccacga
     5461 aggtgatatt gttcaaatta tggaatcacg tccattgagt gcacgcaaac gctggactct
     5521 gcataaaatt gtggaaagaa gcaaataaag gagtttgggg aagatgattc aggttcaaac
     5581 aatgttaaat attgctgata actccggtgc caagaaagct atgtgcataa aagttcttgg
     5641 cggaacacaa cgcaaatatg cttcaattgg agatgttata gttgtagcaa ttaagagtgc
     5701 tactccgggt ggaaaagtaa aaaaaggtag cgtagaaaga gctgtaatag ttcgcacgcg
     5761 caaagaattg cgtcgtccag atggttctta tattcgtttt gccgataatg cagctgttat
     5821 tattgatgaa aaacatgaac cgaaaggaac gcgtattttt ggtcctgtag cccgtgaatt
     5881 gcgtgaagca cattatatga aaatagtatc tctagctccg gaagttctgt aggaggaaag
     5941 gtggcaaaga ataaaatgcg ttttaaaaaa ggtgatctcg ttaaagttat tgcgggtgat
     6001 gaaaaaggta ggaaaggtca tattttgaag gtctttccca aaaccggaaa ggttatcgtg
     6061 gaaaaagtgc atatgattaa aaagcatgcc aaacccacac agcaaaatcc tcaaggtggt
     6121 attataacta tggaagcacc tattgacgct tccaatgtaa tgcttttcaa cgaaaaactg
     6181 aatgctgttt ccaaaccggt ttatcaagtt cgcgaaggac atcgtatccg agtttgcaaa
     6241 aagagcggtg acgagcttta gaggagaaaa ataaatgaac cgtttgaaag aaagatataa
     6301 aacccaggtt atggataact taatgaaaag gttcggttac aaaaatccgc atcaggttcc
     6361 ccgattagaa aagattgtag ttagtatggg agtgggacag gcaactcaaa ataaagctat
     6421 tttagataat gccgttaagg atatggaaac aatttgcggt agaaaaccgg tagttacaaa
     6481 agctcgaaaa tctatctcca acttcaaact acgcaaagga atgcctattg gttgcaaagt
     6541 tactttgaga aatgaagtga tgtatgaatt ttatgatcgc ctaatctcta tagcaatacc
     6601 acgaattcgt gattttcgcg gaattccggc tgattccttt gatggcagag gaaatttttc
     6661 ttttggttta aaagaacaga cagtatttcc ggaaattgaa tatgacaaga tagatatgat
     6721 tcggggatta aatataacca tagttaccac agctaaaacc gatgaagaat gccgtgctct
     6781 tttgcaagag ctgggtatgc ccttccagaa aaacgaataa ggagatatag atggcaaaaa
     6841 agtcactcat catcaaacag caaagaaccc ctaaatataa agtaagaaaa tacaatcgct
     6901 gtaaaatttg cggacgccca cgttcctata tgcgcgattt tggaatgtgc cgtttgtgtt
     6961 tccgtaaata tgcatctgag ggccagatac ctggaatcac cagaagtagc tggtaaaata
     7021 ataggaggaa aataatgagt attagtgatc cgatagctga tgcattgact aaaattcgca
     7081 atgcatatcg tgccggacat acacaggtga ttgttaacca taataaactg attgaagcat
     7141 tggttaacat ccttgcggaa gagaattttg taaatagttt ccaggttttg gataaagacc
     7201 cggagcataa aataaactat aaacgtattt taattattct tcgttatacg aatgccggag
     7261 aacctgtaat acaaggaatt cagagaattt ccaaacccgg aagaagggtt tatgtgaaag
     7321 ccgataaact gcctagcgtt tataataata ctggttgtgc agttatttct accagtatgg
     7381 gtttaatggt agatcgtgat gctcgcctga aaaatgtagg cggcgaatat atttgtaagg
     7441 tttggtaagg gggaaaaatg tcacgaattg gaagagcccc gatcaaatta caacctggaa
     7501 cgcaagtgga tgtagtggat aacaaaatta ccgttaaggg aaaattggga gaattgaatt
     7561 ggtctttgct tcccggaatt actgttgaag tagaagaaaa ccttctgaaa gtgaaaagag
     7621 cagacgatag taaaaaccag cgtgcttttc atggtcttag tcgtgcttta atacaaaata
     7681 tgattaccgg tgttacagaa ggatttcaaa agatattgca tatctatgga acaggttatt
     7741 ctgcggaagt tgttggacca tggctaaagc taactcttgg tttttcccat gatattttgt
     7801 tacaaattcc tgaaggattg gaagttacta cagaagctgt tccgcgttct aaaggaatgc
     7861 gttccgattt tcaaagtata attactatca aaggtattga taaacagttg gtaggcgaat
     7921 ttgctgccga agttcgcggt tgccgtcctc ccgaaaatta taaagggaaa ggcataaggt
     7981 attttgatga gtatgttata attaaagctg gaaaagccgg aaccaaataa gggagagggg
     8041 aaaagatgat aacttcaagc tgtaaaatta aaagagacct ccgaaatcgt cgtcatttat
     8101 ctattcggaa acgcgtaagc ggaactcctg aacgtcctcg tttagtagtt tttcgttccc
     8161 tgaagaatat atatggtcaa attatagacg atacacaggg tgtaacttta gtttcaatgt
     8221 ctactattgc caaagatata ggttccttaa ccggaaagaa gaaaacggaa cagagctatg
     8281 aagtaggtgt aaaacttggt gaaaaagcac ttgctgcagg aattacaaaa gttacttttg
     8341 atcgtgccgg atacaaatat cacggtagag tgaaagctct tgctgaaggt gccagaaaag
     8401 ctggtttgga attttaagga gcaaaaatga attacgaaca aaatcatcca gatgaagaaa
     8461 aattgataga aaagatcgtt gaaacaaagc gtgttgccaa agttgttaaa ggaggcagga
     8521 acttcagttt taccgcaata gttgtagttg gtgacaaaca aggaaatgtt ggtgtgggaa
     8581 atggcaaagc aaacgaaatt gtggatgcca ttcgaaaagc caaagaaaaa gccgtaaaaa
     8641 atatgtttaa agttccgatt gtgaaaggaa ctgttcctca tgagattgta gctcgttatg
     8701 gcgcttctaa agttttaata aaacctgcag ctcctggcac gggaattatt gcaggaggaa
     8761 ctgccagagc catttttgag gcagcaggaa ttgaaaacat tttgtgcaaa tccttgggct
     8821 ctaacactcc tactaatgta gtaaaagcta ctatcaacgg tttgaaatca atgcgtactt
     8881 tatcagatat ttcacgcttg agaaataaaa caatggctca acttactggt caggaggaga
     8941 aatgaaattg aaagttactc aaacccgcag tactataaat cgtattgaag accataaaaa
     9001 ggttattaaa gccctcggtt tggggaaaat aggtaagagc cgtattcata atgatactcc
     9061 atcccttaga ggtatgatca ataaggttgc ttacctctta aaagttgaag aactgaagga
     9121 agaatagcga tgttaacttt atccgattta gaaagaccta gttgcaagaa aaacaaaaaa
     9181 cgcttgggaa aaggacaagg ttccggatgg ggacatcaag ccggacgtgg tcataaaggt
     9241 aaaaaagcac gtgccggtgg aaatgtgcct cctcgttttg agggtggtca aatgccttta
     9301 aacagaagag ttccaaagcg tggtttcaaa aatatcttcc gtgaaaatta tcgcgtactg
     9361 aatctttatc gtttacagga cttggatatt actgaacttg atattcctac gatggaaaag
     9421 ctgggtttaa ttcccggtaa aggcaaaaag gcaaatgcac cggttaaagt tctggctggt
     9481 gtaaaagatg aattcactaa aaccgttact atcaaagcca atgctttttc acaaaaagcg
     9541 aaagagatta ttgaaaagaa cggtggtaaa gcggaggtag tgtaacttgt tcaagacgat
     9601 tggtaatatg ttccggattc cggaccttaa gaagaagatc ttatttacag ctctctttct
     9661 ggttttatac cgtttgggaa gtttcattcc gcttcccgga gtgaatgcca ctgctcttaa
     9721 agcttttttt gaaggtgctc gtgaaggtgg aaatactctt tttggtctat tagatctttt
     9781 tgtgggtggc agttttgaaa gagcatctgt ctttgcctta ggtattatgc cttatattac
     9841 ggcttccatt gttattcagc tgttgggaag cattattccc tattttgaaa aattgcgtaa
     9901 agaaggagct gatggccaga agaaattgaa tcagattacc cgttatggaa cagttgcatt
     9961 ggctgctttt aatgccataa ctattacaat gtggcttacc aatctttccg gtggagttgt
    10021 gccgcgtcca aatttcttgt ttcatttaac cgccataata actttggtaa cgggaacgat
    10081 gattgttatg tggattgggg aacaaattac cgagcatgga attggtaatg gtatttcact
    10141 aatcattttt gccggtatta ttgctcgtta tcctgaaggt tttgtaagga tgattcaaaa
    10201 tattcccggt aatccttctt atgtatggaa ggcaattatt tctgttgttt taatggtttt
    10261 agtaactgca gccgttatct tcgttacgga ggcagttcgt aaaataccgg ttcaatatgc
    10321 caagcggata gtaggaagaa gagtttatgg aggtcaaagt acctatattc ctttgcgggt
    10381 gaatactgct ggagttattc ctattatctt tgctcagagt gttttaatgt ttccggcaac
    10441 gatagcggct cttttaggag gggatagtta tttttggact accttgagaa attggttgtc
    10501 gccaggagct gttttataca ctattttata tgttgggctt attattttct ttgcctattt
    10561 ttacactgcc atagtgctca atcctacaga aatggcagaa aatatggtta aatacggtgg
    10621 tcacattccc gggaaaaaac ccggtaaaaa gaccgctgaa tacattaaca gtgttcttgt
    10681 tcggataacg cttccgggag ctattttctt tgcctttatt gccttgttgc cggaaataat
    10741 gagcaaccgt ttagacctgc ctttttattt tggaggaaca ggtcttttga ttgtggttgg
    10801 tgtagctttg gatactttgc aacagattga atcccatctt gttatgcgtc actatgatgg
    10861 ttttatgaag aaaggtaaat taaggggacg ctccagttaa gatgatatac atcaaaacgc
    10921 ctgcagagat tgaaaaaatg cgcaaaagtt gtcgcatcat tggtgaatta ttagatgctc
    10981 tggaaaccat aatccgtcct ggaatcagta ctttggaaat agataaattt gctgaggatt
    11041 ttattggcag tagaggtggc aaaccttcct tcaaaggata tcgggtagat ggtttacctc
    11101 cttttccagg ttctatttgt gcttctgtaa acgagcagat tgttcatggc attcccagcg
    11161 ctaaaaagat tttgcaggat ggtgatatta tcggtattga tgtgggagtt gttttggatg
    11221 gttataatgg agatgcggca agaacttata aagtcggcaa aatttctgct gaagcagaag
    11281 aacttttgca ggtaactaaa gaagctctgg aaagaggaat ttctgctgct attccgggaa
    11341 accgtgtggg cgatatttct tatgagattg gttcttttgt tgcctttagg ggttattatg
    11401 ttgcagataa tcttacgggt catggagtag gacgcaattt gcatgaggaa ccacaaattc
    11461 cgaatacagg aaaaccggga agaggtcctc gtttatctgc aggtatgact ttagctattg
    11521 agccaatggt taatattggc acgaatcgtg tattagagaa tggttgggaa tttaaagtgg
    11581 ctgataatag tttatctgcc cattttgaac ataccatttt agttaccgat aacaaaccag
    11641 aaatattaac agttgctgat tgaggaatat tatactatga gtaaagctgg tgttatagaa
    11701 gtggaaggtg ttgtaaccga agcacttcct aatacaacct ttcgtgttac tctggaaaat
    11761 ggccatgaaa ttttggctca ttcttccggg aaaatgcgaa tgaactatat cagaattctg
    11821 cctggtgata aagtgaaggt tgaactttca ccttatgact taacgcgggg cagaatcacc
    11881 taccgctata aataattata aatgattgca aacagccatc ttggctaaag caaggagatg
    11941 ataatgaaag tgaaagcttc cgttaaaaaa atctgtaagg attgcaaaat tattaaacgc
    12001 aacggtatta tccgtgttat ttgcagttca aatccaaaac ataaacagag acagggttaa
    12061 ggaggaaaaa cttggcacat atagcaggta ttgaacttcc caaaaacaaa cgtctgttta
    12121 tagggttaac ttatatctat ggcattggca ggtcaagtgc cagagaaatt tgtaagaagg
    12181 cgaatattga cgaaatgaaa aaagtagccg atcttacgat ggaagaagaa aaaatcctac
    12241 gcgacataat tcataatgaa tatgtggttg aaggagcact tcgcactcaa gtagctatga
    12301 atattaaacg cttgatggaa attggttgtt accgcggttt acgccataaa cgcggacttc
    12361 ctgtccgagg acaaagaaca catactaatg ccagaaccag aaaaggtcct cggggtagca
    12421 caattaagaa aaagaaatag gagaaattat ggctgctaaa acaagaacca agaaaaaaag
    12481 agtgcgtctt gcttttgatg agggactttt ctatgtccat tccagtttca ataatactat
    12541 tgttacttta accgatcgtg caggaaatgt tcttgcctgg tcaagtggtg gaaaagttgg
    12601 taacaagggc tctcgtaaaa gtacaccttt tgcagctcaa attgccgcta cagaagttgc
    12661 taaagcaggt ctggatatgg gaattcagaa agttggggtg attgttaaag gtcccggtgg
    12721 aggtagagaa tccgctatca gggctgtaaa tgctgcagga atgaaagtta ctatgattaa
    12781 agatgcaact cctattccgc ataatggctg ccgtccaccc aaaaccagaa ggatataagg
    12841 agagtaacaa atggctaaat atacaggacc caaagcaaaa atatgtcgta aattcgggga
    12901 gaatatttat ggcaacgcca aatatgataa aatactcagc aagcgtaaat atcctgccgg
    12961 acagcatggc agaaatttaa ggcgtaagct ttctgattat ggtttgcatc ttaaggagaa
    13021 acaaaaaatg cgccatactt attgcttaat ggaaaagcaa ttcagtaact atttcaaaaa
    13081 agctgccaaa gcacctggag ttacaggcga taacttattg caattattgg aacgccgttt
    13141 ggataatgta gtttatcgta tgggttttgc tgtaacaaga atgcaggcgc gtcagtttat
    13201 caatcacggt cattttttag ttaatggcag gaaagttgac attccttcct atttagttaa
    13261 ggcaggagat attattgaag ttagacccaa aagtaaaggg atgaaacctc ttttggaagc
    13321 aatggataaa acagaagtta cttcgccata ttcctggctg agtgttgata aagaaaattt
    13381 ccgcggtcaa tttaattcaa ttcccgcagt aagcgagatt ccggtcactt gtgacctgcg
    13441 tcttattgtt gaattttact ccaaataagg cgctataagc ataaggagca gctatgatgt
    13501 atcttgaacc tttacaaatg ccgaatgaag ttgattacga taaagcatcg tattcacgca
    13561 cttacggcaa gtttgaaatc ggtcctttgg aaccgggata tggcaccaca ataggaaata
    13621 ccttgcgtcg gattttgctc tcttccatac aaggagccgc tgttcggttc gttagaattg
    13681 aaggtttgca tcatgaattt tgtcctattc cgggaacaaa ttctgattat atagatttaa
    13741 ttctgcggtt aaaacagctc gttatctctt ccagttctgt ggaagaagtt cctctggttc
    13801 ttgaacataa aggaaaaggc gtaataactg cctctatgat tcaggagacc gcttatgtga
    13861 aaattatcaa taaagacctc tatctgctgg aagttgttga tgatattgat tttcgtttgg
    13921 aaatgattgt aggcataggg cgcggttatg tttcagcaga caggcaaaat cctgaaggta
    13981 aggcagttgg ttttattcct gtagattcca tttattcacc tgttctgaaa gtgaacttca
    14041 gcgtttccca tcaacgggtg aaagagagaa tgaattttga ccgtttgatt atggaaattt
    14101 atacgaacgg agccgtagag ccatctattg ctctatttct tgcggctaaa attttgaagg
    14161 atatggcagc taagatatct ctgtttgaaa ttgaaccaca atatattcgg gatatagaat
    14221 tagaccccga gttggaagaa aaagaacgca ttctgaaaac aagtgtgaag gaaattgaac
    14281 ttagcgttcg cgctgccaat tgtctggctc aagcaaaaat tgaaaccatt ggcgatttgg
    14341 tttccaaaac ggaagccgaa atgctgaaat tccgtaattt tggcaaaaaa tcactggaag
    14401 aaatagttca gaaactgaag aaatatgatt tacatctcgg tatggatgtt gaaggtatct
    14461 atcgtcaaat tagagaagcc agaagcagag gagtaattcc tgctgaagaa aaagaaattc
    14521 cggctccgga aacaaagaca aaaaccaaac cgaaaactaa aacagttgct gaaaaaccag
    14581 cacctaaaga aaaaaagaca gctgaatctg taactactgc aacggtgcgt aaacccgttc
    14641 ccagttctaa aaagaaggtg gaaaaatgag acacagagtt gaaggaagaa aatttggtcg
    14701 ggaaaaagat gcccgcaatt tgatgatgaa aaatcttgta aaatcaatga tagaacacgg
    14761 gcaactgaaa acaacccttc ctaaagctaa agaattgaga ggatatgttg aacgtgttgt
    14821 tacttatggc aaaaaagata cagtccattc cagacgtctg gcttataaag tattgggcga
    14881 tagaacactg gttaagaaac tatttaacga aattgctcca gcttttgaag gtcgtaacgg
    14941 tggctataca cgtgtgttaa aagctggttt ccgcaaaggg gataatgcgc ctatggctat
    15001 tattcaattt gtggaagaat ctactgtgaa acctaaaaag gatgccatca aagctaaaga
    15061 cctgaataaa taaaaaaact ctcattgaat acacggattt ggattcacaa agagataaag
    15121 agaattaatg aagaatgggg aatggggaat tgagaatgga aaatggaaat ttgagaatgt
    15181 aagtaagtca ggagtcgtca tcgctgatat aaattattac ctatcctcaa acttatttag
    15241 cgatgaggac tcgtgacgat tttgattatg tagccggagg acgctgttcc tccgcaattt
    15301 ttaaccaccg aaaatcccca aaaatactct catagatttc agggatattt ttagttaata
    15361 ggtcaggaat gaacaaggaa aaagggagtt tagggttatt tttaattata ttcaaaaata
    15421 ttatgctttc tgtttatgcc aaaccggaat tcggcatccc atttataaat gtgccttata
    15481 actatttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt atgccaaacc
    15601 ggaattcggc atcccattta taaatgtgcc ttataactat ttccaaacca tagttggggc
    15661 ttattttctg cataattatt tgtggtagat atagagtggc ttatctcggt aatttgccga
    15721 gaggcgtcaa gatattgcta acgcagataa ttaagtatga tgcttttcct aattattgaa
    15781 attctcgtca acccctgtca accaagcata gaatcatatt atttcttaaa tgaaaagacc
    15841 cttgtcattt ctacgcaagc aggaatctgg ataagttccg atataataat tgcattttcc
    15901 agatgaaaac tatattattc ccgctttttt acatcgggga caccgagaat actgaaaaca
    15961 ccgagatatt tttagggtct ctcattatat tcatttttta tgcttcattc ttaattcact
    16021 attttcctgc ggcaatagaa accatttttc ccggcaccac tattatcttt ttaatgttca
    16081 gtccctgcag aaagcgctgc acattttcaa tttccagggc tttggtttgt aagtcctccg
    16141 gatttatatc aatcggaact tccagtttac ctcttatttt tccgtttacc tgaaccacat
    16201 aagtaacaat attgcgggtt aagtattttt cttcatattc gggaagtccg cattcattca
    16261 gcattttttc atttcccaat atctgccaaa gttcttccgc aatatgagga gcaaagggat
    16321 aaagcatttt aggcaaagtg acgcaggcaa aagcgtaaat ctttaattcc gcagagttca
    16381 atttatctgg ttctttaatt ttcgctatgg cattgagaaa ttccatacag cttgcaatag
    16441 cggtattgaa ttggagacgc tctaaacaat cctctctcca gcgaaatatg gcataatggg
    16501 tttcataacg caagttatat atttcctctg aaagaggttc agaactttca cctgtctgca
    16561 aaccgcgttt tatggtttcc cgattacttt ctaccaaacg ccagacccgg ttcaaaaatc
    16621 tgaaggcacc cataattcca tcatcgctcc attccacatc tttttcgggg ggtgaggcaa
    16681 aaagcaaaaa agtgcgtaaa gtatctgcac caaagcgatc tataatatat ttcggatcaa
    16741 ctacattacc tttggatttg ctcattttag ctccatcctt tgtaaccatc ccctgtgtaa
    16801 gtaaacgtgc aaagggttca tcacaattta cccaacccaa atcccgcata aatttgtgga
    16861 aaaaacgggc ataaagtaaa tgcatacagg catgttcaat tccaccaatg tattgatcta
    16921 cgggaagcca ataatcagcc agttctttgc taaagggctc tctatcgtta ttcggatcgg
    16981 tataacgagc ataataccaa ctgctgtcta caaaagtatc cattgtatcc gtttctcttt
    17041 ttgcgggagt tccgcatttc ggacagggca catttatcca ttccggaacg ctcaaaagcg
    17101 gatttttggt tgttctgcct acttggacat tatccggcaa tttaacaggt aaatcctcat
    17161 cgggaactaa aactatccca caatgcggac aatgaataac cggaatggga tttccccaat
    17221 aacgctgacg cgaaatacac caatccctta aacgataact aacggttttt tcaccccaac
    17281 cattttccat agcccaatcg gttattgcca ttttggcttg ttcactatcc attccgttga
    17341 atttatcgga attagtcatt attccgggtt ctatatatgc ttcttgcatc tcttcaattt
    17401 ttaaggagtt atcaggtttt tgaataacta ttttaatcgg gatattgtat tttttagcaa
    17461 attcaaaatc gcgttgatcg tgtgccggaa cagccataac tgctcccgtt ccgtaatcca
    17521 taagcacata attagtaatc cagatttgaa ttctctcgcc attaaaagga tttatagcat
    17581 aacgaccact aaaaatgcct tctttaattg tttcctcaga actgcgcaca actttatcct
    17641 cattaataac tttgtggcaa aaatcctgca gttctttatt atccggttct tccctcagcc
    17701 actgttgaac taaaggatgc tccggcggca acgccataaa agttaccccg taaatagtat
    17761 ccggacgggt ggtaaaaatc gggatttttg cgtttgttac ttccaaaata aaatcaattt
    17821 tagcaccttc cgaacgtcct atccaattct tttgcatcgt gatcactcgt tcaggccaat
    17881 caatcacttg ggaaaaatcc aatagttctt ccgcatattc tgtaatcttg aaataccatt
    17941 gttccagttc tttctgctct acaatgtttc cacagcgcca acaatgacca tccacaacct
    18001 gttcgttagc caaaacggtt tgacatttat cacaccagtt ctgaaaccct tttttccggt
    18061 aaactaaccc tttttcgtat aatttcttga aaagatactg tccccaacga taatactccg
    18121 gacgacaagt gcttatttct ctgctccaat caaaaccaaa acccatactg tcaaattgaa
    18181 tacgcatcgc tgctatgttt tcttcagtag taaggcgcgg atgggaattg tgctcaatag
    18241 cataattttc cgcaggcata ccaaaagcat cataaccaat gggctgcatc acattatatc
    18301 cttccatcat tttcaatcgc gaaatcacat ctccaataga ataattggaa atatgaccta
    18361 tatgcaaaac tcccgaagga taaggaaaca ttgaaagaac ataatatttg ggcttggaac
    18421 ttctatcagg tgcattaaat atccctctct cacgccaaat attctgccat ttcttttcaa
    18481 tttcactaaa tcggtattcc attttatctc tcccggtgta tcattttatc ttataatacc
    18541 ttgcaaaatt tgggagaccg cataattgtc aagcaaaagc tgtaa
//
LOCUS       DAOOZV010000036        17741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3278_length_17741_cov_53.693317, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000036 DAOOZV010000000
VERSION     DAOOZV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17741
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_17741_cov_53.693317"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..2371
                     /gene="gyrB"
                     /locus_tag="PLW08_04965"
     CDS             473..2371
                     /gene="gyrB"
                     /locus_tag="PLW08_04965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPL51989.1"
                     /translation="MQSSTYTASNIKVLKGLEAVRKRPSMYIGGTSERGLHHLVYEVI
                     DNSIDEALAGYCDEIICTITSEGNIRVEDNGRGIPVDIQKDMGIPAVQVVLTVLHAGG
                     KFDDKSYKVAGGLHGVGVSVVNALSEFLEVNIFKDGKEYFMRFERGLPVTELQVLGDT
                     NKTGTVVTFRPDPLIFETTEFSFDYLTSRLRELAFLNSGLRIILKDERSGRAHDFKYE
                     GGIKSFVEYLNQNKKPLFPNPIYISSVKEGMEFEVALQYNEGYQENIFSFANNINTTE
                     GGTHLSGFKSGLTRAVNTYIKNADLLKNEKVSPSGEDIREGLTAVISLKIANPQFEGQ
                     TKTKLQNSEVEGIVNSAVYEKLMEYFEEHPAEAKNITMKSILAARSREAARKARELTR
                     RKTVLESGSLPGKLADCTLNDPEKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKML
                     NTEKARVDKVLNNEKIQPIILALGAGIGQEFDISKLRYHKIIIMADADVDGSHIATLL
                     MTFFYRYMRPVIEEGKLYIAMPPLFQVRKGKQKVYVYNEEERDKAIQEFGEKGVIVQR
                     YKGLGEMNAEQLWETTLDPKNRKMIAIKMDDAIEADRMFTILMGDEVEPRREFIQENA
                     RYVKNLDV"
     gene            2385..4991
                     /gene="gyrA"
                     /locus_tag="PLW08_04970"
     CDS             2385..4991
                     /gene="gyrA"
                     /locus_tag="PLW08_04970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPL51990.1"
                     /translation="MSETTNIIPVQIEEQLKQAYLDYSMSVIVSRALPDIRDGLKPSQ
                     RRIIYAMHELNLTPGSHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWIMRYPLI
                     EGQGNFGSIDGDPPAAMRYTEARLQKITMEMLTELEKDTVEFRSNYDETRQEPTVFPA
                     RFPNLLVNGSSGIAVGMATNMPPHNIGEVCDAIIALIDNPDLQPLELLEYIKGPDFPT
                     GGFILGTEGIREYFETGRGKIIMRGETEIETLPNDLEQIIIRSIPYQLSTSQLIERMV
                     ELVKEKKIEGISDIRDESGRDGMRIVIQVKRNHDAQVVRNLLYKYTQLQSTFGVINLC
                     LIDGVPQVMNMKEMLQNFIDFRHEVVLRRTQFELRNAKERLHILEGLKIALDNLDEVI
                     ALIRASQTPNDASVGLQEKFGLSEIQAKAILDMRLQRLTGLERDKIETEYQELLIIIA
                     RLTELVEHKELRMNLIKEETAEIRDKFADKRLTTIIPTPAGSFNMEDLIADEMVVVTI
                     THDGYVKRLPVDTYKVQARGGKGLSASNLKEEDFIQYLFVASTHSYILLFTDHGMCYW
                     LKVFEIPEASRTARGKAIVNLVKLGEGDRIKAFVTLKDFHPEQNIVMCTRNGLVKKTP
                     LTAFSHPRSIGIKAIKLREGDELIDARICEAGDDILLATHEGKCNRFSESEIRSMRRF
                     SQGVKGIRLRNGDYVISMTIATAQDQLENGKPNSSTILAISEKGYGKRSPISSYSVTR
                     RGSMGVITLKTNERNGYLAALMLVRDDDDLMIITQGGMIIRQRVSEISVVGRNTLGVR
                     LINLAPNDVVRDISLVHSEPDEESLDKEVETLKAKPASNLEEFADVEDEEFAEEEMDE
                     LEEDLENEEDNE"
     gene            4972..5550
                     /locus_tag="PLW08_04975"
     CDS             4972..5550
                     /locus_tag="PLW08_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51991.1"
                     /translation="MKRITNKHCRMMLTAGILFLLSACSVSQFVPGEGVVSENNYAII
                     RADTLLIIVKPQSYPGSYQEINNRFFSVFISIKNNSRQKIKLQESSFSILCNEKQYDP
                     VPVDYILSDLERRMMLKEFSDPFSPTTEFGITDRNKEQEMYYELVNNAFSSGELLPGA
                     KKEGYLFYNREIASARYFTINILGTAIPFVKQ"
     assembly_gap    5851..5950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6254..7039)
                     /locus_tag="PLW08_04980"
     CDS             complement(6254..7039)
                     /locus_tag="PLW08_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPL51992.1"
                     /translation="MSYSKYFSAEGFCLYLGNCIEILEELEPNSIDMIFADPPYFLSD
                     GTITCKSGKMVSVKKGDWDLLDEFNNKSEFHHKWISVCRQILKPSGTIWISGTYHSIY
                     QCGYELQKQGFRIINDICWFKPNAAPNLTRKCFTASHETILWAIKDPLQKQKYHYELM
                     KNTDWMGDIINKKGKPMRSVWCIPTTPAREKIHGKHPTQKPIALLERIILSSTDEGDL
                     VLDPFNGSGTTGVVAKIYHRNYIGIDINIDYLKLTIERIKDTL"
     gene            complement(7042..7941)
                     /locus_tag="PLW08_04985"
     CDS             complement(7042..7941)
                     /locus_tag="PLW08_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPL51993.1"
                     /translation="MKQLPLYKKIKLNSPEKVFNYLINTMLPTINNWSYLVDWEKVSK
                     HIKDIEICLNTMNYLIGKKNIYNEFQYLLKKHPEIKHILPVLVACRKDSITLLTSNDE
                     NDLQAKEFNFKNLSDEDALNFADRAGLLQLLSSNKIKNLVDYVFGIEVGLDSNARKNR
                     AGKAMEQLMNKHIKKICEKNNIEYLASANKSKVEKQWGIDLPVDKTNRKVDFIIKKDR
                     QLYLIEVNFYSVGGSKLKSTAGEYKNIYNFWKSHNLQFIWITDGWGWKTTQRPLKEAF
                     NELDYILNIKMVLDGVLEYILTN"
     gene            complement(8030..9007)
                     /locus_tag="PLW08_04990"
     CDS             complement(8030..9007)
                     /locus_tag="PLW08_04990"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPL51994.1"
                     /translation="MKDQILSAVNLKEYMPTTQMKLFNFDAKPFLKWAGGKGQLIPEL
                     VDRLPNKFKKSMHIPVYIEPFIGGGAFFFYLQSCFQIDKSILIDINRDLIITYQVIKY
                     AVENLIALLSELDNKYNNASQTERTTLYYDIRKRFNYSKKHLDTTYNELWIRHTAYMI
                     FLNRTCFNGLYRENKNGEFNVPHGRYDKPSICQAENLWDANKALSNTEILQGDFSLAA
                     NYVEENTLIYYDPPYRPLTKTSNFNSYAKEGFADKDQIRLANFFKDMDRPDVYQILSN
                     SDPRVTNPEDDFFDNLYKEYKISRIQAKRMINSDAEKRGNITEILVTNY"
     gene            complement(9148..10080)
                     /locus_tag="PLW08_04995"
     CDS             complement(9148..10080)
                     /locus_tag="PLW08_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL51995.1"
                     /translation="MKRFIFFFLCLLMCISLFAIDNKFLHQLVQVKSSQAYSIRFDDD
                     LVYTNNQNYIWVYSIFNAWQPKLEAAFFSPNPIEDIETLAGKHLYVASNEPTNQVILI
                     DSLYTGSRIFFPQTIVGDKLTREGSILYVADRYRGIDIINLSGGSTRELLATFSEKWG
                     IKDFVASYPYIFALNDFGLVAVDITDQSYPVSLGVNYEIIDATCLVKNGNTIWIGAGK
                     NLLAFNVYEPKKPTLISQIRMTNEILSLDIKDNRLFIALGRGGVKIIDVTNPLKTEDI
                     NNIFLTIPVYDLDVANDYIFLGLGKEGWMLYEYR"
     gene            complement(10077..11063)
                     /locus_tag="PLW08_05000"
     CDS             complement(10077..11063)
                     /locus_tag="PLW08_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPL51996.1"
                     /translation="MTKEELIQDITAMQSLKETLLNEIHNVIIGQDRVLTDVLIALFA
                     NGHILLEGVPGLAKTLLISSLAKALSLSFSRIQFTPDLMPSDITGTEILVNDPVTEKK
                     HFEYIKGPIFANIILADEINRTPPKTQAALLQAMQEYAVTSGTVTRPLSKPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMFFINIDYPTLEEELQIAESTTGLENPVITPQLTGEQII
                     SLQQAVRSLPVSDHVLKFAVNLVRKSRPNTDDALNEIKQWVAWGAGPRASQYLILSAK
                     ARAALDGRLTPAEEDVKASAINVLQHRILPSFAAQAEGINSKQIINWLLEQK"
     gene            complement(11056..12060)
                     /locus_tag="PLW08_05005"
     CDS             complement(11056..12060)
                     /locus_tag="PLW08_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPL51997.1"
                     /translation="MTRKNRNSLVIGLIIGILLLAAWLYYTPLEEIKSQIAQVNYNWV
                     IIASVAYLSAYFLRSLRWRLLIPAPANPGIFKTWLYAMAGNLLNYLIPIRAGDFARAW
                     FIKKNYSLPYMKVLPSVFVDKVFDTIAILFIIIVLPFTTVELSTPMVILLCLLLLIFI
                     VAFSILLISVWKKELAVKIISSLFSWLPQKAKAKINPAIEMFISELNLFEHHPFKLVS
                     ALFLTAGGVLLDGIYFYLLFRAFGILYPFALVLFGYTLINLSYAIPQPPAQLGSNEWM
                     MIIIFSIGFGLTKTTASAIMAFGHILTAGLMSLWGIIAFAVLGPELLFTAIKGDKIND
                     "
     gene            complement(12057..12896)
                     /locus_tag="PLW08_05010"
     CDS             complement(12057..12896)
                     /locus_tag="PLW08_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPL51998.1"
                     /translation="MAVPIINDWAKYFDNPDEGLGSSYERIVLNKLLDFVCKTYKIQS
                     ALETPCFGFTGVSGINLLNLARQGIKVSLEDHNLERIEKIRELWQELRLHTDIKYNLD
                     YVKLDYPDKHFDLGFNFSALWFTQNIRSFLSEFCRVCKKAILICVPNRNGIGYKMQIK
                     DYSPEKYPFLHPEYLDPATIKFLMKQNKWQLQDENYIDCPPWPDIGMKKELFFNRLLK
                     KQEIQENNTEPVTILTYYQDEDSEFAEKMFKYSFVERTAPKRFKKYWAHHYYLLFTPE
                     NSE"
     gene            complement(12896..>13619)
                     /locus_tag="PLW08_05015"
     CDS             complement(12896..>13619)
                     /locus_tag="PLW08_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL51999.1"
                     /translation="PQETYLRSNVYSTGQMVEYCLAKDARLIYCSSVGVFGTIPQELP
                     AGPQTEKNPDNYYHYTKIESERIINRAILNGLNAGIVRPSIAYGEGDFGFPYQMVKLV
                     DKHLFPLINRPIWIHLCHIDALVKAFLYLATHEWKSGLTLTVADREPVRLQSLVDFIS
                     RQLHGKNYPRALRFDRIFFAWGEKLARLVKNELWISRFELISKSWFYDVSAYYDIMEK
                     EGYKSHFTIPEFEITIKDYQKR"
     assembly_gap    13620..13719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13720..14034)
                     /locus_tag="PLW08_05020"
     CDS             complement(<13720..14034)
                     /locus_tag="PLW08_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPL52000.1"
                     /translation="MAKILITGITGFIGRNVLREMLKSCPDFNITALVRPKTDPRRYQ
                     EFEKAIRLVAIDLADTASLKEFLFTNEFDTILHIGALRGGRKYPQETYLRSNVYSTGQ
                     MVE"
     gene            complement(14027..14818)
                     /locus_tag="PLW08_05025"
     CDS             complement(14027..14818)
                     /locus_tag="PLW08_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HPL52001.1"
                     /translation="MVDIHTHLLPNIDDGSKSLNDSLSQLCQMAEGGIKRVYLTAHYF
                     KGHYQYSRQEYDEKFNTIVNKVKDAGIDLDIQPGFEIYLQPDILEDIKTHNLTMGKSS
                     YILLESDLNGLPSDFYNNIFPLLRAGYKPILAHAERYVSIMQKPSRAEDLIYRNIYMQ
                     TNAGSLLGLYGEKVKSTAWILVNKGWTHFIASDDHIRGKYLAYFEARDKVEEMIDNHT
                     AHLLFYVFPSCIASGENIPYEYVQVYRPHHSHHHHRRSLIKRIFG"
     gene            complement(14822..15703)
                     /locus_tag="PLW08_05030"
     CDS             complement(14822..15703)
                     /locus_tag="PLW08_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPL52002.1"
                     /translation="MLLQKYFETRFELIDQELKNALTFEDKYATELKKAIYEAVIPGG
                     KRWRPLLLLSMFEMLTGFKKHRAFPDALKSSVAIELLHNGAIVHDDLPSVMNRTKRRG
                     QPALHIKYGNAIAILAADALYSLAYEIISELKNPEQSLEAIHILSNATKSYGMIGGQV
                     VALSSRHKVMKINTLNYIDMKKVGSLLQAAADIACLLSSADEETHQVMRAYALNLGIA
                     YQMIEDIEADYSRGSEGLDEEYVPVSKASYTALLGFDKARKTVENMLADSAKMLKPYP
                     NNSVLLEFVTMIKERLP"
     gene            16665..17570
                     /locus_tag="PLW08_05035"
     CDS             16665..17570
                     /locus_tag="PLW08_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52003.1"
                     /translation="MRKYRLILCLLLIIIELSAFQVYLPEGQVKEITKEDLSKEKLVS
                     FTTIRDKDGGQKEETWQGIPLIPWLNSLTSSPWQNLQIESNDNFMLTFHRLELDDNNA
                     YLALKLGAEELSEYNIRLIFTRYRENAWMRNIRCLRLLNFIPVPKPKQIYFWEKGLQK
                     NGIDLKSGAIAMKDLMQKGFSLESGDLIFVDEQFRKLCLNYPREIADASLFMDSRGQY
                     FLVPESSSALKISFENDLSRIIYIQCGAIAYLAEDAIENMEKIARTLEWDWQGDYLYL
                     IKAEKQKIKAGEKPMDINADCWLEF"
BASE COUNT         5639 a         3145 c         3441 g         5316 t
ORIGIN      
        1 gaagttgccg acccattttt attcaaccgc attattgggg ataaaaatta aaggttatag
       61 cagaatgcct ataattgtca ctctttattg aagttatata ttcttcctta ggagcattgc
      121 ttacccattg cttacccatt gctaatccca tccgtaatgg gtgagcaatg agtaggtagt
      181 gctattatag aagaaaagca actttcacaa tgacagcaaa ttaacctgaa agggagaaaa
      241 aatgggcgga aaaaaaggga attttaatgc catctaaaaa acaaaaaaaa cgggttttat
      301 aacatattgc aagataaagg attataaata gaaaatagat aaagaaagtg cttgacaata
      361 acactatatc atttactgtg gcaacaaatt aaggattcaa attccagtga gattcattta
      421 tctggaaaaa aatccttagc tttattcata aaattatagg agacaataga atatgcaaag
      481 ctcaacttat acagccagca atattaaagt gctgaagggt ctggaagctg tccgaaaaag
      541 gccttcaatg tatattggag gcaccagtga acgcggtttg catcacttgg tttacgaagt
      601 tatagataat tctatagatg aagcattagc aggttattgc gatgaaatta tttgcactat
      661 aaccagcgaa ggaaatatta gagtggaaga taacggacgc ggaattcctg ttgatatcca
      721 aaaagatatg ggaattccgg cagtgcaggt tgtcttaacg gttctgcatg cgggggggaa
      781 atttgacgat aaaagttata aagtagccgg aggtctgcat ggagttggtg tttcagtagt
      841 taatgctcta tcggaatttc tggaagtgaa tatttttaag gacgggaaag aatactttat
      901 gcgctttgaa agaggtcttc cggtaaccga attacaggtt ttaggggata ccaataaaac
      961 cggaacagta gtaactttcc gtcccgaccc gcttattttt gaaacaacgg aattcagttt
     1021 tgattattta acttcccgct taagagaact ggcattcctg aatagtggat tacgcattat
     1081 tctaaaagat gaacgcagtg gtcgggctca tgattttaaa tatgaggggg gaataaaaag
     1141 ttttgtggaa tacttaaatc agaacaaaaa accgcttttc cccaatccga tatacatttc
     1201 cagcgtgaaa gaaggaatgg aatttgaagt agctcttcaa tataacgagg gttatcagga
     1261 aaacatcttc agttttgcca ataatattaa tactactgaa gggggaactc atttaagcgg
     1321 atttaaaagc ggtttaacgc gtgccgtgaa tacctacatc aaaaatgccg atctgcttaa
     1381 aaatgaaaag gttagtcctt ccggtgaaga tattagagaa gggcttactg cggttatttc
     1441 acttaaaatt gctaatcccc aatttgaggg acagaccaaa accaaactgc aaaattcgga
     1501 agtggaagga attgttaata gtgctgttta cgaaaagctt atggagtatt ttgaagagca
     1561 tcctgctgaa gcaaaaaata ttacaatgaa aagtatttta gcagcacgca gtagagaagc
     1621 agccagaaag gcaagagaac ttaccaggag aaaaactgtc ctggaaagtg gctccctgcc
     1681 aggaaaactt gctgattgca ctttaaacga tccggaaaaa acagaactct ttttagtaga
     1741 aggtgactcg gcaggcggaa gcgccaaaca aggaagagat cgttcttttc aggcaatact
     1801 tcctttgtgg ggaaaaatgc tgaacacgga aaaagcaaga gtggataagg ttttgaacaa
     1861 cgagaaaatt caaccgatta ttttagcatt gggagctgga attggacagg aatttgacat
     1921 tagcaaactg cgttatcata aaatcattat aatggctgat gccgatgtag acggctctca
     1981 tattgccact ttgctgatga cttttttcta tcgctatatg cgtcccgtaa ttgaagaagg
     2041 taaactctat attgcaatgc cgcctctttt tcaggttcgc aaaggaaaac agaaggttta
     2101 tgtgtataat gaagaagaac gggataaagc aattcaagaa tttggtgaaa aaggtgtaat
     2161 tgttcaacgc tataaaggac ttggtgaaat gaatgcggaa cagctttggg aaaccactct
     2221 tgatcctaaa aaccggaaaa tgattgctat caaaatggat gatgccattg aggcggatag
     2281 aatgtttacc attttaatgg gcgatgaagt agaacccaga agggaattta ttcaggaaaa
     2341 tgctcgctat gtaaaaaatc ttgatgttta aggagcaaaa atagatgtct gaaacaacca
     2401 atattattcc tgttcaaata gaagaacaac taaaacaagc ataccttgat tattctatga
     2461 gtgtgattgt ttcacgcgcc ttacccgata ttagggatgg cttgaaaccc tctcaacgcc
     2521 gaataatata tgcgatgcat gaacttaatt taacccccgg aagtcacttt cgtaaatgtg
     2581 caaaaattgc cggggacaca agcggtaact atcatcctca cggagaacaa gttgtatatc
     2641 ctactttagt gcgtttggca cagccctgga taatgcgtta tccgttaatt gaaggtcagg
     2701 gaaatttcgg ttccatagat ggtgatccac ctgcagcaat gcgatataca gaagcacgcc
     2761 tgcaaaaaat aacaatggaa atgcttaccg aattggagaa ggataccgtt gaatttagaa
     2821 gcaactatga tgaaacccgt caagaaccta cagtttttcc tgcgcggttt cccaatttac
     2881 tggtaaacgg ttcttccgga atcgctgttg gaatggcaac caatatgcca ccccataata
     2941 taggtgaggt ctgtgatgcc attattgctc tgattgataa tcccgatttg caaccgctgg
     3001 agttgctgga atatattaag ggacccgatt ttccaactgg tggttttatt ttgggaactg
     3061 agggaataag agaatatttt gaaacgggta gaggcaaaat tattatgcgg ggtgaaactg
     3121 aaattgaaac cctacccaat gatttagaac agattataat tcgttccatt ccctatcagc
     3181 tttctacaag tcagcttata gaacggatgg tggagctggt gaaagaaaaa aaaattgaag
     3241 gcattagcga tattcgggat gaatccggac gcgatggaat gcgcattgta attcaggtaa
     3301 aacgcaatca tgatgctcaa gtagtgcgta atttgcttta taaatataca cagctgcaat
     3361 ccacttttgg cgtaattaat ctctgtctga tagatggtgt tccgcaagtt atgaatatga
     3421 aagaaatgtt gcagaatttc attgattttc ggcacgaagt tgttttacgc cgcacccaat
     3481 ttgaactgcg caatgccaaa gaacgattgc acattttaga gggcttgaaa atagctctgg
     3541 ataatctgga tgaggttatt gctctaatcc gtgcttcgca aacaccaaat gatgccagcg
     3601 ttggtttaca ggaaaaattc ggcttaagcg aaattcaggc aaaagctatt ctggatatgc
     3661 gtctccaacg cttaaccggt ttggaacgcg ataaaataga aacggaatat caggaattgt
     3721 tgattattat tgctcgtctt accgaacttg ttgaacataa agaacttaga atgaatttaa
     3781 ttaaggaaga aacagctgaa attagagata aatttgctga taaaaggtta actacaatta
     3841 tccctactcc agctggaagt ttcaatatgg aagacctgat tgcggatgaa atggtggttg
     3901 ttactatcac tcatgatggc tatgtaaaac gcttacctgt tgatacttac aaagttcaag
     3961 ccagaggagg aaagggttta agcgcttcca atctgaaaga agaggacttt attcaatacc
     4021 tcttcgttgc cagcactcat tcctatatct tgctttttac cgatcatgga atgtgttact
     4081 ggctaaaggt ttttgaaatt cctgaagcaa gtagaacagc cagaggaaaa gccattgtga
     4141 atttggtaaa acttggtgaa ggagatagaa ttaaagcttt tgtaactttg aaggatttcc
     4201 atcccgaaca aaatatcgtg atgtgcacgc gtaacggttt ggttaaaaaa actcctttaa
     4261 ctgctttttc gcatcctcgc tccatcggta ttaaagctat caaactgaga gaaggcgatg
     4321 aattgattga tgccagaatt tgtgaagcgg gagatgatat tcttctggct actcatgagg
     4381 gaaaatgcaa tcgtttcagt gaaagcgaaa tccgttctat gcgtcgtttt tcccaaggtg
     4441 tgaaaggaat tcgtttgcgc aatggagatt atgtaatatc tatgactatt gccactgctc
     4501 aggatcaatt ggaaaacggt aaacccaatt cctccactat tttagccatc agtgaaaagg
     4561 gttatggcaa acgaagccct atttccagtt attctgttac cagaagaggt tctatgggtg
     4621 ttataaccct gaagacaaat gaacgaaacg gttatttggc tgccttgatg ctggtgcggg
     4681 atgatgacga tttaatgatt atcactcaag gcggaatgat tatccgacaa cgcgtttccg
     4741 aaatttccgt ggtgggaaga aatactttgg gagtgcgttt aataaacctt gctcccaatg
     4801 atgtagtgcg cgatatatct cttgtccatt cagaaccgga tgaggaatcc ctggataaag
     4861 aagtggaaac cttaaaggca aaaccagctt ctaacctgga agaatttgca gatgtagaag
     4921 atgaagaatt cgctgaagaa gaaatggatg aattggaaga agaccttgaa aatgaagagg
     4981 ataacgaata aacattgcag aatgatgctg acagcaggaa ttttatttct gctgtccgct
     5041 tgttctgtaa gccagtttgt tcccggggaa ggagtcgtta gcgaaaataa ttatgctatc
     5101 attcgtgcgg atacattatt gattattgtt aaaccccaaa gctaccctgg aagttatcag
     5161 gaaatcaata accgtttctt ctccgtcttt atcagtataa aaaataattc cagacaaaaa
     5221 attaaactgc aggaaagctc attcagcatt ctttgcaatg aaaaacaata tgatcctgtt
     5281 cccgtagatt acattctctc tgatctggaa cgcagaatga tgttgaagga attcagtgat
     5341 cctttttctc ccacaacaga attcggtatc acagacagaa ataaagaaca agaaatgtat
     5401 tatgaactgg taaataatgc atttagctcg ggagaacttt tacccggcgc caaaaaagaa
     5461 ggatatctct tttacaatag agaaattgcc tctgcccgct attttacaat taatatcctg
     5521 ggaactgcta ttcccttcgt aaagcaataa tatacattct atattaataa tacataaaag
     5581 gacaaaataa ggtttagaag gttgagaagg tttatagggt ttatagggtt tagaaggttt
     5641 agagggttga gaaggtttag aaggtttaaa gggttgagag ggttgagaag gtttagaagg
     5701 tttaaagggt ttagagggtt tagagggttt agaaggttta gaaggtttag aaggttttag
     5761 agggtttaga aggtttagag ggtttagatt ccctttgccc tctgctaata agcccggagg
     5821 gcgaaacaat gatagcgatg gtggcgtaag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn tgccctctgc taataagccc ggagggcgaa acaatgatag cgatggtggc
     6001 gtaagcccct cggaaaaaga gaaataaacc ccccccttct aaatctaata ttttgattcc
     6061 gaaaaggatt atccggaagc taaaaatcag ttaagaggaa accatttatg atttatctat
     6121 aaaaaaatta tctaatatct ctttcgctca cgagtcctca ctacaaaaca aatttgaggg
     6181 taccttaaaa gctatttcag cagggacgac tcttaacttg aattataaat ataacttcaa
     6241 agtttccaca aggttaaaga gtatctttta tcctttcaat ggtcaatttc aaataatcaa
     6301 tattgatatc tattcctata taatttctat gatatatttt agcaacaaca ccagtagtac
     6361 cgcttccatt aaaaggatcg agaacaaggt ctccttcatc ggtagaagat aaaatgattc
     6421 tttccaataa tgctatgggc ttttgagttg gatgttttcc gtgtattttt tcccttgcag
     6481 gtgtagttgg aatacaccaa acacttctca ttggtttacc tttcttattg ataatatctc
     6541 ccatccagtc agtatttttc atcagctcat aatgatattt ttgtttctgt aaggggtctt
     6601 taattgccca aagaattgtt tcgtgagaag cagtaaaaca ttttctggta aggtttggag
     6661 ctgcattagg tttgaaccag caaatatcat taataattct aaacccttgt ttttgtaact
     6721 catatccaca ttgataaatt gaatggtagg ttccgcttat ccaaatagtt cctgatggtt
     6781 ttaatatttg tctacataca gaaatccatt tatggtggaa ttctgatttg ttattaaatt
     6841 catcaagcaa atcccagtca ccctttttca cacttaccat ttttccgctt ttacaagtta
     6901 tggttccatc cgataaaaag tagggaggat cggcaaaaat catatctatt gagttaggtt
     6961 caagttcttc taaaatttca atacaatttc ccagatataa gcagaaacct tcagcagaaa
     7021 aatatttact atagctcata atcaattagt aaggatatat tctaaaacac catccaaaac
     7081 catttttata ttcaggatat aatcaagctc attaaatgct tctttcaagg gtctttgcgt
     7141 tgttttccaa ccccaaccat cagttatcca tataaattgt aggttatgac ttttccaaaa
     7201 gttatagata tttttatatt cacctgctgt tgattttaat tttgaaccgc caacgctgta
     7261 gaaattgact tcaattaaat ataactgacg gtcttttttt ataatgaagt caactttacg
     7321 atttgtttta tccacaggaa ggtcaattcc ccattgtttt tctacttttg acttgttagc
     7381 agatgctaaa tattcaatgt tgtttttttc acaaatcttt ttaatatgct tattcattaa
     7441 ttgttccatt gcttttcctg ctctattttt gcgggcattg gaatccaatc ctacttcaat
     7501 tccgaaaaca taatcaacca agttctttat tttgtttgaa cttaataact gtaataaacc
     7561 agctctgtca gcaaaattta aggcatcttc atcagaaagg tttttaaaat tgaactcttt
     7621 tgcttggagg tcgttttcat cattagaagt taacagggta atactatctt ttctgcaagc
     7681 aacaagaaca ggtaaaatat gcttaatttc cggatgtttt ttcagaagat attgaaattc
     7741 attgtagata ttctttttgc ctatgagata gttcattgta tttaggcata tttcaatatc
     7801 ctttatgtgc ttggatactt tttcccagtc aacaagataa gaccagttat tgattgtagg
     7861 taacatagta tttattagat agttaaatac tttttcgggg ctgttcagtt ttattttttt
     7921 atataggggt aattgtttca aggtaaacct cccaatctat gataaacatt atatatacta
     7981 tataaagtat aatttttatt agtttagaac tgatacatac tccatcacct caataattcg
     8041 tcacaagtat ttcagtaata ttacctcttt tttcggcatc ggaatttatc attctttttg
     8101 cttgtattcg gcttatctta tactctttat agaggttatc aaaaaagtca tcctcgggat
     8161 ttgtaactct gggatcagag tttgacaaaa tctgatatac atcaggtcta tccatatcct
     8221 taaaaaagtt agctaatctg atttggtctt tatcggcaaa accttctttg gcataagaat
     8281 tgaaattgga ggtcttggtt aatggtctat agggaggatc ataataaatt aaggtgtttt
     8341 cttcaacata atttgcagcc aaggaaaagt caccctgtag aatttctgta ttagacagtg
     8401 ctttatttgc atcccaaaga ttttctgcct gacatattga gggtttatca taacgaccat
     8461 gagggacatt aaattctcca tttttattct ctctatataa accgttaaaa caagtcctat
     8521 taagaaaaat catataggca gtgtgtctta tccataattc gttgtaagtg gtatcaaggt
     8581 gctttttact ataattaaac ctttttctga tatcataata caaagtagtt ctctcagttt
     8641 gggaagcatt attatattta ttatccaatt cggataataa agcaattaaa ttctctacag
     8701 catatttaat aacttgatag gttataatca aatcacggtt gatatctatt aatattgatt
     8761 tatctatctg aaaacaggat tgtaaataaa agaaaaatgc accacctcca ataaatggtt
     8821 caatataaac aggaatatgc atactttttt tgaatttatt cggaagccga tcaacaagtt
     8881 cgggaattag ttgtccttta ccacctgccc attttaaaaa gggttttgca tcaaagttaa
     8941 acaatttcat ctgagtggta ggcatatatt ccttaaggtt tactgcagat aatatctggt
     9001 ctttcataat atcttcttcc taattatttt tactttcttg tcattagccc ttactgccaa
     9061 acaaatttga gggtagcgta aaagctattt cggcagtgac gacttctgac tctttgctct
     9121 cagccctaag ctctcagccc tctgctttta ccggtattca tagagcatcc aaccctcttt
     9181 tcccagtcct aaaaaaatgt aatcattggc tacatccaaa tcgtaaacgg gaatagtgag
     9241 aaagatgttg ttgatatctt ctgtcttgag gggattagtt acatcaataa ttttaactcc
     9301 cccgcgtcct aaggcaataa aaagacggtt atctttaata tccaaactca ggatttcgtt
     9361 agtcattcta atttggctga tgagagttgg ctttttgggt tcataaacat tgaaagccaa
     9421 gagatttttt ccggcaccga tccaaattgt atttccgttt ttaaccaaac aagtggcatc
     9481 tataatttcg taatttacac ccaaagaaac aggataggat tgatcggtaa tatctacagc
     9541 aactaaacca aaatcattga gagcaaaaat ataagggtaa cttgccacaa agtccttaat
     9601 gccccatttt tcagaaaaag tagctaataa ttcgcgtgtg cttccaccgc ttaaattaat
     9661 aatatcaatt cctctataac ggtctgccac atacaggata gaaccttcgc gggtaagttt
     9721 atcacctaca attgtttgag ggaagaaaat gcgggaacca gtatataagc tatctatcag
     9781 aataacctga ttggtaggtt cattggaagc tacataaaga tgttttccgg caagagtttc
     9841 aatatcttca atcggattgg gcgaaaaaaa ggcagcttcc agtttgggct gccaggcatt
     9901 aaatatggaa taaacccaaa tgtaattctg gttattagta taaaccaaat catcgtcaaa
     9961 gcggatggaa tatgcctgcg aactcttaac ctgaaccagt tgatgcagaa atttattatc
    10021 aatggcaaag agggatatac acattagcag gcaaagaaaa aagaatataa agcgtttcat
    10081 ttttgctcca gtaaccagtt aatgatttgt ttggagttaa ttccttccgc ttgagctgcg
    10141 aaggagggta aaatacgatg ctgtaacaca ttgatggcag atgccttaac atcttcttct
    10201 gcaggagtta aacgtccatc taaagcagct cttgctttgg cagaaagaat aagatattga
    10261 gaagcacgtg gtcctgctcc ccaggcaacc cactgtttaa tttcatttaa ggcatcatcc
    10321 gtattgggac gggattttct tactaaattt acggcaaact taagaacatg atcacttaca
    10381 ggaagagaac gcactgcttg ttgtaaagat attatttgtt cacccgtcag ttgaggtgta
    10441 attacaggat tttccagtcc cgtagtgctt tcggcaattt ggagttcttc ttctaaagta
    10501 gggtaatcaa tatttataaa gaacataaaa cggtctaact gtgcttccgg aaggggataa
    10561 gttccctcct gttcaatggg attttgagtt gccatcacta aaaaaggttt acttaaggga
    10621 cgggtaacgg ttccgctggt tactgcatat tcttgcattg cctgtaataa agctgcctga
    10681 gttttaggag gtgtgcggtt aatttcatct gccagaatga tattggcaaa aataggacct
    10741 ttgatgtatt caaagtgctt tttttcggta acggggtcat ttaccaaaat ctccgttccg
    10801 gttatatcgg aaggcattaa atcgggggta aattgaatcc gactgaaaga aagtgaaagt
    10861 gcttttgcca gagaagaaat aagcaaggtt tttgctaatc ccggaacacc ttccagaaga
    10921 atatgtccat tggcaaaaag ggcaatgagg acatcggtta gaactctgtc ctgaccaatt
    10981 ataacattat gtatttcgtt cagcagggtt tctttcaagc tctgcattgc agttatatct
    11041 tgtatcagtt cttctttagt catttatttt atctccttta atagctgtga ataagagttc
    11101 aggaccaaga accgcgaaag caattatacc ccataaactc attaatccgg cagttagaat
    11161 atgaccaaaa gccataattg ccgaggcagt ggtctttgtc aatccaaatc ctatactgaa
    11221 aataataatc atcatccatt cgttactgcc aagttgagcg ggaggttgag gaatggcata
    11281 ggaaagattt attaaagtat aaccaaataa aaccagagca aaaggatata atattccaaa
    11341 agctcggaac agcaaataaa aatatattcc atccaataaa actcctccgg cagttaaaaa
    11401 taatgccgaa accagcttga aaggatgatg ttcaaagagg ttaagctcac tgataaacat
    11461 ttctatagcc gggtttattt ttgcctttgc tttttggggc aaccaggaaa aaagcgagga
    11521 aataattttc accgccaatt cctttttcca aacagaaata agcagtatag aaaaagctac
    11581 tataaagatc agcaataata aacaaagtaa aatgaccatt ggagtgctca gttcaacggt
    11641 tgtaaaaggt aaaacaatga tgataaacaa gatggcaata gtatcaaaga ccttatctac
    11701 aaataccgaa ggcaatactt tcatataggg tagagaataa ttttttttaa tgaaccaggc
    11761 acgggcaaaa tctcctgctc tgataggaat taaatagttg agcagatttc ccgccatagc
    11821 atacagccag gttttgaata tacctggatt agcaggagcg ggaataagta aacgccaacg
    11881 caaagaacgc aaaaaatagg cagaaaggta tgccacggaa gcaattataa cccaattata
    11941 attgacttgt gcaatttggg atttaatttc ctctaaagga gtataatata accaggcagc
    12001 aagcaaaagg atgcctataa ttagcccgat tactaatgaa ttacgatttt ttcgggtcat
    12061 tcactatttt ccggagtgaa aagcagataa taatggtgag cccaatattt tttgaaccgt
    12121 ttgggagcag ttctttccac aaaactatat ttaaacatct tttcggcaaa ttcactgtct
    12181 tcatcctgat aataagtaag aatggtaaca ggttctgtat tgttttcctg aatttcctgt
    12241 tttttcagca ggcggttaaa aaagagttct tttttcattc caatatcggg ccagggggga
    12301 caatcaatat agttttcgtc ttgcaattgc catttatttt gtttcatcag gaatttgatg
    12361 gtggcgggat caagatattc cggatgcaaa aaagggtatt tctccggtga ataatccttt
    12421 atctgcattt tatagccgat tccgtttcgg ttgggaacac aaattagaat tgcctttttg
    12481 caaactctgc agaattcgga aagaaaactt ctaatgtttt gagtaaacca caaagcggaa
    12541 aaattgaagc ccaaatcaaa atgtttgtca ggataatcca gtttcacata atcaaggtta
    12601 tatttgatat cggtatgtaa acgcaattct tgccacaatt cccgaatttt ttctatgcgt
    12661 tctaaattat gatcttccag agagaccttt attccctgtc ttgccaaatt tagcagattt
    12721 ataccgctta ctccggtaaa accaaaacag ggtgtttcca gagccgattg aattttatag
    12781 gtcttacaaa caaaatccag caacttattg aggacaatgc gttcataaga agagcccaaa
    12841 ccctcatccg gattatcaaa atattttgcc cagtcattaa taatcggaac agccatcagc
    12901 gtttctgata gtcctttatg gttatttcaa attcgggaat ggtgaaatgg gatttgtaac
    12961 cttctttttc cattatgtcg taataggcgc tcacatcgta aaaccagctt ttggaaatta
    13021 gctcaaaacg gctgatccat aattcattct tcacgagacg agcaagtttt tcaccccagg
    13081 caaagaaaat tctatcaaat cttaaagcac ggggatagtt ttttccgtga agctgacggg
    13141 aaataaaatc taccaatgat tgtaaacgca ccggttcacg atctgctacc gtaagcgtaa
    13201 gtccactttt ccattcgtgg gttgcaagat agagaaatgc tttcaccaaa gcatcaatat
    13261 gacaaagatg aatccaaata ggtctgttta taagcgggaa aagatgtttg tctactaatt
    13321 ttaccatttg ataagggaaa ccgaaatcgc cttctccata agcaatactg ggacggacaa
    13381 ttcctgcatt cagtccattt aaaatagcgc gattgatgat gcgttccgat tctattttcg
    13441 tgtaatgata ataattatca gggtttttct cagtttgcgg accggcaggt agttcctgag
    13501 gaatagtacc aaaaacacct acggaagaac aataaatcaa acgggcatct ttggcaaggc
    13561 aatattctac catctgtcca gtgctgtaaa cattactgcg gagatatgtt tcttggggan
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tctaccatct gtccagtgct
    13741 gtaaacatta ctgcggagat atgtttcttg gggatatttt ctgccaccgc gtaatgcacc
    13801 aatatgcaga atggtatcaa attcattcgt aaagagaaat tcctttaatg aagcagtatc
    13861 ggcaaggtca atagctacaa gacgaattgc cttttcaaat tcctgatagc gccttggatc
    13921 tgtcttagga cgtactaaag ctgttatatt aaaatcggga caggacttta acatttcccg
    13981 caaaacattt ctaccaataa aaccggtaat tccggtaatt aaaattttag ccaaaaatcc
    14041 tctttattaa actgcgtctg tggtgatgat gagaatgatg aggtcggtaa acctgaacat
    14101 attcataagg gatattttct ccgctggcaa tgcaggaagg gaaaacataa aaaagcaaat
    14161 gcgcggtatg attatcaatc atctcttcta ccttatcgcg cgcttcaaaa taggcaagat
    14221 atttaccccg aatatgatcg tccgaagcaa taaaatgtgt ccaaccctta tttactaaaa
    14281 tccaggcagt ggacttaacc ttctcgccat aaagtccaag caagcttccg gcattggttt
    14341 gcatataaat attacgataa atcaggtctt cagcgcgaga gggtttttgc ataatgctca
    14401 catagcgttc cgcatgagcc aaaataggtt tgtaaccagc acgtaatagg ggaaagatat
    14461 tattataaaa atcactgggc agaccattta agtcgctttc caaaaggata tacgaacttt
    14521 tacccatagt cagattgtgg gtttttatgt cttccaaaat atcgggttgc aaataaatct
    14581 caaaaccggg ttgaatatca aggtctatac ctgcatcttt tactttatta acgatagtat
    14641 tgaatttttc atcatattcc tgacgactgt attgataatg acctttaaag taatgtgccg
    14701 ttaaataaac tcttttaatt ccaccttctg ccatctggca taactgactg agagaatcat
    14761 ttagggattt tgaaccatcg tcaatattag gcagcaggtg agtatgaata tcaaccattg
    14821 atcagggtaa tctctctttt atcatagtta cgaattccaa taaaacagag ttattaggat
    14881 agggcttcag cattttagct gaatcggcaa gcatattttc tactgtttta cgagctttat
    14941 caaaacccag cagagctgta taactggctt tggaaacagg aacatattct tcatccaaac
    15001 cctcacttcc gcgagaataa tccgcttcaa tatcttcaat catttgatag gcaattccca
    15061 aattcaaggc ataagctctc ataacctgat gtgtttcttc atctgcacta cttagcaaac
    15121 aggcaatatc ggcagctgcc tgtaataacg atccgacctt tttcatatca atataattca
    15181 aggtgtttat tttcatcact ttatgccggc tggaaagagc tactacctgt cctccaatca
    15241 ttccataact ttttgtggca ttggatagaa tgtgaattgc ttccagggat tgttccgggt
    15301 ttttaagctc gctgataatt tcataagcaa gagaataaag tgcatcagca gccaaaatag
    15361 ctatggcatt accatatttt atatgcaatg ccggttgacc tcttcgcttt gttctattca
    15421 tcacacttgg caaatcatcg tgaactatgg ctccattatg taacaattca atagcgactg
    15481 aggactttag agcatccgga aaagcgcgat gtttcttaaa tcccgtaagc atttcaaaca
    15541 tagaaagaag cagtaaagga cgccatcttt tacctccggg aattactgct tcataaattg
    15601 cttttttaag ctctgtggca tatttgtctt caaatgttag tgcgttttta agttcctggt
    15661 caataagttc aaaacgcgtc tcaaaatact tctgtaaaag caaagcatac tcccttttca
    15721 tcaagcttga caaaagctgc tatttatctt cttctttttt ataaaatacc atagttttgt
    15781 caaggttttt ctctatttta caccgaaatc accgaaaaca ctgagaacac caaaaaaagt
    15841 gtagctggag tacaccgttc ctccgcatac aaaaaactga attttataag caacattata
    15901 gcgataataa gtgactcttt ggttttgatt ttgatttatt ttctttctcc tggatcatta
    15961 gttcgcgtgt taactttttt caggatgact cactcaaccc tataaacctt ctaaaccttt
    16021 atgcaaggga cttacatacc cttatacatc attgtgtcgc cctttggggc ttttgggaac
    16081 cgggaaaggt aaataaaaga aaaataatcg gggcttactt tttccgaggg gcttacgcca
    16141 ccatcgctat cgttgtgtcg ccctccgggc ttattagcag agggcaaagg gcagagagaa
    16201 aataggcctg ggatgaacag gatgatttgg gcagagggca gagggcttag ggcttagggc
    16261 agagagaaaa taggtatggg atgaacagga ttatatggat aaaaaaatag ttccgtagga
    16321 acgacagata ttaacgacgg gttttaaccc ggtgaaaaga agacagagtg aaaataagtt
    16381 ccataggaat gacagctatt atagaagggt ttcaacccgg cgagtaagag gagaatgaaa
    16441 aaaagggctc acgcagattt tatttgtgtt tggcagcgag gactaagaac agaaaaggac
    16501 aaaggaagta tgatatgcag aagtataact atatggaaat agtgtacatt atatttaact
    16561 cctgcacccc ggcactccgg cactccagca ctccagcact gtatttgttt tttggattat
    16621 actgattatt tatattagta taaattgtaa aaaaggatga tgctatgcgt aaataccgtt
    16681 taattctgtg tctgctgtta ataataattg agctatcagc atttcaggta tatttaccgg
    16741 aaggtcaggt aaaggaaatt accaaagagg atttaagcaa agaaaaactc gtttctttca
    16801 ctactatcag agataaagat ggaggacaaa aagaagaaac ctggcaaggt attcctttaa
    16861 ttccctggct caattcttta acgagcagtc cctggcaaaa tttgcaaatt gaaagtaatg
    16921 acaattttat gctaactttt caccgtttag aattggatga caataatgct tatctggctt
    16981 tgaaattggg tgcagaagag ctttctgaat ataatatccg tttaattttt acccgctacc
    17041 gggaaaatgc ctggatgaga aatatcagat gcttgcgttt gctgaatttt atacctgtgc
    17101 ccaaacccaa acaaatatac ttttgggaga agggtttgca gaaaaacggc attgatctaa
    17161 aatccggagc aattgcaatg aaggacttaa tgcaaaaagg ttttagcttg gaaagcgggg
    17221 atcttatctt tgtggatgaa caatttcgga agttatgttt gaattatcct cgtgaaatag
    17281 ccgatgcgtc tctatttatg gattcccggg gacaatattt tctggtgccg gaatcttctt
    17341 ccgctctcaa aatctctttt gaaaacgacc tttcccgaat tatctatatc cagtgtggag
    17401 caattgctta tcttgctgaa gacgctatag aaaatatgga aaaaatagcc cgaactcttg
    17461 aatgggactg gcagggggat tatttatact tgattaaagc cgagaaacag aaaatcaaag
    17521 caggtgaaaa accgatggat atcaatgcgg actgctggct ggaattttag agagaactat
    17581 ctcacgcaga ttgcttagct tttattgtta caccgaaaac accgaaaaaa ctgagaaaag
    17641 attattatct caacttacca tttaactcca gcaccccagc accccagcac tctaaaacat
    17701 aatatattgt tccaccagca taaacatcac tttatcacaa a
//
LOCUS       DAOOZV010000037        17416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3343_length_17416_cov_59.421174, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000037 DAOOZV010000000
VERSION     DAOOZV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17416
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3343_length_17416_cov_59.421174"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..929)
                     /locus_tag="PLW08_05040"
     CDS             complement(33..929)
                     /locus_tag="PLW08_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPL52004.1"
                     /translation="MDLSSINNFRKSRAGKIILYFLKLGLSIFILWMIFRKVNFISAL
                     KDIISLPLGIVLLLLVISFIRHTLQFHNWYFSLQINNLFKAPKKEVISSYLIGQTLRF
                     AIPGGPASFAKIFYVPNSSKISSFWSSIAEKSFMTWTTWFFALLAGMLYYRQSPLWIW
                     IILLLILIFLPFLVKLIFGASKKFSSLQALYSVQFPKIISVQVLSNLLCFVQYWLILN
                     CFLPINFWQSWIRMALTQFSNTIPITISGLGLREGFAIHFLAGAGFTAEQAVSVSLTL
                     FFIHDVLPALIGIFYLLKSKKV"
     gene            complement(933..2390)
                     /locus_tag="PLW08_05045"
     CDS             complement(933..2390)
                     /locus_tag="PLW08_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPL52005.1"
                     /translation="MKELSADLSNLAEVFSALFPPEKKSSAQTFFLKQLSLKMHSYYQ
                     GKMQTLPKSGIWGFNWFNVWYTPGVSSVSTAIRDNIAKSYELTNRGNLVAVVSDSTRV
                     LGDGDCGIAGGLGVMEGKAMLMKYLGACDAISLCIDSRAENGKPQAQKIIDFVKMLQP
                     SVGAVNLEDISQPNCYLVLDELRETCSIPVWHDDAQGTACVTLAGLINALKLADKEIH
                     NIKCVLYGAGASNTTIAGFLLQSGLNPERLIIFDSKGSLHPGREDLKSNPDKFRQWSL
                     AQNSNPECVEGIENALQNADVLIALSTPGPGTIKPEWISKMAKKSIVFTCANPVPEIY
                     PYNAQKAGAFIVATGRGDFPNQINNSCGFPGILKGVLTVQASKITDTMAIAAAHSLAE
                     YAEKRGIHPENIVPQMDEEDVFAYEAAAVARQAITEGLAGLKFTYEEVFKQVQKEINA
                     TRALCQEMMENGYIAPPPRNLIAETLQQTIVRFQN"
     gene            2883..4436
                     /locus_tag="PLW08_05050"
     CDS             2883..4436
                     /locus_tag="PLW08_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPL52006.1"
                     /translation="MQSTKEAIQGLLEKEKKILQMGGEKAIAKQKQGGKLTARERLDL
                     LFDEGTFRELDMFVSHRCDNFGMEKIEIPSDGVITGHGLVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKIMDMALKSGVPLIGIQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRAVTGEETTQEDLGGAM
                     AHNSKSGNAHFACENDADAIQKIKVLLSYLPSNNMEEPPRIKTNDDPWRLCPELDSII
                     PDDPKMSYDMRDVIHSVVDDGIFFEPHYYYAQNVIIGFARLNGRTIGIVASQPTILAG
                     CLDIDASDKATRFVRFCDSFNIPLVTFVDVPGYLPGTHQEWNGIIRHGAKLLWCYSEA
                     TVPKLTVVTRKDYGGSYIAMSSRHLGADMVFAWPTAEIAVMGAQGAANIIFRKEIAAA
                     EDQEAKRQEMIQIYEQEFNNPYVAATRGYVDAVIVPAETRKRLIDALEILCTKSESLP
                     PKKHGNIPV"
     gene            4456..4644
                     /locus_tag="PLW08_05055"
     CDS             4456..4644
                     /locus_tag="PLW08_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52007.1"
                     /translation="MKDNKEIAAIGAVIALIQSEETSITYKEPVRGTPSQPYTAYGRE
                     LTMHYRDLTQRRIIKRCR"
     gene            4658..6643
                     /locus_tag="PLW08_05060"
     CDS             4658..6643
                     /locus_tag="PLW08_05060"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPL52008.1"
                     /translation="MDKHSCVEMTTMHYEPDRPKAENPVKVQDLTFRDGHQSLFATRG
                     RTEDMLNVAELMDQVGFYSMEIWGGATFDTMHRYLGEDPWERIRVLKKHITKTPLSML
                     LRGQNLVGYQNYADDVVEAFVQRCCDNGIDIFRVFDALNDFRNFITAVKIIKKNNKLF
                     EGAICFSLTEQRMGGDIYNIEYYVAKAKQLEEMGADTICIKDMAGLIAPYDAYELIKA
                     LKENVKVPVHLHTHFTSGMGDLALFKAIEAGVDIIDTCVAPYAYRTSHPAIEPLVISL
                     LGTNRDTGFDIKLLNKIGKEMEKDIPKYLEFADTTKFSIIDTDVIIHQTPGGMLSNLV
                     NQLRQMDALDKLEDVFKEIPKVRKDLGQIPLVTPTSQIVGIQAVNNALFDSYEGEYSR
                     ITEQVKDLCYGLYGKTTLPINPEVQKKALKGYPRGETPITCRPGDVIPPAMEQVHKEC
                     EGLAKNIDDELIVALYNITGKRFLRIKYGLEPMPEEMKPKTLADVKKEQELIAKAKAG
                     KLIEKPEPKDKPQDLRQFDVFVDNTYYLVEVSEKGGTPKIVNSRPAPVISETIVKPAN
                     NPNPQVKPSNPAPEPKPAPVVNSEGGTKITAPMPGMFIRYEKKPGDPVKQGETVLVLE
                     AMKMYNNIPSPVDGVLVEAPFTGGANVAKGDVLAVIK"
     gene            6968..7504
                     /gene="pyrR"
                     /locus_tag="PLW08_05065"
     CDS             6968..7504
                     /gene="pyrR"
                     /locus_tag="PLW08_05065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPL52009.1"
                     /translation="MLVKSQIMDKDQMERSLQRMAHEIIEQNRGLEKIRLVGIRSRGV
                     PLAERLSSYLKLISNQEVPVGILDITLYRDDLSTISHQPVIKGSAIDFDIEDAVVVLV
                     DDVLYTGRTVRAAIDALMDFGRPKQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEII
                     KVSLEETDGEDSVKIVLV"
     gene            7470..8285
                     /gene="pyrF"
                     /locus_tag="PLW08_05070"
     CDS             7470..8285
                     /gene="pyrF"
                     /locus_tag="PLW08_05070"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPL52010.1"
                     /translation="MAKIASKSFWYKYQERYQAIKSVLCVGLDSDFSQLPEGVKNSEN
                     PIWEFNKAIIEATLDYACAYKPNLAFYLADGIRGLDALYKTVEFIPDNLPVILDCKIG
                     DIGNTMNAYLESFFTDLKVDAITLNPLMGSDVMQPVQDRNAFAFVLCLTSNPSAYDFL
                     QEGMAEKIANWLQDYPKENFGAVVGATHSANIKTMRNLLKDRILLIPGIGAQGGDLKT
                     VLQEAKDSSETPNILVNSSRGIIFASKGKDFATAAREEARKLTQEMKNALSKG"
     gene            8509..9750
                     /locus_tag="PLW08_05075"
     CDS             8509..9750
                     /locus_tag="PLW08_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52011.1"
                     /translation="MKKIFIFTLLISAVFCFAFQVDTFGPTADDFSCANLDQVPASFG
                     IQIANQKKLCLWENGIWNSYSYFTTGLTIKGTYMLNADTMLVAMGIYSYSDGIYNFDI
                     NTHNWQLNDWFILPNFVKYNPHNQLYYVGERDGLFYSANGHNWTRITSLGSNKCNSIA
                     WYDSNIITNNGQMVYYSNDNGLNWQQSSISNLSSFRFDDNGILYAIMDVGSDSDGLWR
                     SNDFGANWTNILYTSALSCLGPIYNNNVTLGWRESYADSCFVGMLNSSGEVTFFNHPL
                     LNAPVKQIDIFPYINTPSFYVLNSGGCFFVTGFLVGIDDPVIPQIPQAKISTYPNPAL
                     SYADIYLSKVSPEPCKLTIYNLKGQKVRNLDLAETLKEQTLSWDLKNNNGFKVSAGIY
                     FLVLQDTKGNCLAKTKLAVIK"
     gene            complement(10008..10733)
                     /locus_tag="PLW08_05080"
     CDS             complement(10008..10733)
                     /locus_tag="PLW08_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPL52012.1"
                     /translation="MQFDVSYFCFDGNSGKKNQDRVLVNKDLLTEGINHLQNLDNLFC
                     FVADGIGSLANSENAAQFVLEQLKYLPLKAEQNWQEIIDNFLQDINYQLVLWNRQQGD
                     FFDSATTLCGLIYQEGKFLTVNAGDSEIWLYREKYLQRLNKLNVLWEDIPNSPITNYF
                     GTDYPNLKLDFSSAVSSLNPGDKIVVTTDGLLKAISKKELIQILEEDSPLYIKIDSLY
                     HKLCAKYNPDNLGAIFISEKSNG"
     gene            10969..12747
                     /locus_tag="PLW08_05085"
     CDS             10969..12747
                     /locus_tag="PLW08_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPL52013.1"
                     /translation="MPGINCLINAQGITQQTQNTIDNYLQRLEATFPHLQFTKLMPCR
                     EVLISISQHRGYRVYTEKYRGWQIFHELQDNSFLTQAFQDILEQAEISQNPLSNMQNR
                     WNSELKEINNSFFLFAVNPELHKIILANDALARLPVYYTKQKEYFLLGRDISFVKAIS
                     NQLTLDPLFLALYLSLTYVPGRGTFYREIDTLAGGTLGVYDWLTDELQISSQQELRFI
                     EPLFGRSHKKWLAELVETFTAICVSYHTELPKVVSLSGGMDSRCVAGALQSKNIDFTA
                     ISFLDANQDAQDDVLIAVQTAELLKKTHRVLQLSPCAPEHYEKLFSLKAGLNYLSVAM
                     FLQYLEMILALYPESALFLTGDGGDKVMRYLLPDKQLTDEKQWLNYWYNQNAVFSPKA
                     SAELFGIQQKAMEEYLLNHISTYPTKDYNYKYAFALLAERSARWAFEGEDRNRYYFRS
                     ETPFLDYQFYRLAMQIPMEQKKDNLLYYSFLSALSPALAKLRYAHHQWSPAKMRNPFY
                     RYLVNKTRNFRIQYRKPKGKINSQPKFAEQEYLITRILKQSENPLLQALMPGAKNILT
                     LPDLQNLSSNQLGTLYTCVSVITGIV"
     gene            complement(13237..15099)
                     /locus_tag="PLW08_05090"
     CDS             complement(13237..15099)
                     /locus_tag="PLW08_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPL52014.1"
                     /translation="MRKTILVALLLLLAFSLFAKEWNQYYFRFELADKTTLPEISNII
                     SIDNVRGNWVYAYANDEEYSKFRELGLKTQLLPSPASLIEPVMSSDVKQTKLWDSYPT
                     YDSYVNTMNSFAANYPNVCQIVNAGTTVNGRSLLFAKISDNVNIAEAEPEVMYTSTIH
                     GDETTGFVLMLRLIDTLLSSYGTDQRLTNLINNLEIWICPNTNPDGTYYGGNNSVSGA
                     RRYNYNGYDLNRNFPDPNGNQYSGQPLQQETTLMMNLANNHHFVYSVNFHGGAEVVNY
                     PWDYTYIAHPDENWYISTSFVYANNAIANGPSGYFTSVSSNGITNGADWYVITGGRQD
                     WMNYSAHCREVTIEISNTKMPSASTLPGYWNYNYEAMISYLEQAMYGIHGIVQDPYGN
                     PLSATITVNGYDNSYSTVITDPAKGDFYRYLSPGTYNLTISASGFPDKTISGVVVNAN
                     TATSISVTMGELPHYQQITLTPGWNLLSFNVDLGTNNFSSVFGSNLLQIKDTAKSYAP
                     FMPSYFNTLSSLQSAKGYWVNNSSAQNLSIQGQLLNTSNYPIALNRGWNLIPYLPDNS
                     LPVASAIASILTKTQEVRYLSSVWNPVSGGTLSVLEPGKAYWIRVSEPCQLLYP"
     gene            complement(15100..>15747)
                     /locus_tag="PLW08_05095"
     CDS             complement(15100..>15747)
                     /locus_tag="PLW08_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gingipain R"
                     /protein_id="HPL52015.1"
                     /translation="SENITFGVSDGELTAYDNVIVIVNPVNVPDWQPIVYPNNPATVY
                     AQVSIEGIPAQLNDLVGAFVNNECRGTGEIVLIDRATAYSTILVNLASDGELVQFKIY
                     SYANDAVYPVQETLTMQTGSVYGSAENPVVLNGTFNIVLTPPQVNLVSHQNTYRLTWN
                     AVPNANNYRIYSCSEPYGTYQLVHTTANLYWEIPTTQQKCFFKVVAEQIGISKGK"
     assembly_gap    15748..15847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15848..>17416)
                     /locus_tag="PLW08_05100"
     CDS             complement(<15848..>17416)
                     /locus_tag="PLW08_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPL52016.1"
                     /translation="PINVYYQSLHGQSVYTKAELNNAGVFGPVYISQVGFNVTGVPNK
                     TMPNFVVRMKHTTATDVSSWIDNTNLVTVYTNASYTISTTGYNMLTLSTPFLWNGTDN
                     LLIDTAFGLIGSWSSTGTVQYTSMANGYRFTRNDSADQTNVFSSGSTSVYKPNVKLFL
                     TAVAGNPQISVNPTSFTFGSVAVGTTSTQNFTITNTGGSTLTGSIITPTGYSVTAAAK
                     EIRNTLSFSIPAGQSKTYILSFSPVSAISYNGNVTISSNSQTQANLSLPVTGSGYLPP
                     TISVNTNTLSATLTTGAESTQTFTISNTGSQSLTYTMVVSEPTGRNEVIIPAKTTGSK
                     SIAGSTLTLNADEYTSGTTVDWTFTVTNASTDTEWLKEVIVTFPAGVTVNSATNFVGG
                     SGGDLIPDLTTGTGITISWFGTGSNGWGVIHGSESATATVNVTISAGLVTTIVLPYTI
                     NGDIWGAEPNTVSGEISLACSLPPVEWFSVTPNSGTITAGGNQTISGHFSAVGMAEGI
                     YNAILTIQSNDPVHP"
BASE COUNT         5425 a         3290 c         3466 g         5135 t
ORIGIN      
        1 agtggttgac gaagacatca acttttagtt ttctaaactt tcttgctttt caagagataa
       61 aatataccga ttaacgccgg taaaacatca tgaatgaaaa ataaagttaa agataccgat
      121 accgcttgct ctgcagtaaa acctgctcct gccaaaaaat gaatggcaaa accctctcgt
      181 aaacccaaac cggaaatggt aatgggaata gtattggaaa attgagttaa agccattcgt
      241 atccaactct gccaaaagtt tataggcaaa aaacaattta aaatcaacca atattggaca
      301 aaacagagca gattgcttaa cacctgaacg gatattattt tgggaaattg aacagaataa
      361 agtgcttgca aggaagaaaa ttttttgctt gctccgaaaa taagctttac cagaaaaggc
      421 agaaaaataa ggataagcag aagaattatc catatccata aaggactttg ccggtaatat
      481 agcattcctg ccagaagagc aaaaaaccag gttgtccagg tcataaaact cttttccgct
      541 attgaactcc aaaaagaaga tatcttgctg ctattgggaa cataaaaaat tttggcaaaa
      601 ctggcaggac ctcccggaat tgcaaaacgc aaggtctgac caatcaggta ggaactgata
      661 acctcttttt taggcgcctt aaataaatta tttatctgca aagaaaaata ccagttatga
      721 aattgcaaag tatgccgaat aaaacttata acaagcagga ggagaactat acctaacggc
      781 aaggaaatta tatcttttag ggcacttata aaatttactt tgcggaatat catccagaga
      841 ataaagatgc ttaatcccag cttaagaaaa tataaaataa tcttgcctgc tcggctcttc
      901 cgaaaattat ttatactgct taaatccata gttcaatttt ggaaccggac aattgtttgc
      961 tgtaatgttt ctgcaatcag atttcttgga ggaggtgcaa tatagccatt ttccatcatt
     1021 tcctgacaaa gagcgcgagt tgcatttatt tctttttgca cctgcttaaa aacctcttca
     1081 taagtaaatt taagtcctgc caatccttcc gtaattgcct gcctggctac agctgcagct
     1141 tcataagcaa aaacatcttc ctcatccatt tgcggaacaa tattttccgg atggatgcct
     1201 cttttttccg catattcagc taaagaatga gctgcggcaa tagccattgt atccgttatt
     1261 ttgcttgcct gcacggttaa aacacctttc aggataccgg gaaaaccaca ggaattattt
     1321 atttgattgg gaaaatcccc tctccctgta gcaacaataa aagcgcctgc tttttgggca
     1381 ttgtagggat aaatttccgg aaccggattg gcacaggtga aaacaatgga cttttttgcc
     1441 attttgctta tccattccgg ctttattgtt cccggacctg gagtagaaag cgcaattaaa
     1501 acatccgcat tttgcagagc gttttctata ccttcaacgc attcgggatt gctattttga
     1561 gctaaagacc actgccgaaa tttatcagga ttgcttttca ggtcttctcg cccagggtga
     1621 agagaacctt tgctatcaaa aataataagc cgttcaggat ttaagccact ctgcaaaaga
     1681 aaacccgcaa tagtagtatt ggaagcacct gctccgtaaa gtacacattt tatgttatgt
     1741 atttccttat ctgccaattt taaggcattg ataagtccgg ctaaagttac acaggcagtt
     1801 ccctgagcat catcatgcca aacaggaatg gaacaggtct cacgcagctc atctaaaaca
     1861 agataacaat tcggctggga aatatcttcc agattaacag ctcccacaga gggctggagc
     1921 atttttacaa aatctataat tttctgtgcc tggggcttgc cattttccgc gcgactatca
     1981 atacataaag aaatggcatc acaagcacct aaatatttca ttaacattgc cttaccttcc
     2041 ataacaccta agccgccggc aattccacaa tcaccatctc ctaaaacccg ggtagaatca
     2101 cttacaacag ctaccaaatt acctctattc gtcaattcgt aggatttggc tatattatct
     2161 ctgatggctg ttgaaacaga agaaaccccg ggtgtatacc aaacattaaa ccagttgaaa
     2221 ccccaaattc ctgacttagg taaggtttgc atttttccct gataatagga atgcattttc
     2281 agggaaagct gttttaaaaa gaaggtctga gcagaggact ttttttctgg tggaaagaga
     2341 gcagaaaaaa cctctgccag attggaaagg tctgctgata attctttcat cttttatcct
     2401 ttgtgccaac aaggtaaagt gattgctttt gggagacaga tttttgtcaa gcaggaatga
     2461 agaattgagc tttggctttt cggaataaat gacatcctcg tcatttttta ttctctcttt
     2521 aagcaataga gaattaataa aaaaaacatc cgtgtgaagc cgttaaatcc gtattgctaa
     2581 ttatggtaat tatttgttcc tctacccagt caaccgataa taaactttta gtccccggaa
     2641 atttgatttg acaaaataaa gccgaaacag agtttgactt tttaaggtaa aagaaatgct
     2701 tgctttaagt tccttggctg tagaaagaga aaataagcag gaatttctac ctggcagaga
     2761 aaaataattc ctgttttaaa gaccaaaata taaacacctt tgccaccttt aaatggggaa
     2821 aaggtttttc tttatgccga attcaacata caaaatatga taagaaatag aggggataac
     2881 tgatgcagtc cacaaaagaa gccatccagg gcttactgga aaaagaaaaa aaaatacttc
     2941 aaatgggcgg cgaaaaagcc attgccaaac aaaaacaagg gggcaaatta actgctcgtg
     3001 aacgacttga cctccttttt gatgaaggca cttttcggga actggatatg ttcgtttccc
     3061 accgttgtga taatttcgga atggaaaaaa tagaaattcc ttccgatgga gttattacag
     3121 gccacggttt agtagatgga cgtcccgttt ttgctttcag tcaggatttt acttcccgtg
     3181 gtggttcttt gggagaaatg catgcggcta aaatatgcaa aattatggat atggcattaa
     3241 aaagcggtgt ccctttaatt ggtattcaag attcaggtgg agctcgtatt caggaaggcg
     3301 tggatgcctt aaagggttat ggagatatct ttttccgtaa ttcccgtgcc agtggagtta
     3361 tacctcaaat cacagcgatt atgggtccct gtgcaggtgg agctgtttat tctcctgcaa
     3421 tgaccgattt cgtttttatg gtaaaaaaca ccagctttat gtttataaca ggtcctgatg
     3481 taattcgtgc tgtaacaggt gaagaaacaa ctcaagaaga tttgggtggt gcaatggctc
     3541 ataacagtaa aagcggaaac gctcattttg cgtgtgaaaa cgatgccgat gccattcaga
     3601 aaataaaagt gctgctatcc tatttgcctt ccaataatat ggaagaacca cctcgcataa
     3661 agacaaatga tgatccctgg cgtttatgtc ctgaactgga ttctatcatt ccggatgacc
     3721 ctaaaatgag ttatgatatg cgggatgtta ttcatagcgt agttgacgac ggcatctttt
     3781 ttgagcccca ctattattac gctcaaaatg taattatcgg ttttgcgcgt ttaaatggaa
     3841 gaacaattgg tattgtagcc agtcaaccta caattttagc cggttgtttg gatattgatg
     3901 cttccgataa agcaacgcgt tttgtgcgct tttgtgattc ctttaatatt cccctggtaa
     3961 cttttgtaga tgtgccaggt tatcttcccg gaactcatca ggaatggaat ggaattattc
     4021 gtcacggagc aaaactttta tggtgttatt cggaagccac agttcccaaa ttgacagttg
     4081 ttacccgcaa agattatggc ggaagctata tagctatgag cagcagacat ttaggtgcag
     4141 atatggtttt tgcctggccc acagcggaaa ttgctgttat gggtgctcaa ggtgctgcca
     4201 atattatttt ccggaaagaa attgccgccg ctgaagatca ggaagcaaaa agacaggaaa
     4261 tgattcaaat ttacgaacag gaatttaata atccctatgt agcagctacg cgtggttatg
     4321 tggatgccgt tattgtgcct gcggaaacaa gaaaacgctt aattgacgca ttggaaatac
     4381 tttgcacaaa aagtgaaagt ttgcctccca aaaagcatgg caatatcccg gtttaaacat
     4441 taggaacgca aaaagatgaa agacaataaa gaaatcgctg ccattggagc tgttatagct
     4501 cttattcaaa gtgaagaaac atctataact tataaggaac cggtaagagg aactccttct
     4561 caaccctata ctgcttatgg cagagaactg acaatgcatt accgcgattt aacacaaaga
     4621 agaataatta aaagatgtag ataaaaggag accataaatg gataaacata gttgcgtaga
     4681 aatgaccact atgcattatg aaccagaccg tcccaaagcg gaaaatcctg ttaaagttca
     4741 ggatttaact tttcgggatg gacatcagtc actttttgcc acgcgtggta gaactgaaga
     4801 tatgcttaat gtggcagaac tgatggatca agttggcttt tactctatgg aaatttgggg
     4861 tggagcaact tttgacacta tgcatcgcta cttgggagaa gacccctggg agagaattcg
     4921 ggtgctgaaa aaacatatca ctaaaacacc cctctcaatg ttgttacgag gacaaaatct
     4981 ggttggctat caaaattatg cggatgatgt tgtggaagca tttgtgcaac gctgttgtga
     5041 taacggtatt gatatttttc gggtgtttga tgctctaaac gatttccgca attttataac
     5101 cgccgttaaa ataatcaaga aaaacaacaa gctctttgag ggtgctattt gtttttcttt
     5161 aactgaacaa cgaatgggtg gagatattta taatattgag tattatgttg ctaaagccaa
     5221 acagctggaa gaaatgggtg ccgatacaat ttgcattaaa gatatggctg gcttaattgc
     5281 tccttacgat gcttacgaac ttattaaggc actgaaggaa aatgtgaaag tgccagttca
     5341 tttgcatacc cattttactt ctggaatggg tgatttggct ttatttaagg caattgaagc
     5401 aggcgttgat attatagata cttgtgtagc tccttatgct tatagaactt cccatccggc
     5461 aattgaacca ctggtaattt cccttttggg aaccaatcgc gataccggtt ttgacattaa
     5521 gctcctaaac aaaatcggca aggaaatgga gaaagacatt cccaagtatc tggaatttgc
     5581 tgatactaca aaattctcta taattgatac cgatgtaatt attcatcaga ccccgggcgg
     5641 aatgctttcc aacctggtaa atcaattacg ccaaatggat gctttggata agctggaaga
     5701 tgtgtttaaa gaaatcccca aagtgagaaa agacctgggt caaattcctt tagttactcc
     5761 cacaagtcag attgtaggta ttcaggcagt taataatgcc ttgtttgatt cttatgaagg
     5821 tgaatattcc cggattacag aacaggtgaa agacctttgt tatggtcttt atggaaaaac
     5881 tactcttcct atcaatccgg aagtgcagaa aaaggcatta aaaggttatc ccagaggaga
     5941 aacaccaata acctgtcgcc ccggagatgt tattcctcct gcaatggaac aagtccataa
     6001 agaatgtgag ggcttagcta aaaacattga tgatgaatta atcgttgccc tctataacat
     6061 taccggaaaa aggttcttga gaattaaata tggcttggaa ccaatgccgg aagaaatgaa
     6121 acctaaaacc ctggcagatg ttaaaaaaga acaagaactt attgctaaag ctaaagcagg
     6181 gaaattgata gaaaaaccgg aaccgaaaga taaaccccaa gacctgcgtc agtttgatgt
     6241 ttttgtggat aatacctact atctggtaga ggtttcggaa aaaggaggaa cccctaaaat
     6301 agttaattcg cgtcctgcac ctgttatttc cgaaacaatt gtaaaaccgg ctaataatcc
     6361 caatcctcaa gtaaaacctt ctaatcctgc tccggaacct aaacctgctc ctgtagttaa
     6421 ttccgaaggc ggtacaaaaa ttaccgctcc gatgccggga atgtttatca gatacgaaaa
     6481 aaaacctggt gaccctgtta aacaaggaga aactgtcctt gttctggaag caatgaaaat
     6541 gtataataat attccttcac ctgtagatgg agtgcttgtt gaagcacctt ttaccggagg
     6601 tgctaatgtt gccaaagggg atgtgttagc agttatcaag tgaaaggtga acagtgaaag
     6661 gtgaacagtg aaaggtgaat ggtgaaaagt gaaacaagga gataatttga gttgcaaacc
     6721 tgttttgggt aaccggagga cgcttttcct ccgcattcaa tatgattttt ttaatgaagt
     6781 ttttcttgtc attcctgcga aagcaggaat ccaagttagt tttgaaaata taattacatt
     6841 tccaaagtag aatctgcatt ggagtgaaag gagacaaaag tgaaaccctc aacctctata
     6901 tttcagatga actttccttt actatttaca ccccttgttt cacctttcca ctctttaagg
     6961 aggtaaaatg ctcgttaaaa gccaaataat ggacaaagac caaatggagc gttctctgca
     7021 gaggatggct catgaaatta ttgaacaaaa ccgcggtttg gaaaaaatcc gtctggtggg
     7081 cattcgttcc cggggagttc ctttagcaga acgtctttcc agttatttaa aacttatatc
     7141 caatcaggaa gttccggttg gcattttgga tataacttta tatcgtgatg atctttctac
     7201 cattagtcat cagccggtta taaagggttc cgctattgat tttgatattg aggatgcggt
     7261 tgtagttctt gttgatgatg ttctttatac gggaagaacg gttcgagctg ctattgatgc
     7321 tttaatggat tttggcagac cgaaacaaat tcaactggcg gttttaattg atcggggtca
     7381 tcgggaactg cctattaaag cggattatgt aggcaaaaat gtccccactt ccaaagagga
     7441 aataattaag gtctcactgg aagaaacaga tggcgaagat agcgtcaaaa tcgttttggt
     7501 ataaatatca ggaacgctat caggcaataa aatcggttct ttgtgtaggg ctggattccg
     7561 atttttctca gcttccggaa ggtgttaaaa atagcgagaa tcctatttgg gaatttaata
     7621 aagccattat tgaagcaact ttggattatg cttgtgccta taaacccaat ctggcatttt
     7681 atttggcaga tggaattcgc ggtttggatg ccctttataa gacagtagaa tttattccgg
     7741 ataatcttcc cgtaatttta gattgcaaaa ttggcgatat tggcaatact atgaatgcct
     7801 accttgaaag tttttttaca gacctcaaag ttgatgccat aacccttaat cctcttatgg
     7861 gaagcgatgt tatgcaaccg gttcaggaca gaaatgcttt tgcttttgtg ttgtgtttaa
     7921 cttccaatcc ttctgcctac gattttttgc aagaaggtat ggcagaaaaa atagctaact
     7981 ggttacagga ttatcccaaa gagaatttcg gagctgtagt tggcgcaacg catagcgcaa
     8041 atattaaaac aatgcgtaac ttattaaagg atcgcatatt gcttattccc ggaatcggag
     8101 ctcagggtgg cgacctgaaa accgttttac aagaggcaaa agatagttct gaaacaccca
     8161 atattctggt caatagctca aggggaatta tttttgcctc caaaggaaag gactttgcca
     8221 ctgctgcaag ggaagaagca agaaaattaa cacaagaaat gaagaatgca ttaagcaagg
     8281 ggtagtaaat aaaaaataga actcaaataa ctgtactact tggattagca ggattgatga
     8341 aataagtaat attacatcca agatactttt taatccgtaa aatccgtgtt gttgctctat
     8401 gttatctaag ttgcttattc ttatttttca cttatttttt ttatagagct tacaacgagg
     8461 ttggtttaat attttattta ctctctttaa gcaaagagga ggagaaagat gaagaagatt
     8521 tttattttta cactgctaat ttccgcagta ttctgttttg cctttcaagt tgataccttt
     8581 ggacctactg cagacgattt ttcttgtgcc aatttagacc aggtgccggc aagttttgga
     8641 attcagatag ctaatcagaa aaaactttgc ctctgggaaa atggtatctg gaatagttat
     8701 tcctatttta ctacgggttt aactattaaa ggtacttata tgctaaatgc ggacacgatg
     8761 ctggttgcta tgggaattta ttcttacagt gatggtattt ataactttga tattaacacg
     8821 cataactggc aactgaatga ttggtttata ttgcctaatt ttgtaaagta taatccccat
     8881 aaccaattat attatgtggg agaacgggat ggtttgtttt attcagcgaa tggacataac
     8941 tggaccagga taacatccct gggctcaaat aaatgcaatt ctattgcctg gtatgatagc
     9001 aatatcataa ctaataatgg tcagatggtt tattattcca atgataacgg acttaactgg
     9061 cagcaatcat ccattagcaa tttaagtagt ttcaggtttg acgataatgg cattttatat
     9121 gctattatgg atgtaggaag tgattcagac ggactttggc gttctaatga ttttggcgcg
     9181 aattggacaa atatccttta tacttcagct ctttcttgct taggaccaat ttataataat
     9241 aatgtaaccc ttggttggag agaaagttac gcagatagtt gctttgtcgg tatgctgaat
     9301 tcttcagggg aagtaacctt ttttaatcat cctctactta atgcaccggt taaacagata
     9361 gatatctttc cctatatcaa taccccttct ttttatgttt tgaactctgg gggttgtttc
     9421 tttgtgaccg gttttctggt aggaattgat gaccccgtta ttcctcaaat accacaggca
     9481 aaaatatcta cctatcctaa tccagcacta tcttatgctg atatttattt aagtaaagtt
     9541 agtcctgaac cctgcaaatt aactatctat aacctcaaag gtcagaaagt aaggaatttg
     9601 gaccttgctg aaacattgaa agaacaaacc ttatcctggg atttgaaaaa taataacggt
     9661 tttaaagtat ctgccggaat ttattttctc gtgttacagg atactaaagg aaattgcctg
     9721 gcaaaaacga agttggcggt aattaaatag atacaggctt agaggttgag agggtttaga
     9781 aggttgagag ggtttagatt tcttaaaagc tcggagagcg acacaatgat agcgatggtg
     9841 gcgtaagccc ctcgtaaaat gtaagccctt tttttctttt tccctggttc caaaagctcg
     9901 gagagcgaca caatgatagc tcaaccttct aaactttata aaccttcaaa accttatata
     9961 taaaccctct aaaccttcta aaccttctca accctctcaa ccttatttta accgttgctt
    10021 ttttcggaga taaagatagc tccaagattg tcgggattat atttagcgca gagtttatgg
    10081 tataaggaat caatttttat atacaaaggg ctatcttctt ccaggatttg aattagttct
    10141 tttttgctaa tagctttaag gagtccgtcg gtagttacta ctattttatc tcccggattt
    10201 agagaagaaa ctgctgagct aaaatcaagc tttaggttcg gataatcggt gccaaaataa
    10261 ttggtaatag ggctattagg aatatcttcc cacagcacat tcagtttatt cagccgttgc
    10321 agatattttt ccctgtaaag ccaaatttcg gaatcgcctg catttacggt cagaaatttg
    10381 ccttcctgat agattaaacc gcaaagagtt gtagcggaat caaaaaaatc accttgttgc
    10441 cgattccaca ataccagctg ataatttata tcctgcagga agttatctat tatttcctgc
    10501 cagttttgtt cggcttttaa aggcagatat ttcagctgtt ccaaaacaaa ttgagctgca
    10561 ttttcactgt ttgccaacga gccaattcca tctgctacaa agcaaaagag gttatcaaga
    10621 ttctgcagat gattaattcc ctctgtcaaa aggtctttat ttaccagaac tctgtcctga
    10681 tttttctttc cgctgttgcc gtcaaagcag aaataggaaa catcaaactg cattttccta
    10741 aaatcctttt ttcttttata ttgctatcat agtcgtctca caaatcttga caaggatttt
    10801 tttcaaaact tggaaaaacg aaaaagcatt tgacagataa tcactttagg caaagagggt
    10861 ttagtagatt tagagggatt tttgcgtgcg caggagtaac aaaaaggatt cctgcttaca
    10921 cgagaagaca aatggttttt tatttaagaa atggcataag gagaactaat gcccggaata
    10981 aattgcctga ttaacgctca gggtataact cagcaaaccc aaaataccat agacaattat
    11041 ctgcaaaggt tggaagccac ttttccgcat ctccaattta ccaagcttat gccttgcagg
    11101 gaagtgctga tatcaatttc tcaacatcgg ggctatcggg tttatacaga aaaatacagg
    11161 ggttggcaaa tatttcacga gctacaggat aattcctttt taacccaagc ttttcaagac
    11221 attttagagc aggcggaaat ttctcaaaac ccgttatcca atatgcaaaa taggtggaat
    11281 tccgagctca aagaaattaa caacagtttt ttcctgtttg ccgtaaatcc tgaattacac
    11341 aaaataattc ttgccaatga tgctttagcc cgtttacctg tttactatac aaaacaaaag
    11401 gaatatttcc ttttgggtag ggatatatct tttgttaaag caatcagcaa tcaactaacc
    11461 ttggatcctt tgtttctggc tttgtatctt tccctaactt atgttccggg acgcggaact
    11521 ttttaccggg aaatagacac tttggcgggt ggaactttag gtgtatatga ttggctaact
    11581 gacgaattgc aaataagttc ccagcaggaa ttgaggttta tagagcccct ctttggcagg
    11641 tcgcataaaa aatggctggc agaacttgta gaaacattta cagctatctg tgtttcctat
    11701 catacagagc ttccaaaagt tgtatcttta agcgggggaa tggattcgcg ttgtgtggca
    11761 ggagcgcttc aaagcaaaaa tatagatttt acagcaattt cctttctgga tgccaatcag
    11821 gatgcgcaag atgatgtttt aatcgctgtt cagacagcag aattactgaa aaagacgcac
    11881 agggttttac agctatctcc ttgtgctcct gaacattatg agaagttatt ttccttgaaa
    11941 gcgggtttga actatctttc tgtggctatg tttctgcaat atttggaaat gattttagct
    12001 ctatatcccg aatccgcttt gtttttaacc ggagatggcg gtgacaaagt tatgcgttat
    12061 cttttgcctg ataagcaatt aactgacgaa aaacagtggc tgaattactg gtataatcag
    12121 aatgctgttt tttctcccaa ggcatcagcc gaactatttg gcattcaaca gaaggcgatg
    12181 gaagaatatc tccttaatca tatatctact tatccgacaa aggattataa ctataaatat
    12241 gcttttgctc ttttagcgga acgttctgct cgttgggcat ttgaagggga agaccgcaat
    12301 cgctattatt tccgttccga aacccccttt ttggactatc aattttaccg tctggcaatg
    12361 cagattccaa tggagcaaaa aaaggataac cttttatatt acagcttctt atctgctctt
    12421 tcacctgccc tggcaaaatt gagatacgct catcatcaat ggtcacccgc taaaatgaga
    12481 aatcccttct accgctattt ggttaataaa acacgcaact tccgcattca atatagaaaa
    12541 ccgaaaggaa aaatcaattc tcagcccaaa tttgcagaac aagaatattt aataacccga
    12601 atcctgaaac agagtgaaaa tcccctttta caggcgctta tgccgggtgc aaaaaatatt
    12661 ctcaccttgc cagacctaca aaatttaagc tccaatcaac tgggaactct atacacttgc
    12721 gtatctgtca tcaccggaat agtttagtta tctcttcctt aatagcattg cttacctatt
    12781 gcttacccat tacggatggg attagtaatg ggtgagcaat gcttaagcaa tgcattcggg
    12841 gaaaagaaca tccgtgttct tttctgttta ttccttttat tagcagtgag aactcgtgac
    12901 gacattagcg aaaaggtgga aaagtgaaat ataggaataa aattgaggtt ttaacctgaa
    12961 attataaaat cttatctatg ttacccggga aatctgtgag agaaaaaata aaaaaatccg
    13021 ttaactactt gttgctgata taaaatcgtc acgagtcata ttgcgcctta aaagttatcg
    13081 taaagaacaa agttattaag gcgcaaatga ctcctgactt ttcccgaagg ttgacttcct
    13141 ggtcaaccac ctctcttcct ttatttcgtt gccgaaataa gcgtggtcac aaaaagacaa
    13201 ggttgccaga gaacgctgag gacatcattc ttccgcttac ggatacagca attgacaggg
    13261 ctcagagacc cttatccaat atgctttccc cggttctaaa acacttaaag ttccccctga
    13321 aacaggattc caaacagagg ataagtaacg cacttcctgt gttttagtta aaatagaagc
    13381 tatggcactg gcaacaggta aggaattatc cggcaaataa ggaattaaat tccaacccct
    13441 gtttaaggca ataggatagt ttgaggtatt cagtaactga ccttgaatac tgagattttg
    13501 agcagaagaa ttattcaccc aataaccctt ggcactttgt agcgatgaaa gtgtattaaa
    13561 ataagaaggc atgaagggtg cataactttt tgccgtatcc ttaatctgca gaaggttact
    13621 gccaaaaaca gagctgaaat tattcgttcc caaatctaca ttgaaactga gcaaattcca
    13681 tcccggggta agagtaatct gttgatagtg aggaagttca cccatagtta cggaaatgga
    13741 agtggcagta ttggcattaa caaccactcc actaatggtc ttatcgggaa agccactggc
    13801 agaaatagtc aaattatagg ttccagggct taaataccgg taaaaatcac cctttgcggg
    13861 atcggtaatc actgtggaat agctgttatc atatccgttt acagtaatcg tagcggataa
    13921 aggatttccg taagggtctt gcacaattcc gtgaattcca tacattgcct gttccaaata
    13981 ggaaatcatt gcttcatagt tataattcca atacccaggc aaagttgacg cagaaggcat
    14041 ttttgtgttg gaaatctcta tagtaacttc ccggcagtga gcagaataat tcatccagtc
    14101 ctgtctgcca cccgtaataa cataccagtc agctccattg gttataccgt tggaagaaac
    14161 actggtaaaa taaccggaag gtccattggc aatagcatta ttagcataaa caaaagaagt
    14221 agaaatatac cagttctcat cgggatgagc tatataagta taatcccagg gataatttac
    14281 aacctctgcc ccaccatgaa aattaacact gtaaacaaag tgatgattat ttgccaggtt
    14341 catcatcagg gttgtttcct gctgtaaggg ttgtccacta tactgattgc cgtttggatc
    14401 gggaaaattg cggtttaaat catatccgtt gtagttatat cttctggctc cggaaacaga
    14461 gttattgcct ccataataag ttccatcggg attggtattg ggacaaatcc aaatttccag
    14521 attattaatt aggttggtta aacgctgatc ggttccataa ctgcttaaaa gggtatctat
    14581 taaccgtagc attaaaacaa agcccgtggt ctcatctcca tgaattgttg aggtatacat
    14641 aacttccggt tctgcttccg ctatattaac attatcagaa attttggcaa acagcaaact
    14701 gcgtccgttt accgtagttc cggcatttac aatttggcat acattgggat aattagcggc
    14761 aaaggaattc atcgtgttta cataactatc ataagtagga taactatccc ataactttgt
    14821 ctgtttcaca tcggaactca ttaccggttc aattagagat gctggagagg gcaggagttg
    14881 ggtttttaag cccaactccc tgaatttact gtattcttca tcattggcat aagcataaac
    14941 ccaattaccg cgaacattat caatagaaat tatgttactt atttccggca gagttgtctt
    15001 atctgccaat tcaaaacgga agtaatactg attccactct ttggcaaaaa gggaaaatgc
    15061 caaaagcaga agcagagcaa caaggatggt ttttctcatt tatttgcctt tactgatgcc
    15121 aatttgctcc gctacaacct tgaagaaaca tttttgttgg gtagtgggaa tttcccaata
    15181 taaatttgca gttgtgtgca ccagctgata agttccgtag ggttcactgc aggaataaat
    15241 acgatagtta ttagcattgg gaactgcatt ccaagtaaga cgataggtat tttgatggct
    15301 tacaaggtta acttggggag gtgtaagaac tatattgaaa gtgccattta agacaacagg
    15361 attttcagca gaaccatata cacttcctgt ttgcatagta agggtctcct gcacaggata
    15421 aacagcatca ttcgcataag aataaatctt gaattgaacc agttcaccat cggaagcaag
    15481 atttactaag atggtagaat aggcagtagc tctatcaatt aacacaattt ctcctgtgcc
    15541 tctacattca ttattcacaa aagcacccac caaatcattc agctgagccg gaatgccttc
    15601 aatagacact tgggcatata ccgtagcagg attattagga taaacaatag gttgccaatc
    15661 aggaacatta acaggattaa ctattactat tacattatcg taagcagtta gttcaccatc
    15721 gctaacacca aaagtaatat tttcactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnagg atgaacggga tcgttgcttt gaatagttaa aatcgcatta taaatacctt
    15901 ctgccattcc caccgctgaa aaatgaccgc taatagtttg atttccacct gctgtaatag
    15961 ttccgctatt gggagttaca ctaaaccatt ccaccggagg aagagaacag gcaagcgaaa
    16021 tttctccgct tacagtattc ggttctgcac cccaaatatc accatttatt gtatagggaa
    16081 gaacaattgt agtaaccaat ccggcactga tagttacatt tactgtagcg gtagcagatt
    16141 ctgaaccgtg aataactccc catccatttg aaccggttcc aaaccaacta atggtaatac
    16201 ctgttccggt tgttagatca ggaattaagt cacctccact accaccgaca aaattagtag
    16261 cactatttac agttactccc gcagggaaag taactataac ttctttcagc cattcagtat
    16321 cagtgctagc attagttaca gtgaaagtcc agtcaaccgt tgtgccggaa gtatactcat
    16381 ctgcattcaa agttaaagta gagccggcaa tacttttact gccggttgtt ttggctggaa
    16441 taatgacttc atttctgcca gtaggttcac taactaccat agtataagtt aaggattgtg
    16501 agccggtatt actgattgtg aaagtttgag tgctttccgc tccggtagtc aaagtagcag
    16561 ataaagtatt ggtatttaca gatatagtgg gaggaagata tccgctgcct gtaaccggaa
    16621 gagataaatt agcttgggtc tgactgttgc tgctaatagt aacattgcca ttatagctga
    16681 ttgcagagac aggtgaaaag cttaaaatat aggtcttgct ctgtcctgca ggaatggaaa
    16741 agctaagagt attgcgaatt tcttttgctg cagcggtaac ggaataaccg gtgggagtaa
    16801 ttatagaacc ggtaagagta gaaccgccgg tattagtaat cgtgaagttc tgcgtggaag
    16861 ttgttcccac cgctaccgaa ccaaaggtaa aagaagtagg atttacagat atttgtgggt
    16921 taccagcaac ggcagttaag aacaatttta cattaggttt ataaacagag gttgaaccac
    16981 tgctaaacac attggtttga tcagcagaat cattacgcgt aaatctataa ccatttgcca
    17041 tagaagtata ttgcactgtc cctgttgagc tccaacttcc tatcaaacca aaggcagtat
    17101 caattaaaag gttatctgtt ccgttccaca gaaaaggagt gcttaaagta agcatattgt
    17161 aaccggttgt tgaaatagta tagctggcgt tggtataaac cgtaaccaaa ttcgtattat
    17221 caatccaact gctaacatct gtggctgtgg tatgtttcat tctcactaca aaattgggca
    17281 ttgttttatt gggcacgcca gttacattaa atcctacctg tgagatataa acaggaccaa
    17341 ataccccggc attatttaat tcggctttcg tataaaccga ttgaccatgc aagctttgat
    17401 aataaacatt gattgg
//
LOCUS       DAOOZV010000038        17074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3416_length_17074_cov_67.199542, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000038 DAOOZV010000000
VERSION     DAOOZV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17074
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_17074_cov_67.199542"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..3007)
                     /locus_tag="PLW08_05105"
     CDS             complement(8..3007)
                     /locus_tag="PLW08_05105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPL52017.1"
                     /translation="MKHFTVFLALLVAFGLFAADLKENEGTAIFKLKPEFRSALQKSD
                     NRTGIASIDEKLQKLQVSSLSPKFSISPQKREECELSLIFTVQSPYPPQAVVNLLSSD
                     PCVQYAEIVYPDVIFAVPNDEHYSESRYFASLEAESAWDIHKGENGTQPVIIALVDTG
                     CRWTHPDLAENIWQNLGEDINHNGYTIYYNGSTWVMDTGDLNGIDDDGNGKIDDLIGW
                     DFMATSNGGETNNPYENSGHGTNVSGILSARTNNTIGVSSLAWNLTLMPVSCSYPGSS
                     SIYNGYQGIIYAAENGADIINCSWGGTTYSQANQEAINYAYSLGSIIVAAAGNSNNTV
                     PVYPAAYQNVLAVAALQNNGVKSSVSCYGAFVDVGAPTGSIGTLSGSGYTTVSNATSY
                     ASPVGSSLAGLIKSYYPEISQSTLLNRIKGSCDDVDAVNLGKENLLGEGKLNARRALQ
                     EDNPRSDEEIRLALIENRGATDANGNLAVEPGETFSVNLLLRSYGEYSANGTFTLSTT
                     NPNVNILSGSYNQSIPADAYFTLEEVFNIYVLPTATSQYVTFNLQTTADLPVVAGNNL
                     SFSILINAGGIFVWEGISGGRDMSGTFIKNTLQNLGYNCVYGTTFPASFYSFDAVFLS
                     FGAVGSNIVRFDKPYMFNALYNYLISGGRVYIEGVDVVGFDLTYYLPDIQGELDAYEV
                     LWPLLGIIGAEDGVTNSIDNLSGVSYTPSSGMLFTSSSQTKVDYIDRFTELYPYARSA
                     FEESDYGCVAVVCAGGYNQRSFVFSYALSELTDGTFPNTRANLVTRIMDFFTAEEVTL
                     PVELTSLYATYSNGATVFWNTASENNVLGYNLYRNSNFSLSNAVKLNTVIIPATGCAT
                     GNNYCYYDSETALSDTLYYWLESVSYSGFTQIFGPAVLIVPFTEPETPPLPPDKIEYL
                     SAYPNPFGNSVFLELKIASPAPVMVKVYNIKGQLIKNWEYPYLETGIHHLSWDGLDNK
                     QRNTTSGIYLMVVKTQERTFTRKLLKM"
     gene            complement(3017..3370)
                     /locus_tag="PLW08_05110"
     CDS             complement(3017..3370)
                     /locus_tag="PLW08_05110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPL52018.1"
                     /translation="MACEFCNLDCSVYLAENEHFFAIWDKYPVSKGHTLIISKRHTPD
                     YFNLNAEETISLHDITLKVKKVLEEKFEPRGYNLAMNCGSVAGQSIPHFHLHIIPRFG
                     KGDKGLFPRRRESVF"
     gene            3990..5450
                     /locus_tag="PLW08_05115"
     CDS             3990..5450
                     /locus_tag="PLW08_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL52019.1"
                     /translation="MKYKVQIVLAICLLWTMSLISAKPDPNALVGKIGDREYNYKKFN
                     DGFKSYLQYHGKGKVLTKQDSIKLNNQYWEELIGMYIYDQVIKAGKIKVTDAELEAEI
                     KKNIPAEIKQIKDFQTNGKFDKAKYEQALKDHPDFKREVMNYSRDLYSYNKLIKTIKN
                     EVKANPDSVKNAWLKENDYASAKIIHFDYNKLTSVNATDDEVRAFYEAHKDEFKRENG
                     RSYLLARFPGNLSKAENAEQKAQENKILSTELYNRAKEVGLQKAAEEMNIPLEESPYF
                     NSQDQIIPHIGRAPDLVSFAFNNPVGSIPDIFYAPTGDILVLELKSETPEYYIDFEIK
                     KQEMIIRANRTKRMYTMENYVQNFMQSETPETYLEAAIRDSIAIIEAEKVIADNEIKP
                     LGKIPALNTAILNTPEGSFTPLIENEKHWYLALVTKRQYPDLTLWEKDKKKIIADAEA
                     KLQEDHLNQWYFEQRQKVQIIDNRKDFYELQMQLKL"
     gene            5673..6227
                     /locus_tag="PLW08_05120"
     CDS             5673..6227
                     /locus_tag="PLW08_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52020.1"
                     /translation="MKRLFILLLLLTGCFGLNAQTGLFNLSYAIPIEEADSLLALSDF
                     YTPNINDNLVRYYPENSNYVEAILVFVEPQTRRIIGWFIKYSPDNSEEDDAYVLQTLQ
                     EMHGKNNYFDEETQQLIWFLSTTRSVHIMYADDNSLTVLYYDSYFPELFHLKDKKALR
                     KDSETENNPLPEDSKPAPSAEDTR"
     gene            6342..6632
                     /locus_tag="PLW08_05125"
     CDS             6342..6632
                     /locus_tag="PLW08_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52021.1"
                     /translation="MNIHFRDVQSGSVEARAIIEIADGVFLNEVTILNIDGEIVVEFP
                     RKSFVGKSKRTYYIDIITFEDNDKRIVWELEIKNAYREWRKANKKVLIYEEK"
     gene            6656..7870
                     /locus_tag="PLW08_05130"
     CDS             6656..7870
                     /locus_tag="PLW08_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPL52022.1"
                     /translation="MITDLQNILSTNIKGMKRSAIRELLKFLGQPGLISFSGGFPSPL
                     SFPIEELKSIIAEVMENEAAFALQYGTTEGDDLLRTMLANRYKANGIDVTKDNIIITT
                     ASQQALDLISKMFIDPGDDVIVGLPSYLGALQAFGSYGANMIGIPMDDEGEDPNLMEE
                     ALKDLASKGKKPKFIYLIPDFQNPAGITMSERRRKEMLELAHKYDVLILEDSPYRELR
                     YEGETQRTLYELDGTGQVVILGTFSKIFCPGFRIGWVVGNPDVLDKIVVGKQATDLCT
                     PPFTQRIAARYMEKGYLDPKIEDIKKMYSVKQKVMLESLEKYMPEEMKWTHPQGGLFL
                     MVTGPEDLDTNALLLECIKEANVAYVAGNSFFCNGKGYNTMRLNFSYESIETNIEGCK
                     RLGSFFKKVLQK"
     gene            7890..8189
                     /locus_tag="PLW08_05135"
     CDS             7890..8189
                     /locus_tag="PLW08_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL52023.1"
                     /translation="MSKKVEKDRMGMIIHEEDTPEKMEVELTEIKAPGDDDSPVLIYY
                     NWCKKCGICVAFCPTGCLGRKSDGSPFVQAPEKCTHCENCDRLCPDFAITGAKER"
     gene            8201..9445
                     /locus_tag="PLW08_05140"
     CDS             8201..9445
                     /locus_tag="PLW08_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPL52024.1"
                     /translation="MTEKDKSKKTKHKTTSDKASLAAKPESKLEEIQAKRERKTVLMQ
                     GNEAVAYGALDAGVNFFAGYPITPSTEIAEILAAELPKRNGVFIQMEDELASICAIIG
                     ASLAGAKSLTATSGPGFSLMQEGIGFAKITETPCVVVDVQRVGPSTGMPTSPAQGDIM
                     QSKWGSHGDSPAIVLYPDSVKESYELTIRAVNLSEKYRTCVILLLDEVIAHMREAVCL
                     PDLANVRIINRIKPTVPPHWYKHYDENQKYLSPLASFGEGYRFHVTGLTHDSHGFPTN
                     KPSEAAEMMERLRKKISYNIRDLVQIESYQMEDAKIAIFAAGITSRAAKAAIASARAE
                     GVKVGLLRPLTIWPFPDDAVRKMLRNVETVIVPELNQGQLIHEVQRLTKEKSDGSLIP
                     INKVSSELITPNDILNKIKEVC"
     gene            9447..10286
                     /locus_tag="PLW08_05145"
     CDS             9447..10286
                     /locus_tag="PLW08_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPL52025.1"
                     /translation="MANIPQRIIHEYLRHDKKFPHVWCAGCSNGIVLGAIIRAVASLN
                     IDRNDIVMVSGIGCSSRMPVYVDFNTLHTLHGRSIAFATGVKMHKPHLKVIVVTGDGD
                     CAAIGGNHLIHAARRNIDLKVIMINNNIYGMTGGQCSPTTPHNAYATTAPYGNIEPDF
                     NICNLAMGAGASFVARTTAFHVLEMQSMIKDALQHPGFSLIEVVSACPVIYGRLNKKG
                     GAAQMLKDFRDNSLPLAAVEKLPPEKVEGKIIRGILRKEIRPEFAAEYAALCKRVQTE
                     GGE"
     gene            10286..10822
                     /locus_tag="PLW08_05150"
     CDS             10286..10822
                     /locus_tag="PLW08_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPL52026.1"
                     /translation="MAKTFEVRLSGSGGQGLILAGIILAKAAVLENRKVTQTQSYGPE
                     SRGGYSHADVIISDKEIYFPEATNINCLLALTQEACDKFLFDLCENGILIIDTTFVKN
                     LALAAENTYEIPFTEIAQEKLGSPISTNIMALAFLVKITGIVKESSLKKAIEETVKPA
                     FVDLNLKAMQIGFDLAKK"
     gene            11057..11458
                     /gene="mce"
                     /locus_tag="PLW08_05155"
     CDS             11057..11458
                     /gene="mce"
                     /locus_tag="PLW08_05155"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPL52027.1"
                     /translation="MLKHISHIGIAVKDLEAGIAFYEKLGLKLEGIEEVPSQKVKVAF
                     FPCGETRIELLCPTSEESPIAKFIEKKGEGIQHIAFAVDDIEASLKQANELGLALIDK
                     EPRPGAHHSDIAFLHPKSALGVLIEFCQEHG"
     gene            11745..12059
                     /gene="rplU"
                     /locus_tag="PLW08_05160"
     CDS             11745..12059
                     /gene="rplU"
                     /locus_tag="PLW08_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPL52028.1"
                     /translation="MYAIVEIKGFQYRAEKNAVLRVPYLKTAEPGQTLTFDKVLLLRE
                     EEEIKVGQPVVEGASIVAEVIENGKGEKKIIYHHKRRKGYRVKKGYRESYTDLRVTDI
                     LA"
     gene            12076..12339
                     /gene="rpmA"
                     /locus_tag="PLW08_05165"
     CDS             12076..12339
                     /gene="rpmA"
                     /locus_tag="PLW08_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPL52029.1"
                     /translation="MAHKKGVGSSRNGRNSNPKYRGVKKHASESVLAGNIIVRQKGTK
                     IHPGKNVGIGRDYTIYSLIDGKVTFTIGKEGRKYVSVQPMNEE"
     gene            12491..12916
                     /locus_tag="PLW08_05170"
     CDS             12491..12916
                     /locus_tag="PLW08_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52030.1"
                     /translation="MEAQQILLLILSIIIIGTTIIVGITLYKDQAYTANKTALVAEAK
                     NFGKRITKYCQDLASLKNDNLQSASVDTTKLIKYLGWEGNFIKTEAGTFNITAVSDSS
                     VIITGYAKAKKNGKSPKVIVTVTFPEGKMDLVESDSVTK"
     gene            13002..13379
                     /gene="gcvH"
                     /locus_tag="PLW08_05175"
     CDS             13002..13379
                     /gene="gcvH"
                     /locus_tag="PLW08_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPL52031.1"
                     /translation="MEIRDDLYYTDTHEWIHLDGDIATIGISDFAQHELGDIVFVELP
                     EVGQKVAASEPCGSIEAVKAVEDLISPLSGKVEEKNIDLEDSPELINKSPYEEGWLFK
                     IRMSNPEELENLLSAAEYQKIIP"
     gene            13405..14034
                     /gene="gmk"
                     /locus_tag="PLW08_05180"
     CDS             13405..14034
                     /gene="gmk"
                     /locus_tag="PLW08_05180"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPL52032.1"
                     /translation="MITKDNHFLIILSAPSGGGKSTILNEILKVTNDIDYSVSYTTRS
                     PRGTEQNGIHYHFVTEEEFQRRIDEGDFLEYAKVFGNWYGTSKSFIKDRLAKKHHVIL
                     DIDVQGASLISATDIPYVKIFILPPSMEVLHQRLVLRATDSEEEIAKRLQIAKDEVAY
                     IPQYDYLVINDKLDIAVQDVLAIIRAEENRVCRYNHPAMDFISTGEKDD"
     gene            14027..14416
                     /locus_tag="PLW08_05185"
     CDS             14027..14416
                     /locus_tag="PLW08_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52033.1"
                     /translation="MINEKLESFLEKADMNYLFCLLAQLEAYRLLQLPTYVKQKFQDK
                     ITVMAMNHVAENEVPDYITELAEAELAEAQESPFQEEEGAEEEAEETIDDSKKFIEEL
                     TGTEDYEEEEEEEEEFYNEDNEDNDES"
     gene            14420..15073
                     /locus_tag="PLW08_05190"
     CDS             14420..15073
                     /locus_tag="PLW08_05190"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPL52034.1"
                     /translation="MSVNALRQYLEFLKNSGIKELYYPPSDKQKKLTELQKQYSTCTK
                     CPLHNSRHKFVYGEGNPNAIAMLIGEGPGEQEDITGRPFVGAAGQLLEKMLSAINLKR
                     SDVYITNIVKCRPPGNRNPESEERKACLPYLLEQIQIIQPKLLLILGLVAAQTLLNNN
                     NTLGWHRGKIHYVMDIPAFVTYHPSALLRNQEWKKPAWIDLQEFQKEYEKLLAATIS"
     gene            15217..16044
                     /locus_tag="PLW08_05195"
     CDS             15217..16044
                     /locus_tag="PLW08_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPL52035.1"
                     /translation="MIKQRNSPLSIFKLNLPISLLITFLLTFLVVGLDARFAIYGDTR
                     SNPKIHQQIVQQIVSRKPEAVFHTGDLNSSGKTQKEYDNFLQIISPISQSARFFPARG
                     NHEKDLTLFLQNFPYLNGSSYYTVFQDGIRFIILDSVLDLSPGSEQFSWLQKQLKEDI
                     PSLLILHHPVFSSGEHNDELGLQLFLPELLQNTSVKAVFSAHDHNYERSEFKGITYIV
                     TGGGGAPLREKVHENPYSLVFVKTHHYLIGEREDKSIIFKVFNLDNELIDSFTLNGM"
     gene            16046..16897
                     /locus_tag="PLW08_05200"
     CDS             16046..16897
                     /locus_tag="PLW08_05200"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HPL52036.1"
                     /translation="MPHPKVLAIHDLSGFGNTSLMAIIPIMNRMGIEVTAMPSALLSA
                     NTDYPDYILQDNSELLKKSLTHWKELQLSFDAVYTGFLGSPAQVYLLLENLPSLLKKE
                     GIVLVDPVLADEGKLYSCYTWEMVEAMRSLVSISHIITPNWTEAIFLSGYKNRRDLKT
                     IQVYECCKKLAALGPQNIVITSAPDSQGNFSSVAFFAAEKNFFREFECNYAPVIFPGA
                     GDCFSALLLAGILNGCDIVQSIEGTIAFLQEAITISIPVVQDRKMGIDLISALKKDPH
                     LFYSQKE"
BASE COUNT         5593 a         3194 c         3379 g         4908 t
ORIGIN      
        1 tcaccactca catttttaac aatttacggg taaatgttcg ttcctgtgtt ttaacaacca
       61 tcaaatagat tccggaagta gtgttgcgct gtttattatc caagccatcc caggaaaggt
      121 gatgaattcc tgtttccaga taaggatatt cccagttctt aatcaattgt cccttaatat
      181 tatagacctt caccataaca ggagcgggag aagcaatttt caattccaaa aaaacagaat
      241 tgccaaaagg attgggataa gcgcttaaat attcaatttt atcaggtggc agaggaggtg
      301 tttcgggttc agtaaaaggc actatcagaa cagcaggacc aaaaatttga gtaaaaccgc
      361 tgtaagaaac cgattccaac caataataca aagtatcgga caaagcagtt tcagaatcgt
      421 aatagcaata attatttccc gttgcacaac ctgttgccgg gataattaca gtattcagtt
      481 taaccgcatt ggacaaagaa aaattgctat tccggtaaag attgtagccc agcacattgt
      541 tttcggaagc agtattccag aaaacagttg ccccattaga gtaggtagca tacaaagatg
      601 taagctcaac cggcagagta acttcctctg ctgtgaaaaa atccattatg cgagttacaa
      661 ggtttgctct ggtatttgga aaagtgccat ccgtcaattc gctcaaagca tagctgaaga
      721 caaaactccg ttgattatag ccaccagcac aaacaactgc tacacatccg taatcacttt
      781 cctcaaaggc acttctggca taaggataga gctctgtaaa gcggtctata tagtccactt
      841 tggtttgcga agaagaggta aatagcattc cggaggaggg tgtataagaa acaccgctaa
      901 ggttatctat agagttagtt acgccatctt cggcaccgat tattcccaaa aggggccaga
      961 gaacttcata agcatccaat tcaccttgaa tatctggaag ataataagtt aaatcaaaac
     1021 cgacaacatc cactccttca atatagaccc tgccaccaga aattaaataa ttataaaggg
     1081 cattaaacat atagggttta tcaaaacgca cgatattact gcctaccgcg ccaaaactta
     1141 aaaaaaccgc atcaaaacta taaaatgaag caggaaaagt agttccgtaa acacaattgt
     1201 agcccaaatt ctgcaaggtg tttttaataa aggttccgct catatctctt cccccggaaa
     1261 tgccttccca aacaaatatc cctcctgcat taataagaat gctgaaagat aggttatttc
     1321 ctgctacaac cggtaaatcc gcagttgtct gcaaattgaa ggttacatat tgagaagttg
     1381 cagttggcag aacataaata ttaaagacct cttccaaagt aaaataagca tcggcaggaa
     1441 tgctttgatt gtagctaccg gaaaggatat ttacattagg attggtggta gaaagagtaa
     1501 aagtgccatt agcagagtat tcaccgtaac ttctcagcaa cagatttacc gaaaatgttt
     1561 cccctggctc cactgccaaa tttccattag cgtctgtagc acctctattt tctattaagg
     1621 caaggcggat ttcctcatca gaacgaggat tatcttcttg cagagcacgc cttgcattca
     1681 atttgccttc acccaaaaga ttttcttttc cgagattaac tgcatcaaca tcatcgcaac
     1741 ttcctttaat tctattgaga agagtacttt ggctgatttc gggataatat gatttgatta
     1801 agcccgctaa agagctccct acgggactgg cataagaagt agcattacta actgttgtat
     1861 aaccactacc ggaaagagtg ccaatggaac ccgtaggagc accgacatcc acaaatgcac
     1921 cgtaacaaga aacggaagat ttcactccgt tattttgcag agcagctact gccaaaacat
     1981 tttgataggc ggctggatag acaggaactg tgttattact attacctgca gctgcaacaa
     2041 taatagaccc taaagaataa gcataattta tagcttcctg atttgcctgc gaataagtag
     2101 ttccacccca actgcaattt atgatgtccg ctccgttttc agccgcataa ataataccct
     2161 gatagccatt atatattgaa cttgagccag gataactgca ggaaacgggc attaaagtta
     2221 aattccaggc aagggaactg accccgattg tattatttgt tctggcagaa agaattccgg
     2281 aaacatttgt gccatgacca ctgttttcat aaggattatt ggtttcccct ccattacttg
     2341 ttgccataaa atcccaacca atgaggtcat caatttttcc atttccatca tcatcaattc
     2401 cattcaagtc acccgtatcc ataacccaag tgcttccgtt ataataaatt gtgtaaccat
     2461 tgtggttgat atcttcccct aaattttgcc aaatattttc tgccaaatca ggatgagtcc
     2521 atctgcagcc ggtatctacc agagcaataa ttacaggttg agtgccattt tcacctttat
     2581 gaatatccca ggcggattct gcctccagag aagcaaaata gcgagattct gaataatgct
     2641 cgtcattggg aacagcaaaa atgacatcgg gataaacaat ttccgcatat tgcacacagg
     2701 ggtcagaaga aagtaaattt acaactgcct gaggcggata aggactttgc actgtaaaaa
     2761 ttagagaaag ttcacattcc tctctttttt gcggcgaaat gctgaattta gggcttaagg
     2821 aactgacctg caatttctgc agtttttcat ctatagacgc aattcctgtg cgattatcgg
     2881 acttttgcaa agcagaacga aattccggtt tcagcttaaa aatagctgtt ccttcatttt
     2941 cctttaaatc agcggcaaaa agtccaaaag caaccaataa agctaaaaaa acggtaaaat
     3001 gtttcatttc cgcctcctaa aaaacggatt ctcgccttct tggaaagaga cctttatctc
     3061 ctttaccgaa acgaggaatg atatgcagat gaaaatgggg aatactttgt cccgcaacag
     3121 aaccgcaatt catagccaaa ttgtaaccac gcggctcaaa tttttcctcc aaaacttttt
     3181 tcaccttcag agttatatcg tgcaaagaaa ttgtttcttc cgcatttaga ttaaaataat
     3241 caggtgtatg acgcttggaa ataatcaggg tatgaccctt gctaacagga tatttatccc
     3301 aaattgcaaa gaaatgttca ttttctgcca gataaactga gcaatcaaga ttacaaaatt
     3361 cacaagccat aagttgttac ctctttttta tataataatg cattctttca gctaagccaa
     3421 aataatgttt ggcagggaat aaaaacaagc tctttaggta taatgtttca atatcgctta
     3481 ttctttgcta aaatctgctg taatatagat gtaaataaat caggtaagaa atctctttat
     3541 ccttttaact cactataata ttatatagtt agctgtatat tttgcctttc ttgttttaag
     3601 atgtgtattt gcttggtata tagacgatta agtaacgact ccgtaacact tccgtaacac
     3661 ttccgtagtt tcgttacgga gttgttactt gagtgttact tgtttattac aagatcaacc
     3721 tggaaggtta acctcccggt caaacacaat aaaaatcagg agccatttgc gccttaacat
     3781 ttttattatt tccgatatct ttacaagcgc aatataactt gcgacagatt gtgaagcagg
     3841 agttgcagtg agctccggct acacaaatta aaaatggcaa gatatcgttt tcaaggattt
     3901 tggtgcaatt tttttcttgc agtaaaagca ttgtttcaaa agtattgttt tatagaggca
     3961 attatgtctc ttattttgag gtgtaagaaa tgaaatataa agtgcaaatc gttttggcaa
     4021 tatgtttatt gtggacaatg tccctaatct ctgccaagcc cgatcccaat gctttagtcg
     4081 gtaaaattgg agatcgggaa tacaattaca aaaaatttaa tgatggattt aaatcatatc
     4141 tccaatatca cggaaaagga aaagtcctca ccaagcagga tagcattaaa ttgaataatc
     4201 aatactggga agagcttatt gggatgtata tttatgacca ggtaataaaa gctggtaaaa
     4261 taaaagttac cgatgccgaa ctggaagcag aaatcaagaa aaatattccc gcagaaatta
     4321 aacagattaa ggattttcaa accaatggca aatttgataa agccaaatat gaacaggcat
     4381 taaaagacca tcccgatttt aaacgggaag ttatgaatta ttcccgcgac ctctattcct
     4441 ataacaagtt gattaaaaca attaaaaatg aggtaaaagc taatccggat agcgttaaaa
     4501 atgcttggct gaaagaaaat gattatgcca gtgccaaaat tattcatttt gattacaata
     4561 aattgacttc tgttaatgct actgatgatg aagtccgagc attctatgaa gcccataaag
     4621 atgaatttaa aagagaaaat ggacgcagct atcttctggc tcgttttcca ggcaatttga
     4681 gtaaagcaga aaatgcggaa caaaaggcac aggaaaataa aattttaagc acagaacttt
     4741 ataaccgggc aaaagaagta ggtttacaaa aggcagcgga agaaatgaac attcctctgg
     4801 aagaaagtcc ctattttaat tcccaggatc aaattattcc tcatataggt cgtgctcccg
     4861 atttggtttc ttttgccttc aataatcccg ttggttccat tccggatatt ttttatgctc
     4921 ccacaggtga tattctggtt ttggaattga aaagcgaaac ccctgaatat tacattgatt
     4981 ttgagattaa gaaacaggaa atgattatcc gggcaaatcg caccaaacga atgtacacaa
     5041 tggaaaatta tgtgcagaat tttatgcaaa gtgaaacacc ggaaacatat ttggaagcag
     5101 caattagaga tagtattgct attattgaag ctgaaaaagt gattgccgat aacgaaatta
     5161 aacccttagg caaaattccc gctttgaata ctgcaatttt aaatactccc gagggctctt
     5221 tcacacctct tatagaaaat gaaaaacact ggtatcttgc cctcgttacc aaacgccaat
     5281 atcccgactt aactctctgg gaaaaagata aaaagaaaat cattgccgat gcagaagcaa
     5341 aattacagga agatcatctc aatcaatggt attttgaaca acgccagaaa gttcagataa
     5401 ttgataaccg gaaagatttt tatgaattgc aaatgcagct gaaattgtag gaggtcttga
     5461 ggttttgagg ccttgaggtt taacacactc taaacagaga aaatggtaat ataattgtaa
     5521 atatttcatc ctgaaaatcc ccaaaatcct gttcatatca gacctattct ttcatctgca
     5581 caatcggtga actgcttcat tttttcagat tttaattgac gaaccttcta tgcctttaaa
     5641 aatggaaaaa atattatata atggactacc taatgaagag attatttatc ctcttacttc
     5701 tgctgacagg ttgttttgga cttaatgccc aaaccggact ttttaattta agctatgcca
     5761 ttccaattga agaagccgat tccctgcttg ctttaagcga cttttacacc ccaaacataa
     5821 acgacaactt agtgcgctat tatcctgaaa atagtaatta tgtagaggca attctggttt
     5881 ttgtagagcc ccaaacaagg cgtattattg gctggttcat aaaatacagt cctgataatt
     5941 ctgaagaaga tgatgcttat gtgcttcaaa ccctacagga aatgcatggc aaaaacaatt
     6001 attttgatga agaaacacaa caacttatct ggtttttgtc cactactcgc agtgttcata
     6061 ttatgtatgc tgatgataac agtttaacag ttctctatta tgattcttac ttccctgaac
     6121 ttttccacct caaagataaa aaagctttac ggaaagattc tgaaaccgaa aataatcctt
     6181 tacctgagga ctctaaacct gcaccttctg ctgaagacac gagataatta gttttatcta
     6241 aaaaatattc atacttttaa aagtatacta tgtagcaata aataacttga ccaattaaag
     6301 catttatttt ttaaagaaaa agataagaat tgggagtgat tatgaacata catttccgag
     6361 atgtccaaag tggtagtgtt gaagccagag caattataga aattgcggat ggcgtttttt
     6421 taaatgaagt taccattttg aatattgatg gtgaaatagt agtggaattt cctcgcaaga
     6481 gttttgtagg taaatccaaa cgcacctact atatagatat tattactttt gaagataatg
     6541 ataaacggat tgtgtgggaa ctggaaatta aaaatgctta ccgggaatgg cgaaaagcca
     6601 acaagaaagt gctaatttac gaagaaaaat aaaaaaagaa aaggaggatc ataaaatgat
     6661 caccgatttg caaaacattc tctccaccaa cattaaaggt atgaaacgtt ctgctatccg
     6721 tgaactttta aagtttctcg gacaacccgg tttgatttca ttttccggtg gttttcccag
     6781 ccctctttct ttcccgattg aagagctgaa aagcatcatt gccgaagtta tggaaaacga
     6841 agctgctttt gcacttcaat atggaacaac tgaaggtgat gatttattaa gaactatgct
     6901 ggctaatcgt tataaagcca atggcataga tgttaccaaa gataatatca ttattacaac
     6961 tgcctctcaa caagcattag accttatttc caaaatgttt attgacccag gtgatgatgt
     7021 tatcgttggc ttgccttcct acttaggagc acttcaggca tttggttctt atggagctaa
     7081 tatgattggt attcctatgg atgatgaagg tgaagaccca aacttgatgg aagaagcatt
     7141 aaaagacctt gcttctaaag gtaaaaaacc taaatttatc tatctgattc ccgatttcca
     7201 aaatcctgct ggaattacaa tgagtgaacg ccgccggaaa gaaatgctgg aactggctca
     7261 taaatatgat gttctgatcc tggaagatag tccttaccgt gaacttcgct atgaaggtga
     7321 aactcaaaga actctctatg aacttgatgg aacaggtcag gttgttattt taggcacttt
     7381 cagcaaaatc ttctgccctg gttttagaat tggctgggtt gtaggcaatc ccgatgtttt
     7441 agataaaata gttgtaggaa aacaagcaac cgatttatgt actccgcctt tcactcaaag
     7501 aatagctgcc cgctatatgg aaaagggtta tcttgatccc aaaattgaag atattaaaaa
     7561 gatgtattcc gtgaaacaaa aagtgatgct ggaatccctg gaaaaatata tgcccgaaga
     7621 aatgaaatgg actcatcctc aaggtggttt gttcttgatg gttacaggac ctgaagattt
     7681 ggatactaat gctcttctgc tggaatgtat taaagaagca aatgtagctt atgttgctgg
     7741 aaatagtttc ttctgcaatg gcaagggcta taacacaatg cgtttgaatt tcagctacga
     7801 aagcattgaa accaatattg agggatgcaa acgcttaggt agtttcttta aaaaggtatt
     7861 gcaaaaataa ataagtattg gaggaaagaa tgtccaaaaa agtcgaaaaa gaccgtatgg
     7921 gcatgattat acacgaagaa gatactcccg aaaaaatgga agtagaacta acagaaataa
     7981 aagctcccgg cgatgatgat tcacctgtcc tcatctatta taactggtgc aaaaaatgcg
     8041 gaatctgtgt ggctttttgt ccaaccggtt gcctaggaag aaaaagtgac ggttctcctt
     8101 ttgtgcaggc accggaaaaa tgtactcatt gcgagaattg tgatcggctt tgtcctgatt
     8161 ttgccataac cggtgccaag gaacgctaaa ggagtttata atgacagaaa aagataaaag
     8221 taaaaagacc aaacataaaa ccacctccga taaagcatca ctggctgcaa aaccggaaag
     8281 caaactggaa gaaattcagg caaaacgcga acgcaaaaca gtgttaatgc aaggcaatga
     8341 agctgttgct tacggtgctt tggatgctgg agtgaatttt tttgccggct atcctattac
     8401 tccttccacg gaaatagcgg aaattttagc cgctgaactt cccaaaagga acggtgtatt
     8461 tattcaaatg gaagatgagc ttgcctctat ttgtgctata ataggtgctt ctttggcagg
     8521 agctaaatcc ctcactgcta caagcggacc gggtttttct ttgatgcagg aaggaatcgg
     8581 ttttgccaaa attacggaaa caccttgcgt agttgttgat gttcaaagag taggacccag
     8641 cactggaatg ccaacaagtc ctgctcaagg tgatattatg caatccaaat ggggttcgca
     8701 tggtgattct cccgctatag ttctttaccc ggattccgtt aaagaaagct atgaactaac
     8761 tatccgggct gtgaatttaa gcgaaaaata ccgcacttgc gtaattttgc tgttggatga
     8821 agtaattgct catatgcggg aagcagtttg ccttcctgat cttgccaatg tgagaattat
     8881 caatagaatt aaaccgactg tgcctcctca ttggtataag cactatgatg aaaatcaaaa
     8941 atacctttct ccccttgcca gttttggaga gggctaccgt tttcatgtta ccggtttaac
     9001 tcacgattct catggctttc cgacaaacaa accaagtgaa gcagcagaaa tgatggaacg
     9061 tttacggaaa aaaatatcct ataacattcg tgacctcgtt cagattgaaa gctaccagat
     9121 ggaagatgcc aaaattgcta tttttgcagc cggaatcact tctcgcgctg ctaaagcggc
     9181 aattgcctct gcccgagccg aaggtgttaa agttggactt ttaagacctt taactatctg
     9241 gcccttccct gatgacgcag ttcgtaaaat gcttcgtaat gtagagacag ttattgttcc
     9301 ggaacttaat cagggtcagc tgattcacga agtgcaacgg ttgactaaag aaaaaagtga
     9361 cggcagtttg attcctatta acaaagtaag cagtgaattg attactccga atgatatcct
     9421 aaacaaaatc aaggaggtat gctaaaatgg ctaatattcc tcaacgcatt attcatgaat
     9481 acctgcgtca cgataagaaa tttccccatg tctggtgtgc cggatgcagt aacggtattg
     9541 ttttaggtgc cattattcgt gccgttgcct ctttgaatat agaccgtaat gatattgtaa
     9601 tggtttcagg tattggctgc tcttcccgga tgcctgttta tgtggatttt aatacacttc
     9661 ataccttaca tggacgcagt atcgcttttg ctacaggcgt aaaaatgcac aaaccgcatt
     9721 tgaaagtaat cgttgtaact ggagatggcg attgtgccgc tattggaggt aatcatttaa
     9781 tccatgcggc gcgcagaaat atagacctga aagtgattat gatcaataat aacatttatg
     9841 gaatgacagg tggacaatgc agtcctacta ctcctcataa tgcttatgct actactgctc
     9901 cttatggaaa tatagaaccc gattttaata tctgcaatct ggctatgggt gcaggtgctt
     9961 cttttgttgc cagaacaaca gctttccatg ttctggaaat gcaatcaatg ataaaagacg
    10021 ctcttcagca tccaggtttt tctttaattg aagtggtaag tgcctgtcct gtaatctacg
    10081 gacgcctgaa caaaaaaggc ggcgcagctc aaatgctgaa ggacttccgc gataattctc
    10141 ttcctcttgc cgctgtggaa aaactgcctc cggaaaaagt ggaaggaaaa atcattcgcg
    10201 gtattttacg caaagaaatc cgtcccgagt tcgctgccga atatgcagct ctgtgcaaaa
    10261 gagtgcaaac cgagggaggt gaataatggc taaaacattt gaagttcgcc tttccggaag
    10321 tggtggtcag ggcttaatcc tggcaggaat cattttggct aaagcagctg ttttagaaaa
    10381 cagaaaagta actcagacgc aaagttacgg tcctgaatcc cgaggcggtt attcgcatgc
    10441 cgatgtgatt attagtgata aggaaatata ctttcctgaa gcaacgaata ttaattgcct
    10501 gctggcatta actcaggaag catgcgataa gtttcttttt gacttgtgcg aaaacggaat
    10561 cctgattatt gatactacct tcgtaaaaaa tctggcttta gctgctgaga atacctacga
    10621 aatccctttc acggaaattg cccaggaaaa attaggcagc cctatttcta ccaatattat
    10681 ggcacttgcc ttcctggtaa aaattacagg tatcgtaaaa gaaagctctc taaaaaaagc
    10741 aatagaggaa acggtcaagc ccgcatttgt agaccttaat ttgaaagcaa tgcagattgg
    10801 ctttgacctg gcaaaaaaat agaaaaaata atgtgtagtc ggaggacgct gttcctccac
    10861 aaaaagcaga aagataaaat ccgaagcgaa tatctgataa aaaatgtgga gcttcagcga
    10921 gctccggcta cacgactata acaatcatag agttaggagt cgtcactgcc gcaagtaatt
    10981 atgcgcaact cacaaagtta tttggcagtg aggacttatg acgactctta ctaccatttc
    11041 ttaaaggaga taacttatgc ttaaacacat cagccatatt ggcatcgcag ttaaagacct
    11101 ggaagctgga attgctttct acgaaaaatt gggcttgaaa ttagaaggca ttgaagaagt
    11161 tccctctcaa aaggtgaaag ttgccttctt tccttgtgga gaaacaagaa ttgaacttct
    11221 ctgccctact tcggaggaaa gtcctattgc caaattcatt gaaaagaaag gagagggaat
    11281 tcagcatatt gcctttgctg ttgatgatat tgaagcttct ttaaaacaag caaatgaatt
    11341 aggtttagcc ctaattgata aagaacctcg tccgggagcg catcactccg atattgcatt
    11401 cctgcatcca aaatccgctt tgggagtcct gatagaattt tgccaggaac acgggtaaaa
    11461 gtgaacggat taacaggtga aacacgcgtt ggaaatagag aattagtaag ataatgagat
    11521 ggtaagaatt ataaaagatt tttcttaata tataaatcaa atcctgtaaa tcctcaaaat
    11581 cctgttcatc ccctacctat tcttttatcc gtgtaaatcc ctgtccttat cttttatcag
    11641 tgttatccgt gttatctgtg agagacattt cttttgcgtg aaatccttga cagatttccc
    11701 ctctccaaaa atctgcactc atatatacta ttataggaga aaaaatgtac gccatcgtag
    11761 aaattaaagg atttcagtat agggctgaaa aaaacgctgt gctgcgcgtt ccctatctga
    11821 aaacggcaga accgggtcaa acattaacat ttgacaaagt tcttctcctg cgtgaggaag
    11881 aagaaataaa agtcggtcaa cccgttgttg aaggcgctag catagttgca gaagtgattg
    11941 aaaatggtaa gggagaaaaa aagattattt accatcataa acgtaggaaa ggttatcgcg
    12001 tgaaaaaagg ttatcgcgaa agttacacag acctccgggt cacggatatt ttggcttaaa
    12061 aaaaagaggt taaaaatggc acataaaaaa ggtgtgggaa gttccagaaa tggccgtaac
    12121 agtaatccca agtatcgtgg tgtaaaaaaa cacgccagtg aaagtgtttt agctggtaat
    12181 attatagttc gccaaaaagg tacaaaaata caccccggca aaaatgttgg gatcggcagg
    12241 gattatacta tttatagttt aatagacggt aaggttactt ttactattgg aaaagaaggc
    12301 agaaagtatg tgagtgtgca accgatgaat gaagaatgaa aaatgtagaa tgtaaaatgg
    12361 attttgtaag gaaattataa ataactttcg gtgatttcgg tgactaacat aaaagcataa
    12421 ctgctaatta aattcggtgt tctcggtaat ttcggtgttc tcggtgtaaa tgaaactata
    12481 aggagaaaag atggaagcac aacaaattct tctgcttata cttagtatca taatcatcgg
    12541 aactaccatt atcgtaggaa tcactttata taaggaccaa gcatacacag ccaataaaac
    12601 tgctcttgtt gcagaagcca aaaattttgg taagagaatt accaaatatt gtcaagacct
    12661 tgcttcttta aagaatgata atttacaaag tgcttctgta gatacaacta agctgataaa
    12721 gtatcttggc tgggaaggca atttcataaa gacagaagca ggaactttta acattactgc
    12781 cgtttctgac tcttccgtaa ttattacggg atatgcaaaa gccaaaaaga acggcaaaag
    12841 ccctaaagtt attgttactg tcactttccc ggaaggaaaa atggatctgg tggaaagtga
    12901 ctcagtaacg aagtaaaagc aaaaagggtg attgtgcttg acataaaaag gcaatctcaa
    12961 gagttgaaca aaataaaaaa agtccggttt tggaggtaat aatggaaatc cgggatgatt
    13021 tatattacac ggacacccac gaatggatac atttagatgg cgatatagct acaatcggta
    13081 tcagtgattt tgctcagcat gagcttggcg atattgtttt tgtagagctt cccgaagtag
    13141 gacaaaaagt agcagccagt gaaccctgtg gttcaattga agccgtgaaa gcggtagaag
    13201 acctgatcag tcccctaagt ggcaaagttg aagagaaaaa cattgacctg gaagacagtc
    13261 ctgaactgat caataaatcg ccttacgaag aagggtggtt attcaaaatt cggatgtcca
    13321 atccggaaga actggaaaat ctgctttctg ctgcagagta tcagaaaata attccctaat
    13381 cccttcagag tataatatca ccttgtgatt acgaaagaca atcactttct tataatactt
    13441 tctgctcctt ccggaggagg aaaaagtact atcctaaacg aaatcctgaa agttactaac
    13501 gatatagatt attccgtttc ttacaccacg cgcagtccaa gaggaacaga acaaaatgga
    13561 attcattatc actttgtaac cgaagaggag tttcagagac gcattgacga aggtgacttt
    13621 ttagagtatg caaaggtttt cggaaactgg tatggcactt ccaaaagttt tattaaagac
    13681 cgtttagcaa aaaagcatca tgttattctg gacattgatg ttcagggtgc ttctttaatc
    13741 agtgccacgg atattcccta tgtaaaaata tttattttgc caccttctat ggaagtgttg
    13801 catcagcgtt tagttttaag agctacggat agtgaagagg aaattgccaa gcgactgcaa
    13861 attgccaaag acgaagttgc ttatatacct caatacgact atcttgtaat taacgataaa
    13921 ttggatatag cggttcagga tgttttagct attattcgag ctgaagaaaa tcgtgtatgc
    13981 cgctataatc atcccgctat ggattttata tctacaggag aaaaagatga ttaacgaaaa
    14041 acttgagagc tttctggaaa aagcggatat gaactatttg ttctgtcttt tagctcaatt
    14101 ggaagcatac cgcttacttc agcttccaac ttatgtgaaa caaaaatttc aagataaaat
    14161 tacagttatg gctatgaacc atgtagctga aaatgaagtg ccggattata ttaccgaact
    14221 tgctgaagca gagcttgcag aagcgcagga aagtcctttc caggaagaag aaggagccga
    14281 agaagaagcg gaagaaacaa ttgatgattc taaaaagttc attgaagagc ttactggaac
    14341 cgaagattat gaagaggaag aagaggaaga agaagaattc tataatgaag ataacgaaga
    14401 caacgacgaa agttaaatta tgtctgtaaa tgctttgcgc caatatctgg aattcttaaa
    14461 aaacagcggt atcaaggaac tttattatcc cccaagcgat aaacaaaaaa aattaacaga
    14521 actgcaaaaa caatattcta cctgcacaaa atgccctctg cataattcac gccataaatt
    14581 tgtttacggg gaaggaaatc ctaatgccat tgcaatgttg attggagaag gtcccggcga
    14641 acaggaagac attacaggaa gaccttttgt gggtgctgca ggtcagcttt tggaaaaaat
    14701 gctctctgct attaatctga aacgcagtga tgtttatatt accaatattg taaaatgtcg
    14761 gcctccggga aaccgaaatc ccgaatccga ggaaagaaaa gcttgtttac cctatttatt
    14821 ggaacagatt caaattattc aacctaaatt actattaatt ttaggtttgg tggcagcgca
    14881 gaccttgctg aataataata atactcttgg ctggcataga ggaaaaattc attatgttat
    14941 ggatattcct gcttttgtaa cctaccatcc ttctgctttg ttaagaaatc aggaatggaa
    15001 aaaacctgcc tggattgacc tccaggaatt ccagaaagaa tatgaaaaat tgcttgctgc
    15061 aactattagc taaaggattg ccgaattata ctgcacaaat aatgtatatc gttttatgat
    15121 tgaacataca gattcctgat tacgcgggaa taacaaggat gcttaatgaa agagggaaga
    15181 aaaaagcaat tattttattc tcacaaggag ataacgatga ttaaacagag aaattcccct
    15241 ctctctattt tcaaactgaa tttacctata tctcttctta taacttttct tttaactttt
    15301 ttagttgtag gtttggatgc ccgttttgct atttatggag atacacgcag taatccaaaa
    15361 attcatcagc aaattgttca acaaattgta tcccgcaagc ccgaggcagt ttttcatacg
    15421 ggggatttga acagttcagg caaaacgcaa aaagagtatg acaatttttt gcagattatt
    15481 tctcccatct ctcaatctgc ccgttttttt ccagcgcgcg gcaatcacga aaaagacctt
    15541 actttatttc tgcagaattt tccttattta aacggcagtt cttattacac cgtttttcag
    15601 gatggcatcc gttttatcat tctggatagt gttttagacc tttccccggg ttccgaacaa
    15661 tttagctggc tgcaaaaaca gcttaaagaa gatatccctt cactgcttat tttacatcat
    15721 ccggtttttt catccggaga gcataatgac gagcttggtt tacagctttt tttgccggaa
    15781 ttattgcaaa atacatccgt aaaagccgtt tttagcgctc atgatcataa ttacgagcgt
    15841 tccgaattta aaggtataac ttacattgtt accgggggtg gaggagctcc tttaagggaa
    15901 aaggttcacg aaaatcccta ttctttggtc ttcgtaaaaa cccatcacta tcttattgga
    15961 gagcgagaag ataagtccat tatctttaag gtctttaact tagataatga gctcatagat
    16021 agctttactt taaacggaat gtaatatgcc ccatccaaag gttttagcaa ttcacgatct
    16081 ttccggtttt ggtaacactt ccttaatggc aattatccct attatgaaca ggatgggaat
    16141 tgaagttacc gctatgccca gtgctttact ttctgccaat accgattatc ctgattatat
    16201 tctgcaggat aatagcgagc ttttaaaaaa atccctaacg cactggaaag aacttcagct
    16261 tagctttgat gccgtttata ccggtttttt gggctctcct gctcaagtat atctcttact
    16321 tgaaaatttg ccttctctgc ttaaaaaaga gggaatcgtt ttagttgacc ccgttttagc
    16381 ggatgaggga aaattatata gttgttacac ttgggaaatg gtagaggcaa tgcgttctct
    16441 tgtttcaatc agtcatatta ttactcccaa ttggacagaa gcaatttttt taagcggtta
    16501 taaaaatagg agggacttaa aaaccatcca ggtatatgaa tgctgtaaaa aattagccgc
    16561 tttgggacct caaaatatag ttataaccag tgctcctgat tctcaaggaa atttttcctc
    16621 ggttgccttt tttgctgctg aaaaaaattt tttccgggaa tttgaatgta actatgctcc
    16681 cgtaatcttc ccaggagcag gtgattgctt ttctgcattg cttttagccg gtattttaaa
    16741 tggctgtgat attgttcaat caatagaagg aactatcgcc tttttgcagg aggcaataac
    16801 aattagcatc cctgttgtgc aggatcgaaa aatgggcatt gaccttattt ctgccttaaa
    16861 aaaagacccc cacctcttct attctcaaaa agagtaaccg cgtttctttt ctacaaagag
    16921 aaaagagaaa aagggtttag caggtttaga gggtttatca ggtttaaaag gtttagaagg
    16981 ttgagagggt ttagaaggtt tagaaggttg agagggttta gataccttaa agcccggagg
    17041 gcgacacaat gatagcgatg ggtgcgtaag cccc
//
LOCUS       DAOOZV010000039        16840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3470_length_16840_cov_63.334823, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000039 DAOOZV010000000
VERSION     DAOOZV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3470_length_16840_cov_63.334823"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(659..1417)
                     /locus_tag="PLW08_05205"
     CDS             complement(659..1417)
                     /locus_tag="PLW08_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPL52037.1"
                     /translation="MKISACVFPVLEKIDQAIASMHRYIEEANAQKSDLIIFPEAALG
                     GLNITGIYTQDSQNCLAMDSSEVKSLQQKAIQYSIGIGFGFLEKEKGCIYDSFVLFDK
                     FGQIAVHYHRISRGWLPSEVTSNDYACGNIPGIADTIYGKIGVLICGDLFEPEILNCL
                     IQVETDFYFHIMARSFPCRNNFQKNWDEEEFPFYLNKYQKLGKPIMVCNCVEENAKEE
                     ERYCGGAWFIKGNRIINRMPLLQTGLLNVELLSE"
     gene            complement(1589..2026)
                     /gene="dtd"
                     /locus_tag="PLW08_05210"
     CDS             complement(1589..2026)
                     /gene="dtd"
                     /locus_tag="PLW08_05210"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPL52038.1"
                     /translation="MRITLQRVSKAICYVDNEIVGKIENGLLIFVGFATNDTFEILPL
                     AVRKCLELRIFNDENDKMNKSVLDIQGSVLVISQFTLYANCKRGRRPDFTQSAPPEKA
                     KELYEEFVHLFKQNNINVQTGIFAAKMQIELVNEGPVTINLEF"
     gene            complement(2026..2634)
                     /locus_tag="PLW08_05215"
     CDS             complement(2026..2634)
                     /locus_tag="PLW08_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52039.1"
                     /translation="MTKRLFGPCLLVLLLIFIGSCSTVTTLSFTERDVKDSPNLVKNN
                     SFSPYSTIGEEALKGWMVILNPKETGESPVVIDPEVALEGKTSLRIDASDNSVLILSE
                     PFNVRRYGGYYLRCYFKTNSPHPPRPQLRFIVFKENGKIVNRFKIKAKVNEDWERATI
                     SAGFIKPGARFGRLGIYIPPFKEGSIWIDDTGCFEVHGFKID"
     gene            complement(2631..3851)
                     /gene="rodA"
                     /locus_tag="PLW08_05220"
     CDS             complement(2631..3851)
                     /gene="rodA"
                     /locus_tag="PLW08_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPL52040.1"
                     /translation="MFINRKKFDFVLFTLLLLLIIFGCVIIFSASTTVIGGQIHTENN
                     WWKQIIFAFLSLGVILLLLRLPMPIFDLLILPLYILNILALILVFFTPAVSGAHRWFS
                     LGGINLQPSESAKLLTILLVAKVISKENLNEYKQILYGFLLTLLPALLIILEPDFGTT
                     LVFWASLIAMLIAADVPLYFILLIISPVVSVICSVWLPAIPFWIAILVILLLKSHLSW
                     VAITVSGIINVFIALITPVFWMGLKDYQQNRILTFMDPTRDPLGAGYQIIQAKIAIGS
                     GSVFGKGWLKGTQKNMKFLPEHHTDFIFSVLGEEFGFIGCMLLLLLFVAFFLRLIHNL
                     GELKVRERKVATAGILAYLMFQTFINIGMNIGLVPATGIPLPFISYGGSNLLVNSIAV
                     GVVLKYLNERGFIK"
     gene            complement(3841..5754)
                     /gene="mrdA"
                     /locus_tag="PLW08_05225"
     CDS             complement(3841..5754)
                     /gene="mrdA"
                     /locus_tag="PLW08_05225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPL52041.1"
                     /translation="MIKNQTTVIFSLVLAILFLLLAVSLFRLQVVEGTKYQRIAESNF
                     VRIRRITATRGEIYDHKYRPIVQNVPSQNLFLTSGKIKNLPALSKFLEHNFGISENEL
                     KDLIFKQRFKTYEEILLADNIPYETVLTLSEELNYYPELSFRIGSTRNYLYPNHFTGY
                     VGRINEQEYELYQKEDYSLNSYIGKTGLERYYEVLLRGKDGREIVQVDAQGRSLQLFE
                     DGGYIEPLNGLSLVLTIDNDLQEFVAGIFPEGMKGAIVVSDIRSGGILAYVSKPDYDP
                     NLFMQKISPETWEYLNRAEKPMMDRIIYGTYPPGSVFKPVTAGIGLDRGLVTPETRLA
                     FCAGGLKVGNRFFRCWYSSGHGSTSIVDALRVSCDTYFYDLSLKINLDDFYNYVRDSH
                     LYGRTGIDLPNERQGFFPNTEWYRKTFGKQEGIIGHKVNLAIGQGEVLTTPLQINAFY
                     GAIANNGLWVQPHLLKQTVGRGRLTREQVIPLTKKKLPMRPETLKTIQQGLWSVVNAP
                     GGTGANARINGATIYGKTGSAENVMGRKTHAWFCGYLVTNKPEIVVTVFLENAGGGGA
                     MAAPLTAKIFNYYMGNLEIIKQIAPLPPQFRTAEGIEAEEQEQGIEDSGFLTPETETE
                     PSVSQPQEESNVH"
     gene            complement(5747..6241)
                     /gene="mreD"
                     /locus_tag="PLW08_05230"
     CDS             complement(5747..6241)
                     /gene="mreD"
                     /locus_tag="PLW08_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPL52042.1"
                     /translation="MVWKGIWSFILGLFFLYLQILVMPALAIWNVIPNILLPWLIYTV
                     WTRPRDLALIVVFIIGLMFDSVNPMTFGLYAFVFCLLALAINEFRKPFETESFVAKML
                     TIGIVNLLFSIIQLLVFGVTFSFASPLLTKSLLGFGYNVLLSLIVFWGLQLLSKVRLN
                     ISND"
     gene            complement(6241..6933)
                     /locus_tag="PLW08_05235"
     CDS             complement(6241..6933)
                     /locus_tag="PLW08_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52043.1"
                     /translation="MSKSKQTEPIITIGGVKFERIPVKTKILTPQDDIGEVIKEYALP
                     LMQPGDILTISESPLAITQGRAIPVAEIKVSPLAKLLSRFVAKVPYGIGLGAPTSMQC
                     AIEETGVLRILLAAFIGAIGKLLGRKGDFYRVAGIQAALIDAATTSPVPPYTDMVIKG
                     PVHPKEVCEDLHSRFGYEFAVMDINDIGGCWMIGSSPNVSKHFMEKVMKDNPQGQGDE
                     LTPFCIVRRVDN"
     gene            complement(6926..7690)
                     /gene="mreC"
                     /locus_tag="PLW08_05240"
     CDS             complement(6926..7690)
                     /gene="mreC"
                     /locus_tag="PLW08_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPL52044.1"
                     /translation="MLKKNLVPLILIVLSLLMLIGSNESRLKKAGWMGNTVFFPFVRS
                     LKAVETRQELKQEVFSLRKRLAETTLENLALQNELKQYTSAFSIAFDIGEADLVQAEI
                     IGISGQYQELNLIVDKGIGSGIQIDDPVISANGIVGKIIQVSSDHSIVLPFSNPRFQL
                     PVMDSRTSVQGILQADISGSIAMNLIKLGSDIAVGDTIVTSNLSRLFPKSYPVGTIKR
                     IRESQDNLFISADINPFTLVENLEHIFILKKAVKNE"
     gene            complement(7758..8795)
                     /locus_tag="PLW08_05245"
     CDS             complement(7758..8795)
                     /locus_tag="PLW08_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPL52045.1"
                     /translation="MSVFDFFGMMANDIAIDLGTANTLVYKKNGGIVINEPSVVAVST
                     DNKKIIAIGNPAKLMLGKNPDEVRVIKPLKDGVIADFQVTELMLRNLILRAQKKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGSREVHLISEPIAAAIGADLPIDMACGNMIM
                     DIGGGTSEIAVISLSHIVVQNSIRVGGDKIDNDIINYLRKKSNLYVGVQTAEKIKTTI
                     GSAYPLKQELTMDVRGRDIVSGYPVTIKITSEEIREAISETVATIIDAIKRLFERTAP
                     ELSADIAERGIFLTGGGALLKGLDEKIRKTVDLPVHVVPDALECVVKGAGKVLDDIDR
                     YRQVLIKRIED"
     gene            complement(9257..11782)
                     /locus_tag="PLW08_05250"
     CDS             complement(9257..11782)
                     /locus_tag="PLW08_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52046.1"
                     /translation="MRRIRFLIFALLLIMLSPIYGKHSDKYLLANYSYFRCHQNIPDF
                     YYTLLDSMQTAYYNASVITFLPGDFPQRSDLLLKEMDKHKLDVLLYDMAFTEGSKNPE
                     YGSEAFSIANYWRFEAEYDSTFRENLLDDMYFYNNSYTTGTPVFNELASGKYLLQLKN
                     GQPGYAFNKLEFRWQEKGYTKYNIGNEFRFIQREMIDGRKGDIKDNPAGDDTLYITIA
                     FKCSNIPDDPNTELMRFSFNGFDKANKECQVRHYNTYNSRYGTVSYLTVGEYNKLPLI
                     TKDQEGNSVWQHKEIVLKISLQDLYYAGLLDSNIIWKYLLINLNPQIYWNGKGILELD
                     YVEFEDTMYKRQKTDTELIEAVRNRMYNLCSRYDNIKYFYLTDEPTQGQFSSFRRLEK
                     DIFKDLKTFAPSCSGFYTCSILHRKNVIKPNNMMYDHIGLYSKIVEPELIAFDIYPLR
                     GWMQWNNPTERRGVQRRLDYDMLDHYKHYKELCMKTGAKYMPCPQSYGEWNYTDAENG
                     YWALLRPPKYMQKCLQLLPLCYGADGILTYRIYNRIYEENVMKLGFQEFTTIDVSPSG
                     KVTFRPGWEGLKEANQKIIFYADYLEPREWLDADAILTTGYRNPEQLKSVMVKAIEVL
                     PQKLVQNDVDLYDGYVQCGLFKAEGKYPYFMLVNRRTEYISMVNNPTRSDSLLNVPPD
                     KLDDHFLPAPPQTVKFSIDNSAKEILGKYVALYDPFSEELFYSDSNTPEISIDPGDGR
                     LLQMCATLPKKVEGKAEMNHLAILQGNITLEKKADVTVKSDCKLIIKDNSKITVKKGA
                     KLNIFGEVEIGDNVQIQVHKGGTIYLNETDCKWGKNSKISGEK"
     assembly_gap    12372..12471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12499..13395)
                     /locus_tag="PLW08_05255"
     CDS             complement(12499..13395)
                     /locus_tag="PLW08_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPL52047.1"
                     /translation="MEKTILIIYLLLGLLLFSCTKKTDTEIPEDKVEPEQMEKTEEKT
                     ESKSVSALDMTAIESYKYTWSNNTDAMPPVVIIIDDFGQNAGQLLDDFSALPKEIAFS
                     ILPDLPYTQTVARLAEKTNHETLIHIPMQALDHQANPGKRYIRTGMDKYAISDLLQDF
                     YAQIPNAVAANNHMGSEVTSDLATMNIILEELDNLGLYFLDSATTNKSAAFTAGKNLG
                     LKIAKRDIFLDVPDNSDATIISKIEGLAKYKGRNEPVVIITHCHNRDKLNALQKFLKQ
                     IDNMEIELISVSQLFRKLGYPA"
     gene            complement(13377..13868)
                     /locus_tag="PLW08_05260"
     CDS             complement(13377..13868)
                     /locus_tag="PLW08_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPL52048.1"
                     /translation="MPKRILIILGLIGICFLFSCKKTASPTPEPTPKYEFRQDGTLEI
                     ISPEGITKATFAIEIAEKEEELMRGLKYRETMAENQGMLFIFEFPDIYDFWMQDTYLP
                     LDMLFIASDGTINYIYEKAIPFSENRITPSSLHKYVLEVNAGTVKKFNIKTGDKIKWK
                     RQS"
     gene            complement(13880..>15496)
                     /locus_tag="PLW08_05265"
     CDS             complement(13880..>15496)
                     /locus_tag="PLW08_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPL52049.1"
                     /translation="PQTANITVPTTGYITQNFTLQPAMLTTFEGQIRTPAGNTLCGAS
                     ITLKTNPPITVQSDIEGLFSFNVYEGDYQIDISALDNATYTYPVQIRQNDKRNIFIMQ
                     NSLFSDNFENGLGNWTATGTWGIVTYNDSNVLTDSPTGNYGNMQNNSITLTNPISLIR
                     VSNPYLSFKCKYALENSYDFVYVEASANGSNWIILDSFTGTVNNWINQSYSLASFAGG
                     QLYLRFRIKTDWGQTADGIYIDDVYIIGINNNIALYGDVTSEGIINMQDIAFINEYAI
                     GLDPIPEIDLRPWEAYRITNADVDGNGTIDAFDSYLLCKYIYVPDYSLPVQTGIPEEV
                     PVPVLTASYNNNLNLNFSNIDELKSLTVSVAPNYINQVHHLGIYTNQPFVQAINYETG
                     TYGYAGYEIEQQSISITLEPNPQDFTLYYTVNGVLGSQFVNIGNAADDPNAPETVTYL
                     LPNSPNPFNPETTLSFMLAKNNTLVALNIYNLKGQLVRHLVSCILPSGKHSFVWNGLD
                     DTGKEVSSGVYLYRLSTPDYQQTRKMLLAK"
     assembly_gap    15497..15596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15597..16631)
                     /locus_tag="PLW08_05270"
     CDS             complement(<15597..16631)
                     /locus_tag="PLW08_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPL52050.1"
                     /translation="MKRVFFTLCLLILATAVLAYPLHIQSWNLQEDIKKLNELRVSID
                     YVNLQTGIIHIEVRNDAERDKIHSAGMLTELLPNPAEEYFASLAEGIKDGRSYYTLTE
                     YQNFMQQTADQYPNICQLVQFGTSVQNRPLLMLKISDNVTIEENEPELKYFGSIHGDE
                     VVGYDMLIRLIQLLTTQYGIDPRITNMVNNTEIWINPMLNPDGYAAGVRYNANGIDLN
                     RNFPMPTGVQHPDGGPWAAETIAVMDFSNAHDFDLALNFHGGSLVINYPWDYTTTLTP
                     DNDLLIEMALTYSRENLPMYNSTEFLHGIVNGAAWYIVTGSMQDWNYHYTDCIEMTAE
                     ISNNKWPPAST"
     gene            complement(16754..>16840)
                     /gene="ffs"
                     /locus_tag="PLW08_05275"
     ncRNA           complement(16754..>16840)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW08_05275"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         5233 a         3366 c         2875 g         5166 t
ORIGIN      
        1 acctattaga attgagaatt gaaaattaaa aaaggttgag aaggttgaga gggttgagat
       61 ggtttagttc ttgttttaac tcacaatttt tagattggga aaccatttat tgcacggttg
      121 gaagtgtaga agcaagagaa gtggtgataa ttgggagaac aggtgaaaat tgtgagtcaa
      181 aactttctcc tcggtaaaaa ttgtgagtta aaatagaata tgcgctgggg ttaaaacctg
      241 tcgttatcta actgccatca ctacaggatt tatcttatta tataaagttt atgaatatta
      301 aaactatata aaatatggag ttacagatat aatattttct tcttccccct aaattttcct
      361 aactcctact aaataaatat aattccattt tcttccttaa tagcattgct tactcattgc
      421 ttacccattg ctaatcccat ccgtaatggg taagcaatgc ttcagcaatt caagctggga
      481 aaagctctga aaacaatgga ctttatttaa cccccaaaca tcaacccaac ctttaggatg
      541 ggtggataat ggataacaat taagaacggt gtaataaatt gtgtagatgg agctccccaa
      601 aatcacaaac ttttttttct tggggaggaa cggtgtcctc cagctacatt tttgcggttt
      661 attcagacaa caattctaca tttaacagcc cggtttgtaa cagaggcatt ctgttaataa
      721 tccgatttcc tttaataaac caggcgcctc cacaatatct ttcttcttct ttggcatttt
      781 cctcaacgca attgcagacc ataatcggtt taccaagttt ttgatattta tttagataga
      841 agggaaattc ttcttcgtcc caatttttct gaaagttatt cctgcaggga aaactccttg
      901 ccataatatg gaaataaaaa tccgtttcta cctggataag acaattaagt atttccggtt
      961 caaacaaatc cccgcagatt aaaactccga ttttaccgta aatagtatct gcaatccccg
     1021 gaatatttcc gcaagcatag tcgtttgagg taacttctga aggtagccaa cccctggata
     1081 ttctgtgata atgaacggca atctgaccaa atttgtcaaa caagacaaag ctgtcataaa
     1141 tacaaccctt ttctttttcc aaaaagccaa aaccgattcc tatggagtat tgtattgctt
     1201 tttgctgtaa ggattttact tcagaagaat ccattgcaag gcaattttgg ctatcctgag
     1261 tgtagattcc tgtaatgttc aagcccccta aagcggcttc aggaaaaata atcaggtcgc
     1321 ttttttgcgc gtttgcctct tctatatagc gatgcattga tgcaattgcc tggtctattt
     1381 tttctaggac aggaaaaaca caggcggata ttttcatttt gttgttctcc tttttggcga
     1441 aggaacagcg tcctccagct acactttgtt tttgttttag ctcacaattt ttttaatcag
     1501 gaaatcagtg caagctcggt tgtaaaattt cacgaggggc taacgccacc ttctgttctt
     1561 cattctcttt tctctttgta aaaaaaactc agaattcaag gttgatagta accggacctt
     1621 catttacaag ttctatctgc atttttgcag caaaaatacc tgtttggaca ttaatattgt
     1681 tctgtttaaa cagatgcaca aattcctcat agagttcttt tgctttttcc ggaggagcag
     1741 attgggtaaa atcgggtctg cgtccccttt tacaattagc atagagggta aattgagaaa
     1801 taactaaaac cgaaccctga atatctaaaa cacttttatt cattttgtca ttttcatcat
     1861 taaatatacg cagttccaaa catttccgca cagcaagagg taaaatttca aaggtatcgt
     1921 tagtggcaaa accgacaaaa attagaagac cgttctctat tttgccgaca atttcgttat
     1981 ctacatagca gattgcttta ctaactcttt gcagggtaat acgcatcagt caattttaaa
     2041 gccatgcact tcaaagcagc cggtatcgtc aatccagata gacccttctt taaaaggagg
     2101 aatataaatt ccaaggcgtc caaaacgggc acctggttta ataaaaccgg cactaattgt
     2161 agcgcgttcc cagtcctcgt ttactttggc ttttattttg aaacgattga caattttgcc
     2221 attttcttta aagacaataa agcgtaactg aggtcgtggc ggatgaggag aattagtttt
     2281 aaaataacat cttaaataat aacccccata gcgacgcaca ttaaatggct cacttaaaat
     2341 caaaacggaa ttatcggaag catcaatccg cagagatgtt ttcccttcca aagccacttc
     2401 aggatcaata actacagggc tttccccggt ctccttggga ttaagaataa ccatccaacc
     2461 ctttaaagct tcttctccaa tagtggaata gggactgaaa ctattattct taaccaggtt
     2521 tgggctatct ttcacatccc gttcggtgaa acttaaagta gtaactgtag aacaggagcc
     2581 gataaaaatt aacagcaaga ccaataaaca aggaccaaat aaacgcttag tcatttaata
     2641 aaaccccttt cgtttaaata ttttagaaca actcccacgg caatagaatt tactaataaa
     2701 ttagaacctc cataacttat gaacggtaac ggaatccccg tggctggaac cagaccaata
     2761 ttcattccaa tattgatgaa ggtctgaaac ataagataag ccaaaatacc tgccgtggca
     2821 acttttcttt cgcgcacctt caattccccg agattatgaa taagacgcaa gaaaaaagcc
     2881 acaaacaaca acagtaaaag catacaaccg ataaaaccga attcttcccc caacacggaa
     2941 aaaataaaat ccgtatgatg ttccggtaaa aatttcatat tcttttgcgt tcctttcagc
     3001 caacctttgc caaaaacaga accgctacca atagcaattt tggcttgaat aatttgataa
     3061 cccgctccta aaggatcgcg cgtggggtcc ataaaagtta aaatccgatt ttgttgataa
     3121 tccttcaatc ccatccaaaa cacaggtgtt atcaaagcta taaaaacatt gatgatgccc
     3181 gaaacagtaa tcgctaccca ggaaagatgg gattttaata gtaaaataac caaaatcgca
     3241 atccaaaaag gaatggcagg caaccaaaca gaacagatca cacttacaac aggactgatt
     3301 atcaaaagaa taaaatatag cggaacatcc gctgcaatca acattgcaat tagcgatgcc
     3361 caaaaaacaa gagttgtgcc aaaatcaggt tcaagaataa taagcaaagc aggcaataga
     3421 gttaacaaaa agccataaag tatctgcttg tattcattta gattctcttt ggaaataact
     3481 ttggctacta ataaaatagt aagtaatttg gcactttccg agggctgaag atttattcct
     3541 cctaaagaaa accaacggtg agctccactc actgcaggcg tgaaaaacac taaaattaaa
     3601 gccaggatat taagaatata aagcggtaaa attagaaggt caaaaatagg catcggaagg
     3661 cgtaatagca aaagaattac tcctaaggaa aggaatgcaa agataatttg tttccaccag
     3721 ttgttttctg tgtgtatctg acccccgata accgttgtag aagcactgaa aataattaca
     3781 cagccaaaaa taatcaacag tagtaaaaga gtaaagagca caaaatcaaa cttttttctg
     3841 ttaatgaaca ttactctctt cctggggttg gcttacagaa ggttccgttt ccgtttccgg
     3901 tgttaaaaat ccgctatcct ctattccctg ttcctgttct tctgcctcaa ttccttcggc
     3961 agttctaaat tgaggaggca aaggtgcaat ctgtttaata atttctagat tacccatata
     4021 atagttaaaa atcttagctg ttagaggagc agccatagct ccacctccac ctgcattttc
     4081 taaaaaaacg gtaactacga tttccggttt atttgtaacc agatagccac aaaaccaggc
     4141 atgtgttttt ctgcccatca cattttctgc ggaaccggtt ttaccgtata ttgtggctcc
     4201 gttaattctg gcattggctc ctgttccacc tggagcattt acaacagacc ataaaccctg
     4261 ttgaattgtt ttcagggttt ccggtctcat cggtaacttc ttttttgtta aaggaataac
     4321 ctgttcgcgg gtaaggcgcc ccctgcctac agtttgtttt aataaatgtg gctgaaccca
     4381 taaaccgtta ttggctatag ctccataaaa ggcatttatc tgtaaaggtg ttgttagcac
     4441 ttctccctgg ccaattgcca aattaacttt gtgtcctata ataccttcct gcttgccaaa
     4501 ggtctttctg taccattcgg tattggggaa aaaaccctgc ctttcattag gcaaatcaat
     4561 ccctgttctt ccatacaaat gtgaatcgcg cacatagtta taaaaatcgt ccaaatttat
     4621 cttcaacgac aaatcgtaaa aatatgtatc acaggatacc cttaaagcgt caacaatact
     4681 ggtactacca tgcccactgc tataccagca acggaaaaaa cggttgccaa ctttaagtcc
     4741 tccagcacaa aaagccaaac gcgtttccgg tgtaaccaaa cccctgtcca aaccgattcc
     4801 tgcggttaca ggtttaaaaa cggaacccgg aggataagtt ccgtagatga ttctatccat
     4861 cattggcttt tctgcacgat tcagatattc ccaggtttcg ggcgaaattt tttgcataaa
     4921 gagattagga tcataatccg gcttgctaac ataagcaaga atacctccgc tgcgaatgtc
     4981 gcttacaaca attgctcctt tcattccttc gggaaaaata ccggcaacaa attcctgcag
     5041 atcattatca atagtcaaca caagagagag cccattcaag ggttctatat atcccccgtc
     5101 ttcaaaaagc tgtaaactgc gtccttgagc atctacctga acaatttccc tgccgtcttt
     5161 tccgcgtaaa agaacttcgt aatagcgttc cagacctgtt tttccgatat agctattgag
     5221 ggaataatcc tctttctgat aaagttcata ttcttgttcg ttaatccttc ctacataacc
     5281 ggtaaagtga ttaggataaa gataattgcg ggtagagcca atgcgaaagg aaagctccgg
     5341 ataataattc agttcttcac ttaaagtcag cactgtttca taggggatat tatctgccag
     5401 caagatttct tcgtaggtct taaacctctg tttaaagatg aggtctttca attcattttc
     5461 agaaatgccg aaattgtgtt ccagaaattt gcttaaagca ggtaaattct ttatcttgcc
     5521 gcttgttaaa aagagatttt gagaaggcac attttgcact ataggtctat atttatggtc
     5581 ataaatttct ccccgggtgg cagttattct tctaatacgg acaaagttgc tttccgcaat
     5641 gcgttgatat ttagtccctt caacaacctg caaacgaaaa agagatacag ccaaaagaag
     5701 aaaaagaatt gccagaacaa gggaaaagat tactgtcgtt tgatttttaa tcattgctga
     5761 tgtttaaacg gactttgctt aaaagttgca aaccccaaaa aacaattaaa gataacaaaa
     5821 cattatagcc aaaaccaagt agtgattttg ttaaaagcgg actggcaaaa ctgaaagtta
     5881 ccccgaaaac aagcaattga ataatagaga aaagcagatt gacaattcca atagtgagca
     5941 tcttggctac aaaactttcc gtctcaaagg gcttacgaaa ttcattgata gctaaagcca
     6001 acagacaaaa tacaaaggca tacaagccaa aagtcatcgg atttaccgaa tcaaacatca
     6061 gtccaataat aaaaaccaca atcaaagcaa ggtctcgcgg acgagtccaa accgtataaa
     6121 tcaaccaagg taaaagaata ttaggaataa cattccagat agctaaagca ggcattacca
     6181 gaatttggag atacaaaaag aacaaaccca gaataaaact ccaaattcct ttccaaacca
     6241 ttagttatcc actctacgca cgatgcaaaa aggagttaat tcgtcaccct gaccttgagg
     6301 attatctttc attacttttt ccataaagtg tttgctgaca tttgggctac tgccaatcat
     6361 ccaacaacca ccaatatcat taatatccat aacggcaaat tcatagccaa aacgggaatg
     6421 taagtcctca cagacctctt tgggatgcac aggaccttta ataaccatat ccgtgtaagg
     6481 tggaacagga gaagtagttg cagcatcaat taaagcggct tgtatacctg caactctgta
     6541 aaaatcaccc ttcctgccca aaagtttacc tattgctcca ataaaagctg ccaaaagaat
     6601 tctgagaaca cctgtttctt caatagcgca ttgcatactg gttggagctc ctaaaccaat
     6661 gccataagga actttggcaa caaagcgact aagcaatttt gctaaaggag aaacttttat
     6721 ttcggcaact ggaatagcac gtccctgagt aatagccaaa gggctttcac tgatagttaa
     6781 aatatctcct ggttgcatta aaggcaaggc atactcttta ataacttcac caatatcatc
     6841 ctgaggagtt aggattttgg ttttaacagg aatgcgttca aacttcactc ctccaattgt
     6901 gataatgggc tctgtttgtt tacttttact catttttaac tgcctttttc aagataaaga
     6961 tatgttccag gttttccacc aaagtaaagg gattgatatc tgcactgata aataaattat
     7021 cctgcgattc ccgaatgcgt tttattgttc caacgggata ggatttggga aaaagacgcg
     7081 aaagattgga agttacaatt gtatcaccaa ctgcaatatc cgaaccaagc ttaattaaat
     7141 tcatagcaat tgagccacta atatccgctt gtaaaattcc ctggacggaa gtgcgggaat
     7201 ccattacagg caattgaaaa cggggattgg aaaaaggaag aacaatagaa tggtctgacg
     7261 aaacctgaat tattttaccc acaataccat tagcagaaat aactggatca tcaatttgaa
     7321 ttccggagcc aattccttta tccacaatca gattaagttc ctgatattga cctgaaatgc
     7381 cgataatttc tgcctgcacc aaatccgctt caccaatatc aaaagctatg gaaaaagcac
     7441 ttgtatactg cttcaattca ttttgcagag ctaaattttc cagagtggtt tctgctaatc
     7501 ttttacgcag ggaaaaaacc tcctgtttca attcctgcct ggtttccact gctttcaaag
     7561 accttacaaa cggaaagaaa accgtattac ccatccagcc ggctttttta aggcgacttt
     7621 cattgctgcc aattagcatt aacagcgaaa ggactattaa aattagggga acaaggttct
     7681 tcttcagcat cttatatccc tattaaaata gggtattatc tttttactaa gcttatcctg
     7741 aagaggaaaa ttcgggttca atcctcaatt cttttaataa gcacttgacg gtaacggtct
     7801 atatcatcca aaacctttcc agcaccttta acaacgcatt ctaatgcatc aggaacaaca
     7861 tggacaggta aatcaactgt cttgcgtatt ttttcatcca gtccttttaa cagagcacct
     7921 ccaccggtta aaaatattcc ccgttcagca atatcggcag aaagctcggg agcagttctt
     7981 tcaaagaggc gttttatggc atcaataatt gtagctactg tttcagagat tgcttcccga
     8041 atttcttcag aagttatctt aatcgttacg gggtaaccgg atacaatatc tcttccgcgc
     8101 acatccattg ttaattcctg tttcaaagga taggcactgc caattgtggt cttaattttt
     8161 tctgcggtct gcacaccaac ataaagatta ctttttttcc gaagatagtt tataatgtcg
     8221 ttatcaattt tatccccacc tacccggata gaattttgaa caactatatg cgataaggaa
     8281 ataacggcaa tttcacttgt gccgcctcca atatccataa tcatatttcc gcaagccata
     8341 tctataggta agtcagcacc aatagctgca gcaataggtt cggaaattaa atgaacttca
     8401 cgcgaaccag catgcaaagc actatcgcgc acagctcttt tttctacttc cgttattcct
     8461 gaaggaacac aaacaatcac tcggggacga actaaaaggc gtttcttttg tgcccttaaa
     8521 ataagattgc gtaacatcag ttctgtcact tgaaaatcgg caattacacc atctttcaaa
     8581 ggtttgataa ctctcacctc atccggattt ttacccagca taagtttcgc cggattgcct
     8641 atagcaataa tctttttgtt gtcagttgaa acagcaacta ccgagggttc gttgatcaca
     8701 attccaccat ttttcttata gaccaaagta ttggctgttc ccaaatcaat tgcaatgtca
     8761 ttagccatca ttccaaaaaa atcaaatacg gacattcaag acctctctat tatctgctgg
     8821 caagcaggat gaattttaat aataacaatt ttgccattct ataccttatt ttttatttag
     8881 ttggctatgt caagtaaaaa cgatttgtta cctctcatag attttataaa ataggaatct
     8941 cacggatttc acagattgga aagaagagca tacagatttt gctgatttaa gcagattttt
     9001 ttgtagcacc gaaaacacct aaaacatagt gaaaatttcc aatattactt tcagattgaa
     9061 aatgcataaa tatttcttat cttctctgga tgcaggaatg gcaaagaaca tttccttcaa
     9121 gatatgatat aatttgcccc caaacttctt gaccttagca agtcaggagt cctcactgcc
     9181 aaaataactt tcacaccttt tattaacaca tttggcagtg aggaccaata acgacgatta
     9241 gaccttggat acccttttac ttctctccac taattttaga attcttcccc catttgcaat
     9301 cggtttcatt caaatagatc gttcccccct tgtggacttg aatttggaca ttatctccaa
     9361 tttcaacttc accgaagata ttcagtttgg cacctttttt caccgttatt ttgctattat
     9421 ctttaattat caatttgcag tccgacttaa cggttacatc cgcttttttc tccagggtta
     9481 tatttccttg taagatagcc agatggttca tttccgcttt cccttctact tttttgggca
     9541 gggttgcaca catttgcagt aggcgtccat caccaggatc aatactaatt tccggtgtat
     9601 tggaatccga ataaaacagt tcctcagaaa atggatcgta gagagctaca tattttccca
     9661 aaatttcttt agcgctgtta tctatactaa atttaacagt ttgaggtggt gctggcagaa
     9721 aatggtcatc cagtttatcg ggaggaacat tcagcaaact atcggagcga gttgggttat
     9781 tgaccataga aatatattcc gttctgcggt tgaccaacat aaaataggga tatttacctt
     9841 ctgctttaaa gagaccacat tgcacatagc catcgtaaag atcaacatca ttttgcacaa
     9901 gtttttgagg tagaacttca attgccttta ccatcacaga tttcagttgt tcaggatttc
     9961 tatatccggt agttagaatg gcatctgcat caagccattc ccggggttca aggtaatcag
    10021 cataaaaaat tattttttga tttgcttctt ttaagccctc ccaacctggg cggaaggtta
    10081 cttttcccga gggcgaaaca tctatagtag taaattcctg gaaaccgagt ttcattacat
    10141 tttcttcata aatccggtta taaattctgt aggttaaaat tccatctgct ccatagcata
    10201 gaggcaaaag ctgtaaacat ttctgcatat atttgggagg acgaagtaaa gcccaataac
    10261 cattttcggc atccgtataa ttccattctc cgtatgactg cggacagggc atatatttag
    10321 ctcctgtttt catacagagt tctttataat gtttatagtg gtctaacata tcataatcca
    10381 gtcgtctttg aactcctctt ctttccgtag ggttattcca ttgcatccat ccccggagag
    10441 gatatatatc aaaagcaatc aattcgggct ccactatttt agaatacaac ccgatatgat
    10501 catacatcat attgttcggt ttaataacat ttttccggtg caaaatggag caggtataaa
    10561 agcccgaaca actgggagca aaggttttaa ggtctttgaa aatatctttt tccagtcggc
    10621 gaaaagaact gaattgtcct tgagtgggtt catcggttaa atagaaatac tttatattgt
    10681 cataacggga acaaagatta tacattctgt ttctgacagc ttcaatcagt tccgtatctg
    10741 tcttctggcg tttatacatt gtatcttcaa actccacata atcaagctct aaaatgcctt
    10801 tcccgttcca ataaatctga ggattgaggt taatcagcaa atatttccat attatattgc
    10861 tatctaaaag ccctgcataa taaaggtctt gcagagatat tttaagcaca atttccttat
    10921 gttgccaaac agaattgcct tcctgatctt tagtgattaa gggaagttta ttatattcgc
    10981 ctacagtaag ataagaaacc gtgccatatc tggaattata agtattataa tggcgcacct
    11041 gacattcttt atttgcctta tcaaagccgt tgaaagaaaa tcgcatcagt tccgtattag
    11101 gatcatcagg tatatttgaa catttaaatg caatagtaat atataaagta tcatcgcccg
    11161 caggattatc tttaatatct cctttcctgc catcaatcat ttcacgctgt atgaatctga
    11221 attcattacc tatattatat tttgtataac ccttttcctg ccaccggaat tccagtttgt
    11281 taaaggcata acctggctgt ccgtttttta attgcaaaag gtattttccg gaagcaagct
    11341 cattaaaaac aggagtgcct gtagtgtagg aattgttata aaaatacata tcatccagca
    11401 gattttctct aaaagtggaa tcatattctg cttcaaaacg ccagtagtta gcaatggaaa
    11461 atgcttctga accgtattcc ggatttttac ttccttccgt aaatgccata tcataaagca
    11521 aaacatccaa cttatgttta tccatttcct ttagtaaaag gtcagacctt tgaggaaaat
    11581 cacccggcaa aaaagttatc acagaagcat tataatatgc cgtttgcatt gaatcaagca
    11641 aagtataata gaaatcggga atattctgat ggcatctaaa ataggaataa ttcgccagta
    11701 aatacttatc ggaatgctta ccataaatag gactgagcat tattaacagt agggcaaaaa
    11761 tcaaaaatct tatgcgtcgc atttcaacct cgttaatagc ttattattta tctgtttttc
    11821 aaacacataa acatcttata caggatacag atttaggaaa tagctgtcaa gagattattc
    11881 tgcgttcttt gcataaccac aaggaatata aatagttatt gtctttagtt acctcttttt
    11941 ttctctttcc tttgatgccc ctcatcatct ttccatcatc cacccatcct aaagtatggg
    12001 ttgatgatgg gaaattaata ggtaacaaga aagcaggaga aaaaggaaaa aataaacaaa
    12061 tgttttagga agctatgatt tctttttata gtgaaaagtc atttgctatt cctgtgttct
    12121 attgtcattc aaattgatat cttatttaga aacctgttgt ttctttggca aattggaatt
    12181 tgccaacccc ctttgttttc tacagcatta ttagggtttt attataaaat tatctttggg
    12241 gtaaatccag taaatccggt tgatcctgtt atctgcgttc tattattctt attcttaatt
    12301 ctgaattatt tctcttttct ctttgtgaaa ccaattctct ttttcaagca ggatatccaa
    12361 gtttgcggaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttctctttg
    12481 tgaaaccaat tctctttttc aagcaggata tccaagtttg cggaaaagtt gggaaacgct
    12541 tatcagttca atttccatat tatctatctg tttcaggaat ttttgtaaag cgtttagttt
    12601 atcgcgatta tggcaatggg taataattac aaccggctca tttctgccct tgtattttgc
    12661 taacccctca attttgctga taattgtagc gtcgctatta tccggaacat ccagaaagat
    12721 atctcttttg gcgattttca atcccaggtt cttgccagct gtaaaagctg cgcttttatt
    12781 ggttgtagcg ctatccagaa aatataaacc aagattatcc agttcttcca aaataatatt
    12841 cattgtagca agatcggaag taacctcact gcccatatga ttattggcgg caacagcatt
    12901 aggaatttga gcataaaaat cctgtaataa atcggaaata gcatatttat ccatccctgt
    12961 ccggatatat cttttcccag gattagcttg atgatcaagt gcctgcattg gaatatgaat
    13021 caatgtttcg tgatttgttt tctctgctaa acgagcaact gtttgcgtat agggcaaatc
    13081 cggaagaata gaaaaggcaa tttctttcgg gagagcggaa aaatcatcaa gtaactgacc
    13141 tgcgttttgg ccaaaatcat caataataat gacaactgga ggcatagcat ctgtattatt
    13201 agaccaggta tatttatagg actctatagc tgtcatatcc agcgcagaaa cggatttgct
    13261 ctctgttttt tcttctgttt tttccatctg ttcaggttca actttatctt ccggaatttc
    13321 tgtatccgtt ttttttgtgc aactgaacaa aagtaagccc aagagcaaat atatgattag
    13381 gattgtcttt tccactttat tttatctccg gttttaatat taaatttctt cactgttccg
    13441 gcgttcactt ccaaaacata cttatgcaga gaactgggtg tgattctatt ttcactgaaa
    13501 ggaattgcct tttcatagat ataattgata gtgccatcgg aagctataaa cagcatatct
    13561 aaaggcagat aagtatcctg catccaaaaa tcatagatat caggaaattc aaaaataaac
    13621 agcatacctt gattttcagc cattgtttca cgatatttta agccccgcat cagttcttct
    13681 tctttttctg caatttctat ggcaaaagtg gcttttgtaa ttccttcggg agaaattatt
    13741 tccaaagttc catcctgccg aaattcatat ttgggtgttg gctcaggagt tggtgatgcg
    13801 gtttttttac agctgaacag aaaacaaatc ccgatcagcc ctaaaattat aagaattcgc
    13861 ttgggcataa atacctttct tatttggcaa gcaacatttt acgggtttgt tgataatcgg
    13921 gagtacttaa acgatataaa tatactccgg agctaacttc ttttcctgta tcatccaagc
    13981 cgttccagac aaaactgtgt tttcctgaag gcagaataca ggaaaccaaa tgacgcacaa
    14041 gctgtccttt aagattataa atattcaacg caaccagggt attatttttt gccagcataa
    14101 aggacaaagt cgtttccgga ttgaaaggat tgggactatt gggcaaaaga taagttactg
    14161 tttcaggagc attaggatca tctgcagcat taccgatatt tacaaattgc gaacctaaca
    14221 ctccgtttac cgtataatag agagtaaaat cctggggatt aggttctaaa gttatagata
    14281 ttgattgctg ttcaatttca taaccagcat aaccataagt gcctgtttca taatttattg
    14341 cctgcacaaa tggctggttg gtgtaaattc ccaaatgatg aacctgattg atataattgg
    14401 gtgctacaga aacagttagg gacttcagtt catctatatt gctgaaattc aggtttaggt
    14461 tattattata gcttgctgtt aaaacaggaa caggcacttc ttccggaatt cctgtttgca
    14521 caggtaaaga ataatcaggg acataaatat atttacagag cagatagctg tcaaaagcat
    14581 caattgtgcc attgccatct acatcggcat tcgttatccg atatgcttcc cagggtctta
    14641 aatcaatttc tggaatggga tcaagaccaa tggcatattc attgataaag gcaatatcct
    14701 gcatattgat tataccctct gaagttacat ctccataaag ggcaatatta ttattgatcc
    14761 ctataatata aacatcatca atataaatcc catcggcagt ttgaccccaa tcagttttta
    14821 ttctgaatct aaggtataat tgccctcctg caaaagaggc aagagaatag ctttgattta
    14881 tccagttgtt tacggttccc gtaaaactat ctaaaataat ccagttgctt ccattggcag
    14941 atgcttctac atatacaaaa tcgtagctat tttccagcgc atatttacac ttgaaagaca
    15001 aataaggatt agacactctt ataagcgata tcggatttgt aagagttatg ctattgtttt
    15061 gcatattacc ataatttccc gttggagaat cggttaatac attagaatca ttgtaagtta
    15121 caattcccca agttcctgtt gcagtccagt ttcccagtcc gttttcaaag ttatcagaga
    15181 aaagcgaatt ctgcataata aagatattgc gtttgtcatt ttgcctgatt tggacaggat
    15241 aagtgtaagt agcattatcc aaggcagaaa tatcaatttg atagtcacct tcatacacat
    15301 taaaggaaaa taatccttca atatcggact ggacagtaat tggtggattg gtttttaaag
    15361 taatacttgc tccacataat gtatttcctg ccggagtacg aatctgtccc tcaaaagttg
    15421 ttagcatagc tggttgcaaa gtaaagtttt gcgtaatata accagttgtg ggaactgtaa
    15481 tattcgctgt ttgaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgta
    15601 gaagcaggag gccatttatt attgctaatt tcggcagtca tttcaataca atcggtatag
    15661 tgataattcc aatcctgcat actgccagta acaatatacc aggcagctcc attaactatt
    15721 ccgtgtaaaa actcggtgct gttatacatt ggcagatttt cgcgagaata ggttaaagcc
    15781 atttcaatga gcaaatcatt atccggagtt aaagtagtag tgtaatccca gggatagttt
    15841 ataacaaggg aacctccatg aaaattgaga gccaaatcaa aatcgtgagc gttactgaaa
    15901 tccattactg caattgtttc agcagcccag ggtcctccat ccggatgttg aactcccgta
    15961 ggcataggaa aatttctgtt caaatcaatt ccattagcat tataacgaac tcctgctgca
    16021 tagccatctg gattaagcat cggatttatc caaatctctg tgttatttac catattggtt
    16081 atccgcgggt caattccata ttgagttgta agtaattgaa ttaaacggat aagcatatcg
    16141 tagccgacaa cttcatctcc atgaatactg ccgaaatatt taagctccgg ttcgttttct
    16201 tcaatcgtta cattatcact tattttcaac ataagtaaag gtctgttttg aacactggtg
    16261 ccaaattgaa ccaattgaca gatattggga tactgatctg ccgtctgttg cataaaattc
    16321 tggtattcgg ttaaagtata ataacttcta ccatctttaa taccctctgc caaagaagca
    16381 aaatattctt ctgccggatt gggtaaaagc tctgtaagca ttcctgcact atgaatttta
    16441 tccctttccg catcattacg cacttctatg tgaataatac ctgtttgcag gtttacataa
    16501 tcaatgctga cccttagttc attaagcttt ttaatatcct cttgcaagtt ccagctctga
    16561 atgtgtaaag gatatgccaa aacagccgtg gctaaaatta gcaggcataa agtaaaaaat
    16621 acccttttca tttttttatc cttataactt cattggggaa aaataattcc tttgtcattc
    16681 ctgcgcaagc aagtaggaat ctatatctta actaataagc ataaattata ccatttttgt
    16741 ttcaatatag agaaaaagcc aggcaaacaa tgtcacataa ctggtaaagc ttaatgctgc
    16801 ttcattcctg atctgacata gttcacttgc agttattgca
//
LOCUS       DAOOZV010000040        16526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3557_length_16526_cov_67.237994, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000040 DAOOZV010000000
VERSION     DAOOZV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_16526_cov_67.237994"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLW08_05280"
     CDS             <1..201
                     /locus_tag="PLW08_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52051.1"
                     /translation="NHLNLLNLINLLNHFQFFIILFLFVFPSRHSLFLFVSYFTEKVI
                     DRTWQGKKNAPENEAACFYLMA"
     gene            368..955
                     /gene="infC"
                     /locus_tag="PLW08_05285"
     CDS             368..955
                     /gene="infC"
                     /locus_tag="PLW08_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPL52052.1"
                     /translation="MPKERINNQITAEKVRLIGPDGKQIGVISLKEALRKAENMDLDL
                     VEISPNAVPPVCRILDFSKYYFEKEKKAKEARKRQHEVEVKEIKFGPNTEEHDYNFKK
                     NNAIKFLKQHNKVKFTVRFRGRQMAHKELGYKVLDKLKEDLAHLVDIDGEPVADRNMI
                     SMVVSPKKDIDRILASETKKEETPIPAVEKSEPNT"
     gene            1048..1242
                     /gene="rpmI"
                     /locus_tag="PLW08_05290"
     CDS             1048..1242
                     /gene="rpmI"
                     /locus_tag="PLW08_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPL52053.1"
                     /translation="MPKLKTNRSAAKRFKITGTGKIVRHHAKSAHIKTKKSPKLKRYL
                     RSSEIVKKPDEKRIYRMLGL"
     gene            1258..1605
                     /gene="rplT"
                     /locus_tag="PLW08_05295"
     CDS             1258..1605
                     /gene="rplT"
                     /locus_tag="PLW08_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPL52054.1"
                     /translation="MPRTTNNVSAHRRRKKYMLQARGYYGSRSKTYRAARQTVERAMA
                     FSFAHRKQKKRQFRSLWITRINAACRMNDINYSQFINGLHKANIQINRKTLAHLAWHD
                     SPAFAKLVEIAKG"
     gene            1848..2882
                     /gene="pheS"
                     /locus_tag="PLW08_05300"
     CDS             1848..2882
                     /gene="pheS"
                     /locus_tag="PLW08_05300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPL52055.1"
                     /translation="MEEELRKLVEYAEKDIFACSSPNDLLNVKAKYLGKKSLLSSLYA
                     QMRNIPVEERPAFGNLINEAKKRIEDLLEQQNKVIKENAWKKNEAKVGTDLTMPGIFS
                     SRGGLHPLTIIRREIDEVFLGLGFEIAEGPDIDDDFHNFDALNTPLDHPSRNLADTFY
                     LEGGGLLRTHTSTVQVRVMEKYPPPIKIISPGRCYRNDKPDPSHSPVFHQVEALVVDK
                     GISLADMKDMLSQFASIMFGKDVSSRIRPHFFPFTEPSAEMDISCVVCGGTGCRVCKY
                     SGWLEMGGAGMVDPNVFDILGIDSEFYTGYAFGLGIERIAMLKYNIPDMRILYENDLR
                     MLRQFKGEFR"
     gene            2879..5434
                     /gene="pheT"
                     /locus_tag="PLW08_05305"
     CDS             2879..5434
                     /gene="pheT"
                     /locus_tag="PLW08_05305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPL52056.1"
                     /translation="MKIALSWLAKYIEIKENTKELEEILTFSGIEVEAVENIPALPET
                     VFSAKIISAEPVPETDHLKRCLVDIGNFPCPEKTEDGYLQVICGAPNCRTGMMAIIAL
                     PGSVLPEMTIAKAQIKGIDSCGMLCSEKELGISDNHSGIIELNRETPIGITANELYEL
                     PDTIFELEITPNRPDLLGYIGIARDLSAKLNCSLKMPEIKIPEAKCKAETMPLELVNK
                     VPELCPRYIARLFNKVELKESPLWLKSALIKSGLRPINNLVDITNYVMLEYGHPLHCF
                     DYDKLAALPEKPGIPAIVIRRAYQNEPIITLDGKSYLLEGQEMVIADGIKPSAIAGVM
                     GGNISAISETTKRIVLESAAFNPSSIRRTSYKHKISTDSSYRFERHLSECSAEDASIR
                     ATQLICELANAELCEVVYDSYHIPVQPLIIGIRPSRYTQVIGYELEEEKIKDYLTRLG
                     LKFIQYGNWKPGKINSLAELYCHHTEEMKQGKTEFTELPNCEHTHYYEIPPYRVDLER
                     EIDIIEEIARLDGYDKIPVKTPPQRIMDRHSYRIKKNAVDYLISRGFYETLNYSFSEP
                     DLLYKLGYEDGDSELEMITLKNPQSSNQSGMRTSLIPQLLQNLAYNLNRGEKNIKLFE
                     LGKTYHRQEALSYEPYFLAGIVTGLNKEEHWQDKASPITLFWVKGIVEELLSLWYLEN
                     WSVQETKVPFLSRTESASYYLENKELAYYGKLNSVVAEKFDIDTIELKQDIWIVQFAM
                     ENIINATRNLKTHFQEIPRFPFVTRDISFLIAEEIPYSEIIKTIAEIERNIISEVTAF
                     DEYRGKQIPEGKRSLTLRLKFRDKEKTLTDERVDYLIDLIIKKLRETWQIKMR"
     gene            5438..5761
                     /locus_tag="PLW08_05310"
     CDS             5438..5761
                     /locus_tag="PLW08_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52057.1"
                     /translation="MESNVINLQDKIQKLIDQYTLATKKVEEVEEKNRQLAEENSQLI
                     AQIQTSGKATGEQEKKYKTLQEEYNTLENRYKELQKILAGLESVAEGAIKKIDGLFAS
                     SEKET"
     gene            5764..6027
                     /locus_tag="PLW08_05315"
     CDS             5764..6027
                     /locus_tag="PLW08_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPL52058.1"
                     /translation="MQSVEVEIFGRRFRLRSDDPERTKQIANEINDQIDALSKHYDNL
                     DFTKLLLLICLQQQDEVLTLDAKNRALSSELERLNQMLSKIKI"
     gene            6259..7827
                     /gene="rny"
                     /locus_tag="PLW08_05320"
     CDS             6259..7827
                     /gene="rny"
                     /locus_tag="PLW08_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPL52059.1"
                     /translation="MTINPIIALIIGVVGGFAIALIIYFIKRNTAFKLISQSTKIAEE
                     LEKNAKIEAENAKKAALLEAREEWIKQKRLLDEEIKERQKELRLEEKKYNERLSSLDK
                     RLDSLDKKENALAEQERKLKSKEEELLNKKNEFDAIIAEQKNKLAEIAGLSREEAIAR
                     LREELISQARQVAANDARLTIEQIKLDANRKAAEILSTAIQRMAVDYVSETTVSVVSL
                     PSDEMKGRIIGREGRNIRTFEKVSGVDLIIDDTPEAVVLSCFDPVRREVARLALEKLI
                     ADGRIHPGRIEEVIAKTAKEMEENLVKIGEKAVLETNIHNISPNLIKVLGRLHYRTSY
                     GQNVLEHSIESAWICGILAAELGLDQELARRAGLLHDIGKAIDHEFDGSHAIIGANLA
                     RKNGEGKIIVNAIEAHHEEVEAISVYAALTQASDAISGARPGARREMLETYMQRLSKL
                     EEIANSIDGVSKSYAIQAGRELRIIVEPSLIDENQTALLATEIAASIEKQVQYPGQIK
                     VTVIRETRQIAMAK"
     gene            7827..8636
                     /locus_tag="PLW08_05325"
     CDS             7827..8636
                     /locus_tag="PLW08_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPL52060.1"
                     /translation="MKVLFFGDVYGKAGRQTVQLTLPELKEEFQPDFILANCENLADG
                     KGVTEKTLNQLFNAGVDAVTGGNHLWDRTESLEYIRGQNRIIKPLNYPEDAPGNPFII
                     LAKENLRLGVLCLCGQLLMPPCNNPFTALSEFIKLHFTKDAEQVSLFPLPYPVILDFH
                     CEATSEKRAMGWFADGQLSAVLGTHTHIQTADEEILPLGTAYITDIGMTGSHNSVIGV
                     KKEIIIEKFRTSVPNRFESSDSGLQVNAVCLELDSETGKALNIMRIRRNIE"
     gene            8629..9552
                     /locus_tag="PLW08_05330"
     CDS             8629..9552
                     /locus_tag="PLW08_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPL52061.1"
                     /translation="MNKIMEKVLNGKPVAQLINRTCSSLIANYGLKPQMVLIQVGDDP
                     ASTYYVQSIINSGKKLGCFCQMKILPDNISEQELLAILNEANHNPDVNGIMLQKPLPK
                     HINESKVNSAINPEKDLDCLHPLNLGKIMMEEEGFLPCTPAAVLFCMLYYGIETQGKH
                     IVILGRSNVVGKPLANILLWKRNFANATVTVCHSKTNNLAEFTKEADILITAIGKAEF
                     VTPEMVKENAIILDVGINEIKLANGESNYVGDVNYKLCYDKALAITPVPGGIGRITTS
                     VLFLNLVDACLKSRNTNKSIDEYIHLIFDGK"
     gene            9584..11992
                     /locus_tag="PLW08_05335"
     CDS             9584..11992
                     /locus_tag="PLW08_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52062.1"
                     /translation="MMQLKSAKLLFIIVMIISSVLLISGCGKSGNRFANQPPTIQITS
                     FEGWDTTYVSAGYDTTEVYSFQQRIYWHATDPDGVITGYAFRILDENNNPVPTPGYEY
                     IDLTGELTPDNLLALGTGWVIHYMTGADQNIPLDDPQARRSIWTSQKYAVINFPSADS
                     LGNPIVKFSRFEVVAIDNRGAITPHPAWRNFRTQSDRPKCMISSTKGNPNGKDVGSGL
                     KLAFSMSDTDPFITAVPYKYEFKIMKINNQGEVISGTESDWFSTTGEDNRTNRINEYL
                     LTKYTNPPLSYDYDEVTLAFKNQRTKIISRVTDLAGVISIPDAASTMIFSVRPGFRPH
                     TFIYPTKTYALTNNHFEDYGDDTTEEIKVIPRVVTEGVTRYALPLFKDFEGKYTAVYS
                     TNIKLWIRWGWWGEYGETRGETVTYGDTIKIKTTPYNKKVDVVLDSQTGQNYFSEITA
                     FDLRLDGEPYNYPPYPFSKYGFTDQNGKKWLRLPVNSPLGQTVVLTAGQLPLPPATEP
                     GEHKFEVRVVDLQDEYDPTPVDFTFYLHRYIEPANRQGVLIIDDDPVSAQVNDALITQ
                     RYQAMLEGYSGNVNVITRTENNEDIRQRAIAFSDLQKYKLVIYHTDNYEKTGNLQLDF
                     DAYSLFLMRGGNLLISHTSLLGAQLTEIANGGLRKTFVTNLGFNKIPKVSYLNNSNSP
                     FFQKAVSNMTDYNDLNLHYDVTGSPAIHPLIDLRDGLGYLSYFENGNFSGDILYKFGC
                     KPTTYPTYPPTSEQFDKYNGKTVAFRRTTSSNGKVYVFGFPLSFMKVEDTRPMMNKII
                     SELM"
     assembly_gap    12384..12483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12484..13288)
                     /locus_tag="PLW08_05340"
     CDS             complement(<12484..13288)
                     /locus_tag="PLW08_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL52063.1"
                     /translation="MKQIFPVSAGYYWSDCGAFMGVLPYPLWQTKTILDQRQRRRLNL
                     NLLLIQSGKRNILIDTGIGNRLSDKQRDIYQPSEFLLPFSLAELGLKDRDITDVIMTH
                     LHFDHAGGIITNFGTYDALTFPKAHYWIQKAEWEIAKNPDELNRSAYNFEQQLALLEK
                     QGNITIIDGEAEIEPGILLTLVGGHTIGSQIVTIDFGTDYYIYAGDIIPTLFHTSLAI
                     TSAYDVSRIQTVTAKKLIYNRLKEHNGTLILNHDTTQWQITYSELEHKDK"
     gene            complement(13285..14088)
                     /locus_tag="PLW08_05345"
     CDS             complement(13285..14088)
                     /locus_tag="PLW08_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPL52064.1"
                     /translation="MTTIDKSGESLEEIIRTFREDYKIHDWELNYEILKKPTKGIFGL
                     FAHKLALVRFQLPSSFDRAELFMQNLLQKMGISYTSLKSRTEGKTLFLEINGSPDSGF
                     LIGKNGSMLDTLQYLINRVFEDNAQIERIYLDVDGYRERQADSFLKPYLPQILQVKIT
                     GKPITLEPMPAAERRIIHRYVEQEKGLRTLTIGEGEKKRIVVFSAEQNQKEALSQSRT
                     EPKRRNNKAPIHTHKPVPTPNNKTGNTSLKVKKPNRQQKQPKVSNTKHQ"
     gene            complement(14085..15716)
                     /gene="yidC"
                     /locus_tag="PLW08_05350"
     CDS             complement(14085..15716)
                     /gene="yidC"
                     /locus_tag="PLW08_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPL52065.1"
                     /translation="MEKRTLLALLLVFILFLVFNQFVWKPQQLRQQQEQQNQQTQTPQ
                     TVTEPVKPDTIIITPMPDTLLAKEKTPEIIKLSNQLMTVTFNSRGGSIQQVELHNFIM
                     HDSTKVKLIPENSSLADVKLIHPVGETPLKDVIFQYKISPDSLGVTFFLGDETNPQIS
                     KSFALDNQYGISFAINVENYQVINGIEVDFSCGIADTETVTKSKQQDYRFMYYADNEI
                     FKTTLAKMKKKQPSGNLNSFNWMALRGKYFTLVLKENEPALMRSFRTELNPQTGNPAF
                     VIDSKQSNPKQSWSQSFIIYAGPTDYQILQKYGKQMDNIPERGASWLRWLANIFEWIL
                     KFLHRYIKNYGVVILIFAVIIKLLLHPLTQHQMVHSMRQQKMQPYIEEIRKKYANDVV
                     KQREELTKLYKENKTSMTAGCLPLLIQIPIIIPLYSVLRYSLDMRNASFCLWLKDLSE
                     PDPYMILPIIMGGFMLLQSLMMRPKNVDTSQMTEQQKMQQSQMKIMTWGMPIFMVIIF
                     RGMPAGLVLYWTAFNVLSILHQYFLTKHLKKKENK"
     gene            complement(15727..15957)
                     /gene="yidD"
                     /locus_tag="PLW08_05355"
     CDS             complement(15727..15957)
                     /gene="yidD"
                     /locus_tag="PLW08_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPL52066.1"
                     /translation="MRQILHFPNLLFLALIRFYQLAVSPFLPASCRFEPSCSVYSYEA
                     FKKYNFFKAFYLTAWRILRCNPFHKGGYDPLP"
     gene            complement(15954..16286)
                     /gene="rnpA"
                     /locus_tag="PLW08_05360"
     CDS             complement(15954..16286)
                     /gene="rnpA"
                     /locus_tag="PLW08_05360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPL52067.1"
                     /translation="MLQWITSHNEYSDFFKPDFMIRSSYFYVPGLYREGEIAVGITIS
                     NKVGKAVKRNLLKRRIKSWLRTNQDKIPVGYRFNLIARSGATELEWQELSRELTNIIA
                     QLNIQTRS"
     gene            complement(16291..16425)
                     /gene="rpmH"
                     /locus_tag="PLW08_05365"
     CDS             complement(16291..16425)
                     /gene="rpmH"
                     /locus_tag="PLW08_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPL52068.1"
                     /translation="MKRTYQPSNRSRKNTHGFRSRMATKNGRKVLARRRAKGRKTLTV
                     "
BASE COUNT         5239 a         2912 c         3351 g         4924 t
ORIGIN      
        1 aaccatctca accttctaaa ccttatcaac cttctcaacc attttcaatt cttcattatt
       61 ctatttctct ttgtttttcc atctcgccat tctcttttcc tctttgtttc atacttcaca
      121 gaaaaagtga ttgacaggac ttggcagggc aaaaaaaatg ctcccgaaaa tgaagctgct
      181 tgcttctacc ttatggcttg attattgtgc tgataaggtt taaacccata taaaaaagaa
      241 atacaaaagt atttactgtg aagcaggcgg ttttccgata aaaaagataa ccgcttgttt
      301 tgtttccggt aagaaggagg ttcttataat ccggattaag aaaggaaagg cacgcacgca
      361 tgaagttgtg cctaaagaaa gaatcaataa tcagataacg gcagaaaagg ttcgtcttat
      421 aggtccagac ggaaaacaga taggagttat atctttgaaa gaggcattgc gtaaagcaga
      481 gaatatggat ttggatttag tggaaatctc tcctaatgct gttccgccag tatgtcggat
      541 tctggatttc agcaagtatt attttgagaa ggaaaagaag gcaaaagaag cccgaaaaag
      601 acagcatgaa gtagaagtga aggaaatcaa gttcggtccc aatacggaag aacacgatta
      661 taacttcaag aaaaacaatg ctatcaagtt cttgaaacag cataataagg ttaaatttac
      721 agttcgtttc cggggcagac aaatggctca taaggaactt ggctataagg ttttggataa
      781 actgaaagaa gaccttgctc acctggtaga tattgatggt gagccggttg ctgacagaaa
      841 tatgatttca atggttgtct cacccaaaaa ggatattgac cgtatcttag ccagtgaaac
      901 caaaaaggaa gaaacaccta ttccggctgt agaaaagtcg gaaccgaata cttaaagaca
      961 tattgaaaat agaaacattc cggaagttcc ctttccgtaa tggaaccttt aataaaggga
     1021 aatgagtcac aatataagga gataaaaatg cctaagctca aaaccaaccg ttcggcagct
     1081 aaaagattta aaattactgg aacgggaaaa attgtgcgtc accatgctaa aagtgcgcac
     1141 attaaaacaa aaaaatcgcc taaactgaaa cgctatctgc gaagcagtga aatcgtcaaa
     1201 aagcccgacg aaaaaagaat ttacaggatg ctgggtttat aattaaggga ggatgaaatg
     1261 ccaagaacaa ctaataatgt atctgctcac cgcagaagaa aaaaatatat gctacaagct
     1321 cgtggctatt atggaagtag gagcaaaaca tatcgtgctg ccagacaaac agttgaacgc
     1381 gctatggctt tttcttttgc acatcgtaaa cagaaaaaac gccaatttcg gagtttgtgg
     1441 ataaccagaa ttaatgctgc ctgcaggatg aatgatatca actacagcca atttattaac
     1501 ggtctgcata aggcaaatat tcaaatcaac cgtaaaacat tggcacatct tgcctggcat
     1561 gattctccgg cgtttgctaa actggtagaa attgctaaag gttaaaaaaa ggtaatagca
     1621 cgtagattac gcaggtaaaa agcaatggga tttcaagaat gaaatagatt taagggagaa
     1681 aaaggtgttt aactatatta ctatatatgc gttaagaaaa attccgaaca tttttgggaa
     1741 tgataaaaat aacatccttt tccatcctta taatctcata aatcccttac ctaataatta
     1801 aatctgcata atcagcgaaa tctgcgtgca gattaaatga gggaaaggtg gaagaggaat
     1861 tacgaaaact cgttgaatat gcagagaaag atatttttgc ttgctccagc cccaatgacc
     1921 tattgaatgt aaaagccaaa tatttgggca aaaaaagttt gttgagctct ctttatgctc
     1981 aaatgagaaa tataccggtt gaagaacgtc ctgctttcgg caatttgata aatgaagcca
     2041 aaaaacgcat agaagacctt ttagaacaac agaataaagt catcaaagaa aatgcctgga
     2101 aaaaaaacga agccaaagta ggcaccgatc ttacaatgcc tgggattttc agttcccggg
     2161 gtggcttgca tcctttaaca attatccgtc gtgaaattga tgaggtcttt ttgggtttag
     2221 gttttgaaat tgctgaggga cctgatattg atgatgattt tcataatttt gatgctttaa
     2281 acactccttt agatcatcct tcgcgtaatc ttgccgatac tttttatctg gaaggaggag
     2341 gacttttaag aactcatact tctactgttc aagtaagggt gatggaaaaa tacccacctc
     2401 caataaagat aatttcccct ggtcgttgtt atagaaatga taagcccgat ccttctcatt
     2461 cgcctgtctt tcatcaggtt gaggcattag ttgtagataa aggtatttct ctggcagata
     2521 tgaaagatat gctgtctcaa tttgcttcaa ttatgtttgg caaagatgtt tccagcagaa
     2581 tcagaccgca ttttttcccc tttacggaac ccagtgcgga aatggatatt tcctgtgttg
     2641 tttgtggtgg aacaggttgc agggtttgca aatattccgg atggttggaa atgggtggag
     2701 cgggaatggt tgatcccaat gtttttgata tattggggat tgattccgaa ttttataccg
     2761 gttatgcttt tggcttggga attgaaagga tagcgatgct gaaatacaat attccagata
     2821 tgcgtatttt atatgaaaac gatttaagaa tgttaaggca gttcaaagga gaatttagat
     2881 gaagatagct ttgagctggc tggcaaaata tattgaaatt aaagaaaata cgaaggaact
     2941 ggaagaaata ttaacttttt cgggtataga agtagaagca gtggaaaata ttcctgcttt
     3001 accggaaacc gttttcagcg ctaaaatcat ttctgcggaa ccggttccag aaacagacca
     3061 tttgaaacgc tgtttggtag atattggtaa ctttccttgc cccgaaaaaa cggaagatgg
     3121 ctatctacaa gtgatttgcg gagcgcccaa ttgccgaacg ggtatgatgg ctataattgc
     3181 gcttccgggt tcggttttgc ctgaaatgac tattgccaaa gcccaaatta aaggcattga
     3241 ttcctgtgga atgctctgtt ccgaaaaaga attgggaatt tcagataatc atagcggtat
     3301 aattgaactt aatagagaaa ctcccatagg aataactgct aatgaacttt atgaacttcc
     3361 cgatacaatt tttgaactgg aaattacacc caaccgtcct gatcttcttg gttatattgg
     3421 tattgcgcgt gatctttccg ctaaattgaa ttgttcttta aaaatgccgg aaattaaaat
     3481 tcccgaggca aaatgcaaag cggaaacaat gcctttggaa ttggttaata aagttcccga
     3541 actttgtcca cgctatattg cccgcttatt taacaaagtg gaattgaaag aatcaccgct
     3601 ttggctgaag agcgcattga taaaatccgg acttcgtcct ataaacaacc ttgtggatat
     3661 taccaattat gtaatgctgg aatatggaca tcctttgcat tgttttgatt atgataagct
     3721 tgctgcgctt cctgaaaaac caggaatacc ggcaattgtt attcgtaggg cataccaaaa
     3781 tgagcccata attactttag acggcaaaag ctatcttttg gaaggacagg aaatggttat
     3841 tgctgatggc attaagccct ctgccatagc aggagttatg ggtggaaata tttctgccat
     3901 cagtgaaact acaaaacgca tagttttgga aagtgccgct ttcaatccga gttccattcg
     3961 gagaacttct tataagcata aaatcagcac cgattcctcc tatcgttttg aacggcattt
     4021 aagtgaatgt tctgctgaag atgcctcaat aagggctact cagctgattt gtgaactggc
     4081 aaatgcagaa ctttgcgaag ttgtttatga cagttatcac ataccagtgc aacctttaat
     4141 tataggcatt cgtcccagtc gttatactca agtgattggt tatgaactgg aggaggaaaa
     4201 gattaaagat tatctcactc gtctgggact gaaatttatc caatacggga attggaaacc
     4261 gggaaaaatt aactctcttg cggaactata ctgtcatcac actgaagaga tgaaacaggg
     4321 gaaaaccgaa tttaccgaac ttccgaattg cgagcatact cactattatg aaataccccc
     4381 ctatcgggtt gacttggaac gcgaaataga cataatcgag gaaatagctc gtttagacgg
     4441 ttatgacaaa atacctgtaa aaacccctcc acaaaggatt atggaccgtc attcctatcg
     4501 gataaagaaa aatgctgtgg attatttaat cagtcgcggc ttttacgaga cacttaatta
     4561 tagtttttcc gaaccggatt tgctctataa attgggctat gaagatggcg atagcgaact
     4621 ggaaatgata actttgaaaa atccgcaaag tagtaatcaa agcggtatgc ggacttcttt
     4681 aataccgcag ttattacaaa atttggctta taatctgaac cgtggcgaaa aaaatatcaa
     4741 actttttgag ctgggaaaga catatcacag gcaggaagcc ctctcttacg aaccctactt
     4801 tttagccgga attgtaaccg gactgaataa agaagaacat tggcaggata aagcaagccc
     4861 gattactttg ttttgggtga aaggaatcgt agaagaactg ctctcccttt ggtatctgga
     4921 aaattggtct gttcaggaaa ctaaagtgcc ttttctttcc cgcactgaaa gcgcctctta
     4981 ttatctggaa aataaggaac tggcttatta cgggaaatta aattctgtag tggcagaaaa
     5041 atttgatatt gataccattg aattaaaaca ggatatttgg atagttcaat tcgctatgga
     5101 aaacattatc aatgctactc gcaatcttaa aacacacttt caagaaattc cccgttttcc
     5161 cttcgtaaca agagatattt cttttcttat tgcggaagaa attccctaca gcgaaataat
     5221 aaaaacaatt gcggaaattg aaagaaatat aatcagcgaa gtaactgcct ttgatgaata
     5281 ccgtgggaaa caaattcctg aaggtaagag gagcttgact ttacggctga aatttagaga
     5341 caaagaaaaa acattgacag atgaacgagt agattatctt attgatttaa taataaaaaa
     5401 gctaagggag acctggcaga taaaaatgag gtaacttatg gaaagtaatg taattaacct
     5461 acaggacaag atacaaaaat taatagacca gtatactctg gcaactaaaa aagttgagga
     5521 agtggaagag aaaaatcggc aacttgcaga agaaaattct caactgattg cccaaattca
     5581 aaccagcgga aaagcaactg gtgaacagga gaaaaaatat aagacactgc aggaagaata
     5641 taataccctg gaaaacaggt ataaagaact gcagaaaatc cttgccggtt tagaaagtgt
     5701 tgctgaaggt gctattaaaa agattgacgg attattcgcc tccagcgaaa aagagacctg
     5761 ataatgcagt cggtggaagt ggaaatattt ggacgcagat ttcgtttgcg tagtgatgat
     5821 cctgagcgga caaaacaaat tgcaaacgaa attaacgacc aaatagatgc gctttccaaa
     5881 cactacgata atttggattt tactaaactg ttgcttttga tttgtcttca gcaacaggat
     5941 gaagttttaa ctttggacgc caagaatcgt gctttaagct ccgaacttga gcgtttgaat
     6001 cagatgcttt caaaaataaa gatataaaaa gtgcctgcag aattcgtgat atgggttgca
     6061 taaaagctat tatagtttcg ggtgtcgagc catgagtggc atgcgcgcca gctggtcctg
     6121 gatacataaa gctcacaagg tagataccca cttcaagctt ggctttctaa tgacaagcgt
     6181 acacggttca tgcgggcact tgaataattc gctttggtta taaatcaaag ttaaatagat
     6241 tgaaataagg aaataacaat gactataaat cctattatag cactcatcat aggagtggta
     6301 ggcggttttg ccattgctct tattatatat ttcataaagc gtaatactgc tttcaaactg
     6361 atttcgcaat caactaaaat tgccgaagag ttagaaaaga acgctaaaat tgaagccgaa
     6421 aatgctaaaa aagcagctct actggaagca cgcgaagaat ggattaaaca aaaacggctt
     6481 ctggatgagg aaattaaaga acgccaaaaa gaactgcgcc tggaagaaaa aaaatacaat
     6541 gaacgtttaa gcagtttgga taaacgcctg gactctctgg ataaaaaaga aaatgccctt
     6601 gcggaacagg aaagaaaatt aaaaagcaaa gaagaagaac tgctcaataa gaaaaatgaa
     6661 tttgatgcta tcattgctga acaaaaaaat aagctggctg aaattgcagg gctcagtcgt
     6721 gaagaagcaa ttgcccgttt gcgggaagaa ttaatttcgc aggcaaggca ggttgcggca
     6781 aatgatgccc ggcttactat tgaacaaata aaactggatg ccaacagaaa agctgcagaa
     6841 attctttcca ctgctattca acggatggca gtagattatg tatccgaaac aaccgtctct
     6901 gttgtatcgc ttccttccga tgaaatgaag ggaagaataa ttggcaggga aggaagaaat
     6961 atccgcactt ttgaaaaggt ctccggagtg gacttaatta ttgatgatac tcccgaggca
     7021 gttgtgctca gctgttttga tcctgtccgt cgtgaagtag ccagattagc tttggaaaaa
     7081 cttattgccg atggtagaat tcatcctggt cgcattgaag aagtaatagc caaaacagct
     7141 aaggaaatgg aagagaattt ggttaagatt ggagaaaaag cagttctgga aaccaatata
     7201 cacaatattt caccgaacct cattaaggtt ttggggcgtt tgcattaccg aaccagctac
     7261 ggacaaaatg ttttagagca ctcaatagag tccgcttgga tttgtggaat tttagctgct
     7321 gagctgggtc tggatcaaga acttgctcgt agagcaggtt tattgcatga tataggaaaa
     7381 gctatagacc acgaatttga cggttcccac gctattattg gagccaattt ggcacgcaaa
     7441 aacggtgagg gaaaaataat tgtgaatgct attgaagctc accatgaaga agtggaggca
     7501 atttctgttt atgccgcttt aactcaagca tcggatgcta tttctggtgc ccggccaggt
     7561 gcccgaagag aaatgttgga aacctatatg cagagattat ccaaactgga agaaatagcc
     7621 aatagtatag atggagtaag taaatcgtat gccattcagg caggcaggga attacggata
     7681 attgtagagc cctctttgat tgatgaaaat caaactgctt tattggcaac cgaaattgcc
     7741 gcttctattg agaaacaggt tcagtatccc ggccaaatta aagttacggt tatcagagaa
     7801 acacgccaga tagcaatggc aaagtaatga aagttctatt ctttggtgat gtttacggta
     7861 aagcaggtag gcaaacagtt cagcttactc ttccggaatt gaaagaagaa tttcaaccgg
     7921 attttatttt agctaattgt gaaaaccttg cagatgggaa aggagttacc gaaaaaacac
     7981 tcaaccagct ttttaatgca ggagttgatg ctgttacagg gggcaatcat ttatgggata
     8041 gaacagaatc tctggaatat attcgcggtc aaaacagaat aattaaaccg cttaattatc
     8101 ctgaagatgc tccgggaaat ccttttatta ttttagctaa ggaaaacctg cgtttgggag
     8161 ttctctgtct ttgtggtcaa ttgttgatgc caccgtgtaa taatcctttt acagcattgt
     8221 ctgagtttat aaagttacat tttacaaaag acgctgaaca ggtctccctt tttcctttgc
     8281 cctatcctgt tattttagat tttcattgtg aagcaaccag cgaaaaaaga gctatgggct
     8341 ggtttgcgga tggacagctt agtgctgttt taggtactca tactcatatt cagaccgccg
     8401 atgaagaaat tttgcctttg ggaacagctt atattacaga tataggtatg accggttcgc
     8461 ataatagtgt aatcggagtg aagaaagaaa ttattattga aaaattccgc acttctgttc
     8521 ctaaccgttt tgagtcctcc gactcaggtt tacaggttaa tgctgtttgt ctggaattgg
     8581 attccgaaac agggaaagca ttaaatatta tgcgtataag aagaaatatt gaataaaatt
     8641 atggagaaag ttttaaacgg aaaacccgtt gcccaattga taaaccggac ttgttccagt
     8701 ttaatagcta attatggatt gaaaccgcaa atggttttaa tccaggtagg ggatgatcct
     8761 gcttccactt actatgtcca aagcattatc aatagcggaa agaaattggg ttgcttttgt
     8821 caaatgaaaa tcctgccgga taacataagc gaacaggaat tattagcgat cctgaacgaa
     8881 gctaatcaca atcccgatgt taatggcata atgttacaaa aaccgcttcc taaacatata
     8941 aacgaaagca aagttaattc agctatcaat ccggaaaagg atttggactg tctgcatcct
     9001 ctaaatttgg gaaaaataat gatggaagaa gagggttttc ttccctgtac tcctgctgca
     9061 gttctttttt gtatgctata ttatggaatt gaaacccaag gtaagcatat agtaatttta
     9121 ggtcgcagta atgtagttgg caagccctta gctaatattc tgctctggaa aagaaatttt
     9181 gcgaatgcta cggttactgt ttgccatagc aaaacaaata accttgctga gtttaccaag
     9241 gaggcagata tcctcattac ggcaataggc aaagcggaat ttgtaactcc tgaaatggta
     9301 aaagaaaatg ccattatttt ggatgtggga ataaatgaaa ttaaattagc aaacggagaa
     9361 agcaattatg tgggtgatgt taattataaa ttatgttacg ataaggcatt ggctataaca
     9421 cctgttcccg gaggaatagg cagaattact acctcagtgc tctttttgaa tttggttgat
     9481 gcctgtctta aatccagaaa tacaaacaaa agtattgacg aatatatcca tcttattttt
     9541 gatggtaaat aatgaaattt actaattccg atgtttggga ggaatgatgc agttgaagag
     9601 tgctaaactc ttattcatca tagtgatgat tataagtagt gtattgctga tttcgggctg
     9661 cggaaaatcc ggaaaccgtt ttgccaatca gccaccaaca attcaaataa cctcttttga
     9721 gggctgggat acaacttatg ttagtgccgg ttacgataca actgaggttt atagttttca
     9781 gcagcgtatt tattggcatg ctactgatcc tgatggagta attaccggct atgcattcag
     9841 aattttggat gaaaataata atcctgttcc tactcccggt tatgaatata tagatttaac
     9901 cggagagctt acccctgata atcttttagc tttgggaacg ggatgggtaa ttcactatat
     9961 gactggagcg gatcaaaata taccgcttga tgatccacaa gcacgtcgtt ctatttggac
    10021 ttcgcaaaaa tatgccgtaa tcaattttcc ctcagctgat tctctcggga atccgattgt
    10081 taaatttagc agatttgaag tggtagctat tgataacaga ggtgctatta ctcctcatcc
    10141 tgcctggagg aatttccgta ctcaatcgga tcgtccaaaa tgtatgattt cttccactaa
    10201 aggtaacccg aatggcaaag atgtaggttc cggattaaaa cttgccttct caatgagcga
    10261 taccgatcct tttattactg cggttcccta taaatatgaa tttaaaataa tgaaaataaa
    10321 taaccaggga gaagtgatat caggaactga atcggattgg ttttccacta ctggtgaaga
    10381 taatagaact aatagaatta atgagtatct gcttacaaaa tatacaaacc cgcctttatc
    10441 ttatgactat gatgaagtca cattggcatt caaaaaccaa agaaccaaaa ttatatctcg
    10501 ggttacagat ttggcaggag ttatatctat tccggatgct gcatctacga tgattttctc
    10561 tgtccgtcct ggatttagac ctcatacttt tatctatcct acaaaaacct atgctctaac
    10621 caacaatcat tttgaagatt atggtgatga taccaccgaa gaaataaaag ttatacccag
    10681 agtagttacc gagggagtaa cgcgttatgc tcttcctctc tttaaggatt ttgagggtaa
    10741 atatactgct gtctattcta caaatattaa actttggatc cgttggggct ggtggggcga
    10801 atatggagaa acaagagggg aaactgttac ttatggtgat acaattaaaa tcaaaacaac
    10861 tccttacaat aaaaaagtgg atgtagtttt ggatagccaa acagggcaga attactttag
    10921 cgaaataact gcctttgatc ttcgtttaga tggtgaaccc tataactatc ctccctatcc
    10981 ttttagtaaa tatggcttta ccgatcaaaa cgggaaaaaa tggttgcgcc ttcctgtaaa
    11041 ttctcctttg ggacaaacag tcgttcttac agctggtcag ttacctcttc ctccagcaac
    11101 agaaccggga gaacacaaat ttgaagttag agtagtagat ctacaagatg aatatgatcc
    11161 taccccagtg gattttactt tctatttaca tcgttacata gaacctgcta accggcaggg
    11221 agttctgata attgatgatg atccggttag tgctcaagta aacgatgcac taattactca
    11281 aaggtatcaa gctatgcttg aaggatatag cggaaatgta aatgtaataa ccagaacaga
    11341 aaataatgaa gatattcgtc aaagagccat tgcttttagc gacctccaaa aatataaatt
    11401 agttatctat catactgaca attatgagaa gacgggtaat ttgcaacttg actttgatgc
    11461 ctatagttta ttcttaatga gaggcgggaa tctgttaatt tcacacacta gtcttttagg
    11521 agcacaatta acggaaattg ctaatggagg tttacgcaaa acctttgtta ccaatctggg
    11581 atttaataaa attcccaaag tatcttacct aaataattca aatagtcctt tctttcaaaa
    11641 agctgtttct aatatgacag attataacga tttaaatttg cattatgatg ttacgggttc
    11701 acctgccatt catccgctta ttgatttacg tgatggttta ggttatttat cctattttga
    11761 gaatggtaat ttttcgggtg atattcttta taaattcggt tgtaagccta caacctatcc
    11821 tacttatcca cccaccagcg agcaatttga taaatataat ggtaaaacag ttgctttccg
    11881 cagaacaact tcttccaacg ggaaggttta tgtctttggc tttcctttat cctttatgaa
    11941 agttgaagat acgcgtccga tgatgaataa aattatttcc gaactgatgt aactttcatt
    12001 cttggaggaa aaagaacttt gtctttttga gagttcaagg ttcaaagttg taacattctc
    12061 tcttgaaggg aaaaagaact ttgtcattcc tgataaagca ggaatacaca taaggttagc
    12121 aaggtattta tgtcttttca acataaatac agtaacatat cctgctgatc cagtcatctg
    12181 tgttctgttt ttgggatcgt cacaagtcct catcgctaac ttaatttgtg acaatgtata
    12241 aagttatata agcgatgacg actcctgact taattgaaat tagtgattgt gtcttctttt
    12301 taacgagggg cttacgccac cttctacatt attgtatcac ccttcgggct ttcaaggaat
    12361 taacaaatct caaccctctc aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnttttatc tttgtgttcc aattccgagt atgttatctg ccattgtgtt gtatcatgat
    12541 ttaaaataag tgttccatta tgttctttta atcggttgta aattagtttt ttagcggtaa
    12601 ctgtctggat gcgggaaaca tcataagcgg aagtgattgc cagggaagta tgaaacaagg
    12661 taggtataat atctcctgca tagatataat agtcagtgcc gaaatcaatt gtaacaattt
    12721 gactgccaat agtatgtccg ccaacaagag taagcaagat tccgggttca atttctgctt
    12781 caccatcaat tatagttata tttccctgtt tttccagcaa agccaattgc tgttcaaagt
    12841 tgtaagctga gcgatttagt tcatcaggat tttttgctat ttcccattcc gctttttgaa
    12901 tccaatagtg tgctttagga aaagtgaggg catcataagt tccaaagtta gtaatgattc
    12961 cacctgcatg gtcaaaatgc agatgagtca taatcacatc ggtaatgtct ctatccttaa
    13021 gtcccaattc cgcaagagag aaaggcagga gaaattcgct cggctggtaa atatctcttt
    13081 gtttatcgga aagtcggttt ccgattcctg tatctattaa aatattacgc ttgccacttt
    13141 gaataagaag caggtttaaa ttcagtcggc gtctttgtct ctgatccaga atggtctttg
    13201 tttgccataa aggataggga agaacaccca taaaagcacc gcaatcagac caatagtaac
    13261 cagccgagac agggaaaatt tgtttcattg atgtttggtg ttgcttactt ttggttgttt
    13321 ttgttggcga ttgggttttt tcacttttaa ggaggtattt ccggttttat tgtttggtgt
    13381 gggaacgggt ttatgagtat ggattggggc tttgttgttt ctccttttgg gttctgttct
    13441 gctttgggaa agtgcttctt tttgattttg ttcggcagaa aaaacaacta tgcgtttctt
    13501 ttctccttca ccgatagtga gggtgcgtaa gcccttttcc tgttccacat agcgatgaat
    13561 aattcgtctt tcagcagcag gcattggttc cagagttata ggtttgcctg ttattttaac
    13621 ttgcagaatt tgaggaagat agggctttaa aaaactgtct gcctgacgtt ccctataacc
    13681 atcaacatcc aaataaattc tctcaatttg agcattgtct tcaaacacac gattgattaa
    13741 atattgcaaa gtatccagca tagaaccatt tttcccaatt aaaaatccgg aatcgggact
    13801 accgttgatt tccaaaaaga gggttttgcc ttcggttctg ctttttaaag aagtgtagct
    13861 aattcccatt ttttgcaata agttctgcat aaaaagttca gctctgtcaa aagatgaagg
    13921 cagttgaaaa cgcaccagtg caagtttatg ggcaaataag ccaaatatac ctttagtggg
    13981 ctttttcagg atttcgtaat ttagttccca atcgtgaatt ttatagtcct cgcggaaagt
    14041 ccttattatt tcttccaggg attctccgct tttatctata gtagtcattt attttccttt
    14101 ttcttgaggt gtttggttaa aaaatactga tgaagaatag agagcacatt aaaagctgtc
    14161 caatataaaa caagtcctgc tggcatcccg cgaaagataa tcaccataaa aataggcatt
    14221 ccccaagtca ttattttcat ttgggactgc tgcatttttt gttgttccgt catttgcgag
    14281 gtatccacat ttttaggcct catcattaac gactgtagta gcataaaacc gcccataata
    14341 atcggcagaa tcatataagg atcgggttca gaaaggtctt tcagccataa acaaaaggag
    14401 gcattacgca tatccaaaga atagcgtaaa acgctgtaaa ggggaataat aatgggtatt
    14461 tgaatcagca aaggcaggca gccggcagtc atactggttt tgttttcctt atagagtttc
    14521 gtaagctctt cccgttgttt cactacatca ttagcatatt tttttctgat ttcctctata
    14581 tagggttgca ttttttgctg acgcatactg tgcaccattt gatgttgcgt taaaggatgc
    14641 agcaaaagct ttataataac ggcaaaaata agaattacca ccccataatt tttaatgtag
    14701 cgatgcagga atttaagaat ccattcaaaa atattggcga gccaacgtag ccagcttgct
    14761 cctctttcag gaatgttatc catctgtttt ccatatttct gcagaatttg atagtccgtt
    14821 ggtcccgcat aaattataaa actttgactc caactttgtt tgggattgct ttgtttagaa
    14881 tcaataacaa acgccggatt accggtttga ggattcaatt ctgttctaaa ggaacgcatt
    14941 aaagcgggtt cattttcttt taggacgaga gtaaaatatt tacctctcaa agccatccaa
    15001 ttgaaactgt taagatttcc agatggctgt tttttcttca ttttggcgag agttgtctta
    15061 aaaatttcgt tatcggcata atacataaag cgataatcct gctgtttgct tttggttaca
    15121 gtttccgtat ccgcaatacc acaggaaaaa tcaacttcaa taccattaat aacttgataa
    15181 ttttccacat ttatagcgaa agatatgcca tattgattat ccaaggcaaa gcttttacta
    15241 atttggggat ttgtttcatc acccaggaaa aaagttactc ctaaagaatc gggagaaatt
    15301 ttatattgaa aaataacatc ttttaacggt gtttcaccta cgggatgaat caattttaca
    15361 tctgccagag aactattttc gggaattagt ttcacttttg tgctgtcgtg cattataaaa
    15421 ttatgcaatt ccacttgttg aatagaacct ccgcggctgt tgaaggtaac cgtcatcaac
    15481 tgatttgaga gttttattat ttccggtgtt ttttcctttg ccaaaagagt atccggcatt
    15541 ggagtaatga taatggtatc cggttttacc ggttcagtaa ctgtttgagg agtttgggtt
    15601 tgttgatttt gctgttcctg ctgttgacgc aattgttgag gtttccaaac aaattgattg
    15661 aaaacaagga aaaggatgaa caccaggagt aaagcaagta aggttcgttt ttccatttat
    15721 tgtcctttag ggaagtgggt cgtaaccacc cttgtgaaaa ggattacaac gcaaaatacg
    15781 ccaggcggtt aaataaaatg ctttaaagaa attgtatttt ttaaatgctt cataactgta
    15841 aacactacag cttggttcaa atctgcaact ggctggtaaa aaaggagata cggcaagctg
    15901 ataaaaccga atgagggcta aaaaaagcag attcggaaaa tgtaaaatct gtctcaagac
    15961 cttgtttgta tgttcagctg tgctattatg tttgttaatt ctcgggataa ttcctgccat
    16021 tctaattcag tagctccact tctggctatc agattaaacc ggtaaccaac aggtattttg
    16081 tcctgattgg tgcgcaacca ggatttgatt ctgcgtttaa gtaaatttct tttaacggct
    16141 ttgcccacct tattgctgat agtgatgccc actgcaattt caccttctct gtataatcca
    16201 ggcacataaa aataggaaga cctgatcata aaatccggct taaaaaaatc agaatattca
    16261 ttgtgtgaag taatccattg caacataagg ttatactgtt aatgtctttc ttccttttgc
    16321 tcttctccga gctaaaactt tgcgtccatt ttttgttgcc atccgactgc gaaaaccgtg
    16381 agtatttttg cgtgatctgt ttgagggctg gtaagtgcgt ttcatttttt ctctatctcc
    16441 ttgaatatat atgcaattat gtaagtaaag tcacaaaatt tgttctgcct gctatgtcaa
    16501 ggatttttgt tttttacacc gaaaac
//
LOCUS       DAOOZV010000041        15873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3763_length_15873_cov_66.197244, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000041 DAOOZV010000000
VERSION     DAOOZV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15873
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_15873_cov_66.197244"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /gene="thyX"
                     /locus_tag="PLW08_05370"
     CDS             complement(<1..1367)
                     /gene="thyX"
                     /locus_tag="PLW08_05370"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPL52069.1"
                     /translation="MKVNIAGFNIDKELIDKLNSPLATPELISASYARISRSNKSIEE
                     LRKEAMKELDKARKTNTNIIFAMGHSSVAEHSVFNIDLIGISRALTELIQRSRIASFT
                     EKSQRYVTFSKDYVVPAEFANRPKLKQPYKKLMDELFAEYELSFNALNELYKKQKPEL
                     KTRERECLAKEDARYILPLSTKTQMGMTINARSLENLLRRLSKSPLKEAAELKELLYT
                     KVSAIAPSLIRYIDNDDYTGKVDLEKVGFTGFLQQELPWVKELDLENKVRVINLPTNA
                     DDTILASIIYQQGEFNWNETKETVAHLPRIIKQQLWNQVFKDLKPWHKVPRAFETVDF
                     SFELSMSESCWAQFKRHRYCTALRKAGSSLTAKIPPAIKAVNRENIWKELNNKIIRMG
                     SVLPPKLAYLNPYFRTNASIVTVFVKMNLREIYHFVRLRSDENAQWEIRELSQLMAAQ
                     VKKNAP"
     gene            complement(1380..2156)
                     /gene="deoC"
                     /locus_tag="PLW08_05375"
     CDS             complement(1380..2156)
                     /gene="deoC"
                     /locus_tag="PLW08_05375"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPL52070.1"
                     /translation="MKDIPQIAKELFGDNHPAQCNGGHIICLNCYHCHLKFPDQIYDP
                     NQGLASVIDATILKAETKKEEITELCKTANQYKTASVCVNSHFIPLAKGLLEKPVKSC
                     TVINFPLGASCPQSIVNEAKAVLEIGAEEVDLVQNLSAFLSGDYDNDLQCMQEVVKLC
                     KKHKALLKVILETCYLTEEQIIISCLLAKKAGADFVKTSTGFGTAGATKENVALMRKV
                     VGPKIGVKAAGGIRTKEQALAMLSAGANRIGASSVKTILQ"
     gene            complement(2153..3205)
                     /gene="rlmN"
                     /locus_tag="PLW08_05380"
     CDS             complement(2153..3205)
                     /gene="rlmN"
                     /locus_tag="PLW08_05380"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPL52071.1"
                     /translation="MLTNIFSLMPEDLAKSIISRQPDLPEYRIKQVLSWLYKFYLNEP
                     EKMTNLPEEFRAFLKTNYSFFLPEIESKLVSQDGAVKYRLLLEDGEIIESVLIPTEKK
                     NTLCLSTQVGCARNCKFCATGKMGLIRNLETQEIIGQVIIASKELKNSGTAKLTNLVL
                     MGMGEPMDNLKNVLMALQILQSNAGFSFSPRRITVSTCGVVPGIIALADSGIKAKLAL
                     SLISAVEAKRKQLMPVNNQYNLIQLKQALLYYLRKTSFRITIEYILIPNFNMDSEDLA
                     ALRKFTGDLSCKINFIPYNPGRNSPFRAPTETEIKDFMQRAQKLPQAITLRKSRGADI
                     FGACGQLTKNNLGEKK"
     gene            complement(3199..4038)
                     /gene="ricT"
                     /locus_tag="PLW08_05385"
     CDS             complement(3199..4038)
                     /gene="ricT"
                     /locus_tag="PLW08_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPL52072.1"
                     /translation="MSDFIEVIFRTGRIGYYQNTKGLNIQPEDIIIVEVERGDDIAQV
                     IHLGITEEELDAQVLTGKTLSIRRQANSEDLEKMKNLSYEEDKAAKTFLGVLDHYPFE
                     MKLIETIYQFDGNKLTFFFTADGRIDFRNFVRELANIFKTRIELHQTTGRDEARRLGG
                     FGMCGKQYCCGSFLKRFNQVTIKMAKDQNLAGNLTKISGPCGRLLCCLNFEEDFYVEE
                     AKGFPLVGTFVTYQNTKMMVFRLNVLAKKVMLASEEGIIQEIDLDEFSRLPVISVPIL
                     EEC"
     gene            complement(4038..5231)
                     /locus_tag="PLW08_05390"
     CDS             complement(4038..5231)
                     /locus_tag="PLW08_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HPL52073.1"
                     /translation="MQTVIFDDDALSNFYPLTLNRSIGDLRCGITKLRQRLESIFSDS
                     EETCVLIDESLVPLYQERHPDWIINSAVRGEKLYLNSRIIITADAVEQIQKLDFGSCL
                     YTGQNIVAIKVKDTSIFPDVAKLKKKLCNIKLYNCLADIIQDNSRMLMRDYHLHFYDG
                     ENYFETEPGVTVLHPYNVWVGEGAEFSPGVILDASEGPVVIDTGAYIMANAVLCGPVY
                     LGKKSLVKIGAKIYGGTSIGPVCKVGGEVEGTIFQGYSNKQHDGFLGHSYIGEWVNIG
                     ADTNNSDLKNTYKTVAYYSYTHKAKIDSGNQFLGCIIGDHSKTGINCSINTGTVIGIG
                     CNLYGPNLIRDFIPDFSWGEAEKLEKYRFPEFCETAEIVKQRRNLSFTATEKELYKNI
                     YNMRI"
     gene            complement(5212..6381)
                     /locus_tag="PLW08_05395"
     CDS             complement(5212..6381)
                     /locus_tag="PLW08_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL52074.1"
                     /translation="MYRILFYILCVLVSTGCITYLVLCIRFVYGFKHYKPVFSFKKRT
                     VSVVIAARNEAKNLQDLLLCLLNQDYPSELFEVILADDDSEDETAEVANKYIEAGLNL
                     HYLKITGRENVLSPKKKALEDAVYTAQGEIILSTDADCIVPTTWISSMVSLFTDDVSM
                     VAGYSRTLLPDWKKASLVQKYEHLDYALSYIALAGCYTIGKSWACVGQNLAYRRSAFY
                     DVGGFSKIKHLLSGDDVNLLQLMRNKGYKIIFNFSPSSFVYTQPIQSWKQLINKRSRW
                     TSNMKYQLKFNPEFYFILFSLACLYWGGLIMLFFNYKLALFILAYRIIIENIIFNSSR
                     SRFGITKEIMKFYPVWLIIQSFFLVFTMILGQFNIFVWHGKRPNNKRNNNANRNL"
     gene            complement(6400..8448)
                     /locus_tag="PLW08_05400"
     CDS             complement(6400..8448)
                     /locus_tag="PLW08_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPL52075.1"
                     /translation="MKASTKEKLYSVLKYLWFAFCFITGIFVGALWFYHDSLPPTSEL
                     RNYTLRSGSEVYDRNGKMVYLFAFEKRKLVSLKELPQYLIDALIITEDKHFYHHFGID
                     ILGNIRALAVDIARLDFSQGASTITQQMARNMFLTLDKRLSRKLKEIILALRIEREFT
                     KDEILEIYFNKIFWGGQIHGVETASLYYFGKHARDLTLAESAALAGMIQRPNYYNPAK
                     HPDRLIERRNMILGKMLKAKKITQEEYNEAVASKITGDRSSLQQYSTDYYIEHIRLYL
                     EHKYGTEKLFEGGLKIYTPMDVDLSVYADSVLNRYLSNVEKGYPASMRYDSVPKNSVD
                     INTRYLQGGILLMENSTGQVLAMLGGRNFTHSKFNRITQAKRQPGSAIKPTYYTAAIE
                     KGYTPATVINDAPITLTGGNGKDWTPQNFSRKYYGPTRMRTAITYSYNVWAVKTVMDL
                     GLDVVNDAFRRFGIDAKAEDYSAALGAYEVTPINLISAFTTFPNNGFRTKPVFITKVE
                     DVNGKVLERSSTAKYDVTTPQVAFIMTSLLQSVVTSGTGAAARNNYYWQAAGKTGTSS
                     DHRDAWFIGFNRKYTLGIWNGFDNNAAISASASCAPIWGQIMTKAISNENKGRLPKSD
                     DPRYSFVVPDKIVKRTINPKTGFLSENGIEEYFIEDNIPPARSDTLSYNYYPTRWGNP
                     "
     gene            complement(8445..8969)
                     /locus_tag="PLW08_05405"
     CDS             complement(8445..8969)
                     /locus_tag="PLW08_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPL52076.1"
                     /translation="MNIHNKSKLTQLAFYTALAASIHIVEDLIMRMLPLPFLRVGFSN
                     IVILYLICHNRIGEAFIVNISKTILSGIVTLTLLSPSTLLSLTAGISAIIVMFLVRCL
                     HLGLGIYGISICGAIAHNLTQLAMVRAILIHSDKVFVLTPILLSIALISGSIIAYLTL
                     YIDRKSEKAKITER"
     gene            complement(8966..9682)
                     /locus_tag="PLW08_05410"
     CDS             complement(8966..9682)
                     /locus_tag="PLW08_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL52077.1"
                     /translation="MIEVEKLCVSLNDREILVDISFTLNNGNNMVILGRSGSGKTVLI
                     KTLLGIYFPVSGSVVIDGIDIHRSSEDQRKTTKKRFAMVFQNAALLDSFTVLQNVALP
                     LYERGEKDTALIQDRVHKCLQMVGLENTVNLYPAQLSGGMRKRIGIARALVYEPDYII
                     FDEPVSGLDPITSREIMYYITRIVETASATLITITHELKDIRTIANQVLFLNEGRVIF
                     NGKTSDLYQSPNPLVQEYLQ"
     gene            complement(9669..10025)
                     /locus_tag="PLW08_05415"
     CDS             complement(9669..10025)
                     /locus_tag="PLW08_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPL52078.1"
                     /translation="MKFFRTLYPTLIWFCLIWILSSLPSQHIPSVNIIGFDKLEHIGI
                     YAILGCLLGYWLIFKDWKLTTVILIYVFLLLLAGLDEYHQNFIPGRDVSLYDFMANSA
                     GIIIGFLFYLHKYDRS"
     gene            complement(10022..10687)
                     /locus_tag="PLW08_05420"
     CDS             complement(10022..10687)
                     /locus_tag="PLW08_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPL52079.1"
                     /translation="MLIASTAEYYMKALGIKLAEISIQISENEPVIEIKAKSLKETAI
                     SPQLNNIYRIEHNGSYQPITYTRVIRQKNVNDKVIVNYNHNSSQATMIRLSDNSTIQY
                     SIAKNSQDVYSFLAEVIAGRVQTGKYPIDANGVKWQANVVQLASETVDTPLGKYPARH
                     YEITFHNLTEKKMPYIDMVTFNMLQENNKLNLWVYNNQYAVKATFKKKGLSSCWELVD
                     IKK"
     gene            complement(10723..11988)
                     /locus_tag="PLW08_05425"
     CDS             complement(10723..11988)
                     /locus_tag="PLW08_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPL52080.1"
                     /translation="MDDGKAFIIIVLFALSAFFSGSETALFSLSRIQLKRLENNDKSS
                     AKRIIKLLSKPRQLLITLLLGNTFVNLAISSFGTLLALQIGTKYGYTESSVVTVQIII
                     TTILILFLGEMTPKLIALSKADSFSKIISLPLMGFRLIFFPVVWVLEKISELISKRKA
                     VDQYIGTQFTHEEFHNLLQSESSSHSLEEHEKKMLVGLFRFKEAEIKEIYVPRVKITA
                     IEENKDLEELKQIIIDSGYSRIPVYRGTIDEIVGIIYVKDLLLYPEKKTIKELMRPVW
                     FVTENMKVQALLNQFKQKKLQIAVVVDEYGGTSGIISLEDILEEIVGDIRDEYDKDEI
                     PELIKKDENTYIISGNFSVRQFNEDFSTEISVEDYDNIAEFLLAYFNNVPAIGATAKY
                     DDKLEFTILDADEKSIKQIQVKIKPGELS"
     gene            complement(11991..12455)
                     /gene="ybeY"
                     /locus_tag="PLW08_05430"
     CDS             complement(11991..12455)
                     /gene="ybeY"
                     /locus_tag="PLW08_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPL52081.1"
                     /translation="MNSLELIGETIIPESEQEKIAELICSREDPEHSYQICVKTVDSE
                     TMKHYNKRYRGLNETTDILSFITADLPACAIEERDEEDGFSAKPVRICDIIIDIKRLF
                     QQKGIRTLEEEYRAVLIHGLLHLVGYDHIRSADAEKMNKKEEYYLKQTQGEI"
     gene            complement(12448..14544)
                     /locus_tag="PLW08_05435"
     CDS             complement(12448..14544)
                     /locus_tag="PLW08_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPL52082.1"
                     /translation="MNSKYILYCIVTALIIVGIYQIVAVGRVNYPEYNLKEGQVSEVE
                     IIAPFDFPVLKSPQELAAEKEQVLSQVDKPYEISEEPLFEAISNLDNIYAVLCSIEPT
                     DDWEKIASDLKRAGYSFSPEALEFTLRSSLRDRIYESLRRELGTLYNQGIYESLEEDS
                     ILIWQNDNPEKKALSDFLPREKAKEILIDKFPEAKTFVSEFTEQYLKPNLLVNEEKLN
                     ELNQSSLQTVQPSEGMVLKNEVIVRKNSRVTEEEVRKLESLQEAYKRQKIRKSPLQEM
                     FLSLGLLMFIFVVIFLANHYYGVQSKEEKIPVADFLPVNLGFIVLVILTVLSNFVLSG
                     SNLIIPFAMIAIAGAILVNFEFGLLYSVCNILVISPFLNWETFTPVIMLLSTIMCLLI
                     LRRQKVWHEYLMIWLYLLISLLVVIICLGIYKRDSIITIMRSIGYAFISASLSITGIL
                     LIVPYYEKKWNRATKQTLLELLDFNHPLLKRLATEAVGTYHHSLVVGNLAEHAAEAIG
                     ANPLLARVGSYYHDIGKIINPEIFTENNEDSSEIHSQIEPNESAQLIRNHVTEGITLA
                     KKYKIPQPVIDIIMQHHGDMIIRYFYEKANKEGKELDTNAYRYPGPRPQTKEAALVML
                     ADIVESTTKAKEIESETDIAKIIDETITYLLKEKQFDEAPISMKDLKIVKQSFIPVLE
                     SIYRKRLDYPEAQKDE"
     gene            15258..15731
                     /locus_tag="PLW08_05440"
     CDS             15258..15731
                     /locus_tag="PLW08_05440"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPL52083.1"
                     /translation="MIQLSELPVSYGKIHLPAKVALVVTQKPESGYNLITIEWFMRTS
                     LQPPMFAISIGKTRYSHECLEANRYFNLVFPSLKMKPLLTLCGSNSGRDMDKFAVGKV
                     EYIAGKLHKLPVLKDAVACFECEIVSQIRSGDHTIYIGEVHYCWQNPEEELFYYQ"
BASE COUNT         4828 a         3158 c         2784 g         5103 t
ORIGIN      
        1 ggagcatttt ttttaacttg tgcagccatt aattgtgaca gctctctaat ttcccattgg
       61 gcattttcgt cactcctcaa ccgcacaaaa tgatatattt cccgtaagtt cattttcaca
      121 aaaacggtaa caatagaagc attagttcta aaataagggt ttagatatgc taattttggg
      181 ggaagtacac tgcccattct aataatttta ttattcagtt ctttccatat attttccctg
      241 ttaacagctt tgatggcagg aggaattttt gctgttaaag aacttcccgc tttacgcaaa
      301 gcagtacaat agcgatgtcg tttaaattga gcccagcaac tttcgctcat agaaagttca
      361 aaactgaaat caactgtctc aaaggcacgg ggaactttat gccagggttt caggtcttta
      421 aaaacctgat tccagagttg ctgtttaatg attctgggca aatgggcaac cgtttccttt
      481 gtttcattcc aattaaattc tccctgctgg tagataatac ttgctaaaat agtgtcatca
      541 gcattagtag gaaggttaat aaccctcact ttattttcca aatccagttc tttcacccaa
      601 ggtaattcct gctgcagaaa accagtaaaa ccaacctttt ccaagtccac tttacctgtg
      661 taatcatcat tatctatata acgaattaaa gatggggcaa ttgccgaaac ctttgtatat
      721 aataattcct tcagttcagc cgcttctttt aagggacttt tacttaaacg cctcaagaga
      781 ttttccagag aacgggcatt aattgtcatt cccatttggg tttttgtgga aagaggcaaa
      841 atgtaacgcg catcttcttt ggcaaggcat tccctttccc tggttttaag ttccggtttc
      901 tgttttttat acagttcatt taaggcgtta aaacttaatt catattcggc aaacagttcg
      961 tccatcagtt tcttgtaggg ctgtttcagt ttgggacgat tcgcaaattc tgcagggaca
     1021 acataatcct tgctaaaagt tacatagcgt tgagattttt ccgtgaagga agcaattcta
     1081 ctgcgctgaa tcaattctgt taacgccctg gaaattccga ttagatcaat attgaatacc
     1141 gaatgttctg ccacagagct atgacccatt gcaaagataa tattggtatt ggtttttctt
     1201 gccttgtcca gttctttcat tgcctctttt cttaattctt ctatgctttt attgcttctg
     1261 ctgatacgag cataagaggc ggaaatcaat tcaggagtag ccaaaggact attcagttta
     1321 tcaataagtt ctttatcaat attgaatccg gcaatattta ctttcatttt gtgtaatccc
     1381 tattgtaaaa ttgtcttcac actggaagct ccaattctgt tggcacctgc agaaagcatt
     1441 gccagtgctt gttcttttgt tctaattccc cctgctgctt tcactccgat tttaggtcct
     1501 acaactttac gcattaatgc gacattttcc ttagtagcac ctgcagttcc aaaaccggtg
     1561 gaggttttca caaaatccgc tcctgctttt tttgccagca agcaactgat aattatttgt
     1621 tcttctgtaa gatagcaggt ctccagaata actttcagca acgccttatg cttcttacat
     1681 aatttaacca cttcttgcat acattgcaaa tcgttatcgt aatcaccact taaaaaagca
     1741 cttaagttct gaaccaaatc cacttcttcc gctccgattt ccaaaaccgc ttttgcctca
     1801 ttaacaattg attggggaca agatgctccc aagggaaaat tgataacggt gcaactttta
     1861 accggttttt ccagaagtcc tttcgctaaa gggataaagt gtgaattaac acaaacggat
     1921 gcagttttgt attgatttgc cgttttacag agttctgtaa tctcttcttt tttggtttct
     1981 gcctttagaa tcgtagcatc tatcacggaa gccaaaccct gattgggatc ataaatttga
     2041 tcggggaatt ttaaatgaca atgataacag ttcagacaaa tgatatgtcc accgttacat
     2101 tgagcgggat gattgtcgcc aaaaagttct ttggcaattt gaggaatatc tttcatttct
     2161 tttctcccag attatttttt gtaagttgtc cgcaggcacc aaaaatatct gctcctctgc
     2221 ttttgcgtaa agttattgcc tggggcaatt tttgcgctct ctgcataaag tccttaattt
     2281 ctgtttctgt aggagctcta aaaggggaat tcctgcctgg attgtacggg ataaaattta
     2341 ttttacagga taggtcgccg gtgaattttc ttaaagcggc aaggtcttca ctatccatat
     2401 taaagttcgg aatgagtata tattcaatag ttatgcgaaa ggaagttttt cttaggtaat
     2461 ataataatgc ctgtttcagc tgaataaggt tatattgatt gtttacaggc attaattgct
     2521 tgcgttttgc ctcaacagca gaaattaaag aaagagcaag tttggcttta attccgctat
     2581 ctgcaagggc tataataccg ggaacaactc cgcaggtaga aaccgttatg cgtcttggac
     2641 tgaaagaaaa ccctgcattg ctttgcaaaa tttggagtgc cataagaaca tttttcaggt
     2701 tatccattgg ttcacccatt cccattaaaa cgagatttgt aagctttgcc gtgccgctat
     2761 tttttagttc tttacttgct ataattacct gccctataat ttcctgtgtt tccaaattcc
     2821 taatcaaacc catttttcct gtagcacaaa atttgcaatt gcgggcacaa ccaacctgag
     2881 tagaaaggca aagtgtattc ttcttctctg taggaatcaa aacactttca attatttcgc
     2941 catcctctaa aagcaaacga tactttacag ctccgtcttg ggacaccagt ttgctttcta
     3001 tttcaggtaa aaagaaggaa taattggtct ttaaaaatgc cctgaattcc tcaggcagat
     3061 tagtcatttt ttccggttca ttgagataaa acttatataa ccaggaaaga acctgcttaa
     3121 ttctatattc aggtaaatcg ggttgcctgc tgataatact ttttgctagg tcttccggca
     3181 ttaaagaaaa aatattagtt agcattcttc caggatggga acgctaataa ccggaagacg
     3241 ggaaaattca tctaaatcaa tttcttgaat tataccttcc tcactggcaa gcatcacttt
     3301 tttggctaaa acattaagac ggaaaaccat cattttcgta ttctgatagg taacaaaggt
     3361 gccaaccaaa ggaaacccct tggcttcttc cacataaaaa tcctcttcaa aatttaaaca
     3421 gcaaagcaaa cgcccgcaag gtccggaaat tttggttaaa ttacctgcca gattttggtc
     3481 tttagccatt ttgatggtaa cctgattgaa gcgttttaaa aaactgccgc aacaatattg
     3541 tttcccacac attccaaaac cacctaaacg acgtgcttca tctctacccg ttgtttgatg
     3601 aagttcaatg cgggttttaa aaatattagc caattcccga acaaagtttc taaaatcaat
     3661 tctgccatcg gcagtgaaga aaaaggttaa tttattaccg tcaaactggt aaattgtttc
     3721 aatcagtttc atttcaaaag ggtaatgatc cagaacaccc agaaaggtct tagcggcttt
     3781 atcctcttca tagctgagat ttttcatttt ttccaggtct tccgaatttg cctggcgtcg
     3841 gatacttaat gtttttcctg ttaaaacctg agcatccaat tcttcttcag tgatgcccag
     3901 atgaattacc tgagctatat cgtcacctct ttccacttct actataataa tgtcttcggg
     3961 ttgaatattc aagcccttgg tattttggta atagccaatt cgtcccgttc taaatatcac
     4021 ttctataaag tccgacatta tatcctcata ttatatatat ttttatacag ttctttttcg
     4081 gttgcggtga agcttaaatt acgccgctgt ttaactattt ctgccgtttc acaaaactcc
     4141 gggaaacggt atttttctaa tttttctgct tctccccagg aaaaatcggg aataaaatca
     4201 cgaattaaat tcggtccgta aagattgcac ccaatgccga ttacagttcc ggtattgata
     4261 ctgcaattaa tacctgtttt gctgtggtct ccaattatgc aacctaaaaa ttgatttccg
     4321 gagtcaattt tcgctttatg ggtataggaa taataagcta cggtcttgta agtatttttc
     4381 aggtcactat tattggtatc ggcacctata tttacccatt caccgatata ggaatgccct
     4441 aaaaaaccgt catgttgctt attactataa ccctgaaaaa ttgttccttc aacctctcca
     4501 cccactttgc aaacgggtcc gatggaagtg cccccgtaaa ttttagcacc aatcttaacg
     4561 agagattttt tacctaaata aacaggaccg caaagaacag cattagccat tatatatgct
     4621 cccgtatcaa ttacaacagg accttccgaa gcatccaaaa ttacacccgg tgaaaattcc
     4681 gctccttcgc ccacccaaac attgtaagga tgcaaaacag taactcccgg ctcggtttca
     4741 aagtaatttt ctccatcata aaaatggaga tgataatctc gcataagcat tctggaatta
     4801 tcttgaatga tatctgcaag gcagttgtag agtttaatgt tgcagagttt tttcttcagt
     4861 ttagctacat caggaaaaat agaagtatcc ttcaccttta tagctacaat gttctgtccc
     4921 gtatataaac aggaaccgaa atcaagtttt tgtatttgtt ccacagcatc agcagtaata
     4981 ataatccggg aattcaaata aagtttttct cctcttacag cagaattgat tatccaatcg
     5041 ggatgccttt cttgatataa cggaacaaga gattcatcta taagcacaca ggtctcttcc
     5101 gaatcggaaa aaatactctc taaacgctga cgcaatttcg ttattccaca acgcaggtct
     5161 ccaatacttc tgtttaaagt taaaggataa aaattggata gtgcatcatc atcaaagatt
     5221 acggtttgca ttgttattcc ttttattgtt gggtcttttg ccgtgccaca caaaaatatt
     5281 gaactgaccc agtatcattg taaacaccag aaaaaaactc tgaataatta gccatacagg
     5341 ataaaacttc attatttctt tcgtaattcc aaaacgcgaa cgggaagaat tgaaaatgat
     5401 gttttctata ataatgcggt aagccaaaat aaacaaagct aatttataat taaaaaacag
     5461 cattatcaac ccgccccaat ataaacaggc aagcgaaaac agaataaaat agaactccgg
     5521 attgaacttc agctggtatt tcatattgga agtccaacga ctgcgtttat tgattagctg
     5581 tttccaggat tgaatcggct gagtataaac aaaactggaa ggtgagaaat tgaaaataat
     5641 tttataaccc ttattacgca ttaactgcag taaatttaca tcatccccgg aaagcaaatg
     5701 ttttatttta ctaaagcccc ctacatcata aaaagcagaa cgtctgtaag ctaaattttg
     5761 tcctacacat gcccaacttt taccaattgt ataacaaccc gctaaagcta tataggataa
     5821 agcataatcc aaatgttcat atttttgcac gagagatgcc ttcttccaat cgggaagcaa
     5881 agttctggaa taacctgcaa ccatagaaac atcatccgta aatagagaaa ccatagaaga
     5941 aatccaagta gtcggaacaa tacaatctgc atcagttgaa agaataattt ctccctgggc
     6001 agtgtaaaca gcatcttcca gagctttctt tttgggggaa aggacatttt cccgtcccgt
     6061 aattttcaaa taatgtaaat ttagacctgc ttcaatatac ttgtttgcta cctccgcggt
     6121 ttcatcttca gaatcatcat cggctaatat aacctcaaaa agctctgagg gataatcctg
     6181 attgagcaaa cacagcaata aatcctgtaa gttcttagct tcattacggg cagcaataac
     6241 cacactcact gtcctttttt taaaactgaa aacgggttta tagtgcttaa aaccatacac
     6301 gaaacggata cataaaacaa gataggttat gcaacccgtt gaaacaagaa cacagagaat
     6361 ataaaaaaga atacgataca ttcttaactt tccctcttat tagggatttc cccaacgggt
     6421 gggataataa ttatagctta aagtatcgga acgggcaggc ggaatattat cctcaataaa
     6481 gtattcttca atgccatttt cagaaaggaa acctgtttta ggattgatgg ttctcttcac
     6541 aattttatcc ggaactacaa aactatagcg gggatcatca cttttaggta ggcgtccctt
     6601 attttcgtta ctaattgcct tggtcataat ttgaccccaa ataggagcac aacttgcact
     6661 ggcagaaatg gcagcattgt tatcaaagcc gttccaaatg cctaaagtat atttacggtt
     6721 aaagccaata aaccaagcat cacgatgatc actggaagtt cctgtttttc ctgctgcctg
     6781 ccaataataa ttgtttctgg cagcagctcc cgttccggaa gtgactactg attgcagcaa
     6841 agaggtcatt ataaaagcaa cttggggagt ggtaacatca tattttgcag tactactgcg
     6901 ttccagaact tttccattta catcttccac tttggtaata aacacgggtt ttgtgcggaa
     6961 accgttattg ggaaaagttg tgaaagcgga aatcagattg ataggggtaa cttcataagc
     7021 gccaagagca gctgaataat cctctgcttt ggcatcaatt ccaaaacgcc gaaaagcatc
     7081 attaacaaca tccagtccga ggtccataac ggttttaaca gcccacacat tgtaagaata
     7141 ggtgatggca gttcgcattc ttgtaggtcc gtaatattta cggctgaaat tttgaggagt
     7201 ccaatccttt ccattgcccc ctgttaaagt tataggtgca tcgttaataa ctgtagcggg
     7261 agtatatccc ttttcaattg ctgccgtata ataagttggt ttaatagcgc ttccgggttg
     7321 tctttttgcc tgagtaatgc ggttaaattt actgtgcgtg aaatttctgc caccaagcat
     7381 cgccaaaacc tgtccggtac tattttccat taataggata ccaccttgca gataacgggt
     7441 attgatatcc acactgtttt taggaacact gtcataacgc atagaggcag gataaccttt
     7501 ttcaacattg gaaagataac gattcaaaac agaatcggca taaacggaaa ggtcaacatc
     7561 cattggagtg taaatcttta atccgccttc aaaaagtttt tccgtgccat atttgtgttc
     7621 cagatacaaa cgaatatgtt caatgtaata atcagttgaa tattgttgca gagaactgcg
     7681 gtcacctgtt attttagaag cgactgcctc attatattct tcctgggtta ttttttttgc
     7741 cttcagcatc tttcctaaaa tcatattccg tctttcaatc agacggtcgg ggtgtttcgc
     7801 ggggttataa taattgggac gttgaatcat tcccgctaat gcggctgatt ctgccaaggt
     7861 taagtcgcgt gcatgtttac cgaaatagta taaagaagct gtttccacac cgtgaatttg
     7921 tccgccccaa aagattttat tgaaataaat ctccaggatt tcatccttgg taaattcccg
     7981 ttcaattctt agtgctaaaa taatttcctt cagtttgcgt gaaaggcgtt tatctaaagt
     8041 aagaaacata ttgcgtgcca tttgttgtgt gatagtgctt gcaccttgcg aaaaatccaa
     8101 acgcgctata tctacagcca aagcacggat attacctaaa atatcaattc caaaatgatg
     8161 atagaaatgt ttgtcttccg taattattag agcatctatc agatattggg gaagttcttt
     8221 tagggaaacc agcttgcgtt tttcaaaagc aaaaagataa accattttac catttcggtc
     8281 ataaacctca gaaccggaac ggagagtata atttctcagt tcactggtgg gaggtaaact
     8341 atcgtggtaa aaccataaag cgcctacaaa aatacctgta atgaagcaaa aggcaaacca
     8401 cagatatttc aggacgctat ataatttctc tttagtgctg gctttcatct ttctgttatt
     8461 ttagcttttt cgcttttcct gtctatatat aaagttaagt aagcaattat gctaccgcta
     8521 atcaaggcaa ttgagagcag gattggtgtc aatacaaaaa ctttatcact gtgaattaaa
     8581 atggcacgaa ccatagctaa ctgtgtaaga ttatgggcaa tagctccaca aatactgatg
     8641 ccgtaaatcc caagccccaa atgcagacaa cgcactaaaa acataacaat aatagcagaa
     8701 atacctgcag ttaaggataa caaagtagaa ggactgagca aagtgagggt aacaattccg
     8761 cttaatatgg ttttactgat gttaactata aatgcttctc caattctatt gtgacaaata
     8821 agatagagaa taacgatatt ggaaaaaccg actcgcagaa aaggaagagg caacattcgc
     8881 attataaggt cttccacaat atgaatagaa gctgctaaag cggtgtaaaa agccagttga
     8941 gtgagttttg atttattgtg gatattcatt gtaaatattc ttgcacgagg ggattaggtg
     9001 attggtataa gtcacttgtt tttccgttga aaataaccct gccttcattc aaaaataaaa
     9061 cctgattagc aattgtccga atatctttca gttcatgggt tatggttatc aaagtagcgg
     9121 aggcagtttc cacaatgcgg gtgatgtaat acataatttc tcgggaagta atagggtcca
     9181 gtccagaaac aggttcatca aaaataatat aatccggctc ataaaccaaa gctctggcaa
     9241 tgccaattct tttgcgcata ccaccactaa gctgggcagg atataggttt acggtatttt
     9301 ccagtccaac catttgcagg catttatgaa ccctatcttg aattaaagcg gtatcttttt
     9361 ctccgcgttc ataaagtggc aaagcaacat tttgcagaac agtaaaggaa tcaagtaaag
     9421 cagcattttg gaaaaccatc gcaaagcgtt ttttagttgt tttcctttgg tcttccgaag
     9481 agcggtgaat atctattcca tcaataacta cactgccgga aacaggaaaa taaatgccca
     9541 gcagggtttt gattaaaaca gtttttccgc taccgctacg acccaaaata accatattat
     9601 tgccattatt aagcgtgaaa ctaatatcca ccaaaatttc tctatcattc agagaaacgc
     9661 agagcttttc aacttctatc atatttatgc aggtaaaata aaaagccgat tatgattccg
     9721 gcagagtttg ccataaaatc atatagggaa acatcgcgcc caggaataaa attctgatgg
     9781 tattcatcca aaccagcaag caacaaaaga aacacataaa ttaggatgac agttgttaat
     9841 ttccagtcct taaaaattag ccagtatcca agcaaacagc ccaaaatagc ataaatacct
     9901 atatgttcca gtttatcaaa accgataata tttacggaag gaatatgttg agaaggcaga
     9961 gatgataaaa tccagatgag gcaaaaccaa ataagagtag ggtaaagagt ccgaaagaat
    10021 ttcatttttt gatatctacc aattcccaac aggaagagag tcctttcttt ttaaaagttg
    10081 ctttcacggc gtattgattg ttataaaccc acaggttcag tttattattt tcctgcagca
    10141 tattgaaggt taccatatct atatagggca tctttttctc ggttaaatta tggaaagtaa
    10201 tttcataatg tcttgcagga tatttaccaa gcggtgtatc aactgtttca gaagcgagct
    10261 gaacaacatt tgcctgccat tttacaccgt ttgcatcaat aggatatttt cccgtttgca
    10321 ctcttccggc tataacttct gccagaaaag aatagacatc ctggctgttt ttagctatag
    10381 aatattggat agtgctatta tcagataagc gaatcatagt tgcttgagag ctattatgat
    10441 tgtagtttac aataacttta tcattaacat ttttttgacg gataacccgg gtataggtta
    10501 tgggctgata ggaaccgtta tgttcaatcc gataaatgtt gtttaattga ggggatatag
    10561 ctgtttcttt cagggatttg gcttttattt ctataaccgg ttcattttca ctaatttgga
    10621 tacttatttc agccagttta attcctaatg ctttcatata atattctgca gttgatgcaa
    10681 ttagcaaact gtgccagaat aaaaataggg ttaaaaggta tttcaggaca attctcccgg
    10741 cttgattttc acctgaattt gtttgatgct tttctcatct gcatccaaaa tggtaaattc
    10801 cagtttatca tcatatttgg cagtcgcgcc aatagcagga acattattga aataagcaag
    10861 cagaaattca gctatattat catagtcctc caccgagatt tccgtagaaa aatcctcatt
    10921 aaactgtcgg acgctgaaat ttccgcttat aatataggta ttttcatctt ttttaatcag
    10981 ttccggaatt tcatctttat catattcatc tctgatatcg ccaactattt cttccagaat
    11041 atcttccaaa gaaattatac cgcttgtgcc accatattca tcaaccacaa ctgcaatctg
    11101 taattttttc tgtttgaact gatttagcaa tgcctgcact ttcatatttt ccgttacgaa
    11161 ccatacggga cgcatcaatt ctttaatagt tttcttttcg ggatacaaga gcaggtcttt
    11221 tacataaatt attcctacaa tttcatctat agtgccgcga tacacgggaa ttcgggaata
    11281 accgctgtca ataataatct gttttagttc ttcaaggtct ttgttttctt caatagcagt
    11341 tatttttact cgtggcacat aaatttcttt tatttccgct tctttaaatc tgaaaagacc
    11401 taccagcatt ttcttttcgt gttcttccaa gctgtgactg ctgctttcgg attgcagcag
    11461 attatgaaat tcctcatggg taaattgcgt tcctatatat tggtctacgg ctttcctttt
    11521 ggatataagt tcgcttattt tttccaaaac ccatacaacc gggaaaaata ttaagcgaaa
    11581 tcccatcagt ggaagggaaa taattttact aaaactatcc gctttggaaa gagcaatcag
    11641 tttgggagtc atttctccta aaaacaaaat cagaatagta gtaattataa tttggactgt
    11701 tactaccgat gattccgtat aaccgtattt agttccgatt tgtaaagcca gcaaagttcc
    11761 gaaggaagaa attgccaagt ttacaaaagt attgcctaaa agcaaagtta tcagtaactg
    11821 tctgggtttg ctcagtaatt ttataatcct ttttgcactg cttttatcgt tattttccaa
    11881 gcgtttcagc tgaatgcgtg aaagcgaaaa aagagccgtt tccgagccgg aaaaaaaagc
    11941 tgataacgca aaaaggacaa ttattataaa tgccttaccg tcatccactg ctatatttca
    12001 ccttgtgtct gttttaaata atattcttct tttttgttca ttttttctgc atctgcactg
    12061 cggatatggt cgtaaccaac taaatgtaac agaccatgta tcaaaacagc gcgatattct
    12121 tcttctaaag ttctaattcc cttttgctga aaaagccgtt ttatgtcaat aataatatca
    12181 caaatacgca ccggtttggc ggaaaatcca tcttcctcat ccctttcctc aattgcacaa
    12241 gcgggtaaat ctgcagtaat aaaacttaaa atatcggtcg tttcatttaa accccgatag
    12301 cgtttattgt aatgtttcat cgtttcacta tctactgttt taacgcatat ctgataacta
    12361 tgttcagggt cttcccgaga gcatatcagt tctgcaattt tttcctgttc agattccgga
    12421 attattgttt cgccgattaa ttccaaacta ttcatctttt tgtgcttccg gatagtccag
    12481 tcgtttccta taaatacttt ctaaaaccgg tataaagctc tgtttcacta ttttcaggtc
    12541 tttcatagaa atcggtgctt catcaaactg tttttctttc aataaataag ttatggtttc
    12601 atcaataatt ttagcgatat ctgtttcgct ctcaatttct ttggctttag tagtggattc
    12661 cacaatatct gccagcatca ctaaagctgc ttctttggtt tgagggcggg gaccaggata
    12721 cctataggca tttgtatcca gttcttttcc ttctttattt gccttttcat aaaaatagcg
    12781 aattatcata tcgccatgat gttgcattat aatatctatc accggttgag gaattttgta
    12841 tttttttgcc aaagtaatgc cttcggtaac atgattacga atcaactgag cactttcatt
    12901 cggttcaatc tgggaatgaa tttcgctgga atcttcatta ttttccgtaa atatttcggg
    12961 attgattatt ttgcctatat cgtgataata gcttcctact ctggctaaaa gaggattagc
    13021 tccaatggct tcagcggcat gttctgccaa atttcccact accaaagaat gatgataagt
    13081 tccaactgcc tcagttgcca gtcgtttaag caggggatgg ttaaaatcca aaagttccaa
    13141 tagggtctgt tttgttgccc tgttccactt tttttcataa taaggaacaa tcaataaaat
    13201 accggttata cttaaagatg ccgaaataaa agcataacct attgaacgca ttatagttat
    13261 tatggaatct ctcttgtaaa ttcccaggca tataattaca actaacaaag aaattagtaa
    13321 atataaccaa atcatcagat attcatgcca caccttttgt cgccgtaata ttaacagaca
    13381 cattatggtt gataataaca ttattaccgg agtgaatgtt tcccaattca ggaaaggact
    13441 tattaccaat atattacaaa cactgtaaag aagtccgaat tcaaaattga ctaaaatcgc
    13501 tcccgctatg gcaatcattg caaaaggaat tatcagatta ctgccggaaa gaacaaaatt
    13561 gctgagcacc gttaaaataa ccagcacaat aaaacctaag tttacaggca aaaaatccgc
    13621 taccggtatc ttttcttctt tgctttgaac tccgtaatag tgatttgcca gaaagataac
    13681 cacgaagata aacatcagca aaccgaggga taaaaacatt tcctgcagag gcgatttgcg
    13741 gattttttga cgcttatatg cctcttgcaa ggattccagt ttcctaactt cttcttctgt
    13801 aacgcgggaa tttttgcgga caataacttc gttttttaag accattcctt cgcttggctg
    13861 aaccgtttgc agggaacttt gattcagttc attcagcttt tcttcgttca caagcagatt
    13921 gggcttgaga tactgttccg taaattcgct tacaaaggtt tttgcttcgg gaaatttatc
    13981 aatcagtatc tcctttgctt tttcccgggg aagaaaatca ctaagagctt ttttttccgg
    14041 gttgtcattt tgccaaatga ggatactatc ttcttccagc gattcataaa tcccttgatt
    14101 gtataaagtt cccagttcac gccgcagaga ttcgtaaatt ctatcccgca aagatgaacg
    14161 cagagtaaat tcaagtgctt cgggagaaaa ggaataaccg gcgcgtttta agtccgaagc
    14221 aattttttcc caatcgtcag ttggctctat tgaacataaa acagcataaa tattatccag
    14281 attgctaatt gcttcaaaaa gtggctcttc cgaaatttcg tagggcttat caacctggga
    14341 aagcacctgc tctttttctg cagccagctc ttgaggcgat ttgagaacag gaaaatcaaa
    14401 gggagcaatt atctccactt ccgaaacctg tccttctttc aaattatatt cagggtaatt
    14461 aacccgtcca acggcaacta tctggtaaat gccaacgatt attaaagccg ttactatgca
    14521 ataaagtatg tatttagaat tcataatttt aactttttta caatatagct tctctgtcaa
    14581 gtgccatttt tcctttgcgt ttatttttgc tttctgcctg ctctctcttt tatttaataa
    14641 ctatgaaaca attaggtaac gatgcagtag ctttattacg gtaatgttac ttgagtgtta
    14701 ctttggtgtt acggtagtgt tagggaatct attaggaaag gactatgaat aagtaagtta
    14761 aacgcttact tttttacctc tcccggataa cacatatttc acagataaaa aagcacacgg
    14821 acttcgtatc tttcgtagat taaacagcac acggattgcg cggattgcgt agatttttta
    14881 tttgataagc cattaacttc taatttgtga tgactttttt tgtagccgga ggatgccgtt
    14941 cctccgcaag aataaatttt tttaattagc ggagctccgg caagttccat ctacacagca
    15001 agttctgtct acacagggag ctccgtctac acaatttaat accgggggtt ttttatggct
    15061 gtcaacatac cgaaaatctg aaccttaaca accctccaaa ctttctaaat cttttttaac
    15121 ttcattttat attcgccatt ctctattttt ttctcttttc tctttttctc attgtgaaaa
    15181 tatgttcttt tttgtctttg tgagaagtca aatttggaat attatatcaa taacgacatt
    15241 tggactaagg agaaataatg attcagctct cagaactacc tgtaagttat ggtaaaatcc
    15301 atcttccggc aaaagttgca ttggttgtaa cccaaaaacc cgaaagcgga tataatttaa
    15361 ttaccattga atggtttatg agaacttctc tgcaaccgcc gatgtttgca atttcaatcg
    15421 gcaaaacaag atactctcac gaatgtttgg aagcaaacag atactttaat ttagttttcc
    15481 cttctctgaa aatgaaacct cttttaactt tatgcggttc caattcaggc agggatatgg
    15541 ataaatttgc tgtgggcaaa gtggaatata ttgcaggtaa attgcataaa cttccggttt
    15601 tgaaagatgc tgttgcctgt tttgaatgcg aaattgtttc ccaaattcgc agcggagatc
    15661 acacaattta tattggagaa gtgcattatt gctggcaaaa tcctgaggaa gaactgttct
    15721 attatcagta aggatattaa accaaaacac ttcaggaaat gaagaatata taaaataaga
    15781 aaaccaggaa aaacttggta attttaaggt ttatttcttt gcaaaagccc gaaagagcaa
    15841 aacaatgatg tagaaggtgg cgtaagcccc tcg
//
LOCUS       DAOOZV010000042        15659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_3834_length_15659_cov_56.329787, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000042 DAOOZV010000000
VERSION     DAOOZV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_15659_cov_56.329787"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PLW08_05445"
     CDS             <1..1605
                     /locus_tag="PLW08_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL52084.1"
                     /translation="AAGVQTATFSNLNSSTTYYFKIFPYTNSGTNINYKTDGTVPQIS
                     WTTASGPTIVEIILPQYIQGKNGTNNQRLPWACRLRLENLTANATYRYFGQFVVSTDD
                     ALTNGAGNCWFVNSDNTITRNTTLGMSTAGTYSEFTTNANGSYTGWFIGEPTANVRFA
                     PGNKLYYRIMLNDGAGGTSVAMRLTTANFIKVINFGTEDNVDQGTFLYGVSRSPAKNF
                     VFVYDNVEGTGRPIAGSIIENDGLDLSDITQILGSYKTNVDNVSGAWGVIIPNSVGTK
                     GFSGIKRVESRNLSDGSLYAQATDSDGVWPSGANTVNPRGGDGTPIVLNENDATLPVE
                     LSSFTANINSQGGITVMWATQSETGVNGYYINRATVNNLSEALRISSLIPASNTSQQQ
                     VYVYNDNELYEPGTYYYWLEIQDIDGVVSYYGSRSVTFGGNGNNGTPEIPLVTGIRSI
                     YPNPFNPSATIMYELDQPANVNIEIYNNRGQIVRSFNLGQQEKNRYKLLWDGTDNSGS
                     ACGTGIYFVKMQAGKETFVKKAALVK"
     assembly_gap    1673..1772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1892..3163
                     /locus_tag="PLW08_05450"
     CDS             1892..3163
                     /locus_tag="PLW08_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPL52085.1"
                     /translation="MSKIANMLDADLKGKVCLVRMDHNVVKNGIIKDTMRIDATIPTL
                     LHIYKKGGLPVLMTHIGRPFDKKTGIINISEGDSVLPVVQYLENKFQLQGLIPDCKAE
                     SKIGITDLAPIGIAIEALKKGAADFVYLPNTRWFKGEEAKDESADIFAQSLANYADVY
                     INDAFGSWQAHSSTYNIAKFLPAYAGLLMQKEIANLNKVFEPQRPFLAIVAGAKFDTK
                     IGPLSSLIKIADRLILGGVIYNAYLCSKYKIQIEGVTKEDIEFANKFIIDSKDYLDKI
                     VELPIIVENEGIEKRQGTKELEVQTLKPGTKLNYVLDASAENFTREEIKKVFQEAGTI
                     FVNAVMGYTALYPEGSMAMYSLIDQCPKAIKLFGGGDTIQDFKEYLPGIFAKAQNDPS
                     YYFFTGGGAILDAIQQGSPYGMKPVQMLIRE"
     gene            3470..5371
                     /locus_tag="PLW08_05455"
     CDS             3470..5371
                     /locus_tag="PLW08_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPL52086.1"
                     /translation="MDSEIKLNLLQEFPPPSFEEWLQVVQAGLKGADFDKVLKTKTTE
                     GITLQPIYRQEDIEGIPFLGSQPGSSPFVRGNNPLRFLTEGWLIAQNHTDTDPKILNQ
                     KLLKELNQGLTAVNITLSNKISTEGVKIQNLADWETLLEGINLQAAPLFMQLGIEDRE
                     ILITFVQYAVKHNIELKSLESAIGLDYIGELAEKGYLPCTLADAEQKLAEIVKWNVEK
                     MPRNRIISIDTSIYEEAGASSVQELAFALATAIYYLRQLLNSGVTIEQVAPLLQVKLA
                     LGSNFFMEIAKVRALRLLWAEMIKSFGGEEDKQKVWIHGKTAKFNKTIYDIYVNVLRT
                     TTESFAGVIGGVDSLEIGCFNELIAQPDEFSTRLARNQQIILKEEAHFDKVIDPAGGC
                     YYIEWLTNELAKSAWQLMQEIEGKGGMLEYVTKGEIHQEVETVVRERIEAVNKRKNIF
                     VGINMYANPEEKLPTLQRDDKPVSKEDKAFILKDGALPKHRAVEEIECLRRQIEASQS
                     NKKIFLLNLGTLADYKARADFALNFFPVGGLEVIYPNGFNTVEEAVTAAEKSGASAFC
                     ICSTDENYVSLVPEICAKMKGKILILAGYPADKIEEYKQVGINCFIHLKADVVSTLRD
                     LAKQMEVLK"
     gene            5296..7506
                     /gene="scpA"
                     /locus_tag="PLW08_05460"
     CDS             5296..7506
                     /gene="scpA"
                     /locus_tag="PLW08_05460"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPL52087.1"
                     /translation="MFYSPESGCGIYFKGFGKTDGGVKMKNPDFLQINPDFSATEKPK
                     PKLNFSWKTNEQIEVKPLYTKDDIISTEHLDYLSGLPPFLRGPYPTMYIQRPWTIRQY
                     AGFSTAEESNAFYRRNLAMGQKGLSIAFDLPTHRGYDSDHPRVIGDVGKAGVAVDSIL
                     DMKILFDQIPLDQMSVSMTMNGAVIPIMAFYIVAAEEQGVPPQALNGTIQNDILKEYM
                     VRNTYIYPPEPSMRIIADILSYTAKNMPKFNSISISGYHIHEAGATADLEMAYTLADG
                     LEYVRTALKAGLDIDVIAPRLSFFWAEGMNYFMEVAKLRGARVLWAKIIKQFNPKNPK
                     SMALRTHSQTSGWSLTEQDPYNNITRTCIEALAATMGHTQSLHTNSLDEAIALPTDFS
                     ARIARNTQLYLQNETGICRVIDPWAGSYYVEFLTDALMKSAWKHIIEVENLGGMAKAI
                     ETGLPKMRIEEAAARRQAKIDSGADIIVGVNKYPPETEIPIEILEVDNSAVRDTQIAR
                     LEKLRRERNSEEVEKALQAITKSAETGEGNLLELAIDAARKRATLGEISYAMEKVWGR
                     HQEAIRLINNVYSSEYTKMDEIEQVRVLTDKFAQQEGRRPRILVAKMGQDGHDRGAKV
                     VATAFADMGFDVDVGPLFQTPEETARQAVENDVHIIGISSLAGGHKTLLPKLMEELKK
                     YGREDIMVTVGGVIPPQDYEFLYQNGASFIFGPGTKLPESAKQIMEKLLARGNE"
     gene            7499..8599
                     /gene="meaB"
                     /locus_tag="PLW08_05465"
     CDS             7499..8599
                     /gene="meaB"
                     /locus_tag="PLW08_05465"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPL52088.1"
                     /translation="MSKKRKPEWTPVNSGKEFASEIVTGTKKLKTKTAPPLKEKNYDL
                     KTLVKGVREGNRTLLAKAITLIESNAEKHFLPAQELIKELLPFSGNSIRIGITGVPGA
                     GKSTFIENFGLYLIEQGHKVAVLAIDPSSTISKGSILGDKTRMELLSRNPKSFIRPSP
                     SAGILGGVARKTRETIILCESAGYDVILIETVGVGQSETTVRSMVDFFLLMQIAGAGD
                     ELQGIKRGIIELADLIVINKADGDNIARTELAKRELQNVLHYIRNSTEGWQTKVVTSS
                     ALNKIGLKEIWELITEFQKITKQSSVFAKRRAQQNTQWFENLIAEAVLHRFYQQEKVK
                     ELLPRLKEEVAKGKMPVALAVDTLLKNEKSEE"
     assembly_gap    8685..8784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8785..10690)
                     /locus_tag="PLW08_05470"
     CDS             complement(<8785..10690)
                     /locus_tag="PLW08_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPL52089.1"
                     /translation="MKQRQEKRGLAYQIIVFIFISLFLIFTAFIAITRILFSHIMMEN
                     AQETVSHLANETTYQIENRLTSVVNISRTILTLYRYDHFERNKLDSILHDWVYEFENL
                     EAITIAYAPSYYQEGYSRTIFHSHNRAVYQELHPADYQYLDWFQIPFELKSKYWTEPW
                     YDATATQETVISYCLPLFAEGKCVGIMRLDTKLSALQKIVSPLKLKHSGYAFLVSSIG
                     TIITHPADSLIFNESIFSLAEATNDKNLRQIGKKMINGNIDFVRLQDNAVFGDSWLYY
                     CPLLTNNWSLGIIIPHKDVVGYLNLLLIIQTLFSIIIFLTISFIVYYRTLSVSRPIRL
                     FTEVAERIGQGDFDAKLPKTGNSYEIDRLILAFEKMQESLKDYIKNLEITNAEKNRIE
                     TEVKIASEIQRKLIPENTLQPYEIKELRCYGILEPAGDIGGDLYDFFPIDKSHFLFVI
                     ADVAGKGIVASMTMTMVSTYLRTVSTYHYTAGEIMQNLNNFLCKQTSASNFVTALLGI
                     INLKNGELEFSNAGHTPMIIRKTDLSYKIFTETHSYPLGIFANREIHSTTIKLEKGEE
                     IILVTDGVTETMNGTEDFLGLKGLENIIKNLSSTNPEQTVRQILQQVHSFAQNTPQKD
                     DITILVIDYKK"
     gene            complement(10687..>11495)
                     /locus_tag="PLW08_05475"
     CDS             complement(10687..>11495)
                     /locus_tag="PLW08_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL52090.1"
                     /translation="FYQNKGLNVIIQPGGIKYPPYENLQLGHSDIVQMSLITAVKRDA
                     ENGNLINLAQITQRTSLMLVGKKSRGINSVPDFEGKKIGLWKTDDRLLSLIFLQQNKL
                     NMDIVDLDITINLFLKDGIDVMNVMRYNEYHQIIQAGIDPEDLFSVAFADIGLNVADN
                     GLYTTREFYTKHPKQCRDFASATIQGWFYALNNQEETVSTVLEIMHQYYLPANRPHQK
                     WMLNEICQDFTVHKNQEGILQESDFNKAKKMLTDQNILATQQTYESFFPR"
     assembly_gap    11496..11595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11596..11849)
                     /locus_tag="PLW08_05480"
     CDS             complement(<11596..11849)
                     /locus_tag="PLW08_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL52091.1"
                     /translation="MNNRQIIYLLIALMIAIFFFTSCKQETNVQPEIPEAEAVNTAQN
                     SSLFTIRYRPVWTAQAEFAGVYMAQKKGFYQNKGLNVIIQP"
     gene            complement(12421..14013)
                     /locus_tag="PLW08_05485"
     CDS             complement(12421..14013)
                     /locus_tag="PLW08_05485"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPL52092.1"
                     /translation="MAKYIFVMGGVLSSLGKGIATASIGFLLKSMGYKVVLQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVDEALTKYSSNSAGQIYESVIENE
                     RKGVYLGKTVQVIPHITNEIKGRIQRLANDYDIVITEIGGTVGDIESLPFLEAVRQLR
                     LDLGYYNTLYILLTYVPYIKSAGELKTKPSQHSAYKLREIGIQPDILLCRSEKPFAED
                     IYSKIALFTNVPRENVINAIDVDCVYEIPLNFQKAGLPEIICQHFKLEQRPIIMQDWL
                     QFLENTKNAKETVTIAVCGKYIQHQDAYKSIREALLHAGSALLKKVEIIWIDSERNFK
                     EEDIAKKLEGVQGILVPGGFGVRGIEGKIAVAKYARENNIPYLGICFGMHIAVIECAR
                     NLANLPGASSIEFDEFTPNPVIHLREDQLHRDKVGGTMHLGAFQFNLVKGSKAFKIYQ
                     TETISERHRHRYEFNNDYREVIEKAGLKISGLSPDGLLVEIVEYPQLDFFVAVQFHPE
                     FKSRPINPHPLFREFVKAAAKK"
     gene            complement(14026..15270)
                     /locus_tag="PLW08_05490"
     CDS             complement(14026..15270)
                     /locus_tag="PLW08_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPL52093.1"
                     /translation="MAEKLKLARSMSLFEEAKQLVPGGVAGIRRPYNFVPGEYPIFFD
                     HGKGGRVVDVDGNEYIDFLCAYGPIIIGYREDEIDDAVINQIKNKGFCFSLTQEWQNT
                     LVKKLRELIPCCEMAALVKTGSDATTIAIRVARGYTGKTKIARYGYHGWHDWCVEVKG
                     GIPPKLYEDIYEFHYNDLDSLKAILEANKDDMAGIIITPIGHPNGAEVQMPKPGYLEA
                     VRELANQYHCLLIFDEIRSGFRCSLGGAQKLFGVTPDLSTFGKAMANGYAIAALVGKE
                     EYMQVLADKVFLSSTFFPNSDGIVAAIKTIEILERDRILDVVAAKGRKFGAEVEKVVE
                     ESGVPVNFTGAPWMPYITFKKDEAGLYKKLRTEYYTQLIRRNVFMQPYHHGYICYRHT
                     DEDLAYTVEAIRESLAEVKKML"
BASE COUNT         4864 a         2813 c         3085 g         4597 t
ORIGIN      
        1 gctgcaggtg tgcaaacagc tactttttcc aatttgaaca gcagtactac ctattatttc
       61 aaaatatttc cctatacaaa tagtggaaca aatatcaatt ataagacaga tggaactgtt
      121 cctcagataa gttggactac agcaagtggt cctactatag tagaaattat tttacctcaa
      181 tatatacaag gtaaaaatgg aacaaataac caacgcttac cttgggcttg cagattaaga
      241 ttagaaaatc ttacagctaa tgctacttat agatattttg gacagtttgt tgtttctact
      301 gatgatgcct taaccaatgg tgctggtaat tgctggtttg ttaattcaga taatactatt
      361 actcgcaata caacattagg aatgagcacc gctggaacat attctgaatt tactacaaat
      421 gccaatggtt catatacagg ttggtttata ggtgagccaa cagctaatgt cagatttgct
      481 cccgggaata agctatacta tagaattatg ctcaatgatg gtgcgggagg aacaagcgta
      541 gctatgcgtt taactactgc taattttatt aaggtgatca attttggtac tgaagacaat
      601 gttgaccagg gaacattctt atatggtgtt tctcggtcac cggcaaagaa ctttgtgttt
      661 gtttatgaca atgtagaagg aactggaaga cctatagctg gaagtattat agaaaatgat
      721 ggattagatt tatctgatat tactcaaatt cttgggtctt ataaaaccaa tgtagacaat
      781 gtttctggtg catggggtgt aataattccc aattcagttg gaacaaaagg attttctgga
      841 attaaacgcg ttgaaagtag aaatctttca gatggtagtt tatatgctca agctacagat
      901 agtgatggtg tttggcccag cggagctaat acagtaaatc caagaggggg agatggaact
      961 ccaatagttt taaatgaaaa tgatgctact ctaccggtgg aattatcctc ttttacggca
     1021 aatattaatt cccagggtgg aataaccgtg atgtgggcaa cacaatccga aacgggtgta
     1081 aatggttact atataaatag ggcaacggtt aataacctat ctgaggcatt gaggattagt
     1141 tctttaattc cggcaagcaa tacttctcag cagcaggttt atgtatataa tgataatgaa
     1201 ctctatgaac ccggtacata ttattattgg ttggaaattc aggatattga tggagttgtt
     1261 agttattacg gttcaaggag tgtaactttt ggtggtaatg gtaataatgg aactcctgaa
     1321 attccattgg ttacgggaat tcgttcaatt tatcccaatc cttttaatcc ctcggctaca
     1381 ataatgtatg aacttgatca gccggcaaat gttaatattg aaatatacaa taaccgcggc
     1441 cagatagtgc gtagttttaa cctcgggcaa caagagaaga acagatataa attgctgtgg
     1501 gatggcacag ataattccgg tagtgcttgc ggaacgggaa tatattttgt aaagatgcaa
     1561 gccgggaaag agacctttgt gaagaaagcc gcgctggtaa aatgaagaat gtaaaatagt
     1621 gaaagctaaa agcggaggag agcgttcctc cgttttttct ttcacgcaga ttnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgagagaa atagcttgac gaataacccc
     1801 attttaagat ataaatccaa tttgataata caaaacaata ctctaatctc taaaagtata
     1861 taattaacta tataaaaagg agatacagaa aatgagcaag atagctaata tgctggatgc
     1921 cgatttaaaa ggtaaggtct gtttagtgcg gatggatcat aatgtagtta aaaatggcat
     1981 aattaaggat actatgcgca ttgatgccac aattcctacc ttgctgcata tttacaaaaa
     2041 agggggtttg cctgttttga tgacacatat aggcagaccc tttgataaaa agacaggtat
     2101 tattaacatt tccgaaggag attctgttct gcctgtagtt caatatttgg agaataaatt
     2161 ccaactgcag ggtctaattc cggattgtaa agcggaaagc aagatcggaa taacggattt
     2221 agctcctata ggcattgcga ttgaggcatt aaaaaaggga gcagcagatt ttgtttattt
     2281 acctaatacc cgttggttta aaggggaaga ggcaaaagat gaaagtgccg atatttttgc
     2341 ccaaagttta gctaactatg ccgatgttta tattaacgat gctttcggtt cttggcaggc
     2401 gcatagcagc acctataata ttgccaaatt tcttcctgct tatgcgggat tgctaatgca
     2461 gaaagaaata gcgaatttaa ataaggtgtt tgagccccag cgtccttttt tggctatcgt
     2521 ggcgggagcc aagtttgata ctaaaatagg acctctttcc tctctgataa aaatagccga
     2581 taggctgatt ttaggagggg taatttataa tgcgtacctt tgcagtaaat ataaaatcca
     2641 aattgaaggt gttacaaaag aagatattga atttgccaac aagtttatca ttgatagcaa
     2701 ggactacctt gataaaatag tggaattgcc tataattgta gaaaatgagg gaatagaaaa
     2761 aagacagggg acaaaagaac tggaggttca aaccctcaag ccgggaacga aactaaatta
     2821 tgttctggat gcaagcgctg aaaattttac ccgggaagaa attaaaaagg tgtttcagga
     2881 agcaggaact atctttgtga atgctgtgat gggttataca gctctctatc ctgaaggttc
     2941 tatggctatg tattcattga ttgaccaatg cccaaaagca atcaaactct ttggtggagg
     3001 tgatacaatt caggatttta aagaatatct tcccggtata tttgccaagg cacaaaatga
     3061 cccgagctat tatttcttca ccggaggagg agcaattctg gatgctattc agcagggttc
     3121 accctatggg atgaaaccgg tgcagatgct gatacgagaa tgaagaatgt aaaatggtag
     3181 cacgcagatt tcgcagattt tatttttaca aagagaataa gaggttcaat gaaaaatgta
     3241 taattaagat aggactggta tggaataatt agaaggcgtc ttgttatttt atcaagaccc
     3301 attctatatt ataaatctct taaatccctt taatcctgtt cattccagac ctattatctt
     3361 tttttaatct gcgttatcgg cgaaatctgc gtgcttatat attttcggtg gctaaagaat
     3421 taaggaacga caaggatgtc gttcatctaa tataaaaaag gagaaaatag tggacagtga
     3481 aattaagttg aacctgctgc aggaatttcc tcctcccagt tttgaggaat ggctgcaggt
     3541 agtgcaagca ggactgaaag gtgcggactt tgataaagtt ctgaaaacca aaaccacgga
     3601 aggaataacc ctgcaaccaa tctatcgtca agaagacata gaaggtattc cttttttagg
     3661 aagccaaccg ggaagcagtc cttttgtgcg tggcaataat cccctccgtt ttttaactga
     3721 ggggtggtta attgctcaaa accatacaga tacagacccg aagatactaa accaaaaact
     3781 gctgaaagaa ttgaatcagg gcttgacggc tgtaaatatt accttgagta ataaaatctc
     3841 cacggaaggg gtaaaaatac agaacttggc tgattgggaa acacttttgg aaggaatcaa
     3901 tctgcaagcc gctccgcttt ttatgcaatt ggggattgag gatagggaaa ttctaataac
     3961 atttgtacaa tatgcggtca aacataatat agagttgaaa tccttggaat cagccatagg
     4021 actggattat atcggtgaat tggcagaaaa aggttatctg ccttgcactt tggcggatgc
     4081 agaacagaaa ttagcagaaa tagtaaaatg gaatgtagaa aaaatgcccc ggaacaggat
     4141 tatcagcata gatacctcta tttatgaaga ggcaggtgct tccagtgttc aggaactggc
     4201 ttttgcctta gccactgcaa tttattattt aagacaatta ctgaattccg gtgtgacgat
     4261 agagcaggta gcacctttat tgcaagtaaa attagctctt ggttccaatt tctttatgga
     4321 aatagccaaa gtgcgagctc tgcgtttact ttgggcggaa atgattaagt ccttcggtgg
     4381 ggaagaggat aaacagaagg tttggattca cgggaagaca gctaaattca acaagacaat
     4441 ttacgacatc tatgtaaatg tgttaagaac aacgaccgag agctttgccg gggttattgg
     4501 aggtgtagat agcttggaaa ttggctgttt taatgaacta atcgctcagc cggatgaatt
     4561 ttctacccgt ttagcaagaa atcagcagat tattttaaaa gaagaagcgc attttgataa
     4621 agtgattgac ccagcagggg gttgttacta tatagaatgg ctaaccaatg aactagcaaa
     4681 aagtgcctgg caactaatgc aggaaattga aggcaaaggt ggaatgctgg aatatgtaac
     4741 taaaggagaa attcatcagg aagttgaaac agttgttagg gaaagaatag aggcagtgaa
     4801 taaaaggaaa aacatctttg tgggcatcaa tatgtatgct aatcctgaag agaaattgcc
     4861 taccttacaa agggatgata aacctgtttc aaaagaagat aaagctttta ttctgaaaga
     4921 tggagcatta ccgaagcata gagctgtaga agaaatagaa tgtttaagaa ggcaaattga
     4981 agcaagccaa tccaataaaa agatattttt gctgaatctg ggaactttgg ctgactacaa
     5041 agccagagcg gattttgccc tcaatttctt tcctgtgggt ggtttggaag ttatttatcc
     5101 caatggtttt aatacagttg aggaagcagt aacagcagca gaaaaatctg gtgccagtgc
     5161 cttttgtatt tgttccacgg atgagaatta tgttagctta gttcctgaaa tatgtgctaa
     5221 aatgaagggt aagatattaa ttttagctgg ttatccggca gataaaattg aggaatataa
     5281 gcaggtagga ataaattgtt ttattcacct gaaagcggat gtggtatcta ctttaaggga
     5341 tttggcaaaa cagatggagg tgttaaaatg aaaaaccccg attttctgca aataaatccc
     5401 gattttagcg ctacagaaaa accgaaacct aagctcaatt tcagctggaa aaccaatgag
     5461 cagatagaag tgaaacctct atataccaaa gacgatatta tttctacgga acatttggat
     5521 tatttatctg gtttaccgcc ttttttaaga ggtccctatc ctacaatgta tattcaaaga
     5581 ccttggacga ttcgtcaata tgccggtttt tccacagctg aagaaagcaa tgccttttat
     5641 cgcagaaatt tggctatggg gcagaaaggt ctttccatcg cctttgattt accgacgcat
     5701 aggggttacg attcagacca tccacgcgtg attggagatg taggaaaagc aggagtggca
     5761 gtagattcca ttctggatat gaaaatcctt tttgaccaga ttccgcttga tcagatgagt
     5821 gtttcaatga caatgaacgg agccgtaatt ccgattatgg ctttttatat tgttgctgca
     5881 gaagaacagg gtgtgccgcc ccaggcattg aatggaacta ttcagaatga tattttgaaa
     5941 gaatatatgg tgaggaatac ttatatttat cctccagaac cctcaatgcg tataatagca
     6001 gatattttaa gctacacagc aaaaaatatg cctaagttca atagtatcag catttccggc
     6061 tatcatatac atgaagcggg tgcaactgcc gatttggaaa tggcttatac tttagcagac
     6121 ggtttagaat atgtgcgcac agctttgaaa gccggattgg atattgatgt tatagctccg
     6181 cgtctttcct tcttttgggc agaaggaatg aactatttta tggaagtagc aaaattgcgt
     6241 ggtgccagag ttctttgggc aaaaataatt aagcagttta accctaaaaa tccgaagtca
     6301 atggctttaa gaactcatag ccaaacctcc ggttggagtt taacagaaca agacccctat
     6361 aataatatca ccagaacctg cattgaagca ttagccgcta caatgggtca tacgcaatcc
     6421 ctgcatacta attctttgga tgaagcaatt gccttgccga ctgatttttc tgccagaatt
     6481 gccagaaaca cacagctcta tttacaaaat gaaaccggaa tttgcagagt gatagacccg
     6541 tgggctggtt cttattatgt ggaattttta accgatgctt taatgaaaag tgcctggaaa
     6601 catattatag aagtggaaaa cctgggtgga atggcaaaag cgattgaaac agggcttcct
     6661 aaaatgagga ttgaagaagc agctgccaga aggcaggcaa agattgattc cggagccgat
     6721 attattgtag gtgtgaataa atatcctcct gaaaccgaaa tccctattga aattttggaa
     6781 gtggataact ccgcagtaag ggatacacaa attgcccgtt tggaaaaatt acgccgagaa
     6841 cgcaatagtg aagaagtgga aaaagcactg caggcaatta ccaaaagtgc cgaaacaggt
     6901 gaaggaaacc ttttggaact ggcaattgat gcagccagaa aaagagctac cttaggtgaa
     6961 atatcctacg ctatggaaaa agtttggggt cgtcaccagg aagccattcg tttaattaat
     7021 aatgtttata gcagtgagta cacaaaaatg gatgaaattg aacaagttcg ggttttaacc
     7081 gacaaattcg ctcaacagga aggcagaaga cctcgtattt tagttgccaa aatgggtcag
     7141 gatggtcatg atcgtggtgc taaagtagta gctactgcct ttgccgatat gggttttgat
     7201 gtggatgtag gtcctctatt tcagacccct gaagaaacag cgcgtcaggc agtggaaaat
     7261 gatgtgcata taattggtat cagttctctg gcaggagggc ataaaacact tttacccaag
     7321 ctgatggaag agctgaaaaa atatggcagg gaagatataa tggtaaccgt tggaggggtt
     7381 attcctccgc aggattatga gtttctctat caaaatggtg cctcttttat ttttggaccg
     7441 ggaactaaat tgcctgaatc agccaaacaa attatggaaa agcttttggc acgcgggaat
     7501 gagtaaaaaa cgcaaaccgg aatggacgcc tgttaatagc ggaaaagaat ttgccagcga
     7561 aatagtgaca ggaacaaaaa aactgaaaac taaaaccgca cctccgctta aagaaaagaa
     7621 ttatgacctg aaaacgctgg tgaaaggagt ccgggaaggg aataggactt tacttgctaa
     7681 agccataact ttaatagaaa gcaatgccga aaagcacttt ttacctgcac aggaactgat
     7741 taaagaactt ctgccttttt ccggaaattc cattcgcatt ggcataacgg gagttcctgg
     7801 agcaggaaag agcactttta tagaaaattt tggcttgtat ttaattgagc agggtcataa
     7861 agtagctgtt ttagctatag accccagttc cactatcagc aaaggcagca ttttgggcga
     7921 taaaacaagg atggaattac tttcccgcaa tcccaaaagt tttattcgtc cttctcccag
     7981 tgccggaatt ttaggtggag tagccagaaa aacaagagaa acaattatcc tttgtgaatc
     8041 cgcaggttat gatgtaattt taattgaaac cgtaggagtt ggtcaatcgg aaactacagt
     8101 ccgttcaatg gtagatttct ttttgctgat gcagattgca ggtgcgggag atgaacttca
     8161 aggcattaaa agagggatta ttgaactggc ggatttaata gtaatcaata aagcggatgg
     8221 ggataatatt gccagaacgg aacttgccaa aagagaactg caaaatgttt tgcattatat
     8281 cagaaattca accgagggct ggcaaaccaa agtagttacc tcttctgcct taaataaaat
     8341 tggtttaaag gaaatctggg aactgataac tgaatttcaa aaaattacaa agcaaagcag
     8401 tgtttttgcc aaacgcagag cacagcaaaa cactcagtgg tttgaaaacc tgattgcgga
     8461 ggcggttttg catcgctttt accaacagga aaaagtgaag gaattattgc cccggttgaa
     8521 agaagaagtg gcaaaaggga agatgccggt agcattagca gtggatactc ttttgaaaaa
     8581 tgaaaaaagt gaagaatgaa ggatagaaca cagataacag gattaactgg attagttagc
     8641 ttaataatta taattaaata atcagtatca atgcgctaaa atccnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnntttttt tgtagtcaat caccagaata gttatatcat
     8821 ctttctgggg tgtattttgt gcaaaactat gcacttgctg caaaatttgc ctaacggttt
     8881 gttccggatt ggtagagcta aggtttttaa tgatattttc cagacctttc aatcccagaa
     8941 aatcctctgt gccattcatt gtttcggtta caccatcggt taccagaatt atttcctcgc
     9001 ctttttccag ttttatcgtt gtagaatgaa tttccctatt ggcaaaaatg cctaagggat
     9061 aggaatgggt ttcggtaaat attttatacg acaaatccgt ttttctgatt atcattgggg
     9121 tatgtcccgc gttggaaaat tccagttctc cgtttttcag atttattatt cccaaaaggg
     9181 cagttacaaa attggaagcg gaggtttgtt tacagaggaa attattcagg ttttgcatta
     9241 tttcaccggc agtgtagtga taggtagaaa cggttctcaa ataggtggag accattgtca
     9301 tagtcattga agcaacaatt ccttttcctg ccacatcggc tatcacaaaa agaaaatggg
     9361 atttatctat aggaaagaaa tcgtataaat caccaccaat atctcctgcc ggttccagaa
     9421 taccataaca gcgaagttct tttatttcgt atggctgaag tgtattttcc ggaatcagct
     9481 tgcgttgaat ttcggaagca atttttactt ccgtttcaat acgatttttt tccgcattgg
     9541 taatttccag atttttaata tagtccttca gagattcctg cattttctca aaagccagaa
     9601 taaggcggtc tatttcatag ctattgcctg tttttgggag ttttgcatca aaatcaccct
     9661 gaccaattct ttcagctact tccgtaaaaa ggcggatggg tctggaaacg cttaaagttc
     9721 tataataaac aatgaaggat atagtgagaa agatgattat ggagaaaaga gtttggataa
     9781 tcagcaaaag attgaggtaa cccactacat ctttgtgagg tataatgatt cctaaagacc
     9841 aattattcgt tagcaggggg caataataca gccaggaatc gccaaaaacg gcattatcct
     9901 gtaagcgcac aaaatcaata ttcccgttaa tcattttttt gccgatttgc cttaaattct
     9961 tatcatttgt tgcttccgcc aaactgaaaa tgctttcatt aaaaatgagg gaatcggcag
    10021 ggtgagtaat aattgtacca atactggaaa ccagaaaagc ataaccgcta tgtttcagct
    10081 ttaagggaga aacaattttt tgcaaagcgg agagctttgt atctaatcgc ataattccta
    10141 cgcacttacc ttcggcaaaa agaggtaggc aataggaaat aacagtttct tgggtagcag
    10201 tagcatcata ccagggttct gtccaatatt tacttttcag ttcaaaaggt atctgaaacc
    10261 aatccagata ttgataatcg gcaggatgca attcctggta aacagcccgg ttgtgagagt
    10321 gaaaaatagt gcgggaataa ccctcttgat aataggaagg agcataagca atagttattg
    10381 cttccaggtt ttcaaattca taaacccaat cgtgcaggat agaatccaat ttatttcttt
    10441 caaaatggtc ataacggtat agcgttaaaa ttgtgcgtga gatatttaca acactggtta
    10501 agcggttttc aatttgatag gttgtttcat ttgccaagtg actaactgtt tcttgggcat
    10561 tttccatcat tatatgagaa aaaagaattc tggttatggc aataaacgct gtaaaaataa
    10621 gaaataggga gataaagatg aaaacaatta tctgatatgc cagtccgcgt ttttcttgtc
    10681 tttgtttcat cgggggaaaa agctctcata ggtctgttgg gttgcgagta tattttggtc
    10741 ggtaagcatt tttttggctt tattaaaatc gctttcctgc agaatacctt cctgattttt
    10801 atgcaccgta aaatcctggc atatttcatt cagcatccat ttttgatggg gtctattggc
    10861 tggaagatag tattgatgca taatttccaa gacagtggag acagtttctt cttgattgtt
    10921 aagagcataa aaccagccct gaattgtggc agaggcaaaa tcacgacatt gtttcggatg
    10981 cttagtataa aattcacgag tggtataaag tccattatca gctacattta atccgatatc
    11041 ggcaaaggca acggaaaata aatcctccgg atcaattccc gcttgtataa tttgatggta
    11101 ttcattgtag cgcattacat tcataacatc tatgccgtct ttcaggaaga gattgatagt
    11161 gatatccaaa tccactatat ccatattcag tttattctgc tgtaagaaaa tcaaggatag
    11221 caggcggtca tccgttttcc acagtccaat cttttttcct tcaaaatcgg gtacggaatt
    11281 tatgccgcgg gattttttcc ccaccagcat taaagaagtg cgctgagtaa tttgtgccag
    11341 gtttattaaa ttaccatttt cggcatccct ttttaccgcc gttattaacg acatctgcac
    11401 aatatcacta tgtcccagct gcaaattttc gtagggagga tattttattc ctccaggttg
    11461 gataattaca tttaagccct tattttgata aaaacnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnggttg gataattaca tttaagccct tattttgata aaaacctttc
    11641 ttctgtgcca tataaacacc agcaaattcg gcttgagctg tccacacggg acggtaacga
    11701 attgtaaaca gggaggagtt ttgggctgta tttacagctt ccgcttcggg aatttcaggt
    11761 tgcacatttg tttcttgctt acaacttgtg aaaaagaaaa tggctatcat caaagctatt
    11821 aacagataga ttatttgcct gttattcata aaattacctt tcctccgatg attagcagaa
    11881 agacaataaa tctcatttta tgtaacctct taccgttcag cttatgaccc gaactttaat
    11941 ccatctcata ccttatttac ttatatcgct aattactatt ttttgtcaat aagaaacaag
    12001 cagaaaaatt gatctgattt gttgatttat ttccacttct ctcttccaac ctgattttta
    12061 ggcgctctta ctccttcagt taataaccaa gtaacaacca agtaacaact ccgtaacgga
    12121 actacggaag tgttacggaa gtgttacgga gtcgttactt aatcaactat ataccaagca
    12181 aataactatc aacattcaac gggcaaaaga gaaaagcaaa aatgaatgac agaaggtaga
    12241 taaatgaatg aattggattc accgaacttt cagccaccga aattagcgaa agaagataaa
    12301 aacacgcaga tttcgcagat aacgcagatt tttattttat aattatcctg atattttaat
    12361 ccagctaatc cagtagctcc agttatctgc gttctattta tttctttcct tcttccttct
    12421 tcattttttt gctgcggctt taacgaattc tctaaatagc ggatgcggat tgattggtct
    12481 gcttttgaac tccggatgga attgaactgc cacaaagaaa tccaattgag ggtattctac
    12541 aatttctacc aaaagtccat ccggagataa accacttatt ttaagtcctg ctttttctat
    12601 tacttcacga tagtcattat tgaattcata gcgatgtctg tgcctttcgc tgatagtttc
    12661 tgtttgatag atcttaaatg ctttactgcc ttttaccaaa ttaaattgaa aagctcctaa
    12721 atgcattgtc ccgccaactt tatcccgatg taattgatct tcccttaaat ggatcacagg
    12781 attgggtgta aattcatcaa attcaatgct actagcaccc ggtaaattag ctaaatttct
    12841 ggcacattca attaccgcta tatgcattcc gaaacagata cccaaataag gaatattatt
    12901 ttcccgggca tatttagcaa cagcaatttt gccttcaatg cctcttacgc caaaaccacc
    12961 tggaacaaga attccttgaa ctccttccag tttcttagcg atatcttctt ccttaaaatt
    13021 gcgttcgcta tctatccaga taatttccac cttttttagt aaagcggaac cggcgtgtaa
    13081 aagtgcttct ctaatgcttt tataggcatc ctgatgttga atatattttc cgcaaacagc
    13141 aattgtaacg gtttctttgg catttttggt gttttctaaa aactgcagcc agtcctgcat
    13201 aatgattggt cgctgttcta acttaaaatg ctgacagata atttccggta aacctgcttt
    13261 ttgaaaattg agtggaattt cataaacaca atccacatca atggcattga ttacattttc
    13321 tcttggcaca ttggtaaaaa gagctatctt gctataaata tcttcagcaa aaggtttttc
    13381 acttcggcaa agcaaaatat ccggttgaat tcctatttcc cgtaatttat aagcactatg
    13441 ttgtgagggc ttagttttca attctcctgc cgatttgatg tatggaacat aagttaaaag
    13501 aatatacagc gtgttgtaat aacccaggtc taaacgcaat tgacgcactg cttccagaaa
    13561 aggtaaactt tcaatatcac caaccgtccc tccaatttcc gtaatcacga tgtcataatc
    13621 atttgctaaa cgctgaattc taccctttat ttcgtttgtg atatgcggaa taacttgcac
    13681 tgtcttgcct aaatatacac ctttgcgttc attttctatc acactttcat atatctgacc
    13741 tgcagaattg ctggaatact tcgttaacgc ttcatccaca aaacgctcat aatgccccaa
    13801 atccaaatcc gtttctgctc catcatccgt tacaaaaacc tcgccatgct gaaaaggact
    13861 cattgttccc gggtcaacat ttaagtaagg atcaaacttt tgaagcacaa ctttatagcc
    13921 cattgatttt aacaaaaagc caatagaagc ggtggcaatg cccttcccta aagaagaaag
    13981 cacgccaccc attacaaata tatattttgc catttagctt atctcttata acatcttttt
    14041 cacttcggca agggattctc taattgcttc caccgtgtat gccaaatctt catccgtatg
    14101 ccgataacaa atatagccat gatgataggg ctgcataaaa acattgcgcc taattaactg
    14161 ggtataatat tctgtccgca actttttata aagccccgcc tcatcctttt taaaggttat
    14221 ataaggcatc cagggagctc cggtaaaatt aaccggcact cctgattcct caactacttt
    14281 ttccacttcc gctccaaact ttcttccttt tgcagcaacc acatccaaaa tacggtctct
    14341 ttccaatatt tctatggttt ttatagcagc tacgatgcca tcgctattgg ggaaaaatgt
    14401 tgaggataaa aataccttat ctgccaagac ctgcatatat tcctcttttc ctactaaagc
    14461 tgcaatagca taaccatttg ccattgcctt tccgaaagtg gataaatcag gtgttacccc
    14521 aaataatttt tgcgctcctc ctaaactgca tctaaaacca ctgcggattt catcaaaaat
    14581 taacaaacag tgatattgat ttgccaattc acgcactgct tccagataac ccggttttgg
    14641 catttggact tccgctccat taggatgacc tataggagta ataattatcc ctgccatatc
    14701 atctttattg gcttccagaa ttgcttttag ggaatccaaa tcgttgtaat gaaattcata
    14761 aatatcttca tacaacttgg gaggaattcc acctttcact tctacacacc aatcgtgcca
    14821 gccatgataa ccgtaacgtg ctattttagt tttgcctgta taaccgcgcg ctacacgaat
    14881 agcaatagtt gtagcatcac tgccggtttt tactaaagca gccatttcac aacagggtat
    14941 cagttctctc agctttttca ctaaagtatt ttgccattcc tgagtgagag aaaaacaaaa
    15001 acccttgttc ttaatctgat tgataacagc atcgtcaatt tcatcttcac ggtaaccaat
    15061 aataataggt ccgtaggcac aaagaaagtc aatgtattcg ttaccgtcca catcaacaac
    15121 tctgccgcct ttgccgtgat caaagaaaat gggatattca cccggaacaa aattgtaggg
    15181 tcttcttatc ccggcaacgc ctccgggaac aagttgcttt gcctcttcaa aaagagacat
    15241 acttctggct aatttcaatt tttctgccat tttatatact ccttagttaa gttcaaagtt
    15301 caaagtttag cacgcagatt tcgcagattt cgcagataaa aaagataatg ggtctgggat
    15361 gaacaggatt aaagggattt aagagattta taatatagaa tgggtcttga taaaataaca
    15421 agacaccttc tacttttcct tttattccac accaattcta tcttatttat gcattctata
    15481 ttaaatcact aattctctta tctctttaaa aaatctgcgt aatctgtctc ttatcattat
    15541 acattctaca ttttacattc tcaattattc tcttatcctc ttttctcttt gtaaaatatc
    15601 tgcctaatct gcgtaatctg cgtgctatca ttctacattt cccacagggt cgctattcc
//
LOCUS       DAOOZV010000043        14915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4057_length_14915_cov_63.526649, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000043 DAOOZV010000000
VERSION     DAOOZV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_14915_cov_63.526649"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1428)
                     /gene="mnmE"
                     /locus_tag="PLW08_05495"
     CDS             complement(55..1428)
                     /gene="mnmE"
                     /locus_tag="PLW08_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPL52094.1"
                     /translation="MSKISEVICAPVTPLGFSAIAVIRLSGKGCIELVANHFSQSQKL
                     LSSPSHNLIFGTFYAETGEPIDEVLISVFREPHSYTGEDVIEISCHGNPNLVNRILQT
                     LLLSCRLAKPGEFTLRAFLNGKMDLSQAEAVNDLIQAQANQAEKAALMQLKGFLSKYL
                     QELLARITELRIRFELAIDFADQDLPLPDSNALYQELLDIIKTAEELKSTGEQGRRLR
                     EGIKICLTGAPNVGKSSLFNALLQQNRAIVTPHPGTTRDYLEEYLSLNGFPIVIYDTA
                     GLREFPDDIEKEGITKSYELMQESDLILYLVEATTVTNPNLQFSDLLSPSSLPPDLHS
                     KTLVVFSKADLIQENTPQISRGIYCSVISENGLKDLTDAISKRLMLNTELPNKPIIIN
                     NRHLVALSKCLQSLHRAKQCLKENQGYEFIAFELISASNALEEILGVITTDDLLDKIF
                     SEFCIGK"
     gene            complement(1421..3289)
                     /gene="dxs"
                     /locus_tag="PLW08_05500"
     CDS             complement(1421..3289)
                     /gene="dxs"
                     /locus_tag="PLW08_05500"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPL52095.1"
                     /translation="MILEQITEPKQIQALTVSELKILAKEVRSRIVEVVSKTGGHLAP
                     SLGTVDLTIALLHLFDPLKDRIVWDVGHQAYSWKILTGRNAQFDTLRQYKGLSGFTNR
                     EESPYDAFTTGHSSTSISAALGIACARDLNNEQGHCIAVIGDGALTGGMSFEALNHGG
                     HLQKDKFIVILNDNEMSISKNVGGLQKYMARMLASKSYNVLKKQVWDFSYTLPANIRR
                     SFIYGAQKLEESMMNILVPNIIFEDLGYKYVGPIDGHDIEHLLKIIKRVKNFMVGPVL
                     IHIVTQKGKGYPPAEKNSELFHGTGPFDLKTGKQISNGKQTWSELMGSTLITLAQKDP
                     KIIAITAAMTAGTGLTKFEETYPDRFFDVGIAEQHSVTLAAGMSTKGLKPFVAIYSTF
                     LQRALDQIIHDVALPKLPVVFCIDRAGLVGEDGATHHGAFDLSYLNFVPNMIILTPST
                     AEELSAMLFWAANYQEGPVAIRYPRGTAIHSSVHKSLTNFNPFPAIIHSNKGKIALVA
                     CGDAFFTAEAVYNLLAKDKIPAQLIQLLSVKPLDENTLLSLASNCNYIFTFENNAVIG
                     GMGARIAQLLATEPVKVINFGYPDHFIAQGKNSELLDEIGFTPNKLHKQIVQTLNE"
     gene            complement(3309..5225)
                     /locus_tag="PLW08_05505"
     CDS             complement(3309..5225)
                     /locus_tag="PLW08_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPL52096.1"
                     /translation="MFQLSFLNASLLFFAIATILPLLIWLLAKKKPQKIIFPTLRFIK
                     LSKEQEKSRSKLKNILLLIIRMLIILLVVLAVSRPMLSSPKLKPSGKHPPTAIAILID
                     TSYSMDYAERGKSSLQYAKDALKKINSKANPDDRLIPVSSDENWNLLHSQIYAGSLPE
                     TLIDQLKITFHPLSLEEMLALAETKLAESQMPNREIYLISDLRIPPAELKTNIPIALI
                     PLPETSEYENLAVISANALPQLVEKHNQQLIQFTVANYGSTKRQDVLLKAVVNDIKLA
                     EKFISIPSQSTITETIPVELRSDGWQSGYIEVNDERQIMDNRCYFTFPFYSKPRVAVI
                     SQSSNLPPILATVLRVYSSSTPQVISPDALSLNDLDKFQLFIVYNCGPLTSRLLEIFT
                     TLQNRKSGILFCLGNNLSADLKSYLNNTFGLEIKDRTQKSITIDNINAHHYISSLVSG
                     KPLKNPILADYWQAVNKSAGVLISAQNFPLAVINGKQSLWLWNISADNNPFFIDPAFP
                     VLAFRTLDYIAGSEIPETNAKIGQVLTFNRLKLPTGEIITSRRYLATEPGIYIFEPDT
                     PRSYAMAINIDYSDSEARTNFPKGVKRLGEKWEDKLFFSRLGHDLWKILLAIAFALMI
                     VEIIIVKTEEARAK"
     gene            complement(5226..6272)
                     /locus_tag="PLW08_05510"
     CDS             complement(5226..6272)
                     /locus_tag="PLW08_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52097.1"
                     /translation="MPKSLIYIILLISPAWLLLAQQVTPLPITSYDYALPANNVSPIA
                     MGTGALNLTNPEDPFCAYGNPALMADNKLGSLSLSFLLSTQEDLEFYEALSLSNTLKD
                     KQFKYFVLNTGPVSFSYQPVSAVHISQFTNNGMNSEYYDYKMDKVQISWGGRDENYPK
                     FAAGINLKYLSGRLVYLKEHKEGYNLIRDGFIDDKAKGISGDLGFTYKMGNIVYSASF
                     YDLLSMLWWENYDSVTLKRRAALGLGSEGTNSHYFAGIQGKIAQEPETTFHFGYSYTF
                     NFGKSNYSTTQNRNSLDLRVGLYSHDFYGTDNINFTLGGGYYYKNFRFDFSLNNTGMK
                     LSDSDYLFSLSAGI"
     gene            complement(6293..7228)
                     /locus_tag="PLW08_05515"
     CDS             complement(6293..7228)
                     /locus_tag="PLW08_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPL52098.1"
                     /translation="MTDTSITANRKTSFFLSAIDLITLIFCGWILLYMCFGITRSPEV
                     IKHIPVYLAIFVGVLFLAWLQKQPGWSYDPQNPSKRYQILSFFRGLYPVLLFGYFYTS
                     GHAFNRIIFRDWLDPFFMGIDQFIFGYLPSLVWGKLYSYWAIQELFHFAYFCYYPMIA
                     GIPIYLYFTQKDAFREVIFNLTFVFYCCYTIYSVLPVIGGRFLPEAMALTKTYRGGPF
                     THIMVFIYRTSNHLGGAFPSSHIAIAIVLTISALKYIRPLGYICTVITFFLSLATVYC
                     HYHWFIDAVFGILTGIAGYYLANWTYYYLREKGFN"
     gene            complement(7239..8366)
                     /locus_tag="PLW08_05520"
     CDS             complement(7239..8366)
                     /locus_tag="PLW08_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL52099.1"
                     /translation="MINVIPVDNKKKLKDFILLPFKLYKNEPNWVPPLIGDQKKFFNP
                     KKNPYYYHSEVKLFLALKDGEPVGRISAHSNTQHNKEHNENIGFFGFFECIDNQEVAN
                     ALFDAAFEWNRYRNFTSLRGPMNFSVNQDCGLLINGFDSPPMIMMPYNFPYYPKLYEN
                     YGLTKTMDLYAFISEYHSIPERVAKMAEIIAKRTNVQIHSLSYDKKQRKKDIETVFEI
                     YTRAWQYNWGNVPMTKAEFDHLVAELLPIADPDMVFIAEVDGQLAGFSLTLPNYNEVL
                     KVMQGKVNPITIIKALQAKKHITSVRVITMGIIKEFQGKGIDTLLYYHTFKNGLPKGY
                     YRSEFSWILENNIPMINAAEKLGAKIYKTYRLYDKAITNRK"
     gene            complement(8388..9032)
                     /locus_tag="PLW08_05525"
     CDS             complement(8388..9032)
                     /locus_tag="PLW08_05525"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPL52100.1"
                     /translation="MIYLFVILIIIIAYLYGCFSTARIVAKSFRSLNVYKIGTGLADT
                     ENIYTNISKPLGTLVGAIDVAKASLFLKIVEFLLGFIDIKVINYPDFTILYSENLMLI
                     YGLSMLIGHCLPLTHRFRGGRGIFTYMGILAYIAFLPTLISAVIAWLIVLHFKQIRFA
                     QYLIVILPVILYIIFYTLFHYHESSPPYFITLLLGNAIMMGILNIIVSKKLGEF"
     gene            complement(9029..9730)
                     /locus_tag="PLW08_05530"
     CDS             complement(9029..9730)
                     /locus_tag="PLW08_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPL52101.1"
                     /translation="MNIAENIKHLQDNIADILSRLNRKDEITLVAVTKTHPVEIIETA
                     LQSGIEHIGENRVQEARRKIPFLKVPYKGFHFIGHLQSNKINQVLELKPFLIQSVHSL
                     ELAKKLHLALGRTNRTQDILIQINSSQEFSKNGFSFAEVTNAICQISTYSNLHIKGLM
                     TIGMLAEPEVSRPLFARMKSLFEEIKALAIPNVDMQYLSMGMSNDYVQALEEGSNMLR
                     IGTAIFGERSYGVNE"
     gene            complement(9727..13347)
                     /locus_tag="PLW08_05535"
     CDS             complement(9727..13347)
                     /locus_tag="PLW08_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL52102.1"
                     /translation="MKKVLVLGSAILICLSLAAFNTEINIDPFAWQNKKTAEELPVTI
                     NPGEPVLPFTPVNILLPFGHRYLNSTVQLSTAELVAKNITFAFAGPQLPISDQVFTNS
                     FPPANTIPENRLYPSQQWQFLGTQYFRGYQIALFNVYPYRYNPVTQKLYVSSQISISI
                     NSEFSEDEACYQANFYTPGTKTLQIINSLICNPDKIPSYQLANNYRYVTVPNRIIDLS
                     TPKQMIIITNNARIGWFSEYSAWRSAKGVSNSIYSMEDIISAYPGVDNAEKLRNFIIN
                     AYQTWANSSQPLEYVILGGDDEIVPERGAYGQVGDTVDARMPTDIYFSNLDGNWNANQ
                     NQIWGEINDNTDLIPEVHIGRFPAETQTEFNNMFRKIQYYVDNNTYSNNYAVFMGENL
                     NNNPLTWGGDYKDDVAQYLPDEYYLQTLYQRDGTYSADNVYNAISNGAGIMNHMGHSN
                     EVSLIGQSNTTVESLTNTEYGFLYSQGCYPAAFDQRTSGNNEAIGEHFVTAAGALFSF
                     IGNTRYGWYSPGNINGASQFYDRQFFRGMFQQNCPVLGDALTFSRLQNLNSALSNDVM
                     RWCYYEVVLFGDPSIAIKPYDPNLPLLNLESYTFSDEEGDNDGIINPGEIIRFYPLIS
                     NNINLADATNVSLHIENLPPGVELLSDDIIIPQILAGNQSPANIYFRIQLSDDMSFGI
                     YSFNLVLNSQHPITFQSTGAHRFPIFFQITLIDNRFPWETIVNGKSAPLVADLNSSPG
                     LEIIYADVYGNSYIIGSNGELLNTLEAPSGMLINRSFASGPINSDDQNDLVFCSRSGN
                     IFALRLNGDIIFNYHTDTSFLFTPVLADIDGNGTLETIVGGINGKLYAVSSSGQLMNG
                     FPFELGGIINSELAAADFDGNGSFEIVAGSTNGLLTVIGANGIVRNGFPVQLNGPVTG
                     FPTITNTNRIVCSTPTDIYIISPEGNIIATRNINTHITGGFAVGNITADNTGTDIAGV
                     TLNGILYAFTDSGIDLPGFPVNIGENFACPPLLVNLDSNPQLEIIVHNYMNSVYVYKA
                     DGSPLSGFPFINIYNGSTPATLVDFDNNNQTKLVLGFSNGVLMLNLRRNTAHLGPWIT
                     YRGSALRQGSFASTGFIGSSDEYNIPALNALLGNYPNPFNPETTICYTTKANVQAKLE
                     IFNIKGQKIRTLIDAIHSEGNHSIVWNGYDDFSRKVASGVYFYRLNIEGKTFQRKMLL
                     LK"
     gene            13592..14353
                     /locus_tag="PLW08_05540"
     CDS             13592..14353
                     /locus_tag="PLW08_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL52103.1"
                     /translation="MPIPFNMNRLKDTEIHYPQRFSNMLTKNYGLLFYNEGNKASQES
                     NHAVILDLIGVESSLRDIEFFYKSKGIHPCIYPALTNKELEILTPLLDEHNFTLKIKQ
                     SDFFLHDHESSLRESEELKIERIHQLDIDIMETIAIEYGGDWTIRVVERHLTHPSYHF
                     LGGYYQGELATMASVSIFAGYSRVDDVFTREKFRGKGFAGGMIHHLINYHRNISQNYL
                     YLQSDLPSAIKIYEKAGFSRLPKDFQFWVAYKEHN"
     gene            complement(14702..>14915)
                     /locus_tag="PLW08_05545"
     CDS             complement(14702..>14915)
                     /locus_tag="PLW08_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52104.1"
                     /translation="DGGWNAMYLGLKAGYVLPLVENVYVDFGLKYQMGLGDYGGDDYE
                     DLPDEYKENKESTFGFNLGLQIFFSK"
BASE COUNT         4801 a         2606 c         3159 g         4349 t
ORIGIN      
        1 acaataaata agtcaaggta tttctaaatt gcagttaact tcgctgcgga tagcttattt
       61 tccgatacag aattcgctga agattttatc caagaggtcg tcggtggtga taactcctaa
      121 aatttcttcc aaagcattgc tggcagaaat aagttcaaaa gcgatgaatt cgtagccctg
      181 attttctttt aggcattgtt ttgcccgatg caaggattgc aagcatttag atagagcaac
      241 taaatgtctg ttattgatga taattggttt attgggcaat tcagtgttca gcattaaacg
      301 cttggaaatt gcatcggtaa ggtctttcaa accattttcc gatattacac tgcaatatat
      361 cccgcgggag atttgaggtg tattttcttg tattaagtca gctttgctga aaacaacaag
      421 ggttttggaa tgaagatcag ggggaagaga agagggagaa agtaaatcgg aaaattgtag
      481 atttggatta gttacggtag ttgcttccac cagataaaga ataaggtctg attcctgcat
      541 caattcataa cttttagtta ttccttcttt ttcaatgtca tcaggaaatt ctcttagtcc
      601 agcagtatca taaataacta tgggaaagcc atttagggat aaatattctt caagatagtc
      661 cctggtagtt cccggatggg gagtaacaat ggctctgttt tgttgcaata aggcattaaa
      721 aagtgaggat ttaccaacat ttggtgcacc agttaaacaa attttaattc cttcccgcaa
      781 gcgtcttccc tgttcacctg tacttttcaa ttcttcggcg gttttgataa tatctaaaag
      841 ttcttgatac agtgcattgg aatcgggtaa aggtaaatcc tgatcggcaa aatcaattgc
      901 cagctcaaaa cggattcgca gttccgtaat gcgggcaagc aattcttgta agtatttgct
      961 cagaaaaccc ttaagttgca taagagctgc tttttctgct tggtttgcct gggcttgaat
     1021 aaggtcatta actgcctctg cctggcttaa atccattttt ccgtttaaga aggcacgtaa
     1081 agtaaattct cccggttttg ccaaacgaca ggaaagaagt aaggtctgaa gaatacgatt
     1141 tacaagattg gggtttccgt ggcaggaaat ttctataaca tcttcacctg tataactatg
     1201 tggttctcta aaaacggaaa taagaacttc gtctatcggc tcaccggttt ctgcataaaa
     1261 ggtgccaaaa atcaaattat gcgaaggtga agaaagaagt ttttggcttt gggaaaagtg
     1321 attggcaaca agctcaatac agcccttgcc acttaagcgg attacggcaa tggcagaaaa
     1381 tccaagcgga gtgactggag cacaaataac ttcgcttatt ttactcattt agtgtttgaa
     1441 caatttgttt atgaagcttg ttaggcgtaa aaccaatttc atcaagaagt tcagaatttt
     1501 ttccttgagc aataaagtga tcgggatagc caaaattgat aactttaact ggttcggttg
     1561 caagcaattg agctattcgt gctcccatcc ctccaataac ggcattgttt tcaaaagtga
     1621 agatataatt gcagttggaa gccaaagaca aaagggtatt ttcatccagg ggtttaacgg
     1681 aaagtaattg tatcagttga gcaggaattt tatctttagc cagtaagtta tacactgcct
     1741 cggctgtaaa aaaagcatct ccgcaagcga ctaatgcaat ttttccttta ttggagtgga
     1801 ttatggcagg aaagggatta aaattggtta aagatttatg aacagaagaa tgaattgctg
     1861 ttccacgagg ataacgaata gcaacaggac cttcctgata atttgcagcc cagaataaca
     1921 ttgcagaaag ttcttcagcc gtagaagggg ttaaaataat catattcggc acaaaattca
     1981 agtaggaaag atcaaaagca ccatgatgtg tagctccatc ttcacctaca agaccagctc
     2041 tatcaataca aaaaacaacc ggtaatttag gtaaagcaac atcgtggata atctggtcta
     2101 aagcacgttg cagaaaagtg gaataaattg ctacaaaggg tttaagtcct ttagtggaca
     2161 ttcctgcggc aagggttacg gaatgctgtt cagcaattcc aacatcaaaa aagcgatcgg
     2221 gataggtctc ttcaaatttg gttaaaccag ttcctgcagt catagcggct gtaatagcga
     2281 ttattttggg gtctttttgg gctaaagtga ttaaggttga acccatcaat tcgctccagg
     2341 tttgttttcc gttgctgatt tgtttaccgg ttttgaggtc aaaaggacct gttccatgaa
     2401 ataattccga attcttttcc gctggaggat aaccctttcc cttttgggta acaatatgga
     2461 tcagaacagg accgaccata aagttcttta ctcttttgat aattttcaaa agatgctcaa
     2521 tatcgtggcc atctatcgga cctacatatt tatagccaag gtcttcaaaa ataatgttgg
     2581 gcaccaggat attcatcatt gattcttcca gtttctgagc tccataaata aaactgcgac
     2641 gaatattggc tggtaaagtg taactgaaat cccaaacctg ctttttaagg acattatagg
     2701 atttactagc aagcatccga gccatatatt tctgtaatcc gcctacattt ttggagatgg
     2761 acatttcgtt atcattcagg ataacaatga atttatcctt ttgcaggtga cccccatgat
     2821 ttaatgcttc aaaactcatt cctccggtta aagcaccatc tccaataaca gcaatacagt
     2881 gaccctgttc attgtttaaa tccctagcac aggcaattcc taaggcggca gaaatggaag
     2941 tgctgctatg acctgtagta aaagcatcat aaggactttc ttcacgattg gtaaatccgc
     3001 tgagaccttt atactgtctt aaggtatcaa attgagcatt gcgtccggtt aaaattttcc
     3061 agctgtatgc ttgatgccct acatcccaaa cgattctatc tttaagcgga tcaaaaagat
     3121 gaagcaaagc aattgtgagg tctacagttc ctaaactggg agctaaatga ccgccagttt
     3181 tggagacaac ttctacaatg cgagagcgaa cctccttagc caggattttc aattcgctaa
     3241 cggttagagc ttgtatttgt ttcggctcgg ttatttgttc caaaatcatt ttttctcctt
     3301 gccgcctgct attttgccct tgcctcctct gtttttacaa taataatttc cacaatcatt
     3361 aaagcgaaag cgatggcaag caaaattttc cagaggtcgt gacccaagcg ggaaaagaaa
     3421 agtttatctt cccatttttc tcctaatctt tttacacctt tagggaaatt agttctggct
     3481 tcggaatcgc tgtagtcaat atttattgcc atagcataac tgcgaggagt atcaggttca
     3541 aaaatataaa ttccgggttc ggtagctaaa taacggcggc tggtgataat ttcacctgtg
     3601 gggagtttca atctgttaaa agtaagaacc tgaccaattt tagcatttgt ttccggaatt
     3661 tcagaaccgg caatataatc taatgtgcgg aaagccagaa ccggaaaagc cgggtctata
     3721 aaaaaaggat tattgtccgc agaaatattc cagagccaga gtgactgctt gccgttgatt
     3781 acagctaaag gaaagttttg agcagaaatg agaactccgg cacttttatt tactgcttgc
     3841 cagtagtcag ccagaatagg atttttaaga ggtttcccgg aaacgaggga actgatatag
     3901 tgatgggcat taatgttatc aatagtgatt gacttttggg tgcggtcttt tatttctaag
     3961 ccaaaagtgt tgttcagata ggacttcagg tcggcagaaa ggttatttcc caggcagaaa
     4021 agaatacctg atttacggtt ttgtaaagta gtaaaaatct caagtaaacg agaagttaaa
     4081 ggaccacaat tgtatacaat aaagagttga aatttatcca ggtcatttaa tgagagggca
     4141 tcaggcgaaa ttacttgagg agtagaagag ctataaaccc ttaaaacggt agccagaata
     4201 ggaggtaaat tgctactttg agagataact gcaacccggg gtttactgta aaaggggaaa
     4261 gtgaaatagc aacggttatc cattatctgg cgttcatcat taacttcaat atatccactc
     4321 tgccagccat cggaacgaag ttcaacagga atagtttcgg tgatagtgct ttgggaaggg
     4381 atagagataa atttttcggc aagttttata tcgtttacta cagctttaag gagaacatct
     4441 tggcgtttgg tgctgccata attggcaacg gtgaactgaa ttaattgctg attgtgtttt
     4501 tccaccagct ggggtaaagc attggcagaa ataactgcta agttttcata ttcagatgtt
     4561 tcaggtaagg gaataagagc aataggaata ttggttttaa gttctgcagg aggaattctt
     4621 aaatcgctga taagatatat ttcccgattg ggcatttgac tttcggcaag tttggtttct
     4681 gccaaggcaa gcatttcttc caaagagaga gggtggaaag tgatttttaa ttggtcaatc
     4741 agcgtttcag gcaaggaacc ggcataaatt tgactgtgca aaaggttcca gttttcatcg
     4801 gaactgacag gaatgaggcg atcatcggga ttggctttgg aatttatctt tttcagagca
     4861 tctttggcat attggaggga actttttcct ctttcagcat agtccatact gtatgaagta
     4921 tcaattaaaa tggcaattgc tgtaggtgga tgttttccgg aaggttttag tttcggggaa
     4981 gagagcattg gacgcgaaac ggctaaaaca accagaagga taatcagcat cctgataatc
     5041 agcaagagta tgtttttcag cttgctgcga cttttctcct gttctttgct caatttgatg
     5101 aaacgtaaag tagggaaaat aattttttgg ggcttttttt ttgccaacag ccaaatcagc
     5161 agaggcagaa tggttgctat ggcaaaaaag aggagagaag catttaggaa agaaagctga
     5221 aacaattata ttcccgcact taaagaaaaa agatagtcgc tatcggagag cttcattccc
     5281 gtgttattca gcgagaaatc aaagcggaag tttttgtaat aatagccgcc accaagagta
     5341 aagttaatat tatccgttcc gtaaaaatcg tgactgtaaa gaccaactct gaggtcaagg
     5401 gaattacggt tttgggtagt agagtaattg cttttgccaa aattaaaggt gtagctgtag
     5461 ccaaaatgaa aagtggtttc tggctcttga gcaattttgc cttgtatacc ggcaaaataa
     5521 tggctgtttg tgccttcaga accaagtccc aaagcggctc ttcttttaag ggttacggaa
     5581 tcataatttt cccaccagag catagacagg aggtcataaa aggaagcgga ataaacaata
     5641 ttacccattt tgtaagtgaa accgaggtct ccggaaatac ctttagcttt atcatcaata
     5701 aagccatcgc ggattaggtt gtaaccctct ttatgttctt tcaagtaaac gaggcgtcct
     5761 gaaaggtatt taaggttaat tccggcagca aatttgggat aattttcgtc tctgccaccc
     5821 caagaaattt ggactttatc cattttataa tcgtaatatt cactgttcat tccgttgttt
     5881 gtgaactgac tgatatggac agcagaaacg ggttgataac tgaaagagac aggaccggta
     5941 ttgagcacga agtatttgaa ctgcttatct ttcagggtat tgcttaagct aagagcttca
     6001 tagaattcaa gatcttcctg agtgcttaaa aggaaggaaa gggaaagcga gccaagtttg
     6061 ttatctgcca ttaaagcagg attgccgtag gcgcagaaag ggtcttcagg attggttaaa
     6121 tttaaggcac ctgttcccat agcaataggg gagacattat tggcagggag ggcgtaatca
     6181 taagaagtta taggtaaagg agttacctgt tgagcaagta ggagccaagc gggagagata
     6241 agtaagatga tataaatgag tgatttaggc attagaaatt ttttcttgga catcagttga
     6301 accctttttc tctaagataa taataagtcc agttagctaa atagtaaccg gcaatacctg
     6361 ttaagatacc aaaaacggca tcaatgaacc agtgatagtg acaataaacg gtagcaagag
     6421 aaaggaaaaa ggtaatgaca gtgcaaatat agccaagcgg acggatatat tttagggcgg
     6481 aaatagttag aacaatggca atggcaatat gactgctggg gaaagcacct cccaaatgat
     6541 tggaagtgcg atagataaag accataatat gagtgaaggg accaccccga taggttttgg
     6601 ttaaagccat agcttcagga agaaaacgtc caccgataac aggcagaaca gaatagatgg
     6661 tataacaaca atagaagaca aaggtgagat tgaagattac ttccctgaaa gcatcttttt
     6721 gagtgaaata gagatagatg ggaattccag caatcatagg ataatagcaa aaataggcaa
     6781 aatgaaaaag ttcctgaatt gcccaatagg aatagagttt tccccaaaca agagaaggca
     6841 ggtaaccaaa gataaattga tcaattccca taaaaaaggg atcgagccaa tcccgaaaaa
     6901 taatacgatt gaaagcgtga ccagaagtgt aaaaatagcc gaaaaggaga acaggataga
     6961 gacctctgaa aaaactgaga atttgatagc gcttggaagg gttttgagga tcgtaagacc
     7021 atccgggttg tttttggagc caggcaagaa aaagaactcc tacaaaaata gcaagataaa
     7081 cagggatatg ttttataact tcaggagagc gagtgatacc aaaacacata tatagaagta
     7141 tccatccgca aaagataagg gtaataagat caatggcaga gaggaagaaa gatgtttttc
     7201 tatttgccgt tatggaggta tccgtcattc agaacctgct atttcctatt agtaatggct
     7261 ttatcgtaaa ggcggtaggt tttataaatt ttggcaccga gtttttcagc ggcattaatc
     7321 attgggatat tgttttccag aatccaggaa aattcgctgc ggtaatagcc cttggggagt
     7381 ccatttttaa aagtatgata atagagcaaa gtatcaattc ccttaccttg aaattcctta
     7441 ataataccca tagtgataac gcggacggag gtgatatgtt ttttagcttg gagagctttg
     7501 ataattgtaa taggatttac tttaccctgc ataactttta agacctcatt gtaattgggt
     7561 aaagttaaac tgaaaccggc aagctgacca tcaacttcag caataaaaac catatccgga
     7621 tcggcaatag ggagtaattc agcgacaagg tgatcaaatt cagcttttgt cataggaaca
     7681 ttgccccagt tatattgcca ggcacgggta taaatttcaa agacggtttc aatatctttt
     7741 ttgcgttgtt ttttatcgta ggacagggaa tggatttgaa cattagtgcg tttggcaatt
     7801 atttctgcca ttttggcaac gcgttcggga atggaatgat attcactaat gaaggcatag
     7861 aggtccattg ttttggttaa gccgtagttt tcgtaaagtt ttggataata aggaaagtta
     7921 tagggcatca taatcatcgg gggggaatca aagccattga ttaataatcc gcaatcctga
     7981 ttgacactga aattcatagg accgcggaga gaagtaaaat tacgatagcg attccactca
     8041 aaagcggcgt caaaaagggc attggcaact tcctgattat caatacactc aaagaagcca
     8101 aagaaaccga tattttcgtt atgttctttg ttatgctgag tattggaatg agcagaaatt
     8161 cttcctacgg gttctccatc tttaagagca agaaaaagtt taacttcgga atggtaataa
     8221 tagggatttt tcttggggtt aaagaacttt ttctggtcac caattaaagg aggaacccag
     8281 ttgggttcat ttttgtataa cttaaaagga aggagaatga agtccttcag cttttttttg
     8341 ttatctacag gaattacatt gatcattgct ttacctcatt aggggattta gaattctccc
     8401 agttttttag aaacgatgat atttaaaata cccatcatta tggcgttgcc aagtaaaaga
     8461 gtaataaaat agggagggga agattcatgg tagtgaaaca aggtgtaaaa aataatatat
     8521 aaaataaccg gcaaaatcac aatgagatat tgagcaaacc ttatttgctt aaagtggaga
     8581 acaataagcc aggcaataac agcagaaata agagttggaa gaaaagcaat gtaagcaagt
     8641 atacccatat aagtaaaaat tccgcgtcca ccccgaaaac ggtgagttaa aggcaagcag
     8701 tgtccaataa gcatactgag tccataaatc aacattaggt tttccgaata gagaatagtg
     8761 aaatcgggat agttaataac tttgatatca ataaaaccca ggagaaattc cacaatcttc
     8821 aaaaacagag atgctttagc tacatcaatg gctcctacta aagttcccag gggcttgctg
     8881 atattagtat aaatattttc agtatcagca agaccggttc caattttata aacattgaga
     8941 gaacggaaac ttttggcaac gatacgagca gtggaaaaac aaccgtaaag ataggcgatg
     9001 ataataataa gaataacaaa aaggtagatc attcattaac tccatagcta cgctctccaa
     9061 aaatggcagt gccaatgcgt aacatattgg aaccttcttc caatgcttga acatagtcgt
     9121 tactcatacc catagagaga tattgcatat ctacattagg aatagcaaga gcttttattt
     9181 cttcaaatag ggatttcatt ctggcaaaaa gaggtctgct gacttcaggt tctgcgagca
     9241 taccgatagt catcagtcct ttaatatgca agttagaata ggttgagatt tggcaaattg
     9301 cgtttgtaac ttccgcgaag gaaaagccgt ttttggagaa ttcttggctg ctattgattt
     9361 gaataaggat atcctgtgtg cgattggttc tacctaaagc cagatggagt ttttttgcca
     9421 gctccagaga atggaccgat tgaattaaaa agggcttaag ttccagaacc tgatttattt
     9481 tgttgctttg gagatgaccg ataaagtgaa aacctttgta aggaacctta agaaagggaa
     9541 ttttccttcg tgcctcctgc actctgtttt cgccgatatg ttcaatacca gattgcaaag
     9601 cggtttcaat tatttctaca ggatgagttt tggtaacggc aaccaaagta atttcatctt
     9661 tacgattcaa acgggacaaa atatccgcaa tgttatcctg caggtgttta atgttttcgg
     9721 ctatattcat ttcagcagta acatcttgcg ttggaatgtt tttccttcaa tgttcaagcg
     9781 atagaaatag acaccgctgg caacttttct ggaaaagtcg tcatatccgt tccaaacaat
     9841 gctatgattg ccttcactat gaatagcgtc tatcaaagtg cggatttttt gacctttgat
     9901 attgaaaatt tccagtttgg cttgaacatt ggcttttgta gtgtagcaaa ttgtagtttc
     9961 cggattgaag ggattgggat aatttcccag taaagcgttt aaggcgggaa tattatattc
    10021 atcggaagaa ccaataaagc cggtggaagc aaaagaaccc tgccttaaag cacttccccg
    10081 ataggtgatc cagggaccta aatgggcagt attacgacgc aggttaagca ttaaaacgcc
    10141 attagaaaaa cctaatacca gtttggtttg gttattgtta tcaaagtcaa ctaaggttgc
    10201 tggtgtgcta ccattgtaaa tattgataaa ggggaaaccg cttaaaggac tgccatccgc
    10261 tttgtaaaca taaacagaat tcatataatt atggacaatt atctctaact gagggttact
    10321 atccagattg acaagaagag gaggacaagc aaaattttct ccaatattta caggaaaacc
    10381 aggaaggtca ataccggaat cagtgaaggc atagaggata ccgttgagag ttacacctgc
    10441 aatatctgtt cctgtattat cggcagtaat atttcctacg gcaaaaccac cggtaatgtg
    10501 agtattgata ttacgggtgg caatgatatt tccctcggga gaaatgatat aaatatccgt
    10561 gggtgtagaa caaacgattc tattggtgtt agtaatagtt ggaaagccgg ttacgggacc
    10621 gtttaattga acaggaaaac cgtttcggac aatgccatta gctccaatga cagttaaaag
    10681 accgttagtg gagccggcta caatttcaaa agagccattg ccgtcaaaat cggcggcagc
    10741 aagttcgcta ttgataattc ctccaagttc aaaaggaaag ccattcatca gttgtcccga
    10801 ggaagaaacg gcatacaatt ttccgtttat gccaccgaca attgtttcca gggtgccgtt
    10861 gccatctata tcagccaata caggagtgaa taaaaaagat gtatctgtat gataattaaa
    10921 gatgatatct ccgtttaacc gaagagcgaa aatatttcct gagcgagagc agaaaaccag
    10981 atcgttttga tcatcactgt taatgggacc gctagcaaaa ctacgattga ttaacattcc
    11041 tgaaggggct tcaagggtat taagcaattc tccgttacta ccaattatat agctgtttcc
    11101 ataaacatct gcataaataa tttcaagacc gggactggaa ttcaggtctg ccacaagagg
    11161 tgctgactta ccattaacta ttgtttccca gggaaaacga ttgtcaataa gagttatctg
    11221 gaagaaaata ggaaatctat gtgcaccagt tgattgaaaa gttataggat gctgagaatt
    11281 aagcaccaga ttgaaactgt atattccaaa actcatatca tcagaaagct ggatacggaa
    11341 ataaatgtta gcaggacttt ggtttccggc aagaatttga gggataataa tatcatcgga
    11401 gagcaattcc actccgggag ggagattttc tatgtggaga gaaacattgg ttgcatcagc
    11461 caaattgata ttattggaaa taagaggata gaaacggata atttcaccgg gattaatgat
    11521 accatcatta tcgccttctt catcagagaa agtatagctt tccaaattta ataggggaag
    11581 attgggatca tatggtttaa tagcaataga agggtctccg aacaggacta cttcgtaata
    11641 acaccagcgc ataacatcat tgcttaaggc agaattcagg ttttgtaagc gggaaaaggt
    11701 taaggcatca cctaaaacag gacaattctg ttgaaacata cctcggaaaa actggcggtc
    11761 ataaaattga gaagcaccat tgatgtttcc aggactatac catccgtaac gagtgttgcc
    11821 aataaaagaa aataaagctc cagcggcagt aacaaaatgc tcaccgatag cttcattgtt
    11881 tccggaagtg cgttgatcaa aagctgcggg ataacaaccc tgagaataga gaaaaccata
    11941 ttcagtatta gttaaacttt caacggtagt atttgattga ccgataagag aaacttcgtt
    12001 ggaatgaccc atatgattca taattcctgc accgttactg attgcattgt aaacattatc
    12061 ggcgctgtaa gtgccatctc tctgataaag ggtttggaga tagtattcat caggaagata
    12121 ttgagctaca tcatctttat agtcgccacc ccaggttaag ggattattgt tcaggttttc
    12181 acccataaaa acagcatagt tgttgctgta ggtattatta tccacataat attgaatttt
    12241 tctgaacata ttattgaatt cagtttgggt ttcagcagga aatctaccga tatgaacttc
    12301 cggaatcagg tctgtattat catttatttc accccagatt tgattttgat tagcattcca
    12361 attgccatct aagttgctaa aatatatatc ggtaggcatt ctggcgtcaa ctgtatcacc
    12421 aacttgacca taggcgcctc gttccggaac aatttcatcg tcaccaccta aaataacata
    12481 ttcaagtggc tgggaactat tagcccaggt ctgataagca tttataatga agttacggag
    12541 tttttccgca ttgtcaactc cgggataggc agaaatgata tcttccatag agtaaataga
    12601 attggaaaca cctttggcag aacgccaggc agaatattca gaaaaccagc cgatcctggc
    12661 attattggta ataattatca tttgtttggg tgtggaaagg tctataatgc gattaggaac
    12721 tgtaacatag cgataattat ttgccaattg ataggaggga attttatccg gattgcagat
    12781 aagggaattt attatttgga gagttttagt gcctggagta taaaaatttg cctggtagca
    12841 agcttcatct tcagaaaatt cactattaat agaaatgcta atctgggagc tgacatacag
    12901 tttttgcgtt acaggattat agcgataggg atatacatta aacaaggcaa tttgatagcc
    12961 ccgaaagtat tgtgttccta aaaactgcca ctgctgagaa ggataaagac ggttttccgg
    13021 aattgtgttt gcaggcggaa aagaatttgt aaatacctga tcagaaattg gcaattgagg
    13081 tccggcaaag gcaaaagtaa tattttttgc aaccagttct gcagttgata actgaacagt
    13141 ggaattaaga tatctatgac caaaaggaag cagaatattt accggagtaa aaggcaaaac
    13201 cggttctccg ggatttattg ttacaggaag ttcttcagcc gtttttttat tttgccaggc
    13261 aaaaggatca atgtttattt ctgtattaaa agcagccaaa ctgagacaaa tcagaatggc
    13321 actaccaagg acaaggacct ttttcatagt ttatatccat cttttttatt agttacttat
    13381 aaaaagtagc aagatgtatt ccttgatcat aaaacatatc aattcgtcaa gtattttcct
    13441 gaaaatagag tattaaattc cgattcggca agtggcaaag gggaatttgc cacttcattt
    13501 tctttaagca aggaactatt tatttgctct tttactaaaa ccgtctaatt tattattaaa
    13561 agaactttta cttatgagaa aggagtaaac tatgccaata ccttttaata tgaaccggtt
    13621 gaaagatacg gaaattcatt atccgcagcg tttttccaat atgttaacca aaaattacgg
    13681 acttcttttc tacaatgaag gaaacaaagc ttctcaggaa agcaatcatg ccgtgatttt
    13741 agatttgata ggggttgaga gttctttgag ggatatagag tttttctata aaagcaaagg
    13801 tattcatccc tgtatctatc ctgcattaac taataaagaa ctggagattt taacacccct
    13861 gctggatgag cataatttta ccctgaagat caagcaaagc gatttttttc tgcatgatca
    13921 cgaaagcagt ttaagggaat ccgaagaact gaaaattgaa cgcattcacc aactggacat
    13981 tgatattatg gaaacaattg ctatagaata tggaggtgac tggacaatta gggtagtgga
    14041 gcgtcatcta actcaccctt cctatcattt tttgggtgga tattatcagg gtgaactggc
    14101 aacaatggct tcagttagta tctttgccgg ttacagtaga gttgatgatg ttttcaccag
    14161 agaaaaattc agaggaaagg gttttgcagg aggaatgatt catcatttaa taaattacca
    14221 ccggaatatc tcacagaact atctctacct gcaatcagat cttccttccg ctattaaaat
    14281 ttacgaaaag gcaggttttt cccgtctgcc caaggatttt caattttggg tggcttataa
    14341 agaacataat tagagtaagt gctgaatagt gcatcaggga acagaaaggc aattttacag
    14401 tatagggtaa gaaaatcggg cgttgcttca aagaatataa ctttcagatt aaaaatacat
    14461 acctattttt attttctgtg gattggggtt gtcacacaac ctcccaaaag tccatttttt
    14521 gccattttta ggtgtatcct aaagtggata ttgttcctta aaatggctta tatttactgt
    14581 ctcaaaacta acttttagtt tcgggacagc cccaatgaca aaggacttat cattcaagaa
    14641 atgagataaa aaaatccccg gaaaaccggg gatagtattg tttttggatt tttacggtta
    14701 attatttgga gaagaatatc tgcaatccaa ggttaaagcc aaatgtgctt tctttatttt
    14761 ccttgtattc atcaggtaaa tcttcataat catctccgcc ataatctcct aaacccattt
    14821 gatatttgag tccgaaatcc acatatacat tttcaacaag aggcagaaca tatccagctt
    14881 tcaagccaag atacattgca ttccatccac catca
//
LOCUS       DAOOZV010000044        14913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4059_length_14913_cov_54.792031, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000044 DAOOZV010000000
VERSION     DAOOZV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14913
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_14913_cov_54.792031"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(550..6012)
                     /locus_tag="PLW08_05550"
     CDS             complement(550..6012)
                     /locus_tag="PLW08_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL52105.1"
                     /translation="MKTTKLAILILMCSFSTLFAGTIIPIEKPLSVVSQNAGKIKLQL
                     QVPELQIEDIDNSNFKILSMQGAETTGETGFPELPVFSAWIAIPPKGDIEIKVTGGEI
                     ITQKGFIPKPVFATKEQEIASEYNKTAYHSASLYPANSYSYSQPQIIRDFRVVQITLN
                     PVQYVAETQELKIQKQMEVEIEVKDRQGINEMDEYNGYSYAFTNLYESMIANFDYYRN
                     TMMAPQPARILLIYGNNTDAIFIAKLNEFVAWKRQKGYDVNFVSTVETGGASTQAIKN
                     YIQTQYDNPSTRPDFIILLGDTNGSYAIPTYTEQMSTYGGKGDYPYTFLAGNDYLGDA
                     FIGRISAENISQLDVLFTKIYAVEKNINVSGNAGAWVNKMLLIGDPGNSGISTIYVNK
                     FIKELAKRTNPDYTFLENYSSGFPAYIDNGFNQGVAFFNYRGWLGMSGWSPSASLING
                     NKLPHSTILTCSTGNFENNTATSESLIRLGTSAAPAGAITAIGMATSGTHTTFNNCLN
                     AGIYDGIFTYGMRSMGEALLNAKLYIKQVYGSTFDQQANYFAHWCNLMGDPTVEVFTQ
                     IPDIFNLACADILPLGSNLLDVEVTNSFGTGVKDACVTLYSTAQNIIVGRDYTDSEGK
                     ATINISGGMLNELTVTVSKHNYKPFQKVVTIDPTGSLVYLNKIVIDNGTNGSYGNGDS
                     FATAGETIALSLEIKNTTSATVAATTAILSADDPYITILNSESSFDAIDSNATLWSNS
                     VFTFSIVNNLPAQHNVRFTLQLTTADQNVYSFVFHTVIYNALLLVHNSSISAGGNNIL
                     DPGENGFLSLTIKNNSIAPVFDVYAELSSLNDLITVTDSTSYVGSILGNSLGTTVEGF
                     EVFARPLLIPGMQMPLRLHLYNEWGFDQYTEFNIQIGSVNSHTPLGPDAFGYFIYDIT
                     DTAYPDCPVYEWIEINPSLGGAGTKITAFSDPGSSTDEGDQTGAVTLQQIDLPFTFPF
                     YGIDYNQITVCVNGFIVMGFTENGEFRNYHLPGGYGPSPMLAPFWDDLCLINDAGIYK
                     YYDSQNHRFIIEYFKMRNGYNRSSLETFEVIFYDPVFYPTSMGQGMVKFQYQDFNNVD
                     IGQGGYSPSHGNFCTIGIKDHTNTIGLEYTFNNQYPSAAAPITNFSALLITTAPVLYE
                     SPFLVLNEVIINDTNFNQIAEPGETIELGIKLMNIGLGTATQVQLSAFCASEYAVLIN
                     AISNYSNILGDATAINNTPITLQIDANCPNAAEIPINLHISISDNSWDFTVYITVHKP
                     DIQYSGMYMNDSITGNGNGLIDPGETLDLIINFANNTPVSANNISSTITCLSEYVTVH
                     NNSCIISSLPAGGISQAIYNITISPNVIVGNNLTFYLTYLADFTSVHSEQLVLSVGTT
                     GMNADFENNNGNFVPNPSSNGWEWGESTVAGAHSGTKVWGTRLNSTYPNNANYQLTTP
                     SVYIGSNFMLEFWHYYNSEANYDGGNVKISTNGGTTWTLLTPVGGYSNTNLSVLNGPG
                     YSGNSGGWVFARFNLSIYANQSVQFRFTFASDSGITGDGWFIDDVRTTGFVEFAGKVF
                     GTVTSSNSAIDFSIVNVQNSASWISHPDNEGNYAIYLPIGSHSIWASADGYKNSTSAV
                     ITISLANPLVQQDFYLGYFTPVSGISYMLADSVLTLNWNPPSEPEFPLIHYEIYRRIN
                     AGAFALAGITVIPVYTETLRELGTDYYFYVVCVYSEGSSMPSEILHYNYLTDIEDNVN
                     PALITQLLNNYPNPFNPETTIRFTLQETAPAKLYVYNIKGQLVKKLIDEVLPSGMHQI
                     VWNGKDNNNRNVASGMYFYRLESKNYSCIKKMLLLK"
     gene            complement(6109..7131)
                     /locus_tag="PLW08_05555"
     CDS             complement(6109..7131)
                     /locus_tag="PLW08_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52106.1"
                     /translation="MPKSYIITLAIIVLILLSSCAKRNTEYQEGDWLTLRNVLPIVGN
                     PLDIKIDDNYIYVAHDQGGMSRISRTDYHIQWITEFWAEDGSQKVLGRIKKVAVVPEH
                     NFMFLVDTEATDAIRVINTSDPDSLIYILDFIGGTSNIKDLDSFAITPVPFLQGTYKM
                     VCAYCAAGSFKYDYYDGTIFNSNVFTISPGAAAGFKLTDNYIFIAAEQLGLYIYNRND
                     RQLIGNIHLSGEAQKVEVNNNIAYVAGRQSGLHIVDVSNPASPVLLSTFDTSGYATSL
                     AVSGNKLALSSGGGGIYLFDVSIPSKPQLLQHLTSCGYTNTVKFMDNKLVVGARDQGI
                     LIYEIK"
     gene            complement(7495..9144)
                     /gene="hcp"
                     /locus_tag="PLW08_05560"
                     /gene_synonym="priS"
     CDS             complement(7495..9144)
                     /gene="hcp"
                     /locus_tag="PLW08_05560"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPL52107.1"
                     /translation="MSMFCYQCQETSRNLGCTMRGVCGKSETLANLFDLLIYGLKGLA
                     YVSVKLQEKDRYYPEDSVFVMSALFKTITNVNWDEENIKDTIETTLAVRDKRKDELCK
                     LMEGKCDTCPEAVTFQLKRDEYDEFAKKVGVLRTENEDIRSLRETIIYGIKGICAYGE
                     HAAILGFQDDDVNKFIMEGLSATLDDNLTVDQLVNLVLRTGENAVKVMAKLDEANTKT
                     YGNPEPTKVKLGVGKNPGILISGHDLKDLEELLKQTEGKGVDIYTHGEMLPAHYYPAF
                     KKYKHFIGNYGDSWWHQLEDFENFNGAILMTTNCLVPLRKENTYLERLFTTGTVNYPG
                     AVHIPDREKGREKDFSPVIARALKCQPPKELETGEITGGFAHSTVLSLADKIIEAVKS
                     GAIKRFVVMAGCDGRIPSRKYFTEVAEKLPKDTVILTAGCAKYRYIKLPLGDINGIPR
                     VLDAGQCNDSYSLAVIALKLKEAFGLDDVNKLPLSFDLAWYEQKAVAVLLALLSLGFK
                     NIILGPTLPGFLSPNVAKVISDTFGLQQITDADSDIAKMLA"
     gene            complement(9161..9478)
                     /locus_tag="PLW08_05565"
     CDS             complement(9161..9478)
                     /locus_tag="PLW08_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPL52108.1"
                     /translation="MKSRTWQDVEPKVNANGILGSKIYEAPEGEIVHLNLAPGAHLKK
                     HITPVNVAFYVLEGTATLEIGEEKQSFPEDTLIESPKDIPHGVFNEGNSNLRLLVIKM
                     PKP"
     gene            complement(9488..10075)
                     /locus_tag="PLW08_05570"
     CDS             complement(9488..10075)
                     /locus_tag="PLW08_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL52109.1"
                     /translation="MELTERQKDIIMAGIAIIANQGYEKLTTKNLATKIGVTEAALYR
                     HFKSKRDLVTTILGYFEELSNKVLQEIREKDYSPLESIRHFVEDRYILFSEHPNLAKV
                     MFSEELFKNDPTFKGQFQCIMHKHKQVMENYIIQAQQEGKIRSDLSPTQLFRIIVGSM
                     RFTVTQWNLSDGAFDLKKEGSELIETIIKLIETKQ"
     gene            10411..11481
                     /gene="hydE"
                     /locus_tag="PLW08_05575"
     CDS             10411..11481
                     /gene="hydE"
                     /locus_tag="PLW08_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPL52110.1"
                     /translation="MRPDKNKLIELLSTEGEENLQTLYNQAYKVKKKYVGTTVYLRGL
                     IELSNICSKNCYYCGIRSGNKELKRYQISYEEAIAEAKLCQQMHYGSIVIQAGERNDK
                     PWIDFITKLIIGIKELSEGKLGITLSLGEQKREVYQQWFEAGAHRYLLRIETSNEELY
                     KSLHPAAQSFETRLKCLHSIKECGYQVGTGVMIGLPGQTIEDLANDILFFYDEDIDMV
                     GMGPYIPHHNTPLKHLVDSFNPAEALKMGLKMIAACRIALKDVNIASTTALQALHPEG
                     RELGLKAGANVLMPNITDTKYRKGYQLYEGKPGLNETAIQYQKELEQRISALGETIGY
                     DEWGDSKHFYSREVQRSMKNEK"
     gene            11616..11939
                     /locus_tag="PLW08_05580"
     CDS             11616..11939
                     /locus_tag="PLW08_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPL52111.1"
                     /translation="MLPGGKNMQQLMKQAQKMQAEIMKQQEELANTVFSASSGGGMVT
                     VEMNGQFEVVSLKIDPQVVDPEDVEMLEDLITAAVQEVLNKVNEASSNVMSKVTGGIN
                     IPGLG"
     gene            11940..12539
                     /gene="recR"
                     /locus_tag="PLW08_05585"
     CDS             11940..12539
                     /gene="recR"
                     /locus_tag="PLW08_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPL52112.1"
                     /translation="MLFSENLEKLIQSFNRFPGIGRKTAQRLAWYLVAQDKNFALNLA
                     ETIKTTVESFKTCSQCNMLSESDPCPFCISPDRSDSYLCVVENSSDIQILEGMNEFKG
                     RYFVLGHLLSPLDGYGPEQINTAMLLERIENLHPGEVILALKPSAEGEATIHYLWELL
                     KDKGISITRLSTGIPFGGDLEYSNLNTLANAWKRRYTVQ"
     gene            12508..13131
                     /locus_tag="PLW08_05590"
     CDS             12508..13131
                     /locus_tag="PLW08_05590"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPL52113.1"
                     /translation="MPGNADIPFSNMFTGIIEATGKIVTHQLISGKKLITISRPAIFD
                     DLKIGCSIACDGICLTVVQFDDKAFTVEIMNETLQKSTAKNWTSGKILNLERALKLNS
                     RLDGHFVQGHIDRELRLISHLKREGTDYLHLELPSQERNLIVPQGAIALNGVSLTIAE
                     IRNNYFSVALIGHTLTHTNLGTIKTGEKVNVEYDILGKYVLAQIPKG"
     gene            13175..14458
                     /locus_tag="PLW08_05595"
     CDS             13175..14458
                     /locus_tag="PLW08_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPL52114.1"
                     /translation="MRFNKTFICLFFLLIILFLISGCGNKRSPTGGPQDIDKPVVLES
                     SPAEFGDISSGYIEISFSKPMDRNTLANSIYIYPPVQNKKITLDGANLKIHIKEPLKP
                     DTNYFITLTKRLKDLRGNTLEKNQTLIYSNGKLATNRIAGTITYEEPTDKGLPLELTL
                     LSADSLLVLSSVIQGGAYAIDNLNPQKHILRTYIDKDFNGRYDFGREPFFEGITDGSS
                     VATLNITMAYGDTTRAKISQVNVLSDRELQVILSEDIKSYDALNIQTKNTPLSIAYQL
                     IEGYIINLLTAKMDSTEYTLTLTGVKDKKDNISNKLETKFKVRPVADTLAPKIIFTNP
                     RNGASVNTLLPVLEIHFNEIIPKANIKAKLLCGNQEIPLKHLSETGRIHRFQPPKELE
                     NYKSHLLIISSETTDFSGNHLKNDYELQFLPLLRK"
BASE COUNT         4803 a         2824 c         2640 g         4646 t
ORIGIN      
        1 ctcttttttt aagaaaaagt tccgtaggaa cgacagattt tatagaaggg ttttaaccct
       61 taaaaaaaaa tagggaaaat aagaatattt taccaagata agaaatacaa tataatagat
      121 aggaaagcta ctctgataag caaaataaaa ttatttaaca tccaggaaag gatttaactc
      181 tattattctt ataaataaaa ttcaaaagca gagataggat gatcaggttg tttttattgg
      241 taatacagcg taaagaatct aattatgcag tcgtaaatgt tattttttca aaaaaagggc
      301 attcttgctt ttccttttat cgtataaata tagaatcttt cattaccgta taatttgtgg
      361 gattgttata gtgcggttgt aagtgtgctt gctttctgtt ttaactcaca attttttatt
      421 tgggaaacta actataacgc gattggaagt gaaaaaacaa gaggactgtt tataattggg
      481 aaagcaggtg aaaattgtga gtcaaaacct tttccaagtt ttctctggtt tccctattaa
      541 attcactctt catttcaaga ggagcatttt ttttatgcag gaatagtttt tactttccag
      601 ccggtagaaa tacattccgg aagccacatt gcggttgtta ttatcttttc cgttccagac
      661 aatttgatgc attcccgaag gtaaaacttc atctatcagc tttttcacca gttgtccttt
      721 aatgttatat acataaagtt tagcaggagc cgtttcctgc aaagtaaatc tgatagttgt
      781 ttccggattg aaaggattag gataattatt taacaattga gttataagag caggattcac
      841 attatcttca atatctgtaa gataattgta gtgcaaaatc tctgaaggca tactgctacc
      901 ctccgaataa acgcaaacca cataaaaata ataatccgtg cctaattctc ttaatgtttc
      961 cgtataaaca ggtataactg taattcctgc caatgcaaaa gctccggcat taattctacg
     1021 ataaatttca tagtgtatta aaggaaattc cggttcagaa gggggattcc agttaagagt
     1081 taaaacgcta tcggctaaca tataactaat tcccgaaacg ggagtaaaat aacccagata
     1141 aaagtcctgc tgaactaaag ggttagccag agaaattgtg atcacagcag aagtagaatt
     1201 tttatagcca tctgcagaag cccaaattga atgcgagcca atagggagat aaattgcgta
     1261 gtttccttca ttatcaggat gactaatcca ggaagcagaa ttttgcacat taactatgct
     1321 gaaatcaata gcagaattac tgctggttac cgttccgaaa acctttcccg caaattcaac
     1381 aaaacctgta gtgcgaacat catctataaa ccatccatct cctgtaatac cactatcact
     1441 cgcaaaagta aaacggaatt gaacactctg attggcataa attgaaagat tgaaacgggc
     1501 aaaaacccag ccaccactgt ttccactata acctggtccg tttaacacac tcaaatttgt
     1561 gtttgaataa ccacctaccg gagtaagcaa agtccaggtt gttcccccgt ttgtagaaat
     1621 tttaacattg cccccatcat aatttgcttc tgaattataa taatgccaga attccagcat
     1681 aaaattgctg ccgatataaa cactcggagt agtaagctgg taattagcat tattaggata
     1741 agtactattc aatcttgttc cccagacctt agttccggaa tgagctcctg caacagttga
     1801 ttcaccccat tcccaaccgt tggatgaagg attgggaaca aaatttccgt tattattttc
     1861 aaaatcggca ttcatacctg tagtgcctac acttaaaacc aattgttcac tatgaacact
     1921 tgtaaaatca gccaaatatg ttagatagaa agtaagattg ttcccaacta tcacattcgg
     1981 agaaatagtt atattatata ttgcttggct tattccccct gcaggtaagg aagaaataat
     2041 gcaggaattg ttatgaacag taacatattc cgaaaggcaa gtgattgtgc tgctaatatt
     2101 attagcagaa acaggagtgt tattggcaaa attgataatc aaatcaagtg tttcacccgg
     2161 atcaataagt ccgtttccat ttcctgtaat actatcgttc atatacattc cactatattg
     2221 tatatcaggt ttatgaacag taatataaac agtaaaatcc cagctgttat ctgaaatgct
     2281 gatatgcaag ttgattggga tttcagctgc attgggacaa ttggcatcaa tttgaagagt
     2341 aattggggta ttatttatcg ctgttgcatc tcctaaaata ttactgtaat tgctgatagc
     2401 attgatcaga acggcatatt cgcttgcaca aaaagcactt aactgaactt gtgtagctgt
     2461 gcctaatcct atattcatta gtttaatacc cagttcaatt gtttctcccg gttcggctat
     2521 ctgattaaag ttggtatcat ttataattac ttcattcaat accagaaaag gtgattcgta
     2581 tagaactgga gctgtagtaa tcaacagagc tgaaaaattt gttatgggag ctgctgcact
     2641 gggatattga ttgttaaaag tatattccag ccctatagta ttcgtatgat ctttaattcc
     2701 gatagtgcag aaattgccat gcgaaggact gtatcctccc tgcccgatat ccacattatt
     2761 gaaatcctga tattgaaatt taaccattcc ctgtcccatt gaggtaggat agaaaacggg
     2821 atcataaaaa ataacttcaa aggtctccag agaagaacga ttgtatccat tacgcatttt
     2881 gaaatattct ataataaacc tgtgattttg gctatcgtaa tatttatata tccctgcatc
     2941 attaattaaa cataaatcat cccagaaagg agcaagcatg ggagaaggtc cgtaaccacc
     3001 tggaagatga tagttccgaa attcgccatt ttccgtaaac cccattacta taaagccatt
     3061 tacgcaaacg gtaatttgat tgtagtcaat gccataaaag ggaaaagtga aaggcaaatc
     3121 tatttgctgt aaagtgaccg ctccggtttg atctccttcg tcagttgaag aaccgggatc
     3181 ggaaaaagcg gttatttttg ttcccgctcc tcccaaggaa ggattgattt ctatccattc
     3241 atacacagga caatcaggat aagcggtatc ggttatatca taaataaaat agccgaaagc
     3301 atcgggacct aaaggtgtgt gagaattaac actacctatt tgaatgttaa actcggtata
     3361 ttgatcaaag ccccattcgt tatataggtg taaacgcaac ggcatttgca ttccaggaat
     3421 caataaaggc cgggcaaaga cctcaaaacc ttctacagta gtccccaaac tgtttcctaa
     3481 aatggaaccc acataagaag tagaatccgt aacggttatc agatcgttca aagaagataa
     3541 ttcggcataa acatcaaaga caggagcaat ggaattattt ttaatggtta aggaaagaaa
     3601 accattttca cccggatcaa gaatattatt tcctcctgca gatattgaag agttgtgaac
     3661 aagcaacaaa gcattataga taacagtatg aaatacaaag gaatatacat tttgatcagc
     3721 tgttgttaat tgcaatgtaa aacgcacatt atgctgagca ggtaaattgt ttactatgct
     3781 gaaagtaaat acagaattag accaaagcgt ggcattactg tcaatagcgt caaaactgct
     3841 ttcagaattc aaaatagtaa tataaggatc atcggcagaa agaatggctg ttgttgctgc
     3901 aactgttgct gaagttgtat ttttaatttc caagcttaga gctatggttt ctcctgcagt
     3961 ggcaaaacta tcgccattac cgtaactgcc attagtaccg ttatcaataa ctattttgtt
     4021 taaataaaca agggaaccag tagggtcaat tgttacaact ttttggaagg gtttataatt
     4081 atgtttggaa acagtaaccg tcagctcatt tagcatcccg cctgaaatat tgatagttgc
     4141 cttaccttca ctgtcggtat aatctctgcc aactataata ttctgggcag tactataaag
     4201 tgttacacag gcatctttta ctcccgtccc aaaggaattt gtaacttcca catccaaaag
     4261 attactgcct aaaggtaaaa tatctgcaca agcgaggtta aaaatatcag gaatttgagt
     4321 aaaaacctct accgtagggt ctcccattaa attacaccaa tgggcaaaat aattcgcctg
     4381 ttgatcaaaa gtagaaccat aaacctgctt aatgtataat ttggcattta acaatgcttc
     4441 tcccatactg cgcataccat aagtaaaaat cccgtcataa attccggcat tcaaacaatt
     4501 gttaaaagta gtatgagttc cactagtagc cattccaatt gctgtaattg ctccagcagg
     4561 agctgcagaa gttcctaaac gaataagaga ttcggatgta gcggtgttat tttcaaaatt
     4621 accagtgcta caagtaagaa ttgtagaatg aggtagctta ttgccattga ttaaactggc
     4681 ggatggactc caaccgctca ttcctaacca accgcgataa ttgaaaaagg caactccctg
     4741 attaaaacca ttgtcaatat aggcagggaa tccgctggaa taattctcca gaaaagtata
     4801 atcgggatta gttctttttg ccaattcctt gatgaattta tttacataaa tggtagaaat
     4861 tccactgtta ccagggtctc caatcagcaa cattttattt acccaggcac ctgcgtttcc
     4921 ggatacattg atattctttt ccacagcata aattttagta aaaagcacat ccaattgact
     4981 gatattttct gctgaaattc taccgataaa agcgtctccc aaataatcgt tacctgctaa
     5041 aaaagtataa ggataatccc ctttacctcc ataagtggac atttgttcag tatatgtagg
     5101 aatggcataa cttccgttag tatcacctaa aaggataata aaatcgggac gcgtagaagg
     5161 attatcatat tgcgtttgga tatagttctt aattgcctga gtagaagctc ctccggtttc
     5221 aactgtgctt acaaaattta catcatagcc cttttgcctt ttccaggcaa caaattcatt
     5281 cagtttggca atgaaaatag catccgtatt gtttccgtaa ataagtaata ttcttgcagg
     5341 ttgaggagcc atcatcgtat tccggtaata atcaaaattg gcaatcatag attcataaag
     5401 attggtgaag gcataagaat agccgttgta ttcatccatt tcattgattc cctgtctgtc
     5461 cttaacctct atttcaactt ccatctgttt ttggattttc agttcttgcg tttctgctac
     5521 atattgaacc ggattaagag taatctgcac aaccctgaaa tcacggataa tttggggttg
     5581 actatagctg taactatttg caggatataa ggaagcacta tgataagctg ttttgttata
     5641 ttcgcttgca atttcctgtt cttttgtggc aaaaaccggt ttaggaataa accccttttg
     5701 ggtaataatc tctcctccgg tcacttttat ttctatatct ccttttggag gaatagcaat
     5761 ccaagcagaa aaaacgggca attcaggaaa ccctgtttcc cctgttgttt ctgcaccttg
     5821 catactcagg attttaaagt tgctgttatc aatatcttca atttgcagtt cgggaacctg
     5881 taattgcagt tttatctttc ctgcattctg agaaaccacg gaaaggggtt tttctatcgg
     5941 aataattgtt cctgcaaata aggtactgaa cgaacacatc aggattagaa tggcaagttt
     6001 agttgttttc atctttggta aacctttatt tggtataaat taaagagaac ctgaagttaa
     6061 atttactcta acttcaggtt ctttcccatt gtaaaattgt ctcataaatt actttatctc
     6121 gtagataaga atgccctgat cgcgagctcc tactactaat ttattatcca taaatttcac
     6181 agtattggtg taaccgcagg aagttaaatg ctgtaacaat tgcggttttg aaggaatact
     6241 aacatcaaaa agataaatac ctcctcctcc ggaagaaaga gcaagtttat ttcctgaaac
     6301 agctaaggaa gtagcataac cacttgtatc aaatgttgac agcaacactg gtgaagcagg
     6361 attgcttaca tcaacaatat gtaaaccgct ttggcgtcct gctacataag caatattgtt
     6421 attaacttcc actttttgag cttctccgct taaatgaata ttgccaatca gctgacggtc
     6481 attacgatta taaatatata aaccaagctg ttctgcggct atgaaaatgt aattatcggt
     6541 caatttaaaa ccagcagctg ctccaggaga aatggtaaac acattactgt tgaaaattgt
     6601 gccatcataa taatcatatt tgaaacttcc tgctgcacaa taggcacaaa ccattttata
     6661 agttccctgt aaaaaaggaa caggagttat ggcaaaacta tccaaatcct ttatattact
     6721 tgttccacct ataaaatcaa gaatgtaaat tagggaatcg ggatcgctgg tattaattac
     6781 tctaatagca tcagttgcct cggtatccac taaaaacata aaattatgtt ccggtactac
     6841 cgctaccttt ttaattctgc ctaaaacttt ctggctacca tcttctgccc aaaattcagt
     6901 aatccactgt atatgataat ccgttctact gattcgggac attccaccct ggtcatgtgc
     6961 tacatatata taattatcat caatcttaat atccaaagga ttgcctacaa tcggtaaaac
     7021 atttctcaaa gtaagccaat ctccttcctg atattcagta ttgcgttttg cgcatgaact
     7081 tagcaatatc agcacgatga ttgctaatgt tattatataa cttttaggca ttgagttcct
     7141 ccactgcctt ttttcatctt tcctatagaa taatgcaaaa aacattccga aataaaatat
     7201 gctttacatt atgataggac atataaagag aaatgtcagg tttgtaactt ttttatagat
     7261 tgccaatgaa taaaagtcca tcgctatcat tgtatcgttc ttcggatttt tgtggttgaa
     7321 tgtttcatag tgttgttcag attaaaaagg tattgttagg tatttggcat atattatatc
     7381 attttgaatg ttaagccatt tttcattcct gcgcaagcag aaatcattaa aaacagaaag
     7441 agctctctgc ttcttttcaa gagagctctt ttttaggatt agggttggag atttctatgc
     7501 caacattttt gctatatcgc tgtccgcatc ggtaatttgt tgcaaaccaa aggtatcaga
     7561 aataactttt gccacattgg gagaaagaaa tccgggcaaa gttggaccta aaataatatt
     7621 cttaaatccc aaagaaagta aagctagaag aacagctacg gctttttgtt cataccaggc
     7681 aagatcaaaa gaaagaggaa gtttatttac atcatccaaa ccaaatgcct ctttcagttt
     7741 caaagcaata actgccagcg aataggaatc attgcattgt ccagcatcca aaacccttgg
     7801 gattccatta atatcgccta aaggaagttt tatataacga tatttggcac atccggcagt
     7861 aagaataacc gtatctttag gcaatttttc cgctacttca gtaaaatatt tcctgcttgg
     7921 tattcttcca tcacaaccag ccatcacaac aaagcgtttt atagctccac ttttaactgc
     7981 ttcaatgatt ttatccgcca agcttaaaac agtggaatga gcaaaaccgc cggtaatttc
     8041 accagtttca agttcttttg gaggttggca ttttagagct cttgcaatta cagggctaaa
     8101 atccttttct ctgccttttt ccctatccgg aatatgcact gctccaggat aatttactgt
     8161 tcctgtagta aataaacgct ccaaataggt attttccttg cgcaagggaa caaggcaatt
     8221 cgtagtcatt aaaatagctc cgttaaagtt ttcaaagtcc tccagttgat gccaccagga
     8281 atcaccataa ttaccgataa agtgtttgta tttcttaaaa gcgggataat aatgagcagg
     8341 cagcatttct ccgtgagtgt atatatcaac acccttgcct tcggtttgtt tcagaagttc
     8401 ttccaaatcc ttcaaatcgt gaccgctaat taaaattccg gggttcttac ctactcccag
     8461 ttttactttt gtgggttccg gattgccgta ggttttggta tttgcctcat ccagttttgc
     8521 cataacttta acggcatttt ctcctgtccg caaaaccaaa tttaccagct gatcaacagt
     8581 taaattatca tctaaagtgg cggacagacc ttccataatg aacttgttta catcatcatc
     8641 ctgaaaacct aaaatggcag cgtgttcacc gtaagcgcaa atacctttta tgccgtaaat
     8701 aattgtttct cgtaaagaac ggatgtcctc attttctgtt ctaagcacac caactttttt
     8761 ggcaaattca tcatattcat ctcttttcag ctgaaaagta actgcttcgg gacaggtatc
     8821 acatttacct tccatcaatt tacagagctc atctttccgc ttatccctca cagccaaagt
     8881 tgtttcaatt gtgtctttga tgttttcttc gtcccaattg acattagtaa tagttttaaa
     8941 taaagcgctc atcacaaaaa cggaatcttc aggataatat ctatcctttt cctgcagttt
     9001 cacggataca taagcgagac ccttcaatcc gtaaatcaac agatcaaaca aattggcaag
     9061 tgtttcactt tttccgcaaa caccacgcat tgtgcatccc aggttacgag atgtttcctg
     9121 acattgataa cagaacatac tcattttatt ttctcctttt ttagggtttt ggcattttaa
     9181 ttaccaataa tcttaaattt gaatttcctt cattgaatac accgtgagga atatcctttg
     9241 gactttcaat cagagtgtct tcaggaaaac tctgtttctc ttctccaatt tccaaagtgg
     9301 ctgttccttc caatacatag aaagccacat ttacaggagt gatatgtttc tttaaatgag
     9361 ctcctggtgc taaattaaga tgaactatct ctccttcggg tgcttcgtaa attttggaac
     9421 ccaaaatacc attggcattt actttgggtt ccacatcctg ccaggttctg cttttcattt
     9481 ttcctcctta ttgttttgtt tctattagtt ttattattgt ttctatcagt tctgaacctt
     9541 ctttcttcag atcaaaagcg ccatcactca aattccactg agtaactgta aatctcattg
     9601 aacccacaat aattctgaat aattgagttg gagaaaggtc acttcggatt ttgccttcct
     9661 gttgtgcctg aatgatataa ttttccatta cctgcttgtg tttatgcata atacactgaa
     9721 actgaccttt aaaggtagga tcatttttaa atagctcttc ggaaaacatc acttttgcca
     9781 ggttgggatg ctcactgaaa aggatatacc tatcctccac aaaatgcctg atactttcaa
     9841 gtggagaata atctttttct cttatttcct gtaaaacctt atttgaaagt tcctcaaaat
     9901 aacctaaaat cgttgtcact aaatcccttt tacttttaaa atgtctgtaa agtgctgctt
     9961 ctgtaacccc tattttagtt gccagattct ttgtagttag cttttcataa ccttgattag
    10021 caattatagc aattccagcc attattatat ctttttgtct ttcagttagt tccatccttt
    10081 ttcccttatg taagtaagta ttcactaacc aaaatattca agcccgcata aatgtcaaga
    10141 aaaaagtaag aaaagttgag aagtcaagag atagagagga taccttctat ttgtgtagcc
    10201 ggaggatgtg gttcctacgc tgcaaaaaaa tgctgagcta cagcgaattc tggcaacaca
    10261 tttttccagt ttgtaactta cagaaaaaaa taaatcggct ttggtgtcct cagtaacctc
    10321 attgttctta gtgtaattaa atctgcttga gttatttttt aattgttaat ccaagcatat
    10381 cactttagta tattattaga ggaatatagt atgcgaccgg ataaaaataa actaatagaa
    10441 ctgctttcca ccgaagggga agagaacctt caaacattat ataatcaggc atacaaggtt
    10501 aaaaaaaagt atgtgggcac taccgtatac ctgcgtggtt taattgagct tagtaatatc
    10561 tgttccaaga attgttatta ttgtggaatc cgtagtggca ataaagaact taagcgttat
    10621 cagatcagtt atgaagaggc aattgcagag gcaaaattat gccaacaaat gcattatggt
    10681 tcaattgtta ttcaggcagg ggaaagaaac gataaaccgt ggattgattt catcaccaag
    10741 ctcataatag ggatcaaaga actatctgaa ggtaaattag gaattacttt atcccttgga
    10801 gaacaaaagc gagaggtcta tcagcaatgg tttgaagcag gagcacatcg ttatctttta
    10861 cgcattgaga cctccaatga agagttatac aaatcattac atccagctgc tcaaagtttt
    10921 gaaacacgtt taaaatgctt gcattcaata aaagaatgcg gttatcaagt tggcacagga
    10981 gttatgattg gtttaccggg acaaacaatt gaggatttag ccaatgatat actatttttc
    11041 tatgatgaag atatagatat ggtaggaatg ggaccctata tccctcatca taatacacct
    11101 ttaaagcatt tggtagatag ttttaatcct gcagaggcat taaaaatggg acttaaaatg
    11161 attgctgcgt gtcgcatagc tctaaaggat gttaatattg cttctactac tgctttgcaa
    11221 gcgctacatc cggaaggtag agaattgggg ttaaaagcag gtgccaatgt tttaatgcct
    11281 aatataactg atacaaaata cagaaagggt tatcaacttt acgaggggaa acctggcttg
    11341 aatgaaactg ctattcaata tcaaaaggaa ttggaacaac gcatttctgc ccttggagaa
    11401 acaattggct acgatgaatg gggggattca aaacattttt acagccgaga agtgcagagg
    11461 tcaatgaaga atgaaaaatg aagatagctc cgaaaccata aagaaagctc aatgttctat
    11521 gtgttttcgg tgttcttggt gtaaataaat cttttccttg acggatacac cttattccaa
    11581 gaataagcac aatactataa gaaaaggagt tttatatgct tcccggtgga aaaaatatgc
    11641 agcaattgat gaaacaagct caaaaaatgc aggcagaaat aatgaaacaa caagaagaac
    11701 tggcgaatac agttttttcc gcttcttcgg gtggaggaat ggtaactgtt gaaatgaatg
    11761 gacagtttga agttgtttcc ttaaaaatag accctcaagt tgttgatccg gaagatgtag
    11821 aaatgctgga agaccttatt actgcagcag ttcaggaagt tctaaacaag gtgaatgaag
    11881 catccagcaa tgtaatgagt aaagttacag gcggaattaa tattcccggt ttgggttaaa
    11941 tgcttttttc cgaaaactta gaaaaactaa ttcagagctt taaccgtttt cctggtattg
    12001 gacgcaaaac cgctcaacga cttgcctggt atctggttgc gcaggataaa aactttgctt
    12061 taaacctggc ggaaacaata aaaacaaccg ttgagagttt taaaacctgc agtcaatgta
    12121 atatgctttc agaaagcgat ccctgtcctt tttgcatttc tccagaccgt tccgacagtt
    12181 atctttgcgt ggtagaaaac agttccgaca tccagatatt agaaggtatg aacgaattta
    12241 aaggcaggta ttttgtttta gggcatttgc tttccccttt ggatggctac ggacctgaac
    12301 aaataaacac ggctatgttg ttggagagaa tagaaaatct tcatccaggc gaagttattt
    12361 tagctctaaa accctctgcg gaaggtgaag caacaattca ctatctttgg gaattgctaa
    12421 aagataaagg aatcagcata acccgccttt caaccggaat tcctttcgga ggagacctgg
    12481 aatacagtaa cttaaatact ttagccaatg cctggaaacg cagatatacc gttcagtaat
    12541 atgtttaccg gaattattga agcgacggga aaaatagtta ctcaccagct aataagcgga
    12601 aaaaagctga taacaatttc ccgacccgct atttttgatg acctgaaaat tggttgcagt
    12661 attgcttgtg atggaatttg ccttacagtt gttcagtttg atgacaaggc atttacagtg
    12721 gaaataatga acgagacact ccaaaaaagc acagctaaaa actggacttc aggtaaaatt
    12781 ttaaatctgg aaagagcatt aaaactgaat tctcgtctgg atggacactt tgttcagggt
    12841 cacatagata gagaattgcg cttgatttca catctgaaaa gagaaggaac tgattatcta
    12901 catttggaac ttcccagtca ggaaagaaat ctaattgttc ctcaaggtgc aattgctctg
    12961 aatggagtca gtttaaccat agcggaaatt cgcaataatt attttagtgt tgccttaatt
    13021 ggtcatactt taacgcatac aaatctggga acaataaaaa ctggcgaaaa ggtaaatgtg
    13081 gagtatgata tcttaggcaa gtatgtttta gcgcagatac cgaaaggtta atcaaaaaaa
    13141 gataaaattg ttttacccca aagggttaaa gtttatgaga tttaataaga cctttatctg
    13201 cctcttcttt ctgctgatta tactatttct aatcagcggt tgtggcaata aacgcagtcc
    13261 tacaggcggt cctcaagata ttgataagcc agtagttctg gaaagttctc cggcagaatt
    13321 tggtgatatt tcttccggat atattgagat atctttttct aagccaatgg ataggaatac
    13381 attagccaat tctatttaca tctatcctcc cgtgcagaat aaaaaaatta ccttggatgg
    13441 agccaatctt aaaatccata ttaaggaacc cttaaagccc gataccaatt actttattac
    13501 tttaaccaag cgtcttaagg atttacgtgg caacaccttg gaaaagaatc aaacccttat
    13561 ttacagcaat ggaaaacttg ccaccaatcg tattgctgga acaatcactt atgaagaacc
    13621 aactgataag ggtttgcctt tggagctaac tcttttgtct gccgattctt tattggtttt
    13681 aagcagtgtt attcaaggtg gtgcttatgc cattgacaac ttgaatcctc agaaacatat
    13741 cctccgtact tatatagata aagattttaa tggtcgttac gattttggta gagagccgtt
    13801 ttttgaaggg attaccgatg gttcttccgt ggcaacttta aatataacaa tggcttacgg
    13861 tgatactacg agagctaaaa tatcccaagt gaatgtgctt agtgaccgcg aactgcaagt
    13921 tattttaagt gaagatatta agagctatga tgcacttaac atccagacaa aaaatacccc
    13981 tttatccatt gcctatcaat tgattgaagg atatataatt aatttattaa ccgccaaaat
    14041 ggatagcacg gaatatacct taacccttac tggagtgaaa gataaaaaag ataacatcag
    14101 taacaaactg gaaaccaaat ttaaagttag acccgtagct gatactttgg ctcccaaaat
    14161 catttttaca aatcccagaa acggtgcttc ggtaaatact cttttacctg ttttggaaat
    14221 ccattttaat gaaattattc cgaaagccaa tattaaagcc aaattgctct gcggaaatca
    14281 ggaaatacct ctcaaacatt tgtctgaaac tggacgcatt caccgttttc aacccccaaa
    14341 agaactggaa aattacaaaa gccacctgct aattatcagt tcagaaacca cagattttag
    14401 tggcaatcac cttaaaaatg attatgaatt gcagtttctg cctctcctga gaaaatagat
    14461 atcgggataa tagctctgtg gagaaaattt tgactcacaa ttttcacctg ccttccagaa
    14521 aaacacaatc tacgaattat ctcacttaca actgcatttt aacgggcttc caaagtgaaa
    14581 aattgtgagt taaaatagaa atccattctt aaaaaaataa ataggtagtt tcctttactc
    14641 ttttcgccgg gttaaaaccc gtcgttaata cctgtcgttc ctacggaact cattctaaaa
    14701 aaaagaaata gatagtttcc tttactcttt tcgccgggtt aaaacccttc tataatacct
    14761 gtcgttccta cggaactttt ttcctattat ttgtggttta tgaatataac tattgtataa
    14821 aatatgaagt taccaataca attttgtatt cttctccgga atactcctaa ctcctacaaa
    14881 ataaatataa ttccgttttc ttccttaata gca
//
LOCUS       DAOOZV010000045        14661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4133_length_14661_cov_61.510201, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000045 DAOOZV010000000
VERSION     DAOOZV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_14661_cov_61.510201"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..808)
                     /gene="rpsG"
                     /locus_tag="PLW08_05600"
     CDS             complement(338..808)
                     /gene="rpsG"
                     /locus_tag="PLW08_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPL52115.1"
                     /translation="MPRKHRAVVREVLPDPKYNDITVTKFMNCLMVKGKKSIAEKIVY
                     GAFDIIAAKTKEDPLVVFKSALDNVRPLVKVVSRRVGGATYQIPIEVNEKNGKALAFR
                     WIIANARARSEKTMTEKLAAELIAAHKKEGASIKKREDTHKMAEANKAFAHLRW"
     gene            complement(825..1211)
                     /gene="rpsL"
                     /locus_tag="PLW08_05605"
     CDS             complement(825..1211)
                     /gene="rpsL"
                     /locus_tag="PLW08_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPL52116.1"
                     /translation="MPTINQLVRKGRAEVEKKRKNRALMECPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLVNGFEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DSAGVEKREKSRSKYGTKRPKAKTVK"
     gene            complement(1254..5735)
                     /gene="rpoC"
                     /locus_tag="PLW08_05610"
     CDS             complement(1254..5735)
                     /gene="rpoC"
                     /locus_tag="PLW08_05610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPL52117.1"
                     /translation="MLKESKHEIKPNEYDAVRIKIASPETIRNDWSHGEVTKPDTLNY
                     RTLKPEKDGLFCERIFGPERDYECACGKYKKKRFQGTVCDRCGVLVTTSRVRRTRMGH
                     IDLAVPIAHIWFVKSAPSKIGTLLDMTVKDLERVLYYESFIVIDPGDSPFEKMELLEV
                     DEYYEIKDKVADNFLALMGGEAIRELLKRINLKNEALDLRTRLKFEESALRKQKLINK
                     LKIVDAFIKSGNRPEDMILEALPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNR
                     LKALLEIRAPEVILRNEKRMLQEAVDALIDNGRKARPVRGRGNRPLKSLTDQLKGKQG
                     RFRQNLLGKRVDYSGRSVITVGPELKLYQCGLPKEMAVELFKPYLIDKLQKIGEVDKV
                     KNAKKLIEKKQPEIWSILEEVIKDYPVLLNRAPTLHRLGIQAFMPVLTDNKAIQLHPM
                     VCVPFNADFDGDQMGVYIPLSVESQIEARVLMLSTRNLLLPANGRLAMAANQDIVLGC
                     YYLTMEETPPPVEIEKLRHFYGPDEVIAAYEAEEQFCSSKGETVYERNLDLHTWIRLK
                     LESEYIVTTVGRVILNQIMPPEVGFQNIVFDKGKLNDLAMLCFDAVGQWRTAQILDQI
                     KELGFHYATRAGVTFSFDDIVVPKRKDEIIAQADQEVKKITDLHQKGGITENERFGRV
                     VDQWKKTTVRVTDEMMEELSKTRNGLNSIYMMYKSGARGSKDQIKQLGGMRGLMDKPT
                     KIGSTGGADVIETPIKSNFKEGLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLVD
                     VAQNAIITIEDCGTTRGVNMTALKEGNEIVQTLAERIQGRTAVDDIISPITGRILVSA
                     GEEISNKMARTIQNHGLISVHVRSILTCEAEKGICAKCYGRNLATQKPATIGDPVGII
                     AAQSIGEPGTQLTLRTFHIGGAASTATELAEVVASHDGIVKFDRMNTVINTENQLISV
                     SYLGKILIVDENDENKVLEEFKVEYAATVHVQDGQKVAKDTKLLSWDQFNNPLISTAK
                     GVLHYENFIKDITYKEEYNDITMARELTIIESKDRRKQPQFRIVGEDGTVRLIPLPTG
                     LIVRVEDGSFVHIGDILGQTSRMTIKQRDITGGLPRVQDLFEARVPKDKAKISDIDGI
                     VTIGGLKKTGRDIFVTPPNGLFAPCDGKVVVLYDEDKNQYIAVLPDKAVYSENDGKVS
                     IIEENRKKIIQVAKRNQNPVQYPVPKGLQILVKEGESVKKGTPLCGKLYAVPPEQESI
                     VETGDNVTMHQPLAGRKYIIPSGKRIIVHQGDYVESGDALSDGPLDPHDMLVKGVIEA
                     QILILNEIQEIYRKQGVKIDDKHVSVIIRQMFKKVRITDSGSTSFLQGDIVDKVLVEK
                     ENREAIEFGKTPAQFEQLLLGITKTSLLTDSWLSAASFQETTKVLTKAAIEGRVDHLE
                     GLKESIIIGHRIPVGTGTKLYNNMLKKAVNEGKTVAQIIDEFAHRSKEEESGDYLDF"
     gene            complement(5749..9528)
                     /gene="rpoB"
                     /locus_tag="PLW08_05615"
     CDS             complement(5749..9528)
                     /gene="rpoB"
                     /locus_tag="PLW08_05615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPL52118.1"
                     /translation="MRKVKSYSRISGRFAELGIPEVEIPNLLAMQVDSFNDFLQKDIH
                     PQRREKKGLQAVFENIFPLEDSKGNFLLEFIEYNVLQEKYSLEECRERNLSYQAPLKA
                     KMRLSVFENREGVREHKDTLEQEVFLGEIPLITEQGTFVINGAERVIISQLQRSPGVF
                     FSEEKHPSGKTLYSAKVIPYSGSWLDFDIDIHDVMFVHIDKRRKLPVTTLFRAIGIST
                     NEDLRKTFYKSEKLNLKEAKGRHLFADIYEKGNPEPIAMANEQITDSLIKFLSERKIT
                     KVEVIDYNYEIARKVLENTMAKDPTTNQEEALKKIYNLIRPGEDAPLEAAKQLVDRMF
                     FNEKRYNLGEVGRYKINSRLGINVDPKIMTLTVEDFVYIFKTLINIYHDEDEVDDIDN
                     LANRRVRTVGELLQEQYSIGMAMVARIIQERMAISNIDEVTVHDLVNSNALISVVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLARERAGFEVRDVHSSHYGRICPIET
                     PEGPNIGLIVSPAIYSRINHFGFLETPYRKVVDGCITDEYEYLDASQEEKYIIAQSDV
                     HLDDNRRITDEMIFARDRGEFVQVSPNLVHYMDVAPQQMVSVSAALIPFLEHDDANRA
                     LMGSNMQRQAVPLINPQAPLVGTGMEKIASMDTSSIAVAPYDGIVTKVTSAFIDLKPI
                     NEKDEAYYLSTGNRIHLKKFVRTNQDTCINQRPIVRVGDTVKKGQPISDGACVEDNRL
                     ALGTNLMVAFMPWYGYNYEDAVILNEKVASEDTLTSIYIEELEVLVRNVKNGREELAY
                     DIPNVPAQALRNLDKTGIIRVGSVIHAGDIIVGKVTPKSVEIDPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRLEEGSDLEEENEDKIEKLKEELALRRSKIEE
                     FKEEKLSAVLVGQIAKNIWDEKTNIPFIAPGKKITKQDLKRINFKKLDLDVELVEDRE
                     VNDYIYSQIVLKIKQSLEQSDNIYKKSRERIKHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIIAPIEDMPFMEDGTPVDIVLNPLGVPSRMNIGQIMETHLGWAA
                     KTLGFEVETPIFDGASNEDIVSELRKAGLPEDGKTVLYDGKSGEPFKERVTVGIIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGAANVLEEML
                     TIKSDDVDGRNNAFKAITRGENPPPPGVPESFNVLISELKSLGFDIEFIKEEENK"
     gene            complement(9737..10120)
                     /gene="rplL"
                     /locus_tag="PLW08_05620"
     CDS             complement(9737..10120)
                     /gene="rplL"
                     /locus_tag="PLW08_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPL52119.1"
                     /translation="MSDKKQQVIDLIKEMTVLELSELVKEMEEIFGVSASAPVAAFMP
                     TQGSAAEGGEKEEQTEFDVILTSAGDKKINVIKVVREITKLGLKEAKDLVDGAPKVVS
                     EKVSKEEAESIRKKLEEAGATVEVK"
     gene            complement(10153..10689)
                     /gene="rplJ"
                     /locus_tag="PLW08_05625"
     CDS             complement(10153..10689)
                     /gene="rplJ"
                     /locus_tag="PLW08_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPL52120.1"
                     /translation="MVQSIKYKIVKDLTERLSGARAIVLVDYKGINIEQVNQLRNRFR
                     ESQVDYFVQKNTLIKIALNDLGITELDPYLKGPTAVAVSKLDEVSPARELIKFLKEVM
                     EDKGFPSFKVGYIAGHIFNADELTALAKLPSREELLAKVLAGMNAPLSNFVSINQGIL
                     RKFVYALDAIAKKQAEAS"
     gene            complement(10702..11406)
                     /gene="rplA"
                     /locus_tag="PLW08_05630"
     CDS             complement(10702..11406)
                     /gene="rplA"
                     /locus_tag="PLW08_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPL52121.1"
                     /translation="MKHSKRYNVAYTMFDRQKRFDLNEAIKLLKSLPTSKFDETVELH
                     FNLGVDPRKADQQIRNSLVLPHGTGKEMRVLVFAEGDKAEEAKKAGADYVGVDDLIEK
                     ISNGWFDFDVVIATPNLMGRIGKLGRVLGPRGLMPNPKVGTVTMDVGKAVQEAKSGKI
                     TYRVDKFANLHIPVGKLSFEEEKLKDNIKAVLAAVLRDRPPTLKGVYIKSITLCTTMS
                     PGIKIQVASATAEARS"
     gene            complement(11448..11879)
                     /gene="rplK"
                     /locus_tag="PLW08_05635"
     CDS             complement(11448..11879)
                     /gene="rplK"
                     /locus_tag="PLW08_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPL52122.1"
                     /translation="MAKPKDAVATLKLQLPAGKATPAPPVGPALGQVGVNIPEFCRQF
                     NEKTADQPGMVFPVIIYVAKNKSFTFEIKTPPAAVLIKKEAGLATGSATPNKLKVGKI
                     TNEQLKNIAQLKIKDMNCNSLESAMSMIAGTARSMGVTIED"
     gene            complement(11904..12437)
                     /gene="nusG"
                     /locus_tag="PLW08_05640"
     CDS             complement(11904..12437)
                     /gene="nusG"
                     /locus_tag="PLW08_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPL52123.1"
                     /translation="MKWYVIHTYSGHENKVKTAIEKGLAGTPLEKQVGRLLVPVRKTF
                     VIREGKKIERDKKLFTSYVIMEADMTPELKTYILQIAGVTSFLGINKEHKEPIPLSQE
                     EVDRLLGITDPNRDIKVYSYMPGDMVKVISGPFTDFEGIVQKTTENGTKLIIEVTVFG
                     RRTPVELNASQVELIKK"
     gene            complement(12455..12640)
                     /gene="secE"
                     /locus_tag="PLW08_05645"
     CDS             complement(12455..12640)
                     /gene="secE"
                     /locus_tag="PLW08_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPL52124.1"
                     /translation="MKFEKITRFFRDVRSEMKCVSWPTKTDLKEGTLVVIIMSAIVAI
                     FLSLVDFGFTKIVELIF"
     gene            complement(12708..12783)
                     /locus_tag="PLW08_05650"
     tRNA            complement(12708..12783)
                     /locus_tag="PLW08_05650"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12748..12750),aa:Trp,seq:cca)
     gene            complement(12809..12955)
                     /gene="rpmG"
                     /locus_tag="PLW08_05655"
     CDS             complement(12809..12955)
                     /gene="rpmG"
                     /locus_tag="PLW08_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPL52125.1"
                     /translation="MRDIIILACEECKNRNYTTTRNKRKHPTRLELKKYCPTCRKHTV
                     HKQR"
     gene            complement(12975..14159)
                     /gene="tuf"
                     /locus_tag="PLW08_05660"
     CDS             complement(12975..14159)
                     /gene="tuf"
                     /locus_tag="PLW08_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPL52126.1"
                     /translation="MAKEKYVRTKPHLNVGTIGHIDHGKTTLTAAITLYLSKKGGAKF
                     RTFDSIDNAPEEKARGITIATAHVEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSADDGPMPQTREHILLARQVGVPAIVVFMNKCDLVDDPELLDLVEMEVRELLDK
                     YEFPGDEVPVIRGSALKALNGDPEAEKQIQALLDAVDSYIPLPERPIDKPFLMPVEDV
                     FSIPGRGTVATGRVERGVIKVGDKVERVGIRETVETTCTGVEMFRKLLDEAQAGDNIG
                     VLLRGFGKKDVVRGMVLAKPKSITPHTKFIGQTYVLTEEEGGRHKPFQSGYRPQFYFR
                     TTDVTGSLYLPEGVKMVMPGDNVEIQAELITPIAMEQGLRFAIREGGRTIGSGVVSKI
                     IE"
     gene            complement(14292..14367)
                     /locus_tag="PLW08_05665"
     tRNA            complement(14292..14367)
                     /locus_tag="PLW08_05665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14332..14334),aa:Thr,seq:ggt)
     gene            complement(14384..14460)
                     /locus_tag="PLW08_05670"
     tRNA            complement(14384..14460)
                     /locus_tag="PLW08_05670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14424..14426),aa:Gly,seq:tcc)
     gene            complement(14486..14571)
                     /locus_tag="PLW08_05675"
     tRNA            complement(14486..14571)
                     /locus_tag="PLW08_05675"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14535..14537),aa:Tyr,seq:gta)
     gene            complement(14586..14660)
                     /locus_tag="PLW08_05680"
     tRNA            complement(14586..14660)
                     /locus_tag="PLW08_05680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14626..14628),aa:Thr,seq:tgt)
BASE COUNT         4016 a         3264 c         2597 g         4784 t
ORIGIN      
        1 gaatactcct aactcctaca aaataaatat aattccgttt tcttccttaa tagcattgct
       61 tacccattgc ttacccatta ctaatggcat tagcaatgag tccgtattga gtaagcaatg
      121 ctacttggga caataactta caaaagtgta aacgaaggat gccgttcctc cgcaggaata
      181 atggttaaaa aaaagtattt gtagataact ccgtaatggg caaaaaaata ttttgttaca
      241 gcagaattag aagcggataa aggtaataaa agaaaatgcc ttcactctca tcagatgagt
      301 aaaggcatta aatattctga tatcttaaaa aaagagctta ccatcttaaa tgggcaaaag
      361 ctttattggc ttcagccatt ttatgggtat cctctctttt ctttatggaa gcgccttctt
      421 ttttatgagc tgcaataagt tcggcggcaa gtttttcggt cattgtcttt tcgcttcttg
      481 cgcgtgcgtt ggcaataatc cagcggaaag ctaatgcttt accgtttttt tcatttacct
      541 caataggaat ttgataggtt gctcctccca cgcgtcggga aacaacttta actaaaggac
      601 gaacattatc caaggcggat ttgaagacaa caagggggtc ttctttcgtt ttagcagcga
      661 ttatatcaaa agcaccatac acaatttttt cggcgatgct ttttttgcct ttaaccatca
      721 ggcagttcat aaatttggta acggtaatat cgttgtattt aggatccgga aggacttccc
      781 ggacaacagc tctatgtttt ctgggcataa gttctatcct ccctttactt tacggttttt
      841 gctttaggtc gtttggttcc atatttggaa cgggactttt cgcgtttttc tactccagcg
      901 gaatcaagag ctccgcgaac gatatgataa cgaacacccg gaaggtcttt cacgcgtcct
      961 ccgcgcacta aaacaataga gtgttcctgt aagttatgac cttcaccggg aatatatgct
     1021 gtaacttcaa atccatttac aagacggaca cgagctactt ttctgagagc tgaattaggt
     1081 tttttgggcg ttgttgtata aacacgagta caaactccgc gcctttgcgg acattccata
     1141 agcgccctgt tttttctttt cttttcaact tcagctcttc ctttacggac taattggtta
     1201 atagttggca ctgattttct ccttttctat atttttagtg tttaagatta tttttagaaa
     1261 tcaaggtagt ccccgctttc ttcttctttt gaacgatgtg caaactcgtc aataatctga
     1321 gcaacagttt taccttcgtt aactgccttt ttaagcatat tgttatagag tttggttcca
     1381 gttccgacag gtattctgtg accgataata atgctttctt tcaagccctc aagatgatca
     1441 accctgccct caatagctgc cttggtaagc acttttgtgg tttcctggaa ggaagctgca
     1501 gaaagccagc tatcggttaa cagagatgtt ttggtaattc ctaaaagcaa ttgttcaaac
     1561 tgggcaggtg ttttgccaaa ttcaattgct tcgcggtttt ctttttccac cagaacttta
     1621 tcaacaatat caccctgcag gaaactggtg ctgccgctat ctgtaattct aactttttta
     1681 aacatttggc gaataataac gctcacatgt ttgtcgtcaa ttttcacacc ctgcttgcgg
     1741 taaatttcct gaatttcgtt taaaatcagg atttgagctt caataacccc tttcactaac
     1801 atatcatgtg ggtcaagagg accgtcagaa agtgcatctc cactttccac atagtcaccc
     1861 tgatgaacta taatccgctt tccggaagga attatatact ttctgcctgc aagaggttga
     1921 tgcatagtaa cattatcacc tgtttccaca atactttcct gctctggagg aacggcgtaa
     1981 agtttgccgc aaagaggagt tcctttttta actgattctc cctctttaac cagaatttgc
     2041 aagcccttag ggacaggata ttgaaccggg ttttgattgc gtttggcaac ctgaataatc
     2101 ttcttccggt tttcttcaat aatacttacc ttgccatcgt tttcactata aacggctttg
     2161 tcgggaagga cagcaatata ttgattttta tcttcatcat aaaggactac gacctttcca
     2221 tcacaaggtg cgaaaagtcc gttaggtggg gtaacaaaaa tatctcttcc ggttttcttt
     2281 aagccaccga tagtaacaat tccgtcaata tcggaaattt ttgctttatc tttgggaact
     2341 cttgcttcaa aaaggtcttg cacacgaggt aaaccacctg taatatcgcg ttgtttaatt
     2401 gtcattcgtg atgtctgacc taaaatatca ccaatatgga caaagctgcc atcttccacg
     2461 cggacaataa gtcccgttgg tagaggtatt aaacgcactg ttccgtcttc acctacaatt
     2521 ctgaattgag gttgttttct ccggtctttg gattcaatga tggtaagctc gcgagccata
     2581 gtgatgtcgt tatattcttc tttataggta atatctttga tgaaattctc ataatgcaaa
     2641 acacctttgg ctgtagagat aagaggatta ttgaactgat cccaggaaag aagttttgta
     2701 tctttagcta ccttttgacc atcctgaaca tgaacggtag ctgcgtattc tactttaaac
     2761 tcttccaaaa ccttattctc atcattttca tccacaatta agattttacc caaataactc
     2821 acggaaataa gctgattttc cgtattgata acggtattca tacgatcaaa ttttacaatt
     2881 ccatcatgcg aagcaacaac ttctgccagt tcggttgctg tagaagcagc acctccaata
     2941 tggaaagttc taagcgttaa ttgtgttccc ggttcaccaa tactttgagc tgcaataatt
     3001 ccaacaggat caccaatagt tgcaggtttc tgagtagcaa gattgcgacc gtaacatttg
     3061 gcacaaattc ctttttctgc ttcacaggta agaatgctgc gcacatggac agaaatcaaa
     3121 ccgtgatttt gaatggttct tgccattttg ttactgattt cttcacccgc ggaaactaat
     3181 attctacctg tgataggact tatgatatca tcaactgccg ttcttccctg aatgcgttct
     3241 gccagggttt gcacaatttc atttccttct ttaagggctg tcatattcac accccttgtg
     3301 gttccgcaat cctcaattgt gataatagca ttttgtgcca catcaaccaa acggcgtgta
     3361 agatatcctg catccgctgt tttaagtgct gtatcagcta atcctttacg agctccgtga
     3421 gtggaaacaa aatattcaag aacagtaagc ccttccttaa aattactttt aattggggtt
     3481 tcaataacat ctgctccacc cgttgaacca attttagttg gcttatccat taaaccgcgc
     3541 attccaccca attgtttaat ctggtctttg ctgccgcggg cacctgattt atacatcata
     3601 taaatagaat ttaagccatt tctggtttta gaaagctctt ccatcatttc atcagtaacg
     3661 cgaacagttg ttttcttcca ttgatccaca actcgtccga agcgttcatt ttccgtaatt
     3721 ccgcctttct ggtgaagatc ggttattttt ttcacttcct gatcggcttg ggcaataatt
     3781 tcatctttcc gtttaggaac tacaatatca tcaaagctga aagtaacacc tgcacgagtg
     3841 gcataatgaa atcccaattc tttaatctga tccagaattt gagctgttct ccattgtccc
     3901 actgcatcaa aacaaagcat agccaaatca ttcaatttcc ccttgtcaaa tacgatgttt
     3961 tgaaaaccga cttccggagg cattatctgg ttgagaataa cccttcctac agttgtaaca
     4021 atatattcgg attccaattt cagacgaatc caggtatgca ggtctaaatt gcgttcataa
     4081 actgtttcac ctttggagct gcaaaattgt tcttctgctt cgtaagcggc aataacttca
     4141 tcaggaccgt aaaaatgtct cagtttttct atttccacag gtggcggtgt ttcttccata
     4201 gttaaataat aacagcccaa aacgatatcc tgattagccg ccatagccaa acgtccattt
     4261 gccggtaaca ataaatttcg ggtagaaagc attaggactc ttgcttcaat ctgtgattct
     4321 acagacaggg gaatataaac acccatttgg tcaccatcaa aatcggcatt aaaaggaaca
     4381 cagaccatag gatgaagctg aattgctttg ttatccgtta aaacaggcat aaatgcctga
     4441 attcccaaac ggtgaagggt tggagctcta ttcaacaaaa caggataatc tttaataact
     4501 tcttccaaaa ttgaccatat ttcaggttgc tttttctcaa taagcttctt ggcattttta
     4561 accttatcca cttctccaat tttctgcagt ttatcaataa gatatggctt aaaaagctcc
     4621 acagccattt ctttgggaag tccgcattga taaagtttaa gttcaggacc cactgtaatc
     4681 acgctccgtc cggaataatc cactcgttta cctaaaaggt tttgacgaaa gcgtccctgc
     4741 tttcctttaa gctgatcggt taatgacttt aagggcctat tacctcttcc tcttacagga
     4801 cgagcttttc taccattatc aatgagggca tcaactgctt cctgcaacat tcttttttca
     4861 ttacgaagga taacttctgg agcacgaatt tccagtaatg ccttcagacg gttgtttctg
     4921 gtgataactc ttctgtataa atcattaaaa tctgcagtgg caaaacgccc tccttccaaa
     4981 ggaactaaag gtcttaaagt tggaggtaaa acaggtaatg cttccaaaat catatcttcg
     5041 ggacgattgc cgcttttgat gaaggcatct actattttca atttattgat tagcttctgt
     5101 ttacgcaaag ccgattcttc aaatttaagc cgggttctga ggtcaagtgc ttcgtttttc
     5161 aggttgatac gcttaagcaa ttcgcggatt gcttctcctc ccataagagc caggaagtta
     5221 tccgctacct tatctttaat ctcataatat tcatcaactt ccagaagctc cattttctca
     5281 aaaggactat ctccggggtc aattacaatg aaggactcgt agtaaagcac tctttccagg
     5341 tctttaaccg tcatatcaag taaagtgcca attttactcg gggcactttt aacaaaccag
     5401 atatgagcaa tcggaactgc caaatctatg tgtcccatac gcgttctgcg aaccctggaa
     5461 gtagttacaa gcactccaca tctatcacaa acagttccct gaaatctttt ctttttatat
     5521 tttccgcaag cgcattcata atccctttca ggaccgaaaa ttctttcaca aaataaacca
     5581 tctttctcag gtttgagagt gcggtaattt aatgtatcgg gttttgtaac ttctccatgc
     5641 gaccaatcat tcctgatagt ttcgggagag gcaattttga tgcggactgc atcatattca
     5701 ttaggtttga tttcgtgttt actttctttt aacattttct ctccccttct acttgttctc
     5761 ttcttctttg ataaattcaa tatcaaaacc cagcgatttc agctcgctga taagaacatt
     5821 aaaggattca ggaactcctg gaggaggtgg attctcacca cgagtgatag ctttaaaagc
     5881 attgttgcgt ccatccacat cgtcactttt gatagttaac atctcttcca aaacgttggc
     5941 agcaccgtat gcttccaaag cccaaacttc catttctcct aaacgctgac ctccatgttg
     6001 agcttttcca cctaagggtt gttgggtaat aagagaatag ggtcctgttg agcgcgcgtg
     6061 cattttatct gctacaagat gattcagttt catcatatag ataatgccaa ccgttactct
     6121 ttctttaaag ggttcacccg atttaccatc atagagaact gttttcccat cttccggtaa
     6181 acctgctttg cgtaattcag aaacaatatc ctcgttagaa gcgccatcaa aaataggggt
     6241 ttctacttca aagccaagtg tcttggcagc ccaacccaaa tgggtctcca ttatttggcc
     6301 aatattcatt cgggaaggaa ctcccaaagg atttaacaca atgtccacag gggttccatc
     6361 ttccataaaa ggcatatctt caataggagc gatgatagaa ataacacctt tatttccatg
     6421 acgacctgcc attttatcgc ctacttcaat cttgcgtttt ttggcaacat acactttaac
     6481 cattttccgc acgccatatt gaagttcatc accatgctta attctttcgc gggatttttt
     6541 atagatatta tcgctttgtt caagtgattg ttttattttc aggacaattt gggaatagat
     6601 atagtcgttt acttccctat cttcaaccag ttccacatcc agatcaagct ttttgaagtt
     6661 tatccgtttc aaatcctgtt tcgttatctt cttcccgggt gctataaagg gaatattggt
     6721 tttttcatcc caaatatttt tagcaatttg tcccactaat actgcagaaa gtttttcttc
     6781 cttaaattct tcaatcttac tgcgtcgcag ggctaattct tcttttagtt tttctatctt
     6841 atcttcattt tcttcttcca aatcgctgcc ttcttccaaa cgggaaaaga cctttacatc
     6901 aataactaca ccctccattc caggtttggc ttttaaggaa ctattggtaa aatcaccggc
     6961 acggtctcca aatagagcac gcatcaggtt ttcttccggt gaaggatcaa tttctacact
     7021 cttgggagta actttaccta caattatatc tccagcatga ataacacttc ctactctgat
     7081 tatgcctgtt ttatccaaat ttctcaatgc ctgagcagga acattgggaa tatcataagc
     7141 aagctcttca cgcccgtttt tcacattacg aaccaaaact tccaattctt caatataaat
     7201 agatgttaaa gtatcttcac tggctacctt ttcgttaaga ataacagcat cttcataatt
     7261 gtaaccatac cagggcataa atgcaaccat taaattagtt cctaatgcca aacgattatc
     7321 ttccacacaa gcaccgtcag aaattggctg cccttttttt acggtatccc caacacgcac
     7381 aataggacgc tgattgatac aagtatcctg attagtgcgc acaaattttt ttagatggat
     7441 gcgattaccg gtagaaagat aatacgcctc atccttttca tttataggtt tcaggtctat
     7501 aaaggcagaa gtaactttcg taactatacc atcataagga gctactgcaa tggaagaagt
     7561 gtccattgaa gcaatctttt ccattcctgt tcctactaaa ggtgcttgag gattaattaa
     7621 aggaactgct tgtctctgca tattggaacc cattaaagca cgattggcat catcatgttc
     7681 caggaaagga attaatgctg ctgatactga aaccatctgt tgaggtgcta catccatata
     7741 atgaacaagg tttgggctaa cttgaacaaa ttcaccccgg tcgcgggcaa aaatcatttc
     7801 atctgtaatt ctacggttgt catccaaatg gacatcactt tgagcaataa tatatttctc
     7861 ttcctgtgaa gcatcaagat attcatactc atcagttatg catccatcaa caaccttgcg
     7921 gtagggtgtt tctaaaaaac cgaaatgatt tatacgggaa tatattgccg gagatacaat
     7981 taaaccgatg tttggtcctt caggtgtttc aatcggacaa attctaccat agtgagaaga
     8041 atgaacatca cgaacttcaa aaccagctct ttcgcgagcc aaacctccag gtcctaaagc
     8101 agataaagct ctcttatgtc ttaaagctgc caaaggattt gtctgctcca taaattggga
     8161 taactgacct gttaaaaaga atgactgaac tactgaaata agagcattgc tgtttaccag
     8221 atcgtgcact gtaacttcat caatattgga aattgccatc cgttcctgaa ttattctggc
     8281 aaccatagcc attccaatag aatattgttc ctggagcaat tcaccaacag ttctaactct
     8341 gcggtttgcc agattatcta tatcatcaac ctcatcttca tcatggtaga tattgatcag
     8401 ggtcttgaaa atatatacaa aatcctcaac agtgagggtc attattttag gatcaacatt
     8461 gatacctaaa cggctattga ttttgtagcg gcctacttct cccaggttat aacgtttttc
     8521 attgaaaaac attctatcca caagctgttt ggccgcttcc agaggggcat cttctccggg
     8581 acgaattaag ttgtaaattt tctttagagc ttcttcctga ttggttgtag ggtcttttgc
     8641 catagtgttt tccagcactt ttctggcaat ttcataattg taatcaatta cttcaacctt
     8701 agtaatcttc ctttccgata aaaacttaat taaagaatcc gttatctgct cgttagccat
     8761 agctataggt tcaggattgc ctttttcata aatatcggcg aaaagatgac gaccttttgc
     8821 ctcttttaaa ttcaattttt cggatttata gaatgtcttc ctcaaatctt cgttagtgga
     8881 aattccaatt gcccggaaaa gagttgttac aggaagttta cgccgtttat ctatatggac
     8941 gaacattaca tcatgaatat caatatcaaa atccagccaa gaaccgctgt aaggaattac
     9001 ttttgcagaa tagagtgttt ttccactggg atgtttctcc tccgaaaaga aaacaccggg
     9061 tgaacgttgt agttgtgaaa taattacccg ttctgctcca ttaataacaa atgtcccctg
     9121 ttcagttatc agaggtattt ctcctaggaa aacttcctgt tccaaagtgt ctttatgctc
     9181 gcggacacct tctctgtttt caaacacgct taagcgcatt tttgctttta acggtgcctg
     9241 ataagataaa tttcgttcgc ggcattcttc cagagaatat ttctcctgca acacattgta
     9301 ttctatgaat tccaacagga aattaccctt tgaatcttca aggggaaata tgttttcaaa
     9361 tactgcctgc agaccttttt tttctctgcg ttgtgggtga atgtcctttt gtaaaaaatc
     9421 attgaaggaa tccacctgca tagccagaag attgggtatt tccacttcag ggatacccaa
     9481 ttcagcaaat cttccggaaa ttcgggaata acttttgacc tttctcaaaa gcgctctcct
     9541 tttggttctt ataaaccaga atgttggctc ttaagtgtta attttttctt tttcacgcta
     9601 ttaggcgaca tttagaatga taaactgact aattggcttt ttcaaaacca aagtttaagg
     9661 atggttgtat ccagaaatta gtccaaaaaa agtccctgcg gacttttttt ggacctttat
     9721 cctgataagg atttggttat ttgacttcga cagttgcacc ggcttcttcg agttttttcc
     9781 taattgattc tgcttcttct ttgctaactt tttcggagac cactttggga gctccatcaa
     9841 caaggtcctt ggcttctttc aaaccaagct tggttatctc tcgcactact ttgattacat
     9901 taattttctt gtctccggca ctggttaaaa ttacatcaaa ttctgtctgt tcctcttttt
     9961 ctccaccttc agcagcagag ccctgtgttg gcataaaagc ggctactgga gcagaagcgg
    10021 atacaccaaa tatttcttcc atttctttca ctaattcaga gagctcaaga accgtcatct
    10081 ccttaatcag atcaattact tgttgctttt tatcggacat tgttcctcca ttggatatat
    10141 aaataatttt tcttagctgg cttcagcttg ttttttagct atggcatcaa gagcataaac
    10201 aaatttgcgc aagataccct ggttgatgct tacaaagtta ctcagcggtg cattcatacc
    10261 ggcaagtact ttagcaagca attcttcacg ggatggtagt tttgccaaag cagtcagttc
    10321 atcggcatta aaaatatgcc ctgctatata accgacttta aacgaaggaa aacctttatc
    10381 ttccatcacc tcttttaaga atttgattag ttcgcgggca ggggacacct catccaattt
    10441 actcactgcc acagctgtcg ggcccttaag ataagggtcc agttccgtaa tacccaagtc
    10501 atttaaggca attttaatca gggtattctt ttggacaaag taatccactt gactttctct
    10561 gaagcggttg cgcaattgat tcacttgttc aatatttatt cccttgtaat ctaccaatac
    10621 gatcgctctt gcgccactta atctttctgt caagtccttt actatcttat atttaatact
    10681 ttgcaccatt ttcctgctcc cttaacttct tgcttccgcg gttgcacttg cgacctgaat
    10741 tttaattccg ggactcatag tcgtgcagag ggtaatgctt ttaatgtaaa cacccttaag
    10801 tgtagggggt ctgtctctaa gaacagcggc aagaacagct ttgatgttgt ctttcagttt
    10861 ttcttcctca aagcttaatt ttcctaccgg aatatgcaag ttggcaaatt tatctacgcg
    10921 gtaggttatt tttccgcttt ttgcttcctg cacagctttt ccaacatcca tagtaactgt
    10981 tcccactttg gggtttggca ttaatcctct gggaccgaga actcgtccca gtttaccgat
    11041 tctacccatt aaattgggag tagcgatcac aacatcaaaa tcaaaccatc cgttagatat
    11101 tttttcaatg agatcatcta cgccaacata atcggctcct gcttttttgg cttcttctgc
    11161 cttgtcgcct tcagcaaata ctaaaactcg catctcttta cctgttccat gaggtaaaac
    11221 aagtgagttg cgaatttgtt gatcagcttt acgaggatct acacccagat tgaaatgcag
    11281 ttcaacggtt tcgtcaaact tggaagttgg taaacttttc aaaagtttga ttgcttcgtt
    11341 aagatcaaat cttttctggc ggtcaaacat cgtataggca acattatacc ttttgctatg
    11401 tttcattgac tctccttatg aatactatct cctgcataag cgaagtatca gtcctctatc
    11461 gtaacaccca tacttcgtgc agttccagcg atcatactca tagcagattc aagggaatta
    11521 caattcatat cttttatttt cagctgagct atgtttttta actgctcatt tgtaattttg
    11581 ccaactttaa gcttattggg agtggcactt cccgtagcta aaccagcttc ttttttaatc
    11641 agtactgcag ctggaggggt tttaatttca aaggtaaaag atttattctt tgccacataa
    11701 attattacag ggaataccat tcctggctga tcagctgttt tttcattaaa ctgacggcaa
    11761 aattcaggaa tattaactcc tacctgacct aaagcaggtc ccacaggagg tgcgggagtt
    11821 gcttttcccg ctggaagttg cagttttaat gttgctacgg catctttagg tttagccatt
    11881 atcttacctc tattgtatct tctttatttt ttaattaatt ctacctggct ggcatttaat
    11941 tcaaccgggg ttctgcgtcc gaacacagtt acttcaataa ttagtttggt tccgttttcc
    12001 gttgtttttt gtacgatacc ctcaaaatcg gtaaaaggac cggaaataac tttaaccata
    12061 tctccaggca tatatgaata gacctttata tcgcggttgg gatcggttat tcccaaaagt
    12121 cggtctactt cttcctgaga caaaggtatg ggctctttat gttctttatt tattcctaaa
    12181 aaactggtta ctcctgcaat ttgaagaata taagttttta gttccggcgt catatctgct
    12241 tccataatca cataagaagt aaagagtttc ttatctcgct ctatcttttt accttctcta
    12301 atcacaaagg tcttgcgcac aggaaccagt aaacgaccta cctgtttttc taaaggagtg
    12361 cccgccagac ccttttctat agccgtctta accttatttt cgtgacctga ataggtatga
    12421 attacatacc acttcatctg cttgtaccta tcccttagaa aatcaattct acaatcttgg
    12481 taaaaccaaa atcaaccagg gacaaaaata tggctacaat agcagacata ataatcacta
    12541 ctaatgtacc ttctttcaaa tccgtcttgg taggccagct tacacatttc atttcggaac
    12601 ggacatcccg aaagaaacga gttatttttt caaatttcat aacattactt ccctttgttt
    12661 gcggaaagaa aaacctgctt cggaaatcat aataccccaa aatgttgtgg caggacaggc
    12721 aggaatcgaa cccgcaaccc ccggttttgg agaccggtgc tctaccagtt gaactactgt
    12781 cctgtgcaac attttttagg gtaaattact aacgctgttt atgaacagta tgtttccggc
    12841 aggtggggca atattttttt agctccagtc gtgtcggatg ctttctttta ttacgggtag
    12901 ttgtataatt acgattcttg cattcttcac aggcaaggat aattatatct ctcattattg
    12961 tttgtccgct tctcttattc tattatttta gagacaacac cactgccgat ggttcttcca
    13021 ccttcgcgga tagcgaaacg cagaccttgt tccatagcga taggagttat gagttcagct
    13081 tggatttcta cattgtcacc aggcataacc attttcactc cttcgggaag atataagctt
    13141 ccggtaacat ccgtagtgcg aaaataaaat tgaggacgat acccactttg gaagggttta
    13201 tggcgtccac cttcttcctc ggttaataca taggtctgac ctatgaattt agtatgagga
    13261 gtaatggatt tgggttttgc cagcaccatt ccacgcacta catctttttt gccgaatcca
    13321 cgtaaaagaa caccaatatt atctccggct tgtgcctcgt caagcagttt acggaacatt
    13381 tccacgcccg tgcaggttgt ttccactgtt tctctaattc caacgcgttc aactttatca
    13441 ccaactttga taacacctct ttctacacgt cctgtggcaa cggttccccg acctggaata
    13501 ctgaaaacat cttccacagg catcaggaag ggcttatcaa ttggacgttc gggaagcgga
    13561 atatagctat ctaccgcatc cagaagagct tgaatttgtt tttcagcttc gggatctcca
    13621 ttgagtgctt taagagcaga accgcgaatt acgggaactt catcacccgg aaattcatac
    13681 ttatcaagca attcacgaac ttccatttcc accagatcaa gcaattcagg gtcgtcaacc
    13741 aggtcgcatt tgttcataaa tacaactatt gctggaacac caacctgacg agcaagcaaa
    13801 atatgctcgc gggtttgtgg cataggtcca tcatcagcac tcacaaccaa aatggcacca
    13861 tccatttgag ctgcaccagt tatcatattt ttaatgtagt cagcatgacc aggacagtca
    13921 atatgagcgt aatgacgttt ttctgtttca tattctacat gggctgtagc aatagttatt
    13981 ccgcgtgctt tttcttccgg agcattatca atactatcaa aagtacggaa tttggctccg
    14041 ccttttttac tcagatacaa agtaatcgct gcagttagcg ttgtttttcc atggtcaata
    14101 tgaccaatcg ttcccacatt cagatgtggt ttagttctaa catatttctc ttttgccatt
    14161 gttcctcctg gacttgaatc acacaagccc gtctcgtttt cttaatttat taaaaaatat
    14221 acaccttcag gtatatattc ctcttaatat catagatgtt gaaattaaaa acatctcttt
    14281 aacttcaaaa gtggagctca agaccggact tgaaccggtg acctcctcct taccaaggag
    14341 gtgctctacc tactgagcta cttgagctta cacttaaaaa ttttggagcg ggaaacgggg
    14401 ttcgaacccg cgaccctcag cttggaaggc tgatgctcta gccaactgag ctactcccgc
    14461 ttttcttttt atatctaaaa tctactggtg gagaggggag gattcgaacc tccgaagtca
    14521 tctgacagca gatttacagt ctgatccctt tgaccactcg ggaacctctc cacgactata
    14581 atttctggag ccgatgaagg gaattgaacc cccaacctat tgattacaaa tcaattgctc
    14641 tacctttgag ctacatcggc a
//
LOCUS       DAOOZV010000046        14615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4152_length_14615_cov_57.347459, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000046 DAOOZV010000000
VERSION     DAOOZV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_14615_cov_57.347459"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="trpS"
                     /locus_tag="PLW08_05685"
     CDS             complement(<1..978)
                     /gene="trpS"
                     /locus_tag="PLW08_05685"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPL52127.1"
                     /translation="MKKIALTGIKPTGIPHIGNYLGAIKPALKLSETCEARYFIADYH
                     ALNACKDPVELKKMTVEIASAWLACGLDPKNTLFYRQSDVPETFELLTILMAFTPKGL
                     MNRAHAYKAMVQNNLEANKDPDDGVNMGLFTYPVLMAADILLFDTDFVPVGTDQFQHI
                     EMAQDIAQSFNYVYKTQALKIPQPIAQEDTKTVVGLDGRKMSKSYNNTIPMFCTEKEL
                     KKLIMSIVTNSQTIEEPKDPDTSNIFALYKNFALPEQVENMRARYLKGGLGWGQAKQE
                     LFEVINAELKPIRERYYYWMNHQEDIWNALKEGSQKARLLASQKIAQLRK"
     gene            complement(975..2306)
                     /locus_tag="PLW08_05690"
     CDS             complement(975..2306)
                     /locus_tag="PLW08_05690"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPL52128.1"
                     /translation="MIKYTIVSLGCPKNLVDSEHFVAIMESYGMQRTDEWEEANILLI
                     NSCSFIFNALEELNCLLADVIDATKKDITKIVVTGCVMNRGYEQLKDYYPEVDAWIPL
                     KDFAAFENYLRENILPSQETPKRLTYTQRVHLEDGNYVYLRIADGCNNKCSYCMIPYI
                     RGKQVSEPIETLIEEAKSMQEYGRELILIAQDTCSYGTDIYGKKALPKLIEALHNQTD
                     YDWIRILYLHPDNFELQWTELWQKFPKLLPYFDIPIQQVSPKIIKAMNRRKSYQELKE
                     LFFHIQKEIPNAVFRTTLMVDYPNETKEDLELLEKFLSEVPFLQGGVFAYSPEAKDLK
                     DNIYDEFDWKKSKKLLFAWEDKFYQIRNQLLEKYVGTIQSALIEDYDPYSEQYVGRLW
                     FQAPEIDGCVYIDKLPADKNSLVEVEIVDVIGDEVMSIFHSNINSNKGITK"
     gene            complement(2303..3553)
                     /locus_tag="PLW08_05695"
     CDS             complement(2303..3553)
                     /locus_tag="PLW08_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPL52129.1"
                     /translation="MSTFKPFKALRPIPDKAKAIASLPYDVMDSDEARIEVQKNPLSF
                     LHVEKPEVDLPLGTDLYDPAVYAKARENLYKYVSDGYMKQDTKPAYYIYKQVMDGRAQ
                     IGLVGLTSVDEYMDGTIKKHELTREEKEADRIRHIDACDAHPSPVFFTYRHQNIIDET
                     VAKVMANKQPEYDFISDDGIQHTLWVMDDPEDIKTIQNAFAALPNLYVADGHHRTASA
                     AKVGLKRREQFPDYTGDEEFNFFMAVIFPDNQLKIYDYNRVVKDLNGLTKDEFLAKVA
                     EKWNVTPIPEGENFAPQKKHNISMYLGGKWYSLEPKPGTWNEKNIVEDLDVSILQNNL
                     LQPILGINDPRTDQRIDFIGGIRGLGELVKRVDSGREKVAFAMYPTSMDELIGIADAG
                     EIMPPKSTWFEPKLRSGLFIHLLK"
     assembly_gap    3568..3667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3668..4918)
                     /locus_tag="PLW08_05700"
     CDS             complement(<3668..4918)
                     /locus_tag="PLW08_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPL52130.1"
                     /translation="MDNLIKDLLAFLSGSKSSFFASLEIRKRLEKAGFTYLPEDKPFK
                     IMRGEKYYLCHQGTTIVAFITGSEKIAKTGFNLAGSHIDYPCLKLKPQSLKTEKGITK
                     IGVQLYGLPIISTWLDRELGIAGKIIVKTAKGYKVEYVDLKKPIAVIPNVAIHLNREV
                     NSGFAYNPQTHLNAILSVSSTGTNPLYSLIAEELKVKEEQIAESDLYLYDFTTPNLLG
                     LKQEMIVSQGLDNLAMTHSILSALLKCEKPQKTAVAIFFNNEEIGSQTSQGANSLLLE
                     EILERICLSQSGSREDFYLALRNSFFISADVANAWHPSFAEKYEPDYAPLMNKGPVIK
                     FDAYNRYASDAESSYRFIQLCEKAKVPYQKFLVRSDATSGVTIGPILSSKLGLKTVDI
                     GNPIWAMHSIRETGGTEDHKYLVQV"
     gene            5100..5552
                     /locus_tag="PLW08_05705"
     CDS             5100..5552
                     /locus_tag="PLW08_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52131.1"
                     /translation="MKVNFKYGIKTYSGTMDEVTYGSYRKNTICIGRKYVKPRLTDQN
                     ALVGGKMKNLAIIYNDVSESYKQELKQYALLNEVNVPKGKLLPNAYALWVKMMFKFAD
                     ADAEHIDLTTLTYSDLQTVGDEILSIASAVENGYMANVPGADKLTANM"
     gene            5565..5729
                     /locus_tag="PLW08_05710"
     CDS             5565..5729
                     /locus_tag="PLW08_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52132.1"
                     /translation="MNGDNILIKELATRINKLVNIPLINEQNEQAFFELIISILLGIF
                     LDEVDKQLLK"
     gene            6133..6285
                     /locus_tag="PLW08_05715"
     CDS             6133..6285
                     /locus_tag="PLW08_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52133.1"
                     /translation="MEDDEDGMLTGKQYQLLIANLFADDKSSYRDKFYGDSCLRRDNK
                     GIFRKK"
     gene            6304..6783
                     /gene="ribE"
                     /locus_tag="PLW08_05720"
     CDS             6304..6783
                     /gene="ribE"
                     /locus_tag="PLW08_05720"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPL52134.1"
                     /translation="MKVMEGKLISKGYKFALVVSRFNEFISSRLLEGALDCLRRHEVN
                     DEDISVIWVPGAFEIPLVAKEAALKKDIDAVICLGAIIRGATPHFEYISAEVTKGIAA
                     VGLETRKPIIYGILTTDSIDQAIERSGTKAGNKGFTAASSALEMLNLLSEMKNGAKA"
     gene            6794..7228
                     /gene="nusB"
                     /locus_tag="PLW08_05725"
     CDS             6794..7228
                     /gene="nusB"
                     /locus_tag="PLW08_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPL52135.1"
                     /translation="MAVQCLYSLEFSEVEKDYREYGLLNEYPDILLSLAEAEHIQPNS
                     PVYAFADELVKNTIINIEQVESEIDKISDNWELEDIALLDRSILCLAAYELLFTDTPA
                     PVVINEAIEIAKKFSSEASGKFINGILDALNKEILQKGKLTN"
     gene            7232..8140
                     /locus_tag="PLW08_05730"
     CDS             7232..8140
                     /locus_tag="PLW08_05730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL52136.1"
                     /translation="MEKIKCSVGIFAHNEAGNIIPLLEAIENQELRETEISEIIVVSS
                     ASTDGTDQLVSSFAETHKNVKLLTQPKREGKSSAINLFLANAQEDIAVIISADVIPAK
                     QTIEKLVSAFKNPTIGATGGRPVPVNEESNYLGYIVHLLWRLHHRMALIYPKLGEMIA
                     FRKVMDSIPKDSAVDEASIEAIIREKGLKLKYIPSAIIKNKGAENLADHIKQRRRIQN
                     GHLWLKAHQHYKVVSQDSGILLKVVLQELKEKPQTFFKLVGAILLEVYCRLLGSWDYY
                     VKGKNPFAWEIARSTKDLNQPKRERK"
     gene            8143..9225
                     /locus_tag="PLW08_05735"
     CDS             8143..9225
                     /locus_tag="PLW08_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL52137.1"
                     /translation="MIYLIFRIIGFWLMKNLGFPHLMPMNYTVSLLYTCNSRCNTCNI
                     WKKQAKNLTLDEYQKIFKNIGKSPYWITFSGGEPFLRNDIVDVVTAIYKISHPRIINI
                     PTNGILVQTIIEKTDAIASACPKAQIIVNISIDGIEEQHDKIRNVPGNYKKAIATFNG
                     LKALKHKNLSVGIHTVISKFNVDSFPAIANELMRLKPDSYITEIAEERAELDTMGTDI
                     APSLVAYKSAIDYLIHRIKNSNFKGMSKVTMAFRIEYYNLVKRIMRDKKQIIPCYSEV
                     ASCQISPDGDIWSCCIKAKSLGNLRKNNYNFRKIWFSPLAEMERRSIHKKKCWCPLAN
                     AAYTNMLLHLPTLFRVSWRSFIHWWS"
     gene            9228..10637
                     /locus_tag="PLW08_05740"
     CDS             9228..10637
                     /locus_tag="PLW08_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPL52138.1"
                     /translation="MVSLQSLHSSPPRSVCEYKASREGRFAWFLLTWHPEKNFVLEEI
                     VAEIKLEILNSKVGSLSRLEIEKWMKQFFAELHWKLYGLLHKTELAEKGISLFFGVLY
                     DNELYFVQFGRIFCLLTDNKKMRHIGRDWHNYQVQSLDGLQLLGLMDSDPVIKPQRVY
                     IGSNQRLIVISGELAQKIFPSVDDPSAIETLIETYATEDNPLWLILEGKEELVKTKRR
                     KLSRLQVTSIVLIVLALLAALYMMVGNRILDQLVYKMRLSLKEEKTLHLEQIPTNLNI
                     NSENLKKYIDKLINLPARNISFSIAWSTELPYNVSLSPSFTLGTIYLVSQNRLSAYDK
                     KSRQLLWTKMLEDEIRSTTVHQGMLILYLANQQIIGLNDEGNILWQQHLPTESITGDR
                     FIPCIIKNSDDPRLDRSIMVIPSKRGISILDPVRGETISSLTFKHDLDALSVYDSFAN
                     CFYAVVGNAVLCVELKIVN"
     gene            10641..11312
                     /locus_tag="PLW08_05745"
     CDS             10641..11312
                     /locus_tag="PLW08_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPL52139.1"
                     /translation="MKRSLIFFCLLLCLPLLAVVIDNPVKIGFARLQYDGGGDWYNDP
                     EVLPNLAKYANLVMGASFPIDQSVVRASDPKLFDYPFLYLTGHGNIRFTDKEVENLRT
                     WMLRGGFLYADDDYGMDESFRREIKRIFPERELVELDPSFPLFYAFFDFSSGLPKIHL
                     HDGKPPQAFGIFDDNGRLMCLYTYETNISDGWADPETHNDPPEIREKALKFGVNILYY
                     VMHKQ"
     gene            11309..12058
                     /locus_tag="PLW08_05750"
     CDS             11309..12058
                     /locus_tag="PLW08_05750"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPL52140.1"
                     /translation="MKIIALSDCHYKFHCASNADKESANKLISFLKSIPGKYDLMVLS
                     GDIFDFWLEGKFTVVKQYFPVLCALSAIKESGTRIVYISGNHDFWFGNFLSDYIGCEI
                     HSDGFTLNTDGKKIRFEHGDTRTFNDLRYQLYRKVIRWKAVHKIASFLHPDLALTIGS
                     RFSRTSRQRKDSQELVDKKSKGLKNYAKWLIEHKKADIVVLGHSHQPVWETMGNGSYL
                     NCGDWITYYSYVEIDAGKPALKEYNIKNEQI"
     gene            12075..12935
                     /locus_tag="PLW08_05755"
     CDS             12075..12935
                     /locus_tag="PLW08_05755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL52141.1"
                     /translation="MRHLNKNTIPILVVLMMITISVGCQTKTPSGEKPAEQLMKEKEI
                     ITNKTKVEMETNYGTIELELWDDLTPKTVQNFVKLARSGYYDGLYFHRVIPDFMIQGG
                     CPNTRDKDRSNDGRGGPGYTFEDECYRNGEEIIGKIDTNQKAEAVWNKIIVPHIQNNR
                     TPNAEIAAIAKECQSAHSLEPVMKYDLKFYQEKTGIMDKVYVQELIAPVDYGTICMAN
                     SGPNTNGSQFFIVTKKDGAHWLDGQHTVFGRVTKGMEVAHKIENLPRDKNDNPNPENQ
                     AIITRISFPK"
     gene            12948..13391
                     /locus_tag="PLW08_05760"
     CDS             12948..13391
                     /locus_tag="PLW08_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPL52142.1"
                     /translation="MSRANESALQQEIMAWIKQNQYVYLATVDKNRPRVRPVVLFIYD
                     DRFFVTTFSGDSKVSQIQNNKNIEVCLPLYEDGQTGYIRLSGTASIVHNATLKAEASE
                     RCFFFDDYFSGYDDPDYTLIELDFDYAEYLRPGESYSQGCNLKRY"
BASE COUNT         4449 a         2803 c         2882 g         4381 t
ORIGIN      
        1 ttttctcaac tgtgctattt tttgggaagc aagaaggcgt gctttttggc tgccctcttt
       61 tagggcattc cagatatcct cttgatgatt catccagtaa taatagcgtt cgcgaatagg
      121 tttaagttca gcatttatga cttcaaaaag ttcctgtttt gcctgtcccc aacccaagcc
      181 acctttgaga taacgagctc tcatattttc tacctgttcg ggtagggcaa aatttttata
      241 gagggcaaaa atatttgagg tatcggggtc tttaggttct tcaattgttt ggctattggt
      301 tacaatgctc ataatcaatt ttttcagttc tttttcggtg cagaacatcg gaatggtgtt
      361 attgtaactt ttgctcattt tgcgtccatc cagtcctaca acggtttttg tatcttcctg
      421 agctatcggt tgcggaattt ttagtgcttg cgttttatac acatagttga agctttgagc
      481 aatatcctgt gccatttcta tatgctgaaa ttgatctgtt cccacaggaa caaaatccgt
      541 atcaaacagc aaaatatccg cagccattaa aaccggatag gtaaaaagac ccatattcac
      601 tccgtcatca ggatccttat ttgcttccag gttgttttgc accattgctt tatatgcatg
      661 ggcacgattc attaaaccct tgggagtaaa tgccataaga atcgttagca gttcaaatgt
      721 ttccggcaca tcactttggc gataaaatag tgtatttttg gggtctaaac cgcaagctaa
      781 ccaggcagaa gcaatttcca cagtcatttt tttcagttct acagggtctt tacaggcatt
      841 taaagcatgg taatcggcaa taaaataacg cgcttcacag gtctcagaaa gttttaaagc
      901 gggttttatg gctcccagat aatttccaat gtggggaatt ccggttggtt taattccggt
      961 taaagctatt tttttcattt agttatccct ttattgctat ttatattaga gtgaaaaatg
     1021 ctcataactt cgtctccgat aacatctaca atttccactt ccacgagaga atttttatcg
     1081 gcaggaagtt tatctatata cacacagccg tcaatttcag gggcttgaaa ccataggcgt
     1141 cctacatatt gttcgctata gggatcataa tcttctatta aagctgattg aatagttcca
     1201 acatattttt ccagaagttg attccttatt tgataaaatt tatcttccca agcgaacaac
     1261 aattttttac tttttttcca gtcaaattca tcgtagatat tatctttcag gtcttttgct
     1321 tcgggagaat aagcaaatac acctccctgc aagaaaggta cttcggaaag aaatttttcc
     1381 agaagttcta aatcctcttt tgtttcattc ggataatcaa ccatcaaagt agtgcggaaa
     1441 actgcattag ggatttcctt ttgaatatgg aaaaagagtt ctttcagttc ctgatagctt
     1501 tttcttctat tcattgcctt aatgattttg gggctcactt gttgaatagg gatatcaaaa
     1561 tagggcaata atttaggaaa cttttgccag agctctgtcc attgcaattc aaagttatcc
     1621 ggatgcaaat agagaatgcg aatccaatcg taatctgttt ggttatgcaa tgcctcaatc
     1681 aatttgggaa gggctttttt gccataaata tcggttccgt aagaacaggt atcctgagct
     1741 attaaaataa gctctctgcc atattcctgc attgattttg cctcttcaat tagggtctca
     1801 atcggttcgg aaacctgttt tcctctgata taaggaatca tacaatagga gcatttatta
     1861 ttgcaaccgt ctgctatccg caaatataca taattgccat cttccaaatg aactctttgc
     1921 gtgtaggtta acctttttgg ggtttcctga gaaggcagaa tattttcccg cagatagttt
     1981 tcaaaagctg caaagtcctt taacggaatc caggcatcca cttccggata ataatcttta
     2041 agttgttcgt aaccacgatt cattacacag ccggtaacaa ctattttagt aatatccttt
     2101 ttcgtagcat ctataacatc cgccaataaa caattcagtt cttccagagc attgaagata
     2161 aaggaacagc tgttaatcaa taatatattt gcttcttccc attcatccgt tctttgcatt
     2221 ccgtagcttt ccataatggc tacaaagtgt tcgctatcca ctaaattttt gggacagccc
     2281 aaggaaacaa tcgtatattt tatcatttaa gtaagtgaat aaacagtcca ctgcgtaatt
     2341 taggttcaaa ccaagttgat ttgggaggca taatttctcc tgcatcagca attcctataa
     2401 gttcatccat acttgtagga tacatagcaa aagcaacttt ttctcttccg ctatcaactc
     2461 ttttaaccaa ttcacccaga cctctgattc caccgataaa atcaattctt tgatcggtgc
     2521 gcgggtcatt gatacctaaa atgggttgca aaagattatt ctgtaaaatg gaaacatcaa
     2581 ggtcttccac aatgtttttc tcgttccagg ttcccggttt gggttctaaa ctataccatt
     2641 ttcccccaag atacatactg atattgtgtt tcttttgagg agcaaagttt tctccttcag
     2701 ggatgggggt aacattccat ttttcagcta ctttggctaa aaattcgtct ttggttaagc
     2761 cattcaggtc tttaaccacc cgattataat cataaatctt caattgatta tccggaaaga
     2821 taaccgccat aaaaaagttg aattcttcgt cgcctgtata atcgggaaac tgctcgcgcc
     2881 ttttcaatcc aacttttgcc gcgctggcag ttctgtgatg tccatctgcc acatacaaat
     2941 tcggtaaggc ggcaaaggca ttttgaatgg tctttatatc ctcaggatca tccatcaccc
     3001 aaagtgtatg ctgaataccg tcatcgctta taaaatcata ttctggctgt ttattagcca
     3061 taaccttagc tacagtttca tcaataatat tttgatgacg ataggtaaaa aacacaggag
     3121 aaggatgtgc atcacaagca tcaatatgcc gaatgcggtc tgcttctttt tcttctcttg
     3181 ttaattcatg ctttttgata gtgccatcca tatattcatc tacggaagta aggcctacca
     3241 aaccaatttg agcacggcca tccataactt gtttataaat gtaataagct ggttttgtat
     3301 cctgtttcat atagccgtcg gaaacatatt tatagaggtt ttctctggct ttggcataaa
     3361 cagctggatc gtataaatcg gttcccaagg gaagatcaac ttccggtttt tctacatgga
     3421 gaaagcttaa aggatttttt tgcacttcta tgcgtgcttc atctgaatcc ataacatcat
     3481 aaggcaaaga agcaattgcc tttgctttgt ccggaatagg acgcagggct ttgaagggtt
     3541 tgaaagttga catttatata ctcctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnaac ttggacaaga tatttatggt cttcagttcc tcctgtttcc cggatggaat
     3721 gcattgccca aattggattt ccgatatcaa ctgtcttcag ccccaatttt gaagataaaa
     3781 taggaccaat tgtaactcca ctggtggcat cgctacgcac taaaaacttt tgataaggaa
     3841 cttttgcctt ttcacaaagc tgaataaagc gataggagct ttctgcgtcc gaggcgtatc
     3901 tgttataagc atcaaactta ataactggtc ccttattcat cagaggagca taatccggct
     3961 catatttttc ggcgaaagaa ggatgccagg catttgctac atcagcacta ataaaaaagc
     4021 tgttgcgtaa agccaggtaa aaatcttctc gggaaccgct ttgactgagg caaattcgtt
     4081 ctaaaatttc ctccagaagt aaggaatttg ctccctgaga ggtctgcgaa ccgatttctt
     4141 cattattaaa aaagatagct actgctgttt tttgaggttt ctcacattta agaagggcgg
     4201 aaagaataga atgagtcata gccaggttat caagaccttg agaaacaatc atttcctgtt
     4261 tcaatcccaa taaattcggg gttgtaaaat catacaaata taagtcactt tcggcaatct
     4321 gttcttcctt aaccttaagt tcctccgcga taagagaata aagtggattt gtgcctgtag
     4381 aactaacact gagtatagca tttaaatggg tctgtggatt ataagcaaac ccactattta
     4441 cttcccgatt caaatgtata gccacattgg gaataacggc tatgggcttt tttaagtcca
     4501 catattccac tttataaccc tttgccgttt ttactataat ttttccggca attcccaatt
     4561 cgcggtctaa ccaagtgctg ataataggta agccataaag ctgaacccca attttagtga
     4621 tccctttttc ggttttcaaa ctttggggtt tgagctttag acagggataa tcaatgtgac
     4681 ttccggcaag gttgaaacct gttttggcga ttttttcact acctgttata aaagcaacaa
     4741 tagtagttcc ctgatgacag agataatatt tttcgcctct cataatcttg aaaggtttat
     4801 cttcaggaag ataggtaaaa cctgcttttt ccaaacgctt ccgaatttcc aaactggcaa
     4861 aaaaactgct tttactacca gaaaggaaag cgagtaaatc cttaattaaa ttatccatta
     4921 tctaaactcc aatatttatt tattttaaat gccagatttt taatctccgc tttcgtgtca
     4981 agaagggcaa tttgagaaaa attccaaatt tgtcaccatc tttcattttt gtcaccacct
     5041 atataagtga aggcaagatt tccggaaacg cctaaaacaa aaaacaaagg agaaactaaa
     5101 tgaaagtaaa cttcaagtac ggaatcaaaa cttactccgg cactatggat gaagtaacct
     5161 acggcagcta tcgtaaaaat actatctgca taggccgtaa gtatgtaaaa ccccgtctta
     5221 ccgaccaaaa tgctttagtt ggcggcaaga tgaaaaacct ggcaattatc tataatgatg
     5281 tttcagagag ttataaacaa gagctgaaac aatatgccct tctaaacgag gtaaatgttc
     5341 ctaaaggcaa attgcttcca aatgcatacg ctctttgggt taaaatgatg tttaagtttg
     5401 ccgatgctga tgcggaacat atagacctga ctaccctaac ctattccgat ctgcagaccg
     5461 ttggagatga aatcctttct atcgcttctg ctgtggaaaa tggttatatg gctaatgttc
     5521 ccggtgcgga taaactgacc gctaatatgt aaaggaggta aataatgaac ggtgataata
     5581 tcctcataaa agaactcgct acccggatca ataaattggt aaacatccct ttgatcaatg
     5641 agcagaatga acaggcattc tttgaactga taatctctat cctgctcggt atcttcttgg
     5701 atgaagtgga taaacagtta ctcaagtaac ataaactcct aaccacctgt aaaagtgccc
     5761 cggtttttgc cggggctttt tctgtgccct ttttatttcc ggctgctggc gtaaaccttc
     5821 cttggtaaag gaagcaggta aaaaagggta agagggagaa ggaagaatta tcaataataa
     5881 tctgtacccc taacgggaac ttaatttttc tctgtgctat ccgggaaata gaggagagat
     5941 aaaattgaag ctcggcaaga agcataagca ccattaaact tagagccgga attataaggg
     6001 gataggagct aagaacttct tgcttacaag gagctaatgc ttactaattt gccaggagaa
     6061 ttcgttcctc cggaagattt tccttgaaca aattatagga gctgaaataa taggttttag
     6121 ggaaaaatta ggatggaaga tgatgaggat ggaatgctga caggcaaaca atatcagctc
     6181 ttaattgcta atttatttgc agacgataaa agcagttata gggataaatt ctatggtgat
     6241 tcctgcttgc gcagagataa caaagggatt tttaggaaaa aataatctac aaaggagcca
     6301 aagatgaaag ttatggaagg aaagcttatt tccaagggct ataaatttgc tcttgtagtc
     6361 agccgtttta acgaatttat cagttcccga ttgctggaag gagctttaga ttgcctcagg
     6421 cgtcacgaag taaacgatga agatatcagc gtaatatggg ttcccggtgc ttttgaaatt
     6481 ccactggtgg caaaagaagc cgcattaaaa aaagatattg atgccgtaat ctgtttggga
     6541 gcaataattc ggggtgcaac tccccacttt gaatatatca gtgcagaagt cactaaaggc
     6601 attgctgctg tcggtttgga aacccgcaag ccgattattt acggtatttt aacaaccgat
     6661 agtattgacc aggcaataga acgctctgga accaaagccg gtaacaaggg ttttacggca
     6721 gccagttctg ctttggaaat gcttaatctt ttatctgaaa tgaaaaatgg ggcaaaggcg
     6781 taaagcacga gaaatggctg ttcagtgcct ttattctctg gaattttcag aagtggaaaa
     6841 ggactatcgg gaatatggtc tcttgaatga atatcccgat attctgcttt ctttggcaga
     6901 agcggaacat attcaaccca attccccggt ctatgctttt gcggatgaac tggtaaaaaa
     6961 cacgattatc aatattgaac aggtggaatc ggaaattgat aaaataagcg acaattggga
     7021 gctggaagat attgccctgc ttgatcgcag cattttatgc ctggctgcct acgaactttt
     7081 gtttacagat actccggcac cagtagtaat taatgaggca attgagattg ccaagaaatt
     7141 cagcagtgaa gcatctggta aattcatcaa cggcattctg gatgctttaa acaaggaaat
     7201 acttcaaaag ggaaaactaa caaattaggt aatggaaaaa ataaaatgtt ctgtcggaat
     7261 atttgcccat aacgaagccg gcaatatcat tcctttactg gaagcaattg aaaatcagga
     7321 attgcgggag accgaaatca gcgaaataat tgttgtttcc agtgccagca ccgatggaac
     7381 agaccaactg gtaagctctt ttgccgaaac tcataaaaat gtaaagcttc tcactcagcc
     7441 aaaaagggaa ggcaagtcct ctgccattaa tcttttttta gccaatgccc aagaggatat
     7501 tgccgtaatt ataagtgctg atgttattcc tgccaaacag accatagaga aattggtttc
     7561 tgcttttaaa aatcctacaa ttggggcgac gggaggtcgt cctgttccgg taaatgagga
     7621 aagcaattat ctcggttata tagttcatct tttgtggcgt ttacatcatc ggatggcatt
     7681 gatttatccc aaattgggag aaatgattgc cttccgtaaa gtgatggata gtattcctaa
     7741 agatagtgca gttgacgaag ccagcattga agcaataata agagagaagg gcttgaaact
     7801 taaatacata ccttctgcca ttattaaaaa caaaggagct gaaaatctgg cagaccatat
     7861 aaaacagcgg cgtcgtattc agaacggtca tttatggctg aaagcgcatc agcattataa
     7921 agttgtttct caggatagcg gtattttatt gaaagttgtt ttacaggagt tgaaagaaaa
     7981 gccccagacc ttttttaaat tggtgggagc tattcttctg gaagtttact gccgtctgct
     8041 tggcagttgg gattattatg tgaaaggaaa aaatcccttt gcctgggaaa tagcgcgctc
     8101 cacaaaggac ttaaatcaac cgaaaaggga aagaaaatag aaatgattta cctcatattt
     8161 cgcatcattg gtttctggct gatgaaaaac ctgggttttc ctcatctgat gccaatgaat
     8221 tatacagtca gtttgcttta cacttgcaat tcgcgttgta atacctgtaa tatctggaaa
     8281 aagcaggcaa aaaatttaac cttggatgaa tatcagaaaa ttttcaaaaa tataggcaaa
     8341 tcaccatact ggattacttt cagcggagga gaaccatttt tacgcaatga tattgtagat
     8401 gtggtaaccg ctatctacaa aatcagtcat ccccgcatta ttaatattcc cacaaacggt
     8461 attttggttc aaaccattat agaaaaaacg gatgctattg ccagtgcctg tcctaaagct
     8521 caaataatag ttaatatttc cattgatggc atagaagaac agcacgataa aatccgcaat
     8581 gttcccggaa actacaaaaa agcaattgct acttttaacg gtttgaaagc cctgaaacac
     8641 aaaaacctca gcgtaggcat tcatactgtt atttccaagt tcaatgttga tagttttccc
     8701 gctattgcta atgaattgat gcgtcttaag cccgatagct acattactga aatagcggaa
     8761 gaaagagcgg aactggatac gatgggaaca gatatagctc cttccttagt tgcctataaa
     8821 agcgctattg attatcttat tcatagaatc aaaaacagta actttaaagg aatgagcaag
     8881 gttactatgg cttttcgcat tgaatattac aatctggtga aaaggattat gcgtgataaa
     8941 aagcagatta ttccctgtta ttcagaagtt gcctcttgtc agatttctcc ggatggcgat
     9001 atttggagct gttgcattaa agccaagtcc ttgggaaacc tgcgaaaaaa taactataac
     9061 tttcgtaaga tttggttttc tccgcttgcc gaaatggaac gtcgttccat tcataaaaag
     9121 aagtgctggt gtcctttagc caatgctgct tacactaata tgctgttaca tctgccaact
     9181 ctttttcgtg tttcctggcg cagttttatt cattggtgga gctaaaaatg gtttctttac
     9241 agagtttgca ctcctctcct ccccgcagcg tatgtgaata taaggcatcc cgtgaaggac
     9301 gctttgcctg gtttttgctt acctggcatc cagagaagaa ttttgtactg gaagaaattg
     9361 ttgccgaaat caagctggaa attcttaatt ccaaagttgg ctcgctttcg cgtctggaaa
     9421 ttgagaaatg gatgaaacag ttctttgccg aactgcattg gaaactctat ggactattgc
     9481 ataagaccga actggcagaa aaaggtattt ctctcttttt tggtgtcctc tatgataacg
     9541 agctttattt tgttcagttt ggcagaatat tttgtctttt aaccgataac aaaaaaatga
     9601 gacatatcgg aagggactgg cataattatc aggttcagtc attggacggt ttacagctgc
     9661 ttggtttaat ggacagtgat cctgttatta agccacaacg ggtgtatatt ggcagtaatc
     9721 aacgattgat cgttatttca ggggaactgg cacaaaagat ttttcccagt gttgatgatc
     9781 cttctgccat agagacatta attgagacct acgctacgga ggataatcct ctttggctga
     9841 ttttggaagg taaagaggaa ttggttaaaa caaagcgcag aaagctatct cgtttgcaag
     9901 taaccagtat cgttcttatt gttttagctt tgttggctgc tctgtatatg atggttggaa
     9961 atcgtattct tgatcaactg gtgtataaaa tgcgtctatc actcaaagag gaaaaaacct
    10021 tacatttgga acaaattcct accaatctga atatcaatag cgagaacctg aaaaaatata
    10081 tagataaact gataaatctg cccgcccgca atatttcttt ctccattgcc tggagcacag
    10141 aactaccata taatgttagt ttgtcacctt cttttacatt aggcacaatt tatctggtat
    10201 ctcaaaaccg acttagcgct tacgataaga agtcccgtca gttgctctgg actaaaatgc
    10261 tggaggatga aattcgctct acaacagtcc atcagggaat gctaatcttg tatctggcaa
    10321 atcagcaaat tatcggtctg aatgatgagg gaaatattct ctggcaacag catctgccta
    10381 cagaaagcat aaccggtgat cggttcattc cctgcatcat taaaaacagc gatgatccgc
    10441 gtttggatag aagtataatg gttattcctt ccaagagggg aatcagcatt cttgatccgg
    10501 ttcggggtga aaccattagc agtttaactt ttaagcacga tttggatgct ctttctgttt
    10561 atgacagctt tgccaattgt ttttacgcgg ttgtaggcaa tgcggtttta tgtgtagaat
    10621 taaagattgt gaattaagct atgaaacgct ctcttatttt tttctgcctg ttactttgtc
    10681 tgcctttgct ggcagttgta attgataatc ctgttaaaat tggctttgct cgtttgcaat
    10741 atgacggggg aggcgattgg tataacgatc ccgaagtgct tcccaatctg gcaaaatatg
    10801 ccaatttggt tatgggtgca agttttccta ttgaccaaag cgtagttaga gcttccgatc
    10861 ccaagctttt tgattatcct tttctttatt taaccggtca cgggaatatc aggtttacgg
    10921 ataaagaagt ggaaaacctc agaacctgga tgctccgagg cggttttttg tatgctgatg
    10981 atgattatgg tatggatgag tccttccgca gagaaataaa acgcattttc ccggaaagag
    11041 aacttgttga attagaccct tcttttcccc tattttatgc cttttttgat ttcagtagcg
    11101 gacttcccaa aattcatttg cacgatggta aacctcccca ggcatttggt atttttgatg
    11161 ataacggacg cttgatgtgt ttatatactt atgaaaccaa tatcagtgac ggttgggctg
    11221 atcctgaaac gcataatgac ccacccgaga tcagggaaaa agctcttaag ttcggtgtaa
    11281 atattcttta ttatgtaatg cacaaacaat gaaaataatt gctctttccg attgccatta
    11341 taagtttcac tgcgcaagca atgcagataa agaaagcgcc aacaaactaa tcagttttct
    11401 taaaagcatt ccgggaaaat atgatttaat ggtgctttcc ggggatattt ttgatttctg
    11461 gctggaagga aaatttaccg tagtgaaaca atattttcct gtcctttgtg ccctatccgc
    11521 tatcaaagaa agtggaactc gcatagttta tatcagtggt aatcacgatt tctggttcgg
    11581 aaacttttta tccgattata ttggttgtga aattcattct gatggcttta ctcttaatac
    11641 cgacggcaaa aaaatccgtt ttgaacatgg cgatacccgc acttttaacg atttgcgtta
    11701 tcagctttac cgtaaagtaa ttcgctggaa agctgtccat aaaattgctt ctttcctgca
    11761 tcccgatttg gctttaacta ttggtagtcg cttttcccgc accagcaggc aaaggaaaga
    11821 tagccaggaa cttgttgaca aaaaaagcaa gggcttaaaa aattacgcca aatggttgat
    11881 tgaacataaa aaagccgata ttgtggtttt gggacatagt caccagcccg tttgggaaac
    11941 tatgggaaac ggctcttacc ttaattgcgg tgactggata acctattatt cttatgtgga
    12001 aattgatgca ggaaaaccag ctctgaaaga gtataatatt aaaaatgaac aaatataaca
    12061 caaaggagta tattatgagg cacttaaaca aaaacactat cccgatcctg gttgttttaa
    12121 tgatgattac aatcagtgtc ggatgccaaa caaaaacacc ttccggagaa aagccggcgg
    12181 agcaattgat gaaagagaaa gaaattatta ctaacaaaac aaaagttgaa atggaaacaa
    12241 attatggcac cattgaactg gaactttggg atgatcttac tcccaaaacc gttcagaatt
    12301 ttgtaaaact tgccagatcc ggatattacg acggcttata cttccatcgc gtcattccgg
    12361 attttatgat tcagggtgga tgccccaata cgagagataa agaccgcagc aatgatggac
    12421 gcggcggtcc aggttatact tttgaagatg aatgttatag gaatggagaa gaaattatcg
    12481 gtaaaataga taccaatcag aaagctgaag ctgtctggaa taaaattatt gtgccacaca
    12541 ttcaaaataa tcgcactccc aatgcggaaa ttgctgctat tgctaaagaa tgtcaatcag
    12601 cacatagttt agagcccgta atgaaatatg atctcaagtt ctatcaggaa aaaaccggga
    12661 ttatggataa ggtctatgtg caggaattaa ttgctccagt ggactatgga acgatctgta
    12721 tggcaaattc aggtcccaat accaatggca gtcagttctt tatcgtcact aaaaaagatg
    12781 gagctcactg gctggatggt cagcataccg ttttcggcag agttactaaa ggaatggagg
    12841 tggctcataa aattgagaac ctgcctcgcg ataaaaacga taaccccaac cccgaaaatc
    12901 aggcaattat tacccgtatt tcttttccca aataataaag aactaaaatg agccgcgcca
    12961 acgaatccgc tttgcaacaa gaaattatgg cttggataaa acaaaatcag tatgtttatc
    13021 tggctactgt agataaaaac cgacccagag ttcgtcccgt tgtcctattt atctacgatg
    13081 accgcttttt tgttactacc ttcagcggag attccaaggt gagccaaatt caaaacaata
    13141 aaaatataga ggtctgcctt ccgctttacg aagatggcca aaccggctat attcgcttaa
    13201 gtggaactgc ctctattgtc cataatgcaa cgctcaaagc tgaagcatct gagcgttgct
    13261 tcttttttga tgactatttt tccggctatg atgaccccga ttatactctt atagaattgg
    13321 attttgacta tgcagaatac ctaagacccg gagaaagcta tagccaaggg tgcaatctga
    13381 aacgctacta aaaatgaaga atggattccc aaggaaacct ggttgaaaat gagaattgat
    13441 aagggttgag aaggttgaaa ggggctgtct cacaacctcc ccaaagtcca ttttttgcca
    13501 ttttcaggta tacaaagatg tatcctaaag aggagattgt tcctaaaaat ggcttatatt
    13561 tactgtctca aaactaactt ttagtttcgg gacaaccccg acacaatgat agcgatgggt
    13621 gcgtaagccc ctcgggaaat gtaaaccccg agttcctttt ttccgaaaga gttccatagg
    13681 aacgacagat aataacgacg ggttttaacc cggcgaaaag agtgtaaata agcaacatat
    13741 ctccttgtat ccgcaagagt tccgtaggaa cgacagatac taacaacggg ttttaacccg
    13801 gtgcaaagag cagctaaaaa caacatatct ccttttttcc gaaagagttc cataggaacg
    13861 acaggtatta acaacgggtt tcaacccggt gtgaatagag gataaaaaca acatatctcc
    13921 ttttatcaga atgagttccg taggaacgac agatactaac aacgggtatt aacctggtgc
    13981 aaagagcaga taaaaacaac atatctcctt ttttccgaaa gaattccaca ggaacgacag
    14041 atacccaaga ttttcatttc aataagaaaa taacatttta taataatagt atttaaatat
    14101 ggagttaccg aatcccgatt agccaaaagg gaagaacatt atcatttacc gccctccaat
    14161 taattgcttc gcctttaatt tattatcttt ttcagcttgc ttcatcttct tataaccaag
    14221 taacaaccaa gtaacaactc cgtaacgaga caacggaagt gttacggaag tgttacggag
    14281 tcgttacaga atttactata tatcaagcaa ataactatca agatttatta gctctgggag
    14341 gaatgggatg aaagggattt tcaggatatt tatactggtg aacagtggaa aaaaatagat
    14401 ggttgtttct ttttttttac gaggggctta caccaccatc gctatgattg tgtcggggct
    14461 gtcccgatac aacgatagag atgggtgtgt aaaccactcg gataaagaag gtaaagattt
    14521 tcccgttatt ccaaatcaaa tcagaaaata tttctaagcg tcccttgctt caaacccaac
    14581 atcaacccaa cctttaggat gggtagatgc agagt
//
LOCUS       DAOOZV010000047        14364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4252_length_14364_cov_62.958278, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000047 DAOOZV010000000
VERSION     DAOOZV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4252_length_14364_cov_62.958278"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2814
                     /locus_tag="PLW08_05765"
     CDS             <1..2814
                     /locus_tag="PLW08_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPL52143.1"
                     /translation="FTEILLDNIKNNQTNFIYDLNEFLERYKKDNKVEEINDFTEDDL
                     NKIAFWMATGSGKTLIMHINYYQFLHYKLFSPDSILLITPNEGLSKQHYEELTKSGIP
                     CRLYSENFRFSDSVKTEGEVLVIEMTKLVETKKGGGVTISINAFEGRKLIFVDEGHKG
                     KKAEEQKWAKLRDSLAENGMVFEYSATFGQILSEKNKVTLQEYAKAIIFDYSYKYFYL
                     DGYGKDFSVINAKNIKVDETRFQETMFLANLLDFYEQSLIFADRKGLAEKQNLEKPLW
                     IFVGTTVTGSEEESDVVQIVNFLHKVIKDPAWVNSKLTAILTGKAEFKNESGEDIFAN
                     KFTYIKEKGLNIEDLFTKIFGGKGGFGIYELKSAKGEFGLKTGENQYFGVINIGDVSS
                     FKKQLEKIGLPVETDAISESLFDHIKAPNSSVNILIGSKKFIEGWDTWRVSSMGLLNI
                     GTGQGPQIIQLFGRGVRLKGQNMTLKRSMEKDIKQLENLNIYGIRADYLSKFLEVLSK
                     EEMDLETIEIPVQPNNVEKWETLYTLAKKDVDFNKEKVLFLKEENFSITLDLMPKLSA
                     YLSQKRNAQGIASSNIAINTNELKSATDDDPRKKLNLFFDWNKIWLELYEYKLERGYW
                     NLILNRDFIKNYLLYSDKYKILATPETLQVNSIEDIPRLENIALQLMKKYIDQFYNFH
                     LKKYEMEHLYYAPVTEDSALSVFAKPDDGYNYTIQISKNKENNELIANIKALVQDLDK
                     LMKEETNELPRIYFDKHLYLPILLRDTKIENMHPEGLVESEKVFISGLKNYLVLKKNE
                     FTNKEIYLLRNFPKSGIGFFNLSGFYPDFIMWVKYQNKQIICFLDPKGLEHQKSLDSE
                     KVQLHKEIKEIEKQLGNKDIILESFVLSITPYDSLITGLVNPASQKEYENNHILFLGS
                     IWSGWQDRLFDILEKSISANKSE"
     gene            complement(2850..3233)
                     /locus_tag="PLW08_05770"
     CDS             complement(2850..3233)
                     /locus_tag="PLW08_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52144.1"
                     /translation="MEDKTKALCEELMLIIMIIFNERGKGILFYSLYPVFNYGFGTIS
                     DLLKVLVNEGYVNVSFDLNEQPEDYPDEFLPKEEQIKKLHLCNHYTTYIFPTSEGLQF
                     VKNNLKQAIEENREIKFTGLLHPLK"
     gene            4356..5636
                     /locus_tag="PLW08_05775"
     CDS             4356..5636
                     /locus_tag="PLW08_05775"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPL52145.1"
                     /translation="MKDTENILALLKQEVSPALGCTEPAAVALAGAYASSVLKDTIIS
                     ADLIVSPNILKNGMGVGIPNTNLMGLEIAFALGILVAEPEKGLEVLLDLKAETINAGK
                     QMLAEKRINTKMKETEEKIWIEASLKTAKSESKAILRWRHNWLYHWEKDKHILQHNEI
                     GAEEAPHLISPSAFTLKDYYDFCRETEIDKLELIDLGLILNRRIADFGLSNKSGINVG
                     KMIMEQINSGLLGDDIHHYAMALTAAATDARMSGCNLPVISNSGSGNQGLAITLPIIA
                     VAEKLGSNQEELIRALAMGHLVSVHIKQKIGILSCICGCLSASAGAASGIVMLLKGTY
                     PQIEYAIKNMIADTAGMVCDGAKEGCSLKVATTTSAAVQAALLAKTGICVSSNDGIIT
                     DSIEGTIDNLAYFVNKGMQDADSTILNIMLNKIK"
     gene            5652..7661
                     /locus_tag="PLW08_05780"
     CDS             5652..7661
                     /locus_tag="PLW08_05780"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPL52146.1"
                     /translation="MPNRKYEVHLPAELPPEPTFEPNIRRAPDRGLDLSKKDIALALK
                     NALRYIPKELHSVLAPEFLQELKTRGRIYGYRYRPQGHIYGKPIDEYKGITPARAIQV
                     MIDNNLDFDVALYPYELVTYGESGQVCQNWMQYRLIKQYLEIMTENQTLVVASGHPLG
                     LFPSRPEAPRVISTNGLMVGKWDNPIEFKRLTALGVANYGQMTAGGWMYIGPQGIVNG
                     TYITLLNAGRKYLGIPEDQNLAGVLYVSSGLGGMSGAQSKAIEIAGGIGIIAEVDKSR
                     IETRYNQGWVSKVSSNLAEIFNWVDEARHTQKPLSIAYYGNIVDLLEYIDQHNIKVEL
                     LSDQTSCHAVYEGGYTPVGITYEEGRKLLAENIELFKQKVDASLRRHFNVLKNLTAKG
                     SKFWDYGNSFMASVFESGITEIARNGINTNEGFIWPSYVEDIMGPICFDYGYGPFRWI
                     CLSRKDEDLHKTDLAAISLIDPNRSAMDRDNYYWISTAEENKLVVGTKARILYADEES
                     RIKLGLKFNEMVRKGEIGPVMLGRDHHDVSGTDSPFRETANIYDGSNLMAEMATHCFA
                     GNIARGMTMVVLSNGGGVGVSRCINGGNGIVLDGSERMDEVVKSGLSWDVMGGIARRA
                     WAQNEGAIKTGTAWNEKHSAEGNITLAEKVDEEMVKYLVNKEFGA"
     gene            7664..9058
                     /locus_tag="PLW08_05785"
     CDS             7664..9058
                     /locus_tag="PLW08_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPL52147.1"
                     /translation="MSTPFIRLMKQEGIRDEVIRTFEAYYQKLVKGEKGLIGEDAIRP
                     PSENNLIDYQQIKHLSRTSILKNTVVIKLNGGLGTSMGLSKAKSLLPVKNNLNFLDII
                     SRQVLTLRSLSGYDVLLLFMNSFNTEADTLKYLEKYPDLGKQDLPLSFLQNKYPRIRQ
                     DNLMPYENKERKLMWNPPGHGDIYTALGDLLDKMIAKDYRYAFVSNADNLGAVVDTSI
                     PAYMENNNIPFVMEVCLRSPMDKKGGHLCEDKSGQLLLREIAQCPEEDLPRFQDIDYY
                     KYFNTNNIWIDLRALEWQMIANEGLMLLPLIVNPKVVDGTPVYQLETAMGSAISVFNN
                     SKALVVSRERFVPVKKTNELLALWSDAYELNEQYQIVLKRGVEKAPVIELDEKYYGKI
                     EDLQKRFSKGIPSLSECKELKIYGDVSFGEDVICEGKVTLKASSPVFVQNCLLSGEVN
                     LDSTINKSKDRTGG"
     gene            9060..10292
                     /locus_tag="PLW08_05790"
     CDS             9060..10292
                     /locus_tag="PLW08_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPL52148.1"
                     /translation="MVSQAAKIRLGIFLVIGAILIILFAIVVAGSRLVEKKDIYYIEF
                     EDYSVSGLQVGSSVNYRGIKIGRVEDVKINPKDVTKIIITISVNRGTPIKEDTEAVLL
                     LSGITGLKNVELRGGTNEAKLLKPKSYIKTGTTVLEDISERAKSIVDKIDMIAANLSA
                     ITGEENRNNISTILAQTSLILKDTHENLATTMQSISRIANNTADLTEELSKDLSAVTE
                     NLTKNLDAITSSATTNIQNVSETSQASLISVTNNVNQQLEELTAKLETSLTQLTNDGS
                     ALIREANLKVTTVGEHSDQMILEATREILTISNNINRALDQVNAILYTPGFDSLMTNL
                     GTLCGELSKTDLKGMVTELSTTIQKTGTLVSNLNRVVTRGQGDLLEILSTLTETSENL
                     NEFSRQIADTPAILLRGN"
     gene            10301..12490
                     /locus_tag="PLW08_05795"
     CDS             10301..12490
                     /locus_tag="PLW08_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPL52149.1"
                     /translation="MLKRKCLFIFSGFLAILLLVTGCLQKVERTPVNYYVLEYQPGTE
                     KPELRRTTNTGKNLEVLDTVLNRTYDRSQIVVKENFYNVKFLQTDVWATRLRDAIPNL
                     IVQRLKAYNIFGTVSRGEQLEKNPHYFLETKVYNIEKIAGTEPRAYLNMEFVLRDSTS
                     ERIVFSHKGERTMELIDPSMIYLVQAFNEMIMEETDLFAAKCNLYFSGLPVESKSLAA
                     STSPIAKYVYEEMIVRETDFDFGELMVITKTQTEEQIRYTIEELDSLNTVISTDELIM
                     GVPALLTPGNYRVIIGEMGDLIIPVKILPRQRTVVKPNWGELKIVVMDESKARVRMGY
                     DLWKKNTDGEGYKIYSGGMFSMGEDEVGAVDKLWILPPGSYLVKLGGGSWSDLRDFAT
                     VTLAEGEKKTLTMIVDPAAEGNVLIGAGVFADEDIGLGSKRIHRGAIHGNISLTSNNN
                     VDKDKPTTSASLSGQFDNSIDTHELLKPFQFTTRSIYDIGLNLTSNQDLVFSPDDYSL
                     KSVLLFYPLEERKFLKNFAFYGRMNLNTHIFEENTFFSENKNIMLQNSAGDTISLLLN
                     QSELKTKIAFYPLRLKEGIGLTYQINFSSNARTSLRVGYGWAQDYNKNSYVFDKTVTS
                     SLDGVNYEFERYKEEPNSSSKGIESTVILSALNLLKFVSINSTLDVLFRMGEPDHSYS
                     LENENRINFRLFRNISLDVKFNISYDEAKKPWTVYDYTTFLRLSLFY"
     gene            12499..13575
                     /locus_tag="PLW08_05800"
     CDS             12499..13575
                     /locus_tag="PLW08_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HPL52150.1"
                     /translation="MSCKIVNNILYLEGSLNAQTVPETFIEVNSLLKQKSPNTIDLSN
                     VNVLDSAGVAFLDELNTRLSAEGILLFQGATPEIQEVIDSFSSLKIKVPAPERKMGFF
                     EKIGDSLVLAGQNFYNVMLLASEVFYWAIIGIFSRKGQRKGSFTIQCSLLGSQALPIL
                     SLLSFIVGFILSLQASVQLANFGANVFIADLMAFAMVREIAPLITAIIVAGRSGSAIA
                     SEIATMQVTEEIDALKTMALSPVRYVVVPKFHAITVVMPILVMFSILVSEIGGGLIAI
                     DYLDLSVYTFVQRSINVLTLKDLVTSFGKSVIFAWTIVIIGAYCGFQVKGGAEGVGKA
                     TTSSVVASIFAVIIWDAIFSLLYL"
     gene            13578..14315
                     /locus_tag="PLW08_05805"
     CDS             13578..14315
                     /locus_tag="PLW08_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL52151.1"
                     /translation="MEPVISIRNLIAKYGDLTVLDDISVDIFPGEITVILGSSGCGKT
                     TLLKNILRLVEPVSGSVKFWGEEILDLDDIQMEKILRKLGVLFQSGALLNSISVYENI
                     SIPLELHTKLPRPLIDRIIQVKLNLVNLNEALYKYPSELSGGMKKRAALARAIALDPQ
                     ILFCDEPSAGLDPLTAASLDELILSLKKQLKMTIVVVTHELASIHRIADKIIFLEEGK
                     MLFNGTLEEAKKAGIPQIDTFFEVGRF"
BASE COUNT         4666 a         2342 c         2955 g         4401 t
ORIGIN      
        1 tttacggaaa tcctgttaga taacattaaa aataatcaga ccaattttat ctatgacctg
       61 aatgaatttt tagagcgcta taaaaaggat aataaagtag aagaaataaa tgattttact
      121 gaagatgatt tgaataagat tgccttctgg atggcaaccg gttcgggaaa aaccttaatt
      181 atgcacatca attattatca gtttttgcat tataaacttt tttctcccga tagcattctg
      241 ttgataactc ccaatgaagg tctttccaaa cagcattatg aagaattaac aaaaagcgga
      301 attccctgtc gtctgtattc cgaaaatttt cgtttttcag atagcgtaaa aaccgaaggt
      361 gaagttctgg ttattgagat gaccaaattg gtggaaacca aaaaaggagg aggtgtaact
      421 atctccataa atgcttttga gggaagaaaa ctgatattcg tagatgaagg acataaaggg
      481 aaaaaagcag aagaacaaaa atgggcaaaa ttgagagata gcctagctga aaacggaatg
      541 gtttttgaat atagcgctac cttcggacaa atactaagcg agaaaaataa ggttacctta
      601 caggaatatg ccaaggcaat tatttttgat tattcctata aatactttta tctggatggc
      661 tatggcaagg atttttccgt gataaacgcg aaaaatatta aagtagacga aaccagattc
      721 caggaaacaa tgtttttagc caatttactt gatttctatg agcaatcgct tatcttcgcg
      781 gatagaaaag ggctggcgga aaaacaaaac ctggaaaaac ccctgtggat ttttgtggga
      841 acaacggtta ccggtagtga agaagaatct gatgtagttc aaattgtcaa ttttttacat
      901 aaagtaatca aagaccccgc ttgggtaaat agcaagctga cagctatttt aacaggaaaa
      961 gcggaattta aaaatgaaag cggagaagac atctttgcta ataagttcac atatataaaa
     1021 gagaaaggac tgaatataga ggatttattc actaaaattt ttggcgggaa aggcggtttt
     1081 ggtatttacg aattgaaaag cgctaaaggt gaatttggct taaaaaccgg agaaaatcaa
     1141 tattttggcg ttatcaatat cggtgatgtt tccagtttta aaaagcaact ggaaaaaata
     1201 ggactaccag tagaaacgga cgctatttcg gaatcgctgt ttgatcatat taaagcacca
     1261 aattcttcag ttaatattct tatcggctcc aagaaattta ttgagggctg ggatacttgg
     1321 agagtatcct ctatgggctt attaaatata ggaacaggac aaggaccgca aattattcaa
     1381 cttttcggta gaggagttcg tttaaaggga caaaatatga ccctgaaacg cagtatggaa
     1441 aaggatataa aacagctgga gaatttgaat atttacggaa tccgggcgga ctatctaagc
     1501 aaatttttag aggtactaag taaagaagaa atggacttgg aaaccattga aatacctgtt
     1561 caacctaata atgtggaaaa atgggaaact ctatatacct tagcaaaaaa agatgtggat
     1621 ttcaataaag aaaaagtttt attcctaaaa gaggagaatt tctccataac ccttgatctg
     1681 atgcctaaat tatccgctta ccttagccaa aaacgcaatg cacaaggtat tgccagttcc
     1741 aatattgcta ttaatacaaa tgaacttaag tcggcaactg atgacgatcc aaggaaaaag
     1801 cttaatctct tttttgattg gaataaaatc tggctggagt tatatgagta taaattggaa
     1861 cggggatact ggaatttgat attgaacaga gattttatta agaattatct gctttattcc
     1921 gataaatata agatattagc tactccggaa actctgcaag ttaactcaat tgaagatata
     1981 ccaaggttgg aaaatatagc tctgcaacta atgaagaaat acattgacca attctataac
     2041 tttcacttaa aaaaatatga gatggaacat ttatactatg cacccgttac cgaagattct
     2101 gctttaagcg tttttgccaa acctgatgac ggatacaatt atactattca aataagcaaa
     2161 aacaaagaaa acaacgaatt gattgcaaat attaaagcat tggtccagga tttggataaa
     2221 ttaatgaaag aagaaaccaa tgaacttccc agaatttact ttgataaaca tctctacctg
     2281 cctatactac tgagagatac aaagatagaa aatatgcatc ccgaagggct ggtggaaagc
     2341 gaaaaagttt ttatttccgg attgaagaat tatctggtat tgaagaaaaa tgaatttacc
     2401 aataaggaaa tatacctgct gaggaatttt cctaaatcgg gcatcggttt tttcaatctt
     2461 agcggtttct atcctgattt tattatgtgg gttaaatatc aaaataaaca gataatttgc
     2521 tttcttgacc cgaaggggtt ggaacatcag aaaagtttgg attcggaaaa agtgcaatta
     2581 cacaaagaaa ttaaagaaat agaaaaacaa cttggaaata aagacattat tctggagtcc
     2641 tttgtccttt ctatcactcc ctatgattca ctgattacag ggttggtaaa tcctgcttcc
     2701 caaaaggaat atgaaaataa tcatattctt tttctgggtt ctatttggtc gggatggcaa
     2761 gatcgattgt ttgatattct ggaaaagagc attagcgcaa ataaaagcga ataaatatac
     2821 cttaaggtgt ttcctctcta ccatttttat tattttaagg ggtgtaaaag accggtaaac
     2881 ttaatttctc tattttcctc tattgcttgt ttaagattgt tttttacaaa ttgcaatcct
     2941 tctgaggtag ggaaaatata agtagtgtag tgattacaga gatgaagttt ttttatctgt
     3001 tcttcctttg gtaaaaattc atcagggtaa tcctcgggct gttcatttag atcgaaactc
     3061 acatttacat atccttcatt tactaaaacc tttaataaat cggatatcgt tccgaaacca
     3121 taattaaaaa cagggtataa agaataaaac aaaattcctt ttcccctttc attaaatatt
     3181 atcataatga ttaacatcaa ttcttcgcag agtgctttgg tcttatcttc cataatttcc
     3241 tccccttttg attaagagtt catagtttct ttttactttt tcccaattta tgaataatat
     3301 tctttgtcaa tctaaattct ggatttatta gccgaaactt gcctaagctt ctcttttttt
     3361 ccagaaagaa taatgccctc atcattctac aaacaacagg gctatgaagt tccctaaaac
     3421 tttttatata gcttaataaa tatattgttg ggtataagaa gaggccaact ttcttgtaag
     3481 tcaggagtca caagcgccta tataattatc aacaaggaaa aagttatcaa gacgcaaatg
     3541 actcctgact taaaggtctt atttattcat tataatggac ttaagtttca agtacttaaa
     3601 tgaaaaatac tgtttcttta caaataaaat atcaaagtaa acttaagcat caagtgctga
     3661 atgacaagta aaagttccat aggaacgaca gatactaacg acggatttca atccggcgaa
     3721 gagaagataa tgataaaaga gttcccgaca gaattacaat tttcttatga tagattttct
     3781 cttttcctgg ctaatgatat tcctctgtat agtaagtatc tatttccttt ctattttgtg
     3841 taattctgac ctttgtcaat tgttttcttt ttttatctat ttctttttgc aggttaaaac
     3901 ccgttaatta tatctgtcgt tcctacggaa ctttttacca tagttatgaa gttgccttgt
     3961 tcaccgggtt caaaacccgt tgttaatatc tgccatccct aacgggattt ttatatatgg
     4021 gttttcaatt tggaaattat attaaatccc tgaaatcaat gtaatcctgt tcatcgcaga
     4081 cctatttttt ctctccgctc taagccctca gccctctgct aaaataatcc tgttcatcgc
     4141 agacctattt tttctctccg ctctaagccc tcagccctct gctaaaataa tcctatttat
     4201 cgcagaccta ttcaatctgc gttatcagcg caatctgcgt gcttatcttt tttttcggtg
     4261 ctttcagtgt aaaaatcctt cttccttttt tgcttgacgg aataaaacag atgtaaagaa
     4321 tagtataaaa gcaaaaatga atattaggat aggtgatgaa ggatacggaa aatatcttgg
     4381 cactcttaaa acaagaggtt tctcctgctt tagggtgtac tgagccggca gctgttgctt
     4441 tagcaggagc ttatgcttca agtgttttaa aggatacaat tatctctgct gatttaattg
     4501 taagccccaa tattttaaaa aacggaatgg gagttgggat tcccaataca aatttaatgg
     4561 gtttggaaat tgcttttgct ttagggatat tagttgccga gccggaaaag ggtctggagg
     4621 ttttattaga tttaaaagcg gaaacaatca acgcgggcaa acagatgctg gcagaaaaaa
     4681 gaattaatac caaaatgaaa gaaacggaag aaaagatatg gattgaagct tctttaaaga
     4741 cggcaaaatc cgaaagcaag gcaattttac gctggcgtca taattggttg tatcattggg
     4801 aaaaagataa gcatattttg caacacaacg aaataggcgc tgaagaagca ccccatttaa
     4861 tatcaccatc tgcttttact ttgaaggatt attacgattt ttgcagggaa acggaaattg
     4921 ataaattgga attaattgat ttgggattga ttttaaaccg tagaatagca gattttggct
     4981 tatccaacaa atccggcatt aatgtaggca agatgattat ggagcaaata aatagcggtc
     5041 ttttgggtga tgacattcat cattatgcaa tggctttaac tgcggctgca actgatgcca
     5101 gaatgagcgg atgcaatttg cctgtgatta gtaattccgg aagcggaaat caggggttgg
     5161 ctattactct gcctataatt gcagttgccg aaaaacttgg cagtaaccaa gaagaactaa
     5221 tcagagcttt agctatgggg catttggtga gtgtgcatat aaaacaaaaa atcggcattc
     5281 tttcctgtat ttgcggatgc ctttctgctt ctgccggagc tgcaagtggc atagttatgc
     5341 ttttaaaagg aacctatcct caaattgaat atgccataaa aaatatgatt gccgatacag
     5401 caggaatggt ttgtgacggt gctaaagaag gttgttcctt aaaagttgcc actactacaa
     5461 gtgctgccgt tcaagctgct cttttagcga aaacaggaat ttgtgtttct tccaatgatg
     5521 gcataattac ggatagcatt gaaggcacta ttgacaattt ggcttatttt gtaaataagg
     5581 gaatgcaaga tgccgacagt actattctaa atattatgtt aaacaagata aaataaagga
     5641 gataaaaaat aatgcctaat agaaaatatg aagtccattt acctgcggaa cttcctccag
     5701 agccaacttt tgaaccaaat attcggagag ctccggacag gggattggat ttaagcaaaa
     5761 aagatatagc cctggcttta aagaatgctc tgcgctatat tcccaaagaa ttacatagtg
     5821 ttctggcacc ggaatttttg caggaactga aaaccagagg tagaatttac ggttatcgct
     5881 atcgtcctca aggtcatatt tacggcaaac cgattgatga atataaaggc attactcctg
     5941 ccagagctat ccaagtgatg atagataaca atctggattt tgatgtggcg ctctatcctt
     6001 atgaactggt tacttatgga gaatcagggc aggtgtgtca gaattggatg caatatagat
     6061 taattaaaca atacctggaa ataatgacgg aaaatcagac attggttgtg gcttcgggac
     6121 atcctttagg gctttttcct tcgcgtccgg aagccccaag agttatatct accaatggtt
     6181 taatggttgg aaaatgggat aacccgattg aatttaaacg cctgacagct ttaggagtgg
     6241 caaattacgg tcaaatgact gctggcggat ggatgtatat aggtcctcag ggaattgtga
     6301 atggaactta tataactttg ctgaatgcag gcagaaaata tttaggcatt ccagaagacc
     6361 aaaacttggc gggggtttta tatgtttcat caggtttggg aggaatgagc ggtgctcaaa
     6421 gtaaagcaat agaaattgca ggtggcattg gaattattgc tgaagtggat aaaagcagaa
     6481 tagaaaccag atataatcag ggttgggtta gtaaggtctc cagtaattta gcagagattt
     6541 ttaactgggt agacgaagcc agacataccc aaaaaccgct ttccattgct tattatggta
     6601 acatagttga tttactggaa tacatagatc agcataacat aaaagtggaa ttgctttccg
     6661 atcagacatc ctgtcatgct gtatatgaag gtggttacac tcctgtagga attacttatg
     6721 aagaagggcg taaacttttg gctgaaaata ttgaattatt caagcaaaaa gtggatgctt
     6781 cactgcgcag gcattttaat gtgctgaaaa atttaactgc taagggctct aaattttggg
     6841 attatggcaa cagctttatg gcaagcgtat ttgaatcggg gataacagaa attgcccgca
     6901 acggaataaa taccaacgag ggttttattt ggccatctta tgtagaagat attatgggtc
     6961 ctatctgttt tgattatggt tacggacctt ttcgctggat ttgtctttcc cgaaaagatg
     7021 aagacctgca taaaacagac ctggcagcta tttctttgat agaccccaat cgtagtgcta
     7081 tggatagaga taattactat tggatttcca ctgcggaaga gaataaactg gtggtgggaa
     7141 ctaaagccag aattctttat gcagatgaag aaagcagaat taaacttggg ctgaaattta
     7201 acgaaatggt tcgtaagggc gaaattggtc ctgttatgtt gggaagagat catcacgatg
     7261 tttcagggac ggattctcct ttccgcgaaa cagccaatat ttatgacggc tcaaatttaa
     7321 tggcagaaat ggctactcat tgctttgccg gaaatattgc cagaggaatg actatggtag
     7381 ttctttccaa tggaggtgga gttggagtta gccgttgtat caatggtgga aatggaattg
     7441 ttttggatgg cagcgaaaga atggatgaag ttgtaaaaag tggtctttcc tgggatgtta
     7501 tgggaggtat tgcacgccga gcttgggctc aaaatgaagg tgcaattaaa actggaactg
     7561 cctggaacga aaaacacagt gctgaaggca atataacgct ggcggaaaag gtggacgaag
     7621 aaatggtaaa atatctcgta aataaggaat ttggagcata aatatgagca ctccctttat
     7681 ccggctgatg aaacaggaag gcataaggga tgaggttatc cggacttttg aggcatatta
     7741 tcagaaactt gttaagggtg aaaaagggct tattggtgaa gatgcaataa gaccaccttc
     7801 ggaaaataat ctaattgatt atcaacaaat aaaacatcta tcccgaactt caatattgaa
     7861 aaatacagtt gtaattaagc ttaatggtgg cttgggaaca agtatggggc tttctaaagc
     7921 gaaatcactt ttgcctgtga agaataatct aaactttctg gatattatca gtcggcaggt
     7981 tcttacttta aggtcacttt caggatatga tgtactcctg ttgtttatga atagtttcaa
     8041 taccgaagcc gatactttaa aatacctgga aaaatatcct gatttaggta aacaggattt
     8101 gcctctttct tttttgcaga ataaatatcc ccgtattcgt caggataacc ttatgcctta
     8161 cgaaaataaa gagagaaaat taatgtggaa tcctccgggg catggcgata tttatacagc
     8221 tttaggggat ttactggata aaatgattgc caaggactac cggtatgctt ttgtttctaa
     8281 tgccgataac ctgggagctg ttgtagatac atcaattcct gcttatatgg aaaataacaa
     8341 tattcctttc gtaatggagg tctgtttacg cagtcctatg gataaaaaag gcggtcacct
     8401 ttgtgaagat aaatcgggac aattgctttt gagagaaatt gctcaatgtc ctgaagagga
     8461 tttacctcgc tttcaggata ttgactatta taaatatttc aacaccaaca atatctggat
     8521 tgacctccga gccctggaat ggcaaatgat tgccaatgag ggtttgatgt tgttgccgtt
     8581 aatagttaat cctaaagttg tagatggaac tccggtttat caattggaaa cagcaatggg
     8641 ttcagccatt agtgtgttca ataattctaa agcattagta gtaagtcggg agcgttttgt
     8701 gccggtgaaa aaaacaaatg aacttttagc tctttggtct gatgcttatg aactgaatga
     8761 acaatatcag attgttttga aaagaggcgt agaaaaggca cctgtaattg aactggatga
     8821 gaaatattac ggtaaaatag aagatttgca aaaaagattc agcaaaggta ttccttcttt
     8881 aagcgaatgc aaagaactaa aaatctatgg cgatgtaagc ttcggggagg atgttatctg
     8941 tgaaggaaaa gtaactctga aagccagttc tcccgtgttt gttcaaaact gtttacttag
     9001 tggagaggtg aatttggatt ctacaattaa taaatctaaa gaccggacag gaggataaaa
     9061 tggtttccca agccgctaaa atccgtctgg gtattttttt agtcattgga gctatcctaa
     9121 taatactttt tgccatagtt gtggctggta gcagattagt ggaaaagaaa gatatctatt
     9181 atattgaatt tgaggactat tctgtaagcg gtttgcaagt tggcagttcg gttaattatc
     9241 gcggaataaa aattggcagg gtggaagatg ttaaaattaa ccccaaggat gtaaccaaaa
     9301 taattattac tatcagcgtg aacagaggaa ctcctataaa agaggatacc gaagcggttc
     9361 ttttgttatc cggaattacc ggactgaaaa atgttgagct aaggggtgga actaatgaag
     9421 caaagttact aaaacccaaa agttacatta aaaccggaac aacggtattg gaagatatta
     9481 gcgaaagggc aaaatctatt gtagataaaa ttgatatgat tgctgccaac ttgagtgcta
     9541 taaccggtga ggaaaacaga aacaatattt ctaccatact tgctcaaaca agtttaatat
     9601 taaaagacac ccatgaaaat ctggctacaa ctatgcaaag tattagtcgc atagctaata
     9661 atactgcgga tttaacagaa gaactaagca aagacctaag cgcggttaca gaaaatctta
     9721 ccaaaaatct tgatgccatc accagcagtg caacaaccaa tattcaaaat gtttccgaga
     9781 cctcacaggc aagtttgatt agtgttacca ataatgtaaa tcaacaactg gaggaactaa
     9841 ctgcaaaact ggaaacaagc ttaactcaat tgaccaatga tggttctgcc ttaattcgcg
     9901 aggcaaattt gaaggtcacc accgttggtg aacatagtga tcaaatgatc ttagaggcaa
     9961 cacgcgaaat attaactatc agcaataata tcaatagagc attggatcag gttaatgcca
    10021 ttctttatac tccaggtttt gacagcttaa tgactaatct gggaaccctt tgtggtgaac
    10081 tttccaaaac tgatttgaaa gggatggtca ctgaactttc cacaactatc caaaaaaccg
    10141 gaactctggt ttccaacctt aatagagttg taactcgcgg acaaggtgac ctgctggaaa
    10201 ttttaagtac tttaaccgag acgagtgaaa acctgaatga attttctcgt cagattgcag
    10261 atacacctgc tattttacta cgcggaaatt aggagctata atgcttaaaa gaaaatgtct
    10321 attcatcttt tcaggatttc tggctattct acttttggtt accggctgtt tgcaaaaagt
    10381 agaaagaact cctgtaaact attatgtttt ggaatatcaa ccgggaacag aaaaaccgga
    10441 attgcgccgt actacaaata caggaaaaaa tctggaagtg ctggatactg ttctgaatag
    10501 aacttatgat agaagtcaaa ttgtagtgaa agaaaacttc tataatgtga aatttctgca
    10561 gacggatgtt tgggcaaccc gtttacgtga tgctattccc aatttgattg tccagcgctt
    10621 aaaggcgtat aatatttttg gaacagtttc cagaggggaa cagctggaaa aaaatcccca
    10681 ctattttctg gagacaaagg tttataacat tgaaaagatt gcaggcacag aacccagagc
    10741 ttatttgaat atggaatttg tgttgcggga ttccacttcc gaaagaattg tttttagtca
    10801 taagggtgaa cgcactatgg aacttataga cccttctatg atctatctgg tgcaggcgtt
    10861 taatgaaatg attatggaag agacagacct ctttgctgca aaatgcaatt tatatttcag
    10921 tggcttgcct gttgaaagta aatctttggc tgcttcaact tctccgattg ccaagtatgt
    10981 ttatgaagaa atgatagtta gggaaacaga ttttgacttt ggagagctga tggttattac
    11041 taaaactcaa acagaggagc aaattcgtta cactattgaa gaattggact cgttgaacac
    11101 agtaatctct actgatgaat tgattatggg tgtgccggca ttactaacac ctggtaatta
    11161 tagagtaata attggtgaaa tgggtgattt aattattccc gtaaaaattt taccccgtca
    11221 gagaacagtt gtaaagccaa actggggaga gctaaaaata gttgttatgg atgaatccaa
    11281 agccagagtc cgaatgggct atgatctctg gaaaaaaaat acagacggcg agggctataa
    11341 aatttacagc gggggtatgt tcagtatggg agaagacgaa gttggcgctg tagataaact
    11401 ttggatttta cctccgggtt cctatttagt aaagcttgga ggtggttcct ggagtgattt
    11461 gagggatttt gccactgtta ctttagcgga aggtgaaaaa aagaccctga caatgattgt
    11521 agaccctgct gctgaaggta atgttttaat tggagcaggt gtttttgccg atgaagatat
    11581 aggtttgggt tcaaaacgaa ttcaccgggg agcaattcat ggtaatattt ctttaacttc
    11641 caataataat gtggataaag acaaacctac taccagtgct tctctttccg ggcagtttga
    11701 taacagcatt gatactcatg aattgcttaa gccatttcaa tttactaccc gcagtatcta
    11761 tgacattggc ttaaatttaa cctccaatca ggatttagta tttagtcctg atgattattc
    11821 cctgaaaagt gttcttttgt tctatccgct ggaagagcgc aagtttctta agaattttgc
    11881 tttttatgga agaatgaatt tgaataccca tatatttgag gaaaacacct ttttttcgga
    11941 aaacaaaaat ataatgctgc aaaattctgc gggcgataca atatctctgc ttttgaatca
    12001 gtccgaatta aaaaccaaaa ttgccttcta tcctttacga ctgaaagaag gaatcggatt
    12061 gacctatcaa attaatttca gttccaatgc cagaacaagt ttacgcgttg gctacggttg
    12121 ggctcaggat tataataaaa atagctatgt atttgataaa acggtaacaa gttccttaga
    12181 tggagtaaat tatgaatttg aaaggtataa agaagaaccc aatagcagtt ccaaaggtat
    12241 tgaatccaca gttattcttt ctgccttgaa tctgctcaag tttgtaagca tcaattctac
    12301 tttggatgtt cttttccgga tgggagaacc cgatcattct tacagtttgg aaaacgaaaa
    12361 caggataaat ttcagattgt tcagaaacat ttctttagat gttaaattca atatttctta
    12421 tgatgaagcc aaaaaaccct ggacggtata tgattatacc acattcttgc ggctatctct
    12481 attctattaa gaggcataat gtcctgcaag atagtaaata atattctata tctggaagga
    12541 agtttaaatg cgcaaacagt tccggaaaca ttcattgaag taaattcgct tctaaaacaa
    12601 aaaagtccca ataccattga tttaagtaat gttaatgttt tagatagtgc aggagtggct
    12661 tttctggatg aattaaatac ccgtttatcc gcagaaggga tattgttatt tcaaggcgca
    12721 actcctgaaa tacaagaggt tatagatagt ttttcctccc tgaaaattaa ggttcctgct
    12781 ccggaaagaa agatgggttt ttttgaaaaa ataggggatt ctcttgtctt ggcaggacaa
    12841 aatttctata atgtaatgtt attggcttcg gaggttttct actgggcaat tataggtatt
    12901 ttcagcagaa agggtcagcg taaaggttct ttcaccattc aatgttcttt attgggttcg
    12961 caggcattgc ctattttatc tttgctttct tttatcgtgg gttttatact ttccttgcag
    13021 gcatcggtgc aattggctaa ttttggtgct aatgttttta tagcagacct gatggcattt
    13081 gctatggtgc gggaaattgc tcctttaatt acggcaatta ttgttgccgg aagaagtgga
    13141 tcagccattg cttccgaaat tgccacaatg caagttacag aagaaattga tgctttaaaa
    13201 actatggctt tgtcccctgt ccgctatgta gttgttccca agtttcatgc aattactgtt
    13261 gtgatgccga ttctggtgat gttttccatt ttggtttcgg aaattggagg tggacttata
    13321 gccattgatt atttggattt gagcgtttat acttttgttc aacgctccat taatgttctt
    13381 accctgaaag atttggtaac cagttttggc aagagtgtta tttttgcctg gacaattgtt
    13441 ataattggtg cttattgcgg ttttcaagtt aagggcggag cagagggtgt tggcaaggca
    13501 actacttctt ctgttgttgc ttccatcttt gctgtcatta tttgggatgc tattttcagc
    13561 ttactctatt tataattatg gaaccagtaa tcagcattag aaatttgatt gccaaatatg
    13621 gcgatctcac tgtgttggat gatattagcg tggatatttt tcctggggag ataaccgtta
    13681 ttttgggaag tagcggttgt ggaaaaacaa ctcttttaaa aaatatttta cgcctggtag
    13741 agccggtttc cggaagcgtt aaattctggg gtgaagaaat attagacctt gatgatattc
    13801 aaatggaaaa gattttgcgt aaattggggg tcttattcca aagcggtgcc ttgctcaatt
    13861 ccatttccgt ttacgaaaat atcagtattc ctctggaatt gcatacaaaa ctgccccgac
    13921 ctcttattga tagaataatt caagtaaaat taaatctggt aaacttaaac gaagcactgt
    13981 ataaatatcc ttccgaactt tccgggggaa tgaaaaaaag agctgcctta gcccgagcta
    14041 ttgctcttga tcctcaaatc cttttttgtg atgaaccttc tgcaggactt gatccattaa
    14101 ctgccgcttc tttggatgaa cttattttaa gcttaaaaaa acaattaaaa atgaccattg
    14161 tagttgttac tcatgaactg gcttctattc atagaattgc cgataaaatt atctttctgg
    14221 aagagggtaa gatgcttttt aacggaactt tggaagaagc caaaaaagct ggaataccgc
    14281 aaattgatac cttctttgaa gtaggcagat tctgagtatt ataattattg ctgcttatga
    14341 tctgaaccat ctaattggta aaaa
//
LOCUS       DAOOZV010000048        13851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4434_length_13851_cov_66.369455, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000048 DAOOZV010000000
VERSION     DAOOZV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13851
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_13851_cov_66.369455"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1848)
                     /locus_tag="PLW08_05810"
     CDS             complement(118..1848)
                     /locus_tag="PLW08_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52152.1"
                     /translation="MRKTFLVLILVSGIVFCFAQEFNSFRYLSTSGALDGDVEYAFDP
                     LDLNYLSGTRFFSGLSNFSGNDKILMNSGDNYLLLGTSTDKTFLKKLKAALLFKYYDQ
                     EDPLEINCYPSPYDVYPTTDWGRVEYTWQNYYDTNGNSLYDRYGYVHQKYENVAKDKG
                     TDFFFVLSHQLGEKSVLGYKLGFLYSQESNTYAQDNILSALAGDPTWEYTYNIINLPE
                     IDPTIAEETISSNYLGDFDTKDTNSTLRNQLGYLTKLNAWEFSGTYTLDYLDNCLETK
                     DFASQIYSYNSDITNYYHAYRKNNYKENGMFNQISGRVRYILLPDDDPLHTGSVSCGL
                     GFGLRTLNVERKELYNEDKDNNDSCYHNIISENLSSSGDLTGINFSANTRLNYPLNNK
                     TFIGTGLFYNLQIDDLEGDFTYNVTRLDSIFNYDGSWNENINIISKSAGKVKQEETVR
                     IFRAPVGLEYWFTNNQKWAMRFGTVFTHKVEIYKESYAPTSIEPTIIEETYANGETYT
                     NIDNNISLKESHCTKYSTSKTDFSYGICFKANNNLKIDLLTMFDNEDLELWNTDFLRN
                     LKLSFTVIFK"
     gene            complement(1845..3206)
                     /locus_tag="PLW08_05815"
     CDS             complement(1845..3206)
                     /locus_tag="PLW08_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52153.1"
                     /translation="MRITTTFLWVLLCFLLFLTACDEDLLKPADYNPDNANRKPLFHR
                     SLLMDETGDCYTGYRNFNIRPGKVKLEWDTSNDNNFLLYRLYRENSLITTIQDRSVNT
                     YTDSLLTPNTKYHYTIVTQVRTGLSKSDTLSVKTASNLPPLINYRVNNNFEVILYWKD
                     RSDIPGKFQIFKNDEPIGEVNEILPTDYEHIYSFKDTATNPYYYYTYQIKKVGVQDST
                     SLSSPLGVYVYYSMIPLTLNSLHQIPGTTNVVLDWTDTCTGETGFRIYRKESSGTNFS
                     RIKTINESNVTQYTDTDPLVLGKTYDYYVTAIDAEISPVIETAPSNILSITIVENMQV
                     EWQIALKDSYGDGWNGGYIKLYINGTPIFDYLTLSNGAGPEYYTFTVTEGDQIDIYYY
                     PGNWSNENYYAILDQNGNIVAESGGTWNDPGVTTPGNLSITVDLGGGKAGNKAVAQIG
                     GEK"
     gene            3890..6463
                     /locus_tag="PLW08_05820"
     CDS             3890..6463
                     /locus_tag="PLW08_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL52154.1"
                     /translation="MIVRYEDKKDLGKKEEVKQQKQQIEDFIRAFKQEKRFASTIVAE
                     NTEPAKAAQTDFYPEGLLPPVQNLLQRMGIEKPYIHQAEAIKALLAGKNVVVSAGVAS
                     GKSLVYQAVILNSLVQNPQSRALLLFPTKALAQDQAQKMQMLCKELNKQNPALPAIVN
                     GIYDGDTATDLRGKLRKQANILFTNPDMLHLGILPNHTLWSAFFSRLAYVIIDEVHIY
                     RGVFGSHTANLFRRLKRITAVYGIKPQFIFTSATLANARELAETLIEEPVVLIDKDGS
                     PKGERHFYICNPPVIEPALGIRRSSTQEISSIAKSLFQTNLQALLFTGSRRSVELIFR
                     YLTADRKIADKVRSYRSGYLAEERRKVERDLREGEIGLVISTNALELGIDIGGLDAIL
                     LNGYPGTICATRQQAGRAGRKGNPSLCILEASGNPLDQYICQHPEYIFANNPEQALLD
                     PNNSEILRLQLLCAISEMAFKEGENFGALSFAEIQGHLFALLDEGLIRHIGNRYIGIS
                     GKYPAGDVSLRNAGNQFQILADDELVGWVDSGSVKWMTHPNAIYLHQGETWVVKELSI
                     EQKKVILEPVQVNYYTQATQFTEIALNNLLRLENVTGGRKHFGEVTVTRTITGFKRLR
                     FWTMEVLDQEELDLPPEIMQTQAYWISLAEETVERIRNQGLWNNDRNDYGTKWEEICE
                     QIRQRDNYHCRNCGATGDLEVHHIIPFRRFEDPEEANEPDNLVALCPRCHRLAETRVH
                     IQSGLAALAYLLGNLAPFFVMCAPQDLGVHSEDKSPLALGNPVIVIYDNFPGGIGLSR
                     KLYELHNQLLYAGMDRIKDCACENGCPACVGPVAENGIGAKEEAMAILKELIEEGRIE
                     N"
     gene            6699..8909
                     /locus_tag="PLW08_05825"
     CDS             6699..8909
                     /locus_tag="PLW08_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPL52155.1"
                     /translation="MKKTLLVIVLLLTLSLLLALPAEFTRAGNPELYKELSAKAKNNL
                     KHLTLEQKQQYKDLLKQYPDILMNYLLAYESDSNLAEAKPEDVKSNYEMICALLNKKG
                     LKYPVDFFLSYIAKQTVSDEPLTAYRKILLQDGLQNIINTTNTDLEMFRAVTLWCVSR
                     IQFQQTSGRDQSPLDIVQKSLIGRCEEMQILLVSACRTIGLPARPAYTPWWAHTDNNH
                     AWTEIYLDGEWHYTGDMDMAWFPDNTWFSGLVDKTVLILADGTLTTWKDEVLIKGKYD
                     AVINSTANYTKDRSRKVKIKVVDSNNKPIPNANVLPMVFNWGSLRSLINLTTDKNGYL
                     EFTTGRGAFYLSVYSKEGKALVLIPSTAEKTIEKKITVTNTDFAGGWATLEYPENSVE
                     SKTQPEEYIIAVNLEKERWQEKQKTLENEALKTKTTDDSLAIKVALACRGNYSAFWEF
                     YKRNPNPDSNFLQFLLEGDPKLLWQANAELLEALYNNWLKLYKPEMDEEKVMSLFSPV
                     VYYENLPQVVNYKKGKAQLYPESFRYNYPHNRDGVIKVLSRLKQKYKINSKKALSGLP
                     PLHILVQHKNLTAVQYKIMAVCFLRANGYPSEYSRIPNLIAVFIDGDWQYVNVVKCAW
                     EDLSQDNKTNTFQLILQIKDDKGNPIEAKEEQLTLCRYAEGAFYPLNDNFAYLGSGKY
                     NGVFPVSDVWLQFGYRVSEKQTRVFLQPLKPKVDDTLSLEIVAEDYPFEKKEEN"
     gene            8968..9771
                     /gene="mutM"
                     /locus_tag="PLW08_05830"
     CDS             8968..9771
                     /gene="mutM"
                     /locus_tag="PLW08_05830"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPL52156.1"
                     /translation="MPELPEVQSIINDLEKVLKGKTIKTIECYYSGTVITNCLPEDNP
                     FPIRALAIKRRGKYIIIMLEKDNALIIHLRMTGKLIYDNFCGEPLKYERARIILENNE
                     VLHFIDIRTFGKIVICSQKNIEHCLPPLGLEPFSDDFTAKVLKEKMHGKKTPVKIALM
                     DQKIIAGLGNIYVCEILYRAGINPEKPANKITRKNIVEIIQQTKEVLTEAIDKGGTSI
                     SDYRRIDDKRGSFQNFLQVYQKQFCPLGHKVLRLKQGGRSTFYCPICQK"
     gene            9973..11169
                     /locus_tag="PLW08_05835"
     CDS             9973..11169
                     /locus_tag="PLW08_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPL52157.1"
                     /translation="MKAIKLKEGVYWVGVIDWDLRNFHGYITQRGSTYNAYLIIDEKV
                     TLIDNVKSYLFEEMLSRISDIIDPSKIDVLIQNHTEMDHSGGLPELIKYLPNTSIYTN
                     ANGIKALKRHFKQDWNFNEIKSGDSIKIGKRTLTFLTTPMVHWPDNQFTYCPEEKILF
                     SNDGFGQHIASNERFAKELPAGIVLEEAKKYYANIVLPYSKQVQKVLEEAGKWDINMI
                     CPSHGVIWTAENIPEILSAYHNWAYNIADDYKALIIYDTMWKSTQKLAYTISAAFEAE
                     GIRAQLVNLQTNHISDIMTAVMDAKFIAVGSPTLNSSILPTVAAFLYYLKGLSPRNKI
                     GLAFGSYGWGGQSIPILHQLLGDPKECGFAMLEPVQVQYIPDEEELNRIKSNLQRQIR
                     EKLNAM"
     gene            11238..12203
                     /locus_tag="PLW08_05840"
     CDS             11238..12203
                     /locus_tag="PLW08_05840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPL52158.1"
                     /translation="MKSLPQKDLYAKIFNSALVAIGTTDTKGNFIAVNPAWCKALGWT
                     EEETNTLNLKDITLAEEQQTLNQELSKLAEGKVCSLRKTQRYKRKDGSFFWADLYRSA
                     LFDDKGKVMGILDIFVDIDVQVRSEKVQGELFQSLETLNKELSNANIGLKRQAKYDSL
                     TGLYNRWVMEEMLNKEIISSLATNRGFAVGIADIDDFKKVNDTYGHDCGDLVLVKLTQ
                     SFLQKIRVTDSVCRWGGEEFLFLFSETTESGAMIVVERIRKAIEELTINYKGNQIKVT
                     ITIGVSFYNGQDHLDSKELVDRADKAMYEGKSSGKNKAVLYSNIP"
     gene            12352..12927
                     /locus_tag="PLW08_05845"
     CDS             12352..12927
                     /locus_tag="PLW08_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52159.1"
                     /translation="MKTNSRSWQPTLTLARLFEEQNQFYDALAAYELISQNDPSPELR
                     ERIEDLHLRILNDPNRTYDPRLEQLFSPEELAYLKILDHQGFLNMAKAREKLMEEMQG
                     SELIFSEVMPEDVLVDESELDTLREILEEIEQQAQLSLISETGDVGEYNVQDFLIAML
                     AKFDKDQKLSEVKLSDLLSLFLEMQSLKNQP"
     gene            12927..13094
                     /locus_tag="PLW08_05850"
     CDS             12927..13094
                     /locus_tag="PLW08_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52160.1"
                     /translation="MKQKHTLNINSLCASCYEKCKQKDLVLIITCPFYDPFPVQLELK
                     FKGLRKKPKNK"
     gene            13180..13752
                     /gene="rsmD"
                     /locus_tag="PLW08_05855"
     CDS             13180..13752
                     /gene="rsmD"
                     /locus_tag="PLW08_05855"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPL52161.1"
                     /translation="MRIITGIYKKRNLFLVPGMSTRPTSSFNREVIFSVLQDYAGFRV
                     LDLFAGSGSLGLETLSRGAVWVDFVEFAPSAISTILQNINLLGCSENCHLWRKKVDVY
                     LKSCENKYDIIFIDPPYNKNLINPTLKLIYEKSLLNEEGSIIVEHSPQEIIAEEFLPY
                     ITKEKYFKRSHFTWLRARNNDLNQESSLPK"
BASE COUNT         4537 a         2408 c         2795 g         4111 t
ORIGIN      
        1 tcaaccatcc ggaaaaaaac atctattaac tacacaaaaa gcggaggaac agcgtcctcc
       61 gtctacataa aaaagggagc gggaaaaaac cgctcccaga agtgagaagg atttagttta
      121 tttgaagata acggtaaagg acaatttcaa gttcctgagg aaatcagtat tccagagttc
      181 cagatcttca ttatcaaaca tagtcagcag gtcaattttt aggttattat tagctttaaa
      241 gcaaattccg taactgaaat ccgttttgga agtagaatat tttgtgcaat ggctttcttt
      301 tagggaaata ttattatcaa tattggtgta agtttctcca tttgcatagg tttcttcaat
      361 tatggttggc tcaattgagg taggagcata actttcctta tatatttcta ccttatgtgt
      421 gaaaacagtg ccaaagcgca tagcccattt ctgattatta gtaaaccagt attccagtcc
      481 tacgggagca cggaaaattc taactgtttc ttcttgtttc actttacctg cagatttaga
      541 tattatatta atattctcgt tccagcttcc atcataattg aaaatagaat ccaatctggt
      601 aacattatag gtaaaatcgc cttccaagtc atcaatttgc agattataga ataaacctgt
      661 gccaatgaag gtcttattat taagaggata gtttagccgg gtattagctg agaagttaat
      721 acctgttaaa tcgcctgagg aagaaaggtt ttcgctaatg atattgtgat agcaagagtc
      781 attgttatct ttatcctcat tgtataattc ctttctttcc acattcaaag tccgtaatcc
      841 aaagccgaga ccacagctga cagagccagt atgtaaagga tcatcatcag gaagcaaaat
      901 ataacgcaca cgaccggaaa tttgattaaa cataccattt tctttataat tattttttct
      961 gtaggcatgg taataatttg taatatcgga attatagcta tatatttggg aagcaaaatc
     1021 cttcgtttcc aggcaattat ctaaataatc caaagtataa gttccggaaa attcccaggc
     1081 atttaattta gtcaaatatc ccaattgatt acggagagtg gaattagtat ctttcgtatc
     1141 aaaatcaccc aaataatttg aagagatagt ttcttccgct atagtagggt ctatttctgg
     1201 aaggttaata atattatatg tatattccca agtaggatcg cccgcaagtg cggaaagaat
     1261 attatcctga gcataagtat tactttcttg ggaataaaga aagcccagtt tgtagcccag
     1321 aacactcttt tcacctaact gatgcgaaag gacaaaaaag aaatcagttc ccttatcttt
     1381 agccacattt tcatatttct ggtgaacata cccataacgg tcatagagcg aattgccatt
     1441 cgtatcgtaa taattttgcc aggtatattc cactctgccc caatcagttg taggatatac
     1501 atcataagga ctgggataac aatttatctc cagggggtct tcctgatcat aatatttaaa
     1561 gagaagagca gctttaagtt tttttagaaa tgttttatcc gtagaggttc ccaagagaag
     1621 gtagttatct ccactattca tcaaaatttt atcattaccg ctgaaattgc ttaatccgct
     1681 gaagaaccgt gttccactta aataattaag atcaagagga tcaaaagcat attctacatc
     1741 accgtccaaa gcaccgctgg tagaaagata ccgaaaggaa ttgaattcct gggcaaagca
     1801 aaacactata ccgcttacga gaattagaac taagaatgtc ttcctcattt ttcacctcct
     1861 atttgggcaa cagctttgtt tccggctttg cctcctccta aatcaacagt tatggataaa
     1921 ttaccagggg tagttacccc aggatcattc caagttccac ctgattcagc tacaatattg
     1981 ccattctgat ctaatatagc ataataattt tcattagacc aattaccagg ataataataa
     2041 atatctatct ggtcaccttc tgttactgta aaagtataat attccggtcc ggctccatta
     2101 gacaaagtta agtagtcaaa tataggagta ccattgatat atagttttat atatcctcca
     2161 ttccatccat ctccgtaaga atccttaagg gctatttgcc attcgacttg catattttca
     2221 acaattgtta tagaaagaat attagaggga gctgtttcaa ttacgggact aatctctgca
     2281 tctatagccg ttacataata atcataggtt ttcccaagca ccagaggatc agtatcagta
     2341 tattgagtta cattgctttc gttaatagtt ttaatccgtg aaaaattagt tccacttgat
     2401 tctttcctgt agattctaaa accagtttca ccagtacaag tatccgtcca atccaaaacg
     2461 acattagtag ttccaggtat ttgatgaaga gaattcaaag tgagaggaat catagagtaa
     2521 tatacataaa cacctaaagg agaagataga gaagttgaat cttgaacacc tactttttta
     2581 atttgataag tataataata gtatgggtta gtagcagtat ctttgaaaga gtagatatgt
     2641 tcataatcgg ttggcaggat ttcattaact tcaccaattg gttcatcatt cttaaatatt
     2701 tgaaacttgc cagggatatc gcttctatct ttccagtaaa gaataacttc aaaattattg
     2761 tttacccggt aattaattaa aggtggtaga ttggaagcgg tttttacact taaagtgtct
     2821 gatttactta agccagttct cacttgagtg actatagtat aatgatattt agtattagga
     2881 gttagaagag aatcagtata agtattaaca gaacgatctt ggatagtagt tattaatgaa
     2941 ttttcccggt ataaacgata gagcaggaaa ttattatcat ttgatgtatc ccattccagt
     3001 ttaactttac caggacggat attgaaatta cgatagcccg tataacaatc accagtttca
     3061 tccattaata gtgaacgatg aaacaagggt ttgcgattgg cattatccgg attataatct
     3121 gctggtttta gcaaatcttc atcgcaagca gttaaaaaca acaaaaaaca tagtaacacc
     3181 caaagaaatg ttgttgttat tcgcatttta tcctcttgta aattttttgc aactatatat
     3241 agcataaata ttctgtcaag tttattttaa agtgtaggca gaggatgccg ttcctcagca
     3301 tttttttagc taccgaaatc accgggaaaa acgaggacaa caagattttt ttctattttc
     3361 taagaccatt tctggaggga aaaagcattt atcattcctg cgaatgaaag aatccacatc
     3421 agggataaaa tatttatgca ttttcagtgg tttaaacaca catcgcatga agcagagagc
     3481 ggagggcgga gggcagagag aatctcaaca atctaaacca tttttctggt agaggaacgg
     3541 tgtcctccgt ctacagcttt gcaggaagca ttgcttactc aatacggact cattgctaat
     3601 gccatccgta atgggtaagc aatgggtaag caatgctatt agggaagaca ggagaaaacg
     3661 gtataacagc ttataataca aatagataga tgtttatacc aaaataaggc aataaaaatg
     3721 aataatacac aaattgagaa attgggttgc caaattccaa gttggaaaat gctgaaagca
     3781 aatttcttga gaaacttccg gttgctttag ccaagaaaga atttaccaac tctttttcct
     3841 tccgccgcat tttttaggtt aaagataata cttgaccgta actaccataa tgatagtgag
     3901 gtatgaagat aaaaaggact tggggaaaaa ggaagaagtg aaacagcaaa agcaacaaat
     3961 agaggacttt attcgggctt ttaagcagga aaagcgtttt gcctctacaa ttgtagcgga
     4021 aaatacagaa ccggctaagg cagcgcaaac ggatttttat cctgaaggtc ttttacctcc
     4081 tgtgcagaat ttactgcaaa ggatgggcat agaaaagccc tatattcatc aggcagaggc
     4141 aattaaagct cttttagcgg ggaaaaatgt agtggttagt gccggggttg ccagcgggaa
     4201 aagtttagtt tatcaagcag ttatcttaaa cagtttggtg caaaatccgc agagtagagc
     4261 tttgcttctt tttccgacaa aggcattggc acaggatcag gcgcagaaaa tgcagatgct
     4321 ttgtaaagag ctgaacaaac aaaatcctgc gttaccagca attgtcaatg gcatttacga
     4381 tggcgatacg gcaacggatt tacgcggaaa actacgcaaa caggcaaata ttctttttac
     4441 taatcccgat atgttgcatt tgggaatttt gccgaatcat actttatgga gcgcattttt
     4501 ctcccgttta gcttatgtga taattgatga agtgcatatt tatcgtggtg ttttcggttc
     4561 gcatacggca aatttgttca gacgtttgaa acgcatcacg gctgtttatg gcataaaacc
     4621 gcagtttatt ttcacttccg ctactttagc caatgcgcgt gaacttgccg aaacccttat
     4681 tgaagaaccg gttgtgctga tagataaaga tggttcaccc aaaggagaaa ggcattttta
     4741 tatttgcaat ccaccagtta ttgagcccgc tttaggcatt cgcagaagtt ctacccagga
     4801 aataagcagt attgctaaaa gtttatttca aacgaattta caggcacttc tttttacggg
     4861 cagccgacgc agtgtggaac tcatttttcg ttatttaacc gctgatcgga aaatagcgga
     4921 taaagttcgc agttacagaa gcggttattt ggctgaagaa cgccgaaaag tagaaaggga
     4981 cttacgggaa ggagaaatcg gtttagttat ttctaccaac gccttagagc tgggaattga
     5041 tatcggagga ttggatgcta tccttttaaa tggctatccc ggaacaattt gtgccaccag
     5101 acaacaagcc ggtagagctg gaagaaaggg aaatccatct ctttgtattt tggaagccag
     5161 cggaaatcct ttagaccaat atatctgcca gcaccctgaa tatatttttg ccaacaatcc
     5221 tgaacaggca ttattagacc ctaataacag cgaaattttg cgtctacaac tgctttgtgc
     5281 catcagtgaa atggctttta aggagggaga aaacttcggt gccttaagtt ttgccgaaat
     5341 tcagggtcac ctttttgccc ttttggatga gggcttaata aggcatatcg gaaaccgcta
     5401 tataggaata agcggaaaat atcctgccgg tgatgtttct ttacgcaatg cgggaaatca
     5461 atttcaaatt ctggcagatg acgaattagt tggttgggta gattccggaa gcgtgaaatg
     5521 gatgacacat cccaatgcta tttaccttca tcagggtgaa acctgggtag taaaagaact
     5581 gagtatagaa cagaaaaaag tgattttaga gcccgttcag gtaaattatt acacgcaggc
     5641 aacacaattt actgaaattg ctttaaacaa tctgttgcgt ctggaaaatg ttacaggagg
     5701 acgtaaacac tttggagaag tgacagttac cagaactata accggattta aacgcttgcg
     5761 gttttggaca atggaggttt tagaccaaga ggaacttgac cttccacccg aaattatgca
     5821 gacccaggca tattggattt cccttgcaga agaaacggta gaaagaatac gcaatcaggg
     5881 tttgtggaat aacgatagaa atgattatgg aactaaatgg gaagaaattt gtgaacaaat
     5941 ccgccagcgc gataattatc actgccggaa ttgtggtgca actggggatt tggaagtgca
     6001 tcatataatt ccttttcgcc gttttgaaga ccccgaagag gcaaatgagc cggataatct
     6061 tgtggcttta tgcccacgct gtcaccgttt ggcagaaacc agagtgcata ttcaaagcgg
     6121 attagctgct ttggcatatc tgcttggtaa tttggcacct ttttttgtaa tgtgcgctcc
     6181 tcaggatttg ggagttcata gtgaagataa atctcctctg gcacttggca atcctgtaat
     6241 tgttatttac gataattttc ccgggggaat aggtcttagc cgaaaattgt atgaactgca
     6301 taatcaactt ttgtatgccg gaatggatag aattaaggat tgtgcctgtg aaaatggctg
     6361 tcctgcctgt gtgggtcctg tagcggaaaa tgggatagga gcaaaagaag aagcgatggc
     6421 tatcctaaaa gaactgatag aagaaggaag aattgagaat taaatcagga tttattagca
     6481 ccttcacaaa ttttttcctt atgttagcac gagtccttac tgccaaacga aatcgtggga
     6541 tgttcataat ttttgctggg agtgacgacc cctgacttca atcattctca attctcaatt
     6601 cttcattcta tagctttttc ttgcctgcgt ttaattttgt cttattttat gtataaaaag
     6661 aaaataatgc ccagaaaaaa aagtaaaagg ggccagaggt gaaaaaaaca ttattagtca
     6721 ttgttctact cctcacgctt tcattgttat tagctcttcc tgcagaattt acccgtgctg
     6781 gaaatccgga actttacaaa gaactttctg caaaggcaaa aaacaatttg aagcatttaa
     6841 ctttggagca aaaacagcaa tacaaagacc tgctgaaaca atatcccgat attttgatga
     6901 actatctgct tgcttatgaa agtgacagta atttggcaga agcaaaaccg gaagatgtaa
     6961 aaagcaatta tgaaatgatt tgtgcgctgc tgaataaaaa aggactgaaa tatcctgtgg
     7021 actttttcct ttcctatata gccaaacaaa ccgtttctga tgaacccctt acggcttatc
     7081 ggaaaatatt attgcaagac ggtctgcaga atataatcaa taccaccaat accgatttgg
     7141 aaatgtttcg tgccgtaact ctctggtgtg tttccagaat tcagtttcaa cagacctcgg
     7201 gaagagatca aagtcctttg gatattgtgc aaaagtcctt aatcggacgc tgtgaagaaa
     7261 tgcaaatttt acttgtttcc gcctgccgaa caattggact tcctgcccgt cctgcatata
     7321 caccttggtg ggctcatacg gataataatc atgcttggac ggaaatttat ttggacggag
     7381 aatggcatta taccggagat atggatatgg cttggtttcc tgataatacc tggtttagcg
     7441 gtctggtaga taaaacggtg ctgattttgg ctgacggaac tttaactacc tggaaagatg
     7501 aggttttaat taaaggcaaa tatgatgcgg taatcaattc tacagctaac tatactaaag
     7561 accgcagccg gaaagtgaaa atcaaagttg tggatagtaa taataagcca atacccaatg
     7621 ctaatgttct tccaatggta ttcaactggg gttccttaag gtctttaatt aatttaacta
     7681 ccgataaaaa cggctacctg gaatttacta cgggtagagg tgctttttat ctttcagttt
     7741 acagcaaaga aggtaaagct cttgttctga ttccttccac tgcagaaaaa actatagaaa
     7801 agaaaattac tgttacgaat actgattttg caggtggctg ggcaactttg gaatatccgg
     7861 aaaacagcgt tgaaagtaaa actcaacccg aggaatatat tattgcggtt aatctggaaa
     7921 aagaacgctg gcaggaaaaa caaaagacat tggaaaatga agcgttgaaa acaaaaacta
     7981 cggatgattc cctggcaata aaagttgctc ttgcttgtag aggtaactat tccgctttct
     8041 gggaatttta taagagaaat cccaatcccg acagcaattt tttgcagttt ctgctggaag
     8101 gtgatcccaa acttttatgg caggcaaatg cggaattgct ggaagccctt tataataatt
     8161 ggttaaaact atataaaccg gaaatggatg aagaaaaggt gatgagttta ttctctcctg
     8221 ttgtttatta cgaaaacctg ccacaggtgg taaactataa aaaaggtaaa gcacaactct
     8281 atccggaatc attccgctat aattatcccc ataatcggga cggagttata aaagtgcttt
     8341 cccgcttgaa acaaaaatat aaaataaact ccaaaaaggc attaagcgga cttccccctt
     8401 tgcatatttt agttcagcat aagaacttaa ctgcagtgca gtataaaatt atggcagttt
     8461 gttttttgag ggctaatggc tatccttctg aatattcgcg tattccaaat ttgattgcag
     8521 tgtttattga tggtgactgg caatatgtaa atgtggtaaa atgcgcttgg gaagaccttt
     8581 cccaagataa taaaaccaat acttttcagc ttatcttgca aattaaagat gataagggaa
     8641 atcctattga agctaaagaa gaacaactta ccctttgccg ctatgcagag ggagcgtttt
     8701 atcccttaaa tgataatttt gcatatctgg gctcgggaaa atataatggt gtttttcctg
     8761 tttctgatgt ctggctacag tttggatata gggtttcgga aaaacaaaca agggtcttcc
     8821 tgcaaccctt aaagccaaaa gtggatgata cactatccct ggaaattgtt gcagaggact
     8881 atccttttga gaaaaaggaa gaaaattagc tctcacagat tacacggatt tcccagataa
     8941 aagatgaata ataaaaggag aggataaatg ccggaattgc ctgaagtgca aagcattatc
     9001 aacgatttgg aaaaagtgct gaaaggcaaa actataaaaa caattgaatg ttactattcc
     9061 ggaactgtga ttacgaattg tttgccggaa gataatccct ttccaattag agctttggca
     9121 ataaaacgcc gcgggaaata tattattata atgctggaaa aggataatgc cttaataatt
     9181 cacttgcgga tgacaggtaa actgatttat gataattttt gcggtgaacc tttaaaatac
     9241 gaaagagcca gaataatcct ggaaaataat gaagttctgc attttataga tattcgcact
     9301 ttcggaaaaa tagttatctg cagtcaaaaa aacatagagc attgccttcc gcctttggga
     9361 ctggaaccct ttagtgatga ctttactgct aaagttttaa aagaaaaaat gcacggcaaa
     9421 aaaactcccg taaaaattgc tttaatggac cagaaaatca ttgccggatt gggaaatatc
     9481 tatgtttgtg aaattctcta tcgggctgga attaatcctg aaaaaccggc aaataaaatt
     9541 actcggaaga atattgtgga aattattcaa cagacaaagg aagtccttac ggaagcaata
     9601 gataaaggtg gaacaagtat ttcggattac cgcagaattg atgataaacg gggcagtttt
     9661 caaaacttcc tgcaggttta ccaaaagcag ttctgtcctt tggggcataa ggttttacgg
     9721 ctgaaacaag gtggacgttc tacattctac tgccctatat gccaaaaata aaaattactt
     9781 gacctgcttc tttatctcag atattatata attatgttag ttctatattg aaagttcagt
     9841 tagacagatt aaaaaatatc tggattcctg tttgcacagg aaggacaaaa gaattttcaa
     9901 aagagaaatg attttatata gaagaacgat gaggacatcg ttattccgca taaaaaagaa
     9961 ggtggataag atatgaaagc aattaaactg aaagaaggcg tttactgggt tggagttatt
    10021 gattgggatt tgcgtaattt tcatggttat ataactcaac ggggtagcac ttataatgcc
    10081 tatctaatta tagatgagaa ggttaccctt atagataatg tgaagagtta tctgtttgaa
    10141 gaaatgcttt cccgaataag cgatataatt gatccctcca agattgatgt tttaattcaa
    10201 aatcatacgg aaatggatca tagcggtggc ttaccggaac tgattaaata ccttcccaat
    10261 acgtctatct acactaatgc caatggtata aaagctctga aaaggcattt taagcaggac
    10321 tggaatttta acgaaatcaa aagcggagac agcattaaaa tcgggaaaag gactttaact
    10381 tttttaacta ccccgatggt gcactggcct gataatcaat ttacttattg tcccgaagag
    10441 aaaatcctgt tttccaacga tggtttcggt cagcatattg cctctaatga acgctttgcc
    10501 aaagaacttc cagcaggtat agttctggaa gaggcaaaaa agtattatgc caatattgtt
    10561 ttgccctatt ccaaacaggt tcagaaggtt ttggaagaag ccggaaaatg ggatataaat
    10621 atgatttgtc ccagtcatgg agttatctgg acagcggaaa atattcctga aatcctttct
    10681 gcttaccata attgggctta taatattgca gatgattata aagctttaat tatttacgat
    10741 acgatgtgga aatccactca aaaacttgct tatactattt ctgccgcatt tgaagcagaa
    10801 gggattagag ctcaattagt taatttgcag accaatcata tttcagatat tatgactgcc
    10861 gtgatggatg ccaaatttat tgctgtcggc tcaccaactt taaacagttc cattttgcct
    10921 acggtagctg cctttcttta ttatttaaaa ggactttcac cccggaataa gattggtctt
    10981 gctttcggaa gttacggttg gggtggacaa agtataccaa ttttacatca gcttttagga
    11041 gaccctaaag aatgcggttt tgcaatgctg gaaccggttc aagtgcaata tattcccgat
    11101 gaagaagaac ttaaccggat aaaaagcaat ttgcaacggc aaataaggga aaaactaaat
    11161 gcaatgtaat aacttcaggg cagattttcc tgcctgcaac tggtcaaaaa aacataacta
    11221 aattaaagga agctgatatg aaatctttac cgcaaaaaga cctgtatgcg aaaattttta
    11281 atagcgcctt agttgctatc ggaacaaccg atacaaaagg aaattttatt gccgtaaatc
    11341 ctgcctggtg taaagcgctg ggctggacgg aagaagaaac taataccctt aatcttaaag
    11401 atataactct tgctgaagaa caacagaccc taaatcagga attgagtaaa ttagctgagg
    11461 gaaaggtttg cagtttgcgt aaaacacaac gctataaacg aaaggatggc agtttttttt
    11521 gggctgatct ttatcgttcc gcattgtttg atgataaagg caaagttatg ggcattctgg
    11581 atatttttgt ggatattgat gttcaggtta ggtccgaaaa agttcaggga gaattgtttc
    11641 agagtttgga aactttgaat aaggaactga gtaacgccaa tataggcctc aaacgacagg
    11701 caaaatatga ttctctaacc ggactttata acagatgggt aatggaagaa atgctgaata
    11761 aagaaattat aagctctctg gcaactaacc gcggttttgc tgttggtatt gctgatattg
    11821 atgattttaa aaaggtaaat gatacttatg gtcacgattg cggagacctt gttctggtaa
    11881 agctaactca atcctttttg cagaaaatca gagtaacaga ttcagtatgc cgttggggtg
    11941 gagaagaatt tttgttcctt ttttccgaaa cgacggaatc aggagcaatg atagttgtag
    12001 aacgcattcg gaaagctatt gaagaactta ctattaacta taaaggcaat cagattaagg
    12061 ttacaattac tattggggtt agtttttata acgggcaaga ccatctggat agcaaagaac
    12121 ttgtagacag agcagataaa gcaatgtatg aaggtaaaag cagcggaaaa aataaagcgg
    12181 tgttatattc aaatattccc tgatttttac gctgtatacg gattttacaa tgccttgtaa
    12241 ataaatctcg gtgttttcgc tgttctcgtt gtaaaaaaat cactaaagaa agcagttgac
    12301 ataaaacaaa gttcttctat tgtgtctcta tgaaataatg ggagatagta aatgaagaca
    12361 aattcacgca gctggcaacc tactcttact ttagccagat tatttgaaga acagaatcaa
    12421 ttttatgatg cattagctgc ttatgagcta atcagccaaa atgatccttc tccggaatta
    12481 cgggaaagga tagaggacct tcatctgcgt attttaaacg atcctaatag aacttacgat
    12541 ccccgtttgg agcaactctt ttctccggaa gaacttgcct atttaaaaat ccttgaccat
    12601 cagggtttcc tcaatatggc taaagcacgg gaaaagctaa tggaagaaat gcagggttca
    12661 gaattgattt tcagtgaagt tatgcctgaa gatgttttag ttgatgaatc cgaacttgat
    12721 accttaagag aaattctgga agaaattgaa cagcaggcac agctaagttt aatttctgaa
    12781 acaggtgatg ttggcgaata caatgttcaa gatttcttaa ttgcgatgct ggctaaattt
    12841 gataaagacc aaaaactgag cgaggttaaa ttgagtgacc tattatcgct atttctggag
    12901 atgcagagct taaaaaatca gccctgatga agcaaaaaca taccttgaac atcaattccc
    12961 tttgcgcaag ctgttatgaa aaatgtaaac aaaaggattt ggtgcttatt attacctgtc
    13021 ctttttatga tccttttccc gtgcagctgg aattgaaatt taagggattg cgtaaaaaac
    13081 ccaagaataa gtgataattc ctgctggcat aggaatgaca ataaaaaaca cagtaaagaa
    13141 gaaggaattt tcattctaat aaaaggatat gagtttcaaa tgcgtattat aaccggtatc
    13201 tataaaaaaa gaaacctgtt tttagttcct ggaatgagta ccagacctac ctcttctttc
    13261 aatcgggaag ttattttcag cgttcttcag gattatgcag gtttcagggt tttagacctt
    13321 tttgccggaa gcggttcttt aggtttggaa acactttcac gaggagctgt gtgggttgat
    13381 tttgtagaat ttgctccttc cgctatcagt actattttgc agaatataaa tcttttaggt
    13441 tgttccgaga attgtcatct ctggcgaaaa aaggtggatg tatatttgaa atcctgtgaa
    13501 aataaatacg atatcatatt tattgatcca ccttataaca aaaatttgat taaccccact
    13561 ctgaaactta tctatgaaaa gtcactactg aatgaggaag gtagcattat tgtggaacat
    13621 tcccctcagg aaatcatcgc agaggaattt ttgccctata ttacaaagga aaagtatttt
    13681 aaaaggtctc attttacctg gctgagggca aggaataatg atttaaatca ggagtcgtca
    13741 ctgccgaaat aacttttaaa ctattcacga atttgtttgg catttaggac taatgacgac
    13801 tggtttcaag cagattaaaa atatgaattt ttagccaccg aagccaccga a
//
LOCUS       DAOOZV010000049        13511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4559_length_13511_cov_64.409408, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000049 DAOOZV010000000
VERSION     DAOOZV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13511
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_13511_cov_64.409408"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1301)
                     /locus_tag="PLW08_05860"
     CDS             complement(24..1301)
                     /locus_tag="PLW08_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPL52162.1"
                     /translation="MKLLKSVSLPTSSTALKTVNVLFEDKIIKLSTDEIEVEEDVQII
                     DAKGLLMLPGGIDPHTHILAKNADAATDLAKITKIALEGGWTTLAELSYHTQSPVFNN
                     SALIKKIALVNANSFTSLALWGHIDIGDYPYHAESAQEIWSKGIVGIALAVPSPNPAI
                     PDITFTEIMDLFFDIYESDTSFAFQGYDYESYQEFSPLAQLDAIKKILRRMQENPIHI
                     PRISSYPTIEFINSVSKRSDISFATCISDLMALYNPDVFSTPFLCDYADFADLLFDLL
                     RSNKIYLLSNCVSPPVKNPNYCELFQDCNPELMNYSYLWVLSELWKKRKVPLATCLKM
                     TSENAAKRLGIYPEKGSLDPGSDADFVLYDPEATTIVKGNKGKNIELSGSFKAIYLKG
                     NQVAGKDFATIQQGSFLARSTNPKRRHNNSSWI"
     gene            complement(1391..2245)
                     /locus_tag="PLW08_05865"
     CDS             complement(1391..2245)
                     /locus_tag="PLW08_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52163.1"
                     /translation="MCRKWLFLILLILTACSTVKIPVEPQAKIDADNHLRFALQMENN
                     RDFSSARESYIFLTENYRSFADIKGMLACLSGLARIELENNNEKAYLQYKNQMAEMIQ
                     NTAPELAYYLLLLELYNYQLENNYSAISQKAISKIDYPAEVNLKLAIDKLQADSYLAI
                     AEEKQVKELINLSKKMEKQQKKAKYNDPELLSSAWYALAYYYYRTADYETANFYADKA
                     QDWDYRFGNFRGMAHCLWLKAQIAAAENNIPTAKRYWKKAEGIFSSLRDNVSLEELNT
                     TMQKLLKE"
     gene            complement(2235..3317)
                     /locus_tag="PLW08_05870"
     CDS             complement(2235..3317)
                     /locus_tag="PLW08_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPL52164.1"
                     /translation="MIDKIKHSRKIVFAFISIPVLLLLLAMVFIAIRQNLLEKKYVYY
                     STLANAMGISTQTPVLYKGFEVGRVRDFSLMEDGNIGLEFYILNRYKNIMVTGSILAR
                     NTNPITGKTTLEFIQNPNSREHLQDKSTILSSDFPEGKKQLKLIAPQASDPISLILDN
                     LAQLSSSLNEDYNADKGSIPRFLVNLADASEKANASMNKVEEITTELSILTANLNQDN
                     NPESGVLLRTLNNLAELTQGLNNRMNELEFILASAQTAIDNYKKPDSLLIKLLDPEQD
                     KILQPLSETLNNLESATAELVKILATVNNPELRLLLSNLNDSLAKARKTLEGLNNNPL
                     LRKGISPSAEQNMPSPKYIHQVPNVP"
     assembly_gap    3359..3458
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3459..3782
                     /locus_tag="PLW08_05875"
     CDS             <3459..3782
                     /locus_tag="PLW08_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52165.1"
                     /translation="LFCVAGGHRSSVILNIIKKAELGKAPTTLLGVIYALITMIREIT
                     SLKYMTNDDSKKAICIKRILWIHPEFNLKYLFLKTLLLHILFLYYQITNISSWLKSPL
                     SFLRK"
     gene            4054..5193
                     /locus_tag="PLW08_05880"
     CDS             4054..5193
                     /locus_tag="PLW08_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPL52166.1"
                     /translation="MKFKDSSQLTIPIMEEMPKFNSVVILNQEKLIEVLEEIEKIDNV
                     ILVKNRILALKAIINNYNNDREGLLNIDSNGETISLRKDILFSELNQILESQTLERAQ
                     YYLKRLKNGIKEEKTSKINDINLHRWKEYDDILTDSLWLFDKRNTSGAHLGWYWGNFI
                     PQIPHQMMLRYTKKDDWVLDTFLGSGTTLIESKRLGRNGIGIELNAEVANKAEKLIQM
                     EANKYDVISNVIIGDSRTIDLKPTLQKYNINKFQLLIMHPPYHDIIRFSKDDKNDLSN
                     TETTEEFLEMFKAVVNNVTSYLEKGRYLVLVIGDKYSKGEWIPLGFYCMQEVLNSGYL
                     LKSIIVKNFEETRAKRNQQELWRYRALVGGFYVFKHEYIMLFKKK"
     gene            5207..5524
                     /locus_tag="PLW08_05885"
     CDS             5207..5524
                     /locus_tag="PLW08_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52167.1"
                     /translation="MPLYSKKEILNLKLNSIKSKELRVLAENLGISTKGSAAEIIKRI
                     LEMKPQPPENIVDDYIKSIFQPSIQERKELISDDDLKNELSKVKSFSWGTKQGRNFFS
                     SRG"
     gene            complement(5565..7859)
                     /locus_tag="PLW08_05890"
     CDS             complement(5565..7859)
                     /locus_tag="PLW08_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPL52168.1"
                     /translation="MKIAMKIILCIMLFLPALISATLPTCYYNYAQIYALLQSHQQSH
                     PDIAKIYTIGYSQQDHIPIYAIRISDNVEIDEYEPALLFVGQVHAEEVLGVQTTMANI
                     AEILTYRYQQPYAQWISQLDLWFIPTLNPEGHNVVTENLDVSYRKNKRDNNNNGIFDF
                     DPVTGGDIDGVDINRNFAFNWVHGDTLYCPVGDETYDYYRGPAPMSESEIIGFQQLCE
                     QRKFIYAVCWHSSRTGNFSEKVYYPFNWKYIRPSPDLNFAASIATGFASQIIKQSGTG
                     TYEAYPNLSRQGAFHDWLYQQYGTIALLVECGTSDIQPDSTVMVGTVQRCSNGIRWLI
                     NRALMFSSAVPSSSMLTGTVTAAGIPTEAEIIIQQHNAPWFRPRTSFPQNGKFYRPLA
                     SGSYTVIARKKGYYDKIINNQIVNNGSWTNIQINLEPKPPATLRIGVRCSSEPVSARV
                     IIGDIFPDTLFVNGFANLNTYAGDYPIQIYALGYTPYIGTVNLTEGHNHLEFNLSPAT
                     IVFSEDWENGSSNWEIEGPWKVQNQIAASGYAITDSIGGYGFYAINCNVWIKTINPIL
                     IPATNQTWLIFDSHLYTEWNYDPCRVEISPDGNNWQEIWLKSGQWDSFRKEFVSLSDF
                     AGQSIYLRFRLTDSSNDIDLTDPGWTLDNIMIVCGTATENSDLNNDLTLLTLLYKNYP
                     NPFNPETTLSFTLAKNSRATLKIYNQKGQLVKTIVDTDLAKGDYRYVWNGKDNSGHPV
                     ASGVYLYSLVADGKTQTHKMLLLK"
     assembly_gap    8024..8123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8229..8438
                     /gene="rpmE"
                     /locus_tag="PLW08_05895"
     CDS             8229..8438
                     /gene="rpmE"
                     /locus_tag="PLW08_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPL52169.1"
                     /translation="MKKGIHPKYELVTVTCACGNTFQTRSTKDKLQVEICNVCHPFYT
                     GKQKIVDTAGRVEKFNKKYNLKQEN"
     gene            8518..9492
                     /locus_tag="PLW08_05900"
     CDS             8518..9492
                     /locus_tag="PLW08_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPL52170.1"
                     /translation="MQEKKEINVGGQAVIEGVMMRGPDKLATAIRRKDGSIELLKTPF
                     ISITQKKKFLGLPIIRGFVSLIEMMIIGIKTLTFSANRFELDLLAEEESKGKKTKKRS
                     KTAENTTNIVSYIIAFGLAFLLFGWLPYKLADWMHLSRKDILFNLFAGVIRIVFFVLY
                     VYLISLMKDIKRLFRYHGAEHKNVNAYEHNCPLIIEKIQDWSTIHPRCGTSFIFFVLL
                     ISILIFSLVDTLVSAYILHTPVPVYLRLAYHILLLPLVSGVSYEVLKYSGKNLNHPIV
                     KLLTVPGMALQHITTQPPDNDMIEIGLVAMKAALDMDLSEHKVKIIEE"
     gene            9498..10565
                     /gene="prfA"
                     /locus_tag="PLW08_05905"
     CDS             9498..10565
                     /gene="prfA"
                     /locus_tag="PLW08_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPL52171.1"
                     /translation="MLLQEKLQILENELKEMQEKISDPAVINDAQKYRDCLRRFKELR
                     EINAAWKHYQTLKKHIEEVNALEKTENDPEMLDLIKDEERSLQQQLEQTELKLKDLLI
                     PADPNDAKNAIVEIRAGTGGEEAALFAADLFRMYSYYAEMKGWKLEVISMNQTELGGY
                     KEIIFQLSGKDVYSYMRFESGVHRVQRIPVTESSGRIHTSAVTVAVLPEAEDIDIDIQ
                     ESDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGIVVTCQDEKSQIKNKAKALKVLRS
                     RLLDEEISRQEQEIAKARKAQVSTGDRSAKIRTYNFPQSRVTDHRINLTSYNLDSFLA
                     GNIEEFIQALRLAWRNEKVNT"
     gene            10566..11831
                     /locus_tag="PLW08_05910"
     CDS             10566..11831
                     /locus_tag="PLW08_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPL52172.1"
                     /translation="MSVVQIVLILLVLLFFLLLCFLFAGLETGMLSIDQIKLEQEAKK
                     SKSKAAILQFVRQPDRFLGTTLIGNNISNVIMASLSTYLVNRLFAPVFDARYTSLVVG
                     IVVLIFGEIAPKAIFRDNAETLVPLLFPFLQFFYYLLKPFVMVVTWLNNFLRKLLKLE
                     EGYQYDYLTKDDLSFLLSQAANAETEDNQMEMIEDALDFTELDAGNVMVPRTEIVAIQ
                     ENTPLSEVINIVRKEGFTRYPVYRNNIDDIIGILIIFDILKKDCTPDMIAGDIIHEPL
                     FVPENMDLDVLLKEMQTKQRSMAIVVDSFGGTAGIVTMEDILEEIVGDIEDEYDIDED
                     EHSVQQVGPNTWLVAADTEIDRLADDYGIELPEGDYETIAGLILDRLERIPHQGQVIN
                     IPPYKIQILQATAKQIIKVKIHKTSKIEV"
     gene            11833..13509
                     /gene="ftcD"
                     /locus_tag="PLW08_05915"
     CDS             11833..13509
                     /gene="ftcD"
                     /locus_tag="PLW08_05915"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPL52173.1"
                     /translation="MKLMECVPNFSEGRDKTILDAIATAIKNVKGVALLDIDPGADTN
                     RTVFTMAGEPEAVVEAAFQAIKKAAELIDMSKHKGAHPRMGATDVCPFIPISEMTMDE
                     CVEYAKQLGKRVGEELGIPVYLYEYAATKEEWRNLSNIRNGEYEALPEKAKDPYWKPD
                     FGPHTFNAKSGATAIGAREFLIAYNINLNTRDKKKASEIAQIIRESGRPARDENGKLL
                     KDENGNTIYIPGLFSHCKAVGWFIESYDRAQISINLTNYKITPPHLVLEKVRELASEM
                     GMQVTGSELVGLIPKSALVEAGKFYLHRLQESTGIPEKMIMQTAIQSMGLAELAPFDL
                     NKKVIEYAIAGKDLLVNLKVDAFCDLLSTDAPAPGGGSVSALCSAISGALSAMVANLT
                     IGKKGYEKVTEEAMKLAPIGQEIKQKSLEVIDKDTNAFFALMDAMRLPKKTEEEIAYR
                     KAQIENCTQQAINAPLETMRLAFSALELASKIAKIGNTNALSDAGVAALTAFCASQSA
                     YYNILINIPGITDEEFKQKTLAEAKELLEKCKNIADSVEKYVCEQLKSKG"
BASE COUNT         4330 a         2281 c         2642 g         4058 t
ORIGIN      
        1 agaaaggttg ttttaactca aggctaaatc caggaagaat tattatgcct gcgtttggga
       61 ttagtgctgc gggctaaaaa agaaccctgc tgaattgttg caaaatcctt tccggcaact
      121 tgatttcctt ttaagtaaat tgctttaaag gagccggaga gttcaatatt tttgccttta
      181 ttacctttta ctatggttgt agcttcaggg tcgtagagga caaaatcggc atccgaacca
      241 gggtctaagg agcccttttc cggatagata cctaaacgct tagcggcatt ttcactggtc
      301 attttaagac aggtggcaag gggaactttt ctttttttcc agagttcgga aagcacccaa
      361 aggtatgaat aattcattag ttccggattg caatcctgga agagttcgca gtaattggga
      421 ttttttactg gaggagaaac gcaattggaa agcaggtaaa ttttattgga gcgcagaagg
      481 tcaaacagta aatcggcaaa atcagcatag tcacacagga aaggagtgct gaagacatca
      541 ggattgtaaa gagccatcag atcagaaata caagtggcaa aagaaatatc gcttcttttg
      601 gaaacgctgt taataaattc aattgtggga taggaagata ttctgggaat gtgaatagga
      661 ttttcctgca ttctgcgtaa gattttttta attgcatcca gctgagcaag aggactaaat
      721 tcctgataac tttcatagtc ataaccttga aaggcaaagg aggtatcact ttcatagata
      781 tcaaaaaaca aatccattat ttccgtgaaa gttatatcag gaatggcagg attgggagaa
      841 ggaacagcta aagcaatgcc tacaatgcct ttactccaaa tttcctgagc ggattctgcg
      901 tgataaggat aatcgccaat atcaatatga ccccaaagag ccagagaagt aaaagaattg
      961 gcatttacca aagcaatctt ctttatgaga gccgaattat tgaaaacagg gctttgagta
     1021 tgatagctaa gttcagcgag ggtagtccaa ccgccttcta aagcaatttt agttatttta
     1081 gccaagtcag ttgccgcatc agcgttttta gctaaaatat gggtatgggg atcaattcca
     1141 ccagggagca ttagtaaacc cttggcgtca attatttgga catcttcttc cacttcaatc
     1201 tcatcagtgg agagcttgat aattttatct tcaaacagca cattcactgt tttcagggct
     1261 gttgaagaag tgggtaggga aacggattta agtaatttca taatttattt ctatttgaac
     1321 caaagctgat ttccatcttt gttggaagca gtttttggtt tcccgtatat tattattcgt
     1381 ttcatttatt ttattctttc agcagttttt gcatagtggt gtttagttct tccagagaga
     1441 cattatcccg caaagagctg aaaattcctt ctgccttttt ccaatatctt tttgctgtag
     1501 gtatgttatt ttccgcagca gcaatttgag ctttcaacca caaacaatga gccatacccc
     1561 gaaaattgcc aaagcgataa tcccaatcct gagctttatc ggcatagaaa ttggctgttt
     1621 cataatcagc agttctataa taataatagg caagagcata ccaggcagaa gaaagcagtt
     1681 cggggtcatt atattttgct tttttctgct gtttttccat cttttttgat aaatttatca
     1741 gttccttaac ctgtttttct tcagcaattg caagataact atctgcctgc agtttgtcta
     1801 tagcaagttt tagattaact tcagcaggat aatctatttt gcttattgct ttttgcgata
     1861 tggcagagta gttattttcc agctgatagt tataaagttc cagaagcaga agataataag
     1921 ccagttcagg agctgtattt tgaatcattt cagccatttg gtttttgtat tgcagataag
     1981 ctttttcatt attattttcc aattctattc ttgccaaacc gcttaagcag gcaagcatac
     2041 ctttgatatc ggcaaaagaa cgatagtttt ccgttaagaa aatgtaactt tcccgggcag
     2101 aggaaaaatc cctgttgttt tccatttgca aagcaaaacg caaatgatta tcagcatcta
     2161 tttttgcctg tggttcaacg ggaattttta cagtagaaca ggcagttaga attagcaaaa
     2221 ttaggaataa ccatttacgg cacattggga acctgatgta tatattttgg tgaaggcata
     2281 ttttgttcag cagaaggact aatccctttc cgcaaaagag gattgttatt aagtccttcc
     2341 aaggtcttac gggctttagc caaactgtca ttcaaattac ttaacagtaa acgcaattca
     2401 ggattattta ctgtagctag aattttcacc agttcagcag tagcggattc cagattattt
     2461 aaggtttccg aaaggggttg taaaatttta tcctgctcgg gatcaagcaa ttttatcaaa
     2521 agagaatcgg gttttttata attatcaata gcagtttgag cactcgctaa aataaattcc
     2581 agttcattca tgcggttatt caatccttga gtgagttctg ccagattatt caaagttcta
     2641 agcaaaacac cactttcggg attgttatcc tgatttaggt tggcggttaa aattgaaagt
     2701 tctgtagtta tttcttcaac tttattcata ctggcatttg ctttttccga agcatctgcc
     2761 aaatttacca gaaaacgggg aatggaacct ttatcggcat tgtaatcctc attgagagaa
     2821 gaagaaagtt gagctaaatt atccaaaata agggaaatcg gatcactggc ttgaggagct
     2881 atcaatttta actgtttttt accctccgga aaatcggaag aaagtatagt acttttatcc
     2941 tgtaagtgct ccctggaatt gggattttga ataaattcca aagtggtctt tcctgttatc
     3001 ggatttgtat ttcgtgctaa aatggaaccg gtaaccatta tatttttata gcgattaaga
     3061 atataaaact ccagcccgat attgccatct tccataagcg aaaaatcacg cactcttcct
     3121 acctcaaagc ccttgtataa tactggagtt tgagtagaaa ttcccattgc atttgccaaa
     3181 gtgctgtaat atacatattt cttttccaga aggttttgcc tgatagcaat aaaaaccatt
     3241 gccagcaaca ataataaaac tggtatgctg ataaaggcaa aaacaatctt tcggctgtgt
     3301 ttgatcttat ctatcatttt ttaaccctat tatggcttgt tattataatt ttaaagtann
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tttttgtgta gctggaggac
     3481 accgttcctc cgtaatttta aatattataa aaaaagcgga gcttgggaaa gctccaacta
     3541 cacttttagg agtcatttac gcattaataa ctatgatccg ggaaataact tcattaaagt
     3601 atatgactaa tgacgacagc aaaaaagcaa tatgtattaa acggatttta tggattcatc
     3661 cggagttcaa tttaaaatat ctgtttctaa agaccttatt attgcatatt ttgtttttat
     3721 attaccaaat tactaatatc agttcttggc tgaaaagtcc tttgtcattc ctgagaaagt
     3781 aggaatccac aacagataaa aaatatttat gtatttccaa cagagattta tattattact
     3841 taatcaacaa atagatcaag ttaaaacctg tttccaagtg ataataatac ttcattttat
     3901 tcggtgttct ctgtgttctg attgtcttcg ggaaaaaaat aatactaacc ccggctatca
     3961 gttcttcatt attaaaatca caaaaaagaa cttgactgtt aaacagtata ttcagtaaaa
     4021 agaattaaac aagataagac aggattgata gagatgaaat tcaaggattc aagtcaattg
     4081 acaataccta ttatggaaga aatgccgaaa tttaatagtg tagtaatatt aaatcaggaa
     4141 aagttgatag aggttttgga agagattgaa aaaatagata atgtgatctt ggtgaaaaac
     4201 agaattctcg cgttaaaagc tattatcaat aattataata atgatagaga aggattatta
     4261 aatatagatt ccaacggaga gacaatttct ctacgcaagg atattttgtt ttcagaatta
     4321 aatcaaatct tagaatccca aaccctggaa agagcacagt attaccttaa acgtctaaaa
     4381 aatgggataa aagaagaaaa aacaagtaag ataaatgata ttaatcttca cagatggaaa
     4441 gagtatgacg atattttaac cgacagttta tggttatttg ataaaagaaa cacctctggt
     4501 gctcatttag gatggtattg gggtaatttt attcctcaaa ttccacatca gatgatgtta
     4561 agatatacaa agaaagacga ctgggtgttg gatactttct tgggaagcgg aacaacatta
     4621 attgaaagta agagattggg aagaaatgga ataggaatag aattaaatgc tgaagtagca
     4681 aataaagccg aaaaactaat ccaaatggaa gctaataaat atgatgtgat aagcaatgtt
     4741 attattgggg atagcagaac tatagacctg aaaccaactt tacaaaaata caatatcaat
     4801 aaatttcaac tgcttattat gcatccaccc tatcatgata ttatccgttt ttctaaagac
     4861 gataaaaatg atctttctaa tacggaaacc acagaggaat ttttagaaat gtttaaggca
     4921 gttgtaaata atgtaacatc ttatctggaa aaagggcgct atttagtttt ggttattgga
     4981 gataaatatt ccaaaggtga atggattcct ttaggttttt attgtatgca ggaggtctta
     5041 aatagtggtt atctgttaaa aagtatcatt gtgaaaaatt ttgaggaaac cagagctaaa
     5101 agaaatcaac aagaactttg gcgttataga gcactggtcg gcggttttta tgtttttaag
     5161 catgaatata ttatgctttt taagaaaaaa tagataggag aagtatatgc ctttatatag
     5221 taaaaaagag atattgaatt tgaaacttaa tagtattaaa agtaaggagt taagagtatt
     5281 agccgagaat cttggtattt ccacgaaagg ttcagcagcg gaaatcatta aaaggatttt
     5341 ggaaatgaaa ccacaacccc ctgaaaatat tgttgatgat tacattaaat ccattttcca
     5401 gccaagcata caagaacgca aagaacttat atctgatgat gatttaaaaa atgaactttc
     5461 taaagtaaaa tctttttcct ggggcaccaa acagggaaga aattttttct ccagccgtgg
     5521 ttaaatgcta ataacaaagg tgtaaggcga taaatgagaa aggattactt taaaaggagc
     5581 attttatgag tttgggtttt gccgtctgca actaaagaat agagataaac tccgctggct
     5641 acaggatgcc cactattatc ttttccgttc cagacatagc ggtaatctcc ttttgccaaa
     5701 tctgtatcca ctatggtttt taccagctgt cctttttgat tgtagatttt taaggttgct
     5761 ctgctgtttt ttgccagagt gaaactgaga gttgtttccg gattaaaggg attggggtaa
     5821 tttttataca gcaaagtaag cagcgtgaga tcattattga ggtcggaatt ttctgtggca
     5881 gttccgcata caatcataat attatccagt gtccaacccg gatcggttaa gtcaatatca
     5941 ttggaagaat cggttaagcg gaaacgaaga taaattgatt gtccggcaaa atcacttaag
     6001 gaaacaaatt cctttctaaa gctatcccac tgtccagatt tgagccaaat ttcctgccag
     6061 ttgtttccat ccggggaaat ttccactctg caggggtcat aattccattc tgtataaagg
     6121 tgggaatcga aaataagcca ggtttgattc gtagcgggaa tcagaatcgg gttgatggtt
     6181 ttaatccaga cattgcaatt aatagcataa aaaccataac caccaatgct gtcggtaatt
     6241 gcatatcccg aagcggctat ctgattttgc actttccagg gtccttctat ttcccaattg
     6301 gaacttccat tttcccaatc ctcactgaaa acaattgtgg caggactgag gttaaactcc
     6361 aaatgattat gaccttctgt aagatttaca gttccgatat agggagtgta acctaaagca
     6421 taaatttgaa ttggatagtc cccagcataa gtattcaagt tagcaaaacc attcacaaaa
     6481 agtgtgtccg ggaaaatatc accgataata actctcgctg aaacgggttc tgaactgcaa
     6541 cgaacaccta ttcttaaagt agctggaggt tttggttcca aatttatctg gatatttgtc
     6601 cacgagccgt tatttactat ttgattattg ataatcttgt cgtaataccc ttttttgcga
     6661 gctataacgg tgtaagaacc actggctaaa ggacggtaaa attttccatt ttggggaaaa
     6721 gaagttctgg gacgaaacca aggcgcatta tgttgctgga taataatttc cgcttctgtt
     6781 ggtattcctg ctgcagtaac ggttccggta agcattgaag aagaaggaac tgctgaacta
     6841 aacattaaag ctctgtttat caaccagcgg attccattac tgcaacgctg aactgttccc
     6901 accataacag ttgaatccgg ttgaatatca ctggttccac attcaactaa aagagcgatt
     6961 gtgccatatt gctgataaag ccaatcgtga aatgcaccct gacgactcaa attgggatat
     7021 gcctcataag ttccagtgcc actttgtttg ataatttgag aagcaaaacc ggtagcaata
     7081 gaagcagcaa aattaaggtc gggactggga cgaatatatt tccagttaaa aggataatag
     7141 accttttccg agaaattgcc cgtccgggaa gaatgccaac aaacggcata aatgaatttt
     7201 ctttgctcac aaagctgttg aaagccaata atttcacttt cactcattgg tgcaggaccg
     7261 cgataataat cataggtctc atctccaacc ggacaataaa gtgtatcccc atgcacccaa
     7321 ttgaaggcaa aattgcgatt tatatccact ccgtctatat cgcctccggt taccggatca
     7381 aaatcaaaaa tgccgttgtt attgttatcc cgtttattct tacggtagga aacatccaga
     7441 ttttccgtaa ctacattgtg cccttcagga ttgagagtgg gaataaacca taagtcaagc
     7501 tgcgaaatcc attgagcata aggttgctga tagcggtaag ttaatatttc ggcaatatta
     7561 gccatagttg tttgaactcc caaaacctct tcagcatgaa cttgtccgac aaatagcaaa
     7621 gccggttcgt attcatcaat ttctacatta tcggaaatac gaatagcata aataggaata
     7681 tgatcttgct gcgaataacc aatggtatat attttagcaa tatcgggatg tgactgctga
     7741 tgactttgca ataaagcata aatttgggca tagttataat aacaagtagg cagggttgca
     7801 cttatcaatg ccggcaaaaa tagcataatg cataatatta tcttcatcgc tatcttcatt
     7861 gtaacttcct ttgtgtagcc gaagctcgct gtaattcctt tttttcttgc agaggaatgt
     7921 tgtcctccgg ctacacagtt ataaagcaat tttcagtcca aacaactgtt ctttgagtta
     7981 aaatcggttt tatttatact gccaatctct ttttattaaa caannnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnccgggtt tcacctttcc acttttttac ttttttgtat
     8161 tgacataatt cagcgagcta atttttaggt ttacttgtat tgatttataa ataaggagaa
     8221 ttagataaat gaagaaagga attcatccca aatatgaact ggtaacagtt acctgtgctt
     8281 gtggaaatac atttcaaacc cggagcacaa aagataaatt gcaggtagaa atttgcaatg
     8341 tatgccatcc cttttacaca ggaaaacaga agatagttga tactgccgga agagtagaga
     8401 aattcaacaa aaaatataac cttaaacaag agaattaggg ttttacaccc gatatacgcc
     8461 attttgtccg tttcctgaat acgggaaaat ggcgtttttt ctatctaatg gagaaaaatg
     8521 caggaaaaga aagaaatcaa tgttggcggt caagccgtta ttgaaggcgt gatgatgcgc
     8581 ggtccggata aattagcgac agctattcgc agaaaagatg gcagtattga attattaaaa
     8641 actcctttta tcagcatcac acagaagaag aaatttttgg gtctgcctat aattcgtggt
     8701 tttgtttcgc tgattgaaat gatgataatt ggcataaaga ccctcacctt ttctgccaat
     8761 cgttttgaac ttgatttact agcggaagag gagagcaaag gcaaaaaaac aaaaaaaaga
     8821 agtaaaacgg cagaaaatac aacaaacata gttagttata taattgcctt cggtttagct
     8881 tttctgcttt ttggctggct gccatataaa cttgcggatt ggatgcatct ttctcgcaaa
     8941 gatattctat ttaatctttt tgccggagtt attagaattg tcttttttgt gctctatgta
     9001 tatctgattt ctctaatgaa agatatcaag cggcttttcc gttatcatgg tgctgaacat
     9061 aaaaatgtga atgcttatga acataattgt cccttgataa ttgaaaaaat acaggactgg
     9121 tcaacaattc atcctcgctg tggcacaagt ttcatctttt ttgtgctctt gatcagtatt
     9181 cttatttttt ctcttgtaga tacattggtt tcagcgtaca ttttacatac tcctgttccg
     9241 gtttatttgc gcttggcata tcatattttg cttttacctc ttgtttcagg tgtatcttat
     9301 gaagtgctga aatattccgg gaagaattta aaccacccta ttgtaaaact tttaactgtt
     9361 cccggaatgg ctttacaaca tataactact cagccaccag ataatgatat gattgaaatt
     9421 ggtttggtag ctatgaaagc tgctttggat atggatttaa gtgaacataa ggtaaaaatt
     9481 atagaggaat aaatctcttg cttctccaag aaaaattaca aatcctggaa aatgaactga
     9541 aggaaatgca ggaaaaaatt agcgatcctg ctgttattaa cgatgcccaa aaatatcgcg
     9601 actgcctgcg tcgctttaag gaattaagag aaattaatgc tgcctggaaa cattatcaaa
     9661 ccctaaaaaa gcatattgaa gaagttaatg ccctggaaaa aacggaaaat gatccagaaa
     9721 tgcttgattt gataaaagat gaagagcgtt ctttgcaaca acaactggaa cagacagaac
     9781 tgaaactaaa ggatttgctt attcctgctg accctaatga cgctaaaaat gccatagtag
     9841 aaattcgtgc cggaacaggt ggcgaagaag cagctctttt tgctgccgat ttattcagaa
     9901 tgtatagtta ctatgcagaa atgaagggtt ggaaattgga agtgatttct atgaaccaaa
     9961 cggaactcgg tggctataaa gaaatcattt ttcagctaag cggtaaagat gtatattctt
    10021 atatgcgctt tgaaagtggt gtccatagag tgcaaagaat tcctgtaacc gaatcctcag
    10081 gcagaattca tacttctgca gttactgttg ctgttttgcc tgaagcggaa gatatagata
    10141 tagacattca ggaaagtgat ttacgcatag atgtttacag aagtacggga catggcggtc
    10201 aaagtgtaaa tacaaccgat tccgcagtta gaattaccca tttacctaca ggaattgttg
    10261 ttacctgcca ggacgaaaaa tcccaaatta aaaacaaagc taaagcactg aaggtgttac
    10321 gcagtcgctt attggatgag gaaatcagcc gccaggaaca ggaaattgcc aaagcaagaa
    10381 aggcacaggt ctctacaggt gatcgcagtg ccaaaatcag aacttacaat tttccccaat
    10441 caagagttac agatcataga ataaacttga caagttataa tctggattcc ttcttggcag
    10501 gaaacataga ggagtttatt caagcattac gacttgcctg gagaaacgaa aaggtgaaca
    10561 cataaatgtc tgtggttcag attgttctga tcctgttggt gttacttttc tttttattgc
    10621 tctgcttttt atttgccggt ttggaaacag gaatgctttc catagaccag ataaaattag
    10681 agcaggaagc gaaaaaaagt aaaagtaaag cagccattct gcagtttgtc cgtcaaccgg
    10741 atagattttt agggacaact ctaatcggca ataacatttc taatgtgatt atggcatcgc
    10801 tttccaccta tttagttaat cgtctttttg ctcccgtttt tgatgcgcgt tacacttctc
    10861 tagtggtggg catagttgtg ttgatttttg gtgaaattgc tccaaaagca atttttcggg
    10921 ataatgccga aacccttgtt cccctattat ttcccttttt gcaattcttc tattatttgc
    10981 ttaagccatt tgtaatggtt gttacctggt tgaataactt tctgcgtaaa ttgctgaaac
    11041 tggaagaggg ttatcaatat gactatttaa ctaaggatga cctttccttt ttgctttctc
    11101 aggcagctaa tgccgaaacg gaagataacc aaatggaaat gattgaagac gctttggatt
    11161 ttaccgaact ggatgcagga aatgttatgg ttccgcgcac ggaaattgta gctattcaag
    11221 aaaatactcc tctttcggaa gttatcaata ttgtaaggaa agaaggtttt acccgctatc
    11281 ctgtttatcg gaataatatt gatgacataa tagggatttt aattatattt gatatcttga
    11341 aaaaggattg cactccggat atgattgctg gagatattat tcacgaaccc ctttttgttc
    11401 ctgaaaatat ggatttggat gttcttttaa aagaaatgca aaccaagcaa cgcagtatgg
    11461 ctattgtagt tgattccttt ggtggaacgg caggaattgt aacgatggaa gatattttag
    11521 aagaaattgt aggtgatata gaagatgagt atgatataga tgaagatgaa cattctgtcc
    11581 agcaagttgg tcccaatacc tggctggtag ctgctgatac ggaaattgat agattagcgg
    11641 atgattatgg cattgaatta cctgaaggtg actatgaaac aatagcaggt ttgattttag
    11701 accgcctgga aagaattcct catcagggtc aggttattaa tattccaccc tataaaatac
    11761 aaattttgca ggcaactgca aagcagatta ttaaggttaa aatacataaa acgagtaaga
    11821 tagaggttta atatgaaact gatggaatgt gttcccaatt tcagtgaagg aagagacaag
    11881 acaattctgg atgccattgc aactgccatt aaaaatgtga aaggggttgc tttactggat
    11941 attgatcctg gagctgatac taatagaacc gtttttacaa tggctggaga acctgaagca
    12001 gttgtagaag cagcttttca ggcaattaaa aaagcagcgg aactaattga tatgagtaaa
    12061 cataaaggag ctcatcccag aatgggagct accgatgttt gtccctttat tccaatttcg
    12121 gaaatgacta tggatgaatg cgttgaatat gccaaacaat taggaaaaag agttggcgaa
    12181 gaacttggca tacctgttta cctatatgaa tatgcagcca ccaaagagga atggagaaac
    12241 ctgtctaata tccgtaacgg tgaatatgaa gcattacccg aaaaggcaaa agacccttat
    12301 tggaaacctg atttcggtcc tcataccttt aatgccaaaa gtggagctac tgctattgga
    12361 gccagagaat ttctcattgc ctacaatata aacctcaata cccgtgataa aaagaaggca
    12421 agtgaaatag cacaaataat tcgggaaagc ggaagacccg ccagagatga aaacggtaaa
    12481 ttgctgaaag atgaaaacgg taacactatt tatattccag gtttattttc tcattgtaaa
    12541 gcggtgggtt ggtttattga aagttatgac cgcgctcaaa taagcataaa cctgacaaat
    12601 tataaaatta cccctcctca tctggttttg gaaaaagtgc gggaactggc atctgaaatg
    12661 ggtatgcagg ttaccggaag cgaacttgta ggtttaattc ccaaatccgc tttagtggaa
    12721 gctgggaagt tttacttaca tcgcttacag gaaagcaccg gcattccgga aaaaatgatt
    12781 atgcaaacag ctattcaatc aatgggactg gcagaactgg ctccctttga tctaaataaa
    12841 aaggttattg aatatgccat tgccggaaaa gacctgctgg taaacctgaa agtagatgcc
    12901 ttttgtgatc tgctttctac agatgcacct gctcctggag gaggaagtgt ttctgctctt
    12961 tgttctgcta taagtggagc tctttctgca atggtagcta acctcacaat cggcaaaaag
    13021 ggctatgaaa aggtaactga agaagcaatg aaattagctc caataggaca ggaaatcaaa
    13081 caaaagtcac tggaagttat tgataaagat actaatgcct tttttgcttt gatggacgca
    13141 atgcgtttac cgaaaaagac agaagaggaa attgcatatc ggaaagcgca gattgaaaat
    13201 tgtactcaac aagcaattaa tgcacctctg gaaacaatgc ggttggcatt ttccgcttta
    13261 gagcttgcct ccaaaatagc aaaaataggc aataccaatg ctctttccga tgcaggagtt
    13321 gctgctttaa cagctttctg tgcatctcaa tctgcctact ataatatttt aattaatatc
    13381 cccggaatta cggatgagga gtttaaacaa aaaacccttg ccgaagcaaa agaactgctg
    13441 gaaaaatgta agaatattgc cgatagtgta gaaaaatatg tttgtgaaca gttaaaaagt
    13501 aaagggtgaa c
//
LOCUS       DAOOZV010000050        13486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4575_length_13486_cov_69.439282, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000050 DAOOZV010000000
VERSION     DAOOZV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_13486_cov_69.439282"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1069)
                     /locus_tag="PLW08_05920"
     CDS             complement(185..1069)
                     /locus_tag="PLW08_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52174.1"
                     /translation="MKNILYLVALSIVLISLSSCKLFKSPTVTKIQDVKVISITPDKS
                     TIKISFMVSNPNCYKLKLDQLNVELLNKDRYKVGNAYLQNVVEIPKKSAMCLDFNVEL
                     ETRPVVKMVSSIDQKVQFFITGKGHGKVMGIGKKFKFDEPYELDLKEQLKSIIPKFST
                     NGQSLFKITKTTVEKIGIGETELQVHFIILNPYGLTFNFKGFPAEIYISDKFAGTGNL
                     KNQLKFNENIYSQDGVMVFKLNNLKTILGAVKGAVKGEIRYSVKGRVLIDALGMEINQ
                     PYSYQDALPVNILEMLLN"
     gene            complement(1069..3180)
                     /gene="asnB"
                     /locus_tag="PLW08_05925"
     CDS             complement(1069..3180)
                     /gene="asnB"
                     /locus_tag="PLW08_05925"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPL52175.1"
                     /translation="MCGIAGIYCFSKETVILDKKQDNFSKGKDKPVKNAVSFKQNVAN
                     ALLLKEMTDAIKHRGPDDEGYLIKDKNGIRSFSGVDSPAEIQSVYPLFNVEIDSTFPA
                     KGYSSVNSFKMGNFILGMGFRRLSILELKPIGHQPMYDKELGIAICYNGEIYNYLELK
                     AELKAKGYKFFSNSDTEVIIKAYHFWGQDCVLHFNGMWSFSLWDERKDLLFCSRDRYG
                     IKPFYYAIQDGVLYWGSEVKQLLLTPIDKKLNQAMIWRSMKINSFLVYDDETYWQNVH
                     SLKPGHNLLAVNGKIMINQYYDLDIANFESSQLSFHKAVEQYRNIFLDSISLQLRSDV
                     EIGATLSGGMDSSAIVCSAVQKKGEPLKTFSSYYAFTPELDERKWMEKIVQKTSCTSF
                     LVSPKAEDAICWWEKLTYMNDLPLSAAFVSINAVMQEAHKQGIKVLLSGQGSDEISAG
                     YRHSLYRYFADLIRGMQISRLGKELPIYLKKDNKAFSKLGKIMLSTFLPESSLYNLEF
                     CYYRFEPFNRAFTNEAEKNCGEQILKKIADIKASRLSNFLYNMMHNTSLQTLLHFEDR
                     LTMANSVESRVPFLDYRLVDFVFSLPSQYKVQPPYTKVIHRFAMKDLIPEEIYYRQDK
                     GIFSSPFYQVWMKQELKPFISDIFASSAFRQRGIWNLPKINHYWHKYLAGDNKQVEML
                     FNVIALELWFRQYVDKPSGEY"
     gene            4254..7490
                     /locus_tag="PLW08_05930"
     CDS             4254..7490
                     /locus_tag="PLW08_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52176.1"
                     /translation="MSDLTKYIQKYRQRINLRFALQAIIKALISFLLALHIYFLLWLN
                     MPSQSMALFYANIGIRTCLILVIIYFLLSGYRNFWHNLAVARFLDKQIDFKDDLFQNT
                     LELAEKEKDNPIVENLGTMAKKRIEENRYKIPALYPSWIVFAILFLILGLGTIWAYSY
                     EDFRLAFKQFYTNQGQEVIYKNFIELSPGNLTIGRNEAVEIKILNPDPRLKHRLFYRI
                     DKQWRELGLADNRYIFQRLDNSIEYYAENEVCKSPVFKIQVLDEPIVKKWFVQYNPPA
                     YSGLPTWTDTLSYGNIEGLKHSRVKLSLTTNIPVESAVMVFDDASRLPLQAIDKNNYI
                     TQLAIEKPCSWYLELTDALGRKSKPEEKHIRILEDNPPEINITFPGQDVNLNQNMLLP
                     LIIEADDDYGLKNCTLSIQIMTSEPQVVNVQSVIPGKMFVTDFLLDLKDANLFPGDVV
                     TYWATIYDNSPEQQKAESTRFKARFPSIEEIYREIEAREQEKKTELETALQKSKDLQK
                     EFENKRRELLKQDNPKWEDKKQLEKILQEQENLSQQVQNIADNYQELINKMQANETLS
                     PETLQKMQKIQELMQEINNEDLQKAMEKFSNTLQNIKPEDLKKAMENFKFSMEDFSKK
                     IEQTLALLESIKKEQAIQKALQISEEMEKMQKTLSERTMDEKQNYKDLASDQKQISDT
                     YDKLQQELDKIDKMLESPRDNQVINQLKDLKQDMQNSNLKQDLQNSQNALSQNQRSQS
                     LSAQNQALEKIRRFSLKLNQMKESMGGGNQQRTMQAIQTAIRELLIFSKQHEATASRY
                     RNDPYVIVQDLIAQSEGIQISLNNMFRETQVLMIIPPKFFIDVNETNSAYRNIFSYIN
                     ESQFYQIPNALNSVQKGINLMVYDLMQALQNASSGTGSGGGGMQSLMQMLEQMSQEQM
                     AMNILTEQLFLQMQAQGGKMDSAMQQQIQRLAEDQERLAENLKRALQNNPEAQKQGNV
                     LKQITEEMDAITRQLKNNQLNPDILERQERIISKMLDAQRSINKREFTEKRKAETGED
                     MLYKGNTKIDLEALRRSGLLEEGLRAYPKEYQQVIMRYLKELNEAINK"
     gene            7487..9214
                     /locus_tag="PLW08_05935"
     CDS             7487..9214
                     /locus_tag="PLW08_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL52177.1"
                     /translation="MKKAALLILILFTCASAIYAQYDEKQILTQQANQLLMQRQYVQA
                     EQLFLRILDKYPNDTNSINQLMNIYFTLSQTDKAEATLTKYQRTLPPQTYSELLIQLL
                     IMQAKVEEAWQETMRYLNIYGQDEYKYRRLASFFERKAFYDKVIELYTRARNQLGKPD
                     LFRLEMANTAMNYRQFEKAIREYMAYTENNPVYMFYTNNQLKTILQEDATLISVVSAI
                     ADSSNSSVLKEIYANALLNLQDYKTALNIYKQLEPQKMWRFAEEQTAAGNDSIAFEAY
                     SYLETGETDMVKKMDLRYRLAEIKYQQRDYPATEQIIMESLSLPFWKDRNLYYRSSVG
                     VKLRKLMAEIKLAKNEPVDSALVWLNEAKKFSRNNLESQEMDLEIARLYILNGDKTKA
                     QNLLKTINHNTLMEKRDYLNFLNTLLEGEAALADTLMNEFIIRYPESQYANDAIYLMM
                     FTLNMAEQDKNSFYSAIRLLQLNRQSGIDTLVVIFSHNQDEEVLLLAIEWAIAFGNDS
                     KAKELLQYDFQDEVAADYAALLRLLMVADKAEEQRLAREFLKNKPNSIFSPDFRQKIS
                     RWSASRPNL"
     gene            9233..10690
                     /gene="guaB"
                     /locus_tag="PLW08_05940"
     CDS             9233..10690
                     /gene="guaB"
                     /locus_tag="PLW08_05940"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPL52178.1"
                     /translation="MNLRKAYTFDDVLLVPQKSDVLPAEVDLSTKITAQISLRIPVIS
                     SAMDTVTESAMAIAMAREGGLGIIHKNLSIEEQAKQVSLVKRAESGIVTHPYILSPED
                     TLDYVLALRDAHHIGGFPVVENEFLVGILTSRDIRFVTNPQTKVKDLMTPKEKLITAK
                     TGISLDKAIDLLQKHRLEKLLLISEEGKLEGMITVKDIMKRLNYPEAVQDNKNRLLVG
                     AAIGVTGDYLERAKELLNAGADLLVIDTAHGHHKNIGIALQKVKKHLNCQVIAGNVAT
                     ADACRYLIENGADAVKIGIGPGSICTTRVIAGIGVPQLSAIMDCALEAGKYNIPIIAD
                     GGIKFSGDIVKALAGGASAVMIGSLFAGCDESPGESIIYNGRRFKSYRGMGSLSAMKQ
                     GSKDRYFQDEQVEDNKLVAEGIEGMVPYKGPVKDFLYQLMGGLRSGMGYCGTATIQEL
                     QEKAEFIEITTAGLKESHPHDVHITKETPNYYSGD"
     gene            10644..11585
                     /locus_tag="PLW08_05945"
     CDS             10644..11585
                     /locus_tag="PLW08_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HPL52179.1"
                     /translation="MSTSLKKPRTITAGIEEELSPALKSYLSSFVYHLKVERGMAKNS
                     IESYRRDIGDFLLFCPKEIGNYETDDITKYLLSLQEIGLLNTSVARKRVALGQFFGFL
                     KDNDIETKVDMDLVPRIKLGVQLPDVLTVEEMLQLLNSLPVKTPLEIRNKLMMELLYA
                     TGMRISELLSISLHDLNLTERVILVHGKGSKQRYVPYVDTLDELFAKYLKQSRPILLK
                     LKQSELLFLNNRGEKMSRMGFWKILRKAVLEATIKKEVTPHTFRHSFATHLLEGGVNL
                     RIVQALLGHSSIDTTQIYTHIDMKRLVETYKEYHPRA"
     gene            11717..12724
                     /locus_tag="PLW08_05950"
     CDS             11717..12724
                     /locus_tag="PLW08_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL52180.1"
                     /translation="MKNLVNPFNPFCYIIIALTLVLCLSSCKTKSSKQEQAELQKVSV
                     TLDWTPNTNHTGIYVAKELGYFQEQGLEVDILQPGQNVTDQIVATGKSEFGVSYQENV
                     IRARSENIPLVSIAAVIQHNTSGFASLKKAGIKSPLDFEGKRYGSWDSPSELAILRAT
                     MEKYGADFNKVTVISGIYDFFSTIGKDADFEWIYYGWDGVEAERRGIALNYIPLKEID
                     PVFDYYTPVIISSEDYLAKNPETAKKFMTALKKGYEYCIAKPDSAAAILLKNVPELNA
                     EQVKRSMQYLAKEYQSDAESWGIQKLAVWKRFCEWMYQQRLIQLAVDYNKAFTNDYLP
                     Q"
     gene            12730..>13486
                     /locus_tag="PLW08_05955"
     CDS             12730..>13486
                     /locus_tag="PLW08_05955"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL52181.1"
                     /translation="MPNCILEAKDIRKTFLFKGALVVVLDKLQFSIQEGQFVSIVGPS
                     GCGKSTFLELLAGITKPDNGNIYLEGNEITGKTGFLGYMPQDDLLFPWLTTMQNILLP
                     VKVQGKDEKEARKKIIELLPLFGLMNYTDHLPYQLSGGLKQRVALLRTYMSSAEILLL
                     DEPLAKLDALTRSQLQTWLKDIVHLLNLTVILVTHDIDEAIMLSDRIDLMSINPGTFM
                     ESIYLDRNAPLNAEEQLKLKTKIRSKLVNGEEKS"
BASE COUNT         4513 a         2450 c         2611 g         3912 t
ORIGIN      
        1 agttccgtag gaacgacaga tagtatagag aggttttaac ctggaaaaaa aattcaaatt
       61 tatttttcgg tttactgaat tctgagttac cataaataga ggacatatat ttttcaggtt
      121 tctgcgaact ctttggcaaa gaggaagttg ccaacccatt tttcctcaat tgtattattt
      181 aagtttaatt cagcagcatc tctaaaatat ttaccggtaa ggcatcctgg taggaatagg
      241 gttggttaat ttccattccc aaagcatcaa taaggactct tcctttcacg gaatatctta
      301 tttctccttt aactgcgcct tttacagctc ccagaatggt tttcagatta ttcagcttaa
      361 aaaccattac tccatcctga gagtagatat tttcgttgaa tttaagttga tttttcaggt
      421 ttcccgttcc ggcaaattta tcgctgatat aaatttcggc gggaaagccc ttgaaattga
      481 aggttaatcc gtaaggattc aaaataataa aatgaacctg taattccgtt tcaccaatgc
      541 cgatcttttc aacagtggtc ttcgttattt tgaataaact ttgtccattg gtgctgaatt
      601 tgggaataat gcttttcagt tgttccttca aatcaagctc gtagggttca tcaaatttga
      661 actttttccc aatccccata actttgccat gtcctttacc ggttatgaaa aattgcactt
      721 tctggtctat ggaactaacc attttcacaa ctggacgcgt ttccagttcc acattaaaat
      781 ccaaacacat agctgacttt ttgggaattt ccactacatt ttgcaaataa gcgtttccca
      841 ctttgtagcg gtctttattt agcagttcca cattcaattg atccagtttc aatttatagc
      901 aattaggatt acttaccata aacgatattt taatagtgct tttatcggga gtgatggaaa
      961 tcactttcac atcctgaatt tttgtcactg tagggctctt gaagagtttg caggaactga
     1021 gagaaataag cactatgcta agagctacta aatataatat gtttttcatc aatattctcc
     1081 tgaaggttta tctacatact gtctgaacca aagttccaaa gcgataacat tgaatagcat
     1141 ttccacctgt ttgttatctc ctgccaaata tttatgccaa taatgattaa tcttgggcaa
     1201 attccaaatt cctctttgac gaaatgcact gctggcaaaa atatcggaaa taaaaggttt
     1261 taattcctgt ttcatccaga cctgataaaa gggcgaagaa aaaatgcctt tatcctgccg
     1321 gtaatatatt tcttccggaa tcaaatcttt cattgcaaaa cggtgaatta ctttggtata
     1381 aggtggctga accttatatt gtgacggcag agaaaaaaca aaatccacca aacgataatc
     1441 cagaaaagga actctacttt ccacagaatt tgccatagtc aaccggtctt caaaatgcaa
     1501 caatgtttgc agggaagtat tatgcatcat attatacagg aaattggaaa gccgtgatgc
     1561 cttaatatcc gcaatctttt tcagaatttg ttccccgcag tttttttcgg cttcgtttgt
     1621 aaaagcacga ttaaagggct caaagcggta atagcaaaac tccagattgt agagtgatga
     1681 ctccggcaaa aaagtggaaa gcattatttt tcccagtttg gaaaatgcct tattatcttt
     1741 tttcaaatag ataggcagtt ctttccctaa tctgctaatt tgcatacccc taatcaaatc
     1801 ggcaaaataa cggtataaag aatgtctgta accggcagaa atttcatcgg aaccctgtcc
     1861 cgaaagcaaa accttaattc cttgtttatg tgcttcttgc atcactgcat tgatagatac
     1921 aaaagctgca gataagggaa ggtcattcat ataagtcaat ttttcccacc agcaaatggc
     1981 atcttctgcc ttgggactaa ccaaaaacga agtgcaagag gtcttttgca cgattttttc
     2041 catccacttg cgttcatcca gttccggcgt aaaagcataa taggaagaaa aggtttttag
     2101 gggttcaccc tttttttgca ccgcagaaca aacaattgcc gaagaatcca ttcctccgga
     2161 caaggtagct ccaatttcta catcactgcg aagttgtaaa gaaatagaat ccagaaagat
     2221 atttctatat tgttcaactg ctttatgaaa agaaagctgg gaactttcaa aattggctat
     2281 atccaagtca taatattggt tgatcataat cttaccgttt acagcaagca aattatgccc
     2341 cggttttaaa gagtgaacat tttgccaata ggtctcatca tcatatacca gaaatgaatt
     2401 tatcttcata ctacgccaaa tcattgcctg gtttaatttc ttatctatag gggtaagcaa
     2461 aagctgttta acttcactgc cccaatataa tacaccatcc tgaatcgcat aataaaaggg
     2521 tttaatgcca tagcgatcac gactgcaaaa aagcaaatcc tttctttcat cccataaaga
     2581 aaatgaccac attccgttaa aatgaagcac acaatcctga ccccaaaaat gatacgcctt
     2641 aattattact tctgtatcgc tattgctgaa aaatttgtag ccctttgcct taagctctgc
     2701 ttttagttcc agataattat atatttcacc attataacat attgctatgc caagttcttt
     2761 atcatacatc ggttgatgac caatcggttt caattccaaa atgctcaagc gcctgaaacc
     2821 cattcccaaa ataaaattac ccatcttaaa agaattaacc gaagaatatc ccttagcagg
     2881 aaaagtgcta tctatttcaa cattgaataa aggatatact gattgtattt ctgctggtga
     2941 atctactcct gaaaaagacc taattccgtt tttatcctta attaaatagc cctcatcatc
     3001 aggaccccga tgcttgatag catccgtcat ttcttttaat agcagtgcat tagcaacatt
     3061 ttgcttaaaa ctgaccgcat tcttaaccgg tttatccttt cctttgctga aattatcctg
     3121 ttttttatct aaaataactg tttctttact aaaacagtag atacctgcta ttccgcacat
     3181 tcattctcct ttttctggta attccttttt tcataattac atttccagtc aagcgtttta
     3241 tcatttgata taaggaacaa atttattaaa ttatcaaagt tatgattgct ctgatggttt
     3301 accttttcaa tttcttagat tatatgattt tttccttttt atccatcaat cctgtaaatt
     3361 atgataacct tatttatata tttttctctc ttaatatcct tttatggaaa taaatatgta
     3421 taaactcaaa taatgccgta gaaataatgg ttaatattat tttgatattc attaatcata
     3481 tattgtagca aaaattccgt agaaatggca gttagataaa taagtgtgtt aaactgacga
     3541 aaaaatatcg ctaagaaagt gagataatta cataggaaag acagatattt tagaagggtt
     3601 ttaactctgg gaagataaga caaatttacc ttttgggttg ccaaattccg attcggcaaa
     3661 aaaaagaata aatgtttttg tagaattcta cgaactcttt ggcaaattgg aagttgccaa
     3721 cctattttca cgcaattgca ttatttagaa taaaatactc gttcacttgc tgaagtaata
     3781 atcttgtaac attcaagtaa cgaccaagta acggaactac tgcagtgtta gggaagtgtt
     3841 acggatgtgt tacggggtct tcttgcaaga gattatgttt caggtaatta agcgattagg
     3901 aatagaatag gcaaaatata taaataacca cttatgatat aagatgttaa aaagatatga
     3961 cagaaatttt gctggtggat aattagggag atatatgccg gaatttatag tcaattttta
     4021 tgtaggacat agaataataa atcgtgagta ggatgtaatg tttttaatta tttacgaggg
     4081 gcttacgcac ccatcgctat cattgtgtcg ccctgatggg ctttttttga gcgggaaaag
     4141 gaaaaaaaag aatgcggaag aacggcgtcc tccagctaaa tttgcaaaac ttcttgacac
     4201 aataagtgct ttataggaca aagatttttc aaagaagagg agaagacaaa aaaatgagcg
     4261 acctgacaaa atacatccaa aaatacaggc aaaggattaa tcttcgcttt gctttgcagg
     4321 caattatcaa agctctaatc agctttttgt tggctttgca catttatttt ttgttatggt
     4381 tgaatatgcc ttcgcaatct atggcacttt tttatgcgaa tattggcata cggacttgtc
     4441 tgattctggt gataatttac tttcttttaa gtggttatcg taacttttgg cataatttgg
     4501 cagtcgctcg ttttttggat aaacagattg attttaaaga cgatcttttt cagaatactt
     4561 tggaacttgc tgaaaaggaa aaagataatc cgattgtgga aaatttgggc acaatggcaa
     4621 aaaagcggat agaggaaaat cgttacaaaa ttcctgctct atatccttcc tggattgtct
     4681 tcgctatcct ctttctaatc ttgggtttag gaactatttg ggcatattct tatgaggatt
     4741 ttcgtttagc attcaagcaa ttttacacca atcaaggtca agaagttatc tataagaatt
     4801 ttatagagtt aagccctggc aacttgacta ttggcagaaa tgaagcggta gaaataaaga
     4861 tcctcaatcc cgatccgcgt ttgaagcatc gtctttttta caggatagat aaacaatgga
     4921 gggaattggg tttggcagat aaccgctata tttttcagcg acttgataat agtattgaat
     4981 attatgcgga aaatgaggtc tgcaagtccc cggtgtttaa aattcaggtt ttggacgaac
     5041 cgattgtaaa aaagtggttt gtgcaatata atccgcctgc ttattccggt ttgccaacct
     5101 ggacggatac tttaagttac ggcaatatag agggattaaa gcacagcaga gtaaaacttt
     5161 ccctcaccac taatattcct gtagaatcag ctgtaatggt ttttgacgat gccagtcgtc
     5221 ttcctttaca agcaatagat aaaaataatt atatcaccca acttgccatt gaaaaacctt
     5281 gcagttggta tttggaactt actgatgctt taggtcgcaa aagtaaaccc gaagagaaac
     5341 atattcgtat tctggaagat aatcctcctg aaatcaatat tacttttcca ggtcaggatg
     5401 ttaacttgaa tcaaaatatg ctacttcctt taatcattga agcagatgac gattacggat
     5461 tgaaaaattg cactttgagc atccaaatta tgacttcgga accgcaagtt gttaatgtgc
     5521 aaagtgttat tcccggaaaa atgtttgtaa cggactttct tctggatttg aaagatgcca
     5581 atttattccc tggtgatgta gttacttatt gggcaactat ttacgataat tctcccgaac
     5641 aacaaaaagc ggaaagcaca agatttaaag ctcgtttccc ttccattgaa gagatttata
     5701 gagaaattga agcccgcgaa caagaaaaga aaaccgaact ggaaaccgcc ttacaaaaat
     5761 ccaaagacct ccaaaaagaa tttgaaaata aacgccggga actgcttaaa caggataatc
     5821 ccaagtggga agataaaaaa cagctagaaa aaatattgca ggagcaggaa aatctttccc
     5881 aacaggtgca aaatattgcc gataactatc aggaactgat taataaaatg caggcaaatg
     5941 aaaccctctc tcccgaaacc ttgcaaaaaa tgcaaaaaat ccaggaactg atgcaggaaa
     6001 ttaataatga ggatttgcaa aaagcaatgg aaaaattctc taataccctg caaaacatta
     6061 aaccggaaga ccttaaaaaa gcaatggaaa atttcaaatt ctcaatggag gattttagca
     6121 aaaagattga acagacactc gccctgctgg aaagcattaa aaaagaacag gcaatccaaa
     6181 aagcattgca gatttccgaa gaaatggaaa aaatgcagaa aacacttagc gaacgcacaa
     6241 tggatgaaaa acaaaactat aaagaccttg cctccgacca aaaacaaatt agtgatacct
     6301 acgataaact gcaacaggaa ttggataaaa ttgataaaat gctggaatcc ccgcgcgata
     6361 atcaggttat aaatcaatta aaagacctga aacaagatat gcaaaacagc aatctgaaac
     6421 aagacctgca aaacagtcag aatgctttaa gccaaaatca acgcagtcaa tccctttctg
     6481 ctcaaaatca ggcactggaa aaaatacgcc gtttttcctt aaaactgaat cagatgaagg
     6541 aatcaatggg tgggggtaat caacaaagaa caatgcaggc aattcaaact gcaataagag
     6601 aacttctgat tttttccaag cagcatgagg caactgccag ccgttataga aatgatccct
     6661 atgtaattgt gcaagacctt attgctcaat ccgaaggaat tcaaatttcc ttaaataata
     6721 tgttccggga aacacaggtg ctgatgataa ttccccctaa attctttatt gatgtgaatg
     6781 aaaccaatag cgcctacaga aatattttca gctacatcaa tgaatcgcag ttttaccaaa
     6841 ttcccaatgc cttaaacagc gttcaaaagg gaataaacct gatggtatat gacctaatgc
     6901 aggcattgca aaatgcttct tccggaacag gaagcggggg tggaggtatg caatccctaa
     6961 tgcaaatgct ggaacaaatg agccaggaac aaatggcaat gaatatcctc accgaacaat
     7021 tgtttttaca aatgcaagct caaggcggaa aaatggatag tgctatgcaa caacaaatac
     7081 aacgcctggc agaagatcag gaacgcctcg cagaaaacct gaaaagagcc ctgcaaaata
     7141 atccggaagc acaaaaacag ggaaatgtcc tgaaacaaat tacagaagaa atggatgcca
     7201 taaccagaca gctgaaaaat aatcagctca atcccgatat cctggaaaga caggaaagaa
     7261 ttatcagcaa aatgctggat gctcaacgct ctatcaataa aagggaattt acggaaaaac
     7321 gcaaagctga aacgggtgaa gatatgctct ataaaggaaa tacgaaaatt gatttggaag
     7381 ctttaagacg cagcggactt ttagaagaag gtttgcgtgc ttatcctaaa gaatatcaac
     7441 aggttataat gcgatatctg aaagagctga atgaggcaat aaataaatga aaaaggcagc
     7501 tctcctaata ctgatactat ttacttgcgc aagtgcaata tatgcacagt atgatgaaaa
     7561 gcaaattctt acccagcagg caaatcaatt gctgatgcaa cgccaatatg ttcaggcgga
     7621 acagctcttt ttacgcatcc tggataaata ccctaacgac acaaattcca tcaatcagct
     7681 gatgaatata tattttactc tgtcccaaac agataaggca gaagcaacct taaccaaata
     7741 ccaaagaact ttgcctccgc aaacatatag cgaactgctt attcagctgc taattatgca
     7801 agcaaaagtt gaagaggcct ggcaggagac aatgcgctat ttaaacatct atggccagga
     7861 tgaatataaa tacagacggt tagcttcttt ctttgaacgc aaggcatttt acgacaaagt
     7921 gatagagctt tatacaagag caagaaatca attgggaaaa ccggatttat ttcgtttgga
     7981 aatggctaat acggcaatga attatcgtca atttgaaaaa gcaattcgtg agtatatggc
     8041 ttatacagaa aataatccgg tctatatgtt ttataccaat aatcagctga aaacaatatt
     8101 gcaagaggat gcaaccctta tttccgttgt ttctgctatt gccgacagca gtaatagttc
     8161 tgtgctgaaa gaaatttatg ccaatgcctt gctcaatttg caggattata aaacagctct
     8221 gaatatttat aaacagctgg aaccccaaaa aatgtggcgt tttgccgaag aacaaacagc
     8281 tgcaggtaac gatagcattg cctttgaggc atacagctac ctggaaacag gagaaacgga
     8341 tatggttaaa aagatggatt tgcgttaccg cctggctgaa ataaaatatc aacaaaggga
     8401 ctatccagct actgaacaaa taattatgga atcgctatct ctgcctttct ggaaggatag
     8461 aaatctctat tatcgcagtt ctgtgggagt taaattacgc aaattgatgg cagaaatcaa
     8521 attggctaaa aatgaacctg ttgatagcgc tttggtctgg ttgaatgaag ccaagaaatt
     8581 tagtcgtaac aacttggaat cacaggaaat ggatttggaa attgcccgcc tgtatatatt
     8641 gaatggagat aaaactaaag cccaaaacct cttgaaaacc atcaatcata ataccctgat
     8701 ggaaaaaagg gattacctga acttcttgaa tactctgctg gaaggtgaag ctgccttagc
     8761 ggatactttg atgaatgagt ttatcattcg ctaccccgaa agccaatatg ccaacgatgc
     8821 tatctacctg atgatgttta ctttgaatat ggcagagcag gacaaaaata gtttctattc
     8881 cgctattcgt ttattacaac tgaaccgtca aagcggaatt gatactttgg tagttatctt
     8941 cagtcataat caagatgagg aagttctttt actggcaatt gaatgggcta ttgctttcgg
     9001 caatgactcc aaagcaaaag agttactgca atatgatttt caggatgaag ttgctgcgga
     9061 ttatgccgct ttattacgac tgttaatggt tgcggataaa gcggaagaac aacgactggc
     9121 aagagaattt ttgaaaaaca aacccaacag tattttttca cctgatttta ggcaaaaaat
     9181 aagtcgttgg tcagcttctc gacctaattt atagttctta acgggagata aaatgaactt
     9241 acgcaaggca tacacctttg atgatgttct gctggtgcct caaaaatcgg atgttttacc
     9301 tgcagaagtg gatttgagca caaaaataac ggctcaaata tctttacgga ttcctgtaat
     9361 cagttccgct atggatactg taacggaatc cgctatggca attgctatgg caagagaagg
     9421 aggattggga attattcata aaaacctttc tattgaagaa caggcaaagc aggtcagttt
     9481 agtgaaaaga gcagaaagcg gaattgtgac tcatccctat attctttcac cggaagatac
     9541 tttggattat gttttggctt tgagagatgc acatcatatc ggtgggtttc cggttgtaga
     9601 aaatgaattc ctggttggta ttttaaccag tagagatatc cgttttgtaa ccaatcccca
     9661 aacaaaagtg aaagacctga tgactcctaa ggaaaaactg attactgcca aaaccggaat
     9721 ctcgctggat aaggcaattg accttttaca gaaacatcgc ttggaaaaac tcctactgat
     9781 tagtgaggaa ggcaaactgg aaggaatgat aaccgttaaa gatattatga aacgcttaaa
     9841 ttatcccgaa gcggttcaag ataataaaaa ccgtttactt gtaggtgctg caattggagt
     9901 tacgggtgat taccttgaaa gagcaaaaga acttcttaat gccggagccg atttactggt
     9961 aatagatacg gcacatggtc atcataaaaa cattggcatt gccttgcaaa aagtaaaaaa
    10021 gcatttgaac tgtcaagtta ttgcaggcaa tgtagctact gcagatgcct gcagatattt
    10081 aattgagaac ggagcagatg cagttaaaat tggcatcggt cccggttcca tttgcacaac
    10141 cagagttatt gccggaattg gagttccgca attaagcgcc ataatggatt gtgctttgga
    10201 agcaggtaaa tacaacattc caattatagc tgatggaggc ataaaattca gcggagatat
    10261 tgttaaagca cttgctggag gtgcttctgc agttatgatt ggttcacttt ttgccggttg
    10321 cgatgaaagc cccggagaat ccataattta caatggcaga aggtttaaaa gttaccgggg
    10381 aatgggttcc ttatccgcta tgaagcaagg cagtaaagac cgctattttc aagatgaaca
    10441 agtagaagac aacaaacttg tagccgaagg aattgaagga atggttccct ataaaggtcc
    10501 cgttaaggat tttctctatc aattgatggg tggattgcgt tccggaatgg gttattgtgg
    10561 aactgccact attcaggaat tacaagaaaa agccgaattt attgaaatca caactgccgg
    10621 cttgaaggaa agtcatcctc acgatgtcca catcactaaa gaaaccccga actattacag
    10681 cggggattga agaagagctt tctccggctt taaaaagtta cctttccagc tttgtttatc
    10741 atttgaaggt agagcgggga atggcaaaaa acagcattga gagctatcgt cgcgatattg
    10801 gtgatttttt gttgttttgc cctaaggaaa ttggcaatta tgaaacggac gatattacaa
    10861 agtatcttct ttctttgcag gaaattggac ttttaaatac cagcgttgcc agaaagagag
    10921 ttgccttggg tcaattcttc ggttttttaa aggataacga tattgaaact aaggtagata
    10981 tggatttggt tccgcgtatc aaactgggag tgcaattacc ggatgtttta actgtggagg
    11041 aaatgttgca gctgttaaat tccctgcctg ttaaaacacc tttggaaatt cgcaataagc
    11101 ttatgatgga attgctgtat gcaacaggta tgcgaatttc agaactctta agtatttctt
    11161 tgcacgattt gaatttaacg gagcgggtta ttttagttca cggcaaaggg agtaagcaaa
    11221 gatatgtgcc ttatgtagat acgcttgatg aactgtttgc aaaatactta aagcagagtc
    11281 gtccaatact attaaagctg aaacaatcgg aattgctttt tctgaataat cgcggggaaa
    11341 aaatgagtcg gatgggtttc tggaaaatat tacgcaaagc ggttttggag gcaactatca
    11401 aaaaggaagt aactccacat acatttcggc atagttttgc cactcattta ttggaaggag
    11461 gagtgaattt aagaattgtg caagcccttt tgggacattc ttccatagat acaacgcaga
    11521 tttatacaca tattgatatg aagcgactgg tggagacata taaagagtat catccacgcg
    11581 cgtaaaaaag gtgaacagcg aatggtgaac agtgaaaaaa aagaattgaa aattgagaat
    11641 tataaataat aatctgcgaa atctgcgtga actaaaattt ttatctgtga aatctgtgag
    11701 aaaaaaagga gcataaatga aaaacctggt taatcccttc aatccatttt gctatataat
    11761 tatagcgctt acattagttc tttgcttatc atcctgtaaa acgaagagtt ccaaacagga
    11821 acaggcagaa ttacaaaagg tttcggtcac tttagattgg actcccaata ccaatcatac
    11881 cggaatttat gtagctaaag aacttggcta tttccaggaa cagggcttgg aggtggatat
    11941 tttacagccg ggacaaaatg ttaccgacca aattgttgcc actggaaaaa gtgaatttgg
    12001 tgtaagctat caggaaaatg taattcgtgc cagaagtgaa aatattccgc tggtttcaat
    12061 tgcagccgtt attcagcata acacttccgg ttttgcctct ttgaaaaaag cgggaataaa
    12121 aagtcctctt gattttgaag gcaaaagata cggttcctgg gattcgcctt cagaacttgc
    12181 tatcctgaga gcaactatgg aaaaatatgg tgccgacttc aataaagtaa ccgttatttc
    12241 cggaatttac gatttctttt ccactattgg caaagatgct gattttgagt ggatttatta
    12301 tggctgggat ggagtggaag cggaaagaag aggcatagcc ctaaactata tacccctcaa
    12361 agaaatagac cctgtttttg actattatac acctgtaatt atcagcagcg aagattatct
    12421 ggcaaaaaat cccgagaccg ccaagaagtt tatgacagcc cttaaaaaag gttatgaata
    12481 ttgtattgcc aaacccgatt ccgctgccgc tattttgctg aaaaatgtcc ccgaacttaa
    12541 tgccgaacag gttaaacgta gtatgcaata tctggcaaaa gaataccaaa gcgatgctga
    12601 atcttgggga atacaaaagt tggctgtctg gaaaagattt tgtgaatgga tgtatcagca
    12661 acgtcttatt cagcttgcag ttgactataa caaggcattt acgaacgact atctacctca
    12721 ataaattcta tgcctaattg cattctggaa gcgaaggata ttcgtaaaac atttctcttt
    12781 aagggagcgc tggttgtggt tttggataaa ttacaattca gcatccagga aggtcagttt
    12841 gttagtattg taggtccaag tggttgtggt aaaagcactt tcctggaact tttggctgga
    12901 atcactaaac cagataatgg caatatttat cttgaaggca atgaaattac aggaaaaact
    12961 ggctttttgg gttatatgcc tcaagatgat ttgctttttc cctggctaac cacaatgcaa
    13021 aatattctac tgcccgtaaa agtgcaaggc aaagacgaaa aagaggcaag aaagaaaatt
    13081 attgaacttc tccctctgtt cggtttaatg aattatacgg atcatttacc ctaccagctt
    13141 tcggggggat taaaacaacg tgttgcgctt ttaagaacct atatgagcag tgcggaaatc
    13201 ttacttttgg acgaacccct ggcaaaattg gatgctttaa cgcgcagtca actacaaacc
    13261 tggctgaaag atattgtcca tttactaaat ctaaccgtta ttttagttac ccatgatatt
    13321 gacgaagcaa ttatgctttc ggatagaatt gacctgatga gtatcaatcc cggaactttt
    13381 atggaaagca tatatctgga tagaaatgca cccttaaacg ctgaagaaca gctaaaactg
    13441 aaaacaaaaa tacgcagtaa actggttaac ggagaagaaa aaagtg
//
LOCUS       DAOOZV010000051        13278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4662_length_13278_cov_66.022915, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000051 DAOOZV010000000
VERSION     DAOOZV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_13278_cov_66.022915"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(919..1539)
                     /locus_tag="PLW08_05960"
     CDS             complement(919..1539)
                     /locus_tag="PLW08_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPL52182.1"
                     /translation="MENLGKYFLELRLQREYSYQDIWNELRIPENQIKAIEENRFAEL
                     GPYGVAKTLVYNYARFLEADVDSVMNAFSSLMPESAKPEFQPATPVKEKKIMLSTNFM
                     WLIGILLIIIILGSILWYAYNQNWLKMPDFFASKKPDSTAVIIKEEKEETKPDTLRQR
                     LRAISESIPQTNSVSTPKSETKTVPPDTTDYIGNILGDSPVNVPIH"
     gene            complement(1527..2315)
                     /gene="dapF"
                     /locus_tag="PLW08_05965"
     CDS             complement(1527..2315)
                     /gene="dapF"
                     /locus_tag="PLW08_05965"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPL52183.1"
                     /translation="MLIPFLKMHAQGNDFVILDFFAPESFALAKLDFSSVAEELCKPH
                     FGIGADGLVLLMPNESCDAQMFIYNADGSTAEMCGSALRCVTWILHQKTGKKEFRVQT
                     EAGLKTCYVNEDNTITVNLGMPSMLKSAYQVMDFTGDLVEIGNQHFIVWVNSLQDDPH
                     LQYGSAIEHHKGFAQTVNVHFAVLLNRTAVAIKIWEKGCGATLACGTGASATVFSGIA
                     KGLLDNEVEVLMPGGIVKINASDNGYLLSGEVCEAFQGVYNWKI"
     gene            complement(2309..3058)
                     /gene="dapB"
                     /locus_tag="PLW08_05970"
     CDS             complement(2309..3058)
                     /gene="dapB"
                     /locus_tag="PLW08_05970"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPL52184.1"
                     /translation="MAVKICLLGYGKMGKMIAELASDFDCTVVSKIDPNQKDCYQEIS
                     EQSLQDADVCIDFSHPSVVLENIHKVLALKKNMVVGTTGWFEHLKEIQQQVQQAGTGF
                     LYGANFSIGMNIFKRLIAEATKAFDRFPEFDVYGWEMHHNQKADSPSGTAIELSKTIL
                     ANSSRKKTALFDPAYRKIEPEELHFGSLRAGSFPGTHIIGFDSATETIELSHCVRSRS
                     TFAYGALQAAKWIKDRKGFYSFAEMVEEVLC"
     gene            complement(3059..4183)
                     /locus_tag="PLW08_05975"
     CDS             complement(3059..4183)
                     /locus_tag="PLW08_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPL52185.1"
                     /translation="MKNDVVSYFLELVSIDSESGNERAIVDKLKKDLEELGAIVEEDD
                     CASKIEGNAGNLYAYFPGTINKKPILLCAHCDTVVPGKDIQPIMENGRIFTNGTTILG
                     GDDKSGIAEILMAIKQIKEKGIEHPPIEVLITVSEEIGLLGARNFDISKLQAGFGYAL
                     DTHQVGEVVIGAPSQISFQINFYGKEAHAGVEPEKGLNAIRLAAEAIAIMPNGRIDFE
                     TTCNLGIIKGGTATNIVPGLVTVEGEVRSHNKGKLQKVCEEIEQAVISTVHKFNANGA
                     KASFHFELKEEYEAFLIDRNAPVVTLAEKALQNLHLPADIKVGGGGSDANIFNANGLP
                     MIIVGTGMNNVHTTEEDILVDELYRGTAFVEELIHLYAEE"
     gene            complement(4419..5489)
                     /gene="rfbB"
                     /locus_tag="PLW08_05980"
     CDS             complement(4419..5489)
                     /gene="rfbB"
                     /locus_tag="PLW08_05980"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPL52186.1"
                     /translation="MNRVIVTGGAGFIGANYLHTLFADPSFTGKILNIDKLTYAGNLE
                     SLQDLEAKYGNSRYFFEQADICDAPKMKEIMHQFQPDTIVHFAAESHVDRSIDGPMEF
                     VQTNLVGTATLLNSALEYYRTLDEEAKKHFRFHNVSTDEVFGSLGENGMFTEETPYDP
                     SSPYSASKAGSDHLVRAWQRTFGLPITISNCSNNYGPYQFPEKLIPLMILNCLAHQPL
                     PVYGKGINVRDWLFVTDHCEAINIIIRKGKVGETYNIGGHNEMKNIDIVTTICKILDE
                     MEPSKKLKSYTELITFVQDRPGHDLRYAIDATKIEKELGWKPAETFATGIRKTVAWYL
                     ENKQWWQNIQNNKYRQERLGIG"
     gene            complement(5486..6343)
                     /locus_tag="PLW08_05985"
     CDS             complement(5486..6343)
                     /locus_tag="PLW08_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPL52187.1"
                     /translation="MNEHLGRGLSALIPDNDREQTPQLGITTLPIASIKPNRYQPRKT
                     FDPEKMAELTESIKENGIIQPLIVTKTSSSEYELVAGERRLEAAKQAGLEKVPVVIRS
                     VSKKEQLQLALIENIQREDLNPIEEAIAYNTLVEDFGLTHNQISEIVGKDRATVTNSL
                     RLLKLPEEVKQMLVSGELNSGLARTVLSVPPELQNSFAEYIIKYNLTVRQAEEKAKTF
                     SLANKEEPEKAKTNARTKELERELSNLFCLKVKVQENNGKGKITLVYKSPEELEAFRT
                     TLNKIRQEK"
     gene            complement(6355..7119)
                     /locus_tag="PLW08_05990"
     CDS             complement(6355..7119)
                     /locus_tag="PLW08_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL52188.1"
                     /translation="MPKVITVVNQKGGVGKTTTAVNLSAGLAVLEKRTLLIDFDPQGN
                     ATSGVGIDKDKVELQIYDALIGRAPIEKTILSTATKNLFCIPGNINLTGAEIELVHEF
                     AREQKLKEALQPILNTFDYIIIDCPPSLGLLTVNAMTAAMEVLIPIQCEYYALEGVSQ
                     LLTTIRLIQKNLNPGLNILGVLLTMFDKRVNLSLQVAKEVHRYFKEKVFRTIIPRNIK
                     LTEAPGFGKPIFLYDIRSPGAMSYLNLANEVINRSE"
     gene            complement(7144..7785)
                     /gene="rsmG"
                     /locus_tag="PLW08_05995"
     CDS             complement(7144..7785)
                     /gene="rsmG"
                     /locus_tag="PLW08_05995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPL52189.1"
                     /translation="MTEEKQIFEDYLRFLGLEDIDHLMARFEHYLHLLYNANRKVNLV
                     SRNIPLERYWVQHFLDSIVALECLDFTDKTVLDFGSGGGLPGIPLKLAVPDFNLTLLD
                     SVQKKARVLREFVEALSLSATNVEASRLEDYAYVAKRPSYDFIVCRAVALEERYFSPL
                     RRLLKPSGMVVFYKAQKLDDVQVLKYEILLDKDYPALGQRKLIGIKQRDLLFH"
     gene            complement(7782..10352)
                     /locus_tag="PLW08_06000"
     CDS             complement(7782..10352)
                     /locus_tag="PLW08_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPL52190.1"
                     /translation="MRKKVLTAFMLGFMLISTLMGENISSPILSQTKSDTLYLQDFSL
                     LSEDDFRELENLLKEQQLNPQDLNFEKDWDLSTWGKSKSFLRSLQEPYYGLGLMQEIR
                     EAANKANTDEGFAFLVDLLYKNATDGAGSFPLYLNYKDLYQQEFNKKVKQPKDLFRFY
                     EKALKDIIPVLANAYSHYNLDLWNKYVFYLFVALRENEDSEAYNAYFNKHNIPLWEEI
                     EDQEIKELMELFQEADVLKAAMQFLALCDVIKNEAGKLNYTNKKPIIQECEFGTMVIG
                     TEKDDVYNAKMFKKPLILLIEPAGNEKYTMTLATGAKNYAYLLIDYKGNDSYVNPDIG
                     SLFFALSGLGISYDLEGNDIYRTGDFSFAGMMGIQLHRDFQGTDIYESGFFSQGAAIR
                     GLSILQDMQGNDIYSATCLAQGFGSIRSAGVLLDFDGADNYLLGGKYFHSPLMPNDYL
                     TLGQGVGFGLRPDFAGGLGLLFDKNGNDRYLGGVYAQGVGYWYALGMLIDEAGNDVYN
                     AVYYPQGSGIHLASGFLFDGSGDDAYYSRHGPGQGAGHDWGLGIFIDAKGNDAYSIEG
                     GNGLGLSNSVGIFIDKGGNDRYERNNPQNYGNAVYSRATGGIGLFLDKDGKDSYPDTL
                     MAEGATWKKGTYGIGRDLDSGKLMSSSTTDSETKEPAEIPMLPPPSPDEPIADIFSAA
                     AEWEVGNAIERVKKAREIMLARADEAIDYVIKNKLDTKSGLEYRALEVLVKENKQFKQ
                     LLFDYTDDIDSLKAKNALSLIAGVGDSTLIEPLRKHLNNGKYITTCLSLLGSIKCAES
                     VDLLFQYAFHPSERYRYIVARSLKQIGTPEAHNCLKQMANDNSFLVKTLVSKWEEEQQ
                     "
     gene            10477..12846
                     /gene="priA"
                     /locus_tag="PLW08_06005"
     CDS             10477..12846
                     /gene="priA"
                     /locus_tag="PLW08_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPL52191.1"
                     /translation="MHYYVIHLPLEIPKELIYCSSAEIVEGARVIVNLSGRLWTGICG
                     AKTRPEEDKDIRYKAVIEVLDTVPVLSPELMKLGYWMADYYHCSVGKALFAMLPARLL
                     PEMEANVIWLSENVPEPFIPLKKAIDKSENPVLKTLRKELTSYPLYKRIEEGEELGLL
                     KIERRLNHKDKPRTVNFIIRLQPTLEEDTLPLKQREAWEKIKEEEPSFPMSNISSTIS
                     YSAIKALVKKGFIKIEPRRFDPESISFENSSPLKQIALNYAQVEAIKDITEGFGTFNV
                     NLLYGITGSGKTEVYIELIRHYLSVNKTVIFLIPEIALTPQMVDRFQSNFGQILAIQH
                     SQLTEKDRLIQWQKIKSGEKRIVIGARSAVFAPLPDLGLIIVDEEHEGTYKQESAPRY
                     QGRDLAIVRAQIQNAQVILGSATPALESWYNALNGKYKLQKIESRPLDYCLPDVQVVD
                     LRDEYEQSLISAPLLEAIDKRLQRKEQVILFQNRRGYSSFMQCLKCGKLITCKNCEIS
                     MSYHRDREEMQCHYCGNTIPSPRKCPYCGSYSFAYGAPGTQKIEQNLKILFPEAKILR
                     MDSDSARKRDTYKSMYNRMKNKEVDILLGTQMISKGLDFPLVTLVGIVMADISLNLPD
                     FRAAERTFQLLTQVAGRSGRRDKAGEVIIQTYNPQHYAIESASQQNYIRFATEELGYR
                     KKLYYPPYYRLARILFQSTDLETLKKEMSNMREVLYLLRKQFPMPQLLLLGPSVAPFS
                     KISSYYRYHLIFKAQTVKILQNAIRLFNSLYNCPSNIRKQIDIDPNTLM"
BASE COUNT         4042 a         2814 c         2410 g         4012 t
ORIGIN      
        1 accttaaagc ccggagggcg acacaatgat agcgatgggt gcgtaagccc ctcggaaaat
       61 gtaagccccg agttcctttt tccttttatc ttcccctttt ccaaaagccc gtcagggcga
      121 tacaaggatg tagaaggtgg cgtaagcccc tcgtaaaatt cacgatttaa tcggtggaaa
      181 gttttgactt acaattttca cttgccttca atattcccac accccacact cttttctcac
      241 ttacaaccaa tctgcaactg gttaataaat ctgagaaatt gtgagtcaaa acaaaacata
      301 atttaaaacc ggatcggcac atcatttctt tgcagaaaga actggctaac tcaaatcaca
      361 gcttgccatt agcacacgga ataaatatca ttcccaaaga aatcccaaaa ttatttcaat
      421 aacactccga tatgactccc atatcattcc catatcgcga tatgggaatg atatgggaat
      481 ctcatccgct gcataacgca aaaaagcaga aattcataca caactatttt gtttctgttt
      541 taactcacat tattttggtt tggagaccaa taaaaactgc ggttgtatgt taaaaatgat
      601 tgcagggtgt gggaatattg aaggcaggtg aaaattgtaa gtcaaaacct cccactgttt
      661 acatcgtgaa ttttccgagg ggcttacgcc accttctaca tcattgtgtc gccctaaggg
      721 gcttattttt ccagatatgg tgaaaaaagg aactcggggc tttcattttc cgagggggtt
      781 acacacccat cgctatagtt gtgtcgccct ccgggcttta atgaaatctc aaccttctaa
      841 accttctcaa ccctctaaac cttattttcc gaggggctta cgccaccata gctatcattg
      901 tgtcgcagag gcaagaattt aatggatagg cacattgacc gggctatcac ctaaaatatt
      961 gccaatgtag tcagttgtat ccggtggaac agtttttgtt tcggatttgg gagtagatac
     1021 actattagtt tgagggatgg attcactgat agcacgcaag cgttgacgca aggtatctgg
     1081 tttggtttct tctttttctt ctttaattat aacagcagtg gaatcaggtt ttttagaagc
     1141 aaaaaaatcg ggcattttca gccaattttg attgtaggca taccataaaa tagaaccgag
     1201 aataataata atcaataaaa tgccaatcag ccacataaag ttggtggaaa gcataatctt
     1261 tttttctttc acaggagttg caggttggaa ttccggtttg gcactttccg gcattaaact
     1321 ggaaaaagca ttcattacgg aatccacatc cgcttccaga aaacgggcat agttatagac
     1381 caatgtttta gcaactccat agggaccaag ttcggcaaag cgattttctt caattgcctt
     1441 tatctgattt tccggaatac gcagttcatt ccagatatcc tgatagctgt attcacgctg
     1501 taaacgcaat tccagaaaat acttgcctaa attttccaat tgtaaactcc ctgaaatgcc
     1561 tcgcagacct ctccgcttaa taaataacca ttatcggaag cattgatttt aactattcct
     1621 ccgggcatta agacctcaac ttcattatct aataagccct tggcaatacc gctaaaaact
     1681 gtagcggaag cacctgttcc acaagccaga gtagctccgc aacctttttc ccagattttg
     1741 atggctaccg ctgttctatt cagtaaaacg gcaaaatgca catttaccgt ttgtgcaaat
     1801 cccttatgat gttctatggc agaaccgtat tgaagatggg gatcatcttg caaagaatta
     1861 acccaaacaa taaaatgctg attgccaatt tccaccaagt cgccggtaaa atccattacc
     1921 tgataggcac ttttcagcat tgaaggcatt cccaggttaa cggttatagt gttatcctca
     1981 tttacataac aggttttaag tcccgcctca gtttggactc taaattcctt ttttccggtt
     2041 ttttgatgca aaatccaagt tacacagcgt aatgcagaac cgcacatttc agctgttgag
     2101 ccatctgcat tgtaaataaa catttgcgca tcgcaggatt cattaggcat aagtaaaacc
     2161 agtccatccg ctccaatgcc aaagtgcggt ttacaaagtt cttccgctac agaagaaaaa
     2221 tccagctttg ccagagcaaa tgactccgga gcaaagaaat ccaagataac aaaatcgtta
     2281 ccttgagcgt gcattttgag aaaaggaatc agcacaatac ttcctctacc atttccgcaa
     2341 aactgtaaaa tcccttgcgg tctttaatcc attttgctgc ctgtaacgct ccgtaggcaa
     2401 aagtgctacg cgaacggaca caatggctaa gctctatagt ttcggttgca ctatcaaaac
     2461 caattatatg cgttccggga aaagaaccgg cgcgaagtga gccaaaatgt aattcttcag
     2521 gttctatttt acgataagca gggtcaaaaa gagcggtttt tttcctgctg ctgttggcta
     2581 aaatagtttt agaaagttca atggcagttc ccgaaggact atccgccttt tgattatggt
     2641 gcatttccca gccgtaaaca tcaaattccg gaaaacggtc aaatgcctta gttgcttctg
     2701 caataagtcg cttaaagata ttcattccga tagaaaaatt ggctccgtaa agaaaacctg
     2761 ttcctgcttg ctgaacctgc tgttgtattt cttttaggtg ttcaaaccag ccggttgtgc
     2821 caactaccat atttttcttg agagcaagaa ctttgtgaat attctccaga actacagaag
     2881 gatggctgaa atcaatacaa acatctgcat cttgcaggga ctgctcactt atttcctggt
     2941 agcaatcctt ttggttaggg tctatcttag aaacaacggt gcaatcaaaa tcagaagcca
     3001 attcggcaat catttttccc attttgccat aacctaacaa acagatttta acagccattt
     3061 attcctccgc ataaagatgg atgagttctt ctacaaaagc agttcctcta tagagttcat
     3121 ccaccagaat atcttcttcc gtagtatgca cattattcat tccggtgccg acaataatca
     3181 ttggcaagcc gttggcatta aaaatattag catcacttcc accacctcca actttgatgt
     3241 ctgccggaag atgtaaattc tgcaatgcct tttctgcaag ggttactacc ggagcattac
     3301 gatcaattaa aaatgcctcg tattcctctt tcagttcaaa atgaaaggat gctttggctc
     3361 cattcgcatt aaatttatgg accgtagaaa taactgcctg ttcaatctcc tcacagacct
     3421 tttgcagttt tcccttatta tgactgcgaa cttcaccttc aacagttact aacccgggaa
     3481 caatattggt agctgttcca cctttaatga taccgagatt acaagtagtt tcaaaatcaa
     3541 ttctgccatt aggcataatt gcaattgctt ctgcagccag gcgaatggca tttaatcctt
     3601 tttcaggttc tacacccgca tgcgcttcct tgccataaaa gttaatctga aaagaaattt
     3661 gagaaggcgc tccaataacc acttccccaa cctgatgagt atccaatgca taaccaaaac
     3721 cggcttgcag cttggaaata tcaaaattcc tggcgcccaa aagccctatt tcttcggaga
     3781 cagttatcaa aacctcaata ggaggatgct caataccctt ttccttaatt tgtttaatag
     3841 ccataaggat ttccgcaatg ccggatttat catctccgcc taaaatagtt gtgccattag
     3901 taaaaatgcg accgttttcc ataattggct gaatgtcttt gccgggaact acagtatcac
     3961 aatgagcaca aagaaggatt ggctttttat tgatagttcc gggaaaatag gcatagagat
     4021 tacccgcgtt gccctcaatt tttgaggcgc agtcatcttc ttctacaata gcaccaagtt
     4081 cttccaaatc cttttttagc ttatccacaa tggctctttc gttaccggat tcgctatcaa
     4141 tagagacgag ctcaagaaag tagcttacta catcgttttt cattgggact tccttttata
     4201 cattccttac taaatatata atagccaaaa tttcggtata tagcaactat ttattctatg
     4261 gattttttac accgaaacca ccgaaatcat tgagaacacc aagtagaaag taaaaacagc
     4321 aaaaatctgg gttgccaata tatggctctg ctgattttcc gaaagggtta cctcctggtc
     4381 aacttatagg aaactacagg gagttctttc atcctgtttt atccaattcc caggcgttct
     4441 tgacggtatt tattattttg aatattttgc caccactgtt tgttttccag ataccaggca
     4501 actgtttttc tgattcctgt ggcaaatgtt tcggcaggtt tccaaccaag ttctttttca
     4561 attttagtgg catcaatggc atagcgtaaa tcgtgacctg gtctatcttg cacaaaagtt
     4621 atcagttccg tatagctttt cagtttcttt gagggctcca tctcatccaa aatcttgcag
     4681 atagtggtaa caatatcaat attcttcatt tcattatggc cgccaatatt atatgtctcg
     4741 cctacttttc ctttccgaat aataatgttg atggcttcac aatggtctgt aacaaaaagc
     4801 cagtcccgca cattaattcc ttttccgtaa acaggcagtg gctgatgagc gaggcagttc
     4861 aaaatcatta aaggaatcag cttttcagga aattgataag gtccatagtt attggagcaa
     4921 ttggagatgg tgattggcag tccgaaagtt ctttgccagg cacgcactaa atggtctgaa
     4981 ccagcttttg atgcagaata gggtgaagaa ggatcgtaag gagtttcctc tgtaaacatt
     5041 ccgttttccc ctaaagaacc aaaaacctca tccgtagata cattatgaaa acgaaagtgc
     5101 ttttttgctt cttcatccaa agtgcgatag tattccaaag cggaattcag caaagttgcc
     5161 gttcctacta aattggtttg gacaaattcc ataggaccgt caatacttct atccacatgg
     5221 gattcggcag caaaatggac aattgtatcc ggctgaaact gatgcataat ttctttcatt
     5281 ttgggggcat cgcagatatc tgcctgctca aagaaataac gcgaattacc atacttggct
     5341 tccaaatcct gcaagctttc caaattacct gcgtaggtaa gtttatctat gttcaatatc
     5401 ttaccagtaa aagaagggtc tgcaaaaaga gtatgtaaat agttggcacc gataaaacca
     5461 gctccaccgg taacaataac tctgttcatt tttcctgcct tattttattt agggttgttc
     5521 taaatgcctc cagttcttct ggagatttat acactaaagt aattttgcct ttgccattat
     5581 tttcctgcac tttcactttc aggcaaaaca aattgcttag ttccctttcc agttctttag
     5641 tgcgggcatt tgtttttgcc ttttccggtt cttccttatt agcgagagaa aaggttttcg
     5701 ctttttcttc tgcctgacgc accgttaaat tatactttat tatgtattcc gcaaagctat
     5761 tttgcagttc cggaggcaca gataaaacag tgcgagccaa tcctgaattc aattctccgg
     5821 aaacaagcat ttgttttact tcttcaggaa gttttagcaa acgcagggaa ttagtgactg
     5881 tagctctgtc cttaccaaca atttcagaaa tttggttatg cgttaaacca aaatcctcaa
     5941 ccagagtatt ataggcaatt gcttcttcaa taggattcaa gtcctcccgt tgaatgtttt
     6001 ctattagagc gagctggagc tgttcttttt tagaaacgct gcggataaca acaggcactt
     6061 tttccagtcc tgcctgtttt gctgcttcca gtcgtctttc acctgccaca agttcatatt
     6121 ccgaagagct tgttttagta acaatcaggg gttgaataat accgttttct ttgatggatt
     6181 cagttaattc cgccattttt tccgggtcaa atgtttttcg gggttgatag cgattgggct
     6241 taatacttgc aataggcaaa gtagtaattc ccaattgagg agtttgttct ctatcgttat
     6301 ccggaatcag agcggaaagt cctcttccca aatgttcatt cattttttat taccttattc
     6361 gcttctattg ataacttcat ttgccaaatt taggtaactc atcgcgcctg gagaccttat
     6421 gtcatagaga aatataggtt taccaaaacc aggagcttca gttaatttaa tattccgggg
     6481 aataatagtg cggaagacct tttccttaaa atagcgatga acctctttag caacttgcag
     6541 agagaggttt acccttttat caaacatcgt taacagcaca cccagaatgt ttaaaccagg
     6601 gtttaagttc ttttgaatca agcgaatggt tgttagcaac tgactaactc cttccagagc
     6661 ataatattcg cattgaatag gtatcaagac ctccattgcc gcagtcattg cattcaccgt
     6721 taataaacca agtgaaggag gacaatcaat tataatgtaa tcaaaagtgt ttaaaatggg
     6781 ttggagtgct tctttcaatt tctgttcgcg ggcaaattca tgcaccagtt caatttccgc
     6841 tccggttaaa ttgatgtttc ccggaatgca aaaaaggttt ttggtggctg tggaaagaat
     6901 agttttttct ataggagctc tgcctataag agcatcataa atttggagtt ccactttatc
     6961 tttatcaatt cctactccgc tggtagcatt accttgagga tcaaagtcaa tcagcagagt
     7021 ccttttttcc agaactgcca aaccggcaga aagatttacg gcggtagttg tttttcctac
     7081 accacctttc tggtttacaa cagttattac cttgggcata tatatccttt aatttttaag
     7141 aacttaatga aagagaaggt cacgctgttt gataccaatt aatttgcgtt gacctaaggc
     7201 aggataatct ttatccaaaa gaatctcata ttttagaact tgcacatcgt ctaatttctg
     7261 tgccttgtaa aaaacaacca ttccgcttgg tttcagcaat cttcgcaaag gagaaaagta
     7321 tctctcttcc aaagcaacgg ctctacaaac aataaaatcg tagctgggac gctttgccac
     7381 ataagcatag tcctctaaac gactggcttc cacatttgta gcagaaagag ataaggcctc
     7441 cacaaattca cgcaaaaccc tggctttttt ctgcaccgaa tccaaaagcg tcaaattaaa
     7501 atccggaaca gcaagtttca gaggaattcc tggtaaacca cctcccgaac caaaatccaa
     7561 aacggtttta tctgtaaaat ccaaacattc caaagcaact atgctgtcaa ggaaatgctg
     7621 aacccaatat ctttccagag gaatgtttcg ggaaacgaga tttactttgc ggttggcatt
     7681 ataaagtaaa tgcaggtaat gttcaaaacg agccattaaa tggtctatat cctccaaacc
     7741 gagaaagcgc agatagtcct caaaaatttg tttttcttcc gtcattgttg ttcctcttcc
     7801 catttactaa ctaaggtttt caccagaaag gaattgtcat ttgccatttg cttcagacag
     7861 ttatgcgctt caggcgttcc gatttgtttt aaggaacggg ctacaatata gcgataacgc
     7921 tctgaaggat gaaaagcata ttggaatagc aaatcaacac tttccgcaca ttttatgcta
     7981 cccaaaagcg aaagacaggt ggtgatatat tttccgttgt ttaaatgttt acgcaagggt
     8041 tcaattaaag tgctatctcc aactccggca attagagaca gggcattttt agctttcaaa
     8101 ctatctatat catcggtata atcaaaaaga agttgtttaa actgcttatt ttctttcacc
     8161 agaacctcta aagctctata ttccaatccg gactttgtat caagcttatt ctttatcaca
     8221 taatcaattg cctcatcagc tctggcaagc ataatttccc gggctttttt cactctttca
     8281 atagcgttgc caacttccca ttcggcagca gcagaaaaaa tatctgctat tggctcatcg
     8341 ggagaaggag gaggcaacat tggaatttct gccggttctt ttgtttcaga atcggttgtt
     8401 gatgaggaca tcaactttcc ggaatccaaa tctctgccaa tgccataagt gccttttttc
     8461 caggttgctc cctcagccat caaagtgtcc ggatagctat ctttgccatc tttatccaaa
     8521 aaaagaccta taccaccggt tgctctgcta tagacagcat taccataatt ttgaggatta
     8581 ttgcgttcat aacgatcatt tcccccttta tctataaaaa ttcccactga attacttaaa
     8641 cccaaaccgt ttcctccttc aatggaatag gcatcattcc ctttagcatc aataaaaatt
     8701 cccaaacccc aatcgtgacc tgcaccttga ccaggaccgt gtctgctgta atatgcatca
     8761 tctccactgc catcaaaaag aaaaccagaa gcaagatgaa ttccagaccc ttgaggataa
     8821 tatacagcat tgtaaacatc atttccagct tcatcaatca acattcccaa agcataccaa
     8881 tagccaactc cttgtgcata aacacctccc agataacgat cgttgccatt tttatcaaaa
     8941 agaagcccta atccaccggc aaaatcggga cgcaaaccaa aaccaacacc ttgtcccaga
     9001 gtaagataat cgtttggcat caagggtgaa tgaaagtatt ttccccctaa aagataatta
     9061 tccgctccat caaaatccaa taaaactcct gcagaacgga tagaaccaaa accctgagcc
     9121 agacaagtag cgctgtaaat atcatttccc tgcatatctt gaaggatgga aagaccgcgt
     9181 atagcggcac cttggctgaa aaaaccgctt tcataaatat ctgtaccttg aaaatcacga
     9241 tggagttgaa ttcccatcat tcccgcaaaa gaaaaatcac ctgttctgta aatatcattt
     9301 ccttccaaat cataagaaat tcctaagccc gaaagagcaa aaaacaaact tccgatatca
     9361 gggttcacat aactgtcatt tcctttataa tcaatcagca gataagcata atttttagcc
     9421 cccgttgcca aagtcattgt atatttttca tttcctgcgg gttcaataag taaaataagc
     9481 ggctttttga acattttagc gttataaaca tcatcttttt ccgttccgat aaccatagtt
     9541 ccgaattcgc attcttgaat tatgggcttt ttattagtgt agtttaattt acctgcttca
     9601 ttttttatta catcgcatag agccagaaac tgcatagcgg cttttaaaac atcagcttcc
     9661 tgaaaaagct ccattagttc cttgatttcc tgatcttcta tttcttccca aagaggtata
     9721 ttatgcttat taaaataggc attataagct tcgctatcct cattttccct taaggcaacg
     9781 aaaagataga aaacatattt gttccataaa tcaaggttat aatgcgaata agcattcgcc
     9841 aaaacgggaa taatatcttt taatgctttt tcataaaagc ggaacagatc tttgggctgt
     9901 tttacttttt tattgaattc ctgttgatat aagtccttgt aattcagata gaggggaaat
     9961 gaacctgcac catcagtcgc atttttatac agtaaatcaa ccagaaaagc aaagccctca
    10021 tccgtattag ctttattagc tgcttctcta atttcctgca ttaaacccaa accgtaatag
    10081 ggctcttgca aagagcgtaa aaaacttttg cttttacccc aggtagagag gtcccaatcc
    10141 ttctcaaaat tcaggtcttg agggtttaat tgttgttctt tcaataaatt ttccagctcc
    10201 ctgaaatcat cttcggaaag cagggaaaaa tcctgtaaat ataaggtatc ggattttgtc
    10261 tgggagagaa taggagaaga tatattttct cccattaagg ttgaaataag cataaaaccc
    10321 aacataaatg ccgttaaaac ctttttcctc atattatcct cttcactgtt tcaatttatt
    10381 ggaactttgt tttattgtca atctttagat tttactccaa gacctccaga ccttttttgg
    10441 ggttgacaag ctatagccaa acaaaagttt cgtcttatgc attattatgt aattcatttg
    10501 cctttggaaa tacccaagga actgatttat tgttcatccg cagaaattgt ggaaggagca
    10561 agagtaatag taaatctttc cgggcgttta tggacaggaa tatgcggagc aaaaacccgt
    10621 cccgaagagg ataaggatat tagatataag gcagttatag aggttttgga tacagttccc
    10681 gtgctttcac cggaattgat gaaattgggt tattggatgg ctgattacta tcattgttca
    10741 gttggcaagg cacttttcgc gatgttgcct gcgcgtttgc tgcctgaaat ggaggcaaat
    10801 gttatttggc tttcagagaa tgttccggaa ccttttatac ccttaaaaaa ggcaatagat
    10861 aaaagtgaaa atcctgttct taaaacatta cggaaagagc tgacttcata tcctctctat
    10921 aaaagaatag aagagggtga agaactggga ttgctgaaaa tagagagaag attaaatcat
    10981 aaagataagc cccgaacagt aaattttata atccgtttgc aaccaacttt ggaggaagat
    11041 actttaccct taaaacaaag agaggcatgg gaaaaaatta aagaggaaga accctctttt
    11101 ccaatgtcca atataagttc cacaatctca tacagtgcaa ttaaggcatt agttaaaaaa
    11161 gggtttatta aaatagaacc acgccgtttt gatcccgagt ccatttcttt tgaaaattct
    11221 tcacctttaa aacaaattgc cctcaactat gcccaagttg aagcaattaa agatattaca
    11281 gagggttttg gcacttttaa tgtaaacttg ctgtatggca ttaccggaag tggcaaaaca
    11341 gaggtctata ttgagcttat caggcattat ctatccgtaa acaaaaccgt tatttttctg
    11401 attccggaaa ttgctctcac tccgcaaatg gttgatcgtt ttcagagtaa ttttggacaa
    11461 attctggcaa ttcagcatag tcagctgact gaaaaagacc gtttaatcca gtggcagaaa
    11521 ataaaaagcg gagaaaaaag aattgttatc ggagcgcgca gtgccgtttt tgctccttta
    11581 cccgatttgg gcttaattat tgtagatgaa gaacatgagg gaacttacaa acaggaaagc
    11641 gcacctcgtt atcaaggcag ggatttagct atcgtaagag ctcaaataca aaatgcacaa
    11701 gtaattttag gtagtgctac accagctttg gaatcctggt ataatgctct aaacggaaaa
    11761 tataaattgc aaaaaataga aagccgtcct ttggattatt gtctgccaga tgtgcaagtt
    11821 gttgatttaa gagatgaata tgaacaaagt ttgatttccg ctcctctttt ggaagcaata
    11881 gataaacgcc tccaaagaaa agagcaggtt atcctttttc aaaatcgcag gggttattca
    11941 tcttttatgc aatgcctgaa atgcggaaaa ctaattactt gcaagaattg tgaaatcagt
    12001 atgagttatc atcgtgaccg cgaagaaatg caatgtcact attgcggaaa cacaattccc
    12061 agtccgcgta aatgtcctta ttgtggctct tattcttttg cctatggagc tccgggaact
    12121 caaaaaattg aacaaaactt aaaaatcctg tttccagagg caaaaatctt aagaatggat
    12181 tcggattcgg ctcgcaaaag ggatacctat aaatcaatgt ataaccgtat gaaaaataag
    12241 gaagtggata tattactggg aacgcagatg atttccaaag gtctggattt tcctcttgta
    12301 actttggtag gaattgtgat ggctgatatt tccttaaatt tgcccgattt tagagccgca
    12361 gaaagaactt ttcaattgct tactcaggtt gcaggacgct caggacgcag agataaagcg
    12421 ggagaagtta ttattcaaac atataatccc cagcattacg ctattgaatc agccagtcaa
    12481 caaaactata ttcgttttgc cacagaagaa cttggttacc gaaaaaagct ctattacccg
    12541 ccctattacc gtttagccag aattttgttt caaagcaccg atttggaaac cctgaaaaaa
    12601 gaaatgagca atatgagaga agtcctttat cttttacgca aacaatttcc tatgccacaa
    12661 ctattattac ttggtccttc agtggctcct ttcagcaaaa tcagttctta ttaccgctat
    12721 catcttattt ttaaagccca aaccgtaaaa attctgcaga acgctatcag attatttaat
    12781 tccctttata attgcccttc caatatccga aagcagattg atattgaccc caataccctg
    12841 atgtaatata aaatgcggag tggtaacaac attatacccg aaaatatatg ttcatttttg
    12901 ttcactccac aagggaaaat atatttaaat tgtaacaatt ctacaatgca aaacaaatat
    12961 agcaatggtg gcgtaagccc ctcagaaaaa agggttgaga gggttgagaa ggtttatatg
    13021 gttgagattc cttaaaagcc cggagggcga cacaatgata gcgatggtgg cgtaagcccc
    13081 tcggaaaatg aaaactctga cttctttttc tttttctctt tcccactaaa aaaagcccct
    13141 aagggcgaca caatgatagc gatgaaggcg taaacccctc gggaaaatgc gattatttcg
    13201 gtaacgaaat agaggttgaa aggtagttga ccaggaggtc aaacttccat tttaaaaatc
    13261 attataactc ctaaatat
//
LOCUS       DAOOZV010000052        12900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_4821_length_12900_cov_62.471779, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000052 DAOOZV010000000
VERSION     DAOOZV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_12900_cov_62.471779"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /gene="hutH"
                     /locus_tag="PLW08_06010"
     CDS             <1..1502
                     /gene="hutH"
                     /locus_tag="PLW08_06010"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPL52192.1"
                     /translation="DGNSLTLEAVEQVACYNTPIRLSDSSKGKVAKCRAYVDKIIESG
                     EIVYGLNTGFGKFSTVNIPEENIAELQLNLIRSHSTNIGEPYSEVQTRAIMLLRIAVL
                     AKGHSGIRLETLETLVQMLNKGIQPIIPMRGSVGASGDLSPLSHLALVLIGEGEAIYK
                     GERLPGASAMQKAGINPVQLAAKEGLALNNGTQVMTALGVLNLLEAERLCRYADIIAA
                     MSIDALKGTPKAFDELIHNLRPHPGQKISAKNIRTLLEGSPLRESHRNCGNVQDAYSL
                     RCTPQVNGAVRDACSYVRRILEIEINSATDNPLIFPDEGKVISGGNFHGEPLAIALDT
                     LGLAISELASISERRIEQMLNPALSRGLSPFLATRPGIDSGFMIQQMTAASLVSENKV
                     LAHPACVDSIPTSANQEDHISMGSISAIKLCQIVQNVASVLGIELLIACSALDQRQIS
                     TSPVLEKVKSALRENVAPLQGDRVMYPDVNKAIELVRSKRLDEICPEIG"
     gene            1534..4038
                     /locus_tag="PLW08_06015"
     CDS             1534..4038
                     /locus_tag="PLW08_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL52193.1"
                     /translation="MKFRDIIICLLCLSGIFACEVNTLFNARNYFKSAQARPLTSNGR
                     PNAQAIDEYTKAIKKCGKIISTDKKGKRVEEAYYLMAKSLYYKGNSAFQAKDQFQNLV
                     IRFPDSKYVPEAYIYIAKILRETNQPKEAEKLLDEFLRNPKYRKHHPEALFVLADFAI
                     KDKDFIKAQHYLERIITEFPKTKEYREAYFLFGKNYYEQKDYDKSLEAFKKMQKARGI
                     DKTIKLEGTYYIGLNELELGQAEKALKTAKGLIKSESRPDKIPFVRLLKARAQFALGD
                     TTEARTEIEFITKNYPRTEGSAGAYYYLAEYYYYNLGDIPKAVLNYNRVRTEFSGSPL
                     AQTAQAKAVALGFVAPRTGLDCETGLSQFLDYHYQAAESFLNVLSLPDSAIARYKIVI
                     SQKDTLTARRDSLQTAINRNNAILDSLRALLPPPEIPAIDSLGLLEQAELPVTQTDIT
                     SESAFADTLSETIISSPDSLQTFADTLQITESEIPVADSLFVTETISEGDTLATEEEK
                     NKDNGLPDSSLAEEKVELSSPFTETTEPDSLTLAIAKHIQDLEKENARYSQRLEKLEE
                     IIERFESEIVPFCMFAIGSIYHNRSPESPENSKIMAKMQEEYPENKFTKALYALQNNL
                     PVRLIDPKEEREEQLLDMYFEQIYTAPDSALAGLQKLTNSPYHKIKLAANYRLGWYYS
                     FEQIDTLLAKPYLKAVLDEPEAGEYAVVARRFFDGNKFLLRGSFPVVIPQTDTTAVDS
                     TGADIEESIKEWFFPAPAEIYETLPDSLKIYSGIPMLMDSLVKAVSPPEDTTEEKNIS
                     PEMETLDDNIPPPEKNPVPALPEELIPEKKEELPLE"
     gene            4035..4673
                     /locus_tag="PLW08_06020"
     CDS             4035..4673
                     /locus_tag="PLW08_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52194.1"
                     /translation="MKWLNHPLSGQHFYLKSAVFFTGLAIGLDSSLRQLLIQLVLILT
                     ILIFEPKLYLSLLNALKKLLPFFAGYWVFATLFGQEFLTTVFFSVQILYLIAVTVYVL
                     GNVKMEQIASQSRWIRKWKPVNSLFYYLFATVFFTRSFFAEYRKLKLVKENSFSLAQL
                     LDVLANVSALSPQISNSVTNMLKFGGEEKTSNPQANYLGIFFLALLVIVHGF"
     gene            4685..5503
                     /gene="truA"
                     /locus_tag="PLW08_06025"
     CDS             4685..5503
                     /gene="truA"
                     /locus_tag="PLW08_06025"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPL52195.1"
                     /translation="MARYLIKICYDGTGYCGWQKQKNGLAIQEVMEKTLEIFVGKKTS
                     LIAAGRTDAGVHSLGQYAHFDYSGSMQEKQLLLAFNRYLPDSIKVLDIMKVSPCLNAR
                     YQAYERSYRYRLAKEKTPFNRNYSGFLPHLHLNLEKMQSAIQYLLGKHDFSTFGKSNP
                     EVPNRICELKHVSISETEQYFVFDFTADRFLHNMVRRIVGTLANISHLHLPQETILEI
                     LENRCPRQNLVITAPASGLYLMDVKYPAKFLDESYNPNFEENGMVNNPLLHSIK"
     gene            5522..6781
                     /gene="hisS"
                     /locus_tag="PLW08_06030"
     CDS             5522..6781
                     /gene="hisS"
                     /locus_tag="PLW08_06030"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPL52196.1"
                     /translation="MKYNIPRGTFDILPEDSYKWQFLQDSFRKMATCFGYEEITTPIF
                     EVAELFERSSGESSDVVQKEMYRFQDKKGREFALRPEGTAPVVRSYVENHLDKLSSRT
                     KLFYLGPMFRYDRPQAGRYRQFYQYGIEFIGSNNPYYDAEVIAVAFLWLNKLGLNNLR
                     LEINSVGCPDCSAVYDKALLEYYRPFLPKLCPDCVKRLETNPRRLLDCKVPSCIEFRR
                     NAPSQMDYLDESCKKHFSAVCDYLQAMNIPYTINPRIVRGLDYYTNTAFEIIYDGLGA
                     QNSLVGGGRYNGLIAQIGGKTIPAIGFAGGFERLLLALEEEKIELITPSKPDAYLVCV
                     GDNTRLHILPYLNALRTKGLIVDYDPDKSSLKAQLKSADTSGAKYAIIIGENELATKT
                     VNLKNLQTGEQIAIPMDEEYTINKMKN"
     gene            complement(6874..8226)
                     /locus_tag="PLW08_06035"
     CDS             complement(6874..8226)
                     /locus_tag="PLW08_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPL52197.1"
                     /translation="MIKPEIFRQYDIRGIVNEELNEESYYLIGKGFGTYLRNKGLKSI
                     VLGGDARHSTPLFMNAFSKGALETGCDIIDLGIVPTPVLYFSIWKLQSDGGAMVTASH
                     NPSQYNGCKLNLGLSSVYADELQKVLQIIQKGDFATGNGKLVKNNEIGNVYIDYIVNG
                     IKLQRPVKVIVDGGNGVGGPYLTTILRLLGCEVIDMFCEPDGDFPNHHPDPTIAKNME
                     ALSKAVVDNKYELGIGLDGDADRIGVIDEQGKLLLGDQILNILARDYLKHNPGKKIIA
                     DVKCSMNLFNDIKKYGGEPIMYKTGHANIKMFMKEMGVEFAGEMSGHIFLADRYLGFD
                     DAIYVSCRFVEIVSQTHNPVSTFLADQPKLYNTPELHTNCPDEKKFEVVAKVCEEFQK
                     EGYDVNDIDGARITFPDGWGLIRASNTTPVIVTRYEALTESRMNEIKNLIESKINKYL
                     "
     gene            complement(8241..8831)
                     /gene="rdgB"
                     /locus_tag="PLW08_06040"
     CDS             complement(8241..8831)
                     /gene="rdgB"
                     /locus_tag="PLW08_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPL52198.1"
                     /translation="MIRDILIATHNPDKLKELEELVNPLKLRLHFLRELPGFYPTEED
                     QETLQKNAMKKALEAAKYSGLITLADDTGLFIEALNGAPGIMSARFAGNSCSYSDNRQ
                     KVLRLLQDIQIRKAYFKTAVALSAPDGIIAVTEGRLEGEITTEERGNNGFGYDSIFAV
                     NGKTYAEMNADEKNHLSHRALAIKSIIPILQKIINN"
     gene            complement(8828..9355)
                     /locus_tag="PLW08_06045"
     CDS             complement(8828..9355)
                     /locus_tag="PLW08_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPL52199.1"
                     /translation="MKDDDVIEGREIPAEEQEKIKDKIIDDIDSTKLKFYENLRQKVR
                     GWTKEQLGSLGGKLGEYVFLLPDFFILVCRLALDKRVPVKQKMIMGGVVAYVMMPVDL
                     IPDFIPVIGLVDDLVLVVMALNMILNEIDPKILSDNWSGEGEVLEHLKKITAVAEQFL
                     DKHLLQKIKNVLKRL"
     gene            complement(9573..12296)
                     /gene="ppdK"
                     /locus_tag="PLW08_06050"
     CDS             complement(9573..12296)
                     /gene="ppdK"
                     /locus_tag="PLW08_06050"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPL52200.1"
                     /translation="MNTPKRVYLFGNGKAEGSAEMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTEVCTEYNKLGSAKLRKILSDDVHNAIRHIEDLVNMQFGSAENPLLVSVRSGA
                     RASMPGMMDTILNLGLNETTIQGLIKKTNNERFAWDSYRRFVQMYSDVVLGLKPESKE
                     EHDPFEVIIDTVKKEKGISSDLELTTEDLKNLVTIFKQTVEEKTGKKFPDDPWEQLWG
                     AIFAVFDSWNNERAKFYRKLNNIPDEWGTAVTVQAMVFGNMGFTSATGVAFTRDAATG
                     ENLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAKLAGISEEERLSKYPSLEEAMP
                     ETYKELFEIQKRLENHYRDMQDMEFTIQEGKLWLLQTRTGKRTGLAMIKIAMDMLREG
                     MIDEKTALKRIDPAKLDELLHPVFTKEGLAKAKVIANGLPASPGAASGQIVFFADEAE
                     AWAADKKKVILVREETSPEDLRGMAVAQGILTARGGMTSHAAVVARGMGKCCVAGAGA
                     LQIDYKARTMTVNGKVYKEGDWISLNGSTGQILEGKIDTQDPELSGDFGAVMQLADKY
                     TKMKVRTNADTPKDATVARNFGAQGIGLCRTEHMFFEGERIKAMREMILAEDETGRKK
                     ALAKLLPMQKTDFEGIYTAMNGFPVTIRLLDPPLHEFVPQEESAQKDIAEELGIPLEK
                     VKARVASLHEVNPMLGHRGCRLGNTYPEITEMQTRAIIEAALAVKARGISVLPEIMVP
                     LTSTAEEFEMQEDIIRKTAEKVFAEKQDRVDYLVGTMIEIPRAALTADKIAEHAEFFS
                     FGTNDLTQMTFGFSRDDAGVFLPVYLEKNLLKHDPFQIIDREGVGQLVKMGTERGRKT
                     RPDLKVGICGEHGGEPSSVEFCHLVGMNYVSCSPYRVPIARVAAAIAALND"
BASE COUNT         3803 a         2606 c         2442 g         4049 t
ORIGIN      
        1 ttgacggcaa ttccttaacc ttagaggcag tggaacaggt tgcttgctac aataccccca
       61 ttcgtctttc tgattccagt aagggaaaag tggctaaatg ccgtgcttat gtagataaaa
      121 taattgaaag cggcgaaatt gtttacgggc tgaataccgg tttcggcaaa ttcagcactg
      181 taaatattcc tgaagaaaac atagctgaac tacaattaaa tttaattcgc agtcattcaa
      241 ccaacatcgg agaaccctat tccgaagtgc aaacgcgggc tataatgctt ttaaggattg
      301 cagttttagc caaagggcat tccggaattc gtttagagac attggaaacc ctggtgcaaa
      361 tgttgaataa aggtattcag ccaataattc caatgcgggg ttcagttggc gcaagtggag
      421 atctttctcc gctttcacat ttggcattag tgctaattgg tgaaggtgaa gcaatttaca
      481 aaggtgagcg tcttccgggt gcttcagcaa tgcaaaaagc gggcataaac cctgtccaac
      541 ttgctgctaa agaaggactt gctcttaata atggcactca agttatgact gctttgggtg
      601 ttttaaacct tttggaagcg gagcgtttat gccgttatgc cgatatcatt gccgctatga
      661 gcatagatgc tttaaaaggg actcctaaag catttgatga attgattcat aatttaagac
      721 cacatccagg tcagaaaatt tctgcaaaaa atatcagaac ccttttggag ggaagtcctt
      781 tgcgggaatc tcatcgtaat tgcggcaatg ttcaagatgc ttacagtttg cgatgcacac
      841 ctcaagttaa tggtgcagtg agagatgcct gttcttatgt gcgaagaatt ttggaaatag
      901 aaataaattc agcaacggat aatccgctta tttttccgga tgagggtaag gttatttccg
      961 ggggcaattt tcatggtgaa cccttagcta tcgcattgga tactttaggt ttggcaatta
     1021 gtgaacttgc ctcaatttcc gaacgccgca ttgaacagat gttaaatcct gctctttcca
     1081 gaggtctttc tcctttttta gcaacgcgtc cgggaattga ttccggtttt atgattcagc
     1141 aaatgacggc agcatcgctg gtttcggaaa ataaagtgct ggctcatcct gcctgtgtag
     1201 attccattcc cacttctgcc aatcaggaag atcatatttc tatgggatca atttcggcta
     1261 taaaactttg ccaaatagtg caaaatgtgg cttccgttct gggaattgaa ttgctgattg
     1321 cctgttctgc actggatcaa aggcaaatat ctacttctcc tgttttggaa aaagtaaaat
     1381 cggctttaag agaaaatgtt gctcctttac aaggtgaccg ggttatgtat cctgatgtaa
     1441 ataaagcaat tgagttggtt cgttccaagc ggttggacga gatttgtcct gaaattggtt
     1501 gacaaaaatt tgccgtttag gggctattga actatgaaat tcagggatat aattatctgt
     1561 ctgctttgcc tttccggcat ttttgcctgc gaggtgaata ctttgttcaa tgcccgaaat
     1621 tattttaaat cggcacaagc aagaccttta accagtaatg gcagacctaa tgctcaagct
     1681 attgatgaat atacgaaagc gataaaaaaa tgcgggaaaa ttatttctac cgataaaaag
     1741 ggaaaaagag tagaagaagc atattatctg atggcaaaat ccctttatta taaaggcaat
     1801 agtgcttttc aggcaaaaga ccagtttcaa aaccttgtta tccgttttcc cgatagtaaa
     1861 tatgttcctg aggcatatat ttatattgcc aaaattttaa gagaaacgaa tcagcccaaa
     1921 gaagcggaaa aattgctgga tgaattcctc cgcaatccca aatatcgtaa acatcatcct
     1981 gaagcattat tcgttttagc cgactttgcg attaaggata aggactttat taaagctcaa
     2041 cattatctgg aaaggataat tacggaattt cccaaaacaa aagaataccg cgaagcatat
     2101 ttcctttttg gcaagaatta ttacgaacaa aaggattacg ataaatcgct ggaagcattt
     2161 aaaaaaatgc aaaaagcgcg cggtattgat aaaactataa agcttgaggg aacttattac
     2221 attggtttga acgaactgga acttggacag gcagaaaaag cactaaagac agctaagggc
     2281 ttgataaaat cggaatctcg tcccgataaa attccctttg tccgtttgct gaaggcaaga
     2341 gctcaatttg ctttgggcga tacaaccgag gcaagaactg aaattgaatt cattactaaa
     2401 aattatcctc gtactgaggg ttctgcaggt gcctattatt acctggcaga atactattat
     2461 tataatctgg gtgatattcc taaagcggtt ttgaactaca atcgggtacg cactgaattt
     2521 tcgggttctc cacttgctca aacagctcag gcaaaagcgg ttgccttagg atttgtagct
     2581 ccgcgcacag gtcttgactg tgaaacagga ctttctcaat ttttggatta tcattatcag
     2641 gcggcggaaa gttttttgaa tgtcctttct ttgcccgatt ctgctattgc ccgttacaaa
     2701 attgttattt ctcaaaaaga tactttaact gccagacgcg attctttaca gacggcaata
     2761 aacagaaata atgctattct ggattcgctt agagcgcttt taccgccacc ggaaattcct
     2821 gcaatagaca gtttaggact tttagaacag gcggaacttc cggtgacaca aacagatata
     2881 acatccgaat ctgcttttgc tgatacccta tctgaaacaa ttatcagcag tccagatagt
     2941 ttgcaaacct ttgcagatac attacaaata acggaatcgg aaattcctgt agcggatagt
     3001 ttatttgtga cagagacaat ttctgaaggt gatactttag cgactgaaga agaaaaaaat
     3061 aaagataacg gtcttccaga ttcctctttg gcagaagaaa aagttgaatt atcgtctccg
     3121 tttactgaaa caacagagcc agattcttta actttggcaa ttgctaaaca tattcaagac
     3181 ctggaaaagg aaaacgcaag atacagccag cgactggaaa aactggaaga aattattgag
     3241 cgttttgaaa gtgaaatagt tcctttctgt atgtttgcta ttggttccat ctatcataat
     3301 cgttctcctg aaagtcctga aaatagtaaa attatggcta aaatgcagga agaatatccg
     3361 gaaaataaat ttaccaaagc tctttatgct ctgcagaata atttgccggt acggttaatt
     3421 gaccccaaag aagaaaggga agaacagctt ttagatatgt attttgagca aatttacact
     3481 gctcctgatt ccgctcttgc cggtttacag aaacttacaa actctcccta ccataaaatt
     3541 aagctggctg ccaattaccg tttgggctgg tattacagtt ttgaacaaat tgatactctg
     3601 cttgcaaagc cctatttgaa agctgttttg gacgaacctg aagccggcga atatgctgtt
     3661 gttgcccgta gattctttga tggcaataaa ttccttctgc ggggttcatt tcctgttgtt
     3721 attcctcaaa ctgatactac tgcagttgat tccactggag ctgatataga agaaagcatt
     3781 aaggaatggt tttttcctgc tcctgcagaa atttatgaga ccctgccgga cagcttgaaa
     3841 atttatagtg gcattcctat gctgatggat tctttggtaa aagctgtttc tccgcctgaa
     3901 gacacgacgg aagagaaaaa catatctccc gaaatggaaa cactggatga taatattcct
     3961 ccaccggaaa aaaatcctgt tcctgcctta ccggaagagc tgattccgga aaaaaaagag
     4021 gaactgccac ttgaatgaag tggttgaatc atcccctttc gggacagcat ttctatctaa
     4081 aatcagctgt tttttttacc gggctggcta tcggactgga ttcttccctg cgtcagcttt
     4141 taatacagct ggtattgatt ttgactattc tgatttttga gccaaagtta tatctttctc
     4201 tgctcaatgc cttgaaaaaa ttgctgcctt tttttgccgg ttattgggtt tttgctactc
     4261 tttttggtca ggaatttctt actaccgtgt ttttcagtgt ccaaatttta tatctgatag
     4321 ctgttacggt ttatgtgttg ggaaatgtga aaatggaaca gatagcaagc caaagcagat
     4381 ggataagaaa atggaaaccg gtaaattctt tgttctatta tctctttgct acagttttct
     4441 ttaccaggtc tttttttgcc gaatacagaa agctgaaatt ggttaaagaa aacagttttt
     4501 ctctggctca acttttggat gttttagcaa atgtatctgc actctctcct caaattagca
     4561 attcagttac aaatatgcta aaatttggag gggaagaaaa aaccagcaat cctcaagcta
     4621 actatttggg tatcttcttc ttagccctat tagttatagt gcacggcttt taaaaaggtt
     4681 ggctatggca agatacttaa taaaaatatg ttatgatggc acgggatatt gcggatggca
     4741 aaaacagaaa aacggacttg ccattcagga agttatggaa aaaacgctgg agatatttgt
     4801 cggtaaaaaa acttccttaa ttgctgctgg aagaaccgat gcaggagttc attctttagg
     4861 ccaatatgct cattttgatt atagtggttc tatgcaggaa aagcaattgc ttttggcttt
     4921 taacagatat ttgcctgatt ctattaaagt gctggatata atgaaagtta gcccttgttt
     4981 aaatgctcgt taccaggctt atgaacgcag ttatcgctat cgtttggcta aagaaaaaac
     5041 accttttaac agaaattaca gcggtttctt gccccacttg catttaaacc tggagaaaat
     5101 gcaatctgct atacaatacc ttttagggaa acacgatttt tccacattcg ggaaaagcaa
     5161 tccggaagtt cccaatcgta tttgtgaact taaacatgta agtataagcg aaacggaaca
     5221 atattttgtt tttgatttta cagctgaccg ctttttgcat aatatggttc ggagaattgt
     5281 aggaactttg gctaatatat cacatttgca tttgcctcag gaaacgattt tggaaatatt
     5341 ggagaataga tgccctcgcc aaaatttagt gataactgct cctgcatcag gactttacct
     5401 gatggatgtt aaatatccgg caaagtttct tgacgaatct tacaatccta attttgagga
     5461 aaatgggatg gtaaataacc ctttgctaca ttcaataaaa taaaaggaag aggaaataaa
     5521 aatgaaatac aatatcccgc gcggaacttt tgatattctg cctgaagata gctataaatg
     5581 gcaattttta caggatagct ttcgtaaaat ggcaacttgt tttggttatg aagaaataac
     5641 tactcccatt tttgaggtag ctgaactctt tgaacgcagt tccggagaaa gttctgatgt
     5701 tgtccaaaaa gaaatgtatc gttttcagga taaaaaaggc agggaatttg ctttgcgtcc
     5761 tgaaggcact gctcctgtgg ttcgtagcta tgtagaaaat catctagata aactttcttc
     5821 tcgtacaaaa ttattttatt tgggacctat gtttcgttac gatcgtcctc aggcaggaag
     5881 ataccgtcaa ttttatcaat acggaataga atttatcggc agtaacaatc cttattacga
     5941 tgcagaagtg atagcagttg cttttttgtg gcttaataaa ttgggtttga ataacttgcg
     6001 tttggaaatt aatagtgtgg gctgtcctga ttgttctgct gtttatgata aggcattgct
     6061 ggaatattat agaccttttt tgcctaaact atgccctgat tgtgtgaaac ggttggaaac
     6121 aaatccccgt cgtttactgg attgcaaagt tccttcctgt atagaattta gaaggaatgc
     6181 cccttcgcaa atggattatc tggatgagtc ctgtaagaaa catttttctg cagtttgtga
     6241 ctacttgcaa gctatgaata ttccttatac tatcaatcca agaatagtgc gcggattgga
     6301 ttattataca aacactgctt ttgaaattat ctatgatggt ttgggcgcac aaaattccct
     6361 tgttggaggt ggaagataca atggtttgat tgcacaaatc ggagggaaaa ctattcctgc
     6421 tattggtttt gccggtggtt ttgaacgcct tcttctggct ttggaagaag agaaaataga
     6481 acttataaca ccttctaaac cggatgccta tcttgtttgc gttggagaca atacgcgtct
     6541 gcatattttg ccttatctga atgccctaag aactaaaggg cttattgtgg attacgatcc
     6601 cgataaaagc tctctgaaag cccaattaaa atccgccgat accagtggag caaaatatgc
     6661 tataatcatc ggggaaaatg aacttgccac taaaacagtg aaccttaaaa acctgcaaac
     6721 cggtgaacaa atagctattc cgatggacga ggaatacact atcaacaaaa tgaagaatta
     6781 aataggttgc cgaattccga ttcggtaaaa ggtttcaagg atgttttttt agcggtcttt
     6841 gacctcttta gcaaatcgga atttgccaac cccttacagg tatttgttga ttttggattc
     6901 tataaggttc tttatttcgt tcattctact ttcggttaat gcttcataac gggtaacaat
     6961 tacaggtgtg gtattggaag cacgaattaa accccagcca tcaggaaaag ttattctggc
     7021 accatcaata tcattaacat catagccctc tttttggaat tcttcacaga cctttgctac
     7081 cacctcaaac tttttctcat cgggacaatt ggtatgcagt tcaggagtat tataaagttt
     7141 tggctgatcc gctaaaaaag tgctgaccgg attatgagtt tgagaaacaa tttctacaaa
     7201 acggcaactg acataaatgg catcatcaaa acccaaatat ctatctgcca aaaagatatg
     7261 tccactcatt tcacctgcaa attccactcc catttctttc ataaacattt tgatattggc
     7321 atgacctgtt ttatacataa tcggttctcc gccatacttt ttgatgtcat taaacagatt
     7381 catactgcat tttacatcgg caataatctt tttcccgggg ttatgcttca aataatccct
     7441 ggctaagata ttcagtattt ggtcacctaa caatagttta ccttgttcat caataactcc
     7501 aattctatct gcatcgccat ccaaaccaat gccaagttca tatttattat caaccactgc
     7561 ctttgatagc gcttccatat tttttgctat tgtaggatcg ggatgatgat tgggaaaatc
     7621 accgtcgggc tcacaaaaca tatctatcac ctcacaaccc aaaagacgca aaatggtagt
     7681 taaataagga cccccaactc catttcctcc atctacaatc acttttaccg gacgctgaag
     7741 tttgatgcca ttaacgatat agtcaatgta aacattgcct atttcgttgt tttttaccaa
     7801 ctttccattg ccagtggcaa aatcaccctt ttgaatgatt tgtagtacct tttgcagttc
     7861 atcagcataa acactgctca agccaagatt taatttacaa ccattgtatt gagaaggatt
     7921 atgacttgcc gtaaccattg ctcctccatc actttgcagt ttccagatgg aaaaatacaa
     7981 aaccggagta ggaacaatgc ctaagtcaat aatatcacaa ccggtttcca aagcaccttt
     8041 gctaaaggca ttcataaaaa ggggagtaga atgtctggca tctccaccta agacaatact
     8101 tttcagaccc ttatttcgca agtaggttcc aaagcccttg ccaatcaggt aatatgattc
     8161 ttcgttcagt tcttcattta caataccacg aatgtcgtat tgacgaaaga tttccggttt
     8221 tatcattgat aactcctttt tcagttattg attatctttt gtaagatagg gataatagat
     8281 tttatggcta aagcacgatg ggaaaggtgg tttttttcat ccgcattcat ttcagcgtag
     8341 gttttaccat ttacagcaaa aatgctgtca taaccaaaac cgttattgcc acgctcttct
     8401 gttgttattt caccctctaa tctaccttct gtaacagcaa taatgccatc aggagctgat
     8461 aaagcaactg ctgttttaaa atatgctttc cgaatttgga tgtcttgtag aagccgtaac
     8521 accttttgcc tattatcaga atagctgcag gaatttcctg caaaacgagc tgacataatt
     8581 cctggtgccc catttaaggc ctcaataaaa agtcctgtat catctgccaa agtaattaga
     8641 ccgctatatt ttgctgcttc caatgctttt ttcattgcat ttttttgcaa ggtctcctga
     8701 tcttcctctg taggataaaa tccgggcagt tcacgcaaaa aatgcaaacg gagctttaag
     8761 ggatttacca attcctccaa ttctttcagc ttatcaggat tgtgagtggc aattaatata
     8821 tcccgaatca taagcgtttt agcacatttt tgattttctg cagtaggtgt ttatccaaaa
     8881 attgttctgc cacagcggta atctttttta agtgttccaa aacttcacct tcgccactcc
     8941 agttatcgga aagaattttg ggatcaattt cattcaaaat catattgagc gccatcacaa
     9001 caagcactaa atcatctacc agaccaatta cgggaataaa atccggaata agatcaaccg
     9061 gcatcataac ataagcaaca actccgccca taatcatttt ttgttttaca gggactcgtt
     9121 tatccagtgc caaacgacaa actaaaatga agaaatccgg taacaggaaa acatattcac
     9181 ccaattttcc acccagggaa cccaattgtt cttttgtcca acccctaact ttttgtctca
     9241 ggttctcata aaatttcagt ttggtggaat caatgtcgtc aattatttta tccttaattt
     9301 tttcctgttc ttccgcaggg atttctctgc cttcaataac atcgtcatct ttcataataa
     9361 acctccatct gcctataatt taatagtaat attgaagtca agcaaattat ttgaggtaga
     9421 gatgtcaaga agtcaaaaga tttgtgtagt cggagcttgc tgaagcttag cattttttca
     9481 agttcagata ttttgcggag gaacggtgtc ccctgactac aaaaaagcgg aggaacggtg
     9541 tcctccgctt atatataaat tgttttccag gattaatcgt ttaaagcagc tatagcagca
     9601 gcgacacgag caataggaac tctataagga gaacagctaa catagttcat tcccactaag
     9661 tggcaaaatt ctacactgct tggttctcca ccatgttctc cgcaaattcc aacttttaaa
     9721 tcaggtctgg ttttgcgtcc gcgttcagtt cccattttca ctaattggcc tacaccttct
     9781 ctgtctataa tttggaaggg gtcatgtttc agcagatttt tttccaaata cacgggtaaa
     9841 aacacgcctg catcgtcgcg ggaaaaacca aatgtcattt gagttaaatc atttgttcca
     9901 aaactgaaaa actcggcatg ttcagcaatc ttatcggcag ttaaggcagc gcggggaatt
     9961 tctatcattg ttcctactaa gtaatccacg cgatcctgct tttctgcaaa gaccttttct
    10021 gctgttttgc ggataatatc ttcctgcatt tcaaattctt cagcggtgct ggtaagcgga
    10081 accataattt caggtaatac actaattccg cgtgctttaa cagctaaagc ggcttcaatt
    10141 attgcacgag tttgcatttc tgtaatttct ggataggtat ttcccaaacg gcaacctcta
    10201 tgaccaagca taggatttac ttcatgtaac gaagcaacgc gtgctttaac tttttccaga
    10261 ggaattccca gttcttcggc aatatccttt tgagcggatt cttcctgagg aacaaattca
    10321 tgcaaggggg ggtctaaaag acggatagtt acaggaaatc cgttcatagc ggtataaatg
    10381 ccttcaaaat cagttttttg cataggtagt aattttgcca atgccttttt gcgtccagtt
    10441 tcatcttccg ctaaaatcat ttcccgcatt gccttaattc tttcgccttc aaaaaacata
    10501 tgttccgttc tacaaagtcc aattccctga gcaccaaaat ttctagcaac ggtagcatct
    10561 ttgggagtat ctgcattggt gcggactttc attttggtat atttatcggc aagttgcata
    10621 acagcgccaa aatcaccgct aagttcaggg tcttgggtat ctattttacc ttctaaaatt
    10681 tgtcctgtag agccatttaa ggaaatccaa tccccttctt tatagacctt tccattcact
    10741 gtcatagttc tggctttgta atcaatttgt aatgccccag ctcctgccac acaacatttt
    10801 cccattcctc tggcaacaac agctgcatga gaagtcatac ctccgcgagc agttagaatt
    10861 ccttgtgcaa ctgccattcc gcgtaaatcc tccggggagg tctcttctct aactaaaata
    10921 acttttttct tatcagcagc ccaggcttct gcttcgtctg caaaaaacac aatttgtccg
    10981 gaagctgctc caggagaagc aggcaaacca ttagcaatca cttttgcttt tgccaaacct
    11041 tcttttgtaa agacgggatg cagaagttca tccaatttag caggatcaat tcttttaaga
    11101 gcggtttttt catcaatcat accttcgcgg agcatatcca ttgcgatttt aatcatagca
    11161 agaccggttc ttttgccggt tcgggtttgt aagagccaca attttccttc ctgaatggta
    11221 aattccatat cttgcatatc gcgataatga ttttctaacc ttttctgaat ttcaaagagc
    11281 tctttatagg tctcgggcat tgcttcttcc agagaaggat atttacttaa gcgttcttct
    11341 tcactgatac cggcaagttt tgcccaacgc tgagaaccaa tttttgtgat ttgctgagga
    11401 gttctgattc cggcaacaac atcttcacct tgagcattga tcaaatactc tccattaaaa
    11461 aggttttcac cggtagcagc atcacgagta aaagcaacac cagtagcact ggtaaagccc
    11521 atattgccaa aaaccattgc ttgaacggtt actgcagttc cccattcatc gggaatgtta
    11581 ttcagtttac gatagaactt tgcgcgttca ttattccagc tatcaaaaac agcaaaaatt
    11641 gcaccccaga gctgttccca ggggtcatcc ggaaattttt taccggtttt ttcttccacc
    11701 gtctgcttga agatggttac cagatttttc aaatcttcag tggttagttc caaatcgctg
    11761 ctgatgcctt tttctttttt cacggtgtca ataataactt caaagggatc atgttcttct
    11821 ttgctttctg gtttaagacc taaaaccaca tcagaataca tttggacaaa acgacgataa
    11881 ctatcccagg caaacctttc attattggtt tttttgatta aaccctgaat agttgtttca
    11941 ttcaagccca aattcaaaat ggtatccatc attccaggca ttgaagcacg agcacctgaa
    12001 cgcactgaaa ctaaaagtgg attttcggca gagccaaatt gcatatttac aaggtcttct
    12061 atatgccgaa tggcattgtg cacatcgtca cttaaaattt tcctcaattt ggcagaaccc
    12121 aatttgttgt attcagtgca gacctctgta gtaattgtaa atccgggagg aaccggaacg
    12181 ccaataagat tcatctcggc aaggttggca ccctttccac cgagaagatt tttcatttct
    12241 gcgctacctt cggcttttcc atttccgaag aggtaaactc ttttaggtgt attcatttat
    12301 cctccataac tttaacatca ttaaagatat tttattgtct gtcacaatct tttttatggc
    12361 aaatctgtca atagctttct tgatttccaa ggtgtattac ggaagcattg cttacccaat
    12421 actaatgcca ttagcaatgg gtaagcaatg agtaagcaat gctattaagg aagaccctcc
    12481 taatacctaa tattattaaa gaccgttgaa taattggcat attaaagcaa tgtttagata
    12541 attccaagct catccccttt gtagtttgct aaagcgtatt taactaaata taaaaattat
    12601 attttatccc ataataacca ttggaccaag atgaaaaagt tccaacggaa catattttca
    12661 ctctgtcttc ttttcgtcgg gttgaaaccc ttctataata tctgtcgttc ctcctgaact
    12721 cttttcctca ataaatatag ttttgtaggt tcttttctta tcaccgggtt aaaacccgtt
    12781 gttaatatct gtcgttccta cggaactcat tctcgggaaa agagataagt tatttccttt
    12841 acacatttca ccgggttaaa acccgttgtt agtatctgtc gttcctacgg aactcattct
//
LOCUS       DAOOZV010000053        12368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5070_length_12368_cov_62.493706, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000053 DAOOZV010000000
VERSION     DAOOZV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5070_length_12368_cov_62.493706"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1066..1419
                     /gene="ssrA"
                     /locus_tag="PLW08_06055"
     tmRNA           1066..1419
                     /gene="ssrA"
                     /locus_tag="PLW08_06055"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1667..1936
                     /gene="rpsO"
                     /locus_tag="PLW08_06060"
     CDS             1667..1936
                     /gene="rpsO"
                     /locus_tag="PLW08_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPL52201.1"
                     /translation="MPLKPEAKQAIMAEFKLHESDTGSPEVQVALLTQRIKDITEHLK
                     EHPKDFSTRRGLLKLIGQRRRLLDYLKKKDITRYRNLIKTLGIRK"
     gene            2214..4445
                     /gene="pnp"
                     /locus_tag="PLW08_06065"
     CDS             2214..4445
                     /gene="pnp"
                     /locus_tag="PLW08_06065"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPL52202.1"
                     /translation="MHNFNITRRETDFCGRKLIVETGKMAKQANGSVFIQYGETAVLV
                     TATMSKEPAEDQDFFPLTVDYIEKMYAAGKIPGGFFKRESKPSTDATLMARVIDRSIR
                     PLFPEGFRNQVHIVVTVLSFDGVTDPSTMGVFGASLALSISDIPFNGPIASVTVGYID
                     NQFVVNPHFNEIETQSKLNLTVAGSESSIVMIESAALELSEEMMLDAVFTGHKEIQKL
                     CALQQDFISACGKEKVEVVLDSIPEEIMNKVENAFGAKIAEAAVIFGKQERQDAFDAA
                     EAEMVEMFSQQEDTDLSPWEETERYYKMAFDELIRRYVRDSILRKQHRVDGRGTDDIR
                     EITCEIDVLPRVHGSALFTRGETQSLGTLTLGGGTDEQVIDGLETEYKKSFYLHYNFP
                     PYSVGEVGRIGPTGRRELGHGNLAERALKAVLPDKDSFPYTIRIVADTLESNGSSSMA
                     SICSGCLALMAGGVPIKAPVAGIAMGLIMEGEDYVVLTDIMGLEDHLGDMDFKCAGTR
                     EGITAMQMDIKISGITREIMQIALNKAKSARFKILDIMAECIAEPRSDLAPWAPRIES
                     FKVPIDKIGEIIGPGGKMIKSIIEECQVQIDIQDDGTVRILSPDKESINKAKSIIKGI
                     AVDPVAGDMFEGQVTRIEPYGIFVKILGGTKEGMVHISEMYTGRINHPLDMVKLGDKV
                     QVKTLGMDKGKLSLSMKGVAGNPEPHPDAHREEQQNYHQQNRDDRNRGRNSGRNDYHR
                     HHR"
     gene            4467..5867
                     /gene="ahcY"
                     /locus_tag="PLW08_06070"
     CDS             4467..5867
                     /gene="ahcY"
                     /locus_tag="PLW08_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPL52203.1"
                     /translation="MDYKIADLSLADWGRKEIDLAEAEMPGLVALKERYRHRKPLAGA
                     KITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDNAAAAIAQRGIPVFAWKGET
                     LNDYWWCTLQALSWEEGPDLIVDDGGDATLMVHEGYRLEEQYKQTGKLPEVDAENAEM
                     RIVQELLINQLTIDPDKWHRAVKCLRGVSEETTTGVHRLYQRRNEGTLLFPAINVNDS
                     VTKSKFDNLYGCRESLADGIKRGTDIMVAGKIVMICGYGDVGKGCAQSMRGLGARVVI
                     SEIDPICALQAAMEGYEVNTVENMVEIADIFITATGNCDVIRVDHILKMKNNAILCNI
                     GHFDNEIQVNKLYEMEGVEKVNIKPQVDLIKLPNDKAIILLSEGRLVNLGNATGHPSF
                     VMSCSFSNQVLAQIELWQNEHEINVYTLPKKLDEEVARLHLPKLGAQLTQLSKKQAEY
                     INVPIEGPYKQDYYRY"
     gene            5870..6445
                     /locus_tag="PLW08_06075"
     CDS             5870..6445
                     /locus_tag="PLW08_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPL52204.1"
                     /translation="MATHKPVYVDELYGELALFTDDRKWVVVHTKPRCEKKLADYAKQ
                     HQITYYLPQYTSKRVYQRRKVDVNKVLFPSYVFVGIDFKGKQELLISGYTVNFLKVNA
                     QKELIDELQAIYYGRQKNVQMEPGLWLEKGLEVEIINGPLKGVRGVVEDQSKLTEVRL
                     QVNILKQAVLVKVRTQDIKIIGEFEIVEIDE"
     gene            6442..7374
                     /locus_tag="PLW08_06080"
     CDS             6442..7374
                     /locus_tag="PLW08_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPL52205.1"
                     /translation="MKILVTGGAGFIGSNVADAYLQAGHEVVIVDNLVTGNRRNINPK
                     AIFYEMDICDESLSEVFAKEKPDMVNHHSAQISVPLSIENPLLDVINNVYGWVNVLQN
                     CVRTGVKKVIYISSGGAIYGEAEEYPTSEKYNPKPLSIYAINKRVGEDYLYFYRHQYG
                     LNYTVLRYANVYGPRQISQGEAGVVSLFTEKLLKGEIPTVYRYDNEPDGMIRDYVYVG
                     DVVQANLLALERGEGEVFNIGTCIPTTTKDLYYAIAKQLGINREPYYGPARKGDLHRS
                     LLSCEKAKKVLGWKPETGLSEGISQVIKYLKELQ"
     gene            7371..8693
                     /locus_tag="PLW08_06085"
     CDS             7371..8693
                     /locus_tag="PLW08_06085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPL52206.1"
                     /translation="MKIAVIGSGYVGLTTSACFAEMGNTVISVDKDENKIEMLQAGKV
                     PIYEPGLEDMILRNMTAKRLNFTLDTKQAVQDSQIIFIAVGTPPGEDGSADLQYVIAA
                     AEEIASYLNEPKIIVNKSTVPVGTADLVNEKIQEVLNARGVDINYAVVSNPEFLKEGS
                     AIDDFMSPDRVVIGTDNPKAGEIMRTLYEPFCRTNDRILIMGIRSAEMSKYAANSFLA
                     TKISFINEISRLCDAYDADIAEVRNGMCSDSRIGYKFIFPGVGYGGSCFPKDIKALIN
                     MSRKVGYEPRILKAVEEVNSEQKKVLVEKVKAHFGKNLKGKTFAVWGLAFKPQTDDMR
                     EAPSVVIINELIAMGATVKAYDPVAMQEAKKVFGNNNSVTLCADEYETLKDAVAMLLI
                     TEWHQFRYPDFERMSKIMRQKVIFDGRNQYNPKAVKEMGFTYYGIGRR"
     gene            8810..9103
                     /gene="rpsP"
                     /locus_tag="PLW08_06090"
     CDS             8810..9103
                     /gene="rpsP"
                     /locus_tag="PLW08_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPL52207.1"
                     /translation="MVKLRLRRMGAINQPFYRIVAVDSRKKRDGKYIECIGWYDPKPD
                     PSKIKIDTERALYWLGVGAQPTDTVRSLLRKAGVLQIWHNNKQMKNQKTAEAE"
     gene            9130..9360
                     /locus_tag="PLW08_06095"
     CDS             9130..9360
                     /locus_tag="PLW08_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPL52208.1"
                     /translation="MKDLIEFIVKALVDDPSEVNITEITGDKITLYELRVSKADIGKV
                     IGKRGRTASAIRTIINAVSTKQGKRAELEIIE"
     gene            9361..9891
                     /locus_tag="PLW08_06100"
     CDS             9361..9891
                     /locus_tag="PLW08_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52209.1"
                     /translation="MKHPPLIGIGKLGRQNADGFHPVMIKPEYRSVFSELEDLYLIFN
                     SDRVFYVTISERMRKDNKTWIKLKEDGVSEEQFLHKDVVIAIDDTAIETEETSLNYLI
                     GYQVIFAGEELGIVEDFFFNGAQDVLQIVDSKGTEYLVPFVDYYIDTVIDNLRCVILQ
                     NAVDLVALYKVEAKKK"
     gene            9888..10559
                     /gene="trmD"
                     /locus_tag="PLW08_06105"
     CDS             9888..10559
                     /gene="trmD"
                     /locus_tag="PLW08_06105"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPL52210.1"
                     /translation="MIFEVLSLFPDVFSGFLNSSIIARAREKKLLEVNLTDCRQFSLN
                     KHHKVDDYPYGGFAGMVIQAQPIYSALIELLEKGNAPVVYFTPQGRPLTQKILESYSS
                     YKRIILLCGHYKEIDQRVRDLCVSDEISLGDYVLTGGEIPALAFIDGVSRLLPGVLSD
                     RESANSDSFSNEGLGFPCYTRPEIFMSLGVPEVLLSGNHKEIKKWALEQGKRLTVTRR
                     PDLKK"
     gene            10781..11134
                     /gene="rplS"
                     /locus_tag="PLW08_06110"
     CDS             10781..11134
                     /gene="rplS"
                     /locus_tag="PLW08_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPL52211.1"
                     /translation="MDILQQVSRDQIRTDLPDYRVGDTVKVHYKIKEGNKERIQVFQG
                     IVIQKRGAGISKTFTVRKVSSGVGVERIFPQNSPNIDKLEIIRHGQVRRAKLFYLRSA
                     QGKAGRIKEKRRFTV"
     gene            11475..11939
                     /locus_tag="PLW08_06115"
     CDS             11475..11939
                     /locus_tag="PLW08_06115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPL52212.1"
                     /translation="MLERVFYNSPDGFFHLLLEIEEETIMSINFCPAQVSSQPSNDLE
                     REIFRQFDLYFTGRSKEFDLPFFATGTVFQLMVWEEVMRIPYGKTLTYKEIAERIANP
                     NASRAVGKALNKNPVPILIPCHRVIGSKGKLTGYGAGIAIKKKLLELERSNL"
     gene            11939..12316
                     /locus_tag="PLW08_06120"
     CDS             11939..12316
                     /locus_tag="PLW08_06120"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPL52213.1"
                     /translation="MIIGIGCDIIEVERIQNAIEKNSRFCEKLYTASEIEYCSGKANS
                     YQSFAVRFAAKEAVMKALGTGWNGEVNWIDIEVVTSEKGSPTINLTGGAKDLADKLKV
                     NNICLSLAHEKGYALAFVILEGE"
BASE COUNT         3993 a         2055 c         2794 g         3526 t
ORIGIN      
        1 aaaaagaaat acagttttat aggttcttct cttatcaccg ggttgaaacc cgttgttaat
       61 atctgtcgtt cctacggaac tttcgtccaa aaagaaatac agttttatag gttcttctct
      121 tatcaccggg ttgaaacccg ttgataatat ctgtcgttcc tacggaactt cttgcaggaa
      181 aaagagataa tgactctcct gatagctttg cactgggttg aaacccgttg ttaatatctg
      241 tcgttcctac ggaacttttt tcccaaatat aaatatagat ttgtggtttt tttctcatca
      301 cttggttcaa aacccatcgt taatatctgt cgttcctacg gaacttcttg caggataaag
      361 agataatgac atttctgatt gcttttcgcc ggcttgaaat cccttgttaa aatctaaaaa
      421 aaatcccgtt agggatggca gatattaaca acgggttttg aacccggtga ctatgagaga
      481 aaccttacca ctataataaa aagttccgta ggaacgacag atactaatag cgggtttcaa
      541 cccgcacaaa gcaacaagga gaaaaagcaa aagatccagc aggtaaagat acaataaaat
      601 agataggact tatgtagaga aggaagtcat tagccagaaa aaatgaaaat ttataagaga
      661 aaatcataat tctgttattc gtacggaact ttctctcgga gaaagagata ataaccttcc
      721 tgattccttt tcgccgggtt gaaacccgtt gttaatatct gtcgttccta cggaactttc
      781 gtccaaaaag aaatacagtt ttataggttc ttctcttatc accgggttga aacccgttgt
      841 taatatctgt cgttcctacg gaactttttt ccccaataaa gatagttttg taggttcttt
      901 tcttatcaaa gggttaaaac ccttctataa tatctgctgg ggttgtctca caaactccaa
      961 aaattcaggt tgacagcaaa ggtattttga ttattatggt tatagtaaga gatttttgca
     1021 gggcaaatac atttatccaa ttatagttct tttatacagt ttataggggg tgaaatggtt
     1081 tcgacaggga atcagagtgc tcgaaatgca taccggagat tgttatacca ctccgttaac
     1141 aagtaacaaa ctgcaaatta actgcaaaca acaattacgc tttagcagca taagctgtta
     1201 acgttcaact tcttcggtcc gtcggaagga gattgggcgt cggaacagta cggatgcggt
     1261 agaactgatt gctgataagt tttacctaag tttaatcggc tgcggaaaag caggtctgat
     1321 tgtctttcac tgcttttctt aagataaaga caactaagta tgtagatttt cgggtgatcg
     1381 gttttctgga cgcgggttcg attcccgcca cctccaccaa aattctgcta atgaagaatg
     1441 aatttactca agagttaatt tagattgtta acaaagagaa caagaggaaa agaaaactaa
     1501 ttgatagttg atgaagtgat tttcagttta ttatactatg agacacttat aaataatcac
     1561 ttatgcctat gcatataacc aactttttaa cctcgctaca aaaaattaga gtggacaatt
     1621 atactaatct ctaaaatatg gttctcaaaa tcatctggag gatgaaatgc cgttaaaacc
     1681 tgaggcaaaa caagctatca tggcagagtt taaactccat gaatcagaca cgggctcgcc
     1741 ggaagttcag gttgctctgt taacacaaag aataaaagat ataacagagc acctgaaaga
     1801 acatcccaag gacttttcta cccggcgcgg tttgttgaaa ctgatcgggc aacgcaggcg
     1861 tctacttgat tatttgaaaa agaaagatat taccagatat cgtaatttaa tcaagacgct
     1921 cggcataagg aaataaactt tggtgtagct aattccggaa gaataacata ttgttgttta
     1981 atggttgacc ctgaggtcaa ccttccggat ttttgaaata gttatgagtc ctcactgcca
     2041 aataaatttg gtaaaagtta ttgaggcagt gatgactcct tacttaaagg attagccaga
     2101 agcaaccttc cagtaaaaaa ggcgggagta attagcaata agctaaaagg tttaggggaa
     2161 agaccttttc gcctattgct tcttctcccg ctaaagataa aaaaggagaa gttatgcata
     2221 acttcaatat tacccgtcgt gaaaccgatt tttgcggacg caaattgatt gtagaaacag
     2281 gcaaaatggc aaagcaagcc aatggcagtg ttttcattca atatggagaa acagctgttt
     2341 tggttacagc cacgatgtct aaagagcccg cggaagatca agatttcttt cccttaaccg
     2401 ttgattacat tgaaaagatg tatgcagccg gcaaaatacc cggcggtttt tttaaacgag
     2461 agtccaaacc ctcaactgat gccactttga tggcaagggt tattgaccgt tctattcgtc
     2521 ctttgtttcc agaaggtttt cggaatcagg ttcatattgt agtaaccgtg ctttcttttg
     2581 atggagtgac cgatccttcc acaatgggtg tttttggagc ttctttggct ctttccattt
     2641 ccgatatacc tttcaacgga cctattgcca gtgtgacggt tggttacatt gataaccaat
     2701 ttgtagtaaa tccccatttt aatgaaattg aaacccaatc caagctcaat ttaaccgttg
     2761 ccggaagtga gagttcaatt gtgatgattg aatcggcagc tttggaactg agcgaagaaa
     2821 tgatgctgga tgctgttttt acaggacata aggaaattca aaaactctgc gctttgcaac
     2881 aggattttat ttctgcctgc ggaaaagaaa aggtagaagt agttctggac agcattccgg
     2941 aagagataat gaacaaggta gaaaatgcct tcggtgcaaa aatagctgaa gcagccgtta
     3001 tttttggtaa acaggaaaga caggatgctt ttgacgcagc tgaagcagaa atggtagaaa
     3061 tgttttccca gcaagaagat acggatttaa gcccttggga agaaacggag agatactata
     3121 aaatggcttt tgatgagctt atccgtcgct atgtgcgtga ttccatttta agaaaacagc
     3181 atcgtgtaga tggtcgcgga acagatgaca ttcgggaaat tacttgtgaa attgatgttt
     3241 taccccgagt gcatggatcg gcacttttta ccagaggtga aacacaatcc ctggggactt
     3301 taactttagg cggtggaact gatgaacagg tgatagacgg cttggaaaca gaatataaga
     3361 agagctttta tctgcattac aattttcccc cttatagcgt tggtgaagtt ggaagaatag
     3421 gacctacggg tcgtcgtgaa ctgggacatg gaaatttagc ggaaagggct ttaaaagctg
     3481 tattgcccga taaagattcc tttccttaca ctattcgcat agtggcagat acactggaat
     3541 ctaacggttc ttcatctatg gcatctattt gtagtggatg tttagctttg atggcaggtg
     3601 gagttcctat aaaagctcct gttgccggaa tcgctatggg tttaataatg gaaggtgagg
     3661 actatgttgt tttaaccgat attatgggtt tggaagatca cttgggggat atggatttca
     3721 agtgtgccgg aacgcgggaa ggtataacag caatgcaaat ggatattaag atttcgggaa
     3781 ttaccagaga aattatgcaa attgctttaa ataaggcaaa atcagctcgc tttaagatat
     3841 tggatattat ggctgaatgc attgccgaac ccagaagtga tttagctcct tgggctcccc
     3901 gaattgaatc cttcaaagtg cctatagata agattggaga gattatagga cctggtggta
     3961 agatgataaa atcaattata gaagaatgtc aggtacagat tgatattcag gatgacggaa
     4021 cggtgagaat actttctccc gataaggaat ccattaataa agccaaaagt ataattaaag
     4081 gaattgctgt tgatcccgtt gccggtgata tgtttgaagg tcaggtaacc cgcattgaac
     4141 cctacggaat ttttgtgaaa attttagggg gaacgaaaga aggaatggtg catatttcgg
     4201 aaatgtatac cggacgtata aatcatcccc tggatatggt taaattagga gataaagtgc
     4261 aagtaaaaac cctgggaatg gataaaggca aactttcttt atctatgaaa ggagttgctg
     4321 gaaatcctga accacatcct gatgcccata gagaagaaca gcaaaattat catcagcaaa
     4381 atcgtgatga taggaataga ggtagaaata gcggtaggaa tgactatcat cgtcatcatc
     4441 gttaaaaaaa ataaatagag gaagaaatgg attacaaaat agccgatttg agtttagcgg
     4501 actggggcag aaaagaaatt gatttggcag aggcagaaat gcccggtctg gtagctttaa
     4561 aagaacgcta tcgtcatcgt aaacccctgg caggagccaa aataactggt agcttgcata
     4621 tgacaattca gaccgctgtt ttaattgaaa ctttggtaga actgggagct caagtgcgtt
     4681 gggcaagctg taatatcttt tctactcagg ataatgctgc agcagctatt gcccaaagag
     4741 gaattcccgt ttttgcctgg aaaggagaaa ccctgaatga ttactggtgg tgtacattac
     4801 aggcactttc ctgggaagaa ggacccgatt taattgtaga tgatggtggg gatgccactt
     4861 tgatggttca tgaaggatac cgtttggaag aacagtataa acaaacagga aaactgcccg
     4921 aagttgatgc cgagaatgca gaaatgagaa tagtgcagga attgcttatc aatcaattaa
     4981 ctatagaccc tgacaaatgg catagagcag tgaaatgctt acgcggagtt agtgaggaaa
     5041 caaccactgg agttcatcgc ctctatcaaa gaagaaatga aggcacattg ttatttcccg
     5101 ctatcaatgt aaacgattcg gttactaaat ctaagtttga taatctttac ggctgtagag
     5161 aatcccttgc ggatggcata aaaagaggaa cggatattat ggttgctggc aaaattgtta
     5221 tgatttgcgg ttatggagat gtgggaaagg gctgtgcaca atcaatgcgt ggtttgggtg
     5281 ccagagttgt aatcagtgaa atagacccta tttgtgcatt acaggcagcg atggaaggtt
     5341 acgaagtgaa tactgtagaa aatatggtag aaattgccga tatttttatt acggcaacag
     5401 gaaattgtga tgttattcgg gttgaccata ttttgaagat gaaaaacaac gctattttgt
     5461 gtaacatagg ccattttgac aatgaaattc aggtaaataa actctatgag atggaaggtg
     5521 tggaaaaagt gaatattaag ccacaagttg acttaatcaa acttcccaat gataaagcaa
     5581 ttattttgct ttcggaaggt cggctggtaa atttgggtaa tgctacaggt catccttcct
     5641 ttgtgatgag ctgttctttc agtaatcagg ttttggcaca aatagaactc tggcagaatg
     5701 aacacgagat aaatgtttac actttgccta agaaattgga tgaagaagta gcacgcttac
     5761 atcttcccaa gctgggagca caattaactc agctctccaa aaaacaggca gaatatatta
     5821 atgtgcctat tgaaggaccc tataaacagg attattatcg ctattaaaga tggctacgca
     5881 taaacctgtc tatgtagatg aactttacgg tgaacttgcg cttttcacag atgacaggaa
     5941 atgggttgta gtgcatacca aaccacgctg tgaaaagaaa cttgccgatt atgcaaaaca
     6001 gcatcagata acttattacc tgccacaata tacatccaag cgggtttatc aaagacgcaa
     6061 agtagatgtg aataaagtgc tttttccctc ttatgtattt gtagggattg attttaaggg
     6121 taagcaggaa ttattgattt cgggttatac cgtcaatttt ctgaaggtga atgctcaaaa
     6181 agaactgatt gatgaattac aggcaatcta ttatggaagg cagaaaaatg tgcaaatgga
     6241 accaggactt tggctggaaa agggtttgga agtggaaatt ataaacggtc ccttgaaagg
     6301 tgtcagaggc gtagttgaag accaatccaa gctcacggaa gtgcgtttgc aggtaaatat
     6361 tttaaagcag gcagtgctgg ttaaagttag aacacaagac atcaagatta ttggtgaatt
     6421 tgaaattgtg gagattgacg aatgaagatt ttagtaacag gcggagcagg ttttatcggt
     6481 tccaatgtag cagatgccta tcttcaagca gggcacgaag ttgttattgt agataattta
     6541 gtaactggca acaggcgtaa catcaatcct aaagcgattt tttacgaaat ggatatctgt
     6601 gatgaaagtt taagcgaggt ctttgctaaa gaaaaacccg atatggtaaa tcatcattcg
     6661 gctcaaataa gcgttccgct ctccattgag aatccccttc tggatgtaat aaacaatgtt
     6721 tatggctggg tgaatgtgtt gcaaaattgc gttaggacag gggttaaaaa agttatctat
     6781 atttcttcag gtggagctat ttatggtgag gcggaagagt atcctacatc cgaaaaatac
     6841 aatcccaagc ccctttctat ttatgctata aataaaagag taggagagga ctacctctat
     6901 ttttaccgtc atcaatatgg cttgaattac acggttttgc gttatgccaa tgtttacgga
     6961 ccaagacaaa tatcccaggg agaggcagga gtcgtctctc tttttacgga aaaactgctg
     7021 aaaggtgaaa taccaactgt ctatcgttat gataatgagc cggatggaat gatccgggac
     7081 tatgtttatg tgggggatgt tgttcaagcc aatttactgg ctctggaaag gggagagggt
     7141 gaagttttca atattggaac ttgcattcca accactacta aagatttata ttacgcaata
     7201 gcaaaacaat tgggaataaa ccgtgaaccc tattatggac ctgccagaaa gggagattta
     7261 catcgttccc tgctttcctg tgaaaaggca aaaaaggttt tgggctggaa accggaaaca
     7321 ggtcttagcg aaggcatttc ccaagttata aaatatttaa aggagctaca atgaaaatag
     7381 cagttatagg aagtggctat gtgggcttaa ctacaagtgc ctgttttgcg gaaatgggaa
     7441 atacggtaat cagtgtagat aaggatgaaa acaaaataga aatgctgcaa gcgggcaaag
     7501 ttcctattta cgagccgggt ttggaagata tgattctgcg caatatgaca gccaagcgtc
     7561 ttaattttac attagataca aaacaggcag tgcaggattc acaaattatt tttatagctg
     7621 taggaactcc tcccggagaa gacggttctg ctgatttaca atatgttatt gctgctgcag
     7681 aagaaattgc ttcctacctg aatgaaccca aaattatcgt taataaatcc acagttccgg
     7741 ttggaacggc agatttggta aatgaaaaaa ttcaggaagt gcttaatgcg cgaggtgtgg
     7801 atataaatta tgctgttgtg tccaatccgg aattcctgaa agagggctct gccatagatg
     7861 attttatgag tccggataga gttgtgattg gaacggataa tcctaaagct ggagaaatta
     7921 tgagaaccct ctatgaaccc ttctgcagaa caaatgaccg cattttgata atgggcatcc
     7981 gttcagcaga aatgtcaaag tatgcggcaa attcttttct ggctacaaaa atatctttca
     8041 tcaacgaaat ctcccgtctt tgcgatgcct acgatgcaga tattgcagaa gtgagaaatg
     8101 gaatgtgcag tgattcccgc attggctata aatttatttt tcctggagtg ggttatgggg
     8161 gaagttgttt tcctaaggac atcaaagcac ttatcaatat gtctcgtaaa gttggttatg
     8221 aaccgcgtat tttaaaagcg gtggaagaag ttaactccga acagaagaag gtcttggtgg
     8281 aaaaagtgaa agctcatttt ggcaaaaact tgaaaggtaa gacctttgcc gtttggggct
     8341 tggctttcaa accgcaaacg gatgatatgc gtgaagcgcc ttccgttgta ataatcaatg
     8401 aacttattgc tatgggagct acggttaaag cttatgatcc tgtagctatg caggaagcga
     8461 aaaaggtttt cggcaataat aattccgtaa ctctttgtgc cgatgaatat gaaaccctga
     8521 aagatgcagt ggcaatgtta ctgataacgg aatggcatca gtttcgctat cccgattttg
     8581 aaaggatgag taaaataatg cggcagaaag tgatttttga cggtcggaat caatataatc
     8641 ctaaagcggt taaggaaatg ggttttacct attatggaat tggtcgtcgc taaaacagtg
     8701 caaagctttt tcagtaaagg cataaaaaat ttatcttgac ggaaataagc attctaaaat
     8761 ctaagaataa aattgaaaat aattatgttc ataatgacaa ggagaatgaa tggtaaaatt
     8821 aagattgaga aggatgggtg ctataaatca gcccttttac cgaattgtag cagttgattc
     8881 acgcaaaaag cgtgacggca aatatattga atgcattggc tggtatgatc ccaagcccga
     8941 tccttccaaa ataaaaatag atacagaacg tgctctttat tggcttggcg ttggtgcaca
     9001 gccaacagat acagttcgtt ctctgttacg caaagcaggt gtcttgcaaa tttggcataa
     9061 taataaacaa atgaagaacc agaaaactgc ggaagcagaa taaagtataa aagaaaactg
     9121 aggtgaaaaa tgaaagatct cattgaattc attgttaaag ctctggttga tgatccttcg
     9181 gaagtgaata ttaccgagat cactggcgac aaaattaccc tttatgagct tagggtttcc
     9241 aaagcagaca ttggaaaagt aatcggcaaa cgcggtagaa ccgcaagtgc catcagaacc
     9301 attattaatg ctgttagcac aaaacagggg aagcgtgccg agctcgaaat tatcgagtaa
     9361 atgaaacatc cccctcttat cggaatcggc aaactgggta ggcagaatgc agatggcttt
     9421 catcctgtga tgatcaagcc ggaatacaga agcgtgtttt ctgagcttga ggatctgtat
     9481 ttgatcttta acagcgatag agtgttttat gtaactatca gtgagagaat gcggaaagat
     9541 aataaaacct ggataaaact gaaggaagat ggtgtttccg aagaacaatt tctacacaaa
     9601 gatgtagtga ttgccataga tgacacggca atagagacgg aagaaacatc tcttaattat
     9661 cttataggtt accaggttat atttgcgggt gaggaattgg gaattgtaga ggactttttt
     9721 ttcaacggag cgcaggatgt tttgcagatt gtggatagca agggaacgga atatctggtt
     9781 ccttttgtgg attattatat agatacggta attgataatc tgcgttgcgt gattctgcaa
     9841 aatgctgtag accttgttgc tctttataaa gtagaagcaa aaaagaaatg atctttgaag
     9901 tcctctccct ttttcccgat gtctttagcg ggtttttaaa cagcagcatt attgcccgag
     9961 ccagagagaa aaaactgctg gaagtaaatt tgacggattg tcgtcagttt tctctaaata
    10021 aacatcataa agttgatgat tatccttatg gtggttttgc cggaatggtt attcaggcac
    10081 aaccgattta tagtgcttta atagaacttt tagagaaagg aaatgctccg gttgtttatt
    10141 tcactccgca aggtagacct ttaactcaaa agattttaga gagttactct tcctataaac
    10201 gcataatcct gctttgcggt cattataagg aaattgatca aagagtgcgt gatctctgtg
    10261 tttcagatga aatatcttta ggggattatg ttttgaccgg aggcgaaatt ccggctctgg
    10321 cttttattga tggagtaagt agattactgc caggagtgct gagtgatagg gaaagtgcca
    10381 atagtgattc ttttagtaat gaagggcttg gttttccctg ttatacaaga ccggaaattt
    10441 ttatgagctt aggcgttccg gaagttttgc ttagcggaaa tcataaagaa ataaagaaat
    10501 gggcattgga acagggaaaa cgcttaacag taacccgcag accagatttg aaaaagtaaa
    10561 caaacaagca ggaagaaaga aacctaaaac aactaatgtg agcaaggaat ttgccggtag
    10621 ggaaaattta ttaatttccg gctttatttt atgacagaaa tgctcttaat gaagtaaata
    10681 aatattagaa cgggatagag aaaactcctt ttttaatccg tttaatcctt gtttgcacac
    10741 ggttttttac cttccgagta tatatatggg aggaacccaa atggatattc tgcaacaagt
    10801 tagcagagac caaattagaa ctgacttacc ggattatcgc gttggtgata ccgttaaagt
    10861 tcattataaa attaaagaag gtaacaaaga acgcattcag gtttttcagg gtatcgttat
    10921 tcaaaaacga ggcgcaggta tttctaaaac atttaccgtg cgtaaggtct ccagtggtgt
    10981 tggagtggag aggatttttc ctcaaaattc tccaaatatt gataaacttg agattattcg
    11041 tcatggacaa gtaagaagag cgaaactatt ttatctgcgt agcgctcaag gtaaagcagg
    11101 aagaatcaaa gaaaaaagaa gattcactgt atagttacac agaaaaactc aatcttaagc
    11161 cccttcttaa attttaggaa ggggctttgt tttgtgtagc cggagtacgc cgtccctcca
    11221 ctttttaagg aagaatgaaa ataaattgag gcgaaaatga taataagaaa agtgaggagt
    11281 cattatcgct gaaataaatt atactttacc tacaaactca tttagcgatg aggactcgta
    11341 acgataaagt taataaatct gtattatcta tttgtgaggg ataataagtc gtcacgattc
    11401 ataagcgctt tcagagaaat ttataaggaa aaaatattaa ggcgctaatg actcctgact
    11461 taaaggagtt ttatatgtta gaaagagtat tttacaatag tccggatggc tttttccatc
    11521 tgctgctgga aatagaggaa gagaccatta tgagcatcaa tttttgtccg gctcaagttt
    11581 ccagccaacc ttccaatgac ctggaaagag aaatattccg ccaatttgac ctttatttta
    11641 caggaaggag caaagagttt gaccttcctt tttttgcaac cggcactgtt tttcagctta
    11701 tggtttggga agaagtgatg cggatacctt atggtaaaac attaacttat aaggaaatag
    11761 cagagcgcat tgctaatccg aatgccagca gagctgtggg caaagcactg aataaaaatc
    11821 cggttcctat tcttatacct tgtcacagag taatcggttc caaaggaaaa ttaacaggtt
    11881 acggagccgg tatcgctatc aagaaaaagt tgctggaact ggaaaggagc aatctgtaat
    11941 gattattggt attggttgtg atattattga agtagaacgt attcaaaatg ctatagagaa
    12001 aaattcccgt ttttgtgaaa agctttatac tgcttcggaa attgagtatt gttccggcaa
    12061 agcaaattcc tatcagtcct ttgcagttcg ttttgctgcc aaggaagcag tgatgaaggc
    12121 attaggaacc ggttggaatg gagaagttaa ctggatagat atagaggttg taacttcaga
    12181 aaagggttct ccgactatca atctaacagg aggtgcaaaa gaccttgcag ataagctaaa
    12241 ggtaaataat atttgtctta gtttggctca tgaaaaggga tatgcgttag cttttgttat
    12301 tttggaagga gaataaaagt ttagttgagg catagagaat aatccacaaa accatacttc
    12361 tttgggaa
//
LOCUS       DAOOZV010000054        11919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5312_length_11919_cov_59.616403, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000054 DAOOZV010000000
VERSION     DAOOZV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_11919_cov_59.616403"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1059
                     /locus_tag="PLW08_06125"
     CDS             223..1059
                     /locus_tag="PLW08_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL52214.1"
                     /translation="MNGIVFFKTRELGLIRNFYTSLLALPVWLEQDSCVIFKAGNQLL
                     GFCAGESSELCGTITFFFDGKEQVDSAYKKLKSIAKTAPQENPAFKIYHFWATDPEGR
                     TLEFQCFEQQLEPYLTLDEGLKTRRSIRQYTSEPVSDELLNKVFELCRYSPTACNLQG
                     YYYVVIKNREILEKIVALRGPSGKPILASPFAIAVASQGDISHRKIQDACIAAYHLLL
                     SAKAYGLGTCWVTDMDKDEIKDLLGIPHQDYIACLTPIGYPAEHFPVPERHQVSDFVR
                     YL"
     gene            1056..2009
                     /locus_tag="PLW08_06130"
     CDS             1056..2009
                     /locus_tag="PLW08_06130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPL52215.1"
                     /translation="MKPILFLTRRIPEPAIKRLEEKFTLKINPYNRALTHQELIEGVK
                     DAEALICLLTDNIDKEVISAAPKLKVISSYAVGYNNIEVEYATQLGIAVCNTPGVLTE
                     TTADLTWALILATCRRISESERFLRKGNFKGWEPMLMLGLDVYGKTLGIIGMGRIGQA
                     VAKRATGFAMRIIYYNDVPVSNTLPFETTETDLQTLLKEADIITLHLPLTKETFHLIG
                     KEEFALMKENAVLINTSRGAVIDEKELIKALSEKRIFSAGLDVYENEPDIPQELLALE
                     NVVLLPHIGSASIETRTKMALLAAENAIAVMEGRKPPAQVN"
     gene            2187..3290
                     /locus_tag="PLW08_06135"
     CDS             2187..3290
                     /locus_tag="PLW08_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL52216.1"
                     /translation="MQKTNIGIFSGLSEEVVEELTKDLERIKFKKGEAIITEHSLSDN
                     LFFISKGKVEIDKDLSNPDIPFTQLSTLGAGDFFGEMGIIEDSTRSASVVATEDVELM
                     VIPREAFKEMLFAYPIIMLNLTKVISSRLRNTNERFVELMDEMLKKNRLMAIGLAASK
                     IIHDIKTPLTVIVLTAQLLENIFPESAEFTDNIVKQTKLIDQLVHEILDFARGTESPP
                     LIQKVNLDSFLQEISELYSASLKERKINFVVENKVNDCVYFDEGKIRRVLINLIKNAL
                     EAVSEAGEIKIISSVSSGWLQISVIDNGPGVSQKVKEELFQPFVTDGKTQGTGLGLAI
                     CKKLVQEHHGRLEYIPVEPHGSRFDIRIPQNTK"
     gene            3357..3548
                     /gene="rpmB"
                     /locus_tag="PLW08_06140"
     CDS             3357..3548
                     /gene="rpmB"
                     /locus_tag="PLW08_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPL52217.1"
                     /translation="MSKVCDICGKTAQVGNHRSHALNATKRRFYPNLHEIRVAGDGGT
                     KKIKVCSSCLKANKVRKAV"
     gene            3696..4682
                     /locus_tag="PLW08_06145"
     CDS             3696..4682
                     /locus_tag="PLW08_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPL52218.1"
                     /translation="MLIEAIQNEVEKSSAVLGNVRSEIARVIVGQQEIIDRLLIGIIA
                     NGHILIEGVPGLAKTLIISSLASVFDASYSRIQFTPDLLPADITGTMIYNPKSGDFSV
                     KKGPVFANFILADEINRAPSKVQSALLEAMQERQVTLGDNTFPLPNPFFVMATQNPIE
                     QEGTYPLPEAQIDRFFMKLKIKYPAYDEERQILERMVNEQPIPLQKVLSPKDIEEMRE
                     VMHKVYMEEKLKDYILHLVNSTRHPERYPRLNDLKGLIEFGASPRATIFLARAAKTNA
                     YISGRAYVIPDDIKMIAKDVLRHRIILSYEAEAEELTSEDIINRILDEIEVP"
     gene            4696..5580
                     /locus_tag="PLW08_06150"
     CDS             4696..5580
                     /locus_tag="PLW08_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPL52219.1"
                     /translation="MFTQSVSDILKKIRRIEIRTRNPVAEIFRGEYHSRFKGQGLEFS
                     EVREYQPGDNYRDIDWNVSARLGQLYIKKYRETRELRVVFLVDVSASQEFGTRSMIKR
                     ERIAELVAALAFSAVANQDLAGLILFSDRVEKFLPPRKGRNNALAILREVLYYEPKSK
                     KTSLQVACEYAHKMLKKRCILFIISDWIDSGYEHSLKILATKNDVIAIRVLDDSELEL
                     PRAGILSLQDPETGEEVWINSSDSKLRKAYQEIVNKEQKDLEEFFRKCNCDYLLIRTS
                     ESYINSLRKFFTLRERRR"
     gene            5577..6467
                     /locus_tag="PLW08_06155"
     CDS             5577..6467
                     /locus_tag="PLW08_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52220.1"
                     /translation="MKRIKVIVSFLFLLLPLFIRAELSQELQGVDSLKVGTPFLFKIT
                     TDFAIKGVSIPDTLEDFAILESKRDEKNPRCWIIKIVPLNTGALSFPRIEIQPVDSSV
                     TPVYTDGFRIYVLSVLAEGDTLLRDIKPLKKYSLQPPFWVYLLLILLALAGGIYLWLK
                     RPEKPKREYIAQPVIREPVLPAWEKALKALDELIAENLPAKGETVLYHFRLSNILRAF
                     LEETYRFPALEMTGREIAVSLNRRNIPKAQEIRKFINYCDLVKFAKREPSAGEIEENT
                     LWLRNYLLSFSEQTRETENA"
     gene            6460..7461
                     /locus_tag="PLW08_06160"
     CDS             6460..7461
                     /locus_tag="PLW08_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPL52221.1"
                     /translation="MLKFGHPWLLLLLLVIPLYLIWEFVFQKKRRLYLPHSRVKQLRY
                     LSHSQYPYHFLYPVLRSCILLFLCIAVAQPRWGIKTRDLSNKGVDIVMAIDISGSMLA
                     MDFAPKNRLSAAVSVAKDFVKRRPNDRFGLVAFSEYALTQVPLTFDHLAMLNSLDKLK
                     VNEEASATAIGMGLAKAVARLKNSTAKSKVIILITDGVSNTGEIDPLTAAEMAKELGI
                     KVYPIGVGSKGLVPFPYSDPIFGTRYINTYIDLDMETLDKIAATTGTGKAALATDAKG
                     LADIMNEIDRLEKTLFTTQFRYNYSEQFMPFLWLAFAFLVLELLLKIFILPVLPEQL"
     gene            7458..8453
                     /locus_tag="PLW08_06165"
     CDS             7458..8453
                     /locus_tag="PLW08_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPL52222.1"
                     /translation="MNIANPSYLALLALCLVFLLLLFGRERKLKRRFAQYAELHLREH
                     YYHSHSPFWTGFKLFLSILALGCIIIALARPQWDYENKELQSSGMDIIFAIDVSKSMD
                     ATDMMPSRLLRAILQIGSFLEQVKNDRIGIIAFAGTATLQCPLTDDYEAVRIVLNGLN
                     SNTVEISGTDIGSALRLAENAFPEGSKSKTLVLISDGEDLRHSALREARILKTKGIRV
                     YTMGVGSPEGTIIRHPETGEEVKSKLDEATLQEIARITDGEYYRVTPGGEEIQLILKR
                     IYESESTRRGKKVTILKEQYYLFGILAIILLMIESFIDPRKRKRGIMAAEKSNEH"
     gene            8443..9027
                     /locus_tag="PLW08_06170"
     CDS             8443..9027
                     /locus_tag="PLW08_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL52223.1"
                     /translation="MSTKTRRNIIIIIAVILALLILELIFRPKVVRQAFAEPFYRAKK
                     YPQAENLFRKNSTKNDAPANANLAKCLYRQNRFAEADSAATIALNSAKDKKDILYDRG
                     NIAYKQQDYSKALRDYKEALLLNPEDDDLRANYELTLRKMNKQPPPAVGLQQNKDDKK
                     KEAIRNILEGLDNKESTDRQQLRSKQSLKTDKWW"
     gene            9030..10811
                     /locus_tag="PLW08_06175"
     CDS             9030..10811
                     /locus_tag="PLW08_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPL52224.1"
                     /translation="MKKALLLVLLFPLVLSALKVDLSVNKNTINLNEQLELILKISDD
                     NRIKVQEPTPPSIPLFSFRNMTSSSSSSVVLEGTKLISEFSETYRFIYFPLKTGTTTI
                     PSFQVKVNGKYYATRPISIEVVKAEQKTPGQSSPPVPGIIPFGFDDSFFGGDREGWAG
                     DTFILALPKVQKVYRGFPAIVSYYLYTDEMVGSFNLENEIDSDGYGKSTYEQPTMLNY
                     EEVTINGKQYKRALIKRLALIPNREGRLQAPILEGYARLYGFGLLNKNLQSEGGIIDV
                     LPLPTNNVPPSFGGAVGNFKLSHSLSRKELSLGETITFTLKIEGRGNFNQFSAPQFAS
                     GKGFQVSTPMVIDNLNAGIEGTRTYYYTLIPQDKGVYELPELNFAWFDNDSGSYKIYR
                     SPKEQIKVSSANVLSYFSRLRQSRNPKAMLPKISRESYPDYVPYVKQPWYWILVVLIV
                     LATIVVSFVAVERKMKRDNPERYASLKAEKVLLHYLKPATAAAKNLSLDFYPLAEKAL
                     FQYLASKYKLSNHLSTQEKLQALSEKNIPEWLLDEIQIFLSHSQAARFMPETDRAVNL
                     EEDLARIRKIFTGFSRLSKNRKNRSGK"
     gene            10808..11662
                     /locus_tag="PLW08_06180"
     CDS             10808..11662
                     /locus_tag="PLW08_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52225.1"
                     /translation="MKKCRQRAPFLSHNKRSLNLQSCNELQLHRICSYSKLPLLLVFV
                     IILFCPTLFAQADSLQTTNFSGSGELTPLPDTLDKAIRLAEQKKNPDLFYNLGVEFFS
                     RGEIGMANLYFLKALNINSAHSPARTNLELSIHYSPDADLYPEQLFLVQVLFKVLNYL
                     SVNRLAILGLVFLFLSALSLCWLLFYNPEKERALPILTLTFCLLLCVSAFVTLGIKLH
                     HQQHNRSAVVIVRETELLSPANNNVIQTVHSGLIVKIVQDNGNYYVVRLPNNQLGRLN
                     KEAVRKVI"
BASE COUNT         3645 a         2146 c         2547 g         3581 t
ORIGIN      
        1 tcccttgctt caaacccaac atcaacccaa cctttaggat gggtagatgc agagtggagg
       61 atggggtaga tgatgggttg taagtaagta agtaagttcc tattgctatg cagggttaaa
      121 cttgtagcta taaactcaaa taatggtgtc caaatcaatc ggatttgcct gttaacctga
      181 aacagattaa aatatattat aaataggaaa aaaggagata tgatgaacgg aatagtattc
      241 ttcaaaacaa gggaattggg tctgatacgg aatttttaca cttctctgct ggcacttcct
      301 gtctggttag aacaggattc ctgtgtaatt tttaaagccg gtaatcaatt gcttggtttc
      361 tgcgcaggcg aaagtagtga actttgcggt acgattacct tcttttttga cggtaaagaa
      421 caagttgata gcgcttataa aaagctgaaa agcatagcga aaacagcgcc gcaggaaaat
      481 ccagcattta aaatttatca tttttgggca actgatcctg aaggcaggac tttggaattt
      541 cagtgttttg aacagcaatt ggagccctat cttactttgg atgagggctt aaaaacccgc
      601 cgcagtattc gtcaatacac ttcggaaccg gtttcggatg agcttttaaa caaggttttt
      661 gaactttgtc gctattctcc aaccgcctgc aatctgcaag gttattatta tgtagtgatt
      721 aaaaataggg aaattttaga aaagattgtt gccctgcgtg gcccttccgg taagccgatt
      781 ttagcttctc cttttgctat tgccgttgcc tctcaaggtg atatctctca tcggaaaatt
      841 caggatgcct gtattgctgc ttaccattta ttgctttccg caaaagctta tggcttggga
      901 acttgctggg ttaccgatat ggataaagat gaaataaaag accttcttgg cattccccat
      961 caggattata ttgcctgctt aactccgatt ggctacccgg cagaacattt tcctgttccg
     1021 gaaaggcacc aggttagtga ttttgtgagg tatctatgaa accgatatta tttttaaccc
     1081 gccgtattcc agaacccgca ataaagcgtt tggaggaaaa atttaccctg aaaatcaatc
     1141 cttataaccg cgctttaact catcaggaac tgattgaagg agtgaaagat gccgaggcac
     1201 ttatttgttt attaaccgat aatatagata aagaagttat ctccgcggct ccaaaattga
     1261 aagttatttc ctcttacgca gttggctaca acaatattga agtggaatat gccactcaac
     1321 ttgggattgc tgtctgtaat acaccgggcg ttttaacaga aaccactgcc gatcttactt
     1381 gggctcttat tttggcaacc tgcagaagaa tttcggaaag tgagcgtttc ttgcgcaagg
     1441 gaaactttaa aggttgggaa ccaatgctga tgttaggact tgatgtttac ggcaaaacac
     1501 ttggtataat aggaatggga agaattggtc aggcagttgc caaacgcgct actggttttg
     1561 caatgcggat tatctattat aatgatgttc ccgtttccaa tactttacct tttgaaacaa
     1621 cggaaacgga tttgcaaaca ctgctgaaag aagcggatat cattaccctg catttgccct
     1681 taacaaaaga gacatttcac cttataggca aagaggaatt tgccttaatg aaagaaaatg
     1741 ccgtgcttat aaatacttcc agaggggcag ttattgatga aaaagaactg attaaagcgc
     1801 tttctgaaaa acgcattttt tccgcaggac tggatgttta cgaaaatgaa cccgatatcc
     1861 cccaggaact tttggctttg gaaaatgtgg ttttactacc tcatatcggc tctgccagca
     1921 ttgaaaccag aaccaaaatg gcacttttgg cggcagaaaa tgccattgcc gttatggaag
     1981 gaagaaaacc accagctcaa gtaaattaag taacacggaa ttgactaact actcggatta
     2041 atataaattg catatttata tcgggacttg caagagaaaa attataaaaa atgattaaaa
     2101 cttgacaaaa aagggagggt gatttattag ataacatata aggttaataa agttagaaga
     2161 tgtgaggtat atatatgcag gaaacaatgc aaaagaccaa tatagggata ttcagtggcc
     2221 tctctgaaga agtagtggaa gaactgacta aagatttgga gcggattaaa tttaagaaag
     2281 gagaggcaat aattactgaa cattccctca gcgataatct ctttttcatc tctaagggca
     2341 aggtggaaat agacaaggac ttaagcaatc ccgacatacc tttcactcag ctctcaacct
     2401 tgggggcggg agattttttt ggcgagatgg gcattattga agatagtact cgttctgcca
     2461 gtgttgtagc tacagaagat gtagagctaa tggttattcc ccgggaggca tttaaggaaa
     2521 tgctctttgc ttatccgata ataatgttga atttaacaaa ggtaatttcc agtcgcttgc
     2581 gtaatactaa tgaaagattt gtagaactga tggatgaaat gctgaaaaaa aaccgtttaa
     2641 tggctattgg attggcagcc agcaaaatta ttcacgatat taaaaccccc ttaacagtta
     2701 ttgtgctaac ggcacagttg ctggagaata tttttcccga aagtgctgaa tttacggata
     2761 acattgttaa acagacaaaa ctgatagacc agctggtgca tgaaattttg gattttgcca
     2821 gaggaacaga aagtcctccc ctgattcaga aagttaattt ggatagcttt ttacaggaga
     2881 taagtgagct ttattccgct tctcttaaag aacgcaagat caattttgta gtggaaaaca
     2941 aggttaatga ttgtgtctat tttgatgaag gtaaaattcg cagggtctta ataaacctca
     3001 ttaaaaatgc cttggaagcc gtttctgaag caggtgaaat caagattatc agcagtgttt
     3061 cttccgggtg gttacagatt tcggtaattg ataacggacc aggtgtttcc caaaaagtga
     3121 aggaagaatt atttcagccc ttcgttacgg atggtaaaac acagggaacg ggtttaggtt
     3181 tggctatatg taaaaaactt gttcaggagc atcacgggcg tttggaatat attcccgtag
     3241 aacctcatgg cagtagattt gatattcgta ttccgcagaa cactaaataa ttgcttgtca
     3301 aaaacagagc cctctttttt ttggtcttta tcataagaaa aaacgggaag tgaattatgt
     3361 ccaaagtatg tgatatatgt ggaaaaacag ctcaggtagg taatcatcgc agtcatgctt
     3421 taaatgctac caagcgtagg ttttatccta atttgcacga aattcgtgtt gccggtgatg
     3481 gcggaactaa gaaaattaag gtttgcagtt cttgcctgaa agcaaataaa gtccgcaaag
     3541 ccgtataata acaatcggtt ttttggctta taatcaagca gagaaatatg tttatcggga
     3601 atcgcggttt tgccggattc ccgatttttg ggttaataaa ggcataacta aagcactaaa
     3661 taactatcta accaataaac ttttatgggg gtttaatgtt aattgaggca atccaaaacg
     3721 aggtagaaaa aagttctgct gtattgggta atgtgcgttc tgaaattgcc agagtgatag
     3781 ttggtcaaca ggaaattata gaccgcttgc tgattggaat tatagcaaat ggtcacattt
     3841 tgattgaagg tgttccaggt ttggcaaaga ccctgattat ttcatcgctg gcatccgttt
     3901 ttgatgcttc ctattcccgc attcagttta ctccggattt gttgcctgca gatataacag
     3961 gcacaatgat ttacaatcct aaaagcgggg attttagcgt gaaaaaaggt cctgtctttg
     4021 ccaatttcat cttagcggat gaaattaaca gagctccttc caaagtgcaa tcggctcttt
     4081 tggaagcaat gcaggaacgg caggttactt taggggataa cacttttcct ttgcccaatc
     4141 cgttttttgt gatggcaact caaaatccta ttgagcagga aggaacttat ccgcttccgg
     4201 aagcgcagat agaccggttt tttatgaagc tgaagattaa atatcctgcc tatgatgaag
     4261 aaaggcaaat cctggaaaga atggttaatg aacagcccat tcccttgcag aaagtgctaa
     4321 gtcccaaaga tattgaagaa atgagggaag tgatgcataa ggtgtatatg gaagaaaagc
     4381 tgaaggatta tattttacat ctcgtcaatt caacccgcca tccggaacgc tatccgcgtt
     4441 taaacgattt gaagggtctg attgaatttg gtgcttctcc ccgagcaaca attttcctgg
     4501 caagagctgc taaaacgaat gcttacattt caggtagggc ttatgtaatt cccgatgaca
     4561 taaaaatgat agcaaaagat gttttaaggc atcgtattat tctttcctac gaagctgaag
     4621 ccgaagaact taccagcgaa gatatcatca atcgcattct ggacgaaata gaagttccgt
     4681 aaaatatagg ggattatgtt tacccaaagc gtttctgata tcctgaaaaa gattcgcagg
     4741 atagaaattc gcactcgcaa tcctgttgcg gaaatattca gaggagaata tcacagtcgg
     4801 ttcaaaggtc agggtttgga attttctgag gtgcgtgaat atcaaccggg agataattac
     4861 agagatattg actggaatgt ttctgcgcgt ttgggacaac tttacattaa aaaatatcgg
     4921 gaaaccagag agttgagggt ggttttctta gtggatgtta gtgcttcaca ggaatttggc
     4981 actcgcagta tgattaaaag agaaaggatt gcagaactgg ttgcagcttt ggctttttca
     5041 gccgttgcta atcaggattt ggctggcctt atcctttttt cggacagagt ggaaaagttt
     5101 ctgccacccc gtaagggacg gaataacgct ttggcaatcc tcagagaagt tctctattat
     5161 gaacccaaaa gcaaaaagac ctctttgcag gttgcttgtg aatatgccca taaaatgctg
     5221 aaaaaacgct gtattctgtt tattatttcg gattggatag attctggtta tgaacattcc
     5281 ctcaaaattc tggctaccaa gaatgatgta attgctataa gagttttgga tgatagcgaa
     5341 ctggaacttc cccgggcagg cattttatct ttgcaagacc cggaaacagg agaggaagtt
     5401 tggataaaca gttccgattc caaactgcgt aaagcatatc aggaaatagt aaataaagaa
     5461 cagaaggact tggaagaatt ctttcgtaaa tgcaattgtg actatttgct tatccgcact
     5521 tccgagtcct atatcaattc cttaagaaag ttcttcactt taagggaaag gagaagatga
     5581 aaagaataaa agtaattgtg tcttttctgt ttttattgct tcccttattt atccgggctg
     5641 aattaagtca ggaacttcag ggtgtggata gtttaaaagt aggcactcct tttctcttta
     5701 aaattactac cgattttgcc ataaaaggtg tttccatacc cgatacatta gaggattttg
     5761 ctattctgga aagtaaacgg gatgaaaaaa atccccgttg ctggataata aaaatagttc
     5821 ccctaaatac aggtgcttta tcctttcccc ggatagaaat acaacctgtt gacagtagtg
     5881 taactcctgt ttacacagat ggcttcagaa tttatgtgct ttctgtttta gcggaaggag
     5941 atactctttt acgggatatt aagcccctga aaaagtattc ccttcaaccg ccattctggg
     6001 tttatctatt gctgattttg cttgctttgg cagggggaat ttatctttgg ttgaagcgac
     6061 cagaaaaacc caaacgggaa tatattgctc aaccggtgat tagagaaccg gttttacctg
     6121 cctgggaaaa ggcattaaaa gcattggatg aacttatagc agagaatttg cctgcgaaag
     6181 gagaaacggt tttataccat ttccgtctat ctaatatttt gcgtgctttt ttggaagaga
     6241 cctatcgttt tcccgctctg gaaatgacag gaagggaaat tgcagttagt ttaaaccgga
     6301 gaaatatccc caaagcacag gaaataagga agtttattaa ttattgtgat ttggttaagt
     6361 ttgccaagcg tgaaccttct gccggtgaaa ttgaagaaaa cacattatgg ttgaggaact
     6421 atctgctaag tttcagtgaa caaactcggg aaacggaaaa tgcttaaatt cggtcatccc
     6481 tggttgcttt tacttttatt ggtaatacca ttatacctta tttgggaatt tgtctttcaa
     6541 aagaaacggc gtctttattt acctcattct cgggtaaaac agctacgcta tctatcacac
     6601 agtcaatatc cttaccattt tttgtatcct gttttgcgtt cttgcatctt gttattttta
     6661 tgtattgcgg tagcgcaacc gcgttgggga ataaaaaccc gggatttaag taataaaggt
     6721 gtggatattg taatggctat agatatcagt ggttcaatgt tagcaatgga ttttgctcct
     6781 aaaaatcgtt taagtgcagc tgtaagtgta gccaaggatt ttgtgaaaag gcgtcccaat
     6841 gacagatttg gcttagtggc tttttcggaa tatgctttaa cgcaggttcc gttaactttt
     6901 gaccatttgg caatgctaaa ttccctggat aagttaaaag tgaatgaaga agcatcggca
     6961 acagctatcg gaatgggttt ggcaaaagcg gttgcccgtt tgaaaaatag cactgccaaa
     7021 agcaaagtga ttattttgat taccgatgga gttagcaata ccggagaaat agacccttta
     7081 accgcggctg aaatggctaa ggaattggga ataaaagttt atcctattgg agtgggaagt
     7141 aaaggtttag ttccgtttcc ttattctgac cctattttcg gcacgcgtta tattaatacc
     7201 tatattgatc ttgatatgga gaccttggat aaaatagcag cgaccacagg aaccgggaaa
     7261 gcggctttag caaccgatgc taaaggtctt gcagatatta tgaatgaaat tgaccgtctg
     7321 gaaaagaccc tttttacaac tcaatttcgc tataattaca gtgagcagtt tatgccattt
     7381 ttgtggctgg cttttgcctt tttagtgctt gaactgctgt tgaaaatatt tattttacct
     7441 gtgttgccgg aacagttatg aatattgcca atccttctta tctggctctt ttagcgttat
     7501 gcttggtatt tctgctattg ctattcggaa gggaaagaaa attaaagcgg cgttttgccc
     7561 aatatgccga attacattta agggagcatt attatcattc ccattctcct ttttggacgg
     7621 ggttcaagct ttttctttct attttggctt tgggttgtat cataatagcg ctggcaagac
     7681 ctcaatggga ttatgaaaac aaggaattac aaagtagtgg aatggatatt atttttgcca
     7741 ttgatgtttc taaaagtatg gatgctacgg atatgatgcc cagtagattg ttgcgtgcta
     7801 ttttacagat tggcagtttt ttagagcagg ttaaaaacga ccggattgga attattgctt
     7861 ttgccggaac ggcaactttg caatgtccct taacagatga ttatgaagca gttagaattg
     7921 ttctaaatgg cttgaacagt aatacggtag aaatttccgg aaccgatatt ggaagtgctt
     7981 tgcgtttagc agaaaatgcc tttcccgagg gctcaaaatc caaaaccctc gttttgattt
     8041 cggatgggga agaccttcgg cattctgctc tcagagaagc aagaatctta aaaacaaaag
     8101 gtatccgggt ctatactatg ggggttggta gccctgaagg gacaattatt cgccatccgg
     8161 aaaccggtga agaagtgaaa agtaaactgg atgaggcaac ccttcaagaa attgcccgga
     8221 taacggatgg tgaatattat cgtgtaactc ctggcggaga agaaattcag ctgattttga
     8281 aacgcatcta tgaaagcgaa agcactcgca gaggaaagaa agtaaccatt ttaaaggaac
     8341 aatattacct ctttggcatt ttggcgatta tcttattaat gatagaaagt tttatagacc
     8401 cccgtaaacg aaaacgcggt attatggctg cggagaaaag caatgagcac taaaacaagg
     8461 cgtaacatca taataattat agcggttatt ttggctttgc taattctgga attgattttc
     8521 cgacccaagg tagtgcgtca agcatttgca gagccatttt acagggcaaa aaaatatccc
     8581 caagcagaaa acctattccg taaaaattct actaaaaacg atgctcccgc taatgccaat
     8641 ctggcaaaat gtttatatcg gcaaaaccgt tttgcggaag cggattcagc tgccacaatt
     8701 gccttaaata gcgccaagga taaaaaggat attctttacg atcgcggcaa tattgcctat
     8761 aaacaacagg attatagtaa agctctgagg gattataaag aagcactatt attaaatcct
     8821 gaagatgatg atttacgagc aaattatgag cttactttac gcaaaatgaa taaacaaccc
     8881 cctccagcgg ttggattaca acagaataag gacgataaga aaaaggaagc aatccgtaat
     8941 attctggaag gactggataa taaagaaagt acggacagac agcaactgcg ctctaaacaa
     9001 agcttaaaaa cggataaatg gtggtaaaaa tgaaaaaggc acttcttctg gttttgctgt
     9061 ttccccttgt gctttctgct ttgaaagtgg atttatccgt gaataaaaat actatcaatt
     9121 taaatgaaca actggaattg attttaaaga tttctgacga caaccggatt aaagttcaag
     9181 aaccaactcc accttctatt cctttattca gttttcgtaa tatgaccagt tcctcaagca
     9241 gttcggttgt tttggaaggg acaaaattaa tttccgaatt ttcggagacc tatcgtttca
     9301 tctattttcc tttgaagacg ggaacaacta ccattccttc ctttcaggtg aaagtaaatg
     9361 gaaagtatta tgctacgcgt cccatttcta ttgaagttgt taaagcagaa caaaaaactc
     9421 ccggacaaag ttcacctccg gttccgggaa ttataccttt tggctttgac gattcttttt
     9481 ttgggggtga tagagaagga tgggcagggg atacttttat tttggcttta cctaaagtgc
     9541 agaaggtcta tcgtggtttt cccgctattg tttcctatta tctttatacc gatgaaatgg
     9601 ttggttcttt caacctggaa aatgaaattg attcggatgg ctatggaaaa agcacttatg
     9661 aacagccaac gatgttaaat tatgaagaag taactatcaa tggaaaacag tataaaagag
     9721 ctctgattaa aagattagcc cttattccca atagagaagg acgcttacag gcaccgattt
     9781 tagagggtta tgcccgtctt tacggttttg gacttttaaa taaaaatctc caaagcgaag
     9841 gtggcatcat tgatgttttg cctttaccca cgaataatgt gccaccttct tttggaggtg
     9901 cggtaggaaa ttttaagcta agtcattctc tttccaggaa ggaactttcc ttgggagaaa
     9961 caattacttt taccctgaaa attgaaggga ggggtaattt taaccagttt tccgctcctc
    10021 aatttgcttc cggaaaaggt tttcaggttt ccactccaat ggttattgat aatttgaatg
    10081 ccggaattga aggaaccaga acttattatt acactttaat tccgcaggat aaaggggttt
    10141 atgagcttcc cgaactcaat tttgcctggt ttgataacga cagcggcagc tataaaatat
    10201 accgcagtcc caaagaacaa ataaaggttt cttcggcaaa tgtgttaagt tatttcagcc
    10261 gtttaagaca atcccgcaat cctaaagcaa tgttgcctaa aataagcagg gaaagctacc
    10321 cggattatgt tccttatgta aaacagccct ggtattggat tttggtggtt ttgattgttt
    10381 tagcgaccat agttgtttcc tttgtggcag tggagagaaa aatgaaaagg gataatcccg
    10441 aacgctatgc ctcgttaaaa gcggaaaaag tcctcctgca ttatctgaaa ccggcaaccg
    10501 cagctgccaa aaatctttct ctggatttct atcccctggc ggaaaaggca ttgtttcaat
    10561 atcttgcttc caaatataaa ctttccaatc atctttctac acaggaaaaa ctacaagcat
    10621 taagtgaaaa aaatatcccc gaatggttgc tggatgaaat tcaaatcttc ctgtctcaca
    10681 gtcaggcagc aagatttatg ccggaaacag acagagcggt aaatttggaa gaagacctgg
    10741 ctcgtattcg caaaatcttt acaggattca gtcgcttaag taaaaataga aaaaacagga
    10801 gtggcaaatg aaaaagtgta gacagagggc tccgttcctt tcccataata aaaggtctct
    10861 caatttacag agctgcaatg agctccaact gcacagaatt tgtagctaca gcaagctccc
    10921 actactctta gtttttgtaa ttatcctgtt ttgcccaacc ctttttgctc aagctgactc
    10981 tttacaaaca acaaacttta gcggttcagg agagcttact cctttgccgg atactttgga
    11041 taaggcaatt cgccttgcag aacaaaaaaa gaatcccgat ttgttttata atttaggggt
    11101 agaatttttc tcgcggggtg aaattgggat ggctaattta tactttttga aagcattaaa
    11161 tataaattct gcccattcac cggcaagaac caatcttgaa ctttccattc attatagtcc
    11221 ggatgcagat ttgtatcctg aacagctgtt tttggtgcaa gtgctgttta aagtgctaaa
    11281 ttacctttcc gtaaatcgtt tggctatact gggccttgtt tttctatttc tttctgcttt
    11341 atcgctatgc tggttgcttt tttataatcc ggaaaaggaa agagctttgc ctattttaac
    11401 cctaactttt tgtttattgc tttgtgtttc cgcgtttgta accctgggta taaaacttca
    11461 tcaccaacaa cataaccgta gtgctgtagt tattgtgcgc gaaacggaac ttctatctcc
    11521 cgcaaataat aatgttattc agaccgtcca ttcgggattg atagtaaaaa ttgttcaaga
    11581 taacggaaac tattatgtag tgcgcctgcc caataaccaa ttgggacgcc tgaataagga
    11641 agctgtaagg aaagttatct gaaataagca gttagatata cagacataat ttcttcctta
    11701 atagcattgc ttacccattg cttacccatt acggatggca ttagcaatgg gtaagcaatg
    11761 cttcagtaat gctacttggc agaacgagtt atctcctttt ctatgtttaa gatatagtta
    11821 ttttattccg aataatgcct ttgaggttaa atgggttggc aacctctttc tttgctaaag
    11881 aattcacaga attctggaaa atgaatgtcc tctatttat
//
LOCUS       DAOOZV010000055        11500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5523_length_11500_cov_59.434163, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000055 DAOOZV010000000
VERSION     DAOOZV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_11500_cov_59.434163"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLW08_06185"
     CDS             <1..488
                     /locus_tag="PLW08_06185"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPL52226.1"
                     /translation="LSIIIIAAYDLNHLIGKNGILPWKITEDLKRFKAITMGHPIIMG
                     KNTWNSIGKPLPGRQNIVLSKDLNFQPQGAIVAHNLEEALKMVEGQETYIIGGASVFK
                     QFLPLADKMLITLIHSEFEGDTYFPYFSEEDWILQESSSLLSSSGYELCLQTYIRKNL
                     L"
     gene            1126..3153
                     /locus_tag="PLW08_06190"
     CDS             1126..3153
                     /locus_tag="PLW08_06190"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPL52227.1"
                     /translation="MKTTYAEIKNRILDCKKPYKTLLQRLNSLDILSDMPYAKFFQDL
                     QNLCLQSDKFAAFLEKDPQLKTIPLDQLSTWHNDYYSALGNYDNCMGNPDYAVKLYGE
                     EMGRFLSALYYQFMLMYVNQATNNYLQMEENLRLFFILYDKAISGNTDYADWLKEYRK
                     IVIENHKTKLYWKLYQNYYPEANINYDLIRNSDLNDVRYLYRYGMFITDASIKMAQFI
                     NNYPEKQLNDIARYIVQCWIDGFIRAGKNYRIKKTANIIVPCGMELLGRLIHQELEKI
                     GIQGQFSVPYKIYENKQFNYDHRFDNALILDRDYTDMVINVYAEVIDDIQELIKGDAG
                     PVYVELFGETPFSPIVKNSVLTLNKEQQELSREMMSKTSQIYYQYYKRDQASFCIIAF
                     PSPEIGEQFQDIFADTLKINLLDSNHYAEIQQKIIDVLDKADYVNVKGKAGNFTDINV
                     KLHTLQNPEKETNFENCVADVNIPVGEVFTSPVLKGTNGTLQVEDIYLGSLRYFNLKI
                     DFEDGWIKDYSCTNFEDPEEGRKYIYENLLLPHKSLPIGEFAIGTNTLAYQMARKYNI
                     LPLLPILIIEKMGPHFAIGDTCYSHEEDSPHPSFVNGKEMIAVDNEKSITRNEDPLNA
                     YLQKHIDITLPYEMLGSITAVTKNGEKFDIIRDGRFVVPGTEELNIPLQDS"
     gene            complement(3193..4248)
                     /locus_tag="PLW08_06195"
     CDS             complement(3193..4248)
                     /locus_tag="PLW08_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPL52228.1"
                     /translation="MKVWKLIPLLFIVFICSCGKENKKQESLSYKHKLVVYATDEFRK
                     SGLEHSIVPDFQKKHNCQLEVILFRNTAELSKAVKNRDNYGKYDLAIGIDNSFAFSET
                     LAVNFVPPESFDKELLIQETVFDPYLRFIPYAYSNLSLIYNTSLVKTPPQSFGELQDA
                     KYLSQIAICDPQTSGLGRSMLFWSVALFGTQGYEHLWKSLRKNIYKTYSNYNEALETL
                     KRGECSFIIGYNTTPAFLQELDPLNRNFEVSMLKEGSFQYIEAIGIHRGTKKSSLCNK
                     FVDYFLSSEAQKMVVYKLGMFPANRKTLLPMHFSAIPFISYSVNDRLSQATIQEQINA
                     WLDFWERLFGYQIAKNL"
     gene            complement(4249..4998)
                     /locus_tag="PLW08_06200"
     CDS             complement(4249..4998)
                     /locus_tag="PLW08_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL52229.1"
                     /translation="MSSNLFAGIGEYTIFVGKCLGKIPSLFKRYQEFLIQFKRIGFDS
                     LFLILLTAAFTGLVTALQAVYQSKGYVPLNLLSVLIGKSTMIELAPVLTALVLTGKVG
                     ASIAAEIGTMRVSEQIDALESMNIDPYEFLYMPRILAGVLAFPLVTVFANAVGIICAW
                     YFSWLRYGIHYYTFFNNMRSYFIPSDLWGGLAKSFVFGFIITSFACYHGDRCFGGAEG
                     VGKATTATVVYSSVAILVMDFIVAWFLFGGY"
     gene            complement(4988..6364)
                     /gene="dnaB"
                     /locus_tag="PLW08_06205"
     CDS             complement(4988..6364)
                     /gene="dnaB"
                     /locus_tag="PLW08_06205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPL52230.1"
                     /translation="MAKLSEADKLNKAQTERALPADINAEAALLSAMLIDSDVVSKGI
                     EKIKEEYFYRNAHKLIFRTMQELFFEGIEIDQLTLINRLERNNLLEKVGGIPYINEIA
                     DFVVSSANFDYHLNIVTDQALLRHLILASNGIIENCYTAAKPVKTIVDEAEQAIFAIA
                     ELPSHQGFVRFDQVSQEVLDNIDKIASTKIPVTGVSSGFADLDRFTGGFRPGQFIIIA
                     ARPAMGKTSLALNIAANAAVNLNKKVAIFSMEMAADEVIMRMFSSASEVRMDGMLRGY
                     GMNEEKLIRIMQASEVLSAKHIYIDDSGTNTPLDIRAKTRRLAAEIGGLDLVLIDYLQ
                     LMTLTREKNNRQQEISEISRALKILAKDMKIPVVALSQLNRAAEGREDKRPKLADLRE
                     SGAIEQDADLVMFIYRDEYYYPETTQKPGIAEIIISKNRHGATGNLELGFEKEFTLFR
                     PLETKYEQ"
     gene            complement(6369..6848)
                     /gene="coaD"
                     /locus_tag="PLW08_06210"
     CDS             complement(6369..6848)
                     /gene="coaD"
                     /locus_tag="PLW08_06210"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPL52231.1"
                     /translation="MSKAIYPGTFDPFTLGHLNILEKACHIFDEVILAVAEYSGKENL
                     FNLQERYELCKKATEHIPNLVVSTFQGLFVDYAKSQNCKIMIRGMRAVSDFEYELSLA
                     ITNKKLNPEIETIFLVPSLKYMYLSSSMVRQLADLKGPLEDYVPACVAEAMRKKYVS"
     gene            complement(6848..8086)
                     /locus_tag="PLW08_06215"
     CDS             complement(6848..8086)
                     /locus_tag="PLW08_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPL52232.1"
                     /translation="MTAEVTIPRREELFSALEEKEAIIIFAAEEEKLEKFKQDNDFLY
                     VTGLQIPEAIYFGYKGQNGPVEMLFIERSDPEKAIWTGPVMSSEEASKKTGLSQDKIH
                     YLDEFLAILSVYCPVINKIWANIGIQALNKPPTRQLFHLFPIRERYPNIIIESLETLI
                     TPLRKVKSEWELRQLQRAIDITGKAIIDVWEKAEPSMMEYELEAIMFYRLQRMGVKHW
                     GFAPIVGSGINAAILHYEKNECPIESGEVVLMDVGASYLNYSADITRCFPVSGTFSER
                     QKQVYNVVLDVQKKIIEMIKPGVELSILNQTARDLLAKGAIELGLIEHEEDIKKYYMH
                     SISHFLGMDTHDVGGRNAMLEIGNVITVEPGLYIPEEKLGIRIEDDVLVTENGYCVLS
                     QNIPKEISEIEEIRLQAMAK"
     gene            complement(8102..9349)
                     /gene="clpX"
                     /locus_tag="PLW08_06220"
     CDS             complement(8102..9349)
                     /gene="clpX"
                     /locus_tag="PLW08_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPL52233.1"
                     /translation="MDKYRTLTCSFCGRGEHEVKNLIRGIAGNICDECIVMCHNIIDS
                     SKEDIKQEDFEAPIPSRIKAYLDQYVIGQDSAKEIISVAVYNHYKRIFKQKYEDDVDL
                     EKSNILMIGPTGSGKTLIAQTLARFLQVPFAISDATTLTEAGYVGEDVENILVRLLQN
                     ANYDVAKAERGIVYIDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERMDKKSIGFDVELGKKQDESTIFD
                     FTTPQDLLKYGLIPELIGRMPVICTLHELSEDALIDILVKPKNAICKQYQKFFEMDGV
                     RLEFSEEALKEIARTAIKQKTGARGLRSIMEKFMLKIMFSIPDRSDVESCIITPEVVT
                     LSQEPKYGYIKPLNSRNKVANEK"
     gene            complement(9441..10025)
                     /gene="clpP"
                     /locus_tag="PLW08_06225"
     CDS             complement(9441..10025)
                     /gene="clpP"
                     /locus_tag="PLW08_06225"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPL52234.1"
                     /translation="MSFVPIVIEQVGRTERAYDIYSRLLKDRIVFVGGVIEDNLANTV
                     VAQLLHLEGEDPERDIYMYINSPGGIISAGLAIYDTMQYIKPRVSTICIGQAASMAAV
                     LLAAGAPGKRSALPNSRIMIHQPMGGAQGQATDIEIQAKEILYLRGRMNEILHKHSGQ
                     SIEKIMEDTERNYFMSAEEAMEYGIIDEVISVRK"
     gene            complement(10022..11317)
                     /locus_tag="PLW08_06230"
     CDS             complement(10022..11317)
                     /locus_tag="PLW08_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPL52235.1"
                     /translation="MQVEFNQLNAVTKEINLTIPSEEVDKEYEKYLKKSAQEISVPGF
                     RKGKAPLNVVERMYEDKIRNYFYEYYIDEVFSKVVKENEIKYLHYPEVKDVQWEKGND
                     MTIKIEIEHEPVLEFKQIEGLTVPYKPMVLEEEVNNFIKELQKSNCRIIDVETARETD
                     NVNVEITFQHNNETFTRTGSFFAGTKHNLDMLPELLGLKIGDKIKVKLTGREIINSTQ
                     DYKLPLDNDAEYDCELMVNSISRIEYPELDDEFAKDMEFDSLEDMKAKISDDLKLKIE
                     HSNIDIENEAIIAKLFADNEFALPKKTLSYLAEEQVKDIKNDNFMQYYYERYYITFAY
                     ALLSLYITNNLLRFMPIELTDEMKEEYINHLAIVKDMSSDAYKEKYKDDISSENFARD
                     AQRYFVLRKIAQTCEFVIKEEPEENSYPESEIETIKEEQ"
     gene            complement(11350..11436)
                     /locus_tag="PLW08_06235"
     tRNA            complement(11350..11436)
                     /locus_tag="PLW08_06235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11400..11402),aa:Leu,seq:tag)
BASE COUNT         3551 a         2271 c         1935 g         3743 t
ORIGIN      
        1 ttctgagtat tataattatt gctgcttatg atctgaacca tctaattggt aaaaacggta
       61 tattgccctg gaaaattacc gaagacctta aaagatttaa agccataaca atggggcatc
      121 ccattattat gggaaaaaat acctggaatt caatcggaaa accattgccc ggaagacaga
      181 atatcgtgct gagcaaagac ctaaactttc aaccccaagg tgctattgtt gctcataatt
      241 tggaggaagc attaaaaatg gtggaaggac aagagacata tattatcggt ggagcaagcg
      301 tgttcaagca atttttacct ctggctgata aaatgctgat aactcttatc cattccgaat
      361 ttgaaggcga cacctatttt ccttattttt cggaagagga ttggattttg caggaaagtt
      421 cctctctgct ttcttcttcc ggttatgaac tatgtcttca aacctatatc aggaaaaatc
      481 ttctctaaaa aaagatcaga gggttgagaa ggttgagagg gttgagaaag tttagatggt
      541 tgagaagatt gagatgccat aaaagcccga agggcgacac aattatagcg atggtggcgt
      601 aagcccctcg ttaaaacgaa agccccgact ttttttccat cttatatttt taccttttct
      661 gtttccaaaa gcccgtcagg gcgacaaaat tatagcgatg gtggcgtaag cccctcgtta
      721 aattcacgct tttcatacta tttggttttt cactcataat tttcacctgc cttcctaaaa
      781 aacacactcc acgaactatt ttacttacaa cttacaaccg agctataatt gctttctgaa
      841 ttaaaaaaat atgacttaaa acaaaaaatg gcgctcaata tattgccact attatcgtta
      901 caagtgcttc cttactcccc actcatcatc cacccatcat ccactcaacc taaacccatc
      961 caaagtttgg gttgtgtatt agagaatata aagtggtatt tgagtaggga gtagggaaga
     1021 agatggaatt gtgcttgaca cattttcttg cctccaaaat agataaattt tcaaaaaggg
     1081 tgagcgtatt ttgctcccag aatgtactat aataaggaaa ccaatatgaa aacaacttat
     1141 gcagaaatca aaaaccgcat ccttgactgt aaaaagccat ataagacatt gttacaacgg
     1201 cttaattcac ttgatatttt atctgatatg ccttatgcca aattttttca ggatttgcag
     1261 aatctttgtt tgcagagtga caagtttgct gctttcctgg aaaaagatcc ccaattaaaa
     1321 acaatccctt tagatcaact ttccacttgg cacaatgact attattctgc tttgggaaat
     1381 tacgataatt gcatgggaaa tcctgattat gcagtaaaac tgtatggtga agagatgggc
     1441 agatttcttt ctgctctcta ttatcaattt atgcttatgt acgtgaatca ggcaactaat
     1501 aactatttac agatggaaga aaatctgcga ttgtttttta tcttatacga caaagctatt
     1561 agcggaaata ctgattatgc ggactggtta aaggaatatc ggaaaattgt gatagaaaac
     1621 cataaaacca aactctattg gaaattatat caaaattact atccggaagc aaatatcaat
     1681 tatgatctta ttaggaattc cgaccttaat gatgtgcgct atctttaccg ctatggaatg
     1741 tttataactg atgcaagcat aaaaatggct caattcatca ataactatcc ggaaaaacaa
     1801 cttaatgata ttgcccgtta tatagttcag tgttggattg acggttttat tagagccgga
     1861 aaaaattatc ggataaaaaa gacagctaat ataattgttc cttgtggaat ggaacttttg
     1921 ggacgcctta ttcatcagga actggaaaag ataggtattc aagggcaatt ttctgttcct
     1981 tataagatat atgaaaacaa gcagtttaat tatgatcacc gttttgataa tgccctgatt
     2041 ttagataggg actatacaga tatggtaatt aatgtttatg ctgaagtgat agatgatatt
     2101 caagaactga ttaaaggtga tgccggtcct gtttatgtag aactttttgg tgaaacacct
     2161 ttttctccga tagtaaaaaa cagtgtttta actttaaaca aagaacagca ggaactttcc
     2221 cgggaaatga tgtccaagac ctctcaaatt tattatcaat attataaacg agaccaagcc
     2281 agtttttgca ttatcgcttt tccttcaccg gaaattggtg aacagttcca ggatattttt
     2341 gccgatacct tgaaaatcaa tcttttggac agtaatcatt atgcggagat tcagcagaag
     2401 ataattgatg tgcttgataa agctgattat gtgaatgtga aaggaaaagc gggtaatttc
     2461 acagatatca atgttaaatt gcatacctta caaaatccag aaaaggaaac caactttgaa
     2521 aattgtgtgg cagatgttaa tattcctgta ggtgaggttt tcacttctcc cgtgttaaaa
     2581 ggcactaacg gaacattaca ggtggaagat atttatttgg gttccttacg ctattttaat
     2641 ttgaaaattg actttgaaga cggatggata aaggattatt cctgcactaa ttttgaagac
     2701 ccggaagaag gcaggaaata tatttatgaa aacctgcttt taccccataa atcacttccg
     2761 attggtgaat ttgctatagg cacaaacact ttggcttatc agatggcacg aaaatataat
     2821 attctgcctc ttctgccaat tcttataata gagaaaatgg gacctcactt tgctattgga
     2881 gatacctgtt acagccatga ggaagatagt ccccatccca gttttgtaaa cggtaaagaa
     2941 atgattgctg tagataacga aaaatccatt acccgtaatg aagatccgct taatgcttat
     3001 ctgcaaaaac atatagatat taccttgccc tacgaaatgc tgggctccat aactgccgtc
     3061 actaaaaacg gagaaaaatt tgatattatc agagatggca gatttgttgt tcccggcact
     3121 gaagaattaa atatccctct tcaggatagt tgatgtcctc gtaaccaatg aacgaggagg
     3181 acatcgtttt tcctataagt tcttagctat ctggtatcca aaaaggcgtt cccaaaaatc
     3241 cagccaggca ttaatctgtt cttgaatagt agcttgtgaa aggcgatcat ttacactgta
     3301 actgataaaa ggaatggcgg aaaaatgcat tggcaaaagg gttttacgat tagcaggaaa
     3361 cattcccagt ttgtaaacta ccattttttg tgcttcggag gaaagaaagt aatcaacaaa
     3421 cttgttgcat aaagaggatt tcttagttcc acgatgaatt ccgattgctt caatatattg
     3481 aaaactgcct tctttcagca ttgagacctc aaaattacga tttaaaggat caagttcctg
     3541 taaaaaggca ggggttgtat tataaccaat aataaaagaa cattcacctc ttttcagtgt
     3601 ctccagtgct tcattatagt tagaatatgt tttatagata tttttccgta agctcttcca
     3661 taaatgctca taaccttgtg ttccaaaaag agctacactc caaaaaagca tagaccgtcc
     3721 caaacctgag gtctgaggat cacaaattgc tatttgagaa aggtatttgg catcttgcaa
     3781 ttcaccaaat gattgaggag gagtttttac taaagaagta ttataaatta gggacaaatt
     3841 gctataagca taaggaataa agcgtaaata agggtcaaaa acggtttctt gaattaaaag
     3901 ctctttatca aatgattccg gtggtacaaa attgactgcc agagtttcag aaaaggcaaa
     3961 actgttgtca attccaatag caagatcata ttttccataa ttgtctctgt ttttaactgc
     4021 tttgctcaat tctgcagtat tacggaaaag gattacctcc agctggcagt tatgcttttt
     4081 ttgaaaatca ggaacaatgg aatgctccaa acccgattta cgaaattcat cagtagcata
     4141 aacaaccagt ttgtgtttgt aggataaact ttcctgtttt ttgttttctt ttccgcaaga
     4201 gcatataaaa acgataaaca ataaaggtat cagtttccag accttcaatt aatatcctcc
     4261 aaacaaaaac caggcaacaa tgaaatccat taccaaaatg gctacggaag agtaaaccac
     4321 tgtagctgtt gtggctttac caactccttc cgctccccca aaacagcggt ctccatgata
     4381 acaggcaaaa cttgtaatta taaaaccaaa aacaaaggac ttggctaagc cgccccaaag
     4441 gtctgaagga ataaaataac tgcgcatatt attgaaaaaa gtgtaataat gaatcccata
     4501 acgcaaccag gaaaaatacc aggcacaaat tattcctaca gcattggcaa aaacagtaac
     4561 taaaggaaat gccaaaacac cagcaagaat cctgggcata taaagaaatt cataaggatc
     4621 aatgttcata ctttccaaag catcaatctg ttcgctaact ctcattgtgc caatttcagc
     4681 tgctatggaa gctccaactt ttccggttaa aactaaggca gttaaaaccg gtgccagttc
     4741 tatcattgtg gatttgccaa tgagcacact taaaagattt aagggcacat aacctttgga
     4801 ttgataaact gcctgcaatg cagttaccag gcctgtaaat gcagcagtta ataaaattaa
     4861 aaaaagggaa tcaaaaccaa tacgcttaaa ttggattaga aattcctgat agcgtttgaa
     4921 cagcgaggga attttgccca gacacttacc gacaaagata gtatattctc caatgccggc
     4981 aaaaagatta ctgctcatat tttgtttcaa gaggacggaa aagggtaaat tccttttcaa
     5041 atcccagttc cagatttcct gtagctccgt gtctgttttt actgataatt atttctgcta
     5101 ttcctggttt ttgtgttgtt tcaggataat aatattcatc gcgatagata aacattacca
     5161 aatcggcatc ttgttcaata gctcccgatt cacgtaagtc cgccagtttg ggtcttttat
     5221 cttccctgcc ttccgcagct ctattaagtt gagaaagtgc caccaccgga attttcatat
     5281 ctttggctaa aatctttaaa gctcttgata tttcagatat ttcctgctgt ctattatttt
     5341 tttcgcgggt gagagtcatc aattgtaaat agtcaattaa aacaaggtct aaacccccga
     5401 tttcagctgc cagccgtctg gttttagctc taatatccaa aggagtattg gttcctgaat
     5461 catcaatgta aatatgtttg gcagaaagca cttccgacgc ttgcataatg cgaataagct
     5521 tttcttcatt cattccataa ccgcgtaaca taccatccat tctaacttcg gaagcagaac
     5581 tgaacatccg cataataact tcatcggcag ccatttccat agagaagata gcaactttct
     5641 tgttcaaatt taccgctgca ttagctgcaa tatttaaagc caaagaggtt tttcccattg
     5701 ccggacgcgc agcaataata ataaattgtc cgggtctgaa accacctgta aaacggtcta
     5761 aatcggcaaa accactactc acaccagtaa caggaatttt ggtagaagca attttatcta
     5821 tattatccag aacttcctgg ctgacttgat caaaacgcac aaaaccttga tgactgggta
     5881 attcagcaat ggcaaaaatt gcctgttctg cttcatctac aatggtttta acaggtttgg
     5941 ctgcagtgta acaattctca ataattccgt tggaagctaa aatcaaatga cgcaataatg
     6001 cctggtcggt tacgatatta agatgataat caaaattggc agaagaaacc acaaaatccg
     6061 caatttcgtt aatataagga attcctccta ctttttccag aaggttattt ctttccagac
     6121 ggttaattaa agtcaactgg tcaatctcaa ttccttcaaa aaagagctcc tgcatagtgc
     6181 ggaagattaa tttatgtgca ttacgataaa aatattcttc ttttatcttt tcaatacctt
     6241 tgctgactac atcactgtca atcagcatag ctgaaagcaa agctgcttca gcattaatat
     6301 cggcaggcag agctctttcc gtctgtgctt tattcaattt atccgcttcg ctcaattttg
     6361 ccataacttt agctcacata tttttttctc attgcttctg caacgcaggc aggaacataa
     6421 tcctccaaag gtccttttaa atcggctaac tgacgaacca ttgaagatga aagatacata
     6481 tattttaaag aaggaaccaa aaagatagtt tctatttccg gattaagttt cttattagta
     6541 attgccagag aaagttcata ttcaaaatca gagaccgctc tcattccgcg aatcattatt
     6601 ttgcaattct gacttttggc ataatccaca aacaatccct ggaaagtgct gactactaaa
     6661 ttggggatat gttcagtggc ttttttacac aattcatagc gttcctgcaa attaaacaga
     6721 ttttccttgc cgctatattc agcaacagca agaattactt catcaaaaat gtggcaggcc
     6781 ttttccagta tattcaaatg acccaaggtg aagggatcaa aagttccagg atagattgct
     6841 ttagacatta tttagccatt gcctgcaagc ggatttcttc aatttccgaa atctccttgg
     6901 gaatgttttg ggaaagaaca caataaccgt tttcggttac taaaacatca tcctcaatac
     6961 gaattccaag tttttcttcc ggaatataga gtcccggttc aacagtaatt acattaccta
     7021 tttccagcat agcattacga cctcctacat catgagtatc cattcccaaa aagtgactga
     7081 tgctgtgcat ataatatttt ttaatatctt cttcgtgttc aattaacccg agttctattg
     7141 ctccttttgc taaaaggtcg cgtgctgttt gatttaagat tgaaagttct acacctggct
     7201 taatcatttc aataatcttt ttctgcacat ctaaaacaac attataaacc tgtttctggc
     7261 gttcactgaa agttccgcta acaggaaaac aacgggttat atctgcactg taatttagat
     7321 aggaagcacc tacatccatt aaaacaactt ctccgctttc aatagggcat tcatttttct
     7381 cataatgcaa tattgccgca ttgattccac tacctacaat aggagcaaaa ccccaatgtt
     7441 taactcccat tctttgcaaa cggtaaaaca ttattgcttc cagttcatat tccatcattg
     7501 agggttccgc tttttcccaa acatctataa tagctttacc ggttatatca attgcccttt
     7561 gtaactgcct caattcccat tcacttttca ctttccgcaa aggagttatc agtgtttcaa
     7621 gggattcaat gattatattg ggatagcgtt cgcggatagg gaagaggtga aaaagttgtc
     7681 ttgtaggcgg tttatttaat gcctgaattc cgatattagc ccatatttta tttataacag
     7741 gacagtaaac ggaaagaata gctaaaaact catccagata atgaatttta tcctgtgaca
     7801 aaccggtttt tttactggct tcttcactgc tcattacagg tcctgtccaa atcgcttttt
     7861 ccggatcact gcgttcaata aaaagcattt ctacaggtcc gttctgtcct ttgtagccaa
     7921 aatagattgc ttccggaatt tgtaaaccgg ttacatagag gaaatcatta tcctgtttga
     7981 atttttctaa tttttcctct tctgctgcga aaataattat agcttctttt tcttctaatg
     8041 cagaaaatag ttcttcccgg cgtggaatag taacttcagc cgtcattatc gggtctccgt
     8101 tttatttttc attagcaact ttattgcgag aattgagggg cttaatatag ccatatttgg
     8161 gttcttggga gagagttaca acttccggag tgataataca actttctaca tcagagcgat
     8221 ctggaatact aaacattatt ttcagcataa atttttccat aatagaacgc aaaccccggg
     8281 cacctgtttt ctgctttata gcggttcttg ctatttcttt taatgcttct tctgaaaatt
     8341 ccaggcggac tccatccatt tcaaagaact tttgatattg cttgcagata gcatttttgg
     8401 gctttacaag aatgtctatc aaggcatctt cgctaagttc atgtaaagtg cagattaccg
     8461 gcattctacc aataagttca ggaatcaagc catattttaa caggtcttga ggagttgtaa
     8521 aatcaaagat tgtggactca tcctgttttt tacccaattc tacatcaaaa ccaatggatt
     8581 tcttatccat cctttctttg atgatttttt ccaaaccgaa gaaagctcct cccgcaataa
     8641 acaggatatt tttggtgtcc atctcaataa aatcctgttg aggatgtttg cgtccacctt
     8701 tgggaggaac atttactttt gtgccttcca aaattttaag tagcgcttgc tgaactcctt
     8761 caccgctaac atcacgcgtt atggaagggg tttcggattt acggctgatt ttatcaattt
     8821 catctatata gacgattccc cgttcagctt tggcaacatc ataattggca ttttgcagta
     8881 aacgcaccaa gatgttttct acatcttcac ctacataacc cgcttcggtt aaagttgtgg
     8941 catcggaaat agcgaaagga acttgtaaaa accttgccag tgtttgagca ataagggttt
     9001 taccgcttcc tgtgggacca atcatcaaga tattactttt ttccaaatcc acatcatctt
     9061 catatttctg tttgaatatg cgcttgtaat gattatagac cgctacagaa attatttcct
     9121 tggcactgtc ttgaccgatt acatattgat caagatatgc ctttattcta ctgggaatag
     9181 gtgcttcaaa atcctcttgt tttatgtctt ctttactgga atcaattata ttgtggcaca
     9241 ttacaataca ttcatcacaa atgttgcctg caatacctct aatcagattt tttacttcat
     9301 gttcgcctct tccgcaaaag gaacaggtta atgttctgta tttatccaag gaatcctcct
     9361 ttttaattta ctttctttac ttcaaaattt tatccgcttt ctgtaagtca tttttatctg
     9421 actgcttaaa tttagtcaag ctattttcgc actgaaatca cttcatctat aataccatat
     9481 tccattgctt cttcagcact cataaaataa ttcctttcag tatcttccat aattttttca
     9541 atgctctgac cgctatgttt atgcagaatt tcattcatcc tgccacgtaa atagagaatt
     9601 tcttttgcct gaatttcaat atcggttgct tgaccttgtg ctcctcccat tggttggtga
     9661 atcataattc tgctatttgg cagagcgctt ctttttccag gagcacctgc tgccaataaa
     9721 acagctgcca tacttgctgc ctgaccaata caaatagtac ttacgcgggg ttttatgtat
     9781 tgcattgtat cgtaaattgc aagtcctgca gaaataattc ccccgggact gtttatatac
     9841 atataaatat cccgttcagg atcttcacct tctaaatgaa gtaattgggc aacaacagta
     9901 tttgccaaat tatcttctat tactccaccg acaaagacaa ttctgtcttt cagcaaacgc
     9961 gaataaatgt cataagcacg ttctgttctg cctacttgtt ctataactat agggacaaaa
    10021 ctcattgttc ctccttaatt gtttctatct cggattccgg gtagctattt tcctcgggtt
    10081 cttctttaat aacgaactca caggtttgag cgatttttct taagacaaag tatctttgag
    10141 cgtcacgagc aaaattttca gaagaaatat catctttata tttttcttta tatgcatcgg
    10201 agctcatatc ttttacaata gccaagtggt ttatatattc ttctttcatt tcgtcagtaa
    10261 gttcaatagg cataaacctt aatagattat tcgtaatata caatgacaat agagcataag
    10321 caaaagtaat atagtagcgc tcataataat actgcataaa attatcgttt ttaatatcct
    10381 taacttgttc ttccgcaaga taggatagag tttttttggg gagagcaaac tcattatcag
    10441 caaaaagctt ggctataata gcttcgtttt ctatatctat gtttgaatgt tcaattttca
    10501 attttaaatc atcactgatt ttcgctttca tatcttccag agaatcaaat tccatatcct
    10561 tggcaaattc atcatccagt tccggatatt ctattctgga aatgctattc accatcaatt
    10621 cgcaatcgta ttctgcatca ttatccaaag gaagtttata atcctgagta gaattaataa
    10681 tttctctacc tgttaatttt actttaattt tgtcgccaat ttttagaccc agtagttccg
    10741 gcagcatatc caggttatgt tttgttccag caaaaaaact gcctgttctg gtaaatgttt
    10801 cattattatg ttgaaaagta atctctacat ttacattatc tgtttccctt gctgtttcta
    10861 catcaattat ccggcaattt gatttttgta attctttaat aaaattgttt acttcttctt
    10921 ccaacaccat cggtttatat ggaacagtta aaccttctat ctgtttgaat tcaagaacag
    10981 gttcatgttc aatctcaatc ttgattgtca tatcattacc tttttcccat tgaacatctt
    11041 ttacttccgg ataatgaaga tattttatct cattttcttt aacaacttta ctgaagacct
    11101 catctatata atactcataa aaataattcc gaattttgtc ttcatacatt cgttctacaa
    11161 cattcagagg tgctttacct ttccggaaac cgggaacgct tatttcctgg gctgactttt
    11221 tcaaatattt ttcgtattcc ttatcaactt cctcggaagg aattgtaaga ttaatttcct
    11281 ttgttactgc atttaattga ttgaattcta cttgcacttt attctcctaa ttttatatca
    11341 tccaaaaaat ggtgcgaaag aggggacttg aacccctatg agtttaacct cactggatcc
    11401 taagtccagc gcgtctgcca attccgccac tttcgcattt atttttcctt aaataaaact
    11461 aaaaatattt accttgccct tctgtcaagt gctatgtatt
//
LOCUS       DAOOZV010000056        11470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5544_length_11470_cov_63.888392, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000056 DAOOZV010000000
VERSION     DAOOZV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_11470_cov_63.888392"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..260)
                     /locus_tag="PLW08_06240"
     tRNA            complement(184..260)
                     /locus_tag="PLW08_06240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Val,seq:cac)
     gene            complement(354..430)
                     /locus_tag="PLW08_06245"
     tRNA            complement(354..430)
                     /locus_tag="PLW08_06245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Val,seq:gac)
     gene            complement(513..1853)
                     /locus_tag="PLW08_06250"
     CDS             complement(513..1853)
                     /locus_tag="PLW08_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52236.1"
                     /translation="MKYIYLIFALLACSILFAEPTYMPVTTIAEDFGATWCLNCPLAW
                     QGLQAVHSVTHNGEFISARLFTESGSLSNYMVDERFDYYNITGLPNVIFNGKISLEGI
                     GAGTADGTDYLNALSVYRYSASPVKIVPTNFNFERGLISGSVIMISPDVTISDARLVY
                     YLLEDNVGEEDHIVRGVLYDENVSLSGVGDTYNFNQTFDLEPSWVVSNLWGIVFLQLP
                     NKTILQCISSLPLPEYNFRAAMDWSRYITGDANTNYLSAPLWFFNLGAAENYTMHLEA
                     DNAPADWYFNYCDETGNCYPGSIDRPFSMSAGEALSFHLNLTVGSSAIVDFRFVVSSP
                     HIGSYSIPFRYDAGTPVIDETNNPGALMINNLYPNPVSGTAVFRLLSPKNNDSATIEI
                     YNVKGQKVQCLTAINLHNGINEISFVPDSSLPNGVYFYRLQNTNSKVGRFLLLQ"
     gene            complement(1987..3429)
                     /locus_tag="PLW08_06255"
     CDS             complement(1987..3429)
                     /locus_tag="PLW08_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPL52237.1"
                     /translation="MKHTLFIMLLISFTLLSAQNALSVNGSNEAKFIYRTVEDSLHTY
                     FNDTFGFNLAYRNFSFGMKFIAELPKYSTEQTELLDELDANRLELGWQELYASYEKDA
                     CKIYAGITEESFGNGIVFRSYKDLEFDVDNRLESVLFSYNDAFKFKAIYGAIENPAIV
                     GKYDLAYGADIQSPYIQGLSLGASAMAFRNLLATNIYNQRTVFAGRLNYASKYVDFQA
                     EMAFSELYHQPGIGTKNGKAIYVNGSYYFGPVTLGGAYKQYDKFQYRLNDLPAVNYHN
                     ETLSDASATGEDEIGYQSFGTVNITDYLNFTADYAEAWNSDKNKKMNDAYIALEYVKD
                     SFSLLASYSHIEKIDDISNSWQQDLIPALQTNFALLKIPVQIQVDYKKVSKQRQEAES
                     EHFEPKLQTDFTLGKLSLSLCAQSNWEDISEILDSRYWASAQIKYPLFEHSDIILFGG
                     KEAGGKVCRNGVCRYVAPFEGLRFELNTRF"
     gene            complement(3695..4186)
                     /locus_tag="PLW08_06260"
     CDS             complement(3695..4186)
                     /locus_tag="PLW08_06260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPL52238.1"
                     /translation="MKKPIACLLLLLLVSFAMADMMPDFKLPDATGKNVILQELLGKG
                     PVLIDFWADYCKPCKDAMPQLNTLAEKYDSLTVVMISIDAPKNLPKAKNYLKSKNYKF
                     ITLFDSEKTLAKKLGVTNPPHTFILNKTGEIVYSHIGYEPGVENEYEMHIRNLLGLET
                     EAE"
     gene            complement(4183..5307)
                     /locus_tag="PLW08_06265"
     CDS             complement(4183..5307)
                     /locus_tag="PLW08_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52239.1"
                     /translation="MKKKIVYALLIVALLGIFSCDRFDHNFKPLEQVDFNAELFSPLE
                     EAFNTASAEDLSTVMAFYAEDYKHYGITKSEWEAILYNLLSGINNPFIDVTLITATLQ
                     NETNALANWRLTISDSRKNVIADSLYTGERLKKENGKWLLRGNQCGCDPSAPTQKVIV
                     EYVTNVGCSYCPAVENLLHDLSSQYGNDFIYLTHQLSGPVAFNDPLYAYYSAYSAPVS
                     IIQGKHKLVGGNQETLNQYPYIVQSELELLSQMDYSVLNATVEGKNISGSVKLTPLST
                     SFNQEELILNLAVIDLVSTALNAEGEHLTNVVIGRKRIDISEVDLSNPVAFEFDANVT
                     IPEDAALVIFTQKTPATFANNATIYSGLQYPLAVKKGGSQ"
     gene            complement(5326..7131)
                     /locus_tag="PLW08_06270"
     CDS             complement(5326..7131)
                     /locus_tag="PLW08_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPL52240.1"
                     /translation="MFCLVLLILPIFAQSVKFSISPEVLKPGEKGVIKATLLIPEGKK
                     QTVNPQNPEYFYLEASHPDLIWGKVIYPKPTQVVSEEEWNYHPQVTLTLPFTVKQTAK
                     TGNKQIEVLLSYNLCYETGMCDPPEEATAILSIKIVGNEPIPAAVSENEEEIPNTIEQ
                     KETIPIEKSPIPDKKSETSHPLGEILKYILFAFLGGIILNITPCVLPILPIRIMSIMN
                     QAQKDKTKVLNHTLIYALGVLISFAVMAGIFIALQKAGESVGWGLQNQNPGFVVTLMS
                     IVFVFALSLLGVFEITVPGMNTANKATSRGGYSGSFFGGIFAFLMAISCTGPFLGAAL
                     PFALALPPVLMLIFFLTIGLGFAFPFLLIGFFPKALKIIPKPGNWMIIFKEVMGFVLL
                     IIVYTQLKTLLLLTDGEYLMKVLWFLFILGFSTWLYGRFVRVENSKLTQWLFTLISIG
                     LIIFAAVTYLPIKEAKQEEIQVKSGELIPAPNAPEGWYVFSEDILNKALKEGRAVFLD
                     IGAAWCKNCMTNEKTVLFTETMMQEFKKKNVLLLRGDFTKKDETLLAWIKKHGRAGVP
                     FNALYIPGAEPHIFGELLSKDEVINALNKIPAQAE"
     gene            complement(7388..7942)
                     /gene="frr"
                     /locus_tag="PLW08_06275"
     CDS             complement(7388..7942)
                     /gene="frr"
                     /locus_tag="PLW08_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPL52241.1"
                     /translation="MENIKDNTKEKMQKSFDAMLHQYSKIRTGRASASILDDIKINYY
                     GLPTPIKQLCNISIPEARMIVIQPWDKTTLADIEKAILAANIGITPENDGNVIRLPFQ
                     PLTEEKRRDIVKNIKKISEDTRVAIRNIRRDANDIVKKEKKDSEISEDEEKKQLKELQ
                     DLTDDWIKKIDEAEKAKEKEIMEV"
     gene            complement(7973..8701)
                     /gene="pyrH"
                     /locus_tag="PLW08_06280"
     CDS             complement(7973..8701)
                     /gene="pyrH"
                     /locus_tag="PLW08_06280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPL52242.1"
                     /translation="MKNVYKSENIHRVLLKLSGEVLSGKKGIIYDETVIDSLTDAIIT
                     VHNQNYELGIVLGGGNIFRGGSWKNKNLNRVVLDSIGMLATIQNSLYMAEILISKGID
                     CAIFSTLAVDKVVDRYTPQLASSALEQGKVCFFSGGTGNPYFTTDTAAVLRAVELQAD
                     IVLKATNVDGLYSADPKKDKNAHFISSASFEECLQKRLGVMDLTAFSLAMDNAMPIKI
                     FNISQPQMLIEALTNAETGTYIHP"
     gene            complement(8752..9351)
                     /gene="tsf"
                     /locus_tag="PLW08_06285"
     CDS             complement(8752..9351)
                     /gene="tsf"
                     /locus_tag="PLW08_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPL52243.1"
                     /translation="MAEITATQVKELRDRTGAGMMDCRKALIEKNGNIDEAIKYLREK
                     GISKAEGKANRATKEGIIHSYIHFNNRIGVLLELNCESDFVARTDEFKALADEIAMQI
                     AATNPLAISPEQIDPAILEREKEIAYNKAVNEGKKPEIIEKIVEGNLKKFCNERSLLG
                     QELISDSTKTVKDLLTNAIATTGENIQIARFVRYQLGGE"
     gene            complement(9394..10215)
                     /gene="rpsB"
                     /locus_tag="PLW08_06290"
     CDS             complement(9394..10215)
                     /gene="rpsB"
                     /locus_tag="PLW08_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPL52244.1"
                     /translation="MSVVTMKQLLEAGVHFGHQTFKWNPKMKKYIFIKRNGIHIIDLK
                     QTVDAINEAYQFMKEVASKGEYILFVGTKKQAQAAIKEAAEKAGVFYVNQRWYGGMLT
                     NMATIRQSIEKMKYYEEIVADGTINGYTKLEQQKMKRMHDKIEFSLGGIREMDALPGC
                     VFIVDTEYEKIAVHEARILNIPIVAMVDTNCDPDLVDYVIPSNDDATRAIHLISDIMA
                     NAVIEGKGLATEGESTEEETTETTAEEETEDFEAELAAAEEKVEFDVPELAETEQ"
     gene            complement(10455..10841)
                     /gene="rpsI"
                     /locus_tag="PLW08_06295"
     CDS             complement(10455..10841)
                     /gene="rpsI"
                     /locus_tag="PLW08_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPL52245.1"
                     /translation="MQTFDAVGRRKNAVARVRLMPGTGKRIINNIQMKKYLQRETLEM
                     IVEQPLQTVGLSDNFDVYVNVYGGGLSGQAGAIRHGISRALVEYDENLRPVLKTRGFL
                     TRDPRMVERKKSGRPKARKRFQFSKR"
     gene            complement(10854..11282)
                     /gene="rplM"
                     /locus_tag="PLW08_06300"
     CDS             complement(10854..11282)
                     /gene="rplM"
                     /locus_tag="PLW08_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPL52246.1"
                     /translation="MKTLTPSPSDIHQQWYIVDAEGKPLGRLSTKVATILCGKHKPYY
                     VPNIDTGDYVIVINAAKVRVTGMKALQKVYKSFSGYPDGLKEIPYTKVLEEHPERIIE
                     HAVKGMMPKNKLGRAMFKKLKVYPGAEHPHSAQKPIELNI"
BASE COUNT         3401 a         2389 c         2076 g         3604 t
ORIGIN      
        1 acgctgttcc tccgcttttt gtgtagttaa tagatgtttt tttccggatg gttgatatcc
       61 ccgtcaacca aaaagcccgt cagagcgaca caattataac gatgggtgtg taaacccctc
      121 gtaaaaaagg tttagatggt tgagattctc tctgccccct gctctctgcc ctctgccaaa
      181 aagtggtggg tgatacagga ttcgaaccag tgacctctac gatgtgaacg tagcgctcta
      241 accagctgag ctaatcaccc ttgcaccttt aaaatgtaaa aatattatat aagagaatgt
      301 ctcaaaagac aaaatactga gctaaccaca taactatcag taacaaaaga agatggtggg
      361 agatactgga ttcgaaccag tgacctctac gatgtcaacg tagcgctcta accaactgag
      421 ctaatcaccc actctttagt cagaattctt aaatggtctt ttttgtcaag ttccgaatta
      481 tgtataagct taaaaaggcg ttataatggc ttttactgca agaggagaaa gcgtcctacc
      541 ttgctgtttg tgttttgcaa gcgataaaaa taaaccccgt tgggaagaga agaatccgga
      601 acaaagctga tttcatttat gccattatgc agattaatag cagttaggca ctgcactttt
      661 tgtcctttca cattataaat ttctatagtt gcagagtcat tatttttagg acttaaaaga
      721 cgaaaaaccg cagttccgga aacgggattg ggatataaat tattaatcat caaggcacct
      781 gggttattag tttcatctat cacaggggtg ccagcatcat aacgaaaagg aatgctgtaa
      841 cttccgatat gaggactact tactacaaaa cggaagtcaa ctatggcaga tgagccaacc
      901 gttaaattca gatgaaagga taaagcttct cccgcagaca ttgaaaaagg acggtcaatg
      961 cttcccggat agcagtttcc cgtttcatca caataattaa aataccagtc cgctggtgca
     1021 ttatccgctt ccaggtgcat agtgtaattt tcagccgctc caagattgaa gaaccacaaa
     1081 ggagctgaaa gatagttagt attggcatct cctgttatat accgactcca gtccatagct
     1141 gctctgaaat tatattccgg cagaggcaga gaagaaatac attgaagaat tgttttattg
     1201 ggaagctgca gaaaaacaat cccccacaag ttacttacca cccaagaagg ttctaagtca
     1261 aatgtctggt taaagttata tgtatctcca acaccactta aagatacatt ttcatcgtaa
     1321 agaacacctc ttacaatatg gtcttcctct cccacattat cttccagcag ataatataca
     1381 agacgagcat cggaaattgt tacatctggc gaaatcatta ttacagaccc gctaatcaaa
     1441 cccctttcaa aattgaaatt agtcggtaca attttgaccg gtgaagcgga ataacggtaa
     1501 actgaaagag cattaagata atcagttcca tctgcagttc ctgctcctat tccctccaga
     1561 gatattttac cgttgaaaat cacattaggc aaaccggtaa tattataata gtcaaacctt
     1621 tcatctacca tataattact gagagacccg ctttccgtaa ataacctggc actaataaat
     1681 tcgccgttat gagtaacgga atgaactgcc tgtaaaccct gccaggcaag aggacaattc
     1741 aaacaccagg tagctccaaa atcctctgct attgttgtta caggcatata agtcggctca
     1801 gcaaaaagta tactgcaagc caaaagagca aatatcagat aaatgtattt cattttcatc
     1861 tcctcgtctt tttatataat aactaattac gggaaggtaa caacaaaata tatctgccct
     1921 cccgtcagga atcctaaccg ctaaataaat ctgcgtaatc tgcgtaatct gcgtgaaata
     1981 ttctttttaa aagcgggtgt taagttcaaa tcttaaacct tcaaagggag caacatagcg
     2041 gcagacacca ttccgacaaa cttttccccc agcttctttt cctccaaaaa gaattatatc
     2101 gctatgttca aaaagcgggt atttaatttg agcggaagcc caatatctgc tgtctaaaat
     2161 ttcgcttata tcttcccagt tgctttgtgc gcataaagaa agtgaaagtt tacccaaagt
     2221 aaagtccgtc tgcagtttgg gttcaaaatg ctcactttcc gcttcctgtc tttgtttgga
     2281 gaccttttta tagtccacct gaatttgcac aggtattttc agcaaggcga agtttgtttg
     2341 caaggcagga attaaatcct gctgccagga attgctgata tcatctattt tttcaatatg
     2401 gctgtaagaa gcaagcagag aaaaggaatc cttaacatat tccagggcaa tgtaggcatc
     2461 gttcattttc ttgtttttat cgctattcca cgcttccgca tagtccgcgg taaaattaag
     2521 ataatccgta atattaacgg ttccaaagct ctgatagcca atttcatctt ctcctgtggc
     2581 agaggcatcg gaaagggttt cattatgata atttactgcc ggcaagtcat ttaaacgata
     2641 ttgaaactta tcatactgtt tataagcacc ccctaaagtt acaggaccga aatagtaact
     2701 gccattcaca taaattgcct ttccgttttt agttccgata ccgggttgat gatataattc
     2761 actgaaagcc atttccgctt gaaaatcaac atattttgaa gcataattca atctgcccgc
     2821 aaaaacagtt cgctgattat agatattggt ggcaagcaaa ttgcggaaag ccatagctga
     2881 agcacctaaa gaaagacctt ggatataagg actttgtata tcggcaccat aagccagatc
     2941 atattttccg acaatggcag gattttctat agcaccataa attgccttga acttaaaagc
     3001 atcgttataa ctgaataaaa cactctccaa acggttatcc acatcaaatt ccaggtcttt
     3061 ataactgcga aaaacaatgc cgttgccaaa actttcttcc gttattccag cataaatttt
     3121 acaggcatct ttttcgtaag aggcatacaa ttcctgccag ccaagttcta aacggttggc
     3181 atccagttca tccaaaagtt ccgtttgttc agtggaatat ttaggcagtt cagcaataaa
     3241 cttcattcca aagctgaaat ttctataggc aagattgaaa ccgaaggtat cgttaaaata
     3301 ggtatgcaaa gagtcctcaa ccgtgcgata gatgaatttt gcctcgttgg aaccgtttac
     3361 agacagggcg ttttgagcac taagcaaagt aaagctgatt aacagcatta taaacagggt
     3421 atgtttcatt ttctgttcca ccatttatat ttgttgaact aataccagaa actctcacaa
     3481 aattttcact taatactata aattttgttc tgtctcccga gtaatattca ttcttccttc
     3541 agagtagtca agagtcctca ctgccaaatg aattcgtgaa aggtgtaaag gttatttcgg
     3601 cagtaacgag tcctgacttt tcctcatcaa ccaagttata atcatagatt aaaacctcaa
     3661 ataaaaaatc tgcgtaatct gcgtgcaact atccttattc cgcttcggtt tcaagtccca
     3721 ataaattacg gatatgcatt tcgtattcat tttccacacc aggttcatag ccaatatgag
     3781 aatagacaat ttctccagtt ttatttagaa taaaggtatg cggagggttt gttactccca
     3841 gttttttagc caaggttttt tcggaatcaa acagagttat aaacttataa ttcttgcttt
     3901 tcagatagtt ttttgccttg ggcagatttt taggagcatc aatagaaatc attactaccg
     3961 ttagcgaatc atacttttct gccagggtat ttaattgtgg catagcatct ttacaaggtt
     4021 tacagtaatc agcccaaaaa tcaatcagca caggaccctt tcccagaagt tcctgtagaa
     4081 taacattttt tcctgttgca tcgggaagct taaaatccgg catcatatct gccattgcaa
     4141 aagaaaccaa aagcagcagg agcaaacagg caatcggttt tttcattgtg aacctccttt
     4201 ttttacagca agaggatatt gtaagccact atatatagta gcgttattag caaaggtggc
     4261 aggtgttttc tgggtaaaaa tcactaatgc ggcatcctca ggaattgtca cattagcatc
     4321 aaattcaaag gcaacagggt tacttaaatc cacttcgctt atatcaatgc gtttcctgcc
     4381 tattaccaca ttggtaagat gctctccttc agcatttagc gctgtggaaa ccaaatcaat
     4441 aactgccaga ttcagaatca gttcttcctg attaaaggat gtggatagtg gagtaagttt
     4501 tacgcttccg gagatatttt ttccctctac agtagcgttt agaacactgt aatccatctg
     4561 gcttaataat tccaattcac tttgcacgat ataaggatat tggtttaaag tttcctgatt
     4621 tccacctacc agtttatgtt ttccttgaat aatagaaaca ggagcactgt atgcgctgta
     4681 ataagcatag agaggatcgt taaaagcaac cggaccggaa agctgatgtg ttaaatagat
     4741 gaagtcattt ccatattgag agcttaaatc gtgtaaaaga ttttccacag caggacagta
     4801 actgcaaccc acattagtaa catattccac tataactttt tgagttggag cagaaggatc
     4861 acagccacat tgattgcctc gcagcagcca ttttccgttt tctttcttta agcgttctcc
     4921 ggtatataag ctatctgcaa taacattttt acggctatca ctaatggtta aacgccagtt
     4981 tgccaaagca ttagtttcat tttgcagagt agctgttatt aaagttacat caataaaagg
     5041 attatttata ccggaaagca gattgtataa gatagcttcc cactcgcttt tagtaatacc
     5101 ataatgttta tagtcctctg cataaaaagc catcacagta gataaatctt ccgcgctggc
     5161 agtattaaat gcttcttcca aaggactgaa aagttccgca ttaaaatcca cctgttccag
     5221 tggcttaaaa ttatggtcaa agcgatcgca ggaaaatatg cctaaaagtg caactattaa
     5281 cagggcataa acaattttct ttttcatctt ttttcctaac tttatctatt ctgcttgggc
     5341 aggaatttta ttaagagcat ttattacctc atctttggaa agcagttcgc caaaaatatg
     5401 gggttctgca cccggaatat ataaagcatt aaaaggaacg cctgctcttc cgtgtttttt
     5461 tatccaagca agcaaggttt catctttttt ggtgaaatcc cctctcagca gcagaacatt
     5521 tttcttctta aattcctgca tcatcgtttc cgtaaacaga actgtctttt cattcgtcat
     5581 acaattttta caccatgcag ctccaatgtc cagaaaaacc gctctacctt ctttcagtgc
     5641 cttatttaaa atatcttcac taaacacata ccaaccctca ggagcattgg gagcaggaat
     5701 cagttcgcca ctttttacct gaatttcctc ttgtttcgct tctttaatcg gtagataagt
     5761 aacagcggca aagataatta acccaataga aataagcgta aaaagccatt gcgttaattt
     5821 gctgttttcc acccttacaa acctgccata cagccaggtg gaaaaaccaa gaataaacaa
     5881 aaaccacaga actttcatta gatattcacc atcggttaac agcagcaagg ttttcagctg
     5941 agtgtaaaca ataataagca aaacaaaacc catcacttcc ttgaaaataa tcatccagtt
     6001 gcctggcttg ggaattattt tcaacgcctt ggggaaaaag ccaatcagca gaaatggaaa
     6061 cgcaaaaccg agcccgatag tgagaaagaa aatcagcatt aaaacaggtg gtaaagccaa
     6121 ggcaaaaggc aaagctgcac ctaaaaaagg tcccgtgcag gatatcgcca tcaaaaaggc
     6181 aaaaataccc ccaaaaaagg aaccgctata gccccctcgg gaagttgcct tattagccgt
     6241 attcattccc ggaacagtta tttcaaagac ccctaatagt gacaaagcaa acacaaacac
     6301 aattgacatc agggttacca caaaaccagg attttgattt tgcaaacccc aacctaccga
     6361 ttcacctgcc ttttgcaaag cgatgaaaat tcctgccatt acggcaaagg aaatcagcac
     6421 ccccaaagcg taaatcaaag tatgattcag cactttggtt ttatctttct gtgcttgatt
     6481 cattatactc ataattctta tcggcaaaat gggtaaaacg caaggtgtta tatttaatat
     6541 aattccgcct aaaaaggcaa aaagaatgta ttttagtatc tcgccaagag gatggctggt
     6601 ttcagatttt ttatcgggaa tcggggattt ttcaatagga atggtttcct tttgttctat
     6661 agtgtttggg atttcctctt cattttccga aacagcagca ggtattggtt catttccaac
     6721 tattttaata gaaaggatag ctgttgcctc ttcgggtgga tcacacattc ctgtttcgta
     6781 gcatagatta tagctcagca gaacttcaat ctgcttgttg cctgttttag ctgtttgttt
     6841 caccgtaaaa ggcaaagtta aagttacttg gggatgatag ttccattcct cttccgaaac
     6901 aacctgtgtc ggtttcggat aaataacttt accccaaata aggtctggat ggcttgcttc
     6961 cagataaaaa tactccgggt tttggggatt aacggtttgt tttttccctt caggaataag
     7021 aagagtggct ttaataacac ctttttctcc aggcttcaag acctcaggag aaatagagaa
     7081 tttaacgctc tgtgcaaaaa ttggcagaat gagcagcact aaacagaaca ttaacaacag
     7141 cttaagacat ctatgcatca gcaatatcct tgttacatat ttgggtactt atagtaaatg
     7201 ataatgcaga aaacattcca gacaagacaa ctattgtggg aatgaaaaat aaagagtgta
     7261 tgaggggatt caaaaaaaat agcggattat aatgaaagaa aaatccaatt aatccagtat
     7321 atccagttat ctgcgttcta tatcttcacg ctcttaaaat ctcttttcct ctttgtaaaa
     7381 aaacccttta aacttccatt atttcttttt cttttgcctt ttccgcctca tctatctttt
     7441 taatccaatc atctgtaagg tcttgcagtt ctttcagttg cttcttttct tcgtcttcac
     7501 ttatttcgct gtcttttttt tcttttttaa ctatatcatt agcatcgcgg cggatattcc
     7561 ggatagcaac tctggtgtct tccgagattt ttttaatatt tttaactata tctctgcgtt
     7621 tttcttcggt taagggctga aaaggcaggc ggataacatt tccatcgttt tccggagtaa
     7681 tgccaatatt ggcagctaaa atggcttttt ctatatctgc cagggttgtt ttatcccatg
     7741 gctgaataac aatcattctg gcttcgggaa tggaaatgtt gcatagttgt ttaatcggtg
     7801 taggtagacc gtaataattg attttaatat catccaaaat ggaagcgctg gcgcgtccgg
     7861 ttctgatttt ggaatattga tgcagcattg catcaaagct cttttgcatt ttttctttag
     7921 tattatcttt tatattttcc ataatatcct ctttaccagg cagaaactat gttcaggggt
     7981 gaatataggt tcctgtttcc gcattagtaa gtgcttcaat taacatttgc ggctgactga
     8041 tgttgaaaat cttaattggc atagcattat ccatagccag agaaaaagcg gttaaatcca
     8101 taactcccaa gcgtttctgc aaacattcct caaagctggc actgcttata aaatgggcat
     8161 ttttatcttt tttgggatcg gcactgtaaa gtccatccac attagttgct tttaacacaa
     8221 tatcagcctg cagttcaaca gcgcgtaaaa cggcagcggt atctgtagta aaataaggat
     8281 ttcctgttcc tccgctgaaa aaacagacct ttccctgttc caaagcggag gaagccaatt
     8341 gaggagtata gcgatcaacc actttatcca cagccagtgt ggaaaaaatg gcacagtcaa
     8401 ttcctttgga aataaggatt tctgccatat acaaggaatt ctggatagtt gccagcatac
     8461 cgatactatc cagaactacc ctgtttaaat ttttattttt ccaggaaccg ccgcggaata
     8521 tatttccccc gcctaaaacg atgcctaatt cataattttg gttatgaact gtaattatgg
     8581 catcggtcag ggagtcaatt actgtttcat cgtagattat gcctttttta ccagaaagaa
     8641 cttctccgga aagtttcaac agcacccgat ggatattttc gcttttgtaa acatttttca
     8701 tacttgaact cccggctgtt tcaggaaggt ttacagcttt atgttcagct tttattcgcc
     8761 acctaattga taacggacaa atctggcaat ttgaatattt tcgcctgttg tagctatagc
     8821 gtttgtcagt aagtccttca cggtttttgt gctgtcgctg atcagttctt gacctaaaag
     8881 ggaacgctcg ttgcagaact ttttcaggtt accttccaca attttctcta tgatttcggg
     8941 ttttttacct tcattcacag ctttgttata ggcaatttct ttttctcttt ccagaatagc
     9001 gggatcaatt tgctcgggac ttatagctaa agggttggta gcggcaattt gcattgctat
     9061 ttcatctgcc aaagctttaa attcatcggt tctggcaaca aaatcggatt cgcaattcag
     9121 ttccagaagc actccaatcc ggttgttgaa atgtatgtat gaatgaatga taccttcttt
     9181 agtggcacgg tttgcttttc cttctgcttt actgattccc ttttcccgca ggtattttat
     9241 tgcctcatca atatttccgt ttttttcaat aagggctttg cggcaatcca tcattccggc
     9301 tccggttcta tcacgcaatt cttttacttg ggttgcagta atctctgcca ttttttctcc
     9361 tttttggtta aagtaagttt tggggttaat gttttattgt tccgtttccg ctaattcggg
     9421 aacatcaaat tccacttttt cttctgctgc agccaattcg gcttcaaaat cctctgtttc
     9481 ttcttcagca gttgtttcag ttgtttcttc ttcagtgctt tctccctctg tggcaagtcc
     9541 ttttccttca ataacggcat ttgccataat atctgaaatc agatgaatgg cacgcgtggc
     9601 atcgtcgtta ctgggaatca cataatcaac caaatcggga tcgcaattgg tatcaaccat
     9661 tgccacaatc ggaatattca agatacgtgc ttcgtgcaca gctatttttt catattcagt
     9721 atccacaata aaaacacatc ctggcagggc atccatttcg cggataccgc ctaaggaaaa
     9781 ttcaatttta tcgtgcattc ttttcatttt ttgttgttcc agcttggtgt aaccattgat
     9841 agtgccgtca gccacgattt cttcataata cttcatcttt tcaatgcttt gacggatagt
     9901 agccatatta gttagcattc caccatacca acgctggttt acataaaaga caccggcttt
     9961 ttcagcagct tctttaatag ctgcctgtgc ctgttttttg gttcctacaa aaaggatata
    10021 ttcacctttg gaggcaacct ctttcataaa ctgatatgct tcattgatag catctactgt
    10081 ctgtttcaaa tcaataatat gaatcccgtt gcgtttaatg aagatgtatt tcttcatttt
    10141 aggattccat ttgaaggttt gatgcccaaa atggacaccc gcttcaagta attgtttcat
    10201 agttactacg gacattcttt tctcctgaaa tacggttttt aggtctcttc cggacggtgt
    10261 aagcaattca aaggaacaaa aacctcaccg ccttgcaagc gttccggaag tttatttaat
    10321 gttttttcca ttttgataat ccttttgtca ttcctgtgaa agcaggaacc agatgatttt
    10381 tcatttcttt aactgaacta tcaatatgga acttataaaa cttgttgttt ttcattccag
    10441 aaaaactgtt tcttttaacg cttagagaac tggaatctct ttctggcttt gggacgaccg
    10501 gattttttgc gttccaccat tctgggatca cgcgttaaaa atccccgggt cttcagcacg
    10561 ggacgcagat tttcatcata ttccactaag gcacgggaaa taccatgacg gatagctcct
    10621 gcctgaccgc ttaaaccacc tccgtaaaca tttacataaa catcaaagtt atcggataag
    10681 ccaacggtct gtaagggctg ttctacaatc atttccaggg tctcacgctg gagatatttc
    10741 ttcatctgga tattattaat aatgcgtttt ccagttccag gcataagacg aactctggca
    10801 acggcatttt ttcttctgcc aactgcatca aaggtctgca tactttttta ctcctaaata
    10861 tttagttcaa tgggtttttg agcagaatgc ggatgttcag ctccaggata aactttcaat
    10921 tttttgaaca ttgcgcgtcc taacttattt ttgggcatca taccttttac ggcatgttca
    10981 ataatgcgtt cgggatgctc ttccaaaact ttggtataag gaatttcctt caaaccgtca
    11041 ggataaccac taaagctttt ataaaccttc tgtaatgcct tcatacccgt tacgcgaact
    11101 tttgctgcat tgatcacaat cacataatcg ccggtatcaa tattgggcac ataatacggt
    11161 ttatgtttac cacataaaat tgtggcaact tttgtggata agcgtcccaa aggttttccc
    11221 tctgcatcta cgatatacca ttgctggtga atatcggaag gactaggggt tagggtcttc
    11281 atcgcttctc ctttacatat ttttttattc agaaagtcac atttttggaa gcaggaatag
    11341 tgtcaagtgt tttgaagggt ttaaacaatt ggaggcataa agtgaaaaaa tggaaagctg
    11401 aaaagggaaa caagggaata attttgggga caataataag attagagaca gggaaaaagt
    11461 caagaacaaa
//
LOCUS       DAOOZV010000057        11402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5586_length_11402_cov_63.421080, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000057 DAOOZV010000000
VERSION     DAOOZV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5586_length_11402_cov_63.421080"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1462)
                     /locus_tag="PLW08_06305"
     CDS             complement(158..1462)
                     /locus_tag="PLW08_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPL52247.1"
                     /translation="MNSAIFPDNLKELSFALRQGKLPLQDYLFQTLADIEKKEPVLCA
                     LEPEENRQERLLKQAEELLSRFSQPELRPPLFGVLIGVKDLFNVDGLPTRAGSKLPPE
                     VFSGKEAEIVTRLKNLGALILGKTACTEFAYFQPAKTKNPCNPNHTPGGSSSGSAAAV
                     AAGYCLLALGTQTIGSIIRPASYCGVTGLKPSYGLFSLQGVFPFSQTVDHLGFITKQV
                     ADLAFLLPLLHLSVSTKITDRPVLGAVNGSYLQQADSSVQENYCSALDKLQKKGYKIV
                     TCDPFGDINSINASHNALIAAEFSLNHKSLYERYQELYSDSSKELYARGLQISSAELE
                     NLRQQTIILREKIINLMQEQKIDLWITPSTTTTAPAGLKSTGSPLMSLPWTNCGLPAL
                     TIPNGLDANGLPFGLQFVASYNEDLPLVRMVQRIVNTQEIET"
     gene            complement(1508..2698)
                     /locus_tag="PLW08_06310"
     CDS             complement(1508..2698)
                     /locus_tag="PLW08_06310"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPL52248.1"
                     /translation="MNKPLQDVVVLDLSRVLAGPFCTMILSDLGAEIIKVEHPEKGDD
                     SRAYGPFLHNRSLYFLSINHGKKSISLNLKKPQGRELLLKLIEHCDVLVENFRPGTME
                     KFNLGYEFLKEKNPQLIYAAVSGFGHSGPDSQKPAYDILVQARGGIMSITGWQNMAPT
                     RVGMSLGDITASLFTAIGINSALYQRTKTGLGQKIDVAMLDGQIAILENALVRYQAEG
                     ISPKPLGNRHPTISPFQAYKASDNYFVIAVGNDNLWNTFCKALEIPELISDKRFCNNR
                     LRTENIDALNAILEPIFTAKPASWWLELMEKSGIPCSSINNIESVMQDPQLLARNMFV
                     EVNDPIAGTVKIAGNPIKMSSFPDSAVYEPAPEIGAHNKEIYCERLGLSEEDLSELQK
                     VGVI"
     gene            complement(2695..5034)
                     /locus_tag="PLW08_06315"
     CDS             complement(2695..5034)
                     /locus_tag="PLW08_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPL52249.1"
                     /translation="MKGYKYIGKDVTRIDALDKISGAATFVDDIDFGANLLYAELIES
                     TQASALIKNIDVSEALKVKGVYKIFTGKDFHNRFGLYMKDRYVFAQERVRFVGEQIGA
                     VVAFTPEAAKSAVSLVKVEYEPLPAVLNQMDALKSDAPLLHPEVENYPRVPWFYPKGG
                     TNIAHWRKTRKGDLEKGFANADVILEETYSVPRYAHCALETHIAVAKFDHSGRLTVWS
                     SSQSPHTQRNLFAEALGLSHKDVRVITPYVGGGFGGKAGVTMEIIPAVLATQLKGYAV
                     KLRYSREQEFTNTYQRLGLITKIKMGATKEGIITALEHKLYWDAGAYVEYGANVVNAV
                     GLSASGPYRIDNVSIDSICVYTNLPPGGPYRGFGYSEMLFGLESHISRMAHQLNMDEV
                     EFRRINAIKPGDKTAYGAKMNPSGLQECIDKAAKAIDWGKKPISEDPTKVIGQGFAIF
                     WKAPAMPPNASSSAFLKFNEDATINLLISGMDIGQGYQTVMAQIAGEILSVPPAKIRV
                     ETPDTDRNPYEWQTVASHITWSCGNAVKNAALDAREQIFTLIHRVYHFPLDALYLEDE
                     AVKCKTDPAFILPLKNFVVEGIMTEDGTFKGGPINGRGMFMPEFASTKDNPETSQGGH
                     PNVHYTVGAAGLNLEIDQETGKMKVLKVALAIDAGKAINPDLVKGQITGGLLQGLATV
                     LYEDMRFNEKGKLLNPNLTDYKIPTAKDVPEEIIPIIVEVPQPDGPFGARGIGEHTML
                     PAAPMIANAIEDALGIRIKSLPITAEKVALTCLREKAKQ"
     gene            complement(5031..5495)
                     /locus_tag="PLW08_06320"
     CDS             complement(5031..5495)
                     /locus_tag="PLW08_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPL52250.1"
                     /translation="MKEISLILNNEPRNVQVETNEILLDVLRNKLGIKSPKCGCERGD
                     CGACTVLLDGKAVRSCLILAVEAENHSITTLEGISREKLSALQEAFIEFNSFQCGYCA
                     PGIILAVTELLEKNPHPSLEEIKESIAGNLCRCTGYTPIFDAIMSLTGKGAK"
     gene            complement(5495..6433)
                     /locus_tag="PLW08_06325"
     CDS             complement(5495..6433)
                     /locus_tag="PLW08_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPL52251.1"
                     /translation="MSHYGLGTLKKEENMALAHTFDYQRPSTLIEALQLKAKYKDKAL
                     FLAGGTDLIVNIREGMLKPDLLIDIKGISELLKLNWEKETLFIGSNVTFAQLIENELV
                     QKHFILLHDACKTIGSTGIRARATLTGNICSAVPSLDSAPALLCYEAKVHCASPESQR
                     EIPVTEWFLAPRKTALQEDEIVTGISLKRIPDSSGIYLKLGRYSGEDLAQAGWGILMT
                     ADKQYRLAHCALSPIPKRAYYIEDLLNNSNPLNADLIEKAIALIPSEINPITDIRATQ
                     EFRMYISGIMLKRGLTAIQERISGKYIEPRKLLGDV"
     gene            complement(6406..7218)
                     /locus_tag="PLW08_06330"
     CDS             complement(6406..7218)
                     /locus_tag="PLW08_06330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPL52252.1"
                     /translation="MELNSFFEFMQSLKMYDLTQPLSIHTPPWPSYMPLGIQYFKRIA
                     GAHSGQGANGQIITTSNHVGTHIDGEIHFFASGRSIGQVPMEEWIGPGVVVDISDSVN
                     DYDLYSPELLMQKAEIKKGDILIINTGYHRYAWDQPESDELRYFVKHPGPDPSFHTWA
                     LDMHLKWIGVDCGSADHPMNTIIRNWHPGSFKEAEQKLIAKYSKTWDEMFPPEVYYQV
                     MHLKLFPKKLVHAENLGGDIDKISNKRVWIGLFPLRGIELESSMCRIMAWEP"
     gene            complement(7397..8794)
                     /locus_tag="PLW08_06335"
     CDS             complement(7397..8794)
                     /locus_tag="PLW08_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPL52253.1"
                     /translation="MLKLKKDKPKVINIGLEKFAIDLKTQGIDVVNLLWKPPLPVENS
                     YHHKINSLSENIIAPANKKALEIINSGKAVLTGMDVALKVIPGMHKDLILHSGPPISW
                     DRMCGPMRGAIIGALIYEDRAKNAEEAEKLAASGKIEFAPCHEHNCTGPMAGIISPSM
                     PVFIIKNESYGNFTYATQNEGLGKVLRYGAYSEEVISRLKWMEKVLYPVLKRAIEYAG
                     TINIQGLIAQALNMGDELHNRNRAGTSLFIRQIAPAIIKTMTNPEESAAALDFINSND
                     HFFLNLSMPASKAILDPARNISGSSIVVAMARNGTDFGIQVSGTGNKWFTGSAPVPDA
                     LYFPGFTKEDANPDIGDSSITETGGLGGFALAAAPAIVQFVGGTAKEAINYTLSMYNI
                     CFGESSLYQIPALDFRGTPLGIDVVKVIQNNIAPVIDTGVAHKNPGVGQVGAGVVKAP
                     FEPFIKAYKTLADMY"
     gene            complement(8788..10305)
                     /gene="fdrA"
                     /locus_tag="PLW08_06340"
     CDS             complement(8788..10305)
                     /gene="fdrA"
                     /locus_tag="PLW08_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HPL52254.1"
                     /translation="MPIKAKIIAGKYLDSVKLMLISRELRSLKGVLEAVAIVATSENR
                     EILKASDMLAEEINSASENDIVIVIKADNAKNAAEAINKAEELINKPSANEYNQTPIP
                     QAFTLEKAIATLGGADLCLISIAGKYAAAEAEKALEKGLHILLFSDNVSIEDELSLKQ
                     KALKKGLLMMGPDCGTAILNGVPLAFANNVPRGKIGIVSASGTGLQEISSTIANLGGG
                     ISQAFGTGGRDGKKEIGGLMLTACLEYLLEDENTEVIIVTGKPPCEEVQKKLWELISH
                     SSKPVIANFLIPMQVPELPNLYYVRSLSETAFFACKKAGIEVKTAKLAEDFPLPKLRG
                     PYLRGLYSGGTLCQEAIQVYLDNFGCEPFNNVTDQAFFRLTDGWQTKENSIIDLGADE
                     FTIGRPHPMIDYSLRLQKLAQEANDSKVGIILLDVVLGFGAHPNPAEELVPALQKLPA
                     DLVVVCHLLGTDGDPQNRQKQKKELEQVGAYVFTSHHSACEFVCQCLRSYRSAKC"
     gene            complement(10373..11230)
                     /locus_tag="PLW08_06345"
     CDS             complement(10373..11230)
                     /locus_tag="PLW08_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPL52255.1"
                     /translation="MQRKNNYTLFRIGELVPSGTYTKVHSCFERVINFAAEDIVISLC
                     ESSICAGPYRLILKGKIPLAKELKVTEKEVVINQEISLPYSQDNIWKIPSIHPYKNAQ
                     KTYFLCEKLLNFFMGEEKNNLLSLLFQPPSSQDEFLLQFRNFLIKGINNLFQQKYTLF
                     AQQIKRRGLGSTPAGDDFLIGYIICLSWLIANGRKDLIKQREEIYQTALDNDLLVNTF
                     LHQAYYLRPDDDWANFLLALSENDEINLTKYINLIMNSGESSGKDMLSGFVFCLLFNW
                     FWIAEIAKC"
BASE COUNT         3387 a         2467 c         2106 g         3442 t
ORIGIN      
        1 caaattggaa gttgccaatc tattagccct caattgcatt attcggtaga gaaaattgtc
       61 catcattttt cagagttccg caaactcttt ggcaaattgg aagttgtcac ccccttttag
      121 ttcaatcgca ttattcggat gcaaaatcct gcaaccttta agtctctatt tcctgtgtat
      181 taacaatcct ttgaaccatc ctaaccaatg gcagatcttc attataggaa gccacaaact
      241 gcaagccgaa aggaagacca ttggcgtcta agccattggg aatagttaaa gccggcaaac
      301 cgcaattcgt ccagggcaaa ctcattaaag gtgaaccggt tgactttaat cccgcaggag
      361 cagtagttgt ggtagaagga gtaatccaga ggtcaatttt ttgttcttgc atcagattta
      421 ttattttttc tctaagtata attgtctgtt ggcgtaaatt ttccagttcc gcggaactga
      481 tttgtaatcc tctggcatac aattccttag aggaatcgga ataaagttcc tgatagcgct
      541 catataaact tttatgattt aaggagaatt ctgccgcaat cagggcatta tggctcgcat
      601 taatagagtt tatatcgcca aaaggatcgc aggtaactat tttgtatccc tttttctgta
      661 gtttatcaag agcactgcaa taattttcct gaacggaaga atccgcctgt tgtaaataac
      721 ttccgttaac tgcacccaaa acaggtctgt ctgttatttt ggtagaaacg gacagatgca
      781 aaagagggag taaaaaagca aggtctgcta cttgttttgt gatgaaaccg agatgatcaa
      841 ctgtttgaga aaaaggaaaa accccttgca gagaaaacaa accataacta ggttttaaac
      901 ctgtaactcc acagtaagaa gcaggcctga taatagagcc aatggtctga gttcccaaag
      961 ctaaaagaca atatcctgca gctacagctg ccgctgatcc actggaagaa ccacccggag
     1021 tatgattcgg attacaggga ttttttgtct ttgccggctg aaaataggca aattcagtgc
     1081 aggcggtttt ccctaaaatt aatgctccca aattttttaa gcgggtaaca atttccgctt
     1141 ctttgcccga aaaaacctct ggaggaagtt ttgaacccgc acgtgtaggt aaaccatcca
     1201 cattaaacaa atctttaacg ccaattaaaa ccccaaaaag aggtggacgc aattccggtt
     1261 gagaaaaacg ggataacagt tcttcagctt gtttcagtaa gcgttcttgc ctgttttctt
     1321 caggttccag agcacataaa acgggttcct ttttttcaat atcggcaagg gtctgaaaaa
     1381 gataatcctg caaaggaagt tttccctgcc gtaaagcaaa agaaagttct ttcaggttat
     1441 cgggaaaaat agcggaattc atctaatatc cttccggatt ttaaggaacc acaagaaaat
     1501 attgatgtta aatcactccc accttttgca attcggataa gtcctcttca cttaagccaa
     1561 gtcgttcaca ataaatctct ttattgtgtg ctcctatttc cggagcgggc tcatacactg
     1621 ctgaatccgg aaaagaactc atttttatag gatttccggc aatttttaca gtgcccgcta
     1681 tagggtcatt aacttccaca aacatattac gggcaagcaa ttgagggtct tgcataacgg
     1741 attcaatatt gttgatagaa gaacagggaa ttccggattt ttccattagt tccagccacc
     1801 aggaagcagg tttcgccgta aaaataggtt ctaaaatagc atttaaggca tctatatttt
     1861 ccgtgcgtaa gcgattattg caaaaccgct tatccgaaat taattccgga atttccagag
     1921 ctttgcaaaa ggtattccaa aggttatcat tccccacggc aatcacaaaa taattatcgc
     1981 ttgccttata tgcctggaat ggagatattg taggatgacg attgccaaga ggtttgggtg
     2041 aaatcccttc tgcctgataa cgcactaagg cattttccag aatggctatt tgaccatcca
     2101 acatagcaac atcaattttt tgtcccaaac ctgtttttgt gcgttggtat aaagcagaat
     2161 taatcccgat ggcagtgaaa agcgaggcag ttatatctcc aagagacatt cctacacgtg
     2221 taggagccat attttgccaa cctgtgatac tcataatacc gcccctggct tgaactaaaa
     2281 tatcgtaggc aggtttttgg gaatcaggtc cgctatgccc gaaaccggaa acagcagcat
     2341 aaatcaattg cggatttttt tcctttaaaa actcatatcc caggttaaat ttttccatcg
     2401 tgccagggcg gaaattttct accaaaacat cacaatgttc tatcagcttt aaaagcaatt
     2461 ctctcccctg cggttttttc aggtttaacg agatactttt cttcccgtgg ttgatactga
     2521 gaaaatagag agaacgattg tgcagaaaag gaccataagc tctggagtca tcgccctttt
     2581 caggatgttc caccttgatt atttctgcgc ccaaatcgct taaaatcatt gtgcaaaaag
     2641 gacctgccag aacacgacta aggtcaagaa ctacaacatc ttgaagcggt ttattcattg
     2701 ttttgccttt tcccgcaaac aagttaatgc cactttctct gccgttatag gtagggactt
     2761 aattctaatg ccgagagcat cttcaatggc attggcaatc attggagctg caggaagcat
     2821 tgtatgttct ccaattcccc gcgctccaaa aggaccatcc ggttgaggaa cctctacaat
     2881 aatcggaata atctcttccg gaacatcttt agcagtagga attttatagt ccgtaagatt
     2941 aggattgagc agtttacctt tttcgttaaa acgcatatct tcatataaca cagttgctaa
     3001 accctgaagc agacctcccg taatttgccc ttttaccaaa tcaggattga tggcttttcc
     3061 ggcatcaatg gctaaagcaa ccttcagcac tttcattttg cccgtttcct gatcaatttc
     3121 caaattcaaa cctgccgcac ctaccgtgta atgaacatta ggatgacctc cctgacttgt
     3181 ttccggattg tctttagtgc tggcaaattc aggcataaac attccgcgac cgtttatagg
     3241 accaccctta aaagttccgt cttccgtcat aatgccctct accacaaaat ttttcaaagg
     3301 taaaatgaaa gcgggatcgg ttttgcattt aactgcctca tcttccagat aaagagcatc
     3361 cagcggaaaa tgataaaccc tgtgaatgag cgtaaaaatc tgctcccggg catccaatgc
     3421 ggcattttta acagcatttc cacagctcca ggttatatga cttgcaacag tttgccactc
     3481 ataaggattc cgatccgtat caggtgtttc tactctaatt ttggctggtg gaacagataa
     3541 aatttctcct gcaatctgag ccataactgt ctgataacct tgtccgatat ccataccgga
     3601 aataagcaga ttgattgtag catcttcatt gaactttaaa aatgcggaag aagaagcatt
     3661 cggaggcata gcaggcgctt tccaaaaaat agcaaagccc tgaccgataa cttttgtcgg
     3721 gtcttcggaa atgggttttt tgccccaatc aattgctttt gcggctttat caatacattc
     3781 ctgtaaaccg ctcgggttca ttttagcacc ataggcggtt ttatctcctg gcttaatggc
     3841 atttatcctt ctaaattcta cttcgtccat attaagttga tgagccattc tactgatatg
     3901 actttccaag ccaaaaagca tttccgaata gccaaaaccg cgataaggac cccccggagg
     3961 aagattggta taaacacaaa tagaatctat ggaaacatta tcaatccgat aaggtccgct
     4021 tgctgataaa ccaactgcat taacaacatt tgctccatat tctacataag cacctgcatc
     4081 ccaatataat ttatgttcca aagccgttat aattccctct tttgtagcac ccatttttat
     4141 ttttgtgatc aatcccaatc gctgataagt attggtaaat tcctgttccc tgctgtaacg
     4201 cagcttaact gcataaccct ttagttgagt ggctaaaact gcaggaataa tttccatcgt
     4261 aactccagct tttcctccaa aaccacctcc cacataagga gttataactc tcacatcttt
     4321 atggctgagg cctaatgctt cggcaaaaag atttctttga gtatgcggag attgtgaact
     4381 gctccaaacg gttaatcgcc ccgaatggtc aaatttggca accgctatat gggtttccaa
     4441 agcacaatgt gcataacggg gaacggaata tgtctcctcc agaataacat ctgcgttggc
     4501 aaaacctttt tccaaatcac ctttgcgggt ttttcgccaa tgagctatat ttgttccgcc
     4561 tttgggataa aaccacggta cgcgaggata attttctacc tccggatgca gtaaaggagc
     4621 atcacttttc aaagcatcca tttgatttaa aactgccgga aggggttcat attcaacttt
     4681 aaccaaagaa acagcacttt ttgcagcttc cggagtgaaa gccactaccg cacctatttg
     4741 ttcgcctaca aaacggactc tttcctgagc gaaaacataa cggtctttca tataaagtcc
     4801 gaaacgattg tgaaaatcct tccctgtaaa tattttataa acgcctttca ccttcaatgc
     4861 ttcggaaaca tctatattct tgatcaaggc agaagcttgc gtgctttcaa tcaattctgc
     4921 ataaagcaga ttagctccaa aatcaatatc atcaacaaaa gtagctgctc cgctaatttt
     4981 atccaaagca tcaatacgcg ttacatcttt gcctatatac ttatagccct tcattttgcc
     5041 ccctttccgg ttaaagacat aatagcatca aaaataggtg tataacctgt acaacgacaa
     5101 aggtttccgg caattgactc tttaatttcc tctaaagagg gatggggatt tttttccagc
     5161 aattcagtaa ccgccaaaat tattcccggt gcacaataac cacattgaaa agaattgaat
     5221 tcaataaatg cttcctgcag agcactaagt ttttccctgg aaataccttc taaagtggta
     5281 atactatgat tttcagcttc aacggcaaga atcagacaac tgcgaactgc tttaccatca
     5341 agcaaaacag tgcaggcacc gcaatcacct ctttcacagc cacatttggg acttttaatt
     5401 cccaatttat tccggagcac atccagaagt atttcattag tttccacttg cacattacgg
     5461 ggttcattat taagaatcaa gctgatttct ttcatcatac atctcccaaa agctttcggg
     5521 gctcaatata tttgccggaa attctttcct gaattgccgt taaacctctt ttaagcataa
     5581 ttccactaat atacatccgg aattcttgtg tagcgcgaat atctgttatg gggtttattt
     5641 ccgaaggaat aagagctatg gctttttcta ttaaatcagc attcagaggg ttactgttat
     5701 ttaagaggtc ttcaatatag taagctcttt tgggaatagg acttaaagca caatgagcca
     5761 aacgatattg tttatccgca gtcattaaaa ttccccaacc tgcctgagca aggtcttctc
     5821 cactgtaacg acccagcttt aaataaatac cggaactatc aggaattctt ttcagagaaa
     5881 taccggttac tatttcatct tcctgcaaag ctgtttttcg gggagcaaga aaccattccg
     5941 taacaggaat ttccctttgt gactcaggac ttgcacaatg aacttttgcc tcatagcata
     6001 aaagagcagg agcagaatcc aaagatggaa ctgcagaaca aatatttccc gttaaagtag
     6061 ccctggcacg aattcctgta gagccaattg ttttacaggc atcgtgaaga agaataaaat
     6121 gcttctgcac cagttcattt tctataagct gcgcaaaagt tacattactg ccgataaaaa
     6181 gtgtttcctt ttcccaattg agctttaata attcactgat accttttata tcaatcagca
     6241 aatcaggttt cagcattcct tctctaatat ttacaatcaa atcggttcca cctgccagaa
     6301 aaagggcttt atctttatat tttgctttaa gttgcaatgc ttcaatcaaa gtggaaggac
     6361 gctgatagtc aaaagtatga gccaaagcca tattttcttc cttttttagg gttcccaagc
     6421 cataatgcga cacatagagg attccagttc aatgccgcgc agaggaaaaa gtcctatcca
     6481 gaccctttta ttggagattt tatcaatatc tccccccagg ttttctgcat gcaccagctt
     6541 tttagggaag agtttcagat gcatcacctg atagtatact tcaggaggaa acatttcatc
     6601 ccaggtctta ctgtatttgg caatcaattt ttgttccgct tccttaaaag aaccaggatg
     6661 ccaattgcgg ataatagtat tcataggatg atctgcagaa ccgcaatcta ctccaatcca
     6721 tttcaaatgc atatccaaag cccaggtatg aaaagaagga tcaggacctg gatgcttaac
     6781 aaaatagcgg agttcatcac tttcaggttg atcccaggca taacgatgat agccggtatt
     6841 gataattaaa atatcgcctt ttttgatttc cgctttttgc atcagaagct cgggagagta
     6901 taaatcatag tcatttacgc tatcagaaat atctacaact actccaggac caatccattc
     6961 ttccatagga acttgtccta tactgcgtcc gctggcaaaa aaatggattt caccatcaat
     7021 atgtgtgcct acatgattgc tggtagtaat aatttgacca tttgcaccct gacctgaatg
     7081 agctccggca atgcgtttaa agtattgaat tcccaaaggc atatagctgg gccagggagg
     7141 tgtatgaata cttaagggtt gagttaaatc atacatcttt aagctctgca taaattcaaa
     7201 gaagctgttt aattccattt ttatctcctg tttatatagt tcaaagttca aagtgcggga
     7261 gttcaaagtg cgggagttca aagtgcggga gttcaaagtt cattataaca aagatatcgt
     7321 ttttccggaa aaccatctcg ctattttcac catctcacta ttttcccatc tcactatctc
     7381 actctttatc ttttttttaa tacatatccg ctaatgtttt ataggcctta ataaacggtt
     7441 caaaaggtgc tttcacaact ccagcgccaa cttgtcccac accaggattt ttatgtgcca
     7501 cacctgtgtc aattaccgga gctatgttat tttgaataac tttcactaca tcaattccca
     7561 aaggggttcc tctaaaatcc aatgccggaa tttgataaag ggagctttct ccaaaacaaa
     7621 tattatacat agacaatgtg taatttattg cttctttggc agttccacct acaaattgaa
     7681 ctatggcagg agctgcggca agagcaaaac cacccagacc gcctgtttct gttatagagc
     7741 tgtcaccaat atcgggattg gcatcttctt tggtaaaacc tggaaaatag agggcatcgg
     7801 gaacaggggc actacccgta aaccatttat ttcctgttcc gctaacctga attccgaagt
     7861 cagttccatt tcttgccata gccacaacaa tagagctacc ggaaatgttt ctggcaggat
     7921 caagaatggc tttacttgca ggcatagaca ggttcaggaa aaaatggtcg ttgctattta
     7981 taaaatccag tgcggcagca gattcttcag gattagtcat tgttttaata atggcaggag
     8041 ctatttgtct gataaacaaa gaagttccag cccgattacg attgtgaagt tcatcaccca
     8101 tattcaatgc ttgggcaata agtccctgaa tattaatcgt tcccgcatat tctatagctc
     8161 ttttcaggac gggatagagc actttttcca tccattttaa gcggctaatc acttcttcgc
     8221 tataggcacc atagcgcaaa acttttccca aaccttcatt ttgggtagca taagtaaaat
     8281 tgccgtagct ttcattttta ataataaaaa caggcattga aggactgata atacctgcca
     8341 taggaccggt acaattatgt tcgtggcagg gagcaaattc aatttttccc gaagcggcaa
     8401 gtttttccgc ttcttctgca ttttttgccc tatcttcata aattagagct ccaattatgg
     8461 caccccgcat aggaccgcac attctatccc aggaaatagg aggaccgcta tggagaataa
     8521 ggtctttatg cattccggga ataaccttca gggcaacatc cattccggtt aaaaccgctt
     8581 tgccggaatt gattatttcc agagcttttt tattggctgg agcaatgatg ttttcagaga
     8641 gcgaatttat tttatgatga tagctgtttt caacgggcag aggaggtttc caaaggaggt
     8701 tgaccacatc aataccttga gttttaaggt ctatggcaaa tttttccaag ccaatgttta
     8761 ttacttttgg tttatctttt tttaatttca gcatttagca ctccgatatg atcttaaaca
     8821 ttggcagaca aattcacaag cagaatgatg agaggtaaat acataagctc ctacctgttc
     8881 caattccttt ttttgttttt gccggttttg aggatcgccg tctgttccta ataaatggca
     8941 gacaacaaca aggtctgcag gcaatttttg cagggcagga acaagttctt cagccggatt
     9001 aggatgagca ccaaaaccca aaactacatc caataagatg atacctactt tagaatcatt
     9061 tgcttcctga gccaattttt gcagacgcaa actgtaatct atcatcggat gaggacgtcc
     9121 tattgtaaat tcatctgctc ctaaatctat aatactattt tcttttgttt gccagccatc
     9181 ggtcaaccgg aaaaatgcct gatccgtaac attattaaag ggttcgcaac caaaattatc
     9241 aagatagacc tgaattgctt cctgacaaag cgttccgcca ctatataaac cgcgcagata
     9301 aggacctctt agctttggca aaggaaaatc ttccgcaagc ttagcagttt ttacttcaat
     9361 tcctgctttc ttacaggcaa aaaaagcggt ttcagacaaa gaacggacat aatataaatt
     9421 gggcagttca ggaacctgca ttggaataag gaaattggca atcaccggtt tagaactatg
     9481 agaaatcagt tcccacaatt ttttctgcac ctcttcacag gggggttttc ctgttacaat
     9541 aataacttct gtattttcat cctccagcag atattccaaa caggcagtca gcattaaacc
     9601 gccaatttct tttttcccat cccttcctcc ggttccaaat gcctgcgaaa ttcctccacc
     9661 caaattagct atggtagagg agatttcctg taaacctgtt cctgatgcgg aaacaattcc
     9721 tattttaccg cggggaacat tattggcaaa ggcaagtgga acgccattga gaatagcggt
     9781 tccgcaatca ggacccatca tcaaaagacc ttttttcaga gctttttgtt tcaaagaaag
     9841 ttcatcttca atgcttacat tatcggaaaa gagcaaaata tgcagtcctt tttccagtgc
     9901 tttttccgct tcagcggctg catattttcc ggcaatggaa attaggcaaa ggtctgctcc
     9961 tcctaaagtt gcaattgctt tttccagagt aaatgcctgg ggaattggag tttgattgta
    10021 ttcattagcg gaaggtttat tgattaattc ttctgcttta tttattgctt ccgcagcatt
    10081 ttttgcgtta tcggctttga ttacgataac aatatcgttt tccgaagcag agtttatttc
    10141 ttctgccagc atatcacttg cctttagaat ttcccgattt tcgcttgtag ctacaatagc
    10201 aactgcttcc agaacgcctt ttagagacct tagttcccgg gaaataagca tcagcttaac
    10261 agaatccaag tattttcccg caataatttt agcttttatg ggcattatat ttcctttata
    10321 tatttacaga tagcacattg agagcagcaa ggacatccgg aaaaatacaa cttcagcatt
    10381 tcgctatttc cgcaatccaa aaccaattaa ataacaaaca aaatacaaat ccgctcagca
    10441 tatcttttcc cgaactctca cctgaattca ttattagatt tatgtatttt gtcaagttaa
    10501 tttcgtcgtt ttcacttaaa gcaagcaaaa aattcgccca atcgtcatcc ggacgcaggt
    10561 aatatgcctg atgcagaaaa gtattgacca gcaaatcatt gtccaaagcg gtttgataga
    10621 tttcttcccg ttgttttatc aggtcttttc taccgttggc aatcagccag gataaacaga
    10681 ttatatagcc aatcaaaaaa tcatcaccgg caggagtgct tcccaaaccc cggcgtttta
    10741 tttgttgggc aaagagtgta tatttttgtt gaaagaggtt atttatacct tttatgagga
    10801 agttacggaa ctgaagaaga aattcatcct gggatgaagg gggctgaaaa agcaaagata
    10861 ataaattatt tttctcttca cccataaaga aattaagcag tttttcgcag aggaagtagg
    10921 tcttttgggc atttttataa ggatgaattg aaggtatttt ccagatatta tcctggctgt
    10981 atggtaaaga tatttcttga ttaataacca cttctttttc tgttactttc agttctttag
    11041 ctaaaggaat ctttcctttt aggattagac gatatggacc tgcacatata ctactctcac
    11101 ataaagagat aacaatatct tctgcggcaa aatttattac ccgctcaaaa cagctatgaa
    11161 cctttgtata ggtgcctgaa ggaaccaatt ctccgatgcg gaaaagggta taattatttt
    11221 ttctctgcat aacaataaaa taatgctttt gtaattaaat gtcaaatggg tagatggaga
    11281 acgctatttc tttgtaagaa aaattgcggg gctacagcga gctccggtta caaaaatcgt
    11341 gtaataaaaa atactcgcaa atcaataaaa tccgtgcaat ctgtgtaatc tgtgagagcc
    11401 gt
//
LOCUS       DAOOZV010000058        11336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5626_length_11336_cov_56.495346, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000058 DAOOZV010000000
VERSION     DAOOZV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_11336_cov_56.495346"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..2028)
                     /locus_tag="PLW08_06350"
     CDS             complement(172..2028)
                     /locus_tag="PLW08_06350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL52256.1"
                     /translation="MPVKKVIIYLVIILLIVEIIFAFPFFKNLEYKAQDMLFRMRGKL
                     PVSDKIVIIGIDDATFSALNETWPFPRDYHSKLIENLNKAGAKQIIFDIEFTEKTLPE
                     SDLRLAETANRYQNVIFAGKVLTSQRPGEPSQIIPPISPITEYNLPWGIVNMEADPDG
                     FIRKYTLFENFDQTKLYSIGIASLANLRVYQNNWAEHIKVNSKALHLTDKSIPLADKN
                     KAIINFRGPAKTYPAFSYASILDDSTFFMPGYQGAELDEFYEIRDSGVLKDKIVLVGA
                     TIDELHDKFPTPYGGEWTSGVEIQANFLDMVLTGNYLKKLNPWLFLLIEVVLLIGLWF
                     LFKVLKPQFSALALLGLLIILFIINLLLFNKAGIICPIAQTIILLILIYVASILNHYW
                     VTMKEKRFIRNAFQQYLAPELVNQLLANPKSLKFGGSLQEITVLFSDIRNFTTYSETH
                     SPQETVNILHEYLTEMVKIIINNHGILDKFVGDEIMALYGTPVPLPNHSFDACKTALQ
                     MRECLSRLQEKWQSEGIEPFEIGIGINTGMAVVGNLGSEQIFDYTAIGDTINLGARLE
                     GLNKEYNTFNHIIISEFTYEKVKDLVNANYLDEVKVKGKNIAVKIYELVSLK"
     gene            complement(2114..4507)
                     /locus_tag="PLW08_06355"
     CDS             complement(2114..4507)
                     /locus_tag="PLW08_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52257.1"
                     /translation="MKKYLLLILSIILATQIFAVALMNISPQVYSFHQKCDLKVEVQQ
                     GLSDIAKMKVYYRIGKSNRWMTEEMKQDSPGSPYFIATLPADYLTTDVIEYYFCVELS
                     QGNKEYFPPQNETVPNYILEPDIAFGEAEPGFVLLTDDPIISADDGYMLVVSFFAISE
                     EIDPASIEVWVGGKNVTSLSQISPPLIVYKDETPQSGTQKAVIRARKEGKTVHSPIWS
                     TEILPSSKALKQFTYQGTVNFASNYYNYSNDDALGYTKSDAATWADFYGNYGILNMQA
                     NLYFSSLEHSNSQPVNRYTLGFNIPHLEVFAGDYAPTFSQLTLNGKNLRGIYSRLYYK
                     SLSLSVTHGQTVRKTTNELDLDSEVDGMQKSGTFKQEAFGTRLQIGNEESFCIGFNLT
                     RHNDIISSLDSTYYKYEKVDEKGDKSTIYTTKAQENAVLGVDTQMHIRKPNLVIGAEI
                     ASSLWNTNTIPGAMTQEELEEYLGKDFFLDPQDLSRLFIINKNMIPIVPGRVSIAWLT
                     YVRTCFYNNLLNFQYSETGSAFNAFGSSYQIPDSKVISFTDNFNVSKYFILSGGLNLI
                     EDNLMKHKSETNNTTSWYLQSLIRIPQAPYFKFAYYNNSGKNEENKDIAYEKESFQKV
                     KNKSQNLIFGIGYNITQIPYVPTQVDISYQMGTDNNKLSDSDLTDNKNNNFNITMLNR
                     FQMIPLTLQFAMTLNNNKDLLAITNKKGKNNNFFVGATYSLWEDKIKPYTNYRIVSAS
                     GAQGDRSYQYYTLGVEAYPIKSLSVNTNIALTDFNNKDNTPGDYSNFIWRVLLSQRF"
     gene            complement(4520..5197)
                     /locus_tag="PLW08_06360"
     CDS             complement(4520..5197)
                     /locus_tag="PLW08_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPL52258.1"
                     /translation="MKKIFLFAIILILTVNLLAVESVAIISASKGKVDLRRAAKVIKH
                     KTGDFLQNNDEIRTGGESFAAYKYIDGSATVKVFSHSIVQVYASTKDKKLAKTVKLNQ
                     GSVFSQIKSDSGPFSVQTPTTVASVKGTGFMAKFDEQEQTKFIVTEGELELKILDKTE
                     TQSVAAGNTAIIQADGSFEIRPSTEEDIQEIELTELESMQEKEIRKMRIPVVAPNGQT
                     RYIDITW"
     gene            complement(5210..>5914)
                     /locus_tag="PLW08_06365"
     CDS             complement(5210..>5914)
                     /locus_tag="PLW08_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52259.1"
                     /translation="AVLAGYFPDGVLKFKVWVREFSTAPWTNDPNLPYDEFKIIIRNA
                     GNITLLSPGVPIGKTPALVNGIPISFLWNSQLTDFNDYKLIIKEFPPNNPPTVNTIDR
                     TGAVFYETPENTKENSGFADFLPFTDGNYYAWKVTTALATEYNPKKQNTGNNILSSNW
                     YVFQFVDEEKAAASISEFQAHLNMLQNSILLNLYTQGYVPAGIVIIDGKEFSGQEAIN
                     LIDALQGQEISVELKD"
     assembly_gap    5915..6014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6015..6444)
                     /locus_tag="PLW08_06370"
     CDS             complement(<6015..6444)
                     /locus_tag="PLW08_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52260.1"
                     /translation="MKRFIFILVIALLLPTLAVAREGFEVAFSHKFIKVNSFNSASFD
                     PFKPKLQPILTYLRITNTGTVPKNIDLKLVLEWNNILLVETIFSSKNPQNSWKLTNRD
                     LITNDASTYFDYKQGYSKITVKDAIENSSTLKSAVLAGYFP"
     gene            complement(6448..7392)
                     /locus_tag="PLW08_06375"
     CDS             complement(6448..7392)
                     /locus_tag="PLW08_06375"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPL52261.1"
                     /translation="MEKIVVLKGGTSPEREVSLVSGAEIASALRNMGYSVIEIDPADY
                     PKLSDLLTAIQNEQPLLVFNGLHGGSGENGELQAALSLAGIKFTGSGFKASCFSMDKY
                     ISKLIALAEGIPVPKYILMREDLLEDYQDVEDYSGFSNQLGLPIIVKPNDAGSSVGIS
                     RVECLEDLKPAVQKALQYSHSVLLEEYIPGRELTVTIIDSEAYPVVEIKPLEGWYDYT
                     NKYTHGKTKYEAPAQIDDTVAKLLQLYALRLWKAFGLSGYARIDFRYDGFKPYFLEVN
                     TLPGMTSLSLTPMAAKAAGMSFQELLQKIIYITAKEGR"
     gene            complement(7457..8368)
                     /locus_tag="PLW08_06380"
     CDS             complement(7457..8368)
                     /locus_tag="PLW08_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52262.1"
                     /translation="MLEIELTYPKPVPNRLLNIWSNPVFMQTVAEISNFIPWHLVCYK
                     GTTIVAVLPLYEKKILGCRVLKRSSLSYYQGLNLWLEENSLPARKLLDALQITMAIAR
                     GLKSNFKRIRINLTPETYDIRGFLWNKMQAVPFYTFVHNLNSELKPLPDKRRDLAQAE
                     KQNYTFEEGLHIEEFIGLLKILKVKKNWNPDCDYQGLGDFIRILYREGMLRQMNLTQQ
                     GKIVSSNLILQDGKKVYNIYQATEDNALKKGACSLHTVKLIELLQKEGFQELDFCGSS
                     IPEIARFNSALGLQLKLFFQIRSSAMV"
     gene            complement(8368..8724)
                     /locus_tag="PLW08_06385"
     CDS             complement(8368..8724)
                     /locus_tag="PLW08_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL52263.1"
                     /translation="MKPKLLLVDDDQLLREVIGDFLSSHGYEVDLADNADSALEKFEP
                     GKYQLALIDYMMPGMNGLELMKILLQQDPKIFCLIMTGYPTVDSAFQSVVDGASDYIV
                     KPFQLKELLNTIGSYI"
     gene            8897..9109
                     /locus_tag="PLW08_06390"
     CDS             8897..9109
                     /locus_tag="PLW08_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPL52264.1"
                     /translation="MKGKVKWFNKNKGYGFIITDDNKEYFVHWKSIVTNSPRELKVLE
                     QDELVTFDLMETDKGIQAINIIRVNP"
     gene            9174..10817
                     /locus_tag="PLW08_06395"
     CDS             9174..10817
                     /locus_tag="PLW08_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPL52265.1"
                     /translation="MPFLPVNQSDLKERDWDYLDIILISGDAYIDHPSFAAAIIGRTL
                     EAAGFRVGIIPQPDWRNDSSFTVLGKPRLFFGITAGNMDSMVNHYTAQRKLRNDDAYS
                     PDGISGKRPDRAIIIYTNIIKRLFKGVPIVIGGIESSLRRIAHYDFWQDKVRNSILAD
                     SKADILIYGMGERPVTELAILLQKGIPVSSIQNLPSTVVFSSTFPAKEDIILPEAEKC
                     SAKETFYQMTRQFEDNYRTKTIFQKTAGRWLKHNPPAKPLTTKEMDSIYALSFKYQPH
                     PVYAGHKIPAYEQIKDSITSHRGCYGGCNFCAIACHQGRNIQSRSETSLLKEAQKHTG
                     TITDVGGPTANMYASFCRLDFPETCKRSSCLVPDICPNLQINHSVQLKILAKIGNLPR
                     IKHLFIASGIRYDMAVNDDNYIEALATKYTGGRLKLAPEHSVSSVLRLMNKPQIEVFE
                     KFCKKFYFYTNKAGLKSKIIPYLIIGHPGTTMEDAFELRNWLRKNKLKVEQVQEFTPT
                     PMTISTCMYYTGLDYETGKPIHIPKPSEIRRQKELIINR"
BASE COUNT         3512 a         2076 c         2064 g         3584 t
ORIGIN      
        1 ttgttaatac ctgtcgttca tacggaactt tgatgaataa atggattcct actgccgcag
       61 ggagggcaag gagctgtcaa tttgaaataa tattagattg ttacttgagc tgatgaactc
      121 atatatttta acaatataat atattaacca taagggggaa aatatggttt attacttcaa
      181 gctgacgagc tcatatattt taacggcgat attttttcct ttaaccttca cttcatccag
      241 gtaattggca tttaccaggt ctttaacttt ttcataagtg aactcactga tgatgatatg
      301 attgaaagtg ttatattctt tatttagtcc ttccaatcgt gctcccaaat tgattgtatc
      361 gccaatagca gtataatcaa agatttgttc gctacccaga tttcccacaa cagccattcc
      421 ggtattaata ccgataccta tttcaaaggg ttcaatgcct tcgctttgcc atttttcctg
      481 taagcgggaa agacattctc tcatttgcaa agctgtttta caggcatcaa aagaatgatt
      541 aggcaaagga acgggtgttc cataaagagc cataatttca tcccctacaa atttatccag
      601 aataccatga ttgttgatta ttattttaac catttcggta aggtattcgt ggaggatatt
      661 gactgtttcc tgaggagaat gagtttcgga ataagtagta aaattgcgga tatcggaaaa
      721 caggacagtt atttcctgta aagaaccgcc gaattttaag cttttaggat tggcaagaag
      781 ttgattaacg agttccggag caagatattg ctgaaaagca tttctgataa agcgtttttc
      841 cttcatcgtt acccaatagt gatttaagat gctggcaaca taaataagaa tcaaaaggat
      901 aatcgtttgc gctatgggac aaataattcc agctttgtta aacagcaaca gattgataat
      961 aaataaaata atcagcagtc caagaagagc taaagcggaa aactggggtt ttaacacttt
     1021 aaaaagaaac caaagcccaa tcagcaaaac aacttcaatc agtaaaaaca gccagggatt
     1081 aagtttcttc aagtaatttc ccgttaacac catatccaga aaattggctt gaatttctac
     1141 tcccgaagtc cattcgcctc cataaggagt gggaaattta tcgtgcaatt catcaattgt
     1201 agctccaacc aacacaattt tatcttttaa aacgccacta tctcttattt cgtaaaattc
     1261 atccaattcg gcaccctgat aaccaggcat aaaaaaagtt gaatcatcca aaatactggc
     1321 ataagaaaaa gcaggataag ttttagcagg acctctgaaa ttgatgattg ccttgttttt
     1381 atctgcaagg ggaatacttt tatcggttaa atgcaatgcc ttgctgttaa ctttaatatg
     1441 ctcagcccaa ttgttttgat agacccttaa atttgccaga gatgcaattc ctatactata
     1501 gagtttggtt tgatcaaagt tttcaaaaag ggtgtattta cgaataaaac catccggatc
     1561 agcttccata tttacaattc cccaaggcaa attatattct gttatgggac taatgggagg
     1621 aataatctga ctgggttctc cgggacgctg tgaagttaag acctttccgg caaaaattac
     1681 attctgatat ctatttgcag tttctgccag ccgtaaatca ctttccggca aagttttttc
     1741 cgtaaactca atatcaaata taatctgctt ggctccggct ttatttagat tttctattaa
     1801 tttggaatga tagtcccggg gaaagggcca ggtttcattt aaagcgctaa aggtggcatc
     1861 atcaatacct ataataacaa ttttatcgga aaccggtaat ttacctctca ttcggaaaag
     1921 catatcctga gctttgtatt ccagattctt aaagaaagga aaagcaaaga taatttctac
     1981 gattagcagg attataacga ggtaaataat tacttttttt acgggcatct aaacctcctg
     2041 aataaaaagc ccgtcttgca gaaaaataac tttctgcaaa acggaagcaa taaattgtaa
     2101 gtcattattc aaattagaac cgctgtgaaa gcagaactct ccaaataaaa ttactgtaat
     2161 caccaggggt gttatcttta ttattaaaat cggttaaagc gatattagta tttacgctta
     2221 aggactttat agggtatgct tcaactccta aagtgtaata ttgataactt ctatcacctt
     2281 gagctccact ggcagaaaca atccgataat tggtataggg ctttatttta tcttcccaca
     2341 agctgtatgt tgctccaaca aagaaattat tattttttcc ctttttattg gttattgcca
     2401 aaaggtcttt attgttattc agtgtcatag caaactgcag ggtaagagga atcatttgga
     2461 aacgatttaa catcgtgata ttgaaattgt tgtttttatt atccgttaag tccgaatcgc
     2521 ttagtttgtt gttatcggtt cccatttgat aactaatatc cacttgcgta ggcacataag
     2581 gaatttgagt aatattataa ccgatgccga agattaagtt ttgggacttg tttttaactt
     2641 tttggaacga ctctttttcg tatgcgatat ccttgttttc ctcatttttg ccggaattat
     2701 tgtaataggc aaatttgaaa tatggagctt gaggtatgcg aatgagagat tggagatacc
     2761 aggaagttgt attattggtt tcacttttat gcttcatcag gttatcttca atcaaattca
     2821 aacctccgct taagataaaa tatttagaaa cattgaagtt atccgtaaag gagatgactt
     2881 tgctatcggg aatttgatag gaagaaccaa aagcattgaa agcagaacca gtttcggaat
     2941 attggaaatt caagagatta ttataaaagc aagttctaac ataagtaagc caggcaatac
     3001 tgacacgacc tggcacaata ggaatcatat ttttattgat gataaacaat ctgcttaaat
     3061 cctgaggatc gaggaagaaa tctttgccga gatattcttc cagttcttcc tgtgtcattg
     3121 ctcccggaat agtgtttgta ttccaaagcg agctagcaat ttcagctcct ataaccaaat
     3181 ttggtttacg gatatgcatc tgggtatcta ctcccaaaac tgcattctct tgtgctttag
     3241 tagtgtaaat agtgcttttg tcaccttttt catccacttt ttcatatttg tagtaagtac
     3301 tatccagaga actgattata tcattatgac gagttaaatt aaaaccgatg caaaaggact
     3361 cttcattgcc aatttgtaaa cgggttccaa atgcctcctg tttgaaggtt ccagatttct
     3421 gcatcccgtc tacttcagaa tcaagatcaa gttcgtttgt cgttttgcga actgtctgtc
     3481 cgtgagttac agataacgat aaggatttgt aatataagcg ggaataaatt ccccttaagt
     3541 tttttccatt caaagttaat tgactgaagg tcggagcata atcaccagca aaaacttcca
     3601 gatgaggaat attgaaaccc agggtataac gattcacagg ttgactgtta ctatgctcta
     3661 atgatgagaa atagaggttt gcctgcatat ttaatattcc gtagtttcca tagaaatcag
     3721 cccaggtagc ggcatcgctt tttgtgtaac cgagagcatc atcattggag taattataat
     3781 aattggaggc aaaattcaca gttccctgat aagtaaattg ctttagtgct ttacttgagg
     3841 gtaaaatttc ggttgaccag ataggagaat ggactgtttt tccctctttt cttgctctaa
     3901 taaccgcttt ttgggttccg ctttgaggtg tttcatcttt ataaacaatc aggggagggc
     3961 taatttgaga caaagaagta acatttttgc ctccaaccca aacctcaatg ctggcaggat
     4021 caatttcttc actaatggca aagaaagaaa cgaccagcat atagccatca tctgcagaaa
     4081 taatgggatc atcggttaaa agaacaaaac cgggttctgc ttctccgaag gcaatatccg
     4141 gttccagaat gtagttgggc actgtttcat tttgtggagg aaagtattct ttatttccct
     4201 ggctcaattc aacgcaaaag taatattcta tcacatcagt agttaaataa tcagcaggca
     4261 gagtagcaat aaaatagggc gaaccaggac tatcctgttt catttcttcg gtcatccagc
     4321 gattggattt accgatacga taatagacct tcattttggc tatatcgctt aaaccctgtt
     4381 gcacttccac cttcaaatcg catttttgat ggaaagaata aacctgaggc gaaatattca
     4441 tcaaggcaac ggcaaaaatt tgagtagcga ggataattga taatatcagc aataaatatt
     4501 ttttcatctt tatatcctcc taccaggtaa tatcaatata cctggtttgg ccattaggag
     4561 caacaaccgg tatacgcatt ttcctgattt ctttttcctg cattgattcc agttctgtca
     4621 attcaatttc ctggatgtct tcttccgtac tgggtctgat ttcaaaactg ccatctgcct
     4681 gaatgatagc agtatttcct gcagcaactg attgggtttc ggttttatcc aaaattttca
     4741 attccaattc cccttcggta acaatgaatt tggtctgttc ctgctcatca aatttagcca
     4801 taaaaccggt tcctttaacg gaagcaacag tagttggcgt ttgcacggaa aagggtccgg
     4861 aatcactttt tatttgggaa aaaacgctcc cctggtttag tttaactgtt ttggcaagct
     4921 ttttgtcttt ggtagaagca taaacctgca cgatagaatg ggaaaagacc tttacagtag
     4981 cagaaccgtc aatatattta taagcagcaa aggattctcc tccggtgcgt atttcatcat
     5041 tattctgcaa aaaatcaccg gttttatgtt taatcacttt tgctgctctg cgtaaatcca
     5101 ctttaccttt agaggcggaa ataatagcta ctgattccac agctaataaa ttaacggtta
     5161 aaatgaggat gatagcgaaa agaaatatct ttttcatttt tcctcctctt cagtccttca
     5221 attccactga aatttcctgt ccctgcaaag catcaatcag atttatagct tcctgtcctg
     5281 aaaattcttt accatctata atcacaattc ctgcaggaac ataaccttgc gtgtaaagat
     5341 ttaacaagat actattttgt agcatattga ggtgtgcctg aaattctgaa atactggcgg
     5401 cggctttttc ttcatccaca aactggaaaa cataccagtt actggaaagg atattattac
     5461 cggtgttttg cttttttggg ttatattctg ttgccagagc agttgttact ttccaggcat
     5521 aatagttgcc atctgtaaaa ggcaggaaat cagcaaaacc gctgttttct tttgtattct
     5581 ctggggtttc ataaaaaacg gctcctgttc tgtctatagt atttacggta ggcggattgt
     5641 taggaggaaa ctctttgata atgagcttat aatcgttaaa atcagtcagc tgtgaattcc
     5701 ataaaaagct tatgggaatt ccgttaacta aagctggtgt tttaccaata ggaactccag
     5761 gagaaagcaa agtaatattt ccggcattac gaattatgat tttgaattca tcataaggta
     5821 aattaggatc attagtccaa ggagcagtgg aaaattctct tacccaaacc ttgaatttaa
     5881 ggactccatc gggaaaataa cctgccaaaa cagcnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnncgggaa aataacctgc caaaacagcc gattttaagg tggaactgtt
     6061 ttctattgca tcttttactg ttatttttga atatccttgt ttataatcaa aataggtgct
     6121 tgcatcgttg gttattaaat ccctgttagt taatttccag gaattctgag gattcttgga
     6181 gctgaaaata gtttctacca acagaatatt attccactcc agaactaatt tcaggtctat
     6241 atttttagga accgtgcctg tatttgtaat tcttagatag gttagaatag gttgtagttt
     6301 cggcttaaaa ggatcaaaac ttgcagagtt gaaactgttt accttaatga acttatgtga
     6361 gaaagcaacc tcaaaacctt ctcttgccac agctaatgtt ggcaataaca atgcgataac
     6421 taaaataaaa ataaagcgtt tcatttttta cctcccttcc ttagcagtaa tatagattat
     6481 tttttgcagt aattcctgaa aagacattcc cgctgctttt gcagccatag gagttaaaga
     6541 aagcgaagtc attccaggca gtgtattgac ttccaaaaaa tagggtttga agccatcgta
     6601 gcgaaaatca attctggcat aaccacttaa accgaatgct ttccataacc ttaaagcata
     6661 gagctgtaaa agtttggcta cggtatcatc aatttgagcg ggtgcttcgt atttagtttt
     6721 tccgtgagta tatttattgg tataatcata ccaaccttcc aggggtttga tttccactac
     6781 aggatatgcc tccgaatcaa taattgtgac ggttaattcc ctaccgggga tatattcttc
     6841 taaaagaacg ctatgtgaat attgcaatgc cttttggaca gcaggtttca agtcctccaa
     6901 acattcaact ctgcttattc ctacggaaga accggcatca tttggtttta caattatagg
     6961 aagtcccaac tgattggaaa atccggaata atcttccaca tcctgataat cctccagaag
     7021 gtcttctcgc attaaaatat atttgggaac cggaattcct tccgccaaag caatcagctt
     7081 ggaaatgtat ttatccatac tgaaacagct ggctttgaaa ccggaacctg taaacttaat
     7141 tcctgccagg gaaagagctg cctgcagttc accattttca ccgctacctc catgcaaacc
     7201 attaaagacc agtaatggct gttcattttg aatggcagta agtaaatcag aaagtttcgg
     7261 gtaatcggca gggtctattt ctattactga atagcccata tttcttaagg cagaagcaat
     7321 ttcggcaccg ctaaccaaag agacctctct ttcaggagaa gttccccctt tcaagacaac
     7381 aattttttcc attattactt atcctttatt gcctgcagag ttcttattcc gcaaacaggt
     7441 aagttttcct tttttattat accatagcag aagacctaat ttgaaagaaa agcttcagct
     7501 gaagaccaag tgcactattg aatctggcaa tttcaggtat gctgctacca cagaaatcca
     7561 gttcctgaaa accttctttt tggagaagtt ctatcagttt aaccgtatgc aaactacagg
     7621 cacctttttt taaggcatta tcttcagttg cctgatagat attatatacc tttttgccat
     7681 cctgtaagat aagattactg ctaactattt taccttgttg ggttaaattc atttgccgta
     7741 acattccttc ccgatataaa atacggatga aatcacccaa tccctggtaa tcgcaatcgg
     7801 gattccagtt ttttttaacc ttcagaattt ttagtaagcc gataaattcc tcaatatgca
     7861 atccctcctc aaaagtataa ttttgttttt ccgcttgggc aaggtctctg cgtttatcgg
     7921 gaagaggttt tagttcgctg tttagattat ggacaaaagt ataaaaagga acagcttgca
     7981 tcttattcca taaaaaacct ctaatatcgt aggtttcagg agttaagttg attctaattc
     8041 tcttaaagtt actttttaag ccccttgcta ttgccatcgt tatttgtagg gcatcaagca
     8101 actttcttgc cggcagagaa ttttcctcca gccaaaggtt taatccctga taataggata
     8161 aagaggagcg ttttaacact ctgcaaccaa gtattttttt ctcgtaaagc ggtaaaacag
     8221 caacaattgt agtgccttta taacagacca gatgccaggg tatgaagttg gatatttcag
     8281 ctactgtctg cataaaaaca ggattgctcc aaatatttaa taagcggttg ggaacgggtt
     8341 tgggatatgt taattctatt tccagcatca gatgtaggaa ccgattgtat ttaataattc
     8401 ttttagttga aagggcttta caatataatc ggaagcaccg tctacaacgg attgaaaagc
     8461 cgaatcaaca gtagggtaac cagtcataat cagacaaaag attttcgggt cttgttgcaa
     8521 taaaattttc attaactcca atccattcat tccaggcatc atataatcaa ttaatgccag
     8581 ttggtattta ccaggttcaa acttttccaa agcgctatct gcattgtcag ccaaatcaac
     8641 ttcataaccg tgggatgata aaaaatcacc tattacttct cgtagtaatt gatcatcgtc
     8701 cactaataga agttttggct tcatataaac tcctctgaat ttgttgatta cctcatataa
     8761 aactatgata atcagttctc ttaaatagtc aatagaaaaa tgttaatagg gtcgtgaggt
     8821 cttgcctttt ttttaattga cataatcagc cctatttatt ttaatgacat taggataaaa
     8881 atatgtgagg ttcaatatga aaggaaaagt aaaatggttt aacaagaata aaggttatgg
     8941 ctttattatt actgatgaca acaaggaata ctttgtccac tggaaatcta tagttaccaa
     9001 ttctccgcga gaactgaaag ttttggagca ggatgaactg gttacttttg acttgatgga
     9061 aacggacaaa ggcattcagg caatcaatat tatcagagtt aatccctgat tagatgccta
     9121 ccttttttca ggggtaatgg aaagttataa aatccattac ccctttttta gcgatgcctt
     9181 ttcttccagt taaccaatca gacctgaaag agagggattg ggattattta gacatcattc
     9241 ttatcagtgg agatgcctat atagaccacc cttcttttgc tgcggcaatt atcggacgca
     9301 ctttagaagc agccggtttt agagttggaa ttattcctca accggattgg agaaatgatt
     9361 cctcttttac cgttttagga aaaccgcgtc tcttttttgg cataactgca ggcaatatgg
     9421 attctatggt aaatcattac acagcccaga gaaaactgcg taatgacgat gcttacagtc
     9481 ctgatggaat ttccggaaag cgtcctgaca gagctatcat tatctatacc aatatcataa
     9541 agcgtctttt taagggtgtg ccaattgtta ttggaggtat agaatcatcc ttgcgtcgga
     9601 ttgctcatta tgatttttgg caggataaag ttcgcaatag cattttggca gatagcaaag
     9661 cggatatttt aatttacgga atgggggaga gacctgtaac ggaactggcg attttattgc
     9721 aaaaaggaat tcctgtaagc agtattcaaa atttgccttc cactgttgtt ttttcttcta
     9781 cttttccagc aaaggaggat attattttgc cagaagcaga gaagtgttcc gctaaagaga
     9841 ccttttatca aatgaccaga caatttgagg ataattatag aacaaaaact atctttcaaa
     9901 agacagcagg acgctggctg aagcataatc caccggcaaa accattaact acaaaggaaa
     9961 tggatagcat ttatgccttg tcttttaaat atcaaccgca tcctgtttat gcagggcaca
    10021 aaattcctgc ttatgaacaa attaaggatt ctattacttc tcatcgcgga tgttatggtg
    10081 gctgcaattt ttgtgctatt gcttgtcatc agggacgcaa tattcaatcc agatcggaga
    10141 cctctctgct gaaagaagca caaaaacata ccggcacaat taccgatgtg ggtggtccta
    10201 ctgcaaatat gtatgcttca ttttgccgtt tggattttcc tgagacctgt aaaagaagtt
    10261 cctgtctggt tccagatatt tgtcccaatt tgcaaataaa tcattcggtt cagttgaaaa
    10321 tcctcgctaa aatagggaat ttaccccgca tcaaacattt atttattgcc tccggaattc
    10381 gctatgatat ggcagtaaat gatgataact atattgaagc ccttgctact aaatatacgg
    10441 gagggcggtt aaaacttgcg ccggagcatt ctgtttccag tgttttacgc ttgatgaata
    10501 aaccgcagat tgaggtcttt gaaaagtttt gtaaaaagtt ttatttttac actaataaag
    10561 cgggactaaa aagcaaaatt attccttacc tgataattgg tcatccggga actactatgg
    10621 aagatgcttt tgaacttcgt aattggctaa gaaaaaataa attaaaggta gaacaagtgc
    10681 aggaatttac ccctacacca atgacaattt ccacctgtat gtattatacc ggtttagatt
    10741 atgaaacagg aaagccgatt catattccta aaccctctga aatacgcagg caaaaggaac
    10801 taattataaa ccggtagaat gacatccttg tcgttcattt tgtagttgtt aggcagtaag
    10861 gaataaatat attgtaaatt agaggtaaac aaaaagtggt gttcataatt ctatttattc
    10921 ttataggata cggcttcata aagttatata agttatctaa gccatataat ccttttcacc
    10981 caacaggaac aataatagat gaatatctcc aaaaaggtaa tcgtaacatc aatatagaga
    11041 taagaataag agatagggac gaaaaagatt agaagtttaa cattgttata gtttccaaga
    11101 gcaatacaat gatagcgtgg agagcataaa cctttcctta aaacccaata atgcaataga
    11161 aataattgtt aatatcattt ctccattcgt taagcacata ttgtagtaaa aatttcgtag
    11221 aaagcaaatt agattagttc cctattgaaa attcagttat atagtttaaa aatattggga
    11281 ttcttgattt cgcgggaaag acattagaat gatagatatt atggaaagat ttgaac
//
LOCUS       DAOOZV010000059        11024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_5791_length_11024_cov_64.327104, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000059 DAOOZV010000000
VERSION     DAOOZV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_11024_cov_64.327104"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(507..1148)
                     /locus_tag="PLW08_06400"
     CDS             complement(507..1148)
                     /locus_tag="PLW08_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL52266.1"
                     /translation="MKKYVFITLGLLALAFCFAEESPVTLYLQNQNLETYQQAVNYLI
                     KIDQEGESGMQAKLNLAYISNLESLRLMDLAKADIENLKPGERFALANLYLEMDKYDD
                     AIDIYNLLNEATPKWSCPWRHKGEALYKSGNYEAAKKSLEMAIETNKEHYDAYLWYAK
                     TLYELKDYKNALKALETAFDLTEELEDSGFDQVIPEEEINQLYQELQKLTQNK"
     gene            complement(1163..1444)
                     /locus_tag="PLW08_06405"
     CDS             complement(1163..1444)
                     /locus_tag="PLW08_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPL52267.1"
                     /translation="MLTVNEYFEGKVKSIAFQNKQGNFTVGVMDIGEFEFGTNTIERM
                     TVISGELTVQLPEEETWKKFTSKETFTVPAHKKFRVKVQEQTAYLCRYE"
     gene            complement(1515..1589)
                     /locus_tag="PLW08_06410"
     tRNA            complement(1515..1589)
                     /locus_tag="PLW08_06410"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1555..1557),aa:Asn,seq:gtt)
     gene            complement(1746..2117)
                     /locus_tag="PLW08_06415"
     CDS             complement(1746..2117)
                     /locus_tag="PLW08_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52268.1"
                     /translation="MTAKRFYIAVLMGIILGIIACLIAGGVNPNPLAKKVLANIFVSR
                     VLIGFVIGISAWKMGWFLHGVLMGLIVGLPFSLSAMAIQVPGMGKWGIFFLTWALGVV
                     YGFIIELVTSVFFKAEQPSAK"
     gene            complement(2299..3618)
                     /locus_tag="PLW08_06420"
     CDS             complement(2299..3618)
                     /locus_tag="PLW08_06420"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPL52269.1"
                     /translation="MNNFNPVELLIKKRDGYSLAPEEISCFVNAYLKGEIADYQMSAF
                     LMACFLQGLDKQEIEALTKTYIDSGGTISFAEGLPVADKHSTGGVGDKISLMLAPIAA
                     SLGLMVPMISGRGLGHTGGTLDKLESILGFRTSFSLQEFKELVEKHSFALAGQSEELV
                     PADKKIYALRDVTATVESPGLITASIMSKKIAEGAKHLVIDLKIGSGAFMPNINKAKE
                     LAELLMATGKSFGQKVTVVFSNMNSPLGHAVGNALEMIEAIEYLKGNYLPDTYELTTV
                     LTAQMLISTGKAKDYEQAKKMIDEAVVSGKALEKFREIIIAQGGEPKVLEDYSLFASS
                     PVIYPIIAKQSGWVHKIDCRAIGYALVHIKAGRKKVTDTLDYGAGALLYPKIGDAIKQ
                     GEKIGEVHCASKNEAEEVAELILNAYKIADTQKDKEDLILEIALSEE"
     gene            complement(3630..4601)
                     /gene="phnE"
                     /locus_tag="PLW08_06425"
     CDS             complement(3630..4601)
                     /gene="phnE"
                     /locus_tag="PLW08_06425"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HPL52270.1"
                     /translation="MVKKLKILGIEYALWLYIVACICWFLFRVFSISASSYIIFISPL
                     IIAVLLSQCKNNIGAKIIISEETGSLWKIELAIVILITFIMAWVIVDVKPIAFFTQAA
                     NTKSIISGIFNPNLRQLVPMLSALVETIYLALLATVFAIPFAFVLSFFAAKNLMFSSF
                     IGRIVYIIIRTISTVFRSIEAIVWAIIFCVWVGIGPFAGMLALWIHSIAALIKLYSEQ
                     IENIDPGPVEAIKATGASTLQVWRYGVTPQILAPYLAFTIYRWDMNIRMATVVGFVGG
                     GGIGLALYQEQQMLAWRNVGLIMWLIALIVWVMDMLSGYLRSKLVTY"
     gene            4769..5359
                     /locus_tag="PLW08_06430"
     CDS             4769..5359
                     /locus_tag="PLW08_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52271.1"
                     /translation="MTNLSEIKMCPQVINNMRSLSCVRKKTGFLNCPVMPLMLFIFIF
                     SLAFGTAFAQNKSEEYLEVKTWKEPKDAVPFKELVLQDSIYLYKDKPFTGVAIERYPN
                     GFLQRAATFVKGKQNGPMLMWYPDGSPQMSANYVNGYLHGRFLGWYNNGSVIYDLMIN
                     RGTYTGDNLFDEDASRASSEIEDREGESPDNDQSQE"
     gene            5340..>6154
                     /gene="panB"
                     /locus_tag="PLW08_06435"
     CDS             5340..>6154
                     /gene="panB"
                     /locus_tag="PLW08_06435"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPL52272.1"
                     /translation="MTKVKNNKTDIAVFKEMKQHQEKICMLTAYDYAMAKCVSATSVD
                     IILVGDSLGMVVLGYEDTLQVTLENMIYHCSAVRRGAPDSFIIADMPYLSFHLSEKQT
                     RQNAGKLIVQGKANAVKLEGGSKSRIDAIKAIVDMEIPVCAHLGLTPQSIRKFGGYKV
                     QGKKPVEQEELLKQAQEVAKAGAFMLVLEGIPELLGKEITETVSIPTIGIGAGRFTDG
                     QVLVYHDLLGYSDLQPKYVKRYADLNNNIIEAVELFSQEVKNGVFPAPENIYYP"
     assembly_gap    6155..6254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6255..6812
                     /locus_tag="PLW08_06440"
     CDS             <6255..6812
                     /locus_tag="PLW08_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52273.1"
                     /translation="NLKDKLEDNFISLGQLLSEIKRSKLYLYKGYENFKDFVEMEYQL
                     SGTLAGKLMSTFDLFIEEMDIDETEVKEIGFDRLQMIKPFMKNADWNVRDEWIHKAEE
                     MPFKELREHIKELKQKEKESNIDLKEVYIEQFWEKMTGWFNCSRKELNFKLALYFQDA
                     DLDNIKKIIKERQHLFELETQNKKE"
     gene            6819..8378
                     /locus_tag="PLW08_06445"
     CDS             6819..8378
                     /locus_tag="PLW08_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPL52274.1"
                     /translation="MKQMQILWVDDEIDLLKPFILFLEERKYNVETCNNGTDAIEKVV
                     ENKYDLVILDEMMPGLDGLATLQEIKRINPALPIVMVTKSEAEGLMDKAIASQISDYL
                     IKPINPNQIIMAIKKIFQADEIRANQIGQQYTRYIAELNQKLFANPDWNEWSQIYREL
                     SYWELQIDQVNDEGLRQTHFLEKRNCNTEFTNYVERNYSNWLKSDERPNLSFDLISQY
                     IAPHFEENLPIYFIIIDCMRLDQYLAIQPYIKELFEEELDLYYSILPTATPYSRNSIF
                     SGLMPIDIAKRFPDYWVTSSEMDNSRNRNEHQLLDEHIEELGFKLDPTSKYVKIFNME
                     EGNFVLRKIETWNKENLIVLVYNFLDLLAHHRSRDQILQETIPNEEGLRAFTKHWFLH
                     SSLYEALKLIAKQDAIVILSTDHGSIKVNRATQVIGDKETSITVRYKEGKNLSVNERH
                     ALFVKKPGDFGLPSKSIVDNFIFAKDDYYFVYPNAYHQYQKQFNGTFQHGGVSMEEMI
                     LPIATCRTKRR"
     gene            8849..9283
                     /gene="tsaE"
                     /locus_tag="PLW08_06450"
     CDS             8849..9283
                     /gene="tsaE"
                     /locus_tag="PLW08_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPL52275.1"
                     /translation="MKTLNLSTEQDTIDLAKYIAPLLKEGDIITLFGDLGSGKTFFVK
                     QLGKALGIEEEIDSPSFVLMKEYSGGRLTLYHLDLYRLKNKEEIYCLGLFDILEQGIT
                     VIEWPLLVNDLLPYQTLKLEFHFDGKKRWVDIIPDKEHSPYF"
     gene            9301..10083
                     /locus_tag="PLW08_06455"
     CDS             9301..10083
                     /locus_tag="PLW08_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPL52276.1"
                     /translation="MITALLVIICIAIAAGIIWLIFTKDKVVARTWGIVGLSFIIGLT
                     VFGYFFMQSRQAPTSLRVVGYASKLFESDLVKWNLTMQRNVSHDGLKDGYNKMSNDVN
                     AFKNYLEEQGIPEKDISIQPINSYQTTDNYGNPTGYALNQNIFVVSSDIPKIETLALK
                     PGFFAERGILLLNSNLAYLYTKLPDLKKQLLSEATKDAVARAEEIAGSGNTKLGKLRE
                     ARAGVFQITEPYSTEVSDYGIYNTSTRSKSISVTLTAIFKLK"
     gene            10186..10938
                     /locus_tag="PLW08_06460"
     CDS             10186..10938
                     /locus_tag="PLW08_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL52277.1"
                     /translation="MKLRRIFIALGVALIALFSLSCSPKQKKILRVGTDAENPPFTYL
                     ENGEFKGIDIEICKRIADKLNMQLQITKYQFDELFSALALNKIDIAASAITITPKHKQ
                     TMDFTIPYFETDLAVVAKSNSPVKVEKIEDLGKYIVGCQSNTTSHQYLTENLMEKDLL
                     PKNKLKLYPTVIETLGELLNGKIDLMVFDEWVAKDFSQQQEIKIVYTIQTNEQYGLAM
                     QAGKEINTRINKALKELKDSGELENIIKEYID"
BASE COUNT         3488 a         2124 c         2030 g         3282 t
ORIGIN      
        1 acgccaccat cgctatcatt gtgtcgccct ttggggcttt tggaaaatgg gaagaagaaa
       61 agaaaaaaga aatcagggct tacattttct gaggggctaa cgccaccatc gctatcattg
      121 tgtcgccctt tggggctttt ggaaaatggg aagaagaaaa gaaaaaagaa gtcggggctt
      181 acatttaacg aggggcttac gcacccatcg ctatcattgt atcgccctcc gggctttttg
      241 ggaaaaggaa aagaaaaaag aaattagtga ttgtgtcttt attttttcga ggggcttacg
      301 cacccatcgc tatcattgtg tcgccctccg ggcttttttt gcagagggca gagagctgag
      361 ggcagagagc atctcaacct tatcaaccct ctcaaacttc tcaaccttct aaaccttctc
      421 aaccttatca accctctcaa acttccaaac acttctgtat tctcgcctgt tcaggaagga
      481 caatgtattt tttgccagaa atggtattac ttattttggg ttaatttctg cagttcctga
      541 taaagttgat tgatttcttc ttccggaatt acctgatcaa atccgctatc ttcaagttct
      601 tcagttaagt caaaagcggt ttccagagct ttaagagcgt ttttatagtc ctttaattca
      661 tagagggttt tggcatacca gaggtaagca tcataatgtt ctttattggt ttcaattgcc
      721 atttccagtg actttttagc ggcttcataa tttccgcttt tataaagagc ttctccttta
      781 tgacgccagg ggcagctcca ttttggtgtt gcttcgttca aaagattata aatatcaata
      841 gcatcgtcat atttatccat ttccagataa agatttgcca gggcaaagcg ttcgccgggt
      901 ttcaaattct caatatccgc ttttgccaaa tccattaacc ggagagattc caaattgctg
      961 atataggcaa gattcagttt tgcctgcatt ccgctttcgc cttcctgatc aattttgatg
     1021 agataattaa ctgcttgctg ataggtctcc agattttgat tttgcaggta taaagtgacc
     1081 ggtgattctt ctgcaaaaca gaaagctaat gccaacaatc ccaatgtgat aaatacatat
     1141 tttttcattt tatcaatcct cattattcat aacggcaaag atatgctgtt tgttcttgta
     1201 cttttacccg gaatttttta tgggcaggaa cggtgaatgt ttctttggaa gtaaattttt
     1261 tccaagtttc ttcttcgggt aattggacgg taagttctcc ggaaataacg gtcattcttt
     1321 caattgtatt agtgccaaat tcaaattccc caatatccat tactcctacg gtgaagttgc
     1381 cttgtttgtt ttgaaaagca atggacttta ctttaccctc aaaatattcg tttacagtta
     1441 gcatttttgc ctccttaata ctcttttttt gattgcacca cttatccaac acttaaattt
     1501 ggtttataaa aaagtggctc cggaagaggg gttcgaaccc ccgacctagt ggttaacagc
     1561 cacccgctct accgctgagc tattccggaa cacatttcaa gaatacttaa tttttaaggt
     1621 cgtgattttg tcaatccaaa ttttttttgt agccggagca ggcaacgctc cgccttttta
     1681 gtgatttttt tgcggaggaa tagcgtcctc cgactacact tggggaaatg aattaaattc
     1741 gctatctatt ttgccgaggg ttgttctgcc ttaaagaaaa cgctggttac aagctcaatg
     1801 ataaatccgt atacaactcc caaagcccag gtcaggaaaa atattcccca tttccccata
     1861 cctggaactt gtatagccat agcacttaag gaaaaaggca agccaacaat cagtcccatt
     1921 aaaactccat gtaaaaacca gcccattttc caagcactaa ttccaatcac aaaaccaatt
     1981 aaaacacggc ttacaaaaat attggcaagc actttcttag ctaaggggtt ggggtttact
     2041 cctcctgcta ttaagcaggc aataattccc aatatgatcc ccattaaaac ggcgatgtaa
     2101 aaacgtttgg cagtcatatt aacctcctaa tactgtgcta tttatattat atagcaagag
     2161 caggggaaaa gcaaattatt tttacagctt ttatataaaa tgaatagtta tcgtcttgaa
     2221 aaagcagtca agttgtaata ttttacctaa attctgcttg tgcaggaatg acaaacgact
     2281 tttctttctt aatatgattt attcttcaga taaagcgatt tccaggatga ggtcttcttt
     2341 atctttttgg gtatccgcta ttttataggc attcaaaatt agttctgcta cttcttccgc
     2401 ctcattttta gaggcacaat gcacttctcc tattttttct ccttgtttta tagcatctcc
     2461 aattttagga taaagtaaag caccggctcc ataatcaagg gtgtccgtaa ctttttttct
     2521 gcctgctttg atatgaacta aggcatagcc aatagctctg caatcaattt tatgcaccca
     2581 accactttgt tttgctatga taggataaat tacaggtgaa gaggcaaaaa gggaataatc
     2641 ctccagcact tttggttctc ccccttgagc aataattatt tcgcggaatt tttccagtgc
     2701 ttttccgctg acaactgctt catcaatcat cttttttgcc tgttcatagt cctttgcctt
     2761 gccagtgctg atgagcattt gagcagttaa aacagtagta agttcataag tatcgggtaa
     2821 atagtttcct ttcaggtatt caattgcctc aatcatttcc aaagcatttc caacagcgtg
     2881 tcccaaagga gaattcatat tggaaaagac cacggttact ttttgcccga aactttttcc
     2941 tgttgccatc aataattcag ccagttcttt tgctttattg atattaggca taaaagcacc
     3001 gctgcctatt tttaagtcaa taaccagatg ctttgctcct tcggcaattt tcttgctcat
     3061 tatgctggca gtaatcaacc ccggactttc tactgtagcg gtaacatctc ttaaggcata
     3121 tattttttta tcagcaggaa caagttcctc agattgacct gctaaagcaa aactgtgttt
     3181 ttctaccagt tccttaaatt cctgcagaga aaaggaagtt ctaaatccta aaatggattc
     3241 cagtttatct aaagtaccac ctgtatgacc caaaccgcgt cctgaaatca ttggcaccat
     3301 caatcctaaa gaagcggcaa taggagccag cattaaactg attttatctc ctacgcctcc
     3361 ggtagaatgt ttatcggcaa caggcagacc ttctgcaaaa ctaatagtgc ctccggaatc
     3421 aatataggtc ttggttaatg cctctatttc ttgtttatcc agaccttgca gaaaacaagc
     3481 cattaaaaaa gcgctcattt ggtaatcggc aatttctcct tttaaatagg catttacgaa
     3541 gcaagaaatt tcctccggag caagagaata accatcacgc tttttgatta acagttcaac
     3601 cggattaaaa ttattcattc tgcaatctcc taataggtaa caagtttgga acgaaggtaa
     3661 ccacttaaca tatccattac ccaaacaatt aaagcaatta accacattat taaacctaca
     3721 tttctccagg caagcatttg ctgttcctga tataaagcca aaccaattcc tccgccacct
     3781 acaaaaccta caactgtagc cattctgata ttcatatccc aacggtaaat tgtaaaagcc
     3841 aaatagggtg ccaaaatttg aggagtaact ccataacgcc atacctgtaa agttgaagca
     3901 cccgttgctt ttatagcttc cacaggaccc ggatcaatat tttcaatctg ctcactgtat
     3961 aattttatca aagcagctat ggaatgtatc cataaagcta acattccggc aaaaggacca
     4021 ataccaaccc aaacacaaaa aataattgcc cacacaattg cttcaatact tctgaagaca
     4081 gtagaaatcg ttcggataat aatatacact atcctgccga taaaagaact gaacatcaga
     4141 ttcttggcag caaaaaagga aagcacaaag gcaaaaggaa tagcaaaaac ggttgccagt
     4201 aaagccagat aaatagtttc tactaaagca ctaagcatcg gaacaagctg tctcaaattc
     4261 gggttaaata ttccactgat aatggacttt gtattggctg cctgagtaaa aaaagcgatt
     4321 ggctttacat ctacaattac ccaagccata atgaaagtaa taaggattac tattgccagc
     4381 tcaatcttcc acagtgaacc tgtctcttcc gagatgatta tttttgctcc tatattattt
     4441 ttgcattgag acaaaagaac tgctatgatt aacggactga taaaaataat atagctactg
     4501 gcggaaatac tgaaaacccg aaataaaaac cagcagatac aggcaactat gtataaccag
     4561 agggcatatt caatacccaa tatttttagt ttcttcacca taaaactatt tttacacagt
     4621 gatttattag gtcaaggaaa aaactctgga agaacaacat tcctgtcgtt ttgtgcttga
     4681 tgaggacatc aacctttcag ttgactttaa gataatttgg tttatcttat ataataaaaa
     4741 gcttcagtta aaaaagagaa gaaaaagatt gactaaccta tcggagataa aaatgtgtcc
     4801 tcaagtgata aataatatgc gttccctatc ctgtgtccga aaaaaaaccg gcttcctaaa
     4861 ctgcccggta atgcctttaa tgctattcat ttttatattt tcccttgctt ttggcactgc
     4921 ctttgcacag aataaaagtg aagaatattt agaggttaaa acttggaagg aaccgaaaga
     4981 tgcggttcct tttaaggaac ttgttttgca agacagtatt tatctttata aagataaacc
     5041 cttcacagga gttgccatag aacgatatcc caatggtttt ttacaacggg cagcaacttt
     5101 tgtaaaaggt aaacagaacg gtcctatgct gatgtggtat cctgatggca gtccccaaat
     5161 gagtgctaat tatgtaaatg gttatttaca cgggcgtttt ctgggctggt ataataatgg
     5221 cagtgttatt tatgatttaa tgattaacag gggaacttat accggtgata atctttttga
     5281 tgaagatgca agccgtgcaa gttctgaaat agaagatagg gaaggagaaa gtcccgataa
     5341 tgaccaaagt caagaataat aaaacagata ttgctgtttt taaggaaatg aaacagcacc
     5401 aagaaaaaat ctgtatgctt actgcctacg attatgcgat ggcaaaatgt gtttctgcca
     5461 cttccgtaga tatcatcctg gtaggtgatt ctttggggat ggtagttttg ggctatgaag
     5521 ataccctgca agtaactttg gaaaatatga tttatcattg ttctgcagtt cgtaggggtg
     5581 caccggattc ttttattata gccgatatgc cctatcttag ctttcatctt tccgaaaagc
     5641 aaaccaggca aaatgcagga aagcttattg tgcaaggaaa agctaatgcc gttaaactgg
     5701 aaggtggctc aaaatctcgt attgatgcca taaaagccat tgtggatatg gaaatacctg
     5761 tttgtgccca tttaggttta acaccacagt ccattcgtaa atttggcgga tataaagttc
     5821 agggaaagaa accggttgaa caggaagaac tattaaagca agcacaggaa gttgccaaag
     5881 caggtgcctt tatgcttgtt ctggaaggca ttcctgaact tttaggcaaa gaaataactg
     5941 aaacggtaag cattcccaca atcggaatcg gagccggaag atttaccgat ggacaggttt
     6001 tagtttatca cgacctgctt ggttattcag atctccagcc caaatatgta aaacgctatg
     6061 ccgatttgaa taataatatt atagaagcgg tagaactctt ttcccaggaa gttaaaaacg
     6121 gagttttccc cgctcctgaa aatatttatt acccnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnaatctg aaagataaac tggaagataa ctttatctct ctcggtcagt
     6301 tgctttccga aatcaagcgt tcaaaattat acctttataa gggctatgag aattttaagg
     6361 actttgtgga aatggaatat caactttccg gaaccctggc aggaaaactt atgtctacct
     6421 ttgatctgtt tattgaagaa atggatatag acgaaaccga ggttaaagaa atcggttttg
     6481 atcgtctgca gatgattaaa ccctttatga aaaatgcgga ctggaatgtg cgtgatgaat
     6541 ggattcataa agcggaggaa atgcctttta aggaactccg cgaacacata aaagaactga
     6601 aacaaaaaga aaaagaatca aacatagacc tgaaagaggt ctatattgaa caattttggg
     6661 agaagatgac aggatggttt aattgttccc gcaaagagct taatttcaag ctcgcccttt
     6721 attttcaaga tgccgatctg gacaacatta aaaagatcat taaagagcgc caacacctct
     6781 ttgaacttga aacacaaaac aaaaaggagt aaaatccaat gaaacaaatg caaatactct
     6841 gggttgacga tgaaatagac cttctcaaac cctttattct cttcctggaa gagagaaaat
     6901 ataatgttga aacctgtaat aatggaaccg acgctattga aaaggtagta gagaataaat
     6961 atgacctcgt tattttagac gaaatgatgc ccggcttgga tggcttggca actttacagg
     7021 aaattaagcg tatcaatcct gccttgccta ttgtaatggt tactaaaagt gaagcagagg
     7081 gcttgatgga taaggcaatt gcttctcaaa tttcggatta tttgattaag cccatcaatc
     7141 ccaatcagat tattatggct atcaaaaaga ttttccaagc agatgaaatt cgtgccaatc
     7201 aaataggcca acaatatacc cgctatattg cagaactgaa tcaaaaactt tttgccaatc
     7261 ccgattggaa tgaatggagc caaatttacc gagaactgag ctattgggaa ttacaaatag
     7321 atcaggttaa cgatgaaggt ttacgccaaa cccattttct ggaaaaacgc aactgtaata
     7381 ctgaatttac caactatgta gaacgcaatt atagtaactg gctgaaaagt gatgaacgcc
     7441 ccaatttaag ttttgacctt atttcccaat acatagctcc gcactttgaa gaaaacctgc
     7501 ctatctattt tattataatt gattgtatgc gtctggatca gtatctggct attcaaccct
     7561 acatcaagga actttttgag gaagaacttg acctctacta ttccattttg ccaactgcta
     7621 ctccctatag cagaaattcc attttcagcg gtttaatgcc tattgatatt gctaaacgct
     7681 ttcccgatta ttgggtaact tccagtgaaa tggataatag ccgtaatcgc aatgaacatc
     7741 aattgctgga tgaacatatt gaggagcttg gctttaaact tgatcctacc agcaagtatg
     7801 taaaaatctt caatatggaa gaaggcaatt ttgtgctgcg caaaattgaa acttggaaca
     7861 aggaaaatct cattgttctt gtttataact tccttgatct attagctcat catcgcagtc
     7921 gggatcaaat tctacaggaa accattccca atgaagaagg tctgcgtgct tttaccaaac
     7981 actggttttt gcattcttcg ctctatgaag ctttgaaact gattgccaaa caagatgcga
     8041 ttgtaattct ttctacagac cacggctcta taaaagtgaa ccgtgcaact caagtaatcg
     8101 gtgataaaga aacatccata acagtccgtt ataaagaagg aaaaaatctt tccgtgaatg
     8161 aacgccacgc tctgtttgta aaaaaacccg gtgactttgg actgccctcc aaaagcattg
     8221 tggataactt tatctttgcc aaggatgatt actatttcgt gtatcccaat gcttatcatc
     8281 aatatcagaa acagtttaac ggcacttttc aacacggtgg ggtctctatg gaagaaatga
     8341 ttctgccgat tgctacctgc cgcaccaaaa gaagatagtt gtttatgaag agaaagaatg
     8401 tagaatgaag gaagtcagga gtcgtcatcg cttatatatc tttaaccctt atctcaaatt
     8461 agtttagcga tgaggactct tgacgggaaa ggaagacaaa atgtaaaatg aaatcagaag
     8521 tgcagaaaaa ttgtatagcc ggagctccgc aaattccaaa tctttctttg tcacagagga
     8581 acactgtcat cagtgattta ggtgtaaaaa tatatgactc ctgacgaaga gaaaagagat
     8641 taaaggtaga atacacagaa aatgtttatt aaccgtatgc tttccctatc aaaatgctgt
     8701 tacccaaagg taagaccttg gcaaactttt tcttttctct tttccgatag gtttgatagg
     8761 ggaattgcag atattttgat tcttgctttc gcaggaatga caaattttcc atctcactat
     8821 attcatttat cattcttttt aagctgagat gaaaacccta aacttatcta ctgagcagga
     8881 cactatagac cttgccaaat acattgcacc gttattaaag gaaggggata ttataactct
     8941 ttttggcgat ttgggtagcg gcaaaacctt ctttgtaaaa cagcttggca aggcactggg
     9001 aattgaagaa gaaatagaca gtccttcttt tgttttaatg aaggaatatt ccggtggacg
     9061 ccttaccctg tatcatttag acctataccg tctgaaaaat aaagaagaaa tatattgcct
     9121 gggtttgttt gatatcttgg aacaaggaat aaccgttatt gaatggcctc ttttagtaaa
     9181 tgatctttta ccctatcaga cattgaaact ggaattccac tttgatggaa aaaaacgctg
     9241 ggtggatatt attcccgata aagagcattc tccatatttt taagttttaa ggagcaaaaa
     9301 atgatcacag cactattagt aatcatctgt atcgcaattg cggcaggtat aatctggctt
     9361 attttcacca aggataaagt agttgcccgc acctggggaa ttgttggttt atcctttatc
     9421 atcggtttga cggtttttgg ctatttcttt atgcaaagtc gtcaggcacc aaccagttta
     9481 cgtgtagttg gatatgccag caaactcttt gaatccgatt tggtaaaatg gaatctcaca
     9541 atgcaacgca atgtaagcca tgatggtttg aaagatggct acaacaaaat gagcaatgat
     9601 gtaaatgctt tcaagaatta ccttgaagaa cagggaattc cggaaaagga tatcagtatc
     9661 cagcccataa acagttatca aaccaccgat aattatggca atccaaccgg ttatgccttg
     9721 aatcaaaata tctttgtggt ctcttccgat atacccaaaa ttgagacctt ggcattaaaa
     9781 ccaggttttt ttgcggaacg cggaatctta ttgttaaatt ccaacctggc atatctttat
     9841 actaaactac cggatttgaa aaaacagttg ctttccgaag caaccaaaga tgcagttgct
     9901 cgtgcagaag aaattgccgg tagcggaaat accaaactgg gaaaattaag agaggcgcgg
     9961 gctggtgttt ttcagattac tgagccctat tctacagagg tttcggatta tggaatttat
    10021 aacaccagca ctcgcagtaa aagcatttcc gttactttaa ctgctatttt taagttaaag
    10081 tagatttttt tccacaaacc tgcttgtaat tttacggata aaatacttgt aatttatccg
    10141 agggggatta taatttatat aaaaccatat agtaaaggta agaagatgaa attacgcagg
    10201 atatttattg ctttgggtgt cgcactgatt gccttatttt ctttatcttg ttctccgaaa
    10261 caaaagaaga tattgagggt aggaaccgat gccgaaaatc caccttttac ttatctggaa
    10321 aacggtgaat ttaaaggtat tgatatagaa atatgtaaac gcatagcgga taaattgaat
    10381 atgcaattac aaattaccaa ataccaattt gatgaactgt tttctgcttt agcacttaac
    10441 aaaattgata ttgctgcctc tgctattacc attaccccga aacacaaaca aacgatggat
    10501 tttactattc cttattttga aacagacctg gctgttgtag ccaaatccaa ttctcctgta
    10561 aaagtagaaa agattgagga cctcggaaaa tacatcgtgg gctgccaaag caacactacc
    10621 agccatcaat atttaaccga aaacctgatg gaaaaagacc tcttgcccaa aaataagctg
    10681 aaactttatc ctactgttat agaaaccttg ggggaacttc ttaatggcaa aatagacctg
    10741 atggtttttg atgaatgggt agccaaggat ttttcccaac aacaggagat taagattgtt
    10801 tacacgattc aaacgaatga acaatacgga ttggctatgc aagcaggtaa agaaattaat
    10861 accagaataa ataaggcatt gaaggaatta aaagatagcg gagaattgga aaacattatc
    10921 aaagaatata ttgattaaat acctcgcaca gattacccgg atttcacacg gataataaat
    10981 ataagcacgc agatttcgca gattacgcag atttttcatt ttat
//
LOCUS       DAOOZV010000060        10677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6027_length_10677_cov_56.784410, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000060 DAOOZV010000000
VERSION     DAOOZV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6027_length_10677_cov_56.784410"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..2906
                     /gene="polA"
                     /locus_tag="PLW08_06465"
     CDS             150..2906
                     /gene="polA"
                     /locus_tag="PLW08_06465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPL52278.1"
                     /translation="MADKLYLIDGTALLYRAHFAFIKNPLINSKGMHTSAIFGVINSF
                     LHILENTQAAYVAISFDRKAPTFRHNLYEAYKAQRPPMPDELTAQIEPVKRFFELIGL
                     KEIGLDGYEADDILGTLAKQYKDQFQIVFLTSDKDYCQLVDERSVMLDPMKDIVLDRD
                     AVYAKYGVYPEQFIDYLALVGDPSDNIPGVSSIGPKTAAMLLSEYKTLDNIYANLDKL
                     KPRLQEVLRENKENAYLSRQLAKIDTDVPIPKPDLEHLTFWAKDLRKAIPLLEEYEIN
                     SIKRKIELKYPETNATKQQTEYQDDIFKEKEKPQEETTETEEKMPFSAVLVNTLNFVT
                     LLKELKEAKQISLDTETDSPEPMRANLVGISFCMDEKKAYYLPLGHQLAVNLPLPEVL
                     RHLEDALKGKLLLGHNLKFDLIVLARNGLDLDNPLFDTMLAAYILDPGTLNYSLETCA
                     LNELNYKMIPISDLIGKGKNQSTFDLVEPEKACIYSAEDAWAVYKLYPVYRRKVDFSV
                     MANLFDTIELPLIKVLQQMEMNGVAIDCSVLSEISHQINLELKTLTDKIYAFAGYEFN
                     INSTQQLAKLLFEEKKLPYGRKTKTGYSTDNSVLEELAIDYEIAGIIIQYRQLSKLQS
                     TYVSALPKMINPQTGRIHSSFNQTVTSTGRLSSSNPNLQNIPVRTEIGREIRKAFCAK
                     DAEHLILDADYSQIELRLLALMSEDEVLIDAFKQDLDIHKRMAAKIFNVDIEKVTPDQ
                     RRAAKTINFGILYGMGQRKLSRELGISLNEAKDIIDGYYAQFPSIRNFINQCVNKARQ
                     QHYAETLFGRRLYLPNIDSKNQGLKSEAERIAVNMPIQGTAADLIKIAMLNIHKQIDK
                     REDIKMILQVHDELLFEVQQEALKETKNIVKTCMESALPPKYAEKVHLKTDVGVGKNW
                     FEAH"
     gene            complement(3006..3728)
                     /locus_tag="PLW08_06470"
     CDS             complement(3006..3728)
                     /locus_tag="PLW08_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52279.1"
                     /translation="MKRLVILLLALLLLAGCVQYDEELWINRNGSGHATIRVVHRSPY
                     ENPEEIMRKAALPGINLQSYDIQRKGPDVIYTINFKFKSIEAFNNVNDQLGAADFWGK
                     ITLNKEPGRRITFKRRIALGSQEQEEEGIEDILGLLQPENPVWTYKVHLPWKIISTNA
                     LPENVDRENGTVTWSFDTRKMWHKQELMTVELQKDFPWFLLVIGAVIVVLLIFLIHWM
                     FRFPKKSHWRERLKHSENVEKS"
     gene            3892..4269
                     /locus_tag="PLW08_06475"
     CDS             3892..4269
                     /locus_tag="PLW08_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPL52280.1"
                     /translation="MIALRQMWHCPICGNVVEVIYVGGGELVCCGQPMKLLKENTVDA
                     AVEKHIPVVTDLGDKIEVSVGSIPHPMEEKHYITCIEVLTEKKVLRKELKPGDEPKAK
                     FCVKMDKVIAVREYCNVHGLWKA"
     gene            4289..4879
                     /locus_tag="PLW08_06480"
     CDS             4289..4879
                     /locus_tag="PLW08_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPL52281.1"
                     /translation="MPNKETRTAENLMKSFAGESQARMRYLYSAKTAKNEGFEQISNI
                     FTETAENEKEHAKIFFKHLQKLGLEGEIINIISSYPVGWSADSTLKNLEYAANGENEE
                     WTELYPIFADVAEEEGFSDIAHSWRMVAKVEREHEKRFRKLYDNIKNQRVFIREEKVF
                     WKCLNCGYIHEGTEAPKSCPSCNHPQSYFELFMENY"
     gene            4900..5040
                     /locus_tag="PLW08_06485"
     CDS             4900..5040
                     /locus_tag="PLW08_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPL52282.1"
                     /translation="MQKYQCMACGWIYDPAENDNIPFEDLPDDFVCPECGVGKDMFEP
                     LD"
     gene            5121..5573
                     /locus_tag="PLW08_06490"
     CDS             5121..5573
                     /locus_tag="PLW08_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL52283.1"
                     /translation="MQRKDFEEILDFAIAREREAIKFYQNLQNQAQFQDQKEMLKELE
                     AMEQNHITIIEKLHQTGVKDEDIHKTPNLKISEYLTADPETLDLSYQNILIKGMKREE
                     TSFLLYSEMSVKFPDPEISTLFRRLAADEAQHKKYFENLYDDWMRKGN"
     gene            5570..6244
                     /gene="tsaB"
                     /locus_tag="PLW08_06495"
     CDS             5570..6244
                     /gene="tsaB"
                     /locus_tag="PLW08_06495"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPL52284.1"
                     /translation="MKLALDTTQNFGSIALADEKRIIYSAYFDIKITHSETLMPAIDY
                     AFQFCNVERRELKEIYVCIGPGSFTGLRIGLATAKGIAFALGLPLYAFSSLELSALPA
                     SRLGKNILSAIDAKMKEVYFAYYDQAIKEIIPPAIMKPEELCQLHLDDFILCGSATEM
                     LSPLLQKAGYNFHNLNPIMKIPSAAGLFFLPEMLPEKYVPQDIEDLEPMYLREAQAQV
                     KKKKEN"
     gene            6388..10554
                     /locus_tag="PLW08_06500"
     CDS             6388..10554
                     /locus_tag="PLW08_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPL52285.1"
                     /translation="MRKTILCLLMLIVSISIIYGDYFVNFEGTNETKTAYASGNVTLS
                     GLSWNMTEALIGNLANDWKNGTRSARLRGYGTSAMTMLENKTNGLGTLSFYYRRYSTE
                     AQVDWKVEYSIDNGSSWTQIGSPFTAPASDTPSLFSEQVNVSGNVRIRIKRATESGTS
                     DRRLNIDDITLTDYTAAEPTLILDPTVLIGFTYDLGEGPSASQSYTLSGAYLSPSSGN
                     ITITGSSSFEVSSDNVTFSNSFSLPYSGGILSPATLYVRLKAGLSEGNYLNENITHSG
                     GGASVFLAVSGSVTSTSLLLTAEGYLEDFTSFTSLESLPFGWTLSNQYTYGGDFGTGS
                     AGGLRGNGVLGIQLTASAPNNALTATLTLKNQTGATINNLNVAYQGKVARTDQPGTPK
                     WVVSVNGNEIPELEYSTAEGINQQKSAVVTGLNIAVGENIIIQWFTTSAGTSGARRQI
                     GIDDVNINTNIPVNPLINVTANLVTFQTAQGTPSEPQSYFLSGIDLSQNINISAPNGF
                     EISVDGGLTYATYTSVLPTYEGNVFVRMTGTAVGTFGGNIIHTSLGATDVYLPVAGIV
                     TGATGYAVDLFFSEYIEGSSSNKSIEIFNGTGSEVNLSDYKVALYSNGSTTPSSSLIL
                     SGTLENGDVYVISHPSANANILAVTDLTNNIINFNGNDALALIKISTGAYVDIFGVIG
                     NDPGTAWLGENNYTTINSTLVRKSSVAQGITQNPTGTGAYAFTTLVTEWDLYPVDTID
                     NLGFHNFAATPQEIEPPTVQVSNLIAYPGNTDITLEWTPGNGARRLIKINTINSFTPP
                     VDGSNPTANNVYSGNGEQVIFNGATQIVEGIPLNGCNVSGLTPATTYWFRSYEYNGSG
                     SNTRYNTSEAINNPISATTTNTQSSGYYAGITGYGNTLKTNLHNLLRTTHTTRYSYDA
                     LWIKLPYTDEDPNNTDNIIEIYTGWSVPKSYYGGGTSQWNREHTWSKSHGDFGETPPA
                     GTDLHHLRPCDSTVNSAKSNKDFDYAPNPYIDSSPYNGYSSDTGCKTSTYAWEPRDED
                     KGDVARMIMYMAIRYEGTDTSFDLEIVDNINTSGPNYGKLSTLLQWHIIDPPDAREMQ
                     RNNRIQELQGNRNPFIDEPMYAYQIWAPMPLNPTNITQTGFTANWTAPISATKYYFQL
                     ATDANFTNIVPGYDNLDVGLTLSRNISGLSNGYTYYYRLRSYFTSGYSMYSPYREVTL
                     TLPASAQLSSGTALTEGNLNGAILQLTLTNTTWRDNTLLISSFTLNNAPAGLSLLSVQ
                     YLSATAAQIVLSFDNTDFDANILNFSVTINALEINNGSNLTTNTLTIIAYIECPLSIQ
                     IQGSQLILNIQEVSGANLYKVFSSTDPYGVYTEISTSGIFDPLVPNRWSYTVPSDERR
                     FYRSAAVRNP"
BASE COUNT         3410 a         2038 c         2083 g         3146 t
ORIGIN      
        1 taaactctct aaaccatctc aaccttatca atcctctcaa cttttttcat tctaattctt
       61 tatcgcgctt tgcagaattc ccttgccaaa aaatcacttt ccctattgtg ttatcaagta
      121 agaaaatcaa gccaaaggac ggttaggaaa tggctgataa attatattta attgatggca
      181 cggcattgtt atacagggct cattttgcct tcattaagaa tcctttaatc aacagcaagg
      241 gaatgcatac cagtgctatt ttcggagtaa taaattcgtt tctgcacatc ctggaaaata
      301 cacaagcggc ttatgtagcc atatcttttg accgtaaagc tcctactttt cggcataatc
      361 tttatgaggc atacaaagct cagcgtcctc ctatgccaga tgaacttacg gctcaaattg
      421 aacccgtgaa acgatttttt gagttaatcg gcttaaagga aatcggtctt gatggttatg
      481 aagcggatga cattctgggg actttggcaa agcaatataa agatcagttt caaattgtgt
      541 ttttaaccag tgacaaggat tactgtcaat tggttgatga gcgttcagtt atgcttgatc
      601 cgatgaaaga tattgttttg gatcgggatg ccgtatatgc taaatatgga gtttatccgg
      661 agcagtttat tgattatctt gcgcttgttg gagacccttc tgacaacatt cctggcgtga
      721 gcagcatcgg acctaaaact gctgcaatgc tactttccga atataaaacg ctggataata
      781 tctatgccaa tttggataag ttaaaaccac gcttgcagga agtccttcgg gaaaataagg
      841 aaaatgctta tctctcaagg caattagcaa aaattgacac cgatgttccg attcccaaac
      901 ccgatttaga gcacctgact ttttgggcaa aggatctgag aaaagcaatt cctttgctgg
      961 aggaatatga aattaattcc attaagcgga aaatagagct gaaatatccg gaaaccaatg
     1021 caacaaaaca acaaaccgaa tatcaggacg atatttttaa ggaaaaggaa aagccacaag
     1081 aggaaacgac agaaacagag gaaaaaatgc ctttttcagc tgttttggtt aacactctca
     1141 attttgttac tctgctcaaa gaactgaaag aggcaaaaca aatttctctt gatacggaaa
     1201 cggattcccc ggaacctatg cgggcaaatt tggtgggaat atccttttgt atggatgaaa
     1261 agaaagcata ctatcttcca ttgggacatc aacttgcagt aaatttgccg ctaccggaag
     1321 ttcttcgcca tttggaagat gccttaaaag ggaaactttt actgggacat aatctcaagt
     1381 ttgatttaat agttcttgcc cgcaatggat tagaccttga taatccactg tttgatacaa
     1441 tgctggctgc ctacattctt gatcctggaa cgctgaatta ctctctggaa acttgcgctc
     1501 tgaatgaatt gaattataaa atgataccta ttagtgactt aattggtaaa ggcaaaaacc
     1561 agagcacttt tgacctggta gaaccggaaa aagcatgtat ctactctgca gaagatgcct
     1621 gggctgttta taaactctat cctgtttacc gaagaaaagt agattttagc gttatggcaa
     1681 acctttttga tacgattgaa ctccccctta tcaaggtctt acaacagatg gaaatgaacg
     1741 gagtggcaat tgactgttct gttttaagcg aaatatccca tcagataaat ctggaactga
     1801 agaccctgac agataaaata tatgcgtttg cgggatatga atttaacatc aattctaccc
     1861 agcaattggc aaaactgctc tttgaagaaa aaaaactacc ctacggtaga aaaaccaaaa
     1921 ccggctattc tacggataac agtgtgctgg aagaacttgc cattgactat gaaattgccg
     1981 gaataattat tcaatacaga cagctttcaa aactgcaatc tacttatgta agcgctctgc
     2041 ctaaaatgat caatccccaa accggaagaa tacattcctc ttttaatcaa accgttactt
     2101 ccacaggtcg cctttcttct tccaatccca atctgcaaaa tatacccgtg cgcacagaaa
     2161 ttggacgcga aatccggaaa gctttttgtg ccaaagatgc agaacaccta atccttgatg
     2221 cagattattc acaaatagaa ttgcgtttgc tggctctgat gagcgaagat gaggtcttaa
     2281 tagatgcttt taaacaagac ctggatattc ataaaagaat ggctgccaaa atttttaatg
     2341 tggacattga aaaggttact cccgaccaaa gaagagctgc aaaaaccatt aactttggca
     2401 ttctctacgg aatgggacaa cgcaaacttt cccgcgaact gggaatctct ctcaatgaag
     2461 caaaagacat tatagacggt tactatgccc aattcccttc cattcgtaat tttatcaacc
     2521 aatgtgtaaa taaagctcgt caacaacatt atgcggaaac cctttttggc agacgattat
     2581 accttcctaa tatagacagt aaaaatcagg gactaaaaag cgaagcggaa agaattgcgg
     2641 ttaatatgcc aattcaagga actgcagctg acctgattaa aatagctatg ctcaatattc
     2701 ataagcagat agataaacgc gaagacatca aaatgattct ccaagtacat gatgaactcc
     2761 tttttgaggt tcagcaagaa gcattgaaag agacaaaaaa tatcgttaag acctgtatgg
     2821 aaagtgctct acccccaaaa tatgcggaaa aagtccatct gaaaacggat gtgggagtag
     2881 gaaaaaattg gtttgaagca cactgaagaa tgaaaaatgt taagataaaa aagctaataa
     2941 atcctcttca tcccatacat attaaatctg tgaaatctgt gttatctgtg agagataatc
     3001 tttttttagc tcttttctac attttcgcta tgctttagtc gttccctcca atgtgatttt
     3061 ttcggaaagc ggaacatcca atgaatcagg aaaataagca agacaacaat tacagcccca
     3121 ataactaaga gaaaccaggg aaaatccttt tgcaattcaa cagtcattaa ttcctgttta
     3181 tgccacattt tacgggtatc aaaactccag gtaacagtcc cgttttccct atccacattt
     3241 tccggtagag cattggtaga aataattttc cagggaagat gaactttata tgtccaaaca
     3301 ggattttcgg gttgcagcaa acccaatata tcttcaatac cttcttcttc ctgttcctgg
     3361 cttccaagtg caattctgcg tttgaaagta attcttctgc ctggctcttt atttaaggtt
     3421 attttccccc aaaaatcagc agcacctaat tgatcgttta cattattgaa ggcctcaatg
     3481 cttttaaact tgaaattgat ggtgtaaata acatcaggac cttttctctg aatgtcgtag
     3541 gattgtaaat tgatacctgg taatgctgct ttacgcataa tttcttccgg attttcataa
     3601 ggtgaacgat gaactacacg aatagttgca tgtcctgacc cgttacggtt aatccatagt
     3661 tcttcatcgt attgaacgca tccagctaaa agcagaagcg ctaacagcag tattacaagg
     3721 cgtttcattc cccaaccttt ttcctaattt aatactttgt aaagcataaa aaccaaaagc
     3781 gtttttttgg caagaaaatt cttgattatt aaacggtaaa aaattcttgc ttgacaaaac
     3841 tatcaatcta ttataacaag gaaaatgcat attaacttaa ggaggaataa gatgatagct
     3901 ttaagacaaa tgtggcattg cccgatttgc ggaaatgttg ttgaagtaat ttatgttggc
     3961 ggtggcgaat tagtttgctg tggtcaacca atgaaattac ttaaagaaaa tactgttgat
     4021 gccgcagtag aaaaacatat acccgttgta acggatttgg gagataaaat tgaggtctcc
     4081 gttggaagta tccctcatcc gatggaagaa aagcactata ttacctgtat tgaagtgctc
     4141 acggaaaaaa aggttttacg caaagaactg aagccaggag atgaacctaa agcaaagttt
     4201 tgcgtgaaaa tggacaaagt tattgctgtg cgtgaatatt gcaatgttca cggtttatgg
     4261 aaagcataaa caattgaagg aggaaaatat gcccaacaaa gaaaccagaa ctgccgaaaa
     4321 tctgatgaaa tcctttgcgg gagaaagtca agccagaatg agataccttt attcagctaa
     4381 aacagctaag aatgagggct ttgaacaaat ttccaatatc tttacagaga ccgcggaaaa
     4441 tgaaaaagaa catgccaaga tatttttcaa acatctgcaa aaactgggtc tggaaggcga
     4501 aattatcaat attatctcat cctatcccgt tggctggtct gcagatagca cacttaaaaa
     4561 tttggaatat gcagctaatg gagaaaatga agaatggaca gagctttatc ccatctttgc
     4621 cgatgtggct gaagaagagg gttttagcga tattgcccat tcctggagaa tggtagctaa
     4681 agtggaaaga gagcatgaaa agcgttttcg taagctctat gataatataa aaaatcaaag
     4741 ggtgtttatt cgtgaagaaa aggtcttctg gaaatgcttg aattgcggtt acatccatga
     4801 aggaacggaa gcacctaaga gctgtccttc ctgcaatcat cctcaaagtt attttgaact
     4861 ttttatggaa aattactaaa ctctcaagga gataataaaa tgcagaaata tcaatgtatg
     4921 gcttgtggat ggatttacga tcctgccgaa aacgacaaca ttccttttga agacctgccc
     4981 gatgattttg tttgcccgga atgtggcgtc ggaaaagata tgtttgaacc cttggattga
     5041 ataaatccgt tcccgtttaa ccggggagaa tcacatctcc ccggtggttt ttataaaact
     5101 gaaaatttgt gaggacagaa atgcaacgaa aggactttga ggaaattctg gattttgcca
     5161 tagcgcgtga gcgggaagca attaaattct atcagaattt gcaaaaccaa gcccaattcc
     5221 aggatcagaa agaaatgcta aaagaactgg aagcaatgga acaaaaccac ataacgataa
     5281 ttgaaaagct acaccagaca ggtgttaaag acgaagatat tcacaaaacc cccaatttga
     5341 aaataagcga ataccttaca gctgatcctg aaactttaga cctaagctat caaaatatcc
     5401 ttattaaagg tatgaaacgc gaagaaacat cctttctgct ttattccgaa atgagcgtaa
     5461 aatttcccga tcctgaaata tctacccttt tcagacgcct tgctgcagat gaagcacaac
     5521 ataaaaagta ctttgaaaat ttatatgacg attggatgcg gaaaggaaat tgaaacttgc
     5581 tcttgatact actcaaaatt tcggttccat agctttagca gacgaaaaga ggattattta
     5641 ctccgcctat tttgacatta aaataaccca tagcgagacc ttgatgccgg caattgacta
     5701 tgcttttcaa ttttgcaatg tagagcggag ggaactgaaa gaaatctatg tatgcattgg
     5761 acccggttct tttaccggac taagaattgg cttagcaaca gccaaaggaa ttgcttttgc
     5821 tttgggtctt cctctttatg cgttttcttc tttggaactt tctgctttac cggcaagtcg
     5881 tttaggtaag aacattttat ctgccataga tgctaaaatg aaagaagtgt attttgccta
     5941 ttacgatcag gcaattaaag aaattattcc tcctgcgatt atgaaaccag aagaactttg
     6001 ccaattgcat ttagacgatt tcatcctttg cggaagcgct acggaaatgc ttagtccctt
     6061 attacaaaaa gccggatata acttccataa tcttaatccc attatgaaaa taccttctgc
     6121 agcaggactt tttttcttgc cagaaatgct gccggaaaaa tatgttcccc aagacattga
     6181 agatttggaa ccaatgtatt tgagagaagc ccaagcgcag gttaaaaaga agaaggaaaa
     6241 ttgaaaaaag tgagagggaa aacaaagaga taaagagaaa aagagaagaa ttttataatt
     6301 attaatttaa taatccgtgt ttatccgtta aatctatgtt gctaatacat attttcggtg
     6361 tcttcagtgt aataaggaga atataaaatg cgaaaaacaa tactctgctt actaatgcta
     6421 atcgttagca tctctattat ctatggagat tactttgtca attttgaagg gacaaatgaa
     6481 acaaaaactg cttatgcctc aggaaatgta actttaagcg gactttcctg gaatatgaca
     6541 gaggcattaa ttggcaattt agctaacgac tggaagaatg gaactcgttc tgcccgactg
     6601 cggggttatg gaacatcagc aatgacaatg ctggaaaata aaactaacgg tttaggaact
     6661 ctttcttttt attatcgtcg ttattcaact gaagcacaag ttgactggaa agttgaatac
     6721 agcattgata atggtagttc ctggactcaa attggttctc cttttacagc tcctgccagc
     6781 gatacaccca gtttattttc cgaacaggta aatgtaagcg gtaatgttag aatcaggatc
     6841 aaacgagcta ccgaatcagg aacatcggat agacgcttaa atattgatga tataacctta
     6901 acagattata ctgctgccga accgactctt attcttgatc ctactgtttt aataggattt
     6961 acttatgacc tgggagaagg accttctgct tcacaatcat ataccttatc cggagcttat
     7021 ttaagtcctt catccggtaa tattactatc accggttcca gtagttttga ggtctcttct
     7081 gataatgtaa ctttttctaa tagtttctct ttaccttata gcggaggcat tctttccccc
     7141 gcaactttat atgtgcgttt gaaagcagga ctaagtgagg gtaattatct aaatgaaaac
     7201 atcactcata gtggaggcgg tgcttcagtt ttcttggctg ttagtgggtc tgtaacttcc
     7261 acctctcttt tactcactgc agagggttat ttggaagatt tcacctcctt tacttctctg
     7321 gagagtttgc cttttggatg gactttaagt aaccaatata cctacggagg ggattttgga
     7381 acaggttctg caggtggatt aagaggtaac ggagttttgg gaattcagct tacggcttcg
     7441 gctcccaata atgctttaac tgccactctt accttaaaaa atcaaaccgg agccactatc
     7501 aataacctta atgttgccta tcagggaaaa gttgccagaa cggatcagcc aggaactccc
     7561 aaatgggtag ttagtgtaaa cggaaatgaa attcctgaat tggaatattc tacagcggaa
     7621 ggaattaatc aacaaaaaag tgcagttgta accggtttga atatagctgt aggagagaat
     7681 attattattc aatggtttac aacgagtgca ggaaccagtg gagccagaag acaaattggc
     7741 attgacgatg taaacatcaa tactaatatt ccagttaatc ccctgatcaa tgttacagcc
     7801 aatttagtta cttttcaaac agctcaaggc acaccttccg aaccgcaaag ttattttctc
     7861 agcggaattg accttagcca aaatattaat atttcagctc ccaacggttt tgagatttct
     7921 gtagatggag gtttaactta tgctacatac acaagtgttc ttcctactta tgaaggaaat
     7981 gtttttgtcc gaatgactgg aactgctgtc ggaacttttg gcggaaacat tattcatacc
     8041 agtttgggag ctacggatgt ttatcttcct gtagcaggaa tagttaccgg agcaacaggt
     8101 tatgctgttg atttattctt ttccgaatac attgaaggca gctcttccaa taaatctatt
     8161 gagattttta acggaacagg ttctgaagta aatttatctg attataaggt agccctctat
     8221 tccaatggtt caacaactcc ttcttcttct ttaatattaa gcgggactct ggaaaacggt
     8281 gatgtatatg ttatttccca tcccagtgcc aatgcgaata ttttagccgt tacagattta
     8341 actaataata taattaattt taacggtaac gatgccttag ctctcataaa aataagcacg
     8401 ggagcttatg ttgatatttt cggtgttatc ggaaacgacc ctggaactgc ctggttaggt
     8461 gaaaacaatt acactactat aaattctacc ttagtaagaa agtcctctgt agctcaaggt
     8521 ataacgcaaa atccaaccgg cacaggtgct tacgctttta ctactttggt taccgaatgg
     8581 gatttatacc ctgtggatac aatagacaat cttggctttc ataatttcgc tgccacacct
     8641 caagaaatag aaccgccaac agtgcaagtt tcaaatttaa ttgcttatcc tggcaatacg
     8701 gatataactt tggaatggac tccgggaaat ggagcaagaa gattaattaa aatcaacact
     8761 atcaattcat ttacccctcc tgttgacggt agcaatccta ctgccaacaa tgtttacagt
     8821 ggcaacggag aacaggttat cttcaatgga gcaactcaaa ttgttgaagg aattccgtta
     8881 aatggctgta atgtatccgg tcttactcct gcaactacat attggttcag gagttatgaa
     8941 tacaacggca gtggcagtaa tactcgttat aatacttcgg aggcaataaa caatcctatt
     9001 tccgcaacta caacaaatac ccaaagcagt ggctattatg ctggaattac aggttatgga
     9061 aacacactga agacaaatct gcataattta cttcggacta cacataccac ccgttattct
     9121 tatgatgctt tgtggattaa attgccctac acggatgaag accctaataa tactgataat
     9181 attattgaaa tatataccgg ttggagtgtt cccaaatcct attatggagg aggaacttca
     9241 caatggaata gagagcatac ctggagtaaa agccatggtg attttgggga aacaccacct
     9301 gctggaacag atttacatca tttgcgtccc tgcgattcta ctgtaaattc tgccaaaagt
     9361 aataaggatt ttgattatgc tccgaaccct tatattgatt cttcacccta taatggttac
     9421 tcttcagata ctggctgtaa aacatctact tatgcctggg aaccgagaga tgaggataaa
     9481 ggtgatgtag ccagaatgat aatgtatatg gcaatccgtt atgaaggaac cgacacttct
     9541 tttgatctgg aaattgtaga taatataaat accagcggac ctaattatgg aaagctttct
     9601 actttgctgc aatggcatat tattgatcct ccggatgcgc gtgaaatgca acgtaataat
     9661 cgtattcagg aacttcaggg aaatcggaat ccctttattg atgagccaat gtatgcctat
     9721 caaatttggg ctcctatgcc tttaaatccg actaatatta ctcagacagg tttcactgct
     9781 aattggacag ctcccatttc tgctacaaag tattattttc agctggccac tgatgccaat
     9841 tttacgaata ttgttcccgg ttatgataat ctggatgtgg gtttaactct ttctcgcaat
     9901 atcagtggct tatctaatgg gtatacttat tattatcgtt tgcgttccta ttttacaagt
     9961 ggttacagta tgtattcacc ttatcgggaa gttactctta ccttacctgc ttcggcacaa
    10021 ctttcttccg gaaccgcatt aacggaagga aacctgaatg gagctatttt acaattgact
    10081 ttaaccaata ccacctggag agataacacc ttgctgatta gtagttttac tctaaataat
    10141 gctcctgcag gtctttctct tttaagcgtg caatatctca gtgctacagc cgctcaaata
    10201 gttttaagct ttgataatac cgattttgac gctaatattc ttaattttag cgttaccatc
    10261 aatgctttag aaataaataa tggctccaat ctaactacca acactttaac gattatagct
    10321 tatatagaat gccctctatc catacagatt cagggttcgc agttgatatt aaatatccaa
    10381 gaggtctccg gcgccaatct ttacaaggtc ttttccagca ctgatcccta cggtgtttat
    10441 acggaaataa gcaccagcgg aatttttgat cctcttgttc ccaatcgttg gagttatacc
    10501 gttccttctg atgaacgcag attttacagg tctgctgctg tccgcaatcc ttaatgatcc
    10561 ggggtttaca aggttttggg gttgagaagg tttagagggt tttgaaggtt gagaaagttt
    10621 agatggttgt gagggttgag agggttgaga aggtttagaa ggtttagagt gtagaga
//
LOCUS       DAOOZV010000061        10231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6354_length_10231_cov_62.831958, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000061 DAOOZV010000000
VERSION     DAOOZV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6354_length_10231_cov_62.831958"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2116)
                     /locus_tag="PLW08_06505"
     CDS             complement(200..2116)
                     /locus_tag="PLW08_06505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HPL52286.1"
                     /translation="MQETTLNSYQIIKTVHSSLRFSLYQAKQMETGQIVLLKTPDTQR
                     LDDEKLKQDLMNEANTHLKLSHPQIRKVYEIREEKGTAYLIGEYIEGMSLSAYLKINS
                     DSLPIELTLSWLKELLEALSYAHKQECIHLNLNPYNLIVDNANAMHIIGFGKNQDAYK
                     TANEKNGTYHPLLYLPPELFQAGIALPQSDLYSFAVIAYQILCGVVPWRIDLQLSLEK
                     QKQQSLCRAVIMPEILHKQVPDWLFTALLQCLKLDPHQRFANAEELITVFNEQENWLP
                     KEDEKVESVQKVEKIESIEEVEKIEKVEKVEETEKDELPNSRAESSKTEFATPIYPAP
                     ESFITEIKETKKLKKTFKILIWLSLIIILFIIVKYIAFDNRPKFTSVADSTQVEISEE
                     PLNENLPIAMVLVPADTLVMGNISPEAEDDEFPLITIGIPAFYISKKEISQAEWMMVF
                     PNNPAHSKDPELPVENVNFYDIIDFCNQKSILDGFEPCYDYYDDEVVCNFSANGYRLP
                     TEAEWEFAAKGGKRNDFFVYSGSDKADEVGWYNENSDAQSHPSGQKKPNQLGIYDLSG
                     NIFEWVWNWYVPYSSRNYDPYKGPDKGIDKVIRGGSWYHNASEMRVTNRNFAKPYTKN
                     AYLGFRVVRTYSPR"
     gene            complement(2117..3370)
                     /gene="hutI"
                     /locus_tag="PLW08_06510"
     CDS             complement(2117..3370)
                     /gene="hutI"
                     /locus_tag="PLW08_06510"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPL52287.1"
                     /translation="MNADVIIINISQLVTLANNSKPRYGKEMEESVIITDAGIAIKGG
                     NIIEINDSKAIGNKYLTSEIIDAEGKIATPGFVDCHTHPVFVHTREDEFAMRLQGKSY
                     VEIAKAGGGILSTIQTTRNADEDLLFELAKKRILKMMQLGTTTLEAKSGYGLNTPSEL
                     KQLRVIKRLQEELPIDIVPTFLGAHEFPPEYRNNHSGYVELLCKEMIPAVAEQGIAEF
                     CDIFTEAHTFNIEESRQILTCAGEHGLKLKMHCDEIEPIGGAELAAELKCISADHLGS
                     ASETGIKALKKAGVIPVLLPATLFSLQSKTYANARLMMDLGLPVAIATDFNPGSCNCD
                     SMQFTMSLACLQMGLNPSSALCAATLNSAYALDRGNQIGSLEAGKKADIVLWDIPGIN
                     FIPYHLGSSYVDRVLKCGKTIYKAT"
     gene            3997..5316
                     /locus_tag="PLW08_06515"
     CDS             3997..5316
                     /locus_tag="PLW08_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPL52288.1"
                     /translation="MLRGNEIKPAEGKLGVMLVGLGAVSTTFVAGIQAIKKGISEPFG
                     SVTQMSTIRLGKRTENRTPLIKDFVPLANLEDLEFMAWDIFEYDAYEAALKAGVLEKE
                     LLDKIEPELRSLKPQKAVFNNNYVKKLHGTYVKTGINKMDLAQQLMDDIKRFQEKNNC
                     SRLVMAWAASTEVYLESDPVHNTLESFEQGLRDNHPAIAPSMIYAYAALQMGIPFLNG
                     APNLNLEIPALRKLAEEKKVPVAGKDFKTGQTLMKTIIAPGLKARMLGIHGWFSTNIL
                     GNRDGEVLDDPESFKTKEVSKLSVLETILEPEKYPQLYKNLYHKVRINYYPPRGDNKE
                     SWDNIDIFGWLGYPMQIKINFLCRDSILAAPVLLDLILLIDLAQRCNMSGVQEWLSFF
                     WKSPMTAPGLYPENDLFIQQMKMKNTLRYMMKEDLITHLGLEYYNEM"
     gene            5378..6484
                     /locus_tag="PLW08_06520"
     CDS             5378..6484
                     /locus_tag="PLW08_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPL52289.1"
                     /translation="MKKIKLLFRIGYAYHKSAFDPIIDLLLNNDKYDVWFSLDMEKIK
                     YFIFEFPYRNQIIEDWKKLGYRFTNETKGFDIVISGDTLRNAKDYGKTLLIFLNHGTG
                     IKNILYRNLARSPGVKYQIFVEGQHRVDSLLKCPYLGKSEVHLIGLPKLDYYFQGKFN
                     REEVLQRWGLNPAKKTVLFAPTYKPTCMYEIKDDIFEQTRDYNLIIKLHPYSWMGKYA
                     PHKQHRIFEKRVKKYPHSVLLPFTEFNIVPYYAAADTVLSEASSTVFDFIALNKFGIV
                     YDLACDKLNHSDGQPLLEIDNREFLKGAYPHIQNGKQLPEAVATALNPTLDMIAKADE
                     YRQRYFYGLDGKAAIRFVEKMEELYYEGGHENGV"
     gene            6488..7207
                     /locus_tag="PLW08_06525"
     CDS             6488..7207
                     /locus_tag="PLW08_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPL52290.1"
                     /translation="MKGVIISAGIGSRLWKTTKQVPKTLLPYKNGTILSTIIEQLKQA
                     GITELIIVVGFKKNYIVDYLQKNPPSLPVTFVENLEWEKGNALSVYKVKNYIIAEPFL
                     LSMADHLVKVEALKQIVDSPERINLLLVDPFITENFDLDDATKVKTENDYIIAIGKEL
                     TDYNALDCGIFRLEPDFFSAVEKAVAKGKDSISNAIAELIPLKRIKSITLNKANQWLD
                     IDTPEAYQFAQHFDLGETTSL"
     gene            complement(7318..8052)
                     /locus_tag="PLW08_06530"
     CDS             complement(7318..8052)
                     /locus_tag="PLW08_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPL52291.1"
                     /translation="MNKADFYRTELLVGQQGMQKLARTCVAIIGLGGVGSYSAEALAR
                     SGIGKFILIDFDVIEATNINRQILALHSTIGKPKVEIMQQRILDINPQADVIIYKEFL
                     DKENQERLLTGADYCVDAIDSLDSKICLLEYLIKNEGKFISVMGAGNRLDPFQVHLST
                     IDKSYNCPLARRVRKLLSKRGVQRNFPCVYSSELPLVPEKNNDISEEGVIDRAKQKQV
                     IGSISYLPAVMGLMAASYVIRDILKD"
     gene            complement(8045..8770)
                     /locus_tag="PLW08_06535"
     CDS             complement(8045..8770)
                     /locus_tag="PLW08_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPL52292.1"
                     /translation="MAFIIDAHCHLANLDVLMPVKPLMDETKKFGLDFWLSSALTKAE
                     IAWHQKNPDKRIAYSAGIHPNYKECDLELKDIEELCIEKKIWAIGEIGLDRNNPDFAG
                     QLKIFIEQMELAKQYNLPVVLHLVGHISEAYKLLKQYKLPYLVHGYAGSLQGFKELLR
                     LKSFFTISERLLKWDKKELLRAMIDSKSYLFETDITRDYVQEEETNPLLRLNTLISSV
                     AEFAEIPRGKLIEQQQKNFNSYE"
     gene            complement(8780..9799)
                     /gene="waaF"
                     /locus_tag="PLW08_06540"
     CDS             complement(8780..9799)
                     /gene="waaF"
                     /locus_tag="PLW08_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPL52293.1"
                     /translation="MKEPQNILIVQTAFIGDVILITPLIRATAELYPQAKIDVIVVSE
                     AVKLLENNPYVNEVIIDEKRKNVVTSTLQLIKQIKSKHYDLVISPHSSFRTHLILYLS
                     KIPERIGFNRGSAAWMLTKKVEHPVGPHKIVKNLGLLKTISEREFDLQTELFPSEKDR
                     QKAEKLLQPLSGKTIIAVAPGSIWQTKCWEPNSYISLCRKLIDSGYAIVLIGGESDKL
                     ISNEIENALGKNNTNLINLAGVTNLLQSAAVIAKCSLMICNDSGAMHIANAMQTRVFA
                     FFGPTVQRFGYYPYREGDRVFEVELECRPCGSHGSKKCPQKHHNCMKQIEVEPVFKAV
                     ETTLR"
     gene            complement(9796..>10231)
                     /locus_tag="PLW08_06545"
     CDS             complement(9796..>10231)
                     /locus_tag="PLW08_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL52294.1"
                     /translation="NLFFMAVGGGDLLSEMKNLAQELGLQGHIAFTGFQKEVGHFLKA
                     FDIFVLASYLEGLGTSVLEAMSVGLPVIGTKAGGITEMIISGKNGLLVPPQNPSELSK
                     AILYLAQNPLLREEYGKKALESVQNFDKERMIAKYLELYKSL"
BASE COUNT         3133 a         2049 c         1826 g         3223 t
ORIGIN      
        1 ttaagaatga gttccgtagg aacgacagat actaacatcg ggtttcaacc cggtggtaaa
       61 aatcccttta gaaatcttca gtttcttgtg taaatcagaa tttgccatcc cgtttttgcc
      121 aactgtatta tttgtgttta tcatataaaa aagtaaaaat ccctaccaaa ttagccaggc
      181 agaatataat gattatgtat taacgagggc tataagttct tacaacgcgg aagcccagat
      241 aggcattttt agtgtagggc ttggcaaaat tgcggttagt aactctcatt tcagaagcat
      301 tatgatacca ggaaccgcct ctaataactt tatcaattcc tttatcgggt cccttataag
      361 ggtcataatt gcgtgaactg taaggcacat accagttcca cacccattca aaaatgtttc
      421 cgctcagatc gtaaattccc agctgattag gttttttttg tcccgaagga tgactttgag
      481 catcgctatt ttcgttatac cacccgactt catctgcctt atcagagcca ctatagacaa
      541 agaaatcatt tctttttccg cctttggcag caaattccca ttcagcttca gtaggtaaac
      601 gatagccatt agcagaaaaa ttgcacacaa cttcatcatc ataataatcg tagcagggct
      661 caaaaccgtc cagaatactt ttctgattgc agaaatcaat aatgtcgtag aaattaacat
      721 tttccacagg cagttcggga tctttactgt gagccggatt attaggaaaa accatcatcc
      781 attctgcctg gctgatttcc tttttactga tgtaaaaagc aggaatacca atggttataa
      841 gaggaaattc atcatcttcc gcttccggag aaatatttcc catcactaaa gtatctgcag
      901 gcaccaaaac catcgctata ggcagatttt cgtttagagg ttcttccgaa atttccactt
      961 gagtgctatc tgctacggaa gtaaattttg gtctgttgtc aaaagcaatg tattttacaa
     1021 taataaacag aattatgata agagacagcc agataagaat tttgaaggtt tttttcagct
     1081 tttttgtttc ttttatttct gtaatgaaag attcgggagc aggatatatg ggagtggcaa
     1141 attctgtttt agagctttcc gctcgggaat ttggcaattc atctttttct gtttcctcaa
     1201 ccttctcaac tttttcaatt ttctcaacct cttcaatgct ctcaattttc tcaactttct
     1261 gaacgctttc caccttttca tcttctttcg gcagccaatt ttcctgttcg ttaaataccg
     1321 ttataagttc ttccgcattg gcaaagcgtt gatgaggatc aagtttcaaa cattgcagta
     1381 aagcggtaaa aagccagtcc ggaacctgtt tatgcaaaat ttccggcata ataacagccc
     1441 ggcaaagtga ctgctgtttt tgtttttcca ggctcagttg caggtcaatt ctccagggaa
     1501 caactccaca aagaatttga taagcgatta cagcaaaaga atatagatcg gattgaggaa
     1561 gagcaattcc tgcctgaaaa agttccggag gaaggtataa aagaggatga taggtgccat
     1621 ttttttcatt agctgtttta taggcatcct ggtttttccc gaaaccgatt atgtgcatag
     1681 cgttagcatt atcaacaata agattataag gatttaaatt caaatggata cattcctgtt
     1741 tatgggcata ggaaagtgcc tctaaaagct cctttagcca ggaaagggta agttcaatgg
     1801 gtagggaatc ggaatttatt ttcagatagg cagaaagaga cattccttca atgtattctc
     1861 caatcaaata agcagttccc ttttcttctc tgatttcata gacctttcta atttgaggat
     1921 ggctaagttt aagatgggta ttggcttcat tcatcaaatc ctgtttcagt ttctcatcgt
     1981 caagtcgttg cgtatcagga gttttaagca aaacaatttg tccggtttcc atttgttttg
     2041 cttgatagag agaaaacctc aaggagctgt gaacggtttt aattatctga tagctgttta
     2101 gagttgtctc ctgcatttaa gttgccttat aaattgtctt tccgcatttt agtacgcggt
     2161 caacataaga agaacctaaa tgatagggaa taaaattaat ccccggaata tcccaaagaa
     2221 caatatctgc cttttttcct gcctccaaag agccgatttg attgcctcta tccagcgcat
     2281 aagcagaatt taaagtggct gcacataaag cagaggaagg atttaacccc atttgcaaac
     2341 aggcaagaga catagtaaac tgcatactgt cacagttaca acttcctgga ttaaagtccg
     2401 tagctatggc aacaggaagt cccaaatcca tcatcagacg ggcattagcg taggtcttgc
     2461 tctgtaagga aaataaagtt gccggtaata aaacaggtat cactcccgct tttttgagcg
     2521 ctttaattcc tgtttcgctg gcagaaccca aatggtctgc tgatatacat tttagttccg
     2581 ctgcaagttc tgcaccacca atgggctcaa tctcatcaca atgcattttt aatttgagtc
     2641 cgtgttcacc tgcacaggta agtatttggc gcgattcctc aatattaaaa gtatgcgctt
     2701 ctgtaaaaat atcgcaaaat tcagctattc cttgttccgc gacagcagga atcatttctt
     2761 tacataggag ttctacatag ccggaatgat tatttctata ttcgggagga aattcatgtg
     2821 cacctaaaaa agtagggaca atatcaatcg ggagttcttc ctgcaagcgt ttgatcaccc
     2881 gtaattgttt caattcgctt ggggtgttca gtccgtaacc gctttttgcc tcaagggttg
     2941 tagttcctaa ctgcatcatt ttcaggatgc gttttttagc caattcaaaa agcaaatcct
     3001 catctgcatt tctggttgtc tgaattgtgc ttaaaatgcc acctcctgct ttggctattt
     3061 ccacataact ttttccctgt aaacgcatag caaattcatc ttcgcgagta tggacaaaaa
     3121 cgggatgagt atgacaatcc acaaaaccgg gagtagcaat tttcccttcg gcatctataa
     3181 tttcggaagt tagatatttg tttcctatcg ctttactgtc gtttatttca ataatattgc
     3241 caccttttat agctatcccc gcatcagtaa taataacact ttcttccatc tctttgccgt
     3301 agcgtggttt actattattg gcaagagtaa ctaactggct gatattaatg ataatcacat
     3361 cggcgttcat tctttcctca tactttagca atcttaaaaa tacattatgt aattcctaaa
     3421 atattccgtc aagctttttc agcagggagc attgcttacc cattgcttac ccattacgga
     3481 tggcattagc aatgggtaag caatgggtaa gtaatgctcc taaggaagaa cttctatttt
     3541 acatattttc aggatgttat gcgtttactc acttccctgt gttttcccct gaaattattt
     3601 tctgaaagtc agagctaatc actgccgaaa taattgcaag tcaggagtcg tctctgcttt
     3661 caaaaatttt acacctccca cacattgatt tggcagtgag gacttatgac gaccctaaaa
     3721 aatataataa tagaacgcag aaaactggat caaccggatc agcaggattt aatatctgta
     3781 ataaaaaaat aaaaaaatct gcgtaatcag cgcaatttgc gtgaagttta tttggttgaa
     3841 taggacttca accttccaga aaaacactgt tctccaacaa cactttcgtt attttaggta
     3901 acttcagtgt tcttggtgta aaataaacat ttatgcttga cacctatgca tttatatatt
     3961 tactggataa aaataagcaa caaaaaggga gaaaaaatgc ttagaggtaa tgaaataaaa
     4021 cctgcagaag gcaaacttgg cgttatgtta gtcggtttag gagctgtaag tacaactttt
     4081 gtagctggca ttcaagcaat taaaaaaggc atttccgagc cctttggttc ggtcactcag
     4141 atgtccacta tccgtttagg caaaagaaca gaaaatagaa ctcctttaat aaaggacttt
     4201 gttcccttag ctaatttgga agatttggaa tttatggctt gggatatttt tgaatacgat
     4261 gcttatgaag ctgctttaaa agcgggagtt ctggaaaaag aactgctgga taaaatagaa
     4321 ccggaattgc gttctctgaa gccacaaaag gcggttttca acaataacta tgtaaaaaaa
     4381 ttacacggca cttatgtgaa aaccggaatc aataaaatgg atttagctca gcaactgatg
     4441 gatgatatca aacgcttcca ggaaaaaaat aattgcagcc gcctggttat ggcttgggct
     4501 gcttctacag aggtctattt agaatcggat cctgttcata atactcttga aagttttgaa
     4561 cagggtttgc gcgataatca tccggcaatt gcaccttcta tgatttatgc ttatgccgct
     4621 ttgcaaatgg gaatcccttt tctgaacgga gctccgaact taaatctgga aattcccgct
     4681 ctgagaaaac tggcggaaga gaaaaaagtt cccgttgcag gtaaagattt caagacaggt
     4741 caaaccctga tgaaaactat aatcgctcca gggcttaaag ccagaatgtt agggattcac
     4801 ggttggttct ctacaaatat tttaggtaat agagacggtg aagtgcttga tgaccccgaa
     4861 agctttaaaa ccaaagaagt tagtaaactt tccgtgctgg aaacaatttt ggaaccggaa
     4921 aaatatcctc aactctataa aaacctttat cataaagtcc gcatcaatta ttatcccccg
     4981 cgtggcgata ataaagaaag ctgggataat atagatattt ttggctggct cggttatccg
     5041 atgcaaataa aaataaattt cctgtgcaga gatagcattc ttgctgctcc tgtattgttg
     5101 gatttaattc tcttaattga tttggcacag cgctgtaata tgtccggagt tcaggaatgg
     5161 ctttctttct tctggaaatc accgatgacg gctcccgggc tctatcctga aaacgatctt
     5221 ttcattcagc agatgaaaat gaaaaatacc ctgcgttata tgatgaaaga agaccttatt
     5281 actcatctgg gtttggagta ttataacgaa atgtaatttt cttgtatggg gcttgtctcc
     5341 atacctattt tgatactttt agcagattaa acagacgatg aagaagataa aactcctgtt
     5401 cagaataggt tatgcctatc ataagtccgc ctttgatccg attatagacc tcttgctgaa
     5461 taacgataaa tacgatgtct ggttctcatt ggatatggaa aaaataaagt atttcatctt
     5521 tgaatttccc tatcggaacc agataattga ggactggaaa aaacttggct accgttttac
     5581 caacgaaacc aagggctttg atatcgtaat ttccggtgac acacttcgta atgcaaagga
     5641 ctatggaaaa acactattga tttttctgaa tcacggaaca ggaattaaaa acatacttta
     5701 tcgcaattta gctcgctctc ctggggttaa ataccagatt tttgtggaag gccaacatag
     5761 agttgattcc ttgttaaaat gcccttattt gggaaaaagc gaagtccatt taatcggctt
     5821 gcccaaattg gactattact ttcagggtaa atttaacaga gaagaggtct tgcaacgctg
     5881 gggtcttaat cccgctaaaa aaacagtcct cttcgctccc acttataagc caacctgtat
     5941 gtatgaaata aaagatgaca tctttgagca aaccagggac tataacttaa taattaaact
     6001 tcacccatac agctggatgg gaaaatatgc cccgcataaa caacatcgca tttttgaaaa
     6061 aagagtgaaa aagtatcccc attccgtgct tttacctttt acagaattta atattgtccc
     6121 ttattatgct gctgccgata cggttctcag tgaggcatcc agcacggttt ttgattttat
     6181 tgctctgaat aaatttggta ttgtttatga ccttgcttgc gataaactta atcattctga
     6241 tggtcaacct ctgttggaaa ttgataaccg ggagttttta aaaggcgctt atccccatat
     6301 ccaaaacgga aaacaacttc ctgaagcagt tgccacagct ctcaatccta ctttagatat
     6361 gattgctaaa gcggatgaat accgtcaacg ctatttctat ggcttggatg gaaaagcagc
     6421 tatccgtttt gtagaaaaaa tggaagaatt atactatgaa ggcggtcacg aaaatggagt
     6481 gtaaattttg aaaggtgtaa taatttctgc cggaattggc agtcgccttt ggaaaactac
     6541 aaagcaagtt cctaaaaccc tgcttcccta taaaaacgga actattcttt ccactattat
     6601 tgaacagcta aaacaggcag gaataacgga actgattata gtagttggtt ttaagaagaa
     6661 ttatatcgta gattatctgc agaaaaatcc cccgtcttta ccagttactt ttgtggaaaa
     6721 ccttgaatgg gaaaaaggca atgctctttc ggtttataaa gtaaagaatt atataatagc
     6781 tgaacctttt ctcctttcta tggctgatca cctggtaaaa gtggaagcat tgaaacaaat
     6841 tgtggattcc cctgaacgca taaacctgct tttagttgac ccctttataa cggaaaactt
     6901 tgatcttgat gacgctacca aagtaaaaac tgaaaatgat tacataattg ccatcggcaa
     6961 agaactgact gattacaatg cccttgattg cggaattttc cgtttggaac cggatttctt
     7021 ttctgcggtt gaaaaggcag ttgccaaagg aaaggactct atcagcaatg ccattgcaga
     7081 attgataccg ttaaagcgca taaagtcaat tactttaaat aaagcaaatc aatggttgga
     7141 tatagacact cctgaagcat atcaatttgc tcagcacttt gatctcggag aaactacatc
     7201 cttgtagttc cacctcctgt agtttaatta gctaccaata attgaatggt aacgaaaaaa
     7261 gcttagttac cagagaacga caagatgttt ttcttctttt ttttatcaga tacaacatca
     7321 atcttttaat atatccctta ttacataaga tgcagccatc aatcccatta cagcaggtaa
     7381 ataactgata gaaccaataa cttgtttttg ctttgctcta tcaataactc cctcttccga
     7441 aatatcatta tttttttcag gaacaagggg caactcggaa gaataaacac agggaaaatt
     7501 tctctgaaca cctctcttgc ttaatagttt tcttactctt cttgctaaag ggcaattgta
     7561 gcttttatca atggtagaaa gatgaacttg aaaagggtct aaacgatttc ccgctcccat
     7621 cacactgata aatttgcctt catttttaat caaatactcc aatagacata ttttggaatc
     7681 caaactatca atggcatcta cacaataatc agctcctgta agtaatcttt cctgattttc
     7741 cttgtctaaa aattccttgt aaataatcac atctgcctgt ggattgatat ccagaatcct
     7801 ctgctgcatt atttccacct tgggcttgcc aattgtggaa tgcaaagcca aaatctgcct
     7861 gttgatatta gttgcctcaa ttacatcaaa gtcaatcaag ataaatttcc ctattccgga
     7921 acgagctaag gcctctgcag aataagaacc aactccaccc aaaccaataa ttgcaacgca
     7981 ggttcgggct aatttctgca ttccttgttg tccaactaat agctctgttc tataaaaatc
     8041 cgctttattc atagctgtta aagttttttt gttgttgttc tattaattta cctcttggaa
     8101 tttctgcaaa ttcagctaca gaagaaataa gagtatttaa gcgcaaaaga ggattagttt
     8161 cttcttcctg cacataatct cgggtaatat ctgtctcaaa aagatagctt ttactatcta
     8221 tcatagctct taaaagctct tttttatccc acttcaataa acgctcggaa atagtaaaaa
     8281 aggactttag gcgtaaaagt tccttaaaac cctgtaagga acctgcataa ccgtgaacaa
     8341 gatagggcaa tttatattgt ttcagcagtt tatatgcttc gctaatatgc cctaccaggt
     8401 gtaaaaccac tggcaaattg tattgtttgg caagttccat ctgctctata aatatcttca
     8461 gttgacctgc aaaatcagga ttgtttctgt ccaagccgat ttctccaatt gcccaaattt
     8521 ttttctctat gcaaagttct tcaatatcct tcagttccaa atcgcactct ttatagttag
     8581 gatgaattcc agcactatag gcaatgcgtt tatcagggtt tttttgatgc caggcaattt
     8641 ccgcttttgt aagtgcagaa gaaagccaaa agtccaagcc aaatttcttt gtttcatcca
     8701 ttaagggttt aaccggcatt agcacatcca aattggctaa atgacaatgc gcatctatta
     8761 taaaagccat cgttctctcc tatctcaaag tagtttctac tgccttaaaa accggctcta
     8821 cttctatctg tttcatacag ttatgatgtt tttgcggaca ttttttactg ccatgactgc
     8881 cacagggtct acattccagt tccacttcaa aaaccctgtc tccttcccga taaggataat
     8941 agccaaaacg ctgaaccgta ggcccaaaaa aggcaaaaac ccttgtctgc atagcattgg
     9001 caatatgcat tgcgccacta tcattacaaa tcattaaaga acattttgca attacagcag
     9061 cactttgcaa taaattagta actcctgcca gatttattag attagtatta tttttcccca
     9121 aagcattttc aatttcatta cttatgagtt tatcgctttc ccctccaatt aaaacaatag
     9181 cataaccact atctatcagt tttcggcaga gtgagatata agaattcggt tcccaacatt
     9241 tggtttgcca gatagaacca ggtgctacag ctattattgt ttttccggat aagggttgaa
     9301 gtaatttttc cgctttctgc ctatcttttt cggaaggaaa aagttctgtt tgcagatcaa
     9361 attctctctc gcttatagtt ttcaaaagtc ctaagttttt aactatctta tgcggaccga
     9421 caggatgttc tacttttttt gtcaacatcc aagcagcaga acctctgtta aaacctatgc
     9481 gttccggaat tttgcttaag taaagaatta aatgagtcct aaaagaacta tgcggagaaa
     9541 taaccaggtc ataatgttta cttttaatct gcttaattag ttgcaaagta gatgtaacca
     9601 catttttacg cttttcatct ataattactt cattaacata aggattattt tccagtaact
     9661 taaccgcctc agaaacaaca attacatcta tttttgcctg gggatacagt tctgctgtag
     9721 ctcttatcaa aggtgtaatt aatatcacat cgccaataaa agctgtttgc actattagaa
     9781 tattttgcgg ctctttcata gtgatttgta tagctccaga tatttagcta tcattcgctc
     9841 tttatcaaaa ttttggacgc tttctaacgc ttttttgcca tattcttctc tcaataaagg
     9901 gttttgcgcc aaatataata ttgctttaga taattctgaa ggattctgag gaggaacaag
     9961 aagaccattt ttcccagaaa taatcatttc cgtaatacct cctgcttttg ttccaattac
    10021 aggaaggcct acactcattg cttctaaaac ggaagtacca agcccttcca gataggaagc
    10081 caaaacaaag atatcaaatg cctttaagaa atgtcccact tctttttgaa aaccggtgaa
    10141 cgcaatatgt ccctgcaaac ctaattcttg agcaagattt ttcatttcgg aaagtaaatc
    10201 tcctccacca acagccataa aaaacaggtt t
//
LOCUS       DAOOZV010000062        10226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6360_length_10226_cov_61.369678, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000062 DAOOZV010000000
VERSION     DAOOZV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_10226_cov_61.369678"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(645..2795)
                     /locus_tag="PLW08_06550"
     CDS             complement(645..2795)
                     /locus_tag="PLW08_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPL52295.1"
                     /translation="MQVLRFFTVFFILLAFFAMLNGIKVTGIVTDEENKPLEAVRLIS
                     GKKTTLSKQNGNFTIEVTDSLQITRLGYKKQVLSIQEVIALPKTTSGIQIRLQSEPVQ
                     LSTYRVFAYLSEENISPADVVTLPIDPDKHYSSASELISQTASFQGSGIQLKGEIAEI
                     NILGNISRHTLVLLDGIAMNTLGEPFDFSRLNLDNVESIEVVKNNASVYGGGSAIGGI
                     IMITTKQGAALKTNLESKTEFGSYGYLLQQISLSGISSQNSYRLCLNAYNADNDFPIP
                     KGDLIPEDSENKRKNNSKQQLSFSGAYSTHLSKLLMSISTDYLTFQRELPGPLNFADL
                     YYKAFLKGSSLRPQIRVSANFGNFTWQSNNWLLKDETVYDNTQSLIEGFSQKYKQKND
                     NYGIKQSLSYNKNSLQASLSGEYVYNRYRYQDLINPKQSNIDYTRNQAAVSLKLNQQK
                     EFAWLILNNGLAGRYDYIDGEDEFSGRLELSLRNWGTKYLETGATIGNSFALPTPYDL
                     YWKGDSQTLGNPNLKSEKATGYQIWLKGNIPQATLKATLHQNTIKNLIQWRQVQMYGP
                     VWKPMNIGRAEIRNLELEGNYQPLKKLELTASAVFTKAKDTTYYSFAEAPKLMYRPET
                     LYSLAVDYQPGIFHFWTKYSYTGKQWITPDNLIDPISAYALVDCGISLHWQKGNWKFT
                     PAFTVKNLTNENYMVHAYVPEPGRTFYATLQLNR"
     gene            complement(2806..3573)
                     /locus_tag="PLW08_06555"
     CDS             complement(2806..3573)
                     /locus_tag="PLW08_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL52296.1"
                     /translation="MIVLKNVTCGYQDFPVLQDISLQIESGEFCALLGPNGAGKSTLL
                     YTIMGYLKPSKGFISIFGKEIASVKHSWLAKQLAFVPQETRSEFDHTVQETVLMGRYP
                     YLGLLQSYSSEDFDTVDSVLEELKLLELKHRWLSEISGGEKQRVLIARALVQQTPFIL
                     LDESLSQLDINYQIEIMKLLKAIHSKENKTVLIVSHNLNLAANYAERLIFLKEGKVLA
                     SGTPELLMQKETLKELFTIELDIMQNPHSGRPNIIYP"
     gene            complement(3806..5488)
                     /locus_tag="PLW08_06560"
     CDS             complement(3806..5488)
                     /locus_tag="PLW08_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52297.1"
                     /translation="MPKYLILTMLLFSLSVLTAGDYIIGTGTGNQYYVPFYGGNNYGW
                     SRFFFTADELLAAGMTGTVQITKIGFQLVSNTLDNYITNDQRIYIREFYDENYPSNPQ
                     YINPSSYPVAYQGTISWTSPGWVFIQLTTPYSYTYNGGSPLPNGIEILWENRDGSSQS
                     PYPKFAYTSTDENRAAYKYSNSSFPTGSGTRYNKRPNVWFISPATSEPNPAVNPIPAN
                     LATGVEITTTLKWTSGGGNPDDYLVSLYKTDPLTYIVNNQVTTSTTYNLPVRLEYSTT
                     YYWRVIPRNSFGSAIACPTWSFTTIPDPSIMTYPWTENFDGTSFPPNSNWLRRGGDLV
                     DPIQLGGSSLWEQDDWLNIPRTDKAARINIWGNVNGWLITPQFLFNEDSDYLYFDLAL
                     LKYNQLPNGTPPDTTGIDDRFAVLISDGYTWSTANIMREWNNSGSPYVLNNISPWGER
                     VCIPLNGLTGHKRIAFFAGSTISNADNDFMINNLYVGPLIPDTPEVQISQVGDDLCLL
                     TWNESSGATGYDIYAADSPEGPWELIASTAETQFEFTAQAAKKFYRVKAIAR"
     gene            complement(5499..8759)
                     /locus_tag="PLW08_06565"
     CDS             complement(5499..8759)
                     /locus_tag="PLW08_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPL52298.1"
                     /translation="MKKSFLLCLLICCSAFAFGQWHIDEDFEGITTLPAGWTVYDDGD
                     GMTWRNLENASHAHSGTRFAFADNYFPNQNCDWLITPQLHIANGDSLKFYTRAWFDTE
                     NLQVYVSTTGNAISNFNTQILNLQGLGTAFQYASYNLSAYAGQNIYIGFKWQCVTYGI
                     IVDDVKIGQPVIVTPELNLPESITFWQGETITVDFAPYIVATDINNIQLTWQTPEHIS
                     ISATGLALTFSSDNWNGTENITFTLTDNITGLTATDTIQIIVHPIPTVDMALQTILSP
                     QNNEYVNSAFSPVVVVKNNGQILWEDQLEVCFKVYNSQNVLVESVASYFNPVLQPEAT
                     FEAVFPPLSISSEGVYTGTFQINISDNNPTNDTISCVFNVILRVNTGGPDAFGYRYID
                     STSENGPDFNWIDISATGTSTIMYGVNAFNGDDNFSEPIPLGFNFPFYGISYSTAYVD
                     INGEILLAPNTWYTEYPDNGWGNDGNMFNYMYPIPGYSAMPGLIAVYWDDLHCDQGTG
                     DIYFQSFGEEPNRYCVIQWHNLRFHAGTGVSTLLDFEVILQENGEIVMQYNSTATGQT
                     GANVPHDNGKSATVALQNADATMGICYLREIVQNGNYLGVEPAGNLLFNGLALRFYSG
                     EDTQAPFITHSAPGNTFNQSPLITARILDLSELNNVTLHYNFGEGWNALEGIETGDGN
                     YDFALPLLPMGHTFSYYFAAADVCGNSSTLPAGAPIEVFQFKILPSGNTSVLLAYSGK
                     QDYQNTELPVYIEQLNNANIAYDIYDWEEFAEYTIPNVYSTILAYACTGTASPATLYL
                     SQKLMDFLNSGTIDNPKNLFFSSDGFAFSQSGTPNSHPMKQLLNGYFRTFAVSTGGGG
                     GTNGLAGPDVFSYQSGTIMCTTYSPVGTIGTEYSVYANSPDCIFAYDEVPDWYADLVP
                     YPEIGAMNAFVFEDGPVEGHAYLYHGVCATAVQTPIFRTFYFSFDFSQLNNPAQREEL
                     FADLMDWFEVYPTSINENELPIVQSKLQTNYPNPFNPATIIAFTLAKADKVEIVIYNL
                     KGQKVKTLLSDNLLAGNHSIVWDGKDDKGKTLSSGIYLVKMKTGKTTDTRKITMLK"
     gene            complement(8775..10142)
                     /locus_tag="PLW08_06570"
     CDS             complement(8775..10142)
                     /locus_tag="PLW08_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL52299.1"
                     /translation="MIQNLQLIRNQAQLKEMGSPLKYNILKELIKAPATCQQLATLFG
                     CSKQKMHYNLTQMLSQGLLKIEDEANINNKEVYYRATARSYVLDLAIGLETSEKILDN
                     RNIINSILEQEYRINLNNIADKLLDEALKLTKGMHLLITTGKFNLPLVEKLLLEAGKR
                     GIYTTLIYQDLEQLQARAEQYSLIAFQADYENFNHKLKEADVYLNLNGESRIVEVTDP
                     KKIQIRNRMLEKGRKIIKEKNIRLAMMPSLLNDTLSEQAIESEVQFWRALDIDYQMLS
                     DKTLALCRKFVNKNYVEIENKSAAFRFAINNIWAECGSFGKGEFQSPIINLPGGEILI
                     IPKPGSMQGKIIGDRAYALGEEILHPVVEIVNNEITGFSAESNQQQLAKAIETGGKDG
                     RKVALICLGTNDNIRGGNIDNALKHKSSGFISIYWGSNKDIGGDIAGNIEWFIQIENP
                     QLNFL"
BASE COUNT         3088 a         2059 c         1945 g         3134 t
ORIGIN      
        1 taaagctaat aatctatcgt ttaacagacc ttaatcaagc gttaattatt aacgcttgat
       61 taaggccaga ttaacacttg atttatgctg tcaagtaggg gcaaaaaaag aagcttgaat
      121 ttgataagca gtttatacta aagtaataaa tgcctgctga caggtaaata aattaggata
      181 atacaaaaaa atcaacccga aattttacca tttagggaaa atcgggtggt aaaggtaaaa
      241 agtattctct ctcagataca tataattgtc agctataatt ggaagaatca caggaatagg
      301 tttatcggta ttcaattcgg attggaagat ttattcaggg ttttgattat ttctacctgt
      361 ctcaataaaa cctgatgata taattattta tttttgcttt aaccagtttt tgcttagtgc
      421 catttataat ctctggtagg ggggtactta taccctttac tagccatttt ttctaaaact
      481 tgcatccagc caaaaatatt gtagctactt tccccaatga taatctactc aaataactta
      541 tggaaaaata tagcatttcg cttaatagca aatattaaag tagtgcccgg ttgtcagatg
      601 cataaatctt cctcagtttc tataaagcag ttgctgataa gaagttatct gttcagttgc
      661 aaagtggcat aaaaagttct acccggttca ggcacataag cgtgcaccat ataattttcg
      721 ttggtaagat ttttgacagt gaaagcaggg gtaaatttcc agttgccttt ttgccaatgc
      781 aagcttattc cgcaatctac taaagcataa gcagaaatgg ggtctattaa gttatcgggg
      841 gtaatccatt gtttacctgt gtagctgtat tttgtccaaa agtgaaagat tcccggttga
      901 taatcaaccg ctaaagaata gagggtttcg ggacggtaca taagtttggg tgcttctgca
      961 aaactataat aggtagtatc cttggctttc gtaaaaaccg ctgaagccgt aagttccaat
     1021 tttttaagcg gttggtaatt tccttccagc tccagattac gaatttccgc tctaccaata
     1081 ttcatcggct tccaaacggg accatacatt tgcacttgtc gccattgaat caggttttta
     1141 atcgtatttt ggtgcaaggt tgcctttaga gttgcttggg gaatattgcc tttcagccaa
     1201 atttgatagc cggttgcttt ttcacttttg aggttgggat tacccaaggt ttgagaatcc
     1261 cctttccagt aaaggtcata aggagttggc agagcaaaag aattgcctat tgtagctccc
     1321 gtttccagat attttgttcc ccaattgcgc agggacaatt ccagacgacc gctaaattca
     1381 tcttcaccat caatgtaatc ataacgaccc gccaaaccgt tattcaaaat cagccaggca
     1441 aattcctttt gctgatttag ttttaaacta actgctgctt gatttctggt ataatcaata
     1501 ttgctttgtt tagggttaat taaatcctga tagcgataac ggttatacac atattccccg
     1561 gataaagatg cctgcaaaga gtttttatta tagctgagag attgttttat tccgtagtta
     1621 tcattttttt gcttgtattt ttgggaaaag ccctcaatta gtgactgtgt attatcataa
     1681 accgtttcgt ctttcagcag ccaattattg ctttgccagg taaaattgcc gaaattggca
     1741 gaaacccgaa tttgcggacg cagggatgaa ccctttaaaa atgccttgta atataaatcg
     1801 gcaaaattaa ggggaccggg aagttctctc tgaaaggtta aataatcggt agaaatgctc
     1861 atcagcaatt tagatagatg agtggaatac gcacccgaaa aagaaagttg ctgtttgctg
     1921 ttgtttttgc gtttgttttc gctatcctca ggaatcaaat cccctttggg aataggaaaa
     1981 tcgttatctg cattataggc atttaggcat aaacgatagg aattttgaga actgattccg
     2041 cttaaggaaa tttgttgcag caaatagcca taagaaccga attccgtttt gctttccaga
     2101 tttgttttta aagcagcacc ctgtttagta gtaatcatta ttattccacc aatagcagaa
     2161 ccgcctccat aaacactggc attatttttt acgacctcta tgctttctac attatccaaa
     2221 ttcaaacggg aaaaatcaaa gggctcacct aaagtattca tagcaattcc atccagcaaa
     2281 actaaagtgt gtctgctaat atttcccaaa atgtttattt ccgctatttc acctttcagt
     2341 tgaataccgg aaccttgaaa ggaagcagtt tgcgagatta attctgaagc actgctataa
     2401 tgtttatcgg ggtcaattgg caaagttaca acatccgcag gagaaatatt ttcttcacta
     2461 agataagcaa aaacacgata tgtagatagc tgaaccggtt cgctttgtaa tctaatctgt
     2521 attcctgaag ttgttttagg tagagcaata acttcctgta tagacagcac ctgcttttta
     2581 tagcccaaac gggttatctg taaagagtcg gtaacttcaa tggtaaaatt gccgttctgt
     2641 ttacttaagg tggttttttt tccgctaatc aggcgaaccg cttccagggg tttattttct
     2701 tcatccgtaa caattccggt tacttttatt ccatttagca ttgcaaaaaa ggcaagcaga
     2761 ataaagaaaa cggtaaaaaa acggagaacc tgcataataa aacactcaag gataaataat
     2821 attagggcgt ccgctatgcg gattttgcat tatatccaat tctattgtaa ataactcttt
     2881 cagggtctct ttctgcatta aaagttccgg tgttccggaa gccagaacct tgccctcttt
     2941 caggaaaatt aacctttcgg catagttggc tgccaagttt aagttatgcg aaacgattaa
     3001 aaccgtctta ttttctttgc tgtgaatggc ttttagcagt ttcattattt ctatctgata
     3061 attaatatcc aattgggaca aactttcgtc cagcaaaata aacggtgtct gctgaactaa
     3121 tgctctggca attaaaaccc tctgtttttc acctccgctg atttcagaaa gccagcggtg
     3181 tttcagctcc aatagtttca gctcttccaa aacagaatca acggtatcaa aatcctcgga
     3241 actataggac tgcaataaac ccagataagg ataacgaccc attaaaaccg tttcctgaac
     3301 tgtatggtca aattcagacc tggtttcttg aggcacaaaa gccagttgtt ttgccaacca
     3361 ggagtgtttt acgcttgcta tctccttgcc gaatatggaa ataaaaccct ttgacggttt
     3421 caaataaccc ataatggtat aaagtaaggt agatttcccg gctccgttag gacctaatag
     3481 agcacaaaat tccccgcttt caatttgcag ggaaatatct tgcaaaacag gaaaatcctg
     3541 ataaccgcag gttacattct ttaaaacaat catcgtgcta ataatccgta taatgctatt
     3601 cttctttgaa caggattttt attggaagcc aaattgtcaa cttaactttc accgttttca
     3661 accgggtaat tgacctcccg gtcaactaca caggaggtta acctccaggt caacctcctg
     3721 ttgcgtttta ttactttgcg gagaaacagt gtcctccgcc tacacttttt ttactttaag
     3781 agaaacgatt ggaatatcgt ttactctatc ttgcgattgc tttcacccga tagaattttt
     3841 tagctgcctg ggctgtaaac tcaaattgcg tctctgctgt ggaagcaatt agctcccaag
     3901 gaccttcagg tgaatcagca gcatagatat cataaccggt agctcctgaa ctttcattcc
     3961 aggttagaag acaaagatca tctccaactt ggctaatttg cacttctggt gtatcgggaa
     4021 ttaaaggacc cacataaagg ttattgatca taaaatcgtt atcggcatta gaaattgtag
     4081 agccggcaaa aaaggcaatt cttttatgtc cggttaaacc gtttaaggga atacaaaccc
     4141 tttctcccca agggctaata ttatttaaca cataaggaga accgctatta ttccattcgc
     4201 gcataatatt ggctgtgctc caagtgtagc cgtcggaaat taaaacggca aaacgatcat
     4261 caataccggt tgtatccgga ggagttccat tcgggagttg attatatttc agcaatgcca
     4321 aatcaaagta aagataatca ctatcttcat taaacagaaa ttgaggtgtg atcagccaac
     4381 cgtttacatt gccccaaata ttgattctgg ctgccttatc cgtcctggga atattaagcc
     4441 agtcatcctg ttcccataaa gaactacctc caagctggat agggtcaaca agatcacctc
     4501 ctctgcgtaa ccagttggaa tttgggggaa aagaagtccc gtcaaaattc tccgtccagg
     4561 gataagtcat tattgaaggg tctggaatag tagtaaaact ccaggtagga caagctatag
     4621 ctgatccaaa actattacgg gggataactc tccagtagta agttgtacta tattccagac
     4681 gcacaggtaa attataggta gtggaggtag ttacctgatt atttacaatg tatgttagcg
     4741 gatcggtttt atacaaggaa acaagataat cgtccggatt acctcctccg ctggtccatt
     4801 tcagggttgt agttatctcc actcccgtag ccaaatttgc cggaatagga ttaactgccg
     4861 gattgggctc tgatgtagca ggagaaataa accagacatt aggtcttttg ttatatctgg
     4921 taccggaacc ggttgggaaa gaagaattgc tatatttata ggcggctcta ttttcatcag
     4981 tggaagtata agcgaatttc ggataggggc tttgagaaga gccgtctcta ttttcccaga
     5041 gaatttcaat tccattgggt aaagggctcc cgccgttata agtataagaa tagggtgtcg
     5101 ttagctgaat aaaaacccaa ccgggacttg tccaggaaat agttccttga taagcaaccg
     5161 gataagagga gggattaata tattgaggat tggaaggata attttcgtca taaaactcgc
     5221 ggatataaat ccgttggtcg ttggtaatat aattatcaag ggtgttagaa acgagctgaa
     5281 aaccgatttt ggttatttgt acggttcccg tcattccggc agctaataat tcatccgccg
     5341 tgaaaaagaa cctgctccag ccatagttat taccgccata aaaaggaaca taatactgat
     5401 tacctgtccc agtgccaata atgtaatctc ctgctgtgag aacagacaga gaaaacagca
     5461 gcatagttag aattagatac ttgggcattt tttactcctt atttgagcat cgtgattttg
     5521 cgggtgtctg tggttttccc ggttttcatt ttcaccagat aaattccgct gcttaaagtt
     5581 ttgcctttat cgtctttacc gtcccaaaca atactatggt tacccgcaag cagattatcg
     5641 gaaagcaggg tttttacttt ttgtcccttt aggttataga taactatttc cactttatcc
     5701 gcttttgcca gagtaaaagc tataatcgtt gcgggattaa atggattcgg atagtttgtt
     5761 tgaagtttgc tttgcacaat cggcagttca ttttcgttaa tggaagtagg ataaacctca
     5821 aaccagtcca ttaaatcggc aaacagttct tctctttgag caggattatt cagctgggaa
     5881 aaatcaaagg aaaagtaaaa agtcctaaat ataggtgttt gcacagcagt agcacaaacc
     5941 ccgtgatata aataggcatg accttctaca ggtccgtctt caaacacaaa agcattcatc
     6001 gctccgattt caggataggg aacaaggtct gcataccaat ccggaacttc atcataggca
     6061 aaaatacaat ccgggctatt ggcataaaca ctgtattctg tgccgatagt tcccacagga
     6121 gaataagttg tgcacataat tgtgcctgat tgatagctga aaacatcagg tcctgctaaa
     6181 ccattagttc cgcctccacc acctgtagaa acagcaaaag ttcggaaata accatttagc
     6241 aattgcttca taggatggga attaggagta cccgattggc tgaaagcaaa gccatcggag
     6301 gagaaaaaca ggtttttagg attatctata gtgccggaat tcagaaagtc catcaatttc
     6361 tgtgataaat acaaggtagc aggacttgct gttcctgtgc aggcataagc cagaatagta
     6421 gaatagacat tcggaattgt atattcggca aattcttccc aatcatatat gtcgtaggca
     6481 atattggcat tattgagctg ttctatataa actggcagtt cggtattttg ataatcctgc
     6541 tttccgctgt aggcaagcaa gacagaagta tttcccgaag gtagaatttt gaattgaaag
     6601 acctctattg gagctcctgc aggcaaagta gaactattgc cacagacatc tgcagcagca
     6661 aaataatagc taaaggtatg ccccatagga agtaatggca aagcaaaatc atagttgcca
     6721 tcacctgttt ctataccttc caaagcattc cagccctcac cgaaattata atgcaaagtt
     6781 acattattca gctcggaaag gtctaatatt cttgccgtta tcaaaggtga ctgattaaaa
     6841 gtgtttcccg gagcggaatg agtaataaaa ggcgcttgtg tgtcttcacc gctataaaaa
     6901 cgcagggcta atccattgaa aagtaaattt cccgctggtt caactcccag ataattacca
     6961 ttttgcacaa tttccctcaa ataacaaatt cccattgtgg catcggcatt ttgtaaggca
     7021 acggtagcac tttttccatt atcgtgaggc acatttgctc ctgtttgtcc tgttgctgta
     7081 gaattgtatt gcatcactat ttccccgttt tcctgcagaa taacttcaaa atccagcaga
     7141 gtggaaacac cggttccagc atgaaacctc aaattatgcc attgaattac gcaataacga
     7201 ttgggctctt ctccaaagct ttgaaaatag atatctccag tgccttgatc acaatgcagg
     7261 tcatcccagt aaactgctat caaacctggc attgcagaat aacctggaat cggatacata
     7321 tagttaaaca tattgccatc gtttccccaa ccgttatcag gatattctgt ataccaggta
     7381 tttggtgcca ataatatttc tccgttaata tctacataag cggtagagta gctaatacca
     7441 tagaagggaa aattaaagcc aaggggaatg ggttcggaaa agttatcgtc gccgttaaag
     7501 gcattcactc catacataat tgtagaagtg ccggtagcac taatatcaat ccaattgaaa
     7561 tcaggaccgt tttctgatgt gctatctata tagcgataac caaaagcatc gggtccgcct
     7621 gtatttactc ttaaaataac attgaataca cagctgattg tatcattagt gggattgtta
     7681 tcgctgatgt taatctgaaa agttccggta taaacacctt cggaactaat actgagaggg
     7741 ggaaagacag cttcaaaagt tgcttccggc tgtaaaacag gattaaaata ggaagccaca
     7801 ctttctacaa gaacattctg gctgttatat accttgaaac agacctccag ctggtcttcc
     7861 cacaatattt gaccattatt ctttactacc accacgggag aaaaagcaga gtttacatat
     7921 tcattgtttt gaggagataa aattgtttgc aatgccatat ctacagttgg aatcggatgc
     7981 acaattatct ggatagtatc cgtagctgtt aaaccggtta tattatccgt tagggtaaaa
     8041 gtgatatttt ccgttccgtt ccagttatca ctgctaaaag ttagagctaa acctgtggca
     8101 gaaatactta tatgctcagg agtttgccag gtaagctgaa tattgtttat atccgtagct
     8161 acaatatagg gagcaaaatc tacggttatg gtttctccct gccagaaagt aatactctca
     8221 ggcaggttca attcgggagt tacaattacg ggctgaccta ttttcacatc atccacaata
     8281 attccataag taacacactg ccatttaaat ccgatgtaaa tattctgtcc cgcataagcg
     8341 gaaagattat aacttgcata ttgaaaagca gttcccaagc cctgtaaatt caatatttga
     8401 gtgttgaaat tgctgatggc attgccggtt gttgaaacat aaacctgcaa gttctcagtg
     8461 tcaaaccagg cacgggtgta gaatttcagc gaatctccat tggcaatatg caattgggga
     8521 gtaatcagcc aatcgcaatt ttgattggga aaatagttat ccgcaaaggc aaaacgcgtt
     8581 ccagaatgtg cgtgactggc attttccaaa ttacgccaag tcattccatc gccgtcatca
     8641 taaactgtcc aaccagcagg aagggttgta ataccctcaa aatcctcatc tatatgccat
     8701 tgcccaaaag caaaagcaga acagcaaatg aggagacaca gtaaaaatga ttttttcatt
     8761 atagacctct tttcttaaag aaagttaagc tggggatttt caatctggat gaaccattca
     8821 atatttccgg caatatctcc accaatatct ttattgcttc cccagtaaat acttataaat
     8881 ccgctgcttt tatgtttcaa ggcattatct atattgccgc cacggatgtt atcattggtt
     8941 cccaaacaga tgagagcaac ctttctgcca tcttttccac cggtttcaat ggctttggct
     9001 aattgctgct gattgctttc tgcagaaaaa ccggttattt cattattgac gatttccact
     9061 accggatgca aaatttcttc gcccaaagca taagcacggt caccaattat ttttccctgc
     9121 attgaacccg gcttggggat aattaaaatt tcaccaccag gtaggttaat aataggggac
     9181 tgaaattcac ctttgccaaa agaaccgcat tctgcccaaa tattattgat ggcaaaacgg
     9241 aaagcggcag atttgttttc aatttccaca taattcttgt taacgaactt tctgcataaa
     9301 gccagggttt tatcgctaag catctgataa tcaatatcta aagcgcgcca gaactgaact
     9361 tcactttcaa ttgcctgttc ggaaagagtg tcatttaaaa gagaaggcat cattgccaaa
     9421 cggatattct tttcctttat aatcttacga cctttttcca gcattcggtt acggatttga
     9481 atttttttcg gatcggtaac ttccacaatt cgggattcac cattaaggtt caaataaaca
     9541 tccgcttctt tcagtttatg gttaaaattt tcataatccg cctgaaaagc aattaacgaa
     9601 tactgctccg cccttgcttg caattgttct aagtcctgat aaatcaaagt agtataaatt
     9661 cctcttttcc ctgcctccag taataatttc tccactaaag gcaaattaaa cttgcctgtg
     9721 gtaatcagca aatgcattcc cttcgtcagt ttcagcgctt catcaagcag tttatcagca
     9781 atattattca aatttatccg atattcctgc tctaaaatgc tgttaataat attgcggtta
     9841 tccaatattt tttcactggt ttccagtcca attgccaaat ccaaaacata actgcgagca
     9901 gtagctctat aatagacctc tttgttattg atatttgcct cgtcttcaat ttttagtaaa
     9961 ccttgagaaa gcatttgcgt taaattatag tgcatctttt gtttactgca accaaacaga
    10021 gttgccagtt gctgacaagt tgccggggct ttaatcaatt ctttcaagat attgtatttc
    10081 agcggactac ccatttcttt cagctgggct tgattcctga ttagttgaag attttgaatc
    10141 attttattca tccttcctta catttaattt tacataaatt acactctcct gttaccctaa
    10201 tatttagtca agaacttttt gactat
//
LOCUS       DAOOZV010000063        10086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6439_length_10086_cov_55.231283, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000063 DAOOZV010000000
VERSION     DAOOZV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6439_length_10086_cov_55.231283"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..863
                     /gene="efp"
                     /locus_tag="PLW08_06575"
     CDS             300..863
                     /gene="efp"
                     /locus_tag="PLW08_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPL52300.1"
                     /translation="MATMADIRNGMVIEFKDDLYEVIEFLHVKPGKGPAFLRSKLKNI
                     RTGRVLENTFRESDTFNEVRIERTKKEYLYRDGDFLVLMDSDNYEQMHIEASLLGDKE
                     NLLTENMEVIVSGTADGKVVGIELPTTVVQTIAECEPNLKGNTASGSGKVAYTETGLR
                     LMVPFFVEPGDKIKIDTRTGEYIERAN"
     gene            876..1082
                     /locus_tag="PLW08_06580"
     CDS             876..1082
                     /locus_tag="PLW08_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52301.1"
                     /translation="MGKAKSFAAKVAHDNSTEGKVICPVCNTEIKRIKLITNKKKNGG
                     WTPQKEYAKICKCNEADILAGKNL"
     gene            1364..4501
                     /locus_tag="PLW08_06585"
     CDS             1364..4501
                     /locus_tag="PLW08_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52302.1"
                     /translation="MPCHIWKRITLLITIVFVGSLSALVPPVPMYKNPPIAWQATRLE
                     GSFFAEGTLKTGKRNLPNNILVLRVQFSDVSFRDQAGYPDNLVHDDVFFNRWMIHLKD
                     FFLEASHLQYELDYYIYPQVLTMPHSLSFYGADGDNDIDIDLPQILPDILALIDEEVD
                     FSQYGGIIIFHSGTGQESDINSIRPNEIWSTFLTRKKLQKYFDPENDLYAGYPTDDGI
                     YLTNIIIVPEDEFQDYFPSSSEENAANYLFSMYGVLAHQLGHLLGLPTLYDNDDSNGI
                     SQGIGNWGLMGTGLWNGSGYVPAQLCAWSRYFLGWETPLVLFQNSDNNSVDYFLNHSP
                     EAIRLYKVPISTTEYFLIENRQQNPDGSLDPLSNLPSYSFKLLPAGEQDYYEDNPLTP
                     EDESLLPYFNFMENTYLGSEWDFFLPGLGGPIPDNSPFPVDGSGILIWHIDENVINAN
                     FTANFNRNYVNSNALHKGVDLEEADGIQHLDTAAYNIYKWGSPYDSFRAGNNNYFGNQ
                     YHNGMLSLPTSESYYGGIPLEITDISNSGNQMTFSVNFGWRLTTSYQGINPINACAID
                     FDGDGKDELFFPLPNGKIYIWKNEELLSNYPIQLPFYIPYNYTWDGESIYLPLQRDEV
                     CCLYKLNSAEHYYAFRSNAYSWLSHPVDIGENLALPLKINADNNAFAIKLYNKTDMTV
                     ASLADYSGKFIANLIHYDNQLSVVYQNAEGEYWLSEIDLSNYDKKEIKLPIPADSIIV
                     AVFKAPIADTENLIIQCPNSVYCLQGANIVDGFPYVHNLVAASDSSFIAPLTFADVDG
                     NGSLDILIGGESDFAIIDYSGKLMNLENQNPEPHTDFISAGIYAMDIDNDNNAEIIGN
                     FKYNRLNIWEHNYRPKIGYPVSFAERSRTLPFVSQASDNNWYIYCATDNGLLFRNKLE
                     NKPQITPACNWICEYGNLRRSASYTKMELPNQYQTDELFVPGQVYIYPNPLKSIYRQK
                     IQLNVMTSRDAELELSIFDISGTLVYRQKGYAKAYLKNLDIFDIPADKLCSGVYIAII
                     KSSTSSKRLKFAIEK"
     gene            4616..5518
                     /locus_tag="PLW08_06590"
     CDS             4616..5518
                     /locus_tag="PLW08_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPL52303.1"
                     /translation="MKHTIITLCIMLIGGMCFAGIDENAGTYGYKFLNVPYGPVSLSL
                     AGRGVFSIDNPGSFLLQPAASCMNDQHLLGITHNMWLADTQANMIAYSFAQRKSHFGI
                     AMRNLDYGEIENRDDMGFLIGNYHPLDIDVTANYAHRVTPSIYAGANLGILYQKLNTA
                     SSLALHTDLGLCWLPPVKDAKITLVGRNLGIANHTDEERVKLPVCFELDINKGFSVAE
                     QHLILGGSVIQTLDEDLKGNVNLECELFQLLTLRGGYLLGYDAQDFSAGFGVKYKNIS
                     VDYGYGAFNSELNDVHSFGVTYRF"
     gene            5531..9742
                     /locus_tag="PLW08_06595"
     CDS             5531..9742
                     /locus_tag="PLW08_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPL52304.1"
                     /translation="MKDFWTIFKLCKFWFLLLFLSLGIFVFAEITPGQLIFKTTIPVE
                     IKGTQTGLSAFDNFLSQKGVTQIKPIKGMQQPTYYLANVSEVPPAEVLEQLSFPGIQY
                     VQPNYLRKLHSTPNDPLYSQQYHYISSIPAAWNYTVGSNYVKIGIVDSGMLIHHPDLW
                     QNLAINTKDIPDNGIDDDHNGYIDDWCGWDFTDAPEMAGNALGDFLDQDNDVEDENFH
                     GTHVSGIAGAVGNNGIGVVGVCWNVSILPVRAGFRTLDNQGFLQDDDVSAAIIYATDN
                     GCNVINMSWGDPNYSAIIADACEYAYNKGVVLVASAGNDAGPGLSYPAKLSTVISVGS
                     INIAKQLSTFSSYGDDLDLVALGERVLSTYKLTTNEQYFYMDGTSMSAAFITGAVALL
                     LSLHPGLSPAEVKARLISATDDLSPVGFDIRTGHGLLNVKKLLDNLDPPFLEITSPQD
                     QLGISGSVPIIGSVYGSDFANYTVAYKKEKDQNDTNWKDVAEHIETLTKYSQPVHNDT
                     LAVFYIPNNFPEGKYLLRLQYEKTLHNMNRYNIYRTIIVDRTPPVLRTETISYFTRWT
                     KLNLEYYIAANFDEMVNAQLRITASDGLIYNVYSSLADSIQIWKLPSSIPEGMIDVQF
                     IATNIAGLTFVSDIFTNFLNIHYYLIPQYGYTKEIIGPPQKPLLRKRDYNGNGIAEYI
                     AMEIQASGYGSVKAFEPHPGGHIVTNDFGDSFWPVDIGSTYTTGSKSELLLLRGENAE
                     MWLTPAGGNYPANDDGLVVALDTGIAGGVMADYDNDGKTEILLVKNLPAERVIQAYKR
                     VSSGALAPRNTISNPTSTYSRNNFVPTIIVDNFDGDNYPDILCADTDGDVMIFEIINA
                     TQHQLCWTTRLPIGNTYSLTAGDFNGDGHKDFFVAGYVTDIVNQNLNFWLGEGFRNIG
                     NNNYTSMGSIMFNEVSSMNSITSDDLDQDGCDEVILAITPNLYVLKYINGKFVPIFYD
                     VSSNNYQVSTWEDENGIIRILGNVKTVGDNPALAEWTPEEPFTGPPTPANLTVKPLNE
                     SSIKVSWTSTGAPEYALYRKDEEGNVTRNILTGVTSFTDTNLQQGKVYKYAIASCDPS
                     YTPSESNRGPWIEGIPLPKPELVQITFAGQKELRLFFNQEMPGAIINPACYYVSNGMG
                     NPISANSISANSGVQLRFRNNFPAIDSLFVLELRNIYGISGVEPETPFYYFAYIEDII
                     PPEITGVKVLPSKQEITIQFSEEISPSSALYPANYNLHCPKNDPDNKIVSVSHSVDTV
                     TIKFAEKLKFSNYAYYLEVNNITDLAGNLISPQGNLVRFSIQNLDNLENLNVYPNPVT
                     SKHSPQVTFINFPVNKKGKIAIYSSSGQLVFQDDIGPFCAENNNITYTWNLRNNNQRP
                     VSSGIYFYVVEMGGKIKRGKLAIIK"
     gene            9782..>10086
                     /locus_tag="PLW08_06600"
     CDS             9782..>10086
                     /locus_tag="PLW08_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPL52305.1"
                     /translation="MFWKVIVAIFCSPVFTLILCIIFLLLNILDGHSTYLVLKPDKYH
                     REKNPLARWVFQKLKIPAGIIIFKTILMSILILAIAYFTAWEPLTVNIAMLIADLLFL
                     "
BASE COUNT         3055 a         1764 c         1983 g         3284 t
ORIGIN      
        1 ctccgggctt tgatgaaatc tcaaccttct aaaccttctc aaccctctta accttctaaa
       61 ccatctaaac tttctaaacc ttctcaacct tctaaacttt ctcaaccatc taaaccttct
      121 aaactttctc aaccttctca accctctaaa ctttctcaac cctcttaacc atcctatttt
      181 ttcattctaa cttcaatttt ctctttttct cttttctctt tgtgaaagaa aaaattccct
      241 tgccaaaatc cccctctcca aaagcttgtc atcataagaa taagggaaag gaaaccaata
      301 tggctacgat ggctgatatt agaaacggaa tggtcattga attcaaagat gacctatacg
      361 aggtgataga atttctccat gtaaaaccag gtaaaggacc tgcttttctg cgttcaaagc
      421 tgaaaaatat acgcacggga agggttttgg aaaatacttt tcgggaaagt gacaccttca
      481 atgaagttcg tattgaacgc actaaaaagg aatatttata tagagatgga gattttttgg
      541 tgctgatgga tagcgataac tatgaacaga tgcacataga ggcatcgtta ttgggagata
      601 aagaaaatct gcttacggaa aatatggagg ttatcgtatc gggaactgct gatggaaaag
      661 ttgtgggaat agaactgcca acaactgtgg ttcaaacaat agctgaatgc gagcccaatt
      721 tgaaaggaaa cactgcttcc ggaagtggta aagttgccta tacggaaaca ggtttacgct
      781 taatggttcc tttctttgta gaaccgggag ataaaattaa aattgacacc cgcacgggtg
      841 aatatataga aagagcaaat taaaagagga taaaaatggg aaaagcaaaa agttttgccg
      901 ctaaagtagc gcatgataac tccacagaag ggaaagtaat ctgccctgta tgcaatacgg
      961 aaataaaaag gattaagctt attactaata aaaagaagaa tggcggctgg actcctcaga
     1021 aggaatatgc caaaatttgc aagtgcaatg aagctgatat attagctggc aaaaatcttt
     1081 aaagatatac agttatctta agagaaagca atattcactt gccaaagaag acgatatact
     1141 ttcgccgttt tttacttgac tatttatgta agcaccataa aaggtctata aagctatcca
     1201 cttgcattag ttcggtagcg gattttttag ctacacaata ttatataatt tttatattgg
     1261 aaatggcagt tattttgtca gaacttgtct ggatttctgc ttttgcagga acgacaaggg
     1321 aattttcatt ttaacgataa tattattttg ggggaggatt aaaatgcctt gccatatctg
     1381 gaagcggata acattgctga taaccattgt ttttgttggt tcattatcag ctttggttcc
     1441 tcctgttcca atgtataaaa atccacctat tgcctggcaa gccacgcgtt tggaaggatc
     1501 attttttgcg gaaggaacat taaaaaccgg caaaagaaat ctacctaata acattctggt
     1561 tttaagggtg cagttcagtg atgtatcttt tcgggatcaa gcaggatatc ctgataattt
     1621 ggttcacgac gatgtttttt ttaatcgttg gatgattcat ttgaaggatt tttttctgga
     1681 agcaagtcat ttacaatatg aactggacta ttatatatat ccacaagtgt tgacaatgcc
     1741 tcattctctt tccttttacg gagctgatgg agataatgat atagatatag atttacctca
     1801 aattttacct gatattttgg ctttaataga tgaggaagtt gattttagtc aatatggagg
     1861 catcattatt tttcattccg gaaccggaca ggaatccgat attaattcta tccgccccaa
     1921 cgaaatctgg agcacttttt taacacggaa gaaactgcaa aagtattttg atccagaaaa
     1981 tgatttgtat gccggttatc cgactgatga cggaatttat ttaactaaca ttattatagt
     2041 gcctgaagat gagtttcagg attactttcc ttcctcaagt gaagaaaatg ctgctaatta
     2101 tctctttagt atgtatggag tgctggctca tcaattgggt catttattag gtttacctac
     2161 tttatatgat aatgatgata gtaatggtat cagccaggga attggcaact ggggtttaat
     2221 gggaactggt ttatggaatg gaagtggtta tgttccagct cagctttgtg cctggtcccg
     2281 ttatttttta ggttgggaaa caccccttgt gctctttcag aatagcgata ataactctgt
     2341 ggactatttt ctcaatcatt ctccggaagc aatccgtctt tacaaagtgc caatttctac
     2401 tacggaatat tttttaattg aaaaccgaca acaaaatccc gatggtagtt tagaccctct
     2461 tagtaacctt cccagttaca gtttcaaact tcttcctgca ggtgagcagg attattatga
     2521 agataatcct ttaactcctg aagacgaatc cctattaccc tatttcaact ttatggaaaa
     2581 tacctattta ggaagtgagt gggatttttt tcttcccgga ttgggaggtc cgattccgga
     2641 taacagtcct tttccagtag atgggtcggg aattcttatc tggcatatag atgaaaatgt
     2701 aattaatgcg aacttcacgg ctaattttaa tcggaattat gtaaattcca atgcgctaca
     2761 taaaggtgtt gacttagaag aagcagatgg aattcagcat ctggatactg ctgcttataa
     2821 tatttataaa tggggaagcc cttatgacag ttttcgagcc ggtaataata attatttcgg
     2881 taatcagtat cataacggaa tgctttcctt gcctacttcg gaaagctatt atggagggat
     2941 tcctttagaa ataaccgata tttctaacag cggaaatcaa atgacttttt ccgtgaattt
     3001 tggctggcga ttaactactt cttatcaggg aatcaatcct atcaatgctt gtgctattga
     3061 ttttgatggt gatggaaaag atgaattatt ttttcctctg ccgaatggca aaatatatat
     3121 ctggaaaaat gaagaattgc taagtaacta tcctatccaa ttgccttttt atatacctta
     3181 caattacact tgggatggtg aaagcattta tctgccactg cagagagatg aagtatgctg
     3241 tttatataaa ttaaatagtg cagaacatta ttatgctttc aggtctaatg cttatagctg
     3301 gttaagtcat cctgtggata taggagaaaa tctggctctg cccctaaaaa taaatgccga
     3361 caataatgcc tttgccatta aactttataa caaaaccgat atgacggtag cttctttggc
     3421 ggattatagt ggtaaattta ttgccaatct gatccattat gataatcagc tttccgttgt
     3481 gtatcaaaat gctgaaggtg aatattggct gagcgagatt gatttaagca actacgataa
     3541 aaaggaaata aaattgccta ttcctgctga ttctattatt gtagcggttt ttaaggcacc
     3601 aatagcggat acggagaatt tgattattca atgccctaat agtgtttatt gtttgcaagg
     3661 cgcaaatatt gtagatggtt ttccttatgt gcacaatctt gttgctgcct cggatagcag
     3721 ttttattgct cctctaactt ttgcggatgt tgatggcaat ggttctttgg atatcttgat
     3781 cggaggcgaa agcgattttg ccattattga ctattccgga aaattgatga atctggaaaa
     3841 ccaaaatccg gaaccgcata cggattttat cagtgcagga atatacgcta tggacataga
     3901 caatgataac aatgcagaaa taatagggaa ttttaagtat aaccggctta atatttggga
     3961 gcataattac cgtcccaaaa taggttatcc cgtttccttt gctgaacgca gcagaacctt
     4021 accttttgtt tcccaagctt ctgataataa ttggtatatt tattgtgcta ccgataatgg
     4081 cttgctgttt agaaataaac tggaaaataa gccccagata actcctgctt gcaattggat
     4141 ttgcgaatac ggaaatttaa gacgcagtgc ttcttatact aaaatggaat tgcctaatca
     4201 atatcagacc gatgaacttt ttgtgccggg acaggtatat atctacccca atcccttaaa
     4261 atccatttac cgacaaaaaa tacagcttaa tgttatgacc agtcgcgatg cagaattgga
     4321 gctaagcatt tttgatatta gtggaacttt ggtttatcgt caaaaaggtt atgctaaggc
     4381 atatcttaaa aacctggata tttttgatat tcctgcagat aaactttgca gtggggttta
     4441 cattgctatt attaaatcct caacaagcag caagcgactc aagtttgcta ttgaaaaata
     4501 atatagtttc cctacataaa tgcgaatatt ttgtcagaac ttgtttggtt taccgcttgc
     4561 tcaggaatga caaaggaatt ttcattcaaa atatgatata agagagagta aaattatgaa
     4621 acatacaata attaccttat gcataatgct gattggggga atgtgttttg ctggcattga
     4681 tgaaaatgcc ggaacttacg gctataaatt tttgaatgtt ccctacggac ctgtatcttt
     4741 gtccttagca ggaagaggtg tgttcagtat tgataatccg ggttcctttt tgttgcaacc
     4801 agcagcttct tgtatgaatg atcaacatct tttaggcatt acacataata tgtggctggc
     4861 agatacccag gcaaatatga ttgcttattc ttttgctcaa cgaaaaagcc atttcgggat
     4921 agctatgcgt aatttggatt atggggaaat tgaaaacagg gacgatatgg gttttttaat
     4981 cggtaattat catcctttgg atattgatgt tacggcaaat tatgctcatc gtgtaacgcc
     5041 ctctatttat gcaggagcta atttaggaat tttataccaa aaactgaaca ctgccagcag
     5101 tttagctttg catacagacc ttggtttgtg ttggcttcct cccgttaaag atgctaaaat
     5161 aactttggta ggaagaaatt tgggaattgc taatcatacg gatgaagaaa gagtgaaatt
     5221 gccggtttgt tttgaattgg acataaacaa aggattttct gttgcagaac agcatttaat
     5281 cttaggcggt tcggtaattc agaccttaga tgaagacctt aaaggtaatg ttaatttaga
     5341 atgtgaactc tttcaattac ttactctaag aggtggttat cttttgggct atgatgctca
     5401 ggattttagt gccggctttg gagttaaata taaaaatatc tctgtggatt atggctacgg
     5461 tgctttcaat agtgaattga acgatgttca ctcttttggt gtaacttaca gattttagat
     5521 agaggaaaaa gtgaaggact tttggaccat atttaaactg tgcaaattct ggtttcttct
     5581 cctgttttta agcttgggta tttttgtttt tgccgaaatt actcctggtc agcttatttt
     5641 taaaaccact attcccgttg aaattaaggg aacccaaacc ggattatctg cttttgacaa
     5701 ttttcttagc cagaaaggag ttacgcagat aaagcccatt aagggtatgc aacaacccac
     5761 atattatctg gcaaatgttt ctgaggtgcc tcctgctgag gtcttggaac aattaagttt
     5821 tccgggtatt caatatgttc agcccaatta tttgagaaaa ttgcactcaa ctcccaacga
     5881 tccattatac agtcaacagt atcattatat cagcagcatt cccgctgcct ggaattatac
     5941 ggttggcagt aattatgtta aaatcggtat tgtggattca ggaatgttaa ttcaccatcc
     6001 cgacctgtgg caaaaccttg ctatcaatac caaggatatt cccgataacg gaattgatga
     6061 tgatcataac ggctacattg atgattggtg tggatgggat tttactgatg cacccgaaat
     6121 ggcaggtaat gctttagggg acttcttaga tcaggataat gatgtggaag atgaaaattt
     6181 tcacggaact catgtttcgg gcattgcagg cgcagttgga aataacggaa taggagttgt
     6241 tggggtctgt tggaatgtaa gtattttacc tgttagagct ggttttagaa cacttgataa
     6301 tcagggcttt ttacaagatg atgatgtttc cgctgctatt atttatgcca cagataacgg
     6361 gtgtaatgtg attaatatga gttggggtga tcccaattat tctgctatca tagctgacgc
     6421 ctgtgaatat gcttataaca aaggagttgt attagttgcc tcggcaggaa atgatgcagg
     6481 acccggttta agctatcctg ccaagctttc tacagttatt tctgtcggtt caatcaatat
     6541 tgccaagcag ctttccactt tttccagtta tggagatgat ttagaccttg tagcattagg
     6601 agaaagagtt ctatctactt ataaacttac tactaatgaa caatatttct atatggatgg
     6661 aacctctatg agtgcagctt ttataacggg cgctgtggca ttactcctat ctttgcatcc
     6721 tggtttaagt cctgcagaag ttaaagctcg tttaatttct gctacggatg atttatctcc
     6781 tgtcggcttt gatataagaa ccggtcatgg tttgttgaat gttaagaaac tgctggataa
     6841 tttagaccct ccttttttag aaattaccag tccgcaggat caattaggaa ttagtggttc
     6901 ggttcctatt ataggttctg tctatggttc tgattttgcg aattatactg ttgcctataa
     6961 gaaagaaaaa gaccaaaatg acactaactg gaaagatgtt gcagaacata ttgaaacgct
     7021 aacaaaatat agtcagccag ttcataacga tactctggct gtattttata ttccgaataa
     7081 tttccccgaa ggaaaatatc ttctgcgact gcaatacgaa aaaaccttgc acaatatgaa
     7141 ccgctataat atttatagaa caattattgt agatcgcact cctccagttt taagaacgga
     7201 aactatctcc tattttaccc gttggacaaa gctaaatctg gaatattata ttgcagctaa
     7261 ttttgatgaa atggttaatg ctcagctcag aataaccgct tcagatgggc taatctataa
     7321 tgtttattct tctcttgcgg atagcattca gatttggaaa ttaccttcct ctattccaga
     7381 aggaatgatt gatgtccaat ttattgctac caatattgct ggtttaacct ttgtttctga
     7441 tatctttact aattttttga acattcatta ttatctaatt cctcaatatg ggtatactaa
     7501 agaaattata ggacctcccc aaaagccgtt gttgcggaaa agggattata acggcaatgg
     7561 catagctgaa tatattgcta tggaaattca ggcaagtggt tatggttctg taaaagcatt
     7621 tgaaccccac cccggaggac atattgtaac aaatgatttt ggggattctt tttggccagt
     7681 tgatatcggt tccacttata ctacaggttc taaaagtgag ttgcttttgt tgagaggcga
     7741 aaatgctgaa atgtggctta cccctgcagg cggaaattat cccgctaatg atgacggatt
     7801 agtagttgct ttggatacag gaattgcggg aggagtgatg gcggattatg ataatgatgg
     7861 caaaaccgaa atcctcttgg ttaaaaattt acctgccgaa cgggttattc aagcatataa
     7921 aagagtttcc tcaggtgctt tagcgccaag aaatactatt agcaacccta cttctactta
     7981 tagccgtaat aactttgttc ctacaataat tgtagataac tttgatggtg ataactatcc
     8041 cgatatttta tgtgccgaca ctgatggtga tgtaatgatt tttgaaatta ttaatgcgac
     8101 acaacatcaa ttatgctgga caacccgact tcctatagga aatacatatt ctcttaccgc
     8161 cggtgatttt aatggcgatg gacacaagga tttctttgtt gctggttatg ttaccgatat
     8221 tgttaatcaa aatctcaatt tctggttagg agagggattt cggaatatag gtaataacaa
     8281 ttatacctct atgggcagca ttatgttcaa tgaggtctca tctatgaatt ccattaccag
     8341 tgacgacttg gatcaggatg gctgtgatga agttatttta gctattaccc ccaatttata
     8401 tgttttgaaa tatattaatg gcaaattcgt tcctattttc tatgatgtaa gctctaataa
     8461 ttatcaggtt agcacttggg aagatgaaaa cgggattatt cgcattttgg gtaatgtaaa
     8521 aacggtagga gataatcccg ctttggcaga atggactcct gaagaaccgt ttacaggacc
     8581 tcccacacct gccaatttaa ctgtgaaacc cttaaatgaa agctctataa aggtttcctg
     8641 gacctctacc ggtgcaccgg aatatgctct atatagaaaa gatgaggaag gaaatgtaac
     8701 caggaatatt ttaactggtg ttactagttt taccgatact aatttgcaac agggcaaagt
     8761 atataagtat gccattgctt cctgtgaccc ttcttataca ccaagtgaaa gtaatagagg
     8821 accctggatt gaaggaattc ctttgcctaa acctgaattg gtgcaaatta cttttgcggg
     8881 acaaaaagaa ttacgtttgt tcttcaatca agaaatgccg ggagctatta tcaatcctgc
     8941 ctgttattat gtgagcaacg gaatgggcaa tcctatttcc gctaatagta tttccgctaa
     9001 ttctggtgtc caattgcgat ttaggaataa ttttcctgct attgattccc tttttgtttt
     9061 agaacttaga aatatatatg gaatcagtgg agtggaacca gaaacccctt tctattattt
     9121 tgcctacata gaagatataa ttcctcccga aattaccgga gttaaggttt tgccttccaa
     9181 acaggaaatt actatccagt tcagtgaaga aatatcacct tcgtcggctt tgtatcctgc
     9241 taattataat ttacattgcc ctaaaaacga tcctgacaat aaaattgttt ctgtttctca
     9301 ttctgtggat acggtaacaa ttaagtttgc agaaaagctg aaatttagca attatgctta
     9361 ttatttggaa gtgaataaca taaccgatct ggcaggaaac ttgatttcac ctcaaggtaa
     9421 tttagttcgc tttagcattc aaaacctgga taatctggaa aacttgaatg tttatcccaa
     9481 tcctgttact tccaaacatt caccgcaagt tacatttatc aactttccgg taaataaaaa
     9541 agggaaaatt gccatctaca gcagtagcgg acaacttgtt tttcaagatg atataggacc
     9601 cttttgtgca gaaaacaata atattactta cacctggaat ttacgaaata acaatcagcg
     9661 tcctgtttcc agtggaattt atttctatgt agtggaaatg ggaggcaaaa tcaaacgcgg
     9721 taaacttgcc atcattaaat aaatattgta gtaaataatg cttacacaga attaggatta
     9781 tatgttttgg aaggtaattg ttgccatatt ttgttctcca gtgtttaccc ttattttgtg
     9841 cataattttc cttctgctga acattctgga tgggcattct acttatttgg tgctgaaacc
     9901 ggataaatat caccgagaaa aaaatcccct tgcccgctgg gttttccaaa aactgaaaat
     9961 ccctgccggt ataattattt tcaagaccat tttgatgagt attctgattt tggctattgc
    10021 ttattttact gcctgggaac cgttaaccgt taatattgca atgcttattg cagaccttct
    10081 ttttct
//
LOCUS       DAOOZV010000064        10037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6462_length_10037_cov_59.874574, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000064 DAOOZV010000000
VERSION     DAOOZV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_10037_cov_59.874574"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..3406)
                     /gene="mfd"
                     /locus_tag="PLW08_06605"
     CDS             complement(23..3406)
                     /gene="mfd"
                     /locus_tag="PLW08_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPL52306.1"
                     /translation="MLYGKIEEILAQSKFMQRVLKLPLKDRSIQIYHLNQSARALLAV
                     HLWKHTGKNIILVSQDDIIAEDIWDDLCVLIGKENAHYLPDYEILPYEERSPHYSIRA
                     TRMETFLATLNNNKSAIYSLSARALGRYLPAKKFLSRHILHLKQGMECEPDTLLHNLY
                     DMGYEIQYQVSKVFQAAKRGGIIDIFSPPLTNPVRLEFWGDEIIGMRVFSVTTQRSLE
                     QYLTELTILPARELSLSDVDSGSPIIAKIRNQGFFEGIENYYALLCNELQTFADYFEK
                     DNRILVWNNFYYLQEEYETLFEQALTTWQKEAKIQGQGRVPRPEQMFADWYVLTELKE
                     KSEKIYLSQSEFELSFPTLNMRAPFASQPNFESDLGLLAETLFSRNKEGWQNILLFDN
                     QSQAKRMQQSIGKVPFIEFFGVLHEGFSLEDCHLNVWTDHEIFNRYKSRRYTPRYAPG
                     ETIVNYEDLKPGDYVVHIDHGIGVFEGLKIIRLDGSDVECLVLRYANDDRVYVPTYQL
                     SLVTKYVAEESAKPVLNKLGSSKWNNTKRKAAQQIELIAADIVKLYAERSARLGIAHQ
                     PDTEWQKELEDSFIYEDTPDQSKATKEIKEDMELPAPMERLLCGDVGFGKTEVAIRAA
                     FKAVCSGYQVAVLAPTTLLVEQHYRVFRERLAQYPVKIVMFSRFRKNTAMKKDLLGLK
                     SGSIDIAIGTHRLLSKDVQFKKLGLLIIDEEHRFGVRHKEKLRALQSNVDTLYMSATP
                     IPRTLNMALSKLKEISLMQTSPKERLPVRTIITPRDMEVIKDAIRREIDRGGQVFFIH
                     NRVQTIETVATELRNAMPKVRFIVGHAQMPEQQLERVMADFLAKEYQVLISTTIIENG
                     IDIPNANTILIDNAETFGLAQLYQMRGRVGRSNRRAYAYLLISKGTTTVARKRLEALT
                     QYDYLGAGFQVALRDLELRGAGTILGTKQSGIIQAIGFNYYNRILANAIQAVEKGETT
                     KLFNDESPDTRRKVRTEIDLYFPPDYIDDDEERLRIYKRLSELETLEDINNFEVELLD
                     RFGPLPEQASWLLNYFQLSQLAKKLSLTDCHVKHDSFIMEFDAKKLPSKEQLLHFTTK
                     IKQPTRFEAGKNLKVIIDLDPEASYLQQFETAIEILKIW"
     assembly_gap    3429..3528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3540..5717)
                     /locus_tag="PLW08_06610"
     CDS             complement(3540..5717)
                     /locus_tag="PLW08_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPL52307.1"
                     /translation="MDHNFYASQLNERQLEVVTDTENPVLVLAGAGSGKTRSLIYRTA
                     YLIKEKQVHPWNILIVTFTNKAARELQERLEKLLNIPVRSLWVGTFHAICCRILRFES
                     AHLPFNANFSIYDEDTQKSLLKKIYKEYGFDKQSYPINNILTRISRYKNKLMLPEDIK
                     FQGEDKDSEFATFLNIYRLYQQQLLLNQALDFDDILLWTAKLLLNNNTVREKYRAQFQ
                     YVMIDEYQDTNKAQFEIVHQIAGGHQRVCVVGDDDQAIYSFRGASLRNILEFEKDYNS
                     VHSIRLEQNYRSTGNILALANAVIANNRRRHSKELWSELPEGDKPVLAVYENEIEEAN
                     RITDAILKLQKKGIPLREIAVLYRTNAQSRVFESALIQAEIPYSIVGGVQFYQRKEIK
                     DLLAYLYILSNLNDNESLLRIINEPARGIGQTSINRLISFANKTRISLFRAIQNVEAI
                     EELGNPAKKRISEFCLLLERWQQSVSRKPALEMVKTIVEELGLISHYQNSNDPQDIAR
                     AENLVEFVTSVSEFSEHFYQEYEREPLIADYLPYVALQTDLDRVKENMDSIRLMTLHN
                     AKGLEFEAVFIAGLEDELLPHRMCMNNQDSIEEERRLFYVGITRAKNYLHLSYTKSRR
                     LYDTFYFPVPSQFLAEVDSRLFYHSDGDFGIAQPPKRIKKTNKVITESQKYYKIGQKV
                     FHPEYGLGVILSVDGEGEDARLTISFGNRLAKIIGRYLMKNEE"
     gene            complement(5720..6145)
                     /locus_tag="PLW08_06615"
     CDS             complement(5720..6145)
                     /locus_tag="PLW08_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52308.1"
                     /translation="MVKHLAKIIILIMLIILLASCSLKRSNPLDPNGNGDIVVPEPVT
                     GITHNTSSQHQNPCWVNIKWAANSASNTDGYFIYRGIGYYSSFSLVGDVKTNEFMHSS
                     ADDPTVRPGDYYYRVSAYKTYPEGKLEGRRSEPYWVRIP"
     gene            complement(6146..6748)
                     /locus_tag="PLW08_06620"
     CDS             complement(6146..6748)
                     /locus_tag="PLW08_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52309.1"
                     /translation="MKARTKIFLFLLLCIICGTTALPLKAQSFDIKAFSDTTKYGWKN
                     YLDRNAYRQDLKQRQDLLQIYEMEAQPLNTNILKSAIIPGWGQFSTKESTKGTVILGT
                     EIVLAGTSFYFLDRALSKYKLYKQATQVDEIEKYYKDAQVPYQYSFILMGAAGIIWAY
                     NIFDVIQSTQDYNVRLWEEIVERSKSGPVYITPTGIEVRF"
     gene            complement(6745..8151)
                     /locus_tag="PLW08_06625"
     CDS             complement(6745..8151)
                     /locus_tag="PLW08_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52310.1"
                     /translation="MFTRYRIILLLLIVIILSGCEINRLRSAEELYRKNLYAAAIVEL
                     DDLIANAENRAIATRAEIVRSNCYYALAEAAVEKKNWPLAIRFFKLANSDSANLRLAE
                     IYRDLAMEAEKEGNRIVSKSYLDAIIREVPESPLIPEVLYRRIGYFLDVKADNESAWN
                     DYMRLFDNYPNSPYEILARNYVMRFIPGKLKYAEVLTEQGYYADALKELFEISKYPVV
                     DMNQVNKLISDVYEKQAEELLSEQDYIEADRLFRIAMQYYPQKKESIIHRLESITNLF
                     IQKGNSLLEAGDFQGALVHYRKTFEIIPDYPPALEAIDRLFTIQENIRKAADLYKQGE
                     QLEATGKLNEALQAYNSAYALDPKPEYKQKANLVQNMIEANKNPAAFARKIITEYKGG
                     LLTNRIQNQKKELLKRYKEYEIQDSGWKLMLSTGQYKYEARYDLLTPNETYLYVWQIN
                     LRDRSIIPLNKLSEALMQ"
     gene            complement(8135..8872)
                     /locus_tag="PLW08_06630"
     CDS             complement(8135..8872)
                     /locus_tag="PLW08_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL52311.1"
                     /translation="MELTILGSGPGLPQLDKHLSSILIRKENNLILADCGDSCAHRLL
                     EHKVAAEELDAIFITHYHPDHLDGLFMVLQMLYLQNRKRDLLLFLPERPASFLEILQL
                     MYTYPQKFGFNLLIRDIEQAELYFDWIYAVPNDHLYRYASIISEYKLPNQMKSWSLCF
                     TGKQGKFVYTSDIETTDCIMDTLKDAHTVLVDAGHPEAEQIIKLKYTAIKKIILTHGI
                     TPELEDRKDELNPEIFEFAREDFVYQI"
     gene            complement(8875..9972)
                     /locus_tag="PLW08_06635"
     CDS             complement(8875..9972)
                     /locus_tag="PLW08_06635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPL52312.1"
                     /translation="MENRIYEYLSVFIMAWLFVYGLTPFIIRLASALNFVDKPEARKI
                     HLKNIPLLGGLSVATGFVLLCIYDVLITPGRYLDKPMLGYLAGSIIIVLIGIIDDHKG
                     MKPAVKLAGQIAVSLLFIGTNFTVGELGLMFGSIYLSLPIMLLWMVGLMNALNFLDNM
                     DGIISGMAGILGLGFFAFALVNTTQANQEAMALMALIALSFAGSTFGFLPYNFNPARI
                     FLGDAGSMFIGYFLSSMGILMARYAANRYNDMFFYLLPVLLLSYAIFDISLVSFTRRR
                     DGRQITQGGRDHSTHRMNTILGSVKITAIIIYTINVLIALTSIMIFYIGNRHLLIITT
                     VIFALFFLFIGRKLDKVPIVVPANQQKHKKE"
BASE COUNT         2955 a         2091 c         1756 g         3135 t
ORIGIN      
        1 ggtgtaaaaa tccccttgca ggttaccata ttttcaggat ttctatcgcc gtttcaaact
       61 gttgtagata gcttgcttcg gggtctaaat caataataac tttcaggttt tttcctgctt
      121 caaagcgcgt tggttgttta attttggtag taaaatggag caattgttct ttagaaggta
      181 gttttttagc gtcaaattcc ataataaagc tatcgtgttt aacatggcag tccgttaaag
      241 acaatttttt agctaattgg ctcagctgga aatagttcag aagccaactt gcctgttctg
      301 gtaaaggtcc gaagcggtct aaaagttcca cctcaaaatt atttatatct tccagcgttt
      361 ccagttcact taagcgtttg taaatacgca agcgttcttc atcgtcatca atataatctg
      421 gaggaaaata gaggtctatt tcggttctta cttttcttct tgtatcgggt gattcatcat
      481 taaataattt ggttgtttcg cctttttcca cagcttgaat ggcattagcc agaatgcgat
      541 tataatagtt aaagccaatt gcctgaataa ttccgctttg tttagttccc aaaatggttc
      601 ccgcacctct taattccaaa tctcgtaagg caacttgaaa gccggcaccc aaataatcat
      661 attgggtcaa tgcctccaag cgttttctgg caactgttgt agttcctttg gaaataagca
      721 gataggcata agctcttcgg ttacttcttc ctactctgcc gcgcatttga taaagttgtg
      781 ctaaaccgaa tgtttcggca ttatctatta aaatcgtgtt agcattaggg atatcaatac
      841 cgttttcaat gatagtagta gaaattagca cctgatattc ctttgccaaa aagtcagcca
      901 ttaccctttc cagttgttgt tccggcattt gagcatgacc tacaataaaa cgaactttag
      961 gcattgcgtt tcttaattct gttgctactg tttcaattgt ctgcacacgg ttatgaatga
     1021 aaaaaacctg tcctccccgg tcaatttctc ttctgatggc atctttgatt acttccatat
     1081 cccgtggggt aataatagtc cgaacaggta atctttcttt cggcgaggtc tgcatcaggg
     1141 aaatttcttt cagtttggaa agtgccatat tcaaagttcg cggaatggga gttgcgctca
     1201 tataaagtgt atccacattg ctttggaggg ctcttagttt ttctttatgg cgcactccga
     1261 aacgatgttc ctcatcaata attaaaagtc ccaatttctt gaattgaaca tctttggaaa
     1321 gcaaacgatg ggttccgatt gcgatatcaa tagagccact tttaagcccc aaaaggtctt
     1381 ttttcatagc ggtgtttttc ctaaaacggc taaacattac aattttaaca ggatactgag
     1441 ccaagcgttc tctaaaaacc ctatagtgct gttctaccaa taaagttgta ggtgctaaaa
     1501 cagcaacctg ataaccgctg cagacagctt taaatgcagc tcggatagca acttccgttt
     1561 tcccgaaacc aacatctcca caaagcagtc gttccatcgg tgctggaagt tccatatctt
     1621 ctttaatttc tttcgtggct ttgctttgat cgggggtatc ctcatagata aaagaatcct
     1681 ccaattcctt ttgccattcg gtatccggtt gatgagcaat accaagacgt gcacttcttt
     1741 ctgcatatag cttaacaata tcggctgcaa taagttcaat ttgttgagca gcttttcgct
     1801 tagtattatt ccatttggaa ctgccaagtt tgtttaaaac cggtttggca ctttcttccg
     1861 ctacatattt ggttaccaga gaaagttgat aagtaggaac ataaacccga tcatcattag
     1921 cataacgcaa aaccaggcat tcaacatcgg aaccgtccaa acggattatt ttcaatcctt
     1981 caaaaacacc aatgccgtga tctatatgta caacatagtc ccctggcttg aggtcttcat
     2041 aatttacaat agtttctccc ggtgcataac ggggagtata gcgtctgctt ttataacgat
     2101 taaagatttc gtgatctgtc catacattta gatggcaatc ttccagggaa aagccctcgt
     2161 gcaaaactcc gaagaactca ataaaaggaa ctttgccaat actttgctgc atacgctttg
     2221 cctgactttg attgtcaaaa agtaaaatat tctgccagcc ctctttgttt ctggaaaaaa
     2281 gggtttctgc cagtagtccc aaatcggatt caaaattggg ttgacttgca aaaggagcac
     2341 gcatattcaa agtggggaag gagagttcaa attcactttg agaaagataa attttttcac
     2401 ttttctcttt caattctgtt aaaacatacc aatcggcaaa catttgttcc gggcgcggaa
     2461 ctcttccctg accttgaatt ttggcttctt tttgccaggt ggtaagtgct tgctcaaaaa
     2521 gggtttcata ctcttcctga agatagtaaa aattgttcca aaccagaatg cgattatcct
     2581 tttcaaagta atcagcaaag gtctgtaatt cattacatag caatgcataa taattctcaa
     2641 ttccttcaaa gaaaccctga ttccgaattt tggcaattat cggtgaacca ctatcaacat
     2701 cgcttaaaga aagttccctg gcaggtaaaa ttgtcagttc cgttaagtat tgttccaaac
     2761 ttcgctgtgt agtaactgaa aaaacacgca ttccaataat ttcatcaccc cagaattcca
     2821 aacgcacagg attagttagc ggaggtgaaa aaatatctat aataccgcct ctttttgctg
     2881 cctgaaagac cttgctaacc tgatattgaa tttcataacc catatcgtat aaattgtgaa
     2941 gtaaggtgtc gggctcacat tccataccct gtttaagatg taaaatatga cgggagagaa
     3001 attttttggc aggtaaatag cgtcctaaag ctcttgccga aagcgaataa atagcacttt
     3061 tattattatt aagggtagca agaaaggtct ccattcgcgt tgcccgaata ctgtaatgcg
     3121 gagaacgttc ttcatagggc aaaatctcat aatcgggcaa ataatgtgca ttttcctttc
     3181 cgataagtac acagaggtca tcccaaatat cctctgctat gatatcatcc tgagaaacaa
     3241 ggattatatt ttttccggta tgtttccata aatgaactgc taataatgcc cgggcacttt
     3301 gattgaggtg gtaaatttga atagacctat cttttaaggg aagcttgagc accctttgca
     3361 taaatttact ttgggctaat atctcttcta ttttaccgta gagcattgtc tctcaccgat
     3421 ttcacggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc aaccctcttt
     3541 cattcttcat tcttcattaa atatcttcct attatttttg ccaatctatt tccaaaagat
     3601 attgtgagtc gtgcatcctc tccttcacca tctacgctta aaataactcc taatccgtat
     3661 tccggatgaa agaccttttg tccgatttta taatattttt ggctttcggt gatcacttta
     3721 ttcgttttct ttattctttt cggtggttga gcaatgccaa agtcaccatc cgaatgataa
     3781 aataatcggg aatccacttc tgccaaaaat tggcttggaa caggaaaata gaaggtatca
     3841 tacaggcgtc ggctttttgt ataacttaaa tgcagatagt tctttgctct ggtaattcct
     3901 acataaaaca ggcgtctttc ttcttcaatg ctatcctgat tgttcataca cattctgtgg
     3961 gggagaagtt catcttccaa accggcaata aacactgctt caaattccaa gcccttagca
     4021 ttgtgcaagg tcatcaagcg aatggaatcc atattttctt tcactctatc caaatccgtt
     4081 tgcaaagcta catagggcaa atagtcagca attaacggtt ccctttcata ttcctgataa
     4141 aaatgctcac tgaattcact aacggaagtt acaaattcca ccaggttttc cgctcgggcg
     4201 atatcctgag gatcattact attctgataa tggctaatca atcccaattc ttccacaatt
     4261 gttttcacca tttccaaagc aggtttgcgg gaaacgcttt gctgccatct ttccagcagc
     4321 aggcaaaatt cactgattct ctttttagcg ggatttccca gttcttcaat tgcctccaca
     4381 ttttgaatag cgcggaaaag agaaatccgc gttttattgg caaaactgat cagacggtta
     4441 atacttgttt gtccaattcc ccttgctggt tcattgataa tgcgtaaaag ggattcgtta
     4501 tcattcagat tacttaaaat atataaatat gccagcaggt ctttaatttc tttgcgctga
     4561 tagaattgca cgcctcccac gatgctgtaa ggaatttctg cttgaattaa agcactttca
     4621 aaaacccggg actgcgcatt ggttctatat aaaacagcta tttcgcgtaa aggaattcct
     4681 tttttctgca gtttaagaat ggcatcggta attctgtttg cttcctcaat ttcgttttca
     4741 taaacagcaa gaacgggttt atcaccctca ggaagttcac tccaaagctc tttactgtgt
     4801 cttcttcggt tattggctat aacagcattg gctaaagcta aaatattgcc tgtgcttctg
     4861 taattttgtt ccaggcgaat gctatgcacc gaattataat ccttttcaaa ttccagaata
     4921 ttgcgtaaac ttgcaccccg aaagctgtaa attgcctgat catcatctcc tacaacgcaa
     4981 accctttgat ggcctccggc aatttgatgc actatttcaa actgcgcttt attggtatcc
     5041 tgatattcat caatcatcac atattgaaat tgagcgcggt atttttctcg cactgtgtta
     5101 ttatttagca aaagtttagc tgtccacagc aaaatatcat caaaatccaa tgcctgattg
     5161 agcagaagtt gttgctgata aagccggtaa atattcaaga aagtggcaaa ttcgctatct
     5221 ttatcttccc cctgaaattt aatatcttcc ggcagcatca gtttgttttt atatctactg
     5281 atgcgagtta atatattgtt aataggatag ctctgtttat caaagccata ttccttgtaa
     5341 atcttcttca aaagggactt ttgggtatct tcatcgtaaa tggaaaaatt ggcgttaaaa
     5401 ggcagatgag cactttcaaa gcgtaaaatg cgacagcaaa ttgcgtgaaa agtgcctacc
     5461 cataatgacc ttaccggaat attcagtaat ttttccaggc gttcctgcaa ttcccgagct
     5521 gctttattag tgaaagtaac aatcaaaata ttccagggat gcacctgttt ttccttaatg
     5581 agataagcag tcctgtagat tagggagcgt gtttttccag aaccggctcc cgctaaaact
     5641 aaaaccggat tttctgtatc ggtaaccact tccaactgcc tttcgttcag ttgagaagca
     5701 taaaagttat ggtccatagc taaggaatac gaacccaata gggttctgac ctacgccctt
     5761 ccagtttgcc ctctggatat gttttataag cggaaacccg ataataataa tcaccgggac
     5821 gcacagtagg gtcatcagca ctgctatgca taaattcgtt agtttttaca tcacccacta
     5881 aagaaaaaga agaataataa ccaattcccc tgtaaataaa atatccatct gtgttggatg
     5941 cactattagc tgcccatttt atatttaccc agcagggatt ttgatgttgt gatgaagtat
     6001 tatgagtaat tcctgtaacc ggttccggaa ctacaatatc tccattgcca ttaggatcaa
     6061 gaggattact gcgtttcaaa ctgcaggagg caagtaaaat tatcagcata atgaggatta
     6121 ttatttttgc caagtgtttc accatttaaa acctcacttc tattcctgtg ggagtaatat
     6181 acacaggacc gcttttactg cgttccacaa tttcctccca taaacggaca ttataatcct
     6241 gcgtggattg aattacatca aagatattat aagcccaaat aattcctgca gcacccatca
     6301 ggataaagga atattgatag ggaacctgag catccttata atacttttct atttcatcta
     6361 cctgagttgc ctgtttatat agtttatatt tacttaaagc tctatccaga aaatagaaag
     6421 aagttcctgc cagcactatt tctgtgccta aaataacggt gccttttgtg ctttctttgg
     6481 tagaaaattg tccccaaccg ggaattatag cggatttcag gatattggta tttaaaggtt
     6541 gagcttccat ttcataaatc tgtaataaat cctgcctttg tttcaagtcc tgacgatagg
     6601 catttctatc cagataattt ttccagccgt attttgtagt atcggaaaat gctttaatat
     6661 caaagctctg tgcttttagg ggaagagctg ttgttccgca aataatgcac agcaaaagga
     6721 agaggaatat tttagttctt gctttcattg catcaatgcc tcagaaagct tgtttaaagg
     6781 tataatactt ctatctctta gatttatttg ccacacatac aaataggtct cattaggagt
     6841 aagcaaatcg tagcgtgcct catatttata ttgtcctgta gaaagcatca atttccatcc
     6901 gctatcttga atttcgtatt ccttatagcg tttcagcaat tccttctttt gattttgtat
     6961 acggttagtt aataatccac ctttgtattc agtaataatt ttacgggcaa aagcagcagg
     7021 attcttgttt gcctcaatca tattctgaac taaatttgcc ttttgtttat attccggttt
     7081 ggggtctaag gcataggcag aattatatgc ctgcaatgcc tcattcagtt tacccgttgc
     7141 ttccagttgt tcaccctgtt tataaagatc agcggctttg cggatatttt cctgaattgt
     7201 aaataaacgg tctattgcct ccagggcagg aggataatcg ggaataattt caaaggtctt
     7261 acgataatgc actaaagcac cttgaaaatc acctgcttcc agtaaactgt ttcctttttg
     7321 gataaagaga ttagtgatgg attccaggcg atgaataatg ctctcctttt tttgaggata
     7381 atattgcatt gcaattctaa acaaacggtc tgcttctata taatcctgtt cgctaagcag
     7441 ttcttctgcc tgtttttcat aaacatccga aatcagttta ttcacctggt tcatatctac
     7501 aaccggatat ttggaaattt caaatagctc ttttaaggca tcggcatagt agccctgttc
     7561 tgttaagacc tcagcatatt tcaattttcc cggaataaaa cgcattacat aatttcgtgc
     7621 cagaatttca taaggactat tgggatagtt atcaaaaaga cgcatatagt cattccaagc
     7681 cgattcatta tctgccttca catctaaaaa atatccaatt ctgcgataaa gcacttcagg
     7741 aattaaagga ctttccggaa cctctctaat tatagcatcc agatagctct tgcttactat
     7801 ccggttacct tctttttccg cttccatagc aaggtcacga taaatttctg ccagacgcaa
     7861 attggcggaa tccgaatttg ccagcttgaa aaaacgaatt gccaggggcc agttcttttt
     7921 ctctacagct gcttctgcta aggcataata acaattggaa cgcactattt cagccctggt
     7981 ggctatagct ctattttcag cattagcaat cagatcatcc aattccacaa ttgctgcagc
     8041 gtaaagattt ttccggtata actcttccgc acttcttaag cggttaattt cacagccgct
     8101 aagaattatt actataagta gcaggagaat aattctatat ctggtaaaca aaatcttctc
     8161 tggcgaattc aaatatctca ggattcagtt catctttccg gtcttccaat tccggggtta
     8221 ttccatgggt gaggattatt ttctttatgg cagtatattt cagttttatt atctgttccg
     8281 cttcaggatg acccgcatcc actaaaacgg tatgtgcatc ttttaatgta tccataatgc
     8341 aatcggtggt ctctatatca ctggtataaa caaattttcc ctgttttccg gtaaaacaaa
     8401 gtgaccagga tttcatctga ttgggcagtt tatattcact gattatagat gcataccgat
     8461 acaaatgatc attgggcaca gcataaatcc aatcaaaata tagctctgcc tgttctatat
     8521 cccgaattaa caaattaaaa ccgaatttct gagggtaggt atacatcaat tgtaagattt
     8581 ccagaaagga agcaggacgc tccggtaaaa acagcaaaag atcgcgcttt ctattttgta
     8641 ggtataacat ttgcaaaacc ataaacagac catcaaggtg atcgggatgg taatgtgtaa
     8701 taaaaatggc atccagctct tcggcagcaa ctttatgctc cagtaaacgg tgtgcacagc
     8761 tatctccaca atcagccaga attaaattat tctctttccg aataagaata ctggaaagat
     8821 gtttatcaag ttgtggcaaa cctggtcccg aacctaaaat agtcagttcc atatttactc
     8881 ctttttgtgt ttttgctggt ttgccggaac cacaatgggc accttatcca gcttgcgtcc
     8941 aataaataaa aagaaaagtg caaaaataac ggtcgttata atcagtaaat gccgattgcc
     9001 aatgtagaaa atcattattg aagttaacgc tatcagcaca ttgatggtat aaataataat
     9061 ggcagtaatt ttaactgagc ccaaaatagt attcatcctg tgcgtactat gatctcttcc
     9121 tccttgggta atttgccttc catcccttct gcgggtgaaa cttaccaaag aaatatcaaa
     9181 aatagcataa gaaagcaaaa gcaccggtaa taaatagaaa aacatatcat tataacgatt
     9241 ggcagcataa cgagccatca aaattcccat tgaggataaa aaataaccga taaacatact
     9301 gcccgcatca cctaaaaata tgcgcgccgg attaaaatta tagggcagaa aaccaaaagt
     9361 agaaccggca aaactaagag caatcaatgc catcaaagcc attgcttcct gatttgcctg
     9421 cgtcgtattg accaaagcaa aagcaaaaaa ccccagccct aaaatgcctg ccattccact
     9481 tataatacca tccatattat ccaaaaaatt cagcgcattc atcagcccta ccatccaaag
     9541 taacataatc ggtaaggaaa gatatatgct gccaaacatc aatcccaatt cgccaacggt
     9601 aaaattcgtg ccgataaaaa gtaaacttac cgctatttga cctgccaatt ttaccgctgg
     9661 tttcattccc ttgtgatcat ctattatccc tattaaaaca attattatag aaccggctaa
     9721 ataacccagc atcggtttgt ccaaataacg acccggagta attaacacat cataaatgca
     9781 aagcaataca aaaccggtgg ctacactcaa acctcctaaa agagggatgt ttttcaagtg
     9841 tattttacgc gcttcgggct tgtctacgaa gttcaaagca cttgccaaac gaatgataaa
     9901 aggcgttaaa ccatacacaa atagccacgc cattatgaat acacttaagt attcataaat
     9961 ccggttttcc atcgcaccgc tccactattt tctgaccaag caaaaaaaga aagagatttt
    10021 gtcaagaacg aatgaag
//
LOCUS       DAOOZV010000065         9382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_6946_length_9382_cov_54.791894, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000065 DAOOZV010000000
VERSION     DAOOZV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_9382_cov_54.791894"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..2991
                     /locus_tag="PLW08_06640"
     CDS             220..2991
                     /locus_tag="PLW08_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPL52313.1"
                     /translation="MKKFLIMLFVLAGLSVLTAKVDLNTASLAELMQLPITEKQAKDI
                     YEYREFVKIFDDIYELRKIPSIDQETLDRLKPLVVVSIYVETDEAAVRREEIRDLLER
                     VDSNEGASEGMADVWEDYLMTPQNVNKMLFDDLMSFPNVSGIDAVAVLKRIAQGDTIA
                     DTRDLRNSTGLSYYGYSNLRSYVYYKEPPVKNRLMCDASLLYYTRYFEEGQYDMLHES
                     FLRNDYSATNDITVVVPHLKNNSYWGYFGLDKLDPDIMLKLRMRYGNNYKLGIMHYKA
                     KGEEDLLNIKAEDILADSKWYVGYENSELPGFYNSRLKVYAGNYRATFGEGLVMENTD
                     YYSARKTGFGFSKRILGITPDLSRTLENSLKGGAVEYTTPLFGVTAFVSVDNKDALTY
                     IDKDGYAVMRDEYGNDIYTDRDTTSDYFGRQYYINNNGEHIYNYADSTAVQGDKTKVF
                     ALINPTLRYDDDTMREAEAYFNNEISAGVPYAMNYINLAARKDVLQEKLWGTHLQINP
                     FLGTKLGFTTYTAIYDDAHFVVPTYNNLLPTVLRDSYYYSKLVKNMNAEISNLYSTQT
                     NKYTRDYRRVIGFDGQTVIGNTSIQGEYAELTVNGDDWKLGDDPKAYLVSAHTQFENL
                     YFITLFRNYDVDFDNPYSNSFSEHERFEDTILEKNVYALTNPTIADLYLNSNQSQPER
                     GIYFETRYKFNRYYTINRSYLDIWERLTDGRRSVRFQSELEFRPIYQLAMRLRYKNQV
                     NRYDDDAERGVSKTNEYTLAFRTFLSNRDFLELEYRYNTVCGPPYTSLTYPAAEGENS
                     MAAAQTLMIGDYVAVNYTHYFNKSLKMQGSFLYWFGHGISHWDWEDMEIDFMGEKGSK
                     AWIAFTSRVSQNMYLNLKFKNKTYQDKEIRIRNYNLSDDPTLLDDPVYFQRVEHSENT
                     IRLSIDYRY"
     gene            3004..4431
                     /locus_tag="PLW08_06645"
     CDS             3004..4431
                     /locus_tag="PLW08_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52314.1"
                     /translation="MLSNKSLILICLLIVASALSAWSITDTYFGNPFSTFDARSFAMG
                     GAGLYNSKGAFGIADNPANLTLMRKTLGFSVNTYLNRNEDNRSIPLYNSFDNYIDDSV
                     YSSNINTFDNYALSAFFAHRFGERGLGLGFYYKPVSSFDADYREEVRNNRNTDNDVYP
                     EKIALNKIESEGSLNKTAFVTSFAYSLSDYVDLNLGFEYALLNGDVNREKTIRWTDWA
                     ISQVGEYHLPELTEIDDYELSGDQFKIGASALLSKRFGLAATLTPGTTLDKKGSYYYK
                     RDAYRNTAVDSINTAIKEDYKLPTQIRFGFVYTPRNVVRTEFNMDIEYVAYSEINKRY
                     DDVMNFYVGVEHQVVNFLPLRLGFQAVNNWYFTTEEGTDENNNPITLYHTTKILTPMI
                     TGGSSIKLMDNLKIDLGFGYTWREYEAVDMFGDAYYNDKQYTGSSSYVLWPNSHITLK
                     NRGWENPDKIRENNISLNAGLNFTW"
     gene            4439..>6057
                     /locus_tag="PLW08_06650"
     CDS             4439..>6057
                     /locus_tag="PLW08_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPL52315.1"
                     /translation="MKRIILFLTTLVLLCTIAKAEELRLDLIFSNDMHGGIDRSEATF
                     INPDFPPLLGGGGSAATLIKHIRSLANDRRDNLLLDAGDIFQGRPVGTITNGKAVVEF
                     MNAIGYDAMTIGNHEYDIPEEKLMETLQLAQFPVLSCNIIDKRTGEIPSYIQPYIVVE
                     RLGLKIGILGFTTTDTEKMSFPENIKNIKFLSEKEAIDKYVKILRNQEKVDIVIVLGH
                     AGLPYDIVPTYLTRYDAQGNPLYSERYAVWGYDAQEIAREVEGIDIFIGGHMHKGIPT
                     PWIDPHTHTMVIQGYAYGSNLGWITLKIDPETKTVTGYEVPAVREGSMVTLFEDEFIP
                     DEEIGEMIAEQVAIAEEGMDEVVGYAGVYLSRTNVDAQSLIGNTIVDAMRAEVGADFA
                     FLNLGGVRADIKAGPVTYRDIFQVMPFDNMIVSFRCNGEFLRRIIETRVEGSRHGLII
                     SGGKVVYSKKRENFDRVTHFEIGGKPWDPNKIYTVATTDFIMQGNAGLTMLIQVPQED
                     IIYHQINLRDAIVHYFQKNSPIMTKIDDRWVR"
     assembly_gap    6058..6157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6192..9272
                     /locus_tag="PLW08_06655"
     CDS             6192..9272
                     /locus_tag="PLW08_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL52316.1"
                     /translation="MSPITIVEYHPSYAKAIAEMWNNSSEGWNGRVFNSTEEKVLQQE
                     SGTQYLNLYLAVENEKVIGYAKLTRYVEEKGVAYIEMLSVLPSYHGKGIGKELVQKCV
                     LRAAELGYERIDLFTWSANLKAVPLYKKCGFFWERMETQVTHLMNFLPGLLNNELLKS
                     YWNYFHWYNDLKRELNIEPDGRTDNGFDFYDYLWEKDGKRLEVTFERFGRGIVYIKTP
                     DFSIKVDIPNAKPVFGLTYPVHYTLENYQERPITISLQSENDPLVEYNYQQNIVLAEK
                     AELDATFYLKPLERQLTEWEKCPSVNTRICIEGKEITFRTGIKVQFPLTVELRTRDSV
                     FLPERNYKMFLNVHNHFPVTCFYHLEFPADERLQLSPSKFDFVLNANQKSFIELDFSL
                     NKGLIYNPHLQIIAKPESGKEMQFTTSCYSCFQMLGAKDGIDSPDFIQLLNGNNILYI
                     SKIGEHNFATLVNISTDDLYFPAPEPGKPYSSELKRELPYETVIEKTEDAIQAILKFR
                     STDFPGLDYGIVYRLYDSGLVEYFVTVYALPETEADSWLKIRFYPSKYNLAFAYKNKL
                     YQVGNDLPDVFESNFPQDGFAENWMFIQGVNYTQALIWHPNLTIKPERYYFYGEINLS
                     ELVKSGKNTSAPIRLYQNVFLSPWQVRDLALGRKVPEVIYPTLNLIANQGNPFFTIPC
                     PVEISQILDIEPMGVYSLFSSCLKDAQPQEMQRNEIGERKCSWELNKKPHKPWELLTC
                     KSELYNTVIERKQLIFFSEGEVKKQEKDKLLIADNGCLQITAAKDAQIPGIISLKQKG
                     IEWLENSYPEYAPRSTFNPFAGGIIIKPYAVDAGVLKSESHQVDFIALKDNYGNCWQG
                     LTITTTIDKFEPLKGYIYRQYYLLRPGVPVLAIFAEVVASTGTVGYHTFNFYFHRKHQ
                     QGENDGLFYIQHEDNSWQKIYQTKVQYDFGLDYPTIAMQVADSNYFLQLFKLRRFSTG
                     WVYMDPFIAMLSTYIYTELATIMPTRTEPIFIVMSDELYQKEWLNQLLDISFPV"
BASE COUNT         2893 a         1563 c         1897 g         2929 t
ORIGIN      
        1 ctaataacaa atattttgaa attccaattt gattttggca gacaggaaat aaaaagaatt
       61 attcttaata gcttggtaaa ccagtattaa aaacaatgta atctctaaat cagaaaaagc
      121 aaagcaaaaa aaactggaca gaattatagc tattcctatt gtggctatct atgataaaaa
      181 ataaagtttc agaaagtata aaacttataa ggtagagcaa tgaagaagtt tttaataatg
      241 ctctttgttc tggcggggct atctgtttta acagccaagg tggatttaaa tacagcatct
      301 ttggctgaat tgatgcagtt acccataaca gaaaagcagg caaaagatat ctatgaatat
      361 cgtgaatttg tgaagatatt tgacgatatc tatgagctaa ggaaaattcc ctctattgat
      421 caggagaccc ttgaccgttt aaaaccctta gttgttgttt ctatttatgt agaaacggat
      481 gaagcagcag tccgccgtga agaaatcagg gatttactgg agcgtgtaga cagtaatgaa
      541 ggtgcttcgg aaggaatggc agatgtttgg gaagattatt taatgactcc tcagaatgtg
      601 aataaaatgc tctttgacga tttgatgagt tttcccaatg tttctggaat tgatgctgta
      661 gctgttttaa aaagaattgc ccagggtgat acaattgccg atacaaggga cttacgcaat
      721 tctacaggcc tttcctatta tggttattca aacttgcgca gttatgtata ttataaagag
      781 ccaccggtaa aaaacagatt aatgtgtgat gcttcgcttt tatattatac ccgctatttt
      841 gaagaagggc agtatgatat gttgcacgaa agttttctgc ggaacgatta cagtgctaca
      901 aatgatatca ctgttgtagt tcctcatttg aagaacaatt cctattgggg ttattttggt
      961 ttggataagc ttgatcccga tattatgctt aagctgagga tgcgttacgg caataactat
     1021 aaactgggaa taatgcatta caaagccaaa ggagaagagg atttactgaa tatcaaggca
     1081 gaggatattt tagccgattc caaatggtat gtaggatatg aaaatagcga attgcctggt
     1141 ttttataatt cccgcttaaa ggtttatgcg ggaaactacc gagctacttt tggagagggt
     1201 ttggtgatgg aaaatacgga ttattacagt gcccgtaaaa ccggttttgg tttcagcaaa
     1261 aggattttag gcataacccc cgacctttcc agaacgctgg aaaattcttt aaaaggcgga
     1321 gccgttgaat atacaactcc tttgtttgga gttactgctt ttgtatccgt tgacaataaa
     1381 gatgctttaa catatattga taaagatggt tatgctgtta tgcgggatga atatggaaat
     1441 gatatttata ccgatagaga tacaacatct gattactttg gcagacaata ttatataaat
     1501 aacaatggag agcatattta taattatgca gatagcactg ctgttcaggg agataagacc
     1561 aaggtctttg ctttaatcaa tcccactttg cgttatgatg atgacacaat gagagaagca
     1621 gaagcatatt tcaataacga aatttctgct ggcgtccctt atgcaatgaa ctatatcaat
     1681 cttgctgcac gtaaagatgt tttgcaggag aaattatggg gaacgcattt gcagatcaat
     1741 ccgtttttag gcaccaagct tggatttact acttatactg ctatttatga tgatgctcat
     1801 tttgtggttc ctacctataa taacttgcta ccgacggtct taagggattc ctattattat
     1861 tccaaactgg tgaaaaatat gaatgccgaa atcagtaatc tttatagcac tcagaccaat
     1921 aaatatactc gcgattatag aagagtaatt ggttttgatg gacaaaccgt gattgggaat
     1981 acttccattc aaggtgaata tgctgaactt actgttaatg gggatgattg gaaacttggg
     2041 gatgacccta aggcatatct tgtttctgca cacacccagt ttgaaaatct ctattttatt
     2101 accttgttcc gtaattatga tgtggatttt gataatcctt acagcaacag tttttcagag
     2161 catgaacgct ttgaagatac cattctggaa aagaatgttt atgctttaac taatcctaca
     2221 attgcagacc tttatctcaa ttccaatcag tcacaacccg aacgaggcat ctattttgaa
     2281 acccgttata aatttaacag atactatacc atcaatagaa gctatttaga catttgggaa
     2341 cgcttaaccg atggacgcag aagtgtaaga tttcaaagcg aactggaatt tcgtcctatt
     2401 tatcagcttg ctatgcgttt gcgttataaa aatcaggtta atcgttatga tgatgatgcg
     2461 gaacgaggcg tttccaaaac taatgaatat actttagcat tcagaacttt tctttccaat
     2521 cgtgattttc tggagctgga atatcgttat aacacagttt gtggtccgcc ttatacttcc
     2581 ttaacctatc ctgcggctga aggtgaaaat tcaatggctg cagctcagac cttaatgatt
     2641 ggtgattatg tagcggttaa ttatactcac tatttcaata aatcgttaaa aatgcaaggt
     2701 tctttcctgt attggtttgg gcatggcatt tcgcattggg attgggaaga tatggaaatt
     2761 gattttatgg gtgaaaaagg ttctaaagca tggattgcct ttaccagtcg cgtttcccaa
     2821 aatatgtatc taaatttgaa attcaagaat aaaacctatc aggataaaga aatccggatt
     2881 cgtaattata atctttcgga tgatcctact ttattggatg atcctgtcta ttttcaaaga
     2941 gttgaacaca gcgaaaatac cattcgctta agcattgact accgttatta atatggagga
     3001 ataatgttat ctaataagtc cttaatactt atatgtctat taattgttgc ttcagcgctt
     3061 tctgcctgga gcataaccga tacctatttc ggcaatcctt tttccacttt tgatgcccgc
     3121 agttttgcta tgggcggagc aggtttatac aatagtaagg gtgctttcgg catagcggat
     3181 aatcctgcca atttaacttt gatgcggaaa acccttggtt tttccgttaa tacttatctg
     3241 aatcggaatg aggataatcg cagtattcct ttatataatt cctttgataa ctatatagac
     3301 gattctgtgt attcctctaa tatcaatacc tttgataatt atgctctctc tgcttttttt
     3361 gcacatcgtt ttggggaaag aggtttgggt ttgggtttct attataaacc cgtttccagt
     3421 tttgatgccg attatagaga agaagttcgc aacaatcgca ataccgataa cgatgtttac
     3481 ccggaaaaaa ttgccttaaa caaaatagaa agtgagggtt ccctgaataa aacagctttt
     3541 gtaacctcct ttgcttatag cttaagcgat tatgttgatc tgaatttagg ttttgaatat
     3601 gctttgctga acggtgatgt aaacagggag aaaactatcc gttggaccga ttgggctatc
     3661 agtcaggtag gagaatatca tcttccagaa ttaactgaga ttgatgatta tgaactaagt
     3721 ggcgatcaat tcaaaatagg tgcttcagct cttttgagta agcgttttgg tttagctgca
     3781 actttaactc ccggaaccac tttagataaa aaaggcagtt actattacaa aagagatgct
     3841 tatcgtaaca ctgccgtgga tagtatcaat actgctatca aagaggatta taaacttccc
     3901 actcaaattc gttttggttt tgtatacact cctcgtaatg ttgtgcgcac tgaattcaat
     3961 atggatatag aatatgttgc ctacagcgaa attaataagc gttatgatga tgtaatgaat
     4021 ttctatgtcg gagtggaaca tcaggtagtc aactttttac ctttgcgtct tggttttcag
     4081 gcagtaaata actggtattt tactaccgaa gaaggcacag atgaaaataa caatcctata
     4141 actttatatc atacaactaa aatattaaca cctatgataa ccggtgggag cagcatcaaa
     4201 ctaatggata acttgaaaat tgatctcggt ttcggttata cctggcgtga atatgaagcg
     4261 gtggatatgt ttggtgatgc ttattataat gataaacaat atactggctc ttcaagttat
     4321 gttttatggc ccaattccca tataaccctg aaaaatcgtg gttgggaaaa tcctgataaa
     4381 attcgggaaa acaatatttc cctgaatgcc ggacttaatt ttacctggta aaaaaactat
     4441 gaaacggata atattattcc ttactacatt agttctgctt tgcacaattg ccaaagcgga
     4501 agaactgcgt ttagacctca ttttttctaa tgatatgcat ggtggtatag accgttcgga
     4561 agcaactttt atcaatccgg attttcctcc acttcttgga ggaggaggtt ccgccgctac
     4621 tttaattaag cacattcgtt ctttggcaaa tgacagaaga gataatttac ttttggatgc
     4681 gggtgatatt tttcaaggtc gtcctgtagg aaccataact aacgggaaag ctgtggttga
     4741 atttatgaat gccattggct atgatgcaat gactattggc aatcacgaat atgatattcc
     4801 tgaagagaaa ttgatggaga ccctgcagtt agctcagttt ccggtattat cgtgtaatat
     4861 tatagataaa agaacaggtg aaattccaag ctatatccag ccctatattg tggtagagcg
     4921 attagggtta aaaatcggga ttttgggttt tactacaacc gatacggaaa agatgagctt
     4981 tccggaaaac atcaaaaata ttaaattcct ttccgaaaaa gaagcgatag ataaatatgt
     5041 aaaaatcctc cgtaatcagg aaaaggtgga tattgttatt gtcctggggc atgccggttt
     5101 accttatgat attgttccta cctatcttac ccgttatgat gctcaaggaa atcctttata
     5161 cagtgaaaga tatgctgttt ggggatatga tgctcaagaa atagcgcgcg aagtggaagg
     5221 catagatatt ttcattggcg gccatatgca taaaggaatt cctaccccct ggatagaccc
     5281 ccacacacat acaatggtta ttcaaggtta tgcttacggt tccaatctgg gatggataac
     5341 cttaaaaata gatcctgaaa caaaaactgt aaccggttat gaagttcctg ccgttcgtga
     5401 aggaagtatg gttacccttt ttgaggatga atttattccc gatgaagaaa ttggagagat
     5461 gattgctgaa caggttgcca tagcagaaga aggaatggac gaagttgtgg gttatgcagg
     5521 tgtttatctt tcccgcacta atgtagatgc tcaatcctta attggaaata ccattgtaga
     5581 tgctatgcgt gctgaagttg gtgccgattt tgcctttctg aatttgggag gagtgcgtgc
     5641 cgatattaaa gcaggtcctg ttacctatag agatattttt caagtgatgc cttttgataa
     5701 tatgatagtt tcttttcgtt gtaacggtga atttttgcgc cggataattg aaacccgagt
     5761 tgagggctca agacatggct taattatctc tggaggcaag gttgtctatt ccaaaaagcg
     5821 ggaaaatttt gaccgcgtaa ctcattttga aattggagga aaaccctggg accccaataa
     5881 aatttatacc gttgctacta cggattttat tatgcaaggt aatgcagggc tcacaatgct
     5941 gattcaagtg cctcaagaag atattatcta tcaccaaatc aatttacggg atgcaattgt
     6001 gcactatttt cagaaaaata gcccgattat gacgaaaata gatgatcgct gggtgcgnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta tattcattct tcattcttta
     6181 ttggagttta tatgtcccct ataacaatag ttgaatatca tccttcctat gccaaagcca
     6241 ttgctgaaat gtggaataac agcagtgagg gttggaacgg cagagttttt aacagcacgg
     6301 aagaaaaggt gttacaacag gaatccggca cccaatatct gaatttgtat ctggcagtag
     6361 aaaatgaaaa agttatcggt tatgcaaaat taactcgcta tgtagaagaa aaaggagttg
     6421 cctatattga aatgctctcc gttttacctt cgtatcacgg aaaaggtata ggtaaagaat
     6481 tagtccaaaa atgtgtattg agagctgcgg aattgggtta tgaacgcatt gatttattca
     6541 cctggtctgc caatttaaaa gctgttccgc tttataaaaa atgcggcttt ttctgggaaa
     6601 gaatggaaac tcaggtaacg catctgatga attttcttcc cggtttgctc aataatgaat
     6661 tgctaaaatc atattggaat tattttcact ggtataatga cctgaaaaga gaattgaata
     6721 tagagccaga tggcagaact gacaacggct ttgatttcta tgattatctt tgggagaagg
     6781 atggtaaacg gttagaagtt acttttgagc gtttcggtag agggattgta tatatcaaga
     6841 ccccggactt ttctataaaa gtagatatcc caaatgccaa accggttttt ggcttaactt
     6901 atcctgtgca ttacactttg gaaaattatc aggaaagacc gattactatt tctctgcaaa
     6961 gtgaaaatga tcctctggtg gaatataatt atcaacagaa cattgtgtta gcggaaaaag
     7021 cagaattgga tgccactttt tatctgaaac ccttagagcg tcaattaacg gaatgggaaa
     7081 aatgtccttc tgttaatacc cgtatctgta ttgaaggcaa agagataacc tttagaaccg
     7141 gcataaaagt gcaatttccc ttaacggttg aattgcgtac ccgggacagt gtttttcttc
     7201 ctgagcgtaa ttataagatg ttcttgaatg tccacaatca ttttccggta acctgttttt
     7261 atcatcttga atttcccgct gatgagcgtt tacagcttag cccaagcaag tttgactttg
     7321 ttttaaatgc taatcagaaa agctttatag agcttgattt ttcccttaac aagggcttaa
     7381 tttataatcc gcatctgcag attattgcca aacctgaaag tggaaaggaa atgcaattta
     7441 ctaccagttg ctattcttgc tttcagatgt tgggtgccaa agatggaatt gattcacctg
     7501 attttataca acttttgaac ggaaacaata tcctctatat atccaaaatc ggagaacata
     7561 actttgccac attagtaaac ataagcactg atgatttata ttttccggca cctgaaccgg
     7621 gaaaaccata ttcctcagaa ctgaaaagag agctgcctta tgaaacggtt attgaaaaaa
     7681 cggaggatgc cattcaggca attttgaagt ttcgttcaac tgattttccg gggcttgatt
     7741 atggaatcgt ttatcgtctt tatgatagtg gtttagtaga atattttgtt actgtgtatg
     7801 ccctacctga aaccgaagca gacagctggc taaaaataag attttatcct tccaaataca
     7861 accttgcttt cgcttataaa aacaaactct atcaggtagg aaatgatctc cccgatgttt
     7921 ttgaaagtaa ttttcctcag gatggctttg ctgaaaactg gatgtttatt caaggtgtta
     7981 attatactca ggcacttatc tggcatccca atctcactat taaaccggaa agatactatt
     8041 tctatgggga gattaatctt agtgaattgg ttaaaagtgg aaagaatact tccgctccga
     8101 ttcggctcta tcaaaatgtt tttctttctc cctggcaggt tcgggatctt gctttgggaa
     8161 gaaaagttcc cgaagttatc tatcctaccc tgaatttaat tgctaaccag ggaaatccct
     8221 tttttactat accctgccct gtagaaataa gccaaattct ggatattgag ccaatgggag
     8281 tttacagttt attttccagt tgtctaaagg atgcacagcc ccaagaaatg caaagaaatg
     8341 agatagggga aaggaaatgt agctgggaat taaacaaaaa accgcataaa ccctgggaat
     8401 tgttaacttg caaaagcgaa ttatataaca ctgtaataga acgcaaacag cttattttct
     8461 tttccgaggg tgaagttaaa aaacaggaaa aggataaact gctaattgct gataatggct
     8521 gtttacaaat tactgctgcc aaagatgctc aaatcccagg cattatctct ttgaagcaga
     8581 aaggaattga atggctggaa aacagttatc ctgaatatgc acccagatcc actttcaatc
     8641 cttttgccgg aggaataatt ataaaacctt atgctgtaga tgctggtgta ttaaaatcag
     8701 aaagccatca agtggatttt attgctctta aagataacta tggaaattgc tggcagggac
     8761 taactattac gaccacaata gacaaatttg aaccgctaaa gggatatatt taccgacagt
     8821 attatttatt gagaccagga gttccggttt tagctatttt tgctgaagtt gttgcttcca
     8881 caggaactgt tggatatcat acttttaatt tttattttca tcggaaacat cagcagggtg
     8941 aaaatgacgg cttattctat attcaacacg aagataacag ctggcagaaa atttatcaga
     9001 caaaggttca gtacgatttc gggttggatt atccaaccat agcaatgcag gttgctgata
     9061 gcaattactt tctgcaatta tttaagttaa gaagattttc taccggttgg gtatatatgg
     9121 acccctttat agcaatgtta agcacttata tttataccga attggctact ataatgccga
     9181 caagaacaga gccgatattt atagtaatgt ccgatgaact gtatcaaaaa gaatggctta
     9241 atcagttgct ggatattagc tttccggttt aatttcctgt agataagttt tgttaatagg
     9301 taggggattt tcaggatttt ggggatttac aggattttat tgtataatat tttgagggtt
     9361 gagagggttg aaaaagttga ga
//
LOCUS       DAOOZV010000066         8482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_7777_length_8482_cov_84.430877, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000066 DAOOZV010000000
VERSION     DAOOZV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_8482_cov_84.430877"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1311
                     /locus_tag="PLW08_06660"
     CDS             280..1311
                     /locus_tag="PLW08_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPL52317.1"
                     /translation="MQTIDALQIMFLVMMLPCLISCMQAKGNVYPLDSKYVKAEELNA
                     KLDKIVQIKPELAEKRIIGFSSAENLPVYALQLGNKNAEKAILVIGQHHGEEVIGVNV
                     SLALAEKLLNNYSQEDKQSHLLENYQVWIIPTLNPEGFRIVSSGILRTKRKNNRDTNN
                     NHKLDLRTDGVDLNRNYPIFWDMDSETEINSPYYKGSEPASESEIQAIIALAQQQNFA
                     LAIFLHSSISGAYSEKIYLPARSNDSELFQKTQTLATIYAKEVKKDYLKGTYEVYAGN
                     TSEVGNARNFFFYRMGTPAFLVEIGGQNKRGQSVIHPANAMLEKIVNKNVQAMLKVFE
                     ELEKMKKEK"
     gene            complement(1494..3233)
                     /gene="rpoD"
                     /locus_tag="PLW08_06665"
     CDS             complement(1494..3233)
                     /gene="rpoD"
                     /locus_tag="PLW08_06665"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPL52318.1"
                     /translation="MITYNECLKKLVELGKESGYITFKQINEILPPSPFFLDKVDDII
                     FDLSQDGIEIIDETEKRITKSGAAIRKPSTAKKFKSKRYYDDPVRMYLREMGRVPLLD
                     REGEVRVAKKIETYQKMINQNVFKCGSTLREMYNFLHRYRERRVRLDQIFKVDISTWI
                     DKNQDAKIIEKFKEILKENEEKFEKVGNILDNCEFDDTGTSSRQEEIDELRSQIVNTF
                     MQLSYNDKLIKRMVYRIRSLVERIEESYESINNLTRIKRYTIDEICMYGRRAKKSDED
                     FQEVLEETGIDPNVFVETLRKIKNARRKIRRVELETKMTGNELIFLLREIDRAERNKE
                     RAQNEMIEANVRLVISIAKHYNNRGLEFLDLIQEGNTGLMRAVEKYDYRKGYKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMIESINKINRTSRRLMQKLGREPYPEEISEVLDI
                     PVEKIKNIMSISKEPVSLDKPIGHDSEDSILGDFIEDRTIISPERLAERSLLKKQVDE
                     VLKTLTPREERVIRLRFGIDDGYHRTLEEVGNIFQVTRERIRQIEDKALKKLRHPSRA
                     AILQQFLDNFHVK"
     gene            complement(3256..4977)
                     /gene="dnaG"
                     /locus_tag="PLW08_06670"
     CDS             complement(3256..4977)
                     /gene="dnaG"
                     /locus_tag="PLW08_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPL52319.1"
                     /translation="MDQNLIDQIRRANDIVDVIQGYLPLKRVGSNFRGLCPFHNDSRP
                     SLYVSQSKQIFKCFACGKAGNVIGFVADFEKLTFIEAVKKLAHRAGIQVPEYEKTKVV
                     NTKREQLLSVYKSAAEFFTSSLFAFGQNVLDYLKKRSFSPETAKELQLGYALNSEKAL
                     LNHLLKEGYSVSLLKDSGLFGNYGGGLTDLFKDRLMFPIHNSLGEVIAFGGRILEPKE
                     GVGKYINSPGTELYTKGKELYGLFKTKYNISKAGTAIICEGYFDFLRLYENGFTNSVA
                     SLGTALTEDQINLLARFCNRVIMLYDGDSAGIKAAVRAGLLCLSRGMEVLIANLPAEE
                     DPDSLILKQGKMAMQEVVDKSIPLINFLATDPRPEQPTAERIELILDALRILKDRIKQ
                     ELLLQEVSDAFGITVGALNSKLRRSSVSVSAESSEQTVPQYESFEERNVLALALKDYE
                     SYKLLANDLDSSYFNNKRYRQVYNFLITQNLKSEPWEPAALLDNIENNEIKESLAELL
                     FEDLQQMCFEDCLTGLRIRKVQRDLEEMDRAISKDPKNMELLKEKEKLALKYRQMTRK
                     VVNKVLY"
     gene            complement(5041..7383)
                     /locus_tag="PLW08_06675"
     CDS             complement(5041..7383)
                     /locus_tag="PLW08_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPL52320.1"
                     /translation="MTYHNPDLEFETLVAQIGKRCHSDLGKEQASLLQPLSDLGEIIK
                     SQKLVAEIQEQLLRGRDYDFSGLTNLQPLFEDSRNTLFAFEEFSAVYANNLIAEEILA
                     QLKLWEEFPEITLYLKKLTSFSFLNSRFLEIFDKDGEILDTASKELATIRKRANSLRM
                     QIQKTMQALLSDSSLEHYLQDKYVTQREDRYVLPVKENAVPFVNGIVQSHSGSKSTVF
                     IEPMSVVPLNNELQLIFQQEKQEIYRIFSAYTSAIKAEKKGLLRNQKILAWLDFRFAC
                     GRLCNTIKAKTPIMLSRPCLNLKSARHPLLILRLGNCRKVIPFDLELGEEKKIIILSG
                     PNTGGKTVLLKAVGLITLMALSGLPVPADADSEIGFFSNVFADIGDDQSIENALSTFS
                     AHLEKIGKMLNKAKPDTLVLVDEIGAATDPQQGSALAQAILEKFLALGCPAIVTTHYT
                     ALKIFAEQHPRCLNASMQFDTKNLQPTYKFIAGFPGDSFAIEVAASLGIDPALIERAK
                     ELAGNQNVQFTELLKKMQDEKKKYSVSCYQNELKTHLLETKLQELAKKEEEWEKEVKI
                     RRQNHLKELQQELIAQQKIYQNDLAELKTLSKQERKTFSEYKLHNIAVKNAEIQKELD
                     KDLSANNLPVSNPQPGMKVWLSNFETEAVILSIEGEQVKVDMNGITFKTPVSTLFEPK
                     TKTETTKIEPKVKANVAPVAKFELKIIGLTFEEAKPLIDEFLDNAALAGLHSLRIVHG
                     KGTGALRTKVRDYLKKRKEVKGVGTPSPNEGGTGVTIVTI"
     gene            complement(7545..8099)
                     /locus_tag="PLW08_06680"
     CDS             complement(7545..8099)
                     /locus_tag="PLW08_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPL52321.1"
                     /translation="MGFIDWIILAVLILFTFFGWRKGLLASLVQLGGYILTFFLVGHY
                     YPLVQRNLMQKFHFAKWLATIVSVVLIIVLIVVIVRLVIYILERFLRALKLSAVNHTL
                     GAVLGIINGLLVVIILTVVLDFIPKLSTPLKNGEKHRVYAGVDLVKEEIFTKLKLTER
                     TKLIKMPKLPFAEKLNGEKVKSEK"
     gene            complement(8191..8397)
                     /gene="rpsU"
                     /locus_tag="PLW08_06685"
     CDS             complement(8191..8397)
                     /gene="rpsU"
                     /locus_tag="PLW08_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPL52322.1"
                     /translation="MPTVVAKENESFDLLLKRFKKKCEKAAILSEIKKNQAYEKPSVR
                     KKREANVARRKMLKMQRRMQRYLK"
BASE COUNT         2423 a         1683 c         1633 g         2743 t
ORIGIN      
        1 caaaattaga aggaaaaaaa attgaagatg aatccaaata taagattgac agaatcagat
       61 aaaatatttt atgagttgac aagtagcttg ctgtaatata atagaaagca agagaataaa
      121 agaaagctaa aatgtataag tatgtgcctt ccactgaaat tcattcttca tttctttggc
      181 tcttattgta gaaccctcac tgctttagct ctcactttgc aattctcggt ttctgtattt
      241 ctccttgacg ataagttcat ttctgataaa aaggtattta tgcaaacaat agatgcttta
      301 caaataatgt ttctggtgat gatgttacct tgcctgatat cttgtatgca ggcaaaagga
      361 aatgtatatc ctcttgattc caaatatgtt aaggcagaag aactgaatgc taaattggat
      421 aaaatcgtgc aaattaaacc agaacttgct gaaaaaagga ttattggttt cagcagtgcc
      481 gaaaatttac ctgtttatgc cttgcaatta gggaataaaa atgccgaaaa agcaattttg
      541 gtaataggtc aacatcatgg agaagaagtt attggcgtta atgtttccct tgctttagcg
      601 gaaaagttgt taaacaatta ttcccaagag gataagcaat cccatctttt agagaattat
      661 caggtttgga ttattcccac tttgaatccg gaagggtttc ggattgtaag ttccggaatt
      721 cttagaacta aacgtaaaaa taaccgggat accaataata atcataaact tgatctgcgc
      781 acagatggag tggacttaaa tcgtaattat cctatttttt gggatatgga ctcggaaaca
      841 gaaatcaaca gtccctatta taagggctcg gaaccggctt ccgaaagtga aatacaagca
      901 attatcgctt tggctcaaca gcaaaacttt gctttggcaa tttttctgca tagttccatt
      961 tccggtgctt acagcgaaaa aatatatctt cctgcccgga gtaatgattc agaacttttt
     1021 caaaagacgc agaccttagc cactatttac gctaaagaag tgaaaaagga ctatctgaaa
     1081 ggaacttacg aggtctatgc aggaaatact tccgaagtgg gaaatgcgcg taattttttc
     1141 ttttatcgga tgggaactcc ggctttttta gtggaaatcg gaggccaaaa taaacgcgga
     1201 caaagcgtta ttcatcctgc caatgctatg ctggaaaaga ttgtgaataa aaatgtgcag
     1261 gcaatgctga aagtgtttga agaactggaa aaaatgaaga aggaaaaata gatgtaagtc
     1321 aggagtcgtc actgccttta atattttttt acatttttct ctatcaaatt tggcagtgag
     1381 gactcgtgac gacacagtta cctgaaacaa gattgcaact caaattattg ccttgtttca
     1441 ccttttccct ttttcactac aaaaatgcgg aggaatggcg tctcctggct acattatttc
     1501 acatggaagt tatccagaaa ctgctgtaaa attgcagccc gggaaggatg gcgaagtttt
     1561 ttcagtgcct tatcttcaat ttggcgaatg cgttcacggg taacctgaaa aatattgcca
     1621 acttcttcca gggttctatg atagccatca tcaatgccaa aacgcagacg aataactcgt
     1681 tcctcgcgcg gagttaatgt tttcagaact tcatccacct gttttttcag gagggaacgt
     1741 tctgccaggc gttctggaga aataattgtt ctatcctcaa taaaatcccc taagatacta
     1801 tcttcgctat cgtgtccaat gggcttatcc aaagaaacgg gctctttgct gatggacata
     1861 atgtttttga ttttttccac agggatatcc aagacctcgg aaatttcttc aggatagggt
     1921 tcacgcccca atttttgcat caggcgacgg ctggttctat taattttatt gatggattca
     1981 atcatatgta caggaatacg gatagttctt gcctggtctg caatagctct ggtaattgcc
     2041 tgacgaatcc accaggtggc ataagtgctg aatttatagc ccttacggta atcatatttt
     2101 tctacggctc tcatcagtcc ggtatttcct tcctgaatga ggtccaaaaa ttccagtcca
     2161 cgattgttat agtgtttagc gatactgatt acgaggcgga catttgcctc aatcatttca
     2221 ttttgcgctc tttctttatt gcgttcggca cggtcaattt cccttagcag gaagattaat
     2281 tcgttaccgg tcattttggt ttccaattct acgcgacgga ttttcctgcg ggcattttta
     2341 attttgcgca aggtctctac aaaaacattg gggtcaattc ctgtttcttc caaaacttcc
     2401 tgaaagtcct catcgctttt tttggcacgg cgtccataca tacaaatttc gtcaatggta
     2461 tagcgtttaa tgcgggttaa attattgatg ctttcatagc tttcttcaat gcgttcaacg
     2521 agggagcgaa tacggtaaac cattcgttta atgagtttat cgttatagga cagttgcata
     2581 aaggtattca caatttgaga gcggagttca tcaatttctt cctgtctgct ggaagttccg
     2641 gtatcgtcaa attcacagtt atccaggata tttcctactt tttcaaattt ctcttcgttt
     2701 tctttgagga tttctttgaa tttctctatg attttggcat cctgattttt atctatccaa
     2761 gtgctgatat ccactttgaa aatttggtct aaacgaactc tgcgttcacg atagcgatgc
     2821 aggaagttat acatttccct taaagtgctt ccgcatttga acacattttg gttgatcatt
     2881 ttctgatagg tctcaatctt tttggcaaca cgcacttctc cttctctatc cagaagggga
     2941 acacgaccca tttctcgtaa atacattcta acggggtcat cgtaatagcg tttggacttg
     3001 aattttttag cggtggatgg tttccgaata gcggctccgc ttttagtgat gcgtttttcg
     3061 gtttcgtcta tgatttcaat tccatcctgg gaaaggtcaa aaataatatc atccacctta
     3121 tccagaaaga agggactggg gggcagaatt tcatttatct gcttaaaggt tatgtaaccg
     3181 gattctttcc ccaattccac cagttttttc aaacattcat tgtaagtaat cattaatgct
     3241 ccttggaaaa accctctaat agaggacttt gtttacgacc ttcctggtca tttgcctata
     3301 ctttagagcc agcttttctt tctctttgag gagttccata tttttagggt ctttgctaat
     3361 tgcgcgatcc atttcttcca agtccctttg gactttacgg atgcgtaagc cggtgaggca
     3421 atcttcaaag cacatttgct gtaaatcctc aaaaagaagt tcagctaagc tttcctttat
     3481 ttcattattt tcaatattat caagcagggc tgccggttcc cagggctctg actttagatt
     3541 ttgagtgatc aggaaattat atacctgacg gtagcgctta ttattaaaat aactggaatc
     3601 caagtcgttg gcaagtaatt tataagattc atagtccttt agtgccaaag caagaacatt
     3661 tcgctcttca aagctttcgt attgaggcac agtttgttca cttgattcag cggaaacgga
     3721 aacactgcta cggcgtaatt tactgtttaa agcacctaca gtaattccaa aggcatcgga
     3781 gacctcttgc agtaatagct cctgtttaat tctgtccttt aatatcctta aagcatctaa
     3841 aatcagttca attctttccg cggttggctg ttccggacgc ggatcggttg ccaggaaatt
     3901 gattaaggga attgatttat ctacaacttc ctgcattgcc attttaccct gttttaagat
     3961 cagggaatcg gggtcttctt ctgcaggcag attggctatt aaaacttcca ttcctctact
     4021 taaacataaa agtccggctc ttactgcagc tttaattcct gcactatcgc catcatataa
     4081 cattataacc cgattacaga aacgtgccag taaatttatt tgatcttccg ttagagctgt
     4141 tcccaagctg gcaacggaat tggtaaagcc gttttcgtat agacgcagga aatcaaaata
     4201 accctcacag ataatagctg tgccggcttt gctgatattg tatttggttt tgaataaacc
     4261 gtaaagttct ttgcctttgg tatataattc cgttccgggt gaatttatgt attttcccac
     4321 gccttctttg ggttccagaa tgcgtccgcc gaaagcgata acttccccta aactattatg
     4381 aatagggaac atcaaacggt ctttaaagag gtctgttaaa cccccgccat aattgccaaa
     4441 aagtccggag tcctttagca gagaaacact ataaccttct ttcaggagat gatttaaaag
     4501 cgctttttca ctgtttaaag catagcccaa ctgaagttct ttagctgttt cgggagaaaa
     4561 agaacgtttt ttcaaataat ccaaaacatt ctgtccaaac gcaaaaagcg aggaagtgaa
     4621 aaactctgcg gcacttttat acacgcttaa cagctgttcc cttttagtat ttacaacttt
     4681 ggttttttca tattcaggaa cttgtatacc agcacggtga gccaattttt ttaccgcttc
     4741 aataaaggtc agcttttcaa agtccgctac aaaaccgatt acattaccgg ctttgccaca
     4801 agcgaaacat ttaaaaatct gtttggattg gcttacataa agcgaagggc gagaatcatt
     4861 atgaaaagga cataaaccac gaaagttact tcccaccctt ttcaagggaa gataaccttg
     4921 aataacatct acgatgtcgt tagcacgacg aatttgatct attaagtttt gatccatttt
     4981 ttctcggaag aacaacatcc ctgttgttca ttagcgttag ccaggaggta aaccttccgg
     5041 ttagatagta acaatggtta ctccggttcc accttcatta ggacttggag ttccgacacc
     5101 ttttacctct tttctttttt tcagatagtc ccgaacctta gttcttaaag cgccggttcc
     5161 ttttccgtga acaattctta aagaatgcaa acctgccagg gcagcattat ccaaaaattc
     5221 atctatcaag ggttttgcct cttcaaaggt tagccctata attttcagtt caaattttgc
     5281 tacgggagca acattagctt tcactttagg ttctattttt gttgtttcag ttttcgtttt
     5341 gggctcaaaa agtgtgctca caggtgtttt gaaggtaatt ccgttcatat ctacttttac
     5401 ctgttcacct tcaatagaca aaataactgc ttccgtttca aaattggaaa gccagacctt
     5461 cattccgggt tgaggattgc tgacgggcaa gttattagcg gaaaggtctt tgtcaagttc
     5521 tttttgaatc tcggcatttt taactgctat attatgtaat ttgtattccg aaaatgtttt
     5581 acgttcctgt ttggaaaggg ttttcagttc tgccaggtca ttctgatata ttttttgttg
     5641 agcgatcagt tcttgttgca attcctttaa gtgattttgg cggcgtattt tcacttcttt
     5701 ttcccattcc tcttcttttt ttgccagttc ctgcagtttg gtttccagca ggtgtgtttt
     5761 cagttcattt tgatagcagg aaacgctgta tttctttttt tcgtcctgca tttttttcag
     5821 cagttcggta aattggacat tttggttgcc ggcaagttct ttggctcttt caattagggc
     5881 agggtcaatt cccagagagg cagcaacttc aatagcaaag ctgtcacctg ggaaaccggc
     5941 aataaattta taggttggct ggaggttttt ggtatcaaat tgcattgagg cattcagaca
     6001 cctgggatgc tgttcagcaa agattttcag ggctgtgtaa tgagttgtaa caattgccgg
     6061 gcaacctaaa gccaaaaact tttccagaat tgcctgtgct aaagcagaac cctgttgagg
     6121 atcagttgca gcacctattt catctactaa aaccagggta tccggttttg ccttattgag
     6181 cattttacct attttttcca ggtgagcgga aaaagtggaa agagcatttt ctatggactg
     6241 gtcatctcca atatcggcaa aaacattgct aaaaaagcca atttcactat cagcatcagc
     6301 aggaacggga agtcctgaaa gagccatcag agtaatcaaa ccgactgcct ttagcagaac
     6361 tgtttttccc ccggtattgg gtccgcttaa aataataatt tttttctctt cgcccagttc
     6421 caaatcaaag ggaataactt tacggcaatt tcccaggcgt aaaatcagca gaggatgcct
     6481 tgccgatttt agattcaaac aaggacggga tagcattatc ggggtttttg cctttattgt
     6541 gttacaaagc cgtccacaag caaaacgaaa atccagccaa gccagaattt tctgattgcg
     6601 taaaagtcct ttcttttctg ctttgatagc cgaagtatag gcactgaaaa tacgataaat
     6661 ttcctgcttt tcctgctgaa aaataagctg gagttcatta ttgagaggta caacagacat
     6721 tggttctata aaaacagtgg atttactgcc ggaatggctt tgaacgatgc catttacgaa
     6781 aggaaccgca ttttctttaa cgggcagaac ataacggtct tccctttgag ttacatattt
     6841 atcttgcaag tagtgttcta aactactgtc ggaaagtaat gcctgcattg ttttctgaat
     6901 ctgcattctt aaggaattag ctcttttgcg gatagttgcc agttctttgg aagcggtatc
     6961 caaaatttca ccgtctttat caaaaatctc caaaaacctg gaattgagaa aggaaaagga
     7021 ggttaatttc tttaggtaaa gagtaatttc cggaaattcc tcccaaagct ttagctgagc
     7081 caaaatttct tcggcaatga ggttattggc ataaacagca gaaaattcct caaacgcaaa
     7141 aagagtgttt cggctgtctt caaaaagagg ttgcagatta gttaaaccac taaaatcata
     7201 atctctaccc cgcaaaagtt gttcctggat ttcggctacc aatttctggg atttgattat
     7261 ttcccccaaa tcggataggg gctgtaataa agatgcctgt tctttaccta aatcactatg
     7321 acagcgtttg ccgatttgag caacgagggt ttcaaattcc aggtcgggat tatgataagt
     7381 cataaattag atagatagcg agatggagaa gtgagaggac tttgtccaat gagagttaaa
     7441 acagtgagaa gaatttgggg gatgggtaaa agttattttg gcagccacga ctcctgactt
     7501 aattttgcac cgaaaattat cactttgttt cactttttca cctttcactt ttcacttttt
     7561 accttttccc cgttcaactt ctccgcaaaa ggcaatttcg gcattttaat caattttgtt
     7621 ctttctgtca gtttcagttt ggtaaatatt tcttctttaa caaggtctac accggcataa
     7681 acgcgatgtt tttctccatt tttcaaaggg gtagaaagtt tgggtataaa atccaaaaca
     7741 acggttaaaa taataactac cagcaaaccg ttgataattc ctaaaacggc acccagagta
     7801 tggttaacag cagagagttt taaagcgcgt aaaaaccttt ccaggatgta aataacaagt
     7861 cgcacaatca ccacaattaa aactatgatt aaaacaacgg aaacaatagt tgccagccat
     7921 ttagcaaagt gaaatttttg cattaaattg cgttgcacca gaggatagta atgacccacc
     7981 agaaagaaag tgagaatata ccctccaagc tgaactaaac ttgccagcag acctttacgc
     8041 caaccgaaaa aagtgaataa gatcaggact gccaggatga tccagtcaat aaatcccatt
     8101 ttttccccgc ttaaaaaagc ttacagggaa taatcttccc tgtagcttat attcttgtaa
     8161 ttagatgaat tctcttcgtt aagaggagtc ctatttcaga tagcgttgca tccgacgttg
     8221 catttttagc attttgcgtc gggcaacatt tgcctcgcgt ttcttacgaa cgcttggttt
     8281 ttcgtatgcc tgattctttt ttatctcgga aagaatagct gctttttcgc attttttctt
     8341 aaatcgtttt aataaaagat cgaagctttc attttctttt gctacaactg taggcaaata
     8401 aatcaccacc tttattttat attttttcta atgccatatt tttaaatgtt gttatgctgt
     8461 caagggagaa aaattaactc tc
//
LOCUS       DAOOZV010000067         8291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_7980_length_8291_cov_57.320787, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000067 DAOOZV010000000
VERSION     DAOOZV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_8291_cov_57.320787"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1647)
                     /locus_tag="PLW08_06690"
     CDS             complement(403..1647)
                     /locus_tag="PLW08_06690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL52323.1"
                     /translation="MNWGKDILLAFIFLLLTTFCLISLMGINPKHPAEIKPDLQKPSE
                     PWIIQTIPEGGSIFSVLANLNLPLKEVGLLAFKFGDYIDVSTIQPGDTLKILLSEDKQ
                     HIAKMVFVQEPTTRHHFTVSADSLVYSLEALPVKRVKRILEGKLEGTLDASLLAMGFA
                     PQDKQAINNGLETEINFARDARNGDFFRVFVEERIFEGKTLPGRKIFYVQYSGERTGT
                     CELFRYEDEEEKSVLNGLYTKEGKSCHSNGIGFPLSVIHLVSPFGRRLDPFYGNWANH
                     QGVDYRAHYGTPVYAVANGKVTDAGYNGGWGNEIRIKHPSGLTTQYAHLSSMSVRKGQ
                     TVKKGQIIGRVGSTGRSTGAHLHFGLIKGKKYINPTNLKMVGTEKLNETQMARFKSQQ
                     QIILQDMENLMHPKPVIASAKK"
     gene            complement(1644..2588)
                     /locus_tag="PLW08_06695"
     CDS             complement(1644..2588)
                     /locus_tag="PLW08_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPL52324.1"
                     /translation="MTDRDNLTRVTVNLGLFSNILLSIVKTCVGILGHSAALLADGIN
                     STSDVVYYIAVKIFMKQAQKPADVEHPYGHRQLESISAIVVGAFILTTGIAIFWESVN
                     KVYDLLSKTEIGQPLSIWTLVIALFTFGLKIFLYTYTRKNIPKTKNPTLKALANDHLN
                     DIMAAVAVVVGVLLGRLGYYWMDPAAGAIVAIYIIKTGVEIIMESSRELMDYLPDEDF
                     VRELKTEAMAVEGVRSIEDLGIHRFGPYFTVNMTITVDGDISVDKGNIISDSVEKRLL
                     DKFSTGLRQVHIHFHPYFEKHSSTDPKPGSKTNIGVNL"
     gene            complement(2593..3588)
                     /locus_tag="PLW08_06700"
     CDS             complement(2593..3588)
                     /locus_tag="PLW08_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52325.1"
                     /translation="MEFSWISCRRYKNIAIVGICKNAGKTTVLNYILAKYPFPWGILS
                     TGRDGETEDTLFKIPKPKVKIPEGCFFCTDAKTLLQHGSGISILTKTSFQSGSRELWI
                     VKSEKYLETEITGPASVSGQIACAELLQKMGAEKVLIDGSLDRKSIVLSNSVDAIILT
                     AGASFGNQQAIIEELKRLITLSQIGTYLSPTLQKLAEQNKILIKNKGRWKQTGLMSLI
                     ANETKLLEILSNLEKPSHLYLPGAYTSSVNNRLGKHLSGIQLIFRHPECIKLSSNELE
                     VFLKNHKPLALIPFKLKAIALNPKGIGNSDIDADEFHQNLRRTFKEYPLLDVMEI"
     gene            complement(3599..4846)
                     /gene="ablA"
                     /locus_tag="PLW08_06705"
     CDS             complement(3599..4846)
                     /gene="ablA"
                     /locus_tag="PLW08_06705"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPL52326.1"
                     /translation="MSKLIDIKSIATPEQWNDWHWQIKNRITTHAQLSKYIELQPEEE
                     AVFKNKVFSFRMAITPHYLSLIDHSNPYDPIRLQAIPRIAESHISPSDMADPLSEDAD
                     APVPGMTHRYPDRVLLLLTDQCAMYCRHCTRRRKAGEHDAPMPKENVEKALEYIKEHK
                     EVRDVILSGGDPLTLSDERLDDILNRLSKIEHIEIVRLGTRTPVVLPQRFTDSLLKIL
                     QKYKFVWLNTHYNHPNELCEDSCKALAKIAETGIPMGNQSVLLKGVNDNVDVMKALVH
                     KLVKNRVRPYYIYQCDLSEGISHFRTPIAKGIEIMESLRGHTSGLCVPTYVVDAPGGG
                     GKIPVMPNYVISQMPGRVILRNYEGFITAYTEPEFVEQDKSKYRELCADEYKSTEGIM
                     ALMRGKKVAIGPAETKRNRRRKH"
     gene            complement(4861..5919)
                     /locus_tag="PLW08_06710"
     CDS             complement(4861..5919)
                     /locus_tag="PLW08_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPL52327.1"
                     /translation="MKSNGCRYGTHRVIEPKGVLPQPAKILNNDMSEIWDNEMLIDVI
                     RLNIDSASFHQIKNKLIAQGHQDLEKAFAEHAIELTNRTGKHKNEDTGSGGMFIGRVA
                     AIGDKFEMKEKVKVGDKIASLVSLSLTPLKINKVKKVLLDKDQMEIEGQAILFSSGVY
                     AKLPNDLDENLALSVLDVAGAPAQVERLVKPDDTVVIIGANGKSGILCNAVAKERAGI
                     GGKVIGVVRNESYIPTCKETGCDEVILASATDAITIQKEVSRLTNGKMADVVINVVNT
                     EDTELPSIMAAKDRGMVYFFSMATSFTKAALGAEGIGADVDMMIGNGYAHHHSEIALD
                     LLRRNPVLMKIFKERYAE"
     gene            6575..7876
                     /locus_tag="PLW08_06715"
     CDS             6575..7876
                     /locus_tag="PLW08_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL52328.1"
                     /translation="MIITFSVQNFLSIRNKVTLDFRATSDKTLEDYYVVSVEKPKLRI
                     LRMAMIYGANASGKTNLLMALDFIKLFISNTSINKEIPIPITPFALDEDKSSIFEIDF
                     LQNGVVYSYYLEMNTKQIIQEKLSHYDLGRKKLIFSRKLINIDDPKYKYIWKSADVDK
                     KQKDTLELTVHNQSILSRVATIEYSGPIQKARDWFTETLTRILDYKVDLFEYNYFNFL
                     NSHEKEQRYKSLYIELLKRADFCIEDFTIKERKITFTDVPPKLIAQIGAQHKEIGKKE
                     DVIYVLQTEFFHKTTSGSFSINYYNESMGTQRYFGLVGVLFELLYKNQVVPIDEIETS
                     LHIDLIIHFISTFLRNKSQGQLIFTSQNTALLKEKDILRRDAIWITERNEDGSTSLTS
                     VSEYPVRKEHSIESIYRKGLIGGLPNLGTTLLEGENETKEE"
     gene            7857..>8291
                     /locus_tag="PLW08_06720"
     CDS             7857..>8291
                     /locus_tag="PLW08_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HPL52329.1"
                     /translation="MKPRRNKPTKPFKNTVLCFCEGETEKVYLTCLKKDRYKNLHIEL
                     KPQISNADFEKTFKDIKHQLAKPKKTNYKYIFYVLDMDAIYNQNQIDKYNRQKESIEL
                     LVKAKERLAIIESRPCIEFWFLLHFKNTDKCFVNCDEIIVELC"
BASE COUNT         2521 a         1609 c         1469 g         2692 t
ORIGIN      
        1 tacagggatt tattgtgttt gctgttcttt tcggttttgg ttattatagt tacaatgttt
       61 tgatgtagca cgcagatttt atttttcaca aagaggaaaa gagaaaaaga gggtattttt
      121 tttcacaaag agaaaaagag gaaaagagat ttttttacaa tgagaacata gaaaaaactg
      181 agaacacctc tttctttgtt aatattgtct tttttcctct ttatctcttt gttcaaatta
      241 tctcttttcc tctttatctc tttgttagaa ttatctcttt ttctctttat ctctttgtta
      301 atattatctc ttttcctctt tatctctttg ttcaaattat ctctttatct ctttatctct
      361 ttgttaaaat tatctctttt tctctttatc tctttgttct atctattttt ttgcactggc
      421 gatcacaggt ttgggatgca tcaaattttc catatcctgc agaattatct gttgctggct
      481 tttgaacctt gccatctgcg tttcattcag cttttccgtt cctaccattt tcaagtttgt
      541 aggattgata tactttttac ctttgattaa accaaaatgc agatgagcac ctgtggaacg
      601 tcctgtagag ccaacgcgtc ctataatttg tcctttttta acggtctgtc ctttgcggac
      661 actcattgaa gaaagatgtg catattgagt tgtaagtcca ctgggatgtt ttatccgaat
      721 ttcatttccc cagccgccgt tataacctgc atccgtaact tttccattgg ctacggcata
      781 aacaggagtt ccataatgcg cacgataatc aactccctga tgattagccc agtttccata
      841 aaaaggatca aggcgtctgc caaaaggaga aacaagatga attacagata aaggaaagcc
      901 aattccgttg ctgtggcagc tttttccttc tttagtataa agaccgttta gcactgattt
      961 ttcttcctca tcttcataac ggaaaagttc acaagtgccg gttcgttcac cgctatattg
     1021 aacatagaat atttttctgc cgggcagggt tttcccttca aaaatgcgtt cttctacaaa
     1081 gacgcggaaa aaatcaccgt tacgcgcatc gcgggcaaaa tttatttctg tttccaagcc
     1141 gttatttatt gcttgtttat cctgaggagc aaaacccata gctaatagag aagcatccaa
     1201 ggtcccttct aattttcctt ccaggatgcg tttaactctt ttaacgggta atgcctccag
     1261 ggaatagaca agagaatcgg cactaaccgt aaaatgatga cgggtagttg gttcctgcac
     1321 aaacaccatt ttagcaatgt gttgtttatc ttccgaaagc aatattttca gcgtatcccc
     1381 aggttgaata gtggatacat caatatagtc accgaattta aaagccagca agccaacttc
     1441 tttcaagggt aaattcaaat ttgccagcac ggaaaaaata cttcctccct caggaatggt
     1501 ttgtattatc cagggttctg agggtttttg taaatcgggt ttaatttctg caggatgttt
     1561 aggattgata cccattaaac tgataagaca aaaagtagtt agaagcagaa aaataaaggc
     1621 gagaagaatg tcttttcccc aattcataag ttaactccaa tgtttgtttt agagcccggt
     1681 ttggggtctg tagaagagtg tttttcaaag taaggatgga agtgaatatg aacttgtctt
     1741 aagccggtgc tgaatttatc cagcaggcgt ttttctacag aatcggaaat aatattgcct
     1801 ttatcaacgc tgatatctcc atccactgta atagtcatat tcactgtaaa atagggaccg
     1861 aaacgatgaa tacccaggtc ttcaatacta cgaactcctt ccacagccat tgcttcggtt
     1921 ttcagttcac gcacaaaatc ctcgtcagga agataatcca tcagttctct tgaggattcc
     1981 attattattt ctactccggt ttttatgata taaatagcaa ctatagctcc ggcagctggg
     2041 tccatccaat aatagccaag acgacccaaa aggacaccaa ctacaacagc tacggctgcc
     2101 ataatatcat tcagatggtc attagccaat gccttgagag taggattttt ggttttgggt
     2161 atgtttttgc gagtgtaagt atagaggaaa attttcaagc caaaggtaaa gagcgctata
     2221 actaaagtcc aaatagaaag gggttgtcca atttccgttt tggaaagcaa atcatagacc
     2281 ttatttaccg attcccaaaa aatggctatt ccggtagtaa gaataaacgc gccaaccaca
     2341 attgcagaaa tagattccag ttgtctgtga ccataaggat gttctacatc ggcaggtttt
     2401 tgggcttgtt tcataaagat tttaacggca atgtaataga caacatcgga agtggaattt
     2461 ataccatcgg ctaaaagagc agcactatga cctaaaatgc ctacgcaggt tttgacaata
     2521 gataataaaa tattggaaaa cagacctaaa tttacagtta ctcgggttaa attatcgcgg
     2581 tccgtcacta ttttatatct ccatcacatc caacagagga tattctttaa aagttctgcg
     2641 taagttctga tgaaattcat cggcatcaat atcgctgtta ccaattccct taggattgag
     2701 cgctattgcc ttcagcttga aaggaattaa ggcaaggggt ttatgatttt ttaaaaagac
     2761 ctcaagttca ttactactaa gtttgataca ttccgggtga cgaaaaataa gttgaattcc
     2821 gcttaggtgt tttcctaaac gattattcac actgcttgta taggcaccgg gaagatagag
     2881 atggcttggt ttttccagat tacttaaaat ttccagcaac ttggtttcat tggcaattaa
     2941 agacatcaag cccgtttgtt tccatcttcc tttgtttttt atcaatatct tattttgttc
     3001 ggcaagtttt tgtaaggtgg ggcttaggta tgttccaatc tgagataaag ttatcagtcg
     3061 ttttagttct tcaattattg cctgctgatt accaaaactg gctccggcag ttaaaattat
     3121 ggcatcaacg gaattgctta aaacaatgga tttacggtcc agagagccat ctattagaac
     3181 tttttcagct cccatttttt gcagtaattc agcgcaggca atttgtccgc taacgcttgc
     3241 gggaccggtt atttccgttt ccagatattt ttcgcttttt actatccaga gttctctgct
     3301 tccgctttga aaagaggttt tagtcagaat gcttattccc gagccgtgtt gtaacagggt
     3361 tttagcatcg gtacaaaaaa aacagccctc aggaattttc actttgggct tgggaatttt
     3421 aaacaaagta tcttccgttt caccatcgcg tcctgtactc aaaatacccc acggaaaagg
     3481 atattttgcc agaatatagt tcaggacagt tgtcttacct gcatttttac agattcccac
     3541 aatcgctata tttttgtaac gcctgcagga aatccaggag aattccaaaa agtccccttt
     3601 aatgttttct acgacgatta cgcttcgttt ctgcgggacc tatggctact tttttacccc
     3661 gcattaaagc cattatacct tctgtggatt tatattcatc cgcacaaagt tcacggtatt
     3721 tgcttttgtc ctgttccaca aattccggtt ctgtgtatgc tgtaataaaa ccttcataat
     3781 ttcgtaaaat aactctaccc ggcatttggg aaataacata attgggcatc acaggaattt
     3841 ttcctccacc acctggagca tctaccacat aagttggaac acataatccg gaggtatgac
     3901 ctctcaaact ttccataatt tcaattcctt tggcaatagg ggtgcggaaa tggctgatac
     3961 cttcggataa atcacattga tatatatagt aagggcgaac tctatttttc accagtttat
     4021 gcaccagggc tttcattaca tctacattgt cgtttacacc tttcagcaac acggattgat
     4081 tgcccattgg aattcctgtt tcagcaattt tggcaagcgc tttacaggag tcctcacaaa
     4141 gttcattggg atgattataa tgcgtattca gccaaacaaa cttatatttc tgcaaaattt
     4201 tcaacaatga atccgtaaag cgttgtggca aaacaaccgg agttctggtg cccaaccgca
     4261 caatttctat atgttcaatc ttacttaatc tgtttaaaat atcatccaaa cgctcgtcac
     4321 ttaaagtgag aggatcgcct ccgcttaaaa taacatcgcg cacttctttg tgttctttta
     4381 tatattccaa tgccttttcc acattttctt tgggcatagg agcatcatgt tcacctgctt
     4441 ttctcctgcg ggtacaatgc ctgcaataca ttgcacattg atcagttaaa agaagcaaaa
     4501 cccgatcagg atagcgatgt gtcattccag gaacaggggc atcggcatct tcacttaaag
     4561 gatctgccat atcggatggc gaaatatggc tttccgcaat gcgcggaatt gcctgtaaac
     4621 ggatcggatc atagggatta ctgtgatcaa tcaaagaaag ataatgagga gtgatagcca
     4681 tccggaaaga aaatacctta ttcttaaaaa ccgcttcttc ttcgggttgc aattcaatgt
     4741 atttggatag ttgagcgtga gttgtgatgc gatttttaat ttgccagtgc cagtcattcc
     4801 attgttcagg agtggcaatg cttttaatgt caattagttt gctcattttg tcctctttat
     4861 ttattccgca tagcgttctt taaatatctt cattaaaaca ggattgcgcc gtaataaatc
     4921 taaagcaatt tcggaatgat gatgggcata accattacct atcatcatat ctacatcggc
     4981 accaattcct tctgccccca aagctgcctt tgtaaaagaa gttgccatag agaaaaaata
     5041 aaccatacca cggtctttgg ctgccataat actcggcaat tcagtatctt cagtatttac
     5101 aacattgatt accacatccg ccattttgcc attagtaagg cgcgagacct ctttttgaat
     5161 agtgatagca tccgtagcac tggcaagaat aacttcgtca caaccggttt ccttgcaggt
     5221 aggaatataa ctttcattgc gtactacacc gatcactttt ccaccaattc ctgccctttc
     5281 tttcgctacg gcattgcata aaataccgct tttaccgtta gctccaataa taacaactgt
     5341 atcatccggt tttaccagtc gctctacttg agctggagct ccggcaacat ccaaaacgga
     5401 aagcgccaaa ttttcatcca gatcattcgg caacttggca taaacgccac tgctgaaaag
     5461 aattgcttga ccttcaattt ccatttgatc cttgtctaac agcacttttt tcactttatt
     5521 tattttaagg ggagttagtg aaagcgaaac caaagaagca attttatccc ccactttcac
     5581 tttttccttc atctcaaatt tatcacctat agcggcaacc ctgccgataa acattccgcc
     5641 agaacccgta tcttcatttt tatgcttgcc ggttctgttg gtaagttcaa tagcgtgttc
     5701 cgcaaatgcc ttttccaaat cctgatgtcc ttgggcaata agcttgtttt taatctgatg
     5761 aaaggaagcg gaatcaatat ttaaacgaat aacatcaatc agcatttcat tatcccaaat
     5821 ttcgctcata tcgttattca atatttttgc cggttgcggt aaaacacctt ttggctcaat
     5881 tacccggtgc gttccatatc tgcatccatt acttttcatt ttcttctcct ctatgaatgt
     5941 ctattttatt atttaatttc aaattagcta taaaaatcca cctttttata tttgtccttg
     6001 ctgtcaattc ttttatataa gtaagctctt tctataattc tctccaaaac aggttatttg
     6061 ctttagtgaa ttcctttcct aaagcttttg actaacacga tatttgcttt ttttatattg
     6121 tcccttgctt gccaattttt tgctggctcg ctgccgatat aattccgata tgattccctt
     6181 atgattccca tatcacgata cgggaagcat aagggatctt attgaatagc atatcgttag
     6241 ccggcaaggg aagaacttat gataaaacct aaatgaaaaa gcaaaatgcc cgctctacaa
     6301 attttagctt gccaattaaa aaacccgcag gggagcacaa tgattgcgat ggtggtgtaa
     6361 atcacttgta aaatgataaa tataaaactt taaaacctta tctaaaactt tgggagagcc
     6421 ccgagggtga cacttctatt tagacggatt tttggctctg gggaaaattg tagtagggaa
     6481 attacagaaa aactgcaaaa tttcgcttga cattgaactg tatctaatta taatgtctct
     6541 aaaatgcaga agaaatgcaa ataaggagaa taaaatgatt attaccttta gtgtccaaaa
     6601 cttcctttcc atccggaata aagtaacttt ggattttcgt gccaccagtg acaaaaccct
     6661 ggaggactat tatgtagttt cggttgaaaa accaaagcta agaattctta gaatggctat
     6721 gatctatgga gctaatgcct ccggaaaaac taatctgctt atggctttgg attttatcaa
     6781 actatttatt agtaatactt ctataaacaa agaaattcct attccaataa ctccttttgc
     6841 tttggatgaa gataagtcat ctatttttga gattgatttt ctccagaatg gagttgtata
     6901 tagctattat ttggaaatga atacaaaaca aattattcag gagaagttga gtcattatga
     6961 tctgggaaga aaaaaactaa ttttttcgcg caagcttata aatatagatg atccaaaata
     7021 caaatacatt tggaagagtg ccgatgtaga taaaaaacaa aaagatactc tggaactaac
     7081 tgtccataac caatctattc tttcaagagt tgcaacgatt gaatattcag gtccgatcca
     7141 aaaagcaagg gattggttta ctgaaacttt aacacgaata ttggattata aggtagatct
     7201 ttttgagtac aattatttta atttcctcaa tagtcacgaa aaagaacaac gctataaatc
     7261 gctatatatt gaattgctaa aaagagccga tttttgtata gaagatttta ctattaagga
     7321 aaggaaaata acctttacgg atgtaccacc taaattaata gctcaaatag gtgctcaaca
     7381 taaagaaatt ggtaagaagg aagatgtaat atatgttctt cagaccgaat tttttcataa
     7441 aacaacatct ggctctttct ctatcaatta ttataatgaa tcaatgggaa ctcaaagata
     7501 ttttggtttg gtgggagttc tatttgaact attatataaa aatcaggttg ttccaataga
     7561 tgaaatagaa acttcattac atatagattt gattattcat tttattagca cttttttaag
     7621 aaataaaagc cagggacagt taatttttac ctctcaaaat acggctctct taaaagaaaa
     7681 agatatttta cgcagagatg ccatttggat tacagaaaga aatgaagatg gaagtacctc
     7741 acttaccagc gtttctgaat atcctgttcg gaaagaacat tctattgaaa gtatatatag
     7801 aaaaggatta atcggcggtt tacccaatct gggaactact ctcttggaag gtgaaaatga
     7861 aaccaaggag gaataaaccg acaaaaccat tcaaaaatac ggtattatgt ttctgtgaag
     7921 gtgaaacgga aaaggtatat ttaacttgtc ttaaaaaaga tagatacaag aatttgcaca
     7981 tagagctgaa accccagatc agcaatgccg attttgaaaa gacctttaag gatattaaac
     8041 accaacttgc taaacccaaa aaaacaaatt ataagtatat attttatgtt ttggatatgg
     8101 atgcgattta taaccaaaac caaattgata aatacaatcg tcaaaaagaa agtattgagt
     8161 tgctggttaa ggcaaaagaa agattggcaa taattgaatc cagaccttgt atagagtttt
     8221 ggttcctttt acactttaaa aatacggata aatgttttgt caactgtgat gaaattattg
     8281 ttgaattatg t
//
LOCUS       DAOOZV010000068         7767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8532_length_7767_cov_62.672459, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000068 DAOOZV010000000
VERSION     DAOOZV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8532_length_7767_cov_62.672459"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..549
                     /locus_tag="PLW08_06725"
     tRNA            459..549
                     /locus_tag="PLW08_06725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:494..496,aa:Ser,seq:gga)
     gene            711..2429
                     /gene="sppA"
                     /locus_tag="PLW08_06730"
     CDS             711..2429
                     /gene="sppA"
                     /locus_tag="PLW08_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPL52330.1"
                     /translation="MKSNKTVLWGCLIFVIALLAAFILGFIFTFGSHGAKSAKIPSNA
                     WLYVNPSALLPDYNELEELRFVNVSSPSVQEICAKIKLAAKDERIKGILLQPQMIMTN
                     YPALEEMSLAIGTFQKSGKPVLAFGDNFTQGDYLLASCADKIYMEPSASAGLVLQGVS
                     ANMLFYKEMLDKLGIKMHILQSGEYKGAGEPFSQTELSKGTKENIDAALYDIYNHLLS
                     LVAQNRKLETTYVKDIFEKRDDFFLSAEKAKELKLIDYAMSRDEMLKSLGLEEDNFVK
                     IANYSPTAKKKKGDKIAVVYLNGNIAPVSRSNFGSQGIISEAKVKKIIKQIHKHKDIK
                     AVVLRINSPGGSALESELIYQQLLKLKREYPLVVSMGGTAASGGYYISCAGDYLIADP
                     GTLTGSIGVIGLIPEMAGLGKKIGVRSQTLKYGKFAGALSPLEHYDPALIESLKRSST
                     ATYNEFKQRVMTARKISPENIEAVAEGRIFSAEDALANKLIDEIGTLDKAIAKAAGLA
                     KVTPYSSVNFPKKESYWEALRESDLLNLKERLQNNNDPATQLEKYLKQISATGEWLYL
                     MPYTLE"
     gene            2503..4020
                     /locus_tag="PLW08_06735"
     CDS             2503..4020
                     /locus_tag="PLW08_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPL52331.1"
                     /translation="MKKDSFNTEIIVNVHPLEKRVAVLEDSRLVELFVERREQQNIVG
                     NIYKGIVKDVLPGMGAAFIEIGLERTAFLHYSDIVMDFLDIYENALPRTKVNPEDSSQ
                     IDKLLKPGQEIVVQVHKGPIGSKGARLTGQISIPGKYLVLFPNKDKIAVSRKIYSQAE
                     RSRIRNLLAGIKDPGYGLIVRTEAEGCEEDDFQTEYKALIKTWHLIEKQIKYAKAPVC
                     VFEENALENYLIRDLFGENVDRLVIDDKNFAKRIISQLTDISPELINNIEIYREDSPI
                     FDAWGIEKKIETVLHSRIYLPSGGNIKIEQTEALVAIDVNTGSFTGKTNYDETVRKTN
                     LEAAAEIARQIRLRDLSGVIIIDFIDMVEEKYKNEVLEMLKKGLRRDRAKNKVYSFTE
                     LGLVEVTRKRMRNTLISNFSDPCPFCNGSGRILSKDSVIMRIYRWLNRSDYFIKDKNL
                     RIVVNPDLLNHIKQHNEDFTAYKEQIEFTSDPSIRIDQFKIYLLPGLEDITSKFS"
     gene            complement(4029..4331)
                     /locus_tag="PLW08_06740"
     CDS             complement(4029..4331)
                     /locus_tag="PLW08_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPL52332.1"
                     /translation="MLSRRNRVRIRGWGRKFSGRKRHGVCKAGQCLPLDQLPEGCNAV
                     VIHNNNLKIIERGLYIGAQISMFRNDDDEPNVVVAVGDARYVLDRRIAKTIKVKIV"
     gene            complement(4312..6492)
                     /gene="feoB"
                     /locus_tag="PLW08_06745"
     CDS             complement(4312..6492)
                     /gene="feoB"
                     /locus_tag="PLW08_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPL52333.1"
                     /translation="MRNRPVIALAGNPNVGKTCLFNALTGSHQTVGNWPGVTVEHKSG
                     RCNYKGKIYEVVDLPGIYSLAGGSPDELVARNYILTEKPDIIINIVDASNLERNLYLT
                     VQLMELGAPLIMCLSMIDIAEHNGIYIDTEHLSSHLGFAVFPLVLNKRIEVSPILDKV
                     EEYLVQPPVPATIHYDEVVEKHLQNIWQIVLKSGLGEPETKVPQRKFSESASIAYDIL
                     IKSRWQALKLIEGDAELTSRLSDEDRALVEKEISAIAKHRGQEPALVIADDRYGYIRG
                     LIKDVVKRKRKRQLTFSDMVDKVVLNSVVGLPVFFIVMYLVFLIAVKLSQPCINFIEF
                     ILSWLFIEQLGNLLSSLSFPGWLNYLLSKAIGGGIVTIGSFIPPIFFIYISLSILEDS
                     GYMARAAFIADKFMRKIGLPGKAFIPLLVGFGCTVPAIMATRTLEKPRDRVFASILTP
                     FVSCGAKLPVYTFLVMLFFPQYADLVIFGLYLFGIIMGVLTGLLLKKTIFLSAPGDFV
                     MELPAYHIPTINGIFMHTWHRLKSFLLRAGKTILLVIVLINILQGIQIPTGRDSEKTS
                     ILELGGKLITPVLQPMGIKRDNWQSSVALISGLFAKEAIVGTLQGLYQNSEGEISGDQ
                     LANNIKSSFGSNASILAYLVFILLYSPCAASLTMLFKEHGFKWMLFAFVYLTLIAWMV
                     ATLIYQILAFNVLSIFWFVVIGAIFTFIYITLRKIGSKYAFQTE"
     gene            complement(6482..6940)
                     /locus_tag="PLW08_06750"
     CDS             complement(6482..6940)
                     /locus_tag="PLW08_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPL52334.1"
                     /translation="MEEYAVIFNQYLSNKGLKLTKPRRCILETVFNMHTHFNVEELYD
                     KIRAKTNAISLATVYRTIPLLLDAGLVQHALRSEGRERYEHIFGHPKHIHWLCRNCGA
                     LMETDLNTLYPVLEKQAQDLKFKPEEIELNIKGLCWKCNPIENETQSDEK"
BASE COUNT         2532 a         1507 c         1474 g         2254 t
ORIGIN      
        1 agaacggaat ttcagctatc atccaacctg gcggttctaa aaacgatgag gaatgcattt
       61 ctgcttgcaa cgaactaaac atcgctatgg tctttaccgg agtccgtcat ttcaaacact
      121 gaaagtatca gtaacctgga ttttatgtta gcaagtatga aagataaaat ggcaaaaggg
      181 aataattatt ttgctttttc ggggatttat aggataaaat aattatctcg tgtttactta
      241 ccaggagttt taaagatagg aaagcaatgc ttctaactct gttgttttga atccaaaaaa
      301 ttagtttaac cttcaaattg tgtcctcact gctgaaacaa ttactgataa aaagagatta
      361 cctattaacc ctgatagagt ttttcaggga aaaggaaggc agatactaat tctatttgac
      421 agaaagaggt aagaaaaaaa gttgggaaaa ataagcaagg agatgtgtcc gagttggctg
      481 aaggagcacg attggaaatc gtgtaaacgt gtgagcgttt ctagggttcg aatccctaca
      541 tctccgccat ttttattgat tttttttaac ctgaaggata taaatactgc agcaggaaag
      601 caggttgcca aattccaatt tgccaaagct ctgaaagtta tttattatat cagtgttatg
      661 cggattatca gtagcggcat tcgttagtcg ctaaaaggag tttaaatatt atgaaatcaa
      721 acaagaccgt tctgtggggc tgtttaatct ttgttattgc cttattagct gcctttatcc
      781 tgggttttat attcactttt ggttctcacg gagccaaatc tgcaaaaatt ccctccaatg
      841 cttggttata tgtaaatcca agtgctttac ttccggacta taacgagctg gaagaattac
      901 gctttgtaaa tgtttcttct cccagtgttc aggaaatttg cgctaaaata aaacttgctg
      961 ccaaagatga gcgtattaaa ggaatacttc ttcagcccca aatgattatg accaattatc
     1021 ctgctttgga agaaatgtct ttagcaatag ggacttttca aaaaagcggc aagcccgtgc
     1081 ttgctttcgg agataatttt acgcaggggg actatttact ggcttcctgt gctgataaaa
     1141 tttatatgga accctctgct tcggcaggtt tagttttaca gggtgtttcc gctaatatgc
     1201 tattctacaa agagatgctg gataaattag gcatcaaaat gcacatttta caatccggtg
     1261 aatataaagg tgccggtgag cccttttcgc aaaccgagct tagtaaaggc acaaaagaaa
     1321 acatagatgc cgctctgtat gatatttata accatttact ttcccttgtt gcacaaaaca
     1381 gaaagttgga gacaacctat gtaaaagata tttttgaaaa gagagacgac ttctttttaa
     1441 gcgctgaaaa ggcaaaagaa ctgaaattga ttgattacgc tatgagccgt gacgaaatgc
     1501 tgaaaagttt ggggttggaa gaggataatt ttgtgaaaat tgccaattat tctccaaccg
     1561 ccaaaaagaa gaaaggcgac aaaattgctg tggtttatct gaatggaaat attgctcccg
     1621 tttcgcgttc caattttggc agtcaaggca ttattagtga agcaaaagtg aagaagatta
     1681 tcaaacagat tcacaaacat aaagacatta aagctgttgt tttaagaata aatagtcctg
     1741 gtggttccgc tttggaatcc gagcttattt atcagcaatt gcttaaatta aaaagagaat
     1801 atcctctggt tgtttcaatg gggggaacag cagcttcagg tggctattat atttcctgtg
     1861 ctggggatta tttaatagca gacccgggaa ctttaactgg ttcaattgga gtaataggtt
     1921 taattcctga aatggcaggg ttgggtaaaa aaatcggtgt gcgttctcag accttgaaat
     1981 atggcaaatt tgccggtgct ttaagtcctt tggaacacta tgatccagct ttaatagaat
     2041 ccctgaaacg cagttccaca gctacctaca acgaatttaa acaaagggtg atgactgctc
     2101 gcaaaatatc ccctgaaaat attgaagccg ttgctgaagg acgcattttc agtgctgaag
     2161 atgcccttgc caataaactt attgatgaaa tcggaacttt ggataaggca attgccaaag
     2221 ctgcaggtct ggcaaaagta actccatatt cctctgtaaa tttccctaaa aaggaaagtt
     2281 attgggaagc actaagggaa tcagacctct tgaatttgaa agaacggtta caaaataaca
     2341 atgatcctgc aacacaactg gaaaaatatc tgaaacagat atctgccaca ggtgaatggc
     2401 tatatttaat gccttataca ctggagtaaa ataaaaaaac agcaggatgg ttgatgtcct
     2461 catcaaccag acataactat ctaaaatata atttggacta aaatgaaaaa ggactcattt
     2521 aatacagaaa taattgtaaa tgtccacccc ttagaaaagc gggtagcggt tttggaagat
     2581 agccgcctgg tagaactctt tgtggaacgc agagaacaac aaaacatcgt gggcaacatc
     2641 tataaaggta ttgtaaaaga tgttttaccc ggaatgggag ctgcttttat tgaaatcgga
     2701 ctggaaagaa ccgctttttt acattatagt gacattgtta tggatttcct ggatatttat
     2761 gaaaatgccc taccgcgcac gaaagtaaat cctgaggact cctctcaaat agataaactc
     2821 ttaaaaccgg gtcaggaaat tgtggttcaa gtgcacaaag gacccattgg ttccaaaggt
     2881 gcacgtttaa caggtcaaat ctcaattcca ggaaaatacc tggtgctttt tcccaataaa
     2941 gataaaattg ccgtctcccg taagatttat tctcaggcag aacgcagtcg cattcgtaat
     3001 cttcttgccg gaattaaaga cccaggatac ggtttaattg ttagaaccga agcagagggc
     3061 tgtgaagagg atgattttca aacagagtat aaggcattga taaagacctg gcatttgatt
     3121 gaaaaacaaa ttaaatatgc caaagcacct gtttgcgtgt ttgaagaaaa tgctctggag
     3181 aactatctga ttcgtgatct ctttggggaa aatgtggacc gtttggttat tgacgacaag
     3241 aactttgcca aacgcattat ttcccaactg acagatattt cgcctgaact gataaataat
     3301 atagaaattt acagagagga ttctcctatt tttgatgcct ggggaattga aaagaaaatt
     3361 gaaaccgttc tgcatagccg tatttatctt cccagcggag gaaatattaa aattgaacaa
     3421 acagaagccc tggttgccat tgatgttaat accggaagtt tcactggaaa aaccaattat
     3481 gatgaaacag tgcgcaaaac caatctggaa gcagccgcag aaattgcccg ccaaattcgt
     3541 ttacgtgacc tttccggagt gattatcatt gattttattg atatggttga ggaaaaatat
     3601 aaaaacgaag tgctggaaat gctgaaaaaa ggtttacgcc gagaccgtgc caaaaataag
     3661 gtctattctt ttacagagct tggacttgtg gaagttaccc gtaaaagaat gcgcaatacc
     3721 ttaatctcca atttttctga tccctgtcct ttttgtaacg gaagcggacg cattttaagc
     3781 aaggactctg ttattatgcg tatttaccgg tggctgaacc gcagcgatta tttcattaaa
     3841 gataagaact tacgcattgt agttaatccc gatttgttaa accatatcaa acagcataac
     3901 gaggacttta ctgcatacaa agaacagatt gaattcactt ccgatccttc cattagaata
     3961 gatcagttta agatatatct gcttcccgga ctggaagata taacatccaa gttttcatag
     4021 tgcacttttc acacaatttt cacttttata gttttggcaa ttcttctatc caaaacatat
     4081 ctggcatcgc caacagccac aactacattc ggttcatcat catcgttgcg gaacatagaa
     4141 atttgcgctc caatatataa gcccctttca attattttca ggttattatt atggataact
     4201 acagcattac agccctccgg caattgatcc agagggagac attgacctgc tttacataca
     4261 ccgtgtcttt ttctgccaga aaattttcta ccccaacccc gaattcttac tctattccgt
     4321 ctggaaagca tatttactcc ctatttttcg caaagtaata taaatgaagg taaaaatagc
     4381 tcctataaca acaaaccaga agatggacag aacattaaaa gccagtattt ggtatatcag
     4441 ggtagccacc atccaagcaa tcaaagttaa ataaacaaat gcaaaaagca tccatttaaa
     4501 gccgtgttcc ttaaaaagca ttgtgagcga ggcagcacag ggtgaataga gcaagataaa
     4561 aacaagataa gctaaaatag aggcattact gccaaaactg cttttgatat tatttgccag
     4621 ctgatcgcct gaaatttctc cctctgaatt ttggtagaga ccctgcaagg ttccaacaat
     4681 tgcttctttg gcaaaaagtc cgctgattaa agctacggat gactgccagt tatctctttt
     4741 aattcccatc ggctgtaaaa caggagtaat caattttcct cctaactcca gaattgaggt
     4801 cttttcgcta tcccttcctg ttggaatttg aataccttgc agaatattta tcaatacaat
     4861 aaccagtaaa atggttttcc cggctctcag cagaaaactt ttcaagcgat gccaggtatg
     4921 cataaaaata ccgtttatag taggaatatg ataggcagga agttccatta caaaatcacc
     4981 cggagcactc agaaaaatgg tcttttttaa gagcaagccg gttaaaacac ccataattat
     5041 gccaaaaaga tacagaccga aaatcactaa atccgcatac tgagggaaaa ataacattac
     5101 cagaaaagta tacaccggaa gcttggctcc gcaagataca aaaggagtta aaatggaggc
     5161 aaaaaccctg tcccgaggtt tttccaaagt ccgcgtagcc ataattgccg gaactgtgca
     5221 accaaaacct actaataaag gtataaatgc cttacccgga agccctattt tccgcataaa
     5281 tttatccgct ataaaagctg ctcttgccat ataacccgaa tcttccaaaa tggataagct
     5341 tatgtatatg aaaaagatag gaggaataaa actgccaatg gttacaattc ctcccccgat
     5401 tgctttactt aataaataat tcagccatcc cgggaaggac aaagaactta ataaattgcc
     5461 caattgctct ataaacaacc agctcagaat aaattctata aaattgatgc agggctggct
     5521 gagttttact gcaattagga aaaccaaata cattacaatg aaaaagacag gcaatcccac
     5581 aacgctattc aacaccactt tatctaccat atcagaaaaa gttaactgcc ttttacgctt
     5641 gcgtttcact acatctttta ttaaaccgcg aatatagcca taacgatcat cagcaattac
     5701 caaggcaggt tcctgacctc tatgtttcgc tatggcagat atctctttct ctacaagtgc
     5761 cctatcttca tccgataaac gggatgttaa ttccgcatca ccctcaatca atttcaatgc
     5821 ctgccaacga ctttttatca aaatatcata ggcaattgaa gcagattcgg aaaatttacg
     5881 ctggggaact ttagtttccg gctctcctaa acctgatttc aaaacaatct gccaaatatt
     5941 ttgcaagtgc ttttccacta cttcatcata atgaatcgtt gccggaacag gtggttgaac
     6001 taaatactcc tccactttgt ccagaatagg agaaacctca attcgcttat tcaaaaccag
     6061 aggaaaaacc gcaaaaccaa gatgagaact taaatgttca gtatcaatat aaattccatt
     6121 gtgctctgct atatctatca ttgaaagaca cataataaga ggagctccca attccatcaa
     6181 ttgcactgtt aaatacaggt tgcgttccag gttggaagca tctacaatat tgatgataat
     6241 atcgggtttt tctgttaaaa tatagttgcg agctaccaat tcatcaggac ttcctcctgc
     6301 caaagaataa atgcctggaa gatcaacaac ttcatagatt ttgcctttat aattgcatct
     6361 tccggattta tgttcaaccg ttactccagg ccaattgcca actgtttgat gtgaaccggt
     6421 taacgcatta aaaaggcagg tcttacctac attgggattg cccgctaagg caattacagg
     6481 tctatttctc atcggattga gtttcattct caattggatt gcatttccag caaagccctt
     6541 taatatttaa ttcaatttct tccggcttaa attttaagtc ctgcgcctgt ttttccagaa
     6601 ccggataaag agtatttaaa tccgtttcca ttaaggcacc acaatttctg cacagccaat
     6661 gaatgtgttt cggatgacca aaaatatgtt catagcgttc tcttccttca ctgcgtaagg
     6721 catgttgaac aagccctgca tccaatagta agggaatagt ccgataaacg gttgccagag
     6781 aaatggcatt ggtcttagcc cgaattttgt catacaattc ctccacatta aaatgggtgt
     6841 gcatattgaa tactgtttcc agaatacaac gcctgggttt ggtaagtttg agtcccttat
     6901 tgctaaggta ttgattgaag ataacagcat attcttccat ctttttttaa cctctttgta
     6961 ttgataatca tttgcaactt aatttaactg acacattatg tcaagaaaaa aggttgagag
     7021 ggttgagaag gttgagaagg tttagaaggt tgagagagtt tagaaggttt agagggttga
     7081 gatgccctct gctcttagct cttagctctt tgccctttgc caaaaagccc ggagggcgaa
     7141 acaattatag cgatggtggc gtaagcccct cgaggaatga agccccgagt attttttact
     7201 tttttctttc ctttttaaaa aaggggctgt cccgaaacta aaaattagtt atgagacagt
     7261 taatataagc cattttaagg aacaatctcc tcttgaagat acattttggt atacctgaaa
     7321 atagcaaaaa atgaactttt gggaagttat gagacaaccc cgacacaatg atgtaaaagg
     7381 tggcgtaagc ccctcgtaaa attaatttac cttaaaatcc gcgaattaca caaaatctgc
     7441 gtaatctgcg tgaaacaatt tcataaagaa cattgtcctc cgtctttatt actttaaaaa
     7501 cggaagccaa caagaaattt gaaattgcta taaaatgtct cgtctatgtc actattatca
     7561 aatacgggag tgagacctct ttcatatctg aattcacaaa gaattttatt gaaatccaga
     7621 cccagattga gttggtagcc aataacgaag gaagataatt cagcaaattc ttcatctgta
     7681 taatcacctt cttccctaat agcattgctt aatcattgct tacccattgc taatgccatc
     7741 cgtaatgagt cagtaatgag taagcaa
//
LOCUS       DAOOZV010000069         7588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8769_length_7588_cov_49.486791, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000069 DAOOZV010000000
VERSION     DAOOZV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8769_length_7588_cov_49.486791"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1225)
                     /locus_tag="PLW08_06755"
     CDS             complement(29..1225)
                     /locus_tag="PLW08_06755"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPL52335.1"
                     /translation="MRQVVVVCAKRSPIGNFGGVFKDISAINLGVTVLQSMLKETGLK
                     PELIDEVIIGNVCGAGLGQNVARQIAIHSGIPETVPAYTVNKVCGSGMKAVSLATLMI
                     GSGEADIIVAGGTENMSQIPYALTDARWGARMGNKEMVDLMIRDGLSDIFNNYHMGIT
                     AENLAEKYHISREEQDAFAAQSQNRTEEAQKAGRFKDEIVPFSIPQRKGEPIIVDKDE
                     MPRAGVTVESIAKLRPAFKNDGTVTAANASGINDSSAFLILMSAEKAKELGLKPMATF
                     VNSASAGVDPSIMGYGPVPAIKKVLQKAGWTLQDIELAELNEAFAAQSLAVLKGLELE
                     GIGKLNPEIVNVNGGAIALGHPIGASGTRIIVTLLHEMKRRQVRKGLAALCIGGGMGI
                     ATLWEM"
     gene            1719..1868
                     /locus_tag="PLW08_06760"
     CDS             1719..1868
                     /locus_tag="PLW08_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52336.1"
                     /translation="MLIRYELKIETGHYTAKIDDINTYSSRKELTGNDYLHFKCKGNV
                     CRNEC"
     gene            1920..2414
                     /locus_tag="PLW08_06765"
     CDS             1920..2414
                     /locus_tag="PLW08_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52337.1"
                     /translation="MKKILLPLMLILITSFIFASTMQIGIGTNIQRYPLGSYYGYERS
                     AAIYKDSELGAQNIRITALSWYSTAAVTVNVPTKIYVKTTTETTLTKTTWANMISGAT
                     LVYDATHSSTLAGGWNQFDLSTFFDVPDGNGLIVMVERNYGGTGNGTSAGAGIRYTSV
                     NKYP"
     gene            2551..6606
                     /locus_tag="PLW08_06770"
     CDS             2551..6606
                     /locus_tag="PLW08_06770"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPL52338.1"
                     /translation="MELTQTLNWANGGGAPSGYKLSLGTVTPYTTILNQVNLGTATTY
                     DPELVYNTQYWWQVIPYNEGGDATNCPVWYFTTKLDPVISSFPYAEGFEVGNTHNTAI
                     YGWTQASVSGSNVWTANNSLTDYNRSPRTGSWNAFLRWSNSRWMFKPIQLQAGIAYRA
                     IVYARQDGATASDASIKICYGASASAAGMTNEILPSTGIVNGDYQRLEGTFTPSSTGL
                     YYLGILGTINNRPWYISIDDITIEEVPQIPVCSVNPEFWDFGELIMGLTSSKVFTITN
                     TGGATLYINNVYTTNSSYSILQQPGDCELSFNESTTFTVLFNPLEGGTQTGTVVICYN
                     GGEKTIALTGSCIDTTINSFPYTQSFENGGENWLVSAGVGATYYWELVRNDIAHGAVS
                     AYDGSYFARLNVYSAHYDYNPYCLISPPLNLSSGNKRLNYWVWIGANGYPAPLDVQIS
                     TDNQATWTTIFSHDLSVTNTWFNNIISLSSYSTSHIFIRFKATSNYGNGYCNLGLDKI
                     TIEDVPPTPIMSYTPGEIIFPYTNVKSVTDYINVTITNIGGGILSLSESSFTLQGSNP
                     NDFEINNFNLPANLGTGQSVNIPVRFNPVAEGNRTAILRIDDGLRSGYDVSLTGYAAG
                     QYVLCENFEGTFPAEDWTSPSTSWIRNSDFSHTGTASALAGYTAGTYWLVTPKLRPIE
                     GANTLTFWYRDYSSDTYWDYTNEYTYILVSKSTDFSSATTLWTGNYQTFTTSWQQASI
                     SLSAYNGKDIYIAFKHIATGGNYRMIDDITGVHLSFSTVPNPAVIVSPANEAEDVSLT
                     QTLNWTSGGGSPRGYYLSFGSVIPPLIYLDNVDMGTITSYTPNLDINKRYWWKVVPYN
                     EAGNAENCPIWTFTTMEDPTIRTFPYTEDFEGETFLPLGWTKIVHNGNDITKDSTKNH
                     SNPGQYSVRFSSYNFSDDYNQYLFTAPVFVRAPYTKLTFWHIKENASDETLEWGISTS
                     TNPADFTWNPVNLSSSEWQRTEINLRSYIGRKIYIGFHYYSNYKFYVYLDDIKIKATI
                     PANVPTPIADIVINSTIDLDLDESVDETNPIVQSLPNITALNNPVVLGLIGTETANMT
                     FEVGSGTWFGVCYYGGEWHHSIPYPCTVAEGATGTITFENVDFGAKGEVIVVMNEGQN
                     PTLPVELSAFTVNLNPQSGINIMWVTQSETGVNGYYIYRANVDTLSLASLISPLIKAS
                     NTSQQQVYLYTDKEIYESGIYYYWLETQDIDGVSIFYGSLSIDYKGNNNGTPEIPLVT
                     GIRSIYPNPFNPSTTISYELTKTAEVKIEIYNIRGQLVRSYALGKKERGRYKLLWEGD
                     DNNRRSCGTGMYFIRMQADKENFIKKVTLLK"
     gene            complement(6883..7206)
                     /gene="trxA"
                     /locus_tag="PLW08_06775"
     CDS             complement(6883..7206)
                     /gene="trxA"
                     /locus_tag="PLW08_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPL52339.1"
                     /translation="MAILEVNSANFETEVLKSTQPVLVDFWAPWCGPCRALSPTIEKI
                     ANETEGKVKVVKINVDEAQDIAAKYSVMSIPTLLIFVNGVVNDQLIGLVQKDKIMDKL
                     NKFIA"
     gene            complement(7211..7483)
                     /locus_tag="PLW08_06780"
     CDS             complement(7211..7483)
                     /locus_tag="PLW08_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPL52340.1"
                     /translation="MKIRPVEDHLAVKLSTTENEKTVGGIIIPDTAKEKPQIAEVIAV
                     GNDEDLQKIIKVGDKVLFGKYAGTEIELEGEKLLILAKSDLLAIVE"
BASE COUNT         2365 a         1458 c         1363 g         2402 t
ORIGIN      
        1 taatctgcgt aatctgcgtg ctatcattct acatttccca cagggtcgct attcccattc
       61 ctcctccaat acacaacgct gccaaccctt tccttacctg tcttcttttc atttcgtgca
      121 acaaagttac aataattctt gttccacttg ctccaatggg atgacccaaa gcaatagctc
      181 caccattcac attcacaatc tccggattca attttccgat tccttccagt tctaatcctt
      241 tcagcacagc taaggactgc gctgcaaatg cttcattcaa ttctgccaat tcaatatcct
      301 gcaaagtcca gcccgctttt tgtaacactt ttttaattgc tggcacagga ccgtaaccca
      361 ttatagaagg gtctacacct gctgaagcgc tattcacaaa agttgccatt ggcttcagac
      421 caagctcttt cgctttttct gcactcatca aaatcaaaaa agcagagctg tcatttatgc
      481 cactggcatt agctgcagtc accgttccgt catttttgaa ggcaggacgc aacttggcaa
      541 tggattccac tgttactccc gctcggggca tttcatcttt atctacaatt atcggctctc
      601 cctttctttg gggaatgctg aaaggcacaa tttcgtcctt aaatctaccc gctttttgtg
      661 cctcttcagt tctattctga ctttgggctg caaaagcatc ctgttcttct ctgctgatat
      721 ggtatttttc tgccaaattt tctgctgtga tacccatatg atagttattg aaaatatcgc
      781 taagcccatc ccgaatcatt aaatccacca tttccttatt acccattctg gctccccaac
      841 gcgcatcagt taaggcataa ggtatctgac tcatattttc cgttccacca gctacaataa
      901 tatctgcctc tcctgaacca atcattaaag tggctaaaga aaccgctttc attcccgaac
      961 cgcaaacctt atttacagta taggcaggaa cggtttccgg aataccactg tgaatagcta
     1021 tctgcctggc tacattctgc cctaaacccg ctccacaaac attaccgatt atcacttcat
     1081 caattagttc cggctttaat cccgtctcct tcaacattga ctgtaaaacg gttactccta
     1141 aattaatcgc ggaaatatct ttgaaaacac ctccgaaatt accgatagga ctacgcttgg
     1201 cacataccac tacgacctgt ctcattttgt ttctcctgtg ttgttaattt tcttattact
     1261 acattgcaaa aaaagtgccc cttttcgtca acaaaaatga tgtagcacgc agattacgct
     1321 gataacgcag attttatgat ttactaacat ataacatcct gcatatccag tcgatccagt
     1381 tatttgcgtt ctattaacct ttattcttcc ttcttcatta tttttccctt tcctctttct
     1441 ttgtgaaaat aaaaaaattt tagggggggt tgtggaaaaa tatcaacagt aatatgaggg
     1501 ttaacatctt tagattgaat atattagcaa ttatacctaa tctatatctc ataaatttaa
     1561 aaaaaaatac ccctgagagg gattgacata aatacgggga taaagaataa ggcaatggca
     1621 aatcaataaa tatgtttatt tgtttcataa ttgtttgatt tattatttta aatctttata
     1681 agccgttaaa tgatttaaaa tccttttaag gagatgttat gctaattagg tatgagttaa
     1741 agattgaaac cggacattat accgctaaaa tagatgatat aaacacctat tcttcacgca
     1801 aagaattaac tggaaacgat tatttacatt ttaagtgcaa aggaaatgta tgtagaaatg
     1861 aatgctaaag gacataactt agacaataaa aaaaaattta aaatcaggag taaacaagga
     1921 tgaagaaaat attacttcct ttaatgctaa ttcttattac atctttcatt tttgcaagca
     1981 ctatgcaaat tggaattgga acaaatattc aaagatatcc actcggttcc tattatggat
     2041 atgaaagaag cgcagccata tataaggatt ctgaattagg tgctcagaat ataagaataa
     2101 ccgctctttc ctggtattca acagccgcag taactgttaa tgttccaacc aaaatatatg
     2161 tgaagacaac cacagaaact actttaacaa aaactacctg ggcaaatatg attagtggtg
     2221 caacccttgt ttatgatgca actcatagtt ctactttagc aggtggttgg aaccagtttg
     2281 accttagcac tttttttgat gttcctgatg gaaatggttt gatagttatg gtagaacgca
     2341 attatggagg cactggaaat ggaacttctg cgggtgcagg aatacgctat acaagtgtaa
     2401 ataaataccc atgaatactg gcaagcagat aataatcctc ctactggaga tggaactcgt
     2461 aacaaacaaa gacctaatat tttaattcat tatatattac cgccacccaa tccggcgact
     2521 attgttagtc ctgccaatgg agcaattaat gtggaactta ctcaaactct caattgggca
     2581 aatgggggtg gagcaccttc aggttataaa ctctctttgg gaacagtaac tccttataca
     2641 acaatcttaa atcaggtaaa tttgggaaca gctacaactt atgacccgga attagtttat
     2701 aacactcaat actggtggca agtaattccc tataatgaag gtggggatgc aactaattgt
     2761 cctgtgtggt attttactac aaaactcgac ccggtaatca gctcttttcc ttatgcagag
     2821 ggctttgaag ttggaaatac acataacaca gctatttatg gttggactca ggcaagtgtg
     2881 agcggttcaa atgtatggac cgccaataat tccttaactg attataatcg ttctccccgt
     2941 accggttctt ggaatgcttt cttgcgttgg agcaatagcc gatggatgtt caaaccaatt
     3001 caattgcaag ctggaattgc ctatagagct atagtttatg ccagacaaga tggagcaact
     3061 gcttctgatg caagtatcaa aatatgttat ggtgcttcag cttctgcagc tgggatgact
     3121 aacgaaattt taccttcaac aggtatagta aatggtgatt atcagcgttt ggaaggcaca
     3181 tttactccct cttccacagg attatattat cttgggattt taggaactat aaataataga
     3241 ccttggtata tttctattga cgatataacc attgaagagg ttccccaaat tcctgtttgt
     3301 tcggtgaatc ctgaattttg ggattttgga gaactaatta tgggtttaac ctcctctaaa
     3361 gtatttacaa ttactaatac cggtggagct actttatata taaataatgt gtatactaca
     3421 aacagctcat attctatcct tcaacaacca ggcgattgtg aactttcttt caatgagtca
     3481 actactttca ctgttctctt taatcccttg gaaggaggaa ctcaaacagg aactgtggtt
     3541 atatgttata atggaggaga aaaaacaatt gctttaactg gaagctgtat agatactacc
     3601 atcaattctt ttccttacac tcagagtttt gaaaatggag gagaaaactg gttagtttca
     3661 gcaggagtgg gagcaacata ttattgggaa ttagttcgaa acgatatagc tcatggtgct
     3721 gtttctgctt atgacggttc ttactttgca cgcttaaatg tttattcagc tcattatgat
     3781 tataatcctt actgtttaat ttctcctcca ttgaatttga gttcaggtaa caagagattg
     3841 aattattggg tctggattgg agctaacggc tatccggctc ctttggatgt gcaaatttca
     3901 actgataatc aagcaacctg gactactata tttagtcacg atttgtcagt tactaacacc
     3961 tggttcaata atatcatttc tttatcaagc tattctacct cccatatttt tataaggttt
     4021 aaggcaacaa gcaattatgg aaatggatat tgtaatttgg gattagataa aataacaatt
     4081 gaagatgtac ctccaactcc tattatgagt tacacacccg gggaaattat ttttccttat
     4141 accaatgtaa aaagtgttac cgattatatc aatgttacaa taactaatat aggtggagga
     4201 atattaagct taagtgaatc cagttttacc ttgcagggca gtaatcccaa tgattttgag
     4261 ataaacaatt ttaatcttcc cgcaaatctc ggcacaggac aaagcgtaaa tattcccgtt
     4321 cgttttaatc cagttgcgga aggcaataga acagctattt taagaattga tgatggactt
     4381 cgatccggat atgatgtctc tctaactggt tatgcagcgg gacaatatgt cctttgtgaa
     4441 aattttgaag gaacttttcc tgcagaggac tggacttcac cttccacatc atggataaga
     4501 aattccgact tttctcatac cggaactgct tcagctttag ctggttacac tgccggaaca
     4561 tattggttgg taacacctaa attaagacca atagaaggag caaatacttt aactttttgg
     4621 tatcgggact attcttcaga tacatattgg gattatacta atgaatatac ttatatactt
     4681 gtttctaaaa gcacggattt ttcctctgcc acaactcttt ggactggtaa ttatcagacc
     4741 tttacaactt catggcaaca ggcatctata tccttaagtg cctataacgg caaagatatt
     4801 tatattgcct ttaagcatat tgcaacaggt ggaaattata gaatgataga tgatattacc
     4861 ggtgtgcatt tatctttttc tacagttccc aatccggcag taatagtaag tcctgctaat
     4921 gaagctgaag atgtttctct tacccaaaca ttgaattgga caagtggagg tggttcaccc
     4981 agaggttatt atctatcctt tggaagtgtt attcctccct taatttattt agataatgtg
     5041 gatatgggaa ctattaccag ctatacaccc aatcttgata taaacaaaag atattggtgg
     5101 aaggtagtcc cctacaatga agctggtaat gccgaaaatt gtccaatctg gacttttaca
     5161 actatggaag atcctactat tagaactttt ccttatactg aagattttga gggagagact
     5221 tttcttcctc ttggttggac taaaatagtt cataatggaa atgacattac taaggattca
     5281 acaaaaaatc attccaatcc cggtcaatat tcggttcggt tcagttccta caatttttcg
     5341 gacgactata atcaatattt attcactgca cctgtctttg ttagagctcc atatactaaa
     5401 cttacctttt ggcatataaa agaaaatgcc agtgatgaaa cattggaatg gggaatatct
     5461 actagtacca accctgcaga tttcacttgg aatccggtaa acttaagcag ttcagaatgg
     5521 caaagaacag aaataaattt aagaagttat ataggaagaa aaatttatat cggattccat
     5581 tactatagta attataaatt ttatgtttac cttgatgata tcaaaataaa agctactatt
     5641 cctgctaatg tccctactcc tatagcggat attgtgatca attctaccat agatttagac
     5701 cttgatgaaa gtgtggatga aacaaatcca atcgtgcaat ctttacccaa cattactgca
     5761 cttaataatc ctgttgtgct tggcttaata ggaacagaaa cagccaatat gacatttgaa
     5821 gtaggatcag gaacatggtt tggagtttgt tattatggag gagaatggca ccattcaatt
     5881 ccctatcctt gcactgttgc agaaggagct acagggacta taacttttga aaatgtagat
     5941 tttggagcaa agggcgaagt aattgtagtt atgaatgaag gtcaaaatcc tactttgcct
     6001 gtagaactat ccgcttttac ggtaaacctt aatcctcaaa gcggaattaa tattatgtgg
     6061 gttacccaat cggaaacagg tgtgaatgga tattatatct acagagctaa tgttgataca
     6121 ctttccctgg caagcttgat tagccctcta attaaagctt ctaatacttc tcaacagcag
     6181 gtatatcttt atacggacaa agaaatttat gaatcgggaa tttattatta ctggttggaa
     6241 acacaagata tagatggtgt ttccattttt tacggttcac tcagtattga ttataaagga
     6301 aataataacg gcactccgga aataccttta gtgacgggaa tccgctctat ttatcccaac
     6361 cctttcaatc cttccactac tattagctat gaacttacta aaactgcaga agtaaaaatt
     6421 gaaatatata atatccgcgg acaattagtc cgttcttatg cattaggtaa aaaagaaaga
     6481 ggtaggtata aactgctttg ggaaggtgat gataataata gacgctcctg tggaacagga
     6541 atgtatttca tcagaatgca agctgataag gaaaacttta taaagaaagt aactctcctg
     6601 aaatgaagaa tggcgagata gattttttac aaagagataa agagaaaaag agatttaact
     6661 gcgaattgag aatgaaatgt gaatagtgaa cggtgaaaga attaagtgag gagtcgtgac
     6721 ggagaaaaca atagaacgca gataactgga ttgattggat cagcaggatc ttatagagag
     6781 ggaattcaaa aaataaaaaa tctgtgttat cagtgaaatc tgtgagagat aagaaaaaca
     6841 gcccgggcaa taaatcccgg gctgtttgtt ttcagatata gcttaagcga taaacttatt
     6901 cagtttatcc ataattttgt ctttttgcac cagtcctatc aattggtcgt taacaacacc
     6961 gttcacaaaa atcagtaaag taggaataga catcacggaa tatttagcgg caatatcctg
     7021 tgcctcatct acatttattt ttactacctt tactttgcct tcggtttcgt tagctatttt
     7081 ttctattgta gggctaagtg ctctgcaggg accgcaccag ggagcccaaa aatcaaccaa
     7141 aacgggttgc gtggacttta gaacttcggt ctcaaagttc gcagaattaa cttccaggat
     7201 agccatttta tcactctacg atagccagga gatcgctttt ggcaaggata agcagctttt
     7261 ctccttcaag ttcaatttcg gttccggcat atttgccaaa gaggacttta tcgcccactt
     7321 ttattatttt ctgcaagtcc tcatcgttgc cgactgcgat tacttcagca atttgaggtt
     7381 tttctttggc agtatcggga ataattattc caccaacggt cttttcattt tctgtggtgc
     7441 taagtttcac cgctaaatga tcttctacgg gtctaatttt cattgtaacc tccataatgt
     7501 atttattttt caattataat ataagcgaaa tggcttactt agcaaacaca aaaatggact
     7561 gctaatttct gtcaagggaa aaatgaga
//
LOCUS       DAOOZV010000070         7585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8771_length_7585_cov_57.881275, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000070 DAOOZV010000000
VERSION     DAOOZV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8771_length_7585_cov_57.881275"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PLW08_06785"
     CDS             complement(<1..1696)
                     /locus_tag="PLW08_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52341.1"
                     /translation="MKFRKLEILPLGLILFFLCASISPCLAQFAGGTGSEAAPYLVAT
                     AQHLNNVRNYPGAFFLQTDDIYLNVEPYNSGLGWLPIGTQSAPFSGSYDGNNFHIYYL
                     YLNQSEGNNLGLFGYAHSAVLKNITLQDVYINGKDYVGSLIGSATECFLESCSSEGTI
                     TGASVTGGLVGSLELSCHLEKCFANCVVSGQFATGGLCGYSKGASEIYSSYSKGTVTG
                     NNATGGLVGIQGNSTLSQCFSTSAVQGNWGAGGLLGVNDTQSLVSYCYSLGSVTGNSN
                     TGGLIGRNVVSETLFSYWNVETSGQTISAGGNSRTTAEMTYPYAANTYVDWDLTEIWQ
                     PDTQYFVNGGYPYLQWMYPCPPISYLHYQIENYSVILQWSVPYVASYTGFNIYKNGVL
                     YATVTDSIFIDNNVYHREHYSYQVSAVYLGGESILSEPVSFTVYFPPLPANCVFPVNG
                     ATEMDIFLHLQWSPVAYNNYPLPDGYLLEVMSLAGFSVVNIDVGNVTSYQIPNPLDYE
                     TEYYWIVTPYTFGEGNTYLYAEDVPNWYFTTQEQIIKPINLTATIENWKNVQLNWNM"
     gene            complement(1839..3173)
                     /locus_tag="PLW08_06790"
     CDS             complement(1839..3173)
                     /locus_tag="PLW08_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52342.1"
                     /translation="MGQYLARCKFSFLFILTFCAGLNLNADWRMDFCFANLGSDGSLI
                     SATVYLGESAGAADSFDAFDFPLGPPPSNNPYVRAYFPHSDWGTTYNGNYMKDIRSIN
                     PAAKTWNLTFSKNGTPLSNNYRLSWEITGLFPACYQPRLIFNSQNINMLTQSSYSYTG
                     WINSGSIQMQYNNSMPYQLEPLPELLFSNNLTQELNLNRYFKVGSGTLSFSFLPNNNL
                     VQNMVTNQDSVWLNIYPVAGFTGTTFAEFSVIGTGGTLNAQISITRDATNSPPVFRDT
                     IQPLEIIQNQQGIFSWNGMVFDPDLDTVTINFSGGENISIEPNETLQQAIVIPALGFK
                     GETNFQFSLADNLHSPVIYAIPVTILPAVPASPQNLVLQLLPENSLLLTWQSVNTDTF
                     GLPISGLKYKVMLYADSSGTQLMHQYTNLTSSDILIPIESERMFIKVIAVNE"
     assembly_gap    3249..3348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3611..7495
                     /locus_tag="PLW08_06795"
     CDS             3611..7495
                     /locus_tag="PLW08_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL52343.1"
                     /translation="MERYSKLLPGMVEWTLQKRAEDNVSESFGCGYLFVDVCDFTKLT
                     ESASVKGHYGVEIITDILNQYFDLLNEKIMQYNGQIIKFEGDAVLAAFPGKEEICLCQ
                     MQECLKEFNRELQELNKRLKNKYGSDLSYHSSMGYGQSNVIILGKPNIHYDYFVYSPV
                     MPSLYNLFAQAGKNDCLQVKSGIKTKKKAEIYGAELPLRAEKSYDQSFFPPEILQRIS
                     SQTFTGELRNAAILFIGIAAEKYIHKGDYKTINNYYCKVQEIVYRLEGMINKIDYTDK
                     GLILLISFGILQTHIDDIERAIVCANLINSIESPLKAKIGLTYSNLYVGVLGAKQRCE
                     FGIIGTGVNVSARLMTSAKYGQIVFTKDILPSVQSRFEVQFLRKERVKGIKDELFFYC
                     ILRELPEFLNSYKRQYQNKTQICYQEQTAEIIQKIKDKKINQVLISGDHGTGKSFLSW
                     QILNNFYEENYKISIFVLDEFNHHDPLILHRKFISNELELNDPLSEPEKLQKYLAEIL
                     EERDADILLNALGLQNKETILTNDSGKQIEFLLLSLQKSLDLLMRDFDLILLDNIQWL
                     DDLSAQILQKRLEDDNPKSQTLILTTTKEITNYQNKANAKTEFISLRELNREEVIALI
                     RSQIPNITFQAVDYIYNLAGGNPRFITELCSQILSSFPDPDMLITESNIYDIQNKGLL
                     PYSVENLFIMKYESLSQEAKDILKKASIIGKGFTLNEIFETQSGISQNEIIPVISELQ
                     NNEIIDITTLSPEVQYLFNNALMRQAIYSTILLGEKVSLHNRIASFYEEKYGTLANRH
                     SELLAYHFHLGENKEKALHYALIAGDQNQKINNHSEAIYYYQIALQHTTERLEKIAII
                     LSIVDSQLYLSEVEVAKENLETIEPKEISHPELLKKYHFLRCRVYYLSGDYEGVLKYL
                     KNVTDFEGKYGEQIRVYQLDCLYRLFLIEDFSALLKDLKQEFVQQAANTLNVKSQKPS
                     ISTLLSRFQKIPEDEITEEQKHYLYLLLKLESIEANHFINTGYYQKALKSLLSQYELA
                     KALKDDLSLRIASNGLGVVYTRMGNLDSAYKAYVEAISIADKISDRFGFAKVLSDMAT
                     LHRRMGHHQEALDHFHRSLKIFESLGNLVFQGVVLHGIGEVYLQEGRDTEALKYFRKA
                     LKIARQSKDLFGISFEQDSIGDILFNSGKIADAKTQYLKNLKLQQKIGDNEGIAHTYG
                     NLGNVARMEKNLPLAIEYYTKNIKMTGEIGDKDGQGKGFYNLALVYEDLQDKEKAIQS
                     LHKALKCFTQAGSVKLIELTKQRLQENLQQNE"
BASE COUNT         2525 a         1410 c         1429 g         2121 t
ORIGIN      
        1 ccatattcca gttcaattgg acatttttcc agttttcaat agtggcggta aggtttatgg
       61 gtttaattat ctgttcttgg gtggtaaaat accaattggg cacatcttcg gcataaagat
      121 aggtatttcc ctcgccaaaa gtgtaaggag taactatcca ataatattct gtctcataat
      181 ctaaaggatt agggatttga taagatgtaa catttcctac atctatattc accacagaga
      241 aacctgctaa actcataact tccaataaat agccatctgg caaaggataa ttattataag
      301 caacaggaga ccattgcaaa tgaagaaaaa tatccatttc ggtagctcca ttaacaggaa
      361 aaacacaatt ggcaggcaga ggcggaaaat atacagtaaa agaaaccggt tcagaaagta
      421 ttgattctcc ccctaaatac acagcactaa cctgatagct ataatgctcc cggtgatata
      481 cattattatc tataaaaatg ctatcggtaa cagtagcata aagcactccg tttttataga
      541 tattaaagcc cgtataagaa gcaacataag gaacgctcca ctgtaaaata accgagtaat
      601 tttctatttg ataatgtaaa tatgaaattg gagggcaggg atacatccat tgcagataag
      661 gatagccccc attcacaaaa tattgagtat cgggttgcca aatttcggtt aaatcccaat
      721 caacataagt attagcggca taaggataag tcatttcagc agtagttctg ctatttcctc
      781 cagcagaaat tgtttgtcca ctggtttcca cattccaata gctaaaaagg gtttcgctga
      841 caacatttct gccaataagt ccaccagtat tactatttcc cgtaacgcta cccagagaat
      901 aacaataaga aaccaaactt tgggtatcat taactcccag taagccaccg gcaccccaat
      961 ttccctggac agcagaagta gaaaaacatt ggcttaaagt agaatttcct tgtataccaa
     1021 ccaaaccacc ggtagcatta ttacctgtaa cagttccttt gctgtaagaa gaataaattt
     1081 cactggcacc tttactatag ccgcaaagtc caccggtagc aaattgaccg ctgaccacac
     1141 aattagcaaa gcatttttcc agatggcagg atagttccaa agaaccaact aaaccacccg
     1201 ttacagaagc acctgtaatt gtgccttcgc tgctgcaact ttcaagaaaa cactctgttg
     1261 ctgagccaat cagggagcca acatagtcct taccgttgat gtatacatcc tgtagggtaa
     1321 tatttttgag gacagcagaa tgggcataac caaacaaacc caaattattt ccctccgatt
     1381 gattaaggta taggtagtaa atatgaaaat tattgccatc gtaagaaccg ctaaaaggag
     1441 cggactgagt gcctataggt aaccagccaa gccctgaatt ataaggttca acatttaaat
     1501 aaatatcgtc ggtttgtaaa aagaacgcgc caggataatt ccgcacattg tttaaatgtt
     1561 gtgcagttgc aaccaaatag ggagctgcct cagaacctgt tcctccggca aattgagcca
     1621 aacaagggct gatggatgca cagaggaaaa ataaaataag gcctaagggc aggatttcaa
     1681 gttttcggaa tttcatcgta tcctccagaa attatattag tttttggttt atagttaaaa
     1741 ttcatcgttt ttttgccggt tataatctcg cagattaagt tttttcatat tcatagaata
     1801 acttaagttt atctgtctgt ttttcatagt gttcacgctt attcgttaac tgcaataact
     1861 ttaataaaca ttctctcact ctctatagga ataagaatat cagaagaggt aagattagta
     1921 tattgatgca ttagttgagt tccggagcta tcagcataga gcataacttt gtatttaagc
     1981 ccgctaatag gcaaaccgaa agtatcagta ttaactgatt gccaggtaag cagaagagag
     2041 ttttccggta aaagttgcag aaccaaattt tgaggactgg caggaacagc aggcaaaatg
     2101 gttactggaa ttgcataaat aaccggggaa tgcaaattat ccgcaagaga aaattggaag
     2161 ttcgtttcac ccttaaaacc gagagccgga ataacaattg cctgttgcaa tgtttcattg
     2221 ggttcaatgc tgatattttc tccgccggaa aaattgattg taaccgtatc caaatcggga
     2281 tcaaaaacca ttccgttcca ggaaaaaata ccctgttgat tttggataat ttccaagggt
     2341 tgaatagtat ccctaaaaac gggcggtgaa ttagtagcat cacgagtaat agaaatttgg
     2401 gcgttaaggg ttccaccggt tccaataaca ctaaattctg caaaagttgt tcctgtaaaa
     2461 ccggctacag gataaatatt tagccacaca gaatcctgat tagtaaccat attctgcact
     2521 aaattgttat tgggaagaaa ggaaaaagag agagtgccac tgcctacttt aaaatatctg
     2581 ttcagattta actcttgggt aaggttattg gaaaaaagca attccggtaa gggttccaat
     2641 tgatagggca ttgaattatt atattgcatt tggatggaac cggagttaat ccaacctgtg
     2701 taagaatagc tactctgcgt aagcatattg atattttggc tgttaaaaat taaccggggc
     2761 tgataacaag cgggaaaaag tcctgttatc tcccaggaga gacgatagtt attacttaag
     2821 ggagtgccgt ttttagagaa agtaagattc caggtcttgg cagcaggatt gatagacctg
     2881 atgtctttca tataatttcc gttgtaagtt gtgccccaat cgctgtgagg aaaataggca
     2941 cgcacataag gattattact gggaggagga ccaagaggaa aatcaaaagc atcaaaacta
     3001 tccgcagcac ctgcgctttc tcctaaataa acagtagcac ttattaggga gccatcagaa
     3061 cccaaattgg caaaacaaaa atccattcgc caatcggcat ttaaatttaa ccctgcgcaa
     3121 aatgtaagaa taaaaagaaa agaaaactta caccgcgcta aatactgtcc catataatca
     3181 ccttcgtcag catttatcca taatttactt tactaatcct tcagcttttt ctgtcaagaa
     3241 atttctgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat aaacaggcaa
     3361 aacagtaagt atcctgctgt caggttgatt tatatttctg ctttgcccct tgcaaaaaaa
     3421 atttctttcc tcttttcctt tttatttaca agctatcatt tttggtgtat tcggtattcc
     3481 cggttttgtt tacaagtcat catcttcggt tttttcggta ttcagggtag ctaaaccaaa
     3541 ccctaatctt tttccttgac ctgtaaagtg gattttataa aagataaaaa ggtcttaaaa
     3601 gggagacaat atggaacgct acagtaaatt attacccgga atggtggaat ggacactgca
     3661 aaaaagagca gaagataatg tatcggaaag ttttggttgc ggttatcttt ttgtagatgt
     3721 ttgcgatttt acaaaattga cagaaagcgc ttccgtgaaa ggacattacg gagtagaaat
     3781 tattaccgat atcttaaacc agtattttga tctcttgaac gaaaaaataa tgcaatataa
     3841 tggacagatt atcaaatttg aaggtgatgc tgttttggct gcttttcccg gtaaagaaga
     3901 gatttgccta tgccagatgc aggaatgctt aaaggaattt aaccgggaac tgcaagaact
     3961 gaataaacgc ctgaaaaata aatatggcag tgatctttcc tatcatagca gtatgggtta
     4021 cggtcaatcc aatgtgatta ttctgggcaa accgaatatc cattatgatt attttgtgta
     4081 tagtccggta atgccttccc tttataacct ctttgcccaa gcgggaaaaa acgattgttt
     4141 gcaggtaaaa agtggcataa aaacgaaaaa gaaagccgaa atttatgggg ctgaacttcc
     4201 cctgcgagcc gaaaaaagct acgatcaaag tttctttcct ccggaaattt tacagcgcat
     4261 ttcttcccaa acatttaccg gtgaactgcg aaatgccgct attctcttta ttggtattgc
     4321 tgccgaaaaa tatattcata agggtgacta caaaaccatt aacaactact attgtaaggt
     4381 tcaggaaatt gtttaccgtc tggaaggaat gattaacaaa attgactata ccgataaggg
     4441 tttgatttta cttatcagtt tcggcatttt acaaacccat atagatgata ttgagcgcgc
     4501 tattgtctgt gccaacctta taaatagtat agaaagcccg ctaaaagcta aaatagggct
     4561 cacttatagc aatctctatg tgggagtttt aggagcaaaa caacgctgtg aatttggcat
     4621 tatcggcaca ggagttaatg tttcagcccg gttaatgact tccgctaaat acgggcaaat
     4681 tgtttttacc aaggatattc tacccagtgt gcaaagccgt tttgaagtcc aatttttgcg
     4741 taaggaaagg gttaaaggca tcaaagacga attgttcttt tattgtatcc tgcgtgaatt
     4801 gccggaattt ttgaacagct ataaaaggca gtatcaaaat aagacccaga tttgctacca
     4861 ggaacaaact gccgaaatca ttcaaaaaat aaaggacaaa aaaatcaatc aagtgcttat
     4921 ttccggagat catggcaccg gaaaatcctt tctcagctgg cagatattaa acaatttcta
     4981 cgaggaaaac tacaaaatta gcatcttcgt gttagatgaa ttcaaccatc acgatccttt
     5041 aatcctgcat cgtaaattta tcagcaatga actggaactg aatgatccct tgtcggaacc
     5101 cgaaaaactg caaaaatacc tggcagaaat tttggaagaa agagatgcgg atatcctttt
     5161 aaacgcttta ggactgcaaa ataaagaaac tatcttaacc aatgacagcg gaaaacaaat
     5221 tgaattcctc ttgctttccc tgcaaaaatc cctggacttg ctgatgcgtg attttgacct
     5281 tattttgctg gataatattc aatggctgga tgatctctcg gctcaaatcc tccagaaacg
     5341 cttggaagat gacaacccta aatcacaaac tcttatttta actactacta aagagataac
     5401 aaactaccaa aacaaggcaa atgccaaaac ggaatttatt agtttaaggg aattgaatcg
     5461 ggaagaagta atagctctga ttcgctctca aatccctaat atcacttttc aggcagtgga
     5521 ttatatctac aatttggcag gaggaaatcc gcggtttatt actgagcttt gttcccaaat
     5581 actttccagt ttccccgatc cagatatgct gattacggaa tctaatattt atgatattca
     5641 aaataagggt cttttaccct atagcgtaga aaatcttttt atcatgaaat atgagtcatt
     5701 aagtcaggaa gccaaggaca tcctaaaaaa ggcatccatt ataggtaagg gctttacctt
     5761 gaacgaaatt tttgaaaccc agagcggaat aagccaaaat gaaataatac ccgtaatttc
     5821 agaactgcaa aataatgaaa ttatagatat tacaaccctc tctccagaag ttcaatatct
     5881 tttcaataat gctcttatgc gccaagctat ctattctact attttgcttg gagaaaaggt
     5941 ttctctccat aatcgcattg cctcttttta tgaagaaaaa tatggaacac tggcaaaccg
     6001 ccacagtgaa ttacttgcct atcattttca tttgggagaa aataaggaaa aagctctgca
     6061 ttatgcttta atagcaggcg accaaaatca gaaaataaat aaccacagtg aggcaattta
     6121 ttactatcaa atagctcttc agcatactac agaaaggttg gaaaaaatag ccatcatcct
     6181 tagtattgtg gattcccagc tctatttaag cgaagtggaa gtggcaaaag aaaacctgga
     6241 aaccattgaa ccgaaagaaa tttctcatcc tgaactcctt aaaaagtatc attttttgcg
     6301 ctgcagggtc tattacttga gcggtgacta tgaaggtgtt ctgaaatatc tgaaaaatgt
     6361 aaccgacttt gaaggcaaat atggagaaca aatacgcgtt tatcaattgg actgccttta
     6421 tcggcttttc ctgattgaag atttttctgc cctgctaaag gacttgaaac aggaatttgt
     6481 tcagcaggct gcaaataccc tgaatgtaaa atcccaaaag ccatccattt caactttgct
     6541 ttctcgtttt cagaaaattc cggaagatga aattacggag gagcaaaaac actatctcta
     6601 tctattgctg aaactggaat ccatagaggc aaatcatttt atcaatacag gatattatca
     6661 aaaagcacta aaatccttac tttcccaata cgaacttgca aaagccctga aagatgacct
     6721 ctccttaagg attgccagta acggactggg agttgtttat acgcgaatgg gaaatttaga
     6781 ttctgcctat aaagcttatg tggaagcaat tagcattgcc gataaaataa gtgaccgttt
     6841 tggttttgcc aaagtccttt ccgatatggc aactttacat cggcgaatgg gacatcatca
     6901 ggaagcttta gatcattttc atcgcagctt gaaaattttt gaatccttag gaaatcttgt
     6961 ctttcaaggc gtcgtcttac acggaattgg agaagtgtat ttgcaggaag gacgcgatac
     7021 tgaagcattg aaatattttc gtaaagccct aaaaattgcc cgccaaagca aagacctctt
     7081 tggaatttct tttgaacagg attccattgg agatatttta tttaattccg gaaaaatagc
     7141 tgacgcaaag acacaatatt tgaaaaatct caaattacaa caaaaaattg gcgataatga
     7201 aggaattgcc catacttacg gtaatttagg caatgtagcc agaatggaaa aaaaccttcc
     7261 tctggcaatt gagtattaca ctaaaaatat taaaatgaca ggtgaaattg gcgataaaga
     7321 cggacaagga aaaggttttt acaatcttgc cttggtttat gaagacctgc aggacaagga
     7381 aaaagcaata caatccctgc ataaagctct aaaatgcttt acccaggcag gttcggttaa
     7441 actcattgaa ctgactaaac aacgcttaca ggaaaatttg cagcaaaacg agtgaaggat
     7501 gataaaaagg gctcacgcag atttcgcaga tgacgcagat taaaaatggc agagggctta
     7561 ggggttaggg cagagagaaa atagg
//
LOCUS       DAOOZV010000071         7547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8827_length_7547_cov_76.459423, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000071 DAOOZV010000000
VERSION     DAOOZV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8827_length_7547_cov_76.459423"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..2609
                     /locus_tag="PLW08_06800"
     CDS             93..2609
                     /locus_tag="PLW08_06800"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPL52344.1"
                     /translation="MKEDYIQKNDYRSIFFTDKAEECESELKSLFLNHLEYSLIKDIT
                     TVKPWDIYYALALSLRDLLIERWLRTQYEYRKQDVKKVYYLSLEFLLGRMLTNSLINL
                     DVYNEVYDMLKEMGISLEDIIELEPDMGLGNGGLGRLAACFMDSLATQAYPAYGYGIR
                     YDYGIFKQQIVQGYQVEEPDNWRKNGCPWEINRPELTYRVRFGGKVISETLPDGRIEH
                     HWIDTEDVLAVAWDIPVPGYQVDNVNNLRLWQATSTAEFDLEYFNNGDYVKAVEKKTI
                     SENISKVLYPNDNVHLGRMLRLQQEYFFVSATLQDIFAHWKRYHNSFNNFADKIAIQL
                     NDTHPALAIPEMLRILIDEERMSFENAWEITKRCFSYTNHTILPEALEKWGIALFEEL
                     LPRHLMLIYQINNVVMEEVVRLYPGDLAKMRNLSIIEETNGKAIRMAQLAIHSSHTVN
                     GVAKLHTKILCERIFPDLAELYPGKFQNKTNGITPRLWLHTCNPQLASLISEFIGSGW
                     ITNLEEIRGIEKYINDSDFRTSFAEVKDINKSHLSRYIYRETGIRVQVNSLFDAQIKR
                     LHEYKRQLLNVMGTIARYFRIKDNPNGNFVPRTVIFAGKAAPGYFLAKRLIKLINNIG
                     EVVNKDPDIKDRLKVVFLPNYCVSLAERIIPAADLSIQISTAGYEASGTGNMKFALNG
                     ALTLGTLDGANIEMAEEIGAENMFIFGLTAEEVKELNQSGYNPRSYYESDPELKRVVD
                     SLIDGTFEEGEKDLFLPIWKALMEDGDSYLNMADFRAFVETSNKVDEFYLNREEWITK
                     AILNVARVGKFSSDRAIKEYADDIWGIKPLPLELNNGNNS"
     gene            2615..4231
                     /locus_tag="PLW08_06805"
     CDS             2615..4231
                     /locus_tag="PLW08_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPL52345.1"
                     /translation="MAWIRNFILFAFWGFLCLALRAEIVINEVCYDPVGSDEGWEWIE
                     LYNNGNTSVQLEGAKILSGGSSFTLQYTFPFFELRPHRYLLIGGASILNAQLYYNFSF
                     QNGGSETDGIRYVSPDGTYTDTVLYDSPNTNQLFDDHNLAGTHFAPDVPAGYSLARIY
                     DGYDTNNCETDFIAEAKPTPGLPNRIHCDYALGTYSVLRENNYVELDLWLKNLSFLVP
                     ILSAELTITQNNELLAQQAIAPIPALDSLLVNLAFFCASSPLTVKLSLVDDPDSTNNV
                     LLVPLNPEMEEGIYINEFLANPESGNQEWIEIYGAQISQGTYYITDSSTSQIRFFLPA
                     TSGYYVICREPDALIWRYPECPAASIILAESWTYLNNAGDCLVLKNETGTLDSLNYSG
                     EEIIKGVSRERVTADSTVFWQNCYSEIGGTPGLPNSCMPQTELPDLGKVVLSGSPCNA
                     KAGEKISLAYNFPSATNRITCNIYDLNGRKICTVADYALVNAAGVLYWNGCKQDGNFA
                     PRGLYIVLWESQNAGGGKIMRKQFTAVLKG"
     gene            complement(4379..4879)
                     /locus_tag="PLW08_06810"
     CDS             complement(4379..4879)
                     /locus_tag="PLW08_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPL52346.1"
                     /translation="MIELREGDITLFEGEAIVNAANSSLLGGGGVDGAIHRAAGKGLL
                     EECRTLGGCKTGEAKITKGYNLKAQYVIHTVGPVWQGGNFREAELLASCYKKSLELAI
                     EKGIKSIAFPNISTGVYRFPKEEAGKIAVETVREFLPQHPEIDVYFYCFDRENYDIYC
                     RLLQEN"
     gene            5737..7344
                     /locus_tag="PLW08_06815"
     CDS             5737..7344
                     /locus_tag="PLW08_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPL52347.1"
                     /translation="MQAIRLKNIHFTYPNQYQEILSGITLDICDGQKIALIGKNGCGK
                     STLLKIIRGELKPTSGSISYPIAVPKIGYLPQDLMMSNQLSVRDFLLQGDINSYNIIR
                     EINILSAEKNLCVEDGLHLAELWQNYEHGNVLFWEQNVDEVIRKMDLNALANQSCNTL
                     SAGETTRVQLALLLLQNPDILILDEPTNHLDLQELMWLENWLNNYPKAILYVSHDRVF
                     IDNTASKIIELEKGKVVIRKGNYESFTKDKQELQEHQLKQYTQRTKLIGKLQEAAQKR
                     HNWAKSFQKETRPEGGGFVYESIFNAARTMNQQAKHIEERIIMLQNRFPIEKPIRDKT
                     RKIMFSPVQGQPKELLSLKDIDFSYGEKQVLHNLYFYLWGGEKIWLAGANGIGKTTLL
                     KIITGELKPTKGTVSYNNFLKFGVYNQNLNTLPGNLTILEYLKQFSDSEIEIRNFMGC
                     LGLTGDIVYQKMEVLSWGEKAKVQLTSLLCGKYNLLLLDEPTNHLDIKTREMLEQALN
                     QYQGAVIFVSHDRAFIQNLATRKFELK"
BASE COUNT         2332 a         1396 c         1534 g         2285 t
ORIGIN      
        1 tgccctaatg taaattcctc attttttact tgacaacaat tcccttttcc ccagttggaa
       61 aagtgttcat aagaacagag aaggtgcaat gaatgaaaga agattatata caaaagaatg
      121 actatcgctc catctttttt acggacaagg cagaagaatg tgagagcgaa ctgaagtcct
      181 tatttctgaa tcatctggaa tattcattaa taaaagatat aacaacagtt aagccctggg
      241 atatttatta tgcgttagct ctcagtttac gagacctgct gatagaaaga tggttgcgca
      301 ctcaatatga atatcgcaag caggatgtga agaaggtcta ttatctttcg ctggaatttt
      361 tgctggggag aatgcttacc aatagtttaa tcaatctgga tgtgtataac gaagtttacg
      421 atatgttgaa ggaaatgggt atttctttgg aggatattat tgaactggaa ccggatatgg
      481 gattggggaa tggtggttta ggacgtttag ctgcctgttt tatggattct ttagccaccc
      541 aggcatatcc tgcttatggg tatggtattc gttatgatta tggcattttc aagcagcaga
      601 ttgttcaggg ttatcaagtg gaagaacctg ataattggcg taaaaatggt tgtccctggg
      661 aaatcaatcg tccggaacta acttaccgtg tgcgttttgg gggcaaggtt atcagcgaaa
      721 cccttcccga tggcagaatt gaacaccatt ggattgatac ggaagatgtt ctggcagttg
      781 cctgggatat tcctgttcct ggttatcaag tggacaatgt gaataattta cgtttgtggc
      841 aggcaaccag cactgcggaa tttgatttgg aatatttcaa taatggcgat tatgtaaagg
      901 cagtggagaa gaaaaccatt tccgaaaaca tcagcaaagt tctctatccg aatgataatg
      961 ttcatttggg caggatgttg cgtttacagc aggaatattt ctttgtttct gccactttgc
     1021 aggatatttt tgcgcattgg aaacgctatc acaatagttt taataacttt gcggataaaa
     1081 ttgccattca gttgaatgat acgcatccag ctcttgctat tccggaaatg ttgcgaattt
     1141 tgattgatga agaacgaatg agttttgaaa atgcctggga aatcaccaag cgctgttttt
     1201 cttataccaa tcatactatt ttgcctgaag cgctggaaaa atggggtatt gctctttttg
     1261 aagaattact gccgcggcat ctgatgctta tttatcaaat aaataatgta gttatggaag
     1321 aagtggtgcg tttgtatcct ggcgatttag ccaaaatgcg taatctttcc attattgaag
     1381 aaaccaacgg aaaagctatc agaatggcgc aattggcaat tcatagttcg catactgtga
     1441 atggagttgc caaattacat accaaaatcc tctgtgaaag aatttttccc gatttggctg
     1501 aattataccc gggaaagttt caaaataaga ccaatggcat cacaccgcgt ctttggttgc
     1561 atacctgtaa tccgcaatta gcaagtttaa tttccgaatt tataggtagt ggttggatca
     1621 ccaatctgga ggaaattcgc ggtattgaaa aatatataaa tgactctgat tttcgcactt
     1681 cttttgccga agttaaagat ataaacaaaa gccatttaag cagatacatt tatcgcgaaa
     1741 caggaatacg cgtgcaggta aattcccttt ttgatgctca aataaaacgc ctgcatgaat
     1801 ataaacgcca attattgaat gtgatgggca caattgcccg ttattttcgc attaaagata
     1861 atcccaatgg taattttgtg cccagaacgg ttatttttgc cggaaaagca gcaccaggat
     1921 actttctggc aaagcgtttg attaaattga taaacaacat tggagaagtg gtaaataaag
     1981 acccggacat caaagaccgt ttaaaagtcg tctttttgcc taattattgc gtttctttag
     2041 ccgaaaggat tattcctgct gcagacctct ctatccaaat ttctactgcc ggttatgaag
     2101 ccagtggaac gggaaatatg aaatttgcct taaacggagc tttaacttta ggaactttgg
     2161 atggtgccaa tattgaaatg gctgaagaga ttggagcgga aaatatgttc atttttggct
     2221 tgactgcaga ggaagtgaaa gagcttaatc aaagtggtta taatcctcgc agttattatg
     2281 aaagcgaccc tgaactgaaa agagttgtgg attctttaat tgacggcact tttgaggaag
     2341 gtgagaaaga cctcttttta cctatctgga aagcattgat ggaagatggg gatagctatt
     2401 tgaatatggc tgatttccgc gctttcgtag agacctctaa taaagttgat gagttctatt
     2461 tgaaccggga agagtggata actaaggcaa ttctgaatgt agcacgtgtg ggaaaatttt
     2521 ccagcgacag agcgatcaaa gaatatgccg atgacatttg gggcattaaa cccctgcctt
     2581 tggaactgaa caacggaaat aattcctgat ttttatggct tggataagga actttatcct
     2641 ctttgctttt tggggatttt tgtgccttgc tttaagggca gaaattgtaa ttaacgaggt
     2701 ctgttacgat ccagttggca gtgatgaggg ctgggaatgg atagaacttt ataataacgg
     2761 taacacaagt gtgcagttgg aaggagccaa aatcctttcc ggaggcagtt cttttactct
     2821 tcaatacact tttccctttt ttgaattgcg tcctcatcgc tatttactaa ttggaggagc
     2881 gtcaatattg aatgctcaac tttactataa tttcagcttt caaaacgggg gaagtgaaac
     2941 ggatggcatt cgctatgtga gtcctgatgg aacttatacc gatacagttc tttatgattc
     3001 tcccaatacc aatcagttat ttgatgatca taatttggca ggaacgcatt ttgctcccga
     3061 tgttccggca ggttattctt tggcaagaat ttacgatggc tacgatacca ataattgtga
     3121 aacggatttt atagcggaag caaaaccaac tcccggcttg cccaatcgga ttcattgcga
     3181 ttatgcttta ggaacttata gtgttttgcg ggagaataat tatgttgaac ttgacctttg
     3241 gctgaaaaat ttaagctttc ttgttccgat tttatctgca gagttaacta ttactcaaaa
     3301 taatgagtta ctggctcaac aggcaattgc tccaattcca gctttggatt cacttctggt
     3361 aaatctggca tttttctgtg cttcttcgcc tttaaccgta aaattatcgc ttgtggatga
     3421 tcctgattcc accaataatg ttttgttagt tcccttaaat cctgaaatgg aagagggcat
     3481 ttatatcaac gagtttctgg ctaatccgga atccggaaat caggaatgga ttgaaattta
     3541 cggtgcccaa atttctcagg gaacttatta tattacagat agttccactt cccaaatccg
     3601 ttttttcctt cctgcaacaa gtggctatta tgtaatttgc agagaaccgg atgctttaat
     3661 ttggcgttat ccagagtgtc ctgccgcttc aataattttg gctgagagtt ggacttatct
     3721 gaataatgcg ggagattgtc tggttttgaa aaatgaaacc ggtactttgg attctttgaa
     3781 ttattccgga gaggaaataa taaaaggtgt ttcccgcgaa agagttactg cggattccac
     3841 tgtgttttgg caaaattgtt atagtgaaat aggtggaact ccgggacttc ccaacagctg
     3901 tatgccgcaa acagaactgc ccgatttggg aaaagttgtc ttatccggaa gcccttgtaa
     3961 cgctaaagcg ggagagaaaa tatctcttgc ctacaatttt ccctctgcca ccaatagaat
     4021 tacctgcaat atttacgatt tgaacggaag aaagatttgt actgtagcag attatgccct
     4081 ggtaaatgct gcgggagttc tttactggaa tggctgcaaa caggacggca attttgcccc
     4141 gcgcggtctc tatattgttt tgtgggaatc gcaaaatgct ggtggcggga aaattatgcg
     4201 taaacaattt accgctgttt taaagggata acgagcagga aaaagggttg agagggtaaa
     4261 gaaggtttag agagttgaga attccggaag gttgatatcc tcatcaagct ttcggaaaat
     4321 ttcctgccgt cataatgatg ttatgtaaac tatccctttt gtccttgctc tcctataatc
     4381 aattctcctg caataacctg caatatatat cgtaattttc ccgatcaaaa caataaaaat
     4441 aaacatctat ttcagggtgt tgaggcaaaa attcccgcac tgtttccaca gcaattttgc
     4501 ctgcttcttc tttgggaaaa cggtacacgc cggtagatat attgggaaag gcaatggact
     4561 taatcccttt ttctatagca agttccagcg attttttata acaggatgcc aataattccg
     4621 cttccctaaa gttacctccc tgccaaacag gaccaactgt atggataaca tattgtgcct
     4681 taaggttata acccttggta atttttgcct cgccggtttt gcatccacct aatgttctac
     4741 attcttctaa aagacctttt ccggcagctc tatggattgc accgtcaact cctccgccac
     4801 ctaaaaggga gctatttgcc gcatttacaa ttgcttcgcc ctcaaaaagg gttatatcgc
     4861 cctctctcag ttctatcatt ttttcacctc ctttttgcga ttgggatgta aaattccgtt
     4921 ttgccacaaa gaacatagtc cttgatggct ataccttcaa actttgccga agcgaaattc
     4981 taatgctgaa aaccgctaac aaaagtgcct aatttcgtca atggaaataa gatatatacc
     5041 ttatccggca aacaaaaacc gcaatccgat agttcttttt tgctcattaa aaagaccctc
     5101 caatttaagc gtttaactcc ttatttatta gttggttctt aatcaattcc gtaacactac
     5161 cgtaacacta ccgtaacact tctgtaacac tgcagtaata tcgttacttg atcgttatag
     5221 atttgttaca aatatattac cggacgcagt aaggaactga aacgacaacc aaggcagtgt
     5281 tattattcct tgactttttt ccaatgcata taaaaagaga acaatataat aaacaaagga
     5341 gattgaacca atgatcaact gcaaaattta aggcataaca gtcccggaaa atgctcttat
     5401 ctcattgtta aaaagaaaat tgctatttcc tttttgcact atcgggaagc ccaatagttg
     5461 ttcttatttt ccaagtggca tttgcatatt ttgccattct tgagatagca ggaatcccta
     5521 caattaaaat ggattccgcc acaaatgcga aatgacaagc ggtcttttta ctgccattct
     5581 tgcgtatctt tgtccttcct gcaaaagcgg gaatttagat aagggtaact tcttctggca
     5641 tataacttta aagaactatc tcaccctttc tctcataaag aaagctgtta tgcctgcccg
     5701 ccaagaaaac tgctaaaaac aattaaagga gaaaaaatgc aggcaatacg gctaaaaaat
     5761 atacacttca cctatcccaa tcaatatcag gaaattcttt ccgggattac tctggatatt
     5821 tgtgacggac aaaaaatagc tctcatcggg aaaaacggtt gcggaaaatc tactttgcta
     5881 aaaattatta ggggggaact gaagcccact tcgggttcta tttcctatcc tatagctgtt
     5941 cccaaaatcg gttatctgcc tcaagacctg atgatgagca accaattaag tgtgcgtgat
     6001 tttttattgc agggagatat taatagctat aatattatta gagaaatcaa tatcctctct
     6061 gcagaaaaaa acctgtgcgt tgaagatggc ttgcatcttg ctgaattatg gcaaaattat
     6121 gagcacggca atgttttatt ctgggaacaa aatgtggatg aagtaattag gaaaatggac
     6181 ttgaatgccc tggcaaatca aagctgtaac actctttccg cgggagaaac aactcgcgtt
     6241 cagcttgcct tgcttttatt gcaaaatccg gatattttaa ttttggatga accaacaaat
     6301 catcttgacc tacaagaact tatgtggctg gaaaattggc tgaataatta ccccaaagct
     6361 atcctttatg tttcccacga tagagtgttt attgataata ctgcctccaa aattattgaa
     6421 ctggaaaaag gaaaagttgt tatccgaaaa ggcaattatg aaagctttac caaagacaaa
     6481 caggaattgc aggaacacca gttaaaacaa tatacgcaac gcacaaaact gattggaaaa
     6541 ttgcaggaag ctgctcaaaa aagacataat tgggcaaaaa gctttcaaaa agaaacccgt
     6601 cccgaaggtg gtggatttgt ttatgaatca atttttaacg ctgccagaac gatgaatcaa
     6661 caggcaaaac atattgaaga acgtataatt atgctgcaaa accgttttcc gattgaaaaa
     6721 ccgataagag ataaaacccg taaaataatg ttttcccctg tccaagggca acccaaagaa
     6781 ctgctttcct tgaaggatat tgacttttct tatggcgaaa aacaggtctt gcataacctc
     6841 tatttttatc tttggggagg cgaaaaaatc tggcttgcag gagctaacgg aatagggaaa
     6901 actactctgc tgaaaataat taccggcgaa ctgaaaccaa caaaaggaac cgtttcctat
     6961 aacaatttct taaaatttgg tgtctataat caaaacttaa acacccttcc agggaattta
     7021 actattttag agtatctgaa acagttctct gatagcgaaa tagaaattcg taattttatg
     7081 ggatgcttag ggttaaccgg agatattgtc tatcagaaaa tggaagttct ttcctggggt
     7141 gaaaaagcaa aagtgcaact aacttctttg ctttgtggaa aatataatct tctccttttg
     7201 gatgagccaa ccaaccatct ggatattaaa acccgggaaa tgctggaaca agcattaaat
     7261 caatatcagg gtgcagttat ttttgtctcg cacgataggg cttttattca gaatttggca
     7321 acccgcaagt ttgaactgaa ataaagcaaa aggtaaagag ccaagagaaa aagggttaag
     7381 gaaatatttt cactcacaat tttgcaggtt tcagcccaaa aacaattctc ctcaatttta
     7441 tatctatcca actttatctt atctaactaa gctataagtg aattccgtgg ttcaaaatta
     7501 gaaggaaaaa aaattgaaga tgaatccaaa tataagattg acagaat
//
LOCUS       DAOOZV010000072         7478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8920_length_7478_cov_52.560555, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000072 DAOOZV010000000
VERSION     DAOOZV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_7478_cov_52.560555"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..744
                     /locus_tag="PLW08_06820"
     CDS             97..744
                     /locus_tag="PLW08_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPL52348.1"
                     /translation="MLTYTEIRYNAREYLKGKWNNPCALIILILYLINGGLSLIPYLG
                     SVVSFLILGPFSLGLAIVFLKLVRGEEVNVELMFAGFKDFTRSLTAGLLIFIYVFLWT
                     LLLIIPGIIASYAYAMTFFIMADNPNISANEAIKASKEMMKGHKTDLFLLDLSFIGWI
                     LLSVLSFGIGFLWLGSYIYTANAIFYHEIRVEEASQVIIEAPYEEQTVPPPDIEE"
     gene            complement(754..2145)
                     /locus_tag="PLW08_06825"
     CDS             complement(754..2145)
                     /locus_tag="PLW08_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL52349.1"
                     /translation="MRNIDKKQKHSRLRISFQTSITEGIFAQIYGNLSAIGSSFIVKL
                     MVILGASPMHYSLLSAIGQVSALWQPLGVAITHKLKERKSACIKITAIGRFLTIFLGL
                     SLLFNNHLQGIWFVLILLFFSAGLQSLGANIWIAWISDLIPLSIRGRFFSRRNQILIG
                     IGLIVSYIVSFHIDLFESAKGAFKLGYIKLLRAEKFFLPQNQPWFLAGVFVFASLIGL
                     IGLFFLNRQPERPLRPVPEQRLRERYSAPFKDKNFRLLSVFGIWWMLAIGVGSPFWGP
                     FMMKKLSMSLFEIQIYNTLSVFSSLLSYQFWGRFIDKNGNKTAMQICIALGGLNPMLW
                     LFTSAGNYSILWLEALSSGFMWAGAGIITTNFILSVAEKGKEQVYSGIYGAITGLAMM
                     FSTLACGIFYPSSLTIGKRILEPEQVIFGVGGIMRWLSFIPLFFVVEKRTVPLPKALL
                     SLFREKTDRSLKK"
     gene            complement(2479..3750)
                     /gene="eno"
                     /locus_tag="PLW08_06830"
     CDS             complement(2479..3750)
                     /gene="eno"
                     /locus_tag="PLW08_06830"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPL52350.1"
                     /translation="MSDILYIIGREILDSRGNPTVEAEVHLDSEIMAIAAVPSGASTG
                     EREAIELRDGDKKRYGGKGVLKAVSNIDNISDELWGTDSLLQGQIDKIMIDMDGTPNK
                     AKLGANAILAVSMAVARASALELNIPLYRYLGGVNAKTLPVPMSNILNGGAHADNNVD
                     IQEFMIMPLGAKSFKQALQMNAEVFHSLKAILKKRGLVTSVGDEGGFAPNLASNEEAF
                     IVIVEAIKTAGYIPGKDIYLAIDAAASSFYKDGKYLFEGKKVSSDAMIKYYESMIAKY
                     PICSIEDGLAENDWTGWKKMTAKLGSKIQIVGDDVFVTNPKIIAKGIKDNIANSVLIK
                     LNQIGTVTETIDAINMAHKAGWTTVVSHRSGETGDTFIADLAVALNTGQIKTGSISRS
                     ERIEKYNQLLRIEEELGEAAYFPGKSIIKQL"
     gene            complement(4190..5284)
                     /locus_tag="PLW08_06835"
     CDS             complement(4190..5284)
                     /locus_tag="PLW08_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HPL52351.1"
                     /translation="MKPFKPEKLPLSCIDWERQISFISQAHNLLGKYEGMLQTIVNPN
                     LFLSPLTTQEAVLSSKIEGTQATLDEVLRYEANPKMTLSPERNADIQEILNYREAINY
                     AVERLKSIPLSLNLIKDVHSILMNSVRGMNKTPGEFRKVQNYIGPPGADISQATYIPP
                     SPEEVMPAMDNLEKYIHYEEKDTIVQLAIIKAQFEIIHPFLDGNGRIGRILIPLFLFY
                     KNIISKPVFYLSTYLEKHRDEYYQHLNNITAKNDWNGWISFFLKAVIEQAKMNIEKAG
                     AIQNLYDQMKITIPEITGSKYSMQLTDALFISPLFSINGLSTASMIPKATIIRIVNIL
                     KNEKIVTEIQKSRGQMPGLFKFKALYNIVK"
     gene            complement(5611..7239)
                     /gene="groL"
                     /locus_tag="PLW08_06840"
     CDS             complement(5611..7239)
                     /gene="groL"
                     /locus_tag="PLW08_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425343.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPL52352.1"
                     /translation="MAKQMQYAHDARTSLKSGVDKLADAVKVTLGPKGRYVVLDRKFG
                     SPTITNDGVTIAKEIELENEYENMGAQLCKEVAEKTHDTAGDGTTTATILAQAIIEEG
                     LKHVTAGVNPMYLKRGLEKATKVVVDKIREFSKEIKSSEEIAQIAAISANNDPEIGAL
                     IAEAMDSVGREGIINIEEAKSIDTGLEKVEGMQFDRGYISPYFVTNAEKMIAELEEAF
                     ILLYDKKITALKELLPILQEVSQNGRPLLIIAEDIEGEALATLVVNKLRGVLNVVAVK
                     APGFGDRRKAMLEDIAVLTGATLISEEMGRKLDSATMSDLGRAKKIIVEKENTTIREG
                     AGNPEEIEGRIKQIRAQIEETTSDYDKEKLQERLAKLSSGVAVLRIGAATETEMKEKK
                     ARVDDALHATRAAVEEGIVPGGGITLIQAAKALKQLKGLSYEEKMAVDILSKALEKPA
                     YQIAANAGEEGAVVVEKLKSFRDIHMGFNASNGQFEDLFKAGIIDPAKVVRSAIQNAA
                     SIAGLMLTTECIITDIKEPEPAVPMPNPNMADMY"
BASE COUNT         2206 a         1497 c         1286 g         2489 t
ORIGIN      
        1 tgagcagcta cattttttgt catactccca aaaaataact tgacttaagt tgcccctaca
       61 aaataggata aaaaaaagaa atcaagtgag gtgattatgc taacctatac tgaaattcgt
      121 tataatgcca gagagtattt aaaagggaaa tggaacaatc cctgtgcgtt gataattctt
      181 attttatacc tgattaatgg tggattatct ttaattcctt atcttggttc ggttgtttct
      241 ttccttattt taggaccttt ttctttggga ttagccatag tttttcttaa attagttaga
      301 ggtgaagaag ttaatgtaga attgatgttt gccggattca aggattttac ccgtagttta
      361 acggcaggat tattaatctt catttatgtg tttttatgga ctttgctttt aattattcct
      421 ggaatcattg cttcgtatgc ctatgcaatg acttttttca taatggcgga taatcccaat
      481 atttcagcta atgaagcaat taaagccagt aaagaaatga tgaaaggtca caaaacagac
      541 ctttttttgc tggatctttc ttttattggt tggatattac ttagtgtctt atcgttcggc
      601 ataggtttcc tgtggttagg cagttacatt tacactgcca atgcaatttt ctatcatgaa
      661 atcagagtgg aagaagcatc tcaggttata attgaagccc cttatgaaga acaaactgtt
      721 cctcctcctg acatagaaga ataaatcgtt caattatttc ttaagcgatc tatccgtttt
      781 ttcccgaaaa agggaaagca atgccttagg caaaggtaca gtcctttttt ccaccacaaa
      841 aaacaaagga ataaaactta gccaacgcat aattccacct acaccgaaaa tcacctgttc
      901 cggttctaaa atccttttac caattgtgag tgaggaagga taaaaaatac cacaggcaag
      961 agttgaaaac atcattgcca agccggttat ggctccgtaa attcccgaat aaacctgttc
     1021 tttccctttt tcagctacgg aaagaataaa attggtagtt ataattcctg ctccagccca
     1081 cataaaaccc gaacttagtg cttccagcca caaaatggaa tagtttccgg cactggtaaa
     1141 aagccacaac ataggattta atcctcccaa ggcaatacaa atctgcatag ctgttttatt
     1201 gccattttta tctataaagc gaccccagaa ttgataagat aaaagtgagg aaaacacact
     1261 caaagtatta tagatttgga tttcaaacaa gctcatagaa agtttcttca tcataaaagg
     1321 tccccaaaaa ggactgccta caccaatagc taacatccac caaataccaa aaacggaaag
     1381 cagacggaag tttttatctt taaaaggagc tgaataacgc tcccgtaaac gctgttccgg
     1441 aacaggacgc aagggtcttt caggttgacg gttcagaaaa aataaaccaa ttaaaccaat
     1501 aagagaagca aacacaaaaa ccccggctaa aaaccagggc tgattttgag gaagaaagaa
     1561 tttctctgct ctcaaaagct ttatgtagcc aagtttgaaa gcacctttgg cggattcaaa
     1621 aaggtctata tgaaaactga ctatgtaact tactatcaat cctattccaa tcaaaatttg
     1681 attgcgcctg gagaaaaagc gtccccgaat ggaaagagga atcaaatcac ttatccaggc
     1741 aatccagata tttgctccca aggattgtaa acctgcacta aaaaatagca gtataagcac
     1801 aaaccagatt ccctgtaagt gattattaaa aagcaaagac aaccctaaaa agatagtcaa
     1861 aaacctgcct atagcagtaa ttttaataca ggcagattta cgttccttca gtttatgagt
     1921 aatagctact cccaaaggtt gccaaagagc agaaacctgt cctatagcgc ttaataaact
     1981 gtagtgcatt ggtgaagcac ccaaaattac catcagcttt acaataaagg aagaaccgat
     2041 tgcagacagg ttaccgtaaa tctgagcaaa aataccttct gttatagagg tctgaaaact
     2101 aattctaaga cgactatgtt tctgcttttt atctatgttg cgcatctttt ttatactcat
     2161 attattcatt gtagggaagt ggttgttctt gtccagagaa tttacactac aaggagtatt
     2221 aaatagtaaa aagtgaaatt aggtatttgg tttgctgaat tcagattcgc cataaattga
     2281 gagcatttat ttttcagagt tttgcgcact ctttggcgaa gaatgaattt gccaacccat
     2341 ttttcctaaa ctgcattgtt ttcggataaa ataaatgtct ttacaatctg aaaactatac
     2401 ccaatggtaa aataataact cttttaatcc caaaaccgga gctgtcaatt ataacagctc
     2461 cggcaaggtt tttttatctt atagttgttt tattattgac tttcctggga aataagctgc
     2521 ctcacctaat tcttcttcaa tgcgtaggag ttgattatat ttttcaatac gttcgctgcg
     2581 ggaaatagaa ccggttttga tttgacctgt atttaaagca acagccaaat cggcaataaa
     2641 agtatctccc gtttcaccgc tacggtgact tacaacggtt gtccaacccg ctttatgagc
     2701 catatttatg gcatctatgg tctcggtaac ggttccgatt tgattcaatt taatcaaaac
     2761 actattggcg atgttatctt taatgccttt agcaataatt ttgggattgg taacgaaaac
     2821 atcatcacct acaatttgaa ttttactgcc caatttggca gtcatttttt tccagccagt
     2881 ccagtcattt tctgccaagc catcttcaat tgagcaaata ggatatttgg caatcatact
     2941 ttcataatat ttaatcattg catcgctgct aacttttttt ccttcaaaaa gatacttacc
     3001 gtctttataa aaactgctgg cagcagcatc aatagccagg taaatatctt ttccgggaat
     3061 gtaacctgct gtcttaattg cctctacaat tacgataaag gcctcttcat tggatgcaag
     3121 attaggtgca aaaccacctt catctccaac ggaagttact aaaccgcgtt ttttaagaat
     3181 tgccttcagg gagtgaaaaa cctcagcatt catttgtagc gcttgtttga aactttttgc
     3241 acccaatggc ataatcataa attcctgaat atcaacattg ttatctgcat gcgcgccacc
     3301 attgagaata ttactcattg gtacaggaag ggttttggca ttaacacctc ccagataacg
     3361 atataaagga atattcaatt ccaaagcgga agctctggca acagccatag aaacagctaa
     3421 aatagcatta gcacccagtt tggctttatt aggagttcca tccatatcaa tcataatttt
     3481 atcaatttga ccctgcaata atgaatcagt tccccataat tcatcactta tattgtctat
     3541 attgctaact gcttttaaga cacctttgcc tccatagcgt tttttatcgc catcacgaag
     3601 ttctatggct tcgcgttctc cggtagatgc accactggga actgctgcta ttgccataat
     3661 ctcactatct aaatgaactt ctgcttccac tgttggattt ccgcgtgaat ctaaaatctc
     3721 gcgtccgata atgtaaagta tatctgacat tttttcctcc tgtttatttt taatcctcat
     3781 atactgaata aggttttttt ggcaaggaaa atttgggaag acgagataga aagtagaaag
     3841 gtcttaaggt ctttatttct ttacttctca accctctaaa ccttctaaac tctctaaact
     3901 ttctcaaccc tctaaacctt atcaaccctc taaaccttct caacctactc aacctactca
     3961 accctttatc cgtcatttac gatgttaact cagtgtttca ccttttcacc tttctcgttc
     4021 acctgttaac caattccacc atttctgcta ttttatcata acaacttgag agagaatgaa
     4081 atacagagct tgtgtttcat agaatataga caagggattt tgtgttgcat aaaacgctaa
     4141 agatgaggca caaacactct atattattta tttattgtta gttatacatt tatttaacaa
     4201 tgttataaag agctttgaac ttaaacaatc ctggcatttg tcccctgctc ttttgtattt
     4261 ctgtcacgat tttctcattt tttaatatat ttactattct tatgatggtt gctttaggaa
     4321 tcattgaagc tgtggaaaga ccgttaatac tgaaaagagg tgatataaaa agcgcatcag
     4381 tcagctgcat tgaatatttg gaaccggtaa tttcaggaat tgtaattttc atttgatcat
     4441 acaaattttg gatagctcct gctttttcaa tattcatttt tgcctgttca ataactgctt
     4501 tcaggaaaaa agaaatccat ccgttccagt cattttttgc agtaatatta tttaggtgct
     4561 gataatattc atctctatgt ttttctaggt aagtgcttaa gtaaaatacc ggcttgctta
     4621 tgatattttt atagaacaga aagagaggga tgagaattct tcctattctt ccatttccat
     4681 ccaggaaagg gtggattatt tcaaattgtg ctttgattat tgccaattga acgattgtat
     4741 ctttttcttc ataatggatg tatttttcca gattatccat agcaggcatc acctcttcgg
     4801 gagagggagg gatataggtt gcctgggaaa tatctgcacc tggaggacca atgtaatttt
     4861 gaacttttct aaattctccc ggagttttat tcattcctct aacgctgttc atcaatatgg
     4921 aatggacatc tttgatcaaa tttaaggata gaggtatgga ttttaaccgc tcaactgcat
     4981 aattaattgc ttcccgataa tttaatatct cttgaatatc agcatttctt tcaggagata
     5041 gtgtcatttt aggatttgct tcatagcgca agacctcatc caaggttgcc tgtgtgcctt
     5101 caattttaga agataaaact gcttcctgag tagtgagagg cgataaaaac agattgggat
     5161 ttactattgt ttgtagcatc ccttcatatt tacctaacaa attatgagct tggctaataa
     5221 aacttatctg tctttcccaa tcaatacagg aaagaggaag cttttccggc ttaaaaggtt
     5281 tcatcttgtt tttcccatac ttaaaattct ataattgctt tcttatttaa tgataagtta
     5341 ttgtcaatgt ttttattagt tgagaggtct gaaggtctgg aggaggaaaa gtcaggagtc
     5401 gtcactgcct ttaataattt tacacatctc acgaatttat ttggcagtga ggactcataa
     5461 cgagttagcg cgcagatttt gctgatttta ttataataat tttgcctttt ctttgtctag
     5521 cccgtgtttc actttccact tcaaccgttc atccaaaaag ggcttttcct tttcggaaaa
     5581 gcccttttct ttattttaga cgggattata ttaatacata tcagccatat taggattggg
     5641 catagggaca gctggctcag gttctttaat atcggtaata atgcattccg tagttaacat
     5701 caaaccggca atggaagcag cgttttggat agctgagcga acaaccttag cggggtcaat
     5761 aattccggct ttgaagaggt cttcaaattg accattgctg gcattgaatc ccatatggat
     5821 atcgcgaaag ctctttagtt tttcaacaac tacagcgcct tcttcacctg cgttagcggc
     5881 aatttgataa gctggttttt ccagtgcttt agacaaaata tccacagcca ttttttcttc
     5941 gtaggaaagg ccttttagtt gttttagggc tttagcggct tgaattaaag taattccacc
     6001 accgggaaca ataccttctt caacagcagc ccgggtagca tggagagcat catctacgcg
     6061 agctttcttt tccttcattt ctgtctcggt agcagcaccg attctgagga cggcaactcc
     6121 gctggaaagt ttggcaaggc gttcttgcaa tttttcttta tcgtaatcgg aagttgtctc
     6181 ttcaatttga gctcttattt gtttaattct accttcaatc tcttcaggat taccagctcc
     6241 ttcgcggata gtggtatttt ctttttccac aataatcttt ttagcgcgtc ccagatcgct
     6301 cattgtagcg ctatcaagtt ttcttcccat ttcttcagaa attaaagttg caccagttaa
     6361 aacggcaata tcttccagca tagcttttct gcgatcaccg aaaccgggtg ctttaacagc
     6421 aaccacattc aggacaccac gcagtttatt cactactaat gtggcaagtg cttcgccttc
     6481 aatatcttct gcaataataa gcaggggacg tccattttgg gaaacttcct gtaagatagg
     6541 aagaagttct ttgagagctg ttattttctt gtcgtataaa aggataaagg cctcttccag
     6601 ttcagcaatc atcttttctg cattagtgac aaaataggga gagatataac ctctatcaaa
     6661 ctgcattcct tccacttttt ccaaaccggt atcaatggat tttgcttctt caatattgat
     6721 aatgccttct ctaccaacac tatccatagc ttcagcaatc agagctccaa tttcaggatc
     6781 gttattagct gaaattgcag caatttgtgc aatttcttca ctgctcttga tttccttgct
     6841 gaattccctg attttatcca ctacaacttt agttgccttt tccaaaccgc gtttcagata
     6901 cattggatta acgccggcag ttacatgttt cagaccttct tcaataattg cctgggctaa
     6961 aattgtagct gttgtagttc catcaccagc tgtgtcatgg gttttttctg caacttcttt
     7021 gcagagttga gcacccatat tttcgtattc attttccaat tcaatttctt tggctatggt
     7081 cacaccgtcg ttagtgattg tgggagagcc aaatttcctg tccaaaacaa catatctacc
     7141 cttaggacca agggtaactt ttacggcatc agccagttta tccacaccgc tttttaaact
     7201 tgtgcgggcg tcatgtgcat actgcatttg ttttgccatc ttgttttcct ccataatttt
     7261 agtattttaa ttttggtttt cttagcagtc aatacaattg agtgctaata ttattatagc
     7321 cgaaattctg tcaagcgcaa attaaaaatt ttttgtcttt cggctttatt tttctctttt
     7381 caatggggct gtcccgaaac taaaacttag ttatgagaca gtaaatataa gccattttaa
     7441 agaataatct cctctttagg atacatcttt gtatacct
//
LOCUS       DAOOZV010000073         7472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8930_length_7472_cov_74.643252, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000073 DAOOZV010000000
VERSION     DAOOZV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8930_length_7472_cov_74.643252"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            774..1517
                     /locus_tag="PLW08_06845"
     CDS             774..1517
                     /locus_tag="PLW08_06845"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPL52353.1"
                     /translation="MNYKKERERISNFIRDYCRKSRFENLVIGLSGGVDSALVSALAV
                     QAIGREHLFAFCLPSRESNPESVKDAFLVAECLDISMQTINIDELTESYFKKYAPEAS
                     PLRKGNWMARIRMNILYDQAAKHNALVIGTSNRSELLVGYFTQFGDSACAFEPIAHLY
                     KTEVWELARELCLPEKVINKTPTADLWSGQSDESELGISYPILDSVLKYLTEGERKPE
                     VAEEIIVKVQKMMDNSSFKRKMPPFLERQ"
     gene            1518..2693
                     /locus_tag="PLW08_06850"
     CDS             1518..2693
                     /locus_tag="PLW08_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52354.1"
                     /translation="MLKLFKSSRCQSCYTERAIRVCPRKRKNICWQCCNETRCDTRCP
                     ENCDYAPKKEESKPFPSFKADSRAEAENAIKCHIDLWTGRENPFLKNKTPAKEASENP
                     QGMLAWLSGFQYPVYFPMDYLLKKLDLPFTPAEKKEDPEDCVEKWMKTLIQLKWNELR
                     QYSINQSSMEDLAERYTELIQAIPQLGKVSTYSILYSGLGEDGISALVYMELNHKIDW
                     TLVLSNITGSWKIRQNIAGSPQAYFKQNQVYTAIAEALGKADDAKVWELINSSLKIYP
                     DSPDLYYYRALYWQLVKQREQAAVDFFNAIALDNSWTEPYLHLSAHYFVKGDYEQAKI
                     WLEELKILQPDDPNVLNNLAAAYAGNGETEKAKELWQEIVQKYPAFEYARKNLEKLQ"
     gene            2690..3796
                     /gene="ribD"
                     /locus_tag="PLW08_06855"
     CDS             2690..3796
                     /gene="ribD"
                     /locus_tag="PLW08_06855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPL52355.1"
                     /translation="MTDAQIKQYMQRAIKVAEKARGKCSPNPFVGSVIVKNNQVISEG
                     WTLEYGSDHSEIQALKKAGKKAKDATLFVTLEPCSHYGKTPPCAQAIIDAGIKEVYIG
                     IYDPNPLVKGKGIAMLKEAGIDVHYGFLEEKIRTQLECYLCYIQKERPFVTLKTALTL
                     DGKYAAEDGTAHWITGKKAREYAHQLRSENDVVLTGIKTILFDDPRLNVRLPGNYKQP
                     LRVVLDPYLKIPVLSYFVSISAKFPGMIITSPDNLQTDKADILRQKGVKIEALETKDG
                     HFNLHEVLNLLYKMNYYSVLLETGSGIAEAFLKEELIDKIIFIYGAKILGGTKSPLPN
                     LGIESIYKALYIWKTSCRKIENDVVVTAYPVYHI"
     gene            3965..4360
                     /locus_tag="PLW08_06860"
     CDS             3965..4360
                     /locus_tag="PLW08_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPL52356.1"
                     /translation="MKIIHYDEVELEPVTAEGAEKAQIRWLIAQKDGAPTFAMRMFEI
                     EPGGHTPHHQHKWEHEVYCLSGKGALVTDRGEMAFGADDAIFVDPDILHSFKNTGNET
                     LKFLCLIPHEKPIIKKALNPFADEEANNC"
     gene            4533..4709
                     /locus_tag="PLW08_06865"
     CDS             4533..4709
                     /locus_tag="PLW08_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52357.1"
                     /translation="MAKNNRGKGLKKVPNHGRGECPVCHRTGIKLLYEVKVGENTYKV
                     CKSCKGIAAEKLAG"
     gene            4770..5552
                     /gene="rsmA"
                     /locus_tag="PLW08_06870"
     CDS             4770..5552
                     /gene="rsmA"
                     /locus_tag="PLW08_06870"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPL52358.1"
                     /translation="MKALKALGQNFLTDKSIAEKIVALGELQPEDSVWEIGPGTGILT
                     DEIIKNNVQLRAFELDRRLYKYLSARYQNRIILEQTDILQADWNSLIRKDGTPLKLIA
                     NIPYQITSPLLALLEEYSQFFSRIVLMVQKEVAERLSAKPGNKNYAPLTIRLRLVFDI
                     SSKIQVSREKFSPMPKVDSAVILLLPRENKPVIKHPEHFHKLLHAAFVHRRKTLANNL
                     IPVLGKEKTEELAKLSHIDFGKRGEELAEGDFILLSDCLADL"
     gene            5503..7371
                     /locus_tag="PLW08_06875"
     CDS             5503..7371
                     /locus_tag="PLW08_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52359.1"
                     /translation="MQKGILFCLVIVWLIYSPFLAAQTQGLDWTIYSSQQALSIENYM
                     QYKYFPTAGTNLIISSKSNLENRLNFNQEAKNADLNVGFQIARKKFLHTLSSGYLTFY
                     DASDLEPSAYVNKTATLGYQINYTPVESLNIGIFSKGIFRNEQDRYVSGNLLSSEGYW
                     IGSDIHYATYFSNSIAGIGTTGERKKMDWESFDFAQVNANYNFYSNYLNVDCLANYSY
                     RSEDIYILTAPRQNENRSNYSLSDNQLRRNLTFAGSVQYYPDDKVQIQLRDDYSQRKT
                     SYSQNEIRNSTDYYNLAQLQFDYQLFPSLVWQNNLRHTYAIKDYYYGLNTRHTENRHF
                     GSRVNWEYSDNDSLIVDYAIDLQIIKFPEDNNQWDNDLLSHNLLWGWKHYWHERILLG
                     NWFGYGFSKDVYLDSLLSANNKRINSWTLSPECNILLGDRLAFFQVYKIRADYSNYIY
                     KTGKSNTLYRQLSYQYDLVFDTYPLIARSLDPRWLKLPFRNSPDNAFMIDLGFAYEEN
                     QYAQKRKEFYELQTKNRRWTANIDFRYDIRTFYFSFSPQYSWGTWQELTFNLNSAWLF
                     NNNSLLEFKLSPFIDDFEEIEPLSLHNFSINKFFRCVKQCSESADWRLSTTLKLRF"
BASE COUNT         2473 a         1285 c         1540 g         2174 t
ORIGIN      
        1 taaaaggtct gtgataaaca aaatgataag gttgagaggg ttgagaaggt ttagagggtt
       61 tagagggttg ataagttttt gatggtttaa atgttttaga tggttgagat gccttaaaag
      121 cccaaagggc gaaacaatga tagcgatggt ggcgtaagtt ttattgtaaa agaaggttta
      181 caattttaat tcctttattc tttttcaccg ggttaaaacc cgttgttaat atctgtcgtt
      241 cctacggaac tcattcttaa caaaagagat aagatgtttc agttatcatt tttcaccggg
      301 ttaaaacccg ttgttagtat ctgtcgttcc tacggaactt actcttaaca aaagagataa
      361 gatgtttcaa ttgtactttt tgacaatctg actttaaaaa tactcacatt gataatctga
      421 cttatacaat ctgacttata caatctgact tttaaaattt cacttttaca atctcacttt
      481 gaagaaactc actttgataa tcacactgct tctgctctca catcattctt ttttcatttt
      541 tccctttccc aatagcattg tcttagcaat acggactcat tgctaatgcc atccgtaatg
      601 ggtaagcaat gggtaagcaa tgctattaag gaagaagtag caattgtttg tagggcaatt
      661 tgttatgtta agttagagaa ataaccccaa aagagaattt gcttgacgga aaaaagagaa
      721 aaaaagataa tgataaaaaa gtaatagata aatggaagga agcgaaaatc ataatgaatt
      781 ataaaaaaga aagagagagg ataagcaatt ttattcggga ttactgccgg aaaagcagat
      841 ttgaaaatct tgtgataggt ctttccggtg gcgtggattc agctctggta tccgctttag
      901 ctgttcaagc aattggcaga gagcatttat ttgccttttg tttaccttcc cgggaaagca
      961 atccggaaag cgttaaagat gcttttttag tggcagaatg tttagatatt tctatgcaaa
     1021 ccataaatat agatgaactt acggagagtt attttaaaaa atatgctccg gaggcaagtc
     1081 ctttacgcaa agggaattgg atggcacgaa tccgaatgaa tatcctttat gaccaggcgg
     1141 caaaacacaa cgctttagta atcggaacga gcaatagaag cgaactttta gtcggttatt
     1201 tcactcaatt cggagattct gcttgtgctt ttgaaccaat agcacatctt tacaaaactg
     1261 aggtttggga actggcaagg gaattatgct tgcccgaaaa ggttatcaat aaaacaccta
     1321 cagctgattt atggtcaggt caaagcgatg aatccgagct ggggatcagt tatcctattt
     1381 tagatagcgt cctgaaatat ttaacggaag gagaaagaaa accggaagtg gcggaagaaa
     1441 taatcgttaa agtgcagaaa atgatggata attcaagttt caagcgtaaa atgcctcctt
     1501 ttttagagag gcagtaaatg cttaaattat tcaaaagctc gcgttgtcaa tcctgttata
     1561 cggaacgggc aattagagta tgtccccgca aaagaaaaaa catttgctgg caatgctgta
     1621 atgaaacacg ctgtgataca cgctgtccgg aaaattgtga ttatgctcct aaaaaagaag
     1681 aatcaaaacc gtttccttcc tttaaagccg attctcgcgc cgaagcagaa aatgctataa
     1741 aatgccatat agacctttgg acgggtagag aaaatccttt cttgaaaaat aaaactcccg
     1801 ccaaagaagc cagtgaaaac ccgcaaggaa tgcttgcctg gttgagtgga tttcagtatc
     1861 cggtatattt tccaatggat tatctgctga aaaagttaga tttgcctttt acccctgctg
     1921 agaaaaaaga agaccctgag gactgtgtag aaaaatggat gaaaaccctt attcaactga
     1981 aatggaatga attacgacaa tatagtatca atcagagctc tatggaggat ttggcagaac
     2041 gctatacaga actgattcaa gccattcccc agctgggtaa ggtctctact tacagtattt
     2101 tatacagcgg attgggtgag gatggaataa gcgctctcgt ttatatggaa ttaaatcata
     2161 aaattgactg gactttggtg ctgagtaata taaccggcag ctggaaaatc agacaaaata
     2221 tagcaggcag tccgcaggca tattttaaac aaaatcaggt ctatacagct atagcagaag
     2281 cacttggcaa agctgatgac gctaaagttt gggaactgat aaacagcagc ttgaaaattt
     2341 atcccgatag tcccgatctc tattactatc gcgctttata ctggcaattg gtaaaacaaa
     2401 gagagcaagc ggcggtggat ttctttaatg ccattgctct tgataatagc tggaccgagc
     2461 cctatttaca tttaagcgcc cattattttg tgaaagggga ttatgaacag gcaaaaatct
     2521 ggctggaaga actgaagatt ttacaaccag atgaccccaa tgtgctgaat aaccttgccg
     2581 ctgcttatgc tggaaacgga gaaacggaaa aagcaaaaga actttggcag gaaatcgtgc
     2641 aaaaatatcc cgcttttgaa tatgccagga aaaatttgga gaaactgcaa tgaccgatgc
     2701 ccaaataaaa caatatatgc aacgagcaat taaagttgca gaaaaagcca gaggtaaatg
     2761 ttctcccaat ccctttgtgg gttctgttat cgtgaaaaat aatcaggtga taagtgaggg
     2821 ctggactttg gaatatggaa gcgatcattc agaaattcag gcacttaaaa aagcaggcaa
     2881 gaaagccaaa gacgctactt tatttgtaac tttagaaccc tgttcccatt acggtaaaac
     2941 accgccctgt gctcaagcta taattgatgc cggaattaaa gaggtctata ttggaattta
     3001 tgatcccaat cctttggtta aaggcaaagg gattgcgatg ttgaaagagg cgggaattga
     3061 tgttcattac ggctttttgg aagaaaaaat aagaacccag ctggaatgtt acctttgtta
     3121 tatacagaaa gaaagaccct tcgtaacttt gaaaacagcc cttactctgg atggaaaata
     3181 tgccgctgaa gatggcacgg cacactggat aactggaaaa aaagcccggg aatatgccca
     3241 tcaattacgc agtgaaaacg atgtggtttt aaccggaata aagaccatcc tttttgatga
     3301 tccacgactg aatgtgcggc tcccgggaaa ttacaaacaa ccactgagag ttgttttaga
     3361 cccttatctt aaaatacctg tgctttccta ttttgtaagt atctctgcta aatttccagg
     3421 aatgattatt acctcgcctg ataatctgca aacagataaa gccgatattt taagacaaaa
     3481 aggggtaaaa attgaagctc tggaaactaa agacggacac tttaacttac acgaggtctt
     3541 aaatttatta tataaaatga attattacag cgttctgctg gaaacaggca gcggaatagc
     3601 ggaagcattt ctcaaagagg aattgataga taaaattatc tttatctacg gagccaaaat
     3661 tttaggcgga actaaaagtc ctttgcctaa tttgggcatt gaaagtatct ataaagcatt
     3721 gtatatatgg aaaacaagct gccggaaaat tgaaaacgat gttgtagtta ctgcttaccc
     3781 tgtctatcat atttaagaat ttgtctttag ttcttggcgc tttagtttat ctattgtata
     3841 atttttataa tggaaatgca gttaggatgg taaattttat ttagattccc gcttttgcag
     3901 gaatgccaaa ggacttttaa ggcaaaaatg atattacaaa ataaattccg agaaggagga
     3961 aacaatgaaa attatccatt acgatgaagt agaattggaa cccgtaactg cggaaggtgc
     4021 tgaaaaagca caaatccgct ggctgattgc ccaaaaagat ggcgctccaa cttttgcaat
     4081 gaggatgttt gaaattgaac ctggtgggca tactccccac catcaacata agtgggaaca
     4141 tgaagtttac tgtctttccg gaaaaggtgc cttagttaca gaccggggag aaatggcttt
     4201 tggagctgat gacgcaattt tcgttgaccc tgatatttta cattcattca aaaatacagg
     4261 aaatgaaaca ttgaagtttc tctgtttgat tccccatgaa aaaccgataa tcaaaaaagc
     4321 gctaaatccc tttgccgatg aggaagcaaa taactgctaa aaaaccggaa ggttgatgga
     4381 ctttgttaac cacttaaaaa gtaaggagtc gtcactgcct ttaataattt cacaatccct
     4441 caattttatt tggtagtgag gactcgtgat gactttagtt aaaggtgaga agcatagaat
     4501 tataaaataa taaacagaaa aaggagaaaa caatggcaaa aaataatcgc ggaaaaggac
     4561 tgaaaaaagt tcctaatcac ggtagaggag aatgtcccgt ctgccataga acgggtataa
     4621 aacttctcta tgaggttaaa gttggggaaa atacctacaa ggtctgcaaa agctgtaaag
     4681 gtatagctgc tgaaaaatta gctggttagg taattaaatt ctgctctcgc gggaaaaata
     4741 ccttttattt accgaaagca ctcttttgta tgaaagccct gaaagcactc gggcaaaatt
     4801 ttcttaccga taaatctata gcggaaaaga ttgttgcctt gggtgaatta caaccggaag
     4861 atagcgtttg ggaaataggt cccggaacag gtattttaac tgacgaaatt atcaaaaaca
     4921 atgttcagtt aagagctttt gaactggaca ggcgattgta taaatacctt tccgcacgct
     4981 atcaaaacag aataatattg gaacaaacgg atattctgca agcggactgg aattctctta
     5041 tacggaaaga tggaactccg ctcaaattaa tagccaatat tccctatcag ataaccagtc
     5101 cgcttttagc tcttttggaa gaatattccc agttcttttc ccgaattgta ttgatggtgc
     5161 aaaaagaagt tgcagaacgg cttagcgcca aacctggaaa taaaaattat gctcccttaa
     5221 ctatccgtct gcgtttggtt tttgatattt ccagcaaaat acaggttagc agagaaaagt
     5281 ttagcccaat gcctaaagtg gattctgcgg ttattttgct gcttccgcga gaaaataaac
     5341 cggttattaa gcatcctgaa cattttcata aacttttgca cgcggctttt gttcatcgga
     5401 gaaaaacact ggctaataat ttgattcctg ttttgggaaa agaaaaaaca gaggaattgg
     5461 caaagctttc gcatatagat tttggcaaaa gaggagaaga acttgcagaa ggggatttta
     5521 ttctgcttag tgattgtttg gctgatttat agtccttttt tagcagctca aactcaggga
     5581 ctggattgga caatatattc cagtcagcag gcattaagta ttgaaaatta tatgcaatac
     5641 aaatattttc ctactgccgg cacaaattta ataatcagca gtaaaagtaa tcttgaaaac
     5701 cggctgaatt tcaatcagga agccaaaaat gcggatttga atgtcggttt tcaaatagca
     5761 cggaagaagt ttttgcacac tctcagctcg ggatatttaa ccttttatga tgccagtgat
     5821 cttgagccct cggcttatgt aaataaaact gccactttgg gttatcaaat aaactatacg
     5881 ccggtggaga gtttaaatat aggtattttc agcaaaggca tattccgcaa tgaacaagat
     5941 cgctatgtta gcggaaatct gctttccagt gagggttatt ggattggttc cgatattcat
     6001 tatgccactt atttttccaa ttccatagcc ggtataggaa caactggcga acgcaaaaaa
     6061 atggattggg agtcctttga ttttgcccaa gtgaatgcta actacaattt ttacagtaat
     6121 tatttgaatg tggattgcct tgccaattac agctacagaa gcgaagatat ttacatttta
     6181 actgctccga gacaaaatga gaataggagc aattattcgc tgtcggataa tcagttacgc
     6241 agaaatttaa cctttgcagg atcagttcaa tattatcctg atgacaaagt tcaaattcaa
     6301 ctaagggatg actatagtca aagaaaaacc agctacagcc aaaatgaaat tcgtaatagc
     6361 acagattatt ataatctggc tcaactgcaa tttgactatc agctgtttcc aagtttggtt
     6421 tggcaaaata atttaaggca tacttacgcg attaaggact attattatgg cctcaataca
     6481 aggcatacgg aaaacaggca ttttggcagt cgtgttaatt gggaatatag tgacaatgat
     6541 tctttaattg ttgattatgc tattgatttg cagataatta agtttccgga agacaacaat
     6601 caatgggata atgacctgct atctcataac ttgctttggg gatggaaaca ttattggcac
     6661 gaacgcattc ttttgggaaa ctggtttggc tatggcttca gcaaagatgt atatttagat
     6721 tccctgcttt ctgcaaataa taaaaggatt aacagctgga cgctatctcc cgaatgtaac
     6781 attttgctgg gagaccgttt agcttttttt caggtatata aaataagagc ggattatagt
     6841 aattacatct ataaaacggg taaaagcaat actctttatc gtcagttgag ttatcaatat
     6901 gacctggttt ttgatactta tcctttaata gctcgttctt tagacccgcg ttggctaaaa
     6961 ctgccatttc ggaatagtcc cgataatgcc tttatgattg atcttggttt tgcttatgaa
     7021 gaaaatcaat atgctcaaaa gagaaaggaa ttttatgaat tgcaaaccaa aaacagacgc
     7081 tggactgcca atattgattt ccgttatgac attcggactt tttactttag tttttcacct
     7141 caatattctt ggggaacctg gcaggaattg acttttaatc ttaattccgc ctggcttttt
     7201 aataataatt cgttgttgga attcaagctt tctccgttta ttgatgattt tgaagagatt
     7261 gagcccctaa gtttacataa cttcagcatc aataagtttt ttagatgtgt taaacaatgt
     7321 tctgaatcag cggactggcg tttgtccact accctgaaat tgcgtttttg attgacaaaa
     7381 taagcgagag aaaaatctct gaatttgcca ggcgcaatgc aataactgca agtgaactat
     7441 gtcagatcag gaatgaagca gcattaagct tt
//
LOCUS       DAOOZV010000074         7463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_8951_length_7463_cov_54.575729, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000074 DAOOZV010000000
VERSION     DAOOZV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8951_length_7463_cov_54.575729"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..2523
                     /locus_tag="PLW08_06880"
     CDS             241..2523
                     /locus_tag="PLW08_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPL52360.1"
                     /translation="MRLNRNQLARKIMLVAGLCLVTIIMWAKVVEPENALLAAKNYLQ
                     EAGLKSPIYAGNAELFVYSSGKLLPLEFNKALEFEPTLYFINYANGNYAIVSAEDNFY
                     PVLAYSDEGLTSINNLPPAFYYWLENYSVQIEQIREAKIAYPENVQLWQSLIEGSYRN
                     AFRTERSVRPLVTTLWDQGWPYNALCPVDPQGSGGHVYAGCVATAMGMVMKYWNHPQT
                     GVGSESYYCPGYGYQSANFGNTTYLWDQMYDTAGATPAEYLPIATLLYHCGVAVHMAY
                     SVEGSGAQSTDAAVAFVDHFRYPNAQYVMKNSYTDANWNNLLTSQIDNGIPVYYSGYD
                     PVEGGHAFVMDGYDTANHFHFNFGWSGSGNGYFYTSNPGGFTNNQSAIINIIPENYSI
                     STVPVKLNAHDTTAGDNFTVTVKTNPILGSWNITHYDFVLYYDSEFIDYIGYSTTGTI
                     SENGTITVVENPAGIISVDWNSTNYIFGGGVLINFTFRTRDMGDFLFDITSMHYNTTP
                     VSNISYVMIHSYAPVNNISESRILLTNIMNLAYNAIGTTQMNTTYLLPSWNITHFQYH
                     LNYNPAKIEYFDIVTEGTISANCEVNVDSSNPGVLNISGNSTVPLIGAGALMKIRFKA
                     IGNTGSISVTQISISDFLYNNVAISDVGTANVVLSAYTANEDEIVAVPEPKLEIYPNP
                     FQDSAMLKFTGTNKAPVRIHIYNIKGQLVKELLISDPLNSQISWNRSDVKGKTVADGI
                     YFLHWQQGEQSGINKVLVIK"
     assembly_gap    2831..2930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2931..3536)
                     /locus_tag="PLW08_06885"
     CDS             complement(<2931..3536)
                     /locus_tag="PLW08_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL52361.1"
                     /translation="MAKVLFYLERKLGAWLLLLLRKTLKFKVINQESSDNIRCIYMFW
                     HRNLLMMTLQRVYHGAAVMVSSSKDGELIAGPLWELGYIPVRGSSSFKGSSAMKEMIK
                     ISQKISLAITPDGPKGPCYTFHPGLFQIAYLAKIPIVAVAVNADKEWQFNSWDRFRFP
                     KPFAKLTMIYSDPIWVKSKDDFATAEREIRDFLAKNEKDTFF"
     gene            complement(3529..4674)
                     /gene="lpxB"
                     /locus_tag="PLW08_06890"
     CDS             complement(3529..4674)
                     /gene="lpxB"
                     /locus_tag="PLW08_06890"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPL52362.1"
                     /translation="MIRKESYKIFWLVGESSADLHSELVMKALNDKFGNLTHIGIGGP
                     RMQRQGLKTLFPFEKFAVMGFVEVVKHLFFFLKVQRKLGKLFSTEKFDLAILVDYPGL
                     NLRIAKMADEMRIPVLYFICPQFWAWKHKRVYQLKDSVRYVACILPFEEELLKIHNIN
                     CSYVGHPIAEEITFELDRGSFARFFGLDPNKKWIGFFPGSRNNEITKMLPVFLQTAQK
                     WNPTEYQMLFSKSHSVNHQLYMHLIEGHKNIKLIDGYNYEMMKYCELLICTSGTVTLE
                     AAYIGTPLVICYKGSYLSYLIGRIFVRIKHIGLPNIVLDADLLPELIQGEMKPDNIYK
                     AGMQILSDPEKNAQIRKELFKLRAMLSDKHPSQEMPKIVRYLLDTYG"
     gene            complement(4671..5243)
                     /locus_tag="PLW08_06895"
     CDS             complement(4671..5243)
                     /locus_tag="PLW08_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPL52363.1"
                     /translation="MKLDNQLKKILLILLIVILSQPLFCNTTADKTVAWLKAKGVAPE
                     LIVVIISMIPVVELRGSIPVAILLFNIPWLEAAVLSIIGNMIPVPFLLLLIDWFLGLI
                     SKVKPGRKFTEWLFTRTRRKGKSIEKYEEIGLVVFVGIPLPGTGGWTGALAANIFGLR
                     FWRSMLFIFLGVIMAAIIVTALSLMGTLAL"
     gene            5726..6085
                     /locus_tag="PLW08_06900"
     CDS             5726..6085
                     /locus_tag="PLW08_06900"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HPL52364.1"
                     /translation="MNLTNCPICKGELKIRVLHCPHCEISFQGDFENNWLAELDAGQL
                     EFIRLFLLVQGNLKELQNQLGISYPTVKNRLAEIIGIITKKEPVTDTVIDVLSDLEEG
                     FINVDEAINMITQRREK"
     gene            6082..7458
                     /locus_tag="PLW08_06905"
     CDS             6082..7458
                     /locus_tag="PLW08_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPL52365.1"
                     /translation="MSKINLEKKWVFEPLHKVVIENHLAKLLFQADEGQEVNMEGIIN
                     FSHSQDTFQAEDYIQSEYQDGILKIILQDIDSDEVKDAVFTVTIPEGVYLKVKTDNYP
                     ISLNNLKNKLKVLNENSPIYLQNCQGDMHLENENGLIRLSDCEGNIDAKLENGPLSAS
                     KISGQTLHLENENGPIKVRMASFTEVELYSENGPIFYETIPVENGNFQFKTENGSINL
                     VLPNNFDFTLEATTQWGRVKTSFDLPITFNDNIYTMINGEGTSQIKAISDNGTIKINA
                     ENRLNLDFVMNKLEQIKIALQKVNSEAEKQKVVEMVNKITTYINRLADSIKEEKIKEK
                     ITSATSKLKDLVVNFDFRETNDKVIKSVEDIGSQIQDAFKEGIKNIKESVDDLKKHRF
                     HTESVAAYVKKILDSPQIKPYLGGEHKKKEKENIADRSRIKILEMLEAGKITAEEAER
                     LLKAIGKE"
BASE COUNT         2507 a         1248 c         1387 g         2221 t
ORIGIN      
        1 aggcaataaa aatattaggg tttgcctgca taagataatc gcgttattgt ataaatagga
       61 taaagaaata tatacaggta atcagataaa cgatggtaat agaagtaaga gagaacaagc
      121 ggacaaaaaa acttaaagag agtaaagaac taagttaaag taaaggaata aggagaggga
      181 tggaataatt cgtgcataaa ataacatata taaataaata atatatatcc gggaggataa
      241 atgagattaa atcggaatca attagcgaga aaaattatgc tcgttgcagg attatgcctg
      301 gtcactatta ttatgtgggc taaagtagta gagccggaga atgctttgct ggctgcaaaa
      361 aattaccttc aagaagcggg actaaaaagt ccgatttatg caggtaatgc cgaactattt
      421 gtttatagtt cggggaagct attgcctttg gagttcaata aagcacttga atttgagcca
      481 acactctatt ttattaatta tgcaaacggt aattatgcta ttgtgtccgc cgaggataat
      541 ttctatcctg ttttagcata ttcagatgaa gggttaacca gtatcaataa cctgccacct
      601 gctttctatt actggttgga aaattattca gttcaaattg aacaaattag agaagcgaaa
      661 attgcatatc cggaaaatgt tcaactgtgg cagagcttaa ttgaaggttc ttataggaat
      721 gcttttagaa cagagcgttc tgtaagacct ttagtgacga cgctttggga tcaaggatgg
      781 ccctataatg cgctttgtcc tgtagatcct caaggttctg gaggtcatgt ttatgcaggt
      841 tgtgtagcta cagcaatggg tatggttatg aaatattgga atcatcccca aaccggagta
      901 ggtagtgaaa gttactattg tcccggttat ggctatcaaa gtgctaattt cgggaacact
      961 acttaccttt gggatcaaat gtatgataca gcaggagcga caccagctga atatttgccg
     1021 attgcgacat tattatatca ttgtggtgtt gctgttcata tggcatattc tgttgaaggt
     1081 tcaggagctc aaagtacaga tgcagctgtg gcatttgtag atcatttccg ttatcctaat
     1141 gcacaatatg taatgaaaaa cagttatacg gatgccaatt ggaataattt attaacttct
     1201 caaattgata acggcattcc tgtttattat agtggttatg atcccgtaga agggggtcat
     1261 gcttttgtaa tggatggtta cgatactgct aatcatttcc atttcaattt tggctggagt
     1321 ggttcaggta atggttattt ttataccagc aatccaggtg gttttactaa caatcagagt
     1381 gctattatca atattattcc ggagaactac agcatttcta ctgttccggt aaaacttaat
     1441 gcgcatgaca caactgcggg cgataatttt actgttaccg ttaaaacgaa tcctattctg
     1501 ggtagctgga atataacaca ttacgatttc gtgttatatt atgacagtga atttattgat
     1561 tatataggtt attccaccac gggaactatt tctgaaaatg gaactattac cgtagttgaa
     1621 aatcctgccg gaattataag cgtagattgg aatagtacga attatatctt cggtggtgga
     1681 gtattgatta attttacatt tagaaccaga gatatgggag attttctgtt tgatataacg
     1741 agtatgcatt ataatactac accggtttca aatataagct atgtgatgat tcattcttat
     1801 gctcctgtaa acaacatatc tgagagccga attttgctta ccaatattat gaatctggct
     1861 tataatgcaa ttggtactac acagatgaat actacttatt tgcttccttc ctggaatatt
     1921 acacatttcc aatatcatct taactataat cctgccaaaa tagaatactt tgacatcgta
     1981 acagaaggaa cgataagtgc aaattgtgaa gttaatgttg attcatccaa tccaggtgtt
     2041 ttaaatattt caggtaattc aactgttccg ctaattggag ccggagctct aatgaaaatt
     2101 agattcaaag ccataggtaa tacaggttct atatccgtca ctcagatttc tattagtgat
     2161 ttcttgtata ataatgtagc aatttcagat gttggaactg ccaatgtggt actttcagct
     2221 tatacagcta atgaagatga aattgttgct gtgcccgaac cgaaactgga aatctatcct
     2281 aatccttttc aggatagtgc aatgctaaaa tttacaggaa ccaacaaagc tcctgttaga
     2341 attcacattt acaatattaa aggtcaatta gtaaaggaat tgctgataag cgatccgttg
     2401 aattctcaga tcagttggaa tagaagtgat gtaaagggaa aaactgtagc ggatggaatt
     2461 tatttcctgc attggcagca gggagaacaa agcggtatta ataaagtatt ggtaatcaag
     2521 taacataaaa gcgtaattca gtaaaaaaag atttgatgac tcctgattag caaaatatca
     2581 ggagtcatct ttttatatag aagaagagtt tcaatccttt ggtgaagaaa ttgcgtagtt
     2641 gtagccaact ttctcaacat ctaaagggaa tagagataga caatttatcc gaaaaaaggc
     2701 aggaaaaatc atcagacagg aagtttgaaa tttaaataga taaggcaata tctgtcgttt
     2761 ttacggactt tttctgccat ttatgattat agataaagta ttattaagtt ttgtatttaa
     2821 aatgcagtta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaaaagta
     2941 tccttttcgt tttttgccag gaaatcccgt atttcccttt ctgcagttgc aaaatcatct
     3001 ttacttttca cccagatagg atcggagtag atcatagtta gcttggcaaa aggtttggga
     3061 aaccgaaatc tgtcccagga attgaactgc cattctttat cagcatttac agctactgct
     3121 acaataggaa tttttgccag gtaagcaatt tgaaaaagac ctggatggaa agtgtagcaa
     3181 ggtcctttag gcccatcggg agtaatggcg agggatattt tttgggatat ttttatcatt
     3241 tctttcattg ccgaagaacc cttaaaagaa cttgagcccc gaacaggaat atatccaagt
     3301 tcccataaag gtccggcaat taattctcca tccttggaag aagaaaccat aacggctgca
     3361 ccgtgataaa ctctttggag agtcatcatc aaaagatttc tatgccagaa catatagatg
     3421 cagcgtatgt tatcggagga ttcttgattt attactttga atttcagtgt tttccgtaaa
     3481 agcaacagaa gccaggcacc cagtttccgt tccagataaa aaagaacttt agccataggt
     3541 atcaagtaaa taacggacta ttttgggcat ttcctgagag ggatgtttat cacttagcat
     3601 tgctctcagt ttaaatagct ccttccggat ttgggcattc ttttcaggat cgcttaagat
     3661 ttgcattcca gctttataaa tattatccgg tttcatttct ccctgaatca gttccggcag
     3721 gaggtctgca tccaaaacga tattgggtaa accgatatgt ttaattctaa caaatatccg
     3781 acctatcaga taggataaat aagaaccctt gtaacatatt accagaggag ttccaatata
     3841 ggcagcttcc aaagttacag tgcctgaagt gcagatcagc agttcacaat atttcatcat
     3901 ttcatagtta taaccatcaa tgagcttgat attcttatgt ccttctatca gatgcatata
     3961 aagttgatga ttaacgctat gtgacttgga aaatagcatt tgatattcag ttgggttcca
     4021 cttttgagct gtctgtaaaa agaccgggag cattttggtt atttcattat ttcggcttcc
     4081 cgggaaaaaa ccaatccatt ttttattagg atcaagacca aaaaaacggg caaaagaccc
     4141 acgatcaagc tcaaaagtaa tttcttccgc tatagggtga cctacataac tgcaatttat
     4201 attatgaatt ttcagtaatt cctcttcaaa aggaagaatg caagctacat aacgcactga
     4261 gtcctttagc tgatatactc ttttatgttt ccaagcccaa aattgaggac atataaaata
     4321 tagcacagga atacgcattt catctgccat tttagcaatt cttaaattca aacccgggta
     4381 atcaacgaga atggcaaggt caaatttctc tgtagaaaac agtttgccta atttacgctg
     4441 gactttcagg aaaaagaaca aatgtttcac cacttctaca aaacccatca cagcaaattt
     4501 ttcaaaagga aagagggttt tcaaaccttg cctttgcatt ctgggtccac caattccaat
     4561 atgggttaaa ttaccaaatt tatcgtttaa tgctttcatt accagttcgg aatgcaaatc
     4621 agcagaactt tcacccacaa gccaaaatat tttataactt tctttgcgta tcataaagct
     4681 aaagttccca tcaaagataa agcagttaca ataatagctg ccataattac ccctaaaaat
     4741 ataaaaagca ttgaacgcca aaatcttaag ccaaatatat tagcagctaa agcacctgtc
     4801 caacccccgg ttccgggaag aggaatgcca acaaaaacaa ctaaaccaat ttcctcgtat
     4861 ttttctatgg acttaccttt gcgtcttgtt ctggtaaaaa gccattcagt aaatttacga
     4921 cctggtttca ctttacttat taagcccaaa aaccaatcaa tcagcaacaa taggaaaggg
     4981 actggaatca tattgccaat aatggagaga actgccgctt ccaaccaggg aatattaaat
     5041 aataaaattg ccacaggtat acttccccgc agttctacaa ctggaatcat tgaaattatc
     5101 accacaatga gttcgggtgc aacacctttt gctttcaacc aggcaacggt cttatctgca
     5161 gtagtattac aaaaaagcgg ctgggacaaa attactatca acagaataag cagtatcttt
     5221 tttagctggt tatccaactt cacttccttt ataacattat cataattcca tttatcaaaa
     5281 acccgtattt tgtcaataat ttgtatagac ggatggttga tgttctcgtc aaccaatgat
     5341 tagaaaacga atgaggacat agctcttccg atattaaagt caggagttat atatgctttg
     5401 atatttttta tttgggaaat agcttttaca gcgtatatga ctaatgacga cacagttgct
     5461 cattattaaa atattctgtt catttcggaa ttattagata aaatatctgt gagagattgc
     5521 tgtcacgaat cctcattatt aaaataatta gggtattgtg taaaagttat ttctgcagta
     5581 acgattcctg aaagctcggg taaagatagc tctcaataca tatattttaa tcttttgtta
     5641 cttcaacctt aaatttattc atttttcact tgacaatccc tcaataatat ttatattatc
     5701 tttaagaata tttaaggaga tgaagatgaa tttaaccaac tgtcccattt gtaaaggaga
     5761 attgaaaatt agggtattgc actgcccgca ttgtgaaatt agttttcaag gcgactttga
     5821 aaataactgg ctggcagaat tagacgccgg tcaattagag tttattcgtt tatttttatt
     5881 agtgcaaggt aatttgaaag agctgcaaaa tcagctggga atatcatatc ccacagtaaa
     5941 aaaccgttta gcagaaatta tcgggatcat aacaaaaaaa gaaccggtta cggatacggt
     6001 tattgatgtt ttatccgatt tggaagaggg ttttatcaat gtagacgagg caataaatat
     6061 gattactcaa aggagagaaa aatgagcaaa atcaatttgg agaaaaaatg ggtttttgaa
     6121 cccttacaca aagtagtaat tgaaaatcat ctggctaaat tattatttca agcagatgaa
     6181 ggacaagaag ttaatatgga agggataatc aatttcagtc attctcaaga taccttccaa
     6241 gccgaggatt atattcaatc cgaatatcag gacgggattt taaagattat cctacaagat
     6301 attgattctg atgaagttaa agatgccgtg tttactgtaa ccattccaga aggcgtttat
     6361 ctgaaagtaa aaacagataa ctatcctatc agtttgaata acctgaaaaa caagcttaaa
     6421 gttttgaatg aaaatagtcc catctatctg caaaactgtc aaggcgatat gcatttggaa
     6481 aatgaaaacg gcttgattcg gttgtctgat tgtgaaggta atattgatgc taaattggag
     6541 aacggtccct tatcagcatc caaaattagc ggtcaaaccc ttcatctgga aaatgaaaac
     6601 ggaccgataa aagtgcggat ggcttctttt accgaagtgg aattatattc cgaaaacgga
     6661 cctatcttct acgaaaccat ccctgtagaa aatggcaatt tccagtttaa aaccgaaaat
     6721 ggctcaatta atcttgtgct gcccaataat tttgatttta ctttggaagc aactacacaa
     6781 tggggcagag tgaaaacctc atttgattta ccgattacct ttaatgacaa tatctataca
     6841 atgattaacg gagaaggaac ttcgcaaatt aaggcaattt ccgataatgg aaccattaaa
     6901 atcaatgcag aaaatcgcct gaatttggac tttgtgatga ataaattgga acagattaaa
     6961 atcgccttgc agaaagtaaa ttcggaagca gaaaaacaga aagttgtgga aatggtaaat
     7021 aaaattacca cctatattaa ccgccttgcc gattccatca aagaagaaaa aataaaagag
     7081 aaaataacct ctgctaccag caaattaaag gatttggtag tgaactttga ttttcgtgaa
     7141 actaacgata aggtaattaa aagcgtagaa gatatcggtt cccagattca agatgccttt
     7201 aaagaaggta ttaaaaatat caaagaaagt gtggatgacc tcaaaaaaca tcgcttccat
     7261 accgaatcag tagctgctta tgttaaaaaa attctggatt caccccaaat taaaccttat
     7321 ttgggaggag aacacaagaa aaaagaaaaa gagaatattg cagaccgcag ccgcataaaa
     7381 atcttggaaa tgctggaagc aggaaaaatc actgctgaag aagctgaacg actgttgaaa
     7441 gctattggga aagagtgaaa aaa
//
LOCUS       DAOOZV010000075         7422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_9008_length_7422_cov_58.927107, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000075 DAOOZV010000000
VERSION     DAOOZV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_7422_cov_58.927107"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..824)
                     /locus_tag="PLW08_06910"
     CDS             complement(27..824)
                     /locus_tag="PLW08_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL52366.1"
                     /translation="MNKKSNRQALRHIIQLLFLLLSLAFFAGIIFFSLPYTIHTICPY
                     AIVCFGLSKNILIQYLGNPFAITIAFSFLILVLTIFGGRLFCAYICPLGTLQEIIYAL
                     FGKKRKDRKQIPQFQERRFASVKYIVLVVTIILSLIGVNYIFIHLCPVYALSLLPDIA
                     VWGLIVTLLIIVAGIFLERFWCRFLCPYAALMNLFQHLGQLVHFPRLKIHRNLERCND
                     CELCSLSCPMNINITENESVQNPNCILCFKCREKCPKPETLKGKMEK"
     gene            complement(945..3671)
                     /locus_tag="PLW08_06915"
     CDS             complement(945..3671)
                     /locus_tag="PLW08_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPL52367.1"
                     /translation="MDAKEIRKKAQLQKAREAQKTPPAYQKPVPRPLFEDETMRPQNI
                     VPVKLNRWIAFGVFLVVLIVYMCTQARTMSFWDSGEYATCISILGVPHPPGNPFYILF
                     GRAIVAIFGGIFPHAMIAAFISALFSALAVMFTYLITIQLTSMLKVKPWEAIFAGIIA
                     SFYTAFSFTFWMNAIEAEVYSGLVFFVNLIFWLTLIWVQKSRDFSHQNILLLIAYLFF
                     LGFCVHQTALQIAPAMLFIVCYPLLRQGIKSGSFFPKLVVYSIFLLVGYFLFGVIGKQ
                     VQIDDLDKMGFALVAFIIIFIELRKVLDRRIWLLGIALVLVGLSSHIYLMVRAAERPF
                     INEGNPSNWKMFQEYVLRKQYGNTSFVQRRGNFFKDQLNYHFLRYFGMQWLNEPFFTK
                     PLGVTSALLKPIANIFIAFLGIVGAMLQFRKNKHSFWYFFSVILITTVVMVFVMNLSD
                     AEVRDRDYFFVVAYNMWAIWMGIGALALVTLWKSKPVRVVLLIFMCLLPVSNLISQYR
                     VHDRSQEFIALDYGLNFLNSVEENAIIFTNGDNDTFPLWYAQAVEDPYSKENIYPARD
                     VYPTKESKEAIAKAMEYKNKCLKGIRKDVSVANLSLLNTSWYIRQLRDQEGILFNIPD
                     DQLDELEPRRIEKPLVIPGPPDNPSMGFTMDIPPTAEWRTKEPFYRVSDLAVMQIVKD
                     NYGKRPIYFAVTCESYVNFDDYVRNEGMVGRIVSTPKEGQIDPERLLHNIDVVYNYRS
                     IEDPRVYKDDNMRRLVMNYGSGFVRAANYFVDTENYPKALEYINKAKKFVDDEIKLTE
                     FYTNYYSRTGQWNKLDEFINRNLATHPDGWKIYLSYIIMHLVDYYPDKIITYLKKGFL
                     LFPDQEYFAQFAVSYAQEYKEYDVIHKLLEEVAPKLQYDISRYQSDLEMLSE"
     gene            complement(3684..4670)
                     /locus_tag="PLW08_06920"
     CDS             complement(3684..4670)
                     /locus_tag="PLW08_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPL52368.1"
                     /translation="MPEKIRMTIIYTVLTICGLAMIIPFIWMLSTSLMTQSEFNKQES
                     TFIPKESYYVWDNGTQKQKIILVQEKGNTSIIHILNEEDKIIEEYKEVPSSQIKMIKK
                     VPSFHWQNYVKAFNKVPFIIYFKNTLIVSFLTLIGVLITSTLAAYAFARMEFLGRDFL
                     FYLFLSMMMVPEPIYLVSSYVLLDKISWLDTYQALIVPWCVNIFTIFLFRQHFKSLPQ
                     ELFDAAAIDGCSTFGMLWRIMLPLSKSVIATASIFSLIGSWNSFMWPLVMTNRPELRV
                     LQVGLSYFNQEASTQTTLLMAASTFSIVPILILFFMAQKQIIASYAKAGLKD"
     gene            complement(4686..5618)
                     /locus_tag="PLW08_06925"
     CDS             complement(4686..5618)
                     /locus_tag="PLW08_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPL52369.1"
                     /translation="MKTKRKISPYLYILPALFLLVAFRLIPIIMSFVISFFDWSITGT
                     GKFIGLLNYANMLKDSVFWQSMGNTFWLVIFVVPLSIIFSLLFAVLLNNIKAVKGLFR
                     TVYFMPYVTSLVAVSIVWKLMFNEQTGLINTLIGYIGIPPQKWLSESRGIFELLFAKA
                     GTSFPRFLGGPSQALFAIIIMTVWKGLGYNTIIYLSGLQNISKVYYEAAEIDGASKIK
                     QFFKITLPLVSPTTFYVLIMTTIVTFQTFSQVYLMTDKGGPLNTTKLIVYYIYEKGFD
                     TLEMGYASAVALVLFIIILALTIYQRRLEKYVNY"
     gene            complement(5722..6933)
                     /locus_tag="PLW08_06930"
     CDS             complement(5722..6933)
                     /locus_tag="PLW08_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPL52370.1"
                     /translation="MELSKRAREIQASPIRKLNPYANSAKKRGIKVYHLNIGQPDLPT
                     PKEMLKAYHEFSDEVLEYGPSQGLDIYREGLVKYYGNLGIELKVDDIIVTTGGSEAIT
                     FAMMVVCEVGDEIIVPEPFYTNYNGFATMAGITLIPLTTYAENGFALPGNSVIEAKIS
                     FKTKAIMLCNPGNPTGTVYSREEIFRIADLAKRKGLYFISDEVYREFIYDGLSHTSVL
                     EVPDFEENAILVDSVSKRYSACGARIGCIVSKNKEIMSSVLKFAQARLCPPTVDQLVA
                     NAGIYLPETYFEQLKVEYQSRRDLVLSELEKIPGVICKKPEGAFYVFAKLPIDDAEDF
                     IIWMLKEFQINNETVMAAPGDGFYATPGLGKNELRLAYILCKEDLKKAMYIFQNGLAE
                     YQKLHIGKASS"
BASE COUNT         2439 a         1433 c         1321 g         2229 t
ORIGIN      
        1 accaatattt gcagaataac tttcatctat ttttccattt ttccttttag agtttcgggc
       61 ttagggcatt tttccctgca cttgaaacaa agaatgcaat tcgggttctg cacagattca
      121 ttttccgtga tattaatatt catcggacaa gatagagagc aaagttcgca atcattacag
      181 cgttccagat tacggtgaat ttttaagcgg ggaaaatgga caagttgtcc caggtgctga
      241 aacaaattca ttaaagcagc gtaaggacaa agaaatctac accagaagcg ttccagaaaa
      301 attcccgcca caattatcaa gagagttaca atcaaacccc aaacagctat atcaggcaaa
      361 agggaaagag cataaacagg gcataaatgg ataaaaatat agtttactcc tattagagaa
      421 aggatgatag ttacaaccaa cactatatat ttaacagaag caaagcgcct ttcttgaaat
      481 tgagggattt gttttctgtc ttttcgcttt ttgccgaaaa gggcatagat aatttcctgc
      541 aaagttccca gagggcaaat ataggcacag aataggcgac caccaaaaat cgttaaaacc
      601 agaattaaaa agctgaaggc aatcgttatc gcgaaagggt tgccaagata ttgaataagg
      661 atgtttttgc ttaagccgaa acaaactatg gcataaggac aaatagtatg aatagtgtaa
      721 ggtaaactaa agaaaatgat gcctgcaaaa aaagccagtg ataacaataa aaagagcagt
      781 tggataatat gcctgagggc ttgtctgttg ctttttttat tcattttgga tctttcttgg
      841 tcgtcattag tcctcactgc caaaatagtt ttcagatagg gttaaagtta tttcggcagt
      901 gacgacttct gacttaattc attctacata tattcactta ttttttattc agagagcatt
      961 tccaggtctg attgatatct gcttatatca tattgaagtt taggagctac ttcctccaga
     1021 agtttatgaa ttacatcata ttctttatat tcttgagcat aactgacagc aaattgggca
     1081 aaatattcct gatcagggaa aagtaggaac ccttttttga ggtaggtgat gattttatcg
     1141 ggataataat ctaccaaatg cataataata tagcttaaat aaatcttcca gccatcggga
     1201 tgagtagcga gattgcgatt gataaattca tccagtttgt tccactgtcc tgttctgctg
     1261 taatagtttg tatagaattc ggtcagtttt atttcatcat ccacaaattt ctttgcttta
     1321 ttaatatatt ccaaggcctt aggataattt tccgtgtcca caaaatagtt ggcagcacgc
     1381 acaaaaccgg aaccgtaatt cattacgagt cgtctcatat tgtcatcttt atataccctt
     1441 gggtcttcta tagagcggta gttataaacc acatcaatat tatgtaacaa gcgttccggg
     1501 tctatttgtc cttctttggg agtggaaaca attctaccaa ccataccttc attacgaaca
     1561 taatcatcaa aattaacata gctttcgcag gttacggcaa aataaatagg tcgtttgcca
     1621 taattatctt tcacaatttg catcacggca aggtcggaaa cacgatagaa aggttctttt
     1681 gttctccatt cggcagtagg aggtatatcc attgtaaaac ccattgaagg attatccggt
     1741 ggtcccggaa taaccaacgg tttttcaatt cttcttggtt ccaattcatc taactggtca
     1801 tcaggaatat taaacagaat tccttcttgg tctctaagtt gtctgatata ccaacttgtg
     1861 tttaaaaggg aaagattggc aacggaaaca tctttccgga tacccttcag gcatttattt
     1921 ttatattcca ttgccttagc gattgcttct ttactttcct ttgtaggata aacatctctg
     1981 gcaggataga tattctcttt ggaataaggg tcttcaaccg cttgtgcata ccagagagga
     2041 aaagtgtcat tatctccatt ggtgaaaatg atggcatttt cttccaccga attaaggaaa
     2101 ttcaatccat aatccagagc gataaattcc tgagaacgat cgtgaaccct atattgagag
     2161 atcaaattgc taacgggcaa aaggcacata aaaattaata aaacaactct tacaggttta
     2221 cttttccata gtgtaactaa tgccaaagct cctattccca tccagattgc ccacatatta
     2281 taggcaacta caaaaaagta atcacgatca cgaacttcgg catcggaaag attcattaca
     2341 aaaaccatta ctactgtagt aattaagatt accgagaaga aataccaaaa actgtgttta
     2401 tttttgcgaa attgtaacat agcgccgact attcccaaaa aggcaataaa aatattagct
     2461 atgggtttca acagagcgga agttacacct aagggttttg tgaaaaatgg ctcgtttaac
     2521 cattgcattc caaaatagcg taaaaagtga taatttaact ggtctttaaa gaaatttccc
     2581 cgcctttgca caaaactcgt attaccgtat tgttttctta aaacatattc ctgaaacatt
     2641 ttccagttgc tgggattgcc ttcattgatg aaaggtcttt ctgcagctcg caccattaaa
     2701 taaatatggg aagaaagacc aactaaaact aaagcaatac ctaaaagcca aatccggcgg
     2761 tcaagcactt ttcttaattc tataaatata ataatgaatg cgacaagagc aaaacccatt
     2821 ttgtccaaat catctatctg cacctgttta cctatcacac cgaagaggaa ataacccacc
     2881 agtaaaaaaa tagaatagac aacgagtttt gggaaaaaac tgccactttt aattccttgt
     2941 cttaatagag gatagcaaac aataaacagc attgcgggtg ctatttgtaa agctgtttga
     3001 tgaacacaga aacccaggaa aaagagataa gcaattaaga gcagaatatt ctggtgagaa
     3061 aaatctcggc ttttttgcac ccagattaaa gtgagccaga agattaaatt cacaaaaaag
     3121 accaatccgg aatagacctc tgcctcaata gcattcatcc agaaagtgaa agaaaaagca
     3181 gtgtaaaatg aagcaataat tccggcaaaa attgcttccc aaggtttaac tttcagcata
     3241 ctggtaagct ggatagttat caagtaagta aacattactg ccagagcgct aaagagagca
     3301 gaaatgaagg cagcaatcat tgcatgcggg aaaatacccc caaaaatggc aactattgct
     3361 ctgccaaaca agatgtaaaa agggtttcca gggggatggg gaactcctaa aatgctaatg
     3421 caggttgcat attcaccgct atcccaaaag ctcatagtgc gggcttgagt acacatataa
     3481 acaattaaaa cgacaagaaa gaccccgaaa gctatccaac ggttcaattt taccggcaca
     3541 atattttggg ggcgcattgt ttcatcctca aaaagaggac gaggaaccgg tttttgatag
     3601 gcaggaggtg ttttttgtgc ctctctggct ttctgtaatt gtgctttttt tcttatttct
     3661 ttggcatcca cttttttctc cttttaatcc tttaatcctg ctttagcata gctggctata
     3721 atttgttttt gtgccataaa gaacagaatc aatatgggga caatgctaaa ggttgaagca
     3781 gccattaaaa gtgttgtttg cgttgatgct tcctgattaa agtagctaag accaacttgc
     3841 aagaccctta attcaggacg gttagtcatt accagtggcc acataaaact gttccagctt
     3901 ccgattaaag aaaaaatgga agcggtagca ataaccgatt tggaaagagg aagcataatt
     3961 ctccagagca ttccaaaagt actgcagccg tcaatagcag cagcatcaaa aagttcctgg
     4021 gggagtgatt tgaaatgctg acggaagagg aaaatagtaa aaatgtttac acaccaaggg
     4081 actataagtg cctgataggt atctagccaa ctgattttat ccagcaaaac ataggaagaa
     4141 accagataga tgggttccgg aaccatcatc atacttaaaa atagatagaa taaaaagtcc
     4201 cttcccaaaa attccattcg ggcaaaagca taagcagcaa gggtagaagt aatcagcact
     4261 ccgattaaag ttaagaaact aactatcagt gtatttttaa agtagataat aaaaggaact
     4321 ttgttaaatg ccttaacata attctgccag tgaaaactgg gaactttctt aatcattttt
     4381 atttgggaac tggggacttc cttatattcc tcaattattt tatcttcttc attcaagata
     4441 tgaatgatgc tggtattgcc tttttcctgc accagaataa ttttttgctt ttgagttcca
     4501 ttgtcccaaa cataatatga ttccttggga ataaaagtgc tttcctgttt attgaattcg
     4561 gattgtgtca ttaaagaagt ggaaagcatc cagatgaaag ggataatcat agccagtcca
     4621 caaatagtaa gaactgtgta tatgatggtc atacgaatct tttccggcat ttccttcctc
     4681 cttccttaat aattaacata tttttctaac cgtctttgat agatggttaa tgcaaggata
     4741 atgatgaaaa gaactaaggc aacagcactg gcataaccca tttccaaagt gtcaaatccc
     4801 ttttcataaa tataatatac aatcagtttg gttgtattta gtggtccgcc tttatccgtc
     4861 attaaataga cctgggaaaa ggtctggaaa gtaacaatcg ttgtcataat cagcacataa
     4921 aaagtagtag gggagaccag aggcagagtt attttgaaaa attgtttgat tttactggcg
     4981 ccatcaattt cggctgcctc ataatagacc ttggaaatgt tttgcaaacc agaaaggtaa
     5041 ataattgtat tataacccaa gcccttccaa acagtcataa taataattgc aaaaagcgct
     5101 tgggaaggtc cccccaaaaa gcggggaaaa gatgttcctg ccttggcaaa cagcaattca
     5161 aatatacctc tggattcgga taaccatttt tgaggtggaa ttccaatata accaattaaa
     5221 gtatttatta agccggtttg ctcattgaac atcagtttcc aaacaattga aactgctacc
     5281 agggaagtta catagggcat aaaataaacc gtgcgaaaaa gtcctttaac agctttaata
     5341 ttgtttaaca gcaccgcaaa aagcagagaa aaaataatgc ttaagggaac tacaaaaatc
     5401 accagccaga atgtattgcc cattgattgc caaaaaacgc tatctttcaa catattagca
     5461 taattaagca agccgatgaa tttgcctgtg cctgtaattg accagtcaaa aaagctgatt
     5521 acaaagctca ttatgattgg tattaaacga aaagcaacca gcaaaaacaa agccggcaga
     5581 atatagaggt agggagagat ttttcgtttc gttttcatac tattgtcctt tcatttctcc
     5641 tttatcggga tttatcagtt ccctttttgc tttggtatcc tgaaaatccc cttccatttt
     5701 cttcattttt tccctttaat attatgaact tgcttttcct atatgcaatt tctgatattc
     5761 ggcaagtcca ttttgaaaaa tgtacattgc ttttttcagg tcttctttac aaagaatata
     5821 agccaggcga agttcatttt tccctaatcc aggtgtagca taaaatccgt cacccggagc
     5881 cgccataacc gtttcattat taatctgaaa ttccttcagc atccagataa taaagtcctc
     5941 tgcatcgtca ataggtagct tggcaaacac ataaaaagcc ccttccggtt ttttacaaat
     6001 aacaccagga attttttcca gctcacttaa taccaaatca cgccgtgatt gatattctac
     6061 ttttaactgt tcaaaatatg tttccggtaa ataaattccg gcatttgcca ccaattgatc
     6121 cacggttgga gggcataaac gcgcctgagc aaactttaat accgaggaca ttatttcctt
     6181 gtttttgctt acaatacaac caattctggc tccacaagca ctgtagcgtt tggaaacact
     6241 atccactaaa atagcgtttt cttcaaaatc gggaacttca agaacgctgg tatggcttaa
     6301 tccatcataa ataaactccc ggtagacctc gtcagaaatg aaatagagac ctttgcgttt
     6361 tgccaaatca gcaatgcgaa aaatttcttc tctactgtaa acagttcccg ttggatttcc
     6421 cggattgcaa agcattatag ctttggtttt gaaactgatt tttgcttcta ttaccgagtt
     6481 cccaggtaag gcaaaaccat tttctgcata agttgttaaa ggtatcaaag ttatccctgc
     6541 cattgtagca aaaccgttat agtttgtata aaacggttca ggaacaataa tttcgtctcc
     6601 aacttcgcaa acaaccatca tcgcgaaagt tattgcttcg ctaccaccgg ttgttacaat
     6661 aatatcatct acctttaatt caatacctaa atttccgtaa tatttaacta aaccctcacg
     6721 ataaatatcc agaccttggg aaggtccgta ttccaaaacc tcatctgaaa attcgtgata
     6781 tgccttcaac atttctttcg gggtgggcaa atcgggctga ccaatattca gatgataaac
     6841 cttaattccc ctttttttcg cggaattggc ataaggattt aatttacgga tgggtgatgc
     6901 ctgaatttct cgggctcgct tagagagttc cattttttgt ttcctttata ttttatttta
     6961 tattataatt ttcaactgga ctgtaatgta tataggcaac tacaatctcc aacttttttt
     7021 atccggaagg ttgaattcct catcaaccag ctttttcgct ttttcttctt gccgaaaagc
     7081 ttataataat gcaggaactg gcttgtaaga ggaacaacag gatgtcgttc ttccgaaaaa
     7141 atttagccag agattaccgt tccttcgctt ctatatttct tttccgatac ctactattta
     7201 caatcctgaa aaaagacaag aaaaatcttt gtgcatacaa agaaaatatt atctgcttaa
     7261 acataaataa tgcagttgag gaaaaatggg ttggcaaatt ccaaattgct gaaaagttcg
     7321 cagcactctg aaaaataatt gtccataatt tatggtgaat tagaattcag caaaccaaaa
     7381 gcaaaaattg acttttcttc tccgggttga aacccttcta ta
//
LOCUS       DAOOZV010000076         6910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_9785_length_6910_cov_49.721663, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000076 DAOOZV010000000
VERSION     DAOOZV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_6910_cov_49.721663"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLW08_06935"
     CDS             complement(<1..522)
                     /locus_tag="PLW08_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HPL52371.1"
                     /translation="MILNCAEELILLSIDDQTNSFYRITNINFNVALIGALLMDLALR
                     KRIDVDLEGIYVLSTEPTGDKFLDAILENLIETEAGNQPAVLVGQLYNSMGHLKDKLL
                     QDLEEKGIIKVSQCKVLWLFKKRRYPIIDQKEEEEVLSRIRKTVLTDVDPEPRDLALI
                     ALLNICNLMDKIFT"
     gene            complement(544..1833)
                     /locus_tag="PLW08_06940"
     CDS             complement(544..1833)
                     /locus_tag="PLW08_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL52372.1"
                     /translation="MRKFFIMIIMLFVLLAGCKSALQSNKVKVAFWHGLSGPLGDTLN
                     EMIREFNHTHKDIEVIANPISSYTALSQKLMASIQAKKQPDIAQVFESWTSKYIEAGV
                     LCNFEDLMKADSTWTMQSLEDIYPVFLKSNTFNDTIYSFPFNKSVRAYFYNKDAFYRA
                     GLDPNHFPATWDEFRSYCRKLTTDKNNDGKPETYGTNFNVNEWQFINLLYQAGGTILD
                     EKGNPQLNSPQAIEALTFTTDLLNKDKTVYLVREFEGQNDFLAGIAAMYEGSSVSITH
                     MRQQPINFNIGYAPLPVYRTNKSAVSGTNIVIFKSGDRKREQAAWEFIKWFTDTEQTA
                     RWSAETCYMPVRKSAMQSPVLQNFLKEYPQFQGIYDQLETAIFEPQISAWFKARMELK
                     NYLEKAMRGISTPQEALDEMNAKFQAIIEEEKYLKSL"
     gene            complement(2157..2870)
                     /locus_tag="PLW08_06945"
     CDS             complement(2157..2870)
                     /locus_tag="PLW08_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPL52373.1"
                     /translation="MLKFTPDTNVLVLTGAGISAESGIRTFRDSCGLWENHRVEDVAT
                     PEAFNSNPVKVWEFYRQRYQDSISSKPNPAHFALVKLEDFLGDNFLLVTQNVDCLHIK
                     AGSKRVLEMHGSLANCICTKCHSKLAIKDIDLTQPIPLCPFCNGILRPDIVWFGEIPH
                     YLSIIDDALRKCSVFIIVGTSGVVYPAAGFVLTAKLMGAKTIAINLEKPDNLAYIDEF
                     YQGKSGEILPGLVAMWIGE"
     gene            complement(2873..6391)
                     /locus_tag="PLW08_06950"
     CDS             complement(2873..6391)
                     /locus_tag="PLW08_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL52374.1"
                     /translation="MNYSLETPKIEAKEQYTKVSLEKAQSWGQPGNPDLPWLGIKLLL
                     PAGFEAEKIVVNRYNPVTYTLDKPIAPIQRQYPFSHIILETPDKPNAEVYDSPLPYPQ
                     NTDNGLITSFMCGQPIAFSAISPFSYYPLNNELIFYRQINVEIIYSSSAKAMEAMRFL
                     KQDSFTANRLQKIVDNPETITISSTRETGIEYLIVADAEKITQWTPLKTFQETRGKTV
                     LIKSMSEIIAENQGVDTQDKLRNYIISLYTTNPLLYVFLAGDTDVIPHRGFYVNVNNS
                     NEGTDADIPADMYYACLDGNWNTDGDSYWGEMYETDLTPELAIGRFCYNNDAEIANFL
                     NKLTNYSNNPVANEVKSAFFVGEWLWDGPTWGGDYMDEMIGGSSNNGYTTVGVPTSWN
                     ITTLYDRTYGAADAWGANQIRPMLSQGPNLVNHLGHSNTTYAMRLSNNQVSASTITND
                     GVNHNFSTYFTQGCYAGAFDNRETSPGQYTSDCITEKMTSIATGPVAMISHSRYGWGV
                     QQSTDGASQYIHRQFIDAIFGENIYEAGFALVDSKIDNIPYITNEPVMYWVTYETNFF
                     GDPAMMVWTDTPQTITANLPEVLMVGQNHYQIQTNAPYAVFILKNEDHTVYRTSADEN
                     GLIIVNFFAPLTPGTYYIYISAHNFYPFNATITATAAQMPYIICNNVSYNDQDGLYHT
                     GETLSLNFFVKNVGLMDLTVPGTLTFLSNSANIQILSSTANFTGIAAGDSLALNSVFL
                     FRIVGNFTDQTQATFTIRANYDSYLSNSTFILTLNAPVLQLLSYQLNNMDVMIMPGDT
                     PSISFSVANTGSGNAYTPMLIIFNSSPDIILEQDEILLAPLSPHSTADYQSAITLQVL
                     DSAVIDNNYMINYIFSAENGNLVEGNFLIHIGALSYSFETDYQTWETVALSSNFVNQW
                     HRSSNRNHTTNGIYSMKFGGNGSSNYASSAYGALISPVMNVSPNCQLKFYHWMSAEKH
                     STNQSYAWDGGLVQMSLNGGNWTQITPVGGYPYKIYNNSASPFSANTWVYSGNFDWTE
                     AIFELGNVSGIAQFRWVFGSDGYVGGEGWYIDDVRITGYVANEDESIPLAQNVILYDN
                     YPNPFNPETTIEFSIPARMPVCLEVFNIKGQLVTRLIDEVLPAGTHKVVFNGLDINKR
                     NIASGVYYYRIKTPSGVQMRKMLLLK"
BASE COUNT         2169 a         1331 c         1220 g         2190 t
ORIGIN      
        1 agtaaagatt ttgtccatca gattgcaaat attaagtaaa gcaatcaaag ccaggtctcg
       61 gggttcagga tcaacatctg taagaaccgt tttccgaatt cgggaaagga cttcttcctc
      121 ttctttctgg tctataatgg gatagcgacg ctttttgaac agccaaagga ctttacactg
      181 gctcaccttg atgatgcctt tttcttccaa gtcctgcagg agtttatctt tcaagtgtcc
      241 catagagtta taaagctgtc ctactaaaac agccggttgg tttcctgctt cagtttcaat
      301 taaattttcc aggatagcat ccagaaactt atcacctgtc ggttctgtag atagaacata
      361 gataccttcc agatctacat caattcgttt tctcaaagcc aagtccatta aaagagcacc
      421 aattaaagcc acattgaaat tgatgttggt tatcctgtaa aatgaattag tttgatcgtc
      481 tatggatagc aggattagtt cttcagcgca gttaagaatc atattttcct cgttggttaa
      541 tagttacaag ctttttagat atttttcttc ctcaataatg gcttgaaact ttgcgttcat
      601 ttcatccaat gcttcttggg gagtagaaat tccccgcatt gctttctcta aataattttt
      661 taattccata cgggctttga accaggcaga aatttggggt tcaaagatag ctgtttccag
      721 ctgatcataa ataccttgaa attgaggata ttcttttaaa aagttctgca gaacaggaga
      781 ttgcatagca cttttccgca caggcatata acaggtttca gcactccatc tggctgtttg
      841 ttctgtatct gtaaaccatt ttataaattc ccaggcagct tgttctcttt ttctatcacc
      901 gcttttaaaa ataacaatat tagttccact gacggcactt ttattggttc tatatacagg
      961 taaaggagca taaccaatat taaaattgat gggttgctga cgcatatgcg tgatagaaac
     1021 actactacct tcatacatag cggcaattcc ggctaaaaaa tcattttgtc cttcaaattc
     1081 acggacaaga taaacggttt tatctttatt tagcaaatcg gtagtaaaag ttaaagcttc
     1141 aattgcttgt ggactattaa gttgtgggtt tcctttttca tctaaaattg ttcccccagc
     1201 ttggtaaagc agattaataa attgccattc attcacatta aaattggttc cgtaggtttc
     1261 tggtttaccg tcattgtttt tatcagtagt taatttacga cagtaggaac gaaattcatc
     1321 ccaagtagca ggaaaatgat tagggtcaag accagctcta tagaaagcat ctttattata
     1381 gaaataagca cggacacttt tattgaaggg aaaggaatag atggtgtcat taaaagtatt
     1441 ggattttaag aagacaggat aaatatcttc caaactttgc attgtccagg ttgaatccgc
     1501 tttcattaaa tcctcaaaat tgcaaagcac tcctgcctct atatatttgg aagtccaaga
     1561 ttcaaaaacc tgagcaatat cgggctgttt ttttgcttga atggaagcca taagtttttg
     1621 cgagagcgcc gtatagctac taataggatt ggcaattact tctatgtctt tatgagtgtg
     1681 attaaattca cgaatcattt cattaagagt atcacctaaa ggaccgctga gaccatgcca
     1741 gaaagcaact ttaaccttat tggactgtaa agcactttta cagccggcta aaaggacaaa
     1801 gagcattatt atcattataa agaacttacg cattatttac aactccttat ttgataaaag
     1861 cgattcaact accatcttta tttgttcagg gttagcgtca acgaaaaaaa tgaaattgaa
     1921 aaagaaaaca cagagaaaag aaattagttt tacaatataa gcgtaatctg gaagagtcgt
     1981 tattacgcaa gaaaaatgta aaaaaagtga gaacggaaaa attatttatt ccggaagtta
     2041 tctcactgct ttagccctca ctttttttaa tctcactttt tcattcttca tttcctaccc
     2101 ctgcttaatt atcaattttt tatctctgaa atctgtgtaa tctgtgagca ataatcctat
     2161 tcgccaatcc acatagctac taatccgggc agaatttctc ctgatttgcc ctgataaaat
     2221 tcatcaatat aggcaaggtt atccggtttt tccagattta ttgcaattgt ttttgctccc
     2281 atcagtttgg cagtaagaac gaaacctgct gcaggataaa caactccgct ggttcctaca
     2341 atgataaata cagaacattt tctgagagca tcatcaatta tactcagata atgcggtatt
     2401 tcaccaaacc agacaatatc tggtcttaaa atcccattgc aaaagggaca aaggggaatc
     2461 ggttgagtta aatcaatatc cttaattgct aatttgctat ggcatttggt acaaatacaa
     2521 ttggcaaggg agccatgcat ttccaaaact ctcttagagc cagcttttat atgcaagcaa
     2581 tccacatttt gggtaaccag taaaaagtta tcccctaaaa aatcttccaa tttaaccaaa
     2641 gcaaagtgag ccggattggg tttactggaa atactatcct ggtagcgttg acggtaaaat
     2701 tcccaaactt taaccggatt agagttaaaa gcttcaggag tagcaacatc ttcaacccga
     2761 tgattttccc ataaaccaca gctatctcta aaagtccgga taccggattc agcgctgatt
     2821 ccggctccgg ttaaaacgag aacatttgta tcgggagtaa attttagcat ttctatttca
     2881 gtaatagcat ttttcgcatt tggacacctg aaggagtttt aatgcgataa taatatactc
     2941 cactggctat attacgcttg tttatatcta aaccattgaa tacgacttta tgagtgcctg
     3001 caggtaaaac ctcatcaatt aagcgggtta ccaattgacc tttaatattg aagacctcca
     3061 gacaaaccgg catccgagct ggtatggaaa actcaatcgt agtttcagga ttaaaaggat
     3121 tgggataatt atcatacagg ataacatttt gtgctaaagg aatgctttca tcctcattgg
     3181 caacatagcc cgttatcctc acatcatcta tataccatcc ctctccacca acataaccat
     3241 cactgccaaa aacccaacga aattgagcta ttccggaaac attaccaagt tcaaaaatag
     3301 cttccgtcca atcaaaatta cctgaataga cccaagtatt tgcggaaaaa ggtgaagcgg
     3361 aattattgta aattttataa ggataaccgc ctacaggggt aatttgagtc caattgcctc
     3421 cgttcaaact catttgcacc aaaccaccat cccaagcgta ggattgattg gtactatgtt
     3481 tttctgcgct catccagtga tagaatttca attgacaatt ggggctaaca ttcattacgg
     3541 gactaataag tgcaccataa gcactactgg cataattaga agaaccgtta cctccaaatt
     3601 tcattgaata aataccgttg gtagtatgat tacgatttga tgaacgatgc cactgattta
     3661 caaaattact gctgagagca acggtttccc atgtttggta atcggtttca aaactgtaac
     3721 tgagagcacc aatatgaatt aagaaatttc cttctaccag atttccgttt tcagcactga
     3781 aaatgtaatt tatcatatag ttattatcaa taaccgcact atccaatacc tgtaaagtga
     3841 ttgcactttg gtagtcggct gtgctatgag gacttaaggg agccagcaaa atttcatcct
     3901 gctccaaaat aatatcggga gaggagttaa aaataattaa cataggtgta taagcatttc
     3961 cactaccggt attagcaacg ctgaaactaa tggaaggtgt atctccaggc ataatcataa
     4021 catccatatt attcagctga taagaaagca attgtaaaac cggagcattc agagttaaga
     4081 taaaggtaga attggataag taggaatcat aattggctct gattgtgaag gtagcttggg
     4141 tttgatcagt aaaattgcct acaatcctga acaaaaagac agaattaaga gctaaagaat
     4201 caccagcagc aataccggta aaatttgccg ttgaggaaag aatttggata ttagctgaat
     4261 tactgaggaa agttagagtc ccgggaacag tgagatccat caagccaaca tttttaacaa
     4321 agaaattaag ggacaaagtt tcacctgtat gatagagtcc atcttgatcg ttataactga
     4381 cattattaca tattatatag ggcatttgag ccgctgtagc tgtaatggta gcattgaaag
     4441 gataaaaatt gtgggcactg atataaatgt aataagttcc tggagttagc ggagcgaaaa
     4501 aattaactat aatcaaacca ttttcatctg cggaagtcct gtatacggta tgatcttcat
     4561 ttttcagaat aaataccgca taaggtgcat tggtctgaat ctgataatga ttttgtccta
     4621 ccattaatac ttccggtaaa ttagctgtta tagtttgtgg agtgtccgtc caaaccatca
     4681 tagcaggatc accaaaaaaa ttggtttcat aagttaccca atacattacc ggttcattag
     4741 tgatataggg aatattatct attttagaat caaccaaggc aaaacccgct tcataaatgt
     4801 tttccccaaa aatagcatct ataaactggc gatgaatata ttgagaagct ccatcagtac
     4861 tttgttgaac accccagcca tagcgtgaat gagaaatcat tgctacaggt cctgtggcaa
     4921 tagaagtcat tttttctgtt atacaatcgg atgtatactg cccgggagag gtttcacgat
     4981 tatcaaaagc accggcataa cagccctggg taaaataagt ggaaaaatta tgatttacac
     5041 cgtcattagt aattgtggaa gcactaacct gattatttga taaccgcatt gcataagtag
     5101 tgttggaatg tcccaaatga tttaccagat taggaccctg actaagcata gggcgaatct
     5161 gattagcacc ccaggcatca gctgctccat aagttctatc atataatgta gttatgttcc
     5221 aggaagtagg aactccaact gtagtatagc cattattact acttccacca atcatttcat
     5281 ccatataatc accaccccag gtaggtccgt cccataacca ttcaccaaca aaaaaagctg
     5341 atttaacctc atttgccacc gggttgttgc tgtaattggt aagtttattt aggaaatttg
     5401 ctatttctgc atcgttatta tagcaaaaac gcccgattgc cagttccgga gtaaggtctg
     5461 tttcatacat ttcaccccag taactatctc cgtcagtatt ccagtttcca tccaaacagg
     5521 cataatacat atcagcagga atatctgcat ccgtgccttc attgctgtta tttacattaa
     5581 cataaaaacc tctatgagga ataacatccg tatctcctgc cagaaaaaca tagagcagag
     5641 gattagtagt atataaggaa ataatgtaat tacgtaattt atcctgggtg tcaactccct
     5701 gattttccgc gataatttca ctcatacttt ttattagcac agtttttccg cgggtttctt
     5761 ggaaggtttt tagaggtgtc cactgggtta ttttttccgc atctgctaca ataagatatt
     5821 caattcccgt ttcacgagtg gaggaaatag tgatggtttc agggttatcc acaatttttt
     5881 gcaaacggtt tgcggtaaaa gaatcctgtt ttaagaaacg cattgcttcc attgctttag
     5941 cactgctgct gtaaataatt tctacattta tttgtctata aaatatcaat tcgttgttta
     6001 aaggataata ggaaaatgga cttattgcag aaaaagctat cggctgtccg cacataaaag
     6061 aagtaatcaa tccgttatcc gtattttgag gataaggaag aggcgaatca taaacttccg
     6121 cattgggctt atccggtgtt tccaatatta tatgggaaaa gggatattgc ctttgaatcg
     6181 gagctatggg tttatctaaa gtgtaagtaa caggattata tctgttaact acaatttttt
     6241 ctgcttcaaa acctgccggt aataaaagtt ttatccctaa ccagggcaaa tctggatttc
     6301 caggttgacc ccaactctga gctttttcca aacttacttt cgtgtattgt tcttttgctt
     6361 caattttcgg tgtctccaag gagtaattca tcgttactcc agctataaga accgctgttg
     6421 aaaacaagag tatcgccaat aatgctatgt ttttcatagc ttcctacctt tctgctgtaa
     6481 tttttttctc tcttatttta tgcaatgctt gttccatatt ctgaaaataa ttcatagttc
     6541 tttaactttt ggtttattat tataaaatac agagggcaga aaacattttt aattgctttc
     6601 gtatttaata atatccctat tttattatcc cttcttttcc gaagcaaata actatgttaa
     6661 attacgctcc cttgctacta tttatccgct tttagtggct ttttagcgat tatttttttc
     6721 cttacttaaa gttagttcct ttaggaatag aagttcccta acaatgtttc tcaaccttgt
     6781 tatagaattc attgcaattt atatagttac aagtgcttcc ttactaccca cttatcatcc
     6841 tctaatcatt cactcaacct caacccatac aaagcttggg ttgtgtatcg gagaatataa
     6901 agtggtattt
//
LOCUS       DAOOZV010000077         6694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_10163_length_6694_cov_58.002109, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000077 DAOOZV010000000
VERSION     DAOOZV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_6694_cov_58.002109"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..848
                     /locus_tag="PLW08_06955"
     CDS             342..848
                     /locus_tag="PLW08_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52375.1"
                     /translation="MKIARHFFCELKRNREPAPQLCAVCPRILKCKSFYAWHKLHHQE
                     YLNFVIEITKKFPDKYTMEVHFMPEKQTFVQIVDMATGKIEKVVSLTEIEALSAEDKL
                     ALSRNKNLFIVTHRLEPIVKIELKKTVISAPIQFMESAPKEEEPIEEVTPLPINPEKP
                     KPKRTPKN"
     gene            1137..1502
                     /locus_tag="PLW08_06960"
     CDS             1137..1502
                     /locus_tag="PLW08_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52376.1"
                     /translation="MFILDEIHLDVAKAIAEKNRKKKSCDHCYDRGWIGVSEQNLLVL
                     CPKCVDREKAIEEWKEYVSEHKELKEHFSELFEEKKVESEQENPLKQKEFERTKNHPA
                     NPHNFVPGQRRSGHQKKIG"
     gene            1508..4633
                     /gene="ileS"
                     /locus_tag="PLW08_06965"
     CDS             1508..4633
                     /gene="ileS"
                     /locus_tag="PLW08_06965"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPL52377.1"
                     /translation="MYKTIDLKESPRHLENRIREYWNKHDMVQKSIDIREGMPRFIFY
                     EGPPTANGKPGIHHIMARTLKDVICRYKTMNGFQVKRKAGWDTHGLPVEIEVEKMLGL
                     KDKRGIEEYGIEKFCQKCRESVFSYEKLWREMTELMGYWIDLDHPYVTLHNSYIESVW
                     WILHNFFSRNLIYKAYKIVPYCPSCGTPLSSHEVAQGYKDIEDPSIYVKFKATDEENT
                     YYLAWTTTPWTLISNVALAVHPEETYLKVEYQGQNLILAKSRLQVLDEETKIISEMQG
                     RELEGRCYQPLFNFVSVDKPAWYICLGDYVTMSEGTGIVHTAPAFGQDDYSLALKYDL
                     PFVQPVDSEGKFTEAVTPWAHQFVKDADKDIIRHLKERGNLLKREQIKHSYPFCWRCK
                     SPLIYYARESWYIKTTAYKEQLIENNRKIIWYPPFVGEKRFGDWLENNVDWALSRDRF
                     WGTPLNIWICEKCGKLRSLGSIEELRKEGKMTSGKDVPEDLDLHRPYIDEVVLTCSCG
                     NKMYRTPEVIDCWFDSGSMPFAQWHYPFENYENFEPELFPADIISEGIDQTRGWFYSM
                     LAISTLLKGVSSYKSCLVNELILDKNGRKMSKSHGNAVDPIELMQNYGADAIRWYLLE
                     VSPPWVPTRFDVDGVKEILGKFIGTLKNVYSFFATYANIDGFVAINYPYNWEREVEID
                     RWIVSRLHSLIITVRHYFESYDFTKAVRAIQSFVIDELSNWYVRRSRRRFWASAFDQD
                     KIDAYRTLYQVLVETAKLIAPFAPYLAEELFLNLEAGESVHLELYPEANKTYIDTKLE
                     KKMQTVIDIVSLGRAARNECQIKVRQPLNEMFVPTKVKDDLADMIDLVKEEVNIRNIT
                     FVSEQDGFVHYELKPDFKLLGPKYGKKVQIINALLLQTPAENVLQAFNTTGSYTLEIE
                     GETIHLLPEEISVTIIPREGYVFANAKDLFVALDTTLTEELLNEGYARELINKIQFTR
                     KEMNLDIMDNIIVKLVVPEAIAKAIQVHKNYILTETLGKELVFLEKPMANMQCLDING
                     NKIHLGIEKAV"
     gene            4869..6656
                     /locus_tag="PLW08_06970"
     CDS             4869..6656
                     /locus_tag="PLW08_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPL52378.1"
                     /translation="MLNKDAEGIKYIVEKEGREFLLKIFYKTSLSNIENIFSLQQRLQ
                     ELNKLENKQIPKVTEIDPHYDPPYMVVEFIHGISLANLKTHNPKKLTEDFIRLVAIQI
                     VNTAISLHQHQLTLSQLALTNIMINDNDEVIILSSAITWEERDEREEMFSIALVLAQA
                     LSQHNFYKTLYSKERLTTQKFEYIYGVSVPLNKILAECLHRNILQRYRSLDDLLNALI
                     NLPPVSECEIWTEPEKAIPDNIEDRSKKDIPKTRIELPFWILIALIIVTVVMLLTTNI
                     FSVLFGGKGEKLTYSSWLMPNSESDTVNSSVPIAELPREKPTQTTYGELKTGTPTPRE
                     DFRKQPSVPKITTVIPPPTAPKPRPPQNMVFIEASTLGFGRLRENPNPNVSLSSFYIN
                     KFEVTQEEWNIYMKPANCSTFGENLPVDNISWFDIAIYCNGRSEAEGLTPAYKIRGVG
                     ASRVVTCDFKANGYRLPTEAEWEMAAKAGKLYNYSGSDDPDEVSWNRDNSGGKIHTPG
                     TKKPNDFGIYDMTGNVAEWVWDWYDVNYIRSLPTFINPTGPETGTLKVIRGGSVMNGE
                     GRNLNIMWREKGDPNRGYQYVGFRLVRSN"
BASE COUNT         2193 a         1254 c         1316 g         1931 t
ORIGIN      
        1 gagatatgtt gtttttattt actcttttca tcgggttaaa acccgtcgtt aatagctatc
       61 gtttttaagg aactgatact tgaaatcttg taaatccctt ttatcctgtt catcccctac
      121 ctattattct caaagctcta agctctctgc cccctgctaa aatcatccta ttcatcccat
      181 acttattatt ctcaaagctc taagctctct gccctctgcc aaaatcatcc tattcatccc
      241 atacctattc tcatttttaa tctgtgttat ctgcgaaatc tgcgtgctat tatcgttaat
      301 tttcttgaca gcataagtca ttcatcagac aaggaaaagt tatgaaaata gctcggcatt
      361 ttttctgtga actgaaacgc aaccgtgaac ctgcaccaca attgtgtgcg gtttgccctc
      421 gtattctcaa atgcaagagt ttttatgcct ggcacaaatt acatcaccaa gaatatctaa
      481 atttcgtaat tgaaatcact aaaaaattcc ctgataaata tacaatggag gttcacttta
      541 tgcctgaaaa acaaaccttc gtccaaattg tggatatggc taccggaaaa atagaaaagg
      601 tagtcagttt gacggaaatt gaggcattat ccgcagagga taaacttgct ttatcccgca
      661 ataagaactt atttattgta acgcaccgtt tggaaccaat tgtaaaaatt gaacttaaga
      721 aaacggtgat tagtgctccc attcaattta tggaaagcgc tcctaaagag gaagagccaa
      781 ttgaggaggt tacgccttta ccgataaacc cggaaaagcc aaaaccaaaa cgcacaccca
      841 agaactgaat atttctgagc tctcattttt atttgacaga ggattatctg tttaatcata
      901 gtgagatgct gaattctgtt acagaagata caaagaatgg actacttatg tgtctttttg
      961 ttttctgtgc ttgaagttga aatttgtcac cctatattta tttagaagct cctgtaaaca
     1021 gaagaggcga aaatttcctt ttacagaatt ctgcactaaa aaaaaatggc tcaaaatgag
     1081 ggaaattgtg gagagagata tataaacctc tctgtatagt aagcaaagga ggaaatatgt
     1141 ttatccttga cgaaatacat ttggatgtgg caaaagccat agccgaaaaa aatcgtaaga
     1201 aaaaaagttg tgatcactgt tacgacagag gttggatcgg tgttagtgaa caaaatttgc
     1261 ttgttctctg tccaaaatgt gttgatagag aaaaggcaat tgaggaatgg aaagaatatg
     1321 tttctgaaca taaggaattg aaggagcact tcagtgagct ctttgaagaa aaaaaagtgg
     1381 aaagcgaaca ggaaaatcct cttaaacaaa aggaatttga gcggactaaa aaccatcctg
     1441 ccaatccgca taattttgtt cccggtcaac gcagaagtgg tcatcaaaag aaaattggat
     1501 aaggtgtatg tataaaacaa tagacctgaa agaaagtccc aggcatctgg aaaaccgcat
     1561 tcgcgaatat tggaataaac acgatatggt gcaaaaaagt attgatattc gtgaaggtat
     1621 gccacgcttc attttttatg aagggccccc tacagcaaat ggcaaaccgg gtattcatca
     1681 tataatggca aggaccttga aagatgttat ctgccgttat aaaacaatga acggctttca
     1741 ggttaaacgc aaagccggtt gggatactca tggtttgcct gtggaaattg aagtggaaaa
     1801 aatgctcggt ctgaaagata agcgcggtat agaggaatat ggaattgaaa aattctgtca
     1861 aaaatgccgg gaatccgttt tcagctatga aaagctttgg cgtgaaatga cagaactgat
     1921 gggctattgg atcgatctgg atcaccctta tgtaactttg cataatagtt atatagaatc
     1981 cgtttggtgg attttgcata attttttttc ccgaaacctc atttataaag catataaaat
     2041 tgtcccttat tgccccagct gtggaacccc tctttcttca catgaagtag ctcagggtta
     2101 taaagatata gaagacccct ccatttatgt taagtttaaa gcaacggatg aagaaaacac
     2161 ctactacctg gcttggacaa ctactccctg gactctaatt tccaatgttg ccttagctgt
     2221 ccatcctgaa gaaacctacc ttaaagttga atatcagggt caaaatctca ttttagctaa
     2281 atctcgctta caagtgctgg atgaagaaac aaagattatt tccgaaatgc aaggcaggga
     2341 actggaaggt cgttgctatc agcccctttt taattttgta agtgttgata aacccgcttg
     2401 gtatatttgt ttgggtgact atgtaacaat gagtgaagga accggaattg tgcatacagc
     2461 tcctgcgttt ggtcaggatg attattcttt agcattaaaa tacgatttgc cttttgttca
     2521 accggtagat agcgaaggta aattcactga ggcagtaact ccttgggcac atcagtttgt
     2581 taaagatgcc gataaagata ttattcgtca cttgaaagaa agaggcaact tattaaaaag
     2641 agaacagata aaacatagct accccttttg ctggagatgt aaaagtcccc ttatttatta
     2701 tgcgcgtgag agttggtata ttaaaaccac ggcttataaa gaacagctaa tagaaaataa
     2761 ccgcaaaatt atctggtatc caccttttgt aggagaaaaa cgctttggtg actggctgga
     2821 aaacaatgtg gattgggctt tatcccgtga ccgtttttgg ggcacacccc tcaatatctg
     2881 gatttgtgaa aaatgtggaa aactgcgttc tcttggttct attgaagaat tgcgcaaaga
     2941 aggcaaaatg acctccggaa aagatgttcc ggaagacctt gatttacacc gtccctatat
     3001 tgatgaagtt gttcttacct gttcctgcgg aaataaaatg tatcgcacgc cggaagttat
     3061 tgattgctgg tttgatagcg gttctatgcc ttttgctcaa tggcattatc cttttgaaaa
     3121 ttatgagaat tttgaaccgg aacttttccc cgctgacatc atttcggaag gaatagacca
     3181 aactcgcggt tggttttatt caatgcttgc catttctact ttgctgaaag gtgtttccag
     3241 ttataaaagt tgtttggtaa atgaactgat tctggataaa aatggcagaa aaatgagtaa
     3301 atcacacgga aatgcagttg accctattga actgatgcaa aattatggag cggatgcaat
     3361 tcgctggtat cttttagagg tctccccccc ttgggttccg acgcgttttg atgtagatgg
     3421 cgttaaagaa attttaggca agttcattgg tactttaaaa aatgtttatt ctttctttgc
     3481 cacttatgcc aatattgatg gttttgtggc aattaactat ccttataatt gggagcggga
     3541 agttgaaata gaccgttgga ttgtttcccg tttgcattca cttattatta ctgttcgcca
     3601 ctattttgaa agttatgatt ttaccaaagc ggttagagct attcaaagtt ttgttattga
     3661 cgaactttcc aactggtatg tgcgaagaag tagacgccgt ttttgggctt cggcatttga
     3721 ccaggacaaa atagatgcct atagaaccct ctatcaagtt cttgtagaaa ccgctaaact
     3781 tattgctcct tttgctccct atctggcaga agagctcttc ttaaatctgg aagcaggcga
     3841 aagtgtccat ctggaattgt atcctgaagc aaataagacc tatatagata ccaaactgga
     3901 aaagaaaatg cagaccgtta tagatattgt ttctttaggc agagctgcca gaaatgaatg
     3961 ccagataaaa gtgcgtcaac ccttaaacga aatgtttgtt cccaccaaag tgaaagatga
     4021 tttggcagat atgatagacc tcgtaaaaga agaagttaat attcgtaata tcacttttgt
     4081 aagcgaacag gatggctttg tgcactacga attgaagccc gatttcaaac tgctgggtcc
     4141 taaatacggt aaaaaagtac aaattattaa cgctcttttg cttcaaaccc cggcagaaaa
     4201 tgtcttgcag gcatttaata cgaccggttc ttatacctta gaaattgaag gtgaaactat
     4261 ccatctctta ccggaagaaa ttagtgtaac cattattccc cgtgaaggtt atgtatttgc
     4321 taatgcgaaa gacctctttg tagctttaga tactacctta acggaagaac tcttaaacga
     4381 gggctatgcg cgtgaactga tcaataaaat ccaatttacc cgcaaagaaa tgaatttgga
     4441 cattatggat aatattattg taaagctggt agtcccggaa gcaatcgcca aagcaattca
     4501 ggtgcacaag aattatatct taacagaaac tcttggaaaa gaactggttt ttctggaaaa
     4561 accaatggca aatatgcaat gtctggatat caatggcaac aaaattcact tgggtattga
     4621 aaaagctgtc taataagggt taggaggcaa tcattaataa atattgtatc aattgtggaa
     4681 agcttatacc cgaaaaagca aaattctgtc ctgtctgcgg agaaaatcaa atccaggaac
     4741 cggaaaagcc aaagcaaaca gaaaccaaaa aagaaaaagc acaaaaatct gcagaagcaa
     4801 tttcccacag aaattctcat ttacttaaga atcctggtag caccttcagg gattatacca
     4861 taattagaat gctgaataaa gatgctgaag gaataaaata cattgtggaa aaggaaggta
     4921 gggaatttct gctgaaaata ttctataaaa caagcttaag caacatagaa aatatttttt
     4981 ctctccagca acgcctccaa gaactaaata aactggaaaa taaacaaatt cctaaagtta
     5041 ctgaaattga tccgcattat gatcctcctt atatggtagt agaatttatt catggtattt
     5101 ctcttgcaaa ccttaaaacc cataatccga aaaaattgac tgaggacttt atccgcttag
     5161 tagcgatcca aattgtaaat acagcgatta gtttacatca acatcaactt acgctcagtc
     5221 aactggcttt aaccaatata atgataaatg ataacgatga ggtgataatt ctttcttccg
     5281 ctatcacctg ggaggaaaga gatgaacgtg aagaaatgtt ctctattgct cttgttttag
     5341 ctcaagcatt atcccaacac aatttctata aaacattata cagcaaggaa cgtttaacta
     5401 ctcaaaaatt tgaatatatc tacggagtaa gcgtccccct caacaaaatc cttgctgaat
     5461 gtttgcatcg caatatttta cagcgttatc gcagtttgga tgatttactg aatgccttaa
     5521 tcaacctgcc tcccgtttct gaatgcgaaa tttggactga accggaaaaa gcaataccag
     5581 acaatataga agaccgtagc aaaaaagata ttcccaaaac cagaattgaa ctcccctttt
     5641 ggatactgat tgcactcatc atcgtaactg ttgtaatgtt gcttaccacc aatattttca
     5701 gtgttctctt cggtggcaaa ggagaaaaat taacttattc ctcatggctt atgcctaata
     5761 gcgaatcaga tactgtaaat agttctgttc ctatagctga attgccccgc gaaaaaccaa
     5821 cccaaacaac ttatggtgaa ttgaaaaccg gcactccaac ccccagagag gactttcgta
     5881 agcagccctc tgttcccaaa ataactactg ttattccacc acctacagct cccaaacctc
     5941 gtcctcctca aaatatggtt tttattgaag caagtacttt aggttttgga cgcttaagag
     6001 aaaatcccaa tcccaatgtc tccttatctt ccttttatat caataaattt gaagttacac
     6061 aagaagaatg gaatatttat atgaaaccgg caaactgttc caccttcgga gaaaacttac
     6121 ctgtagataa catcagctgg tttgatattg ccatttattg caatggcaga agtgaagcgg
     6181 agggtttgac cccagcatat aaaattagag gagtgggtgc ttcgcgagtt gttacctgcg
     6241 attttaaagc taatggctac cgtctgccta ctgaagctga atgggaaatg gcagctaaag
     6301 caggtaaact atataattat agcggatcgg atgaccccga tgaggtgtct tggaacagag
     6361 ataacagcgg cggtaaaatt catacaccgg gaaccaaaaa acccaacgat ttcggcattt
     6421 acgatatgac aggaaatgtt gcagaatggg tttgggattg gtacgatgta aattatatcc
     6481 gttccttgcc tacttttatc aatcctacag gtcccgaaac tggaacccta aaagttatcc
     6541 ggggtggaag tgtaatgaat ggagaaggta gaaatctaaa cattatgtgg agagaaaaag
     6601 gagacccgaa ccgggggtat caatatgtgg gctttcgctt agtgcgcagc aactaatatt
     6661 tcttatacaa aaaaagaaaa gaagagaaaa agcc
//
LOCUS       DAOOZV010000078         6618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_10283_length_6618_cov_59.712479, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000078 DAOOZV010000000
VERSION     DAOOZV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10283_length_6618_cov_59.712479"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..226)
                     /locus_tag="PLW08_06975"
     CDS             complement(53..226)
                     /locus_tag="PLW08_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPL52379.1"
                     /translation="MPDRTLICQDCSREFVFTEGEQEFYKEKGLQNEPKRCPECRKAK
                     KAQFNRNRRRQSN"
     gene            complement(369..1643)
                     /locus_tag="PLW08_06980"
     CDS             complement(369..1643)
                     /locus_tag="PLW08_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPL52380.1"
                     /translation="MNSEKIVQYIQKHCIADDWTLKITKDDSHETRFAQNVITQHIAG
                     AMKEISLSVSFGSKSGSCTVNQDDEESLAYLVKTAEEIAKLAPEDPEFVPSVGKMDIP
                     EIANCDPQTKALEPKQLVDIVQTSINKAKTFGATVSGLTEKHIETNALFTKNGFAGEY
                     EKSDFGHSMTLKKEEVETKVAYEAKNFADFNLQILLEKLLNQASSLAVKRTFEPQKIA
                     VLLRPLALQELFWFMGWMMDRRYADEGITPFTNQIGNPFFGEKFSWFSTCKQPTLLAP
                     PFAHDGIIAEEIPWVEKGILKNLATSRYWAKKIGSKPCDIYNMFIPGEGYSEEEMLQM
                     VPRGLIVNSFWYIRTVDTKAGEFTGTTRDGVWYFEDGKIQYAVNNLRFNEIPHNATGR
                     IIATGISELANPISLLPPMLIDNFNFVDKTSF"
     gene            complement(1640..3082)
                     /locus_tag="PLW08_06985"
     CDS             complement(1640..3082)
                     /locus_tag="PLW08_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPL52381.1"
                     /translation="MKYLDLAMNAAEKFGAEYADIRIQKTTDQVIYLQNLSLKHTSNN
                     VLQGYGIRVFKDGAWGFAHSNVFNEEAVLATVKRAVETATLSAEVNKTQKLRLAPERS
                     YIATYRTPVKIDPFTVPVSEKIDLMMEVARTMLAYEGIMKTVFLMILHKDEKLFASTL
                     GTRLDLTTQFINPMITAVAVTESDSQSRTFDEGGRAVGWEWILELDLREKAKQIAEEA
                     IIKVKADTLGAEERRSLILDPNHLGLTMHESVGHATELDRVLGWEADYAGVSFATPEK
                     LNNYHYGSELINFVGDNTLEEGLATLGFDDDGVPGQKWYIIKDGILKEYGSTRDTALE
                     IGLNASRGCNRATYYFNQPINRIPNLYLLPGTKPLTPGELIADTEDGVYIQGRGSYSI
                     DHHRINFQFGGDFFWEIKNGKLIRPLKKVIYKSYNPEFWNSCDAICDERFWRPFGVVN
                     CGKGQPPQTGRMTHGASTARFRNIRVGGSQ"
     gene            complement(3098..3388)
                     /locus_tag="PLW08_06990"
     CDS             complement(3098..3388)
                     /locus_tag="PLW08_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52382.1"
                     /translation="MMQFYESPPYSMTNKIEETPLAPAMKISDWIIVLILTCIPVLNL
                     IMLIIWSLDKRGNPNRRSFAQAVLIFVAVMLVLSSFYFGQMTGWLFQIMALM"
     gene            complement(3656..4189)
                     /locus_tag="PLW08_06995"
     CDS             complement(3656..4189)
                     /locus_tag="PLW08_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52383.1"
                     /translation="MKKFIVLPVLLLLLLSSCEEITKVTVRDDNQDQVAHLGPDPDLL
                     GNTELFFIPGTIQGSAIWVINGPAANIGVDIRDKDSSSFIYFADSYIGSGSNTAQTGT
                     QIPWNKWMKVRVVVYKSGLSGIIVNIIRALGLDFFDSLEDYMIEQIFENEVYLSKDGT
                     RLTTPVKSYYKLQELNL"
     gene            4513..>6618
                     /locus_tag="PLW08_07000"
     CDS             4513..>6618
                     /locus_tag="PLW08_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPL52384.1"
                     /translation="MKQAVIILLLLITCLSPLWGMRKALVIGNSNYKEQVLRNSINDA
                     SDVYNALKGLGFSASLFTDLNREGFFRAVGEFVSTLTPSDEVVFFYSGHAAQIEGKNY
                     LIPVNEYIDSATRCAYLSYDTSMLLDELSRAAISIVILDACRDNPFAFSRSIRSGLAP
                     MTVSAGSQCVIFATEQGRTASDGEGRNSPFTESLLANITTPGIKITDLIQRVSNEVAI
                     KTQERQIPYSTGTLRQDFYFVKAEPVTPISPVIPPSIPQEPKPVVSPSYGSIKVKTSF
                     GGDLYIDGEFSQSLFAGDQSIIKDIAVGVYELKLVSPKETIIRLANVNKNQTTMIDFD
                     APDSYREEPKKIPEQQIIIPEQQIPHSEKQSIELNSLTPLSPITKKVSVGTGALTIES
                     PLAGDLYLGGDFIGKLAAKTSKKFSNVQAGSYTLEVMHQNSFCRKEIRIYNKDNLKLN
                     LSQTEFSKYPNGFQYVSGGTFQMGTQFLNRSEDERPRHEVQILPFLMKKTEVEQKLWT
                     DVMGYNPSEIKGDSYPVTNVSFYEAVQFCNALSTLNGLTPCYKIDKSKTDPTNLCNLD
                     TLRYIISCNWNASGYRLPTEAEWEYVSRCGDNYNKLQFSGSNNANDVAWNSKNSENKI
                     HPVATKNPNQWGFYDLSGNVFEWCWDYYDIYKKDKQVHPKGAEKGVYRIARGGSFNSD
                     IDACSSTARGGFAPNFKGNN"
BASE COUNT         1982 a         1349 c         1191 g         2096 t
ORIGIN      
        1 aaacaaaaac gccactacat tttagcagtg gcgttaacag attagaaact atctaattag
       61 attgtctgcg tctgttacga ttaaactgtg ccttttttgc cttacgacat tcggggcagc
      121 gtttgggctc gttctgaagg cctttttcct tatagaattc ctgttcacct tcagtgaaaa
      181 cgaattctct ggaacagtcc tggcaaatga gtgttctgtc tggcatttgg tatctccttt
      241 tattaacggg atcgaacatt ctggtccaaa ggcactcccg taatagaagg aattggctta
      301 gggcgaatgt tgaatcgcat ttgtagatac ctgtttttaa ttttcacatt tctgtcaatt
      361 tattttttct aaaaagaggt cttgtctaca aaattgaagt tatcaataag cattggaggc
      421 aacaaagaaa taggatttgc tagctcactg atacctgtag caataattct tccggttgca
      481 ttatgaggaa tttcattaaa gcgcaaatta tttacagcat attgaatttt tccatcttca
      541 aaataccata caccatcgcg agtggttcct gtaaattcac ctgcttttgt atccacagtg
      601 cgaatatacc aaaagctgtt tacaataagc ccgcggggaa ccatttgcag catttcttct
      661 tcactatatc cttcaccagg aatgaacata ttatagatat cgcaaggttt actgccgatt
      721 ttttttgccc aataacggct ggtagcaagg tttttcaaaa tgcccttttc cacccaggga
      781 atttcttcgg caattatgcc atcgtgagcg aaaggtggtg ctaaaagagt tggctgctta
      841 caagtagaaa accagctgaa tttctctccg aagaaggggt ttccaatttg gtttgtaaaa
      901 ggagtgatac cttcatccgc atatctgcgg tccatcatcc aacccataaa ccaaaataat
      961 tcctgaagag caagaggtct taaaaggaca gctatttttt ggggttcaaa tgtccgtttt
     1021 acagcaagag atgatgcctg attgagcagt ttctccaaaa ggatttgtag gttaaagtcc
     1081 gcaaaatttt tcgcttcgta ggcaaccttt gtttctactt cttctttttt cagtgtcata
     1141 gagtggccaa aatcactttt ttcatattca ccagcaaaac catttttagt aaatagagca
     1201 ttggtttcaa tatgcttttc ggttaaaccg gaaactgttg ctccaaatgt ctttgccttg
     1261 ttaatgctgg tttgaacaat atctactaat tgtttaggtt ccagagcttt agtttgagga
     1321 tcacaatttg caatctccgg aatgtccatt ttccctacgg aaggaacaaa ttcagggtct
     1381 tcgggagcta atttggcaat ttcttcagct gttttaacaa ggtatgccaa actttcttca
     1441 tcatcctgat ttacagtgca gcttcccgat ttactaccga aagaaacgct taaagaaatt
     1501 tccttcattg ccccggcaat atgttgagtg ataacatttt gagcaaagcg tgtttcgtgg
     1561 ctgtcatctt tagtaatttt tagtgtccag tcatctgcta tgcagtgttt ctggatgtat
     1621 tgcacaattt tttcgctatt cattgtgaac ctcctacccg gatattacga aatcttgccg
     1681 ttgaggcacc atgagtcatt cttcctgttt gtggcggctg acctttgcca caatttacaa
     1741 ccccgaaggg acgccaaaaa cgctcatcac aaatagcatc acaactattc cagaattccg
     1801 gattgtaaga tttgtaaata acttttttca agggacgaat cagtttgccg ttttttattt
     1861 cccagaagaa atcgcctcca aactggaaat ttatgcgatg atgatcaata gaataactgc
     1921 ctctgccttg aatataaact ccgtcttcag tatcggcaat aagttcaccc ggagttaagg
     1981 gttttgttcc tggcagtaaa tataaattgg gaatgcggtt aatcggctga ttaaaataat
     2041 atgttgcacg attacatccg cgggaagcat tcaaaccaat ttccaaagca gtatcacgtg
     2101 tgctgccata ttcttttaat ataccgtctt taataatata ccatttttga ccaggaaccc
     2161 catcatcatc aaaaccgaga gtggcaagac cttcttccaa agtgttatct cccacaaagt
     2221 taatcagttc actaccgtaa tggtagttgt tcaatttttc cggtgtggca aaagaaacac
     2281 ctgcataatc ggcttcccaa cctaaaactc tatccagttc ggtagcatga ccaacgcttt
     2341 catgcattgt taagcccaga tgattaggat caagaattaa agacctgcgt tcttcagcac
     2401 caagggtatc cgctttcact ttgataattg cttcttcggc aatttgttta gctttttccc
     2461 ttaaatccag ttccaaaatc cattcccaac cgacagctct tccaccttca tcaaaagttc
     2521 ggctttggct gtcactttcc gtaaccgcta cagccgtaat cataggattg ataaactgcg
     2581 tagtaaggtc taaacgcgtt cccaaagtgc tggcaaagag tttttcatcc ttatgcagaa
     2641 tcattaaaaa cacagttttc ataattcctt cataagcaag cattgtgcgg gcaacttcca
     2701 tcatcaggtc tatcttttca gatacgggaa cagtgaaagg atcaatttta acgggagttc
     2761 tgtaagtagc tatgtaactg cgttccgggg ctaaacgcaa tttctgtgtc ttattcactt
     2821 ccgcagaaag ggttgctgtt tcaactgccc tttttacagt agctaaaaca gcttcttcat
     2881 taaaaacatt gctatgcgca aaaccccatg caccatcttt gaaaacccga atgccataac
     2941 cctgcagaac attattactg gtatgtttca gggaaaggtt ttgcagataa attacctgat
     3001 cagttgtttt ttgaatgcgg atatcggcat attcagcacc gaatttttcc gccgcattca
     3061 ttgccaaatc taaatatttc atactttctc cttatttcta cattaaagcc attatttgaa
     3121 aaagccaacc agtcatctgc ccgaaataaa agctggaaag gactaacata acagccacaa
     3181 aaattaaaac tgcctgggca aaactacggc gattgggatt gcctctttta tccaaagacc
     3241 atattatcaa cataataagg tttaataccg gaatgcaggt caggatcaaa acgattatcc
     3301 aatcacttat cttcatagca ggagctaagg gtgtctcctc tatcttatta gtcattgaat
     3361 aaggagggga ttcataaaat tgcatcattc atctcctatt gtttacttaa aattttttcc
     3421 tttattccct aactacaaaa tccgtcaagt tttatcatct gataccattt ctttactgat
     3481 aagccatttg tcattcctgc acaggcagga atccgcataa aacaaaaaac atttatatat
     3541 gctcaatagg atagttatct tacctgtaag caaggagaat tttctccagt tccttttttg
     3601 ccaacttata taaaggcaat aagatatacc tctgtattac ttcaatatcc tgtgtttata
     3661 ggtttaattc ctgaagcttg taataactct ttacaggggt tgtcagccgg gttccgtctt
     3721 tgcttaaata gacctcattt tcaaatatct gctcaatcat atagtcctct aaggaatcaa
     3781 aaaagtccaa acccaaagcc cgaataatat tcactattat gcctgaaagt cctgatttat
     3841 aaaccactac acgaactttc atccatttat tccagggtat ctgagttcct gtttgagcag
     3901 tgttagaacc tgaacctata tagctatcgg caaaatatat aaaagacgaa ctatctttat
     3961 cccgaatgtc aactcctata tttgctgcag gaccatttat tacccaaata gctgaaccct
     4021 gaatagtgcc aggaataaaa aacaattccg tattaccaag caaatcggga tcgggaccta
     4081 aatgcgctac ctgatcctga ttatcatccc gaacagttac tttagttatt tcctcacaag
     4141 aggataacaa aagcaacaat aagaccggta gaacaataaa ctttttcata aaaactcctt
     4201 aactaccaat catcaaaata attaatcaat ttacttctta aggggaactc taaaaattgt
     4261 caatgtttat ttttcctatc tctgatgcgt gttttccctt tttattcttc attatcctgt
     4321 agacctcttg acttttctat tgacagtttt tcctcaaaca attaataagt aaaaatagaa
     4381 tataagagct attggacatt gtgggcgaaa atgatctgca gttaatataa ggtctatttg
     4441 ctttctttcc aatatcggtt ataatattta aagaggtctt atagcaaaac cactttatct
     4501 aaggaggtgg ttgtgaaaca ggcagttatt attctacttc tgcttattac ttgtttaagc
     4561 cctttatggg gtatgcgcaa agcattagta attggaaatt ccaattacaa ggagcaggtt
     4621 ttgcgcaatt ctataaatga tgcttcagat gtgtataatg ctcttaaagg gttagggttt
     4681 agcgcttctt tatttactga cctcaatcgg gaaggttttt ttagggcagt tggtgaattt
     4741 gtttctacac ttactccttc cgatgaagtg gtcttttttt atagtggcca cgctgcccaa
     4801 attgaaggta agaattatct tattccggtg aatgaatata ttgattcagc aacgcgttgt
     4861 gcttatcttt cttatgatac ttcaatgctt ctggatgaat tgagcagagc tgctataagc
     4921 attgtaattt tagatgcctg ccgagataat ccttttgctt tttcgcgttc cattagaagc
     4981 ggtttagctc caatgacagt ttcagccggc tctcaatgtg taatttttgc tacggaacaa
     5041 ggaagaactg cctctgatgg agaaggtaga aatagtcctt tcacggaatc cttgcttgcc
     5101 aatattacca ctccggggat taaaattact gatcttattc aaagggtcag taacgaagta
     5161 gcgattaaaa cccaagaacg gcaaattccc tatagcacag gaactttaag gcaggatttc
     5221 tattttgtta aggcagaacc tgttacaccc atttctcctg ttattcctcc ttctattcct
     5281 caagagccca aacctgttgt ttctccaagt tatggcagca taaaagttaa aacctctttt
     5341 ggcggggact tgtatataga tggtgaattc agccaaagtt tgtttgcagg agaccagtcc
     5401 attatcaaag atatagcggt tggagtttat gaactgaaat tggttagtcc gaaggaaaca
     5461 attatccgtt tagctaatgt taataaaaat cagaccacaa tgattgattt tgatgctccc
     5521 gattcttata gagaagaacc taaaaagata ccggagcaac aaataattat tcccgaacag
     5581 caaattccgc attccgaaaa acaatctata gagctcaatt ccttaactcc actatcacct
     5641 ataaccaaaa aagtgtcagt gggcacaggt gctttaacca tagaaagtcc tcttgctgga
     5701 gacctttatt tgggaggtga ttttattggc aaactggcag caaaaacctc taaaaagttc
     5761 tctaatgtcc aagctggttc ttacactttg gaagtaatgc accaaaattc tttctgtcgt
     5821 aaggaaatta gaatttataa caaggataac ctaaagttaa atctttccca aactgagttt
     5881 tccaagtatc ctaatggttt ccaatatgtt tccggcggaa cttttcagat gggaactcaa
     5941 tttttgaatc gcagtgaaga tgaacgtcct cgtcatgaag tgcaaatact tcctttcctt
     6001 atgaaaaaaa cggaagtgga acaaaaatta tggactgatg taatgggcta taacccttct
     6061 gaaattaaag gcgatagtta tcctgttacc aatgtttcct tttatgaagc agtgcaattt
     6121 tgcaatgccc taagtacttt aaacgggtta actccttgtt ataaaataga taaatccaaa
     6181 actgatccta ccaatctctg caatttagat actttgcgtt atatcatttc ctgcaactgg
     6241 aatgcctcag gttaccgttt acctactgaa gcagaatggg aatatgtatc tcgttgcgga
     6301 gataactata acaagcttca attttccgga agcaacaatg ccaacgatgt cgcctggaat
     6361 agtaaaaaca gcgaaaacaa aatacatccg gtggccacta aaaatccaaa tcaatggggc
     6421 ttttatgacc tcagtggtaa tgtatttgaa tggtgttggg attattatga catctacaaa
     6481 aaagataaac aggttcatcc caaaggagcg gaaaaaggag tttatcgcat tgccagaggc
     6541 ggaagtttta atagtgatat agatgcctgt tcttctactg cccgaggtgg ttttgctccc
     6601 aattttaaag gaaacaat
//
LOCUS       DAOOZV010000079         6613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_10295_length_6613_cov_59.067551, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000079 DAOOZV010000000
VERSION     DAOOZV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10295_length_6613_cov_59.067551"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLW08_07005"
     CDS             <1..684
                     /locus_tag="PLW08_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52385.1"
                     /translation="QPEDMDYPIACQVTPVDQYGLAGTPVLSSPTIPIEDLPAPQNLT
                     GTLTPPNTVTLHWEKPIHYQGKVFSGYKIYRDEAVLYNLMNPDSLSYVDLNVPDGTHT
                     YWVCSIFTYPLVFSSPSNAVTISINVPNSDEIAPALKSVSVYPNPFKTTTNFQISGKA
                     NRPVTIEIYNLKGQLIKRLATKTDQNGLAQISWIALDENAKPVRNGIYFYRTICDSSS
                     ETGKLILLK"
     gene            complement(1092..1265)
                     /locus_tag="PLW08_07010"
     CDS             complement(1092..1265)
                     /locus_tag="PLW08_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52386.1"
                     /translation="MDGLKEIQGVMAAAFSGMKLQDKSYAPVIGLIWQITLWTLFNYL
                     LPGHLFNDSLTIL"
     gene            complement(1366..4098)
                     /locus_tag="PLW08_07015"
     CDS             complement(1366..4098)
                     /locus_tag="PLW08_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPL52387.1"
                     /translation="MKKLIIWLIILLSCQFALALNDNIYFINARQYYELGNYASAKQN
                     LDMIQAPDNEDPEYALLCGKVHLALGDYKQAHIWLSEYKKKSLGADPLVQEDLLQMLY
                     EVALYQEQTSVSVSLGKLKGSINTSDSEYAPVFTPDGKYMYFSSLRRSNFGKENIFLS
                     QQQNNVFSEAIEVDELCTDYNESFGSLSMDGNTAYLFGYYSKDTTNGDIYQTTLDSKG
                     RWTKPLMIKEVSSKYYDLQPFIWRDKVMFLTSNRDGDHKNYDIYVSENINGIWTTPVS
                     VGDIVNTPYDEQSPFLSPDGKYLYFASFGHNSYGGNDIFVSTRIGNSWTEWSKPVNMG
                     PIINSVKDDRYFVIAPDGRTAYLCSNRAGGMGQEDIYTIDLGLLDKMKEQIILLTGVK
                     PTEEQPVIPQVFKELKISGVVVDDKNQPVKTDIIWVYDLGDKTFMRIIGSDELGTFSF
                     TLPGDTKDIAFEINTPGYKKTTGKIEIPPDKNEIFVNITLPVETGIGEGGNLAINGKV
                     LDEENNPVPCSIRWSYVFEGELTEVIVESNNEGVFKLYTPIMEKLKYRIEEPGYAVRE
                     EIITIPKNVNSYDTIIRLVRLGKEVTLSGKVFDDNDNPLVAMVTWIYESENEIIEYRV
                     VTDSNGDYSISLPRMPKMQYRVSKPNYLQVSGEINIPEDQQNITKNFRLSKLEKEAVF
                     ELENVEFEFNKAVLTPRSLEILKPVLATMKENETLEIELSGHTDNIGSKQYNQKLSEA
                     RAKAVADYLIKNGIDPKRIKTVGYGFDKPIASNDTPEGRQRNRRTEMKILGIEYMEDT
                     YEDIEKEFSQAEKKARVVKTISKDSMYAVTQTGIPASLENQFKTMIHQTLADKKQFNI
                     KVDLFLDNGKIQSANVRDLLGNLEEQTTEKIADMMLGWKVQSQGRSIYSFTVEK"
     gene            complement(4203..5159)
                     /locus_tag="PLW08_07020"
     CDS             complement(4203..5159)
                     /locus_tag="PLW08_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52388.1"
                     /translation="MKQIKLVLIALILMISLSSVFAEDNSAAVYQLLGIDARSNGMGG
                     TGTAFLDNVSSAYLNPAVLADVKTIEFTSGTRQNMAWDRNHNAVALGFVLPIGYVAAS
                     WQNATVSDIPGYDVNGNPTGDFSNSDNTLALSYAAKISKLNLGITPKFYLSNQDNESD
                     TGYGVDLGFLYHINRYFNVGMVVRDLIANYPGEEEDIPIEFIPGVAAYPIPGLIIAAD
                     LSGRDNFEESKLRLGVEYWLGVKDDAEIGSSLSGIRIRESATWSDILSKVQAGIRAGV
                     NDGAFTAGAGLRFKMLEMNYAFQMAHDEYLNDTHSVSLLLRF"
     gene            complement(5216..>6613)
                     /locus_tag="PLW08_07025"
     CDS             complement(5216..>6613)
                     /locus_tag="PLW08_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPL52389.1"
                     /translation="IPVDQTGIRLEVTATNILDGTSTSNQVYGIDNSAPVITINSPAE
                     SAQFNPGATVNIMATISDIVGTKGMGMRSMLNGPKDKTGSGIDEVYLTVFDPNGVIVV
                     TDSTTAQVISKPLIVTEYGTYTINILAKDNVGNQAMANRNFIVTPATAPTISFTDIAW
                     LNSVGSNNLSFTINAVVPVNVTANVITYPSGATLMGPLSVNPEGGIYTVILNGGMIPA
                     GETSVRLQVIATDQFGNVTEANHYYGVDRSTPIITILNPVNGAEITLVDESTKVRIEA
                     QISDLIPPLKNGGKNSSGSGIAGSRMVIIDPLGMVISDVTAGADTTEISKEITNLMLG
                     TYTVRVTAWDNAGNQAMVSITFTMIAAPLPPAELAISDAYAYPNPSIDGTAKFAVTLT
                     NGAYVKIHIYDFAGREVRTLSSSGKVQGKSKAEIVFDGRNNDGVKLARGAYFARVIAN
                     DGTKIVEKVVKIAIK"
BASE COUNT         1993 a         1414 c         1150 g         2056 t
ORIGIN      
        1 caacctgaag atatggatta tccaattgcc tgtcaagtaa ctcctgttga ccaatacggt
       61 ttagcaggaa ctcctgtttt aagttctcct actattccta ttgaagattt gcctgcaccg
      121 cagaatctta cgggaacttt aactcctcct aatactgtta ccctgcattg ggaaaaacca
      181 attcattatc agggaaaggt cttcagcgga tataaaattt accgtgatga agcagttcta
      241 tacaacttaa tgaatcccga ttcactttcg tatgttgatt taaatgttcc tgatggcacg
      301 catacttatt gggtatgttc catttttaca tatcctctgg ttttttccag cccttccaat
      361 gcggttacga ttagcataaa tgtgcccaat agtgatgaga ttgctccagc tctgaaaagc
      421 gttagtgttt accctaatcc tttcaaaaca acaactaatt tccaaatttc cggcaaagct
      481 aatcgccctg taactattga aatctataac ttgaagggtc aactgataaa gcgtttggca
      541 accaaaaccg accaaaatgg tttagctcaa atcagctgga ttgctttaga tgaaaatgcc
      601 aagcccgtca gaaacgggat ttatttctac cgcacgattt gcgatagctc cagcgaaacc
      661 ggaaaattga tcctgctaaa atagcagcta acacaatttc ctgattcatt gtttagaatg
      721 cactcccaac ggcattgcgg ctgttgggag ttttttttat gttcgttcac accggaataa
      781 taatatccgg gaaacccggt ttgataaatt ctgctttacc aaacaaattg aaggtttcta
      841 aaggacttta tatctttttc atctttactg gcagttatcc tataaaattt taaatacttc
      901 cctgcatccg caaaatgata aggagttttt atccaaaaaa agaacaaact ctgttggcaa
      961 atctctcttt tgtagttttc ataatgagaa tgcataaata ttttcctgtc tgcagtgaat
     1021 ccctgccaag ttgaatcctt tatgcttctt taaaccttct ttaacgattc cttaacgaag
     1081 ttaaggtatt gttaaagaat cgttaaagag tcgttaaata aatgaccagg caacaggtag
     1141 ttaaagagag tccacaaggt aatttgccaa attaaaccga tgacgggagc atagctttta
     1201 tcttgtaatt tcattccgga aaaagctgct gccatcacac cttgtatctc ttttaatccg
     1261 tccaatctgt gccatctgtg agagacgata ctcaccccgt ttggtaaaaa aaagaatcct
     1321 gaagcaagct ccaggattct ttagtatagt taatttatca gagtgttatt tttccacagt
     1381 aaaggaataa atgcttctac cttgagattg aactttccaa cccaacatca tatcagcaat
     1441 tttttcagta gtttgttcct ccaggttacc caaaaggtca cgcacattgg cggactggat
     1501 tttgccgtta tccaggaaga gatcaacctt aatattgaat tgttttttat cagctagggt
     1561 ctgatgaatc atagttttaa actggttttc caaagaggca ggaattcctg tttgcgtaac
     1621 tgcatacata gaatccttgg aaatagtttt cacaacccga gcttttttct ctgcctgaga
     1681 aaattctttt tctatatctt cgtaggtatc ttccatatat tcaatgccca aaattttcat
     1741 ttcggttcgg cgatttcttt gtcttccttc gggtgtatca ttggaagcaa ttggcttatc
     1801 aaatccgtag ccaacggtct taatgcgttt gggatcaatg ccgtttttga ttaggtaatc
     1861 agcaacagct ttagcgcgtg cttcggaaag tttttgatta tattgtttac tgccaatatt
     1921 atctgtatgt cccgaaagct caatttccag ggtctcattt tctttcattg tagccaaaac
     1981 cggtttcagg atttccagag acctgggagt aagaactgcc ttattaaatt caaattctac
     2041 attctccagt tcaaagacag cttctttttc cagtttggat aaacggaagt ttttcgttat
     2101 attttgctga tcttcaggaa tatttatttc tccggaaacc tgaagataat tgggtttgga
     2161 aacacgatat tgcattttag gcatacgcgg taaagaaatg ctgtagtcac cgttactatc
     2221 agttacaacc cggtattcaa taatttcatt ttcactttca taaatccaag taaccatagc
     2281 tactaaagga ttatcattat cgtcaaagac cttaccgcta agtgtaactt ccttccccaa
     2341 tctaaccaaa cgaataattg tatcataact attcacattc ttgggaatag ttataatttc
     2401 ttcccgcact gcatagccgg gctcttcaat tctgtattta agcttttcca ttataggtgt
     2461 atagagttta aaaacacctt cattattgct ttctacaata acttcggtta attcgccctc
     2521 aaaaacatag ctccaacgaa tactgcaggg aacaggattg ttttcttcat ccagaacttt
     2581 accgttgata gcaagatttc caccttcacc aattcctgtt tctacgggaa gagtaatatt
     2641 tacaaatatc tcatttttat cgggtggaat ttcaatttta cctgttgttt tcttatatcc
     2701 gggagtattg atctcaaaag caatatcttt ggtgtccccg ggaagggtaa aagaaaaagt
     2761 tcctaattca tcagagccga taatacgcat aaatgtttta tcaccaagat cataaaccca
     2821 tataatatct gttttcacag gttgattttt atcatctaca actacgccgc tgattttcaa
     2881 ttctttaaac acttgaggaa taaccggttg ttcttcagtt ggcttaactc ccgtaagtaa
     2941 aatgatctgt tccttcatct tatccagtaa accaagatct attgtataaa tatcttcctg
     3001 ccccatacca ccggcacgat tggaacataa atatgcggtt ctgccatcag gagcaataac
     3061 aaaataacga tcatccttta cggaattaat tatcggaccc atatttaccg gtttgctcca
     3121 ttccgtccag gaattaccta ttcttgtgga aacaaagata tcattaccac cataactgtt
     3181 atgtccgaaa gaagcaaaat aaagatactt accatccgga ctcaaaaaag gcgattgttc
     3241 gtcataaggt gtattaacaa tatcacctac actaacagga gttgtccaga ttccgtttat
     3301 attttcggaa acataaatat catagttttt atgatctccg tcccgattac tggttaaaaa
     3361 cataacttta tcgcgccaga taaagggttg caggtcataa tatttactgg agacctcttt
     3421 gatcattagg ggtttggtcc agcgtccttt actatctaaa gttgtctgat aaatatctcc
     3481 attagttgta tctttggaat agtatccaaa taaataagca gtattgccat ccatagatag
     3541 agagccaaac gattcattat agtcggtgca aagttcatct acttcaattg cttcactgaa
     3601 tacattgttc tgctgctgag acagaaaaat attttcctta ccgaaattac tgcgtcggag
     3661 tgaactaaaa tacatatatt taccgtctgg agtaaagacc ggagcatatt cagaatcact
     3721 tgtattgatg ctgcctttca atttccccag ggacactgaa acagatgttt gttcttgata
     3781 cagagcaact tcataaagca tctgcagtaa atcttcctgc actaagggat ctgctcccaa
     3841 tgattttttt ttgtattcag atagccaaat atgcgcttgt ttgtaatccc ctaaagccag
     3901 atggactttc ccgcacaaca aagcatattc aggatcctca ttatcaggtg cctgaatcat
     3961 atccaaattt tgtttcgcgg aagcataatt acccaattca taatattgcc gggcattaat
     4021 aaaatagatg ttatcattca aggcgagagc aaactggcaa gacaggagga ttatcaacca
     4081 gattattagt ttcttcatta tatctcctct taatgtgctg gctcccccct gagaaaaaat
     4141 cctcagggga aaaccacaca caattttttt attgcatacc agatatccca gttacaagcg
     4201 ttttaaaaac gcagtaacag ggaaactgaa tgagtatcat tcaagtattc gtcatgtgcc
     4261 atctggaatg cataattcat ttctaacatc ttaaaccgca gtcctgcacc agctgtaaaa
     4321 gcaccatcat taacaccggc acgaattcca gcttgaactt tggataagat atcagaccag
     4381 gttgcactct ctctaatacg aattccggat aaactggagc caatttcagc atcatctttt
     4441 acgccgagcc aatattcaac tcccaaacgc aattttgact cctcaaaatt atcacgaccg
     4501 gaaaggtctg cggcaataat caaacccggt attggataag cagcaactcc aggaatgaac
     4561 tctataggaa tgtcctcttc ttctccagga taattggcta ttaaatccct aactaccatt
     4621 cctacattaa aatagcgatt gatatgatag agaaatccta aatccactcc ataaccggta
     4681 tcggattcat tatcctgatt ggagagatag aacttgggcg taatgcctaa attcagtttg
     4741 gagattttgg cagcataact taatgcaagc gtgttatcac tattgctaaa atcccctgta
     4801 gggttgccat taacatcgta tcctggaata tcactaacag tagcattttg ccaggatgct
     4861 gcaacataac ctatgggcaa aacaaaccct aaagcaactg cattatgatt tctatcccaa
     4921 gccatattct gtcttgtgcc tgaagtaaat tctatcgttt taacatctgc caaaactgcc
     4981 ggatttaggt atgcgcttga gacattatcc aaaaacgcgg ttccagttcc acccatacca
     5041 ttagaacggg catctatacc gagcaattga tatactgctg cagaattatc ttcagcaaag
     5101 acagaagaca gtgaaatcat cagtatcagg gctatcagta ctaatttaat ttgtttcatt
     5161 tttttccttc ctttcggttg gaagggaggg ggagccccct cccctcttca ccaatttact
     5221 tgatagctat cttaactacc ttttctacta tcttcgtgcc gtcgttggca atcacacggg
     5281 caaaatatgc tccgcgcgct agttttacac catcattatt tcgaccgtca aaaacgatct
     5341 ctgctttact cttgccctgc actttaccac ttgaacttaa agtgcgaact tcacgacccg
     5401 caaagtcata aatgtggatc tttacataag caccattggt tagggtaacg gcaaatttgg
     5461 cagttccatc aatactagga ttgggataag cataagcatc agaaattgcc agttcagcag
     5521 gcggtagagg agcagcaatc attgtgaaag taattgatac cattgcttgg ttacctgcat
     5581 tatcccatgc agttacacgc acagtgtaag ttccaagcat cagatttgtt atttctttgg
     5641 aaatttcagt agtgtcagca cctgctgtaa catcactaat taccatacca agaggatcaa
     5701 taatcaccat acggcttcca gcaattcctg aaccggaact gtttttaccg ccattcttca
     5761 gaggtggaat cagatcactg atttgggctt caattctaac tttagtggac tcatccacca
     5821 aagtaatttc cgcaccattt acaggattaa gaatagtgat tataggagta gaacgatcca
     5881 caccataata gtgattagct tcagttacat taccaaattg atcagtagca attacttgca
     5941 agcgaacaga ggtctcaccc gcaggaatca taccaccatt caatattaca gtgtaaatac
     6001 caccttcagg atttacggaa agcggaccca ttagggttgc tcccgaagga taagtaatta
     6061 cattagcggt aacattaaca ggaactactg cattaattgt gaagctaagg ttattagaac
     6121 ctacagaatt caaccaggca atatcggtga agctaatcgt aggagcagta gctggtgtaa
     6181 caataaagtt acgatttgcc attgcttgat ttcctacatt atcttttgcc agaatattga
     6241 tagtgtaagt tccatattca gtaactataa ggggtttgga aatgacctga gcggttgttg
     6301 aatctgtaac aacaattaca ccattaggat caaacacagt tagataaact tcatctattc
     6361 ccgaacctgt cttatcttta ggaccattca acatgctacg cattcccata cccttggtgc
     6421 ctactatgtc agaaattgta gccataatat ttactgtagc accaggattg aattgcgcac
     6481 tctctgcagg actgttaatt gtaattacag gtgcactgtt atcaatacca tagacctgat
     6541 tggaagtaga agttccatcc agaatattag tagcagtcac ttccaaacga attccggtct
     6601 gatctacagg tat
//
LOCUS       DAOOZV010000080         6329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_10834_length_6329_cov_55.123207, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000080 DAOOZV010000000
VERSION     DAOOZV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_6329_cov_55.123207"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1291
                     /gene="pgsB"
                     /locus_tag="PLW08_07030"
     CDS             101..1291
                     /gene="pgsB"
                     /locus_tag="PLW08_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPL52390.1"
                     /translation="MTVLIIVTLILITHWIIEYQRHTKNVLSIPIRIHINGTRGKSSV
                     TRLIAAGLREGGKVTVAKTTGTLPRVILPDGREAAILRLMGANIIEQKYIFRHAVKHK
                     PDAIVIECMAVNPVLQWITERKFIRSTISVITNCREDHLDLMGSTVQSVTMCISNTIP
                     KKGICYTAEEEQYPLLEKVAKSRQCQIHKIRPVDVTEEELNKFRYIEHSENVQLALAV
                     CAAAGVPRDVALRGMQKATPDPGALKKYPIKDRGKTIIFYNVFAANDPQSTVEIIQMV
                     TGNLQNIEKIIILNSRADRLFRSHQLVDAIKPLDFSYLLLTGEIPDKIENYAIQAGIP
                     KEKIFPLGEPLPEVIYQKVWELTKDESHILGIGNIAGTIKYGAQIVAHFRHKMKECNE
                     RSRM"
     gene            1288..1743
                     /gene="pgsC"
                     /locus_tag="PLW08_07035"
     CDS             1288..1743
                     /gene="pgsC"
                     /locus_tag="PLW08_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HPL52391.1"
                     /translation="MIDTIVQAAVGMGVIISLMFSELLGASAGGIVVPGYIALYLDRP
                     LQILGTLVISLLTWAIIRIIGNFTLLFGKRRMVLSILIGFILGWLSRILVVQDITIHQ
                     LQMQSIGYIIPGLIANWFERQGFWKTVSSMGIAAIVVRLALMVIFNGEL"
     gene            1746..2870
                     /gene="pgsW"
                     /locus_tag="PLW08_07040"
     CDS             1746..2870
                     /gene="pgsW"
                     /locus_tag="PLW08_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPL52392.1"
                     /translation="MMYRPSLKSNRSLIILLLLAIVLFYIAQTSYVNVKTDNYEIKIA
                     AAQLMKSAIDSLSAEINKRGIEIDPIDDPLQTGLIGMRLSSITTDRGLLSEKRAAINP
                     NLAAVFVEELSKMKLMPGDYIAVGITGSNPAVNIALYSAIQVMELNPKIIVALSSASY
                     GANRPELTWLDMESILKEKGLFDFGSSYASFGGKDDLAIGLSDNGIKALREAMQRNNV
                     PLLIGNDLEDNIAIRMAAYEELLPEGKRYKAFVNIGAGLANVGSEPNANLIPEGINRR
                     LAEHNYEKEGVIIKMAKNNVPVLHFRRILRWAKRYNLPTSFDTMPKVGEGKMFGSRIH
                     NQTVNIICLTILLIAIVIVIIFDRHDRRFMANIVDLDEEL"
     gene            2883..3704
                     /locus_tag="PLW08_07045"
     CDS             2883..3704
                     /locus_tag="PLW08_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52393.1"
                     /translation="MHKHFISVLLIILGINLLLAQTVNRYKVLPFDNPGTKLLLKTEA
                     GNYYYYRGLPERPLILNTTGVEKIELRSFSKEAVRKPEIVIVINKQRKTYPLSFKEKK
                     GNYYLYQPLMIDIPENTKEIQIICYDRSIYMRAFSVLPPKPPKAAKQPNIAIKAHSGT
                     VSLQHNGSNSDYYTFMPNQPFKFTLNNGRNAYVYVRPRLLDCSLPKIGLYANGELVEV
                     IDFTLKRTTKYSCQGISSLGIAKKIVLPANEKSTDYELRALSDHLFLAKPILMKK"
     gene            3714..4865
                     /locus_tag="PLW08_07050"
     CDS             3714..4865
                     /locus_tag="PLW08_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52394.1"
                     /translation="MADTNPKIFPQEAKPIAELKEKETIASLDSLSPKQTKENKTVKK
                     SKIRRKNSIRPIILLLTFFLFFGFSQVEAQINGSVKIGAVYSDNVFHLSEYDIDRFDD
                     HHPNLNFVDTIDDLTLNTRIELKYPLSYRWWKFTPSVSGNFSQNISNEDKYRTDWTLK
                     FKVDRYYWNCSVQYTSNPYIYFRHFVDTDGTGELEKYSYSRDTYRGDLAVKPLPKTTV
                     KTNLRYELYRYNKYFTEADGKAITGEAGVSYKFPFFTVEGSYGYRDFSCDKRIANKDC
                     SYQSNIYKGALTLPKMPLSEKGKTLIQPSFALNYEQRFYQGSGSWYGGRADYTYSLMA
                     GMELFFSPKMNLSLDYSHYFRNVETDNETVQRLKEYSENRLSAVLSYKF"
     gene            4881..5213
                     /locus_tag="PLW08_07055"
     CDS             4881..5213
                     /locus_tag="PLW08_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52395.1"
                     /translation="MVFKKENDLRKIKEFLTLVENPKVELRHYKKPVLVWAVDEGTVF
                     YSFWEEEMLTRFGLENVDGWDFQEDLLFCPDWIGDTEEEDLEDFDLDDEDIEELSHFL
                     HRNMDDKD"
     gene            5216..6223
                     /locus_tag="PLW08_07060"
     CDS             5216..6223
                     /locus_tag="PLW08_07060"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPL52396.1"
                     /translation="MRIATLGGGGWGLALSSLLSENGHQILVWEYNPEYLILLKETHS
                     NPHLLPNITLPEEVCFTNDFQEIVAFSPEIIILATPSQFLRSTLQKTPVSLWNRPELL
                     AVVNVAKGIEENTLKTLDAVIKDELPFLEEHKICALSGPSHAEEVARKIPTTVVIAGS
                     DEDLLCYLQTVFSNSYFRVYRNLDLIGVEIGGAVKNIIAIAAGIIYGLDFGDNTIGAL
                     LTRGIVEIQRLGIALKALPETFLGLSGIGDLITTATSLHSRNRFVGVEIGKGRKLSDI
                     LAQMEMVAEGVTTTRSVYLLSRKLRIEMPIVEKVYQVLYEDKEPRKAILELMTRELKA
                     E"
BASE COUNT         2033 a         1103 c         1296 g         1897 t
ORIGIN      
        1 ataaatggaa tgttaattgc atttataaag atagtcactt tttgcttagc caagtgcaaa
       61 gctggaagtg gatgtaatat aattactaac agaggaatag atgactgttc tgataatagt
      121 gactttgata ctgataacgc attggattat agaatatcaa aggcatacaa aaaatgttct
      181 gtcaattccc attcggattc atattaacgg gacacggggg aaatcaagtg ttactcgtct
      241 tatagctgcc ggcttaagag aaggggggaa agtaacggta gccaagacca cgggaacctt
      301 acccagagtt attcttccgg atggaagaga agcagctatc ttgcgtttga tgggggcgaa
      361 tattattgaa cagaaatata tcttccgtca tgctgtaaag cacaaaccgg atgccatagt
      421 tattgaatgt atggcagtaa atccggtttt acagtggata acggagcgta aatttattcg
      481 gagtactatt agtgttatta ccaattgtcg ggaagaccat ctggatttaa tgggttctac
      541 tgtgcaaagc gtaactatgt gcattagtaa tactataccg aaaaagggaa tatgttacac
      601 agcagaagaa gaacaatatc cccttttgga aaaagtggca aaaagcagac aatgtcaaat
      661 tcataagatt cgtcctgtag atgttacgga agaggaattg aataaattca gatatattga
      721 gcatagtgaa aatgtccaat tagcgttagc tgtatgtgct gcagcgggag ttccccgaga
      781 tgtagcttta aggggaatgc aaaaagcgac acctgatcct ggtgccttaa aaaaataccc
      841 aattaaagat agaggcaaaa caataatatt ctataatgta tttgctgcaa acgatcctca
      901 gtcaacagta gaaatcattc aaatggttac cggcaatttg caaaatatag aaaagattat
      961 cattttgaat agcagagcag accgcctttt tcgtagtcat caattagtgg atgcgatcaa
     1021 gcccttggat ttttcctatt tattgctaac gggagaaata cctgataaga tagaaaacta
     1081 tgctattcaa gcaggtatcc ctaaagaaaa aatatttcct ttaggtgaac ccttaccgga
     1141 agttatctat caaaaagtgt gggaacttac taaggatgaa tctcatattt tgggaatcgg
     1201 taatattgcc ggaacaataa aatatggggc acaaatagta gcccacttca gacataaaat
     1261 gaaagagtgt aatgaaagga gccggatgtg attgatacaa tagttcaagc cgcggttggg
     1321 atgggtgtga ttatttcact gatgttcagt gaattacttg gtgcttctgc gggaggaata
     1381 gtagttccag gttatattgc tttatatttg gatagacctt tgcagatttt aggcacttta
     1441 gtaatcagtt tattaacctg ggcaatcata cgcattatcg gtaattttac cttgcttttc
     1501 ggtaaacgga ggatggtgct aagtatttta attggcttta ttttgggttg gctttcccgt
     1561 attttggtag ttcaagatat tacaattcat caattgcaga tgcaatccat tggttacatc
     1621 attcccgggc ttattgctaa ctggtttgaa cgccagggtt tttggaaaac agtatcttct
     1681 atggggatag ctgcaatagt tgttcgtttg gctttaatgg ttatatttaa cggagaattg
     1741 taaaaatgat gtatcgtcct tcactcaaat ccaatcgttc gttaatcatt cttcttttat
     1801 tagccattgt cttgttttat atagcgcaga ccagttatgt taatgtaaaa acggataatt
     1861 atgaaatcaa aatagctgct gcacagctga tgaaatcggc aattgattcc ttatctgctg
     1921 aaataaataa aagaggaata gaaatagacc ctatagatga ccctctgcaa accggtttaa
     1981 tcggaatgcg tttaagttct ataactacgg ataggggttt gctttcggaa aaaagagctg
     2041 ctattaatcc caatttagcg gctgtatttg tagaagaact atccaagatg aaacttatgc
     2101 ccggggatta tattgcagta ggaattacgg gaagcaatcc agcagttaat atagctcttt
     2161 attcagccat ccaggtaatg gagctgaatc ctaaaataat agttgcccta tcttccgcat
     2221 cttatggagc aaaccgtccg gaacttacct ggctggatat ggaaagcatt ctgaaagaaa
     2281 aaggtctctt tgattttggc tccagttatg cctcttttgg aggaaaggat gacctggcaa
     2341 ttggcttatc ggataatgga attaaagccc ttcgggaagc aatgcaaaga aataatgttc
     2401 cgcttttgat cggtaatgat cttgaagata acatagctat ccgaatggca gcttatgaag
     2461 aactattgcc ggaaggaaaa cgctataaag catttgtgaa tattggagct ggtttggcta
     2521 atgtaggcag cgaacccaat gctaatctta ttcctgaagg aattaatcgc agattagctg
     2581 aacataatta cgaaaaagag ggcgtgatta ttaaaatggc aaaaaataat gttcccgtct
     2641 tgcattttcg ccgtatttta cgctgggcaa aacgctataa ccttccgact tcttttgaca
     2701 cgatgcctaa agttggtgag ggaaaaatgt tcggttcccg catccataat caaaccgtca
     2761 atatcatttg tttaactatc ttacttattg ccatagtaat tgttattatt tttgaccgtc
     2821 acgaccgtcg gtttatggct aatattgtag accttgatga agaactttaa ggaaactaaa
     2881 aaatgcataa acattttata tccgtcttgc taatcattct gggaatcaat ttgcttttgg
     2941 cacaaacagt aaacaggtat aaagtcctgc cttttgataa tccaggcaca aaacttctgc
     3001 tcaaaaccga agcaggaaac tattattatt atcgcggttt gccggaaaga cctctaattt
     3061 taaatactac gggtgtagaa aaaattgaac tccgtagttt cagtaaggaa gcagttcgta
     3121 aaccggaaat tgtaatcgtt attaataaac agagaaagac ctatccactt tcttttaagg
     3181 aaaagaaggg aaactattat ctctatcaac ctcttatgat agatattccg gaaaacacta
     3241 aggaaattca gataatatgc tatgaccgtt caatttatat gcgcgctttc agtgttttac
     3301 ctccaaaacc ccctaaggca gcaaaacaac ctaacatagc tattaaagct catagcggaa
     3361 cagtaagttt acaacataac ggctccaaca gcgattatta cacttttatg cccaatcaac
     3421 cctttaaatt tactcttaat aatggcagaa acgcctatgt ttatgtgcgt ccgcgtttgc
     3481 ttgactgttc tttgcctaaa ataggattat atgccaacgg ggaattagtg gaagttattg
     3541 attttacact caaacgcacg acaaaataca gctgccaggg aattagcagt ttgggaattg
     3601 ccaaaaaaat tgttttaccg gcaaatgaaa aaagtactga ttatgagctg cgcgctttga
     3661 gtgaccatct gtttttggct aaacctattt taatgaagaa gtagagctgt tttatggcag
     3721 atacaaatcc gaaaatattt cctcaagaag caaagcccat agcggaatta aaagaaaaag
     3781 aaactatagc ttcccttgat tctctttccc ccaagcaaac aaaggaaaat aagaccgtta
     3841 aaaaaagcaa gataagaaga aaaaacagta ttcgtcccat aatccttctt cttacttttt
     3901 tccttttttt cggcttttcc caagttgaag cgcaaataaa cggttcagtt aaaattggag
     3961 ctgtttattc ggataatgtg tttcatcttt cggaatatga tattgaccgg tttgatgacc
     4021 atcatccaaa tctaaatttt gtagatacta ttgatgattt aaccctaaat acccgcattg
     4081 aacttaagta tccgttatct tatcgctggt ggaaatttac tccttcagta agcggaaatt
     4141 tttcccaaaa tattagtaat gaagataaat accgcactga ctggacatta aaatttaaag
     4201 ttgaccgcta ttactggaat tgcagtgtgc aatatacaag caatccctat atttatttcc
     4261 gtcattttgt agatacggat ggaacaggtg aactggaaaa atacagttac agcagagata
     4321 cttatcgcgg tgatttagct gtgaagcccc ttccgaaaac aactgtaaaa actaatctgc
     4381 gctatgaact ttatcgctat aataaatatt ttacggaagc tgacggcaaa gccataaccg
     4441 gagaagcagg tgtcagctac aaatttccct tttttacagt agagggttct tatggctatc
     4501 gggatttcag ctgtgataaa cgcatagcta ataaggattg ctcctatcaa agcaatattt
     4561 ataaaggagc tttaactctg cccaaaatgc ctctttccga aaaaggaaaa acccttattc
     4621 aaccttcttt tgctctaaat tatgaacagc gtttttacca gggaagcggt tcctggtatg
     4681 gaggcagagc tgattatacc tattctttaa tggctggtat ggagctgttt ttttctccta
     4741 aaatgaattt atctcttgac tactctcact atttcaggaa tgtagaaaca gataacgaaa
     4801 cggtgcaacg cttgaaagaa tatagtgaaa atcgtttaag cgctgtttta agttacaaat
     4861 tctgaattta gaggtgataa atggttttta aaaaagagaa tgatctccga aaaattaagg
     4921 aattccttac tttagtggaa aatcccaaag tggaattacg ccactataaa aaaccggttt
     4981 tagtttgggc tgttgacgaa ggaactgtct tttattcttt ttgggaagaa gagatgctca
     5041 cccgttttgg attggaaaat gtggacggct gggatttcca agaagacctt cttttttgtc
     5101 ccgactggat tggagatacc gaagaagagg atttagagga ttttgatttg gacgatgaag
     5161 atattgaaga actgagccat ttccttcatc gcaatatgga tgataaggat taactttgcg
     5221 cattgccact cttggaggtg ggggatgggg gttagcatta tcttctctgc tttccgaaaa
     5281 cgggcatcaa attctggttt gggaatataa tcctgaatat ttaatattat taaaagaaac
     5341 acattctaat ccgcatttac tgccgaatat tacgcttccg gaagaagttt gtttcactaa
     5401 cgattttcag gagatagttg ctttttctcc ggaaattatt attttagcaa ctccttcgca
     5461 gtttctacgc tccactttac aaaagacacc tgtttcttta tggaatagac ctgaactttt
     5521 agcagtggta aatgtggcta agggtattga ggaaaatacc ttaaagacac ttgatgccgt
     5581 tatcaaagat gaactgcctt ttctggaaga gcacaaaatt tgtgccttat ccggtccctc
     5641 ccatgccgaa gaagttgcca gaaaaatacc tactactgta gttattgccg gtagcgatga
     5701 ggatttgctc tgctatttgc aaaccgtttt tagtaattct tatttccggg tttatcgcaa
     5761 tttagacctt attggagtgg aaatcggcgg tgcagtcaaa aatattattg ctattgcagc
     5821 aggaattatt tacggattgg actttggaga taacactatc ggagctttat taacccgagg
     5881 aattgtggaa atacaacgcc tgggaattgc tctgaaagct cttccggaaa catttttagg
     5941 actttcagga ataggggact tgattactac agccacttca cttcatagca gaaaccgctt
     6001 tgtaggcgtg gaaataggaa agggaagaaa actttccgat attttagcgc aaatggaaat
     6061 ggtggctgaa ggagtaacaa ccacacggag cgtttatttg ctgtcccgaa aattaaggat
     6121 agaaatgccc atcgtagaaa aggtctatca agtcctttac gaagataaag aaccccgaaa
     6181 agcaatctta gaactgatga cccgcgaact taaagccgaa taaaaatcca cagatgaagg
     6241 aaaaatagtt ttatttcacg ctgattccgc agatctcgca gattttattt ttacaaagag
     6301 aaaagagaga aagagtaaga atgaaaaat
//
LOCUS       DAOOZV010000081         6317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_10853_length_6317_cov_66.457522, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000081 DAOOZV010000000
VERSION     DAOOZV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10853_length_6317_cov_66.457522"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..2746)
                     /locus_tag="PLW08_07065"
     CDS             complement(293..2746)
                     /locus_tag="PLW08_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL52397.1"
                     /translation="MILTDAQLFSEISRCEFCENKPCKNACPCDCSPADFMMAAAKGR
                     PSDFKRSAGLILASNPLGGICGNVCPDDHCVNACARENFDTPIQIPAVQATIIRKARE
                     LNMVPEFTPPFSLNKKIAIIGAGPSAIGAAVTLTQFGYQTVMFDPEPLGGQVNLIPEE
                     RLEKIDLLDDLKFLAETFKIEHIRKKIEEPQTLLNDDFEAVIVAGGLNKPIHLNIPGD
                     ETAIYGFDILKNPSQYNFTGKRVALVGGAIAADMALLIANNNAAYVEMITLENFTEMP
                     LTEPEKATLIEAGIEFSPRTRITEIVNEGNQIKGIKTKKVYLPKGEKFAPNLIRDEAG
                     TTESFRPFDIVIIAIGNKPPFDVNKLETLSDRLFCCGDMINGPTTVVEAVASGKNTAL
                     KVHSLLSKKDISEPEKATKSCFPVWGWKKYPVSLETDFFGRKMDSPFILSAAPPSDGY
                     EQMKKAYLAGWSGGIMKTAFDNEDIHIPGEYMFTFGDKKKTFANCDNVSEHPLDRVCS
                     EIALLIKEFPERLTMASTGGPVTGDDINDKKGWQANTIKLEKAGAMGIEYSLSCPQGG
                     DGTKGDIVAQDPALTAKIIDWVMEIGNPEIPKLFKLTGAVTSIYPILSAVKSVLDKYP
                     GKKAGITLANTFPALAFREKEKPWDEGIIVGLSGEGILPISYLNLARAGNSGIFISGN
                     GGAMNYFDAANFLALGAGNVQFCTIVEKYGYGIIEELKMGLSYLLEAKGLKSVAELIG
                     IAQPKPITDFLDLPTKTKSSSLIKELCLKCGNCTRCPYQAIELDEDCYPKIDPDKCVG
                     CSLCTQLCFAGALEMKNEK"
     gene            complement(2949..4490)
                     /locus_tag="PLW08_07070"
     CDS             complement(2949..4490)
                     /locus_tag="PLW08_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPL52398.1"
                     /translation="MLKVSIAPDGKEYPLPSEEDAQKEREILKQITAEQKALGRKIVA
                     VQGLGFVGCVMATVVADATDKDGNPLYFVHGHQRASQRSYWKVPVINSGIPPVNSSDP
                     EVPAIFHRTVVEKKNFRATSEESVYGLVDIVVVDIQLDATKPAFGDAEKGYCDITAFR
                     EGIRTLGKHIQPDCLVLVETTVPPGTCEKVVKPILEEEFTKRGIDIEKNPPLIAHSYE
                     RVMPGAHYVASIRDFWRVFSGVNPKSIELCQEFLSNVLDVENYPLTQLDSTNASELAK
                     TMENSYRAVNIALTLEWAKFAEQIGVDIFKVRDAIRKRKGTHDNLLRPSLGVGGYCLT
                     KDPVLANWAMKSLFGVEGSLDFAIKAVNTNDTMPLHTIDIVKSVYPDLKNLKIAVLGV
                     SYLEDVGDTRHSPSKTLVEFLRKDLAIVKTHDPYVETWPEMEENHIQNDLNNVLPESD
                     VVIFAVGHSQYRKLDPKYVVDLCKTKPLIVDCSNFLSDETINIYKDLGCKVRGVGKGH
                     IVNEK"
     gene            6091..6258
                     /locus_tag="PLW08_07075"
     CDS             6091..6258
                     /locus_tag="PLW08_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52399.1"
                     /translation="MKPQKRKTKFILVLHQGNLPNSHCILSELNGNIPFFNGILPQTK
                     EINLLKKARCK"
BASE COUNT         1925 a         1278 c         1104 g         2010 t
ORIGIN      
        1 taatataatc cagtaactcc tttctatcct gttatctgca ttctatcatt cattcaccgt
       61 tctcaattct caattttacg aggggcttac gcacccatcg ctatcattgt gtcgcccttt
      121 gggctaaaaa agaattaaaa aaaatctcaa ccttctcaac cttctcaacc ctctcaacct
      181 ttttttacga ggggcttacg cacccatcgc tatcattgtg tcgcccttcg ggcttttagc
      241 aaagagcatt gagtttttac cgttttttac tacattttac attctacatt tttcattttt
      301 cattcttcat ttccagcgct ccggcaaagc acaattgtgt acacagtgag catccaacac
      361 atttatccgg gtcaatttta ggatagcaat cctcatctaa ttctattgcc tgataaggac
      421 agcgagtgca gtttccgcat tttaggcaca attctttaat taaagaagag ctttttgtct
      481 ttgtgggcag atcaagaaag tccgtaatgg gttttggctg ggcgattcca ataagttcgg
      541 caacgctttt taagcccttt gcttccaaga ggtaagaaag tcccattttc agttcttcta
      601 taatgccgta gccatatttt tccacaattg tgcagaattg cacatttcct gctcccaggg
      661 ctaagaaatt ggcagcatca aaataattca tagcacctcc gtttccggag atgaaaattc
      721 cgctatttcc agctcgggct aaattcaaat aactgattgg caaaatgcct tcaccgctta
      781 aaccaactat gattccttcg tcccagggtt tttctttttc tctgaaagcc aaagcaggaa
      841 aggtattagc caaagttatt ccggcttttt taccaggata tttgtccaaa acacttttaa
      901 cagcggaaag aataggatag atagaagtta cagcaccggt tagtttaaac agtttgggta
      961 tttcgggatt gccgatttcc atcacccaat ctataatttt agcggttaaa gcagggtctt
     1021 gagccacaat atcacctttt gttccatctc caccctgagg acaggataaa ctgtattcaa
     1081 ttcccattgc gcctgctttt tccagtttta tagtattggc ttgccagccc tttttatcat
     1141 taatatcatc accggtaaca ggaccgcctg ttgaagccat agttaagcgc tcgggaaatt
     1201 cctttatcaa aagagctatt tcggaacaaa ctctatccaa aggatgctct gaaacattat
     1261 cacaattggc aaaggtcttt ttcttatcac caaaggtgaa catatattca cccggaatat
     1321 gaatatcttc gttatcaaaa gctgttttca taattccacc gctccagcca gcaagatatg
     1381 cttttttcat ctgttcatag ccatcggaag gaggagctgc agaaagaata aaaggggaat
     1441 ccatttttct cccaaagaaa tcggtttcaa gtgaaacagg atattttttc cagccccaaa
     1501 cagggaaaca gcttttagtt gctttttcgg gttcactaat atcctttttg cttaatagag
     1561 aatgaacttt taaagccgtg tttttacctg aggcaactgc ttccaccaca gttgtaggac
     1621 catttatcat atctccacag caaaaaagac ggtctgaaag ggtctctaat ttattaacat
     1681 caaaaggagg tttgttgccg atagcaataa taacgatatc aaaaggacga aaggattctg
     1741 tggttcctgc ttcgtcccta atcaaatttg gagcaaactt ctctccttta ggaagataaa
     1801 cctttttcgt ctttattcct ttaatctgat taccttcatt aactatttcc gtgatcctgg
     1861 tgcgaggcga gaattcaatt cctgcctcaa tcaaagttgc cttttccggt tccgttaagg
     1921 gcatttcagt gaaattttcc agggttatca tttccacata agcagcatta ttattagcaa
     1981 tcagcaatgc catatcagca gcgattgctc cacctacaag agcaacgcgt ttaccagtaa
     2041 aattatattg ggaaggattt tttagaatgt caaaaccata aatagctgtc tcatcaccag
     2101 gaatatttaa gtgaatgggc ttatttaaac cccctgccac aattaccgct tcaaagtcat
     2161 catttaataa tgtttggggt tcttctattt ttttccgaat atgttctatt ttgaatgttt
     2221 ctgccaggaa tttcaggtca tccagcaagt caattttctc caaacgctct tccggaatta
     2281 aatttacctg acctcctaaa ggttctggat caaacattac cgtctgataa ccgaattggg
     2341 ttaaagttac ggcagctccg atagcagaag gtcctgcacc gataatggca attttcttat
     2401 ttaaggaaaa gggaggagta aattctggca ccatatttaa ttctctggct ttacgaatta
     2461 ttgttgcctg aacggcagga atttgaattg gagtgtcaaa attttcacgc gcacaggcat
     2521 ttacgcaatg gtcatcggga caaacatttc cgcaaattcc ccctaaagga ttgctggcta
     2581 aaatgagtcc tgcagatctc ttaaaatcgg aaggtctgcc tttagcagct gccatcataa
     2641 aatcggcagg agaacaatca cagggacagg catttttgca gggcttgttc tcacaaaatt
     2701 cacaacgact tatttcactg aataattggg catcagttaa aatcatttat cctccaattt
     2761 tcatagccac cgagagcata gaagtcaccg aaaaagatag gagcacgcag atgtctctca
     2821 cagatttcac agatatttca ttttatagtt actttcctaa tatactccag taaatccatt
     2881 ctatccagtt atctatgttc tatcattcat tcaccattct caattctaca tttaacattc
     2941 tgcattgttc atttttcatt cacaatatgt ccttttccca caccccgcac tttgcatcct
     3001 aaatctttat atatattgat ggtttcatcg gaaaggaaat tggagcaatc cacaattaag
     3061 ggtttggttt tacataaatc aaccacatac ttagggtcaa gttttctata ttgactgtgt
     3121 cctaccgcaa aaataactac atcggattcg ggaagaacat tattcaggtc attttgaata
     3181 tggttttcct ccatttcggg ccaggtttct acataaggat cgtgggtttt cactattgcc
     3241 aggtctttcc gcaaaaattc cactaaggtt ttggaaggag agtgtcttgt atcacctaca
     3301 tcttccaaat aggaaacgcc taaaacagca atcttcaagt tttttaaatc aggataaacg
     3361 cttttaacga tatcaattgt atgtagcggc attgtatcat tcgtattaac tgctttaata
     3421 gcaaaatcca aacttccctc tacaccaaaa agtgacttca ttgcccaatt agctaaaacg
     3481 gggtctttgg ttaaacaata accgccaaca cctaatgaag gacgcaacaa attatcatgc
     3541 gttccttttc ttttgcgaat ggcatcgcgc actttgaaaa tatccacccc aatctgttcc
     3601 gcaaatttag cccattccaa agtgagagca atatttacgg cacgatatga attttccatt
     3661 gttttggcaa gttcgctggc atttgtgctg tccaattgag ttaaaggata attctccaca
     3721 tccaaaacat tggaaagaaa ttcctggcag agttctatac ttttagggtt aacaccggaa
     3781 aaaacacgcc agaaatcacg aatagaagcc acataatgag ctccgggcat aactctttca
     3841 taagaatgag caatcaaggg aggattcttt tctatatcta taccccgctt ggtaaattcc
     3901 tcttccagaa tgggcttaac gaccttttca caggttccag gaggaacggt tgtttctact
     3961 aaaactaaac agtcgggctg aatatgtttt cccaaagtcc gaattccttc ccgaaaagcg
     4021 gttatatcac aataaccttt ttctgcatct ccaaaagcgg gttttgtagc atccaactga
     4081 atatctacca caacaatatc tactaaacca taaacacttt cttcactggt agcacggaag
     4141 ttttttttct ccactacggt gcgatgaaat attgccggaa cttcgggatc ggaagaattt
     4201 acaggcggaa tgccactatt gataaccgga actttccaat acgatctttg tgaagcacgc
     4261 tgatgaccat gaacaaaata taaagggttg ccatctttat ctgtagcatc agcaacaaca
     4321 gtagccatca cacaacctac aaatcccaaa ccttgaactg ctactatttt tcgtcctaaa
     4381 gctttttgtt cggcagtaat ttgttttaat atttctcttt ctttttgtgc atcttcttca
     4441 ctcggaagag gatattcttt tccatctggg gcaattgaaa cttttaacat attttttgtt
     4501 aactccttta tatatataaa gtaatgatac tggaaaaact aaatcctgta gtttattggt
     4561 tgatgagaac atcaacctta cgggaaaacg acattctatc gttcatttat aacttttcca
     4621 tttccaaatc gtttttacgc tttttatagg gcttttattg tcaagatgtt tttcagaaag
     4681 cagagggcag agggcagagg gaagagagca gagggctgag ggcagagggc tatgaggaaa
     4741 agaatatatt aattttattg aacaaggggc ttacattacc atcggtgaag gagttttgac
     4801 ttacaatttt gctattgcca tcaaagaaac cactttcctc aattttatac ttatccaacc
     4861 gagaaataaa atgattccga ttttccaact tgtgagttaa aacagaacta acttcttgtt
     4921 aaatagtcaa tagcgtaacg actccgtaac acttccgtaa cacttccgtt gtctcgttac
     4981 ggaattgtta cttggttgtt actttgttat aacttgctga agggtgcgga gtgcgtcctt
     5041 caattggaaa tttttttgcc cgaataaaag cttttgggca tttttgtcac cactttgtat
     5101 tttttccagt atatatacta tagaaggata aaaaagttga gatggttttg aaggttgagt
     5161 aaaataattt ctttccaagc tctaagaatg agacaacgat aataaagaaa aaggcaaaaa
     5221 ctaaaaacgc attcttttgt tgaagtagaa aataactgtt aattttgaat ctaatattgt
     5281 ttaaacaaaa ttctcaaaaa tatcccgttg ttctcagttt cctatagcgc tctcagtgtt
     5341 aaacaaaatt caacgaaaaa gttgttgcct aatcttcata atggattagt aatattggaa
     5401 tgcgggaaaa aagtggtaga agagaaaatg ttgttttatt aaagcgaaaa agatttgcca
     5461 aatagtaatt gtaacatctt gaaaatatta tcagttacaa aatatgcgct tttagcagaa
     5521 aaaatttctt gacaaataat ctgcgtgctg tttactggat atagagttgg agttttcctt
     5581 ggctctgaaa ttcagaaaag taggaaaaag gaatgcgaag ttttattttt tttacgagtg
     5641 caaatgcagt tcagcaaaag aaactgatac tgccaaaaag tagatttaag cgttagctat
     5701 cagttttaaa tgaaattgga aattatgttt ttcatatttt tttgtcgctt atcagctgag
     5761 gtggtaaagc aacatctatg agacccgcag cttttagttt cgtaaaaagg ggacttgtta
     5821 attttaaaat gaaaatgaat gtttggtcta caatctggtt gctgctaaca gatagttctg
     5881 tattggcttc cgggaaaaca gttgcaaaat ttttaaccgg gctttattcc cttccaaggg
     5941 ggatttgatg gttagttgcg cggttaataa atacccttgg caaaaattat ttaattatcc
     6001 ttctacaacc aaaatcttac aaatcaatcc caaaaacaat ataaacagga aagacgtgat
     6061 cattgtaggc gctttccgca aggagataaa atgaaacccc aaaaacgaaa aactaaattt
     6121 atcttagtat tacaccaagg taatttgccc aattcacatt gtattttatc tgaattaaac
     6181 ggtaatatac ctttctttaa cggtattttg ccacaaacca aagaaattaa cttattaaaa
     6241 aaagcgaggt gcaaatgaaa cgcaaatttc gcacaacact atttctcata gtgttattcg
     6301 ttcttaccgc cacttct
//
LOCUS       DAOOZV010000082         6206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_11096_length_6206_cov_62.959031, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000082 DAOOZV010000000
VERSION     DAOOZV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11096_length_6206_cov_62.959031"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..804
                     /locus_tag="PLW08_07080"
     CDS             151..804
                     /locus_tag="PLW08_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52400.1"
                     /translation="MKRAYLIIPFAIILLLLTSCATTGEEQATKLVTKYDIALTKVSR
                     GGDETTRSKAPVADTTLVDKLRYHYDDEFMHTIWYASENGFELTLYNASDKNIILDWE
                     KGLYLDYDNIGHRLLISSTPDSEKDKPQSPSTIPPRSNITEIIYSADHTNPSTLLGVY
                     VRTPLLPTEYKKAVRFNGKEMKIQLPVRIDNTTYNYEFTFKIVGVRQEQAKSNLLFG"
     gene            complement(1230..1667)
                     /locus_tag="PLW08_07085"
     CDS             complement(1230..1667)
                     /locus_tag="PLW08_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL52401.1"
                     /translation="MKIVPYRKTNELRPVSNMLSLFDEFFNRFYEEEGEEDNFRAMAI
                     DIVEHDKDFEILANLPGFKKDDVKISIHDNQLMIEANSNVTKEETKGTVYRCERYSGS
                     YRRNLLLPENVEVSKISAKMEDGVLKVIIPKKEPSPKKEITIE"
     gene            2208..4007
                     /gene="lepA"
                     /locus_tag="PLW08_07090"
     CDS             2208..4007
                     /gene="lepA"
                     /locus_tag="PLW08_07090"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPL52402.1"
                     /translation="MKEEFIRNFCIVAHIDHGKSTLADRFLEETHIIGKGTEVSQILD
                     SMDLEREKGITIKSHAVRMVHNYQGQDYVLNLIDTPGHVDFSYEVSRALASCEGAILL
                     VDASQGIEAQTMSNLYLALDNNLEILPVINKIDLPKADIEGTENDLCEILGCLPEEII
                     KVSAKTGEGVKDLLDAIVTRLPAPKGNPDTAPRALIFDSYFDMYRGVVVLVRVFEGKL
                     QKGDKIKLFSTAREYEIEEIGYLGLKFSPQTELTCGEAGYIIANIKEVADARVGDTIT
                     LSKGGCENPLPGFREPKPMVYSSIFPINGEDYENLVEAIAKLKLNDASLIYEKESSAA
                     LGYGFRCGFLGMLHLEIVKERLLREYNIPIMATTPSVRFLIKLKSGEELQINNPVDFP
                     DPSTIESIMEPFMDTEIIVPTDYIGNVLKLVQERRGIQKDIQYIDEKRVTLHYELPLI
                     EIIFDFYDKLKTVSRGYASLDYTFKDYRETDVVKVDILINGEKVDAMSFICHQDKAYN
                     WGKSVTETLAEVIPKHLFKIALQAAIGGKIIARSTINPLRKDVLAKCYGGDVSRKRKL
                     LEKQKEGKKKMKEIGSVSVPQEAFLAVLKADRE"
     gene            4011..5690
                     /locus_tag="PLW08_07095"
     CDS             4011..5690
                     /locus_tag="PLW08_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPL52403.1"
                     /translation="MVFFQSRHCFLTLFFCLSFGLILAINIGNISFQGISGIEEIELF
                     KASGLQKGQEYNPELISNATSNLYKYLQNKGRYFVWISSPELIPEDEGTVSLLFKMQE
                     KAPSDEVKIRFQGMQYFSEAKLFQLLLLSPERNYHLNQLSRIMQQTLSLYQSRGYLFT
                     KVELDSLVWEEALSAYIGIDEGKPLRVKNYIFKGNKVTKDKTLLHLSGLENIDLITPE
                     VLTQAEENIQRKSYIRNCNVEPVNENTLLINIEEGKMTYLEGVLGMNRINEELKLSGQ
                     IRLQFLNLWGTDRAIKLFWKQIPTSNSELSLAYHESGIPGIPIAGDIELYRAQQDSTW
                     IKTRGSLEIYYQMLKHSLGIELATESLNPGARRPAIINKEKAQSIGAFWNYSNYAGGV
                     NPYKGMEMKFLYRLTGSNLTKHLYGATEAGVKGYIPIKKNRFVGFIGTEIRNLENKNA
                     ELWQQYKMGGYGTLRGYYEDEFSSFRLAWINYELRYRLNPDSRIYLLFDQGFLGKEKN
                     KLKTDLFGLGFGMKINTRLGILGLEYALGYRDKHFANLGSGMIHLGLDVAF"
     gene            5907..6098
                     /locus_tag="PLW08_07100"
     CDS             5907..6098
                     /locus_tag="PLW08_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52404.1"
                     /translation="MKKVLLITLLAMLLISTLVTGCKKKAEEPQEEVAPIEETTPAPD
                     TTVAPTQSQTPTTTTPTGK"
BASE COUNT         1985 a         1143 c         1241 g         1837 t
ORIGIN      
        1 gatgccctca aagaacctga gaaacaacga gttggaattt acaccaacat ttggggctaa
       61 acttaatttg aggtattgct gaacagtatg gacagaatgc ctacatagat gaacagaagt
      121 aaactaaaag cgctaaaaaa aggagttaaa atgaaaagag cttatttgat tatacccttt
      181 gccattattt tattgctgct tacctcttgt gctacaacag gcgaagagca ggcaaccaaa
      241 cttgttacca aatatgacat tgcccttacg aaagttagta gaggcggaga tgaaactaca
      301 cgaagtaaag ctcctgtggc tgatactacc ctagtagata aattgcgtta ccattatgat
      361 gacgaattta tgcacactat ctggtatgct tccgaaaatg gctttgaact gaccttatac
      421 aatgcttcgg ataagaatat tattctggac tgggaaaagg gtttatatct ggattatgac
      481 aatataggcc atcgcttgtt gatttccagc acccctgatt cggaaaaaga taaaccccaa
      541 tcacccagca caatacctcc ccgttctaat attacggaaa ttatctattc tgccgatcat
      601 accaatcctt caaccctttt aggtgtttat gtgcgcactc ctcttctgcc aacggaatac
      661 aaaaaagcag tccgattcaa cggtaaggaa atgaaaatcc agcttcccgt gagaattgat
      721 aataccacct ataactatga atttactttc aagattgttg gagtgcgtca ggaacaggca
      781 aaaagcaatc tgctattcgg ataattagtt aagcttccct attattaaag gttggcaata
      841 aagacaaggt ggacttgtag agtccttttt ggcaagtata atttgatttt ccaagaagca
      901 ttgcttaccc attgcttacc caatactaat gccatccgta atgggtaagc aatgagtaag
      961 taatgctccc aagtaagaaa ctgtattttc ttctcttatt gtaagataac aaacaaaata
     1021 aaatatttcg ggagccatct ctgccaaaat aacttctaca ctacctgcaa attctttggg
     1081 cagtgagaac tcataacgct ttattgggta aacggaggat gccattcctt agccaaaaat
     1141 tatgaaaagc tttagctacc tccgactaca caaaaaaaac aggattgcgc ttttcagcgc
     1201 aaccctgtaa tttgttacac cataatagat tattcaatgg tgatttcttt tttgggagag
     1261 ggctctttct tcggaattat aactttcaaa accccatctt ccatcttggc tgagattttg
     1321 gaaacctcaa cattctcagg aagtaagagg ttacggcgat agcttccact gtatctttca
     1381 cagcggtaaa ctgtgccttt ggtttcttct ttagtaacat tactattagc ttcaatcatt
     1441 aattgattat catgaatgct gattttaaca tcgtctttct taaatccggg cagatttgcc
     1501 agaatctcaa agtccttatc atgctccaca atatcaattg ccatagcccg gaagttatct
     1561 tcctctcctt cttcttcgta aaaacgattg aagaactcgt caaataagct gagcatattg
     1621 ctcaccggtc tcagttcgtt tgttttgcga tatggtacga ttttcatttt gaacctcctt
     1681 gttcatttta tcgttcacta attattataa tccagttttt ccaaaaagca agaaaaaaat
     1741 tagcagtcaa agtgcaagag tgctaatttc cttttataca aagaaagttt agccaccgag
     1801 aacaccgaaa acaccgagaa caccgatatt ttttatacag agaaaaaaga ttggatttaa
     1861 tatttgtgtt agagcaaaaa tacctttgtc tttttctctc tgccctctgc cctccgctct
     1921 ctgctctaga tttaatacct gtgttaaatt aaatatagct ttgtccttcc tgcgttcttg
     1981 tccctccggt acaagcagga atccacaggc ggcaaaaaat atttatccct tttcaagctg
     2041 aaaccgatat ttgttcttaa gagaacaaag cgaaagtagc tactctgttt atataaccca
     2101 gctgtctaaa aaggataatt gacaaaaagg caaggcaggt atttttggac ttcagttttc
     2161 cgcttcccgg aaatgcagtt taacttgata caaattaagg aaatagaatg aaagaagagt
     2221 ttatccgcaa tttctgcatt gtggcacata ttgatcacgg aaaatctacc ctggcagata
     2281 gatttttgga agagacacat attatcggta aaggaacaga ggtctctcag attttggaca
     2341 gtatggattt ggaacgcgaa aaaggcatca caattaaaag tcatgctgtc cgaatggttc
     2401 ataattatca aggtcaggat tatgttttga acctgataga tactcccggt catgtggatt
     2461 tttcttatga ggtttcccgt gctttggcat cctgtgaagg agccattctt ttagtagatg
     2521 cctctcaagg aattgaggca caaacaatga gcaatctgta tcttgcatta gataacaacc
     2581 tggagatttt acctgtaatt aataaaattg acttacccaa ggcagatatt gaaggcacgg
     2641 aaaacgattt atgcgaaatt ttaggttgcc tacccgaaga aataattaaa gttagtgcta
     2701 aaacaggcga aggagtaaaa gaccttttag atgctatcgt aactcgttta cctgctccta
     2761 aaggcaatcc cgacacggct cccagagcat tgatttttga ttcctatttt gatatgtatc
     2821 ggggtgttgt tgttttagtg cgtgtttttg aggggaaatt acaaaaaggc gataaaataa
     2881 agctcttttc tacggcaaga gaatatgaaa ttgaagaaat cggctatctg ggcttaaaat
     2941 tttctccgca gacagaactt acctgtggtg aagccggtta tataattgct aatatcaaag
     3001 aagttgccga tgccagagtg ggtgatacaa taactttaag caaagggggt tgtgaaaatc
     3061 ctcttcctgg tttcagagaa cccaaaccaa tggtctatag cagtattttt cctatcaatg
     3121 gagaggatta tgaaaatttg gtggaagcca ttgccaagct gaaattaaac gatgcctccc
     3181 taatttatga aaaagaaagt tctgccgctt tgggatatgg attccgttgc ggttttttgg
     3241 gtatgctgca tttggaaatt gtgaaagagc gtctgttgcg tgaatataat attcccatta
     3301 tggctacaac tcccagtgtg cgatttttaa taaaactgaa aagcggagaa gaactgcaaa
     3361 tcaataatcc ggttgatttt cctgatccca gcactattga aagtattatg gaacccttta
     3421 tggatacgga aattattgtg cctacggatt acattggtaa tgtgttaaag ttagttcagg
     3481 aacgcagagg aattcaaaaa gacattcagt atatagatga aaaaagagtt actctgcatt
     3541 atgagctacc tttaattgaa attatctttg atttttacga taaactgaaa accgttagta
     3601 ggggttatgc ttctttggat tatactttta aggactatcg ggaaacagat gtagtgaaag
     3661 tggatatctt aataaacggt gaaaaggttg atgcaatgag ctttatctgc catcaggata
     3721 aggcgtataa ttggggaaaa agtgtaactg aaaccctggc ggaggttatt ccgaaacatc
     3781 tttttaagat tgctctgcag gcagctattg gaggaaaaat tattgcccgc agcactatca
     3841 atcctttgcg gaaagatgtt ttagcaaaat gctacggagg tgatgtttcc cgcaaaagaa
     3901 agctgctgga aaaacaaaaa gagggcaaaa aaaagatgaa ggaaattggt tcggtttccg
     3961 ttccccaaga ggcattcctg gctgtgttaa aagctgatag ggaataaagg gtggtttttt
     4021 ttcagagcag gcattgtttt ttaacactct ttttctgcct ttcttttggg cttattttag
     4081 ctataaatat tggaaacata agctttcaag gcatttccgg aatagaagaa atagagcttt
     4141 ttaaggcaag cggtctgcaa aaaggacagg aatacaatcc ggaacttatt tccaatgcca
     4201 cttctaactt gtataaatat ttgcagaata aaggcagata ctttgtttgg atttcctctc
     4261 cggaattgat tccggaagat gaaggaactg tttccctgct ctttaagatg caagaaaaag
     4321 caccttccga tgaagtaaaa atccgctttc agggtatgca gtatttcagt gaggcaaaac
     4381 tctttcaact tctactgctt tcaccggaaa gaaattatca cttaaaccag ctttcccgaa
     4441 ttatgcaaca aaccctttcg ctttatcaaa gcaggggtta tctctttacc aaagttgaat
     4501 tggattcttt ggtttgggaa gaggcattga gcgcttatat cggaattgat gaaggcaaac
     4561 cgttacgcgt gaaaaattat atctttaaag gaaataaagt tacaaaggac aaaaccctcc
     4621 tgcatctttc cggtttggaa aatattgact tgattactcc cgaggtctta actcaagcag
     4681 aagaaaatat ccagcgaaag agttatatcc gcaattgtaa tgtggaaccc gtaaatgaaa
     4741 ataccttgct gataaacatt gaagaaggta aaatgactta tctggaaggc gttttaggaa
     4801 tgaatcggat taacgaggaa ctgaaattat ctggtcaaat ccgtttgcaa ttcttgaacc
     4861 tgtggggtac cgaccgtgcc ataaaacttt tctggaaaca aattcctact tccaatagcg
     4921 aactttcttt ggcgtatcac gaatcaggta ttccgggaat tcccattgca ggagatattg
     4981 aactctatag agctcaacag gattctacct ggataaaaac tcgcggtagc ttagaaattt
     5041 attatcaaat gctgaaacac agcttaggta ttgaactggc aacggaatcc ttaaatcccg
     5101 gtgccagaag acctgctatt ataaataagg aaaaggcaca aagtataggc gctttttgga
     5161 attatagcaa ttacgcaggt ggtgtaaatc cctataaagg tatggaaatg aaatttcttt
     5221 accgtttaac cggaagcaat ctgacgaaac atttatatgg agctacagaa gcaggcgtga
     5281 agggctatat tcctattaaa aaaaatcgct ttgtgggttt cataggtacg gaaattcgta
     5341 atctggaaaa caaaaatgca gaactctggc aacaatataa aatggggggc tatggaactt
     5401 tgcgtggcta ttatgaagat gaattcagct cttttcgcct tgcctggata aattatgaat
     5461 tacgctatcg ccttaatcct gattcgcgta tttatctact ctttgatcag gggtttttag
     5521 gcaaggaaaa aaacaagtta aagaccgatc tttttggtct cggcttcgga atgaagataa
     5581 atacacgctt aggaatttta ggattggaat atgctcttgg ttatagggat aaacactttg
     5641 ccaatcttgg ttccgggatg attcatctgg gcttggatgt ggcgttctaa aaaaaatact
     5701 tgccaagatt ttggtatcct aaaatgatgt cttcaataac taagtatata gatgtgacta
     5761 aggactgtgt gaaggataga tgagagtttt ttaactctat caggccttca atagaaccgg
     5821 caaattttta tatgtcttgc caaaacaaat caaaaaaaag aaaaaaacct aagtacccga
     5881 aagggaattt gcttatggag gaatcaatga agaaagttct cttaatcacc ttgctcgcta
     5941 tgttgctgat cagcacattg gttactggct gcaaaaagaa agcagaagag ccccaggaag
     6001 aagtagctcc tattgaagaa actactcctg ctcctgatac aacagtagct cctacacaga
     6061 gccaaacacc tacaactact acaccaactg gtaagtagtt tatcagctaa taaacaatag
     6121 cgcagtcctt aaaaaaacgg gtgtcttcgg atgcccgttt tttattttaa gctgattttt
     6181 agccaccgaa aacaccgaaa gattta
//
LOCUS       DAOOZV010000083         6136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_11258_length_6136_cov_56.352574, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000083 DAOOZV010000000
VERSION     DAOOZV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_6136_cov_56.352574"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1014)
                     /locus_tag="PLW08_07105"
     CDS             complement(169..1014)
                     /locus_tag="PLW08_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPL52405.1"
                     /translation="MFITVPVPKNEQLNSYAPGSCEREKLKKALSELHNDFIFAHTSA
                     DVKSLAVACLRGAFEFQGQKCSAASRLYIPDTIYDEWFKEMVEMLETVKMGDVMDFSN
                     FINAVIDKESFDRIKNYIDYAKNSPVAEIIWGGNCDDSVGYFIQPTLIKTTDPMFCTM
                     QEEIFGPVLTVYVYPEKDFSKTLDLCDKTSPYGLTGSIFAQDRKAILEANNVLRHSAG
                     NFYINDKPTGAVVGQQPFGGGRSSGTNDKAGSALNLQRWISARSIKENFVPDIEYKYP
                     FMAEA"
     regulatory      complement(1094..1212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(1367..2464)
                     /locus_tag="PLW08_07110"
     CDS             complement(1367..2464)
                     /locus_tag="PLW08_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52406.1"
                     /translation="MKKILTFISTLLLISIISAINFDYDADMLTRAAAYNDYYKKDGG
                     HIDSRLRLGLNSELTEGLNFRLAIEIGEITWGDNATGGNIGTGGINIETSELYLDYYM
                     PAIKSNIKAGLQYLADHRSLVLDHYGAGVILYTDIEGMKAQAGMLKNLENNRFAHDDY
                     TIFLASLEATSPVVWGFNSYVSYNDRNDDSEISFLPYVTLTAGPATLDLNPFVCYQFI
                     DGPDKTGFGAAVKAEIDVTPIQVGADILIASEDGLVPYCSYYQNGLFIYGYNIIHDGL
                     NLYWTDELYHNNEETFISAVGSIRFPVTEKHTLFAAGGWLKDLGMELNGGIESQLVKD
                     MLKLYLYGAWGKNDDSDVNNYATGVSLQLNF"
     gene            complement(3024..4082)
                     /gene="arsB"
                     /locus_tag="PLW08_07115"
     CDS             complement(3024..4082)
                     /gene="arsB"
                     /locus_tag="PLW08_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPL52407.1"
                     /translation="MQQTTEISKGISFFEKYLTFWVIICMAVGVLIGKYIPEIPYFLR
                     KFEFAQVSIPIAVLIWLMIYPMMMKVDFASIKNVGKNPQGLIVTWIVNWLIKPFTMYA
                     IAWFFFYIVFKSVIPTNLAKDYLAGAILLGAAPCTAMVFVWSHLTNGKPAYTVVQVAT
                     NDLIILVAFTPIVALLMGISGIKIPWDTLILSVILFVVIPLAGGATTRYSVIKHRGED
                     YFLKTFIPKFNNVTISGLLLTLVIIFAFQGEVIINNPLHIILIAIPLTIQTYLIFFIS
                     YIFTKQIKLPHNIAAPAALVGASNFFELAVAVAISLFGADSPVALATTVGVLVEVPVM
                     LNLVSIANKTKQWFIPAN"
     gene            complement(4189..5280)
                     /locus_tag="PLW08_07120"
     CDS             complement(4189..5280)
                     /locus_tag="PLW08_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPL52408.1"
                     /translation="MKYLPVDVLQSFMQEVFIKLGVPEEDAQICSDVLIASDLKGIES
                     HGIGRLKMYYDRIKAGIQNPVTNIDVLRDKCATAVWDGNNGMGQVISKKAMQAAIDKA
                     EKYGLGAVAVRNSTHFGICGYYAEMAIRQNMIGLVFSNARPSICPTNGVSPILGTNPI
                     CFGAPTDLPYPFLYDAATSISQRGKVEQLAREEKETPLGWAIDLEGKPYTDTKGLLID
                     LLKQKAAMLPLGGTEEITGSHKGYGLATAVEILCASLQKGNYLNGLMGEDENGKPAPY
                     RLGHFFMAINIEFFTDLVDFKKITTSICKDLQNSQLFPGKNRIYVAGEKEYEIEQKVR
                     KQGIPIIPNLEKNIRIIQDELGLNLLNLD"
     gene            complement(5294..>6136)
                     /locus_tag="PLW08_07125"
     CDS             complement(5294..>6136)
                     /locus_tag="PLW08_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPL52409.1"
                     /translation="KYFYEKGVRLFATDSKLDLANLAKYAPGSKVLFRILTEGTGADW
                     PLSRKFGSHPDTIYHLIVKAAKMNLQPWGISFHVGSQQRDIGQWDDAVARCKYLFDAV
                     AEEGIELKMINIGGGFPANYVDPTFSIQEYATEIQRFITEDFGDNLPEIILEPGRSLV
                     ADAGVIVSEVINVARKSKNNLYQWVYLDVGKFGGLIETIDESIKFPIYFPRKGLAEEI
                     ILAGPTCDSMDILYEHYRYRMPEGTVPGDRVYIFTTGAYTQSYSSVNFNGFPPLKAVI
                     FEEK"
BASE COUNT         1982 a         1227 c         1041 g         1886 t
ORIGIN      
        1 tcacctcatt tttcctcaac tgcattgttc aggtctatgc attatccgta taaaattgtt
       61 taatggattg attatcctga ataatcagga ttccttttta ttaaaaaaat aaagcagggt
      121 ggcaattgag caattgccac tctgcacatt agtaagtgat aattttaatt atgcctcagc
      181 cataaaaggg tatttgtatt caatatccgg aacaaagttt tctttaatag acctggctga
      241 aatccatctt tgtaaattta acgctgaacc ggctttatca tttgtaccgg aagaccttcc
      301 acctccaaaa ggttgctgtc caactacagc gcctgttggt ttatcgttaa tgtaaaaatt
      361 tccggcacta tgccgtagaa cattgtttgc ttccagaata gcttttctat cctgagcaaa
      421 aatagaaccc gttaaaccat aaggagaggt cttatcacaa agatcaagag ttttgctgaa
      481 atccttttcc ggatatacat aaacagttaa aaccggacca aagatttcct cttgcatagt
      541 gcaaaacatc gggtctgtgg ttttaatgag agtgggttga atgaagtagc caacggaatc
      601 gtcacaattt ccaccccata taatttctgc cacaggtgaa tttttggcgt aatcaatgta
      661 gttcttgatt ctatcaaaac tttctttatc tataacagca ttgatgaagt tggaaaaatc
      721 cataacatca cccattttaa ctgtttccag catttccacc atttctttga accattcatc
      781 gtaaatagta tccggtatat ataaacggga tgcggcactg catttttgac cctgaaattc
      841 aaaagcacct cttaaacagg caactgctaa ggacttaaca tctgccgaag tatgagcaaa
      901 aataaaatcg ttgtgcagtt cactaagtgc tttttttagc ttttctcttt cacaagagcc
      961 gggagcataa ctattcagtt gctcattttt aggaacggga acggtaataa acatttctat
     1021 ctccttttag tttatttcgt tattttcaga atgaaaagga gaaatagaca tagatggacc
     1081 aggcacacct atcctgtggc tcaattcttc accttttcac aatggaaggc aaatccgttt
     1141 tcagataaac cctatcggct aacatccctg ccggagaagt ttaggactgt tagctcggtg
     1201 acaaaacaac cttttaatta tgcaatatta tatcaagtta tgtaaaatag tttactctat
     1261 aaatcgctat agttgatata cataactggt tgaaattatt gttaatgaaa aaagcccggc
     1321 tgtgcaaaac cgggcttcta ataggggatt tttttaagta gtttaattaa aaattaagct
     1381 gcaaggaaac tccggtagca tagttattca catcactatc gtcatttttg ccccaggcac
     1441 cgtaaagata aagtttcaac atatctttaa ccagctggga ttctatgcct ccattcagtt
     1501 ccattccaag gtcttttagc cagccacctg cagcaaataa agtatgtttt tccgttaccg
     1561 gaaaacgaat agagccaaca gcactgataa atgtctcctc attattgtga tataactcgt
     1621 cagtccaata aagattcagt ccatcgtgaa tgatattata gccataaatg aagagtccgt
     1681 tttggtaata tgaacagtaa ggaaccaaac catcctcgga ggcaatgaga atatcagctc
     1741 caacttgaat aggagttaca tcaatttctg cttttacagc agcgccaaaa cctgttttat
     1801 caggaccatc aataaactga taacatacaa acggattcaa atctaaagta gcaggacctg
     1861 cagtaagagt tacataaggc aggaaagata tttcactgtc atcatttcta tcattatagc
     1921 taacataact attaaatccc caaaccacag gagaagttgc ttccagggat gcaaggaaga
     1981 ttgtataatc atcgtgagca aaacggttat tttccaggtt tttcagcatt cctgcttgag
     2041 ctttcattcc ctcaatatcc gtataaagaa taactcccgc accataatgg tctaaaacca
     2101 aactacgatg atccgctaaa tattgtaaac cggctttgat gttgctcttt atggctggca
     2161 tataataatc cagataaagt tcactggttt caatatttat gccacctgtt ccgatgttac
     2221 ctccggtagc attatcaccc caggtaattt ctccaatttc aatagccaac ctgaaattta
     2281 acccttccgt caattctgaa ttgagaccca acctcaaacg actgtctata tgaccaccat
     2341 cctttttgta ataatcatta taggcagcag cacgcgtaag catatcggca tcataatcaa
     2401 aattaatggc acttataatg cttattaaca atagggttga gatgaaggta agaatttttt
     2461 tcacttttct ctcctttttt tatttgtctt taataataag ataattcatt aacctgccaa
     2521 tgtcaagtga atagcaaaag aaacgcattc gtctaatccg aaaataaaag cattgagttg
     2581 gcattataag agcattaaaa attatttcct gctggaatat tgtagcagtt caaatcaata
     2641 taaacacatc caagaagctt taaaaatttc tcgttccatt gtagtaataa tagcatatac
     2701 ttaaatttcg gatgtctatt gccgagctat ttatgttgca agttgtaaag gcaattcgaa
     2761 tcatctaact atatctaaat actcccgtcg gactaactat cactcagttg aatgcttcca
     2821 ctcttcagcc atcctggaat gctgttgcgc aagcaaattc atacaaagtt ttggctgctg
     2881 aggatcctaa ttctgcagaa cggacaataa ttctggaaaa tatagctgca acccagctaa
     2941 atattcaaat tttaccggaa cagccatttc gtttctacaa agtggttgca gttagataat
     3001 aatatcggaa gataatctaa actctaattt gcagggataa accactgctt ggttttatta
     3061 gctatactta ccagattaag cataacggga acttcaacta aaacacctac agttgtagcc
     3121 aaggcaacag gtgaatccgc tccgaaaaga gatatagcta ctgccactgc aagttcaaag
     3181 aaattggatg ctccaactaa agctgctggt gcagcaatat tatggggtaa ttttatttgt
     3241 ttggtgaata tatagcttat aaagaagatt aaataggtct gaatcgtcag tggaatggct
     3301 atgaggataa tatgaagagg attattgata ataacttctc cctgaaaagc aaagataata
     3361 acaagggtaa gcaacaaacc gctaatagta acattattga atttgggaat aaaggttttc
     3421 aggaaataat cctctcccct atgttttatc actgaatatc gcgttgttgc tcctccagct
     3481 aaaggaataa caacgaataa gataacggag agaattaatg tatcccaagg gatttttatt
     3541 ccactaatgc ccataagtaa tgctacaata ggtgtgaagg cgaccagaat tatcaaatcg
     3601 tttgtagcta cctgaactac cgtataagca ggtttaccat tagttaaatg gctccagaca
     3661 aaaaccatcg ctgtgcaagg tgcagcaccc aacaaaatgg ctccagcaag ataatctttt
     3721 gctaaatttg taggtataac agatttaaat acaatataaa agaaaaacca ggcaatagca
     3781 tacattgtga agggtttaat taaccaattt acaatccagg taacaattaa tccctgagga
     3841 tttttaccta catttttaat gctggcaaaa tcaaccttca tcatcatcgg atagatcatt
     3901 aaccaaataa gaacggcaat cggaattgaa acctgagcaa attcaaattt tcgcagaaaa
     3961 taaggtattt caggaatata ttttcctatt agaacaccaa ctgccataca tataatcacc
     4021 caaaaggtta gatacttttc aaaaaaactg attcctttac ttatttctgt tgtctgttgc
     4081 atcttatcca cctttttgtt gttatatttt ttaaggggag attttaaatt cattaataat
     4141 tctttaaaga atattagtat tgtgcataga atcctggcaa aatgtgtatc aatctaaatt
     4201 cagtaaattt aagcccagtt catcctgtat tattctaata ttcttttcca ggttaggaat
     4261 aataggaatg ccttgtttac gcactttttg ttctatttcg tattcttttt cgccagctac
     4321 atagattctg ttttttccag ggaaaagttg tgaattttgt aaatccttgc aaatagaagt
     4381 tgttattttt ttgaagtcca ccaaatccgt gaaaaattca atattaatag ccataaagaa
     4441 gtgtcccaaa cggtagggag ctggctttcc gttttcatct tctcccatta aaccgttcaa
     4501 gtaatttcct ttttgtaagg aagcgcagag aatttctaca gcagttgcca gtccataacc
     4561 tttatgactt ccggtaattt cttctgttcc acctaagggt aacattgcag ctttttgttt
     4621 tagcaaatca atcagcagac cttttgtatc cgtatagggt tttccttcca aatcaatagc
     4681 ccaaccgaga ggagtttctt tttcctcgcg agccagttgt tctaccttac ctctttggga
     4741 aatagaagtt gcagcatcgt ataaaaaagg atagggtaaa tcggtaggcg ctccaaaaca
     4801 tatagggtta gtccctaaaa taggagaaac accatttgta gggcaaatgg aaggtcgggc
     4861 attagaaaaa acaagtccga tcatattttg ccgaatagcc atttctgcat aataaccgca
     4921 aattccaaaa tgcgtggaat tacgaacggc aacagcaccc aaaccatatt tttcagcttt
     4981 gtcaattgct gcttgcattg cttttttact tattacttgt cccattccat tatttccatc
     5041 ccagacggca gtagcgcatt tatcgcggag gacatcaatg ttagttacgg gattttgaat
     5101 tccggctttt atacgatcat aatacatttt taggcggccg attccgtgag attcaatacc
     5161 ttttaaatca ctggctatta acacatcgct gcatatttgt gcatcttctt ctggcactcc
     5221 aagtttaata aatacctctt gcataaaact ctgcagaaca tcaacaggca aatatttcat
     5281 ttctaaatac tccttatttt tcttcaaaaa taacagcttt caagggagga aaaccattaa
     5341 aattaacgct ggaatagctt tgcgtgtaag caccggtagt aaaaatgtaa actctatctc
     5401 cgggaacggt tccttcgggc atccgataac gataatgctc atagagaata tccatactgt
     5461 cacaagtagg accagctaat ataatctctt ccgccaaacc tttacgcgga aaatagatgg
     5521 ggaattttat ggattcatca atggtctcaa ttaaaccgcc aaattttcct acatctaaat
     5581 acacccattg ataaagatta tttttggatt tacgagctac attaattact tcggatacaa
     5641 tcacaccagc atctgcaacc agagaacgac ccggttccaa aataatttcc ggaaggttat
     5701 ctccaaaatc ctctgtaata aaacgctgaa tttccgtagc atattcctga atagaaaaag
     5761 tgggatcaac atagttggca ggaaaaccac ctccgatatt aatcattttc agttcaattc
     5821 cttcttcggc aacagcatca aaaagatatt tacaacgagc tacagcatcg tcccattgcc
     5881 ctatatctcg ttgttgagag cccacatgaa aagaaattcc ccagggctgt aaattcatct
     5941 ttgccgcttt cacaattaaa tgatatatag tatcaggatg tgaaccaaat tttctggata
     6001 ggggccaatc agcgccagtt ccttccgtta aaatccgaaa taagaccttt gaaccaggag
     6061 catattttgc caggttagca agatcaagct tgctgtcagt agcaaaaaga cggacacctt
     6121 tttcgtaaaa gtattt
//
LOCUS       DAOOZV010000084         5903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_11753_length_5903_cov_44.968878, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000084 DAOOZV010000000
VERSION     DAOOZV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11753_length_5903_cov_44.968878"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..5604)
                     /locus_tag="PLW08_07130"
     CDS             complement(55..5604)
                     /locus_tag="PLW08_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPL52410.1"
                     /translation="MAKRKFVILLFIIAAVLVGAQTIPYPVDSKEYQDYKEGLIGKMP
                     VQPPVYSNVPKGKQIPLVFQRQQREGLLIPLDDSFQLAMEGNDDYYTSVIQLPFAFDF
                     YGTTYTEFYINNNGNVSFGNPYSTYTSYGFPVNGFPMLAPFWADVDTRSGGQVFYKME
                     SNRVTVIWNAVGYFSNHTDKLNTFELVFTNGWDPVVGLGYNVAFSYGDMQWTTGDASG
                     GQNGFGGVPATVGLNKGDGIYYGLIGRFDHEGIDYYGPGGAPSGVSYLDNQLFRFTAI
                     QGNFLPPVFGNLPAETVNIQEGESIQLHINASSPSPGNTVYAEVQSDFTAGFYADIIS
                     GNPCYINLTINGLRNNVGLHTITITAIDNGTPSLSTVASFSVRIIPRLGDFLIVTNHN
                     SPSINIVDVATNEVFGPFLQGQLGYSYLVDVVVSSDGQFALVSNFNNDTVYQIDLSNL
                     LEPTIAASYNLGFPAEDITLSADNRYAIVADGGTEHYLAVLDLQSRTTVQVLDIYPHY
                     AQGVTIGPDGKVLINDYENGYVHQYILNFVTGNLSYSGVSIPVASCLNTVISPGGNFA
                     IACSYNRDTKVLKLNADNTIDVIQNLGLSSQSAVYSSDGQIALIGEYGSSNDILGKYN
                     VQGNGSLVWQQSFNLPFYSDSGYYGVDVLALSTDNSKAYITDFASEANNILVNINLEN
                     GTQQNLTLPDPTGLAIGTMHLTAYFTATITNTQNSTQVQFHQHCSGNPDSYLWNFGDG
                     QTSTEPNPYHIYYTPGLYTVTLTVYKNGESDSYSQTIEAMFNTQIHLTLDGSPYYYSA
                     TLAISEGSSLIIDNGVTVNFGPQAGLEVWGSISANGVTFSGSETEGWKGIVLHSPVSP
                     VAFNNCNIYNAVVGLKIINSSFTITNLLISKTHSFTGEIGLKVQGASNMVLNNVQVIG
                     YIMGIVFENEARVASSPSLNNTRIRNTSSTLRTESHGLDVLGAVGLTVNNAEIEEYDY
                     GVYWNGLGTTDYRSTPSLNNTRIRNTSSTLRTVAKGVKMIDISRVTMTNDSIIGYPTG
                     LEITNDGSGLLTTTASLNNTRIRNTSSTLRTETTGINFSGHILATMESLDIDEYSTGI
                     YYEGNGMLSDRATVSLNNTRIRNTSSTLRSVTKGLIMMNIGSVTITNDSIIGYPTGLE
                     ITNDGSGLLTTTASLNNTRIRNTSSTLRTETKGINFTGHILASMDSLYLEDYATGIYY
                     EGNGMPFNRTTPSLNNTRIRNTSSTLREPLKGVVLKNLAGITLNKNIIYPKVQVGQGN
                     LSGNGIFLQTVQNAQIAHNTIWGLNTGLYATGSSNGTFTHNVIWTNGEQLNTPVILED
                     SNIPVSNSDISYAQGVFPGTGNLNADPLFVAPDEGNFYLKPRSPLANQGIGAMPFEFE
                     VLAGVRTYTMHPGWNMMGVPYLTRETYSTPVSIFADDLSPFYVAPTYTSIVQLNPSAP
                     PDTLGHIDMSYTGSYVIPSLVRPGIGYWVRNPYATPATIDVYGLMDDGSYNVVLDGTE
                     SPNNGWYLLANPYDLPIGLNNGIILGEGMLVGLLKYNYVTNSYYFIDSEHPEVIEPWG
                     GFFVKARAQDAHLYLNYPSQARQENENQPFVDDIPNVVSRPKLKWEFSLNAKAGEKGD
                     AIILGVGEGTNDTLDPLDIPEIPNPPFPQTGILQFSICNNDWENNAGLYTRDIKNSRD
                     GSWSWNLLLNLENMLEDGIFSDIIRISALGAEKIPAGYIFRIVNPADGSSVDLRNEDF
                     LLNVEIFHSEGEPWLIPLCLEVIPLANDSGEREVFSASNYPNPFNPETTISYNLPAES
                     FVTLEIYNLKGQLVKRLVSENKPSGAHKAVWNGADQQGREVASGFYFYRLKTKDNQLT
                     RKILLMK"
     gene            complement(5606..>5903)
                     /locus_tag="PLW08_07135"
     CDS             complement(5606..>5903)
                     /locus_tag="PLW08_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52411.1"
                     /translation="SMLEPVPVTLAINALQNQVRLSWQRRRDEESYYVYWSENPYAEF
                     PEGWNGPVNVTETSFTDTLSDKCFYRVFAVPNMRGEIENKQNTKPRAIMPKRGE"
BASE COUNT         1828 a         1247 c         1086 g         1742 t
ORIGIN      
        1 attctcattt ttaatctgcg tcatctgcgt aatctgcgtg agtccttttt ttcttcattt
       61 cataagtagt attttccttg tcagctgatt atccttcgtt ttcaagcgat agaaatagaa
      121 tccggaagca acttcacgac cctgttgatc agctccgttc caaacagctt tatgtgcacc
      181 ggaaggttta ttttcgctta ctaatctctt gaccaattga cctttcaggt tataaatttc
      241 caaagtaaca aagctttcgg caggaaggtt ataactgatt gtagtttccg gattgaaagg
      301 attgggataa ttgcttgcag aaaatacttc tctttcacca ctatcattgg cgagaggaat
      361 aacttccagg cataaaggaa ttaaccaggg ttctccttct gagtgaaaga tttctacatt
      421 cagcaggaaa tcctcatttc ttaaatcaac agagctacca tcggcaggat ttacaatgcg
      481 gaaaatgtaa cccgcaggaa tcttttctgc acctaaagca ctgatgcgga ttatatcact
      541 aaaaattcca tcttccagca tattttccag gttgagtaat aaattccagc tccaggaacc
      601 atccctgcta ttttttatat ctctggtata aagtccggca ttattttccc aatcattatt
      661 gcaaatactg aattgcagta ttccggtttg cgggaaaggt ggattgggaa tttcaggaat
      721 atctaatgga tcaagagtat catttgttcc ttcgccaacg cccaaaataa tagcatcacc
      781 tttttctccg gctttggcat taagagaaaa ctcccatttc agtttgggac gggaaactac
      841 attggggata tcatctacga agggctgatt ttcattctcc tgacgcgcct gtgaaggata
      901 atttaaatac aaatgagcat cctgagctct ggctttcaca aaaaatccac cccacggctc
      961 aataacttcg ggatgttcgg aatcaataaa ataataacta ttggtcacat aattatactt
     1021 tagcaaacca accaacattc cctctcccaa aatgatacca ttattcagac caataggaag
     1081 atcgtaagga ttggctaaaa gataccagcc gttattagga ctctcggttc catctaaaac
     1141 aacattataa ctgccatcat ccattaaacc gtaaacatct atcgttgcag gggtggcata
     1201 aggattgcgt acccaataac ctattccggg acgcacaaga gaaggaataa catagctacc
     1261 ggtgtaactc atatcaatat gacctaaggt atcaggtgga gcagatggat ttaattgcac
     1321 aatggaagtg taagttggag ccacataaaa aggagaaaga tcatccgcaa atatgcttac
     1381 aggagtgcta taggtttctc ttgtcaaata tgggacaccc atcatattcc atccaggatg
     1441 catagtataa gttcgcactc ctgccaacac ctcaaattca aaaggcatag ctccaatgcc
     1501 ttgattggca agaggagaac gcggtttcaa atagaaatta ccttcatcag gggctacaaa
     1561 tagagggtct gcattcagat tacccgttcc cgggaataca ccctgagcat aagaaatatc
     1621 actattgcta acaggtatat tgctatcttc caggataaca ggagtattta attgttctcc
     1681 attagtccag ataacattat gcgtaaaggt accgttacta ctacccgtag catataaacc
     1741 agtatttaaa ccccaaattg tgttatgagc tatttgcgcg ttttgcactg tttgcagaaa
     1801 aataccatta ccactcagat ttccctgtcc aacctgcact ttcggataaa tgatattttt
     1861 atttaaagtt attcctgcta ggttttttaa tactactccc tttaagggtt ctctcaaagt
     1921 gctgctggta ttgcgaatgc gggtattatt taaagatgga gttgtcctgt taaaaggcat
     1981 tccatttcct tcataatata ttcctgtggc ataatcctca agatatagag aatccattga
     2041 agccaaaata tgaccggtga agtttatacc cttggtttca gttcttaaag tgctgctggt
     2101 attgcgaata cgggtattat tcaaagaagc tgtagtagtt aaaagtccgg aaccgtcatt
     2161 ggtaatttcc aagccggtgg gataaccgat aatggaatcg tttgttatag ttacactacc
     2221 gatattcatc attattaaac ctttagttac ggaacgaagt gtactactcg tatttctaat
     2281 cctggtatta tttagagaaa cagttgcacg atcagaaagc attccattgc cttcatagta
     2341 aattccggta gaatattcat ctatatccag agattccata gtcgctaaaa tatgaccgct
     2401 aaagtttatg cccgtggttt cagttcttaa ggtgctgctg gtattgcgaa tacgggtatt
     2461 attcaaagaa gctgtagtag ttaaaagtcc ggaaccgtca ttggtaattt ccaagccggt
     2521 gggataaccg ataatggaat catttgtcat agtgactctg ctgatatcaa tcattttcac
     2581 acctttggca acagtgcgca aagtactgct cgtatttcta atcctggtat tatttaaaga
     2641 aggagtgctt ctataatcgg ttgttcctaa accattccag taaacaccat aatcgtattc
     2701 ctcaatttct gcattgttta cagttaagcc cacagctccc aaaacatcca gtccatgact
     2761 ttccgttcta agagtactgc tggtattgcg aatgcgggta ttattcagag aaggactgct
     2821 ggcaactctt gcttcatttt caaaaacaat gcccataatg taaccaatca cctgcacatt
     2881 atttaatacc atattagatg cgccttgcac ttttagtcca atttctccgg taaaagaatg
     2941 ggttttacta atcagcaaat tggtaattgt gaaactgctg tttataatct ttaagccaac
     3001 tacagcatta tagatattgc agttattgaa agctacagga gaaacgggag aatgcaaaac
     3061 aattcctttc cagccctctg tttcacttcc ggaaaaagta actccattag cagaaattga
     3121 accccaaacc tctaaacctg cttgcggacc gaagtttacc gtcactccat tatcaattat
     3181 aagagaactc ccttccgaaa tagcaagggt agctgaataa taatagggtg agccatctaa
     3241 ggtaagatga atttgggtat tgaacattgc ctctatggtt tgtgaatagc tgtcactttc
     3301 gccattttta tacacagtta gcgttacagt atataaacct ggagtgtaat atatatgata
     3361 aggattgggt tcagtgctgg tttgtccatc tccaaaattc cacaaatagg aatcaggatt
     3421 tccactgcag tgttgatgaa attgcacctg ggttgagttt tgagtattag ttatggtagc
     3481 tgtaaaatat gctgttaagt gcattgttcc aatagctaaa cccgtaggat caggcagggt
     3541 taaattttgc tgagtgccat tttccagatt gatattaact aaaatgttgt tggcttctga
     3601 ggcaaagtca gtaatatatg ccttactgtt atccgtactt aaagccaaaa catccactcc
     3661 ataatatcca ctatctgaat aaaaggggag attaaaactc tgctgccaaa ccaagctacc
     3721 atttccctgc acattatact tccctaatat atcattggag ctaccatatt ctccaattaa
     3781 ggcaatttgc ccatctgaag aataaacagc cgattgagaa cttaatccca ggttttgtat
     3841 tacatctata gtattatcag cattaagttt taggacttta gtatctctat tataagaaca
     3901 ggcaatggca aaattcccac caggactaat aactgtattc aaacaggacg ctacaggaat
     3961 ggaaacacca gaataggata aattgccggt tacaaaattc agaatatact gatgcacata
     4021 accattttca taatcattta tcagcacctt gccatcagga cctattgtaa caccttgagc
     4081 ataatgagga taaatatcca aaacctgcac tgtggttctg gactgtaaat ccaatactgc
     4141 caaataatgt tcagttcccc catcggctac aatagcataa cggttatcag cactcaaagt
     4201 aatatcctcc gcaggaaaac ccaagttata agaagcagcg attgtcggtt caagtaagtt
     4261 gctaaggtct atttggtaga cggtatcatt attaaaatta gagaccaggg caaattgccc
     4321 atcggaggaa accactacat ctacaagata actataaccc aactgacctt gtaaaaaagg
     4381 accaaagacc tcattagtgg ctacatctac aatattgata ctgggtgaat tatgattggt
     4441 aacgattaaa aaatctccca agcgaggaat aatcctcacc gaaaagctgg ctaccgttga
     4501 tagagaagga gtcccattat caatagccgt aatagttatc gtatgcaaac ctacattatt
     4561 gcgtaaaccg ttaattgtca gattgatata gcaaggattt ccggaaatga tatctgcata
     4621 aaaaccggca gtaaaatcac tttgcacttc agcataaaca gtatttcccg gggaaggcga
     4681 agaagcattg atatgtaatt gaatgctttc tccttcctgt atgttaaccg tctctgcagg
     4741 aaggttacca aaaacaggag gcaggaaatt tccctgaata gcagtaaacc tgaataactg
     4801 gttatccaga tagctaaccc ctgaaggtgc tcctccggga ccataatagt caatgccttc
     4861 atggtcaaat ctgccgatta aaccatagta aattccatcg cctttgttta aaccaaccgt
     4921 tgccggaact cctccaaaac cattttgacc tccggaagca tctccagttg tccattgcat
     4981 atctccataa ctgaaagcaa cattataacc caaccccaca acagggtccc aaccattagt
     5041 aaaaacaagt tcaaaggtat tcagtttatc ggtatgatta gaaaaatagc caacggcatt
     5101 ccagattacc gttactctgt tggattccat tttgtaaaag acctgaccac ctgaccttgt
     5161 atctacatct gcccaaaaag gagccagcat cggaaatccg tttaccggaa atccataaga
     5221 agtgtaggta ctgtaaggat tgccaaagga aacattgcca ttgttgttga tgtaaaattc
     5281 cgtgtaggta gttccgtaaa aatcaaaagc aaacggcaat tgaatcacgg aagtgtaata
     5341 atcatcattt ccttccattg ccagctggaa actatcatcc aacggaatta aaagaccctc
     5401 cctctgttgt ctttgaaaca ccagaggaat ctgtttccct tttggcacat tggaataaac
     5461 cggtggctga acaggcattt tacctatcaa accctctttg tagtcctgat attccttgga
     5521 gtctacagga tagggaattg tttgggcgcc aactaatact gctgcaatta taaacagcag
     5581 aattacaaac tttctttttg ccattttatt ctcctctttt aggcataatt gccctcggct
     5641 ttgtattttg tttattttct atttctcccc gcatattagg cacggcaaat acccggtaga
     5701 aacatttatc ggagagagta tccgtaaagc tggtctcagt tacatttaca ggaccgttcc
     5761 aaccctcagg aaactccgca taaggatttt ccgaccaata tacatagtag ctttcctcat
     5821 cccttctgcg ttgccaggat aaacgcactt gattttgtaa ggcatttatc gctaaagtaa
     5881 caggaaccgg ttctaacata gat
//
LOCUS       DAOOZV010000085         5792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_12024_length_5792_cov_52.301377, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000085 DAOOZV010000000
VERSION     DAOOZV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12024_length_5792_cov_52.301377"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..274
                     /locus_tag="PLW08_07140"
     CDS             71..274
                     /locus_tag="PLW08_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPL52412.1"
                     /translation="MPKMTVDPNYCKGCGLCIIACPRKIIRLSENINAKGYNYAECIE
                     QEKCTACKMCYITCPDVAITIEK"
     gene            283..1362
                     /locus_tag="PLW08_07145"
     CDS             283..1362
                     /locus_tag="PLW08_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPL52413.1"
                     /translation="MSKILMKGNEAIAEAAIRSGCRLYFAYPITPQSELIEYMAKMMP
                     KVNGTFIQAESEVAAINMVYGAAGSGKRVMTSSSSPGISLKMEGLSYIAGAELPCVVV
                     NVQRGGPGLGDIQPAQGDYFQATKGGGHGDYRLIVLAPSSVQEFADMASEAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKPAKTEEELQELANQHLSWCICPNEDGDKKHHHEINS
                     LELDPLVLEQHVLGLCKKYAEIQKNEIRYETYNISDENEVLCVAWGTASRVVKSAINE
                     VLKEGKSVGLIRPIRVWPYPYEAIKQAIGKNVKKVYVFELNSGQMVEDVKIAVEGKVP
                     VDFWGKVGGVVFTPAEIKEKLEECF"
     gene            1378..2127
                     /locus_tag="PLW08_07150"
     CDS             1378..2127
                     /locus_tag="PLW08_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPL52414.1"
                     /translation="MEIIAKRPEALTKVPFHYCPGCMHGVAHRLIAEAIDENGLINQI
                     AGVAPVGCAVFAYNYFNCDMAEAAHGRAPAVATGIKRARPDMHIFTYQGDGDLAAIGT
                     AEIVHSANRGEKISVFFVNNAIYGMTGGQMAPTTLPNMKTTTSPYGRNVDDIGYPIRM
                     CELLATLVAPYYIERVSLLSPADIIKAKKAVNKAIRYNKENRGFTFIEFISTCPTNWG
                     YEPEQARQWAKENMLPFFKLGVYRDKGEGVE"
     gene            2127..2672
                     /locus_tag="PLW08_07155"
     CDS             2127..2672
                     /locus_tag="PLW08_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPL52415.1"
                     /translation="MTTEMICAGFGGQGVLTIGKFIAQAAMQEGKNVSWLPSYGPEMR
                     GGTANVSTVVSTEPIASPIVSFPDVLVALNQPSIDKFAPSIRPGGLLIYNTNMCPNGC
                     KRDDITKIAVPMNDIASEIGSMIVLNMLATGIIIGKTGIIKYETLEKDLTSFMQEKNP
                     DLLAKNLTAIKRGIEIAKQYT"
     gene            2871..3659
                     /gene="pyrE"
                     /locus_tag="PLW08_07160"
     CDS             2871..3659
                     /gene="pyrE"
                     /locus_tag="PLW08_07160"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPL52416.1"
                     /translation="MEEITLITIATYTNPFEAELAKGLLEDSGFHPNLLNERIMSMYA
                     SIAGDMYKIELQVPENEEKDAKELLTGLEDSDYVTRILKDEGALLEGHFQLTSGKHSN
                     QYIEKARILQNPAAAHNLCKRLAKRLAEYEFEAIISPAYGAIALGFETAYLMGKNFVF
                     TQRKEGEMIIRNGFDLSRMHKIAIVEDILTTGGSIQEVISCVQNKGLEVIAIGILIDR
                     SGGKIEFPVPLESLLCLEIPAYERENCDACKQNIPLEIPGSSDK"
     gene            3794..5245
                     /locus_tag="PLW08_07165"
     CDS             3794..5245
                     /locus_tag="PLW08_07165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL52417.1"
                     /translation="MDKTYRISIVTFFSKEVPLVIDACEFSPIRKELELELICPSEDA
                     LKYKTDNLQLTGLNNINKLPVPTPQQICFFASPASFDYYCKQENINSSYLYVHPDMAV
                     LELKGNNPFHTDLAEYGLCPCVLKEDTAEDLYEKKEKLIYFSDLSQFIENKKHNYVLL
                     STNFRFIEALINIDRENWELFHPELNYRNLRVNIGKPHSILLNAQKQLLNMARPLDDI
                     YDLAGIRFCRRNNSSAYSFFAEHYDDYMAHVDYALWVNSILYWFKQYSPLKLSKILEL
                     ACGTANMSIQFVRKGYTVFACDNSVDMLKIAARKTVKPKLYYASLTDPILDKDYQLVI
                     CMFDSINYLTQTKQIAKVLKEVSLALETGGLFIFDISTLENSMDNFAHLCDLHRYSDG
                     LMIHQAYFEYIQLIQKSSLHFFKKNILGYSYQFEQHQQRVYYTYELIEIIRNSPLKLI
                     AIHSTETKTNLYPKHLSTIDDKFSRLFFVLQKV"
     gene            5248..>5792
                     /locus_tag="PLW08_07170"
     CDS             5248..>5792
                     /locus_tag="PLW08_07170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPL52418.1"
                     /translation="MTILYQNDIEFFNTHGLFAGIDEAGRGALAGPVVTAAVVLNYNT
                     LVEDINDSKLLTPKKREELYRQIVESAIDYSIVEISTAYIDRYNILRATLLGFKKAFF
                     QLKSKPRYCLIDGRDIHSELKPYAKAIIKGDQLYASIAAASILAKVHRDKLMLSYDNI
                     YPEYGFASNKGYGTAYHAKMI"
BASE COUNT         1828 a         1029 c         1170 g         1765 t
ORIGIN      
        1 tttgacagaa taaggcaaaa aaatggttta tcacagaaag gaaaaaaata aaaaaagaga
       61 ggtgtttgtt atgcctaaaa tgactgttga ccccaattat tgcaaaggtt gtggcttgtg
      121 tattattgct tgtccgagaa agattattcg tttatcggaa aacattaatg ccaagggcta
      181 caactatgca gagtgtattg agcaggaaaa gtgtactgcc tgcaaaatgt gttatataac
      241 ctgtcctgat gtagcaataa caattgagaa gtgaggttat gaatgtctaa aattttaatg
      301 aaaggaaatg aggcaattgc agaagccgca attcgtagtg gttgtcgctt gtattttgcc
      361 tatcccatca ccccccaaag cgaattaatt gaatatatgg caaaaatgat gcctaaggta
      421 aacggaactt ttattcaagc ggaaagcgaa gttgctgcca taaatatggt ttatggagct
      481 gctggaagcg gaaaaagagt gatgacatct tcttcctctc cgggtatttc gttaaaaatg
      541 gaaggtctat cttatattgc gggagcggaa cttccctgtg tagttgttaa tgttcaacgc
      601 ggaggtcctg gtttaggaga tatccaaccc gcacaaggtg attattttca agcaaccaaa
      661 ggcggaggtc atggcgatta tcgtttaatc gttttagcac cgagttcggt tcaggaattt
      721 gcagatatgg caagcgaagc atttgatctg gcagataaat atcgcaatcc tgttatgatt
      781 cttgccgatg gaatgctggg acaaatgatg gaacccgttg agtttaaacc tgcaaaaacg
      841 gaggaagaac tgcaagagct tgccaatcaa catctttcct ggtgtatctg tcccaatgaa
      901 gatggcgata aaaaacacca tcatgaaata aattctctgg aacttgaccc cctggttttg
      961 gaacagcatg ttttgggttt atgtaaaaaa tatgcggaaa tccaaaagaa tgaaattcgc
     1021 tatgagacct ataatatttc agatgaaaat gaagttttat gcgttgcctg gggaactgcc
     1081 agccgagttg taaagtcagc tatcaatgaa gttctaaaag aaggaaaaag cgttggctta
     1141 attcgtccca ttcgtgtatg gccctaccct tatgaagcaa tcaaacaagc aattggtaaa
     1201 aatgtgaaaa aggtctatgt ttttgaactc aattcaggac aaatggtaga agatgtaaaa
     1261 attgctgtag aaggaaaggt tccggttgat ttttggggaa aagtgggagg agttgttttc
     1321 acccctgctg aaatcaaaga aaaactggaa gaatgttttt aaaggaagga ggaaaaaatg
     1381 gaaattattg caaaacgccc tgaagcactt actaaagtcc ctttccatta ctgccctgga
     1441 tgtatgcatg gagtagctca tcgtttaatt gctgaggcaa ttgatgaaaa tggtttaatc
     1501 aatcaaatag ccggtgtggc acctgtggga tgtgctgttt ttgcctataa ctattttaat
     1561 tgtgatatgg cagaagcagc tcatggacgc gctcctgccg ttgctacagg cataaaaaga
     1621 gcccgtcctg atatgcatat atttacctat caaggagatg gtgatttagc tgctatcgga
     1681 actgcggaaa ttgttcattc tgccaatcgg ggagaaaaga taagcgtttt ctttgttaat
     1741 aacgctatct atgggatgac aggtggacaa atggctccta caactttgcc taatatgaaa
     1801 actactacca gtccttacgg cagaaatgta gatgatatcg gttatcctat tcggatgtgt
     1861 gaacttttgg caaccctggt ggctccctat tatattgaaa gggtctcact attaagcccg
     1921 gctgatatta ttaaagcaaa aaaagctgtt aacaaagcca ttcgctataa caaggaaaat
     1981 cgcggtttta cctttataga atttatcagc acctgcccta ccaattgggg ttatgaacca
     2041 gaacaagcaa gacaatgggc aaaggaaaat atgttgcctt tcttcaaatt aggtgtttat
     2101 cgtgataaag gtgaaggagt ggaataatga ctacagaaat gatttgtgcc ggttttggcg
     2161 gtcaaggtgt tcttactata ggtaaattca ttgcccaggc agctatgcaa gaagggaaaa
     2221 atgtttcctg gctgccttct tacggaccag aaatgagagg tggaactgcc aatgtttcta
     2281 cggtggtttc tacggaacct attgcttctc caattgtcag tttccctgat gtgttggtag
     2341 ctttaaatca gccctctata gataaatttg ctccttctat tcgtcctgga ggacttttaa
     2401 tttataacac taatatgtgt cccaacggtt gtaagcgtga tgatattact aaaatagcgg
     2461 ttccgatgaa tgatatagcg tccgaaattg gcagtatgat tgtgcttaat atgctggcaa
     2521 caggtattat aatcggaaaa acaggaatta tcaaatacga aaccctggaa aaggatttaa
     2581 cttcctttat gcaagaaaaa aatcctgatt tgcttgccaa aaatcttact gctattaaac
     2641 gcggaataga gatcgctaaa caatatactt aaatagttct ttcctaatgc cgggagccga
     2701 ttttttctcc ttacaatttt ttcggttcct ggcactttta gcttgttaaa ttctttcttt
     2761 gctaaagagg tagatgacct ctaaagtata ttttaaatgg tttcctgcct gcgcaggaat
     2821 gataaagata tttggcaaac aaaaatagcg tcccataaat ggaggataaa atggaagaga
     2881 taactttgat tactattgcc acttatacca atccctttga agcagaactg gcaaaaggtc
     2941 ttttagagga ttccggtttt catcccaatc tgctaaatga acggattatg agtatgtatg
     3001 catccatcgc gggagatatg tataaaatag aattgcaggt tcccgaaaat gaagaaaaag
     3061 atgctaaaga actgctaacc ggtcttgagg attcggacta tgtaacccgc atcctgaaag
     3121 atgaaggtgc tctcctggaa ggtcattttc agttaacatc cggaaaacat agcaatcaat
     3181 atattgaaaa agccagaatt ctgcaaaatc ctgctgcggc tcataacctt tgtaaacgcc
     3241 ttgcaaaacg cttagctgaa tatgaatttg aggcaattat tagtcctgcc tatggagcta
     3301 ttgctcttgg ctttgaaaca gcttatctga tgggaaagaa ctttgtgttt actcagcgaa
     3361 aagaaggtga aatgataatc cgcaatggtt ttgatctttc ccgtatgcat aaaatagcaa
     3421 tagtggaaga tattctcaca actggtggca gtattcagga agttatctcc tgtgttcaaa
     3481 ataaaggtct ggaagtaatt gcaattggta ttctaattga tcgtagcgga ggtaaaatag
     3541 aattccccgt tcctttggaa tcactcctat gccttgaaat tcctgcttat gaaagggaaa
     3601 attgtgatgc ctgcaaacaa aatattccct tagaaatacc tggtagcagt gataaatagt
     3661 ttatttttta atgcttgcga tcatattccg gagagtaatc cctttgtcat tcctgcgcaa
     3721 acaggaatcc agataaagat gaacaacata accgcatttc caatatgaaa actatatagc
     3781 aagagcacca tttatggaca aaacatatcg catttcaatc gttacttttt tctctaaaga
     3841 agttccctta gttattgatg cctgtgaatt ttctcctatc aggaaagaat tggagctgga
     3901 actaatttgt ccttctgaag atgctctaaa atataagacg gataatttgc aattaacagg
     3961 gttgaacaat attaataaac ttcctgttcc aacacctcag cagatttgtt tttttgccag
     4021 tcctgcttct tttgattatt attgcaagca ggaaaatata aattcctcct atctttatgt
     4081 ccatcctgat atggcagttt tggagcttaa aggcaataat ccctttcata cagaccttgc
     4141 tgaatatggc ttgtgccctt gtgtgctaaa agaagatact gctgaggact tatatgaaaa
     4201 aaaggaaaaa ctgatctatt ttagtgattt aagtcagttt attgaaaata aaaaacacaa
     4261 ctatgtttta ctgagcacaa atttcaggtt tatagaagca ctgataaata tagataggga
     4321 aaactgggaa ctatttcacc cggaactgaa ttatcgcaat ctccgggtaa atattggtaa
     4381 gccccatagt atacttctta atgctcaaaa acagctatta aatatggcac gtccgttgga
     4441 tgatatttat gacctcgcag gaattcgttt ttgccgaaga aataactctt ctgcctattc
     4501 cttttttgca gaacactacg atgactatat ggcacatgtg gattatgcct tgtgggtgaa
     4561 tagtatactt tattggttta aacaatattc tcccttgaaa ctttctaaga tactggaact
     4621 tgcctgtggc acagcaaata tgtctattca atttgtgcga aagggttata ctgtttttgc
     4681 ctgtgataac tctgttgata tgctcaaaat tgctgccagg aaaacagtaa aacctaaatt
     4741 atactatgca tctttaaccg atccgatttt agataaagat tatcagctgg taatttgtat
     4801 gtttgacagc attaattacc ttactcaaac aaaacagata gccaaagttt tgaaggaggt
     4861 ctctttagct ttagaaaccg gtggcttatt tatttttgat atttctactt tggaaaacag
     4921 tatggataat tttgcccatc tttgcgattt gcaccgttat agtgatggtt taatgattca
     4981 tcaggcatat tttgaatata tccaattaat ccagaaatcc tctttacatt tctttaaaaa
     5041 gaacattttg ggctatagct atcagtttga acagcaccag caaagggtct attatactta
     5101 tgaacttatt gagattatcc gtaattcacc tctgaaatta attgccatcc atagcacaga
     5161 aacaaaaaca aacctctatc ccaaacattt atccactatt gacgataaat tcagtcgtct
     5221 gtttttcgtt ttgcaaaagg tgtgattatg actatccttt atcagaacga tatagagttc
     5281 tttaacaccc acggtctttt tgccggaatt gatgaagctg gtcgcggtgc tttagccggt
     5341 cctgttgtaa ccgctgcagt ggttttaaac tataatacac ttgttgaaga tatcaacgat
     5401 tccaagttgc ttacgcctaa aaaacgcgaa gaactctatc ggcaaattgt agaaagtgct
     5461 attgactata gtattgttga aattagcact gcttatattg atagatacaa tattctgcga
     5521 gcaacacttt tgggctttaa aaaagcattt ttccaattga aaagcaaacc ccgatactgc
     5581 ctaattgatg gcagagatat tcactcggaa ctaaaacctt atgccaaagc aataataaaa
     5641 ggtgatcaac tctatgcctc aattgccgct gcctcaattt tagctaaagt ccatagagat
     5701 aaactaatgc tttcttatga caatatctat cctgaatatg gttttgcctc taataagggc
     5761 tatggaactg cctatcacgc taaaatgatt tg
//
LOCUS       DAOOZV010000086         5754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_12086_length_5754_cov_55.395508, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000086 DAOOZV010000000
VERSION     DAOOZV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_5754_cov_55.395508"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PLW08_07175"
     CDS             complement(<1..1523)
                     /locus_tag="PLW08_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52419.1"
                     /translation="MPYKNNMKIRHYCVKCLIAIAIFAIAATNLFCYNTGDYRTKWGG
                     NFETLELWECYNGIGWIDATQLPSSPFVNTIYISNQTVTMNSSMIIEGGLVIVGTLQL
                     ASGAILTINPSVNCEIGVIETYSGSTLINNGFITANSSSSSLKVHGGILENNGILTSS
                     APNNCNVYINSNGRINFGNRGSITGNCSFTTNYGSIIATANTQGLDGSLNCSGNISFN
                     QIYLIYNGTEPQITGMKTPDLVLGIDFNNPAGITLSKNVKLIYTALVHSGTTLYFDVH
                     IIKEAWYGSGTFSMEDGSTIATANPDGFWSTGNKGSVQVGTRNYNSNGNYIFNGTEHQ
                     QTGDFNPTPNAYTVNDIIFDNPAGVTLTHPITVVSTLELLEGDINYAVLPQGVDGFYS
                     PDVKKTVIHKNGTLMYNFLADSLPFQNNGEYVKRKWYLKGNFNGSKKVTFYWSENEDD
                     NYNWNVHNFPKVYLSNSNEPLHTIWNPAHPREISFIAHSFPNAKEDVYYYIGKERDDT
                     "
     gene            1749..3356
                     /gene="ispH"
                     /locus_tag="PLW08_07180"
     CDS             1749..3356
                     /gene="ispH"
                     /locus_tag="PLW08_07180"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPL52420.1"
                     /translation="MPKRKIIVAIDGPAASGKSTTAKELAKKLGYVYLDTGAMYRACA
                     LQAIREGISPNDISSIVRMLEKIDIQISYTEEENIIWLNGEDVSQQIRTPEISSSASA
                     ISAIPAVRYKMVELQRKLGKNGGVVLEGRDIGTYVFPEAEAKFFMVADLEVRAKRRFK
                     ELAEKGIKTNYTDVLKELKERDEADANRSLAPLKPAEDAILIDTSNLTIEEQVHKLYE
                     ITCRIIEKIDNNNSDKEPGERKIKIRLANYLGYCFGVKRAIQMAKDAILTEKPVYTLG
                     ELIHNPAIVQELEEQGIHIANKAEEVKNSTVIIRSHGITKEEYKILKDNNNEIIDATC
                     PYVKRTHTILKHLVKEDYPVLILGDKQHQEVIGMRSYGNEKTFVIGENDPIPKIKEKR
                     LAIMAQTTQRIEKLQELVYKLLSNFNELRVFNTICLATTQRQKAAEELAQKSDAMFVI
                     GGLKSSNTRALAKLSSVYCPTYHIEDEKQLASIDISPYKSIGITAGASTPEEMIIKVY
                     NVLLQKSGDFKTVTSIEEIPMFKEESC"
     gene            3350..>5754
                     /locus_tag="PLW08_07185"
     CDS             3350..>5754
                     /locus_tag="PLW08_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPL52421.1"
                     /translation="MLIHDQNTVPPESKETENTNPEVETVLSVGEDKMSKFTVEEETK
                     EEETKEEEPSIAIPEEMLSENAETAVSEEAKPEEPAPEPEQEQEKKELTQEEKEHQEM
                     LSMYEESFSSFKKGEIIEGTVVDITDKDVRVDIGFKSEGIIPISEFAYTGIPELNSVI
                     KVYINEIENGEGKLLLSKKKADYMINIERIKQAFADKSSLSGIIRRRVKGGMIVDVLG
                     IEAFLPGSQMSLKPIPNLDQFIGKELYFKVVNIDEEHHNIILSRRQVLEEEAEQKRQE
                     LLRKIQIDAELDGEVKNITQYGAFIDLGGIDGLLHLTDMSWGKLNHPSEMLQIGDKVK
                     VKVINFDPETNRISLGMKQLVPHPWENIEIKYPEGSRVTGKVVNLKPFGAFVELEPGV
                     EGLVHISEMSWTKKISDAHKFLKIGETINAIVLDIDKENKRISLGMKQMEPNPWLTIE
                     DRYPIGTILTRKIKSLTAFGAFVEIEEGIDGLIHISDISWTKRIYHPREVYKKNQEVE
                     AVVLSIDRAQHRVALGVKQLVPDPWETLDQSLPINTEVKGKIGKLIPKGVLVDIPVNN
                     DVVEGFVPVSHLAIPKLEHSEDAFYIGEEIPLKVIELDMENRRLILSVKAFFFSRDPK
                     LQEEYIALHEQYMREKVARQQKKKQEKETREKKFAQKKEEEETTKPEEKEITPEKTEL
                     IKEEIQETIGLKEEKITEPEQTITEEIQSKIEEQEETKETEKEEEIIPEIKETETIEI
                     PITEEPEPVEMPVMEEPEPIEIPVIEEPEPIEIPVIEEPEPITTEEIPVEIETEEEKI
                     ETE"
BASE COUNT         2089 a          930 c         1138 g         1597 t
ORIGIN      
        1 gtatcatctc tttctttacc gatatagtaa tatacatctt ctttggcatt ggggaaacta
       61 tgtgcaataa aggaaatctc tctgggatgg gcaggattcc aaatagtgtg caatggttca
      121 ttactgtttg ataaatatac ttttgggaaa ttatgaacat tccaattgta gttatcatcc
      181 tcattttcac tccagtaaaa ggttactttt ttagaaccat tgaaattccc tttaagatac
      241 catttgcgtt taacatactc accattattc tgaaaaggaa gagaatctgc cagaaaatta
      301 tacatcagag taccattttt gtgaataacg gtttttttta catcgggact ataaaaacca
      361 tctactcctt gaggtaaaac cgcgtaatta atatctcctt caaggagttc cagagtagat
      421 acaactgtta tagggtgagt taaagttaca cctgcgggat tatcaaaaat aatgtcattt
      481 acagtataag catttggcgt tgggttaaaa tctccggttt gctgatgttc ggtgccatta
      541 aaaatgtagt taccattgct gttataattt cttgttccta cctgcacact gcctttatta
      601 ccagtagacc aaaaaccatc tggattagcg gttgcaatag tagaaccatc ttccatagaa
      661 aaagttcctg agccatacca tgcttccttg ataatgtgta catcaaaata tagtgtagtt
      721 ccgctatgaa ccaatgcggt atatataagt tttacatttt tgcttaaggt tatgcctgca
      781 ggattattaa aatctatacc caaaaccaga tccggtgtct tcattccagt tatttgaggt
      841 tcagttccat tataaataag ataaatttgg ttaaagctga tatttccact gcaatttaat
      901 gagccgtcta aaccttgagt attggctgta gcaatgatgc ttccgtaatt agtagtaaaa
      961 gaacaattgc cggttattga accccgattt ccaaagttaa ttcttccatt agagtttata
     1021 tatacattac aattattagg agctgaggag gtgagaatac cgttgttttc cagtattcct
     1081 ccatgtactt ttagggaaga agaactgcta tttgctgtta taaatccatt gttaattaat
     1141 gttgaaccgc tatatgtttc aattactcca atttcgcaat ttactgaagg attaatggtt
     1201 aatatagctc ccgaggctaa ttgcaatgtt cctactataa caagaccccc ttcaataatc
     1261 atagaactgt tcatagtaac tgtctggttt gatatatata ttgtatttac aaaaggggaa
     1321 gagggaagtt gtgttgcatc aatccaacca ataccattat agcattccca taattcaagg
     1381 gtttcaaaat ttccgcccca ttttgttcta taatctcctg tattgtaaca gaataaatta
     1441 gttgcagcta tggcaaaaat tgcgatagca atcaagcact tgacacaata atgtctaatt
     1501 ttcatattgt ttttgtaagg cataatgtaa ccccttgatc tatacagtgt taagatagaa
     1561 attaaataga taatttaaat tcaaacccaa actttttaat ggtggcatag tgtcaatcat
     1621 ttttatgttt tataggagtt aattgtttgt atataaataa ataaggaata aaagtcagat
     1681 attatactgt gaaaaaaggg ttgacggtat tgtaggaaat aaaaaaaagg cgctaaagga
     1741 gaaaagtatt gccaaagcga aagattattg ttgccattga cggtcctgct gcttccggga
     1801 aaagcacaac agccaaagaa ttggctaaaa aattgggata tgtgtatctt gatacaggag
     1861 ctatgtatcg tgcttgtgca ttacaggcaa tacgggaagg aatttctccc aatgatattt
     1921 catccatagt gcgtatgctg gaaaaaatag atatccaaat atcctatact gaagaggaaa
     1981 atattatttg gttaaatggg gaagatgtaa gccaacaaat tcgcactccg gaaatatcaa
     2041 gttctgcttc ggctatttct gcaattcctg cagtccgcta taaaatggtt gaattgcaac
     2101 gcaaacttgg gaaaaatggg ggtgttgttt tggaaggcag agatattgga acttatgttt
     2161 ttcccgaagc tgaagcaaaa ttttttatgg tagctgattt ggaagtgagg gcaaaacgcc
     2221 gatttaaaga gctggcagaa aaagggataa aaaccaatta tacagatgtc ctaaaggaat
     2281 taaaagaacg tgatgaagca gatgccaaca ggtctcttgc acccttaaaa cctgcagaag
     2341 atgctatttt aatagataca agcaacttaa ctattgaaga acaagtgcat aagctttacg
     2401 aaataacctg cagaataata gaaaaaatag ataacaacaa ttcggataaa gaacctgggg
     2461 aaagaaaaat caagatacgt ttggcaaatt atttgggtta ttgttttggt gtaaaacgag
     2521 caattcaaat ggcgaaagat gcaatattaa ctgaaaaacc tgtttatact ttaggagagc
     2581 ttattcataa tcccgctatt gttcaagaac ttgaagagca gggaattcat attgccaata
     2641 aagcggaaga ggtaaaaaac agcacggtta ttattcgttc acatggaatc acgaaagaag
     2701 aatataaaat actaaaagac aataataacg aaatcataga tgctacttgc ccttatgtaa
     2761 aaagaacaca cactatcctg aagcatcttg ttaaggaaga ttatccagta ttaattctgg
     2821 gtgataaaca acatcaggaa gtgataggaa tgcgttctta tggaaatgaa aagacatttg
     2881 taatcggaga aaacgatccc ataccgaaaa ttaaagaaaa gcgtttggct ataatggcac
     2941 aaactaccca aaggatagaa aagcttcagg aattggtata taaattgctc tctaacttta
     3001 atgaactgag agtgtttaat actatctgtt tagcaacaac acaaaggcaa aaagcggcag
     3061 aggaacttgc ccaaaaaagt gatgcaatgt ttgtgattgg cggtctgaaa agttccaata
     3121 cgcgtgcttt agcgaagttg agttcagttt attgtccgac ttatcatata gaagatgaaa
     3181 aacagttagc atctattgat atttcgccat ataaatctat tggtatcact gccggagctt
     3241 ccactccgga agagatgatt ataaaagttt ataatgtatt attacaaaaa agcggggact
     3301 ttaagaccgt aacaagtatt gaagaaatcc ctatgtttaa ggaggaatca tgttaataca
     3361 tgatcaaaac acagtcccac ccgaaagcaa agaaacggaa aataccaatc ctgaagtaga
     3421 aaccgtttta agcgtcggtg aagacaaaat gtcaaaattt acagttgagg aagaaaccaa
     3481 agaagaagaa accaaagaag aagaaccctc aattgccatt cccgaagaaa tgttaagtga
     3541 aaatgccgaa acagctgttt cggaagaggc aaaacctgaa gaacctgctc ctgaacctga
     3601 acaggaacag gaaaaaaagg agcttactca ggaagaaaaa gagcatcaag aaatgctttc
     3661 tatgtatgaa gaatccttct ccagttttaa gaaaggtgaa attatagaag gcacggttgt
     3721 ggatattacc gataaagatg tccgtgtgga tattggtttt aagtccgaag gtataatacc
     3781 aatttccgaa tttgcctata ccggaattcc ggaactcaat tccgtaatta aagtgtatat
     3841 taatgaaatt gagaacggag aaggtaagtt gctactttct aaaaagaaag ctgattatat
     3901 gatcaatatt gaacgcatca aacaggcatt tgccgataaa agttcattaa gcggaataat
     3961 ccgtcggcgc gttaaaggtg gaatgattgt tgatgtttta ggaatagagg catttttgcc
     4021 tggttcgcag atgtccttaa aaccgattcc caatcttgat cagttcatcg gtaaagagct
     4081 atatttcaaa gtggtgaata ttgatgaaga acaccacaat ataattcttt cccggcgtca
     4141 ggttttagaa gaagaagcag aacaaaaaag acaggaattg ctcaggaaaa ttcagataga
     4201 tgcggaactg gatggagaag tgaaaaatat aactcaatac ggtgccttta ttgatttagg
     4261 cggaattgac ggtttattgc atcttacaga tatgagctgg ggtaaactta atcatccatc
     4321 agaaatgctc caaattggcg ataaagtaaa agtgaaagtg ataaattttg atcctgaaac
     4381 gaatagaatt tccttaggga tgaaacaact ggttccccat ccctgggaaa acatagaaat
     4441 taaatatccc gaaggtagta gagtaaccgg caaggttgtg aacttaaaac cctttggtgc
     4501 ttttgttgaa cttgaaccag gcgttgaagg tctggtacat atttcagaaa tgagctggac
     4561 aaaaaagatt agcgatgctc ataaattctt aaaaattggc gaaacaatca atgccatagt
     4621 gctggatatt gataaagaaa acaaacgaat ttccttaggt atgaaacaaa tggaacccaa
     4681 cccctggctt actattgaag accgctatcc tattggaaca attctaactc gtaaaattaa
     4741 gagtttaact gcctttggtg cttttgtgga aattgaagaa ggtattgacg gtttaattca
     4801 tatttctgat atcagctgga ctaaaagaat ttaccatcct cgcgaagtat ataagaaaaa
     4861 tcaggaagtt gaagcagtag ttctttctat agatagagct caacatcgtg ttgcattagg
     4921 tgtaaaacaa ctggttcctg atccctggga aacattagat caaagcttac ctatcaatac
     4981 tgaagtaaag ggaaaaatag gcaaacttat tcctaaaggt gtattagttg atattcctgt
     5041 aaataatgat gttgtagaag gttttgttcc tgtttcgcat ctggcaattc ccaagctgga
     5101 acatagcgaa gatgcttttt atatcggaga agaaattccc ttaaaggtta ttgaactgga
     5161 tatggaaaat cggcgtttaa ttctctctgt taaggcattc ttcttctctc gcgatcctaa
     5221 actgcaggaa gaatatatag cccttcatga acagtatatg cgggaaaaag ttgctcgtca
     5281 acaaaagaaa aaacaggaaa aagaaactcg cgaaaagaaa ttcgctcaga aaaaggaaga
     5341 ggaagaaaca accaaacccg aagaaaaaga aattactccc gaaaagacgg aactaataaa
     5401 agaagaaata caagaaacta tagggttaaa agaggaaaaa ataactgaac cggaacagac
     5461 aataacagaa gaaattcaga gcaaaataga agaacaggaa gaaacaaaag aaacggaaaa
     5521 ggaagaggaa attattcctg aaataaaaga aactgaaacc atagaaatcc caattacgga
     5581 agaacctgaa cctgtagaaa tgcctgttat ggaagaaccg gaacctatag aaatacctgt
     5641 aatagaggaa ccggaaccta tagaaatacc tgtaatagag gaaccggaac caataacaac
     5701 agaagaaata cccgtggaaa ttgaaactga agaagaaaag atagaaacag aaga
//
LOCUS       DAOOZV010000087         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_12525_length_5573_cov_70.020116, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000087 DAOOZV010000000
VERSION     DAOOZV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12525_length_5573_cov_70.020116"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..277)
                     /locus_tag="PLW08_07190"
     CDS             complement(20..277)
                     /locus_tag="PLW08_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52422.1"
                     /translation="MKTGKIIDVKCRCGYTLFRYYKAGKGRLIKCIISRLTADFVGLK
                     GAETFSRPKCPKCGKELGIIKMIHGEPALKLNQGTIETIRL"
     gene            complement(278..2701)
                     /locus_tag="PLW08_07195"
     CDS             complement(278..2701)
                     /locus_tag="PLW08_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPL52423.1"
                     /translation="MKVNIEHLLPFVEKPSRYIDNEINAWRKDFSTFQVHFAFAFPDT
                     YELGISHLGIKILYSLINELDYAMADRVYLPWIDLIDLMREEELELFGWESRRQVKDF
                     DILGITLQSELNYTNVLELIKLSGIDIHSKQRQETDPIILAGGPCAVNPLPLAPFIDV
                     FFIGEAEEGIIQIAEILKNEKKRSERIKQISKLPSCYVPALHNGIDHIHCRKYTAFHT
                     SKSQHSPQLLSWQLATHNRYVSEIMRGCSRGCRFCFAGYFYRPVREREPNAIIENILK
                     EIKASGWDEVGLLSLSSSDYSCIQNLLLQLLKFIETQKTHIALPSLRVDTLDMEIGQA
                     LKSLGREGLTIAPEAGSEHLRQVINKNLSEEQILAGIETALKFGWQKIKLYFMLGLPS
                     ETEDDIEAIIKLIHKINEQGKRRLQINVTLSPFVPKPFTPFQWCAMEDAESLLKKAIK
                     IKSAFAKAKNIKIRYHTIENSILEALLSRGDTKIAEVIELAFNKGARFDGWNECFNFS
                     WWQEAIVECNINLNSYLGSRNPQDKLPWDFISLGIDKNFLLSEWEKAKAGIQTPDCRE
                     LCSSCGICNEEVQTKTAKGSLLETDNLQQNKKIPLYQASQYKYRVYYQKTGLLRFISH
                     LDWMRMLFRRISVLELKTIFTQGFNPHPKVSLCPPLAVGIEGLAEYFDLSFYQPYSAE
                     LILQEFNKTKIPDFTVTAVEPIKTKITPPRTELLSTIFPDSLYLHIRDKIKFFKLSKI
                     FTYTKGTPPKVKNYDLKEIIVSINLIGNELQLEKLLESPSVYEILPAVLTLEKTMLYQ
                     QKIYRNGFR"
     gene            complement(2698..3411)
                     /locus_tag="PLW08_07200"
     CDS             complement(2698..3411)
                     /locus_tag="PLW08_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPL52424.1"
                     /translation="MNIGGLQKFSLLDYPGQLSAIVFTQGCNFRCPYCHNPELVDPQR
                     YSPLLETEKVLRFLYRRHKKLSAVVVTGGEPTLQEDLIPFLKLIKALRYKIKLDTNGS
                     RPEVLREIVNQGLVDYFAMDIKAPLKLYKVITRADLEVGTIEKSMELIRRSGVDYEFR
                     TTYFDLLLSTDDLASMQDLLKPGDKFIIQQCHYGKTLDEIKREDIVTGSFSFNENPAC
                     LSLIHWGTEHSIKISLRSL"
     gene            complement(3421..5547)
                     /locus_tag="PLW08_07205"
     CDS             complement(3421..5547)
                     /locus_tag="PLW08_07205"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPL52425.1"
                     /translation="MFSSIRKRNGSIVPFKQQKIAKAIESAGLATGEFGKDIAGVLTL
                     RVLNLAQQVIQDEIPEVEKIQDIVEEVLLASPYKKTAKAYIIYRDQHSRIRELVSKAG
                     VELIDQYLEKLDWQVNENSNMAYSLQGLNNYIASEVSKTYWLNKIYPPEIREAHLSGD
                     FHIHDLGQLSVYCVGWDLMDLLLTGFCGAEGKVESTPAKHFRSALGQIVNFFYTLQGE
                     AAGAQAFSSFDTLLAPFIYYDRLSYKEVKQALQEFVFNINVPTRVGFQTPFTNITLDL
                     KPHQIYKDQAVIIGGKPRDKNYGDFQAEMDILNRAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFEWDNPNLEYLWEVTAKYGIPYFSNFVNSDMDPKDARSMCCRLRLDTRKLETRG
                     GGLFGANPLTGSIGVVTINLPRIGYLAKTEEEFFQLLEERLLLAKKSLEIKRKVLENF
                     TAGGLYPYTKFYLKSIYERFQQYWKNHFSTIGIIGMNEAMLNFMGENLGTKKANAFAL
                     KIMDYLRKRLLEFQEETQNNYNLEATPAEGTSYRLALLDRKHFPDIICANCDADSPFY
                     TNSSQLPVNFSDDIFEVLDLQDELQTKYTGGTVLHIFGGERVEYGQSIKALVKTICEG
                     YRLPYFTFSPTFSICPQHGYLDGEQPICPLCQKKTEVFSRIVGYLRPVSQWNEGKQAE
                     FKMRTNFDLQKSKKKDSSAIAKSHFA"
BASE COUNT         1685 a         1129 c         1006 g         1753 t
ORIGIN      
        1 ctctttgtga aataagaagt tacagtcgga ttgtctcaat cgttccctgg ttaagtttta
       61 gagctggctc tccgtgaatc atcttgatga ttcccagttc tttaccacat tttggacact
      121 tgggtcgaga aaatgtttct gcgcctttca gtcctacaaa atccgcagtt aatctactta
      181 ttatgcattt gatcaggcgt ccctttcctg ctttgtaata acgaaaaagg gtgtatccac
      241 agcggcattt aacatcaatt attttgcctg ttttcattta gcgaaagccg ttacggtaaa
      301 ttttttgctg atagagcatt gttttttcca gggttaaaac agcaggcaga atttcatata
      361 cagaagggct ttccagcaat ttttccagtt gcagttcgtt accgattagg tttatggaaa
      421 cgataatctc tttcagatca tagtttttca ctttaggagg agtgcctttt gtgtaagtaa
      481 atatcttact tagcttaaag aatttgattt tatctcttat atgcaaatat aaagagtcag
      541 ggaaaatagt gctgagcaat tcagttcttg gcggagtaat ttttgttttt atgggctcaa
      601 cggcagttac agtaaaatca ggaattttgg ttttgttaaa ttcctgcaaa attagttccg
      661 ctgaataggg ttgataaaaa gagagatcaa aatattctgc caaaccctca attccaactg
      721 ctaaaggagg acataaagaa accttgggat ggggattaaa cccttgtgta aagatagttt
      781 tcagttctaa aactgatatt ctgcggaaaa gcatacgcat ccaatccaaa tgagaaataa
      841 agcgtaaaag tcccgttttt tgataataaa cccgatattt atattgagat gcttgataaa
      901 gcgggatttt cttattctgc tgcaagttat ctgtttctaa caaactacct tttgctgttt
      961 tagtttgaac ttcctcattg caaattccgc aggaagaaca aagctctcta caatcgggtg
     1021 tttgaattcc cgcttttgct ttttcccatt cggaaagcag aaaattctta tctataccca
     1081 gggaaataaa atcccagggc aatttgtctt gcggattgcg tgaacccaaa taactattta
     1141 agttaatgtt acattcaact attgcctcct gccaccagga aaaattaaaa cattcattcc
     1201 aaccatcaaa acgggcaccc ttgttaaaag cgagttcaat aacttccgct atttttgtgt
     1261 caccccggct aagtaatgct tccagaatgg aattttcaat ggtatgatag cggattttga
     1321 tatttttagc tttagcaaaa gcggatttga ttttgattgc ctttttcaat aatgattctg
     1381 catcttccat agcacaccac tgaaaaggag taaacggttt ggggacaaaa ggggaaagag
     1441 ttacatttat ttgtagacgc ctttttccct gttcattgat tttatggata agcttaataa
     1501 ttgcttcaat atcgtcttcc gtttcacttg gcaaaccaag cataaaatag agcttaatct
     1561 tttgccagcc aaattttaat gcggtttcaa ttccagccaa gatttgttct tcgctcaagt
     1621 ttttatttat cacctggcgt aaatgttcag agccggcttc aggagctata gttaagccct
     1681 ctcttcccaa acttttaagc gcttgaccga tttccatatc caaagtgtcc actcttaaag
     1741 agggaagagc aatatgggtt ttttgggtct caataaattt cagcaactgt aatagcaaat
     1801 tttgaatgca agaataatca ctgctggaaa gggataacaa gccaacttcg tcccaaccgc
     1861 ttgcttttat ttcttttagg atattttcta tgatagcatt gggttcgcgt tcacgcacag
     1921 gacgataaaa ataaccggca aaacaaaatc ggcaaccccg ggagcaacct ctcataattt
     1981 cagatacata gcgattatga gtagctaatt gccaggaaag taattgagga ctgtgttgtg
     2041 atttagatgt atgaaaagca gtatacttac gacaatgaat atgatcaatg ccattatgca
     2101 gagcaggaac ataacaggat ggtagtttgg aaatttgttt aattcgttcg gaacgcttct
     2161 tttcgttttt tagaatttcg gcaatttgaa tgataccttc ttcggcttcc ccgataaaaa
     2221 acacatctat aaaaggagct aaaggtaaag gatttacggc acaaggacct cctgccagaa
     2281 ttattgggtc tgtttcttgc cgttgcttgc tgtgtatatc tattccgctt aacttaatca
     2341 gttccagcac attagtgtaa ttaagttcac tttgcagggt aattcctaag atgtcaaagt
     2401 ccttaacctg ccttctgctt tcccaaccga aaagttccaa ttcctcttcc ctcattaaat
     2461 caatcaaatc aatccaggga agataaaccc tgtctgccat agcataatcc aattcattga
     2521 tcagggaata gagtattttt atgcccaaat gtgaaattcc cagttcataa gtatcgggaa
     2581 aagcaaaagc gaaatggact tgaaaggtgg aaaaatcctt tctccaggca ttaatttcat
     2641 tgtctatata tctggatggt ttttccacaa aaggcagaag gtgctcaata ttaactttca
     2701 taaactgcgc aaagatattt ttatgctatg ttctgtgccc caatggatta acgatagaca
     2761 ggcaggattt tcattgaaag agaaagaacc ggttactata tcttctcttt tgatttcatc
     2821 cagggtcttg ccgtaatgac attgttgaat gataaactta tcgcctggct tcagtaaatc
     2881 ttgcatagaa gcaaggtcat cagtgcttaa aagcaaatca aaataagtag tgcggaattc
     2941 ataatcaact cctgatcggc gaataagttc catacttttt tctattgttc ctacctccaa
     3001 atccgctctg gttataactt tataaagttt caacggggct tttatgtcca tcgcaaaata
     3061 atccaccagt ccttgattaa cgatctcccg taagacctca ggacgagaac cattagtatc
     3121 caattttatt ttgtagcgca gtgcttttat taactttaga aaaggaataa ggtcttcttg
     3181 caaggttggt tcacctcctg taactactac tgcggaaagt ttcttatgac gacgataaag
     3241 aaaacgcaga accttttctg tttccagcaa ggggctatat ctttggggat caacaagttc
     3301 gggattatga caataaggac agcgaaaatt gcagccctgc gtaaaaacaa tggcagataa
     3361 ctgccccgga taatccaaca gcgaaaattt ttgcaaccct ccaatattca ttatctggtc
     3421 ctaagcaaaa tgacttttgg caattgcgga actatctttt ttcttgctct tttgcagatc
     3481 aaaattggtg cgcatcttaa attcggcttg cttgccttca ttccattgag aaacgggacg
     3541 taaatagcca acaattcggg aaaaaacctc tgtctttttt tgacagagag gacaaattgg
     3601 ctgttcacca tccaaataac catgttgagg acaaatactg aaggttggag aaaaagtaaa
     3661 atagggcaaa cggtaaccct cacagattgt ttttaccagg gcttttatgc tttgtccata
     3721 ttcaactctt tctccaccga agatatgcaa aaccgttcca ccggtatatt tggtctgaag
     3781 ttcatcctgc aaatccaaaa cctcaaagat atcatccgaa aagtttacag gcaattgact
     3841 gctattggta taaaaaggtg aatccgcatc acaattggca caaatgatat cgggaaaatg
     3901 ttttctatcc aaaagcgcca aacgataact tgttccttcg gcaggagttg cttccagatt
     3961 ataattattt tgggtttctt cctgaaattc cagtaaacgc ttacgcaggt aatccattat
     4021 ttttaaggca aaggcattag ctttctttgt tcctaagttt tcacccataa agtttaacat
     4081 cgcttcgttc ataccgataa ttccgatagt ggaaaaatgg tttttccagt attgctgaaa
     4141 acgctcataa atgcttttca gatagaactt ggtgtaagga taaagtccac cagcggtaaa
     4201 attttccagc accttgcgtt ttatttccag ggattttttt gccagcagta aacgctcctc
     4261 caaaagctga aaaaattcct cttccgtttt tgccagataa ccgatacgag gtaaattaat
     4321 agttaccact ccgatggaac cggttaaagg attagcccca aaaagtccgc cacccctggt
     4381 ttccagtttt ctggtatcca aacgcaaacg acaacacata ctgcgtgcat ctttggggtc
     4441 catatcagaa tttacgaaat tggaaaaata gggaataccg tatttggcag ttacttccca
     4501 taaatattcc agattaggat tatcccattc aaaatcctta gttatattat aggtaggaat
     4561 tggaaaagtg aaaacccgcc ctttggcatc accttcaatc attacttcca gaaatgctct
     4621 gtttaaaatg tccatttctg cctgaaaatc accataattt ttatcccttg gtttgcctcc
     4681 aatgataact gcctgatctt tataaatctg atgcggtttc aggtctaaag taatattggt
     4741 aaaaggggtc tgaaaaccaa ccctggtagg aacattgatg ttgaaaacaa attcctgcag
     4801 tgcctgtttt acttctttat aacttaaacg gtcataataa ataaagggtg ctaaaagagt
     4861 atcaaaactg gaaaatgctt gtgcgcctgc tgcttcaccc tgtaaagtat aaaagaaatt
     4921 tacaatttgc cctaatgcac ttctaaaatg tttggctgga gtactttcca cctttccttc
     4981 cgcaccacaa aatcctgtta aaagaaggtc catcaaatcc caacctacac aataaacaga
     5041 aagttgaccc aaatcgtgaa tatgaaaatc cccgcttaaa tgtgcttccc tgatttccgg
     5101 aggatatatt ttattcagcc agtaggtctt acttacttcc gaagcgatgt aattattcaa
     5161 tccctgcaga gaatatgcca tattggaatt ttcattcacc tgccaatcca acttttccaa
     5221 atattgatct atcaattcca ctccagcttt ggaaacaagt tcccgaatcc gagaatgctg
     5281 atcacgataa ataatatatg ctttggcggt cttcttataa ggtgaggcaa gcaagacctc
     5341 ttctacaata tcctgaatct tttccacttc aggaatttca tcttgaataa cctgctgtgc
     5401 caaattcaaa acccgtaagg ttaaaacccc ggcaatatct tttccgaatt cacctgtggc
     5461 aagtcctgca ctttcaattg ctttggctat tttttgttgt ttaaaaggaa cgatagaacc
     5521 gtttcttttg cgaatagatg aaaacatctc caaacctcta ttttcttaag tca
//
LOCUS       DAOOZV010000088         5325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_13169_length_5325_cov_70.659203, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000088 DAOOZV010000000
VERSION     DAOOZV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13169_length_5325_cov_70.659203"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..377)
                     /locus_tag="PLW08_07210"
     CDS             complement(174..377)
                     /locus_tag="PLW08_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52426.1"
                     /translation="MSREKEIIFHKEKREKEIVFTKRKRDYFHKEKREKEKKRKRYYF
                     HKEKREKRLFSQREKEKREKYKF"
     gene            459..1181
                     /locus_tag="PLW08_07215"
     CDS             459..1181
                     /locus_tag="PLW08_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPL52427.1"
                     /translation="MIGERLKQIRQVLGIKQVDLAKVLKINPSAISQMESGRTNPSLE
                     TLSELVVNYNVNLHWLITGIGKMFNTANDTSSQNGSWDNFQKLLNDRLEEIVQAHLDL
                     MDSDTVEIPVSGEIAAGEPMENYGTLLDVVTVRRSLINGSLNNFVALRVNGRSMEPDI
                     RNQDVVLIRYCNEWRELAGKICAVRIDGGITLKRLILDDVQKMIVLLSINEKYQPILI
                     DPDSHTDVTLIGSLYFLFRILP"
     gene            1196..2575
                     /gene="rmuC"
                     /locus_tag="PLW08_07220"
     CDS             1196..2575
                     /gene="rmuC"
                     /locus_tag="PLW08_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPL52428.1"
                     /translation="MTLVYLLIIVAIVFAIVNLYILLSQQHKGKENGNISELKGAFET
                     FERYNREEMSSLRTELLSISAENRKELSDSIKAQNEVLIAQTTASREELNNSFNALKQ
                     QINNDSAVNREEQKKALNDLSENFTRRMTDLINIQQQEAETSRKTLETKMEQIRQNNE
                     TKLEQMRQTVDEKLHDTLEKRLGESFKQVSERLELVHKSMGEMQSLANGVGDLKKALT
                     NVKRQGVLGEIQLANLLEDILTPEQYEKNFRPNKNRDEKVEFAIRLPGNSEKEEFVYL
                     PIDAKFPIADYQNIMNAYDLGDLAEVENARKSMVQRIKNCAKDIRDKYINPPVTTDFG
                     MLFLPFEGLYAEVLRSPGLFETIMRDYHVIICGPTTTAAVINSLQVGFRTLAIQKKTS
                     EVWKILASIKLEFGKFGTILEQTQKKLQEASNNIEKASHRSRQIEKRLDKVQELPVEE
                     AEIEQILDV"
     gene            complement(2669..3337)
                     /locus_tag="PLW08_07225"
     CDS             complement(2669..3337)
                     /locus_tag="PLW08_07225"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPL52429.1"
                     /translation="MLCVNNNRAYIFTNHAPQDIVSHYKNIQRKDHLIAVDNGIKKID
                     ELGLEPFVLIGDLDSIEPEMLEKYAYIPFHQHPTEKNETDTELALDWCLKQQCYREII
                     ICNDMQGRFDHALAIAINLLKIKSPRGERISYPVPYRIESESQSIFILPDSIEFNNCQ
                     GKLLSLIPLSEKVVFSWSQNLKYPLNDLVIRDYQSRGISNIITASSAGIIKTEGICLA
                     VLEG"
     gene            complement(3324..4223)
                     /locus_tag="PLW08_07230"
     CDS             complement(3324..4223)
                     /locus_tag="PLW08_07230"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL52430.1"
                     /translation="MENEFINKGKMKISYQVGMDGVKHSFTLPHYAFILIIVLLAFLL
                     VSTIIMFTMAGSNSHRNEVLKRLETENKELRAKLDFYSATVDSIYKRLNELQIKINEN
                     SENYPALNFKQGKKADFVFEPPLRNQMENLETKLATILVLISEPAKANRALPTGENPA
                     DYIPSIYPTFGRISDGWGLRVHPIRNEIEFHYGLDISNEAGTPIYATAAGTVVTTDYE
                     TSYGKRIIINHENGYQTLYGHLYSYMVRSGDQVIKGQIIGLMGSSGISTGPHLHYEVH
                     NNLGKVNPVAYLNRIDEPLYVMR"
     gene            complement(4290..4365)
                     /locus_tag="PLW08_07235"
     tRNA            complement(4290..4365)
                     /locus_tag="PLW08_07235"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4329..4331),aa:Glu,seq:ctc)
     gene            complement(4398..4472)
                     /locus_tag="PLW08_07240"
     tRNA            complement(4398..4472)
                     /locus_tag="PLW08_07240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4437..4439),aa:Glu,seq:ttc)
     gene            complement(4479..4553)
                     /locus_tag="PLW08_07245"
     tRNA            complement(4479..4553)
                     /locus_tag="PLW08_07245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4519..4521),aa:Lys,seq:ttt)
BASE COUNT         1642 a          929 c         1061 g         1693 t
ORIGIN      
        1 tgcccgtttt ttattttaag ctgattttta gccaccgaaa acaccgaaag atttatgcac
       61 acagattaca cagattttta gccaccgaaa gcaccgaaag caccgaaatt tatatataat
      121 tataaccaat aatattagtt cgtagttctg agttcgtagt tcgtagttgc ttattaaaac
      181 ttatattttt ctcttttctc tttttctctt tgtgaaaata atctcttttc tcttttctct
      241 ttgtgaaaat aatatctttt tctctttttc tctttttctc ttttctcttt gtgaaaataa
      301 tctctttttc tctttgtaaa aacaatctct ttttctcttt tctctttgtg aaaaataatc
      361 tctttttctc ttgacaaatt tagtggcact aataatgaag gtttaagatg ttaatattac
      421 tcatttctaa gtgtagctaa catcggaagg agataattat gattggagaa cgacttaaac
      481 agattcgtca agtattggga atcaaacagg ttgatttagc caaggtctta aagattaatc
      541 cttccgctat ttctcaaatg gaaagtggca gaacaaatcc ttctctggaa acgctttcgg
      601 aattggtggt aaactataat gtgaatctgc actggttaat aacgggtata ggaaaaatgt
      661 ttaatactgc taatgataca tcttctcaga atggttcttg ggataatttt cagaagctct
      721 taaatgaccg tttggaagaa attgtgcagg cacatttgga tttaatggat tccgacactg
      781 tagaaattcc ggttagtgga gaaattgcag cgggtgaacc aatggaaaat tatggtactt
      841 tattggatgt tgtaacagtg cgccgttcct tgatcaatgg ttctttgaat aattttgtgg
      901 ctttaagagt gaatggacgt agtatggaac cggatattag aaatcaggat gtggtgttaa
      961 tccgttattg taatgaatgg cgagagctgg caggcaagat ttgtgcggtg cgtattgatg
     1021 gaggaataac attaaagcgt ttgattttgg atgatgttca aaagatgatc gtattgcttt
     1081 ccataaatga aaagtatcag ccgattctga ttgatcctga tagtcataca gatgttacac
     1141 taatcggttc gttatatttt ttattcagga tattacctta aggagggata agtaaatgac
     1201 tttagtttat cttcttatta ttgtagccat cgtctttgct attgtaaatt tatatatttt
     1261 actttctcaa cagcataagg gtaaggaaaa tggaaatatc agtgaattga agggtgcctt
     1321 tgagaccttt gaacgctata atcgtgaaga aatgagcagt ttacgaactg aacttttaag
     1381 tatcagtgct gaaaacagaa aagaacttag tgactctatt aaagctcaaa atgaggtctt
     1441 aatagcacaa acaactgcca gcagggaaga actgaataat tcctttaatg ccctaaaaca
     1501 acagattaat aacgattctg ccgttaatcg ggaagaacag aaaaaagcat tgaacgacct
     1561 ttccgagaat tttacccgca gaatgacaga cctgataaat attcaacaac aggaagcaga
     1621 aacatcgcgt aagacattgg aaactaaaat ggaacaaatt cgtcaaaata acgagaccaa
     1681 actggagcaa atgcgtcaaa cggtagatga aaaattgcac gataccctgg aaaagcgttt
     1741 aggtgaaagt tttaaacagg ttagcgagcg tttggagctg gtacataaaa gtatgggtga
     1801 aatgcaaagt ttggcaaatg gagtgggaga ccttaaaaaa gcccttacca atgttaaaag
     1861 gcagggagtt ttaggggaaa tccaattggc gaatttactg gaagacattc ttactccgga
     1921 gcagtatgag aaaaacttcc gacccaataa aaaccgtgat gaaaaagtag aatttgccat
     1981 tcgcctgcca ggaaactcgg aaaaagagga atttgtatat ctgcctatag atgccaagtt
     2041 tcctatagcg gactatcaaa atattatgaa tgcttatgat ttgggtgatt tggcggaagt
     2101 ggaaaatgcc agaaaaagta tggtgcaaag gattaaaaat tgtgccaaag atatacggga
     2161 taaatatatt aatccacctg ttactactga tttcggaatg ctttttttac cttttgaagg
     2221 tctttatgct gaggtcttac gcagtccggg actttttgag acaattatgc gagattatca
     2281 tgttatcatt tgcggtccaa ctaccactgc tgcggtaatt aacagtttgc aggtgggttt
     2341 tcggaccttg gcaattcaga agaagacctc agaggtttgg aaaatccttg cctccatcaa
     2401 actggaattt ggtaagtttg gaactatttt ggagcaaacc caaaagaaac tgcaagaagc
     2461 atcaaataat attgaaaaag caagtcaccg cagccgtcaa atagaaaaac gactggataa
     2521 ggttcaggaa ttgcctgtgg aagaggcaga aatagaacaa attctggatg tttgattggc
     2581 ggaagaacaa catcctgtct tgctattttt tcttttataa gttgacaagg acatcaactt
     2641 tccgggcaaa caaggttgac ggaaaacatc aaccttccag gactgccaaa caaatacctt
     2701 ctgttttgat aattccggct gacgaagcag ttataatatt actgattccg cgtgattgat
     2761 agtcccttat tacgaggtcg ttcaggggat atttcaggtt ttgggaccag gaaaaaacaa
     2821 ctttttcgga gagtgggata agagaaagca attttccctg gcaattgtta aattctatgc
     2881 tatccggcag aataaaaatg gactggcttt cactttcaat cctataagga acagggtagg
     2941 atattctttc gcctcttgga ctttttattt ttaacaaatt tatagcaata gccaaagcgt
     3001 gatcaaagcg accttgcata tcgttgcaga taattatttc ccgatagcat tgttgcttta
     3061 aacaccaatc cagtgccagt tcggtatccg tttcgttttt ttccgtagga tgctggtgga
     3121 aggggatgta agcatatttt tcaagcattt cgggttcaat ggaatccagg tctccaatca
     3181 gaacaaaagg ttctaatcca agctcatcta ttttttttat gccgttatca acagcgatta
     3241 aatggtcttt tctttggata tttttatagt gggaaacaat atcttgggga gcgtgattgg
     3301 taaaaatata agcgcggtta ttattaacgc ataacataga gaggttcatc tattcggttc
     3361 aaataagcaa caggatttac ttttcccaga ttattatgca cttcataatg caaatgagga
     3421 cctgtagaaa ttcccgaact gcccatcaga cctataattt gaccttttat aacttgatca
     3481 ccggaacgca ccatatagct gtagagatga ccgtaaagcg tttgatatcc attttcgtga
     3541 ttgattatta tccttttacc ataactcgtt tcataatcgg tagttaccac agttccggct
     3601 gcagtggcat aaattggagt tcctgcctca ttggaaatat caagaccata atggaattca
     3661 atttcattcc gaataggatg aactcgcaaa ccccatccgt ctgaaattct gccgaaagta
     3721 ggataaatag aaggtatata atcagcagga ttttcccctg taggcaaagc acggtttgcc
     3781 tttgccggtt ctgaaatcag caccagaatt gtagccagct ttgtttccag gttttccatc
     3841 tgattgcgca aaggcggttc aaaaacaaag tccgcctttt ttccctgctt aaaattcagg
     3901 gcaggatagt tctctgagtt ctcattaatt tttatttgta attcatttaa tctcttatag
     3961 atagaatcta cagtagcgga gtaaaaatca agtttggcac gtagttcctt attttccgtt
     4021 tccaaccgct tgagaacttc gttacgatga gaattgctac ctgccattgt gaacataata
     4081 attgtggaaa caagcaaaaa tgccaaaagc acaattatca aaataaaggc ataatgaggc
     4141 agggtgaagc tgtgtttaac tccatccatt ccaacttgat agcttatttt cattttacct
     4201 ttgttgataa attcgttttc cactggcgtc cttttctctt atcttttttc tttctatatt
     4261 atgcaaaatg cgaaccaaaa atggttattt ggcatcccgt aggggaatcg aacccctgtt
     4321 gcgtgactga gaatcacgag tcctaggcca ctagacgaac gggacacaaa ttgtttcctt
     4381 atttttaccc tataaattgg cgacccctag gggaattgaa cccctcttgc aagaatgaaa
     4441 atcttgagtc ctaaccaata gacgaagggg tcgcaaactg gtgatccagg ttggaatcga
     4501 accaacgact ctctgcttaa aaggcagata ctctaccgct gagttactgg accttttttc
     4561 tgcttatctt ttaactaaaa atttagaggt gtgttctatg tcaagtaaaa tcgcatttat
     4621 tttttaacac cgagatcact gaaggcatta tgcgttctca cttatttttt caaatcggcg
     4681 tttttgctgg cttgcgatat catgattatt tcaaatattt tttgtatggc attgtataac
     4741 ttgcgattat ggccggcact ctttttattt ttccgagaag aaattccgta gaaacgacag
     4801 gttttaacga cgggttttaa cccggcgtta tgaagatgga agatatctta tttccttttt
     4861 gcgaatgagt tccgtagtaa attggaagtt gccaatgcat ttgttctcaa ctgcattttg
     4921 ggggttaagt aaagtccatt gttttcagag catttcagct ttttccagct tgaattgctg
     4981 aagcattgct tacccattac ggatgggatt agcaatgggt aagcaatgag taagcaatgc
     5041 tattaaggaa gaaaatggaa ttatatttat ttagtaggag ttaggaaaat ttagggggaa
     5101 gaagaaaata atatatctgt aactccatat tttatatggt tttaatattc ataaactcta
     5161 tataataaga taaatcctat agtgatggca gttagataac gacgagtttt aaccccagca
     5221 catattctat tttaactcac aattttcacc ggggggaaag ttttgagaaa gttttgactc
     5281 acaattttca cttgccctcc aggttcacac atcccacaaa catct
//
LOCUS       DAOOZV010000089         5147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_13706_length_5147_cov_65.441477, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000089 DAOOZV010000000
VERSION     DAOOZV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13706_length_5147_cov_65.441477"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1129)
                     /gene="dprA"
                     /locus_tag="PLW08_07250"
     CDS             complement(41..1129)
                     /gene="dprA"
                     /locus_tag="PLW08_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPL52431.1"
                     /translation="MERYKAWLCLKSAPLLGSKGTLELLQSFPDPQEFVGNPSHPLYQ
                     NGKLKPPTIKHLSEFILPEDNKRILTLMEKHQLQYLCLTDPDYPAALKEIFDPPLILY
                     YQGDLTGTLKDLCIAVVGTRKPTSYGLEMCRKLLSPLCAKGVNVVSGLAMGIDTIAHQ
                     TALKAKAKTIAVFASGLDNIYPPMNKTLAENICENGALVSEYEPGSKLERWNFPSRNR
                     IISALANAVLVIEGSLNSGAMLTAKFAIEQNKPLYALPGNINNRNAQGPNLLIRQGAT
                     LISSANDLLMDLGLVASPKEQMEIIPELSSEEQTLYDILSSAQKEITFDEFMLKTGLG
                     FGKLSTLLLNLELKGLIAKSSGNSYIKL"
     gene            complement(1130..2140)
                     /gene="obgE"
                     /locus_tag="PLW08_07255"
     CDS             complement(1130..2140)
                     /gene="obgE"
                     /locus_tag="PLW08_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPL52432.1"
                     /translation="MFIDYARIKIKSGNGGDGTISFRREKYVPKGGPDGGDGGRGGDI
                     IAVGDSNLNTLLDYRYHKFFKAGNGKPGAGAKKTGSSGANCILRLPLGTEIYVLEENK
                     KYKLADITQAGEKVILSAGGSGGKGNYNFATPTNQAPRIATPGVKTEEMELELVLKLM
                     ADVGLVGFPNAGKSTLLSVLSSARPKIADYEFTTLEPMLGVVYIGDYQNFVMADIPGI
                     IKGAHLGKGLGDQFLRHIQRTHLLLFLIDIATPDPLEAYRTLRSELYLYDSFMDKKPH
                     LIVISKTDTLSPEDLKQKLTEIRNAFKKEYNDEIFAISAVSKDGLDELKYKIYNILQQ
                     SR"
     gene            complement(2149..3627)
                     /locus_tag="PLW08_07260"
     CDS             complement(2149..3627)
                     /locus_tag="PLW08_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52433.1"
                     /translation="MKHILTVILVGITTLLMAQYEIPFGNIRHSSRDENGYIHLRWED
                     LSEGTLNPEFYYSANDAPWQNSTIYDISAGEKEALAPYSFNQKLRYRLHYSIEEMGET
                     VAMMHSAYWDSDTFPPQLNKMAYIGTDAEGDSVTVYSQNLDLRESYTAISSDKIYFTM
                     KNCSGSYPIMNSLTSYNVYLSVLGNITSLIDSVGYAMVYSFNIPGLISSGLYKVGYDS
                     STQQPVFTRLGNVQSQVSGGALNLACNISDLTTDPNFGALPDAIMMLGLTLKVDMDLA
                     NLEPQIGLGDYSTPAFIIFQDNYYQVSQNTLPVCTFNGYNPSTGILLIDYWDADADFP
                     LISEAILPDSTILQFLPTGLDYSFPVTYTLQLPVIEVDSLIWRFSDNGFDIVQDVVHF
                     VGNQDGYLIPSLLSCTMPNPVYSLPVNINLKGLCNCPVSIEIYNIKGQKVAEINQPPT
                     GNGELNINWKGMVSGKQLSSGVYFMKVDNGKQTLKRKFVVAK"
BASE COUNT         1621 a          985 c          914 g         1627 t
ORIGIN      
        1 tcggtttaga gtcaaaacaa gaacccacta tttcgctatt ttacaatttg atgtaggaat
       61 taccgctgct tttagctatc agtcctttca attccaaatt cagcagtaaa gtagaaagtt
      121 ttccaaaacc gagacccgtt ttcagcataa attcatcaaa agtaatttct ttttgtgcag
      181 aagacaaaat atcatagagg gtctgttctt cggatgacag ttccggaata atttccattt
      241 gttcttttgg agaagcaact aagcccaaat ccattaagag gtcattggca gaactgatta
      301 aggtagcacc ctgtctgatg agcaaattag gaccttgggc attgcgatta ttgatatttc
      361 ctggcaaagc ataaagcggt ttattttgtt caatggcaaa tttggcagtg agcatagctc
      421 cgctatttaa agagccctca ataactaaaa cggcattggc aagagcactt attattctat
      481 tacgggaagg aaaattccag cgttccagtt tgcttccggg ttcatattca ctaaccagag
      541 ctccgttttc gcatatattt tctgcaagtg ttttattcat agggggatag atattatcca
      601 agccggaggc aaaaacggca attgttttgg cttttgcttt cagagctgtc tgatgagcta
      661 tagtatcaat tcccatagct aaaccactga caacattcac tccttttgcg cataagggag
      721 aaagaagttt acgacacatt tccagtccgt aggaagttgg tttacgagtt cctacaactg
      781 cgatacaaag gtctttcaag gttcccgtaa ggtcaccttg gtaatacaaa atcaaaggtg
      841 gatcaaatat ttcttttaag gcagcaggat aatcgggatc ggttaaacag agatattgaa
      901 gctgatgttt ttccattaaa gttaaaattc gtttgttgtc ttcaggcagg ataaattcgc
      961 ttaagtgctt gattgtgggg ggttttagtt tgccattttg ataaagcgga tgagagggat
     1021 tgcccacaaa ttcttgagga tcaggaaaac tctgtaaaag ttccagtgtt ccttttgaac
     1081 ctaaaagggg agctgatttc aagcagagcc atgctttata gcgttccatc taacggctct
     1141 gctgtaatat attgtaaatt ttgtatttaa gttcatctaa accgtctttg ctgactgcgg
     1201 aaatagcaaa aatttcatca ttatattcct ttttgaaggc attgcgtatt tccgtcaatt
     1261 tctgctttaa gtcctcggga gaaagagtat ctgttttgct gattacaatt aaatggggtt
     1321 ttttatccat aaaggaatca taaagataca gctccgacct caaagttctg tatgcttcca
     1381 aaggatcggg agtggcaata tcaattaaaa aaagcaagag atgagtgcgc tgaatatgac
     1441 gcaagaattg atctcccaat cccttaccta aatgcgctcc tttaattatt cccggaatat
     1501 cagccatcac aaaattctga taatcaccaa tataaacaac ccccagcatc ggttccaaag
     1561 tagtaaattc atagtccgct attttaggac gagctgaaga taacacactt aaaagagtgg
     1621 acttacctgc attgggaaaa cccactaaac ccacatcagc catcagtttc aaaaccagtt
     1681 ccagctccat ctcctctgtc ttaacaccag gagttgcaat tcttggtgcc tgatttgtgg
     1741 gagtggcaaa attgtaattt ccttttcctc cggagccacc tgcggataaa ataacctttt
     1801 ctccggcttg tgttatatca gccagtttat attttttgtt ttcttcaagc acataaattt
     1861 cagtgcctaa aggaagacgc agaatacaat tcgcacctga agaaccggtt ttttttgctc
     1921 cagcacctgg ttttccgttt cctgctttga agaatttatg ataacgatag tccaaaagag
     1981 tatttaaatt gctgtctcca acggcaataa tatctcctcc ccttcctcca tctcctccat
     2041 caggaccacc tttaggcaca tatttttcgc gtctgaaact gattgtgcca tcacctccgt
     2101 ttccggattt gatttttatt cgtgcgtagt caataaacat atctgctgtt actttgccac
     2161 cacaaatttg cgcttaagcg tctgttttcc gttatctact ttcataaaat atactcctga
     2221 agataattgt tttccggaga ccattccttt ccaattgata tttaattcgc cgtttccggt
     2281 tgggggctga tttatttctg ccactttttg tcctttgata ttataaattt ctatgcttac
     2341 aggacagtta cacaagccct tcaaatttat atttaccggc aaagaataaa ccggattggg
     2401 catagtgcag gataataaag agggaataag gtaaccatct tgatttccaa caaaatgaac
     2461 aacatcctga actatatcaa aaccgttatc actaaaccgc caaataagag aatcaacttc
     2521 tataacgggt aactgtaaag tgtaagttac agggaaagaa taatccaaac ctgtaggcaa
     2581 aaattgcaat attgtgctat ccggtaagat agcttcactt attagaggaa aatccgcatc
     2641 cgcatcccaa taatcaatca gtaaaattcc cgttgaggga ttgtaaccat tgaaagtgca
     2701 aacaggaagt gtattctggc tcacctgata ataattatcc tgaaagataa taaaggcagg
     2761 agtgctataa tcgccaagcc caatctgtgg ttccaaattt gctaaatcca tatctacttt
     2821 cagcgtaagc cctaacatca ttatagcatc cggtaaagca ccaaaatttg gatcggtagt
     2881 taaatcgctg atattacaag caagatttaa tgctcctccc gaaacctgac tttgaacatt
     2941 gcctaaacgg gtaaaaacag gttgctgtgt ggaagaatca taaccaactt tatacaaccc
     3001 gctggaaatt aaaccaggaa tattaaaact atagaccatc gcataaccta cactatctat
     3061 caacgaggta atattgccca acacggaaag atatacattg taactggtta gtgaattcat
     3121 tatcggataa ctaccgctgc agttcttcat tgtaaaatat attttatcgg aactaattgc
     3181 agtgtaggac tcccgtaaat ctagattttg agaatatacc gttacactat ccccttctgc
     3241 atctgttcca atataagcca ttttatttaa ttgtggagga aaagtatcac tatcccaata
     3301 ggcagaatgc atcattgcta cggtttcacc catttcttca atgctataat gaagtcggta
     3361 acgcaatttt tgattaaaac tatagggtgc caatgcttcc ttttctcctg cagatatatc
     3421 ataaatagtt gaattctgcc agggcgcatc attggcacta taataaaact ccggatttaa
     3481 ggtcccttcg cttaagtcct cccaacgcaa atgaatatag ccattttcat ctctggaact
     3541 atgacgaatg ttcccaaacg gaatttcata ctgagccata agtaaagtag ttatacccac
     3601 taaaataact gttaaaatat gtttcatcgc tacctcgcct tcctgaaact ggctaatacc
     3661 atatatattt gtaattcctg ctgttctctg tccttattga aaaagcagga atacattatc
     3721 cttctttgca aatgcttaaa ggcattaaaa tcactttacc tctattattt gtactattta
     3781 taatataata ctgtaaagcc cagcttatgt caagtatttt ttgctgttca ttttctacag
     3841 gaaaagttcc tgaccaaaga tgatatcttc aacaatagga ttttataaca gatttgccct
     3901 aatcatctta ttgagtgagt aatgaagagg tggtaattat ccaagcatag cttttaaaat
     3961 ctgatttgag atcatatagt gattatttac tacttttggc taaaaggata tttccccagt
     4021 cattcatagc agaatattac aagtatagag tttcaataca agaaacgaaa caagtagata
     4081 ttgattagtt tagaatgaga gctgtataat agaatattat ttattagttc tctggtttgg
     4141 ataacctgat aataggaaac tatctggttt caaaaaatag ctttgggaca gaaaaattct
     4201 aattcctaaa acatctaaac aagaaccaat tttaaaccta aatcacttca tcaaaataaa
     4261 aattgttcaa accgaattga gttttctatt atatagatat tgctattccg aaaagatcaa
     4321 taaaacatag gttttttatt gttgaaccaa gtggaatttc aaatcctaac agttttatct
     4381 tcaaatgttt tccctggcaa tctaatacaa ggggtttatt actccaaaac atgtcttgct
     4441 ggggattccc gcaattatat tttttgtcta ttagatccaa tagatccggt tatctgcctc
     4501 ctatttcttt tgccaccgca acaacaagtt ttaactccag tactacaaac taagaacttt
     4561 tcgcttaagg gtatcataaa tcctcaaggc agcagatgcg gtataattgt caaaaagttc
     4621 ttgacaatat aaataggata tttattagtg tctttatgaa aagtgaaggt ttctgccgaa
     4681 attatggaag agagataaag gactgaagag aaaaggagtt tatgaaggtg aaaaacatca
     4741 gcttaaagtt aaattcctcc aagaggaatg gtttttttgc caggcaagag aagaatgata
     4801 gtgtgttctt gatgaagaaa tatagatata tttggttaag gagcgtagta acaggtttat
     4861 tccttattta cttacgatta tcaaaagatg ttatttcggt tccgctcttg ttgttcagga
     4921 aaagtgatca ttctcactgg agcggtgtat caataaagca atcaggaaaa caatttttta
     4981 ctactaccta ctaataaggg agaataaaat gaaaaaatca cttgtcgttc tgcttattgt
     5041 tttgacaatg agcggttttc tctttgctgc ggagtatacc attgtagatg gtagctccaa
     5101 tagttatcat attcccgtta acggaaattc caattatgga tggagcc
//
LOCUS       DAOOZV010000090         5128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_13768_length_5128_cov_56.351469, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000090 DAOOZV010000000
VERSION     DAOOZV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13768_length_5128_cov_56.351469"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2832
                     /locus_tag="PLW08_07265"
     CDS             <1..2832
                     /locus_tag="PLW08_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52434.1"
                     /translation="HNREYFEANLEALKYYYLAVSAGAYELHYDVYPKNIHAYTLPKS
                     MGYYNPPGASSELFVSRMEEYFKTAFETADSIDEEIDFASYGHYMIIHSGSDWQHDIM
                     GDTPSDLPSFFIKVGDGKEAVVDNGTVLISHSCNVPETISQDFYENEENGTIIHSGYG
                     ALNAVIAHEFGHSLGMVDLYNVYNSSPMVGVFDIMDSGGSGILIDELDNGDLVYVEGA
                     LPTLPGAFSRNLMFRDSYLERGLLKEFPDFNTMTELSLSAISAMQNGNDLVPHTYKIP
                     LNSKEYILVENRNVDPDGDGGTAVFGALDGRVILYPTPFEDISPPPPTYEYDYLLPSF
                     MKANGSAIGGGILVWHINEAILYDEGQTDSSGNWTSNFDANTVNTGFYHRGVSVIEAD
                     GLTDLGSDYSMYWTGTPYEYFHKYKPILDTNGLFVNWSQEPWKPELTAVTKPPLVDSF
                     NVPCLYHLKEIGNPAKKMSFKVESGSFDTTQIFDYSTSSFIIGPIIKTGFNEDNIPVL
                     IDNKMFLLSYWDNEWQDIIGEFVTNFPRSDYPLQKVDLNTDGYFELVWAKQNTLVLVD
                     WSLDTPIFHQISFPDSITTTPLPVNNTLIVTTKDCIYTVKNFAIQNFISLQGAKCIAG
                     YDDKIIVLGTDFLAALTLDNLDKFNEFTLPEPCGNIEPLIYTNPDKTIEMIFVMANSG
                     NLYRIYQNRLELIFANHSKEKPGQLACSSLGDISPVLFFGIGNKQYALKADGTKLTHF
                     PVIAPKAISPCETPFALKNGSAEILFYPLSETGYLAIDKTGRIVPEMCLSVNKDKKND
                     YLYYQPEQKILNWYYPDSTGRFFIHSKKNIDTNPILFAGYRNGASGCATFAFRDESIS
                     STEKIAYIYPNPVRKPYYKLNLQNYFGETKLHLYDISGTLVKKMIIPESNNNPRDFEL
                     NTLGLSSGVYIMVLENNGSTKRLKFAVEK"
     gene            2852..3820
                     /locus_tag="PLW08_07270"
     CDS             2852..3820
                     /locus_tag="PLW08_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52435.1"
                     /translation="MKKFILLIFIAGFAFCLFSQEKPKTDIDQWLNQTEEEQQNAMRD
                     SVVTNDLIKINYNRKNAQLAMAMSMLVPGAGQFYANKTVLTTYIFPVLEIGLIGGLVY
                     FNNQGKDKTKKYEKYANGEIVTYITPDGTKITGFRYDRSRQDEIQAILIGVNQHDIYD
                     NGYFRLDKTNSQHFYEDIGKYPQYVFGWMDWYYRFACDESGVRDSIRWKFDGENDSPN
                     MRWTGNYPLWGNDTETLIPIDSHEMSRMRQTYIKMRNDAKDSYSMAHFFTLGLAFNHL
                     AAGLDAIRVTRNFNRYYISQSVPTLNFYAAMPLGNVTPMLGLKWTF"
     gene            3817..4467
                     /locus_tag="PLW08_07275"
     CDS             3817..4467
                     /locus_tag="PLW08_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52436.1"
                     /translation="MKRKTCLIILLLLWGIIPLLALPKFVYPVSSLILPGTGELLLGH
                     NTRGATLMGVDLINIYAFIATDREIEMQKKNFMKFAELYAGVPYGMPDEHYQTVQNYP
                     NSDYYNDIQEMMARNYYLIYNYDPESFAEYISANTYQGNEEWSWQSEEKWEEYKHLRK
                     RHQKTKMNNQLALGLMLLNRGISIIDSSILSKKKHGELYLAPLPANGAMLNYQIEF"
     gene            4478..5125
                     /locus_tag="PLW08_07280"
     CDS             4478..5125
                     /locus_tag="PLW08_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52437.1"
                     /translation="MKRFGIFLLIILLSTSLFAQSRGILTLKSFAVPGWSQISSNRNY
                     GYAMLTVEAVIIGSIYYFHSESKILEQECYEYAIKFAHLNPDGYSDEFYKQLARYESS
                     GFDAGGYNNWVRQTAMNLYPDDPESQQQYINENAYGNDKYWYWDSSNHRSEYNKIRNR
                     SMDYDNYALVAGGILILNHLVSTIDVLRWTAPPKKANLSFNLQDKTPILQINYHW"
BASE COUNT         1606 a          876 c          996 g         1650 t
ORIGIN      
        1 cataacagag aatattttga ggcaaatctg gaagcattga aatattatta tttagctgtt
       61 tctgccggtg cttatgagct tcattatgat gtctatccca aaaatataca tgcctatact
      121 ttgccaaaaa gtatgggtta ctataatcct ccgggagcca gttccgaatt atttgtcagc
      181 cgaatggaag aatatttcaa aaccgctttt gagacagctg atagcataga tgaagaaatt
      241 gattttgcca gctatggaca ttatatgatt attcattccg gttcggattg gcaacatgat
      301 attatgggag atacaccatc agatttgcca tcctttttta tcaaggttgg tgatggtaaa
      361 gaagcagtag tagataatgg aacagtcctg atttctcatt cctgtaatgt tcccgaaacc
      421 atttcccagg atttttatga aaatgaagaa aacggtacta taattcacag tggctacggt
      481 gccttaaatg cagtaatagc tcatgaattc ggtcattctt taggaatggt ggatttgtat
      541 aatgtttata attcttctcc gatggttggt gtgtttgata ttatggatag tggtggttca
      601 ggcattttaa tagatgaact ggataatgga gatttggttt atgtggaagg tgctttacca
      661 actttaccgg gagctttttc ccgcaatttg atgtttcggg atagttatct ggaacgcggg
      721 ttattaaaag aattccctga ttttaacaca atgacagaat tatccttatc tgctatcagt
      781 gctatgcaaa atggaaatga tcttgttcct catacttaca aaattcccct aaatagcaag
      841 gagtatattt tagtggaaaa ccgcaatgta gaccctgatg gcgatggagg aacagctgtt
      901 tttggagctt tggatggacg agttattctc tatcctactc cttttgaaga tatttcaccc
      961 ccacctccaa cttacgaata tgactatctt ttgccttctt ttatgaaagc aaatggttca
     1021 gcaatcggag gtggtatact tgtctggcat atcaatgaag ctattttata tgatgaaggg
     1081 caaacggata gcagtggcaa ctggacaagt aattttgatg ccaataccgt taatactggt
     1141 ttttaccaca gaggtgtttc cgtaattgaa gcagatggac ttacggattt gggtagtgac
     1201 tattctatgt attggacagg aactccttac gaatattttc ataagtataa acccattttg
     1261 gataccaacg gtttatttgt aaactggagt caggaacctt ggaaaccgga attaacagct
     1321 gtcaccaaac cacctttggt agatagtttc aatgttccct gtttatatca tctgaaagaa
     1381 atcggcaatc cggcaaaaaa aatgagtttc aaggtagaaa gcggtagttt tgataccact
     1441 cagatttttg attattctac cagttccttt attatcggtc cgattataaa aacaggattc
     1501 aatgaagaca acattcctgt acttattgat aacaaaatgt tcctgctcag ttattgggat
     1561 aatgaatggc aggatattat aggcgagttt gttacaaatt ttccgcgttc agattatcct
     1621 ctgcaaaagg tagacctcaa tactgatggc tattttgaat tggtttgggc aaaacaaaat
     1681 accttggttt tagtggattg gtctttagat acgcctattt ttcaccagat ttcctttcct
     1741 gattcaatta caactacccc tttacctgta aataatacat taatagtaac tacaaaagat
     1801 tgtatttata cagtgaaaaa cttcgccatc cagaatttta tcagtttaca aggtgccaag
     1861 tgtattgccg gttatgatga taaaattata gttttaggaa cggacttttt ggctgctcta
     1921 acattagaca atctggataa gttcaatgaa tttacacttc ctgaaccttg tggcaatatt
     1981 gaaccactta tttacactaa tcccgataag accattgaga tgatttttgt aatggcaaat
     2041 agcggcaatt tgtatcggat ttatcaaaac cgtctggaat taatctttgc caatcatagc
     2101 aaggaaaaac ctggtcaact tgcctgttcc tctttagggg atatttcccc cgttctgttt
     2161 tttggtatcg gtaacaaaca atatgcctta aaagcggatg gcacaaaatt aactcatttt
     2221 ccggttattg ctccgaaagc aatttctccc tgcgaaaccc cttttgcact aaaaaacggt
     2281 tctgccgaaa ttctctttta ccctttatcg gaaactggtt atttagctat agataaaacg
     2341 ggaagaatag ttccggaaat gtgtttatcc gtaaataaag ataaaaagaa cgattattta
     2401 tattaccaac cggaacagaa aatcctgaat tggtattatc ctgatagcac aggaaggttc
     2461 tttattcaca gtaaaaagaa tattgacact aaccctattc tttttgccgg ttacagaaat
     2521 ggagcaagcg gatgtgccac ttttgccttc agagatgaaa gtatcagttc aacggaaaaa
     2581 attgcctata tttatcccaa tcctgtgcgg aagccatatt ataaacttaa tttgcaaaat
     2641 tatttcggag aaacaaaact ccatctttat gatattagtg gtactttggt aaaaaagatg
     2701 attattcccg aatccaataa taacccgagg gattttgaat taaacacttt gggactaagt
     2761 tccggggttt atattatggt tctggaaaat aacggctcca cgaaacgcct caaatttgca
     2821 gtagagaaat gattttttta gggggttagc aatgaagaaa tttatcctgc tgatttttat
     2881 tgccggtttt gccttttgcc tcttctctca ggaaaaaccc aaaaccgata ttgaccaatg
     2941 gctaaatcaa acagaagaag aacagcagaa tgcaatgcgg gattctgttg ttacaaacga
     3001 tttaattaaa ataaactaca accggaaaaa tgcccagtta gctatggcta tgtctatgct
     3061 ggttcctggt gcaggtcaat tttatgccaa taagacagtt cttacaacct atattttccc
     3121 ggttctggaa atcggactaa ttggcggact tgtttatttc aataaccaag gcaaagataa
     3181 aaccaaaaaa tacgaaaaat atgccaatgg cgaaattgta acctatatta ctccagatgg
     3241 aactaaaata acgggtttcc ggtatgatag aagtaggcaa gatgaaattc aggcaattct
     3301 gataggtgta aaccaacatg atatttatga taacggctat tttcgcttag ataagacaaa
     3361 ttctcaacat ttttatgaag acataggtaa atatcctcaa tatgtttttg gttggatgga
     3421 ttggtattat cgttttgcct gtgatgaatc aggtgtgcgt gattccattc ggtggaaatt
     3481 tgatggagaa aatgatagtc ccaatatgcg ctggactgga aattatcctt tatggggtaa
     3541 tgatactgaa acccttatcc ctattgattc ccatgaaatg agcaggatgc gtcagaccta
     3601 tattaaaatg cgtaacgacg cgaaggactc ttatagtatg gcacattttt tcactttggg
     3661 cttggctttc aatcatttag cagcaggact tgatgccatt agggttaccc gcaattttaa
     3721 ccgttattac atttctcaat cagttcctac cttaaatttc tatgccgcta tgcctttggg
     3781 aaatgtaaca ccgatgttgg ggttgaagtg gactttttga aacggaaaac ctgtcttata
     3841 attcttctgc ttttatgggg aataattccc ttattagctt tgcccaaatt tgtgtatcct
     3901 gtttcttctt taattttacc gggaacaggt gaattgcttt tagggcataa tacaaggggt
     3961 gctactttaa tgggtgtgga tttaataaat atttatgctt ttattgccac tgatcgggaa
     4021 atagagatgc agaaaaagaa ctttatgaaa tttgccgaac tttatgctgg agtgccttac
     4081 ggaatgcctg atgagcacta tcaaacagtg caaaattatc ctaatagcga ttattacaat
     4141 gatattcagg aaatgatggc tcgcaactat tatttaattt ataattatga ccctgaatcc
     4201 tttgcggaat acatttccgc caatacttat caaggcaatg aagaatggag ctggcaaagc
     4261 gaagaaaaat gggaagaata taagcatctc cgtaaacggc atcagaaaac caaaatgaat
     4321 aatcagcttg ctttggggct gatgttattg aatagaggta tcagtattat agatagttcc
     4381 attttatcta aaaagaaaca cggagaactt tatttagctc ctttacctgc aaacggtgca
     4441 atgctgaatt atcaaattga attttaggag aataactgtg aagcgttttg gcattttcct
     4501 tttaattatt ctgctttcta catcgctatt tgctcaaagc agaggaattt taactttaaa
     4561 atcttttgcc gttccaggat ggtctcaaat cagttctaac agaaattacg gctatgcaat
     4621 gcttactgta gaagcagtta taattggctc tatttattat tttcatagcg aaagcaagat
     4681 cttggaacag gaatgttatg aatatgccat taaatttgcc catttgaatc cggatggtta
     4741 tagtgatgaa ttttataaac aattagcccg ctatgaaagc agtggttttg atgccggcgg
     4801 ttataataat tgggtgcgtc aaacagctat gaatttatat cctgatgatc cggaaagtca
     4861 acagcaatat attaatgaaa atgcttatgg aaacgataaa tactggtatt gggacagttc
     4921 caaccataga agtgaatata acaaaatccg taatcggagt atggattatg ataattatgc
     4981 tcttgttgcc ggaggaatat taatcttaaa tcatttagta agtaccattg atgttttaag
     5041 gtggacagct ccaccaaaaa aagctaattt atcttttaat ttgcaagata aaaccccgat
     5101 acttcaaata aattaccact ggtaatat
//
LOCUS       DAOOZV010000091         5049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_14017_length_5049_cov_59.746296, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000091 DAOOZV010000000
VERSION     DAOOZV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14017_length_5049_cov_59.746296"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1039
                     /locus_tag="PLW08_07285"
     CDS             95..1039
                     /locus_tag="PLW08_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPL52438.1"
                     /translation="MSYLYVVIIFAILILAFISRGLIIVRQASVVIVERLGKYYRTLD
                     SGIHIIIPIFDKTRPIHWRYNKSDYRGNVVVVNKVEDRIDLRETVYDFPRQNVITSDN
                     VSININALLYFQITDPYKAVYEIGNLPEAIEKLTQTSLRNVIGELTLQETLTSRDAIN
                     AKLRDILDEATDKWGVKVNRVEMQEILPPEEIRTAMEKEMRAERDKRAKILQADGERE
                     YQIRVADGEKQARIARAEGEAQAKILVADSEKKAILLIAEAVKDSKTDPAQFQIALRY
                     VEAFKELVKQGDKTVVLPYESSALLGSVKTLGDIFQKT"
     gene            1248..1727
                     /locus_tag="PLW08_07290"
     CDS             1248..1727
                     /locus_tag="PLW08_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPL52439.1"
                     /translation="MRYYSIFALVLLLLFCACNPKTAEKTETPFDPSALITQIDTIMK
                     ELLTDAENLKTGVLEKYLSNDPTDMYYLGAKVYTRKDLISELKQEYASIANQKLEILQ
                     QKTYILGNEAAVWIANIRGIATNKDKKTIETIHTETWLWQKVNNEWKITHFNKSWQM"
     gene            1846..3045
                     /locus_tag="PLW08_07295"
     CDS             1846..3045
                     /locus_tag="PLW08_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPL52440.1"
                     /translation="MIQDMYEDREFYASELKELLRETLKHSIAGEFSAHSEDPYFYTQ
                     KEYPLQTEIEGLALYPEFIPEVLLNWANLDTSKERKKEDILFLDLETTGLRSNGIFAF
                     MIGLGYYVNEQFIVEQIFLPDPEAEVNSFDRLLELVNEKSLLITFNGKTFDIPVLESR
                     LLYHQIWLNLRAMEHLDLLHLARRLWKNKLPSCALETIEFYILGQIRDKELDIEGGEI
                     PQTYFSFLLNGNPELMQRVFLHNHTDILHTAALFTLICNSISYPPQGGMDSRIDYHSL
                     AMLYQSQNRIDIAKNILEDMLSAGITDKDVLRDLGFIYKKEKDMESALKCFNIASSLN
                     CPVSMHETCIILEKYYKRYAEALVKAEKLKSYLISRPIVNDQKVAAIDKRIARLKLKL
                     NKVNEVI"
     gene            3046..4092
                     /locus_tag="PLW08_07300"
     CDS             3046..4092
                     /locus_tag="PLW08_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPL52441.1"
                     /translation="MDSVSFGSDNHSGIHPQVLAAIQKANEGFCPAYGDDPLTVSVLN
                     EIKELFGGNCDAWFVITGTGANILCLQSLMHSYNAIFCPQSAHINTDECGAVQKFTQG
                     RLLPIETADGKLTPELIAPHLLGNRDQHHSQAKIISLSQSTEYGTLYTLQELQKLADF
                     AHQNNMFLHIDGARLANAAAALNCSLKEMTKDISADIVSFGGTKNGLLCGEAIISFRP
                     ELTSAMRFYRKQASQLLSKMRFISAQYQAYLEDDLYLKNARTANAMAKLLAERLREIP
                     ELIVTQEVAVNSVFVILPRQIIEPLQQKYHFYTWNEALNEVRLMCSFNTTETHIESFI
                     QDLKALLVIKNPHW"
     gene            4286..4894
                     /gene="wrbA"
                     /locus_tag="PLW08_07305"
     CDS             4286..4894
                     /gene="wrbA"
                     /locus_tag="PLW08_07305"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HPL52442.1"
                     /translation="MKVLVLFYSAYGHIYKMAEAVAEGARKVEGMEVEIKQVPETLSS
                     EILDKIGATEAKKAFAHIPIAEIEDLTTADAIIFGFPTRFGSMPSQMKTFIDGTGSLW
                     AKGALIGKIGSVFTSTSAQHGGQESTILGFYPILLHHGMLITGLPYSFQGQGTMDEIS
                     GGSPYGASTVVGDGSRMPSENELEGARYQGWYVASLVAKMHK"
BASE COUNT         1633 a          904 c         1012 g         1500 t
ORIGIN      
        1 taagaactat aaaaccaccc tctcatcctt cacaattttt cttgccaaaa aaacacagcc
       61 gggaaataag gaaaaaaatg aaagggaggt aacaatgtct tacctatatg tggtaatcat
      121 ttttgcaatc ttaattcttg cttttatttc tcgcggactg ataattgtcc gtcaagcaag
      181 tgtagtaatt gtagaacgct taggaaaata ttaccggact ttggattccg gaattcatat
      241 tatcattccc atttttgata aaacgcgtcc tattcattgg cgttataaca aaagtgacta
      301 tcgcggaaat gtagttgtgg tcaataaagt ggaagataga attgacctta gggaaactgt
      361 gtatgatttt ccccggcaga atgttatcac cagtgataat gtttccatca atattaatgc
      421 tttgctttat ttccagatta cagaccccta taaggcagtg tatgaaatcg ggaatttacc
      481 cgaagcgatt gaaaaactca ctcagacctc tctcagaaat gttattgggg aattaacctt
      541 gcaggagacc ctcacttccc gggatgctat taatgccaaa ttgcgcgata ttttagacga
      601 agcaaccgat aaatggggcg tgaaagtaaa ccgggtggaa atgcaagaaa ttttgcctcc
      661 cgaagaaatt agaacagcta tggaaaaaga aatgcgcgcc gaacgcgata aacgagccaa
      721 aattttgcaa gcagatggag aacgcgaata tcaaatccga gttgcagacg gtgaaaaaca
      781 agctcgtata gcccgtgcag aaggtgaagc acaagccaaa atattggttg ccgatagcga
      841 aaagaaagca attttactga ttgcagaagc cgttaaagat agcaaaacag accctgctca
      901 atttcaaatt gcgcttagat atgtggaagc atttaaagag cttgtaaaac aaggagataa
      961 gacagttgtt ctgccttatg aatcatctgc ccttttaggc agtgtaaaaa cattaggaga
     1021 cattttccag aagacataag agatagtata cagttgttta gctcaataat gcagtggaag
     1081 taattaatag acatattgag taattgggtt gagaggattg agaaggttaa aattattttc
     1141 tccagacctc tgtgcctaat tcaaaccttg acaaaataat tcccattcag ctaaggtatt
     1201 tttggaagca gaacgaaaac aaccaaataa ccgcttcagg aggtatgatg agatactatt
     1261 ctattttcgc cttggtgtta ctactacttt tttgtgcttg caatccgaaa acagcagaaa
     1321 agacagaaac accttttgat ccatcagctc ttattacaca aattgatact ataatgaagg
     1381 aattattaac agacgctgaa aatctgaaaa ccggcgttct ggaaaaatac ctgagcaatg
     1441 atcccacaga tatgtattat ttgggtgcga aggtttatac caggaaggat ttaatcagcg
     1501 aattaaaaca ggaatatgca tccatagcta atcaaaaatt ggaaatccta caacagaaga
     1561 cctatatttt aggaaatgag gcagccgtct ggattgccaa cattcgggga attgctacaa
     1621 acaaagataa aaagaccatt gaaacaattc acactgaaac ctggttgtgg cagaaagtta
     1681 ataatgaatg gaaaataacc catttcaaca aatcctggca gatgtagcaa tcttcttttt
     1741 gtattattta tatcagttct tgaaggaaaa gtcctttgtt attccggcta aagcaaaaat
     1801 aaaaaagtta tatgcattta ccaacagaaa ttcagaagat aagttatgat tcaagatatg
     1861 tatgaggata gagagtttta cgcttcagaa ctgaaagaac tgttaagaga aacattaaaa
     1921 cattctattg caggtgaatt ttccgctcat tccgaagacc cttattttta cacccaaaaa
     1981 gaatatcccc tgcagacaga aattgaaggt ttagctcttt atcctgaatt tattccggag
     2041 gttttgctca attgggcaaa cctggataca agcaaagaac ggaagaaaga agacatcctt
     2101 tttctggatt tggaaacaac cggtttacgc agtaatggca tatttgcttt tatgatcggt
     2161 ttgggttatt atgtaaatga gcagtttatt gtggagcaaa tttttcttcc cgatcccgaa
     2221 gcggaggtaa attcttttga tcgcctgctg gaactggtaa acgaaaagtc cctactgatt
     2281 acttttaacg gcaagacctt tgatattcct gttttggaat cccgtttgct gtatcatcaa
     2341 atctggctga atttaagagc aatggaacat ttggatttac ttcatcttgc ccgtcgttta
     2401 tggaaaaaca agcttccttc ctgtgctttg gagaccatag agttttatat tctgggtcaa
     2461 attagagaca aggaactgga tattgaaggt ggcgaaattc cgcagaccta tttcagtttt
     2521 ttgttaaatg gcaatccaga gcttatgcaa agggtttttc tgcataatca tacggacatt
     2581 ttgcatacgg cagctctttt tacacttatt tgtaacagta tcagttatcc tcctcaaggg
     2641 ggaatggaca gtagaattga ttatcattct ttggcaatgt tgtatcaaag tcagaaccgc
     2701 attgatatag ctaaaaacat tttagaggat atgctttcag ctggaataac agataaagat
     2761 gttctgagag acctgggctt tatttataaa aaggaaaagg atatggaatc cgctttaaag
     2821 tgttttaata ttgcctcttc cttaaattgt cccgtttcaa tgcacgagac ctgtatcatt
     2881 ctggaaaaat attataaacg ctatgcggaa gcccttgtta aagccgaaaa attaaagtcc
     2941 tatttaatta gccgtccaat cgttaatgac caaaaagtag ctgctataga taaacgcatt
     3001 gcccggctta agcttaagct aaataaggta aatgaggtaa tataaatgga ttcagttagt
     3061 tttggttcgg ataatcacag cggaatacat ccccaagttt tagcagccat ccaaaaagct
     3121 aatgagggat tttgtccggc ttatggagat gatcctttaa cagtaagtgt tttaaacgaa
     3181 attaaagaac ttttcggagg aaattgtgat gcctggttcg taattactgg aactggagct
     3241 aatatattat gtttgcagtc acttatgcac tcctataatg ccattttctg tccccaaagt
     3301 gctcatatca acacagacga atgtggtgct gtccaaaaat ttacccaggg acgattactg
     3361 cccatagaaa cagcggatgg aaaacttact ccggaactca ttgcacctca tttattggga
     3421 aatcgtgatc aacatcattc ccaggcaaaa ataatttccc tctctcaaag cacggaatat
     3481 ggaactttat atactttgca ggaattgcag aagttagccg attttgctca tcaaaacaat
     3541 atgtttttac atattgatgg agcacgtttg gcaaatgcgg cggcagcttt gaattgttcc
     3601 ctgaaagaaa tgactaaaga tatttctgcc gatattgtca gttttggagg caccaaaaat
     3661 ggtctacttt gtggagaggc aattatcagt tttcgtcccg aattaacttc cgctatgcgt
     3721 ttttaccgta agcaggcaag tcagctttta agtaaaatgc gttttatttc tgctcagtat
     3781 caggcatatt tggaagatga tttatatttg aagaatgcca gaactgcaaa tgcaatggca
     3841 aaactattgg cagagcgatt aagagaaata ccggaattaa ttgttaccca agaagttgct
     3901 gttaattctg tgtttgtaat tttgccccgg cagataattg aacccctaca acaaaaatat
     3961 catttttaca cttggaatga ggcattaaac gaagttcgtt tgatgtgcag ttttaataca
     4021 accgaaacac atattgagag ttttattcaa gacctcaaag ctctattagt catcaagaat
     4081 cctcactggt aaacaaaata gagaatactg caaaagttat ttcagaggtg acaactccca
     4141 acttacagcg taaaaaaacc ctcataaaat aaaatctgtg ttatctgtgc tatctgtgag
     4201 agcaaaaaat tatcttccgc tcttgccaag ttggattttc cagttaatat atccataaag
     4261 cttaataata aggagtttac ctaaaatgaa agtattagtc ctcttttact ctgcctacgg
     4321 tcacatttac aaaatggcag aagcagttgc ggaaggtgca agaaaagtag aaggaatgga
     4381 agtagaaata aagcaggttc cggaaacgct aagttctgaa attctggata aaataggagc
     4441 aacggaagca aaaaaggcat tcgctcacat tcctattgct gaaattgaag atttaaccac
     4501 agcggatgct ataattttcg gttttcccac gcgttttggc agtatgccct ctcaaatgaa
     4561 aacctttatt gacggaaccg gttctttatg ggcaaaaggt gctttaatag gtaaaatcgg
     4621 cagtgttttt acctctactt cggctcagca tgggggtcag gaatccacta ttttgggttt
     4681 ttatcctatt cttttgcatc acggtatgtt gataacaggt ctaccctatt cttttcaagg
     4741 tcagggaact atggatgaaa tttccggtgg ctctccttat ggagcttcta cagttgttgg
     4801 agatggcagt agaatgcctt cggaaaacga attggaaggt gcacggtatc agggttggta
     4861 tgtggcttcc ttggtagcaa aaatgcataa ataggatatt tataactatt tcctaatgcg
     4921 aggtatctgt tccagtggca gatacctcgc ttattgtgtt gcctatatca agaccgtaat
     4981 aacagttttt ttgctcctgc ctgttcctgt ttatgccaat ttattgtttg attatcttaa
     5041 ccgctatat
//
LOCUS       DAOOZV010000092         5040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_14048_length_5040_cov_66.451555, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000092 DAOOZV010000000
VERSION     DAOOZV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14048_length_5040_cov_66.451555"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1507)
                     /locus_tag="PLW08_07310"
     CDS             complement(50..1507)
                     /locus_tag="PLW08_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPL52443.1"
                     /translation="MNKNKSKVLTIIFLAASVFVTAVPVTILHTNDTHGAYLPQNSRF
                     GKIGGYAVLEYYLNAEREKTSAALYLDAGDQQTGSIFASLTYDNAIGGAVIKVFNYLN
                     LDATTYGNHEFDYSQDNTIKLRELANYPFLSTNILDKNNQPFGGIPYKIFSLDSLQIG
                     VMGLTLTELPEKVKRENIAGLTILPYKEAIDKYIEEVDKQSDLIILLTHNGFEADSLL
                     ATVLDNRVDLIIGGHSHTVISEPCQVNGIYIASTGSYLTYLGAINLEVQNDRIIAYAS
                     KLIPLLEPENLPITPLNQFVQTMADSTEKETGKVIAHIPVDWIPDKFKETAVSRWAAE
                     ALKQEYYESCKPDLAIINNGGIRKVIPAGPVTLKDMYELFPFNNYIVLFSCYGRDLLT
                     MEALNKEIAKNKPYDIVQTSSTEWKKKKRLFGLLKPKEVYYINGKPVDPNKIYRVVSF
                     DYVLGQWDKYLGFKPFDVQETGELFTEVMIRQVKK"
     gene            complement(1575..2303)
                     /gene="kdsB"
                     /locus_tag="PLW08_07315"
     CDS             complement(1575..2303)
                     /gene="kdsB"
                     /locus_tag="PLW08_07315"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPL52444.1"
                     /translation="MNIIALIPARYNSTRFPGKPLALLNGKPIIQYVYERVANSGLFN
                     TVCVATDNISILETVKSFGGKAVLTKESHPSGSDRIAEALQYFPQAELVFNVQGDEPL
                     IEEEPLQKLISAFEDRKVQMASLMTVIEDREMIYNPNIVKVVTDTQNYALYFSRSVIP
                     FQRDSQIDLKYYRHIGVYAYSPECLHRFVYLSPSILERTEKLEQLRALENGIPIKMVE
                     TSYQGIGIDTPQDLKTIEKQLSVT"
     gene            complement(2300..4147)
                     /gene="mnmG"
                     /locus_tag="PLW08_07320"
     CDS             complement(2300..4147)
                     /gene="mnmG"
                     /locus_tag="PLW08_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPL52445.1"
                     /translation="MHTDNKFDLIVVGAGHSGIEASLAAAKRGLKVALFTIKIEAIGR
                     MSCNPSIGGPAKGHLAREIDALGGELAKSADLSGIHFRMLNRSKGPAVWAPRSQNDRQ
                     KYHLLMREAVEKQENIHLIEATIEEIIVENGCVKGVISQIGHCYYAPKVILATGTFLQ
                     GKIHIGKISYAGGRSGEPSVEYLSRSLSAWGLKVRRFKTGTPPRVDLRSVNYNKLEEQ
                     KGDENPQGFSFYRDIELKNLVSCYITHTTEETHQIIRANLTESALYSGIIKGIGPRYC
                     PSIEDKIVKFPQRASHHIFIEPEGLNTFEGYVNGISTSLPAEIQEKIVHSIPGLEEAR
                     ILRYAYAIEYDFIDPEEIDSSLQCKKIKGLYLAGQINGTSGYEEAAAQGLLAGINASL
                     ALENKEPLILKRSEAYIGVLIDDLVTRGTNEPYRMFTSRAEYRLLLRQDNADERLMPV
                     GYKLGLVEESRWQRFLQMQEILQRELKYLKQHNCLPNGEIKEPIRFAQLLKRPEISFN
                     DLQNYGYKIPADVNSDIQNRLELEIKYSGYLNRMEEEIQKFQNAETIAIPEEMDYFSI
                     PSLAYEAREKLSKIRPKNIGQAMRIPGINYSDSVALMIWLRKNAKKVAK"
     gene            complement(4149..4760)
                     /gene="yihA"
                     /locus_tag="PLW08_07325"
     CDS             complement(4149..4760)
                     /gene="yihA"
                     /locus_tag="PLW08_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPL52446.1"
                     /translation="MIRFVESFFVKSAIEPSDYPASAYPEFAFAGRSNVGKSTLINLL
                     TNHKGLAKTSSTPGKTRLLNFFLIRYKIDDNDSSGFLQFTDLPGYGFAEVSQAEQEKW
                     RRMINKYFEQRKQLRSLIVIVDIRHPADSKDIMLLEMLRLRGIDYCIVATKADKIPKT
                     KVAKTVKDLAKGLGLTEEPIFPVSALNNTGLEPLYNWFQNKLS"
     gene            complement(4744..>5040)
                     /locus_tag="PLW08_07330"
     CDS             complement(4744..>5040)
                     /locus_tag="PLW08_07330"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPL52447.1"
                     /translation="HNWKVRVCVKTKEQDEIGMAMDFGVLKTILSNILNNLDHSYLNE
                     IVPFTERNPTSENLAKYIYECMEKELQDKPVAISEVEVYESEKSSVIYKNDQIC"
BASE COUNT         1439 a         1051 c          908 g         1642 t
ORIGIN      
        1 caattctcaa ttctaatagg ttttccttgt taatttcatt cttcatttat cattttttta
       61 cttgtctaat catcacttcg gtaaagagtt cacctgtttc ctgtacatca aagggcttaa
      121 aacccaaata tttatcccac tgacccaaaa catagtcaaa gctaacaacg cggtatattt
      181 tatttggatc aaccggtttt ccattgatat aatagacctc tttgggtttc agcaaaccga
      241 atagacgctt tttctttttc cattccgttg aacttgtttg cacaatatcg tagggtttgt
      301 ttttagcgat ttctttatta agtgcttcca tagtcaataa atctcttcca taacaggaaa
      361 acaggacaat gtaattattg aagggaaata gttcatacat atctttgagg gtaacaggtc
      421 ctgccggaat aacttttcta atacctccgt tattgataat agccaaatcg ggtttacagg
      481 attcataata ttcctgtttt aatgcttcag cagcccatcg ggaaactgct gtttccttaa
      541 atttatcggg aatccagtcc actggaatat gggcaataac ttttcccgtt tctttttctg
      601 tgctgtctgc cattgtttgc acaaattgat ttaggggtgt tataggtaaa ttttccggtt
      661 ccagcaaggg aataagtttg cttgcataag ctataatgcg gtcgttttgc acttctaaat
      721 tgatggcacc caaataggtt agataacttc cggtggaggc aatgtaaatt ccattcactt
      781 gacagggctc agaaataacg gtatgggaat gacctccaat aattaaatcc acccggttat
      841 ccaaaacggt tgccaataag gaatccgctt caaagccatt atgggtaagc agtattatca
      901 gatcgctctg tttgtctact tcttcaatat acttatctat tgcctcttta taaggcagaa
      961 tggttaatcc ggcaatattt tcccttttca ccttttccgg cagttcggtt aaagttaatc
     1021 ccattacacc aatttgcaag gaatccaagg aaaatatttt atagggaatt ccgccaaagg
     1081 gttgattatt cttatccaga atattagtgc tgagaaaagg ataatttgcc agttccctaa
     1141 gtttgattgt gttgtcttgc gaataatcaa actcgtgatt accgtaagtt gtggcatcca
     1201 gattgagata attgaagacc tttataacag cacctccaat ggcattatca taagttaagg
     1261 aggcaaaaat gctgcccgtt tgttgatctc cggcatccag ataaagcgct gctgatgtct
     1321 tttccctttc tgcattcaaa taatattcca gaacagcata accgccaatt ttgccaaacc
     1381 ttgaattttg aggtagatag gcaccatggg tgtcgttagt atgcaaaata gttacaggaa
     1441 cagcagtgac aaaaacactg gcagctaaaa agatgatagt taggactttg cttttgttct
     1501 tattcataat tcttctcttc tctggattcc tgcttgcgca ggaatgacaa aaaactttca
     1561 ttcaaaaact aatatcaagt aacagaaagc tgtttctcta ttgttttcag gtcttgtggt
     1621 gtatcaattc cgatgccttg ataactggtt tctaccattt taataggaat tccgttttcc
     1681 agagctctta gctgttccaa tttttctgtc ctctctaata tagagggtga caaatataca
     1741 aatcggtgca aacattccgg agaataggca taaacaccaa tgtgacgata atatttaagg
     1801 tctatttgac tgtctcgctg aaaaggaata acggaacggg aaaaatacaa agcataattt
     1861 tgcgtatcag ttacaacttt tacaatgttt ggattgtata tcatttcccg gtcttcaatc
     1921 acggtcatta aacttgccat ctgcactttt ctatcttcaa aagcggaaat aagcttttgt
     1981 aagggttctt cttcaataag cggttcatct ccctgaacat taaaaactaa ttctgcctgc
     2041 ggaaaatatt gtagcgcttc agctatgcgg tctgaaccgc tgggatgact ttcttttgtt
     2101 aaaaccgctt tgcctccgaa tgacttaact gtttctaaaa tggagatatt atcagttgct
     2161 acacaaacag tattaaataa accggaattt gcaacgcgtt catatacata ctggataatc
     2221 ggttttccat tcagcaatgc caagggtttt ccgggaaaac gcgttgaatt ataacgagct
     2281 ggaattaagg caataatatt catttggcta cctttttggc atttttgcgc agccaaatca
     2341 ttagggcaac gctatcacta taatttattc ccggaattcg cattgcctgc cctatgtttt
     2401 taggacggat tttactaagt ttttcgcgtg cttcataagc cagagaggga atgctaaaat
     2461 agtccatctc ttcaggaatg gcgattgttt cggcattttg gaacttttga atttcttctt
     2521 ccattcggtt taaatagcca gaatatttaa tttccagttc aaggcgattt tgaatatcac
     2581 tattaacatc cgccggaatt ttgtaaccgt aattttggag gtcattaaaa gaaatttcgg
     2641 gtcttttcaa taattgagca aaacggatgg gctcttttat ttctccattt ggcaggcagt
     2701 tatgctgttt caggtatttc agctcccgct gtaaaatttc ttgcatctgc aaaaaccttt
     2761 gccaacgact ttcttctacc aatcccaatt tgtaaccaac cggcattaaa cgctcatcgg
     2821 cattatcctg acgtaaaagt aaacggtatt ccgcacgcga agtaaacatt ctatagggct
     2881 catttgttcc ccgggttaca agatcgtcaa ttagaactcc gatgtatgcc tcactgcgtt
     2941 ttagaataag gggttcttta ttttccaaag ccagagaagc atttataccc gctaaaagac
     3001 cttgtgctgc tgcctcttca taaccggaag tgccatttat ctgtcctgcc aggtataagc
     3061 cctttatttt tttgcactgc agagaactat ctatttcttc cggatcaata aaatcatact
     3121 ctatagcata ggcatagcgt aaaatacggg cctcctccaa acccggaata ctatgcacaa
     3181 ttttttcctg aatttctgcc ggaagcgaag tagaaatgcc gtttacataa ccttcaaagg
     3241 tattcagccc ctccggttca ataaaaatgt gatgacttgc cctttgggga aatttcacta
     3301 ttttatcctc aatagaagga cagtaacgcg gtccgatacc tttaattatt ccggaataaa
     3361 gagcggattc agttaaattg gctctaataa tttgatgcgt ttcttccgta gtgtgtgtga
     3421 tataacaaga aaccagattc ttaagttcaa tatcccggta aaaagaaaag ccctgaggat
     3481 tttcatcccc tttttgttct tccagtttat tataatttac agaacgcaag tccactctgg
     3541 gtggagttcc cgttttaaac ctgcgaactt ttaatcccca agcagataaa gaacgggaaa
     3601 gatattctac agatggctct ccactgcgtc ctccagcata acttattttg ccaatgtgga
     3661 tttttccctg caaaaaagtg ccggttgcta aaataacttt aggagcataa tagcaatgtc
     3721 cgatttggct tattacccct ttaacacaac cattttctac aattatttct tctatggttg
     3781 cttcaattaa atgaatattt tcctgttttt ctactgcttc tcgcatcagc agatggtatt
     3841 tttgacgatc attttgagaa cgcggagccc aaacagccgg acctttactt ctattcagca
     3901 tccgaaaatg aattccggaa aggtcggcac tttttgccag ttcaccacct aaggcatcta
     3961 tttctcttgc cagatgacct ttggcaggac ctccgatgga tggattacag ctcattctgc
     4021 ctatagcttc aattttaatg gtaaagagag caactttcaa ccctcttttt gctgctgcca
     4081 gggatgcctc aattccggaa tgacctgctc ccacaacgat gagatcaaat ttgttatccg
     4141 tatgcatttt agcttaattt attttgaaac cagttataaa gtggttccag acctgtatta
     4201 ttaagtgccg aaacaggaaa aatcggctct tctgtcaagc ccaaaccctt tgccaggtct
     4261 ttcaccgtct tggctacttt ggttttggga attttatctg ccttggtagc tacgatacaa
     4321 taatcaatac ctcttaagcg taacatttcc agtagcataa tatctttgct gtctgccgga
     4381 tgacgaatat ctacaataac aattagagaa cgcaattgct tgcgctgttc aaaatattta
     4441 ttgatcatcc gacgccattt ttcctgttct gcctggctaa cttcggcaaa gccgtaacct
     4501 ggtaaatcgg taaattgcaa aaagccggaa ctatcattat cgtctatctt ataacgaatt
     4561 aaaaagaaat tcaataagcg tgtctttcca ggagtggatg atgttttagc aagcccttta
     4621 tgattggtta acaaattgat aagggtagat ttgccgacat tgcttctacc ggcaaaagca
     4681 aattcgggat aagcagaagc cggataatca ctcggttcaa tagcactttt aacaaaaaag
     4741 gactcaacaa atctgatcat ttttataaat cacactgctt ttttcggatt cgtaaacctc
     4801 tacttcagag atagctacgg gcttatcctg aagctctttt tccatacatt cataaatgta
     4861 tttagccaga ttttcggaag tgggatttct ttccgtaaac ggaacaatct cattcaaata
     4921 agaatgatcc aggttattta gtatgttaga cagaatagtt ttgagaacac cgaaatccat
     4981 tgccatcccg atttcatcct gctcttttgt cttcacacaa accctcacct tccagttgtg
//
LOCUS       DAOOZV010000093         4700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_15178_length_4700_cov_74.121851, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000093 DAOOZV010000000
VERSION     DAOOZV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15178_length_4700_cov_74.121851"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..928
                     /locus_tag="PLW08_07335"
     CDS             245..928
                     /locus_tag="PLW08_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL52448.1"
                     /translation="MDIIKTENIVKDYILGKIKVRALNGIDLQIQKGEFVAIMGPSGS
                     GKSTLMHILGCLDSPTDGTYYLDDVLVSKMPKASLAAVRNRKIGFVFQSFNLLPHLNI
                     LKNVELPLMYGSMSKRKRTAKAKEVLQNVGLGDRLKHKPGELSGGQRQRVAIARAIVN
                     EPSILLADEPTGNLDSQSGGDILEIFTELHSQGNTVIIVTHDQAIASRAERIIKIKDG
                     KIVDGNSNS"
     gene            909..2144
                     /locus_tag="PLW08_07340"
     CDS             909..2144
                     /locus_tag="PLW08_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL52449.1"
                     /translation="MAIPIAESLKLGFADIMMRKVRSIVTVIGIILGVMCIMVVLAIV
                     NGMNKSTMSWMEERGGLNKIEVEQNWFYDFSKGGDPSFSLKEIRYLQSLIPEAEAFNP
                     TVQEWEAVINKGDIRYSTSLRGVMPDFVKVEQWDIAKGRFIKDLDIDQHSNVIVLGST
                     VAKEFFGNNDPLGQIISVGNQQFIVVGVMAERFMPAQGGQMGMGENALEYLNRRCFIP
                     ISTMISKVNPGSKISSIDIRAKSPEEAKELRRKVENIVLNLKNGKHLFQVTSAKEQLD
                     TMQANAKIFEAIFVLIAVISLLVGGIVIMNIMLASIRERTREIGVRIAVGARRRDIFI
                     QFLVQTVLITALGGILGILLGFAILDKVGSYLQIKVLASVQMIWVALLVSIGVGLIFG
                     VGPAVRAARLDPVIALREE"
     gene            2144..3223
                     /locus_tag="PLW08_07345"
     CDS             2144..3223
                     /locus_tag="PLW08_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HPL52450.1"
                     /translation="MSKKKQNKGVFQKLLLWLYSYIKIVISDLKKIYQSITVPQQRKK
                     VFGNIWTFIKTFYQRFMGEGILKESASLTYITILGFVPFINFLVLIAPDLPFLNLGDK
                     LRELVVQNFIPSSANAIINFLDKLIMPKVGFNIFNFVILIVSSYSLFSVIRGTFDRIL
                     SMQLKSSQDTITQLVKFFGTIVFGLLIIVLLFSSSSIPIISRLLKSPVLQWIMVIVPF
                     ILQFLAIMFLYMLLPSVRINRASLFRGAFWTTVIWVIGKSVFDFYIYNLTNYERLYGV
                     MAVLPIFLLWIYINWAIILGGMVLVSEMENKKNGGFLTKEPHNAVRITMELFTNQKLN
                     QRLEGILRKEELKKLTDTLNEEDDK"
     gene            3220..>4700
                     /gene="purH"
                     /locus_tag="PLW08_07350"
     CDS             3220..>4700
                     /gene="purH"
                     /locus_tag="PLW08_07350"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPL52451.1"
                     /translation="MNKYALISVSDKTGIEYLAVELEKMGYTILSTSHTADYLKQFCQ
                     KVVLVSDLTKFPEILEGRVKTLHPVIYAGILADRNNPAHAKTLSELKIEHIDVIAVNL
                     YPFASVCRKENATEQEIIENIDIGGPALIRAGAKNYKSVTVLVDPVDYAPALELLKQN
                     SILPEKFSSYLAQKAFAKTRDYDAEIAAYFASQGKEESITNELPAQLEFSISLKRKLR
                     YGENPHQNGGFYTNVNPGWELIHGKELSFNNIMDMDSAFRAIRLFAKPTAIIIKHCNP
                     CGIGSGETLAEAYRKAYETDTEAPFGGIVIVNRPLDLETATLINNIFTEIIIAPAFEP
                     NVLEFLKKKKNRRLIHYEFSLLEKPLNPVEIKTLTCGYLAQDWDLVNESTENWKIVTN
                     KQPAPEEWEAIIYAWKAVSILKSNAIAIAKKDRVLGLGCGQTSRIDAVQLALWKAKKF
                     GHDLTDSVCASDGFFPFRDCIDTLAKNGISAIIQPGGSKNDEE"
BASE COUNT         1459 a          832 c          979 g         1430 t
ORIGIN      
        1 aaactcgcga tagacaaaaa aaggcaaaat tattataata acatccgcaa aatctgcgtg
       61 ctaactcgct atgagtcctt actgttaaat aaaggggtat aaggtttata acttttaaaa
      121 gcaatgacaa ctcctaactt gcatagaaat ctgtgaaatc cgtgttatct gtgagagatt
      181 cactttttca ttgacacaat agcagataat aagtgaaagg attataaagg aaaaataggt
      241 aaatatggat attattaaaa ccgaaaacat agtaaaggac tacattttag gcaagataaa
      301 agtccgtgcc ttaaatggaa ttgacctgca aattcagaaa ggtgaatttg ttgccataat
      361 gggtccctcc ggctcgggga aaagcacttt aatgcatatt cttggatgtt tggatagtcc
      421 aacagatgga acttattatc tggatgatgt tttggttagc aaaatgccca aagcatcttt
      481 agctgcggtc agaaatcgga aaatcggctt tgtgtttcag tcctttaatc tgctccctca
      541 tttgaacatc ttgaaaaatg tggaactccc tttaatgtat ggaagtatga gtaaaagaaa
      601 acgcactgcc aaagcaaaag aggttttaca aaatgtgggt ctgggagatc gtttaaaaca
      661 taagcccggt gaactttccg gaggtcaaag acaacgcgtt gccatagccc gggcaattgt
      721 aaatgaacct tccattctgc tggcagatga accaacggga aatctggatt ctcaatccgg
      781 aggcgatatt ttggaaattt ttacggagtt acacagtcaa ggcaataccg ttattattgt
      841 aactcacgac caggcaattg cttcccgagc agaaagaata atcaaaataa aagacgggaa
      901 aatagtagat ggcaattcca atagctgaat ccctaaaact tggtttcgct gatattatga
      961 tgcgtaaagt tcgcagcata gtaaccgtga ttggcataat tttaggtgtg atgtgcataa
     1021 tggttgttct ggcaattgtg aatggaatga acaaaagcac aatgagttgg atggaagaac
     1081 gcggtggatt gaacaagata gaagttgaac aaaactggtt ttacgatttt tccaaaggtg
     1141 gcgatcccag tttttcttta aaggaaattc gttatttgca atcccttatt ccggaagcgg
     1201 aagcatttaa tcctacagtt caggaatggg aagcggtaat aaataagggt gatattcgtt
     1261 acagcacatc tttaagaggt gtaatgcccg attttgtaaa agtggaacaa tgggacatag
     1321 ctaaaggcag atttattaaa gacctggata tagaccaaca tagcaatgta atagttctcg
     1381 gctcaaccgt ggcaaaagag ttttttggca acaacgatcc tcttggacaa attatttccg
     1441 taggcaatca acaatttatc gttgtcggtg taatggctga aagatttatg ccagcacagg
     1501 gtggacaaat gggaatgggt gaaaatgctt tggaatattt aaaccgccgt tgttttattc
     1561 ctatttccac aatgatcagt aaagttaacc ccggcagtaa aataagctcc atagatatta
     1621 gagcaaaaag tcctgaagag gcaaaagaac tgcgccgtaa agtggaaaac atagtgctga
     1681 acctgaaaaa cggaaaacat ttgtttcagg ttacttccgc taaagaacaa ctggatacaa
     1741 tgcaggcaaa tgctaaaata tttgaagcca tatttgtttt aatagccgta atttctctgc
     1801 tggttggggg aattgtaatt atgaacatta tgcttgcttc catccgggaa cgcactcgcg
     1861 aaataggagt tcgcattgcg gttggagcca gaagaagaga tatttttatt cagtttttgg
     1921 tgcaaacggt gcttattacc gctttaggtg gaattttggg aattctttta ggatttgcta
     1981 ttttggataa agtgggttct tatttacaaa taaaagtgct tgcctcggtg caaatgattt
     2041 gggttgctct tttagtttct attggagtgg gacttatttt tggggttggt cctgctgtca
     2101 gagctgcccg tcttgatccc gtaattgctt taagggagga ataatgagta aaaagaaaca
     2161 aaataaaggt gtttttcaga agctccttct ttggctatat tcttacatca aaattgttat
     2221 ttcggacctc aagaaaattt accagtccat aactgtccct caacaacgga aaaaggtctt
     2281 tggcaatata tggactttta tcaaaacatt ttaccagcgt tttatgggcg aaggtatttt
     2341 gaaggaatca gccagtttaa cttacataac tattctcggt tttgtccctt ttatcaattt
     2401 tctggtttta attgccccgg atttaccatt tctgaatttg ggtgataaac tgagagaatt
     2461 agtggttcaa aacttcattc cgagttcagc aaatgctatt atcaactttt tggacaagct
     2521 gattatgccc aaagttggct tcaacatttt caattttgtt atcctgattg ttagttccta
     2581 ttccttgttt tcagttatca gagggacttt tgaccgtatc ctgagtatgc agctgaagtc
     2641 ctctcaggat actattactc aattagtgaa atttttcggc acgattgttt tcgggttgct
     2701 gattatcgtg ttgctatttt ccagttcatc tattccaatt atctcccgtt tattaaagtc
     2761 ccctgttttg cagtggatta tggtaatagt tccttttatt ttgcaatttt tggctataat
     2821 gtttctttat atgctgttgc cttcggtaag aattaaccgt gcctctttgt ttagaggtgc
     2881 tttttggact acggttatct gggttattgg taagtccgta tttgattttt atatatataa
     2941 cctaactaat tacgaacgac tttacggagt gatggctgtt ctaccgatat ttttgctgtg
     3001 gatttatata aattgggcta ttattttagg aggaatggtt ttagtaagtg aaatggaaaa
     3061 caaaaaaaac ggtggctttt taaccaaaga accccataat gctgtgcgca taacaatgga
     3121 actttttact aatcaaaaac ttaatcaacg cctggaagga attttgcgta aagaagaact
     3181 gaagaaattg acagataccc tgaacgagga agatgataaa tgaataaata tgcactaatt
     3241 agtgtttccg ataaaaccgg cattgaatat ttagcggtgg aactggaaaa aatgggctat
     3301 accattcttt ccacttcgca cacagccgat tacttaaaac aattctgcca gaaagtagtg
     3361 ctggtttcgg atttaaccaa atttccggaa attctggaag gcagagttaa aacactccat
     3421 cccgttattt atgccggaat tttggcagat agaaataatc ctgctcacgc caaaacactc
     3481 tctgaactaa aaattgagca catagatgta atagcggtaa atttgtatcc ttttgcctct
     3541 gtttgccgga aagaaaatgc caccgaacag gaaattatag aaaatataga tattggaggt
     3601 cccgctttaa tccgtgcagg agcaaaaaac tataaaagcg ttaccgtttt ggttgatcct
     3661 gtggactatg ctcctgcgtt ggagctttta aagcagaata gtattttacc ggaaaaattt
     3721 agttcctatc tggcacaaaa ggcatttgcc aaaacccggg attacgatgc tgaaattgcc
     3781 gcttattttg cctctcaagg aaaagaggaa tctattacta acgaactccc tgctcaactg
     3841 gagttttcta tttctttaaa gagaaaatta cgctatggcg aaaaccctca tcaaaatggt
     3901 ggtttctata caaatgtaaa tcccggttgg gaacttattc acggtaagga actttctttc
     3961 aataatataa tggatatgga ttcggctttc agggctattc gtctttttgc taaaccaact
     4021 gccattatta tcaaacattg caatccttgc ggtatcggca gtggagaaac attggcagag
     4081 gcatatagaa aagcttatga aacagatacg gaggcacctt ttgggggaat tgtaattgtg
     4141 aaccgtcctc tggatttaga aacagctacc ctgatcaata atatcttcac tgaaatcatt
     4201 atcgctcctg cttttgagcc caatgttttg gaatttctga aaaaaaagaa aaaccgccgc
     4261 ctaattcact acgaattttc tctgttggaa aaaccactta accctgtaga aattaaaaca
     4321 ttaacttgcg gctatctggc tcaggattgg gacctcgtaa acgaatcaac agagaactgg
     4381 aaaatagtta ctaacaaaca acctgctccg gaagagtggg aagccattat atatgcctgg
     4441 aaagccgttt caatcctaaa gtccaatgcc attgccatcg ctaaaaaaga tagggtatta
     4501 ggtcttggct gtggacaaac ctcccgcata gatgccgttc aattagctct ctggaaggca
     4561 aaaaaattcg gacacgattt aacggattct gtttgtgcct ccgatggctt tttccctttt
     4621 cgggattgta tagataccct tgctaagaac ggaatttcag ctatcatcca acctggcggt
     4681 tctaaaaacg atgaggaatg
//
LOCUS       DAOOZV010000094         4669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_15303_length_4669_cov_60.219983, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000094 DAOOZV010000000
VERSION     DAOOZV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15303_length_4669_cov_60.219983"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1197
                     /locus_tag="PLW08_07355"
     CDS             82..1197
                     /locus_tag="PLW08_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL52452.1"
                     /translation="MKKVLHIQLLPILSGVQRFSLHLLDGLDRNEFEIWIACKPGGEF
                     VDEIKVRGYHYLPLPTFRHQICVWDIFTFFHLLYLIKKHHFDIVHTNASKPGLLGRLA
                     ARLGKVPLIIHTTHTFPFLEHQKLVTYKFFTTLEKIGNRLGDYNVFVNNSDRLKSLRM
                     GLIKPEKAITIYNALPLGLTSALQEIAQNRKKPEKEIIIGSTLRFCLQKNVINLITAS
                     CNACLQEPKLKFIYLGEGEYFELCKAIVHSYGLEERIILPGWDKDVLPWLKVFNVFIL
                     YSRWEAMPFSIIEAMHSGLAVIVSDLPSLSELVDSETGYIVPLDDSANLIQTFREIAS
                     DFEMPYQKGKKAAKKIAKICSYEKMVNAYRELYLSAK"
     gene            1210..2256
                     /locus_tag="PLW08_07360"
     CDS             1210..2256
                     /locus_tag="PLW08_07360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPL52453.1"
                     /translation="MINIYLLIIIEAVLIHLCTHLLFPLNLKISKKMKLVAYPGERKI
                     HKIPIPEAGGLCFALPIILAQATFGLFSGNAEMGKMLLELSGVGFLALCFGVWDDRFE
                     SPPPYKLVWQICLGVIMYLFGYRVLYLTNPFGEHFILGWLSFPVTVLWYLIVINAINF
                     IDGLDGLACGITIIVSAVLIVIGIKEQNELVIAISSFLLAGNLAFLRYNFYPAKIFMG
                     ETGALFIGLNIAAISTAGTSQYKGITSITLMVPLAVMAVPLIDFVLAVFRRLHYRDMF
                     HSDKEHIHHTMLDIGFSQRAIAIIIYLVTLLFGLIAIGFSFSSQKIVFSLLLGLFALM
                     VVIAYLIMRQERKK"
     gene            2253..>4669
                     /locus_tag="PLW08_07365"
     CDS             2253..>4669
                     /locus_tag="PLW08_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52454.1"
                     /translation="MKRFFLLVTLLISVFLPAQNWKTFTNTNHIYDLCMKDNNLYVAT
                     WGGVLKISPIDNSNNLSAYTEKEIYHKGNGLVSNDIRTLSYIDFSESLWMGSSDNGIS
                     ILSQLGIQNLGSALGLPSLKVTRIIEHESTILVATSSGLAVFYYLPGVAFPLMLHQYT
                     VANTSGGLAGDNIVDMLLTETGTLFVATTNGVSYIPLELLDNDSAWKTISGPGSPLLS
                     GGNPKLSANSENIAIGIYKEVYVHSLNMDSGSWTTYNVSNMLTGKNVSSVLLDAQSRL
                     WISYADWDEAILSYTNTSDSLLTLIENNNYTHILKETNGLGYSPISRIVELNGEIYLC
                     SWGQGIYHQEENYWSNYDPTGIGFPRIAQVVTDNDYNLWFASGYISNLPVRKGSMGVS
                     KLTDGNWLTLNIHNSPIHTDNILGVAVDPLNRKWFATWDNTNSPSGWEKGLSVLDEEN
                     NIWVHITKDGIRYYNNETQSWGAYNPNPSYRLTTSTIGGIYPAGDSLMMVMCYDGAVN
                     VLDMNFQIVSRFSPPNTVFNHLLYGYYNGEKYFIGTNDDRGLTIWNHNSLPVSGGEHW
                     LIPPPADLSSCVVYGVVTIDTPYEGRQHWIAASTGVFMWNEVNWYRYDTMIKRYKYNI
                     FSHLWENDLLYYVDEERLFGSVRTIPTCIFLDAQNRIWLGSMENGLSLYDPYTERFTN
                     YFYPNYPLISNYITCLGFDPVAGDLIIGTPDGYNTLKIGRTVKIPTTLQNLKAFPNPF
                     RPSVQPYLQIVNMPVDNMPAGKAECRIYDSSGALVIKLEENALCRFQWDGKNAAGKQC
                     SSGIYFFV"
BASE COUNT         1346 a          769 c          950 g         1604 t
ORIGIN      
        1 agatttacag gatttagaat ataagttccg gaggaacgac agataataac gacggggaaa
       61 ttgttttcca gggattaaaa gatgaaaaag gtgttacata tacagttgct accaatacta
      121 tccggggtgc agagattttc tttgcatctt ttggatggct tggatagaaa tgaatttgaa
      181 atctggattg cctgcaaacc aggtggagaa tttgtggacg aaataaaagt taggggttat
      241 cattatttac ccttacccac ttttcgtcat cagatatgtg tttgggatat ttttaccttt
      301 ttccacttgc tttacttaat taaaaagcac cattttgata ttgtgcatac taatgcttcc
      361 aaacctggat tattaggtcg tttagctgct cgtttaggta aagttccttt aattattcat
      421 accacacata cttttccgtt tctggaacat caaaagctgg ttacctacaa gttttttacc
      481 actttggaaa agataggcaa ccgattgggt gattacaatg tttttgtgaa taattccgat
      541 cggctaaaat ccttaaggat gggtttaatc aagccggaaa aagcaatcac tatttacaat
      601 gcacttccgt taggattaac ttctgcttta caggaaattg cccaaaaccg caaaaaaccg
      661 gagaaagaaa ttattattgg ttctactttg cgtttttgtc tccaaaaaaa tgtaatcaat
      721 ttaataactg cttcgtgtaa tgcctgtttg caggagccaa aattaaaatt catctatctg
      781 ggtgagggtg aatattttga gctttgtaaa gcaatcgttc acagttacgg tttggaagaa
      841 cgcattatac ttccgggttg ggataaagat gttttgcctt ggctaaaagt tttcaatgtc
      901 tttattttat attcccgttg ggaagcgatg ccttttagta tcattgaagc aatgcattca
      961 gggctggcag taattgtttc agacctgccc tctctttcag aattggtaga tagtgaaaca
     1021 ggttatattg ttcctttaga tgatagtgct aatttgattc agacctttag ggaaatagca
     1081 agtgattttg aaatgcctta tcagaaaggg aaaaaagcgg caaaaaagat agccaaaatt
     1141 tgttcttacg agaagatggt taatgcttac agagaacttt atctatcagc aaaataacag
     1201 agcagcataa tgataaatat atacttacta ataataattg aagcggtgtt gattcacctc
     1261 tgcacccatt tgctttttcc gctgaatttg aaaatttcta aaaaaatgaa gctcgttgcc
     1321 tatccaggtg agcgcaaaat tcataaaata ccgattccgg aagcaggggg cttatgtttt
     1381 gctttgccaa ttattttagc acaggcaact tttggtttgt ttagtggaaa tgcagagatg
     1441 ggtaaaatgc ttttagaact ttccggagta ggatttctgg cattatgttt cggggtttgg
     1501 gacgatcgtt ttgaaagtcc tcctccttat aagctcgtgt ggcagatttg tttaggcgtt
     1561 attatgtatt tgtttggtta ccgggttttg tatctgacca atccattcgg agaacatttt
     1621 attttgggtt ggttatcttt tccggttact gttttatggt atttaatagt aatcaatgcg
     1681 atcaatttta tagacggtct ggatggtttg gcgtgtggca taacgattat tgttagtgcc
     1741 gttttgatcg taatcggtat caaggaacag aatgaactgg taatcgctat ttcttctttt
     1801 ttgctggcag gaaatttagc tttcctgcgt tataattttt atccggcaaa gatatttatg
     1861 ggtgaaaccg gtgctttatt tatcgggttg aatatagctg caatttccac tgccggaact
     1921 tcacaatata agggaataac ttctatcacc ctgatggttc ctttagcagt tatggctgtg
     1981 cctctaattg attttgtttt ggctgttttt cggcgtctgc attatcggga tatgtttcat
     2041 tccgataaag agcatattca tcatacgatg ttggatattg gtttttctca aagagcaatt
     2101 gcgattataa tatatttagt aaccttactt tttggactta ttgccattgg cttttctttt
     2161 tcctcccaaa agattgtttt ttccttactt ttggggcttt ttgcgttgat ggtagtaatt
     2221 gcctatttaa ttatgcgaca ggagcgaaaa aaatgaaaag gttttttctg cttgtaacct
     2281 tgctgataag tgttttttta cctgcacaga actggaaaac atttaccaat acaaatcata
     2341 tttacgattt atgtatgaag gataataatc tttatgttgc tacctggggt ggagtgctaa
     2401 aaattagtcc catagataat tctaataatt taagtgctta taccgaaaaa gaaatttacc
     2461 ataaaggtaa cggattggtt tctaacgata ttagaaccct ttcctatatt gatttttccg
     2521 aaagtttatg gatgggctct tcggataatg gaatttctat tttaagtcaa cttggtattc
     2581 agaacctggg aagtgcttta ggattgcctt cactaaaggt aaccaggatc attgagcacg
     2641 aatcaacaat tcttgtggca acttcatctg gacttgccgt tttctattat cttccaggtg
     2701 ttgcttttcc tttgatgctt catcaatata ctgttgccaa tacttctgga ggtctggcag
     2761 gagataatat tgtagatatg ttattaaccg agacaggaac tctttttgtg gctacaacta
     2821 atggagttag ctatattccc ttggagttat tggataatga ttctgcctgg aaaactatta
     2881 gtggacctgg cagtcctctt ctaagtggtg gaaatccaaa actttctgcc aattctgaaa
     2941 acattgcaat tggcatatat aaagaggtct atgtgcattc tttgaatatg gatagcggtt
     3001 cctggacaac ttataatgtg tctaatatgc taaccggaaa aaatgtctct tcagttctgt
     3061 tggatgcaca atcgcgatta tggatttctt atgccgattg ggatgaagca atattaagtt
     3121 acacaaatac aagcgattca cttttaacct taatagaaaa taataactat acccatattt
     3181 taaaagaaac aaatggactt ggttatagtc caatcagcag aatagtagaa ttgaacggtg
     3241 aaatttatct ttgcagctgg ggacagggta tctatcatca ggaagaaaac tattggagta
     3301 attatgatcc aaccggtatt ggatttccca gaattgctca agttgttact gataatgact
     3361 ataacttgtg gtttgccagt ggttatattt ctaaccttcc agtgcgtaaa ggttcaatgg
     3421 gtgtaagtaa attaaccgat ggcaactggt taaccttgaa cattcataac agtcctatcc
     3481 atactgataa tattttaggt gttgcagttg atcctttgaa cagaaaatgg tttgccacct
     3541 gggataatac aaacagtcct tccggttggg aaaaaggtct cagtgttttg gatgaggaaa
     3601 ataatatctg ggtgcatatc acgaaagatg gtatccgcta ttataataat gaaacacaat
     3661 cctggggtgc atataatcct aatccttcct atcgtttaac caccagcaca attggaggta
     3721 tttatcctgc cggagattct ttaatgatgg tgatgtgtta tgatggcgca gtaaatgttc
     3781 tggatatgaa tttccaaatt gtgtctcgtt tttctcctcc caatacagtt tttaatcatc
     3841 tcctttatgg ttattataac ggagagaaat attttatcgg aactaatgac gatcgcggac
     3901 ttactatttg gaatcataat tctcttcctg tttcgggagg agagcattgg ctaattcctc
     3961 cccctgcgga tttaagcagt tgtgttgttt atggagtagt tacaattgat acaccttatg
     4021 aaggaagaca gcattggatt gcagccagta caggtgtttt tatgtggaat gaagttaact
     4081 ggtatcgcta tgacactatg ataaaaagat ataaatataa cattttttcc cacttatggg
     4141 aaaacgatct tctctattat gtagatgaag aacgtctttt tggctcagta agaactatcc
     4201 ctacctgtat ttttctggat gcccaaaatc gtatctggct gggaagtatg gaaaacggac
     4261 tttctctgta tgatccttat acagagaggt ttactaatta tttttatccc aattatcctt
     4321 taatttccaa ttacataact tgtctgggtt ttgatcccgt tgctggggac ttaattatcg
     4381 gaactccgga tggttataat accttaaaaa tcggaagaac ggttaaaatt cctacaactt
     4441 tacagaattt aaaggcattt cccaatcctt tcagaccttc tgttcagccc tatttgcaga
     4501 ttgtaaatat gcctgtggat aatatgcctg cgggaaaagc agaatgtaga atttatgata
     4561 gttcgggtgc tttagtaatt aaactggaag aaaatgccct ttgccgtttt cagtgggatg
     4621 ggaaaaatgc cgcaggtaaa caatgcagta gcggaattta cttttttgt
//
LOCUS       DAOOZV010000095         4366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_16533_length_4366_cov_67.937833, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000095 DAOOZV010000000
VERSION     DAOOZV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16533_length_4366_cov_67.937833"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="rnhC"
                     /locus_tag="PLW08_07370"
     CDS             complement(<1..917)
                     /gene="rnhC"
                     /locus_tag="PLW08_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPL52455.1"
                     /translation="MKSQKIMHKELENYLSHLLPELQKTGISIDEQIEIPYGVQLKVS
                     RHNYKSILNIYYSQKKGISPVPTGNNNPLKKLLWDLCRLQEELKLDMPPMHTWNAWIG
                     SDECGKGDYFGPLVVCAFAYKRERENEFKIVGVKDSKKINSEEIKRIAKYLYKNYANC
                     IHCIVIKPEKYNELIAKFKAQKKNLNDLLAWLHSTAIIALQKNHPESQGVLVDQFSVS
                     HKTKYALQEKQFPLPCIERTGAEIDPAVAAASIIARYQFLEAFEAMRKFYQLNFPQGA
                     NGNVIKYAQDFIKQYGEKRLGEVAKLHFKT"
     gene            complement(892..2172)
                     /locus_tag="PLW08_07375"
     CDS             complement(892..2172)
                     /locus_tag="PLW08_07375"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPL52456.1"
                     /translation="MLYSEFLEHIFKRYSGNVKLELNRMQGLLNDMGNPERKLNGFHI
                     GGTNGKGSVCATLEALCRESGLNTALNTSPHLINYNERFRINGKEPSFETILNTFLRY
                     ENLFDKWEASFFEITTALAFSMFSEQNVDVSIIEVGLGGRLDATNLFVPDVAVITNIG
                     LDHIKTLGGTLEIIAGEKAGIIKEGIPLVLGDIEDSPRSIIEAVAKAKKVPVFRYKRD
                     WQTSIKNDNLTGINFDYSFGNYEFPNLEANLIGEHQAINIGSALTAFILYAEKKKLPV
                     AEEVIRKALQHISWKGRMQVLSQKPIIIIDGAHNVHGVKALMSTLEKIFPNRKVKFMV
                     SILGDKDYKQMLSLICPKAEQIYVARNQSERAASVEDQVAVIEEYNIPYKTAPTVAEA
                     YSLAVKECKEDDILIVCGSLFTVGEVLEIPKDNA"
     gene            complement(2284..2928)
                     /locus_tag="PLW08_07380"
     CDS             complement(2284..2928)
                     /locus_tag="PLW08_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52457.1"
                     /translation="MLRNQNGISVYTVLSIILFIALVFILALPNFFNLDKEKNLEDCI
                     NNMKQIWVATTDYMRDTNADFNGDLSLLIKTPKKDDPKNTYLSSNLYCPETSHQKKEY
                     LVYGKYIAEQIGTEIKHNYGIIILCPNLAQYPKHIIEKGFYENMEPTQLQNYMSEDID
                     YINSETGLNGAKKVELINKYIEIWKTDPDAFAKRKANPTALKAILFPEKFGITK"
     gene            complement(2984..3514)
                     /locus_tag="PLW08_07385"
     CDS             complement(2984..3514)
                     /locus_tag="PLW08_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPL52458.1"
                     /translation="MAKGKHRKNKKSNTGRIISILLVLFILLILVYNPISLRLTTIGV
                     AIYYGLNPVVFYRVIKTESSFRSFAISDAQAIGLGQIKESTAFYIHKDHKRGYLFLPV
                     YNLKLAAKYLVYLKNRYNGNWSLTLAAYNWGESNVNRRLKGIEIEKDKDYSSLFRDIP
                     ETYSYITKILYPKNKA"
     gene            complement(3654..4139)
                     /locus_tag="PLW08_07390"
     CDS             complement(3654..4139)
                     /locus_tag="PLW08_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL52459.1"
                     /translation="MAIYSVNEVIEMAVQIERSGYAFYNEAVKRKDLDEKAKDFLIYL
                     RDEELRHEKTFLALRDEVDLQVLQLSVDWELIAEYLKTIVESRIFSNEFSAIQLATKA
                     KDIKEIVENAITFEKDTLLYFHTLSDNINEPRTHNALRKIINEEVSHILKLSEMKKKL
                     V"
BASE COUNT         1303 a          795 c          764 g         1504 t
ORIGIN      
        1 gttttgaaat gcagtttggc aacttcgccc aagcgttttt caccgtattg tttaataaaa
       61 tcctgagcat atttgataac attgccatta gcgccttgag gaaaattcag ctgatagaat
      121 ttacgcattg cctcaaatgc ttccagaaat tgataacggg caattatgga agcggcagca
      181 acggcaggat ctatttcagc tccggttctt tcaatgcagg gaaggggaaa ttgtttttcc
      241 tgtaaagcat attttgtttt atgtgacaca ctgaattggt caacaagcac accttggctt
      301 tcaggatggt ttttctgcaa agcgataata gcagtggaat ggagccaggc aagcaaatca
      361 ttcaggtttt tcttttgggc tttgaatttg gctatcagtt cattatattt ttccggttta
      421 atcacgatac aatgaatgca gttagcataa ttcttataga gatatttggc aattcgtttt
      481 atttcttcgc tgtttatttt tttgctatcc ttcactccaa caattttgaa ttcattttcc
      541 ctctctcttt tataggcaaa ggcacaaaca acgaggggac cgaaatagtc accttttccg
      601 cattcatcgc ttcctatcca agcattccag gtatgcattg ggggcatatc caattttaat
      661 tcttcttgta aacggcacaa atcccataag agttttttta agggattatt atttcctgtg
      721 ggaacagggg aaataccctt tttttgtgaa taatagatgt tcagaatact tttataatta
      781 tgccgggaga cctttaattg aactccataa ggaatttcta tctgttcatc aatagagatt
      841 cccgtttttt gtaattcagg cagtagatga gaaaggtaat tttccagttc tttatgcatt
      901 atcttttggg atttcaagca cttcgccaac tgtaaaaaga gagccacaaa caattaaaat
      961 gtcatcctcc ttacattctt ttacagccaa agaataggct tcagcgacag taggagctgt
     1021 tttatacggt atattatatt cttctataac ggctacctgg tcttcaactg aagcagctct
     1081 ttcggattga ttgcgggcaa catagatttg ttccgcttta ggacatataa gagacagcat
     1141 ttgtttatag tccttgtctc ctaagatgga gaccataaat tttactttgc ggttggggaa
     1201 aattttctcc agagtggaca ttaatgcctt cacaccatgc acattatgag ctccatcaat
     1261 gattataatc ggcttttggg aaagcacttg cattctacct ttccaggaaa tatgttgtag
     1321 tgctttccga ataacttctt ccgcaacagg caattttttc ttttccgcat agaggataaa
     1381 agcggtaagt gccgaaccaa tattaattgc ctggtgttct cctattaaat ttgcttccag
     1441 attgggaaat tcataattgc caaaactata gtcaaaattt ataccggtta ggttatcatt
     1501 cttgatagag gtctgccaat cgcgtttata gcgaaaaaca ggaacttttt ttgcttttgc
     1561 cacagcttca ataatagagc ggggagaatc ctctatgtct cctaaaacca aagggatacc
     1621 ctcttttata ataccagctt tttccccggc aattatttcc aaagttcctc ccagggtttt
     1681 tatatgatca agtccgatat tggtaattac ggcaacatca ggaacaaaaa gattggtagc
     1741 atccaaacga cctcctaagc caacttcaat aatagaaaca tctacatttt gttcactaaa
     1801 cattgaaaaa gcaagagcag tagttatttc aaagaaagat gcttcccatt tatcaaacag
     1861 attttcatag cgaaggaagg tattgagaat tgtttcaaaa gaaggttctt taccgttaat
     1921 ccggaagcgt tcattatagt ttatcaaatg cggcgaagta tttaaggcag tgtttaatcc
     1981 actttcccta caaagagcct ccagggttgc acaaacactt ccttttccat tagttccccc
     2041 aatatgaaaa ccgtttaatt tgcgttctgg attacccata tcatttagca aaccctgcat
     2101 tctattcaat tccagtttca catttccgga atagcgtttg aagatatgct caagaaattc
     2161 actgtaaagc atcgggtcct tttacctgaa ttaaaagttg ttagctataa aaaaattcaa
     2221 acagcatcat aacttcaaga tgctgtttga atctcatatt aacctaaatt agaataatgt
     2281 tcgttatttg gttatcccga atttttcggg aaaaagaatt gctttgaggg cagttggatt
     2341 ggctttgcgt ttggcaaagg catcaggatc ggttttccag atttctatgt atttatttat
     2401 caattcaact tttttagctc catttaagcc agtttcggaa ttaatataat caatatcttc
     2461 agacatataa ttttgcagtt gtgtgggctc catattttca tagaatccct tttcaataat
     2521 atgcttgggg tattgagcca gattggggca gagaataata atcccgtagt tatgctttat
     2581 ttctgttccg atttgttctg ctatatattt tccgtaaaca agatattctt ttttctgatg
     2641 actggtttca ggacaataca aattggaaga caaataagta ttcttggggt catctttttt
     2701 aggtgttttt atcagcaatg aaagatcacc gttaaaatct gcattggtat cacgcatata
     2761 atcagttgtt gccacccaaa tttgtttcat attattgata cagtcctcaa ggttcttttc
     2821 tttatccaga ttaaagaaat tcggcaatgc caggataaat accaaggcaa tgaagagaat
     2881 gatactgaga acagtatata ccgagattcc attttggtta cgcagcataa atcactccta
     2941 aatgaattct aaaaattatt atttttctga tgtgttattc tcgtcaagct ttatttttgg
     3001 gataaagaat tttcgtgata taagaatagg tctcgggaat atctctgaaa agagaactat
     3061 agtccttatc tttttcaatt tcaatgccct taagacggcg gttaacatta ctttcacccc
     3121 agttataagc agctaaagtt aagctccaat tgccattata tctgtttttc agatagacca
     3181 aatattttgc tgccagcttt aaattataaa ccggtaaaaa gagatagccc cttttatgat
     3241 ctttgtgtat atagaaggca gtggattcct taatttgtcc caaaccaata gcttgagcat
     3301 cagaaatggc aaaactgcga aaagaacttt ccgttttaat tacgcggtaa aatacaaccg
     3361 gattaagtcc ataataaatt gctactccaa tagttgttaa cctcagagaa ataggattat
     3421 agaccaaaat aagcaaaata aacaaaacca gaaggatgct gatgatcctt ccggtattgc
     3481 tttttttatt ttttctgtgt ttacctttag ccattattgc ttgtttttta ttactgttct
     3541 tttgttcggc ttgttttttg tttattcttc ttgattgctc ctcaatttct gtaattttct
     3601 tttagttatt ccagattttt tctataatct atttatgcaa gaatacggga tagttacacc
     3661 agttttttct tcatctcgct cagtttcaaa atatgactta cttcctcatt gattattttc
     3721 cgtaaagcat tatgagttct gggttcgtta atattatcgg aaagggtgtg gaaatagagc
     3781 agggtgtcct tttcaaaagt aatcgcattt tccacaattt cttttatgtc tttggcttta
     3841 gtggcaagtt gaatggcaga gaattcattg ctaaaaattc tgctctctac aattgttttc
     3901 aggtattcgg caataagttc ccagtcaacg gagagctgta aaacctgaag atctacctca
     3961 tctctcaaag ccaaaaaggt tttctcatgt ctcaattctt catcccgtaa ataaattaaa
     4021 aagtcctttg ctttttcatc aaggtctttt cttttaactg cctcattata gaaagcataa
     4081 ccgcttcttt caatttggac tgccatttca attacttcgt ttacagaata tattgccatt
     4141 ttttcctccc tggaaatttt tttattccag tttatgatag caaagagaat agtcaagagg
     4201 aaaatgcgga aaaagcagga tggcaaagta acaataccag aaggttgatg tccttattaa
     4261 ccactcgcaa caaagaattg agagagttga ggaaatgata aatgaaaaat gaagaatgaa
     4321 tttaacaagg aaattaggct aaaattggaa tttttaaggt ttatac
//
LOCUS       DAOOZV010000096         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_17215_length_4224_cov_73.112257, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000096 DAOOZV010000000
VERSION     DAOOZV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17215_length_4224_cov_73.112257"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..130
                     /locus_tag="PLW08_07395"
     tRNA            55..130
                     /locus_tag="PLW08_07395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ala,seq:cgc)
     gene            947..4144
                     /locus_tag="PLW08_07400"
     CDS             947..4144
                     /locus_tag="PLW08_07400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL52460.1"
                     /translation="MARLFAKDYHNKSDSWYFNQAIIVLEKDDLWFWKTRNEDGNYQI
                     RFFPAPSKQEYFAEYVDIVFNPETELIVSHDCSQCHTDESCRHYLSILRYAYLNLSTR
                     IFEEEVVETCDGNALSGNELWLNIARNAVLYVEGIYNPETDKIRFYHQDLAPIDPILM
                     GKLYSQEEINDIPQRQIEYYTANMGAFRDADLHLFSFLNKNRCAYSHKNKFWSIYKSD
                     FSQALLIMQNCHTRFVIRETGEELIFQQQPYQLALRIEPSGTKNYSLRAVIIDELSVW
                     FAGNPTWLFFRNRVYKINLPFRKEIIDHIFGSGEVLTARDLIYYRCLVHRELQEQGIY
                     LDFEESISLPEIIDETPVKRLHIKKLGDKILIGGSLVFSQEREIPLSVLRFGKPLVYS
                     QYTSPDKNGSAWFHLTPELFAKTQELLNKLPEPEMNRLEQYAELVFSGKERIEQLRTA
                     IFELSDQDWDIQIDPELGNYFVAKIPLQVELIARHEEDIDWFTYEVRYHYRDLNFTHE
                     ELKRFFRSKEEFLHTSDGRLVFISNPQVFYEVEELLSRSEHKTDQVYRSRMMNLPYYL
                     KLREDNPAFRMQGDEWLEGFYEALLKRRLEGPETLPLYLQTVLRGYQKAGVAWIKMLA
                     HYHLNGILADEMGLGKTIQALSAILSTTLGQVSLVICPKTLLYNWAAEIDKFHTNIPF
                     AIVDGNKTTRMEILSNPNVQLFIMSYSMVLGDVTYLKNIEFEWIVLDEAQNIKNVSAQ
                     RTSAIKKLKSKHRLALTGTPIENNLTELWSIFDFLNPGYLGTLNKFKQNYLPAEGEIT
                     ARLSLSRMVAPFMLRRIKKDVLLELPDKQEQISWCKLTTLQEKLYLQILDMVHKKLLP
                     EGKEMPSYIHILAALTKLRQVCNHPHLANGDILPELEASSKLEQLLELVTEATNAGHK
                     VLIFSQFVQMLSIIRKVLEAKSLPYCYLDGQTKDRVTPIKSFETNPEIKLFLISLKAG
                     GTGLNLTAADTVILYDPWWNPMVENQAIDRTHRIGQTHKVQVFRLITKGTVEEKILQL
                     QQNKRELFETVIEGGQNVLKAMTKEDIKKLFSYSE"
BASE COUNT         1286 a          814 c          836 g         1288 t
ORIGIN      
        1 tcacattgct cttgacaaaa ccctatctct taaaatttat tcctaaagtg cgttggggct
       61 atagctcagt tggtagagcg aatggttcgc aatcattagg ccaggggttc gaatcccctt
      121 agctccacca caaattttat ttcttacggc aagagagtgg ttatattaca tatatccatt
      181 atcaattaag ttccgtttct ctgaagcgaa agaatactgt ccacttacga aagaacaaat
      241 taccctttat aaagcagtag cggatgattt gccaaataaa gaaggaattg aaagaaaggg
      301 acttatttta actgcacaga aatgaatctg gagcgtcctc aactccattc tgcaattatt
      361 aaacgcttgg gaaatattac tttctggcgt tctaaaatca attttaggga caaaatggag
      421 gaaatttata ccagagtcgg gaaaaaagca gaaaacatat tagaacaata agttacataa
      481 ttttttgtcc ttcttccctc aatttttata actctctttc ttgcctccat taccttagca
      541 ttgcttaccc aatactaatg ccatccgtaa tgggtaagca atgggtaagc aatgctatta
      601 aggacgaaat cggaattttc tctttttcaa ctaattaccc acacgcagaa gaaaaaataa
      661 tgagggtgcg attttccttt cccttttggg ggattaacga tgtcttattt tccctaaatt
      721 acaagaacat atctgtgtta tctgtgcaat ctatgagaga ttaatttcat taatttctgt
      781 ggtgataaca tctttcttct cctcttttct ctttatttac aaatcattct tgatgttttc
      841 agtgttctct gtgctctcac tgtaaaaaaa cccctttttt agattgacag attttccctt
      901 tttaccctat aggaactgat taaaaaataa aaggatactc aaactgatgg cacggctttt
      961 cgctaaggat tatcataaca aatcggatag ctggtatttc aatcaggcaa taattgtgct
     1021 ggagaaagac gatttatggt tttggaaaac ccgcaatgaa gatggcaact atcaaattcg
     1081 ttttttcccg gctccttcca aacaggaata ttttgccgaa tatgtagata ttgtttttaa
     1141 tccggaaacg gagctgattg tttcccatga ttgttcacag tgccatacgg atgaatcctg
     1201 tcgtcattat ctttccattt tgcgttatgc ttatctgaat ttaagcaccc gcatttttga
     1261 ggaagaggta gttgagacct gtgatggtaa tgccctcagt ggcaatgaat tatggctaaa
     1321 tattgcccgc aatgcagttc tatatgtgga gggaatttat aatcccgaaa ccgataaaat
     1381 ccgtttttat catcaagacc ttgctcctat tgatccaatt ttaatgggta aactctattc
     1441 tcaggaagaa ataaacgata ttccacaacg ccaaattgaa tattatactg ccaatatggg
     1501 agcttttaga gatgccgacc ttcatttatt cagtttttta aacaaaaacc gctgtgctta
     1561 tagtcacaaa aacaagttct ggtcaattta caaaagtgac ttttcccaag ctcttttaat
     1621 tatgcaaaat tgtcataccc gttttgtcat tagagaaaca ggtgaagagc ttatttttca
     1681 gcaacagccc tatcaacttg ccttaagaat tgaaccctcc ggaacaaaga actattcttt
     1741 acgcgctgtt attatagatg aactctccgt ttggtttgcc ggaaatccta cctggctttt
     1801 tttccggaat cgggtgtata aaatcaacct gcctttccga aaagaaatta tagatcacat
     1861 ttttggcagt ggagaggttt taactgccag ggatttgatt tattatcgct gtctggtgca
     1921 tcgtgaactg caggaacagg gaatttatct ggattttgag gaaagtatat cgctgccgga
     1981 aataatagat gaaacccctg ttaagcgttt gcatatcaag aaattgggtg ataaaattct
     2041 aattggcggt tccctggttt tttcccaaga aagagaaatt cccctttccg ttttgcgttt
     2101 tggtaagccc cttgtttatt ctcaatatac ttctcctgat aaaaatggta gtgcttggtt
     2161 tcatttaacc ccggagcttt ttgccaaaac acaggaactg ctgaataaac ttcccgaacc
     2221 ggaaatgaac cgtttggaac aatatgccga attggttttt agcggaaaag aaagaattga
     2281 acaattgcgg actgctattt ttgaacttag tgatcaggat tgggatattc aaattgaccc
     2341 ggaattgggg aattactttg tggctaaaat tcctttgcag gtggagctta ttgctcgtca
     2401 cgaagaagat attgactggt ttacctatga agtccgttac cattatcggg atttgaattt
     2461 tacgcacgaa gaactgaaac gctttttccg cagtaaagag gaatttttgc atacctcgga
     2521 tggacgcctt gtgtttatca gtaatccgca ggtcttttat gaagtggaag aactcctttc
     2581 ccgcagtgaa cataaaactg atcaggtgta tcgttcccga atgatgaacc tgccctatta
     2641 tcttaaattg cgggaagata atcctgcctt tagaatgcag ggtgatgaat ggttagaagg
     2701 attttatgag gcgttactga aaaggcgatt ggaaggtcct gaaaccttac ctctctattt
     2761 gcaaaccgtt ttacgcggtt atcaaaaagc cggtgttgcc tggattaaga tgttggcaca
     2821 ttatcactta aatggtattc ttgccgatga aatgggcttg ggaaaaacca ttcaggcact
     2881 ttctgccatt ttatctacaa ctttggggca ggtctcactg gtaatttgtc ctaagacctt
     2941 gctttataat tgggctgcgg aaattgacaa gttccatacc aatatccctt ttgccattgt
     3001 ggatggtaat aaaacaaccc ggatggaaat cctctccaat cctaatgtcc agctctttat
     3061 tatgagctat tctatggttt tgggagatgt tacttacctg aaaaatatag agtttgaatg
     3121 gattgttctg gatgaagcac aaaatattaa aaatgtttcc gctcaacgca cttccgccat
     3181 caaaaaactg aaaagcaaac accgtttggc tttaacggga actcctattg aaaataatct
     3241 taccgaactt tggtctatat ttgatttctt gaaccctggc tatttgggca ccctgaataa
     3301 atttaaacag aattatctgc ctgctgaagg agaaataact gcccgacttt ctttatcccg
     3361 aatggttgcc ccttttatgc tgcgcagaat aaaaaaagat gtgctcctgg aacttccgga
     3421 taaacaggaa caaatttcct ggtgcaaact aactaccctg caggaaaaac tttacctgca
     3481 aattttggat atggtgcata aaaaactcct gccggaggga aaagaaatgc caagctatat
     3541 tcatatttta gctgcactta caaaactaag acaggtctgc aatcaccctc atcttgccaa
     3601 tggcgatatt ttaccggaac tggaagcatc ttccaaactg gaacagcttt tggaactggt
     3661 tacggaagcg acaaatgcag gacataaggt cttgattttc agtcagtttg tccaaatgct
     3721 gtccattatt cggaaggtct tggaagcaaa gtccttgccc tattgctatc tggatggcca
     3781 aaccaaggat agagttactc ccataaaaag ctttgaaacc aatcccgaaa ttaaactctt
     3841 tttgatctcc ttgaaggcag gtggaacggg acttaactta actgctgcag ataccgttat
     3901 cctatatgat ccctggtgga atccaatggt ggaaaatcag gcaatagata gaactcacag
     3961 aatcggacaa acacataagg ttcaagtttt tcgtctgatt accaaaggca ctgtggaaga
     4021 aaaaatcctg caactgcaac aaaacaaacg cgaactcttt gaaacggtta ttgagggtgg
     4081 acaaaatgtg ctgaaggcaa tgacgaaaga agatataaag aaacttttta gctattcgga
     4141 atagggtcaa gaggtcaaga gatcaatagg tctggaggaa agtgaaaagg ggaaaagggg
     4201 aaacccaagg caagcatcgt aaaa
//
LOCUS       DAOOZV010000097         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_18145_length_4043_cov_62.920512, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000097 DAOOZV010000000
VERSION     DAOOZV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18145_length_4043_cov_62.920512"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLW08_07405"
     CDS             <1..541
                     /locus_tag="PLW08_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPL52461.1"
                     /translation="KLLTESRVVKKLLNVNIKRKKRVERPSWQQYFMEMAYLASKRST
                     CLRRKVGAVLIRDNQIIATGYNGSPKGVPHCEDIGCLREQQKVPSGKNQELCRGVHAE
                     QNAIIQAAINGSSTRGAILYCTNQPCSICARLIINAEIKTVYIAESYPDELGEELFRE
                     AGVELIKFDLQSNTLKKLI"
     gene            541..1080
                     /locus_tag="PLW08_07410"
     CDS             541..1080
                     /locus_tag="PLW08_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPL52462.1"
                     /translation="MSIFRRFTVKDLIIITSIAALGIAIKPIINPVTKLISTPLGIPG
                     GSITGGLYMLWLVLAVVIVDKPFTGTLYGLLQSILVLLIGMAGKMGAFSLVAYTAPGI
                     VADLLFWLLPYKDKLITHLFLCGCANLTGSLISSAIFFQMPTVMIVFNTCMAFSSGIL
                     GGYLGFGIYQSLKAARIIQ"
     gene            1077..2003
                     /locus_tag="PLW08_07415"
     CDS             1077..2003
                     /locus_tag="PLW08_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52463.1"
                     /translation="MKKYLCLLLLSGVMITLSSLEVIDTKGISHFYNNAELYKKEMQE
                     IKTSREKDGTVRLNTWQGFRFDIWLKEQNLGDFATIRFESSDRYQVTLEKSEFDSLES
                     YLITGQDGIPFEENMLRLIFPALREMQWIKGLERVVLEDFQPLIRPQRFYLLQETLKK
                     YPLQQDPEPFVKTQGWYLRDILSDLSEAEEKQVILYSRDGLKQHLTYPLHLSGAILEK
                     TAEQHYNLKSPQIPGGMWMKDVIYLQCDNNALISEDSINQLIRLAKILHWETTPEMQF
                     RIVFEDREEVMNFTDALAEPQVFRGALYLELF"
     gene            2003..3352
                     /locus_tag="PLW08_07420"
     CDS             2003..3352
                     /locus_tag="PLW08_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL52464.1"
                     /translation="MLFLQDFGISFAEQKLWEGINLKIPEGSIFHLKGPNASGKSSLL
                     NAISGIIPEYVKAKTTGNILLGQTDLQAIPLKEKFHYLWHQLSDAEAQLFFPDCLSEL
                     AFALEHLALAPENIVAKINLAAAQFGLEKMLQRDPSTLSGGEKKLLLCAVAETIDPPL
                     LLLDEPLNGLDKQATNLVFKWLKNKKEQGKIIVVADHNPLMETVADFSYTLPENPQPL
                     TIDNSLFTEAVLNLQDKRDCAIKLIPDVLYNIEELSFAYPNAEFLFKDLSIKINSTEN
                     ILLKGKNGAGKSTFLKLLTGLLKPNKGKIYLQGKHLQGLDPKNFAHFYYQSQITKENL
                     LGISVKQNWLFWQLAIPELKDLPIKEDPLFTELSAGQQKMVSQQILPYMLSKFWILDE
                     PFASLDENAGKQLFNLLQYKSRNYPGMLIVSHSLEAHRELFDRVFSFQNQTVQEEKG"
     gene            3349..>4043
                     /locus_tag="PLW08_07425"
     CDS             3349..>4043
                     /locus_tag="PLW08_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPL52465.1"
                     /translation="MKKSSLHPLTHILIALLLSTFVFIAKKPVSLLCLTILALLYAVW
                     RMENGWKKVFKTLWHTLPFLLVLAFFQIVFRRTGTLLWSYGILKISEEGVLISLQLTL
                     RLLTVIYCAKALAVMDYTEFSKAFSAIRLPEELSFMLSYAIHLVPKFLGQLKGFVTGL
                     KLRGIDAADLSFSKRLQVYKQLSITALAELIRSSETAAIALELRGFRSEGKRSKLHRQ
                     KFSYRDLLCLLFFS"
BASE COUNT         1207 a          717 c          847 g         1272 t
ORIGIN      
        1 aaagctattg acagaaagca gggtggttaa aaaattgcta aatgttaata taaagaggaa
       61 aaaaagagtg gaaagacctt cctggcaaca gtattttatg gaaatggcat atcttgcttc
      121 caagcgtagc acttgtttac gcagaaaagt tggagctgtt ctcattcgtg acaatcaaat
      181 tatagctact ggctataatg gcagtcctaa aggagttccc cattgtgaag atattggttg
      241 tttaagagaa cagcaaaaag ttccttcggg caaaaatcag gaactttgcc gaggagttca
      301 tgctgaacaa aatgctataa ttcaggcagc tatcaatggt tcttctactc ggggtgccat
      361 tctttactgt accaatcagc cctgcagtat ctgtgcccgt ttaattatta atgccgaaat
      421 taaaaccgtt tatatagctg aatcctatcc tgatgaatta ggggaagaac tctttagaga
      481 ggcaggtgtt gaacttatca agtttgattt gcagagtaat accttaaaga aactaatctg
      541 atgagtattt tccggcgttt tacggtaaag gacttaatta taatcacttc catagccgct
      601 ttgggaattg ccataaaacc gattatcaat cctgtaacca agttaatttc cacacctttg
      661 ggaatacctg gcggttcaat taccggtgga ctttatatgt tatggttagt tttagcggtt
      721 gtgatagtag ataaaccgtt cactggaacg ctttatggtc ttttacagtc aattttggta
      781 ttacttattg gaatggcagg aaaaatgggt gctttttctt tagtggcata tactgcaccg
      841 ggaattgttg cagacctgtt attttggctt ctgccctata aggataagct gataactcat
      901 ttgtttctat gcggttgtgc caatttaacc ggctctctta tttcttccgc tatttttttt
      961 cagatgccta ctgtgatgat agttttcaat acttgtatgg catttagttc cggtatttta
     1021 ggtggctatc ttggttttgg catttatcaa tcgctgaaag cagcaaggat tattcaatga
     1081 agaaatattt gtgtttatta ctcttatcgg gagttatgat tactctatcg tctttggaag
     1141 taatagatac aaaaggcatt agccattttt ataacaacgc tgaactttat aaaaaggaaa
     1201 tgcaggaaat aaaaacctct cgcgaaaaag atggaactgt gcgtcttaac acttggcagg
     1261 gttttcgctt tgatatttgg ctaaaagagc aaaacttagg tgactttgct accatccgtt
     1321 ttgaatcctc agaccgctat caagtgaccc tggaaaaaag tgaatttgat tctctggaaa
     1381 gttacctaat tacgggtcag gatggaattc cttttgagga aaatatgctg cgcttgattt
     1441 ttcctgcttt aagagaaatg caatggataa aaggtttgga gcgtgttgtg ctggaggatt
     1501 ttcaaccgct aattcgtccc cagcgttttt atttgctgca ggagaccctg aaaaaatatc
     1561 ctttacagca agaccccgag ccctttgtaa aaacacaggg ttggtatctc agggatattt
     1621 tgtcggattt gtcggaagcg gaagaaaaac aggtaatttt atacagtaga gatggcttaa
     1681 aacagcattt gacctatcct ttacatttgt caggggcaat tctggaaaaa acagctgaac
     1741 agcattacaa cttaaaaagt ccgcaaattc caggtggaat gtggatgaaa gatgttattt
     1801 atctgcaatg cgataataac gctctaatta gtgaggattc tatcaatcag ctgattcgtc
     1861 tggcaaaaat attgcattgg gaaaccacac cggaaatgca atttaggatt gtttttgaag
     1921 atagggaaga ggtgatgaat tttacagatg ccttggctga accgcaggtc tttaggggag
     1981 cgctttactt agagcttttc tgatgctttt tttacaggat ttcggcattt cctttgcaga
     2041 acagaaatta tgggaaggta tcaatctaaa aattcctgag ggctctatat ttcatttaaa
     2101 aggacctaat gccagcggga aaagttcctt gttgaatgcc attagcggta taattccgga
     2161 atatgtaaaa gccaaaacaa cagggaacat tcttttgggg caaacggatt tacaagcaat
     2221 tcccttaaag gagaaatttc attatctatg gcatcagtta agcgatgcgg aagcacagtt
     2281 attttttccc gattgcctat ccgaacttgc ttttgctttg gaacatcttg ccttggctcc
     2341 ggaaaatatc gttgccaaaa taaacctggc agcagcacaa tttggactgg aaaagatgtt
     2401 acaacgcgac ccttcaactc ttagcggagg cgagaaaaaa cttttacttt gtgctgttgc
     2461 ggaaacaatt gatcctcctt tactgctttt ggatgaaccc ttaaacggat tggataaaca
     2521 ggcaaccaat ttggtcttta agtggctgaa aaacaagaaa gagcaaggta aaataatagt
     2581 agtagctgat cacaatccgt taatggaaac agtagctgat ttttcctata ctttaccgga
     2641 aaatccgcaa ccgctgacta tagacaattc attatttacc gaggcagttc tgaatttgca
     2701 ggataagcgg gattgtgcta taaaacttat tcctgatgtt ctttacaata ttgaagaact
     2761 cagttttgct tatccgaatg cggaattttt gtttaaagac ctctctatta agataaacag
     2821 cacggaaaat attctactga aagggaaaaa tggagccggg aaaagcactt tccttaaatt
     2881 gctaactgga ttgctaaagc ccaataaggg gaaaatatac ttgcagggaa agcatctgca
     2941 gggtttagac cccaaaaact ttgctcactt ttattaccaa agccagatta ccaaggaaaa
     3001 ccttttaggg ataagcgtta aacaaaactg gctattctgg caactggcaa ttccggaact
     3061 aaaggattta cctataaaag aagacccttt gtttacagag ctttctgccg gacagcaaaa
     3121 aatggtttcg caacaaattt taccctatat gttatctaaa ttttggatat tggatgagcc
     3181 ctttgcttct ttagatgaaa atgccggcaa gcaactgttt aatttactgc aatataaaag
     3241 ccgaaactat ccgggaatgc tgattgtttc acattctctg gaagcgcata gggaactttt
     3301 tgatagagtg ttctctttcc aaaatcaaac agtgcaggaa gaaaagggat gaaaaaaagt
     3361 tctttgcatc ctctaactca tatcttgata gctttgttgc tttccacttt cgtttttatc
     3421 gccaaaaagc cagtttcttt gctgtgcctt actattttgg ctttgcttta tgccgtatgg
     3481 aggatggaaa acggctggaa aaaagtattc aagacacttt ggcatacctt gcccttttta
     3541 ttggttttag ctttctttca aattgttttc cgacgcacag gaacattgct ttggtcttat
     3601 ggaatactaa aaatcagcga ggagggagtt cttatttctc tacagctaac tttgcgttta
     3661 ttaaccgtta tttattgtgc caaggcatta gcagtaatgg attataccga attttccaaa
     3721 gcattttcgg ctattcgttt acccgaagag ctatccttta tgctttctta tgctattcat
     3781 ttggtaccga agttcttagg gcagttaaaa ggatttgtta ccgggcttaa attgcgagga
     3841 attgatgctg ccgatttatc tttttccaaa cgcttacagg tttataaaca attgtccatt
     3901 acggctttgg cagaactgat cagaagcagt gaaacagcag ctattgcttt ggaattaaga
     3961 ggttttagaa gtgaaggaaa acgaagtaaa ctgcatcggc agaagtttag ctacagggat
     4021 ttattgtgtt tgctgttctt ttc
//
LOCUS       DAOOZV010000098         3995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_18400_length_3995_cov_49.924873, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000098 DAOOZV010000000
VERSION     DAOOZV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18400_length_3995_cov_49.924873"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..3621)
                     /locus_tag="PLW08_07430"
     CDS             complement(208..3621)
                     /locus_tag="PLW08_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPL52466.1"
                     /translation="MSKGPFMKKMAILPAIFLLLSFSLSAELIAEGNSKNSVSLLIDN
                     ESETILEYRIGEFEKNMITIEGEEWYQIRLPKEGITQDKGYPELPVFNRSIIIPDQAL
                     MAIEVFDLEFKDYPIKVAPSKGVITRDINPATIPYTFDNVYQENSFYPQTMVALSEPY
                     ILRDFRGITVLTTPFAYNPVTGTLRVYTSYKVKVYNQGNDTVNTFNRFRTSVSRSFSP
                     LYENHFLNWNSYRYTPVDDSYGKLLVICHSSFLTQIAPYINWKRQKGIDTELVEFSTI
                     GTTANQLKAYIQNRYNADNSITFVQLVGDAPQIPTLSYSGGGSDPGFSLVAGSDNYPD
                     IFIGRFSAQTTAEVTAQVNKTIQYEKDLNTSATWLSKAMGIASAEGTSGEGDNGESDI
                     THMNLIRTKLLNYGYTSVDQIYDPGASASTVTTNVNTGRGFINYVGHGSNTTWGTTGF
                     SNTNASALTNGNKVPFIMDVACVNGNFVSYTCFAEAWMRNANGGAVGIYASSINQSWA
                     SPMRAQDEVTDLLTGEVHTTLGGLYYNGSCKMMDVYGYDGVDMFKTWHIFGDASLQVR
                     SKTPLAMTVSHPASVTSGTNNIIISSGVANALVAITYNNTIYGKAVTNSSGSAIVNIS
                     NPPSGEITYTVTVTAFNRVTYTGSLQQVNVPPDQPRFVAEWEPAQGSIIRYPFGLPYS
                     LIADLSNNCLLYVIVSSSLQATCNSALVSNGVNMANVRYIVTDNDTYWIRDYGPWTIM
                     NRENNLQLIDFTYNRPRPYDDAVPLAIANFLGVDLYKMNITHTGGNIMTDGQGKAMST
                     DLVLTENTSLSQNDINIMFNNYLGVTDYQLYPDPNNTYINHIDCWAKLLDVDKVIIRS
                     VPTNHSQYNAIEAVVTQWETKLSSYGTPYKIYRVYTPNDEPYTNSFILNKHIYVPLMG
                     TANDTSAILAYQNAMPGYIVTGYLYSSFESTDAIHCRVNTIFDDKMISLKHIPPVLSP
                     VAQSLTLSANITHTNALSPSATYISWKIGKNGIWQNSNLTLQSDGNWVTNLTAPVIGD
                     TLFYCFSVTDVTNRNVTLPLCAEYDPFMLIGVYVPAISQPQVQLQVQDANVRISWNAI
                     PYANNYVIYSANSPDEVFLPVGSTSATFWETPLNTEKKFFKVVAQRVVR"
     gene            complement(3682..>3995)
                     /locus_tag="PLW08_07435"
     CDS             complement(3682..>3995)
                     /locus_tag="PLW08_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPL52467.1"
                     /translation="EEIPVEIETEEEKIETEEIPVEIEAEEEKAAEEIPVEPVIPEPE
                     HIEPEEITIIKETSEEERESEKTEKEREESETIVIPVTDETEEKKSEPLATQEETKKE
                     D"
BASE COUNT         1242 a          771 c          719 g         1263 t
ORIGIN      
        1 gtccaaagcg gatggaatct tctgttaccc gttttttctc gggattttcc tttcgctgta
       61 aataatttta agccaccgga aactcatcct gctctcttcg gtgaaaatgt agcggaaaaa
      121 ataatgctgc tgctggaaaa taaatagcaa ttgccatcgc tgttttattc caaacattca
      181 gtaaagaaaa ttctgaaaaa actattttta tcttacaact ctttgagcta ctactttaaa
      241 aaactttttc tccgtgttta aaggtgtttc ccaaaaagtt gcagaagtag aaccaacagg
      301 caggaaaact tcatccggtg aatttgcaga atagataaca taattattgg cataaggaat
      361 tgcattccag gatattctaa cattggcatc ctgaacttgc aattgcactt gtggttggct
      421 aattgcaggc acataaactc cgattaacat aaaaggatcg tattccgcac aaagaggtaa
      481 agtaacatta cgatttgtta catccgttac cgaaaaacaa tagaaaagag tatcaccaat
      541 aaccggagcc gtaagattag ttacccaatt tccatcactt tgtaaagtca agttactatt
      601 ttgccagatt ccatttttcc cgattttcca agaaatgtat gttgcacttg gacttagagc
      661 attagtatga gtaatattgg cactcaatgt aagggactga gcaacgggag aaagaactgg
      721 aggaatatgc ttaagagaaa tcattttatc atcaaaaata gtgttaaccc tgcagtgaat
      781 ggcatctgtg gactcaaaac tgctgtataa atatccagta acaatataac ctggcattgc
      841 attttgatat gcaagaatag cagaagtatc attagcagtc cccattaaag gcacataaat
      901 atgcttattc aaaataaagg aattggtata gggttcatca ttaggagtat agaccctata
      961 aattttatag ggagtgccat agctggaaag ttttgtttcc cattgagtta ctactgcttc
     1021 aatagcatta tattgcgaat ggtttgttgg cacactgcga ataataactt tatctacatc
     1081 cagaagcttt gcccagcagt caatatggtt tatatatgta ttattaggat cgggatacaa
     1141 ttgataatca gtaactccaa gatagttgtt aaacataata tttatatcat tttgactgag
     1201 ggaagtgttt tccgttaaaa ccaggtctgt actcattgct ttcccctgtc catcggtcat
     1261 aatattaccg cctgtatgag taatattcat cttatataaa tctactccca ggaaattagc
     1321 tattgccaaa ggaacggcat catcataagg acgaggacga ttataggtaa aatcaatcaa
     1381 ttggagatta ttctccctat tcataatagt ccaaggtcca taatctctta tccaataggt
     1441 atcattatcc gtaacaatat aacgcacatt agccatatta acaccattgg aaacaagagc
     1501 tgaattacag gttgcctgaa gtgaactgga tacgatcaca tacaaaagac aattattaga
     1561 taaatcagct atcagagaat agggcaaacc aaaaggataa cgaattattg aaccttgagc
     1621 aggttcccat tctgctacaa aacgaggttg gtctggagga acattaacct gttgtaagga
     1681 accagtgtaa gttactctat taaaagcagt aaccgttact gtatatgtaa tttctcctga
     1741 tggaggattg ctgatattaa caattgccga accactacta ttggtaaccg ctttgccgta
     1801 aatagtatta ttataggtta ttgccacaag agcatttgct acccctgaag agataataat
     1861 attatttgtt cctgaagtta cggatgcagg gtggctaact gtcattgcca gaggggtctt
     1921 tgaacggacc tgaagggatg catctccgaa aatatgccag gttttaaaca tatctacacc
     1981 atcataccca taaacatcca tcattttgca ggaaccgtta taatacaaac cacccagggt
     2041 tgtatgcact tctccagtta agaggtctgt aacttcatct tgagctcgca taggagaagc
     2101 ccaggattga tttatagaac tggcataaat tccaactgct cccccattgg catttctcat
     2161 ccaggcttca gcaaaacagg tataagaaac aaaattaccg ttcacacagg caacatccat
     2221 aataaaggga actttattgc cattagtaag agctgaagca ttagtattgc taaaaccagt
     2281 tgttccccag gtagtattgg aaccatgacc tacataattg ataaaacccc gcccagtgtt
     2341 tacattagta gtaaccgtgg aagcagaggc acccggatca taaatttgat ccacactggt
     2401 gtagccataa tttagcaatt tagtccgaat caaattcata tgggttatat cactttctcc
     2461 attatcacct tcgccactag ttccttcagc agaagcaatt cccattgctt tggatagcca
     2521 agtagccgaa gtattcaagt ccttttcgta ttggattgtt ttattcactt gcgctgttac
     2581 ttctgctgta gtttgagctg aaaaccttcc aataaaaata tcgggataat tatcactacc
     2641 tgctacaaga gaaaaaccgg gatcacttcc tcctccgcta taagaaagag ttggaatttg
     2701 aggtgcatca ccaaccagtt gcacaaaggt aatagaatta tctgcattat agcgattttg
     2761 aatgtaggcc ttaagttgat tggcagtagt tccgattgtg ctgaattcca ccaactcggt
     2821 atctatccct ttctgccttt tccaattaat atagggagct atctgagtta aaaaacttga
     2881 atgacagata accagtaatt taccgtagga atcatctaca ggagtataac gataggaatt
     2941 ccagttcaaa aaatggtttt cataaagagg ggaaaaacta cgggaaacag aagtccggaa
     3001 acggttgaaa gtgtttacgg tatcatttcc ttggttataa actttcactt tataagaagt
     3061 ataaactctt aaagttcccg taactggatt ataggcaaaa ggagtagtta aaacggttat
     3121 cccacggaaa tcccttaaaa tatagggctc tgataaagca accattgttt gaggatagaa
     3181 agaattttct tgatagacat tgtcaaaagt gtaaggaatt gtagccggat ttatatcacg
     3241 cgtaataact cctttggaag gagctacctt gatgggatag tccttaaatt caagatcaaa
     3301 aacctcaatc gccatcaatg cttgatccgg aataataata ctgcggttaa aaacaggcaa
     3361 ttcgggatag cccttatctt gagttattcc ttctttaggc agacgaattt gataccattc
     3421 ctcaccttct atagtgatca tatttttttc aaattcacct attcggtatt ccaggatggt
     3481 ttcgctttca ttatcaataa gcagggatac ggaattttta ctattaccct cagcaatcaa
     3541 ttctgcactt aagctaaaag acaggagcag aaatatagct ggtaatattg ccattttttt
     3601 cataaaaggt cctttactca tccgtaaata ctattttcct tctcaggaat gttaaacttt
     3661 gatgagctgt tataatttta tttaatcttc ttttttggtt tcctcttgcg ttgctaaagg
     3721 ttcagatttc ttttcttctg tttcgtctgt aacaggtatc actattgtct cggattcttc
     3781 tctttctttt tcagtttttt ccgattctct ttcctcttca gaagtttctt ttataatagt
     3841 tatttcttcc ggttctatat gttcgggttc aggaattaca ggttccacag gaatttcttc
     3901 tgccgctttt tcttcttccg cttcaatttc cacaggaatt tcttctgttt ctatcttttc
     3961 ttcttcagtt tcaatttcca cgggtatttc ttctg
//
LOCUS       DAOOZV010000099         3770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_19730_length_3770_cov_67.596501, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000099 DAOOZV010000000
VERSION     DAOOZV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19730_length_3770_cov_67.596501"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLW08_07440"
     CDS             <1..213
                     /locus_tag="PLW08_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52468.1"
                     /translation="HSYPSDMIPISFPYRDMGKIRDLIDLYSVKEKEKRAEKSRKLYL
                     MPSRLPEKEKTNRMKCSLLLVITYYK"
     gene            317..1516
                     /locus_tag="PLW08_07445"
     CDS             317..1516
                     /locus_tag="PLW08_07445"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPL52469.1"
                     /translation="MRILVLNCGSSSIKYQLIDMETESLMAEGIVEKIGEDIALFTYK
                     SEKYTKKKREMVVDNHEQGLQLILEALIDPANGVLNGLDEIDAVGHRLVHAGEHYSDA
                     VKVTEHVIQVMQECVSLAPLHNPANLKGIEAVKVNMPNCPQCGVFDTAFHQTMPAMAY
                     LYPLPLELYTNYKIRRYGFHGTSHKYVSLKAAEFLQRPLESLKIITCHLGNGASMTAI
                     KNGKSIDTSMGFTPLEGLMMGTRCGDIDPAIPIHLQQQLGLSVDEVNNILNKKSGMLG
                     LSHISNDMREIENEVLVKNNPKAIMALDVYCYRIKKYIGSYFAVLNGLDVLVFTGGVG
                     ENMPILREQVCRDLEAIGIKLNLEENSQRTDGIQVLSANDSSVTVLKIPTNEELMIAR
                     ETKRLLS"
     gene            1590..1850
                     /locus_tag="PLW08_07450"
     CDS             1590..1850
                     /locus_tag="PLW08_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPL52470.1"
                     /translation="MRIDVLLHKLCLTKTRSIAKNACDKNLVLINGKPAKASADVKAG
                     DIIIFRLYNAEHEIRITQIPEGNVAKKDATRYYELLRKEDLS"
     gene            1942..2847
                     /locus_tag="PLW08_07455"
     CDS             1942..2847
                     /locus_tag="PLW08_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52471.1"
                     /translation="MKRHFLLFILLCLSRFLFSSYPLQLKTNNLAIYAQNPVSQPLAE
                     MLKAMDENIDSFQMELGVYVDALAPVYLIENNRSYQTLALGKAKIVEFSDAFYSGTEK
                     RIYVKPLASIQENHRKILMHEYIHWYLEQVFTQTPLWFHEGMATHFSRQMGFEQYLYF
                     LQQSFLGEKSDLFRLSYSYPEKKEDWSLFYLSSTMAISYLKNKKNEQWNSFWEMVAQQ
                     HRKNLQAPFTDCFNRAFHTTFYDFHKEYARYIKHLRYQYLFWSINALLALLIPVILII
                     AWRIRKKRLASLPDLPLPEDEETEM"
     gene            3024..3698
                     /locus_tag="PLW08_07460"
     CDS             3024..3698
                     /locus_tag="PLW08_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HPL52472.1"
                     /translation="METMQNHPYKPFIPKDAKVLLLGSAPPYRFCTGNANDLKNKDMD
                     YYYGSNSNLLWEILFTAFEPDKIDSISYLRSLEEDRKIKTAYQVDFLQSFLARNQLGM
                     ADILLKFSRKGTSAEDYKLTVLEYQDILGILYNNSSLTKILCTSKNRVHYWLMDYLKQ
                     QKHYDLIEIRDNGNSLILKNNPHREIKIGILPSPSARSVMRYANKTEFLQQVSQIYKQ
                     AITNDE"
BASE COUNT         1265 a          656 c          747 g         1102 t
ORIGIN      
        1 cactcatatc cttccgatat gattcccata tcgttcccat atcgcgatat gggaaagata
       61 agggatctta ttgatctata tagcgttaaa gaaaaggaaa aaagagcgga aaaaagtaga
      121 aaattatact taatgccctc tcgtttaccc gaaaaggaga aaacaaacag aatgaaatgt
      181 tcccttctat tggttataac ttactataaa taaaatgctt agcttataag gaagggaaaa
      241 ccgagaaaat actatttgac atatagctca taaacaggac tttgagaaaa aaaataaacg
      301 ggaaaaaaga ggaaaaatga gaattttagt tttgaattgc ggcagctctt ctataaagta
      361 tcaacttatt gatatggaaa cagagagctt aatggcggaa ggtatcgtag aaaaaatagg
      421 ggaagatatt gctctattca cctataaaag tgagaaatac accaaaaaga aacgggaaat
      481 ggtggtggat aatcacgaac agggcttgca actaattctt gaagccctga ttgatcctgc
      541 caatggtgtt ttaaacggtt tggatgaaat tgatgctgta gggcatcgtt tggtgcatgc
      601 cggagaacat tattcagatg cagtaaaagt taccgagcat gttattcaag tgatgcagga
      661 atgtgtttcc cttgctcctt tacacaatcc tgccaatttg aaagggattg aagccgtgaa
      721 agtaaatatg ccaaattgtc ctcaatgtgg tgtttttgat actgcttttc atcaaacaat
      781 gcctgctatg gcatatttgt atcctttgcc tttggagctt tatacaaact acaaaatccg
      841 gcgctacggt tttcatggca caagccacaa atatgtaagt ttgaaagcgg cagaattttt
      901 acagcgtcct ctggaatcct taaaaattat tacctgtcat ttgggaaatg gagcttcaat
      961 gactgccatc aaaaacggca aatccattga tacttctatg ggttttaccc cgctggaagg
     1021 tttaatgatg ggcactcgct gcggagatat tgaccctgct attccgattc atttacaaca
     1081 acaactggga ttaagtgtgg acgaagtaaa caatattttg aacaaaaaaa gcggaatgtt
     1141 gggtttaagt catatttcca atgatatgcg ggaaattgaa aatgaggttt tagtaaaaaa
     1201 caatcccaaa gcaattatgg cattggatgt ctattgttac cgcatcaaga aatacattgg
     1261 cagttatttt gccgtcttaa acggattgga tgttttggtt tttaccggtg gagttggtga
     1321 aaatatgcct attttgcgtg aacaggtttg ccgtgatctg gaagccatag gcataaaatt
     1381 aaatttggaa gaaaattccc aacggactga tggaattcaa gttttaagcg caaatgattc
     1441 ttcggttacg gttctaaaaa tacccactaa cgaagaattg atgattgcgc gggagacaaa
     1501 acgcttactt tcttgaaaaa taatgaattg ttaaaagggc ttttccttat ggaaagagct
     1561 ctcttgaact ttaagcagga aggataaata tgcgcattga tgtgcttttg cataaactct
     1621 gtctgacaaa aacccgttcc atagctaaaa atgcctgcga taaaaatctg gtgcttatta
     1681 acggaaaacc ggctaaggcc tctgcagatg taaaggcagg agatattata attttccgct
     1741 tgtataatgc ggaacacgaa attcggataa cccaaatccc ggaaggaaat gtagctaaaa
     1801 aggacgctac ccgctattac gaattattgc gtaaagaaga cctttcctga aaatatactt
     1861 ttaaaggata tcggagtgcc gaattccttg tgccaaaacg gaatttgctg ttcacagtta
     1921 aaaagaacaa ggaaccaaaa cttgaaaagg cattttttgc tgtttattct tttatgccta
     1981 tcacgatttc ttttttccag ctatccttta cagcttaaaa ccaataactt ggcgatttat
     2041 gctcaaaatc cagtttccca acctctggca gaaatgctga aagcaatgga tgagaatatt
     2101 gattcttttc agatggaact gggcgtttat gtggatgccc ttgctccggt ttatctgatt
     2161 gaaaataata gaagttatca aacattggct ttgggcaagg caaaaattgt ggaattcagc
     2221 gatgccttct atagcggaac cgaaaaaaga atttatgtaa aacccctggc ttctattcag
     2281 gaaaatcacc gcaaaattct aatgcatgaa tatattcact ggtatctgga acaggtcttt
     2341 acgcaaacac ctttgtggtt tcacgaagga atggcaactc atttttcccg gcaaatgggt
     2401 tttgaacagt atttatactt cctgcagcag agctttttag gggaaaaaag cgacctcttt
     2461 cggcttagtt acagctaccc ggaaaaaaaa gaggattggt ccctattcta tctatcttcc
     2521 acaatggcaa tcagctatct gaaaaataag aaaaatgaac agtggaattc cttttgggaa
     2581 atggtagctc aacagcatag aaaaaatctg caggcacctt ttaccgactg ctttaaccgc
     2641 gcttttcata ctacttttta tgactttcac aaggagtatg caagatatat taaacatttg
     2701 cgctaccagt atctgttttg gagtataaat gccctcctgg ctttattgat tcctgtgatt
     2761 ttaattattg cctggcgaat tagaaaaaaa cgacttgcct ctcttcctga tttacctttg
     2821 cccgaagatg aggaaacaga aatgtaaaca tagctcacct ttttgtgtag acagaactca
     2881 ctgtagctcc gcgttaattt aatttttttt aataatagag taactacaag gtttattgga
     2941 aatgggaaaa aacataagaa cagggttttc caaaaaagcg gaggaatagc gtcctccagc
     3001 tacacttttt aggagtttat gatatggaga caatgcaaaa tcatccctat aaacctttta
     3061 ttccaaaaga tgctaaagtt ctgcttttag gtagtgcacc tccctatcgt ttttgtaccg
     3121 gtaatgctaa tgacctgaaa aataaggata tggactacta ttacggtagt aacagtaatc
     3181 ttctttggga aatattattt accgcatttg aaccggataa aatagatagc atctcttact
     3241 tgaggtcatt ggaagaagac agaaaaatta aaacagctta tcaggttgat tttctacaga
     3301 gctttttagc ccgaaatcaa ctcggaatgg cagatatcct attaaagttt agcagaaaag
     3361 gaaccagcgc agaggactat aaattaacgg ttctggaata tcaggatatc ttaggaattc
     3421 tttataataa ttcttctctt acaaaaatct tatgcaccag caaaaataga gttcactatt
     3481 ggcttatgga ttatctgaaa cagcagaaac attatgattt aattgaaata agagataatg
     3541 gcaattccct tatcctgaaa aataatcccc accgggaaat aaaaataggg attttacctt
     3601 ctccttcagc tagaagtgtt atgcgttatg ctaataaaac tgaatttctg caacaagtat
     3661 cacagatata caagcaagcc attactaatg acgaatgaaa aatgaagaat gtgagggttt
     3721 agttgtcctg aaaaaaacgg actgaaatta tggagaaaat aggcattcag
//
LOCUS       DAOOZV010000100         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_21269_length_3544_cov_43.684723, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000100 DAOOZV010000000
VERSION     DAOOZV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21269_length_3544_cov_43.684723"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3544)
                     /locus_tag="PLW08_07465"
     CDS             complement(<1..>3544)
                     /locus_tag="PLW08_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPL52473.1"
                     /translation="LGFNIVGLPLVSMPNFVVRMKHTTADNVSSWINADNLVTVYSNP
                     NYLPTETGWNMYNFSIPFEWNGEDNLLVDTAFGVASSYNRSGTVQFTSIGNGYRFKRN
                     DDNDQTNVFSEGETSFQRPNIKLIVEQITIGPQISVNPSSLDFGGIPAGQTQTRQFTI
                     QNYGNETLSGTIITPEGFTVSDSLRINLLTADNNAFSRNTFSFTIPAGTSNTYILNFS
                     PLTAGSYNGNVIISSNAENNPLINLQVTGSAYLPPAIEVSTNSLTATLDAGTQTNQTF
                     TISNTGGLPLSYQISISEDSVYTQKITKADKSIAGSTLTLNYADYEPGATLDWTFTLY
                     NNSTDVEWLKDVYVTFPDGIIVNSATNFTGGTEDMLPDLTSGNGITIHWHGLTTSGWG
                     VVQGGQSATATVNVTILPVFYGDLILSYTIQGDVYGSEPHQIDGQIALSQILTPIPWF
                     SLEPMQGEVLPGENATITSYFSALTTQPGIYNALLNIASNDPFQPLTILPVCMNVLQP
                     NHPPVINLPENFSFNANESLLVDFTPYVSDPDNDPLTLTCSGNTNINVTITGLQVSLS
                     SLHNWYGTEIIFFTVSDGMFQTSDSLTVIVLLNITTGVIVDSDNLPTNWTVNNSPFNI
                     VEPIVITTDQNITLEPGIVVQIWNDEPINVLGSISANNVTFAPGFPDLLWGGLEITGT
                     TGNRTESNITNCEILNAVNAITVNNCSPVINTVYIAPIDTTALINGTGITISGISSPP
                     INNVTILSYHTCIQVVRDAEILPNEPFINNLVLRNSSVVPRQEEENTKGIVIEGNSNV
                     IISNAEIKDYNNAIKVENTNVQQSATPSLSNIRIRNTSSTLRNEDKCGIELKGNENAI
                     LYDVRIEECNKGIKIENTETGVSATPSLDFIRIRNTSSTLRQELENIGILVESNVIPV
                     LRDVQIEDAETGIQINAGGNLDLKYSLLLNCQTGLKNYSTELMPITRNSFVIEEDQVP
                     PDFLQNCVALSLNDILNFEFSNNTLYGYPKIAAVNSAHLNFINNIVWSNSALNNPFEC
                     VNGTYNVAYNDINYGSQVFPGTGNINANPNFVNALELDFALQYNSPCIDAGYPDAEPD
                     PDGTIADMGCYYYHHCAVFESPEGPFYVQQPLQFINTSIGHNTPESYATWFLNGIPVS
                     NDYNLNTIIDTWGNYDLQLVMTSGPLVDSSSVFQF"
BASE COUNT         1121 a          651 c          706 g         1066 t
ORIGIN      
        1 aaattgaaaa acactgctgc tatccacaag gggtccggaa gtcattacca gctgcaaatc
       61 atagttaccc caggtatcaa taatcgtgtt cagattatag tcgttggaaa caggtattcc
      121 attcaaaaac caggtagcat aactttcggg agtattatgt ccaatagaag tatttataaa
      181 ctgaagaggt tgttgaacat agaaaggacc ttccggcgat tcaaaaacgg cacaatgatg
      241 ataatagtag catcccatat cggcaatagt tccatcaggg tctggttccg catcaggata
      301 accggcatca atacagggac tgttatattg caaggcgaaa tcaagctcca aagcattcac
      361 aaagtttgga ttggcattga tattaccagt tccgggaaag acctgactgc catagttaat
      421 atcgttgtaa gcaacattat aagttccgtt tacgcattca aagggattat tcagggcaga
      481 attgctccag acaatattat tgataaaatt cagatgggca gaattaacag cagcgatttt
      541 aggataacca tagagggtat tattgctaaa ttcaaaatta agtatatcat taagagagag
      601 agctacacaa ttctgtaaaa aatcaggtgg cacttggtct tcttcaataa caaagctatt
      661 acgggttata ggcatcagtt ccgtactata gtttttcagc cctgtttggc aattcagaag
      721 aagggaatat ttcaggtcca gatttcctcc ggcgttaatc tgtatacctg tttccgcatc
      781 ttcaatttga acatcgcgca aaacaggtat tacattactc tcaaccaaaa ttccgatatt
      841 ctccaattcc tgccttaaag tgctgctggt attgcggata cgaataaaat ccaaggaagg
      901 agttgccgaa actcccgttt cagtattttc tattttaatt cctttattgc attcctcaat
      961 tctgacatca tataaaatag cattttcatt gccttttagt tcaattccac atttatcttc
     1021 atttcgtaaa gtactacttg tattacgaat gcggatattg cttagggaag gagttgcgga
     1081 ctgctgaaca ttggtatttt ccaccttgat agcgttgtta tagtccttta tttcggcatt
     1141 tgaaataatt acattgctat taccctcaat tactataccc ttcgtatttt cctcttcctg
     1201 gcgaggcacg acagaagaat ttcgtagcac taaattattg ataaatggct cattaggaag
     1261 gatttcggca tcccgaacaa cttgaataca ggtgtgatag cttaagatgg taacattatt
     1321 tatgggagga ctagaaatac ccgaaatagt aattcctgtg ccattaatta aagcagtggt
     1381 atctatgggg gctatataga cagtgttaat aacaggacta caattattta cggtaatagc
     1441 attgacagca ttcaggattt cacagttggt aatattgctt tctgttcggt ttcctgtcgt
     1501 tcccgtaatt tccaaaccac cccaaagcaa atccggaaag ccaggtgcaa aagtgacatt
     1561 attggcagaa atagaaccca gaacattgat aggttcatca ttccatattt gaacaactat
     1621 ccctggttcc agagttatat tctgatcggt agtaattact attggctcta caatattaaa
     1681 aggtgaatta ttaaccgtcc agttagtcgg taaattgtct gaatccacaa ttactccggt
     1741 ggtaatattc agcaaaacaa ttacggttaa agagtcgctg gtttgaaaca taccatcaga
     1801 aacagtaaaa aatataattt cagttccata ccagttatgc aaagagctca aactaacctg
     1861 caaaccggtg atggtaacat tgatattggt atttccacta caagttaagg ttaagggatc
     1921 gttatctgga tcgcttacat aaggagtaaa atccactaat aatgattcat tggcattgaa
     1981 agagaaattt tccggtaaat ttatcaccgg aggatgattg ggttgaagaa cattcataca
     2041 aacaggtagg attgtaagtg gttgaaaagg atcattggaa gcgatgttta agagggcatt
     2101 atatattccg ggttgggtag ttaaggcaga aaaatacgag gttatggtgg cattttcacc
     2161 tggaagaact tccccctgca ttggctcaag agaaaaccag ggaatgggag taaggatttg
     2221 actaagcgca atttgaccat ctatttgatg cggttcactg ccataaacat caccctgaat
     2281 agtgtaagac aggataaggt ctccataaaa gacaggaaga atcgttacat ttacagttgc
     2341 ggtggcagat tgtccaccct gaacaactcc ccagccactg gttgttagtc cgtgccagtg
     2401 aattgtgatg ccatttccgg aagttaaatc aggaagcata tcttcagttc cgcctgtaaa
     2461 attggtagca gaattgacaa taattccatc ggggaaagtt acatacacat ctttcagcca
     2521 ttcaacatca gtgctgttat tatagagagt gaatgtccaa tccaaagttg cacccggttc
     2581 ataatcagcg tagttcaatg ttaaggtgct gcctgcaata cttttatcag ctttggtaat
     2641 cttttgagta taaacgctat cttcgcttat tgagatttga taagataagg gtaaaccacc
     2701 cgtattggaa atagtgaagg tttgatttgt ctgggtgcct gcatccaaag ttgctgtcaa
     2761 tgaattggta ctcacttcta ttgccggagg aagatatgca ctgccggtaa cttgtaaatt
     2821 aataagagga ttgttttcag cgttactgga tataattaca tttccattat aacttcctgc
     2881 cgtaagggga gaaaaattta aaatataagt attggatgta ccggcaggga tagtaaaact
     2941 aaatgtattg cggctaaaag cattgttatc tgctgtcagt aaatttatcc ttaagctatc
     3001 cgaaacggta aaaccctcag gagttatgat tgttccgctt aaggtttcat taccataatt
     3061 ttggatggta aattgcctgg tttgggtttg tcctgcagga ataccgccaa aatcaagaga
     3121 acttgggttt actgaaattt gaggaccgat agtaatttgt tctacaataa gcttaatatt
     3181 gggacgttga aaagatgttt ctccttctga aaaaacattg gtctgatcgt tatcatcatt
     3241 tcttttgaaa cgataaccat ttcctattga agtaaattga acagtccctg aacgattata
     3301 actgcttgcc actccaaaag cagtatctac taaaaggtta tcctctccat tccattcaaa
     3361 aggaatggaa aaattataca tattccaacc ggtttcagta ggtaaataat tgggattaga
     3421 ataaaccgtc accaaattat ctgcattaat ccaggagctg acattatctg cagttgtatg
     3481 tttcatacgg acgacgaaat taggcattga aactaagggt aaaccaacta tattaaaacc
     3541 aagc
//
LOCUS       DAOOZV010000101         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_21536_length_3507_cov_54.951622, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000101 DAOOZV010000000
VERSION     DAOOZV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21536_length_3507_cov_54.951622"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..724)
                     /locus_tag="PLW08_07470"
     CDS             complement(32..724)
                     /locus_tag="PLW08_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL52474.1"
                     /translation="MHIAFKNVSINGLLHNFNLNFTTDGCTVLYDPCEKYSSTILTAL
                     MGLYNLDEGNILLDGISREEYCRQNSLISTFSLVFDEGIMLSNLSLCENLLLPWNKRF
                     ASEDQNAFEQELKLLMEELNLNCGWELRPAYLSPAERKFFCFIRSLLLKPKIMLIDDP
                     YYLFNKEERRLLLGFLQKHSKKRSGTEFSDYPKSENTEGIYLQEMLIGTSDDDFTGEI
                     ATQVIDLSEICY"
     gene            complement(724..1485)
                     /locus_tag="PLW08_07475"
     CDS             complement(724..1485)
                     /locus_tag="PLW08_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL52475.1"
                     /translation="MQHNRKKSLGDFFLFLEETVTELLHIRHNRHVSFLVVIRQILFT
                     GVEALPLIGFIALAIGCLTIMQGYAFLSNFGQGIWVHIILVRVVVGELSGIITALVVI
                     ARSGTAISTELGNMVVNREMQLLKSFDIAPMGYLVVSRILGVVISMLVLTLYFNIIAV
                     LGGWLFAQFFSHLAFGAFMNDFLSVLTLPDIFMIILKSVIFGFVIAITASYEGMSVYR
                     ASTEVPQRTIKAVVISIFSVLILDIIISWLFWIGA"
     gene            complement(1513..1869)
                     /locus_tag="PLW08_07480"
     CDS             complement(1513..1869)
                     /locus_tag="PLW08_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52476.1"
                     /translation="MKKTMLILALMAMLMLIATAGYAYKGSPVESFDITPNPMFKECV
                     ISLAVSVPLNINVQIQSCDGKVICDLYEGFVGKDIYLNWERTDNYGNYVPDGEYFVVV
                     NFSTRYTSLKKTLILK"
     gene            complement(1911..2828)
                     /locus_tag="PLW08_07485"
     CDS             complement(1911..2828)
                     /locus_tag="PLW08_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPL52477.1"
                     /translation="MNIFLGIDIGGSSFKYGWGNCEEGLQSYQTLVLQRKNIDDFFAV
                     AKELFNDVDSKIGLNNIRGIGIGIPGAIDKTTGLVTENNPNLPFWVEHHPRELLPENC
                     NIPFAFDNDANLMALAESYTQKKNYVIGVTVGSGIGCGIVIDGKIYHGAYGFAGELGH
                     ICIVDKGLRCNCGKNGCLEAYSSGEGLRRRLALKNPCYAEMDLPTILAIKETDNLVGD
                     YIKQGQLFLATALSGLATCLDPEIIIIGGGAMDLGLYYIEELEREIKKRLPLAHSIKL
                     KVAKAINGNKAGVLGAIKLIEDKFNSKEE"
     gene            complement(2945..3019)
                     /locus_tag="PLW08_07490"
     tRNA            complement(2945..3019)
                     /locus_tag="PLW08_07490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2985..2987),aa:His,seq:gtg)
     gene            complement(3062..3138)
                     /locus_tag="PLW08_07495"
     tRNA            complement(3062..3138)
                     /locus_tag="PLW08_07495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3102..3104),aa:Arg,seq:tct)
     gene            complement(3151..3227)
                     /locus_tag="PLW08_07500"
     tRNA            complement(3151..3227)
                     /locus_tag="PLW08_07500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3191..3193),aa:Met,seq:cat)
     gene            complement(3351..3426)
                     /locus_tag="PLW08_07505"
     tRNA            complement(3351..3426)
                     /locus_tag="PLW08_07505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3391..3393),aa:Met,seq:cat)
BASE COUNT         1149 a          804 c          546 g         1008 t
ORIGIN      
        1 aaccctctca acctcttcta tttagttctg ttcagtagca gatttcagat aaatcaataa
       61 cctgcgttgc tatttcgcct gtaaaatcat catcactggt gccaattaac atttcctgta
      121 aataaattcc ttccgtattt tcgcttttgg gatagtcgga aaattctgtt ccgcttcttt
      181 ttttactatg tttctgcaaa aaacccaaaa gcaatcttct ttcttcttta ttgaacaaat
      241 aatagggatc gtcaatcaac ataatcttcg gtttcaagag caaagaccga ataaaacaaa
      301 aaaacttgcg ttctgccggg cttaaatatg caggtcttaa ttcccatccg caattcaaat
      361 tcagctcttc catcaaaagt ttcagttctt gttcaaaagc attctggtcc tcgctggcaa
      421 accttttatt ccaaggcaga agtaaattct cacataggga aagattggaa agcattatcc
      481 cttcatcaaa cacgagggaa aaggtggaaa taagggaatt ttgcctacaa tattcctccc
      541 gcgaaatgcc atccagtaag atattacctt catccaaatt atacaatccc attaaagcag
      601 ttaaaatggt agatgaatac ttttcgcagg gatcataaag cacagtacaa ccatctgtgg
      661 taaaattaag attgaaattg tgtaataaac catttataga aacattctta aaggcaatat
      721 gcactatgca cctatccaaa atagccagga aataattata tccaaaataa gcacggaaaa
      781 aatagaaata accactgcct tgatagtgcg ttggggaact tccgttgatg ccctgtaaac
      841 actcatccct tcataagagg cagttatggc tattacaaag ccgaaaataa cggatttgag
      901 gatgatcata aaaatatcgg gtagggttaa aacggataaa aaatcattca taaaggcacc
      961 aaatgccaaa tgactaaaaa actgagcaaa aagccaaccg cctaaaacag caattatatt
     1021 gaaataaagg gttaatacca gcatagaaat tactactccc aaaatgcgcg aaacaacaag
     1081 ataacccatc ggcgcgatgt caaaggattt caacaattgc atttccctgt tcacaaccat
     1141 atttcccaac tcggtagaaa tcgctgttcc acttctggct attactacta aagcggttat
     1201 aattccactc aattctccaa ctacaaccct caccaaaatt atgtgcaccc aaatcccctg
     1261 cccgaaattg ctcaaaaaag cataaccctg cataatggtt aaacagccaa ttgccaaagc
     1321 aataaaaccg attaaaggca gcgcttccac tcctgtaaac aaaatttgcc gaataaccac
     1381 taaaaaactt acatgtctgt tatgtctgat atgcaataac tcggttactg tttcttctaa
     1441 aaacaaaaaa aagtccccaa gggacttttt tctattgtgt tgcataagtt acatcactgc
     1501 tctaaaagtt tgttatttca gaataagcgt cttctttaag gaagtatacc gggtagaaaa
     1561 attaactaca acaaaatatt cgccatcagg aacataattg ccatagttat cagtcctttc
     1621 ccaatttaaa taaatgtcct tacccacaaa accttcgtat agatcgcaaa taactttgcc
     1681 atcacagctc tgaatctgaa cattgatatt aagaggaaca gacacagcta aagaaattac
     1741 acattcctta aacataggat tgggagtaat atcaaaactc tcaacaggag aacccttata
     1801 agcataccca gctgtggcta tcaacattaa cattgccatc agcgccagaa tgagcatcgt
     1861 ttttttcatt ttattcaccc cttatttaat gcctaattaa aaagcaagtt tcattcctct
     1921 ttgctattaa acttatcttc tatcagtttt atcgctccta aaactcctgc tttattgccg
     1981 tttattgctt tagcaacctt taatttaata ctatgtgcca gcggtaatct ttttttaatt
     2041 tctctttcca gttcctctat ataatataat cccaaatcca tagctccgcc accaataatt
     2101 attatttccg gatcaagaca agttgcaaga ccggataatg cagtagctaa aaaaagttga
     2161 ccctgtttaa tgtaatcccc tactaaatta tcagtttctt ttatagcaag gatggtaggt
     2221 aaatccattt ctgcataaca aggatttttt aatgccaaac ggcggcgtaa accttctcct
     2281 gaagaatacg cttccagaca gccatttttc ccacaattac atcttaatcc tttatccaca
     2341 atgcaaatat gacccaattc tccggcaaaa ccgtaagccc cgtgataaat tttgccgtca
     2401 ataactattc cgcaaccgat tccactgccg acagtaacac caataacata attctttttt
     2461 tgcgtatacg actcggctaa agccatcaaa ttggcatcgt tatcaaaagc gaaaggaata
     2521 ttacaatttt cgggaagcaa ttcacgcgga tggtgctcca cccaaaaagg caaattggga
     2581 ttgttttctg ttaccaaacc cgtggtttta tctattgcac caggtattcc aattccgatt
     2641 cctcttatat tgttaagccc gattttactg tcaacatcgt taaacaactc tttcgccaca
     2701 gcaaagaagt catctatgtt tttcctttgc agaacaaggg tttgatagga ttgtaaacct
     2761 tcttcacagt tgccccagcc atacttaaaa ctggaacctc caatatctat gcctaaaaat
     2821 atattcatcg gtcaccttat tacttcatct cttaaatgaa aaatcacttt ggtattcctt
     2881 tttgctattc ccgcgcaacc aggaatccat tcctttttgc cattcctgtc aaagaaagaa
     2941 taaatggggt gggtgatggg atttgaaccc acaacgcccg gaaccacaat ccggtgctct
     3001 gccgttgaac tacacccacc acaataatta tctctccctt aacagaaagt tgaatagcta
     3061 atggcacgcc agaagggatt cgaacccctg acccgcagct tagaaggctg ctgctctatc
     3121 ctactgagct actggcgcct gtgtgaaatc tggtagcggc gcagggattt gaaccccgga
     3181 cctgcggatt atgattccga cgctctaacc agctgagcta caccgccact tttcttttat
     3241 aagatagtcc actttatata ggggtgacaa attcaatttt tcgtcactta tttaaaactc
     3301 ctattcctaa aaaaaagagt tcgtcttcta aaagccaaac tctaaaaaat tggtagcgag
     3361 ggaaggattc gaaccttcgg ccttcgggtt atgagcccga cgagctacca gctgctccac
     3421 ctcgcgttat caatttatcc ttaaaaaata aaaccccatt tctgtcaagc aaaaaattac
     3481 ggaatagcga aaaggtgaga aatcaaa
//
LOCUS       DAOOZV010000102         3500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_21600_length_3500_cov_67.890856, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000102 DAOOZV010000000
VERSION     DAOOZV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21600_length_3500_cov_67.890856"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..327)
                     /locus_tag="PLW08_07510"
     tRNA            complement(250..327)
                     /locus_tag="PLW08_07510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Pro,seq:ggg)
     gene            478..2388
                     /locus_tag="PLW08_07515"
     CDS             478..2388
                     /locus_tag="PLW08_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPL52478.1"
                     /translation="MSLIQVIDAGLEFAGNYVLRGINCTLEYNSRIGLIGSNGSGKTT
                     LIKLMLGIIPPSEGKVLRAKKCKIAYLPQNPVLEKDILMGNYIQKSRPDLLSLQQQID
                     ELSSLLQKKHTERTENELKIALDKFQASGGDEFANSMKYVITSLGFSEDDYEKPLYSF
                     SGGEQTRLCLASILLMPYDLLILDEPTNHLDIAMISWLEKYLNNSKAPFLLVSHDRIF
                     LDNTVSTIFQLENTQLSITKGNYSSFAQAKAIEQKTQERQFEKQKKFIAETEDFISKN
                     IAGQKTNQAKSRLKMLSRIEIIQKPQQAKKIKLNIQTAGRSGNDVYTLKDVEFGIPEG
                     PLLAKNVNLQVHYRDRVCIIGPNGCGKTTLLKILLEEREIFSGSLKIGASLEVGYYDQ
                     HQVFLDEDLTVMDTLWQIVPSETRGYVLSYLARFGFQGLDVEKKVEILSGGEKSRLML
                     CVLIHQNPNLLIMDEPTNHLDMEMSDSLLSALQNYRGTIIFVSHDRYFIRQLATKYWV
                     FHKALENNILYPTISEMETNLEEALELAFTIPEPPKAKVNIRERKKKINPYYLEQIHK
                     QIENEQTSITKNHSQIEKINSQLALPSTYSNAMRAKELRQQIDLLLNEIENSKKHIAE
                     LETQYLELSYDS"
     gene            2378..3367
                     /locus_tag="PLW08_07520"
     CDS             2378..3367
                     /locus_tag="PLW08_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPL52479.1"
                     /translation="MIPKRIGFTTSFPVEVIFAAGHIPVDLNNVFITSDSSVKVQKAE
                     LKGFPRTLCSWIKGNYIAALSSQLDLIIGIVEGDCSNSNSLLDMFTEEHLPVYRFSFP
                     ADKNYADLNKEISRLEDYFGVSRKETLKAKKRLDEIRRKLIILDEWTWKEHRVSGLEN
                     HFWLVNSSDFMGNPDRYESELDAFLAVAEKRDPLPAKLRLAYLGVPPIYKNIYETIIE
                     LGGDVLFNEVQRQFSMPYLLTDIVQQYLAYTYPYSVFDRLQDIKPELEKRQIEAVISY
                     TQSFCHLQLDNLLLRKHIDLPFLTLEGDQPEEIDARTLLRLESFFEVHSKIDK"
BASE COUNT         1169 a          620 c          709 g         1002 t
ORIGIN      
        1 atcagcagga tttcacatat cgtgcagatt gaaaatgcat aaatattttt gtagcttatg
       61 tggattccta ctttcgcagt aatgaaaaac ggcttatcat tcaaaaaatg atattactta
      121 atggtaaata aaagaaaact ccctgtaaat ccttttaatc ctgttcatcc cccacctatt
      181 caaatctgcg tcatctgcga aatctgcgtg aaaccttttt ctgtgtaatc tgtgaggaac
      241 aaaaaaaact ggtcgggatg acaggatttg aacctgcgac cacttgaacc ccattcaagt
      301 acgctagccg gactgcgcta catcccgaac cgtttaattt accttaaaaa atagggacgg
      361 gattttgaca agcaaaaaat gaggttgaca gcagaagtgt tttcctaaaa tagctttaag
      421 caagctggaa tgctgtcttc cagcaagaaa aaaatattat gaaagggata gaaagatttg
      481 agcttaatcc aggttattga tgccggactt gaatttgccg gtaattatgt tctaagggga
      541 attaattgca ctttagaata taacagcaga ataggactta tcggttccaa cggcagtgga
      601 aaaacgacac ttatcaaatt gatgttaggc ataattcccc caagcgaagg caaagttttg
      661 cgtgctaaaa aatgtaagat tgcctatctg cctcaaaatc ctgttttgga aaaggacatc
      721 ctgatgggta actatatcca aaaatcccgt cctgatctac taagtttaca acagcaaata
      781 gatgaactct catcattact gcaaaaaaaa catactgagc gcacggaaaa tgagctaaaa
      841 atagctttgg ataagttcca agcaagcggt ggtgatgaat ttgccaattc aatgaaatat
      901 gtaattacct cgcttggttt tagtgaagat gactatgaaa aacccttata ttcttttagc
      961 ggaggagaac aaacacgctt gtgccttgct tctatcctgc ttatgcctta tgatttattg
     1021 attttggacg aacccacaaa tcatttggat atagctatga tttcctggct ggaaaaatat
     1081 ttaaacaaca gcaaagctcc ttttttgctc gtttcgcatg atcgcatttt tctggataat
     1141 acggtttcca ctatttttca gctggaaaat acgcaattaa gcataactaa agggaattat
     1201 tcttcttttg cgcaagccaa agcaatagag cagaaaacgc aggaaaggca gtttgaaaaa
     1261 cagaagaaat tcattgccga aaccgaggat tttatcagta aaaatattgc cggtcagaaa
     1321 accaatcagg caaaatcccg tttaaaaatg ctttcccgca tagaaataat tcaaaaacca
     1381 cagcaggcaa agaaaattaa gctcaatatt caaacagcag gaagaagcgg aaatgatgtt
     1441 tataccttga aagatgtaga atttggtatc ccggaaggac ctcttttagc caaaaatgta
     1501 aatctgcaag ttcactatcg tgatagggtt tgtattattg gtcccaatgg ctgtggcaaa
     1561 acaactttac taaaaatctt gctggaagaa cgggagatat tttccggttc tcttaaaatt
     1621 ggagcttcct tagaagtcgg ctattacgat cagcatcagg tttttctgga tgaagattta
     1681 accgtgatgg atacattatg gcagatagta ccaagcgaga caagaggtta tgtgttaagt
     1741 tatttggctc gttttggttt ccagggtttg gatgtggaaa agaaagttga aatccttagc
     1801 ggaggtgaaa aatcgcgttt aatgctctgt gttttaattc atcaaaaccc taatctgctg
     1861 attatggatg aaccgacaaa tcatttggat atggaaatga gtgacagttt gctttctgct
     1921 ttgcaaaact atcggggaac cattattttt gtaagtcatg atcgctattt tattcgtcag
     1981 ctggctacaa aatattgggt gtttcataaa gcgttggaaa ataatatact ttacccaact
     2041 atcagcgaaa tggaaacaaa cctggaagag gcattagagc tggcttttac aattcccgaa
     2101 ccgccaaaag caaaagtaaa tatacgggaa agaaagaaga aaatcaatcc ctattatctg
     2161 gaacaaattc ataagcaaat tgaaaatgag cagacatcaa ttacaaaaaa tcatagccaa
     2221 attgaaaaaa taaattccca gctggcactt ccttctacat acagtaatgc tatgcgggca
     2281 aaagaattac gacaacaaat agacctgctg cttaacgaaa ttgaaaacag caaaaaacac
     2341 attgccgaac tggagaccca atatctggag ctgagctatg attcctaaac gaattggttt
     2401 tactacttct tttccggtgg aagttatttt tgcggcagga catattcctg ttgatttaaa
     2461 taatgttttt atcaccagtg attcttcggt aaaagtgcaa aaagccgaac tgaaaggttt
     2521 tcccagaacc ctttgcagct ggattaaagg aaactatata gcggctcttt cttctcagtt
     2581 ggatttgatt atcggtattg ttgaagggga ttgttccaac agcaattcac ttttggatat
     2641 gtttaccgaa gagcatcttc cggtataccg tttttctttt ccggcagata aaaattatgc
     2701 agacctgaat aaggaaattt cccggctgga agattatttc ggtgtttccc gcaaagagac
     2761 attaaaagca aaaaaacgcc tggatgaaat tcgccgtaag cttattatct tggacgaatg
     2821 gacttggaaa gaacaccggg ttagtggact ggagaatcat ttttggctgg ttaattcttc
     2881 tgattttatg ggtaatcccg accgctatga aagcgaattg gatgcatttt tagcagtagc
     2941 agaaaaacga gacccattac ctgcaaaact gcgtttagca tatttaggag tgcctccaat
     3001 ctataaaaat atttacgaaa cgattataga attgggtggg gatgtccttt ttaacgaagt
     3061 gcaacgccaa ttttctatgc catatctgct aacggatata gttcaacaat acctggctta
     3121 tacttatccc tacagcgttt ttgaccgttt gcaggatatt aaaccagaac tggaaaaacg
     3181 ccaaatagaa gcagtaatta gttacacaca aagcttttgc catctgcaac tggataactt
     3241 gctcttaaga aaacatatag acctaccttt cttaactttg gaaggtgacc aacctgaaga
     3301 aatagatgcc agaactttgc tgcgactgga aagctttttt gaagtgcata gtaaaattga
     3361 taaataggta ttggatgaac aggatgatta gaatttaaag gtttttgaga gataatggag
     3421 ggttgagagg gtttagaagg tttagagggt tgagaaggtt gagattcaaa aaagcccgta
     3481 ggacgacaca aatatagcga
//
LOCUS       DAOOZV010000103         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_22694_length_3353_cov_59.840509, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000103 DAOOZV010000000
VERSION     DAOOZV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22694_length_3353_cov_59.840509"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..606
                     /locus_tag="PLW08_07525"
     CDS             217..606
                     /locus_tag="PLW08_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52480.1"
                     /translation="MQKYQIVIILITALLFSACCHHKTKEVTPETENFNYTGSKFHNN
                     YIWGGAMNLAWNELIESFTSEPIELDTKDESTLKTLYCLNNPVFSKADIDEASYYVRS
                     GYGQKQLILSIMNARQNFLIKLYQICS"
     gene            597..1265
                     /locus_tag="PLW08_07530"
     CDS             597..1265
                     /locus_tag="PLW08_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52481.1"
                     /translation="MQLKLGDRDIISYAYFLKQIEYAIAFQKQELNFMDQKVNGFTSG
                     AESYSNVYILAYENDDKFLLALKLKDNQDQIFLAKGFPMDKPDEVLKMLKTKAPAQEK
                     ADFMPGTVMNRKDIFQAPILHLNYERQYKEMLNKKVTNKKLEGYQIALMQEIIKFDMD
                     EKGARVENEAIIGMITSINPDAYKPKNLILDKPYWVIMKRANSNNPYFILGVGDTKLM
                     HASD"
     gene            1525..2691
                     /locus_tag="PLW08_07535"
     CDS             1525..2691
                     /locus_tag="PLW08_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPL52482.1"
                     /translation="MKDLIINAFESALRQLNTLPELKKDLEKRPLSPPVKILAIGKSA
                     FPMAKVCVDILNKREINYSGYLLTKYGNVAGIIPNLIVREASHPIPDINTIKYSREIL
                     SWLQELYANEEIIILLSGGGSSLFEVPIKGFTLEDLIAFNKNLLQSGLNIKEMNYERA
                     KKSKVKAGKALNYIKAMNIYCYALSDVPDNEPEIIASGCFFPANSQKIEDNYYQAKLK
                     KGKQKLYYSIIGDNFSLRYKLAKELPPPVYVHPRYFSENVENVAEFIADFAFSTDKKG
                     MYIFGGEAPVKVAGNGIGGRCTHLALLLAKKIAGKKHITFYALASDGNDNLENVSGAC
                     VNQNTLNQLQAKGIDISSVIAECDSYSALKTINAVVPVFQNPLNLNDIYLLQLY"
     gene            complement(2726..2992)
                     /locus_tag="PLW08_07540"
     CDS             complement(2726..2992)
                     /locus_tag="PLW08_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPL52483.1"
                     /translation="MDSYFRRNDKWLFFQEIIFNIFWTYLVNEQVVIELKSVEVLLPV
                     RHKQLITYLKLAKKPLGFLINFNTHSIKDNIIRIANDKNHPDLK"
     gene            complement(3022..3219)
                     /locus_tag="PLW08_07545"
     CDS             complement(3022..3219)
                     /locus_tag="PLW08_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPL52484.1"
                     /translation="MNEISYQIRSAVFDVYNELDPGLMENVYEKALIIELLNRGLHTE
                     NQVPIEVLYKGLDLGLILYLS"
BASE COUNT         1147 a          574 c          568 g         1064 t
ORIGIN      
        1 tgaaaaattg agaatggaaa attataaatc ctttctaata tatatctgtg ttatctgtgt
       61 aatctgtgag agaaaaaaat tacacaaatc ctgctcctcc tctgcccatt aacaattgaa
      121 atctatatga tatgcataat tcgttaaaga tatacattgt tattgacaca atttccccgt
      181 aatttatcct gccaaaaaga tgaaatgagg ttaaaaatgc agaaatatca aattgtcata
      241 atcctgataa ctgctctctt attttcggct tgctgccacc ataaaaccaa agaggtcact
      301 ccggaaactg aaaactttaa ttataccggc agtaaatttc acaacaatta tatttggggc
      361 ggagcaatga atcttgcatg gaatgaactg atagaaagtt ttacaagtga acccattgaa
      421 cttgatacta aagatgaatc cacacttaaa acattgtatt gcttaaataa tcccgttttt
      481 tctaaagccg atatagatga ggcaagttat tatgtgagaa gtggatacgg tcaaaaacag
      541 ttaatcttat caattatgaa tgcaaggcaa aatttcctga taaaactata ccagatttgc
      601 agttaaaatt aggtgacaga gatattatct cttatgccta tttccttaaa caaattgaat
      661 atgcaatcgc tttccaaaag caggaattga attttatgga tcaaaaagtt aatggtttta
      721 cttccggtgc tgaaagctat tcaaatgttt atattttggc ttatgaaaat gacgataagt
      781 tcttgctcgc tttgaaatta aaagataacc aagaccagat ttttctggca aagggatttc
      841 caatggataa acccgatgag gtcttaaaaa tgctgaaaac aaaggcacca gctcaagaaa
      901 aagcggattt tatgcccgga actgtaatga accggaaaga tatttttcag gcaccgattt
      961 tacacctaaa ttatgaacgc cagtataaag aaatgctaaa taaaaaggtt acaaacaaaa
     1021 aacttgaggg ttaccaaatt gctctgatgc aagaaattat caaatttgat atggacgaaa
     1081 aaggtgcccg cgttgaaaat gaagctatca tcggaatgat aacttccata aatcctgatg
     1141 cttataaacc caaaaatctg atcttagata agccctattg ggtgattatg aaacgggcta
     1201 attccaataa tccctatttt attctgggag ttggtgatac aaaattgatg cacgcatctg
     1261 attgagagaa ttttaagcta aatcctattt aaccattaga gtgaaaattt ctcttttgtc
     1321 aaacttgata aagtccgctg gaatataagc ttatgtttga tactgcgtaa atatttttaa
     1381 gcttatacag atgtctgctt tcgcaggaat aacaaaggac ttttcattcc agaaataaaa
     1441 taattgcttt tctgttttgc cgaagcgaaa ttcggcaacc taattattct gccaaattgg
     1501 aatttggcaa cgcggaatta aaatatgaaa gacctaatta taaacgcatt tgaaagcgct
     1561 ctcaggcaac taaatacact gcctgaacta aaaaaggact tggaaaaaag acctctttcc
     1621 cctcctgtaa aaattttagc catcggaaaa agtgcttttc caatggctaa ggtgtgtgta
     1681 gatatattaa ataaaaggga aataaattat tcgggctatc tgcttacaaa atatggaaat
     1741 gtagccggaa taatacccaa tttaatagtt agagaagcaa gccatcccat tcctgatatc
     1801 aatactatca aatatagccg ggagattctt tcctggctgc aggaacttta tgcaaatgaa
     1861 gaaattataa tcctgcttag tggaggagga agttctctat ttgaagtgcc aattaaaggt
     1921 tttactttag aggatttgat tgcttttaat aagaacctgc tgcaaagtgg cttaaatatc
     1981 aaagaaatga attatgaacg cgctaaaaag tccaaagtca aagccggaaa ggcacttaac
     2041 tatataaaag ctatgaatat atactgttat gctctttccg atgtcccgga taatgaacct
     2101 gaaattattg cttccggctg ttttttccct gctaattccc aaaaaattga agataattat
     2161 tatcaggcga aattgaaaaa gggcaagcag aaactttact attctattat aggcgataac
     2221 ttctctcttc gctataagtt agctaaagaa cttcctcctc cggtttatgt tcacccccga
     2281 tatttttctg aaaatgtaga aaatgtagcg gaatttattg ctgattttgc tttttccaca
     2341 gacaaaaaag gaatgtatat ctttggtgga gaagcacctg tgaaagttgc cggaaatgga
     2401 attggaggac gctgcacaca tcttgctctt ttgctggcaa aaaaaatcgc cggcaaaaag
     2461 catattactt tttatgctct tgccagcgat ggaaatgata atttggaaaa tgtatcaggt
     2521 gcctgcgtta atcaaaatac acttaaccaa ctgcaagcaa agggaataga tatctcttca
     2581 gttattgccg aatgtgattc ttattcagcc ctgaaaacta tcaatgctgt cgttcccgtt
     2641 tttcaaaatc cccttaattt aaatgatatc tacctcctgc aattgtatta atctcacgct
     2701 gattacgctg atttcgcaga ttttattatt taaggtctgg atgatttttg tcattggcta
     2761 ttcttatgat attgtctttg atggaatgag tattgaagtt aattaaaaag cccagaggtt
     2821 tctttgctaa tttcaagtat gttattagct gcttatgacg aacaggtaat aatacttcta
     2881 ccgatttcaa ttctattacc acttgttcat ttaccaaata agtccagaag atattgaata
     2941 ttatttcttg aaagaaaagc cacttgtcat tcctgcgaaa gtaggaatcc atataagttt
     3001 agtaatgtat ttatgtcttt ttcaagataa atatagtata agaccaagat caagaccttt
     3061 ataaagaact tctataggaa cttggttttc agtatgcaaa cccctattga gtaattcaat
     3121 aataagagct ttttcgtaaa cattttccat tagcccaggg tccagttcat tatatacatc
     3181 aaaaacagca ctcctaattt gatagcttat ctcgttcaaa tttattatat tataaatctt
     3241 taatctgcgt tatctgcgaa atctgcgtga aatattattg cagtattgtc atttttctaa
     3301 tgtaattcct ttccccacat cgcattatga cagtataaat ccctgaacta cat
//
LOCUS       DAOOZV010000104         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_23549_length_3254_cov_42.532041, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000104 DAOOZV010000000
VERSION     DAOOZV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23549_length_3254_cov_42.532041"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..3170
                     /locus_tag="PLW08_07550"
     CDS             147..3170
                     /locus_tag="PLW08_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPL52485.1"
                     /translation="MQKKFFVFLVALVWSIALVGASVTLDSLEISVQGGGYPRQGSTI
                     VITMYYRVADIPEGESIYVYGIATGAVDQAQIKWYPSSNGTGTASNQNVSVNQVANTI
                     PYDEDGNGYQSSHTATFTLPAPPSSSYKSFKINGKVYAATSDDEFTYFYSGIKLSKNY
                     LTINNPPVASSVVIVDPTLARVGQTLTGDYEISDPDGDEIDTPIFRWLSSTIETGTYT
                     AISGATGNTYTVQSADANKYIKFEVTPIAKTGELQGAPKLSDATPKVTGAPIIEIDPS
                     TLNIYEASANNGSLGDAYVKITCTNTSFKSSITNSAITISKDLDGLSKGSVTWISANE
                     IRLGFSGAAVNHEYAAKIDTIRITINKDQLEGVENNLTTQNGFNINFTNNPPTNLALD
                     SVGNNYVKITWDKPAGLLTSGSGLDYFNILRNETNIGSIYLDKGKGTYYYTDNTVTNG
                     TQYRYKVQAVYTAGTNETSTELRGTPLAITAFSFSTPPATGTIDHTNKTIKVVVPNGT
                     DRNALIANFTAPGAIVKVGTATQTSGSTSNNFTNPVTYTLTTNNDASTCSYVVTVNEK
                     LATPSPSVVGSNITTSSIKIHWDTITGADRYGVDVSENNSFPPGSFQHKEVSANFCIM
                     NGLKAGTKYYYRVQAIAEDQYFNSDYSSVYDTTTDALIPGAGSTEIDSSYETTINVGE
                     YVYGQETVTPTVKVHPAEFSPSENDIITVTMSYGTTPEGLQYNLAFENPTIGNGTFIL
                     SYSGLSYDPTDVGYRLNGGALHSVGGDGINTTDKIVIFTMSGLGKGSKAAYELQIVLN
                     DESGQTLPVVLTSFTATQMVDGKVRVDWVTQSETNLSGFRVLRNTVAEISTAMAVSPL
                     IEATNTSATVVYSFMDSEVPGNGIYYYWLQIEDLDGGISYSAVYTVQVTTGGVTNPII
                     PKITALLNPFPNPFNPDVTIPFDLAVDGNVTLKIYNLKGQLIKNLLNENCKANSYRIL
                     WDGKDNEGHTVSTGTYIIRMNAPNYQASHKISLIK"
BASE COUNT         1037 a          569 c          612 g         1036 t
ORIGIN      
        1 ttagtatata ttttaggtaa agtagagtag tagaatgaaa aagcgtaaaa ttatactaag
       61 ttcagtaaac acactctgtt ataagagaga ggcaatgttc tgtttctcgg ttttaataag
      121 aaagtcactt tatcagggag gataaaatgc aaaagaaatt ttttgttttt ttagtcgctt
      181 tagtttggtc aattgcttta gtaggtgctt ctgttacttt ggattcatta gaaatatctg
      241 ttcaaggtgg aggttacccc agacaaggat ctacaattgt tataactatg tattatcgtg
      301 ttgctgatat accagaaggg gaaagtattt atgtttatgg tatagctaca ggtgctgttg
      361 accaagcaca aataaaatgg tatccaagtt ctaatggtac tggaactgca tccaatcaaa
      421 atgtatctgt taatcaagta gctaacacaa ttccttatga tgaggatgga aatggatatc
      481 agagttctca tacagctact tttactttac cagcaccacc ttcaagttct tataagagtt
      541 ttaaaattaa cgggaaagta tacgcggcaa cttctgacga tgaatttaca tatttctatt
      601 ccggaataaa gctatcaaaa aattatttaa caataaacaa cccacctgtg gcttcaagtg
      661 ttgttattgt tgatcccact ttagccagag tagggcaaac tttaactgga gattatgaga
      721 ttagtgatcc tgatggtgat gaaatagata cccctatctt tcggtggtta agttctacta
      781 tagaaactgg aacttatact gctatttccg gtgcaacagg aaatacatat acagttcaat
      841 ctgcagatgc aaataaatat ataaaatttg aggtaacacc tattgctaaa accggtgagc
      901 tgcaaggagc acccaaatta agtgatgcta ctccaaaagt tacaggagct ccaattatag
      961 aaattgatcc cagtacactt aatatatatg aagcaagtgc taataatggt tccctgggtg
     1021 atgcctatgt aaagattact tgtaccaata ccagttttaa atcttccatt actaattccg
     1081 ctattactat atcaaaagat ttagacggat tatcaaaagg cagcgtaacc tggattagtg
     1141 ccaatgagat tcgtcttggt ttttcaggtg cagcagtaaa tcatgaatat gctgccaaaa
     1201 tagatactat ccgtattact ataaacaaag atcaattaga aggtgtagaa aataacttaa
     1261 caacccagaa tggttttaac ataaacttta ccaataatcc ccctaccaat ttagctttag
     1321 atagcgttgg caataactat gttaaaataa cctgggataa acctgctggt ctattaacga
     1381 gtggaagtgg attggattat ttcaatattt taagaaatga aactaatatt ggcagcatct
     1441 atcttgataa aggtaaagga acttattatt atactgataa tactgttacc aatggaactc
     1501 agtatcgtta taaagttcaa gctgtttata ctgcagggac aaatgaaact tctacagaac
     1561 taaggggaac tcctcttgcg attactgctt tttctttcag cactcctcct gcaaccggaa
     1621 caattgatca taccaataag accatcaaag tagttgtccc caatggaact gatagaaatg
     1681 ctttaatagc taattttaca gctcccgggg caattgtaaa ggttggaacc gctactcaga
     1741 ccagtggctc aacttcgaat aatttcacta atcctgtaac ctatacttta actaccaata
     1801 atgatgcttc tacttgttct tatgtagtaa cggtaaatga gaaattagct accccctctc
     1861 cttctgtggt aggatcaaat ataaccactt ccagtattaa aattcattgg gatacaatta
     1921 caggggcaga tagatatggc gtagatgttt cggaaaataa cagtttccca ccaggcagtt
     1981 ttcagcataa agaggtatct gctaatttct gcattatgaa tggtctgaaa gcaggtacca
     2041 aatattatta ccgggttcag gcaattgctg aggatcaata ttttaacagt gattattctt
     2101 ctgtctatga tactacaact gatgcattaa ttccgggggc aggcagcacg gaaatagata
     2161 gcagttatga aaccacaata aatgtaggag aatatgttta cgggcaagaa acagtaactc
     2221 ctactgtgaa agttcatccc gctgaattta gtccttctga aaatgatatc attacggtaa
     2281 ctatgagtta tggaactact cctgagggtt tgcagtataa ccttgctttt gagaatccta
     2341 ctattggtaa tggaactttt attctttcct acagcggatt gagctatgat cccacagatg
     2401 taggttatcg tttgaatggt ggtgccttac attcggtagg aggtgatggt ataaatacaa
     2461 cagataaaat cgttattttc acaatgagtg gcttgggtaa aggaagtaaa gcagcttatg
     2521 aattacaaat tgttctcaat gatgaaagtg gtcagacatt acctgttgtt cttacatctt
     2581 ttaccgctac tcaaatggtt gacggtaaag tgcgtgtgga ttgggtaact caatcggaaa
     2641 ccaatctttc aggatttcgt gttttgcgca atacagttgc cgaaatttcc acggctatgg
     2701 ccgtaagtcc tctaattgaa gcaacgaata cttcagctac tgtcgtttat tcgtttatgg
     2761 acagcgaagt tccgggtaat ggcatttact attactggtt acaaattgaa gatttggatg
     2821 gaggtatatc ctattctgct gtctatactg ttcaagtgac tactggtgga gttacaaatc
     2881 ccataatacc aaaaattact gctttgctta atccttttcc caatcctttc aatcctgatg
     2941 taactattcc ttttgatctt gctgtagatg gcaatgttac cttaaaaatt tacaatttga
     3001 aagggcaatt gattaaaaac cttttaaatg aaaattgcaa agcaaattct tatcgtatcc
     3061 tttgggacgg caaggataac gagggacata ctgtttctac cggaacttat attattcgga
     3121 tgaatgcgcc caattatcaa gcatcacata aaatctcctt gattaaataa actatattct
     3181 ccctgatcta acaggtgtgt atctctggta tatgcacctg ttattttttt gctcgttttt
     3241 gctattctgc attt
//
LOCUS       DAOOZV010000105         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_24224_length_3177_cov_49.962844, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000105 DAOOZV010000000
VERSION     DAOOZV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24224_length_3177_cov_49.962844"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1185)
                     /locus_tag="PLW08_07555"
     CDS             complement(133..1185)
                     /locus_tag="PLW08_07555"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HPL52486.1"
                     /translation="MNKFSAEKTQKISEVVIKILYRRFEEFPSDLSRNRNAPFHKAFL
                     QAFYDKMDRYQINNYELIGLSSWLHGLNTTLGQVFFEKVAHILSGGEKREFTSARSGA
                     LNITVKQQQAIMDIMTDLSNRNKKPDLERELQAIPKAGKNDLIETQGFSADVFWIDQN
                     GIEAIELKTVKPNSGEMKSEKQKILIGRTALQNKYPDKDVHFHIGFPFDPTVDEDIES
                     PCSYNKPRFLNSIICGNDFFDPKEVLLASELWDYLSGQKGTMKFLINIINTIATPEFM
                     DDYNIVQSFNGNYNSNIMSILEKWNLFSEMEIAQYSELIKENINNNRNLARVFNQRII
                     NPDYNRNRYNELSKLF"
     gene            complement(1190..2773)
                     /locus_tag="PLW08_07560"
     CDS             complement(1190..2773)
                     /locus_tag="PLW08_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPL52487.1"
                     /translation="MPEKEYLSIKQASKWATQKLGKKVTSSNIAYLVQYGRIKKYGEN
                     GQCYVLKEELAEYYHNISHNKKEEWTQQLGEDLNWVLAFDKYTEAETTKHVHRLHPYK
                     GKFIPQLVEYFLDTHTDKFKQEVFFHPGDIVLDPFVGSGTTLVQANELGINAIGIDVS
                     AFNVLISSTKIMGYDLQSLSFELNKLTNGLKNYLMDSKVFEFEQELLEELSKFNKKFF
                     PVPEFKYKVAHNLIDEKSYTEEKEKEFLPIYYSLIDKYAIKLQQDKSETFLDKWFSQQ
                     IREELEFMAKELRKIDNVNNRKLASIILSRTMRSCRATTHSDLATLIEPVTATYYCKK
                     HGKICKPQFSILKWWETYSKDTLKRVMQFAALRTNSTQVCVTGDSRTIDIYDKVKQVN
                     PDFAEKIKVNGINGIFTSPPYVGLINYHEQHAYAYDLFNFERNDELEIGPLFKGQGYE
                     ARKSYSESIVQVLLNCKKFLAANYNVFLVANDKYNLYPQIAEIAGMRIVNQYKRPVLN
                     RTEKDKTAYSEVIFHLREK"
BASE COUNT          986 a          580 c          458 g         1153 t
ORIGIN      
        1 gttaaaacac aggaacgaac atttacccgt aaattgttaa aaatgtgagt ggtgagatag
       61 tgctatctcg ctatttttct cttatgatac agccctaaat atattcgttt ctaaccctat
      121 atttatgtta cattaaaaca gcttgcttaa ttcattatag cgattcctat tataatcggg
      181 attgataatg cgttgattga aaacacgagc taagtttcgg ttattattta tgttttcttt
      241 tatcaactcg ctatattgcg ctatttccat ttcgctgaat agattccatt tttccaagat
      301 gctcattatg ttagagttat aattaccatt aaaactttgg actatattgt aatcatccat
      361 aaattccggc gtggcaattg tattaattat gtttatcagg aatttcattg tgcctttctg
      421 tccagaaaga tagtcccaaa gctcactggc aagcaaaact tctttgggat caaagaaatc
      481 attcccacaa ataatactat tcaggaatcg gggtttatta taactgcaag gtgattcaat
      541 atcttcatct actgttggat caaaaggaaa accaatatga aaatggacat ctttatctgg
      601 atacttattt tgcaatgcag tccttccgat tagtattttc tgtttctcac ttttcatctc
      661 acctgaattt ggtttaactg ttttcaattc tattgcctca ataccatttt ggtcaatcca
      721 aaaaacatct gcactaaatc cctgggtttc tattaagtcg ttttttcctg ccttaggaat
      781 agcttgcagt tctctttcta aatcgggttt cttatttcta ttggataaat ccgtcattat
      841 gtccataata gcttgttgct gttttaccgt aatatttaga gcccctgagc gagccgaggt
      901 aaattctctt ttttcgcctc cactaaggat atgagctact ttttcaaaaa aaacctgacc
      961 caaagttgta tttaaaccat gtaaccagct actcaatcca atgagttcat aattatttat
     1021 ctgataccga tccattttat cataaaatgc ctgcaaaaag gccttgtgaa aaggagcatt
     1081 acggttacga gagagatcag aaggaaattc ctcaaacctt ctatagagga ttttgattac
     1141 cacttccgat atcttttgag ttttttcggc agagaactta ttcattcaat tacttctccc
     1201 gtaaatggaa tataacttcc gaataagcag ttttatcttt ttcggttctg tttaaaacag
     1261 gtcttttata ttgattaaca attctcatcc cggcaatttc agctatctga ggatataaat
     1321 tatatttatc gtttgccact aaaaatacat tatagtttgc tgctaaaaat ttcttacaat
     1381 taagtaaaac ctgaacaatg ctttcgctgt aactttttct tgcttcatat ccttgccctt
     1441 taaataaagg accaatttcc agttcatcat tacgctcaaa gttaaagaga tcataagcat
     1501 aagcatgttg ttcgtgataa ttgattaatc caacataggg gggagaagta aaaattccgt
     1561 ttattccatt cactttaatt ttttctgcaa aatcgggatt tacttgtttt actttgtcat
     1621 agatatcaat agttctgcta tcaccggtta cacatacttg agtgctattt gttcttaagg
     1681 cagcaaattg cattactctt tttaatgtat ctttagaata agtttcccac cattttaaaa
     1741 ttgaaaactg tggcttgcat atttttccat gttttttgca ataataagtt gcagttacag
     1801 gttcaattaa agtagccaga tctgaatgag tagtagctct acaactcctc atagttcggc
     1861 taagaataat actggcaagt ttacggttat taacattatc aatttttctt aattcctttg
     1921 ccataaattc cagctcttct ctaatttgtt gagagaacca tttatccaaa aaggtttcag
     1981 atttatcctg ctgtaatttg attgcatatt tatcaattaa actgtagtaa attggcaaaa
     2041 actctttttc tttctcctca gtataacttt tctcatctat taagttatgt gccactttgt
     2101 atttaaactc cggaactgga aagaacttct tgttaaattt gcttagttcc tcaagcaatt
     2161 cctgttcaaa ttcaaatacc ttggaatcca tcaagtaatt ttttaggcca ttggtcagtt
     2221 tatttagttc aaaactcaaa ctctgaagat catatcccat tattttagta ctggatatta
     2281 aaacattgaa agcagaaaca tcaataccta tagcattaat acctaattca ttagcttgca
     2341 ctaaagttgt tccgctacca acaaatgggt caagaacaat gtctccagga tgaaaaaata
     2401 cctcctgctt aaatttatct gtgtgagtat ctaaaaaata ctcaactaac tgtggaataa
     2461 atttaccctt ataaggatgt aatctgtgca catgtttagt tgtttctgct tctgtatatt
     2521 tatcaaaagc cagaacccaa tttaaatctt caccaagttg ctgagtccat tcttcttttt
     2581 tattatggct aatgttatgg taatattctg ccaattcttc ttttaacaca tagcattgtc
     2641 cattctcacc atattttttg attcttccat attgaactaa atacgcaata ttggaagagg
     2701 taaccttttt ccctaatttt tgggttgccc atttactggc ttgttttata cttaaatatt
     2761 ccttttcggg catagacata tttgatctcc attctcatat atgcaaacta aaatttaaca
     2821 atttatagaa acgataagta tacatttagc gacctttcat atatgtcaag accaaaattc
     2881 tttatccagt ttctaattat tattatcaaa gctaaaaaaa tggaacgagg ataactggat
     2941 cgactggatc aataggaata gtttttttgt actgtttata agaacaagga taataaacga
     3001 agaacttcaa aataagaact cgccataaca tactaattta gataatgttc taatcgctaa
     3061 aacttcgctt ctaaaattaa tctgtgaaat tcgtgagcgg caacttttta tctatgcgag
     3121 gcaaacaaaa acgccactac attttagcag tggcgttaac agattagaaa ctatcta
//
LOCUS       DAOOZV010000106         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_29210_length_2736_cov_55.816486, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000106 DAOOZV010000000
VERSION     DAOOZV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_2736_cov_55.816486"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1397
                     /locus_tag="PLW08_07565"
     CDS             126..1397
                     /locus_tag="PLW08_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPL52488.1"
                     /translation="MKKRLFPVLLCCVLIFSACSQNTRLSKKTQKNLNKGNYEEAIQD
                     IIKTLKKNPNNATAQDLLVQSWQSYRSAEQKKIERIIQSDEINKWEQVYQEYSALQKT
                     GEEIQSLPPLINPYSGYRVNIEIPDYTEQIKQSKENAAEAHYQAGIRYAKISNDRYTQ
                     KKAALEFKAALALIPNYQDADLRYEQCRKLAIKRIAVSPFTDKSNTSGKYGAVSDILT
                     DHIVSRLISAAVNNEFVEIISRSQLETVMKEQQLSASGLVNDASSVRLGQILGANEIL
                     AGSILQISVSPERTVSVQSEDETEVVLRTEEYTDDEGNTQEREIKGKVYFRYRKFTKT
                     ASVNISTSYSILDVETGKILLQETVEVKNPWSDTWARKISGDDRALSSSTKKLLEKPE
                     PFPPSVNEMVLETLKNTGNEIVNKVSGYLLQ"
     gene            1442..2731
                     /locus_tag="PLW08_07570"
     CDS             1442..2731
                     /locus_tag="PLW08_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPL52489.1"
                     /translation="MAKRQPEWIVERPYNNEYYIAIVKVPRKAPNYVELARNNAILEI
                     STQISVQIDSDIALKETEENGIPSSEIISRIRSSSKNILKDLQLVGTYETKNDYWAYY
                     RLSKSEYSAWRKAQCNQAMAQALNLLADFDSSTSNIASGIASLLHGLELIVDYTDMDL
                     TTLYKGNEINLYNELFYRLNRLPENLSVKFATNEVDLVAKQRERKTVNIAVSYKTVNN
                     EYPCSNFPVCFNFNSGKGEIIPQAITDENGKAELIINRITSFSNPQFIEAKPNKDFWL
                     VGRENTVVKTMFNNLQFMPVILKLNVRRPKAYLEYSFDNTPGTDFRNILVKKLQDLDL
                     EVTENQNTSDYTFKVDIYNRSTNYLPLLNQYSATADAYIELLQTGNGKSIYNTNITGI
                     KSTASLPEIARKMSELNAVNEICDKVMFMLVEQYIMF"
BASE COUNT          932 a          553 c          508 g          743 t
ORIGIN      
        1 atccagtaaa ttcagttatc cagttatctg tgttctatca ttctcagtca aaatcagctt
       61 gacataaacc cttgttaatc cataaatgtt ttcaataaag ttattcatcc ctatcaggag
      121 ttttaatgaa aaaacggtta tttccggttc ttctttgttg cgtgctgatt ttcagtgcct
      181 gctctcaaaa tactcgtctt tccaaaaaga cccaaaagaa cttaaacaaa ggtaattacg
      241 aagaagcaat tcaggatatc ataaaaaccc ttaagaaaaa tccgaataat gcaacagctc
      301 aagacctttt ggtgcaatcc tggcaaagct accgttcagc tgaacagaaa aagatagaaa
      361 gaatcatcca aagcgatgaa ataaataaat gggaacaggt gtatcaggaa tattccgctc
      421 tgcaaaaaac cggagaagaa attcaatccc tacctccttt gataaatcct tattcaggtt
      481 atagagtaaa catagaaatt cccgattaca cggaacaaat aaaacaaagc aaagaaaatg
      541 ctgctgaagc gcattatcag gcaggaattc gttatgccaa aatcagtaat gataggtata
      601 cacaaaagaa agcagctttg gaatttaagg cagcgcttgc tttgattccc aattatcaag
      661 atgccgattt aagatatgaa caatgccgca aactggcaat taaaagaatt gccgtttctc
      721 cctttactga taagagcaat acttccggaa aatacggtgc cgttagtgat attttaaccg
      781 accatattgt ctcccgcctt atttcagctg cagtaaataa tgaattcgta gaaattatat
      841 cccgttccca attggaaacg gtgatgaaag aacaacagct ttccgcttca ggtttggtga
      901 atgatgccag ttccgttcgt ttaggtcaaa ttttgggtgc taatgaaatc ctcgctggca
      961 gcattttaca gattagcgtt tccccggaac gcactgttag tgttcaaagt gaagatgaaa
     1021 cggaagttgt tcttcgtact gaagaatata ccgatgacga aggaaacact caggaacgcg
     1081 aaattaaagg aaaggtctat ttccgttacc gcaagtttac caaaacagca agtgttaata
     1141 tttccacctc ctacagtatt ctggatgtgg aaacgggaaa aatactactg caagagacag
     1201 tggaagttaa aaatccctgg agcgatactt gggcaagaaa aatttctgga gatgaccgtg
     1261 ccttaagttc cagcaccaaa aaattgctgg aaaagccgga accctttcct ccttcggtaa
     1321 acgaaatggt gctggaaacg ctgaaaaata caggaaacga gatagttaat aaagttagcg
     1381 gatatttatt acagtgaagc gttttttata cctcctgctc ttaatcttta ccgcatgcag
     1441 tatggcaaaa cggcaaccgg aatggattgt agaaagaccc tataataatg aatactatat
     1501 cgctattgta aaagttcccc gtaaagcacc taattatgta gaacttgccc gaaataacgc
     1561 tattttggaa atatccactc aaataagcgt gcagattgat tccgatattg ccttaaaaga
     1621 aacggaagaa aatggcatcc cctcctctga aataatcagc agaatacgca gttccagtaa
     1681 aaatatactc aaagacctgc aactggttgg cacttatgaa accaaaaatg actattgggc
     1741 ttactatcga ctttccaaaa gcgaatattc tgcatggcga aaagctcaat gtaatcaggc
     1801 aatggctcag gcattgaatt tacttgccga ttttgattcc tccacctcta atatcgcttc
     1861 cggcatagct tctcttttgc atggactgga actgattgtg gattatactg atatggattt
     1921 gacaactctt tacaaaggca atgagattaa cctctataac gaactattct atcgtctgaa
     1981 ccgtttaccg gaaaacctta gcgttaaatt tgccaccaac gaggtagacc ttgtagccaa
     2041 gcaaagagaa cgcaaaacgg ttaatattgc cgtcagttac aaaactgtaa ataatgaata
     2101 tccctgctct aactttcctg tatgcttcaa tttcaacagt ggcaaaggag aaatcattcc
     2161 ccaagccata acagatgaaa acggtaaagc ggaattaatt atcaatcgca taacctcttt
     2221 cagcaatccc caattcattg aagccaaacc gaataaggat ttttggctgg taggaaggga
     2281 aaataccgta gttaaaacaa tgtttaataa tctgcaattt atgcctgtca tcctgaaatt
     2341 aaatgtccgc cgtcccaaag cttaccttga atacagtttt gataacactc cgggaactga
     2401 cttccgcaat attttagtaa agaaactgca ggatttggat ttggaagtta ccgaaaacca
     2461 aaacaccagc gattatactt tcaaagttga tatctataac cgtagcacta attatctccc
     2521 acttctcaat caatattctg ccacagctga tgcttacatt gaactcctgc aaaccggcaa
     2581 tggcaaaagt atttataaca ccaacataac cggaattaaa agcactgctt ccttacccga
     2641 aatagccagg aaaatgagtg aactgaatgc ggtgaacgag atttgtgata aagtgatgtt
     2701 tatgctggtg gaacaatata ttatgtttta gagtgc
//
LOCUS       DAOOZV010000107         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_31948_length_2553_cov_62.010008, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000107 DAOOZV010000000
VERSION     DAOOZV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31948_length_2553_cov_62.010008"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..2432
                     /locus_tag="PLW08_07575"
     CDS             423..2432
                     /locus_tag="PLW08_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52490.1"
                     /translation="MNKIAKIIVLIACIAIIWTGLTADEVIIGSGTQTARIPLDFFYH
                     NSLFECLYYQNELGFAQGTISRIAFYNNFVTLLNNKPTKIWLGTTNLTTLNAGWIPST
                     ELTLVFDGQVNYPSGIHIIDIELDTLFHYTGGTLVLMVQRPMDSGYFSSSDKFYCQTD
                     TMLRARNAYHDTQLLDPANPPASGFVLNGQYPKIRITYTAQSIASDLACSQVISDKVV
                     TLGATEQVQVKINNYGYEDQDNYIVNLKKGDGELLATAFGNPVSAGQTLIQTLMWVPT
                     TPGTYDIFAEVVLNGDQDLTNNQSPHINVTAFEAGFFSLTIGIGNLSGNLPLDFSYHT
                     SLFETLIYAPEIDTTGIITHLIFYNNFIENINDKPVIIWLGETDLVNLPLTWIPSSNL
                     AMVYYGTMSFPAGQNEIIFPLYPTYNYQGQNLVLMVQRPFDYNVYNSNNVFRMQSSAM
                     QRTKYIYSDQQEHDPGAPPLYAFDSYQFPQTGFIMRPPNTGFLRGTVRNGTDLVIGAT
                     VTADGSQTTYTSSAGIYSLLLSSGLHNITASYPGLNTVTRQVNITAGIVTTEDFNLEG
                     NAVEDPQTPHISTSLKGIYPNPFNPETTISFSIYRDNTPVVIELYDLKGRKVKTLINS
                     VYNRGEQQFHFFAKDEKNQDLASGVYFIRLAAPDYSKTVKALLLK"
BASE COUNT          761 a          478 c          472 g          842 t
ORIGIN      
        1 ttttcaccgg gttgaaaccc gttgttaata tctgtcgttc ctacggaact tttttcccca
       61 ataaatatag ttttgtaggt tcttttctta tcaccgggtt gaaacccgtt gttagtttct
      121 gtcgtttcta cggaactttt taaagcacaa tatattgtat ttttgctctc cgggttgaaa
      181 tctatattta taaaatatcg gttgggaaac gcatagatat tatttatctt gtgtggattg
      241 ctgctggagt aggactaaca aaggtttctg ctttcggagg taggacaaat tgcttattat
      301 tcaaaaaaat gatattactt tgtcatttct gcttcttcaa ttttataaca aaaaacggtt
      361 caaaagttgc ttcatttact ttataattac attacttgca gttaagaaac agggagttta
      421 tgatgaacaa gatagccaaa ataatcgtgc taatagcgtg tatcgccatt atctggacag
      481 gtttaaccgc cgatgaagta attatcggtt cgggaactca aacagcaaga attcctttgg
      541 atttcttcta tcataactct ttatttgagt gcttatatta tcaaaatgag ttgggttttg
      601 cacaaggaac tatttcccgc attgcttttt acaataattt tgtaactttg ctaaacaaca
      661 agcccactaa aatttggttg ggaacaacga atcttaccac cttaaatgcc ggttggattc
      721 cttctacgga gttaactttg gtttttgacg ggcaggttaa ttatccttca ggtattcata
      781 taattgatat agagctggat acattgtttc attatacagg tgggactttg gttttgatgg
      841 tgcaacgtcc tatggatagt ggttattttt cttccagtga taaattttac tgtcagacgg
      901 atactatgtt acgtgcccga aatgcctatc atgatactca attattagat cctgccaatc
      961 cgcctgcaag cggttttgtg cttaatggtc aatatcctaa aataagaatt acttacacgg
     1021 ctcaaagcat tgccagcgac cttgcctgtt cgcaagttat ttctgataaa gttgttactt
     1081 taggtgctac ggagcaggtt caggtgaaga ttaataacta tggctatgaa gatcaggata
     1141 attacattgt taacctcaaa aaaggggatg gagaactttt agctactgcc ttcggaaatc
     1201 ctgtttcagc aggacaaact cttattcaaa cattaatgtg ggttcccaca actcctggca
     1261 cttatgatat ttttgccgaa gttgtgttga atggtgatca ggatcttacc aataatcaat
     1321 ctcctcacat aaatgttaca gcgtttgaag ccggattttt ctccttaacc ataggaatag
     1381 gtaatctaag cggtaatctg cctttggatt tcagttatca taccagttta tttgagaccc
     1441 taatttatgc tccagaaata gatacaacgg ggattataac gcacctgata ttttacaata
     1501 attttattga aaatattaat gataaacctg ttattatctg gctgggtgaa acagacctcg
     1561 tcaatttacc tttaacctgg ataccatctt ctaatctggc aatggtttat tatggaacaa
     1621 tgtccttccc agcgggacag aatgaaataa ttttcccgct ttatccaact tacaattatc
     1681 aaggtcaaaa tcttgtttta atggttcagc gtccttttga ttataatgtt tataattcca
     1741 acaatgtatt ccggatgcaa tcaagcgcaa tgcaacgcac aaaatatatt tacagtgacc
     1801 agcaggaaca cgatccggga gcacctccac tttatgcttt tgattcttat cagtttcctc
     1861 aaacgggatt tattatgcga cctcccaata caggtttttt aaggggaacc gtgagaaatg
     1921 gaactgattt agtaattgga gcaactgtta cggctgacgg cagccaaact acttacacct
     1981 cttccgcagg aatttattcc ttattattga gcagtggttt gcataatatc acagcttcct
     2041 atcccggatt gaatacagtt accagacaag tgaacattac ggcgggaatt gttactactg
     2101 aagattttaa tttggaaggc aacgcggttg aagatcctca aacacctcat atttctactt
     2161 ccctgaaagg aatttatccc aatcctttca atccagaaac aactatttct ttcagcattt
     2221 atagggataa caccccggtt gtaatagaat tatatgacct caaaggacgt aaagtgaaaa
     2281 cccttataaa ttccgtctat aacaggggag aacagcaatt tcatttcttt gcaaaggacg
     2341 aaaagaacca agaccttgcc tcgggagttt attttatccg gcttgccgca cccgattata
     2401 gtaaaaccgt taaggcgctt ttgctgaaat aggcattttg tgtaaacaag atattttcac
     2461 agtatctaat atcggtttca agtagaaaag ttatgtctta aaaattctgt aagacaaagt
     2521 agttttatcc caaacaaggc agttgaggac aaa
//
LOCUS       DAOOZV010000108         2447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_33803_length_2447_cov_65.374582, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000108 DAOOZV010000000
VERSION     DAOOZV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33803_length_2447_cov_65.374582"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="PLW08_07580"
     CDS             complement(<1..2095)
                     /locus_tag="PLW08_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPL52491.1"
                     /translation="MNAYYRQPAIYKDQVVFIAEDDLWTFNLKDKVARRLTANLGMIR
                     YPQFSPDGKQIAFTSTEEGTSEVYIIPSEGGEVTRLTYLGAYLNTLGWKGNKIIFASS
                     HTSALPGYYKLFEIDSEGGSPVELPYGRANDISFGAKGVVLGRNTGNPARWKRYRGGT
                     AGYLLVDKNGDGKFKKLIDLKGNFAHPMWIGNRIYFICDHEGIGNIYSCTPEGKDLQK
                     HSNHKDFYARGAYSDGKSIIWHAGADLWLMDIKENKPRKLSFAYSSPMVQRQRKFVSP
                     PKNLEDIYLSPDGGSISFALRGKALVSGNWEGSVQQYGFKQGVRYRLPQILPDGKHLL
                     LLSDEPGYERFEIHPLRKDFEKITEEVVIIDNPDLGRVTNYVPAPKGSLVAIENHRCE
                     LIVLDLESKEFKNIDRNGFGLIWTFNWSPDGRWLTYSINRDRNTRHIIIWDSKENQSH
                     IVSKPVLNDTVPVFDPEGKYLYFASVRNLNPLRDSIQFDFIYPNSYKPYLIPLRKDLR
                     SPFIPEPKPFEIKPETPKKEEKIEEKEQKEEVKPVEIDFDGICDRLIEFPVQPGIIGG
                     LGAIKDRLFYVNMLLESSMDEESKFDLMCYDLNKLEGWTYAKDITDYDLSQDGSAILI
                     RQKDKVRVVSAKLEPKAELPADATPGKKSGYIDLNRFSVEIVPIKEWQQMFKEAWRLQ
                     TQQYWVEDMAGIDWQK"
BASE COUNT          721 a          525 c          436 g          765 t
ORIGIN      
        1 ccttttgcca gtcaattcct gccatatctt ccacccaata ttgttgtgtc tgtaaccgcc
       61 aggcctcttt gaacatctgt tgccattcct tgataggtac aatttccaca gagaatctgt
      121 tcaaatctat atagccactc tttttgccgg gtgttgcatc agcaggcaat tccgctttgg
      181 gttcaagctt ggcagaaaca acgcggactt tatctttttg cctaattaaa atagcagagc
      241 catcctggct aagatcataa tcggtaatat ctttagcata agtccatccc tccagtttgt
      301 tcaaatcgta gcacatcaaa tcaaacttgg attcttcgtc catactgctt tccagaagca
      361 tatttacata gaacaagcgg tctttgattg cacccaaacc tccgataatt cctggctgaa
      421 caggaaattc tattaagcgg tcgcagatac cgtcaaaatc aatttctacg ggtttaactt
      481 cttctttttg ttctttctct tctatctttt cctctttctt aggggtttcc ggttttattt
      541 caaagggctt gggttctgga ataaaaggag aacgcaggtc ttttcttagt ggaatcaaat
      601 agggcttata ggaattggga taaatgaaat caaactgaat actatcccgt aaagggttta
      661 aattgcgtac cgaggcaaaa tagagatact tgccttccgg atcaaaaaca gggacagtat
      721 catttaacac gggtttactt actatatgcg attgattttc tttgctgtcc caaataataa
      781 tatgccgggt gtttctatca cgatttatgc tatatgtaag ccagcgtcca tccggtgacc
      841 agttaaaagt ccaaattaaa ccaaacccgt tacggtctat atttttaaat tctttgcttt
      901 ccaaatccag aactatcagc tcacaacgat gattttcaat agcaaccagc gagcccttcg
      961 gagccggaac ataatttgta actctgccta aatcgggatt atcaataatt actacttctt
     1021 cggttatctt ttcaaagtcc ttacgcaaag ggtgaatttc aaaacgctca taaccgggct
     1081 catcggaaag caaaagtaga tgtttaccat cgggcaaaat ttgaggcaga cgataacgca
     1141 caccttgttt aaaaccatat tgttgcacac tgccttccca atttccactc actaatgctt
     1201 ttccgcgcaa ggcaaaggaa attgaacccc catcgggaga aagatatata tcctccaaat
     1261 ttttaggggg gctgacaaat ttgcgttgcc tctgcaccat cggactgcta taagcaaaag
     1321 acaatttccg gggtttgttt tctttaatgt ccatcagcca aaggtctgct cccgcgtgcc
     1381 agataatact tttgccatcg gaataggcac cgcgggcata aaaatcctta tgattgctgt
     1441 gtttttgcag gtctttcccc tccggagtgc aggaataaat attgccaatg ccttcgtgat
     1501 cgcaaataaa gtaaatccgg ttgccaatcc acattggatg agcaaaattt cctttcaggt
     1561 caatcaattt cttgaatttg ccgtcaccat ttttatccac taataaataa ccggctgttc
     1621 caccccgata tcttttccac cttgccgggt ttcctgtatt tctacctaaa acaacacctt
     1681 ttgcaccaaa agaaatatcg tttgctcttc cgtaaggtaa ttcaaccgga gaaccacctt
     1741 cactgtcaat ttcaaaaagt ttgtaataac cgggcaaagc agatgtatgc gaagaggcaa
     1801 agataatttt attgcctttc cagccaagag tgttaagata tgcgcccaaa taagttaagc
     1861 gcgttacttc tccaccttct gaaggaatta tataaacttc ggaagttcct tcttctgtgc
     1921 tggtaaaagc aatttgctta ccatcaggag aaaattgagg atagcgaatc atacccaaat
     1981 tggcagttag acgcctggca actttatctt ttaagttaaa agtccagagg tcatcttcag
     2041 caataaagac cacttgatct ttatatattg cgggttgacg ataatacgca ttcataggga
     2101 tgctcctttt tctgataaaa ttttgactgt ttagttaacg atttgaaaaa ggatattgca
     2161 gtcaagaagt ttattagaag cttaaaagta tgattgcccg ataaggtaca caaaaaatat
     2221 aatattcttt tgcttgaatt aacttcttgc atataaaata agaccgttga ataattggca
     2281 tattaaagca atgttaatat aattcaaagc ttctcccctt tgtagtttgc taaagcgtat
     2341 ttagttagat ataaaaatta tactttatcc cataataact attggaccaa gctgaaaaag
     2401 ttccaacgga acctttttta ctctggtttc ttttcaccgg gttaaaa
//
LOCUS       DAOOZV010000109         2209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_38805_length_2209_cov_79.570102, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000109 DAOOZV010000000
VERSION     DAOOZV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38805_length_2209_cov_79.570102"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            668..1675
                     /locus_tag="PLW08_07585"
     CDS             668..1675
                     /locus_tag="PLW08_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPL52492.1"
                     /translation="MKRIVLALILFIATISAWSNPIVPNLISRFWFEQENVLEIELSA
                     FVGTFQTAEIIYLDAEDSLTIQVNPNAVTVQQINVPQSGNTPASGFFTIRIPQLYQET
                     VHWGTGLENDLSPLNGTQCAVQILYSNLIGDNVYGWAKDVSPAVCGPNYCSAKFTYRL
                     ECTDLSGLPVAGVSVYNSQSAGGIFINPAITNSAGIATGELYPMKTQIWVNNPITNEI
                     AFNTQFFAEPGALIPFSVQLPVSVEENIVSIPAGKLHLYPSVLNPHWNESIRISYDTK
                     LTGKAELVLYDLKGRHLAEKVYDQNEIEWQLPKLSSGIYFVRLTNQGKALGTAKLIIL
                     K"
     gene            1672..>2209
                     /locus_tag="PLW08_07590"
     CDS             1672..>2209
                     /locus_tag="PLW08_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52493.1"
                     /translation="MKYAFLLLLLAEIMMLSALTFEAEELNFYLENNLWEMDGLFHFA
                     NYDSLSAKELIFFPVPSDSFCLPPKIKALSVQDDKEGAVTLLNQDKNGFTFLLSLPAL
                     SFCSLHLDYTQTLKSNYAKYIITTTNSWGRPIPYAKYTLHPGISVQISSLPFPLQKQE
                     GRNYIWEFFDFAPEKEFEV"
BASE COUNT          647 a          407 c          399 g          756 t
ORIGIN      
        1 gtgaaaagcg aaaagttgga aagatggaaa ggtgaaacat acattatatc tattctataa
       61 aagcaatcat tactcataat tttcccttaa tatgattttc cctttgaaaa gctatatatt
      121 aaaaattccg ttagagttag caggaattat ttcttaagtt tagaatttat gaataggaat
      181 atagtattat ccatagttga cgccgacagc aactatccta taaacaaatt gccgtaattc
      241 ctcagtgcct aaagattttg cgggtattat agaagttttt tcaacagtga cgactcccga
      301 tattttttct ttgatcaggt atagataaaa ggaactatct tgtaactaac ttttagttac
      361 ggaatatccc ctttttaacg ctcatcattt tcatttttgc accaggttca aaacctatcc
      421 ttattttctt gccttaccta cagaattttt tccctattat ttacggtctc tgaatataaa
      481 aattatataa acactatcaa attataatta tttccggtgc tctcagtgtt ttcagtggct
      541 tctggataaa taaaatcctg gcgttctatg caagctaacc cctacttatt ttgttaatta
      601 gggaataaaa attgctttat ttatatttgt gctaaatttt tagtgtttgc tcaaggaggt
      661 aagaaaaatg aaacgcatcg tcttggcatt aatattattt attgccacta tttccgcttg
      721 gtcaaatccc atcgtgccaa atttgatcag cagattttgg tttgagcagg aaaatgtatt
      781 ggagattgag ctttctgcat ttgtgggtac atttcagaca gcagaaataa tttatctgga
      841 tgccgaggac tcacttacca ttcaggttaa tcccaatgcc gtaactgttc agcaaattaa
      901 tgtgccacag agtggtaata cacctgcaag tggttttttt actatccgaa ttcctcagct
      961 atatcaagag acagttcatt ggggaacggg tttggagaac gatttaagtc cgctaaatgg
     1021 aactcaatgt gcggtacaaa tactttattc caatcttatt ggtgacaatg tttatggttg
     1081 ggcaaaagat gtttcgccgg ctgtttgcgg accgaattat tgttctgcca agttcactta
     1141 caggcttgaa tgcaccgatt taagcggttt acctgttgcc ggagtttcag tttataatag
     1201 tcaatccgcc ggaggtattt ttataaatcc agcgattact aatagtgcag ggattgctac
     1261 cggggagctt tatccaatga aaacgcagat ttgggtaaat aatcctatta ccaatgaaat
     1321 agcttttaat actcagtttt ttgctgaacc gggagctctt attccctttt ctgtgcaatt
     1381 gcctgttagt gtggaagaaa atatcgtttc tattcctgca ggaaaactcc atctatatcc
     1441 ttctgtatta aatcctcatt ggaacgaaag tatccgaatt tcttatgata ccaagcttac
     1501 aggcaaggca gaacttgttt tatacgattt gaaaggacgc catcttgcgg aaaaagttta
     1561 tgaccaaaat gaaattgaat ggcagctgcc aaaactttcc agcggcattt attttgtgcg
     1621 tcttacaaat caaggtaagg cattggggac agctaaactt attatactta agtgaaatac
     1681 gcttttctcc tgctgttatt agctgaaatt atgatgcttt ccgctctcac ttttgaagcg
     1741 gaagagctga atttctatct ggaaaataat ttatgggaaa tggatggtct atttcatttt
     1801 gccaattacg attcgctttc cgctaaggaa ttgatctttt ttcccgttcc cagtgatagt
     1861 ttttgcctgc ctcctaaaat taaagcatta tctgttcaag atgacaagga aggagcagta
     1921 actctgctaa atcaagataa gaacggtttt acttttctcc tttctctgcc tgctctttct
     1981 ttttgttctt tgcaccttga ttatactcaa acccttaaaa gcaattatgc caaatatatt
     2041 atcaccacta caaattcctg gggcagaccc ataccttacg ctaaatatac tttgcatcct
     2101 ggcataagtg tgcaaataag ttctcttcct ttccctctgc agaaacaaga gggaaggaat
     2161 tatatctggg aattttttga ttttgctccc gaaaaggaat ttgaggtta
//
LOCUS       DAOOZV010000110         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_45587_length_1962_cov_66.218144, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000110 DAOOZV010000000
VERSION     DAOOZV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45587_length_1962_cov_66.218144"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1019)
                     /locus_tag="PLW08_07595"
     CDS             complement(69..1019)
                     /locus_tag="PLW08_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPL52494.1"
                     /translation="MKLWITYLKAVLAMLFWSATFVWFKIAYKTYLPYEVTFLRLLLA
                     TILLFPFVWLTGKREKVPKKDLLHLMLVSFCEPFMYFIGEANGMRIVSSTLGSLIIST
                     IPIFSAVGAWLILREKLPLLVVLGLILSTSGVAVMSLGSDNFSATFKGILLLLIAVFS
                     GVFYGITVRNLTLRYNPFTIVAWQNFFGMLYFLPLFLVVDGKHFFSVQHSLEGLATIA
                     AMSIFASVIAFILYTVVIRDLGVAKSNIFTNLIPVFTVALAYIILGDKLGLPGWIGLI
                     LAIIGLFLSQYPDWQKYRKQEKTELLSVVENAETNISDEL"
     gene            complement(1019..1618)
                     /locus_tag="PLW08_07600"
     CDS             complement(1019..1618)
                     /locus_tag="PLW08_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPL52495.1"
                     /translation="MHKLTKLNTPIFWAEGHPGLLKRENWQIEVSGSCEKPGIFTWES
                     LLALPQKEVKGRLTSVTRWSVEGFWQGIPLSEILQAVAVKENCRYIRFWSINKIYDTS
                     IPIEIANKEKSLLAYSFDGELLTEDYGGPIRAFIPYLWGYKSAKSVVAIELMDYYVPG
                     FWEKRGYTDSGEIEAGPCRDLNDGGKIKYIPDGEVLKFN"
BASE COUNT          663 a          386 c          343 g          570 t
ORIGIN      
        1 atttttattt atggcttttt aaaggggaaa aagtaatata ccgcataatg gttcttaact
       61 gcaaagctct aaagttcgtc ggaaatattg gtctctgcgt tttcaacaac ggatagcaat
      121 tccgtttttt cctgtttccg gtatttttgc caatcgggat attgtgataa aaagagaccg
      181 ataatggcta aaataagtcc tatccatcca ggcagaccca atttatcacc taaaataata
      241 taagccagag caactgtaaa aacgggaata agattggtaa aaatattgga ttttgccact
      301 cccaaatcgc gaataaccac tgtataaaga ataaaagcaa ttacggaagc aaaaatagac
      361 attgcggcta tagtcgcaag tccttccaag gaatgctgaa cactgaaaaa atgctttccg
      421 tcaaccacaa gaaacaaggg caggaaatag agcataccaa aaaaattctg ccaggcaact
      481 atcgtgaaag gattgtaacg caaagttaaa tttcgcaccg ttattccata aaaaaccccg
      541 gaaaaaactg caatcagcaa taaaagaata cctttaaaag tagcggaaaa attatcactg
      601 cctaaactca taaccgctac tcccgaggtg gataaaataa gccctaaaac cactaataaa
      661 ggcaattttt ccctcaaaat cagccaggca ccgacagcag aaaaaatagg aatagtggaa
      721 attatcaaac tgcccaaagt ggaagaaaca attctcattc cgtttgcctc gccgataaaa
      781 tacataaagg gttcgcagaa agaaacaagc atcaaatgta aaaggtcttt cttgggaact
      841 ttttcccgct ttcccgtaag ccacacaaag ggaaaaagca aaatagtagc cagcaacaaa
      901 cgcagaaaag ttacctcata aggtaaatat gttttataag caattttgaa ccaaacaaag
      961 gttgcagacc agaaaagcat tgccaaaacg gcttttagat aagtaatcca gagtttcatc
     1021 agttaaattt cagcacttca ccgtcaggaa tatattttat ttttccgccg tcattaaggt
     1081 ctctacaagg tcctgcttca atttcaccac tatctgtata acctcttttt tcccagaaac
     1141 cgggaacata ataatccata agttcaatcg ctacaacgct ttttgcggat ttataacccc
     1201 acagataagg aatgaaagcc cggattggac ctccgtaatc ctcggtgagt aattctccgt
     1261 caaaactata tgccaaaagg gatttttcct tattggcgat ttctatggga atagatgtgt
     1321 cgtaaatttt gttgatactc caaaaacgaa tgtagcggca attttcttta actgcaacgg
     1381 cttgtaaaat ttccgagagg ggaattccct gccaaaatcc ttctacagac catcttgtta
     1441 cgcttgttaa gcgccctttc acttcttttt gaggcaaagc caataaactt tcccaggtaa
     1501 aaatgcctgg tttttcacag ctaccgctta cttcaatctg ccagttttct ctctttaata
     1561 agccgggatg accctcagcc caaaaaattg gtgtattcaa ttttgttagt ttatgcattt
     1621 cttttctcct atagctattt ggagtgatta aagttataat ttgcctattt ttaactttag
     1681 cgtattgtca atgtttttct ttagcacatt tcaatttggg gattagatag tggattagga
     1741 aagcttgaaa aagggattag aattacacaa tgcataaaat attaataagt tagccatata
     1801 ttttgtccac aagggcgtaa aatgcataat tgcttgttat ttagttgatt aagtaacgac
     1861 tccgtaacac ttccgtaaca ctgccgtagt tccgttacgg agttgttact tgattgttac
     1921 aatattataa gggggtcttg caagtataaa tttaatttta ta
//
LOCUS       DAOOZV010000111         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_46444_length_1937_cov_63.943677, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000111 DAOOZV010000000
VERSION     DAOOZV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46444_length_1937_cov_63.943677"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLW08_07605"
     CDS             complement(<1..788)
                     /locus_tag="PLW08_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HPL52496.1"
                     /translation="MKIYISTDMEGIPGTFNWEQEKTNRPEVLKNYQTHITDCLNAIL
                     YSGFKEFIEEITIADSHAGGDNLSYEITALDKRISLISGGPRPQYMMPALDSDYAMVF
                     LLGYHSGTGTWKGNMDHTYSNSKIFRIWINDIPMNEAIINSAYAGYKGVPVALISGDK
                     ALEEQIAVTMPWVHFVRTKEAVAKFAAKNYSSIIVKEDLVKAVQTSLAQKDIPLYTFS
                     SPIRLKIEFHSTSMTDCAALMPHTKRLDGRTIEYVDDDYGVIFEA"
     gene            complement(785..1702)
                     /locus_tag="PLW08_07610"
     CDS             complement(785..1702)
                     /locus_tag="PLW08_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPL52497.1"
                     /translation="MQLILHLSPRIKRAFDKRKPILAMESTVLTHGLPYPKNLDILKT
                     LEELCQELDVEPATIIVLDGKIHIGMTEAEKEILQCKLESGEEVGKFNLRDIPYAVAN
                     KKSGGTTVSATMLLANKAGIKVFATGGIGGVHKFWNETLDISSDLKALAEIPVIVVSA
                     GCKAILDIPATLEVLESYSVPVLGWKTDEFPAFYARTSGKKIEKIESAKDVVNIYQAM
                     QELYLHPTGILVANPVPEKDEIPFAEIEKYISAVIAEAKTKNITGKELTPFLLATLAE
                     KTKGQSVQSNLALLKNNVIIGAQIAKELQ"
     gene            complement(1758..>1937)
                     /locus_tag="PLW08_07615"
     CDS             complement(1758..>1937)
                     /locus_tag="PLW08_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPL52498.1"
                     /translation="QNSKSVIVRVNDRGPFNRGRDIDLSYAAAKEIGMLSAGVIPVQV
                     EILEQVYTDTLLAKN"
BASE COUNT          524 a          412 c          359 g          642 t
ORIGIN      
        1 gcttcaaaaa taaccccgta atcgtcatcc acatattcta tagttcttcc atcaaggcgt
       61 tttgtatgag gcattaaggc agcgcaatcg gtcatagaag tagagtggaa ctcaatcttc
      121 aggcggattg ggctggaaaa agtatagagg ggaatatctt tttgggctaa agatgtctga
      181 acggctttta ctaagtcctc tttcacgatt atagaagaat aatttttagc ggcaaatttg
      241 gctactgctt cttttgttct gacaaaatgc acccaaggca tagtaacggc aatttgttct
      301 tccaatgcct tatctccgct aattaaagca accggaacac ctttataacc agcataagcg
      361 gaatttataa ttgcctcgtt cattggtata tcatttatcc agattctaaa tattttactg
      421 ttggaatagg tgtgatccat attgcctttc caggttcctg tgccggaatg atagcccagt
      481 aaaaacacca tagcgtaatc actatctaag gcaggcatca tatattgggg tcggggtccg
      541 cctgaaataa gcgaaattct tttatccaat gcggttattt cgtagcttaa attatctcct
      601 ccggcgtgag aatcggcaat agttatttct tcaataaatt ccttgaatcc gctgtataaa
      661 attgcattca agcagtccgt tatatgtgtc tgataatttt ttaggacttc agggcggttg
      721 gttttttcct gttcccagtt aaaagttccc gggattcctt ccatatcggt agaaatgtaa
      781 attttcattg tagctcctta gcgatttgtg cgccgattat aacattgttt tttagcagag
      841 caaggttgct ttgaactgat tgcccttttg ttttttccgc caaagttgcc agcagaaaag
      901 gagttagttc cttgcctgta atattttttg tctttgcttc ggcaattacc gctgatatat
      961 atttttctat ttccgcaaaa ggaatttcat ctttttccgg caccggattt gccaccagaa
     1021 ttcctgtagg atgcagatac aattcctgca tcgcttgata gatatttaca acatctttgg
     1081 cgctttcaat tttttctatc ttttttccgc ttgttcttgc atagaaagca ggaaattcat
     1141 cggttttcca tcctaaaaca ggaacgctat aactttccag aacttccagc gtggcaggaa
     1201 tatccaaaat ggctttacac ccagcagaaa caacaatcac cggaatttct gcaagtgctt
     1261 tcaggtctga agaaatgtcc aatgtttcgt tccagaattt atgcactccg ccgattccac
     1321 ctgtagcaaa aacctttatt ccggctttat tggcaagcag cattgtggca gaaacggttg
     1381 ttccaccgct ttttttatta gctacagcat aaggaatatc ccgtaaatta aatttaccca
     1441 cctcttcccc gctttccagt ttgcattgga gaatttcttt ttctgcttct gtcattccaa
     1501 tatgaatttt cccgtccaaa acaattattg tagctggctc aacatcaagc tcctgacaga
     1561 gttcttccaa tgtttttaat atatccagat ttttaggata gggcaagcca tgagtaagca
     1621 cagttgattc cattgccaga attggttttc gcttatcaaa tgcccgtttt atacgcggag
     1681 ataaatgtaa aataagttgc atcctaagtt acctgccctt ttccctgaca tgttgaaaat
     1741 aagaattaat aatgttttta attttttgcc agcagagtgt ctgtataaac ctgctctaaa
     1801 atttccacct gaacaggaat aacccccgca gaaagcatcc ctatttcttt ggcagcagca
     1861 taagaaaggt ctatatctct gccacgattg aagggacctc tatcgtttac acgcacgata
     1921 acggatttgc tgttttg
//
LOCUS       DAOOZV010000112         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_49271_length_1859_cov_53.723392, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000112 DAOOZV010000000
VERSION     DAOOZV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49271_length_1859_cov_53.723392"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLW08_07620"
     CDS             complement(<1..299)
                     /locus_tag="PLW08_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52499.1"
                     /translation="MKKKQLTLLAILLCTILITTAQQPWRFHIAFEDGSGQRDTLWMV
                     FDTTATEGIDYQLGEGTPEYDPNAFNVFMYNMNVDSTKVKAYPYWIYPTMSTDED"
     gene            complement(497..964)
                     /locus_tag="PLW08_07625"
     CDS             complement(497..964)
                     /locus_tag="PLW08_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52500.1"
                     /translation="MNYILTFLLLVLVILYGITRMGKEINYYQLNDVFYLHKIDYFSS
                     YSISIKNNNNFDDIIKNVVAMAINKNDIIVKYVDKEETNIAVLRNDSIIYHGIGQINN
                     SELSFLSPWQIVEERTLSNKSRLFNQILIFSIIVLGFYMLMRVFNLRRLIKKP"
     gene            complement(1031..1723)
                     /locus_tag="PLW08_07630"
     CDS             complement(1031..1723)
                     /locus_tag="PLW08_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL52501.1"
                     /translation="MKTFFKLNGLTSIFILIVLIGCNKSQDQITKEDNLKSNFVNPKE
                     DVGVLHNEALKYVLENTTEIPITSETKSFVEQILNEKYQNTSLSSIPAFPDNFNDLDP
                     YVWINQFNVSEAYKVEVKKTFDLFRNANNLNEILTSIEDREIQANTIFSGSDLDLYYE
                     HLAVARHTAIFWSPEDQGGLNGIQYVAAGRFKSTKAINWWKVLGVDCVGGCMGGAVGY
                     AGASAISVIMQL"
BASE COUNT          610 a          326 c          262 g          661 t
ORIGIN      
        1 aaatcctcat cagttgacat cgtagggtaa atccagtaag gataagcctt aacttttgtt
       61 gaatctacat tcatattata cataaagaca ttgaatgcat tggggtcata ttcaggtgtc
      121 ccttccccaa gctggtaatc aattccttct gttgctgtag tatcaaacac catccaaagt
      181 gtatcgcgct ggccactgcc atcctcaaaa gcaatgtgga aacgccaggg ttgctgggct
      241 gttgtaatta aaattgtaca aagcaaaatt gctaagaggg ttaactgttt ctttttcaca
      301 attgagtttg tttaagggtt taaaattaca actaaaatta atgtattcac ttttggccaa
      361 ttttacatta cacctaaagg ttatagctat gaagcgtggc ggttttggaa tacaaatctt
      421 tcaaggtaca ctaatgtata tttgaaaacg aatatttcaa attactacca aacccgccat
      481 gctttatgag cttttgttag ggctttttta ttagtcttct aaggttaaat actctcatta
      541 gcatataaaa tcctaaaact ataatactaa atatcaatat ttgattaaat aacctggact
      601 tattagataa tgttctctcc tcaacaattt gccatggact taagaaacta agctcagagt
      661 tatttatttg acctatacca tggtaaataa tgctgtcatt ccttaaaaca gcaatatttg
      721 tttcttcttt atctacatat ttaacaataa tatcattttt gtttatcgcc atagctacta
      781 cattttttat aatatcatca aaattattat tattcttaat tgagatagaa taagaagaaa
      841 aataatctat cttgtgtaga taaaacacat catttaattg ataatagttt atttctttgc
      901 ccattctagt aattccatat aatatgacta aaacaagtag taaaaatgtt aatatgtagt
      961 tcatttttct ttttgacata attcaagaag ttaaaagggg gaactttttc aagttcccct
     1021 tgattgtaca ttagagttgc ataattacag atatggctga agccccagcg tatccaacag
     1081 cacctcccat acatccaccg acacaatcaa caccaagaac tttccaccaa ttaatagctt
     1141 tcgttgattt aaacctaccc gcggcgacat actgaatacc atttaagcca ccctgatctt
     1201 ccggagacca gaatattgct gtatgccttg caactgcaag gtgttcataa tataaatcta
     1261 agtctgatcc actaaatatt gtattagcct gaatttctct gtcttcaatt gatgttagaa
     1321 tttcatttag gttgttagca ttcctaaata aatcaaaagt tttctttact tcaaccttat
     1381 aagcttcgga aacattaaat tgattaatcc aaacatatgg atccaaatca ttaaaattat
     1441 ctggaaaagc aggaatagaa cttaatgaag tattctgata tttttcatta agaatctgct
     1501 caacaaatga ctttgtttcc gatgtgattg gtatttcggt cgtgttttcc aaaacatatt
     1561 tcaaggcttc attatggaga actccaacat cttcttttgg atttacaaaa ttacttttaa
     1621 gattgtcttc ttttgtaatc tgatcctgtg acttattaca gccaattaag acaattaaaa
     1681 taaaaattga tgttaatcca ttcagtttaa aaaaagtttt cattttgtat aaatttaaaa
     1741 taatgcctta ctttgtttcc aaggagtgtt caggtcaaat ttttctcctg ttatagacaa
     1801 aattgttagt tctctattat ttttttctcc caaacagaga acatctctca ggtatggtt
//
LOCUS       DAOOZV010000113         1825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_50649_length_1825_cov_49.031073, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000113 DAOOZV010000000
VERSION     DAOOZV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50649_length_1825_cov_49.031073"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLW08_07635"
     CDS             <1..359
                     /locus_tag="PLW08_07635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPL52502.1"
                     /translation="KSFVFDFIEPYRILVDEPVFYIFSRHKFSPDFIEPVHQGVLLST
                     AGKKFLAPLLLEHFDEIIRYRNKNRKRLDMIQTDAHAFANFLIDKRENYLETSINRKL
                     QNFLNSNFADNGEEEC"
     gene            353..643
                     /gene="cas2"
                     /locus_tag="PLW08_07640"
     CDS             353..643
                     /gene="cas2"
                     /locus_tag="PLW08_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPL52503.1"
                     /translation="MLTWVMYDIVKDKIRNKVSKCCEQAGIYRVQYSVFLGEISRTKC
                     KELSCQIEELINPEIDRVYIFPMCNEDFKSCQLLGQAFDPALITDEIKAFFL"
     gene            640..1230
                     /gene="cas4"
                     /locus_tag="PLW08_07645"
     CDS             640..1230
                     /gene="cas4"
                     /locus_tag="PLW08_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPL52504.1"
                     /translation="MNDFQFITPSEVMEYLFCPRFVYYMNILKIEQHEHRRTLVNKGR
                     DIHKLKMVQNKEYLRKKAGAIDKLTDVYLSSEKLKLVGKVDEVLFLADGSAAPLDYKY
                     AFWENKVYKTLKYQQILYALLIMENFQVPVYKAFIVYTRSQNHLEELAITPKMLDKAK
                     LILDEIFMIINLEKYPKPAKAKRKCLDCTYRNLCSV"
BASE COUNT          595 a          294 c          331 g          605 t
ORIGIN      
        1 agaagtcctt tgtctttgat tttatagagc cctatcgtat tttggtggat gagcccgttt
       61 tttacatttt ttcccggcat aaattttctc ctgatttcat tgaaccggtt catcaaggtg
      121 ttttacttag cacagcgggg aaaaaattcc tggctccttt gttattagaa cattttgatg
      181 aaattattcg gtaccgtaat aaaaaccgca aacgccttga tatgattcaa accgatgctc
      241 acgcttttgc caatttctta attgataaaa gggaaaacta tctggaaacc tcaattaata
      301 gaaaactgca aaatttcctg aacagcaatt ttgcagataa tggagaagaa gaatgttaac
      361 ttgggtgatg tatgatatcg ttaaagataa aatccggaat aaagtttcca aatgctgtga
      421 acaagccgga atatacagag ttcaatattc tgtctttttg ggggaaatta gccgcaccaa
      481 atgcaaagaa ttaagctgtc agattgagga actaatcaat cctgaaatag accgggttta
      541 tattttccca atgtgtaatg aggactttaa aagctgtcaa ttacttggtc aggcatttga
      601 tcccgctctt attacagatg aaattaaggc atttttctta tgaacgattt tcagtttatc
      661 actccttccg aagtgatgga atatctcttc tgccctcgtt tcgtttatta tatgaacatc
      721 ctgaaaattg aacagcatga acacagaaga accctcgtta ataaaggcag agatattcat
      781 aaattgaaaa tggtgcaaaa taaggaatat ctgcgtaaaa aagcaggcgc tattgataaa
      841 cttactgatg tttatctttc ctccgaaaaa ttgaaactgg tgggaaaagt tgatgaagtt
      901 cttttcctgg cagatggcag tgccgcaccc ctggattata aatatgcctt ttgggaaaat
      961 aaggtctata agaccctaaa atatcaacaa atcctctatg cgcttctgat tatggaaaac
     1021 tttcaggttc cggtgtataa agcatttatt gtgtatacca gatctcaaaa ccatctggaa
     1081 gaattggcta tcactcctaa aatgctggat aaagctaaat tgatccttga cgagattttt
     1141 atgataatta acttggaaaa atatccaaaa cctgccaaag caaaaagaaa atgcctggac
     1201 tgcacttatc ggaatttgtg ctctgtctga atatgagatt tattcacata tatgttttta
     1261 tgtatagagt gtttgctgga ataagaaaga aaattatgga aatccagctc ttgccgttag
     1321 aatgaataaa acatcattag ttaatttaat ggtgttatca ttccaccctt gatgtggaat
     1381 ccatagattt atagtgaaaa ctattaatgc taatttactt tcgtccctta tcattaataa
     1441 gatattgttt taatttggat gaaaaaatta acattatact tgctttttaa agataccgtt
     1501 tatcttaatc taagaagaat ttattgattt ttttggtacc ttttcgctct tttatagatt
     1561 tatagagtgt ttgtgcctgc ccgaaattgc cttttggctc atttgggagc tggtttttta
     1621 tcaaaactat cagtttggat tatctgaaaa accggtttaa gtttgttatt tgtgaggcac
     1681 ttatataaga agatgagttt ttactctaca gtccattaaa tcaaggattg aaacaagcat
     1741 aggaagtggg ataaaaataa gcataaggat gtttttactc tacagtccat taaatcaagg
     1801 attgaaacca atgagtaacg cacaa
//
LOCUS       DAOOZV010000114         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3528 NODE_59581_length_1636_cov_44.676787, whole genome
            shotgun sequence.
ACCESSION   DAOOZV010000114 DAOOZV010000000
VERSION     DAOOZV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865263
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 17:46:07
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,530
            CDSs (total)                 :: 1,485
            Genes (coding)               :: 1,485
            CDSs (with protein)          :: 1,485
            Genes (RNA)                  :: 45
            tRNAs                        :: 42
            ncRNAs                       :: 3
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59581_length_1636_cov_44.676787"
                     /isolate="AMR_MDS_3528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1397)
                     /gene="wecB"
                     /locus_tag="PLW08_07650"
     CDS             complement(342..1397)
                     /gene="wecB"
                     /locus_tag="PLW08_07650"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPL52505.1"
                     /translation="MKIVQIVGARPQFIKLAPLSRKVRKHHQEVIIHSGQHYDIQMNE
                     VFFRDLEIPAPDYNLNVGSGSQSTQTAGILNALEPVLIKEKPDLTIVYGDTNTTLAGA
                     LTSAKLGIKTAHIEAGLRSYNKSMPEEINRIVADHISDILLAPTETAMHNASKEGLLE
                     KTFLVGDIMVDSLSFGIKKAEKEANVLSTLQLEDENYCLLTLHRPYNVDDPDNLYHIL
                     EGLDALQQLIIFPVHPRTLNILNNMKNNRFSNIRLIEPQAYLDFLLLMKHSRMIFTDS
                     GGIQKEAYILQKPCVTLRPETEWVETVQSGWNLLLPVFSRDFPFVVNNFKPPETHPAL
                     FGENVAEKIMQILERKG"
BASE COUNT          521 a          281 c          299 g          535 t
ORIGIN      
        1 taaacacgga gaaaaagttt tttaaagtag tagctcaaag agttgtaaga taaaattatg
       61 tagccggagg atgctgttcc tccgctataa gtgaaaagtt gaaaaagtgg aaaggtgaaa
      121 caaggtttgg acatcgtaaa aaaaggatgc gaagctacaa cgagctccgg ctacacaaaa
      181 agtcaggacg atttagcaag gcaaaatggg ttggaaaaat tccgatttgc caaagaaaca
      241 gaaggtttct aaatgaaatt ggggttttaa gacaaccctt ttttctattt accgacttgt
      301 ttttctttag ggtattgttt ggagcaatgc tgagataata tttatccttt tctttccaga
      361 atttgcatta ttttttccgc tacattttca ccgaagagag caggatgagt ttctggtggc
      421 ttaaaattat ttacaacgaa aggaaaatcc cgagaaaaaa cgggtaacag aagattccat
      481 ccgctttgga ctgtttctac ccattctgtt tcaggacgaa gagtaacaca gggcttttgt
      541 aaaatatatg cttctttttg aattcctccg gaatcggtaa aaatcatccg agaatgtttc
      601 atcagaagca gaaaatccag atatgcttga ggttctatca aacggatatt actaaaacgg
      661 ttgtttttca tattattaag aatattaagc gtgcggggat gaaccggaaa aattattagt
      721 tgttgtaagg catccaatcc ttcaagaata tgatataaat tatcaggatc atctacatta
      781 taaggtctgt gcaatgtcag aagacagtag ttttcatcct ccaattgcaa agtagataag
      841 acatttgctt ctttttccgc tttttttatt ccgaaagaaa gtgaatcaac cattatatcc
      901 ccaaccagga atgttttttc cagcaaacct tctttgcttg cattgtgcat agctgtttct
      961 gtgggagcta ataaaatatc gcttatatga tcagctacaa tcctgtttat ctcttccggc
     1021 atacttttat tataacttcg taatccggct tctatatgag ctgttttaat tcccagtttg
     1081 gcagaggtta aagctcctgc aagagtagta ttggtatcgc cataaacaat tgttaggtcg
     1141 ggtttttctt ttattaaaac tggctccaaa gcatttaata tacctgcagt ttgagtgctt
     1201 tggctgccgg aaccaacatt aagattataa tccggagcag gaatttccaa atccctgaaa
     1261 aagacctcat tcatttgaat atcgtaatgc tgtccactgt gaataattac ttcctgatgg
     1321 tgttttctaa ctttccggga aagaggagcc aattttataa attgaggtct ggctcctaca
     1381 atttgcacta ttttcatata taaatcctta tctatgtata actaatatag ctttaaactt
     1441 gaatatgatg taatcaatat agattcctgt ttcctctaaa ataacaggtt atctgtatat
     1501 cttaatcttc tttgttcctt aataattcaa aggtttttac tgtcaagcag gaatccaaag
     1561 ataaaaaagc agttaaaaaa aacatatcaa aaatgatgaa gcccgtaagt tactttcggg
     1621 cttcctctaa ttatct
//
