LOCUS       DAOPAF010000001        84832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_313_length_84832_cov_43.436427, whole genome shotgun sequence.
ACCESSION   DAOPAF010000001 DAOPAF010000000
VERSION     DAOPAF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 84832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 84832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84832
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_84832_cov_43.436427"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..968)
                     /locus_tag="PLQ77_00005"
     CDS             complement(162..968)
                     /locus_tag="PLQ77_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HPL09091.1"
                     /translation="MDAQKIPGKVIIGAVIGFAAILLLLSTYFSIEAGDRGIVLRFGQ
                     VNRVVEPGARFKIPFAEEVVRMSVRVQKTTTKTEAASRDLQTVQTTMVLNYSLDPART
                     GEMYANIGLNFNERVIDPAVKESFKAAAARYTAEELISKRETLKTEVRNYLRERLHVF
                     GINIVELSVTDFEFSAEFNRAIESKQTAEQNALRAKRDLERIKVEAEQKVTSAKAEAE
                     ALRLQRQVISPELIQLRQIEAQIKAIEKWDGKLPNVTGGAVPFIQVEKPK"
     gene            complement(1033..3750)
                     /locus_tag="PLQ77_00010"
     CDS             complement(1033..3750)
                     /locus_tag="PLQ77_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPL09092.1"
                     /translation="MESIICKHWHHCPGSEVTTLLNTDPEKGLSPFEAGHRLKKYGPN
                     AVTVKKQQGPLMRFLLQFHQPLIYILMAAGAVTLFLQEWVDSSVILGVVIINAVIGFV
                     QESKAQSAIESLKKMMTTQTTVLRDGRWQEMDAVNLVIGDIVQIQSGNKVPADVRILR
                     CRDLQVDESALTGESVPVIKKEETLDPETILADRRNMAYAGTLVTYGQARAVVVATGD
                     KTETGRISELISSAADLSTPLTIKIASFSKILLIAILVLAAATFAVGMWRGESAVDMF
                     MAAVALAVGAIPEGLPAAVTITLAIGVNRMAKKRAIIRKLPAVETLGSTTVICSDKTG
                     TLTENQMTVKKIFTGGASYDVEGNGYAPEGHLSANGKELTGDLPPGLRQTLTAGLLCN
                     DSRLIQEEDAWRIEGDPTEGALITAAGKAGLTPEKTGADMPRLDEIPFESERQFMATL
                     HRSTAGGKNILYIKGAVEKILEACADAMASEGGKTAFDKENLPARVDAMASEGLRVLA
                     LACKETDKDHLEVEDCLSGLTFLGLQGMIDPPRKEAIEAVRSCRRAGIAVKMITGDHA
                     VTATEIARQLNIGSLKGKDEAAPRTLTGKELEKLTDEELIAAAEDTSVFARVAPEQKL
                     RLVEALQARSHIVAMTGDGVNDAPALKQANIGIAMGITGTDVSKEAADMVLTDDNFAS
                     ITRAVEEGRRIFDNLVKFITWTLPTNGGEGLVILAAIVTGVTLPILPVQILWINMTTA
                     VLLGLMLAFEPGESDVMNYPPRDPQSPILNATVITRILLVTAIMLAAVFGIFVWQKQQ
                     GYSLAEARTVAVNLFVMIELAYLFNCRSLTKSMFRIGFFSNPWVLAGSAAMIVLQLLY
                     TYTPAMNRVFQSEPISGRDWLMIIAISIIVYVVVGAEKWLRRGAFAKTH"
     gene            4042..5289
                     /locus_tag="PLQ77_00015"
     CDS             4042..5289
                     /locus_tag="PLQ77_00015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPL09093.1"
                     /translation="MSGKTLILGYDAVSSLGTDLEIQWRRAAAGESGIGPLTRFPYGD
                     DFPVRVAGEVEDVDVRPYPFLQPREMAHWTSPIFKYALLVVHRALAKSGIDITPDVAP
                     RVAVTFSSAVGGQDAVLKADRQMIAENKLPHPFANPNSCINMVGGKVSILTGATGPIC
                     ATITACATGVTSMIIGGMMIQQNLADVVIAGAVDFPLVEPILAGFATMNGAYRPKEGR
                     PHEAPEKVSRPFSANRRGFVVSEGAGAVILASPDFARAHGLAARIEIAGFGMTSDAGH
                     FVAPNPATVERCIAVAIADAGLTSAEVDAVNAHATSTRIGDKVEADALTQIFGAKAPP
                     TSANKSQLGHAMGASSAIETILAIEGMLRDTVLPTVNYEPDPAIHLDCVPGTARKLCQ
                     EFVLKNAFGFGGCNSCLLLRRIA"
     gene            5308..6573
                     /locus_tag="PLQ77_00020"
     CDS             5308..6573
                     /locus_tag="PLQ77_00020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPL09094.1"
                     /translation="MKPPQNRRVFIVGYGAATPLGSTFAKTWERAVQGDAGFRKVTRC
                     KVESACDIVGEIPDWSPRNLDFSDAREVYNWNAAFVLLTMAVCKEALQNAGIVMDETI
                     APRMACLIGSALNGTDAFRAAMHDLEHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPQGACASGNHAIGIGARMIRDGDCDFVLAGGADAPILPELIHGFTNMNATIKVH
                     AGDRAAADPAQASRPFSIDRRGFVLSEGAGVVVLAAEEVIRAHGLEPRAEVLGVGWTS
                     DAYHYTSPNPATIVRALRETIADAGLQPADVQYVNAHGTSTAKGDRTEIQCLREVFGG
                     LMEMIPVSSNKSQLGHTLGATAAIEAALAVEGMRRGILLPTINHLPDPEFADIDVVPN
                     TARKQTHEIVLSNAFGFGGTNCCVIFRGV"
     gene            6575..7060
                     /locus_tag="PLQ77_00025"
     CDS             6575..7060
                     /locus_tag="PLQ77_00025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPL09095.1"
                     /translation="MKPKPFLPETCKMDERFVRDRTTGLVWHRTPYRVLYADTDRSQV
                     VYHSNYLRYFEVGRTSLMRDLAYPYREIEESGYVYPIYDLGISFYQPLVYDDVMHIHT
                     RPVNLERVRLQFDYVVTRADAGRLVCTGFTRHCAANAAGRPVAVDPKTIRLWQTFPDP
                     V"
     gene            7057..7896
                     /locus_tag="PLQ77_00030"
     CDS             7057..7896
                     /locus_tag="PLQ77_00030"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HPL09096.1"
                     /translation="MKQIFSFKLPIPPYLRDHCVEGQAVFPAVESLALLAREVQNRRP
                     GALVNRLADADFPKMLALPEEADELPVRIEIEERENGIHASLQTTVSAKTASIRRALV
                     HARVTFVRKDAPPEAPMPFQASGTTGDDSIPVAADSVYRKWIPFGPSYRNLVGTLAVS
                     REGVSAEISGGSGEADDTPLGSPFAPDAAMQAACVWGQRFAGIAAFPTGFDHRIIYRP
                     VKNGETCFARVIPVNGNRGFLVFDVWIFDRREMLCESIRGLRMSDIFRGRLKTPRRIG
                     EGE"
     gene            7893..8450
                     /locus_tag="PLQ77_00035"
     CDS             7893..8450
                     /locus_tag="PLQ77_00035"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPL09097.1"
                     /translation="MINVIWICCAAYLAGSVNFAILFFKIAGRADPRLSFSGNAGTTN
                     VYRLAGMPWAVLVFALDIGRAMAVAALAAYFLDGGWLIWAGFFLVLGNSFPCFHGFRG
                     GKGVANYLGFTVLTVPVAALSAAWLWVLAYWLKRAPFIASFFMTAALSAGHVVSSSGD
                     WLAAAGAAAIFALIVFNHRKNIREY"
     gene            8561..9505
                     /locus_tag="PLQ77_00040"
     CDS             8561..9505
                     /locus_tag="PLQ77_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL09098.1"
                     /translation="MRASTKALMAKHGWRIDRAIHNYLYFSFYYPYVRVVYHFFAFLA
                     RHLSWFKPLNLLIRAAFARYHAKVISGEDAVKILELDEDVTAVSEKNKQIVPFDHAYK
                     ILLQEPDFIAVMDCPCKKSLNAPEWTINSCISVGKKTSQFWLDRCGAKYHARKISQKE
                     AVDLVKKFREKGYVTQAFFKVATGGSTGIICNCHIDTCVSLQATRFARRFDPGLTMNA
                     DSGYSMHRDEALCRQCGACARICQFEAIVQSGDQQTYHKEICLGCGLCMEHCPEQAIR
                     IYRDPQKTVPLDMDIVKNEYASVQKIRDSSARSDAKRI"
     gene            9489..10781
                     /locus_tag="PLQ77_00045"
     CDS             9489..10781
                     /locus_tag="PLQ77_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HPL09099.1"
                     /translation="MQKEYDVIVAGTGTGGSTVAREMTRRGKKVLLLEAGGRWEWMGS
                     TMTIAMILKHCGLVRSREGRTVTFADNYGGLSNLAGGCAAPPPKSVFDPVGIDLAEEA
                     GEAKKDLWIQKQPDELVGRENLRLVEVANSLGYHWGKMDKFVDPKRCRASGDCMLGCR
                     TGAKWTARVYGDEAVSQGADLHLHTRITEVIVENNRVVGVKGTRRGVPVKYFGKFVVL
                     STGIDNAHILRRAGISRAGKGFCCDWLQFVGAEIPGMNTVGATPMSVGSLEFYESDGI
                     AIIPVFPNWSMYAATLAMAGPKSLLNFPRFWKLSGIMVKVRDETAGELFQGVSFSKKV
                     SEADRMKLDKGVDIIKKVFRKAGAKDSTILPLNPQGAHPSATCRIGDVVDRNLETEID
                     HLYCCDASVLPSSLGLPVMWTVVALGKRLGGHLAARIG"
     gene            complement(10916..11179)
                     /locus_tag="PLQ77_00050"
     CDS             complement(10916..11179)
                     /locus_tag="PLQ77_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HPL09100.1"
                     /translation="MTDPKKQKLRLDYPCLWTYKIIGRNPDEMQSAVHEIIRDRPCRV
                     SLSRQSETARYVCLNVELTVESESHRLVLYEELKAHRAIQVIL"
     gene            11339..11662
                     /locus_tag="PLQ77_00055"
     CDS             11339..11662
                     /locus_tag="PLQ77_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPL09101.1"
                     /translation="MLKKKRFLFLKNGFTLAALMLCAALIAQTAAFAQAAEKSAGEKL
                     PPAVRVNINKADQATLEKLLGIGPVKAKAIIDGRPYRNIEDVMNIKGIKGKTYDAIKE
                     HIVVK"
     gene            11700..11984
                     /locus_tag="PLQ77_00060"
     CDS             11700..11984
                     /locus_tag="PLQ77_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09102.1"
                     /translation="MKKMMVFVCAFLLFAAAAYAAGPRTYQVTGPVLDVKGDVIVVKK
                     GSDNWEVAKNAATRVAGDLKVGSRVTIEYTMTATKIDVKEDKKADAKKKK"
     gene            12260..13264
                     /gene="msrB"
                     /locus_tag="PLQ77_00065"
     CDS             12260..13264
                     /gene="msrB"
                     /locus_tag="PLQ77_00065"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPL09103.1"
                     /translation="MTQTEKEAVATLAGGCFWCVASDMKKLPGVTRVVSGYAGGSGKN
                     PTYGNYMAQGHLEAVQVHYDPGVVTYGQILHYFLKHIDPTDAGGQFADRGSGYRSAVF
                     YNNEAERKTAAGALQSLAASGKFGKPIVTELKLFTGFYPAEEYHQDYDAKNPLRYRAY
                     RSGSGREGFLREVWKKDRDNGPASAPPYRKPDDKILKKTLSPMQYDVTQKNATEPPFQ
                     NEYVRNKKEGIYVDVVSGEPLFSSRDKFDSGTGWPSFTRPLELSNITTKEDRSFFATR
                     TEVRSRHADSHLGHVFPDGPGPAGLRYCMNSAALRFIPREDLEKEGYGRYQKLFSQDK
                     "
     gene            complement(13302..14660)
                     /locus_tag="PLQ77_00070"
     CDS             complement(13302..14660)
                     /locus_tag="PLQ77_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPL09104.1"
                     /translation="MPSPHFDLVITGGTLVTMSAKMEIVENAVIGIADGRIARVGSHL
                     SLSADRVIDAGGGLILPGLINTHTHLPMVCFRGLADDLPLMAWLNHHIFPMEARFVNR
                     QMAYDGALLAIAEMILSGTTTCCDGYFFENQIAQAALDCGMRAVAAQGFADFATPDHP
                     QYEKMMAAAERFVERWLPHAPMITPAFFCHSPYTCSPRTLVNVKAAAREAGILYLTHL
                     LENRDEIGMIEKRYGRKPVQHLHHLGVLDDRTVAVHCNWTTPEDIELFADLGVRVSHN
                     PSSSMKLAAGIAPVPLMLEKGIAVGLGTDGAASNNDLDMFQEMDLSAKLHKAALLDPT
                     VMSAETVLKMATIDGARVLGLDRLIGSIEAGKQADIIILDLNQPHLTPLYNPYSQIVY
                     AARGADVKASIIGGKPVMENRRLLTIDLQDAMRNVGEIAAHIQAENQSTIRTSPVDVM
                     TP"
     gene            complement(14676..18644)
                     /locus_tag="PLQ77_00075"
     CDS             complement(14676..18644)
                     /locus_tag="PLQ77_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPL09105.1"
                     /translation="MLKYLILVVVLGVPAVLLAAAGWLFGTEAGGKKLLETVSSFTEV
                     NIRAETVEGNLADSLRLTGTVIEWPQGSLRIGKLRLSMRPQDLMAGHLRFSLLSADQV
                     SVFDNSPDKQPELVWPRAKGWITFVSGSIGRLAVRNLTYRRPNEEPVQITALAAAAVW
                     KNSSLSVSDLRLTSSFGVLEGSVLAGFHRPLLKAKLAATPAHPAAGVDSIRLDAAFGP
                     GKKPGEMKGSLQIHGLQNTRPVLRISSDIGMTPAGLTFAKILLRRGNRPGSIAGEGFL
                     AFKGPTPHAALKVQAENMDLSDELKVPAKFNGSLLLDGTGRQYQGRVVLSNTADTRHA
                     FRLAGDYSGNAEGATLSPIQATALGGSLNGRLKIGWTDGLKISGALSGKNLNPAKWNP
                     DWAGIVHFDLEGDLSAPAEAPPSGSFNLTLKESRLHGRQLTGDLRATFADNDIHIQRL
                     ALRGKGFQFAARGSVADQLDFSAEISDLARLIPHTSGSLTASGWARRRHDRFSGVVAA
                     RGRSLAADGWSIESGNFSASLEDREKSPFALTADFSRLRFRSLTTDTLRVRGGGTALS
                     HTLAAVMRRGPHETRLDLSGARREKIWKGKIIRLEGTDGVGPWRLSSPSALEISAERL
                     ALGPMEITGRGAEAFRASAVLSGNPLTGPVDLTWNDVDLARANAWTDHGIATGACGGI
                     IHATLLPSNRIDLALEATLSGTLSAQGQTVEVRQGKITLNANKRGTRLDVKFEPSDGG
                     WLQGSFFSSQPAKQSLPDTGQWDVRWRELDLKRFSASLPRRTKLEGRTSGEMEGKLLS
                     GGRFSCAGRAGLDHSKIHRRGQRGDVSIDFRRASAEWVWRKDTLACDVAVAMADHGEL
                     KGKIRLPLAARFGWTLNERGPLQGSLAGQVQEKGALGVLFPGLVQESRGTLDLDISVS
                     GTPADPLFEGRIRLSDAGGYLPSAGITLQDVRIEARPSKDGLVIDSFRAASGPGYVDG
                     SARIHLKGMQIAGFEGRIDGERFQTVHVPELHVQSSPRLTFWGTPEKISVRGEVLLPL
                     LEVNGSQSQGPAASSSDVIREGKTKSPGRKPSVGLDVQVKMALGDEAHFNASGIDAKL
                     GGEMDISFSDPERISGRGEIRVVKGRFRTYGVNLEIVRGHLFYAGGPVNQPALDILAL
                     RKIGDVKAGVAVSGSLPKPVIKLYSEPQMQDMDILAYIVLGHPLGADTKQAGLLATAA
                     SAVLTSQQAEDLLEQIQGYLGLASLEISSDVLEKSNRMGYRRLKVTPEGTGGSAQSAS
                     ETILVVGKYLTPQLYISYGRSLFSGGNLFFLRYDISKNWQVESQMGQESGVDLYYKLE
                     FH"
     gene            complement(18650..20455)
                     /locus_tag="PLQ77_00080"
     CDS             complement(18650..20455)
                     /locus_tag="PLQ77_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPL09106.1"
                     /translation="MTLSHPASTASPSAFRTWCCVFLLLGLLSATGPAVVVLAAGPVE
                     IVVEGVDGEARKNVLEALTLPPGLVKDGKADRLWLERFVRQAGRKVPAALEPFGYYSA
                     RAVVLIETPEADEYRLRVQIEPGQPAVVSGVSVTVAGSGASETTLAALAADFPVRKGD
                     ALSHPLYEKAKQALKSRAEELGYLDAAYTVHEIRVDEAGLSAVITLVLETGEKYFFST
                     TTIEGAPDYPDAFLRRYLAFQPGEAFSYTRMAESQLQLNNSERFRRVSVQADKEQSRE
                     GKVPVRVLLTPVPRITLRPGIGYGTDTGARFALRCRDLNMLHRGHELDSHLYISQRLQ
                     GLATSYIVPDGRDIRTFTSAQINLQQEEITTYDSRIIAMELARTHSFTPRRQGSAYIK
                     FQHEEFTIGSQEATSRYVLPGVRFSDNHYDNLIRPDRGFRYALDLRGTHQALGSTTRL
                     LQFIGEASHILPLPWRLSFLTSAKAGFTVFTDEFSDLPPSLRFFAGGDQSVRGYAYQS
                     LGPRDAEGNVKGGKHLLTGCVELQRDILEDWAVSVFYNAGNAFDTFSDLTLYQAGGFG
                     IHYHTPAGALNLYVARQIRVDDPEFRFHFTVGFEF"
     gene            complement(20482..20841)
                     /locus_tag="PLQ77_00085"
     CDS             complement(20482..20841)
                     /locus_tag="PLQ77_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09107.1"
                     /translation="MKKHLLIFLLIFLWMAAAWLPAEPAFADPPQDLSLSYDLKTQTL
                     SASITHKSTFTGFHYIKQVQIKKNQEPLETADYDSQAGKATFVYTYKVPAKPNDLLEV
                     TAVCNLQGKKTATLKVE"
     gene            20976..22286
                     /locus_tag="PLQ77_00090"
     CDS             20976..22286
                     /locus_tag="PLQ77_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPL09108.1"
                     /translation="MDALLLARLQFAFTIGFHFLFPAMTLGVALIILISETLHLATKD
                     DVYRRITDLLARLLGLIFVIGAATGIVMEFSFGTNWSRYSRTVGDIFGPILAAEGVIA
                     FFLESVFVGVLIFGRKKASPGVYWLSALLVFIGGHLSAFWILAANSWMQTPAGYEITS
                     AGKIVLTDFGAAVFNPSTVARFLHTVLATWVTGAVLVCGIAGYYVRKGLHGETARMML
                     KIGIILFAVTPLLQLGAAHGHAIQVIDFQPMKAAAMEGHFTTARGADVYVAGYVDEKN
                     EKTYGISIPKGLSFLYNFDFNSEIKGLREVPKEDWPPVNLVFQAYHIMVTLGMIFIGL
                     GLLGALLLWTGKLYTAKWYLWLLPFLIPLPHLAHETGWITAEVGRQPWIIQGLMRTAR
                     ASSVVVSKGELAASLTMFALVYLLLLVMFVMLFIRIVKQGPEAS"
     gene            22313..23323
                     /gene="cydB"
                     /locus_tag="PLQ77_00095"
     CDS             22313..23323
                     /gene="cydB"
                     /locus_tag="PLQ77_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPL09109.1"
                     /translation="MENIQNFQILWFVLIFVLLLGYTLLDGFDLGLAALLPFLGKTKE
                     EKDTLIASIGPVWDGNEVWLITGGGALFAAFPHAYATAFSGFYSAMMLVLLALIFRAV
                     SMEFRANDPARAGLWEKTLIGGSALAALLFGVALGNVIYGVPLDGRMEYAGSFFTLLR
                     PVPLLFGITGLAAVLLQGASWAVLKTEGRVQERSFEAARTLTWINAVLAVIYFVVLIY
                     AIPRVSGNALFYVAVAITFAGLIGLFFSLRKKNDAGPFWESSFSLIGLFLAAGAAQFP
                     NLITASNDPNFSLTIYNSSTSLYTLQVMAVIALAGMPIVIGYTLFVYRLFKGKAKADG
                     HY"
     gene            complement(23347..24480)
                     /locus_tag="PLQ77_00100"
     CDS             complement(23347..24480)
                     /locus_tag="PLQ77_00100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPL09110.1"
                     /translation="MKRDIAIDYLRSGVTLLVVAHHASLAYNTSSFYNADDYMKSSAP
                     VVDALRLLPLDFFVGWNDMFFMCLMFLISGLFIVPSLNRKGAGRFMTDRAKRLGVPFI
                     ISVFLLAPLAYFPSWLLSNTASQGGYLRGFFTNDGWASGPAWFIWVLLAYGAFVALAC
                     RFTPGLMKKLSWTAASPRNLVVVFLLASLAATLPLRLFVEPGHWAHIAGPLYFHAWRF
                     FLYFVWFLLGVALGGADPDRSLSRENLRPWPLWLFLGGITYLTHGTLEVNAAGLGITP
                     AWLMSVILTALYCLCCTFTGLAALGLARKFFHTARPVADHLTANAYGIYVFHYVFVIW
                     MQFFLLGQPLSPWIKFAAVFLTALAGSWLLTALLRKTPANQIL"
     gene            complement(24797..27334)
                     /locus_tag="PLQ77_00105"
     CDS             complement(24797..27334)
                     /locus_tag="PLQ77_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09111.1"
                     /translation="MKRDVGDMLTTLIRNQADREALETTAGAPGELPTLGVAPIRRGE
                     RHIAFDLSRIRVFRNLHTFVSLLAEEIIEQCAFGAADIMVRRKVDSRLTPELAAAGLD
                     WVTIYVRLDAAEGLPFHYRSFGDRLQMIFLTLQTERWGGALFPGYFGLQPSEASAPPA
                     LLFPFHLHDGGDPNGHYYLVEHSPSGHFLRITIEDAAHSRLQLKHIPHRVVDQPLRRS
                     FFTDIFSTAEKIHQGILHDVLNHRAEHVETSAHLPDFFAYLRQTGLPQLQQIRFTWPT
                     DDRQMMFLEKSDATALTESLDMLAREIQLLEDPAVLHLLEKGQTVEMLSGSFRIYFDV
                     SRWGACLNVSFDEIRERQDLADYLASMKALRAALALHPRALEGVRLFVIHHATAEALG
                     LLKAFADAQCDSLTAFFVQYAGIVPNDYLETLMSLPPQTFRFYGLQKMESRLRLAGSY
                     MLSRQFTPFSGLETMEQALIDEQPDFLDAMRMAAGHLFFKEADAARRANRLLLLVEDG
                     GYLAPIINRFCLEGKTLGEVFEHFHLPRPAEAFHMPLADWLAPVFPGSVEHTKNGYDY
                     LNEVAQAFGKLQYPALSIAVSDLKRGPEARACAVSILNAAENILHRLGLLLSRRTIVI
                     LGSAGAIGGFLRKELGRRLTKGCLFGVDVAAGNDAADGIQEAKTLDELGSGVLRNADM
                     FIGVTGLSLLQRGHLEEILLNGRQPAVFFISGSTKTVEFADAQHYLQSLRDTPNARIG
                     GRPVSADFKPLRDLQTGILQGYEIRLAFTDQPSCGKILYLLGEGMPLNFLYYGIPREI
                     VDEVMAQLFCVSCGLIRRLRAAAALPPALLAVDREIDANADLLPGKK"
     gene            complement(27352..28320)
                     /locus_tag="PLQ77_00110"
     CDS             complement(27352..28320)
                     /locus_tag="PLQ77_00110"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPL09112.1"
                     /translation="MDTMAAAVFDGKLNYVEDYSVPPVKPGWAKIQVKIAGICKTDME
                     ILKGYMGFKGVLGHEFVGIVMECDDPSWIGKRVVGEINAACGHCDWCRRDLGRHCPNR
                     STLGILNLDGCMAEYCTLPIVNLLALPEKLSDERAVLVEPLSAACEILEQMRPAGNER
                     AVILGDGRLGALCAWVLATVLPVVTVVGHHPEKLENLRWRNIRTVLHEKDVAPGADIV
                     VEATGSGAGIVTAMALCRPRGTIVLKSTVAVSGDVNLAPIVINEQTVLGSRCGRFADG
                     LKIMCEYPDMPLDRLITARYPLARVEEAFARATQSDALKVLLDIHP"
     gene            complement(28340..29368)
                     /locus_tag="PLQ77_00115"
     CDS             complement(28340..29368)
                     /locus_tag="PLQ77_00115"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPL09113.1"
                     /translation="MNPGAKKILLAGLAFAVVYGLLNSLSNIFLLPSAPIISMRPQIA
                     LPMVVGILVHPLAGFITGFAGNMFGDAVYGFGALKFWNWHIANGLMGWIPGLVGYGGI
                     RRIGTVREFGILQMSLVGASAAGVAAAVVLDVFFIHLLTFPYSFPSWILPAFLTDAVN
                     AFILVPVILLLARRIRLTLETRTILMITGLLLVSILATAGAITWAVLDDLVSHSAMVE
                     NFYIAAVVSVLLLVAGFIAAIVFVRKVTDPLADLAQAAQAVEQGNYDLPGLAGVSARA
                     DELGRLARIFEQMAGKVREREKTLQRQVDELKIIIDRKKQAQDVSEIVETEYFLALKE
                     KARKFRET"
     gene            complement(29365..30120)
                     /locus_tag="PLQ77_00120"
     CDS             complement(29365..30120)
                     /locus_tag="PLQ77_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPL09114.1"
                     /translation="MPEIIAIHSFRGGTGKTNTTANLACLLARKGRRVGVVDTDIQSP
                     GIHVLFNLDPKDAPHTLNDFLWGKCDIGRAVYDVSRILGAEDKEKLFLIPSSIRTGDI
                     ARVLHYGYDINLLKEGYQKAIEQFALDYLFIDTHPGLNEETLMSIAVADTLLILLRPD
                     QQDYQGTAVTVEVSRQLEVPRMFLIVNKVPTAFPGDALAAKVTAAYNCDVAGVLPHAD
                     EMMALGSSGLFCLMHPNTALGRAMEDILRRITA"
     gene            complement(30368..32575)
                     /locus_tag="PLQ77_00125"
     CDS             complement(30368..32575)
                     /locus_tag="PLQ77_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09115.1"
                     /translation="MISGSIFRKSKIICFLLGLMLCSPPGAAFAVDVQAIFPGWSVSG
                     QNSMHLDYYNSHGPDGAYLQEGWRPYNDLNIRFQNIQNPYNTIKGYASGAISGSPYRS
                     PQYGVILERFNINQENGEAPVPYRMDLGDFHGFQTPRVLQRSLKGAQLELQPGQFLGA
                     QNSLLLQGGGASRDFFDNRLADNLFGGASWMMNWSRTRASLNFAYNDQAPQSGTPDLR
                     QYVTGLAVNHNFNFLAQQLSLDAELNYLRGDNAQNGESFKDEGLGAFAQFQGRGKIPL
                     TYSLRYEEYDKAYIPRGGVVSAGRRTIDARAGWQSALNYSITGRGQYYRDGIGSAVTT
                     DTYVCGLGVSGAVPNPWVRGVNAGLDAYRQQAESDPAKTIIHALSANLTSQLPAGWTS
                     RLSANIQDTHVVYGISADNRTQQYGFDLGHGLALAGFSGRANAGIVYSILRQTGQNSD
                     DLGLNFSSLLSRGPLSFSLSYRHLNQDRQTGLVDNLTHGLNAACEYAYKRHQLRLEAD
                     YGYRKPQNSDESADTRIALTWTFSFDRPAAPVKAAPAEAPPAEAAKAAPAPPEIAPDV
                     QPPIRSLADLSPGMPMDRALAGLEKRNIKGGTREGRNLVFETVWFDDVSQRQRLVLTE
                     QMKKLERTAVIIDLNQVGELDNAYQLYERIRTRLNREFGAATTQEVGAFSPSLAQDLR
                     DGKFIRISDWKTPAGVLRLGIPRRLDGIVRIEAQHAQSFPMSGYVSWSVEEIQ"
     gene            complement(32568..33062)
                     /locus_tag="PLQ77_00130"
     CDS             complement(32568..33062)
                     /locus_tag="PLQ77_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09116.1"
                     /translation="MKKYLMVFILLLIALPGQAQEMRQWSPIRSKAHATAKLPPGAKP
                     VEPSQPLPSGTLARAAQEIAASWNSPDFKKLVSGNFYDQQRLTDAMNTETAKDARLKV
                     LNSHSASMLNQTIVPDPQGGHLRVSNVSVILETRAEFNDPKQGFISAPGTNEMVMEVV
                     EKID"
     gene            complement(33059..34033)
                     /locus_tag="PLQ77_00135"
     CDS             complement(33059..34033)
                     /locus_tag="PLQ77_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09117.1"
                     /translation="MGQQLMNRRSAFFTSALLMLLLLPCFAVFEAPAAGLGSENVLGR
                     DTQPVFSPEMKRQLQDRQRPAAQMTAEGKLSPGTWTVKILENRVISGHNFVNTAQPLY
                     NFTLNGVTYTFDRTQVADPALLLKTAVTIQTKGAGSLNLNDPETLSAVKQLQQMSNGT
                     LHIVSETPSPGPGPGPGPAPGPPVACMPAIYPLNGNVGTWIFLRLETLPASCAIDQNP
                     AGAPIIGIQNPHTSCSDAGSKVFFDVISGEPITPGAGFSTYTGVCGSGLRAVEASSIN
                     NQFIIQIGATRYQVTFSAGNGGLQPVPYSHFGRYNSITVYDASVRALP"
     gene            complement(34018..36939)
                     /locus_tag="PLQ77_00140"
     CDS             complement(34018..36939)
                     /locus_tag="PLQ77_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09118.1"
                     /translation="MKQLSVNIKILIALMLIIGSGALMMPPRSLAQQNQAPAQVKEES
                     PQEFVDRNLAAARKRLAAARSRYRQSRGPADLAQSPIDPSVILQSLPRPRKLKFSFRQ
                     PEKIEEIKPAPTVPGTWTFYPGYLSFSGAGNQSGFSANAATDELTSALLRLPRNLPLA
                     ATASIIGVNSQSHPVEKCCLEYKILKNNDGSFAGSQCIRTAQEKYISVQQNIDPRAWS
                     DRIGFRLTTSEGKILGNGAAVRFDQPGPAAVTATLTGQYKAEEFTPQNKQYCQVFDYA
                     TELIDNSKPARYRLNIQNFNVTDRWHNPGDKTVITGDWKIPQNKGEQKQKIDVEVIAV
                     KGLHLDGKDYTKTRSEADGYDLFARENEDFKGVYFRNDKKAPRLILQRAWPFDSVKID
                     GSRGGFREVLEPVSKWPTALTYAIEGGGPFAVSQDGDVQPLGSIGEARLNIQLGQHWS
                     LFRKLTANRWQAVLDNALADGAVSPGGSYSVALQVQGPADMAKYKVKWKGGQWKNPQA
                     KFEKMGNAWQAQNVVAVPGGTRMGTVLQLDVEVTVGDAGDEDVRLSWSRRLNVVPSVA
                     SLELTLAAKCRPASRDGVDLFFPNYHPDGNRFAALPIYRDDKGNELAPETIKRFLTGA
                     SLRLKSDTPAVAMADGNGGQTGRTAGEAWISAELGGIDLDPRGQFETAKGAMLASNQV
                     KVTANQLILSRGKASGGFTPYVLRVIGPAAMDQYQALFHFEGGSTGTPFIQGEDGGYS
                     AGLSAVAAVKKVEILKGGKTVAMLPFSDNVVVAQAGIRLMPVTVPGRVIDTIPLVDAG
                     SLTTQTDCRRQLRTQYPGEYGNLSDAALDEVCDTIREAEKKEIKEQRATQKEQKKILK
                     SLKNEGRQLMVFGDSTQLGAAVTGHFDPDRMHCRWRLDQGGSLKFEQAQSPITRVGGD
                     GACFNDVKGFQGNFDTEATATVELIYAHPAAGQGQFIPGGTQIMRADVDAKQVEEWVN
                     N"
     gene            complement(36936..39947)
                     /locus_tag="PLQ77_00145"
     CDS             complement(36936..39947)
                     /locus_tag="PLQ77_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09119.1"
                     /translation="MTKLFSRIFFILLLIAFAQFVPSGAAANAELEEIRRLETLLLTP
                     CDKPLAYMTELVRLGQTYQDQERQRLALYRGILDTHRLVLANFLTDNADKAGATAGPF
                     RARYDQLYQEADREGMLREASYEDVPEYMNMADMAVRVWQYDADQDRRYYGSEASVGR
                     WAKKQEDLGNKLFFVEAQRAGAEHLRKEMIRLKEDWKTARAEYFKTMEADPYKDHFSE
                     LDRRAAFMTDTLRREYESNRQALTIAAQSGSYEHCLGEIVESSTYTAFSENSGFSPPP
                     TIYQAGDAGTVEALPGMGFRMPKPAVFDRIKAAQTDIPEEFPLDLPLKFDPLPYIKTE
                     IECQKIKIKAEKDAIIAMGTYLTGLKGTGGLLVGEGFNVFHGVCEGFGSVGNILLGIG
                     YAQLHPIEAYEKLNKTKNDMVYLWESSDAKQALIEKGVGSIADYFRGIGSGFNLLLID
                     DPPDGVYDRQTMEDWRQRAEETQLARKMLTNTEMLVGSILADVATGVILSRGSQAAKL
                     AAEAADTAGDLSQAAARAKKLEEAAARAGKTLDDVPSVKTVEHLADDIRKNQDALMKL
                     ERDLSGKTRDIPYDPKMLRTPEDLLADASTVDTKGGMNAYVRLNEDLGMKVSNQPIIK
                     GDTPAAVLSRKSRDLDDAGRVYLQDITKDSSIGRLPQVKDRYYIVQDASGAAKTVRNL
                     AEIPDGASYKVVDVMEHIPSSMHADKLAGAQKLTADHVRAYEAFMRDMNAKGYVWTDN
                     KLSNFAFETVDEAKGVYRVVPLDTGGFYKVGDLPSGVNMSKADFARKMQRAYDKGVEA
                     NVEGAPGKMLAVSQNMVDEGIPESMLDQVDDIMGVRKINPQAEQMVNTWDSGYRMSSP
                     KNPQGQAYSHAGDYARESGKADDVLTREVSEAAEASQQARKLEYAKTRASLDAQKVRL
                     EQINSQLDAGAQKLVQADIVEKSAAATAARTSVNTAREGVQTVAGIQAAQERILCLEI
                     KEKILSGIQADWITEGWKNCQKLGLTFEGGR"
     gene            complement(39958..40590)
                     /locus_tag="PLQ77_00150"
     CDS             complement(39958..40590)
                     /locus_tag="PLQ77_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09120.1"
                     /translation="MLIKAPAESAFVELNSSYVPPMTIEGWACGTSGAEAGQKTVDLV
                     YRLRLAEQGFKNASAGLKNSYENRKKTLKAPAVFQGLSLPEVTATMAVSLNRFPKNIG
                     EFNADLNRLQKDLNQSLKEKPDRFFGVDISKRMLSESHLADRLRLETWQDDGAYQVWS
                     AIDCYSVADLQSMQSSISQALSAALTARDELIKTAAQVHDGQGAAFTLPR"
     gene            40962..42260
                     /locus_tag="PLQ77_00155"
     CDS             40962..42260
                     /locus_tag="PLQ77_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09121.1"
                     /translation="MERLWKGCLLCLAVLLFAGCTAVNHYLGFWQAKQEAREAFSREQ
                     SAELLMQLAPEDAYLLLGKLAFDREPAGPILLLALTDKFKKREIAARSPMQTPLDYYQ
                     IHLPEGVYEIYFFADLDENGYFEAHECVGRLPRPVTIAKQKVKDGLSVIGPDLKLNLA
                     APSKTDLVFEEKVRRHSYVYESLEDEFFDPRYGEIGLYDFKVFMAHTQQYIFSFETMN
                     PRKTQVLFVHGVAGTPRDFQYMVRGIDRKRYQPWFYFYPSGLPLKKLGANLANIIKYA
                     EANNPGRLRRLIIVAHSMGGLVALSTIQHLSADGVPPYLKGCISFNSPYGGVEAAASG
                     IKYAPAVVPSWHDVAAGSPFLNELYTEGKHKAIPFYLFFGYKTGDSSDGTITLQSQLN
                     RNIHLSAHKSYGFNASHVGILRDEQARQVFYRTLSDLDRD"
     gene            complement(42390..44618)
                     /locus_tag="PLQ77_00160"
     CDS             complement(42390..44618)
                     /locus_tag="PLQ77_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09122.1"
                     /translation="MRLQSIPRRGGGITGSVRRLACVFLMMFICLAPADVTAQTEGAL
                     QRAANAVLPDWQISGSNTFRLEYYDYQGDEAQAPYSYEGLTSYDEIGLNFSRRKNPYD
                     LWKGDIFGVINGSPYRSADYGLVPERIHLMREKGDIALPFRVELGDVYSYYTYRTLQT
                     SLKGVQVDLQPFAGSASRHSVLLSSGARLSSWRHLNFRDSYFNGGSYLIENPTFGRYS
                     LNIAYNTRQSDEARNILQRTQMVYSLTGQHTLQAKNQKLLLEGELGLFRGDHDGAADA
                     QSGQNKSDRGIFFQASGSGKHPFTYRLRYEEYGEDYQPTGASITPALRNLEGHAGWLF
                     ARGYQLRLRAQGRRNDLGRANPTDASLFGANLTGPLGSLDVFRQDAKNRTDSIDNLTH
                     NVNLNVNIPKIAGWTGRLGYQYLNFFNRLPDAGDSASHQFSLSADHLLDFLGCRGAVT
                     PGVVLRKINSDTGDSFDVSPTIAFRLTRGPHSLDYTLSRTDQRRYVTDGIDMITTTQA
                     LNYRFTLNNHSFGLELSSGYQNPDPGQTIDYYKLAAFWTYSFDKPAKTPGRGKAAEER
                     REIPEMPAKAFDLASFAPGAKMDEAEKQLIAKGIVNPVRQENFLLYETRVFDEIDLRQ
                     RLVLIQKNNALNRSAVIIDFAYLEQPDDIMQTFQRILSILLDRYGNPSSFYEKGETGG
                     RLLSDIRNGRFIRLTEWSRPGGVIRFGMPRRLDGQVRMEIQFAESFPPLSETLWSLEE
                     IK"
     gene            complement(44706..45218)
                     /locus_tag="PLQ77_00165"
     CDS             complement(44706..45218)
                     /locus_tag="PLQ77_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09123.1"
                     /translation="MKTKPISLPRILLAAVLAASISFTPAAAQEFRTINAIPTPGIIP
                     PGAAPVEEFRPIAKSAAADSVKKLMGAWNGGGIQQYLGDDFYDKSGLADAISTKVPKD
                     AAIRIMSVQGVQTLQQFQRNKDGGGHEMISEVSVTADTQIEYNDPTKGFVKFGGVNEY
                     ILRITEEAAQ"
     gene            complement(45232..46104)
                     /locus_tag="PLQ77_00170"
     CDS             complement(45232..46104)
                     /locus_tag="PLQ77_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09124.1"
                     /translation="MFQRMLMILNCLFLFVSFQNVLAEEIILGKDSRPLFPAELTERF
                     VGALPSSDKSSQAGKAQDALAESGAVLLGRGKTKITKIEVVRVIPVRGNNPLNQIEPV
                     NNATINGRLYFASVEELQKMMQGGGDSPAPGPGPSPPPPPPPPPPTPFAGCIGQSAPF
                     KSTSGFQTCLGKQMPTFSAGLFVDFAASACKVVGGDSGWNFLAFDIMVPLLSPDCTYT
                     DVAAFPDFAYAEFIPPTGSGFVRIPLKFDVEGRFYLQSKKAPYAGRKLVVFATVKKIS
                     NYKFELFISNVSPP"
     gene            complement(46108..48984)
                     /locus_tag="PLQ77_00175"
     CDS             complement(46108..48984)
                     /locus_tag="PLQ77_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09125.1"
                     /translation="MFRGKFYFVILPALVLWTFLLLQGFGNQVLAKDPEKGKKAEQLT
                     ISQPASFIAENLHNTIESLRNLEQTYEKNCGSLLLESISRGGNTQEIAGKLPKPKTLR
                     FNVAYPEEKLPQLSADALSDLRNPRYTPVLGGISYFEKAYPGYVAADSETAALLNLKN
                     RIPPTDIGFSPKIGFSILQDKFSLVSNFCGVMRRAVSGGEANVFSVPADVRGKLGVLV
                     SAAGGATVLDSGTVAFSSPGLPAVTISGKGGLQGINPELCQGRNEWTDYVNAGRDFTR
                     TIQFQSIKFKDFGIPGLDFNLPGAKAPFLDYFIPVNQSYNGVIASTKSLSTSAPERFM
                     LDIWADPYAIGRISPILVIDTGQEKEKPFEISDFPVTVHTDVKSPGPILPDGKGFLQY
                     AGGTGASVIAWNLGKLETEKRMVQHRFTVNSAAVEPAPEMRDGELAQGGEYSVKLMVQ
                     GEADMGGDYTVNWSSAGPAKWKESRTYFKKEGNVWSAVNTLIVDVLDKWPEGVGKKVD
                     FSASASYLPIIGSNPQKKDLLRMLQILSPPPRQDVFSFRGSAKLAMPVAEKLKLLVRY
                     GNRTPREMDEIDLFYPNHLAADVRLMPFLHLRNVKDPLALGDVKVPVQIEFKSTNPGA
                     AAVTTGGRLTSQSVYPMETEISAKISGYANISDPKENYKMLETAQAKLISNTIWVNVN
                     RLILSSTLQNGKTLYKLSASGAGDMSKYKALWKTAGIAGASETATQFTKDGDAFVATL
                     LSGSRVDHVRIVLDDLALAQLDAKSTLAQANIVLLPTRPPAAAVGKDTGDNSNDFKSM
                     TDCVSTVARWADMWGFETGGKPETYCRQKLQQQTQEAQNKRAQNRFAEQLKKRGLDLV
                     VFNDAMKVGAAVTGLSREFWERTWCRWRISSESKLYLEQDMTPVRMSSEMLGGCFNVV
                     KGIQEYFDPAAMIHVELSLDTDMPNYPLFVDPEKYVGSADEFRR"
     gene            complement(49004..51748)
                     /locus_tag="PLQ77_00180"
     CDS             complement(49004..51748)
                     /locus_tag="PLQ77_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09126.1"
                     /translation="MKNKFAGLMILFLVFFSFFAGISPAANQEGEKTDCANLKIQIND
                     IDKKIKRSCQDVQSLMDGLIDHWQSYYSSENQRLSIYELYNQKQWGILKDFVVSPDAN
                     AKAEPGQFYNLFVSLKNEARETGLLIDGTPPWSDKQRRAGIQISNSEFAFAPDAKSYF
                     YFGNPEKNEAGKAELSKKLNDIKSKWNEHKKKYDEFIKSDLDLANKDLASLSEKMAKA
                     KAGYFNNADQLSKAAYSGDYEHCLGKMAPASSSYTYIDGTEKLFIKGKKDTVEGLPGM
                     GLQFLQPTNLPVLARTNIPDCPFQLHLKRDGDKARAFVEEILKLELEKQERDTIELGI
                     DFVDETLSFAFDAAFHIVEATAPLGDAMIDIASHPIDSTVNGAKAVIEFGDKVLDRGY
                     LYKIKAEGGIGEAIGTALESSADKLGNLGSQLALRAVDSDRFTANPGDPPQKKLEKML
                     EQIESARQVNVGLKEMAKVTGEIAMLWKADAVIAKTGQAVSLAKAGVAGGKFESLANQ
                     LKALKAQNPALTAQVEAKTKSLEKIIEGQKDVNGKLEILQKKVVNEPVVKPFAGQPTK
                     AKATVQVSTAEGQNVSLTLGEQLGKGSTSTAFIDATNDSRIIRQTVLDKKAVAAAKLD
                     DFGRQALEKDVKSDSLRVAKREAVYTTPGDELGIEKAKKIEVVERVAEDAATTIKKQG
                     GILTEGQKMAYEQGLRDINSKGFAWVDNHSGNFAFEKLPGEDRWRVVVFDTGNIVKVT
                     GNTAEELAKNARAMQIRVNELGKTMEGKTFLEQAEIREKILGAYEKPLVDIKAMGIES
                     SDSIGMAAGDIRINTGNIAKMTDQELAVSLQGKTADAVLAAPAEDAIKARKKASPVPE
                     AAEIAESLEQAAGYTLMTNAASRLSDCAECVKYRKLLIAGEDPEKLKKMGNPCANLNH
                     "
     gene            complement(51813..52565)
                     /locus_tag="PLQ77_00185"
     CDS             complement(51813..52565)
                     /locus_tag="PLQ77_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09127.1"
                     /translation="MKINKVAEVMAGVLILASFAFPHAAFCENLSPDIIKITAPKESA
                     SGSYPHRSFRQFAVEGWACGDNLAPQIIHDIRTANLISEELVDKNQLLREGIQSLGLL
                     MEESRQQGLNSNQLSENASRLSESILSRTAAVDLTLTDYQAHRTISARSMEEIDRYYI
                     QKFHKEIEDFQKAAANGVNRLADTSAKFVGYETTLEQVMDNDETYVIVNCYTKSDLDV
                     FVSNQTQAYQTAIALFDEYQKLRRDVEKYRKR"
     gene            complement(52722..55340)
                     /gene="hrpB"
                     /locus_tag="PLQ77_00190"
     CDS             complement(52722..55340)
                     /gene="hrpB"
                     /locus_tag="PLQ77_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HPL09128.1"
                     /translation="MVMRHDCIDRIGCPPYTPAMQKNIKSGQILKPCPIDDILPELKT
                     AIRKHPAVVLQAPPGSGKTTRVPLALLDIIEPEQGRILMLEPRRIAAVSAARWMAGTL
                     GEEIGQTLGYSIRFDSRVSKKTRIEVMTEGILTRRILANPDLAGVAMVIFDEFHERSL
                     QADLALALCLDIRRALREDLKILVMSATLDGGPVSALLDGAAIISSEGKAYHVEERYC
                     GDNPDKHIAARVDEVVQRALRETPGDILVFLPGAGEIRTVGDAIAETLHGKAASGISL
                     HPLYGDLPFEEQERAILPASARKIVLATNIAETSLTIQGVGAVIDSGLTRRLEYDPGT
                     GMNRLVTVTVSQAAARQRQGRAGRLGPGVCYRLYSYHTFQSLIPFAPPEIAISDLSSL
                     VLDLAAWGVKDPALLSWLDVPPPSAWRAAKELLKELGALDANGSITPVGRAMARLPLH
                     PRLGRLLLRAADLDCPSFGADLAALLSERDIIRHSRSSFPHSAGGFDLSDRLGLLGQW
                     RQNKMLPKTADAFALRAVDRTSQQLRRLMGKTSEDAGVENGGMIPRLLLFAFPDRIAK
                     RREDDNSRYVLRQGRGVRLSAKIAPGGSPCLVAVVVDKGEKAEGMVHMAEPLTEELIR
                     EALQDQIETVRQVVWDKQEGRIIATREERLGTIILSAKPVNPPKEEVASVLCEAIRSR
                     TVMIAFSPEVLQFQARVRLMQQTFPEEKWPDISSETLISAPQDWLLPRLSGIRNAQQL
                     ADLNMLPALTERLTWEERRLLAERAPVTIAAPSGHNIPIDYVSGDIPVLAVKLQEMFG
                     LADTPVIAGGRIKLLLQLLSPARRPVSITRDLKQFWNTGYPQVKKELKGRYPRHPWPD
                     DPWNAVPTRKTKARNG"
     gene            55371..55643
                     /locus_tag="PLQ77_00195"
     CDS             55371..55643
                     /locus_tag="PLQ77_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09129.1"
                     /translation="MILLFLPDRLVGFPAVNAFMGFAEGAKPAFNLFDKPERGALLFF
                     AGMSAHGAFHADQRHFAIPVRDFIFRGFYGRLSSGPFIFLCGRFSF"
     gene            55806..58901
                     /locus_tag="PLQ77_00200"
     CDS             55806..58901
                     /locus_tag="PLQ77_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09130.1"
                     /translation="MKKMTCLLLICAFLACSQCVFAQDSAEKSSPKTTGEYLKKTAKD
                     QAASTAEDMAKKKVQEKVDQKAPQLINLAYEKYPVIAKFSGRKDFSELMKDYNKYSDA
                     YEKLTTVNSLLVKVAEGNVAGATYDATKEIFTKVFPYSKYIFSYLEAWDKILTSVSDA
                     VMENNLDYIRKEFMRDVLPKCKESCPLSEEQSFAGRIGRRMAEDDQGAYGLVAWYCKE
                     GGAAGSSKCGSICVSGPFDWSKRRSFENSGCEDYAVMSFVRSDQKIRQAVSAHYSILR
                     AIEDVGDMQKAMKNASDYVKKNLLVFEQEQGKIFAAQEALKEQQKKAAQEKIAMAEPP
                     TQCDEKCAESYRKAWYKTREISQEIQDKRSAINARVQEIQKENAARRNELKINVSSGN
                     LEELKTDPGNVSIDEDNVDYNGRQAQRLEKLVANAEKWLQSQQLEIRVYDGRISSLQG
                     LSGLYAQKQNLETQYGTGGTYRSSEKFTANYSLILENEIKGVELKRQELVDEITVVQN
                     NLSGYRSLQSKYKSAFEQGSKKARELLEKEVEPAYQEYAQADQELENIENERREYEQE
                     KLIQINVVMSKITAAKNQAELETLLPQVNKDMAEYNQLLQKQAQLMQKAADKSQKVEA
                     LSRSSRIQRAKGTLGYQSSSLSSRMVRPDLSGSKFSLAAYLPYIAEANRYVVTESARR
                     YGETLKKIPDEAAFIIMPDGDMKKLSMEVSSATGENAASSIKAQASTIARIKDFSGAY
                     SAYQSDLGSSLNSGNWKIDAVLSEEARAALNRLAAFWSEHFNPLMQKRSQKINEKITY
                     SQPKIGSGAQTLHGAVRLTAGDITDGKVPFQITASGWGISRALVRVAAAGKNLPLTIK
                     TSTAAEIPEAVYTAMVPVAAGKATHVSYTVGDWTHSVDLRAPAGHAVSPEDVGKIRAF
                     YDKFKQEYESRNDSRIVAMISDDWQAGDGSTLSDLQMNLRRTFRVFDEIRYNIQNLSV
                     TPGQDGRYNVSYDVTITSRIYKRNLKHEEKSSVQEEVTIGSSGKPKISKTLGGRFWTV
                     Q"
     gene            59080..62793
                     /locus_tag="PLQ77_00205"
     CDS             59080..62793
                     /locus_tag="PLQ77_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09131.1"
                     /translation="MKFCILIFILCLLAMPGLCKAASEEDVYDAMFNADASLFTTGYG
                     VFLDQVKDGQRFGASIDVFNKAAGFAATYSSVTTKAFATGDVSGAGEEAMLAVLSEIA
                     GWPSGQGLLKYVGLSTGVFQVALTAYSITKESFAQVRATKIALNIETLYGWIESDPVL
                     KPKKGRQLGDKSDPIPVNNESVEYMFRKVAQDERWRNLFKSYVSEELGETFPEPSTWD
                     WLSIAAVDGWKSAHTAEQAEIFKDKRQVETWIAGLLSQLNKYARAVESEVILRQTLAK
                     IEKASKKITPEFQKFVVGAEEAEKKLPEIDAYAKQVPTIIAAAKKNQNWDSLYDVRDK
                     IGYYARVYVRVLPDTGNIGKKKQATKAALKNSWVDVSKTLKDIPKTIKEDFTAQTVTP
                     QYKTSISLAEVSPDFIDIDEVAARLHKAKSLQDMEAEVILVKEDTEKKYRTFQENHNK
                     ENPAGDQHPAYNSRRDAMLQTLENQRKALEAQIASCDQYCRWLSGANPCGADGGKTWE
                     RCFNSASDAKRAWEKTAWKPLEDLETDKKNYNAKRELYAGNIFNQHEALLKAIGRLTN
                     EERARFADMEKLKKDALDTLGAVDQSVPIFYPETETKHSQVTAADTKQYLNSFRGQKT
                     DGYLGLPKMKFPKWGEDGMAVYEYVRRIERNVSNERQNFDRLSEKHSQAIQYIGKLKT
                     YLKEKDRYEQEIKEAKERIYALQLQSDPKAQVIPDIEKRIAEMKNVEKLALEIEAELN
                     NNLIAENQKQLAGIEDDLQYLDYLFKRIDYWRDAVRGNTLAGSVNATVVEVAVATENS
                     AGSNHHKYLAPSWQDLSLFDKAKAAAAANALLKDVERTGIAKWDPNGDTGLKVMLEKK
                     AAEIRNYCIDTPDYAIVNANVVYAARIARMAADVKAIPASTYGTNTYKEGLKNRTFDE
                     KLGAALGNGTAFAMNIEMKMNSNEQTVSVTLDKAKELAASHGNATIKTASSDMVKAIE
                     EHLSRYNAQVAQEAKQWEIDKAKAEAEQKLQNECLAKGGNYSNGECKTIPGGTGLGTG
                     GFPGQSGQAAGPGAIAAGGPAGINTGAPPAGRGAVMPQPPGSGAKAPAFRPDQTGPAK
                     GEPVGFSASAASRNMGGRSGMDDNLKDKVKDLYGRFKEAYEAKNASGVIYCLSDQWES
                     SDGGTLSDLQQNLRRTFRTFDQIQFRIENMQISNIAEDRFRVSYDAVITSRIYKRNLK
                     HEEKSSVQEEVTIGSSGKPKISKTLGGRFWYVK"
     gene            63000..64574
                     /locus_tag="PLQ77_00210"
     CDS             63000..64574
                     /locus_tag="PLQ77_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09132.1"
                     /translation="MCFFFLGNPFLLKAENEFHASNEFSYTYNDISGPGSSQSSLRQG
                     FSYLNLLNINDLGNYKGFDYNFNFGLKLTDDPTNDQQTASITNLQGRITNKVHTLNLG
                     DTFETFSQYALSSAVKGASYRFVKENSYIPEVSLVYGYAYSRWDNAFAIYDGPVKAVA
                     RQVMGARLKENLGQNFWAGLSYVQSQDNDRVNPTDSLYLNRAYTFDMEYKPIPGLTVS
                     GEASLSDQKEWTGNDGSETTRHGAAYRLTAVGDADPSRVTLEYERISPNFTTLVGSAT
                     PDREKAKAKWRYKYTKNISANLGFLWFRDNLDGQKTAGRTDYYKPEIGFTLKRFLGRA
                     YGTADIAYKLDVTSNNNTENYNHIVTLNYQDQFFGFLDSDTNLGYTLYDNKISVEERN
                     NEFTYNTSLSSRHTLGMVVLKPAVYLGGWTQRSELVVSTDQIYEYSAGFGLDVPDWKL
                     TSTIKAGQNRLDKSVGQDTDKTFASFNLNYRPPFLKKLNQGMLFFRAKYNDFSYTNAG
                     NNFRETSLTAGLILEI"
     gene            64599..66068
                     /locus_tag="PLQ77_00215"
     CDS             64599..66068
                     /locus_tag="PLQ77_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09133.1"
                     /translation="MKYKSKSLIMIMSFALVLLSFAGAQAKWWIFGESDEEVSISYLF
                     VNKSSFDETGAKITLFKEMLPDGKITISGKAVAGKGKIGSARVSLDGKETWKDANLSE
                     GGAFEYSFSPETGKTYSIFVEVSDTLGKTNDVAATARQLTLSERNIMSFIREALDKLI
                     AAYENHDAKTFMSLVSEDFAGDMTNLDRAIRKDFSSFDDIDLNYTLNNITSDSKGISV
                     SFSYRRLLMSTKTGQSVKDKGVTEFVFKMGDKSARIYSMKNPLIFGITGAPNVATGTV
                     ASTTNEPVIIADGRGNVALVPAQTFNQIVMDDSVRITNNPDGTSTIKTSDKTITVDKN
                     GQQTSGGSNPSETVEKGLRTIISLGHPPMGFSFVNGEVMTAAGDFMITGGGPGFGYGF
                     VEPSVTFVDLGAKSIGSVNEAPATGYANPAGMGVRFEEGHTYAFKLANGKYGLMEVKS
                     VTEEWIPGNPGPPPQPPAMRITMRFEYKYQPSGARQFLP"
     gene            66121..67398
                     /locus_tag="PLQ77_00220"
     CDS             66121..67398
                     /locus_tag="PLQ77_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09134.1"
                     /translation="MRRKIQFFGIKPVCLLIVMTIVLCFSGQCLAKGPAPKKAKAAQR
                     QEAPKAAVLPAPAVGKAEPEKKPAPPASFGVAAPRDAAAFFEEARKLAATDPVSAVAL
                     YQRGMMLKPDAWKERKELAALYEKQGQWNMALEEYEAVFRADKSAESYANRLRMLEKA
                     GYLRTAAAEARKAFAQYPDRPQFLLQAGELFHKAGDEAPATAALQEYLKLKPDDGRAL
                     MMLGSVYEKSKKPADALNAYLQAEKKMKGNQEAADAAKRLRAGNAVAGGLTIFLPEGW
                     SADQDGIFNLQSGERVTVSVKSAGSPAALALTSARDSMPKEPFSAENLKQMEKLKKMR
                     QELAKKDPESAKNMPSGMMPIHTQGDFAQLKGAKKAVLSTSETLQPGMESAVSVAVPS
                     GGKIYVFLWRASLPAADGEKTLSLLLGQSVWPL"
     gene            67411..70686
                     /locus_tag="PLQ77_00225"
     CDS             67411..70686
                     /locus_tag="PLQ77_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09135.1"
                     /translation="MMSRLLISNICRSLLLLFLCCFNPLAADAQEYQQAANALENLAS
                     EIRMNEAAVGQVLMEAMQSKHGDMQKWNDLDAKMAELGRSIDANLPEISRNAAIVLAQ
                     NPDQYVPSISLGRTFEGNLRMATDKRKQLGEQWKAENDKLMSINAKIAKVTELIGNAQ
                     AGSAIDAVMVLNPLPDDFWEFGGEATIAVLALYSGPVGWVALAGTAAAKGISMVTKSY
                     YNAKSLKERLKVYTELRDVLQEKKKIHEDNMKTLRDGEQEMRQIEQTLEKHEKKMTEY
                     KARVNSAMEALNNQYKSAFDARKQKLDEEVRKMADAPKPDLKPNGWVYGMDPIPPISS
                     GEYSGEVNAMISQMRSYAQAVEDGGDPDNFQIMVTDWHNRLHDRHKNIKKELDQKREA
                     HNQATQVFWRSYDAASAEYDRAWRIYVDCRDGSDRCYASLRAAGDRYNAAVRAAYAAL
                     RPFGQAMINPYRELIKIDQIHYRVRDAFYPFRERVDNAAKKNTRDFWNQSRLWETKFD
                     ETNAKVYDAVAPVPYWIDQWKSRAERLDDEIQSALYWGRNIADVRAELLSTAKDLRDL
                     HKTVQEAAKKYESANQERIQTANKAQSELNGILTRYGRLINYYWASNFSIDWLGDPME
                     FTPRAPDQEKNITSLEEYVKKKFTFYEPEHLKKSLKLDIPGIAAQYENKARELTFYTD
                     WVDTYRHRASTAVGALDRISVEKTGQYFYSDRGGTVPEVLSKEFGKPPWSTIAQDAEK
                     YVSKGDFASLPWGKFQSWENLGIWQKLYAGQTLLLNKLNKDAKNYVQARSNGWFMAVP
                     AQTMDPLIENWKKLREVSERFDKLAKPEHDKLAGGQEQAQKEAQPVFETWGKMPKYSR
                     DMVAEEYRRFSNAYNWLQDYIRSKFDATRTTLQPPNNSVAVNLDNLILGYAPAYEKWK
                     KQQEEADRRAEEQRRQWEAAEKARLEKEKKQAEEEAQRAARAIEEEKKRAQENLSLVK
                     DFYVRFKEAYEERNDSRVISMISDQWQAGDGTTLSDLQMTLRRTFKTFDEIRYNVQNL
                     SVTPLQGDRYSVSYDVTITSRIYKRNLRHEEKSSINEEVTIEKSGKAKISRTLGGRFW
                     YVQ"
     gene            70738..74277
                     /locus_tag="PLQ77_00230"
     CDS             70738..74277
                     /locus_tag="PLQ77_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09136.1"
                     /translation="MSVRRDWKLIYKRGRLFLLVILATLMLVNSAGAFSTDFEAGVDF
                     FGNFVEKGTNSGYGTVLKLDQPNLDPDKFKNFDWARLTMARFSTALDVVDKLNRGEYD
                     DAQLALALRAIQEIAGSESGGKLLGALGLSSLTVNVFVVSVKVYVASVRAVRESTRAV
                     QLESLYGHFESIANNPRLRNPGRVLGEGDPMPVTRASIDLLWRDIVDDENVRAQFYVY
                     VTQLLQRDWPEGPYWLGWYAAAHPPQKLPSAPNTVVQTITATPPPPFLQAQPVAGPTP
                     ELLASRRREMEGYIASLLGDLNQKRKVEEQLTQVRKSFIDLKDRIGRSRDLEAIMVKF
                     AQSLERLPAVEEFGKKCPGYIQNAENIKSQRSLEDLMEQSKGFAENVIQFIPAKGFYA
                     KDRARLLGLLKTCYIQAKEKIPVVAQKQKEEMEKPTPRPTDARKDETVQDGYAYYFKA
                     FVVPYAWSEEPDTAKRSVFVSLSKGKWDQADDVYARWTGKTYYGYDRGPGQTYVVHYS
                     DVLTKVTDLYNKPRNPLAKQYQAAYDSCDAAYRASFGPRDACYAAAKTEKDKQPCQDI
                     ESRASNAYSFCQESSGRPLRLQIEVLDAGFSRAKQAVGYLLEGDESLYRETKVAMDEA
                     FRSIVELQQEKKRQLETLRKDLAKFDGRLPLEEKPRVVEGLDRAIADLKKNSWPGVTK
                     EWMGGGGISSSSVVIDERALSLRERARPLPGSVDSWEAKIIEAEEGWNFAVRDWQAFY
                     PTVDLKKQDYDMITMWVDKTFSRDDFDRYDRAANRVPALVYSAKTKLGEYRTLAGTEF
                     HNISTDAAWLSSITAEFSKWVAQKQKEGIPYFSYSPGLHLLSSPYPHYLTKGEAALYA
                     AEAKQLKIYDFVQKYMPSSLAAFDADLLLGKIRLAEEDNFIIGFNTIAGNIAIYKSQI
                     SRANDLLASLKPDMGAVAYGEVLKEVSTVLPVTLERSNEAEDKAREKNAKSMKISYVP
                     LPWEGRLIFVDESKLNFPLGLAYLDLRKQLSVAIRKGQEYTIAERAKAAEKNGQQEQA
                     KKESPKTAEVAPSPAVSAGRDKEEIQAFYNRFKQAYEERNDSQVLSMISDQWQANDGA
                     TLSDLQMNLRRTFKMFDEVKYNIQNLSMILVESGNGRLRYLVSYDVTITSRMYKRNLR
                     HEEKSSINEEVTIEKSGKAKISRTLGGRFWYVQ"
     gene            74306..74812
                     /locus_tag="PLQ77_00235"
     CDS             74306..74812
                     /locus_tag="PLQ77_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPL09137.1"
                     /translation="MNIFKRILPVVLLLVMTVSGADAMAQETSKNLSVGKKASGKTSF
                     LETQKAVRETLDGMVEAYTGKNARQFMSFVAENYMGDDSLLERRIRRDFSKFLDMDIR
                     YTLNNVTTDSRNENISVAVSFTRSYTDVKTTKRINKNGSAAFIFRMVDGRPRLFNMKR
                     PYMFGVGK"
     gene            complement(74817..75887)
                     /locus_tag="PLQ77_00240"
     CDS             complement(74817..75887)
                     /locus_tag="PLQ77_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPL09138.1"
                     /translation="MFNSRGRYGNYPVLVSAILLVTLLMLCGCATKIRVNMLQPAQYH
                     EASLTKAVAVLPFTGPGGPEFAAEIEGVLAGIGIDDKPYFTLVDRASVDKIVSEMKFS
                     HSGLVDPKTAVKLGKMIGAQGIYTGVVTQNNHDDSFYYEKRSTCVHYEKRRDEKGRIY
                     QGACLQWRNYNVRCMKRVANFAVSPKLVDVATGRIVYSRNLSAVTASNGCEDTRPVQS
                     ESVLLEQAKASVKNQFRRDIAPFYVTREIRLIDSTDGIESNEAKDLLKRGLEYADKGR
                     MDSACELWGQARNLTPGSYALLYNLGVCAESRGDLDTALTLYRQSDRALGKPDDDITL
                     ALSRVGEAIKNRGKLQEALGVK"
     gene            complement(75900..77033)
                     /locus_tag="PLQ77_00245"
     CDS             complement(75900..77033)
                     /locus_tag="PLQ77_00245"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HPL09139.1"
                     /translation="MKTSPFLFCVLCLLCFSVPDAPAQSKCVHADGEALILNNDRPSA
                     KMEAVARAKWTAIEDVAGTEVKAGSLVQNFTLVEDVMKTKAGGVVRSYQVIDESVKDD
                     VLNVKINACVEPSQARNAVSQLGLNNSIALFIPARKPGRRGDEFEEKNILSETLIGKL
                     TEQGFNVVDVAPTHAVNARDVESAVRTGRTLSMRSLTYKFLSNLIIIGKVDYTISTKK
                     GEDIGYGLSMPFNHVTVRLNYRILSKNNNTGNMEILSTGVEQARGLANSVEDAAAQAM
                     QELAETLTPSLLDKVGQYIQGNTKKIAVQVAGVEDLDAVLKVKGILQNIVWVAGVEEK
                     EMGSFVVSYPENTLYLANSIQQKGSFKIVNFSPYSLTLEYLKQ"
     gene            complement(77133..78515)
                     /gene="mnmE"
                     /locus_tag="PLQ77_00250"
     CDS             complement(77133..78515)
                     /gene="mnmE"
                     /locus_tag="PLQ77_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPL09140.1"
                     /translation="MVTKTETIAAIATPSGTAGVGLIRVSGPLAPELAQRLFRPAHSN
                     CTWQSHHCYHGDIIAADGKTVLDEVLATLMRKPRSFTGEDVLEISCHGNPLILQNIME
                     QFLALGCRPARPGEFSERAYLNGRMDLSQAEALAAMICAQSAKALQLELSQLKGSLGN
                     SIDELRSLLIDGLANLEAAIDFTEDVSDKEIPAVPGQILQAAAGVELLLSTYRQARLI
                     TQGIRVVIVGKPNTGKSSLLNRLAGKKKAIVTDIPGTTRDLITETITLDGISIHLTDT
                     AGIRPPRDDIEKEGISLVRQQMEQADILLLLFDGSQPLTDEDYRILEQSKKRDCQILT
                     VVNKRDLAPAWDNRLPGHPLHGRRLLDLSAKFGDGVEDLKKALSDLAGGTDAPAEGGM
                     ISRLRHKLALEKALACLKAAQNCLATGQSSEFAAFELSEALNALDEITGKKISEDVLS
                     RIFSGFCIGK"
     gene            complement(78547..79539)
                     /gene="jag"
                     /locus_tag="PLQ77_00255"
     CDS             complement(78547..79539)
                     /gene="jag"
                     /locus_tag="PLQ77_00255"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPL09141.1"
                     /translation="MSAKILDIEEKNIDAAIERACREFGVGREKLNIEIISEGSNGFL
                     GIGAKKAKIRASIFSFDMDFSTEDSARQKERPQAKSDGKPEEKTDRPAERKPESRQEH
                     RQEKRHEAQPERRAARSAPAESRASAPRPARVKPAQPPAAPEQSPPPAVPRVEAVPAT
                     TPAALKARDLLAGILGRMTFECQVTAAETDETIILSIEGDDGGLLIGRRGQNLDALQY
                     LLNKSVHKSEAQRKMIVVDSEEYRKRREESLLAMAERIREKVKKTQKPLSLSHMNAHD
                     RRIIHLALQEDEELITKSRGEGEYRKVIVLPAKKGNSLRPGKNRSRKEPAGDSF"
     gene            complement(79557..81248)
                     /gene="yidC"
                     /locus_tag="PLQ77_00260"
     CDS             complement(79557..81248)
                     /gene="yidC"
                     /locus_tag="PLQ77_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPL09142.1"
                     /translation="MDKRTILAIVLSLAVLLVYQIFFVKPPVPSKGTVPQASTGQVRQ
                     DADAPSTPAPAATTASARPALKSAVGPQAPPRDITVETAHYTAVFSTRGAALKSLKLK
                     DYYRDCHECTDDIWPKIKGLVTGTKAPLKPKTPEFVELVDVREGMPYPLAVTFPESGV
                     DVAPDEVFDAAGNRMDLTGTQQKQQLVFSRSQGGVKIEKIYSFDPSGYTLGLDVKVHN
                     LTGAPITQIPQLNWYQYVDPQKIDDSYGHEGPIVSVGGSIELEEVKKLQSQSVQGPNV
                     LWGAFESKYFIASFIPENPSLTNAVLTRDAANMVGVGMKGRKEIIPGGQNSVFSYSLY
                     LGPKQYDLLKNLGVGLENAIDFGWFKWLAIPALYILNFLYGFVLNYGIAIIILTTIIK
                     IVFWPLGNMSYKSMNEMKKLQPKIEALKQKYKNDQTKIGQETMALYREHKVNPFSGCL
                     PILIQIPVFFGLYKALMYSIELRHSPFFFWIQDLSAKDPYYITPIIMGATQFITQKMT
                     PAMGDPTQQKIMLFMPVIFTFFFLNFPSGLVIYWLWNNILQIGQQYYINKKLATG"
     gene            complement(81272..81499)
                     /gene="yidD"
                     /locus_tag="PLQ77_00265"
     CDS             complement(81272..81499)
                     /gene="yidD"
                     /locus_tag="PLQ77_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPL09143.1"
                     /translation="MFRKTLVNFFVFLIRLYQACISPFFFASCCRFTPSCSQYAIDAI
                     RLHGPAKGVWLGARRILRCHPLHPGGYDPVQ"
     gene            complement(81503..81850)
                     /gene="rnpA"
                     /locus_tag="PLQ77_00270"
     CDS             complement(81503..81850)
                     /gene="rnpA"
                     /locus_tag="PLQ77_00270"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPL09144.1"
                     /translation="MKAQSFRRAERVTSKSRYRTIYEQGVRRSSRFFTIITCGNPKGA
                     KRLGITVTKKTGNAVFRNRMKRLLREFFRRNKELFPAGHDVVVMVKKNTPPSNYQETA
                     RELTELFLRKAGK"
     gene            complement(81895..82032)
                     /gene="rpmH"
                     /locus_tag="PLQ77_00275"
     CDS             complement(81895..82032)
                     /gene="rpmH"
                     /locus_tag="PLQ77_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPL09145.1"
                     /translation="MKKTLTNKSNTKRKRTHGFLVRMATKTGRQVLSRRRAKGRKKLT
                     V"
     gene            82428..84299
                     /locus_tag="PLQ77_00280"
     CDS             82428..84299
                     /locus_tag="PLQ77_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL09146.1"
                     /translation="MALNPLLDSRDQRFVLFEALEADKLNRYERFADFDRDTYEATLD
                     LAEQIAVEQVYPANAQADKTGAIYDPAAKTVKVPEGFHAAMKSYREAGFSGLSNSPEF
                     GGTGMPETVYRAAMEYFCAAGVAFSSYDTLKVGACNLIIHHGSEELKKIYVEKMVAGQ
                     WGGTMCLTEPSAGSDVGALKTRAVRQADGTYKITGQKIFITAGDNDLYENIIHPVLAR
                     IDGDPPGTPGISIFIVPKFHVNPDGTSGKRNDVATTGIEHKMGIKGSATCSLSFGDND
                     QCVGWLLGQERQGMKIMFQMMNEARLDVSQQGLGTASSAYMHAVTYAKNRLQGQDPAK
                     KGDFTSVPIIRHPDVKRMLIWMKAQVEAMRMLTLLAAYSADMANVEKGTPEGREAQGL
                     LDFLIPLCKAGNTDLAWLVTSEAMQVFGGYGYCSEYPIEQLARDCKILAIYEGTNGIQ
                     SMDLTMRKLLMNPDMFNFKVFKKRIALSCEKAGGIVEDKYIAAMKNAVDAMDATVAKL
                     TEWRDRKRILDILAVAQPLQQAMRMVAHAWMHLWSMTVAIPKLKAIAGDIKGEKAASL
                     AKDNAEAAFYMGKVLSGQFYLGAELKHLTGYLDYVTAGEPAAVNECFENIFTGALPQ"
BASE COUNT        18770 a        22951 c        24315 g        18796 t
ORIGIN      
        1 ggacggcgcg gcgcacatga gctgggtcat ggtcaacaag cgcgaccaca tgaacgcgac
       61 ccgggatctg cacaactgga tcgaagtcta tgaaatgttc gccaaactct ataagtttat
      121 tccgtggtaa aaaactgcgg ggaacgggct gaagccgttc cctacttggg cttttctacc
      181 tgaatgaagg ggaccgcgcc gcccgtgacg ttcggcagtt ttccgtccca tttttcaatg
      241 gctttgatct gggcttcgat ctggcgcagc tggatcagct ccggcgagat cacctgccgc
      301 tgcagccgga gcgcttcggc ttcggccttg gccgaggtga ccttctgttc ggcttccacc
      361 ttgattcttt ccaaatcgcg cttggccctg agcgcgtttt gctcggcggt ctgcttggat
      421 tcgatcgccc ggttgaattc cgccgagaat tcaaaatccg tcacggaaag ctcgacgatg
      481 ttgatcccga agacgtgcag cctctcccgc agataattgc gcacttccgt tttgagggtt
      541 tcccgcttgg agatcagctc ctccgccgtg taacgggccg cggccgcctt aaagctttcc
      601 ttgacggccg ggtcgatgac gcgttcattg aaattcaggc cgatgttggc gtacatctcg
      661 ccggttcggg ccgggtccag gctgtagttg agcaccatcg tcgtttgaac ggtttgcaga
      721 tcgcggcttg ccgcttcggt cttggtggtg gtcttctgca cccggacgct catgcggacc
      781 acctcctcgg caaaggggat tttgaatcgt gctcccggct caaccacccg gttgacctgc
      841 ccgaagcgca gcacgatgcc gcggtcgccc gcctcgatgc tgaaatacgt ggacagcaaa
      901 agaaggatgg cggcaaaacc aatgacggcg ccgatgatca cctttcccgg aattttttgt
      961 gcatccatgg ttcaacctcc atacggtgaa ttttctgata tttatttgct ttcgcccagc
     1021 gccggtccgt tctcagtgcg ttttggcaaa agcgccgcgc cgcagccatt tttccgcgcc
     1081 gaccacgacg taaacgataa tggaaatcgc aatgatcatc agccagtccc ggccggaaat
     1141 cggctcgctc tgaaaaacgc ggttcatggc cggggtgtag gtgtagagca gctgaagaac
     1201 gatcatcgcg gcgctgcccg ccaaaaccca gggattggaa aaaaagccga tccggaacat
     1261 cgatttggtc aacgaccggc agttaaacag ataggccagt tcaatcatga cgaaaagatt
     1321 caccgccacg gtgcgggctt ccgccagaga atagccctgc tgcttctgcc agacgaatat
     1381 gccgaagacg gcggcgagca tgatggccgt taccagcaga attctggtaa tgaccgtcgc
     1441 gttgagaatg gggctctggg gatcgcgcgg cgggtaattc attacatccg attcgcccgg
     1501 ttcgaaagcc agcatcagac ccaagagcac ggctgtggtc atgttgatcc agagaatctg
     1561 cacgggcaga atcggcagcg tgactccggt tacaatggct gccaggatga ccagaccttc
     1621 gccgccgttg gtcggcagcg tccaggtaat gaacttgacc aggttgtcga aaatccgacg
     1681 gccttcttcc accgcgcgcg taatgctggc aaaattatcg tccgtcagca ccatgtcggc
     1741 ggcttccttg gaaacgtccg tgccggtaat ccccatggcg atgccgatgt ttgcctgctt
     1801 gagagcggga gcgtcattga cgccgtcgcc ggtcatggcg acgatatggc tgcgcgcctg
     1861 aagcgcttcc accagccgca gtttctgttc cggagcgacg cgggcaaaaa cggaggtgtc
     1921 ctccgccgcc gcgatgagtt cttcatccgt cagcttttcc agttctttgc cggtcagggt
     1981 tcgcggtgcg gcctcgtctt tgcccttcag gcttccgatg ttcagctgcc gggcgatttc
     2041 cgttgccgtg accgcatggt ctcccgtaat catcttgacg gcgattccgg cccgacggca
     2101 ggatcggacg gcctcgatgg cttcctttcg gggaggatcg atcattccct ggagccccag
     2161 aaacgtcaga ccggacaggc aatcttcaac ctccagatga tccttgtccg tctctttgca
     2221 ggccagcgcc agcacgcgca ggccctcgct tgccatggcg tccacccggg ccggcaggtt
     2281 ctctttgtcg aaagcggttt taccgccctc gcttgccatg gcgtcggcgc aggcctccag
     2341 aattttttcc accgcgcctt tgatgtagag gatgtttttg ccgcctgctg tggaacggtg
     2401 cagcgtggcc atgaactgcc gctccgattc aaaggggatt tcgtcaagac ggggcatgtc
     2461 ggcgccggtt ttctccggtg tcaggccggc ctttcccgcc gcggtgatta aagcaccttc
     2521 cgtggggtcg ccttcgatgc gccacgcatc ttcttcctga atcaggcgag aatcgttgca
     2581 cagaagcccc gccgtcagcg tctggcgcaa tcccgggggc aaatcgccgg ttaattcctt
     2641 cccgttcgcc gaaagatggc cttccggcgc gtaaccgttt ccttccacgt cgtaggaagc
     2701 cccgcccgtg aaaatctttt ttacggtcat ctggttttcc gtcagcgttc cggttttatc
     2761 cgagcagatg accgtagtgc tgcccagggt ttccacggcg ggcagcttgc ggatgatggc
     2821 cctcttcttc gccattcggt tgacgccgat ggccagcgta atcgttacgg cggccggcag
     2881 tccttcggga atggctccga cggccagggc gaccgccgcc ataaacatat ccacggcgct
     2941 ttcaccgcgc cacatgccca cggcaaacgt cgccgccgcg agaaccagaa tcgcgatcag
     3001 aagaattttg ctgaacgagg cgatttttat cgttaagggg gtggaaagat cggcggccga
     3061 ggagatcagc tcggaaatgc ggccggtctc cgtcttgtcg ccggttgcca cgacaaccgc
     3121 ccgcgcctgt ccgtacgtga ccagcgtgcc ggcataggcc atattgcggc ggtcggccag
     3181 gatggtttcc ggatcaaggg tctcttcttt tttgatgacg ggcacggatt ccccagtgag
     3241 ggcggattca tcgacctgca gatcccgaca gcgcaggatt cggacatcgg cgggcacctt
     3301 gttgccggac tgaatctgga cgatgtcgcc gatcaccaga ttcaccgcgt ccatttcctg
     3361 ccaccggccg tcgcgcagca cggtggtttg cgtcgtcatc atttttttga gcgactcaat
     3421 ggcgctctgg gccttggatt cctgcacaaa accgatgacg gcattgatga tcaccacgcc
     3481 cagaatgacc gacgaatcca cccattcctg aaggaacagg gtgacggccc cggcggccat
     3541 cagaatgtaa atcagcggct gatgaaactg cagcagaaac cgcatcagcg gtccctgttg
     3601 cttttttacg gtgacggcgt tggggccgta ttttttcagc cggtgtccgg cttcaaaagg
     3661 ggacaggcct ttttccggat cggtgttgag caaggtggtc acttcgcttc cggggcagtg
     3721 gtgccagtgt ttgcagataa tggattccat ccaagacctc cgggtggttg gccctgcttc
     3781 ccccgggaaa gggaaggggc ggtgaagggg tatcaggcgg cgacggtgaa cggcaattta
     3841 aaaacggcgc caccttatcg caatccgttg aagattgtca agacaaaccg gctgaagctt
     3901 cagcggcggc cgcggccgca ggctttgttt ttgcggactg cgggttttcc gcggggaata
     3961 aaattgtcat tgatattttt gaaaagcttt tgcatgatgt cgtctcgtaa aaagaaatct
     4021 ctggaaaggg aaagtttgcg cttgtccgga aaaacactca ttctcgggta tgacgccgtt
     4081 tcgtctctgg ggacggatct ggaaatccag tggcggcggg ccgccgccgg cgaaagcgga
     4141 atcggccctc tgacgcgttt cccctacggc gatgattttc ccgttcgcgt cgcgggcgag
     4201 gtcgaggacg tggatgtccg gccctatcct tttttgcagc cgcgcgaaat ggcgcactgg
     4261 acgtcgccga tcttcaaata cgcgctcctg gtcgtccacc gggcgctggc gaagagcggc
     4321 atcgacatca cgcccgatgt cgcgccgcgc gtggccgtca ccttcagttc ggccgtcggc
     4381 ggacaggacg cggtgctgaa ggcggaccgc cagatgatcg ccgaaaacaa gctgccgcat
     4441 ccttttgcca atcccaattc ctgcatcaac atggtgggcg gcaaggtgtc catcctgacg
     4501 ggcgccaccg gtccgatctg cgccacgatt acggcgtgcg ccaccggcgt cacgtccatg
     4561 atcatcggcg gcatgatgat tcaacagaat ctggcggacg tggtgatcgc cggggccgtc
     4621 gattttccgc tggtcgagcc gatcctggcc ggctttgcga cgatgaacgg cgcgtatcgt
     4681 ccgaaagagg gccggcccca cgaagcgccg gagaaagtaa gccggccgtt ttcggccaac
     4741 cgccggggat ttgtcgtttc cgagggggcg ggcgcggtca ttctggcgtc accggatttt
     4801 gcgcgggcgc acgggcttgc ggcgcgcatc gaaatcgccg ggttcggcat gacgtccgac
     4861 gccggccatt ttgtggcgcc taatccggcg acggtcgagc gctgcatagc cgtcgcaatc
     4921 gcggatgcgg gactcacgtc cgcggaggta gacgccgtca acgcccatgc gacatcgacc
     4981 agaatcggcg acaaggtgga agcggacgcg ctcactcaaa tcttcggcgc aaaggcgccg
     5041 cccacttcgg ccaacaagtc gcagctcggc cacgccatgg gcgcgtcttc cgccattgaa
     5101 acgattctgg cgatcgaggg catgctgcgc gacacagtcc tgccaaccgt caactacgaa
     5161 ccggacccgg ccattcatct cgactgtgtg cccgggaccg ccagaaaact ctgtcaggag
     5221 tttgtgctca aaaacgcgtt cggcttcggc gggtgcaatt cctgcctgct tttgcgccgg
     5281 atcgcataag gccctgaaag gcaaaggatg aaacctccgc aaaaccgaag agtctttatc
     5341 gtcgggtatg gagcggccac gccgctgggg agcacgtttg ccaaaacctg ggagcgcgcc
     5401 gttcagggcg atgcgggatt ccgcaaggtc acccgctgca aggtggaatc ggcctgcgac
     5461 atcgtgggag agattccgga ctggtctccc cggaacctgg atttctccga cgccagggaa
     5521 gtctataact ggaacgcggc gttcgttctc ctgaccatgg cggtctgcaa ggaagcgctt
     5581 caaaacgcgg gaatcgtgat ggatgaaacc attgcccccc gaatggcctg tctgatcgga
     5641 tcggcgctca acgggacgga tgcgtttcgc gcggccatgc acgacctgga acatcgcggg
     5701 cctttgcggg taagtcctta cctgctgccg aacctgtgcg ccaatctgcc gtcgggaaag
     5761 gcgggtatgc tcctgaaatt caccggaccc atcttttcgc cgcagggcgc ctgcgcctcg
     5821 ggcaaccatg ccatcggcat cggcgcgcgc atgatccgcg acggggactg cgattttgtt
     5881 ctggcgggcg gggcggatgc gccgattctt ccggagctca tccacggctt taccaacatg
     5941 aacgctacga tcaaggtgca cgccggcgac cgcgcggccg cggatcccgc gcaggcctcc
     6001 cgtccgttca gcatcgaccg ccgtggattt gtcctttcgg aaggagcggg cgtcgtcgtg
     6061 ctggccgccg aggaggttat ccgggcgcac gggcttgaac cgcgggcgga ggtgctgggc
     6121 gtgggctgga cgtccgacgc ctaccattac accagcccca atccggcaac cattgtccgg
     6181 gccctgcggg aaaccatcgc ggacgcgggg ctccagcctg ctgatgtcca gtatgtcaac
     6241 gcgcacggca cctccaccgc caaaggcgac cggacggaaa ttcaatgtct gcgcgaagtc
     6301 ttcggcggcc tgatggaaat gattcccgtc tcctccaaca aatcccagct tggccacacg
     6361 ctgggagcga cggccgccat tgaagcggcc ctggccgtgg aaggcatgcg gcgggggatt
     6421 ctgctgccga ccatcaacca cctgccggat ccggaatttg ccgatatcga tgtcgtgccg
     6481 aacacggccc gcaaacaaac ccatgaaatc gtcctgtcca atgcgtttgg attcggcggg
     6541 acgaactgct gcgtgatctt ccggggagtg tgatatgaaa cccaagccgt ttttgcctga
     6601 aacctgcaag atggatgaac gattcgtccg cgaccggacc acgggactgg tctggcaccg
     6661 gaccccgtac cgggtgctgt atgcggacac ggaccgctcg caggtcgttt accactccaa
     6721 ctatctgcgc tatttcgaag tcgggcgcac ctcgctgatg cgggacctgg cctatcccta
     6781 cagggaaatt gaagaaagcg ggtatgtgta tccgatttac gatctgggga tttcgtttta
     6841 tcagcctctg gtttacgacg acgtcatgca cattcacacc cggccggtca acctggagcg
     6901 cgtgaggctc cagttcgatt atgtcgtcac ccgcgcggat gcggggcgcc tcgtctgcac
     6961 gggattcacc agacactgcg cggccaacgc cgccggccgc cccgtggccg tggatccgaa
     7021 gacgattcgc ctgtggcaga cctttcccga tccggtatga aacagatctt ttccttcaag
     7081 cttccgatcc cgccgtacct gcgcgaccat tgcgtggagg ggcaggccgt tttccccgcc
     7141 gtggagtccc tcgcgctcct ggcccgcgag gtgcaaaacc gccgccccgg cgctcttgtg
     7201 aaccgcctgg cggacgcgga ttttcccaaa atgctggcgc tgccggagga ggcggatgag
     7261 ctgcctgtgc ggattgaaat tgaggaaagg gagaacggga ttcatgcgtc cctgcagacg
     7321 acggtgagcg ccaaaaccgc ctccattcga agggcgctgg tgcatgcgcg ggtgaccttt
     7381 gtccggaagg atgctccgcc cgaagcgccc atgccttttc aggcgtcagg aacgacggga
     7441 gacgacagca ttccagtcgc ggcggattcg gtttaccgaa aatggattcc ctttggtccg
     7501 tcctaccgga atcttgtcgg aactctggcc gtttcgcggg aaggcgtgtc cgcggaaatt
     7561 tcaggaggga gcggcgaagc ggacgacacg ccgctgggat ctccttttgc gccggatgcg
     7621 gcaatgcagg ccgcctgcgt ctggggacag cggtttgccg gcattgcggc ctttccgacc
     7681 ggttttgacc acagaatcat ttaccggccg gtgaaaaacg gcgaaacctg ctttgccagg
     7741 gtgatcccgg tgaatggaaa ccgcggattc ctggtgtttg acgtgtggat ctttgaccgg
     7801 agggaaatgc tttgtgaaag cattcgcggt ttgagaatga gcgatatttt ccgggggcgc
     7861 ctgaagacgc cgcgccggat cggggaaggg gaatgatcaa cgtcatttgg atttgttgcg
     7921 cggcgtatct ggccggctcc gtcaattttg cgattctgtt tttcaaaatt gcaggccgcg
     7981 ccgatccgcg cctgagtttc agcggcaatg ccggaacgac gaatgtctat cgtctggcgg
     8041 gcatgccctg ggcggtgctg gtttttgccc tggacatcgg ccgggcgatg gccgtggcgg
     8101 cactggccgc ttattttctt gatggcgggt ggctgatctg ggccgggttt tttctggttc
     8161 tcggcaacag ctttccctgc tttcacggat tccggggcgg caagggcgtg gccaactacc
     8221 tggggtttac ggttctgacc gttcccgtcg ccgcgctttc ggccgcgtgg ctctgggttc
     8281 tggcttactg gctgaagcgg gcgccgttta tcgcttcttt tttcatgacg gccgcgcttt
     8341 cggcggggca cgtcgtttct tccagcggag actggcttgc cgcggccggc gccgccgcga
     8401 tctttgcgct gattgtcttc aatcaccgga agaacattcg ggagtattga aaaaaacttc
     8461 ttttggcgag gcaaaccaaa ggaagcggcc gatgctgcgg gagacttctc ttgatgggat
     8521 ttccaatctt ttataaaatg atttccgaag gggagcaggc atgcgggcat caacgaaagc
     8581 tctgatggcg aagcacggct ggcggatcga ccgggccatc cacaactatc tttatttctc
     8641 gttttattat ccttacgtaa gggttgtcta tcattttttt gccttcctgg ccaggcatct
     8701 gtcctggttt aaacccctca acctgctgat cagggcggct ttcgcccgtt accatgccaa
     8761 ggttatatcg ggagaagatg ccgtaaaaat tcttgaactc gatgaagacg tcacggcggt
     8821 ttctgaaaaa aacaagcaga tcgttccctt cgatcatgcg tacaaaattc ttcttcagga
     8881 gccggatttc attgccgtca tggattgtcc ctgcaaaaaa tctctgaacg cgccggaatg
     8941 gaccataaac tcctgcatca gcgtggggaa aaagacctcg cagttctggc tggaccgttg
     9001 cggagcgaaa tatcatgcaa ggaaaatttc tcaaaaagag gccgtcgacc tcgtcaaaaa
     9061 gttcagggaa aaagggtatg tcacgcaggc cttcttcaag gtggcgacgg ggggcagcac
     9121 ggggattatc tgcaactgtc acatcgacac ctgcgtgagc cttcaggcga cccgcttcgc
     9181 ccggcgcttc gatccgggcc tgacgatgaa cgccgactcg ggctactcca tgcaccggga
     9241 tgaggctctc tgccggcaat gcggcgcctg cgcccgaatc tgccagttcg aagcgattgt
     9301 gcagagcggg gatcaacaaa cctatcataa agaaatctgc ctgggatgcg gtctgtgcat
     9361 ggagcattgt cccgagcagg cgatccgcat ctatcgggac ccccaaaaga cggttcccct
     9421 ggatatggat atcgtgaaaa atgaatatgc gtctgttcaa aaaatccgtg attcatcagc
     9481 gaggagtgat gcaaaaagaa tatgatgtga tcgtcgccgg gaccgggacg ggcggttcaa
     9541 ccgtcgcgcg ggaaatgacc cgtcgcggaa aaaaagtctt gctgctcgag gcgggcgggc
     9601 gatgggaatg gatgggcagc accatgacca tcgccatgat tctaaagcat tgcgggctgg
     9661 tgcgcagcag ggaaggccgc acggttacct ttgccgacaa ttacggcggt ttgtcgaacc
     9721 tggcgggcgg atgcgcggcg cctccgccca aaagtgtttt cgatccggtg ggaatcgacc
     9781 ttgccgagga ggccggggag gcgaagaagg atctgtggat acaaaagcag cctgacgagc
     9841 ttgtgggcag ggagaatctg cgcctcgtgg aagtcgccaa cagcctgggg taccactggg
     9901 gaaaaatgga caaattcgtc gatccgaagc ggtgcagggc cagcggggac tgcatgctgg
     9961 gctgcaggac cggcgccaag tggaccgctc gcgtctatgg cgacgaggcg gtcagtcagg
    10021 gcgcggacct ccatctccat accaggatca cggaagttat cgttgaaaac aatcgggtcg
    10081 tcggcgtaaa gggcacgcga cggggtgtcc ccgtcaaata tttcggaaaa ttcgttgtgc
    10141 tctccacggg gatcgacaat gctcatatac tccgccgtgc cgggatttct cgcgccggaa
    10201 agggtttctg ctgcgactgg cttcaattcg tgggggcgga gattcccggc atgaataccg
    10261 tgggcgcgac gcccatgtcc gtcggttccc ttgagttcta tgaatccgac ggcatcgcca
    10321 ttatcccggt atttccgaac tggtccatgt atgcggcgac gcttgcaatg gcggggccga
    10381 agagtttgct gaactttccc cgattctgga agctctcggg catcatggtg aaagtgcggg
    10441 atgaaacggc gggcgagctt tttcagggtg tatccttttc caagaaggtc agcgaggcgg
    10501 accggatgaa gctcgataag ggagttgaca tcatcaaaaa ggtgtttcgc aaggccggcg
    10561 cgaaagattc gacgatcctt cctctcaatc cgcagggagc tcatccgtcg gcaacctgca
    10621 gaatcggcga tgtggtggac cgcaaccttg aaacggagat cgatcatctt tactgctgcg
    10681 acgcaagcgt cctgccttca tcgctggggt tgccggtgat gtggacggta gtggccctgg
    10741 ggaaacgcct tggaggccat ctcgcggccc ggatcggctg agcggcaatc cgcgcagggc
    10801 attcagcggg gcacgggcaa tgtccccgcc gttttgatgc agggtcgcga acgcaaagcg
    10861 cggctcaaga ccagccggca tcgctttcag agcgtgccgg ggcaaaacgg ttttttcaga
    10921 gaatcacttg aatggcccgg tgggctttga gttcctcgta cagaacgagc cggtgagatt
    10981 cgctctccac ggtcagctcc acgttgaggc agacgtaccg ggcggtttca ctttgacgcg
    11041 acagagacac ccggcaggga cggtcccgga taatttcgtg aaccgcgctt tgcatctcgt
    11101 ccggattccg gccgatgatt ttataggtcc agaggcacgg atagtccaat cgaagcttct
    11161 gtttttttgg atctgtcatg gtctgcctgc tttccgggag aaactatccg gaaaagcaaa
    11221 taaagtcaag aggttcatcc ctcttgttgc ccgacattcc ctttgacaag tccgccggtc
    11281 tctgctagaa gtttttgcac cggatccttc aacaaaaaac atcacacgag gaggtctcat
    11341 gctgaagaaa aaacgttttt tgtttttgaa aaatggtttt acgcttgccg ccttgatgct
    11401 gtgcgccgcc ctgattgcgc aaacggccgc attcgcgcag gccgccgaaa aatcagccgg
    11461 ggaaaaactg cctccggcgg tgcgggtcaa catcaacaag gccgaccagg cgacgctgga
    11521 gaaactgctc gggatcggcc ccgtcaaggc caaagcgatt atcgacggcc ggccctaccg
    11581 gaacattgaa gatgtcatga acatcaaggg catcaaggga aagacgtatg atgcgatcaa
    11641 ggaacacatc gtcgtcaaat aagcgtttcg tttcaaaaac tgcaaaggag gatggattga
    11701 tgaagaaaat gatggttttc gtttgtgcgt ttttgctgtt tgcggcggcg gcctatgccg
    11761 cgggccccag aacctatcag gtgaccgggc cggtgctgga tgtgaaaggg gatgtgattg
    11821 tcgtcaagaa aggaagcgat aactgggaag tggcgaaaaa tgccgccacc agggtcgcag
    11881 gcgacctaaa ggtcggctcc cgggtcacga ttgaatacac gatgaccgcg acgaagattg
    11941 acgtgaagga agacaagaag gcggacgcca aaaagaagaa atagcgattt caacagacac
    12001 acaaagccgc ttcggtagcc cgattaatga aagcggttgc cgcggtggcg caaaaaaaga
    12061 ggtcatgaat ggcctctttt ttttgccggt ttgacattcc cgcaatcgtc cttaaactct
    12121 tatttaagga aaacaggatt caaaagacgg gacggacaaa atgaaaaagg catggattgc
    12181 cgctggattg ctgtggtttg cgctggcctg tcagggcggc aacacacagc ccgtgaaggg
    12241 ggcggaaaaa atgaacggta tgacccaaac agaaaaagag gcagtcgcga cgctggccgg
    12301 cgggtgcttc tggtgtgtcg cttccgatat gaagaaactg ccgggcgtaa cccgtgtcgt
    12361 gtcgggctat gcgggcggtt cgggaaaaaa tccgacttac gggaactata tggcccaggg
    12421 gcacctggaa gccgtgcagg ttcattacga tccgggcgta gtgacctatg ggcagatcct
    12481 ccattacttt ctgaagcata ttgatcccac ggacgcggga ggtcagtttg ccgatcgcgg
    12541 ttccggatac cgcagcgccg tcttttacaa caacgaggcg gaaagaaaga cggccgccgg
    12601 ggctctgcaa tctctggccg catcgggcaa gttcggcaag ccgattgtaa cggagctgaa
    12661 gctttttacg ggattctatc cggcggagga gtatcatcag gactatgacg ccaagaatcc
    12721 gctgcgctac agggcttacc gcagcgggtc gggccgggaa ggatttcttc gggaagtttg
    12781 gaagaaggat cgggataatg ggccggcttc ggcgccgccc taccgaaaac cggacgacaa
    12841 aatcctgaaa aaaactcttt ccccgatgca gtacgacgtt acgcagaaaa acgcgacgga
    12901 gccgcccttt caaaacgaat atgtccgcaa caaaaaagag ggcatttatg ttgatgtcgt
    12961 ttccggggag ccgctcttca gttcgcgaga caagtttgat tccggcaccg gctggcccag
    13021 tttcactcgg ccgctggagt tgtccaatat cacgacgaaa gaggaccgca gcttctttgc
    13081 gacgcgcacg gaagttcgca gccggcacgc ggattcccat ctgggccatg tgtttccgga
    13141 cggcccgggg cctgcaggtc ttcgctactg catgaattca gccgccctgc gtttcattcc
    13201 cagagaagat ctggaaaaag agggctacgg ccgttatcag aaattatttt cacaggacaa
    13261 atagcaacgg tttcaaaccg ttgctctcca tccggcaata ttcatggcgt catgacatca
    13321 accggtgacg tcctgatcgt ggattgattc tccgcttgaa tgtgcgcggc gatttcgccg
    13381 acattacgca tggcgtcctg cagatcgatc gtcagcaggc gcctgttctc catgaccggc
    13441 tttccgccga tgatgctggc ttttacatcc gctcctcttg cggcatacac aatctgggaa
    13501 taggggttgt aaagcggcgt caggtgcggc tggttcagat ccagaatgat gatgtccgcc
    13561 tgttttcccg cctctatgga gccgatcagc cgatccagtc cgagaacacg ggcgccgtcg
    13621 atcgtcgcca tcttcaggac ggtttcggcg ctcatgaccg ttggatcgag cagagcggct
    13681 ttgtggagtt ttgccgaaag atccatttcc tgaaacatgt ccagatcatt gttgctggcg
    13741 gccccgtccg tgcccaggcc gaccgcgatg cctttttcca gcatcagcgg caccggcgcg
    13801 atgccggcgg ccagtttcat ggagctggac ggattgtggg atacgcggac gcccagatcc
    13861 gcaaaaagct cgatgtcctc gggtgtggtc cagttgcagt gcaccgcgac ggtccggtcg
    13921 tccagcacgc ccagatggtg cagatgctgc accggcttcc ggccgtagcg tttttcaatc
    13981 atgccgatct cgtcccggtt ttccagaagg tgggtcaggt aaaggattcc ggcttcgcgc
    14041 gcggccgcct ttacgttgac cagcgtccgg ggcgaacagg tgtaggggga atggcagaag
    14101 aacgcaggcg tgatcatcgg cgcgtgggga agccagcgct cgacaaaccg ttcggcggcc
    14161 gccatcattt tttcgtattg ggggtggtcc ggcgtggcaa aatcggcgaa tccctgagcc
    14221 gccaccgcgc gcatgccgca atccagcgcg gcttgggcga tttgattttc aaaaaaatac
    14281 ccgtcgcagc aggtggtggt tccggacagg atcatttccg cgatcgccag cagggcgccg
    14341 tcgtacgcca tctgccggtt cacgaagcgc gcttccatgg gaaagatgtg atggttgagc
    14401 catgccatga gcggaaggtc gtccgccagg ccccgaaagc agaccatcgg cagatgcgtg
    14461 tgcgtgttga tgagccccgg cagaatcagg ccgccgccgg cgtcgatgac cctgtcggcc
    14521 gaaagggaaa ggtgtgagcc cacccgggcg atgcggccgt ccgcgatgcc gatgacggcg
    14581 ttttcaacaa tctccatttt ggcggacatg gtcaccagag tgccgcccgt aatcaccagg
    14641 tcaaaatgag gcgatggcat gattttctct ctccgtcagt gaaattccag tttgtagtag
    14701 aggtcaacgc cgctttcctg ccccatctgg ctttccacct gccagttttt ggaaatgtcg
    14761 tagcgcagga aaaataaatt gccccccgaa aacagcgagc ggccgtagct gatgtacaac
    14821 tggggcgtca gatatttgcc tacgaccagg atggtttccg aagcgctttg cgccgaaccg
    14881 cccgtcccct cgggcgtcac tttcagacgc ctgtatccca tccggttgct tttctccagt
    14941 acatccgaag agatttccag cgaggccagc ccaagatagc cctgaatctg ttccagcaga
    15001 tcttcggcct gttgcgaggt caaaaccgcg gaggccgccg tggccagaag accggcctgc
    15061 tttgtgtcgg cgccgagcgg atgccccagc acgatgtagg ccagtatgtc catgtcctgc
    15121 atttgcggtt cggagtacag tttaatgacg ggcttgggaa gcgaaccgct gaccgcgacg
    15181 cccgccttca cgtctccgat ttttctaagc gccaggatgt ccagcgccgg ctgattgacc
    15241 ggacctcccg catagaaaag atggccgcgg acgatttcca gattgacgcc gtaggttcgg
    15301 aaccggcctt tgaccacgcg gatttcgccc cggcccgaaa tgcgctccgg atcgctgaag
    15361 gaaatgtcca tttcgccgcc cagcttcgcg tcgattccgg atgcgttgaa atgcgcctcg
    15421 tcgcccagcg ccattttgac ctgaacatcg aggccgaccg acggcttgcg gccgggggat
    15481 tttgtttttc cttcccggat cacatcggaa ctggatgccg cggggccctg cgattgcgat
    15541 ccgttgacct caagcagggg caaaaggact tctccccgga cggatatttt ttcaggcgtt
    15601 ccccaaaagg tcagccgggg agaagactgc acatgaagtt cgggaacgtg aacggtctga
    15661 aaccgctcgc cgtcgatgcg tccttcaaaa cccgcgatct gcatcccttt cagatgaatc
    15721 cgcgcggaac cgtccacata gccggggccg gaggccgccc ggaaggaatc aatgaccagg
    15781 ccgtctttcg acggacgggc ttcgatccgg acgtcctgaa gcgtgatgcc ggccgaaggc
    15841 agatagcccc cggcgtcgga caggcggatg cggccttcaa acagcgggtc cgccggggtg
    15901 ccgctcacgc tgatatccag atcgagcgtt ccgcggctct cctgaaccag cccggggaaa
    15961 agaacgccca gcgctccttt ttcctgaacc tgtccggcaa gcgaaccctg aagcggtccc
    16021 cgctcattca gggtccaccc aaagcgcgcg gcgaggggca ggcggatttt tcccttcagc
    16081 tctccgtggt ccgccatcgc cacggcgacg tcgcacgcca gagtgtcttt ccgccagacc
    16141 cattctgcgg atgcccggcg aaaatcgatg ctgacgtcgc ccctctgccc ccgccggtgg
    16201 atttttgagt ggtccagccc ggcccggccc gcgcaggaaa agcgcccgcc ggacagaagt
    16261 ttgccttcca tctcccccga cgttctgcct tccagtttcg ttcggcgggg gagcgatgcc
    16321 gaaaaccttt tcaggtccag ctcccgccag cgcacatccc attggcccgt gtcgggaagc
    16381 gactgttttg cgggctggga tgagaagaag gagccctgca gccatccgcc gtcagacggc
    16441 tcgaacttca cgtccagccg ggttccccgc ttgtttgcgt tcagggttat tttcccctgc
    16501 cggacttcaa cggtctgtcc ttgcgcgctc agggttccgg acagggttgc ctccaaggct
    16561 aggtcgatgc ggtttgaggg cagcagcgtt gcgtgaatga ttccgccgca cgcgccggtg
    16621 gcaatgccgt gatcggtcca ggcgttggcc ctggccaaat cgacgtcgtt ccaggtcaga
    16681 tcgacaggac cggtcagggg gtttccggaa agaaccgcgg acgcccgaaa cgcttccgcc
    16741 ccccggccgg tgatttccat cggccccagc gccagtcgtt cggcggatat ctccagggcg
    16801 gagggagagg acagcctcca ggggccgact ccgtccgtgc cttccagacg aattattttt
    16861 cccttccaga ttttttcccg ccgggcgccc gacaggtcca ggcgcgtttc gtgggggccg
    16921 cggcgcatca cggcggccag cgtatgggaa agggcggttc ctccgccccg cactctcagc
    16981 gtatccgtcg tcaggctccg gaagcgaagc cggctgaaat cagcggtcag cgcaaagggg
    17041 gacttttccc ggtcttccag ggaggcgctg aaattcccgg actcaatgga ccagccgtcc
    17101 gccgcgagag agcggccccg ggcggcaacg acgccgctga accggtcatg tcgccggcgc
    17161 gcccaccctg aagctgtaag cgatcctgat gtgtggggaa tcagcctcgc cagatcgctg
    17221 atttcagcgc tgaaatccaa ttggtccgcg acgcttcccc tggcagcgaa ctgaaagccc
    17281 tttcccctga gcgccaggcg ctgaatgtga atatcgttat ccgcaaaggt tgccctcaaa
    17341 tctcccgtca actgccggcc gtgcaggcgg ctttctttca gcgtgagatt gaaagagccg
    17401 ctgggcggcg cttccgccgg agcggacagg tcgccctcga gatcgaaatg gacaatgccc
    17461 gcccagtcgg gattccattt tgccggattg agattttttc ccgaaagcgc gccgcttatt
    17521 ttcaggccgt ccgtccatcc gatcttcagc cgtccgttca gggagccccc cagcgccgtt
    17581 gcctgaatcg ggctcagggt tgcgccttca gcgttccctg aataatcgcc cgccagacga
    17641 aaagcatgcc gggtgtccgc cgtattggac agaacgacgc gcccctgata ttgccggccg
    17701 gttccgtcaa ggaggagaga gccgttgaat ttagcaggaa ctttcagctc gtcggaaaga
    17761 tccatgtttt ccgcctgaac cttcagggct gcatgcggcg tcgggccttt gaaggccaga
    17821 aagccctcgc cggcaatgga gcccggacga ttcccgcgcc gcagaaggat tttggcaaag
    17881 gtcagcccgg cgggcgtcat gccgatgtcc gatgatatcc gcaaaaccgg ccgggtattt
    17941 tgaaggccgt gaatctgaag gcttcccttc atttctcccg gcttctttcc cggtccgaag
    18001 gctgcgtcca gccgaatgga atcaacgccg gcggccgggt gcgccggagt tgcggcaagt
    18061 ttggctttca gcaggggacg atggaaaccg gccagaacgc ttccctccaa aacgccgaaa
    18121 gacgaggtga ggcgaagatc gcttacggac agactggaat tcttccagac ggcggccgcg
    18181 gcaagagccg tgatttgaac cggttcttca ttcgggcggc gataggtcag attcctgacg
    18241 gccaggcgcc cgatgctgcc gctcacgaac gtgatccagc ctttggcccg cggccagacc
    18301 agctccggct gtttgtccgg agagttgtcg aaaacggata cctggtcggc ggaaagcaga
    18361 gaaaacctca gatggcccgc catcaggtct tgcggccgca tggaaagccg cagctttcct
    18421 attcgaaggc ttccctgcgg ccactcgatg accgtgccgg tcaggcggag ggaatccgcc
    18481 agatttccct cgacggtttc cgccctgatg ttcacttccg tgaagctcga aacggtttcc
    18541 agcagttttt ttccacccgc ctcggtgcca aaaagccagc ccgcggccgc gagaaggacc
    18601 gccggcacgc ccagaacgac gacgaggatg agatatttca gcacgcgctt caaaattcaa
    18661 accccacggt aaagtgaaag cggaattccg ggtcatccac gcggatctgc cgggcgacgt
    18721 ataaattcag ggcgccggcc ggcgtgtggt agtgaatgcc gaagcctccg gcctgataca
    18781 gcgtcagatc ggaaaaagtg tcaaaagcgt ttcccgcgtt gtaaaacacg gagactgccc
    18841 agtcctccag aatgtcgcgc tgcagttcca cgcagccggt cagcagatgc ttgccgccct
    18901 tgacatttcc ctcggcgtcg cggggaccca gcgactggta ggcgtagccg cgaacgcttt
    18961 gatcgccgcc cgcaaaaaac cgcaaagacg gggggaggtc ggaaaactcg tcggtaaaaa
    19021 ccgtgaagcc cgctttggcg ctggtcagga aagacaggcg ccacggcagg ggaagaatgt
    19081 ggctcgcctc gccgatgaac tgaagcaggc gggttgtgga gcccagggcc tggtgcgtgc
    19141 cgcgcagatc gagcgcatac cggaacccgc ggtcgggccg aatcaggttg tcgtagtggt
    19201 tgtcggagaa ccggacgccc ggaaggacgt atctcgaggt tgcctcctgg ctgccgatgg
    19261 tgaattcttc gtgctgaaat ttgatgtacg ccgaaccctg ccttctggga gtgaagctgt
    19321 gcgtccgggc cagttccatc gcgatgatcc ggctgtcgta ggtggtgatt tcctcctgct
    19381 gaaggttgat ttgcgcggag gtgaaagtcc tgatgtccct gccgtccgga acgatgtagc
    19441 tcgtcgcgag tccctgcagc cgctgggaga tgtacaggtg cgaatccagt tcgtgccccc
    19501 ggtgaagcat gttgaggtcc cggcagcgca aagcgaatct cgctcccgta tccgttccgt
    19561 atccgatgcc cggcctcagc gtgatgcgcg gcacgggcgt caggagcacc cgcaccggca
    19621 cttttccttc ccgggactgc tccttgtcgg cctggaccga cactcggcga aaccgctccg
    19681 agttgttcaa ctgaagctgg gactcggcca ttcgggtgta cgagaaagcc tctccgggct
    19741 gaaaggcgag gtagcgccgc agaaaggcgt cgggataatc gggggcgccc tcgatggttg
    19801 tcgtggagaa aaaatatttc tcgccggttt ccagcaccag cgtgatgacg gccgacaggc
    19861 cggcctcatc tacccggatt tcgtgaacgg tgtaggcggc gtccagatac cccagctctt
    19921 ccgcccggga tttcagcgcc tgcttggctt tttcgtaaag aggatgtgac agcgcgtcgc
    19981 cttttcgcac agggaagtcg gcggcaagcg ccgccagcgt tgtctccgac gcgccgcttc
    20041 cggcgacggt gacgctgacc ccggaaacga cggccggctg acccggttcg atttgcaccc
    20101 gcagccggta ttcgtccgct tcgggggttt cgatcaggac gacggccctg gcggaataat
    20161 atccgaaggg ttcaagggcc gccggcactt tccgtccggc ctggcggaca aaccgctcca
    20221 gccagagccg gtccgctttt ccatccttta ccaggccggg aggcagggtg agcgcttcca
    20281 gaacattttt ccgggcctct ccgtcaacgc cttccacaac gatttccacc gggccggcgg
    20341 ccaggacgac gacggccggc cccgtcgcgg acaaaaggcc caaaaggaga aaaacgcagc
    20401 accaggttcg gaaggccgaa ggggacgccg tactcgccgg atgggaaagg gtcatgatct
    20461 cgatggattc ctgatggttt tctattccac tttcagcgtc gccgtttttt tgccctgaag
    20521 gttgcagacg gccgtcactt ccagaagatc gtttggcttc gccggcactt tgtaggtata
    20581 gacgaaggtg gctttgccgg cctgtgaatc ataatcggcg gtttcaagcg gctcctgatt
    20641 ttttttgatc tgaacctgct tgatgtagtg gaagccggtg aacgttgatt tgtgcgtgat
    20701 ggatgccgaa agcgtctggg tcttcaggtc gtaactcagc gaaaggtcct ggggaggatc
    20761 ggcgaaggcg ggctcagccg ggagccaggc ggcggccatc cagaggaaaa tcagcaaaaa
    20821 gatcaaaaga tgttttttca tgtcgcattc tcctgaaagg attttgttgg tcctacagta
    20881 atacaattgg tccggaaatg acaacaggaa aatagggatt gaaaaaggag ggacaattcg
    20941 atattcttcc ttccactaca atcccggagg aagctatgga cgcgttgctc cttgcccgcc
    21001 tgcaattcgc ctttaccatc ggattccatt ttctctttcc ggccatgacg ctgggcgtcg
    21061 ctctgatcat tttgatttcc gaaacgctcc atctggctac gaaagatgac gtttaccgga
    21121 gaataacgga tctgctcgcc agattgctgg ggctgatctt tgtgatcggc gcggccaccg
    21181 gcattgtgat ggaattttct ttcggaacca actggtcccg gtattcacga acggttggcg
    21241 acattttcgg gccgatcctt gccgccgaag gcgtcatcgc tttctttctg gaatccgttt
    21301 ttgtaggggt gttgattttc ggcagaaaga aagcatcgcc cggcgtttac tggctttcgg
    21361 ccctgctggt gttcatcggc ggccatttgt cggccttctg gattctggcg gccaactcct
    21421 ggatgcagac gcccgccgga tatgaaatca cctccgccgg taaaatcgtt ttgacggact
    21481 ttggcgccgc cgttttcaac ccgtccaccg ttgcccgctt tttgcacacc gttttggcca
    21541 cctgggtcac cggcgccgtc ctggtttgcg gaatcgccgg ctactacgtc cggaaaggcc
    21601 tgcatgggga gacggcccgg atgatgctca aaatcggcat tattcttttc gcggtcacgc
    21661 cgcttctgca gttgggcgcg gcccacggcc atgcgatcca ggtcatcgac tttcagccga
    21721 tgaaggccgc ggcgatggag ggacatttta ccaccgcccg cggcgcggat gtttacgttg
    21781 ccgggtacgt ggacgagaaa aatgaaaaaa cttacggaat ttccatcccc aaaggtttga
    21841 gctttttgta caacttcgat ttcaactcgg aaatcaaggg tctgcgggaa gtgcccaaag
    21901 aagactggcc gccggtgaat ctggtgtttc aggcctatca catcatggtg acgctgggca
    21961 tgatttttat cggccttggg cttctggggg ccttgctttt gtggacggga aaactctaca
    22021 ccgcgaaatg gtatctctgg cttttgcctt tcctgattcc cctgccgcac ctggcccacg
    22081 aaacggggtg gatcacggcg gaagtgggcc gccagccctg gatcatccag ggattgatgc
    22141 gaaccgcccg ggcctcgtcc gtggtggtat caaaaggaga actggctgcg agcctgacca
    22201 tgtttgccct ggtgtattta ctgttgctgg tcatgtttgt catgctcttt ataagaatcg
    22261 tcaaacaggg gccggaggct tcttaaatgt gctgtaaggg ggatcgactg cgatggaaaa
    22321 tattcaaaac tttcaaatcc tttggtttgt cctgattttt gtcctgctgc tcggatacac
    22381 gcttttggac ggattcgatc tcgggctggc cgcactgctg ccgtttctgg gcaaaacaaa
    22441 agaagaaaag gacacgctca ttgcttccat cggcccggtc tgggacggca acgaagtctg
    22501 gctgattacc ggcggcggcg cgctttttgc cgcctttccc cacgcgtatg ccaccgcgtt
    22561 ttccggtttt tattcagcga tgatgctggt gctgctggcc ttgatttttc gcgcggtttc
    22621 gatggaattc cgggccaatg atccggcgcg cgccggtctc tgggaaaaaa cattgattgg
    22681 cggaagcgcg ctggcggctt tgctgttcgg tgtggcgctg ggcaatgtga tctacggcgt
    22741 gccgctggac ggcagaatgg aatatgctgg aagctttttc acgctgcttc gtcccgtccc
    22801 gctgctgttc ggcatcaccg gtcttgccgc cgtgctgctg cagggcgcgt cctgggcggt
    22861 gctgaaaacg gaagggcggg tgcaggagcg ttcatttgaa gccgcgcgga ctttgacttg
    22921 gatcaatgcc gtcctggcgg tgatttattt cgtggttctg atttacgcca tcccgcgcgt
    22981 gtcgggaaat gcgttgtttt atgtcgcggt tgcaatcacc tttgcgggac tgatcggatt
    23041 gtttttttct cttcgcaaaa aaaatgacgc aggcccgttc tgggaatcgt ctttttcgct
    23101 tatcggactg tttctggcgg cgggcgcggc gcagtttccc aacctgatta ccgcttccaa
    23161 cgatccgaac ttcagcctga ccatttacaa cagctccacc agcctttaca cgctgcaggt
    23221 catggccgtc atcgcgcttg ccggcatgcc gattgtgatc ggctacaccc tctttgttta
    23281 tcgcctgttc aagggaaagg ccaaagccga cgggcattat tagaaagcat ttttcaggcg
    23341 tcctgcctaa aggatttgat tggccggggt ctttctgagc agcgcggtca aaagccagct
    23401 tcccgccagg gccgtcagga aaacggccgc aaacttgatc cagggggaaa gcggctgtcc
    23461 cagcaggaaa aactgcatcc agatcacgaa gacataatga aaaacataga tgccgtaggc
    23521 gttggcggtt agatggtccg ccacggggcg ggcggtatgg aaaaatttgc gggcaagccc
    23581 gagggcggca agccccgtga aggtgcagca cagacagtag agggcggtca ggatgacgct
    23641 catcagccat gcaggggtga tgccgaggcc ggcggcgttc acttccagcg ttccatgcgt
    23701 caggtaggtg atcccgccca gaaacagcca cagcggccac ggcctgagat tttcccgcga
    23761 aagggagcgg tccggatctg cgccgcccag ggcgacgccc agcagaaacc acacaaagta
    23821 caagaagaat cgccaggcgt gaaaatacag ggggcctgcg atatgcgccc agtgtccggg
    23881 ttcgacaaac aggcgcagcg gaagcgtcgc ggccaggctg gccaggagaa agaccacgac
    23941 cagatttcgc ggcgatgcgg ccgtccagga taactttttc atgagcccgg gggtgaaccg
    24001 gcaagccagt gcgacgaagg cgccataggc cagcaggacc cagataaacc aggccggccc
    24061 gcttgcccag ccgtcgttcg taaagaagcc ccgcagatag ccgccctggc tcgcggtgtt
    24121 gcttaaaagc catgacggaa agtaggccag cggggccagc agaaacacgg aaatgataaa
    24181 cggaacgccg agacgcttgg cgcggtcggt cataaagcgg ccggcgcctt tccggttcag
    24241 ggacgggaca ataaagagtc cggagatcag gaacatgagg cacatgaaga acatgtcgtt
    24301 ccagcccacg aaaaagtcca gcggcagcaa gcgaagcgca tcgacgacgg gggccgaaga
    24361 tttcatgtaa tcgtctgcat tatagaaact ggaggtgttg taggccagag aggcatggtg
    24421 ggcgaccacc agcagggtga cgccgctgcg cagatagtcg atggcgatat ctcgtttcaa
    24481 aaagttcctc cggacggcca agggatcggt ttgttcggcg aggatcgtca aaagccggat
    24541 cttcgggcgg cggccgtcgc atcgtcagta ttgcggctga tccgttgggc gcttttgacg
    24601 gaagcgcact ataggcaatt tatttcggcc tgtcaacccg atccggagca tccgagaaaa
    24661 atgctccgtt catcattgct tactgcttga acagtgaatg gtcgaataga attggaaaag
    24721 caacagcaca ggaagcaagc aaggcccgga taaatcttaa aaatattctt atcgctttcg
    24781 gaaatctctt ccaatgttat tttttgccgg gcagtaaatc ggcgtttgcg tcgatctcgc
    24841 ggtccaccgc caggagcgcg ggcggcagcg cagccgcggc gcgcaggcgc cggatcaggc
    24901 cgcaggacac gcaaaagagc tgagccatga cttcgtccac gatttcgcgg ggaatgccgt
    24961 agtaaagaaa gttcagcggc attccttcgc ccagaaggta aaggatcttg ccgcaggacg
    25021 gctgatccgt gaaggcaagc cggatttcgt aaccctgcag aatgccggtc tgcaaatccc
    25081 gcaagggttt gaaatcggcg ctgaccggcc ggccgccgat gcgcgcattg ggggtatcgc
    25141 gaagggattg cagataatgc tgtgcgtcgg caaactctac cgttttggtc gaaccgctga
    25201 tgaaaaatac ggccggttgc ctgccgttta ggagaatttc ctccagatgg ccgcgctgca
    25261 gaagcgatag gccggtaacg ccgatgaaca tgtcggcgtt tctcagtacg ccgcttccca
    25321 gctcatcgag ggttttggct tcctggatgc cgtctgcggc gtcgtttccc gcggcgacgt
    25381 ccacgccgaa gagacagcct ttggtcaggc ggcggccgag ttccttcctg agaaagccgc
    25441 cgatggcgcc ggccgagccg aggatgacga tcgttcggcg ggaaaggagc aggcccaggc
    25501 ggtggagaat gttttccgcg gcgttgagga tcgagacggc gcaggccctg gcttccggac
    25561 ctctttttaa atccgaaaca gcgatgctga gcgccggata ttgcagtttt ccgaacgcct
    25621 gcgcgacctc gtttaaataa tcgtatccgt ttttcgtatg ttcgacgctg cccggaaaca
    25681 ccggggccag ccagtccgcc agcggcatat ggaaagcctc cgcggggcgg ggcaggtgga
    25741 agtgttcaaa aacctctccc agcgttttcc cctccagaca aaagcggttg atgatcggcg
    25801 cgaggtagcc gccgtcctcc accagcagca gcagccggtt tgcccgccgg gccgcatcgg
    25861 cctctttgaa aaaaaggtgt cccgccgcca tgcgcatggc gtccaggaaa tccggctgtt
    25921 cgtcgatcag cgcctgttcc atcgtttcca gcccggaaaa aggagtgaac tgccgtgaca
    25981 gcatgtagct gccggccagt ctgaggcgcg attccatttt ctgaaggccg taaaatcgaa
    26041 atgtctgagg cggcaggctc atgagggttt ccagataatc gttcggcaca atgccggcgt
    26101 actggacgaa aaaagcggtc agcgaatcgc attgcgcgtc ggcaaaggcc ttcagcagac
    26161 cgagcgcttc cgcggttgca tgatgaatga cgaaaagcct tacgccctcg agcgcccggg
    26221 gatggagcgc caaagccgct cgaagcgcct tcatcgacgc gaggtaatcc gccagatcct
    26281 gcctttcgcg gatttcgtcg aagctcacgt tgaggcaggc gccccagcgg gaaacgtcga
    26341 aatatatgcg aaacgatccg gaaagcatct cgacggtctg ccccttttcc agcagatgca
    26401 aaacggccgg atcctccagc agttgaatct ccctggccag catgtcgagc gattcggtca
    26461 gggcggtcgc gtcgcttttt tccagaaaca tcatctgccg gtcgtctgtg ggccaggtaa
    26521 agcgaatctg ctgcagttgg ggcaggccgg tttgccgcag ataggcgaaa aagtccggca
    26581 ggtgcgccga cgtttcaaca tgctcggcgc gatgattgag gacgtcgtgc agaatgccct
    26641 ggtggatttt ttcagccgtt gaaaagatgt cggtgaaaaa agagcgccga agcggctgat
    26701 cgaccacgcg atgaggaatg tgtttgagct gaaggcgcga atgggccgcg tcctcaatcg
    26761 tgatgcgcag aaaatggccc gatgggctgt gctccaccag atagtaatgg ccgtttggat
    26821 ccccgccgtc gtgaagatga aacggaaaca gcagagcggg cggcgccgac gcctcggacg
    26881 gttgcagacc gaaatagccg ggaaagagcg cgccgcccca ccgttcggtt tggagcgtca
    26941 gaaaaatcat ctgcagtcga tcgccgaaac tccggtaatg gaagggcaat ccttccgccg
    27001 catcgagcct tacataaatt gttacccaat ccagaccggc ggcagccagc tccggcgtca
    27061 ggcgggaatc cactttgcgg cgcaccatga tgtcggcggc gccgaaagcg cactgctcga
    27121 tgatctcctc cgccaggagc gagacaaatg tgtgcagatt gcggaaaacg cggatgcggg
    27181 aaagatcgaa ggcgatgtga cgctcgcctc gccggatcgg cgcaacgccg agcgtcggaa
    27241 gttccccggg cgcgccggct gttgtttcca gagcttcccg gtcggcctga ttccgaatca
    27301 gggttgtgag catgtcgcca acgtcgcgct tcatcttttt ttcgccttcg tttaagggtg
    27361 aatgtccagg agaaccttca gcgcgtcgct ctgtgtcgcc cgggcaaagg cctcctccac
    27421 gcgcgccagc ggatagcggg cggtaatcag ccggtccagc ggcatgtccg gatattcgca
    27481 catgattttg agtccgtcgg caaaccgtcc gcagcgcgat cccagaaccg tctgctcatt
    27541 gatgacgatc ggcgccagat tgacgtcgcc ggaaacggca acggtggatt tcagcacaat
    27601 agttccgcgc ggccggcaca atgccatggc tgtgacgatt ccggcgcccg accccgtggc
    27661 ctccacgacg atatccgcgc cgggcgcaac atccttttca tggaggacgg tccgaatgtt
    27721 tcgccaccga agattctcca gtttttccgg gtgatgcccc accaccgtga ccaccggtaa
    27781 aacggtggcg agcacccagg cgcaaagagc gccgaggcga ccgtcgccga gaatcaccgc
    27841 ccgttcgttg cccgcgggcc gcatttgctc caggatttcg caggccgccg acaggggctc
    27901 cacgagaacc gctcgttcgt cggaaagttt ttccggaagc gccagcaggt tgacgatggg
    27961 cagggtgcaa tattcggcca tgcacccgtc gaggttgaga atgccgaggg tcgaacggtt
    28021 cgggcagtga cgccccagat cgcgccggca ccagtcgcaa tgcccgcagg cggcgttgat
    28081 ttcgccgacc actctcttgc cgatccagga ggggtcgtcg cattccatga cgatgccgac
    28141 aaactcgtgc cccagcacgc ctttgaaccc catatagcct ttcaaaattt ccatgtcggt
    28201 tttgcagatg ccggcgatct ttacttgtat tttagcccag ccgggcttga cgggcggcac
    28261 ggaataatcc tcgacatagt tgagtttgcc gtcgaaaacc gccgctgcca tagtgtccat
    28321 cgtccccatc gtcttctcct tatgtttccc tgaatttgcg ggctttttct ttcagcgcaa
    28381 ggaaatattc cgtttcaaca atttcggaaa catcctgcgc ctgctttttc cggtcgatga
    28441 taattttcag ttcatcgacc tgtcgttgga gcgttttttc cctttcccgg acttttccgg
    28501 ccatctgctc gaaaatgcgg gccagccggc ccagttcgtc tgcgcgcgcg gacacgcccg
    28561 ccagtccggg cagatcgtag ttgccctgtt ccacggcttg cgcggcttgg gcgagatccg
    28621 ccagcggatc ggtcaccttt cggacaaaaa cgatcgcggc gataaagccc gcgactaaaa
    28681 gcaggacgga aacgacggcg gcgatgtaaa agttttccac catcgcgctg tgggaaacca
    28741 gatcgtcgag gaccgcccag gtgatggcgc cggcggtggc cagaatgctc acgagcagaa
    28801 ggccggtgat catcaggatg gtccgcgtct ccagcgtcag gcgaatgcgg cgggccagaa
    28861 ggagaatgac cggcaccaga atgaaagcgt tgaccgcgtc ggtgagaaac gcaggcagaa
    28921 tccaggacgg aaaggaatag ggaaaggtga gcagatgaat aaagaaaacg tcgagcacga
    28981 ccgccgccgc aacgcccgcg gcgctggccc cgaccaggct catctgcagg atgccgaatt
    29041 cccgcaccgt gccgatgcgg cggattcccc cgtagccgac aagccccgga atccatccca
    29101 taaggccgtt ggcgatatgc cagttccaga atttaagagc gccgaatccg taaacggcgt
    29161 cgccgaacat gttgcccgca aatccagtga tgaagccggc cagtggatgg accagaatgc
    29221 ccacgaccat cggaagagcg atctgcggcc gcatggagat gatcggcgcg gagggcagca
    29281 ggaaaatgtt ggaaaggctg ttcaagagcc catagacgac ggcaaaggcc aaaccggcca
    29341 ggaggatttt ttttgcgccc ggattcatgc cgtaattctc cgaaggatat cttccatcgc
    29401 ccggccaagc gccgtattcg gatgcatcag acaaaacagg ccggagctgc ccagcgccat
    29461 catttcgtcg gcgtgcggca ggacgccggc cacgtcgcag ttgtatgccg ccgtcacttt
    29521 ggcggccagc gcgtcgcccg ggaaagccgt ggggactttg ttgacgatca gaaacattcg
    29581 gggaacttca agctgtctcg acacctcgac ggtcacggct gttccctgat agtcctgctg
    29641 atcggggcgc agcagaatga ggagggtgtc ggcgacggcg atcgacatca gcgtctcctc
    29701 gttcaagccc ggatgggtgt cgatgaaaag ataatcgagc gcaaattgtt caatcgcttt
    29761 ctgatagcct tccttgagca gattgatgtc gtagccgtaa tgcaggaccc gggcgatgtc
    29821 acccgtgcgg atgctcgacg gaatgagaaa aagcttttct ttatcctccg ctccgagaat
    29881 ccgggacacg tcgtaaaccg cgcgtccgat gtcgcatttg ccccagagaa aatcgtttaa
    29941 ggtatggggc gcgtctttgg gatcgaggtt gaacagcacg tgaatgccgg gagactggat
    30001 gtccgtatcc acgacgccga cgcgccggcc ttttcgggcc agaaggcagg ccaggttggc
    30061 cgtggtgttg gtttttcccg ttccgcctcg aaaggaatga atcgcgataa tttcaggcat
    30121 ggcgtcctct ttgccgaagt cggccgcagt atagaaaaag cattgtgcca agtcaaggat
    30181 atcatgtttc aaaaagttcc tccggacggc caagggatcg gtttgctcgg cgaggatcgt
    30241 cagaagccgg atcttcgggc ggcggccgtc gcatcgtcag tattgcggct gatccgttgg
    30301 gctcttttga cggaggcgca cgataggcaa tttatttcgg cctgtcaacc cgatccggga
    30361 gcatcggtca ctgtatttcc tctacgctcc aggaaacata gccggacatc gggaaggatt
    30421 gagcgtgctg cgcttcgatt cggacaatgc cgtccagacg gcgtggaatg cccaggcgca
    30481 atacgccggc gggggttttc cagtcgctga tgcggatgaa ttttccgtcg cgcagatcct
    30541 gggccagact ggggctgaac gcacccactt cctgtgtggt cgcggcgccg aactcgcggt
    30601 tcagccgcgt tcgtatccgt tcatacaact ggtaggcgtt gtccagttct cccacctggt
    30661 tcagatcaat gatgacggcg gtccgctcca gcttcttcat ctgctcggtc agcaccagcc
    30721 tctggcgctg ggaaacatcg tcaaaccaga cggtttcaaa aacgagattc cgcccttcgc
    30781 gggttccccc cttgatgttt cttttttcca ggcccgccaa agcccggtcc atgggcatgc
    30841 cggggctgag atcagccagc gaccggatgg gcggctggac gtccggcgca atttccggcg
    30901 gagcaggggc ggccttcgcc gcttccgcgg gcggggcctc tgcgggcgcc gcctttacgg
    30961 gcgccgccgg ccggtcaaag ctgaaggtcc aggtcagggc aatccgggta tccgcgctct
    31021 cgtccgagtt ctgaggtttg cggtagccgt aatccgcttc caggcgaagt tgatgccgtt
    31081 tgtaggcata ttcacaggcg gcgttgaggc cgtgggtcag attgtccacc agtccggtct
    31141 ggcgatcctg attcagatgc cggtagctca ggctgaaact cagggggccc cggctcagta
    31201 aggaggaaaa attcaatccc agatcatcgg agttctggcc ggtctggcgc aaaatgctgt
    31261 aaacgattcc ggcgttggcc cggcctgaaa aaccggccag cgccagacca tgccccaaat
    31321 caaatccgta ttgctgggtg cggttgtcgg cggatatgcc gtagaccaca tgggtgtcct
    31381 gaatattggc cgaaagacgg gatgtccagc cggccggcag ctgactggtc agattggcgg
    31441 aaagcgcatg gatgatggtc ttggccgggt cggattcggc ctgctgacgg taggcgtcca
    31501 ggccggcatt gacgcccctg acccagggat tgggcacggc gccgctcacg cccaggccgc
    31561 agacgtacgt atccgtggtg accgccgaac cgatgccgtc gcgatagtat tgaccccggc
    31621 cggtaatcga atagttcaag gcggattgcc acccggcgcg ggcgtctatg gtgcgccgcc
    31681 cggcgctgac gacgcccccg cggggaatat aggctttgtc gtattcttca tagcgcaggc
    31741 tgtaggtcag gggaattttg ccgcgtccct gaaactgggc aaacgcgccc agcccttcat
    31801 ccttgaagga ttcgccgttt tgggcgttgt ccccccgaag ataattcagc tcggcatcca
    31861 gcgacaactg ctgggccaga aaattgaaat tgtgattgac ggccagcccg gtcacatact
    31921 ggcgcaaatc gggagtgccg gattgcggcg cctgatcgtt gtaggcaaaa ttcaacgacg
    31981 ctcttgtccg gctccagttc atcatccagg aggccccgcc aaaaagattg tccgccagac
    32041 ggttgtcgaa aaaatcccgg gacgcgccgc cgccctggag cagcagggag ttttgcgctc
    32101 ccaaaaactg gccgggctgc agctcaagct gggcgccctt gagcgaacgc tgaagaacgc
    32161 ggggggtttg aaagccgtga aagtcgccca ggtccatccg gtagggaacg ggcgcttctc
    32221 cattttcctg gttgatgttg aagcgctcca ggatcacgcc gtactggggg gagcggtagg
    32281 gcgatccgct gatggcgccg gacgcgtagc ctttgatggt attgtagggg ttttgaatgt
    32341 tttgaaaccg gatattgaga tcgttgtaag gcctccagcc ttcctgaagg taggcgccgt
    32401 ccggcccgtg ggaattgtag tagtccaggt gcatgctgtt ctggccggaa acggaccagc
    32461 cgggaaaaat ggcctggacg tcaacggcaa aagccgcccc gggcgggctg cacagcatca
    32521 ggcccagcag aaaacagatg atttttgatt tccggaagat ggatccgcta atcaattttt
    32581 tccacgacct ccatcaccat ttcattggtg cccggcgcgg aaatgaatcc ctgcttcgga
    32641 tcgttgaact ccgcgcgtgt ttccagaatg accgacacgt tgctgacccg cagatgcccg
    32701 ccttgcggat cgggaacaat cgtttgattc aacatggagg cgctgtggct gttgagaact
    32761 ttcagccggg cgtctttggc ggtttcggtg ttcatggcgt ccgtcaggcg ctgctgatcg
    32821 tagaagttcc cggaaacgag ttttttgaag tccggactgt tccaggatgc ggcaatctcc
    32881 tgagcggcgc gggccagagt tcccgaaggc agcggctgcg acggttccac cggctttgcg
    32941 ccgggcggca gttttgccgt tgcatgggcc ttgctgcgga tgggagacca ttgcctcatt
    33001 tcctgggcct gtccgggcag ggcgatcagc aaaaggatga agaccattaa gtattttttc
    33061 atggcagggc cctcacggat gcgtcgtaaa cggtaatgga attatagcgt ccgaaatgac
    33121 tgtagggaac gggctgcaat ccgccgttcc ccgccgaaaa tgtcacctga tagcgggttg
    33181 cgccgatctg gatgatgaac tggttgttga tggaggatgc ttccaccgcc cgcagaccgc
    33241 tgccgcacac gcctgtgtac gtggaaaatc cggcgccggg cgtaatcggt tcgccgctga
    33301 tcacgtcaaa gaacaccttg ctgccggcat ccgaacagga ggtatgggga ttctgaatgc
    33361 cgatgatcgg cgctccggcg ggattctggt caatggcgca gcttgccgga agcgtctcca
    33421 gccgcaggaa aatccaggtt ccgacatttc cgttcagagg ataaatcgcc ggcatgcagg
    33481 ccaccggcgg cccgggcgcg ggaccggggc cgggcccagg cccgggagac ggtgtttccg
    33541 atacgatatg gagcgttccg ttgctcatct gctgcaattg cttcaccgcc gacagagttt
    33601 cggggtcatt gaggtttagc gaacctgcgc cttttgtctg aatggtgacg gctgttttta
    33661 aaagcagcgc cggatcggcc acctgtgttc tgtcgaaagt atacgtgact ccgttgagag
    33721 taaaattgta aagcggctgg gccgtattca cgaagttgtg gccggagatc acccggtttt
    33781 ccagaatttt gactgtccac gttccggggc tgagcttgcc ttcggccgtc atctgcgccg
    33841 cgggccgctg ccggtcctga agctgccgtt tcatctcggg ggaaaagacg ggctgcgtgt
    33901 cccgaccaag aacattttcc gagccgagtc ccgccgccgg cgcttcgaaa acggcaaagc
    33961 acggcaaaag caaaagcatc agcagcgccg acgtaaaaaa agcactgcgt cgattcatca
    34021 gttgttgacc cattcttcca cctgtttggc gtccacatcg gcgcgcatga tctgtgtgcc
    34081 gcccggtata aactgaccct ggcccgccgc cggatgagcg taaatcaatt ccaccgtagc
    34141 cgtggcttcc gtgtcgaaat ttccctgaaa tccctttacg tcattaaaac atgcgccgtc
    34201 gccgcccacg cgggtgatcg gactttgcgc ctgctcgaat ttgagcgatc cgccctgatc
    34261 cagccgccag cggcagtgca tccggtccgg atcgaaatgc ccggtaacgg cggcgcccaa
    34321 ctgcgtggaa tcgccgaaca ccatgagctg acggccttca tttttcaggc ttttgagaat
    34381 ttttttctgt tctttctggg ttgcccgctg ctctttgatt tctttctttt ccgcctcgcg
    34441 gatcgtatcg caaacctcat ccagggcagc atccgaaagg ttcccgtatt cgccgggata
    34501 ctgagtgcgc agctgcctgc ggcagtccgt ttgcgtggtc aggcttcccg cgtccacaag
    34561 cgggatggtg tcgatcaccc ggcccggcac ggtgaccggc atcagccgga tgcctgcctg
    34621 agcaacaacc acgttgtcgg agaaaggcag catggccact gttttgccgc ctttgagaat
    34681 ttccactttt ttaacggcgg caacggcgga caatccggcc gaatagcctc catcctcgcc
    34741 ctgaataaaa ggcgttccgg tgctgccgcc ttcaaaatgg aagagcgcct gatattgatc
    34801 catggccgcc ggaccgatga ctctcagaac ataaggggtg aagccgccgg aggcttttcc
    34861 ccgcgaaagg ataagctgat tggccgtcac tttaacttga ttggaagcca gcatggcccc
    34921 tttggcggtt tcaaactgtc cgcgcgggtc cagatcgatg ccgcccaact cggcgctgat
    34981 ccaggcttct cccgccgttc gtcccgtctg gccgccgttt ccgtccgcca tggccaccgc
    35041 cggagtgtcg ctcttcaaac gcaaagaagc cccggtaaga aaacgtttga tggtttccgg
    35101 cgccagctcg ttgcctttat cgtcccgata gatcggcaga gccgcaaacc ggttgccgtc
    35161 gggatgataa ttgggaaaga acaaatcgac gccgtcgcgg gaagcgggtc tgcacttggc
    35221 ggcgagcgtc aactccaggg aggcgacgct ggggacgaca ttcaaccgcc gggaccagga
    35281 aagccggaca tcttcatcgc cagcgtcacc gaccgtcacc tccacatcca gttgaagaac
    35341 cgttcccatc ctggttcccc cgggaacggc gacaacattt tgcgcctgcc aggcattgcc
    35401 cattttttca aatttagctt gcggattttt ccactggccg cctttccatt tcactttgta
    35461 cttggccata tcggccgggc cctgcacctg cagcgccacc gaatagcttc cccctggcga
    35521 aaccgcgccg tcggccagag cattatccaa aacagcctgc cagcgattgg cggtgagctt
    35581 gcggaagaga gaccaatgct gacccagttg aatgttcaag cgcgcctcgc cgatgctgcc
    35641 cagcggctgc acatcaccgt cctggctgac cgcaaacggt ccgccgcctt cgatggcgta
    35701 agtcagtgcg gttggccatt tcgagaccgg ttccagaact tctctgaaac cgccccggct
    35761 gccgtcgatt ttgacgctgt caaacggcca cgcccgctgc agaatcagac ggggggcctt
    35821 tttgtcgttc ctaaagtaca cccctttaaa atcttcgttt tccctggcaa aaagatcata
    35881 accatccgct tcgctgcgag ttttcgtgta atccttgccg tccagatgga ggcccttgac
    35941 cgcgatcact tccacatcaa ttttctgttt ttgttcgccc ttgttttgcg gaattttcca
    36001 gtcgccggta atgaccgttt tgtcgccggg attatgccag cgatcggtga cgttgaagtt
    36061 ttgaatgttc aggcgataac gggccggttt ggaattgtct atgagttccg tcgcataatc
    36121 gaaaacctga caatattgtt tgttctgggg cgtgaattcc tccgccttgt attgaccggt
    36181 gagcgttgcc gtgactgcgg ccgggccggg ttgatcaaac cgcacggcag cgccgttgcc
    36241 cagaattttt ccttcgcttg tggtcagacg aaagccgatc cggtcggacc aggcgcgcgg
    36301 atcaatgttt tgctgaacgg agatgtattt ttcctgagcc gtgcggatgc attgggaacc
    36361 ggcaaaggag ccgtcgttat ttttcaaaat tttgtattcc aggcaacact tctcgacagg
    36421 gtgcgactgg ctgttgacgc cgatgatcga agcggttgcg gccaaaggaa gatttcgcgg
    36481 cagacgcagc agggcggagg tgagctcatc cgtcgccgcg ttggcggaaa agccggactg
    36541 gttgcctgca ccggagaagc ttaaataccc cggataaaag gtccaggtac cgggcacggt
    36601 aggcgccggt ttgatttctt caattttttc cggctgacga aagctgaact tcagtttccg
    36661 gggcctgggc agagattgga gtatgaccga cggatcgatg gggctctgag ccagatcggc
    36721 cggtccgcgg ctctgccggt agcggcttcg ggcggcggcc aggcgcttgc gggcggcagc
    36781 caggttacga tcgacaaact cctgcggcga ctcttctttt acctgggcgg gggcctgatt
    36841 ctgctgggcc aaagagcgcg gcggcatcat cagcgcgcct gacccgataa tcaacattaa
    36901 agcaattaat attttgatat tgacagataa ttgcttcatc ggccaccttc aaacgtgagt
    36961 cccagcttct gacaattttt ccatccttcg gtaatccagt cggcctgaat gccggacaga
    37021 atcttttcct tgatctccag acagaggatt ctttcctggg cggcctgaat tccggcaacc
    37081 gtctgcacgc cttcccgggc cgtattgacg gatgtccggg ccgcggtcgc agcggcggac
    37141 ttctcgacaa tatccgcctg aacaagcttt tgcgcccctg catccagttg actgttgatt
    37201 tgttccagcc ggactttctg ggcatccaga gaagcccggg ttttggcgta ttcaagtttc
    37261 ctggcctgct ggctggcttc agcggcctcg gaaacctccc gggtaaggac atcatcggct
    37321 tttcccgatt cccgggcgta gtccccggca tgggaatagg cttgaccctg gggatttttg
    37381 ggtgaggaca tgcgataacc gctgtcccac gtattgacca tctgctccgc ctgcggattg
    37441 atttttctta cgcccatgat gtcgtcgacc tgatccagca tgctttcagg aattccttca
    37501 tccaccatat tctgggaaac ggctaacatc ttgccggggg cgccctcaac atttgcttcg
    37561 actcctttgt cgtatgcgcg ctgcatcttc cgggcgaaat cggctttgga catgttgacg
    37621 cccgacggga gatccccgac tttatagaag ccgcccgtat ccagcggcac gacgcgatag
    37681 acgcccttgg cctcatccac cgtctcaaag gcgaagttgc tcagtttgtt gtccgtccag
    37741 acatagcctt tcgcgttcat gtccctcata aaggcctcgt aggcccggac atgatcggcg
    37801 gtcagctttt gcgccccggc cagcttgtct gcgtgcatgg aggagggtat gtgttccatg
    37861 acatccacaa ccttgtaact ggcgccgtcg gggatctcag ccaggttgcg cacggttttt
    37921 gccgcaccgg atgcgtcctg cacgatgtaa taccgatcct tcacctgcgg aaggcgtccg
    37981 atcgatgaat cttttgttat atcctgaaga tagactcgtc cggcatcgtc gagatcacgg
    38041 gatttgcggc tcaaaaccgc cgccggagtg tcgcccttga tgatcggctg gttgctcact
    38101 ttcattccca gatcttcgtt gagccgcacg taggcgttca ttcctccctt ggtgtcaact
    38161 gttgacgcat cggccagcag gtcttccggc gtgcgcagca ttttggggtc gtaggggata
    38221 tccctcgtct tgccggataa atccctttcc agcttcatca gcgcatcttg attttttctg
    38281 atgtcgtcgg ccagatgttc gacggtcttg acactgggaa catcatctaa agtcttgccc
    38341 gcccgggcgg ccgcttcctc aagctttttg gctctggcgg cggcctgcga cagatccccc
    38401 gccgtgtccg ccgcctcggc ggccagtttt gcggcctgtg aacccctgga caggatgacg
    38461 cccgtggcga cgtcggccag aatgctgccc acgagcattt ccgtgttggt cagcatcttc
    38521 cgggccaact gcgtttcttc cgctctctgc cgccagtctt ccatcgtctg gcggtcataa
    38581 actccatccg gcggatcatc gataagcagc aggttgaagc cggagccgat tccccggaaa
    38641 taatcggcaa tggaaccgac tcccttttca atcagtgcct gcttggcatc cgaactttcc
    38701 cagaggtaaa ccatgtcatt cttggtttta ttgagttttt cataggcttc aatcggatgc
    38761 agttgggcgt agccgatgcc gagcaatata ttgccgacag acccgaaacc ttcgcagacg
    38821 ccgtgaaata cattgaagcc ttctccgaca aggagtccgc ccgtcccttt cagaccggtc
    38881 aggtaggttc ccatggcgat gatggcgtct ttttcggcct ttatcttgat tttctggcat
    38941 tcgatttccg tcttgatgta gggcagcgga tcaaatttca gcggcaggtc caggggaaat
    39001 tcttcgggga tatcggtctg cgccgccttg atcctgtcga aaacagcggg tttgggcatg
    39061 cggaaaccca tgccgggcag agcctccacc gttccggcgt cgcccgcctg atatattgtc
    39121 ggcgggggag aaaaaccgga attttcggaa aaggccgtgt atgtggagct ctccacgatt
    39181 tcgcccaggc agtgctcata gctgccgctt tgcgccgcga tggtcaaagc ctggcggttg
    39241 ctttcatatt ccctgcgcag cgtgtcggtc atgaaagcag cccggcggtc cagttccgaa
    39301 aagtgatcct tgtagggatc ggcttccatg gttttaaagt actccgcccg cgcggttttc
    39361 caatcctctt tgagccgaat catttctttg cgcagatgct cggcgccggc gcgctgtgct
    39421 tcaacgaaaa acagcttatt gccgagatct tcctgctttt ttgcccaacg gccgacgctg
    39481 gcctcgctgc cgtaatatcg cctgtcttga tcggcgtcat actgccacac gcgaacggcc
    39541 atgtccgcca tgttcatata ttcggggaca tcttcataag atgcttccct gagcatcccc
    39601 tcgcggtcgg cctcctggta aagctgatca tagcgcgcgc gaaaggggcc ggcggttgcg
    39661 cctgccttat cggcattgtc cgtcagaaaa ttggccagga ccaggcggtg ggtatccaga
    39721 atgccgcggt agagcgccag gcgctgtcgt tcctgatcct ggtaagtctg tcccaggcgc
    39781 accagctccg tcatgtacgc cagcggcttg tcgcaggggg tcaacagcag cgtttccaga
    39841 cggcggattt cttccagctc ggcgttcgcc gccgcccctg aaggaacgaa ctgcgcaaag
    39901 gcaatcaaga gcaaaatgaa gaagattctg gaaaaaagtt tcgtcatgat cagtgcctta
    39961 tcgcggcagc gtaaaagccg ccccctgtcc gtcgtggact tgtgccgccg tcttgatcag
    40021 ttcgtcgcgg gccgtcaaag cggccgacag cgcctggctg atcgaacttt gcatggattg
    40081 aagatccgcg acggaatagc agtcaatcgc gctccagacc tggtaggcgc cgtcatcctg
    40141 ccaggtttcc agacgcagtc tgtcggccag atggctttcg gaaagcatcc gtttggaaat
    40201 atcaacaccg aagaaacgat cgggcttttc ttttaaagat tggtttaggt ctttttgcag
    40261 gcggttgagg tccgcgttga attcgccgat atttttggga aaccggttga gggaaacggc
    40321 catggtggcg gtaacttcgg gaagagaaag cccctggaag acggccggcg ccttgagagt
    40381 ttttttgcgg ttttcgtagg agttttttaa tccggcggaa gcgtttttaa atccctgttc
    40441 ggccagacgc aggcgataca ccagatcgac ggttttttgt cccgcttcgg cgcccgacgt
    40501 tccgcaggcc cagccctcga tggtcatggg tggaacatag gaagagttga gctcgacaaa
    40561 cgccgattcc gccggcgctt taatcagcaa gtcttcaccg gccccgaccg gcgcagcggc
    40621 ggccagcaga atgagaatag gcaggaaagg aaataagacc ggaaaagttc tttttccggc
    40681 ccgaaacgga atggagaaaa tttcagcaag cgaagtacac atcaaccgta agaatgtgct
    40741 taaatgcctt ttatgacttt ggcaacctga ttgtccatgc aaccgtgcaa ccgccggaac
    40801 cgggaaaaaa ccattccctc gcttggcatc gcattaaatc atcggatttt ggaaatcaag
    40861 tgtttttttc aaggtgattt tgctgaattg acaggcggac tgattttatt tatggttcat
    40921 tcggttttca aaaatcatgg acatcaacat caggagaatc tatggaacgc ttatggaaag
    40981 gatgtttgct ctgcctggcc gttcttcttt ttgccggctg cacggcggtg aatcattatc
    41041 tcgggttctg gcaggctaag caggaggcca gggaggcctt cagccgggag cagagcgcgg
    41101 agctgctgat gcagcttgcg ccggaggatg cctatcttct tctgggcaag ctcgcttttg
    41161 accgggaacc cgcgggtccc atccttctgc tggcgcttac ggacaaattt aaaaaaagag
    41221 aaatcgccgc ccgctctccc atgcagacgc ccctggatta ctatcagatc catttgcccg
    41281 aaggtgttta tgaaatttat ttcttcgccg acctcgatga aaacggttat ttcgaagcgc
    41341 atgaatgcgt gggccgcctg cctcggccgg tcacgattgc aaaacaaaaa gtcaaagacg
    41401 gattgtccgt catcggaccg gatttaaaac tcaatctggc cgctccttcg aaaaccgatc
    41461 tggttttcga agaaaaggtc cggcggcatt cctatgttta cgaatcgctg gaggatgaat
    41521 tcttcgatcc ccgctacggg gagatcggcc tttatgattt caaggttttc atggcgcata
    41581 ctcagcagta tattttttcc tttgagacga tgaatcctcg aaagacgcag gtgctctttg
    41641 tgcacggcgt cgcgggcacg ccgagggatt ttcaatacat ggtccgcgga atcgaccgga
    41701 agcgctacca gccctggttt tatttttatc cttccggcct gcccttgaaa aaactgggcg
    41761 ccaacctggc caacatcatc aagtatgccg aagcgaacaa tcccggccgc ctgcgccgcc
    41821 tcatcatcgt cgcgcacagc atgggagggc tggtggctct atccaccatt cagcatttgt
    41881 ccgctgacgg cgttccgccc tatctcaagg gctgcatctc cttcaactct ccctacggcg
    41941 gcgtcgaggc ggcggcgtcc ggcattaagt atgcgcccgc cgtcgtgcct tcctggcatg
    42001 acgtggcggc gggaagcccc tttctgaacg agctttacac ggaagggaag cataaggcca
    42061 ttcccttcta cctgtttttc ggttacaaga ccggcgacag cagcgacgga accatcacgc
    42121 tgcaaagcca actgaacagg aatatccatt tatccgcgca taaaagttac ggtttcaatg
    42181 cctcgcatgt gggcatactc cgtgatgaac aggcgcgtca ggtgttttac aggaccttgt
    42241 ccgatctcga ccgtgactga cggcaggggg gcatgaggca tcgccccggg cgcttcatga
    42301 tgttcaggcc ttccccgtct tgttccgcat cggcgcagtc cggacccatt cccacgctcc
    42361 agcaacagga gccgggcgcg atcatccggc tattttattt cctccagact ccacagggtt
    42421 tcgctcaaag gcgggaagct ttccgcaaac tgaatctcca tgcgtacctg gccgtcaagc
    42481 cgccggggca tgccgaaacg gatcacgccg ccggggcgcg accactcggt cagacgaata
    42541 aaacggccgt ttcgaatatc ggacaaaagc cggcctcccg tctctccctt ttcataaaaa
    42601 ctggacgggt tgccgtagcg atccagaaga atggacagaa tcctctgaaa cgtctgcata
    42661 atatcatcag gctgctccag gtaggcaaaa tcgatgatga cggcggaccg gttcaatgca
    42721 ttgttttttt gaatcaagac cagccgctga cgcaggtcta tttcatcaaa cactctggtt
    42781 tcgtaaagga gaaagttttc ctggcgcacc gggttgacaa tgcctttggc gattaattgc
    42841 ttttcggcct cgtccatttt tgcgcccggc gcgaaggaag ccaaatcaaa tgctttggcc
    42901 ggcatttcag ggatttctct tcgttcttcc gccgctttgc cgcgccccgg agtttttgcc
    42961 ggtttatcaa aagaataagt ccagaatgcc gccagtttat aataatcgat ggtctggcct
    43021 ggatcgggat tttggtagcc ggaggaaagt tccagaccaa aggaatggtt gttcagggta
    43081 aagcggtagt tcagggcctg ggtggtagtg atcatatcga tgccgtcggt gacatagcgg
    43141 cgctgatccg tccggctcag ggtgtaatcc aggctgtgcg gcccccgcgt cagccggaag
    43201 gcaatagtgg gagaaacgtc aaaggaatct ccggtgtcgc tgttgatttt tctgagcacg
    43261 acacccggcg tgaccgcgcc ccggcaaccc aaaaaatcaa gcaaatgatc cgcgcttagc
    43321 gaaaattgat gggaagcgct gtcccccgca tcgggaagac ggttgaaaaa attgagatac
    43381 tggtagccca gccgtcccgt ccagccggca attttgggaa tattgacatt cagatttaca
    43441 ttgtgtgtta gattgtcgat tgaatccgtc cggtttttcg catcctggcg gaagacatcc
    43501 agtgatccca aaggcccggt aagattcgcg ccgaaaagag acgcatccgt cggattggcg
    43561 cggccgagat catttcttct gccctgggcc cgcagccgca gttgataccc cctcgcaaaa
    43621 agccagcccg catgtccttc caggttgcgc aaggccggtg taatggacgc gcctgtcggc
    43681 tggtagtctt cgccgtattc ttcatagcgc agccggtaag tgaatggatg tttgccggag
    43741 ccggacgcct gaaaaaaaat ccccctgtcg cttttgttct ggccgctttg cgcatccgct
    43801 gcgccgtcat gatctcctcg aaacagtccc agctcgcctt ccagaagcag tttctggttt
    43861 ttcgcctgaa gcgtatgctg gccggttaag ctgtaaacca tttgagtgcg ctggagaatg
    43921 ttgcgggctt cgtcgctttg acgcgtgttg tatgcaatat tcagactgta gcggccaaac
    43981 gtcgggtttt cgattaaata ggagccgccg ttgaaatagc tgtcgcggaa attcagatgg
    44041 cgccaggagg aaagacgggc gccggaggat aaaagcacgg aatgcctgga ggccgagccg
    44101 gcaaaaggct gcagatcaac ctgaacgccc ttgagagacg tctgcagggt tctgtaggtg
    44161 tagtaggaat acacatcgcc cagttccaca cggaaaggca gagcaatgtc tcctttttcc
    44221 ctcatgagat ggatgcgttc cggaaccagt ccgtaatcgg cgctgcggta gggagacccg
    44281 ttgatcacgc caaatatatc ccccttccag agatcatagg ggtttttgcg cctgctgaaa
    44341 ttgaggccga tttcatcata ggaggtcagt ccttcataac tgtaaggggc ctgggcttca
    44401 tccccttgat aatcgtagta ttccagccga aaagtgttgc tgccggatat ttgccagtcc
    44461 ggaagaacgg catttgccgc tctttgcagc gcgccttccg tttgggcggt cacgtcggcc
    44521 ggcgcaaggc agataaacat catcagaaaa acacaagcca gccgccggac agagccggtt
    44581 attccgccgc cgcgacgcgg gatgctttga agtctcacgg aatctctcct attgcgtccg
    44641 accaggacga aaccctgcgc gatagagcgc gaaattcggg gaagagcgcc aaatgcccgc
    44701 ggcggttatt gcgccgcctc ctccgtaatt ctgagaatat attcattgac cccgccgaat
    44761 ttaacaaacc cttttgtcgg atcattatat tcaatctgcg tgtccgccgt cacggaaacc
    44821 tcgctgatca tttcatggcc gcctccatct ttgtttctct gaaactgctg aagcgtctga
    44881 acgccctgca cggacatgat tcgaatggct gcgtctttcg gaaccttggt gctgattgcg
    44941 tcggcaagcc cgctcttgtc gtaaaaatcg tcgcccaggt attgttgaat tccgccgccg
    45001 ttccaggcgc ccatcagctt cttcacggaa tcggcggccg cgcttttggc gatcggacga
    45061 aactcctcaa ccggggcggc gcccggagga attattccgg gtgtcggaat ggcgttgatg
    45121 gtccggaatt cctgcgcggc ggccggtgta aagcttatgc ttgcggcgag gacggcagcc
    45181 agcagaatcc gcggaaggct gatcggtttt gttttcatgc tttgtttcct cttacggcgg
    45241 tgaaacattg gaaatgaaca gctcgaattt ataattgctg atttttttca ccgtggcgaa
    45301 aacaaccagc ttccgccccg cgtagggtgc ttttttggac tgcaaataaa agcggccttc
    45361 cacatcgaat ttcaggggga tcctcacaaa tccggaaccg gtgggcggaa tgaattccgc
    45421 ataggcaaaa tcaggaaagg cggccacgtc cgtgtaggtg cagtccggag acagaagcgg
    45481 caccatgatg tcaaaggcca ggaagttcca gccgctgtct ccaccgacga ctttgcaggc
    45541 gcttgcggcg aaatccacaa aaagtcccgc cgaaaaagtc ggcatttgct tgcccaggca
    45601 ggtctggaaa ccggaggttg atttgaacgg cgccgactgt ccgatacaac cggcaaaggg
    45661 tgtcggaggc ggaggcggag gcggaggggg cggggagggg cctggaccag gcgctggact
    45721 gtcgccgcca ccctgcatca ttttttgcag ctcttcaacg gacgcaaaat acagccggcc
    45781 gttaatcgtg gcattgttga ccggttcgat ctgattgagc ggattgttgc cgcgcaccgg
    45841 aataacccgt acaacctcga ttttggtaat tttcgttttt ccccggccaa gtaaaaccgc
    45901 gccggattcc gccagcgcat cctgcgcttt gcctgcctgc gaacttttat cggaagacgg
    45961 caatgcgccg acaaagcgct ccgtcagctc ggccggaaaa agcggccggc tgtcttttcc
    46021 caaaatgatc tcttccgcca gaacattttg aaaagagaca aataaaaaca aacaatttaa
    46081 aatcatcaac atccgttgaa acatttttta cctccgaaat tcatccgcgc tgcccacgta
    46141 cttttccggg tcaacgaaca gcgggtagtt cggcatatcc gtatccagag acagctccac
    46201 atgaatcatc gcggcgggat caaaatattc ctggattccc ttgaccacgt taaaacagcc
    46261 gcccagcatt tccgaggaca tccggacggg cgtcatgtct tgttccagat aaagtttgct
    46321 ctccgaggag atgcgccacc ggcaccatgt tctctcccag aattcccggg aaagtccggt
    46381 aacggccgcg ccaactttca tggcgtcgtt aaacaccacc agatccagac cccttttctt
    46441 cagctgttcg gcaaaacggt tttgcgcccg tttattctgt gcttcctgcg tctgctgctg
    46501 aagcttttgc cggcagtagg tttccggttt tccgcccgtt tcaaagcccc acatatccgc
    46561 ccagcgggca accgtgctca cgcagtcggt catgctcttg aaatcattgg aattgtcgcc
    46621 cgtatctttg ccgacggccg cagcaggcgg acgagtgggc aataatacga tattggcctg
    46681 ggccagcgtg cttttcgcgt ccagctgggc cagcgccaaa tcatccagaa caatccggac
    46741 atgatcaact ctgctgccgg acaataatgt ggccacgaaa gcgtcgccgt ctttggtaaa
    46801 ttgcgtggcc gtttccgacg ccccggcaat tccggctgtc ttccataagg ctttgtactt
    46861 gctcatgtct cctgcgccgc tcgcggagag cttgtagagc gttttcccgt tttgcaatgt
    46921 ggaagataag atcaggcggt tgacattgac ccagatggtg ttggagatca gcttggcctg
    46981 agccgtttcc agcattttgt aattttcttt tggatcgctg atgttggcat agccgctgat
    47041 tttcgcgctg atttcggttt ccataggata tacggactgg cttgtcaaac gcccgcccgt
    47101 cgttacggcg gcggcgccgg gattggtgga tttaaattca atctgcacgg gaactttcac
    47161 atcgccaagc gccagcggat ctttgacgtt ccgaagatgc aaaaacggca tcaggcgaac
    47221 atcggcggcc agatgattgg ggtaaaacag gtcaatttca tccatttccc ggggtgtccg
    47281 gttaccgtaa cgaaccagaa gcttcagctt ttcggccacg ggcatcgcca gctttgccga
    47341 tccccggaag gagaaaacat cctgccgcgg cggaggagac aaaatctgca gcatcctcaa
    47401 cagatccttc ttttgcggat tgcttccgat gatcggcaga taacttgcac tggcggaaaa
    47461 atcgactttt ttgcccactc cctccggcca tttgtccaac acatccacaa tcagtgtatt
    47521 gacggccgac cagacattgc cttctttttt aaagtaggtg cgggattctt tccattttgc
    47581 cggtccggcg gaactccagt taactgtata gtctccaccc atgtcggctt ctccctgaac
    47641 catcagtttt acggaatatt ctccgccctg tgccagttct ccatcacgca tttcgggcgc
    47701 aggctcaacg gctgcggaat tgaccgtgaa acggtgttga accatccgct tttctgtttc
    47761 cagcttgccc agattccagg caatgacact tgcgccagtg ccgcccgcat actggagaaa
    47821 tccttttccg tcaggcagaa tcgggcctgg tgacttgacg tccgtatgca cggtgacggg
    47881 aaagtcggat atttcaaatg gtttttcctt ctcctgacct gtgtcgataa ctaaaatggg
    47941 actgattcgg ccaatcgcat aaggatcggc ccaaatatcg agcatgaacc gttcaggcgc
    48001 agaggtggaa aggcttttag tgctggcaat gacgccgttg taactttggt tgacgggaat
    48061 aaaatagtcc aggaaaggcg ctttggcccc cggaagatta aagtcgaggc ccggaatgcc
    48121 gaaatccttg aatttgatgg actgaaattg gatggttctt gtaaaatccc ggcctgcatt
    48181 gacatagtcc gtccattcat ttcggccctg gcaaagctcg gggttgattc cctgcaatcc
    48241 gccctttccg gaaatcgtca ctgccggcag gccgggcgaa gaaaatgcaa ccgttccgga
    48301 atccagaacc gttgcgccgc ccgccgcgct caccagcacg cccagcttgc cccgcacatc
    48361 ggcaggcacg ctaaagacat tggcttcgcc gccggataca gcacgccgca tgacgccgca
    48421 aaaattactg accagagaaa atttatcctg caagatggaa aaaccgattt tgggcgaaaa
    48481 accaatgtcg gtgggaggaa tcctgttttt aagattgagg agcgcggctg tttctgagtc
    48541 tgccgccaca tagcccggat aggctttctc aaaataggag atccctccca gaaccggtgt
    48601 gtatctaggg ttgcgcaaat cgctgagagc gtccgcgctc agttgcggca gcttttcttc
    48661 cgggtaggca acgttgaaac gcagcgtttt gggttttggc agcttgcctg caatttcctg
    48721 cgtattgcca cccctggaga tggactccaa aagcagggag ccgcagttct tttcataagt
    48781 ctgctctaaa tttcttaatg attctatggt gttatgaagg ttttccgcga taaacgaggc
    48841 gggctgggaa atcgtcaact gctcagcctt ttttcccttt tccggatctt tggccaaaac
    48901 ctggtttccg aagccttgca gcagcaaaaa ggtccaaagc acaagcgcgg gcaggatcac
    48961 gaaataaaat ttacctctga acatgctttg tctcctcatc gacctaatga ttcagattgg
    49021 cgcagggatt gcccattttc ttaagctttt ccggatcttc gccggcaatc agcagctttc
    49081 tgtatttcac gcattccgca cagtcggaca gtctggaggc tgcattggtc atcaaagtgt
    49141 atcctgccgc ctgttccagt gactcagcta tttccgcggc ttccggaacc ggggaagcct
    49201 tcttccttgc ttttattgcg tcctcggccg gagcggctaa aacggcatca gccgttttcc
    49261 cttgcaggga cactgccagt tcctgatcag tcattttagc aatgtttccc gtgttgatgc
    49321 gaatgtctcc cgccgccatg ccgatgctgt ccgagctttc aatgcccatg gcttttatat
    49381 cgaccagcgg cttttcataa gcgccgagaa tcttttcccg tatttccgcc tgctccaaaa
    49441 aagtttttcc ttccatggtt ttgcccagtt cgttgacccg aatctgcatg gcccgggcgt
    49501 ttttggccag ctcttccgca gtgtttcctg taactttcac gatgttgccc gtatcaaaca
    49561 ccaccacgcg ccagcgatct tcacccggca gtttttcaaa ggcaaaattg ccgctgtgat
    49621 tgtccaccca ggcaaatccc ttgctgttga tgtcgcgcaa accctgttca taggccattt
    49681 tttgcccttc ggttaaaatt cctccctgtt tttttattgt tgtcgccgcg tcttccgcca
    49741 ctctttccac tacttcaatt ttttttgctt tttcgatgcc caattcgtcg cccggtgtgg
    49801 tgtaaacagc ttctcgttta gctacgcgaa gcgagtcgct tttaacgtct ttttccagag
    49861 cctgtcggcc aaaatcatca agtttggcgg cagcaacggc ttttttgtcc agcacggttt
    49921 gtctgataat ccgcgaatca ttggtcgcgt cgataaacgc cgtgctggtg gaaccttttc
    49981 ccagttgctc acccagagtt agagaaacat tttgtccctc cgctgttgag acctgaaccg
    50041 tcgctttggc ctttgtcggc tggccggcaa aaggtttaac caccggttca ttgacaactt
    50101 tcttttgcaa tatttccagc ttaccgttga cgtctttttg tccttcaata attttttcca
    50161 gacttttggt ttttgcttcc acttgcgcgg tcagggcggg attttgcgct ttcagggctt
    50221 tgagctgatt ggccagagac tcgaatttgc cgcccgcaac accggctttg gccagcgaca
    50281 ccgcttgccc cgttttggcg ataaccgcgt ccgccttcca gagcatggcg atttcgccgg
    50341 taactttggc catttccttc aggccaacat ttacctgccg ggccgattca atttgctcca
    50401 gcatcttttc cagttttttt tggggcgggt cacccggatt ggcggtaaaa cgatcagaat
    50461 caacggcgcg gagcgccaac tgtgaaccga ggttgcccag tttatcggcg gatgattcca
    50521 gggctgtgcc aatggcttct ccaatgccgc cttccgcctt gattttgtac aggtagccgc
    50581 ggtcgagaac tttatcgccg aattcgatca ccgccttggc gccgttgaca gtgctgtcga
    50641 tgggatgaga agcgatatcg atcatcgcgt cgcccagggg cgcggttgcc tccacaatgt
    50701 gaaaggcggc gtcaaatgcg aaagacaaag tttcatcgac aaagtcaatg cccagttcaa
    50761 tcgtgtctct ttcctgtttt tccagctcca gcttgaggat ttcttccacg aacgcccgcg
    50821 ctttgtctcc gtcgcgcttc agatgaagct ggaacggaca atcgggaata ttggtcctgg
    50881 ccagcacggg cagattggtc ggttgcagaa actgaagacc cattcccggc aggccttcca
    50941 cggtgtcctt ctttcccttg ataaacagct tttccgttcc atcaatatat gtgtaggagg
    51001 aggaggcggg cgccattttc cccaggcagt gttcgtagtc gccgctgtag gcggctttgc
    51061 tgagctgatc tgcattgttg aaatagccgg ctttggcttt cgccattttc tcactcagag
    51121 atgccagatc tttattggcc aaatccagat cgcttttgat aaactcgtcg tatttctttt
    51181 tgtgctcatt ccatttgctc ttgatgtcgt tcagtttttt gctgagttcg gctttgccgg
    51241 cctcattttt ttccggattg ccgaaataga aataggattt cgcatcgggg gcaaaggcaa
    51301 attccgagtt gctgatctgg atgccggccc ttctttgctt gtcggaccag ggcggcgtgc
    51361 cgtcgatcaa aagtccagtc tcccgcgctt cgttttttaa tgaaacaaaa aggttataaa
    51421 actgacccgg ttctgctttg gcattggcgt cgggcgagac cacgaaatct ttcaggatgc
    51481 cccactgttt ttgattgtaa agttcataga tggaaagccg ttggttttca ctgctgtaat
    51541 aggactgcca gtgatcgatc aggccatcca tcaaagattg cacgtcctgg caggaccttt
    51601 tgattttctt gtcaatatcg ttgatctgga ttttgaggtt tgcgcagtcc gttttttctc
    51661 cttcctgatt tgcggccgga gaaatccccg caaaaaagga aaagaaaacc aaaaacagga
    51721 tcatcaatcc ggcaaatttg tttttcatgc ttcgcacttt cgctttttcg ggaaaaaact
    51781 aacgtttgat attattcaca atgagatgct gcttaacgct tccggtattt ttccacgtcg
    51841 cgtctgagct tttgatactc atcaaaaagc gcgatcgctg tctgataagc ctgcgtctgg
    51901 tttgagacaa acacatccag atcggatttt gtataacagt tgacgatgac ataggtttca
    51961 tcgttgtcca tcacctgttc cagggttgtt tcataaccca cgaacttcgc ggaggtatcg
    52021 gccaggcggt tgacgccatt ggctgctgct ttctgaaagt cttcaatctc cttatgaaat
    52081 ttttgaatgt agtaccggtc gatttcttcc atgctgcggg cggaaatcgt tctgtgtgcc
    52141 tgataatcgg tcagggtcag atcaacggcg gccgtccggg acaaaattga ttcgctgagg
    52201 cgggacgcgt tttccgaaag ctgattggaa ttcagcccct gctgcctgct ttcctccata
    52261 agcaaaccta gggattgaat gccttcccgc agaagttggt ttttgtcgac cagttcttcg
    52321 gaaatcaggt tggcggtgcg gatgtcgtga atgatttgcg gagcaaggtt gtcgccgcag
    52381 gcccatcctt ctacagcaaa ctgccgaaag gaacgatggg gataagaacc gctggccgat
    52441 tctttcggcg cggtgatttt tatgatgtct ggcgaaaggt tttcacaaaa ggcggcatgc
    52501 ggaaaggcaa aagaagctaa aatcaggact cctgccatga cttccgcaac tttatttatt
    52561 ttcatgttta ctccgcccag gaaacatgat ttttgatttg cattatagtc ttgccatctg
    52621 cgcaattcaa gaaaatatta aatacagaat gacttttttg gcctccgctc aaaattaaca
    52681 tcaaactctt gtcaagtccg gcctttttga cggatgcatt gtcaaccgtt ccgggctttt
    52741 gtctttctgg tcggaacggc gttccagggg tcgtcgggcc agggatgcct ggggtagcgg
    52801 cccttcagct cttttttcac ctgcggataa ccggtgttcc agaattgttt cagatcccgg
    52861 gttatcgaaa cgggccttcg ggcgggcgaa agaagttgga gaagcaattt aattcttccg
    52921 ccggcaataa ccggggtgtc ggcaagaccg aacatctcct ggagtttgac ggccaaaacc
    52981 gggatgtccc cggaaacata atcaatcggg atgttatggc cgctgggggc ggcgatggtt
    53041 acgggggcac gctcagcaag aagacgccgc tcttcccacg tgagcctttc cgtaagcgca
    53101 ggcagcatgt tcaggtcggc aagctgctgg gcgttgcgaa tgccggaaag ccggggcaaa
    53161 agccagtcct gtggcgccga aataagcgtt tcggaagaga tgtccggcca tttttcttcg
    53221 ggaaaggttt gttgcatcaa gcgcacccgg gcctgaaact gcaggacctc cgggctgaag
    53281 gcgatcataa cggtccttga tcggatggcc tcgcaaagga ctgaagcgac ttcctccttg
    53341 ggtggattta cgggtttggc cgaaaggatg atcgtcccta aacgttcttc acgggtggcg
    53401 ataattcgtc cctcctgttt gtcccaaacg acttgccgca cagtttcaat ctgatcttgc
    53461 agtgcctcac ggatgagctc ttccgtcagc ggttcagcca tatgcaccat gccttccgcc
    53521 ttttcgccct tgtccaccac aacggcaacc aaacagggac tcccgcccgg ggcaattttt
    53581 gctgacagcc gaacgcctct tccctgccgc aaaacataac ggctgttgtc gtcttcgcga
    53641 cgcttggcga tgcggtcggg aaaggcaaaa agaagcagac ggggaatcat gccgccgttt
    53701 tcaacgcctg cgtcttcaga ggtcttgccc atcaaacgcc ggagctgctg cgatgtccta
    53761 tctaccgcac gcaaggcaaa agcgtcggcc gttttcggca gcatcttgtt ttgccgccat
    53821 tgcccgagga gaccgagacg atcgcttaga tcaaacccgc cggctgaatg cggaaaagag
    53881 gaccgggaat gacggatgat gtcccgctcg gacagcagcg ccgccagatc cgctccaaag
    53941 gacggacagt ccaaatcagc cgctctcaaa agcaggcggc cgaggcgcgg atgaagaggc
    54001 aggcgcgcca tggcccggcc tacgggtgta atcgaaccgt ttgcatccag ggcgccaagt
    54061 tctttgagca attcctttgc cgcgcgccag gccgaaggcg gcggcacatc gagccaggaa
    54121 agcagagcag gatctttaac accccatgcg gccagatcca gaaccagcga tgacagatcg
    54181 gaaatggcga tttccggcgg ggcaaaggga atcagggact ggaaggtatg atagctatat
    54241 agtcgatagc aaacgccggg acccagacgg ccggcgcggc cctgacgctg cctggctgcg
    54301 gcctgtgaaa ccgtgacggt gaccagacgg ttcatgccgg ttcccggatc gtactcaagc
    54361 ctgcgggtca atccgctgtc aatcaccgca ccgacgccct gaatggtcag gctggtttcc
    54421 gcgatgtttg tggccaggac aatttttcgt gcagaggctg gaaggatcgc ccgttcctgt
    54481 tcttcaaagg gcaaatcgcc gtagagggga tgaagtgaaa tgccgcttgc cgctttgcca
    54541 tgaagcgttt ctgcgatggc atcacctact gtgcgaattt ccccggcgcc cggaagaaag
    54601 acgaggatgt cgcccggcgt ctccctgagc gctctttgga caacctcgtc cacgcgcgcc
    54661 gcaatgtgtt tatccgggtt gtcgccgcag tagcgctctt ccacgtggta ggccttgccc
    54721 tccgatgaaa taatggctgc cccgtccaga agggctgaaa ccggcccgcc gtcgagtgtg
    54781 gcggacatta cgaggatttt gaggtcttcg cgcagggcgc ggcgaatatc gagacaaaga
    54841 gcaagcgcca ggtccgcctg aaggctccgc tcgtggaact cgtcaaaaat caccatggcg
    54901 acgcccgcaa gatcaggatt ggccagaatg cgccgcgtca ggattccctc cgtcatcact
    54961 tcaatgcggg tcttcttcga gacccggctg tcaaagcgga tggaataacc cagcgtttgg
    55021 ccaatctcct cgcccagtgt tccggccatc caccgggcgg ccgagacggc ggcgatccgc
    55081 cggggttcca gcatgagtat gcgtccctgt tcaggttcga tgatatccag aagcgccagg
    55141 ggaacccgtg tggtcttgcc ggaaccgggc ggagcctgaa ggacaacggc gggatgttta
    55201 cggatagccg ttttcaattc cggaaggatg tcgtcgatgg ggcagggttt taaaatttgt
    55261 ccgctcttga tgttcttttg catggcggga gtatagggag ggcagccgat cctgtcaatg
    55321 caatcgtgcc tcatcaccat ggatttgatt ccactgatga aaataaaggg atgattctgt
    55381 tatttttacc agatcggctg gtgggctttc ctgccgtaaa cgcgttcatg ggctttgcag
    55441 aaggggcaaa gcccgccttc aatctttttg acaaaccaga acgcggcgcc cttttgtttt
    55501 ttgcgggcat gtctgcacac ggggcatttc acgcagacca gcgccatttt gcgatccctg
    55561 tccgtgattt tattttccgc ggcttttacg gccgcctttc cagcggtccc tttatttttc
    55621 tttgcggtcg cttttctttt tgacatttac atccttcctt gctgttgtcc cggcgttttg
    55681 gcggggcgat gatgtaaatt atagaaaaca atggataaaa gcaataaaaa gtttgaaaat
    55741 aaaaagtttg aaaaaaccct cgaaagttgc tagcaggcaa taaccgttct ttaaggagac
    55801 agattatgaa aaagatgacc tgcctgctgt tgatttgcgc ctttctcgcc tgttcccaat
    55861 gtgtgtttgc ccaggattcc gcggagaaaa gcagtccgaa aacaaccggg gaatatctga
    55921 agaaaacggc aaaggatcag gcggcatcaa cggcggaaga tatggctaag aaaaaggttc
    55981 aggaaaaggt tgatcagaaa gcgccgcaat tgatcaattt ggcatacgaa aaatatccgg
    56041 tgatcgccaa attctccggc aggaaagatt tctcggagtt aatgaaggat tacaacaaat
    56101 acagtgacgc atatgaaaag ctcaccacgg tgaattcgct gctcgtcaag gtggcggaag
    56161 gcaacgtggc gggcgcgacc tatgacgcga ccaaagaaat attcaccaaa gtattccctt
    56221 attccaaata catattttca tatctcgaag cgtgggacaa aattcttacg tccgtatcgg
    56281 acgccgtcat ggaaaataat cttgattaca tccgcaagga attcatgaga gacgtcctgc
    56341 cgaagtgcaa agaatcctgt ccgttgtcgg aggagcaaag tttcgccgga agaataggcc
    56401 ggcgcatggc ggaagatgat cagggcgctt acggtctggt tgcctggtat tgcaaggagg
    56461 gaggcgcggc cggcagttcc aagtgcggaa gcatctgtgt gtccgggccg tttgactggt
    56521 ccaaaaggag aagctttgaa aactcgggat gtgaagatta tgccgtcatg tcttttgtcc
    56581 gctccgacca gaagatccgt caggcagtgt ccgctcacta ttccattctc cgagccattg
    56641 aggatgtggg cgatatgcag aaagcgatga aaaatgcatc cgattatgta aagaaaaatc
    56701 tgcttgtctt tgaacaggag cagggaaaaa tatttgccgc ccaggaggca ttaaaagagc
    56761 agcaaaagaa ggccgcgcag gaaaagatcg ccatggccga gccgccaacg cagtgcgatg
    56821 aaaaatgcgc ggagagttat cgcaaagcct ggtataagac tagagagatc tcgcaggaga
    56881 ttcaagacaa gcgcagcgcc atcaatgcaa gggtccagga aatccagaaa gaaaacgccg
    56941 ccagaagaaa cgaactgaaa ataaatgttt catccggcaa tttggaagag ctcaagaccg
    57001 atcccggcaa tgtatccatt gacgaggaca acgttgatta caatgggcgg caggcccaaa
    57061 ggttggaaaa gcttgtggcc aacgccgaaa aatggcttca gtctcagcag ctggagattc
    57121 gggtttacga cgggcgcata tccagccttc aggggctttc cggtctgtat gcgcagaaac
    57181 agaatctcga gacccagtat ggaacaggcg gcacttaccg atccagtgag aaatttaccg
    57241 ccaattattc gctgattctg gaaaatgaga tcaagggcgt ggagctcaaa cggcaggaac
    57301 tggtggatga aattactgtc gttcagaaca atctttccgg ctacaggtcc ctccaaagca
    57361 agtataaaag cgcctttgaa cagggcagca agaaagccag agaacttctg gaaaaagagg
    57421 tagagcccgc ctatcaggaa tatgcccagg ccgaccagga attggaaaat atcgaaaacg
    57481 aacgtcgtga atacgagcag gagaaactga tccagatcaa cgtggtcatg tccaaaatca
    57541 ccgcggcaaa gaaccaggcg gaattggaga ccctgctgcc ccaggtcaac aaagatatgg
    57601 ccgaatacaa tcagctgctg cagaagcagg cgcagctgat gcagaaggcc gcagacaaaa
    57661 gtcaaaaagt tgaagcgctt tccagatctt ccaggattca aagggcaaaa ggtacgctgg
    57721 gatatcaaag cagctccctg tcttcccgaa tggtccggcc ggacttgagc ggttcaaagt
    57781 tcagccttgc cgcttatctt ccttacattg ccgaggccaa ccgctatgtt gtgacggaaa
    57841 gcgcgcggcg ctatggcgaa accctgaaaa aaattcccga tgaagcggca ttcatcatta
    57901 tgccggatgg cgatatgaaa aagctttcta tggaagtgtc ttcggcaacc ggagaaaatg
    57961 cggcgagttc catcaaagcc caggcctcaa ccattgccag aatcaaagac ttcagcggag
    58021 cctacagcgc ctatcagtcg gatcttggca gttctctgaa ttctggaaac tggaaaatag
    58081 atgctgtgtt atccgaggaa gcaagggcgg ctttgaacag gctggccgct ttttggagtg
    58141 agcattttaa tccgcttatg caaaaacgtt cgcaaaaaat taatgagaaa attacctaca
    58201 gccagccgaa gatcggctcc ggagcgcaaa cccttcacgg cgccgtccgt ttgaccgccg
    58261 gtgacattac tgacggcaaa gtgccttttc aaatcacggc cagtggctgg ggcatctccc
    58321 gcgctcttgt cagagttgcc gcagccggga agaacctgcc gctgaccatc aagacctcaa
    58381 cggcagccga aatacccgag gctgtctata cggcgatggt tccggttgcg gccggcaagg
    58441 caacgcatgt ttcctacacg gtcggggact ggacgcattc cgttgatctt cgcgcgccgg
    58501 caggccatgc tgtttcgccg gaggatgtcg gcaaaattcg cgctttctac gacaaattca
    58561 aacaggaata cgagtcgcgc aacgattcac ggattgtggc catgatcagc gacgactggc
    58621 aggccggcga cggatcaacg ctttccgatc tgcagatgaa tctgcggcgc acctttcggg
    58681 tttttgacga gatccgctac aacattcaga atctctccgt aacacccggt caggacggac
    58741 gttacaatgt atcctatgat gtcaccatca ccagcagaat ttacaaaagg aacctcaagc
    58801 acgaagaaaa atcgagcgtt caggaagaag tgaccatcgg gagttccggc aagcccaaaa
    58861 tatcaaaaac gctgggggga aggttctgga ctgttcaata aataatgctg gaaattgcac
    58921 ggcaagtttc ttattataca gcaaagaagc gcttcaaatc gtgaggcggg gagattcaaa
    58981 gaaccttggt cggaattcaa ttttttctgg aaaccagaca aatctatgaa aaatccaaca
    59041 gtctgttttg gtccatgccg gtacatcaag aaaaacatga tgaagttttg catactcatt
    59101 ttcatccttt gcctgctggc catgcccggg ctttgcaaag ccgcttcaga agaagatgtt
    59161 tatgacgcca tgttcaacgc ggacgcctct ctttttacga caggctacgg ggttttcctt
    59221 gaccaggtaa aggacggcca gagatttggc gcttccatcg atgtgttcaa caaggccgcc
    59281 gggtttgccg ccacttacag cagcgtgacg acaaaagctt ttgccacagg cgatgtatcc
    59341 ggcgccggag aggaagccat gctggccgtc ctgtccgaaa tcgccggatg gccgtccggc
    59401 caaggcctcc tgaaatacgt cggtctctct acgggggtct ttcaggtagc attgacggct
    59461 tattccatca ccaaagagtc ctttgctcag gtcagggcta caaagattgc cctcaacata
    59521 gaaactctat atggctggat tgaaagcgac ccggtgctga agcccaaaaa gggcaggcag
    59581 ctcggagaca agagcgatcc gattccggtc aataatgagt cggtggaata catgttcagg
    59641 aaggtggcgc aggatgagag atggcgtaat ttgttcaagt cctatgtgtc ggaagaactc
    59701 ggagaaacct ttccagagcc ctcgacctgg gattggctca gcattgccgc tgtagacggc
    59761 tggaaaagcg ctcacacggc cgagcaggcg gaaatattca aggacaagcg gcaggtggaa
    59821 acatggatcg ccggtcttct gagccagttg aataaatatg ccagggctgt cgagagcgaa
    59881 gtgatcctgc ggcagacgtt ggcgaagatc gagaaggcat ccaagaagat aacgcccgaa
    59941 ttccagaagt ttgttgtcgg cgccgaggag gctgaaaaaa aacttcccga gatcgatgcc
    60001 tatgcaaagc aggttcccac aatcattgcc gctgcaaaga aaaatcaaaa ctgggacagc
    60061 ctctacgatg ttcgggacaa aatcgggtat tatgcaagag tttatgtaag agtgcttccc
    60121 gacacgggaa atatcggcaa gaagaagcag gcgacaaagg ccgccctcaa gaactcctgg
    60181 gtcgatgtgt ccaagacgct caaggatatt cccaaaacca ttaaagaaga ttttactgcg
    60241 caaaccgtga caccccaata caagacctcg atttcactgg ccgaggtttc tcctgatttc
    60301 attgacattg acgaggttgc cgcgagactg cacaaggcga agtctcttca ggacatggaa
    60361 gccgaagtga ttcttgtcaa ggaggataca gaaaaaaaat acaggacctt tcaggagaac
    60421 cacaacaagg aaaatccggc gggtgatcag catcccgcct ataattcccg gcgcgatgcg
    60481 atgcttcaga cattggaaaa tcagcgaaaa gccctggaag cgcaaattgc ttcatgtgac
    60541 caatactgcc gttggctttc cggcgccaat ccgtgtgggg cggatggcgg caagacctgg
    60601 gagagatgct tcaacagtgc gtcagatgcc aaacgcgcct gggaaaaaac ggcatggaag
    60661 ccattggaag atctggaaac tgataaaaaa aattacaatg ccaaacgaga actgtatgcc
    60721 ggcaatattt ttaatcagca tgaagctttg ctgaaagcaa tcggcaggct gaccaatgaa
    60781 gagagggcaa ggtttgccga tatggaaaag ctgaagaagg atgccctgga cactctggga
    60841 gcggtggatc aaagcgtccc gatcttttat ccggaaacgg aaacaaagca ctcccaggta
    60901 acggcggcgg acacgaaaca gtacctcaac agcttcagag gccagaaaac cgacggatat
    60961 ctgggcctgc cgaagatgaa gttcccgaag tggggtgagg atggaatggc tgtttacgag
    61021 tatgtccggc gcatcgagag aaacgtatcc aacgagagac agaattttga tcgattgagc
    61081 gaaaaacaca gtcaggcgat tcaatacatc gggaaactga aaacatacct caaagaaaag
    61141 gaccggtatg agcaggagat caaggaagca aaagaaagaa tctatgccct gcaactgcag
    61201 tctgatccca aggcgcaggt aatcccggac atcgaaaaaa gaattgccga aatgaaaaat
    61261 gtggaaaagc tggcgctcga aattgaagct gaattaaaca acaacttgat cgcggaaaat
    61321 cagaagcagc ttgccggcat agaggacgac ctgcaatatc tcgactatct gttcaagaga
    61381 atcgactatt ggcgcgatgc ggttcgggga aatacgctgg cgggatcggt caatgccact
    61441 gtggtggaag tagccgtcgc cacggaaaac agtgccggtt caaaccatca taaatacctt
    61501 gcgccctcat ggcaggactt gagtcttttt gacaaagcga aagccgccgc cgccgcaaat
    61561 gcgttgctga aggatgtcga gcgcaccggc atcgccaaat gggatcccaa cggggatacg
    61621 gggctcaagg tcatgctgga gaagaaggcc gcggagataa gaaattactg catcgacacg
    61681 cccgattacg ccatcgtgaa cgccaatgtg gtgtatgccg caaggattgc caggatggcc
    61741 gccgacgtga aagcaatccc cgcaagcact tacggaacca atacttataa agaaggcctc
    61801 aaaaaccgca cgttcgatga gaagctcggc gcagcgcttg gaaacggcac agcctttgcg
    61861 atgaatatag agatgaaaat gaattcgaac gaacagacgg tttcggtgac gcttgacaaa
    61921 gccaaagagc tggcggcgag ccacggaaac gccaccatca agacggcatc ctccgacatg
    61981 gtgaaggcga tagaagagca tttgtcgcgc tacaacgccc aggttgccca ggaggccaaa
    62041 caatgggaaa tcgataaagc caaagccgag gcggagcaga aactccagaa cgaatgtctc
    62101 gccaaggggg ggaattattc aaacggcgaa tgcaaaacca ttcctggagg cacgggatta
    62161 ggcacgggcg gttttccggg ccagtcggga caagccgcgg gtccgggtgc gattgcggcg
    62221 ggcgggccgg ccggaataaa tacaggagcg ccgcctgcgg gcaggggagc ggtaatgcct
    62281 cagccgccgg gatcgggcgc aaaagcgcct gccttccgcc cggaccaaac cggtcctgca
    62341 aaaggcgaac ctgtcggatt ttcagcctcc gctgcctcaa gaaatatggg cggcaggtcc
    62401 gggatggatg ataacctcaa ggataaagtt aaagatcttt atggccggtt taaagaagct
    62461 tatgaggcga aaaatgcctc gggcgtgatc tactgcctga gcgatcagtg ggaatccagt
    62521 gacggcggaa ccctttcgga tctccagcag aatctccggc ggacatttcg cacctttgat
    62581 caaattcagt ttagaatcga aaatatgcag atcagcaaca tcgcggagga ccggttccgt
    62641 gtcagctacg acgcagtcat caccagcaga atttacaaaa ggaacctcaa gcacgaagaa
    62701 aaatcgagcg ttcaggaaga agtgaccatc gggagttcgg gcaagcccaa aatatcaaaa
    62761 acgctggggg gaaggttctg gtatgtcaaa taaagccggt cccgcaaacc taaaaagcgc
    62821 ttgtggagca taaaaagtta tcatataaaa acaggcatga cagattgaag ccgcaggata
    62881 catcaaatga atttgagaag aatatctatt atgaaaggaa cggtaaaaaa atgaaaacct
    62941 cttcaatcac aggagtgtgc ttttcttccc aaagctttcg gtggctaacg gtgatcctca
    63001 tgtgtttctt ttttctgggg aatccttttc ttctgaaagc ggaaaacgaa tttcatgcgt
    63061 ccaatgaatt cagctacacg tataatgaca tcagcggtcc cggcagcagc cagtcatccc
    63121 tgcggcaggg tttcagctac ctgaatcttc tgaatatcaa cgatctcgga aactataaag
    63181 ggtttgatta caatttcaat ttcgggctca aactgacgga tgacccgacc aacgaccagc
    63241 aaaccgcttc catcaccaac cttcagggga gaatcaccaa caaggttcac accttgaacc
    63301 ttggcgacac cttcgagaca ttttcccagt acgcgctgag ttcggccgta aaaggggcct
    63361 cctaccggtt tgttaaggaa aactcttata ttccggaagt gagcctggtt tatggctatg
    63421 cctattcgcg atgggacaat gcctttgcga tttacgacgg cccggtgaaa gctgtcgccc
    63481 ggcaagtgat gggcgcgcgc ctcaaggaaa atctggggca gaatttctgg gcgggcttaa
    63541 gttatgttca atcgcaggat aatgaccgcg tcaatcccac ggattccctg tatctgaacc
    63601 gcgcctatac ttttgacatg gaatacaaac cgattcccgg cctgaccgtt tccggagaag
    63661 cctctctttc cgatcagaag gaatggacgg gaaatgacgg cagcgagacc acccgccacg
    63721 gcgcggccta cagattgacg gctgtgggag atgcggatcc cagccgggtt acgctggaat
    63781 acgaaagaat atcgcccaat ttcacaacgc tggtcggttc ggcaacgccg gacagagaaa
    63841 aggccaaggc aaaatggcgt tacaagtaca caaaaaatat ttcggccaat ttaggattcc
    63901 tctggtttag agacaacctg gacgggcaga aaacagcggg gcggacggac tattataaac
    63961 cggagatcgg ctttaccttg aaaaggtttc tgggaagggc atacggtaca gccgacatcg
    64021 cctacaaact ggatgtcaca tccaataata atacggaaaa ttataatcat atcgttacct
    64081 tgaactatca ggaccagttc ttcggatttc tggacagcga cacaaacctg ggctacacgc
    64141 tttatgacaa taaaatttcc gtcgaggaac ggaacaatga gttcacctac aatacctctt
    64201 tgagcagccg ccacactttg ggcatggttg tcctgaagcc tgccgtgtat ctgggcgggt
    64261 ggacgcagcg cagtgagctg gtggtcagca ccgaccagat ttatgagtat tccgcgggat
    64321 tcggtctgga tgttccggat tggaagctca cctccaccat caaggccggc cagaaccgtc
    64381 tggataaaag cgtcggccag gacacggaca agacgtttgc ctctttcaac ctcaattacc
    64441 gcccgccgtt tctcaaaaag ctcaatcagg gcatgctgtt tttcagggct aaatacaacg
    64501 atttcagtta cacgaacgcc ggaaacaact ttcgcgaaac cagcctgacc gcaggtttga
    64561 ttcttgagat ttaaaaagtt ttccaaagga ggaaaatcat gaaatacaaa agcaaaagtc
    64621 tgataatgat aatgtctttc gcgcttgtgc tgctttcttt tgcaggcgct caggcaaaat
    64681 ggtggatttt cggtgagtcg gatgaagagg tgtcgatcag ctaccttttt gtcaacaaat
    64741 catctttcga tgaaacaggc gcaaaaataa ccctgttcaa ggagatgctt ccggacggca
    64801 aaatcaccat cagcggaaag gccgtcgcgg gaaaagggaa aatcggcagc gcccgggtca
    64861 gcctggacgg gaaagaaaca tggaaggacg ccaatctttc ggaaggcggc gcttttgagt
    64921 acagcttctc gccggaaacg ggaaaaacct attctatctt tgtcgaagtt tccgacacgc
    64981 tgggcaagac caacgacgtt gcggctaccg ctcgtcaact gactctttcc gaacgcaata
    65041 tcatgagctt tatcagggaa gcgctcgaca aattgattgc cgcctatgaa aaccatgacg
    65101 caaaaacttt catgtcgctg gtcagcgagg atttcgccgg agacatgacc aatctggacc
    65161 gtgccatacg aaaagatttc agttcctttg acgatattga tttgaactat accctgaaca
    65221 acatcacgtc cgacagcaag ggcatctctg tttcttttag ctaccgcagg ttgctgatgt
    65281 cgaccaaaac cggacaatcc gtcaaagaca agggcgttac ggaattcgtc tttaaaatgg
    65341 gcgacaagag cgcaaggatt tacagcatga aaaatccgct gattttcggt atcaccggcg
    65401 cgccgaacgt ggcgacgggt accgttgcgt ccacaacaaa cgagcctgtc atcatcgccg
    65461 acggcagagg aaacgtggcc ttggtgccgg cgcagacttt taaccagatt gttatggatg
    65521 acagcgtcag gatcacaaac aacccggacg gaacttcaac cataaaaact tccgataaaa
    65581 cgataacggt ggacaagaac ggacagcaga ccagcggcgg cagtaatccc agcgaaacgg
    65641 tggaaaaggg cttaagaacc atcatttcgt taggccatcc tcccatgggg ttttcttttg
    65701 tcaatggtga ggttatgact gctgcgggag attttatgat tacgggcggc ggtccgggat
    65761 ttggttacgg atttgttgag cccagtgtaa cctttgtgga tcttggcgca aaaagtatcg
    65821 gcagcgtcaa tgaagctcct gcaacgggat atgcaaatcc cgccggcatg ggggtacggt
    65881 ttgaagaggg gcacacatac gcctttaaac tggccaacgg caaatatggc ctgatggaag
    65941 tcaaatccgt gacggaggaa tggattcccg gaaatccggg cccgccgccg cagccgcctg
    66001 cgatgagaat cacgatgaga tttgaataca aatatcagcc cagcggcgca cggcagtttc
    66061 tgccgtaaag ggattcttgc cgggtgacaa ccggttgttc caggaagaaa ggttgataaa
    66121 atgcgaagaa agattcagtt cttcgggatt aaacccgtat gtcttctgat cgtcatgacg
    66181 attgttttat gcttttccgg gcagtgcctt gcaaagggac ctgctcccaa aaaggcaaag
    66241 gctgcccagc ggcaggaagc tcccaaggct gctgttttgc cggctcccgc cgtgggtaaa
    66301 gcggagccgg aaaagaagcc ggcccccccc gcatcttttg gcgttgccgc accccgggac
    66361 gcggcggcct ttttcgagga agcccggaaa ctggccgcaa ccgatcctgt ttctgccgtt
    66421 gcgctttacc agcggggaat gatgctgaaa cccgatgcat ggaaagaaag aaaggagctg
    66481 gccgccctct acgaaaagca gggtcagtgg aatatggccc tggaggaata tgaagccgtt
    66541 ttcagggcgg acaagtctgc ggagagttat gccaaccggc tgcggatgct ggagaaggcc
    66601 ggttatttgc gaaccgcggc ggctgaggca aggaaagctt ttgctcaata tcccgaccgg
    66661 cctcagtttc ttctgcaggc cggtgagctt tttcacaaag caggagatga agcccccgca
    66721 acggccgctc tgcaggaata tctgaaattg aagccggatg acggccgggc gctgatgatg
    66781 cttggctccg tttatgaaaa atccaaaaag ccggccgacg ccctgaacgc ctatcttcag
    66841 gcggaaaaaa agatgaaagg caatcaggaa gcggcggatg cggcaaaaag gcttcgggcc
    66901 ggaaacgccg ttgcaggcgg gctgaccatt ttccttcccg aagggtggtc tgcggatcaa
    66961 gacggcatct tcaaccttca gagcggggag cgtgtgaccg tttccgtgaa atccgccgga
    67021 agccccgccg ctctggcgct gacatccgcg agggattcca tgccgaaaga gccgtttagt
    67081 gcggaaaatc tgaaacaaat ggaaaaatta aaaaaaatgc ggcaggaact ggctaagaaa
    67141 gatccggaat ccgcgaaaaa catgccgtcc ggcatgatgc ccattcacac ccagggagat
    67201 tttgcccagc tgaagggcgc aaaaaaagcc gttctttcca ccagtgagac gcttcagccg
    67261 ggaatggagt cggccgtgtc cgtcgccgtt ccctccggag gaaaaatata tgtctttttg
    67321 tggcgggcgt ccctgcccgc cgcggacgga gaaaagacgt tgagtttgct ccttggacaa
    67381 tccgtctggc cgctttaatc ggaggttctc atgatgtcac gattactcat ttccaatatt
    67441 tgccgctctc tgttgctgct gtttctctgc tgcttcaatc ccctggctgc tgatgcccag
    67501 gagtatcagc aggcggccaa cgctcttgaa aatcttgcct cggaaatccg catgaatgaa
    67561 gccgccgtgg gtcaggtgct catggaggcc atgcagtcca aacacgggga tatgcaaaaa
    67621 tggaacgatc tggacgcaaa gatggcggag ctgggccggt ccattgacgc caatctgcct
    67681 gaaatcagcc ggaatgcggc gattgtgctt gcgcagaatc ctgatcaata cgtgccgagc
    67741 atctccctgg ggcggacgtt tgaaggcaac cttcgaatgg cgacggacaa gaggaaacag
    67801 ctcggtgaac agtggaaggc cgaaaacgac aaactgatga gtataaacgc aaagattgcc
    67861 aaggttactg aacttatcgg caacgcgcag gcaggctctg ccatcgacgc tgtcatggtg
    67921 ctaaatcctt tgccggatga tttctgggag tttggaggag aggccacaat tgcggttctt
    67981 gccttatatt ccgggccggt cggctgggtt gcgcttgccg gcacagcagc ggctaaaggc
    68041 atcagtatgg tcaccaaatc atactacaat gcaaaaagtt tgaaagaacg cctgaaggtg
    68101 tacacggaac taagggacgt cctgcaggaa aagaaaaaaa tccatgaaga taatatgaaa
    68161 acgctccgcg acggcgagca ggaaatgcgt cagatcgaac agaccctgga aaagcacgaa
    68221 aagaaaatga ctgaatataa ggccagggtc aactccgcca tggaggcctt aaataatcaa
    68281 tacaagagcg cctttgatgc cagaaagcaa aagctggacg aagaagttcg gaaaatggcg
    68341 gacgcgccca agccggatct gaagccaaac ggatgggtct acggcatgga tcccattccg
    68401 cccatttctt ccggtgagta cagcggtgaa gtgaacgcga tgatttcgca gatgagatct
    68461 tacgcccagg cggtggaaga cggaggcgac ccggataatt tccagatcat ggtcacggac
    68521 tggcacaatc gtttgcacga tcggcacaag aacatcaaaa aagagcttga tcagaagaga
    68581 gaggcgcaca atcaggccac ccaggttttc tggaggtcgt atgacgcggc ctcggcggaa
    68641 tatgatcgcg cctggcgtat ctatgtcgat tgccgtgacg ggagtgaccg ctgctacgcc
    68701 tcgctgcggg cagcgggaga ccggtataat gccgctgtca gagccgccta tgcggcgctc
    68761 cggcccttcg gtcaggcgat gatcaatccc taccgtgagc tgataaaaat cgatcagatt
    68821 cattaccggg tccgggacgc tttctatcct ttccgggagc gagtggacaa tgccgcaaag
    68881 aaaaacaccc gggatttctg gaaccaatcc cgcctttggg agaccaaatt tgacgaaacc
    68941 aatgccaaag tttacgatgc cgtggcgcct gttccctact ggattgatca gtggaaaagc
    69001 cgggctgaaa gattggatga tgagattcag agcgctcttt actgggggcg aaatattgcg
    69061 gatgtccggg ccgagctgtt gtccacggca aaggatttga gggatttgca taaaaccgtt
    69121 caggaagcgg ccaaaaagta tgagagcgcc aaccaggagc gcatccagac ggccaacaag
    69181 gcgcaaagtg aattgaatgg tatcctcacc cgttatggac ggctgattaa ttattactgg
    69241 gcatccaact tttccataga ctggcttggt gatccaatgg aatttacgcc gcgtgctccg
    69301 gaccaggaaa aaaatatcac gtcgctggag gaatatgtta agaaaaaatt cactttttac
    69361 gaaccggaac atcttaaaaa atccctgaaa ttggatattc cggggattgc cgcacaatac
    69421 gagaacaaag cccgggaact gacgttttac acggactggg tcgatacgta tcgccatcgc
    69481 gcatcgacgg cagtcggcgc tttagacaga atcagcgtgg aaaagacggg tcagtacttt
    69541 tattccgacc ggggaggaac ggttccggag gttctgtcga aggaatttgg taaaccgccc
    69601 tggtcaacca ttgcccagga tgcggaaaag tatgtatcca agggagattt cgcgtcactt
    69661 ccctggggaa agtttcaatc ctgggagaac cttggaatat ggcagaaact ttacgccggg
    69721 caaacgctcc tgctgaataa gctgaacaag gatgccaaaa actacgttca ggcaaggtcc
    69781 aatggatggt ttatggcggt tcccgcacaa acgatggacc ccctgatcga aaactggaaa
    69841 aaactgcgcg aggttagcga acgcttcgac aagctggcca agcccgaaca tgacaaactt
    69901 gccggcggtc aggaacaggc gcaaaaggaa gcccagcctg ttttcgaaac ctggggcaag
    69961 atgcccaagt attcgcggga tatggtggcg gaggaataca ggcgtttttc caatgcatac
    70021 aactggctcc aagactatat cagaagcaaa ttcgatgcga ccaggaccac tcttcagccg
    70081 ccgaacaact ccgtcgccgt caatctggac aacctgattc tgggctatgc tccggcttat
    70141 gaaaaatgga aaaaacagca ggaagaagcc gaccggcgcg ctgaagaaca gaggcgccag
    70201 tgggaagcgg cggaaaaagc ccgcctcgag aaggaaaaga agcaggcgga agaagaggcg
    70261 caaagagccg ccagggcaat cgaagaagaa aagaagaggg cccaggaaaa tctttccctg
    70321 gtcaaggatt tttatgtccg cttcaaggaa gcctacgaag aaagaaatga ttcgcgggtg
    70381 atcagcatga tcagcgacca gtggcaggcg ggcgacggaa ccacgctttc cgatctgcag
    70441 atgacgctgc gccgcacgtt taaaacattc gacgagatcc gttacaacgt gcagaatctg
    70501 tcggtcactc ccctgcaagg agatcgttac agtgtttcgt atgacgtcac cattacgagc
    70561 cggatttaca aacgcaatct gaggcatgag gaaaaatcaa gcatcaacga agaagtgacc
    70621 attgaaaaat caggaaaagc aaagatttcg agaacgctgg gcggaagatt ctggtatgtg
    70681 caatgatttt ccagtaaaaa agttaatata aacagattgt tccgggatgt gctgatcatg
    70741 agcgtaagaa gagactggaa gttgatttat aagcggggga gattattcct gctggtgatt
    70801 ctggccacgc ttatgttggt gaatagtgca ggggcgttta gtaccgattt tgaagcagga
    70861 gttgattttt tcggtaactt cgtcgaaaaa ggcaccaaca gcggctatgg aacagtcctt
    70921 aaattagacc agcctaatct tgatcctgat aaatttaaga actttgattg ggcccggctg
    70981 accatggccc ggttttctac cgctctggac gttgtggaca agctcaatag gggggaatat
    71041 gacgatgcgc aacttgccct ggctctcaga gcaatccagg agatagccgg ctcggaaagc
    71101 gggggcaaat tgttaggcgc attgggactc agctcattaa cggtgaacgt cttcgtagtg
    71161 tcggtgaagg tctatgtagc ctctgtgagg gcggtgcggg aatcgacccg cgcggtgcaa
    71221 ctggaatcgc tctacggcca tttcgaatct attgccaata atccgcggct gagaaacccc
    71281 ggcagggtcc tgggcgaagg cgatccgatg cccgtgaccc gcgccagcat cgatctcctc
    71341 tggagagata ttgtggatga tgagaatgtg cgggcgcagt tctatgttta tgtgactcaa
    71401 ctgctgcaaa gagattggcc ggagggccct tactggctgg gctggtatgc ggctgcacat
    71461 cccccgcaaa aactaccttc tgcgccaaat acagtagtgc aaacaataac ggcaacgcca
    71521 ccgccgccgt ttttgcaagc ccaacctgtt gccgggccga caccggaatt acttgcatcg
    71581 aggagaaggg agatggaggg ctacattgcc agtctgctcg gagacctcaa ccagaaaagg
    71641 aaggtcgaag aacagttgac gcaggtccgt aagtccttta tcgatctgaa agaccggatc
    71701 ggaaggtccc gtgatctgga ggcgatcatg gtaaaattcg ctcaatctct ggagaggctg
    71761 ccggccgttg aagaatttgg aaaaaaatgt cccggctaca tccagaacgc ggagaacatc
    71821 aagagccagc ggtcgcttga agatctgatg gaacagagca agggctttgc ggaaaacgtg
    71881 atccagttca tcccggcaaa ggggttctat gccaaagacc gggcgaggct tctcggcctt
    71941 cttaaaacct gctacattca agccaaagaa aagattcccg ttgtggcaca gaaacagaag
    72001 gaggaaatgg aaaaaccgac tccgcggccg actgatgcaa gaaaggatga aacagtacaa
    72061 gacggatatg cctattattt caaggctttt gttgttccat acgcctggtc cgaggaacct
    72121 gacaccgcaa aacgcagcgt tttcgtcagc ctgagcaaag gaaagtggga tcaggccgat
    72181 gacgtatatg cccgatggac gggaaagact tattacggat atgatcgggg tcccggccag
    72241 acctacgtcg ttcactactc ggatgttctg acgaaagtaa ccgacctgta taataagccc
    72301 cgcaaccctc tggctaaaca ataccaggct gcctacgaca gctgtgacgc tgcctatcgg
    72361 gcgtctttcg ggcccagaga tgcctgttat gcggctgcaa aaaccgaaaa ggacaaacag
    72421 ccctgccagg atattgagag cagggcttct aatgcctaca gcttttgtca ggagagcagt
    72481 ggccgtccgc tccggttgca gattgaagta ctcgatgccg gcttcagccg cgcaaagcag
    72541 gccgttggct atcttctgga aggtgatgag agtctctatc gggagacgaa ggtggccatg
    72601 gacgaggctt tccggagcat tgtcgagctt cagcaggaga agaaaagaca gctggaaacc
    72661 ctgcgcaaag acctggccaa gtttgatgga cggcttcctc tggaggaaaa accccgggtg
    72721 gtggaagggc tggacagggc gattgctgat ttaaagaaaa acagttggcc gggagtgaca
    72781 aaagagtgga tgggcggagg gggaatatca tccagttcgg ttgtcatcga tgaacgcgcg
    72841 ctgagcctga gggaacgcgc gcgccccctt cccggaagcg tggatagctg ggaagcgaaa
    72901 atcatcgagg ctgaagaagg atggaatttt gccgttcgtg actggcaggc cttttatccg
    72961 accgttgatc tcaaaaaaca ggattatgac atgattacga tgtgggtaga caagaccttc
    73021 agccgggatg attttgatcg ctacgacagg gcggctaatc gtgttccggc gcttgtctat
    73081 tccgcgaaga cgaaactggg ggaataccgg actctggcgg gaacggaatt tcacaacatt
    73141 tccaccgatg ccgcctggct ttcctcgatc accgccgagt tcagtaaatg ggttgcccaa
    73201 aagcagaagg agggaattcc ctatttcagt tacagcccgg gattgcacct gttgtccagt
    73261 ccttatcctc actacctgac caagggtgaa gcggccttat acgccgcgga ggcgaagcag
    73321 ctcaagatct atgactttgt gcagaagtat atgccttcca gcctggccgc ttttgacgca
    73381 gaccttctcc tgggtaagat tcgtctggcc gaggaagata atttcattat tggctttaac
    73441 acaatcgccg gaaatatagc catttacaag agtcagatca gcagggccaa tgaccttttg
    73501 gcgagcctga agccggatat gggcgctgtt gcctatgggg aggtgctcaa agaggtcagc
    73561 acggttttgc ccgtgacgct ggagcgctcc aacgaggcgg aggataaagc ccgtgaaaaa
    73621 aacgccaaga gcatgaagat atcttatgtg ccgcttccct gggaaggccg cctgatcttt
    73681 gtggacgagt ctaaacttaa ctttcccctg ggtcttgctt atctggattt aaggaagcag
    73741 ctttccgtgg cgatccgaaa aggtcaggaa tacacgattg cggaacgggc gaaagcggcg
    73801 gaaaagaacg ggcagcagga acaggctaaa aaggagagcc cgaaaaccgc ggaagttgcg
    73861 ccgtcgccgg ctgtgtctgc cggcagagat aaggaagaaa tacaggcttt ttataatcga
    73921 ttcaagcagg cctatgagga gcgcaacgat tctcaggtat tgagcatgat cagcgatcag
    73981 tggcaggcga acgacggcgc aacgctgtcc gacctgcaga tgaacctccg caggactttt
    74041 aaaatgtttg atgaagtcaa atacaatatt cagaacctgt cgatgattct tgttgaatcc
    74101 ggaaacggcc gtttgcgcta tctggtttct tatgatgtca ccattaccag caggatgtac
    74161 aaacgcaatc tgaggcatga ggaaaaatca agcatcaacg aagaagtgac cattgaaaaa
    74221 tcaggaaaag caaagatttc gagaacgctg ggcggaagat tctggtatgt acagtaatga
    74281 tcaatacgga ataaaggagt cgtgtatgaa tattttcaag aggattttgc cggttgtctt
    74341 gttgctggtt atgacggttt ccggagcgga cgccatggcg caggaaacgt caaaaaactt
    74401 gtccgtcggc aagaaagcgt ccggaaagac gtcttttctg gagacgcaaa aggctgtgcg
    74461 ggaaacgctc gacggcatgg ttgaggccta caccgggaag aatgccaggc aattcatgtc
    74521 ttttgtcgcc gagaattata tgggggatga ttcgcttctg gagaggcgca tcagaaggga
    74581 tttcagcaaa tttctcgata tggacatccg ctacacattg aacaacgtga caaccgattc
    74641 ccgcaacgag aacatatccg tcgcggttag cttcacgaga agttacacgg acgtcaaaac
    74701 cacaaagcgg atcaataaaa acggttcggc tgcgtttatt ttccggatgg tggacggcag
    74761 gcccaggctg ttcaacatga aaagacccta catgttcggc gttgggaaat agtccgttat
    74821 ttgacgccca gcgcttcttg gagtttgccg cggttcttga tggcttcgcc gacgcgggaa
    74881 agcgcaaggg tgatgtcgtc gtccggtttc cccagcgccc tgtcggattg ccggtaaaga
    74941 gtcagggcgg tatccagatc cccgcggctt tccgcgcaga cgcccaggtt atacaaaagc
    75001 gcgtaagagc ctggggtaag gtttcgcgcc tgtccccaga gttcgcaggc gctgtccatg
    75061 cggcccttat ccgcgtattc caagccgcgc ttgagaaggt cttttgcctc atttgattcg
    75121 atgccgtccg tggaatcgat caggcggatt tcccgggtga catagaaagg agcaatatcg
    75181 cgccggaact gatttttgac ggaggctttg gcctgttcca aaaggacgga ttcgctttga
    75241 accggccgcg tgtcttcgca gccgttggaa gcggtcacgg cggacagatt gcgggagtat
    75301 acgattctgc cggtggccac atcgaccagc ttcggcgaaa cggcgaagtt ggcgacgcgt
    75361 ttcatgcagc ggacgttgta gttgcgccat tgaaggcagg cgccctgata gattcgcccc
    75421 ttttcatccc ttcgtttctc ataatggacg caggttgacc gtttttcgta gtagaagctg
    75481 tcgtcatgat tgttctgcgt gacgacgccc gtgtagattc cctgggcgcc gatcattttg
    75541 cccagcttta cggctgtttt cggatccacc aggccgctgt gtgaaaattt catttcgctg
    75601 acgattttgt cgacggaggc gcggtccacc agcgtgaaat acggtttatc gtcgatgccg
    75661 attcccgcga gcacgccttc gatttccgcg gcgaattccg gtccgccggg gccggtgaag
    75721 ggcaaaacgg cgacagcttt ggtcagcgat gcttcatgat actgcgcggg ctgcagcatg
    75781 ttgacgcgaa ttttcgtggc gcagccgcac agcatcagga gggtgaccag caaaattgcc
    75841 gaaaccagaa caggataatt accgtacctt ccgcgcgaat tgaacatcgc cgcctccttt
    75901 tattgcttca aatactccag cgtcagggag tagggcgaga aattgacgat tttaaagctt
    75961 cccttctgct ggatgctgtt ggccagatac agggtgtttt ccggatagct gaccacaaac
    76021 gaccccattt ccttttcttc cacccccgcg acccagacga tgttctgaag gatgcccttg
    76081 actttcagga ccgcgtccag atcctccacg cccgccacct gcacggcaat ctttttggtg
    76141 ttgccctgaa tgtattgccc gaccttgtct agcagggagg gggtcagggt ttccgccagt
    76201 tcctgcatgg cctgggccgc cgcgtcctcg acgctgttgg cgagccccct ggcctgttcc
    76261 acgcccgtgg aaagaatttc catgttgccg gtgttgttgt ttttggaaag aatccggtaa
    76321 ttgagacgga cggtgacgtg gttgaagggc atggagaggc cgtagccgat gtcttcgcct
    76381 tttttggtgg aaatggtgta gtccaccttc ccgatgatga tcagattcga caggaacttg
    76441 tacgtcaggc ttcgcatgga cagggttcgg ccggtgcgaa cggcgctttc gacgtctctg
    76501 gcgttcaccg cgtgcgtggg cgcgacgtcc accacgttga agccctgctc ggtcagtttg
    76561 ccgatgagcg tttcggaaag aatgttcttt tcttcaaatt catctccgcg ccgccccggt
    76621 ttccgcgcgg gaatgaaaag cgcgatggag ttgttcagac ccagctgcga aacggcgttc
    76681 cgggcctggg acggctcgac gcaggcgttg attttcacgt tcagaacgtc atcctttacg
    76741 gattcatcaa tcacctgata gcttctcacc acgccgcccg ccttggtttt catcacgtct
    76801 tcaacgaggg tgaagttttg cacgaggctg ccggccttta cttccgttcc ggcgacgtct
    76861 tcgatggccg tccacttggc gcgggccacg gcttccattt ttgccgaggg ccggtcgttg
    76921 ttgaggatca gcgcttcgcc gtccgcgtga acgcatttgg actgggccgg cgcgtcaggg
    76981 acggaaaaac agagcaggca aagcacgcaa aagaggaaag gtgatgtttt catgacgcac
    77041 ctctggaaaa taatttatag gttcggcaaa acggcgccgc gaaaatcttt gaatcgcgca
    77101 caaaactagc ataaagagga ctgcttttca atttatttgc cgatgcagaa accggagaaa
    77161 atccgggaaa gaacatcctc cgatattttt ttgccggtga tttcatccag cgcgttcagg
    77221 gcctcggaaa gctcaaaggc ggcaaattcc gaagactggc ccgttgcgag gcagttttgg
    77281 gccgccttga ggcaggcaag ggccttttcc agcgccagtt tgtggcgcag ccgggaaatc
    77341 atgccgcctt ccgcgggggc atccgtgccg ccggccaaat cgctcagggc ttttttcaga
    77401 tcctccacgc cgtcgccgaa ttttgccgaa agatcgagca ggcgcctgcc gtgaagcggg
    77461 tgaccgggaa gccggttgtc ccaggcgggc gcgaggtcgc gcttgttgac aaccgtcagg
    77521 atttggcagt cgcgtttttt gctttgctcc aggatccggt aatcttcgtc cgtcagcggt
    77581 tggctgccgt caaagagcag caggagaata tcggcctgct ccatctgctg ccgaaccagg
    77641 cttatgcctt ctttttcaat gtcgtctctg ggcggacgga tgccggcggt gtcggtcagg
    77701 tggatgctga tgccgtccag agtgatcgtt tccgtaatca ggtcccgcgt ggtgccggga
    77761 atgtccgtga cgatggcttt ttttttgccg gccagccggt tgagcaggct ggacttgccc
    77821 gtgttgggct tgccgacgat gaccacgcgg atgccctgcg tgatgagccg ggcctgtcgg
    77881 taggtggaca ggagcagctc cacgccggcg gcggcctgga gaatttgccc ggggacggcg
    77941 gggatttctt tgtcggaaac atcctcggta aaatcgattg cggcttccag attggcgaga
    78001 ccgtcgatca agagtgagcg cagttcgtca attgagttcc ccagcgatcc ttttagctgc
    78061 gacagctcaa gctgcagcgc ctttgccgac tgcgcgcaaa tcatggccgc cagcgcttcc
    78121 gcctgcgaca aatccattcg gccgttcaga taggcgcgtt cggaaaattc accggggcgg
    78181 gccgggcggc agcccagcgc cagaaactgt tccataatgt tttgcagaat caaagggttt
    78241 ccgtggcagg aaatttccag aacgtcttcg ccggtgaaag aacgcggttt tcgcatcagc
    78301 gtggccagca cttcgtccag aaccgttttg ccgtcagccg cgatgatgtc tccgtgatag
    78361 caatggtggc tttgccaggt gcagttggaa tgggcgggac ggaaaagccg ctgcgccagt
    78421 tcaggggcca gagggccgct gacccggatg aggcccacgc ctgccgtgcc cgaaggcgtg
    78481 gcaatggctg cgatcgtttc tgtctttgtc accattcact attcaccatc caccatccac
    78541 cattcattaa aagctatcgc cggccggctc tttccggctt ctgtttttac ccgggcggag
    78601 gctgtttcct tttttggccg gcagaacgat gaccttccgg tactcgcctt cgccgcggct
    78661 tttggtgatg agctcttcgt cttcctgcag cgccagatga atgatccgcc ggtcgtgagc
    78721 gttcatgtgc gacagcgaga gaggcttctg ggtttttttc accttctccc gaatccgctc
    78781 ggccatcgcc agcagggatt cctctcttct tttgcggtac tcttcggaat cgaccacgat
    78841 cattttgcgc tgtgcttccg atttatggac ggacttgttc aggaggtact gcagcgcgtc
    78901 cagattctgc ccccggcggc cgatcagaag ccctccgtca tccccctcga tgctgaggat
    78961 gatcgtttca tccgtttcgg cggcggtcac ctggcattca aacgtcattc tgccgaggat
    79021 tccggccagg aggtctctgg ccttaagcgc cgccggcgtt gtggcgggaa ccgcttcaac
    79081 ccgcggcacg gcgggcgggg gggattgttc gggcgccgcg ggcggctggg ccggtttcac
    79141 ccgggccgga cggggagcgg atgccctgga ctccgcgggt gcggacctcg cggcccggcg
    79201 ctccggttgg gcttcgtgcc gtttttcctg ccggtgttct tgcctcgatt ccggttttct
    79261 ttccgcaggg cggtcggttt tttcttccgg tttgccgtcg gattttgctt ggggccgttc
    79321 cttctgccgg gcggagtcct ccgtggagaa gtccatatcg aaggaaaaga tcgaggcgcg
    79381 gattttggcc ttcttcgcgc cgattcccag aaagccgttg gacccttcgg aaatgatttc
    79441 gatgttcagt ttttcgcgcc cgacgccgaa ttcccggcag gcccgttcga tggctgcgtc
    79501 aatgtttttt tcttctatgt ccagtatctt tgcgctcatt ttgaactccg tcgatcctat
    79561 ccggtcgcca gctttttatt gatgtagtat tgctgtccga tttgcaaaat attgttccac
    79621 agccagtaga tcaccagtcc cgacgggaaa ttcaaaaaga agaaggtaaa gatcaccggc
    79681 atgaacagca tgattttctg ctgcgtcgga tcgcccatgg cgggcgtcat tttctgagtg
    79741 atgaactgcg tggcgcccat gatgatcggg gtgatgtagt aggggtcctt tgccgacaaa
    79801 tcctgaatcc agaagaagaa gggcgaatgg cgcagctcga tggaatacat cagcgccttg
    79861 tacagtccga agaacacggg gatctggatc aggatgggca ggcatccgga aaagggattg
    79921 accttgtgct cccggtaaag cgccatcgtt tcctggccga ttttggtctg atcgtttttg
    79981 tatttttgct tcagggcctc gattttcggc tggagctttt tcatctcgtt catggacttg
    80041 tagctcatgt tgcccagcgg ccagaatacg attttgatga tcgtggtcag gatgatgatg
    80101 gcaatgccgt agttgaggac gaatccgtag aggaagttga ggatgtaaag ggccgggatg
    80161 gccagccatt tgaaccaacc gaaatcaatg gcgttttcaa gaccgacgcc caggttcttg
    80221 agcaggtcgt attgtttggg gccgaggtac agagagtagg aaaaaacgct gttctgaccg
    80281 ccgggaatga tttccttgcg gcccttcatg ccgacgccga ccatgttggc cgcgtcgcgc
    80341 gtcaggacgg cgttggtcag agacgggttt tccggaatga acgacgcgat gaaatacttg
    80401 ctttcaaacg ctccccacaa cacgttcgga ccctgtacgg actggctctg gagctttttg
    80461 acttcctcca gctcgatgct gccgccgacc gagacgatgg ggccttcatg tccgtagctg
    80521 tcgtcgatct tctgaggatc gacatactgg taccagttca actgcggaat ctgggtgatg
    80581 ggcgctccgg tgagattgtg aaccttgacg tccaggccca gggtgtatcc ggacggatca
    80641 aacgaataga tcttttcgat tttgacgccg ccctgggatc tggaaaaaac cagttgctgt
    80701 ttctgctgag tgccggtcaa atccatgcgg tttccggccg cgtcaaaaac ttcgtccggt
    80761 gcgacatcga cgccggattc ggggaaggtc accgccagcg gatagggcat gccttcgcga
    80821 acatcgacca gctccacaaa ctccggcgtt ttgggcttga ggggggcttt tgtaccggtc
    80881 accagccctt tgattttcgg ccagatatca tcggtgcatt catggcaatc ccgatagtag
    80941 tctttgagct tcagggattt cagagccgcg ccgcgtgtgg agaaaacagc cgtataatga
    81001 gcggtttcca ccgtgatgtc gcggggaggc gcctgtggcc caactgccga tttcagcgcg
    81061 ggccgggccg aagccgtggt cgccgccggt gcgggagtcg aaggagcgtc cgcatcctgc
    81121 cgaacctgcc cggtggatgc ctgcggcaca gtgccttttg acggcacggg gggcttgaca
    81181 aaaaagattt gatacaccaa aagaacggcc agtgaaagga cgatggccag aatagtcctt
    81241 ttgtccatga acatacctcc caatttcttg tttactggac gggatcgtat ccgccgggat
    81301 gcaggggatg gcatcgcaga attcttcttg cccccagcca gacgcctttg gccgggccgt
    81361 gcagccggat cgcgtcaatc gcgtattgcg aacaggacgg ggtaaatcgg cagcaggacg
    81421 caaaaaagaa tggagaaata caggcttggt acagacgaat gagaaagaca aagaaattga
    81481 ccaaggtttt cctgaacatg ctttatttgc cggctttccg caagaagagt tcggtcaatt
    81541 ccctggctgt ttcctgatag ttggaaggcg gtgtattttt tttgaccatg acgacgacgt
    81601 catgccccgc tggaaacaat tccttgttgc ggcggaagaa ttcccgaaga agccttttca
    81661 ttcgattgcg aaaaacggcg ttgcccgttt tcttcgtgac ggtgattccc aggcgtttgg
    81721 cgcccttcgg gttgccgcag gttatgatgg taaagaatcg cgaacttctt ctgaccccct
    81781 gttcgtaaat ggtccgataa cggctcttgc tggttaccct ttccgctctt cgaaaagatt
    81841 gcgctttcat tgcggaagaa acccaacatc ccttcgaggc gttccatatc ggaattaaac
    81901 ggttaatttc tttctgcctt tggctcttct gcggctcagc acctgcctgc cggttttggt
    81961 cgccatgcgg accagaaatc catgggtcct ttttctcttg gtattggatt tattcgtcaa
    82021 agtctttttc atgttaaacc cctaaaagta atagggcatt gcctaaacca cagtcacctt
    82081 catttgtcaa ggtcaaagtg gtcctgttca cgcagctttt ccgccggttg gccgattgac
    82141 ggggcgctgg tccgcattcg gaagtcgttt ttgagttttt ccgcagatgc cggttcatct
    82201 gcggcgggtc caattccatc caatcggctt tttaaagaag attccattcg gcatcggaca
    82261 gccagcaacg ctcgcccgcc ggattcgaga tcgatgatga ctttttttct caagtgtgct
    82321 agggagccct cacacaaaat atcgtattgc ggcaaaaaaa gacagtcttc gcgtccattg
    82381 aaatttcagg cgctgcgggc taaaaatatc aaaaaaggag tgtaatcatg gcattaaatc
    82441 cgttactcga ttccagagat cagaggtttg tcctgtttga agcgctggag gcggacaaac
    82501 tcaaccggta tgagaggttt gctgatttcg accgcgacac gtatgaggcg acactggacc
    82561 ttgccgagca gattgccgtc gaacaggtct atcccgcgaa cgcgcaggcc gacaaaacag
    82621 gcgcgatata cgacccggcc gccaaaaccg tcaaagttcc cgaaggtttt catgcggcca
    82681 tgaaaagcta cagggaggcg ggattttcgg gtctgagcaa ctctcccgaa ttcggcggca
    82741 cgggcatgcc tgaaaccgtc tatcgcgctg cgatggagta tttctgcgcg gccggcgtgg
    82801 ccttcagctc gtatgatacg ctgaaggtcg gcgcctgcaa cctgatcatt catcacggtt
    82861 ccgaggaact caagaaaatc tatgtagaga aaatggttgc aggccagtgg ggcggaacca
    82921 tgtgccttac cgaaccttcc gccggctccg atgtcggtgc gctgaaaacc cgcgccgtcc
    82981 ggcaggccga cggcacttac aaaatcaccg gccagaaaat attcatcacg gcgggcgaca
    83041 atgatctgta cgaaaacatc attcatccag ttctggcgag aatcgacggc gatccccccg
    83101 gaacccccgg catctccatt ttcatcgttc ccaaatttca cgtcaatccc gatggcacgt
    83161 caggcaagcg caatgacgtc gcgaccaccg gcatcgaaca caaaatgggc atcaaggggt
    83221 cggccacctg ttctctctct ttcggcgaca acgaccagtg cgtcggctgg cttctgggtc
    83281 aggagcgtca gggcatgaag atcatgttcc agatgatgaa cgaagcgcgt ctcgacgttt
    83341 cacagcaggg tctgggcacg gccagctccg cctatatgca tgcggtgacg tatgcgaaaa
    83401 accgtctcca ggggcaggac ccggccaaaa agggtgattt tacctccgtg ccgatcatca
    83461 ggcatcccga tgtgaaaaga atgctcatct ggatgaaggc acaagtggaa gccatgagaa
    83521 tgctgacgct gcttgccgcc tactcggcgg acatggcgaa tgtcgaaaaa ggaacaccgg
    83581 aaggccgcga ggcgcagggg cttttggatt tcctgattcc cctgtgcaag gccggcaaca
    83641 cggacctggc ctggcttgtc acttccgagg ccatgcaggt cttcggcggc tacggctact
    83701 gctcggagta tccgatcgaa cagctcgccc gcgactgcaa gattctggcg atttatgaag
    83761 gaaccaacgg cattcagtcg atggatttga cgatgcgcaa gctgctcatg aatccggaca
    83821 tgttcaattt caaggttttc aaaaagagaa tcgccctgag ctgcgaaaag gccgggggca
    83881 ttgtggaaga caaatatatc gcggccatga aaaacgccgt tgacgcgatg gacgcaaccg
    83941 tggcgaaact gacagaatgg agagaccgga aaaggattct cgatattctg gccgtcgccc
    84001 agccgctcca gcaggccatg cgcatggttg cgcacgcctg gatgcatttg tggtccatga
    84061 cggttgccat cccgaaactg aaagccatcg ctggcgacat caagggagaa aaagcggcat
    84121 cgctggcaaa ggacaacgcg gaagccgcct tctacatggg caaagtcctg tccggtcaat
    84181 tctatctggg tgccgagctg aaacatctca ccggctatct ggattatgta acagccggcg
    84241 agcccgcggc cgtcaacgaa tgttttgaaa acatctttac cggggcgttg ccccagtagg
    84301 aaggccgccg ccgcacgctc cctgatgcct gctttgaaaa gccccccccg acgctttcac
    84361 gcggggggat tttctcaaag gatccgccgg agggacaatc cataagtaca tggaaagacg
    84421 agtttaatca gattttggaa aataatcggg aaataatgca atttcttctt gacagttttg
    84481 aacatagaga gtagaagttc acttctgtcc gtcagagatg tgcttgaagg ccctagcagt
    84541 tttcaagagg tttaccgatg gtgccactct ttgttgtcgg atgattcctg tttgcggacg
    84601 gaacaaaatt tgaaatttca gcttgacaat gaaatcgaat tgatttaaat gtcacagctc
    84661 gtttcaagaa ggctctttgg aaattgaata gtgggaaaag aaatttgtgg gtcctagtag
    84721 ttttttgaag tatcaagcga gttcaaaagg aagatttatc ttcctttaca ggattttaaa
    84781 ctggagagtt tgatcctggc tcagaactaa cgctggcggc gtgcctaaca ca
//
LOCUS       DAOPAF010000002        80221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_347_length_80221_cov_53.454120, whole genome shotgun sequence.
ACCESSION   DAOPAF010000002 DAOPAF010000000
VERSION     DAOPAF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 80221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80221
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_347_length_80221_cov_53.454120"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLQ77_00285"
     CDS             complement(<1..358)
                     /locus_tag="PLQ77_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09147.1"
                     /translation="MLKDHRPYYVKRFYNRFQKMYAQYFLAPQFEYFGRGLVFMKPWH
                     VEIFGGPVSLGDHSNVIATPDKKVRLTVWPAWKSAGKIEIGRCCLICPGTRIMAATSV
                     TMGDGCMTAQHVSISDS"
     gene            complement(453..1478)
                     /locus_tag="PLQ77_00290"
     CDS             complement(453..1478)
                     /locus_tag="PLQ77_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09148.1"
                     /translation="MELNISLSEELKPIDVEKVAEFIASLQKENGEIPWSRGGKTDPW
                     DHIESAMGLSVAGWTAEAERAYAWLVSAQLPDGSWWSEIRDGVVTNSTKETHFAAYIA
                     VGVYHHYLITGDRTFLRTMWPSLSRGIQYAVNMQAPDGEIYWARNREGAIDRMALLTG
                     CSSIYMSLKCALAVADLLDIKKPAWQKARERLGETIRSRPDLFNMIKSRFSMDWYYPI
                     LCGAVRGNEAKARIDHLWDKFVVPDWGVRCVSDRPWVTMAETSEFILTLAAMEDHTRA
                     RAIFSWLGDKRYFDGSYWMGVTFPDTVIWPEEKTGWTAAAVLLAWDALNGMTPASKIF
                     AHSSWPE"
     gene            complement(1483..2184)
                     /locus_tag="PLQ77_00295"
     CDS             complement(1483..2184)
                     /locus_tag="PLQ77_00295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09149.1"
                     /translation="MITIDLQELNLKPGFAVLDAGCGTGRHLRALARLPGLKIFGIDR
                     NPADVAEAAKALSEMPDALSREVSVEAADITKMPFANETFDCVICSEVLEHIPEHEAA
                     LKELVRALKPRGTLVVSVPRYFSERICWMISPAYSSDEGGHIRIYKKKNLQKMLAVQG
                     MKCWKINYKHALHAPYWWLKCLVGIKNDNNYFVKLYHRFLVWDMFCKPRAVRVLEEAL
                     NPVIGKSIVFYSKKG"
     gene            complement(2171..3424)
                     /locus_tag="PLQ77_00300"
     CDS             complement(2171..3424)
                     /locus_tag="PLQ77_00300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL09150.1"
                     /translation="MKKNSNSGPLKICLLTYRGNPTCGGQGVYIKHLSKALADEGHFV
                     DVISGPPYPQLDDRVRLIKMPSLDLYNPDHLFRPERFGDLARPLNLYEFASVCAGGFP
                     EPFSFGERVYAYLKKHRGDYDIVHDNQCLSYGIGRLAQKVMPTIPTIHHPITVDRQEE
                     YKAARTLRQKFRVFRWYSFINMQLKVAKSFSHIITVSAFTRKDIAKEFSLDENKFRVV
                     HNGVNNEYFYPKQNGPRPENSLIVTNSADTPLKGLRYLLEAVDEIRRKRPIRLTVIGQ
                     PKKDGIIENLVARLNLSGTVHFTGRIANEEFADYYAKSTIAVVPSLYEGFGIPAAEAM
                     ACGVPLISAAGGALPEVVGDAGIIVPPADAPALARAIEHLLDSSEERQKYAREGLRRV
                     HSVFSWEKAARESVEVYREAIHDYH"
     gene            complement(3421..4071)
                     /locus_tag="PLQ77_00305"
     CDS             complement(3421..4071)
                     /locus_tag="PLQ77_00305"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPL09151.1"
                     /translation="MIDLHTHTLFSDGALIPAETARRAFVAGYKAIALTDHVDDSNID
                     FVIPRMVRVCGKLSEKGDIFVIPGVELTHVDPSDIASLAAEARKLGAKIVVVHGETLT
                     EPVPPGTNLAAIRAGVDILAHPGLITREETKLAAKKGVYLEITTRRGHSYTNGHVAAM
                     AGKCGADLVLNNDAHAPGDFAGRPMAVNIARGAGLSEKEIVIMFKNSQKIVQRARA"
     gene            4302..6110
                     /locus_tag="PLQ77_00310"
     CDS             4302..6110
                     /locus_tag="PLQ77_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPL09152.1"
                     /translation="MHKFTLDKPDNLIDWWQESEAKFSDRRLFGTKNKSGVYEWVTFG
                     DVGTRIRNLRAGLAKLDINRGDVVGIIANNRVEWPIVAFAAWGRIARFVPMYEAELVQ
                     IWKYIIADSRIKVLFVSKPEIYEKIKDFPKDISTLKHIFVIDAEGDNSMAALEKKGAA
                     KPVAAQVPKPDDIAELIYTSGTTGNPKGVLLSHGNFTSNSLAGLKMYPELAENEGRDV
                     VTLAILPWAHSFGQTGELYAVIRLGGSIGLAESPATIVDDIAKVKPTFLVAVPRVFNR
                     IYDGLWAKMNKEGGLAKKLFVMGVEAGKKKRELAARGQSDFLTELKFRIADKIVFSKI
                     REKLGGRVKGAMTGSAAMNPEIGHFFFDIGLPVYDCYGLTETSPAATMNASYAYRLGS
                     VGRAIEKVRIVIDSSVVEEGATDGEIIVYGPNVMKGYHNRPEDTAAAMTPDGGFRTGD
                     RGRLDKDGYLFITGRIKEQYKLENGKFCFPVSIEEEICLLPLVQQAVIYGYNRPFNIC
                     IVVPDFDVLKNTAKEKGLPTDINELVERQDIVDMYGAAITAHLKNKFGGYEIPKKFLL
                     IPEPFTLENGTLTQTMKLKRKVVLDKFQDRIEALYR"
     gene            complement(6196..6465)
                     /locus_tag="PLQ77_00315"
     CDS             complement(6196..6465)
                     /locus_tag="PLQ77_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL09153.1"
                     /translation="MNRAELVEEVAKVTSTKKEADAAISAALAAIQKALKKGDSVTLV
                     GFGTFSVSKRKARKGRNPQTGEAIKIAAKKVPVFKAGKGLKDAVK"
     gene            6822..8654
                     /locus_tag="PLQ77_00320"
     CDS             6822..8654
                     /locus_tag="PLQ77_00320"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HPL09154.1"
                     /translation="MSAQPVKIPTTLSASEFFLKIVEIAPRLPSMIFNLIKGLKSIGG
                     KTNNSWGLELEKIAARFPDNPAVKSPDGTLTYRELNERANRFAHYLISKGVSRGDVVT
                     LCFENRPELLAAYCGCSKVGAIASLINTNQRGDSLVHSFNLNRGKVVLIGEECCEFFI
                     EARSGIDLSQSTLGMVRDAGQFKTKDVPDITDELARQNGANPSTTKSVTLKDTMAYVY
                     TSGTTGGLPKAAVIINKRTLSAMFWFGKFVMPVKPTDTIYIPLPFFHTNAITVGWPPA
                     LYNGAAVALRRKFSASKFLDDVRRFQATHFIYVGEVCRYLMATPAKPDDHQTTLKYIC
                     GNGLRPDIWMPFKKRFGIDKIYEFYGAAEGVGVFTNFLNIDYSVGTSVTPFAIVAYDT
                     ENDRPVKDEKGFLKKVGKGETGLLIMEISEKTPFAGYSDKKKTEEKIFRDVFRKGDLW
                     FNTGDMLRDMGFRHAQFADRLGDTFRWKGENVATQEVEKAIDTFPGVKQSAVYGISMP
                     GSDGKAGMAAVIRDENEPFDLARLAAHLKASLPKYAVPIFIRFVPDFEWTATHKIKKT
                     NLKNEGYDPAQVREELYALLPGSETYVPVSDEIYRNVTAGTYKF"
     gene            complement(8651..10444)
                     /locus_tag="PLQ77_00325"
     CDS             complement(8651..10444)
                     /locus_tag="PLQ77_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPL09155.1"
                     /translation="MKKAWVLFLCWLILLGSPAVVQDAKAQDSSPLKVVLIFDMGGRG
                     DGGFNDSAYSGMEKAVAELGVKAVYVEHKRNLELDYAVSGAAASDAEMIIGVGFAFSE
                     KFDHLAGRHPDKKFVVVDYSANYDERGNLISPPPNLAGLTFREEEGSFLVGAIAALKS
                     RSGKIGFIGGMDSPIIRKFQAGYEAGAKAVRPGIRIYSRYAGITGQAFRDPRKGYRIA
                     VSMYKNGADVIYHAAGETGAGLFQAARKMNRLAIGADIDQSAQAPGRVLTSMLKNIDG
                     AVFEAVQSCAHGNFSGGLKTLGLKERGVGFVYNNQNKKLIPESIHQQVQALRAKIVSG
                     ELAVPEASGHRTALSRQELRDLLSRLQSEIQTTLNRLDNDLRHGAKVLAGKDLRGDFA
                     RGILQGLYRANPYIIDCETVSDSGIMLAVEPPAHRSSEGADISRQSHMVRLFKTHTPV
                     LSGSFQSVEGPRAVVIHHPVFLKDRKFSGSVSALFAPEYLLSSMIGPIAAYMPVSIFL
                     MQTDGLFIYDVDAKQIGLNIFTDPLYQPFPELIALCRRMADTREGSGVYRFYRKVGEA
                     PIFKTVEWRTVELHGTPWRLAAAFAGDGVER"
     gene            complement(10483..11343)
                     /locus_tag="PLQ77_00330"
     CDS             complement(10483..11343)
                     /locus_tag="PLQ77_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPL09156.1"
                     /translation="MRKILFLAAMIILLAAPVYAAQSTIKIAEGSACMGDDKSRKQTE
                     TAALADAKRNAVESVKTYVSSATEVKDFELQKDLVSAYANATVEVLETLEKGWYKDPG
                     QGDCYRIKLKAEVIPDEKAMKKAGAKAADAALDDPSLPLTVKVWTDKTSYAAGEKIRI
                     YLKGNKPFFARVIYRDAVKNNLQLLPNPYRRDNYFQGGVIYEIPSGKDQFELEVNPPF
                     GEENIIVYASVSELGDLKLKDEGSIFAVQTRSKDIGVKTRSVKIKAASDDAGLAAEFS
                     EVKAVVKTRK"
     gene            complement(11362..14616)
                     /locus_tag="PLQ77_00335"
     CDS             complement(11362..14616)
                     /locus_tag="PLQ77_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPL09157.1"
                     /translation="MVFTTEAFCAKPLVPAGSSQGPDIFVQKGHSTNVNTIDFSPDGK
                     YLVSGSSDKNIKLWDVETGREIRTFVARDMVDFVAFSPDGQSLLSLESKGGAALWNVK
                     TGREIRRLGKKGKPLLAIAAAFSPDGTSLAVVGGQDEKVPGGDLTFRNIQLLDLNTGR
                     ELMIFKGHSSSIHTIAFSPDGKYLASGSETPNLNKGKKDDTVRLWEVKSGKEIHRFAG
                     HTEGVSKVAFSPDGQFVASIGEDRNIFFWNVGSGKQAKALSGFPAGSVKTNGTIAFSP
                     DGRRFVAVSSFPPVMILWDMVTGQVEAGMTDDHKLDDAAVRFSPDGRLIAVSATDKVS
                     LWDAAKHRKIRSFGGDVQGINLAFFSADQEQVKIAGNEELAVFDRRQGRLTGRRTIEP
                     FDNWWAKGQNEWGNRRFFKIIDKGYQVIDSRTDRAIISDAADRYPTAYDSPQQAVTFS
                     PDGRYALLAEKDGGKVSLLDVRSRRSIELVNFPDHVSLSSSDGYAAFSPDGSLVAAAS
                     YSHDEERGVLVIWNTATGKQSIRLRANQKREGFSQVAFTADSKQIVAAEGGNQGILGL
                     WDLTSGREIRSFPGHPGFITSLALSGDGKFVVSGDWIGTVKLWNLRQGGEVKTFKGHT
                     DEIKSLAFSRDGNYILSASKDGTARQWDIASGKEIAQFVSFTNGEWIVITPEGYFNAS
                     PGGAKHLNVRIGLNVYSIDNLFEKFFNPVYVASVLQGKKVEATADIRKGILSPPEVRI
                     TSPAAGSVLNTDTLAVTVAARDTGGGIDEIRLYHNGKVVGGDQRAVKIVPRGGETVRQ
                     YSVSLVDGANTFRAVGFSRDRTESNPYELMVRLKAPSKEVSLYVLAVGINKYKNPALN
                     LNYAEPDAKSIAGFFSQRGGGLFRKVNIREIYSEQATRENIVSQLNELQNTNPQDAVL
                     IYLAGHGESLNDKWYFIPHELTYPEREADVRTKGVSSDDLSGYIKNIKAQKILLLVDA
                     CKSGAVLIAFRGFEDRKALSQLSRSTGVHVVAASTKDQFAAEVKDLGHGVFTYTLLAG
                     LGGKAAGPGEPVTVRKLMVYVEEQLPEITKKYKQEAQYPVVDSRGMDFPLVLGR"
     gene            complement(14783..18982)
                     /locus_tag="PLQ77_00340"
     CDS             complement(14783..18982)
                     /locus_tag="PLQ77_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPL09158.1"
                     /translation="MVSGRRFSRLSTIWFCFLSLWILFPVVVSAGDSPPSPCGTEKKA
                     EIFIPAGHSGSVNAVEWSPDGRFILSGSSDGSIKLWDAQSGRLIRTFPDGEGILYVHF
                     LPGGKSFFSVNEKGGVGIWNIDGGARTGGFSLSGEKEPMIIDPVSFDGSVVRAIVSSR
                     LYAAQLSEGKVLPAEQLPEGAPVKKYFSGYDFGHKNLVTADGKRMISAVPEDPSDTRF
                     KKLILWELSTGNILFTLDGHTDRIDAVALSLDESYAASGGRDKTIRVWDLKTGKAAAV
                     FAGHQKEIESLAFSPDRKYVLSGSRDHTLRLWDIEAGKEVRAFAHRARVTLARFSPDG
                     RFAVSGDADGDIRIWDARTGEEVRALKSQADSGYVLVMSPDGRHVLSGTPDGKIRLWD
                     KTSGKLLETIQAHTERVAAVLFTPDGKYALSAGEDKTLKLWNPKGGTLLRTFAGHREK
                     IVRAAISPDGRRILSLDGSSEVRLWDLDSGAVQKTFPFPGRALSIGFADDSRHLLIAY
                     DDKVLDHRMIKVLTPEGREVKKYMSADFHFSEDGKYFLANEYWELTERGPQAFAPSAG
                     PKKQKLYLDSAKKSTLVDIAGGGILGRFGQSGAFLSLSACAEEGIVLTKSRYDRNIVL
                     WDIRTGLEIRRFAAGHGLLTYDGRKIVAPSGNTLQAFDRASGAPLAPFTGRASMEISA
                     LALSPDGSVAVTGDVAGEVQLWDIGAGTLKKSLPAQEKEMILATAVSFDRRFAASLAR
                     SGVIRIWNMNDGRKVSEVKTEYKPIYAEELAAGDFVDYGSGILAFSPDGLRLACGSGV
                     WETATGKSLLAFAQPNVPYPWVAFSPDGRFLLSRNMIWDASSGQLERVLECGKNAAKS
                     YYSLNGRLILCADLEGGFYTIDAATGRLTRKFADFVPPGPFDVSRDKKMMVAVDKDQN
                     EMTVWDLTSGRKNASIGVEQEVLSVSLTSDRRRAILRHGISISEYDLPAGKEIVQLVS
                     FTDGGWIVMTPEGYYNASSRGDRHINVRVGDSVYGIENYRESFYRPEVVKRALAGDSL
                     KDLPKLTDEKEPPKVRIVDTPASVAADEVHVRLNLDEQGGGIGDIRLYLNGTAVVLDS
                     RSVSEPEKSQRAVFRAYPLKLARGKNVIHAAALNGDNTMRSNKAAWEVVTASYAAAAG
                     PSLAVLAIGVGEYKDPGLKLPFAAASAELVAGALKGASEGIYEKPAVKTMTRPEETTM
                     EAILREIQTHQTLRAEDLFVLYIAAYAVVEDGEYFLLTSNVNSLSAEKLKTDAISLRK
                     LMEALGNIPTAGKLILIDACNPRIQSADAQAAFQTRAMSEETALKIFGGTLGLTVLAS
                     SAPLSEAVAGYQGHSLFVHLVAEGLRGAADRDKTGSIRSTDLTAYVRVEMPAQSEKIF
                     KHTRYPVVSISGQAFSIGKVK"
     gene            complement(19028..19525)
                     /locus_tag="PLQ77_00345"
     CDS             complement(19028..19525)
                     /locus_tag="PLQ77_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09159.1"
                     /translation="MPSREIATTVFGRNALTAGCICHAAALLAGMTTSSGADVFFKMI
                     YPASWVLMTAGAYAEMRRRGGRPLNSWRFYAAATASVFPLLGPPLVLGLIYTFAQIGR
                     EGQGDLSGFMPALGRMRGNLLTVFALILLLFLFFAFIHSRHDPYFQKNRQHGKIQQLC
                     GAGIV"
     gene            complement(19596..20150)
                     /locus_tag="PLQ77_00350"
     CDS             complement(19596..20150)
                     /locus_tag="PLQ77_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPL09160.1"
                     /translation="MSSREGKIRTANGSLWYGVRGGGGNRMPVLVLHGGPGFSSMDNG
                     LEPLWKDRPVFFYDQLGCGKSDRAPDKNFYSVENYVAELAEVRAALKLDDVCLMGFSW
                     GCALACSYLLDRKPFGVKGVMLCAPYLSSPAWDADQRRNIALLPKDIRGAIEKGEKTG
                     DFGEAYESAMMAYYDRLRPPDNTP"
     gene            complement(20147..20590)
                     /locus_tag="PLQ77_00355"
     CDS             complement(20147..20590)
                     /locus_tag="PLQ77_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPL09161.1"
                     /translation="MKKRYLIPLGLLYLALSSCAGCLTEEPSVQLRRVSLHPRSLTEI
                     QLILELDVQNPNRFDLTIQSLQYKLLLNEREIGSGRLEKEILAPALSTTRIQAPVTAG
                     FKNWNESLRAVIADKDIPYRIEGEANVRTIFGSIAYPFSRGGKIE"
     gene            20734..22203
                     /locus_tag="PLQ77_00360"
     CDS             20734..22203
                     /locus_tag="PLQ77_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPL09162.1"
                     /translation="MAKIKWNWIWGIGITVVFLGLLAHRVEFFREKPPESPEPQTIEI
                     ARPPESWMNIYQNRRKIGVIHRTFTALENGRFQTRENVAMQINTMGVVQALNLSTESE
                     LYPDMTFSSFHFEINSSLFRFAARGYAGQDRLIVFAGLPHAQKKTVLPLKDLPHISGN
                     IYEAAFRGGLEKDQTRSFSIFDPSTLALRSVKVTRFADEVIPIMGKRILTQKFCADFM
                     GAQNCAWLAKDGEILKETGMLGLSMEKVDAQKAREGISAEGIADFSQMASIASNRILP
                     EPSTLTQIVIRVGGVHNLPLMLSGGRQKFSSGVLTITQEETPDTAQPNNGPKLFQQYL
                     QPSPLVQSDAAEIQKQAAKIVSPKDSPPARLRKIVHWVYRNVEKKPVLSVPNALEVLK
                     NKAGDCNEHAVLTAALLRAADVPVQMETGLVYLKGRFYYHAWNTAYVGRWVTADAVFD
                     QIPADVTHIRLARGEGAEQLDLLGVMGKITLEVLSYKND"
     gene            22196..22921
                     /locus_tag="PLQ77_00365"
     CDS             22196..22921
                     /locus_tag="PLQ77_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09163.1"
                     /translation="MIKLINLTKDFKTTLAVNRLNLEVAAGEIYGFIGPNGAGKTTTI
                     RMMGGIIEPTDGKILIGGIDMSRDPVGAKKIIGFVPDRPFLYEKLTGMEFLKFIADLY
                     RVDLRVFSRRAPELLERFALWQWKDEIVEAYSHGMKQRLIIAAALLHDPRVMVIDEPM
                     VGLDPEAVRMVRDILKGLAQQNVTIFVSTHTFSLAEDLCDRIGVIHKGTLLAQGTVAE
                     LNLAAKTGEARLEEVFLTLVREL"
     gene            22926..24623
                     /locus_tag="PLQ77_00370"
     CDS             22926..24623
                     /locus_tag="PLQ77_00370"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09164.1"
                     /translation="MQSTVWILLKPRLLSARNGLKPNEQNGNRSRLVLFSLIGVTFWA
                     GAFAIFYRVLRYFQNVQDFGDILAMKLLSMMIITFFTLLLFSSIINSLSHMYLSRDLS
                     LLHSLPVDVRDAFLSRWLGSTFDSSWMVVAFSLPVFLSYGLVYQAGLAFYPIALSAIV
                     CLCLLASAASSLLVLLGAAILPAGKIRTLFVIMGVGLALLLILVLRMIRPEQLVNPDS
                     FASVVLYLNSMQALDWPWLPTTWITDTVRLALKREWGPSLFSLTLSASCFFSLMFFTQ
                     TAAGALYFRGFSRAQTTAKRLFTPVKFHGYSWETLLNFLSRPARAFAVKEIRSFFRDS
                     TQWPQLFLICALIVVYLYNFSVLPLDKSPMKTVYLQNVFSFLNVGLAAFVLSAIAARF
                     VFPAVSMEGEAFWIIQSAPVSMKTFLWIKFFLYYIPLLMLSEILIAASNILLGVTPFM
                     MFLSTVTIFCLVPAIVGLAVGLGARYPNFKSENPALSATSFGGVLFMLLSFGLIALVI
                     MLEAGPVYRIFMSRMHGHAFSLLQILWFILSFCLALFLCVLAVFYPMRQGEKNLLKR"
     gene            24787..26868
                     /gene="fusA"
                     /locus_tag="PLQ77_00375"
     CDS             24787..26868
                     /gene="fusA"
                     /locus_tag="PLQ77_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL09165.1"
                     /translation="MAKYEPKQLRNLAVIGHGGTGKTSLCESLLFITGKNERLGRVDD
                     NTSTMDFEPEEQKKHVSISSAVNFVEWDKHKINIVDTPGDSNFTFDIQCSLSIVDNAV
                     VVIDSVGGVEFQTQKVWEIANGYSLPRLIFVNRMDRERADFNKVLESIKTKLKKKATP
                     VCLPIGAEDKFQGIVDLIAMKAYTFADNKGAGKAVDIPADLADEAAELRSSMVEDIAE
                     ADDELMNKYLEAGELSAEEIMSGLRKGVIAGTLLPVICGSAVKGIGVTLLADLAVSSF
                     ASPVDRGPVKGKKPQTDSVEERQPSEDAPFSAIVFKTIADPYAGRLTLFRIYSGKLNS
                     DSAVYNSTRKITEKFGHIFFLEGKNQKQAETLIPGDIAGVAKLKETVTGDTLCAEKYP
                     IIFEKVAVPPPIMSFAVEPKSRGDEDKIASSIHRLTEEDPTIVFSRNVQTKEMILSGM
                     GQVHIEVNIEKMKRKFGVEVNLNTPKVPYLETIKGKTNVQGRYKKQSGGRGQFGDCTI
                     DIEPLPRGGGYEFLDKIVGGVIPGQYRPAVDKGIQEAAAKGVIAGYPVVDFRISLVFG
                     SYHTVDSSEMAFKIAGSMAFQKGVMECQPILLEPIVNIAIEVPDEYMGDVIGDLNSRR
                     GRVLGMDTNGTNQVIKGQVPLAEILKYAPDLRSMTSGRGNFTYTDSHYEEVPSYIADK
                     IIAESKKEAED"
     gene            26868..27362
                     /locus_tag="PLQ77_00380"
     CDS             26868..27362
                     /locus_tag="PLQ77_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPL09166.1"
                     /translation="MFNAGIITVSDKGSQGKREDKSGPVIAEMLAGAAIQIKQTLIIP
                     DEVAQIAKAIIQFADVEKLDLILTTGGTGVSPRDLTPDATLQVLDKEIPGIAEAMRAA
                     SMKVTPHAMISRAAAGIRGRSLIINLPGSPKGARENLSVVMPALTHAMEKIQGDDRDC
                     GVLP"
     gene            27403..27600
                     /locus_tag="PLQ77_00385"
     CDS             27403..27600
                     /locus_tag="PLQ77_00385"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPL09167.1"
                     /translation="MEPNVGPIDRIARLVIAVLFIVAVIADWIGGIAGVVLCVIAGML
                     LSSVVSGYCPLYVKLGLKKSF"
     gene            27606..29576
                     /locus_tag="PLQ77_00390"
     CDS             27606..29576
                     /locus_tag="PLQ77_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09168.1"
                     /translation="MGSRENQTRRGAGPEEALRERAGMLESILNGVLLLITYTDKDER
                     YAFVNRAYAEWYHIKPEELIGKRVSEVLKPDVYARAFENIQKALGGERVAYENQVTDP
                     EGRERILSVQYEPHFEGEAVKGFFTSIVDITGQKSAARELKERERVLATMIANLPGFV
                     YRCANDHDWTMTFLSEKCADVTGYTVSDFLQNKINYNDIVHPDYRESLREKWQEAILR
                     KGFFEEEYPVVAASGETRWVWERGRGVFDPAGELLFLEGFIGDITDRKRDEKEKEDLQ
                     TQLIQAQRMESIGRLAGGIAHDFNNMLAVILGRADMAMMRMDPAQRLYRDLQEIRKAA
                     ERSANLTRQLLAFARKQTVALKVLDLNEMAEGMLKILRRLIGENIDLVWQPGRNLPPV
                     KVDPDQMDQILVNLYVNAKDAIGGVGRIIMKTGAAALDEAFCAVHRGFVPGDYVTLSV
                     SDNGCGMNQETLNRIFEPFFTTKDTGRGTGLGLSTVYGVVKQHGGFINAESEPGRGST
                     FTVYLPRHLKAGIETAGNKETASVAGTETVLLVEDEPAILEMARMMLQRLGYTVLSAG
                     KPTEAMELARSHTGEIHLLMTDVIMPEMNGRDLALSISFFHPKIRRLFMSGYPANVIA
                     RQGVLDEGVHFIQKPFSMKDLAIKLREAIENR"
     gene            complement(29607..30098)
                     /locus_tag="PLQ77_00395"
     CDS             complement(29607..30098)
                     /locus_tag="PLQ77_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPL09169.1"
                     /translation="MNTPTRFLADAALSGLARWLRLLGFDTVCVQEKAGRPLMRQAAG
                     EDRILLTRRRDLLERQFSGRLLLLPDTAVAKQLSFVLGKLSLEIEARNLYTLCLSCNQ
                     KMISLERRNVRDLVPEYVFEHCAEYTQCPECRKIFWPGTHRRNALRFLKDNGILVSGA
                     EGL"
     gene            complement(30095..30412)
                     /locus_tag="PLQ77_00400"
     CDS             complement(30095..30412)
                     /locus_tag="PLQ77_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPL09170.1"
                     /translation="MKIFYLILTIVFAFFIVTFSQFNSAPVTIKYYYLQDLVVPAYML
                     IFVALLVGVVITGFLGVVERFRLNRTISKQNKVIRDLRKELREHEAPPMIEDSKKTHV
                     PSA"
     gene            complement(30419..30865)
                     /locus_tag="PLQ77_00405"
     CDS             complement(30419..30865)
                     /locus_tag="PLQ77_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPL09171.1"
                     /translation="MCEKPEGCVFCKSSLRCDDYVVLEGKTCFVMLNRYPYVNGHLMI
                     IPLRHLGDLAALNPEERAEIFSLVDVSVKVLKEAMNPQGFNIGMNLGKAAGAGIAEHI
                     HVHVIPRWEGDTNFMSVVGNVRVIPQDLAATAAALIPLFEKYCREV"
     gene            complement(30935..31429)
                     /locus_tag="PLQ77_00410"
     CDS             complement(30935..31429)
                     /locus_tag="PLQ77_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPL09172.1"
                     /translation="MKENKVKIGVLSDTHVHGFDPNLKRIIDENFSEVDLIFHAGDLV
                     DVSVLQLFGEKDVKAVCGNMDGPQVRERLPEQMIMDIGGFRVAMIHGWGSPAGLEGKL
                     AQKLGKVDCIVFGHTHYPVNKKIDGIYFFNPGSAMDRRFARHRSVGILEMGFEINGSI
                     INLK"
     gene            31581..33608
                     /gene="ligA"
                     /locus_tag="PLQ77_00415"
     CDS             31581..33608
                     /gene="ligA"
                     /locus_tag="PLQ77_00415"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPL09173.1"
                     /translation="MDKETAHRRIFELRKTIEYHNARYYQQDAPEISDAEYDRLMKEL
                     QNLEALYPDDELASSPTQRIGAAPLSKFAPFAHPQPMLSLANAFGADEIKDFDSRVRR
                     LAKSERVDYVAEPKLDGLAVNLIYENGRFRRGATRGDGAVGEDVTQNLKTIASLPLKM
                     KTGAFRPIPEFIEVRGEVYMEKEPFEKLNRRRVEEGDAPFANPRNAAAGSLRQLDSKI
                     TARRPLNIFLYGVGSAGDAAFASHWEILETLAAWGFPVNPLIRPMPDINACIEYYEQT
                     GSIRSSLPYDIDGVVLKVNRLEIQNLLGSVSRSPRWALACKFPAQQAATVIQDIIVQV
                     GRTGTLTPVAVMEPVNVGGVLVSRATLHNEDEILKKDIRVGDAVLIQRAGDVIPEVVS
                     VILSKRTGREKEFRMPRACPECGSEIVRQAGEAAHRCVNLSCPAQLKEHIRHFASRGA
                     MDIEGLGEKLSAQLFDAKMIADPADLYFLTKEKLLGLDRQAEKSAENLLEAVERSKTP
                     ALDKFIFALGIRHVGERTAKLLAGHFGSLEALMSAGCEELTAIREIGPEIAQSILEFF
                     AEPKNRIVLEKFQRAGLSPRTPAPTAQASLQGKSFVFTGSMESMGRNKAKAAVESLGG
                     IVHSSVTGKTSYVVAGSDPGSKLEKARKQSIPVIGEEEFLTMIGRQIHRQA"
     gene            33655..33930
                     /locus_tag="PLQ77_00420"
     CDS             33655..33930
                     /locus_tag="PLQ77_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPL09174.1"
                     /translation="MKRMHVLISGRVQRVFFRAETLRTALSLGLAGWVRNMEDGRVEA
                     LFEGEDESVDRMLAWCRQGPLHARVDRVDMTEEPCRGDLQGFRIKDF"
     gene            34072..35676
                     /locus_tag="PLQ77_00425"
     CDS             34072..35676
                     /locus_tag="PLQ77_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL09175.1"
                     /translation="MSLADRNNPYSFNEFLAWRQGADYYADDPFIQKVVRHYTGDIRR
                     EVDAEARAVSAKASFRWRDMAERIARPEERPYILHYDGHHNRIDRIVRPRDTEIMEKE
                     IFSEAFFAEKTPPWVKLIKMYIIYQNGEACIGCPVTCTEGLVELLNKYADSPETKEIL
                     RHCKEGIDGDFAIGAQYLSEIQGGSDVPSNVLEAVRENGVWKLYGTKFFCSATHADYA
                     VVTAKPAGSEKVGLFVVPSWLAGSKEKEIRNGCTIDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRAVREAEKYVSFRDAFGLRLNQF
                     PLVAVQLDRIRKTTRRTTAGAFKLYRDFLKLEGGLKGSRGDDEPLPARRHRFNIRELI
                     MLQKIAASWDCTDVIREAMSLFGGHGVMEDFSCLPRLYRDSAINELWEGPRNVLLTQI
                     HRDLQKAAGWYKPEEFVAEILQGLDKAQVQEMAEEIKALVAHPHLFGMDEATIDVCRR
                     WDAFCHRFSHAYQDLAVKEMDSPLKG"
     gene            35861..37654
                     /locus_tag="PLQ77_00430"
     CDS             35861..37654
                     /locus_tag="PLQ77_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPL09176.1"
                     /translation="MMFLLKQVVHRLRSIFLENIARWLVFGLLVGIVSGLGASAFYYL
                     LQAGRHFCFNLLAGYALPAPAGEQLFEAAGTLQFHRLVFFLLPALGGLISGLLVYHFA
                     PEAEGHGTDAMIDAFHNKKGRIRTRVPLIKGLASIITLSSGGSAGREGPIAQIGAGIG
                     SAIARLLRLSDRERRILLLAGCGGGLSAIFRAPLGGALTAIEILYKEDMETEALVPTV
                     VSSITAYIIFTYFFGNAPIFFFPEYTFSNPRELIFYILLGLICVPAGIFFIKVFYAVR
                     ERIFQPLKIPLYVKPALGGLAVGCIGLFFPQVYGDGWGWIQLAILGKLGIGIMMAIAL
                     AKIIATSFTISSGGSGGVFGPTLFIGGMIGGAVGYSAHYFYPNIVAQPEAFVVVGMAS
                     FFAGVASAPIGTLLMCSEMTRGYGLIAPLMLVSIIAILFTRQHSIYARQVQSKRESPA
                     HTADFTVNLFAETRVRDFYKPEKVTPVRKTTSYGQLKKIFSASRIECFPVYDENDALI
                     GSVNWDHARTIVFEEGLEDLIIAEDMMSPVRTVTPEDNFYDAFMKFMETNAEELLVVS
                     PDDGNIVLGVLRHDDLIAAYSAELNRRKNSG"
     gene            complement(37673..39037)
                     /locus_tag="PLQ77_00435"
     CDS             complement(37673..39037)
                     /locus_tag="PLQ77_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL09177.1"
                     /translation="MAKILVLDDDQGMREFMEIMLTKEGYDVTCAGKPEKAISLCRKT
                     AFDLVITDLKMPKIDGIEFLKTIKEQRPETVVILITAYASGETAVNAMKEGAYDYVEK
                     SGNIDELKKIVRQALLQQGSPGEAQTPKKNPPAETSFCGMIGASREMAKVFSTIRKVA
                     DTPANILILGESGTGKELVARAIHENSSRAPMPFMAINSGGIPENLLESELFGYMKGS
                     FTGAYGDRAGLFELARGGTVFLDEIGELPPVLQVKLLRVVQQKTFRRIGGDKDITVDV
                     RIISATNQNLKEKVARGSFREDLYFRLNVIPIHMPPLRERKEDIPLLTRHFIEKYARD
                     FGKEVRTISSYAMELLMEYPFPGNIRELENIIERGVAMETSNIILPENLVLTTDADAP
                     GVSIDLSIPDGGIDLNEEMNRIEKRAIEKALQKTRGIKTKAADLLGVTSDSLKYRIEK
                     LGIE"
     gene            complement(39056..40714)
                     /locus_tag="PLQ77_00440"
     CDS             complement(39056..40714)
                     /locus_tag="PLQ77_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09178.1"
                     /translation="MRQPMQYDERTWRRLKLLIVSRVVILSAFLLVTLFLGTVKSIIF
                     ISRDMLHAIYAVIVAIYILSVVYTLLLRKKKYFQINIYFQLAADIVLVSFLVYLTGSI
                     GSNYSLLYTLVIIYAAIFLGRRGALIFASVCSIAYGLVLDLEYFGVIPTFYATGRDYT
                     LEAGDVFIRIAVHILSFYILAFLASFVVEQEKKARSLLAEKESAFDQLDLLFRSIIES
                     VDTGILTTTLQGRIKTFNRSAEKITGHPLRSVENRQIEDVFPAFAPLFSNGDGPQMKP
                     KGEIKINSQKGVPIYLGYALSPLKDKNSGQIGHILIFQDMTEIRRMEENLEKSRRLAL
                     IGEMAAGLAHEMRNPLASITGSIQLMAQTPNLDETNKRLMQIILRGKDRLESFVRDFL
                     LLARPVPEAKQFVRVNDVAEEVVDSMKLSADWREGIQLEKRISGDPQALANEEQVRQI
                     LQNMLTNALQSMEGKGRIDLEIRTVRLEDKRDYAEIKVTDDGCGIEEKDMKKVLEPFF
                     TNKERGTGLGLAIVSRIVDGYGGRITIRSRVNEGTAITVWLPCR"
     gene            40822..43344
                     /locus_tag="PLQ77_00445"
     CDS             40822..43344
                     /locus_tag="PLQ77_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPL09179.1"
                     /translation="MRRPLWYPLAALMAGIWIGEGISPAPAVLPVCMLPVLTLLLVSV
                     SRRWNRAAFLLLLALMLTAGMLNIQRQPFLAGQDRHILHSAGQGKTTLEGIVLSTEQA
                     LPERNTLIVDCRRVLRDSTAVPATGKIRLAIPPDLSFQYGDFIRVHTKIREIQSFHNP
                     GSFDYERVQNRRGIYVAGYVSDRAGIVLIRPGAAGGLRLKLERFRLRLKRLIEARAPS
                     PQREILQALTIGNQKSIPQNVRDDFAKTGTSHILSISGLHVGIVAASAFFLILIALKS
                     SEYLMLKFNIIKAATGAAMVPVLIYALVAGMGTPVLRSALMTLAFLAALLIGRSKDLY
                     NILAGAALLILIVLPEALFEISFQLSFSAVFAILYAVDKLGNRRAPLPSSTPRWISAG
                     IGRLYLFLLVSVAATLGTLPIIVFYFNRVSAVTLIANLVAVPLLGILALIPAMLFILT
                     SLISEALAGFFISVSSFFTGIAVRIIEELASLPWSSFSFVKPNVMEIALFYLFLYFLV
                     QALSSGSGKDHSTRRPILMRTGLMICVALILADAAFLVLKDRYSTHLKITAIDVGQGS
                     STLLQLPRGVNMLIDGGGFHDSSFDMGRAVIAPFLYAKRIRKVDIAVLTHPHPDHLQG
                     LIYILNNFDVREVWCTGLTADDDLYRLWKNTISGRGVKVRNLSAQSPPVNLSGVNFRF
                     LWPPLPPDPVGRALSYEDTNDASLVMKITYGTRRFLITGDISASVEARLIASGEDLKS
                     DVLFVPHHGSATSSSPRFVRAVSCRWAVVSAGKNNVFRHPHTAVLERYRSAGAGIFRM
                     DRDGAVSIDSDGRALSITPWIKRPEPEPAGRRALNISLPMPG"
     gene            43382..44656
                     /gene="hisS"
                     /locus_tag="PLQ77_00450"
     CDS             43382..44656
                     /gene="hisS"
                     /locus_tag="PLQ77_00450"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPL09180.1"
                     /translation="MEKITAVKGIKDVLPGDSPVWHRLELAARRVFECFGFCEIRVPI
                     MEKTSLFQRSIGEATDIVEKEMYTFRDRDDELLTLRPEATASVIRAYIEHNLSASDPV
                     TRLYTMGPMFRRERPQKGRFRQFHQIDAELFGDDSPAADAEVIFMLMHFLQTLGIGKL
                     SLEINSLGCKACRPVFSGAVVDYLKGRESSLCPDCQRRIHTNPLRVFDCKVESCGEVI
                     DGAPRILDFLCADCSEHFSRVKSYLSDLNLPYEINPRMVRGLDYYTRTAFEVKSGGLG
                     AQNSLAGGGRYDGLVQFLGGPDIAGIGFGIGMERLIACLPPDDRNPYAAEIFIAALGE
                     RAQKIAFTLTQELRRRGICAAMDYSGKSLKSQMKRADKLHSAYTLIFGDREIEENCAE
                     LRDMNTKNQQRLPLDQLLEAVTDILKREVKVL"
     gene            44668..46425
                     /gene="aspS"
                     /locus_tag="PLQ77_00455"
     CDS             44668..46425
                     /gene="aspS"
                     /locus_tag="PLQ77_00455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPL09181.1"
                     /translation="MKEKRTHYCGSVTVADEGNEIVLMGWVHRRRDHGGVIFVDLRDR
                     EGIVQVVFQPDAGEAVHGEAHKIRSEYVLAVKGTVRRRPEGMDNPALPTGQVEVVISE
                     LEILNESKTPPFSFDDEDISENVRLKYRYLDLRRPAIQKNLFLRSRLAIATRQYFNDH
                     GFIEVETPFLTKSTPEGARDYLVPSRISKGMFYALPQSPQIFKQLLMVSGFDRYFQIV
                     KCFRDEDLRADRQPEFTQIDVEMSFVSEEDILRMMEGLMAAIFKACRNIDLPLPFPRL
                     TYAEAVSRYGKDNPDVRFGMEITDLTAIVKDSGFKLFAEVAASGGVVKAVKADQASSL
                     SRKDLDALSDFVAIYGAKGLAWAKVNAADWTSPIFKFLKPEETAAIGRAMDAKEGDIL
                     FFVADTPKTACDALGNLRLHLGKKLNRIDPDALAFTWITEFPLMEYSESEKRFVSTHH
                     PFTSPFLEDIPLMTTDPGKVRARAYDLVLNGSEIGGGSIRIHRKDIQAQVFSALGLSD
                     EEARLKFGFLLDALEYGAPPHGGLAFGLDRLVMIMTGAESIRDVIAFPKTQKAADLMS
                     DAPSKVSIEQLMELSLKIV"
     gene            complement(46422..47681)
                     /locus_tag="PLQ77_00460"
     CDS             complement(46422..47681)
                     /locus_tag="PLQ77_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL09182.1"
                     /translation="MKIGQKGGWFTLIDQIAARCLKCKVPQCRKHCPVSTPIPDVTRL
                     VEEGKEQEAAQILFANNPLSAVCSIVCPHERNCFGHCVLGKKSEPVDFFKIEQFLSWT
                     FLKTYEPPVMEKNGARIAVIGSGPAGITMSILLSLMGYRVTLMEARDRIGGVLRYGIP
                     GFRLPREIVDRYDPILRQLGVQFKPNTFIGSSITLDDMFIDGYKAVFIAVGTAKPNKL
                     GLLGETLGNVFYAIDYLKSPEAYRLGRNVVVVGAGNVALDAARIAVRSIEPGGAVTVI
                     NNLLEEEIFGNRQEIDDAVREGIQFVHLLQALKLEEDGVKCVAVRAEKTESGTVYEED
                     FSRTQKIPADSIIVAIGQGPQGAVMADSNVSKTHRGLVAADENGHTSSPGVFAAGDIV
                     TGPKTVVEAVAFAKKVALEIDRYCREN"
     gene            47837..48337
                     /locus_tag="PLQ77_00465"
     CDS             47837..48337
                     /locus_tag="PLQ77_00465"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPL09183.1"
                     /translation="MKEMEYLNAAGEAMRKIRKGAFLTVRAADAQNTMTIGWAMLGVI
                     WQKPVLMVAVRNTRHTFGLMEKAPDFTVTVPSGDMSRETAFCGTKSGRDLDKFAMCPL
                     KTAKGRHVASPIIQTPGMHFECKKMYQSAMNPAHLDSGLDQAIYPKKDYHTLYFGEIL
                     ACYETE"
     gene            complement(48368..50332)
                     /locus_tag="PLQ77_00470"
     CDS             complement(48368..50332)
                     /locus_tag="PLQ77_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPL09184.1"
                     /translation="MIEYAKELNPEQYRVVTEEGGPLLVLAGAGSGKTRTLTYRVAHL
                     LESGIRPENILLATFTNKAARSMLNRVESLVQHYTGKIMGGTFHSIAHRILRSYAFRL
                     GYNSSFSIVDSEDARQVIGSAMAELKIDTKLTKFPKNNIIAEMISLALNTETALEDVI
                     SSRYPFFLHLSAEIHQIACLYDQKKKDLSVMDFDDLLANCRRLLREQPDILQTLSRRF
                     EHVLVDEYQDTNILQADIVDLLASGHRNLMVVGDDSQSIYSFRGANFENIIHFPERYP
                     DCKIFKLETNYRSTPEILHLANLSINNNENQFPKALKAVRNKGMRPVIVSAQNVLKQS
                     DFVAQRISELSRSGVPFCEMAVLYRAHYHSMEAQMEFVRRDIPFDIRSGIRFFEQAHI
                     KDVTSYMRVLVNPHDELAWRRLLMMYPKIGKITFEKIWSRLKKQDHPLETLISESFLQ
                     TAPKSAQNGVGQCRKTLLLLLALDVPDRTPEKIIDLLLNKGDYRTYLQDSYSDAYARE
                     EDLIQLGNFAARFERMDDFLNELALLTNMSKEEEIEERQEDKVILSTIHQAKGLEWSY
                     VFIIGCADGMIPLERALREPGGEEEERRLFYVAVTRAKDQLYLSYPVLDYSRSAGVQT
                     LCPSRFLSEISPLSPQDEHRPFEQWALFDD"
     gene            complement(50347..50994)
                     /locus_tag="PLQ77_00475"
     CDS             complement(50347..50994)
                     /locus_tag="PLQ77_00475"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPL09185.1"
                     /translation="MRIILLGAPGAGKGTIAKLLTEYDGSIQISTGDILRSAVKEGSA
                     LGKKAKEYMDRGDLVPDSLIMDIMEARLQEPDCQKGFILDGFPRTIPQAEDLQKLLSK
                     LKIKLDFVANLDVPRDVILDRLTTRRTCCNPACQAIYNVKSNPPREDGTCTLCDHQTI
                     QRDDETEEAITNRLETYNLKTAPLTGFYEKEGLLKTFLSLSSKDTVAEIKKALHA"
     gene            complement(51074..51898)
                     /locus_tag="PLQ77_00480"
     CDS             complement(51074..51898)
                     /locus_tag="PLQ77_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPL09186.1"
                     /translation="MTRNIPKIFAALLLLSLCVLLPCLTGAERIPPGKNPFSLLMKTK
                     APISAVAVQKIHPHDSDAFTQGLIVMDGVLCESTGLLGRSTLACKDIDTGKVLRQIQL
                     PPEFFGEGIALLKGRIYQVTWQNENALVYDAASLREIRRMKYSGEGWGLTSDGKSLLM
                     SDGSSIITIRDPKSFQVQRRIRVQDGDRPVSRLNELEFVRGEIWANIYMEDLIARISP
                     QTGRITGWIDLSALRSYLPANARVDVLNGIAFDAKQNRIWVTGKYWPHLFEIQITP"
     gene            complement(51982..52674)
                     /gene="tsaB"
                     /locus_tag="PLQ77_00485"
     CDS             complement(51982..52674)
                     /gene="tsaB"
                     /locus_tag="PLQ77_00485"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPL09187.1"
                     /translation="MLTLAFDTSSKTVSVALLEDRAVLYNAILHREQNHSEVLLPAID
                     EACRQTGISVRDIGFFACTLGPGSFTGLRIGLSTLKGLMMATGKPAAGVSSLAALALN
                     AEEPDAVIGSMMDAGRGQVYLAYYRRGKDGVLQPVRAPQAQDPRSVEDPFSEPVIYVG
                     DGAVRYAGLIPPLSSPKNKMAPVEQQFNSAAAVARLALEKYFRKDFLDPARCAPLYLR
                     SVDALPVAGSSG"
     gene            complement(52676..53767)
                     /gene="rseP"
                     /locus_tag="PLQ77_00490"
     CDS             complement(52676..53767)
                     /gene="rseP"
                     /locus_tag="PLQ77_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPL09188.1"
                     /translation="MVTFISFIVLLGILIFAHELGHFLAARAGGVGVLKFSLGFGPKI
                     FGKKIGETEYVLSWIPLGGFVKLLGESGAEELPAEDEKRSFYKQPRWKRMLIVLAGPV
                     FNFLLAVFIFFLVYMHGLPNLLPVIGELSPDSAAASSGILKDDAILSINDREITFWDE
                     IKPVILKAGGADLTIRIRRGGEEKIFAVKPKLTKTKNIFGEEESTYLIGISPAGKTVI
                     EKKNPTAAMASAVEKTWEISKLTVISVVKMLEGVLSPRTLGGPIFIAQVAGNQVREGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHIFFYLIEMAARREIPVKIKEWASQIGFVF
                     LLMLMLFVIMIDIERLNIGPVNKVLQFFR"
     gene            complement(53772..54938)
                     /locus_tag="PLQ77_00495"
     CDS             complement(53772..54938)
                     /locus_tag="PLQ77_00495"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPL09189.1"
                     /translation="MKKITILGSTGSIGASALDVVRKNPNKFRVAALAAGRNVKLLAR
                     QALAFHPAAVSVRTPGDAKRLRGLLGPGGKIRVLCGEEGVREIAAHSAADIVLSAISG
                     AAGLKPTLAAIEAGKDIALANKETMVIAGHLVTKKAEQKKIRVLPVDSEHSAIFQCLA
                     GQKPANIKRIILTASGGPFYSFSRADLKKVTPAQALRHPRWKMGAKITVDSASLMNKG
                     LEVIEAKWLFDIAVSRIDVLIHPQSVVHSALELLDGSVLAQMGIADMRIPIAYALSFP
                     DRMTNDLPALDLAKAGPLSFFPPDTKKFPCLRLALEAARIGGTAPAVLNAANEEAVAA
                     FLENKICFHEIPNVIEKTLERHSAIPDPSLEEILLADGEARKVAAGILKKRKRG"
     gene            complement(54938..55753)
                     /locus_tag="PLQ77_00500"
     CDS             complement(54938..55753)
                     /locus_tag="PLQ77_00500"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPL09190.1"
                     /translation="MATHNSNLQRWMTGLALAAVLLLIIVMGSPVVLTAIVALVIVIG
                     LWEYNGIVFGPGHRAEKTESLLFAFLIPWAVLAGDARWIAAILTFAMLIVFMVFLWQV
                     RKSFPDPSSVMKVMFGLLYIPLLTSHFVLLRKLEGGIYWVLLVLVIGIIGDTVALYVG
                     KSLGKRKLLEAVSPGKTVEGTLGLIAGSTLAACVFGYFWLPGVHWIHFPVLGFVGGII
                     GQLGDLCESAIKRSHGKKDASDLLPGHGGLMDRMDSLIFLAPFVYYYRIFVMG"
     gene            complement(55773..56513)
                     /locus_tag="PLQ77_00505"
     CDS             complement(55773..56513)
                     /locus_tag="PLQ77_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPL09191.1"
                     /translation="MPEIDQNQLPRHIAIIMDGNGRWAKQHAMGRIRGHKKGAQAVRT
                     TVTACRELGISCLTLFAFSSENWGRPGEEVSALMNLLADYLDKEAPTMQKQEIRLTTI
                     GDIDRLYAPVREKLLEVQKLTRQNNKMVLNLALSYGSRDEILAAVKKMLADSREGKLS
                     PDSVDQDVFERYLYTSGMPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFKK
                     DDLLKAIAAYQSRERRFGLTSDQVSGRK"
     gene            complement(56589..57146)
                     /gene="frr"
                     /locus_tag="PLQ77_00510"
     CDS             complement(56589..57146)
                     /gene="frr"
                     /locus_tag="PLQ77_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPL09192.1"
                     /translation="MKEQIFSRFKDNMEKTISSLEKSFSRVRTGRASVSLLDGIKVDY
                     YGTPTPIAQVANLSVPESRLIVIAPWDVTTIGAIEKAIQKSDLGLMPSTDGKVIRLSI
                     PQLTEERRKDLVKVVKKMAEEGKIKLRNERRDANEALKELKKNNKMSEDEMHGAQDEV
                     QKLTDRYIEKTDKILAAKEKEIMEI"
     gene            complement(57149..57874)
                     /gene="pyrH"
                     /locus_tag="PLQ77_00515"
     CDS             complement(57149..57874)
                     /gene="pyrH"
                     /locus_tag="PLQ77_00515"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPL09193.1"
                     /translation="MSEKKKAAYQRILLKLSGEALLGKQSTGVDPDVANYIADEIRSL
                     ADLKIQIGIVIGGGNIFRGVQAGARGMERTTADYMGMLATVINSLALQSALEAKGVAT
                     RVQSSIEMHEIAEPFIQRKAVRHLEKGRIVIFAGGTGNPYFSTDTAASLRAMEIRADV
                     ILKATKVDGVYSADPVKDQSAVMFKKISYIDVLTKNLKVMDSTAISLCRDNGLPLIVF
                     NLQKKGNIRGVVCGKKIGTYVGE"
     gene            complement(57874..58527)
                     /gene="tsf"
                     /locus_tag="PLQ77_00520"
     CDS             complement(57874..58527)
                     /gene="tsf"
                     /locus_tag="PLQ77_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPL09194.1"
                     /translation="MREKQTQTKEFQEEKIVEITSTMVKELRTKTGAGMMDCKEALAA
                     VDGDFEKAIDFLRKKGLSAATKRSSKAAKDGTIASYIHMGGRIGVMVEINCETDFVAK
                     TADFQTMARDIAMHIAASNPLYVRPDEIAEDALNREKEIYRSQLREEKKPEKIWDKII
                     EGKLKKYYEDVCLVEQKFVKNQDITVGTLISNMIAKTGENIVLRRFARFQLGEEIKQ"
     gene            complement(58609..59418)
                     /gene="rpsB"
                     /locus_tag="PLQ77_00525"
     CDS             complement(58609..59418)
                     /gene="rpsB"
                     /locus_tag="PLQ77_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPL09195.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGMFQSAYNFVVDTVSQGREILFVGTKKQSQEAIREEATRCSMPYVNQRWLGGMLT
                     NFITIKKSIDRLNSLSKMFADDSVKAFPKKEITKLQKEMDKMDNVLGGIKNMRSLPAA
                     VFIVDPNRERITVQEAKKLKIPLVAIVDTNCDPTDIDYIIPGNDDAIRAIKLFSSKFA
                     DAVIEGRKRFEEKLSAESNKEEGAPAAENKDPEADIPESVEMENGSPAPAS"
     gene            complement(59600..60799)
                     /gene="argJ"
                     /locus_tag="PLQ77_00530"
     CDS             complement(59600..60799)
                     /gene="argJ"
                     /locus_tag="PLQ77_00530"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPL09196.1"
                     /translation="MTKSANKFSVPGFLANGVSAGIKGGDRKDLGLICSTVPARVAAL
                     FTKNTFKAAPVLIDAERVKRGTAQAILTNSGCANAATGKEGMADALAVSAAAAKQLKI
                     PEEHLLICSTGVIGRRLPVRKIEAGIGKLVKGLNESGIEDAEAAMMTTDKFPKISIRK
                     GIVGAKDITICGIAKGAGMIEPNMATLLTYVMTDALIDAKALNTVFHQVIDSTFNAVS
                     VDGCMSTNDTAIILANGLAGNNPLERAQARLQRFREMLTDVMADLSAAVVKDGEGATK
                     FITVVVEGAKSKSDARRVAYSIANSNLVKTAFFGEDPNWGRILSAVGASGILLEKEKV
                     SLSIGNMTVFAQDAPANIAPARLKEIFQGGSIEVRVSLGGGDKSYTVYTSDLSHDYVK
                     INADYTT"
     gene            complement(60821..63340)
                     /gene="secA"
                     /locus_tag="PLQ77_00535"
     CDS             complement(60821..63340)
                     /gene="secA"
                     /locus_tag="PLQ77_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPL09197.1"
                     /translation="MLNAILKKIVGTKNDRELKRLSVLLHEVNHHEKELMSLGDAELK
                     AKTSAFREKLKNGLTLDDILPEAFAVAREASRRTLMMRPFDVQVIGGIVLHEGKIAEM
                     KTGEGKTLAATMPLYLNALSGKGCHVVTVNDYLARRDAEWMSPIYHFLGLTVGVIVHG
                     MDDDERRKAYAADITYGTNNEFGFDYLRDNMKFSLEDYVQREFNFAIVDEVDSILIDE
                     ARTPLIISGASEESTDKYYKINQIIPRLRKDADYTIEEKSRTVVLTEEGVSHVEQLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKEGQVIIVDEFTGRVMPGRRYSDG
                     LHQALEAKEKVKIEKENQTLASITFQNYFRMYKKLAGMTGTADTEAEEFKKIYNLEVV
                     VMPTNMPMIRQDHNDLIYKTEPEKIKAVIEEVKALNKAGRPVLIGTISIEKSELLSKY
                     LTRAGVKHHVLNAKNHEKEAEIVAQAGQAGQVTISTNMAGRGTDIKLGEKVAELGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDNGSSRFYLSLEDDLLRVFGADRISSVMSTVGIE
                     EGQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNNQRKVIYEQRKKVLRGED
                     LWSDIEAMVEEIAGDLLAEFIGEKNHPQDWNLKSLEDAVFQQFKLRLNIANAQDLTSA
                     DAVRSLILEKVQEYLRAKEKEFGPELMTYLMKVIMLQAIDTHWKEHLLSMDHLREGIG
                     LRGYGQKDPVREYQREGYAMFMEMISRIKMDTLEKLCLVRIQREEEVEEIRQKQKQDY
                     ILSRGEDTPVNQTVKRATEKIGRNDPCPCGSGKKYKKCCGA"
     gene            complement(63505..64359)
                     /locus_tag="PLQ77_00540"
     CDS             complement(63505..64359)
                     /locus_tag="PLQ77_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPL09198.1"
                     /translation="MTKISLSGKLIYGLIAGSILAVLLTLYIIYDYAGITRDRMELSR
                     LRQQTAQQTKEMNELAIKIDQFADRMEEIKQFDKKLRILATEQIGQDAKTPLGIGGSN
                     NDQMRLRQLIEEDREKLVSGMHENIDRLNAEADEREMSFNELLRFLREQKSLLAATPS
                     LWPVHGWVTSEFGMRRNPFGSGGEFHKGIDIASRAGKEIIAPADGFVLEAAYRTDDGN
                     VVKIEHGYGISTSYAHLSKAAVKDGVQVRKGDVIGYVGDTGRSTGAHLHYSVFVNNVP
                     VNPRKYLK"
     gene            complement(64575..65432)
                     /locus_tag="PLQ77_00545"
     CDS             complement(64575..65432)
                     /locus_tag="PLQ77_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL09199.1"
                     /translation="MGRLGLKIDVDTYAGMKNGVPRLLRVLNDFSVRGTFFLSIGPDN
                     SGRAALQMIKNPLFLKKMLRTRAPSLYGWKTALYGTLLPAPMIALSFPGLVRQILAEG
                     HEVQFHAWDHRRWQDDLPEKSEAWIEEWFDRGLKGFEKLTGQKPAAFGAPSWLIDDRV
                     LRIIRKYPFAYLSCTRAKAPFVHDPLNLPEIPSDLPCFEELDLQNGPAVILGLMNDGG
                     DHVLPVHAEVEGGVFEEQFRRLLQASLKAGMEVTGLGDFKNALTMDRLPRRKYKMELL
                     PGRHSLCAV"
     gene            complement(65456..66490)
                     /locus_tag="PLQ77_00550"
     CDS             complement(65456..66490)
                     /locus_tag="PLQ77_00550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HPL09200.1"
                     /translation="MKILILGVNGFIGHHLVNRILAEKPDWQVFGMDLGTERIGAALH
                     NPRFNFLEGDISINREWIEYHVKKCDVIMPLVAIATPKAYVEDPIGVYNLDFEMNVNI
                     VKQCVKYRKRVVFPSTSEVYGACSDPEFDEDESYLSVGPIRKERWIYSCCKQLLDRVI
                     YAYGTRGSLEFTLFRPFNWIGPRLDSLDTAKEGSSRVVTQFIAELLLKRPINLVDGGR
                     QKRCFTYVDDGIAALMKILENKGDVCKNQIINIGNPDNECSVKELAALLKKLFQEHPD
                     HQQDSVYSDIIEIPADVYYGKGYQDIYTRKPRIEKARKLLGWEPKIGLQESLRLTLNS
                     FLEENQAATL"
     gene            complement(66487..67404)
                     /locus_tag="PLQ77_00555"
     CDS             complement(66487..67404)
                     /locus_tag="PLQ77_00555"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HPL09201.1"
                     /translation="MKAIVLAYHNMGLAGLAALGRHGFDIAAVFTHEDDPKENCWFGS
                     VKDWAEKQGIGCTTDPVNSPQWIEKIKRMQPDVLFSFYYRNMVRPEVLAIPRIGAFNL
                     HGSLLPSYRGRCPVNWVLVNGEKETGVTLHCMVEKPDAGDIVGRRAVSIDFEDTARTL
                     YDKLCEAAGGLLDELLPEMKKGKIPRIKQDLSRGSYYGGRKPEDGRISWDRTAVEIYN
                     LIRAVTDPYPGAFAFAENGGKILIWRARPVSSVSGGGPGDVILDGQSVLVKTGDGAIR
                     LQEIEASGARWKDAGIGNYFKQGKVKKLT"
     gene            complement(67401..68351)
                     /locus_tag="PLQ77_00560"
     CDS             complement(67401..68351)
                     /locus_tag="PLQ77_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL09202.1"
                     /translation="MSEIRISVVIPVYNEEANIQALMGRLLPVMESLAKPFEIILVDD
                     GSRDRSLALLKEFTSHPSVRVVELTRNYGQHAAIMAGFSVMRGDIVLTMDADLQNPPE
                     EIPNLVRTMEEGEYDVVGTIRKSRKDSFLRILPSKMINVVARKITGVSMRDWGCMLRA
                     YSRPVVERMLDCHEQATFIPALATVFAKRVTEIEVAHEERFGGKSNYPLRKLINLQFD
                     LVASFSDFPIKLIMYGGILMSLLGVCFGAFLAIARLVYGVRWAAEGIFTLFAILFVFV
                     GLQFFALGVIGEYIGRIFREVRKRPEFVIERVYGHEGDRP"
     gene            complement(68344..69495)
                     /locus_tag="PLQ77_00565"
     CDS             complement(68344..69495)
                     /locus_tag="PLQ77_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPL09203.1"
                     /translation="MKIRKDFLPFSRPSIGETEINGVVACLKSGWITTGALCKAFEEQ
                     LCALTGADRAVSLSSATAGMHLMMKALNIGPGDEVITPSMTFASTVNMIALHQAKPVF
                     VDIDYGTLNLCCEEIEEKITKRTKAIIPVHFAGAPADMDRINRLARKYSLKVIEDAAH
                     AVGTYYKGTHAGGFGHPAIFSFHPIKNITTGEGGMITLNQAALEKKLRLLRFHGIERD
                     AWKRYGKGGNPSYDIAVPGYKYNMPDLLAALGLAQMERWRELNARRQMLAGLYLEGLR
                     GVKGLDLPEVPSYDHVHAWHLFIVKVGGIARDVFMQKLSQYNIGYGLHFPPAHALSYV
                     KKKFGVKARSLPQTSRAAGRVISLPLFPDMTENDVHYVCGAIKEILKNV"
     gene            complement(69502..71181)
                     /locus_tag="PLQ77_00570"
     CDS             complement(69502..71181)
                     /locus_tag="PLQ77_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPL09204.1"
                     /translation="MKKKYILFIIPFLLYVALLGVLPQMEPDEVRYSLIAAAMNETGN
                     YVTPQIKNTIYLEKPPLVSWATAASFKIFGENDFSARLFSGLCAWGCILLVFWIGRRF
                     RDEKTGLYAAAVLTISVFPFMLGRINILDMPLTLFLCLAIWLGYLSLADEKKRWLACG
                     FYFACALAFLTKGIVGVVFPFAILVIWLIREGRWRSLPRLISPAGILIFLAVVCPWLY
                     LAQKENPDFLWFFFVREHFLRFTTQMHGKTEPFYYFLPVLIGGTLPWSVFLIRAWQPA
                     KWKERLFRKEENRFLAVWFLFVFLFYTVSSSKLATYIAPVFLPLALFAGNIFRGHEEA
                     PSEPAGKREVFYRLLLWGQFLVIAAAVILPPVLKQYHDPSRGLVIMISNAWWICVLPV
                     FLAAGLMTFLPDWIFRRKGAGWFSTMYLLCCLLLAGLLFPLNDFLSPYRSALTAKEAI
                     ARHIPPDRLLYQYRVNFYGIDFYNKIRTPIVEDFGELGDGIMKMPEAERKAYFLSTQE
                     FFEKGRREKEIYCITQHAERLKELQAQFARMEVLWENGAFYLLRIHNQAKD"
     gene            complement(71267..71893)
                     /locus_tag="PLQ77_00575"
     CDS             complement(71267..71893)
                     /locus_tag="PLQ77_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPL09205.1"
                     /translation="MKKRWIALALIASGAALAGIAYAFWDIPLVHFCRGLGRPVLDAT
                     EWITMAGESRWYFMIIVPALLIFWLIAKNKKWTIRMFFLLVCISASGLINLLLKWIAG
                     RNRPVNLFDHGLFGFDFFETGYALNSFPSGHTVTVFSLAAALTILFPRFGAWAFLPAA
                     VIAASRVMLTSHYLSDVAAGAVVGVVCTLGVKYFFDRAGAGLDSGTRQ"
     gene            complement(71890..73335)
                     /locus_tag="PLQ77_00580"
     CDS             complement(71890..73335)
                     /locus_tag="PLQ77_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPL09206.1"
                     /translation="MPKAEKSIIILILFFSLIRLIAAPLFGLGVDEAHYVLYAKYLDW
                     SYLDHPPLAGWLHAPFYYIFGTNEFLARLPAVLLFAATSWCCYALLMKITSSVRWSSW
                     SVFALNCSFMLNALGLMLLPDSILLLLVFLLIFAAERLARRKQPADFIILGILLGLMG
                     LAKYTAILLVPPLLVFFLLKKRYDVLFSPYLFLAAAIALALVSPVFYWNATHDFISFK
                     YQGSHVFGSLATSFKNILESLAAQFGAYSPFLFIVAVYGFFKVIRDDNDYLRLSVLFG
                     GTILVFFLITSAYERTLPHWPSAFYALFIPIGTWRLLASGGKWRKNFLYVSVGISFFA
                     LMLAYAELGGKFIPFPDYKSPFRDIYGYPEIAAKADELLKQNPSSRPGAIAVTNWTMG
                     SRVMYYSLPYGHEVFVMDERRDQFDVWQKKSPLGYDLLFLNTEFEQIDVAGVLKCDQV
                     DRADRLDLTLNGSKVNRAEFVWCRNYQGVKP"
     gene            complement(73322..74551)
                     /locus_tag="PLQ77_00585"
     CDS             complement(73322..74551)
                     /locus_tag="PLQ77_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL09207.1"
                     /translation="MKVDTMRRIDYYIGVPLCFIGTLLHKTVRWFVKPGKASPRRVLF
                     MELSEMGSAVLVDPAMRKLRRETGADLYFAIFKKNKPSLDLLKTVPDENIYTFRENGL
                     VPLMWDALRFFFWTRRTKIDAVIDLELFSRVTALLTGFSGAPAKVGFHAFFNEGLYRG
                     SFLTHRVAYNPHIHIAANFVALVNALIAEKEEIPYSKKKVFAEDLVLAKAAVSAREKE
                     DVLTIIRECRAGFDPQKDKIILINPNASELLIQRRWPPAHYVRLMKMILEKCPKALIL
                     ITGDPSERQEADQLQKQVGDDRCVNFAGKVRFAQLPALYSVSEFMVTNDSGPGHFSAV
                     TDLPTFVFFGPETPALYGSLGITTPMYAGLACSPCVSASNHRKTACRDNVCLQVIQPE
                     QVFAAIAPHLERINAQG"
     gene            74743..75900
                     /gene="lptF"
                     /locus_tag="PLQ77_00590"
     CDS             74743..75900
                     /gene="lptF"
                     /locus_tag="PLQ77_00590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HPL09208.1"
                     /translation="MNKIINRYIFREIALPFVIILFVLTFVLLMGKILQIMDLMINKG
                     IRVLDIAHLIALIMPNFLLFTIPIALLVSILIAMGRLSADNEITVLKASGVSLLQLFY
                     PAAVASLLAFACAMVIGYFLVPQSNFATKRLLFELASQNAGIGIKEKVFNADFKGLLL
                     YADTIPADGASMENVIISDSRVLGEQNTILAKKAFLVADPKLMIVKLRLENGSIHTVG
                     KDLKNYRKVDFKSYDINLDLSSTMSSFTDEAKSSTEMTLSELLERMKKPDLAQAAMRE
                     LAIEVHKKFSIPLSCIFFGLLAMPLGITHHRSVKSRGFAVGLIIVAVYYLLRIGGEAL
                     VENGRMDPALGVWAPNMLFALLGVFLFYLAHREISVSQALIALFQGGKPRG"
     gene            75905..76984
                     /gene="lptG"
                     /locus_tag="PLQ77_00595"
     CDS             75905..76984
                     /gene="lptG"
                     /locus_tag="PLQ77_00595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HPL09209.1"
                     /translation="MKLIDQYILKEFLRFFLITFVSFIALFLIIDFFEKSRMFMSNHA
                     TATQMASYFLYSIPFVISMTLPVAVLLATLLTYSFLSKYSEITAMKANGISLYRISFP
                     VLAVAAAVSVFLFFFTELVTPAGVQKMEHIIKVEVQKQQALGFFKQDEIWYRSQNAIY
                     NFKMFDVEKNILQGVTINRLNPDFTMKTRIDAERAQWKNQSWVFYNLLVTTFQPNAPP
                     SLEWSEEKVIALPEGPNDFKIIQKDAEKMGYFELRRYVKKIQAEGYDVSRYAVDLQGK
                     IAFPVVCLIVVLIGMSFSIRSERSGGIMQSLALGLFLGFSYWIVHAFSMSLGRSGTLP
                     VFLAAWTANLLFGGLGAFLFYRVRT"
     gene            complement(77163..78587)
                     /gene="ahcY"
                     /locus_tag="PLQ77_00600"
     CDS             complement(77163..78587)
                     /gene="ahcY"
                     /locus_tag="PLQ77_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPL09210.1"
                     /translation="MAFQEIDKSLKYKVADLSQAAWGRKEMDLAENEMPGLMAVRKKY
                     GRQKPFRGLKIMGSLHMTIQTAMLIETLKELGADLRWASCNIFSTQDHAAAAIAKAGS
                     AAVFAWKGETLEEYWWCTEQALTWPDGSGPDMIVDDGGDATLFIHQGVKIEKDPKLLR
                     QKADNKEFAIILERLAQAWKKNPKRWQKVAAGIRGVSEETTTGVHRLYQMQAAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMIAGKMVVICGYGDVGKGCAQSMR
                     GFGARVVVTEIDPICALQAAMEGYEVRTLEEVASAADIFVTATGCCDVITGRHMEKMK
                     DEAIICNIGHFDSEIDMHYLETSRVCRKETIKPQVDKWTLKSGRSIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQTLAQIELATKKYNVGVYTLPKKLDEEVALLHLARLDAKISK
                     LTKKQADYLGVPVEGPFKPDYYRY"
     gene            complement(78692..78943)
                     /locus_tag="PLQ77_00605"
     CDS             complement(78692..78943)
                     /locus_tag="PLQ77_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HPL09211.1"
                     /translation="MAIHIIPVNKLSAKALKGVIEEFVSRNGTDYGAIEASLETSFQQ
                     VKNKLKNGSAVLIFDDETETTNIFPADDPVLKKLEAESD"
     gene            complement(78957..>80221)
                     /locus_tag="PLQ77_00610"
     CDS             complement(78957..>80221)
                     /locus_tag="PLQ77_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPL09212.1"
                     /translation="IHCRGGLGRTGTVAAQLLVELGMPHEKAIQAVRDARPDTIETRD
                     QEKYVYSCRPVNERLSVEHFSGCLLGGAVGDALGAAVEFDSIGAIRRQYGENGIENYA
                     PCYGRKGAITDDTQMTLFTAEGLLRAWCRKKQGIVPAEYPEMVHRSYLRWLSTQGVRS
                     AHEDFDLAQNGWLLTVADLHHRRAPGNTCLSALQSAKMGTPEKPINNSKGCGGVMRAA
                     PVGLFAAKAMQSEGNHGEVWKEIFDLGCRIAAITHGHPSGYLPAGCLATIICGIISGL
                     TIEESIKESVTVLKKAKGHEETLDAVTLAMALWKKKTAKPAPETIEKMGGGWVGEEAL
                     AISLYCALAAEGDFAKGVRLAVNHTGDSDSTGSITGNILGAMQGRAAIPAPWLEELEL
                     NAVIEEIAGDLFVLYDDGDHWQKKYPGY"
BASE COUNT        17466 a        23299 c        21403 g        18053 t
ORIGIN      
        1 cggaatcgct gatgctgaca tgctgggccg tcatgcagcc gtcgcccatc gtgacagacg
       61 tggccgccat aattctcgtc cccgggcaga tcaggcagca acggccgatt tcgatcttcc
      121 ctgcgctttt ccaggccggc cagacggtca gcctgacttt cttgtccggg gtggcaatga
      181 cattcgaatg atccccaagg gaaaccggtc cgccgaagat ttccacgtgc caaggcttca
      241 tgaataccag tccccggccg aaatactcga actgcggcgc cagaaaatat tgagcgtaca
      301 ttttctgaaa ccggttgtaa aagcgcttga cgtaataggg tcggtgatcc ttcagcaatg
      361 ctttcctctt ttcaaagtgc cgcggctgac aatgaacatc aaaaatcccg ccgccgggag
      421 tccgcggtac cgttttcgga aatggatggg gtttactccg gccaggagct gtgggcgaat
      481 attttcgagg ccggcgtcat gccgttgagc gcatcccagg ccaacaaaac cgcggcggcc
      541 gtccagccgg ttttttcctc cggccagatc accgtgtcgg gaaacgtcac gcccatccag
      601 taggaaccgt cgaaatagcg cttgtcgcca agccagctga agatggcccg cgcccgggtg
      661 tggtcttcca tggcggccag cgtcaggatg aattccgatg tttcggccat cgtcacccag
      721 ggacggtcgc tgacgcagcg cactccccag tcgggcacga caaacttgtc ccacaagtga
      781 tcgatgcggg ctttcgcctc gtttcccctg acggctccgc acaggatggg atagtaccag
      841 tccatcgaaa aacgggattt gatcatgttg aaaagatccg ggcggctgcg tatggtttct
      901 cccagccgct ctctggcctt ctgccaggcg ggctttttga tgtccagcag gtccgcgacg
      961 gccagcgcgc acttgaggct catgtaaatc gagctgcatc cggtcaaaag cgccatccgg
     1021 tcgatggccc cctcgcggtt tctggcccag tagatttctc catccggcgc ctgcatgttg
     1081 accgcgtatt gaatgccgcg tgataaagac ggccacatgg ttctgagaaa agtcctgtcg
     1141 cccgtaatca gatagtgatg gtaaacgcct acggcgatat aggcggcaaa atgggtttcc
     1201 ttcgttgaat tggtgaccac tccgtcgcgg atttccgacc accagcttcc gtccggcaac
     1261 tgggcggaaa ccagccaggc ataggcgcgc tccgcctcgg cggtccatcc ggcgacgctc
     1321 aggcccatcg cgctttcaat gtggtcccag ggatccgtct ttccgccccg cgaccagggg
     1381 atttcaccgt tttccttctg cagggaggcg atgaattcgg ccaccttttc cacatcaatg
     1441 ggtttgagct cttcgctcag cgatatattc aattccatac ggtcagccct ttttcgaata
     1501 aaacacgatg ctctttccga tgaccggatt caacgcttcc tccagaaccc gcaccgccct
     1561 tggcttgcag aacatgtccc aaaccagaaa ccgatgataa agcttcacaa aataattatt
     1621 gtcgttcttg atgccgacca ggcacttaag ccaccagtag ggcgcgtgca gcgcatgttt
     1681 gtaattgatc ttccagcact tcatcccctg caccgccagc atcttttgca gatttttctt
     1741 tttgtaaatt cggatgtgcc cgccttcgtc gctcgagtac gccggagata tcatccagca
     1801 gatgcgctcg gaaaaatagc gcggaacgct gacgaccagc gtgccccggg gcttcagcgc
     1861 gcggaccagc tccttcagcg cggcttcgtg ctccggaatg tgctccagca cctcggagca
     1921 gatcacgcag tcgaaagttt cattcgcaaa gggcattttc gtaatatcgg cggcctccac
     1981 ggaaacctcc cgggaaagcg cgtccggcat ctccgagagc gctttcgccg cctcggccac
     2041 atcggcgggg ttgcggtcga ttccgaatat tttcaggccc ggcagtctcg ccagcgcccg
     2101 cagatgtctg cccgtgccgc atccggcatc cagaaccgcg aatcccggct tcagattcag
     2161 ctcctgcaaa tcaatggtaa tcatggattg cctcgcgata aacctcaacc gattcccggg
     2221 cggccttttc ccagctgaac acggaatgaa cgcgcctaag cccctcccgc gcgtattttt
     2281 gcctttcttc ggaagagtcc agcagatgct cgattgcccg cgccagcgcc ggcgcatccg
     2341 cgggcggaac gatgatcccg gcgtccccca ccacttccgg aagggccccg ccggcggcgg
     2401 aaatcagcgg cacgccgcag gccatcgctt cggccgcggg gatgccgaat ccttcgtaca
     2461 gagacggcac gacggcgatg gtcgatttgg cgtagtaatc ggcaaattcc tcgttggcga
     2521 ttcgcccggt aaaatgcacg gtgccggaga gattcagacg ggccaccagg ttttcaatga
     2581 tgccgtcttt tttgggctgg ccgatcaccg tcagccggat cggccgcttt cggcggatct
     2641 catccaccgc ctccagcagg taccggagcc ccttcagggg cgtatccgcg ctgttggtca
     2701 caatcaggga attttccggc cgcggaccgt tttgcttggg ataaaagtat tcgttgttga
     2761 cgccgttgtg caccacgcgg aatttatttt catcaaggga gaattccttg gcgatgtcct
     2821 ttcgggtgaa ggccgacacg gtgatgatgt gggaaaagct tttggccacc ttaagctgca
     2881 tgttgatgaa cgagtaccag cggaacacgc ggaatttttg ccgcagcgtc ctggccgcct
     2941 tgtattcttc ctgccggtcc acggtgatcg gatggtgaat cgtggggatc gtcggcatga
     3001 ctttctgcgc cagcctgccg atgccgtagg acaggcactg gttgtcgtgg acaatgtcgt
     3061 aatcgccgcg gtgctttttc agataagcat aaacccgctc gccgaaggag aagggctcgg
     3121 gaaagccgcc cgcgcagacg cttgcaaact cgtacagatt cagcggccgg gccagatccc
     3181 caaagcgctc cggccggaac agatggtcgg gattgtaaag atccaggctt ggcattttaa
     3241 tcaggcggac gcggtcgtcg agctgcggat agggaggtcc ggagatgaca tccacaaagt
     3301 ggccttcatc ggccagcgcc ttgctcaaat gcttgatata cacgccctgc ccgccgcagg
     3361 tgggattgcc acgatacgtg agcagacaaa tcttcaacgg ccctgaattt gaattctttt
     3421 tcacgcacgg gccctttgaa cgatcttttg ggaatttttg aacatgatga cgatttcttt
     3481 ttcggataag cccgcgccgc gggcgatgtt tacggccatg gggcggcctg caaaatcacc
     3541 cggcgcgtga gcgtcgttgt tgagcaccag atccgccccg cattttccgg ccatggcggc
     3601 cacgtgtccg ttggtgtagc tgtgcccccg gcgggtggtg atttccagat aaacgccttt
     3661 tttcgcggcg agcttcgtct cttcgcgtgt aatcagcccc gggtgggcaa gaatatccac
     3721 tccggcgcgg atggcggcca gattggtccc gggaggaacc ggctcggtga gggtttcccc
     3781 gtggacgacc actatcttcg cccccagctt tcgggcttcg gccgcaagcg aagcgatgtc
     3841 ggagggatcg acatgcgtca gttccacgcc gggtatcacg aaaatatcgc ctttttccga
     3901 aagcttgccg caaacccgga ccattcgcgg aatcacgaaa tcgatattgg aatcatcgac
     3961 atggtccgta agcgcgatcg ccttgtaccc ggcgacaaag gcccttcggg ccgtctccgc
     4021 cgggatgagc gcaccgtcgc tgaaaagggt atgtgtatgc agatcaatca cgtgccgccg
     4081 tccatgagct tttgagcttt tgcaaaaact agtggtcact tagcaaattt gcgggcgtcc
     4141 gtcaataaag atgatgaaat cactgaaaat gaaacaatag ttttgattgt gttttgcggg
     4201 aaaatgacat agacagtccg gcaattgagc ggagaacatt ttcgaatgat ggcccggatc
     4261 gaacggggtc cccgcgacaa aagaacagaa ggagatcgtt tatgcacaag tttacactgg
     4321 acaagcctga caacctgatc gactggtggc aggaaagcga agcgaagttt tccgatcgaa
     4381 gacttttcgg caccaagaac aaaagcggcg tttatgaatg ggtcacgttt ggagacgtgg
     4441 gaacccgcat ccgcaatctc agggcgggtc tggccaagct ggacatcaac cggggagatg
     4501 tggtcggcat tatcgccaac aaccgcgtgg aatggcccat tgtggctttc gccgcctggg
     4561 gacgcatcgc ccgctttgtc cccatgtacg aagcggaact ggtgcagatc tggaaataca
     4621 tcattgccga cagccggatt aaagtcctgt ttgtttccaa accggaaatc tatgagaaaa
     4681 taaaggactt cccgaaggat atttccacgc tgaagcatat ttttgtgatc gacgccgaag
     4741 gcgataattc catggccgcg ctggaaaaga aaggcgccgc caagccggtc gccgcacagg
     4801 tcccaaaacc ggacgacatc gccgaactca tctacacgtc gggaactacc ggcaatccca
     4861 aaggtgttct tttgtcgcac ggcaatttta ccagcaactc tctggcgggc ctgaaaatgt
     4921 atcccgaact ggcggaaaac gaaggccggg acgtggtgac gctcgccatc ctgccctggg
     4981 cccattcttt cggtcagacc ggcgaactgt acgcggtcat ccgattgggc ggctccatcg
     5041 gcctggcgga aagccccgcg acgattgtcg atgacattgc caaggtgaaa cccacctttc
     5101 tggttgccgt gccccgcgtt ttcaaccgca tctacgacgg cctctgggcc aaaatgaaca
     5161 aggaaggcgg tctggccaaa aaacttttcg tcatgggcgt tgaggcgggg aagaaaaaac
     5221 gcgagctggc tgcgcggggt caatccgatt ttctgaccga actcaaattc aggatcgcgg
     5281 ataaaatcgt cttttcgaaa atccgcgaaa agctgggcgg ccgggtgaag ggcgccatga
     5341 ccggcagcgc cgcgatgaat cctgaaatcg gtcatttctt ctttgacatc ggcctgccgg
     5401 tttacgactg ctacgggctc acggaaacgt cgccggctgc aaccatgaac gcctcctatg
     5461 cctaccgcct ggggagcgtc ggccgggcca tcgagaaggt ccgcatcgtg atcgattctt
     5521 cggtggtgga ggaaggcgcc accgacggcg aaattatcgt ttacggcccc aatgtcatga
     5581 aaggctacca taaccggccg gaagatacgg ccgcggccat gacgccggac ggcggcttcc
     5641 gcaccggcga ccgcggccgc ctggacaagg acggctacct ctttatcacg ggacgcatca
     5701 aggagcagta caaactggaa aacgggaagt tctgcttccc cgtatccatc gaagaagaaa
     5761 tctgcctgtt gccgctggtt cagcaggcgg tcatttacgg atacaaccgc cccttcaaca
     5821 tttgcattgt cgttcccgac tttgacgttc tgaaaaacac cgcaaaagaa aaaggactgc
     5881 cgacggacat caacgagctg gtcgaaagac aggatatcgt ggatatgtac ggcgccgcga
     5941 tcacggctca tctcaaaaac aagttcggcg gctacgagat ccccaaaaag tttttgctga
     6001 ttcccgaacc gttcacgctg gaaaacggca cgctgaccca gaccatgaag ctcaaacgca
     6061 aagtagtgct ggataaattt caggatcgga ttgaagcgct gtaccggtag caggatccgt
     6121 gtcccgcagg aacaataaaa aaagccggct cctttttacg cgagccggct ttttttcaat
     6181 gatgacaaat gaaagttatt taaccgcgtc cttcaaaccc tttccggcct tgaataccgg
     6241 aaccttcttg gcggcgatct tgatggcttc gcctgtctgg gggtttctgc ctttgcgggc
     6301 ctttctcttg ctgacagaga aggtgccgaa tcccaccagg gttacgctgt cgcctttctt
     6361 gagcgccttc tgaattgccg ccaacgcagc gctgatcgcc gcatcagcct ctttcttcgt
     6421 gcttgtcact tttgccactt cttcgaccaa ttctgctctg ttcattgctc acccctttaa
     6481 gttttatttt gtaaaacgtt ggccaatgct taaccctaat tcaaaaaaaa atctactgtt
     6541 ttttgtaaaa ttcattcgat tattttcgtg tattttgagg cctgaagggc tttccggccg
     6601 ttttcccccc tgaagcgcat atccgctctt catattttcc atgttttgct gtaaagggaa
     6661 aacgaatcca aaggaatttg cctgtctgga tgacgacgga aacacgctgc gcatggcccg
     6721 caacgcacag gagccctggt tccggatcaa tccaggaatc acccggcatg ggtggcgcag
     6781 gaagtttgca ggaaaccata aaccttaagg agaagataca gatgagcgca caaccggtaa
     6841 aaatccccac aaccttaagc gcgtcggaat tctttctgaa gatcgttgaa atcgcgccgc
     6901 ggcttccgtc gatgatcttc aacctgatca aaggactcaa aagcatcggc ggcaaaacca
     6961 acaactcctg gggactggaa cttgaaaaga tcgccgcccg gttcccggac aatcccgcgg
     7021 tcaagtcccc cgacggaacc ctcacctacc gggagctgaa tgagcgggcc aaccgctttg
     7081 cccattacct gatctccaaa ggcgtttccc gcggcgatgt ggtgacgctt tgctttgaaa
     7141 accgtcccga actgctggcc gcctactgcg gctgttcaaa agtcggcgcc atcgcctcgc
     7201 tgatcaacac caatcagcgt ggagactctc tggtgcacag cttcaatctc aaccggggca
     7261 aagttgtcct gatcggcgag gaatgctgcg agtttttcat cgaggcgcgc tccggaatcg
     7321 atctttctca atcgacgctg ggaatggtcc gggacgcggg gcaattcaag accaaagacg
     7381 tccccgacat tacggatgag ctggcgcggc aaaacggcgc gaatccctcc accaccaaaa
     7441 gcgtcacact gaaagacacc atggcgtatg tttacacctc cggaaccacc gggggactgc
     7501 cgaaggcggc cgtcatcatc aacaagcgga ccctgtcggc catgttctgg ttcgggaaat
     7561 tcgtcatgcc cgtcaaaccc acggatacga tttatattcc gcttcccttt ttccacacca
     7621 acgccatcac cgtcggatgg ccgccggcgc tttataacgg cgcggccgta gccctgcgca
     7681 gaaaattcag cgccagcaaa ttcctggacg acgtccggcg gtttcaggcg acccacttca
     7741 tttacgtggg agaagtctgc cgctatctga tggccacgcc cgcaaaaccc gatgaccacc
     7801 aaacaacgct gaaatacatc tgcggcaacg ggcttcggcc ggatatctgg atgccgttca
     7861 aaaaacggtt cggcattgat aaaatatacg agttttacgg cgcggccgaa ggggtgggtg
     7921 ttttcacgaa cttcctgaac atcgactaca gcgtgggcac cagcgtgacg ccgtttgcca
     7981 tcgtggccta cgacaccgaa aacgaccggc ccgtcaaaga cgaaaaaggc ttcctgaaaa
     8041 aagtcggcaa gggcgaaacc gggcttctga tcatggaaat ctccgaaaaa acgcccttcg
     8101 ccgggtattc ggacaagaaa aagacggaag aaaaaatatt ccgagacgtt ttccgcaaag
     8161 gcgatctgtg gttcaacacc ggcgacatgc ttcgcgacat gggtttccgc cacgcgcagt
     8221 tcgccgaccg gctgggcgac accttccgct ggaaaggcga aaatgtggcc acgcaggaag
     8281 tcgaaaaagc gatcgacacc ttccccggcg tcaagcagtc tgcggtttac ggcatctcca
     8341 tgcccggcag cgacggaaag gccggaatgg ccgccgtcat tcgggatgaa aacgagccgt
     8401 tcgatctggc ccggctggca gcccacctga aggcgagcct gccgaaatac gccgtgccga
     8461 tcttcatccg tttcgttccg gattttgaat ggaccgccac gcacaaaatc aagaagacca
     8521 acctcaagaa tgagggctat gatccggcgc aggtcaggga ggaactgtac gcgcttctgc
     8581 cggggagcga aacctatgtg cccgtcagtg atgaaatcta tcgcaacgtg acggccggaa
     8641 cgtataaatt ttaacgttcg acgccgtctc cggcaaaggc ggcggccagg cgccagggcg
     8701 tgccgtgcag ttccacggtg cgccattcca ccgttttgaa aatcggcgct tcaccgactt
     8761 ttcggtaaaa acggtaaacg cccgatcctt cgcgggtatc ggccattctg cggcaaaggg
     8821 caatcagctc cggaaacggc tggtataacg gatcggtgaa gatgttgagt ccgatctgtt
     8881 tcgcatccac gtcataaata aaaaggccgt cggtctgcat caggaaaatg gaaaccggca
     8941 tgtaggcggc gatcggtccg atcatgctcg aaagcaggta ttccggcgca aacagagccg
     9001 agaccgatcc cgaaaattta cggtctttta aaaatacggg atgatgaatc acgaccgccc
     9061 gggggccttc cacggactgg aatgagccgc tcaatacagg cgtgtgggtt ttgaacagcc
     9121 ggaccatgtg cgactgtcgg ctgatgtcgg cgccttcgga cgaccggtgc gccggcggct
     9181 ccaccgccag catgattccc gaatcgctca ccgtctcaca gtcgatgata tagggatttg
     9241 ccctgtaaag cccctggaga atgccgcgcg caaaatcgcc ccgcaggtct ttgccggcca
     9301 gcacctttgc gccgtgccgc aaatcattgt ccaaccggtt cagcgtcgtc tgaatctcgg
     9361 attgcagacg ggacaaaaga tcccgcaatt cctgtcggga aagcgccgtc cggtgtccgc
     9421 tcgcttcggg aaccgccagc tcgcccgaca caatttttgc ccgcagcgcc tgaacctgct
     9481 gatgaatgct ttctggaatc agcttcttat tctggttatt gtaaacaaat ccgacgcccc
     9541 gttccttcag ccccagcgtt ttgagacctc ccgaaaagtt tccgtgagcg caggattgaa
     9601 cagcctcgaa caccgccccg tcgatatttt tcagcatgct cgtcaggaca cggcccggcg
     9661 cctgcgcgct ctggtcgata tccgctccga tcgccagacg attcatcttt cttgccgcct
     9721 gaaagaggcc cgctcccgtt tcacctgcgg catgataaat gacgtccgct ccgtttttat
     9781 acatgctcac cgcgatccgg taacctttcc gcggatccct gaaggcctgt ccggtgatgc
     9841 ctgcatagcg ggaataaatc cggatgcccg gacgaacggc ttttgcgccc gcctcatacc
     9901 ctgcctgaaa ttttcgaata atcgggctgt ccattccgcc gataaaaccg attttcccgg
     9961 accggctttt cagagccgcg atcgccccga ccagaaagga cccttcctct tccctaaacg
    10021 tcagccccgc aagattgggc ggcggcgaga tgaggtttcc tctctcatcg taattcgcgc
    10081 tgtagtccac cacgacgaat tttttatccg gatgccgccc ggccagatga tcgaattttt
    10141 ccgaaaaggc aaaaccgacg ccgatgatca tttccgcgtc ggaagccgcg gccccgctta
    10201 cggcataatc cagctcgagg tttcgtttat gctcgacata gacggccttc acgcccagct
    10261 ccgcaaccgc tttttccatg ccgctgtatg cggaatcgtt aaaaccgccg tctcccctgc
    10321 cgcccatgtc aaagatcagg accaccttca gcggcgatga atcctgtgct ttcgcgtcct
    10381 gaacaaccgc cggagagccc aacaggatca gccagcacaa aaaaagcacc catgcctttt
    10441 tcaatcttcc atctcctttt ccgttggctg caaagacggc ccctacttcc tcgtcttcac
    10501 caccgctttg acttcggaaa attccgcggc cagtcccgcg tcgtccgacg cggccttgat
    10561 cttgacgctc cttgttttca cgccgatgtc ttttgatctg gtttgaacgg cgaatatgct
    10621 tccctcatcc ttcagcttca aatcgcccag ctccgagacg ctggcataga cgatgatgtt
    10681 ttcctcgcca aagggcgggt tcacttccag ctcaaactga tccttgccgg aagggatttc
    10741 gtaaatcacg ccgccctgga agtaattgtc ccggcggtag ggattgggca ggagctgcag
    10801 gttgtttttc accgcgtccc ggtaaatcac acgcgcaaag aaaggcttgt tgcccttgag
    10861 ataaattctg atcttctctc ccgcggcata agacgttttg tccgtccaga ccttcacggt
    10921 caaaggcaga gacggatcat ccagcgcggc atccgcagcc tttgccccgg cctttttcat
    10981 cgccttttca tccggaatga cctccgcttt gagcttgatc cggtagcagt cgccctgccc
    11041 cgggtcttta taccagccct tttccagcgt ctcgagaact tcgacggtgg cgttggcata
    11101 agcggacacc aggtctttct gcagctcgaa gtctttcacc tccgtcgcgg atgaaacata
    11161 ggtcttgacc gattcgacgg cgtttcgctt tgcatccgcc agcgccgcgg tttccgtctg
    11221 cttgcgggac ttgtcgtccc ccatgcaggc ggagccttcg gcgatcttga tggtggattg
    11281 cgcggcataa accggcgccg ccaaaagaat gatcattgcc gcaagaaata aaattttacg
    11341 catggaaacc tccctcatcg tttatctgcc aagcaccagc ggaaaatcca ttccccggga
    11401 atccaccacg gggtactggg cttcctgttt atactttttt gtgatttccg gcaactgctc
    11461 ttccacgtaa accatcagct tgcggacggt gacgggttcg cccggacccg ccgccttgcc
    11521 gcccaagccc gcaagcagcg tataggtgaa aacgccgtgc cccagatctt tcacttcggc
    11581 ggcaaactgg tctttggtgg acgcggccac gacatgcacg cccgtggagc gcgatagctg
    11641 ggaaagcgct ttgcggtcct caaatccgcg gaaagcaatc agcacggcgc ccgatttgca
    11701 ggcatccacc aaaagcagga ttttttgcgc tttgatgttt ttgatatagc cggacagatc
    11761 atcggatgaa acacccttcg tccgcacatc cgcttcccgt tcgggatagg tcaattcatg
    11821 cgggatgaag taccatttat cgttcaggct ctcaccatgc cccgcgagat agatcagcac
    11881 tgcatcctgt ggattggtat tttgcagctc gttcaattga gagacgatat tctcccgggt
    11941 ggcctgctca ctgtaaattt cgcggatatt gactttcctg aacaatccgc cgccccgctg
    12001 actgaagaag ccggcaatgc tctttgcatc cggctcggcg tagttgaggt tgagcgcggg
    12061 attcttgtat ttgttgatgc cgaccgccag gacataaaga gacacttcct tggaaggggc
    12121 cttaagccgg accatcagtt cataaggatt ggattccgtc ctgtctctgc tgaatcccac
    12181 ggcccgaaac gtattcgcgc catcgaccag ggacacggaa tattgccgga ccgtttcccc
    12241 gccccggggg acgattttga cggcgcgttg atcgccgccg acaaccttgc cgttatgata
    12301 aagccgaatt tcgtcgatgc cgccgcccgt gtctctggcc gccacggtaa cggccagcgt
    12361 atccgtattc aatacgcttc ccgccgcggg agacgtgatc cggacctccg gcggtgaaag
    12421 gatgcctttt cggatgtcgg ccgtcgcttc cactttcttg ccctgcagca cggaggccac
    12481 atatacggga ttgaaaaact tctcgaagag attgtcgatg gaatagacat tcaatccgat
    12541 gcgaacattc aggtgttttg cgccgcccgg agaggcgttg aaatatcctt ccggggtgat
    12601 cacaatccat tcaccattgg tgaagctgac gaattgggcg atttctttgc cggacgcgat
    12661 atcccactgg cgcgccgtgc cgtccttgct tgcggaaaga atgtaatttc catcccgcga
    12721 gaaggccagg gatttaatct cgtccgtatg tcccttgaat gttttgacct cgccgccctg
    12781 acggagattc cataatttca cggtgccgat ccagtcgccg gaaacgacga actttccgtc
    12841 gccggaaagc gccagagaag tgatgaaacc gggatgaccc ggaaaggatc ttatttcccg
    12901 gcccgaggtc agatcccaga gtccgaggat gccctgattg ccgccttcgg ccgcaacgat
    12961 ctgttttgag tcggcggtaa aagccacctg gctaaagcct tccctttttt gattcgcccg
    13021 cagtctgatg gattgctttc ccgtggccgt attccagatg accaggactc cccgttcttc
    13081 atcatgagag tatgatgctg cagcaacaag gctcccatcc ggagaaaaag ccgcgtaccc
    13141 gtcagatgat gacagactca cgtgatcagg gaaatttacc agttcgatcg aacggcgact
    13201 ccggacatcg agcaggctga cttttcctcc gtctttctcc gccaaaagcg cgtacctgcc
    13261 gtcgggagaa aaagtaacgg cctgttgcgg gctgtcgtag gccgtcggat agcgatcggc
    13321 ggcgtcgctg atgatcgccc ggtctgttcg gctgtcgatc acctgatagc ccttgtcgat
    13381 gattttgaaa aagcgccgat tgccccattc gttttgccct ttcgcccacc agttgtcgaa
    13441 tggctcgatt gtccggcggc cggtcaaacg gccctggcgg cggtcaaaca ccgcgagctc
    13501 ctcatttccg gcgattttga cctgctcctg gtccgctgaa aagaaagcca gattgattcc
    13561 ctggacatcg ccgccgaagc ttctgatttt tctgtgcttc gcggcatccc agagggaaac
    13621 cttatcggtc gcggaaacgg cgatcaaccg gccgtccggt gaaaaacgca ccgcggcatc
    13681 gtcaagttta tgatcatccg tcatgccggc ttccacctgt cccgtaacca tatcccagag
    13741 aatcatgacc ggagggaagg aggaaacggc aacaaagcgc cgcccgtcgg gagaaaaggc
    13801 gatcgtgccg ttggttttaa cactgcctgc cggaaagccg cttagcgcct ttgcctgttt
    13861 tccgctgccg acattccaga aaaaaatatt tctgtcctcg ccgatggacg ctacaaactg
    13921 accgtcggga gagaacgcga ccttgctgac tccttcggtg tgtccggcaa agcggtgtat
    13981 ttcttttccc gactttacct cccagagtct gacggtgtca tcctttttac ccttgttcag
    14041 attgggcgtc tcgcttcccg atgccagata ttttccgtcc ggtgaaaagg cgatcgtgtg
    14101 aatgctggag gaatgccctt tgaagatcat caactcgcgg ccggtattga gatccaggag
    14161 ctgaatgttg cggaaagtaa gatccccgcc ggggaccttc tcgtcctggc cgcccaccac
    14221 ggcaagagac gtcccgtcgg gagaaaaagc agccgcaatc gcgaggagcg gcttcccctt
    14281 tttgccaagc ctcctgattt cccgaccggt ctttacattc cataacgcgg cgcccccctt
    14341 ggattccagg gaaagcaggg attggccgtc gggactgaag gcgacaaaat caaccatgtc
    14401 ccgggcgacg aatgtccgaa tctcacggcc cgtttccacg tcccacagct tgatgttttt
    14461 atcagagctt cccgaaacga gatacttccc atccggcgaa aagtcgatgg tgttgacatt
    14521 tgtggaatgc cccttctgca caaaaatatc cggcccctgc gaacttcccg ccgggacaag
    14581 cggtttggcg caaaaagctt cggttgtaaa aaccataatt atcaaaaaac aataaacaaa
    14641 aactaacttc ctggtcataa ataccctttc atacgcctgg agcaaggaat cttgccaacc
    14701 taaggtgtgg acttgccacc tttaggtggt tgcaactcct tggtctccct ccttccatcc
    14761 accatgcacc atacaccatc cactacttca ctttcccgat ggaaaacgcc tgtccgctga
    14821 tggaaacgac cggataccgg gtatgtttga aaatcttctc cgactgtgcc ggcatttcaa
    14881 cgcgcacata ggcggtgaga tccgtgctac ggatgctgcc ggttttatct ctgtctgccg
    14941 caccccgcaa gccttcggcg accaggtgga cgaacaggct gtgcccctga tagcccgcca
    15001 ccgcctccga aagaggcgcg gatgaagcca gaacggttaa cccgagcgtt ccgccgaata
    15061 tcttcagagc tgtctcttca ctcattgccc tcgtctggaa agccgcttgc gcatccgcac
    15121 tctgaatgcg gggattgcag gcatcaatca gaatcagttt tccggccgtc ggaatattgc
    15181 cgagagcctc catcagcttt cgcagggaga tggcgtccgt tttgagtttt tccgcactca
    15241 gcgaattcac attggatgtc agaagaaaat attccccatc ctcaacaacc gcatatgcgg
    15301 caatatacag gacaaacaga tcctccgcgc gcagggtctg atgtgtttga atctcccgaa
    15361 ggatcgcctc catcgttgtt tcttccggcc gggtcatggt cttgacggcc ggcttttcat
    15421 agattccttc cgatgccccc ttgagcgcgc ccgcaacaag ctccgcgctc gccgcggcaa
    15481 agggaagctt gagccccgga tctttgtatt caccgacgcc gatggccaaa acggcaagcg
    15541 acggcccggc cgccgcggca taggaggctg tcactacttc ccatgccgcc ttgttgctcc
    15601 gcatggtgtt gtctccgttc aatgctgcgg cgtggatgac atttttcccg cgggcaagct
    15661 tcaggggata ggccctgaac acggcccgct gcgacttttc cggctccgaa accgaacggg
    15721 aatccagaac cacggcggtc ccgttcagat aaagacggat gtccccgatg ccgccgccct
    15781 gctcgtcgag gttcagccgg acgtgaactt catccgcggc gacggaggcg ggcgtatcga
    15841 caatcctcac tttcggcggt tctttttcgt ccgtcaactt cggcaaatct ttcagggaat
    15901 ctccggccag cgcccttttg accacttcgg gacggtaaaa ggattcccgg tagttttcga
    15961 tgccgtaaac cgaatctccg acgcggacgt tgatatggcg gtctcctctg gacgaggcgt
    16021 tgtaatatcc ttcgggcgtc ataacaatcc atccgccgtc ggtgaaactg acaagctgca
    16081 caatctcttt gcctgcgggc agatcgtatt cgctgatgga aatcccgtgc cgaaggatgg
    16141 ccctgcgccg gtcggacgtc agcgatacgc tgaggacttc ctgttccact ccgatggaag
    16201 cgtttttcct gccggacgtc aggtcccaca ccgtcatttc gttctgatct ttgtccacag
    16261 cgaccatcat ttttttgtcg cgggaaacgt caaacggtcc gggcggcaca aaatcggcaa
    16321 atttcctcgt caggcggccg gtcgccgcat cgatcgtgta aaatcctcct tcgagatcgg
    16381 cacagaggat gagccttccg ttcaaggaat aataagattt tgccgcattc ttcccgcact
    16441 ccagaactct ctccaactgg ccggaggagg cgtcccagat catattccgg gaaagcagga
    16501 aacggccgtc gggggaaaaa gccacccagg gatagggaac gttgggttgg gcaaacgcca
    16561 gcaggctttt ccctgtcgcg gtttcccaga cgcccgatcc gcaggcaagg cgaaggccgt
    16621 cgggggagaa ggccagaatt ccgctcccat aatcgacgaa gtctccggcg gcaagctctt
    16681 cggcgtaaat gggcttgtac tccgtcttca cttcggacac cttccgaccg tcgttcatat
    16741 tccagattcg aataacgccg gagcgggcaa gcgaagccgc aaaacgacga tcgaaagaaa
    16801 ccgccgtggc cagaatcatt tccttctcct gagccggcag gctctttttc agtgtgccgg
    16861 cgccgatgtc ccagagctga acctcccctg ccacatctcc cgttacggcg acgcttccgt
    16921 ccggcgacaa cgccagcgca ctgatctcca tcgatgccct gccggtaaaa ggcgcgagcg
    16981 gcgcgccgct cgcccggtca aaggcctgca gggtattgcc ggacggcgcg acaatttttc
    17041 ttccgtcata ggtcagcagt ccgtgtccgg cggcaaagcg ccgaatctca agtccggtcc
    17101 gaatatccca caaaacgatg ttccggtcgt agcggctttt ggtcagaaca atgccttctt
    17161 ccgcgcaggc ggaaagggaa agaaaagcgc cgctctgccc gaagcggccc agaatgccgc
    17221 cgccggcaat gtccacgagc gtgctctttt tagcgctgtc gagatagagc ttttgtttct
    17281 tcggtccggc cgacggggca aatgcctgcg gcccccgttc ggtcagctcc cagtattcgt
    17341 tggccagaaa atatttcccg tcttcggaaa aatgaaaatc cgcgctcatg tattttttga
    17401 cttcacgccc ttccggcgtc aggactttga tcatccggtg gtccagcact ttatcgtcat
    17461 aggcgatcag cagatggcgg ctgtcgtccg caaagccgat ggatagggcg cggccgggaa
    17521 acgggaaggt tttttgaaca gcgccggaat ccaaatccca aagccggacc tcgctgcttc
    17581 cgtcaaggct taaaatcctt cggccgtccg gcgagattgc ggccctgact attttttccc
    17641 ggtggccggc aaacgtcctt aacagcgtgc cgcctttcgg attccacaac ttcagggttt
    17701 tgtcctcgcc ggccgaaaga gcgtatttgc cgtccggggt gaagagaacg gcggcaactc
    17761 tttccgtgtg cgcctgaatg gtttccagca gtttcccgga agttttatcc cacaggcgga
    17821 tttttccgtc cggcgtcccg ctcaaaacat gtcggccgtc gggcgacatc accagcacat
    17881 agccgctgtc ggcttggctt ttgagcgccc tcacctcttc accggttcgc gcgtcccaga
    17941 tccgtatgtc gccgtccgca tctccggata cggcaaaccg tccgtccggc gaaaaacggg
    18001 caagcgtaac cctcgcccga tgggcaaagg cccgaacttc ctttcccgct tcgatgtccc
    18061 agagcctcag ggtatggtcg cggcttccgg aaagaacata tttccgatcg ggtgaaaagg
    18121 cgagtgattc gatttccttc tgatgaccgg caaacacggc cgcggccttg cctgttttca
    18181 aatcccacac gcggatggtt ttatcccgcc cgcccgacgc ggcataggat tcgtccagag
    18241 agagggctac cgcatcgatc cggtctgtgt ggccgtccag cgtgaaaaga atattccccg
    18301 tggaaagctc ccacaggatc agttttttga accgggtatc cgacggatct tcggggacgg
    18361 cggaaatcat ccttttgccg tccgcggtca ccaggttttt gtgtccgaaa tcgtatcccg
    18421 aaaaatattt tttgacgggc gccccttcag gcagttgctc cgcgggcaga accttcccct
    18481 ccgagagctg cgcggcgtaa agccttgaag agacgatggc gcggacaaca gagccgtcaa
    18541 aagaaacggg gtcaatgatc atcggttctt tttcaccgct taaggaaaat cctccggtcc
    18601 gcgcgccgcc gtcaatgttc catattccca cgccgccttt ttcgtttacg gaaaaaaagg
    18661 atttgccgcc gggcagaaaa tgaacgtaca agattccttc accgtctgga aacgtgcgga
    18721 tcagccttcc gctttgggcg tcccacagct tgatgctgcc gtcgctgctt cccgaaagaa
    18781 tgaaccttcc gtcgggcgac cattccaccg catttacgga acccgaatgc cctgccggaa
    18841 tgaagatttc cgccttcttt tcggttccgc agggtgatgg ggggctatcc cctgccgata
    18901 caacaacagg gaaaagaatc cataaagaaa ggaagcaaaa ccatatcgtc gataagcgag
    18961 aaaatctcct gccggaaacc atagatgctc ccttcatgtt gaacgtgtgg acatcataag
    19021 ccaaaggtca tacgatgccc gcgccgcaaa gctgttgaat tttcccatgc tgacgatttt
    19081 tctgaaagta aggatcgtgt ctgctgtgaa taaaagcaaa aaaaaggaaa agaagcagaa
    19141 tcagcgcaaa aaccgtcagc agattccccc tcattcgccc caaggccggc atgaagccgg
    19201 acaaatcgcc ctgcccttcc ctgccgattt gcgcaaacgt gtagatcagg cccaaaacca
    19261 acggcggccc cagcagagga aacaccgacg ccgtcgccgc ggcatagaac cgccaggagt
    19321 tcagaggacg gccgccccgg cgccgcattt cggcgtaagc cccggccgtc atcaaaaccc
    19381 aggaagccgg gtaaatcatc ttgaaaaata catccgcgcc ggacgaggtc gtcatgccgg
    19441 caagcagcgc cgcggcatga cagatgcatc ccgcggtcag agcgtttctt ccaaaaacgg
    19501 tagtggcgat ttcgcgcgac ggcattatcg cctccgggct gaatcaggcg tttcttccag
    19561 caacatattg gcgcccctgt gtttggcaaa tactttcatg gcgtattgtc cggaggcctt
    19621 aaccggtcgt agtaggccat catggccgat tcgtacgcct cgccgaagtc gcccgttttc
    19681 tcgcctttct cgatggcgcc cctaatgtct ttcggcaata gggcgatatt ccgccgctgg
    19741 tctgcatccc aggccggaga agaaagatac ggagcgcaca gcatcacgcc ctttacgccg
    19801 aagggctttc gatccagcag gtaggagcag gccagcgcgc agccccagga aaatcccatc
    19861 aggcaaacgt cgtcaagctt taaggcggcc cgcacttccg ccaattcggc gacataattt
    19921 tccaccgaat aaaaattttt gtccggagcc cggtcgcttt tgccgcagcc caactggtcg
    19981 taaaaaaaca ccggacggtc tttccacagc ggctcgagac cgttatccat gctggaaaag
    20041 ccggggccgc cgtgaaggac gagaacgggc atcctgttcc cgccgccgcc gcgaacccca
    20101 taccagaggc tgccgttggc cgtgcggatc tttccttcgc ggctgctcat tcaatcttcc
    20161 ctcccctgga aaaaggatag gcgatgcttc cgaaaattgt cctgacgttc gcctcccctt
    20221 cgatgcgata gggaatgtcc ttgtcggcga tcacggcccg aaggctttcg ttccagtttt
    20281 tgaagccggc cgtgaccggc gcctgaatcc ttgtcgtgga caaggcgggc gccagaattt
    20341 ccttttccag gcgtccgctt ccgatttcac gctcgttcaa caaaagtttg tactgaagag
    20401 actgaatggt cagatcgaag cgattcggat tctgcacgtc cagttcgagg attagttgga
    20461 tttccgtgag ggagcggggg tgaagcgaaa cccggcgaag ctgcacggaa ggctcttcgg
    20521 tcaagcagcc cgcgcaggac gaaagcgcca gatacagcaa tccaaggggg atcagatatc
    20581 gttttttcat ggcgagacta tacgaaggat ggctttccct gtcaatggga ttgtccaata
    20641 ccggtccaat actggtagtt gacccggccg cgtcctgtcg atatactctt gcagaatttc
    20701 gacggttgaa acggctggga aagtttggcg cgcatggcaa aaatcaaatg gaattggatc
    20761 tggggcatcg gcatcacggt ggtgtttctc ggcctgctgg cgcatcgcgt ggagttcttt
    20821 cgggaaaaac cgcctgagtc tcccgagccg caaaccattg aaattgcccg cccacccgaa
    20881 tcctggatga atatttacca aaacaggaga aaaatcggcg taatccaccg caccttcacc
    20941 gcgctggaaa acggccgctt ccagacccgc gaaaacgtcg ccatgcagat caacaccatg
    21001 ggcgtcgtgc aggcgctgaa tctgtccacc gagtcggagc tctatcccga catgaccttt
    21061 tcctctttcc attttgagat caactccagt ctttttcgtt ttgccgcccg gggctacgca
    21121 ggccaggaca ggctcatcgt cttcgcggga ctgccccatg cccaaaagaa aaccgttctc
    21181 cccctgaagg accttcccca tatcagcgga aatatctatg aagcagcctt tcggggcggg
    21241 ctggaaaagg accaaacccg cagcttcagc atcttcgacc cgtcaacgct tgccctccgc
    21301 agcgtaaagg tcacgcgctt tgccgatgaa gtcatcccga tcatgggcaa gcgcattttg
    21361 acgcaaaaat tctgcgccga ctttatgggc gcccaaaact gcgcctggct ggccaaagac
    21421 ggtgaaatcc tgaaagaaac gggcatgctg ggtctgtcca tggaaaaggt ggatgcccaa
    21481 aaggcgcgcg aaggcatctc ggcggaaggc attgccgact tttcgcaaat ggcttccatc
    21541 gcctcaaacc ggattctgcc cgaaccttcc acattgacac agatcgtcat tcgggttggc
    21601 ggcgttcaca acctgcccct gatgctttcc ggcggcaggc agaaattttc ttccggcgtt
    21661 ttaacgatca ctcaagagga aacgcccgac acagcccagc cgaacaacgg cccgaagctc
    21721 tttcaacagt atctccagcc gtcgccgctg gttcagtccg acgccgcgga aattcaaaag
    21781 caggcggcca aaatcgtttc tccgaaagac agcccacccg ccaggctcag gaaaattgtg
    21841 cactgggttt accgcaacgt cgaaaagaag ccggtcctgt ccgtccccaa cgccctggaa
    21901 gttttgaaaa acaaggcggg tgactgcaat gagcacgcgg tgctcaccgc tgcgctgctt
    21961 cgggctgcag acgttcccgt tcaaatggaa accggtctgg tgtatttgaa aggccgtttt
    22021 tattaccacg cctggaacac ggcttacgtg ggccggtggg tgacagccga cgccgttttt
    22081 gaccagattc cggcggacgt cacgcatatt cgcctggcgc gcggcgaagg cgccgagcag
    22141 ctggacctgc tgggcgtcat gggcaaaata accctggagg ttctctctta caagaatgat
    22201 taaactgatc aacctgacca aggatttcaa aacgacgctg gccgtcaacc gcctgaacct
    22261 ggaggtggcc gccggagaaa tttacggctt catcggcccc aacggcgcgg gcaaaaccac
    22321 aaccatccgc atgatgggcg gcatcatcga accgaccgac gggaaaattc tcatcggcgg
    22381 gatcgacatg agccgggatc ccgttggggc caaaaagatc atcggttttg ttccggacag
    22441 gccgtttttg tacgaaaagc ttaccggcat ggagtttttg aaattcatcg cggatttgta
    22501 ccgcgtcgat ctccgggttt tctcccgcag agccccggaa ctgctggaac ggtttgctct
    22561 ctggcaatgg aaagacgaaa tcgtcgaggc gtattcccac ggcatgaagc agcgattgat
    22621 cattgccgcg gcgctgcttc acgacccgcg ggtgatggtc atcgacgaac cgatggtcgg
    22681 gctggacccg gaagcggtgc gaatggtcag ggatatcctg aaggggctgg cccagcaaaa
    22741 cgtgacgatt ttcgtgtcca cgcacacctt cagccttgcc gaggacttgt gcgaccggat
    22801 cggcgtgatt cacaagggaa cgcttctcgc gcagggaacg gtcgccgagc tgaatctggc
    22861 cgccaaaacc ggcgaagccc gcctggaaga agtctttctg acgctggtcc gggaattgta
    22921 agcctatgca aagcaccgta tggatattgc tgaaaccgag actgctgtcc gcccgaaacg
    22981 gactgaaacc gaatgaacaa aacgggaacc ggtcgcgact ggtccttttt tccctgatcg
    23041 gcgtcacgtt ctgggccggt gcctttgcca ttttttaccg cgtcctgaga tattttcaaa
    23101 acgttcagga tttcggcgac atcctggcca tgaagctttt gtccatgatg atcatcacct
    23161 tcttcaccct tttgctgttc agcagcatca tcaacagcct gtcgcacatg tatttaagcc
    23221 gggacctgtc cctgctgcat tccctgccgg tcgacgtcag ggatgcattc ctgtcgcgct
    23281 ggctgggcag cacgttcgat tcttcctgga tggtggtggc cttcagcctt cccgttttcc
    23341 tgtcctacgg cctcgtctat caggccggcc ttgcctttta ccccatcgcg ctttccgcaa
    23401 tcgtctgtct gtgcctgctg gcttccgcgg caagcagcct gctggtgctt cttggcgcgg
    23461 ccattcttcc cgccggaaag atccggactc ttttcgtcat catgggcgta ggcctggcgc
    23521 tgctccttat tttggttctg cgcatgattc gtccggagca gctggtcaat ccggacagct
    23581 tcgcctcggt tgttctgtac ctgaactcca tgcaggcgct ggactggccg tggcttccca
    23641 ccacctggat cacggatacg gttcgtctgg ccctgaaaag ggaatggggt ccgtcgcttt
    23701 tcagcctgac gctctcggca agctgcttct tttcattgat gttttttacc caaaccgccg
    23761 cgggcgccct ctattttcgg gggttttccc gcgcccagac caccgccaaa cggcttttta
    23821 cccccgtgaa attccacgga tacagttggg aaacgcttct gaattttctt tcccggccgg
    23881 cgagagcctt tgccgtaaag gaaatccgct cctttttccg ggattcgacg cagtggccgc
    23941 agctctttct gatctgcgcc ctgatcgtcg tctatctcta caacttctcc gttctgcccc
    24001 tggataaatc gcccatgaag acggtctatt tgcagaatgt gttttcattt ttgaacgtgg
    24061 ggctggccgc cttcgtgctt tccgccattg ccgcccgttt tgtttttccc gcggtgagca
    24121 tggaaggaga agcgttctgg atcattcaat ccgcccccgt ttcgatgaaa acattcctgt
    24181 ggatcaagtt ttttctctac tatattccgc ttttgatgct gtccgaaatt ctgattgccg
    24241 catccaacat tctgctgggc gtaactccgt tcatgatgtt tttgtccacc gtcaccatct
    24301 tctgcctggt cccggccatc gtcgggctgg ccgttggctt aggcgcccgc tacccgaact
    24361 tcaagtcgga aaatccggcc ttgtcggcca ccagtttcgg cggcgtcctt ttcatgctgc
    24421 tttctttcgg tctgatcgcc ctggtgatca tgctggaggc cggtccggtt tacagaatct
    24481 tcatgtccag aatgcacggc catgcctttt ccctgcttca gatcctctgg tttatcctgt
    24541 ccttttgtct tgcgctgttt ctttgcgtcc tcgcggtttt ctaccccatg aggcagggcg
    24601 aaaagaacct gctgaagaga tgatcctccg tccggcttcc ccgggggaaa aaggagcctc
    24661 aaaatcgcca gaagccgaaa attatcttga ttttacttgt tggatacgct aaacaaacgc
    24721 gcattctgca atgacccgtt tcatcagatc ttctgcggat ttgttgagat atcgaaagga
    24781 gtttccatgg caaagtacga acccaaacaa ctcagaaatc tggccgttat cggccacggc
    24841 ggcacaggca aaacctctct ttgtgaatcc cttttattta ttaccggcaa aaatgaacgt
    24901 ctgggacgcg ttgacgacaa tacgtccacc atggactttg agccggaaga gcagaaaaag
    24961 catgtttcca tctcatcggc ggtcaatttt gtcgaatggg ataagcataa aatcaatatc
    25021 gtggatacgc cgggtgactc caactttacc tttgatattc aatgttccct gagcatcgtg
    25081 gacaacgccg tcgtcgtaat tgactccgtg ggcggcgtcg aattccagac gcaaaaagtc
    25141 tgggaaatcg cgaatgggta ttctctcccg cgcctgattt tcgtcaaccg catggaccgc
    25201 gaacgggcgg atttcaataa agtgctggaa agcatcaaaa caaagctcaa gaaaaaagca
    25261 acccccgtct gtctgccgat cggcgcggaa gacaaatttc agggcatcgt ggacctgatc
    25321 gccatgaagg cttacacctt cgccgacaac aaaggcgcgg gaaaagccgt ggatattcca
    25381 gccgacctgg ccgatgaagc agccgagctt cgctcatcga tggtggaaga cattgccgaa
    25441 gccgatgacg aattgatgaa taaatacctg gaagccggcg aactttccgc tgaagagatc
    25501 atgtccggtt tgcgaaaagg cgtaattgcg ggaacccttc ttcccgtcat ttgcggctcc
    25561 gccgttaaag gcatcggcgt cactctgctt gcggatctgg ccgtcagctc tttcgcatcc
    25621 cccgtggacc gcgggccggt caaaggcaaa aaacctcaaa cggacagcgt cgaagaaagg
    25681 cagccctcgg aagacgctcc tttctcagcc atcgtattca aaaccatcgc cgacccttac
    25741 gcgggccggc tgacgctgtt ccggatctat tccggaaagc tcaattccga ctcggccgta
    25801 tataattcca cgaggaaaat cacggaaaaa ttcggtcata ttttctttct ggaaggcaaa
    25861 aaccagaaac aggcggagac gctcattccc ggcgacatcg cgggcgtggc caaactgaag
    25921 gaaaccgtca ccggcgacac gctgtgcgcc gagaaatatc ccattatctt tgagaaagtt
    25981 gccgttccgc cgccgatcat gtcgtttgcc gtggaaccga aatccagagg cgatgaagac
    26041 aagattgctt cgtcgattca ccgtttaacg gaggaagatc cgaccatcgt cttctcccgc
    26101 aacgtccaga ccaaagaaat gattctctcc ggcatgggcc aggtgcacat cgaagtcaac
    26161 attgaaaaga tgaagcggaa attcggcgtt gaagtcaatc tcaacacgcc gaaagtcccc
    26221 tatctggaga ccattaaggg aaaaaccaac gtccagggac gctataagaa gcaatccggc
    26281 ggacgcggcc agttcggcga ctgcacgatc gacatcgaac cgctgccgcg cggcggaggc
    26341 tatgaattcc tggacaaaat agtcggcggc gtcattcccg gccaataccg cccggccgta
    26401 gacaaaggca ttcaggaagc ggctgccaag ggcgtcatcg ccggctatcc ggtcgtggat
    26461 ttcagaatat ccctggtgtt cggctcttat cataccgtgg actcctcgga aatggccttc
    26521 aaaatcgccg gctccatggc gtttcagaaa ggcgttatgg aatgccagcc gattctgctg
    26581 gaaccgatcg tcaatattgc catcgaagtt cccgatgaat acatgggaga cgtcatcggc
    26641 gatctcaaca gccgccgcgg gcgcgttttg ggcatggaca ccaacggcac caatcaggtc
    26701 atcaaaggcc aggtgccgct tgcggaaatt ctgaaatacg cccccgatct tcgttccatg
    26761 accagcggac gcggcaactt tacctacacc gattcgcact atgaagaagt cccctcttac
    26821 atcgcggaca agatcatcgc cgaatcaaaa aaggaagcgg aagattaatg tttaatgccg
    26881 gcatcatcac cgtcagcgac aaaggctcgc agggaaagcg ggaggacaaa agcggccccg
    26941 tcatcgcaga aatgcttgcg ggcgccgcta ttcaaatcaa gcaaaccctc attatccccg
    27001 atgaagtcgc gcaaatcgcc aaagccatca ttcagtttgc ggacgttgaa aaactggatc
    27061 tgattctgac gaccggcggc acgggcgtca gcccgcgcga cctgacgccg gacgccacgc
    27121 ttcaggttct ggacaaggaa atcccgggaa tagcggaagc catgcgggcc gccagcatga
    27181 aggtgacgcc gcatgccatg atctcccgcg cggcggccgg catccgcggc cgatccctga
    27241 tcatcaacct ccccggcagt cccaaggggg cccgggaaaa cctttccgtc gtgatgcccg
    27301 cgctgaccca cgccatggaa aaaatccagg gcgacgacag ggattgcggg gtattgccgt
    27361 aaccaacccg aaaacctctt accgtttcaa ggaggatcgc caatggaacc aaacgtggga
    27421 cccatcgacc ggatcgctcg tctcgtcatc gccgttctat tcatcgtcgc cgtcatcgcg
    27481 gactggatcg gcggcattgc cggcgttgtc ctttgcgtca tcgccggaat gcttctctcc
    27541 agcgtggtgt cgggatactg cccgctatac gtaaaactgg gcttgaagaa atccttctga
    27601 ccaggatggg aagccgggaa aaccagacca gacgcggcgc cggtcccgaa gaggccctgc
    27661 gtgaaagagc cgggatgctc gaaagcatct taaacggcgt gttgctgctc atcacgtaca
    27721 ccgacaaaga tgaacgatac gcttttgtga accgggccta cgccgagtgg tatcacatca
    27781 agccggaaga gctgatcggg aaaagggttt ccgaagtttt aaaaccggac gtctacgcca
    27841 gggccttcga aaacattcag aaggcgctcg gcggcgagcg tgtcgcttac gaaaaccagg
    27901 tgaccgatcc ggaaggccgg gaaagaatcc tcagcgttca gtatgaaccg catttcgaag
    27961 gcgaagcggt aaaaggattc ttcacctcca tcgtggacat caccgggcag aagagcgcag
    28021 cccgggagct caaggagcgg gaacgggtgc tggctacgat gatcgccaac cttcccggat
    28081 tcgtttaccg ctgcgcaaac gaccacgact ggacgatgac gttcctgagc gagaagtgcg
    28141 ccgacgtcac cggatatacc gtctccgatt tcctccaaaa caaaatcaac tataacgaca
    28201 tcgttcatcc ggattatcgc gaatccttga gagaaaagtg gcaggaggcg atcctgcgga
    28261 aaggtttttt cgaggaagag tatccggtgg tcgccgcctc gggagaaacc agatgggtgt
    28321 gggagcgcgg acgcggcgtt tttgatccgg cgggagaatt gctctttctc gaaggcttca
    28381 ttggagacat cacggaccgc aagcgcgacg agaaggaaaa ggaagatctg cagacacagc
    28441 tgatccaggc gcaaaggatg gagtccattg gaagactcgc cggaggcatc gcccatgatt
    28501 tcaacaacat gctcgccgtc atcctggggc gcgcggacat ggccatgatg cggatggacc
    28561 ccgcccagcg tctttacagg gatttgcagg aaatccggaa agccgctgaa cgctcggcca
    28621 atctgacccg ccagcttttg gccttcgcgc gcaaacagac cgtggcgctg aaggtcctcg
    28681 atctgaacga aatggcggag ggaatgctga agatccttcg aaggctcatc ggcgaaaata
    28741 tcgatctcgt ctggcagccg ggcaggaatc tgcctccggt caaagtcgat ccggaccaga
    28801 tggaccagat cctggtgaat ctgtacgtca acgcaaaaga tgccattgga ggcgtaggca
    28861 ggatcatcat gaaaacgggg gccgcggcgc tcgacgaagc tttctgcgcc gtacacaggg
    28921 gctttgtccc cggagactac gtgacgcttt ccgtcagcga caacggctgc ggaatgaacc
    28981 aagaaacgct gaacaggatt tttgaacctt tcttcaccac caaggatacg ggccggggca
    29041 caggcctggg attgtccacc gtatacggcg tcgtcaagca gcacggcggc ttcatcaacg
    29101 cggagagtga accgggccgg ggctcgacct tcacggtcta tttgccccgc cacctgaaag
    29161 caggcattga aacggccgga aataaagaaa cggcatccgt cgccggaacg gaaaccgttc
    29221 tgctggtaga ggacgaacct gcgattctgg aaatggcccg aatgatgctc cagcgtctgg
    29281 gctacaccgt tctttcggcc ggaaaaccga cggaagcaat ggagctggcc cgcagccaca
    29341 ccggagagat tcatcttctg atgactgatg tgatcatgcc tgaaatgaac gggcgggacc
    29401 tggccctgag catttcattc ttccatccga aaatccggcg ccttttcatg tccgggtacc
    29461 cggccaacgt catcgcccgc cagggcgtcc tggacgaggg agtgcatttc attcagaagc
    29521 cgttttcgat gaaggatctg gcgatcaaac tgcgcgaggc catcgaaaac cggtaagaaa
    29581 atcccgcggc gccgcctgac tttttttcac aggccttccg ccccggacac caggatgccg
    29641 ttgtctttca aaaaccgcag ggcgttgcgc cggtgcgttc cgggccagaa aattttccgg
    29701 cattcgggac actgcgtgta ttcggcgcaa tgctcaaaaa catattccgg cacgaggtcc
    29761 cgaacattcc ggcgctccag ggaaatcatt ttttgattgc aggaaagaca aagcgtgtaa
    29821 aggtttcggg cctcgatttc cagcgacagc ttccccagca caaaagaaag ctgctttgca
    29881 acggccgtgt ccggcagaag cagcagacgc ccggaaaact gacgctccag gagatctctt
    29941 ctgcgggtaa gcaaaatccg gtcttcgccc gccgcctgcc tcatcaacgg ccggcccgct
    30001 ttctcctgaa cgcagaccgt gtcaaatccc agcagcctga gccaccgggc cagtcccgaa
    30061 agcgctgcgt cggcgagaaa tctggttgga gtattcatgc cgaaggaacg tgtgttttct
    30121 tgctgtcttc aatcatcggc ggggcttcgt gttccctcag ctccttgcgc agatcgcgaa
    30181 tcaccttgtt ctgcttggag atggtgcggt tgagccgaaa tctttccacc acgcccagaa
    30241 atcccgttat gacgacgccc accagaagcg cgacaaaaat gagcatgtag gccggaacca
    30301 ccagatcctg aaggtaatag tacttgatgg tcacgggcgc cgagttgaat tgtgaaaagg
    30361 tcacgataaa aaaagcgaac acaatggtga ggattaaata gaatatcttc atctcgtttt
    30421 aaacctcccg gcaatatttt tcaaacaggg ggatcagggc cgcggcggtg gcggccaggt
    30481 cctgcggaat aacgcggaca ttgccgacga cgctcatgaa attggtgtct ccctcccagc
    30541 gcggaatcac gtgaacatgg atatgctcgg cgattccggc gccggccgcc ttgcccaggt
    30601 tcatgccgat gttgaagccc tgggggttca tcgcctcctt cagaactttc acggagacgt
    30661 ccaccaggct gaaaatttcc gcccgctctt cagggttcag cgccgcaagg tcgcccagat
    30721 ggcgcagcgg aatgatcatc agatgaccgt tgacgtaagg gtaacggttc agcatgacaa
    30781 agcaggtctt cccttccagc accacataat catcgcagcg aagggaactt ttgcaaaaca
    30841 cgcatccttc cggtttttca cacatgagat actccattcg ccacggcgca cacattgttt
    30901 ccatctgtcc ggtacctgat cgcggtctat cgcgctattt gaggttgata atgctcccgt
    30961 tgatttcgaa gcccatttcc aaaattccga cgctccggtg tctggcaaac cgccggtcca
    31021 tcgccgaacc cggattgaaa aaataaatgc cgtcgatttt tttgttgacc gggtaatgcg
    31081 tgtggccgaa aacgatgcag tccaccttcc ccagtttttg ggccagcttt ccttccaggc
    31141 ccgcgggcga cccccagccg tgaatcattg cgacgcgaaa tccgccgatg tccatgatca
    31201 tttgctcggg cagcctctct ctgacctgcg ggccgtccat gttgccgcaa accgccttta
    31261 cgtccttttc gccgaaaagc tgaagaacgg atacatccac caggtccccc gcatgaaata
    31321 tcaggtcgac ctccgaaaaa ttttcatcga tgatccgctt caggttcggg tcgaacccat
    31381 gaacatgcgt atcggacaac acaccgattt tgactttatt ttccttcatc gccccgtgga
    31441 ttttcttatc ggtaagataa aaaaattaca agcgttattt cacgaagacc cttgcgcgga
    31501 atgcttttga attgactttg atcacaagga gggctagaat ttggaccatg gtcgatctgg
    31561 aagattcttc aagtaaaatc atggacaaag aaacggctca ccgccgcatt ttcgaactgc
    31621 gcaaaaccat cgaatatcac aacgcccgct actaccagca ggacgcgccg gaaatatccg
    31681 acgccgaata cgaccgtctg atgaaggagc tgcagaacct ggaggccctc tatccggacg
    31741 acgaactggc ctcgtccccg acgcagcgca tcggcgccgc cccgctttcc aaatttgccc
    31801 ccttcgctca ccctcagccc atgctcagtc tggccaacgc cttcggcgcc gatgaaatca
    31861 aagattttga ttcgcgcgtc cgccgcctgg cgaaatcgga gcgggtggat tatgtggcgg
    31921 agcccaaact ggacggcctg gccgtgaatc tgatttacga aaacggacgg ttccgccgcg
    31981 gcgcgacgcg cggcgacggc gccgtgggtg aagacgtcac gcagaacctc aagaccatcg
    32041 cctccctgcc gctgaagatg aagaccggcg ccttccgccc catcccggaa ttcatcgaag
    32101 tccgcggtga agtttacatg gaaaaggagc cgtttgaaaa actcaaccgg cgccgggtag
    32161 aggaaggcga tgctcctttt gccaatccgc gcaacgccgc cgccggctcg cttcgccagc
    32221 tggattcgaa gatcaccgcc cgccgtcctc tgaatatttt tctctacggc gtcgggtcgg
    32281 ccggcgatgc agcctttgcc agtcactggg aaatcctcga aacgcttgcc gcatgggggt
    32341 ttccggtcaa tccgctcatc cggcccatgc cggacatcaa cgcgtgcatc gaatattatg
    32401 aacagaccgg ctcgatccgc tccagcctcc cctacgacat cgacggcgtc gtgctgaagg
    32461 tcaaccgcct ggagattcaa aaccttctgg gcagcgtatc gcgctccccc cgctgggcgc
    32521 tcgcctgcaa atttccggcg caacaggcag ccacggttat ccaggacatt atcgtccagg
    32581 tgggaagaac cggcacgctg acgcccgtcg ccgtgatgga gccggtcaat gtcggcggtg
    32641 ttctggtcag ccgggccacg ctgcacaatg aggatgaaat cctgaaaaag gacatccgcg
    32701 tcggcgacgc ggttctgatt cagcgcgcgg gagacgtgat cccggaagtc gtcagcgtca
    32761 ttctgtccaa aagaacgggg cgggaaaagg aattccgcat gccgcgcgcc tgtccggagt
    32821 gcggttcgga aatcgtccgt caggccggtg aagccgccca ccgctgcgtc aatctgtcct
    32881 gtcccgcgca gttgaaagaa catatccggc atttcgcctc gcgcggcgcg atggatattg
    32941 aaggcctggg agaaaaattg tccgcgcagc tttttgacgc gaagatgatc gccgatccgg
    33001 cggacctgta tttcttgacg aaggagaagc tgctggggct ggatcgtcag gcggaaaaat
    33061 ccgcggagaa tctcctcgaa gccgtagaac gctccaagac ccccgcgctg gacaagttta
    33121 tcttcgcgct cggcatacgc cacgtcggcg aacgcaccgc caaacttctg gccggacatt
    33181 tcggcagcct ggaggcactg atgagcgccg gttgcgaaga attgacggcc atccgcgaaa
    33241 tcggcccgga aatcgcccag agcatactgg aatttttcgc ggagccaaaa aacaggatcg
    33301 ttttggaaaa gtttcagcgg gccggcctgt ccccccgaac cccggccccc acggcccagg
    33361 cgagtctgca gggcaaatcg tttgttttta caggctccat ggaaagcatg ggaagaaata
    33421 aggccaaggc cgccgttgag tccttgggcg gcatcgtcca ctcctccgtc acgggcaaaa
    33481 cctcgtatgt ggtcgcgggg agcgatccgg gatcaaaact tgaaaaggcc agaaagcaaa
    33541 gcattcccgt catcggcgaa gaagaatttt taaccatgat cggcaggcaa atccaccggc
    33601 aggcgtgaaa gattgttcat cctttccggg ccgccgcccc tcagaatggt ggttatgaaa
    33661 agaatgcatg ttttgatttc agggcgtgtg cagagggttt tctttcgtgc ggaaacgctg
    33721 cgaaccgcgc ttagcctcgg ccttgcggga tgggtgcgca acatggaaga cggacgcgtc
    33781 gaagcccttt ttgagggaga agacgaaagc gtggaccgaa tgctggcgtg gtgccgtcag
    33841 ggaccgttgc acgcccgcgt ggaccgggtg gacatgacgg aggaaccctg tcgaggcgat
    33901 cttcaaggct tccgtatcaa agatttttaa cccgcgaccg gttgcagaaa actgaacctg
    33961 aaaagacttg acgaagcaaa ctccggttct atatagcagt cgcaggacac gttcctgacc
    34021 gcccttggac gggcaacccg ctttgcacaa aaagaacagg aggaaatttt catgtcgttg
    34081 gcagatcgaa acaaccctta cagcttcaat gaattcctgg cctggcgcca gggtgcggat
    34141 tactacgccg atgatccttt cattcaaaaa gtcgtgcgcc actataccgg cgatatccgg
    34201 cgcgaggtgg atgccgaagc gcgcgccgtc tccgccaaag cctctttccg ctggcgcgac
    34261 atggccgagc ggatcgcacg gccggaggag cgcccctata ttctgcacta cgacggccac
    34321 cacaaccgca tcgaccggat cgtgcggccc cgcgatacgg aaatcatgga aaaagaaatt
    34381 ttttccgaag cgttctttgc ggagaaaaca cctccctggg tcaaactgat caagatgtat
    34441 atcatttacc agaacggcga ggcctgcatc ggctgccccg tcacctgcac ggaaggcctc
    34501 gtcgaactgc tcaacaagta cgccgattct ccggaaacaa aagaaatcct tcggcactgc
    34561 aaggaaggca tcgacggcga ctttgccatc ggcgcgcagt atctgtccga aatccagggc
    34621 ggatcggacg tgccgtccaa tgttctggaa gccgtgaggg aaaacggcgt ctggaaactc
    34681 tatggaacaa aattcttctg ctcggccacg catgcggatt atgctgtcgt gacggccaaa
    34741 ccggcaggct cggaaaaggt ggggctgttt gtcgtgccgt cgtggctcgc gggcagcaag
    34801 gaaaaggaaa tccgcaacgg ctgcaccatc gaccggatca agtggaaaat gggcaccagc
    34861 gaactgacga cggccgaact caccttcaac ggcgcggtgg cttaccccgt cgggccgctg
    34921 gacaagggag tggccaacgt ggtgggcatc gtgctcacct attcgcgcct gacggtgggg
    34981 ctttccgccg cggcttacat gacgcgggcc gtgcgggagg cggaaaaata cgtctccttc
    35041 cgcgacgcgt tcggccttcg gctgaatcaa tttcctctgg tcgccgtcca gctcgacaga
    35101 atccggaaaa ccacccgccg gaccaccgcc ggcgccttca agctctaccg cgatttcctg
    35161 aagctggaag gcggcctcaa gggcagccgg ggcgacgacg aaccgcttcc cgcgcgccgt
    35221 caccggttca atatccgcga actgatcatg ctgcagaaga tcgccgcctc ctgggactgc
    35281 accgatgtca tccgtgaagc catgtcgctt ttcggcggcc acggcgtgat ggaggatttt
    35341 tcgtgcctgc cgcgcctgta tcgtgactcc gccatcaacg aactgtggga aggcccccgc
    35401 aacgttcttt tgacgcaaat ccaccgcgac ctgcagaagg cagccggctg gtacaaaccg
    35461 gaggaattcg tcgctgaaat tcttcaggga ctggacaagg cgcaggttca ggagatggcc
    35521 gaagaaatca aagcgctggt cgcgcatccg cacctctttg gaatggatga ggccacgatc
    35581 gatgtctgcc gccgatggga cgccttctgc cacagattct cccacgccta tcaggacctg
    35641 gccgtgaagg aaatggactc cccgctcaag gggtaagctc agcagggtta ataaagtttt
    35701 tatgccgctg gtccggtttt gccggaccgg ttcgttttaa aaaactgttg ccttcgggca
    35761 gcagtttttt acttttgatt tgcatccccc ggcaaatcgg gttaaatgtt ttaccgtttc
    35821 ttcgcaacgg atcgacagca agcgggacag acaaaaggaa atgatgtttt tattgaaaca
    35881 ggtggttcat cggctgcgga gtatttttct ggaaaacatc gcgcggtggc tggtgttcgg
    35941 gcttttggtc ggcatcgttt cgggactcgg ggcgtcggcc ttttattacc tgctccaggc
    36001 cggccgccac ttctgtttta acctgctggc ggggtatgcc cttccggcgc ctgcgggtga
    36061 acagctcttc gaagccgcag gcacgcttca attccatcgc ctggtttttt tccttttgcc
    36121 cgccctggga ggtctgatca gcggtttgct cgtctatcat ttcgcgccgg aagctgaagg
    36181 ccacggtaca gacgcaatga ttgacgcatt tcacaacaag aaagggcgca tccggacgcg
    36241 tgtacccctc atcaagggac tggcttccat tatcacgctg tccagcggcg gcagcgcggg
    36301 aagagaagga ccgattgccc agatcggcgc cggcatcggt tcggccatcg cgcgtctttt
    36361 aagactaagc gaccgcgagc ggcgcatttt gcttctggcg ggctgcggag gaggtctttc
    36421 ggcgattttt cgcgctcctc tcggcggcgc actcaccgcc attgaaatcc tctacaagga
    36481 agacatggaa acggaagccc tcgtgcccac ggtggtgagt tcgattaccg cctatatcat
    36541 ctttacctat tttttcggca acgcgccgat ttttttcttt ccggaataca cgttttccaa
    36601 tccccgcgag ctgatttttt atatccttct gggtctgatt tgtgtgccgg ccgggatttt
    36661 tttcatcaag gtattttacg cggtacgcga gcgcattttt cagcctctga agatccccct
    36721 ctatgtcaag ccggcgctgg gcggcctggc ggtgggctgc atcggccttt ttttcccgca
    36781 ggtttatggt gacggctggg ggtggataca actggccatc ctgggcaaac tcggcatcgg
    36841 aatcatgatg gcgatcgcgt tggccaaaat catcgccacc agttttacga tatcgtcggg
    36901 cggttccggc ggtgtcttcg gcccgacact gtttatcggg ggaatgatcg gcggggctgt
    36961 ggggtattcc gcccattatt tttatccaaa tatcgtggcg cagccggaag cctttgtggt
    37021 tgtgggcatg gcgtcgttct ttgcgggggt ggccagcgcg cccatcggaa cacttttgat
    37081 gtgttcggag atgacccggg gatatggcct tatcgcgcct ctgatgctgg tgtccatcat
    37141 tgccattctt tttacacgac agcattccat ctatgcaagg caggttcaaa gcaagcgcga
    37201 gtcgcccgcg cacacggcgg atttcacggt gaatcttttt gccgaaaccc gggtgagaga
    37261 cttttacaag ccggaaaaag ttacgccggt gcgcaaaacc acgtcctacg gccagctcaa
    37321 gaaaatcttt tccgcttccc gcatcgaatg ctttcccgtt tatgacgaga acgacgcgct
    37381 gatcggctcc gtcaactggg atcatgcgcg aacgattgtc tttgaggaag gcctggagga
    37441 tctgatcatc gccgaagata tgatgagtcc cgttcgaacg gtgacgccgg aggataattt
    37501 ttacgacgcg ttcatgaagt tcatggaaac gaacgctgag gaactcctgg tggtgagccc
    37561 cgacgacggc aacattgtgc tgggcgtgct tcgccacgac gatttgattg ccgcttattc
    37621 cgcggaactc aaccggcgga aaaacagcgg gtgagcgaag agcgttcttt ttctactcaa
    37681 tgcccagctt ttcgatgcgg tattttaagg aatcgctggt gacgcccagc agatccgccg
    37741 ctttggtttt gatgcccctg gtcttctgca gcgccttttc gatcgcccgt ttctcaattc
    37801 ggttcatctc ttcgttcaga tcgattcccc cgtcgggaat ggacaaatcg atcgagactc
    37861 ccggcgcgtc cgcgtccgtc gtcagcacca gattttcggg caggatgata ttggaggtct
    37921 ccatcgccac gccccgttcg atgatgtttt ccagttcgcg gatatttccg ggaaagggat
    37981 attccatcag cagctccatg gcgtaagagg aaatcgtccg cacttccttg ccgaaatcgc
    38041 gcgcgtattt ttcaatgaaa tgccgggtca gaagcggaat gtcctccttc ctttcgcgaa
    38101 gcggcggcat gtgaatcgga atgacgttga ggcgaaaata caaatcttcg cggaaagatc
    38161 cacgggcaac cttctccttc agattctgat tggtggcgga gatgatgcgc acgtccaccg
    38221 tgatgtcctt gtcccccccg atgcggcgaa aggtcttctg ctgcacgacc ctcagaagct
    38281 tcacctgcag aacgggcgga agctccccga tctcgtcgag aaaaacggtt ccgccccggg
    38341 ccagttcaaa caggcccgcc cggtcgccgt aagcgccggt gaaggacccc ttcatgtagc
    38401 cgaacagctc gctttccagc aggttttccg gtatcccgcc gctgttgatg gccatgaacg
    38461 gcatcggggc gcgggaggag ttttcgtgaa tggcgcgggc gaccagttcc ttgcccgtcc
    38521 cgctttcccc cagaatcagg atattggcgg gcgtatcggc caccttgcga atggtcgaaa
    38581 aaaccttggc catctcccgg ctggccccga tcatgccgca aaacgaggtt tccgccggcg
    38641 gatttttttt cggcgtctgg gcctcgcccg gcgatccctg ctgcaacagg gcctgccgga
    38701 cgatcttctt cagctcgtcg atgttgccgc ttttttccac gtagtcatac gcgccttcct
    38761 tcatggcatt gacggccgtc tcgccggaag cgtaggcggt gatgaggatc acaacggttt
    38821 ccggccgctg ctccttgatg gtcttcagaa attcgatgcc gtcgatcttc ggcatcttca
    38881 ggtcggtgat caccaggtca aaggcggttt tgcggcacag gctgatcgcc ttttcgggct
    38941 tgccggcgca ggtgacgtca tagccttcct tggtcagcat aatttccatg aactctctca
    39001 tcccctgatc atcatccaaa accagaattt tcgccatgct tgcgctcctt tgatcttatc
    39061 ggcagggcag ccataccgtg attgcggttc cctcattgac cctgcttcgt atcgtgatcc
    39121 tgccgccgta gccgtcaacg atgcggctga caatcgccag acccagcccc gtgccccgct
    39181 ccttgttggt gaagaacggt tccaaaacct ttttcatgtc tttttcttca atgccgcatc
    39241 cgtcgtccgt taccttgatt tcggcatagt cccttttgtc ttccaggcgc acggtccgga
    39301 tttcaagatc gatcctgccc ttcccttcca tggactgcag ggcgttggtc agcatgtttt
    39361 gcaggatctg acgaacctgc tcttcgttgg ccagcgcctg agggtctccg gaaattcttt
    39421 tctccagctg aatgccttcc ctccagtcgg cggacaattt catgctatcc accacttctt
    39481 cggcaacatc attcacccgg acgaactgct tggcttccgg cacgggacgg gccagcagaa
    39541 gaaaatccct cacgaaactt tccaggcggt ccttgcccct cagaatgatc tgcatcagcc
    39601 tcttgttcgt ctcgtccagg ttcggggtct gcgccatcag ctggatcgag ccggtaatcg
    39661 acgccagagg atttctcatt tcgtgggcga ggccggcggc catttcgccg atcagcgcga
    39721 gccttctgct tttctcgaga ttttcctcca tgcgccgaat ctccgtcatg tcctgaaaaa
    39781 tcagaatatg gccgatctgc ccgctgttct tgtctttcag gggggaaagc gcgtatccca
    39841 gatagatcgg cacacccttt tggctgttga tttttatttc acccttgggc ttcatctgcg
    39901 ggccgtcgcc gttggaaaac agaggggcaa aggcgggaaa tacatcctcg atctgccggt
    39961 tctccacgga tcgaagcgga tggccggtta ttttttcggc ggaccggttg aatgttttga
    40021 tccggccctg cagcgtggtc gtcagaatgc cggtatccac cgattcgatg atgctgcgaa
    40081 aaagaaggtc caactggtca aaagccgatt ctttttccgc aagcagggac cgggcctttt
    40141 tttcctgctc gactacgaag ctggccagaa acgcgaggat gtaaaacgac agaatgtgga
    40201 cggcgatccg aatgaaaaca tcccccgcct ccagcgtgta atcccggccg gtcgcgtaaa
    40261 aagtggggat gacgccgaaa tattcaagat ccagcaccag cccgtaggcg atgctgcaga
    40321 ccgacgcgaa gatcagggcg ccccggcgcc ccagaaaaat ggcggcgtaa atgatgacca
    40381 gtgtgtacag aagcgagtag ttgctgccga tgctgccggt caggtacacc agaaacgaaa
    40441 ccagcacgat atccgcggca agctggaagt agatgttgat ctgaaaatac tttttttttc
    40501 ttaaaagcag tgtataaacg acggacagga tgtatatcgc gacgatgacg gcgtaaatgg
    40561 cgtgcagcat atcccgggag atgaaaatga tcgatttgac ggtgcccaga aacagggtca
    40621 ccagcagaaa agcggagagg atgaccaccc tgcttacgat cagcagcttg agccttcgcc
    40681 aggttctctc gtcatattgc atcggttgcc gcataaactg ttccgtggtt cttccgcgtg
    40741 agtattttga gctgctttta taatattttg cgttttttag ccaatcaatt gaacgcttcc
    40801 cgctgagagg tcctgttccc catgcgcaga cctttatggt acccgctggc cgccctgatg
    40861 gccggcatct ggatcggcga gggcatctct ccggcccccg ccgttttgcc ggtctgcatg
    40921 ctgccggtcc tgacgctcct gctggtgagc gtcagtcgcc gctggaatag agcagccttt
    40981 cttctgctgc tggccctgat gctgacggcc ggaatgctga atatccaacg gcagcccttt
    41041 cttgccggtc aggaccggca tattctccat tcggccggtc agggaaaaac gactctggaa
    41101 ggcatcgttc tttccaccga acaggcgctt cccgagagaa acacgctgat cgtggattgc
    41161 cggagagtcc ttcgcgacag caccgccgta cccgcaacgg ggaaaattcg tctggccata
    41221 ccgccggacc tttcgtttca atacggtgat ttcatccgtg ttcatacaaa aatcagggaa
    41281 attcagagtt ttcacaatcc cggaagcttc gattacgagc gggtccagaa ccgccgcggc
    41341 atttatgttg ccggatatgt ctccgaccgg gcaggcattg ttctgatccg cccgggcgcc
    41401 gccggcggcc tcaggctgaa gctggagcgc ttccgtctgc gtctgaagcg cctgattgaa
    41461 gcccgcgccc cttccccgca gagggaaatt ctgcaggcgc tgacgatcgg caatcaaaaa
    41521 tccattccgc agaatgtgcg cgacgatttc gcgaaaaccg gaacctccca tattctgtcc
    41581 atttccggcc tgcatgtcgg catcgtcgcg gcatcggctt ttttcctgat ccttatcgcc
    41641 cttaaatcct cggaatatct gatgctgaaa ttcaacatca tcaaggcggc gacgggagca
    41701 gccatggttc cggtcctgat ctacgcgctg gtcgccggaa tgggaacccc cgtccttcgc
    41761 tccgcgctca tgacgctggc ctttctggcc gcgcttctga tcgggcggtc caaggacctc
    41821 tacaacatcc ttgccggcgc ggcgctgctc atcctgatcg ttctgcccga agcgcttttc
    41881 gagatttctt ttcaactttc tttcagcgcg gttttcgcaa ttctttacgc ggttgacaag
    41941 ctgggcaacc ggcgggcgcc cctcccctca tcgacgcccc gctggatttc ggcgggcatc
    42001 ggccggctgt acctcttcct tctggtctcc gttgcggcca cgctgggaac cctgccgatc
    42061 atcgttttct acttcaaccg cgtttcggcc gtgacgctca tcgccaatct ggttgcggtt
    42121 cccctgctgg gcatcctggc gctcattccc gccatgctgt tcattctgac gtcgctcatc
    42181 tcggaggcgc ttgccggttt tttcatatcc gtctcgtcct ttttcaccgg catcgccgtc
    42241 aggatcatcg aagaactggc gtcgctgccc tggtcctcct tcagcttcgt aaaacccaac
    42301 gtcatggaaa tagcgctttt ttatcttttt ctctacttcc tggtccaggc cctgtcgtcc
    42361 ggcagcggga aggaccattc gacgcgccgg ccgatcctga tgcgaaccgg ccttatgatc
    42421 tgcgttgcac tgattcttgc cgatgccgct ttccttgtcc tgaaggaccg ttattccacc
    42481 catctgaaaa tcacggcgat cgatgtcggc cagggctcat cgacgcttct tcagcttccc
    42541 cggggcgtca acatgctcat tgacggcgga ggctttcacg acagctcgtt cgacatgggc
    42601 cgggccgtga tcgccccttt cctttacgcc aaaaggatac gaaaagtcga tatcgccgtg
    42661 ctgacgcatc ctcaccccga tcaccttcag ggtctgattt atattctcaa caactttgac
    42721 gtccgggaag tgtggtgcac cggcctcaca gccgacgatg atctttaccg gctgtggaaa
    42781 aacaccatct ccggacgcgg cgtaaaggtc agaaacctgt cggcgcaatc gccgcccgtg
    42841 aatctttccg gcgtaaactt ccggttcctg tggcccccgc ttcctccgga cccggtcggc
    42901 cgagccctgt cctatgagga taccaatgac gcatccctgg tcatgaaaat cacctacggc
    42961 acccggcgtt ttctcatcac gggcgacatc tccgcgtccg ttgaggcgcg cctgatcgca
    43021 tccggggaag acctcaaaag cgacgtcctt ttcgtgccgc atcacggttc cgccacatcc
    43081 agttcgcctc gctttgtccg cgccgtgtcc tgccgctggg ccgtcgtcag cgcggggaaa
    43141 aacaacgtct ttcgccaccc ccacaccgcc gttctggagc gctaccggtc cgccggcgcc
    43201 gggatcttcc gcatggaccg ggacggcgcc gtgtccatcg attccgacgg gcgggccctt
    43261 tccatcaccc cgtggatcaa aaggccggag cctgaacccg ccggccgccg ggccctgaat
    43321 atttcattgc caatgcccgg ttgatatgct agaagcatct gatttatttt ggaatataac
    43381 tatggaaaaa attacggccg ttaaaggcat aaaagacgtc ctccccggcg attcacccgt
    43441 ctggcaccgg ttggagcttg cggcccgccg ggttttcgaa tgcttcggct tttgcgaaat
    43501 ccgcgttccg attatggaaa aaacgtcgct gtttcagcga agcatcgggg aggcaaccga
    43561 cattgtcgaa aaggaaatgt acacgtttcg cgaccgcgac gacgaacttt tgacgctgcg
    43621 cccggaagcc acggcatccg tcattcgggc ctacatcgag cataatctct ctgcctccga
    43681 tcccgtcacc cggctttata ccatgggccc catgttccgc cgcgagcgtc cgcagaaggg
    43741 gcgattccgc cagtttcacc agattgacgc ggagctcttc ggggacgaca gccccgccgc
    43801 cgacgcggaa gtcatcttca tgctgatgca cttcctgcaa accctgggca tcggaaaact
    43861 gtctctggaa atcaattccc tgggctgcaa agcctgccgt ccggtctttt ccggggcggt
    43921 ggtcgattat ctgaaaggcc gggaaagttc cctgtgcccc gactgccaga gacgcattca
    43981 caccaacccg ctgcgcgtct ttgactgcaa ggtggaatcc tgcggcgagg tgattgacgg
    44041 cgctccccgc attttggact tcctgtgcgc ggactgttcg gagcattttt cccgggtcaa
    44101 aagctatctg tctgacctga atctgcccta tgaaatcaac ccgcgaatgg tgcgcggtct
    44161 ggattactat acccgaacgg cttttgaggt gaaatccggc gggcttggcg cgcagaattc
    44221 gctcgcgggc gggggccgct atgacggcct ggtccaattt ctggggggcc ccgacatcgc
    44281 cggcatcggg ttcggcatcg gaatggaaag gctgatcgcc tgccttccgc ccgacgaccg
    44341 gaatccttat gcggcggaaa tctttattgc cgccctgggg gagcgcgcgc agaaaatcgc
    44401 ttttactctg acgcaggaac tgcgccgaag gggaatttgc gcggccatgg attattccgg
    44461 caaaagcctg aaaagtcaga tgaagcgcgc ggacaagctc cacagcgcct acacgctgat
    44521 cttcggggac agggaaatcg aggaaaactg cgccgaactg cgcgacatga acacgaaaaa
    44581 tcagcaacgt ctgccgctgg accagcttct ggaagcggtt acggatattt taaaaagaga
    44641 ggtaaaggtt ttgtgagtcg ttttattgtg aaagaaaaaa gaacccatta ctgcggaagc
    44701 gtaaccgtcg ccgatgaagg caacgaaatt gttttaatgg gttgggtgca ccggcggcgc
    44761 gatcacggcg gcgtcatttt tgtcgatttg cgcgaccgcg aaggcatcgt tcaggtcgtt
    44821 tttcagcccg acgcgggaga agccgtccac ggagaagcgc acaagatacg cagcgagtac
    44881 gttctggccg tgaaaggcac agtgcgcaga aggccggagg gcatggataa cccggcgctg
    44941 cccaccgggc aggtggaagt cgtcatttcc gaactggaaa tcctgaatga atccaaaacg
    45001 ccgcccttct cgtttgacga tgaagacatc tcagaaaatg tccggctgaa ataccgctat
    45061 ctggatttgc gccgcccggc cattcaaaag aacctttttt tgcgaagcag actggccatt
    45121 gccacgcggc agtatttcaa tgaccacgga ttcatcgaag tggaaacgcc ctttctgacc
    45181 aagagcacgc cggaaggcgc gagggactac cttgtgccca gccgcatctc caaaggcatg
    45241 ttttacgcgc tcccccagtc tccgcagatt ttcaagcaac tgctgatggt ttccgggttc
    45301 gaccgttatt tccagattgt caaatgcttc cgcgatgagg atttgcgcgc cgaccgccag
    45361 ccggaattca cgcagatcga cgtggaaatg tccttcgtct cggaagaaga cattctccgg
    45421 atgatggagg gcctgatggc ggcgattttc aaggcctgcc ggaacatcga tctgcctctt
    45481 ccgtttcccc ggctgacgta cgccgaagcc gtcagccgct acggcaaaga caaccccgat
    45541 gtgcgcttcg gcatggagat cacggacctc acggccatcg tgaaggattc gggcttcaag
    45601 ctgttcgccg aagtcgccgc ttccggaggc gtcgtcaaag ccgtcaaagc cgaccaggct
    45661 tcttctcttt cccggaaaga tctcgacgct ctgtctgatt ttgtcgccat atatggcgcc
    45721 aaagggctgg cctgggccaa ggtcaacgcg gccgactgga cctcccccat cttcaagttt
    45781 ttgaagccgg aagaaacggc cgccatcggc cgggccatgg atgccaaaga aggcgacatc
    45841 ctcttctttg tcgcggacac gcccaaaaca gcctgcgacg cgctgggcaa tctgcgtctg
    45901 cacctgggca aaaagctgaa ccggatcgac ccggacgcgc tggccttcac ctggatcacc
    45961 gagtttccgc tgatggagta ttcggagtcg gaaaagaggt tcgtctccac gcatcatccc
    46021 ttcacctcgc cgtttctgga ggacatcccg ctcatgacca ccgatccggg caaagtgcgg
    46081 gccagggcct atgacctggt gctcaacggc tcggagatcg gcggcggcag catccggatc
    46141 caccgcaagg acatccaggc gcaggtcttc agcgcgctgg gcctgagcga tgaggaggca
    46201 aggctgaaat tcggttttct gctcgacgct ctcgaatacg gggcgccgcc gcacggagga
    46261 ctggcgttcg gtctggatcg gcttgtgatg atcatgacgg gagcggaatc gatccgcgac
    46321 gtcatcgctt tcccgaaaac acagaaggcc gcggacctga tgtccgacgc cccgtcgaag
    46381 gtgagcattg aacagctgat ggaattgtcg ctgaagatcg tttaattttc ccggcagtac
    46441 cggtcaattt ccagggcgac ctttttggca aaggccacgg cctcgacgac cgttttcggc
    46501 cccgtgacga tatcgcccgc ggcaaacacg cccggggatg aggtatggcc gttttcatcg
    46561 gcggcgacca gcccgcggtg tgttttggaa acgttgctgt cggccatcac cgcgccctgc
    46621 ggaccctgac cgatggcgac gataatcgaa tcggcgggaa tcttttgcgt gcgggaaaaa
    46681 tcctcttcgt aaaccgttcc gctttcggtt ttttcagcgc gaacggcaac acacttcacg
    46741 ccgtcttcct cgagcttcag cgcctgaaga agatggacaa attgaatccc ctcccggacc
    46801 gcatcgtcga tttcctgccg gttgccgaag atttcttcct cgagcagatt gttgatcacc
    46861 gtcacggcgc cgcccggttc gatgctgcga accgcgattc tcgcggcatc cagcgcgacg
    46921 tttcccgcgc ccacgacaac cacgttccgg ccgaggcggt aggcctccgg gcttttgagg
    46981 tagtcgatgg cgtaaaaaac attgcccagc gtctcgccca gcagaccgag cttgttgggt
    47041 ttggccgtgc cgaccgcgat gaagacggcc ttatagccgt cgatgaacat atcgtcgagc
    47101 gtgatggagg acccgataaa agtgttgggc ttgaattgca cgcccagttg gcgcaaaatg
    47161 ggatcgtagc ggtccacgat ttcccgggga agccggaagc cgggaatgcc gtagcgcaaa
    47221 acaccgccga ttctgtcgcg ggcctccatg agcgtcaccc ggtatcccat gagggacagg
    47281 agaatgctca tcgtgattcc ggccggaccg gatccgatca cggcaatgcg ggcgccgttc
    47341 ttttccataa cgggcggctc ataggttttc agaaaggtcc aggacaggaa ctgctcgatt
    47401 ttgaaaaagt ccaccggctc ggattttttg ccgagcacac agtggccaaa acagtttcgc
    47461 tcatgcgggc agacgatgga gcacacggcc gaaagcggat tgttggcaaa caggatttgg
    47521 gccgcctcct gctcttttcc ttcttccacg agcctcgtca cgtcgggaat cggcgtggat
    47581 acggggcagt gcttcctgca ctggggaacc ttgcatttca ggcagcgggc cgcaatctga
    47641 tcgatcaacg tgaaccatcc tcccttttgt ccaatcttca tagtccaaag cgcccggcgt
    47701 ttcaagaaag atttgaggaa aaaaacgttg ccgggagcct caaaggttgc tattgaatca
    47761 aaaacgcgga ggctttcgag atttgaagct tgaaaagaaa gacagccgcc gtgccgtgag
    47821 aaaaaaggga ggcagcatga aagaaatgga atatttaaac gcggcgggcg aagccatgcg
    47881 caaaatccgc aaaggagctt ttctgaccgt tcgcgcggcg gatgcgcaaa acaccatgac
    47941 catcggctgg gccatgctcg gcgtcatctg gcagaagccg gtcctgatgg tggcggtgcg
    48001 aaatacccgc cacactttcg gccttatgga aaaggcgccg gattttacgg tcaccgttcc
    48061 ttcgggcgac atgagccggg aaacggcttt ctgcgggacc aaatcgggcc gcgacctgga
    48121 caaattcgcg atgtgtcctc tcaaaacggc aaaggggcgg cacgtcgcgt ccccgattat
    48181 tcagacgccg ggaatgcatt tcgagtgcaa aaaaatgtac cagtccgcga tgaatccggc
    48241 ccatctggat tcaggcctgg accaggccat ctatccgaaa aaagactatc atacgctgta
    48301 cttcggcgag attctggcct gctacgagac cgaataagcg gcggcatttc cggccgcagg
    48361 gacgccctta atcgtcaaac agcgcccact gttcaaaggg gcgatgttcg tcctgcgggg
    48421 agagaggcga tatttcgctt agaaaacgcg acgggcagag ggtttgcacg cccgcggagc
    48481 ggctgtagtc caaaacggga tacgaaaggt aaagctggtc tttggcgcgc gtcaccgcca
    48541 cgtaaaagag tcttctttct tcttcttctc cgcccggctc ccgaagcgcc cgctccaggg
    48601 gaatcatgcc gtcggcgcac ccgatgatga aaacataaga ccattccagc cctttggcct
    48661 gatgaatcgt gcttaagatg accttgtcct cctgccgctc ttctatttct tcttctttgg
    48721 acatattggt taagagcgcc agttcgttta aaaaatcatc catcctctca aacctggccg
    48781 caaaattccc cagctggatc aagtcctctt cacgggcgta cgcatcggaa tagctgtcct
    48841 gcagataggt ccggtaatcg cctttgttta aaagcagatc aatgatcttt tccggtgttc
    48901 tgtcggggac gtccagcgca agcaatagca gcaaggtctt ccggcactga cctacgccgt
    48961 tttgcgccga tttgggcgcg gtttgtaaaa acgattccga aatcaaagtc tccaatggat
    49021 gatcctgctt tttaagccgg ctccagattt tctcaaaggt aatcttgccg atcttgggat
    49081 acatcatcag cagccttcgc caggccagtt catcatgggg attcaccaga acccgcatat
    49141 acgatgtcac gtctttgatg tgagcctgct caaaaaaacg gatgccggag cggatgtcga
    49201 atggaatgtc gcgccgcaca aattccatct gcgcttccat cgaatgatag tgcgcccggt
    49261 agagcacggc catctcgcaa aacggcaccc cgctgcggga gagctccgag attctttgcg
    49321 cgacgaagtc cgactgcttc aaaacatttt gagcggaaac gataaccggc ctcatgccct
    49381 tgttgcgaac ggctttcagc gccttgggaa actgattttc attgttgttg atgctcaggt
    49441 tggccagatg cagaatttcc ggcgtgctgc gatagttggt ctccagtttg aatattttgc
    49501 agtcgggata gcgctcggga aaatggatga tgttctcgaa attggcgccc cgaaaagaat
    49561 aaatgctttg tgaatcgtct cccaccacca tcagattgcg atgcccggac gccagcagat
    49621 cgacgatgtc ggcctgcaga atgttggtat cctgatattc atccaccagg acatgctcaa
    49681 accgccggga aagcgtctgt aaaatatcgg gctgctcccg gagcagcctg cggcagttgg
    49741 ccagcagatc atcaaaatcc attaccgata gatctttttt cttctgatcg tagaggcagg
    49801 cgatctgatg aatttccgcg cttaagtgca aaaagaaggg gtatctcgac gaaatcacat
    49861 cttccagcgc agtctctgtg ttcagcgcca gactgatcat ttccgcaata atattatttt
    49921 tggggaattt ggtcagtttg gtatcgatct ttaattcggc catcgccgat ccgatgacct
    49981 gtcgggcatc ttcactgtca acgatggaaa aactgctgtt gtagccgaga cgaaaagcgt
    50041 agcttctcaa aatacgatgg gcaatggaat gaaacgtgcc gcccatgatt ttgccggtgt
    50101 aatgctgaac caggctttcc acgcggttca gcatggatcg ggcggcctta tttgtaaacg
    50161 tcgccagaag aatattttcc ggccgtatgc cggattccag aagatgcgcc acccggtagg
    50221 tcagcgtccg cgtcttgccg cttccggctc ccgcaagaac aagaagcgga ccgccttctt
    50281 ccgtgacaac ccggtattgc tccggattca gttctttggc atactcaatc atcgactgtc
    50341 gagggctcag gcatgaaggg cctttttaat ttcagcaacc gtatctttag agctgagcga
    50401 caaaaaggtt ttcaacaacc cttctttttc ataaaaaccg gtgagcggcg ctgttttcaa
    50461 attgtaggtt tccagacggt tggtgatcgc ctcttcggtt tcgtcgtcgc gctgaatggt
    50521 ctgatggtca caaagcgtgc aggtgccgtc ttctctgggc ggattgcttt tgacgttgta
    50581 aatggcctgg caggcggggt tgcagcaggt cctgcgggtc gtcagccgat ccagaatcac
    50641 gtcgcgcggc acatccagat tggcgacaaa atccagcttg attttcagtt tggacagcag
    50701 tttctgaagg tcttcggcct gcggaatcgt acgcggaaaa ccgtccagaa taaatccctt
    50761 ttgacaatcc ggctcctgaa gcctcgcctc catgatgtcc atgatcagcg aatccggcac
    50821 caggtcgcca cgatccatgt attctttggc ttttttgccg agggcgctgc cctctttcac
    50881 ggcggagcgc agaatatcgc ccgtggagat ctgtatggat ccgtcatatt cggtcagcag
    50941 cttggcgatc gtgcctttgc cggcgcccgg cgcgcccagt aaaataattc tcattgcaat
    51001 cctccatcca tcaaatgttt aagctgcggc gcttatagtg caaaaccttc aaacggtcaa
    51061 ggcaattaag gttttaggga gtgatttgaa tttcaaaaag gtgcggccag tatttgccgg
    51121 tcacccaaat ccggttttgt tttgcgtcaa aagcgatgcc gttgagcaca tccacgcggg
    51181 cattggcggg cagataggag cgaagcgcgg aaagatcgat ccagcccgtt atcctgccgg
    51241 tttggggaga aatacgggca ataagatctt ccatgtaaat attcgcccag atttcccccc
    51301 ggacaaactc cagttcattg aggcgggaaa ccggccggtc gccgtcctgg acacgaatcc
    51361 ttctttggac ttgaaaggat ttcgggtcac ggatggtgat gattgaagag ccgtcgctca
    51421 tgagcaggct tttcccgtcg gaagtcaatc cccagccttc cccggaatat ttcattcgcc
    51481 tgatttcccg cagggaggcg gcatcgtaca ccagggcgtt ttcattttgc caggtgacct
    51541 gataaatcct gcctttcaaa agcgcgatgc cctctccgaa aaactccggt ggcagttgaa
    51601 tctgcctgag aactttcccg gtatcgatgt ctttgcaggc cagcgtcgat cggcccagca
    51661 aaccggtgga ctcgcacaga acaccgtcca tgacgatcag tccctgcgtg aaggcgtccg
    51721 aatcgtgcgg atggattttt tgaaccgcga cggcggagat gggcgccttc gttttcatca
    51781 gaagggaaaa cggattcttg ccggggggaa tcctctccgc gcccgtcagg cacgggagca
    51841 aaacacaaag cgaaaggagc agcaatgcgg caaatatttt ggggatattt cgggtcatct
    51901 tgaattaccg tccgggctga gggaaaagca tttccctcag ctcacgggga catcctcgtg
    51961 tctgtgcgac cggccgcgcc gtcatccgct gctgcctgca accggcaggg catccaccga
    52021 acgcaaataa aggggcgcgc accgggccgg atccagaaaa tccttgcgga aatatttttc
    52081 cagcgccagc cgcgcgacgg cggcggcgct gttaaactgc tgttcgaccg gcgccatttt
    52141 atttttcggc gaagacaggg gaggaatcag cccggcatag cggacggcgc cgtctccgac
    52201 atagatgacc ggctcggaaa aaggatcctc cacgctacgc gggtcctgag cctgcggcgc
    52261 cctgaccggt tggaggacgc cgtcctttcc gcgccggtag taggccagat acacctggcc
    52321 gcggcccgca tccatcatcg aaccgatcac ggcgtccggc tcctcagcgt tgagcgccag
    52381 agccgcaagc gaggaaactc ccgccgccgg ttttcccgtc gccatcatca gacctttgag
    52441 tgtgctgagg ccgatgcgca gtccggtaaa ggagccgggc cccagcgtgc aggcaaaaaa
    52501 tccaatgtcc cgcacggaga tccccgtctg ccggcaggcc tcgtcaatag ccggaagcag
    52561 cacttccgaa tggttttgct cccgatgaag aatggcgttg tataaaaccg cccggtcttc
    52621 gagcagggcg accgaaacgg ttttggaaga cgtgtcgaaa gccagggtca gcatattacc
    52681 tgaaaaattg taagaccttg ttgaccggtc cgatattgag cctttcgatg tcgatcatga
    52741 tgacgaaaag catcagcatc agcaaaaaga caaacccgat ctgcgaggcc cattccttga
    52801 ttttgaccgg aatttccctt cgggccgcca tttcgataag ataaaagaaa atgtgtcccc
    52861 cgtccagcac ggggatggga aacaaattga tgacccccag attgatcgaa agaatcgcca
    52921 taaaaaggac aaacggaata atgccttcac ggacctggtt gcccgccacc tgtgcgatga
    52981 agatgggtcc gcccagcgtt cggggcgaca gaaccccctc cagcattttc acaacggaaa
    53041 taacggtgag cttgctgatt tcccaggttt tctccaccgc tgaagccatg gccgccgtcg
    53101 gatttttctt ttcaatgacg gtctttccgg ccggtgaaat gccgatcagg taggtggatt
    53161 cctcttcgcc gaaaatgttt ttcgtcttgg tcagcttcgg tttcacggca aagattttct
    53221 cttccccgcc gcgccggatt cgaatggtga gatcggcgcc ccccgctttc aggatgacgg
    53281 gtttgatctc gtcccagaaa gtgatttcgc ggtcattgat ggacaaaatg gcatcgtctt
    53341 tgagaatgcc cgacgaggcg gcggcggaat cgggcgacag ctccccgatg accggaagaa
    53401 gattgggcag cccgtgcatg taaaccagaa aaaaaataaa gacggccaga agaaagttga
    53461 agacggggcc ggccagcacg atgagcattc gcttccaccg cggctgcttg taaaaagacc
    53521 ttttttcatc ttccgccggc agttcctcgg cgcccgattc gccgagcagt ttgacgaatc
    53581 cgcccagcgg aatccaggaa aggacatatt ccgtttcgcc gattttcttc ccgaaaattt
    53641 tgggtccgaa tcccagggaa aacttcagga cgccgacgcc gccggcccgc gcggccagaa
    53701 aatgccccag ttcatgcgcg aaaatcagaa tccccagcaa cacaataaac gaaataaaag
    53761 taaccaaacc atcatcctct cttgcgcttc ttcaaaatgc cggccgcgac ctttctggct
    53821 tcaccgtccg ccagcagaat ttcctccagc gaagggtccg gtatggccga atgcctctcc
    53881 agcgtttttt ctattacgtt gggaatctcg tgaaagcaaa ttttattttc aagaaaagcg
    53941 gccaccgcct cttcattcgc cgcattcaga accgccggcg ccgtgcctcc aatccgcgcg
    54001 gcctcaagag ccaggcgcag gcagggaaat tttttcgtgt ccggcgggaa aaacgaaagc
    54061 gggccggctt tggccagatc cagcgccgga aggtcgttcg tcatccggtc cggaaatgac
    54121 agcgcgtacg cgatgggaat cctcatgtcg gcaatgccca tctgcgccag aacggatccg
    54181 tccagaagct cgagcgccga atgaacaacg ctctgcgggt gaatcaaaac atcgattcgg
    54241 gaaacagcaa tgtcgaacag ccacttcgcc tcgattacct ccaggccttt gttcatcagc
    54301 gacgccgaat ccaccgtaat tttcgcgccc attttccagc gcggatgcct gagcgcctgc
    54361 gccggcgtca cttttttcag atccgcccgc gaaaaggaat aaaacggacc gcccgacgcg
    54421 gtgagaataa tccttttgat gtttgccggc ttctgtccgg ccaggcattg aaagatcgca
    54481 ctgtgttcgc tgtccacggg aagcacgcga atcttttttt gttcggcttt tttcgtcacc
    54541 agatgcccgg caatgaccat ggtttccttg ttggccagcg cgatgtcttt ccccgcttcg
    54601 atcgccgcga gcgtgggctt caggcccgcc gcgccggaaa tggcggaaag aacgatgtcg
    54661 gccgccgaat gcgccgcaat ttcccggact ccctcttccc cgcacaacac gcgaattttg
    54721 ccgcccggtc ccaaaagacc gcgaaggcgc tttgcatccc cgggcgtccg cacggaaacc
    54781 gccgcggggt gaaatgccag cgcctggcgg gccagaagct tcacattgcg gcccgcggcg
    54841 agcgccgcca cgcgaaattt attcggattt ttccggacga catccagcgc gcttgcgccg
    54901 atggatccgg tcgagcccag gatcgttatt tttttcatca gcccatcaca aagatgcggt
    54961 agtaatacac aaaaggcgcc aggaagatca ggctgtccat ccggtccatc agtccgccgt
    55021 gccccggcag caggtcgctg gcgtccttct tgccgtggct tcttttaatg gcggattcgc
    55081 aaagatcgcc cagttgtccg atgatgccgc ccacaaagcc caggaccgga aaatgaatcc
    55141 agtggacacc gggaagccag aaatagccaa acacgcaggc ggccagggtg gagcccgcga
    55201 tgagccccag cgtgccttca acggttttcc ccggactcac cgcctccagc agttttctct
    55261 ttcccaggga cttgcccacg tacagagcca ccgtgtcccc gataatgccg atgacgagca
    55321 ccagaagaac ccagtaaatg ccgccctcca gctttctcag caggacgaag tgcgaggtca
    55381 aaagcggaat gtacagaagg ccgaacatga ccttcatgac ggaggacgga tccgggaaag
    55441 actttcggac ctgccacaga aaaaccatga acacgatcag catcgcgaag gtcagaatcg
    55501 cggcgatcca ccgcgcatcc cctgcgagca cggcccaggg gatcaggaag gcgaagagca
    55561 ggctctcggt tttttccgcg cgatgcccgg ggccgaacac aatgccgttg tattcccaaa
    55621 ggccgatgac gatcaccagc gcaacgatgg cggtcaggac gaccggcgat cccatgacaa
    55681 tgatcaggag cagcacggcc gccagcgcca gaccggtcat ccatcgctgc agattggagt
    55741 tatgggtcgc catctctcac tcctttagac cgttatttcc tgccggaaac ctgatcactg
    55801 gttaagccga aacgtctttc ccggctctga tatgcggcaa tcgcctttaa taaatcgtct
    55861 tttttgaaat caggccacaa aacatccgta aagtaaagct ccgtataggc catctgccag
    55921 agaagaaaat tgctgatgcg atattcgccg ctcgtccgga tcagcaggtc ggggtcgggc
    55981 attccgctcg tgtacagata gcgttcaaac acatcctgat ccacggaatc ggggctcaat
    56041 tttccttccc ggctgtccgc cagcattttt ttgacggccg cgagaatctc gtcccggctg
    56101 ccgtagctca gcgccaaatt caaaaccatc ttgttatttt gccgtgtcag cttctgaacc
    56161 tccagaagtt tttcgcgcac gggcgcgtag agccgatcga tgtcgccgat agtggtcagg
    56221 cggatttcct gcttttgcat cgtcggcgct tccttgtcca ggtaatccgc aaggagattc
    56281 atcagcgcac tgacttcttc gcccgggcgg ccccagtttt ccgaggagaa agcgaacagg
    56341 gtaagacagg aaatccccag ttcacggcac gccgtaaccg ttgtccggac agcctgcgct
    56401 ccctttttat gcccccggat ccgccccatg gcgtgctgct tggcccagcg gccgttgccg
    56461 tccataataa tcgctatgtg gcggggcagt tgattttgat caatctcagg catttgaact
    56521 tgctttgtcc tttaaaaaca aatggggagc tttgactccc cattgttcag gtttatcgtc
    56581 tgaccaattt aaatctccat gatttctttt tctttggcgg ccagaatctt gtccgtcttt
    56641 tcgatataac ggtccgtcag tttctgaact tcatcctggg cgccgtgcat ttcgtcttcg
    56701 gacattttgt tgttcttttt caattctttg agcgcttcat tggcgtcgcg ccgctcgttg
    56761 cgcagcttga ttttcccctc ttccgccatt ttcttcacaa ccttgaccag gtcctttcgc
    56821 cgctcctcgg tgagctgcgg aatggaaagg cgaatcactt tgccgtccgt cgagggcatc
    56881 agccccaggt cggatttctg aatggccttt tcgatggcgc cgatcgtcgt aacatcccag
    56941 ggagcgatga cgatcaggcg gctttccgga acggaaagat tggccacctg ggcaatcgga
    57001 gtcggcgttc cgtaatagtc caccttgatg ccgtcaagca gggaaaccga cgcccggccg
    57061 gttcttactc tgctgaatga tttctccagg gaagagatgg ttttttccat attgtctttg
    57121 aaccttgaaa aaatctgctc tttcatgatc attctcctac gtacgttccg atttttttac
    57181 cgcagacaac gccgcgaatg ttgcccttct tctgcaggtt aaagacgatg aggggcaggc
    57241 cgttgtcgcg acaaagggaa atagccgtcg aatccattac tttaaggttt ttggtcaata
    57301 catcgatata acttattttt ttaaacatta ccgcagattg gtctttgacg ggatccgcgc
    57361 tgtagacccc gtccactttc gttgccttca ggatcacatc cgcccggatt tccatcgccc
    57421 gcagagatgc ggccgtgtcc gtggaaaaat agggatttcc cgttccgccg gcgaaaatga
    57481 caatcctgcc cttttccaga tgacggacgg ctttccgctg aataaaaggt tccgcgattt
    57541 catgcatttc aatggaggac tggacgcgcg tcgcaacacc ctttgcttcc agcgcactct
    57601 gaagggcaag gctgttgatg accgtcgcca gcatccccat gtaatcggcg gtcgtgcgct
    57661 ccatgccccg ggcgccggcc tgcacgcccc ggaaaatatt tccgcccccg atgacaatgc
    57721 cgatctgaat cttcaggtcc gcgagagacc ggatttcatc cgcaatgtaa tttgccacat
    57781 ccggatcaac gcccgtggac tgtttgccca gaagagcttc cccgctgagc tttaaaagga
    57841 ttctttggta tgcggccttt ttcttttcgc tcattattgc ttgatttcct cgccgagctg
    57901 gaagcgggca aaacggcgaa gaacaatatt ttcgcctgtt ttggcaatca tgttgctgat
    57961 cagcgttcca acggtgatgt cctggttctt gacgaatttc tgttcgacca ggcaaacgtc
    58021 ttcgtaatat ttcttcagtt ttccttcgat gattttgtcc cagattttct ccggcttctt
    58081 ttcttcgcgc aactggctgc ggtaaatttc cttttcccgg ttgagcgcgt cttcggcgat
    58141 ttcatccgga cgcacgtaca ggggattgga tgcggcaatg tgcatggcga tgtcccgcgc
    58201 catggtctga aagtccgccg tcttcgccac aaaatccgtc tcgcagttga tttccaccat
    58261 cacgccgatc cggccgccca tgtggatata ggaagcaatg gttccgtcct tggccgcctt
    58321 ggacgagcgt ttggttgccg ccgaaagccc cttctttctc aaaaagtcaa tcgctttttc
    58381 aaaatcgccg tccacggccg caagcgcttc cttgcagtcc atcatgccgg cgccggtttt
    58441 tgttctcaat tcttttacca tggttgacgt gatttccact atcttttcct cctggaattc
    58501 tttggtctgt gtttgctttt cacgcatgcg agcgtctgat cgccgaaaac ggcggcacct
    58561 tccatcgcct tgcggggtgt gcttttcgcg tgtatgcggt gtatgcttct atgaagcggg
    58621 cgcgggagag ccgttttcca tctcgacgct ttccggaatg tccgcttccg gatctttgtt
    58681 ttccgcggcg ggcgctccct cttccttgtt ggattccgcg gacagctttt cttcaaagcg
    58741 tttcctgcct tcgatcaccg cgtcggcgaa cttcgacgag aacagcttga tggcccgaat
    58801 cgcgtcatcg tttcccggaa tgatataatc gatgtccgtc ggatcgcagt tggtatcgac
    58861 gatggcgacc aggggaatct tcagtttttt cgcttcctgg accgtgatgc gctcccggtt
    58921 gggatcgaca atgaaaacgg cggcgggaag ggacctcatg tttttaatgc cgcccagaac
    58981 attgtccatc ttgtccatct ctttttgcag cttggtgatt tccttcttgg gaaaagcctt
    59041 gacggagtcg tcggcgaaca tcttgctgag gctgttgagg cgatcgatgc ttttcttgat
    59101 ggtgatgaaa ttggtcagca tgccgcccag ccagcgctga ttgacgtaag gcatgctgca
    59161 gcgcgtcgcc tcttcgcgaa tggcctcctg ggactgcttc ttggtcccga caaagaggat
    59221 ttccctgccc tggctgaccg tgtccacgac aaaattatag gccgactgga acatgccgac
    59281 ggtctgctgc aggtcaatga tgtagatgtt gttgcgggcc ccgaagatat agggcttcat
    59341 cttggggttc catttgttgg tctggtgtcc gaaatggacg ccggcttcga gcagaagctt
    59401 catggaaatg gctgacataa attttttctc ctttgttttt ttccgccacc cccgtcgtct
    59461 tgccggcgga cttgagcgtc aagcaacccg ccgtcaatca ggggatgtgt gaaatttttt
    59521 gggcaattat cataaatcca tggatcaagc aagcgcaaat aaccttttcg gcaataaaat
    59581 taacgttctt ttaccgccct caagtggtgt aatcggcatt gattttcacg tagtcgtggg
    59641 aaaggtccga ggtgtacacc gtgtaggact tgtcgccgcc ccccaggctg acgcggactt
    59701 cgatgctgcc gccctgaaaa atttccttca gtctggccgg cgctatattc gcgggcgcgt
    59761 cctgggcaaa gacggtcata tttcctatcg ataagctgac tttttctttt tccagcagga
    59821 tgcccgaagc gccgacggcg gacagaattc tgccccagtt cgggtcttcg ccgaaaaacg
    59881 cggttttgac caggttggaa ttggcaatcg aatacgccac gcgccgcgcg tcggattttg
    59941 acttggcgcc ttccaccacg acggtgatga atttggtcgc gccctcgccg tctttgacaa
    60001 cggccgcgga cagatccgcc atgacgtccg tcagcatctc ccgaaaccgc tgaagcctgg
    60061 cctgagccct ctccagcgga ttgttgccgg ccaggccgtt tgccagaata atcgcggtat
    60121 cgttggtgct catgcagccg tcgacgctga cggcattgaa cgtggaatcg atcacctgat
    60181 gaaagacggt gttgagcgcc ttggcgtcga tgagcgcgtc ggtcatgacg taggtcaaaa
    60241 gcgtcgccat gttcggctcg atcatgccgg cgcccttggc gatcccgcag atcgtaatgt
    60301 ccttggcgcc gacaatgccc ttgcgaatgg agatcttggg aaatttgtcc gtcgtcatca
    60361 tggcggcctc cgcgtcctcg atgccggatt cgttgagccc cttgaccagc ttgccgatgc
    60421 cggcttcgat ttttcggacg ggaagcctcc tgccgatcac ccccgtggaa caaatcagca
    60481 gatgctcttc ggggattttc aactgctttg ccgccgccgc cgaaacggcc agagcgtccg
    60541 ccatgccttc tttgcccgtc gccgcgttgg cgcagccgct gttggtcaga atggcctgcg
    60601 ccgttccccg tttgactctt tcggcatcga tcagcacggg cgccgcctta aaggtgtttt
    60661 tggtaaacag cgcggcgact cttgccggaa cggtggaaca gatcaacccc aggtccttcc
    60721 ggtcgccgcc cttgatgccc gcggatacgc cattggccag aaaaccggga acgctgaatt
    60781 tatttgctga tttcgtcatg accgccactc ctttgcattc tcaagcgccg cagcatttct
    60841 tgtatttttt gccgctgccg cagggacagg gatcattccg gccgatcttt tcggttgccc
    60901 gtttcaccgt ttgattgacc ggcgtgtctt cacctcggct taaaatataa tcctgctttt
    60961 gtttctgcct gatttcttcg acttcttctt ctctttgaat ccggaccagg cacagttttt
    61021 ccagcgtgtc catcttgatg cggctgatca tttccatgaa catcgcatac ccttcgcgct
    61081 ggtattccct taccgggtcc ttctgtccgt atccgcgcag gccgatgcct tccctcaggt
    61141 ggtccatgga cagcaggtgt tctttccagt gggtgtcgat cgcctggagc atgatgactt
    61201 tcatcagata cgtcatcagt tccgggccga attctttttc cttggcgcgc agatactcct
    61261 gaactttttc cagaatcagg cttcggacgg catcggcgga cgtcaggtcc tgtgcgtttg
    61321 cgatattgag gcgcagtttg aactgctgaa ataccgcatc ctcaagtgat ttgaggttcc
    61381 aatcctgagg gtgattcttt tcgccgataa attcagcaag cagatcgccg gcaatttcct
    61441 caaccatcgc ttcaatatca gaccataagt cttctccacg caagactttt ttgcgctgtt
    61501 cgtagattac ctttctctgg ttgttcatca cgtcgtcgta atcgagcaga tgcttccgaa
    61561 tgtcaaagtt ctgcccttcc acccgtttct gggcgttttc gatggcccgg gaaatgtatt
    61621 tgtgctcgat cggctggcct tcttcgatgc ccacggtgct catcaccgag gagatccggt
    61681 cggcgccgaa aacccgcagc aaatcatcct ccagggaaag atagaagcgc gacgacccgt
    61741 tgtctccctg gcgtccggac cggccgcgaa gctgattgtc gatgcgccgg ctttcgtgcc
    61801 gttccgttcc cagaatatgc agaccgccaa gctccgcgac tttttcaccg agcttgatgt
    61861 ccgtgccgcg gccggccatg ttggttgaga tcgttacctg tcccgcctgt ccggcctggg
    61921 cgacgatttc cgcttccttc tcgtgatttt tggcgttcag cacatggtgc tttaccccgg
    61981 cccgggtgag atacttggag agcaattcgg atttttcaat ggaaatcgtg ccgatcagaa
    62041 ccgggcgtcc ggctttgttg agcgccttga cttcctcgat cacggccttg atcttttccg
    62101 gctcggtttt gtagatcagg tcgttgtgat cctggcggat catcggcata ttggtcggca
    62161 tcaccaccac ttcgaggttg taaattttct tgaattcctc cgcttccgtg tcggcggtgc
    62221 cggtcattcc ggccagtttc ttgtacattc ggaaataatt ctggaatgtg atggaggcca
    62281 gcgtctgatt ttccttttcg atcttgacct tttccttggc ttccagcgcc tggtgcagac
    62341 cgtcgctgta gcgcctgccc ggcatgaccc ggccggtgaa ctcatcgacg atgatcacct
    62401 gaccctcttt caccagatag tccacgtcgc gtttgaagag cgtgtgggcc cgaagcgcct
    62461 gattgacatg gtgaacgatt tcaatattgc gcggctcata gaggttctgg acgttcagaa
    62521 gctgttcaac atgagacacg ccttcttccg tcaggacaac cgttctgctt ttttcttcga
    62581 ttgtgtagtc cgcgtctttg cgcagccggg gaatgatctg gttgattttg tagtacttgt
    62641 ccgtcgactc ttccgacgca ccggaaatga tgagcggcgt gcgcgcctcg tcgatcagaa
    62701 tgctgtccac ctcgtcaacg atcgcgaaat tgaattcccg ctgaacataa tcctccaggc
    62761 tgaatttcat attgtcgcga agatagtcaa acccgaattc attgttggtg ccgtatgtta
    62821 tgtccgccgc gtaagccttg cggcgctcgt cgtcgtccat gccgtgcacg atgacgccga
    62881 ccgtcaatcc cagaaaatga taaatcgggc tcatccattc cgcgtcgcgc cgggccaggt
    62941 agtcgttgac ggtgaccaca tggcagcctt tgccggaaag cgcgtttaga tacaaaggca
    63001 tcgtcgcggc cagcgttttg ccttccccgg ttttcatttc cgcgattttg ccttcatgca
    63061 gaacaatccc gccgatgacc tggacgtcga aagggcgcat catcagcgtt ctgcgggaag
    63121 cctctctggc cacggcaaac gcctccggca gaatatcgtc cagcgttaat ccgtttttca
    63181 gtttttccct gaaggcggaa gtctttgcct tgagctcggc gtcgcccagc gacatcagct
    63241 ccttttcgtg atgattgacc tcatggagca gcaccgataa tctttttaat tcacgatcgt
    63301 ttttggttcc cactattttt tttagaatcg cgttcagcat gacatccctt taaataatcc
    63361 ggcttttacc gggtgatttt agcatattta gccgcctaac aaaaacattc atttgcctga
    63421 aacatattct tttcgcatac ggctgcgctt acgcatgccg cccggcgcct gcggcctgat
    63481 gtctggtcaa tttgtcgggc tggattattt caagtatttt ctcggattga ctggaacatt
    63541 gttcacaaaa acactgtaat gaagatgagc cccggtgctg cggccggtat cgccgacata
    63601 tccgatcacg tccccttttc tgacctgtac gccgtctttc acggcggcct ttgacaaatg
    63661 cgcgtagctc gtcgaaatcc cgtacccatg ctcaattttt acgacattgc cgtcatcggt
    63721 gcgataggcc gcctccagaa caaaaccgtc cgcgggggcg ataatttcct tgcccgcccg
    63781 cgacgcgatg tcgatgcctt tgtgaaactc gccgccgctt ccgaacggat tgcgccgcat
    63841 gccgaattcc gaggtcaccc acccatggac cggccacagg gaaggcgtcg cagccaaaag
    63901 cgatttctgc tcccggagaa atcttaaaag ctcgttgaag ctcatttctc tctcgtcggc
    63961 ttccgcgttc agccggtcga tgttttcgtg catgccggaa accagtttct cccggtcctc
    64021 ttcaatgagc tgacgcaggc gcatctgatc gttattggac cctccgatgc ccagcggtgt
    64081 ttttgcgtcc tgcccgatct gttcggttgc cagaatccgc aattttttgt caaattgctt
    64141 gatctcctcc atgcggtcgg caaactgatc gatttttatg gccagctcat tcatctcttt
    64201 tgtctgctgg gccgtctgct gccgaaggcg ggacagctcc atccggtccc gggtaattcc
    64261 ggcataatcg taaataatgt aaagcgttag aagaaccgcc aggatggaac cggcaataag
    64321 gccatagatc agtttgcccg aaagagaaat cttggtcaca gaactctttc ttcggggtat
    64381 aatcatcagt gtaaaaaaat tatcggacat tttccccctg aaaagctgaa gtgaagagga
    64441 tgaacaggta taaagttttg catcccctaa cacagggaaa aatcaaagtc aagaataagt
    64501 cttggcgcct ttgtgcgact aaacaattat tatcaagcta attcaatgac ttgtgttatc
    64561 caggccgtcc tgtttcatac cgcgcacaaa gaatgtcttc ccggaagcag ctccattttg
    64621 tattttcgtc tggggagcct gtccatggtt aaggcatttt tgaaatcgcc aagccccgtc
    64681 acttccatcc ccgccttcag cgaggcctgc aaaagccgcc tgaactgctc ctcaaagacc
    64741 cctccttcca cttcggcgtg gaccggaagg acatggtcgc ccccgtcgtt catcaagccc
    64801 aggatcacgg ccgggccgtt ttgaagatca agctcttcaa aacagggcag atcggagggg
    64861 atttccggaa gattgagcgg atcgtgaaca aacggcgctt tggcccgcgt acagctcaga
    64921 tatgcgaacg gatatttccg aatgatccgc aaaacgcggt cgtcaatcag ccaggaaggg
    64981 gcgccgaacg cggccggctt ttgtcccgtg agcttctcaa agcctttgag gcccctgtcg
    65041 aaccactctt cgatccacgc ctcggatttt tccggcaggt catcctgcca gcggcggtgg
    65101 tcccaggcgt gaaactgaac ttcgtggccc tcggcaagga tttgacggac cagcccggga
    65161 aaggaaagcg cgatcatcgg cgcgggaaga agcgtcccgt aaagggcggt tttccagccg
    65221 tacagactgg gcgcccgggt gcgcagcatt tttttcagaa acagggggtt tttaatcatc
    65281 tgcagcgcgg cgcgtccgga attgtccggc ccgatgctca gaaagaaagt cccccggacg
    65341 gaaaaatcat tcaggacgcg caacaggcgg ggaacgccgt ttttcattcc cgcatacgtg
    65401 tccacatcga tttttaatcc aagcctgccc atcgatcctt ccgaattcaa tccggttaaa
    65461 gcgttgccgc ctggttttcc tccagaaacg aattgagcgt caggcgcagg gattcctgca
    65521 aaccgatttt gggctcccag ccgagcagtt tcctggcctt ttcgatgcgg ggttttctgg
    65581 tgtaaatatc ctggtagcct tttccgtaat agacgtccgc gggaatttca ataatatccg
    65641 aataaaccga atcctgttga tggtcggggt gctcctgaaa cagctttttg agcagcgccg
    65701 ccagctcctt caccgagcat tcattgtccg gattgccgat gttgatgatc tgattcttgc
    65761 agacatctcc tttgttttcc agaatcttca tgagcgccgc gatgccgtcg tccacgtaag
    65821 tgaagcagcg cttctgccgg ccgccgtcca ccagattgat cggccgcttc aacagcagct
    65881 cggcgatgaa ctgcgtgacg acgcgcgagc ttccttcctt ggccgtatcc agcgaatcca
    65941 gcctcggccc gatccagttg aagggacgga aaagcgtgaa ttccaggctt cccctcgttc
    66001 cataggcata aatcacacga tccagaagct gtttgcagca ggaataaatc cagcgctcct
    66061 tgcgaatcgg cccgaccgac agatagcttt cgtcttcgtc aaactccgga tcggagcagg
    66121 ccccgtaaac ctccgacgta gaggggaaaa caacgcgctt gcggtatttg acgcactgct
    66181 tgacaatatt gacgttcatt tcaaaatcca ggttatagac gccgatgggg tcttccacgt
    66241 aggccttcgg agtggcaatc gccaccagcg gcatgatgac gtcgcatttt ttgacatggt
    66301 attcaatcca ttcccggttg atggaaatgt ccccttccaa aaaattgaag cggggattgt
    66361 gcagggcggc gccgatccgc tccgttccca gatccattcc aaacacctgc cagtccggct
    66421 tctcagccag aatccggttg acaagatgat gcccgatgaa cccgttgacg cccagtatta
    66481 aaatcttcat gtaagttttt tcacctttcc ctgtttaaaa tagttcccga tgcccgcatc
    66541 cttccatctt gccccggatg cttcgatctc ctgaagcctt atagcaccat cgccggtttt
    66601 cacaagcacg gattgcccgt cgaggatcac gtcgcccggt ccgcccccgg aaacagatga
    66661 aaccggcctg gcccgccaga tcagaatttt cccgccgttt tcggcaaagg caaacgctcc
    66721 cggatagggg tccgtcacgg cgcggatcaa attgtagatt tcgacggcgg ttcggtccca
    66781 ggagatgcgc ccgtcctccg gtttccgtcc gccgtaatag cttccccgtg acaggtcctg
    66841 cttgatgcgc ggaatttttc ctttcttcat ttcggggagc agctcgtcga gcagcccgcc
    66901 ggccgcctcg cagagcttgt cgtacagcgt tcgcgccgta tcctcaaaat cgatgctcac
    66961 ggccctccgg ccgacgatgt cgcccgcatc cggtttttcc accatgcaat gcagcgtgac
    67021 gcccgtctct ttctctccgt tgaccagaac ccagttgacc ggacacctgc cccggtaaga
    67081 cggcagaagc gagccgtgca gattgaaagc gccgatgcgg ggaatcgcca gaacctccgg
    67141 acgaaccatg ttccggtagt aaaaggaaaa aaggacatcc ggctgcatgc gtttgatttt
    67201 ttcaatccac tgaggcgagt tgaccggatc ggtcgtgcag ccgattccct gcttttccgc
    67261 ccagtccttc acggagccga accagcagtt ttccttcggg tcgtcctcgt gcgtgaagac
    67321 ggcggcaatg tcaaagccgt gccgcccgag ggccgccagc cccgccagcc ccatattgtg
    67381 ataggccaga acaatcgctt tcacggacgg tcaccctcat gaccgtaaac gcgttcgatc
    67441 acgaattccg gccttttgcg gacttcccga aagatccggc cgatatattc gccgatcacg
    67501 cccagagcaa aaaactgaag gccgacaaag acgaacaaaa tcgcaaacag cgtgaaaatc
    67561 ccttccgccg cccagcgcac gccgtacacc aggcgggcaa ttgccagaaa cgcgccgaag
    67621 cacacgccca gaagcgacat cagaatcccg ccgtacatga tcagcttgat cggaaaatcc
    67681 gaaaaggacg ccaccaggtc gaactgaaga ttgatcagtt tgcgaagcgg atagttggat
    67741 tttcccccga agcgctcttc gtgcgccact tcgatttccg tgacgcgctt ggcgaaaacg
    67801 gtggccagag cgggaataaa ggtcgcctgt tcgtggcagt cgagcatccg ctccaccacc
    67861 gggcggctgt aggcccgcag catgcagccc cagtcgcgca tgctcacgcc ggtgattttc
    67921 cgcgcgacca cattgatcat tttggagggc agaatgcgca gaaaagaatc cttgcggctc
    67981 ttgcggatcg tgccgaccac gtcgtattcc ccctcttcca tcgtacggac caggttgggg
    68041 atttcttcgg gagggttctg caggtccgcg tccatcgtca gcacgatgtc gccccgcatc
    68101 accgaaaacc ccgccatgat ggcggcgtgc tggccgtagt tgcgcgtcag ctccaccacc
    68161 cggaccgacg ggtgcgacgt gaactccttc aaaagggcca gcgaccggtc gcggctgccg
    68221 tcgtccacca ggataatttc aaacggtttg gccagagact ccatcaccgg gagcagacgg
    68281 cccatcagcg cctggatatt ggcctcctcg ttgtacaccg gaatcaccac cgagatccga
    68341 atctcagaca tttttcaaaa tctcctttat ggctccgcaa acgtagtgaa catcattttc
    68401 cgtcatatcc ggaaacagcg gcagggaaat cactctgccc gccgcccgac tggtttgggg
    68461 aagcgatctt gcctttacgc cgaatttttt ctttacataa gacaaagcgt gggcgggagg
    68521 aaaatgcaga ccgtatccga tattgtactg cgacagcttt tgcataaaga catcccgcgc
    68581 gatgcccccg accttgacga tgaacaaatg ccaggcatga acgtggtcat aggacggaac
    68641 ctccggcaga tcgagcccct tcaccccccg cagtccttca aggtaaagcc ccgccagcat
    68701 ttgcctgcgg gcgttcagct cccgccaccg ctccatctga gccagcccca gcgccgccag
    68761 gagatccggc atgttgtatt tgtagccagg caccgcgatg tcataggagg gattcccccc
    68821 tttgccgtac cttttccaag cgtctctttc tattccatga aatctcagca gacgcaattt
    68881 cttttccagg gcggcctgat tcagcgtaat catgccccct tctccggtcg taatattctt
    68941 gatcgggtga aaggaaaaga tcgcgggatg gccgaagccg cccgcgtgcg tccccttgta
    69001 ataagtgccg accgcgtggg ccgcgtcttc aatgaccttc agggaatatt tgcgggccag
    69061 ccggttgatc cggtccatat ccgccggagc gccggcaaaa tgaacgggaa tgatcgcctt
    69121 tgtccgcttc gttatttttt cttcgatttc ttcgcagcaa agattgagcg tcccgtaatc
    69181 gatatcgaca aagacgggtt ttgcctgatg cagggcgatc atgttgacgg tcgaggcaaa
    69241 cgtcatcgag ggtgtgatca cttcatcgcc gggcccgatg ttcagggcct tcatcatcag
    69301 atgcattccg gccgtggctg aactgagcga gacggcgcga tccgcgccgg tgagcgcgca
    69361 caactgttct tcaaacgcct tgcacaaagc gccggtggtg atccagccgg atttcagaca
    69421 ggccaccaca ccgttgattt ccgtctcccc gatggacggg cggctgaacg gcagaaaatc
    69481 tttccgaatc ttcatagggc atcaatcctt tgcctgattg tgaatgcgca gcagataaaa
    69541 cgcgccgttt tcccagagaa cctccattcg ggcaaactgc gcttgcagct ctttgagcct
    69601 ttccgcatgt tgtgtaatac agtaaatttc cttttcccgc ctgccttttt caaaaaattc
    69661 ctgtgtcgaa agaaaatatg ctttcctttc ggcctccggc attttcatta tcccgtcgcc
    69721 cagttcgccg aaatcctcga caatcggcgt ccgtattttg ttataaaaat caatgccata
    69781 gaaattgaca cggtactgat acaaaagccg gtccggcggg atgtgccgtg caatcgcctc
    69841 tttcgccgta agcgcgctgc ggtagggaga cagaaaatca ttgagcggga aaagaagtcc
    69901 ggcaagcaaa agacagcaaa gcagatacat tgtggaaaac cagcccgcgc cctttcggcg
    69961 gaaaatccaa tccggaagaa acgtcatcag ccccgccgcc aggaacaccg gcagaacgca
    70021 gatccaccag gcatttgaaa tcataatgac cagcccccgt gaagggtcat gatactgctt
    70081 cagaaccggc ggcagaataa cagcggcggc aatgaccagg aactggcccc acaaaaggag
    70141 ccggtaaaac acttccctct ttccggccgg ctcagacggc gcttcttcat ggccgcgaaa
    70201 gatgttgccc gcgaaaagcg ccaggggcag aaataccgga gcaatgtagg tggccagctt
    70261 ggaggaagac acggtgtaaa agagaaaaac aaacaaaaac cagacggcca gaaaccggtt
    70321 ttcctctttc ctgaagagcc gctctttcca ttttgcaggc tgccaggcgc gaatcagaaa
    70381 aacggaccag ggcagcgtgc cgccgatcag cacgggcagg aaataataaa acggctccgt
    70441 cttgccgtgc atctgagtcg tgaagcgcag gaagtgctcc cgcacaaaga aaaaccacag
    70501 aaaatccgga ttttcttttt gcgccagata aagccacggg cagaccacgg ccagaaaaat
    70561 caaaatcccc gcgggggaaa tcagccgcgg aagcgaacgc cagcgcccct cccggatgag
    70621 ccagatgacc agaatggcaa aaggaaagac gacgccgaca atccccttgg tcagaaaagc
    70681 cagggcgcag gcaaaataaa acccgcaggc cagccatctt ttcttttcgt ccgccaggga
    70741 caggtatccc agccagatag ccaggcaaag aaacagcgtg agcggcatgt ccagaatatt
    70801 gattcggccc agcatgaacg ggaagacgga aatggtaagc acggccgcgg catacaagcc
    70861 ggttttttca tcccggaagc gccgcccgat ccagaatacc agcaaaatgc acccccaggc
    70921 gcaaaggccg gaaaacaggc gagcggaaaa gtcgttttct ccaaagattt taaaggaggc
    70981 cgccgtcgcc caggaaacca ggggcggttt ttccaggtag attgtatttt tgatttgggg
    71041 ggttacatag ttgcccgttt cgttcatcgc ggcggcaatg agactgtagc gcacctcgtc
    71101 cggctccatc tggggcagga cccccagcag ggcgacgtaa agaagaaaag ggatgatgaa
    71161 tagaatgtat tttttcttca cttgcgcatc aactcctgac gccggttttt gtttttaaat
    71221 gttcttcggg ccttcggccg cgccgactga agcccgtgag ccatttttat tgccttgtgc
    71281 ctgaatccag tccggcgccc gcccggtcaa agaagtactt cacccccagc gtgcagacaa
    71341 cccccaccac cgcgccggca gcaacgtcac tgagatagtg ggacgtgagc atcacacggc
    71401 tcgcggcaat gaccgcggcc ggaagaaaag cccaggcgcc gaagcgggga aacaaaatgg
    71461 tcagggcggc cgcgagtgaa aaaaccgtca ccgtgtggcc cgaaggaaaa gaattcaggg
    71521 cgtagcccgt ctcaaaaaaa tcaaagccga ataatccgtg gtcaaaaaga ttgaccggcc
    71581 ggtttcttcc ggcaatccac ttgagcagga gattgatcaa accggaagcc gaaatgcaca
    71641 ccagcaggaa gaacattcga attgtccatt ttttgttttt cgcgatcagc caaaagatca
    71701 ggagcgccgg cacaataatc atgaagtacc acctggactc cccggccatg gtgatccatt
    71761 ccgtcgcatc cagcacgggc cggccgagtc cgcggcaaaa atggaccagc ggaatatccc
    71821 agaaggcgta agcgatcccg gccagagccg cgcccgaagc gatcagcgca agcgcgatcc
    71881 atcttttttt cacggcttca ctccctgata attgcggcac cagacaaact ccgcccggtt
    71941 cacttttgaa ccgttcagcg tcaaatccaa acgatccgcc cgatcgacct gatcgcactt
    72001 cagaacaccc gccacatcga tttgttcaaa ctccgtattc agaaacagca ggtcatatcc
    72061 cagcggggat tttttctgcc agacgtcgaa ttgatcccgc ctttcatcca tcacaaaaac
    72121 ctcatgtccg taaggcaggc tgtagtacat gacccggctg cccatcgtcc agttcgtcac
    72181 ggcaatcgcc ccgggacggg aggaagggtt ctgcttcaaa agctcgtcgg ctttcgcggc
    72241 tatctcgggg tagccgtaaa tgtccctgaa gggagacttg taatcgggaa agggaatgaa
    72301 ctttccgccc agctccgcat aggccagcat cagggcgaag aagctgattc ccacggagac
    72361 atacagaaaa tttttccgcc atttcccgcc ggatgccagc agacgccagg ttccgatcgg
    72421 aatgaacaga gcgtaaaaag cggacggcca gtgaggaagc gttcgttcat acgccgatgt
    72481 aatcagaaaa aaaaccagga tggtcccgcc gaacaaaacg gacaggcgca ggtagtcatt
    72541 gtcgtcccga atgactttga aaaaaccgta aacggcgacg ataaacagaa acggactgta
    72601 agcgccgaac tgcgccgcca gcgactccag aatattttta aaactggtcg cgagcgatcc
    72661 gaacacatgg gacccctgat atttgaagct gatgaagtca tgggtcgcgt tccagtaaaa
    72721 caccggcgaa accagcgcca gcgcgatggc cgccgccaga aagagatatg gtgaaaaaag
    72781 aacgtcgtat cgctttttga gaaggaagaa gaccagcagg ggcggcacca gcagaatggc
    72841 cgtgtacttg gccaggccca tcaagcccag aaggatgccc aggatgatga aatccgccgg
    72901 ctgtttgcgg cgcgcaaggc gctccgcggc aaaaatcagc aggaaaacca gcaggagcag
    72961 gatggaatcc ggcagaagca tcaggcccag agcattgagc atgaaagaac agttcaacgc
    73021 gaaaacggac cacgaagacc agcgaacgga actcgtgatc ttcatcagca gcgcatagca
    73081 gcaccaggag gtcgcggcaa aaagcaaaac ggcgggcagc cgggccaaaa attcgttcgt
    73141 gccgaaaata taataaaaag gcgcatggag ccatcccgcc agaggcggat ggtccagata
    73201 gctccagtcc aggtatttgg cgtacaaaac atagtgggct tcatccaccc ccaggccaaa
    73261 caaaggcgca gcgatcaggc ggatcagcga aaaaaacagg atcaggatga tgatggattt
    73321 ctcagccttg ggcattgatg cgctccagat ggggggcaat tgccgcaaaa acctgttcgg
    73381 gctgaatcac ctgcagacag acattgtccc ggcaggcggt cttgcggtgg ttggacgcgc
    73441 tgacgcaggg ggaacaggcc agccccgcgt acatcggcgt ggtaatgccg agcgatccgt
    73501 aaagcgccgg cgtttccggc ccgaaaaaca caaaggtcgg caaatcggtg acggcggaaa
    73561 aatgcccggg accggagtcg ttggtcacca tgaactcgga gacgctgtac aaagcgggca
    73621 attgcgcaaa ccggactttt ccggcaaaat taacgcaccg gtcgtcgccc acctgttttt
    73681 gcaactgatc ggcctcctgt cgttcactgg gatcgccggt aatcaaaatc agcgccttcg
    73741 gacatttctc cagaatcatt ttcatcagcc ggacgtaatg ggccggcggc cagcgcctct
    73801 gaatcagcag ttcgctcgca ttgggattga tcaggatgat tttgtccttt tgcgggtcaa
    73861 agcccgcgcg gcattcgcga atgatcgtca gcacgtcttc cttttcgcgg gcggaaaccg
    73921 ccgctttggc cagaacaaga tcttcggcga acaccttttt tttggagtag ggaatttcct
    73981 ctttttccgc gatcagcgca ttgacaagcg cgacaaagtt cgccgcgatg tgaatgtggg
    74041 gattgtaggc gacccgatgg gtgaggaaac tgccgcggta caaaccctcg ttgaaaaacg
    74101 cgtgaaaccc gactttagcg ggcgctccgg aaaaaccggt caaaagcgcg gtcacccgcg
    74161 agaaaagctc gaggtcgatg acggcgtcaa tctttgtcct gcgcgtccag aaaaagaaac
    74221 gaagcgcgtc ccacatcagc ggcacaagcc cgttttcccg aaaagtgtaa atattttcat
    74281 ccgggacggt tttgagcagg tccaggctgg gcttgttttt tttgaaaatg gcgaagtaaa
    74341 gatccgcgcc ggtttcccgc ctcagctttc tcatcgcggg gtccaccagg acggcgctgc
    74401 ccatttccga gagctccatg aacaaaaccc tgcggggcga cgccttgccg ggtttgacga
    74461 accagcgaac cgttttatga agcagggtgc cgataaagca aaggggaacg ccaatataat
    74521 aatcgatccg gcgcatggta tccactttca tgatcagctc cattcccttt cgcggcaggc
    74581 aagttcggta aaatttgcga ggaatatagg caaaatgcaa ccgactgtca ataaaacaat
    74641 gaacgcctgc aggcacaaaa gccattttgc cgcccttcag gttttccttg tgtaaaattg
    74701 ccgatgcgtg atagtttttt caaaattttt tgaaacggcg atatgaataa aattatcaac
    74761 cgctacatat tccgggagat tgcccttccc ttcgtcatca ttctctttgt tctgactttt
    74821 gttttgctga tgggcaaaat tctgcagatc atggacctga tgatcaacaa gggcatccgc
    74881 gttctggaca ttgcgcacct gatcgccctg atcatgccca atttcctgct gtttaccatt
    74941 cccatcgcgc ttctggtttc catcctcatc gccatgggcc ggctgtccgc ggacaacgaa
    75001 atcaccgtcc tgaaggcgtc aggcgtcagc ctgctccagc ttttttatcc ggcggcggtc
    75061 gcctccctgc tggcgtttgc ctgcgccatg gtcatcggat attttctggt gccccagagc
    75121 aatttcgcca ccaagcggct cctctttgaa ctggcctcgc aaaacgccgg catcggcatc
    75181 aaggaaaaag ttttcaacgc cgacttcaag ggactgctct tgtatgccga caccattccg
    75241 gccgacgggg catcgatgga aaacgtgatc atctcggaca gccgcgtcct gggagaacaa
    75301 aacacgatcc ttgccaaaaa ggcctttctg gtggccgatc cgaagctgat gatcgtgaag
    75361 ctgcgcctgg agaacggctc gattcacacc gtgggcaaag atttgaaaaa ttaccggaaa
    75421 gtggatttta aaagctacga catcaacctc gacctgtcct ccacgatgtc gtctttcacc
    75481 gacgaagcca aatccagcac ggaaatgacg ctgtcggagc tcctggagcg aatgaaaaaa
    75541 ccggatctgg cccaagcagc catgcgcgaa ctggccatcg aagtccataa aaagttttcc
    75601 attcccctgt cctgtatttt cttcgggctg ctggccatgc cgctgggcat cacgcaccac
    75661 cggtccgtga aatcccgggg attcgccgtc ggcctcatca tcgttgccgt ctattacctg
    75721 ctgcgaatcg gaggagaggc gctggtggaa aacggcagaa tggacccggc actcggcgtc
    75781 tgggcgccga atatgctttt tgcgcttttg ggggtttttc tgttttacct ggcccaccgg
    75841 gagatttccg tttcccaggc tttgatcgcg ctctttcaag gcggaaagcc gagaggatga
    75901 actcgtgaaa ctgattgatc aatatattct gaaagaattt ctgcggtttt tcctgatcac
    75961 gtttgtatcc ttcatcgcgc ttttcctgat catcgatttt ttcgagaaaa gccgcatgtt
    76021 catgagcaac cacgccacgg caacccagat ggcctcctat tttttgtatt cgattccctt
    76081 cgtcatttcc atgaccctgc ccgtcgcggt cctgctggcg acactgctga cctacagctt
    76141 tttgtccaag tacagcgaga tcacggccat gaaagcaaac ggcatcagcc tgtaccgcat
    76201 ttcctttccc gtgctggccg tcgcggcggc cgtatccgtc ttcctgttct tcttcacgga
    76261 gctggtcacg cccgccggcg tccagaaaat ggagcatatt atcaaagtgg aagtgcagaa
    76321 gcagcaggcc ctgggatttt tcaagcagga tgaaatctgg taccgcagcc aaaatgccat
    76381 ctacaatttt aaaatgtttg acgttgagaa aaacatcctg caaggcgtca ccatcaaccg
    76441 cctgaacccc gactttacaa tgaaaacccg catcgacgcc gaacgggccc aatggaaaaa
    76501 ccaatcatgg gtgttttaca atctgctcgt cacaaccttt cagccaaatg cccccccgtc
    76561 gctggaatgg tcggaagaaa aagtcatcgc gctgccggaa ggccccaacg acttcaagat
    76621 tattcagaaa gacgcggaaa aaatgggata tttcgaactg cggcgctacg tgaaaaagat
    76681 tcaggcggaa ggctatgacg tctcgcgata cgcggtcgat ctgcagggca agatcgcctt
    76741 tccggtggtg tgtttgattg tcgttctgat cggcatgtcg ttttccatcc gctcggagcg
    76801 aagcggcgga atcatgcaga gcctggcgct cggattattc ctgggctttt cctactggat
    76861 cgttcacgca ttcagcatgt cgctgggcag atccggaacg ctgcccgttt tcctggccgc
    76921 ctggacggcc aaccttctgt tcggaggcct tggggcgttt ctgttctacc gcgtcagaac
    76981 ctgatcgaag ccccttccct atcggcggaa cgcctgagaa ataaacggcg aggcccgcac
    77041 aatctacttt cccgtcatta ctacgaaaat agaatcatgg taaagctttc aagcgcacct
    77101 aaaggtacgc actacataaa aaatgtaggg cgggtcttta gacccgcttc cgtcctaaaa
    77161 aatcaatatc ggtaatagtc cggcttgaag gggccttcca ccggcacgcc cagataatcg
    77221 gcctgctttt tcgtcaattt tgagattttg gcgtccagcc ttgcaaggtg cagcagggcc
    77281 acttcctcgt cgagcttctt gggcagcgtg taaacgccga cattatattt cttcgtggcc
    77341 agttcaatct gcgccagcgt ctggttggtg aagctgttgc tcatcacaaa gctgggatgc
    77401 cccgtggcgc atcccagatt gaccaggcgc ccttcggcca aaacgatgat cgaacggccc
    77461 gatttcagcg tccacttgtc cacctgcggt ttgatggttt ccttgcggca gaccctgctc
    77521 gtttccagat agtgcatgtc gatttcactg tcaaaatggc cgatgttgca gatgatggct
    77581 tcatccttca tcttttccat atggcggccc gtaatgacgt cgcagcagcc ggtcgccgtc
    77641 acgaagatgt ccgcggcgga cgccacctct tccagcgtcc ggacctcgta gccttccatc
    77701 gccgcctgca gggcgcagat gggatcgatt tcggtcacca ccacacgcgc accgaatccc
    77761 cgcatcgact gggcgcatcc ttttccgaca tcgccgtaac cgcagatcac caccatcttg
    77821 ccggcaatca tgatgtccgt cgcccgcttg atgccgtccg ccagagactc ccggcagccg
    77881 tagagattgt cgaacttgga cttggtcacg gaatcgttca cgttgatggc cggaaacagc
    77941 agttcgcctg ccgcctgcat ctggtagagg cgatgaacgc ccgtggtcgt ttcttcggac
    78001 acgccgcgaa tgccggcggc gaccttctgc cagcgcttcg gattcttctt ccaggcctgc
    78061 gcaagcctct ccagaatgat ggcgaattcc ttgttgtccg ccttttgccg gagcagtttg
    78121 ggatcctttt caattttgac gccctgatga ataaacagag tggcgtcgcc tccgtcatcc
    78181 acaatcatat ccggaccgga gccgtccggc caggtcagcg cctgctccgt gcaccaccag
    78241 tattcttcca gcgtctcgcc cttccaggcg aaaaccgccg ccgatccggc tttggcaatc
    78301 gccgccgccg cgtggtcctg cgtggagaag atattgcagg acgcccatcg cagatcggcg
    78361 cccaactctt tgagtgtttc aatcagcatg gccgtctgga tggtcatgtg caggctgccc
    78421 atgattttca gcccccggaa cggcttttgg cggccgtatt tcttgcgcac cgccatgagc
    78481 cccggcattt cgttttccgc caggtccatt tcttttctgc cccaggccgc ctgcgacaaa
    78541 tccgcaacct tatatttcaa acttttatca atttcctgaa aagccattat gttcctccaa
    78601 taattgtgaa aatttcttga tcaattgcgg actctgttaa catactcatc cggccagttc
    78661 aatatcttta ttctccccac gatcgggaat tttagtcgct ctcagcctca agctttttca
    78721 aaacaggatc atccgccggg aatatattgg tggtctcggt ttcgtcatca aaaataagaa
    78781 cggctgatcc atttttgagc ttgtttttta cctgttggaa acttgtttca agggaagcct
    78841 cgatggcgcc ataatccgtc ccgtttctgg aaacaaattc ttcgataacc ccttttaagg
    78901 ctttagcact gagtttattg acgggaataa tatgaatcgc cattgaaacc tgctttttaa
    78961 tatccgggat acttcttctg ccagtgatct ccatcatcat aaagtacaaa cagatcaccg
    79021 gcgatttctt caataactgc atttagttcc aattcttcaa gccagggcgc gggaattgcc
    79081 gccctcccct gcatggcccc cagtatattg ccggtgatcg aaccggtgct gtccgaatcg
    79141 ccggtatgat tgaccgccag gcgaacccct ttggcaaaat caccttccgc ggccagggca
    79201 cagtaaagag aaatcgccag cgcttcttcg ccaacccagc cgccacccat tttttcgatg
    79261 gtttccggag cgggctttgc cgttttcttt ttccaaagtg ccattgccag cgtgacggca
    79321 tccagtgttt cttcatgccc cttggctttc tttaagaccg tcaccgattc tttgatggat
    79381 tcctcaatcg taagtcctga aataatgccg cagatgatcg tcgccagaca tcccgcaggc
    79441 agataacccg acgggtggcc atgcgtaatc gcggcaatcc ggcagcccag atcgaatatt
    79501 tctttccata cttcgccgtg attcccctcg gattgcatag ctttagccgc aaacagaccg
    79561 accggcgcgg cgcgcatcac accgccgcat cccttgctgt tattgatcgg tttttccggt
    79621 gtccccatct tcgcgctttg cagagcggaa aggcaggtgt tgcccggcgc ccggcgatgg
    79681 tgcagatcgg caaccgtcag cagccatcca ttctgagcga gatcgaaatc ctcgtgggct
    79741 gatcttacgc cctgtgttga aagccagcgc agataagagc gatgaaccat ctcaggatat
    79801 tccgccggaa cgattccctg ctttttacga caccaggccc gcaaaagccc ttccgccgtg
    79861 aaaagcgtca tctgcgtgtc atcggtgatc gcgcctttcc tgccatagca gggcgcgtaa
    79921 ttctcaatgc cgttttcgcc gtattgcctg cggatggcgc cgatggaatc aaactcgacc
    79981 gccgcaccca gcgcatcgcc gactgcgccg cccagaagac agcccgaaaa atgctcaacg
    80041 ctcaaccgct cgttcaccgg cctgcaggag taaacatatt tttcctgatc ccgtgtttcg
    80101 attgtgtcag gacgggcatc gcgtaccgcc tgaattgcct tttcatgcgg catgcccagt
    80161 tccaccagca attgagccgc caccgtaccg gttcgtccca gaccgccccg gcagtggatc
    80221 a
//
LOCUS       DAOPAF010000003        57311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_634_length_57311_cov_50.418943, whole genome shotgun sequence.
ACCESSION   DAOPAF010000003 DAOPAF010000000
VERSION     DAOPAF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 57311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57311
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_57311_cov_50.418943"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLQ77_00615"
     CDS             complement(<1..1091)
                     /locus_tag="PLQ77_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPL09213.1"
                     /translation="MGDDIRQATTEGKIRDFETRAQSMMKMGGEKQVQKQHDGGKLTA
                     RERLNRLFDEGSFQEVQLFVKHHATLFGMDKKEIPADGVITGFGKVNGRIVFAAAQDF
                     TSAGGTLGEMHAKKIWKVMDMALDAQKPFVSINDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALTDWIFMVKNTSYMYITGPDVIKAVIGEEVSQE
                     DLGGAVAHASKSGVCHVVTENDEDCIQKIKGLLSYLPDSCHSPLPVAETTDNPDRECP
                     ELNKIIPDRAARAYNMKSIIKSVADNGEWFELHAQWAPNIIVALIRIMGRPVGVIANN
                     PMSYAGVLNVNASDKASRFIRFCDAFNIPL"
     gene            1285..2007
                     /gene="truA"
                     /locus_tag="PLQ77_00620"
     CDS             1285..2007
                     /gene="truA"
                     /locus_tag="PLQ77_00620"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPL09214.1"
                     /translation="MILEYDGAAYCGWQRQQNGLSIQQVVEEALARIIGENATVIASG
                     RTDAGVHAMNQAASFRSRTVLPANKIFLGVNSVLPDNIVVKDLQEAPPDFNALKDARG
                     KVYVYRIQNQRLRPALGRQYFWHVRCPLNLESMRLAAMKLIGEHDFSCFCATGCDIKN
                     RVRLLTVCSIVHQESGRLEITLESSGFLRHMVRNIVGTLVDVGRGKIAPESMPQILAS
                     RDRKQAGTAAPACGLFLKEVKY"
     gene            2012..2887
                     /gene="rfbD"
                     /locus_tag="PLQ77_00625"
     CDS             2012..2887
                     /gene="rfbD"
                     /locus_tag="PLQ77_00625"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPL09215.1"
                     /translation="MKVLLLGHKGMLGNDLMAQFRHRHDVIGMDREEIDITRASECRR
                     AVAETEPQLVINAAGYTNVDACETAKEECFAVNAEAVKNIAQACRDGNVPMIHYSTDY
                     VFDGNANHPYAEEDPCNPINTYGASKLAGEHHLKRLTDNFILIRTAWLYGAKGKNFVS
                     AILDRARETGKLTVVDDQMGSPTCTRDLAAATELLVDKNARGIFHVTNRGCCTWYDFA
                     RKILKEARMEQVELSPMKTSELQRAARRPAYSVLGMHKFVAATGKTMQPWQLAFSDYY
                     SFIASRTCLSPPGTA"
     gene            3003..5750
                     /locus_tag="PLQ77_00630"
     CDS             3003..5750
                     /locus_tag="PLQ77_00630"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HPL09216.1"
                     /translation="MRKIFKVVFWGLIPAAALMLQGCLVSTPSTLITPEIRSLYEGEY
                     TVDPYMKKHVPLSVAVLPFVDLSGSKEGANAMRKGFYNHFSSLPFKDMELYKVDDLLL
                     KAGLTDPDVIRKIPPRKLGEILGVDAVVFGEVSDFDKYFAVLYSQVAVGAEVKMYDAK
                     TGNFLWSGKHKVRKHEGGVSTNPVGIIATVIATALNVRDIQLLRANDDLFRDMVKTIP
                     TPSLAQAKRPPVITLLTQDTKGLPKKAGDEIKVVIQGTPNMQASFDIGQYRKRIEMQE
                     VEPGGYYGVYKVVPGDNIEKAIVTGFLTDDSGNTAHWVDAIGSVTLDTTAPDKPAGIT
                     STGRNQRVALRWEKNTAADLAGYLIYRSETPLSGYLQIGKTELNEYKDTDQNLANART
                     YYYRISAVDFAGNESRTEQAEGMPIAPGPTPVSGAIEADATWYSGASPYILESNVTVK
                     DKALLTIEPGTEIQSKGGGLVIEGRISARGDNDRIIRFDSAQDGQFWPGITFASVKDR
                     ENHLAFVRVQNARTAVFCQASSPTIEQSEFTKNHEAIKIQGVFSKPLVSRNTLYKNKG
                     AAISVSDGAAPRIVGNTINDNDHTGVVLHSAAAEITQNTITRNKNNGIAVKGAEAVIF
                     QNNLVDNQPYNVFGEMKGEPAKALNNWWGSAAMAAVLSGIHGRVDVSSVLDAPYPQGK
                     ALAVPILDQKLEGAIQADAYLILSRSPYRLVKDVVIDGGATLYIEPGVVIEYEQETSF
                     ITRNGGIVAKGTGEKPITFTAASASPAPGFYVSAVRFSEATKINSAFAYCIVKYATTA
                     FDIYTGSPEISHSYIAHASQNGVYCRKDAAPVLSHNTFAHNQGEGAVKCVGSANPKIF
                     QNNFIDNAVAIQSFSSIFIDARNNWWGASPPDPKIFWGENINTNPWLEQANPEAFTER
                     R"
     gene            5796..7763
                     /locus_tag="PLQ77_00635"
     CDS             5796..7763
                     /locus_tag="PLQ77_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPL09217.1"
                     /translation="MLWVIYFRNASLSFLDKTLFDRIQWTIHPKSRIGLVGDNGAGKT
                     TLFRAILGEVPLDSGLIEIPGRKQKKIGYLPQDLVELESTGLIEYLRRKSGIAGLEED
                     LRSLERQMAQAPSHSREYGELSSRYAQATAEFSAREGYAFEARAKQILKGFGFRPQDF
                     EKTCDVFSGGWKMRILLTSILLGRPDIMLLDEPTNHLDTESMEWLESYLKDYPGTILI
                     VSHDHFFLDKIATQIAELANRRIHIYKGNYTYYLAEKDRRFEALKKEMELQKAQIKKI
                     ESFVERFRYKATKASQVQSRVKMLEKFKDLQAEGAAKSVVMKFPEGKKSAKEVVTVCD
                     LGHAYGDLAVFSGLNFSLFRGDKVAFVGVNGSGKSTLSRLLSLFERPVRGSVTYGEGV
                     NMAFFSQESAQNLNYANTIWEEVQGVPTSADDQNKRNLLGAFLFSGDDIYKSVGVLSG
                     GEKSRLALLKIMLTDTNFLVLDEPTNHLDIKTKDVFQSALIHYSGTMAIVSHDRYFLD
                     RLVNRVFELKDGSVTDYAGNYSYFIEKRARMAADAGQQERRDRAPVDRTLKTKDKKRL
                     EAEERNRLSKIKNGLKRELAKLEEKIAELESRKAGDEHSLCDPQTHKDAVKIKGLQID
                     LKAVENELAQAYHLWTELSCKLEETDSDGFA"
     gene            complement(7865..8383)
                     /locus_tag="PLQ77_00640"
     CDS             complement(7865..8383)
                     /locus_tag="PLQ77_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL09218.1"
                     /translation="MDKRMNALAFALENEEKEREFYLAHARKTRNMAGKNMFKQIADE
                     EKEHYEMLKKLHAKWEEKKKWPATIALKVRPSAAGAILQGMSGKKSARIAGTDDELKA
                     VRTAIDFEARGVALYTKLAKQSTDAKEIAFFNMLASVERQHLLSLKDTEEFLADPATW
                     YQHMERSGLDGA"
     gene            complement(8470..9390)
                     /locus_tag="PLQ77_00645"
     CDS             complement(8470..9390)
                     /locus_tag="PLQ77_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPL09219.1"
                     /translation="MLDAIDRALLKTVAELEELPKGAYNIRKNGKLLDRHVSANINIE
                     TNEDETGIIVKIKPGTKNESVHIPVILSQAGLYDVVQNAFVIGDDAEVTIIAGCGIHC
                     GSSEPEGHAGIHEFKVGKNAKVTYVEKHYASGEGTGRRSLNPTTKVFLAEGARAEMEL
                     TQIGGVDDTHRVNEAVLGPNSLLLITERVLTDGEQTAVSTNDIVMKGRGSRANMISRS
                     VMKGNSKQTFYATIDAQNQCYGHIECDAIIMDNGTNETMPSLKAGHPDAELTHEASIG
                     KIANDQLTKLMSLGLTYDQAVNRIIQGFLK"
     gene            complement(9371..10129)
                     /locus_tag="PLQ77_00650"
     CDS             complement(9371..10129)
                     /locus_tag="PLQ77_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL09220.1"
                     /translation="MLELQKVYFTVQDTEGSDGGPRNIIDGIDYVFEDGKFYVITGPN
                     GSGKTTLAKLIMGVNPLSGGKILFNGQNISTLTITQRAKEGIAYSFQQPARFKGITFR
                     DLLTIATGIEEEPRLLEILGRVGICPLSFLDKEVDARLSGGEIKKIELATTIAANPKL
                     AIYDEPDTGIDLWTIGPMVSLLKKELAENRTTTIVVSHNKAFLEAADVVMIINSGRIV
                     YDGDLEGAMPLLDDLSVCTYRNSCEGERDAGCYR"
     gene            complement(10239..10433)
                     /locus_tag="PLQ77_00655"
     CDS             complement(10239..10433)
                     /locus_tag="PLQ77_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09221.1"
                     /translation="MMLRPCRSPGVKAPVPEVKGAVTGFGDVHRRAHLCRAAGEGPAY
                     AHRKAKERQEKRGGRKRNRR"
     gene            complement(10556..10726)
                     /locus_tag="PLQ77_00660"
     CDS             complement(10556..10726)
                     /locus_tag="PLQ77_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09222.1"
                     /translation="MAATAKKVVKKAAAKKPAAKKKVVKKVAAKKPAAKKKVAAKKPV
                     AKKKVVKKAAKK"
     gene            complement(10975..11757)
                     /locus_tag="PLQ77_00665"
     CDS             complement(10975..11757)
                     /locus_tag="PLQ77_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPL09223.1"
                     /translation="MRAKGKMKPILLRLAVLFMMGLLLQACAGREAKVYPGSHRPQKR
                     QLAEMGYTIQAGAFARVENAVRLTQRLKGQGLPATYFRADDGLFKVRFGNFSTKEPAR
                     LRAESLAQAGVIEEYYIVLPEDSPAAKRKQYGTDYLRESLVKTAHDFIGVPYLWGGVS
                     ADTGFDCSGLAMTVYQLNGLDLPRHSRTQFDAGDSIARQELQKGDLVFFDTRGGGKVS
                     HVGIYIGAGRFIHAPSSGKKIRIDSLASDYYARRFIGAKTYL"
     gene            complement(11765..13114)
                     /locus_tag="PLQ77_00670"
     CDS             complement(11765..13114)
                     /locus_tag="PLQ77_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPL09224.1"
                     /translation="MIRKADDHSIEKRLGAKLVVEGLRRAGFEAYFVGGCVRDFVRGV
                     SPGDYDIATSAPPDQVIRLFERTVAVGAKFGVVVVCEGGFSYEVATFRSDEAYEDGRR
                     PTQVRFSSAREDVLRRDFTINGLLMDPETNDIVDYVGGREDIGKKIIRTIGDPAVRFS
                     EDYLRMLRAIRFAAHLDFHLEDGVHEAMVRHTAKIREISAERVQEELNKILTRSGARR
                     GLELMADTGLLKHILPEVDRTRGVAQPPRFHPEGDVWRHTLIMMEILSNENRPEAEAA
                     LAWGALLHDVGKPVSRTEDEKGVHFYGHSVTGVSIAEDVMRRLRFSRALRETVLDLIR
                     QHMAFMNVQKMRPARLKRFLRMPNFHLHLQLHRLDCLASHKILDHYAFCVEQLQKTEP
                     EDLHPPRLLTGDDLLAMGFAPGKQMGEILRALEEEQLENRIHEKEEAVAFVQTRWKF"
     gene            complement(13140..14411)
                     /gene="thiC"
                     /locus_tag="PLQ77_00675"
     CDS             complement(13140..14411)
                     /gene="thiC"
                     /locus_tag="PLQ77_00675"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPL09225.1"
                     /translation="MTQLEKARKGVLTEEMKQSAAREGVSPEAIRQGIVDGTIVICRN
                     INHTAIEPLAIGLGLRTKVNANIGTSRDNQDLKLELEKLSVAIAAGADAVMDLSTGGN
                     LADIRKAVMAKSSVAIGTVPIYQAAVKTLEEKKAISEMTPDDLFGVIEENGRDGVDFI
                     TVHCGVNRESVAAVEAQGRVLGIVSRGGSMTANWMRCNDRENPLYEEYDRLLDIARRY
                     DMVLSLGDGLRPGAIHDATDQGQLMELIILGQLAARARAAGVQVMIEGPGHVPITEVE
                     ANIKLQKKLCQGAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYVTPAEHL
                     RLPTLADVRDGVMAARIAAHIADIAKGIPGAFERDRKMSECRRKFDWQGQVKASLDPD
                     KTSALLEKSKSANEEGCTMCGEFCAIRLGKK"
     gene            complement(14408..15232)
                     /gene="thiD"
                     /locus_tag="PLQ77_00680"
     CDS             complement(14408..15232)
                     /gene="thiD"
                     /locus_tag="PLQ77_00680"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPL09226.1"
                     /translation="MGKPIRILCIGGSDSGGGAGIQADLKAVHACGGYAVCAVTAVTV
                     QNTRGVQSVLPVPPKWIAAQLDSVLEDIGADAVKTGMLMTPEAVETVAGKIKQYRLKN
                     IVVDPVMIAKGGRSLMTDRARRALVKTLLPLADVLTPNIPEAEFLSRMKIRSVEDMKK
                     AAAVLSDLGVKRVVVKGGHLPGRKTRGCVDILYDGLRYDMFAAKWTQTKNTHGTGCTF
                     ASVLASCLAQGRGTQEAVRQAKRMTERAIQHSLSLGRGHGPVNLWGDFGVKKEKKS"
     gene            complement(15233..15904)
                     /locus_tag="PLQ77_00685"
     CDS             complement(15233..15904)
                     /locus_tag="PLQ77_00685"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPL09227.1"
                     /translation="MNRCIVIISGGRLGDMDFFRKKMSEAGSDLLIGCDGGARHLAAA
                     GVTPDVLVGDMDSIESSRLADYVRRGVKIIQHPADKDFTDTALALDYALGLKPERIQI
                     WGALGGRIDHALANLFLLVRAKEAGVAACLLDEYCEVFVPVDDAVFENRKGCLVSLLA
                     LSPRVEGVTLEGFAYPLEEGTFELSESRGVSNIIAAERARIRFRSGHLLVIRYRQKDF
                     FPEAD"
     regulatory      complement(15965..16076)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16108..16488)
                     /gene="queD"
                     /locus_tag="PLQ77_00690"
     CDS             complement(16108..16488)
                     /gene="queD"
                     /locus_tag="PLQ77_00690"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPL09228.1"
                     /translation="MMEIFKVFHFDAAHSLPRVPEGHKCAKMHGHSFRVEIHLRGKAG
                     SDSGWVTDFADIAAAFEPILEQLDHKNLNAVEGLENPTSENLCRWIWLRLKPVLPQLS
                     RIVLQESPESGCIYEGEDEPEAAI"
     gene            complement(16491..17129)
                     /gene="queE"
                     /locus_tag="PLQ77_00695"
     CDS             complement(16491..17129)
                     /gene="queE"
                     /locus_tag="PLQ77_00695"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HPL09229.1"
                     /translation="MYSIKEIFYSIQGEGYHTGRPAVFCRFAGCNLWSGREEDRSRSI
                     CKFCDTDFVGTDGINGGVYRSSAELAHKISLAWPVKKNKLTRPFVVCTGGEPLLQLDA
                     ALISALHKENFEIAVETNGTLPAPAGLDWICVSPKADAPLKQAAGDELKLVFPQEGAE
                     PKKYERLAFQHFFLQPMDSPHGGEAVRLSLAYVLAHPRWRISLQMHKVLGVR"
     gene            complement(17180..17902)
                     /gene="queC"
                     /locus_tag="PLQ77_00700"
     CDS             complement(17180..17902)
                     /gene="queC"
                     /locus_tag="PLQ77_00700"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPL09230.1"
                     /translation="MNDPSIKTRPRAVVLLSGGVDSATTLAIARNMKFDVHALTFRYG
                     QRHMVELEAAARVARENGVVEHRTVDIDLRCIGGSALTADIAVPKHGSVEELGKNIPM
                     TYVPARNTIFLSYALAWAEVIGSSNIFIGVNALDYSGYPDCRPEYIEAYERLANLATR
                     AGVEGTEKLRIHAPLIHWSKAQIVKRGMELGVDYSLTHSCYDPDADGASCGRCDSCLL
                     RLKGFKEAGIKDPGRYRLKTEG"
     gene            complement(17942..18718)
                     /locus_tag="PLQ77_00705"
     CDS             complement(17942..18718)
                     /locus_tag="PLQ77_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPL09231.1"
                     /translation="MLIDSHAHLEMKEFDPDRSDVIERARAAGVDCIVTVGTNPALSR
                     KALSIARRHENIYSTVGIHPHDAAAADDSSLDELKKLAQDPKVVAYGEIGLDYFRDLS
                     PREKQAEMFARQLDLAGELRLPVIIHDREAHEQTLRMVKASGIRSGVFHCFSGDWSMA
                     RQCLDLGFYLSVPGVVTFDRSKVLQEVVRQAPMDWLLLETDCPYLAPVPHRGKRNEPS
                     FLVHTARKVAQIKGLAWEDVAQTAARNACRLFCIPPPALK"
     gene            complement(18712..18888)
                     /locus_tag="PLQ77_00710"
     CDS             complement(18712..18888)
                     /locus_tag="PLQ77_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09232.1"
                     /translation="MSKRVNPELLKIDRRIIARKLLIGELSEKDLSSLLKKLPDVSEN
                     AEEIQIQPGEKPSC"
     gene            complement(19011..19823)
                     /locus_tag="PLQ77_00715"
     CDS             complement(19011..19823)
                     /locus_tag="PLQ77_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09233.1"
                     /translation="MFYKNKLALAGLAIVVLLFVISLAAPLISPCDPGAIDLQNVLEP
                     PSVEHWFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILIGMVLGAVAGYYGGWVDA
                     VIMRFVDVMLCFPAFFLILAVIAFLEPSIWNIMIVIGLTGWMGVTRLVRADFISLRER
                     DFVWAARAIGANDARIIFLHILPNALASILVAATLGIAGAILTESALSFLGIGVQPPT
                     PSWGNILTAGKDNIDIAWWLSLYPGLAILITVVGYNLLGEGIRDASDPRLKR"
     gene            complement(19848..20822)
                     /locus_tag="PLQ77_00720"
     CDS             complement(19848..20822)
                     /locus_tag="PLQ77_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09234.1"
                     /translation="MFPYLVKRILFMIPLLLGITIICFFVMRLAPGSPTDLQTQMNPK
                     ASAEMRQRLMSLYELDKPIHVQYVSWLTKLIRGDLGTSFSSDHRPVADKILERLPITI
                     TINLLSLVLIIAVAVPIGVLSAVHQDSLFDKVMSVIVFIGFAVPTFWLALLMMIFFGI
                     HLGWLPISGLRSLNYEYLSTWGQWTDLAKHLILPVFISAFGGLAGLSRYMRANMLEVI
                     RQDYILTARAKGLSERQVIYKHALRNALLPAITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTVMGILLIGAVLTLAGNLIADVSYAAADPRIRIS"
     gene            complement(20837..21634)
                     /locus_tag="PLQ77_00725"
     CDS             complement(20837..21634)
                     /locus_tag="PLQ77_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPL09235.1"
                     /translation="MPDMIRKVHVHMPYLLLGRHLPMVLAKKLNLEIYFSHDALQSLD
                     RNQCAKVSRTLENAGLKVTFHAPFMDLRPGALDDGIRRASLDRIRQVFDLIPIFKPLK
                     IVCHPSFDQRYYVSCDDLWLENSVNFWRQLIVQAEDGGAVIALENVYEREPGILRRLL
                     EALHSRQVCFCFDTGHFNVFARAPLEDWMEQLGRFIGHLHLHDNFGKFDEHLPVGGAT
                     FPFGLFFAALKKLDVRSTVTLEAHAEEHLWQSLANLRNSSYLDGLNL"
     gene            22015..22410
                     /gene="rsfS"
                     /locus_tag="PLQ77_00730"
     CDS             22015..22410
                     /gene="rsfS"
                     /locus_tag="PLQ77_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPL09236.1"
                     /translation="MPVKKNESARRLLLCINAVLQKKAKNIVVLNVREISSFTDYMLI
                     CSGTTDRQVQAVASTVQEYLKKEGIRPLGVEGKANAEWILLDYDDVVISIFQEAARSF
                     YDLENLWDAPRMEIDENATEIKKLHKDMT"
     gene            22407..23135
                     /locus_tag="PLQ77_00735"
     CDS             22407..23135
                     /locus_tag="PLQ77_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPL09237.1"
                     /translation="MILYEFLSQISDAFFPRICLACSRILPSGRPEFFCSDCFGQITF
                     LHGSLCPVCGILFPDSSSEDHLCPSCMENAHWFEFARAAVAYEGIIADVIRRFKYGRD
                     LTAGEALAGLLADFDFDGVDFSAFDAIIPVPLHIRRLRERRFNQSLVLARALGKKHGI
                     AVDFSLLKRRRFTLTQTGLDKSERDKNISGAFRASHSEKIPGGSFLLVDDVYTTGATI
                     NECAKTLAEAGAKRVAALTAARVL"
     gene            23294..23779
                     /gene="rfaE2"
                     /locus_tag="PLQ77_00740"
     CDS             23294..23779
                     /gene="rfaE2"
                     /locus_tag="PLQ77_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPL09238.1"
                     /translation="MNKILNRTELKEKLDALRQEGKKIAFTNGCFDLLHVGHVRYLKE
                     AGKTADVLVLALNSDSSVRAIKGLKRPLVPEQERAEILAELECIDFITIFSEPTPLEL
                     ICLLKPDVLIKGGDWAEEQVVGRDEIRRWGGRVEIIAEVAGKSTTNIVDKVMAVYGPD
                     K"
     gene            23913..24293
                     /gene="dksA"
                     /locus_tag="PLQ77_00745"
     CDS             23913..24293
                     /gene="dksA"
                     /locus_tag="PLQ77_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HPL09239.1"
                     /translation="MEKQTTLKPEKLEAFRKLLTQKINELLSEAGKTVSEMTSGKENF
                     PDPNDRASLESDRNFELRIRDRERKLIVKMQEAIKRIDDGVYGICESCGGPISEKRLL
                     ARPVTTECIDCKTKQEKMEKLKGE"
     gene            24382..26790
                     /gene="priA"
                     /locus_tag="PLQ77_00750"
     CDS             24382..26790
                     /gene="priA"
                     /locus_tag="PLQ77_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPL09240.1"
                     /translation="MFISVAVNIPSDKLFTYEVPVHLQNAVEVGKRVFVPFGIRKRSG
                     FIVEILKNCDLKDTKKIVAVLDEEALFDAEDLKFYRWISGYFLYPLGKTLAELIPSGP
                     ERRELRWIIPAPAARDARPTAAQKKLLDILHEHSGGLAFQDWIRKSNMKNAASIARSL
                     QVAGWVHVMEKEKKQLGIRMKKIIRLADELSGPMKLTPRQQIVADCLRREGPMELRAL
                     AEKTAASAAVIKRLFEKGAVEVREEEHIRRAPIESALAAPRQNIALNDEQKKALETIG
                     QFIDSEVFHPVLLHGVTGSGKTEVYLCAIDRVLKSGGSAIYLVPEISLTPQLISRITG
                     RFDPDRIAVLHSGIAESVRYDQWRQIRRGLIHLVIGARSALFAPLPNLKIIIVDEEHD
                     PSYKQDERLCYNARDLAVVKARSANAVCVLGSATPAVRTFFNARGGKYAHLELARRVN
                     RQPLPQIDIVDMKTQRETGGKSPVLSEALIAALRNVLDRREQALLFLNKRGFDTFLVC
                     ADCGYTFSCPNCAVSLKSHPAENLVKCHYCDYSRRAVPLCPRCGGSRILNYGAGTQKL
                     EAELQSLFPLARISRMDSDTTARRGSQEKILASLERGEIDILVGTQMVSKGHDFPSIT
                     LVGVVSADTSLNMPDFRAAEKTFQMLTQVAGRGGRGEATGRVIIQTFNPLHYALRHAS
                     AHDYLSFYEEEIEFRKALKYPPFAQIINLRLSSARKAALDETARELGRDARALCAGHE
                     NILEIIGPAESPLAKIRGEHRQQMMIKGRDNRRMHSMAAKLLKKHATSTVKITADVDP
                     ENFM"
     gene            complement(26869..27327)
                     /locus_tag="PLQ77_00755"
     CDS             complement(26869..27327)
                     /locus_tag="PLQ77_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09241.1"
                     /translation="MVESIKLLDVLEANIDEIAVHWAADVRKNKRTVHYKDIPEEKLV
                     LQAVKFYSQLRNMLMAPNRLDKAQEFFMHYARTCYEMGIPLHESIYALNMMRRHMWLY
                     AEFQAIFINAIEHNQAIDSVMRIMLLIDYAVYEITQYYQDRIRLETKQQF"
     gene            complement(27375..27833)
                     /locus_tag="PLQ77_00760"
     CDS             complement(27375..27833)
                     /locus_tag="PLQ77_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09242.1"
                     /translation="MKTYAIKYVELAEQHAGRMAKRWAMDVQNNARTKRYKELDEESI
                     VNQGVKFYHNFSKMFAEDKISEATMKYFRTYAQESFAMRIPMDEAVYALILLRRHTWL
                     YAEFQTIFSSGIDQRQALDTLSRTILLFDYAIYEVTKEYQELMKKGKGKK"
     gene            28011..28952
                     /gene="fmt"
                     /locus_tag="PLQ77_00765"
     CDS             28011..28952
                     /gene="fmt"
                     /locus_tag="PLQ77_00765"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPL09243.1"
                     /translation="MTKKPRILFMGTPAFALPALETLCQRGFPISAVVTQPDRPAGRG
                     KKDAAPPVKQMAQKFGLTVLQPASVKDPSFLEALDAVRPDMAVVAAFGQILPKAVLDF
                     PPQGCLNIHPSLLPAYRGAAPIHWSIIRGEKQTGVTIMLMDEGMDSGDILAQEKTPVG
                     ETETYGELHDRLALLGAALLVETIERLASGAVQRQKQDSSRVTFAPRLSRETGRICWR
                     NPAEHIINLVRGLNPSPAAYTLLEGQDLKILAAEARPGPVEEPPGAVAAATPEGLPVA
                     ASNGHVILKEVQLAGKKRMPVSDFLRGFPLKPGIRLE"
     gene            complement(28970..29347)
                     /locus_tag="PLQ77_00770"
     CDS             complement(28970..29347)
                     /locus_tag="PLQ77_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPL09244.1"
                     /translation="MFQIVKYETFNDRQVIFKEGTFGDWMYVIEEGAVEISRVVEGKK
                     IVIAVLKTGEIIGEVAYISKEGRSATATAVGKTRIGMIDREYFDREFNGISSDFQMIL
                     KSMARRLRETTDHLIQLQNQAGK"
     gene            29684..31585
                     /locus_tag="PLQ77_00775"
     CDS             29684..31585
                     /locus_tag="PLQ77_00775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL09245.1"
                     /translation="MPAGREDHLEKFKKRYPEKFVPEKDLFKCIRRGNSIFIGTACGE
                     PQNLLGSLIRYVENTPHTIYGTQIIHIWTLGVAPYANKKFKINFRHNSFFISDSSRQM
                     VNEGLADYTPTFLSEIPSLFRRGIITIDVALVQLSEPDEEGLMSLGVSVDIVKSAVEQ
                     ARLVIAQVNRHMPRVRGDAMVDIRDVDFILPHDEPILEYTPEPPGEVTEKIGTYVSRI
                     VQDGDTIQVGYGTMPNAILAHLSGKRDLGVHTELLTDGIIDLMKKGVVTNAKKSLDRK
                     KTVASFCMGNRDTYKYLNDNPAFEFRPIDYTNSLFVIGRQTNMTAINSALEIDLTGQS
                     TAESIGGIFYSGVGGQADFMRGSALAPGGKSILAMRSTSIDMAHSRIVPCLSAGASAT
                     LIRGDVHYVVTEYGTAYLHGKNIRERAMALISIAHPRFRPWLVEQARERGLIYRDQAF
                     IPGTTGVYPEHLETYRSTRTGLPLFLRPVKISDEGIIKAFLYSLSDKSIYTRFFTNMV
                     HMPHNILQKYVIIDYSKEMLMLAIQERQGMEEIVGMGQYIIDTDTHTAAASFLVGDVW
                     QNRGIGIVLLEYLTEIAKRDGLLGFSATVLAENKAMLRLFERMNFQMEKQFSSGVYEL
                     TMGFYPREP"
     gene            31702..32799
                     /locus_tag="PLQ77_00780"
     CDS             31702..32799
                     /locus_tag="PLQ77_00780"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPL09246.1"
                     /translation="MKKVLFMMVLLLVLFAGYFLFWPVPIEPVAWNAPAAAGYTGPHA
                     FNTGLSRLNLIDLGAEEGPEHIVLGKDGKLYVSVGSGNVLRMNPDGSGQEVFIRTGGR
                     VLGFDFDKAGNLIAADAIRGLLSVSPQGKITLLTRSVNGDPIRYADAVVVARSGMIYL
                     SDASRRFSPAEWGGPFEASILDIMEMSSTGRVLAYDPAQKSTRVVASGFCFANGLALS
                     RDEQNLFVNETGKYRLWKVSVHALELDIARPGDQAKIVFDNLPGFPDNLMRGQDGKIW
                     MGFAKPRNPDLDQMGSKPFLRKITLRLPRSLWPVPKAYGHVFAFTEDGRVVADLQDPG
                     GAYPETTGVTETADRLYIQSLHAKGLGWLPK"
     gene            complement(32838..33530)
                     /locus_tag="PLQ77_00785"
     CDS             complement(32838..33530)
                     /locus_tag="PLQ77_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPL09247.1"
                     /translation="MMNKVRNVLTQSQLFGGLPDEHLAEIENIAVPRRCGKGEMVFHD
                     GDEGIGFYLVVAGIVNVYKLSSEGKEQILHIVGEGDTIGAVPVFSGESFPANARAVTP
                     CHLLFFNREKFIRLITRQPSLTMNILALLSARLREFTVQVENLSLKEIPGRLASYLLD
                     LAREQGNRDRIALNISKLQLAGLLGTGPESLSRALGSLKSKKLIEEKGSMIRLVNRAL
                     LEELAEKGKNTP"
     gene            33644..35278
                     /gene="hcp"
                     /locus_tag="PLQ77_00790"
                     /gene_synonym="priS"
     CDS             33644..35278
                     /gene="hcp"
                     /locus_tag="PLQ77_00790"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPL09248.1"
                     /translation="MFCYQCQETVKNEGCTVKGVCGKTEETSNLQDLLIYLLNGMAVC
                     ADKAKEKGPLDPKYGRFMMEALFATITNANFDSDRIIGMIREALRLRSELRQACGAAF
                     PAGLHDCAVWDSDNMEEFKKKAAAVGVLSTENEDVRSLRMLLLFGIKGIAAYAEHAAV
                     LGFEDGKIHDFVREALISITEDLSVDEMVAMVLRAGECAVSAMALLDKANTSTYGHPE
                     ISKVNIGVRGNPGILISGHDLKDLDELLKQTEGTGVDVYTHGEMLPANSYPAFKKYAH
                     FVGNYGNAWWKQNEEFESFNGPILMTTNCIIPVRDSYKDRIFTTGVVGYPGLKHIPDR
                     SKGGTKDFSEIIELAKKCAPPRELEKGELVAGFAHHQVLALADQVVAAVKSGAVKRFI
                     VMAGCDGRHKSRGYFTDVARELPEDTIILTAGCAKYRYNKLALGDIGGIPRVLDAGQC
                     NDCYSLALIALKLKEVFGLKDINELPISFDIAWYEQKAVAVLLALLYLGVKGIRLGPT
                     LPAFASPNVLKVLVEKFNIKPITDSKTDVEAMVEGR"
     gene            35388..36122
                     /locus_tag="PLQ77_00795"
     CDS             35388..36122
                     /locus_tag="PLQ77_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL09249.1"
                     /translation="MKKILRKIIEIDEALCDGCGQCVTGCDEGALQIIDGKAKVVSET
                     FCDGLGACIGQCPTGALRIVEREASPFDEEAVQKHLAGQSQPPLPCGCPSAQIQILGK
                     PSACEAANQPKHLEEGEEESALTHWPIQIKLVPASAPFLKGADLLVLADCTAVAFPLL
                     HRNLLKGRVVMMGCPKFDDVQEYVQKFADIFQTANIKSVTVAVMEVPCCSGMPVIVRK
                     GMEAAKRIIPMTEIVLSLRGKILKQS"
     gene            complement(36301..38559)
                     /locus_tag="PLQ77_00800"
     CDS             complement(36301..38559)
                     /locus_tag="PLQ77_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL09250.1"
                     /translation="MKPETIDPSGHKSKNRIWLFSLCVIAFLIAASDLQAGPAVPDGR
                     PRIGLVLSGGGARGLAHVGVIEVLEEMKIPIDCIAGTSMGAIVGGIYASGMSPDELKK
                     FITSMQWNEAFKDKPDPSEMTFRRKRDAADFLIDLDLGFKDGEFAMPRGLLQGQNLNL
                     ILKSLLIHTEGIDDFNRLNIPFRAVATDIETGDAVILEKGELVKAIRASMSIPALFAP
                     VEIDGKLLVDGGVANNLPIDIARQMGADVVIVVDISTELSRRDQLDTSVGITSQLTSI
                     MVQRNTQAQIRTMREGDILIRPELGTIGSSDFFKAADAVAAGRKKAAEMKTELAHLAV
                     REEDFPAYLRAQRKKDQAMPVIDVVEVDNKSSFPDGLIAAQVNIKPGEPLNMPELKKN
                     IDRVYGIDTFERVDFHLRKKDKSTAIVIEPFDKAWGPNYFRFGLGLEDNFKGSGSYAL
                     TAQFTKTALNRLAGEWRTELRIGENPRIYTELYQPLDYSLSYFAAANAAYLMRNINTY
                     NDKGEILTQYRVQSAQFGVDAGRQFGNWGQVRAGLRREHGNVKIAIGDPSVPEETYNR
                     GSVQASVAYSRLDNYVFPLRGTDASLMWNYDLKALGSDIDEQALGFNWMTAFTWGKYT
                     FIPSLDVKTMLDDDSVAVQDTFPLGGFLNLSGFSTNEIYGRHTGLARLVAYREIGSAG
                     MGALKMPLYIGLSAEAGNVWNKRSDINFESLILAGSIFLGIKTYLGPVYLAYGQAQRG
                     HSSIYLNVGQRF"
     gene            complement(38585..39238)
                     /locus_tag="PLQ77_00805"
     CDS             complement(38585..39238)
                     /locus_tag="PLQ77_00805"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPL09251.1"
                     /translation="MLKVNKIDLRSSAFSLKIYTRCLMFYQIIKAGNDEIGLVWEDAG
                     GKTQVECIYLPGREKIIQKIFRDFPAVEKKPRKISGGLDRLIVDLCLGKERRVDLSFL
                     NLSGLTEFSGRVLRQTHRIPRGKVCTYSGLAARVGFPRAARAAGSVMANNPFPLVIPC
                     HRVIRADGTPGRFGGGADMKRRLLEREGIFFDAKGRVPSEYRVTAKRRRRDDAGDHS"
     gene            complement(39326..39883)
                     /locus_tag="PLQ77_00810"
     CDS             complement(39326..39883)
                     /locus_tag="PLQ77_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPL09252.1"
                     /translation="MSLITIFLLAVGLGLDAFSVAIAIGAANAKKSWAPVLRLSLAFG
                     LFQFFMPLAGWLAGSTVVDRIQAFDHWIAFALLVLVGGKMIREGFEKEKDEEKADQTL
                     GWPLFLLSIATSIDALAVGFSFSLLKTPILFPAAIIGMVCFVMTAVGMVFGKGLARIF
                     GRKVEILGGLVLIAIGVKILIEHMG"
     gene            complement(39911..41758)
                     /gene="uvrC"
                     /locus_tag="PLQ77_00815"
     CDS             complement(39911..41758)
                     /gene="uvrC"
                     /locus_tag="PLQ77_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPL09253.1"
                     /translation="MDENAAVKEKMASAPRAPGIYIMLDARRKVIYVGKANDLKSRVG
                     SYLTGRDTRPMAPFLMARVQSIEFITTDTEKEALILENNLIKKHRPRYNVILRDDKTY
                     CHLSLNPSEAFPRLRLVRKRQNDAALYFGPYPSGLAARETLRFIQRIFPLRTCRDRDF
                     QLRARPCLEFQIGRCLAPCKNRIDEAAYRTLVENAVSFLRGRRRELISDLKKQMSEAA
                     QSLRYEDAARLRDRIGALEHALERQHVDAGGAADQDVLGVHSDSGTHRVCILFVREGK
                     LLGSKSFTPVKTKADREEILSSALRQYYDEAGLPEEILLPFSLSDEAVLLEWLAEKRQ
                     RSVRITVPQRGAKKALVDMACANARELQASDRKKDEQKTAVLQFLRERLSLAGLPRRI
                     ECFDISNLGGKNAVGSMVVFQDGEPDKSAYRRFRVRGPDEPDDYGMMREVLTRRFQGR
                     DPVPDLLVVDGGKGQLNVALAVLSDLKIKLDVIGLAKEERSFLAAKRRLQGKPAKSED
                     RVYLPRRKDAIYLSSSPAALSLLQRLRDEAHRFAVSYHRKLKQKNDFRSALDDIPRLG
                     SRRKSILLRHFGSLERIRSCSPEELQALPGIGRALAETIHKVLSSGGAA"
     gene            complement(41779..42615)
                     /locus_tag="PLQ77_00820"
     CDS             complement(41779..42615)
                     /locus_tag="PLQ77_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPL09254.1"
                     /translation="MAIPYHALTYPDDRRTSREAMGCPAFAVIAHRGASACCPENTLS
                     AFRAAIEMKADMVELDVQLTADREVVVFHDAKLSRLTDGRGMVADYPFARLRQLDAGV
                     WFHKKYAGERIPALVEVLALCKGRMAVNIEVKTEAVTDAVSGGIEERCLKMVDQIGMK
                     EHVVYSSFDPRAILHFKQIDREAPVAVLYEKKQHGSRLPSEIVLSLGVDAFNCSRREL
                     NGTWLADLKRHGIPVHIYTVNDEKNMRRLLEMGVDGIFTNRPDVLRNVCEEYKGRTRD
                     EG"
     gene            42767..43342
                     /locus_tag="PLQ77_00825"
     CDS             42767..43342
                     /locus_tag="PLQ77_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPL09255.1"
                     /translation="MKVLGIMGSARVGGNSDVLLSRALEGAREAGARVQKIVLDRKHI
                     AGCRDCKKCNKTGICVLADDMSGIQELILEADAVLHAVPVYFWSMSSQMKAYLDRWCV
                     FFDAEWRWQKACYPKMRGKRIGLLTVCGDPDPHTADPIVHSFRMTAEMTKMHWLGAVM
                     ASASDKGEIKGNGAALREAFDLGKKSAAPQQ"
     gene            complement(43471..44520)
                     /locus_tag="PLQ77_00830"
     CDS             complement(43471..44520)
                     /locus_tag="PLQ77_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL09256.1"
                     /translation="MKKSSASLPVHPKKKKLNLALQGGGSHGAFTWGVLDRLLDEKRL
                     EIEGIVGTSAGAVNATILAYGLAAGGPAKAKELLNRFWMTNSKNSRCSPFQPTVFDKL
                     MGSKGSIEFSPYWQMFDILSRMFSPYQWNPNNKNALKEMLEELIDFSVLQKSRSIRLF
                     ICATNVLTGRLKVFENREITPDMIMASACLPYLTQAPEIDGQYYWDGGYMGNPPIFPV
                     IYDTDCRDVLIVQINPINIPEVPRSTNEIFDRINTLSFNSSLMREMRAIYFITSLIEK
                     GELDSAKHKHTFIHTIDAEEEMAKLSASSKMNLDVEFLQYLFDTGREKADEFLKNHFD
                     KIGRRSSTDLVAKFF"
     gene            complement(44763..45368)
                     /locus_tag="PLQ77_00835"
     CDS             complement(44763..45368)
                     /locus_tag="PLQ77_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09257.1"
                     /translation="MTKRLSGILVMIVVMTFHSAVSSALDKAQKFKEYASFGNYFQCS
                     IPEDWSVYPPGFGLSEEEKKVYGVILIGPRNTSPVAPLMSIHYYAPGNLLHSTMDIFI
                     RRHSRSNLGIEIEGKSYGKIQEMEVAGRQAKTFERIDIRFIGERVINPTKVPIYEKFV
                     TIPAGSGEGFYVLKLSVPAEMKDAYKGLFEVTVKSFLPKNE"
     gene            complement(45477..47066)
                     /locus_tag="PLQ77_00840"
     CDS             complement(45477..47066)
                     /locus_tag="PLQ77_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL09258.1"
                     /translation="MKHAVKIMALFLCFFVPLMGQVAFAETRTFIKEYTYQAGDEDSK
                     NSSRVIALREVKRLLLEELGTYLESETEVKNFQLTKDQITTLTAGIVQTEIIAEKWDG
                     HVYWLKSKIVADSDKVVQSIDAMRKDRDKTKELESMKQKSDELLREVERLRKAMASAK
                     NGGRESQKAAYDQSIRELSAADWIEKGHAVSGPEDNFKGAFDAYSRAIELDPKNIEAY
                     YFRARISEKNQAMSDYYKILSIEPKDSEAHLIRAWTYKELDQLDPALQEFGKAIAKAR
                     GNKEKAVAYFDRGRYYTLFRSRPYVAPSKQDIPGALELSVRDFSSAIALDPADSSYFH
                     NRANSYLGLNQYDLAIEDFNAAIRLDPKSAGLFSGRGQTYQLLQKPELAVADLSRAIE
                     LEGPDHLFAAHDYMLRAMSYEKLGKFDLAIQDWTNLLKRKPDDPFYLRERADLYSKIG
                     KYDQAIKDYGRSIALNPREAAAAYYGRALAYALKGDSRKSIQDLRNAIQLDPGYKANV
                     RNEAGFNSLQHHPDFIKLIGK"
     gene            complement(47110..49767)
                     /gene="polA"
                     /locus_tag="PLQ77_00845"
     CDS             complement(47110..49767)
                     /gene="polA"
                     /locus_tag="PLQ77_00845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPL09259.1"
                     /translation="MAEKNQTPLLAIVDGSNYLYRAYYGIPALTNSKGFPTNAIYGFT
                     TMLMKLLRDLSPDYIVVTFDLKGPTTRHGEFEDYKATRKPMPDDLIPQVPFIKDVIRG
                     FSICVLEKQGTEADDLIGTLAVAARQKGWRTVMISGDKDLMQLIDDRVTMVDTMKDKT
                     YDAAAVEEKFGVGPEKVVEILGLTGDTSDNIPGVPGIGPKTARRLIEEYGSVDAVIEN
                     AQKLHNVKLRESFRNYADQARLSRQLALIRTDIEMDFHLEDAARRKPDLETLARLFGE
                     FEFSSLLQQIKIKAEKPVKACKVVADGKSLSELIAGLHNNSKIACDMIREKNPPQELI
                     GMAFRAGGDSFYLPLGHTPAKEQLDPKETFSMLASFFANAEIVKYAYDLKTTLVSMPD
                     LTIRAGFDLQLAAYLLNPARHSYALDEMAWEYLHERISTPDDIAGGKGKTYALSLAPV
                     DKMAAYAGERAEAIMALAEVLESRLEEAGAASLYRDVEMPLLFTLAGMEKKGVLVDTV
                     LLQQMSDELGQLLSISEDKIHRLAGEKFNINSPKQLQTILFDKLKLPKGKKIKEGYST
                     DVDVLTDLARSHELPAEILAYRSLSKLKSTYMDALPVLVNPRTGRIHTSYNQTVAATG
                     RLSSSNPNLQNIPIRTMEGKRIRQAFIAAPDCVLISADYSQIELRVLAHLSEDATLLE
                     AFAADEDIHTRTASDIFGVFPEMVNPDMRRQAKVINFGVLYGMSAFGLARELGISQKT
                     AQAYIDGYFARYKKVRDYLDGILEGARRDGFVCTILNRRRYLPELQSRIPSVRQFAER
                     MAINAPIQGSAADLIKVAMVNIDRLLTEKNLSARLIMQVHDELVVEAPVEEKEDVTVL
                     VKKEMEGVMKLKVPLKVEIAAGSNWDEAH"
     gene            complement(49780..50226)
                     /locus_tag="PLQ77_00850"
     CDS             complement(49780..50226)
                     /locus_tag="PLQ77_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPL09260.1"
                     /translation="MDAIDKKLLNLLQKEFPLEEEPFRLIGERCGISEDEVLARVRRM
                     KEKGVIRRIGAVFDGKKLGRASTLCAARVPEDKLQSFVHIVSAEKGVTHNYRRGHEYN
                     VWFTVTAANTEELQALLSDVKKKTGVTDMLDMRAVRTFKIDASFDL"
     gene            complement(50359..51459)
                     /gene="ahbD"
                     /locus_tag="PLQ77_00855"
     CDS             complement(50359..51459)
                     /gene="ahbD"
                     /locus_tag="PLQ77_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HPL09261.1"
                     /translation="MSRPFPKGYASHEGKGNSVRMIAWEVTRSCNLNCVHCRAASHLG
                     PYPGELSTDECLRLVDDIAGVSQPVIILTGGEPLLRPDIFDIAAYGTRKGLRMVMATN
                     GTLVDQAVARKMMESGIQRVSVSIDGQDAESHDAFRNEAGAFAGAMRGIEAMKSAGME
                     FQINTTITTANLRQIKAIHDLVLRVGAAAHHIFLLVPTGRGKDLAQQAITAADYEETL
                     HWFYRESLTCEIQLKATCAPHYFRIMHQNKPKGAKPVKNAGGHFHESTRGCLGGITFC
                     FISHVGQVQPCGYLELDCGNVRKQSFGEIWESSEVFRNLRDYGRYKGKCGRCEFIKVC
                     GGCRARAYEATGDYLAEEPLCLYEPGALKTGF"
     gene            complement(52111..53085)
                     /gene="hemB"
                     /locus_tag="PLQ77_00860"
     CDS             complement(52111..53085)
                     /gene="hemB"
                     /locus_tag="PLQ77_00860"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPL09262.1"
                     /translation="MQFPEYRGRRLRKNENFRRLVRETRLSADELVYPLFAVPGRDVK
                     KPIASMPGQFQMSVDHLAKEAREAHALGIPALLLFGIPARKDEQGTGAFAKDGIVQQA
                     VKRVKNEVPDMLVITDVCLCEYTSHGHCGIIEKGQVQNDATLEVLAETALSQVKAGAD
                     MVAPSAMMDGQIAAIREILDENALENIPIMAYSAKYASSFYGPFREAAESAPQFGDRK
                     AYQMDPANSDEAIREISLDVNEGADIIMVKPALPYLDIICRAKQEFDLPLAAYNVSGE
                     YSIIKAAAKLGWLDEGKAMMESVTAIKRAGADIIITYWAKDLAKALAK"
     gene            complement(53089..54276)
                     /gene="ahbC"
                     /locus_tag="PLQ77_00865"
     CDS             complement(53089..54276)
                     /gene="ahbC"
                     /locus_tag="PLQ77_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HPL09263.1"
                     /translation="MIGISKLYCGAVEPSDVLRYNRHSGSLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCIHCYSNSADIDYPDELTTQEAKKMIDDLAAFGSPVLLFSGGEPLMRKD
                     LLELAKYATDKGMRAVISTNGTLITKDIAAELKKIGLSYVGVSLDGLQPTHDRFRGRK
                     GAFEKAIEGIRNCREAGIKVGLRFTVNRHNVADVPAMFDLLRKEKIERMCFYHLVYSG
                     RGSKLREEDLTHGEARRLLDLIADKTKAMFDDGLAPEILTVDNHADGPYLYLKMREKD
                     PARAAEILELLEMNEGNSTGVGIGCISWDGEVYPDQFWRNRPLGNVRKRPFSEIWTDE
                     KNEFLMKLKDKKKYVTGRCAACVWLSVCGGNFRARAEAEGDEWGPDPACYLTDEEISS
                     PKE"
     gene            complement(54273..55805)
                     /gene="cobA"
                     /locus_tag="PLQ77_00870"
     CDS             complement(54273..55805)
                     /gene="cobA"
                     /locus_tag="PLQ77_00870"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPL09264.1"
                     /translation="MKKGKVYIIGAGPGDPGLITLRAVEALKLADAVVYDNLVNEELL
                     AYAPVGARRIYAGKQGGDHTLTQDKINDLLVREALDGRTVARLKGGDPFIFGRGGEEA
                     EELAASGIPFEVIPGVTSAIAVPAYAGIPLTHRGLTSTVAFITGHEDPTKEKSDIDWQ
                     ALAGIGTLVFLMGVKNLGQITDALILRGKSPETPAALIRRGTLPEQQTLTGTLANIEE
                     LARANQFKPPAILVVGKVVELRDTLGWFDSKPLFGLGVVVTRPERQADDLAKLLAAEG
                     ARPIAFPTIAIEPPSNWSELDAALEELESYQWIIFTSANGVHFFFERLRQKGKDMRDL
                     KGVKVCCIGPATARQLEARGIGVDLVPEEFIAEGILKSFAALNIQGQKILIPRAAKAR
                     DLLPEGLKKMGAEVVVATTYRTVNSGRKKEELAVRLAAGDVDVITFTSSSTVTNFVDI
                     MGKDFALPPSVRVACIGPVTAGTAAKAGFKIDILQNEYTMEGLVRALVDHFKHPEGKD
                     SP"
     gene            complement(55807..56721)
                     /gene="hemC"
                     /locus_tag="PLQ77_00875"
     CDS             complement(55807..56721)
                     /gene="hemC"
                     /locus_tag="PLQ77_00875"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPL09265.1"
                     /translation="MNIKIGTRGSKLALVQTNSAVAALQKAAPEIEAEICVIRTSGDI
                     MQDVSLTQIGGQGVFVKEIEEALLAGRIDLAIHSMKDVPGETPEGLVFAAILPREDVR
                     DVLVTRGRIKFEYLPKGAKIGTGSQRRGAQLKSFMPDLEIVPLRGNIDTRLKKIETEN
                     LTGVVLAAAGVKRLGYGEVVTQFLPTEIMLPAVGQGALGLQVRKSDAELAGLCARLNH
                     AVTEAEVTAERAYLRALGGGCRLPIAAYGLMEGKRLTLEGLLAAPNGGEVLRDKVWGE
                     PGEAEAMGRKLAEMILEKGGRRLLELMG"
     gene            complement(56903..>57311)
                     /locus_tag="PLQ77_00880"
     CDS             complement(56903..>57311)
                     /locus_tag="PLQ77_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09266.1"
                     /translation="PAADDVENVYLYNIDHLQNIVDENIKNRRREALKAEAIIEEEAA
                     NYVSWLKELEAVPTIVSLRSKAENIVRAEMEKASVWMQSLENADREKVEALVHGIVNK
                     VLHAPVAVMKEESAEFASQDIVAAARQLFRLDD"
BASE COUNT        12582 a        16843 c        15234 g        12652 t
ORIGIN      
        1 agcggaatgt tgaaggcgtc gcagaaacgg atgaagcgcg acgccttgtc cgaagcgttg
       61 acgttgagaa cacccgcata agacatggga ttgttggcta tgactcccac gggccggccc
      121 atgatgcgga tcaaagcgac gatgatgttc ggcgcccact gcgcgtggag ctcaaaccat
      181 tcgccgttgt cggcaaccga tttgatgatg cttttcatat tgtaggcgcg cgccgctctg
      241 tcgggaatga tcttgttcaa ttccgggcac tcccggtccg ggttgtcggt cgtctctgcg
      301 accggcagcg gcgaatgaca gctgtccggc aggtacgaca gcaatccttt gattttttga
      361 atgcagtctt cgtcgttttc agtcacgaca tggcacacgc cgcttttgga ggcgtgagcc
      421 acggctccgc ccaggtcttc ctgcgaaact tcttctccaa tcacggcctt gatcacgtcc
      481 ggccccgtga tgtacatgta cgatgtattc ttcaccatga aaatccagtc ggtgagcgcc
      541 ggagaataca cggcgccgcc ggccgtgggt cccatgatcg ccgtgatctg gggaatataa
      601 ccggaggcca gcgtgttgcg gtagaagatg ccgccgtagc cttccagaga cggcacgccc
      661 tcctgaatgc gcgccccgcc ggaatcgttg atggaaacaa acggcttttg cgcgtccagc
      721 gccatgtcca taactttcca tatctttttg gcgtgcattt cgcccagggt tccgcccgcg
      781 ctggtaaaat cctgggcggc ggcgaagaca atccggccgt tgactttgcc gaagcccgtg
      841 atgacgccgt cggccggaat ttcctttttg tccatgccga acagggtggc gtgatgcttc
      901 acgaaaagct gcacttcctg aaagctgcct tcatcaaaca accggttcaa ccgttcacgg
      961 gccgtgagct tcccgccgtc gtgctgcttt tgcacctgct tttccccgcc catcttcatc
     1021 atcgactggg cccgggtttc aaagtcccga attttccctt ccgtcgtcgc ctgcctgata
     1081 tcgtccccca tgactgatct ccttcgtaat ttcctgattc gataaaccgt tttccgtttt
     1141 atcttgaccg ggcttcgggc ctgcgttact atcccggacg aaaaaatacc ggcccggcca
     1201 tgtgttttga aaacggcgaa gaagtgctta acctttttgg ggcaaactgg caagtctgat
     1261 ttgtgcgata tgcgcaactt caaaatgatt ctcgaatatg acggcgcagc ctactgcggc
     1321 tggcaaaggc agcaaaacgg gctttccatt cagcaggtag tggaagaggc gctggcgcgc
     1381 atcatcggcg aaaacgcgac ggtcatcgcc tccggacgaa cggacgcggg cgtgcatgcc
     1441 atgaatcagg cggccagctt tcggtcgcgc accgttctcc cggccaacaa aatttttttg
     1501 ggcgtcaaca gcgttttgcc ggacaacatt gtcgtgaagg atttgcagga agcgccgccg
     1561 gatttcaacg cactgaaaga tgcgagaggc aaggtttatg tgtatcgcat acaaaatcag
     1621 cgtctgcgcc ccgcgctggg acgccagtat ttctggcacg tgcgctgccc gctgaacctc
     1681 gagagcatgc gcctggccgc gatgaaattg atcggtgaac acgatttttc gtgtttttgc
     1741 gcgacgggct gtgatattaa gaatcgcgtc aggcttctca cggtctgctc gattgtccat
     1801 caggaaagcg gacggctgga aatcacgctt gaatcgagcg gatttttgcg ccatatggtg
     1861 cgcaacatcg tcggcacgct ggtggacgtg ggccggggaa aaatcgcgcc ggaaagcatg
     1921 ccgcaaatcc tcgcctcgcg cgaccggaaa caggccggaa cagccgcgcc cgcctgcggc
     1981 cttttcttaa aggaggtgaa atactgaaca catgaaagta ctgctgctgg gacacaaggg
     2041 aatgctggga aacgatctga tggcgcaatt cagacaccgg cacgacgtca tcggcatgga
     2101 cagggaggaa atcgacatca cccgcgcctc cgaatgccgc agggccgtgg cggaaaccga
     2161 acctcagctc gtcatcaacg ccgccggcta caccaatgtg gacgcctgcg aaaccgccaa
     2221 ggaagaatgt ttcgcggtaa acgcggaagc ggtcaaaaac atcgcccagg cctgccggga
     2281 cggcaatgtt cccatgattc attacagcac ggactacgtt tttgacggaa acgcgaatca
     2341 cccttacgcg gaagaagatc cctgcaatcc gatcaacact tacggcgcct ccaaactggc
     2401 gggcgagcac catctgaagc gtctgacgga caattttatt ctgatccgga ccgcctggct
     2461 ttacggcgca aaggggaaaa acttcgtttc ggccattctg gaccgggcca gggagacggg
     2521 aaaactgacg gttgttgacg atcagatggg ctcgcctacc tgcacgcgcg acctggcggc
     2581 ggcgacggag cttctggtgg acaaaaacgc ccgcggcatt ttccacgtaa ccaaccgcgg
     2641 ctgctgcacc tggtacgatt ttgcgcgcaa aatcctgaag gaagcccgca tggagcaggt
     2701 cgaattgtcc ccgatgaaaa ccagcgaact gcagcgggcg gcccgacgac cggcttacag
     2761 cgtgctcggc atgcacaagt tcgtggccgc aaccggcaaa accatgcagc cctggcagct
     2821 cgccttttcg gattattatt cgttcatcgc cagccggacc tgcctgagcc cgccgggaac
     2881 cgcttgatgc atccggcaca aacgcttttt agcgcggcaa atttgcgaaa gaattttaaa
     2941 atgaattccg gccggacgta tgttataatt ccaaaaaatc ccgtcaggat ggaggtttga
     3001 ccgtgcgaaa aatattcaaa gtagtctttt ggggtctgat tccggccgcg gctctgatgc
     3061 tgcagggctg cctcgtctcc acgccatcca cgctcattac gccggaaatc cggtctttgt
     3121 atgaagggga atataccgtc gatccctaca tgaaaaaaca tgttcccctt tccgttgccg
     3181 ttctgccctt tgtcgatctg tccggcagca aggaaggggc aaacgccatg cgcaaaggct
     3241 tctacaacca tttcagctcc cttcccttca aagacatgga actttacaag gtggacgatc
     3301 tgcttttgaa ggcgggcttg accgacccgg atgtcatccg caaaatccct ccgcgaaagc
     3361 tgggagaaat tctgggcgtt gacgcggttg ttttcggaga ggtttccgat tttgacaaat
     3421 atttcgcggt cctctattcg caggttgccg tgggcgcgga agtgaaaatg tacgacgcca
     3481 aaaccggcaa ctttctgtgg agcggaaaac ataaagtgcg caagcacgaa ggcggcgttt
     3541 ccacgaatcc ggtcggcatc atcgcgacgg taattgcgac cgccctgaat gtccgcgaca
     3601 ttcagctcct gcgcgccaac gatgatctgt tccgcgacat ggtcaaaacc attcccacgc
     3661 cgtctctggc tcaggccaag cgtccgccgg tcattacgct ccttacgcag gacaccaaag
     3721 gactgccgaa aaaagcaggc gatgagatca aggtggtcat tcagggaacg cctaacatgc
     3781 aggcttcttt tgacatcggc cagtaccgga agcgcatcga aatgcaggaa gtcgagccgg
     3841 gcggttatta cggcgtttac aaagtggtgc ccggggacaa catcgaaaaa gccatcgtca
     3901 ccggctttct cacggacgat tccggaaaca ccgcccactg ggtggacgcg atcggatccg
     3961 tcacgctgga caccaccgcg ccggacaaac ccgcgggcat tacatcaacg ggaagaaacc
     4021 agcgggtggc gctgcgctgg gagaaaaaca ccgcggcgga tctggccggt tatctcattt
     4081 accgctccga gaccccgctt tccggatacc ttcaaatcgg caaaacggaa ctgaacgagt
     4141 acaaggacac cgatcagaat ctggcaaacg cccggaccta ttattaccgg atttcggccg
     4201 tggattttgc aggcaatgaa agccggacgg agcaggccga gggcatgccg atcgcaccgg
     4261 ggccgacgcc ggtttccgga gcgatcgagg cggatgccac ctggtattcg ggcgccagcc
     4321 cctacattct ggaatccaat gtcaccgtga aggacaaggc gcttttgacc attgaaccgg
     4381 gaacggaaat tcagtccaaa ggcggcgggc tcgtgatcga aggacggatt tcagcccggg
     4441 gcgacaatga ccgcatcatc cgttttgatt cggcgcagga cggtcagttc tggccgggca
     4501 tcacctttgc cagcgtgaag gaccgggaaa accatctggc gttcgtccgc gtgcaaaacg
     4561 cgcggaccgc cgtcttttgt caggcctctt cgccgaccat tgagcaatcc gaattcacga
     4621 aaaaccacga agcgatcaaa atccagggag tcttttccaa gccgctggtc agccgaaaca
     4681 ccctttacaa aaacaagggc gccgccattt ccgtaagcga cggagccgcg ccgcgcattg
     4741 tgggaaacac catcaacgac aacgaccata cgggagtggt gctgcactcc gcggcggcgg
     4801 aaatcacgca aaacacgatt acccggaaca aaaacaacgg catcgcggtc aaaggcgccg
     4861 aagcggtcat ctttcaaaac aacctggtgg acaatcagcc ctacaatgtg ttcggcgaaa
     4921 tgaaaggaga gcccgcaaag gcgctgaaca actggtgggg cagcgccgcc atggcggccg
     4981 ttctgtccgg aatacacgga cgcgtcgatg tcagctccgt gctggacgcc ccgtacccgc
     5041 agggcaaagc gctggccgtt ccgattctgg atcagaagct ggaaggcgcg atccaggcgg
     5101 atgcctatct gattttatcg cgaagtccct atcgcctggt caaagacgtg gtgattgacg
     5161 gaggcgccac tctctacatc gaacccgggg ttgtcatcga atacgaacag gaaacctcct
     5221 tcatcaccag aaacggcggc atcgtcgcga agggaaccgg ggaaaagccg attaccttca
     5281 ccgccgcttc cgcatcaccc gctccgggct tctatgtcag cgccgtgcgc ttttccgaag
     5341 ccacaaaaat caacagcgcc tttgcctatt gcatcgtgaa gtacgcgaca accgcctttg
     5401 acatttacac gggatcgccg gaaatctccc acagttacat cgcccacgcc tcgcagaacg
     5461 gcgtttactg ccgcaaagac gccgccccgg tcctttccca caacacgttt gcgcacaatc
     5521 agggggaagg cgcggtcaaa tgcgtcggtt cggccaatcc caagattttt caaaacaact
     5581 tcatcgacaa cgccgtggcc attcagagtt tttcgtcgat tttcatcgac gcccgcaaca
     5641 actggtgggg ggcttcgcct ccggatccca aaatcttctg gggagaaaac atcaacacga
     5701 atccctggct ggaacaggca aacccggagg cttttacgga aagacgttga caccggaagg
     5761 ttattttcct atagtcccgc agacttgaaa acggagtgct gtgggtgatt tatttccgca
     5821 atgccagttt gtcttttctg gacaaaacgc tgttcgaccg gattcaatgg acgattcatc
     5881 ccaaaagccg catcggcctg gtgggcgaca acggcgcggg caaaacgacc ctgtttcggg
     5941 ccattctcgg cgaggtgcct ctcgattccg gtcttatcga aatccccggc cggaagcaaa
     6001 aaaagatcgg ctatcttccg caggacctgg tcgaactcga atcgacaggc ctgatcgagt
     6061 atcttcgccg gaaaagcggc attgccgggc tggaggaaga cctccgctct ctggagcggc
     6121 aaatggcgca ggcgccttcc cattcgcgcg aatacggcga actttcaagc cgttatgcgc
     6181 aggcaaccgc cgaattttcc gcccgcgagg gatatgcttt tgaagcacga gccaaacaga
     6241 ttctcaaagg gttcggcttc cggccccagg attttgaaaa aacctgcgac gtgttttccg
     6301 gcggctggaa aatgcggatt ctcctgacct caatcctgct gggccggccg gacatcatgc
     6361 tgctcgacga gccgaccaat catctcgaca cggagagcat ggaatggctg gaaagctacc
     6421 tgaaggacta ccccggaacc attctgattg tttcccacga tcattttttc ctggacaaga
     6481 tcgccacgca aatcgcggaa ttagccaatc gccgcatcca catttacaag ggcaactaca
     6541 cctattatct tgccgaaaaa gaccgccggt ttgaagcgct gaaaaaagaa atggaactgc
     6601 aaaaagcgca gattaaaaaa attgaatcgt ttgtggagcg gttccgctac aaggccacca
     6661 aggcgtcgca ggtgcaaagc cgcgtcaaga tgctggaaaa atttaaagac cttcaggcag
     6721 aaggcgccgc caaaagcgtc gtcatgaaat tcccggaagg caaaaaaagc gcaaaggaag
     6781 tggtcacggt ctgcgatctg ggccatgcct atggagacct ggcggttttt tccggtttga
     6841 atttttcgct ttttcggggt gacaaggtgg ccttcgtcgg cgtgaacgga tcgggcaaat
     6901 ccacgttgtc taggcttttg agccttttcg aaaggcctgt ccgcggatcg gtgacgtatg
     6961 gagaaggcgt caacatggct tttttctctc aggaaagcgc gcagaacctg aactacgcca
     7021 acaccatctg ggaagaagtg cagggagttc cgacgtccgc cgacgaccag aacaagcgaa
     7081 acctgctggg agcttttctt ttttcgggag acgatattta caagtccgtc ggcgtcctgt
     7141 ccggcggaga aaaatcccgc ctggcgcttt tgaaaatcat gctcaccgac accaacttcc
     7201 tggtgctgga cgaaccgacc aatcatctgg acatcaaaac caaagacgtt tttcaaagcg
     7261 ccctgatcca ctattccggc acaatggcca ttgtttccca cgaccgttac tttctggacc
     7321 gtctggtcaa ccgggtcttt gaactaaagg acggatccgt cacggattac gcgggcaatt
     7381 attcctactt tattgaaaag cgcgcccgga tggcggcgga tgccggacag caggagcgcc
     7441 gcgaccgggc gcctgtggac aggacgctta aaacaaaaga caaaaaaaga ctggaagcgg
     7501 aagaacgcaa tcgcctgtcg aaaatcaaaa acggcttaaa gagggagctg gcgaagttgg
     7561 aagaaaaaat cgcagagctt gaatcccgaa aggctggcga tgaacattcc ctgtgtgatc
     7621 cccaaacaca caaagacgca gtgaaaatca aaggccttca gatcgatctg aaggccgttg
     7681 aaaacgagct tgcacaagcc taccacctgt ggacggaact gagttgcaaa ctggaagaga
     7741 ctgattcaga cggcttcgcc tgattccaaa tcaaaatcaa gcgttccctt tttggcgtcg
     7801 ttgatttcct tcatggattt gcaggaaacg gttttaatcc attccctgcg cgcagtggtt
     7861 cttcttacgc gccgtccaaa cccgagcgtt ccatgtgctg ataccaggtg gccggatcgg
     7921 ccaggaattc ttccgtgtcc ttcagcgaca gcaggtgctg ccgctccacg gaggcaagca
     7981 tattgaaaaa cgcgatctct ttggcatccg tggactgctt ggccagcttg gtataaaggg
     8041 ccacgccgcg cgcttcaaaa tcaattgccg tgcggaccgc cttgagctca tcatccgttc
     8101 cggcaatgcg ggctgatttt ttcccggaca tgccctgcag gatggctccg gcggcagacg
     8161 gccggacttt cagggcgatc gtggccggcc atttcttctt ttcctcccac ttcgcgtgga
     8221 gttttttgag catttcgtaa tgctctttct cctcgtcggc aatctgcttg aacatgtttt
     8281 tcccggccat gttcctggtc ttccgggcat gggcaagata aaactcgcgc tccttttctt
     8341 cgttttccaa agcaaaggcc agtgcgttca ttcttttatc catgaatacc ccctttggcg
     8401 gatgatcgtg gttgcgcggt attatagaag atgctgcaaa ttcaggcaat acgttttttc
     8461 gggtcccggc tatttcagga agccctgaat gatccggttt acggcctgat cgtaggtcag
     8521 ccccaggctc atcagtttgg tcagctggtc attcgcgatc ttgccgatcg aagcttcatg
     8581 ggtcaattcc gcgtcgggat ggccggcctt cagggaaggc atggtttcgt tggtgccgtt
     8641 gtccatgatg atcgcgtcgc actcaatgtg accgtagcac tggttctgcg cgtcgatggt
     8701 cgcgtaaaac gtctgtttgg aattgccctt catcaccgaa cgcgaaatca tgttggcccg
     8761 gcttcccctg cccttcatca cgatatcatt ggtggacacg gcggtttgtt cgccgtcggt
     8821 cagaaccctc tccgtaatga gcaaaaggct gttcggacca aggaccgctt cattgacacg
     8881 gtgggtatca tccaccccgc cgatctgcgt caattccatt tccgcgcgcg ccccctcggc
     8941 cagaaacacc ttggtcgtcg gattcagcga tcggcgtccc gtgccttcgc cggacgcgta
     9001 gtgtttttcc acgtacgtca ccttcgcatt tttcccgacc ttgaattcgt gaattcccgc
     9061 gtgcccttcc ggttcggatg agccgcagtg aatcccgcag ccggcaataa tcgtcacttc
     9121 cgcgtcgtcg ccgatgacaa aggcattctg cacgacgtca tacaggccgg cctggcttaa
     9181 gatcacggga atatggaccg attcattttt cgttcctggt ttgattttga caataatgcc
     9241 ggtttcatct tcgttggttt caatattgat gttcgccgat acatgccggt ccagcagttt
     9301 gccgtttttt cgtatattgt aggcgccttt cggcaattcc tccagttcgg cgacggtttt
     9361 cagcaaggct ctatcgatag catccagcat cacgctctcc ttcacaactg ttccggtacg
     9421 tacacacgct caaatcgtcc agcagcggca tggcgccttc gaggtctccg tcataaacga
     9481 tcctgcctga attaatgatc atcaccacat ccgccgcctc caggaaggct ttgttgtggc
     9541 tgaccacgat ggtggtggtt cggttttcgg ccagttcctt tttcagaagg ctgaccatcg
     9601 gaccgatagt ccataagtcg atgcccgtgt ccggttcgtc gtaaatcgcc agtttgggat
     9661 ttgcggcaat ggtcgtcgcc agctcaattt ttttaatttc ccccccggac agcctggcat
     9721 ccacttcctt gtccagaaaa gacagggggc aaatgcccac ccggcccaga atctccagaa
     9781 gcctgggttc ctcttcgatg ccggtggcaa tcgtcagcag gtcgcgaaac gtaatgcctt
     9841 taaagcgggc gggctgctga aagctgtagg cgatgccttc tttggccctt tgggtaatcg
     9901 tcagggtgga gatattctgt ccgttgaaaa gaatcttgcc gccggataac gggttgacgc
     9961 ccatgattaa cttggccagc gtggtcttgc cgctgccgtt cgggccggtg atgacgtaaa
    10021 acttcccgtc ttcaaaaacg taatcaatcc cgtcaatgat gttgcgcgga ccgccgtcgc
    10081 ttccttcagt gtcctgtacg gtgaaataca ctttctgtag ttccagcatc gaatttgcct
    10141 tttctctcaa gatttgtgcg ttatggatat atgtcgcttt gcggattctg tcaatagaca
    10201 gctcatcctc cgtttcttcc ccatccacta tccaccgttt accgcctgtt ccgcttccgg
    10261 ccgccgcgct tttcctgacg ctctttggcc ttccggtgcg catacgccgg accttcccca
    10321 gcggcccggc acagatgcgc gcggcggtgc acatcgccga agccggtcac cgcccccttg
    10381 acctccggca ccggcgcctt gacgccaggc gaccggcagg gccggagcat catattttca
    10441 tcatgaatca cctctggaag cggcggatcg tcggaaaaga aagagatgga gcggatactt
    10501 aaagagcagg gaacttgcgt ccgcctgctc tttaaatgta gatgactgat caatattact
    10561 tcttggctgc ttttttcacg accttctttt tcgctacggg cttcttcgct gcaactttct
    10621 tttttgccgc cggtttcttt gctgcgacct tcttcacgac tttctttttc gctgccggtt
    10681 tcttcgctgc ggccttcttg acgactttct ttgctgttgc tgccattggg tttagcctcc
    10741 tttaattgag tttacttcat cttttcaaaa acccgtaatc ttacggatgt ggtgcaatat
    10801 tactttattt ttttctttcg gtcaacaaaa actataaaat atttttaatt tttttctttt
    10861 tttcaaaaaa gaaatttttt tgcaattgaa aagtccgtca aaagaaatcc ggtcgaagca
    10921 caacggaatg aatcaatgta atcctttggc agcaagactt tcagagtttt ccgatcagag
    10981 atacgttttt gctccgatga agcgccgggc atagtaatcg gaggcgagcg agtcgatgcg
    11041 gatttttttt ccgctggacg gcgcatgaat aaaccggccg gcgccgatgt aaatccccac
    11101 gtgagacact tttccacctc cgcgcgtgtc aaaaaaaacc agatcgcctt tctgcagttc
    11161 ctgccgggcg atcgaatctc ccgcgtcgaa ctgcgttcgc gaatggcgcg gcagatccag
    11221 gccgttgagc tgatagaccg tcatggcgag gccgctgcag tcaaagccgg tgtccgcgga
    11281 cacaccaccc cacagatacg gcacgccgat aaaatcgtga gctgttttga ccagcgactc
    11341 gcgcaaataa tccgtcccgt attgctttcg tttggccgcc ggggaatctt ccggcaaaac
    11401 gatgtagtat tcttcgatca cgcccgcctg cgcgagagac tccgcgcgga gccgcgccgg
    11461 ctctttcgtc gagaagtttc cgaatcgcac cttaaacagc ccgtcgtccg ccctgaaata
    11521 ggtggcaggg agtccctggc ctttgagcct ttgcgtcagg cgcacggcat tttccacccg
    11581 ggcaaacgcg cccgcctgaa tcgtgtaccc catctcggcg agctgacgct tctgcggccg
    11641 gtgcgaaccg ggatatactt ttgcttcgcg acccgcgcag gcctgaagga gcaagcccat
    11701 catgaaaagc accgccagcc gcaagaggat gggcttcatc ttcccttttg cacgcatcgc
    11761 cgcgctagaa tttccatcgt gtctgaacaa acgccaccgc ctcttctttt tcatgaatcc
    11821 tgttttccag ctgttcttct tccagcgccc gcaggatttc ccccatttgt tttcccgggg
    11881 caaaacccat cgccagcaga tcatcgccgg tcagcagacg gggcggatgc agatcttccg
    11941 gctccgtctt ttgcagctgc tccacacaga acgcataatg gtccagaatt ttgtggctgg
    12001 ccaggcaatc cagccggtgc aactgcagat gcaggtgaaa attcggcatc cgcagaaacc
    12061 gcttcaagcg ggcaggacgc attttctgca cgttcatgaa agccatgtgc tgacgaatca
    12121 ggtccagaac ggtttcccgc agagcgcgtg aaaaccgcag gcggcgcatc acgtcttccg
    12181 cgatggatac acccgtcacc gagtgcccgt aaaaatgaac gcctttttca tcttccgtcc
    12241 gtgagacggg cttgccgacg tcgtgcaaaa gcgctcccca ggccagcgcc gcctccgcct
    12301 ccggccggtt ttcgttcgac agaatctcca tcatgatcag cgtgtgccgc cagacatcgc
    12361 cctccggatg aaaacgcggc ggttgcgcca ccccccgcgt ccggtccact tccggcagga
    12421 tgtgcttcag cagacccgta tcggccatta attccagacc ccgccgcgct ccgctccgcg
    12481 tgagaatttt attcaattcc tcctgcacgc gctccgcgct gatctcccgg attttcgcgg
    12541 tgtgccgcac catcgcttcg tggacgccgt cctccagatg aaaatccaga tgggcggcaa
    12601 aacggatcgc ccgcagcatt cttaaataat cttcactgaa acgaacggcc gggtcgccga
    12661 tcgtgcggat gatttttttt ccgatgtcct cccggccgcc gacgtaatcg acaatgtcgt
    12721 tcgtttccgg atccatcagc aggccgttga tcgtaaaatc ccgcctgagg acgtcttccc
    12781 gggccgatga aaagcgaacc tgcgtggggc ggcgtccgtc ctcatatgct tcgtcgctgc
    12841 gaaacgtggc cacctcatag gaaaatccgc cctcgcaaac caccaccacg ccgaatttgg
    12901 cgcccacggc gacggtgcgt tcaaaaagac ggatcacctg atccggcggc gcggacgtgg
    12961 cgatgtcata gtcacccgga gaaaccccgc gaacaaaatc ccgaacgcag ccgcccacaa
    13021 aataggcttc aaaccccgcc cgccgcaggc cttcgacgac cagctttgcg cccagccgtt
    13081 tttcgatgga atgatcatcc gcctttcgga tcaatcttct ttcctcgtgg cgattggatt
    13141 tatttctttc ccagccggat cgcgcagaat tcgccgcaca tggtgcagcc ttcctcattc
    13201 gcgcttttgc tcttttcaag gagcgcggac gttttgtccg ggtcaaggga ggctttcacc
    13261 tgcccctgcc agtcaaattt cctgcggcac tcggacattt tccggtcccg ctcaaaagcg
    13321 cccgggatgc ctttggcaat gtcggcaata tgcgcggcaa tccgcgcagc catcacgccg
    13381 tcgcggacat cggccagcgt gggcagacgc aaatgctcgg ccggcgtcac gtagcacaga
    13441 aaatccgcgc ctgcggcgcc ggcaatcgcg ccgccgatgg cggaagtgat atggtcgtag
    13501 ccgggcgcaa tgtccgtcgg aagcggcccc agaacataaa acggagcgcc ctgacataat
    13561 ttcttctgaa gtttgatgtt ggcttccact tcggtaatcg gcacgtgacc gggcccttca
    13621 atcatcacct gcacaccggc ggcgcgggcg cgggcggcca attgccctaa aatgattaac
    13681 tccatcagct gcccctggtc ggtcgcgtca tggatggcgc ctggccgcag gccgtccccc
    13741 aggctcaaaa ccatatcgta gcggcgggca atgtccagaa gccggtcata ctcctcgtac
    13801 agcggatttt cccggtcgtt gcatcgcatc cagttggccg tcatcgaacc gccccggctg
    13861 acgatgccca atactcttcc ctgtgcttcc acggcagcca cgctttcacg attgacgccg
    13921 caatgcacgg tgatgaaatc aacgccatcg cgtccgttct cttcgatcac cccaaacaga
    13981 tcatccggcg tcatttctga aatcgctttt ttctcttcca gtgtttttac cgccgcctga
    14041 taaatcggca ccgtgccgat ggcgacgctg gacttggcca ttacggcctt gcgaatgtcc
    14101 gcaagattcc cgccggtcga aagatccatc acggcatccg cccccgccgc gatcgccacg
    14161 gacaacttct ccagctccag cttcaaatcc tgattgtccc tggacgttcc gatattggcg
    14221 ttgaccttgg tgcgcagccc cagaccgatc gccagcggct ctatggccgt gtggtttata
    14281 ttgcggcaga tcacgatggt gccatcgacg atgccctgtc ggatggcttc cggcgaaacg
    14341 ccttcccggg ccgcactctg tttcatttcc tcggtgagaa cgccctttcg ggctttttcc
    14401 aactgcgtca tgacttcttc tcctttttta ccccgaaatc cccccacaga ttcaccggac
    14461 cgtggcccct gcccagtgac agcgaatgct gaatggccct ttcggtcatc ctcttcgcct
    14521 gccggacggc ctcctgcgtc ccgcgcccct gcgcgagaca ggacgccagc accgaagcaa
    14581 aggtgcagcc ggttccgtgg gtgtttttcg tttgcgtcca ttttgccgca aacatatcat
    14641 accgaaggcc gtcataaaga atatccacgc agccccgggt ctttcttccg ggaaggtgcc
    14701 cccccttgac caccacccgc tttacgccta aatccgaaag gacggcggcg gcttttttca
    14761 tatcctccac ggaacggatt ttcatgcgcg acagaaattc ggcctccgga atattcggcg
    14821 tcagaacgtc cgccagcggc agcagcgttt tcacgagcgc ccgcctggcc ctgtcggtca
    14881 tcaaagagcg cccgcccttg gcgatcatca ccggatcgac cacgatattt ttcagccggt
    14941 attgtttgat ctttccggcc accgtctcca ccgcttccgg cgtcatgagc attccggttt
    15001 tgacagcgtc cgcgccgata tcctccagca cggaatccag ttgggccgct atccatttgg
    15061 gcggaacggg caaaacggac tgcacccccc gtgtattttg cacggtcacc gcggtaacgg
    15121 cgcacacggc gtaaccgccg caggcatgaa ccgctttcag gtccgcctga atcccggccc
    15181 cgccgccgga gtccgacccg ccgatgcata aaattcggat gggctttccc attcagtccg
    15241 cctccggaaa aaaatctttt tgccggtagc gaatgaccag cagatgaccc gagcgaaaac
    15301 gaatgcgggc gcgctctgcg gcaatgatgt tgctcacgcc gcgcgattcg gaaagttcaa
    15361 aggttccttc ctcgagcgga taagcaaatc cctccagcgt aacgccttcc acgcggggcg
    15421 aaagcgccag gagggaaacg aggcagcctt tcctgttttc aaagaccgca tcatcaacgg
    15481 gcacaaaaac ttcacagtat tcatccagca gacaggcggc aacacccgct tccttcgccc
    15541 ggaccagcaa aaacaaattg gccagagcat ggtcgattct tccgcccagc gccccccaga
    15601 tctgaatcct ttcgggcttc agacccagag cgtaatccag ggccagagcc gtatcggtaa
    15661 aatctttgtc ggccggatgc tgaatgattt tcacacccct gcgcacataa tccgccagac
    15721 gggaagattc aatagaatcc atatcgccga ccagcacatc cggtgtaacc ccggctgccg
    15781 ccagatgacg ggcgccgccg tcacagccaa tcaacaaatc gcttcctgcc tccgacattt
    15841 tcttccggaa aaagtccatg tcgccaagac gcccgccgct gataatgaca atgcaccgat
    15901 tcatggatta ccaaaaaaaa taagccatac cctgtcacct gaaaaggata tggctggaat
    15961 tgtctttaga atgattgcca ctccctacgc cggcattacc cgggtcaggt tcaaagggta
    16021 tattctcagc cgtttctttt ccggcctgaa aaaggagcgg caccccgtgg acgaaaagta
    16081 gtacattaac cgccaaaaag caacggatta aatggccgct tccggctcat cttctccttc
    16141 gtagatgcag ccggattcag ggctttcctg aagaacgatc ctggacaact gcggcaggac
    16201 gggtttcagg cgcagccaga tccagcggca gagattttcc gaagtgggat tttccagacc
    16261 ctcgacggcg ttcaaatttt tgtgatccag ttgctccagg ataggctcaa aagcggcggc
    16321 aatatcggca aaatccgtca cccagccgct gtctgaaccg gctttcccgc gaaggtgaat
    16381 ttcgacgcgg aaagaatgac catgcatctt cgcgcatttg tgtccctcgg gtacgcgggg
    16441 aagcgagtgc gccgcgtcga aatgaaaaac cttgaagatt tccatcatcg ttatcgaacc
    16501 cctaaaactt tgtgcatctg cagactgatt cgccaccggg gatgcgccag aacgtacgca
    16561 agcgaaagcc tcacggcttc tccaccgtgc ggagaatcca tcggctgcag gaaaaaatgt
    16621 tgaaacgcca ggcgttcgta ctttttcggc tcggcgccct cctgcgggaa aaccagcttc
    16681 aattcgtctc ccgcggcctg tttcagcggg gcgtcggctt tcgggctgac gcagatccag
    16741 tccaaaccgg cgggcgcggg aagagtgccg ttggtttcca cggctatttc aaagttttct
    16801 ttatgcaggg cggaaatcaa cgcggcatcc agctgcaaaa gcggctcccc gcccgtgcag
    16861 accacaaaag gcctggtgag cttatttttt ttgaccggcc aggccaggga gatcttgtgc
    16921 gccagttcgg cggaggatcg atagacgcct ccgttgatgc cgtccgtccc gacaaaatcg
    16981 gtgtcgcaga atttgcaaat cgacctggaa cggtcttctt ccctgccaga ccaaagattg
    17041 cagccggcaa aacggcaaaa aaccgccgga cggccggtgt ggtagccttc tccctgtatc
    17101 gagtaaaaga tttccttaat agaatacata agcgttttag gctgaagact gaagaccgaa
    17161 ggcaaaagcc ttcaaacctt cagccttcgg tcttcaatct gtatcttccc ggatctttaa
    17221 tgcctgcttc cttaaatcct ttcagcctga gcaggcagga atcgcaacgg ccgcaggacg
    17281 ctccgtccgc atccgggtcg tagcagctgt gtgtcagcga ataatcgacg ccgagctcca
    17341 tccccctttt gacaatttgc gctttgctcc aatgaatcag cggcgcgtga atccgtaatt
    17401 tctctgtacc ttccacgccg gcccgggtcg ccaggttggc caggcgttca taagcttcga
    17461 tatattcagg acggcagtcc gggtatccgc tgtagtccag agcgttgacg ccaatgaaaa
    17521 tattggagga cccgatcacc tccgcccagg ccagggcgta agacaggaaa atcgtgtttc
    17581 tggccggaac ataggtcatg ggaatgtttt tccccaattc ttccaccgag ccgtgtttgg
    17641 gaacggcgat gtccgccgtc agagccgacc cgccaataca ccgcaaatca atatcgaccg
    17701 tccggtgctc gaccacgccg ttttcccggg cgacgcgcgc ggccgcttcc agttcaacca
    17761 tgtgccgctg gccataacgg aaggtcaggg catgcacgtc aaatttcata ttccgcgcaa
    17821 tcgccagcgt cgtggcggaa tccacaccgc cggataggag caccaccgct ctggggcggg
    17881 tcttgatgga cggatcgttc atggaaaaac ctgtagcaga ttttgaagaa tttcgcccgc
    17941 gctatttcaa ggccggaggg ggaatgcaaa aaagcctgca ggcattccgt gccgcggtct
    18001 gcgccacgtc ctcccaggca agacctttga tttgggcgac tttcctcgcg gtatggacaa
    18061 ggaaggaagg ctcgtttctt ttgccgcgat ggggcacggg cgcgagatag gggcagtctg
    18121 tttccagaag aagccagtcc atcggcgcct gccggacaac ttcctgcagc actttggacc
    18181 ggtcaaaagt aacgacgccg ggcacggaaa gataaaatcc caaatcgagg cactgcctgg
    18241 ccatggacca gtcgccggaa aaacagtgaa agacacccga gcggatgccg gaggctttta
    18301 ccattcgcag cgtctgttcg tgcgcttcgc gatcgtgaat gatgaccggc aggcgcagct
    18361 ccccggccag atccaattgc cgggcaaaca tctccgcctg cttttcacgc ggggaaagat
    18421 cacggaaata atcaagtccg atttctccgt aagcgacaac cttcgggtcc tgcgcaagct
    18481 ttttcagctc gtcgagcgaa gagtcgtccg ccgcggccgc atcatgggga tggatgccga
    18541 cggtcgaata aatgttttca tgacggcggg caatcgacag cgctttgcgg ctcaaagccg
    18601 gattggtccc caccgtgacg atgcagtcaa cccccgccgc ccgggcgcgt tcaatcacgt
    18661 cggatcgatc gggatcgaac tccttcatct ccaggtgggc atgggaatcg atcagcatga
    18721 gggcttttcg ccgggttgaa tctgaatttc ttctgcgttt tccgaaacgt ccggaagctt
    18781 ttttaaaagg ctggacagat ctttttccga caattctccg atcagcagct tgcgcgcaat
    18841 gattctgcgg tctatcttca acaattccgg gtttactctt ttggacattg ggatcctcct
    18901 gaattcctcg acaaagttcc ccggctgcac cctctgcaac cgtggcgctc tagtattaca
    18961 ttttgccgac ataatcaagc cccggacgtt tggacgggcg catcggcttt tcatcttttc
    19021 aggcgcggat cggacgcgtc acgaatcccc tcgcccagca gattgtaccc gaccaccgtg
    19081 atgagaatgg ccaggccggg atacagcgac agccaccagg caatgtcgat gttgtccttg
    19141 ccggccgtca gaatatttcc ccagctcggc gttggcggct gcacgccgat gcccaggaaa
    19201 ctgagcgccg actccgtcag gatcgccccg gcaatgccca gcgtggccgc gaccagaatg
    19261 gacgccagcg cgttgggcag aatgtgcagg aaaataatcc gcgcatcatt ggcgccgatg
    19321 gccctggccg cccatacgaa atcccgctcg cgaagcgaaa taaaatccgc ccgcaccaga
    19381 cgggtgaccc ccatccagcc cgtcagtccg atcacgatca tgatgttcca gatggagggt
    19441 tccaggaagg caatcaccgc cagaatcaga aagaaagcgg gaaagcacag catgacgtcc
    19501 acaaagcgca tgatcaccgc atcgacccag ccgccgtaat agcccgcgac ggcccccagg
    19561 accattccga tgagaatggc cagccccgtg gcgacaaaac cgactttcag ggaaatgcgc
    19621 gcgccccaga tcattcggga aagaacatcg cggcccagct ggtccgtgcc gaaccaatgc
    19681 tctaccgacg gcggctccag aacgttttgc agatcgattg cgcccggatc gcagggcgaa
    19741 atcaggggag ccgccaggga aatcacaaaa agcagaacca caatcgccag gcccgccagc
    19801 gccagcttgt ttttataaaa catttcccgg aagcccatct taaaccctta agaaatccta
    19861 atgcggggat ccgccgccgc gtacgacaca tccgcgatca gattccccgc cagcgtcaaa
    19921 accgcgccga tcagcaaaat ccccatcacc gtcggataat cgcgcgccat aaccgacatg
    19981 taaaacaact gtcccatgcc cggaatcgcg aaaatcgttt caaagatgac gctgccgccg
    20041 atgagccccg gaatcgacag ccccagaatg gtgatcgccg gcaggagcgc gttgcgcagg
    20101 gcatgcttgt aaatcacctg gcgctcgctc aatcccttgg cgcgcgccgt cagaatgtag
    20161 tcctgccgaa tcacctccag catattggcc ctcatgtagc gggacaatcc ggccagccct
    20221 ccgaaagcag agataaaaac cggcaggatc agatgtttgg ccagatccgt ccactgcccc
    20281 caagtgctca gatattcata attgagggac cgcaatcctg aaatcggcag ccaacccagg
    20341 tgtatgccga aaaaaatcat catgaggagc gccagccaga aggtcggcac ggcaaaaccg
    20401 ataaaaacaa tcaccgacat caccttgtcg aaaagcgagt cctgatggac cgcagacaga
    20461 acgccgatcg gcacggccac cgcgatgatc agaaccagcg aaagaagatt gatcgtgatc
    20521 gtgatcggca gacgctccag aatcttgtcg gccaccggcc ggtggtcgga tgaaaaggat
    20581 gttcccaggt cgccgcgaat cagctttgtg agccaggata cgtattgaac gtggatgggc
    20641 ttatcgagtt cgtagaggct catcaggcgc tggcgcattt ccgccgatgc tttcgggttc
    20701 atctgcgtct gcagatccgt gggcgagccg ggagcgaggc gcatcacaaa aaaacagatg
    20761 atcgtgatgc ccaggagcag cgggatcata aaaagaattc tcttgaccag gtacgggaac
    20821 atcccccact cccccctcag aggttcaaac cgtccagata ggaactgttg cgcaaattcg
    20881 ccagagactg ccagaggtgc tcctcggcat gcgcctccag cgtaaccgtc gacctcacgt
    20941 ccagcttctt cagcgcggca aaaaacaggc cgaaaggaaa ggtggcgcct cccaccggca
    21001 gatgctcatc gaatttgccg aaattatcat gaagatgcag atggccgatg aagcggccca
    21061 gctgctccat ccagtcctcc aggggcgcgc gggcaaaaac attgaagtgg cccgtgtcga
    21121 aacaaaaaca cacctgacgg gaatggagcg cttccagcag gcggcgcagg atgcccggct
    21181 ccctttcgta aacgttttcc agcgcaatga cggctcctcc gtcttcggcc tgcacaatca
    21241 gctgccgcca gaaattgacg ctgttttcca gccaaagatc atcgcaggac acatagtagc
    21301 gttgatcaaa ggagggatga cagacaattt tcagcggctt aaaaatcggg atcagatcga
    21361 acacctgccg aatcctgtcc aggctggccc ggcggattcc gtcgtccagc gcgccgggcc
    21421 gcaaatccat aaaaggggca tgaaacgtca cttttaaccc ggcattctcc agcgttcggg
    21481 acaccttggc gcactgattc cgatccagcg actgaagcgc atcgtggctg aagtagatct
    21541 ccagattgag cttctttgcc agaaccatgg gcagatgccg ccccagcagc agatacggca
    21601 tgtgaacatg aactttccga atcatgtcgg gcattcagag attcctttct tcatcgacag
    21661 ggaccgttga tttaccacag tcgttttttt ttcacaacct cccatccctg ctcttccttc
    21721 tgcgacattg atgaaggagg caaaagctcc ctgcattcag ggaaacggcc gtgatcaccc
    21781 ggcagggacc aaaatgcctt attgtcaact ttttgcattt gtgacataag aaaattaaga
    21841 ttgaaggctg aagactgaag gttgattttg cgcactcctg gcgttataag ttttctgaat
    21901 gacggcgcgt ttagcgtctt tcattttatc cttaaagccg cgcgcgcccg caggggtgtc
    21961 ggcatcgtgc caccttgcag ggggtcagcc taaaaaccta ttcaaggagg aattttgcca
    22021 gttaaaaaaa atgaatcggc caggcggctg ctgttgtgca tcaacgcggt tctgcagaaa
    22081 aaggcaaaaa atattgttgt tttgaatgtt cgggagattt cttcctttac ggactacatg
    22141 cttatctgct cgggaacaac cgaccggcag gttcaggccg ttgcctccac ggttcaggaa
    22201 tatctgaaaa aagagggcat ccggccgctg ggtgtggaag gaaaggccaa cgccgaatgg
    22261 atcctgctgg attatgacga tgtggtgatc tccatttttc aggaagccgc ccgttctttt
    22321 tacgatcttg aaaacctgtg ggacgcgccc cgaatggaga tagacgaaaa cgcaacggaa
    22381 atcaagaaat tgcacaagga catgacttga ttctttatga gtttttatcc cagatctccg
    22441 atgctttttt ccccagaatc tgccttgcct gctcccgcat ccttccctcc ggccgcccgg
    22501 agttcttctg ttccgactgc tttggtcaaa tcacctttct tcacggaagc ctctgcccgg
    22561 tctgcggaat cctgtttccc gactcatctt ccgaagacca cctgtgccct tcctgcatgg
    22621 aaaacgcaca ctggttcgag ttcgcccgcg ccgccgttgc ctatgaggga atcatcgcag
    22681 atgtgatccg ccgcttcaag tacggacggg acctgacggc cggcgaggcg ctggcgggcc
    22741 ttctggccga ctttgatttt gacggcgtcg atttcagcgc ttttgacgcg atcattcccg
    22801 tcccgctgca catcaggcgg ctgcgggaaa gacgattcaa ccagtcgctt gtcctggcgc
    22861 gcgctctggg caaaaaacac ggaatcgccg tcgatttttc cttgctaaaa cggcgcagat
    22921 ttactttaac gcaaacagga ctggacaaaa gcgagcggga taaaaacatt tccggcgctt
    22981 tcagggcaag tcattcagaa aagattcccg gcgggagttt cctgctggtg gatgatgttt
    23041 acacgacggg agccacgatc aatgaatgcg ccaaaacgct ggcggaggcg ggcgcgaaac
    23101 gagtggcggc gctgacggcg gcccgtgtcc tttaaacgcc ggccggcctc acggcagccc
    23161 gaagggacag ccgatcacca acggaaccat cgggatcgtc ttcggaaaat gcgtcaaaac
    23221 gtttgcttgg ccgggacggc caaacggttt caataaaaaa gcccgggttg ccgacgaatc
    23281 aaaaggagtc gctatgaaca aaattctaaa cagaaccgaa ttaaaggaaa aactggatgc
    23341 gctgcgccaa gaggggaaaa agatcgcctt caccaacggt tgctttgatc ttcttcacgt
    23401 gggccatgtc cgatacctga aggaagccgg aaaaacggct gatgttctgg ttctggcgct
    23461 caacagcgat tcctccgtgc gggccatcaa gggactcaaa agaccgctgg ttcccgagca
    23521 ggagagggcc gaaatccttg ccgagctgga atgcatcgat tttataacaa tattttccga
    23581 gccgacaccg ctggagctga tttgcctgct caagcccgat gttttgatca aaggcgggga
    23641 ctgggcggaa gagcaggtcg tcggacgcga tgaaatccgg cggtggggcg gacgggtcga
    23701 gattattgcg gaagttgccg gcaaatccac gaccaatatc gtggacaagg tcatggcggt
    23761 gtacggaccg gacaaatgaa ggcggaacag gcgggtggat atgcgggatt atggattttt
    23821 ttcggatttt tatacatgat ttttttgctt tacaaaccag tcgccattag gtaaaaggtc
    23881 ccttcaaaca ttacggtaaa ggagtggcgg cattggaaaa acaaacgact ttaaagccgg
    23941 aaaagctcga agcatttcgc aaactgctga cacaaaaaat caatgaattg ctcagcgagg
    24001 cgggcaaaac cgtttcggaa atgaccagcg gcaaggaaaa tttccccgat cccaacgacc
    24061 gcgcctccct ggaatccgac cgcaattttg agctgcgcat ccgcgaccgc gagcgcaaac
    24121 ttatcgtcaa aatgcaggaa gccatcaaaa ggattgacga cggcgtttac ggcatctgtg
    24181 aatcctgcgg cggcccgata tccgagaaaa gactgctggc cagacccgtc acgaccgaat
    24241 gcatcgactg caaaaccaag caggaaaaaa tggaaaagct caaaggcgaa taaagaccgg
    24301 aagccccgcg agtcggggct tttttttggc ccgtttcccg tccgatattt tctttttgcg
    24361 tcaaaagtgg taacaagact catgttcata agcgtcgccg tcaacattcc ttccgataag
    24421 ctctttacct atgaagttcc ggtccatctc cagaacgccg ttgaggtcgg caagagggtt
    24481 tttgtgccct tcggcatcag aaagcgcagc ggatttattg tcgaaatact gaagaactgc
    24541 gatttgaagg acaccaaaaa gattgtcgcc gtccttgacg aagaggcgct gttcgacgcg
    24601 gaggatctga aattttaccg gtggatttcc gggtattttc tctatccgct ggggaaaacg
    24661 ctggccgagc tgattccctc gggaccggaa agaagggaac tgcgctggat cattcccgca
    24721 cccgccgccc gcgatgcgag gccgactgcc gcgcaaaaaa agcttctgga catcctccat
    24781 gagcattcag gcggcctggc ctttcaggat tggatccgga aatccaatat gaaaaacgcg
    24841 gcttccatcg cccgcagcct ccaggtggcc ggttgggttc acgtcatgga aaaggaaaaa
    24901 aagcaactgg gcatccgcat gaaaaaaatt atccggcttg ctgatgagct ctccggcccg
    24961 atgaaactga cgccccggca gcagattgtc gccgactgcc ttcggcggga agggccgatg
    25021 gagcttcgcg ccctggcgga gaaaaccgcg gcaagtgccg ccgtcatcaa aaggcttttt
    25081 gaaaaaggcg ccgtagaggt ccgcgaagaa gaacacatcc gcagggcgcc gatcgaatcc
    25141 gctctcgccg cccccagaca gaatatcgcg ctcaacgacg aacaaaaaaa ggccctggaa
    25201 accattgggc aattcatcga cagcgaggta ttccaccccg tcctgctgca cggcgtgacc
    25261 ggcagcggca aaacggaagt gtacctgtgc gccatcgacc gggttctgaa atcgggcggc
    25321 tcggccattt acctggtccc ggaaatttcc ctcacaccgc agctgatctc ccggatcacc
    25381 gggcgcttcg acccggacag gatcgccgtc ctgcacagcg gcatcgccga gagcgtccgc
    25441 tacgaccagt ggcgccagat ccggcggggc ctgatccatc tggtcatcgg cgcgcgctcg
    25501 gcactgtttg ccccgctgcc gaacctgaaa atcatcatcg tggatgagga gcacgatccg
    25561 tcgtacaagc aggatgaacg gctctgctac aacgcccgcg acctggccgt cgtcaaagcc
    25621 cggagcgcta acgccgtctg cgtgctcggc tcggcgacgc cggccgtgcg caccttcttc
    25681 aacgcccgcg gcggaaaata cgctcacctg gagttggccc gaagggtcaa ccgtcagcca
    25741 ctgccgcaaa tcgacattgt cgacatgaaa acgcaaaggg aaaccggcgg caaatcaccc
    25801 gttctttccg aagcgctcat cgcggcgctt cggaacgttc tcgatcgccg ggagcaggcg
    25861 cttctttttc tcaacaagcg cggcttcgac acgtttctgg tctgcgccga ctgcggctac
    25921 acgttttcct gccccaactg cgccgtttcc ctgaaaagcc atccggcgga aaatctggtg
    25981 aaatgccact actgcgatta cagccgccgc gccgtgccgc tttgcccccg gtgcggcgga
    26041 agccgaatcc tcaactacgg agcaggcacg cagaaactgg aggctgaact gcagagcctt
    26101 ttcccgctgg cgcgcatcag ccggatggat tccgacacta cggcgcgccg cggatcgcag
    26161 gaaaagattc tggcttcgct ggaacgaggc gaaatcgaca ttctggtggg aacgcaaatg
    26221 gtctccaaag ggcacgattt tccgtccatc acgctggtgg gcgtcgtttc cgccgacacg
    26281 tcgctcaata tgccggattt ccgcgccgcg gaaaaaacgt ttcagatgct gacgcaggtg
    26341 gcgggacgcg gcggaagagg cgaagcaacg gggcgcgtca tcatccagac cttcaacccc
    26401 ctgcattacg cgctgcgcca cgcgagcgcc cacgactact tatcctttta tgaagaggaa
    26461 atcgaattcc gcaaggcgct gaaatacccc ccgttcgcac aaatcatcaa cctgcggctg
    26521 tcctcggcca gaaaagcggc gctcgacgaa acggcgcggg agctgggacg agacgcccgg
    26581 gcgctttgcg ccgggcatga aaacattctg gaaatcatcg gcccggccga atcgccgctg
    26641 gcgaaaatcc ggggagaaca ccgccagcag atgatgatca aaggaaggga caaccggcgg
    26701 atgcattcca tggccgcaaa gcttttaaag aagcacgcca ccagcactgt caaaatcacg
    26761 gcggacgtgg atccggaaaa tttcatgtaa ggaatgccgg caaaaacggc agcggaaaat
    26821 ccgctgccgc cggttgggtc tgtttgaatc cagccgcccc caggaacgct aaaactgctg
    26881 cttggtctcc agccggatgc ggtcctgata gtactgcgtg atttcataca cagcataatc
    26941 gatgagcagc atgatccgca tgacggagtc gatggcctga ttgtgctcga tggcgttgat
    27001 gaaaatcgcc tggaactccg cgtagagcca catgtggcgg cgcatcatgt tcagggcgta
    27061 gatggattca tgaagcggaa tgcccatttc gtagcaggtt ctcgcgtagt gcatgaaaaa
    27121 ttcctgcgcc ttgtccagac gattgggggc catgagcatg ttccgaagct ggctgtaaaa
    27181 tttaacggcc tgcaggacga gcttctcttc aggaatgtcc ttgtagtgaa cggttcgttt
    27241 atttttcctg acgtcggcgg cccagtgcac cgcaatttcg tcaatattgg cttccagcac
    27301 atccagcagc ttaatcgatt ctaccatatc gcattctcct ctcttccatc ctatgcgtgc
    27361 aggggatacg gaaattattt cttgcctttt cccttcttca tcaattcctg atattccttc
    27421 gtgacttcat aaatcgcgta gtcaaaaagc aggatcgtcc ggctcagggt gtccagggcc
    27481 tgccgctgat cgatgccgga gctgaagatg gtctggaatt ccgcgtacag ccaggtatgc
    27541 cttcgcagaa gaatcaacgc gtagacggct tcatccatgg gaatccgcat ggcgaaactt
    27601 tcctgtgcgt aggttcgaaa atatttcatg gtggcttcac tgattttgtc ttcggcgaac
    27661 attttgctga aattgtgata aaactttaca ccctgattca cgatgctttc ttcgtccagc
    27721 tccttgtatc tttttgtccg ggcattgttc tggacgtcca tcgcccaccg ttttgccatt
    27781 cttcccgcat gctgctcggc cagctccaca tacttaatgg cgtatgtctt catgcttcgt
    27841 ctcctttcgt gaaagatcca cctgtcatga gattcgtttt gttgtcttca atataaagca
    27901 tgaaaccgaa attgacaaca ggaaaatgcc ggaaaaccgc cggtcaggag cggcccttga
    27961 cacaaacttc acaattccat atagaagcgt gaaaaagaaa aggacgggaa atgacgaaga
    28021 aaccacgcat tctcttcatg ggcactccgg cttttgccct gccggccctg gaaacgctct
    28081 gccaacgagg ctttccaatc tccgccgtcg tcacccagcc ggaccgcccc gcgggaaggg
    28141 gcaaaaagga tgccgctccc cccgtgaagc aaatggcgca gaaattcggc ctcacggttt
    28201 tgcagccggc aagcgtaaaa gacccgtctt ttctggaggc cctggacgcc gtccgtcccg
    28261 acatggcggt cgtggcggcc ttcggacaga ttctgcccaa agccgtcctt gacttcccgc
    28321 cgcagggctg cctgaacatt cacccttccc ttctgcccgc ctaccggggc gcggcgccta
    28381 tccactggag catcatccgg ggcgaaaaac aaaccggcgt taccatcatg ctgatggatg
    28441 aaggaatgga ttccggcgat attctggcgc aggagaaaac gcccgtcggc gaaacggaga
    28501 cttacggtga actgcatgac cggctcgcgc tgctgggcgc ggcgctgctg gtcgaaacga
    28561 tcgaaaggct cgcatccggc gccgttcaaa gacaaaagca ggattcttcc cgcgtgacct
    28621 tcgcgccccg cctgagccgg gaaaccggca gaatctgctg gcgcaatccg gccgaacaca
    28681 tcatcaatct ggttcgcggt ctgaatccgt cgcccgcggc ttacacgctc ctggaggggc
    28741 aggacctgaa aattcttgct gccgaggccc gcccgggccc tgtggaagaa ccgcccggcg
    28801 ccgtcgccgc agccaccccc gaggggctgc ccgtggcggc ctcgaacggc catgtgatct
    28861 tgaaggaggt ccagctggcc ggcaaaaaaa gaatgccggt ttccgacttt ctgcgcggct
    28921 ttccactgaa gcccggaatc cggctggaat gaggcccgac gcgcgccgtc tatttcccgg
    28981 cctggttttg aagttgtatc agatggtcgg ttgtttccct caggcgccgg gccatggatt
    29041 tcaggatcat ctgaaaatca ctggagattc cattaaactc ccggtcgaag tattcccggt
    29101 cgatcattcc gattcgggtt ttgcccaccg ccgttgccgt cgcggagcgc ccttctttgg
    29161 aaatgtaggc cacttccccg atgatttccc ccgttttcag aaccgcgatg acgatctttt
    29221 tcccttcgac gactcgggaa atttccacgg ccccctcttc aataacgtac atccagtcgc
    29281 caaaggttcc ttctttaaag atcacctgcc ggtcgttgaa ggtctcgtat tttacaatct
    29341 ggaacatggg gcgccccctt tcgccttttg gtgtgccgga ttcattgacc ggattttttc
    29401 taacatgaat ttcccatgaa ctcaaccacc aatcccaatg ggccggcccc cggaaattcg
    29461 cactttccat ccagccgagg ccctcaccgg aaaggggatc aagttcttct tgacttacaa
    29521 aacgggactt tctatagttc cgaggcaaag aaaagctctc gtgtgatctc atcgtgtttc
    29581 ggcaaggcgg caaaggccga ttccgggcgc gaaacgaacg gccgaaggca acgccggccg
    29641 gtccagcggg gtctggagac gggaaggcaa cggaggaacg gccatgcctg cagggcggga
    29701 agaccatctt gaaaaattca aaaagcgcta cccggaaaaa ttcgtccctg aaaaagacct
    29761 tttcaaatgc atccgccgcg gcaattccat cttcatcggc acggcgtgcg gcgaaccgca
    29821 gaacctgctc ggctcgctca tccgctacgt agaaaacacg ccccacacca tttacggaac
    29881 gcagatcatt cacatctgga cccttggcgt ggcgccctac gccaacaaaa agttcaaaat
    29941 caactttcgc cataattcgt tttttatcag cgacagttcc cgacaaatgg tcaatgaagg
    30001 gctggccgac tacacgccga ccttcctttc ggaaatcccc tccctctttc gccgcggaat
    30061 catcaccatc gacgtcgcac tggtgcagct ctcagagccg gacgaagaag gcctgatgag
    30121 cctgggcgtg agcgtggaca tcgtcaaatc cgccgtggag caggcccggc tggtgatcgc
    30181 gcaggtcaac cggcacatgc cgcgcgttcg cggcgatgcc atggtggata tccgcgacgt
    30241 ggatttcatc ctcccccacg acgagcccat cctggaatac acgcccgagc cacccggcga
    30301 agtgaccgaa aagatcggaa cctatgtctc gcgcatcgtg caggacggcg acaccattca
    30361 ggtgggatac ggaaccatgc ccaacgccat tctggcccat ctttcgggaa agcgggattt
    30421 gggcgtgcac acggagcttc tcaccgacgg catcatcgat ctcatgaaaa aaggcgtcgt
    30481 gaccaacgcg aagaaatccc tggaccgcaa aaaaaccgtc gcgtcgttct gcatgggaaa
    30541 ccgcgacact tacaaatacc tgaatgacaa tccggcgttt gagttcaggc cgatcgacta
    30601 taccaacagc ctctttgtca tcggacggca aaccaacatg accgccatca actccgcgct
    30661 ggaaatcgat ctcacgggac agtctaccgc ggaatcgatc ggcggaatct tttacagcgg
    30721 cgtgggagga caggcggatt tcatgcgggg ctcagcgctt gcgccgggcg gcaagtccat
    30781 tctggccatg cgttccacct cgatagacat ggctcattcc cgcatcgtcc cctgcctttc
    30841 cgccggcgcg tcggccacgc tcatccgcgg ggacgtgcac tatgtggtca ccgaatacgg
    30901 aacggcctac ctgcatggaa aaaacatccg ggaacgcgcc atggctctca tttccatcgc
    30961 gcacccgagg ttccgcccct ggcttgtgga gcaggcgcgc gaacggggcc tcatctaccg
    31021 cgaccaggcg tttatccccg gcacgacggg cgtttatccc gagcatctgg aaacctaccg
    31081 ctccacgcgg accggcctgc cgcttttcct ccgccccgtc aaaatcagcg acgagggaat
    31141 catcaaggcc tttctctact cgctttcgga caagagcatc tacacacgtt ttttcaccaa
    31201 catggtccac atgccgcaca acatccttca aaaatacgtg atcatcgatt actcgaaaga
    31261 aatgctgatg ctcgccatcc aggagcggca gggcatggag gagatcgtgg gtatgggcca
    31321 gtacatcatc gacacggaca cgcacacggc cgccgcctcc tttctggtgg gcgacgtctg
    31381 gcagaaccgc ggcatcggaa tcgtgctcct cgaatatctc accgaaatcg cgaagcggga
    31441 cgggcttctg ggcttttccg ccacagtgct ggcggaaaac aaagccatgc tccgtctttt
    31501 cgagcgaatg aattttcaga tggaaaagca gttttcgtcg ggcgtatatg agttgaccat
    31561 gggcttttac ccgagagaac cctgaacgtt tcgtttcccg ttttttcaca aagacacaag
    31621 accctgagcg gcaagacagt gcggtccaaa actttttgat ccgtcaaccg gcccaaccag
    31681 cattatcgga aggaggtcct gatgaaaaaa gtactgttca tgatggtttt gcttctggtc
    31741 cttttcgcgg ggtatttcct tttctggccg gtgccgatcg agccggtcgc gtggaatgcg
    31801 ccggctgcgg ccggatatac cggaccgcat gcgttcaaca ccggattgtc ccggctgaac
    31861 ctcatcgacc tgggcgccga agaggggccc gaacacatcg tcctgggaaa agacggaaag
    31921 ctgtatgtct ccgtgggcag cggaaacgtt ctgcgcatga atcctgacgg aagcggacag
    31981 gaggttttca tccgcaccgg cgggcgcgta ctgggatttg atttcgacaa agcggggaac
    32041 ctgattgccg cggatgcaat ccggggactg ctgtccgtca gccctcaagg gaagatcacc
    32101 ctgctgaccc ggtcggtcaa cggcgacccg atccgctatg ccgacgcggt ggtggttgcc
    32161 cggtccggca tgatttactt aagcgatgct tcgaggcgct tttcacccgc cgaatggggc
    32221 ggaccgtttg aagccagtat tctggacatc atggaaatgt cctccaccgg acgcgtcctc
    32281 gcctacgatc cggcgcaaaa atccacccgc gtcgtcgcca gtggattctg tttcgccaat
    32341 ggtctggccc tcagccggga cgagcaaaat ctttttgtga acgaaacggg aaaataccgg
    32401 ctttggaaag tgtccgtaca cgcgctggaa ctggacattg cccggccggg cgatcaggct
    32461 aaaattgttt tcgacaatct tcccggcttt cccgacaacc tgatgcgcgg acaggatgga
    32521 aaaatctgga tgggctttgc caaaccgcgc aatcccgatc tggaccaaat gggaagcaag
    32581 ccctttctcc gaaagattac cctgcgtctg ccgcgttccc tgtggccggt tcccaaagct
    32641 tacgggcatg tgttcgcctt cacggaagac ggccgggtcg tggccgacct gcaggatccc
    32701 ggcggcgcgt atccggaaac caccggcgtg acggaaaccg ccgaccgcct ttatattcag
    32761 agcctgcatg ccaaagggct cggctggctg cccaaataaa ggagtttccg aaagcaaacc
    32821 cgccgaagcc gggcgtgcta tggcgtgttt tttccttttt cggccagctc ttccaagagc
    32881 gcccggttca cgagccggat catggatccc ttttcctcga tcagcttttt gcttttcagg
    32941 ctgcccagag cgcgggaaag agattccggt ccggtgccga gcaggccggc caattgcagt
    33001 ttggaaatat tgagcgcgat ccgatcccgg tttccctgct cccgcgcgag atccagaagg
    33061 taggaagcca gacggccggg aatttccttg agcgacagat tttccacctg gacggtaaac
    33121 tcccgcaggc gggcggacag gagggccagg atattcatcg taagagacgg ctgacgggta
    33181 atcaggcgga tgaatttctc ccggttgaaa aacagaagat ggcagggggt caccgcgcgg
    33241 gcgttggcgg gaaaggattc cccggaaaaa acgggaaccg cgccgatggt gtctccctcc
    33301 ccgacgatat gaaggatctg ctccttccct tcggaagaaa gtttataaac attgacgatc
    33361 cccgcgacca ccagataaaa gccgattcct tcatctccgt catgaaagac catctccccc
    33421 ttgccgcagc gcctgggaac cgctatgttt tcaatctccg caagatgctc atccggcagt
    33481 cccccgaaaa gctggctctg ggtcaaaacg ttccgaactt tgttcatcat tggcgtcctc
    33541 tcggcaaaaa atgttttttt acttaacata aatcaaggat gtccggaatg attttcagta
    33601 ttctttccgg caaatcgcaa cgaatcgtaa caggaggtgt cacatgtttt gttatcagtg
    33661 ccaggaaacc gtcaaaaatg aaggatgtac ggtcaaaggg gtctgcggta aaaccgagga
    33721 aacatccaat ctgcaggacc ttctgattta tcttctgaac ggcatggccg tctgtgccga
    33781 caaggctaaa gaaaaaggtc cgctggatcc caaatacggg cgctttatga tggaggcgct
    33841 ttttgccacg atcaccaatg ccaacttcga cagcgaccgg atcatcggaa tgattcggga
    33901 ggcgcttcgg cttcgcagcg aactcagaca agcctgcggc gcggcgtttc ccgcgggcct
    33961 gcatgactgt gccgtgtggg attccgacaa catggaggaa tttaaaaaga aagccgccgc
    34021 cgtcggtgtt ctttccacgg aaaacgagga tgtgcgctcg ctccgaatgc tgctcctctt
    34081 cggaatcaag ggaatcgcgg cttatgcgga acatgccgcc gttctgggtt ttgaagacgg
    34141 gaaaattcat gatttcgtca gggaagcgct gatctccatc accgaagatt tgagcgtgga
    34201 tgaaatggtg gccatggtcc tgcgcgcggg cgaatgcgcc gtaagcgcga tggcactctt
    34261 ggacaaggcc aacacctcga catacggcca tcccgaaatt tccaaagtca acatcggcgt
    34321 gcggggcaac ccgggcattc tgatttcggg ccatgacctc aaggacctcg acgagctgct
    34381 gaaacagacg gagggaaccg gcgtggatgt ttatacacac ggcgagatgc tgccggccaa
    34441 cagctaccca gcgttcaaga aatacgccca cttcgttgga aattacggaa acgcctggtg
    34501 gaagcagaat gaggagttcg aatccttcaa cggccccatc ctgatgacga ccaactgcat
    34561 cattccggtc agggattcct ataaagaccg aatcttcacg acgggcgtcg tgggctaccc
    34621 cggattgaaa cacatccccg acagatccaa aggcggaaca aaagattttt ctgaaatcat
    34681 cgagcttgcg aaaaaatgcg cgccgcccag ggagttggaa aaaggcgaac tggtggcggg
    34741 atttgcccat catcaggtgc tggccctggc ggatcaggtg gtcgcggcgg tgaaatcggg
    34801 cgccgtcaag cgcttcatcg tcatggccgg ctgcgacggg cggcacaagt cccgcggcta
    34861 cttcaccgat gtcgcccggg aactccccga agacacgatt atcctgaccg ccggctgcgc
    34921 caagtaccgc tacaacaagc ttgcgctggg cgatatcggc ggcattcccc gagtgctgga
    34981 tgccggacag tgcaatgact gctactcgct ggcgttaatc gcgttgaagc tcaaagaagt
    35041 tttcggactg aaagacatca acgaactgcc gatttccttt gacattgcct ggtacgagca
    35101 gaaagcggtg gcggtgcttc tggctcttct gtacctgggt gtcaagggaa tccgcctcgg
    35161 accgaccctg ccggccttcg cgtcgccgaa cgtcctcaag gtcctggtgg agaagttcaa
    35221 catcaagccg ataacggatt caaagacgga tgtcgaagcc atggttgagg gccgttagtt
    35281 cattttcccg ctttcagggg aaaggcgcat tcaagcgccc gctcctcctt tgcacacccc
    35341 tggcagcggg gatgttttct gaaaatcaag agccaaaagg gaaaaacatg aaaaaaatat
    35401 tgcgtaaaat cattgaaatc gacgaggcgc tgtgcgacgg gtgcggccag tgcgtgacgg
    35461 gctgcgacga gggcgccctg caaatcatcg acggcaaggc caaagtcgtt tcggaaacat
    35521 tctgcgacgg tctgggcgcc tgcatcggcc agtgccccac cggggcgctc cggatcgtcg
    35581 agcgcgaggc gtcgcccttt gacgaagagg ccgtccaaaa gcacctggcc ggacaaagcc
    35641 aaccgccgct tccctgcggc tgcccttccg ctcagattca aattctcggc aaaccgtccg
    35701 cctgtgaagc ggccaatcag ccgaagcatc tggaggaggg cgaagaagaa tcagccctca
    35761 cccactggcc catacagatc aagctggtgc cggcttccgc tcctttcctg aagggagccg
    35821 acctgctggt ccttgcggac tgcacggcgg tcgcttttcc ccttctgcac cggaatctgc
    35881 tgaaaggaag agtggtgatg atgggctgcc cgaaattcga cgacgtgcag gaatacgttc
    35941 aaaagtttgc cgatattttt caaacggcaa acatcaaaag cgtcacggtg gccgtcatgg
    36001 aagtcccctg ctgttcgggc atgcccgtca tcgtccgaaa aggcatggaa gcggcaaagc
    36061 gaatcatccc catgacggaa atcgtgctga gtttaagagg aaaaatcctg aaacaaagct
    36121 gacggcttat gatcggccgc gtcggatgag ccgtccccgg aaaaccgggg acggccgccc
    36181 ggccttaggc cagtcccttc ttattgatgc cgtccgcgac ctcgtccaaa aattgttttc
    36241 attttaaaaa gcattccatt ttcctccttt gtaaattcag caagtgagtc tcacggccgt
    36301 ttaaaaccgc tggcccacgt tgagataaat ggacgaatgg cctcgctggg cctgaccata
    36361 ggccaggtaa accggcccga gatacgtttt gattcccagg aaaatgcttc cggccagaat
    36421 cagggactcg aaattgatgt ccgaccgttt gttccaaaca ttgcccgcct ccgccgacag
    36481 gccgatgtac aggggcatct tcagcgcgcc catgcccgca gagccgattt cccggtaggc
    36541 caccagccgg gccaggccgg tatggcgtcc gtagatttca ttggtggaaa agccggacag
    36601 attcaggaag ccgcccagcg gaaacgtgtc ctgtacggcc acgctgtcgt cgtccagcat
    36661 ggttttgacg tcaagggagg gaatgaaggt gtacttcccc cacgtgaagg cggtcatcca
    36721 gttgaagccc agggcctgtt catcgatgtc cgaacccagc gctttcaaat cgtaattcca
    36781 catcagactg gcgtccgttc ccctgagcgg aaaaacatag ttgtccagtc tgctgtaggc
    36841 aacggaagcc tgaacgctgc cgcggttgta ggtttcttcc ggcacgctgg ggtcgccgat
    36901 ggcaatcttc acgttgccgt gctcccggcg aaggcccgcg cgcacctggc cccagttgcc
    36961 gaactgcctg cccgcatcca cgccgaattg agcggactgg acgcggtact gcgtcaggat
    37021 ttcgcccttg tcgttgtacg tattgatgtt gcgcatgaga taggcggcat tggcggctgc
    37081 aaaatagctg agtgaatagt ccagcggctg gtaaagttcg gtatagatgc gcgggttctc
    37141 gccgattctc aattcggtgc gccattctcc ggccagtcgg ttcagagccg tcttggtaaa
    37201 ctgcgcggtc agggcataac tgccggagcc tttgaagttg tcctccaggc ccaggccgaa
    37261 ccggaaataa ttcggacccc atgctttgtc gaacggttcg atgacaatcg ccgtggactt
    37321 gtcttttttc cgaaggtgga agtccacacg ttcaaaggtg tcaatgccgt acacccggtc
    37381 gatatttttt ttcaattcgg gcatgttcag gggctcaccc ggtttgatgt tcacttgcgc
    37441 agctatcaga ccatcgggaa aagacgactt gttgtccacc tccacaacgt cgatcaccgg
    37501 catcgcctgg tcttttttcc tctgcgcccg cagataggcc ggaaaatcct cttcccgcac
    37561 ggccagatga gcgagctcgg tcttcatctc cgcggccttc ttccggccgg cggcgaccgc
    37621 gtctgccgcc ttgaagaaat cactgctgcc gatcgtaccc aactccggac gaatcaggat
    37681 gtccccttcc cgcatggtgc ggatctgcgc ctgggtgttg cgctgaacca tgatggaggt
    37741 caactgcgag gtgattccaa cggaggtgtc caactgatcg cggcggctca attccgtgct
    37801 gatatccacc acaatcacga catccgcgcc catttgccgc gcgatatcga tcggcagatt
    37861 gttggcgacg ccgccgtcca ccagaagctt gccgtctatt tccaccgggg cgaacagggc
    37921 gggaatggac atgctggcgc gaatcgcctt caccagttcc cctttttcca gaatgacggc
    37981 gtcgccggtt tcgatatccg tggccacggc ccgaaacgga atattcagcc ggttgaaatc
    38041 gtcaatgcct tcggtgtgaa tcagcaggga tttcagaatc aggttcagat tctgcccctg
    38101 aagcagccct ctgggcatgg caaattcacc gtctttgaag cccaggtcga ggtcgatcag
    38161 aaagtcggcc gcatctctct tccggcggaa ggtcatttcg ctcgggtccg gcttatcctt
    38221 gaaggcttca ttccactgca tgctggtgat gaattttttc agctcgtcgg gcgacatgcc
    38281 cgacgcgtaa attccgccga caatcgcgcc catgctggtt cccgcgatgc agtcaatcgg
    38341 aattttcatt tcttccagga cctcgatcac gcccacgtgc gccagcccgc gcgcaccgcc
    38401 tccgctcagc accaggccga tccggggacg cccgtctgga acggccggcc cggcctgaag
    38461 gtcggacgcc gcaatcagaa aagcgatgac gcacaggctg aaaagccaaa tccggttttt
    38521 gctcttatgg ccggaaggat caatggtttc cggtttcata tccgttccct ctaatgtcca
    38581 ttgtttaaga atgatcacct gcgtcatccc gccgccgccg ctttgccgtt acacggtatt
    38641 ccgaaggcac tcttcctttc gcatcaaaaa aaattccttc ccgttccagc aggcgccttt
    38701 tcatgtcggc gccgccgcca aaacgccccg gcgtcccgtc ggcccgaatc acgcgatggc
    38761 agggaatcac aagcggaaaa ggattgttcg ccatcacgga acccgccgcc cgggccgcgc
    38821 gcggaaatcc gaccctggcc gccagaccgg aatacgtgca cactttcccg cgagggatgc
    38881 gatgagtttg ccttaacacc cgccccgaaa attccgtcaa cccggataaa ttcagaaagg
    38941 ataaatccac gcgccgttct tttccaaggc acaaatcaac aatcagacga tccaggccgc
    39001 cggatatttt gcgcggcttt ttctcgacag cgggaaaatc ccgaaagatt ttttggataa
    39061 tcttttccct gcccggaaga taaatacact caacttgtgt ttttcccccg gcatcttccc
    39121 acaccaatcc gatttcgtca ttcccggcct tgatgatttg gtaaaacatc agacacctcg
    39181 tatagatttt caggctgaag gctgaagacc gaaggtcaat tttgttgacc ttcagcattt
    39241 ttattttttc tcgcgccttt gctgtgaaac aaaaacgttc ggtccttttt ccacgtcttt
    39301 tccttcagtc ttcagtctcc ggtcttcatc ccatatgttc gatcaatatc ttcacaccaa
    39361 tggcaatcaa aaccagaccg cccagaatct ctactttcct gccgaaaatc cgcgccaatc
    39421 ccttgccgaa aaccatgccc accgccgtca tcacaaaaca aaccatcccg atgatcgccg
    39481 ccggaaacag gatcggggtc tttaaaagag aaaaactgaa gccgaccgcc agcgcgtcaa
    39541 tgctggtcgc tatcgaaagc agaaagagcg gccagccgag cgtctggtcg gccttttcct
    39601 catccttttc cttttcaaaa ccctcccgga tcattttccc gccgaccaaa accaaaagcg
    39661 caaaagcaat ccagtgatca aaagcctgaa tcctatcgac cacggttgaa ccggccagcc
    39721 atccggcaag cggcatgaag aactgaaaaa ggccgaaagc cagggaaagc cgcagcaccg
    39781 gcgcccagga tttttttgca ttggccgcgc cgatggcgat ggccacggaa aacgcgtcaa
    39841 gtccaaggcc aaccgcgaga agaaaaatcg taatcaagct catatcgcac cgaactctcc
    39901 tggacaggct ttaagctgct ccacccgaag acaatacttt gtgaatagtt tcggccaggg
    39961 ccctgccgat gcccggcaac gcctgcaact cctcgggtga acagcttcga atccgctcca
    40021 gcgacccgaa atgccgcagc aaaatactct tccgacggct gccaaggcgg ggaatgtcgt
    40081 ccagcgcgga gcggaagtca tttttttgtt ttaatttccg gtgatagctc acggcaaagc
    40141 ggtgcgcctc gtcccgcagc cgctgcaaaa gggacagagc ggcgggtgac gacgaaagat
    40201 aaattgcgtc cttgcgccgg ggcaaataga cgcgatcctc cgattttgcc ggctttccct
    40261 gaagccttct tttggccgcc agaaaggacc tttcctcctt ggccaggccg atgacgtcca
    40321 gtttgatctt caagtcgctc aaaacggcaa gagcgacatt gagctgtcct ttgccgccgt
    40381 caaccaccag caggtcaggc accgggtccc gaccctggaa acggcgcgtc aacacctccc
    40441 gcatcatccc gtaatcgtcc ggctcatccg gaccgcgaac gcgaaagcgc cggtaggctg
    40501 atttatccgg ctccccgtcc tgaaacacca ccatcgaccc cacggcgttt ttcccgccca
    40561 ggttggagat gtcgaaacat tcgatccggc gcggcagacc ggcaagcgaa agcctttccc
    40621 gcaggaattg caaaacggct gttttctgtt cgtccttttt ccggtcggac gcctgcagct
    40681 cgcgggcatt ggcgcacgcc atgtcaacca gcgccttttt cgcgccccgc tggggcacgg
    40741 tgatgcgcac gcttctttgc cgtttttcgg ccagccattc gagcaaaaca gcctcatcgg
    40801 acaggctgaa gggaagaaga atctcttccg gcagaccggc ttcgtcataa tactgccgca
    40861 gcgccgatga gagaatttct tcccggtccg cctttgtttt taccggcgta aaggatttgc
    40921 tgcccagcag ctttccttcg cgcacaaaca gaatgcagac ccggtgcgtt ccggaatcgg
    40981 aatgaacacc cagcacatcc tggtccgcgg cgccccccgc gtccacatgc tgccgctcca
    41041 gcgcgtgctc cagcgcgcca atgcggtcgc gcagcctcgc ggcgtcctcg tagcgcaagc
    41101 tctgcgccgc ttcactcatc tgctttttca aatcggaaat caattcgcgg cggcgacccc
    41161 gcagaaaaga tacggcgttt tccaccagcg tccggtatgc cgcttcgtca atccggtttt
    41221 tgcagggagc caggcaacgg ccgatttgaa attccaggca gggtctggcc cggagttgga
    41281 aatcccggtc ccggcacgtg cgcagaggaa agatgcgctg gatgaaacgg agcgtctccc
    41341 tggcggccag ccccgacggg tacggaccga agtaaagcgc cgcgtcattt tgccttttgc
    41401 gcaccagccg aaggcgcgga aaagcctccg aaggattcag cgacagatgg caataggttt
    41461 tgtcatcccg caggatgaca ttgtagcgcg ggcgatgctt cttaatgagg ttgttttcca
    41521 gaatcagcgc ttctttttcc gtgtccgttg tgatgaattc gatcgactga acgcgcgcca
    41581 tcaaaaacgg cgccatcggc ctagtgtccc tgcctgtcaa ataagaaccg acgcggcttt
    41641 tcagatcgtt ggccttgccc acatagatga ctttccgtcg cgcatccagc atgatataga
    41701 tgccgggcgc acgtggagcg gaagccatct tttcttttac agcggcattc tcatccatca
    41761 atacggattc ctcacccctc acccctcatc ccttgtccgc cccttatatt cctcgcagac
    41821 gtttcgcaga acatccggcc ggtttgtgaa aatgccgtcc acccccatct ccagcagccg
    41881 ccgcatgttc ttttcgtcgt taaccgtata gatatgcacc ggaatgccat ggcgtttcag
    41941 atccgccagc catgttccgt ttagttcgcg ccgcgaacag ttgaaggcgt caacgccaag
    42001 ggatagaaca atctccgagg gcagccggga gccatgttgc tttttttcgt acagcacggc
    42061 aacaggcgcc tccctgtcga tctgcttgaa atgcaaaatg gcgcggggat caaagctgga
    42121 atacaccaca tgctccttca tgccgatctg atcgaccatt ttcaggcatc gctcctcaat
    42181 acctccggaa acagcatccg tgaccgcctc cgtcttcacc tcgatgttga cggccatccg
    42241 gcccttgcag agcgccagga cttccaccag cgcaggaatg cgctctcccg cgtatttttt
    42301 gtgaaaccag acgccggcat cgagttggcg caggcgggca aacgggtagt cggcgaccat
    42361 tccccggccg tccgttaagc gcgataattt tgcatcgtga aagaccacca cttccctgtc
    42421 cgcggtgagc tgcacatcca gttccaccat gtcggccttc atctctattg ccgcccggaa
    42481 agcggataac gtgttttccg gacagcaggc cgacgccccg cggtgggcaa tgacggcaaa
    42541 cgcggggcat cccatcgctt cccggctggt tcgccggtca tcaggatagg tcagggcatg
    42601 atagggtatg gccatcggca gcaccgctta gcttttcagt gtccggtttt ctaacatatt
    42661 tgaccgcaga gttcttcaac tattttatcg tttacaaaat cccggagaaa aggtacaaag
    42721 cgagcaagga ttcaggggtc ccattccgac aaaccgggag gaaaacatga aagtacttgg
    42781 cattatggga agtgcgcgcg tcgggggaaa cagtgatgtt ctcctgagcc gcgctctgga
    42841 gggcgcccgg gaagccggag cgcgcgtgca aaaaatcgtc ctggaccgaa aacacattgc
    42901 cggatgccga gactgcaaga aatgcaacaa aaccggaatc tgcgtccttg cagacgacat
    42961 gtccggcatt caggagctga ttctggaggc tgacgcggtc cttcacgccg ttcccgttta
    43021 tttctggtcg atgagctcgc agatgaaggc ctacctggac cggtggtgcg ttttttttga
    43081 cgccgagtgg agatggcaga aagcctgcta ccccaaaatg cggggcaagc ggatcgggct
    43141 tctcactgtt tgcggggacc cggacccgca cacggccgac cccattgtcc acagcttccg
    43201 aatgaccgcg gaaatgacca aaatgcactg gctgggcgcc gtgatggcct ccgcatccga
    43261 caagggcgaa atcaagggca acggggccgc cctgcgggag gcgtttgatc tggggaaaaa
    43321 atccgccgcg ccgcagcagt gatttttcaa gcccgaagcc cgcgcgtcag ccttcgcatt
    43381 cggccggaat gaggcggatg cagaaaaagc tccccttccc cgcgggccga agattctccg
    43441 cttgcggcct cgaagacctt cccacgcgga tcagaagaat ttggccacca gatcggtgga
    43501 cgaccggcgg ccgattttgt cgaaatgatt tttgaggaat tcatcggcct tttcccggcc
    43561 ggtatcaaac aggtattgca gaaattccac atccagattc attttgctcg acgcggagag
    43621 ttttgccatt tcttcctcgg cgtcgatggt atggatgaag gtatgtttgt gtttggccga
    43681 atccagctcg cccttttcaa tcaaagacgt aataaaataa atagcccgca tttcccgcat
    43741 caggctggaa ttgaagctga gcgtgttgat ccggtcgaag atttcgttgg tgctgcgggg
    43801 aacctcggga atgttgatcg ggttgatctg gacgatcagg acgtcgcggc agtccgtgtc
    43861 gtaaatcacg ggaaaaatcg gcggattccc catgtagccg ccgtcccagt aatactggcc
    43921 gtcgatctcc ggcgcctgcg tcaggtaggg caggcaggcg gaggccatga tcatgtccgg
    43981 cgtgatttcg cgattttcaa aaaccttcag gcggccggtc aaaacattgg tggcgcagat
    44041 gaaaagccga atgctgcgcg acttctgcag cacggaaaag tcgatcagct cttcgagcat
    44101 ctccttgagg gcgtttttgt tgttggggtt ccactggtag ggggaaaaca tgcgcgaaag
    44161 aatgtcgaac atctgccagt acggagaaaa ttcaatcgac cctttgctgc ccatgagttt
    44221 gtcgaatacg gtcggctgga acggcgaaca ccggctgttc tttgagttcg tcatccagaa
    44281 acggttgagc agctccttcg ctttggcggg gcctcccgcc gccaggccat aggcgagaat
    44341 ggtcgcgttt acggcgccgg cgctcgtgcc gacaatgcct tcaatctcca ggcgcttttc
    44401 gtccagcagc cggtccagga ccccccaggt aaaggcgccg tgggagccgc cgccctgcag
    44461 ggctaaattc agttttttct ttttgggatg aaccggcaag ctcgctgacg atttcttcat
    44521 tcgattttac ccctgatcca cgatctaaaa gaaaagacat tcaagctttt taagggcctt
    44581 accaattccg catccgcttc cattgttcaa aacagcgcaa aaacatggta tccgtataac
    44641 acattttgga ataagatcaa tgattattgc ggcttaaatt attatgacac gccatccgcc
    44701 acgcgtttgg tatttcaaca ttcagttcgg gagtctttgt tgatttgcaa ttgtcgggtc
    44761 tcttattcat ttttcggcag gaaggatttt accgtaacct cgaagagtcc tttgtatgca
    44821 tctttcatct ctgcggggac agacaacttc agaacataaa atccctcgcc gcttccggcg
    44881 ggaatggtga caaatttttc ataaatcggc accttcgtcg ggttgataac ccgctctccg
    44941 atgaaccgga tgtctattct ctcaaatgtc ttcgcctgtc ttcccgcaac ctccatttcc
    45001 tgaattttac cgtaggattt tccctctatt tcaatgccca aattggatct ggagtgcctc
    45061 cggataaata tatccattgt gctgtgcaaa agattccccg gagcataata gtgaatggac
    45121 attaagggag caaccggact tgtgtttcgc ggacctatca ggataacgcc atatactttt
    45181 ttttcttcct cggataagcc aaagccaggg ggataaacgc tccagtcttc gggaatgctg
    45241 cattggaagt aatttccaaa agacgcatat tctttgaatt tttgtgcttt atccagcgcg
    45301 ctggaaactg cggaatgaaa ggtcatcacg acaatcatta caagaatccc tgataatcgt
    45361 ttcgtcataa aacctcctta aatcaataag cccggtcgtt gatcggactt ttctttaaca
    45421 ttcctgttgc tcttcaccgg ctgatattca ttcacttaaa acaaaacccc tgcggctcat
    45481 tttccaatca gcttgataaa atcaggatga tgttgaaggg aattgaatcc cgcctcgttg
    45541 cgaacattcg ccttataacc ggggtcgagt tgaatcgcat ttctcaagtc ctgaatgctc
    45601 ttgcgtgaat cccccttcaa cgcataggcc agagcgcggc cataataggc cgccgcggcc
    45661 tcccggggat taagcgcgat ggatctgccg tagtctttta tcgcctgatc gtacttgccg
    45721 attttgctgt aaaggtcggc tctttctctg aggtaaaacg gatcgtctgg ttttcgttta
    45781 agcaagttag tccaatcctg aattgccaga tcaaactttc caagcttctc atagctcatc
    45841 gctctcagca tataatcgtg agcggcaaaa agatggtccg gtccttccag ttcgatcgca
    45901 cggctcaaat cagcaaccgc cagttccggt ttctgaagca gttggtaggt ctgtccccgg
    45961 ccggaaaata gtcctgcgct tttcgggtcg agcctgatcg ctgcgttaaa atcctcaatc
    46021 gcaagatcat actgattgag gcccagataa gaatttgcgc gattatgaaa ataagatgag
    46081 tccgccggat cgagtgcgat ggcgctgctg aaatcccgga ccgagagctc aagcgcgccg
    46141 gggatatcct gtttggacgg tgctacgtaa ggtctggagc gaaacagcgt gtaatatctc
    46201 ccacggtcga aataagcgac tgccttttct ttgtttcctc gcgcctttgc gatcgctttc
    46261 ccgaactcct gaagcgcggg atcaagctga tcaagctcct tgtatgtcca ggcccggatg
    46321 agatgcgcct cggaatcctt cggttcgatg ctcaatattt tgtagtaatc ggacatggcc
    46381 tgattttttt cactgatccg cgcccggaaa taataagctt cgatgttctt cgggtcgagt
    46441 tcgatggcac ggctgtaagc atcgaaggcc cccttaaaat tgtcctcggg acctgaaacg
    46501 gcatgacctt tttcaatcca atccgccgcg gacagttccc tgatcgactg gtcataggcc
    46561 gccttctgcg actcccgacc cccgtttttc gccgaagcca tagccttgcg cagccgctca
    46621 acctcacgga gcaactcgtc ggatttctgt ttcatcgatt ccagctcttt tgtcttatct
    46681 cggtctttgc gcatggcgtc gatggactgg accactttat ccgaatcggc cacgattttc
    46741 gatttgagcc agtaaacgtg gccatcccat ttttccgcga taatttccgt ctggacgatg
    46801 ccggcagtca acgttgtgat ctggtctttt gtaagctgaa aattctttac ttccgtttca
    46861 ctttccaggt aagtccccaa ttcctccagc agcagccgct tcacttcgcg aagggcgatc
    46921 acccggctgg aatttttgct gtcctcatcg ccggcctgat aggtgtattc cttgatgaag
    46981 gttctggttt cggcaaaagc aacctggccc atcaacggga caaaaaaaca caaaaaaaga
    47041 gccattattt ttacagcatg tttcatcgat agtcctgcct cttaaagatc acaaatccgg
    47101 attctgtttt taatgcgctt cgtcccagtt agaccccgcc gcaatctcca cttttaacgg
    47161 caccttgagt ttcatcacgc cttccatttc ttttttaaca agcaccgtaa cgtcttcctt
    47221 ttcttcgacc ggagcctcaa ccaccagctc gtcatggact tgcataatca aacgggccga
    47281 cagatttttt tccgtcagga gacgatcaat gttgaccatc gccactttaa tcaaatcggc
    47341 ggcggagccc tggatgggcg cattgatcgc catccgttcg gcaaactggc gaacggatgg
    47401 aatccggctt tgcagctcgg gcaggtagcg gcggcgattc aggatcgtac agacaaagcc
    47461 gtcgcgcctt gcgccctcca gaatgccgtc cagatagtcg cggacttttt tatagcgggc
    47521 aaaatagccg tcaatgtagg cctgggcggt cttctgggaa atgcccagct ctctggccag
    47581 accgaacgcg ctcatgccgt aaagaacgcc gaagttgatg actttggcct gacgacgcat
    47641 atccggattg accatttcgg gaaaaacgcc gaagatgtcg gacgctgttc gcgtgtgaat
    47701 atcttcatct gcggcaaagg cttcgagcag cgttgcgtct tcggacaaat gcgcaagcac
    47761 ccgcagctcg atttgtgaat aatcggcgga gatcaaaaca caatccggcg ccgcgataaa
    47821 ggcctgacgg atccttttgc cttccattgt ccggatcgga atattctgca ggttggggtt
    47881 gctgctcgaa agccggccgg tcgccgccac cgtctggtta taggaggtgt ggattctgcc
    47941 ggtccgggga ttaaccaaaa cgggcagcgc atccatgtaa gtggacttca gtttggacaa
    48001 agaacgataa gccagaatct cggcgggcag ttcgtggctg cgggccagat ccgtcagcac
    48061 atcgacatcc gttgaatagc cttccttaat ttttttgccc ttcgggagtt tgagtttatc
    48121 aaaaagaatg gtctgcaatt gctttgggga gttgatgttg aatttttccc cggccaggcg
    48181 gtgaatcttg tcttcggaga tggacagcaa ctgccccaac tcatcagaca tctgctgcag
    48241 caggacggtg tccaccagaa cgcctttttt ctccattccc gccagcgtaa acagcagggg
    48301 catttccaca tcgcgataaa gcgatgcggc gcctgcttct tcgagtcggc tttccaggac
    48361 ctcggccaac gccatgatcg cttccgcgcg ctcgcccgca taggcggcca ttttatccac
    48421 gggcgccagc gacagagcat aggttttccc cttgccgccg gcgatgtcat ccggagtgct
    48481 gatgcgttcg tgaagatatt cccacgccat ttcatcaagc gcataggaat gcctggccgg
    48541 attgagcaga taagcggcca gttgtaaatc aaagccggcc cgaatcgtca gatccggcat
    48601 ggaaacaagt gttgttttca aatcgtaggc gtatttgaca atttcagcat tggcaaaaaa
    48661 agacgcaagc attgaaaagg tttccttggg atccaactgc tctttggcag gggtgtgtcc
    48721 cagcggcaga taaaacgagt cgccacccgc ccggaaagcc atgccgatta attcctgcgg
    48781 agggtttttc tcccgaatca tatcgcaggc gattttggag ttgttgtgca acccggctat
    48841 cagttccgaa agcgacttgc cgtccgccac gaccttgcag gctttaaccg gcttttcagc
    48901 ttttatttta atttgctgca aaagcgaaga aaattcaaat tcgccaaaga gcctggccag
    48961 cgtctccagg tccggcttcc tgcgcgccgc gtcttccaga tgaaaatcca tctcaatgtc
    49021 cgtgcgaatg agcgcgagct gccggctgag acgagcctga tccgcataat tgcggaaact
    49081 ctcgcgcagc ttcacgttgt gcagcttctg tgcgttttca atgacggcgt ccaccgaacc
    49141 gtactcctcg atcagccgcc gggccgtttt cggcccgatg cccggcacac cgggaatgtt
    49201 gtcggatgtg tcgcccgtca gtcccagaat ctccaccacc ttttccggcc ccacgccgaa
    49261 tttttcctcc acggccgccg catcgtaggt tttatctttc atcgtatcga ccatggtcac
    49321 ccggtcatcg ataagctgca tcaaatcctt gtcgccggaa atcatgaccg tgcgccaccc
    49381 tttttgacgg gccgctaccg caagtgtgcc gataaggtca tccgcttccg ttccctgctt
    49441 ttccagaaca cagattgaaa aaccgcgaat gacgtctttg atgaacggta cctgcggaat
    49501 cagatcatcc ggcatgggct tgcgcgtggc tttgtaatct tcaaattctc catggcgcgt
    49561 ggtcggccct ttgagatcaa aggtcacgac aatatagtcc ggactaagat ccctgagcag
    49621 cttcatcagc atggtggtga agccataaat ggcgttggtg ggaaagcctt tggagttggt
    49681 gagcgcagga atcccgtaat aggcgcggta tagataattg ctgccgtcta caattgccag
    49741 gaggggggtt tggttttttt cagccatcgc tcatcgtctc tacaggtcaa aggaagcgtc
    49801 aatcttaaag gttctgaccg cccgcatgtc cagcatatcc gtgacaccgg tcttcttctt
    49861 cacatccgac agaagggctt ggagctcttc ggtgttcgcg gcggtcacag tgaaccagac
    49921 attgtattcg tgcccgcggc ggtaattatg cgtcacgcct ttctcggcgc tgacgatgtg
    49981 aacaaatgat tgaagtttgt cctcgggcac ccgcgccgcg cacagagtgc tcgcccgccc
    50041 gagtttcttc ccgtcgaaca cggcgccgat cctccggatc acgccttttt ccttcattct
    50101 tcggacgcgg gccagcactt catcttcgct gattccacag cgctcgccga tgagccggaa
    50161 cggttcctct tccagcggaa attccttttg cagaaggttg agcagttttt tatcgatggc
    50221 gtccattttt attttcttcc tctttcgggc ttttcaaaat gtccagatac aaggcgcgca
    50281 agtcctgagg aatgagacgt atttttgtat acgtcgcaat gacgaaagac gcagcgcaac
    50341 gccgtagttg agcgtttttc aaaagcctgt cttcagcgct cccggttcat agagacacag
    50401 cggctcctcc gccagataat cgccggtggc ctcataggcc cgggcccggc agccgccgca
    50461 gactttgatg aactcgcagc gcccgcactt gcccttgtaa cggccgtaat cccgaagatt
    50521 gcgaaacact tcggagctct cccagatttc gccaaaactt tgtttccgaa cattgccgca
    50581 gtcgagctcc agataaccac agggctgcac ctgcccgaca tgcgaaataa agcaaaatgt
    50641 aatgcccccc aggcagcctc tggtcgattc atggaaatgt ccgcccgcat ttttcacggg
    50701 ctttgcacct ttgggcttgt tctggtgcat gatgcggaag taatgcggcg cgcaggtggc
    50761 cttgagctgg atttcgcacg tcaggctttc ccggtaaaac cagtggagcg tctcttcata
    50821 atcggcagcg gtgatggcct gctgcgccag atcttttccc cgtccggtgg gcaccagcag
    50881 gaagatgtgg tgcgccgccg cgccgacgcg aagcaccaga tcgtgaatgg ctttgatttg
    50941 acgaagattg gctgttgtaa tggtcgtatt gatttggaat tccatcccgg ccgatttcat
    51001 ggcttcgatg ccgcgcatcg cgcctgcaaa cgctccggct tcattgcgga aagcatcatg
    51061 gctttctgcg tcctggccgt cgatgctgac gctcacccgc tgaatgccgc tttccatcat
    51121 cttgcgcgca acggcctggt ccaccagcgt gccgttcgtc gccatgacca tgcgcaaacc
    51181 cttgcgggtt ccataggcgg caatatcaaa aatgtccgga cgcaaaagcg gctcgccgcc
    51241 ggtcaggatg atgactggct gcgacacccc cgcgatatcg tcaacgagac gcagacattc
    51301 gtcggtggag agttccccgg gataaggtcc cagatgtgac gcggcgcggc agtgcacgca
    51361 gttgagattg caactgcggg tcacttccca cgcaatcatg cgcacggaat tccccttccc
    51421 ctcgtgggac gcgtaccctt tagggaaggg gcgggacaag acacccgcat ccacgtccgt
    51481 tgcacgatca gaatctcccc tcccctcttg ggaggattga gggaaagcgc ccgcatctgc
    51541 gtccgtcgta cgattaaaat ctcccctccc cttgtgggag gggcaggggg aggggtgggg
    51601 ggagtgtgat aaacgctcca taatcgattc caatactcct tctatatttc ctaatatttc
    51661 attattccaa aaacgcaaca ccctgaaacc ttgagaggcc agccattgat cgcgctgtcc
    51721 gtcactttcc tgatgctccg cgtgctggct cccgtccaat tcaacaatca atttgtcctc
    51781 aaatgaaaca aaatcgacaa tgtaaccgcc gatcggttgc tgtctgcgaa actttgtgct
    51841 ttcgagctgc ctggatctga ggtgcttcca taataatcgt tccgcatccg ttgaattggc
    51901 tctgaggttt tttgccaatc gggtcagatt ggttttgtct tttttcaaaa gatttcaccc
    51961 tccccctgcc cctcccttca agggagggga gtctcccgga acatcctgcg atctaaccct
    52021 ctcccatcaa gggaggactg atcacccctc cccttgtgtg aaggggcggg gtgaggggga
    52081 atttccgcac gtcttttcgc gctccacatt tcacttcgcg agcgcctttg ccagatcctt
    52141 cgcccagtag gtgataataa tatcagcgcc ggcgcgtttg atggccgtga cgctctccat
    52201 catggctttt ccctcgtcga gccatcccag ctttgccgcg gctttgatta tcgaatattc
    52261 gccgcttacg ttgtaggcgg caagcggcaa atcgaattcc tgtttggccc ggcagatgat
    52321 gtccaggtac ggcagcgcgg gcttcaccat gataatgtcc gcgccttcat tgacgtcgag
    52381 cgagatttcc cgaatggcct cgtcggaatt ggccggatcc atctgatagg ctttgcggtc
    52441 gccgaactgc ggcgcgcttt cggccgcctc gcgaaacggg ccgtaaaacg aggaggcgta
    52501 cttggcggaa taggccatga tcggaatgtt ttccagcgcg ttttcatcga ggatctcgcg
    52561 aatggccgca atctgcccgt ccatcatggc cgacggcgcc accatgtcgg cgccggcttt
    52621 cacctgcgag agcgccgttt ccgccagtac ttccagcgtg gcgtcgtttt gcacctgccc
    52681 cttttcaata atgccgcagt ggccgtggct cgtatattcg cacaggcaga cgtccgtaat
    52741 caccagcatg tccggcactt cgttcttgac gcgcttcacg gcctgctgga cgatcccgtc
    52801 tttggcaaac gcgccggttc cctgctcatc cttcctggcc ggaatgccga aaagcagcag
    52861 cgcggggatg cccagcgcat gcgcctcgcg cgcttccttc gcaaggtggt ccaccgacat
    52921 ctgaaactgc cccggcatcg aggcaatggg ttttttcaca tccctgcccg gcacggcgaa
    52981 aagcggataa acgagctcgt ccgcgctcag gcgcgtttca cgaaccagtc tgcgaaagtt
    53041 ttcattcttt ctcaaccggc gtccccggta ttcaggaaat tgcatatttt actcctttgg
    53101 tgatgatatt tcctcgtcgg tcagatagca ggccggatcc ggcccccact cgtccccctc
    53161 cgcttcggcc cgcgcccgga agttcccgcc gcacacggaa agccaaacac atgccgcgca
    53221 gcgtccggta acgtattttt tcttatcctt cagtttcatg agaaactcgt ttttttcgtc
    53281 cgtccagatt tcgctgaagg gccgcttccg gacattgccc agcggccggt tgcgccagaa
    53341 ctgatcggga tacacttcgc cgtcccagct gatacagccg atgccgacgc cggtggaatt
    53401 accctcgttc atttccagga gctccaggat ttccgccgcg cgcgccgggt ccttctccct
    53461 cattttcaga tacaggtaag gcccgtcggc gtgattgtcc accgtgagga tttccggcgc
    53521 caggccgtcg tcaaacatgg ccttcgtctt atcggcgatg agatccagca ggcggcgggc
    53581 ctccccgtgc gtcaggtcct cctcgcggag cttcgagccg cggccggaat acaccagatg
    53641 gtaaaagcac atccgctcga tcttttcttt tctgagcagg tcgaacatgg ccggcacatc
    53701 ggccacgttg tggcggttga cggtgaagcg caggcccacc ttgatgcctg cttcccggca
    53761 gttgcgaatg ccttcgatgg ctttttcaaa cgcgcctttt ctgccgcgga aacggtcatg
    53821 cgtcggctgc aaaccgtcga ggctgacgcc gacatacgac aggccgattt ttttgagttc
    53881 ggcggcgatg tctttggtaa tgagcgtgcc gttggtagag atgaccgcgc gcatgccctt
    53941 gtccgtcgcg tactttgcca gttcaagcaa atccttacgc atcagcggtt cgccgccgga
    54001 aaacagcagg accggtgatc cgaaagccgc caggtcatcg atcatttttt tggcttcttg
    54061 ggtcgtcagc tcatcgggat agtcgatgtc ggccgagttg gaatagcagt gaatgcattt
    54121 gaggttgcag cggcgcgtca tgttccagac caccacaggt tttttgtccg ccgaaaattg
    54181 cagcagatgc gacggcagcg atcccgagtg ccggttgtag cgcagcacgt cggatggctc
    54241 caccgctccg cagtacagtt tggaaatgcc gatcatggac tgtcctttcc ttccggatgc
    54301 ttaaagtgat cgacgagcgc ccgaaccaga ccctccatcg tgtattcgtt ctggaggata
    54361 tcgattttga atcccgcttt cgcggcggtc ccggccgtta cgggaccgat gcaggccaca
    54421 cggacggagg gcggcagcgc aaaatccttt cccatgatgt ccacaaaatt cgtcacggtg
    54481 gaggagctgg taaacgtgat gacgtccacg tctcccgccg caagccgcac agccagttcc
    54541 tctttcttcc ggccggaatt caccgtccgg taggttgtgg ccacgacaac ttcggccccc
    54601 atctttttga ggccttcggg cagaagatca cgggctttgg ccgcgcgggg aatcagaatt
    54661 ttttggcctt gaatgttcag ggcggcaaag gattttaaaa taccttcggc gatgaactcc
    54721 tccggcacca gatccacacc gatgccgcgg gcctccaact gtcttgccgt agccgggccg
    54781 atgcagcaga ctttgacacc cttgaggtcg cgcatgtctt tgcccttttg ccgcaggcgc
    54841 tcgaaaaaga aatgcacgcc gttggcgctg gtgaaaatga tccactggta cgactccagt
    54901 tcctccaggg ccgcgtcgag ttcactccag ttggaaggcg gctcgatggc gatggttgga
    54961 aacgcgatcg gccgtgcgcc ttcagccgcc aaaagctttg ccagatcgtc ggcctgccgt
    55021 tcgggccgcg tgacaacgac gcccaggccg aagaggggct tcgaatcgaa ccaccccagc
    55081 gtatcgcgca attcgaccac cttccccacc accagaatgg cgggcggctt gaattggttg
    55141 gcgcgggcca gttcctcaat gttcgccagc gtgcccgtaa gggtttgctg ttcgggcagc
    55201 gtgccccggc gaatcaacgc agccggtgtc tccggagact ttccgcgcaa gatcaatgcg
    55261 tcggtgatct gaccaagatt tttcacgccc atcagaaaca ccagcgtccc gatgcccgca
    55321 agcgcctgcc agtcgatgtc gcttttttcc ttggtcggat cttcgtggcc ggtaataaac
    55381 gccaccgtgg aggtaagccc ccggtgcgtg agcggaatgc ccgcataggc cggcaccgcg
    55441 atggcggagg tcacgccggg aatcacttca aagggaatgc cggaagcggc cagctcttcc
    55501 gcttcctcgc cgccgcgccc aaagataaaa ggatcgccgc ccttgaggcg cgccactgtg
    55561 cggccgtcca gagcttcccg gaccagaagg tcgttgattt tgtcctgcgt cagtgtgtgg
    55621 tccccgccct gcttgcccgc gtatattctg cgtgcgccga ccggcgcgta ggccagaagc
    55681 tcttcattga ccagattgtc gtaaaccacc gcatcggcaa gcttcagggc ctccaccgcc
    55741 ctgagcgtga tgaggcccgg gtcccccggc cccgcaccga taatataaac tttacccttt
    55801 ttcattttac cccatcaact ccagcaggcg gcggccgcct ttttccagaa tcatctccgc
    55861 aagctttcgt cccatcgctt cggcttcgcc cggctcgccc cagaccttgt cgcgcagcac
    55921 ttccccgccg ttgggcgcgg ccagcaggcc ttccagcgtc agccgtttgc cttccatgag
    55981 gccgtaagcg gcaatcggca ggcggcagcc gccgcccagc gcccgcagat aggcgcgctc
    56041 cgccgtcact tccgcttcag ttacggcatg gttgagcctc gcgcacagcc ccgccagctc
    56101 cgcatcgctt tttcttacct gaagccccag agcgccctgg cccaccgccg gcagcatgat
    56161 ttccgtcggc agaaactgtg taaccacttc cccgtagccc agacgcttca cgcccgccgc
    56221 ggcaagaacc acgccggtga gattctccgt ttcgatcttt ttcaggcgcg tgtcaatgtt
    56281 gccgcgcagc ggaacaatct ccagatccgg cataaacgat ttcaactgcg cgccgcgccg
    56341 ctgggagccg gtcccgattt tggccccctt tggcaaatat tcaaatttaa tcctgccgcg
    56401 ggtcaccaga acatcgcgga cgtcctcgcg cggcagaatc gccgcaaaga cgagcccctc
    56461 cggagtctcg ccgggaacgt ctttcatgga atggattgcc aggtcaatcc gaccggcaag
    56521 aagcgcctct tcaatttctt ttacaaaaac cccctgcccg ccgatctgcg tcagagagac
    56581 gtcctgcata atatcgccgc tggtcctgat gacgcagatt tcagcctcga tctccggcgc
    56641 cgctttctgc agggcagcca ccgcggaatt cgtctgcacc agcgccagtt tactgcctct
    56701 tgttccgatt ttaatattca taaaactccc gattcatttt ttctccccgc cccttgcggg
    56761 aggggattaa ggggaggggg taagtttaat atccaacgaa catgacggct gtcagcaatt
    56821 tctccggcca aattcaccct ccctcaatcc ctcccctcga gggagggaag tcgcagggaa
    56881 aggtttcaaa ccgttccctg cattaatcat ccaatctaaa gagttgcctg gccgccgcca
    56941 cgatatcctg ggaggcaaat tcggcgcttt cctctttcat caccgcgacc ggcgcgtgca
    57001 gcaccttgtt gacaatgccg tgcaccaggg cttcgacttt ttcccggtct gcgttttcca
    57061 ggctttgcat ccagacggac gctttttcca tttccgcccg cacgatgttc tcagccttgc
    57121 tccgaagcga cacgatggtg ggcacggcct ccagctcctt gagccagctt acgtaattgg
    57181 ccgcctcttc ctcgatgatc gcttcggctt tcagcgcctc gcgacgccgg tttttgatat
    57241 tctcatcgac aatgttctgc aggtggtcga tgttgtacag atagacgttt tcaacgtcgt
    57301 ccgccgccgg a
//
LOCUS       DAOPAF010000004        48515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_847_length_48515_cov_48.820161, whole genome shotgun sequence.
ACCESSION   DAOPAF010000004 DAOPAF010000000
VERSION     DAOPAF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 48515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48515
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_847_length_48515_cov_48.820161"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1393
                     /locus_tag="PLQ77_00885"
     CDS             314..1393
                     /locus_tag="PLQ77_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL09267.1"
                     /translation="MNRVYERLRDRLETMASGYPATQNGVELKILQKLFSEEDAALFL
                     QMSPEPDTAQELALRLGAEVADTAARLEDMARRGLIFRIKAGEGIRYRPVPFIVGIYE
                     YQLNHLNLPLLKDISKYYLTGLGATFHGLKTPHLRSIPINTEIVADRPVFPYDDAASI
                     LRSKSRIAVAECFCRKAVRLYGKTCVHPAETCLQFDAFADYYVENGMARYIDTDEALA
                     ILRQSEAEGLVVHVLNSRQVEAMCCCCSCCCGMLISLKLFPAPALAVKSNYVGRFDEA
                     LCTQCGLCVERCTVDALGMKDEKIVFDAGRCIGCGLCVTVCSAGALNLDKKPEGSLYQ
                     PPETVFDTFAEMSKEKASQKGDGSI"
     gene            complement(1386..2087)
                     /locus_tag="PLQ77_00890"
     CDS             complement(1386..2087)
                     /locus_tag="PLQ77_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL09268.1"
                     /translation="MPRTARIAPKEFVYHILARGNNKQDIFKDEKDFKQYIDILLKYK
                     SKYNYKLYHYVLMTNHVHLVIEPTETGGELSQIMKGINLSYAQYFKKKFKHTGHFWQD
                     RFKSIIISKDEYLLACGSYVELNPVRAGMVKDPKDYPWSSYKANAYGEKNDLLDQHIF
                     YDKLATEETVRKEYRSFVQQMLKKNESMRGEMNRRVIYGGGDFTQRVNKRYKVDAVIK
                     KRGRPKKESADKPIK"
     gene            2278..3048
                     /locus_tag="PLQ77_00895"
     CDS             2278..3048
                     /locus_tag="PLQ77_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPL09269.1"
                     /translation="MKGLKICATCLFIAVLICFLFNIDGHAQRTIVVAVDSGDWAPMK
                     MKGAQGELAGFEIDMINAISSEAGFQVKIIEVGWEKIFDDLDQGKCDAIMSSVSITDK
                     RKEKFDFTQPYFSAEQLLVVPKALALSSLKGKTIAAFEATTGAIVLKKSELIEKKFYP
                     VGETQKAFNDLEDGKIDGIICDTPLAINYCFRRDAYLGKFAVASERTVLGKPSEKEEY
                     AMVVKKGNKDTLAVLNKGIKAVQAKDIDAGFKAKWIWW"
     gene            complement(3259..4257)
                     /locus_tag="PLQ77_00900"
     CDS             complement(3259..4257)
                     /locus_tag="PLQ77_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HPL09270.1"
                     /translation="MAGKTYKGLMVRETAENQFTRAIEELSTDGLPAGDVLVKVLFSS
                     LNYKDVLSAGGNRGVTKKYPHTPGIDAAGIVEESASPNFKAGDEVIVTSYDLGMNTFG
                     GLGQYIRVPVSWVVRKPASLSFRESMIFGTAGFTAALSILRLVEFGMDPADEILVTGA
                     SGGVGSIAVSILSHAGYNVTALNSLQDESAFLKKIGAKQVIEPAELVDASGRPLLKER
                     WTKCVDTLGGDVLSSAVRSIKGYGAITCCGNVISPDLKLTVFPFILRGVTLYGIDSQN
                     CPMPRRQKAWDMIAGQWKFPWLATLSSEVPLDGVTGVMDEIKAGSHRGRTVVNLWE"
     gene            complement(4315..5439)
                     /locus_tag="PLQ77_00905"
     CDS             complement(4315..5439)
                     /locus_tag="PLQ77_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09271.1"
                     /translation="MIKIGLLFLSFVFVFVVSGCGNYYKVVDPVSKKVYFTDSIDQKG
                     RGVIQFKDKLSKNVVTLPESEVMEITKDQYLAGAPVKAAQPIPYCTEDDADWCGPCWT
                     NPANTWCCKHRGGKDPNCGVDATTCKYCEGCHQGDDCINICAKPPKGAGGSTNIKITN
                     ATDKDVTIAFVTGASGGACKDVKKMISYQWVAQNTNWCKNPKQEGGPNAGYCTGIVPK
                     RSFVEVTRTGENALKCLTGSILLGGKLSCPEPTGFTQGEFTLNPTDTDTEAVDISLVN
                     GVNYALTVNLPGKAWKVQDGGAAVTSIGPNAGFDGDNNKNGIFPPGCTDCIVAVEGQI
                     PCPKITPNPKCQPKRICNIYRGGWTGGTVEYVISDLPKPK"
     gene            complement(5541..6647)
                     /gene="prfA"
                     /locus_tag="PLQ77_00910"
     CDS             complement(5541..6647)
                     /gene="prfA"
                     /locus_tag="PLQ77_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPL09272.1"
                     /translation="MGILLNKLHDIELRYRELEGMLSDAQMASRQGLYQKYAKEHSDL
                     SELVETIRRYEKIRTDLAENQGLLADSDEEVKALAKEEIPQLQQELAELEEKIKILLL
                     PKDPNDDKNVFLEIRAGTGGDEAGLFVGDLFRMYARYAESQGWKVEIVSSSPAGGMGG
                     FKEIIAQIEGRGAYSRLKYESGTHRVQRVPVTEAQGRIHTSAVTVAIMPEAEEVEVNI
                     DPNELRIDVYRSTGHGGQSVNTTDSAVRITHIPTGLVVTCQDEKSQLKNKAKALKVLR
                     SRLLDAMVQKQNAEQAQVRKNQVGSGDRSERIRTYNFPQGRITDHRINLTRYDLDSFI
                     NGNIGAVIDALASHHQAELLNKEGKGDTLKKLSF"
     gene            complement(6814..7056)
                     /gene="rpmE"
                     /locus_tag="PLQ77_00915"
     CDS             complement(6814..7056)
                     /gene="rpmE"
                     /locus_tag="PLQ77_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPL09273.1"
                     /translation="MKAGIHPDYKPTTITCVCGNVIETRSTKKDIKIEICSNCHPFMT
                     GKQKILDTAGRVEKFKKKYAPKAETAPAAKKTTKKA"
     gene            complement(7163..8410)
                     /gene="rho"
                     /locus_tag="PLQ77_00920"
     CDS             complement(7163..8410)
                     /gene="rho"
                     /locus_tag="PLQ77_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPL09274.1"
                     /translation="MNIEDIKRLPISELNNLAKELEVPGASGMRRQDLIFAILQTQAE
                     QNGVISGSGVLEILPDGFGFLRAVDYNYLPSPDDIYISPSQIRRFNLRTGDTISGEVR
                     PPKEGEKYFALLKVDEVNFESPESARDKILFDNLTPLYPQEKLNLEFDPENFSTRTLD
                     LFAPIGKGQRALIVSPPRAGKTVLLQDIAHSITANHKEVVLMVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRIADRRIFPAFDLIRSGTRKEELLIPKANLNRVWILRRLLQEMNPVDA
                     MEFIIGKIKKTETNQEFLDSMNS"
     gene            8762..10240
                     /locus_tag="PLQ77_00925"
     CDS             8762..10240
                     /locus_tag="PLQ77_00925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL09275.1"
                     /translation="MQEHNPEEGMEKSEVAGLGEICLEDLNPELKTACARAGWPSLVP
                     VQARTIPYLLAGRDVMIQSRTGSGKTGAYLLPIIQKINLAKNVAQALVLVPTRELALQ
                     VYRDSQVLAEGTGLKTAVVYGGVGYGPQLEAFRSGAQLVVGTPGRILDHLLKNTLTLD
                     HLRMLIFDEADRMLSMGFYPDMVKIRKYIPTGKIASSMFSATFPPQVIRLAGQFLREP
                     ELLSLSGNEVHIAESEHIYYVVPGMRKERSLVRIIEIENPASAIIFCNTKDTVHYLSV
                     VLKRFGYDADELSSDLAQTAREKVMARVRRGALRFLVATDVAARGIDIPDLSHVFQYE
                     PPEDPEAYIHRAGRTGRMGSSGVVISLVAEMERFKIQSIARFYTINMEERSLPGDEEL
                     ERVVSERITALLEARLRGRDNIQIERMQRFKPLARNLAQSEEELAIITMLLDDYYQQS
                     LHAPPVQPAADDAEAPGPTVQRPKPQRPNKRPRPGKRKEINP"
     gene            complement(10293..10700)
                     /locus_tag="PLQ77_00930"
     CDS             complement(10293..10700)
                     /locus_tag="PLQ77_00930"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HPL09276.1"
                     /translation="MLTPDFTRYSLALLEGDRLIHSSKGGGLRPLWDALEKYSEKSGL
                     ILHDKVMGLAAARLIHRSGIIAEVHTMVVSLPAVQFLKDHGIRLTAFHSAANILTRDK
                     SAVCPGEAIALGTGEPDAFYQKIREMMEIPVIA"
     gene            complement(10694..11188)
                     /locus_tag="PLQ77_00935"
     CDS             complement(10694..11188)
                     /locus_tag="PLQ77_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09277.1"
                     /translation="MQNPTISATGVASFRRAATAFGLLVFLPCLLSLINVTFAQSWKI
                     HFFPAAVFLAAVGFGPAGGLIAGITGSLYSAVALGNPWLIAGNALLGFLTGFFFRRTS
                     KIILSVLLAFAVQLPWLILSDYYFAGLSPLFIAKLVMVLFLGNLLWALLISLGMKPIK
                     KYLC"
     gene            11365..12318
                     /locus_tag="PLQ77_00940"
     CDS             11365..12318
                     /locus_tag="PLQ77_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPL09278.1"
                     /translation="MADATKFNRREILQKGLSLGLIAGGAVILGRPAFLSAKEKPAVI
                     PDLVAVKNGEPDALFKKAIELAGGMNQFVKKGQTVVVKPNIGFPRVPEVGATTNPLLI
                     KTIIESCWAAGARRVYVFDNVVTPTSGNARNCYKLSGIEEAAKSARGIIVPVDDFSYR
                     TVKIPGGKTLKTADVHSLILDCDVFINVPVLKHHSSAHLSIAMKNLMGIVKNRMEYHL
                     TGLDQCIADFCLYRRPDLNVVDAYRVLMSRGPAGPGNPRAADVQMKKTLLISKDIVAV
                     DAAAARIFGKDPEDIGYIKIAHAQKTGNMNLKELKIVTYTM"
     gene            12331..13863
                     /locus_tag="PLQ77_00945"
     CDS             12331..13863
                     /locus_tag="PLQ77_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL09279.1"
                     /translation="MTLVKIRKVISGIIFVLFVLLFLGPEKWSVFLSDVIAPFQFVPS
                     LVQTATAPSGLFMAGFIFIVLMTLIFGRVYCSFLCPLGTLQDLIIAGSRRLGLLPGHA
                     FAGPHNGLRYALLASTAAAAVIGSLSLVSLLDPYSLAGRFFSHFLQPLVVGAYNLGIA
                     ALKPFDVYWYPKQTAFVPLSVLAATAAFFLLILYLAATRGRLYCNTVCPVGTFLGLVA
                     RFSVFQLTVNPKACSECVRCENVCKAGCIDSHNAFVDMSRCIGCLNCLDACSQSAIRY
                     RRRQRQPHAGAWSPSRRGFVMGGIAAAAALLALNTKLRAWIDIRRAQAEPPVTPPGSR
                     DIAHFTRACTACSLCVSVCPTQVLTPAFMDFGASGLMQPKMNYEKSFCDYECSLCSRV
                     CPTGALASLSLEEKKLTQIGEAELLENICVVYVDHNNCGACGEVCPTHAIRFADKKNI
                     LYPEIDRQYCIGCGACQLACPTAPRSIVVHANPVHKKAEKYISPETTVGQKKPADDEF
                     PF"
     gene            13963..15009
                     /locus_tag="PLQ77_00950"
     CDS             13963..15009
                     /locus_tag="PLQ77_00950"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPL09280.1"
                     /translation="MKKKDLLKKPVEHIDIKKINAADIIRAMKKMSFSARDLASAAEL
                     YDRMLREKNCAVILTLAGSTSAAGCMQVYVDLVKNNMVDVIVATGASIVDMDFFEALG
                     FRHYQGTPFVDDKLLRSLYIDRIYDTFISEDDLQHCDQTIKKIADSLEPRPYSSREFI
                     AEMGRYLTKNAKKKNSLVQAAYENDVPIFCPAFSDSSAGFGLVLHQVEHPDNHLSIDS
                     VKDFRELTEIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEILGHDVTMHKYAVQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATTALPLLASYAYHKGSWKKRKKRALS
                     RIFAGDSGSRKKKL"
     gene            15221..16909
                     /locus_tag="PLQ77_00955"
     CDS             15221..16909
                     /locus_tag="PLQ77_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL09281.1"
                     /translation="MGNVYLERPWLKNYDKHVPHKLKYEEKSFTELFSEVVSRYPDKT
                     ALIYLGRSITFREVDILSNQIAHFLIRAGLKPDDVVGLHLPNLPAHYIAVLGAQKAGC
                     ITTGLSPLLTAHEMAHQLKDSDTKVIFTVDVLYSKVAEVAGKAPFSTVVVSEIADFLP
                     GVKRVLGKLLKKIPTAEVKPIAGKTVLRFMEIMKEMPKDPVAVRRSFDDTIFMMYTGG
                     TTGPAKGALLTQRSYMSNRQQMLAWVDLHAEDIALSAFPLFHIAGLALGGFSLTHGIT
                     QICVPNPRDTHFLIEALKKYRPTFVVNVPTVFFELLKKPDFKALDLKGHLKWCMSAAA
                     PFPAENIKELESIIGEGNFIELYGMTEMSPVMICNPLKGRKKPASVGMPFPDTEVQLL
                     DTATGNPVAPGEPGEIVIRGPQLMRGYFNKPEETANAVRDGWMHTGDVAKMDEDGYFY
                     LVDRVKDMVIVSGFKVFTRELDEILVTHRDVQMAASIGLADPERPGSERVGCAIVLHE
                     GVEKTDAKKEEIRAFLKERIAPYKMPRVIEFMDELPVSGVGKVLKRELKKMMTV"
     gene            complement(16940..18757)
                     /gene="typA"
                     /locus_tag="PLQ77_00960"
     CDS             complement(16940..18757)
                     /gene="typA"
                     /locus_tag="PLQ77_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPL09282.1"
                     /translation="MKKEHLRNVAIIAHVDHGKTTLLNAMLKQTGIFRSHQAVEDRVM
                     DSMALEKERGITIAAKNTAVEYQGVKINIVDTPGHADFGGEVERSLNMVDGVLLLVDA
                     SEGPLPQTRFVLKKALARHLPIILVINKIDRPDARVEEVISETYDLFIDLGAEENQIE
                     FPILYTNAKTGVSHAKRGDDSSDLQPLLQAILDAIPAPQGDDGANTQFLVTNLDYDPY
                     VGQLAVGRLQAGKLEMNKPYALLAKEKIISGVKLSALYTFYGLAKKPVTVAESGDIIS
                     LAGVENVTIGDTISSEDRPEALPRLTVDEPTVSMIFYVNDGPFAGREGKYLTSRHLLE
                     RLEKEALRNVAVRIKKLDRTDAFEVCGRGELQMAVLIETMRREGFEMTVSKPQVITRK
                     QDGKTLEPLERLLLDIPEEFVGRITEKLSIRKGRLESLVNKGSGRVSMEFLIPSRGLI
                     GFRGQFLTDTKGGGVMNSLLEDYAPWFGAIPQRSTGVLAADRAGRVTAYASFAMEDRG
                     EMLVAVGTDVYAGMIVGERNRPQDLNVNIVKEKKLTNMRASTSDATVILRPPRILSLD
                     QAIEFIAEDELVEVTPQSVRLRKMELDATKRQMKARREE"
     gene            19150..19590
                     /locus_tag="PLQ77_00965"
     CDS             19150..19590
                     /locus_tag="PLQ77_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL09283.1"
                     /translation="MTYIKINFGGNFEDEFQKAVDEVFHLVRPAFKNYECIWRPGVDV
                     CESADEVIVLADMAGLNKEELQIEVNRHKLKISGIRKALQLLQSARYRQAEIPHGYFE
                     RSIALPAPVDAQSSVASYADGILMVRMRKLPVNKAHRISVLSAP"
     gene            19624..22098
                     /gene="lon"
                     /locus_tag="PLQ77_00970"
     CDS             19624..22098
                     /gene="lon"
                     /locus_tag="PLQ77_00970"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPL09284.1"
                     /translation="MTEQNVSENKNTFNIPKVIPILPLYNVMVFPKTMIPLEVTGSAS
                     ALVDEAMTKDRLVGLIMARKEPDAAQPYTRDDLHAMGTCAVILKMAKTAENRTQLLLQ
                     GLSRFSIEELVGGKPYQQARIRLVDEKEVKDLETEALMANLLTLFDRILKLSPFLPPE
                     FGPMAKSITEAGTLADLITSVINAPVEEKQKVLDTADVKQRLKELTRMVNHQMEVLEL
                     GNKIQTKVKDDIDKNQREYYLRQQLKAIRQELGESDENTVETDEYRKKIEEKNLPGEA
                     KKEALRELERLSRMHPSSAEYTVSSTYLDWITMLPWHEAATDNLDIAAARKILDEDHY
                     GLEKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGHSIARALGRKFWRIALG
                     GVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKLGSDFRGDPSSALL
                     EVLDPQQNNTFTDHYLDVPFDLSKVLFIATANMLDTIPPALLDRLEVIELTGYTQEEK
                     MKIAERYLIPRQLAENGLSASQFKLTGKALSTIINGYTREAGVRNLEREIANACRGVA
                     AQIAEGSLKSKTVTEKDVHRYLGAVRVPTDIDARITKPGIAIGLAWTPVGGDMLFIEA
                     TAMKGKKGLTLTGQLGDVMKESVAAALSFLRTNAEELGVNPDFFEDRDIHIHVPAGAT
                     PKDGPSAGVTMLTALTSLLTSRKVRKQLAMTGEITLRGAVLPVGGIKEKVLAAYRAGI
                     KTLILPAWNRKDLEDIPASVQNKVKFHFVSDMMEVLTLALEPAKEKPAKQQAGKKAVK
                     PGSAKPAVKREKSKRTVARKPVLKKK"
     gene            22142..22318
                     /locus_tag="PLQ77_00975"
     CDS             22142..22318
                     /locus_tag="PLQ77_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09285.1"
                     /translation="MNEIFPGRICVLDRHAHGSQFVPEFGAVERDAARGFGHAVAVIG
                     RLPDVMEETGDFRF"
     gene            22367..22843
                     /locus_tag="PLQ77_00980"
     CDS             22367..22843
                     /locus_tag="PLQ77_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09286.1"
                     /translation="MEIAKTLYYRKRCSEAAEILRMVLSIHPTDLVARTLRMMLLRNM
                     ALDTPSHRTAAAVFRQATQEAENIQEHCDFHTEDFYCEYAIDMARRAEEENVGIYAFN
                     RAYSEMIQAGKFIEHMQKALKVIDEEVGGDLSARDDSEIITGPAHGRPVKLFTLNF"
     gene            22973..23359
                     /locus_tag="PLQ77_00985"
     CDS             22973..23359
                     /locus_tag="PLQ77_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPL09287.1"
                     /translation="MQKTIDYWIEESAEALQVAGHLFEKKDYSYALFFGHLAVEKMLK
                     AIYVAKTGEHAPQIHNLVRLAEKSGLSLHAARKDVLNRITAFNLEARYPDEKRSFRLR
                     CTEQFTSTELSNIKEILQWLKSIMKS"
     gene            23332..23652
                     /locus_tag="PLQ77_00990"
     CDS             23332..23652
                     /locus_tag="PLQ77_00990"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPL09288.1"
                     /translation="MAQVDHEIINTVQLFIRRLKEDGITVESAYLFGSHAVNRAGRWS
                     DIDVAVVSPDFSSDRFDERLRLMKLSTEIDTRIEPVPYHINDFQDTDPLVDEIKKCGI
                     RLTA"
     gene            23914..25251
                     /gene="pcnB"
                     /locus_tag="PLQ77_00995"
     CDS             23914..25251
                     /gene="pcnB"
                     /locus_tag="PLQ77_00995"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPL09289.1"
                     /translation="MLRAMQPLIIPRKEHRVSRKKLPPNVLRTLYRLRDNGFTAYLMG
                     GCVRDLLLERTPKDFDIATDARPGQIKRLFRNCRLVGRRFRLAHLHFADGILEVSTFR
                     ADALSDESDMERPVPNHHKPRHLKDDSGMVLRDNVFGTPEQDALRRDFTINALAYNIA
                     DFSVIDYTTGLADLRDGLIRPIGDPYVRFTEDPVRMLRAVRFAASHDFVIEPAAWETL
                     CTLSSTISRASPARLYEEMLKLFLLGAAGRAFGLLNISGLLAALFPMLSNWLGGNTQR
                     LNIMNANLETLDCRCRMGMPPSPALFFAAFFGPLLAETTLAGRRDGIPGRLTRDAACT
                     DLMKECSETVSIPGKVANQLRNILAMQHSLHSVPPRRPSVIVKRPEFADALAYLHLEG
                     TLGGENQSALEWWDAFLSGKSSSIIAETPADEASAKRRRRRKRRHRAKGTPVK"
     gene            complement(25334..26008)
                     /locus_tag="PLQ77_01000"
     CDS             complement(25334..26008)
                     /locus_tag="PLQ77_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPL09290.1"
                     /translation="MENRQKEGLEVSRREMLILSSGFVTALMLGNMPGAGLSHAAEIK
                     FPEGKCTGKMEKKVLVAYASKYGSTGGVADAVAKELCAKGVSADVTLIRNARNIGSYQ
                     GVVVGSAIYMGKWMPEAVDFVKKNKEALRRVPVAYFLVCMTLARPTDQKRAEVLAYMD
                     PVLQEVPEIKPVGLGTFAGAMHYGNLSWIYKKILQSKGTPEGDYRDWNALRSWAQEPV
                     YAKFFR"
     gene            complement(26096..26989)
                     /locus_tag="PLQ77_01005"
     CDS             complement(26096..26989)
                     /locus_tag="PLQ77_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09291.1"
                     /translation="MENIIYRGVLTKTAERSFLAGDNGSRHLEDEKIWSGYLTHWSGK
                     KVCARRLPQKDYDTGRPLLILWPDEPALTPPFVEIYFNERLVKYPLSFLGHLAVLADG
                     KVFNFSHWMNENEAMNPEEYFFRPALGEFAPDPASGRDNTENPQRPYYDKFGRLFMRT
                     IHVLRISGLDTRRLSGFFFAELEKIRGTPTDPKEPGKYRDFHILTRSCATIIRDGFHS
                     LGFEKIRGVFPRDLFVSMAYFFLKQHRLPNAQASLHTLRQLQVPEAAPSAMPPLLNPR
                     NRFRFRTLRKNIMPDTPGIYG"
     gene            complement(27030..28397)
                     /locus_tag="PLQ77_01010"
     CDS             complement(27030..28397)
                     /locus_tag="PLQ77_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPL09292.1"
                     /translation="MTTEADLSELKAILDERRVSAGQSVRHLHSRDESFHSPSLPEVV
                     VWPRSTEEVSRLVRWACRKKVPVTAWGAGTSLEGNPIPVRGGMVMDFSEMNRVLALLA
                     EDFQADVQPGVVYKELNRQLAREGLFFPPDPGAAATIGGMIGNNASGIRSVRYGATKD
                     YVMRLTVVLPGGDVIHAGNRARKSSSGYNLAALFTGSEGTLGIITEATLKLAGLPVNF
                     MAVRATFPAVRRATDTVYAIMRAGLSPVAMEFLDANVVHALNADRGLTLEENPTLLME
                     FSGYSEQGLSEEMSFVEEICFAKGALSLDRGIGAEERARLWEIRHQTYESVKRCHIGL
                     SPLIMDVAVPLSRYGEMVEFSKQEVRDLTAYLFGHAGDGNIHVHVMDNPQDPKRWARV
                     EEASRSIVMKALEFEGTCTGEHGVGIGKSRFMEREHGASLILMKRLKGLLDPENLLNP
                     GKFFL"
     gene            complement(28394..29074)
                     /locus_tag="PLQ77_01015"
     CDS             complement(28394..29074)
                     /locus_tag="PLQ77_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPL09293.1"
                     /translation="MAHTEFIERDAPAIAAFAKKAAAGAATPREKAVKLFYAVRDGIY
                     YDPYRIDPRREAFRATTILRQGYGYCVTKAVLLAALMRSQGIACRIHLADVRNHLTTK
                     RLKEMMKTDIFYNHGYNDVWLDGRWLKVTPTFNLSLCEKFKVKPLDWDGQSDAVLHPF
                     DMEGRRHMEYIHDRGSFDDLPFDFLVETFITRYGGSIEEFQRQTAKTGRFEEEAEGEA
                     KKVSGKSV"
     gene            complement(29093..30112)
                     /locus_tag="PLQ77_01020"
     CDS             complement(29093..30112)
                     /locus_tag="PLQ77_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPL09294.1"
                     /translation="MKAAVLHGVRDIRIQTVPDPVCAPHGVIVKVNACGVCGSDLHWY
                     KENGHEGAIFGHEFSGDIVEVGKNVQGIAAGTRCTAVGFSPCGKCFWCKQGKMHRCSD
                     MALLGYQFPGAMAEYVHVPFAIPGRSVFPLPEELSYEDGASVEPLSISCFSVNRGQPK
                     PSDIVAVIGLGVIGLYAVQILKALGVEKVLAAGRRPSRLAAAKRFGAHPVIDAAAEDT
                     LQAVMQATDQLGVNTVLECAGNQTTFDQAVAMARGGGKILLVGIYEEALHWQPLSVIG
                     KNLTLVGCLGGNFPAVIELLKTKKVSTENIVSHRFGLDQAAEAFRAQLQDPAAVKVMI
                     MPQER"
     gene            complement(30325..32085)
                     /locus_tag="PLQ77_01025"
     CDS             complement(30325..32085)
                     /locus_tag="PLQ77_01025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPL09295.1"
                     /translation="MILHPRTLHRIGPFILCLVLLWAITAQAGEAPPIRVGGAVPAVE
                     IASQSEYLLDYSGRLQYQDILSSGQPFQRHTKYSFQFSFKRATLWFKCRIAPADSGDE
                     TALASRRSFLVFDNAALGAVTLYVPVILDGKPDLIEFHGGWRQGEKSREFPFLYPTFV
                     LPDNLDASRPVVVRVATPYALQFRATLYTIDAFRKMSFVLFLIVGFFAGILIAMILYN
                     LALYLFIRDRQYLFYITYIGFLLLWQCVLVGLFQYIWPPLGAFLMSGIPVFAALMMIF
                     AVSFGIVFLNTPKTAPRHDKLLKGLAVFMGSIILMVLLRQLWLSNLLSYLSGQVVTVV
                     LFTAALSSLRSGFRPALFYLIAFGVFLLAAFVFFFKFYGLIPNNTFTMHIVIFGSAAE
                     AILLSFALGYRIRIMQEEEEQLRERERDLEAISVTDELTGLFNRRFLNPALVKKTAAA
                     RRSGTRLSLLMLDVDHFKDFNDTYGHPEGDKVLAALGRLLVQTLREEDIACRYGGEEF
                     VVILHNTDIRAALEVAERIRAGFEKISFQPGLSREIHATISIGAAELLTDENPEGLIS
                     RADQAMYQAKQTGRNRICRA"
     gene            complement(32125..33654)
                     /locus_tag="PLQ77_01030"
     CDS             complement(32125..33654)
                     /locus_tag="PLQ77_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPL09296.1"
                     /translation="MTEKKEDSRARRKTRKESMQMKAAGAWDIAFDAVNNFSLNGDAN
                     QAAAIALYAILSAIPLFILTLIAAGSFFSSNTTLQTDIIGAVQGVHPYFSEALLQQVG
                     QIEGKSKVLGWIGLLGLVWLSAAIFNAIESALNIIFRSRRKRNYFVSKLMAVAMIPAA
                     WLIGGLSVVITYLAALLAKEPSGWAGGVLFSLTMPAQIALRYLIPFLMVVLLVTFVYR
                     IIPTAKIRFSVVLAGSAIFAFLVEIAKQLFTWYLANYTRYNVIFGSLETVVILVIWVF
                     YVALIFLFCAEIMSSFERRDMLLLEKALLKPHDSLLKVDARLFQKFGRVYKRDEVVFR
                     ENDSGCEMFYVLSGRIRLERSDGHVKKTLAEMGPGQYFGEMAALIDVRRTATARALEN
                     SHLAVIDDRTFQNLIRESREVALAMLREFASRLKNSNAALEEFTHRRTQMLILLQILD
                     QPKATVDEHIEQISRLTRKEPSQIRNIFQELSGQGIVRLRDNRPDIDRDKMWSMFDSG
                     I"
     gene            complement(33778..34545)
                     /locus_tag="PLQ77_01035"
     CDS             complement(33778..34545)
                     /locus_tag="PLQ77_01035"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPL09297.1"
                     /translation="MPFFLRAPGSDTGVLLIHGLMAAPAEVREWAEFLHARGMTVYAP
                     RLAGHGTSAKDLLVRDYSDWLNSVDRGHAILKTCCRRILVAGFSTGAGLALQSAIGKP
                     HDFEAVIAVSAPLRFKSFSSRFAELLHGFNLCCSHVGLNRLAVEFVKNDADNPHINYL
                     LCPVSSFVQVKKLMRSVRRRLPDIQIPALILQGRHDPKVAPESGPAIFQRLGAHQKRF
                     AWIDFNRHGIVLGPAAREVFHEVESFLNDCRLTGSSK"
     gene            complement(34680..35327)
                     /locus_tag="PLQ77_01040"
     CDS             complement(34680..35327)
                     /locus_tag="PLQ77_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPL09298.1"
                     /translation="MKVKTQELFNFYSHFAGAVAAVVGTVYLAMAASDSASGLVTALI
                     YGISVVFLFSASTLYHAFKKEENELSFWRKMDRLAIFFMIAGTFTPICYFCLEDPYRW
                     WMIAFQWGLVGVGVISQIFFPGAPRKIYAIIYLSMGWSALFTIKQMLSNMSSSQAVLL
                     VSGGIAFTLGAVIYAVKKPRMIPGVFSFHELFHIMVLIGGVLHYAMIYGVYSRPV"
     gene            35507..37234
                     /locus_tag="PLQ77_01045"
     CDS             35507..37234
                     /locus_tag="PLQ77_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HPL09299.1"
                     /translation="MKTNASFSRFDRGVELHSLDRFGLRIKDARPSAARIFHFVLVLL
                     LLAAAACDRTPQTGSAGSKGDKPPAYGDILVRGDIGDASNLIPLLASDSASHAVGGMV
                     FNGLVKYDKNMNIVGDLAESWEISPDGLSITFHLRKGVRWHDGRPFTAADVLYTYQVT
                     VDPKTPTAYAGDFQKVKKALVLDDHTFRVTYDKPFAPALISWSSAVMPRHLLAGKDIA
                     QSPLSRHPVGTGPYRFKEWVPGQKIVLASNEDYFEGRPCLDGRITRIIPDTATMFLEL
                     RAQNIGMMGLTPLQYTRQTENNLFRQNFNKYRYLAFAYTYLGYNLKNPLFADRRVRQA
                     ISHAINKDEIISGVLLGLGKPATGPYKPGTWACNDKVKIYHYDPAKARELLKQAGWAD
                     ANSDGVLDRDGRSFEFEILTNQGNETRQKCAEIIQRQLAEVGIAAKIRVLEWSAFVTH
                     FINKRRFDAVILGWTIPPDPDAYDVWHSGKTAPEELNFVSYGNPEADEMLEKGRSTFN
                     RAERKKYYDRFQEILAEDQPYTFLYVPDDLIIISNRVRGIEPAPAGIGHNFIRWYVPK
                     DEQKYTMTR"
     gene            37355..37942
                     /gene="lexA"
                     /locus_tag="PLQ77_01050"
     CDS             37355..37942
                     /gene="lexA"
                     /locus_tag="PLQ77_01050"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPL09300.1"
                     /translation="MKKVRDIKSVEQSLVSFYRRQKRMPTYAEMMDLFEVRSKSVVSY
                     WIEKLIEKGILDKDAQGFLKLSGVSFGIPLVGNVAAGIPASAEEIPRDVLSMDEYLVT
                     RPESSFLLRVTGDSMIGAGIMEGDLVVVERERTPRNGDIVLAEVDGQWTMKYFRRRGG
                     KVVLEAANPAYAAICPKEELKIGGVITASVRKYLM"
     gene            37971..38198
                     /locus_tag="PLQ77_01055"
     CDS             37971..38198
                     /locus_tag="PLQ77_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09301.1"
                     /translation="MRAIISVSGWPCGGNGHRSVALSRKSFAAAMRKTPKTGRGPTMA
                     EIDRLISAAGAVALILSAFYFAPIVGSIFMK"
     gene            complement(38235..40685)
                     /locus_tag="PLQ77_01060"
     CDS             complement(38235..40685)
                     /locus_tag="PLQ77_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL09302.1"
                     /translation="MAKQIFIVDDNPNNLYLLASILKSRGWSVREAGNGKDALDAIRV
                     QPPDLIVSDILMPVMDGYALCRECKADPKLKSIPFVFYTATYTEPKDEDFALSLGADR
                     FLLKPQEPEILTGILEEFLHEGKAAPPEASSPLGEDMEFFRRHNEALFRKLEKKMQDL
                     EAANRELRMLEEIYRQSFEHVTDVIFTVDAGLRIISISPSVEKALGYKPREFIGRSIR
                     DLGYLFAADSMETALEHIGLVLQGTALPATIYEFRARDGAAKFGEVSGSPITRDGKVA
                     GLVAVARDITERRLAEQKLRENEKRYKLLTEKMTDIVWISDMNLKTLYVTPSVQSVLG
                     FSQEERMHQAVEQQITPESLSRGLEAMAAELALEKQALGDPTRLATLLLEYYHKDGST
                     RWMETIISGLRDDQGVLTGLHGVSRDVTKRKKAEEEKQKLEERLHRAEKMEALGKLAG
                     GVAHDLNNVLGVLSGYSELLLLELPEDHKARKNVEKILQSTEKGAVIIQDLLTLARRG
                     VAVSGIVNLNRIVDGFLKTPFFEKIKNDHPRITFRAECDPRLLNIKGSAVHLEKALIN
                     LVSNAAEAIAEAGEIVIRTENRYVDRILISHEEVQEGDYVLLIVSDTGMGIPAEHREK
                     IFEPFFTKKTMGWSGTGLGLPIVWGTVKDQNGYIDLQTKEGKGTTFILYFPVTREEAA
                     LPQQRKPLEDYRGGGERILVVDDIAEQREIASSLLMKLGYEVETASGGEEAVKFLQEK
                     EVDLVLLDMIMSPGPDGLETYRKIRDIDPRQKTILVSGFSETERVLTAQRLGAGAYVK
                     KPYVMETIGLAIRHELKR"
     gene            complement(40734..42308)
                     /locus_tag="PLQ77_01065"
     CDS             complement(40734..42308)
                     /locus_tag="PLQ77_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL09303.1"
                     /translation="MNQPSSPVEIDFNSEFQRAISLMEDTQKHILITGRAGTGKSTLL
                     KYFRDTTSKKAVILAPTGVAAVNVEGQTIHSFFHFKPNVTPASIKRRKKAEKDRLTLY
                     QKLTTIVIDEISMVRADLLDCVDKFLRFNGPSEKKPFGGVQMIFIGDLYQLPPVVSSA
                     EREIFKNHYPTPYFFSAKVFEALDIEYVELEKVYRQKDDEFVRLLNTIRNRSVTDDDL
                     AKFNSRCDPFFEAPSGSFYLALTSTNDLADTINEKRLAELPGKIWKAKGQIEGDFGKE
                     YLPTAVDLKLKKNAQIMMLNNDSFGQWINGTIGKIRKFEKDDEGDDVIVAELDNGDTA
                     RISPYTWKIYRFFIKDEELRSEEVGSFRQYPVRLAFAVTIHKSQGKTFENVVIDVGRG
                     TFAHGQMYVALSRCTTLDGIVLKKPLQKSHILMDWRVVKFLTNLQYARAAKTLSREDK
                     IKWIEEAIAEKKDIEILYLKSQDEKSRRTVRPLFVGKMEYKGYPYLGLEAICLERREK
                     RIFNVDKILEIGAEKR"
     gene            42480..42983
                     /locus_tag="PLQ77_01070"
     CDS             42480..42983
                     /locus_tag="PLQ77_01070"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPL09304.1"
                     /translation="MRKVWLTVLGGLLSVVFCMSVLAQTPPLSDGAQDSLIKSQVETA
                     VSLLAAIHARQQKGEMSADKAKELAAGLLRELRYGKDGYFWADTTEGVNVVLYGRKDT
                     EGRNRLNDRDAKGVFYVKEFLAKGKTGGYVEYRFTKQGQAASLPKRSYVLLFKPFGWV
                     VGTGYYR"
     gene            complement(43084..43539)
                     /locus_tag="PLQ77_01075"
     CDS             complement(43084..43539)
                     /locus_tag="PLQ77_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL09305.1"
                     /translation="MTQSEFKKIIANAIKAEVAANKFYSGVAKKAKDANLKKLFKELA
                     DEEAQHRLTLKGYAARGAEKFTFTESADYKVADELPTPPLTADLKPVDGLVIAIKNEL
                     AAMQMYTQLAKASADKAQKKVFQELASMERGHKKKLEDLYTNMAFPEVW"
     gene            43995..45359
                     /locus_tag="PLQ77_01080"
     CDS             43995..45359
                     /locus_tag="PLQ77_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPL09306.1"
                     /translation="MEQVKTLLEDQEIPRQWYNIMADLPTPMKPPLHPGTGQPVTPDD
                     LAAIFPMNIIEHEVSTQRWIDIPDEVLQKYLLWRPSPLCRARNFEKLLGCPVKIYYKN
                     EGVSPAGSHKPNSALPMAYYNKVAGTKRITTETGAGQWGSALAMACQMFGLECRVYMV
                     KISYGQKPYRRLMMNTWGAECIPSPSTLTQSGRDFLAKYPDSPGSLGVAISEAIEDAV
                     TSKVKSHYALGSVLNHVLIYQSIIGLEAQKQMQKLGIYPDVVMGCCGGGSNFAGIAAP
                     YVCDKIHGKQVKIVGAEPSSCPSMTKGPFAYDFGDLAQKTPLLPMYTLGHDYVPAPIH
                     AGGLRYHGMAPIVSHLVREKFIEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAV
                     VDEAMRAKEEGKEKVILFNWSGHGLVDMASYDAYLSGKLEAYELPDEEIQRALLAIKD
                     FPKP"
     gene            45462..45719
                     /locus_tag="PLQ77_01085"
     CDS             45462..45719
                     /locus_tag="PLQ77_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09307.1"
                     /translation="MKTPHDHKITRCPKLGDEMTFAYCLREAGNLPCARILFCWQAAF
                     DAASLLQENLTQEELKRFAEILPKDKVVSLIEILEKTKRQT"
     gene            45716..47080
                     /locus_tag="PLQ77_01090"
     CDS             45716..47080
                     /locus_tag="PLQ77_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPL09308.1"
                     /translation="MKNLDLFSQGTPADALETAPLAERMRPLTLADYIGQPHLVGEGT
                     LLRRSIAEDKLFSMIFWGPPGSGKTTLARILANETKANFVSFSAVLSGVKEIRQVIDD
                     ARDLWEAKKQKTILFVDEIHRFNKAQQDAFLPHVESGLVTLIGATTENPSFEVIAPLL
                     SRTRVMLLKQFAEEDLTGILKRALSDPQRGLGQFAIQIDDEAIKFIVGISDGDARTAL
                     NNLEAAASMVQSLPEEERMISAKSAEEALLKKSLLYDKDGEEHYNLISALHKSMRGSD
                     PDATLYWLGRMLAAGEDPLYILRRMVRFASEDVGNADPHALVLTMAAQQAFHFIGLPE
                     GELAIAQAAVYLAVAPKSNSLYTGYGKTKDLIHKTGYLPVPLHIRNAPTKLMKELEYG
                     KDYKYAHDYQDAFVPQEYFPEKLQGKVLYSPTDAGYEKIIRERVEEWRRRKNKAKKES
                     EKKG"
     gene            47540..>48515
                     /locus_tag="PLQ77_01095"
     CDS             47540..>48515
                     /locus_tag="PLQ77_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09309.1"
                     /translation="MRIFQKNENIVQRGTRPYNGSFLQPLAVGIVSAVLVALILVMGY
                     LDIRRNETNLVGFMEDQALTTISVLLRQTEDNLKSIAAAGGKMPSHLKTAKEAESAYS
                     IQLVYDAITKLGQNIDEQWKKKLLNNAYLRRFAEENHFWYVAVLNMRGDMIYQSGPLK
                     TDMLAKGDLIVNGRKATTIELLEKIRIRQDIGYAGVKLKRSGGIAIFCLDRKSRLYWS
                     LKVAVERAMSKMGEGHGIAYLQVFNDQKTLLGNIGDLPAGLTVSDLNVQEMMKGKKRI
                     LSRKVDRQGIKILDVAAPFLMGNPYLPKEKIAGIVRIGLDRGSMDKIIA"
BASE COUNT        10851 a        13168 c        13553 g        10943 t
ORIGIN      
        1 tggaaaaaag gggacggttc tatttaattt actcatcgac tgattctttc tttggccgtc
       61 cccttttctt gatcacgaat gcttcaatga accgaagata acctatttta tgattgcaat
      121 gaaggtcggg ttacactata aaaacgatgg ccgcccagaa acttttttga tccatcaata
      181 cagggcaaaa aaggagaaat tttgaaggca atggcaagga ttatcatggc ggtggccgca
      241 ggagagtatt gcaaaactat tcttattcgt cacgcaggcg aataccctaa caacctgaga
      301 ggttagtcag aaaatgaacc gtgtctatga aaggctgcgc gacaggctcg agacgatggc
      361 gagcggctat cccgcgacac aaaacggcgt cgaacttaaa attcttcaaa agcttttttc
      421 cgaagaggat gcggcgctgt ttctgcaaat gagccccgaa ccggatacgg cacaggaact
      481 ggctctgcgt ctgggtgccg aggttgcgga cacggcggca aggcttgaag acatggcccg
      541 tcgggggctg attttccgga tcaaagccgg cgaggggatc cgctatcgtc cggttccgtt
      601 catcgtcggc atttacgaat accagctgaa tcatttgaat ttgccgctgc tgaaagacat
      661 ttcaaagtat taccttaccg gattgggggc cacgtttcac ggtctgaaaa cgccccatct
      721 gcgttccatt ccgatcaaca ccgaaatcgt cgccgaccgg ccggttttcc cttacgacga
      781 cgcggcgtcc atcctcagga gcaaatcgcg gattgccgtg gccgaatgct tctgccgaaa
      841 ggccgtccgc ctgtacggga aaacctgcgt tcatcccgcg gaaacctgcc ttcagtttga
      901 cgcctttgca gactactacg tggaaaatgg catggcgcgc tacatcgaca ccgacgaggc
      961 gctggcgatc ctaaggcaga gcgaggcgga aggattggtg gtccatgtcc tgaattcccg
     1021 gcaggtggaa gcaatgtgct gctgctgttc ctgctgttgc ggcatgctga tttcgctcaa
     1081 gctttttccg gcgcccgccc tggccgtgaa aagcaactac gtgggtcgtt ttgacgaagc
     1141 gctctgcacg cagtgcgggt tgtgcgtcga aagatgcact gtagatgctc tgggaatgaa
     1201 agatgaaaaa atcgtttttg acgccggacg ctgcatcggc tgcgggcttt gcgttacggt
     1261 ctgttccgcc ggggcgctga atctggataa gaaaccggag ggcagcctgt accagcctcc
     1321 ggagacggtt tttgacacat ttgccgaaat gagcaaagaa aaagcctctc aaaaagggga
     1381 cggttctatt taattggttt gtcggctgat tctttcttcg gccgtccccg tttcttgatc
     1441 acggcatcaa ccttgtatcg cttgttgacc cgttgtgtaa aatcgccccc tccatagatt
     1501 actcttctat tcatctcgcc ccgcatcgat tcattcttct tcagcatctg ctgaacaaat
     1561 gaacggtatt cctttcttac ggtctcttca gtcgcgagct tgtcatagaa aatatgctga
     1621 tcgagcagat cgtttttctc cccgtaagca ttggccttat aactgctcca agggtagtct
     1681 tttgggtctt tcaccattcc agcccggacg ggatttaatt ccacatagct tccacaggcc
     1741 agcagatatt cgtccttgga aatgatgata cttttaaatc ggtcctgcca gaagtggccc
     1801 gtgtgtttga acttcttctt aaaatattga gcataggaca gattgatccc cttcatgatc
     1861 tgggaaagtt cgccgccggt ttccgtgggc tcaatgacca aatgaacatg gttggtcatc
     1921 aaaacatagt ggtacagctt atagttgtat tttgatttgt acttcaggag aatatcgatg
     1981 tattgcttaa aatctttttc gtctttgaaa atatcctgct tattatttcc acgagccaga
     2041 atatgataaa cgaattcttt cggggctatt cttgctgttc ttggcatggc tatcctatat
     2101 caaaacccca aatcgctgtc aataaataga accgtcccct ttttctgctt gacacgtttg
     2161 ccgcaatgag caaagaaaaa gcctctcaaa aaggggatac aaaaaaatat cgcagtggat
     2221 cggaataacg attttacgtt tagcatcatc gccaacattt tcagattagg gggttctatg
     2281 aaaggactaa aaatctgcgc gacctgtttg tttattgccg tattgatttg ctttctgttc
     2341 aatatcgatg gacatgccca gagaaccatt gttgttgccg tggatagcgg cgattgggct
     2401 cccatgaaga tgaaaggggc tcagggggag cttgctggat ttgaaattga catgattaac
     2461 gccatcagct ccgaagccgg atttcaggtt aagataatag aagtcggctg ggagaagatc
     2521 ttcgacgacc tggatcaggg gaaatgcgat gcgatcatgt cttcggtaag cataaccgac
     2581 aaacgaaaag aaaagtttga ttttacgcag ccatattttt ccgcagaaca gcttcttgtt
     2641 gtaccgaagg cactggcctt gtcctctctc aaggggaaga cgatcgctgc ctttgaggcg
     2701 acgaccgggg ctatcgtgct taaaaaatcc gaactcatag aaaagaaatt ctacccagtc
     2761 ggcgagacgc aaaaagcttt caatgatttg gaagacggca aaattgacgg tattatctgc
     2821 gatacccccc tggcgatcaa ctactgcttc agacgagatg cttacctagg aaaatttgct
     2881 gtcgcgagcg aaagaactgt tctgggcaag ccttcagaaa aagaggagta tgccatggtg
     2941 gtaaaaaaag gcaacaaaga cacgctcgct gtcctgaaca aaggaatcaa agccgttcaa
     3001 gccaaggata tcgatgccgg tttcaaggcc aagtggatat ggtggtaggg cttcctgtgt
     3061 agtgatgcag tataattgca gccgcgtcct tgaaagattt caggggcctt gtcagtgtca
     3121 agtgccataa atcaaaatgg ccaaaataga tgaatagatg gaaagttttc aggcaggggg
     3181 tggggcagcc ggccattcat gcgcccatgc ccctgccgcc ctgccggtgt tcagtgtttt
     3241 tcacaggcaa aattttaatc attcccacag attgaccacg gttctgcccc gatggcttcc
     3301 ggctttgatt tcatccatca cgccggttac gccgtccagg gggacctccg aagacagagt
     3361 tgcaagccag gggaacttcc actggccggc gatcatatcc caggccttct gacgccgcgg
     3421 catggggcag ttctgcgagt cgatgccgta gagcgtcacg ccgcgcagaa tgaacggaaa
     3481 gaccgtcagc ttgagatccg gcgaaatgac gttgccgcag caggtgatgg cgccgtatcc
     3541 tttgatggac cggacagccg acgaaaggac atcgccgccc agtgtatcga cgcatttggt
     3601 ccagcgctct ttcagcagcg gccgtccgct ggcatcgacc agctcggcgg gctcgatgac
     3661 ctgcttggcg ccgattttct tcagaaacgc gctttcgtcc tgaaggctgt tgagcgccgt
     3721 cacgttgtaa ccggcatgag aaagaatgga aaccgcaatg ctgcccacgc cgccgcttgc
     3781 gcccgtgacc agaatctcat cggccgggtc cattccgaat tccaccagcc gcagaatcga
     3841 aagcgccgcc gtgaatcccg ccgttccgaa aatcatgctt tcgcgaaacg acaggctcgc
     3901 cggttttctg accacccagg agaccggaac ccgaatgtac tggcccaggc cgccgaatgt
     3961 gttcatgccc agatcgtagc tcgtgacgat gacttcgtcg ccggctttga agttgggcga
     4021 agcgctttct tcgacaatgc ccgccgcgtc gatgccgggc gtgtggggat attttttcgt
     4081 cacgccccgg ttgccgccgg cggagagcac gtccttataa ttcagcgatg aaaaaagaac
     4141 cttgaccaga acatctcccg ccgggagccc gtcggtggat aattcttcaa tggcccttgt
     4201 gaactggttt tcggctgttt ctctgaccat cagccctttg tacgttttgc ctgccataat
     4261 gacctcctcg gaatggattg aaaagagtgg agtgcttgtc tgccgacccg tcaatcattt
     4321 cggtttcggc aaatcgctga tcacatactc cacagttcca cccgtccagc cgccgcgata
     4381 aatattgcag atgcgtttcg gctggcattt gggattgggc gttattttcg gacagggaat
     4441 ctgcccctct accgccacaa tacaatcggt gcatccggga ggaaatattc cattcttatt
     4501 gttgtccccg tcaaagcccg cattggggcc gatagaggtg acggcggcgc ccccgtcctg
     4561 aactttccat gccttgcccg gcaggttgac ggtcaaggca tagttcacgc cgtttaccag
     4621 actgatatcc acggcctccg tgtcggtatc cgtcggattc agcgtgaact ctccctgtgt
     4681 aaatccagtg ggctcgggac atgacaattt gccgcccagc aaaatcgagc cggtcagaca
     4741 tttcagggca ttttccccgg tccgcgtcac ttccacaaag cttcttttgg gaacgatccc
     4801 cgtgcagtat cctgcattag ggccgccttc ctgtttgggg tttttgcacc aatttgtgtt
     4861 ctgtgcaacc cattgatagg aaatcatttt tttgacgtcc ttgcatgcgc ctcccgatgc
     4921 gcccgtcaca aaggcgatcg tgacgtcttt gtcggttgcg ttggtgatct tgatgtttgt
     4981 cgatcctccg gcgcctttcg ggggcttggc gcaaatgttg atgcaatcat cgccctgatg
     5041 gcaaccttcg cagtacttgc acgttgtcgc atccacgccg caattcgggt ctttcccgcc
     5101 cctgtgcttg cagcaccagg tgtttgccgg attggtccag cacggaccgc accagtccgc
     5161 gtcgtcttcg gtgcagtacg gaatcggctg cgccgctttc acgggggcgc cggccaaata
     5221 ctgatctttt gtaatttcca taacttccga ttccggcaat gtcaccacat tttttgataa
     5281 tttatctttg aactggatga cgcctctccc tttttgatcg atgctgtccg tgaaatagac
     5341 ttttttggaa accggatcga caaccttata atagttgccg catccggaaa caacaaaaac
     5401 gaaaacaaac gacagaaaca ataacccaat tttaatcata aatgcatttc cctccatagg
     5461 ggacaccctt aagtatttaa gttaatcccc ttaattcttt tttatcttta cgctgcaatc
     5521 gacctgaaat ctgaccgctg ttagaaactt aatttcttaa gggtgtcccc tttaccctct
     5581 ttattgagca gttcggcctg gtgatgtgag gcgagcgcgt cgattacggc gccgatgttg
     5641 ccgttgatga agctgtcgag gtcgtagcgg gtgaggttga tccggtggtc ggtgatccgt
     5701 ccctgtggaa agttgtaggt gcggatgcgc tcggagcggt cgcccgaccc gacctggttt
     5761 ttacgcactt gggcctgctc ggcgttctgc ttttgcacca tggcgtccag aagacgggat
     5821 cttaatactt tcaaggcctt ggctttgttc ttgagctggg atttttcatc ctgacaggta
     5881 acgacaaggc ccgtcgggat gtgcgtaatg cggacggcgg aatccgtggt gttgacgctc
     5941 tggccgccgt ggccggtgga gcggtaaacg tcgatgcgaa gttcgtttgg gtcgatgttc
     6001 acctccactt cttccgcttc cggcataatg gcgacggtga cggcggaggt gtgaatgcgc
     6061 ccctgcgctt ccgtgacggg cacccgctga acgcgatgtg tgccgctttc gtatttcagg
     6121 cggctgtagg cgcccctgcc ttcgatctgg gcgatgattt ccttgaagcc gcccatgccg
     6181 cccgccggcg atgaactgac gatttccacc ttccagccct gggattccgc gtagcgcgcg
     6241 tacatgcgga acaaatcgcc gacaaacagg ccggcttcgt cgccgccggt gcccgcccgg
     6301 atttcaagaa agacgttttt gtcgtcgttg gggtctttgg gcagaagcag gattttaatt
     6361 ttttcttcca gttcggcaag ttcctgctga agctgcggaa tttcttcttt ggccagcgcc
     6421 tttacctctt cgtcgctgtc cgccagcagt ccctggtttt ccgcaaggtc tgtcctgatt
     6481 ttttcatatc tgcggatcgt ttccaccagt tcggagagat ccgagtgctc tttggcgtat
     6541 ttctgataca gtccctgcct ggaagccatc tgggcgtcgg aaagcatccc ttcgagctcc
     6601 cggtagcgga gttctatgtc atgaagtttg ttgagtaaaa tacccatata cttaatttat
     6661 atcgttgttt ttaatgcagg ttgtgaaggg aaggcgcatt gatagccctt ccccatgtct
     6721 gtgaggacgc aactataaat gccgggaaag ttttgatctg ttgatcatga gggcaaagtc
     6781 caaaagtgtc cgcctttcgg cagaaagtta agcctatgct ttttttgttg ttttctttgc
     6841 ggccggagct gtttcggctt tcggcgcata tttctttttg aatttttcca cgcgccccgc
     6901 cgtgtcgaga attttctgct tgccggtcat aaacggatgg cagttggagc agatttcgat
     6961 cttgatgtct tttttggtcg agcgcgtttc aatgacattg ccgcaaacac acgtgatggt
     7021 ggttggcttg tagtccggat gaatccctgc tttcattttc cttcttcctc ctgatcctct
     7081 atcggttgtt tggataaagg tctctgtaat tttcagcaga tgtgctgcct ataccataaa
     7141 ctttgaaaat tcaagataat tcttacgaat tcatggaatc cagaaattcc tggttggttt
     7201 ccgttttctt gatttttccg atgataaatt ccatcgcatc aaccggattc atttcctgca
     7261 gcagacgccg cagaatccag acgcggttga gattggcttt gggaatcagc agctcttcct
     7321 tgcgcgtgcc ggaccggatg aggtcgaagg cggggaagat acggcgatcg gcgatgcggc
     7381 ggtcgaggtg cagttccatg ttgccggtgc ccttgaactc ttcgaaaatc acttcgtcca
     7441 tgcggctgcc cgtgtcgatg agcgccgtgg agataatggt gagcgagccg ccgttttcga
     7501 tgttgcgcgc cgcgccgaaa aagcgcttcg gtttgtgaag ggcgttggaa tcgacgccgc
     7561 cggaaagcac cttgccggaa ggcggaacga ccgtgttgta ggcgcgggcc agccttgtaa
     7621 tcgagtcgag caggatgacg acgtcctttt tgtgctccac gaggcgcttg gctttttcaa
     7681 tcaccatttc agccacctgg acgtggcgcg acgcaggctc gtcaaaggtt gacgagatga
     7741 cttcgccttt cacgctgcgc tgcatgtcgg tgacctcttc gggacgctcg tcaatcagca
     7801 aaaccattaa aacgacttcc ttatgattgg ccgtgatgct gtgggcgatg tcctgcagca
     7861 gaacggtttt gccggcgcgc ggcggcgaga cgatgagcgc gcgctgtccc ttgccgatcg
     7921 gcgcgaacaa atccagcgtc cgggtggaaa aattttccgg atcaaattcc aggttgagtt
     7981 tttcctgggg ataaagaggg gtcaggttgt caaagaggat tttatcgcgg gccgactcgg
     8041 ggctttcaaa attgacttca tcaactttca gaagggcgaa atatttttca ccttctttgg
     8101 ggggacggac ttcgccggaa atggtgtcgc cggttcgcaa gttaaagcgg cggatctgtg
     8161 acggtgaaat gtagatgtcg tccgggctgg gcagatagtt gtaatcgacc gcgcgcagga
     8221 agccgaagcc gtccggaagg atttccaaaa cgccggagcc ggagatgacg ccgttttgtt
     8281 ccgcctgggt ttgcagaatg gcaaaaatca ggtcctggcg gcgcatgccg ctggctcccg
     8341 ggacttccag ctctttggcc agattgttga gctcgctgat gggcagcctc ttgatgtctt
     8401 cgatgttcat aaatttttcc ttgttgtaat gaatgacagg ctcgatattg aaggggtgaa
     8461 cgcgtgtgtc tgcattaatt ttccggaagc aaacgaggag gctcgcaatc ttaaagcagg
     8521 tacggattta atatcggctt gtttatttga atacttgaat cgctccaaca ggcagggctc
     8581 acagagcctc tgacgaattc gccgctacgc tatatgaaac atcggcgcct gtcaagggat
     8641 tttctgagac ccccgccttc gcggaataag cctcctgatc ctccaagttt tttcttgatt
     8701 cctgagccgc tctgaaataa gtgatattta taaattattt tgacgagagg atcatgaaat
     8761 catgcaagag cataatcccg aagaagggat ggaaaagtct gaagttgccg gtcttggcga
     8821 aatttgcctg gaggacctga atccggagtt gaaaacggcc tgcgcgcgcg cgggctggcc
     8881 gtcgctggtg ccggtgcagg ccaggaccat cccctacctg ctggccggcc gggatgtgat
     8941 gattcagtcg cgaaccggca gcggcaagac aggggcctac ctgctgccga tcatccagaa
     9001 gatcaatctt gcgaaaaatg tcgcccaggc gctggttctg gtgcccaccc gcgaactggc
     9061 cctgcaggtc tatcgggatt cgcaggtgct ggcggagggg accgggttga agacggcggt
     9121 ggtttatggc ggtgtcggct acggccccca gctcgaagca ttccggtcag gcgcccaact
     9181 cgtggtcggg acgccgggac gcattctcga tcatctgctc aagaacaccc tgacgctgga
     9241 ccatctgaga atgctgattt ttgacgaagc ggaccgaatg ctgtcgatgg gattttatcc
     9301 ggacatggtc aaaatccgca aatatattcc gacgggcaaa atcgcaagca gcatgttttc
     9361 cgccactttt ccgccgcagg tgattcgcct ggccggccag tttttgcgcg aacccgaact
     9421 gctgagctta agcggcaacg aggttcatat cgccgaaagc gagcacatct attacgtggt
     9481 gccgggcatg cgcaaggagc gctccctggt gaggatcatc gagattgaaa atcccgcctc
     9541 ggcgatcatt ttctgcaata ccaaggatac ggtgcactac ctgtccgtcg tgctgaagcg
     9601 cttcggctac gacgccgatg agctctcctc cgacctggcc cagaccgcgc gggaaaaggt
     9661 gatggctcgc gtgcggcgcg gggcgctgcg ctttctggtc gcgaccgacg tggcggcgcg
     9721 cggcatcgac attcccgatc tgtcccacgt ttttcagtat gagccgccgg aagatccgga
     9781 agcctacatt caccgggccg gacgcaccgg gcggatggga tcgagcggcg tggtcatttc
     9841 cctggtcgcg gaaatggaga gattcaagat tcagagcatc gcccgttttt acacgattaa
     9901 catggaagag cgttcactcc ccggcgacga ggagctggaa agggttgtct ccgagaggat
     9961 caccgcgctt ctggaggcgc gcctgcgcgg ccgcgacaat atccagatcg agcgcatgca
    10021 gcgttttaag ccgctggcgc gcaatctggc gcaatccgag gaagagctgg ccatcattac
    10081 catgctgctc gacgattact atcagcagtc cctgcacgcg ccgccggtgc agcccgcggc
    10141 ggatgatgct gaggcgccag gcccaaccgt ccagaggccc aaaccccagc gtccgaacaa
    10201 aaggccccgg cccggaaagc gaaaagaaat caatccatag ccgcaatcga ttgacttttc
    10261 ccaaagaagc agctcgggac tgtctccggc attcaggcaa tgacaggaat ctccatcatt
    10321 tccctgattt tttgataaaa ggcatccggt tcgcctgtgc ccagcgcaat cgcttcaccc
    10381 gggcagacgg cgcttttgtc gcgggtcagt atattggccg ccgaatggaa tgccgtcagg
    10441 cgaatgccgt ggtctttgag aaattgcacg gcgggcagcg acacgaccat cgtatgcact
    10501 tcggcaatga tgccggaacg atgaatcagt ctggcggcgg caagccccat gaccttgtca
    10561 tgcaggatca ggccggattt ttctgaatat ttttccagag cgtcccataa ggggcgcaga
    10621 ccgccgccct tggaagaatg aatcagtctg tcgccttcca gaagcgccag tgaatagcgg
    10681 gtaaaatcag gagtcagcat agatattttt taatgggctt catgccgagc gaaatcagca
    10741 aagcccacaa cagattgccc aaaaacaaga ccatgaccaa tttggcgata aacaggggtg
    10801 aaagaccggc aaaataatag tcggacagaa tcagccaggg aagctgaacg gcgaacgcca
    10861 gcagcacgga aagaataatt tttgaagtcc tgcgaaagaa aaaaccggtt aaaaatccca
    10921 ggagagcgtt gcccgcgatc agccaggggt tgccaagcgc gacggccgaa taaagagacc
    10981 ccgtgatgcc ggcgatcaga ccgcctgccg gaccgaagcc gacggccgcc aggaaaaccg
    11041 ccgccggaaa gaaatgaatc ttccagcttt gcgcaaacgt cacattgatg aggctcaaca
    11101 agcagggcaa aaacaccagc agaccgaaag ccgtggcggc tctgcgaaaa gatgcaacac
    11161 cggttgcgct tatcgtgggg ttttgcatga tcggttcatc ccttttcagt tttagcgatt
    11221 ctatactatt cacgggtctt tgaacagaaa aaattatcca ccgttttcaa ttgacacggc
    11281 ccggattttt ccctatattt ttccgcaaat accggcgcat cgcctttgtt cgtatcagca
    11341 tcgtccaatc aaacggaggc cggcatggca gacgcaacta aattcaaccg gcgggaaatc
    11401 cttcaaaaag ggctgtcgct cggtctgatc gcgggcgggg cggttattct gggccggccg
    11461 gcatttctgt cggcgaaaga aaaaccggcc gtcattcccg atctggtcgc cgtcaagaac
    11521 ggcgaacccg atgccctgtt caaaaaagcc attgaactcg cgggcggcat gaatcagttt
    11581 gtcaaaaaag ggcagacggt tgtcgtcaag cccaatatcg gttttccgcg cgtgccggaa
    11641 gtcggtgcca ccaccaatcc gcttctgatt aaaacgatca ttgaatcctg ctgggcggcg
    11701 ggcgccaggc gcgtttatgt ctttgacaac gtggtgacgc ccacctccgg caatgcccgc
    11761 aactgctaca agctcagcgg cattgaagag gcggcgaaat ccgcccgcgg catcattgtg
    11821 ccggtggatg atttcagcta ccgcaccgtc aaaatccccg gaggcaaaac gctcaagacg
    11881 gcggatgttc acagcctgat cctggattgc gacgtgttca tcaacgtgcc ggtgctcaag
    11941 catcacagtt ccgcgcatct gtccattgcc atgaagaacc tgatgggcat cgtcaaaaac
    12001 cgcatggaat atcatctgac cggcctggac cagtgcattg cggatttctg cctgtaccgc
    12061 aggcccgatc tcaacgtggt ggatgcctat cgcgtgctga tgagccgcgg gcccgcgggc
    12121 ccgggcaacc cgcgggccgc cgatgttcag atgaaaaaga ccctgctgat ctcgaaggat
    12181 atcgtggcgg tggacgccgc cgccgcccgg attttcggga aggatccgga agacatcggc
    12241 tacatcaaaa tcgcccatgc gcaaaaaacc ggcaatatga atttgaaaga gctcaaaatt
    12301 gtcacgtaca ccatgtagag gaccggcttc ttgaccctcg tcaaaatacg caaagtcatc
    12361 tccggcatca tctttgtctt gtttgtcctg ctgtttctcg gcccggaaaa atggtccgtc
    12421 ttcctgtccg acgtcattgc gccttttcag tttgtccctt ctctggtgca aaccgcgacg
    12481 gcgccgtcgg ggctgtttat ggccggcttc atcttcatcg tgctgatgac gctgattttc
    12541 ggcagggtgt attgttcctt cctgtgtccg ctgggaacgc ttcaggattt gatcatcgcg
    12601 ggctcccgaa ggctgggcct tctgcccggg cacgcttttg ccggacctca caacggcctg
    12661 cgctatgcgc tgctggcgtc aacggccgcg gcggcggtca tcggttccct gtcgctggtc
    12721 agtctgctgg atccctattc gcttgcggga agattttttt cgcattttct tcagccgctc
    12781 gtcgtcgggg catacaacct gggcattgcg gcgttaaagc cttttgacgt ttactggtat
    12841 ccgaaacaga cagccttcgt tcccctgtcg gttttggcgg cgaccgccgc tttttttctg
    12901 ctgattttgt atcttgccgc aacgcgcggg cggctctatt gcaatacggt ctgtccggta
    12961 ggaacgtttt taggactggt ggcgcgcttc tccgtttttc aattgacggt gaacccgaaa
    13021 gcctgctccg aatgcgtccg gtgtgaaaat gtgtgcaagg cggggtgcat tgattcgcac
    13081 aacgcctttg tcgacatgag ccgctgcatc ggctgcttga actgccttga cgcctgttcc
    13141 caatccgcca tccgctaccg gcgaagacaa cgtcagcccc atgccggcgc ctggtcgcct
    13201 tcgcggcggg gttttgtcat gggcggcatc gcggccgccg ccgctctttt ggcgttgaat
    13261 acgaaactgc gcgcctggat cgatatccgc cgggcccagg ccgagccgcc ggttacgccg
    13321 cccggatcac gcgatattgc acatttcacc cgggcgtgca ccgcctgttc tctgtgcgtc
    13381 agcgtctgcc cgacgcaggt tctgacgccg gcttttatgg atttcggcgc atccggtttg
    13441 atgcagccga aaatgaatta tgaaaaaagc ttttgtgatt atgaatgcag cctctgcagc
    13501 cgggtctgcc cgaccggcgc gctcgcgtcg ctttctctgg aggagaaaaa gctcacgcag
    13561 atcggcgaag ccgagctttt ggaaaatatt tgcgttgtct atgtcgatca taataattgc
    13621 ggcgcctgcg gcgaagtctg ccccacgcac gcgattcgtt tcgcggacaa gaaaaatatt
    13681 ctctatccgg aaatcgaccg gcaatactgc atcggctgcg gcgcgtgtca actggcctgc
    13741 ccaaccgcgc cccgctccat tgtcgttcac gcaaatccgg ttcataaaaa ggcggagaaa
    13801 tatatcagcc ccgaaacgac ggtcgggcaa aagaagccgg ccgatgatga atttcctttt
    13861 tgagcggaaa aaatcaacgg ctgtaatcga ttgactttta aggagccgtg atttaatagc
    13921 atattcaatt cgggaagaaa acacgggaga atgaaagaat tcatgaagaa gaaggatttg
    13981 ctgaagaagc ccgtcgagca catcgacatc aagaaaatca acgcggcgga catcatccgg
    14041 gccatgaaga aaatgtcctt ttccgcccgc gacctggcct cggcggccga actttacgac
    14101 cggatgctcc gggagaaaaa ctgcgcggtc attctgacgc tggccggctc caccagcgcg
    14161 gccggctgca tgcaggtgta tgtcgatctg gtgaaaaaca acatggtgga cgtgatcgtg
    14221 gcgaccggcg cgtcgattgt ggacatggat ttttttgaag ccctgggctt ccgtcactat
    14281 cagggaacgc cgttcgtgga cgacaagctg ctgcgcagcc tgtacatcga ccgcatttat
    14341 gacacgttca tcagcgaaga cgatttgcag cactgcgacc agaccatcaa aaaaatcgcc
    14401 gattcgctgg aaccccgccc ctattcgtcg cgggaattca tcgccgaaat gggcagatat
    14461 ctgacgaaga acgccaaaaa gaaaaattca ctggtgcagg cggcctatga aaacgatgtg
    14521 ccgatcttct gccccgcgtt ttcggacagc tccgccggtt tcggcctcgt gctgcatcag
    14581 gtggaacacc cggacaatca cctgtccatc gattccgtca aagatttccg ggagctgacc
    14641 gaaataaaaa tcaaggcagg caccaccggt cttttgatga tcggcggcgg cgtgccgaaa
    14701 aacttcgccc aggataccgt ggtctgcgcc gagattctcg ggcatgacgt gaccatgcac
    14761 aaatacgccg ttcagatcac cgtggcggac gtgcgcgacg gcgcgtgctc cagctcaacg
    14821 ctcaaggaag ccaattcctg gggcaaggtg gattcctcct gcgaacagat ggtgtacgcg
    14881 gaagccacca ccgctctgcc cctgctggcc agctacgcct accacaaggg aagctggaaa
    14941 aagcgcaaaa aacgggcgct gtcccggatt tttgcaggcg attccggaag ccgaaagaaa
    15001 aagttgtaaa tcctctgtta aaaaacggga cttcaaaaaa ccgttttctg atcattctcc
    15061 atgcagctgc aaagacgggc cgacccgtca ataagcggcc gcgcgtaacg ttccaccttt
    15121 tccgacgtac agggcaaatc tgtttttggc tacactgttt taagcgaagc atcgtcgcat
    15181 cagaaaccgt ttcttaccgc atttttgaaa ggagggttct atgggcaatg tttacctgga
    15241 aagaccgtgg ctgaaaaatt acgacaagca tgtgccgcac aagctgaagt acgaggagaa
    15301 aagcttcacc gagttattct cggaagtggt ctcccggtat ccggacaaga ccgctttgat
    15361 ttacctgggg cgatcgatca ccttcaggga agtggatatc ctctccaacc agattgccca
    15421 ttttctgatc cgggccggcc tgaaaccgga cgatgtcgtc ggcctgcatc tgcccaatct
    15481 tccggcgcac tatattgccg tgctgggggc gcaaaaggcc ggatgcatta ccacgggcct
    15541 gtctccgctc ctgacggcgc atgagatggc gcaccagctt aaagattcgg acacgaaagt
    15601 catcttcacg gtggacgtgc tctattccaa ggtggcggaa gtggccggaa aagccccttt
    15661 ttcaacggtg gtcgtgtcgg aaattgccga ctttctgccg ggcgtgaaac gcgtgctggg
    15721 gaagcttttg aaaaagatcc ccacggcgga ggtgaagccc atcgccggaa aaaccgtttt
    15781 gcgctttatg gagatcatga aagaaatgcc caaagatccc gtggcggtca ggcgatcgtt
    15841 cgacgacacc atcttcatga tgtacacggg aggcaccacc ggcccggcca agggcgccct
    15901 gcttacccag cgaagctaca tgagcaatcg tcagcagatg ctggcgtggg tcgatcttca
    15961 tgccgaagac attgcccttt cggcatttcc tctgtttcat atcgcggggc ttgccctggg
    16021 cggtttttcg ctcacgcatg gaatcaccca gatctgcgtg cccaatcccc gcgatacgca
    16081 cttcctgatc gaagcgctga aaaaataccg tccgaccttt gtcgtgaacg ttcccacggt
    16141 cttttttgaa ttgttgaaaa agcccgattt caaagcgctc gatctcaagg gacacctcaa
    16201 gtggtgcatg agcgcagccg cgccgtttcc ggcggagaac atcaaagagc tggaatcgat
    16261 tatcggtgag ggaaatttca tcgaactcta cgggatgacg gagatgtcgc cggtgatgat
    16321 ctgcaatccc ctcaagggca ggaaaaaacc ggcgtccgtc ggcatgccgt ttcccgacac
    16381 cgaagttcaa ctcctggata cggcgaccgg gaaccccgta gcgccgggcg aaccgggcga
    16441 gatcgtcatc cgcgggccgc agctcatgag gggttatttc aacaagcccg aagaaaccgc
    16501 caacgccgtt cgggacgggt ggatgcatac cggggacgtg gcgaaaatgg acgaggacgg
    16561 ttatttttac ctggtggacc gggtaaaaga catggtgatc gtgtccggat tcaaggtttt
    16621 cacccgggag ctggacgaga ttctggtgac gcaccgggat gtgcagatgg cggctagcat
    16681 cggcctggcc gatccggagc gtccgggctc ggagcgcgtc ggctgcgcca tcgttctgca
    16741 tgaaggcgtg gaaaaaaccg atgcgaaaaa ggaagagatc cgcgcctttc tcaaggaacg
    16801 gatagcgccc tacaagatgc cgcgcgtcat cgagttcatg gacgagcttc ccgtaagcgg
    16861 cgtgggcaag gtgctcaagc gcgaattgaa aaagatgatg acggtgtagg accgctcgcg
    16921 gcggcgcaag cccgcgggcc tactcctccc ggcgggcttt catctgacgc ttcgtcgcgt
    16981 ccagctccat cttgcgcagg cgaacgcttt gcggggtcac ttccaccagt tcgtcttcgg
    17041 cgatgaactc gatggcctga tcgagcgaca gaatccgcgg cggccgcagg atcacggtgg
    17101 cgtccgaggt ggaagcccgc atattggtga gcttcttttc cttgacgatg ttgacgttga
    17161 ggtcctgcgg gcggttgcgc tcgcccacga tcatgcccgc gtaaacatcc gtgccgacgg
    17221 ccacgagcat ctccccgcgg tcttccatcg caaagctggc gtatgccgtc acgcggcccg
    17281 cgcggtcggc cgccagcacg cccgtgctgc gctggggaat ggcgccgaac cacggcgcat
    17341 agtcttccag cagggaattc atcacgccgc cgcctttcgt gtcggtcaga aactggccgc
    17401 ggaagccgat gaggccccgc gagggaatca gaaattccat gctgacgcgc ccgctgccct
    17461 tgttgacgag gctttccagt cttcccttgc gaatggaaag cttttccgtg atgcggccca
    17521 cgaactcttc ggggatgtcg agcagcagac gctccaaggg ttcaagcgtt tttccgtcct
    17581 gctttcgggt gatgacctgc ggcttggaga cggtcatctc gaatccctct cgtcgcatgg
    17641 tttcaatcag tacggccatc tgcagttcgc cccgtccgca cacttcaaac gcgtcggtgc
    17701 ggtccagttt tttgatccgg accgcaacgt tgcgcagcgc ttccttttcg agcctttcca
    17761 gaaggtggcg ggacgtcagg tatttgcctt cccggccggc aaacggcccg tcgttgacgt
    17821 agaagatcat ggaaacggtc ggttcgtcca ccgtcagccg gggcagggcc tccggacggt
    17881 cctccgatga aatcgtgtcg ccgatggtga cgttttccac gccggccaga gagataatgt
    17941 cgcccgactc cgcaacggtg accggctttt tggccagtcc gtagaaggta taaagggcgg
    18001 acagcttgac gccgcttatg atcttctctt ttgccaagag ggcatagggc ttgttcattt
    18061 ccagttttcc ggcctgcagg cggccgacgg ccagttgacc gacatacgga tcataatcca
    18121 gattcgtcac taaaaattgc gtattggcgc cgtcgtctcc ctgaggcgcg ggaatcgcgt
    18181 ccagaatggc ctgcagcagc ggctgcaaat cggaactgtc gtcgccgcgt ttggcgtggc
    18241 tgacgccggt cttggcattg gtgtacagaa tggggaactc aatctgattt tcttcggccc
    18301 ccagatcgat gaagaggtcg taggtttcgg agatgacttc ttccacgcgc gcgtccggcc
    18361 ggtcgatctt gttgatgacg agaatgatgg gcagatgacg cgccagggct tttttcagga
    18421 caaagcgggt ctggggcaga gggccttcgc tcgcgtccac cagcagcagg acgccgtcca
    18481 ccatgttgag gctgcgttcg acttcgcctc cgaaatcggc atggccgggg gtgtcgacga
    18541 tgttgatctt gacgccctga tattccacgg ccgtgttttt cgccgcgatg gtgatgccgc
    18601 gttccttttc cagcgccatc gagtccatca cgcggtcttc caccgcctgg tgggagcgga
    18661 aaatcccggt ctgcttgagc atggcgttga gcagcgtggt ttttccgtga tcgacatggg
    18721 caatgatcgc gacatttctc aagtgttctt ttttcatttt tctctttcta tccggtcgtc
    18781 aatgggaagg tgttttcgaa ctgaacgaat cgaatcgcaa ccgctgaaat gtcccgcctg
    18841 actgtttttt gggcgagtga gctctcccat agcaaaatat cgccgattaa accactgatt
    18901 ttttttcagg tttcctctcg tatccctggc ttaaactatt ccctgcgttt gttgacgcac
    18961 tcaccgtgaa aagaacagtg aaaagcaacg agtggaaagc gcaaagagga aaaactcatc
    19021 gctcattgct catcactttt ccctatccac cattcaccat ccactatttt cccccgggcc
    19081 tttacaaatc cgctttgaat cactatactt tttacgcagt caaaaaatcg catttgagag
    19141 gcacaggaaa tgacctacat caaaatcaat tttggcggta attttgaaga cgaattccag
    19201 aaagcggtgg atgaagtctt tcacctggtt cgcccggcct ttaaaaatta tgaatgcatc
    19261 tggcgccccg gcgttgatgt gtgcgaatcg gcggacgaag tgatcgtgct ggccgacatg
    19321 gcggggctga acaaggaaga actgcagatc gaagtcaatc gccacaagct caaaatatcc
    19381 ggcattcgca aggcgcttca gcttctgcaa agcgcccgct accgccaggc ggaaattccg
    19441 cacgggtatt tcgaaagaag cattgccctg ccggcgcccg tggacgcgca gtcctccgtt
    19501 gcctcttacg ccgacggcat tctgatggtg cggatgcgca agctgccggt caacaaggcg
    19561 caccggatct cggtcctttc ggcgccctag cggcttttcg ccctacgaca acaggaggtt
    19621 accatgacgg aacaaaatgt ttctgaaaat aaaaacacgt tcaatatccc gaaagtgatt
    19681 cccatcctgc cgctttacaa cgtgatggtg tttcccaaaa cgatgattcc gctggaggtg
    19741 acgggcagcg cctcggcgct ggtggatgag gccatgacca aagaccggct ggtggggctg
    19801 atcatggcca gaaaggagcc ggatgccgcc cagccgtaca cccgggacga cctgcatgcg
    19861 atgggcacct gcgcggtgat tctgaagatg gccaagacgg cggaaaaccg aacgcagctt
    19921 ctattgcagg ggctcagccg tttttccatc gaagagcttg tcggggggaa gccctaccag
    19981 caggcgcgca ttcggctggt ggacgaaaag gaagtcaagg atctggaaac cgaggcgctg
    20041 atggcgaatc tgctgacgct gtttgaccgg attttgaagc tttcgccgtt tctgccgccg
    20101 gaattcgggc cgatggccaa gtccatcacc gaagcgggaa cgctggcgga tttgattacc
    20161 tccgtcatca acgcgccggt ggaggagaag cagaaggtgc tggatacggc cgacgtcaaa
    20221 cagcgcctca aagagctgac ccgaatggtg aaccaccaga tggaagtctt ggaactgggc
    20281 aacaagattc aaaccaaggt gaaggacgac atcgacaaga accagcggga atattatctg
    20341 cgccagcagc tcaaggccat ccgccaggag ctgggggaaa gcgatgaaaa cacggtggag
    20401 acggacgagt accgcaaaaa aatagaagag aaaaacctgc ccggggaagc caaaaaagag
    20461 gcgttgcgcg agctggagag gctttcgcgc atgcacccgt cctcggcgga gtacacggtt
    20521 tcctccacgt acctcgactg gatcaccatg ctgccgtggc atgaagccgc gacggacaac
    20581 ctggacatcg ccgccgcgcg caaaattctg gacgaggatc attacgggct tgaaaagccc
    20641 aaaaagcgga tcattgaata cctggcggtc cgcaagctca agccggattc caagggtccg
    20701 atcctctgct tcgtggggcc tccgggcacc ggcaaaacct cgctggggca ttccatcgcg
    20761 cgggcgctgg ggcgcaagtt ctggcgcatc gcgctgggcg gcgtccgcga cgaagcggaa
    20821 atccgcggcc atcgacgcac ctacatcggc gcattgccgg gccggatcat tcagggcctg
    20881 aggcgcgcgg agtcgaacaa tcccgtgttc atgctcgatg agatcgacaa gcttggaagc
    20941 gacttccgcg gcgatccctc ttccgcgctc ctggaggtgc tggacccgca gcagaacaac
    21001 accttcaccg accattacct ggatgtgccg ttcgatttgt cgaaagtgct gttcatcgcc
    21061 acggccaaca tgcttgacac gattccgcca gcgcttctgg accgtctgga ggtgatcgaa
    21121 ctgaccggct acacgcagga agaaaagatg aagatcgcag agcggtatct cattccgcgg
    21181 cagcttgcgg aaaacggcct ttccgcttcc cagttcaaac tgacgggcaa ggcgctttcc
    21241 acgatcatca acggctacac gcgggaagcg ggcgtgcgca acctggagcg ggagatcgcc
    21301 aacgcctgcc gcggcgtggc ggcgcagatt gccgaaggga gcctcaagtc caaaaccgtg
    21361 acggagaaag acgtgcaccg gtatctggga gcggtgcgcg tgccgacgga catcgatgcg
    21421 cggatcacga aaccgggcat cgccatcggt ttggcctgga cgcccgtggg cggcgacatg
    21481 cttttcatcg aagccaccgc catgaagggc aagaaggggc tcacgctgac cgggcagctg
    21541 ggcgacgtca tgaaggaatc cgtcgcggcg gcgctgagct ttctgcgcac caacgcagaa
    21601 gagctgggcg tgaatcccga ttttttcgaa gaccgcgaca ttcacattca cgttccggcg
    21661 ggcgcgacgc ccaaggacgg tccgtcggcg ggcgtcacga tgctgacggc gctcacctcg
    21721 cttctgacca gccgcaaggt gagaaagcaa ctggcgatga ccggagagat cacgctgcgc
    21781 ggcgcggtgc tgcccgtggg cggcatcaag gaaaaggtgc tcgcggccta tcgcgcggga
    21841 atcaaaaccc tcattctgcc cgcgtggaac cggaaggacc tcgaggatat tccggccagc
    21901 gtgcagaata aggtgaaatt ccattttgtg agcgacatga tggaggttct cacgctggcg
    21961 ctggagccgg ccaaggaaaa accagcaaaa cagcaggcag ggaagaaagc ggtgaagccc
    22021 gggagcgcaa agccggcggt gaaaagggaa aaaagcaaaa ggacggttgc gcgcaaaccg
    22081 gttttaaaaa agaaatgatt ttcctgatcc cctcggcaaa acctgagggg agtctcgtgc
    22141 tttgaacgaa atttttcccg gccgtatttg tgtgctggat cggcatgccc acggttccca
    22201 atttgttccc gaatttggtg cagttgagcg tgacgccgcc cgaggtttcg gtcatgccgt
    22261 agcagtcata ggtcggctgc ccgatgtcat ggaagaaacg ggcgatttcc ggttttaaca
    22321 gcgagctgga gctgacgatg aatttcagat tgccgccgaa aatggcgtgg agattgccaa
    22381 gacgctctat taccgcaaac gctgttcgga agcggcggaa attctccgga tggttttatc
    22441 cattcatccg acagatctgg tggcgcggac gctgcggatg atgctcttga ggaatatggc
    22501 gctggatacg ccttctcacc gaacggcggc cgctgttttt cggcaggcca cccaggaagc
    22561 ggaaaacatc caggagcatt gcgatttcca tacggaagat ttctactgcg aatacgccat
    22621 cgacatggcc cgtagggctg aagaggaaaa tgtcggcatc tatgccttta accgcgcata
    22681 cagcgaaatg attcaggcgg gtaaatttat tgagcatatg caaaaagcgc tgaaagtcat
    22741 cgatgaagaa gtcggcggag atctgagcgc ccgcgacgat tcggaaatca tcacgggccc
    22801 ggcgcacggc aggccggtga aactgtttac actgaacttt tgagcggggt tgcagcgctc
    22861 cgattgaatg ctatgctttc agaattcacc acgtgaaata atgcttgaaa atcaattaag
    22921 tatcacttat aaataatgaa aataaatgag attgcgatag tgcgttaaca ccatgcaaaa
    22981 aacgattgat tactggattg aagaaagcgc cgaagctctt caagttgcgg gacatctctt
    23041 tgagaagaag gattattcct atgcgctgtt tttcggtcat cttgccgtcg aaaaaatgct
    23101 caaggcgatt tatgtagcta agaccgggga gcatgctccg caaattcaca atttagtaag
    23161 actggcggag aaatccggac tgtcgttgca cgccgcacgg aaggatgtgt taaatagaat
    23221 aaccgccttt aatctggaag caagatatcc cgacgaaaaa cggagcttcc gtcttagatg
    23281 tacggagcaa ttcacatcaa ccgaattgtc gaacatcaag gagatattac agtggctcaa
    23341 gtcgatcatg aaatcataaa tacagttcaa ttgtttattc gacgattgaa ggaagacggg
    23401 attaccgttg aatccgccta tttgttcggt tctcacgccg ttaatcgcgc gggccggtgg
    23461 agcgacattg atgtggccgt cgtatcaccg gacttttctt cggatcgttt cgatgagcgg
    23521 ctccggttga tgaagctttc cacagagatt gataccagaa ttgaacccgt accctatcat
    23581 ataaatgatt ttcaggacac cgatccgctg gtcgatgaaa taaaaaaatg tggtatcagg
    23641 ctgaccgcat aagccgctgg gagaacaatg ggacattttg atcttattaa cttcttcacc
    23701 taaaatggct taaaccatta tcaattataa catttcggcc gtcgaagatt ttcttttaaa
    23761 ccattatttt gtgaatcgtt tctgcttgcc tgtgtctatt gatgcacttg ccagttgaat
    23821 ccaccctgtg ttcccgataa tgatcagctg gtattcaata ttccaatcca tggcaaatga
    23881 cgtctgaatc ttgttttttc gagccggtat gctatgctgc gggccatgca gccactaatc
    23941 attccccgca aggaacaccg agtctcccgc aaaaagctac ccccgaatgt tcttcggacg
    24001 ctttaccggc tccgcgacaa cggcttcact gcttatttga tgggcgggtg tgttcgcgac
    24061 ctgctcctgg aacgcacacc caaagacttc gatatcgcaa cagacgccag acccggtcag
    24121 atcaaacgtc tttttcgaaa ttgccgtctt gtcggccgcc gtttcagact ggcgcatctg
    24181 cacttcgcag acggaatcct cgaagtatcc acgtttcggg ctgatgccct ctccgatgaa
    24241 tcggacatgg aaaggccggt tcccaatcat cacaaacccc gtcatctcaa ggatgattcc
    24301 ggaatggtgc tgcgtgacaa cgtctttggc acacccgaac aggatgccct ccgccgcgac
    24361 tttacgatca atgcccttgc ctataatatc gcagattttt cggtcatcga ctacacgacg
    24421 ggactcgccg atctgcgcga cgggctcatt cgccccatcg gcgatccgta cgtacgattt
    24481 acggaagacc cggtgcgcat gctccgcgcc gttcgttttg ccgcatccca tgattttgtc
    24541 atcgaacctg ccgcgtggga gaccctctgt accctttcct ccaccatttc ccgcgcgtca
    24601 ccggcaaggc tctatgaaga gatgttgaaa ctcttcctgc tcggcgccgc agggcgggct
    24661 ttcggtctgc tgaacataag cgggctcctt gccgccctct ttcccatgct cagcaactgg
    24721 cttggcggaa acacccagcg tctgaacatc atgaacgcaa atcttgaaac cctggactgc
    24781 cgatgccgga tgggaatgcc gccgtccccg gcgctcttct ttgccgcatt cttcggtccc
    24841 ctcctcgcgg aaacaacgct tgccggccgc cgggacggca tccccggccg attgacacgg
    24901 gatgccgcgt gtacggatct gatgaaagag tgctccgaga ccgtgagcat tcccggaaag
    24961 gtcgcaaacc agctgcgcaa catccttgcc atgcagcact cgcttcacag cgtgccgccg
    25021 cgccgtcctt ccgtcatcgt caagagaccc gaatttgcgg atgccctggc ctacctgcac
    25081 ctcgaaggaa cgttgggagg ggaaaatcaa tccgcactgg agtggtggga tgcgtttctt
    25141 tcagggaagt cttcttccat cattgcggaa acgccggccg atgaagcgtc ggcgaaacgg
    25201 cgcagaaggc ggaaacgcag acatcgggca aaagggacac ccgtaaagta gcaggtttct
    25261 cacggcggaa tcagagcctt tgtggccttg gctgaaccgg ttatgatccg gcatttaccg
    25321 gcttcgggat tttttaccgg aaaaatttgg catacaccgg ttcttgcgcc caggaacgga
    25381 gggcattcca gtcgcggtaa tctccctcgg gggttccctt ggactgcaaa atctttttgt
    25441 agatccagga caggttgccg taatgcatcg cgccggcaaa tgttccaagg ccgacaggtt
    25501 ttatttccgg cacttcctgc aggacggggt ccatataggc cagcacttcg gcccgtttct
    25561 gatcagtcgg tctggcaagc gtcatgcaca cgaggaaata ggcgaccggc acccgccgca
    25621 aagcttcttt gttctttttg acgaaatcca ctgcttccgg catccacttg cccatgtaga
    25681 tggcgctccc gacgaccacg ccctgatagg acccgatgtt gcgggcattc ctgatcaaag
    25741 tcacgtcggc gcttacgcct ttggcgcaaa gctctttcgc gacggcatcg gcgactcccc
    25801 cggttgaacc gtacttgctg gcgtacgcga ccaatacttt cttttccatt ttgcctgtgc
    25861 atttcccttc cggaaatttt atttccgctg cgtggctgag cccggccccc ggcatattcc
    25921 caagcatgag ggccgtgaca aagccgctgg acaagatcag catttcccgt cgactgactt
    25981 ccaatccttc cttctgcctg ttttccacaa gagcctccct ttctgctttt ttaccggatt
    26041 gtttcactgc catatactcc cgatgcgttc agaatggaag cccctggccg gaggatcaac
    26101 catagatgcc gggggtatcc ggcattatat tttttctgag agttctgaac ctgaacctgt
    26161 ttcgtggatt caacaacggc ggcatcgcgc tgggggcggc ctcgggaacc tgaagctgcc
    26221 ggagcgtatg aagggaagcc tgtgcgttcg gcagccgatg ctgttttaag aagaaataag
    26281 ccatgctgac gaacagatcc cgggggaaga cgccgcggat cttctcaaat cccaggctat
    26341 gaaatccgtc ccgaatgatc gtggcgcagc ttcgggtcag gatgtgaaag tcccggtact
    26401 tgccgggctc tttcggatcg gtcggcgtgc cgcggatttt ttcaagttcg gcgaaaaaaa
    26461 atccggatag ccttcgcgtg tcgaggccgg aaatcctcag cacatgaatc gtccgcatga
    26521 aaagcctgcc gaacttgtcg tagtaaggcc tttggggatt ctccgtgttg tcgcgtccgc
    26581 tcgccggatc cggggcgaat tcgcccagcg ccggccggaa aaaatattct tccggattca
    26641 tggcctcgtt ctcgttcatc cagtgcgaaa agttgaagac tttcccgtct gccaaaacgg
    26701 ccagatgccc cagaaacgac aaggggtatt tcacgagccg ctcattgaag tagatctcga
    26761 cgaagggcgg cgtgagcgcg ggctcgtcgg gccacaggat gaggaggggc cttcccgtat
    26821 cataatcttt ctgcggcagc ctgcgggcgc ataccttttt tccgctccag tgcgtcaaat
    26881 acccgctcca gattttttcg tcttccagat gccggctgcc gttgtctccg gccaggaaag
    26941 accgttcagc ggttttggtc agcacgcccc tgtagatgat gttttccatg gcgggaagct
    27001 cccttgacgg cctttccggc gggtttggat cagagaaaaa attttccggg attgagaagg
    27061 ttttccgggt cgagcagtcc tttcagccgc ttcatgagaa tcagacttgc cccgtgctcc
    27121 ctctccatga agcggctttt gccgatgccg acgccgtgct cgccggtgca ggtgccttcg
    27181 aactccagcg ctttcatgac gatgctgcgg ctggcttctt ccacgcgggc ccagcgcttg
    27241 ggatcctgcg gattgtccat gacatggaca tgaatgttgc cgtccccggc atggccgaag
    27301 aggtaggccg tcaggtcccg cacttcctgc ttgctgaatt ccaccatctc gccgtagcgg
    27361 gacaggggca ccgccacgtc catgatcagc ggggaaaggc cgatgtggca gcgcttgacg
    27421 gattcgtagg tctgatggcg gatttcccag aggcgggccc tttcctccgc gccgatcccg
    27481 cggtcgagcg aaagcgcgcc tttggcaaag cagatctctt ccacaaagct catttcttcg
    27541 ctgagcccct gttcgctgta tccggaaaac tccatcagca gcgtgggatt ttcctccagc
    27601 gtcagtcccc ggtcggcgtt gagcgcgtgc acgacattgg cgtccagaaa ttccatggcg
    27661 acgggcgaca ggcccgcgcg catgatcgcg tacaccgtgt cggtcgcccg gcggacggcc
    27721 gggaaggttg cccggacggc catgaagttc accggcagac cggcaagctt cagcgtggct
    27781 tccgtgatga tgcccagcgt tccttccgat ccggtaaaga gagcggccag attgtagccc
    27841 gacgaacttt tgcgcgcgcg gttgcccgcg tgaatgacat cgcctcccgg caaaacgacg
    27901 gtcaggcgca tgacgtaatc ctttgttgct ccgtagcgca ccgaccggat gccgctggcg
    27961 ttgttgccga tcatgccgcc gatggtggcc gccgcgcccg gatcgggcgg aaagaagaga
    28021 ccttcgcggg cgagctgccg gttgagctcc ttgtaaacga cgccgggctg aacatccgcc
    28081 tgaaagtcct ccgcgagcag cgccaggacg cggttcattt cgctgaaatc catgaccatg
    28141 ccgccgcgga cgggaatggg atttccctcc aggctggttc ccgcgcccca ggctgtcacg
    28201 gggactttct tccggcaggc ccagcggacc agacggctga cttcttcggt ggagcggggc
    28261 cacacgacaa cctccggaag ggaaggcgag tgaaaggatt cgtcacggga atgaagatgg
    28321 cgcacggatt gacccgcgga gacgcggcgt tcgtccagaa tggctttcag ttcggagaga
    28381 tcagcctctg tggtcatacg gattttccgg atactttctt ggcctcgccc tcggcttctt
    28441 cttcaaaccg gccggttttg gccgtctgac gctgaaattc ctcgatcgag ccgccgtagc
    28501 gcgtgatgaa ggtttcgacg aggaagtcaa agggcagatc gtcaaaggag ccgcggtcat
    28561 ggatgtattc catgtgccgc cgtccctcca tgtcaaaggg atgaagcacg gcgtcggatt
    28621 gtccgtccca gtcgagcggc ttgactttga atttttcaca tagcgacagg ttgaaggtcg
    28681 gcgtcacttt cagccagcgg ccgtccagcc aaacgtcgtt gtagccgtga ttgtaaaaaa
    28741 tgtccgtctt catcatttct ttgaggcgct ttgtggtgag gtgattgcgg acatcggcga
    28801 gatggattcg gcaggcaatg ccctgcgacc gcatcagcgc ggccagcaga accgctttgg
    28861 tcacgcagta tccgtatccc tggcgaagga tggtggttgc ccggaacgct tcgcgccggg
    28921 gatcgatccg gtaggggtcg taatagatgc cgtcgcggac ggcgtagaag agcttgacgg
    28981 ctttttcccg gggcgtcgca gcgcccgcgg cggctttttt agcgaaagcg gcaattgcgg
    29041 gggcgtcccg ctcgatgaat tccgtatgcg ccaaacagga ttccggcata gttcaccttt
    29101 cttgaggcat aatcatgacc ttgacggcgg cggggtcctg aagctgggcg cggaaagctt
    29161 ccgccgcctg atccagccca aagcggtgcg agacaatgtt ctcggtgctg acttttttgg
    29221 ttttcagcag ttcgatgacc gccgggaaat tgccgcccag gcagcccacc agcgtcaggt
    29281 tttttccgat gacggacagg ggctgccagt ggagcgcctc ttcgtaaatg ccgaccagca
    29341 gaattttgcc gccgccgcgc gccatggcga cggcctgatc gaaagtggtc tgatttccag
    29401 cgcattcgag gacggtattg acgcccagtt gatccgtcgc ctgcatgacg gcctgcagcg
    29461 tgtcttcggc agccgcgtcg atgaccggat gtgcgccgaa gcgctttgcc gcggccagac
    29521 gcgacggcct tcgtccggcg gccagaacct tttctacgcc cagagccttg aggatctgaa
    29581 cggcgtagag tccgatgacg cccaggccga tgaccgccac gatgtccgat ggttttggct
    29641 ggccgcggtt gacggaaaag caggaaatgg aaagaggctc gaccgatgcc ccgtcttcat
    29701 acgagagctc ttcgggtagc gggaaaacgc tgcggcccgg gatcgcgaac ggcacgtgaa
    29761 catattcggc catcgcgccg ggaaactggt atcccagaag cgccatgtcc gagcagcggt
    29821 gcatctttcc ctgtttgcac cagaagcatt tgccgcaagg agaaaagccg actgccgtgc
    29881 agcgcgtgcc ggcggcgatt ccctgaacgt tcttgccgac ttctacaata tcgccgctga
    29941 attcatggcc gaagatggcg ccttcatggc cattctcttt gtaccagtgc aggtcggaac
    30001 cgcatacgcc gcaggcgttg actttgacaa tgacgccgtg gggagcgcag acgggatcgg
    30061 gaacggtttg gattcgaata tccctcacgc cgtgcaggac tgctgctttc atgttgccct
    30121 ccggagattt gagttttgaa agtatatttc aaacaatggc ttccgtgtca agccgttaga
    30181 aaatgaggga ccatataaat agttgatggt gaatggtgaa tggtgaatgg tggatggcaa
    30241 agaagcaagc aatgatcgat gagtgacgag tcagcaggga acggtcgcga ccgttccctg
    30301 cgagcttctt taccgcgctt tgtttcaggc gcggcaaatc cggttgcggc cggtctgctt
    30361 ggcctggtac atggcctgat cggcgcggga gataagccct tcgggatttt cgtcggtcag
    30421 caattcagct gcgccgatgc tgatggtggc gtgaatctcc ctgctcagtc cgggctgaaa
    30481 ggatattttt tcaaaaccgg cccgaatgcg ctcggcgact tccaacgcgg cccgaatgtc
    30541 ggtgttgtgg agaatgacca cgaactcctc tccgccgtaa cggcaggcga tatcttcttc
    30601 ccgcagggtc tgaaccagca gccggcccag ggccgccagg accttgtcgc cttccgggtg
    30661 cccgtaggta tcgttgaaat ccttgaaatg atcgacatcc agcatcaaca gggacagtcg
    30721 cgtgccgctg cgccgcgccg ccgccgtttt tttcaccaag gccggattca aaaaacgccg
    30781 gttgaaaagc cccgtcagtt catccgtcac gctgatcgcc tcgaggtctc gttcccgctc
    30841 acgcagctgt tcttcttcct cctgcatgat gcggatgcgg tagcccagcg caaacgacag
    30901 gaggatggct tccgcggcgc tgccgaaaat gacgatgtgc atggtgaagg tgttgttggg
    30961 gatcaggccg taaaatttga aaaagaagac aaaagcggcc agcagaaaga cgccgaaggc
    31021 gatgaggtaa aacagggccg gcctaaagcc ggagcgcaaa gacgaaaggg cggccgtgaa
    31081 aagcacaacg gtgaccacct gtcccgaaag gtaggacaga agattgctga gccataattg
    31141 tctcaacaaa accatcaaaa tgatcgaccc catgaaaacc gccaggccct tgagcagttt
    31201 gtcgtggcgg ggagccgttt tcggtgtgtt gagaaagaca atgccgaaag aaacggcaaa
    31261 aatcatcatc agggctgcga acaccgggat gccggacatc agaaatgcgc ccagcggcgg
    31321 ccagatatac tggaaaagtc cgacgagaac gcactgccac agcagcaaaa agccgatata
    31381 ggtgatgtag aagagatatt gacggtcgcg gataaacaga tacagggcca gattgtagag
    31441 aatcatggcg atcaggatgc cggcgaaaaa tccgacgatc agaaagagga caaagctcat
    31501 tttgcgaaaa gcgtcgatgg tataaagcgt cgcacgaaac tgcagcgcat agggggtggc
    31561 gacccggacg accaccggac gggacgcatc caggttgtcg ggcagaacaa atgtcgggta
    31621 gaggaagggg aattcccggc ttttctctcc ctgccgccat cctccatgga attcaatgag
    31681 gtctggcttg ccgtccagga tgaccggaac atagagcgtg acggcgccca gggcggcgtt
    31741 gtcgaaaacc agaaaggagc ggcggctggc cagcgccgtt tcatccccgg aatcggcggg
    31801 agcgatgcgg catttaaacc acagtgttgc tcttttaaag ctgaactgaa aggaatattt
    31861 cgtgtggcgt tgaaagggtt gtccgctgct gaggatgtcc tgatactgca agcgcccgga
    31921 gtaatcgagc agatattcgg actgcgaagc gatttcgacg gcgggcacgg cgccgccgac
    31981 ccggatgggc ggcgcttccc cggcctgtgc ggtgatcgcc cacaggagga cgaggcaaag
    32041 aatgaatgga ccgattcgat gaagtgttcg cgggtgcagg atcattttca ttcctccttt
    32101 gatgcatccc gaaacgcggt tttttcagat tccggagtca aacatggacc acattttgtc
    32161 gcggtcgatg tccgggcggt tgtcccggag cctgacgatt ccctgtccgg aaagctcctg
    32221 aaaaatgttt ctgatttggg acggctcttt tcgggtcagc cgggaaatct gctcaatgtg
    32281 ctcatcgacg gtggctttgg gctgatcgag aatttgcagg aggatgagca tctgcgttct
    32341 ccgatgcgtg aattcttcca gggccgcgtt ggaatttttc aggcgtgagg cgaattcgcg
    32401 cagcatggcc agcgcgacct cccggctttc acgaatcagg ttctggaagg tccggtcgtc
    32461 aatcacggcc aggtggctgt tttcgagcgc tcgcgccgtt gcggtccttc ggacgtcaat
    32521 gagcgccgcc atttcaccga agtactgccc cggccccatt tccgccagcg tttttttaac
    32581 atggccgtcg gaacgctcca ggcggatgcg ccctgaaagc acgtaaaaca tctcgcagcc
    32641 ggaatcattt tcccggaaaa ccacttcgtc ccgtttgtaa acgcggccga atttctggaa
    32701 aaggcgcgca tccaccttca ggagagaatc atgaggttta agcagggctt tttccaaaag
    32761 cagcatgtcg cgccgctcga aggaagacat gatttccgcg caaaacagaa aaatcagcgc
    32821 cacgtaaaaa acccagatga ccaggatgac cacggtttcg agggagccga agatgacatt
    32881 atagcgggtg tagttggcca ggtaccaggt aaaaagctgc ttggcgattt ccaccagaaa
    32941 cgcaaaaatc gcgctgccgg ccagaaccac ggaaaaacgg atttttgccg tgggaatgat
    33001 ccggtacacg aaggtcacca ggagaacgac catcagaaag ggaatgagat aacgcagagc
    33061 gatttgcgcc ggcattgtca gggaaaacag aacaccgccg gcccagccgg agggctcctt
    33121 tgcgagcagc gccgccagat aggtaatgac gacgcttaaa ccgccgatca gccaggcggc
    33181 cggaatcatg gcgacggcca tcagtttgga gacaaaataa tttcgtttgc gccgggaccg
    33241 gaaaatgatg ttcagcgccg attcgatggc gttgaagatc gcggccgaca gccagacgag
    33301 gcccaaaagt ccgatccagc ccagcacctt gctcttgccc tcgatttgcc ccacctgctg
    33361 aaggagcgct tcggaaaaat agggatgaac cccctgaacg gcgccgatga tgtcagtctg
    33421 aagagtggtg ttggaactga agaaggatcc ggcggcgatg agggttagaa taaaaagcgg
    33481 aatggcggac aggatggcat agagcgcgat cgccgccgcc tgattggcgt cgccgttcag
    33541 gctgaagttg ttgaccgcgt cgaaggcgat atcccacgcg ccggcggcct tcatctgcat
    33601 cgattctttg cgcgttttgc gccgcgcccg ggagtcttct tttttttccg tcataaatgg
    33661 attcgactga cactatcgtg tttttgaggc gcatcttaca acggaataag catccggtca
    33721 aaatgattat ttaaaagaag cttgcgtcaa acgaccgcca cggaagcaaa acgcggatta
    33781 cttcgaggaa cccgtcaggc ggcagtcgtt caggaacgac tccacttcat ggaaaacttc
    33841 ccgtgcggcg gggccgagga caatgccgtg ccggttgaaa tcaatccagg cgaaacgctt
    33901 ctgatgcgcg cccaggcgct gaaagatggc gggcccgctt tccggagcaa ccttggggtc
    33961 gtgccggccc tggaggatca gcgccggaat ttgaatgtca ggcagcctcc gccggacgct
    34021 ccgcatcagc tttttgacct gaacaaaaga gctgaccgga cagaggagat aattgatgtg
    34081 gggattgtcc gcgtcgtttt tgacgaattc caccgcaagg cggttcaggc cgacatgaga
    34141 gcagcaaaga ttaaagccat gcagaagctc ggcaaacctt gatgaaaaac tcttgaaccg
    34201 gagcggcgcg ctgacggcaa tgaccgcttc aaaatcatgg ggtttcccga tggcggattg
    34261 cagcgcgagg ccggcgccgg tggagaaacc ggccaccagg attctacggc agcaggtttt
    34321 gagaatggcg tggccgcgat caaccgaatt cagccagtcc gaataatcgc gaaccaagag
    34381 gtcttttgcg gatgttccgt ggccggcaag ccggggcgcg taaaccgtca tacccctggc
    34441 gtgcaaaaat tccgcccact cgcggacttc cgccggcgcc gccatcagtc cgtgaatgag
    34501 cagtacgccg gtgtcggacc cgggcgcgcg cagaaaaaaa ggcattccga tatccggagg
    34561 tttgctctcg cccgccttat aatttttttc gtaatcctga agaaataatt tcagttcctc
    34621 cgccaggagc agatccgttt gacgtccggg ttgcgcggga gaaggaaccg tgctcatggt
    34681 taaacgggac gggaataaac gccgtagatc atggcgtaat gcaaaacgcc gccgatcagg
    34741 accatgatgt gaaaaagttc atgaaaactg aaaacacccg gaatcattct tggctttttg
    34801 acggcataga taacggcgcc cagtgtaaag gcaatgccgc ctgaaacgag gaggacggcc
    34861 tgggaagagg acatattgga cagcatttgc ttgatcgtga aaagcgcgga ccagcccatg
    34921 gacagataaa taatcgcgta gatttttctg ggcgcccccg gaaaaaaaat ctgcgaaatg
    34981 acgccgacac ccaccagccc ccactggaag gctatcatcc accatctgta gggatcctcc
    35041 agacaaaaat aacagatggg tgtaaaggtt ccggcaatca tgaaaaaaat ggccagccgg
    35101 tccatttttc tccagaagga cagttcattt tcctcttttt tgaaggcatg gtagagcgtg
    35161 ctcgcggaaa aaagaaaaac aacggatatt ccgtaaatca gcgcggtgac caggccggag
    35221 gccgaatcac tggcggccat ggccagatag acggtcccga cgacggcggc caccgctccg
    35281 gcaaagtgtg aataaaaatt aaacagctcc tgcgttttga ctttcatgat cctctgccct
    35341 cgatggtttc tttgtcttca cgaaaagtct gttcacagat tttctgaaaa agcgaggatt
    35401 aattgcggcg gatgaaccgg cggccggggg aggcgcgaaa gagtaaatga tattgcaaag
    35461 acttgcttcc tgggctatgt attgcgccat gcgcaggata tcgaatatga aaacgaacgc
    35521 ctctttctcc cgttttgaca ggggcgttga acttcattcc ctggaccgct tcggcctcag
    35581 aatcaaagat gccaggcctt ctgccgccag gatttttcat ttcgtactgg tccttttgct
    35641 gctggcagcc gccgcgtgcg accggacgcc gcagacggga agcgccggtt ccaaagggga
    35701 caagccgccc gcctacggcg atatcctggt acggggcgat atcggggatg ccagcaacct
    35761 gattccgctt ctggcttccg actcggcgtc gcacgccgtg ggcggcatgg ttttcaacgg
    35821 tttggtcaag tatgataaaa atatgaatat cgtcggtgat ctggccgaat cctgggagat
    35881 ttcgccggat ggtctgtcga tcaccttcca cctgcgaaaa ggcgtccggt ggcacgacgg
    35941 ccggcccttt accgccgccg acgttctgta cacctatcag gtgacggtgg atcccaaaac
    36001 acccaccgcg tatgccgggg attttcagaa agtcaaaaag gctctggttc tggacgacca
    36061 cacctttcgc gtcacctacg acaagccttt cgcgcccgct ttgatcagtt ggtcttctgc
    36121 ggtgatgccc cggcacctgc tggcgggaaa agacattgcg caaagccctc tttcgcgcca
    36181 ccccgtcggg accggtccct accgcttcaa ggaatgggtg ccgggtcaga aaatcgttct
    36241 ggcgtccaac gaggattatt tcgaggggcg cccctgtctg gacggccgga ttacgcggat
    36301 cattcctgat accgccacca tgtttctgga gcttcgcgcg cagaatatcg gcatgatggg
    36361 gctcacgccg ctgcagtaca cgcggcagac ggaaaacaac ctgtttcggc agaatttcaa
    36421 caaataccgc tatctggcgt ttgcctacac gtacctgggc tacaatttga aaaacccgct
    36481 ttttgcggac cggcgcgtgc ggcaggccat atcccacgcc atcaataaag acgaaatcat
    36541 cagcggagtg ctgctgggac tgggcaagcc ggcgacgggg ccttacaagc cgggcacctg
    36601 ggcatgcaac gacaaagtga agatctacca ctatgacccg gccaaagcgc gcgaactcct
    36661 gaagcaggcc ggatgggcgg acgcgaacag cgacggggta ctcgacagag acggccggtc
    36721 ctttgaattt gaaattctga ccaatcaggg caatgagacc cgccagaaat gcgcggagat
    36781 cattcagcgg cagcttgcag aagtcggcat tgctgcgaaa atccgcgttc tggaatggtc
    36841 ggcgtttgtc actcatttca tcaacaagcg gcgctttgac gcggtgattc ttggctggac
    36901 cattccgccg gacccggacg cctatgacgt gtggcactcc ggcaaaaccg cgccggaaga
    36961 gctcaacttt gtttcctatg ggaatcccga agccgacgag atgctggaga aaggcagaag
    37021 caccttcaac cgggccgaac ggaagaaata ctacgaccgt tttcaggaaa tcctggccga
    37081 ggatcagcct tacacttttt tgtatgttcc cgacgattta atcatcattt cgaaccgcgt
    37141 tcgcggcata gagccggcgc ctgccggcat cggccacaac ttcatccggt ggtacgttcc
    37201 caaagacgaa cagaaataca cgatgacgag ataagctggc atattcgagc ctttagcaga
    37261 gaacggtctt gccgcccgca agggtgatgc aaaccgtttt ctacggttaa aaaaacactt
    37321 gacaatagaa cgtgcgttct atataagacg ggccatgaaa aaagtccgcg acataaaatc
    37381 cgtcgaacag agcctggtgt cgttttaccg ccggcagaag cgcatgccca cgtacgccga
    37441 gatgatggat ctctttgagg tgcggtccaa gagcgtcgtt tcttactgga tcgagaagct
    37501 gatcgaaaaa ggcattctgg ataaagacgc gcagggattt ctcaagctgt cgggcgtttc
    37561 cttcggcatt cccctggtcg gcaatgtggc cgccgggatt cccgcgtcgg cggaggaaat
    37621 ccctcgggat gttctgtcca tggacgagta tctcgtgacc agacccgaat cttcttttct
    37681 gctcagagtc acgggagact cgatgatcgg cgcgggcatt atggagggag acctggtcgt
    37741 ggtcgagcgg gagcgaacgc cccggaacgg cgacatcgtt ctggccgaag tcgacggcca
    37801 gtggaccatg aaatatttcc gccggcgggg cggaaaagtt gttctggagg ccgccaaccc
    37861 ggcttatgct gcgatttgtc ccaaagaaga actcaaaata ggcggcgtga tcaccgcctc
    37921 cgtcagaaaa tatctgatgt aacccggatc aaaaaaatgg agcaatcagg atgcgtgcga
    37981 tcatcagtgt ttccggatgg ccctgcggcg ggaacggcca tcgaagcgtc gcgttgagtc
    38041 ggaaatcgtt tgcggcggca atgcgcaaaa caccgaaaac aggccgcggc ccgaccatgg
    38101 cggaaatcga ccggctgatt tccgcggcgg gcgccgtggc gttgatcctg tccgcctttt
    38161 atttcgcgcc gattgtcgga tcaattttta tgaaatagct gcgcagcaaa gggtgaagac
    38221 cgccttttgc cgtcttaccg cttcagctca tgccggatcg cgaggccgat ggtttccatg
    38281 acgtagggtt tttttacgta ggcgcccgcg cccagccgct gcgccgtcag cacccgttcg
    38341 gtttcggaaa atccgctgac cagaatcgtt ttctgccgcg ggtcgatgtc gcggattttc
    38401 cggtaggttt ccagaccgtc cggccccgga gacatgatca tatccagcag aaccagatcc
    38461 acctccttct cctgaaggaa ttttaccgcc tcttctccgc cggaagccgt ctcgacctca
    38521 tagcccagtt tcatcagcag actggaagcg atttcccgct gctcggcaat gtcatccaca
    38581 accagaatcc gttccccgcc gccccggtag tcctccagcg gcttcctttg ctgcggcagc
    38641 gccgcctcct ctctggttac ggggaagtag aggatgaagg ttgttccttt cccctccttc
    38701 gtttgaaggt caatataccc gttctggtcc tttacggttc cccagacaat cggcaggccg
    38761 agccccgttc cgctccatcc catcgtcttt ttggtgaaaa acggctcaaa gatcttctcc
    38821 cgatgctctg ccggaattcc catgcccgta tctgaaacga tcagcaggac gtaatccccc
    38881 tcctgaactt cttcatggct gatcaggatt ctgtccacgt aacggttttc cgtgcggata
    38941 acaatctcgc ccgcctcggc aatggcctcc gccgcgttgg acaccaggtt gatcagggct
    39001 ttttccagat gaacggctga gcctttgatg ttcagaagac gggggtcgca ctccgcccgg
    39061 aaggttatcc gcggatggtc gttttttatt ttttcaaaaa agggggtttt aagaaaaccg
    39121 tcaacgatcc gattgaggtt cacgatgccc gacacggcaa ctccccgccg ggccagcgtg
    39181 agcagatcct gtatgatcac cgctcccttt tccgtggact ggaggatctt ctccacgttt
    39241 tttctggctt tgtgatcttc gggaagctcc agcagaagca gttccgaata gccggacaga
    39301 acccccaaaa cattgttcag atcatgcgcc acgcctccgg ccagctttcc cagagcctcc
    39361 atcttttccg cccggtgcag ccgctcctcc agcttttgct tttcctcttc cgcttttttg
    39421 cgtttggtga catccctcga aacgccgtga agtccggtca ggactccctg atcgtcgcga
    39481 agcccgctga tgatggtctc catccagcgg gtggagccgt ccttgtgata atattccagg
    39541 agcagcgtcg ccagccgggt cggatccccc agggcctgtt tttccagggc gagctccgcc
    39601 gccatggctt ccagtccgcg ggagagagac tccggcgtga tttgctgttc gaccgcctgg
    39661 tgcatccgtt cttcctggct gaatcccaac acgctctgga cggagggcgt cacgtagagc
    39721 gtcttcagat tcatgtccga aatccagacg atatccgtca ttttttccgt caggagtttg
    39781 tatcgctttt cattttcccg cagcttctgc tccgccagtc tgcgctcggt gatgtcccgg
    39841 gcaaccgcaa ccagaccggc cacttttcca tcgcgggtaa tgggagaacc gctgacttcg
    39901 ccgaactttg ccgccccgtc tctggccctg aattcgtaaa tggtcgccgg aagggctgtc
    39961 ccctgcagaa ccaggccgat gtgttccagg gccgtttcca tcgaatccgc cgcgaagaga
    40021 tatccaaggt cccgaatgga ccggccgatg aattcccgcg gtttgtatcc cagcgctttc
    40081 tccacgctcg gagaaatgct gatgatcctg aggccggcgt ccacggtaaa aatgacatcg
    40141 gtgacatgct caaaactctg ccggtagatt tcttccagca tgcggagctc ccggttggcg
    40201 gcttccaggt cctgcatttt cttttcgagc ttgcggaaaa gagcctcatt gtgccgacgg
    40261 aaaaactcca tgtcttcccc aaggggtgag gatgcttcgg gcggggcggc ttttccctcg
    40321 tgcaggaact cctccaggat gccggtcagg atttccggct cctggggctt gagcagaaat
    40381 cggtccgcgc cgaggctcag ggcaaagtcc tcgtccttcg gttcggtata ggtggcggta
    40441 taaaagacga aagggatgga tttcagcttc gggtccgcct tgcattcccg gcacagggcg
    40501 tagccgtcca tgacgggcat cagaatatcc gagacgatca ggtccggcgg ctgaacccgg
    40561 atggcgtcca gggcgtcttt gccgtttccc gcctcacgga cgctccatcc ccggctcttc
    40621 aaaatgctgg ccagcagata aaggttgttg gggttgtcat cgacgatgaa aatctgtttt
    40681 gccatggtgc gttccccggg acgtcggccg tcttgaaaaa aaagtgccgg atgctaccgc
    40741 ttttccgctc cgatttccag aattttatcc acgttgaaaa ttcttttttc ccgccgctcc
    40801 aggcagatcg cttcgaggcc caggtaggga tagcccttgt actccatttt gcccacgaac
    40861 aggggcctca ccgtccggcg cgatttttcg tcctgactct tgaggtaaag gatttcgatg
    40921 tctttctttt cagcaatcgc ctcttcgatc cattttattt tatcctcgcg gctgagggtt
    40981 ttggcggccc gggcgtactg gagattggtg agaaatttca ccacgcgcca gtccatcaga
    41041 atgtggcttt tttgaagagg cttcttgagg acaatgccgt cgagggtcgt gcagcgcgac
    41101 agcgccacat acatctgccc gtgggcaaaa gtgccccgcc ccacgtcgat caccacgttt
    41161 tcaaaggttt ttccctgact tttgtgaatg gtgacggcaa aggccaggcg caccgggtac
    41221 tgccggaacg atccgacctc ctccgagcga agctcttcat ctttgatgaa aaaccggtag
    41281 attttccagg tatagggcga gatgcgcgcc gtatcgccgt tgtccagttc agccacgatc
    41341 acgtcgtctc cttcgtcgtc tttttcgaat ttgcggattt ttccgatggt gccgttgatc
    41401 cactggccga aagagtcgtt gttgagcatc atgatctgcg cgtttttttt cagtttcaaa
    41461 tccaccgcgg tcggcagata ttcctttccg aaatcgcctt cgatttgtcc tttggctttc
    41521 cagatttttc ccggcagttc ggccagccgc ttttcattga tcgtgtcggc caggtcgttg
    41581 gtgctggtga gggccaggta aaaggatcct gaaggcgctt cgaaaaacgg gtcgcagcga
    41641 gaattgaatt tggccagatc gtcatccgtc acggagcggt tgcggatggt gttgagcaga
    41701 cggacaaact cgtcgtcctt ctgccggtaa actttttcca gctcgacata ctcgatgtcc
    41761 agcgcctcaa agacttttgc gctgaaaaaa tagggcgtcg ggtaatggtt tttgaaaatc
    41821 tcgcgctccg ccgatgagac gaccggcggc aactgataca gatcgccgat aaaaatcatc
    41881 tgcacgccgc caaaaggctt tttttcgctg gggccgttga agcgcagaaa tttatcgacg
    41941 cagtccagca gatcggcgcg caccatcgaa atttcgtcaa tgacgatggt ggtcagcttt
    42001 tgatagagcg taagcctgtc cttttccgcc tttttcctgc gcttgatgga cgccggcgtc
    42061 acattcggct tgaaatgaaa aaaggaatgg atcgtctggc cttcgacatt gaccgccgcc
    42121 acgccggtcg gcgccagaat caccgccttt tttgacgtgg tgtcgcggaa atacttaagc
    42181 agggttgact ttcccgtgcc cgcccggccg gtgatcagga tatgcttctg cgtatcctcc
    42241 atgagcgaaa tcgcccgctg gaattcggaa ttgaaatcaa tctcgaccgg actggatggc
    42301 tggttcatga cacgttcctt tatggcgttg atgcttctaa acacaatttg gtccggtatt
    42361 caatcaggat tatttgcttt ttccgggccg ctggtataaa aaacagccgg ttggcaaaat
    42421 caggcgcaag gaaggcgttt taggctttga aacgaaaatc atctcatcag ggaggaaata
    42481 tgcgcaaggt ctggttgacg gttttgggag ggttgctgag tgtggttttt tgcatgtcgg
    42541 ttttggcgca gacgccgccg ctttcggacg gcgctcaaga ctcgctgatc aagagccagg
    42601 tggaaacggc ggtcagcctg ctggcggcga ttcacgcccg gcagcagaaa ggggagatga
    42661 gcgcggataa agcaaaggaa ctggccgccg gcctgctgcg ggaactgcgc tatggaaagg
    42721 atggatattt ctgggccgac acgacggagg gcgtcaacgt ggtgctttac ggccgcaagg
    42781 atacggaagg gagaaaccgc ctgaacgacc gggacgccaa aggcgttttc tacgtgaagg
    42841 aatttctggc caaaggcaaa accggcggct atgtcgaata ccggtttacc aagcagggcc
    42901 aggccgcgtc tctgcccaag cggtcctatg ttctgctctt taaacccttc ggctgggtgg
    42961 tcgggacggg atattaccgg taaaacgcac aggtgatccg caaaaaaaag tcagggccgc
    43021 aacgccctga cttttttgtt ttaccgggtt ctgtcgggaa tccgaatgaa tgcaacggtc
    43081 gtattaccag acttccggga aagccatgtt ggtgtacaaa tcctcgagtt ttttcttgtg
    43141 gccgcgttcc atcgacgcaa gctcctggaa aacctttttc tgcgccttgt ccgcgcttgc
    43201 tttggccaat tgcgtgtaca tctgcatggc ggcaagttcg ttcttgatgg cgatgaccaa
    43261 accgtcaacc ggtttcagat cggccgtcaa cggaggcgtg ggaagctcgt ccgccacctt
    43321 gtaatccgcc gattcggtaa aagtaaattt ttcagccccc ctggccgcat aaccttttaa
    43381 cgtgagcctg tgctgtgctt cttcgtcggc cagttctttg aaaagctttt tgagatttgc
    43441 gtccttcgct tttttggcta cgccgctgta aaatttattg gctgctactt cggctttaat
    43501 ggcgttggca atgatctttt tgaattcact ctgggtcatg gtttcactct ccttaacgtt
    43561 ttgtaggtgc tctgtctgcc ttatttttca agttggttat tcgaagtttc gtgaatccgc
    43621 ttaaaaatac gccggtcacg gctatgtcct ctagcatagc tgatttttgt tgtcaaacga
    43681 ctggcgagca aaattcggct tgacattcat tttttgttta tgttagtgga aaaccgcagg
    43741 acgaaagagg atggtagatg tgatgacaaa cattttaaac agaaactggt ggtggcaggc
    43801 gatgaatgtc tgcccgtaag ctcctgaaaa tcaccgagca tgggcaggga ggctccttcc
    43861 cgtgactcgt cgatatatga agaatgccat gggaagaaaa gccccatggc attttttttg
    43921 gccccggtgc ggccgggcgc aaaaagagtc tgctgaaaaa agcaagggtc attctggcaa
    43981 aggagagaaa aaatatggaa caggtcaaaa cattattgga agatcaagaa ataccaagac
    44041 aatggtacaa catcatggcg gatttgccca cacccatgaa accgccgctg catcccggca
    44101 ccggccagcc ggtcacaccc gacgatctgg ccgccatttt ccccatgaat attattgaac
    44161 atgaagtctc cacgcagcgc tggattgata ttcccgatga ggtgctgcaa aagtacctgc
    44221 tgtggcgtcc atctcctctc tgccgtgcgc gcaatttcga aaaactgctg ggctgtccgg
    44281 taaagattta ttataaaaat gaaggtgtca gccccgccgg ttcgcataaa cccaattccg
    44341 ctcttccgat ggcctattac aacaaggtgg ccggaaccaa gcgaatcacc acggaaaccg
    44401 gcgccggcca atggggcagc gcgttggcca tggcctgcca gatgtttggc ctggagtgcc
    44461 gtgtctatat ggtaaaaatc agctatggac aaaaacccta tcggcgcctg atgatgaaca
    44521 cctggggggc cgaatgcatt cccagcccca gcactttgac gcaatccggc agagatttcc
    44581 tggccaagta ccccgattcc cccggctcgc tgggtgttgc catcagcgaa gctatcgagg
    44641 atgcagtcac cagcaaagta aagtcccact atgccctggg cagcgtactt aatcatgtgt
    44701 tgatttatca gtcgattatc ggtctggaag ctcaaaagca aatgcagaag ctcggcatct
    44761 atcccgatgt cgtcatgggt tgttgcggcg gcggcagtaa ttttgccggt atcgcagcgc
    44821 cgtatgtctg cgataaaatc catggcaagc aggtgaaaat tgtcggagcg gagccgtcct
    44881 cctgtcccag catgaccaaa ggcccgttcg cgtatgactt cggcgatctg gcgcagaaaa
    44941 cgccgctttt acccatgtat acgctggggc acgattatgt ccccgcaccg attcacgcag
    45001 gcggtttgcg ctaccacggc atggccccga tcgtcagcca tctggttcgc gaaaaattta
    45061 tcgaaccccg cgcctatgac cagttaaaaa catttgacgc cggcgtcaag tgggcgcgct
    45121 cggagggctt cattcccgcg ccggaaacca accacgtatt ggccgccgtt gtcgatgaag
    45181 caatgcgcgc caaagaagaa ggcaaggaaa aagtgatcct ctttaattgg agcggccacg
    45241 gcctggtgga tatggcctct tatgacgcct atctttccgg aaaactggag gcctatgagc
    45301 tgccggatga ggaaatccag cgagccttgc tggccattaa agatttcccg aaaccataag
    45361 gacttcattt tgcgtgaacg gtcgcggccg ttccctgcga aggccgtcag ggaaataaaa
    45421 tttcttgacg gcttttcttt ttttcgccaa ggtggcaggc aatgaaaaca ccgcatgatc
    45481 ataaaatcac caggtgcccc aaactgggcg acgaaatgac gtttgcctac tgcctgcggg
    45541 aagcggggaa tctgccctgc gcgcggattc ttttctgctg gcaggccgcg tttgacgcgg
    45601 cttcgctgct ccaggaaaac ctgacgcagg aggagctaaa gcgcttcgcg gaaatcctgc
    45661 ccaaagacaa ggttgtcagc ttgattgaaa ttctcgaaaa aacaaagagg caaacatgaa
    45721 gaacctggat ttattcagcc agggaactcc ggcggacgcg ctggagacgg cgccgctggc
    45781 cgaaagaatg aggccgctta cgctcgccga ttacatcggc cagccgcacc tggtcggcga
    45841 aggaaccttg cttcggcgat ccatcgcgga ggacaagctt ttctcgatga ttttctgggg
    45901 gccgccgggt tccggcaaaa ccacgctcgc gcggattctg gccaatgaaa ccaaagccaa
    45961 ctttgtcagt ttttccgccg tcctttccgg cgtcaaggaa atccggcagg tgattgacga
    46021 cgcccgggat ctctgggagg ccaaaaagca gaaaaccatt ttgtttgtcg atgaaatcca
    46081 ccgcttcaac aaggcccagc aggacgcttt tttgccgcac gtggaaagcg ggctcgtcac
    46141 gctgatcggc gcgaccacgg agaacccttc tttcgaagtc atcgcgccgc ttctgtccag
    46201 aacccgcgtg atgctgctca aacagtttgc ggaagaagac ctgacgggca ttctgaagcg
    46261 cgcgctttcc gatccgcaaa ggggtctggg ccaatttgcc attcaaatcg acgacgaagc
    46321 gattaagttt atcgtcggga tttccgacgg cgatgcccgg acggcgctca acaacctgga
    46381 ggcggcggcc tcgatggtcc agtcgctgcc tgaggaagaa aggatgatct cggcgaaatc
    46441 cgccgaagaa gcgctgctga agaagtctct cctgtacgac aaggacggag aggaacatta
    46501 caatctgatc tccgcgcttc acaaaagcat gcgcggaagc gatccggacg caacgctcta
    46561 ctggctgggc cgcatgctgg ccgccggaga ggatccgctt tacattctgc ggcgcatggt
    46621 gcgctttgcc tccgaagatg tcggcaacgc cgatccccat gccctggtgc tcacgatggc
    46681 cgcccagcag gcgtttcatt tcatcggcct gccggaagga gaactggcca tcgcgcaggc
    46741 ggccgtctat ctggccgtgg cgcccaaaag taattccctg tacaccggct acggaaaaac
    46801 aaaggattta attcataaaa ccggctatct tcccgttccc ctgcatatcc gcaacgcacc
    46861 gacgaaactc atgaaggaac tggagtacgg caaggactac aaatacgcgc acgactatca
    46921 ggatgccttc gttccccagg agtattttcc ggaaaagctt cagggaaaag tgctttactc
    46981 cccgaccgac gccgggtatg aaaaaatcat cagggaaaga gtcgaagagt ggcggcgccg
    47041 gaaaaacaaa gcaaaaaagg aatcggagaa aaaaggataa aaaaacggga ggctggttgg
    47101 aagcctcccg tcaggagaga agagtgaagg cctggttacc ttcccgggtt atttttgctg
    47161 tcggctaatc atgcccgtga tattttagtc gacacttgtt ctttttctga tgaaggataa
    47221 agcagttccc gtgccaaaat tgttttaatg tttgcaagta attgatatat aatgcatatt
    47281 ggtcaaacaa ccgtttcatt ttcttccctt ccaatgccag gcctttgaca acaaatgtcc
    47341 actggacgat cagtaaaaaa attaatcaaa taacattagg aagatagatg agagacaatt
    47401 tttgtccacc tttggacaaa aatgtccacc cgggcagtat tccgtattga cttttttctt
    47461 gaaaagcaca atgatgacga aaaaaataca aattagattt caaaagcgtc cttcataata
    47521 cacgcaaaac aaggacaata tgagaatttt tcaaaaaaat gaaaatatcg tccagcgtgg
    47581 aacccgtccg tataacggga gctttctcca gccgctggct gtcggcatcg tgtcggcagt
    47641 cctggtagcc ctgattctgg tgatgggcta cctggatatt cggcgaaacg agaccaatct
    47701 ggtcggattt atggaagatc aggcgctgac caccatcagc gttcttttgc gccagacgga
    47761 agacaatctg aaaagcatcg ccgccgccgg ggggaaaatg ccttcccacc tcaaaacggc
    47821 caaagaggcg gaatccgcct attccattca attggtttac gatgccatca ctaagctggg
    47881 gcaaaatatt gacgagcagt ggaaaaagaa attgctgaac aacgcatacc ttcgcaggtt
    47941 cgcggaggaa aatcatttct ggtatgtggc cgtcctcaat atgcgcggcg acatgattta
    48001 tcaaagcggg cccctcaaaa ccgacatgct ggccaaaggc gatttgatcg tcaacgggcg
    48061 caaggcgacg accattgaat tgctggaaaa aatccgcatc cggcaggaca tcggctacgc
    48121 cggggtgaaa ctcaagagaa gcggcggaat cgccattttc tgtctggatc ggaaaagcag
    48181 gctgtactgg agcctgaaag tcgccgtcga aagggccatg agcaaaatgg gcgagggcca
    48241 cggcatcgcc tacctgcagg tcttcaatga ccagaaaacc ctgctgggga atataggtga
    48301 tctgccggcc ggcctgaccg tttcggacct caacgttcag gaaatgatga aagggaaaaa
    48361 aagaatcctg agccggaaag tggaccgtca gggaatcaag attctggatg tagccgcccc
    48421 cttcctcatg ggaaatccct acctgccgaa agaaaagatc gccgggatcg tccgcatcgg
    48481 tctggaccgc ggttccatgg ataaaatcat tgccg
//
LOCUS       DAOPAF010000005        45755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_942_length_45755_cov_55.602013, whole genome shotgun sequence.
ACCESSION   DAOPAF010000005 DAOPAF010000000
VERSION     DAOPAF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 45755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45755
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_45755_cov_55.602013"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLQ77_01100"
     CDS             complement(<1..730)
                     /locus_tag="PLQ77_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPL09310.1"
                     /translation="MDNIIGGKLVADALVERGVDYVFSLSGGHITPIYQFLENSPVTI
                     FDVRHEQAAVFMAEAWARINRKPAVAMVTAGPGFTNALTGIASTRLSNAPVILISGCV
                     GLDSVEKLDLQDMTQLPVIEPMVKKALVCYMPERIPEYIDMAFRAAMSGRPGPVYLEF
                     PCNILNAKVDMAAVKKPGTTLSSKPVDRENTRKVVDLLKAAKNPIVIGGSGLWYSDAE
                     KEILEFVEKIGIPIYTAGAGRGVVP"
     gene            complement(793..1632)
                     /locus_tag="PLQ77_01105"
     CDS             complement(793..1632)
                     /locus_tag="PLQ77_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL09311.1"
                     /translation="MIYNDLKGKTALVTGAGKKTGIGYAIAEKLASCGCNVIIADLGA
                     PPAPESPLQPVVSEDMKAIASELAKAYAVRTLAVAVDVADNQAIGAMVETIKKSFDHI
                     DILCNNAGASFGVPNTVLGYDEAAWMKTIDVNLFSVFRMSRAVLPLMAGKPASIINTA
                     SRAGKVPPIFNSAYAVAKAGVIMLTKVMAKELGGGGIRVNAICPGQIATDLEKWRFGL
                     EASFFGTTIEEREKEMCKTIPLGYIGLPRDAGDLVAFLASDASRYITGQAVNLDGGQC
                     MEL"
     gene            complement(1622..3385)
                     /gene="xsc"
                     /locus_tag="PLQ77_01110"
     CDS             complement(1622..3385)
                     /gene="xsc"
                     /locus_tag="PLQ77_01110"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HPL09312.1"
                     /translation="MKKVKMTPSEAIVETLLAEGVDHVCGIVGSAFMDMLDLFPTAGI
                     KFIPVRHEQSAAHMEDAYCRISGRCGVVTGQNGPGITNMVTSVAAANMGHTPLVIISP
                     SAGTPTIGWDGFQECDQMSVFKSVTKAVVRVPHPKRAADCLRTAFRIAYADRGAVLYD
                     IPRDYFYGELEEKILKPSQYRVDARGCGSMEQLNRAAELLSKAKNPVIVSGRGVVDAD
                     ALDIVKAIAEHLTAPVAVTYLHNDAFYGNHPLSVGPIGYMGSKAAMKTLAKADVMLAI
                     GTRLSVFGTLPQYDINYFPENAKIIQVDINPKHIARTHPIEVGIIGDAKEASAEILKR
                     LKAMDGRRKPDEKRLAAVARERKKWEKEIVDLAMVDGNPMNPRRVLLEMSRALPDDAI
                     VTTDIGNVSSTANSYLKFNHPRRHIAALTFGNTGFAYPAALGAQIARPKAPVIAIIGD
                     GAWGMSLHEVSTAVEHNLPVIACVFRNMWWGAEAKNQVDFYNNRFIGVDIPNPESFVP
                     VAKAMGAHAMQVSRSQDIREAFQTCLKSRKPTVLEFLVDGKQLAPPFRKDALALPTRF
                     LSKYAHLDHRRWVDEGGSDDL"
     gene            complement(3437..4630)
                     /gene="nrfD"
                     /locus_tag="PLQ77_01115"
     CDS             complement(3437..4630)
                     /gene="nrfD"
                     /locus_tag="PLQ77_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPL09313.1"
                     /translation="MLEKALHGSLRYRIWIACLILVGCAGFFFYLRQLDYGLGITGMS
                     RDVSWGLYIAQFTFLVGVAASAVMLVLPYYLHDYKAFGKITVLGELLAVAAVVMCILF
                     VFVDLGRPARVMNVLLHPQPSSILFWDMIVLSGYLLINLVTGWVILACTKKEIPPPGW
                     IKPVIYLSIPWAISIHTVTAFIYAGLPGRSFWLTAIMAPRFLASAFASGPALLILVCL
                     ILRRWGRFDAGEKAIQALAKIVAYALAVSIFFVGVEMFTVFYSQIPEHMHHFQYLFMG
                     LEGHHNLVAWMWIFAVLSIAAFVMLILPGVRRNEVWLALACIAVFVSLWIEKGLGLVI
                     TGFIPSPLGAITEYHPTGPEITITLGVWAAGFFLLTVFYKIFVHVRGGTDGSPCGPAV
                     DKMIH"
     gene            complement(4638..5420)
                     /locus_tag="PLQ77_01120"
     CDS             complement(4638..5420)
                     /locus_tag="PLQ77_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL09314.1"
                     /translation="MTSSRRDFLKIAGASALTMMTPAIFVPAWAKDVIVIPPGQGLAL
                     KKALAAGRWAMTVDLTKCTADCTDCIAACHAIHNVPDFGNPKDEIKWIWKESFASVFP
                     GEQNAYSGEKLQKLRPPVFCNNCENPPCVRVCPTGATFQRPDGIVMMDYHRCIGCRLC
                     MAACPYGARSMNYRDPRPCIAKINPDFPTRSKGVVEKCNFCEERLAKGTLPACVEACR
                     QKALTFGDAENPRSEIRRLLKTRFSLQRRVHLGTGPQIYYLL"
     gene            complement(5417..5818)
                     /gene="dsrJ"
                     /locus_tag="PLQ77_01125"
     CDS             complement(5417..5818)
                     /gene="dsrJ"
                     /locus_tag="PLQ77_01125"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HPL09315.1"
                     /translation="MKQHKWGLILLGLAIFAIAVTFPFWYGKGKTAPPVLSLETPEIA
                     GLADKKCVEDAVFMRANHMKMLQAWRDEAVREGKRVYTANDGRTFEKSLTGSCLQCHS
                     NKEHFCDRCHHYVGAAPSCFDCHIVPAEVKK"
     assembly_gap    5967..6066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6160..7755)
                     /locus_tag="PLQ77_01130"
     CDS             complement(6160..7755)
                     /locus_tag="PLQ77_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPL09316.1"
                     /translation="MSLAKIPKPEELMKIGYKPPATAWMKTPVDFRPGTWSYAAAAKN
                     LAALDMPNPRAWQPSDSDWKLPENWRQIILDGMKERLEKYRSFRLFMDICVRCGACAD
                     KCHFYIGSGDPKNMPVLRAELIRSVYRRYFTKAGMCFGTLAGARDLTVDVLKEWFYYF
                     YQCTECRRCSVFCPYGIDQAEITMIGRELLNLIGCSINWVLEPAANCFRTGNHLGIQP
                     GGIKDMLEFLADDIEDRTGVRVDVPINQKGADILFVTPSGDYFADPGTYTCMGYMMLF
                     HEIGLNYTFSTYASEGGNFGLFTSHEMVKRLNAKIYAEAKRLGVKWILGGECGHMWRV
                     IHQYMDTMNGPADFLEEPVSPITGTRFENARSTKMVHIVEFTADLIKNGKLKLDPSRN
                     DHIRLTFHDSCNPARAMGLFEEPRYIIQKSCNHFYEMAEDTIREKTFCCGGGAGLGND
                     ENMEMRMRGGMPRAMAVRDVVRKHGVNRLGCICAIDRVTLPPLMDYWVPGVTVMGVHE
                     LLANALVMKGEKERTTDLRGDSI"
     gene            complement(7758..8741)
                     /gene="dsrM"
                     /locus_tag="PLQ77_01135"
     CDS             complement(7758..8741)
                     /gene="dsrM"
                     /locus_tag="PLQ77_01135"
                     /inference="COORDINATES: protein motif:HMM:NF038037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HPL09317.1"
                     /translation="MNILLSFIAILAIALAAAAAGRADAPGWFRILPYAAFGLFLAGF
                     VYRVIRWAKAPVPFRIPATCGQGKSLPWIKSNPLDNPAGPLGAIGRMAAEIFLFRSLF
                     RNSQTEIVDGRPVYGSAKWLWFFGLMFHGSLLVVVLRHLRFFTEPVLPAVTGLSALDG
                     FLEIGVPALYLSDATLLAGLAFLFLRRAVLPQVRYISLFADYFALLLIAAVAATGLLM
                     RHFFPVDLNAVKTMAMGWATFAPVAPKDGSVILYMHLCFVFILIAWLPFSKLMHLGGI
                     FMSPTRNMINNNRSLRHINPWNTPVKVHTYKEYEDEFRDKMKAAGLPVEKE"
     gene            complement(8769..10379)
                     /locus_tag="PLQ77_01140"
     CDS             complement(8769..10379)
                     /locus_tag="PLQ77_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPL09318.1"
                     /translation="MKDRITPADISRGSAGLCRIEPGDLAPLPPPYEDFNEAVLKPLA
                     AEKIKGLETSPDDTLAIGIAEPANKQEEEELVRRFLAGLKKLFEKENNWTFLQPLLLS
                     MEHCARCQTCSEACPVFEMSGRQEIYRPIFRSEVLRRIYKKYLKPGGRITAKWTGDID
                     LNWKAIARLAELSYRCSLCRRCASVCPIGVDNGLIAHEIRKLFSQEMGIAPKELHEKG
                     TVLHLRAGSSTGMNPLAAKDSVEFIQEEMEEITGCKVEIPWDVQGADILLLHNAGEML
                     AWPENVGAFAILFQAAGLSWTLSSEAAGYDAVNYGLWYDDVQFARVAVKHADIARRLK
                     VKKIVLGECGHAHKALTVVADRLLTGDLNIPRESALVTLEDIVMSGKLTFNPQKNDFP
                     VTLHDPCNVVRSMGIVAPQRRILGKIAPRFREMTPHGVNNYCCGGGSGFAIMSGYNFA
                     EWRNLVSSRKKFLQILQAFESEPAETPKYVCAPCSNCKGAIRDMIAYYHAEERSHLYY
                     GGLVELMVNALEGMKKPIIHFGEEPSWK"
     gene            complement(10376..11311)
                     /locus_tag="PLQ77_01145"
     CDS             complement(10376..11311)
                     /locus_tag="PLQ77_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPL09319.1"
                     /translation="MGFYIFSYLCTVIFLVAVACRVYRQLTLPVHVRWEIYPVQHEPA
                     NKAAYGGSYMEELNWWKKKRERSPVNELLYMAPEIGLMRGLWKENRNLWLFSFPFHAG
                     MYLMLVVFALLLLSAVLFLLGLAEDSAVSSIINVLIAAAGWIGILAGTAGSLGMLYKR
                     LADRNLRAYATPADYFNLVFILLFFVCALLSALAVDPCFPAARLYVLGLLTAGAAAPA
                     YVPGQSLFGALTILLGSLLAAYIPLTHLSHMFMKFFLYHKVKWDDSPNTPGSPIASAI
                     RRNLQYRPTWKAAHVDADGKKTWQDIASAGPKEMK"
     gene            complement(11454..11774)
                     /locus_tag="PLQ77_01150"
     CDS             complement(11454..11774)
                     /locus_tag="PLQ77_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPL09320.1"
                     /translation="MPQIELAGKIYEVDEDGFLQELDKWNEEFAQAYAHTEDIEGPLT
                     EEHWKIINYLRDYFAKFGIAPMVRKVCKDNGIDMKKLYELFPTGPAKGACKLAGLSKP
                     TGCV"
     gene            complement(11777..13210)
                     /locus_tag="PLQ77_01155"
     CDS             complement(11777..13210)
                     /locus_tag="PLQ77_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HPL09321.1"
                     /translation="MNQQRPRFIMAALRGGAGKTTLSLAVTVALRRQGLMVTPFKKGP
                     DYIDAAWLGRAASGPCYNLDTYLMGARQVVRSFSRHAAEGTFSLIEGNRGLFDGVNAA
                     GEFSTAELAKLLYAPVILIVDCDKVTRTAAAMVLGCLHLDPAVDIRGVILSRVNGKRH
                     ASVIRRAVEESTGLPVYGTVPRLPDLPFVQRHLGLIPPAEHADVQQALDTAGAIARDY
                     LDIPSLVKIARSAPPWKVGAFPKAAPSVRDMDPPVTIGVISDAAFQFYYAENIEALAD
                     RGARVVRISALDDEELPPVDALYIGGGFPETHAGRLAANEPFRLCLKQAAEAGLPIYA
                     ECGGFLYLGESLTVGGKRYPMTGALPVAFGMEAKPQGHGYTTLAADAENPFFPVGTTI
                     NGHEFRYGRILSRRQEETHTAFRVLKGTGLDGQREGLCRKNILAGFTHVHALGTPEWA
                     KAMIAKAFQYREEKRNEECTINIMRRF"
     gene            complement(13237..13461)
                     /locus_tag="PLQ77_01160"
     CDS             complement(13237..13461)
                     /locus_tag="PLQ77_01160"
                     /inference="COORDINATES: protein motif:HMM:NF020263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HPL09322.1"
                     /translation="MSDDLKAKITELFREKSKGDKKMFYIRDVTKWLPGEDRHAIQNT
                     VKELLDEEVLKYWSSGSSTYLILTEFFPKE"
     gene            complement(13464..14528)
                     /gene="dsrB"
                     /locus_tag="PLQ77_01165"
     CDS             complement(13464..14528)
                     /gene="dsrB"
                     /locus_tag="PLQ77_01165"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HPL09323.1"
                     /translation="MAARTDIGPPNYKTMLPPVIERNYGDWLYHEILKPGVLMHVSKS
                     GETLYSVRAGSPRLISTDHIREICDIAEKYCDGYLRFTSRHNIEFLLDDKDRVEPLIA
                     ELKDRGYPVGGTGHSISNIVHTQGWVHCHTPATDASGPVKALMDEIYDHFVEAKLPAH
                     VRIALACCLNMCGAVHCSDIAILGIHRTVPKTDNSRVPNVCEIPSTVASCPTGAIRGD
                     MKNKTVTVNEEKCMYCGNCYTVCPAMPIADPDNDGISIWVGGKVSNARSAPMFSKLAI
                     PFLPNNPPRWPEVVNAVKNIIEVYASHARKHERVGEWIERVGWERFFSLTGIPFTDQH
                     IDDFDLATETFRTSAQFKWR"
     gene            complement(14530..15717)
                     /gene="dsrA"
                     /locus_tag="PLQ77_01170"
     CDS             complement(14530..15717)
                     /gene="dsrA"
                     /locus_tag="PLQ77_01170"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HPL09324.1"
                     /translation="MANSETPLLDELEKGKWPSFVKDLKQAAGKNPMARDLIHQLELS
                     YKDRIGHWKHGGIVGVKGYGSGVIGRYSDRPDLFPNVQAFHTMRVNHPTGWFYKTDTL
                     RKLCDIWDKHGSGLTNMHGSTGDAILLGTTTDHLQSCYDDLSNAGFDLGGSGSALRTP
                     SACVGPARCEWACIDSLDLCYNLTMTYQDQMHRPMFPYKFKIKISACANDCVAAIARS
                     DLSVIGTWKDDIQVDQKEVLAYASSINIQKEVCDLCPTRCMEWNGKELKIYDEDCTRC
                     MHCINLMPKALRPGKERGASILVGGKAPIVRGALLSWVIVPFIKLEPPYDELKNLIER
                     IQDWWDENAKSRERLGELIQRLGMRVFLKAVDLPAVPQMVKAPRTNPYVFFWPEDLKK
                     EVK"
     gene            complement(16048..16206)
                     /locus_tag="PLQ77_01175"
     CDS             complement(16048..16206)
                     /locus_tag="PLQ77_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09325.1"
                     /translation="MDDVKIKSEMEKMEYEPLLPIEKKLITWSLILGVVLLVGLVWVS
                     YTFFPAGQ"
     gene            complement(16229..17674)
                     /locus_tag="PLQ77_01180"
     CDS             complement(16229..17674)
                     /locus_tag="PLQ77_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPL09326.1"
                     /translation="MGSKQGLFKTEDWWSVWLGLFIFIISLGSLLGLDLLGWAVTPKV
                     WMEISKSIAPASKGYAELHPIISIILTFLAVLGLLMVGARAMGYDLKKFVYGFTIIFW
                     LTYCCNIIGHYAVIAAQTPADLKKFGLDWSLKLTGEAGLILALLVGLFIGNFMPKFAN
                     SLKEAARAEWFIKTAIVIMGVGLGVKSAEQLGLATSMMFRGLCAIVEAYLIYWAVVYL
                     IARKYFKFNREWAAPLASGISICGVSAAIATGGAIRARPIIPVMVSSLVVIFAVVELI
                     ILPFVATELLPNQPLVAGAWMGLAVKTDGAAVASGQIVESLIYAKNAAQGINYQPGWV
                     VGAATTVKIFIDIFIGIWAFILAVIWTTKINPTKGDKARAGEIWDRFPKFVIGYFVTF
                     IIMLIIGLNWPDIKSQIKISASETDVFRKLFFALTFFSIGLASNFKKLWEEGIGKLML
                     VYVIALFGFIIWVGLFISWIFFQGVMPPLVQ"
     gene            18195..18908
                     /locus_tag="PLQ77_01185"
     CDS             18195..18908
                     /locus_tag="PLQ77_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPL09327.1"
                     /translation="MIENLINNLALYIQGSVILAFAAAYLGGVVISFTPCTYPLIPVT
                     VGFIGAQKATTKLRGFLLSLFYVLGLSVTYAALGAVAALSGRLFGQMQTTPLVYFLMA
                     NVCLVMGLAMLDVFKISIPIPQKILNYTGGGKKGFLPSFLLGAASGFVIGPCTAPALG
                     VLLGFVALKTHVAMGIGLLFVFAFGMGTLLILVGTFAGLIAALPRSGAWMTVITRVFG
                     VILIGAAEYFLYTAGTLAF"
     gene            18920..19426
                     /locus_tag="PLQ77_01190"
     CDS             18920..19426
                     /locus_tag="PLQ77_01190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPL09328.1"
                     /translation="MQKIYRVFVALFLILGLSAIPSAGQFIPNAPFKPQYTPAPDFTL
                     KDLKGKIFRMSAQRGKPVLIFFGTTWCPGCRAEIPKYKKVYETYASRGLEVIYINIME
                     PAKKVARFAQANALPYRTLLDEDGSVGALYNAVGVPMMILVDKDGNMIKVGFSSTQMP
                     LDKVTPVQ"
     gene            19508..19723
                     /locus_tag="PLQ77_01195"
     CDS             19508..19723
                     /locus_tag="PLQ77_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPL09329.1"
                     /translation="MTTDNWFAEFAKSETDRWIGGVCGGLGEHTPIPSWVWRLLFTLL
                     VLCFGTGFLLYILLWIFVPRRAANRPG"
     gene            19776..20441
                     /locus_tag="PLQ77_01200"
     CDS             19776..20441
                     /locus_tag="PLQ77_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09330.1"
                     /translation="MKYREDIYVYEWANYFDNNCNSYYIGGNVKALVDPGLTRYLPDL
                     LNQMANDGIRKKDIQYVINTHSHPDHFQGSELFDHEKVKIALHRKEMDFLKGIGGELY
                     GLFGMTVPQMQINMQLEDGRLELGGGLFKVISIPGHTPGSIGLYWPAKKALFSGDVVF
                     QQNVGRTDFPGGNGAHLKKSIVTLSDLDVEMLFPGHMSIIEGHDKVQDNFNIIIQNIF
                     PYI"
     gene            20474..22159
                     /gene="ettA"
                     /locus_tag="PLQ77_01205"
     CDS             20474..22159
                     /gene="ettA"
                     /locus_tag="PLQ77_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPL09331.1"
                     /translation="MANEGNKVIYSMIGVSKVYEKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDAEFLGKTILSAGYTVGYLEQEPRLDEAKTVRRIVEQGVQATID
                     VVNDYNAVNEMFAQEMSDEEMAKLCDRQAALQEKMDALDAWDIDSRLEMAMDALRCPP
                     GDTPVQVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTVI
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKNEEKTESARVKTLER
                     ELEWIRMSPKGRHAKSKARIKAYEELLDRNLEKESGTRELFIAPGPRLGDLVIEADAV
                     SKGYGDKLLVDAMTFKLPPGGIVGVIGPNGAGKTTLFRMITGREMPDAGVIRIGETVK
                     LAYVDQSRDALDPEKNIWEMISDGQDIIPVGGRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALESFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDRKSRLGAAASQPHRIKYRQLTRR"
     gene            22219..22581
                     /locus_tag="PLQ77_01210"
     CDS             22219..22581
                     /locus_tag="PLQ77_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09332.1"
                     /translation="MKKISLALMAAALLISGLPPAQAQEYGKVRALQERAAYVIRQKN
                     DFVVRVLRSYKIPHEVNDQGVVVRINMNDRWMDVTAIEIVPMLREAEDRSRQVAAHEL
                     FFFTADGILDVVSALTIR"
     gene            complement(22568..22906)
                     /locus_tag="PLQ77_01215"
     CDS             complement(22568..22906)
                     /locus_tag="PLQ77_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09333.1"
                     /translation="MPVYIQIILWVVGFAVFVLAVMYFAGFGLRRVCFKIIAELEEAG
                     AFKVSRAIRLQDERKNFFRVGTGNLRPRALHLLITDKLVVKTPDGKYYLDREKLAELK
                     SGDGKSSSGS"
     gene            complement(22968..23369)
                     /locus_tag="PLQ77_01220"
     CDS             complement(22968..23369)
                     /locus_tag="PLQ77_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09334.1"
                     /translation="MLNKIVIRYTDKRLVKGVTVDFAPNRDIFHVTPAGFSPSDKPLE
                     VRVADLKAIFFVKDFSGNPKHEDRKNFEPGIPVLGRKIRVLFKDGEVLVGTTQGYQLN
                     RQGFFVIPADAQSNVERCFVVTKATREVKFI"
     gene            23613..24422
                     /gene="murI"
                     /locus_tag="PLQ77_01225"
     CDS             23613..24422
                     /gene="murI"
                     /locus_tag="PLQ77_01225"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPL09335.1"
                     /translation="MNIQANQAIGVFDSGIGGLTVVRSLMERLPFENIIYFGDTARVP
                     YGVKSVETIRRYALQIAEFLLKQDVKLLIVACNTMAAVAYQAIRDLSPVPVLEVIEAS
                     AKIAVARTRSKSIGVIGTPATINSNAYARAIHILDRDAKVFSQACPLFVPLVEEGWFE
                     HPATRLVAEEYLKPVMAERVDTLVLGCTHYPLLKPLFQDITGGQVRLIDSAEAMADIA
                     ADLIRQNNLGSTANRPPEYLFCVSDVPYRFQTIGERFLGRTLPRVEMVRLQ"
     gene            24419..25891
                     /locus_tag="PLQ77_01230"
     CDS             24419..25891
                     /locus_tag="PLQ77_01230"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPL09336.1"
                     /translation="MMKVLLAGYNVDYDLIRDLREKSGLTQDITPETISAAYARISRS
                     PKSVDQLRVDARAEVEKARRSNRNIVFEMGHSSVAEHAVFNIDVIGVSRLLVEEIEKF
                     RLCSYTEKSQRYVLFDRDFVVPEEIVRAGMEKDFVETVRMQNDFYHELYEWLRPWVFE
                     QNRELAENPANRSLLEGWAKEDARYAIALATRTQLGMTINARNLELMLRRLASLPLAE
                     ARLCSEKLYAATRDIAPSLIRYTRATDYDRLTRPRLRTKAEMLLAKRPQEAPSGKFAD
                     VRLVSATPGADRQAAAALLFASSGLSYGQCQSRSARLNHRELKGLFQTVFDNMQPYDA
                     ALRELENIDLQFELVVSAGCFAQLKRHRMATIIAQDYDPRLGVTVPPSVRAVGRQKKF
                     MTVMEATQRAHDRIRNKLPSAPPYLLTNAHRRRVLMKFNARELYHLSRLRADAHAQWD
                     IRNLSEKMLRQARRLMPLTLMMACGKDGFAALHKKAFPGK"
     gene            25915..27291
                     /locus_tag="PLQ77_01235"
     CDS             25915..27291
                     /locus_tag="PLQ77_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL09337.1"
                     /translation="MATPKKLPIVLFATTISSFTTPFMGSSVNVALPAIGRAFSMDAI
                     SLSWVASSFLLASAIMLLPMGRLADIYGRKIFFLSGSLIFALSSLLAVLPETESLFIA
                     LRVAQGFGAAMIFSTGTALLVSAYPAGERGKMLGINIAAVYTGLTAGPLIGGLLTQHL
                     GWQAIFIANAALGAGAAGLALRMEDEKTPAAAGEPFDLSGSFLYAAALFALMYGFSRL
                     PSSRGAVLIAAGGIFFIIFIRRQLQKPSPLINIRLFLRNRVFAFSNLAALIHYSATFA
                     VTFLMSLYLQHVKGLTPSQTGFLLVIEPLLQAVFSPLAGRLSDRHEPRIISSIGMSIT
                     VVGLAGLIFVARDTPLWTILFCLALLGTGFALFSSPNVNAVMSSVDGKFYGIASAMQA
                     TMRMVGQMLSMGIAMLVFAWIIGSRGIDYENGELLITSAKIIFAILAVICVGGILASL
                     ARGRVHKA"
     gene            27311..28123
                     /locus_tag="PLQ77_01240"
     CDS             27311..28123
                     /locus_tag="PLQ77_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPL09338.1"
                     /translation="MIVNGDAVYFCERGLKMRTQWISFAIGLAAGCFGGLIGIGGGII
                     MVPLLVEILHVDQCRAHGTSLVVLIFTGISGAVSYGLHGQVNWKAAVLLAVPAMLLAA
                     AGARLADRLPDWKLKKFFGGFLIFCSALLLLKPILADWIGVLPDAVSNVALVVTGGAT
                     GFLSGLMGIGGGTIMIPAMILLGGFTQHVAQGTALLVMVPSGFVGAFAHWKLGNVAGK
                     LLFSMVPGIVLGAFAGAGIAQFIPEEPLRWMFVLVAVYMGWRYVKVVSPNTC"
     gene            complement(28186..29454)
                     /locus_tag="PLQ77_01245"
     CDS             complement(28186..29454)
                     /locus_tag="PLQ77_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL09339.1"
                     /translation="MNAKRNPWLWVPSIYIAEGIPYMIVMTVSVVMYKNLGLSNTDIA
                     LYTGWLYLPWVIKPLWSPFVDLFRTKRFWIVAMQLAIGGSFACVALTLPASDFVRYTL
                     AMFWIMAFSSATHDIAADGFYMLGLTTPEQAAFVGVRTVFYRVATIASKGGLVILAGT
                     LQKCGYPAACAWSITFVVVGAFFLALCLYHLLVLPYPEADHSAPLDKGRGVVAEYFRI
                     ITLFFRRKDIVVIICFFLFFRFAEAQLVKMVAPFLLDSRAEGGLGLSTAQVGWIYGTV
                     GVVALMLGGLLGGYVIYRNGLKFWLWPMVIIMHVPDVIFVYLSHAQPDNIWLIGSAVA
                     AEQFGYGFGFTAYTMYMIMVSQGEYKTVFYAIGTGIMALGMMLPAMSSGWIQEKLGYI
                     HFYYWILLTTIPGFIVTALVKIDPEYGKKA"
     gene            complement(29646..30968)
                     /locus_tag="PLQ77_01250"
     CDS             complement(29646..30968)
                     /locus_tag="PLQ77_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPL09340.1"
                     /translation="MIDHEPNIQAALLQHTSEARLILEGDFILPDGRVIKGGYMVRAD
                     GGEVFLTDTEGRGGIRGKDILLSPAAPAESFFTVSGIKIGIDFHWQRAQKQSFRGALL
                     LSGQGQSAFHLINIVPLEEYLASVISSEMAASAPLEFLKAQAVTARSWLTAMLAKKKS
                     IRARHPKTKGEIIVWQDVNDHQGFDVCADDHCQRYQGITRIVSENVNRAIDATRGIFL
                     TSAGEICDARYYKCCGGRTDVFATAWEDASFPYLTSITDGKKQRAPIHTEKDAAEWLR
                     AKPDAYCNTNDPEILRKILPSFDLETLNFYRWRVEYQPEELEEIIRSKSGIDFGRLKN
                     IEALQRGPSGRICRLKIEGSLGAAVVGRELEIRRWLSPTHLLSSAFVVSAKRDPDGNA
                     LGFLLEGGGWGHGVGLCQIGAAVMASQGRSAEEILSHYFTGAMLKKLY"
     gene            31202..32122
                     /locus_tag="PLQ77_01255"
     CDS             31202..32122
                     /locus_tag="PLQ77_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL09341.1"
                     /translation="MAHIWPIGGGKGGSGKSFITSSLGRLSAGLGRKTLVIDLDLGAA
                     NLHTLVGVPYPSKSLSDFLQKKTERLDDTVVETPFENLFLISGAQDHFAIANLPHEQK
                     VKTMRAIMKLDYDCILLDLGAGTSFNTLDFFLISQNGIFITTPEPTSIENVYRLIRSV
                     YFRRIRYGFSVSQFKALEEEAIEQFGEGASGRPEFMMNVIARDYPERFRELQKDFDAF
                     RFRLIINQLHRRDNPALGAHVCRIIEKHMNLHVEFMGNVSYDDHVHDAICQQVSYLDR
                     YPYTKTAGELRAAGRQIFQPAGRQLMLQYV"
     gene            32163..32843
                     /locus_tag="PLQ77_01260"
     CDS             32163..32843
                     /locus_tag="PLQ77_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL09342.1"
                     /translation="MRKSFAELNYYEMLDVSPTAAAFEIRQAYGTALQIYRTGSAASH
                     SFFTEAERREILSLVEKAYRTLADELTRKAYDDELASRGEIDRPAQAGPDAKKPVGIF
                     NISRVRDRQPVPANQESLKSKIRESERISELTARSSLSGAGLKEIREELGVAIAQIAQ
                     ATKVRQDHLVNIEEDRVERLPAAIFLKGFVKSYLKYLCLEPVEELSARYMETIARLLR
                     REGAQEQE"
     gene            32857..33522
                     /locus_tag="PLQ77_01265"
     CDS             32857..33522
                     /locus_tag="PLQ77_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09343.1"
                     /translation="MESDTIQTPEGDLVIRFVGHSSLIFFWAGKNIHADPVSLEADYA
                     RLPKADVILITHGHGDHMDVNAVNIIKIPATKIVGNDEVGSQIPGTLVMKNGEERVVE
                     GLKIEAVPAYNLKHMRAPGKPFHPKGVGNGYVVTLGGLRVYIAGDTENIPEMRRLQNI
                     DIAFLPMNLPYTMTPEMAADAAQMFHPKVLYPYHQGETDTAKLLALMKDHKDIDVRIR
                     TMK"
     gene            33594..34424
                     /gene="dapF"
                     /locus_tag="PLQ77_01270"
     CDS             33594..34424
                     /gene="dapF"
                     /locus_tag="PLQ77_01270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPL09344.1"
                     /translation="MNGNGVAFWKASGSGNDFIIIDNRDLSLNVGELPAFARSVCRRK
                     VSVGADGLLLVEPSSAADFRWRFFNADGSAAAMCGNAARCVARFAFENGIAGQTLSFE
                     TQAGIIRAEVAGDVAKVRLTDPSSLKQDIRIHLSGGECGLDCIDTGVPHAVCFVASAE
                     ACDVTATGREIRRHEVFQPEGANANFALVINRHKMRVRTYERGVEDETLACGTGVVAS
                     VLAAAGRRFVDSPVDVIVQSGETLRVYFTRRDGAFSEIYLEGKVKIVYQGTLFEDAYR
                     "
     gene            34442..35617
                     /locus_tag="PLQ77_01275"
     CDS             34442..35617
                     /locus_tag="PLQ77_01275"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPL09345.1"
                     /translation="MFIVQTAERLKKIPPYLFMELRKKINQAKADGVDVISLAIGDPV
                     EATPGSIIDELCRTARDPLNHRYPTDEEKGMLALRREIARWYARRYGVELNPEKEILG
                     LIGSKEGCHHFVLARVNPGDIVLMTDPGYPAYRASILMGEGVPCNVPILPQNDYLPAL
                     EDIPSETAKKASAMFLNYPNNPTGACATQEFLNKLVAFAREYDIAVCYDNPYGEIVFA
                     GQERISFLQAPGAKDVGVELNSFSKPFNMCGWRIGMACGNPDLIAAISKVKENTDSGI
                     FNAVQFAAIQALTGEAGFIEEMLSIYGRRRELVLKTLQKIGIDFQPPRGTFYLWVPAP
                     KGMTSLEFTNRLFDKTAVVVAAGTAYGRYGEGFIRISLTVPDDRLNEAMARIEKEFS"
     gene            35617..36648
                     /locus_tag="PLQ77_01280"
     CDS             35617..36648
                     /locus_tag="PLQ77_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPL09346.1"
                     /translation="MEMGILGLPQSGKSTLFEIMTGVKISPSHTETVVRGQASVPDER
                     FDKLVEIYKPAKVSPARVPFVDVHAVGEHPWEAIRQNLSGTDGILHVVDGFSLPDISI
                     ALDACRKLEDELVLSDLIIIEKKLERLAKMSKKAIAPQEAAQNELLPRLKDCLEAGKP
                     LRRAGLTAQEVFTLKSFSFWTIKPELVVVNVAEDNPAFADEFAKAAHLAEPVMGICCK
                     IEAELAGLDAADQKEFLASMGIAEPAFGRIIRAAFSLLGRMSFFTVGEDEVKAWVIPH
                     STKAPKAAGAIHNDFERGFIKAEVMSFDDFMAHGGSQAQVKSAGRLRLEGKEYVVADG
                     DIITFRFNV"
     gene            complement(36638..37057)
                     /locus_tag="PLQ77_01285"
     CDS             complement(36638..37057)
                     /locus_tag="PLQ77_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09347.1"
                     /translation="MKSKKRNSLIFLFLAVLLLSSCSTAQTSAAPSSPLRPVILTGTH
                     WQATYLAFETAKKTFPGDKCEKDDEEGVIRIHRDSFFNGNTLITIRFQRINDNNWFVN
                     VSSEGAGLNQPLVNRSVAETEYYLKALEDAAAAPVRR"
     gene            complement(37410..38306)
                     /locus_tag="PLQ77_01290"
     CDS             complement(37410..38306)
                     /locus_tag="PLQ77_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09348.1"
                     /translation="MKKGWRGIALLLAMAVCGPLLFAGISTAAGPESEEKIFQSGAIL
                     TVAESKRLIGKAVAQMPIVKNALANGMVIIIKGTTNAYVAEEITGKKADHAAFVTGRI
                     EPEKGTKNLPEAKAVNHVILEKGKIVDIPLGEAAKKLKPGDVVIKGANALDYRNKMAA
                     VNILHPEGGTTGITMPMIVARKVHLVIPVGLEKVVAGDVAELTLKTREPVQLLPPASG
                     KSTNRFPGHLIPSQWLITGEIVTELEAIKILTGVTAFQCSAGGVSGAEGGVWLVFRGT
                     RDQVKKAMELANSVQGEPPYSE"
     gene            complement(38459..38553)
                     /locus_tag="PLQ77_01295"
     tRNA            complement(38459..38553)
                     /locus_tag="PLQ77_01295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38517..38519),aa:Ser,seq:gct)
     gene            complement(38620..38705)
                     /locus_tag="PLQ77_01300"
     tRNA            complement(38620..38705)
                     /locus_tag="PLQ77_01300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38669..38671),aa:Ser,seq:tga)
     gene            complement(38739..40013)
                     /gene="serS"
                     /locus_tag="PLQ77_01305"
     CDS             complement(38739..40013)
                     /gene="serS"
                     /locus_tag="PLQ77_01305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPL09349.1"
                     /translation="MLDIKYLRQNLEAVRRKMNERGQNIDFDRFIGLDAKRRDILQAV
                     ETLRNERNSVSRQVGELKKKKEDASALIAKMGDVSAKIKEYDETLRVTEEELNAFVMI
                     VPNVQHESVPQGGSSEDNAVVRTWGEKPSFCFAPKQHFELGESLNILDFGRGAKITGA
                     RFTLYRGAGAQLERAITNFMLDLHTANHGYTEVFTPFMVNAESMTGTGQLPKFKEDLF
                     KIEGMDYYLIPTAEVPVTNIYRDEILEEDKLPVYLVAYSACFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFSKPEASYDELEKLTANAEEVLKRLGIPYRTVCLCTADLGFSSSKTY
                     DVEAWMPGQDTYREISSCSNFEDFQARRAAIRFRRKDSGKVEFVHTLNGSGLAVGRTV
                     VAVLENYQQADGSVIIPEALRPYMRGLERITP"
     gene            complement(40107..41699)
                     /gene="murJ"
                     /locus_tag="PLQ77_01310"
     CDS             complement(40107..41699)
                     /gene="murJ"
                     /locus_tag="PLQ77_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPL09350.1"
                     /translation="MDDPTEKSENAKVAKAAGIVGAATMVSRVLGVVRDMVIAAFFGA
                     SWMTDAFWVAFRIPNMLRRLLGEGSLTISFVPVFTEYLQKKTKPEALELADNAFTILS
                     VILAAVSALGIALSPVIVGLIAPGFMANPDQYQLAVFLNRLMFPYIFFIALVALCMGI
                     LNSFRHFTSPALSPVMLNIAMITAAVALHDKFDQPITALAAGVLAGGVLQLAMQWPAL
                     GKFGVRFHFRFNLRHPGIRQIGALMLPAILGAGVGTINVFVGTVLASLLPSGSVTYLF
                     YADRIMEFPLGIFAIAIGTAALPSFSAHAAAGKTEELKSSISFSLRLLLFLTIPAMAA
                     LMALHLPIISVLFQRGAFDAQAAVYTGQALFCYALGLWAYSVLRVFVSAFYSLKDSKW
                     PLKAGIITLVVNVVFSLTLMYPLRHNGIALAGSISAAVNVGVLAFVLRNKIGPYLDRA
                     FYTSVFKIIASSAAMLAAIGLVEYGMPWNTSSGFRERLIYLVLAVLTGAVAYFACAYL
                     LKSPEMRAFLKMIQKRLTGSGN"
     gene            complement(41732..42049)
                     /locus_tag="PLQ77_01315"
     CDS             complement(41732..42049)
                     /locus_tag="PLQ77_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPL09351.1"
                     /translation="MTDTLLIRETKNGLSFDIHVNPHASRAAIAGISGGMLKVKVTAP
                     PVEGAANDACIELLAKKLGLRKSLLKISAGAKGRKKTILVSDINRTELEQRFRSLDFP
                     PDA"
     gene            complement(42063..42359)
                     /locus_tag="PLQ77_01320"
     CDS             complement(42063..42359)
                     /locus_tag="PLQ77_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPL09352.1"
                     /translation="MFALSNFIIALAKVIDIVLTVYLWIIVARALISWVNPDPYNKIV
                     IFLYRVTEPVLGPIRRMLPRHSLPIDFSPLIVLLIIIFLQYFLVQTLIQAARSF"
     gene            complement(42488..43279)
                     /locus_tag="PLQ77_01325"
     CDS             complement(42488..43279)
                     /locus_tag="PLQ77_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL09353.1"
                     /translation="MLKHFKVGDNNIACWINPADFGAHAQGLVFLHGSGGNSGAWSEQ
                     YSVLHKSFNIAAVDLPGHGQSEGSGEKDILAYVLRLKEILEVLKLKRPVLVGHSLGAA
                     IALGFASQHPQDASAVVAAGGGLTMPVNPDILDGFAKQPDLILDLMCKFSIAKENRPK
                     LFGPLRESLGQTSVDVVAGDMLACSKFDLTGELEKIRVPVLAVCGKEDKMTPPASSER
                     IAAGIAGAKLVLIEGAGHMAMLERPEAFNDALITFCREIVSDPRP"
     gene            43398..44213
                     /gene="proC"
                     /locus_tag="PLQ77_01330"
     CDS             43398..44213
                     /gene="proC"
                     /locus_tag="PLQ77_01330"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPL09354.1"
                     /translation="MFTGKKVAILGGGKMGGILARGMITRKLLAPQNITVTDIDPQRL
                     EELRSALKLRVSDDNRQAVKGADIIVLAVKPQNFPDLLKEIRQAANSSKLFISIAAGV
                     TTDAIEKWLTKKPRVLRVMPNVAALAGEGAAAVAKGHFSRPDDVQYALAMLGAVGLAV
                     EVEEKLMDAVTGLSGSGPAYCFVMIEALADAGVQLGLGRDLAEKLAAQTMLGSARLCL
                     QGSHHPAQLKNMVTSPGGTTAAGLRVLEEGKIRATLTGAVEAAAKKAKELAGS"
     gene            44236..45024
                     /locus_tag="PLQ77_01335"
     CDS             44236..45024
                     /locus_tag="PLQ77_01335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL09355.1"
                     /translation="MEIRGKTIIVTGAASGIGRGLCRRFAKEGAKAIVAADINEAGAK
                     AVAGEAGGEAFACDVRKEADIIRLVKFTEDRWGGVDLFCSNAGIIAIGGYEVDNETWQ
                     RIWEINVLAHVFVARAVIPGMIRRGGGAIMITASAAGLLSQIGSLPYSVTKHAAVGMA
                     ENLAITYGGQGIQVFALCPQAVESEMTRGHEGGGVAGVDGLMKPEQLADAVMESFAAD
                     EFLILPHKEVKTYIQRKAADYNRWIQGMRRLQDRFLTGAPLKKV"
     gene            45071..45418
                     /locus_tag="PLQ77_01340"
     CDS             45071..45418
                     /locus_tag="PLQ77_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPL09356.1"
                     /translation="MDVERCASVDNLQDLDQALLADDRVIAMVYATWCPFCRKVLPVF
                     EKWALKEKRRLLLVADDDERAADVYDIDIFPTLILFEKGRIVKRLDGRPGVGLSEKQI
                     ADFIKSCPPAEPG"
     gene            45438..45746
                     /locus_tag="PLQ77_01345"
     CDS             45438..45746
                     /locus_tag="PLQ77_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09357.1"
                     /translation="MDIFVRYCGGCNCQLDRSKLVSDVQRGLGPEFHLTTDLSGAPCP
                     AGLLVCGCPAACVWNSEVSAAARRWVIVAGKTVNAVEITEDRLAEAVAVEIKSILFSE
                     "
BASE COUNT         9833 a        12911 c        13018 g         9893 t
ORIGIN      
        1 ccggcaccac gccgcggccc gcgcccgccg tgtaaatcgg aatgccgatt ttttcgacga
       61 attcgaggat ttccttttcc gcgtccgaat accacaggcc gctgcctccg atgacgatgg
      121 gatttttggc cgctttgaga agatcgacaa ccttccgggt gttttcgcgg tcaaccggct
      181 tggatgaaag ggttgtgccg ggcttcttga ccgcggccat atccactttg gcgttcagaa
      241 tgttgcaggg aaactccaga taaaccggtc ccggtcgtcc gctcatggcc gcgcgaaagg
      301 ccatgtcgat atactcggga atgcgttccg gcatgtagca gaccagcgct tttttcacca
      361 tcggttcgat gacgggaagc tgggtcatgt cctgcaggtc cagcttttca acgctgtcga
      421 gtcccacgca tccggaaatc agaatcaccg gcgcgttgga cagccttgtg ctggcgatgc
      481 cggtgagcgc gttggtgaat cccgggccgg ccgtgaccat cgccaccgcg ggtttgcggt
      541 tgatcctggc ccaggcctcg gccatgaaga ccgccgcctg ctcatggcgc acgtcaaaga
      601 tggtgacggg tgagttttcc agaaactggt aaatgggcgt gatgtgcccg ccgctcaacg
      661 aaaaaacata atccacgccc cgttcgacca gggcgtccgc aacgagcttg ccgccgataa
      721 tgttgtccat aatatatcct cctaaaacag gtttattgtt cattgtgcgc agggaacggt
      781 cgcgacggtt ccctacagtt ccatgcactg gcctccatca aggttgacgg cctgtccggt
      841 aatgtaccgg gaggcgtcgg acgccagaaa agcaaccagg tcgcccgcgt cgcggggcag
      901 gccgatatat cccagcggga tggtcttgca catttccttt tccctctctt cgatcgttgt
      961 gccgaaaaag gacgcctcca ggccgaatcg ccatttttcc agatccgtgg caatctgtcc
     1021 cggacagatc gcgttgaccc gaatcccgcc cccgcccagt tctttggcca tgactttggt
     1081 cagcatgatg acgcccgcct tggccacggc gtaggcgctg ttgaagatgg gaggcacctt
     1141 gccggcccgc gaggccgtgt tgatgatcga ggcaggtttg cccgccatca ggggcagaac
     1201 ggcccgcgac atgcgaaaaa cggaaaacag attcacatcg atcgttttca tccaggccgc
     1261 ttcgtcgtaa cccagcaccg tgttgggaac gccgaaggac gctcccgcgt tgttgcagag
     1321 aatgtcgata tgatcgaagc tttttttgat ggtctccacc attgcgccga tggcctggtt
     1381 gtccgccaca tcgacggcga cggccagggt gcggacagca tatgctttgg ccagctccga
     1441 agcgatggct ttcatgtctt cgctgacgac cggctgcagg gggctttccg gcgcgggcgg
     1501 cgcgcccaga tcggcaataa tcacattgca gccgcaggag gccagcttct cggcgatggc
     1561 gtagccgatg ccggtttttt tgccggcccc ggtgaccagg gccgtcttgc ctttcaaatc
     1621 attataaatc atcgcttcct ccttcatcga cccagcggcg atggtccaga tgggcgtact
     1681 tggacaggaa gcgcgtgggc agggccagcg cgtccttgcg gaaaggcggc gcaagctgct
     1741 tgccgtccac cagaaattca agcacggtgg gcttgcggct tttcaggcag gtttggaagg
     1801 cttcccgaat atcctgcgaa cggctgacct gcatcgcatg ggcgcccatc gctttcgcta
     1861 ccgggacaaa gctttccggg ttgggaatat cgacgccgat aaaacggtta ttgtagaagt
     1921 ccacctgatt tttggcctcc gcgccccacc acatgttgcg aaagacgcag gcgatcacgg
     1981 gcaggttgtg ctctacggcg gtgctgactt catgaaggct cattccccac gcgccgtcgc
     2041 cgatgatggc gatcaccggc gctttggggc gggcgatctg cgcacccagc gccgcggggt
     2101 aggcaaagcc cgtgttgccg aaggtcagcg ccgcgatatg ccggcgcgga tggttgaatt
     2161 tcaggtagct gttggccgtc gaagagacat ttccgatatc ggtggtcacg atggcgtcat
     2221 ccggcaacgc ccgcgacatt tccagaagaa cacggcgcgg gttcatggga ttgccgtcca
     2281 ccatggccag atcaacaatt tccttttccc atttctttct ttcccgggcg acggccgcca
     2341 ggcgcttctc gtccggcttg cgcctgccgt ccatcgcttt caggcgcttc agaatttccg
     2401 cggaggcttc ttttgcgtcg ccgatgatgc cgacttcaat gggatgcgtc cgggcgatgt
     2461 gcttgggatt gatgtccacc tggatgatct tcgcgttttc cgggaagtaa ttgatgtcgt
     2521 actgcggcag cgttccgaag acggaaagcc gcgtgccgat ggccagcatg acgtcggcct
     2581 tggcgagcgt cttcatggcc gccttggaac ccatgtagcc gatgggtccc acggacaggg
     2641 gatgatttcc gtaaaacgcg tcattgtgca gatacgtcac cgctaccggt gccgtcagat
     2701 gttcggcaat ggctttaaca atatccagcg cgtcggcatc caccacgccc cgtccggaaa
     2761 cgatgaccgg gtttttcgct ttggacagaa gctcggccgc ccggttcagc tgttccatgg
     2821 agccgcagcc gcgcgcgtcc acacggtact gcgaaggctt tagaatcttt tcttccagtt
     2881 cgccgtagaa ataatcgcgg ggtatatcat acagaacggc gccccggtcg gcgtaggcaa
     2941 tgcggaaagc ggtgcgcaga cagtcggccg cccgtttcgg atgggggacg cgaacgacgg
     3001 ctttggtgac ggacttgaaa accgacatct gatcgcattc ctggaacccg tcccagccga
     3061 tggtcggcgt tcccgccgaa ggcgaaatga tcaccagggg tgtgtggccc atattggccg
     3121 ccgccaccga tgtaaccatg ttggtgatgc cggggccgtt ctggcccgtg acgaccccgc
     3181 agcggcccga tatacggcaa taggcgtctt ccatgtgggc cgcgctttgt tcgtgacgca
     3241 ccggaatgaa tttaattccc gccgtcggga aaaggtccag catgtccata aaggccgaac
     3301 ccacgatgcc gcagacgtgg tccacgcctt cggccagcag ggtttcgaca attgcctcgc
     3361 tgggtgtcat tttaactttt ttcatctttt tacctccaaa gtattctatt ttgactcctg
     3421 ccgaaaattt ctttttttaa tggatcattt tgtcaaccgc cgggccgcag ggggagccat
     3481 ccgtgccgcc gcgcacatgg acaaaaattt tgtaaaagac cgtgagaaga aaaaatcccg
     3541 ccgcccagac gcccagagtg atcgtgatct ccggccctgt cgggtggtat tcggtgatcg
     3601 ctcccagcgg agacggaata aagccggtaa tgaccaggcc cagacccttc tcgatccaga
     3661 gcgaaacaaa aacggcgatg caggccaggg ccagccagac ctcattccgg cgaaccccgg
     3721 gaaggatcag catgacaaaa gcggcaatcg atagaacggc aaagatccac atccaggcaa
     3781 caaggttgtg atgcccttcc agccccatga agaggtactg aaaatgatgc atatgctccg
     3841 gaatctgact gtaaaacacc gtaaacatct ccacgccgac aaagaagatg cttacggcca
     3901 gcgcgtaggc gacgatcttc gccagcgcct ggattgcttt ttctcccgcg tcgaatcgcc
     3961 cccagcgccg aagaatcagg cagaccagga tgaggagcgc aggccccgag gcaaaagcgg
     4021 aggccagaaa gcggggcgcc atgatcgcgg tgagccagaa gctccggccg ggcagaccgg
     4081 cgtaaatgaa agcggtgacg gtatgaatgc tgatcgccca gggaatcgac aggtaaatga
     4141 ccggtttgat ccagccgggc ggcggaattt ctttttttgt gcaggccaat atgacccagc
     4201 cggtcacgag gttgatgagc aaatagccgg aaaggacaat catgtcccag aagagaatgg
     4261 acgagggctg cggatgcaga agcacgttca tcacgcgggc gggccggccg agatcgacga
     4321 aaacgaaaag aatgcacatg acgacggcgg caacggccag caactcgccc aggacggtga
     4381 ttttgccgaa cgccttgtag tcatgcagat agtagggcag caccagcatg accgccgagg
     4441 cggccacgcc caccagaaag gtaaactggg cgatatagag cccccaggaa acgtcccggc
     4501 tcatccccgt gatgcccaga ccgtaatcga gctggcgcag gtagaagaaa aatccggcgc
     4561 agccgacaag gatcagacag gcgatccaga tccggtacct caggctgccg tgtaaagctt
     4621 tttccagcat agcgtcctca taacaaatag taaatctgcg ggccggtgcc caggtgcaca
     4681 cgcctttgca ggctgaaacg cgtcttcaaa agacgccgga tttccgagcg gggattctcc
     4741 gcgtcgccga aggtaagcgc tttttgccgg cacgcctcca cgcaggcggg cagcgttcct
     4801 ttggcgagcc gctcctcaca gaaattgcat ttttccacga cgcctttgga acgggtcgga
     4861 aagtccggat tgattttagc gatacatggt ctcgggtcgc ggtaattcat gctccgcgcg
     4921 ccgtaggggc aggcggccat gcacagccgg catccgatgc agcggtggta gtccatcatg
     4981 acaatgccgt cgggccgctg aaacgtagcc ccggtcgggc acacgcggac gcagggcgga
     5041 ttttcgcagt tattacagaa aacgggagga cggagctttt gcagcttctc gccggaatag
     5101 gcgttctgtt cgccgggaaa gaccgaggca aaagactctt tccagatcca tttgatttcg
     5161 tccttgggat tgccgaagtc cggtacattg tgaatcgcgt gacaggcggc gatgcagtcg
     5221 gtgcagtccg ccgtacactt tgtcagatcc accgtcatgg cccagcgtcc ggccgcaagc
     5281 gccttcttga gcgcaaggcc ctgccccgga ggaatcacga tgacgtcttt ggcccaggcc
     5341 gggacaaaga tcgcgggcgt catcatcgtc agtgcggacg ctcccgcgat tttcagaaag
     5401 tctctgcggc tcgacgtcat tttttcacct ccgcgggaac gatgtggcag tcaaagcagg
     5461 agggagccgc gcccacgtaa tggtggcagc ggtcgcagaa atgctccttg ttgctgtggc
     5521 actgaaggca gcttccggtg aggctctttt cgaacgtccg tccgtcattt gccgtgtaca
     5581 ctcgctttcc ttcgcggacg gcttcatcgc gccaggcctg gagcattttc atgtggttgg
     5641 cgcgcatgaa gacggcgtcc tcgacgcact tcttgtccgc caatccggcg atctcaggcg
     5701 tctccaggct gagaacgggc ggggccgtct ttcccttgcc gtaccagaag ggaaacgtga
     5761 ccgcgattgc aaagatcgcc agtcccagaa gtatcaagcc ccatttatgt tgtttcatct
     5821 tcatacattc cgtaatatgc ttacgttcag cgacgagctg cgagctgaac cctatccacc
     5881 attcaccatc cactaaccac tatcgcctaa ccccatcgcc gtattttcac cctccccctg
     5941 cccctcccat caagggaggg gagtctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnntgtt ccttctgctt gtacccttca gggtatcaaa agagggggca tctgtagttt
     6121 cccctctccc ctcgtgggag agggtcaggg tgagggggct catatcgaat cccctcttaa
     6181 atcggtggtt ctctcctttt cgcctttcat caccagggcg ttggccagca gttcatgaac
     6241 gcccatgacc gtgacgccag gcacccagta gtccatcaga ggggggagcg tgacgcggtc
     6301 gatggcacag atgcagccca ggcggttgac gccgtgcttt cgaacgacat cccgaacggc
     6361 catggcacgg ggcatgccgc ctcgcattcg catctccata ttctcatcat ttccgagtcc
     6421 ggccccgccg ccgcaacaaa atgttttttc ccgaatggta tcctcggcca tttcatagaa
     6481 atggttgcag gacttttgaa tgatgtagcg cggctcttca aacagcccca tggcccgcgc
     6541 cggattgcac gaatcatgaa atgtcagccg gatgtgatcg ttgcgggacg gatcgagctt
     6601 cagttttccg tttttgatca ggtccgccgt gaactcgaca atgtgcacca ttttggtcga
     6661 gcgcgcattt tcaaagcgcg tgcccgtaat cggggagacg ggctcttcga gaaagtccgc
     6721 cggaccgttc atggtatcca tgtactggtg aatgacccgc cacatgtggc cgcactctcc
     6781 tcccagaatc catttgacgc ccaggcgttt cgcctccgcg taaatcttgg cgttgaggcg
     6841 tttgaccatc tcgtgggagg tgaacaggcc gaagttgcct ccttcagagg cataggtgct
     6901 gaacgtgtag ttgaggccga tttcatggaa gagcatcatg tagcccatgc aagtgtaagt
     6961 tccgggatcg gcaaaataat caccggacgg cgtgacaaaa agaatgtcgg cgcctttttg
     7021 gttgattggc acatccacgc ggacgccggt gcgatcctcg atgtcgtccg ccagaaactc
     7081 cagcatgtcc ttgatgccgc ccggctgaat gcccaggtgg ttcccggtcc ggaagcagtt
     7141 ggccgcgggc tccagcaccc agttgatgct gcagccgatc aggttgagca gttcgcggcc
     7201 gatcatggta atttccgcct gatcgatccc gtaggggcag aacaccgagc agcggcggca
     7261 ctccgtgcat tggtaaaagt agtaaaacca ttccttgagt acatcgaccg tcaggtcgcg
     7321 cgcgcccgcg agcgtcccga agcacatccc cgcctttgtg aagtagcggc ggtaaacgga
     7381 ccggatcagc tcggcgcgca ggacaggcat gtttttcgga tcgccggagc cgatgtagaa
     7441 atggcacttg tccgcgcagg cgccgcagcg cacgcagatg tccataaaca ggcggaagga
     7501 gcggtatttt tcgagccgct ctttcatgcc gtcaagaatg atctgtcgcc agttttccgg
     7561 aagcttccaa tcggagtcgg atggctgcca ggcccgcgga ttgggcatgt ccagggccgc
     7621 cagattcttt gcggcggcag cgtagctcca cgttcccgga cggaaatcga ccggagtttt
     7681 catccaggcg gtggccggcg gcttgtatcc gatcttcatc agttcttccg gtttgggaat
     7741 tttcgcgagc gacatggcta ctccttctcc accggcaggc cggccgcttt cattttatcc
     7801 cggaactcat cttcatattc tttataggtg tgtaccttca caggggtgtt ccagggattg
     7861 atatgacgca ggctgcgatt gttgttgatc atgttgcgcg tcgggctcat aaaaatgccg
     7921 ccaagatgca tcagtttgct gaaaggcagc caggcaatca ggataaagac gaaacaaagg
     7981 tgcatgtaaa ggatgacgct tccgtctttg ggcgcaaccg gcgcaaacgt tgcccagccc
     8041 atcgccatgg tcttcacggc gttgagatca accggaaaga aatggcgcat caaaaggccg
     8101 gttgccgcaa cggccgcaat caaaagcagt gcgaaataat cggcaaacag tgaaatgtag
     8161 cgcacctgcg gcaggaccgc ccgccgcagg aagagaaacg ccagtcccgc aagcagcgtt
     8221 gcgtcgctca aataaagcgc cggaacgccg atttccagaa agccgtccag cgcggaaaga
     8281 cccgtcacgg cgggaagaac cggttcggtg aaaaagcgca gatgccgaag gacgacgacc
     8341 agaagcgagc cgtgaaacat cagcccgaaa aaccacagcc atttggcgct gccgtaaacc
     8401 ggccggccgt ccacgatttc cgtttgcgaa tttctgaaca aagaacgaaa caaaaagatt
     8461 tctgcggcca tccggccgat cgcgcccagc ggccccgcgg gattatccag tggattgctt
     8521 ttgatccagg gcaggctttt cccctggccg caagtcgcgg gaatgcgaaa gggaacggga
     8581 gctttcgccc accggataac gcggtaaaca aaaccagcca aaaacagacc aaacgccgca
     8641 tagggaagaa tcctgaacca tccgggcgcg tccgcccgcc cggccgccgc ggccgcaagg
     8701 gcgattgcca gaatcgcgat gaaggacagc aaaatgttca cgctttgttt catttcattt
     8761 gcccgttttc atttccatga cggctcctcg ccgaaatgga tgatcggttt tttcattcct
     8821 tcgagcgcgt tgaccatcag ttcaaccaga ccgccgtaat aaagatggct tcgttcttcc
     8881 gcatgataat aggcgatcat atcgcggatg gcgcctttgc agttggagca gggagcgcag
     8941 acgtacttgg gcgtttccgc cggctcgctt tcaaaagctt ggaggatctg cagaaacttc
     9001 tttctggaac tgacgaggtt gcgccactcg gcaaaattgt atcccgacat gatggcaaaa
     9061 ccggacccgc caccgcagca gtagttgttc acgccgtgcg gcgtcatttc ccggaagcgc
     9121 ggagcgattt ttccaaggat tcttctttgc ggcgccacga tgcccatcga ccggacgaca
     9181 ttgcagggat catgcagtgt gacgggaaaa tcattctttt gcggattaaa cgtcagtttt
     9241 ccgctcatca cgatatcctc aagcgtcacc agcgccgact cccgcggaat gttcaggtct
     9301 ccggtcagca aacggtcggc aacaaccgtc agtgccttgt gcgcatggcc gcattcaccc
     9361 aggacgatct tcttgacttt aagccgacgg gcaatgtcgg catgtttgac ggccacacga
     9421 gcaaattgaa cgtcgtcgta ccaaaggccg tagttgacgg cgtcgtagcc ggccgcttcg
     9481 gacgacagcg tccaggacag ccccgccgcc tggaagagga ttgcaaacgc tccgacgttt
     9541 tccggccagg ccagcatttc gcccgcgtta tgcagaagca gaatatccgc tccttgcacg
     9601 tcccagggga tttcgacttt gcagccggtg atctcttcca tttcttcctg aatgaattcg
     9661 acgctgtctt ttgccgccag cggattcatg cccgtcgaag atccggcccg cagatgcaga
     9721 acggtcccct tctcgtgaag ttctttgggc gcgatgccca tctcctgaga aaagagcttg
     9781 cggatctcgt gcgcaatgag accgttgtcc acgccgatgg ggcaaacgga cgcgcagcgc
     9841 cggcacagag agcagcggta ggaaagttcc gcgaggcgtg cgatggcctt ccagttcaag
     9901 tcgatgtcgc cggtccattt tgccgttatt cttccacccg gcttgagata ttttttatag
     9961 atccggcgca gcacttcgga gcggaaaatc ggccggtaaa tttcctgccg tccgctcatt
    10021 tcaaaaacgg ggcaggcctc cgaacaggtc tgacaccggg cgcaatgctc catggacaaa
    10081 agcaggggct gcaggaaggt ccagttattt tccttctcaa acagtttttt caggccggca
    10141 agaaatcggc gtaccagttc ctcttcttct tgcttgtttg cgggctcggc aatgccgata
    10201 gccagcgtgt cgtcgggcga cgtttcgagg cctttgatct tctccgccgc cagaggcttc
    10261 aaaacggcct cgttgaagtc ctcgtaaggc ggcggcagcg gtgcgagatc gcccggttca
    10321 atgcggcaca ggccggcgga gcctcttgag atatcggcgg gcgttatgcg gtctttcatt
    10381 tcatctcctt gggccccgcc gaagcgatat cctgccaggt tttctttccg tccgcgtcaa
    10441 cgtgcgcggc tttccatgtc ggccggtact gcagattcct ccgtatggcc gatgcgatgg
    10501 gtgatccggg agtgttcggc gaatcgtccc atttcacctt gtgatacaaa aaaaacttca
    10561 tgaacatgtg cgatagatgc gtcagcggaa tataggccgc aagaagcgag cccagcaata
    10621 tcgtaagagc tccaaagagg ctttgtccgg gaacgtaggc gggagcggcc gcgccggcgg
    10681 tcagcaggcc gagcacataa agcctggccg cggggaagca gggatcgacc gccagcgcag
    10741 agagaagggc acagacaaaa aacagcaaaa taaagacgag attgaaataa tcggccggcg
    10801 ttgcataggc ccggagattc cggtcggcaa ggcgtttgta cagcatgccc agggacccgg
    10861 cggtgccggc caggatgccg atccacccgg cggcggcgat cagcacattg atgatcgatg
    10921 aaacagcgct gtcttcggca agccccagca aaaacaggac ggcggacaac aaaagcagcg
    10981 caaaaaccac cagcatcagg tacattcccg cgtgaaaggg aaaggaaaac agccacaggt
    11041 tccgattttc cttccacaag ccccgcatga gcccgatttc cggcgccatg taaagaagtt
    11101 cgttcaccgg tgagcgctcc cgctttttct tccaccagtt cagctcttcc atgtaagaac
    11161 cgccgtaagc cgccttattt gccggttcgt gctgcaccgg gtagatttcc cagcgcacat
    11221 gcacgggaag cgtcagctgc cggtatacgc ggcaggcaac ggcaaccaga aaaatgaccg
    11281 tgcataagta tgaaaaaata taaaatccca tatcaatccg tcagtttaat atttcttttc
    11341 caaatcaaga tgataccgcg gcggcaagga ggaggcgccg gaggcgtatt tttaacacgt
    11401 cgaggacgcc gacaacgcag ccaacaaagg tagcgcttga tttcggaaag aaatcaaacg
    11461 cagcccgtcg gcttggacaa ccccgccagt ttgcacgctc cctttgccgg tccggtcggg
    11521 aaaagttcat aaagcttctt catatcgatc ccgttgtcct tgcagacttt gcgcaccatc
    11581 ggggcgatgc cgaacttcgc aaaatagtcg cgcaggtagt tgatgatctt ccagtgctct
    11641 tccgtcagcg gtccttcaat gtcttcggtg tgcgcatagg cctgcgcgaa ttcttcgttc
    11701 catttatcga gttcctgcag aaacccgtct tcgtccactt cgtaaatttt gcctgctaat
    11761 tcgatctgcg gcatggttaa aacctcctca tgatatttat cgtgcattct tcgtttcgtt
    11821 tttcttcgcg gtactgaaag gctttggcga tcatggcctt tgcccattca ggcgttccca
    11881 gcgcatgcac gtgggtaaag ccggccagaa tatttttccg gcacagccct tcgcgttgtc
    11941 cgtcgagccc cgtccccttg aggacgcgaa aagcggtgtg cgtttcctcc tggcggcgag
    12001 acagaatccg gccatagcgg aattcgtgtc cgttaatcgt cgtcccgacg ggaaagaaag
    12061 ggttttccgc gtcggcggca agggtagtgt aaccgtgccc ctgcggtttc gcttccatgc
    12121 cgaacgccac cggcagagcc ccggtcatgg gataccgctt gccgccgact gtcagggatt
    12181 ctcccaggta gagaaatccg ccgcactccg cgtaaatcgg caggccggct tcggccgcct
    12241 gtttgaggca gaggcggaac ggttcgttcg cggcgagcct gccggcgtgc gtttccggaa
    12301 atcctccccc gatgtaaagg gcgtcaacgg gcggcagctc ctcatcgtca agcgcgctga
    12361 tccggacgac ccgtgcgccc cggtcggcga gcgcttcgat attttccgca taataaaact
    12421 gaaacgccgc gtccgaaatc acgccgatgg tgacgggagg gtccatgtcc ctcacggacg
    12481 gagccgcttt cggaaacgct ccgaccttcc agggcggcgc gcttcgcgcg atcttcacca
    12541 gggacggaat gtccagatag tcgcgggcga tggcgccggc cgtgtcgagc gcctgctgaa
    12601 catcggcgtg ctccgcgggg ggaatcaggc ccaggtgcct ttggacaaag ggcagatccg
    12661 gcagacgcgg gacggtccca tagaccggaa gcccggtgga ttcttcaacg gccctccgga
    12721 tcacggacgc atggcgtttt ccgttcaccc ggcttaaaat cacgccgcgg atgtcaaccg
    12781 cgggatcaag atgcaaacag cccagcacca tcgccgccgc cgtacgcgtg actttgtcgc
    12841 agtctacaat caggataacc ggcgcataga gcagtttggc cagttcggcg gtgctgaatt
    12901 cgccggcggc gttgacgccg tcaaacaaac ctcggttgcc ttcgatcaga gaaaaggttc
    12961 cttccgcggc atggcgcgaa aaagatcgga ccacctgcct ggctcccatg agatacgtat
    13021 ccagattgta gcagggaccg gatgccgccc ggccaagcca ggcggcatca atataatccg
    13081 gtcccttttt gaaaggagtg accatcaggc cctgccggcg cagagcgacc gtcacagcca
    13141 aagaaagggt ggtctttccc gcgccgcccc gcagggcggc catgatgaag cgcgggcgtt
    13201 gttgattcat atccgttacc tcttgtaaaa cagtccctat tcctttggaa agaattccgt
    13261 caggatgagg tacgtcgagc tgccgctcga ccagtacttc agcacttcct cgtcgaggag
    13321 ttccttcacg gtgttttgaa tggcgtggcg gtcctcgccg ggcagccatt tggtgacgtc
    13381 ccgaatgtaa aacatttttt tgtcgccctt cgatttttcg cgaaacagct ccgtaatttt
    13441 cgccttcaga tcatcactca tggctacctc catttaaact gggcggacgt ccggaaagtt
    13501 tccgtcgcca gatcaaagtc atcgatatgc tgatccgtaa acggaatgcc cgtcaggctg
    13561 aagaaacgct cccagccgac gcgctcgatc cattcgccga cgcgctcgtg tttccgggcg
    13621 tggctcgcat aaacctcgat gatgtttttt accgcgttca ccacttccgg ccagcgcggc
    13681 gggttgttgg gcaggaaggg aatcgccagc ttggaaaaca tcggagcgct cctcgcgttg
    13741 gaaactttgc cgccgaccca gatggagatg ccgtcattgt ccggatcggc aatcggcatg
    13801 gccgggcaga cggtgtagca gttgccgcag tacatgcact tctcctcgtt gacggtcacg
    13861 gtcttgtttt tcatgtcgcc ccggatcgcg cccgtcggac aagacgccac cgtggaggga
    13921 atttcgcaga cattcggcac gcgcgagttg tcggtcttgg gaaccgtccg gtgaatgccc
    13981 agaatggcga tgtccgagca gtggaccgca ccgcacatgt tcaggcagca ggccagggca
    14041 atccggacat gcgccggcag cttggcctcc acaaagtgat cgtaaatttc atccatcagc
    14101 gctttgaccg gaccggaagc atccgtggcc ggggtatggc aatggaccca tccctgcgta
    14161 tgaacgatgt tggagatcga atgtcccgtg ccgcccacgg gatagccgcg atccttcagt
    14221 tccgcgatca gcggttcgac cctgtccttg tcatcgagaa gaaattcaat gttgtgacgg
    14281 ctggtgaagc gcagatagcc gtcgcagtat ttttccgcga tgtcgcagat ttcacggatg
    14341 tgatccgttg aaatcaaccg gggcgaaccg gcgcgcacgc tgtagagcgt ttctcccgac
    14401 ttcgacacat gcatcaggac gccgggcttc aggatttcat ggtagagcca gtccccgtaa
    14461 tttctttcga ttacgggagg cagcatcgtt ttatagttcg gggggccgat atcagttctt
    14521 gccgccattt tacttcacct cctttttcag atcttcgggc cagaagaaca cgtagggatt
    14581 ggttctcggc gctttaacca tctggggcac ggcgggaagg tccacggctt tcaaaaagac
    14641 acgcatgccc aggcgctgga tcagctcacc cagccgttcg cgcgatttcg cgttctcgtc
    14701 ccaccagtcc tggatccgtt cgatcagatt cttcaattcg tcataaggcg gttccagctt
    14761 tatgaacggg acgatcaccc aggagagcag ggcgccccgg acaatgggcg ctttgccgcc
    14821 gaccagaatg gacgcgcctc tttccttccc cggccgcagg gctttcggca tcagattgat
    14881 gcagtgcatg cagcgggtgc agtcttcatc gtatatcttc agctctttgc cgttccattc
    14941 catgcaccgg gtggggcaca gatcgcagac ttccttttgg atgttgatgc ttgaggcgta
    15001 ggccaggact tctttctgat caacctgaat gtcatctttc caggttccga tgaccgaaag
    15061 gtcggagcga gcgattgcgg ccacgcagtc gttggcgcag gcggaaatct tgattttgaa
    15121 tttgtaggga aacatcggcc ggtgcatctg atcctgatag gtcatcgtga gattgtagca
    15181 cagatccagg ctgtcgatgc aggcccactc gcaacgcgcc ggacccacgc aggcgctggg
    15241 ggtgcgaagc gccgaaccgg agccgcccag atcgaaaccg gcattcgaca gatcgtcata
    15301 gcaggattgg agatgatccg tggtggtgcc gagcagtatg gcgtcccccg tggagccgtg
    15361 catgttggtt agcccgctgc cgtgcttgtc ccagatgtcg cagagtttgc gcagggtgtc
    15421 cgttttgtaa aaccagcccg tcggatgatt gacgcgcatg gtatgaaacg cctgcacgtt
    15481 gggaaataga tccgggcggt ccgaatagcg cccgatgact cccgaaccgt agcccttgac
    15541 gcctacaatg cctccgtgtt tccaatgccc gatcctgtct ttataggaaa gctccagttg
    15601 atggatcagg tccctggcca tgggattttt tccggcggcc tgcttcaaat ccttcacaaa
    15661 gcttggccac tttccttttt ccagctcgtc caggagcgga gtttctgaat ttgccatttt
    15721 tgcctccttc atcaacgcat tttataactt aggagcatag actttctgct caatagcact
    15781 actcacaatt cgaatagtac tctttttatg agatgtgtca agtattcttg ttatgttttt
    15841 cggaaaattg ttaaacacta tccaagcatc tgaaaaatat ataaaaaaat aataggcatg
    15901 actggctttt actcaaattt gatgagcaaa aaccagtcat gctcgatact gatgaaccgt
    15961 tacgttttaa tctcgccggg tcatattccc cgaagcttgc ttcgttcctt gccgctatag
    16021 atacctcgta gcttgctgcg aggtagttta ttggccggca gggaaaaatg tataacttac
    16081 ccaaaccagt ccgacaagca gaaccactcc gagaatgaga ctccaggtga tcaatttctt
    16141 ttcaattggc aggaggggct catattccat tttttccatt tcgctcttaa ttttaacatc
    16201 atccatgata agctcctttc agtgtgaatt attgaaccag cggcggcatc acaccctgga
    16261 agaatatcca tgatatgaac agccctaccc agatgatgaa accgaacagc gcgatcacat
    16321 aaaccagcat cagttttccg atgccttctt cccagagttt cttaaagttg gaagccagcc
    16381 cgatggagaa gaaagtcaaa gcgaaaaaca gttttcggaa tacgtcggtt tccgaggcgg
    16441 aaatttttat ttgcgacttg atgtccggcc agttcaaacc gataatgagc atgatgatga
    16501 aagtcacaaa atagccgatg acaaatttgg ggaagcggtc ccatatttct ccggccctgg
    16561 ctttgtcacc cttggtggga ttgattttgg ttgtccagat gacggccagg atgaacgccc
    16621 atatgccgat gaaaatgtcg atgaaaattt taacagtcgt ggccgcgcct acaacccagc
    16681 ccggctgata attgatgccc tgggccgcgt ttttggcata gatcagagat tccacgatct
    16741 ggccgctggc gacggcggct ccgtccgtct taacggcaag gcccatccat gcccctgcaa
    16801 ccagcggctg gttgggaagc agttctgttg cgacaaacgg aaggatgatc agttcaacga
    16861 cggcaaaaat aacgaccaga gaagaaacca tgacgggaat gatcggacgg gcgcggatgg
    16921 cgccgcctgt ggcaatggcc gccgacacgc cgcagatgga tatgccggaa gcaagcggcg
    16981 ccgcccattc acggttgaac ttgaaatatt ttctggcgat taaatagacg acagcccaat
    17041 aaatcagata ggcttcgaca atggcgcaaa gtcctctgaa catcatcgat gtcgccagcc
    17101 ccagttgttc ggcggatttc acgccaagtc cgacgcccat aataacgatg gctgttttga
    17161 taaaccattc cgcccttgcc gcctctttca gtgagttggc aaacttcggc ataaagttgc
    17221 cgataaaaag accgaccagc aaagccagaa tcaatccggc ttcaccggtc agcttcaatg
    17281 accagtccag tccgaacttt ttcaggtcag cgggtgtctg cgcggcgatg accgcataat
    17341 gtccaatgat attacagcaa taggtcaacc agaaaataat cgtaaagcca taaacgaatt
    17401 ttttcaaatc ataacccatg gcccttgcgc cgaccatgag caatcccaga acggcaagaa
    17461 atgtcaaaat gatggatatg atcggatgga gttctgcata gcccttggat gcgggggcta
    17521 tggattttga aatttccatc cacactttag gcgttacggc ccatcctaag agatccaggc
    17581 ctaaaagcga gccaagtgag attatgaaaa taaataatcc cagccacact gaccaccagt
    17641 cctcggtctt gaaaagacct tgcttgctgc ccatagagac ctcccttttc attgattttt
    17701 agttctgaaa tacgaccggt aatgtttcac cccccctccc aaattatcgt taaagaagca
    17761 ttttaatcat tcagacctaa aaccattgtt aattgaactt tgagcgagga caatttttcg
    17821 tttccggttc attgctgagg atgtttaaga tatggaggcg gagattgctg atggagctga
    17881 aaggattatg aaaagccgca ggaagggcaa gatcgaattc aaatttaccg ctgaatatcg
    17941 atgccacagg aatcgtcatg cgacatctcc aaagggatgt tgagttcggg gatcgcagca
    18001 tcctttttga gtcgttgttt acgattcgga gtataaaaga ctcaatcttg catgtcaaga
    18061 aaatataggc gacagaaaat ataggcgaca atgtaggtga aaatataggt gaaaatacag
    18121 gcgtggccga tattttatct tgacgttttt ttcgtccgtc tatacattct aaaaaaacaa
    18181 ttcatggaaa acagatgatt gaaaatctga tcaacaattt agccttgtac atacagggct
    18241 cggtgatcct ggcttttgcg gccgcctacc tgggaggcgt ggtgatcagc ttcacgccct
    18301 gcacataccc gctcatcccc gtcacggtgg gattcatcgg cgcccagaaa gcaacgacga
    18361 agctgcgcgg cttcttgctg tcgctgtttt acgttctggg gctttcagtg acgtatgcgg
    18421 cgcttggcgc agttgccgct ctgtcaggcc ggctcttcgg tcagatgcag acgacgccgc
    18481 ttgtttattt ccttatggcc aatgtctgcc tcgtcatggg gctcgccatg ctggacgttt
    18541 tcaaaatatc gattccgatt ccgcaaaaaa tcctgaacta cacgggcggc gggaaaaaag
    18601 gatttctccc cagttttctt ttgggcgccg cttccggctt cgtcatcggt ccctgcaccg
    18661 cgccggcgct cggcgtgctt ctgggattcg tcgccctcaa gacccacgtg gcgatgggca
    18721 tcggcctgtt gtttgtgttc gccttcggca tgggaacgct tctgatcctg gtcgggacgt
    18781 ttgccggact gatcgccgcg ctgccgcggt ccggcgcgtg gatgacagtc attacaaggg
    18841 ttttcggcgt tattttaatt ggcgcggccg aatattttct gtatactgcc ggcacactgg
    18901 cattttaaca aggagcatca tgcaaaagat atatcgcgtt ttcgtggctt tgttcttgat
    18961 tctcggcctt tccgccattc cgtctgccgg gcaatttatt cccaacgcgc ctttcaagcc
    19021 gcaatatacg cccgcgccgg acttcaccct gaaggatctg aaggggaaaa tattccgcat
    19081 gagcgcccag cgcggcaaac ccgtcctgat ttttttcggg actacctggt gccccggctg
    19141 ccgggcggag atcccgaaat acaaaaaggt ttatgaaacc tatgcgtcca gggggctgga
    19201 agtgatttac atcaacatca tggagccggc caaaaaagtg gcgcggttcg cccaggccaa
    19261 cgctctgccc tatcggacgc tgctcgatga ggacggctcc gtcggcgcct tgtacaacgc
    19321 ggtcggcgtg cccatgatga tcctggtgga caaggacggc aatatgataa aggtcggttt
    19381 cagctccacg cagatgccgc tggacaaagt gacgccggtg caataagata cctgcgtgct
    19441 ttgcttcggc gcctgactcc gcaagcggag cccccgacgt catttacccc ggatcaagga
    19501 tggacccatg acaacggata actggtttgc cgaatttgcc aaatccgaaa ccgatcgctg
    19561 gatcggcggc gtctgcggcg gtttgggaga gcatacgccc attccttcct gggtgtggcg
    19621 gctgttgttt accctgctgg tgctgtgttt cggcacggga tttcttctct atattctgct
    19681 ttggatcttt gttccgcgcc gcgccgcaaa ccggccggga tgacgttcgg gaaaaaacaa
    19741 ctgcataaat ttacctaaag gggaaagtgt gtaaaatgaa gtaccgggaa gacatttatg
    19801 tttacgaatg ggccaattat ttcgacaaca actgcaacag ctactatatc ggcggcaacg
    19861 tcaaggcgct cgtcgatccg gggctcacgc gctacctgcc ggatttgctc aaccagatgg
    19921 ccaacgacgg catcaggaaa aaagacatcc aatatgtcat caacacgcat tcgcaccccg
    19981 atcacttcca ggggtccgag cttttcgacc atgaaaaggt gaagattgcc ctgcaccgca
    20041 aggaaatgga ttttctcaag ggcatcggcg gcgaacttta cggccttttc ggcatgaccg
    20101 ttccgcagat gcagatcaat atgcagctcg aggacggccg gctcgaactg ggcggcgggc
    20161 ttttcaaggt catttccatc cccggccaca cgcccggctc catcggactt tactggcctg
    20221 caaagaaagc gcttttttcc ggcgatgtcg tctttcagca gaatgttggg cgcaccgatt
    20281 ttccgggcgg caacggcgcg catcttaaaa aaagcatcgt gacgctttcg gatctggatg
    20341 ttgaaatgct cttccccggc cacatgagca tcatcgaggg ccacgacaag gttcaggaca
    20401 acttcaacat tatcatccag aacatattcc cctacattta ggagttctca attcgcggga
    20461 ggatcggcgg cgcatggcca atgaagggaa caaagtcatc tattccatga tcggcgtgag
    20521 caaggtttac gaaaagaaac cggtgctcaa ggacatttac ctgtcctatt tttacggcgc
    20581 gaaaatcggc gtgatcggcc tcaacggatc gggcaaaagc tcgcttttga aaattctggc
    20641 cggcgtcgat gcggagtttc tgggcaaaac cattttgtcc gccggctaca cagtcggcta
    20701 cctggagcag gagccccggc tcgacgaggc caaaaccgtg cgccggatcg tggagcaggg
    20761 cgtccaggcc acgatcgacg tggtgaacga ctacaacgcg gtcaacgaaa tgttcgccca
    20821 ggaaatgtcg gacgaggaga tggccaagct ctgcgaccgg caggccgccc tgcaggaaaa
    20881 gatggacgcg ctcgacgcct gggatatcga ttcgcgcctg gagatggcca tggatgcgct
    20941 gcgctgtccg cccggcgata cgcccgtgca agttctttcc ggcggagaac gccgccgcgt
    21001 ggccctgtgc cgcctgctgc tgcagaagcc cgatattctg ctgctcgacg aaccgaccaa
    21061 ccacctggac gccgagtccg tggcctggct ggagcatcac ctgcaaaaat acgaaggaac
    21121 ggtgattgcc gtgacgcacg atcgctattt tctggacaac gtcgcgggct ggattctcga
    21181 actcgaccgc ggccagggca tcccctggca gggcaattat tcctcctggc tggaacaaaa
    21241 gcaaaaccgc ctgaaaaacg aggaaaagac ggaaagcgcc cgtgtcaaaa ccctggagcg
    21301 cgagctggag tggattcgaa tgtcgcctaa aggaaggcat gccaaatcca aggcgcgcat
    21361 caaggcgtat gaggaactgc tggacaggaa tctggaaaaa gaatccggca cgcgcgaact
    21421 ctttatcgcg cccggtcccc gcctgggcga tctggtgatt gaagccgatg ctgtctccaa
    21481 aggatacggc gacaaactgc tggtggatgc catgaccttc aaactcccgc ccgggggcat
    21541 tgtcggcgtc atcggcccca atggcgcggg caagaccacg ctcttccgca tgattaccgg
    21601 ccgggagatg ccggatgcgg gcgtgatccg catcggcgag accgtcaaac tggcgtatgt
    21661 cgatcagagc cgcgacgcgc tggatccgga gaaaaacatc tgggagatga tttcggacgg
    21721 ccaggacatc atccctgtcg gcggccggca gatcaattcc cgcgcctacg tctcgcggtt
    21781 caacttctcc ggaacggatc agcagaaaaa ggtcggaacg ctttccggcg gcgagcgcaa
    21841 ccgcgtccac ctggcgcgca tgctcaagga aggcggaaat gttctgctgc tggacgagcc
    21901 gactaacgac ctcgacgtca acacgctgcg ggcgctcgaa gacgcgctgg agagtttcgc
    21961 cggctgcgtc gttgtgatca gccacgaccg ctggttcctg gaccgcatct gcacgcacat
    22021 cctggccttt gaaggggaca gcaaagtgct gtttttcgac ggcaactatt ccgaatacga
    22081 agaagaccgc aaaagccggc tgggcgcggc ggccagccag ccccaccgga tcaaataccg
    22141 tcagctcacc agaaggtagc cgcgggacgc gcagacccgc cgccgtgcgg cgccctgttc
    22201 cttgagaggg aggtggaaat gaaaaaaata agtttggcat tgatggcggc ggctctgctc
    22261 atttccggtt tgccgccggc gcaggcgcag gaatacggaa aggtccgcgc gctccaggag
    22321 cgggcggcct atgtgatccg gcagaaaaat gatttcgtcg tacgggtttt gcgttcctac
    22381 aagattccgc atgaggtcaa tgatcagggt gttgtcgttc gcatcaacat gaatgatcgg
    22441 tggatggacg tcacggccat cgaaatcgtt cccatgttga gggaagccga ggaccgaagc
    22501 cggcaggtgg ccgcgcacga gctgtttttc tttacggcgg acgggattct ggacgtggtg
    22561 tcggcgctca cgatccgcta gaagacttgc cgtcgccgct tttcagttcc gctagctttt
    22621 cccggtccag gtagtatttc ccgtccggcg ttttgacgac cagtttgtcc gttatcagaa
    22681 gatgaagggc ccgggggcgc aaattgcccg ttccgacccg aaagaaattt ttccgctcat
    22741 cctgaagccg gatggcccgg gacaccttga aggcgcccgc ctcttccagt tcggcgatga
    22801 ttttgaagca gacccgccgc aggccgaaac ccgcgaaata catcaccgcc aggacgaaga
    22861 ccgcaaagcc cacgacccac agaataattt gtatataaac aggcatatga ctattttcat
    22921 cctgccggaa gcgaggatcc cgcgcatccg gattctttgc attgccgcta gatgaacttc
    22981 acttcacgcg tcgccttcgt gaccacaaag cagcgctcga cattggattg agcgtccgcc
    23041 ggaatgacga aaaacccctg tcggttcaac tgatacccct gggtggttcc caccaggacc
    23101 tcgccgtcct tgaagagcac ccggatcttg cggcccaaaa cgggtatgcc cggctcaaaa
    23161 ttcttccggt cctcatgttt cggattgccg gagaaatcct tgacgaaaaa aatggctttc
    23221 agatcggcga cgcgcacttc cagcggcttg tcgctgggcg aaaatcccgc cggcgtgaca
    23281 tgaaaaatat cccggttggg ggcaaagtca accgtgacgc ctttgacaag acgcttgtcg
    23341 gtatagcgaa tgacgatctt gtttaacatg gcggctgggt tcctttccgg cgtgagccgc
    23401 gtttcaacgc gattttttac cggacatcgt tgctttgctt acaccaaccg atggaacttt
    23461 gtccagtgca tcgtttgaag aactcttggc atgtttcgga aagttgtggt acaaaaagcc
    23521 cgctttttcg tcgacttttc cgaatccgga tactgctttt atccgaaacg gcaaaaaatg
    23581 tcgatgacga aatgacgggg ataagggaca acatgaacat tcaggccaat caggcaatcg
    23641 gcgtttttga ttccggcatc ggcggactga cggtggttcg ttccctcatg gaacgcctgc
    23701 cttttgaaaa cattatttac ttcggggata cggcgcgggt gccttacggc gtcaaatcgg
    23761 tggaaaccat ccggcgctac gcgctacaga tcgccgaatt cctgttgaag caagacgtca
    23821 agctcctgat cgtcgcctgc aacaccatgg cggcggtcgc ctatcaggcc attcgggatc
    23881 tctcgcccgt gccggtgctg gaagtgatcg aggccagcgc gaaaatcgcc gtcgcccgaa
    23941 cgcgcagcaa atccatcggt gtgatcggca cacccgccac gatcaactcg aacgcgtacg
    24001 ccagggccat ccatattttg gaccgggacg cgaaggtttt ttcgcaggct tgccccctct
    24061 tcgttccgct ggtggaggaa ggctggttcg agcatccggc caccaggctc gtcgccgagg
    24121 aatacctgaa gcccgtcatg gccgagcgcg tggacacgct ggtcctcggc tgcacacatt
    24181 atccgctcct caagcccctc tttcaggata ttaccggcgg gcaggtccgg ctgatcgatt
    24241 cggcggaagc catggcggac attgccgccg acctcatacg gcagaacaat ctgggcagca
    24301 cggcaaaccg gccgccggag tatctttttt gcgtcagcga tgtgccgtac cgttttcaga
    24361 ccatcggtga acgctttctc ggccggacgc tgcctcgagt ggaaatggtg aggcttcaat
    24421 gatgaaggtg ctgctggccg gctacaacgt cgattacgat ttgatccgcg accttcgtga
    24481 gaagagcggg ctcacccagg acattacgcc ggaaacgatt tccgccgcct acgcccgcat
    24541 cagccgcagt cccaaatccg tcgatcagct gcgcgttgac gcccgggccg aagtcgaaaa
    24601 agcccgccga tccaaccgga atatcgtttt tgaaatgggg cacagttcgg tagccgaaca
    24661 cgccgtcttc aatatcgacg tcatcggcgt ttcccgtctg ctggtcgaag aaatcgaaaa
    24721 gttccgcctg tgctcctata cggaaaaatc ccagcggtac gttttgtttg accgggactt
    24781 cgttgtgccc gaagaaattg tccgggcggg gatggagaag gattttgtcg aaacggtccg
    24841 gatgcaaaac gatttttacc atgagctcta cgagtggctt cggccctggg tctttgagca
    24901 aaaccgggaa ctggccgaaa atcccgccaa ccgctctctg ctggagggct gggccaaaga
    24961 agacgcgcgc tacgccatag cgctggccac ccggacgcag ctgggcatga cgatcaacgc
    25021 ccgcaatctg gagctgatgt tgcgccggct ggcctccctg ccgctcgcgg aggcgcgcct
    25081 ttgcagcgaa aagctctatg ccgcaacccg ggacatcgcg ccgtcgctga tccgctacac
    25141 ccgggcgacg gattacgacc gtctgacccg gccgcggctg cggacaaagg ccgaaatgct
    25201 tctggccaag cggcctcaag aggcaccatc gggaaagttc gccgacgttc gcctcgtgtc
    25261 tgcgacgccc ggcgcggacc ggcaggccgc cgccgcgctt ttgtttgcgt cttccggcct
    25321 gagctacgga cagtgccaaa gccgttctgc ccggctgaac caccgcgagc tgaaaggttt
    25381 gttccaaacc gtgtttgaca atatgcaacc ctacgatgca gcgcttcggg aactggagaa
    25441 tatcgatctg cagttcgagt tggtggtcag cgcgggctgc tttgcccagt tgaaacgcca
    25501 ccgcatggcg accatcatcg cccaggacta cgacccccgg ctgggggtga ccgttccacc
    25561 ctccgtgcgc gccgtcggcc ggcaaaagaa attcatgacc gtcatggagg cgacccaaag
    25621 ggcgcatgat cgcatccgga acaaactgcc gtcggcgccg ccgtatctgc tgaccaacgc
    25681 acaccgcagg cgggtgctca tgaaattcaa cgcccgggag ctttatcatc tttcccgcct
    25741 gcgggcggac gcgcacgccc agtgggatat ccgaaatctt tcggaaaaaa tgctccggca
    25801 ggcgcgccgc ctcatgccgc tgacgctgat gatggcctgc ggcaaggacg gctttgccgc
    25861 gctgcataaa aaagcctttc ccggaaagta accccctttc ctgtgacggg acccatggcc
    25921 actccgaaaa aactccccat tgttcttttt gccaccacga tcagctcgtt tacgacgccc
    25981 ttcatggggt cttccgtgaa cgtcgccctt cccgccatcg gccgggcttt ttccatggat
    26041 gcaatttccc tgagctgggt ggcgtcttcg tttcttctgg catcggccat catgctgctg
    26101 ccgatggggc ggctcgcgga tatttacggg agaaaaatat ttttcctttc cggatcgctg
    26161 atttttgctc tctcctcgct tctggccgtc ttgccggaaa cggagagcct ttttatcgcg
    26221 ttgcgcgtcg ctcagggatt cggcgccgcg atgatcttca gcaccggcac ggctctcctg
    26281 gtttccgcct atcccgcggg cgagcgcggc aaaatgctgg ggatcaacat tgccgccgtt
    26341 tacaccggcc tcaccgccgg tccgttgatc ggcggcctgc tgacccagca cctgggatgg
    26401 caggccattt ttatcgcaaa cgccgcgctg ggagcaggtg ccgcggggct ggccttgcgg
    26461 atggaggacg aaaaaacgcc cgccgcggcg ggagaaccat ttgacctgag cggttccttc
    26521 ctgtacgcgg ctgcactgtt tgccctgatg tacggctttt cgcggcttcc ctcttcccgc
    26581 ggcgccgttc tgattgccgc gggcggaatc tttttcatta ttttcatccg ccgtcagctc
    26641 caaaagccct cgccgctcat caatatccgc ctgtttctgc gcaaccgggt ttttgccttc
    26701 tccaacctgg ccgccctgat tcactacagc gcaacctttg cggtcacgtt cctgatgagt
    26761 ctttatctgc agcacgtcaa agggctcacg ccttcccaga ccggtttttt gctggtcatc
    26821 gaacccctgc tgcaggcggt cttctcgccg ctggcggggc ggctgtccga tcgccatgaa
    26881 ccccgcatca tttcctcgat cggaatgtca atcacggtcg tcggcctcgc cggtttgatt
    26941 ttcgttgcca gggatacgcc gctttggacg attctgtttt gtctggcgct tctgggaacc
    27001 ggcttcgcgc ttttttcttc tcccaacgtc aatgcggtca tgagctcggt ggacggcaaa
    27061 ttttacggca tcgcgtccgc gatgcaggcg accatgcgga tggtggggca gatgctgagc
    27121 atgggcattg ccatgctggt tttcgcctgg atcatcggca gccgcggcat tgactacgaa
    27181 aacggcgagc tgctgatcac gagcgcgaaa atcatttttg caattctggc ggtgatctgc
    27241 gtgggcggca ttctggcttc gctggcgcgc gggcgcgtac ataaggcttg attttcagga
    27301 cgtgatccgt atgatcgtaa acggcgatgc cgtttatttt tgtgaacggg ggttgaaaat
    27361 gaggacgcag tggatcagtt ttgccatcgg tcttgccgcc ggctgtttcg gcggactgat
    27421 tggaatcggc ggtggaatca tcatggttcc cctgctggtg gagatactgc atgtggatca
    27481 gtgccgcgcg cacggcacgt ccctggtggt attgattttt acgggaatca gcggggccgt
    27541 ctcttacgga ctgcacggcc aggtgaattg gaaagcggcc gttctgctgg cggttccggc
    27601 catgctgctg gccgcggcgg gcgcccgtct ggccgaccgg ctgcccgact ggaagctgaa
    27661 aaaatttttc ggcgggtttc tgattttttg ctccgcgctc ctgcttttga agcccattct
    27721 cgccgattgg atcggcgttc tgccggatgc cgtctccaat gtcgccctgg tcgtgacggg
    27781 gggcgctacg ggatttcttt ccggtctgat ggggatcggc ggcggaacga tcatgattcc
    27841 cgccatgatc cttctgggag gctttacaca gcacgttgcc cagggaaccg ctctcctggt
    27901 catggtccct tcaggattcg tcggcgcttt cgcgcactgg aagctgggca atgtggcggg
    27961 caaactgctg ttcagcatgg tgccgggcat tgtgctgggc gcctttgccg gcgccggaat
    28021 cgcccagttt attccggagg agcccttgcg ctggatgttt gtcctggtgg cggtttacat
    28081 gggatggcgc tatgtaaaag tcgtctcgcc gaatacgtgc tgaagcaccg gcttgcggtt
    28141 ttcaggcccc catgaacctt atggctgcgg cccgaacccc gtcgcctacg ccttcttccc
    28201 gtactccgga tcgatcttta ccagcgccgt gacgatgaat cccgggatcg tcgtgagcaa
    28261 tatccagtag tagaaatgaa tatatccgag tttttcctga atccagccgc tggacatggc
    28321 cggaagcatc atgcccagcg ccatgattcc cgtgccgatg gcgtaaaaga cggttttgta
    28381 ttcgccctgc gaaaccatga tcatatacat cgtataggcg gtaaatccaa agccgtagcc
    28441 gaattgctcg gctgctaccg cggaaccgat cagccagata ttatccggct gcgcgtgcga
    28501 cagatacacg aaaattacat cgggcacatg catgatgatc accattggcc acagccagaa
    28561 tttcaggccg ttgcggtaaa tgacatagcc gcccaaaagc ccgcccagca tgagcgccac
    28621 aacgcccacg gtgccgtaaa tccatccgac ctgggcggtg gaaagcccca ggccgccttc
    28681 ggcgcgggaa tccagaagaa acggcgcgac catcttcacc agttgcgctt cggcaaagcg
    28741 gaaaaacaaa aaaaagcaga tgatgacgac aatatctttg cggcggaaaa acagggtgat
    28801 gatccggaaa tattcagcca caacgccccg gcctttgtcc agcggcgcgc tgtggtcggc
    28861 ttcgggatag ggcagaacca gcaaatgata caggcagaga gccagaaaaa aagcgccgac
    28921 gaccacgaat gtgatcgacc aagcgcaagc tgccggataa ccgcattttt gcagcgtgcc
    28981 ggccagaatc accaggccgc ccttggacgc aatggtggcg acgcggtaaa aaacagtgcg
    29041 cacgccgaca aaggcggcct gttcgggcgt ggtgagcccc agcatataaa agccgtcggc
    29101 ggcgatgtca tgcgtggcgg aagaaaaggc catgatccag aacatggcga gcgtatagcg
    29161 cacaaaatcc gatgccggca gcgtgagggc gacgcaggca aatgatccgc cgatggccag
    29221 ctgcatggcg acgatccaga agcgcttggt gcggaaaaga tcgacgaagg ggctccacag
    29281 cggtttgatc acccagggca ggtaaagcca gccggtgtaa agagcgatgt cggtgttgga
    29341 aagccccaga tttttgtaca tcacgacgct gacggtcatg acgatcatgt agggaatgcc
    29401 ttcggcgatg tagatggacg gtacccagag ccaggggttg cgcttcgcat tcatgcgcct
    29461 gcctttgctg gctttgtcat cgtctcctcg gcgtatggcg ccatacgcct gcgtcgcctc
    29521 tttcccgccg cctcggcatg cttttgactg cgaagcgcga ccatcgtttg catgttgatt
    29581 gggatcagac atgaagttcc tttagttggc tcttgcaccc gttttccagg tcatccttga
    29641 ggacgtcaat acagtttttt caacattgcg ccggtaaaat aatgcgacag aatctcttcg
    29701 gcgctccgtc cctgagacgc catcaccgcc gccccgatct gacacaggcc cacgccgtgt
    29761 ccccatccgc cgccctccag aagaaagccg agcgcgtttc cgtccggatc ccgtttggct
    29821 gagacgacga aagcgctgga gagcaaatgc gtgggcgaaa gccagcggcg gatttccagc
    29881 tccctgccga caaccgccgc gccgagcgag ccttcgattt tcagccgaca aattcttccg
    29941 gacggaccgc gctgcagcgc ctcgatattt ttcagcctgc cgaagtcgat gccggatttc
    30001 gacctgataa tttcctccag ctcttcgggc tgatattcaa cccgccagcg atagaaattc
    30061 aaggtctcca gatcaaacga gggcagtatc ttccgcagaa tttccggatc gttggtgttg
    30121 cagtatgcgt cgggttttgc gcgcagccat tcggccgcgt ctttttccgt atggatcggc
    30181 gcgcgctgtt tcttgccgtc cgtgatgctt gtcagatagg ggaaagaggc atcctcccat
    30241 gcggttgcaa aaacatccgt ccgtccgccg cagcatttat agtagcgcgc atcgcagatt
    30301 tcacccgcgc tggtcagaaa tattccgcgt gtggcgtcga tggcccggtt gacattttcc
    30361 gaaacgatgc gggtgatgcc ctgatagcgc tgacagtggt cgtcggcgca cacgtcgaag
    30421 ccctgatgat cgttcacatc ctgccaaacg atgatttcgc cttttgtttt tggatggcgt
    30481 gcccggatgg attttttttt cgccagcatc gccgtcagcc agcttcgcgc ggtgacggcc
    30541 tgtgctttga gaaactccag aggggcgctg gccgccattt ccgaagaaat caccgaggcc
    30601 aggtattcct cgagcggaac gatattgatg aggtgaaagg cggattggcc ttgtcccgaa
    30661 agcaaaagcg ctccccggaa tgactgtttc tgcgcccgct gccagtgaaa atcgatgccg
    30721 atctttatgc cggatacggt aaaaaaagat tcagcgggcg cggcgggaga aagcagaatg
    30781 tctttgccgc ggatcccccc ccggccttct gtgtcggtca gaaaaacctc gccgccgtcg
    30841 gcgcggacca tatatccgcc tttgataacg cgcccgtcgg gaagaatgaa atcgccttcc
    30901 aggatcagcc gggcttcgga agtatgctgc agcagggccg cctgtatgtt cggttcgtga
    30961 tcgatcattc cctcatccca cagcgcgaaa agtcacaggc cttcttaaaa acaatagtgg
    31021 gataagtcaa gaaagttgtc ccggaccctg ctgtgatttt ccgggtgaag ggctgctcca
    31081 tgcggtcccg ccgggtgaaa aatttcaaac tgtttaaaat cattgacaat atcaaacttt
    31141 tttcttgaat cctgttcgtt tttcgtgtag gaaactacta aaatcattga gcggatggtt
    31201 tatggcgcat atttggccga ttggcggagg caaaggcggc tccggcaaaa gctttatcac
    31261 cagttcgctg ggacggttgt cggcggggct gggcagaaaa acgctggtga tcgatttgga
    31321 tctgggagcg gccaatctgc acacgctggt cggggttccc tatccttcga aaagtttgtc
    31381 cgattttctt cagaaaaaga cggaacgcct ggacgacacg gtcgtggaga cgccttttga
    31441 aaacctcttt ctcatcagcg gcgcccagga ccatttcgcg attgccaatc ttccccacga
    31501 acagaaggtc aagacaatgc gcgccatcat gaagctggat tacgactgca ttctgctgga
    31561 tctgggcgcc ggaacgtcat tcaacacact cgattttttt ctgatttccc aaaacggcat
    31621 tttcattacc acgccggagc ccacctccat tgaaaatgtg taccgcctga tccgatccgt
    31681 ttatttccgg cgaatccgtt acggtttcag cgtgtcgcaa ttcaaggcgc tggaggaaga
    31741 ggcgatcgag cagttcggcg agggcgcctc gggccggccg gagtttatga tgaacgtcat
    31801 cgcgcgggac tacccggagc gctttcggga acttcagaag gattttgacg ccttccggtt
    31861 caggctcatc atcaaccagc ttcacaggcg ggacaatccc gcccttggag cacatgtttg
    31921 cagaattatc gaaaaacaca tgaacctgca cgtcgaattc atgggcaatg tctcttacga
    31981 cgatcatgtc cacgatgcga tttgtcagca ggtgtcttat ctggaccgtt acccttacac
    32041 gaagaccgcg ggcgagctgc gcgccgcggg ccggcagatc tttcagccgg cgggccggca
    32101 attgatgctt cagtacgtgt agaaagagcc gggcttggcg cacaaccgtc ggggaggcct
    32161 gagtgcggaa aagttttgcc gagttgaact attatgaaat gctggacgtg agcccgaccg
    32221 cggcggcgtt tgaaattcgt caggcgtacg gcacagccct gcagatttac cggaccggtt
    32281 cggcggcgag ccattccttt ttcaccgagg cggaaagacg ggaaatcctt tctctggtgg
    32341 agaaagctta caggaccctg gcggatgaac tcacccggaa agcctacgac gacgaactgg
    32401 caagccgggg agaaatcgac cggccggcgc aagccgggcc cgacgccaaa aagccggtcg
    32461 gcatattcaa catcagccgc gtccgcgacc gtcagccggt cccggcaaac caggaatccc
    32521 tgaaaagcaa aatccgcgaa agcgaacgaa tcagcgagct gacggcccgc agttccctga
    32581 gcggagcagg cctgaaggag attcgcgagg agctcggtgt ggcgattgcg cagattgcgc
    32641 aggcgacaaa agtccggcag gaccatctgg taaacatcga agaagatcgc gtcgagcgtc
    32701 tgcccgcggc cattttcctc aaggggtttg tcaaatccta tctgaaatat ctctgtctgg
    32761 agccggtgga ggaattatcc gcccgctaca tggaaaccat cgcgcgtctg ctgcgaaggg
    32821 aaggggcgca agaacaggag taagaaagga gcggttatgg aatccgatac cattcaaacg
    32881 cccgaagggg atctggtcat ccggtttgtg gggcacagct cgctcatctt tttctgggcg
    32941 ggcaaaaaca ttcacgcgga ccccgtgtcg ctggaagcgg attatgcgag actgccaaag
    33001 gctgatgtga tattgatcac tcacgggcac ggggatcaca tggatgtgaa tgccgtgaac
    33061 atcatcaaaa tccccgcgac caaaattgtc ggcaacgatg aagtcggcag tcagatcccc
    33121 gggacactgg tgatgaaaaa cggcgaggag cgggtggtgg agggcctgaa gatcgaggcg
    33181 gtgccggcct acaacctgaa gcacatgaga gcgccgggaa agccctttca cccgaagggc
    33241 gtcggaaacg ggtatgtcgt tactctgggc ggtttgcgcg tctatattgc cggcgacacg
    33301 gaaaacattc cggagatgag acggcttcag aacatcgaca ttgcctttct gccgatgaat
    33361 ctgccttata ccatgacgcc ggaaatggcg gccgacgccg ctcaaatgtt tcatccgaaa
    33421 gtcctgtatc cctaccatca gggcgagacg gatacggcaa agctgcttgc gctgatgaag
    33481 gatcacaagg acattgacgt tcgcattcgg acaatgaagt gacaaagcgc gcgtcaaccg
    33541 ccgccggctg aaaagtttcg cgcgaggatt tagagacgga ggaatggatt caaatgaacg
    33601 gcaacggcgt tgcattctgg aaagcaagcg gcagcggcaa tgattttatc atcatcgaca
    33661 accgcgattt atcgctgaac gtgggggaac tgccggcttt tgcccgaagc gtgtgccgaa
    33721 gaaaagtatc ggtcggcgcg gacggtctgc tgctcgtcga gccctcgtcg gccgccgatt
    33781 tccgatggcg gtttttcaat gccgacggct ccgcggcggc catgtgcggc aacgcggccc
    33841 gctgtgtggc ccgttttgcc tttgaaaacg gcattgccgg acaaaccctg tcttttgaaa
    33901 cgcaggccgg catcatccgc gcggaagtgg cgggcgacgt cgcgaaagtc cgtctgaccg
    33961 atccctcttc cctgaagcag gatatccgga ttcatctgtc gggaggcgag tgcggactgg
    34021 actgcatcga taccggcgtg ccccacgccg tctgctttgt cgcgtccgcc gaggcgtgcg
    34081 atgtgacggc gaccggaagg gaaatccgcc gccatgaggt ttttcagccc gagggcgcca
    34141 acgcgaattt tgccctggtg atcaaccggc ataaaatgcg ggtccggacg tacgagcggg
    34201 gcgtcgagga tgaaacgctg gcctgcggca cgggcgtggt cgcctccgtt ctggctgccg
    34261 ccggacgccg ttttgtggat tcgccggtgg atgtgatcgt gcagagcggc gaaaccctgc
    34321 gcgtgtattt tacccgtcgg gacggggctt tttcggaaat ttatcttgaa gggaaagtga
    34381 aaatagtcta tcaagggacg ctttttgaag acgcatatcg ataatctctg atcaggaggg
    34441 aatgttcatt gtgcagacgg ccgaacggtt aaaaaaaatc cccccctatt tgttcatgga
    34501 gcttcggaaa aaaatcaacc aggcaaaagc agacggtgtg gatgtcattt ctctggccat
    34561 cggcgatccc gtggaagcga ctcccggttc cattatcgac gaactgtgcc ggacggcccg
    34621 cgacccgctg aaccaccgct atcccaccga cgaggaaaag ggcatgctcg cgctgcgcag
    34681 ggaaatcgcc cgctggtatg cccggcgcta cggtgtggag ctgaatccgg aaaaggagat
    34741 tctgggcctc atcggttcca aggagggatg ccaccacttt gtcctggcgc gcgtgaatcc
    34801 cggcgacatc gtgctgatga ccgatcccgg ctatccggcg taccgcgcca gcatcctgat
    34861 gggggaaggc gtcccctgca acgtgcccat cctgccgcaa aacgactatc ttccggcgct
    34921 ggaagacatt ccgtcggaga cggccaaaaa ggcgtcggcc atgtttttga actacccgaa
    34981 caatccgacg ggcgcctgcg ccacacagga gtttttaaac aaactggtgg cgtttgcgcg
    35041 cgaatacgac attgccgtct gctacgacaa tccttacggc gaaatcgttt tcgcagggca
    35101 ggagcgcatc agctttcttc aggcgccggg agcgaaagac gtcggcgtcg agctgaattc
    35161 cttttccaag ccctttaaca tgtgcggctg gcggatcggc atggcctgcg gcaatcccga
    35221 cctgattgcg gcaatcagca aggtcaagga aaatacggac tccggcatct tcaatgccgt
    35281 gcagtttgcg gcgatccagg cgctcaccgg cgaagcgggg ttcattgaag agatgctctc
    35341 catttacgga cgccgccggg agctggtttt gaaaacgctc caaaagatcg gcattgattt
    35401 tcagccgccg cgcggcacgt tttatctgtg ggtgcccgcg ccgaaaggca tgacgtcgct
    35461 ggaattcacc aaccgcctgt ttgacaaaac cgccgtcgtg gtagcggccg gaacggctta
    35521 cggccggtat ggcgagggct ttatccgtat ttcgctcacc gtgccggacg accggctcaa
    35581 cgaggcgatg gcgcgaatcg aaaaggaatt ctcgtaatgg aaatgggaat tctggggctt
    35641 ccccaaagcg ggaagagcac cctgtttgaa attatgaccg gcgtgaaaat aagcccgtcg
    35701 cacactgaaa cggtcgtgcg cggacaggcg tcggtgccgg atgagcgctt tgacaaactg
    35761 gtcgaaatct acaagcccgc taaagtctcg ccggccagag ttccgtttgt cgatgtgcat
    35821 gccgtgggcg aacatccctg ggaggcgatc cgccagaacc tgagcggcac ggacggcatt
    35881 ctgcacgtgg tggacggttt ttccctgccg gatatttcga ttgctctcga cgcctgccgc
    35941 aaactcgaag acgagctggt tctctccgat ctcatcatca ttgaaaagaa gctggagcgc
    36001 ctggccaaga tgtccaaaaa agccattgca ccccaggaag cggcgcaaaa cgaacttttg
    36061 ccgcggttga aggactgcct ggaggcgggc aaaccgctgc gccgggcggg gcttaccgct
    36121 caggaagtct ttacgcttaa gagtttttca ttctggacga tcaagccgga actggttgtg
    36181 gtcaacgtgg ccgaagacaa tccggcgttt gccgatgaat ttgccaaagc cgcccatctt
    36241 gccgaaccgg tgatgggaat ctgctgcaag atcgaggcgg agctcgcggg gctggacgcg
    36301 gccgatcaga aagagttcct ggcctcgatg ggcatcgccg aacctgcctt cggccggatc
    36361 atccgcgccg ccttttcgct tttggggcgc atgtccttct tcaccgtggg cgaagacgaa
    36421 gtcaaagcct gggtcattcc gcattccaca aaagccccca aggccgcggg cgccattcac
    36481 aacgatttcg aacgcggctt catcaaggcc gaagtcatgt cctttgacga tttcatggcg
    36541 cacggcgggt cgcaggccca ggtgaaatcc gccggccgtc tccgtctgga gggcaaggaa
    36601 tatgtcgttg ccgacggcga catcatcacc ttccgcttca acgtctgacg ggcgccgcgg
    36661 ccgcgtcctc caatgctttc aggtagtact ctgtttccgc aaccgatcga ttgaccagcg
    36721 gctgattcag tccggcgcct tccgaggaaa cgttcacgaa ccagttgttg tcgttgatcc
    36781 gctggaaacg gatggtgatc agcgtgttgc cgttaaagaa ggaatcccga tggattctga
    36841 tcacgccttc ctcatcgtcc ttttcgcatt tgtcgccggg gaatgttttc tttgccgttt
    36901 caaaagccag atacgtcgcc tgccagtgcg ttcccgtgag aatgaccggt cgcagcggag
    36961 aggacggggc tgccgatgtc tgggccgtgc tgcaggagga cagcagcaaa accgctaaaa
    37021 acaaaaatat caaagagttc cgttttttgc ttttcaatcg ctttccttcg cctgacggct
    37081 tcaattattt tttctcatgc aagatttcct gtatggttcg aaagcctttg aaatcgttgg
    37141 atgcccaaac atttaaatcc tgcaccgtaa cattactgga gcaaacaaat tgtctggcgt
    37201 caggttccag ttttgacttg ctctgcttct tcttgaaaat tttcaatgca ccgtctttaa
    37261 agagcacaat gtagaattcc atttttgcct ctcaagaata ataaaatcct atcatgagta
    37321 atccttcttt cgccgaacgc ttgaattagc gggctctcca ctgcgcaaag cgaagtggag
    37381 agcccgctgg aatacttcgt tagacatcct tattcagagt aaggcggctc cccttggacg
    37441 gaatttgcca attccatggc cttttttact tgatcccggg ttcccctgaa aacaagccat
    37501 acgcctcctt ccgcgccgct gacgccgccc gcgctgcact ggaaggcggt cactcccgtc
    37561 aggattttaa tggcttcgag ctctgtgacg atttcgcctg taatcagcca ctgggaggga
    37621 ataaggtgac cgggaaaccg attcgtcgac ttaccggatg caggaggcag caattgcacc
    37681 ggctcccgcg ttttcagcgt cagctccgca acatcgcctg caaccacctt ctcgaggccg
    37741 accggaatca cgaggtgaac cttgcgcgcc acgatcatgg gcatggtgat tcccgtcgtt
    37801 cccccttccg ggtgcagaat attaaccgcc gccatcttgt tcctgtagtc gagggcattg
    37861 gcgcccttga tgaccacgtc gcccggcttc agtttcttcg ccgcttcgcc aagcggaata
    37921 tccacgatct tgcctttttc cagaatcacg tggttgacgg ctttcgcctc gggcaggttt
    37981 ttggttcctt tctcgggttc aattcttccc gtcacaaagg ctgcgtgatc ggctttcttt
    38041 ccggtgatct cttccgccac ataggcgttg gttgtcccct tgatgataat gaccataccg
    38101 ttggccagag cattcttgac gatcggcatc tgcgccacag ccttgccgat cagcctcttg
    38161 gattcggcca ccgtcagaat cgcgccgctc tgaaaaattt tttcctcgct ttccggtccc
    38221 gctgcggtag aaattccggc aaacagcaac ggtccgcaaa cggccatcgc caatagcaag
    38281 gcaatccctc tccatccttt tttcatgagg tgttcctcct ttcgctttag attgattttg
    38341 acttattttt taaactaaaa cttcacaacc cgctggggac actgcaatcg gaagcttaaa
    38401 aaaggttaaa gccaggttca agaacttttc gataaaaatc ggaatgggag aataaaactg
    38461 gcggagagag cgggatttga acccgcggta cacctttaaa gggcgtacaa tcgcttagca
    38521 ggcgatcgct ttcggccact cagccatctc tccgctgatt ttttcaggcc tgtatgttta
    38581 aaacagtacg tctcaatctg caagaagttc tgtatgttct ggcggaggga gcaggattcg
    38641 aacccgcgaa cctttcggtc aacggttttc aagaccgccg ccatagacca ctcggccatc
    38701 cctccatcgt aatgtttttc ccggttatcc ttccggcctt acggagtgat ccgctcgagg
    38761 cctctcatgt aaggacgcag cgcctccggg atgatgacgc ttccgtcggc ctgctgatag
    38821 ttctccagaa ccgcgaccac ggtgcggccc acggccagtc cggagccgtt cagggtgtgg
    38881 acgaattcca ccttgccgct gtctttccgg cggaagcgga tcgccgcacg cctggcctgg
    38941 aaatcctcaa agttgctgca tgaggaaatc tcccggtacg tgtcctgtcc gggcatccag
    39001 gcttccacgt cgtacgtttt ggatgaagag aagcccagat cggccgtgca caggcaaacg
    39061 gtgcgataag ggatgccgag ccttttcagg acttcttccg cgttggcggt cagcttttcc
    39121 agctcgtcgt aggatgcctc cggtttggaa aacttgacca tctccacctt gttgaactgg
    39181 tgctgtcgga tcaatccccg tgtatctttg ccgtaggaac cggcttccgc gcggaagcag
    39241 gctgaataag ccacaagata aacaggcagc ttgtcttcct caagaatttc atcccggtag
    39301 atattcgtca ccgggacttc cgccgtcgga atcaggtaat agtccatgcc ttcaatttta
    39361 aaaaggtctt ccttgaactt gggaagctgg ccggtcccgg tcatgctttc ggcgttgacc
    39421 atgaaaggcg taaacacctc cgtgtagccg tggtttgccg tgtgcagatc cagcatgaag
    39481 ttggtgatcg cgcgctccag ttgggcgccc gctccccggt agagtgtgaa tctcgctccg
    39541 gtgattttcg cgccgcgccc gaaatccaga atgttcaggc tttcacccag ttcaaagtgc
    39601 tgcttcgggg caaaacagaa agacggcttt tcgccccagg tccgcacgac cgcattgtct
    39661 tcggaactgc cgccctgcgg caccgattcg tgctgaacgt tcggcacgat catgacaaag
    39721 gcattgagct cctcttccgt gacgcgcagg gtttcgtcat attccttgat tttggccgag
    39781 acgtcaccca ttttggcaat cagtgcggaa gcgtcctctt tcttcttttt cagctcgccg
    39841 acctgcctgg acacactgtt gcgctcattg cgcagggttt ccaccgcctg caaaatgtcc
    39901 cgccttttcg cgtccaggcc gataaaacgg tcaaagtcaa tgttttgccc ccgttcgttc
    39961 atttttcggc gaacggcctc caggttctgc ctcaagtact taatatccaa cataatttac
    40021 cttcagtgct ttggctgacg aatcccgcca aagacgcgtt tccctatcac tttgaacaat
    40081 tgaagtcaat ggttttatcg ggtgaatcaa ttcccggatc cggtcagcct cttttgaatc
    40141 atcttcaaaa aggctctcat ttccgggctt ttgagaaggt aggcgcacgc aaagtatgcg
    40201 actgccccgg tcagaacagc aaggacgaga tagatcagac gttcccgaaa gcccgaggac
    40261 gtgttccagg gcattccata ttccacgagt ccgatggccg ccagcatcgc ggcggatgaa
    40321 gcgatgatct tgaaaacgga ggtgtaaaac gcacggtcga gatacggtcc gattttgttt
    40381 ctcagcacga acgcaaggac tccgacattg acggccgcgg aaatggagcc ggccagcgcg
    40441 atgccgttgt gccggagcgg atacatcagc gtcaggctga aaaccacgtt gacgaccagc
    40501 gtaatgatgc ccgctttcag aggccatttg gaatccttca gggaatagaa ggcggaaacg
    40561 aagacgcgca ggaccgagta ggcccagagc cccagcgcgt agcagaacag cgcctggccg
    40621 gtatagacgg ctgcctgtgc gtcaaacgcg ccgcgctgga acagcacgga gatgatgggc
    40681 aaatggagcg ccatcagggc ggccatcgcc gggatggtca gaaaaagcag cagccgcagg
    40741 gaaaaggaaa tgctggattt cagctcttcg gtttttcccg cggcggcatg agcggaaaaa
    40801 ctgggcagcg ccgccgttcc gatggcaatg gcgaaaatcc ccagcgggaa ctccatgatc
    40861 cggtcggcgt aaaacagata cgtgaccgag ccgctgggca ggagggaggc cagcaccgtt
    40921 cccacaaaga cattgattgt gcccacgccc gcgccgagga tggccggaag catcagcgcg
    40981 ccgatctgcc ggatgcccgg gtggcgcaga ttgaaccgga aatgaaacct tacgccgaat
    41041 ttcccgaggg ccggccactg catggcaagc tgcaggacgc cgcccgccag aacgcccgcg
    41101 gccagcgccg tgatcggctg gtcaaatttg tcgtgcagag cgacggcggc cgtgatcatg
    41161 gcgatgttga gcatcaccgg agacagagcc ggagaggtga aatgccggaa agaattcaga
    41221 atccccatgc acagggcgac cagtgcaatg aaaaagatgt aggggaacat caggcggttc
    41281 agaaaaaccg caagctggta ttggtcggga ttggccatga aacccggggc gatcagtccg
    41341 acaatcacgg gcgagagcgc aatgcccagc gcggaaacgg cggccaggat gacggaaagg
    41401 atcgtaaacg cgttgtcggc cagctccagc gcttccggtt ttgttttttt ctgcaggtat
    41461 tcggtaaaca ccggaacaaa tgaaatcgta agcgagcctt cgcccagcag gcgcctcagc
    41521 atattgggaa tgcgaaacgc cacccagaag gcgtccgtca tccagctcgc accgaaaaag
    41581 gcggcgatga ccatgtcgcg gaccacgccg agaacgcggc tgaccatcgt cgctgcgccc
    41641 acgattccgg cggccttggc gacttttgca ttttctgatt tttccgtagg atcgtccatt
    41701 tcttcaattg cgctttgctt attgccggcg actatgcgtc aggagggaaa tccaggcttc
    41761 ggaatctttg ctccagttcc gttcgattga tgtcgctgac caggattgtc ttcttgcgtc
    41821 ccttggcccc cgcggaaatt ttcaacaggc ttttcctgag ccccagtttt ttggccagca
    41881 gttcgatgca ggcgtcgttg gccgcgcctt ccaccggcgg cgcggtgacc ttaactttca
    41941 gcatgccgcc ggaaatgccg gcgatcgcag cccgcgaggc gtgcggattg acgtggatgt
    42001 caaaggataa gccgttcttt gtttcccgaa tgaggagcgt atcggtcaaa gcgtttgcct
    42061 ttctaaaaac ttcgcgccgc ctggatcagt gtctggacca ggaaatactg cagaaagatg
    42121 atgatcagca aaacgatcag cggcgaaaaa tcaatcggca ggctgtgccg gggaagcatc
    42181 cttcgaatcg gtcccagcac cggttcggtg acgcggtaca ggaaaatgac gatcttgttg
    42241 taaggatcgg gattcaccca ggaaatgaga gcgcgggcga cgatgatcca cagatagacc
    42301 gtcagaacaa tgtcgatgac ttttgccagc gcgatgatga aattgctcaa tgcaaacata
    42361 acttcctcac tgatggatct tttgcttccc gcagggagtt tgtgtcaaag agtcgggcct
    42421 tccgtgaaag cttttctgcc ggcgattcct gtgaagaatc catcagccgg catccggacg
    42481 acgccgctca gggacgggga tccgaaacaa tttcccggca aaaagtgatc agcgcgtcat
    42541 tgaacgcctc gggccgctcc agcatcgcca tgtgtcccgc gccttcaatc aaaacaagtt
    42601 tcgctccggc aattccggcg gcgatccgct ccgacgaggc gggcggcgtc attttatctt
    42661 ccttgccgca gacggccagt accggcacgc ggatcttttc cagttctcca gtcagatcaa
    42721 acttgctgca cgccagcatg tcgcccgcca ctacatcaac actggtctgg cccagacttt
    42781 cccgcagcgg cccaaagagc ttcgggcggt tttctttggc aatggagaat ttgcacatca
    42841 aatccaggat caaatccggc tgtttggcaa acccgtccag aatgtcgggg ttgaccggca
    42901 tggtcaggcc gccgcccgcc gccacgacgg cgcttgcatc ctgcgggtgt tgtgaggcaa
    42961 aacccagggc aatagccgca cccagcgagt gtccgaccaa aaccgggcgc ttcaatttta
    43021 aaacttcgag gatttccttt aaacgcagga cataggccaa aatatccttc tctccgcttc
    43081 cctccgattg cccgtgcccc ggaaggtcga cggccgcgat gttgaacgat ttgtgcagga
    43141 ccgaatactg ttcggaccag gcgccggagt tgccgccgga gccgtgaaga aagaccaggc
    43201 cctgcgcgtg ggcgccgaaa tcagccgggt tgatccagca ggcaatgttg ttgtccccga
    43261 ctttgaaatg tttcagcatg agtgttccct cccgagcccg tgactaccac aaaagcgggt
    43321 ttttatacaa tcttcaatat tgtattgaca ggatgccggg aaaaagggca tgtattggcc
    43381 atccggagga cataaccatg tttacaggca agaaagttgc gattctcggc ggaggcaaaa
    43441 tgggcggcat tctggcgcgg gggatgatta cccggaagct ccttgccccg cagaatatca
    43501 ccgtgacgga cattgatcca cagaggctgg aagagttgcg ttctgcgctg aagctgcggg
    43561 tcagcgatga caaccgtcag gccgtgaagg gggccgatat tattgttctg gccgtcaagc
    43621 cgcagaattt tcccgacctc ctcaaagaga ttcggcaggc tgcgaattcc tcaaagctgt
    43681 ttatttccat cgccgccggc gtgacgacgg atgcgattga aaaatggttg accaaaaaac
    43741 cgcgcgtttt gcgcgtgatg cccaacgtcg ccgcgctggc gggcgaaggc gccgcggccg
    43801 tggcgaaggg gcatttttcc cgccccgatg acgtacagta cgccctggcg atgctgggcg
    43861 ccgtcggcct ggccgtggaa gtggaggaga agctcatgga tgccgtgacg ggactcagcg
    43921 gcagcggccc ggcctattgt tttgtaatga tcgaagccct ggcggatgcc ggcgttcaac
    43981 tggggctggg ccgtgatctg gccgaaaagc ttgccgcgca gacgatgctg ggctcggccc
    44041 gcctgtgtct tcagggaagt catcaccccg cccagttgaa aaatatggtg acctctcccg
    44101 gcggaaccac tgctgccggt ttgcgggtgc tggaggaggg gaaaattcgc gcgacgctga
    44161 cgggcgccgt ggaagcggcc gcgaagaaag cgaaagaact tgccggttca tgaactaaaa
    44221 agccgtgggg gaaaaatgga gatcaggggg aaaaccatca tcgtgacggg cgcggcttcc
    44281 ggcatcggcc ggggcctgtg ccggcggttt gcaaaagaag gcgcgaaggc catcgtcgcc
    44341 gccgacatca atgaagcggg cgcaaaggcc gtcgccgggg aagcgggcgg agaggctttt
    44401 gcctgcgacg tacgcaagga agcggacatc atccgcttgg taaaattcac ggaagaccgc
    44461 tggggcggcg ttgacctttt ctgctccaac gccggcatca tcgcgatcgg cggctatgaa
    44521 gtggacaacg aaacctggca gcgcatctgg gagattaatg ttctcgcgca tgttttcgtg
    44581 gcgcgggcgg tcattcccgg aatgatccgg cgaggcggcg gcgcgatcat gatcaccgct
    44641 tccgcggccg ggctgttgag tcagatcgga tctctgccgt attccgtcac caagcacgcg
    44701 gcggtgggca tggcggaaaa tctggcgatc acctacggcg gccagggcat tcaggtgttt
    44761 gcgctttgtc cgcaggcggt cgagtcggaa atgacgcgtg ggcatgaagg cggcggcgtg
    44821 gcgggggtgg acggcctgat gaagccggag cagctcgcgg acgccgtcat ggaatcgttt
    44881 gccgccgatg aatttctgat tctgccgcac aaggaagtca aaacctacat ccagcgcaag
    44941 gcggcggatt acaaccgttg gattcagggc atgcgcaggc tgcaggacag gttcctgacg
    45001 ggcgcgccgt taaagaaagt ctaaaaaaag cgtttccggc atttttgcat cagcagaggg
    45061 agggaagccc gtggacgtcg aaagatgcgc gtcggtggac aaccttcagg atctggatca
    45121 ggcactcctg gcggacgaca gagtgatcgc catggtgtac gccacctggt gccctttctg
    45181 ccgaaaggtc ctgccggtgt ttgaaaaatg ggcgctaaag gaaaagcggc gcctgcttct
    45241 ggtggccgac gacgacgagc gcgccgcgga cgtgtacgac atcgatattt tcccgacgct
    45301 gatcctgttt gaaaagggcc gcatcgtcaa gcggctcgac ggaaggccgg gcgtcggcct
    45361 cagtgagaaa cagatcgccg atttcatcaa atcctgtccg ccggccgagc ccggctgaaa
    45421 ttcggagtcc gacacatttg gatatttttg ttcgatactg cggcggctgc aactgtcaac
    45481 tggaccggtc caaactggtg agcgacgttc agcgtgggct tggccctgag ttccacctga
    45541 cgacggatct ttccggcgcg ccttgccccg ccgggctgct ggtatgcggc tgtccggccg
    45601 cctgcgtctg gaattccgaa gtcagcgcgg cggccaggcg ctgggtaatc gttgccggaa
    45661 aaacggtgaa tgccgttgaa ataacggagg accgtctggc ggaagcggtg gccgtggaaa
    45721 tcaaatccat tctcttttct gagtaaaaat aaaaa
//
LOCUS       DAOPAF010000006        45419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_954_length_45419_cov_59.212261, whole genome shotgun sequence.
ACCESSION   DAOPAF010000006 DAOPAF010000000
VERSION     DAOPAF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 45419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45419
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_45419_cov_59.212261"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLQ77_01350"
     CDS             <1..611
                     /locus_tag="PLQ77_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL09358.1"
                     /translation="DFQSLINYLLPDVDIRATLIPFYAVSTDLITGKQVVLHEGSLRQ
                     AVLASSSVPGAVEPTRLGEWLLADGGVTSLVPVHAARASGADIVIAVVVDRDLPQEAD
                     IETAKDVLYRAGEITANALEAAELQNADVIIRPAVGDLHWADFSRSHTLIRIGEEAAR
                     ESLEKIHASLPLPRRFARFARRFLNRVQNHDARHTGSNPDPS"
     gene            608..1594
                     /locus_tag="PLQ77_01355"
     CDS             608..1594
                     /locus_tag="PLQ77_01355"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HPL09359.1"
                     /translation="MRLPGQIHSCLLASIAFVLLFTVMCGTVFAAGIEDRQAAVRPWS
                     VSLQTRYFFQSRTSYEFGNPFPPHQAPLSRLEFPLDSWWAGASMRREFSRFSAGVEFF
                     RNLSDEARGVMMDSDWDDAAQPALKTIYSESSCRMEPSYRVRADADLKISDWIGLPGW
                     LDLRPLAGLAWQRFTLVTHDGFQSEIGKGTELLPGDGIRFEQTYWQFFTGLKTTFELG
                     KPFHLTRLAFTMQGDWAYVAGSNEDHHLLRTGNRITREKTTGDAWHALVNLKIGFTQN
                     LSANIGAEYLRIKSTGSHNLTNNIFDIDFTFDHGVKVWSEQWSVATGLEYRF"
     gene            complement(1681..2022)
                     /locus_tag="PLQ77_01360"
     CDS             complement(1681..2022)
                     /locus_tag="PLQ77_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPL09360.1"
                     /translation="MKKQIESERLLLGRRIRSLRNIKGWTQEELGRQADLSYKFIGEI
                     ERGQQNPSLDTIVKIAAAMKVEPLELFRFEQETIDKTVVENRIGRIVKGLPDEDVRRL
                     LMVLRVLYPVV"
     gene            2381..3040
                     /locus_tag="PLQ77_01365"
     CDS             2381..3040
                     /locus_tag="PLQ77_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HPL09361.1"
                     /translation="MKKSIFLLSILVAMMFAVEVYAEAPKFGIGARAGYNSYRNGSME
                     VSVAGFGVAGNVDYSNNGAWTFGFNGILQINKYISAELGLDFINSSKNEFTVAGTSWK
                     AGEIKQMPITLTAQFHYPVGIFSPYVGLGLGYYIRSYDKDNTFFNPAADVSVDNGLGY
                     HANAGSDIFLTKDRNLALNVDLKYVWAKTDITAAAGAAKLVGSMNLDSFLVNFGIKYF
                     F"
     gene            3240..4700
                     /locus_tag="PLQ77_01370"
     CDS             3240..4700
                     /locus_tag="PLQ77_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPL09362.1"
                     /translation="MKTKIIFACAFLLLISGCAAKTPLIKASAEGDTFATEKLVREGA
                     NIDEADDNGYTPLMHAIWSGKQETVAQLVNIGADVNKRDKTGYTPLLWAASYGYLDMA
                     KLLIDKGADVNARGNDQSSPLLLALAANNHELSKLLIDRGVDIHVVDVNGTTPLILAA
                     MYCDYDIAKIFIERDVDLFAVDASGYKASDYVKFSLKTGKRVFQSDDYERNLSFLKLI
                     KKAENAQILAGRMNTKNPKMARIYSIVYKVGKCINPDVDYDVFISDKNEPNAWVNISG
                     NITFTKEALEKFDNDTLTFVAAHEIAHDKLGHVAKKIAVSSTITGALVVANVFLPGAG
                     YLNHIINPTITNNYSKTQEYDADQMASDHCAQCFDMSIEKQITVMQKIEKISKSSGGG
                     FWATHPSWENRIKNVATTTKSSSQIRNIKTTAQSPSPLMEGDQKHKEIKGIVLNDGNV
                     VEGQLLEMSTDTVMIQTEDGKVSTYSFEKEVRGFVK"
     gene            complement(4769..5062)
                     /locus_tag="PLQ77_01375"
     CDS             complement(4769..5062)
                     /locus_tag="PLQ77_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09363.1"
                     /translation="MKAFSVMPTEAGIDLILISEDFRNHDYWSRIDILSEAIYDVFVP
                     IEVVGFTPEEWDNQTSPLYDYAKQGEILYAARPCQCYDAFPCFSLRKFISEPV"
     gene            complement(5115..6803)
                     /locus_tag="PLQ77_01380"
     CDS             complement(5115..6803)
                     /locus_tag="PLQ77_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL09364.1"
                     /translation="MNWKEKKKYDALLARERGVMKKVWGTCLTVCLAYPNLYRIGMAN
                     LGFQTVYKAINDLPSLLCERVFLPAGDGAEYAAGAVEPLSLESRKPLRDFDILAFSVS
                     FENDYPAILKMLEWSGIPLKSKDRADGHPLVIGGGIALTLNPEPLADFFDIFVLGEAE
                     EILPQFARVFAEARRLELDRKDLFNDLQKQIPGIYVPSLYAVSYFPEGKIKDIAPRTE
                     GLPKKIQISPIRNINAFCTTEVVSGADAEMADMFLVEVNRGCPHLCRFCAAGFVYAPP
                     RFRGYEEIVAAVDRGLMIKNKIGLVGTAVSDHPDLIKICRYIVGRGAQAGIGSLRLDK
                     IDESIVDLLKTGGIETVALAPEAGSQRLRDLLRKGITTDDILNAAKLLIEKEMSNLRL
                     YFMVGLPSEEDSDIDAIIDLVKKIQHTALAQTKGKRKFRRITLSINQFIPKPRTPLQW
                     CPLAEVQDVGRRIKKITHAFRSDKQIHVIADVPKWAYVQALLSSGDRRVGDILLAAHR
                     LNGNWMRAFKEVTINPDFYVYRQKDVNEFLPWDIIEAGLPKKALIKEYQKALPD"
     gene            complement(6862..8010)
                     /gene="ftsZ"
                     /locus_tag="PLQ77_01385"
     CDS             complement(6862..8010)
                     /gene="ftsZ"
                     /locus_tag="PLQ77_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPL09365.1"
                     /translation="MFELTEECIENSARIKVVGAGGGGGNAVNTMISYTLRGVDFIIA
                     NTDAQALGASSAPVKIQIGAEVTKGLGAGSNPDIGKQAALESRDEIRRHLEGADMIFI
                     TAGLGGGTGTGAAPVIAEVARECGALTVAVVTKPFQFEGKRRNAQAEDGIVQLRDVVD
                     TLIVVPNQRLLSLGGRGLSLLDAFKKADDILYQAVKGISDLIIVPGLINLDFADVKNI
                     MSNMGMALMGTGTASGENRAVEAAQRAISSPLLEDNTIQGAQGILLSITGGPDMSLYE
                     VNEASSLIQAEAHEDANIIFGTVIDENMKDEIRITVIATGFEDAKKKQVPSNVTHLGG
                     FRREDLSTPAFMRKSKSGDPANVVTMGVNDENENVDIDIPTFLRRQAD"
     gene            complement(8029..9255)
                     /gene="ftsA"
                     /locus_tag="PLQ77_01390"
     CDS             complement(8029..9255)
                     /gene="ftsA"
                     /locus_tag="PLQ77_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPL09366.1"
                     /translation="MAKKSNVLVGIDIGTTKTIAIVGEVKTTGIDIIGMGITPAKELR
                     KGGVVNIDSTVEAIKKAVEDAEHMSGCRINSAYVGIAGSHVKGQNSLGIVAVKGREVG
                     EDDLQRALEASKAIAIPVDREILHTLPQNYVVDGQDGIRDPVGMSGVRLEAKVHIVTG
                     AAASIQNVVKSVNRVGLDIEDVVLEQLAASEAILSADEKDLGVALIDIGGATTGMAIF
                     AEGSIKHTATLPVGGNFLTSDIATGLRTPFAEAEKIKLNHGCAMTSMIPKEDVIEVPS
                     VGGREARMVSRQILGRIVEPRMDEILNMALKEIVRSGYEDLLAAGIVLTGGSSLLPGI
                     NEMAEQIFDMPVRLGCPAGIGGLSDVANSPAFAVGVGLIVYGSKNIPGDSVYRKTGNA
                     FGEYFKTLKKWFLEFF"
     gene            complement(9280..10119)
                     /locus_tag="PLQ77_01395"
     CDS             complement(9280..10119)
                     /locus_tag="PLQ77_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPL09367.1"
                     /translation="MSQKIKVNLEAKKNRLRRRLIGALNESLGALALLAMAVAASAVL
                     IYLYNALLSAPGLELKEISVRGVKELTEKDILELAKIQPRCNLLAVNTDAVARRITAN
                     PWVRKIYVGRELPGRLVLDVRERTPIALVKQTGNFYLMDAEGVVFKKLSRTDDVDLAI
                     LTGVNLGKAPSPLLAEALKLLEILSADSYASLGTISEIHIDEVFGLSVLTDRGLHLKL
                     GRDNFTGKVRQLDVVLADLEKRGMKNGRLFVDLADISKVTVQRKDAPGKAQEQKKGPQ
                     YRI"
     gene            complement(10116..11042)
                     /gene="murB"
                     /locus_tag="PLQ77_01400"
     CDS             complement(10116..11042)
                     /gene="murB"
                     /locus_tag="PLQ77_01400"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPL09368.1"
                     /translation="MTGHLKISAALQGANVGKVWFDEPISRHASLKLGGRVDALVMPE
                     SEVQLAELVRRLKENHLPFLPVGNLTNILVRDGGYRGALISLRGLDRAVCAPAEDGRF
                     RIEAQAGAALGRIVGLAADHELTGLEFCAGIPGSVGGAVWMNAGAFGAEIKDVLVFAD
                     LIDDGGERKTLRREEIAFSYRKSNLPANVIICGARFALSKGRSEQIRDRVAEIMKWRQ
                     EKHPLQYPSAGSVFKNLPGMPAGRLLEELGLKGRRLGDVQISKKHANFMINKGQGTAS
                     DMVKLIRLVQEMVQREKGLFLETEIVVIGEEW"
     gene            complement(11039..12451)
                     /gene="murC"
                     /locus_tag="PLQ77_01405"
     CDS             complement(11039..12451)
                     /gene="murC"
                     /locus_tag="PLQ77_01405"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPL09369.1"
                     /translation="MSKDQETGFMQRKVKHIHFVGIGGIGMSGIAEVLLNLGYGISGS
                     DVQSSDTTKRLQQLGAAVAVGHTAENIGGADVVVTSTAVKADNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTLLAEGGLDPTMVIGGKLASIGSNARL
                     GGGDILVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIEEIKDAFLKFANMVPFY
                     GCTVMCVDNEHVRAIARSIKRRVISYGISQPADYTASDIRHSDAGTRYALSYRGEPLG
                     DVALMVPGLFNVYNSMAAIAVGRELGLEMATLCKGLLAFSGVQRRLEVKGEKAGITVV
                     DDYGHHPTEIMATLAAARQMRRGRLIVVFQPHRFTRTKALFDEFTRAFGDADVLVLND
                     IYPASETPIPGVHSAALWVAIKEGGHPCVEYIAQPQGTIEFLLANAKPGDTVLTLGAG
                     SVYKIGEAFLARLKEGTKNP"
     gene            complement(12448..13554)
                     /gene="murG"
                     /locus_tag="PLQ77_01410"
     CDS             complement(12448..13554)
                     /gene="murG"
                     /locus_tag="PLQ77_01410"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPL09370.1"
                     /translation="MRIAIAGGGTGGHLFPGIAVAEEFLKRDPQNRVIFIGTRRGIEH
                     RLLGTLGYELKLIDVEGLKGRGLKALIKGVYAIPNSMIQSGRILAEFRPDAVLGVGGY
                     ASGPAVLAARLMGLPTAIAEQNALAGITNRILGKFVDQIFLTYEQSRGRFDVRKVRVT
                     GNPVRAAFVAGLVVAPTSRAFRQILVFGGSQGAAAINKTVVAMLPMLQGMRRRVRVVH
                     QTGERDLAAVRQAYGKAGVEADVQPFIVDMASAYASADLIICRAGATSLAEITAAGKA
                     SVLIPFPYAANDHQTENARAMVRAGAAAMIPESGLTAGTLYLLVETLLEDGEKLRGME
                     EKSKALGRPGAAAEIVDACLRLGAKQKNTQERGT"
     gene            complement(13596..14702)
                     /gene="ftsW"
                     /locus_tag="PLQ77_01415"
     CDS             complement(13596..14702)
                     /gene="ftsW"
                     /locus_tag="PLQ77_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPL09371.1"
                     /translation="MDAAKKDANTPDVILLLVTLILVTVGTAMIYSSSSILAMEKFKD
                     GQFFLKKHLFFVFAGLVIMIVMTKIPYEQLKKLAYPGVIVSVVLLLLLFIPHVGVKRG
                     GATRWLNLGVFSFQVTELVKVAMVLFLAHLLTRKVHHLKKFSRGVLIPLSVTAVVMGL
                     IILQPDFGTVVIMMSILLLMLSLAGSRITHLMFLGAAFIPVGIWLIMSKGYRMARLTA
                     FLDPWKDADKTGFQLIQSLISFGSGGVTGQGIGDGMQKLFYLPEPHTDFILAVIAEES
                     GFIGVALIIVLFIFFMLRGFMIAFRAPNLFGTLLAAGLTMLIALQAFINIAGVMGLIP
                     LKGLALPFLSYGGSSFVMSMMTVGILLNISTQRT"
     gene            complement(14703..16046)
                     /gene="murD"
                     /locus_tag="PLQ77_01420"
     CDS             complement(14703..16046)
                     /gene="murD"
                     /locus_tag="PLQ77_01420"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPL09372.1"
                     /translation="MQLADRKIAVIGMGKTGIAAAAFLGGSGAAVVAVDEKPRDQWGG
                     EFEQIAHRPWLAVGRYDETCLDGVDLVVPSPGIPPRHPLLTAAQQKKIPILSEVELAC
                     RFIRVPIIAVTGTNGKTTTTTLLGEILARAGKKVFVGGNIGNPLIEYAGAAQTDDFIV
                     AEISSFQLQWIESFRPHAAILLNITCDHVNYHGSFEEYRRIKGRIFENQRENDLAVLN
                     AEDPQQEGLDASMRACVVRFSSVRPLAPGLCLQKDSLVYSGPDGTDERYPLGMIRIPG
                     LHNVENVMAAVAVARFCGCSKESIVEAVSQFRGLPHRIEFAGEKRAVQFYDDSKGTNV
                     DAVVRALQTFSSPVILLLGGRDKDGNFDVLQPMLGAKTKQVVLFGEARDQIEPQLGNT
                     TPIAKEATLKAAVLRAYRDAKPGDIVLLSPGCASFDAFANYRERGNHFKEVVRNL"
     gene            complement(16054..17133)
                     /gene="mraY"
                     /locus_tag="PLQ77_01425"
     CDS             complement(16054..17133)
                     /gene="mraY"
                     /locus_tag="PLQ77_01425"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPL09373.1"
                     /translation="MIYEFLYPFHTVFSSFNVFRYITFRTIFASITALLICLVFGKWF
                     IDKLQRMQIDQQIREDGPKTHLVKKGTPTMGGMLIIFAVTVSTILWTNLSVVYVWLVL
                     LVTVGFGLIGFLDDYRKLAGKNSRGLSGKTRLTAEMIIALFVGVVLYFKPGFNAQVTI
                     PFFKTVLPNLGWGYILLCVFIIVGAANAVNLTDGLDGLATGPAGICFATYLLFAYFAG
                     NIKVAHYLQIPYVPGAGELSVFCGAFMGAALGFLWFNAHPAEIFMGDVGSLSMGGALG
                     AVALITKQEILLAIVGGVFVMETFSVIFQVGYFKLTGGKRIFRMAPIHHHFELKGWAE
                     PKVIVRFWIISILLALLAISTLKLR"
     gene            complement(17149..20115)
                     /locus_tag="PLQ77_01430"
     CDS             complement(17149..20115)
                     /locus_tag="PLQ77_01430"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPL09374.1"
                     /translation="MMELRQLLQGIQTLQTETDPKGDVSSICYVAEACGPGSLFVAIP
                     GLAFDGHHFIGRAIERGAHFIVHQKDIVVPDGVKAIRVPDSRRALGTLAKNYFGDPSS
                     RLTLIGITGTSGKTTVSYLLESILTAAGFSCGVLGTINYRYGGKQFPAPNTTPESFDM
                     QRILSEMACAGVTHVIAEVSSHALDLKRVDDCDFDLAVFTNLSPEHLDYHKDMEDYFR
                     AKKRLFAELLPRSRKNLARKAVINADDPRGRQLIREISVPALSYSLEQDGAARVQNPH
                     VTLGGVQADILLDGEKISVTSGLIGGFNLSNILAAAAAASVLAVEPRAIARGIQNLSL
                     VPGRLEKVVPSGGVHVFVDYAHKPDALEQVLRNLHGLKEKRILTVFGCGGNRDRAKRP
                     VMGDIATTWSDLTIITSDNPRREDPLEIIREIEAGIDGGKIRKVPQDRLEFDAGAHGY
                     AILADRRAAIEAAVRIAEPQDIVLIAGKGHEDYQILGTKKVPFDDRLAAMRALRLKFP
                     GWMSPAFSLAEVLQATGGRLAAGERDQAVYGVSTDSRQIHPGNLFVALAGENFDGHAF
                     VPKAVEGGALCVLVADADRIDLKALTRRAAVIETADTLRALGDLAHMHRRRFSIPVIA
                     LTGSSGKTTTREMLTCILRQEKTVLGTEGNLNNLIGLPQTLFRMTDRHDAAVLEMGTN
                     TRGEIKRLTQIAAPDIGLVTNVGPAHLAGFGSVETVREEKGDLWLHMNPAGVAVVNLD
                     DQAVRQAAERWSGRRVTFSMSAGADVGVNDIRKNGARGVSFQLLMNGQAHKVDMKAAG
                     IHNIYNAMAASAAALACGVSVESIRRGLSMFQPVAGRMEILRLQNGAYLINDAYNANP
                     ASVREALLTLRDLASSHNAFVFLGDMLELGDAAQEMHRKIGLLLATIGTAAVFLQGDF
                     ARATAAGALEGGLAEERIVFLCDDGEAVAFLKKKLRKGDWVLIKGSRRMKMDRLAARL
                     GEEIGVEKAEDHAAAT"
     gene            complement(20119..22107)
                     /locus_tag="PLQ77_01435"
     CDS             complement(20119..22107)
                     /locus_tag="PLQ77_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPL09375.1"
                     /translation="MAADSKKWLKFRLASILAIFLILFIALLSRAFQLQVLSGEKLKR
                     LADRQHMTTLSIHAERGMIYDRNGEKLAMSVMAESVCADPSRISNPAKVSREISSILN
                     LDPSSVLKKISEPKSFCWLARKISSQQALMVESANLEGIFLIKEPKRFYPNGPLAAHL
                     IGFSGFDAEGLEGLERKYDRYLKGTPEKLTWARDAKGKKLFVHVEQDPSEKMGSANLV
                     LTIDSRIQYLAETHLKEAVLEKGAKGGVVIVMDPKTGEILAMANEKAFNPNNIKGLTS
                     ENWRNRAITDSFDPGSTFKPFLVAAALEEKVVKESDRFYCENGHYQVANRVIREANHK
                     KHGYLAVRDIIKYSSNIGSAKIAEKLGREKFYAYIKNFGFGSRTGIDLSGEAAGVLRP
                     VKSWTRVDTTNIGFGQGISVTAIQLVTALATIANGGVRMKPYIVKGFTDRNNQPVKMF
                     APETVRRVISPETARRMAVMLTEVVGAEDGTGKRAHIVNVPVAGKTGTAQKFDFARGV
                     YSSEKVRTSFIGFFPSDNPQVVILVMLDEPQRDKWGGVAAAPVFKSIGEQILNCFKTN
                     IRETPAFEPQPVHELQLVSADASLVEDMTAAENEINDESRMPDFKGLTIREALKMARS
                     RSIELRVSGSGWATQQFPQPGSEPGEDRLCKVIFEMKN"
     gene            complement(22110..22457)
                     /gene="ftsL"
                     /locus_tag="PLQ77_01440"
     CDS             complement(22110..22457)
                     /gene="ftsL"
                     /locus_tag="PLQ77_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPL09376.1"
                     /translation="MAQTAGTETLTQGRPLKEADAASFGVKYPAFIVVALVIMFVAVI
                     YVGSHIRMTQMEYDVAAALHTKEQLMEEQKKLKLELAMLKSPQRIEDIARTQLQMTYP
                     TAAQVIVLKQTRK"
     gene            complement(22459..23394)
                     /gene="rsmH"
                     /locus_tag="PLQ77_01445"
     CDS             complement(22459..23394)
                     /gene="rsmH"
                     /locus_tag="PLQ77_01445"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPL09377.1"
                     /translation="MSLVFSHEPVMREEVLSWLVRNQSGVYVDGTIGGGGHALAILEN
                     TRASLVGIDLDAEAISAARQRLDSFQPRVKLVKGNFADLASVLDGFNIEKVDGVILDL
                     GVSSHQLDAAHRGFSFSQSAPLDMRMDRDAKLRAYDIVNGFKPSELERVIRLYGEEKM
                     AARIAGAIVRRRDVSPIETTAELAQLITQAMPHSMKHQKIHPATRTFQAIRIAVNREL
                     DTLAPGIEAAVAALSTGGRLGVISFHSLEDRIVKHTFRDLAATCVCPKDVPRCVCHKQ
                     AVVKVLTRKAVTPSLEEIRRNPRARSAKLRVAERI"
     gene            complement(23397..23843)
                     /gene="mraZ"
                     /locus_tag="PLQ77_01450"
     CDS             complement(23397..23843)
                     /gene="mraZ"
                     /locus_tag="PLQ77_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPL09378.1"
                     /translation="MSVFRGQYHHTIDEKGRIIFPSRFRELFAEKYDNRMVITNWDGY
                     LIAFPYAEWRLIEEEVSKRRLLDKKFRDFQRFFMSGAVDCNLDSQGRVLVPPTLREYA
                     HLEKDIVLAGMLKNIEIWPRDKFEERNREAGEKIDRDNEIAEQLGI"
     gene            complement(24124..25167)
                     /gene="mltG"
                     /locus_tag="PLQ77_01455"
     CDS             complement(24124..25167)
                     /gene="mltG"
                     /locus_tag="PLQ77_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPL09379.1"
                     /translation="MKSRTKKTLFYFFSLPAIAAVVFGALLFIYAVTPMDSAKDKSGV
                     VDIPTGTSLIRVINILHNAGLVQNRVLFHALVVVKGGARSVRAGEYEFAADISPSVLV
                     DKLLHGEIKQYRVTIFEDSSISQVASRLREYKLINEKDFFELAQDREFLSFLDIQSAS
                     IEGHLFPDTYFLDRSMTTRQIMRIMVERYHSRVSPEMIRKAAQKGLNANQFVTFASLV
                     GKESGYAAEKPMIAAVFYNRLKKGMPLQCDPTAVYDLKNFKGKVKRFHYKRESPYNTY
                     QIRGLPPGPIASPGLDSFQAVLNPAGVDYLYFVSKGDGTHFFSSSLVAHNDATIRFRN
                     MMNREEKTDEVVR"
     gene            complement(25207..25632)
                     /gene="ruvX"
                     /locus_tag="PLQ77_01460"
     CDS             complement(25207..25632)
                     /gene="ruvX"
                     /locus_tag="PLQ77_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPL09380.1"
                     /translation="MRILGVDYGEKRIGLAVSDGLGLTAQGLPTLIRKTKKNDLEILR
                     RRILDYSVERIVIGYPVRIDGSEGIQCEKVNRFAGRLAETFMLPVVKWPETLTTKEAE
                     DILRASGVHWRKRKDKVDRLAACLILQDYLDNAGKREPG"
     gene            25796..27709
                     /gene="selB"
                     /locus_tag="PLQ77_01465"
     CDS             25796..27709
                     /gene="selB"
                     /locus_tag="PLQ77_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPL09381.1"
                     /translation="MQHFVLGTAGHVDHGKTSLIKALTGVDTDRLKEEKERGITIELG
                     FASLSLPSGHTLGIVDVPGHEKFIRNMVAGAAGIDLVLMVIAADEGVMPQTKEHLQIC
                     SLLGITTGMVALTKIDLVEQDWLELVRSEISEYLQGTFLAGAPIVPVSASQGQGIPAL
                     LEEIDKTVRRLGEKADDGIFRLPVDRIFTMKGFGTVVTGTLISDKIKIGEEVQILPEK
                     ISARVRGIQVHNQSTEEAFSGQRTALNLQGIERSTIERGNVLVRPETVWPTQRLDVFF
                     EYLASNARKLKNRSLARLHTGTAEIMVRFVLLDADELSPGDAAFAQLILENEDVVVGG
                     DHFVLRSYSPVTTIGGGRILDPHPGKHKRKNQKILDDLRLLQSGALPEKILVMLDRAG
                     FHGIPIRSLAFRLGASVKKIREALEKLFSDRRAVLLSNEDATALSAGLYAHLEELLIK
                     SLANYHRSHPLKEGISKEELKAALPEAVSGKLFNMVLAGLARKERIASDKDNVRLASH
                     HVQLEGEEDALRQAIASVYAQAGLAPPSLSDVMVRFRDQKARAQNIVRLMIKDGDLVK
                     INEELCFARAAMDKLREDYKGQLIRDGQATPATFKDLTGLSRKYIIPLMEYFDASKLT
                     VRVEDHRILREKP"
     gene            27737..28693
                     /locus_tag="PLQ77_01470"
     CDS             27737..28693
                     /locus_tag="PLQ77_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL09382.1"
                     /translation="MKTKDLYLQDIRPGDKIASSFLVTEKNMAFSQKGSAYLNVRLKD
                     KTGEIDAKVWDNAVALDRVFKKGDIISIEGRAQSYRNAVQISILTAKTCGREDVDPSD
                     YLPASKLDTAAMFEDLRAYVGTMSSEPLKKLLDAFLNDEKTADLFRRAPAAKGFHHIY
                     LGGLLEHTLSVVRLLDRAAEHYPQLNRDLLIAGGILHDIGKIYEFSYDGIIEYSDEGR
                     MIGHLVMGVEMINQKMAAIDDFPARLALELRHIILSHHGEFEFGSPKRPKTREALVIH
                     FMDDLDAKLNGFESFIEGDAANPGSEWTAYNRFFERYLYKGK"
     gene            complement(28918..29892)
                     /locus_tag="PLQ77_01475"
     CDS             complement(28918..29892)
                     /locus_tag="PLQ77_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPL09383.1"
                     /translation="MASIQQRKERVAMLSVASNAVLVIFKVFVGALIGSVSILSEAIH
                     SGMDLLAAVIAMFSVKTSHLPADDVHPFGHGKVESLSGLIEAALIFIAAFWIVFESVR
                     KLTSAQQVESPGWGVAVMLLSAVLNYFVSRRLFAVGKEADSIALTADAWHLRTDVYTS
                     VGVMLSLAVIWVSHFFFADPRIHWLDPVAAIFVAVFILKAAYDLSAKAIGDLMDVKLS
                     PDEERWIRSVIVSHKPDIHGYHQLKTRKAGNFRFIEFHIKVDPQMTVEASHGIARDVK
                     HAILTQYPAATVTVHVEPCDGNCTEICVAGCLLPEDRRRGIARCARSL"
     gene            30038..31126
                     /locus_tag="PLQ77_01480"
     CDS             30038..31126
                     /locus_tag="PLQ77_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPL09384.1"
                     /translation="MRKNFVCRFPDGTSRNWRDWRWQLRHRATSLIALTRILETPPRD
                     GKPLERVMSRYPMAITPYYLSLLEPDHENDPLSLQCVPDLRELSFSAGLRDDPLGEDR
                     SMPAPNLIQRYPDRVLAIVTHRCATYCRHCNRKRFWSVPEAGPLKNRLAPMVRFVSES
                     PQIREVILSGGDPLMFDDASLEWILSSFASIPHVEVLRIGSRAPVVLPMRITGNLCRI
                     LKRHRPLWFNTQFNHPSEITKDAQRACNMIQEAGIPVSNQSVLLQGVNDSPKVMQSLL
                     NGLQKISVRPYYLFHCEPARGCNHFRTDARKGLNLMEKLHRSCSGLALPQYVADLPGR
                     EGKVPLWPLSRPIQNALRKHQDFFDNFE"
     gene            31189..31752
                     /gene="efp"
                     /locus_tag="PLQ77_01485"
     CDS             31189..31752
                     /gene="efp"
                     /locus_tag="PLQ77_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPL09385.1"
                     /translation="MYTASDLRKGLRLKIDNDPYVITEFNFVKPGKGQALYRCKLKNM
                     LTGSQFERTFRSVDVFEPADLREKKMQYLYKEGDKFCFMDNETFEQVYLTEGQVGDAV
                     NFLLDNIEAEILLFEDRPIGISLPNFVELVVTEAEPWAKGDTVSGATKPVKVQTGYTI
                     LVPTFISEGEKIRIDTRTGEYLTRVKG"
     gene            31758..32696
                     /gene="epmA"
                     /locus_tag="PLQ77_01490"
     CDS             31758..32696
                     /gene="epmA"
                     /locus_tag="PLQ77_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HPL09386.1"
                     /translation="MSTLRQEDSFRLARKSQALRLRAEFTQCLRRRFLEQGFLEVETP
                     LRIPSPAPEEHIEALASDGWFLQTSPELCMKRILAAGYPRIFQFCKCFRAGERGGRHL
                     PEFSMLEWYALHTDYQMLMRQCEALLQSALADTGHSEKIIWRNQIIRLPLPWERITVK
                     DAFSRYAPVSLQNALAGGRFDEVLTEHVEPNLGCDHPVFLCDYPASMAALAKLKEGDP
                     AVAERFELYMGGMEIANGFSELTDAQEQRRRFEEALEARAAKDLARYAMPEPFLAALE
                     GLPPCAGIALGMDRIIMILADAPNIDDVVAFPPETL"
     gene            32803..33498
                     /gene="tolQ"
                     /locus_tag="PLQ77_01495"
     CDS             32803..33498
                     /gene="tolQ"
                     /locus_tag="PLQ77_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HPL09387.1"
                     /translation="MESHFHGSLLGMILDAGLMVQFVLLILLIFSIISWTIIFIKFRT
                     YRRIRQENEAFDSDYQRSTKLSDLLPASKKYPFSTTAKVFRVGYTEMTKANRLSRDAA
                     RTEDISLSSLDNVERALNRASSTEMTKLESALGFLATTGSASPFIGLFGTVWGIMETF
                     KSIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNKSRTMVQEMDNFAD
                     EFLNIVERYLVRK"
     gene            33560..33946
                     /gene="tolR"
                     /locus_tag="PLQ77_01500"
     CDS             33560..33946
                     /gene="tolR"
                     /locus_tag="PLQ77_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HPL09388.1"
                     /translation="MAEINVTPLVDVMLVLLIIFMVAAPMLSMGIDVNLPRVKSKSID
                     LAEEKLILSINENREIYLNKTRLPVQDLNAKLAAIFAARIDREVFLRADKNVPYGFVV
                     EVMSEIRKAGVDKLGMITEPPPEGAK"
     gene            34000..34671
                     /locus_tag="PLQ77_01505"
     CDS             34000..34671
                     /locus_tag="PLQ77_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPL09389.1"
                     /translation="MFLASLFLHLVLMAAVFATVPGSSKPLTFGAPYSVSLVGPEVIG
                     PGSQEAPDQKSLLKTISSDSSVILKREIGASPSSFVMKKDDSGRSDIEKAIRALEQKE
                     TAAGKNKNPAAASTPSGTAASQEYASQLNAYSRFVWAKVKRNWSLPATLMPKKNVEAV
                     IEVRIAQSGSLEHIGFEKRSGNSYFDESALRAVQKSTPFPPPGGWASGRFVEIGIRFH
                     SAELR"
     gene            34698..36014
                     /gene="tolB"
                     /locus_tag="PLQ77_01510"
     CDS             34698..36014
                     /gene="tolB"
                     /locus_tag="PLQ77_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPL09390.1"
                     /translation="MDKKYRLALALILVSLWMVSAAGFAAGKVYIDIDAPGFQQFPVA
                     ILDFQNQKAGTGASPNDPADSIRRYLDLTGIFRVLDKKSFLEGIEPGSPEVIRFSDWS
                     AIGADFLLRGNLRDGGNGLSAESRLYDVTRGQVIFEKKYSAPDGSPQTLGLSIASDIL
                     LALTGDAGDFRTRIAFVIKKKSGSDIFTMNYDASDLKNMTNDHSIVAAPRWSPDGRYF
                     AFTSYKNGRPMVFLRHLKTGKEKTIASFSGLNLCGSFSPDGRKILLTLSKDGNEEIYA
                     LDIETMHLNRLTNSFSIDVSPAWSPDGRKIAFVSNRSGSPQIFVMDADGSNVKRVTFE
                     GNYNTSPSWSPRGGRIAYEGLINRKYQIFSADEEGNNLIQLTFDEADNEYPSWSPSGR
                     QIVFHSRQNGKSRICIMNANGMNLRVLKESRDLLVMPAWSPRPGKQ"
     gene            36106..36738
                     /gene="pal"
                     /locus_tag="PLQ77_01515"
     CDS             36106..36738
                     /gene="pal"
                     /locus_tag="PLQ77_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPL09391.1"
                     /translation="MNRHLTVIGLLLIFVFSLTIFSGCAEKQAVVKDEAMQEQKVAAA
                     QEGAPAPVAADDDAALRAKEAADREAALKEQAAKDEAARKAAARAAWAKKNAEALVDL
                     NIQNIYFDYDKSGIRPDAREILKANAEIFTKNSAAKIIVEGHCDERGTAEYNMALGER
                     RAQEAKQYLVNLGIDASRIETISYGKERPLDNRSIDEAWAQNRRAQFLLK"
     gene            36763..37587
                     /gene="ybgF"
                     /locus_tag="PLQ77_01520"
     CDS             36763..37587
                     /gene="ybgF"
                     /locus_tag="PLQ77_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPL09392.1"
                     /translation="MKRFWYLILPAMVFSLAACATSSDLKALRTELNQQMEAKFAAAD
                     TRLDDRVSAVQNAQAKNTEDLAALRKATANTGVNISDLRDQVQQLRGQVETLKKETAR
                     DPKKDAEVQEKIDQLLVKVNFIENFLEIGKKDAAGAGNGATPGKDHGKQGKAAVYAAA
                     YSTFKNGQYTKAREAFQDFLTAHPTGEYSDNAQFWIGECYFFEKNYEKAILEYEKVTK
                     KFPSSNKVPYALLKQGLCFQNLGDKVSARLLLQQVIKDYPNTNQARIARSKLQEIK"
     gene            complement(37656..38438)
                     /locus_tag="PLQ77_01525"
     CDS             complement(37656..38438)
                     /locus_tag="PLQ77_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPL09393.1"
                     /translation="MRHGYAPAAGIMAMLLLSLLACGCAAGLAFTGPASSPDSAERIR
                     RTPGETDRIVATARMDLSATEGHYPLRAALVLQKPSFLRLEALPVIGTPGFFLSATPK
                     EMRIWIPSQNEFYAGSPSAENMARFLPWALNLEDLVMILSGACPRLDAAGVTYRHSEE
                     SGGLRVDMKTPSGPSQTIWMEKSGPLTRLVRYAPDGKEIYSVRYEGYGPSLALPGKIF
                     IQWADREASVRVEYDDLKIEPSADPSVFALPVPDGVKIIRLD"
     gene            complement(38478..40181)
                     /locus_tag="PLQ77_01530"
     CDS             complement(38478..40181)
                     /locus_tag="PLQ77_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL09394.1"
                     /translation="MQKKHIPLLICGLLFWALLAGCASFPARTRPAAPDPAPETYSKE
                     AAYHYSLAVLSRLDGRPAESIDHMKRALSFSPDSPYLNTELVSLYVENGDVEPALALG
                     EATLARQPGNIELRSIMGGLYFNLREYDKAVQEYRTVTETDPDNAVAYLYLATIYSQE
                     KKYAEAEKAYRELLALDPDNIIGMFYYAKTLSQMNRPADAESLYQKIIAQRPAFEAAW
                     TGLAALYESRNRYDEAIGVYRRYLAVHPARIGIRVKIAELQMKANREEAAEKELREVL
                     KISPDNREVIMALGLLYFDMRRYDEAAAEFALLLEKTPADDKLRYLLASVLEQKGDRQ
                     KALTEYQKISPKFELYANAQIQAARLTNLEGDSAGAIGIIRRAIGQKKDQAVLHLYLS
                     SLYEEKKDMAAAEKTIREGISLFPGNTDLHYLLGALLEKTGRLEESIQSMRRVLSLDP
                     GNADALNFIGYSYADRGIHLDEAEQMIVQALKIKPDSGYILDSMGWVHFRKSNYESAL
                     TYLKKALEFLPEDPGVMEHLGDVYFETGRRQEALEWYRKAIKADPANSRLKNKMDLLK
                     P"
     gene            complement(40229..41179)
                     /locus_tag="PLQ77_01535"
     CDS             complement(40229..41179)
                     /locus_tag="PLQ77_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPL09395.1"
                     /translation="MKKKKKIKSAAAVLIVLIVLAVAAIFFMVLSDREETEKVAPPRA
                     AEKRAKPSAAESRAAAKTQPRAAKDAVPPPESGVGTEAAPSRTEGRQVAIIIDDIGQD
                     MKPVRELLDIDANITFAVLPLLPHSREAAETLHRAGREILLHLPMEPLSYPKEKPGSG
                     ALFTDMNNEEILFQMERNMASVPYASGVNNHMGSKFMADEEKLTLVFREIKKRNLFFI
                     DSRTTGNSKTQAAAQRARLRVASRKIFLDNERDYSRIYRILMEAARASDGNSPLIVIG
                     HPYPETIRAVREAGKVFREQGIAIVPVSALLSKSKPEGAS"
     gene            complement(41214..42533)
                     /locus_tag="PLQ77_01540"
     CDS             complement(41214..42533)
                     /locus_tag="PLQ77_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL09396.1"
                     /translation="MKNISGKWPILAVILFGLFVVFGPYSDSKVSARDKNIYKEIKTF
                     NEVLDMVQKNYVEEIDSSVLIQGAINGMIKSLDPHSSFMTPELYKELEVETQGRFGGI
                     GIEITILKDVLTVVSPIEDTPAFKAGVKPGDSIVKIDGKSTKDITIMEAVKKLRGPKD
                     TKVTITILRENMAQPRDITLTRAVIQVKSVRAKAFDDHIGYIRIASFHERTADDLRKA
                     LTDVAEKTKPMKGLVLDLRNDPGGLLTQAIEVSDMFLKSGVIVSTRGRTKKMETKNTA
                     RDSGDKEVVVPMVVLVNEGTASAAEIVAGAVQDNGRALIVGTQTFGKASVQTVIPLED
                     GSALKLTTARYYTPNGRSIQAEGIQPDIVVKLVRPAEEPENPPGERLREKDLKGHIKP
                     AKEDTAKPDDNQNGDTRETTDLTRDNQLKTAIDILKSWDIMKKNLAK"
     gene            complement(42705..44177)
                     /gene="cysS"
                     /locus_tag="PLQ77_01545"
     CDS             complement(42705..44177)
                     /gene="cysS"
                     /locus_tag="PLQ77_01545"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPL09397.1"
                     /translation="MAQKIYNTKTRKKEIFQPVREGAVGMYVCGITAYDVCHIGHARA
                     AVVFDVVFRHLKARGYDVTYVKNFTDIDDKIIDRANREGVGIAEIAERYIALHDEDMA
                     ALNVLAPTVTPRATGHMEDMIALIRTLEEKGIAYAVDGDVFFAVAKFSAYGGLSGRNL
                     DEMMAGARVDVNDKKRNPLDFVLWKRSKEGEPSWDSPWGMGRPGWHIECSAMSRRYLG
                     EPFDIHGGGEDLIFPHHENEIAQSQAATGVPPAHYWMHNGFVKINAEKMSKSLGNIFP
                     IREIVRQFHPEVLRLFMLQSHYRSPVDYSDASLRESRTALIRCYTALRLLKDAQAAEV
                     HQGGAETASGGGEFAAKLRASVEKFDAALDDDFNTAQALGFVFDAARLVNQVMTVEKK
                     MPPADKQAILETAREAFRHFGDALGVFQSDPDLFFSKDRDIEAEKRGMDIHRVEALIA
                     ERQKARDAKDWGRADEIRRELALQSIALKDEAGRTTWIID"
     gene            complement(44191..45231)
                     /gene="argC"
                     /locus_tag="PLQ77_01550"
     CDS             complement(44191..45231)
                     /gene="argC"
                     /locus_tag="PLQ77_01550"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPL09398.1"
                     /translation="MKTKAAIFGASGYTGQELIRILSGHPQVELAAVTSRRYAGVKVS
                     DVFPSLAGLTFLTYTDASPREIAGICDVVFLALPHGVSMEIAPVFLEAGKKVIDLSAD
                     YRLRDPATYEEWYAKHSSAGLFGQAVYGLPELYRDAVKTSSLIANPGCYPTSIILGLA
                     PALKNKLLDVSTIIADSASGVSGAGRDPAVGSLFCEADGGFKAYKVGKHRHTPEIEQE
                     LNKLAGEKFFISFTPHLLPVKRGILSTMYASLRKEATQADLHALYASFYAGEKFIRIC
                     SAGTFPNLSSVCGSNYCDIGLTVDPRTRRVIIISAIDNLVKGAAGQAVQNMNLVCALP
                     EDSGLGAPPLYP"
     gene            complement(45277..>45419)
                     /gene="rpsI"
                     /locus_tag="PLQ77_01555"
     CDS             complement(45277..>45419)
                     /gene="rpsI"
                     /locus_tag="PLQ77_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPL09399.1"
                     /translation="KALLECDAELRPILKKAGFLTRDARIKERKKYGQPGARKRFQFS
                     KR"
BASE COUNT        10093 a        13330 c        12039 g         9957 t
ORIGIN      
        1 aggattttca gtccctcatc aattacctgc tcccggatgt ggacatccgc gccacgctca
       61 tcccctttta tgccgtatcc acggatttga ttaccggcaa acaggtcgtc ctgcacgaag
      121 gttcccttcg ccaggcggtg ctggccagca gttccgttcc cggagcggtt gaacccaccc
      181 gcctgggcga gtggcttctg gcggacggag gcgtgaccag cctggtcccc gtccacgcgg
      241 cgcgcgcgtc cggagcggat attgtcatcg ccgtggtggt ggaccgcgat ctgcctcagg
      301 aagcggacat cgaaacggca aaggacgttt tgtaccgcgc aggcgagatc accgccaacg
      361 cgctggaggc cgccgagctt caaaacgcgg acgtcatcat ccgccccgcc gtgggagacc
      421 tgcactgggc cgatttcagc cggtcgcata cgcttatccg gatcggggaa gaagccgccc
      481 gcgaatcgct ggaaaaaatt cacgcatctc ttccgctgcc ccggcgcttt gcccgctttg
      541 cccggcgctt cctgaaccgg gtgcagaatc acgacgcccg gcacaccggt tcaaatcctg
      601 atccgtcatg agattacccg gccaaatcca ttcctgcctc ctggcatcca tcgccttcgt
      661 tttgcttttc acggtgatgt gcggcacggt gtttgccgcc gggattgagg accggcaagc
      721 cgctgtccgg ccttggtcgg tttccctgca aacccggtat ttttttcaaa gccgcacctc
      781 ctatgaattt ggaaatccgt ttcctcccca tcaggcaccg ctcagccgtt tggaattccc
      841 gctggacagc tggtgggcgg gcgcgtcgat gcgccgcgaa ttttcccgtt tttcggcggg
      901 cgtggagttt ttccgcaacc tgtcggacga ggcgcggggc gtcatgatgg attccgactg
      961 ggacgatgcc gcacagccgg ctctcaaaac gatttattcc gaatcctcct gccgcatgga
     1021 gcccagctat agggtccgcg ccgacgcgga tttgaaaata tcggactgga tcggcctgcc
     1081 cggatggctg gatctgcggc cccttgccgg attggcctgg cagcgcttta cactggtcac
     1141 gcacgacggt tttcagtctg aaattggaaa ggggacggaa ttattgccgg gagacggcat
     1201 ccggttcgaa caaacgtact ggcagttctt taccggcttg aaaaccacgt tcgaactggg
     1261 aaagcctttt catctgacgc gcctcgcctt cacgatgcag ggagactggg cttacgtggc
     1321 cgggagcaac gaagaccatc acctgctccg gaccggcaac cgaatcaccc gcgagaagac
     1381 cacgggagac gcctggcatg ccctggtaaa tctgaaaatc gggttcacgc aaaacctaag
     1441 cgcgaatatc ggcgccgaat acctgcgcat caaaagcacc ggctcgcaca acctgaccaa
     1501 caacattttc gatattgatt tcacttttga tcacggtgtc aaggtttggt cggagcagtg
     1561 gagtgttgcg acggggctgg aataccggtt ttaagaaatg aggcgggtgt gaatgttgtc
     1621 accgctgtgt caacggatcg cgatccgccg caggaaacag cttcctgaaa aataaatcat
     1681 ttataccaca ggatacaaca ctcgcagcac catcagaaga cgacgcacat cttcatcagg
     1741 caatcctttg acaattcgac caattcgatt ctcgacaact gtcttatcaa tagtctcctg
     1801 ttcaaaacga aatagttcca gaggctcaac cttcatcgcg gcagcgattt taacgatagt
     1861 gtccaaggaa ggattctgtt gaccgcgctc aatttctcca atgaatttgt agcttaaatc
     1921 agcctgcctg cccaactctt cctgtgtcca tcccttgatg tttctaaggc ttcgaatcct
     1981 ccggccaaga agcaatctct cactttcgat ttgttttttc atatatgaat aatagatggt
     2041 ttaaaaacga cattaaaccc tgtattagtg ttattttatg ttgacaatgc actattaagt
     2101 gtgcttatgg aaaaaacaac atcacaggta tctaagaaga gttgcataaa aacattcaaa
     2161 ggcaaagaac atgaacggtg atttatcgct tcattcaaga ggttatcgca gacgctttaa
     2221 cgaaggtaag cgacatcacc aacattattt tccacagttt tattacaccc aataaatgaa
     2281 ggattgcgaa taatttgagc ttgatcacaa aacccataaa gcgatgcaac atagaatgta
     2341 atgtcgccgt gggcgaaaaa aataaaacaa ggaggcaagc atgaagaaaa gcattttttt
     2401 attatccatt ttagtcgcga tgatgtttgc agttgaagtt tatgcggaag cacccaagtt
     2461 tggcataggc gcaagagcag gttataattc ttatcgcaat ggaagcatgg aggtttcagt
     2521 cgcgggcttt ggagtggcag gaaatgttga ttattcaaat aacggggctt ggacgtttgg
     2581 atttaacgga attcttcaaa tcaacaaata catctccgct gaacttggac tagactttat
     2641 caattcatca aagaatgaat tcacagttgc cggcacgtct tggaaagctg gcgaaatcaa
     2701 acaaatgccc atcaccttga cggcacaatt ccattatcca gtaggaatat ttagcccgta
     2761 tgtgggacta ggtttgggat attatattag atcctatgat aaagataaca ctttctttaa
     2821 tcctgccgct gacgtctctg tcgacaatgg gttgggttat cacgccaatg cgggatcaga
     2881 catatttctc accaaggatc ggaatcttgc tcttaacgta gatttgaaat acgtttgggc
     2941 aaaaactgac attaccgcag ctgctggtgc ggcaaagcta gtgggaagca tgaaccttga
     3001 ttcgtttctt gttaattttg gcatcaaata ctttttttaa tcgttaagca acagtaacta
     3061 tggcccgcct agaacaggcg ggcttttttt aaaacaaatt ccaaaacaat aaatttcaat
     3121 ttaagtactg taacttagga tgacgatcag atatgcgata atttattatt tttctcatga
     3181 catgatcctc cattggacag cggctatgtt atcaattata tggcacatga ggtttgtcca
     3241 tgaaaacaaa aatcatattt gcatgcgcct ttcttctttt aatttcagga tgtgctgcca
     3301 aaacgcctct cataaaagct tcagcagaag gtgatacctt tgccacggag aaacttgtca
     3361 gagaaggcgc caatattgat gaagctgatg ataatggtta cacgcctttg atgcatgcaa
     3421 tctggtctgg gaaacaagaa accgttgcac agctagtcaa tatcggcgct gatgtaaaca
     3481 agcgtgacaa aaccggttat acgcctcttc tatgggcggc aagttatggt tatctggaca
     3541 tggccaaact gcttatcgac aagggagctg atgtcaatgc cagaggcaat gatcaaagca
     3601 gccctttatt gttggctttg gcggcaaata accatgaact atcaaagcta ctaatagata
     3661 gaggcgttga catacacgtt gtagatgtca atggaacaac gccgcttatc ctggccgcaa
     3721 tgtattgtga ttacgatatc gccaaaatat tcattgaaag ggatgtggat ttgtttgccg
     3781 tggatgcatc cggatacaag gcctccgact atgtaaaatt ttcattaaaa acggggaagc
     3841 gcgtttttca gtcggacgat tacgagcgta atttatcgtt tctcaaattg ataaagaaag
     3901 ccgaaaacgc tcaaatccta gctggacgaa tgaatacgaa gaaccccaaa atggctcgca
     3961 tatattcaat agtttataaa gtcggcaaat gtatcaaccc ggatgtagat tatgacgtat
     4021 ttatcagcga taaaaacgaa ccgaatgcct gggttaatat atctggcaat attacattca
     4081 caaaagaggc ccttgaaaaa tttgataatg atactctcac ttttgtggcg gcgcatgaaa
     4141 ttgctcatga taaactagga catgtcgcaa aaaaaatagc ggtaagttct acaattacgg
     4201 gcgcattggt cgtggcaaat gttttccttc ccggcgctgg ttatttgaat catatcatta
     4261 atccaactat tactaacaat tacagcaaaa cccaagaata cgatgcggat caaatggcat
     4321 cagatcattg cgctcaatgc tttgatatgt ccatcgaaaa acagatcacc gtaatgcaaa
     4381 aaatagaaaa aatatctaaa tcaagtggtg gtggtttttg ggcgacacat ccgtcatggg
     4441 aaaaccggat taaaaacgta gcaacgacaa cgaagtcttc ttcacagatt agaaacatca
     4501 aaacgacagc gcaatctcct tcgccgctaa tggaaggaga tcagaaacac aaagaaataa
     4561 aaggcatcgt gttaaatgat ggaaatgttg tcgaagggca actacttgaa atgagcacgg
     4621 acaccgtgat gattcagaca gaggacggaa aggtatcaac ctattcattt gaaaaagaag
     4681 ttcgtgggtt tgtcaaataa cagataaagt acgggggtac acgggcaggg ctcttcgtat
     4741 cttttcagaa aatcactaat gacattgatt aaactggctc acttataaac ttcctcaatg
     4801 aaaaacaagg aaaagcatca taacattgac atggtcgcgc tgcgtataaa atttcacctt
     4861 gcttggcata atcatataac ggtgatgttt ggttgtccca ctcttcaggc gtgaaaccga
     4921 caacctcaat cgggacaaaa acatcataga tggcttcgga cagtatatca atccttgacc
     4981 agtaatcatg gttccggaag tcttctgaaa tgaggatcag atcgatgcct gcctccgtcg
     5041 gcatgacgga aaaggctttc accctccctc aatccctccc ctcatactct aaaaagcgca
     5101 cgagggaggg aagatcaatc aggtagagcc ttttgatatt ctttgatcag cgcttttttg
     5161 ggaaggccgg cttcgatgat gtcccacggc aggaattcat ttacatcctt ttgacgatag
     5221 acgtagaagt cgggattgat ggtcacctct ttgaacgccc gcatccagtt gccgtttaga
     5281 cgatgcgcgg ccagcagaat gtcacccaca cgacgatccc cggaagacaa gagcgcctgc
     5341 acgtaggccc acttcggcac gtcggcgatg acgtgaatct gcttgtcgga gcgaaacgcg
     5401 tgggtgatct ttttgattct gcgccccaca tcctgaacct ccgccagcgg gcaccattgg
     5461 agcggcgttc gcggtttggg aataaactga ttaatgctca gcgtgatgcg ccggaatttc
     5521 cgttttcctt ttgtttgggc cagcgccgtg tgctggattt ttttgaccag atcgatgatg
     5581 gcgtcgatat cggaatcttc ttccgaaggc aggcccacca taaagtacag acgcaggttg
     5641 gacatttctt tttcaatcaa aagcttcgcg gcgttcagga tatcgtccgt tgtgatcccc
     5701 ttgcgcagca gatcgcgcag ccgctggctt cccgcctcgg gcgcgagggc gaccgtctcg
     5761 atgccgccgg tcttgagcag atctacaatg ctttcatcaa tcttgtccag gcgcaacgag
     5821 ccgatgcccg cctgagctcc gcgaccaaca atataccggc agatttttat caggtcggga
     5881 tgatcggaaa ccgccgtgcc gaccaggccg attttatttt ttatcatgag accgcgatcg
     5941 acggcggcca cgatttcttc atagccgcgg aagcgcggcg gcgcgtaaac aaaacctgcc
     6001 gcgcaaaacc ggcagaggtg aggacagccg cggttgactt ctacaagaaa catgtccgcc
     6061 atttccgcat cggcgccgct gacaacttcg gtcgtgcaaa aagcattaat atttctgatg
     6121 ggtgaaatct gaattttctt tggcagtccc tccgtgcgcg gcgcaatatc cttaatcttg
     6181 ccttcgggaa aataggacac cgcgtagagc gagggaacgt aaattcccgg tatttgtttt
     6241 tgtaaatcgt tgaacaagtc tttccggtcg agttcaagcc gccgggcttc agcaaacacc
     6301 cgcgcaaact gcggcaggat ttcctcggct tcacctaaaa caaagatatc gaaaaaatcc
     6361 gccagaggct ccggattgag cgtcagcgca atgccgccgc cgatcaccag cgggtgcccg
     6421 tccgcgcggt ccttcgattt cagcgggatg ccgctccatt ccagcatttt caggatggcg
     6481 gggtaatcgt tttcaaaaga gacggaaaac gccagaatat caaaatcgcg aagcggcttc
     6541 cggctttcca ggctgagagg ttccaccgcg ccggcggcgt attcggctcc gtcgccggcg
     6601 ggtagaaaca ctctctcaca aagaagggag gggagatcgt taattgcttt ataaaccgtc
     6661 tggaagccca ggttggccat gccgatccgg taaagattgg gataggccag gcaaaccgtg
     6721 aggcaggttc cccagacttt tttcatcacg ccccgctcgc gagccagcag ggcatcgtat
     6781 tttttctttt ccttccagtt catggacagt tcatggcctc ctttgcgatc cagcccgcat
     6841 tcggacagcc acgatgtttt cctagtccgc ctgccggcgc aggaacgtgg gaatgtcgat
     6901 atccacgttt tcgttttcgt cgttgacgcc catcgttacc acattggccg gatcgccgga
     6961 tttgctcttg cgcataaacg ccggcgttga cagatcctcc cgccggaagc cgcccagatg
     7021 cgtcacattg gagggaacct gcttcttttt ggcgtcctcg aagccggttg cgatcacggt
     7081 aatgcggatc tcatctttca tgttttcgtc aatgaccgtg ccgaagatga tattggcatc
     7141 ctcgtgcgcc tcggcctgaa tcagggacga ggcctcgttt acttcgtaca ggctcatgtc
     7201 gggaccgccg gtgatgctga gcaggatgcc ctgcgcgccc tggatggtgt tgtcctccaa
     7261 aagcggcgag gaaatcgccc gctgggccgc ctcgacggcc cggttctcgc cgctggccgt
     7321 gccggtgccc atcagcgcca tgcccatatt gctcatgatg ttcttgacgt cggcaaagtc
     7381 gaggttgatc agaccgggga caatgatcag atcggagatg ccttttacgg cctgatagag
     7441 aatatcatcg gccttcttga aggcgtccag cagtgaaagc ccccgcccgc ccaggctcaa
     7501 cagcctctgg ttgggaacca caatcagggt gtcgaccacg tcgcgcagtt gcacgatgcc
     7561 gtcttccgcc tgcgcatttc ttcttttgcc ttcaaactga aagggcttgg tgaccacggc
     7621 aacggtcagc gcgccgcatt cccgggccac ctccgcgatc accggcgccg cgcccgtgcc
     7681 ggtccctccg ccgaggcccg ccgtgatgaa gatcatgtcc gccccttcca ggtgccggcg
     7741 gatttcatct cgggattcca gcgccgcctg tttgccgatg tccggattgg agcccgcgcc
     7801 caggcccttg gtcacctccg ctccgatctg gattttcacg ggcgcgctgg acgctcccag
     7861 cgcctgggcg tccgtgttcg cgatgataaa atcgacgccc cgcagcgtat aggagatcat
     7921 ggtattgacg gcgttcccgc cgccgccgcc cgcgcccacc actttgattc ttgccgaatt
     7981 ctcgatgcat tcctccgtta attcaaacat gcacaccccc ttctttgttt agaaaaattc
     8041 cagaaaccat tttttgagtg ttttaaaata ttcgccgaat gcattccccg ttttccggta
     8101 aactgaatcg ccgggaatgt ttttactgcc gtaaacaatc aggccgacgc cgaccgcaaa
     8161 ggccggcgag ttcgctacat cgctcaatcc accgatgccc gctggacacc cgaggcgcac
     8221 cggcatgtca aagatctgct ccgccatttc gttgatgccg ggcagcagcg acgagccgcc
     8281 ggtcagcacg atgcccgccg ccagcaggtc ctcgtagccg gagcgcacga tttccttgag
     8341 cgccatgttc agaatttcat ccatccgggg ctccacgatc cggcccagga tctgccttga
     8401 aaccatccgg gcttcccgcc cgcccacgct gggcacttca ataacgtctt ccttcggaat
     8461 catcgacgtc atggcgcagc cgtgattcag cttgattttt tccgcctcgg caaacggcgt
     8521 gcgcaggccc gtggcgatgt ccgaggtcag aaagttgccg cccaccggaa gcgtagccgt
     8581 atgcttgatg cttccttccg caaagatggc catccccgtg gtcgcgccgc cgatgtcgat
     8641 cagtgcaacg cccagatcct tttcgtcggc gctgagaatg gcttcgctag cggcaagctg
     8701 ttccagaacg acatcttcga tatccaggcc gacgcggttg acggacttga ccacgttctg
     8761 aatcgaggcg gcggcgccgg tcacgatatg cactttggct tccagccgca cgcccgacat
     8821 tcccaccgga tcgcggatgc cgtcctgccc atcgaccaca tagttctggg gcagcgtgtg
     8881 cagaatttcc cggtcgacgg gaatggcgat cgccttggag gcctccagcg ccctctgcaa
     8941 atcgtcttcg ccgacttccc ggcctttcac ggcaacaatg cccagtgagt tctgtccttt
     9001 gacgtggctg ccggcgattc cgacataggc ggaattgatg cggcagccgg acatatgctc
     9061 ggcgtcttcc acggccttct tgatcgcctc gacggtgctg tcgatgttga ccacgccgcc
     9121 tttgcgaagc tccttggcgg gcgtaatccc catgccgatg atatcaatgc cggtcgtttt
     9181 tacctcgccg acaatcgcga tggttttggt cgttccaata tcgattccca ccagaacatt
     9241 gctttttttc gccataacgt cacccttctt cgtgaccgcc tatattctgt actgcggccc
     9301 ttttttctgc tcctgcgcct ttcccggcgc gtccttgcgc tgaacggtta ccttggaaat
     9361 atcggccaga tccacaaaca aacggccgtt cttcattccc cgcttttcca ggtccgccag
     9421 aacgacgtcc aactggcgca ccttgcctgt aaaattgtcc cgtcccagct tcagatgcaa
     9481 tccccggtcg gtcagcacgg acaggccgaa cacctcgtca atatgaattt cggaaatcgt
     9541 ccccagcgat gcgtacgaat cggcggacag aatctccagc agtttcagcg cctcggcaag
     9601 caggggagac ggcgcttttc caagattcac gccggtcagg atcgccaggt caacatcgtc
     9661 cgtccggctt aattttttaa agacgacgcc ttcagcatcc atgaggtaaa aattccccgt
     9721 ctgcttgacc agtgcaatcg gcgtccgttc gcgcacgtcc agcaccagcc gtcccggcag
     9781 ttcccggccg acataaattt tcctgaccca tggattggcg gtgatccgcc gcgccacggc
     9841 gtcggtgttg accgccagca gattgcagcg gggctgaatt ttcgccagct ccaggatatc
     9901 cttttcggtc aactccttca ctccgcgcac ggatatttcc ttgagctcca ggccgggggc
     9961 gcttaaaagc gcgttgtaaa gataaatcaa cacggcgctt gcggccacgg ccatcgccag
    10021 cagggccagg gcccccagcg attcgttcag cgccccgatc aaacgccgcc gcagacggtt
    10081 ttttttggct tccagattga cttttatctt ttggctcacc attcttctcc gatgacgaca
    10141 atttccgtct ccaggaaaag gcctttttcc ctttgaacca tttcctgcac cagccggatc
    10201 agcttcacca tgtcggacgc cgtgccctgc cccttgttga tcatgaagtt ggcgtgcttt
    10261 tttgaaatct gcacgtcccc cagccgccgg cccttcagcc ccagctcctc cagcaggcgg
    10321 cccgccggca ttcccggcag atttttaaaa accgaccccg cgctgggata ctgcagcgga
    10381 tgtttttcct ggcgccactt cataatttcc gccacccggt cccggatctg ctctgaacgg
    10441 cctttcgaca gggcaaatcg agccccgcag atgatgacat ttgcgggcag attggacttg
    10501 cggtaggaaa aagcgatctc ctcccgccgg agggtttttc tttctccccc gtcgtcgatc
    10561 aaatccgcga aaaccagaac gtccttgatt tcggcgccga atgctccggc gttcatccag
    10621 acggccccgc cgacgctgcc gggaatcccc gcgcaaaact ccagacccgt cagttcatga
    10681 tcggcggcca gcccgacaat cctgcccagc gcggcccctg cctgcgcttc gatccggaac
    10741 cgcccgtctt ccgcaggcgc gcacacggcc ctgtccaggc cgcgcagcga gatcagggcg
    10801 ccgcggtacc cgccgtcgcg cacgaggata ttggtgagat tcccgaccgg cagaaacgga
    10861 agatgattct ccttcagtct gcgcacaagt tccgcgagct gcacttcgct ttcgggcatc
    10921 accagcgcgt ccacccggcc gccgagtttc agcgaagcgt ggcgcgatat cggctcgtca
    10981 aaccatacct tgccgacatt cgcgccctga agcgccgcgg aaattttcaa atgaccggtc
    11041 atggattctt cgttccctct ttgagccgcg cgagaaacgc ctcgccgatt ttatacacgc
    11101 tgcccgcgcc cagcgtcagg acggtgtccc cgggctttgc attggccagc agaaattcga
    11161 tcgtgccctg cggctgggcg atgtattcca cgcagggatg ccccccctcc ttgatcgcca
    11221 cccacagcgc cgcggaatga acgccgggaa tcggcgtctc cgacgccgga taaatatcgt
    11281 tgagcaccag cacgtccgcg tccccgaacg ccctggtgaa ttcgtcgaac agcgctttgg
    11341 tccgcgtaaa gcggtggggc tgaaacacga cgatcagccg gcctcgccgc atctggcgcg
    11401 ccgccgccag cgtggccata atttccgtcg ggtgatggcc gtagtcatcc accaccgtga
    11461 tcccggcctt ctcccccttg acttcaagcc gccgctgcac gccggaaaaa gccagcagtc
    11521 ctttgcaaag ggtcgccatc tccagaccca gttctcgccc cacggcaatg gcggccatgg
    11581 agttgtaaac attaaagagg cccggcacca tcaaggcaac gtcgcccagg ggttcgcccc
    11641 tgtaagacag ggcatagcgg gttccggcgt cggaatgccg gatgtcgctc gccgtataat
    11701 ccgccggctg tgaaatgccg tacgaaatca cgcggcgttt gatcgagcgg gcaatggcgc
    11761 gcacatgctc gttgtcgacg cacatcaccg tgcagccgta aaagggaacc atgttggcaa
    11821 atttcaggaa agcgtccttg atttcttcaa tgcccggata atagtccaga tgctcgcggt
    11881 cgatattggt gatgacggcg atggtcggcg acagcttcag gaaagagccg tcgctctcgt
    11941 ccgcctccgc gacgaggatg tctccgccgc ccaggcgggc gttggatccg atgctggcca
    12001 gcttgccgcc gatcaccatc gtggggtcca gtccgccctc cgccagaagg gttgaaatca
    12061 tcgaggtggt cgtggtcttg ccgtggctgc cggagacggc cacggaaaat ttcattttca
    12121 ggagctccgc cagcatttcc gcccggggaa tgaccggaat attcttgcgg tgcgcttcca
    12181 gaacttccgg gttgtccgcc tttacggcgg tggaggtgac caccacgtcc gcgccgccga
    12241 tgttttccgc cgtgtgcccg acggcaacgg ccgcacccag ctgctgcagg cgcttcgtcg
    12301 tgtcggagga ctgaacgtcc gatccgctga tgccgtatcc gagattcagc agcacctcgg
    12361 caatgccgct cattccgatg ccgccgatgc cgacaaagtg gatgtgcttg actttccgct
    12421 gcatgaatcc ggtttcctgg tctttgctca tgttcctctt tcctgtgtgt ttttttgttt
    12481 cgcccccagc cgcaggcagg catcgacgat ttccgcggcg gcgccgggcc tgcccagcgc
    12541 tttggatttc tcttccatgc cccgcagctt ttcgccgtct tccagcagag tctccacgag
    12601 caaatacagg gtcccggcgg tcaacccgct ctccgggatc atcgccgcgg cacctgcccg
    12661 gaccatcgcc cgcgcattct cggtctgatg gtcgttggcc gcatacggga agggaatcag
    12721 cacagacgct tttcccgccg ccgtgatttc cgccagcgat gtcgccccgg cccggcagat
    12781 aatcagatcg gcagacgcgt aagccgacgc catatcaacg atgaacggtt gaacatcagc
    12841 ctccacgccg gccttcccgt aggcctgacg gaccgccgcc aggtcgcgct cgccggtctg
    12901 gtggaccaca cggacccggc ggcgcattcc ctgcagcatc ggcagcatgg ccacaaccgt
    12961 tttgttgatg gccgctgctc cctgcgaccc gccgaaaacc agaatctgac ggaaggcgcg
    13021 cgaagtcggg gcaacaacga gacccgcgac aaacgccgcc cggaccggat tgccggtgac
    13081 gcggactttc cggacatcaa atcttccccg gctttgctca taggtcaaaa atatttgatc
    13141 gacaaatttc cccaaaatgc ggttcgtgat ccccgccagc gcgttctgct ccgcaatggc
    13201 ggtcggaagc cccatcagcc tggccgccag gacggcgggg cctgaggcat acccgccgac
    13261 accgaggact gcatcgggcc gaaactccgc gagaatccgc cccgactgga tcatgctgtt
    13321 cggtatcgcg taaacgccct tgatcaaagc cttcaaaccc cttcccttca acccttccac
    13381 atcaatgagc ttcagctcgt atcccagcgt tcccagaagc cggtgctcga tgcccctgcg
    13441 ggttccgata aagatcaccc ggttttgagg atcgcgcttc agaaattctt ccgcgacggc
    13501 tatgcccgga aacagatgac cgcccgttcc tcctcccgca atggctatcc tcaccggttt
    13561 tcccccgccc gcgcgtgttg tcttttttgc gccgtttacg tcctctgcgt ggagatgttg
    13621 agcaaaatcc ccaccgtcat catgctcatc acaaacgacg agccgccgta gcttaagaat
    13681 ggaagggcca gccccttcag gggaatgagc cccatcaccc ccgcgatgtt gatgaaggcc
    13741 tgcagcgcga tcagcatcgt cagtccggcg gccagcagcg ttccgaagag attcggcgcg
    13801 cgaaaagcga tcataaagcc ccgaagcatg aagaaaatga acaggacaat tataagagcc
    13861 acaccgataa aaccgctttc ctccgcgatc accgccagaa taaaatccgt atgcggctcc
    13921 ggcagataaa acagtttctg catgccgtcg ccgatgccct gccccgtcac gccgcccgaa
    13981 ccgaaggaga tcagcgactg gatgagctgg aaaccggttt tgtccgcgtc tttccacgga
    14041 tcgagaaacg ccgtcagccg cgccatgcgg tatcccttgc tcatgatcag ccagatgccg
    14101 acggggataa aggccgcgcc cagaaacatc aggtgagtga tgcgggagcc ggccagggac
    14161 agcatcaaaa gcagaatcga catcataatg acgaccgtgc cgaaatccgg ctgaagaata
    14221 atcagtccca tgaccacggc cgtcaccgac aggggaatca ggacaccccg gctgaatttt
    14281 ttcaaatggt gcactttgcg cgtcagcagg tgggccagga aaagcaccat ggccaccttg
    14341 accagttcgg tcacctgaaa ggaaaacacc cccagattga gccagcgggt cgcgcctccc
    14401 cgcttcacgc cgacgtgcgg aataaaaagc aacagcagca ggaccacgga gacgatcacg
    14461 ccggggtacg ccagcttctt gagctgttcg tatggaattt tggtcataac gatcatgatc
    14521 accaggccgg cgaatacaaa aaataaatgc tttttgagaa agaattgtcc gtctttgaat
    14581 ttttccatcg ccagaatgga gctcgacgaa taaatcatcg ccgtgcccac ggtcaccagt
    14641 atcagggtga ccagcaaaag aataacatcc ggcgtattgg cgtctttttt cgcagcgtcc
    14701 atttaaagat tcctgaccac ttccttaaaa tgatttcccc gctccctgta attggcaaac
    14761 gcatcaaagc tcgcgcatcc cggcgacaga agcacaatat ctccgggttt cgcgtcccgg
    14821 tacgcccgga ggacggccgc tttcagcgtt gcttccttcg cgatcggcgt cgtattgccc
    14881 agctgcggct cgatttggtc gcgcgcctcg ccaaacagga caacctgttt ggttttggcg
    14941 cccagcatcg gctgcaacac gtcaaaattt ccgtccttgt cccgtccgcc cagaagcaga
    15001 atcaccggcg acgaaaaggt ttgcagcgcc cgcacgacgg catcaacatt ggttcccttg
    15061 gaatcgtcat aaaattgaac cgcacgcttt tccccggcaa attcaatccg atgcggcagg
    15121 ccccgaaact gcgacaccgc ttccacaatg ctctcctttg aacagccgca gaagcgagcc
    15181 acggccaccg ccgccatgac gttttccaca ttatgcagac cgggaatcct gatcatgccc
    15241 agagggtacc gctcatccgt gccgtcgggc ccgctgtaca ccagagaatc cttctgcagg
    15301 caaaggcccg gcgcaagcgg ccggacggag ctgaaccgga ccacgcaggc ccgcattgaa
    15361 gcatccaggc cttcctgctg cggatcttcg gcgttcagaa ccgccagatc gttttcacgc
    15421 tggttttcaa aaattctccc cttgatgcgg cggtattctt caaacgaccc gtggtagttg
    15481 acatgatcgc aggtgatgtt gagcagaatg gcggcatgcg gacggaacga ttcgatccac
    15541 tgaagctgaa agctgctgat ttcggcgacg atgaaatcat ccgtctgcgc ggcgccggcg
    15601 tattcgatca gtggattccc gatgttgccg ccgacaaaca cttttttccc ggcgcgcgcc
    15661 agaatctcgc ccagcagcgt ggtggtggtg gttttcccgt tggtgcccgt cacggcgatg
    15721 atcggcacgc ggatgaaccg gcaggccagc tcgacctcgc tcaagatcgg aatctttttt
    15781 tgctgcgccg ccgtcagaag gggatggcgg ggggggatcc ccggagacgg caccaccaga
    15841 tccacgccgt ccaggcaggt ctcgtcatag cgtccgacgg caagccacgg ccgatgggcg
    15901 atttgttcaa actccccgcc ccactggtcc cggggtttct catcgaccgc cacgacggcg
    15961 gctccgcttc cacccagaaa cgcggcggcg gcaatgcccg ttttccccat gccgatcacg
    16021 gctattttcc tgtctgccag ttgcatgctt tccctaccgc agcttcaatg tgctgatcgc
    16081 cagaagcgcc agcaggatcg aaatgatcca gaaccggaca ataaccttcg gttccgccca
    16141 gcccttgagc tcaaaatgat gatggatcgg cgccattcga aaaatccgtt tgccgcccgt
    16201 cagcttgaaa taccctacct ggaaaataac ggaaaacgtc tccattacaa aaactccgcc
    16261 gacaatcgcc agcagaattt cctgtttggt gatgagcgcc acggccccca gagcgccccc
    16321 catggacagg gatccgacat cccccataaa aatttccgcc gggtgggcgt taaaccacaa
    16381 aaagcccagc gccgcgccca tgaacgctcc gcagaatacc gacaactcgc ccgcgccggg
    16441 cacataggga atctgcagat aatgcgcaac cttgatgttg ccggcgaaat aggcaaacag
    16501 cagatacgtc gcgaagcaga tgcccgcggg cccggtggcc aaaccgtcga ggccgtccgt
    16561 cagatttacg gcgttggccg cacccacgat gatgaaaacg cacagcagga tgtagcccca
    16621 gcccagattg ggcaacaccg tcttgaagaa aggtatcgtc acctgcgcgt tgaaccccgg
    16681 cttgaaatac aaaacaacgc cgacgaacag ggcaatgatc atttccgcgg tcagcctcgt
    16741 tttcccggac aggccccggg agtttttgcc ggccagtttc cggtaatcat ccagaaagcc
    16801 gatgaggccg aacccgacgg tgaccagcag caccagccag acatacacca cggaaaggtt
    16861 cgtccacagg atcgtggaaa ccgtaaccgc gaagatgatg agcatgcctc ccatcgtcgg
    16921 cgtacccttc ttgaccaggt gcgtcttggg cccgtcctcg cggatctgct ggtcaatctg
    16981 cattctctgc agcttgtcga taaaccattt cccgaaaacc aggcagatca gcaacgccgt
    17041 gatggaggcg aagatcgttc tgaacgtgat gtaccggaac acgttgaagg atgaaaacac
    17101 ggtatggaaa ggatataaaa attcgtaaat cacgtttttt ctcccggact atgtcgccgc
    17161 cgcatggtct tcggcttttt caacgccgat ttcttcgccc agccgcgccg ccagcctgtc
    17221 cattttcatc cgccgcgatc ctttgatcaa aacccagtcg cctttgcgca gcttcttttt
    17281 caagaaagcc acggcttccc cgtcgtcgca taaaaacaca atccgttctt cggccagacc
    17341 gccctccagc gctcccgcgg ccgtcgcgcg cgcgaaatcg ccctgaagaa aaaccgccgc
    17401 cgtgccgatg gtcgccaaaa gcaggccgat cttgcggtgc atctcctgcg cggcgtcgcc
    17461 caattccagc atgtcgccta aaaaaacgaa cgcgttgtgc gaacttgcca ggtccctgag
    17521 cgtcagcagg gcctcgcgca cggatgcggg attggcatta taggcgtcat tgatcagata
    17581 ggctccgttt tgcagccgga gaatctccat gcgtccggcg accggctgaa acatcgacag
    17641 gccccgccgg atcgactcta cgctgacgcc gcaggccaat gccgcggcgg acgcggccat
    17701 ggcgttgtag atgttgtgaa tgcccgcggc tttcatgtcc accttgtgcg cctgcccgtt
    17761 catcaaaagc tgaaaactca ctccccgggc gccgtttttg cggatgtcgt tcacgccgac
    17821 atccgcgccg gcgctcatgc tgaaggtcac gcgccgtccg gaccagcgct cggctgcctg
    17881 gcgcaccgcc tggtcgtcca gattcacgac agcgacgccg gccggattca tgtgcagcca
    17941 taaatcgcct ttttcctccc gcacggtttc caccgagccg aaccccgcca gatgcgcggg
    18001 gccgacgttg gtgacaagac cgatgtccgg ggcggcgatc tgcgtcagcc ttttgatttc
    18061 gccgcgcgtg ttggtgccca tttcaaggac ggccgcatcg tgccggtccg tcattcggaa
    18121 aagcgtctgc ggaaggccga tcaggttatt caggtttccc tccgtgccga gaacggtttt
    18181 ctcctgccgc aaaatgcagg tgagcatttc acgggtggtg gtctttcccg acgacccggt
    18241 cagcgcaatg acgggaatgg aaaaacgacg acggtgcatg tgggccaggt ctcccagcgc
    18301 ccgcagcgtg tctgccgttt caatcaccgc ggcccgccgg gtcagcgcct tcaaatcgat
    18361 tctgtccgcg tcggccacca gaacacatag ggctcctccc tccacggctt ttggaacgaa
    18421 ggcgtgtccg tcaaaattct ccccggcaag ggcaacaaac agattgccgg gatggatttg
    18481 tctggaatcg gtggaaacgc cgtaaacggc ttgatcgcgc tctcccgccg ccaggcgtcc
    18541 gccggtggct tgcagcacct ctgccagcga aaacgccggc gacatccacc cgggaaactt
    18601 cagccgcaaa gcgcgcatgg cggccagccg gtcgtcaaac ggcacttttt tcgttcccag
    18661 gatctgataa tcttcatggc ctttgccggc aatcaaaacg atatcctgcg gttcggcgat
    18721 ccggacggcg gcttcgatcg cggctctccg gtcggcaagg atcgcgtatc catgcgcgcc
    18781 ggcgtcaaat tcaaggcggt cctgaggcac tttcctgatt ttgcccccat cgattcccgc
    18841 ttcaatttca cggatgatct ccagcggatc ttcccgccgg ggattgtcgg acgtgatgat
    18901 cgtcaggtcg ctccaggtcg tcgcgatgtc tcccatcacc ggccttttgg cccggtcgcg
    18961 gttgccgccg cagccgaaaa ccgtcaggat tcttttttcc ttcaacccgt gcagattccg
    19021 gagcacctgc tccagggcgt caggcttgtg ggcgtagtcg acaaaaacgt gaacgccgcc
    19081 cgaagggacg accttttcca ggcggccggg aaccagcgac aggttctgaa tgccccgtgc
    19141 aatcgcccgg ggctccaccg ccaggaccga cgcggcggcg gcggcggcca gaatgttgga
    19201 caggttgaat ccgcctatca gaccggacgt cacggaaatt ttttctccgt ccagcaggat
    19261 gtccgcctga acgccgccca gcgtgacgtg tggattctgt actctcgccg cgccgtcctg
    19321 ctcaaggctg taggaaagag ccggaacgga aatttcgcgg atcagctgcc gcccccgggg
    19381 atcatccgcg ttgatcacgg cttttcgcgc aaggttcttc cggcttcggg gaagcaattc
    19441 ggcaaacaat ctttttttcg cccggaaata gtcttccatg tctttgtggt aatccaggtg
    19501 ctccgggctg aggttggtaa aaacggccag atcaaagtcg cagtcgtcaa cccgcttcag
    19561 atccagtgcg tgcgacgaaa cctcggcaat cacgtgcgtg accccggcgc aggccatctc
    19621 gcttaaaatt ctctgcatgt cgaaggattc cggcgtcgtg ttcggggcgg ggaattgttt
    19681 gccgccgtag cgataattga tggttcccag aacgccgcag gaaaagccgg cggccgtgag
    19741 aatggattcc agcaggtagg acacggtggt cttcccgctg gttcccgtga tgccgatcag
    19801 cgtcagacgg gaggacgggt ccccgaaata atttttggcg agcgtaccca gtgcgcggcg
    19861 actgtcgggc acccgaatgg ccttgacccc gtcgggaacc acgatgtctt tctgatggac
    19921 gatgaaatgc gcgccccgct ctatggcccg gccgataaaa tggtggccgt caaaggcaag
    19981 gcccggaatc gcaacaaaga gcgaccccgg gccgcacgcc tctgcgacat agcaaatcga
    20041 tgaaacatcg cctttcggat ccgtttccgt ctgaagtgtc tggattccct gcagtaattg
    20101 acgaagctcc atcatccctc agtttttcat ctcaaaaatc actttgcaca ggcgatcctc
    20161 gcccggttcc gaaccgggct ggggaaactg ctgcgtcgcc cagccgcttc cggatacccg
    20221 cagctcaatc gaccgggacc tggccatttt cagcgcctcg cgaatggtca ggcctttgaa
    20281 atccggcatg cgggattcat cattgatctc attctccgct gccgtcatgt cttccaccag
    20341 ggacgcatcc gccgacacca gctgcagttc atggaccggc tggggttcaa acgcgggggt
    20401 ttcccgaatg ttcgtcttga aacagttcag aatctgctcg ccaatgcttt taaaaaccgg
    20461 cgcggcggcg acgcctcccc acttgtcgcg ctgcggctca tccagcatga ccagaatcac
    20521 cacctgcgga ttatccgacg ggaaaaatcc gataaaggac gtccgcacct tttccgagga
    20581 gtaaacacct cgcgcaaaat caaatttctg cgcggttccg gtttttccgg ccacgggaac
    20641 attgacgata tgggctctct tccccgtgcc gtcctcggcc ccgaccacct cggtcagcat
    20701 gacggccatg cgcctggccg tttcgggaga aatcacccgg cgcacggtct ccggagcgaa
    20761 catcttgacg ggctgattgt ttctatcggt gaaccccttc acgatatagg gtttcattcg
    20821 aacgcccccg ttggcgatcg tggccagagc ggtgacgagc tgaatggcgg tgaccgaaat
    20881 gccctgaccg aaaccgatgt ttgtcgtgtc cacccgggtc cagcttttga cgggacgcaa
    20941 aacacccgcc gcctcgcccg agagatcgat tccggtccgg gagccgaaac cgaagttttt
    21001 tatgtaggca taaaactttt cccggccgag cttttccgca attttggcgg agccgatatt
    21061 gctggagtat ttgataatat cccgcacggc cagataaccg tgcttcttgt ggttggcttc
    21121 gcgaatcacc cggttggcga cctgataatg cccgttctcg cagtaaaagc ggtccgactc
    21181 tttgacgact ttttcttcca gggcggcggc gaccagaaag ggcttgaagg tggagccggg
    21241 atcaaaggaa tcggtaatgg cgcggttccg ccagttctcc gacgtcagcc ccttgatgtt
    21301 gttgggattg aatgcttttt cattggccat cgccagtatt tctcccgttt tcggatccat
    21361 gacgatcacg acgccgcctt tcgctccctt ctccagcact gcttccttca aatgcgtttc
    21421 cgccaggtac tggatccggc tgtcaatggt cagcacaaga ttggcgcttc ccattttctc
    21481 ggagggatcc tgttcgacgt gcacaaagag ctttttccct ttggcgtccc tggcccaggt
    21541 cagtttttcg ggcgttcctt tcaaataccg gtcatatttc ctctccagcc cttccaggcc
    21601 ctcggcgtca aaaccggaaa atccgatcaa atgcgccgcc aacggcccat tcggataaaa
    21661 acgcttcggt tccttgatca gaaaaattcc ttccaggttc gcggattcca ccatcagcgc
    21721 ctgctgggaa gaaatttttc tggccagcca gcagaagctc ttcggctccg aaatcttctt
    21781 cagcacggat gagggatcca ggttcaggat ggaagaaatt tcccgggaca cttttgccgg
    21841 attgctgatc cggctcggat ccgcgcaaac ggactccgcc atcaccgaca tggccagctt
    21901 ttcgccgttg cggtcataaa tcatgcctct ttcggcatga atggaaagcg tggtcatgtg
    21961 ctggcggtcg gccagtcttt tcaatttctc gccggacagg acctgaagct gaaaagcgcg
    22021 cgacagcaac gcgatgaaca gaatcaaaaa aatcgccaaa atgctggcca gccggaattt
    22081 cagccatttt tttgagtctg ccgccatggt cacttccttg tttgctttaa aacaatcacc
    22141 tgcgccgccg tgggataggt catctgcagc tgcgttcggg caatgtcttc gattctctgc
    22201 ggcgatttga gcatcgccag ctccagcttc aattttttct gctcttccat caattgctct
    22261 ttggtatgca gggcggcggc aacgtcatat tccatttggg tcatccggat gtgcgatccc
    22321 acatagatca ccgcgacaaa catgatgacc agcgccacga cgatgaaggc cggatatttt
    22381 acgccgaagg aagcggcgtc ggcttctttg agaggccgcc cctgcgtcag ggtctcggtt
    22441 ccggccgtct gcgccatttc aaatcctttc tgcgacgcgc aattttgcgc tgcgggcccg
    22501 cgggtttctc cggatttcct ccaaagaagg cgtcaccgct ttgcgggtta atactttcac
    22561 gacggcctgt ttgtgacaga cacagcgggg aacatctttg gggcaaacac aggtcgccgc
    22621 aaggtcacga aaagtatgct tcacgatccg gtcctccagg gagtgaaacg aaatcacccc
    22681 cagacggccg ccggtcgaaa gagccgcgac ggcagcttca atgcccggag ccagggtgtc
    22741 cagctcgcgg ttgacggcga tccgaatcgc ctgaaaggtt cttgtggccg ggtgaatttt
    22801 ctgatgcttc atgctatgcg gcattgcctg cgtgatgagc tgcgccagct ccgccgtggt
    22861 ttcaatcggc gaaacatccc ttcttctcac gatcgctccg gcgattcttg cggccatttt
    22921 ttcttccccg tacaaccgga tcactctttc cagctcggac ggtttgaaac cgtttacaat
    22981 atcataggcg cgaagctttg catcccggtc cattcgcata tccagcggcg ccgactggct
    23041 gaaactgaac cctctgtgcg ccgcatccag ctgatgagaa gaaaccccca gatccaaaat
    23101 cacgccgtcc actttttcaa tgttgaaccc gtccagcaca gaggccagat cggcaaaatt
    23161 ccccttgacg agtttgacgc gcggctgaaa tgaatccagc ctttgccggg cggccgagat
    23221 cgcttccgca tcgagatcga tcccgaccag actcgcccgg gtgttttcca ggatcgcgag
    23281 ggcgtgtcct cccccgccga tcgtgccgtc cacataaacg ccgctctgat ttctcaccag
    23341 ccaggacagc acttcttcgc gcatcaccgg ttcgtgagaa aaaaccaagc tcatggtcag
    23401 atccccagtt gctctgcgat ctcattatct ctgtcaattt tctcgccagc ctctcgattt
    23461 ctttcttcaa atttatctct gggccagatt tcaatatttt taagcatccc cgccagcaca
    23521 atgtcttttt ccaaatgagc gtattcccgc agcgtcggcg gcaccaaaac ccgcccctga
    23581 ctgtccagat tgcagtccac ggcgccggac atgaagaatc tttgaaaatc gcgaaatttt
    23641 ttatccagaa ggcgtctttt cgagacttct tcttcgataa gccgccattc cgcataggga
    23701 aatgcaatta aatacccgtc ccagttggtg attaccatgc ggttgtcgta cttttccgca
    23761 aacagttcgc gaaacctgga cgggaaaatt attcttccct tttcatcgat agtatggtga
    23821 tattgcccac gaaatacgga catcacgccc cctccacaat aaaccacttt accccacgtg
    23881 tccgactata gagagggaaa atcgctttgt caagaaaaaa atgaatcttt tttgataatc
    23941 gaataattga atatcactaa gaaaatggga aacgcgcccc ggtggagcca atcggtctgc
    24001 aaaacagcac ggacaaggcc gccgggaaga gaaaaggatc ctcccggcac actccaaatc
    24061 acaaaccgga ttataataat aaaaaagaat tatgttaaat agttagaagc agaaaggcgc
    24121 tgattaccgg acgacttcat ccgttttctc ttcgcggttc atcatattgc gaaaacggat
    24181 tgttgcgtca ttgtgggcca caagcgatga cgaaaaaaag tgggtcccgt cccctttgga
    24241 aacaaaatag agataatcca cacccgcggg attgagaacc gcctgaaaag aatccagccc
    24301 gggactggca atgggccccg gtggcagacc gcggatctgg taagtattat aaggagattc
    24361 ccgcttatag tggaatctct tcacttttcc cttgaaattc ttgaggtcgt aaaccgccgt
    24421 gggatcacac tgaagcggca ttcccttttt caaccgattg tagaaaaccg ccgcaatcat
    24481 tggtttttca gcggcatacc ccgattcctt gcccaccagc gacgcaaaag tcacgaactg
    24541 atttgcattc aagccttttt gcgcggcctt gcggatcatt tccggcgaga ccctgctgtg
    24601 gtaacgctcc accataatgc gcatgatctg ccgggtggtc atggaccggt ccagaaaata
    24661 tgtgtcggga aagagatgcc cttcaatcga agcactctga atgtccagga aagataaaaa
    24721 ttccctgtct tgcgccagct caaaaaaatc cttttcgttg atcaatttgt attccctgag
    24781 acgcgaggcg acctgcgaaa tcgaactgtc ttcaaaaatg gtgacgcggt attgcttgat
    24841 ttctccgtga agaagcttgt ccacgagcac ggaaggggaa atgtctgcgg caaattcata
    24901 ctctccggcg cgaaccgacc gggcccctcc tttcaccacg accagcgcat gaaataaaac
    24961 ccggttttgc accaggcctg cattgtggag aatattgatg acccgaatca ggcttgtgcc
    25021 tgtcggaata tccaccacac cgcttttgtc tttcgccgaa tccatcggcg tcacggcata
    25081 gatgaagagc agcgcgccaa acacgacggc cgcgatcgcg ggcagggaaa aaaaataaaa
    25141 cagtgttttt tttgttctgg atttcatggt cattccattt gccgaacaag gcgagtccgg
    25201 cggggatcag cccggctcgc gttttccggc attgtccaga taatcctgca gaatgagaca
    25261 cgccgccagc cgatcgactt tatccttcct tttccgccaa tggacgccgg aggccctcag
    25321 aatgtcttcc gcctcttttg tggtcagggt ctcgggccat ttcaccaccg gcagcatgaa
    25381 ggtttcggca agccgcccgg caaaccggtt gactttttca cactgaatgc cttccgatcc
    25441 gtcgatgcgc accggatagc cgatgactat gcgctcaacc gagtaatcga ggatccgccg
    25501 gcgcaaaatc tccagatcat tctttttggt tttgcgaatc agcgtgggca gtccctgggc
    25561 ggtcaggccc agcccgtcgc tcaccgccag accgattcgc ttttcaccgt aatccactcc
    25621 caaaatgcgc aaagatcacc tcgtggatgt tttctaacaa ttccaccctg cattttcaag
    25681 aagtaaatca aggcccgttt ccgtagctca gccgctccgg caaatatcag gttgttaaaa
    25741 tgacgggagt ttgttatttg aacgtatctt gaagcgcgaa ccagcgggaa aaaacatgca
    25801 gcatttcgtt ttgggaacag ccggccacgt agaccacgga aagacatcgc tcatcaaggc
    25861 cctgacgggc gttgacaccg accgcctgaa ggaagaaaag gaacggggca tcaccatcga
    25921 actgggattt gcctctcttt cgcttccctc cggccacacg ctgggcatcg tcgacgtgcc
    25981 gggccatgaa aagttcatcc ggaacatggt ggccggcgcg gcgggcatcg acctggtttt
    26041 gatggtcatc gccgcggatg aaggcgtcat gccgcaaacg aaggagcacc tgcaaatctg
    26101 ctctcttctt ggcattacga cggggatggt cgcgctcacc aaaatcgacc tggtggaaca
    26161 agactggctg gaactggtac gatcggaaat cagcgaatat cttcagggca cgtttctcgc
    26221 cggcgccccc attgtgccgg tttcagcgtc tcaggggcag ggcatcccgg cgctgcttga
    26281 agaaatcgac aaaaccgtcc gccggctcgg ggaaaaagcc gacgacggca tattccgtct
    26341 gccggttgac cggatcttta cgatgaaggg cttcggcacg gtcgtcaccg gcaccctgat
    26401 ttccgataaa atcaaaatcg gtgaagaagt gcagattctg ccggaaaaga tatccgcgcg
    26461 cgtccgcggc atacaggtgc acaaccaatc cacggaagaa gccttctccg gccagcgcac
    26521 cgcgctgaat ctccagggca ttgaaagatc gacgatcgaa cgcggcaatg ttctggtgcg
    26581 gcccgagacc gtctggccca cccagcggct ggatgtgttt tttgaatacc tggcctccaa
    26641 tgcccgaaag ctgaaaaacc gttccctggc tcgtctgcac accggcacgg cggaaatcat
    26701 ggtgcggttc gttctgctgg atgccgacga actttccccc ggcgacgccg ccttcgccca
    26761 actgattctg gagaacgagg acgtggtggt agggggagac catttcgtgc tgcgcagcta
    26821 ctcccccgtc accaccatcg gcggcggacg gatcctcgac ccgcatcccg gcaagcacaa
    26881 acggaaaaat cagaaaattc tggacgatct gaggctgctg caaagcggcg cattgccgga
    26941 gaaaattctc gtgatgctgg atcgcgcggg ctttcacggc atccccatcc gctctctcgc
    27001 gtttcgcctg ggcgcgtccg tcaaaaaaat ccgggaagcg ctggaaaaat tattttccga
    27061 ccgccgtgcc gtcctgctct ccaacgaaga cgccacggcg ctttccgcgg gcctgtacgc
    27121 gcacctggaa gagctcctga tcaaaagtct cgcgaattat caccggagcc atcccttgaa
    27181 ggaaggcatt tccaaggagg agctcaaggc ggcgcttccg gaggcggtat ccggaaagct
    27241 tttcaacatg gtgcttgccg gacttgccag gaaggaacgg atcgccagcg acaaggacaa
    27301 tgtgcggctg gcgtcccatc acgtgcagct ggagggggag gaagacgctc tgcgccaggc
    27361 catcgcatcc gtttacgcgc aggcgggact ggcgccgcct tccctttccg acgtgatggt
    27421 tcgattcagg gatcagaagg ccagggcgca aaacatcgtc cggctgatga tcaaagacgg
    27481 cgatctggtg aaaatcaatg aagagctctg cttcgcgcgc gcggccatgg acaaattgcg
    27541 tgaagactac aaggggcagc tgatccggga cggtcaggcc acgccggcaa cgttcaagga
    27601 tttaaccgga ctgtcccgca aatacattat cccgctcatg gagtattttg acgccagcaa
    27661 actgacggtg cgagtggaag accaccgaat cctgcgggaa aaaccctgag acccattttt
    27721 cagaaaggcg gtgcatttga aaaccaaaga cctctatctt caggacatcc ggcccggcga
    27781 taaaattgct tcttcgtttc tggtcacgga aaaaaatatg gccttttccc agaaagggtc
    27841 ggcctacctg aatgtccggc tcaaggataa aacgggcgaa atcgacgcca aggtgtggga
    27901 caacgccgtg gcgctcgacc gcgttttcaa aaaaggagac atcatttcca tcgaaggccg
    27961 ggcgcaaagt taccgcaatg ccgtccagat ttccatcctc acggccaaaa cctgcggccg
    28021 ggaggacgtc gatccatcgg actacctgcc ggccagcaag ctggataccg cggcaatgtt
    28081 cgaagacctg cgcgcttacg tcggcacgat gtcctccgag ccgctgaaaa aactgctgga
    28141 cgcctttttg aacgacgaaa aaacggccga cctgttccgc cgggcgcccg ccgccaaagg
    28201 atttcatcac atttacctgg gcggcctgct ggagcacacg ctttccgtgg tccgcctgct
    28261 cgaccgcgcc gccgaacatt atcctcaatt aaaccgggat ttgctgatcg cgggcggcat
    28321 cctgcacgac atcggcaaaa tttatgaatt ctcctatgat ggaatcattg aatacagcga
    28381 cgaaggccgg atgatcggtc acctggtcat gggcgtggag atgatcaatc aaaaaatggc
    28441 ggcaatcgat gactttcccg cgaggctcgc gctggaactg cgccacatca tcctgagcca
    28501 tcacggcgaa tttgaattcg gctcgcccaa acgcccgaaa acccgggaag cgctggtcat
    28561 tcactttatg gatgacctcg acgccaaact gaacgggttc gagagtttca tcgaaggcga
    28621 cgccgccaac ccgggttccg aatggacagc ctacaaccgt ttctttgagc gctatctgta
    28681 taagggaaag tagggggcat ctctgcccgc ctgaagaaac gcaaagaggg cttcccctcg
    28741 cgcgggaaaa gccctccgcg gcaaacgccg tccgaaaagc cctgatttat tttccgtccg
    28801 ctgtttttct actttttcag cgcgctactc ctctctgcaa tttttttatt tatttctcca
    28861 taggatacgg gaattggatt gtcaatgatt taacgaatat cccgccccag atcccggtca
    28921 gaggctcctt gcgcatcgtg cgatcccgcg ccttctgtct tcagggagca ggcagccggc
    28981 aacgcagatt tccgtgcaat tcccgtcgca tggctcgaca tggaccgtaa ccgtcgcggc
    29041 gggatattgc gtcaaaatgg catgcttcac atcacgggcg atgccgtggg aagcctccac
    29101 cgtcatttga ggatcgacct tgatgtgaaa ttcaataaag cgaaagtttc cggctttgcg
    29161 cgtctttaac tgatgataac catggatatc aggtttgtgg ctgacaatca cagagcggat
    29221 ccacctctct tcgtccggtg acaatttcac atccatcaga tcgccgatcg ctttggcgct
    29281 caaatcataa gccgccttca aaataaacac ggcgacaaag attgccgcga ccggatcaag
    29341 ccagtggatg cgcggatcgg caaaaaagaa atggctgacc cagatgacgg ccaggctgag
    29401 catgacgccc acggaggtat agacatcggt tcgcaaatgc caggcatccg ccgtgagagc
    29461 gatggaatcc gcttctttgc cgacggcgaa aagccgccgg gaaacaaaat aattgaggac
    29521 ggcggacaac aacatcacgg caacgcccca gccgggcgat tccacttgct gcgcggacgt
    29581 cagttttcgg accgattcaa aaacaatcca gaatgccgca atgaaaatca gggcggcttc
    29641 gatcaagccg gaaaggcttt cgactttgcc gtggccgaag ggatggacgt cgtcggccgg
    29701 aagatgcgat gtcttgacgg aaaacatggc gatgacggcc gccaggagat ccatcccgga
    29761 atgaatcgcc tcggatagaa tggacacgga gccgatcagc gcgccgacaa aaactttgaa
    29821 aatcaccagg acggcattgg aagcgaccga caacatcgcc acgcgttctt tgcgctgctg
    29881 gatggacgcc aatgataaat ccccctgttt catttttttt gagcatatca cgacggccgg
    29941 ggaatttacc attttttttg atccggacaa tgcggggaat cgctttgcat cgggggaaaa
    30001 ttgtgatacg caaccgccgt aaagagtatt aaaaatcatg cgaaaaaatt ttgtttgccg
    30061 gtttcccgat ggaacttccc gcaactggcg ggattggcgc tggcagttgc gccatcgcgc
    30121 aacatctctg atcgccttga cccggattct ggaaacgccg ccgcgcgacg ggaaaccgct
    30181 ggaacgcgta atgtcccgct atcccatggc cattactccc tactacctgt ctctcctgga
    30241 accggaccat gaaaatgacc ccctgtctct ccaatgcgtg ccggatctca gagagctgtc
    30301 cttctccgcg ggccttaggg acgacccgct cggcgaagac cgctccatgc ctgcgccgaa
    30361 cctgattcag cgctatccgg accgcgtgct ggccatcgtc acacaccgtt gcgccaccta
    30421 ctgccggcat tgcaaccgca aacgtttctg gagcgtccct gaagccggtc ccttaaaaaa
    30481 ccgcctggcg ccaatggtcc gctttgtatc ggaatcgccg cagattcgtg aagtcatcct
    30541 ctccggaggg gacccgctga tgtttgacga cgcctccctg gaatggatcc tgtcatcgtt
    30601 tgcgtccata ccgcacgtcg aggtgctgcg catcggcagc cgcgcccccg tggtcctgcc
    30661 gatgagaatc acgggaaatc tctgccggat tctgaagcgg caccggccgt tatggttcaa
    30721 tacccagttc aatcatcctt cggaaatcac caaagacgcc cagcgggcgt gcaacatgat
    30781 ccaggaagcg ggaattccgg tttccaacca gtcggttctg ctccagggcg tcaacgactc
    30841 gccgaaggtt atgcaaagtc tgctgaacgg attgcagaaa atatcggttc gcccttatta
    30901 tcttttccac tgcgaacccg cgcggggctg caatcatttc cgcacggacg ccaggaaggg
    30961 cctgaatctc atggaaaagc ttcatcgaag ctgctcgggt ctggcgcttc cgcaatacgt
    31021 ggccgatctg cccgggcggg aggggaaagt ccccctttgg cccctgtcgc ggcccatcca
    31081 aaatgccttg cgaaaacatc aagatttttt tgacaatttt gaataattgg attaggaaga
    31141 gcgcgaagat atcgcttcag agccgtcaac gaaggagaaa gactttttat gtacaccgcc
    31201 agtgatttga gaaaaggcct gcgcctgaaa atcgacaacg atccctacgt gatcaccgag
    31261 tttaactttg tcaaaccagg aaaaggacag gcgctttacc gctgcaaatt aaaaaacatg
    31321 ctcaccggaa gccagtttga acggaccttc cgctccgtcg atgttttcga gcccgccgac
    31381 ctgcgcgaaa agaaaatgca gtacctgtac aaggaggggg acaaattctg cttcatggac
    31441 aacgagacct tcgagcaggt ctatctgacc gaggggcagg tcggagacgc cgtaaacttt
    31501 ctgctcgaca atattgaagc tgaaatcctg ctctttgagg accgccccat cggcatcagc
    31561 ctgcccaact tcgtagagct ggtcgtcacc gaggccgaac cgtgggccaa gggcgacacg
    31621 gtgtccggcg cgaccaaacc cgtcaaggtg cagaccggct ataccattct ggtcccgacc
    31681 tttatcagcg aaggcgaaaa aatacgcatc gacacgcgca cgggagaata tctgacgcgg
    31741 gtcaaaggct agccgttttg agtacgctcc ggcaggaaga ttcttttcgc ctggcgcgca
    31801 agtcgcaggc cctgcggctc agagccgaat tcacccaatg ccttcgccgg cgcttcctgg
    31861 agcagggctt tttggaagtg gaaacccccc tgcgcatccc ctcgcccgct cccgaagaac
    31921 atatcgaagc ccttgcgtcg gacggctggt ttttgcagac gtctcccgaa ctctgcatga
    31981 aaaggattct ggccgccggc tatccccgaa tattccagtt ttgcaaatgc tttcgcgcgg
    32041 gcgaaagagg cggcaggcac ctgccggaat tttcgatgct ggagtggtat gccctgcata
    32101 ccgactatca aatgctcatg cgccagtgcg aagcgcttct ccaatccgcc ctcgcggata
    32161 cgggtcattc cgaaaagatc atctggcgga atcaaatcat ccgtctgccg cttccctggg
    32221 agagaatcac cgtgaaggac gctttttccc gatacgcgcc cgtcagcctg caaaacgctc
    32281 tggccggcgg ccggtttgac gaagtcctga ccgaacacgt cgaacccaac ctcggctgtg
    32341 accatccggt gtttctttgc gattatccgg cctccatggc ggcgcttgct aaactgaaag
    32401 agggtgatcc ggccgtcgcg gagcgctttg agctctacat gggcggcatg gaaatcgcca
    32461 acggcttttc ggagctcacc gacgcacagg aacagagacg gcgctttgag gaagccctgg
    32521 aggctcgggc ggcaaaggat ctggcccgtt atgccatgcc ggagcctttc ctggcggcgc
    32581 tcgaaggcct gcccccctgc gccggcatcg cgctgggcat ggaccggata atcatgattc
    32641 tggccgacgc gccgaacatc gacgacgtcg tcgcctttcc gccggaaacc ctgtagccga
    32701 aaaaaaagat gtattgacaa gccttttttt ttcctttagg ttacggagca aacacagaca
    32761 ggatcgcgac acgagaaagt aaataatttt ggagagttat gcatggaaag ccattttcac
    32821 ggttctctgc tgggaatgat tctggatgcg ggattgatgg ttcagtttgt gctgttgatc
    32881 ctgctgattt tttccattat ttcctggacg atcattttca tcaaattccg cacctaccgc
    32941 aggatccggc aggaaaacga ggcctttgac tccgactacc aaagaagcac gaaattgtcc
    33001 gatctcctcc ctgcatccaa aaaatacccc ttctccacga cggcgaaagt gttccgcgtc
    33061 ggctacacgg aaatgaccaa ggcaaaccgg ctctcccggg atgcggcgcg gaccgaagat
    33121 atcagcctgt cctccctgga caacgtggaa cgggcgctca accgcgcctc cagcacggaa
    33181 atgaccaaac tggagtccgc gctgggattt ctggccacaa ccggttcggc cagccccttc
    33241 atcggtctgt tcggaacggt ctggggaatc atggaaacct tcaaatccat cggcgccaga
    33301 ggttcggcga cgctggccgt cgtggccccc ggcatctcgg aagccctgat cgccacggcc
    33361 gctggccttg ccgcggccat tcccgccgtt attttttaca attacttttt gaacaaatcc
    33421 agaacgatgg tgcaggaaat ggataatttc gcggacgaat ttctgaacat cgtcgaacgc
    33481 tatctggtcc gcaagtaagc gaagttcggg tgagacaacg atgcgaaagg cgcgcagaag
    33541 agaatctgaa aaaagatcca tggcggaaat caacgtcacg ccgctcgtgg acgtgatgct
    33601 ggtgctgctg atcatcttca tggtggcggc gccgatgctt tccatgggca ttgacgtcaa
    33661 tctgccgcgc gtcaagtcaa aaagcatcga cctcgccgag gaaaagctga tcctcagcat
    33721 caacgaaaac cgggaaattt acctcaacaa aaccaggctg ccggtgcagg acctgaacgc
    33781 caagctggcg gccattttcg ccgcgcgcat tgaccgggag gtttttctgc gcgccgataa
    33841 aaacgttcct tacggctttg tggtggaggt catgtccgaa atacgcaaag cgggcgtcga
    33901 taaactcggc atgatcaccg agcctccccc cgagggcgca aaatgatgtc cccgctgccc
    33961 ctcaaaaggt atttcggaaa aaacggtagc cccggcaaca tgtttctggc ctcattgttt
    34021 cttcacctcg tgctcatggc cgccgttttc gcgacggttc ccggttcatc aaagccgctg
    34081 acgttcggag ctccttattc cgtttcgctg gtcgggcccg aggtcatcgg acccggcagc
    34141 caggaggcac ctgatcaaaa aagcctttta aaaacaatat catcggatag ctccgtcatt
    34201 ctgaagcgcg aaatcggcgc ctccccgtca tcgtttgtca tgaaaaaaga cgacagcggc
    34261 agatccgaca tcgaaaaagc gatccgcgct cttgagcaga aggaaacagc cgccggtaaa
    34321 aacaaaaatc cggcggcggc ttcaaccccc tccggaaccg ccgcatcgca ggaatatgcg
    34381 tcccagctaa acgcctacag ccgttttgtc tgggccaaag taaaaagaaa ctggtcgctt
    34441 cccgcaaccc tgatgcccaa aaagaatgtc gaagccgtaa tcgaagtgcg aatcgcccaa
    34501 agcggctcgc tggaacacat cggcttcgaa aagcgctccg gcaacagcta ttttgatgaa
    34561 tcggccctcc gcgcggttca aaaatcaacc ccctttccgc cgcccggagg atgggcgtcc
    34621 ggccgtttcg tggaaatcgg catccggttt cattccgcgg agctgcgctg acgcacccgc
    34681 aatcaaggac cgcaaccatg gataaaaaat atcgcctggc cctggcattg atcctcgttt
    34741 ctttgtggat ggtgagcgcg gccgggtttg ccgccggcaa ggtctatatc gacatcgacg
    34801 caccgggttt tcagcagttt cccgtcgcaa ttctggattt ccaaaaccaa aaggcgggga
    34861 cgggagcctc cccgaatgat cccgccgaca gcattcggcg ctatctcgac ctgacgggaa
    34921 tctttcgcgt cctggataaa aaaagcttcc tggaagggat tgagccggga tcacccgagg
    34981 tcatccgctt ttccgactgg tcggcgatcg gcgctgattt tctgctgcgg ggcaatctga
    35041 gggacggagg aaacggcctg tccgcggaaa gccgtctgta tgacgtcacg cgcggccagg
    35101 tgattttcga aaagaaatat tcggccccgg acggcagccc gcagacgctg gggctctcca
    35161 tcgcttcgga cattcttctg gcgctgaccg gggatgcagg agacttccgc acccggatcg
    35221 ctttcgtgat caaaaaaaaa tccggctccg acattttcac catgaattat gacgcttccg
    35281 atctgaaaaa catgacgaat gaccacagca tcgtcgccgc gccccgctgg tcgccggatg
    35341 gccggtattt tgcctttaca tcctataaaa acggccggcc catggtgttt ctgcgccatt
    35401 tgaagaccgg aaaagaaaaa accatcgctt ctttttccgg cctcaatctg tgcggttcct
    35461 tctcgccgga cggcagaaaa atcctgctga cgctgagcaa ggacggcaac gaggaaattt
    35521 atgccctgga tattgaaacg atgcatctga atcgcctgac gaacagtttt tcgatagacg
    35581 tttcccccgc ctggtctccc gacggccgga aaatcgcctt tgtctccaac cgctccggct
    35641 ccccgcagat cttcgtcatg gacgcggacg gaagcaacgt gaagcgggtc actttcgaag
    35701 gcaactacaa cacgtctcca tcctggtctc cccgcggggg aaggatcgcc tatgaaggcc
    35761 tgatcaaccg gaagtatcag attttctccg cggatgagga aggcaacaac ctgattcagc
    35821 tgactttcga tgaagcggac aacgaatacc cttcctggtc tccctccggc aggcagattg
    35881 ttttccactc ccggcaaaac ggcaaaagcc gaatctgcat catgaacgcc aacggaatga
    35941 acctgcgcgt cttgaaggaa agcagggact tgctggtgat gccggcatgg tccccgaggc
    36001 ccggaaaaca gtaagattcc ataattttta ttgacgcgaa gccgcaagtt atgttttaat
    36061 tcgcgcaaat tccgacatac tataaaataa cgaaaggaga acatcatgaa cagacattta
    36121 actgtaatcg gattacttct gatttttgtc ttcagtttaa ccatattcag cggttgcgcg
    36181 gaaaaacagg cggtagtgaa ggatgaggcg atgcaggaac agaaggtggc ggctgcgcag
    36241 gaaggcgctc cggcgcctgt tgcggccgat gacgatgccg ccttgagggc aaaagaggcc
    36301 gcggacaggg aagccgcgct gaaagaacag gcggccaaag acgaagcggc cagaaaagcg
    36361 gccgcccgcg cggcctgggc caaaaagaac gcggaggcgc tggtggatct caacatacag
    36421 aatatttact ttgattacga caaatccggc atccggcccg atgcacgcga aatcctgaaa
    36481 gccaacgccg aaatcttcac caaaaacagc gccgcgaaaa tcattgtgga aggccactgc
    36541 gatgaaagag gaacggccga atacaacatg gccctgggcg aaagacgcgc tcaggaagcc
    36601 aagcagtacc tggtcaatct gggcattgac gcttcccgga ttgaaaccat cagctacggc
    36661 aaggaacgtc cgctggacaa ccgcagcatt gatgaagcct gggcgcagaa cagacgtgcg
    36721 cagtttcttt taaaatagcc gggtctgcca cgggagacat ttttgaaacg cttttggtat
    36781 ctcatactgc cggcaatggt tttttccctt gccgcatgcg cgacctcttc ggatttaaag
    36841 gcgctccgca ccgagctgaa ccagcagatg gaagcaaaat tcgccgcggc ggacacccgg
    36901 ctggatgacc gggtgtccgc cgtgcaaaac gcgcaggcga aaaacactga agacctggcc
    36961 gcgctgcgca aggcaaccgc caacaccggc gtcaacatct ctgaccttcg agatcaggtg
    37021 cagcagctgc ggggtcaggt cgagaccctg aagaaggaaa ccgcccggga ccccaaaaaa
    37081 gacgctgaag ttcaggaaaa gatcgaccag ctgctggtga aagtcaactt catcgagaac
    37141 tttctggaaa tcgggaaaaa agacgcggca ggagccggca acggcgcgac tcccggcaag
    37201 gatcatggaa agcagggcaa ggcggcggtc tatgcggcgg cttactcgac ctttaaaaac
    37261 ggccagtaca cgaaggccag ggaggcgttt caggactttt tgacggcgca tccgacaggc
    37321 gagtattccg acaatgccca gttctggatt ggagagtgct atttttttga aaagaactat
    37381 gaaaaagcca ttctggaata tgaaaaagta accaaaaaat tccccagcag caataaagtg
    37441 ccctacgcgc ttttaaagca gggactctgc tttcaaaatc ttggcgacaa ggtcagcgcc
    37501 aggctccttt tgcagcaggt catcaaggac tatcccaaca ccaaccaggc gagaatcgcc
    37561 cgttccaaac ttcaggaaat caaataggga caaaaccttc cgtctcccgc cgatgatccg
    37621 tggaccgggt ccgtcttgcg tccgccgccg cggcttcaat ccaaacggat gatcttcacg
    37681 ccgtccggaa ccggcagggc aaacaccgac ggatccgctg acggctcaat cttcaaatcg
    37741 tcgtattcca cgcggacgga tgcttcccgg tccgcccatt gaatgaaaat ttttccgggc
    37801 agggcaagcg aaggcccgta gccctcatag cgcaccgaat atatttcctt gccgtccgga
    37861 gcataccgga ccagcctcgt caagggtccg cttttttcca tccagatcgt ttgcgaaggc
    37921 cccgaagggg ttttcatgtc cacgcgcagt ccgccgctct cttcggaatg ccggtacgtc
    37981 acgccggctg cgtccagacg cgggcaggcg ccggaaagaa tcatcaccag gtcttcaaga
    38041 ttcagcgccc aaggcaggaa acgggccatg ttttcagcgg aggggcttcc cgcgtaaaat
    38101 tcattctgcg acgggatcca tattctcatc tctttcggcg tcgcgctgag aaaaaaaccg
    38161 ggcgtgccga tcaccggaag cgcctccagg cggagaaagg aaggcttctg caaaaccaga
    38221 gcggcccgaa ggggataatg cccctccgtc gcggacaggt ccatccgtgc cgtggcgacg
    38281 atcctgtccg tttccccggg ggtccgccgg atcctctccg cggaatccgg agaagaggcg
    38341 ggaccggtaa aagccaggcc ggcggcgcac ccgcacgcca gaagcgacag gagcaacatt
    38401 gccataatcc ccgcggcggg agcatatcca tgacgcatgc ggcatatcct gagcgtgtga
    38461 tacgcggaca aagggttcta gggtttcaaa aggtccatct tgttcttcag gcggctgttg
    38521 gccggatccg ctttgatggc cttccggtac cattccagcg cctcttgccg tcggccggtt
    38581 tcgaaataca cgtctcccag gtgctccatg acgccgggat cctccggcag aaactccagg
    38641 gctttcttca ggtaggtcag cgcgctttcg tagttgcttt tcctgaaatg cacccatccc
    38701 atggaatcca gaatgtagcc gctgtccggt ttgatcttca gcgcctgaac gatcatttgc
    38761 tcggcctcgt ccaggtggat cccgcggtcg gcatagctgt agccgataaa gttgagggcg
    38821 tcggcattgc ccggatcgag cgaaagcacc cgcctcatgg attgaatgct ctcctcgaga
    38881 cggcctgttt tctccagcag cgcccccaga agataatgca ggtcggtgtt gccgggaaaa
    38941 agacttatgc cttcccggat tgttttttcc gccgcggcca tgtctttctt ctcttcatac
    39001 agagacgaca gatacaggtg cagcacggcc tgatcttttt tttggccgat ggcccggcgg
    39061 ataatcccga tcgcgcccgc ggaatctcct tccagattgg tcagcctggc cgcctggatc
    39121 tgggcgttgg cgtacagctc aaacttcggg gagatcttct gatactcggt cagggccttt
    39181 tgccgatccc ctttttgctc cagaacgctg gccagcaggt accgcagctt gtcgtcggcg
    39241 ggcgttttct ccaaaagcag cgcaaactcg gcagccgcct cgtcataccg gcgcatatcg
    39301 aagtacaaaa gccccagcgc catgatcact tcccggttgt cggggctgat cttcagcact
    39361 tcccgcagtt ccttttccgc cgcttcctcc cggtttgctt tcatctgcag ctccgcaatc
    39421 tttacgcgga tgccgatccg ggcgggatgg acggccagat agcgccggta aacaccgatg
    39481 gcttcatcat accggttccg gctttcatac agcgcggcaa gtcccgtcca ggccgcctca
    39541 aaggccggcc tttgcgcgat aattttctga tagaggctct ccgcgtccgc cggccggttc
    39601 atctggctca gggttttggc gtaataaaac atcccgatga tgttgtcggg atcgagcgcc
    39661 agcaattccc ggtaggcctt ttcggcctcg gcatatttct tttcctggct gtaaatggtc
    39721 gccagataaa gatacgcgac ggcattgtcc ggatccgttt ccgtgacggt ccggtactcc
    39781 tgaacggctt tgtcgtattc acgcagattg aaatagaggc cgcccatgat ggagcgaagt
    39841 tcgatgttgc ccggctgcct ggccagggtt gcttcgccca gcgccaaagc cggctcgaca
    39901 tcgccgtttt caacgtaaag cgagacgagc tcggtattca aataaggcga atcgggggaa
    39961 aaggaaagcg cgcgcttcat gtgatcgatg gattctgccg gacggccgtc cagcctggac
    40021 aggacagcca gggaatagtg ataggcggcc tctttggaat atgtctccgg agcgggatcg
    40081 ggcgccgccg gacgcgtccg ggcgggaaaa gaagcgcaac cggccaaaag cgcccaaaac
    40141 agcaatccgc agatcaggag aggaatatgc tttttttgca taggagacgt ttgccttcgc
    40201 ttcctgtttt cggagcagca gggaattttc aggatgcccc ttccggcttt gacttcgaaa
    40261 gcagcgccga aaccggaacg atggcgatcc cctgttcccg gaagactttg ccggcctccc
    40321 gaacggcccg aatggtttca ggatacggat ggccgatcac gatcagcggc gagtttccgt
    40381 ccgacgcccg ggcagcctcc atgagaatcc ggtaaatcct ggaatagtcc cgttcattgt
    40441 ccagaaagat ttttctggac gcaactctca gacgggccct ttgcgccgcc gcctgcgtct
    40501 ttgaattccc cgtggtccgg gaatcaataa aaaacagatt tctcttttta atctcacgaa
    40561 aaaccagcgt cagcttctcc tcgtccgcca tgaatttgga acccatgtga ttgttgacgc
    40621 cggaggcata cggcacggac gccatatttc gctccatctg aaagaggatc tcctcgttgt
    40681 tcatatccgt aaaaagagcg ccgctgcccg gcttttcctt cggataggaa agcggctcca
    40741 tgggcagatg cagaagaatt tcgcggccgg cccgatgaag cgtttccgcc gcttcgcgcg
    40801 aatggggaag aagcggcaga acggcaaagg tgatgttcgc gtcaatgtcc agcagttcgc
    40861 gcaccggctt catgtcctgg ccgatgtcgt cgatgataat cgccacctgc cgcccttccg
    40921 tccgggacgg tgcggcttct gtcccgacgc cgctttccgg cggcggcacg gcgtcttttg
    40981 ccgcacgggg ctgtgttttc gcggcggccc ggctttccgc ggcggaaggc tttgcccgct
    41041 tttccgccgc acgcggcggt gcgacttttt ccgtttcttc acggtccgac aggaccatga
    41101 agaagatggc ggcgacggcc aggacgatca gaacgatcag aacggctgcc gcactcttga
    41161 tctttttctt ctttttcatg gccgtcggtg ttgaaccccc ggaatccgca tcctcatttg
    41221 gccaggtttt tcttcatgat atcccagctt ttcaaaatat cgatcgccgt cttgagttga
    41281 ttgtccctgg tcagatcggt cgtttccctg gtgtctccgt tttgattgtc gtcgggcttc
    41341 gccgtatctt ctttggccgg tttgatgtgt cccttgagat ccttttcccg aagcctttcc
    41401 cccggcggat tttccggctc ttccgccggc cgcactagct tgacgacaat gtccggctga
    41461 atgccctcgg cctgaatgga cctgccgttg ggcgtatagt accgggccgt cgtcaatttc
    41521 agcgcggaac cgtcctccag gggaatcacg gtctgcacgg acgccttgcc gaaggtttgc
    41581 gtccccacga tcagcgcccg gccgttgtcc tgcacggcgc ccgccacgat ctccgccgcg
    41641 ctggccgtgc cttcgttcac caggaccacc atgggcacga cgacttcctt gtcgccgctg
    41701 tcgcgcgccg tgtttttggt ttccatcttt ttcgtccggc cgcgggtgga cacaatcacg
    41761 cccgacttca gaaacatgtc ggacacttct atggcctggg tcagcagtcc gcccggatcg
    41821 ttgcgcagat ccagcaccag gcccttcatg ggcttggttt tttcggccac gtcggtaagc
    41881 gctttgcgca ggtcatcggc ggttcgttca tggaaggagg caatccgaat gtagccgata
    41941 tggtcatcaa aagcctttgc cctgacgctt tttacctgaa tcacggcgcg ggtcagcgtg
    42001 atgtccctcg gctgggccat attctcccgc agaatcgtta tggtcacctt ggtgtctttc
    42061 ggaccgcgca gcttcttcac ggcctccata atcgtaatgt cttttgtgga tttaccgtca
    42121 atcttgacga tcgaatcgcc gggtttcacg cccgccttga acgccggtgt gtcctcgatg
    42181 ggggacacta ccgtcagcac gtccttcagg atggtaattt caatgccgat tccgccgaaa
    42241 cgcccctgcg tttccacttc cagctccttg taaagctcag gcgtcataaa cgagctgtgc
    42301 ggatcgagag acttgatcat gccgttgatc gcgccctgaa tcagaaccga agaatcgatt
    42361 tcttccacgt agtttttctg caccatatcc aggacttcat tgaaggtttt aatctccttg
    42421 taaatatttt tgtcccgcgc ggaaaccttg ctgtcggaat acggcccgaa aacgacgaaa
    42481 agcccgaaga ggattacggc caatattggc cacttaccgg aaatattttt catgctctgc
    42541 ctcactgccc gtcgatgatt ttaccacacg ccaagagaat gacttctata gcttcaagta
    42601 tataccaatc ttttttttct tttccatcta tttcggcaag ccgtaaaaga aaataacgga
    42661 gaatcttcag aaaccgcgca aaagcgaatc ggccggccgg gcttctaatc gatgatccag
    42721 gtggtccggc ccgcttcatc cttcaaggca atgctctgca aagccagctc ccggcgaatt
    42781 tcatctgcgc ggccccaatc tttagcgtcg cgggcttttt gccgctcggc aatcagcgcc
    42841 tctacccgat gaatatccat tccgcgtttt tcggcttcga tgtcgcggtc cttcgagaaa
    42901 aagagatccg ggtcggactg aaacacaccc agcgcatcgc caaaatgacg gaaggcctcc
    42961 cgggcggtct ccaggatggc ctgtttgtcg gctggaggca tctttttttc caccgtcatg
    43021 acctggttga cgagccgggc ggcgtcaaac acaaagccca gcgcctgcgc cgtattaaaa
    43081 tcatcgtcca gggcagcgtc gaatttttcc acggatgctc tcaatttggc cgcgaactcc
    43141 cccccgccgg aggcggtttc ggcgccgccc tgatgaacct ccgccgcctg cgcatccttc
    43201 aacaaacgaa gggcggtata gcaccggatc agcgcggtcc gggattcccg gagagaggca
    43261 tccgaataat ccacggggct tcggtaatgg ctctgcagca tgaacagccg cagaacttcc
    43321 ggatggaact gccggacgat ctcccggatc gggaaaatgt ttcccaggga tttggacatt
    43381 ttttccgcat tgatcttcac aaaaccgttg tgcatccagt aatgggccgg aggaactccg
    43441 gttgccgcct gggattgcgc gatttcgttt tcatgatgcg gaaagatcag gtcttcgcct
    43501 cctccatgaa tatcaaaagg ctctcccagg tagcgccggc tcatcgccga acactcgata
    43561 tgccatcccg gcctgcccat tccccacgga ctgtcccagg aaggctcgcc ttccttgctt
    43621 ctcttccaga gcacgaaatc cagcggattt cttttcttgt cgttgacatc cacgcgggcg
    43681 ccggccatca tttcatccag gtttcggccg gacaatccgc cgtatgctga aaatttggcg
    43741 accgcgaaga acacgtctcc gtccaccgcg taggcgatcc ctttttcttc gagagtccgg
    43801 atcagcgcaa tcatgtcctc catgtgcccc gtggctctcg gggtgaccgt cggcgcgagg
    43861 acgttgaggg cggccatatc ttcatcgtgc agcgctatgt agcgttcggc gatctcggca
    43921 atgccgacgc cttcccgatt cgcccgatcg atgattttgt cgtcgatgtc ggtgaaattt
    43981 ttcacatacg tcacgtcgta gccgcgcgcc ttcagatggc ggaaaacgac gtcaaacaca
    44041 accgccgcgc gggcgtggcc gatatggcag acatcgtagg ccgtgatgcc gcacacgtac
    44101 attccgaccg cgccttcgcg gaccggctgg aagatttctt tttttcttgt cttcgtatta
    44161 tagatttttt gggccatttt cttgcctttt tcagggatac agcgggggcg ccccaagacc
    44221 gctgtcttcc ggcagggcgc agaccagatt catgttctgc acggcctggc cggcggcgcc
    44281 cttgaccaga ttgtcgatgg cggaaataat aatcacccgg cgtgtccgcg gatcgacggt
    44341 caggccgatg tcgcagtaat tggatccgca aacggaagaa agattgggaa acgttcccgc
    44401 cgaacaaatc cggatgaatt tttccccggc gtagaaggag gcatacaggg cgtgcagatc
    44461 cgcctgggtc gcttcttttc ggagcgacgc gtacattgtg ctcagaatgc cccttttcac
    44521 cggcagcagg tgcggcgtga aggaaatgaa gaatttttcc ccggccagtt tgttgagctc
    44581 ctgctcgatt tccggggtgt gccggtgctt cccgaccttg taggccttaa agccaccatc
    44641 cgcttcgcag aagagcgatc cgacggccgg gtcccttccc gcgccgctca cgccggaggc
    44701 cgaatcggca atgatcgtgg aaacgtccag gagtttattt ttgagcgcgg gcgcgagccc
    44761 cagaataatg ctggtcggat aacagccggg attggcaatc aacgacgatg ttttaaccgc
    44821 gtcccggtaa agttccggaa gcccgtaaac cgcctgtccg aacaggccgg cgctggaatg
    44881 tttggcatac cactcttcat aggttgccgg atcccgcagg cggtagtcgg cggacagatc
    44941 gatcaccttt ttgcccgctt cgagaaagac gggggcgatc tccatagaca cgccgtgcgg
    45001 cagcgccaga aaaacgacat cgcaaatccc tgcgatttcc cggggcgaag cgtcggtgta
    45061 cgtgagaaac gtcaggccgg caagggacgg aaaaacgtcc gagaccttca cgccggcata
    45121 gcgccttgag gtgacggccg ccagttccac ctgcgggtgt ccggacagaa tccggatcag
    45181 ttcctgaccg gtgtaaccgc tggcgccgaa tatcgccgct tttgttttca taaaaaactc
    45241 caaaaaaaag gggagcccga agcccccctt gaatgattaa cgcttggaaa attggaatct
    45301 ctttcgggcg cccggctggc cgtacttctt acgctccttg atgcgggcgt cgcgcgtcag
    45361 aaatcccgct tttttcagaa tggggcgcaa ctcggcgtcg cattccagaa gcgctttgg
//
LOCUS       DAOPAF010000007        43846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1000_length_43846_cov_53.664223, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000007 DAOPAF010000000
VERSION     DAOPAF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 43846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43846
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_43846_cov_53.664223"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLQ77_01560"
     CDS             <1..476
                     /locus_tag="PLQ77_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09400.1"
                     /translation="VMIGFNAAYAKNYFAKIAPINYILNKESRDQFIERHDGSYPAVR
                     YINGHTQANAKIRLIFLAGRGYHLNRPYDDDASFGMEVIRKLASFSKDEFAFQKYLRS
                     LSCTHLLVRYELFQQFLTDNYSPEEIKRLSGQLRKAVAVVFYDGHYAVLQLNTGG"
     gene            1002..3422
                     /locus_tag="PLQ77_01565"
     CDS             1002..3422
                     /locus_tag="PLQ77_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HPL09401.1"
                     /translation="MSFKIKKAAVLGAGVMGAGIAAHLTNAGIECYLLDIIPFELTED
                     DKKKGLTEKSPAWRNRFAANGLAAVTKSKPASFYSKKNASMVKIGNFEDNLKWLADVD
                     WICEVVVENLKIKQELFAKVEKVVKPTCIVSTNTSGIPIKDISAKFGPALKKRFLGTH
                     FFNPPRYMKLMEIIPGEETDKEVVDFMSKFSEETLGKGVVICKDRPNFVGNRIGVFDL
                     SDCIKIMQEKKMKIDEVDAIVGKALGHPGTAVFGTMDLVGLDVGYHVAKNLYDAVPDD
                     EARDTFKAEPFLDKMIANKWLGNKTKQGYYKKTKDAAGKRVKLMLDIDTMEYVPAGKP
                     KFDSISAAKKLENVADSIKLVFNAEDKAGDFARAYLCKTFIYSANRIGEICDDIMAID
                     NAMKWGYNNKLGPFEAWDAVGVKEAVEVMKKLKLKVPKKIDEMLKKGCESFYATKADG
                     KYYYDFEKKGYAKIDENPKIILLPDFKSRNKIVKENESATLIDIGDGVVCLEFHTKMN
                     SIDEGLNQMLNDSCDIVEKDFEGMVVANHDERAFSAGANVFAVLIAAQKGEWDLLEKS
                     IEALQNGNMRMKYLEKPVVTAPAGLALGGGCEIAMHGARCLPNGETYMGLVEVGVGVI
                     PAGGGCKELLVRLTEGLPDGVVANGSNLQWYMAKAFENIATAKVATSAMEAMELGYIR
                     KSEKINMSRDQQIYEAKQIALSLVMAGYKPPRPALIPVMGENLRGFAEGVLMNMRYGK
                     YISDYDLFVSRKVAYVLSGGDCAEGTYVSEQEILDLEREAFLSLMGQTKTHERIMHML
                     TKGKPLRN"
     gene            3505..4698
                     /locus_tag="PLQ77_01570"
     CDS             3505..4698
                     /locus_tag="PLQ77_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL09402.1"
                     /translation="MSDAVIVEAVRSPGGRYKRGGLAQTRSDAYGIQVVKGLLARVPE
                     VKPEEIDDLIVGCAFPEAEQGMILGRTLAMGAGLPISCSGMTVNRFCSAGIQAIADAT
                     AKIRAGWSDIIIAGGCETMSHIPMGGTAMRPNPDWKFDGSMPNLWVNMGNTAENVAEH
                     YNISREDMDKMGVESNRRAHEAIKAGKFKDEIIPIQAFKYKIKNGKRIRETVTFDTDD
                     GVRWPAKMEDMAKLKSPFRLGGNCTAANSSQMTDGAAFSMLMSAEKAKELKLKPIARL
                     AYFAVAGVAAEEMGVGPAYAIPKVLKMAGLTPKDIDVYEINEAFASQAIYSCRVVGIE
                     DRYWAGDINPNGGAIALGHPLGCTGAKLTAQLLHELKRRGGKRGIVSMCIGGGMGAAG
                     IYEML"
     gene            4872..4946
                     /locus_tag="PLQ77_01575"
     tRNA            4872..4946
                     /locus_tag="PLQ77_01575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4904..4906,aa:Val,seq:cac)
     gene            5001..5375
                     /locus_tag="PLQ77_01580"
     CDS             5001..5375
                     /locus_tag="PLQ77_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPL09403.1"
                     /translation="MKTKQPAKSGKWSRPILFLWGASIGTVIFLSLLPRVDLPVDFWN
                     ADKLYHCAAYGWLALLPAIGFADRRAALSAAFSMIILGLLLEAGQYFIPGRMFSVLDI
                     LANTLGVMLGILPGRFIGKRLE"
     gene            5596..5682
                     /locus_tag="PLQ77_01585"
                     /pseudo
     CDS             5596..5682
                     /locus_tag="PLQ77_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            6137..6880
                     /locus_tag="PLQ77_01590"
     CDS             6137..6880
                     /locus_tag="PLQ77_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL09404.1"
                     /translation="MFKGKRVVVVMPAYNAAQTLKMTYNEVMAQEMVDLVILVDDASS
                     DQTSIIARGLPKTRVHVHEKNSGYGANQKTCYRMALEEGADIVIMVHPDYQYTPKLIP
                     AMASMIGNGLYHCVLGSRILGGHAVKDGMPIWKYIANRFLTLAENMMLGAKLSEYHTG
                     YRAFSRELLEKLPLERNSDDFVFDNQMLAQIIWQGYTIAEVSCPTKYFTEASSINFRR
                     SVQYGIGCLATAVSFRLSKAGLASSPLFS"
     gene            6890..7657
                     /locus_tag="PLQ77_01595"
     CDS             6890..7657
                     /locus_tag="PLQ77_01595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09405.1"
                     /translation="MASKNENRFDDFFENEKYVSLKNHLYNYLLRKRAVEEAMSSEEK
                     GLVLEIGSGISPVFTSWDRVVYSDVSASALKMLQKIHGRGQYVVADAMNLPFEDGLFS
                     HVIASEVLEHLEDDRRALAEIARVIRPGGILIVTFPHRHFYFAHDDRFVRHCRRYELS
                     EMISRLEEAGFYAVEVQKVLGPLEKLTMLAVTGFASVAQKFQRKERASKDNVPPPKFL
                     IRLFQVMNRFYAVLARFDAWIMPRSLSTVLLIRAKKL"
     gene            complement(7839..8372)
                     /locus_tag="PLQ77_01600"
     CDS             complement(7839..8372)
                     /locus_tag="PLQ77_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPL09406.1"
                     /translation="MALFEFEGKKPQVPSDSFVHPAAVLIGDVRLGHECLIAPGVSIR
                     ADFGPVIIGDRSSVQDNSVIHVYPGSESVIGNDVTVAHGAILHDVHIHSRCMIGIGAI
                     ILFNAVCEEDVFVSVGSLVPRGMRVPAGKIVAGNPAQIKRDATEKDKAAARDGIEAYR
                     YLCRQYLATMKPVTGPA"
     gene            complement(8393..9385)
                     /locus_tag="PLQ77_01605"
     CDS             complement(8393..9385)
                     /locus_tag="PLQ77_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPL09407.1"
                     /translation="MSSSRTAISRAREVLKIEAQSLLSLISKVNGNFSRAVDLIYQCK
                     GRVIITGIGKSGLIGRKIVATLTSTGTQALFLHPVEGIHGDLGIVTKKDILLAISNSG
                     ETRELLPIISSVRKIGAPIISFTGVLKSTLAQNSDIVIDVSVEKEACPFGLAPTSSST
                     AALAMGDALAIALIDKRKFQEKDFYKFHPGGSLGARLRATVRDAMITGDRIPRVLSGA
                     AARKAIEVIDRMNVGFVLVTDARDQLLGILTDGDVRRIVSRGSTFEGMTIDQVMTSNP
                     KTIDEKASLAETVEFMQKKEITSLAVVNGKKKLKGYVHLHDIFGRGGSVNISLP"
     gene            9831..10667
                     /locus_tag="PLQ77_01610"
     CDS             9831..10667
                     /locus_tag="PLQ77_01610"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPL09408.1"
                     /translation="MMKAVFLSDAHLKRSTDEPYGLLVRFLTDLGEGRVGPAAAGKEA
                     AGAGTTVDDLYILGDFFDFWFCRPDRIYPEFQPVIRQLVQLRQSGVRIHLFEGNHDFF
                     MREYFYDVLGMDVVEESASIDLGGRRVFAAHGDTLDRTNVFYLTLRKVLRSRTFYHLQ
                     RLIPSPILWAAAGLSSATSKTVTIEDGAVLAEKMYACAVERLFTNHDAVILGHCHRPL
                     LRCSNGEGGKKIFAALGDWIIHRSYLFYEEGKFFMMRYPAGQESAREGFVEKLDISVS
                     SC"
     gene            10705..11550
                     /gene="speB"
                     /locus_tag="PLQ77_01615"
     CDS             10705..11550
                     /gene="speB"
                     /locus_tag="PLQ77_01615"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPL09409.1"
                     /translation="MNYGGIYPECSLRNAKFVVVPVPYDSTSTYQPGSRRGPAAILEA
                     SANMELYDEELKTETFLAGIHTALPVEVDARGPKTMIDAVRKRIRRIVAQEKIPVMLG
                     GEHSITLGAVRAVAEKYPNLTVLQFDAHADLRDRYQKTPYNHACVARRVAEFCDLVQV
                     GIRSMSREEGEFLPESSVKSYSADDVFKHPNWAETVAKSIKGDVYVTIDLDVFDPSVM
                     PATGTPEPGGLFWRDVLGLLRLVSRKCAVRGFDVVELAPLPGVVAPDFMAAKLIYRFM
                     GYLTT"
     gene            11647..11723
                     /locus_tag="PLQ77_01620"
     tRNA            11647..11723
                     /locus_tag="PLQ77_01620"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11681..11683,aa:Met,seq:cat)
     gene            12006..14966
                     /locus_tag="PLQ77_01625"
     CDS             12006..14966
                     /locus_tag="PLQ77_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09410.1"
                     /translation="MADSSNRNRSSKTVPEKKTANSKTSKARSKPVLSPGGARVSPSN
                     DIYHLSKDILSNVGVAIYIVQRGWFVYVSSLYQTMTGYSEKELLHHHCLEFIHPEDRE
                     KTRENAIAALKRERTDPYEYRFIKKNGDVMWILERVASIPYEGTRAALGSFMDITERR
                     RMEMIIRQSEEKYRSILEGIEDGYAELDLKGNLVFFNEALCRIQGYPREELARENYRR
                     LMDEENAAKMFASYHRVFTTGQSEKDVQYEVIAKDGARKSIESSITPVKNDAGRIVAF
                     RGILRDRTGLKQAEEALRRSEERYRTILEESDNAYFEVDLAGNYTFVNDALSRLLGYS
                     KEELIGRTFRDQVNKEDTHLLYRAFGKIYTTGEPERGMVYKSTRKGGETRYSEVSGFP
                     LHNAKGEIVGFRGIGRDITLQRLLEEALRQSEERYRTILEEMDDAYFEVDLAGNYTFV
                     NDAISRLLGYPKEKLLGRTFRDQVDKKDTKILYNAFGKIYTTGKPQRGIFYKYARKDG
                     SARYAEISGFPLHNSKGEIVGFRGIGRDITERKKSEERIQYLATHDGLTGLPNRILFN
                     QLLQRALQSAKRYQRMFAMFFIDLDRFKMINDTLGHDAGDQLLREIAVRFKQKLRAVD
                     TVARLGGDEFVILIEELEDLSYVTTVARRILSAALQPMTIMGEECRVTASIGVSLFPK
                     DGEDEQTLMKNADVAMYFAKEDGKNNFKFYSHDIQSQSARRLSIETRLRFALEKNEFF
                     LQYQARLDIRTGKITGVEALLRWDNPDLGLLMPARFIPVAEDTGMIVPIGRWVLRTAC
                     FQNAAWQKMGLPPVCMSVNLSMRQLNDVHLIVDVERVLRESGLAPHLLELEITEHMIM
                     HNPARIIAVLNNIRKLGVRIAIDDFGTGYSSLAQIRHFPVDALKIDRSFMRNIMENPE
                     DIAVARAILSMGKTLSLTVVAEGVETEEQMAFLQKHACDEMQGFHFSAPVSPEQFADL
                     LRNHLPSSIKSCKE"
     gene            15022..16017
                     /locus_tag="PLQ77_01630"
     CDS             15022..16017
                     /locus_tag="PLQ77_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPL09411.1"
                     /translation="MHNQYTIQASRLFVVVLILAGMVFPVRAAEQKTNISCLGQIIAG
                     ERTIVLSAPEGAIMDRLLVKRGDQVARGAELARLRDYDAQAAAVDCARRDIGLARAGL
                     TLVQKGERPEQVEAQQAVVAAREAALRMHKSKKERYQQLHAKSIVPDDAYEAVLYDFE
                     AAQADLLREKNVLEGMQSGHKEEIRQASAKVAVVEANYRRQMALLEAQRIRAPIAGRI
                     LSIRAYPGEAIGESGILDLADTGKMMILAEIYETDISRVRIGSRAQIRSTVFEGEIGG
                     QVVEIERKVEAGRIYALDPRRHADRRIVMVRIKPDKPERLAPFSNAQVTIILHTR"
     gene            16014..17198
                     /gene="devC"
                     /locus_tag="PLQ77_01635"
     CDS             16014..17198
                     /gene="devC"
                     /locus_tag="PLQ77_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease DevC"
                     /protein_id="HPL09412.1"
                     /translation="MKVFSRIQAWLAPGGISLAWRQLSYDKKRFATAIAGVSFGVIIM
                     LFQQGIYQAFQIMVIRPFVAMQGELALISPDYQYLMTPGHFPERRLYQTISDGDVETV
                     YPVSLSYQKWRNPETGGKIDAAMIGVYAGRNPFIIPEVASREDVLTSPENILFDDGSS
                     PDFGDVAGLFAKQGEVETEINDQRIRVSGIFSMGQTLASYGHVIVGMEAYQRLSGLPS
                     GTINLGMIRLKPGADPEAVAQRLQKIVPEDVLVLTRGQLIKREQVYWQNNTPVGFITI
                     AGMILAMFVGAVIVYQILYTDINDHLKEYATLTAIGLGPGYFVKFILTESLILLVIGF
                     IPGLLITALLFLLAESSAELPTRITLFDTALVFCMAAIMCLIAGFLATRRLRSVDPAD
                     IF"
     gene            17214..18533
                     /locus_tag="PLQ77_01640"
     CDS             17214..18533
                     /locus_tag="PLQ77_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL09413.1"
                     /translation="MATAALHEKALISANDLSHTFGSGEVRLQVLRNVNVDFYPGEIA
                     IIMGPSGSGKTTLLTLAGALRSIQEGSIVIGGIELRGATPDRLLAVRHQIGFIFQAHN
                     LLESLNARENVQMGLVHRTDLTIAEIRSRSMAMLERVGLAGHEHKHPPQLSGGQRQRV
                     AVARALVREPSIIMADEPTAALDKTSGREIVELLQRLAREMRSAVLMVTHDNRILDLA
                     DRILTLEDGNVEENHRGLERVAGLLKEGINRIARYPAFFAGGPGRTPSSDDKAFADET
                     QQMLSRARADIHVLVARRLCVNLVFEAKMLAELSDAASLLEEGVRQFGDLFCARPAAA
                     SVDLVNSLFESLEFLLVTAGEAVSSRKMEDIDMLNRLTEDRGPMMESLRARHFERDEA
                     ASRQVKDYCFDISNLFARLIYFLHVLAGQLAELGREKKNRDRDKKTK"
     gene            18626..19132
                     /locus_tag="PLQ77_01645"
     CDS             18626..19132
                     /locus_tag="PLQ77_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09414.1"
                     /translation="MQVLKFKFGMSGERVDAFGMRFFGDFDSRTGHGGTFSEILSDPD
                     APAMTKHPRVTLWFMFEKIRPYDKVSRLLDQLSAGLKKKGYTIKVSSIDGLVDTTSPD
                     YADKPERRFPASCRMHGCNAAGGFSVTAEKTEAKLKFSSKEVAMIKEMTVKLGQTIYG
                     RSLKMHSL"
     gene            complement(19190..20740)
                     /locus_tag="PLQ77_01650"
     CDS             complement(19190..20740)
                     /locus_tag="PLQ77_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09415.1"
                     /translation="MTSEKLQKEMDQMRKTLNRLQESDAKHREAAEAFRKCEQEYREM
                     VELANSIILRMDTSGKILFVNDYALQFFGFSKEELIGKNLIGTIVPQMESSGRDLAAF
                     MASVCRKPGEHTTNENENMKKDGSRVWILWTNRPVLDEKGQIAEIISVGNDITEKKEA
                     EKILRNARDELEREVRERTKELKKLNEDLLFEVAERKISEEALKESEVRYRSIVENAF
                     EGIFQTTLEGKCTMANAALVSLLGYASAKEYMAATSRPENLYVEASDRQELIGLLKTD
                     GYVKGFETQFYRRDGGKISVSLNVRAMFDERGNFIYYQGTVIDISAEITLRRILDETT
                     GALSTAVEVRDPYTAGHQERVTRLAEAIAAEMNFSDEHISAVHTAGMLHDIGKIYVPA
                     EFLSRPGKITAEEYSILKRHAQEGFEILKNIEYKYPIAEIVYQHHERLDGSGYPRGLR
                     ADQILIEARIISVADVVEAMASPRPYRPALGIEKALNEIRQNRGVLYDENVVDTCLAL
                     FLHKEFYF"
     gene            complement(20811..22118)
                     /locus_tag="PLQ77_01655"
     CDS             complement(20811..22118)
                     /locus_tag="PLQ77_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPL09416.1"
                     /translation="MAGFTHQYREKLTTFEEAAKAVKDGDTLIHGVTIAEPPGLLAAI
                     AHRARLGSLNHLKVYTFNAQKHFAETLGSPDIADVIDSYSWFVSENSRNMVQVGLTQF
                     VPAYLHQVPKIVMETMDVDVAVATVAPMNEDGCFSFGPCNGYMSVMATLCKTLIVEVN
                     ENMPFVYGDALIHISDIEMIVENTVPLLELPPLQPNPLDPVIGKIVAEYIPDRAVIQL
                     GIGTLPNAIAPCLEGHRDLGIHTELLCPGMVDLIQKGVITGAHKNVHAGKHIFSLAYG
                     GKETFEYMNGNKSFENYPSSYVMNPGVIAQNHRMTAVNAILQIDLLGQCNAEFLEGRQ
                     YSGTGGQLDFVRGAYDSPEGKAILVLYSTAKNEKVSRIVSRFPAGTMVTTPRNDAHYV
                     ATEYGIVNLKGKSTRARALALISIAHPKFREGLMREAENMSLM"
     gene            22485..24503
                     /locus_tag="PLQ77_01660"
     CDS             22485..24503
                     /locus_tag="PLQ77_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPL09417.1"
                     /translation="MLDSLIRKYNSRKISTQYLLLLIPAILLLFLAFGFVAYTLGKPE
                     ALKTNYELAEVVAKKTNQALMRWLEEQIRTAQTIARDPRIIAVALSPADPQKRADAQK
                     YLSEMHARHPYYENIPLAAKLAPDRTVAVDVQGEKKIIGNGNFFIDTVGGKTIGKCGP
                     EFSYIKNVFAGKPYFISEVYPSILRGNPIFVVAAPIEHNGEIVGATIVAPRMDYFTEF
                     FVEKSVIGATGYLSMIDQDGMIISHPQKSLILNKKGPALVKPVMDKIAAGESYFLSDF
                     AGTTKAYYVEKLTTAQFHMEDSWYIIFCRNYDEVLSDIFAFLLKLLISMLLVAVAVAL
                     LVYFLTRSLVGKPMEKLTGAADSVAKGDLRVQIDPGSSRNEVGQLSRSINNMTRSIRE
                     QTIRIREAVDTLNATTASIVSASGRQEEAMKSLEGNVAQVGVSSRQISATSQELAGTM
                     ENVKNSAGEASRQANAGQKSLSGLQGAMQSLSAGTDSISEKLALMRQKAASIDSIIAT
                     ITKISDQTNLLSLNAAIEAEKAGEYGRGFSVVAREIGRLANETAISTLDISDIISEMQ
                     ASVADGVSEMDHFRQEVACGVRSAEDATMQLTEVIARVANLGPELEKVNEGMRFQSLG
                     SRQISEAMAELNAGAAQLTGSLSQFKAVTEQLNTAARGLSETVSRVVV"
     gene            24550..25341
                     /locus_tag="PLQ77_01665"
     CDS             24550..25341
                     /locus_tag="PLQ77_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL09418.1"
                     /translation="MDFGLKNKIAVVAAASRGLGRAAARQLALEGATVVICSRRKKEI
                     TQAACEIQEETGSLVVSVRADVTKPKDVRELVSQAGKRFGTVHVLVVNAGGPPAGEML
                     ALTDKDWQKAHELTLMSMVRLTRAVLPMMVRQQWGRIITINSIVAKQPVNELLLSAAI
                     RPGIGGLVKVLANQYAKYNITINTVCPGHIRTQRQEELARFRAEARRISIERYFAETA
                     AAIPAGRLGRPEEIGSVVAFLASEQAAYINGVNLLVDGSAAKGVH"
     gene            25341..26471
                     /locus_tag="PLQ77_01670"
     CDS             25341..26471
                     /locus_tag="PLQ77_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPL09419.1"
                     /translation="MRKKRSSQSRLKKDVLEKSLCTGCGACVGLCPYQAVYKDRTVQL
                     FDCDLPDGKCRAFCPRTPSDLPLLRERLFDASDATDEIGPVKEYWLTRAKDEGLRSRA
                     QHGGTVTALLGLAMSEGWIDAAIVSGRLDGREPEGLLIDRAEELADYAQSRFWAAPTV
                     AAFHRAPESGAGRLGIVATPCQAIALAKIRTAKIDGYRKGGSLSLVIGLFCGWTLALD
                     RFQNLLEKYQLSASALTGMDIPAGKNVLELRTAGGSANVPMDEVDSCVREACRCCVDS
                     TAEFADLSVGAARFGANPEEMRGWNQVIVRSGAGQKLVSLARQRGVLEFREAPPPALV
                     ELKNAAAEKKKKALVRIIAKSRSPKNLLYLNPDDPVVRKYLK"
     gene            26540..28498
                     /locus_tag="PLQ77_01675"
     CDS             26540..28498
                     /locus_tag="PLQ77_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPL09420.1"
                     /translation="MTKLLSPAKSKVLFLGNEAIARGALEAGVSVATGYPGTPSSEVI
                     ESLARVAQERNLYVEWSVNEKVALEVAAAASFAGLRSMAVMKQNGVNVASDFLLHLTA
                     SGTRGGIVLVSCEDPGALSSVNEGESRYFAGMLEMPLLEPADFQEAKDMTKWAFELSE
                     AIGNVVMLRSVTRMSHASGNVICGKFPAKTRKAVFRCDGPIMDSQTGPMISTPVVAKH
                     LLRQQKLKTVQALFEKSPFNCYTGPARPDLLLVTASACSLYAAEAVEMLGLQKRVGIL
                     KIGTTWPLPPRLVSGHLRKAGKILIVEEVMAFLEENVKVLAAGDPKLSKGKTFFGKYD
                     GTLPFAGELNPDIVADALAKIFKIRRDALSPSYLEEARKTAAMLPPREQTFCAGCPHR
                     ASFWSIHNAIALDSREGFVCGDIGCYSMAALTPANGYWTMPTLHSMGSGTGLAGGFAK
                     LAPFGFKKPVLTVCGDSTFYHAVIPALINAQHNRADVTFIVLDNQGTAMTGFQPHPGI
                     EVNALKESVPAVDIAAVCRAIGAKVTVCDPFDLAATGAVLTDFLAEKGLLKVLILRQA
                     CALSPEKKGKKTFEMKVDEELCLGDACGCNRLCTRIFRCPALVWDRKKKKAAIDEVVC
                     AGCGVCASICPQKAIVKTPVASAFASGG"
     gene            28563..29177
                     /locus_tag="PLQ77_01680"
     CDS             28563..29177
                     /locus_tag="PLQ77_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPL09421.1"
                     /translation="MKNKNSGLTKNLILTGVGGQGNVMASRALAGMLVDAGYVVTIGE
                     TFGMSQRGGSVMSHLRISPDAVKSPQIPKGGADVIVALEPLEALRVLTVYGNPGVSLI
                     SNARSIDPMGVITGERDYPSEETIHEAFARLTARCWIFDATAIGLVLGNPVLSNIVMI
                     GALAGTGLLPIDRRMFAKEIRKTLSPEKYALNLKAFDAGCSMIK"
     gene            29244..30455
                     /locus_tag="PLQ77_01685"
     CDS             29244..30455
                     /locus_tag="PLQ77_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPL09422.1"
                     /translation="MTDIYSAIQVSDHVYWVGAIDWSIRDFHGYTTPHGSTYNAYLIL
                     ADKITLIDTVKKPFLEEMLARIASVIDPAKIDYIISNHSEQDHCGCLSEVVGRVRPSK
                     VFASAAGVKNLGEILHNDYVIQAVRDGETLSLGNMELTFMETRMIHWPDSMFTFLAKD
                     GLLFSQDAFGMHLATFERFADELPADMLEYEAATYYANIVLPYSPVVLKALEKVAATG
                     WEIRTIAPDHGPVWRRDLGFILDRYKKWAVQKPANKAVVVYATMWHSTESMAKAIAEA
                     LAQAGVSVKILSLNEAHRSEVIFELLEAGALIVGSSTLNNNMLPQMADVMTYIRGLKP
                     ANLIGAVFGSFGWSGESVKDLETALKGMKVDVVAETVSVRHVPGPEALEKCHELGRTV
                     ASELAKRLSQK"
     gene            30477..30635
                     /locus_tag="PLQ77_01690"
     CDS             30477..30635
                     /locus_tag="PLQ77_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPL09423.1"
                     /translation="MKKYVCDVCGYVYDPDQGDEEGGIPPGTPFEKLPADWTCPVCGA
                     DKSEFKPE"
     gene            30662..31162
                     /locus_tag="PLQ77_01695"
     CDS             30662..31162
                     /locus_tag="PLQ77_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPL09424.1"
                     /translation="MDEKIREALREAYVGEAKAVLRLKMFAEKADQEGYPQIARLFRV
                     IAFSEEIHGKRALRVLKEIKTTEENLAESFESEKTVAEIAYDRFVKMAEQAGDKQALL
                     HFSQSRDVEETHAKLYKQAMNHFMEERQTVYHVCKVCGYVADGVLPEECPVCAAKKDQ
                     FTTFDR"
     gene            31178..32026
                     /locus_tag="PLQ77_01700"
     CDS             31178..32026
                     /locus_tag="PLQ77_01700"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HPL09425.1"
                     /translation="MAYGRIFPVLLAFVLLILPIKAAGAEKPLWEVGVGGGFLMMPDY
                     RGSDKTRPYLLPFPYAVYRGGIFRLEDKRLTARLFKTDRVTLDASGYGAVPVKSDDNE
                     ARRGMEDLDPTFEFGPALRIKLLDSRENWHRLSLAFPVRAVFSTDFRSVRYEGWVFSP
                     RLNFEKGNVIPETGIYLGVSAGPMFGDRGCHQYFYNVEPPYAAADRPAYDAGGGYSGS
                     TLTVGLGKNYRSFHFHAFVSADFLQGAVFEDSPLVKTKTSWMSGFSVTWIFLKSKKTV
                     ADDTPY"
     gene            32035..32469
                     /locus_tag="PLQ77_01705"
     CDS             32035..32469
                     /locus_tag="PLQ77_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPL09426.1"
                     /translation="MAAGQGSFITVLLIRWLVITAAIVLASSLLPGIHVASLATAFLA
                     AAILGVLNTFLRPVLLLLTLPLTILTLGIFAFVLNALMLILVAYFVPGLEIDGFFWAF
                     LGALIVSIASWLANRFIYQQQAAPRGKPDVIDLERGDDGKWR"
     gene            32460..35084
                     /gene="mutS"
                     /locus_tag="PLQ77_01710"
     CDS             32460..35084
                     /gene="mutS"
                     /locus_tag="PLQ77_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPL09427.1"
                     /translation="MALNATFNLTPAMKQYVEIKEKYPDCILFYRMGDFYEMFFEDAQ
                     RAAPLLEITLTSRNKSSQDAVPLCGVPYHAASAYITRLVEKGYKVAVCEQVEDPKNAK
                     GIVRREVVRVITPGLVLDEENLKASENNYLACLAAGPDALGLAFLDLSTGEFYISEFP
                     GRDALLAASGPLAFRELLVDRNFPDPALVKMLEARMPSVCVNTIDAAGFEPGQADEIL
                     EKELDAAVLEKSGIRGFRAAKNAAAGILRYVNATQRVRAGHISAIREYAPRNYMMLDE
                     AARRNLEIFATLQDGRSAGSLFHLINETLTPMGARRLRWWMGYPLTAPEKIRERLAAV
                     AEIRESHAARAQLRKILSGVYDLERLAGRIALMAATPRDLVALKTSLAVLPSLRSGLE
                     EFTAPALSALRKDMTDMSKTVQLLSRAIVADPPARAQDGGFIAAGFDEELDRLRFVSA
                     DGKQWIAALEARERQTTGIHSLKVGYNSVFGYYIEVTKANAALVPDSYVRKQTLVNAE
                     RYINQELKEFEQTVLHAEEKIRARESELFGALRESLLPDIPVIQYNAFCVAELDAISA
                     LAETAEKHSYVCPEISEDDVIDIRDGRHPVVEAALKKEGFVPNDTLLDLGQNRLLVIT
                     GPNMAGKSTYIRQVALIVILAQMGSFVPASSARIGVADKIFTRIGASDSLAKGQSTFM
                     VEMMETAQILTQATPRSLVILDEVGRGTSTFDGLSIAWAVAEHLHDLAGRGVRTLFAT
                     HYHQLTDLAASRQGAKNYNIAVKEWGEKIIFLRRIMEGGTSRSYGIEVARIASVPADV
                     IARAREILHNLEKGEFDEIGMPRIARGRNVRKPANPQLSLFAEGEDIVLEEIRRLDVS
                     SMTPLEALNILSELKSKL"
     gene            35111..35716
                     /locus_tag="PLQ77_01715"
     CDS             35111..35716
                     /locus_tag="PLQ77_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPL09428.1"
                     /translation="MTTRSFNKRTGILMLAAAVLAAGVLYAPRYLAYSTGGARADAVV
                     VLLGPVFNDRDRYARDLMNRGMADYLVIPAYQKLFSADRGKLKAVFIPKDPDRLKKNP
                     DSAWPPFYEDTHLELIEAKRIMRQYGQKTAIFVSSPYHMRRIRIMVGREFGTSDGHCF
                     LPTPYEKAPVNVWELKASDWRKVGREYLKIVWFLVYSPWVR"
     gene            complement(35721..36566)
                     /gene="rpoH"
                     /locus_tag="PLQ77_01720"
     CDS             complement(35721..36566)
                     /gene="rpoH"
                     /locus_tag="PLQ77_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HPL09429.1"
                     /translation="MKLPVVTGTLDLYIAEINRFPLLTAEEEFKLAMRLKKDNDMEAA
                     EKLIVSNLRFVVKIAHEYRSYGFKLSDLVQEGNIGLIHAVKKFDPEKGYRLISYAVWW
                     IRAYIQNYLIKSWSLVKIGTTQAQRKLFFKLSQARKQLETISKKNPEFSEIADSLGVK
                     GSEVAEMDLRMGARDLSLNELINDDGETSHLDFLTHDGDNQEVSLIKHEERSLVKRHI
                     AGALARLNERESYIIQHRVMADDPLTLQEIGDKYNITRERARQIEKQALKKVQLALPY
                     LKEDL"
     gene            36711..37589
                     /locus_tag="PLQ77_01725"
     CDS             36711..37589
                     /locus_tag="PLQ77_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPL09430.1"
                     /translation="MNMKSVNKTMIETAIKLAKGVKAGCVLVCVDVRSELAELSEEER
                     KSVRFVLVLREDEELPEKLLPAAKKLELPDVNLTRVGKIKIAIAKGIVSGLFKKGDKV
                     VCLSGVPKFGYADSIFFIDVGREFEILTSDDISDVVEAVRPEVFNAVLNIACELAAQG
                     RENRKVGTIFVLGDDEKVMQLSRQMIINPFKGYSEEQRNILNPELEETIKELSAIDGA
                     FVIKTDGVIVTAGRHLNAALDSKDFPSGLGSRHIAAAGITNVTRAVAMVISQSSGNVS
                     VFKNGKLFVTIEKPVE"
     gene            37603..40191
                     /gene="clpB"
                     /locus_tag="PLQ77_01730"
     CDS             37603..40191
                     /gene="clpB"
                     /locus_tag="PLQ77_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPL09431.1"
                     /translation="MRFDKFTLKVQEALQDAQTLAGTLGHQAIEPEHLLASLLQQQDG
                     IAGAILNKLNVSPRKVEQELHIDLKKQPGVEGAGAGQAYLGGRLNKLLDKAMTGALQL
                     KDEYVSAEHVLVAMSEEKEGKTGKILRQNGVTKDNILKVLVDIRGNQRVTDPNPEGKY
                     QALERYAKDFNELARTGKFDPVIGRDEEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKSKRVIGLDVGALIAGAKYRGEFEERLKAVLKEVISAEGEII
                     LFIDEIHTVVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYIADRFLPDKA
                     VDLIDEAASRLRIELDSMPSEIDAIDRRIIQLEIEKQSLKKDTDKAALERRDKIEKEL
                     AGLQSEMDRMKMHWSSEKDIIKKIQQIKSQMEALKTEEAGAQREGNLARAAEIRYGKM
                     VELSKALETDQAKLSDVQKNNRMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLIH
                     MDERLQMRVIGQDDGIGAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSGWIQDPSLEEEEK
                     KNRSMEALRATFKPEFLNRIDDIIMFHGLTLEDIHRIVGIQLGLIGRRLAERKLSIEL
                     TEAAKKYIAEEGYSPVYGARPLKRALQKLVLDPLAMDLLAGKFSEGDHIVIGVSKGEI
                     TFSKKG"
     gene            40302..40697
                     /locus_tag="PLQ77_01735"
     CDS             40302..40697
                     /locus_tag="PLQ77_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPL09432.1"
                     /translation="MAEETKGEGFVIKDRRFSEEPDTQTKEEPASAKTEEPKETAGKT
                     SAEPADYPAVNFTNFVISLSTSALFHFGDFPEYEGGKAEKNLPAAKQTIDILDMLQKK
                     TAGNLDPNEAGLIEGVLYELKMRYVKEKG"
     gene            40678..41580
                     /gene="ispE"
                     /locus_tag="PLQ77_01740"
     CDS             40678..41580
                     /gene="ispE"
                     /locus_tag="PLQ77_01740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPL09433.1"
                     /translation="MSKKKAETRRLAPAKINLFLRILRKRPDGYHDLASVMQQISLYD
                     EMTFSLRSKEIVISCPGADLPTDEKNLVYLAARKLFDHAGYSGGVAIELAKQIPLAAG
                     LGGGSSDAAATLLALNDLCSLGLSKSELMKLGAKLGADVPFFMFGPTAFASGIGDRLQ
                     TLENTIKFSMVLINPPFPLSTKSVYEKLNFTLTKKENNYSIPRFYALGDVIRELHNDL
                     EAVSMNMHPELADFKQLLINEGALGALMSGSGPTLFGLFADEESAREARSAIEKKVSG
                     DCRVFFAQSLSGKYEFSPDEELPG"
     gene            41599..41673
                     /locus_tag="PLQ77_01745"
     tRNA            41599..41673
                     /locus_tag="PLQ77_01745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41631..41633,aa:Gln,seq:ttg)
     gene            41699..42640
                     /locus_tag="PLQ77_01750"
     CDS             41699..42640
                     /locus_tag="PLQ77_01750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPL09434.1"
                     /translation="MLERIRVFTGNANRELARKICEKLGVPLGNANVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCTPVNVTLMELLIMIDAMKRASADRITAVVPYYGYARQDRKV
                     APRAPISAKLVADLITTAGANRLLSMDLHAGQIQGFFNIPVDNLFATPVLIDYMKKKY
                     TDDTVVVSPDTGGVERARAFGKRLGASLAIIDKRREGPNEAQVMNIIGNVEGKRVIIL
                     DDMVDTAGTVVQAAKALAEAGALEVSVCCTHPVLSGPAIDRIEKSAIREFIVTDTIPL
                     CERAAGCERIKVLSVSGLLSEAVRRIYYNDSVSSLFI"
     gene            42655..43323
                     /locus_tag="PLQ77_01755"
     CDS             42655..43323
                     /locus_tag="PLQ77_01755"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPL09435.1"
                     /translation="MEITDLAAQVRKEQKKGPARRLRQQGLVPAIFYGKATENIQLAV
                     KSADLFRLHKEKKDHAFIKLIIDDGGSKVEKLSLIKELQTQPLSGKFYHADFYEVDMK
                     KKLAFDVELNFTGRAIGVENGGELQHIKRDIRVLCLPTDLPDHIDVDISAIDIGHSLK
                     VKDIVIPEGLAHLDPPEAAVVAVAAIRVVKAEEAQEAPAEGAEPAEGAAAAEPAKEDK
                     KEKK"
     gene            43381..>43846
                     /gene="pth"
                     /locus_tag="PLQ77_01760"
     CDS             43381..>43846
                     /gene="pth"
                     /locus_tag="PLQ77_01760"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPL09436.1"
                     /translation="MGFHKERGQMVYLVIGLGNPGKRYESTRHNIGFMVVEKLAERWK
                     IELKHKSFNALWGKGAMDKKQVLLAKPQTFMNLSGTAARRLQSFFKTDISNLIVIHDD
                     LDLPFGSIRMKAGGGTAGHKGLSSIESNLGNSGFIRVRLGIGKPVDKSRIEGY"
BASE COUNT         9893 a        11674 c        12805 g         9474 t
ORIGIN      
        1 tcgtcatgat cgggttcaac gcggcctatg ccaagaacta ctttgccaaa atcgcgccga
       61 ttaactacat tctgaacaaa gaatcccggg accagtttat tgaaaggcat gacggcagct
      121 atccggcggt tcggtatatc aatggacata cacaggcaaa tgcaaagatc cgattgattt
      181 ttctggcggg gcgcggctat catctcaaca ggccctatga cgatgacgct tctttcggaa
      241 tggaggttat ccgcaagctt gcgtctttct ccaaggatga gttcgcgttt caaaagtatc
      301 tgcggtcgct tagctgcacg catctgctgg tgcgttatga attgtttcaa cagttcctga
      361 cagacaatta ttcaccggaa gaaatcaagc gattatccgg gcagcttcga aaagcagttg
      421 cggttgtatt ttatgacggt cactacgccg tgcttcagtt gaacaccgga ggatgaagaa
      481 cccgaattca gccaaagtgc gggcggtcag gcaagctgga acaaacagca ggatgaaaag
      541 cctgaattcc cgtcttcatc tgatggtaaa aggcaagtaa atacaattca ttgcaagcta
      601 tctcgctttg ttgcccgatc gtttttaatt tgccttgaca ttaacctatg tttggtttaa
      661 taaccttcgt gacttttagt cagcagtccg atgaaagtca tccctcgggg ttcagcgttt
      721 tcccccctga gtgtccggaa ttgatacccg ggtgtattca cagggcaaaa tcaatttcaa
      781 ctacaattgg atattttctt gaaagtggaa aaggatccac agaatgcctg aaatcactac
      841 caaaacaaat ccgaaagggg acatatgagc cggcaggaaa gcggttcgta aaatattgta
      901 cgcagtaagc aggtgaatat gggtgcgcag cttggcccca aacacattta tgagccgtaa
      961 accaaaatgg cggttcataa ggaaagagaa ggaggagaat aatgtcattt aagatcaaaa
     1021 aagcggctgt tttaggtgcg ggagtcatgg gcgcgggcat cgcggctcat ctgaccaatg
     1081 ccggtatcga gtgctacctg ctcgatatca tcccattcga actgacggaa gatgacaaga
     1141 aaaaaggttt gacggaaaaa agccccgcat ggcggaatcg ctttgcggca aacggtctgg
     1201 ctgccgtcac caagtcaaag cccgccagct tctacagcaa gaagaacgcg tccatggtta
     1261 aaatcggcaa ctttgaggac aatttgaaat ggctggccga tgtggactgg atctgcgagg
     1321 tggtcgtcga gaacctcaag atcaagcagg aactttttgc caaagtggaa aaagtggtca
     1381 aaccgacctg catcgtttcc accaacacct ccggtattcc cattaaagac atcagcgcaa
     1441 agttcggccc cgcgctgaaa aagcgctttc tgggaaccca cttcttcaat cctccccgct
     1501 acatgaaact catggaaatc attcccggcg aggaaaccga taaagaagtt gtggatttca
     1561 tgtcgaagtt cagcgaggaa accctgggca agggcgtggt catctgcaaa gacagaccca
     1621 acttcgtcgg caaccgcatc ggtgtctttg atctgtccga ctgcatcaag atcatgcagg
     1681 aaaagaagat gaagattgat gaagtggacg ccattgtcgg caaggccctc ggccatccgg
     1741 gcacggccgt tttcggcacc atggatctcg tcggcctcga tgtgggatac cacgttgcca
     1801 aaaacctgta tgacgccgtt cccgatgatg aagcccgcga tacgttcaag gccgagcctt
     1861 tcctggataa aatgatcgcg aacaagtggc tgggcaacaa gaccaagcag ggatattaca
     1921 agaaaaccaa ggatgccgcc ggcaagagag tcaaattgat gctggacatc gacaccatgg
     1981 aatatgtgcc ggccggaaag ccgaagttcg attccatttc cgccgcgaaa aagctggaaa
     2041 acgttgcgga ctccattaag ctcgtcttca acgcggaaga caaggccggc gatttcgccc
     2101 gcgcttattt gtgcaaaacc ttcatctatt cggccaaccg gatcggcgaa atctgcgacg
     2161 acattatggc catcgataat gccatgaaat ggggctacaa caacaagctg ggtcccttcg
     2221 aggcgtggga tgccgtcggc gtcaaggaag ccgtcgaagt gatgaagaaa ctgaagctga
     2281 aagtccccaa aaagatcgat gaaatgctga aaaaaggttg tgaatccttc tacgcaacca
     2341 aggcggacgg caaatattac tacgatttcg aaaagaaggg ctatgccaag attgacgaaa
     2401 atcccaaaat catcctgttg ccggatttca agagccgcaa caagattgtc aaagaaaatg
     2461 aaagcgccac cctcattgac atcggcgacg gcgtggtctg cctggaattc cataccaaga
     2521 tgaattcgat tgacgaaggc ttgaatcaga tgctcaacga ttcctgcgat atcgtcgaga
     2581 aagatttcga aggcatggtg gtcgccaatc acgacgagcg ggcattctcc gcgggcgcca
     2641 acgttttcgc ggttctgatc gctgctcaga agggcgagtg ggatctgctg gaaaagagca
     2701 ttgaagccct gcagaacggc aatatgagaa tgaagtatct tgaaaagccc gttgtaaccg
     2761 ctcctgccgg cctggctctg ggcggcggct gcgaaatcgc catgcacggc gcccgctgtc
     2821 tgcccaacgg cgagacctat atgggtctgg tcgaagtcgg tgtcggcgtg attcccgcgg
     2881 gcggcggctg caaggaactt ctggttcgct tgaccgaagg ccttccggat ggcgttgtgg
     2941 caaacgggtc caatctgcag tggtacatgg ctaaagcttt tgaaaacata gccacggcga
     3001 aagtagccac cagcgcgatg gaagccatgg aactgggcta catccgcaag tcggaaaaaa
     3061 tcaacatgag ccgtgatcag cagatctacg aagccaaaca gattgctttg tccctggtga
     3121 tggccggtta caaaccgccc agacccgctt tgattcccgt gatgggagaa aatctccgcg
     3181 gcttcgctga aggcgtactg atgaacatga gatacggcaa atacatttct gattatgatc
     3241 tgttcgtatc cagaaaagtg gcttatgttc tctccggcgg cgactgtgcg gaaggcacct
     3301 atgtttcgga gcaggaaatt ctggatctgg aaagagaagc tttcctgagc ctgatgggac
     3361 agacgaaaac gcacgaacgc atcatgcaca tgctgacaaa aggcaaaccg ttgcgcaact
     3421 aactattatg aaataatgag cgcggcggtt tgaaaaaaag ttcagaaaag cctccgcgcc
     3481 ttctagaaat gaaggaggaa aaccatgagt gatgctgtaa ttgttgaagc cgtaagaagt
     3541 ccaggcggcc gttataaaag aggtggtctt gcacaaacca ggtcggatgc ctatggcatt
     3601 caggtagtca agggccttct ggcgagagtt ccggaagtaa aacccgaaga aatagatgat
     3661 ttgatcgttg gttgtgcgtt tccggaagcc gagcagggca tgattctggg gcggactctg
     3721 gcgatgggag cgggcttgcc catttcatgc tccggcatga ccgtcaaccg tttttgttcg
     3781 gctggtattc aggccattgc cgacgctacg gcaaaaatcc gcgccggctg gtcggatatt
     3841 attattgccg gtggctgcga gaccatgagc catattccca tgggcggtac cgccatgcgt
     3901 ccgaacccgg attggaaatt tgacggcagc atgcccaatc tctgggttaa tatgggcaac
     3961 acggccgaaa atgtagccga acattacaat atttcgcgtg aagatatgga caagatgggt
     4021 gtggaaagca atcgccgcgc tcatgaagcc atcaaggccg gaaagttcaa ggatgaaatc
     4081 attcccattc aggccttcaa atacaagatt aaaaatggca aacgcattcg cgaaaccgtc
     4141 acgtttgaca cggatgacgg tgtgcgttgg cctgcaaaga tggaagacat ggcaaaactg
     4201 aagtcgccct tccggttagg cggaaactgc accgctgcca actcttcaca gatgacagac
     4261 ggcgcagcct tctccatgct gatgtcggcg gaaaaagcga aggaattgaa actgaaaccc
     4321 attgccagac tggcatattt tgcagttgct ggcgttgcgg cggaagaaat gggtgtcggc
     4381 cctgcttacg cgattccgaa ggtcctgaaa atggccggct tgacgccgaa agacattgat
     4441 gtgtatgaaa tcaatgaagc ctttgcgtca caggctattt attcctgccg cgtggtcggt
     4501 atcgaagacc gctattgggc gggtgacatc aatcccaacg gcggcgcgat tgccctcggc
     4561 catccgctgg gatgcaccgg cgcaaaattg accgctcagt tgctgcatga actgaagcgg
     4621 cgcggcggaa aacgcggtat cgtatccatg tgtatcggcg gcggcatggg cgcagccggt
     4681 atttatgaaa tgttgtaatt agaaacccct ccagcgccaa gctgaaaaag tcgctccaac
     4741 ccttccgcca ttgaagcaat gatggcggaa gggcgggcgg catttctttt ttgagccgga
     4801 aaaactcttt catgaaaaaa agtatttttg cttgaaacgg ggggaagatt aggctatagc
     4861 agacggcact tgggcggtta actcagcggg agagtgctac cttcacacgg tagaagtcgc
     4921 tggttcaatc ccagcaccgc ctaccattca aaaatcaggg attatccttt cacggttaat
     4981 cccttttctt tttgaaactg atgaaaacaa aacagccggc aaaatcaggc aaatggtctc
     5041 ggccaatcct ttttctctgg ggagcctcga ttggcacggt catttttcta tcgcttttgc
     5101 cccgggtgga tctgccggtt gatttctgga acgcggacaa actctatcat tgcgccgcgt
     5161 acggatggct ggcgcttctg cctgccatcg gttttgccga tcgccgcgcg gctttgtccg
     5221 ccgctttttc catgatcatt cttgggttgc tcctggaagc cgggcagtat tttattcccg
     5281 gaagaatgtt ttcagttctt gatatccttg ccaacacgct gggagtgatg ctgggcattc
     5341 ttccggggcg cttcatcgga aaacggctgg aatgacaatg atttacggtc aaaatagctt
     5401 gttcgggtga aaatcacctt tttgtgtaaa aaggtttttg cgccttttgt ggagcggctt
     5461 ttttcgatct ttctttgttc ttgaatttat ttgcaatttt tgatttaatc cccatacaaa
     5521 aaaagcgtaa gtcgcatttg cctttggtat attagttgct gaaacaatat gaaaattcag
     5581 acaaggggat gaaaaatgaa taaaaaaggt ttcaccctca tcgagttgat gattgttgtg
     5641 gcgatcatcg gcattctggc ggcaatcgcc atcccgcaat tttccgctta cagagtcaag
     5701 tcgtacaaca ccaaagcaac cgctgaacta agaagcgctt atacggcctg ccaggtttac
     5761 ttttccacaa tggaaggttc gacgacctgc gggattgacg gcatgcatag tcagaaattt
     5821 ttcaattcca gcgacgtcac gctcaacatt acggatgata agctcgatac ctggacggca
     5881 ccggcaaagc ataacgcaag caatgttacc tataccattg acccgaacgg cacggttact
     5941 gttactgcta ctccttaaaa caagattatt tcacgatcct tcgggctttt ccatgaagcc
     6001 cttttctttc caactgaaaa acgttccatc attcatattt catttgtcgc cgacatcatg
     6061 tataaagttc cagcgttatc ctttctggag ggtgagctga ctgatcaata tcaacgcgat
     6121 tgcggagatt cggggaatgt ttaaaggaaa aagagttgtt gtggtcatgc ccgcgtacaa
     6181 cgcggcgcag acgctgaaaa tgacctacaa tgaggtgatg gcacaggaga tggtggacct
     6241 ggtgattctg gtggacgatg ccagttccga tcaaacatcc attattgccc gcggactgcc
     6301 caaaacgagg gttcatgtcc acgaaaagaa cagcggatac ggcgccaatc agaaaacctg
     6361 ctatcggatg gctctggagg agggcgcgga catcgtcatc atggttcacc ccgattatca
     6421 atacacgccg aagctgatcc cggccatggc atccatgatc ggcaacggac tttatcactg
     6481 tgtcctcggc tccagaattc tgggaggcca tgcggtgaag gacgggatgc cgatctggaa
     6541 gtacatcgcc aatcgatttc tcacgcttgc ggaaaatatg atgctgggcg cgaagctctc
     6601 cgagtatcac acgggctacc gcgccttttc acgggaactc ctggagaaat tgccgctgga
     6661 gcggaattcc gacgattttg tttttgacaa tcagatgctg gcccagatta tctggcaggg
     6721 ctataccatt gccgaggtca gttgtccgac caaatacttt accgaagcct cgtccatcaa
     6781 cttcaggcgc agcgtccaat acggcatcgg ctgtctggcg acggccgtga gtttccgcct
     6841 gtccaaagcg ggtctggcca gttcaccgct tttttcttga gaaccggtca tggcatccaa
     6901 aaacgaaaac cgcttcgatg atttttttga aaacgagaaa tatgtttccc tgaaaaatca
     6961 cttgtacaat tacctcttgc gaaaacgcgc cgttgaagag gcgatgagct cggaagaaaa
     7021 aggtctggtc ctggagatcg gcagcggcat ctcgccggtc tttacttcct gggatcgggt
     7081 ggtctattct gatgtgtccg catccgccct gaaaatgctg caaaaaatac atggccgggg
     7141 gcaatacgtt gtcgcggacg cgatgaatct gccttttgag gacggattgt tttcccatgt
     7201 gatcgcctcg gaggttctgg agcatctgga agacgatcgt agggcgctag cggaaatcgc
     7261 tagagttatc aggcctggcg ggattttgat tgtgacgttt ccccatcgtc atttttactt
     7321 tgcgcatgac gatcgtttcg ttcgtcattg ccgccgctac gagctgtccg agatgatttc
     7381 tcggttggag gaagccggat tttacgccgt ggaagttcaa aaggtgcttg ggcctctgga
     7441 aaaactgaca atgctggcgg ttaccggctt cgcctccgtg gcgcagaaat tccaacggaa
     7501 agagcgtgct tccaaagata acgttccgcc cccgaaattc ctgatccgtc tctttcaggt
     7561 gatgaatcgg ttttacgcgg ttttggcccg cttcgacgcc tggatcatgc cccgctcgtt
     7621 atcgactgtc ctgctgatcc gggccaaaaa attataatta gggacagcgc ctcggtattt
     7681 ctgagcttta agatttaagt tttaagggtt aagtaattga cgtctgtttg tagggcgtac
     7741 cttcaggtgc gccaagccaa gttgattttg cacgagggtt tggcgagtct gaagactcgc
     7801 cctgcataaa aaaatggaaa tagggcggtg tccctaattt aggcggggcc cgtgaccggt
     7861 ttcatggtgg ccaggtattg cctgcagagg tagcggtagg cttcgatgcc gtctctggct
     7921 gctgctttgt ccttctccgt tgcgtcccgc ttgatctggg cgggattgcc ggcgacgatt
     7981 ttgccggcgg gcacgcgcat gcctcttgga accagcgatc ccacggaaac aaaaacatct
     8041 tcctcgcaga cggcgttgaa aaggatgatg gcgccgatgc cgatcatgca tcgggaatgg
     8101 atatgcacgt cgtgcagaat ggcgccgtgc gccacggtga cgtcgttgcc gatgacggac
     8161 tcggagccgg gatagacgtg aatgacggaa ttgtcctgca cgctggagcg gtcgccgatg
     8221 atgaccgggc cgaagtccgc gcggatggaa acgcccggtg cgatcaggca ttcgtgcccg
     8281 aggcggacgt cgccgattaa aacggccgcg gggtgaacaa aagaatcgga cggaacctgc
     8341 ggctttttgc cttcaaattc gaacagcgcc atcgccatcc cctttcgttt tgtcagggta
     8401 aagaaatgtt gaccgagccg ccccggccga agatgtcgtg caggtggaca taccctttta
     8461 attttttctt tccgttgacg acggccaggg aagtgatttc ctttttctgc atgaactcga
     8521 cggtttccgc cagcgaagct ttttcatcga tggttttcgg attggaggtc atgacctggt
     8581 caatcgtcat cccttcaaag gtggatcccc ggctgacgat ccggcgcacg tcgccgtcgg
     8641 tcagaattcc aagcagttgg tccctggcat ccgtcaccag cacgaatccg acattcatcc
     8701 ggtcgatcac ttcgatcgcc tttcgcgcgg ctgctccgga gagaacgcgc gggattcgat
     8761 cccccgtaat cattgcgtcc cggacggtcg cccggagcct tgctcccagg gaccctccgg
     8821 gatggaactt gtaaaaatcc ttttcctgaa atttgcgttt gtcgatgagc gcgatggcca
     8881 gcgcgtctcc catggccagc gcggcggtgg agctggaggt cggcgccagg ccgaaagggc
     8941 aggcttcctt ttccacggac acgtcaatga cgatgtcgct gttttgggcc agggtggatt
     9001 tgagaacgcc ggtgaaggag atgatcggcg cgccgatttt tctgacagag ctgatgatcg
     9061 gcagcagctc ccgcgtttcg ccgctgttgg aaatggccag cagaatgtct tttttggtga
     9121 cgatgcccag gtcgccgtga atgccttcca ccggatggag gaaaagcgcc tgtgtgcccg
     9181 tgctggtcag cgtggcgacg attttccgcc cgatcaggcc ggatttgccg atgcccgtga
     9241 tgatgacgcg ccccttgcac tgatagatca gatcgaccgc gcgggaaaag ttcccgttga
     9301 ccttggaaat caggctcaaa aggctctgcg cttctatttt cagaacttct ctggcgcgtg
     9361 atatggcggt tcggctgctg ctcatgtttt tttcctgctt ttgatttctt tcttgcccgt
     9421 tcatccgggc tgatggatag ttagcaaaaa acaccggaca aaacaagaat caattggccg
     9481 cggattttat ttgacaggcg ccggaaaatc catcatggtt gcgcacaaga atgccgattg
     9541 gagaaataat gcagggcggg tcgcttgtga ccttgcgcct cctttaggtg gcggggttat
     9601 caggcctgcc gaacttactt gaaacgatca tatggcgcac ctgaaggtac gccctgcatt
     9661 caaatggtaa aacagcagga cgctgctccc tttgggcagg gtacggtctt gccacccgca
     9721 tgggtggtcg cgagcgttcc ctgcagaaag acgtcaataa cgtaacactt aaaacttaaa
     9781 ttttaaaact tgtaataaag aggcgctgtc cctgtttttt gaaggtccga atgatgaaag
     9841 ccgttttcct gtccgatgct catttgaagc gctcaaccga cgagccctac ggcctgctgg
     9901 ttcgtttcct gacggatctt ggggaaggca gggtagggcc ggccgccgcc ggcaaagaag
     9961 cggccggcgc gggaacgacg gtggatgatt tgtatattct gggcgatttt tttgatttct
    10021 ggttttgccg gcccgaccgg atttacccgg aatttcagcc ggtcatccgc caactggttc
    10081 aattgcggca aagcggtgtg cgcatccatt tgtttgaggg aaaccatgac tttttcatgc
    10141 gggaatattt ttacgatgtg ctgggaatgg acgtcgttga ggaatcggca tcgattgatc
    10201 tgggcggcag acgtgtcttt gccgcccatg gagacacgct ggaccggacc aatgtttttt
    10261 atctcacgct gcggaaagtt ttgcgcagcc ggacttttta tcatctgcag cgcctgattc
    10321 cttcgccgat tctgtgggcg gcggccggcc tcagttccgc gaccagcaaa acggtcacga
    10381 tcgaagacgg agcggttttg gcggaaaaaa tgtatgcctg cgccgtcgaa aggctcttta
    10441 cgaatcatga tgcggtgatt ctagggcatt gccacaggcc gctgctgcgc tgctcaaacg
    10501 gggaaggcgg aaaaaaaata ttcgcggccc tcggcgactg gatcatccac cgctcgtatt
    10561 tattctatga ggaagggaaa ttctttatga tgcgttatcc ggccggtcag gaaagcgcgc
    10621 gtgaaggctt tgttgagaaa ctggacatct ccgtttcttc atgctaggaa accacacttt
    10681 ttttgaggaa aagagggaaa aaccatgaat tacggcggaa tataccccga atgttctctt
    10741 cgcaacgcga agtttgtcgt cgttcccgtt ccctacgatt cgacatccac ctaccagccc
    10801 ggcagccgcc gcggcccggc ggccattctc gaagcgtcgg ccaatatgga gctctacgat
    10861 gaggagctga agacagaaac ctttttggcc ggaatccata ccgccttgcc ggtcgaggtg
    10921 gacgcccgcg gaccgaaaac catgatcgac gccgtgcgca aacgaatccg ccggatcgtc
    10981 gcgcaggaaa agatcccggt catgctgggc ggagaacaca gcatcacgct gggcgcggtg
    11041 cgggcggtcg cggagaaata cccgaatctg acagttctgc aatttgacgc gcatgcggat
    11101 ttgcgcgacc gctatcagaa gacgccctat aatcacgcct gcgtggccag acgggtggcg
    11161 gagttctgcg atctggttca agtgggcatt cgcagcatga gcagggaaga aggagaattc
    11221 cttcccgaaa gcagcgtcaa atcctacagc gccgatgatg ttttcaaaca tccaaactgg
    11281 gccgaaacgg ttgccaaatc cataaagggc gatgtttatg tgacgatcga cctggatgtt
    11341 tttgatcctt cggtcatgcc cgcgaccgga acgcctgagc cgggcggtct tttctggcgc
    11401 gatgttctgg gtttgcttcg gctggtgtcg cgcaaatgcg ccgttcgcgg gtttgacgtg
    11461 gttgaactgg cccctctgcc cggtgtcgtc gcgccggatt tcatggcggc caaactaatc
    11521 tatcgtttca tggggtatct gacgacttag ccgccgcggg ctccattccg cgcgcctgcg
    11581 gccaaataac tccttgacaa accagggaga tgatgtttta aggtcacaaa atttataagt
    11641 tcctgtcgcg gggtggagca gcttggtagc tcgttgggct cataacccaa aggtcgtagg
    11701 ttcaaatcct acccccgcta ccaaaactaa attaagggtt tggcgattct tcgttaaacc
    11761 tttttttacg ccgccatccg gacaccgcgt cggagaagag gtgtctgcag ttccgcgctt
    11821 tttgtccttt tccatccagg cggcgttttt catccgcgcc ggaaagtccg cccatcggcg
    11881 aactggaaaa cctgcatcgg atgcatgctc ttggtgttct tcgtttctgt gttgatgcga
    11941 caactcacgg tgacgctcgg aattcaggat tttgttcatg cgtgggaagc ggaggtcggg
    12001 tgtttatggc ggatagctcc aatcggaacc gttcatccaa aaccgtaccc gaaaagaaga
    12061 cggcaaacag caaaacgtca aaagcccgct ccaaaccggt cctttctccc ggcggcgcac
    12121 gggtttctcc ttccaacgac atctaccatc tttccaaaga cattctttcc aacgtcggcg
    12181 tagcgatcta cattgttcag agaggctggt tcgtgtatgt cagctcgctg taccagacga
    12241 tgacgggcta ttcggagaaa gagctgcttc accaccattg ccttgaattc attcatcctg
    12301 aagacagaga aaagacgagg gaaaacgcaa ttgcggcgtt gaagcgggag cgaaccgatc
    12361 cttatgagta ccggttcatc aagaaaaacg gcgatgtcat gtggattctc gagagggtcg
    12421 cttccattcc ctacgaggga acgcgcgccg cgctgggcag cttcatggat attacagaac
    12481 gcaggcggat ggagatgatc attcgtcaga gcgaagagaa ataccggtcc atcctggaag
    12541 gcatcgaaga cggttacgcg gagctggatc tgaaaggcaa cctggtcttt ttcaacgagg
    12601 cgctgtgccg cattcagggg tacccccggg aagaactggc aagggagaat taccgccggc
    12661 tgatggatga agaaaacgcc gcgaagatgt ttgcctctta tcatcgggtg ttcacgacgg
    12721 ggcaatcgga aaaagatgtc cagtatgaag tcatcgcgaa agacggcgcc cgcaaatcca
    12781 tagaaagttc aatcaccccc gtcaaaaacg atgccggccg gattgtcgct tttcggggca
    12841 tcttgcgcga ccggaccggg ctcaaacagg ccgaagaagc cctgcgccgg tcggaggaac
    12901 ggtaccggac gatcctggaa gagtcggaca acgcctattt tgaggtcgat ctggccggga
    12961 actacacctt tgtgaacgac gccttgagcc gcctgctggg atactcaaag gaagaactga
    13021 tcggccgaac cttccgcgac caggttaata aggaagatac gcatctcttg tatcgcgcct
    13081 tcggcaaaat ttacacgacc ggggagccgg agcgggggat ggtctataag tccacacgca
    13141 aaggcggcga gacgcgctat tctgaagttt ccgggtttcc cctgcacaac gcgaaggggg
    13201 agatcgtcgg ttttcgcgga attggaaggg acatcacgtt gcagaggctt ctggaggaag
    13261 cgctgcgaca gtcggaagag agataccgga ccattctgga agaaatggat gacgcctatt
    13321 ttgaagtgga tctggccgga aattacacct ttgtgaacga tgcgatcagc cgtcttctgg
    13381 gctacccgaa ggaaaaattg ctcggcagaa ctttccgtga tcaggtggat aagaaagaca
    13441 ccaaaattct gtataatgcc ttcggcaaaa tttacacgac cggcaagccg cagagaggca
    13501 ttttttacaa gtatgcgcga aaggacggat cggcgcgcta tgcggaaatt tccgggtttc
    13561 ccctgcacaa ctcgaaaggg gagatcgtcg gtttccgcgg cattgggcga gacatcaccg
    13621 aacgaaagaa atccgaggag cgcatccagt atctggcgac gcacgacggg ttgacggggc
    13681 tgcccaaccg catccttttc aaccagcttc ttcagcgcgc cctccagtcg gcgaagcgct
    13741 atcagagaat gttcgccatg ttcttcatcg atctggatcg cttcaagatg atcaatgaca
    13801 cgctgggcca cgacgcaggc gaccaactgc tccgggaaat cgccgttcgc ttcaaacaga
    13861 agctgcgcgc ggtggacacc gtcgcccgcc tgggcgggga tgagttcgtc attttgatcg
    13921 aagagctcga agatttgagc tatgtcacca ccgtcgcccg aaggattctt tccgccgccc
    13981 ttcagccgat gaccattatg ggcgaggaat gccgcgttac ggccagcatc ggcgtcagcc
    14041 tgttccccaa agacggcgaa gacgaacaaa cgctgatgaa aaacgccgac gtcgccatgt
    14101 atttcgccaa ggaggacggc aaaaacaact tcaagtttta ctcccatgac atccagtcgc
    14161 aatccgccag acgattgtcg atcgagaccc gcttgcgctt tgccctggaa aaaaatgaat
    14221 tttttctgca ataccaggcc cggctggata tccgcaccgg caaaattacc ggagtggagg
    14281 ccctgctgcg gtgggataat cctgatctcg gcctcctgat gccggcgcga ttcattccgg
    14341 tggccgaaga tacgggaatg atcgtgccca tcggccgctg ggttttgaga accgcctgtt
    14401 tccagaatgc ggcctggcaa aaaatggggc ttccccccgt gtgcatgtcc gtcaacctgt
    14461 ccatgcgtca gctcaacgat gtccatctga tcgtggatgt tgagagggtg ctcagggagt
    14521 ccggtctggc gccccatctg ctggagctgg aaattacgga acacatgatc atgcacaatc
    14581 ccgcgcgcat catcgccgtt ctgaacaaca tcagaaaact gggcgtgcgg atcgcgatag
    14641 acgatttcgg aacgggttat tcttctctgg cgcagattcg gcattttccc gtcgatgcat
    14701 tgaaaataga ccggtccttc atgcggaaca tcatggagaa tcccgaagac atcgccgtgg
    14761 ccagggccat cctttccatg ggcaagaccc tcagcctgac ggtcgttgcc gaaggcgtgg
    14821 aaacggaaga acagatggcc ttccttcaga agcatgcctg cgacgaaatg caggggtttc
    14881 acttcagcgc gcccgtctcc cccgaacaat ttgccgatct tctgaggaat catcttccct
    14941 catccataaa atcctgcaag gaataggttt tgcgttcagg attttcttga cagaataaac
    15001 cgagtttctt atggttcgcg catgcataat caatatacga ttcaagcgtc tcggttgttt
    15061 gtcgtggttc tgattctggc cggaatggta tttcccgtcc gggcggctga acaaaaaacg
    15121 aacatatcct gtctggggca gattattgcg ggagagcgga caattgttct gtcggcgccg
    15181 gagggggcga tcatggaccg gctgctggtg aagcgcgggg accaggtggc ccggggggcc
    15241 gaactggccc gattgcggga ctatgatgca caggcggcgg cggtggattg cgccagacgg
    15301 gacattggtc ttgccagggc cggtctgacc cttgtgcaaa aaggagaacg ccccgaacag
    15361 gtcgaggcgc agcaggccgt tgtggccgcc cgtgaagcgg cgctgcgcat gcacaagtcc
    15421 aagaaagagc gttatcagca gctccacgcg aaatcgattg ttccggatga tgcctatgag
    15481 gctgttctgt atgactttga ggccgcccag gcggatttgc tgcgggagaa aaatgttctg
    15541 gaagggatgc agtccggaca caaggaagaa atccgacagg caagcgcgaa agtcgccgtg
    15601 gtcgaagcga actaccggcg tcagatggcc ctgctggagg cgcagcgcat ccgcgctccg
    15661 atcgccggca gaattttgtc cattcgagct taccccggcg aggcgattgg cgagtcggga
    15721 attctggatc ttgccgatac gggaaagatg atgattctgg cggaaatcta tgaaaccgac
    15781 atttcccgtg tgaggattgg aagccgggcg cagattcgtt ccacggtctt cgaaggggag
    15841 ataggcggac aggtggtgga aattgaacgg aaagttgaag cggggcgaat ttatgccctg
    15901 gatccgcgcc gccatgccga ccggcgcatt gtcatggtcc gcatcaaacc ggacaagcct
    15961 gagcgtctgg ccccgttcag caacgcccag gtcactatca tcctgcatac ccgatgaagg
    16021 ttttttcccg cattcaggcc tggctggcgc cgggcggcat ttctctggcc tggcgtcaat
    16081 tgagctacga caaaaaacgc tttgccacgg cgattgccgg tgtttctttc ggcgtaatca
    16141 tcatgctctt tcagcagggc atctatcagg ccttccagat catggtcatt cggccgtttg
    16201 tggcgatgca gggagagctg gcgctgatca gtcctgatta ccagtattta atgacgccgg
    16261 ggcactttcc tgagcggcgc ctttaccaga cgatttccga cggcgatgtc gaaacggttt
    16321 atccggtgtc cctttcctat caaaagtggc gcaacccgga gaccggcggc aagatcgacg
    16381 cggccatgat cggcgtctat gccggcagga atccttttat cattccggaa gtggcttccc
    16441 gcgaagatgt cctgaccagt ccggaaaaca ttttgttcga tgacggatct tcgccggatt
    16501 tcggcgacgt ggccggcctg tttgcaaagc agggggaagt cgagacggaa atcaacgacc
    16561 agcgcattcg ggtcagcggc atcttcagca tgggacagac gctcgcgtcc tacggacacg
    16621 tcattgtggg aatggaagcc tatcagcgtt tgagcggctt gccgtccgga accatcaacc
    16681 tcgggatgat ccgcctcaag cccggggctg atccggaagc ggtggctcag cgtcttcaga
    16741 aaattgttcc ggaagatgtg ctggtgctca cgcgcggaca gctcatcaag cgggagcagg
    16801 tttactggca gaacaacaca ccggtgggtt tcattaccat cgccggcatg atcctggcca
    16861 tgtttgtcgg cgccgtgatc gtctatcaga ttctttacac ggacatcaac gaccatttga
    16921 aggaatacgc caccctgacg gccattggtc tcggaccggg ttatttcgtt aaattcattc
    16981 ttacggagtc cctgattctg ctggtgatcg ggtttattcc cggattgctg atcaccgctc
    17041 ttttgttttt actggccgag tcgtcggcgg aactgccgac ccggattacc ctgttcgaca
    17101 cggcgctggt tttctgcatg gccgcgatca tgtgcctgat cgcgggtttt ttagcgacaa
    17161 gaaggctgcg ttccgttgac ccggccgata ttttttaaag ggaatatgcg cctatggcga
    17221 ctgccgcgct ccacgaaaaa gcgttgattt ccgcgaacga cctgagccac acgtttggca
    17281 gcggcgaggt gcgtcttcag gttctgcgca atgtcaacgt ggatttctac cccggtgaaa
    17341 tcgccatcat catggggccc tccggctccg gaaaaacgac gctgctgaca ctggccggcg
    17401 ctctgcgctc cattcaggag ggcagcattg tcatcggcgg gattgaattg cggggcgcga
    17461 cgcccgacag gctgctggcc gttcgtcatc agattggctt catctttcag gcccacaatc
    17521 tgcttgaatc cttgaatgcc cgggaaaatg ttcaaatggg gctggttcac cgcaccgacc
    17581 tgacgattgc tgaaatccgt tcgcggtcga tggccatgct cgaacgggta ggactggcgg
    17641 gtcatgagca taaacatccg ccgcagcttt ccggcggtca gcggcagcgc gtggccgtcg
    17701 cccgggccct ggttcgggaa ccgtccatta tcatggccga tgaaccgacg gccgctctgg
    17761 acaaaacatc cggccgtgaa atcgtggaac tcctgcagcg tctggcccgg gaaatgcgca
    17821 gcgccgtcct catggtgacg cacgacaacc ggattctcga tctggcggac cggattttga
    17881 ccctggaaga cggcaacgtc gaagaaaatc accgcgggct ggaaagggtt gccggtttgt
    17941 tgaaggaggg catcaaccgc attgcgcgct atccggcttt ttttgccggc gggccggggc
    18001 ggacgccgtc ttcagacgac aaggcttttg cggacgagac ccaacagatg ctgtcccgtg
    18061 cgcgcgcgga cattcacgtt ctggtggccc gccgactctg cgtcaacctg gtgttcgagg
    18121 cgaaaatgct ggcggagctg tccgatgccg caagcctgct ggaggaaggc gtacgccagt
    18181 tcggggacct tttttgcgcg aggcctgccg ccgcttcggt tgatctggtc aactccttat
    18241 ttgaaagcct cgaatttctt ctggtgacgg ccggagaagc ggtttcgtcc aggaagatgg
    18301 aagatatcga catgttgaac cggctcacgg aagatcgcgg cccgatgatg gaatccctgc
    18361 gggcgcgtca ttttgaacgc gacgaagcgg cctcgcgcca ggtaaaggat tattgtttcg
    18421 atatttccaa tctttttgcg cgcctgattt atttccttca tgtgctggcc ggacagttgg
    18481 ccgaacttgg acgggagaaa aaaaaccgtg accgggacaa aaaaacaaaa taaatactgc
    18541 gattggcttc gacatatgat atgtgcgtgc ggcggtggat gaaaaacgaa ggacgccgag
    18601 agattcagac gggaaaagag aggatatgca ggtactaaaa ttcaagttcg gaatgagcgg
    18661 cgagcgggtg gacgctttcg ggatgcgatt tttcggggat ttcgacagca ggacggggca
    18721 cggcggaacc ttcagcgaaa tcctttccga tccggatgcc cccgccatga cgaagcaccc
    18781 aagggttact ctctggttca tgtttgaaaa aatccggccc tacgacaagg tttcccgcct
    18841 gctggatcaa ctgtccgcgg gcctgaagaa gaagggatac acgattaaag tatcatcgat
    18901 cgacgggttg gtggacacca cctcgccgga ttacgccgac aaacctgagc gccgttttcc
    18961 cgcctcctgc cggatgcacg gctgcaacgc agccggcggc ttttccgtga cggccgaaaa
    19021 aaccgaggca aagctgaaat tttcatcaaa ggaagttgcc atgattaaag agatgacggt
    19081 caagcttggc cagaccatct atggccggtc tctcaaaatg cattctctgt aggcggaaag
    19141 cattgccggg tccttcccct tgaaaggcct ttttccaccg gcttcctgtt caaaaataaa
    19201 attctttgtg cagaaacagc gccaggcacg tgtcgacgac gttttcatcg tagaggacgc
    19261 cccggttttg ccggatttca ttcagcgctt tttcgatccc gagcgccggc cggtagggcc
    19321 ggggagaggc catggcctcg acgacatcgg ccaccgagat gattctggcc tcgatcagga
    19381 tctgatccgc gcgaaggccg cggggatatc ccgagccgtc cagcctttcg tggtgctgat
    19441 agacgatttc ggcaatcgga tacttgtact cgatgttttt caggatttcg aagccttctt
    19501 gcgcgtgccg cttcaggatg ctgtactcct cggccgtgat cttgcccggc cggctgagaa
    19561 actccgcggg cacgtaaatt ttcccgatgt catggagcat gcccgccgtg tgaacggcgc
    19621 tgatgtgttc gtcgctgaaa ttcatttcgg cggcgatggc ctcggccagg cgcgtcaccc
    19681 tttcctggtg gcccgcggta taggggtcgc ggacttcaac ggccgtggag agcgcgccgg
    19741 tggtttcgtc cagaatgcgc cgcagggtga tttccgcgct gatgtcgatg acggttccct
    19801 ggtaatagat gaaattgccc cgctcgtcga acatggcgcg gacattcagg gaaacggata
    19861 ttttgccgcc gtccctgcgg tagaattgcg tttcaaaacc cttcacgtac ccgtccgttt
    19921 tcagaagccc gatcagttcc tgccggtcgg acgcctccac ataaagattt tctggacggg
    19981 acgttgcggc catgtattct ttggcggagg cgtagcccag cagggacacc agggcggcat
    20041 tcgccatcgt gcatttgcct tccagggtgg tttgaaaaat tccttcaaag gcgttctcga
    20101 cgatgctgcg gtaccggact tcgctttcct ttaaggcctc ttcggaaatt ttgcgctcgg
    20161 ccacctcgaa cagcagatcc tcattgagct tcttgagttc ctttgtcctt tcccgcacct
    20221 cccgttccag ttcgtcgcgg gcgtttcgca gaattttttc cgcctccttt ttttccgtga
    20281 tgtcgtttcc gacggaaatg atttcggcga tttgtccttt ttcatcgagg acgggccggt
    20341 tggtccacag gatccagacc cggctgccgt cttttttcat gttttcgttt tcgttggtgg
    20401 tgtgttcgcc gggttttcgg cagaccgatg ccatgaaggc cgcaagatcg cgtccggagg
    20461 actccatctg cggaacgatg gttccgatca aattttttcc gatgagctcc tcttttgaaa
    20521 agccgaaaaa ttgaagggcg tagtcgttga cgaagagaat tttgccggaa gtgtccatgc
    20581 gcagaatgat gctgttggcc agctcgacca tttcgcggta ttcctgctcg catttccgga
    20641 aagcctccgc cgcttctctg tgcttcgcgt cggattcctg aaggcggttc agggttttgc
    20701 gcatttggtc catttctttt tgcagttttt ctgaagtcat gatccggtcc tcaacgctcg
    20761 ttggattgac gtccgccggg attcttcttt tagccggcgg gacgggattt tcacatgagc
    20821 gacatatttt cggcttccct catgagccct tcccggaact tgggatgagc gatgctgatc
    20881 agcgccagcg cccttgccct cgtggatttg cctttcaggt tgacgatgcc gtactccgtc
    20941 gcgacgtaat gcgcgtcgtt tcggggcgtg gtgaccatgg ttcccgccgg gaaacggctg
    21001 acgatgcgcg acactttttc attttttgcc gtggaataca aaaccagtat ggctttgcct
    21061 tcgggcgaat cgtaggctcc ccggacaaaa tcaagctgtc cccccgtgcc gctgtactgc
    21121 cgcccttcca ggaattccgc gttgcactgc cccagcagat caatctgaag gatggcgttc
    21181 acggccgtca tgcggtggtt ttgggcgatg acgccgggat tcatgacgta ggagctggga
    21241 taattttcaa agcttttgtt cccgttcata tattcaaacg tctcttttcc gccgtaggcc
    21301 agcgaaaaga tatgtttgcc cgcatgcacg ttcttgtgcg ctccggtaat tacgcctttt
    21361 tgaatcagat ccaccatgcc ggggcacaga agctcggtgt ggatgcccag gtcccggtgt
    21421 ccttccaggc agggcgcgat ggcattggga agggtgccga tgcccagttg aattaccgcc
    21481 cggtccggaa tgtattcggc gacaatcttg ccgatgaccg ggtccagcgg attcggctgc
    21541 aggggcggca attccagcag gggaacggta ttttcaacaa tcatttcgat gtcggagatg
    21601 tgaatcaggg cgtcgccgta aacgaagggc atgttttcgt ttacctcgac gatcagggtt
    21661 ttgcacagcg ttgccatgac ggacatgtag ccgttgcagg ggccgaagct gaagcagccg
    21721 tcttcgttca tcggggcaac cgtggccacg gcaacatcga cgtccatcgt ctccatgacg
    21781 attttcggaa cctgatggag ataggcgggg acgaattgcg tcaacccgac ctggaccatg
    21841 ttgcgggagt tttcgcttac gaaccagctg taggagtcaa tgacgtcggc gatatccgga
    21901 cttcccagtg tttcggcaaa gtgcttctgg gcgttgaacg tgtaaacttt gagatggttc
    21961 agcgagccca ggcgggcgcg atgggcgatg gccgccagca ggcccggcgg ctcggcaatg
    22021 gtgacgccgt ggatcagcgt gtccccgtcc ttgacggctt tcgccgcttc ctcgaacgtc
    22081 gtcaattttt cacggtactg atgcgtaaaa ccggccattt taatattccc gccatccctc
    22141 gacgtgctcg ggatgacaat cctgggtgtc atggtgagcc tgtcgaacca tgacaaggaa
    22201 caagcggcgg cgagcccttc gataaacgca gggcaggtcc cgtcgagcgg tgagtccgtc
    22261 gagcggtgag cccgtcgaac cataccccaa aatccgtccg ccggtccgta tgtttacatg
    22321 gcccgccccg tcctttcaag tgaaatcagc ggccggggcg tgaaaaaaac acagggcaag
    22381 acggccgtcc ggcgcgcgga agttgttgat aaattatcgc caatagggta tccaaatcat
    22441 ccgtaaagga tcaaagaacg aatcacggat gactaagggc gccgatgctg gactcattaa
    22501 tcaggaaata taattcgcgg aaaatttcca cgcagtatct tttgctgctg attccggcca
    22561 tcctgctgct ttttcttgcc ttcggatttg tcgcgtacac cctgggcaaa ccggaagccc
    22621 tgaaaaccaa ttacgagcta gccgaagtcg ttgcgaagaa gaccaatcag gcgctcatgc
    22681 gctggctgga ggagcagatc cgcacggcgc agaccattgc ccgcgacccg agaattattg
    22741 ccgtcgctct ttccccggcg gacccgcaga agagagcgga cgcgcagaaa tacctgtccg
    22801 agatgcacgc gcgccatccc tattatgaaa atattcccct tgccgcaaag cttgccccgg
    22861 accggacggt tgccgtggac gttcagggcg aaaagaaaat catcgggaat gggaatttct
    22921 tcatcgacac ggtgggcggc aaaaccatcg ggaaatgcgg acccgaattc agttacatca
    22981 agaatgtttt tgcggggaag ccttacttca tcagcgaggt atatcccagc atcctcagag
    23041 gaaaccccat ttttgtcgtc gccgcaccga tcgaacacaa cggcgagatt gtgggcgcga
    23101 ccattgtcgc cccgagaatg gattatttca cggagttttt cgttgagaaa tccgtcatcg
    23161 gcgcgaccgg ctacctttcg atgatcgatc aggacggcat gatcatttcc catccgcaaa
    23221 aatctctgat cctgaacaag aaaggcccgg cgctggtgaa acccgtgatg gacaagattg
    23281 ccgccggcga atcctatttt ctgtccgact ttgcgggcac caccaaggcc tactacgtgg
    23341 aaaagctgac cacggcgcag tttcacatgg aggacagctg gtacatcatt ttctgccgga
    23401 actacgatga ggtgctctcc gacattttcg cctttctgct gaagctgctg atcagcatgc
    23461 tcctggtggc cgtcgcggtc gcgctgctcg tctatttcct gacccgaagc ctggtcggca
    23521 aaccgatgga aaagctgacc ggcgcggcgg acagcgtcgc caaaggcgat ctgcgcgtcc
    23581 agatcgatcc cggcagcagc cgcaacgagg tgggccagct cagccgctcc atcaacaaca
    23641 tgacccgaag cattcgggag cagacgatcc gaatccggga ggcggtcgat accctcaacg
    23701 cgaccacggc ctccatcgtt tcggcctcgg gccgccagga agaggcgatg aagagtctgg
    23761 aaggaaacgt ggcgcaggtg ggggtttctt ccagacagat ctcggcgacg tcccaggagc
    23821 tggccggcac catggagaac gtcaaaaatt ccgccggcga agcctcccgg caggccaacg
    23881 ccggtcagaa gagcctttcc ggtctgcagg gcgccatgca aagcctctcc gcgggaacgg
    23941 acagcatttc ggaaaaactc gcgttgatgc gccagaaggc ggcgagcatc gacagcatta
    24001 ttgcgacgat taccaagatt tcagaccaga cgaacctcct gtcgctcaac gccgccatcg
    24061 aagcggaaaa ggcgggggaa tacggaaggg gcttttccgt ggttgcgcgg gaaatcggac
    24121 ggctggccaa tgaaaccgcc atttcaacgc tggatatttc cgacattatc agcgaaatgc
    24181 aggcatcggt cgcggacggc gtgagcgaga tggaccattt tcgccaggag gtcgcctgcg
    24241 gggtccgctc cgccgaagat gcgacgatgc agctcactga ggtgatcgcc cgtgtggcca
    24301 atctcggccc ggaattagaa aaggtcaatg aaggcatgcg cttccagtcc ctgggctccc
    24361 gccagatcag cgaagccatg gccgaactga acgcaggcgc ggcgcagttg accggttctc
    24421 tgtcccagtt taaagccgtc acggagcagt tgaataccgc cgccagagga ctgagcgaga
    24481 ccgtctcccg cgtcgtcgtg taatgcgctt ttgcgttcgt gcttcgcatg aagaaaaccc
    24541 ggagggttta tggattttgg attgaaaaac aaaatagccg tcgtcgccgc ggcaagccgg
    24601 ggactgggca gggcggcggc gcgccagctt gcgctggaag gagcgacggt ggttatctgc
    24661 tcgcggcgca aaaaggaaat cactcaagcg gcctgcgaga ttcaggaaga aaccggttcg
    24721 ctcgtcgttt ccgttcgggc cgatgtcacc aaacccaagg acgttcggga gctggtgtcg
    24781 caagccggaa aacgctttgg gacggtgcat gtgctggtcg taaacgccgg cgggcctccg
    24841 gcaggcgaga tgcttgcgct gaccgacaaa gactggcaaa aggcgcatga gctgacgctg
    24901 atgagcatgg tgcgtctgac ccgcgccgtg ctgcccatga tggtgcgcca gcagtggggc
    24961 aggattatta ccatcaactc gattgtcgcc aagcagcccg tcaatgaact gctgctctcc
    25021 gccgcgatac gccccggcat cggcggcctt gtcaaggtgc tggccaacca gtatgcaaaa
    25081 tacaacatta cgatcaatac cgtgtgcccc ggccacattc gaacccaacg gcaggaggag
    25141 ctggcccgtt tccgagccga agcacggcgg atctccatcg agcggtattt tgccgaaacg
    25201 gccgccgcca tccccgccgg aagactgggc cggccggaag aaatcggaag cgtcgtggcg
    25261 tttctcgcgt cggaacaggc cgcctacatc aacggagtga atctgctggt ggacggaagc
    25321 gcggccaagg gcgttcattg atgagaaaaa aacgaagcag tcagtccagg ctgaaaaaag
    25381 acgttctgga aaaaagcctc tgcaccggct gcggtgcgtg cgtggggctg tgcccttatc
    25441 aggcggttta caaagacaga accgttcagc tttttgactg tgacctgccc gacggcaaat
    25501 gtcgcgcctt ttgcccccga acaccttccg atctgccgct tcttcgggaa agactttttg
    25561 acgcttcaga cgcgaccgac gaaatcggcc cggtgaagga gtattggctc acccgagcga
    25621 aggacgaggg actgcggtcc cgcgcgcagc acggcggaac cgtgacggcg ctcctcgggc
    25681 tggcgatgtc cgaaggatgg atcgacgcgg cgatcgtttc cggccgtctg gatggacgcg
    25741 aacccgaagg ccttctgatc gaccgcgcgg aagagctggc ggattatgct caaagccggt
    25801 tttgggcggc gccgacggtc gccgcgtttc accgcgcgcc ggaaagcggc gcgggcagac
    25861 ttggcatcgt ggccacgccc tgccaggcga tcgcgctggc caaaatcagg acggcgaaaa
    25921 tcgacggata ccgaaagggc gggtcgcttt cgctggtgat cggtttgttt tgcggctgga
    25981 cgctcgccct cgatcgtttt cagaatcttc tggaaaaata tcaactttcc gcgtcagccc
    26041 tgaccggcat ggatattccc gcggggaaaa atgttctcga actccgcacg gccgggggct
    26101 cggcaaatgt gccgatggac gaggtggatt cctgtgtgcg tgaagcctgc cgctgctgcg
    26161 tcgattccac ggcggagttt gccgacctgt ccgtgggcgc cgcccgcttc ggcgcgaatc
    26221 cggaggaaat gcgcggctgg aatcaggtga ttgtccgcag cggtgcggga caaaaactgg
    26281 tttcactggc gcgccaacgg ggcgttctgg aatttcgaga ggcgccgccg ccggcgcttg
    26341 tggaactgaa aaacgcggcg gcggaaaaaa agaaaaaggc gctggtccgc attatcgcca
    26401 aaagccggtc gcccaaaaat ctgctgtatt tgaaccccga cgatccggtg gtaagaaaat
    26461 atttaaagta gggaacagtc gcgccgcccg caggggtggt tcaaaccgtt ttctgcgagt
    26521 gacgagagac gagatgcata tgacaaaatt actctcaccg gcgaaatcaa aggttttgtt
    26581 tctgggaaac gaagccattg cccgcggagc gctggaagcg ggcgtttcgg tggcgaccgg
    26641 ctaccccggc acgccttctt ccgaggtgat cgagtcgctt gcgcgtgtgg cgcaagagcg
    26701 gaatctttac gtggagtggt cggtcaacga gaaggtcgcg ctagaggtgg cggcggccgc
    26761 ttcctttgcg ggcctgcgtt cgatggcggt catgaaacag aacggcgtga acgtggcgtc
    26821 ggactttctg ctgcacctga cggcctcggg aacccgcggc ggcatcgtgc tggtttcctg
    26881 cgaagatccg ggggcgctct ccagcgtcaa cgaaggggag tcgcgctatt ttgccggcat
    26941 gctggagatg ccgcttttgg aaccggcgga ttttcaggaa gccaaggaca tgacgaaatg
    27001 ggcttttgag ctttccgaag caatcggcaa cgtcgtgatg ctgcgctccg tcacccgcat
    27061 gtcgcatgcc agcggaaacg tgatttgcgg aaaattcccg gcgaaaaccc gcaaggcggt
    27121 gttccgctgc gacggaccga tcatggattc gcaaaccggg ccgatgatca gcacgccggt
    27181 ggtggccaaa catctgcttc gccagcaaaa gctcaaaacc gttcaagccc ttttcgaaaa
    27241 aagcccgttc aactgttaca cgggccccgc ccggcctgat ctgctcctcg tcacggccag
    27301 cgcctgttct ctctacgcag ccgaggcggt tgaaatgctg ggccttcaaa agcgggtggg
    27361 cattctgaag atcgggacca cctggcccct gccgcctcgc ctggtgtcgg gccatctgcg
    27421 gaaagccgga aagattctga tcgtcgaaga agtgatggcg tttttggaag aaaacgtcaa
    27481 ggttctggcc gccggagacc cgaagctttc aaagggcaaa acattcttcg gcaagtacga
    27541 cgggacgctt ccttttgcgg gggaactcaa ccccgatatc gtggcggacg ccctggcgaa
    27601 gattttcaag atccggcgtg atgcactttc gccgtcctat ctggaagaag cgcgaaaaac
    27661 ggcggcgatg cttccccccc gggaacagac gttttgcgcc ggctgcccgc accgcgcgtc
    27721 cttctggagc atccacaacg ccattgcgct ggacagccgc gaagggttcg tctgcggcga
    27781 catcggctgc tattcaatgg cggcgctcac gccggccaac ggctactgga cgatgccgac
    27841 gcttcattcc atggggtccg gcacggggct ggccggcggt tttgccaaac tggcgccgtt
    27901 tggattcaaa aaacccgttc tgacggtctg cggcgattcc acattttatc acgcggtgat
    27961 cccggcgctc atcaacgcgc agcacaaccg cgcggacgtc acgttcattg tgctggataa
    28021 tcaggggacg gccatgacgg ggtttcaacc gcatccgggc attgaggtga acgccctcaa
    28081 ggaaagcgtt ccggccgtcg atatcgctgc cgtctgccgg gccatcggcg cgaaggtgac
    28141 ggtttgcgac ccgtttgacc ttgcggcaac cggcgcggtt ctgacggatt ttctggccga
    28201 aaaaggtttg ctcaaagtgc tgattctgcg ccaggcctgc gcgctcagcc ctgaaaaaaa
    28261 gggcaaaaaa acattcgaga tgaaggtgga cgaagagttg tgtctggggg acgcctgcgg
    28321 ctgcaaccgt ctgtgcacaa ggattttccg ctgcccggca ctcgtctggg accggaaaaa
    28381 gaagaaagcg gcgattgacg aggtggtctg cgcaggctgc ggcgtctgcg cttcgatctg
    28441 tccgcagaag gctattgtaa aaacgccggt cgcgtccgct ttcgcttccg gcggatgatt
    28501 ggacggctcc ccgtcccgac ggagcgggga ggagcgtatc gttgtatttt cgggaattgt
    28561 gtatgaaaaa caaaaactcc ggactcacca aaaacctgat tctcaccggc gtgggcggac
    28621 agggcaatgt catggcatcc cgggcattgg cgggcatgct ggtggacgcg ggctatgtcg
    28681 tgaccatcgg cgaaacgttc ggtatgtcgc agcgcggcgg gtcggtcatg agccatctgc
    28741 gcatttcccc cgacgcggtg aaaagtccgc agattcccaa aggcggcgcg gatgtgatcg
    28801 tggcgctgga gccgcttgaa gctcttcggg ttttgacggt gtacgggaat cccggcgtct
    28861 cgctcatctc caatgcccgt tcgatcgatc ccatgggggt gatcacgggc gaacgcgact
    28921 acccttccga ggaaacgatt catgaggcct ttgcgcgatt gacggcccgg tgctggatct
    28981 ttgacgccac ggccataggc cttgtcctgg gcaaccccgt gctcagcaat atcgtcatga
    29041 tcggcgcgct ggccgggacg ggccttttgc ccatcgaccg tcggatgttc gcaaaagaga
    29101 ttcgcaaaac cctttctccg gaaaaatatg ctttaaatct gaaggctttt gacgcgggat
    29161 gctccatgat caaataaggg ttgacgatcc ggcggtaaac cttatattac tccctcacaa
    29221 atcattttcc gaggtgaccc ttcatgaccg acatctattc cgcgatacaa gtgagcgacc
    29281 atgtttactg ggtgggcgcc atcgactggt cgattcgtga ttttcacggc tacaccacgc
    29341 cccacggaag cacgtacaat gcctacctga tcctggcgga taaaatcacg ctgatcgaca
    29401 ccgtcaagaa gcccttcctg gaggaaatgc tggcgcggat tgcatccgtc atcgatccag
    29461 ccaaaatcga ctacattatt tccaatcact ccgaacagga ccattgcgga tgtctctctg
    29521 aagtggtcgg acgggtccgg ccctcaaaag tgtttgcctc cgccgccggc gtgaaaaacc
    29581 tgggagagat tcttcataac gattatgtta ttcaggcggt ccgcgacgga gagacgcttt
    29641 ccctgggcaa tatggagctg accttcatgg aaacgcgaat gattcactgg ccggattcga
    29701 tgtttacgtt tctggctaaa gacggcctgc tgttttcgca ggacgccttc ggcatgcacc
    29761 tggcgacgtt cgagcgcttc gccgacgaac tgccggccga catgttggaa tacgaggccg
    29821 ccacatacta cgccaacatc gttctgcctt attcgccggt agttctcaaa gcgctggaaa
    29881 aggtggcggc gacgggctgg gaaatcagga ccattgcgcc ggatcacgga ccggtgtggc
    29941 gcagggatct ggggttcatc ctcgaccggt ataagaaatg ggctgtgcag aagccggcca
    30001 acaaggcggt ggtggtttat gcgaccatgt ggcattcgac cgaaagcatg gccaaagcca
    30061 ttgcggaggc cctggcgcag gcgggtgtgt ccgttaaaat tctttccctc aacgaagcgc
    30121 accgcagcga ggttattttt gaactgctgg aggcgggggc gctgatcgtt ggttcgtcca
    30181 cgctcaacaa caatatgctg ccgcagatgg cggatgtcat gacctatatc cgggggctca
    30241 aaccggccaa tctgatcggc gcggtgttcg gctctttcgg ctggagcgga gagtcggtga
    30301 aagatctgga aacggccctc aaaggaatga aagtggacgt ggtggccgaa acggtttccg
    30361 tccggcatgt gccgggtccg gaagcgcttg aaaaatgcca tgagctgggt cggaccgttg
    30421 cgtcggaact ggccaaaaga ctgtcccaga aataagacca ttcaaggagg cgaatcatga
    30481 aaaagtatgt gtgcgatgta tgcgggtatg tttatgatcc ggaccaggga gacgaagaag
    30541 gtggaattcc ccccggaacg ccgtttgaga agcttcccgc ggactggacc tgtccggttt
    30601 gcggcgccga taaaagcgag ttcaagccgg aataagtatt tttcaggaaa gggggtaagc
    30661 gatggatgaa aagattcggg aagcgcttcg agaggcctac gtcggcgagg cgaaagccgt
    30721 cctgcggctg aaaatgttcg cggaaaaagc cgatcaggaa ggctatccgc agatcgcgcg
    30781 cctttttcgc gtgattgctt tttcggaaga gattcacggc aaacgggcgc tgcgcgtgct
    30841 caaggagatc aaaacgacgg aagaaaacct tgcggaaagc ttcgagtcgg aaaaaacggt
    30901 ggcggaaatc gcctatgacc gctttgtaaa gatggcggag caggcgggcg acaagcaggc
    30961 ccttctgcac ttcagccaga gccgcgacgt ggaggaaaca cacgccaagc tctacaaaca
    31021 ggccatgaac cacttcatgg aagagcgcca aaccgtttat catgtctgca aggtctgcgg
    31081 ctacgtggcg gacggcgtat tgccggagga atgtccggtt tgcgcggcga aaaaagacca
    31141 gtttacgact tttgaccgct aggattgggc gttcgtgatg gcgtatggtc gaattttccc
    31201 agtgctgctg gcttttgttt tgctgatcct gccgatcaag gctgccggcg cggagaagcc
    31261 tctgtgggaa gtgggagtcg gcggcggttt tctgatgatg ccggattacc ggggatcgga
    31321 caagacgcgg ccgtacctgc tgccgtttcc ctatgcggtt taccggggcg gcatttttcg
    31381 cctggaggac aaaaggctga ccgccaggct tttcaaaacc gaccgcgtaa ccctcgatgc
    31441 gagcggctac ggggccgtgc ccgtgaagag cgacgacaat gaagcgcgcc gcggcatgga
    31501 ggacctggat cccacgtttg aattcggccc ggcgctgcgc ataaagcttt tggactcccg
    31561 ggaaaactgg catcgcctga gcctggcctt tcccgtccgg gcggtttttt cgacggactt
    31621 tcgctccgtg cgctacgagg gatgggtctt ttccccccgg ttgaactttg aaaaaggcaa
    31681 tgtgattccc gaaacgggca tctacctggg agtttcggcc gggccgatgt tcggcgaccg
    31741 aggctgccat caatattttt acaacgtgga acccccttac gccgcggctg accgcccggc
    31801 ctacgatgcg ggcggaggct acagcggctc caccctgacc gtcgggctgg ggaagaatta
    31861 caggtccttt cattttcacg ccttcgtcag cgccgatttt ctgcagggcg ccgtttttga
    31921 ggacagcccg ctggtcaaga cgaaaacatc ctggatgagc gggttcagcg tcacgtggat
    31981 ctttctgaaa tcgaaaaaaa ccgtggcgga cgatacgcct tactgaggag gaatatggca
    32041 gcgggccagg gatcattcat aacggttctt ctcatacggt ggctggtcat taccgcggcc
    32101 attgtgcttg cgtcgagcct ccttcccggc attcacgtgg cgtctctggc gacggccttt
    32161 ctcgccgccg cgatcctggg cgttctgaat acttttcttc gccccgtcct gctccttctg
    32221 acgctgccgc tgaccattct gacgctgggc atctttgctt ttgtgctcaa cgctctcatg
    32281 ctgattctgg tggcttattt cgtcccggga ctggaaattg acggtttttt ctgggcgttt
    32341 ctcggcgccc tgatcgtcag catcgccagt tggctcgcca accgttttat ctatcagcaa
    32401 caggccgctc ctcgcgggaa gcccgacgtg attgatctgg agcgggggga tgacgggaaa
    32461 tggcgctgaa cgcgacgttc aatctgaccc cggcgatgaa gcagtacgtc gagatcaagg
    32521 aaaaatatcc ggactgcatt ttgttttacc gcatgggcga tttctacgag atgtttttcg
    32581 aagacgcgca gagggccgcg ccgcttctgg aaatcacgct cacctcccgc aataaatcca
    32641 gccaagacgc ggtcccgctt tgcggagttc cctatcacgc cgcttcggcc tacatcacaa
    32701 ggcttgtgga aaaaggatac aaggtggccg tctgcgagca ggtggaggac ccgaaaaacg
    32761 ccaaaggcat cgtccggcgc gaagtcgtcc gcgtgatcac gccggggctg gttctggacg
    32821 aggaaaacct gaaagcctcc gaaaacaact atctggcctg cctggccgcc ggccccgacg
    32881 cgctgggcct ggcgtttctg gatttgtcca ccggcgagtt ttacatctcc gaatttccgg
    32941 gacgcgacgc gcttctcgcc gcctcggggc cgctggcctt tcgggagctg ctggtggaca
    33001 gaaattttcc cgatccggcg ctcgtcaaaa tgctggaggc ccgaatgccg tccgtttgcg
    33061 tcaacacgat cgatgcggcc ggtttcgagc ccggacaggc ggatgagatt ctggagaagg
    33121 agctcgatgc ggcggtgctg gaaaaatcag gcatccgggg ctttcgcgcg gcgaaaaacg
    33181 ccgccgccgg aattctgcgc tacgtgaacg ccacgcagag ggttcgcgcc ggccatattt
    33241 ccgccattcg ggaatacgcg ccccggaatt acatgatgct cgatgaggcc gcccgccgaa
    33301 acctggaaat cttcgccacg cttcaggacg gaagaagcgc gggatccctt ttccacctca
    33361 tcaacgaaac cctgacgccg atgggcgccc ggcgcctgcg gtggtggatg ggctatccgc
    33421 ttacggcgcc ggaaaaaatc cgcgagcggc tggcggccgt ggcggaaatc agggaaagcc
    33481 acgcggcgcg cgcacagctt cgcaaaattc tttccggcgt ctatgacctg gaaagactgg
    33541 ccggacgcat cgcgctcatg gcggccacgc cgcgcgacct cgtcgcgctg aaaacgtctc
    33601 ttgccgtttt gcccagcctg cgatccgggc tggaagaatt taccgcgccc gctctcagtg
    33661 ccttgcgcaa agacatgacg gacatgtcca agacggttca actcctttcc cgcgcgatcg
    33721 tcgccgatcc tcccgcccgc gcgcaggacg ggggatttat cgccgcgggc tttgatgagg
    33781 agctggatcg cctgcgcttt gtcagcgccg acggcaagca gtggatcgcg gcgctcgaag
    33841 cccgggagcg tcagaccacg ggcatccatt cgctcaaagt cggctacaac agcgttttcg
    33901 gctactatat tgaagtgacc aaggccaacg ccgcgctggt gccggactcc tacgtgcgca
    33961 agcagacgct ggtcaacgcg gagcgctaca tcaaccagga attgaaagag ttcgaacaga
    34021 cggtgctcca cgccgaggaa aaaatccgcg cgcgggaaag tgaactcttc ggcgcgcttc
    34081 gcgaaagcct gctgccggac attcccgtca ttcagtacaa cgccttttgc gtggccgaac
    34141 tggatgcgat ctcggctctt gcggaaacgg cagagaagca ttcctatgtc tgcccggaaa
    34201 tcagcgaaga cgacgtgatc gacattcggg acggccgcca tccggtggtc gaagcagccc
    34261 tcaaaaagga aggcttcgtg cccaatgaca cgctgctcga tctcggccaa aaccgtctgc
    34321 tggtcattac cggacccaac atggcgggaa agtccacgta tatccgccag gtggccctga
    34381 tcgtgattct ggcgcaaatg ggcagtttcg ttcccgcctc cagcgcccgc atcggcgtgg
    34441 ccgacaaaat cttcacgcgc atcggcgcct cggacagcct ggccaaaggc cagagcacgt
    34501 ttatggtgga gatgatggaa acggctcaga ttctcactca ggcaacgccc cgcagcctgg
    34561 tcattctgga cgaggtgggc cggggcacat ccacgtttga cggcctcagc atcgcctggg
    34621 cggtggccga gcatcttcac gatctcgccg ggcggggcgt gcgcacgctg tttgccacgc
    34681 actaccacca gttgaccgac ctggccgcgt ccaggcaggg cgcgaaaaac tacaacattg
    34741 ccgtcaagga atggggggaa aaaatcattt tcctgcgccg gatcatggag ggggggacga
    34801 gccgcagcta cggcatcgaa gtggcgagaa tcgcctccgt cccggcggat gtcattgccc
    34861 gggccaggga aatcctgcac aatctggaga agggggaatt cgacgaaatc ggcatgccgc
    34921 ggatcgcccg cggccggaat gtccgaaagc cggccaaccc gcagttgagt ctgttcgcgg
    34981 agggcgagga catcgtcctt gaagaaatcc gccggctgga cgtttcttcg atgacgccgc
    35041 tggaggcgct caacatcctc agcgagctga aaagcaaatt atgaccggga aaacaaaaag
    35101 aaatcggccc atgaccacgc ggtcctttaa taagcgaacc ggcatactga tgctggcggc
    35161 ggccgttctc gcggcagggg tgctttacgc gccccggtat ctggcgtatt cgaccggcgg
    35221 cgccagggcg gatgccgtcg tggttctgct ggggcccgtt ttcaacgacc gggaccgcta
    35281 cgccagggat ttgatgaacc ggggaatggc ggactatctc gtgattcccg cctatcagaa
    35341 gctcttcagc gctgaccggg gaaaactgaa ggccgttttc attccaaaag acccggaccg
    35401 cctcaagaaa aaccccgact ccgcctggcc gccattttac gaagacactc acctggagct
    35461 catcgaggcg aaaagaatca tgcgtcaata cgggcagaaa acggcgattt tcgtgagctc
    35521 tccctaccac atgcggcgca tccgcattat ggtcggcagg gaattcggaa cgtccgacgg
    35581 acattgcttt ttgccgacgc cctacgaaaa agccccggtg aatgtctggg agcttaaggc
    35641 ctcggactgg agaaaagtgg gcagggaata cctgaagatc gtatggtttc tcgtttattc
    35701 cccgtgggtc cgctgacggc ttacaggtct tctttgagat agggcagggc cagctgaacc
    35761 tttttgagcg cctgcttttc aatctggcgg gcgcgctcgc gggtgatgtt gtatttgtcg
    35821 ccgatttcct gcagggtcag cggatcatcg gccatgacgc ggtgctgaat gatgtagctt
    35881 tccctttcgt ttagcctggc cagggcgccg gcgatgtgcc gtttgaccag gcttctctct
    35941 tcatgcttga tcagggagac ttcctggttg tccccgtcat gggtcagaaa atccaggtgg
    36001 gacgtttcgc cgtcgtcgtt gatgagttcg ttgagcgaca ggtcgcgcgc gcccatccgc
    36061 agatccattt ccgcgacctc ggagcccttg acgcccagcg agtcggcgat ctcgctgaat
    36121 tcgggattct tcttcgagat cgtttccagc tgtttccggg cctggctcag tttgaagaaa
    36181 agcttgcgct gcgcctgtgt ggttccgatc ttgacgaggc tccaggattt gatcaaatag
    36241 ttctgaatat aggcgcggat ccaccagacc gcgtaggaaa tcaggcggta gcctttctcc
    36301 gggtcgaatt tcttgacggc gtgaatcagt ccgatgttgc cttcctgaac gagatcggac
    36361 agcttgaagc cgtaactccg gtattcatgg gcgattttca cgacaaaccg caaattggaa
    36421 acgatcagtt tttccgccgc ttccatgtcg ttgtcctttt tcagccgcat ggccagtttg
    36481 aattcttcct cggcggtcaa aaggggaaag cggttgatct ccgcgatata taagtccaga
    36541 gtgccggtga cgacgggtaa cttcaacggg cgggcctcct cagtccaaaa aacctgatct
    36601 ggtagatacc gaagagtgaa tgagcggtca agcaaatatt tcccggcggc cgtccgcggc
    36661 ttgacaggct ggcttcaaat gcttatttta ggctcaaaaa agaaccggga atgaatatga
    36721 aatccgtgaa caagacgatg atcgaaacgg ccatcaagct cgcgaaaggc gtcaaggccg
    36781 gctgcgttct ggtctgcgtg gatgtccgaa gcgaactggc cgagctttcc gaggaagaga
    36841 gaaaatcagt ccggttcgtt ctcgttctgc gggaggacga ggagctgccg gagaagctgc
    36901 tgccggcggc aaagaaactg gagcttcccg acgtcaatct gacccgcgtc gggaaaatca
    36961 agatcgccat tgccaagggc atcgtctccg gcctttttaa aaagggcgac aaggtcgtct
    37021 gcctgtccgg cgtgccgaaa ttcgggtatg cggacagcat ctttttcatt gacgtcggcc
    37081 gcgagttcga aatcctgaca tccgacgaca tcagcgatgt cgtggaagcc gtgcggcccg
    37141 aggttttcaa cgccgttttg aacatcgcct gcgaactggc ggcccagggg cgcgaaaacc
    37201 ggaaggtcgg aacgattttc gttctggggg acgatgagaa agtgatgcag ctttcccggc
    37261 agatgatcat caatccgttc aagggatatt ccgaggagca gcgcaacatt ctgaatccgg
    37321 agctggagga aaccatcaag gagctttccg ccatcgacgg cgcgttcgtg atcaagacag
    37381 acggcgtgat cgttacggcg ggacgccatt tgaacgcggc gctggacagc aaagatttcc
    37441 cttcgggcct ggggagccgc catatcgcag cggccggcat caccaatgtg acccgcgcgg
    37501 tggcgatggt gatctcccag tcttcgggaa acgtcagcgt ctttaaaaac ggaaagcttt
    37561 tcgtgacgat tgaaaaaccc gtggagtaga aagaggaaga ccatgagatt tgataaattt
    37621 accttgaaag tgcaggaggc gcttcaggat gcgcagacgc tggccggaac gctcggccac
    37681 caggccatcg agccggagca tctgctcgct tcgctgctgc agcagcaaga cgggattgcc
    37741 ggcgccattc tgaataaatt aaacgtttct ccccgaaagg tggagcagga actccatatt
    37801 gatctgaaaa agcagcccgg cgtcgaaggc gcgggcgccg gtcaggctta cctgggcggc
    37861 aggctcaaca agctgttgga taaggcaatg accggcgcgc tccagctcaa ggacgaatat
    37921 gtcagcgccg agcatgtgct ggtggccatg tccgaggaaa aggaagggaa gacgggtaaa
    37981 attctgcggc aaaacggcgt cacgaaagac aatatcttaa aggtgctcgt ggacatccgc
    38041 ggcaatcagc gcgtgacgga ccccaatccg gaaggcaagt atcaggccct ggagcgctac
    38101 gccaaagact tcaatgagct ggcccgtacg ggaaagtttg acccggtcat cggccgggat
    38161 gaggaaatcc gccgcatcat gcaggtgctc tcaaggcgca ccaagaataa tccggtcttg
    38221 atcggagagc cgggcgtcgg caagacggcc attgtggaag gcctcgccca gcgcatcgtc
    38281 aacggcgacg tgccggaaaa tctgaaaagc aagcgcgtga tcggtctgga tgtcggcgct
    38341 ttgatcgcgg gcgcgaaata ccgcggtgaa tttgaagaaa ggctcaaggc ggttttgaag
    38401 gaagtcatca gcgccgaagg cgagatcatc ctttttatcg atgaaatcca tacggtggtc
    38461 ggcgcgggag cctcggaagg atcgatggac gcctccaaca tgctcaaacc cgccctggcg
    38521 cgcggcgagc tgcgctgcgt cggcgcaacg acgctcaatg aataccgcaa gtacatcgaa
    38581 aaggacgcgg cgctcgagcg gcgctttcag ccggttttag tcaaggagcc gacggtggag
    38641 gataccatcg ccattctgcg cggcctcaag gagcgctacg aagtgcatca cggcgtgcga
    38701 atcaaggatt cggcgattgt ggccgccgct accctgtctc accgctatat cgccgaccgg
    38761 ttcctgccgg acaaggcggt ggacctgatc gacgaagccg cgtcccggct gcggatcgaa
    38821 ctggattcga tgccgtccga aatcgacgcg atcgaccgcc gaatcattca gctcgaaatc
    38881 gaaaagcagt cgctcaaaaa ggatacggac aaggcggcgc tggagcggcg cgacaagatt
    38941 gaaaaggaac tggccggact ccaaagcgag atggaccgga tgaagatgca ctggtcgtcg
    39001 gaaaaggaca tcattaaaaa gattcagcag atcaaaagcc aaatggaagc gctgaaaacg
    39061 gaggaagccg gcgcgcagcg cgaaggcaac ctggcgcgtg cggccgaaat ccgctacggc
    39121 aaaatggtcg agctttccaa agcgctggaa accgatcagg caaagctttc ggacgtacag
    39181 aaaaacaacc ggatgctcaa agaggaagtg gacgccgagg acgtggccga agtcgtttcc
    39241 aactggacgg ggattccgct ttcccgcatg atggagagcg acgtgcagaa gctgatccac
    39301 atggacgagc ggcttcaaat gcgcgtgatc ggtcaggacg acggcatcgg ggctgtctcg
    39361 gcggcgcttc ggcgcgcgcg ctccggcctg caggatccga accgtccgat cgggtcgttc
    39421 atcttcctgg ggccgaccgg cgttggcaag acggaacttg cgcgggcgct ggcggagttc
    39481 atgttcgaca acgagcaggc gatgatccgc atcgacatgt cggaatacat ggaaaaacac
    39541 gcggtggcgc ggctcatcgg cgctccgccc ggctacgtgg gctatgatga aggcggctac
    39601 ctcaccgaag ccgtccggcg caagccttac agcgtcctgc tgtttgacga aatcgaaaag
    39661 gcgcatccgg atgtgttcaa tattctcctg cagattctgg atgacggaag gcttaccgac
    39721 gggcacggcc gcacggtgga tttcaaaaac acggtcgtga tcatgacctc caacgtcgga
    39781 agcgggtgga ttcaggatcc gtcgctggag gaagaagaaa agaaaaaccg ttcgatggaa
    39841 gccctgcgcg cgacctttaa accggagttt ctaaaccgca tcgacgacat catcatgttc
    39901 cacggcctga cgctcgagga cattcaccgg atcgtcggca tccagctcgg cctgatcggc
    39961 aggcggctcg cggagcgcaa gctctccatc gaattgacag aagcggcgaa aaaatatata
    40021 gccgaagagg gctacagccc ggtttacggg gcgagaccgc tcaagcgggc gctgcagaaa
    40081 cttgttctgg atccgctggc catggacctg ctggccggta aattctccga aggcgaccac
    40141 atcgtcatcg gcgtttccaa aggggagatt acgttcagca agaaaggtta aggacggaag
    40201 gccgaaggct gaaggctgaa ggctgaagaa aggctgaaga ctgaaggatt aaagggtagg
    40261 gaacggtcgc gaccgttccc tgcacaatca ggggggattt tatggcggaa gagaccaaag
    40321 gcgaaggatt tgtcataaag gacaggcgtt tttcggaaga gccggacaca cagacaaaag
    40381 aggagccggc atccgcaaag acggaggagc cgaaagaaac ggccggcaaa acttccgcgg
    40441 aaccggcgga ttatcccgcc gtgaatttca cgaactttgt catttcgctt tccacgtccg
    40501 ctctttttca tttcggcgat tttccggagt acgaaggagg caaggccgaa aaaaatcttc
    40561 cggccgccaa gcagaccatc gatattctgg atatgctcca gaagaaaacc gccggaaatc
    40621 tggacccgaa cgaagccggc ctgatcgagg gcgtgctcta cgagctgaaa atgcgctatg
    40681 tcaaagaaaa aggctgaaac gcgaaggttg gcgcccgcca aaatcaacct ttttctgcgt
    40741 attttgcgca aacggccgga cggttaccat gatctggcct ccgtgatgca gcagatctcc
    40801 ctttacgatg aaatgacctt ttccctccgc tcaaaagaaa ttgttatttc ctgccccggc
    40861 gctgatcttc ccacggatga aaaaaacctg gtttacctcg cggcccgaaa gcttttcgac
    40921 catgccggct attcgggcgg cgtcgccatc gaacttgcca agcagattcc cctggccgcc
    40981 ggtctgggcg ggggcagttc ggatgccgcg gccacgctgc tggcgctcaa tgacctgtgt
    41041 tccctggggc tctccaaatc cgaactgatg aaactggggg caaaactggg cgcggacgtc
    41101 ccttttttca tgttcggccc gacggcgttt gcctccggca tcggcgacag gctccagacg
    41161 cttgaaaata caatcaaatt cagcatggtg ctgatcaatc ccccttttcc cctttccaca
    41221 aaatcggttt acgaaaagct taatttcacg ttgacaaaaa aagaaaataa ttatagcatt
    41281 ccgcgatttt acgcactggg tgatgtaatt cgggaattgc ataatgattt agaagctgta
    41341 tcgatgaaca tgcatcccga attggcggat tttaaacagt tgctgatcaa cgaaggcgcc
    41401 ctgggtgcat tgatgtcggg cagcggtccc acgctatttg gcctttttgc cgatgaagag
    41461 tcggccagag aagcgcggag tgctattgaa aaaaaagttt ccggagactg tcgcgttttc
    41521 tttgcccaat cactctcggg taaatacgaa ttctcccctg acgaagaact gcccggttga
    41581 attataaatc gaagacgttg gggcgtcgtc aagcggtaag acacaggatt ttggttcctg
    41641 cattcggagg ttcgaatcct cccgccccag ccagaaaagc tttgggagaa atatttaaat
    41701 gcttgaaaga ataagggtat ttaccggaaa tgccaatcgg gaactggccc ggaaaatctg
    41761 tgaaaagctg ggggttccgc tgggcaacgc caatgtaacc accttcagcg acggtgaaac
    41821 ccgcgtggag attgatgaaa atgttcgggg gatggatgtt ttcatcatcc agtccacctg
    41881 cacgccggtg aatgtcaccc tgatggaact gctcattatg atcgatgcca tgaaaagggc
    41941 gtctgccgac cgcatcaccg ccgtcgtgcc gtactacgga tatgcgaggc aggaccggaa
    42001 agtggcgccc cgcgcgccca tttcggccaa actggtcgcg gatttgatta cgacggccgg
    42061 cgccaaccgt cttttgtcca tggacctgca tgccggccag attcagggct tcttcaacat
    42121 tcccgtcgac aacctctttg ccacgccggt tctgatagac tacatgaaga aaaaatacac
    42181 ggatgatacg gtagtggttt ctccggatac cggaggcgtc gagcgggccc gggcgttcgg
    42241 caaaaggctg ggcgcctctc tggcgatcat cgacaaacga agggaaggcc ccaatgaagc
    42301 ccaggtgatg aacatcatcg gcaacgtcga ggggaaaagg gtcattatcc tggacgatat
    42361 ggtcgatacg gcggggaccg tcgtgcaggc ggccaaggcg ctggcggaag cgggcgctct
    42421 ggaagtgtct gtctgctgca cgcacccggt tttgtccggt ccggccatcg atcgcatcga
    42481 gaaatccgcg atcagggagt ttattgtgac ggatacgatc cctctttgcg aaagggccgc
    42541 cggctgcgaa agaatcaagg ttttgtcggt gtccggtctg ttgtccgaag cggtgcgccg
    42601 tatttattac aacgattcag taagttcatt atttatttag gagggaaggg gtaaatggaa
    42661 ataaccgatt tagcggccca ggtccgtaaa gaacagaaga agggccctgc ccgaaggctg
    42721 cggcagcagg gacttgtgcc cgccattttt tacggcaaag ccacggaaaa catccagttg
    42781 gccgtgaaaa gcgccgacct gtttcggctg cataaggaaa agaaagacca cgctttcatc
    42841 aagctgatca tcgacgacgg cggcagcaaa gtcgaaaagc tttccctcat caaggaactg
    42901 cagacccagc ccctgagcgg aaaattctac cacgcggatt tttatgaagt ggacatgaaa
    42961 aagaaactgg cctttgacgt ggagctgaac tttaccggca gggcgatcgg cgtggaaaac
    43021 ggcggggaac tccagcacat caaaagagac atcagggtgc tgtgcctgcc gacggatctg
    43081 cccgaccaca tcgatgttga tatttcggcc atcgacatcg gacattcgct gaaagtgaaa
    43141 gacatcgtca ttccagaggg tttggcccat ctggatcctc cggaagccgc cgtcgtggca
    43201 gtcgccgcca ttcgggtggt caaggctgag gaagcgcagg aagcgccggc ggaaggcgct
    43261 gaacccgcgg aaggcgcagc agccgctgaa ccggcaaaag aggacaagaa ggaaaagaag
    43321 taaggcgaca ggtgcattat acgggagatc atgcgtctct tcagctttgg aaaatatctg
    43381 ttgggttttc ataaagagag gggacaaatg gtttatctgg ttatcggact gggcaatccg
    43441 ggaaagcgct atgaatccac ccggcacaat atcggcttca tggtggtcga gaagctggcc
    43501 gaaaggtgga aaatagagct gaaacataag agtttcaatg ccctgtgggg caaaggggcc
    43561 atggacaaaa aacaggtcct gctggccaag ccccagacgt tcatgaattt gagtggtact
    43621 gccgcaaggc ggctgcagtc gttctttaaa acggatatca gcaatctcat cgttattcac
    43681 gatgatctcg acctgccttt tggctccata cgcatgaagg cgggcggggg aactgccggt
    43741 cataaaggac tgtcatctat cgagtctaac ttgggaaatt ccggttttat tcgggtgagg
    43801 ctgggtattg gcaaaccagt tgacaaatcc cgaatagagg gttatg
//
LOCUS       DAOPAF010000008        43417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1010_length_43417_cov_43.564457, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000008 DAOPAF010000000
VERSION     DAOPAF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 43417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43417
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_43417_cov_43.564457"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLQ77_01765"
     CDS             <1..327
                     /locus_tag="PLQ77_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09437.1"
                     /translation="GRVKLDVRLDNETVWLTQQMMADLFQTSKQNISHHIQSIYDEGE
                     LLTEATVKKYLTVRVAHRAGAWNEARIILLACRSDTCCGPMACMPTVQVRSSIAKETH
                     ELGNTQ"
     gene            503..1270
                     /locus_tag="PLQ77_01770"
     CDS             503..1270
                     /locus_tag="PLQ77_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPL09438.1"
                     /translation="MNITKNIKKITMLLTVLMLVALATGCVSWSNTPLHVLRYDQPGQ
                     HEERRLIVFLRGMGGGNESFEEQGFVDEVLARGLAYDMAAPDVHIGYYMDRSMIQRLK
                     EDVIDPAKAEGCRKIWLVGASMGGLGAILYLKEYPEDVAGVYLMAPFLGTQSILDEID
                     AAGGLSCWKPGALQDDDDWQRMLWTWLKTEVAGRPEKIVYLGYGTEDFYGKGALLLAP
                     VLPPDRVYAIAGGHDYSTFKALWKMFLSGSAPLRDEP"
     gene            complement(1411..2121)
                     /locus_tag="PLQ77_01775"
     CDS             complement(1411..2121)
                     /locus_tag="PLQ77_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPL09439.1"
                     /translation="MRNSLAIISLAPVLIAQALYVRRVTPRLPEPLGERSGEHGSGPP
                     LKLLIAGDSAAAGVGVSRQSEALSGQLVRALGQNFRVSWKLIARTGHRSKDVLAMLEE
                     IPPEKLDVVVTSIGVNDVTHGTGRQTWIARQEKIIRLLLSKFDSPHVILSGIPPMHLF
                     PALPQPLRWYLGSRAKHFNHALEQSVQGCSRCTFVSIDFPLETTYMADDGFHPGTRAY
                     SFWAKHLAGIITAKIESR"
     gene            complement(2166..2645)
                     /locus_tag="PLQ77_01780"
     CDS             complement(2166..2645)
                     /locus_tag="PLQ77_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPL09440.1"
                     /translation="MEKTITKVYVDNEGFVVFVCSKCNLIQKQEAKTYGGAKGPIQVQ
                     CTCGNVYDVEIEFRKFYRKGIRVDGLYFGVTNPKIWGKMVLKNVSMQGCGFETMGVNP
                     LQPGDLIKIEFQLNDAKRSVIKKQAAVRSIFKKYVGCQFQEPPNSMDADLGFYLRTP"
     gene            complement(2765..2980)
                     /locus_tag="PLQ77_01785"
     CDS             complement(2765..2980)
                     /locus_tag="PLQ77_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPL09441.1"
                     /translation="MSMDITAPMPGKIASIAVNVGTQVQEEEEVIIMDAMKMEIPVYA
                     PGAGTIKEIKVKVGDSVNEGQVLAVLE"
     gene            complement(3136..4365)
                     /locus_tag="PLQ77_01790"
     CDS             complement(3136..4365)
                     /locus_tag="PLQ77_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09442.1"
                     /translation="MINFLSTAKISLRALRANKMRSALTMLGIIIGVAAVITMLAVGR
                     GASNKISEQIASVGSNLIIVLPGSTTASGIRLGSGATQNLTRSDAEAIKQECSAVATT
                     APILNGSAQIIYGNSNWPSIVFGTDENMLEVREWTLASGRNFYDQEIRSAAKICLLGQ
                     TVVDNLFGNDDPLHKTVRIKKVPFVVIGVLAPKGQSMLGQDQDDIVYVPVSSAQRNLF
                     GRWFPGTVRSIMVKARSTEDLDRAEQQIKELLRQRHRIGPDQEDDFTVRNLTQMMQMA
                     EQASRVMALLLAAIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEAMILSLIGGLIGVLLGILFAELLSFFAGWTILISTYSILLSFGFSGLVGIFFGFY
                     PAYKASLMNPIDALRHE"
     gene            complement(4362..5051)
                     /locus_tag="PLQ77_01795"
     CDS             complement(4362..5051)
                     /locus_tag="PLQ77_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09443.1"
                     /translation="MALIETDHLEKRYEVGDSIVHALDRVSLQIHQGEFVAVMGPSGS
                     GKSTFMNVIGCLDVPTAGTYRLDGIDVGSMSRDALAEIRNEKIGFVFQGFNLLARTTA
                     LENVEVPLLYNKDGSKNRRERALDALRTLGLEDRRHHHPNQLSGGQQQRVAIARALVN
                     NAPLVLADEPTGNLDTKTSIEIMELFVRLNEEKNITIVLVTHEPDISAYSKRIIHFLD
                     GKLVSDSVNKR"
     gene            complement(5057..6223)
                     /locus_tag="PLQ77_01800"
     CDS             complement(5057..6223)
                     /locus_tag="PLQ77_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL09444.1"
                     /translation="MKKIIAASVILILILIALFFFLSRREPAPQYITQTADRGDIRAT
                     VSATGTVNAVTTVLVGTQVSGTIKELYVDYNSTVRQGQLLARIDPASIEAQVAQAAAN
                     LSLARANLEKSKVALRDTVSVFERNKILYGKNFISRHDLDTSETAYLSALAQIKASEA
                     QVEQTRAALNLAETNLRYTRIVSPVKGTVISRSVDVGQTVAASFQTPTLFTIAQDLTK
                     MQIVANIDEADIGRLRVGQPVTFTVDAYPDLTFGGNVSEIRNSPTTVQNVVTYDVIVK
                     VDNSDLKLKPGMTANVSIVIDDKKGILRVPNAALRLKIQDKSSSAPAPKGPGVWVLEN
                     KKPKRVPLSIGISDNRFTEVVSGKILQGAPVIVEVKSNDQKNAATQTPRPGPRF"
     gene            complement(6220..7500)
                     /locus_tag="PLQ77_01805"
     CDS             complement(6220..7500)
                     /locus_tag="PLQ77_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPL09445.1"
                     /translation="MRKRIFLLFLLLFIPAQSLFAAGDIQKGDVLTLRQCIDIALKNH
                     PALNAATGAIHQSESKIGKARAGYYPQLSFQSGYSRIGPPASSLTNSSYDYYSNALNL
                     NQTLFDFGKTSTAVDIQKLGKQSAEADFQDTTSLLTYNVQEAYFSLLKAKMSETVAVE
                     TVNQFQQHFEVAQTFFETGKSSKIDVTSAEVNLSNARIQLISAQNALRLWRVNLNKAM
                     GVINAPEYDIQEKFNMDAADITFEAALARAYENRPDIQSIALKKDALERTVDLNKKDY
                     LPVLSGSAGYGYAGQDAALNQSWNVGVALTFPFFTGFSTKYAVEEARAGLEIAKANEE
                     ALRQKAFQEVKSAWLNRHEAFERIEAGKITVRQAEESLELAQGRYATGVGNSLEIVDA
                     MIKLNNAKMTYISALSDFNIAEASLEKAMGAKMP"
     gene            8006..8878
                     /locus_tag="PLQ77_01810"
     CDS             8006..8878
                     /locus_tag="PLQ77_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPL09446.1"
                     /translation="MRIFVRKWSALVVLLCLVLVAGCASLSSQKSDQFIFLSSTIGPI
                     DSGIVGALEDQFEKETGIRVRHVGAGTGAALKLATKGQIDLVMAHAKSLEENFIADGY
                     GTERIPLMYNDFVIVGPASDPAAVKGEKSATAALKKIMDANGKFISRGDKSGTHVAEM
                     ELWKKTGVEPKGAWYVIYEKGSEGNVATLKYTDQQAAYTVIDRATFLSLKDQIKVVIL
                     VEGDEALLNYISLIPVNPAKFPRVNRKDTLTFVQWLTSPEKGQLIIRDFGKEKYGSPL
                     FFPNSVEWQKLQKK"
     gene            8903..9769
                     /locus_tag="PLQ77_01815"
     CDS             8903..9769
                     /locus_tag="PLQ77_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPL09447.1"
                     /translation="MKMFKSSKWFIILAVVAALVMLGAAGAFAAPKQKNIILATTTST
                     QDSGLLDTLIPIFEKETGYFVKTIAVGSGQAMKMGEKGEADVMLVHSPDAEKKFVEAG
                     YGINRRLVMHNDFIIVGPPNDPAGIKGVKSSSDALKKIAGANALFISRGDNSGTHAKE
                     KTLWKKANVNPAGQKWFQETGLGMGQTLNVASEKKAYTLADRGTFLAIRKNLGLTILN
                     EGDAALLNIYHVIEVNGAKWPKANTEGAKAFADFMVSKKTQSIIKTFGVDKYGSPLFF
                     PDAGKKVEDLGK"
     gene            9778..10476
                     /locus_tag="PLQ77_01820"
     CDS             9778..10476
                     /locus_tag="PLQ77_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09448.1"
                     /translation="MDLIVQGVIKAFQLIISFDPEVMGITWLSLKISGTATFISLFFG
                     VSVGTAVALNNFPGKRLVISIINTGMGLPPVVVGLFVTIMIWRSGPLGFLEILYTPYA
                     MIIAQAIIATPIVMGISLASIQNLPPNLRLQILSLGASRMQMIWVLIKESRLPLLAAV
                     MAGFGGVISEVGASIMVGGNIKGYSRVLTTATVMETGKGNFDIAIALGIILLILAFLV
                     NLLLTQIQQRQHPR"
     gene            10473..11558
                     /locus_tag="PLQ77_01825"
     CDS             10473..11558
                     /locus_tag="PLQ77_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09449.1"
                     /translation="MNNIFTIENLIVRRGGVKVLDINELKVHESRILALIGPNGAGKS
                     TLLLTMAGLLKPDQGKMHYKGRPVESSAERSRMRRSVCVVFQEPLLLSTSVYKNVALG
                     LKFRGMSKNDIQKNVGDALSYFGISSLANRSAKTLSGGESKRVSLARAFALKPHLILL
                     DEAFNSLDPPTRESLIDDLRHILEETKITAVLALHDREETLRLAQDVTVMSEGRLIQT
                     GATAQIFNQPQSEFVANFVGTETILEGTVQNIRSGLMEVLVSGKVIEAAGAAPLGQKV
                     FCCIRPENIVVSREAPENTSARNIYEARIQKIVRQAFSYKLVLDCGFPVVAYLTVSSC
                     EDLGFCEGAAVFASFKATAVHVMRREK"
     gene            complement(11645..12643)
                     /locus_tag="PLQ77_01830"
     CDS             complement(11645..12643)
                     /locus_tag="PLQ77_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HPL09450.1"
                     /translation="MALFEGYERRIKQITPFLAKYGISSLEEAEKVCIGKGVDVRKIV
                     KDIQPISFENAGWAYLVGAAAAIKKGQKNAADIAETLGEGLQSFCIPGSVADDRQVGI
                     GHGNLASMLLREETACFAFCAGHESFAAAEGAIGLAKSANKARKNPLRVILNGLGKDA
                     AHIIARVNGFTHVATAFDYKTGKVSVAREKAYSKGDRAAVRCYGADDVREGVAIMHLE
                     GVDVAITGNSTNPTRFQHPVMGVYKKERLLAGKKFFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAVEQAMK"
     gene            complement(12655..13347)
                     /locus_tag="PLQ77_01835"
     CDS             complement(12655..13347)
                     /locus_tag="PLQ77_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09451.1"
                     /translation="MEHTHEIEQMCVVAKGAKNGPAPIPQEGGWTPVKEIKDISGLTH
                     GVGRCAPQQGACKLTLNVKKGIIEEALVECIGCTGMTHSAAMAAEILPNKTILEALNS
                     DLVCDAINVAMREIFLQIVYGRTQTAFSEGGLPIGAGLEDLGKGLRSVIATMYGTKVK
                     GPRYLEMAEGYVTEIGLDADDEIIGYKFVQLGVMMDNITKGMDPAAALSKASGTYGRF
                     AEAVKVINPRKG"
     gene            13674..14099
                     /locus_tag="PLQ77_01840"
     CDS             13674..14099
                     /locus_tag="PLQ77_01840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPL09452.1"
                     /translation="MNYQLYLEKIRAGEKGLNPYLDHFNVEVAGLSSGRAVFRMPIRP
                     EYMQGAGLMQGGLMVALADEAIAHAMMTVLTPEEGLTTIELKSNFLAGVDKGELIAEA
                     GVFKKGRSLIIGDCQVTNGAGKPVCRVSATFMLLRQHQK"
     gene            complement(14157..15491)
                     /locus_tag="PLQ77_01845"
     CDS             complement(14157..15491)
                     /locus_tag="PLQ77_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPL09453.1"
                     /translation="MNPHVFREYDVRGIVDKDLNEVFIVNLGRAIGTYALQHKVRTMT
                     IGRDCRLSSDDYHRFLIAGLNASGMDTIDIGLCATPMLYFSIRRLGAGGGVMITGSHN
                     PPEFNGFKICIGYDSIHGEQIQELRRIMESGSYHSGKGSAQAQDITKIYQDYIFDSVR
                     VSKKLKIVLDGGNGVGGKFALPILERMGCDVTALYCEPDGRFPHHFPDPTVEENLAEL
                     IRLTAKEKADLGIAFDGDADRIGVISDTGEIIWGDKLLLLFARFILKDHPGATIIGEV
                     KCSQVLYDGIAEYSGRPLMWKAGHSLIKAKMKEEKALLGGEMSGHLFFADRYFGYDDA
                     IYAAVRLLEILSQTGLKLSELLAGVPKTFATPEIRMDCDDDRKKAVVAKIKEHYQNTP
                     GVIDIDGVRVPFGDGWALVRCSNTQPVIVLRFEASSAARLQEIRGEVEGLLA"
     gene            complement(15588..16274)
                     /locus_tag="PLQ77_01850"
     CDS             complement(15588..16274)
                     /locus_tag="PLQ77_01850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPL09454.1"
                     /translation="MAPIPFDRSSIDTVIFDFDGTLARLNIDFEQMRRDVASLIDRWG
                     VDPRSLQNRFVLEMIGEVQALLGKNSGDHAASFTREAFRIIEEIEVEAAQSGSLFDGA
                     KELFTGLRKASLQAGIITRNCEKAVRTVFPDIHDYCPVVVCRNDVRRVKPHPEQISLA
                     LSRLGAKPGRCVMIGDHPIDIETGKNAGTRTAGVLTGRFQEKDFLRAGADMVLREAPD
                     ILRRLKPEDF"
     gene            complement(16279..17118)
                     /locus_tag="PLQ77_01855"
     CDS             complement(16279..17118)
                     /locus_tag="PLQ77_01855"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPL09455.1"
                     /translation="MTMDLTLLRDKDAFPLYVLERLNRTPVDYLEKHLSIERSNAAGG
                     KHLEAGVVLLLLFKNSEFHFLLIKRSNAVAQSGDISCPGGMLERPVDEMLSHIVSKTG
                     ILRTAGNRALHELPNKDGETQSLIRLFLMNALRESWEEIGLSPLNVEFLGALPTYSLT
                     LFTRTIFPLAGLVREPWPYRPNDEVDKMLEIPVSDFFRSSSYALLELSPENGTSDPRR
                     NFQFPCLVIPDGKGGEDILWGATFFIITNFLREVTGGAFPAETPGRIVTKTLSAHYTS
                     GNR"
     gene            complement(17115..18107)
                     /locus_tag="PLQ77_01860"
     CDS             complement(17115..18107)
                     /locus_tag="PLQ77_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPL09456.1"
                     /translation="MTAGNGNPSATFQPRSFLKNRHLQSVLASSHLMIPPHGPLRRHS
                     REFIIETPGGSKLLSYLSHHPRSRGMMILLHGWEGSSSSAYILAAGAYFFDRNFSVCR
                     LNLRDHGDSHHLNQGLFHGALIEETFDAVNAVARMAGNLPVYLLGFSLGANFSLRIAM
                     RHSRTPVGNLQKVFAVSPPLDPYRTTQTIDRGPALYRNYFLRKWKRSLRKKQRFFPDQ
                     YDFAGMLRARSCMELTEKMMAYFPEFPSYRDYFRLYTLTDNSFCDLGVPVKIFFAADD
                     PVIALDDFRKLRNHGFLTVSEQPFGGHCGFVDLFPRRRWYNEQIFEEFQQERNA"
     gene            complement(18171..18247)
                     /locus_tag="PLQ77_01865"
     tRNA            complement(18171..18247)
                     /locus_tag="PLQ77_01865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18211..18213),aa:Met,seq:cat)
     gene            complement(18315..20051)
                     /locus_tag="PLQ77_01870"
     CDS             complement(18315..20051)
                     /locus_tag="PLQ77_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPL09457.1"
                     /translation="MKKIMIGFCLLAALLMAATLTAKEKYTVTILPFALHSAENIEYV
                     RQGINDMLISRISGADKIDVTRKEVVSDVLKKTGGKELGLADVQDIGRQLNSDYVVWG
                     SITKIGKSISIDGKLIDITGGKSDVGIFSQSQTLDEVIPKINDFSQRIVRHITGGAAA
                     ESAPAAVSTAPAAAPPPVPGASREAQIIAGMKSGKGKGTLTSMINAEFINSSDPLDRK
                     KGFWMSQQFKTEFNGMDVGDVNRDGRNEVVIIDPHNVYIYLKTKDNLQLLKQIKGKSY
                     HKYISVDVADINKDGTPEIIVTSLNDRLLNSFVLQYEDGDYKTIASDIRYFLRVIIPS
                     SGAPVLLGQAYGLGSFHDGQGGRVFDTQIYEIVWRDGKYVEGDKQKIPVGLSIYGLTL
                     DTLGAGTSEKIIALDELDYLCIITPTTKPLSRIFSFGFSPDELIWRSDSQYGGSNNYI
                     DNVDKQKQQSSEATQEDSVFANLRIVMFDTNNDGKKEMIIVKNLSSVGRIFKNLKLFS
                     SSEIYNLEWDGLGMAENWRTKKINGYVADYCFKDVDNDGKPEIVLALVQSVGATLSER
                     SVLVVYELEMPQ"
     gene            20276..21565
                     /locus_tag="PLQ77_01875"
     CDS             20276..21565
                     /locus_tag="PLQ77_01875"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPL09458.1"
                     /translation="MEKATLKLNGMTCELPIVEGTENEKGIDISRLRQDTGLITLDVG
                     YQNTGSCISSITYIDGDKGILRYRGIPIEELAEKASFTEVAYLLIYGELPNREQQQLF
                     SQRLTQNSMIHEEMLRFFDGFPPTAHPMAILATMVNSMSVYYTDYFTEDFRAETFDLM
                     AASLISRIRTIAAYSYKHSIGHPHVYPKRDLKYASNFLYMMFDSPVNPYVPDPDIERA
                     LSTLMILHADHEQNCSTSAVRLVGSSMANLYASICSGICALWGPLHGGANQKVIEMLE
                     EIKGSKLSIQSCIDSAKDKTSKFRLFGFGHRVYKNYDPRARILKQYCETIFKKHNYQD
                     PLINIAMELEDAALKDDFFIQRKLYPNVDFYSGILYRMIGIPTNMFTVMFAIGRLPGM
                     IAQWKEMHDTVPLKIGRPRQIYTGSNLRHYIPMDERG"
     gene            21838..23046
                     /locus_tag="PLQ77_01880"
     CDS             21838..23046
                     /locus_tag="PLQ77_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL09459.1"
                     /translation="MKTIGRILLVMGILAMTALTGQAVAQTKAIKVGAAINLTGPASS
                     WGQFHEKGQRDYFRYVNEEKGGVGGRKIEMITMDTAYKVPEAQAAVQKFILQDKVDMI
                     ATWGAGEGLAAKPIVQKHKVPTINYSTSWEILEKPVGYMYLPFGSYKMDCRAVLEYIK
                     TIHTGNGAPKVGLLTYNNAYGRSIHAPSREYAAKLGVQLVAIEEFPPRTVDLNTELLR
                     LQKKGAQYIFIQMLPSTIITAFKSADRIKYNPLFLGTWTSTDPDFFRMGRGLIRDRLV
                     MQFPGGLPSDKAKGMEVMKELWKRYKTVKSFDASYWEGVVVGMIMERAFLRAWEKSKV
                     INPETVNAAMESMKNEDFGGLVPAVSYSKTNHEGSFTARMVKVMENGTYIPLTNFYIP
                     GKDKIKVLKR"
     gene            23128..23958
                     /locus_tag="PLQ77_01885"
     CDS             23128..23958
                     /locus_tag="PLQ77_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09460.1"
                     /translation="MKAFRSRPSIMINRPQEDKKAELEIRNLRLSFGGVVAVKDVDLS
                     VHTGELTAVIGPNGAGKTSLLNCITGFYRPQQGSIVFNGRDITHLHTHQLVGIGIGRT
                     FQNIELFPGMTVLANLTLARHIHCRYSFLHSCLFTPSVREEEIRHRRILEEIIDFLEM
                     QSIRKQTVGSLPYGMMKRVELGRALALEPRLLILDEPFAGMNLEEKEDMVRFLLELNG
                     RWGLTMVLVEHDMSIVMSISRHVMVLNFGEKLGEGAPADISANPEVIKAYLGDAQSLE
                     "
     gene            23974..25902
                     /locus_tag="PLQ77_01890"
     CDS             23974..25902
                     /locus_tag="PLQ77_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL09461.1"
                     /translation="MDKATPHPPKPNQITLPQLLAANAGKYGDTRTAIREKAFGIWKK
                     YTWGEYFSFVKRTAAGFASLNLARGDNLCMIVNNSPEWLFSELAAHALGATSLNLFTS
                     SIAEELAFSIGRVRSPLVVVQDQEQVDKLLEVKDKLTQVRFVVYVDPTGMTSYEDNPW
                     LISFAALLERGERHMADQPDYVDAEIGKGRPRDIAVMMQTSGTTGVPKIAMLSHHNLA
                     AMAHQWVEAAHIRPEENWISMSPTAWIVDQMWGMGVALAGAMTMNFPETPETVLEDFR
                     DIGPSLLITASRFWEDMASRIRVKITDAGWLKRKLFYLGESVGREVVGKKLRRESISW
                     PLGLLYRFLTLAVYNPLLDRLGCSRFHSAFTGGHPISPDVIGFFRAIGLNLKQCYGLT
                     ESCGIFQIQPDDEVKLETVGKPLPNTSIKLAEDQEVLVKSEANFSGYYNDYQSTEAAF
                     ENGWLKTGDAGYIDEDGHLLIIGRKEDIIRNKSGDAFSPDFIETRLKFSPYIKEAVTF
                     GEARPFITAMINIDLGNVGSWAEERMIPYTTYMDLSQQKAVEEMILREVREVNGQLPE
                     VMKIKKFVLLYKLLDADDDELTRTGKVRRRFVYGLYIKIIEAMYAGAQNVPVTGKIRY
                     RDGRIGEIETIINIISVD"
     gene            25914..26792
                     /locus_tag="PLQ77_01895"
     CDS             25914..26792
                     /locus_tag="PLQ77_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL09462.1"
                     /translation="MEFFLQLLVNGVCIGLLYGISAMGFVMIFKASSVLNFAHGELLA
                     LGAFFFLSLVTWGKIPVAAAFLLTLIGCFVLGVLIEKFFLRPLIGEKLIFVIMLTVGL
                     AAMFKGLILLVWGGNLHTYPEFLPASLEFHLGSIYIAPVYSATLVISVVFLVLFGLFF
                     KKSSQGIFMRSVADNQKAALSLGVHVRRVFALSWAIAAMVACMSGIVLGIINGVNVHD
                     LSAIGLKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIVLVFIL
                     MVKPYGLFGLVEIERV"
     gene            26794..27864
                     /locus_tag="PLQ77_01900"
     CDS             26794..27864
                     /locus_tag="PLQ77_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL09463.1"
                     /translation="MKKPSFHPCGNFRETYAEELAIFDTWFGRVSLIVFLLLLFCLAP
                     LMLDRYLLYILNSIGIMAIAAIGLNILIGYTGQISLGHGAFFGVGAYAGAILATRLNL
                     PFFVCIPAAGLITAMVGMIFGIPSGRLKGLYLTIATLAGQFIIEYVLIQWESLTKGTM
                     GITLPEPQVLGVNISGDKAFFFLIFACLVCMTWFAVNIMRSKYGRAFVAIRDNDRAAE
                     GMGIPIFRYKLLSFAISSFYAGFAGSLWAYYMVSITTEPFNLALSVEFIAMVIIGGMG
                     NIPGAIFGATFITVLNELLRFATGALMNVGWIAGLGLNMAPLREFVFGLAIVLFILLE
                     PKGLAELWRIVRSSFRLWPFSY"
     gene            27950..28750
                     /locus_tag="PLQ77_01905"
     CDS             27950..28750
                     /locus_tag="PLQ77_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09464.1"
                     /translation="MGEILLQLNNISVVYSDVIQVLKGVSLSVANGHIVSLLGSNGAG
                     KTTTLKAISGLLKPENGEVTDGSIIFNGVNIQNQAPEFITRQGIIQVLEGRQPFKYLT
                     IEENLRVGTATRPGKPYKEDLEMVYSYFPALVKRKKALAGYCSGGELQMLVIGRALMA
                     HPQLLMLDEPSLGLAPLVVQEIFRIIRKINEEHGTTIVLVEQNAHMALQVAHYGYVME
                     NGKIVMEGTARELADNPDVKEFYLGMAASGIAKSYKDVKSYRRRKRWL"
     gene            28765..30018
                     /locus_tag="PLQ77_01910"
     CDS             28765..30018
                     /locus_tag="PLQ77_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL09465.1"
                     /translation="MKRLYDEKEAWSPEKRKAWQDRRLSRMVRLGYQKSPALRRMLDA
                     NKINPARIKTREDLEALPVTSREKLVEMEMRKPPYAGLENSTIAVDRIFTSPGPVYEP
                     HLSENDFLWARAFFAAGIGSRDIALNAFSYHLVAAGLTFHNGLRKVGATVVPSGTSSS
                     QIQVQLIRDLKVTAYVGTPSFLMSIIDQSKEMGYDFKKDFRVKKACFAAEPLLPSLRQ
                     RFEKEYGIRTFQMYGATEVGDVAYECPAKSGWHICEETIVEIVDPATGKNVAPGELGE
                     VVVTRLNDLFFLLRFGTGDLSRLVTKKCSCGRTSFRLAGIAGRVGDAVKVRGLFIAPS
                     QMKLLSAKFEGLPLQAVVSRKGHEDVLTLRIEKINSQVNTDSWKNNFKKIFKELCSVR
                     IDVLEVVEKGGIKTDQKVIVDSRTW"
     gene            complement(29975..30352)
                     /locus_tag="PLQ77_01915"
     CDS             complement(29975..30352)
                     /locus_tag="PLQ77_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09466.1"
                     /translation="MNDATRAALFSFLLIPGGGQIYLKRYARGMAFLIPAAAATLTLA
                     WMIFQVVLMIVRDAASEGAGTTRILRNAENALRTIDLNLALGLLILILFLWISSTIDA
                     YRLGKKTEPPTTSGNRRSPSDPF"
     gene            complement(30362..32101)
                     /gene="recJ"
                     /locus_tag="PLQ77_01920"
     CDS             complement(30362..32101)
                     /gene="recJ"
                     /locus_tag="PLQ77_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPL09467.1"
                     /translation="MNERGQLPVTQWKIKDSGPKTIEESLVREFGFHPIISQMILKRR
                     VGSLEDAHRYLHPSLNDLHSPFLMQDMKKGVARLLKAIYDGEEIVIYGDYDADGITSV
                     VILYKFMKQLTARVSYYIPDRVQEGYGLKIPVIDRFKQKNVKLIVTVDCGISDIEQIA
                     YAKSVGIDTIVLDHHEIADQLPPAVACINPNRSDCSFPFKHLAGVGIAFNFLIALRGT
                     LRREGFWKNDDYPNLKEFLDVVALGTIGDIAPLLDENRIFARIGLDLITEGRRPGIKA
                     LKEVSGIDGQLVDSFKASFCLIPRINAAGRIASPLDAVELLLAETMEEARPLAEKLDL
                     HNRRRQAMEKDILNDILEKLGAKPDLENISALVFSSEKWHPGVVGIVASRLVDLFSRP
                     TFVISLKDGVGKGSGRSVSDFNIHKGLQQCAPLLLSYGGHYRAAGISIKEDDIDEFAC
                     LLDEIIRNTVQSSEMVSQTLIDAECRLQDISLDLIRQMSMLAPFGCENPEPVLCSRNI
                     KASSPVIVGNNHLKMRVVDDGASHSAIWFGMGKYLGVINQASFDVVFSPQINYWNGSS
                     DVQLKIRDAAIVA"
     gene            32253..33041
                     /locus_tag="PLQ77_01925"
     CDS             32253..33041
                     /locus_tag="PLQ77_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09468.1"
                     /translation="MLMLTHTCLLQQMMSASGIQNMDPDIYIYNIAPDLLTIHPDINP
                     RQTHSIPRFIEAPPDHLRTAYIMFHLLVDDLAHYGSISMTCKEGFDPDSSGYTYLKGR
                     NLVRSIMELHAIIGKDISYSEAAYRSHLIIEMIYDLIIGPQIDKNGSIDLLEEAIHFT
                     FDRREKEFCSDISWLYGIDFEQSREVLKIAVSYITGERLRRIMNIEGRIRLFTDKFGL
                     RNNNEMFAEAIAALFQDALDSIENDDFLQETASTIRSCGWLPTR"
     gene            complement(33038..33235)
                     /gene="rpmB"
                     /locus_tag="PLQ77_01930"
     CDS             complement(33038..33235)
                     /gene="rpmB"
                     /locus_tag="PLQ77_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPL09469.1"
                     /translation="MSRICEVCGKKPAIGNNVSHANNKTRVIWRPNLQKLRCVDEKTG
                     AVKRVKVCTRCIRSGFVKKAS"
     gene            complement(33399..34697)
                     /locus_tag="PLQ77_01935"
     CDS             complement(33399..34697)
                     /locus_tag="PLQ77_01935"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPL09470.1"
                     /translation="MQDDFAISVLGVCAIDSPLAINGFVSDDDRILFNTRLKDCDALM
                     VSTGKPLSLEPAGPRRKIFFDPAGTKAAIVTCGGLCPGINDVIRSVTMTLFYRYGVKN
                     VLGIKYGLRGLNPEYGKIPIQLTPDFVKDITHIGGSILSSSRGPQDVKVMVDYLELLK
                     IDILFCVGGDGTMRAAEKITEEIHARKDPIAVIGIPKTIDNDLNLIEKSFGFDTAIAK
                     TVEAVGSAHVEAKGAYNGIGLVKIMGRLSGYITATTALAQGDANFVLIPEVPFDLEGE
                     KGFLKALENRMKASGHAVILVAEGAGQELMQQDEKKRETDASGNVRLQDIGLFLKSAI
                     ETHFREAHVEINLKYIEPSYMIRSVPANASDSLYCSSLGQYSVHAAMAGKTGLLVALR
                     RDEYVHLPLSAAISGRQIDPDGNLWFRVLESTGQPAQMKN"
     gene            complement(34702..36144)
                     /locus_tag="PLQ77_01940"
     CDS             complement(34702..36144)
                     /locus_tag="PLQ77_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09471.1"
                     /translation="MYDLLVLGDDLASHVAAACASRNGLKTLLVAPGGLGGLQLIGDF
                     VFHLDPAPMTGLAPGQPGFSVLAELGVEIPETHPTSMSPAYQVLLPGHRIDFSNDSEA
                     LLSELAREFPKRETDIRDFYGIALQASSVFQDWIAEHPRVQPQTLKEYFSYLKIYPFV
                     LQYKFAAARFDKMLSRDAALEKVWEAQQALLSQNTDDLFSFASAFQYSAPLRGVACFP
                     QGKQFLFNEMIRTLESQGGLYLSGHAVKSLDRGKTITLEMEAPDKSTSRVAGRYLIVS
                     TKSEALPLITQKRGHAFFWDKFRKAGIAGYPFTLFLGVAQKFLPEQMARHMAVVADVH
                     RDLHDRNLILLETGMPEKGKPLPQAKNFMTATVYLPADDAAWAPEALRREAESILERL
                     DQFFPFLKDGIEMYDLDKSIELSLKYRKVFSPKYRVRNAFFTSFSAKNDRTRWSNVFL
                     TGTSLLADAGFEAEILSGRNAVSRVLQKGK"
     gene            complement(36186..36845)
                     /gene="purN"
                     /locus_tag="PLQ77_01945"
     CDS             complement(36186..36845)
                     /gene="purN"
                     /locus_tag="PLQ77_01945"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPL09472.1"
                     /translation="MADLLKLGVLISGSGSNLQSIIDHIEKGALGARITMVASNRPDA
                     YGLTRAQQYRIPTAVVSHRDFENREAFDRELLRILQGAGVELVVLAGFMRILTPFFLR
                     AFPDRIINIHPALLPSFPGTHVQQKALDYGVRFSGCTVHFVDEGVDTGPIIIQAVVPV
                     FAGDTAQELAARILKEEHKIYPQAIRYFAQGRIVRNGRRVEIRDAGNHDAALSNPPPE
                     G"
     gene            complement(36855..37922)
                     /gene="purM"
                     /locus_tag="PLQ77_01950"
     CDS             complement(36855..37922)
                     /gene="purM"
                     /locus_tag="PLQ77_01950"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPL09473.1"
                     /translation="MPTKSIKSTSSTYKESGVDIETANAFVERIKPLIKTTARREVLS
                     GIGGFGGLYRFDTAKARNPILVSSTDGVGTKLKIAQLTDRHNTIGIDLVAMSVNDVVV
                     QGAEPLFFLDYLATGKIETEKSVQIVEGIVQGCKQAGCALLGGETAEMPGFYPPGEYD
                     LAGFCVGVVESEKLIDGSTISINDRVIGLASSGLHSNGYSLARRVLLEKGGLSVKDSV
                     AELGRSVGEELLEPTRIYVKPLLNVFKSFQVKGLVHITGGGFYDNIPRVIPDVCRCVI
                     RKESWPIPPIFSVIQEIGRVDEREMFRVFNMGIGMMMIVPEKDVQEILDRLKGLGENA
                     FLLGTIEKRDENGPPVCFTDL"
     gene            38096..40558
                     /gene="recG"
                     /locus_tag="PLQ77_01955"
     CDS             38096..40558
                     /gene="recG"
                     /locus_tag="PLQ77_01955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPL09474.1"
                     /translation="MESFKKNLQRIEQPLNFAAKDQFKNLPNIRNLGKSLSGLIALQI
                     SAIPPAAKNIFGPALDHMMRIFQEYDGKDLQQKKSGIAEASLILERLKYAADNFDARN
                     PGNLQEEQIAARVEDLKISAEKLSAPVQFLKGVGPKMAERFSAKKIQTVEDLLYFLPR
                     TYEDRREIRKIHKLETNRIQTIMAAVADCSFRCYGKRRILEVAVSDNTGVLTAKWFKG
                     RMSHLAGAFQKGTRVIFTGNVTPGYAGKSMIHPDYEILDKEDEENLLNFRRIVPVYSE
                     TEGLHQKYIRKVMALALENYSRYIASPIPADICRKRGLINIREALVGAHFPGSDALME
                     PLQNARSEAHRRLIYDEFFFFQLGMAVKKSGRVLDRGISFAAGGSRLAKFYASLPFEL
                     TGAQKRVVDEIHKDLHAPTAMNRLLQGDVGSGKTLVAMSAMVTVCENGYQAALMAPTE
                     ILARQHEATLSAWAGPVGLRVVLLTGGMNGAARTEALERIGSGEADIIIGTHALIQEN
                     VDFRKLGLVVIDEQHRFGVMQRVALRNKGLSADVLVMTATPIPRTLAMTVYGDLDVSV
                     IDEMPPGKKPVRTLVMGENRREAVYEAIRKELAGNHQAFIVYPLVEQSENLDLKDATK
                     MAEHLQKDIFPNVRVGLIHGKMKDREKDAVMRDFQENQIAILVSTTVIEVGIDVPRAS
                     LMVIEHAERFGLSQLHQLRGRVGRRDIPSTCILLADYKASGDARKRLKIMENTTDGFV
                     LAEEDLAIRGPGDFLGTRQSGLPDFRIASIIRDARILNDAKEDAFALIVRDPLLAKPE
                     HAILKETLIAKWQGKLDLARTG"
     gene            40811..41116
                     /locus_tag="PLQ77_01960"
     CDS             40811..41116
                     /locus_tag="PLQ77_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09475.1"
                     /translation="MKTSRMIALFIAVVFLAFSTMAFAAGPGKKPDRVPPKLQSPLLG
                     SEMLWDRPEAFGPVPKSKRNLGKQVCGADSKAIGYHPKAQDEKGKVFPKGGFLCAPK"
     gene            41404..42321
                     /locus_tag="PLQ77_01965"
     CDS             41404..42321
                     /locus_tag="PLQ77_01965"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPL09476.1"
                     /translation="MRIAVVGIGGVGGYFGGKLAREYAASGKHEIVFIARGEHLRAIQ
                     KNGLQLCAREGQYTAWPNIATDDPSVAGRFDLVFFCVKSYSLEESARSLAKNIGKTTV
                     VIPLLNGVDSAERLKGVLPGADVVSGSVYIISFLDQPGVVIQTQGACRLIFGTDDRVS
                     ADRYSPVLELLLGAKIDARLSGRMTEALWMKFLMMCPLGSLTAATGKTYGLIRDDGDL
                     QNRLRAMMLEVAAVARARRVHLPDSAVDKTIELIGGFSHDAKTSMQLDRERGRQTEVD
                     TLTAFLCRAGREAGIPTPVHDEVYRQLTS"
     gene            42463..>43417
                     /locus_tag="PLQ77_01970"
     CDS             42463..>43417
                     /locus_tag="PLQ77_01970"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPL09477.1"
                     /translation="MKNISIGLIGLGNIGTGVVRLMKQNEKLIARKLGAKLVLKKIAD
                     IDINSPRGVKIPKNLLTTDAREILNDSDIAIVIELMGGYEPARTFVLEAIRNGKHVVT
                     ANKAMLATYGNEIFPAAQKQNVDIGFEASVGGTIPIIKTLKESLVANRIQSIVGIMNG
                     TCNFILTKMTNEGKPFDVVLKEAQELGFAEADPTFDIEGIDTSHKMAIVLSLAYGKKV
                     RLKDIYLEGITKISAEDIAFARELGYRIKLLAIGRLKGDAVEARIHPTMIASGHLLAN
                     VNHNYNAFHLEGDASGPVFLFGQGAGMMPTASAVFSDILDSA"
BASE COUNT         9615 a        11690 c        12470 g         9642 t
ORIGIN      
        1 gggcgcgtga agcttgacgt ccgtctcgat aacgagacag tctggctgac ccagcagatg
       61 atggctgatc tgtttcagac atccaaacaa aacataagtc atcatattca aagcatttat
      121 gacgaaggtg aactgcttac ggaagcaact gttaaaaaat atttgacagt tcgcgtcgcg
      181 caccgcgcag gcgcgtggaa tgaagctcgg attatattgt tggcatgccg atctgacacg
      241 tgttgtggcc cgatggcttg tatgccgacg gttcaagtac gctctagtat agcaaaggaa
      301 acccatgaat tgggtaatac ccagtaaatt aggtactaaa ttaggtaata agaaaaatct
      361 ataatgatat caatggacat gaaagccttc acttgtagca caaactggaa atctttagga
      421 cgtgttagtg gataaataac taaagatgat aatacccgat aaacgtgata aataacctga
      481 taataattag gaatcccaaa agatgaatat cacgaagaat atcaagaaga tcacgatgct
      541 tttgactgtt ttgatgctgg tcgctttggc aacgggctgc gtatcctggt cgaatacgcc
      601 tttgcatgtg ttgaggtatg atcaacccgg tcagcatgaa gaacggcggc tgattgtctt
      661 cctgcgcggc atgggaggcg gcaacgaaag ttttgaagag cagggatttg tggacgaagt
      721 cctggcgcgc gggctggcgt atgacatggc cgcacccgac gtgcatatcg gatactacat
      781 ggatcgcagc atgattcaaa ggctgaaaga ggacgtgatc gacccggcaa aggccgaagg
      841 ctgccggaaa atatggcttg tcggcgcttc catggggggc ttgggcgcga tcctttatct
      901 gaaggaatat ccggaggatg ttgcgggtgt gtatctgatg gcgccgtttc tgggcacgca
      961 gtcgattctg gatgaaattg atgcggcggg cggcctgagc tgctggaagc ccggcgctct
     1021 tcaggatgat gacgattggc agaggatgct ctggacgtgg ctcaaaacgg aggtggccgg
     1081 acgcccggaa aaaatcgtgt acctgggata cggcacggaa gatttttacg ggaagggagc
     1141 gctgctgctg gccccggtct tgccgccgga ccgcgtttat gccattgccg gcgggcatga
     1201 ctattcgacc tttaaagccc tgtggaaaat gttcctgtcg ggaagcgctc cattgaggga
     1261 tgaaccctga atgtagtccg tgccttcagg cccggtcagc ttcaggccct gagtggttgt
     1321 tcaggattat agaccttttt atggattccc gccttcgcgg gaatgacaaa agagctatgt
     1381 agggcgtacc ttcaggtgcg ccggaaagct ttaccttgat tctattttcg cagtaatgat
     1441 gccggccaga tgcttcgccc agaaggagta ggcgcgggtt cccgggtgaa agccgtcgtc
     1501 ggccatatag gttgtctcca ggggaaaatc aatgcttaca aatgtgcacc ggctgcatcc
     1561 ctgaacggat tgctccagcg cgtggttgaa atgcttggcc ctgctgccca gataccagcg
     1621 caggggctgg ggcagggcgg gaaaaagatg catgggggga atgccggata aaatgacatg
     1681 cggtgaatca aatttggaaa gaagcaggcg aatgattttt tcctgccttg caatccaggt
     1741 ttgcctgccg gtcccgtgag tgacgtcgtt gacgccgatg gaggtgacca ccacgtcgag
     1801 tttctccggc ggtatctcct ccagcatggc cagaacgtcc tttgaccggt ggccggtgcg
     1861 ggcgatcagt ttccaggaca cgcggaagtt ctggccgagc gcgcggacca gttgaccgga
     1921 aagggcctcg ctctgccgcg agacgccgac gcccgccgcc gcggaatcgc cggcgataag
     1981 cagtttgagc ggcgggccgg agccgtgctc gccggatcgc tcccccagag gctcgggcag
     2041 tctgggggtc acgcgccgca cgtagagggc ctgggcgatg aggaccggcg caagactgat
     2101 gattgcaagt gagtttctca tgacgaacct ccggcggggc cgatggcttg aaggatgctc
     2161 aagagttacg gagtcctcag gtagaagccc agatccgcgt ccatggagtt gggcggctcc
     2221 tggaactggc agccgacata tttcttgaag atggagcgga cggcggcctg ttttttaatg
     2281 acggaacgct ttgcatcatt gagctgaaat tcaattttga tcaaatcccc cggctgaagc
     2341 gggttgacgc ccatcgtttc aaaaccgcat ccctgcatcg agacgttttt caaaaccatt
     2401 ttcccccaga ttttggggtt ggtcactccg aagtacaggc cgtccacccg gattcccttg
     2461 cggtagaatt tccgaaactc gatttccacg tcatagacgt ttccgcaggt gcattgaact
     2521 tgaatcgggc ctttggcgcc tccataggtt tttgcttcct gcttttgtat caaattgcat
     2581 ttggagcaga cgaacacaac aaatccttcg ttgtccacgt acactttcgt aatggttttt
     2641 tccatacgcc atcccctcat cggtttcttg acttaggtaa gcacagtata aaatggcccg
     2701 aaaagaaaaa aacccgtctt gcgattctga acatcgcaaa acgggttcca tcgtccaaat
     2761 cgatttattc cagaacggcc agcacctggc cttcattgac ggagtctccc accttgactt
     2821 tgatttcctt gatggttcca gcgcccgggg catacacggg gatctccatt ttcatggcgt
     2881 ccatgatgat cacttcctct tcttcctgca cctgcgtgcc cacgttcacc gcgatgctgg
     2941 cgattttccc cggcatgggg gccgtaatat ccatactcat aatttttccc cgcctccctt
     3001 tatcgatatt ttttgtctca caaaatgaac gccaaagcct gcgccgtcca agccgccgct
     3061 ttgaaattgt tgaagctttt accgtaaatg cgggttcagg tcaatacaat tattcggcgg
     3121 cgggcgcgtg accgctcatt catgccgcag agcgtcgatg gggttcatca aagaggcctt
     3181 gtaggccgga tagaagccga aaaagatgcc gaccaggccg gagaatccaa aggagagaag
     3241 gatggagtag gttgaaatca ggattgtcca gccggcgaaa aaggatagaa gctccgcgaa
     3301 cagaatgccc agcaaaacgc cgatcaatcc gccgatgagc gagagaatca tcgcctcgat
     3361 gatgaattgc aggcggatgt cccaggtttt ggcgccgacg gccatgcgga tgccgatttc
     3421 cctggtgcgc tccgtgaccg aaaccagcat gatgttcatg atgccgatgc cgcccaccag
     3481 aagcgaaacg cctgcgatag cggcgagcag gagcgccatc acgcgggaag cctgctccgc
     3541 catctgcatc atttgcgtca gattgcggac ggtgaagtcg tcttcctgat ccggaccgat
     3601 gcggtgccgt tgccgcagga gctccttgat ttgctgctcc gcccggtcca gatcctccgt
     3661 gcttctggct ttgaccataa tcgagcgcac cgttcccgga aaccatcgtc cgaagaggtt
     3721 ccgctgcgcg gaggagaccg gcacatagac gatgtcgtcc tggtcctgcc ccagcatcga
     3781 ctggcctttc ggggccagaa cgccgatcac gacaaacggg acttttttga tgcgaaccgt
     3841 cttgtggagc gggtcgtcgt tgccgaaaag attgtccacg acggtttgtc ccagcaggca
     3901 gattttggcc gcgctgcgga tttcctgatc gtaaaagttc cggccggaag ccagtgtcca
     3961 ttcccggact tccagcatgt tttcatccgt tccgaagacg atggagggcc agttggaatt
     4021 gccgtaaata atctgggcgc tgccgttgag gatcggggcg gtcgtggcga ctgcggagca
     4081 ctcctgtttg atggcctcgg catccgagcg ggtcagattc tgtgtggcgc cgcttcccaa
     4141 acggatgccg ctggcggtgg tcgaacccgg aaggacgatg atcaggttgc tgcccacgga
     4201 ggcgatctgc tcggatatct tattgctggc gccccgaccg acggccagca tggtgatcac
     4261 ggcggccacg ccgatgatga tgcccagcat ggtcagcgcc gaacgcatct tgttggcgcg
     4321 cagggcgcgc agagaaattt tagcggtgga aagaaagttg atcatcgttt gtttaccgag
     4381 tcgctgacca gttttccgtc caggaaatgg atgatgcgct tgctgtaggc ggaaatgtcc
     4441 ggttcatgcg tgaccaggac aatggtgata tttttctctt cgttgagccg gacgaagagc
     4501 tccatgattt cgatgctggt tttcgtgtcc aggttgccgg tcggttcgtc ggcaaggacg
     4561 agcggcgcgt tgttgaccag cgcgcgggcg atggccaccc gctgctgctg accgccggaa
     4621 agctggttgg ggtggtggtg gcgcctgtct tccaggccca gtgtccggag cgcatccaat
     4681 gcccgctccc tgcggttttt gcttccgtct ttgttgtaga gcagggggac ttcgacgttt
     4741 tccagcgccg tcgtgcgcgc gagcaggttg aatccctgaa agacaaatcc gattttttca
     4801 ttgcggattt ccgcgagcgc gtcgcggctc atagaaccca cgtcgatgcc gtcgaggcgg
     4861 tacgttccgg ccgtcggcac atccaggcag ccgatgacgt tcatgaaagt ggatttgccg
     4921 gagcccgagg gccccatcac cgccacgaat tctccctgat ggatctgaag ggaaactctg
     4981 tccagcgcgt gaacgatgct gtcgccgact tcgtatcgct tttccagatg gtccgtctcg
     5041 atgagcgcca tggccgttag aacctcggtc cgggccgcgg ggtctgcgtc gccgcgtttt
     5101 tctggtcgtt gcttttgact tccacgatga ccggcgcgcc ctgaagaatt tttccggaga
     5161 cgacttccgt gaagcggttg tcgctgatgc cgatggaaag gggaacgcgt ttgggctttt
     5221 tgttttccag cacccagacg ccggggcctt tcggcgccgg agcggaggag gatttgtcct
     5281 ggattttcag cctcagcgca gcgttgggaa cgcgcaggat tccttttttg tcgtcaatga
     5341 cgatcgagac gttggccgtc atgcccggct tgagcttcaa atccgaattg tccaccttga
     5401 cgatcacgtc ataggtgacc acgttctgca ccgtggtggg cgagttgcgg atttcggaaa
     5461 cgtttccccc aaacgtcagg tcggggtagg cgtccacggt gaaggtgacg ggctggccca
     5521 cgcgcaggcg gccgatatcc gcttcgtcga tgttggccac gatctgcatt ttggtcaggt
     5581 cctgggcgat ggtaaacagc gtcggcgtct gaaagctggc ggcgacggtt tgcccgacgt
     5641 ccacgctgcg cgagatgacc gttcctttga ccggcgaaac gattcttgta taacggagat
     5701 tcgtctcggc gaggttcagc gcggcccggg tctgctccac ctgcgcttcg gaggctttga
     5761 tctgggcgag cgcggacagg taggcggtct ccgacgtgtc caggtcgtgt ctggaaatga
     5821 aatttttgcc gtaaaggatt ttgttccgct caaatacgga aaccgtgtcg cgcagggcga
     5881 ctttggactt ttccaggttg gcgcgggcca gcgacagatt cgcggcggcc tgggccacct
     5941 gcgcctcgat cgaagccgga tcgatccggg ccaaaagctg gccctgcctg accgtggagt
     6001 tgtaatccac gtaaagctcc ttgatcgttc cggacacctg cgtgccgacc agaacggtcg
     6061 tgacggcatt gacggttccc gtggcggaaa ccgtcgcgcg gatgtcgccc cggtccgccg
     6121 tctgggtgat atactgcggc gccggctcgc ggcggcttaa aaagaaaaac agggcgatca
     6181 gaatcaggat tagaatgacg ctcgcggcaa tgattttttt catggcattt ttgctcccat
     6241 ggctttctcc aggctggctt cggcgatgtt gaaatcggac agggcggata tgtaggtcat
     6301 tttggcattg ttgagcttga tcatggcatc cacgatttcc agggaattgc ccacgccggt
     6361 cgcgtagcgc ccctgcgcaa gctccagaga ttcttcggcc tggcgcacgg tgattttgcc
     6421 cgcctcgatc cgttcaaaag cctcgtggcg gttgagccag gcggacttga cttcctgaaa
     6481 agccttctgg cgaagcgctt cctcgttggc tttggcaatt tccaggccgg cccgggcctc
     6541 ttcgacggcg tatttggtgg agaagcccgt gaagaaagga aaggtgaggg cgacgccgac
     6601 gttccaggac tggttcaggg cggcgtcctg acccgcgtag ccgtagccgg cgcttccgga
     6661 aaggacgggc agataatcct tcttgttgag atcgacggtt ctttccagcg cgtctttttt
     6721 cagagcgatc gactggatgt ccggccggtt ttcataggca cgggcgaggg cggcttcaaa
     6781 cgtgatgtcc gcggcgtcca tattgaactt ttcctgaatg tcgtattcgg gggcgttaat
     6841 cacgcccatg gccttgttca ggttcacccg ccaaaggcga agggcgttct gggcggaaat
     6901 caactgaatg cgggcgttgc tcaaattgac ttccgcgctg gtcacgtcga tcttggagct
     6961 tttccccgtt tcaaaaaatg tctgggccac ttcaaaatgc tgctgaaact ggttgacggt
     7021 ctccacggcg acggtttcgc tcatcttggc cttgagaagg gaaaagtagg cttcctgaac
     7081 attgtaggtg agcaaagagg tggtgtcctg aaaatccgct tccgccgact gcttgcccag
     7141 cttctgaatg tccacggcgg tggaggtttt gccgaaatca aacagggtct ggttcagatt
     7201 cagcgcgttg gagtaatagt cgtaggagct gttggtcaaa gacgacgcgg gcgggccgat
     7261 gcgcgagtag ccggactgaa aggaaagctg gggatagtaa ccggcgcggg ccttgccgat
     7321 tttgctctcc gactggtgaa tggctccggt ggccgcgttg agggccggat gatttttcag
     7381 ggcgatgtcg atgcactgcc ggagcgttag gacatctccc ttttgaatgt cacccgccgc
     7441 gaacagactt tgggccggga tgaacaacag cagaaacaga aggaaaattc ttttgcgcat
     7501 agaagatgta aaaaaccctt tcaaatcgta aatccctctt tcccggattt cataccacaa
     7561 atcatttctc gttcacacct atatcaattt ttgtgccagt aatcatggtg gatagtgaat
     7621 cgtggatggt ggatggttaa atgtagggaa cggtcgcgac cgttccctac gggtaatagg
     7681 tcatgcttgt atcttccatc gcttgtgtca tgctgagcca gtcgaaacat gacgctttcc
     7741 gctcaacacg cattgctttt tgccccgcac ccatcacctc taaccccggc ccccttacct
     7801 ctcatccaac aacccgctgc atcttatttc cccacctttc gtatccaatc gggtaatctg
     7861 ttcccattaa aaaatctgaa ttatatttgg ccgataattg gcaataaggt aattcaatgt
     7921 ctgattttgg aatattattc ttgattaaat tttcagaatg cattacgaaa tcaggcgcaa
     7981 aatgaaacgt gaaaggagca tgaatatgag gatttttgtc agaaaatggt cggcattggt
     8041 tgttttgctt tgcctggttc tggtggccgg atgcgcatcg ctgtcgtccc agaaaagcga
     8101 tcagttcatc tttttgtcca gtacgatcgg cccgatcgat tccggcattg tgggggcgct
     8161 ggaagatcag tttgaaaaag agacgggaat ccgcgtccgc cacgtcggcg cggggaccgg
     8221 tgcggcgctc aagctggcca ccaagggcca gattgacctg gtgatggccc atgcaaaatc
     8281 gctggaggag aactttatcg ccgacggtta cggcacggag agaatcccgc tcatgtacaa
     8341 cgattttgtg attgtcggcc ccgcatccga tccggccgcc gtcaagggag aaaaatcggc
     8401 gacggcagcc ttgaagaaaa tcatggacgc aaacggcaaa ttcatcagcc ggggagacaa
     8461 gtcgggcacg catgtggcgg aaatggaact ctggaaaaaa acaggtgtcg aacccaaagg
     8521 cgcatggtat gttatttatg agaaggggtc cgaaggaaat gtcgccacgc tgaaatacac
     8581 cgatcagcag gccgcttaca cggtgatcga ccgggccacc ttcctgtccc tgaaagatca
     8641 gatcaaggtg gtcattctgg tggaaggcga cgaggcgctg ttgaattaca tttctttgat
     8701 accggtcaac ccggccaaat tccccagggt caaccgcaag gacaccctga cgtttgtcca
     8761 gtggctgacg tcgccggaaa aaggccagct gatcattcgg gatttcggca aagaaaaata
     8821 cggatcgccg ctgtttttcc ccaattccgt ggaatggcag aaactgcaga aaaaataatc
     8881 atcaatctga aggagacaga atatgaaaat gttcaaaagc agcaagtggt ttatcatcct
     8941 ggccgttgtc gcggctcttg tcatgctggg cgcggcaggc gcgtttgcgg cacccaagca
     9001 gaaaaacatc attctggcca caacaaccag cacgcaggat tccggactgc tcgacaccct
     9061 gatccccatc tttgaaaagg agacgggcta cttcgtgaag acgatcgccg tcggttccgg
     9121 acaggcgatg aagatgggag aaaaaggcga ggccgacgtc atgctggttc actcaccgga
     9181 tgccgagaag aaatttgtcg aagcgggcta tggaatcaac cgccgcctcg taatgcacaa
     9241 tgacttcatt atcgtgggcc cgccgaacga tccggccggg atcaaaggcg tcaaatcctc
     9301 gtcggatgcg ctcaagaaga ttgccggcgc caatgctctg ttcatctcgc gcggggacaa
     9361 ctccggaacg cacgcgaaag aaaaaaccct gtggaaaaag gcaaacgtca atccggccgg
     9421 acagaaatgg tttcaggaaa cgggtctggg catggggcag acgctgaatg tcgcctccga
     9481 gaaaaaagct tacacgctgg cggaccgggg cacattcctg gctatccgga aaaacctggg
     9541 cctcacgatc ctgaatgaag gcgacgcggc gcttctgaat atttatcatg tgattgaagt
     9601 caacggtgca aagtggccga aagccaacac ggaaggcgcc aaagcgtttg cggattttat
     9661 ggtgtccaag aaaacgcagt ctatcattaa aacgtttggc gtcgataaat acggttcgcc
     9721 gctgttcttc ccggatgccg gcaaaaaagt ggaagattta gggaaatagc gttcacaatg
     9781 gatttgattg ttcaaggcgt catcaaagcc tttcaattga tcatcagttt tgatcctgaa
     9841 gtgatgggaa tcacgtggct gtccctgaaa atatcgggga cggccacttt catcagcctg
     9901 ttcttcggcg tttccgtcgg gacggccgtc gcgttgaaca actttcccgg aaaaaggctg
     9961 gtgatcagca ttatcaacac aggcatgggg ttgccgcccg tggtggtggg cctttttgtg
    10021 accatcatga tctggcgcag cgggcctctg ggttttctgg aaatcctcta cactccgtac
    10081 gcgatgatca ttgcgcaggc catcattgca acgccgatcg tcatgggcat ttcgctcgcc
    10141 tcgattcaga atctgccgcc gaatctgcgg ctgcagattc tgtcgctggg cgccagccga
    10201 atgcagatga tctgggtgct gatcaaggaa tcccggcttc cgcttctggc ggccgtcatg
    10261 gcgggttttg gaggggtaat ttccgaagtc ggcgcttcga tcatggtcgg cggcaatatc
    10321 aagggatatt ctcgcgtgct caccaccgca acggtgatgg aaaccggaaa ggggaatttt
    10381 gacattgcca tcgctctggg cattatcctt ctgatcctgg catttctggt caatcttctg
    10441 ctcacgcaaa ttcagcaaag gcagcatccg agatgaacaa catttttacc atagaaaatt
    10501 taattgtccg ccgcggcggc gtcaaagtgc tggatatcaa cgagctgaag gttcacgaaa
    10561 gcagaattct ggccctgatc ggacccaacg gcgcgggcaa atccacgctt ttgctgacca
    10621 tggcgggcct gctcaaaccg gatcagggga aaatgcatta caagggacgg cccgtggagt
    10681 cgagcgcgga gcgctcccgc atgcgcagga gcgtgtgcgt tgttttccag gagccgctgc
    10741 tgctcagcac gtccgtttat aaaaacgtgg ctctggggct gaagttccgc ggaatgtcga
    10801 aaaatgacat tcagaaaaac gtcggagatg cgctgtcgta cttcggcatc agttcactgg
    10861 cgaaccggtc ggccaaaacg ctttcgggcg gcgagtccaa aagggtgtct ctggcgcgcg
    10921 cctttgccct caaaccgcat ttaatcctgc tggacgaggc cttcaactcc ctcgatccgc
    10981 cgacgcgcga gtcgctgatc gatgatctgc ggcatattct ggaagagacg aagatcaccg
    11041 ccgtgctggc gctgcacgac cgggaggaga cgctgcggct ggcgcaggat gtcacggtga
    11101 tgtctgaagg acggctcatc cagaccggcg ccacggctca aatcttcaat cagccccagt
    11161 ccgagttcgt ggccaatttt gtcggcaccg aaaccattct ggaggggacc gtccaaaaca
    11221 tccggtcggg tctgatggaa gtgctcgtgt cgggaaaagt gatcgaggcg gcaggcgcag
    11281 ctccgctggg acagaaagta ttttgctgca tccggccgga aaacatcgtc gtatcaaggg
    11341 aagcgccgga aaatacgagc gcccgaaaca tctatgaggc cagaattcag aagattgtcc
    11401 gccaggcctt ttcctacaaa ctggtcctgg actgcggatt tcccgtggtt gcctatctta
    11461 ccgtttcttc ctgcgaggat ctggggttct gcgaaggcgc cgcggttttc gcgtcgttca
    11521 aggccacggc cgttcacgtc atgcgccggg aaaaatagac cggcgatgca gctgaaaaaa
    11581 agcggccgca gccgcccttg cgggcggcat gaaaccgctc ttcatccatt ttgaaaaatc
    11641 ccgattattt catggcctgt tcaaccgcga cggcgacggc gaccgaagcg ccgaccatcg
    11701 gattgttgcc cattccgatc aagcccatca tctccacgtg cgcgggaacg gaggacgaac
    11761 cggcgaactg cgcgtcggag tgcatgcgcc ccatcgtgtc ggtcatgccg taggaagcgg
    11821 gaccggccgc catgttgtcg ggatgaagcg ttcttcccgt tccgccgccg gaggcgacgg
    11881 agaaaaactt ctttcccgcc agcaggcgtt cttttttgta aacgcccatc accggatgct
    11941 ggaagcgcgt cggattcgtg gaattccccg tgatggccac gtccacgcct tcgagatgca
    12001 tgatggccac gccttcgcgc acgtcatcgg cgccgtagca gcggacggcc gcgcgatcgc
    12061 ctttggaata agctttttcc ctggccacgg agactttccc tgttttataa tcgaaagccg
    12121 tcgcgacatg ggtaaatccg ttgacgcggg cgataatgtg cgccgcatcc ttgcccaggc
    12181 cgttcagaat gacgcgcaga ggattcttgc gagccttgtt ggcggacttg gccaggccga
    12241 tggcgccttc cgccgcggcg aatgattcgt gtcccgcgca gaaggcgaag caggcggttt
    12301 cttcgcgcag cagcatggag gctaaattcc cgtggccgat gcccacctga cggtcgtcgg
    12361 cgacggatcc gggaatgcag aaggactgaa gtccctcgcc cagcgtctcg gcgatgtccg
    12421 cggcgttttt ctggcccttt ttaatcgcgg ctgccgcgcc cacaagatac gcccatccgg
    12481 cgttttcaaa tgaaatcggc tgaatatctt ttacaatttt ccgaacatcc acgccttttc
    12541 cgatgcacac tttttcggct tcctccagag aggaaattcc atatttggcg agaaaaggag
    12601 tgatctgttt tattcttctt tcgtatcctt caaatagtgc cattttaaaa ctccttatcc
    12661 tttacgtggg ttgatgactt taacggcttc ggcaaagcgt ccatacgttc cgcttgcctt
    12721 agacagcgcg gcggccggat ccatcccttt ggtaatgtta tccatcatga cgcccagctg
    12781 aacgaatttg tagccgatga tttcgtcgtc ggcgtcgagg ccgatttcgg tcacataacc
    12841 ttccgccatc tccagatagc ggggcccttt tactttggtg ccgtacatgg tggcaattac
    12901 gcttcggagg cccttgccca gatcttccag acccgcgccg atgggaagtc cgccttcgga
    12961 aaacgcggtt tgcgtgcggc cgtaaacaat ctgcaaaaag atttcccgca tggccacgtt
    13021 gatcgcgtcg cagacgaggt cggaattcag cgcttccaga atggttttgt tgggcagaat
    13081 ttcggcggcc atcgcggcgg aatgggtcat gccggtgcag ccgatgcatt caaccagcgc
    13141 ctcttcaatg atgccttttt tcacattcag ggtcagttta caggctccct gctgcggcgc
    13201 gcatcgcccg acaccatgcg tcaggccgct gatgtcctta atttccttca cgggcgtcca
    13261 gccgccctcc tgcggtatgg gcgcggggcc gtttttagcg cccttggcga ctacgcacat
    13321 ctgttcaatt tcgtgagtgt gctccataaa aactccatct ccttccggta aaatattggg
    13381 gataacggtt ggttatcctg tttttcttcg cactgaacgg gaaaagtccc tttacgggtg
    13441 gttcagaagc cgtcaagacg ggaagaatct attcaaaaag aaaatattag tcaatatgat
    13501 tattgacatg gcgccggata tttttgtccg gacctgattt ttttgagccc tccgaatctc
    13561 atcgcgcaaa ggcataagca ggcggaaaaa cattggcaat ttccccaaac aggaattata
    13621 agtcttcagg attgatgcgt catggccgaa aataaaaaaa gggaggggct atcgtgaatt
    13681 accaacttta cctggaaaaa attcgcgcgg gagagaaagg gttgaatccc tatctggacc
    13741 acttcaatgt agaggttgcc ggtttgtctt cgggccgcgc cgtatttcga atgcccatac
    13801 gtccggaata tatgcagggc gccggattga tgcagggagg tttgatggtc gcgctggcgg
    13861 acgaggccat tgcccatgcc atgatgacgg tgctcacgcc ggaagaagga ctgacgacaa
    13921 ttgagctgaa gagcaatttc ctggccggcg tggacaaagg agagcttatt gccgaagcgg
    13981 gcgttttcaa gaagggccga agcctgatca tcggagattg ccaggtgaca aacggcgcgg
    14041 ggaaacccgt ctgccgcgtt tccgcgacgt tcatgctgct gaggcagcat cagaagtgag
    14101 gcgggaaatt atatgcaatg cccgtcagtt ttcgggacac cgcggtccgg gactgatcag
    14161 gcgagaagcc cttcgacttc gccgcgaatc tcctgaaggc gtgccgcgga tgatgcctca
    14221 aagcgaagga cgatgaccgg ctgcgtgttg gagcagcgga ccagcgccca gccgtccccg
    14281 aaaggcaccc gaacgccgtc aatgtcaatg acgcccggcg tgttttggta atgctccttg
    14341 attttggcca ccaccgcttt cttccggtcg tcgtcgcaat ccatgcggat ttcaggcgtg
    14401 gcaaacgttt tcggaacgcc ggcgaggagc tcgctcagct tcagacctgt ctgagagaga
    14461 atctccagca gtcttacagc ggcgtaaatg gcgtcgtcat atccgaaata gcggtcggca
    14521 aaaaacaggt ggccgctcat ttctcctccc agcagggctt tttcctcctt catcttggcc
    14581 ttgatcagcg aatgcccggc cttccacatc agcggccggc cggaatattc cgcaatgccg
    14641 tcgtagagaa cctgcgagca cttgacttcg ccgatgatgg tcgccccggg atggtccttg
    14701 aggatgaagc gcgcaaacaa aagaagcagc ttgtctcccc agatgatctc gccggtgtcg
    14761 ctgatcactc cgatgcggtc cgcgtctccg tcaaaggcga tgcccaggtc agccttttct
    14821 ttggccgtaa gccgaatgag ttccgcaaga ttttcttcga cggtggggtc cgggaagtga
    14881 tggggaaagc ggccgtccgg ctcacagtag agggcagtga cgtcgcagcc cattctctcc
    14941 agaatgggaa gggcaaattt ccccccgacg ccgtttcctc cgtcgaggac aatcttcagc
    15001 ttttttgata cgcggacgga gtcgaagata tagtcctgat aaatcttcgt gatgtcctgc
    15061 gcctgcgcgg accctttgcc ggaatgataa cttccggatt ccatgatgcg cctcagctcc
    15121 tgaatctgct ccccgtggat ggaatcgtag ccgatgcaga ttttaaagcc gttgaactcc
    15181 ggcgggttgt ggctgccggt gatcatgacg ccgcctcccg ctcctaagcg gcggatggaa
    15241 aaatacagca tgggggttgc gcacaggccg atgtcgatgg tgtccatgcc ggaggcgttc
    15301 agcccggcga tcagaaatcg gtggtagtcg tccgaactca ggcggcagtc gcgcccgatg
    15361 gtcatggtcc gaactttatg ctgaagcgca tatgtgccga tggcgcgtcc cagatttacg
    15421 ataaagacct cgttgaggtc cttgtcaacg atgccgcgca cgtcgtattc acgaaagaca
    15481 tgtggattca ttttttccct caaaatcaaa gaagatgatg cgtccgggct tccggcgccg
    15541 tccgcttcta tacaatttat ccggggatgt caacccggga aggtcgttta aaaatcttca
    15601 ggcttcagcc gcctgagaat gtccggggct tccctcagca ccatgtccgc tcccgcccgc
    15661 agaaaatcct tttcctgaaa gcggccggtc aggacaccgg cggttcgggt tcccgcgttt
    15721 ttccccgtct caatgtcgat cgggtggtcg ccgatcatca cgcatcggcc gggttttgcg
    15781 ccaagcctcg acagggcgag gctgatctgt tcggggtgcg gcttgacgcg ccggacgtca
    15841 ttgcggcaga cgaccaccgg acaataatcg tgaatatccg gaaagacggt ccggacggct
    15901 ttctcacagt tgcgggtgat gattccggcc tgaagggagg ctttcctgag acccgtgaac
    15961 agctctttgg cgccgtcaaa cagggagccg ctttgcgccg cttccacttc aatctcttcg
    16021 atgattcgaa aggcttcccg cgtgaacgaa gctgcatggt ctcctgaatt ttttccaagc
    16081 agggcctgaa cttcgccgat catttccaaa acaaaccggt tttgcagact tcgggggtcc
    16141 acgccccagc ggtcaatcag tgaggccacg tcccggcgca tttgttcaaa atcgatgttc
    16201 agccgggcca gcgttccgtc aaaatcaaag atgacggtgt cgatggagga ccggtcgaaa
    16261 ggaatagggg ccatgggttc aacggtttcc cgacgtgtag tgtgcagaga gggttttggt
    16321 gacgatgcgc ccgggcgttt cggccggaaa agcgccgccc gtaacctccc gcaggaaatt
    16381 ggtgatgatg aaaaacgtgg cgccccagag gatgtcttcg ccgcccttgc cgtccggaat
    16441 gaccaggcag gggaattgga agttgcggcg cgggtcggat gttccgttct ccgggctgag
    16501 ctccaggaga gcgtaggaag agctgcggaa aaaatcgctg acgggaattt ccagcatttt
    16561 gtccacttcg tcgttgggcc ggtagggcca gggctccctg accaggcctg ccagcggaaa
    16621 gatggtccgg gtgaaaagcg tgagcgagta ggtgggcagg gcgccaagaa attcaacgtt
    16681 gagcgggctc aggccgattt cctcccagga ttcgcgaagc gcgttcatca gaaaaagccg
    16741 aatgagcgat tgggtctcac cgtctttgtt aggcagttcg tgaagcgccc ggttgcctgc
    16801 cgttcgaaga atgccggttt tcgaaacgat gtggctcagc atctcatcga ccggccgctc
    16861 cagcatgccg ccggggcaac tgatgtcgcc ggactgggcg acggcattcg aacgcttgat
    16921 cagcaaaaaa tgaaattcgg aatttttgaa cagcagcagc agcacaacgc cggcttccaa
    16981 atgtttgccg cccgcggcgt tggagcgttc aatcgaaagg tgcttttcca aatagtcaac
    17041 gggtgtccgg ttcaggcgct ccagaacata aagcggaaag gcgtccttgt cccgcagcag
    17101 cgttaagtcc atggtcatgc gtttctctct tgttgaaatt cttcaaaaat ctgttcattg
    17161 taccagcgcc gccggggaaa caggtccaca aaaccgcagt gaccgccgaa cggctgctcg
    17221 gaaaccgtca gaaaaccgtg attgcgaagc ttccggaagt catcgagggc gatgacggga
    17281 tcatcggccg cgaagaatat tttgaccggc acgccgagat cacaaaaaga attatccgtc
    17341 agagtgtata gccgaaaata gtcccgatag gaaggaaatt ccgggaaata agccatcatc
    17401 ttctccgtca gctccatgca ggaacgcgcc cgaagcatgc cggcaaagtc gtattgatcc
    17461 gggaaaaacc tctgtttttt tctgagcgaa cgcttccact ttcgcagaaa gtagttccgg
    17521 taaagagcgg ggccgcggtc gatggtctgc gtcgttctgt aagggtcgag gggcggactg
    17581 acggcgaaca ccttttgcag gtttccgacg ggggtccggg aatggcgcat cgcgatcctg
    17641 agcgagaaat tggcgccgag ggaaaacccc aggagataaa ccggcaggtt tccggccatt
    17701 cttgcaaccg cgtttaccgc gtcgaaggtt tcctcgatca gcgccccgtg aaagaggcct
    17761 tgattgaggt gatgggaatc gccgtgatcc cttaaattga ggcggcaaac ggaaaaattc
    17821 cggtcgaaaa agtaggcgcc ggcggccaga atgtaggccg aagaggagga gccttcccag
    17881 ccgtgcagca gaatcatcat tccccgggaa cgggggtgat gcgacagata ggacagcagc
    17941 ttcgatccgc cgggcgtttc gatgatgaat tccctggaat gcctgcgcag ggggccgtga
    18001 gggggaatca tcaggtgtga agacgccaga acggactgca agtgccggtt cttcaaaaaa
    18061 gaccggggtt gaaaggttgc agaagggttt ccgtttccgg cagtcatgag tttcccgttt
    18121 tattaaaatg atttgcaata tccaggcgtt gacatcgcgg aaggatgaaa tggtggcggt
    18181 gcctggattt gaaccaggga cactacggat atgagccgta tgctctgacc aactgagcta
    18241 caccgccttg aaaagcaagc gttctttgcg aagccgccgg aggctatacc gaagagtccg
    18301 gcgtttgtca atacctattg cggcatttcc agttcataca cgaccagaac gcttctctcg
    18361 ctgagcgtcg cgccgacgga ctggaccagc gccaggacaa tttccggttt gccgtcattg
    18421 tccacgtcct tgaagcagta gtccgcgaca tagccgttga ttttcttcgt ccgccagttt
    18481 tccgccatgc cgaggccgtc ccactccagg ttatagattt cgctcgagga gaaaagcttc
    18541 aggtttttaa agatgcgtcc gaccgacgaa aggtttttga cgatgatcat ttcctttttg
    18601 ccgtcgttgt tcgtgtcgaa catcacgatt ctcagattgg caaaaacact gtcctcctgc
    18661 gtggcctccg aagactgctg cttctgcttg tccacgttgt cgatgtagtt gttgctgccg
    18721 ccgtactggg agtcgcttct ccagatcagt tcatcggggc tgaatccaaa ggaaaaaatc
    18781 cggctcagcg gcttggtcgt cggcgtaatg atgcacaggt agtccagctc gtcgagcgcg
    18841 atgattttct cgctggttcc cgcgcccagc gtgtcaaggg tcagcccgta aatagacaat
    18901 ccgaccggga ttttctgctt atccccctcg acgtatttcc cgtcccgcca gacgatttca
    18961 taaatctgcg tatcgaaaac ccggccgccc tggccgtcgt gaaagcttcc cagtccatag
    19021 gcctgcccca gcagaacggg cgcgcccgag gaagggatga tcaccctcag gaaataacgg
    19081 atatcggagg caattgtttt gtagtcgccg tcctcgtact gaagaacgaa agaattgagc
    19141 agccggtcgt tgagcgacgt cacgatgatc tccggcgtgc cgtctttgtt gatgtccgcc
    19201 acgtccacgg agatgtattt atggtaggat tttcccttga tctgcttgag cagctgcaag
    19261 ttgtccttgg tcttcaggta aatatataca ttgtgggggt caatgatgac tacttcgttc
    19321 cgaccgtcgc ggttgacgtc gccgacgtcc atcccgttga attccgtctt gaactgctgg
    19381 ctcatccaga agcccttctt gcgatcgagc ggatcggacg aattgatgaa ttccgcgttg
    19441 atcatcgaag tcagggttcc tttgcctttt ccggatttca tgccggcgat gatctgcgct
    19501 tcgcgggacg cgccgggaac aggcggcggg gcggcggccg gcgcggtgga caccgcagcc
    19561 ggagcgcttt ccgcggcggc ccctccggtg atatgccgga cgatgcgctg cgaaaaatcg
    19621 ttgattttgg gaatgacttc gtcgagggtc tgggactggg agaaaatgcc gacgtccgat
    19681 ttccctccgg tgatgtcgat cagctttccg tcgatgctga tgctttttcc gatcttggta
    19741 atgctgcccc agaccacgta gtcggaattc aactgccggc cgatgtcctg aacatccgcc
    19801 aggccgagct cctttccgcc tgttttcttc aggacgtccg aaacgacttc tttgcgcgta
    19861 acatcaattt tgtcggctcc ggaaatccgg ctgatcagca tgtcgttgat tccctgacgc
    19921 acatattcaa tgttttcagc gctgtgcagc gcgaagggca gaattgtcac cgtgtatttt
    19981 tcctttgccg tgagcgtcgc cgccatcagc agcgccgcta gaaggcaaaa accgatcatt
    20041 atttttttca ttttttcact ccgttgttga cggcttttga taatggaaac tgcctgtgct
    20101 tctagcactt aaaaattgat aattcaaggg tggttttcag cgcggtgggc ggcggcgggg
    20161 acgggaaaaa gaatttagaa ataaaaaatc gtgttgtcag atcagcgcct ttcatgtaaa
    20221 gaaacattta caaaacggct ggatgggccg gggtttgcaa ggggtgaagg acaaaatgga
    20281 aaaagcaacg ctgaagctca atggaatgac gtgcgagctg ccgatcgttg agggaacgga
    20341 aaacgaaaaa ggcatcgaca tatccagact gagacaggat acgggtctga ttacactgga
    20401 cgtcggctat cagaacaccg gctcctgcat ttcctccatt acgtatatcg acggagacaa
    20461 gggaattttg cgataccgcg gaattcccat cgaggagctc gccgaaaaag caagctttac
    20521 cgaggtggcc tacctgctca tctacgggga gcttcccaac cgggagcagc agcagctttt
    20581 ttcccagcgg ctgacgcaga actccatgat tcacgaggaa atgctgcggt tcttcgacgg
    20641 gtttccgccg accgcccatc cgatggcgat tctggccacg atggtaaatt ccatgtccgt
    20701 gtactacacg gattatttca cggaagattt ccgggccgag acgtttgatc tgatggccgc
    20761 gtcgctgatt tcccgcatcc gcaccatcgc ggcgtactcc tacaagcaca gcatcggcca
    20821 cccgcatgtc tatcccaaac gggacctgaa atacgcgtcc aattttctct acatgatgtt
    20881 cgattctccg gtgaatccct atgtgccgga tccggacatc gagcgggctc tttccacgct
    20941 gatgattttg cacgccgacc atgagcagaa ctgcagcacg tccgccgtcc gtctggtggg
    21001 cagcagcatg gccaatctgt acgcgtcgat ctgctccggc atttgcgcgc tctggggtcc
    21061 gttgcacgga ggcgccaacc agaaggtgat cgagatgctg gaagagatca aaggcagcaa
    21121 gctgtcgatt caaagctgca tcgattcggc caaagacaaa acgagcaagt tccggctttt
    21181 cggcttcggc caccgggtgt ataaaaacta cgatccccgc gcccggattc tgaagcagta
    21241 ctgcgaaacc attttcaaga agcacaatta tcaggacccg ctgatcaata tcgccatgga
    21301 gctggaagac gcggccctga aggatgattt tttcattcag cgcaagctgt atcccaatgt
    21361 cgatttttac agcggcatcc tgtaccggat gatcggcatt cccaccaata tgttcacggt
    21421 catgtttgcc atcggccgtt tgccgggcat gatcgcgcag tggaaagaaa tgcatgatac
    21481 ggtgcccttg aagatcggac gtccgcggca gatttatacc ggcagcaacc tgcggcacta
    21541 cattccgatg gatgagcggg gatagcgggg gatccgataa aaaaaccggt ggaaaatatt
    21601 tttcttgact tttgcccgct gtttgcctat atgccgcgca agggaatgat gcaaatgatg
    21661 aaaacacagt tcaacgttca gacagtcggc tttttcttct ttagctttat cggcgacagg
    21721 atctgcctgg acgtatcatg acctgacatc aagggaaatg aaccgtgggc ggatgaaaat
    21781 tcgcccacgg ttttttttta tttaaaaatc gcaaaaccaa aacaggggga ggggaccatg
    21841 aagacaatcg gcaggatttt acttgtgatg ggcatcttgg cgatgacggc cttgacgggg
    21901 caggccgtcg cccagaccaa agccatcaaa gtcggggcgg ccatcaatct cacggggccg
    21961 gcatccagtt gggggcagtt tcatgaaaaa gggcagcggg actacttccg ctacgtcaat
    22021 gaggaaaaag gcggcgtggg cggccgcaaa attgaaatga tcacgatgga caccgcctac
    22081 aaggtgccgg aggcgcaggc cgccgttcaa aaattcattc tgcaggacaa agtggacatg
    22141 atcgccacgt ggggcgccgg cgaaggtctg gccgcgaaac ccatcgtcca gaagcacaaa
    22201 gtgccgacga tcaattactc caccagttgg gaaatcctgg aaaagccggt gggctacatg
    22261 tatctgccgt tcggaagtta caagatggat tgccgggcgg tccttgaata catcaaaacc
    22321 attcacacgg gcaatggggc gccgaaagtc ggtttgttga cctacaacaa tgcctacgga
    22381 cgatcgattc acgcacccag cagggaatac gcggcaaagc tgggcgttca actggtggcg
    22441 atcgaggaat tcccgcccag aaccgtggac ctcaacacgg agcttctccg cctgcagaaa
    22501 aaaggcgccc agtacatttt cattcagatg ctgccctcca ccatcatcac cgccttcaaa
    22561 agcgcggacc gcatcaaata caatccgctg tttctgggaa cctggacatc caccgatccg
    22621 gacttcttca ggatgggcag agggctgatt cgcgaccgcc tggtcatgca gttccccggc
    22681 ggattgccct cggacaaagc caaggggatg gaggtgatga aggaattgtg gaagcgctac
    22741 aagacggtca aaagttttga tgcgtcctac tgggagggcg ttgtggtcgg catgatcatg
    22801 gaaagggcgt ttctgcgggc ctgggaaaaa tcaaaggtga tcaatcccga gaccgtcaac
    22861 gccgcgatgg aatccatgaa aaatgaagat ttcggcggac tggttccggc ggtcagctac
    22921 tcgaaaacga accatgaggg ctccttcacc gcccggatgg tcaaagtgat ggaaaacgga
    22981 acgtatattc ccttgacaaa tttctacatt ccgggaaaag acaagatcaa agttctgaag
    23041 cgatagggca aaaggcgcgc ggggaccgga tcatgcggtc ttcccgcgcc aatgaagcgc
    23101 ggcgggccag cccgtccaga ggcgtttatg aaagcattcc gcagccggcc ttcgatcatg
    23161 atcaaccggc cccaggaaga taaaaaagcc gaactggaga ttcgcaatct gcggctgagt
    23221 ttcggcggcg tggtggcggt caaagacgtg gatctgtccg tccacaccgg cgaactcacg
    23281 gcggtgatcg gtcccaacgg cgcggggaag accagcctgc tgaactgcat taccggcttt
    23341 taccgccctc agcagggcag catcgttttc aacggccggg atattacgca cctgcacacg
    23401 caccagctcg tcggcatcgg catcggccga acgtttcaga acatcgagct tttcccgggc
    23461 atgacggtgc tcgccaacct gacgctggcc cgccacatcc actgccgcta cagctttctg
    23521 cattcgtgcc tctttacgcc gtccgtccgc gaagaagaaa tccgccatcg ccggattctc
    23581 gaagaaatca tcgactttct ggaaatgcag tccattcgca agcaaaccgt cggctccctg
    23641 ccgtacggaa tgatgaagcg cgtggaattg gggagggcgc tggcgctgga gccgcggctt
    23701 ctgattctgg atgaaccgtt cgcgggaatg aatctggaag aaaaagagga catggtgcgc
    23761 tttctcctgg agctgaacgg acgatggggc ctcacgatgg tgctggtgga gcatgacatg
    23821 tccatcgtca tgagcatctc ccggcacgtc atggtgctga actttggcga gaagctgggg
    23881 gagggtgctc ccgcggacat cagcgccaat ccggaagtga ttaaagcgta tctcggcgat
    23941 gcgcagtcgc tggaataaag aggacgaagc gctttggaca aggcaacccc gcacccaccc
    24001 aaaccaaacc agatcacgct gccgcagctt ctggccgcaa acgccgggaa atacggcgac
    24061 accagaacgg ccatccgcga aaaagccttc ggcatctgga agaaatacac ctggggtgaa
    24121 tatttctcct tcgtcaaaag aacggcggcg ggcttcgcct ctctgaacct ggcgcgggga
    24181 gacaatctgt gcatgatcgt caacaattcg ccggagtggc tcttttccga gctggccgcg
    24241 catgcgctgg gcgcgacgtc gctcaatctc ttcacctctt ccatcgccga ggaactggcc
    24301 ttctccatcg ggcgcgtccg ctcccccctg gtcgtcgtgc aggaccagga gcaggtggac
    24361 aagcttctgg aagtcaaaga caagctgacg caggtgcgat tcgtcgtgta cgttgatccc
    24421 accggcatga cgtcgtatga agacaacccc tggctgatca gcttcgcggc gcttctggaa
    24481 cggggtgaaa ggcacatggc cgatcaaccg gattatgtcg atgcggaaat cggcaaaggg
    24541 aggccccggg acatcgccgt catgatgcag acctccggca ccaccggcgt gccgaaaatc
    24601 gcgatgctct cgcaccacaa cctggccgcc atggcgcatc agtgggtcga agccgcgcac
    24661 attcggccgg aagaaaactg gatctccatg tcgccgacgg cctggatcgt cgaccagatg
    24721 tggggcatgg gcgtggcgct cgcgggcgcg atgacgatga actttccgga aacgccggag
    24781 acggttctgg aggatttccg ggatatcggt ccgtccctgc tcattacggc ctcccgcttc
    24841 tgggaggaca tggcttcccg catccgcgtg aaaatcaccg acgcgggctg gctcaaaagg
    24901 aagctctttt acctggggga aagcgtcggc agagaggtcg tcggaaaaaa attgcgcaga
    24961 gaaagcattt cctggccgct gggcctcttg taccgattcc tgacgctcgc ggtttacaat
    25021 cctttgctgg accgtctggg ctgttcccgt tttcacagcg cctttaccgg cggccacccg
    25081 atcagccccg acgtcatcgg gtttttccgg gcgatcggcc tgaatctgaa gcagtgttac
    25141 gggctgacgg aatcctgcgg gatttttcag attcagccgg acgacgaggt caagctggaa
    25201 accgtcggca agccgcttcc gaacacaagc atcaaactgg ccgaggatca ggaagtgctg
    25261 gtcaagtcgg aggccaattt ttccggatac tacaacgact accagtccac ggaggcggcg
    25321 tttgaaaacg gctggctgaa aaccggagac gcgggctaca tcgacgagga cggccacctg
    25381 ctgattatcg gccgcaagga agacatcatc cgcaacaaga gcggcgacgc cttctcgccg
    25441 gacttcatcg aaacaaggct caagttcagc ccctatatca aggaagcggt cacgttcggc
    25501 gaggcgaggc ccttcatcac cgccatgatc aacattgatc tgggcaatgt cggcagctgg
    25561 gcggaagaaa ggatgatacc ctacacgacc tacatggatc tctcccagca gaaagccgtg
    25621 gaggaaatga ttttacggga agtccgcgag gtgaacggcc agcttcccga ggtgatgaag
    25681 atcaaaaagt tcgtcctgct ctacaaactg ctggacgcgg acgacgacga actgacgcgc
    25741 accggaaaag tgcgcaggcg cttcgtttac ggcctgtaca tcaagatcat tgaagcgatg
    25801 tatgcaggag cgcaaaacgt gccggtgacg gggaaaatcc gctaccgcga cggcagaatc
    25861 ggcgagatcg aaaccatcat caacatcatc agcgtggatt gaggaaccgg tttatggaat
    25921 tttttctgca attgctggtc aacggggtct gcatcggact gctctacggc atttccgcca
    25981 tgggcttcgt gatgattttc aaagcgtcca gcgtgctcaa ctttgcccac ggcgaactgc
    26041 tggcgctggg ggccttcttt ttcctgtccc tggtcacctg gggaaaaatt ccggtcgcgg
    26101 cggcgtttct tttgacgctg atcggttgtt ttgtcctggg cgtcctgatt gaaaaattct
    26161 ttttgcgtcc gctcatcggc gaaaaactga ttttcgtcat tatgctgacg gtagggctgg
    26221 ccgccatgtt caagggcctg attctgcttg tctggggcgg caacctgcac acctatccgg
    26281 agttccttcc cgcgtcgctg gagtttcatc tgggctcaat ctacatcgcg cccgtgtatt
    26341 ccgccacgct ggtcatcagc gtcgtatttc tggttctctt cggcctgttt tttaaaaagt
    26401 cgtcacaggg catcttcatg cgttcggtgg cggacaatca gaaagctgcc ctttcgctgg
    26461 gcgttcacgt ccgccgcgtc ttcgcgcttt cctgggcgat cgccgcgatg gtggcctgca
    26521 tgagcggcat cgttctgggc atcatcaacg gcgtaaacgt ccacgacttg tccgccatcg
    26581 gcctgaaagt gtttcccgtg gttattctgg gcggtctgga cagcatcggc ggggcgatca
    26641 tcggcggaat catcatcggc cttctggaaa ccttcacggg cggctatctg tccccgtcgc
    26701 tgagagacgt ggttccctat atcgtgctgg tgttcatcct gatggtgaag ccctacggcc
    26761 tgttcggcct ggtggagatt gaaagagtgt aatatgaaga agccgtcttt tcatccctgc
    26821 gggaatttcc gcgaaaccta cgccgaggag ctggcgattt tcgacacctg gttcggccgg
    26881 gtcagcctca ttgtcttcct gcttctgctg ttttgcctcg cgccgttgat gctggaccgt
    26941 tatctgctgt acattctgaa cagcatcggg atcatggcga ttgccgccat cggcctcaac
    27001 attctcatcg gctacaccgg acagatctcg ctggggcatg gcgcgttttt cggcgtgggc
    27061 gcctacgcgg gagcaattct ggccacgcgc ctgaatctgc cgttcttcgt ctgcattccc
    27121 gccgccgggc tgatcaccgc catggtcggc atgattttcg gaattccttc gggccgcctc
    27181 aaggggctgt acctgacgat cgccacgctg gccggccagt tcatcatcga atatgtcctg
    27241 atccagtggg aaagcctgac caaggggacg atggggatca cgctgccgga gccgcaggtt
    27301 ctgggcgtca acatttccgg agacaaggcc tttttctttc tgattttcgc ctgcctggtg
    27361 tgcatgacct ggtttgcggt caacatcatg cgcagcaaat acgggcgcgc gtttgtcgcc
    27421 attcgggaca acgaccgcgc ggcggagggc atgggcattc ctattttccg ctacaagctt
    27481 ctgtcgtttg cgatcagttc gttttacgcg ggcttcgccg gctccctctg ggcctactac
    27541 atggtcagca tcacgacgga gcccttcaat ctcgcgctgt ccgtggaatt catcgccatg
    27601 gtcatcatcg gcggcatggg caacattccc ggcgcgattt tcggcgcaac cttcatcacg
    27661 gtgctcaacg aactgctgcg cttcgcgacg ggcgccctga tgaacgtcgg ctggattgcc
    27721 ggactggggc tgaacatggc gccgcttcga gaatttgttt tcggactggc gattgtgctg
    27781 tttattttac tggagccgaa ggggctggcg gagctgtggc gcatcgtgcg ctccagcttc
    27841 cggctctggc cgttttcgta ttagtaagtt agaaattatt ttctgagttt tttgcagaaa
    27901 ataatttcgt taacgcactt atcccgcata acggggtcag gttgatggta tgggcgaaat
    27961 tcttctgcag ttgaacaata tttccgtcgt ttattccgac gtgattcagg tgctcaaagg
    28021 cgtgtcgctt tcggttgcta acgggcatat cgtatcgctt ctcggcagca acggcgcggg
    28081 taagaccaca accctcaagg cgatttccgg ccttttgaag cctgaaaacg gggaggttac
    28141 cgacggcagc atcatcttca acggtgtgaa tattcagaat caggcgccgg agtttattac
    28201 ccggcagggc atcattcagg tgctggaagg ccgccagcct tttaaatatc tgacgattga
    28261 agaaaatctg cgcgtcggaa cggccacccg gccgggcaag ccctacaagg aagacctgga
    28321 aatggtttat tcctactttc cggcgctggt gaaaagaaag aaggcgctgg ccggctactg
    28381 cagcggcggc gaactgcaga tgctggtcat cggccgggcg ctgatggcgc atccgcagct
    28441 tttgatgctg gacgagcctt cgctggggct cgcgccgctg gtagtgcagg aaattttccg
    28501 catcattcga aagatcaatg aagagcacgg cacaacgatt gtgctggtgg agcagaacgc
    28561 ccatatggcg ctgcaggtag cgcattacgg ctacgtcatg gaaaacggca aaatcgtcat
    28621 ggagggcacg gcccgagagc ttgccgacaa cccggacgtc aaggaatttt acctgggaat
    28681 ggccgcttcc ggcatcgcca aatcctacaa agacgtcaaa tcctaccgcc gccgcaaacg
    28741 ctggctgtaa aaggagaagg gactatgaag cggttatacg atgaaaaaga agcgtggagt
    28801 ccggaaaaga gaaaagcctg gcaggatcgg agattgtcgc gcatggtgag gctgggctat
    28861 cagaagtcac ctgcgcttcg ccgaatgctg gacgcgaaca aaatcaatcc cgcccggata
    28921 aaaacccggg aagacctgga agcgctgccc gtcacctcgc gggaaaagct ggtggagatg
    28981 gaaatgcgca agccgcccta cgcgggcctg gaaaattcaa ccatcgccgt cgaccgtatt
    29041 ttcacttctc ccgggccggt ttacgaaccc catttatcgg aaaacgattt tctctgggcg
    29101 cgcgctttct ttgcggccgg catcggttcc agggacatcg cgctcaatgc gttttcctat
    29161 catctggtgg cggcgggcct cacgtttcac aacggcctgc ggaaagtcgg cgccacggtg
    29221 gttccttccg gcacgtcctc ctcgcagatt caggttcagc tcatccgcga cctgaaagtg
    29281 accgcctacg tgggcacgcc aagcttcctt atgtccatta tcgatcagtc caaagagatg
    29341 ggctatgatt tcaaaaagga ttttcgcgta aagaaggcct gcttcgccgc cgagccgctc
    29401 ctgccttcgc tgcggcagag atttgaaaag gaatacggaa tcagaacctt tcagatgtac
    29461 ggcgccaccg aagtgggcga cgtggcctac gaatgccccg ccaaatccgg ctggcatatc
    29521 tgtgaggaaa cgattgtgga aatcgtcgat ccggcaacgg gaaagaatgt cgcgccgggc
    29581 gaactgggcg aggtggtcgt aacgcgcctc aatgatcttt tctttctgct gcgcttcgga
    29641 acgggcgatt tatcccggct cgtcaccaaa aaatgctcct gcggaagaac ctctttccga
    29701 ctggcgggaa tcgcgggcag ggtaggcgat gccgtaaagg tccgggggct gttcattgct
    29761 cccagccaga tgaagctcct ttccgccaaa ttcgaaggcc ttcccctgca ggccgtggtc
    29821 agccgcaaag gccatgaaga cgttttgacg ctgcgcatcg agaaaataaa ttctcaagta
    29881 aataccgaca gctggaaaaa taacttcaaa aaaatattta aggaattgtg ctcggtccga
    29941 atcgacgttc tggaggttgt ggaaaaaggc ggaatcaaaa cggatcagaa ggtgatcgtc
    30001 gattcccgga cgtggtaggg ggctctgtct ttttgccgag gcgatacgcg tcaatcgtcg
    30061 aggagatcca caaaaacaaa atcaaaatca gcagacccag ggcaaggttc aaatcgattg
    30121 tccggagtgc attctccgcg tttcggagaa tgcgggtcgt gcctgcgcct tccgatgccg
    30181 catcccggac gatcatgaga acaacctgaa aaatcatcca ggccagggta agcgttgcgg
    30241 ccgccgccgg aatcagaaaa gccattcctc tcgcatatcg cttcagatag atctggcctc
    30301 cgcccggaat gaggagaaag ctgaacaggg cggccctggt cgcatcattc atggcgactc
    30361 ctcaggcgac gatggccgcg tccctgattt tcaattgaac gtcggatgag ccgttccagt
    30421 agttgatctg aggagagaag accacgtcga agctggcctg gttgatcacg cctagatatt
    30481 tgcccatgcc gaaccagatg gcgctgtggg acgcgccgtc gtcaaccacg cgcattttga
    30541 gatggttgtt gccgacaatg acgggagatg aagccttgat gtttctcgag cagaggaccg
    30601 gttcgggatt ctcgcagccg aaaggcgcga gcattgacat ctgccggatc agatcgaggc
    30661 tgatgtcctg caggcggcat tcggcgtcga tcagggtctg cgaaaccatt tccgaggact
    30721 gcaccgtgtt gcggatgatt tcatccagca ggcaggcgaa ttcgtcgatg tcgtcttcct
    30781 tgatggaaat gccggccgcc cggtagtggc cgccgtagga cagaagcagc ggcgcgcact
    30841 gctgcaggcc cttgtggatg ttgaaatcgg agacgctgcg gccggagcct ttgcccacgc
    30901 cgtccttcag gctgatgacg aaggtcggac ggctgaagag atccaccaga cgcgatgcca
    30961 cgatgcccac gacgccggga tgccactttt cggaggaaaa gaccagggcg ctgatgtttt
    31021 ccaggtcggg tttggcgccg agcttttcca ggatgtcgtt caggatgtct ttttccatgg
    31081 cctggcgccg gcggttgtgc aaatccagct tctccgcgag cggccgtgcc tcctccatcg
    31141 tttccgccag cagcagttcg acggcatcga gcggcgaggc gatccttccc gccgcgttga
    31201 tccggggaat caggcagaag gaagccttga aggaatcaac gagctgtccg tcgatgccgc
    31261 tgacttcctt aagcgctttg attccggggc gtctgccttc cgtgatgaga tccaggccga
    31321 ttctggcgaa gattcggttt tcatccagaa gcggcgcgat gtcgccgatg gttcccagag
    31381 caacgacgtc gagaaattct ttcaggttgg gataatcgtc gtttttccaa aagccttccc
    31441 tgcgcagcgt tccgcgaagc gcgatcagga aattgaaggc gatgcccacg ccggccagat
    31501 gtttgaaggg aaaggaacag tcggaacggt tgggattgat gcaggcgacg gcaggaggaa
    31561 gctgatcggc gatttcatgg tggtccagga cgatcgtatc gatgcccacg gatttggcgt
    31621 aggcaatctg ctcgatatcg gagatgccgc aatccactgt aacaatcagc ttcacattct
    31681 tttgtttgaa ccggtcgatg accggaatct tcagtccata gccttcctgg acacggtccg
    31741 gaatgtaata ggagacgcgg gcggtgagct gtttcataaa tttgtacaaa atgacgacgg
    31801 aggtgattcc gtccgcgtcg tagtcgccgt aaatgacgat ctcttcgccg tcgtaaatcg
    31861 ccttcagcag cctggccacc ccttttttca tatcctgcat cagaaacggg ctgtggaggt
    31921 cgttgaggga aggatgcaga tagcggtgcg cgtcttccaa gctcccgacg cgccgtttca
    31981 ggatcatctg ggaaataatc gggtggaagc cgaattctct gaccagcgat tcttctatcg
    32041 ttttagggcc ggaatccttg attttccatt gcgttaccgg cagttgtccc cgttcattca
    32101 tgtgttcttc tttaacgttt tatttttcat cgcaatcata tcagtgatcg ttcatatgtc
    32161 aagtcttcat tggtcttggc aggcgttctt gatctttaaa acaataaatg ctaatcgttt
    32221 tgctgtaaat ttcgagacaa tagggcaccc ccatgctgat gttaacccat acctgtttat
    32281 tgcagcaaat gatgagcgcc tccggaattc aaaatatgga tccggatatt tacatttata
    32341 atatcgcccc ggatttattg acgattcatc ccgatatcaa cccccggcag actcattcca
    32401 ttccgcgttt catcgaggcg ccgcccgacc atctgcgaac ggcctacatc atgtttcatc
    32461 tgctggttga tgatctggcg cattacggca gcatctcgat gacctgcaag gaaggtttcg
    32521 atccggactc ctcgggctat acctatctca aggggcggaa tcttgtccgc tccatcatgg
    32581 agctgcatgc catcatcggc aaggacatct cctacagcga ggccgcttac cgttctcatt
    32641 taattattga aatgatctat gacctgatca ttgggccgca aatcgacaaa aacggttcca
    32701 tcgaccttct ggaagaggcc attcatttta catttgaccg gagggaaaag gaattttgct
    32761 ctgatatttc atggctttac ggcatcgatt tcgaacaaag ccgggaggtg ctgaaaattg
    32821 cggtgtcgta cattaccggg gaaaggctga gaaggatcat gaatatcgaa ggccggattc
    32881 gcctttttac ggacaagttc ggccttcgga acaacaatga gatgtttgcc gaagccattg
    32941 cagctctgtt tcaggacgcc ctggattcca ttgaaaacga cgactttctt caggaaaccg
    33001 cttcgaccat tcgaagctgc ggctggcttc cgacgcgtta gcttgctttt ttcacaaaac
    33061 cggagcggat gcagcgcgtg cagactttta cccgcttcac ggctcccgtt ttttcatcca
    33121 cgcagcgcaa tttctgcaaa ttcggacgcc agatcacgcg ggtcttgttg ttggcgtggc
    33181 tgacgttgtt gccgatggcc ggtttctttc cgcatacttc acaaatccga gacatgacga
    33241 gactcctttt aaaaaagtgc gtggttacta agccagaatg gtttattttg caagcatttt
    33301 tttcttcctg accaccttca gccttcggcc ttcagtctat tcacctgatt attcctcacc
    33361 gccttcagcc ttcggccttc agtcttcagt ctatttacct aatttttcat ctgtgcgggc
    33421 tgcccggttg attccagcac acgaaaccag agatttccgt ccgggtcgat ctgcctgccg
    33481 gatatcgccg cggaaagggg cagatgaaca tattcatccc tgcgcagcgc caccagcagg
    33541 ccggttttcc ccgccatggc ggcgtgcacg gaatactgcc ccagagagct gcagtaaagg
    33601 ctgtccgaag cgttggccgg gacggagcgg atcatatagc tgggttcaat atatttcaga
    33661 ttgatttcca catgcgcttc gcggaaatgc gtttcaatgg cacttttcaa aaacaggccg
    33721 atgtcctgca gacggacatt gccggaggca tccgtttctc ttttcttttc atcctgctgc
    33781 atcaattcct gcccggcgcc ttccgccacc agaatcacgg cgtgtccgct tgccttcatt
    33841 cggttttcca gcgctttcag aaaccccttt tctccctcca ggtcaaacgg cacttcggga
    33901 atcagaacaa agttggcgtc tccctgggcc agcgcggtcg tggccgtgat gtagccggac
    33961 aggcgtccca tgattttcac caggccgatg ccgttgtagg cgcccttggc ctcgacatgc
    34021 gcgcttccga ccgcttcgac ggttttggcg atggcggtgt cgaatccgaa ggatttctca
    34081 atcaggttca aatcgttgtc gatggttttg gggatgccga taacggcgat gggatctttc
    34141 cgggcatgga tctcctccgt gattttttcc gcggcgcgca tggtgccgtc gccgcccacg
    34201 caaaaaagaa tgtcgatttt caaaagctcc aggtagtcca ccatcacctt cacatcctga
    34261 ggaccgcggg acgaggacag gatgctgccg ccgatatgcg tgatgtcctt gacgaagtcg
    34321 ggtgtgagct ggatgggtat tttgccgtat tccgggttca gtccgcgcag tccgtatttg
    34381 attcccagaa cgttttttac gccgtaacgg taaaacagcg tcatcgtcac cgagcggatc
    34441 acgtcattga tgcccgggca gaggccgccg cacgtgacga tggccgcttt cgtcccggcg
    34501 ggatcaaaaa atatttttct tcgggggccc gccggctcca gagacaaagg ctttccggtt
    34561 gaaaccatca gcgcgtcgca atctttcagg cgggtgttga acaggatccg gtcatcgtcc
    34621 gaaacaaatc cgttgatggc aagcggcgaa tcgatggcgc agacgcccag aacggaaatg
    34681 gcgaagtcgt cttgcatacg attatttccc tttctgcagg accctcgaaa cggcgtttct
    34741 gccggaaagg atctccgctt caaaaccggc gtccgcgagc agcgacgtcc cggtcaaaaa
    34801 gacattgctc catctcgtcc ggtcgttctt ggccgaaaaa gacgtaaaaa aagcgttgcg
    34861 caccctgtat ttcggagaga atactttacg gtacttcaac gaaagttcga tggacttgtc
    34921 aaggtcgtac atctcgatgc cgtctttcaa gaaagggaaa aactgatcga gcctttccag
    34981 gatggattcg gcttcccgcc gcagggcctc cggagcccag gcggcatcat cggccggcag
    35041 gtaaaccgtg gcggtcatga aattctttgc ctgcggcagg ggttttcctt tttcgggcat
    35101 gccggtttcc agaagaatca ggtttcggtc gtgaagatcc ctatggacgt ccgcgacgac
    35161 ggccatgtga cgggccatct gttcgggcag gaacttctgg gcaacgccca gaaaaagggt
    35221 aaatggatag ccggcaatgc ccgccttgcg gaacttgtcc caaaaaaagg cgtggccgcg
    35281 tttttgcgtg atgaggggca atgcctccga tttcgtgctg acgatcaaat aacggccggc
    35341 aacccgggat gtggatttgt ctggagcttc catctccagc gtgattgttt tgccccggtc
    35401 gagggacttg acggcatgcc cgctcaggta cagaccgccc tgggattcca gcgtccggat
    35461 catttcgttg aacaaaaact gcttcccctg cggaaaacag gcgacgcctc gcagcggcgc
    35521 gctgtattga aaagcggagg caaaggaaaa caaatcgtcc gtattttgcg aaagcagcgc
    35581 ctgctgcgct tcccatactt tttccagcgc cgcgtcccgg gagagcattt tgtcaaacct
    35641 ggcggcggca aatttgtact gcagcacgaa gggatagatt ttcagatagg aaaagtattc
    35701 cttcagggtt tgcggctgca cccggggatg ttcggcaatc cagtcctgaa aaacggaaga
    35761 tgcttgaagc gcaatgccgt aaaaatcgcg gatgtccgtt tcccgcttcg gaaattcccg
    35821 ggccagttcg gaaaggagcg cttcggagtc gtttgagaaa tcgatccggt ggcccgggag
    35881 cagcacctgg tacgcggggc tcatggacgt cggatgagtt tcgggaattt caacgcccag
    35941 ttcggcaagc acggaaaagc cgggctgacc gggagcgagg ccggtcatcg gcgcgggatc
    36001 aagatggaaa acaaagtcgc cgatgagctg cagcccgccc aggccgccgg gggcaaccag
    36061 gagcgtcttg agtccgtttc ggctcgcgca ggcggccgcc acgtgggaag cgagatcatc
    36121 gcccagcacc agcaaatcgt acacgaatga catttcctcc aaaacaggcc ggcggaaagc
    36181 ggcgttcagc cctccggggg cggattgctg agcgcggcgt catggtttcc ggcgtccctg
    36241 atctctacgc ggcgaccgtt tctgacaatt cggccctggg cgaaataacg gatggcctgg
    36301 gggtaaattt tgtgctcttc cttcaaaatc cgcgccgcga gctcctgggc cgtgtcgccg
    36361 gcaaaaacgg gcacaaccgc ctgaatgatg atcggacccg tgtccacgcc ttcatcgaca
    36421 aaatgaactg tgcacccgga aaaacgaacg ccgtaatcca gcgctttctg ctgaacgtgc
    36481 gtgccgggga aagaggggag cagggcggga tgaatattga tgatccggtc cggaaaggca
    36541 cgaagaaaaa acggcgtaag aatccgcatg aaaccggcga gcacgaccag ctcgacgccg
    36601 gcgccctgca ggatgcgaag cagttcgcgg tcaaacgctt cccggttttc aaaatcccgg
    36661 tggcttacca ccgcggtggg aatccggtat tgttgcgcac gggtcaggcc gtaggcgtcg
    36721 gggcggttgc tggccaccat agtgatccgg gcgccaagcg cccccttttc gatgtgatcg
    36781 atgatggact gaaggttgga gccgcttccg gaaatcagga cgcccagttt taaaaggtca
    36841 gccataaaac cgccttacag atccgtaaaa cagaccggtg gcccgttctc gtcccgcttt
    36901 tcgatcgttc ccaggagaaa ggcgttttct cccagcccct tcaggcggtc gagaatttcc
    36961 tgcacatctt tttccgggac gatcatcatc atgccgattc ccatgttgaa cacgcggaac
    37021 atttcacgct catcgacccg gccgatttcc tgaatgacgg aaaagatcgg cggaatcggc
    37081 cagctttcct tccggatcac gcagcggcag acatcgggaa tgacacgcgg aatgttgtcg
    37141 tagaaaccgc cgccggtaat gtggacaagc cccttgacct gaaagctttt gaagacattc
    37201 aggagaggct tgacatagat gcgggtcggt tcaagcagct cctcgccgac gcttcgtcca
    37261 agctccgcaa cgctgtcctt cacggaaagt ccgccttttt ccagaagcac gcgacgggcc
    37321 agcgaataac cgttgctgtg caggccgctg gaggccagac cgatcacgcg gtcgttgatg
    37381 ctgatggtag agccgtcaat aagcttttcc gattccacca cccccacgca gaatccggcc
    37441 agatcatatt cacccggcgg atagaatccc ggcatttcgg ccgtctctcc acccaagagc
    37501 gcgcagccgg cctgtttgca gccctggacg atgccttcca cgatctgaac gcttttttcc
    37561 gtttcgattt tgccggtggc caggtaatcc agaaagaaca aaggctccgc tccctgaacg
    37621 accacgtcgt ttaccgacat ggccaccagg tcgatgccga tggtgttgtg ccggtccgtc
    37681 aattgcgcga ttttcagctt ggttccgacg ccgtcggtgg aggaaaccaa aatggggttt
    37741 ctggccttgg cggtgtcaaa acggtacaaa ccgccgaagc ctccgatgcc ggacaggact
    37801 tccctgcggg ccgtcgtttt gatcaggggc ttgatgcgct cgacgaaggc attggccgtc
    37861 tcgatatcga cgccggattc tttatacgtc gaagaggttg attttatgga tttagttggc
    37921 atagggctcc atcaggacag gttgtatttt ttacacttgg cttaagacga aaacgattta
    37981 acgtaaatcc ccttgcgtgt caatcctcga cttgcgcaaa caaaatttat ttgttaaaaa
    38041 acaaacaaat tcataaacgc attttccgca ccttccgtat ccggagcatg atgaaatgga
    38101 gtcttttaaa aagaatcttc agcgcatcga gcagcctctg aattttgccg ctaaagatca
    38161 atttaaaaat ctcccgaaca tccggaacct ggggaaatcc ctctcgggct tgatcgcttt
    38221 gcagatatcg gccattccgc ccgccgcgaa aaacattttc ggccccgccc tggaccacat
    38281 gatgcgaatt tttcaggaat acgacggcaa ggacctccag cagaagaaaa gcggcattgc
    38341 cgaagcctcg ctcatcctgg aacggctcaa gtacgccgcc gacaactttg acgcgcgcaa
    38401 tccgggcaat ctgcaggaag agcagattgc cgcccgcgtg gaagacctga aaatctccgc
    38461 ggaaaagctg tccgcgccgg tgcagtttct gaagggcgtc gggccgaaaa tggctgaacg
    38521 gttttctgca aaaaaaatcc agaccgtcga agatttgctt tattttctgc cccgaaccta
    38581 tgaagatcgc cgggaaatcc ggaaaattca caagctggag acgaacagga tccagacgat
    38641 catggccgcc gttgcggact gctctttccg ctgttacggg aaaagaagga ttctggaagt
    38701 cgccgtgagc gacaacaccg gggtcctcac ggccaagtgg ttcaagggcc ggatgtctca
    38761 tctggccggc gcttttcaaa agggaacccg cgtgattttc accggcaacg tgacgcccgg
    38821 ctacgcgggc aagtccatga ttcatccgga ttacgaaatt ctggacaaag aggacgaaga
    38881 aaatctgctc aatttcaggc gcattgttcc ggtctattcg gaaacggagg gcctccacca
    38941 gaaatacatc cggaaagtca tggccttggc gctggaaaat tattcgcgct acatcgcatc
    39001 gccgattccc gcggacatct gccggaagag aggcctgatc aacatccgcg aggcgcttgt
    39061 gggcgcgcat ttccccggga gcgacgcgct catggaacct ctgcaaaacg cgcgctccga
    39121 ggcgcaccgc cgcctgattt acgacgagtt tttctttttt cagctgggaa tggccgtcaa
    39181 gaaatccgga cgcgttctgg atcgcggcat ttcctttgcc gccggcggga gccgactggc
    39241 aaaattttac gcctcgctgc cgtttgagct gaccggcgcg caaaagcggg tggtcgatga
    39301 aattcacaaa gacctgcatg cgccgacggc catgaaccgt cttctgcagg gcgacgtggg
    39361 aagcgggaaa acgctggtgg ccatgtcggc catggtgacc gtatgcgaaa acgggtatca
    39421 ggccgcgctg atggcgccca cggaaattct ggcccggcag cacgaggcga ccctgtccgc
    39481 gtgggccggt ccggtcgggc tgcgcgtggt cctgctgacc ggcggcatga acggcgcggc
    39541 tcgcacagag gcgctggagc gcatcgggtc gggcgaggcc gacatcatca tcggaacgca
    39601 cgccctgatc caggagaatg tggatttcag gaaactgggg ctggtcgtca ttgacgagca
    39661 gcaccgcttc ggcgttatgc agcgggtagc gctgcgcaac aaaggccttt ccgccgacgt
    39721 gctggtcatg accgccacgc cgatcccgcg aacgctggcg atgacggttt acggagacct
    39781 ggacgtgtcg gtcatcgacg aaatgccgcc gggcaaaaaa ccggtgcgca cgctggtgat
    39841 gggggaaaac agaagagaag cggtgtacga ggccattcgc aaagagctgg ccggaaatca
    39901 tcaggcgttc atcgtctatc ccctggtcga gcagtcggag aacctggatt tgaaagacgc
    39961 gacgaaaatg gccgaacatc tgcagaaaga tattttcccg aatgtccggg tcggcctgat
    40021 ccacggcaaa atgaaggacc gtgagaaaga cgcggtgatg cgggattttc aggaaaatca
    40081 aatcgcaatc ctggtatcca caaccgtgat cgaagtggga atcgacgttc ctcgcgcctc
    40141 gctgatggta attgagcacg cggagcgatt cggcctgtcg cagcttcacc agcttcgcgg
    40201 ccgcgtcggc cgccgcgata ttccctccac ctgcattctt cttgccgact acaaggcatc
    40261 gggtgatgcc cgcaagcggc tgaaaatcat ggaaaacaca accgatggat ttgtcctggc
    40321 cgaagaagac ctggctattc gcggaccggg ggattttctg ggaaccaggc agtccggatt
    40381 gcctgatttc cgaattgcca gcatcatccg cgacgcgcgc attctcaacg acgcaaagga
    40441 ggacgctttt gcgctgattg tccgcgatcc gcttcttgcc aagcccgaac acgctatttt
    40501 aaaagagacg ctgatcgcga aatggcaggg aaaactggat ctggcccgaa cgggataaac
    40561 aaagggtatg accagagagg attgagaaaa ccggtctgaa gccatcaagg ttaagatttg
    40621 acgaggagga aaacacttag aagtcatcgg taatctatcg gtaaaaattg ggcaatcata
    40681 aggattgacg atagccttcc ggccctgtta atttttgcat aattgaggcg tttagaaaat
    40741 tcgtcatgaa agaaggggga atgactgtct cctcaaaatg ttaaaatgga aaaacagaaa
    40801 ggagtgcctg atgaaaacat caagaatgat cgcattgttc atcgcagttg tttttttagc
    40861 gttttcaaca atggcatttg ccgccggacc gggcaagaaa ccggaccgcg tcccgcccaa
    40921 gcttcagtcc ccgctgctgg gcagtgaaat gctgtgggac cgcccggaag ctttcggccc
    40981 cgtgccaaag tcgaagagaa acctgggcaa gcaggtatgc ggagcggaca gcaaggccat
    41041 cggttatcat ccgaaagccc aggatgagaa aggcaaagtt tttccgaaag gcggttttct
    41101 ctgcgccccg aaataagcgg gttttgaaac ctgaaacgca gaaaactcac ctgtgaggct
    41161 gatgttcaag cctcacagga tctttttgtt ttgaccggac tagccaaagc agcggtaagc
    41221 caagacaatc gatcgaagat gccttccatg aaccccaaaa acatttttta gcggcatttc
    41281 ctgaagattc cataaggcag ttgaccggag ttcagttgtc cgtcaacaac cggaagtcca
    41341 aattgatcct tgatcttttt gaaggatttc ggttaactcg caactgaaaa ggagatccat
    41401 tcgatgagaa tcgcggtggt cggaatcggc ggggtagggg gatatttcgg cggcaaactg
    41461 gcccgggaat acgcagcctc cggaaagcat gaaatcgtct tcatcgcccg cggagagcat
    41521 cttcgggcca ttcagaaaaa cggccttcag ctttgcgccc gggaaggcca atataccgcc
    41581 tggccgaaca tcgccacgga cgatccctcc gtggccggcc ggttcgatct ggtctttttc
    41641 tgcgtcaaaa gctattcgct tgaggaatcg gcacgctccc tggcgaagaa catcggcaaa
    41701 acaaccgtgg tcatccccct gctaaacggc gtggacagcg ccgaaaggct gaaaggggtg
    41761 ctgccgggcg cggacgtcgt cagcggttcc gtttacatca tcagttttct ggaccagccc
    41821 ggggtggtga tccagacgca gggcgcctgc cgattgattt tcggcacgga tgaccgggtg
    41881 tccgccgacc ggtattcccc tgttctggaa ctcctgctgg gggcgaaaat cgacgccagg
    41941 ctatcgggcc gcatgaccga agcgctgtgg atgaaattcc tgatgatgtg ccccctggga
    42001 tcgctcacgg cggcaaccgg caaaacgtac ggtttgatcc gcgatgacgg ggatttgcaa
    42061 aaccggctgc gggcgatgat gctggaagtc gccgcggtcg cccgtgcccg ccgcgtgcac
    42121 ctgccggaca gcgcggtgga caaaaccata gagctgatcg gcggcttctc ccatgacgcg
    42181 aaaacctcca tgcagctcga ccgggagaga ggccggcaga cggaagtgga caccctgacg
    42241 gcgtttcttt gccgggcggg acgtgaagcg gggattccca cgccggttca cgacgaggtc
    42301 tatcgtcagc tgacatcgta gcggacccgg ccccggcgga ggttcatctc ctcatatcca
    42361 tttgacaaaa caacgggatt tgatataggg taccaccctc gcgaaatcag gcggggcaaa
    42421 gcggccccgg gccgtatttt tgatgcacgg ttgaggtaaa aaatgaaaaa catatccatc
    42481 ggcttgatcg gcctgggcaa tatcggaacc ggcgtggtcc ggctcatgaa gcaaaacgaa
    42541 aagctcatcg cccggaagct aggcgcgaag cttgttttaa agaagatcgc cgacatcgac
    42601 atcaattccc cgcgcggcgt gaaaattccg aaaaacctgc tgacgaccga cgcccgggaa
    42661 attctgaacg attcggacat cgccatcgtg atcgaactga tgggcgggta tgagccggcg
    42721 cgcaccttcg ttttggaagc cattcggaac ggcaaacatg tggtgaccgc caacaaggcc
    42781 atgctggcca cctacggcaa cgagattttc ccggcggcgc agaagcaaaa cgtggatatc
    42841 ggatttgaag ccagtgtcgg cgggaccatt cccatcatca aaacgctgaa ggaatcgctg
    42901 gtcgccaacc ggatccaatc catcgtcggc atcatgaacg gaacctgcaa tttcattctc
    42961 accaaaatga cgaacgaggg gaagcccttc gacgtggtcc tgaaggaagc ccaggagctg
    43021 ggttttgccg aggcggaccc gacgttcgac attgagggca tcgatacgtc gcacaagatg
    43081 gccattgtgc tgtcgctcgc ctacgggaag aaagtccgcc tcaaggacat ttacctggaa
    43141 ggcatcacga aaatcagcgc ggaggacatc gcctttgcca gggagctggg ctaccggatc
    43201 aagcttttgg cgatcggcag gctgaaaggc gatgcggtgg aagccagaat ccacccgacc
    43261 atgatcgcgt cgggacacct gctggcgaac gtcaaccaca actacaacgc cttccacctg
    43321 gagggagacg cctccgggcc ggtttttctc ttcgggcagg gagcgggcat gatgccgacg
    43381 gccagcgcgg ttttcagcga tattctggac agcgccc
//
LOCUS       DAOPAF010000009        41909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1072_length_41909_cov_42.347374, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000009 DAOPAF010000000
VERSION     DAOPAF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 41909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41909
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_41909_cov_42.347374"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1143
                     /locus_tag="PLQ77_01975"
     CDS             190..1143
                     /locus_tag="PLQ77_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPL09478.1"
                     /translation="MRLMLFIGLVFFLSCAPAEEPAAKDGRLTVIATIFPLYDFARNV
                     GGDKVAVSMLLPPGSDAHHYELKPRDIVRVSKADIFLFTSFELEQWAYKIINAAAEKT
                     NMLAVETGRGAFLLPLPESPDHDHGAGAAGGIDEAEYASRYDPHIWLDAANAQKMIDN
                     IAAAFITRDPKNSETYRKNAEGYKRRLMDLDEKYREGLSACSTRTLLHAGHWAFAYLA
                     NRYRLHYRSASNSSADAEPSPQQILSLMEQIRKEKLRYIFYEDFTAPKLAAVMADETG
                     VGLLKLNNGHDVGRKDFQSGETYLTLMEKNLAHLREGLRCR"
     gene            1134..1871
                     /locus_tag="PLQ77_01980"
     CDS             1134..1871
                     /locus_tag="PLQ77_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPL09479.1"
                     /translation="MPVIRVEDLTYRYNGAEVLSGIHFTVERGVYLAIVGPNGSGKTT
                     LVKNILGILKPQQGRVDLFGTRIEDFNQWEKIGYLPQRLSGLNTHFPGTVTEVVQMGL
                     PKKDPGLLSRTLDAMAIGHLARRLIGELSYGEQQRAMLARALIRRPQLLIFDEPTTAL
                     DPETRGIFYGLTQELSRKEGATVVLVTHDSGVVGQYAQHLLYLDKKLIFSGTFRDFCA
                     SVDMEGFFGPASQHIICHQHDLKGSGR"
     gene            1871..2701
                     /locus_tag="PLQ77_01985"
     CDS             1871..2701
                     /locus_tag="PLQ77_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPL09480.1"
                     /translation="MDLLDVFTYGFVQRAMLAGSLIAAVSALLGLFLVLRRFSLIGDG
                     LAHTTFGSVAVVLLVGISPFYVTLAALPLVMISSLAIYRLTQSRKIHADAAIGIVSSL
                     GIAAGIILSSLSGGFNVDLFSYLFGNILTVNQTELLLSVIVFALVAAAVVYCYEDLFA
                     VTFDQELARTMGVQTGRINFVLFLLTAVSAVLAMKVAGIMLVSALLILPALTALQLSV
                     SFRKTMMSAVGFAVGAVLLGIVFAFVLNLPAGAMIVLFNIVFLLLVAGVRKFCAGRKA
                     "
     gene            2724..3632
                     /locus_tag="PLQ77_01990"
     CDS             2724..3632
                     /locus_tag="PLQ77_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPL09481.1"
                     /translation="MRVSPFLYMLAIVAGLLLPQAAPWASLLVLPALTIALTVALLRF
                     PGGFFKNPRTLLPGALWGCLMNYLVLGNLMILGGIFLIKNENFWIGLVILAAVPSALA
                     ALPWSEKLGADHRLTLTGFAGTYLGALILIPLLAAAFLRYIPVCCEKLIIMAAALIFL
                     PLLLSRVAVDRNWDERFHQRMGTVSDVCFFIVFYAAASANSVLIRQGSLDMLFILALA
                     FVSTALIAAALIAAGRVFKLSRPVVSSLLLLGTMKNCGLAAGIALYAVNDEAAVPALI
                     FSIFIFLNGLWLWLWRKGLERRFYRA"
     gene            complement(3731..4012)
                     /locus_tag="PLQ77_01995"
     CDS             complement(3731..4012)
                     /locus_tag="PLQ77_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL09482.1"
                     /translation="MNKSGLIEALSKKMNLTEKKAIDVVNLVFKGFNDELKKGGRIEI
                     RGFGSFVVKEYGSYTGRNPKTGTKIKVEPKKLPFFKVGKELKDRVDKNK"
     gene            complement(4022..4846)
                     /locus_tag="PLQ77_02000"
     CDS             complement(4022..4846)
                     /locus_tag="PLQ77_02000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPL09483.1"
                     /translation="MKERLQKIISAAGIDSRRHAEAMITEGRVSVNGEIVRELGSKAD
                     PQKDVIRIDGKIISIENPGCYIALYKPAGYVTTLSDPQKRPTVVDLVRDVPERVYPVG
                     RLDYDSAGLLLLTNDGAFAQKISHPGFRVPKTYRAKIAGRISLEKLKEISKGILLPDG
                     VFKPENLKQEKVNDKSAWISLTLHEGRNRIIRRGFEAAGFTVTRLVRTSIGNITLSGL
                     SEGSWRPLSDREVRELQAPPQGKKGKNILDNHPKINNSRAVRDNRHHKYSGFKQQS"
     gene            complement(4846..5571)
                     /gene="scpB"
                     /locus_tag="PLQ77_02005"
     CDS             complement(4846..5571)
                     /gene="scpB"
                     /locus_tag="PLQ77_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPL09484.1"
                     /translation="MENLSAVLEALILSSESPLPLEKICAALGSGDKEEIKSALDRLI
                     TEYDERESGIGIQEVAGGFQFRTRPEMASWIKKLKGVKPASLSPAAMETLAIVAYRQP
                     IVKSEIESIRGVDVSAPLKGLLDKKLVRILGRKDVPGKPIIYGTTKKFLEVFNLRELS
                     DLPTMRELRELTEPQESPEQEAISFEDLLPETPAGEMPKAPETPSGPEAKQAEKTPEE
                     DPAADAEEQEEHGVEPNPGDDAV"
     gene            complement(5572..6318)
                     /locus_tag="PLQ77_02010"
     CDS             complement(5572..6318)
                     /locus_tag="PLQ77_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPL09485.1"
                     /translation="MNEETSTDYAIKLDIFEGPLDLLLYLIKKNEIDIYNIPIALVTR
                     QYLDYLNLIKSLNLDLAGEYLVMASTLIHIKSRMLLPVPEEPSPEEMEDDPRAELVRQ
                     LLEHQAFKEAASLLTQRPILERDVFKRSAAIVGNERPGAADDEELVEVSIFELIEAFH
                     QIVSRMDKKDLMEIDLEKLSLSDIINDVMERLTREKNLTFDELLGEKRDRRRIVYTFL
                     ALLELIKLKMIKAYQTAAFGVIRIFPAVEL"
     gene            complement(6327..7319)
                     /gene="trpS"
                     /locus_tag="PLQ77_02015"
     CDS             complement(6327..7319)
                     /gene="trpS"
                     /locus_tag="PLQ77_02015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPL09486.1"
                     /translation="MTRKRILSGMRPTGRLHLGNLHGALGNWVELQNSGNYDCFYFVA
                     DWHAITSEYASTQDIRQNSLYMVMDWLAAGLDPAKSTLFVQSAVKEHAELFLLLSMIT
                     PLAWLTRNPTYKEMQTELSSKDLSTFGFLGYPVLQAADIVMYKAYGVPVGVDQLPHIE
                     LTREIARRFNYLYQKDVFPVPEPLLTQVPKLLGIDGRKMSKSYDNAVYLSDRGKTLHR
                     KIASMFTDPQRMRKKDPGDPDICNVFTFHGLYSPAPDVAEINRECRTAGIGCTDCKKM
                     LAARVEEALAPVHERMDYYQRNIAEIEEMIQKGNDEAAAIARRTMAEVREAVKI"
     gene            complement(7421..7693)
                     /locus_tag="PLQ77_02020"
     CDS             complement(7421..7693)
                     /locus_tag="PLQ77_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL09487.1"
                     /translation="MTKAELIGVMADEAEISQAAAAKALDAYIAAVGKELKKTGKLGL
                     VGFGTFSVVKRKAREGRNPQTGKTIKIPAKKVVKFKAGKTLSDKVK"
     gene            8146..10215
                     /locus_tag="PLQ77_02025"
     CDS             8146..10215
                     /locus_tag="PLQ77_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPL09488.1"
                     /translation="MKDNHLKNEKSPYLLQHAQNPVDWHPWGDEAFTRARLEDKPIFL
                     SIGYSTCHWCHVMERESFEDEDVARMLNETFVNVKVDREERPDIDAVYMEVCKLLTQS
                     GGWPLTIMMTPDRKPFFAATYLPPRSKYGRAGLTDIVGRVGVLWRTRRAELLSSAEEI
                     VNALQKPSAGSARDAGEEELHRAYRLFSMSFDAAFGGFGQAPKFPSPHQLFFLLRYWK
                     RTGEAQALRMAEWTVRNMRRGGIFDQIGFGFHRYSTDERWIVPHFEKMLYDQALMALA
                     CAELYQATGSGEYAAAARETAAYVLRDLTDAGGGFYCAEDADSEAVEGKFYLWTEKEI
                     RAVLDAEEADFFLTHYRHASDRSVPGMREIPEGHFIAHAKPLTDEPGPGQTAFWPVLT
                     EAIREKLFAVREKRVRPHRDDKILTDWNGLMIAALARLAQVLKEPAYAAAAVRAMDFI
                     LRSLRTPDGRLLHRFRGGHSAFAANLDDYAFIIWALLELYEATFDPAYLTRALELHSH
                     LRGFFRDEKDGGFFFTPKDAEPLPVRPKELYDGAIPSGNSVAFLNTLRLSRITGDDAL
                     EKEAREIAGAFGGPAGASPTAFAHFLCGLDFAVGPASEVILAGKEGAPDAQALLGALR
                     RSFTPNKVVIFRPEDTGGADIEAISPRVRSRVCIGGRATAYVCSGFACRLPATDPEQM
                     MALLNGK"
     gene            complement(10249..10602)
                     /locus_tag="PLQ77_02030"
     CDS             complement(10249..10602)
                     /locus_tag="PLQ77_02030"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HPL09489.1"
                     /translation="MIRIQTGFIALLILCLAAADASAFRCGSGMVSAGDTKARVLITC
                     GKPTAKERVSEKNKTSATAVKNKTAKKSGSKSEVWYYNCGSQDYIYALTFEDGVLKKE
                     DTEGKGSGKSDCMGK"
     gene            complement(10628..10948)
                     /locus_tag="PLQ77_02035"
     CDS             complement(10628..10948)
                     /locus_tag="PLQ77_02035"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HPL09490.1"
                     /translation="MKKLRIIGLAVILLCANAVPAAAFRCGSGLVSTGDKKVKVQGTC
                     GLPTTKEIICPRKSSVCPATLEIWYYNCGDNDYIYALTFEDGVLTKETTEGGGVGNSY
                     CLGR"
     gene            complement(10952..11887)
                     /locus_tag="PLQ77_02040"
     CDS             complement(10952..11887)
                     /locus_tag="PLQ77_02040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09491.1"
                     /translation="MGYALNTTDDYHLHGLTSLGWELTLCNALFPKNSPCRGALVREA
                     SFGEHLFHFLNEKIHLTHLKTVLEIGGGMGYLMKDLLTLAPRLHATMADISPVLLQKQ
                     KGSLAGFSAVFLETDFFKIPLTDLRAFDLVILNEVLGDFPTLRAVDGPSSPEAARFAA
                     RIADFEADYNLRFDPAENINIGALEALERLCRAGVPCIYLSEHSCESSHSDPAFPGMN
                     FSPSGNPEKISLKGHAEFTVKFSALEKMARAFGYKVFRGPYTDILPISFNDRVTAALR
                     TPSPLSDRQEILRQFLYDLYKYEYLLLASAPAGKG"
     gene            complement(11995..13035)
                     /locus_tag="PLQ77_02045"
     CDS             complement(11995..13035)
                     /locus_tag="PLQ77_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPL09492.1"
                     /translation="MFYRKILQHRLPITVAAALAGIGIMAYYATCNTGCSYLRGDILG
                     IDLKYVGVGYMGVIILLALFRQADLLRMLVAAGIGVEAFLISFQFREEVFCPFCLAFG
                     ALVILMYLINYERLHMTNARHQKWLYVFGDARIPFTGGRRLPLLSMMIAGYIFVVLTF
                     SGSATPAYAADQAAAPSYGSGPWELMIFTDYFCPPCQAAEKDLEPQLERLLAGGDVKI
                     TFVDFPGHKSSSLYAKYFLFSVAANKGHANSLKARNLLFALAVQKKVDQESVLAAVLK
                     SKNIALRTVDPKPVFQQWLAMIKRFEIDQTPTCILRFSSTYSRKFTGSDQIRDQLVPE
                     LQKRFPGSKRKG"
     gene            13260..15647
                     /locus_tag="PLQ77_02050"
     CDS             13260..15647
                     /locus_tag="PLQ77_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPL09493.1"
                     /translation="MDQDLDNLMALLRKDHPDLSAWIVVCANDRAVDPVRLLIHDRLG
                     GILPRVEGIQSYLNRKIGEKEPLHPVPADEELLYLIQFIAEEFPGEKTPARLAANMLP
                     LITRLAEYQVGKETIAGSERFTSEQWDQLEQYLETAKAFRRWLSRKHLFLAALEASRF
                     DEILPGDREIFIGLPELTPSRERFYRKIRNERLFIDPPLFGPETAAGGPLPFDSAKNL
                     VLSFGKTVRAAQNADIEFLRLAGLHAAADWVTLEVSRFLNDRSESDRLMICLLDESLT
                     PMLWNRSLRWFGNAVNLAVWLPFSVTSAGIRLSAEIHRAGQTGRMPDFRKFAAGQAAE
                     LFANRQRYVREESEALEAAVSISTLLDDWKGRLGRHFAAAAGKFLETKKFRVTGGRSA
                     PVQVVGFGQASGENFSRGLILPVDSGIMPAQPFEGPFINPVHVPFMRKNVFECQDLLF
                     RQILAQCRKAAVAAVDDAIRERTPGYYMSLLSEEFGKPPVKVAFRRPKPDPRDEIRCA
                     VPVDEALRARLKAFTFSYSSLSKILACPFLFYHQYILGVSSPSFMDDDEKINLQMGIF
                     VHRFLQQLSTVGHDRLDDWETFFDELWESRENTELRNTEGVEIYRLNAKLILREIDED
                     ERASGRRVLFAANAISCEEKFTGTLAGRYSITGRSDRLALLSGRAEVLDFKYSKKKSG
                     FALPARETVLERFTEKGILHPAAQLILYSHFIPDVKGARFYFLKETAKDRMMELPKEA
                     LDEAGDLASAIGESLDRIVHGAALAPGDDSRQCEFCRLQSLCGKEGYYRAAGGRR"
     gene            15647..18442
                     /locus_tag="PLQ77_02055"
     CDS             15647..18442
                     /locus_tag="PLQ77_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPL09494.1"
                     /translation="MENVLLKASAGSGKTTQLTREVFERISAGGKFILALTFTRAATA
                     EMRSRIMDQIASTKGMPLLDRMRLILEAGRVGYSTIDSFFYRLFAALGEAPKMADEKA
                     QIDLSRDIEKRFRDGIARSGREKRLIIASRILNTDLDSLWGSLGEEQTLERCAADMER
                     LDDLEDLMKENVLLLRELESLSRAANGLADLPDGLKTRVVANLGDYDRFMSRALCGYS
                     DLEQSRGLGKNIAWHSSPYSDLNAIFKNYRKTAEKLALNKALLRELALAFLCEIYLDA
                     AKAVKEEKGLIFFADVRKALLALDPIASRERPKLMGGYFSLGLDRTEHLLVDEFQDTS
                     TRDMAILLPLIDDILSGPGERGERSFFAVGDWKQMIYGWRGANREALETAIGGYLQNG
                     VIRERSLSRNYRSTPLLVSFFNRLVENLFEGREKTETQKPPEKSGGHRDALRCVSEVS
                     LVELEKDGASEASWYPAIAEYLQKKKEEYGCAWGEMAVLALTNNHVQKIEAELASRGI
                     GVSSVKGRQLLATEEGVAVMLFLTIALSREGETAFAAAAARSPLWKDWFEDAPGIREE
                     FAAQFPRPFGLSAVSLAVERLRGKLSATVLDIWQDEAHAFFSEGGADVDEFLARMFRV
                     RWSVRVPEGENRDSIKVDTIHGTKGLQFAHVFIFWNEDEKSTPFYLPSQKCHVRFSAA
                     ETKCLQAGESTLAGEILEKRDARLMQLRRERANVFYVAATRAVRTLAVFLPVSKSGEY
                     KAVHQAVLNTFAQFAPEGSKSEMVRFSRLPEEAKGSAVWDAPSRHFGKPDPAGEIDPA
                     LISESIQAGIARGERLHRWLARVLDPGRLPPPGELGREEYGAVVRFVRRKEVFELLFR
                     PGRLYVEQPISDQKDFGVVDRMIVSDHAVTIMDYKSGSLRGLRRKYDEQRERYTRIMK
                     SLYPQRNVEYHVLPIDE"
     gene            18530..20692
                     /locus_tag="PLQ77_02060"
     CDS             18530..20692
                     /locus_tag="PLQ77_02060"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HPL09495.1"
                     /translation="MAERKPSQIFLSRGGVLIPTSGGNLQLGIPPETIKDTMRIEGGV
                     PRTFIVPKAMFDVQHGVALAEMEFPVYYNFFFRKSKTRIVCSEKQRKRIETVIAEALF
                     GPAEVDPVLEFACGEYTPGFTDLKAEMSYFRKSMIEGRCADLGDLIEFCVPDRRGKAA
                     FGETEVRFDRRGNIRVYDRGKKIASFGGDAPVMVRKTPLARVSLGFQPPIFGVTTLGA
                     GHGFDPGADTSGLILWVNRRGIMVDPPVNSTEKLAALGVSPKRIDKIILTHCHADHDA
                     GTLQKIFQEGKIHLYTTRTIYGSFMKKAAALTGIEAGLLERLVDFYPVCIGKPVIIGG
                     GAFHFHYTLHTIPTIAIQASLSGKSLVYSSDTMNDPNFIQQLYADGVLTKNRRDFLLN
                     FPWDRDLVFHEAGVPPIHTPMSYLCSLPEDVRKRMYLVHVNPDSVPDGCGLRIAPTGL
                     ANTVDLGASPMPREEAVEMLEAFSGVEWFGQMPLTKAREFLLAASVTHHRASDIIFSK
                     GDAGEAFCIVMSGEIDIVLEDRILTTYDAGGYFGEKCLFSDEARTATARAKTDVKLLS
                     IRKEEMLNLIRGTAAESLLRQIADFQNVELRDAVKDNQILDSLSSTQKTRLHGLIRPF
                     PGTLHTGDVIASKGSDARCVYMIREGRVDVYHRRSRIATLSRGDLFGVKKIFRPKEKN
                     FSFVAGEKTALYFIEQDGLRRYMEDNPGVYVKLYHSPY"
     gene            20802..21953
                     /locus_tag="PLQ77_02065"
     CDS             20802..21953
                     /locus_tag="PLQ77_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09496.1"
                     /translation="MQSSDIQRLFHEHGRKVVLQEGDVLFCQGEESDAVYFVAGGRLG
                     VFAAQSSSEDVQINTIEAGEMVGELGAITRHPRTATLKAASPRVELLSIATPDFRALL
                     SDSLSLVEAMTLTNREHLMDADRARIQFGTSNLQMRKRVASLGQEKAQLQELLQLREE
                     LEAMVVHDLRNPLNTVIMALGLVERMKDEVKDPETFALAMRLAKGAANRMNRLIATLL
                     DIARLEAGKLALSIAQVDLNTLFEEVIEMEKAGVTKSVEIKAAAVPGLTVKADREVLW
                     RVMSNLLGNALKFAPEASTIVVEAQRGDDRKILISVTDAGPGIPEGERERIFEKFTRV
                     KNADGGAYSGTGLGLTFCRMAVEAHGGCIWVEEGPSGVGSRFCFQLPQD"
     gene            complement(21977..22573)
                     /locus_tag="PLQ77_02070"
     CDS             complement(21977..22573)
                     /locus_tag="PLQ77_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPL09497.1"
                     /translation="MKAFAKAGLVLLAVWLALAGCSPKSYVVLMENQDGTTGKLAVSG
                     EKKETLLNQPRTAASLDGKSEKPFLVDEQKIQKDFAVALAAQPPLPVSFMLYFEAGGA
                     LLTNDSKALIPSIVEAAMKYPAPDVSVIGHTDTMGNRDYNEQISLQRARTVAQIIRDA
                     GIQVHDLTIESHGERNLLIPTPDNTPEPRNRRVEITVR"
     gene            complement(22592..23029)
                     /locus_tag="PLQ77_02075"
     CDS             complement(22592..23029)
                     /locus_tag="PLQ77_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPL09498.1"
                     /translation="MKRFAAVFIALFWTLTPAWAADTIGYVKTASGKAEVIDAGKAVS
                     ARVGTPIQMGNLLKTGPDGSLGITFRDNTVMSFGPDTELTVDEYLYEPGKDRLKFGTS
                     MTKGTLQVISGVIAKLKPEAVQMKTPAAVIGCRGTRFLVKVEP"
     gene            complement(23058..25199)
                     /locus_tag="PLQ77_02080"
     CDS             complement(23058..25199)
                     /locus_tag="PLQ77_02080"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL09499.1"
                     /translation="MRFFPKLSPGLLAPFSAVFLGLAVFLAEPLPLQVLRNAVFDQYQ
                     RWHPRPYQAAPVRIIDIDEESLQRLGQWPWPRTRMARLIEKLRDAGAGVVALDIMFAE
                     PDRTSPERITGDWELPREIRRRLEAMPGHDAILADTLAEGRVVLGFAAEQGLARDHGP
                     ARPFRFVFSGESPLPHLYSFSGAVTSIPPLEKAAAGNGAMNFIPDSDGVVRRVPLIIK
                     IQEQAFPSLAAESLRVVQGAHNYIVRTLPEKGAGIQEIRIGSLTVPATPRGEIWVHYT
                     KSVPDRFIPAWKVLAGEAPREKLEGHILLVGASAQGLMDIRFSPMGTVMPGVEAHAQA
                     LEQILTKTYISRPSWAAAIEGLTILAGGLAVGIIALSAPALVSACITAAILFAAGWGA
                     WIAFVRYHLLLDPVTPGLALLAAFILGSIIHHRTSERRHRWVREAFSRYISPNRVDYL
                     VRHPDQLELGGRRQECSFVFTDLAGFTGLMEKMDPADAVSVLNAYLDAMVTIAFGRGG
                     TLDRIVGDSVAIMFSAPVAQPDHRARALSCALEMHAFAFGYAADCRAKGIELGQTRIG
                     VHSGEVIVGNFGGKTLFDYRALGDAVNTASRLEGANKYLGTLICVSEATLQGCPEAVV
                     RPAASLVLKGKTQPISVYEPLAAPEANGGKPERDTAYEKAFELLKRREPLALEAFERL
                     AAERPADELVRLHRDRLRSGKKGDTIILEEK"
     gene            25405..25815
                     /locus_tag="PLQ77_02085"
     CDS             25405..25815
                     /locus_tag="PLQ77_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPL09500.1"
                     /translation="MIGVLITTHGNLGNELIHAAESIKGPLKDVMHVCIEQTKGLEDM
                     KKEISNALKKLDHGKGVLILTDLFGGTPSNISLSFMKEGKVEVVTGVNLPMLLKLSDV
                     KEETTLKEFAGFIKDYGKKNISLASEILSKKAIG"
     gene            25820..26332
                     /locus_tag="PLQ77_02090"
     CDS             25820..26332
                     /locus_tag="PLQ77_02090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HPL09501.1"
                     /translation="MAESYDIALVRVDNRLVHGQILEAWVPFVEARCIMVVDDNSASD
                     FFCETVIRMAVPSDIEVNICSVDDFAANYAYVRGSGKKTIVLFGRIADALKAYEQGFH
                     FEALNIGNIYHEDYKVCCAPSVFLCEPEIADIIRLQREKGVAVEVKRVPREKAVDIQD
                     ALKASCDSKP"
     gene            26345..27040
                     /locus_tag="PLQ77_02095"
     CDS             26345..27040
                     /locus_tag="PLQ77_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPL09502.1"
                     /translation="MLTQIVILSLVGGLLCLDRIFLQAMLSRPIIIAPVMGLLLGDAY
                     TGLIIGAIFELFWMDRAPIGIYIPPNDSIASAFAAALAILAGQSLGAVTKELTALSVL
                     LAVPFGILAKKMDVRMMAANDLLSDRALEAARSRDLRAIENMTCLALGRAFVLYAGLL
                     FILQVIFIPLVINLYPKLPVQAGGALTLTYYFLPVLGTAVALSTIKLRGAVPVFCAIF
                     LAAVLAMDYFHVF"
     gene            27030..27524
                     /gene="rimI"
                     /locus_tag="PLQ77_02100"
     CDS             27030..27524
                     /gene="rimI"
                     /locus_tag="PLQ77_02100"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPL09503.1"
                     /translation="MSSDKTGQLFSVDLMKEEDLPEILAIEAASFVTPWTRGMFQDEL
                     RLPHAQCLVIRARQAEKTQVAAYIIFWLVADEVHLHNIAVRGEFRRKGLASGLMNLMR
                     DIAGQAGADRQTLEVRESNTGAINLYRRCGFVVKGKRPRYYDDTREDALVMWAEVRQN
                     GHHE"
     gene            27508..28332
                     /locus_tag="PLQ77_02105"
     CDS             27508..28332
                     /locus_tag="PLQ77_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPL09504.1"
                     /translation="MAIMNKQITIGSVSGNDEIRPGYVLMTIHVHRSFPEPRPGQFVM
                     IRIAGLQEPFLSRPLSIFSFSRGRSHGLVKLLYRVAGKGTQILAGLIRGSQVEIVGPL
                     GKGYTVDPSRENRVLIAGGIGVAPLSLLAQHLCRLSGRGANPAVVYLGAKTADELVGL
                     DQLHKYCNSIRVCTDDGSLGEKSLVTRTFQKDMKKHDPARTSIYACGPKPMLQSLAGI
                     LKDRYFCQVSLEERMACGVGACMGCAVAVRDENGDKTYRRVCADGPVFDLQKVIWE"
     gene            28429..29331
                     /locus_tag="PLQ77_02110"
     CDS             28429..29331
                     /locus_tag="PLQ77_02110"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPL09505.1"
                     /translation="MVVEIAGLKLKNPVMTASGTFGCGEEYAGYVDLNRLGGIVVKGL
                     SLKPRLGNPPPRIMETAGGMLNAVGLQNIGVDAFIEEKLPFLRTYDVAVIANIYGESC
                     AEYAQVARKLSAAPGVHALEVNVSCPNVKKGGLSFGADPKAAAEVTKRVKAETHLPVI
                     VKLTPNVTDITVIARAVEEAGADAVSLINTLTGMSIDLKTRTPHLKNITGGLSGPAIK
                     PVALRMVWQAAQKVTIPVIGMGGIMTAEDALEFMVAGAAAVQVGTANFINPLATSEIV
                     QGMRQYVKHHKLKNITEIVGTLKV"
     gene            29573..30259
                     /locus_tag="PLQ77_02115"
     CDS             29573..30259
                     /locus_tag="PLQ77_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09506.1"
                     /translation="MRRGTEEILSGVYLIGGPNVTQADDAAVYLIDFKGELVLIDAGA
                     GGSASQLVRNIEMLGLNPALVSHVILTHCHIDHIGAAPFFREKYKAKIVIHEMDAKAL
                     ETGDSVRTAANWYGTTFPPTKIDRKLTGARETLKFDNDELHCLHTPGHTPGSIAVYLD
                     RSGQRVLFGQDIHGPFHKAFGSNIEAWKKSMQTLLALEADILCEGHFGIFRPKNKARA
                     YIERYLEEYE"
     gene            30307..31041
                     /locus_tag="PLQ77_02120"
     CDS             30307..31041
                     /locus_tag="PLQ77_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPL09507.1"
                     /translation="MRKTLFVFFMLFWAAGITGAAQAGIFDDILKKLPAAKSAAPSAG
                     LDQKTTISGLKEALVISSKNAVKAVSKKDGYFGNQVVKILLPDNIQKAADVVAKLGYQ
                     KQVDHLILSMNRAAEAAAPKAIPLFADAIKSMSIDDARKILQGGDAAATEYFKEKTSA
                     KLYEEFKPVVVANMNKVGVAKAYKNMTAPYEALPLVSSKSIDLDRYVTSKALDGLFLI
                     IAEEEKKIRTDPAARVTDLLKTVFGK"
     gene            complement(31227..32036)
                     /locus_tag="PLQ77_02125"
     CDS             complement(31227..32036)
                     /locus_tag="PLQ77_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL09508.1"
                     /translation="MLILKGKNVLITGAASGIGKALAECFAREGSNLFLGSHPREEDQ
                     LCRWADYLEHQYHIKTKKFPVDLSLASGPELLYRQVEQTGKTIDILVNNAGIMLYGDF
                     ANLPLDGQLEVITVNAIAYLKLMRLFLPGLIEKKSGRILNVVSASAFQPTPHHAVYGA
                     TKAFVQSLSEAVGQEIKSSGIKICTLNPSYTDTPMLRGKGFPKKLWWYSISGLSSPEN
                     IARKGVKAFKKGKPIYIPGIQNWFAASVLTRVFPRRLASYVPSLVLREKGN"
     gene            complement(32177..33415)
                     /locus_tag="PLQ77_02130"
     CDS             complement(32177..33415)
                     /locus_tag="PLQ77_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL09509.1"
                     /translation="MGNVYIIGVGMIRFNKYPDKTVISMAHEATDLALKDAGLAREDL
                     DAVFFSNTFWGMFDNQHSIRGEVVMRRMGVGKVPVVNVENACAGGSTALHLAYTGIRS
                     GMYDVAMAIGSEKITNPNKMLSLSAYGTCMDVGNFEQHIQNLMEISKTFTVKIPAGQS
                     APGEGRSIFMDAYAMGARWHMSRFNSTQQQLAVICAKNHLHGSLNPLSQYQQVMTAEE
                     VLADKMVAYPLTRAMCAPVGDGSAATIICSEKFLKKMTSARPVQIRASVLGTGADRDL
                     NAPDIGERLAKQAYHMAGVGPEDIDLAELHDATSWGELHQTESMGFCPMGEGGPFAES
                     GATTLGGKKPINTSGGLECKGHPIGASGLSQIHELVMQLRGEAGKRQVEGAKIGLAEN
                     GGGNIGVEEAAMCIHILEKM"
     gene            complement(33408..34652)
                     /locus_tag="PLQ77_02135"
     CDS             complement(33408..34652)
                     /locus_tag="PLQ77_02135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL09510.1"
                     /translation="MAYRDLNLDLTDEHIALKESVHKFSMEVLRPASIELDKLSPEEV
                     IAKDSLYWKCMRQMYKNKYHTALISDAYGGMGLDPLSVHIFFEELAYGSAGFTVSLGV
                     SIFSAFYACMVPDDHLVNKFIIPFVECEDASIMSCWSITEPEHGSDILMPGTPFFHDP
                     KITQQVKAKKKNGDWIINGQKAAWVSNGPIASNAALFVNIDSAQGMSGGGICLIDLTQ
                     PGVTRGKPLDKMGQRELPQGEIFFDDAICPGEDMIIDPESYEMMTDITLATANACMGA
                     FFTGVAQAAYDLAVQYCKERVQGGKTISNHQWVQKKIFDMFSKVQAARQLSRAAMIYN
                     MNTTPPAVPYSVAAKVFCTNAAFEVASDAVQLFGGNGVSKEYPIEKLFRDARASMIED
                     GSNDSLAIGIGTALLKEEYANG"
     gene            complement(34727..36340)
                     /locus_tag="PLQ77_02140"
     CDS             complement(34727..36340)
                     /locus_tag="PLQ77_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL09511.1"
                     /translation="MASKAHLNQVIASHLIEIRADEMPDKEIYIFENGEHGEDILTYR
                     ILHERSNKIARLFMDKGIGKGDTFAIYMRNHPEFVCGMMAGITLGAIAVPVDPRNKRQ
                     RLKFLLNNCKARAVIVSGECLEDLEAVITDVPTLRFITVAYQPGQNAAVSAKHHALNE
                     VMEKDTWKPVEQQIMDVRHPMQIIYTSGTTGDPKGVMIRNNRTGLFNILNSIVWKYKS
                     DDILYNGLSLTHGNAQAVTLFPALSLGVKAVFSPRFTKSRIWDICRKYGCTSFSLLGG
                     MAAGIYNEPEKHDDGDNPVKVVISAGTPMAIWEGFEKRFNVQILEWYGAVEGGFAYKP
                     TGQGPVGSFGKPVPGVMEFKVVDDHDNPVPAGKTGELISRMIRGDTKVDYLGLPEESQ
                     DKTKGGWLRSGDMVHKDENGWYFFDFRKGTELRRFGDFIQPDFVESIIGRHPDVSEAC
                     VYGIPAASGSPGESDLVAAIAPFEDSVIDPASVYQKCKNELQGNFIPSYLHVVKEIPK
                     TITEKALTRVLREMFEKKEGDIYKYEDFK"
     gene            complement(36357..37025)
                     /locus_tag="PLQ77_02145"
     CDS             complement(36357..37025)
                     /locus_tag="PLQ77_02145"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HPL09512.1"
                     /translation="MAIRDMNDLVQSGLDRSIELMTVAAHNSFRFNNKNTVKIVSLGK
                     EEIASIGEFCYSLADMSPLAARDGRFVMELIKEPCGLLLLGDKRKSDFNYNCGACGYK
                     TCAELNKAEVVESLTAWGPSCQFKNININIATNAAASMAWKLGLHCRVFSTLGMGAFA
                     LKMIEDVDLIVSVSVSAAKSDPYFDRHKYWTKEHWDETFQKEFPTFTRGFIGAVEDEG
                     TFKI"
     gene            complement(37054..37731)
                     /locus_tag="PLQ77_02150"
     CDS             complement(37054..37731)
                     /locus_tag="PLQ77_02150"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HPL09513.1"
                     /translation="MSIEKSMKQFEQDRLEAVRIASRLLLASGTTTPRVGGVGECTIH
                     IIDDPCAIEDLCLGMESMSRENKAWDFFKRDAAMLRDADAVLVVTSLRCVNDPADINC
                     NMCGKMTCESLRETERLPDDADVAFKGPLCIFRANNISYAIDGIISQARNLGIDYGVF
                     WSVGAAAMRMKILPHATGFALAVAVSITEKSPFRDIPLKYGEINEHTMNDRIIKRLWP
                     QFRSIYS"
     gene            complement(37760..38146)
                     /locus_tag="PLQ77_02155"
     CDS             complement(37760..38146)
                     /locus_tag="PLQ77_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09514.1"
                     /translation="MAVFANTEKMYEVLGTLFNTLMNHPSVGPKFIESNITIKFTIND
                     PGGTIWLTPEKGVICGDADLKPTIDMTLSGDTCHQFWLQEVSMPIALAKGLIKAKGPL
                     PKVLKLLPLLKPAYEAYPEISRKYGL"
     gene            complement(38565..39176)
                     /locus_tag="PLQ77_02160"
     CDS             complement(38565..39176)
                     /locus_tag="PLQ77_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL09515.1"
                     /translation="MSSNAQREVLRRAQIVDAALREISAKGMNNITVESIAKAAGLSK
                     GGVTHYFKSKDDICEAVFQSFFDFIFQRSKHNIDLYSDPLSKLLSFGWLYNWGDPDVS
                     TGYPLLFDFMAEACHKESYRAIFHEWVNNWVALLKDAIIEGNNDGTFNCSDPDATAKA
                     ISAVYHGLAVRWYLDRDSHSTQWTITSFTDAINGLLTGSLNKI"
     gene            complement(39366..40574)
                     /gene="hemW"
                     /locus_tag="PLQ77_02165"
     CDS             complement(39366..40574)
                     /gene="hemW"
                     /locus_tag="PLQ77_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPL09516.1"
                     /translation="MIKNEQAGLYIHVPFCLSKCGYCSFYSVVSPDLIPDFVKAVVRE
                     MAFYKPSFVPFKGFDTIYLGGGTPSLLSAKQLDDLLTAARHHFRFDDPGSNHPLRHTG
                     EYPEGHKCRYPENIEITMEVNPGDVSKNYLRDLKAAGVNRLNIGVQSFDDRILKFLGR
                     RHSAKEALAAIDEARNAGFQNIGLDLIYGVSGQDIRLWLKTLEQALSFAPEHLSCYQL
                     SLEEKTPLYKQYSKESLQLPAENEALDFFMNTSKILTDAGLIHYEVSNFARSDSLKSR
                     HNMKYWRHAPYLGLGPAAHSFLDSRRWWNKADVKAYLNEVGGGKKPVDKSESLSAEQL
                     ALEALFLSMRTNDGIHLQQYKNRFGADLLVGKKQVIDELMENGLLELKDGRLQPTLAG
                     MAVADSLALM"
     gene            complement(40567..>41909)
                     /gene="lepA"
                     /locus_tag="PLQ77_02170"
     CDS             complement(40567..>41909)
                     /gene="lepA"
                     /locus_tag="PLQ77_02170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPL09517.1"
                     /translation="LDPDTHIKASAKEGTGIEEILEAVVERIPPPTGSGDNPLAALIF
                     DAKYDPYRGTIIHCRVFEGGVKAGDIIRFMYNNSTYKVEEVGHFLLQRAKREKLSAGE
                     VGYIIAGVKTVSDVRTGDTITHQERPCPQPLPGFKEVKPVVFASIYPIASDDYQDLAD
                     SLEKYKLNDASFIYEKDSSAALGQGFRCGFLGLLHLDIVQERLEREYDLSIILSVPSV
                     RYRFTLEDGRVLYVDNPTHYPDPAEIDKGEEPYIRASMMMPERYLGAVMKLCMEKRGV
                     NSSMNYTGPGRVELSYEMPLAEVIFDFYDRFKSVTQGYGSFDYNMIDYRESSLVLMDI
                     LVNGEKVDALSQIVHRDRARARALLACDRLKDEIPRQMYKIAIQGAIGGEIIARSTIS
                     AFRKDVTAKCYGGDITRKRKLLEKQKAGKKRMKMIGSVSIPQSAFLAVLKSDND"
BASE COUNT         8789 a        11450 c        12087 g         9583 t
ORIGIN      
        1 ctgttttgcc attctggtca tcagcctcaa aaccatgaag gccaaactgg caacggtggg
       61 catcatttac gccgtgcccg taatcatgaa cgtgctggcc ttcggcttct ggggctggaa
      121 ttattgatgc cggcgagcct tcaggccgcc cggctgcgga gtaggcgaat gcgcctgtgt
      181 gcaaaaaaaa tgcgtttgat gttgttcatc ggactggtgt ttttcctgtc ctgcgcgccg
      241 gccgaagaac cggcagcgaa ggacggccgg ctgaccgtga tcgccacgat ttttcccctg
      301 tacgactttg cccgcaatgt cggcggggat aaggtggcgg tatccatgct gctgccgccc
      361 ggctcggacg cccatcacta cgaattaaag cccagagaca tcgttcgggt cagcaaggcg
      421 gacatttttc tgtttaccag ctttgaactg gagcagtggg cctataagat catcaacgcc
      481 gccgccgaga aaacaaacat gctggcggtt gaaaccggcc ggggcgcgtt tctgctgccc
      541 ctgccggagt cgccggatca tgaccacggc gccggggcgg ccggggggat cgacgaagcg
      601 gagtacgcgt cgcgatacga tccgcacatc tggctggacg ccgccaacgc gcagaaaatg
      661 atcgacaaca tcgcggcggc tttcatcacc cgggacccga aaaacagcga gacctatcgg
      721 aaaaatgccg aaggctacaa acgccggctg atggacctgg atgaaaaata tcgcgagggg
      781 ctttccgcgt gcagcaccag aacccttttg catgcggggc actgggcttt cgcgtatctg
      841 gccaaccggt accggcttca ctaccggtcg gcgtccaaca gttccgcgga cgcggagcct
      901 tcccctcagc agatcttgtc gctgatggaa cagatcagga aagagaagct gcgctatatt
      961 ttttacgaag acttcacggc gccgaagctc gcggcggtga tggctgacga aacgggcgtg
     1021 ggtctgctca agctcaacaa cggacacgat gtcggcagga aagactttca gtccggcgaa
     1081 acgtatctga cgcttatgga aaaaaatctg gctcatctca gagaaggttt gcgatgccgg
     1141 taattcgcgt agaagatttg acctaccgct acaacggggc ggaagtgctg agcgggatcc
     1201 attttaccgt ggaaaggggc gtctatctgg ccatcgtggg tcccaacgga tcggggaaaa
     1261 ccacgctcgt caaaaatatt ctggggatcc tgaagccgca gcagggaagg gtggatctgt
     1321 tcggcacaag gatcgaggat ttcaaccaat gggaaaaaat cggctatctg ccccagcggc
     1381 tcagcggttt gaatacacac tttcccggca cggtgacgga agtcgtgcag atgggcctgc
     1441 cgaaaaaaga cccgggtctg ctgtcgcgca cactggacgc gatggcgatc ggccacctgg
     1501 cccggcgttt gatcggcgag ctgtcctatg gcgaacagca gcgggccatg ctggcgcggg
     1561 cgctgatccg gcggccgcag ttgctgatct ttgacgaacc gaccaccgcg ctggaccccg
     1621 aaacccgggg cattttctac gggctgaccc aggagctgag ccggaaagaa ggcgcaacgg
     1681 tggtgctggt gacccatgac agcggcgtgg tcggtcaata cgctcagcat ctgctgtatc
     1741 tggacaagaa attgatcttt tccggaacat tccgggactt ctgcgcttcg gtggacatgg
     1801 agggattttt cggcccggcc agtcagcaca tcatctgcca ccagcatgat ttgaagggga
     1861 gcggccgctg atggatctgc tcgatgtttt cacgtatgga ttcgttcagc gggcgatgct
     1921 tgccggttcc ctgattgccg cggtttcggc gctgctgggc ctgtttctcg ttctgaggcg
     1981 cttctccctg atcggggacg gcctggcgca taccaccttc ggcagcgtgg cggtcgtgct
     2041 gctcgtcggc atcagcccgt tttacgttac gctggcggct ctgccgctgg tgatgatttc
     2101 ctcgctcgcc atctaccggc tgacccaatc gagaaagatt catgccgatg ccgccatcgg
     2161 catcgtttct tccctgggaa tcgcggcggg catcattctg tccagccttt cgggcggctt
     2221 caacgtggat ttgttcagtt atctttttgg aaatattctg accgtgaacc agaccgaact
     2281 gttgctttcc gtgatcgtct ttgcccttgt cgcggcggcg gttgtttatt gctacgaaga
     2341 tctgttcgcg gtgacctttg accaggagct tgcccgcacc atgggcgttc aaaccggcag
     2401 gatcaacttc gttctttttc ttctgacggc cgtatccgcg gttctggcca tgaaagttgc
     2461 cgggatcatg ctggtttcgg cgctcttgat tctgcccgct ctcaccgcgc tgcagctctc
     2521 ggtcagtttc cgaaagacga tgatgtccgc cgtggggttt gcggtcgggg cggtgctcct
     2581 gggaattgtg tttgcctttg tcctgaattt gccggcaggc gccatgattg tcctgttcaa
     2641 tatcgttttt cttctgctgg ttgccggtgt ccggaaattt tgcgccgggc gcaaggcatg
     2701 atcccgctcc gatgaggacg gccatgaggg tatccccttt tctttacatg cttgccattg
     2761 tggcgggcct tttgctgcct caggcggccc cctgggcttc gcttctcgtt ctgccggcgt
     2821 tgacgattgc cctcacggtt gcgctgcttc gctttccggg gggattcttc aaaaatccgc
     2881 ggacgctgct tcccggggct ctctggggct gcctgatgaa ctacctggtt ctggggaacc
     2941 tgatgattct gggcggaatt ttcctgatca agaatgagaa tttctggatc ggcctggtta
     3001 tcctggccgc cgttccttcc gctctggcgg ctcttccctg gagcgaaaag ctcggcgcgg
     3061 atcacaggct gacgctgacc ggttttgccg ggacctacct gggcgcgctc atcctgattc
     3121 cgcttctcgc ggcggctttt ctccggtaca tccccgtgtg ctgcgaaaag ctgatcatca
     3181 tggccgccgc gctgatattc cttccgctgt tgctgtccag ggtcgcggtt gaccgcaact
     3241 gggatgaacg gtttcaccag cgcatgggga ccgtttctga tgtctgcttt tttatcgtgt
     3301 tttacgccgc cgcttccgcc aattccgtcc tgatccggca ggggagcctg gacatgcttt
     3361 tcattctggc gctggctttt gtttccacgg ccctgatcgc cgccgcgctc atcgcggccg
     3421 gcagggtttt caaactgtcc cgccccgtgg tttcatcgct tctgcttctg ggaaccatga
     3481 aaaattgcgg ccttgccgcc ggcatcgccc tgtatgccgt gaacgacgaa gctgccgttc
     3541 cggctttgat tttttccatt tttatatttc tgaacggatt gtggctgtgg ctttggcgca
     3601 aaggcctgga aaggagattt tatcgggcct gaactgaaac gcaaggagag ctcatcattt
     3661 ggcaaaccaa aatgaagggg gcttgtttcc aagtcccctt cattaagtat aacaaacact
     3721 tgcaggattt ctatttgttt ttgtcgaccc tgtccttcag ctctttgccc actttgaaaa
     3781 agggcagttt ttttggttca accttgattt ttgttccggt tttgggattt cttcccgtgt
     3841 aggatccgta ttctttcacc acaaagctgc cgaatccgcg aatttcgatg cggcctcctt
     3901 ttttcaactc atcgttgaag cctttgaaaa ccagattgac cacgtcaatg gcttttttct
     3961 ccgtcagatt catcttcttg cttaaagctt caataagacc ggatttattc atagatcctc
     4021 cttaagactg ctgcttgaaa cccgagtatt tatgatgccg attatcccgt acggcgcgac
     4081 tattatttat ttttggatga ttgtcaagaa tatttttacc ttttttcccc tgcggcggcg
     4141 cctgcagctc gcggacttcc cggtccgaaa gcgggcgcca tgagccttcc gacaggcccg
     4201 aaagcgttat gtttccgatg gaggtgcgca ccaggcgcgt aaccgtgaaa ccggcagcct
     4261 caaaccctct gcggatgatc cggtttctgc cctcgtgcag ggtcaggctg atccaggcgc
     4321 ttttgtcgtt gaccttttcc tgcttcagat tttccggttt gaaaacaccg tccggcagca
     4381 aaatcccctt gctgatttcc tttaatttct caaggcttat gcgaccggca atctttgccc
     4441 ggtaggtttt ggggacccgg aagccagggt gggaaatttt ttgagcaaac gctccgtcat
     4501 tggtcagaag cagcaggccc gcggaatcat aatcgagccg gcctaccgga tatacccttt
     4561 ccggaacatc ccggaccaga tcgacaaccg tcggtctttt ctgcggatcg gacagcgtgg
     4621 tgacgtagcc cgccggtttg tacaacgcaa tgtagcagcc cggattttca atggagatga
     4681 ttttgccgtc aatgcggatc acgtcttttt gcggatcggc tttagagccc agttcgcgca
     4741 cgatctctcc gttgacgctc acccggccct ccgtgatcat ggcttcggca tgacggcggg
     4801 aatcgatgcc cgcggcggag atgattttct gaaggcgttc tttcatcaaa cggcatcgtc
     4861 cccggggttt ggctcaaccc catgttcttc ctgttcttcc gcgtcggctg cggggtcctc
     4921 ttcgggggtt ttttccgcct gctttgcttc cggacccgaa ggcgtctcag gagcctttgg
     4981 catttcccct gcaggcgtct ccggcagaag gtcttcaaag gaaatggctt cctgttcggg
     5041 cgattcctga ggttcggtaa gctcccgaag ctctctcatc gtcggcaggt ccgaaagctc
     5101 cctgagattg aaaacttcca gaaatttttt ggtcgttccg taaatgatcg gctttcccgg
     5161 cacgtccttg cgcccgagga tgcgcaccag ctttttgtcc agcaggccct tgagcggagc
     5221 gctgacgtca acgccgcgaa tgctttcaat ctccgacttg acgatcggct gccggtaggc
     5281 cacgatggcc agtgtttcca ttgccgcggg cgacagcgag gccggtttga ctcccttgag
     5341 ttttttgatc caggaggcca tttccggccg ggtgcgaaac tgaaaaccgc ccgcgacttc
     5401 ctggatgccg attccgcttt cgcgctcgtc gtattccgtg atcagacgat ccagggcgct
     5461 tttgatttcc tccttgtccc cgcttccgag cgcggcgcag attttctcca gcggcagggg
     5521 cgactccgac gacaggatca gggcttcgag aacggctgaa aggttttcca tttacaactc
     5581 cacggccggg aagattcgaa tgacgccgaa ggccgccgtc tgataggcct tgatcatctt
     5641 cagcttgatg agctccagca gggccagaaa agtgtaaacg atccggcggc ggtctctttt
     5701 ttcccccagc aattcgtcaa aggtgagatt tttttcgcgc gtaaggcgct ccatcacgtc
     5761 gttgatgata tccgaaagcg acagtttttc caggtcaatt tccatcagat cctttttgtc
     5821 catgcgggaa acaatctggt gaaaggcttc gatcaattca aaaatgctga cttccaccag
     5881 ctcctcatcg tctgccgccc cggggcgttc gttgccgaca atcgccgcgg agcgcttgaa
     5941 cacgtcccgc tccaggatgg gcctctgggt cagcagggag gcggcttcct tgaaggcctg
     6001 gtgctccagg agctggcgga ccagttccgc gcgcgggtcg tcttccattt cttcgggcga
     6061 cggctcttcc ggaaccggca gaagcatgcg ggacttgatg tgaatcagcg tggaggccat
     6121 gacgagatat tcgcccgcca ggtccagatt gagcgacttg atgaggttca gataatccag
     6181 gtactggcgg gtgaccagag caatgggaat gttgtaaatg tcaatctcat tttttttgat
     6241 cagatacagc agcaggtcca gcggaccttc gaaaatatcc agcttgatcg cataatctgt
     6301 ggaggtctct tcgttcatag gtcgcttcag attttgacgg cttcgcgaac ttccgccatg
     6361 gtccggcggg cgatcgccgc ggcctcgtcg ttgccttttt ggatcatctc ctcaatttcc
     6421 gcgatgttgc gctgatagta atccatgcgc tcatgaacgg gcgccagcgc ttcctcaacg
     6481 cgcgccgcca gcattttctt gcagtcggtg cagccgatgc cggctgtgcg gcattcccgg
     6541 ttgatttcgg ccacgtccgg agcgggggag taaaggccgt ggaaggtgaa tacgttgcag
     6601 atgtcgggat cgcccgggtc ttttttccgc attcgctgag gatcggtgaa catggaggcg
     6661 attttccggt gaagcgtttt gccccggtcg gacaggtaaa cggcattatc gtaggatttg
     6721 ctcattttcc tgccgtcgat gcccagcagc ttcggcacct gggtcaaaag cggctccgga
     6781 acgggaaaga cgtctttctg gtaaaggtag ttgaagcgcc gggcgatttc gcgggtcagc
     6841 tcaatgtggg gaagctggtc cacgccgacc ggaacgccgt aggccttgta catgacaatg
     6901 tcggccgcct gcagaacggg gtagcccaga aagccgaagg tggaaagatc tttggagctc
     6961 aattccgtct gcatttcctt gtaggtggga ttgcgcgtga gccaggccag cggcgtgatc
     7021 atggaaagca gcaggaacaa ttcggcgtgt tccttgaccg ccgattgcac aaagagggtg
     7081 cttttggccg gatcgaggcc ggcggccagc cagtccatca ccatatacag gctgttttgc
     7141 cgaatgtcct gtgtcgatgc gtattcactg gtgatcgcat gccaatcggc gacgaagtaa
     7201 aagcagtcgt agttgccgct gttctgcagt tcgacccagt tccccaacgc gccgtgcaaa
     7261 tttccaaggt gcagccttcc tgtgggccgc atcccgctga gaatcctttt tcgtgtcaag
     7321 ccagacgctc ctcgtttcca aaatggataa atgcaccaag gcctccggtg tttcgaaaag
     7381 ctgacggagg ccttggttaa aaggatgact taaaaaagcc ctatttcact ttgtccgaca
     7441 aagtcttgcc ggccttgaat ttaaccactt tcttcgcagg aatcttgatc gtcttgccgg
     7501 tctgaggatt cctgccctct cttgcttttc tcttgacgac ggagaacgtg ccgaaaccga
     7561 ccaatcccag cttgccggtt tttttcagtt ctttccccac cgcggcgatg tacgcatcca
     7621 gcgctttggc cgccgccgcc tgggatattt ccgcctcgtc cgccattact ccgatcaatt
     7681 ctgctttcgt catgctccag tccccctttc ctgaatttat atggttaaat tttcattagc
     7741 cgcccggcgc ggctgtgatt tgcctctggg atccgggctc ccgtgcttga cgccctgatg
     7801 aaggtcaagg ggcgcgatct tcaggaccgg ggccgaatgc ttcatgggaa tgaatggaac
     7861 cctgttgttt gtactgttga cgaagcgcgg cacgcctcaa accggcgcta gggctaacac
     7921 aaaaaaaaac aacaatcaag aaaaaagtat ttttgcggcc ggttttcacg ggcgatttcg
     7981 cgggcggcag ggcgctttgc agacggcggt gaaacaggcg catgccgtgc gtgaacgccg
     8041 tctccggccc gagccgtctt ctttgacaag cactcccgcc tgtgctacga atccacacgc
     8101 gccgcagatg aacgaatgcc ggcgagccga aggggagcaa gggcgatgaa agacaaccac
     8161 ttgaaaaacg aaaaaagccc ctacctcctg cagcacgcgc aaaatccggt ggactggcat
     8221 ccctggggtg atgaggcttt cacccgggcg cgccttgaag ataaacccat atttctttcc
     8281 attggttatt ccacctgcca ctggtgccac gtcatggaac gggagtcgtt tgaggatgaa
     8341 gacgtcgccc gaatgctcaa tgaaacgttt gtgaacgtca aagtggaccg cgaagagcgc
     8401 cccgacattg acgcggttta tatggaggtc tgcaaacttt tgacgcaaag cggcggctgg
     8461 ccgctgacga tcatgatgac gccggatcga aagcctttct tcgccgccac ctaccttccc
     8521 ccccgatcga aatacgggcg ggccggcctg acggacatcg tcggccgggt cggggttctg
     8581 tggcgcacaa ggcgcgcgga gctgctttcc tcggctgagg agattgtaaa cgcccttcag
     8641 aaaccttccg caggcagcgc gcgggacgcg ggggaagaag agctgcaccg ggcctaccga
     8701 ttgttttcca tgagctttga tgcagccttc ggcggattcg ggcaggcgcc gaaatttccg
     8761 tcgccgcatc agctcttttt cctgctgcgc tactggaagc ggaccggcga ggcgcaggcc
     8821 ctgcgcatgg cggaatggac ggtgcggaac atgcgccgcg gaggcatctt tgatcagatc
     8881 ggcttcggat ttcaccgata ttcgacggac gagcgctgga tcgtgccgca ttttgaaaag
     8941 atgctgtacg accaggcgct catggcgctg gcctgtgccg agctatatca ggcgacaggc
     9001 agcggggagt acgcggcggc cgcgcgggaa accgccgcct atgttttgcg ggacctgacg
     9061 gatgccggag gaggattcta ctgcgccgaa gatgcggaca gcgaggctgt ggagggaaag
     9121 ttctatctgt ggaccgaaaa ggagattcgc gccgtccttg acgccgaaga ggcggacttc
     9181 tttcttacgc actaccgtca tgcaagcgac cggtccgtgc cgggcatgcg ggaaattccc
     9241 gaagggcatt tcattgcgca cgcaaaaccg ctgacggatg agcccgggcc gggccagact
     9301 gctttttggc cggtcctgac ggaagcaatc cgggaaaagc ttttcgcggt tcgggaaaaa
     9361 cgggtgcgcc ctcaccggga cgacaagatt ctcaccgact ggaacggcct catgatcgcc
     9421 gcgctggcgc gcctggcgca ggtgctgaag gaaccggcct atgcagcggc cgcggtccgc
     9481 gccatggatt tcatcctgcg atcgctcaga acccccgacg gccgcctgct gcaccgtttt
     9541 cgcggcgggc atagcgcctt tgccgcgaac cttgacgact atgccttcat catctgggcg
     9601 cttctggagc tttacgaagc aacttttgac cctgcgtatc tgaccagggc gctggagttg
     9661 cattcgcacc tgaggggttt tttccgcgat gaaaaggacg gcggcttctt ttttactccg
     9721 aaagacgcag agccgcttcc ggtccgtcca aaggagcttt acgacggcgc aatcccgtcc
     9781 ggaaattccg tcgcttttct caatacgctg cgtctgtccc ggatcaccgg ggatgacgcg
     9841 ctggaaaaag aagcgcgcga gattgccggg gccttcggcg ggccggccgg ggcttccccg
     9901 acggcctttg cgcactttct ttgcggcctt gattttgccg tgggtccggc aagcgaagtg
     9961 attcttgcgg ggaaggaggg cgcgccggat gcgcaggctc tgcttggggc gctgcggcga
    10021 agctttacac cgaacaaagt ggtcatcttt cggccggaag acacgggagg ggcggacatt
    10081 gaagccatct cgccccgggt tcggtcgcgc gtctgcatcg gcggacgggc gaccgcctat
    10141 gtctgctccg gctttgcctg ccgtcttccg gcaacggacc cggaacagat gatggctttg
    10201 ctcaatggaa aatagaaaac ggccgcggtt cctttccgct tccggccgct actttcccat
    10261 acagtcggat ttaccgctcc cttttccttc cgtgtcctct tttttcagaa cgccgtcttc
    10321 aaaggtcagg gcgtaaatgt agtcctggct gccgcagttg tagtaccaga cctccgactt
    10381 gctgccgctt ttctttgctg ttttgttctt gacggcggtt gcggaagtct tgtttttctc
    10441 gctgactctt tccttggcgg tcggctttcc gcaagtgatc aaaacccgcg ccttggtgtc
    10501 gccggcggaa accatgccgg atccgcagcg aaaagcggaa gcgtcggcgg ccgccaggca
    10561 aaggatgagc agcgcgatga agccggtctg aatccgtatc atatttcttc cccctctcga
    10621 gtgatgttta tctgcccagg cagtaggaat tgcccactcc gccgccctcc gtggtttctt
    10681 tcgtcagaac gccgtcttca aacgtcagcg cgtaaatgta gtcgttgtcg ccgcagttgt
    10741 agtaccagat ttccagagtc gcggggcaga cgctgctttt cctgggacag atgatttcct
    10801 tggtggtcgg aaggccgcag gttccctgaa ctttaacctt tttgtctccg gtggaaacca
    10861 ggccggaccc gcagcgaaag gccgctgccg gaacggcgtt tgcgcaaagc aggattacgg
    10921 ccagcccgat gattcgaagc tttttcatat tctatccttt ccctgccgga gcggatgcca
    10981 gcagaagata ctcgtattta tacagatcgt aaagaaactg ccggagaatt tcctgcctgt
    11041 ccgacagcgg agacggcgtc cgaagggcgg ccgtcacccg gtcgttgaac gagatcggca
    11101 gaatatcggt gtaggggccg cgaaacacct tgtagccaaa ggcccgggcc attttttcca
    11161 gtgcggagaa tttgacggtg aattccgcat ggcctttgag tgaaatcttt tcggggtttc
    11221 ccgaaggtga aaaattcatg ccgggaaacg ccggatcgga gtgagacgat tcgcagctgt
    11281 gttcgctcag atagatgcag ggaacgccgg cccggcaaag ccgctccagg gcttcgagcg
    11341 ccccgatgtt gatgttctcg gcgggatcga agcgcaggtt gtagtctgcc tcgaaatccg
    11401 cgattctggc cgcgaagcgg gcggcttccg gggatgacgg accgtccacg gctctcagcg
    11461 tggggaaatc ccccaggact tcgttgagga tcacgaggtc aaaggcgcgc agatccgtga
    11521 gagggatttt gaaaaaatcc gtttcgagaa agaccgcgga aaatcccgcg agagatccct
    11581 tttgtttttg cagaagaacg ggggagatgt ccgccatggt cgcgtgaagg cggggggcca
    11641 gggtcaaaag atccttcatc agatacccca tgccgccgcc gatttccaga acggttttta
    11701 aatgcgtcag gtgaattttc tcattcagaa agtgaaaaag atgttcgccg aaggatgcct
    11761 cccgcaccag cgcgccgcgg cagggactgt ttttggggaa aagcgcgttg cacagcgtaa
    11821 gctcccagcc cagggaggtc agcccgtgaa ggtggtagtc atccgtggtg tttaatgcgt
    11881 agcccattga cgaaagccgt tcagcgattc ccggagttct tttccgttct gatcatcgat
    11941 ttcccggata ccggcctgcg acggcaggac aattaggcgg gcatctctcc ggccttaccc
    12001 ttttcttttg gaaccgggga atcttttctg aagctccgga accagttggt cgcgaatttg
    12061 gtccgaaccg gtaaatttcc gggaataggt gctggagaag cgcaggatgc aggtcggcgt
    12121 ctgatcaatt tcaaaccgct tgatcatggc cagccattgc tggaagaccg gtttggggtc
    12181 caccgtccgc agtgcgatat tttttgattt caggacggcg gccagaacgc tttcctggtc
    12241 gacttttttc tgcacggcca gcgcaaagag aaggttcctg gccttcaggc tgttggcgtg
    12301 gcccttgttc gcggcgacgg aaaaaagaaa gtatttcgcg tacagcgaac tgcttttgtg
    12361 gccgggaaaa tccacgaaag tgattttaac gtctcctccg gccagcagtc tttccagctg
    12421 gggctccagg tctttttccg cggcctggca gggaggacaa aagtaatccg taaagatcat
    12481 cagctcccac ggtccactgc cgtaggaagg cgccgcggcc tggtcggccg cataggcggg
    12541 cgttgccgat ccggaaaagg taaggacgac aaaaatatat ccggcgatca tcatggacag
    12601 caggggaagg cgccggccgc cggtgaatgg aatcctggcg tctccgaaaa catagagcca
    12661 tttttgatgc cgcgcattgg tcatatgaag cctttcatag ttgatcagat acatcaggat
    12721 caccagagcc ccgaacgcaa ggcagaaggg gcagaagacc tcctccctga actggaagga
    12781 gatcagaaac gcttccacgc cgattcccgc cgcgaccagc attctcagca ggtccgcctg
    12841 ccgaaacagg gccagcaaaa tgataacgcc catgtatccc acgccgacgt atttcaggtc
    12901 gatgcccaga atgtctcccc gaagatagga gcagcccgta ttgcacgtcg cgtaatacgc
    12961 catgatgccg atgccggcaa gcgcagcggc aacggtgatg ggcagacgat gctgaagaat
    13021 ctttcgataa aacatgggct cgagtctttc ggctttttta atgggcggaa ttatagagag
    13081 ggatgatgag gttgtcaata aaatgttcac ggctgttcag ggggggaagt aacgatactg
    13141 aaatccgggg attcaattgg gtattgacaa ggcccgctcg cttcttcata gtcatggccg
    13201 ggcggaaacg ccttcgtcaa agactaaaga acagaccttc acattcaaag gaagaccgga
    13261 tggatcagga tttggacaat ctcatggcgc ttctgcggaa ggatcacccc gacctttcgg
    13321 cctggatcgt ggtttgcgca aacgacagag cggtcgatcc ggtgcgtcta ctgattcatg
    13381 accggctggg cggcattctt ccccgggtgg agggaatcca gtcctacctc aaccggaaga
    13441 tcggcgaaaa agagccattg catccggtgc cggccgacga agagctcctg tacctgatcc
    13501 agttcatcgc ggaggaattt cccggtgaaa agacgcccgc ccgtctcgcc gcgaacatgc
    13561 tgcccctgat cacccggctt gcggaatatc aggtcggaaa agagacgatc gcggggtccg
    13621 agcggttcac ctccgagcag tgggatcagc ttgagcaata cctcgagacg gcgaaggcgt
    13681 ttcgccggtg gctttcccgg aaacatttgt ttctggcggc gctcgaagcc tcccggtttg
    13741 acgaaatcct tcccggcgac cgggagatat tcatcggtct gcccgaattg acgccgtcca
    13801 gggagcggtt ttacagaaag atcagaaacg aaagactgtt tatcgatccg ccgctgttcg
    13861 ggcccgaaac ggccgcggga ggccctctgc cgtttgattc cgcgaaaaac cttgttttgt
    13921 cctttggcaa aacggtccgg gcggcccaaa acgcggacat cgaatttctg cgcctggccg
    13981 gacttcatgc agcggcggat tgggtgacgc tggaggtttc ccgctttttg aacgaccgat
    14041 cggaatcgga ccgcctgatg atctgcctgc tggatgagtc gctgaccccg atgctgtgga
    14101 accgttcgct tcgttggttc ggaaatgccg tcaaccttgc cgtctggctg ccgttttccg
    14161 taacgtcggc cggaataagg ctttcagcgg agattcaccg ggccgggcaa accggccgga
    14221 tgccggattt caggaaattt gcggccggcc aggcggcgga attgttcgca aaccggcagc
    14281 gctacgttcg cgaggaaagt gaggcgctgg aagccgccgt atcgatcagc acgctgttgg
    14341 acgactggaa gggaagactg gggcggcatt ttgccgcggc agcgggcaaa tttctcgaga
    14401 cgaaaaaatt ccgcgtcacg ggcggcaggt ccgccccggt gcaggtggtc ggattcggcc
    14461 aggcatcggg ggaaaacttc agcaggggcc tgatacttcc cgtggacagc ggcatcatgc
    14521 cggcgcagcc ctttgaaggc ccctttatca atccggtgca tgttcccttc atgcgcaaaa
    14581 acgtatttga atgccaggat cttcttttcc ggcagattct ggcgcagtgc cgaaaggccg
    14641 ccgtcgcggc ggtggacgac gccatccgcg aacgaacgcc cggctattac atgagccttc
    14701 tttccgagga gtttggaaaa ccgccggtga aggtcgcctt ccgtcggccg aaaccggacc
    14761 cgcgagatga aattcgttgt gccgtcccgg tggacgaggc tctgagagcc aggctcaaag
    14821 cgttcacctt ttcgtattcg agcctctcca aaattctggc ctgccccttt ttattctacc
    14881 atcaatacat cctcggagtg tcttcccctt ccttcatgga cgacgatgaa aaaatcaatc
    14941 tgcagatggg gattttcgtc caccggtttc tccagcagct ctcgaccgtc gggcacgaca
    15001 ggctcgacga ctgggagacg ttttttgacg aactgtggga aagccgcgaa aacacggagc
    15061 tcagaaatac ggaaggcgtg gagatctatc ggctgaacgc gaagctcatt ctgcgggaaa
    15121 tcgatgaaga cgaacgagcg tccggaagaa gggtcctgtt tgccgcgaac gcgatctcgt
    15181 gcgaagaaaa attcaccggc acgctcgccg gtcgctacag catcaccgga cgttcggacc
    15241 ggctggccct gctttccggg cgcgccgagg tgctggattt caaatattcc aaaaagaaaa
    15301 gcggctttgc ccttcccgcg agggaaaccg ttctggaacg atttacggaa aaaggaatcc
    15361 tgcatcccgc ggcgcagttg atcctgtaca gtcattttat tccggacgta aaaggcgccc
    15421 gcttttattt tctcaaggag accgcaaagg accgcatgat ggagcttccg aaggaggcgc
    15481 ttgacgaagc gggcgacctg gcgtccgcca tcggggagag tctcgacagg atcgtgcacg
    15541 gcgcggcgct tgcgccgggg gatgattccc ggcagtgcga attctgccgg cttcagtctc
    15601 tgtgcgggaa agaaggctat taccgggcgg ccggagggag gcgctgatgg aaaacgttct
    15661 gctgaaagcg tcggcaggct ccgggaaaac cacgcaattg acccgggagg tattcgaacg
    15721 gatctccgcg ggagggaaat tcattctggc gctgaccttt acccgcgccg ccaccgccga
    15781 gatgcgctcc cgcatcatgg atcagatcgc gtcaacaaag ggaatgccgc tgctcgaccg
    15841 gatgaggctg atcctggagg cgggccgcgt cggatactcg acgatcgact cgtttttcta
    15901 ccggcttttc gccgccctgg gcgaagcgcc gaagatggcc gacgaaaaag cgcagatcga
    15961 tctgtcccgg gatatcgaaa agcgttttcg cgacggcatc gcgcgaagcg gccgggaaaa
    16021 gcggctgatc atcgcgtccc gtatcctgaa cacggatctg gattcgctct ggggctccct
    16081 cggggaagag cagacgctgg agcgctgcgc ggcggatatg gagaggcttg acgatctgga
    16141 ggacctgatg aaagagaacg tcctcctgct tcgggagctg gagtctctga gccgcgcggc
    16201 aaacggcctt gccgatctgc cggacggact gaaaacccga gtcgtcgcca acctggggga
    16261 ttacgaccgg tttatgtcca gggcgctttg cggctattcc gatctggaac agtcgcgggg
    16321 gctcggcaaa aatattgcgt ggcattcgag cccgtactcg gatctcaacg cgattttcaa
    16381 aaactaccga aagacggccg aaaagcttgc gctaaacaag gccctcttgc gggaactggc
    16441 ccttgcgttt ctttgcgaaa tttatctcga tgcggcaaaa gccgtcaagg aagagaaagg
    16501 gctgattttt tttgccgatg tgcgaaaagc gcttctcgcc ctcgacccaa tcgcgtcgcg
    16561 cgaaaggcca aagctcatgg gcggctactt ctcgcttggc ctggaccgga cggagcacct
    16621 cctggtggat gaattccagg acacctcgac tcgcgacatg gcgattctcc tgccgctcat
    16681 cgacgacatt ttatccggcc ccggcgaacg gggcgaacgg tcgttttttg ccgtgggcga
    16741 ttggaagcag atgatctacg gctggcgcgg cgcgaaccgc gaggcgctgg agacggccat
    16801 cggcggctac cttcaaaacg gcgtcatccg ggagcggtcg ctttcccgca actaccggag
    16861 cacgccgctt ctggtttcat ttttcaaccg gctggtcgaa aatcttttcg agggcaggga
    16921 gaaaacggag acgcaaaagc ccccggagaa aagcggcggg caccgggacg cgctccggtg
    16981 cgtttcggaa gtgagtcttg tcgagctgga aaaggacggc gcgtcggaag cttcctggta
    17041 tccggccatc gccgaatatc ttcagaaaaa aaaggaggag tacggctgtg catgggggga
    17101 gatggccgtc cttgccctca ccaacaacca cgttcagaaa atcgaggcgg agctcgcctc
    17161 gcgaggcatt ggcgtctcct cggtcaaagg ccgccagttg ctggcaacgg aggaaggggt
    17221 tgcggtcatg ctttttctga cgatcgctct ttcccgcgaa ggcgaaaccg ccttcgccgc
    17281 cgcagccgcc cggtcgcccc tgtggaaaga ttggttcgaa gacgcgccgg gaattcggga
    17341 agaattcgcc gctcagtttc cccggccctt cggcctttcc gcggtaagcc tcgccgtcga
    17401 gcgtttgcgc gggaagctgt ccgcgaccgt tctcgatata tggcaggatg aggcgcacgc
    17461 gtttttcagc gaaggcggcg cggatgtcga tgaatttctc gcccgaatgt tcagggttcg
    17521 ctggagcgtg agggttccgg agggcgaaaa ccgggattcg atcaaggtcg atacgattca
    17581 cgggaccaag gggcttcagt ttgcgcatgt tttcattttc tggaacgagg atgagaaatc
    17641 cacaccgttc tatcttcctt cgcagaagtg tcatgtccgg ttttcagcgg ccgagacaaa
    17701 gtgtcttcag gctggagaat cgaccctggc cggggaaatc ctggagaagc gggacgcccg
    17761 cctgatgcaa ctgaggcgcg agagagccaa tgtgttttac gtcgccgcca cgcgggccgt
    17821 tcgaaccctc gcggtttttc tgccggtcag caaaagcggc gaatacaagg cggttcatca
    17881 ggccgttctg aatacgtttg cgcaatttgc gccggaaggg agcaaatcgg agatggtccg
    17941 cttctcccgt ttgccggaag aggcaaaggg gtccgccgtg tgggatgcgc cgtcgcggca
    18001 ctttggcaaa ccggaccccg cgggcgagat cgacccggcg ctgatttccg aatccatcca
    18061 ggccggcatc gcgcggggcg agcggctgca ccgctggctg gccagggttc ttgatcccgg
    18121 gcgcctgcct ccccccgggg agctcggcag ggaggagtat ggcgcggttg tccgttttgt
    18181 ccgcagaaaa gaggtttttg aactcctgtt caggccgggc cgtctctatg ttgaacagcc
    18241 catatcggat caaaaggatt tcggcgtcgt ggacagaatg atcgtttcgg atcatgccgt
    18301 gaccattatg gattacaagt cgggctccct gcgagggctg aggcggaagt acgacgagca
    18361 aagagagcgc tatacccgca ttatgaaatc tctttatccg cagcgaaacg tggagtatca
    18421 tgtcctgccc attgatgaat aggaaacgcg gctgggactt ctccaggcgc ggaagcgccg
    18481 cggaggaagt cacccgcatc attccggccg caaaggggga gaaaagatca tggctgaaag
    18541 gaaaccgtcg cagatatttt tgtcccgggg cggtgtgctg attccgacgt ccggcggaaa
    18601 tctccagctc ggcattccgc cggagaccat caaggacacc atgcggatcg agggcggcgt
    18661 gccccgcacg ttcatcgtgc ccaaagcgat gtttgacgtg cagcacggcg tagcactggc
    18721 cgagatggag tttcccgtct attacaattt ctttttccgg aagagcaaaa ccagaatcgt
    18781 ctgcagcgaa aaacagcgca aaaggatcga aacggtcata gccgaggccc tgttcggacc
    18841 ggcggaggtg gacccggttt tggaatttgc ctgcggggaa tatacgccgg gatttaccga
    18901 tctcaaagcg gaaatgagct atttcaggaa gtccatgatc gagggacgct gtgctgacct
    18961 tggcgacctc atcgaattct gtgtgccgga ccggcgcggg aaagcggcgt ttggagaaac
    19021 ggaggtgcgg ttcgaccggc gcgggaatat ccgcgtttac gatcggggaa agaagattgc
    19081 ctcgtttggc ggggacgctc ccgtgatggt ccgcaagacc cctctggcaa gagtcagcct
    19141 cggctttcag ccgcccatat tcggcgtcac gaccctcggc gccggacacg gcttcgatcc
    19201 cggcgccgac acatcgggcc tgatcctctg ggtgaaccgt cgcgggatta tggtggaccc
    19261 gcccgtcaat tccaccgaaa aactggccgc tctcggcgtc agcccgaagc ggatcgacaa
    19321 aatcattttg acgcactgcc atgccgatca cgatgcggga actcttcaga aaatattcca
    19381 ggaggggaaa attcacctgt acaccacacg caccatttac gggagcttca tgaagaaggc
    19441 cgcggccctg accggcattg aggcgggcct gctcgaacgg ctggtggatt tttatcccgt
    19501 ctgcatcgga aagcccgtga tcatcggcgg gggagccttt catttccatt acaccctcca
    19561 tacgattccg accatcgcca ttcaggcgtc tctgtccgga aaaagcctgg tgtacagttc
    19621 ggatacgatg aacgatccga actttattca acagttgtat gccgacggcg ttttgacgaa
    19681 aaaccgccgt gactttcttc tgaattttcc gtgggaccgg gacctggtgt tccatgaggc
    19741 gggggttccg cccattcaca cgccgatgag ctatctgtgc agtctgccgg aagacgttcg
    19801 caagcggatg tatctggtcc atgtaaatcc ggattccgtt cccgatggct gcggattgag
    19861 gatcgctccc acggggctcg cgaacaccgt ggatctgggc gcttctccga tgccccgtga
    19921 ggaggccgtc gaaatgctgg aggccttctc cggcgttgaa tggttcggcc agatgccgct
    19981 cacgaaggcg agagaatttc tgctggccgc cagtgtgacg caccaccggg cttccgacat
    20041 tattttcagc aagggagatg cgggggaggc cttctgcatt gtcatgagcg gcgagattga
    20101 tattgttttg gaggacagga tcctcacgac gtatgatgcc ggcgggtatt tcggagaaaa
    20161 atgcctgttt tccgatgaag cccggacggc aacggccagg gcgaaaacgg acgtgaagct
    20221 tctttccatt cggaaggagg aaatgctgaa cctgattcgg ggaaccgccg cggaaagcct
    20281 gcttcgtcag attgccgatt ttcagaatgt ggaattgcgc gacgccgtga aagacaacca
    20341 gattctcgat tcgctttcct ccacgcaaaa aacccggctt cacgggctga tccgtccttt
    20401 tcccggcacc ctgcacacag gggacgttat cgcctccaaa gggtcggacg cccgatgcgt
    20461 ctatatgatc cgggagggcc gcgtcgatgt ttaccatcgc cggtcacgca tcgcgacatt
    20521 gtccagagga gacctgttcg gcgtgaaaaa aatcttccgc ccgaaagaga aaaacttttc
    20581 atttgtcgca ggggaaaaga ccgctctgta ttttattgaa caagacgggc tcaggcgcta
    20641 catggaagac aatccgggcg tgtacgtcaa gctgtaccac agcccctatt gaagcggcgg
    20701 gcggcatcgc gaaagcaggc gggccgacgg acgggaaacc gtggtccggc tccgccggaa
    20761 gacattgttg atgtttcagg aatcgaaagg ggaaaggctt catgcaatca tcggacattc
    20821 agcgtttgtt ccacgagcac ggccggaaag tcgttctgca ggagggtgac gtgttgtttt
    20881 gccagggaga ggagagcgac gcggtgtatt ttgtggccgg aggacgtctg ggagtcttcg
    20941 cggcgcagag ctcgtcggaa gatgttcaga tcaacacgat cgaagccgga gagatggtgg
    21001 gtgagctggg agccatcacc cgtcacccgc gtaccgccac gctgaaagcc gcctcgccgc
    21061 gggtggagct tttatccatc gcgacgcccg atttccgggc gctcctgtcc gacagcctgt
    21121 ccctggtcga ggccatgacc ctgaccaacc gggagcattt gatggacgcg gacagggcgc
    21181 gaattcaatt cggaacctcc aaccttcaaa tgcggaagcg cgttgccagt ctggggcagg
    21241 agaaggcgca gctgcaggag ctcctgcagc tgcgcgaaga gctggaagcc atggtggtgc
    21301 atgatttgcg aaatccgctc aacaccgtca tcatggccct cggcctcgtg gagcgcatga
    21361 aggatgaagt gaaagatccc gagaccttcg cgctggccat gcgcctggcg aagggagccg
    21421 cgaaccgcat gaacaggctg attgccacgc tgctggacat cgcccggctg gaggccggga
    21481 aactggcgct gagcatcgcg caggttgatc tgaatacgct ctttgaagaa gtgattgaaa
    21541 tggaaaaggc gggagtgacg aaatccgttg agattaaggc ggctgcggtt ccggggctga
    21601 cggtaaaagc cgaccgggag gtgttgtggc gcgtgatgtc caatctgctg ggcaatgccc
    21661 tgaaattcgc gccggaagcg agcaccatcg tggttgaggc acagcgcgga gatgaccgga
    21721 agattctcat cagcgtgacg gacgcggggc cgggcattcc ggaaggtgag cgcgaaagaa
    21781 tctttgaaaa gtttaccagg gtcaagaacg cggacggcgg agcttacagc gggacgggtc
    21841 tgggcctgac cttttgccga atggccgtgg aagcccacgg cggctgcatc tgggttgagg
    21901 aggggccttc gggcgtcgga agccgcttct gctttcagct tccccaggat taatccgcct
    21961 gccccgggcg gccgggttac cggacggtga tctccaccct tcgatttctg ggctccggcg
    22021 tgttgtccgg cgtgggaatg agcagattcc tttcgccgtg ggactcgatc gtcaggtcat
    22081 gcacttgaat gcccgcgtcg cggatgattt gcgccacggt cctcgcccgc tgcagagaga
    22141 tctgttcatt gtaatctctg tttcccatgg tgtcggtatg gccgatgacg gatacgtccg
    22201 gcgcgggata tttcattgcc gcctccacga tggacgggat cagggccttt gagtcgtttg
    22261 tcagcagcgc tccgcccgct tcgaagtaca gcatgaaact gaccggcagc ggcggctggg
    22321 cggccagcgc cacggcgaag tctttctgaa tcttctgttc atccaccaaa aagggctttt
    22381 ccgatttgcc gtccagcgag gcggcggttc ggggctggtt cagaagcgtt tccttttttt
    22441 caccgctgac cgccagcttt cccgtcgttc cgtcctgatt ttccatcaga acgacataag
    22501 acttgggtga acagccggcc agcgccagcc acacggcaag aagcaccaga cctgcctttg
    22561 caaaagcctt catgcgatgg tcctcctgat gttacggctc cactttgacc agaaagcggg
    22621 ttcctcggca tccgatcacg gcggccggcg tcttcatttg gacggcctcc ggcttgagtt
    22681 tggcgatcac gcccgaaata acctgcaggg tgcctttcgt catgctggtg ccgaatttca
    22741 gccggtcttt ccccggttca tagaggtatt catccaccgt cagctccgtg tccggcccga
    22801 acgacattac agtattgtcc ctgaaagtga tgcccagaga tccgtccggt ccggttttca
    22861 ataggttgcc catctgaatc ggcgtgccga cccgggcgct gacggccttt ccggcgtcga
    22921 tcacttccgc tttgcctgaa gcggttttga cgtagccgat ggtgtccgcg gcccaggcgg
    22981 gcgtcagagt ccaaaacaat gcaataaaga ctgccgcaaa tcgcttcatg gtatttcctc
    23041 cttggcaaca ggttcggtta cttttcttcc agaatgatcg tatcaccctt tttccccgag
    23101 cgcaaccggt cccggtggag acggaccagt tcgtcggccg gtctttccgc ggccagccgc
    23161 tcgaacgctt caagggcgag cggctcccgc cttttgagga gttcaaaggc cttctcgtaa
    23221 gccgtgtccc gttccggctt tccgccgttt gcctctggcg ccgcgagggg ttcatagacg
    23281 ctgatgggct gtgttttgcc tttcagcacc agggaggctg cgggccggac gacggcttcg
    23341 ggacagcctt gaagcgtcgc ttccgacacg cagatcagcg tgcccaggta tttgttcgcg
    23401 ccttccaggc gggaggccgt attgacggcg tctcccagcg cccggtagtc aaacagggtt
    23461 ttgccgccga aatttccaac aatgacctcg cctgagtgga cgccgatgcg ggtctgtccc
    23521 aactcgatgc cttttgcccg gcagtccgcc gcgtagccga aggcaaaggc atgcatttcc
    23581 agtgcgcacg acagggcccg ggcgcggtgg tccggctgcg cgaccggcgc cgaaaacatg
    23641 atcgcgacgg aatctcccac gatccggtcc agtgtcccgc cccggccaaa ggcgatggtc
    23701 accatcgcgt ccagataggc gttgagaacc gaaaccgcgt ccgcggggtc catcttttcc
    23761 atcaggcctg taaacccggc aaggtcggtg aaaacaaaac tgcattcctg ccgccgccct
    23821 cccagctcga gctggtcggg gtggcggaca agataatcga cgcggttggg ggaaatgtag
    23881 cgcgaaaaag cctcgcgcac ccaccggtgg cgtctttcgc tggtccggtg gtggatgatg
    23941 ctgcccagaa tgaacgcggc cagcagcgcc agacccggcg ttacggggtc cagaagcagg
    24001 tggtatcgga caaaagcaat ccacgcgccc catccggcgg cgaaaagaat cgcggcggtg
    24061 atgcaggccg agacgagcgc gggagccgaa agcgcaatga ttccgactgc gagccctccg
    24121 gccagaatgg tcaggccctc gatggccgcc gcccaggaag gccttgaaat atacgttttt
    24181 gtcaaaatct gttccagggc ctgggcgtgc gcttccacgc cgggcatcac cgttcccatc
    24241 ggactgaacc ggatgtccat gagcccctgc gccgaggcgc cgaccagcag aatatggcct
    24301 tcgagctttt cccgcggcgc ttctcccgcc agcaccttcc atgccggaat gaaccgatcc
    24361 gggacggatt ttgtatagtg cacccagatt tctccccggg gcgtggcggg aaccgtaagg
    24421 gagccgatcc ggatttcctg aatgccggcg cccttttccg gcagtgtcct gacgatataa
    24481 ttgtgcgcgc cctgaaccac cctcagggat tccgcggcaa gggacgggaa ggcctgctcc
    24541 tgaattttaa tgatcagggg cacccggcgt acaacgccgt ccgaatccgg gataaagttc
    24601 atggcgccgt ttcccgcagc cgctttttcc agcgggggga tcgacgttac cgcgccggaa
    24661 aaggaataca aatggggcag cggggattct ccggaaaaaa caaagcgaaa cggccttgcc
    24721 gggccatgat ccctggcaag gccttgttcg gcggcaaagc ctagaacgac gcgcccttcg
    24781 gccagtgtat cggccaggat tgcatcatgg ccgggcatgg cctccaggcg gcggcggatt
    24841 tcacggggca attcccagtc gccggtgatc cgctccggag acgtccggtc cggttcggca
    24901 aacatgatgt ccagggccac gacgcctgcg cctgcgtccc gcagcttttc aatcagccgg
    24961 gccatccgcg tgcggggcca gggccactga ccgagtcgct gaaggctttc ctcgtcgatg
    25021 tcgatgatgc gaacgggggc ggcttgatag ggacggggat gccagcgctg gtactgatcg
    25081 aaaacagcgt tgcgcagcac ctgcagcggc agcggttccg cgaggaaaac ggccagaccc
    25141 aaaaaaacgg ctgaaaaggg cgccagcagg ccgggggata atttgggaaa aaacctcatg
    25201 atttcaccgt ccggtttgat caaaaggata cgacaggcgg caggatatgt caattggcgc
    25261 atcatccgac aatgtgtcga taaatcttga caattatttg gcaatccatt agctttccag
    25321 tttgtttttg agaattgtgg caggtgtgcg gattcattct tccggacagc cgccgcgttg
    25381 ccttttttag aaggggggaa gatcatgatc ggtgtgctta ttacaaccca cgggaatctg
    25441 ggcaatgagc tgattcacgc cgcggaatcc atcaaaggtc cgctcaaaga cgtgatgcat
    25501 gtctgcatcg aacagacgaa gggcctggaa gatatgaaga aagaaatctc caatgccctc
    25561 aagaaactgg atcacggcaa aggcgtcctg attctgaccg atctcttcgg aggcacgcct
    25621 tcgaacattt ctctgtcttt catgaaagaa ggcaaggtgg aggtggtgac gggcgtcaat
    25681 ctgcccatgc tgctgaaact gtccgatgtc aaggaggaaa ccactctcaa agagtttgcc
    25741 ggtttcatca aggattacgg caagaaaaac atctctctgg ccagtgaaat tttgagcaaa
    25801 aaagcaatcg ggtgatccct tggcggaatc gtacgacatc gcgctggtgc gggtggacaa
    25861 caggctggtt cacggtcaga ttctggaagc ctgggtgccg tttgtcgaag ccaggtgcat
    25921 catggtggtg gacgacaatt ccgccagcga tttcttctgc gagacggtga tccgcatggc
    25981 cgtccccagc gacattgagg tcaatatctg ctccgtggat gattttgccg ccaattacgc
    26041 gtatgtccgg ggaagcggca aaaagaccat cgtcctgttt ggcaggattg ccgatgcctt
    26101 gaaggcctac gagcagggat ttcattttga agcgctcaac atcggcaata tctatcacga
    26161 agattacaaa gtctgctgcg cgccgtccgt gtttctgtgc gagccggaaa ttgcggacat
    26221 tatcaggttg cagcgggaaa agggcgtggc cgtcgaggtg aaaagggttc cccgggagaa
    26281 ggccgtggat atacaggacg ccctcaaagc gtcctgcgat tcgaaaccgt agcgggggga
    26341 tcgcgtgctg acacaaatcg tcatcctgtc cctggtcgga gggctgctgt gcctggaccg
    26401 gatcttcctt caggccatgc tgtcgcggcc gatcatcatc gctccggtca tggggcttct
    26461 tctcggcgat gcctacaccg gcctcatcat cggagcgatt ttcgagctgt tctggatgga
    26521 ccgcgccccc atcggcattt acattccccc caacgactcc attgcctccg cgttcgccgc
    26581 cgccctggcg attctggccg gccagtccct gggcgccgtc acgaaagaac tgacggctct
    26641 ctcggttctg ctggctgttc ctttcggtat tctggcaaaa aaaatggatg tccgaatgat
    26701 ggccgcaaac gaccttcttt ccgaccgggc gctggaagcg gcccgttcac gggatcttcg
    26761 cgcgatagaa aacatgacct gcctcgcgct gggccgcgcg tttgttcttt atgccggcct
    26821 gctgtttatt ctccaggtga ttttcattcc cctcgtgatt aatttgtacc cgaagcttcc
    26881 ggtgcaggca ggcggcgcgc tgacccttac ctactatttt ctgccggttc tcgggaccgc
    26941 cgttgcgctc agcaccatca agctgcgcgg agcggttccc gttttttgcg cgatcttcct
    27001 ggcggccgtc ctggccatgg actattttca tgtcttctga caagaccgga cagctcttca
    27061 gcgttgatct gatgaaagag gaagatctgc cggagatcct cgcgatcgag gccgcgtcgt
    27121 ttgtgacgcc ctggaccagg ggcatgtttc aagacgagct tcgcctgccc catgcccagt
    27181 gcctggtgat ccgggcgcgg caggcggaga aaacgcaggt tgccgcctac atcattttct
    27241 ggctggtggc cgacgaggtt catttgcata atatcgccgt ccgcggcgag tttcgcagga
    27301 aggggctggc gtccggcctg atgaacctca tgcgcgacat cgcggggcag gcgggggcgg
    27361 accgtcagac gctggaggtc cgggaatcca acaccggggc aataaacctt taccgccggt
    27421 gcggttttgt agtaaaaggg aagcgccccc gttactatga tgacacgcgg gaagacgcgc
    27481 tggtgatgtg ggcggaggtc cggcagaatg gccatcatga ataaacagat caccatcggc
    27541 agcgtttccg gaaatgacga aatccggccc ggctatgttt tgatgacgat tcatgtgcac
    27601 cggtcgtttc ccgaaccccg gcccggacag ttcgttatga tccgcatcgc cggtttgcag
    27661 gagccctttc tgtcccgtcc tctcagcatc ttctcgtttt cgcgcggccg atcgcatggc
    27721 cttgtaaagc tgctgtaccg cgtggccggc aagggaacac agattctcgc cgggctgatt
    27781 cgtggatcgc aggtggaaat agtagggccg ctgggcaaag gctacaccgt ggatccatca
    27841 cgggaaaacc gtgtgctgat tgccgggggc atcggcgtgg cgcctctgtc gcttttggcg
    27901 cagcatctct gccgactgtc gggccgcggc gcaaatcccg ctgtcgtgta tctgggcgcg
    27961 aagacggcgg atgagctcgt gggccttgat cagcttcaca aatattgcaa cagcattcgg
    28021 gtctgcacgg acgacggctc tttgggcgag aaatcccttg ttacgaggac gtttcagaaa
    28081 gacatgaaaa aacacgatcc ggcaaggacg tccatctatg cctgcgggcc gaagccgatg
    28141 cttcagtcgc tggcgggcat tctgaaagat cgctacttct gtcaggtgtc gctggaagaa
    28201 cgcatggcct gcggtgtcgg cgcctgcatg ggctgcgccg tggccgtgag agatgaaaat
    28261 ggagacaaga cttaccggcg cgtatgcgcg gacgggccgg tctttgatct tcagaaagtg
    28321 atttgggaat agttttttgc cttttgcaat ggaaacactc attggttttc ccatgaacca
    28381 gcattcgggg gtcgtcagtt gaaacgtacg cctacaacag atgcagcaat ggttgtggaa
    28441 atcgccggcc tcaaattgaa aaatccggtc atgacggcct ccggcacctt cggctgcgga
    28501 gaagagtatg ccggttatgt ggatttgaac cggctgggcg gcatcgtcgt gaaaggtttg
    28561 tcgctgaaac cccgcctggg caatcctccg ccccgcatca tggaaaccgc cggcgggatg
    28621 ctcaacgccg tgggactgca gaatatcggc gtggatgcgt tcatcgagga aaagcttccc
    28681 tttttgcgaa cgtatgacgt ggccgtcatc gccaacatct acggcgagag ctgcgcggag
    28741 tatgcgcagg ttgcccgaaa actcagcgcc gcgccgggtg ttcacgcgct ggaagtcaat
    28801 gtttcctgcc ccaatgtcaa aaaaggcggc ctgagcttcg gcgcagaccc gaaagcggcg
    28861 gcggaagtga caaagcgcgt caaggccgaa acgcatctgc ccgtgatcgt gaaactcacc
    28921 cccaacgtta cggatattac ggttattgcg agggctgtgg aagaagcggg cgcggatgcc
    28981 gtctccctta tcaacacact gaccggcatg tccattgatc ttaaaaccag aacccctcat
    29041 ttgaaaaaca ttaccggagg cttgtcgggc ccggccatca agcccgtggc gcttcgcatg
    29101 gtctggcagg cggcgcaaaa ggtgacgatt ccggtcatcg gcatgggcgg catcatgacg
    29161 gccgaagacg ctctggaatt tatggtggcc ggagccgccg ccgtgcaggt cggcacagcc
    29221 aatttcatca atcccctcgc cacaagtgaa atcgtgcaag ggatgaggca atatgtcaaa
    29281 catcacaaac tgaaaaacat tacggaaatc gtcggaacgt tgaaagttta atttgccgcg
    29341 acaacctggg gcatgtggag cagccaatgc ggtttgaaaa caccgttgat tgcagggcgg
    29401 gtctatagta atcttcaggt tatcggattc gctaaacatc gacttcatag gcgaatgtag
    29461 ggcgggtctt cagacccgcc gagccattgc gccgttctca tttggcgcgc ctgaaggcac
    29521 gccctacgtt tagtggggcg ccgtcaaaac caggggaaaa tggaaatagg caatgcgcag
    29581 gggaactgaa gaaatcctgt ctggtgttta tctgatcggc gggccgaatg taacgcaggc
    29641 ggacgatgcg gccgtttatc tgatcgattt taaaggcgag ctggtgttga tcgacgcggg
    29701 ggcgggcgga agtgcttcgc agctcgtccg caatattgaa atgctggggc tgaatcctgc
    29761 gctggtctct catgtgattc tgacgcactg ccacatcgat cacatcggcg ctgcgccatt
    29821 cttccgcgaa aaatacaaag cgaaaatcgt cattcacgag atggatgcaa aagcgctgga
    29881 aacgggcgat tccgtccgaa ccgcggccaa ctggtacggc acgacatttc cgccgacgaa
    29941 gatcgaccgg aaattaaccg gcgcacggga gacgttgaaa ttcgacaatg atgaactgca
    30001 ctgtctgcac acccccggac atacgcccgg ttccattgcc gtctatctgg accggtcggg
    30061 ccaacgggtc ttattcggtc aggatattca cggtccgttc cataaagcct ttggttcgaa
    30121 tattgaggcc tggaagaaat ccatgcaaac cctgcttgcc cttgaagccg acatcctgtg
    30181 cgaaggccat ttcggtattt ttcggcctaa aaataaagca agagcctata tcgaaagata
    30241 tcttgaagag tacgagtaag tactgtgcga ttgacgcttt ggcgaaattc aaaggtgggt
    30301 gagaaaatga gaaagacact gtttgttttc ttcatgctgt tttgggctgc aggcattacc
    30361 ggtgcggcac aagctggaat atttgacgat attctgaaaa agcttccggc ggccaagagc
    30421 gccgcacctt cagccggtct ggatcagaaa acgacgattt ccgggttaaa ggaagctctg
    30481 gtcatcagct caaagaatgc cgtcaaagcc gtgtcaaaaa aagacggcta tttcggcaat
    30541 caagtggtga aaatactact tcccgataat attcagaaag ctgccgatgt ggttgccaaa
    30601 ctgggctatc agaaacaggt tgatcatttg attctcagca tgaatcgcgc agcggaggcg
    30661 gctgccccta aggccattcc tctttttgcg gatgccatca agtccatgtc catcgatgat
    30721 gcaagaaaga ttcttcaggg gggtgatgcg gccgccaccg agtatttcaa agaaaagacg
    30781 tcggcaaaac tctatgagga gttcaagcct gtggtcgtgg caaatatgaa caaagtcggg
    30841 gtggcgaaag cctacaagaa tatgacggct ccctatgaag cgcttcccct tgtatccagc
    30901 aagtcaatcg atcttgatcg ctatgtgacc agcaaagccc tggacggcct gtttctgatc
    30961 atcgcagaag aagagaagaa gatacgcacc gatcccgcgg cgagagtgac ggatcttctc
    31021 aagactgttt tcggcaaatg agggacagca cccgtttatt gacggcgccc caaaaggagc
    31081 gaagaatgga gagccacttt gcaaggctgt catggatccg tcaatttccg tgatgagttc
    31141 cctttaacgg atatatcctg aacagcatcg ccgacaagaa tcaaaaggat cctttccggt
    31201 tcatcgaagg ttgcttttga cgcttcctaa tttccttttt ctcttaaaac caaagaaggc
    31261 acgtagctcg ccaggcgacg cggaaatact ctggtcaaaa cagatgcggc aaaccagttt
    31321 tgaatgccgg gaatgtagat gggtttcccc tttttgaaag cctttactcc cttgcgggcg
    31381 atattctccg gggaactgag gccgctgata gaataccacc agagtttttt tggaaatcct
    31441 ttcccgcgaa gcatcggcgt atccgtatag gagggattaa gggtgcagat tttgatgccg
    31501 gaagatttta tttcctggcc taccgcctca ctcaggctct gcacaaatgc cttggtggcg
    31561 ccgtaaacgg catggtgcgg tgtgggttga aaggccgagg cggaaacgac gttaaggatt
    31621 ctgccggatt ttttctctat cagcccgggt aaaaataacc gcatcagttt aaggtaggca
    31681 atggcgttta ccgtgatgac ctcaagctgt ccatccaggg gtagatttgc aaaatctccg
    31741 tagagcatga ttccggcgtt gttgaccaga atgtcaatag tcttgccggt ttgttcaacc
    31801 tgtctataaa gcagttccgg tcccgaagcc agggagaggt caacggggaa tttttttgtt
    31861 ttgatatgat attgatgttc cagatagtcg gcccaacgac aaagctgatc ctcttcacgg
    31921 ggatgggaac cgagaaagag attggatcct tcacgggcaa aacactctgc aagagccttt
    31981 ccaatgccgg aggcggcacc ggtaatcagg acgtttttac ctttgagtat cagcattttg
    32041 cctctttcca ttagccgaat cgtaagcccc ttttttctca tcgactggcg actcgtcgtg
    32101 caaggggagc aggcgggttt agagggataa ccccgcctgc tcatgggaaa acagcgtatg
    32161 caaccttgtc gggggcttac attttttcca ggatgtgaat gcacatggcc gcttcttcaa
    32221 cgccgatgtt gccgccgccg ttttccgcca gaccaatctt tgccccttca acctgcctct
    32281 tccctgcctc gccgcggagc tgcataacaa gctcatggat ctgggagagt cccgaggcgc
    32341 caatgggatg ccctttgcat tcgagccctc cgctggtgtt gatgggtttt ttgccgccca
    32401 gcgttgtcgc tcccgattcg gcgaaaggtc ctccctcgcc catgggacag aaacccatgg
    32461 actccgtttg atgcagttcg ccccaggatg tggcatcgtg gagttctgcc agatcgatat
    32521 cctcgggtcc cactccagcc atatggtatg cctgctttgc cagacgctcg ccgatgtccg
    32581 gagcgttcaa atcgcgatcg gcgccggtgc ccaggacgga agcccggatt tgcacgggcc
    32641 gggcagacgt catttttttc aggaattttt cggaacaaat gatcgtggca gcggacccgt
    32701 ccccgaccgg tgcacacatg gcgcgcgtca ggggataggc caccatcttg tcggccagaa
    32761 cctcttccgc cgtcatgact tgctgatact gagaaagggg attgagggat ccgtgcaagt
    32821 ggtttttcgc gcaaatcacg gcaagctgct gctgtgttga gttgaagcga ctcatatgcc
    32881 agcgagcccc catggcgtat gcatccatga agatgctcct tccttccccg ggcgccgatt
    32941 gaccggcagg gattttcacg gtgaaggttt tgctgatttc catcagattt tggatatgct
    33001 gctcgaaatt gcccacgtcc atgcaggttc cgtaagcgct cagtgaaagc attttgttcg
    33061 ggttggtgat tttttccgaa ccgattgcca tagccacgtc atacatccct gaacggattc
    33121 ccgtgtacgc aaggtgcagc gcggtagaac ctccggcaca ggcgttttca acattgacga
    33181 ccggcacctt gccaacaccc atgcgccgca tgacaacctc accccggatg gaatgctgat
    33241 tgtcgaacat tccccagaaa gtgttagaga agaagacggc atcgaggtct tccctcgcaa
    33301 gccctgcatc cttaagcgcc aagtccgtgg cctcatgggc catagaaatt acggttttgt
    33361 cgggatattt gttgaaccgg atcatcccca caccgatgat atagacatta cccatttgca
    33421 tattcctcct tgagcagcgc agtaccgatt ccgattgcca gagaatcgtt ggagccatct
    33481 tcgatcattg aggcccttgc atcgcgaaag agtttttcga tgggatattc cttgctgacg
    33541 ccgttgccgc cgaataactg cacggcgtcg cttgctacct caaaagcggc attcgtacag
    33601 aaaacctttg cggcgaccga gtagggaaca gcaggcgggg tggtgttcat attgtaaatc
    33661 atcgccgctc tggataattg tctggccgcc tgaaccttcg aaaacatgtc gaagattttc
    33721 ttttgcaccc attggtgatt ggagatggtt ttaccgccct gtaccctttc tttgcagtac
    33781 tgcacggcca aatcgtaggc ggcctgggcg acgccggtaa aaaaggcgcc catgcaggca
    33841 ttggctgttg ccagcgtgat gtccgtcatc atctcgtagc tttcggggtc tataatcatg
    33901 tcttcgccgg ggcagatggc gtcgtcgaag aatatctctc cctgaggaag ttctctctgc
    33961 cccattttgt ccaaaggctt tcctctggta acccccggct gtgtcaggtc tatgaggcaa
    34021 atgccgcctc cgctcatgcc ctgggcgctg tctatgttga cgaagagggc ggcattgctc
    34081 gcaatgggac cattggagac ccaggccgct ttctgaccgt tgatgatcca gtccccgttt
    34141 ttctttttgg ctttgacctg ctgggtgatc ttgggatcat gaaagaaagg cgtgcccggc
    34201 atgagaatat cggaaccgtg ttccggttca gtgatcgacc agcagctcat gatgctggcg
    34261 tcttcgcatt cgacaaaggg gataatgaat ttatttacca gatgatcatc aggcaccatg
    34321 caggcgtaaa aggccgaaaa aatgctgacg cccaaagata cggtgaagcc ggcgcttcca
    34381 taggccagtt cttcaaaaaa gatatgcacg gacaggggat ccaggcccat gccgccgtac
    34441 gcatcggaaa tcagggcggt atggtattta ttcttgtaca tttgccgcat gcacttccaa
    34501 tagagagaat ccttcgcgat cacttcctcc ggcgacagct tgtcaagctc aatggacgcc
    34561 ggccgcagca cttccatgga aaacttgtgt acggattctt tcagggcaat atgctcgtct
    34621 gttaagtcca ggtttaaatc tctataagcc atggttttct cctttaatga tcataaagct
    34681 tcatggggtt cattgcgtcc gcaatttcgt tggcatgata atgcggctat ttgaaatctt
    34741 cgtatttgta gatgtcgcct tcctttttct cgaacatctc cctcaatacc ctcgtcagcg
    34801 ccttttcggt gatcgtcttc ggtatctctt tcaccacgtg caggtacgac gggatgaaat
    34861 tgccctgcag ctcgtttttg catttctgat agacggaagc aggatcgatg acgctgtctt
    34921 caaaaggagc aatggccgcc accagatcgc tttcgcccgg ggagccggaa gcggccggga
    34981 tgccgtaaac gcaggcttcg ctcacatccg ggtgccgtcc aatgatggac tccacgaaat
    35041 cgggctggat gaaatcgcca aagcgtcgca gctccgtacc cttcctgaag tcgaagaagt
    35101 accatccgtt ttcgtccttg tggaccatat cgcccgatct cagccatcct cccttggtct
    35161 tgtcctgtga ctcctccggc agtcccaggt aatccacttt cgtatcgccc cggatcatac
    35221 gggaaatgag ttcaccggtc tttcctgccg gaaccgggtt gtcatgatcg tcgacgacct
    35281 tgaattccat tacgccgggg accggcttgc cgaacgatcc gacaggaccc tggcctgtgg
    35341 gtttgtaggc aaaccccccc tccactgcgc cgtaccactc gagaatctgg acgttgaagc
    35401 gcttttcaaa gccttcccaa atggccatgg gcgttccggc actgataaca accttgacgg
    35461 gattgtctcc gtcatcgtgt ttttccggct cgttatagat gcccgccgcc atccctccca
    35521 ggagagagaa ggacgtgcat ccatacttgc ggcagatgtc ccagattctg cttttggtga
    35581 atctcgggct gaaaacggcc ttgaccccca gactgagggc gggaaaaaga gttacggcct
    35641 gggcgtttcc atgggtgagc gaaagaccat tatagaggat gtcgtcggat ttgtatttcc
    35701 agacgatact gttcaggatg ttaaacagcc ccgtgcgatt gttcctgatc atgaccccct
    35761 tgggatctcc ggttgttccg gaagtgtaaa taatctgcat gggatgacgc acatccatga
    35821 tctgctgctc caccggtttc caggtgtcct tttccatcac ctcgttcaag gcatgatgct
    35881 ttgcggaaac ggctgcattc tgccccggct gataggcgac ggtgatgaac cgcagggtcg
    35941 gcacatcggt gatgaccgcc tccaggtctt ccagacattc accggaaaca atgaccgccc
    36001 gggccttgca gttgttaagg aggaacttaa gccgctgtcg cttgttgcgg ggatccacgg
    36061 gaacggcaat ggcgcccaga gtgatccccg ccatcattcc gcagacgaac tccggatgat
    36121 ttctcatgta gatggcgaac gtatcgcctt tgccgattcc tttgtccata aaaagccggg
    36181 ctattttatt ggaacgctca tgaaggatgc gataggtgag gatgtcttca ccgtgttcgc
    36241 cgttttcaaa aatgtagatc tccttgtccg gcatttcatc cgcccgaatt tcgatcaaat
    36301 gggaagcgat aacctgatta agatgtgctt ttgatgccat gatttatttt cccctgctaa
    36361 attttgaatg tgccttcgtc ttccacggcg cctatgaatc ccctcgtgaa ggtgggaaat
    36421 tctttctgga aggtttcatc ccagtgttct tttgtccaat acttgtgccg gtcaaaataa
    36481 ggatcgctct tcgcggcgct taccgacaca ctgacgatca aatccacatc ctcaatcatc
    36541 ttcagggcga aggcgcccat ccccagggtg ctgaagacgc ggcagtgaag tcccaatttc
    36601 caggccatgg aggccgccgc gttggtcgcg atgttgatat tgatgttttt aaactgacaa
    36661 ctggggcccc atgccgtcag cgattccact acttcggcct tgttcagttc cgcgcaggtt
    36721 ttataaccgc aggcgccgca gttgtagttg aaatcagatt tccttttatc ccccaggaga
    36781 aggagtccgc agggctcctt gatcagctcc atgacaaaac gaccgtcacg agcggctaaa
    36841 ggtgacatat cggccaggga ataacaaaac tcgccgatgg aagcgatctc ctccttcccc
    36901 agggatacga tctttaccgt attcttgttg ttaaacctga agctgttgtg agcggcgacg
    36961 gtcatcaatt ctatggacct gtccaagccg ctttgcacca gatcattcat gtctcgaatt
    37021 gccatttttg cctcctgcca tttgttgata tcattagcta tagatggatc tgaactgcgg
    37081 ccacagcctc ttgatgatcc ggtcattcat agtgtgttcg ttgatctccc cgtacttcag
    37141 cgggatgtcc cggaagggac tcttttccgt gatggagacg gctaccgcca aagcgaatcc
    37201 agtggcatga ggcaaaatct tcatcctcat ggcggcggcc ccgacagacc agaaaacgcc
    37261 atagtcgatc cccagatttc ttgcctggga aatgatgccg tcgatggcat aggaaatatt
    37321 gttggcccgg aagatacaaa ggggaccttt gaaagcgaca tcggcgtcat cgggtagcct
    37381 ttccgtttct ctcagcgatt cacaggtcat cttgccgcac atattgcagt tgatatcggc
    37441 cggatcgttg acacagcgaa gggatgtaac caccagcacc gcatcggcgt cccgcagcat
    37501 ggcggcatct cttttgaaaa aatcccaagc cttgttttca cgggacatgc tttccatgcc
    37561 cagacaaagg tcttctatgg cgcatggatc atcgatgata tggatcgtac attcaccgac
    37621 gcctcccaca cggggtgtcg ttgttccgga agccagcaga agccgcgagg caattcttac
    37681 cgcttccagc ctgtcttgct cgaattgttt catgctcttt tctatgctca tacccccccc
    37741 ttgcttgagt gaaacgtttt tatagaccgt actttcggga gatttccggg taggcctcgt
    37801 aggccggttt caaaagtgga agcagcttca atactttggg aagaggccct ttagccttga
    37861 tcaaaccctt agccagggcg atgggcatcg atacttcctg cagccagaac tgatggcagg
    37921 tatctccgct cagggtcatg tcaatcgtcg gtttaagatc ggcatctccg cagataacgc
    37981 ctttttctgg cgtcagccag atggttccgc cgggatcgtt gatggtaaat ttgatggtga
    38041 tgttggactc gatgaatttc ggtccgacag aagggtggtt catgagcgtg ttaaaaaggg
    38101 tgccgagaac ttcatacatt ttttcggtat ttgcgaatac tgccataata cttccctcct
    38161 tcatctgtct tttattggtg gttaagtaaa aacaatcgtt cgcttcatcc gcctctgaaa
    38221 caactacccc tccttgattt atttttcagt tatggacgca caatcaaaga tctgtgatct
    38281 gttgtgcaat agcctttgct gatagacaaa acaaaaaaac aatggaacta aaattcttga
    38341 gatccggcaa tgaacttgct gaagaaattt ataatccgtt taacgtgaga gggaagggag
    38401 atccagagat ccgaaggaga taggtgttgt cttagaccgt ggaggaaatc agccctgagg
    38461 tcagagcaat atcgtgtgaa aaatggcaga caggaactat tttgggcgtt ctgaatcacg
    38521 atattctccc tggcaaatat tatggcaaac attgagccgc gaagttatat tttgttcaga
    38581 ctgccggtta ataaaccgtt aattgcatcc gtgaatgatg ttattgtcca ctgcgttgaa
    38641 tgactgtccc ggtcaagata ccagcgaacg gctaatccat ggtatacggc tgatatggcc
    38701 tttgctgttg catcgggatc cgaacaattg aaggtgccat cattattgcc ttcaatgatg
    38761 gcatctttga ggagagccac ccaattattt acccattcgt ggaatattgc cctgtagctt
    38821 tctttgtggc atgcctcagc catgaaatcg aagagcagcg ggtagccggt cgaaacatcg
    38881 ggatctcccc aattatagag ccagccaaag gaaagcaatt tggagagagg atctgaatat
    38941 aaatctatgt tatgtttgct tctctgaaaa atgaaatcaa aaaatgattg aaaaacggct
    39001 tcgcagatat catccttgga tttaaagtaa tgggtgacgc ctccctttga aagccccgct
    39061 gctttagcaa tggactcgac cgtgatatta ttcattcctt tggccgaaat ttcccgtaaa
    39121 gctgcgtcaa caatctgagc ccgtctgagg acttctcgtt gtgcattgga agacataaaa
    39181 cctccttcct tgctaaacca aaaaaccgac cagtcggact aaaccaaact gatttagcga
    39241 atatgccatg caaaatatat agtcaatact actttatgtt cattttttat aaaaaggtgc
    39301 tgtaccgtca agggggtcga tcgacgtctg tcaagctgca atccgggtca tgacggaaaa
    39361 gatcgctaca taagcgccag gctgtccgcc acggccatgc cggccaaggt cggttgaagg
    39421 cggccgtctt tcagctccag aagaccgttt tccatcagtt catcgatgac ttgcttcttg
    39481 cccacaagaa ggtccgcgcc aaagcgattt ttatattgct ggagatggat tccgtcatta
    39541 gtgcgcatac tcaggaagag cgcctcaagg gcgagctgct ccgcagagag ggattctgat
    39601 ttgtccacag gctttttccc tccgccgact tcgttcagat aagccttcac gtctgccttg
    39661 ttccaccagc gcctggaatc gaggaaagaa tgcgcggccg gccccaaacc cagataaggc
    39721 gcgtgccgcc agtatttcat gttgtgtctg gattttaagg aatcggagcg ggcgaagttg
    39781 gatacctcgt aatgaattaa accggcatcc gtcaggattt tcgatgtgtt cataaagaaa
    39841 tccagtgctt cattttccgc gggcagttgc aggctttctt tactatattg tttatacaga
    39901 ggcgttttct cctccaggga gagctgatag caggaaagat gctccggtgc aaatgacagg
    39961 gcttgctcca gggttttgag ccaaaggcga atatcctggc cggaaacgcc gtagatcaga
    40021 tcgaggccga tattttgaaa tccggcgttt ctggcttcgt cgatcgctgc cagggcttcc
    40081 tttgcggaat gccttcgtcc aaggaacttc aaaatccggt cgtcaaagga ctgcacgccg
    40141 atgttcaggc ggttgacacc cgcagctttc aggtcccgaa gatagttctt cgaaacgtct
    40201 cccgggttga cttccatggt gatttcaata ttttccggat accggcactt gtgcccttca
    40261 gggtattcgc cggtatgacg aagcgggtgg ttgcttcccg ggtcatcaaa acggaaatga
    40321 tgccgggcgg ccgtgagaag atcgtcaagc tgctttgcgg agagcaggga aggggttccg
    40381 ccgccgagat agatcgtgtc aaaaccttta aagggaacaa acgatggctt gtagaaagcc
    40441 atttcccgca cgacggcctt tacaaaatca gggatcagat caggagaaac aacggagtaa
    40501 aagctgcaat atccgcattt gctcaggcaa aacggaacat gaatgtaaag cccggcttgc
    40561 tcattcttaa tcattgtccg acttcagcac cgccagaaat gcgctctgcg gaatgctcac
    40621 cgagccgatc atcttcatgc gcttctttcc ggctttttgc ttttccaaca gcttcctttt
    40681 gcgggtgatg tcgcctccgt agcatttggc cgtaacgtct ttgcggaagg cgctgatcgt
    40741 gctgcgtgcg atgatttctc cgccgatggc gccctgaatg gcgatcttgt acatctgccg
    40801 cgggatttcg tctttgaggc ggtcgcaggc caaaagggcg cgggcgcggg cgcggtcacg
    40861 atgaacaatt tgcgacagtg cgtccacctt ttcgccgttg accagaatat ccatcagcac
    40921 cagactgctt tcacggtaat cgatcatgtt gtaatcaaaa gagccatagc cctgcgtgac
    40981 agatttaaag cggtcgtaaa agtcgaagat gacctcggcc agcggcattt cgtaggaaag
    41041 ctccacgcgg ccggggccgg tgtaattcat gctggaattg acgccgcgct tttccatgca
    41101 cagcttcatc acggcgccca ggtagcgctc cggcatcatc atggaagcgc ggatgtacgg
    41161 ctcctcaccc ttgtcgattt ccgccgggtc ggggtagtgc gtcggattat cgacgtagag
    41221 cacgcggccg tcctcgaggg tgaagcggta gcggacgctg gggacggaaa gaataataga
    41281 cagatcatat tcccgttcca gtctctcctg cacgatgtcc aggtgcaaaa gccccagaaa
    41341 gccgcagcgg aatccctggc ccagcgctgc ggaggagtcc ttttcgtaaa tgaaggacgc
    41401 gtcgttgagc ttgtattttt ccagcgagtc ggccaggtcc tggtaatcgt ccgacgcgat
    41461 gggataaatc gacgcgaaga ccacgggctt gacttccttg aagcccggca gcggctgcgg
    41521 gcagggcctt tcctgatgcg tgatggtgtc gcccgtgcga acgtcggaga cggtcttgac
    41581 gccggcgatg atgtagccca cctcgccggc ggacagtttc tctcgtttgg cgcgctgaag
    41641 aaggaaatgc ccgacttcct ccaccttgta ggttgagttg ttgtacatga agcggatgat
    41701 gtcgccggct ttgacgccgc cttcaaaaac gcggcagtga atgatggttc cccggtaggg
    41761 gtcgtatttc gcgtcgaaga tgagcgccgc gagcggatta tccccgctgc ccgtgggcgg
    41821 tggaatgcgt tccacgaccg cttccaggat ttcctcgatg ccggtgcctt ccttggcgga
    41881 agccttgatg tgcgtgtccg ggtccagcc
//
LOCUS       DAOPAF010000010        37063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1275_length_37063_cov_57.287046, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000010 DAOPAF010000000
VERSION     DAOPAF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 37063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37063
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_37063_cov_57.287046"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..850
                     /locus_tag="PLQ77_02175"
     CDS             155..850
                     /locus_tag="PLQ77_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPL09518.1"
                     /translation="MLASDDTQSDAWRRDLIQDYLTRDIPSLGFALPGPVLSRFWKMI
                     AHYHGGIFNASKIGQSMDISHNTVRKYLDILEQTFMVRLLQPLEINLKKRLVKSPKIY
                     LRDSGLLHTLLEIDSATELYGHPVFGASWEGWCIEQIIATLPFWQPFFYRTSSGEEID
                     LVLVKGKKRLIFEIKASLTPHLSKGFADTMKALTPERVWVVCPMTDSGYPIQSDGRVT
                     GISECLGDLAVYE"
     gene            complement(976..7311)
                     /locus_tag="PLQ77_02180"
     CDS             complement(976..7311)
                     /locus_tag="PLQ77_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09519.1"
                     /translation="MTGLLSKRWVNRWAIAVLGAAMLLAFCAAFALALSPPVGTVIPN
                     TATARFTGANGYAFPDVPSNRVETAIGGAPVLAITAVATPDPTAPGGTLTYMITVSNT
                     GNLPASAVTVAATLSPILQFQGASNGGISSPPLVTWNLGAIGPGGGVTFTLTAVVAAG
                     TLPGTVIPFTASTTSAEGAANSATLTTTVGAGSNLVFEKSGAPETVAPNGVITYALSY
                     RNIGNQTANGVRIVDSIPSGTAYVAGSASAFGSLTGSVISWNIGDVAAGGAGEVSFRV
                     RVSPVAVSGQQISNTASILSTAQTKTSNTVVTNVSTPSLLLLKLDTPDPVRAGNNIVY
                     TLQVENTGSIPLTGIVLSDPIPVGTTFVSAGNGGVAAAGARQVEWAIGALAAGAKQTV
                     TLTVQVDKALVQGQVIENTATASSNEMPPRTVRAVSVVSARTQGTIEFFDAAWQPAYA
                     YRSGNAVNLQVLDADQNLNPLVAETVTVVLTDSKTGDTETVIMTETGPDTGIFRGGVT
                     SSLAASAANSGILTVSANSRVTATYSDALDAVVVSTASALIDPLGIVFNSVTGAPVAG
                     TVVTLRNWNGVANSCDLTSWPVLPPGEVNPAVPTGADGKFAFPLVPPGDFCFQVTPSA
                     GYTFPSAVAASDLPAGFTIGNGSRGEKFTLNVGDPPLIRDIPVDPPTGRLTIAKTASK
                     TTAAIGDMLTYVLNLTNNGSSPVNSLTITDIMPHGLAYIAQSTKTNGVASADPQTAGK
                     RTFAWTIASLAPAASVEIAYRVLVGPDSQAGTGINTATAAGLSLGKTVVSNTASVKVK
                     ITEGVFTSKGTIIGRVFLDLDENRLAGKDSGIPDVALYLEDGTRVVTDKRGRFSISGV
                     SAGTHVLRLDETTLPAGAKLIPLNNRFMRDGASQFVDMTPGGLFKANFAVQKGSMILK
                     APAPEAQKEKQEQENTEQQGRTLVEALSDIFSSVPASRKAVSSKASPAGAATVEGPPV
                     KKPEEMKASVSTRVASDPQEEAKASSAMPDAAATKEAPAVKTSAEPKTDQTPPAESRP
                     AAKEENEADAGAEKVSAEKEAGLEERIKTFSPDLEFLNLRDQEVAARKNIRVVVKAPL
                     DTELTLTVNGDKVDNRQIGKQIKYAPGKVAIYEFINIPLKTGEENLITAEVRDPFGNV
                     RSSQTIRIKAAGDAARMVIRPERTEAPADGQSKISVNVSLEDREGQIATFSGSAAVSL
                     SAGEILEKDADPLADGHQILIRNGVAQFTVVSPRETGEAKIEIQVNDVSESVKIYFLP
                     HLRPMLMVGVGEVVLGNGRSSGDLDYIKSRSWFNDGAYVNGRGAFFMKGNVFKDFLLT
                     ASYDSNKNKTDELFRESDTRLDSEEKYPIYGDESKTGYEALSREKLYVKLEKGKSYLL
                     YGDYRTDLTETTLAAYTRSFNGLSAEVNTDRFRLRSFGTHTDQSQFVDTLPGKGISGY
                     YYLNNNTIIEGSERVVIETRDRLQPDRVLNREVKSNGSDYDIDYAMGTILFKAPVPSH
                     DADGNPVYIVATYESRQEGSKYYIYGGRGAFKVTDWLQVGATGVIEENAISNYQLFGG
                     DIALKLPGKTLVKAEYVNTRGLFDINNTYAAKDGEGWSVNVESQPLNRLSVNGFYRNM
                     SDYFSNPSATDAVRGTQKFGFDAAYEIQPSLTLRAKYLDEQDRINNSTHRLASVGATK
                     KFVKTKISAELTHETSDNLTVTPAQTPFTPGGLLNGVPFLNAYETPDQATFARVELER
                     ELLTDLSLTLSHKQDLGGNSYFVSQGGLNYKINEFNRLYLREEYAKYQQGTQTRTLIG
                     AESSLVKNTTAYSEFRLADGSAGYRNQQVIGLKNKFQIIRGLNANIAGEYLSTLSGQK
                     NPNEPDAYAAAASMDYLAKEDLKITGRAEHRHEIVENGTDSYLAEVAAAYKLHPDFSL
                     LVRERYFIEKKGGDQNDTNRLLLGLAYRPLENDRFNALGKVEYKYEKRGTAQPAYGTD
                     SFIASAEGVYQLTQTMQVMGKYAGKLEKDEDFSSYTDLMAARIMIDLTDRFDFGAEYR
                     LLTSHKTNTRQHGGSVEFGCRIVDQIWLSLGYSFDRFDEDLAGDSYHGEGPYLKVRFK
                     FDEKTLRKLNPLADKNSSSAPQAAASIEKALSSYLSK"
     gene            complement(7406..9130)
                     /locus_tag="PLQ77_02185"
     CDS             complement(7406..9130)
                     /locus_tag="PLQ77_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09520.1"
                     /translation="MKTVKAPLKFVVLLIAGLLLSTMLSAGTAFAAGTPAGTTITNKA
                     TATYTDANNNTYAPVESNTVSVTVSQVAGVTTSPATVSQNGSPGGKTYFSGQIVNSGN
                     GTDTFNLTAAGLPAGYTYAIYKDTNGDGILQPSEEVAGNMLGASVSLDADQVQYAIAV
                     ITAPAGAAAGSTASLNFTATSVLTPAVSSTSTMTVTIQAAVITFSKSATPAAPKPGET
                     VTYSITWNNSGTAPGYLVVLTDAIPAETTYKTGSMTYSGAARTDAADADNADYNVTNP
                     GKVTAAIGTVAAGGSGTFSFQVTVNANIAASTVINNTASATYRTNAGDPGTETTVNTN
                     TSPFTVSQAAGVQVMPPTLTTDELVGNQNLHPFTIKNTGNSTDIYSISSVGLYWTWAV
                     YNDVNQDGKYTAGVDTPVTDSNADGKIDTGAVAPDETKFYVAVVTVAGSNGQQGKHTI
                     TATSVYDTTVTGTSIKFTNIQTPVMGLVKSVSPAGPQPPGTQLTYTITVTNTGAAPAQ
                     TFVVTDVLSSYLNYVPNSITVAGAGQTDAADGDFGHYDAVSGTVMISIPSIAAGATIP
                     IVYRATIK"
     gene            complement(9179..9697)
                     /locus_tag="PLQ77_02190"
     CDS             complement(9179..9697)
                     /locus_tag="PLQ77_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09521.1"
                     /translation="MTNVKTFRWMVLWGALFLAALLLAAPQARADEPRIALKVSGEKE
                     VTQIRNGKKTVTTAPLDKIKSGDILVYTVMYHNTGKSVARDVTVVDPVPANTVYLPGS
                     AAGKDAKIFFSINGGATFVEPPVLRKSLDAAGKEILQPAPPELFTHIKWIIKAPVPAN
                     ATGTVTFKVKVK"
     gene            complement(9795..12779)
                     /locus_tag="PLQ77_02195"
     CDS             complement(9795..12779)
                     /locus_tag="PLQ77_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPL09522.1"
                     /translation="MARRMAFYLLTVCLFMAVGSEPLHAAYVKRYTTIANGGMTYTGN
                     TLGLSKATNSNQPGTNGAIGAFITTDTSLKVGNFPAGTTLNWTKNSSSAILRLPAGSS
                     VIYAELVWGGSYRYGGQDVSGSLNSAVTFSGPAGSMSVSPSPTTAAVTGSGEYYYVRS
                     ADVTSLVQAGGAGTYTVGGVPATVAATENNANAAGWTLAVIYGNASLPARNMTIFVGA
                     ELTSSSVSTTSSVSGFCTPGAGTINARMMVSAIEGDSGITGDQMRFGPTAGTLAAVSG
                     PNNPSSNFFASQINQDSGARDTAGTFGDRNSTPGSQGNGVRAGWDITNVDVSARMQSN
                     QNTAFARGTTSSDRYTITTIGLQINVGAPVFPSAVLSVDKATTYIGDTLTYTAILNNS
                     AGSADALDVVFTHSLAGGTAFISGSFKVNGVAQSGANPVSGVNIGTVAAGATVTVSYQ
                     VQVTSIPLPPAAATYAGQSSWAYQYQSCAGFPLNNGTNTTNISTTGTVRLQPTKSANP
                     SGKVLPGGTVTYTIAIPNTGTANSSGTTFADPIPAGTTYVPGSTTLNGGVVADVGGAM
                     PFATARQVNSPGGASGQIKIGETATVSFRVTINANPPLIITNVATIDPDGAGPAPSIT
                     VPLTNPPVQADLAVAITDNQTTAVAGSPVSYIVTVTNNGPDGVISLNLDATIPATILN
                     PTRSPSSGSYNAATGEWTGLSLAAGASVTMTIAGTISPAATGTLTFPITVSPSPGVQD
                     TNTANNTASDTDTLTYQADLAITKTDGKTSVTPGSVITYTITVTNNGPSRVESLTVID
                     TLPPLLQSPVFKPSQGIYNEATGLWTGLNLMPGQSIALSLTVLVDPAVAGSFTNTATV
                     SPPAGVTDPVPGNNSADDTDTTMPHITLTKNVDPSTATPGQDVLFTIYYRNVGGSAAS
                     NLIIFDTVPPNTTYVTGSLKSGNAASTYATANSLTDIADADVGQINGENVTFIISTVA
                     GDDGADNAGNDEGKVYFKVRIR"
     gene            complement(12902..13327)
                     /locus_tag="PLQ77_02200"
     CDS             complement(12902..13327)
                     /locus_tag="PLQ77_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPL09523.1"
                     /translation="MLKKAQKTAQREAIMSYLKDNRNHPSVADIYAAVSEQIGTISLT
                     TVYNTLMLLQKEGLLKEIPVHGSSGKRFDPNSSPHDHLICTSCGLIVDIDQTSAINIF
                     PEHLQGFEIQEISVKVYGLCPQCHNKAKEGQGSLCLKDK"
     gene            13756..15006
                     /locus_tag="PLQ77_02205"
     CDS             13756..15006
                     /locus_tag="PLQ77_02205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL09524.1"
                     /translation="MFDYLLSKEQLKIRDEAREFVKSIPRQTILDMDADKIKFPKEFL
                     SEAGKRNLLGCRYPKKWGGRDLDWVTTCMLMEEIGTLGYEMACVFGVGAELVCDAIIL
                     HGTDEQKEKYVKPLLRGDLFAAECLTEPRGGSDFFGATTKAEDMGDYFLINGQKRFIV
                     GGEGADFFLLYAKTDFDPKTKPQKSITAFIVDRGPGVETKYLYGLMGCRGGGTARLVF
                     KDAKVPKANVVGKVNGAYDVFNTMMIPERLGTAAMTIGAARPALEVATNYTTRRKAFG
                     QVIAQFEGVSFQVAEAIMLLDAARSMAYVTARAVDGGVNMNRIRRMVSESKKFITESC
                     QKVVHNSMQVCGGIGYTNILPIERIHRDVRLASIWTGTSEVMSMITAHEWYREYLMAK
                     AAGLTRDCELDAAEAEAADEIIYE"
     gene            complement(15173..16702)
                     /gene="guaA"
                     /locus_tag="PLQ77_02210"
     CDS             complement(15173..16702)
                     /gene="guaA"
                     /locus_tag="PLQ77_02210"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPL09525.1"
                     /translation="MILIIDFGSQYNQLIARRVRENQVYCRIEPPELPLSAIRAMAPE
                     GIILSGGPASIYAAGAPRAQSGIFELGIPVLGICYGLQFMVDALGGRVIGSAKREYGF
                     AELTIKKQKGLFSGVKPKTTCWMSHGDSIGELPRGFSVTASTANTKVAAAEDARRRFY
                     GLQFHPEVVHTREGGKMLANFLFGVCNCARTWSMRSFVAEAVEDIRRQVGKEKVILGL
                     SGGVDSSVAAALLHRAIGKQLTCVFVDNGVLREGEAKRVIDMFKKHLRINLRFVRAGR
                     IFLQRLKGVTDPERKRKIIGRTFIEVFDAEARKLKDVRFLAQGTLYPDLIESRSAFGG
                     PSAVIKSHHNVGGLPKTMKLKLVEPLRHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTVPRLSLLKRADRILHQEIRDANLYGKLWQSFTILLPLKSVGIMGDQRTY
                     ENIVAIRAVTSDDAMTADWARLPHDLLARISTRIINEVRGINRVVYDISSKPPGTIEW
                     E"
     gene            complement(16721..18892)
                     /locus_tag="PLQ77_02215"
     CDS             complement(16721..18892)
                     /locus_tag="PLQ77_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPL09526.1"
                     /translation="MKKMQPTLAAERQYAVEKEKFVPVSQYFGEDTFSHAVIKEKLPK
                     DVFKKLMQAMTENKSLDEETANVVAHAMKEWALEKGATHFAHWFQPMTGITAEKHDAF
                     ADPCGPGAVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTVFISYTGQVLDKKTPLLRSLRALNQSALRMLKLLGAKSVKKVQVNLGIEQ
                     EYFLIDMDYYYKRQDLVLAGRTVVGAPPPKGQELEDQYFGSIKERISSFMHDVEEELY
                     KLGIPAKTRHNEVAPSQYEIAPVFEEANLATDHNQMIMETLKHVAKKHRLSALLHEKP
                     FAKINGSGKHVNWSLSDDRGNNLLNPGKTPQDNISFLVFLIAAVRAVYRHGDFLRATV
                     ASCGNDHRLGANEAPPAILSVFLGEQLTQILDNIEKGIVHKATNAEIIDLGISSLSQV
                     SKDYTDRNRTSPFAFTGNKFEFRAVGSSQSVACPAYAINTIVAESLDELAEKIKAKGS
                     KNINQAVFDVLKNEIAQLRPILFNGDNYSKEWAAQAKKLGLPNEKTTPGALKALVTDR
                     ALRLFEKYRVLSNEELRARYLIHTERYIKDLEIEVNCLINICLTQVIPAAAAYQQKLA
                     RAILDAKEVLGSAAVISSQAELLKKILDLINDIYAACREITAGMQAAKAMDGEQKKAE
                     ALCARVKPKMDELREYVDALENIVDDEIWPLPKFWEMLFIC"
     gene            complement(18995..21598)
                     /locus_tag="PLQ77_02220"
     CDS             complement(18995..21598)
                     /locus_tag="PLQ77_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPL09527.1"
                     /translation="MIPYARVSSGISGLDKILNYLQMGDNVVWQVDHIEDYKKFVDPY
                     VEQALADRQRLVYMRFANHPALLEARPGIKICQLNANSGFESFSTEVHNIIRDEGRDV
                     FYVFDCLSDLLQAWATDLMIGNFFVITCPYLFELNTVAYFAILRSRHSFKTVARIRET
                     TQVLLDICNREGRLCVHPLKAWKRYTPTMFLPHIMEDQACVPILNSADAASFLSCLTD
                     RNATGPERNLDYWDRIFLKAGSLLENPDAAPEKQDMVEILSRVMIGREQRILSLVREY
                     FDLEDLVEIKKRLIGTGFIGGKSVGMLLARNILRKDPSFDWNAVLEIHDSFYVGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFDVARELKNKMLHGVFPDEIKEQFQLMLEYFGQAPI
                     IVRSSSLLEDAFGNAFAGKYESDFCVNQGTPEERYRHFEEAVRKIFASTMNEDALTYR
                     LQRGMAQQDEQMALLVQRVSGSHSDRYFFPGLAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPTVKPYTKPEDIKRFSQHEADVLNLRDNEFQTLQT
                     AELLREDLVAHLNLIVEEDKEAADYLRSVGRDAKQAWIITFEELLSATPFVKTMSKML
                     KTLEQVYRYPVDIEFTVNFDAQGKTRINLLQCRPFQTKGHYSRVELPEKISRSRMLIR
                     QDANFMGGSVYQAISRVIYIDPRAYSGLPLTEKHEIARLTGKLNRRIGKREAMPTVLL
                     GPGRWGTTTPAMGVPVTFSEINNMTAIGEIAYQDGALIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKEGVVFNVQWIKKQPNILADLMPDDARFAGVVRVCDVRAKDVRLMSDIVTQK
                     MICFLGDACSP"
     gene            21930..23162
                     /locus_tag="PLQ77_02225"
     CDS             21930..23162
                     /locus_tag="PLQ77_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPL09528.1"
                     /translation="MNSGDTAWVLTAGALVLLMTIPGLAFFYGGLVRRKNVLSILMQC
                     FIIVCVISLQWVLFGYSLAFGPDFHGIIGTLEWAGLAGVGAAPNSNYAATIPHSVFMI
                     FQAMFAIITPALIIGAYAERVKFPAFLLFTLLWATFVYDPLAHWVWGAGGWLKSLGGL
                     DFAGGIVVHVSSGISALVLALLLGKRVGYNHKPIRPHNLPFTVLGAALLWFGWFGFNA
                     GSALAADGLAANAFVTTNTATAAAGLTWALIEWWHNGTPTILGVATGAVAGLVAVTPA
                     CGFVNPMNAIFIGIIVALVCYTAVAVIKGKLGYDDSLDAFGVHGVGGTVGTIATGLFA
                     EKAVNAAGADGLLFGNLHQFGVQCLMLVVTIAFAAIMTFIIFKIVDAAIGMRVEENNE
                     IIGLDLTQQSEAAYTVIE"
     gene            23175..23516
                     /locus_tag="PLQ77_02230"
     CDS             23175..23516
                     /locus_tag="PLQ77_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPL09529.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVDVNLMTVTNVVGQGRQKGVTE
                     VYRGAKEAGGLLNKVRLDIAVNEAFVEPTIDAVTRGAHTGSVGDGKIFVVELAECVRI
                     SSGERGGVAIG"
     gene            23563..24438
                     /locus_tag="PLQ77_02235"
     CDS             23563..24438
                     /locus_tag="PLQ77_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09530.1"
                     /translation="MKKNIKRIMMIALLALMLTSMPMAAGAGEEPQSAGPKQQEDKLT
                     GEIAASVLSAYIWRGQEMTRDSVAIEPSFTANYKGFTAVLWGNLDTRPYAAGDGKHSS
                     TWSETDFILSYAHEFGIVKAGAGYIYYGLAGMTPLGVDLLDAQELFVSLGLNTILAPT
                     LTVYKEIDHYHQWYATLGVSHTFALHERVGLKLAAQASYLKSEDETTYPKFDSNSAAT
                     TDNFNNFHDGVATISLPVAVTGNLTITPTASCVFPLSEDAKYEMRARGLKGTANPSDR
                     DSYYVYGGVTLSLTF"
     gene            24548..25240
                     /locus_tag="PLQ77_02240"
     CDS             24548..25240
                     /locus_tag="PLQ77_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPL09531.1"
                     /translation="MIARYFKHAPFEDIGSIRSWLTTAGYTITCTPFFQSANLPALEE
                     IDLLIVMGGPMSVNDEKRYPWLVPEKQFIRRMIISGKPVLGICLGAQLIAGAMGAKVY
                     PNGVKEIGWFPVHGVSSGGRSVFRFPQSVQAFHWHGETFDLPAGATRIAKSDGCENQA
                     FQLGQSVIGLQFHLETTPQSAQDLVAHCRDELIGAPYIQTETEILSAKPAQYQSINRL
                     MDKILNFLARTA"
     gene            complement(25241..25624)
                     /locus_tag="PLQ77_02245"
     CDS             complement(25241..25624)
                     /locus_tag="PLQ77_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPL09532.1"
                     /translation="MNQLKKIEAIIREDKVNDVIDALKEIGIVGMNTFEVRGHGRQGG
                     VQLVGRAGTYQVNMLPKIQINIVLNTRNLDAALEAILCSAYTGETGDGIIFVTPVEDV
                     IRVRTRERGPGAMMYPGDIDERRSK"
     gene            25851..25924
                     /locus_tag="PLQ77_02250"
     tRNA            25851..25924
                     /locus_tag="PLQ77_02250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25883..25885,aa:Gln,seq:ctg)
     gene            25947..26294
                     /locus_tag="PLQ77_02255"
     CDS             25947..26294
                     /locus_tag="PLQ77_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPL09533.1"
                     /translation="MLMKLIIAIVQSDKLKDVKQALRDVNVGKITVSTVVGCGAQVGK
                     TDTRDGVITNGDLLDRVRFEIAVNDDYVKPTLEAVIRGARTGSIGDGKIFIVPLEECV
                     RIRTGETGRLAIG"
     gene            26486..28024
                     /locus_tag="PLQ77_02260"
     CDS             26486..28024
                     /locus_tag="PLQ77_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPL09534.1"
                     /translation="MSIHSYEELAALHAISKILAQPMDLRDQLEKILHEMSLRLGMQR
                     GMISLLDHEEKEAWLDIAHDVNIDGMEVTYKPGEGITGQVAETGRPMAVANLGQETHF
                     LDRTGARRHLNRAELSFLCVPIIYDERVVGVLSADKVAKQVEDLEKELAMMGSVAELM
                     AKAVHIRALEEENRRLRKIVGQQHAPSTDIIGHSKAIQEVFGLVAQVSDSNTTVLITG
                     ETGTGKELIARAIHNNSPRKSGPLVQVNCAAIPDTLIESELFGHEKGAFTGALQTRRG
                     RFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRVVNVNVRIIAATNRIL
                     EQDILADRFRADLFYRLNVFPVYLPPLRERGSDVILLADHFLLKYSKEMGKNVKKIST
                     AAIEAFLSHKWPGNVRELENCIERAVLLTKTDTVDCLHLPPSLHAGERSPERRDHSKL
                     SSVVEAQERALIIDALRETGGNQTKAAKILGTTKRVIQYKISKMGIDPGKFRKNTRDA
                     ALKP"
     gene            28246..29376
                     /locus_tag="PLQ77_02265"
     CDS             28246..29376
                     /locus_tag="PLQ77_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HPL09535.1"
                     /translation="MCRLFAVTSSQPQSPVMAINALNVMKEGHDGSGVGLFLTDLGGE
                     FEKFKNEPILSGIFSNEGIKALDHFMIDQDFMVKYKLSLRPTKPAPSGTPKRDNYVIR
                     VYEYPAEWENLSREEIEYRLMMVQLQLRQIGEKDQSMLIFSFWPDVIMIKEVGDPLAV
                     AEYLGLDRRDLTARVILCQGRQNTNYSINIYACHPFFIQGMATMTNGENTAFVPIREF
                     LMSRNFPGYTGYQSDSEVFTHILHYTQNKLGLGMEMYKHVITPLRDEELARHPDGRML
                     RNLKQSCRPLIIDGPNCVIGCLPDKSMFMVQDAKKLRPGVVGGRPGIFAFSSEMCGLD
                     AAIPERDINLDDQPMRYETVIVRRGRQEIEKWNQWDTLPHLH"
     gene            29340..30974
                     /locus_tag="PLQ77_02270"
     CDS             29340..30974
                     /locus_tag="PLQ77_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPL09536.1"
                     /translation="MEPVGHIAPSTLSAKDLPWQIAWQKDRCTLCGRCTTVCPVRAIE
                     LGVHRKRALTTPVGLESRPANLFSVYHGIDQRTDPAHACVGCGMCTLVCPNNAIVPLR
                     ADETDKLRFHVNQGGEARRRGGRRNAPESVLDQIKFVRISMLTDPALDAGRHEFELRT
                     LLGRILPPERMLQAGRENGWMPPVREIYPMIIGGMSFGALSPNMWEGLQMGVAYLNEE
                     MGMPVRICTGEGGCPPRLLKSRFLKYVILQIASGYFGWDEIIHNLPQMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNRLIAAIRGVPQGISLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTLAVLNNLTRNPYAAGLAIDGEDGGTGAAYNVSMNHMGHP
                     IASNLRDAYLTLVKLGKQNELPLFAGGGIGKNGNLAANAAALIMLGASGVQTGKYIMQ
                     AAAGCLGSEGDRCNVCNVGVCPKGITSQDPRLDRRLDPERVAQRLVDVYLTFDTELKK
                     IVAPLGRSTSLPIGMSDALGISDADAAGRLNIKYVL"
     gene            31038..33374
                     /locus_tag="PLQ77_02275"
     CDS             31038..33374
                     /locus_tag="PLQ77_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL09537.1"
                     /translation="MTRKDTIKIAGVENTVRVDSRILEERIQKAVADGCRHIEVTALG
                     QHGIGGRLWRAHDEPVKVDVLGTSGQRLGSMGFANTTIQAFGPASDDVGWLNAGAKII
                     VHGNATNGAANAMAQGRVYIGGNIGARGMTMTKYNPRFEPPELWVFGKAGDSFAEFMA
                     GGIAVICGAGAEEEKNVLGYRPCVGMVGGKIFFRGSQEAYSQPDARLVTDLPDDEWEW
                     LKQNLKTFLDAIGRQYLFEQLAEKREDWQLLIARKPSEKASRFVRPMAKFKQEVWEHE
                     LGRGGLIGDLSSLDRSPIDVITTGALRRFVPSWENEKYLPPCQAACPTGIPVQKRWEL
                     IRKGHLEEAVNLALEYTPFPAAVCGYLCPNLCMQNCTRSLVHLEPVDVTLLGKASLEA
                     KEPRPRPQTGKKVAVVGGGAAGLSVAWQLWLKGHETVIIESQKRLGGKIADAIPQSRI
                     PSDVVEHEIGRLAKSIHKVRLGKPLTKEQFARFKSEHDFVVIATGASRPRVLNIPGVE
                     KSVSALEFLRRSKLDCAKVGEKVVIIGAGNVGCDAASEAFRLGAKSVILIDVQEPASF
                     GAERKQAEAAGAKFLWPRFTRAITDQGIELTGGEMLPAETVIVAVGDLPDLSFLPQSI
                     ATEKGFVVVDKTYATSDPQVYAIGDAVRPGLLTDAIGAGRIAARSIDDRLRGAAETYD
                     KLPPIGQERVKLQYFDPRIRAFADTASCATNCASCGACRDCGLCEEICPQNAIFRKTL
                     PDGGFEYAVDGESCIGCGFCAGACPTGVWELSENKPLE"
     gene            33404..35572
                     /locus_tag="PLQ77_02280"
     CDS             33404..35572
                     /locus_tag="PLQ77_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPL09538.1"
                     /translation="MKKTKATAAAERHYVIEKEKFVPVSQYFGEDTFNHKVMKQKLPK
                     DTFRKLMEAMNEDKKLDHETANIVAHAMKEWALEKGATHFAHWFQPMTGITAEKHDAF
                     ADPCGPGAVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTAFISYTGEALDKKTPLLRSSRAVSQSAVNMLKLLGAKNVRRVYSNLGPEQ
                     EYFLIDMDYYYKRQDLVLGGRTVVGAPPAKGQELEDQYFGSIRERISSYMHDVEEDLF
                     KLGIPAKTRHNEVAPSQYEIAPVFEEANLAVDHNQLVMDTLKSVARKHYLSALLHEKP
                     FARINGSGKHVNWSLADDNGNNLLNPGKTPQDNISFLVFLIAAVRAVYRHADILRASV
                     ASYANDHRLGANEAPPAIISVFLGEQLTQILDSIEKGKVAKATNEEIIDLGISSLPQV
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSISFPAAVINTIVAESLDDLAEKIRAKGS
                     KNINQAVFEVLKSEIRVIKPILFNGDNYTKAWEAEAGKRSLPNFKTTPAALGALVTDK
                     ALRLFEKYRVLTRVELQSRYIIYLEKYIKDLEIEVKCLGNICLNQVIPAAADYQKRVA
                     EAVIATKAALGGAAVAAEADLLKKVIRLVNTIYAGHKEIMAGMEAANAGHDEAKKAET
                     LCEKIKPRMDALRESVDELENLVDDERWPLPKFWEMLFIC"
     gene            35650..>37063
                     /locus_tag="PLQ77_02285"
     CDS             35650..>37063
                     /locus_tag="PLQ77_02285"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPL09539.1"
                     /translation="MKNTKFIFVTGGVLSSLGKGLAAASIAAVLESRGLRVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSSTCMGKRNNLTTGQVYFSVIT
                     KERRGDYLGKTVQVIPHITNEIKDFIRETAKGFDVSIVEIGGTVGDIESLPFLEAIRQ
                     FRNEVGRQNAIFIHLTWVPFIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLS
                     DEIKGKIALFCNVEVNCVFTAKDVGCIYECPLVYHREGLDAKIIELLNMWTGQPKLTA
                     WENVVEKFYHPAHEVCIGIAGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFVDTEKI
                     EQEGAANHLSNVDAVLVPGGFGNRGIEGMILAARYARENQIPYFGICLGMQMAVIEYA
                     RDVCGLTGANSSEFDPATPHPVIDLLPEQRTVRDKGASMRLGAWPCSIAESSFAFDAY
                     GQSEISERHRHRYEFNNDFKK"
BASE COUNT         7830 a        10320 c        10779 g         8134 t
ORIGIN      
        1 gctcatgtcg gaagacttga aagaagccat gatgtctttt ctggaaagac gcccggcgga
       61 tttcaaggga aaatgatctt gatgagccct tcgggtttcg gccaaccggc ttttgagccc
      121 gaagggctgt cttgagatca tcagtccatc gtctttgctt gcatccgatg acacccaaag
      181 cgacgcctgg cgaagggatt tgattcagga ttatttaacc cgcgacattc cgtcgctggg
      241 atttgcgctt cccggtcctg ttctgtcgcg cttttggaaa atgatcgcgc actatcatgg
      301 cggaatcttt aacgcatcga aaatcggcca atcgatggac atctcgcaca acacagtccg
      361 taaatacctt gatattcttg aacaaacgtt tatggtgcgc cttctgcaac ctctggagat
      421 caatcttaaa aaacgcctgg tcaaatcgcc taagatatat ctccgcgaca gcgggctttt
      481 gcatacactt ctggagattg acagcgccac ggaactttac ggccatccgg tattcggggc
      541 atcctgggag ggctggtgta ttgaacaaat cattgcgacc ctgccgttct ggcaaccgtt
      601 tttttacaga acgtcttccg gtgaagaaat tgatctggtt ttagtcaaag ggaaaaagcg
      661 cctgatcttt gaaatcaaag cctctctgac gccccatttg tccaaaggct ttgccgatac
      721 gatgaaagcg ctcacgcccg agcgcgtctg ggttgtctgc cccatgactg attccggcta
      781 cccgatccaa agcgacggac gcgtaacggg aatcagcgag tgcctcggtg atctggctgt
      841 ctatgaataa tcggcgagag ttatgcccag aaatcatagg gagaacaagg ggttgaaacc
      901 ccttgttcca gcttgcaggg aacggtcgcg accgttccct gctttatttc cgtaaacggg
      961 cgacgccgaa attcatcatt tgctcagata agatgaaagc gctttttcaa tcgatgcggc
     1021 ggcctgcggc gccgaagaag aattcttgtc ggccagcggg ttcagctttc gcagcgtttt
     1081 ttcatcaaac ttgaagcgga ccttcaggta cggaccttcg ccgtggtaac tgtcgcccgc
     1141 cagatcctcg tcaaaccgat cgaaagagta acccagggaa agccagatct gatcgacgat
     1201 gcggcagccg aattcgaccg aaccgccgtg ctggcgggtg ttcgtcttgt ggctggtgag
     1261 caggcggtac tccgcgccga aatcaaaccg atcggtgagg tcgatcataa ttcgggctgc
     1321 catcaggtct gtataggaag aaaagtcttc gtctttttcg agttttcccg catatttgcc
     1381 catcacctgc atggtttgcg tcagctgata aacgccttcc gcggaggcga tgaaggaatc
     1441 cgttccatac gcgggctggg cggttccccg tttttcgtat ttatactcca ccttgcccag
     1501 ggcgttgaag cggtcatttt caagagggcg atacgccagg cccagaagga gccgattggt
     1561 atcgttctga tcgccgccct tcttctcgat aaaatatctt tcccgcacca gcaaagagaa
     1621 atccggatgc agcttgtagg ccgcggccac ttccgcgaga tacgaatccg ttccgttttc
     1681 cacgatttca tgccggtgtt cggcgcggcc tgtgattttc aggtcttcct tggccaggta
     1741 gtccatgctg gccgcagccg cgtaggcatc cggttcgttc ggattcttct gaccgctgag
     1801 ggtgctcaga tactccccgg cgatgttggc gttgagcccc cggataatct ggaatttgtt
     1861 cttcaatccg atcacctgct ggttgcggta tccggctgat ccgtcggcca ggcggaattc
     1921 cgagtaggcc gtcgtgttct tgacgagcga cgattccgcg ccgatcagcg tgcgggtctg
     1981 ggttccctgc tggtatttcg cgtactcctc cctcagataa agccggttga actcgttgat
     2041 cttgtaattc aaaccgccct ggctgacgaa ataggaattg cctcccagat cctgcttgtg
     2101 actcagtgtc agggacaaat cggtcaaaag ctccctttcc agttccaccc tggcaaaggt
     2161 cgcctggtcc ggcgtttcgt aagcgttgag gaagggaaca ccgttgagca gcccgcccgg
     2221 ggtaaaaggt gtctgggccg gtgtgacggt aaggttatcg gatgtttcat gggtcagctc
     2281 ggcgctgatt ttggttttga cgaatttttt cgtcgcgccg accgacgcca gccggtgcgt
     2341 cgagttgttg atcctgtcct gttcgtccag gtacttggcc cgcagcgtca gggacggctg
     2401 gatttcatag gcggcgtcga agccgaactt ctgcgtgccg cgcaccgcgt ccgtcgccga
     2461 gggattgctg aagtagtcgc tcatattgcg gtaaaaaccg ttgaccgaca ggcgattcag
     2521 gggttggctc tccacgttca ccgaccagcc ttcgccgtct ttggccgcat acgtgttgtt
     2581 gatgtcgaac agcccgcgag tattgacata ttccgccttg accagcgttt tgccgggcag
     2641 cttcaaggcg atatcgccgc cgaagagctg ataattgctg atcgcgtttt cctcgatgac
     2701 acccgtggcc cccacctgca gccagtccgt cactttaaaa gcgccgcggc cgccgtagat
     2761 gtaatacttg ctgccctcct gccggctctc gtaggtggcc acaatgtaaa cggggttgcc
     2821 gtccgcgtca tggctgggaa ccggggcttt aaacagaatg gttcccattg cgtaatcgat
     2881 gtcgtagtcc gatccgttcg atttgacttc ccggttcaga acgcggtcag gctgcagccg
     2941 gtcgcgggtt tcgatgacca ccctttccga tccttcgatg atcgtattat tgttgagata
     3001 gtagtatccg gaaatccctt tgccgggcag ggtatcgaca aactggctct ggtcggtgtg
     3061 cgtgccgaaa gagcgcaggc ggaaccggtc ggtgttgact tcggccgaaa gcccgttgaa
     3121 ggatcgcgtg taggcggcaa gcgtcgtctc cgtcaggtcg gtccggtaat cgccgtagag
     3181 caaataggat ttgccttttt ccagcttgac gtagagcttc tcgcgggaaa gagcctcgta
     3241 gcccgttttg ctctcatcgc cgtaaatggg atacttttct tccgaatcca gccgcgtgtc
     3301 cgattcacgg aacagctcat cggtcttgtt cttgttcgag tcataagagg ccgtcagcag
     3361 gaaatcctta aaaacatttc ccttcatgaa gaaagcgccg cgtccgttca catacgcgcc
     3421 gtcattgaac caggaccggc tcttgatgta atcgagatcg ccggatgacc ggccgttgcc
     3481 cagcacaacc tcgccgacgc cgaccatcag catgggccgg aggtgcggaa gaaaatagat
     3541 ctttacggat tcgctgacat cattcacctg aatctcgatt tttgcttcgc ccgtttcccg
     3601 gggagagacg actgtaaatt gagcgacgcc gttccggatc agaatctgat ggccatccgc
     3661 caacggatcg gcgtccttct ccagaatctc gccggcggaa agcgacaccg cggctgatcc
     3721 gctgaaggtt gcaatttgac cttcacgatc ttccagcgat acgttgacgc tgatcttgga
     3781 ttgtccatcc gccggggctt cggtgcgctc gggacggatg accatccgcg ccgcatcgcc
     3841 cgccgccttg atccggattg tctgtgagct ccggacattg ccgaaaggat cacgaacttc
     3901 ggcggtaatc aggttttctt ccccggtctt gagcggaatg ttaataaatt cataaatggc
     3961 cactttgccg ggagcatatt tgatctgttt gccgatttgc cggttgtcca ccttatcgcc
     4021 gttgacggtc agggtgagtt ccgtatccag gggcgctttg acgaccacgc gaatgttttt
     4081 ccgcgccgcg acctcctgat cccgaagatt cagaaactcc aggtccggtg aaaaagtctt
     4141 gattctctct tcgagcccgg cttctttctc tgcagaaact ttttccgcac cggcgtccgc
     4201 ttcgttttct tcctttgctg cgggccggga ctcggccgga ggcgtctggt ctgttttcgg
     4261 ttcggcggat gtctttaccg ccggcgcttc tttggttgca gccgcatcag gcattgccga
     4321 cgacgccttc gcttcttcct gcgggtcgga ggcaaccctt gttgaaacgg atgccttcat
     4381 ttcctctggc tttttgacag gcggtccctc aacggttgcc gcgcccgcag gcgatgcttt
     4441 tgaggagacg gcttttcggc ttgcgggaac tgaagaaaaa atatccgaga gcgcctccac
     4501 aagcgttctg ccctgctgtt ccgtattttc ctgttcctgc ttttccttct gcgcttctgg
     4561 ggcaggcgcc ttcaagatca tggagccttt ttgaaccgca aaattcgcct tgaagagacc
     4621 gccgggcgtc atatcgacaa actgcgaagc gccgtcgcgc ataaaacggt tattaagcgg
     4681 gatcagcttt gcgcctgcag gcagcgtggt ctcgtcaagc cgcagcacat gggttcccgc
     4741 cgagactccg gaaatggaga agcggccgcg cttgtccgtg accactcttg tgccgtcttc
     4801 caggtagagc gccacgtcgg gaattcccga atcctttccg gccagccggt tttcgtcaag
     4861 gtccaggaaa accctgccga tgatggttcc tttcgacgtg aagacgcctt cggtgatttt
     4921 cactttgacg ctggccgtgt tggaaacgac cgtcttgccc aggctcaagc cggccgccgt
     4981 cgccgtatta atccctgttc ccgcctgact gtccggcccc accagcacgc gataggcaat
     5041 ctccaccgac gcggcgggcg ccaggctggc aatggtccag gcaaaggtcc gcttgcccgc
     5101 cgtttgcgga tcggcggatg ccacgccgtt cgttttggtc gattgcgcaa tgtaggccag
     5161 gccgtgaggc atgatgtccg taattgtaag actgtttacc ggcgaggatc cgttgttcgt
     5221 cagattgagg acataagtta gcatatcccc gatcgccgcc gtcgttttgc ttgccgtctt
     5281 ggcgatggtc aagcggcctg tgggggggtc caccggaata tcgcggatga gaggaggatc
     5341 acccacgttc aaggtgaact tttcaccccg tgagccgttg ccgatcgtaa agccggcagg
     5401 cagatcgctc gcggcaaccg ccgaggggaa ggtgtaaccc gcggaaggcg tcacctggaa
     5461 gcagaagtct cccggcggaa ccagcggaaa ggcaaacttg ccgtccgcgc ccgtgggaac
     5521 ggccggattc acttctcccg gcggcagaac cggccagctg gtcaggtcgc aggaattggc
     5581 aacgccgttc cagttgcgca gcgtcaccac ggttcccgcc acgggagcac ccgtgacgga
     5641 attgaaaacg atgcccagcg gatcgatcag ggccgatgcc gtggaaacga ctacggcatc
     5701 gagcgcatcc gagtaggttg ccgttacccg ggagtttgcc gacaccgtca agattccgct
     5761 attggcggcg gatgcggcca gagaactggt cacgccgccc cggaagatgc ccgtatccgg
     5821 ccccgtttcc gtcatgataa cggtttccgt gtcgccggtt tttgaatccg tcaataccac
     5881 cgtgacggtt tcggcgacca gcggattcag attctgatcc gcgtccagaa cctgaagatt
     5941 gacggcattg ccgcttcgat aggcataggc cggctgccag gctgcgtcaa agaattcaat
     6001 cgtgccctgg gttctcgcgc tgaccacgga gactgcacgg accgtccggg ggggcatttc
     6061 attggaggac gccgtggcgg tgttttcgat cacctggccc tggaccaggg ctttgtccac
     6121 ctgcaccgtc agggtgaccg tttgtttcgc gcccgccgcc agcgctccga tggcccattc
     6181 cacctgtctc gcgccggccg cggcaacgcc gccgttgccg gctgatacaa aggttgtccc
     6241 tacgggaatc ggatcgctaa ggacgatgcc ggtcagggga atgctgcccg tgttttccac
     6301 ctgcagggta tagacgatgt tgtttccggc gcggacaggg tcgggcgtgt ccaacttcaa
     6361 aagcagcagg gacggggtcg agacgttcgt gaccaccgtg ttggaggtct ttgtctgcgc
     6421 ggtgctgaga atggaagccg tgttgctgat ttgctgaccg gaaacagcca ccggagaaac
     6481 acgaacccgg aaggaaacct cgcccgcgcc gcccgccgcg acatcgccga tattccagga
     6541 aatcacgctt cccgtcaaac tgccgaacgc gctcgccgat ccggcaacat aagccgttcc
     6601 ggagggaata gagtccacaa tccgaacgcc gtttgccgtc tggttgccga tgttgcggta
     6661 ggaaagagca taggtgatca cgccgttggg cgcaaccgtt tcgggcgcgc cggatttttc
     6721 aaaaaccaga ttgcttcctg cgccgacggt cgtggtcagc gtcgccgaat tggcagcccc
     6781 ttcggcggat gtcgtggaag cggtgaacgg aatcacggtt cccggaagcg ttccggccgc
     6841 gacgacggcc gtaagggtga aggtcacgcc gccgcccggc ccaatggcgc ccagattcca
     6901 cgtcacgagc gggggtgagg atattcctcc gtttgacgcc ccctgaaact ggagaatcgg
     6961 agaaagggtc gcggcaaccg tcacagcgga cgccggaagg ttgcccgtgt tggaaacggt
     7021 gatcatatat gtgagcgtgc cgccgggtgc ggtgggatcc ggagtggcaa cggccgtgat
     7081 cgcaagaacc ggcgctccgc ctatagcggt ttcaacgcga ttggaaggga catccgggaa
     7141 tgcatacccg tttgcccccg taaaacgggc ggttgccgtg ttcgggatga ccgtacccac
     7201 gggcggcgaa agcgccagcg cgaaagcagc gcaaaacgcc agcagcatcg cggctccaag
     7261 aaccgcgatg gcccatcggt taacccatct tttcgacaac aaacctgtca tgacatccgc
     7321 cgattgccca cgttccgaat ctttgctccg gcggggattt gctgcgctgc tttacaacga
     7381 aacaaatcct ccgccttaaa agcgattatt taatggtcgc cctgtacacg atcggaatcg
     7441 tggcgcctgc ggcaatgctc gggatcgaaa tcataaccgt gccgctgact gcgtcataat
     7501 gcccgaaatc gccgtcggcc gcgtcggttt gccccgcgcc ggctacggtg atgctgttcg
     7561 gcacgtaatt caggtagctc gacagcacgt cggtgacaac gaaggtttgc gcgggcgccg
     7621 caccggtgtt ggtcacggtg atcgtatagg tcaactgcgt tcccggaggc tgcgggccgg
     7681 cgggtgaaac gctcttgacc agccccatga cgggcgtctg aatgttggtg aacttgatgg
     7741 atgtccctgt gactgttgta tcgtaaacgg aggtcgcggt gatcgtatgc ttgccctgct
     7801 gaccgttgga accggccacg gtgacgacgg cgacgtagaa cttggtttcg tcgggggcca
     7861 cggcgcccgt gtcgattttg ccgtcggcgt tgctgtcggt gacgggcgtg tccacgccgg
     7921 ccgtgtattt gccgtcctga ttcacgtcat tatacacagc ccaggtccag tagagaccga
     7981 cggagctgat gctgtagata tccgtgctgt tgccggtatt cttaatggtg aagggatgca
     8041 ggttctggtt tcccaccagt tcgtcggttg tcagcgtcgg gggcatgacc tgcacaccgg
     8101 cggcctggga caccgtaaaa ggagacgtat tggtattgac tgtggtctcc gtcccaggat
     8161 cgcctgcatt ggtccggtag gtggccgagg ccgtgttgtt gatcacggtt gacgcagcga
     8221 tattggcgtt gactgtgacc tggaagctga aggtcccgga gcctccggct gctacggtgc
     8281 cgatcgcggc agtcactttg cccggattgg tgacgttgta gtccgcgttg tccgcgtcgg
     8341 ccgcatcggt gcgggccgca ccgctgtaag tcatggagcc ggttttgtag gttgtttcag
     8401 ccggaatggc atccgtcagc acgaccaggt aacccggcgc cgtgccgctg ttgttccagg
     8461 taatggagta cgtaaccgtt tcaccgggtt tgggggcggc gggcgttgcc gatttactga
     8521 aggtgatcac ggccgcctgg atggtaacgg tcatcgtgga ggttgagctt acggccggcg
     8581 tcaatacgga tgttgcggtg aaattgagcg aggcggtgct tcccgccgcc gctccggccg
     8641 gcgccgtaat aaccgcgatg gcatattgaa cctgatccgc gtccagcgag acgcttgcgc
     8701 ccagcatatt gccggcgacc tcttccgacg gctgcagaat gccgtcgccg ttggtgtcct
     8761 tgtaaatggc ataggtatag ccggccggaa gtccggcggc ggtcagattg aaggtgtccg
     8821 tcccgttgcc ggagttgacg atctgtccgg aaaagtaggt tttgccgccc ggagagccgt
     8881 tctggctgac cgtggcgggt gacgtggtca cgccggccac ctgactgacc gttacgctca
     8941 cggtgttgga ttcaaccggc gcgtaggtgt tgttgttggc gtcggtgtaa gttgccgtgg
     9001 ccttgttggt aatcgtagtg ccggcgggcg tgcccgcggc aaatgccgtg ccggcggaca
     9061 gcatcgtgga cagcagcagt cctgcgatca gcaaaacgac gaacttaagt ggtgctttta
     9121 ctgttttcat gattgttctc ctcccttggg ttttatgttt ttcatcagtt tcttgtgctt
     9181 atttgacttt aactttaaag gtcaccgtcc cggtcgcgtt ggccggaacc ggcgccttga
     9241 tgatccattt aatgtgagtg aaaagttccg gcggagcggg ttgaaggatt tcctttccgg
     9301 ctgcgtccag gcttttgcgc aaaacggggg gctccacaaa agtcgccccg ccgttgatgc
     9361 tgaaaaagat ctttgcgtcc tttccggctg cgcttcccgg cagatacacc gtgtttgccg
     9421 gaacgggatc gacgaccgtg acgtctcttg ccacactctt gccggtgttg tgatacataa
     9481 cggtgtaaac gagaatatct ccgcttttaa ttttgtccag cggcgccgtg gttacggttt
     9541 ttttgccgtt ccggatttgc gtcacttcct tttcgccact gactttgaga gcgatgcgcg
     9601 gctcgtcggc ccgtgcttgc ggtgccgcta gtaaaagcgc cgccaagaac agcgctcccc
     9661 acaggaccat ccatcgaaat gtttttacgt tagtcataat gcctccttat tgcttgcggg
     9721 tattttaaaa acttcctgtt atcgtagaga aaagctgttc atcaatcttg tccctccttc
     9781 gcgaatccct ggcgttatcg gatcctcacc ttgaaataga cttttccttc atcgttgcct
     9841 gcgttgtccg caccgtcatc acccgcgact gtcgagatga taaacgtgac attttccccg
     9901 ttgatctgac cgacatccgc atcggcgata tcggtcaaac tgtttgccgt ggcgtaggtg
     9961 gaagcggcgt tcccgctttt aaggctgccg gttacataag ttgtgttcgg gggcaccgta
    10021 tcgaaaatga tcagattcga ggcggcgctg cctccgacat tccggtaata aatggtaaag
    10081 aggacatcct gacccggagt agccgttgac ggatccacgt ttttggtcaa ggtaatgtgt
    10141 ggcatggttg tatccgtatc gtccgcagaa ttgttgcccg gcacgggatc ggtcacaccg
    10201 gccggcgggg aaacggttgc cgtattggta aaagagcccg caaccgccgg atccaccaga
    10261 accgtgagcg acagagcaat gctctgtccg ggcatcagat taagccccgt ccagagtccg
    10321 gttgcttcgt tgtaaattcc ctgtgacggt ttgaataccg ggctctgcaa aagcggcggc
    10381 agggtgtcga tgacggtgag actctccacg cggcttggac cgttattggt cacggtgatg
    10441 gtgtaagtaa tgacgctgcc gggcgtgacg gatgtttttc cgtccgtttt tgtaatggcc
    10501 agatccgcct gataggtcag cgtatccgtg tctgaggcgg tattattggc cgtattggtg
    10561 tcctggacgc cgggcgacgg cgacacggtg atcgggaagg tcagcgtccc ggtggccgcg
    10621 ggcgaaatcg tcccggcaat ggtcatggtg acgctcgccc cggcagccag gctcaggcct
    10681 gtccattcac cggtggcggc attgtaactg ccagacgagg ggcttcgggt cgggttcaga
    10741 atcgtcgcgg gaatcgtcgc gtccagattc agactgatga caccgtccgg tccgttgttc
    10801 gttacagtga cgatatagct gaccggcgat cccgccacgg cggttgtctg gttgtcggtg
    10861 atggcaacgg cgaggtcggc ctgcacgggc ggattggtca gcggcacggt gatggacggg
    10921 gcgggtcccg cgccatccgg atcgatggtg gcaacattcg tgataatgag cggcggattg
    10981 gcattgatgg tcaccctgaa actgaccgtt gccgtttctc cgatcttgat ttgcccggaa
    11041 gcgccgccgg gactgttcac ctgacgggcg gtcgcgaagg gcatggcgcc gccgacatcc
    11101 gccacgacgc ccccgttgag agttgtggag ccgggaacgt aagtggttcc cgcggggatg
    11161 ggatcggcaa acgtcgtacc gctggaatta gcggttcccg tgttgggaat ggcaatcgta
    11221 taagtcaccg ttccgccggg caggaccttc cctgaagggt tagccgactt tgttggctgg
    11281 agccggaccg taccagtcgt gctgatattt gtggtatttg tcccgttgtt gagaggaaat
    11341 cccgcacagc tctggtattg ataggcccag gaggattgcc cggcgtaggt cgccgctgcc
    11401 gggggcagag ggatagacgt cacctgcacc tgatatgaga cagtcacggt tgccccggcg
    11461 gccaccgtgc cgatattgac gccgcttacg ggatttgctc cggactgagc aacgccgtta
    11521 actttaaagc tgccggatat gaaggctgtc ccgccagcca gggaatgtgt aaagacgaca
    11581 tctagggcat cggctgatcc cgcggaattg ttcagaatgg ccgtgtaggt cagggtatcg
    11641 ccgatatagg tcgtggcttt gtccaccgat aagaccgctg aaggaaaaac cggcgctccc
    11701 acgttgatct gcaaaccgat cgtggtgatt gtataacggt cgctggaggt cgttccccgg
    11761 gcgaaggccg tgttctggtt gctctgcatc cgggcggaaa catctacatt ggtaatgtcc
    11821 cagcccgctc gcacgccgtt tccctggctg ccgggtgtgc tgttccggtc accgaaagtg
    11881 cctgccgtat cgcgcgcgcc gctgtcctga ttaatctggg aggcgaagaa attgctgctc
    11941 gggttattgg ggccggagac ggcggccagt gtgcccgccg tcgggccgaa acgcatctgg
    12001 tcgccggtga tgcctgaatc cccttcgatt gccgaaacca tcatccgggc gttgattgtg
    12061 cccgctccgg gggtacagaa accgctgacg gaggaagtgg tggagaccga ggaactggtc
    12121 agctccgctc cgacaaagat agtcatattg cgggctggaa gcgacgcgtt gccataaatg
    12181 acggccagcg tccagcccgc ggcattcgca ttattctctg ttgcggcaac cgtggctggc
    12241 acaccgccca ccgtgtaggt tccggcgcct ccggcttgaa ccagagacgt aacatcggcc
    12301 gaccggacat agtaatattc gcccgatccg gtgacggccg ccgtggtcgg agaagggctg
    12361 acgctcatgc tgcccgctgg cccggaaaag gttacggcgc tattgagaga accgctgacg
    12421 tcctgccctc cgtaacggta actgccgccc cagacaagtt cggcgtagat cacgctgctt
    12481 ccggcgggca ggcggagtat ggcggaagat gaatttttgg tccagttcag tgtggttccc
    12541 gcggggaaat tgcccacctt gaggctggtg tccgtggtaa tgaatgcgcc gatagcgcca
    12601 ttggtgcccg gctggttgga gttggtggcc ttgcttaagc ccagagtgtt tcccgtgtag
    12661 gtcattccgc cgttggcgat ggttgtgtaa cgcttcacat aagcggcgtg gaggggttcc
    12721 gagccaacgg ccatgaagag acagacggtc agcaggtaaa acgccattct gcgtgccatg
    12781 gcacgcaatt gccgggggcg gggattttcc atgaaattaa aaaatttttt catcttccat
    12841 cgtcattggt tttttatgtt gctatccgca tgtttcataa aagcaaagca aatgaccaaa
    12901 gctacttgtc tttcaggcag agagatcctt gtccttcttt agctttatta tggcactgcg
    12961 ggcaaagtcc ataaacttta acggatattt cttgaatctc aaatccctga aggtgctcgg
    13021 gaaaaatatt gatggcgctc gtttgatcaa tgtcgacaat caacccgcag gaggtgcaaa
    13081 tgaggtgatc gtggggagag ctattgggat caaatctttt tccggagctg ccgtgtaccg
    13141 gaatttcttt caaaagtcct tctttctgca gaagcatcag cgtattgtaa acggttgtca
    13201 gcgagatggt gccgatctgt tcggataccg ccgcataaat gtcggcgacg gacgggtgat
    13261 tacgattgtc cttcaaatag gacatgattg cttctctctg cgctgttttc tgtgctttct
    13321 tcaacatatc ttaactccta taatattttg accgttataa atttgaaacc ttcacaattt
    13381 tggaacaatt acaaatttga attaatggaa gactagcggg aagtcaagca ttatttttct
    13441 aaataaataa aatattcagc gcctatcgat ggttgccggc gatcgttcat gagccatttg
    13501 cgaatcgggc gattaacgta aatttataag ttatatcaat tatttatatc atactttcct
    13561 cctgcgcggc atttcgggaa tctaaaagtt gataaaaaac cgcaactttc ttcattcgga
    13621 atttcctgtg atgcattttt gattaaaatg tgttagcccg aacatcgatt attggttgcg
    13681 ctcataccgg caaaggggga agacccttga tcgcagccag gtcaacacat ccttataata
    13741 gaaaggaatc agattatgtt tgattatctt ttatccaaag aacagctcaa aatccgcgat
    13801 gaggcgcggg aattcgtcaa gtcgattccc cgtcagacga ttctggacat ggacgcggat
    13861 aaaatcaagt ttcccaagga attcttaagc gaagccggaa aaaggaatct gctgggatgc
    13921 cgctacccaa aaaaatgggg cggacgggat ctggactggg tcacgacctg catgctcatg
    13981 gaggaaatcg ggactctcgg ctacgaaatg gcctgcgtct tcggcgtggg ggcggagctg
    14041 gtctgcgatg cgattatcct tcacggcacg gatgaacaga aggagaaata tgtcaaaccg
    14101 ctcctgaggg gcgatctttt tgccgcggag tgcctgacgg aaccccgggg cggctccgac
    14161 tttttcggag caaccaccaa agccgaagac atgggagatt acttcctcat caacgggcag
    14221 aagcgcttta tcgtcggcgg cgagggagcg gatttctttc tgctttacgc caagacggac
    14281 ttcgacccca aaaccaagcc gcaaaaatcg attaccgcgt ttatcgtgga ccgcggtccg
    14341 ggcgtggaaa cgaaatacct ttacggcctg atgggctgcc ggggcggagg aacggcgcgt
    14401 ctggtcttca aggacgccaa agtgccgaaa gccaatgtcg tcggcaaggt caacggcgcc
    14461 tatgacgttt tcaacaccat gatgattccg gagcggctcg gcacggcggc catgaccatc
    14521 ggcgcggcgc gccccgccct ggaagtggcc acaaactaca ccacccggag aaaagccttc
    14581 ggccaggtca tcgcccagtt cgagggcgtc agttttcagg tggccgaggc gatcatgctt
    14641 ttggatgccg cccgttcgat ggcctacgta acggcgcgcg cggtggacgg cggcgtcaac
    14701 atgaaccgca tccggcgcat ggtgtcggaa tccaagaaat tcatcaccga gtcctgccag
    14761 aaggtcgttc acaattccat gcaggtctgc ggcggaatcg gctacacgaa cattctgccc
    14821 atcgaacgca tccaccgtga cgtgcggctg gcctccatct ggaccggaac gagcgaggtc
    14881 atgtccatga tcaccgccca tgaatggtac agggaatatc tgatggcaaa agcggcgggc
    14941 ctgacccgtg actgtgaact cgatgccgcg gaagccgaag ccgcggacga aatcatttac
    15001 gaataacaaa aaaaaggggg tatcacggcg agacgcgatg cgtgcgccat gatcccctct
    15061 tctcaccggc ctgcggcggc gtcagactct tcaatgattg gttgacaact tttctgaaaa
    15121 cccattataa aagttgccaa caagttgtat gacggaataa aatgtaattc gcttattccc
    15181 actcgatcgt gccggggggc ttggagctga tgtcgtacac gacgcggttg atgccgcgca
    15241 cctcgttgat gatgcgggtg gaaatacgcg cgagcaggtc atgcggcagg cgggcccagt
    15301 cggcggtcat ggcgtcgtcg ctggtgacgg cccggatcgc cacgatgttt tcatacgttc
    15361 gctggtcgcc cataatgccc acgcttttca gcggaagtaa aatcgtaaac gactgccaca
    15421 atttgccgta gagattggcg tcgcggattt cctgatgcag tatccggtcc gcccgcttca
    15481 gaagggacag gcgcggaacc gtcacttccc cgatgatgcg gatggccagg cccggtccgg
    15541 gaaaaggctg ccgccaaacc acgtcgtccg ccagccccaa ttcctttccc aggagccgca
    15601 cttcatcctt gaacagatgc ctcagcggct ccaccagctt caatttcatg gttttgggca
    15661 aaccgccgac attgtggtgc gacttgatca ccgcgctggg gccgccgaaa gcggaccgcg
    15721 attcaatcag atcggggtac agcgttccct gcgccagaaa ccggacatct ttcagtttgc
    15781 gcgcttccgc gtcgaacact tcaataaacg tccggccgat gatcttgcgc ttcctttcgg
    15841 gatccgtcac ccctttgagc ctttgaagga atattcttcc cgcccggaca aaccgcaggt
    15901 tgatgcgcag gtgcttcttg aacatgtcga tgacgcgctt tgcttctcct tcgcgcagaa
    15961 cgccgttgtc cacaaaaacg caggtgagct gtttgccgat cgcccggtgc aggagcgccg
    16021 ccgcgaccga cgaatcgacg ccgccggaaa gcccgagaat gaccttttcc tttcccacct
    16081 gccggcgaat gtcctccacg gcctcggcga caaaagaccg catcgaccag gtcctggcgc
    16141 agttgcagac cccgaaaaga aaattggcca gcattttccc gccctcgcgg gtgtgaacca
    16201 cctcgggatg gaattgcagg ccgtaaaagc gccttcgggc gtcctcggcg gccgcgacct
    16261 tcgtgttggc cgtggaagcc gtgacggaaa agccccgcgg cagttctccg atggagtcgc
    16321 cgtggctcat ccagcaggtc gttttcggct tcacgccgga aaagaggcct ttttgttttt
    16381 tgatggtgag ttcggcgaaa ccgtattccc gtttggccga gccgatgacc cgcccgccca
    16441 gggcgtccac catgaactgc aggccgtagc agattcccag caccggaatc cccagctcaa
    16501 agatgccgct ctgtgcgcgc ggcgcgccgg cggcgtaaat gctggccggg ccgccggaca
    16561 gaatgatgcc ttccggcgcc atcgcccgaa tggcggaaag gggaagttcg ggcggctcga
    16621 tccggcaata cacctggttt tcgcgaaccc gccgcgcgat gagctggttg tactgcgagc
    16681 cgaaatcaat aatcagaatc atgagggggt tcctgtcttc tcagcagata aagagcattt
    16741 cccagaattt ggggagcggc cagatttcat catccacgat attttcaaga gcgtccacat
    16801 attcgcgaag ctcgtccatt ttcggtttga ccctggcgca aagcgcctcc gcttttttct
    16861 gctcgccgtc catcgccttg gcggcctgca ttccggccgt gatttccctg cacgccgcgt
    16921 atatgtcgtt gatcaaatcc agaattttct tcaaaagctc cgcctgggag gaaatcaccg
    16981 cggcggaccc gaggacttct ttggcgtcaa gaatcgcccg cgccagtttt tgctgatagg
    17041 ccgcggccgc cgggatcacc tgcgtcagac agatgttgat gaggcagttg acctcgattt
    17101 ccagatcctt gatgtagcgc tcggtgtgga tcaggtagcg ggctctcagc tcctcgttgg
    17161 aaagcacccg atatttttca aaaagacgaa gcgcccggtc ggtcaccagg gctttgagcg
    17221 cgccgggtgt tgttttttca ttgggcaggc cgagcttctt cgcttgggcc gcccattcct
    17281 ttgaatagtt gtccccgttg aacagaatcg gccgaagctg cgcgatctcg tttttcagga
    17341 cgtcaaaaac cgcttggttg atgttcttgg atcctttggc cttgatcttt tcggccagtt
    17401 cgtccaggct ttccgccacg atcgtattga tggcgtaggc ggggcaggca acggactgag
    17461 acgagcccac cgcgcggaat tcgaacttgt tgccggtaaa ggcaaaagga gaggtcctgt
    17521 tgcggtccgt gtaatccttg ctgacctgag acagagagga aatgcccaga tcgatgattt
    17581 ccgcgttggt cgctttgtgc acgatgcctt tttcgatatt gtccaggatc tgcgtcaact
    17641 gttcgcccag gaacacggag aggatggccg gcggcgcttc gttggcgccc agacggtggt
    17701 cattgccgca ggatgcgacc gtggcgcgca gaaaatctcc gtgccggtaa acggcgcgga
    17761 ccgcggcgat gagaaacacc agaaaagaga tattgtcctg cggtgttttg ccggggttga
    17821 gcagattgtt gccgcggtca tcggaaagcg accagtttac atgcttgccg gagccgttga
    17881 ttttggcaaa cggcttttca tgcagaagcg cggaaaggcg atgctttttc gcgacgtgct
    17941 tcagcgtctc catgatcatc tggttgtggt ccgtggccag attggcttcc tcaaacacgg
    18001 gcgcgatttc atactggctg ggcgcaacct cgttgtgcct ggtcttggcc ggaatgccaa
    18061 gcttgtagag ctcttcctcc acgtcgtgca tgaaggacga aatccgttcc ttgatgctgc
    18121 cgaaatactg gtcttccagc tcctgtccct tgggcggcgg cgcgccgacc accgtccggc
    18181 cggccagcac caggtcctgg cgcttgtagt agtagtccat gtcgatcaga aaatactcct
    18241 gctcgatgcc cagattgacc tggacttttt tcacgctttt tgcccccagc agtttcagca
    18301 tgcgaagcgc gctttgattc agcgcgcgca gcgaccgcag aagcggcgtt ttcttgtcca
    18361 gcacctggcc cgtataggaa ataaaaaccg tcggaatgca aagcgtcgtg gagattccgt
    18421 tgcgcttgat gaaggcgggc gaagacatgt cccacgccgt gtagccgcgg gcctcaaagg
    18481 tcgcccgaat gccgccggag gggaaactcg atgcgtccgg ctccccctgc accagctgct
    18541 tgccggaaaa ccgctcgatc acagcgccgg ggccgcaggg atcggcaaag gcgtcgtgct
    18601 tttcggcggt gatgcccgtc atgggctgaa accagtgcgc aaagtgggtc gcgccctttt
    18661 ccagcgccca ctccttcatg gcgtgcgcga cgacattggc ggtctcttcg tccagcgact
    18721 tgttttcagt catcgcctgc atcaattttt tgaaaacatc tttgggaagc ttctctttaa
    18781 tgaccgcatg gctgaaggtg tcctcgccaa aatactgcga tacgggaaca aacttttcct
    18841 tttcgaccgc gtattgcctt tccgccgcga gcgtgggttg catctttttc atggctttgc
    18901 cgtccttttg cgagagatgc tcctctttac tccaatagcg gaatttgcgc aagcacccag
    18961 cttctttccg gctttttcct tccccccgaa aatttcaagg gctgcaggca tcccccagaa
    19021 aacagatcat cttctgggtg acgatgtccg acatcaggcg cacgtccttc gcccggacgt
    19081 cacagacgcg gacaacgccg gcaaaccgcg catcgtccgg catcaggtcc gccagaatat
    19141 tgggctgttt tttgatccac tgcacgttga agaccacgcc ttccttttcg ggataaatgg
    19201 ccatgtaaaa gatgtccatt tccaccatgt cctggaaaaa gtgcgttccg aaggacaaat
    19261 cggggatcag ggcgccgtcc tggtaggcga tctcgccgat ggcggtcatg ttgttgattt
    19321 ccgaaaacgt aaccggaaca cccatcgccg gcgtggtcgt tccccatcgc cccggcccca
    19381 gcagaaccgt cggcatggcc tcgcgctttc caatccgccg gttgagcttg ccggtgagcc
    19441 tggcaatctc atgcttttcc gtaagcggca gcccggaata ggccctggga tcaatgtaga
    19501 tcacgcggga gatcgcctgg taaacgctgc cgcccataaa gttggcgtcc tgccggatca
    19561 gcattctgga gcggctgatt ttttccggca gctccacccg gctgtaatgg cctttggttt
    19621 ggaaaggacg gcactgcagc aggttgatcc gggtttttcc ctgcgcgtcg aaattgacgg
    19681 tgaattcgat gtccaccgga tagcggtaaa cctgttccag cgtcttgagc attttggaca
    19741 tggttttcac aaagggcgtc gcagacagca gttcctcaaa cgtgatgatc caggcctgct
    19801 tcgcgtcccg tcccacggag cgcagataat cggccgcttc cttatcttcc tccacgatca
    19861 aattcagatg cgccaccaga tcctcacgga gcagttcggc ggtctggagg gtttgaaatt
    19921 cattgtcgcg cagattcagg acatccgctt cgtgctggga aaaccgcttg atatcttcgg
    19981 gtttcgtgta gggtttgacg gtgggcgcgt ccagggcgac gatccgtgga taatcatttt
    20041 ccacgcgatt gacggcgcgc gtgccgagac cgaacacgat ccgcagcatg ccggctttcg
    20101 ggtccatccc tttctgccag acgaaggtat tgtaggacag gccgacgccc gccagaccgg
    20161 gaaagaaata acggtcgctg tgcgaaccgg aaacgcgttg caccagaagc gccatctgtt
    20221 catcctgctg ggccatgccg cgctggaggc ggtaggtgag cgcgtcctcg ttcatcgtgg
    20281 aggcgaagat tttgcggacg gcttcctcga aatgccggta gcgttcctcc ggcgttccct
    20341 ggttgacgca gaaatcgctt tcgtattttc cggcaaaggc gttgccgaag gcgtcttcca
    20401 gaagcgaact ggagcggacg atgatcggcg cctggccgaa atattccagc atgagctgga
    20461 actgctcctt gatttcgtcg ggaaacacgc cgtgcagcat cttgtttttg agctcgcgcg
    20521 cgacatcaaa atatccttcg tcggttttct gcgccatcag cagcttccac cagccgttct
    20581 gcaccatgta ggaatagaaa atgtccgagc cgacatagaa ggaatcgtgg atttccagaa
    20641 cggcgttcca gtcaaaggac ggatccttgc gcagaatgtt gcgcgccagc agcatgccga
    20701 cggatttccc gccgataaac cccgtgccga taaggcgctt cttgatttcc accaggtctt
    20761 ccaggtcgaa gtattcccgc accagcgaca ggatccgctg ctccctgccg atcatcacgc
    20821 gggaaagaat ttccaccatg tcctgctttt ccggggccgc gtcgggattt tccagaaggc
    20881 tgccggcttt cagaaagatg cggtcccagt aatcgagatt gcgctcgggg ccggtggcat
    20941 tcctgtccgt caggcaggac agaaagctcg cggcgtccgc gctgtttaaa atcggaacgc
    21001 aggcctggtc ttccatgatg tgcggcagaa acatggtggg cgtgtagcgc ttccaggcct
    21061 tgagcggatg gacgcagagt cttccttcgc ggttgcagat atccagcaga acctgcgtcg
    21121 tttcgcggat ccgggcgacg gttttgaaag aatggcggct gcgcaggatg gcaaaatacg
    21181 cgaccgtgtt gagctcaaaa aggtaagggc aggtgatgac gaagaagttg ccgatcatca
    21241 ggtcggtggc ccaggcctgc agcagatcgg acagacagtc aaaaacgtaa aacacgtcgc
    21301 ggccttcgtc gcggatgatg ttgtggacct ctgtggaaaa ggattcaaat cctgaatttg
    21361 cgttgagttg acagattttg attccggggc gcgcctccag aagggcgggg tggttggcaa
    21421 aacgcatgta aacgaggcgc tgacgatcgg ccagggcctg ctcgacatag ggatcgacaa
    21481 attttttata gtcctcgata tggtccacct gccagacgac attgtcgccc atctgcagat
    21541 agttgaggat tttatccaga ccgctgatgc cggaactgac gcgcgcataa gggatcataa
    21601 cattctgctt ccttttcttt gcaaatctta aacgtctctt tcaaacggtc ttcaattttt
    21661 atgccaccgg cgttcgggcg catatccttc gtctttttta cgggtcattt gccgaacatt
    21721 tttgaacaaa tttccagcca ttccggcaag acgccacgta cacattttga ttctgattct
    21781 tgataaatca cacaatattg ttcttatttg aaacggccat tgatcgataa attatctgtg
    21841 atttcaatat tcaagaagtt gacaatcctg atggcaccat ccttgctcaa gtcaaaaaat
    21901 gaaaaaattc ttacaggagg tccaccatca tgaattccgg agatacagcg tgggtgttaa
    21961 ccgccggcgc cctggttctt ttaatgacca ttccagggct ggcatttttt tacggaggtc
    22021 ttgtccggcg caaaaatgtt ctttccattt taatgcagtg cttcatcatc gtctgcgtca
    22081 tttccctgca gtgggtgctc ttcggctatt cgctggcctt cggtccggat tttcacggca
    22141 tcatcggaac gctggagtgg gcgggactgg ccggtgtcgg cgccgctccc aacagcaatt
    22201 acgcggcgac cattccgcac agcgtcttta tgatctttca ggcgatgttc gccatcatca
    22261 cgccggcgct catcatcggc gcgtatgccg agcgcgtgaa attcccggcc tttctgctct
    22321 ttacgctctt gtgggcgacc ttcgtttatg atccgctggc gcattgggtc tggggcgcgg
    22381 gcggctggct caagtctttg ggcgggctgg atttcgccgg cggcatcgtc gtgcatgtga
    22441 gctcgggaat ttcggccctg gtgctggcgc ttctgctggg aaagagagtc ggctacaacc
    22501 acaaacccat tcgtccgcat aacctgccgt tcaccgtttt aggcgcggcg cttttgtggt
    22561 tcggctggtt cgggtttaac gcgggaagcg ctctggcggc cgacggcctg gcggccaacg
    22621 cctttgtgac tacgaatacg gcgacagccg ccgccggttt gacatgggcc ctgattgaat
    22681 ggtggcacaa tggaactccg acgattctgg gcgtggccac cggcgccgtc gccggtctgg
    22741 ttgcggtcac cccggcctgc gggttcgtca acccgatgaa tgccatcttc atcggcatca
    22801 tcgtcgccct ggtctgctac accgccgttg ccgtcattaa aggcaaactg ggttacgatg
    22861 attctctgga cgccttcggc gtgcacggcg tgggcgggac ggtcggaacg atcgccacgg
    22921 gattattcgc ggaaaaagcg gtcaatgcgg cgggcgcgga cggcctgctg ttcgggaacc
    22981 tgcaccagtt cggggttcaa tgcctgatgc tcgtggtgac catcgccttt gccgcgatca
    23041 tgaccttcat catcttcaaa attgtggacg cggcaatcgg catgcgggtc gaagagaaca
    23101 acgaaatcat cggcctggat ctgacccagc aaagcgaagc ggcttacacc gtgattgaat
    23161 aggaggaact ggccatgaaa ttagtcattg ccatcataca gccgcacaaa ctggaggctg
    23221 tcaaaagaga gctggaagcc gtggacgtaa atttaatgac ggtcacgaat gttgttggcc
    23281 agggaaggca gaaaggcgtc acggaagtct atcgcggcgc gaaggaagcg ggcggcctgc
    23341 tcaataaagt ccgcctcgat attgcggtga acgaagcgtt tgtcgagccg accatcgacg
    23401 ccgtcacccg cggcgcgcac accggctcgg tcggcgacgg caagattttc gttgtggagc
    23461 tggccgaatg cgtccggatc agctccggcg agcggggcgg cgttgccatc gggtgagggc
    23521 attttaagga aacaatataa atcatgagga gaacagtgac ccatgaaaaa aaacatcaaa
    23581 agaatcatga tgatcgctct gctggcgttg atgctgacgt ccatgccgat ggcggcgggt
    23641 gcgggggaag aaccccaatc cgccggacca aaacagcagg aagataaatt gaccggcgag
    23701 attgccgcca gtgttttgag cgcctacatc tggcgcggcc aggaaatgac gcgcgacagc
    23761 gtggcaatcg agccgtcgtt tacagcaaac tacaaaggtt ttaccgccgt tctctgggga
    23821 aatctggata cccgccccta tgcggccggg gacggcaaac attcatccac ctggtctgaa
    23881 acggatttca tcctttccta tgcgcatgaa ttcggcatcg tgaaggcggg cgcgggatac
    23941 atttattacg gtctggccgg aatgacgccg ctgggagtgg acctgctgga tgcccaggag
    24001 ctttttgttt cgctgggtct caacacgatt ttggcgccta cgctgaccgt ctacaaggaa
    24061 atcgatcatt accatcaatg gtacgcgacg ctgggcgttt ctcatacctt tgccctgcat
    24121 gaaagggtcg gcttgaaact ggcggcgcag gccagctacc tgaaaagtga agatgagaca
    24181 acctatccga aatttgacag caactcagcg gccaccacgg acaatttcaa caatttccat
    24241 gacggagtcg caaccatttc cctgccggtt gccgtgaccg ggaatctgac cattacgccg
    24301 accgcctcct gtgtgtttcc gctttccgaa gatgcgaaat atgagatgag ggcgcggggc
    24361 ctgaagggca cggccaatcc atccgaccgg gacagttact acgtttatgg cggcgtaaca
    24421 ttaagtttga cattctaggg agagccatga tgaaactggt catcgccata gcacagtccg
    24481 acaagctcaa agacgtcata caggccttac gcaacgtcaa tgcaggtaaa atcaaggaga
    24541 ccatctgatg attgcccgtt atttcaaaca cgcgcccttt gaagatatcg gcagcatccg
    24601 ttcgtggctt acgacggccg gctacaccat cacctgcacg ccgttttttc aatcggcaaa
    24661 cctgcccgct ttggaggaaa ttgacctgct gattgtgatg ggcgggccga tgagcgtcaa
    24721 tgacgaaaag agatatccgt ggcttgttcc ggagaaacag tttatccgtc gaatgattat
    24781 ttccggcaag ccggttttgg gcatctgcct gggcgcgcag cttatcgccg gcgcgatggg
    24841 ggcaaaggtg tatcctaatg gtgtaaaaga aatcggctgg tttcccgtcc atggcgtttc
    24901 gtccggcggc agatccgtct ttcgctttcc gcaatcggtg caggctttcc attggcacgg
    24961 agaaacattt gatctgccgg cgggagcaac gcgcatcgcg aaaagcgatg gatgcgaaaa
    25021 tcaggcgttt cagctgggcc aatcggttat cggcctgcag tttcatctgg aaaccacgcc
    25081 gcagtccgcg caagacctgg ttgcgcattg ccgggatgaa cttatcggcg cgccctatat
    25141 acagaccgag acggaaattt tgtccgccaa gccggcacaa tatcaatcca ttaatcgtct
    25201 gatggacaag atcctgaatt tcctggcgcg aaccgcttga ttatttactt ctgcgttcat
    25261 caatgtcccc gggatacatc atggcgcccg gaccacgctc gcgtgtccga acccgaatga
    25321 catcttcgac aggtgtcaca aaaataatgc cgtcgcccgt ctcgcccgtg taagccgagc
    25381 agaggatcgc ctccagcgcg gcgtccagat tgcgcgtgtt taaaacgatg ttgatttgta
    25441 tttttggaag catattgacc tgataggttc cggcgcgtcc gaccagttga acgccgccct
    25501 gccggccatg tccccgcact tcaaacgtgt tcatgcccac aattccaatt tctttgaggg
    25561 cgtcgataac atcgtttact ttatcttcgc gaatgatcgc ttcgattttt ttcaactgat
    25621 tcattcagtc tgcgtctttc ctgaaaacgc atagcggata caattatgtc tgtctgtgtt
    25681 caaaacggac aaacgtgtat tcttttgaaa atcaacagag ttgctcaacc tattgaatgc
    25741 ataaaacaat tttaaattct tctgctggca cggccattgc tctcggctca ttggtgttat
    25801 ccgtcgggaa aggaagcgct tatcagctta cgctggtttc gttttttcac tgaggggtcg
    25861 tctaacggca ggacgccagg ttctggccct gaaaattgag gttcgagtcc ttgcccctca
    25921 gccatcggct ccattttgaa aggccaatgc tgatgaaact gatcatcgcc atcgtgcaat
    25981 ccgacaagct caaagacgtc aaacaggcgt tgcgcgacgt aaacgtgggc aaaattaccg
    26041 tttccaccgt agtcggctgc ggcgcgcagg tcgggaaaac cgacacgcgc gacggagtca
    26101 ttaccaacgg agacctcctc gaccgggtgc gctttgaaat tgccgtgaac gacgactacg
    26161 tcaagcccac gctggaagcc gtcattcggg gagcgcgcac cggcagcatc ggcgacggta
    26221 aaattttcat cgttcccctg gaggaatgtg ttcgaatccg cacgggagaa accggccggc
    26281 tggccatcgg ataaatcttt acgaaatccc cgcaaaataa cattttcttt ttaattgcat
    26341 caaacggaca taatggtttt ctctttgcat taccgtacgc ggacgaatga aattccggtt
    26401 gatattcgta caattttgtt acattctacg ggccgtgaaa atccggccgt gaagcgccgg
    26461 taaaagcccg ggggagaaag acgacgtgag catccacagt tatgaagaat tagcggccct
    26521 gcacgccata tcaaaaatcc tggcgcagcc gatggatttg agagatcaac tggaaaaaat
    26581 cctccatgaa atgagtttgc gcctcggaat gcagcgcggc atgatctccc ttctggatca
    26641 cgaggaaaag gaagcgtggc tcgacatcgc gcatgacgtc aatattgacg gcatggaagt
    26701 gacgtacaaa ccgggagaag gaatcaccgg ccaggtagcg gagaccggcc gcccgatggc
    26761 cgtggcgaat ctggggcagg aaacccactt tctggaccgc acgggagcaa gacgtcattt
    26821 gaaccgcgcc gagctttcct ttttatgcgt tcccattatc tatgacgagc gcgtggtcgg
    26881 ggttttatcc gccgacaagg tggccaagca ggtcgaggac ctggaaaagg aactggccat
    26941 gatgggctcg gtggccgaac tgatggccaa ggccgttcac atccgtgccc tggaagagga
    27001 aaaccgccgc ttgcgcaaaa ttgtcggaca gcagcacgcg ccttccacgg atattatcgg
    27061 ccattccaag gccatccagg aagtcttcgg cctggtggcg caggtttccg attccaacac
    27121 gacagtgctg atcaccggcg aaacaggcac gggcaaagag ttgattgccc gggcgattca
    27181 caacaactct ccgcgcaaaa gcgggccgct ggtgcaggtc aactgcgcgg cgattcccga
    27241 cacgctcatt gaaagcgaac tgttcggaca tgagaaaggc gcgttcaccg gcgcgctgca
    27301 gacgcgccgc ggacgcttcg aagaagccaa tggcggcacg atctttctgg acgaagtggg
    27361 cgaactgtcc gccgccgcgc aggtaaagct tctgcgcgtt ctgcaggaaa aacgatttca
    27421 gccgctgggc tcgtcgaggg tggtgaacgt caacgtgagg atcatcgccg ccacgaaccg
    27481 catcctggag caggatattc tcgccgaccg tttccgggcg gacctgtttt accgcctcaa
    27541 tgttttcccc gtttatctgc cgccgcttcg ggaacgcggc agcgacgtca tcctgctggc
    27601 cgatcatttt ctgctcaagt acagcaagga aatggggaag aacgtcaaaa agatctccac
    27661 ggccgccatt gaagcgtttc tgtcccacaa gtggccgggc aatgtccgcg aactggaaaa
    27721 ctgcatcgag cgggcggtgc tcctgaccaa aacggacacc gtggactgcc tgcatctgcc
    27781 gccttccctc catgccgggg aaagatcgcc ggagcgcagg gaccacagca agctgtcgtc
    27841 cgtcgtcgag gcgcaggaga gagcgctgat tatcgacgcg ctcagggaaa cgggcggcaa
    27901 ccagaccaag gcggccaaaa tcctgggaac cacgaagcgc gtgatccagt ataaaatatc
    27961 gaaaatgggc atcgatcccg gaaaattccg caaaaacaca agagacgccg ccttgaagcc
    28021 atagggcttt tgcggctttc cgcgcgccgg aaattcatcc atggaacaat aatgtcacgc
    28081 catttcgcaa aagcacaaat ttgttgattt ttatcccgca tcccgcggcg tcttccgcgg
    28141 caagcctata taatccatta taatttttgt ttgtttttca tttgatcggc actggcacga
    28201 ctctggcagt tgagccctcg ggttgaaagg ttcaaaggag agactatgtg ccgtttattt
    28261 gccgtaacat ccagtcagcc gcaatcgccg gtgatggcca tcaacgcgct caatgtcatg
    28321 aaagaagggc atgacggctc cggcgtgggg ctctttctga ccgatctggg cggcgagttc
    28381 gaaaaattca aaaacgagcc gatcctgtcc ggcatctttt ccaatgaagg catcaaggcc
    28441 ctggaccact tcatgatcga tcaggatttc atggtcaaat acaagctgtc ccttcggccg
    28501 accaaaccgg cgccttcggg aacgccgaag agagacaact acgtcattcg ggtttacgaa
    28561 tacccggccg aatgggaaaa cttaagccgg gaggaaatcg aataccgcct gatgatggtt
    28621 cagctccagc ttcggcagat cggcgaaaaa gatcaatcca tgctgatctt cagcttctgg
    28681 ccggacgtga tcatgatcaa ggaagtggga gatccgctgg ccgtcgccga gtatctgggt
    28741 ctggaccgca gggatctcac cgcccgggtg atcctgtgcc aggggcgcca gaacaccaac
    28801 tactccatca acatttacgc ctgccacccg tttttcatcc agggcatggc gacgatgacc
    28861 aacggcgaga acaccgcgtt tgtgccgatc cgggaatttc tgatgtcgcg caatttcccc
    28921 ggctacaccg gctaccagtc cgactcggaa gtgttcacac acatcctgca ttacacgcag
    28981 aacaaactcg gactgggcat ggaaatgtac aagcacgtga tcactccgct cagggacgag
    29041 gagctggcgc gtcatcccga cgggcggatg ctcagaaacc tgaagcagag ctgccggcct
    29101 ctgatcatcg acgggcccaa ctgcgtcatc ggctgtctgc cggacaagtc gatgttcatg
    29161 gtgcaggacg ccaagaaact tcgccccggc gtggtgggcg gccgtcccgg cattttcgcg
    29221 ttttcctcgg aaatgtgcgg cctggatgcc gccattccgg agcgcgacat caacctggac
    29281 gatcagccga tgagatatga aaccgtcatt gtccgccgcg ggcggcagga gatagaaaaa
    29341 tggaaccagt gggacacatt gccccatcta cactaagcgc caaagacctg ccctggcaga
    29401 ttgcctggca aaaggatcgg tgtaccctgt gcggccgttg cacaaccgtc tgcccggtgc
    29461 gggccatcga actgggcgtc caccgcaaac gcgcgctcac gacgccggtc ggtctggaga
    29521 gccgtcccgc caatctgttt tccgtgtacc acggcatcga tcagcgcacc gatccggccc
    29581 atgcctgcgt gggctgcggc atgtgtacgc tggtctgccc caacaacgcc atcgtccccc
    29641 tgcgcgccga tgaaaccgac aaactgcgct ttcacgtcaa ccagggcggc gaagcgaggc
    29701 gacggggcgg ccggagaaac gctccggaaa gcgtgctcga ccagatcaag tttgtccgca
    29761 tttccatgct gaccgacccg gcgctcgacg ccggacgaca tgaatttgag ctgcggaccc
    29821 tgctgggaag aatcctgccg ccggagcgga tgctccaggc cggccgtgaa aacggctgga
    29881 tgccgcccgt tcgcgagatt tatccgatga tcatcggcgg catgtcgttt ggcgcgctct
    29941 ccccgaacat gtgggaaggt ctgcagatgg gcgtggcgta tctgaacgag gagatgggca
    30001 tgccggtccg tatctgcacc ggcgagggcg gctgcccgcc ccggctgctc aagtcgcgct
    30061 ttctgaaata cgtcatcctg cagatcgcca gcggctattt cggctgggat gaaatcatcc
    30121 acaacctgcc gcagatgaag gaagacccct gcgccattga aatcaagtac ggacaggggg
    30181 cgaagcccgg tgacggcggt cttttgatgt ggtacaaggt caaccggctg attgccgcga
    30241 ttcgcggcgt cccgcagggc atcagccttc ccagcccgcc gacgcaccag accaagtatt
    30301 cgattgagga agccgtggcc aagatgatcc agtccatgtc catggcctgg ggattccgcg
    30361 tgccggtgta tccgaaaatc tcggcaacca gcaccacgct ggccgtgctc aacaatctga
    30421 cccgcaatcc gtacgcggcc ggactcgcca tcgacggcga ggacggaggc acgggcgcgg
    30481 cctacaatgt ttccatgaac cacatgggac accccatcgc cagcaacctg cgcgacgcct
    30541 acctgacgct cgtgaagctg ggcaaacaaa acgagctccc tctctttgcc ggcggcggca
    30601 tcgggaaaaa cggcaatctg gccgccaatg ccgccgccct gatcatgctg ggcgccagcg
    30661 gtgttcagac gggcaaatac atcatgcagg cggccgccgg ctgtctggga tcggagggcg
    30721 accgctgcaa tgtctgcaac gtcggcgttt gccccaaagg catcacctcg caggacccgc
    30781 ggctggatcg gcggctggac ccggagcggg ttgcccagcg gctggtggac gtctatttaa
    30841 cgttcgacac ggaactgaaa aagatcgtgg cgccgctggg gcgctccact tcactgccga
    30901 tcggcatgtc ggatgccctg ggcatcagcg atgcggacgc cgccgggcgg ttgaacatca
    30961 aatacgttct gtagtgggga acggttttaa accgttccct acggtaacgc cacggaattg
    31021 gaaaacaggg taagacgatg accagaaaag atacgatcaa aattgccggc gtggaaaaca
    31081 ccgtccgcgt ggattcacgg attctggaag aacgcattca gaaagccgtg gcagacggtt
    31141 gccgtcatat tgaagtcacc gccctggggc agcacggcat tggcggccgt ctctggcggg
    31201 cgcacgacga accggtgaag gtcgatgtgc tgggcacgtc cggtcagcgc ctggggtcca
    31261 tgggatttgc gaacaccacg attcaagcat tcggccccgc ctctgacgac gtcggctggc
    31321 tcaacgcggg ggcaaaaatc attgtgcacg gaaatgccac caacggcgcg gcaaacgcca
    31381 tggcgcaggg acgggtgtac atcggcggca acatcggcgc gcgcggcatg accatgacca
    31441 aatacaaccc gcgctttgag ccgcccgaac tatgggtttt cggcaaagcg ggagactcgt
    31501 ttgcggaatt catggcgggc ggcatcgccg tgatttgcgg cgcgggcgcg gaagaagaaa
    31561 aaaacgtgct cggataccgg ccctgcgtcg gcatggtcgg cggaaaaata ttcttccgcg
    31621 gcagtcagga agcttacagc caacccgatg cccggctggt gaccgacctt ccggacgacg
    31681 aatgggagtg gttgaagcaa aacctcaaaa catttctcga tgccatcggc aggcagtatt
    31741 tatttgaaca gctcgcggaa aaacgggaag actggcagct tttgatcgcc cgcaaacctt
    31801 ctgaaaaggc ctctcgattc gttcgtccga tggcgaaatt caaacaggag gtctgggaac
    31861 atgagctggg ccggggcggt ttgatcggcg atttgtcgag tctggaccgt tctccgatcg
    31921 acgtgattac caccggagcg ctccggcgct ttgttccctc ctgggaaaac gaaaaatatc
    31981 ttccgccctg ccaggcggcc tgcccgaccg gcattcccgt tcaaaaacga tgggaactga
    32041 tccgcaaggg ccacctggag gaagccgtga atctggcgct ggagtacacc ccctttcccg
    32101 cggccgtctg cggctatctg tgtccgaacc tctgtatgca gaactgcacg cgcagcctgg
    32161 tccatctgga gccggttgac gttaccctgc tgggaaaagc gtctctggaa gcgaaggaac
    32221 cccggccccg cccgcagacc ggaaaaaaag tggcggtggt cggcggcgga gcggcgggcc
    32281 tttccgtcgc ctggcagctc tggctgaaag gccatgaaac cgtcattatt gagagccaaa
    32341 agcggctggg agggaaaata gcggatgcca ttccgcaaag ccgcattcct tccgacgtgg
    32401 tggagcatga aatcgggcgg ctggccaaaa gtattcacaa ggttcgcctg ggcaagcctt
    32461 tgacgaaaga gcagttcgcc aggttcaaaa gcgaacatga ttttgtcgtg attgccacgg
    32521 gcgcgtcgcg gccgcgcgtc ctcaacattc ccggcgtgga aaaatccgta agcgcgctgg
    32581 agtttttgcg gcgaagcaaa ctggactgcg ccaaggtcgg tgaaaaagtc gtgattatcg
    32641 gcgcgggcaa tgtgggctgc gatgcggcgt cggaagcctt tcgcctgggc gccaaaagcg
    32701 tcattctgat tgacgtccag gaaccggcct ccttcggcgc ggagagaaaa caggccgaag
    32761 ccgcaggcgc aaaatttctc tggccgcgct tcaccagggc catcacggac cagggcatcg
    32821 aattgaccgg cggcgagatg ttgccggcgg aaaccgtcat cgtcgccgtc ggggaccttc
    32881 cggatttgtc ctttctgccg cagagcatcg cgacggaaaa ggggtttgtc gtggtggata
    32941 aaacatacgc cacgagcgat ccgcaggttt acgccatcgg cgacgcggtc cgtcccgggc
    33001 tgctgaccga cgccatcggc gccggacgca ttgccgcccg gtcgatcgac gaccggctgc
    33061 ggggggccgc cgagacctac gacaagctgc cgccgatcgg ccaggaacgc gttaaactgc
    33121 agtatttcga cccgcggatt cgcgcgtttg ccgacacggc ttcctgcgcg acgaactgcg
    33181 cctcctgcgg ggcatgccgc gactgcggcc tgtgcgagga aatatgcccg caaaacgcga
    33241 tctttcgcaa aacactgccg gacggcggat ttgaatatgc cgtcgacggc gagagctgca
    33301 tcggctgcgg tttttgcgcg ggcgcctgtc cgacgggagt ctgggaactt tcggaaaaca
    33361 agcctttgga ataaaaaact ttttagctaa ggataaaaaa gacatgaaaa agacaaaagc
    33421 aacagcggcg gcggaaagac attacgtgat cgaaaaagag aagttcgttc ccgtttcgca
    33481 gtatttcggc gaggacacct tcaaccataa ggtcatgaag cagaagcttc ccaaagacac
    33541 ctttcgaaaa ctgatggaag cgatgaacga agataaaaag ctggatcacg aaacggccaa
    33601 catcgtcgcc cacgccatga aggagtgggc gctggaaaag ggcgcgaccc actttgcgca
    33661 ctggtttcag cccatgacgg gcatcaccgc cgaaaagcac gacgcctttg ccgatccctg
    33721 cggccccggc gctgtgatcg agcggttttc cggcaagcag ctggtgcagg gggagccgga
    33781 cgcatcgagt ttcccctccg gcggcattcg ggcgaccttt gaggcccgcg gctacacggc
    33841 gtgggacatg tcttcgcccg ccttcatcaa gcgcaacgga atctccacga cgctttgcat
    33901 tccgaccgcc ttcatttcct acacgggaga agcgctggat aaaaagacgc ctcttttgcg
    33961 ctccagccgt gcggtcagcc aaagcgccgt gaacatgctc aagcttctgg gggccaaaaa
    34021 cgtccggaga gtttactcca atctggggcc ggagcaggag tattttctga tcgacatgga
    34081 ttactactac aagcgtcagg atctggtgct gggcggccgg acggtggtcg gcgcgccgcc
    34141 ggccaagggt caggagctgg aagatcagta tttcggcagc attcgcgaga ggatttcctc
    34201 ctacatgcat gacgtggaag aggacctttt caaactgggc attccggcca agaccaggca
    34261 taacgaggtc gcgcccagcc agtacgaaat cgcgcccgtg tttgaagagg ccaatctggc
    34321 ggtcgatcat aatcaactgg tgatggatac gctgaagtcc gtggccagaa aacactattt
    34381 atccgcgctg ctgcatgaga agccgtttgc ccgaatcaac ggatcgggga aacatgtcaa
    34441 ctggtcgctt gccgacgaca acggcaacaa tctgctcaac cccggcaaaa cgccccagga
    34501 caacatttcg tttctggtgt ttctgatcgc tgcggtccga gccgtttaca ggcacgccga
    34561 tattctgcgc gcctccgtcg cgtcctacgc aaacgaccac cggctgggcg ccaatgaagc
    34621 gccccccgcg attatttccg tcttcctggg agaacagctg acgcagatcc tcgacagcat
    34681 cgaaaaggga aaagtcgcga aggcaaccaa cgaggaaatc atcgatctgg gaatttcctc
    34741 tctgccgcag gtcagcaagg acaataccga ccgcaaccgc acttcgccgt ttgcctttac
    34801 cggcaacaag tttgaattcc gcgcggtggg ttcgtctcaa tccatttcgt ttccggcggc
    34861 ggtcatcaat accatcgtgg cggaaagcct ggacgacctg gccgaaaaga tcagggccaa
    34921 aggatcgaaa aacatcaatc aggcggtctt tgaggttttg aaaagcgaga ttcgggtcat
    34981 caagccgatt ctgttcaacg gcgacaacta taccaaagcg tgggaagccg aagccggcaa
    35041 acggagtctg ccgaatttca agacaacgcc ggcggcgctg ggggcgctgg tcacggacaa
    35101 ggcgcttcgc ctttttgaga aataccgggt cctcacccgg gtggaacttc agtcccggta
    35161 tatcatttat ctggaaaaat acatcaagga cctggaaatt gaagtcaagt gtctgggcaa
    35221 tatatgcctg aaccaggtca ttccggcggc ggcggactac cagaagaggg tggccgaagc
    35281 cgtgatcgcg accaaagcgg cgctgggcgg cgcggcggtt gccgctgaag ccgacctctt
    35341 aaagaaagtc atccgactgg tcaacaccat ctacgccgga cacaaggaaa tcatggccgg
    35401 gatggaggcg gcaaacgccg gccatgatga agcgaaaaag gcggaaaccc tctgcgaaaa
    35461 aatcaaaccg agaatggatg cgctgcggga atccgtggat gaactggaaa acctggtgga
    35521 tgacgagaga tggcctctgc ccaagttctg ggaaatgttg tttatctgct gacacaaagg
    35581 cagggaacgc ctggacggtt ttccctgccg ccctctttga gggaccatgg aattgcaaaa
    35641 ggacccatcg tgaaaaatac gaaatttatt tttgtgaccg gcggcgttct gtcgtcgctg
    35701 ggcaaaggcc tggcggcggc atcgattgcg gccgtcctgg agtcgcgcgg tcttcgcgtc
    35761 accaatcaga agctggatcc ctacatcaac gtggatcccg gcacaatgag cccgtttcag
    35821 cacggagagg tcttcgtcac ggacgacggc gccgaaacgg atttggatct ggggcattac
    35881 gaaagatttt cctcaacctg tatgggcaag cgcaacaacc tgacgaccgg gcaagtttac
    35941 ttctccgtca ttaccaaaga aagacgcggc gattacctgg gcaaaaccgt tcaggtcatc
    36001 ccgcatatca cgaacgaaat caaggatttc atccgcgaaa ccgcgaaagg gttcgacgtt
    36061 tccatcgtgg aaatcggcgg aaccgtaggc gacatcgaaa gccttccctt cctggaagcc
    36121 atccgccagt tccgcaacga agtgggccgg caaaacgcca ttttcatcca tctgacctgg
    36181 gtgccgttca ttaaaaccgc cggggaagtg aagaccaaac ccacgcagca cagcgtgaag
    36241 gctttgcggg aaatcggcat tcagccggat attctgttgt gccggacgga aaactttctg
    36301 tccgacgaga ttaaaggcaa gattgctctg ttttgcaatg ttgaagtcaa ctgcgtcttt
    36361 acggccaagg atgtcggctg catttacgag tgtccgctgg tgtatcaccg cgaaggactc
    36421 gatgcaaaga ttattgaact gctcaacatg tggaccggac aaccgaagct taccgcctgg
    36481 gagaatgtgg tcgaaaaatt ttatcatccg gcccatgaag tctgcatcgg catcgcgggc
    36541 aaatacgtca acctcacgga ttcctacaaa agcctgaatg aggcgctcgt gcacggcggc
    36601 atcgccaaca actgccgggt gaagctgaaa ttcgtcgata cggaaaaaat cgagcaggaa
    36661 ggcgcggcga atcatctgtc gaatgtggac gccgtgctgg tgccgggcgg cttcggcaac
    36721 cgcggcatcg aaggcatgat cctggcggcg cgctacgcgc gggaaaacca aatcccgtat
    36781 ttcgggatct gcctgggaat gcagatggcg gtgatcgaat acgcccgtga tgtctgcggc
    36841 cttaccggcg cgaacagctc cgagtttgat ccggcgaccc cgcatccggt cattgacctg
    36901 ctgccggaac agcgcaccgt gagggacaag ggcgcgtcca tgaggctggg cgcatggcca
    36961 tgttccattg ccgagtcctc ctttgccttt gacgcttacg gccaaagtga aatctcggag
    37021 aggcaccgcc accgctatga attcaacaat gactttaaaa aga
//
LOCUS       DAOPAF010000011        36757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1290_length_36757_cov_64.943791, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000011 DAOPAF010000000
VERSION     DAOPAF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 36757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36757
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_36757_cov_64.943791"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLQ77_02290"
     CDS             complement(<1..559)
                     /locus_tag="PLQ77_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPL09540.1"
                     /translation="MGKLFGTDGIRGEANQYPMTPEIAFGVGRAMAHLFKKNGHRAVI
                     IIGKDTRLSGYMLESSLEAGITSMGGYSYLVGVMPTPAIAFVTQSMRADAGVVISASH
                     NPFQDNGLKIFGGDGYKLSDEQEEFIEDLILNNKLSDLVSRGQDIGKAYRLDGVNGRY
                     IVFAKNTFPRNLSMEGMKIVLDTANG"
     gene            complement(621..1862)
                     /locus_tag="PLQ77_02295"
     CDS             complement(621..1862)
                     /locus_tag="PLQ77_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPL09541.1"
                     /translation="MDFKPRSHEKIQQLLDKGVNIPNPATLDIGDDVKIDQISGKGTA
                     IYPGCRIYGGKTVISSGCKLGYEAPATIEDCQIGERVELKGGFFSKSVFLEKANLGMG
                     AHVREGCLLEEEAGGAHSVGLKQTILFPFVTLGSLINFCDCLMAGGTSRSNHSEVGSS
                     YIHFNFTPDADKTTPSLLGDVPRGVMLNQPPIFLGGQGGIIGPVRMGFGNVAAAGSVL
                     RHDYPDDGLLIFETTPADGAKNYRRAAYPNLRRIVENNILYLANLSALESWYAHVRRP
                     FLDARPFGPLIYAGVLETLASAKKERLKRLKAMAQKAAANPGPEEEARKEFLEQTGFC
                     EDLFTGKIPDGFTKTDPLLEAFLQDFEKSAGNDRTDYVGAVRKLPGAVSAKGTAWLNG
                     IVGALCNQAARAMPSMGLFRN"
     gene            complement(2029..3390)
                     /gene="gdhA"
                     /locus_tag="PLQ77_02300"
     CDS             complement(2029..3390)
                     /gene="gdhA"
                     /locus_tag="PLQ77_02300"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPL09542.1"
                     /translation="MSVIQEVIEKVKQTDPDQPEFHQAVAEVMETLEPTVKKHPEFVK
                     ASIYERIVEPERAVIFRVPWMDDQGKVRVNRGFRVQFNNAIGPFKGGIRFHPSVNLGI
                     IKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDAEVMKFCQSFMRELYRHIGADVD
                     VPAGDIGVGAREIGYMYGYYKKIRNEHTGVLTGKGLDYGGSLIRPEATGYGTAYFAAE
                     MLATRGLDFKGKTVAISGAGNVAQFAVEKVNQLGGKVISLCDSSSCIIDDAGIDMDKC
                     SYVLDLKNVKRGRISEYADKYKQSTTCFEGKNVWDVIREQGIKVDIALPCATQNEING
                     KNAAALVKNGCYCVAEGANMPSTPEAIKIYQENKILYGPGKAANAGGVATSGLEMSQN
                     SLRLSWTREEVDNRLLIIMKSIHKNCFDTAQAYGKKGDYVTGANIAGFTKVAKAMLAY
                     GVV"
     gene            complement(3424..4425)
                     /locus_tag="PLQ77_02305"
     CDS             complement(3424..4425)
                     /locus_tag="PLQ77_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPL09543.1"
                     /translation="MKELFKPEDIERKVLLILKILHESPDPMGARVIARRMSDYHVQL
                     SERTVRYHLKIMDERGLTKLVGRRDGRVITEQGFAEISDARVQDKIGLSISRIDLLSF
                     RTTFDVKKKRGLVPVNVSFFPKEHFSRALSIMKPVFQQKLAVSSRVAVAQAGKSLGDI
                     AVPEGSVGFATVCSIVINGVLLKHGIPIDSKFGGILQMRGGKILRFVELIHYAGSSLD
                     PSEIFIRGKMTSVRSVARTGEGKILANFREIPALSLPLVERVMEDFAGAGLHGVLSIG
                     HIGHPVCQTHVDMNKIGMILIGGLNPVAAVCEEGLTVDNKAMSTVMEFEKMRDIETI"
     gene            complement(4552..8007)
                     /locus_tag="PLQ77_02310"
     CDS             complement(4552..8007)
                     /locus_tag="PLQ77_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPL09544.1"
                     /translation="MNGPETIQSLADCVNRLKREDVQECLFFQKDRTADLTRVAGGMK
                     AFLAAEEKILEDRAAGLSSAGLETVNRRLLSLKDICWMLESDILANLQKVLSLSGAEK
                     YSSLQPAAFRKYRKLNLLLNALDRLEVRGRDSAGIQLAFFLKDAKVLEELDRRIEENG
                     WGEDYRRRTQKGDLINRSIFISGGAQNPGGGIHIAFTYKTFSVVGELGRNVADLRQWI
                     SRDAILQWFAARASADESAIAHTRWASVGSITEENCHPVNNATLRPELSDFPAYPGRE
                     AHISVVLNGDIDNYPALRRDLEQDGEQIAPDVSTDTKIIPLQIEKYLRKGQNLTDAFR
                     LAVNDFEGSHAIAMQSNLEPGKIFLALQGNGQSIYVGVGEDAYMISSELYGLVEIAPR
                     FIRMNGEEAGGQLFILDQNRGTGAKGIAACCYDGAPIHLEERNLQTAEITTRDIDRGC
                     FPHFFLKEISESVRSVQGTLRGKYRITGAASAPSGILFNLGEDMVPASIRNGLQSGLT
                     RNIFVIGHGTAAVAGQAVADALAHCLGMSRLNIQSRVASEMSGFGLRDDLSDTLVIPI
                     TQSGTTTDTNRAVSMARARGAQILSIVNRRQSDITASSHGVFYTSDGRDIEMSVASTK
                     AFYAQLVAGQLLALYFAKLLGTMSDEAIVRRIRHLENIPQAMQRLFDRRDEIAACVTQ
                     GAGKRYWAVVGSGPNKAAADEIRIKLSEMCYKTISSDIVENKKHIDLSAEPLILVCAA
                     GNPDVVLDDIAKDIAIFRAHKAATVVFADEGDLRFHPAADAVIAVPKTPAPLSVILNT
                     MAGHLWGYYAACAIDGEAQLFRAFRGRLALGRLQKKKTKLSVLDVFDLMADPSFRRII
                     NDFCSRFQSRLQGGALSALGGKTIADLTLLLKYASGKLPLQDIRQDFPGHEDLVQPFD
                     LLDETLGAAIDELTRPIDAIRHQAKTVTVGTSRKEKEPQGVVFDLLGALNFSVKNLTW
                     RNIRTIHRLQPSVGAVEGYTLYRVRGLDEKGNPGPASTIAVCAKGGAALRMSSRAEQP
                     TTLMGVKKTIVSTGHAYLGRGKIDSASIMVIPLQETDGRIGNLLLIHIAFNERLSGPE
                     KIEVLGHRYDDIRNLINEYNLAWNDRYMEALPLECLFGESVEEIARRIQARCNPSPVK
                     NTAFT"
     gene            complement(8698..9000)
                     /locus_tag="PLQ77_02315"
     CDS             complement(8698..9000)
                     /locus_tag="PLQ77_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL09545.1"
                     /translation="MKAKKQINPAAGIDVQEALNKAVEEILEKKLDDLATVLSGSDNG
                     RSKLHAEVMAMVETGLFKIALRRSGNVKSSAAAFLGIHRNTFTDKMAKLGLDGEKE"
     gene            complement(9082..9594)
                     /locus_tag="PLQ77_02320"
     CDS             complement(9082..9594)
                     /locus_tag="PLQ77_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09546.1"
                     /translation="MNAVYKIRKAFRYPFVTLTVLLFVLFLTGLARGECWEKILTGAL
                     FAAALVVAVESSEREFGISENGLRIRKFFRTRHFTWAEITHLGTVILRSKAYFLLTTT
                     KGFYLFSNLLQDHTRLLGQLAEKLEDERVETEIKNYIEAPKERTSLIVLTWVALVLLV
                     AVVLTRWLAA"
     gene            complement(9609..10094)
                     /gene="moaC"
                     /locus_tag="PLQ77_02325"
     CDS             complement(9609..10094)
                     /gene="moaC"
                     /locus_tag="PLQ77_02325"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPL09547.1"
                     /translation="MKKLSHINDEGQVRMVDVTAKTATHRQAKARGQVTMKPATMKLL
                     AEGRIAKGNVLTAAKIAGILAAKNTGGIIPLCHPLGLTGMDITFVLDRKKSRVVIESQ
                     VRTVGQTGVEMEALTAVSVAALTIYDMCKAVDREMVIGEILLLEKSGGKSGAFVRKAA
                     K"
     gene            10276..11805
                     /locus_tag="PLQ77_02330"
     CDS             10276..11805
                     /locus_tag="PLQ77_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPL09548.1"
                     /translation="MFIKVQSMSVIGMESYPVTVEVDVSRGLPHFATVGLPDASVKES
                     RDRIKAAIKNSGYAFPRDHVTVNLAPADIRKEGTGFDLPIAVGILAAEALIEEAALDG
                     CFFIGELSLDGSIKGVRGVLPAAFKARESGIRSVFVPEENAAEAAMVEGIQVYGVRTL
                     PDVVEFLADRKIIEPLSVNADRLFQVHRHYEIDFSEIKGQKQALRALEIAAAGSHNVL
                     MIGPPGSGKSMLARRLPTILPELSFEEAIEVTKIFSVAGLLNAGETLVAARPFRAPHH
                     TISDAGLVGGGQMPHPGEISLSHLGVLFLDELPEFKRNALEALRQPLEEGAITITRSA
                     VTAAFPARFMLVAAMNPCPCGYFGDRVRRCRCSPQQIRQYQAKISGPLLDRIDLHVEV
                     PSIQYRALSGKQENESSAHVRERVVRARRRQKERFGGAGIPTNASMTEKQIRSFCPID
                     EESHQLMEMAVDKLGLSARALDRILKVSRTIADLEGAAKIEAAHVAEAIQYRSLDRNI
                     V"
     gene            11865..12935
                     /locus_tag="PLQ77_02335"
     CDS             11865..12935
                     /locus_tag="PLQ77_02335"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPL09549.1"
                     /translation="MKIKIIKALPKYLKPKPDEKKLGFGQHFTDHMFTMKYREGDGWQ
                     DPVIEPYHYLQLDPTAMCLHYGQEIFEGLKAYRGANNEIYLFRPMENIRRMNVSAERL
                     CMPPMDETVVLNALRQFVVLEKEWIPREKGSSLYIRPTMIATEPALGVHPASEYLFFI
                     IAGPVGAYYAHGFGPTKIYVSDDCVRAAPGGTGHVKAAGNYAASLYASRIASTMGYTQ
                     VLWLDARENRYVEEVGTSNIFFLIEDRLITPPLGGTILPGVTRDSVIKLAAHLGVKVE
                     ESRLTITEVISAIENGTLRDAFATGTAAVISPVGQIFYHNREYLINDGKTGELAEKLY
                     NEILQIQYGTREDPFGWRMQID"
     gene            13246..13938
                     /locus_tag="PLQ77_02340"
     CDS             13246..13938
                     /locus_tag="PLQ77_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPL09550.1"
                     /translation="MKAKLNKIELFFPAGVLAFCLALFWAVVFPGASTAAPPLEEVVD
                     RLQRIYEKTQDFQAGFIQETTVKSIRKTDTESGTVYFKNPGQMLWDYKKPKAKKLIIN
                     AQKAWLYLPQDKTVYVQDSGKIMKSEALIKFLSGLGKLNNDFTIQYASPAEDPDGNCL
                     LLLYPREKGASYSFLRMTVAKNNFYILQVGFDDVMGNSTLLRFSNIKMNSGLSSKLFQ
                     FQPPPGVSIFKM"
     gene            14004..15020
                     /locus_tag="PLQ77_02345"
     CDS             14004..15020
                     /locus_tag="PLQ77_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL09551.1"
                     /translation="MPRGARIDAADAVHHIMARGIESGKIFDSDADRDNFLYRLGDIF
                     PDTATTCFAWSLMPNHFHLLCRTGNIPISTVMRRLLTGYATWFNRSRKRHGHLFQNRF
                     KSILCQEDAYLLELVRYIHLNPLRAGLVQNLNKLDSYPYSGHSALMGREHRSWQETQG
                     ILMLFGNTQDAARQSYRQFVGQGIEQGRRHDLTGGGLIRSAGGWEGLKRKCEEGTYQR
                     SDERILGDGDFVSQVLSKTEEKIKKSYALRNSGVDLDQVALRVSALTGVKPGDVRTAG
                     KQPHIVNARSLFCFWAVRDLGETMAGLARRLGLSIPAISKSVVRGRQIAESNGFELMQ
                     NNHN"
     gene            complement(15094..16014)
                     /locus_tag="PLQ77_02350"
     CDS             complement(15094..16014)
                     /locus_tag="PLQ77_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09552.1"
                     /translation="MFMIIPELLDMYRSCALCPRACHVDRTKGEPGYCRLPSDIVMDC
                     ALPHHGEEPPLSGTGGAGTIFLSSCNLGCVYCQNYQISHRAQGRRLTVIELARIMIDL
                     QKSGCHNVEPVTPTPQTPLVMEALCLARAQGLTVPLVYNCGGYENPDVIRMLDGMVDI
                     YLPDFKYGLEEDARMFSDAPDYSKFAVASLQEMVRQVGDDLDVENGIASRGVIIRHLV
                     LPGRLENSKEALRLLRGEVSARIPISLMSQYTPVPKVRSHPLLGRRVTASEYHEIVEF
                     ALRLGFENLFIQEVDDRALTPDFKREHPFN"
     gene            complement(16156..16923)
                     /locus_tag="PLQ77_02355"
     CDS             complement(16156..16923)
                     /locus_tag="PLQ77_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPL09553.1"
                     /translation="MDYKNIIVTTENFVTTIVLNRPPMNSVNLGIREEFFDAVTNIEK
                     SRETRAVIVTGAGEKGFSAGMDVSDVANITRGPNGNDIMNAISRSAKPYIAAINGHAL
                     GGGCETAIACHFRIMTDNPKALIGLPEVNLGITPGWGGIQRLPRLLGKSKALELILFS
                     KRLPPTEALAVGLVDKVVPAADLMKEATAFAQALTKRPPLAVAAVLEGISVGMEKGFN
                     EGLKLDIEWSNKLAASKDAVEGITAFLQKREPNFTGE"
     gene            complement(17031..17912)
                     /locus_tag="PLQ77_02360"
     CDS             complement(17031..17912)
                     /locus_tag="PLQ77_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPL09554.1"
                     /translation="MKIDLRQIQSLGSGLTRPEGVMALDDGGVRTADAHGRCARIDPD
                     GRTTFFGQLGGLPNGICIDTDGNCIVANIGNGEVQSLSPDGKHAVLMTEAQGKKMSTP
                     NFPFLDFAGRLWVSNSTDNPDIDASLKSPVPDGCLVLIANGRTPRIVAEGICFANGVA
                     VDADEQYVYVAETMKRRILRYRIHADDTVGPAEVYGPEFLGKLGFPDGIAFDEAGNLW
                     IAFPVANAVGCIDPRGGLEICADDPQGEVLNRPANICFGGKDRRTAFIGSLGGANVPF
                     FEVPHSGQRLVHQKSRP"
     gene            18154..18906
                     /locus_tag="PLQ77_02365"
     CDS             18154..18906
                     /locus_tag="PLQ77_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL09555.1"
                     /translation="MFNRIVSVVFLVFIALTSIPLFLTALLLRLATYPFDRRLRLLHL
                     FTCFWASLYTWTMPAWRIYIEGREKIRKNATYMVVSNHQSQLDILVAFRLFFHYKWVS
                     KIEIFRVPLIGWNMRLNRYVQLKRGDKQSIEKMLRDCAVHLEEGSSIFMFPEGTRSPD
                     GEVKAFKPGAFQLALQKNVPILPIVISGTNKALPKYSMNFNGVQKIYVKVLEEIPYEA
                     FAHLSVAQTAEMVHGFITRQLRLMKECTPDPA"
     gene            complement(18931..20052)
                     /locus_tag="PLQ77_02370"
     CDS             complement(18931..20052)
                     /locus_tag="PLQ77_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL09556.1"
                     /translation="MKQPDKPFDMPALAADFAARHVACRADLQTEQEFPSDLWRKMGK
                     AGLFRGGLPEEYGGDGCGYLDLLRTGEAFVRSGLNLGLGMSWIFQQIIARSVVGTFGT
                     TEQCRQYLRAAALGNCAISFAVSEPGRGASPKALATKAQQQGESYLLTGEKTHLTNGP
                     IADLFVVVAVTDDTSARKGFTAFMVPRGAQGLFVEPPMPLNFLKPSPHGGIQLNGCLI
                     PKASVLGREGNAWEDIVVPLGETEDVVMMGPVLGGMAAQLDLLRIALRERSASAEKTL
                     PGEWGALNSLWQALRSIACEAAGRLDRGNESPAPLSIAFARFAALFHADVARFTERLR
                     IEEPERFSLLQKDMEFMEMLQKRRLQIRQAKMGAAQLAS"
     gene            complement(20049..21434)
                     /locus_tag="PLQ77_02375"
     CDS             complement(20049..21434)
                     /locus_tag="PLQ77_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPL09557.1"
                     /translation="MVKLIGQFVSCCRAAGLRVSTSEVLDTLKQLELIDPTDELQFKA
                     LLRANFAKSLRELKHFERLYHLFFHEMRVNAEDMQQAASQIDRIREATDLLRKKPHDN
                     PMYDAVVDFLAGNPMALLAEMRRIHLESDDAAAPARFNLGPLASRFNVLLQINAAANA
                     AAALLENNELNMTPADRHNLAAHFEERVEAARSILVYEPQAGDGGAKKVKTHEQRVRD
                     LGEKAFSSFTREEVEEMREAIDRLVRKLKNIVSRRFAVRDRGNLDVKKTLRASAKYDG
                     VPLDIKYRRKSLRKSRIVTLCDVSGSVWAAARFMLNLVYSLQDCFDKVNSFVFIDQLT
                     NVTGIFEEQDISEAIRIVMEEADVHYSAPTDYGETLRNFKRDYMDLLTKKTTLIIVGD
                     GRSNYMNPEDAILGAMRERCRRVIWLNPEPEKLWGTGDSEIRTYLRHCHEVRACRNVN
                     QLFTFIEELVI"
     gene            complement(21559..22476)
                     /locus_tag="PLQ77_02380"
     CDS             complement(21559..22476)
                     /locus_tag="PLQ77_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPL09558.1"
                     /translation="MSYKTVSDVEKRLIEAGYIPSREIATVIFLAQATQKPVLIEGPA
                     GVGKTELAKAVARSLGRELIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKDRINDIIG
                     ATATLAEAVDAIAAQEDAFFSDRFIQPRPLLQAISSAKPVVLLLDEVDKSDPEFEAFL
                     LELLSDFQVSIPELGTRTAVRIPFVLLTSNNYRDMSDALKRRCVHLYIDYPARDTEIS
                     IVRLKIPDIEERLCAVLVDSIRKIRTLDLKKSPSISETLDWAQSLLALQITELTPEVI
                     SETLNVICKYQADLEKVRKNIDRVVAGAG"
     gene            22703..23956
                     /locus_tag="PLQ77_02385"
     CDS             22703..23956
                     /locus_tag="PLQ77_02385"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPL09559.1"
                     /translation="MTWEAMANIFQGITTLTESFSHNPRMAAARIGLILLGFLMIYLG
                     KKGYLEALLMIPMGLGMATVNAAIMFFDPLNLHGGVGTLFVEAQAGATADAVKNAAEI
                     VTILQIDWLQPIYTFTFSNGLIACLVFMGVGVLLDVGFVMARPFQSMFIAVCAELGTI
                     VVFPIAQWFGYTPQQAAAISIVGGADGPMVLFTSLRLAPELFVPIAIIAYLYLGFAYG
                     GYPYLIRLLVPKKLLGIKPAPAKKKRSITSGEKMLFAVVACVFLSFLLPVAAPLIFSL
                     FFGVVIRESGIKEFIDLLQGPILYGSTMFLGLMLGVLCDANTIMDPKVLPLLLLGILA
                     LLISGIGGILAGYVLYFMTRGKFNPAIGIAGVSCVPTTAKVAQKVVSKVNRGAIILPD
                     ALGANVSGVITTAIIAGIFCSLLAK"
     gene            23969..24094
                     /locus_tag="PLQ77_02390"
     CDS             23969..24094
                     /locus_tag="PLQ77_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09560.1"
                     /translation="MSGWPEALLTYGLTIVIAFGIAGMIHLMVVLMEKFPPKKDN"
     gene            24188..28426
                     /locus_tag="PLQ77_02395"
     CDS             24188..28426
                     /locus_tag="PLQ77_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPL09561.1"
                     /translation="MTTKSPSMEKSDSWVSSALKVNLERTAAEVQIPLQYAPLLEIVK
                     DHYGLRKKTSELLTELNHPYVNWDFVLRELKSISIGNFHLYSRHADGLAGLWILLTIY
                     FDILKSAAGEDVKDSALHYLFDYADTVLLHSEEFLERNLPLFPEMIESLAAMAKGDPA
                     LFRKCSSYLKRIIRTAAEKNVDLATPAFDALLYKMFRATYAFWLSQPDPALWLIAERR
                     GDAPPNETPYLELIRPLSHHRFRRLMLELEALRPREGEINGARIRDFLALPDYFQILD
                     TYLRVASALEKTEAYAGRHLVKLDFLFGIMTAPGLRDIHAGAMREINASLRLVFQEEK
                     KENLDIFVRKIFGFLKRNTSRGEFRSAGIDCILTAAREVFAQNDHPLVEAFMDELIAY
                     GFEYPEIQGATAEWQVRVNPEHVRMIRAWLEMIALKPRWTKRLISALIIHLKTGGIFI
                     RDTDLIQRDISRLLNTDIAPAYSLIKQLLRLFPVYFSEIGAEGELRDLTTRVDELSCR
                     NDRLVDFFRKQSHVESNSLLVEFIEDMFRFWVSGDKKSIRKHVPDEIFDQVAGEGPYF
                     DGMHRALLYLFPLVGNQPQNFLEWDPERIAAEISRIQNVSETDRERASLMVRIYQLIY
                     KKYHPRYHDLLTDLETAGVFAAPDLSSLKRSLAEKHHYRSLTLILKCLGSLKARILSG
                     KKTRSFENIYYKRHIAAGIPSMYGTYHEEKFDALGLSLRLESLAGMLFEELIQSLNLQ
                     FITKRTIIRIHTYLWTYLTALDLEGISTEGLVAKVRFITSALPIKQFSMEQYLDIFRF
                     ISRGIQDVIRDYYIDAHSVNLPVILRQLQARADQTGAAAGGALPDQFIYQQSENFLRG
                     VISSAFGLQVLDNFVHTVIKTLNAELEKFKDNKRIVNLLMDYNPDLAVSPLYGRNPKM
                     DNQILLGNKGYFLKKLVSFGFQVPPGFIITTEVFRGFDAVYGYKYIFRDLARRVNREI
                     AALERKTGRRFGDPVHPLLLSVRSGATVSLPGMMRSFLNVGINAAIAENLSARKDFQW
                     AAWDSYRRFLQTWGMFQGLSRDFFDAIMDGFKQKHGVQRKIQFPPERMRQIALAYREG
                     ILDSGIPLSDNPLRQLRHAVLQVFDSWYAEQARIYRHQMHLSDAWGTAVIVQAMVFGN
                     LHERSGSGVIFTRDPKSASSDVALYGDFIFGVQGDDIVSGLVETYPISERQRLAEQRN
                     TGISLEADFPAIYAELVKTAETLIYEKGLNHQEIEFTFEGPEKEQLFLLQTRDMDQTK
                     AKSLRRFRETDLLHASMLGTGIGVSGGALCGRAVYSEKDIQQFRAREPKTPLILIRPD
                     TVPDDVGVLLQVEGLLTAKGGATSHAAVTIPQLGKVGVVGLSKLKVYEMEGYATLEGR
                     TIQSGDFIGIDGWSGVLYLGKHDLESTPSATLTL"
     gene            complement(28416..29402)
                     /locus_tag="PLQ77_02400"
     CDS             complement(28416..29402)
                     /locus_tag="PLQ77_02400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPL09562.1"
                     /translation="MPRNGSFEIQCSLEKALAENRNLKLQFDATVRQVHELNSFIRLL
                     LNMSPFGICIVQGGRLIFSNKTFCDICGCSSSELRGIDPLPMVFPEDRDHVQNSVRSS
                     LEEQVDSFFIFRVLTRKDAIKWILGSVNVIHMNGKRAFLGNFVDLTEGRVMQLAYSDP
                     LTGLPNRKLMWDRLEQAMVSAKRRSCRLALLFVDLDKFKDINDAYGHKTGDQLLAQVA
                     DRLRSAVRRENDTLARIGGDEFLILLTDVSQRTDIENFIRLLFEKFRPCLQVGSPPLE
                     VQVGFSVGVALYPEHGESADVLIHHADLAMYQVKKARGEEKFCFFDAQSPTA"
     gene            complement(29611..30882)
                     /locus_tag="PLQ77_02405"
     CDS             complement(29611..30882)
                     /locus_tag="PLQ77_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPL09563.1"
                     /translation="MTNSSANESRVPVLGINSLGRIGKLTLWHHISRKYFKEIIVNQG
                     RDIGMGMETIARLIEADATYGLLHRFLYGIKAQPCIQITDDKNGKMLIDGIPVTILRE
                     QRNPLNIPWRQYGADIVVDCSGSFKDPTVPVDDKKGSIRGHLNGGAKVVIHSAPFKIK
                     NKALATPEDTTTLIYGINHTAFNPKKHLLVSAASCTTTGLAHMVKPLLDNEETSTILT
                     ASMSTIHAVTNTQSVLDKLPKAGEKDIRKTRSILNNIILTSTGAAKALAEVIPEVKNI
                     GFMGDSIRVPTNTLSLIVLNATFQARNNDKAAAAGLDTKKLNEIYAKAAGNNPLVRFT
                     MQQNVSTDLIGEDAAVIIEGQFNHTRTAFIPVDLSHIPNLPADLVKSLGEKLLQVPVV
                     HAKIFGWYDNEYGSYTNRMGDLTVYIHKNLQ"
     gene            complement(31149..31475)
                     /gene="hgcB"
                     /locus_tag="PLQ77_02410"
     CDS             complement(31149..31475)
                     /gene="hgcB"
                     /locus_tag="PLQ77_02410"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HPL09564.1"
                     /translation="MGNLTYLKGVATLQMDEKKCTGCGMCIEVCPHHVFEMNVKNAMI
                     INFDACMECGACRQNCPSGAISVQSGVGCAAAVIGSLLGRNGGECCCGPRTDETGAQK
                     SGGCCS"
     gene            complement(31567..32715)
                     /gene="hgcA"
                     /locus_tag="PLQ77_02415"
     CDS             complement(31567..32715)
                     /gene="hgcA"
                     /locus_tag="PLQ77_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264175424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPL09565.1"
                     /translation="MDIKNGFEFPVLQEPAGCGCGGPAVPAADDPIARLDQSFVSGSF
                     STPAGDLPKVSSLLTRQDRWGTIKARLGAGRMNYAIDPGLYALGNPDDQSPVLVTANY
                     KMSFDCLREALPNRNAWILVLDTKGINVWCAAGKGTFGTQELLRRIETSGLKKLVSHR
                     KLILPQLGAPGVSAHRVRQSTGFQVYYGPVRASDLPAYLDGKMKKTDQMREITFSMKE
                     RTVLIPIELLSTAKSFLLVSSVLLLLSGLLGPNGFLINIQEIGGFAVAALFCAVLGGA
                     VLNPLLLPCIPGRAFSVKGFIIGLVIALGLIFLRGMDLQSGAGKLEALSWLLIIPAVS
                     AYLAMNFTGCSTYTSLSGVKKEMRLALPLEISAGVFGLAGWIASLVVL"
     gene            complement(32732..33193)
                     /locus_tag="PLQ77_02420"
     CDS             complement(32732..33193)
                     /locus_tag="PLQ77_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPL09566.1"
                     /translation="MKKKEGHPAKLIDVFVGLAQATRCFRQDTAFCGGVTFHQFIILN
                     AAAKNKYLRISDLHSLLAVEKSTTTRLLNPLLEKGLLTREKSASDSRIFGLTLTKSGE
                     ETHRQVQLCLEEFFNKVAANLPAGKEENILQAVQIWINAIKNAAGVCNGCQ"
     gene            33484..33933
                     /locus_tag="PLQ77_02425"
     CDS             33484..33933
                     /locus_tag="PLQ77_02425"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPL09567.1"
                     /translation="MKTYSDAAEEKFIEGYNCAQSVLFSFCEELGLDADTALKISCGF
                     GGGMGRKGEVCGAVTGGVMVLGLKYGRGIKEQREQTYASYVKIRELFDRFSARHSTCL
                     CRELLGGCNLATPEGQAYFKENDCLRLVCRPCVRDAAELVCDLLAEG"
     gene            34082..34864
                     /locus_tag="PLQ77_02430"
     CDS             34082..34864
                     /locus_tag="PLQ77_02430"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPL09568.1"
                     /translation="MTINIKAEEHIMLIEIDRPKKYNAMTREMYSQMAQAYYQLDQDD
                     HYRVGVVYANGPHFTSGLELTDWSDVFTGGAGFPLAEKNEIDPFYMMSDARCRKPIIF
                     AVQGYCFTWGVETMLNADIRVAASDTRFGMLEVRRGFFPCGGATLRLPKEMGRANAMR
                     YLLTGDEWTAEEAYRTGLVQHVTAPGKQLEKALELARRVATAAPLGVRYILKNSRTAE
                     LQGEKAARQDIFQDVAGVMKSEDMREGLQSFIERRAAVFKGK"
     gene            34894..36720
                     /locus_tag="PLQ77_02435"
     CDS             34894..36720
                     /locus_tag="PLQ77_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL09569.1"
                     /translation="MAHSNWLYQTRDIMFQIKEWLGVEKLLGLDAYKDYYGMDDIDSF
                     LDVNFKVCRDVMCPANKDADEPGAKFAGGNEHAVTTPDSFKNVYKTIMDAELGPQFGY
                     RGEGKIPLCWYAPILEMQSAASPSIVMFWCLTQGAITVLHEFATQKQQDLFIPKLRTG
                     EWGGTMGLTEPGAGSEVGAVQSKAFPTDTPGKYKLTGTKCFISSGDHDLAENIIHLML
                     AKTPGAKEGTAGISLFIVPKFWVNEDGSLGAWNDVTTMGIEHKLGIHGSTTATLSMGE
                     NDNCYGWMVGEAEVKDGRGIGMKQMFAYMNEERLNTGLFSLGCIGSAYYAALDYAKLR
                     VQSKHSTNPKGPSVRIIEHEDVRRMLLSQKSVLEACRALIYKSYLYRDLAVDAATPEE
                     REYYDDMFAINNPMCKAYTSDMARLTTGDAIQCHGGYGFTEEYAPAELYRDVVIYGIW
                     EGTNFIQAQDYTGRKFTMKDGAPFKKWVAEIDDFVANKKTEAFAAEFAMMAEAMTAFK
                     EIVNMNDAWTKGDRQMKQLFATRTLHAGARVYCGMLLLDQALLAAAKMKEKGEIDADF
                     YKGKIATARFYVMNHVPDIFGYLKTMKYGDRSAIDIPEESFM"
BASE COUNT         7776 a        10171 c        10530 g         8280 t
ORIGIN      
        1 cgccgttggc ggtgtccaga acgattttca tgccttccat ggaaagattg cggggaaagg
       61 tgtttttggc aaagacgatg tagcgtccgt tcacgccgtc caggcggtat gcctttccga
      121 tgtcctgacc cctggacacc aggtcggaaa gtttgttgtt gaggatcagg tcttcgatga
      181 attcctcctg ctcgtcggaa agcttgtagc cgtcgccgcc gaaaatcttc aggccgttgt
      241 cctgaaaggg attgtgggac gcggaaatca cgacacccgc gtcggcgcgc atgctctggg
      301 tgacgaaggc gatcgccggc gtgggcatca cgccgaccag gtaggaatag ccccccatgg
      361 acgtgatgcc ggcttccagc gagctctcca gcatgtagcc ggaaagccgg gtgtctttcc
      421 cgataatgat gacggcccgg tgtccgttct ttttaaacaa atgcgccatg gcccgcccga
      481 cgccaaaggc gatttcaggg gtcatgggat attgattcgc ttctcctcga attccgtcag
      541 tgccgaataa cttgcccata aatggtctcc ttgatgttca ttgtttcagc tatttagcaa
      601 aaaccgggtc tgtgcccgtt tcagttcctg aataatccca tcgacggcat ggcccgcgca
      661 gcctgattgc agagcgcccc gacgatgccg tttaaccatg ccgtcccttt tgcagaaacc
      721 gccccgggca gtttccggac tgctccgacg tagtcggttc tgtcatttcc cgcgcttttt
      781 tcgaagtctt gcagaaaggc ttcgagaagc ggatccgttt tcgtgaagcc gtccggtatt
      841 tttccggtga agaggtcttc gcagaaaccg gtttgttcaa gaaactcttt tctggcttcc
      901 tcttccggcc cgggattggc ggcggctttt tgggccatcg cctttagcct cttcagccgc
      961 tcttttttgg ccgaggccag cgtctccaga acgcccgcgt aaatcagcgg gccgaacggc
     1021 cgggcatcca aaaacggcct gcggacgtgc gcgtaccagg attccagagc ggacaggttg
     1081 gccagataca ggatattgtt ttccacgatt ctccgcaggt tcgggtaagc ggcgcgacga
     1141 tagtttttcg cgccgtccgc gggcgttgtc tcaaaaatca acaggccgtc atcgggataa
     1201 tcatggcgca ggaccgagcc cgcggccgcg acgttgccga atcccatgcg caccggaccg
     1261 atgatgccgc cctggccgcc cagaaaaatg gggggctggt tgagcattac gccccgggga
     1321 acatccccca ggagggaagg ggttgttttg tccgcgtccg gcgtgaaatt gaagtgaatg
     1381 taggaactgc ccacttcgga gtggttgctg cggctggtcc cgccggccat caggcagtcg
     1441 cagaagttga tcaggctgcc cagcgtgaca aagggaaaga gaatggtctg tttcaggccg
     1501 accgagtggg cgccgcccgc ttcttcctcc aggaggcatc cttcccggac gtgcgcgccc
     1561 attccgaggt ttgccttctc cagaaaaacc gatttgctga agaagcctcc cttcagctcg
     1621 acgcgctctc cgatctgaca gtcctcgatg gtcgccggcg cttcataccc cagcttgcag
     1681 ccggacgaga tgacggtttt cccgccgtaa atccggcatc cgggatagat cgctgtgccc
     1741 tttcccgaaa tctgatcgat cttgacgtcg tcgccgatgt ccagcgtcgc cggattgggg
     1801 atgtttaccc ctttgtccag cagctgctgt attttttcat gggatctcgg cttaaaatcc
     1861 atacaggccc ccattggagt gatgagtgga aagtgaaaag tgaaaagtga agccgatcac
     1921 tcatcgccaa tcatcttgtc ttttttacta tttaccatcc acttttttta ctatgagctg
     1981 tgagcttttt cccctatcgc ccatagccca tagcctatcg cctttttatt atactacccc
     2041 gtaagccagc atggctttgg ccactttggt gaagccggca atgttggcgc ccgttacata
     2101 gtcgcccttt ttaccgtaag cctgcgccgt gtcaaagcaa tttttatgga tgcttttcat
     2161 gatgatcagc agacggttgt ccacttcttc gcgcgtccag gaaagccgca ggctgttctg
     2221 gctcatttcc agaccggacg tggccacgcc gcccgcgttg gccgccttgc cggggccgta
     2281 cagaatcttg ttctcctggt atattttgat ggcctccggc gtcgaaggca tgttcgcgcc
     2341 ttcggccacg cagtagcatc cgtttttcac cagggcggct gcgttctttc cgttgatttc
     2401 attctgggtg gcgcagggca gggcgatatc cactttgatg ccctgttcgc ggatgacgtc
     2461 ccagacgttt ttcccttcaa agcaggtggt cgattgtttg tacttgtccg cgtattcact
     2521 gatgcggccg cgtttgacgt tcttcagatc caacacatag ctgcatttgt ccatgtcaat
     2581 gccggcgtca tcaataatgc aggaggagga atcgcaaagg gaaatgacct tgccgcccag
     2641 ctgattgact ttttccacgg caaactgtgc tacgtttccg gcgccgctga tggccacggt
     2701 cttgcctttg aaatccagcc cgcgcgttgc cagcatttcc gcggcgaagt aggcggtgcc
     2761 gtagcccgtt gcttccggac ggatcaggct tccgccgtag tccagaccct tgccggtgag
     2821 cactcccgtg tgttcgttgc ggattttttt atagtagccg tacatgtagc cgatttcccg
     2881 cgcgcccacg ccgatgtcgc cggccggaac gtccacatcc gcgccgatat ggcggtacag
     2941 ctcgcgcatg aaggactggc agaatttcat gacttccgcg tcggatttgc ccttcgggtc
     3001 gaagtccgag ccgcccttgg cgccgcccat gggcagcgtg gtgagcgcat ttttgaagat
     3061 ctgctcaaat cccaggaatt tgataatgcc cagatttacg gacggatgga agcggatgcc
     3121 gcccttgaac gggccgatgg cgttgttgaa ctggacgcgg aagccgcggt tgacccgcac
     3181 cttcccctga tcgtccatcc agggcacacg aaagatcacg gccctctccg gctcgacaat
     3241 ccgttcgtag atgctggcct ttacgaattc agggtgcttt tttacggtag gctccagcgt
     3301 ttccatgact tccgcgaccg cctgatgaaa ttcgggctgg tccggatcgg tctgtttgac
     3361 tttctcgatg acttcttgaa tgactgacat acggttaatc cccctgtaaa tatgattttt
     3421 tggttaaatt gtctcaatat cgcgcatttt ctcaaattcc atgacggtgg acatggcttt
     3481 gttgtccacc gtcagtcctt cctcgcaaac cgccgccacc ggattcaggc cgccgatcag
     3541 aatcatcccg attttattca tatccacatg ggtctgacaa accgggtgtc caatgtggcc
     3601 gatggaaaga acgccgtgca ggccggcccc ggcgaaatcc tccatcaccc gctccaccag
     3661 agggaggctg agcgcgggga tttcccggaa gttggccaga atttttcctt cgccggtcct
     3721 ggcgacgctt cggacggacg tcattttgcc gcggatgaag atttccgaag ggtccagcga
     3781 ggagccggcg tagtgaatca gctccacgaa acgaaggatt ttcccgccgc gcatctggag
     3841 aatgccgccg aatttggagt cgatcggaat gccgtgcttc aacagaacgc cgttgatgac
     3901 gatgctgcag acggtggcga agccgacgct gccttccggc accgcgatgt cccccaggct
     3961 ttttccggcc tgcgccacgg ccacgcgcga gctgacggcc agtttttgct gaaacacggg
     4021 cttcatgatg gagagggccc gggaaaaatg ctctttcgga aagaaggaga cgttgaccgg
     4081 caccaggccg cgcttctttt tgacgtcaaa ggtggttcga aacgagagaa ggtcgatgcg
     4141 cgaaatggaa aggccgatct tatcctgcac gcgggcgtcg gaaatttcgg caaatccctg
     4201 ttcggtgatt acgcggccgt cgcggcggcc cacgagtttg gtgagccccc gttcatccat
     4261 gatcttcagg tggtagcgga ccgttctctc gctgagctgc acgtgataat ccgacattcg
     4321 gcgggcaatg acgcgcgcgc ccatcggatc tgggctttcg tggaggatct tcagaatcag
     4381 aagaaccttc cgttcaatgt cttctggttt aaaaagctct ttcattcgac ttccaatcgg
     4441 caatcggcaa tcgtttgccg tatccctact tgcaaaccgt ttgtttgtca aataaaattt
     4501 tcggccgctt cgaaagagcg ctccggaagg caaaaagagc ggggaagggc ctcacgtgaa
     4561 ggccgtgttt ttcacggggg aagggttgca gcgggcctga atccgcctgg cgatttcctc
     4621 gacggattcc ccaaacaggc attccagcgg cagcgcctcc atgtaccggt cgttccacgc
     4681 caggttgtat tcgttgatta aattgcggat gtcgtcatac cggtgtccca ggacctcgat
     4741 tttttccggc ccgctcaagc gctcattgaa ggcgatatgg atcagcagca ggtttccgat
     4801 ccggccgtcc gtttcctgca gggggatgac catgatggaa gcggaatcga tttttccccg
     4861 gcccagataa gcatggcctg tgctgacgat ggttttcttg acccccatca gagttgtggg
     4921 ctgctccgcg cgggaggaca tgcgcagggc tgcgccgcct ttggcgcata ccgcaatggt
     4981 cgaagccggt ccgggatttc ccttttcgtc gaggccgcgc acacggtaga gcgtgtagcc
     5041 ttccaccgcc cccacggacg gctggaggcg gtgaatcgtc ctgatgttgc gccaggtcag
     5101 attttttacg gagaagttca gcgctccgag caggtcgaaa acaacgccct ggggctcttt
     5161 ttcctttcgg ctggttccca ccgtcacggt tttggcctga tggcggatgg cgtcgatcgg
     5221 acgggtcagt tcatcaatgg cggctcccag ggtttcatcc agcaggtcga acggctggac
     5281 caggtcttcg tgtccgggga aatcctggcg gatgtcctga agcggcagtt tcccggatgc
     5341 gtacttcagc agcagcgtga gatcggcgat ggttttgccg cccagcgcgc tcaacgcgcc
     5401 gccctgaagc cgggattgaa accggctgca gaaatcgttg atgatccggc ggaaggaagg
     5461 atcggccatc agatcaaaaa cgtccaaaac ggataacttc gtttttttct tttgcagccg
     5521 gccgagcgcc aggcggccgc ggaacgccct gaagagctgc gcttccccgt cgatggcgca
     5581 ggccgcgtaa tagccccaca ggtggcccgc catggtattg agaatcacgg aaaggggggc
     5641 gggtgtcttt ggaacggcaa tgaccgcgtc ggccgcggga tgaaagcgca aatccccttc
     5701 atcggcgaaa acgacggtcg ccgctttgtg cgcccgaaag atggcgatgt ccttcgcgat
     5761 gtcgtccagt acgacatcgg gattgccggc ggcgcagacc agaatcagcg gctcggcgga
     5821 caggtcgatg tgctttttat tttccacgat gtccgaagaa atggttttgt agcacatttc
     5881 ggaaagcttg atgcggattt cgtcggccgc cgccttgttg gggccgctgc ccacgacggc
     5941 ccagtagcgc ttgcccgcgc cctgcgttac acaggcggcg atttcgtcgc gccggtcgaa
     6001 caagcgctgc atggcttgcg gaatgttttc aagatgccgg atccggcgga caatggcttc
     6061 gtcgctcatc gtccccagca gtttggcgaa atacagggcc agcaactgtc cggcaacgag
     6121 ctgggcgtag aaggccttgg tggaggcgac ggacatttca atgtcccgtc cgtcgctggt
     6181 gtaaaaaacg ccgtgcgacg aggcggtgat atccgactgg cggcggttga cgatggagag
     6241 aatctgcgcg ccgcgcgccc tggccatgga cacggcccgg ttggtgtccg tggtggttcc
     6301 ggactgcgtg atggggatga ccagcgtatc cgacagatcg tcccgaaggc cgaagccgct
     6361 catttcggac gcgacgcgcg actggatatt gagacggctc atgccgaggc agtgcgccag
     6421 cgcatccgcc accgcctgtc ccgccaccgc cgccgtgccg tggccgatga cgaagatgtt
     6481 ccgcgtcaag ccgctctgca ggccgtttcg aatcgaggcg ggaaccatat cctcgcccag
     6541 attgaaaaga atcccggacg gagcggaggc ggcgccggtg atccggtatt tcccccgcag
     6601 cgttccctgg acggaacgga cggattctga aatttctttg agaaagaaat gggggaaaca
     6661 gccgcggtca atgtcccggg tcgtgatttc ggcggtttgc agattccgct cttccagatg
     6721 aatcggggct ccgtcgtagc agcaggcggc aatgcccttt gcgcccgtgc ctcggttttg
     6781 atccagaatg aacagttgtc cgcccgcctc ctcgccgttc atccggatga agcggggtgc
     6841 aatctccacc agtccataga gctccgagga aatcatgtac gcgtcttctc cgacgccgac
     6901 gtaaatcgac tggccgttcc cctgaagcgc cagaaatatt ttgcccggct ccagattgct
     6961 ttgcatggca atggcgtgcg aaccttcgaa atcgtttacg gccagcctga aggcgtctgt
     7021 caggttttgc ccttttctca gatatttttc gatttgcagg ggaatgattt tggtgtccgt
     7081 cgagacgtcc ggcgcgattt gctccccgtc ttgctccagg tccctgcgaa gcgccggata
     7141 attgtcgatg tcgccgttga gcaccacgct gatgtgcgct tcccgccccg gatacgcggg
     7201 gaaatcggac aattccggac gaagggttgc gttgttgacc ggatgacaat tttcttcggt
     7261 gatggagccc acggaggccc accgggtgtg ggcgatggcg gattcgtccg ccgacgcccg
     7321 tgcggcaaac cattgaagaa tggcgtcgcg ggagatccat tgccgcaggt cggcgacgtt
     7381 gcggcccagt tcgccgacca cggaaaaggt tttgtaggta aaggcaatat gaattccgcc
     7441 gccgggattt tgagcgccgc cggaaataaa aatggaccgg tttatgagat cgcctttttg
     7501 cgtccggcgc cggtaatcct ccccccatcc gttttcttcg atccgccggt ccagttcctc
     7561 aaggaccttt gcgtctttga ggaaaaaagc caactggatg cccgccgagt cccgtccccg
     7621 gacctccaga cgatccagcg cgtttaacag cagattcagc ttccggtatt ttcgaaaggc
     7681 ggccggttga agcgaagaat atttttccgc tccggaaagc gagagaactt tttgcaggtt
     7741 ggccaggatg tcgctctcca gcatccagca aatgtccttg agcgagagca gccggcggtt
     7801 gacggtttca aggccggcgg agctcagccc tgccgcccgg tcctccagaa ttttctcttc
     7861 cgcggccaga aaggctttca tcccgccggc gacgcgggtc aaatccgccg tccggtcttt
     7921 ctgaaaaaaa agacattcct ggacatcttc ccgcttcagc cggttgacgc aatccgcgag
     7981 agactggatc gtttccgggc cgttcaaata accgcccggg tcggtttttc cgccaaagac
     8041 ctgttccagc ccgttcttat ttgctttttc ccaaagcttc cgaagggttt gatccgccgg
     8101 atcgccggga ttctccggcg ccggttgaaa ggacagcagg cctgcgaatc cgcaattcaa
     8161 tctgccgggg aggatgggag aaaaaatgat tgcaggtccg ccaactgatt ccggccgaat
     8221 gccgaaaaaa acccggcaat gtgttacaaa aaagagccat ctttttatca gaagagacaa
     8281 ttttgtatag aacatcattt cctcttgaac tgttgcgggg cggttgtaaa acgaaaccgg
     8341 ccgcggcggt tttgtcgaca agaacatcag tccccatgcc gcggctgagt cattcgcgcc
     8401 gcccctgggg attgcgatga tggatttggc gccgtccaat tcttccgctc cctgcttttc
     8461 agggggcttt gccggatgca ttacgcaaaa accattcctg aatttctcag gccgattccg
     8521 cctggtgcga cgccgaagat gaaaaagccg aggcctttct cttttggatt gggaaagcgg
     8581 ataagggatg cgaccctgaa gatcctgtgc gggagagttt cagggcggag cattccccgg
     8641 ggcggagagg ctccgatgcg gggtaaatca tttcagggat ggatgggaag agaatctcta
     8701 ttctttttcg ccgtcgaggc ccagcttggc cattttatcg gtaaaggtat tgcggtggat
     8761 gccgagaaat gccgccgccg agcttttgac gttgccggag cgccttaaag cgattttgaa
     8821 aaggcccgtt tccaccatcg ccatgacctc cgcgtgaagt ttgcttctgc cgttgtcgga
     8881 gcccgacagg accgtcgcca gatcatccag ctttttttcc agaatttctt cgacggcctt
     8941 gttgagggcc tcctgaacgt ctattcccgc ggccgggtta atctgctttt tcgctttcat
     9001 gttcatgcag ctcctgcgtt tcaaaacatt gtttcattcc aaccgttccc ttcagaattc
     9061 cggacgatcc gcgtgaaaac atcaggccgc gagccatcgg gtcagcacca cggcaaccaa
     9121 aagaaccagg gcgacccagg tcagcacgat gagcgatgtc cgttctttgg gggcttcgat
     9181 gtaatttttg atttccgttt ccactctttc atcttccagc ttctccgcga gctgcccgag
     9241 caggcgcgtg tggtcctgca ggaggttgga gaagagataa aatcccttgg tcgtcgtgag
     9301 caggaaatag gccttgctgc gcaggatgac cgtgcccagg tgcgtgattt ccgcccaggt
     9361 gaaatgcctg gtccggaaaa attttcgaat ccgcaggccg ttttcggaaa tgccgaattc
     9421 ccgctccgag gattcaacgg ccacgaccag cgcggcggca aacaaagcgc ccgtcaggat
     9481 tttttcccag cattcgccgc gcgccagccc cgtcagaaac agaacgaaca gcaggaccgt
     9541 cagcgtgacg aaaggataac ggaaagcttt cctgattttg taaacagcgt tcacgcgttg
     9601 tctccgaatt attttgcggc ttttcggacg aaggcgccgc tcttgccgcc ggatttttcc
     9661 agaagcagaa tctcgccgat gaccatttcc cggtccacgg ctttgcacat gtcataaatg
     9721 gtgagcgccg cgacgctcac cgcggtcagc gcttccattt ccaccccggt ttgcccgacc
     9781 gtccgcacct gcgattcaat gaccacccgt gatttttttc ggtcgaggac aaaggtgatg
     9841 tccatgccgg tcagccccag gggatggcac agcggaatga tgccgccggt atttttggct
     9901 gccagaatgc ctgcgatttt agcggcggtc agcacgttgc ccttggcgat gcgtccctcg
     9961 gccagcagct tcatcgtggc cggcttcatg gttacctggc cgcgggcttt cgcctgccgg
    10021 tgcgttgcgg tttttgccgt cacatccacc atccgaacct gaccttcatc gttgatatga
    10081 gataattttt tcatggtgac ccggaacttt tcggtgtttg acggttattg ggatttcttc
    10141 aatttcagcg aaccctagca tagcgtcttt accgagtcaa gaatggcgaa gaagaatttt
    10201 tgcaaattaa aatcttgata taaatgaatg gattcgctag tatttgcgcc gcgaattctt
    10261 ccccggagaa taaaagtgtt catcaaagtg cagagcatga gcgtgatcgg catggaatcc
    10321 tatcctgtga cggtggaagt ggatgtctcc cgggggttgc cgcattttgc caccgtcggc
    10381 ctgccggacg cctcggtcaa ggaaagccgc gaccggatca aggccgccat caagaattcc
    10441 ggctacgctt ttccgcgcga tcacgtgacg gtgaacctgg cgcctgccga catccgcaaa
    10501 gagggaaccg gctttgatct gccgatcgcg gtgggcattc tggccgcgga ggcgctgatt
    10561 gaagaagcgg ccctggacgg ttgtttcttt atcggggagc tgtcgctgga cggcagcatc
    10621 aagggagtgc ggggcgtact gcctgccgcc ttcaaggccc gggaatctgg aatccgttcc
    10681 gtttttgttc ccgaagaaaa cgccgcggaa gcggcgatgg tggaaggcat tcaggtttac
    10741 ggcgtccgga cgcttcccga cgtcgtcgag tttctggctg accggaaaat catcgaaccg
    10801 ctcagcgtga atgccgaccg gctttttcaa gtccaccgtc attacgaaat cgatttttcc
    10861 gaaatcaagg gccagaagca ggcgctccgc gcgctggaga tcgccgcggc aggctctcac
    10921 aacgttctga tgatcggtcc gccgggctcc ggcaaaagca tgctggcccg aagactgccc
    10981 acgatcctcc cggagctttc ctttgaagaa gcgatcgagg taaccaagat tttttccgtg
    11041 gcgggacttt tgaatgcggg ggaaacgctg gtggcggctc gtccctttcg agcgccgcat
    11101 cacaccattt cagacgcggg cctggtcggc ggcggtcaga tgccgcatcc cggggaaata
    11161 tcgctgtccc atctgggcgt attgtttttg gatgaacttc cggagttcaa aagaaacgcg
    11221 ctggaggcgc tccgtcagcc gcttgaggaa ggcgcgatca cgattacccg ttcggccgta
    11281 acggcggcct ttccggcgag attcatgctg gtggccgcca tgaacccctg cccgtgcgga
    11341 tattttggag accgggtccg ccgctgccgc tgctcgcccc agcagatccg gcagtatcag
    11401 gcaaaaatct ccggccccct gctggaccgc atcgaccttc atgtcgaggt gccgtccatc
    11461 cagtacaggg cgctctcggg aaaacaggaa aatgaatctt ccgcccatgt cagagagcgc
    11521 gtggtccggg ctcgccgccg ccagaaggag cgcttcggcg gcgcgggcat tccgaccaac
    11581 gcctccatga cggaaaagca gatccgttct ttctgcccca tcgatgaaga gtcgcatcag
    11641 ctcatggaaa tggccgtcga taagctggga ctttccgccc gcgccctgga ccgcatcctg
    11701 aaagtgtcgc gcaccattgc cgatctggag ggcgccgcga agatcgaagc cgcgcacgtc
    11761 gccgaggcga ttcagtatcg aagccttgat cggaacatcg tttagtgcta taaaaaaacg
    11821 aagccggccc cgaatggccg gaatcttttt gccaagagga tcctatgaaa attaaaatta
    11881 tcaaagcgct tcccaagtac ctgaagccca agccggacga aaaaaaactg ggattcggtc
    11941 agcatttcac cgaccacatg tttaccatga agtacaggga aggagacgga tggcaggatc
    12001 cggtcatcga accctaccat tacctgcagt tggatccaac cgccatgtgc cttcattacg
    12061 gccaggaaat ctttgaaggt ctgaaggcgt accggggggc aaacaacgaa atttatcttt
    12121 tccgtccgat ggagaatatc cgcagaatga atgtgtccgc cgaaaggctg tgcatgccgc
    12181 ccatggacga aacggtcgtg ctgaacgcgc ttcggcagtt cgttgtgctg gaaaaggaat
    12241 ggattccccg cgagaagggg tcgtccctgt atatccggcc gacgatgatt gccacggagc
    12301 cggctctcgg cgtgcatccg gcgagcgaat atctgttctt catcattgcc gggcctgtgg
    12361 gcgcctacta cgcgcacggc ttcgggccga ccaaaattta tgtgtccgac gactgcgtgc
    12421 gggccgcgcc gggcggcact gggcatgtca aggcggcggg gaattacgcc gcaagcctct
    12481 acgcgagccg cattgccagc acgatgggct atacgcaggt cctgtggctg gacgccaggg
    12541 aaaaccggta cgtggaggaa gtgggcacca gcaatatctt cttcctgatc gaagaccggt
    12601 tgattacgcc tccgctggga ggaaccattc ttccgggcgt gacgcgcgat tccgtcatca
    12661 aactggcggc gcacctgggc gtgaaggtgg aggaaagccg gctgaccatc acggaggtga
    12721 tctccgcgat tgaaaatgga acgctgcgcg atgcgtttgc cacgggaacg gctgccgtga
    12781 tttcaccggt cggacagatt ttttatcaca accgggaata tctcattaac gatggaaaga
    12841 ccggggagct ggccgaaaag ctatacaatg aaatactgca aattcagtac ggaacgagag
    12901 aagatccctt tggctggaga atgcagattg actgaagatg gcgtaaaaaa tcaagtttta
    12961 aaccatttag gcgcttttgg ccctgaagga aaaccggatt ccacaaagct gtggaaaggc
    13021 ctgttgacaa ccgtgtggat aatccggtca agtccgcatt gcttcggatt ttggaccgga
    13081 ttgcttaaga tttaagcaat aatttatatg agtaatattc aatagttacc catatacctt
    13141 ttaatcataa cgacttatgg ctttctcggg cccaagatat aaactgcatt gaaaaaaatg
    13201 tgccattacc gagagggttt tctctgttgc gggaaataaa aaaacatgaa ggcaaagctc
    13261 aataaaattg aattgttttt cccggccggc gtcctggcgt tttgcctggc tctcttttgg
    13321 gcggttgttt ttcccggtgc ttccacagcg gccccgcccc ttgaggaagt ggttgaccgg
    13381 cttcagcgga tttatgaaaa aacgcaggat tttcaagccg gttttatcca ggaaaccacg
    13441 gtgaagtcca ttcggaaaac ggacacggaa tcgggaacgg tttatttcaa gaatcccggt
    13501 cagatgctct gggattacaa aaagccgaag gcgaaaaaac tgatcatcaa cgctcagaag
    13561 gcatggctct acctgcccca ggataaaacc gtttacgtgc aggattcggg aaagattatg
    13621 aaatccgaag ccttaatcaa atttctgtcc ggtctgggga agctgaataa tgacttcacg
    13681 attcaatacg cctctcccgc ggaggatccg gacggcaact gcctgctgct cctttatccc
    13741 cgggaaaaag gagcgtccta ctcctttctg agaatgacgg tggcaaaaaa caatttttac
    13801 attctccagg tcggttttga cgacgtgatg ggcaactcca ctctcctgag attctccaac
    13861 atcaaaatga acagcgggct ttcctcgaaa ctattccagt tccagcctcc gcccggcgtc
    13921 agcattttta aaatgtaggg gacgctgtta acttattaac tttctcgttg acaacgcgga
    13981 aaattcggaa tattgaaaac gccatgccaa gaggagcgcg tatagatgca gccgacgccg
    14041 tccaccacat catggcacgc ggcatcgaga gcggcaagat attcgattct gatgccgatc
    14101 gggataattt cctttatcgg ttaggagata tttttccgga taccgcaacg acttgttttg
    14161 cttggtcatt gatgcccaat cattttcatc tgctttgccg cacgggcaat attccgattt
    14221 cgaccgtgat gaggcgactg ttgaccggat acgccacttg gtttaaccgg agccgaaagc
    14281 ggcacggcca cctttttcag aatcgattca agtccatttt atgccaggag gatgcctatc
    14341 ttcttgaact ggtacgctac attcacctca acccgctgcg ggccggactg gttcagaatt
    14401 tgaataagct ggacagctat ccgtattccg gccatagcgc cctgatggga agagagcatc
    14461 gttcctggca ggaaacgcaa ggaattctga tgctttttgg caacacgcag gatgcagctc
    14521 gtcagtccta tcgacagttt gtcgggcagg gaattgaaca aggcagacgg catgatttga
    14581 caggcggggg cttgatccgc agcgctggcg gctgggaggg tctcaagcgg aaatgcgagg
    14641 aagggactta ccaacgaagc gatgaaagga tactgggtga cggtgatttc gtcagtcagg
    14701 ttttgtcaaa gacggaagag aagatcaaga aaagttatgc gcttcgaaac agcggcgtgg
    14761 atctggatca agtggccttg cgcgtttcgg cattgacggg ggtcaaaccc ggtgacgtcc
    14821 gtacggcggg taagcaacca catatcgtca acgcccgaag cctgttttgc ttttgggcgg
    14881 ttcgtgatct tggagagacg atggccggtt tggcccgtcg tctcggctta tccattccgg
    14941 ccatcagcaa atcagttgtt cgaggccggc agattgccga atccaacggg tttgaactga
    15001 tgcaaaacaa ccataattaa acgtttgaat ttattaaata cgtaataaat acaaatatat
    15061 tgaatagaaa gttaataagt taacagcgtc ccattaattg aagggatgct cccgcttgaa
    15121 atccggcgtc agggctctgt catcgacttc ctgaatgaat agattttcaa acccgagcct
    15181 aagggcaaat tccacaattt catgatattc cgaggccgta accctgcggc ccaaaagcgg
    15241 atgcgagcgc acttttggaa cgggcgtgta ttgagacatg aggctgatcg gaatgcgcgc
    15301 ggagacctcg ccgcgcaaaa gcctcagcgc ctcctttgaa ttttccaggc gccccggcag
    15361 gaccagatgg cggataatga cgccgcggga agcgatgccg ttttccacgt ccagatcatc
    15421 ccccacctgc cggaccatct cctgaaggga ggcgacggca aacttcgaat agtccggcgc
    15481 atcggagaac attcgggcgt cttcttccag accgtatttg aaatccggca gatagatatc
    15541 gaccatgccg tccagcatcc ggatcacgtc cggattttca tagccgccgc agttatagac
    15601 cagcgggacg gtgagcccct gggcccgcgc cagacagagg gcttccatca ccagaggcgt
    15661 ctggggcgtg ggggttaccg gctcgacatt gtggcagccg cttttctgga gatcgatcat
    15721 gatccgggcc agttcgatga cggtcaaacg gcggccttgc gccctgtgcg aaatttgata
    15781 gttctggcaa tagacacatc ccagattgca ggaagaaagg aaaatggtgc ctgcgccgcc
    15841 tgttcccgaa agcggcggct cctctccgtg atggggcaga gcgcagtcca tgacgatgtc
    15901 ggaaggcagg cggcagtagc ccggttcgcc tttcgtccgg tcaacatggc aggcgcgcgg
    15961 gcaaagggcg caggatcgat acatgtcaag gagttcggga atgatcataa acacaagaat
    16021 ggcgcaggca gcagaccccg gattacagcc gacggcctgc tgcctgaaac agcaaaagaa
    16081 aaaacgtttg cggaaacagt ccggccgttc gcgttgccgg gagagaggcc gcgacggctc
    16141 cttgccttgt tttgattact cgccggtgaa gttcggttcg cgcttctgca ggaaagcggt
    16201 gatgccctcc acggcgtcct tgctggcggc cagtttattg gaccactcga tgtccagctt
    16261 caggccttca ttaaagccct tttccattcc gacgctgatg ccttccagaa cggcggccac
    16321 ggccagcggc gggcgtttgg tcagcgcctg ggcaaatgcc gtcgcctcct tcatcaaatc
    16381 ggcggccgga acgactttat cgaccagtcc cacggccagc gcttcggtgg gcggcaggcg
    16441 tttgctgaag agaatcaact ccagcgcctt ggattttccc agaagcctgg gcagccgctg
    16501 aatgccgccc cagccgggcg tgatgcccag attgacttcc ggcagtccga tgagcgcctt
    16561 ggggttgtcc gtcatgatgc ggaaatggca ggcgatcgcc gtttcgcaac cgccgcccag
    16621 ggcgtgcccg ttgattgccg cgatgtaggg tttggcggag cgcgagatgg cgttcatgat
    16681 gtcgttgccg ttgggtccgc gggtgatgtt ggccacgtcg gagacatcca ttccggcgga
    16741 gaagcctttt tcacccgcgc cggtgacgat gacagcccgc gtttccctgc tcttttcaat
    16801 gttcgtgacc gcgtcgaaaa attcctcgcg aatgcccaga ttcacggagt tcatcggcgg
    16861 gcgattcaaa acaatcgtcg tcacgaaatt ttcggttgtc acaataatgt tcttgtaatc
    16921 catctctttt ctcctttcta atggttttat ggttgatttt gagtcttttg atggccattc
    16981 gccctgtcgc tgacccgtac accttcagcc gatgacctat agcccatcgc ctatggcctg
    17041 cttttctgat gcaccagccg ctggcccgaa tgcggcactt caaaaaaggg aacgttcgcg
    17101 ccgccaaggc ttccgataaa agccgtgcgg cggtccttcc cgccgaagca gatattggcc
    17161 ggacggttca gcacctcgcc ttgcggatcg tccgcgcaaa tctccaggcc gccccgcgga
    17221 tcaatgcaac cgacggcatt ggcgacagga aaggcaatcc agaggttgcc ggcttcgtca
    17281 aacgcgatgc cgtcgggaaa gccgagcttg cccaggaatt caggaccgta aacctctgcc
    17341 ggtccgaccg tgtcgtcggc atgaatcctg tagcgcagaa tgcggcgttt catggtttcc
    17401 gccacataaa catattgttc gtccgcatcc accgcgacgc cgttggcaaa acaaatgcct
    17461 tcggcgacta ttcggggcgt ccggccgttc gcgatcaaca ccaggcatcc gtcgggaacg
    17521 ggcgacttaa gtgatgcgtc gatatccggg ttgtccgtag agttggaaac ccagaggcgg
    17581 cccgcaaaat ccagaaaggg gaaattgggc gtggacattt tcttgccctg cgcttcggtc
    17641 atcaacacgg catgctttcc gtccggggac agagactgga cttcgccgtt gccgatgttg
    17701 gcgacaatgc agttgccgtc cgtgtcgatg cagatgccgt tgggcagacc gcccaattgg
    17761 ccgaaaaaag tcgtccggcc gtccggatca atgcgggcgc agcgtccgtg ggcgtccgcc
    17821 gtgcggacgc cgccgtcatc cagcgccatg acgccttcgg ggcgggtcag tccgctcccg
    17881 agggattgaa tctgccgaag atcgattttc atgactctcc tgctgtttgt cgcgtatgtc
    17941 gggaccctat cacaatttga tggtcgcggg caatcattag tcgccggttc gggctgttct
    18001 ttttcgccgg gaaaaaaccg ccggctgcga aacctgtcgc cggcgccgcg aaggaatgcg
    18061 cctgcatgaa ggaatgtgat tgacaggttc gaatcttctg tctatactcc gcgccatcct
    18121 gacaggaaat aatcgcaagt aaacggattt tccatgttca atcgcatcgt cagcgttgtt
    18181 tttctggtct ttatcgccct gacatccatc ccgttgtttt tgacggctct gctcctgcgt
    18241 ctggcgacct acccctttga ccggcgtttg cggcttttgc acttattcac gtgtttctgg
    18301 gcttcattgt acacctggac aatgccggcc tggcggattt acatcgaagg ccgcgaaaaa
    18361 atccggaaaa acgcgacgta catggttgta tccaatcatc agtcgcagct cgatatcctg
    18421 gttgccttcc ggcttttctt tcactacaag tgggtgtcca agattgaaat attccgtgtg
    18481 ccgctgatcg gctggaatat gcggctgaac cgctatgttc agctcaagcg cggcgacaag
    18541 cagagcatcg aaaaaatgct gagggattgc gcggtgcacc tggaagaggg aagttccatt
    18601 ttcatgtttc cggaaggcac acggtcgccc gacggcgagg tcaaggcctt caaacccggc
    18661 gctttccagc ttgcgctcca aaagaatgtg cccattctgc cgattgtcat cagcgggacc
    18721 aataaggctc ttcccaaata cagcatgaat tttaacggtg tgcagaaaat ttacgtcaag
    18781 gtattggaag aaattccgta tgaggccttc gcgcatcttt cggtcgccca aactgctgaa
    18841 atggttcacg gttttatcac ccgtcaactg cggctgatga aggaatgcac gccggacccg
    18901 gcctgatcgc ttttttcacg aaaagcccgc tcaggaagca agctgtgccg cgcccatttt
    18961 tgcctggcgg atctgcaggc gtcttttttg aagcatttcc ataaattcca tgtctttttg
    19021 aagaaggctg aagcgttcgg gctcttcgat gcgcagacgt tcggtgaacc gggcgacgtc
    19081 ggcgtgaaac aacgccgcga agcgggcaaa ggcgatgctt agaggcgcgg gcgattcatt
    19141 gccccggtcc agcctgccgg ctgcttcgca ggcgatgctt cgcagggcct gccacaggga
    19201 attcaacgcg ccccattcac ccggcagagt tttctccgcg gatgcggagc gctcccgaag
    19261 ggcgattctc agaagatcca gctgcgcggc catgccgccc agaacgggtc ccatcatcac
    19321 cacgtcttcc gtttctccca aagggacgac gatgtcctcc caggcgtttc cctcccggcc
    19381 gaggacggag gccttcggga taaggcagcc gttcagttga atgccgccgt gcggtgaagg
    19441 ctttaaaaag ttcaggggca tcggcggctc tacaaagagg ccctgcgcgc cgcgcgggac
    19501 catgaacgcg gtaaaacctt ttcgcgccga cgtatcgtcg gttacggcga caaccacaaa
    19561 caaatcggca atcggtccgt tggtcaggtg ggttttttcg ccggtgagga ggtaggattc
    19621 cccctgctgt tgcgctttcg tggccagtgc tttcggagaa gcgccgcgtc ccggttcgga
    19681 cacggcaaag ctgatggcgc agtttcccag agctgcggcg cgcagatatt gccggcattg
    19741 ctccgtcgtg ccgaatgttc ccacaacgga gcgggcgatg atctgctgga agatccagga
    19801 cattcccaga cccagattca gtccgctgcg gacaaacgct tctccggtcc gcaaaaggtc
    19861 aagatagccg cagccgtcac cgccgtattc ttccggaagg ccgccccgga acagcccggc
    19921 ctttcccatt ttccgccaga ggtctgaagg aaattcctgc tccgtctgca aatcagcgcg
    19981 gcaggcgacg tgccgcgcgg cgaaatccgc ggccagcgcc ggcatgtcga aaggcttgtc
    20041 cggttgtttc atatgaccag ttcctcgatg aacgtgaaca actggttgac gttgcggcag
    20101 gcgcggactt catggcagtg gcgcaggtag gtccggatct cgctgtcgcc cgtgccccac
    20161 agtttctccg gttcgggatt aagccagatg acccgtcggc agcgctcccg catggcgccc
    20221 agaatcgcgt cttccggatt catgtaattc gaccgtccgt cgccgacgat gatgagcgtc
    20281 gtcttcttcg tcagaagatc catgtagtct cttttaaaat ttctgagcgt ctcgccgtaa
    20341 tcggttggcg ccgagtagtg cacgtccgct tcttccatga cgatgcggat ggcttcggag
    20401 atatcctgct cctcaaagat gccggtgacg ttcgtcaact ggtcgatgaa gacaaaactg
    20461 ttgaccttat cgaagcagtc ctgaagagaa taaaccagat tgagcatgaa gcgggccgcg
    20521 gcccagacgg atccggacac gtcgcatagg gtgacgatcc ggcttttgcg caggcttttc
    20581 cggcggtact tgatgtcgag ggggacgccg tcgtatttgg ccgacgcccg cagcgttttt
    20641 ttcacgtcca gatttccccg gtcgcggacg gcaaagcgcc ggctgacgat gtttttcagt
    20701 ttgcgcacca gccggtcgat ggcttcgcgc atttcctcca cttcctcgcg ggtgaaggac
    20761 gaaaaagcct tttcaccgag gtcgcgcacg cgctgctcgt gcgtttttac ttttttggcg
    20821 ccgccgtcgc ccgcctgcgg ctcgtaaacc agaatggacc gcgcggcctc cactcgctct
    20881 tcaaaatgag ccgcaaggtt atgacggtcc gccggcgtca tgttcaattc attgttttcc
    20941 agaagcgcgg cggcggcgtt tgccgcggca ttgatctgca gcagaacgtt gaagcggctg
    21001 gcgagcggtc cgagattgaa ccgtgcgggc gctgccgcgt catccgattc caaatggatc
    21061 cggcgcattt ccgccagcag ggccatggga tttccggcca gaaaatccac cacggcatca
    21121 tacatcggat tgtcatgcgg ctttttcctg agcaggtccg tcgcttcccg aatgcggtcg
    21181 atctgggagg ctgcctgctg catgtcctcc gcgttgacgc gcatttcgtg aaaaaacaga
    21241 tggtagaggc gttcaaagtg cttcagttcc cgcaggcttt tggcaaaatt ggcccgcagc
    21301 agggccttga actgcagttc atcggtcggg tcgatcagct cgagctgctt gagcgtgtcg
    21361 aggacttcgg aggtggagac gcgcagcccc gcggcccggc agcaggaaac aaattgccca
    21421 atcagtttga ccataaatca ccacggcggg attccggagg cgcacccgca aaagacggct
    21481 gccctgccgg tcgggtcttt acgggctgtt gcccgaatat ccttaatctt ttccgggacg
    21541 ggaagcgtga tgaaggcttc atccggctcc ggcgacgacc cggtcgatgt ttttccgcac
    21601 tttttccaga tccgcctggt atttgcagat gacgttgagg gtttccgaga tgacttccgg
    21661 cgtcagttcg gtaatctgca gcgccagaag cgattgcgcc cagtccaggg tttcggaaat
    21721 actggggctt tttttcagat ccagggtgcg gattttgcgg atcgaatcca ccaggacggc
    21781 gcagagtctt tcctcgatgt ccggaatttt cagacggaca atcgagattt ccgtgtcgcg
    21841 cgccggataa tcgatgtaga gatgcacgca gcggcgcttg agggcgtcgc tcatgtcgcg
    21901 atagttgttg gacgtgagca ggacaaaggg gatccggacg gccgtgcgtg tgcccagctc
    21961 cggaatggaa acctgaaaat cactgagcag ttccagcaga aaggcctcga actccggatc
    22021 ggacttgtcc acttcatcga gcagcagcac aacaggcttc gccgaactga tcgcctgcag
    22081 aagcggacgc ggctggatga agcggtcgga aaagaaagcg tcttcctgag cggcgatggc
    22141 atcgaccgct tcggcaagcg ttgccgtggc gccgatgatg tcgttgatcc ggtccttgac
    22201 catctgtgtg tagagcaact gcttggcgta ttcccattca tagagggctt tggcctcgtc
    22261 caggccttcg tagcactgga gacggatcag ctcccgtccc aggctgcgcg ccaccgcctt
    22321 ggccagctcc gttttgccga cgcccgccgg cccttcgatc aaaacgggct tctgggtcgc
    22381 ctgcgccaga aaaataaccg tggcgatttc ccgggacggg atgtagccgg cttcaatcaa
    22441 tcttttttca acatcagaaa cagttttata agacatggca gacctttctt tggagctctt
    22501 ataacaaaca ttctccgcgg tcaagaataa gggagaaaag gaaacttgcc gcaaggccgc
    22561 gggccgcccc gggcggcccg cggccttgct tcagggcaaa agagtgtttg acttcctgaa
    22621 atgtaatatg gtagtaccca caaaattttt tttaaaattt tgaatgattt caagatgctg
    22681 aattgaaaag ggagtccggc tgatgacgtg ggaagcaatg gcgaatatct ttcaggggat
    22741 cacgacgctg acggagtctt tttcccacaa tccccgaatg gcggcggcca gaataggact
    22801 gattctgctc gggtttctga tgatctacct gggcaaaaag ggatatctgg aagcgctttt
    22861 gatgattccc atgggactgg ggatggccac cgtcaacgcg gccatcatgt tttttgatcc
    22921 tctgaacctg cacggcggag tggggaccct ttttgtggag gcgcaggccg gcgccacagc
    22981 cgacgccgtg aaaaacgcag cggaaatcgt caccattttg cagatcgact ggctccagcc
    23041 catttacacc tttaccttca gcaacggcct gattgcctgt ctggttttta tgggcgtcgg
    23101 cgtgctgttg gacgtgggtt tcgtaatggc ccgtcctttc cagagcatgt tcattgccgt
    23161 ttgcgcggag ctgggaacga ttgttgtttt tcccatcgcc cagtggttcg gctacacgcc
    23221 ccagcaggcc gccgccatct ccatcgtggg cggggccgac ggaccgatgg tcctgttcac
    23281 atcgcttagg ctggcgccgg agctttttgt tcccattgcc atcatcgctt acctttattt
    23341 aggctttgcc tacggcggct atccatacct gatcaggctt ctggtgccca aaaagcttct
    23401 ggggatcaag cccgcgccgg cgaaaaagaa gcgcagcatc acctccggcg agaaaatgct
    23461 ttttgccgtt gtcgcctgcg tctttctgtc cttcctgctg ccggtggcgg ccccgttgat
    23521 cttctccctg tttttcggcg tcgtcattcg ggagtccggc atcaaggaat tcatcgatct
    23581 tttgcagggt cccatccttt acgggtccac gatgtttctg ggcctgatgc tgggcgtttt
    23641 gtgcgacgcc aacacgatca tggacccgaa ggtattgccc ctgctgcttc tggggatact
    23701 ggccctctta atatccggaa tcggcgggat tctggccggc tacgtcctgt atttcatgac
    23761 gcggggaaaa ttcaatcccg ccatcggcat cgccggcgtg agctgcgtgc cgacgacggc
    23821 caaagtggcg cagaaggtgg tttccaaagt caaccgcggg gccattattc tgccggacgc
    23881 gctgggcgcc aatgtcagcg gcgtgatcac cacggcgatt atcgccggca ttttctgcag
    23941 cctgctggcg aagtaaagga gaagcattat gagcggatgg cctgaagctt tactcaccta
    24001 tggactgacg atcgtcattg ccttcggcat cgccggcatg attcacctga tggtcgttct
    24061 gatggaaaaa tttcccccca aaaaagacaa ctaacggcgt gcaaacgccg tcttcccatc
    24121 ggatattcct tccttgaaat tcatccggta ttctggttta ttgccgtaag cagaggtggt
    24181 tcggtctatg acaacaaagt ccccatccat ggagaaaagc gattcctggg tttcatcggc
    24241 gctgaaagtc aatctggagc gcacggcggc ggaggttcag attccgctgc agtacgcgcc
    24301 gctgcttgag attgtcaaag accactacgg cctcaggaaa aagaccagcg agcttttgac
    24361 ggagctcaat catccctatg tcaactggga ctttgtcctt cgggagctca agtccatctc
    24421 catcggcaat tttcacctct acagccgcca tgcggacggg cttgccggtt tatggatcct
    24481 gctgaccatt tatttcgata ttctgaaatc cgcggccggc gaagacgtga aggacagcgc
    24541 gctgcactac ctgttcgact atgcggacac ggtgcttctg cacagcgagg aatttctgga
    24601 gaggaacctg ccgctgtttc cggagatgat cgagtccctg gcagcgatgg caaagggcga
    24661 tccggcgctg ttcaggaaat gctcttccta cctgaaaaga atcatccgga cggcggcgga
    24721 gaagaacgtg gacctggcca cgccggcgtt tgacgcgctt ttgtacaaaa tgttccgtgc
    24781 gacctacgcc ttctggctga gtcagccgga ccccgcgctg tggctgattg ccgaacgccg
    24841 gggggatgcc ccgcccaacg aaacccccta tctggagctg atccgtccgc tcagccatca
    24901 ccggttccgc aggctgatgc tggagctcga agcgcttcgt ccgcgtgaag gggagataaa
    24961 cggcgcgcgc attcgggact ttctggccct gccggattat tttcagattc tcgacaccta
    25021 cctgcgggtg gcctccgcgc tggaaaaaac cgaagcctac gcgggccgcc acctcgtaaa
    25081 gctcgatttc cttttcggca tcatgacggc gccgggcctc agggacattc atgccggcgc
    25141 catgcgggaa atcaacgcgt cgctcaggct ggtttttcag gaggaaaaaa aagagaatct
    25201 ggatattttc gtgaggaaga tcttcggctt cctgaaaaga aatacgtcgc gcggcgaatt
    25261 ccgcagcgcg gggatcgact gcattctcac ggcggcccgg gaagtctttg cgcaaaacga
    25321 ccaccccctg gtggaagcgt tcatggacga actgattgcc tacggttttg aatatccgga
    25381 gattcaggga gcaacggccg aatggcaggt gcgggtcaac cccgagcatg tccgaatgat
    25441 ccgcgcctgg ctggaaatga tcgccctgaa gccgcgctgg acgaaaaggc tcatttccgc
    25501 cctgatcatt cacctgaaaa ccggcgggat tttcattcgg gatacggacc tgattcagcg
    25561 cgacatttcc cgcctgctca acacggacat tgcgccggcc tacagcctga tcaagcagct
    25621 gttgcgcctg tttcccgtgt acttcagcga aatcggcgcg gagggagaac tgagggacct
    25681 caccacccgt gtggacgaac tgtcctgccg caacgaccgg ctggtcgatt ttttccgcaa
    25741 gcagtcccac gtggagagca acagcctgct ggttgaattt atcgaggata tgttccgctt
    25801 ctgggtttcc ggggacaaaa aatccatccg caagcatgtt cccgatgaaa ttttcgatca
    25861 ggtggccggc gagggtcctt attttgacgg aatgcaccgt gcgctgcttt acctctttcc
    25921 ccttgtcgga aatcagccgc agaactttct ggagtgggac ccggagagaa ttgccgcgga
    25981 gatttcccgg attcagaatg tttccgaaac ggaccgggag cgcgcgtcgc tgatggtccg
    26041 catttaccag ttgatctaca agaaatatca tccccgctat catgatctgc tcacggacct
    26101 ggagaccgcc ggcgtttttg ccgcgccgga cctttcgtcg ctgaagcgat cgctggccga
    26161 aaaacaccat taccgaagcc tgacgctcat tctgaaatgc ctcggttcgc tcaaggcccg
    26221 cattctctcc gggaaaaaaa cccggtcgtt tgaaaacatt tactacaaaa ggcacattgc
    26281 cgcgggcatt ccgtccatgt acggaaccta ccacgaggag aaattcgacg cgctggggct
    26341 ttccctgcgc ctggaaagcc tcgcgggcat gctgtttgaa gagctgatcc agtccctgaa
    26401 tctgcagttc atcaccaagc gcaccatcat caggattcac acctatctgt ggacctatct
    26461 gaccgcgctg gatctggagg gaatctccac cgaaggactg gtggccaagg tgcggttcat
    26521 caccagcgcg ctgcccatca agcagttttc catggagcag tacctggata ttttccggtt
    26581 catctccagg ggcattcagg acgtcattcg cgactactac atcgacgcgc acagcgtcaa
    26641 tctgccggtg attctccgtc agcttcaggc gcgggcggat cagacgggcg ccgccgccgg
    26701 cggggccctt ccggatcaat tcatctatca gcagtcggag aattttctgc gcggcgtgat
    26761 ctcttccgcc tttggactgc aggtgctgga taattttgtc cacaccgtca tcaaaacgct
    26821 caacgcggag ctggaaaaat tcaaggacaa caaaagaatc gtcaaccttc tgatggatta
    26881 caatccggac cttgccgtct cgcccctcta cggcaggaat ccgaaaatgg acaaccagat
    26941 tctgctgggc aacaagggct atttcctcaa gaagctcgtc tccttcgggt ttcaggtgcc
    27001 gccgggcttc atcatcacca cggaagtgtt tcggggattt gacgcggtct acgggtacaa
    27061 gtacattttc cgggacctcg cccggcgggt caacagggaa atcgccgcgc tggagcggaa
    27121 aaccggccgg cgtttcggcg accccgttca tccgcttttg ttgtcggtgc gaagcggagc
    27181 gacggtttcc ctgcccggca tgatgcgctc ttttttaaac gtcggcatca acgccgcgat
    27241 tgccgaaaac ctcagcgcca gaaaggattt ccagtgggcg gcctgggatt cctaccgccg
    27301 ctttcttcag acctggggaa tgttccaggg gctgagccgg gatttcttcg acgccatcat
    27361 ggacggattc aagcagaagc acggcgtgca gagaaaaatt cagtttccgc ccgagcggat
    27421 gaggcagatc gcccttgcct accgggaggg cattctggac agcggcattc cgctgtcgga
    27481 taatccgctt cgccagctcc gccacgcggt tttgcaggtt tttgattcct ggtatgccga
    27541 gcaggcgcgc atctaccgcc accagatgca tttgtccgat gcgtggggaa ccgccgtcat
    27601 cgtacaggcg atggtcttcg gcaacctcca tgaacggtcc ggttcgggtg tgatctttac
    27661 ccgcgatccg aaaagcgctt cctccgacgt ggccctttac ggggatttca tcttcggcgt
    27721 tcagggggac gatatcgttt cggggctggt cgaaacgtat cccatctccg agcgccagcg
    27781 gcttgccgaa cagcgcaaca cgggaatttc cctggaagcc gactttccgg ccatctatgc
    27841 cgaactggtc aaaaccgcgg aaacgctgat ctacgaaaag ggcctgaacc accaggaaat
    27901 tgaattcact tttgaaggcc cggaaaaaga acagcttttt ctgctgcaga cgcgggacat
    27961 ggaccagacc aaggccaaaa gcctgcggcg ctttcgggag acggaccttc tgcacgcctc
    28021 catgctggga acgggaatcg gcgtgagcgg cggcgccctt tgcggccggg ccgtttattc
    28081 cgaaaaggac attcagcagt ttcgcgcccg ggagcccaaa accccgctca ttctgatccg
    28141 ccccgatacg gttcccgacg atgtcggcgt ccttctgcag gtggagggac tgctcaccgc
    28201 caagggcgga gcgacctccc atgccgccgt gaccattccg caactgggca aagtcggagt
    28261 ggtggggctg agcaagctga aggtctatga aatggaaggc tatgcgacgc tggagggccg
    28321 caccattcaa agcggggatt ttatcggcat cgacggctgg agcggcgttc tgtatctcgg
    28381 aaagcacgac ctggaatcga cccccagcgc aaccctcacg ctgtagggct ctgcgcgtca
    28441 aaaaagcaga acttttcttc cccccgggct tttttcacct gatacatggc cagatcggca
    28501 tggtggatca gaacgtccgc gctttcgccg tgctcgggat agagcgcgac gccgacgctg
    28561 aaaccgactt gaacttccag gggaggcgaa ccgacctgaa ggcatggtct gaatttttca
    28621 aaaagaaggc ggatgaagtt ttcgatgtcg gttctttgtg agacatccgt cagcagaatc
    28681 aaaaactcgt cgccgccgat tcgggcaagc gtgtcgtttt ctcggcggac ggcgcttcgc
    28741 aggcggtcgg cgacctgcgc cagcagttga tctccggttt tgtggccgta agcatcgttg
    28801 atgtccttga atttatccag atcgacaaac agaagggcca gtcggcagga ccttcgcttg
    28861 gcggaaacca tggcctgctc cagccggtcc cacatgagct tccggttcgg cagtccggtc
    28921 aggggatcgc tgtaggcgag ctgcatgacc cggccttccg tgaggtccac gaaatttccc
    28981 aggaacgccc gcttcccgtt catgtgaatg acgttgaccg atcccagaat ccacttgatg
    29041 gcgtcctttc gggtcagtac gcgaaaaatg aaaaaagagt ccacctgctc ctccagcgag
    29101 gaccggacgc tgttttgcac atggtcccgg tcttccggaa agaccatcgg caaaggatcg
    29161 attccccgca attcgctcga agaacagccg caaatgtcgc agaaggtctt gttggaaaaa
    29221 atcagccggc cgccctggac aatgcagatt ccgaaagggg acatattcag cagaaggcgg
    29281 atgaaggaat tcagctcgtg gacctgccgg acggtcgcgt cgaactggag tttcaggttg
    29341 cggttttccg cgagagcttt ttccagcgaa cactgaattt cgaatgaacc attccggggc
    29401 atgttccctc cgagtgcgcg gctgaatttg ttttcggatc gcttttcaag cgactgacgc
    29461 gcgcgttttt gcgcaaaccc cgaacaaaag tgaatcgccg aggaggagca ttcgcatgga
    29521 ggaccctcgt tttccctttc cgggaaaacg agggtgtccg ggtttgaatc agccgggagc
    29581 gggcccaaac ccgctccgat tcataacggc ttattgaaga tttttgtgga tatagacggt
    29641 caggtctccc atccggttgg tgtagctgcc gtattcattg tcgtaccagc cgaaaatctt
    29701 ggcatggacc accggaacct gcagcaattt ttcgccgagg cttttaacga gatccgccgg
    29761 cagattcgga atatgagaca aatcaaccgg tatgaaagcc gtccgggtgt gattgaattg
    29821 tccttcgatg atgaccgccg cgtcttcgcc gatcagatcg gtggagacgt tctgctgcat
    29881 ggtgaaacgg acaagcgggt tgttcccggc ggccttcgcg taaatctcat tgagcttttt
    29941 ggtgtcgagg cccgccgccg cggctttgtc gttgttccgg gcctggaacg tcgcgttgag
    30001 cacgatgagc gaaagggtgt tggtcggaac ccggatcgaa tcgcccatga agccgatgtt
    30061 tttgacttcc ggaataactt cggccagggc cttggcggcg ccggtcgaag tcaaaatgat
    30121 attgttcaga atgctgcgtg ttttgcggat gtctttttcg cccgctttgg gcagcttgtc
    30181 caggacgctc tgcgtgttgg tcacggcgtg aatcgtggac atggacgccg tcagaatggt
    30241 gctggtttcc tcgttgtcca gaagcggttt gaccatgtgc gcaagccccg tcgtggtgca
    30301 ggacgcggcg gaaaccagca ggtgcttctt gggattaaac gccgtatggt tgatgccgta
    30361 gatcagtgtg gtggtgtctt ccggcgttgc aagggccttg tttttgatct tgaagggcgc
    30421 ggaatgaatg accactttcg cgccgccgtt taagtgaccc cggatcgagc ctttcttgtc
    30481 gtccacggga accgtcggat ccttgaagct gccgctgcag tccacgacga tgtccgcccc
    30541 gtactgacgc cagggaatgt tcagggggtt gcgctgttcc cgcagaatcg tcaccggaat
    30601 gccgtcgatg agcatcttgc cgtttttatc gtccgtaatc tgaatgcagg gctgcgcttt
    30661 gatgccgtac aggaaacggt gcagcaggcc gtaggtcgcg tccgcctcga tcaggcgcgc
    30721 aatggtttcc atccccatgc cgatgtcccg gccctggttg acgataattt ccttgaagta
    30781 ctttctgctg atgtggtgcc acagggtcag tttaccgatt cgccccagac tgttgatgcc
    30841 gagcaccggc accctgcttt catttgcact gctgttggtc atgatatatt tcctccttta
    30901 cccctcaaga cggcgggtat gattttgctg ttcggcggag ttgttcagga gcatagaacc
    30961 ccgatgatgg cgatggaaga caggctctaa atgacaagac gcaaattcgg agtccataat
    31021 tttaaagagg tcgtttcggg aagaccggaa ctctcttata ggaaagggcg gccaaagtca
    31081 agttcttata aaaacgctaa taatattggg atgatataat ttggagccga tgcgcaatga
    31141 aacaggattc acgagcagca gccgccgctt ttttgggctc ccgtttcatc cgtccgcggt
    31201 ccgcagcagc attcgccgcc gtttcgtccc agcaggcttc cgatgacggc ggccgcgcag
    31261 ccgactcccg attgaacgct gattgcgccc gacgggcagt tttgacggca cgcgccgcat
    31321 tccatgcacg cgtcgaaatt tataatcatc gcgtttttga cattcatttc aaatacatgg
    31381 tgtgggcaga cctcgatgca catgccgcag ccggtgcatt ttttttcgtc catttgaagg
    31441 gttgcgacac ctttcagata cgtgagattt cccatttttc ctcccgcgat gctgcgttgc
    31501 gatccctttg cacgctgcag aacaggggct tgcagcccct tgttctgatg agccggcaca
    31561 ggacgcctac aaaacaacca gcgatgcaat ccagccggcc agtccgaaaa cgcccgccga
    31621 tatttccagc ggcaaggcca gccgcatttc tttttttacg ccggagagcg atgtataagt
    31681 cgaacagccg gtaaaattca tggccagata agccgatacg gccggaatga tcagcagcca
    31741 ggacaaagcc tccagttttc cggcgcctga ttgcagatcc atgccgcgca aaaaaatcag
    31801 tccgagggcg atgaccagtc cgatgatgaa gcccttgacg gaaaaagcgc gtccgggaat
    31861 gcagggcagc agaagcggat taagcaccgc cccgcccagg acggcgcaaa agagcgccgc
    31921 gacggcaaac cctccaatct cctgaatgtt gatcagaaat ccattgggcc ccagcaagcc
    31981 ggacagcaaa agcagaaccg aagacaccag cagaaaagat ttggccgtgg acaaaagttc
    32041 gatgggaatc aatactgtcc gctctttcat ggagaatgtt atttccctca tttgatccgt
    32101 ctttttcatt ttgccgtcga gataagcggg caggtcggat gcgcggacgg ggccgtaata
    32161 aacctgaaag cccgttgatt gcctgacccg atgcgcggac acgccggggg ctcccaattg
    32221 aggcaaaatg agctttctgt ggctgaccaa ttttttaaga ccgcttgttt ctattctccg
    32281 gagaagttcc tgcgtgccga aagtgccttt tcccgcggca caccatacat tgattccttt
    32341 agtgtccagg accagaatcc aggcattcct attcggcagg gcttctctga ggcagtcaaa
    32401 gctcattttg taattggcgg tgaccagcac cggcgactgg tcatccggat ttcccaatgc
    32461 gtaaaggccg ggatcgatgg cgtaattcat gcgtccggcg cccaggcggg ctttgattgt
    32521 accccatcgg tcctgccgtg ttaacaggga tgatactttc ggcaaatcac ccgcgggcgt
    32581 ggaaaacgat cctgagacaa aggactgatc cagccgcgca atgggatcgt ccgcggccgg
    32641 cacggcgggg ccgccacagc cgcaaccggc cggctcctga agcaccggaa attcaaatcc
    32701 attttttatg tccatcgctt ctctcaacaa attattgaca accattacag acgccggccg
    32761 cgtttttgat cgcattgatc catatctgca cggcctgcaa tatattttcc tcttttccgg
    32821 caggcagatt ggcggccacc ttattgaaaa attcctcaag acagagctga acctgccggt
    32881 gggtttcctc gccgctcttc gtgagcgtca gcccgaagat tctggaatcg gaggctgatt
    32941 tttccctggt taaaagtccc ttctcgagaa gaggattgag cagccttgtt gtcgtgcttt
    33001 tttccacggc cagaagggag tgcaaatcgg aaatcctgag atatttgttt ttggcggcgg
    33061 cattcaggat gataaactga tgaaaagtca ccccgccgca gaaggcggtg tcctgccgga
    33121 agcatcttgt cgcctgcgcc agaccgacaa acacgtctat taatttggct ggatgaccct
    33181 ctttcttttt cataatggtt gtaatataca actatgtccg gctttgtcaa ggggggaatt
    33241 gaaaagtgaa gagcaatgag caatgagtga cgaggaaaat gggaaatggg agaagggctc
    33301 gaagctcatg gctgatagaa gcaaaaagtg aatggtttat agtggattgt ggatggtaaa
    33361 aaaaacaggt gatgggtaat aggggtttga ataggaaata aaaaatgtta ttttcatgca
    33421 cgccgggtca atgccctgcg atcaacggtt caaaaatgaa aaatgatggg aaagggattg
    33481 aatatgaaaa cttattctga tgcggccgag gaaaaattca tcgaagggta taattgcgcc
    33541 cagtcggtcc tgttttcctt ttgcgaggag ctggggctgg atgcggacac cgctttgaag
    33601 atttcctgcg gattcggcgg cggcatggga cggaagggag aggtgtgcgg ggctgtgacc
    33661 ggcggcgtga tggttctcgg gttgaaatac ggcaggggga taaaagagca gcgggagcaa
    33721 acgtatgcct cgtatgtcaa aatacgagag ttgttcgacc gtttttccgc caggcactcc
    33781 acctgtttgt gccgcgaact gcttggcggc tgcaatctgg ccacgccgga aggacaggcg
    33841 tatttcaagg aaaatgattg tctccggctt gtctgccggc cctgcgttcg cgatgccgcc
    33901 gaactggttt gcgacctgct cgccgagggg taatgttctg caaaacagcc gccgcgggcg
    33961 cgattccggg cgtgaccggg gccgcctcaa ccggatcatt caagaccgga ttattgagtt
    34021 gacacgggag gttcctttcc ctatactccc ggccagttaa aatgaaggag caggaaaaca
    34081 tatgacgatc aacatcaaag ccgaagaaca tattatgctc attgagatcg accggccgaa
    34141 gaaatacaac gccatgaccc gggaaatgta ctcccaaatg gcgcaggcgt attatcaact
    34201 ggatcaggac gaccattacc gggtaggcgt ggtttatgcc aatggtcctc atttcacatc
    34261 ggggcttgaa ctgaccgact ggtcggatgt ttttaccgga ggagcgggat ttccgctggc
    34321 cgaaaaaaat gaaattgacc ctttttacat gatgagcgac gcccgatgcc gcaaaccgat
    34381 catatttgcc gtgcagggat attgcttcac atggggcgtt gagacgatgc tcaacgcgga
    34441 catccgtgtg gcggcatccg atacgcggtt tggcatgctg gaagtgcgcc ggggcttttt
    34501 cccgtgcggc ggggcgacac tgaggctgcc caaagaaatg ggccgggcca atgcgatgcg
    34561 ctacctgctg accggtgatg aatggactgc cgaggaggct taccggacgg ggctggtgca
    34621 gcatgtgacc gcgccgggca agcagctgga aaaggcgctg gaattggccc gtcgtgtggc
    34681 gacagccgcg ccgctgggag tccgctatat cctgaaaaac agccgcacgg cggagctgca
    34741 gggagaaaag gcggccaggc aggatatctt tcaggatgtg gccggggtta tgaaaagtga
    34801 agatatgaga gaaggtctgc aatctttcat tgaacggcgc gccgccgtat ttaagggaaa
    34861 ataattgtta attttaaaca ggaggaagtg aatatggcac acagcaactg gttgtatcag
    34921 acgcgggaca tcatgttcca gatcaaagaa tggcttggag tggaaaagct cctgggccta
    34981 gatgcttata aggattacta cgggatggac gacatcgaca gtttcctcga tgtcaacttc
    35041 aaagtctgcc gcgacgtgat gtgcccggcc aacaaagacg ccgatgaacc cggcgcgaaa
    35101 tttgcgggcg gcaacgaaca tgcggtgacg acgcccgact cgttcaagaa tgtttataaa
    35161 accattatgg acgcggagct gggcccccag ttcggctacc gcggcgaagg caaaataccg
    35221 ctgtgctggt atgctccgat tctggagatg cagtccgccg cgtcgccgtc gatcgtcatg
    35281 ttctggtgtc tgacgcaggg cgcgatcacc gtgcttcatg aatttgccac gcaaaaacag
    35341 caggacctgt ttatccccaa actgcgcaca ggtgaatggg gtggcaccat ggggcttacc
    35401 gaaccgggcg caggctccga agtcggcgcg gtgcagagca aggcgtttcc caccgacacg
    35461 cccggcaagt acaaactcac cggaacgaaa tgcttcatct cttccggtga ccatgacctg
    35521 gccgaaaaca tcatccatct gatgctggcc aaaacgccgg gcgccaagga aggcaccgcg
    35581 ggcatatcgc ttttcatcgt gcccaagttc tgggtcaacg aggacggctc gctgggcgcc
    35641 tggaatgacg tcaccaccat gggcatcgag cacaagctgg gcattcacgg ctcgacgacc
    35701 gccaccctct ccatgggtga aaacgacaac tgctacggct ggatggtggg cgaagcggaa
    35761 gtgaaagacg gacgcggcat cggcatgaag cagatgtttg cgtatatgaa cgaggaacgc
    35821 ctgaatacgg gcttgttctc gctgggctgc atcggatctg cctattacgc cgcgctggat
    35881 tatgccaagc tgcgcgtgca gagcaagcat tccaccaatc ccaagggccc cagcgtccgg
    35941 atcatcgaac acgaagacgt ccgccgcatg cttttgtccc agaagtccgt tctggaagcc
    36001 tgccgggcgc tcatttacaa gtcctatctc taccgcgacc tggccgtcga tgccgctacg
    36061 cccgaagaga gggaatatta tgatgacatg tttgcgatca acaaccccat gtgcaaggcc
    36121 tatacatccg atatggcgag gctcaccacc ggcgacgcca ttcagtgcca cggcggctac
    36181 ggcttcacgg aggagtatgc tcccgcggag ctgtacaggg atgttgtgat ttacggcatc
    36241 tgggaaggca ccaacttcat ccaggcgcag gattataccg gccgcaaatt caccatgaag
    36301 gacggcgcgc cgttcaaaaa gtgggtggcc gaaatcgacg actttgtcgc caacaaaaag
    36361 acggaagcat tcgcggcgga attcgcaatg atggcggaag ccatgacggc tttcaaagag
    36421 atcgtcaaca tgaacgacgc ctggacgaag ggcgaccgcc agatgaagca gcttttcgcc
    36481 acgcggaccc tgcatgcagg cgccagagtt tactgcggca tgctgctgct cgaccaggcg
    36541 ctgctggcgg cggcaaagat gaaagagaaa ggcgagatcg acgccgattt ctacaagggc
    36601 aaaatcgcca cggcccggtt ctatgtcatg aatcatgttc ccgacatctt cggatatctg
    36661 aagaccatga agtacggcga ccgcagcgcg atcgatatcc ccgaagaaag tttcatgtag
    36721 ggcgtaagga acggtcgcga ccgctccagg cttttag
//
LOCUS       DAOPAF010000012        32828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1497_length_32828_cov_54.484972, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000012 DAOPAF010000000
VERSION     DAOPAF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 32828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32828
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_32828_cov_54.484972"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLQ77_02440"
     CDS             <1..245
                     /locus_tag="PLQ77_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09570.1"
                     /translation="DEWEFNFKADAFYFQSLRMPTADWPETQEDTEGSLLERIYRIEN
                     AVKTIDQLYESFLAIRFSPEWAKNEKPRLATWLRKE"
     gene            359..649
                     /locus_tag="PLQ77_02445"
     CDS             359..649
                     /locus_tag="PLQ77_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09571.1"
                     /translation="MDQKQIFKQMMDFNKQAFDNSFSVMSELQEQTEKLMLNFLEKAE
                     WIPEDGKKAMQDWIVNNKKSRNDFKAAADDSYRKVAELFEQAEKQQTPKKKK"
     gene            676..939
                     /locus_tag="PLQ77_02450"
     CDS             676..939
                     /locus_tag="PLQ77_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09572.1"
                     /translation="MDPKQVAKQMIVFNKTAFDNNFRAMQALQEQTERLVGRFWEKSP
                     MFPEEGKKAISDWMSAYKKGCDDFKSLVDDNFKKVEDFFKETK"
     gene            977..2551
                     /locus_tag="PLQ77_02455"
     CDS             977..2551
                     /locus_tag="PLQ77_02455"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL09573.1"
                     /translation="MDHPHIVERMTRPDFYPHRPQKVEAVQTHISYVFIAGDYVYKVK
                     KPVNFGFLDFTTLEKRKFYCEEELRLNRRLAPSIYLDVAPIGRDDAGRLTLGKTANIV
                     EYAVLMNKLPLDKMLKILLARGKADAGIMDAVAKKIARFHQEAQTGGRIDEMGSIGVI
                     RHNHEENFAQTEKYIDVTLSAFQHGFLKSYAEKFLAASEALLAKRVAEHKIRDCHGDL
                     HLEHICVADEIIVFDCIEFNERFRFADVAAEVAFLNMDLDYNGYFSQSADFTNSYLKY
                     SHDEDLRALLNFYRCYYAFVRGKVTSFRLDQKELPQAEREEIRRIASKYFDLACAYAS
                     RLEKPALILTAGLIGSGKSYQARNLADRFGTEVIRSDVLRKELLRIAPTEKHHEAFGQ
                     GIYSGDITRKTYEKAVELAAEKIGRGIPVILDASFRCRADRTMAADMARRLGVPFYVI
                     ECTCPDDVVKTRLEKRMQDRDNPSDGRWEILEEQKKQYEAVTEIPRSCLFHIDTSDNP
                     ENVRRQIVSSIKMAEL"
     gene            complement(2695..3477)
                     /locus_tag="PLQ77_02460"
     CDS             complement(2695..3477)
                     /locus_tag="PLQ77_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPL09574.1"
                     /translation="MKLRHASVILAMVLICTGCSFNMAAIKSFFVKSDPVRSTPEGLY
                     SRGTADYQNGSYKKAREYFTRLKEEYPLHNLAIMAELGIADSFYSVKEYGEAELAYRD
                     FVSLYPTNENIPYALYQTGMCHFVQIEAIDRDQTETIKARREFERLIARYPQSKFSIL
                     AEKMVRECKLKLAEHEFYVGNFYFKNKKYNAALKRFEGIARDYAGVGLDLKVEAYIAE
                     TRDRLAKEEREEKLKEDKEKAKAAKKEEKEKNKIKEKEPTNS"
     gene            complement(3537..3866)
                     /gene="trxA"
                     /locus_tag="PLQ77_02465"
     CDS             complement(3537..3866)
                     /gene="trxA"
                     /locus_tag="PLQ77_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPL09575.1"
                     /translation="MSGQFLGVATDANFEQEVLKSDKPVLIDFWAPWCGPCKAIGPIV
                     EEIAGEYSDRVKVMKLNVDESQKSAVAFGVRSIPTLILFKDGKVLDTLVGMTPKEKLE
                     EFVKKSL"
     gene            4017..4730
                     /locus_tag="PLQ77_02470"
     CDS             4017..4730
                     /locus_tag="PLQ77_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPL09576.1"
                     /translation="MKRLGVLSGTFMLEKSDWIRKGKRQRIHNVYGEAAAIETDSIVF
                     ILRHGDDPNDYILPHRINHHANLQALQDLNVTEVIGINSTGSLKTKLRPGMIVIPDDF
                     ITFAATPSIYHNKAVHITPSLNEAVRQKLITAAHDCNLDVVETGTYWQVAGPRLETRA
                     EIRMMSHFADVVGMTMAHEAVAALELGLPYASACSIDNLGNGLLEKPLSLEEIIAGTR
                     KNADLMIRLLEEYIEGVDK"
     gene            complement(4771..5721)
                     /locus_tag="PLQ77_02475"
     CDS             complement(4771..5721)
                     /locus_tag="PLQ77_02475"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPL09577.1"
                     /translation="METRRDFIKKSVVVCAALSLPLPWAANAQTVQREFKTRDPKRAL
                     VVSYSQSGFSARYGKLIACLLKDQGLTVEWADMRRFDPKRLPDYDLILVGSPVFYYDI
                     PENVADWLSSAPKLTGIPVAAFVSFGGPEGNQHNALCHALHLLAEKEAVPVGMEAFRS
                     IPAYPASWDSANQRSGEHLPDEATYNRVREFAKQIQERVRQGQSLAYSSEIALREIVR
                     MLPLVWLNKKAVGKHTVDGAKCIGCRTCVKMCPVDAIHPERQFVDHDKCIACFGCLNN
                     CPADAVVVEYRGNRLYGFPEYLKRRKITILEPPEFQHCKL"
     gene            complement(5753..7648)
                     /locus_tag="PLQ77_02480"
     CDS             complement(5753..7648)
                     /locus_tag="PLQ77_02480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL09578.1"
                     /translation="MKEAVSNMILNWQDTYKERLTDARSALSQIRRGARIFIASACGE
                     PQLLVKTLLDMAQNFADVEVIHFLDLGLTDYTSEIYTEHFRHNALFIGANARAAIKEG
                     HADYTPIFLSEVPLLMKRGSMPIDVALITVSPPDMNGYVSLGISVDITKTAASVARYV
                     VAEVNPNMPRTLGDSFIHVSEISAFVENDAPLLEFEQKAPDDIAQSIGRLIADLIDNE
                     STIQTGVGKIPNSVFPYLANKKDLGVHTETFTDGLIDLIESGVVTCRKKSLHPGKIVA
                     AFCMGTRRLYDYVHNNPLFEFRPCQYVNDPYVIAQNDRMVSINSALTVDLTGQVCSDS
                     LGFEFYSGIGGQVDFVRGSAMSNRGKSIMVLPSTTDNGAISRIVPYPSAGSGVVVTRG
                     DIHYVVTEYGIAYVHGKSIRDRAMMLINIAHPKFRDELLEAAKRQGYIYRDQTLPVVL
                     YPKEYEITWVDKKGTSLFFRPVKATDERAIQELIYDLPPQDVYTRFFHNLKSFSHKVA
                     MPMAAIDYDDKMAIVAVIGKEEPEGREKIVAIGNYANNPNTRYAEVAFTTHQDWQDRG
                     IGTFLLQYLIRIAKGKNIEGFTADVLSRNLPMMHVFSKCGYPMTTHLDMGVYELKINF
                     GGEEKKE"
     gene            complement(8000..8434)
                     /locus_tag="PLQ77_02485"
     CDS             complement(8000..8434)
                     /locus_tag="PLQ77_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09579.1"
                     /translation="MAHQDKGKYFKKHPEGTTVSDKLKEAVQKSAKDNNVSCKAAEKI
                     AQSTGFSLGEVGVAIDMLNINIAQCQLGLFGLDGKQKKVLPAPSVAPDLDAAIRKALV
                     NGRLSCAAAWEIAARLGIKRMEVCAACENLKIKVKPCQLGAF"
     gene            complement(8600..9055)
                     /locus_tag="PLQ77_02490"
     CDS             complement(8600..9055)
                     /locus_tag="PLQ77_02490"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPL09580.1"
                     /translation="MIAYQLCVFAEDKPGQLANVTSVLAKADISIRATTISTAGTSGV
                     INLIVDDPKSAAKALANAGLTVHLKNVLAVLIPDRPGGLDKLMQLLYREGININNACG
                     FVLECAEKAVFVVDVDQIEKTEKLVEKNGFKTLDTEALSAVEPFHYMKY"
     gene            complement(9118..10017)
                     /locus_tag="PLQ77_02495"
     CDS             complement(9118..10017)
                     /locus_tag="PLQ77_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09581.1"
                     /translation="MKDRQFGRVRFIRGENGGKYPFSHSLYLQGDRERVVIDPASSLE
                     KLTRLKEEGVDGVWLSHWHEDHIRYMNLFEGCPVWVSERDFPPFTDLETFLDWYGIED
                     RGARYYWKKEMLETFDYRPRQTACFLQDDKRMDLGGLTVQVIPTPGHTPGHLSFFFPE
                     EDLLFLGDYDLTSFGPWYGDVLSDIAHTIASIHKLKAIPARRWVAAHNHGIYESNPGP
                     LWDQYEKIIYERERKLLAFLDRPKTPREICSAWLIYGRPREPKEMYEFNERVLIGKHL
                     DYLRQRGKAVLEDGRWRKVESEI"
     gene            10252..10881
                     /locus_tag="PLQ77_02500"
     CDS             10252..10881
                     /locus_tag="PLQ77_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPL09582.1"
                     /translation="MIKALFWDNDGILVDTEGIFFRANQETLADVGISLQWEQFEEIS
                     LVRGGSVLQLAEKPQTEAFYKLRDRRDALYADLLTREPLVMEGVREVLEALKGKYVMG
                     IVTSSGQEHFEIIHSRSGLLPYFQFALTMKDCAHFKPHPEPYLKAIALSGCQPEECLA
                     VEDSPRGLASAVNAGIRCMVVPSRFTRGGDFTGAFRILNNMRELPQALD"
     gene            complement(10924..12555)
                     /locus_tag="PLQ77_02505"
     CDS             complement(10924..12555)
                     /locus_tag="PLQ77_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPL09583.1"
                     /translation="MPITEILTRNASLYCEETSLVEINPQIKEVKASWKDYELVETNP
                     ENRYRREMTWREFDDKANRLANFLLQRGLEKGRKVGILLMNCLEWLPIYFGILKTGAI
                     AVPLNYRYTEEEIKYCLDLADCDVLIFGPEFTQRIEAIREQMPKVKLRLFVGQDLPAF
                     AESYYALTACCSSVAPRVPLSDADDAAIYFSSGTTGFPKAILHHHRSLMMACEVEMNH
                     HRQTREDNFLCIPPLYHTGAKMHWFGSFLAGSRAVLLRGVKPEWVFRAVSEEKITIVW
                     LLVPWAQDILDAVERGDLKLKDYQLDQWRLMHIGAQPVPPSLVRRWREHFPTHDYDTN
                     YGLSESIGPGCVHLGLENIHKVGAIGKPGYQWEIRIVDENGAPVKQGDVGELCVKGDG
                     VMKGYYKNPEATAEILKDGWLFTGDMARMDEDGFVYLVDRKKDVIITGGENIYPVQIE
                     DFLRTHEAIKDAAVIGLPDHRLGEIVAAVIELKPGFAGSEAEMAKFCEALPRYKRPRK
                     IFFEQVPRNPTGKIEKPKLREKYYAGSLVEAETEG"
     gene            complement(12728..12877)
                     /locus_tag="PLQ77_02510"
     CDS             complement(12728..12877)
                     /locus_tag="PLQ77_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09584.1"
                     /translation="MQGHIIATGITFGTALAITISYSYSHSVLWAIFHGILSWVYVIY
                     HVLFH"
     gene            13065..13334
                     /locus_tag="PLQ77_02515"
     CDS             13065..13334
                     /locus_tag="PLQ77_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09585.1"
                     /translation="MRDQLENTCYEKDCAAQEDMAAAGNREKQLTNTIAALRNELERR
                     DIDRQRDMQTMARSHGNETAQLRRTIQALRDRLETFKNSRTAKAG"
     gene            13336..13488
                     /locus_tag="PLQ77_02520"
     CDS             13336..13488
                     /locus_tag="PLQ77_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09586.1"
                     /translation="MKNLRNQSTVKRLNARKLQTLPDVPRKVAALETLNDILDELMKI
                     TTTGWW"
     gene            13485..13763
                     /locus_tag="PLQ77_02525"
     CDS             13485..13763
                     /locus_tag="PLQ77_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09587.1"
                     /translation="MKGKTEPVNIYEVLDYHTDETYPNLMDAIHHFSDGLECYQKGQW
                     DRAMEAFRRGLSQNGGDQLSRMYIDRCEYMKNHPPSGEWNGVFVMTSK"
     gene            complement(13787..14164)
                     /locus_tag="PLQ77_02530"
     CDS             complement(13787..14164)
                     /locus_tag="PLQ77_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPL09588.1"
                     /translation="MSLTISVETKRPGFWFVSLIGRLDQTTAGECDEKITSILLPETR
                     AIVFDMTGLNYISSMGLRIILKTRRFMSGQGGAVYMMNVQPQIEKVFEIANLLKGMKL
                     FAGTKEADEYFDAIQKSVLESST"
     gene            complement(14208..15281)
                     /gene="hisC"
                     /locus_tag="PLQ77_02535"
     CDS             complement(14208..15281)
                     /gene="hisC"
                     /locus_tag="PLQ77_02535"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPL09589.1"
                     /translation="MSRFWSQTAKSIKPYVPGEQPKDRKYIKLNTNENPYPPSPKVLD
                     AIRRAADETLRLYPDPSADALRDAVASSFGIERENVFIGNGSDELLAFCFPAFFEPAG
                     LPILFADVTYSFYPVYAQLFHIPCRLIPLDEEFRLPVEGYLQENGGILIANPNAPTGM
                     GVPLETIERILRHNENSVVVLDEAYVDFGGESSVGLISRFPNLLVIRTLSKSCSLAGL
                     RVGFALGGKELIEGIIRIKDSINSYTIDRLAQAGAREAIGDAAYFEETKSKIVRTRGR
                     VAQALVNFGFRVLPSQANFLFIGHPQQPARVLFQKLREQGILVRFFDKPRIDRFLRVS
                     IGTDGEMDLFLEVVARICEKPAE"
     gene            complement(15352..16263)
                     /locus_tag="PLQ77_02540"
     CDS             complement(15352..16263)
                     /locus_tag="PLQ77_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPL09590.1"
                     /translation="MTFKGIITVIRIPFLILALTLGVLGAAVAWYESRLFGSPFYFGY
                     ALLATFGLLVAHAGVNIFNDYFDARSGLDYKTQRTPFSGGSGAAPTGQLTVSRILWLG
                     IACFAILIPIAWFFVVKSGWMLLPLLAAAAALIVFYTPFILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGAYSMSAFIASMPSFFLVHNLLLINEFPDVEADITVGRRTLPIVAGKKN
                     AAYFFSFFTVLVYCWVIGAVLFGSMPAFTLLSVLTFPIAVRVIKRALRYDDRETLLKA
                     MSANVLLVLLTQALIAAGYILSGIFKI"
     gene            complement(16356..16973)
                     /locus_tag="PLQ77_02545"
     CDS             complement(16356..16973)
                     /locus_tag="PLQ77_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPL09591.1"
                     /translation="MCGRFLLITDLSVVAQSFGAADASLPFLPERNIHPGRHIPAVIR
                     TAGGNRLASFLWGLVPFWAKDPSIGARLINARAETIAEKPSFQNAFRKRRCLIPADGF
                     YEWKKDGTPKKLFQFGLKSKEPFVFAGLYERWAPAGQKPLDTCTIITTRANEVVAPVH
                     DRMPVIVPKAGQDLWLNPEIQNPALLAEVLKPYPAREMTGEPATF"
     gene            complement(16992..18143)
                     /locus_tag="PLQ77_02550"
     CDS             complement(16992..18143)
                     /locus_tag="PLQ77_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPL09592.1"
                     /translation="MKAEDVKKIAMIGAGDMGHGIAASCLLGGYTVVLRDIDQKFVDK
                     GVAGIIKSLEKFKEKGKITPDAFAHALVRLIPMVDLKEAVKDADFIIEAVPEKLEIKK
                     SVFAEIDQYAPKHAILASNTSNISITEIASATQRPDKVIGYHFFNPAILMKLVEVIKG
                     RGTSDESVQVGYDIAKKIKKVPVIVKKDSPGFIYNRINEPTLVLLSKILEAGHPTPEE
                     FDAAFKPLMAMAPFELVDYVGIDVAAHGLEYFAQVLSPDYKPSEAMMSYLKINHLGKK
                     TGRGFYDWSAGRPAIDLAKATKEYDINQLIALQVNEATKLLEEGVADDPKEIDLAMAN
                     GGGSPFGPFALAQGIGYPELVFKLGEIYKKFPLDIFKPTNTLKEGKIKL"
     gene            complement(18209..19879)
                     /locus_tag="PLQ77_02555"
     CDS             complement(18209..19879)
                     /locus_tag="PLQ77_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPL09593.1"
                     /translation="MAERPWQKFYDYYVPQTIRYPQFPAQRIFQLTAGVQPNKVCTSF
                     YGTELTFWQVREQVLRLANALAAQGVKKGDRVGIHLPNCPQFIVAYLAVMHLGAIVVN
                     MNPLYTVNELQFIVENTSMETMITFDMFLDNVRKTAAATGLRRVIVTKITDYINGLGV
                     STAKSLDLEEGWLHFSELIENCKDTRPPRVPIVPSDPAMIQFTGGTTGFPKGALLTHG
                     NLVAATFQAGLWASPGSYSPHSMPHQERSALCVLPFFHVYGNVVAMNWSFFGCATQVI
                     VPRFDIDEILDTIVKAGHITYFPAVPTMITAIVNHPKAANLDLGRYIGLLNSGGAPMP
                     VELIERVIDLGITFNEGYGLSETSSITIANPLAYNKAGSIGIPMADNDFRLVDVENGV
                     EDVKPGEPGELLVKGPTVMKEYWNNPEETRNQLRDGWLHTGDIAQVDEDGFIFIVDRK
                     KDMIIAGGFNIYPREIEEVLYEHPKVAEAVAVGIPHDYRGETVKIFVVLKSGEKSSEE
                     EIIDYCKQKMTAYKVPKIVEFREAVPKSAVGKILRKVLRDEELAKLKK"
     gene            complement(19989..21863)
                     /locus_tag="PLQ77_02560"
     CDS             complement(19989..21863)
                     /locus_tag="PLQ77_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL09594.1"
                     /translation="MSLNQLVDSRDVRFTLFEMLELEKLNRFDRLKEFDRPVYEDTLE
                     LAEKIALQQFYTSNRDGDTVGLKFDPKTNSVKVPESFHKGFNAYIEAGFHSLAFPQEI
                     GGMGMPSSISMAAAEYFNAGNTALTMYCGAITGAIHIIQSFGSEEVKNTFLPKMFEGK
                     WGGTMCLTEPGAGSDLGALKTKAIRQPDGTFLISGQKIFISNGEHDMTENIIHPVLAR
                     IEGDPEGTKGISIFVVPKFLVNPDLTLGERNDVYCTGIEHKMGLKGNATSTLNFGDNG
                     KCIGYLLGKECEGMKVMFMLMNAARIHTGVQAEAVSSAAYMHAITYARNRVQGVHITQ
                     SMNPKAPRVAIIEHPDVKRMLLYMKSTIEGMRMLCLFLAYHEDIMHYSQSPEEVKTAT
                     GIVEILTPIVKAGISDAAWLVTAEAMQVYGGYGYCQEYPVEQYARDVKVFSIYEGTNA
                     IQSLDLQMRKILMNKDMYNYQMLKKYFADTVARAKGIVDERYVAPVERGIAKFDEIIS
                     MMVKQLQEGKFLALVANATPMQQAMFPLIVAWLHLWSLTITLPKSRELLGDAKGADRK
                     KILETNPEAAYYNGRVLSAQFFIGAEFPKFFGRVECVMNNEGAAIKVESENFTGALKE
                     "
     gene            21978..23324
                     /gene="rsmB"
                     /locus_tag="PLQ77_02565"
     CDS             21978..23324
                     /gene="rsmB"
                     /locus_tag="PLQ77_02565"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPL09595.1"
                     /translation="MKKTIRHQAVEILTRVSKSGAFAADLLDSRLEKQSLSQTADGRL
                     LTHLVYGVLRMRGRLDWILTRLYHGNYSKMEENIKNILRTGLYQLQFSDRLPAFAVVD
                     EAVKIARLFHPQAGGLVNAVLRSYLRSPDSWSFPDRDRNPAQFIAAFHSHPLWLVEEW
                     LNLFGREETQALCAADNEMPPLTVRVNLLKSGRRELMEKLIQAGFQVEETPISPDGIR
                     VSFSPSPIQKTAFFAEGLLRLQDEASQLIAYLVNPQKGQKILDVCAGSGGKATHLAAL
                     MKNEGRIVALDRNPEKIEQLRNNASKMGIGIVEAARSDGEIPLSSEYHNQFDAVFVDA
                     PCSGTGTLRRNPEIKWRLHAGDIERYAGAQKSILRNAAAAVSRNGSLVYVTCSLLSRE
                     NDEVVRQFLTDCPHYCLCPPNSHVVQNLMDPHGILRTYPHRTSMDGFFGAVFRRRI"
     gene            23386..24015
                     /locus_tag="PLQ77_02570"
     CDS             23386..24015
                     /locus_tag="PLQ77_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09596.1"
                     /translation="MFIQQMQVGHMAVFAYLVGDPVTGDALVIDPADNAHEILAEASK
                     NKLRINYIVNTHGHVDHIGGNAQMKKLTGAKIIIHEDDAFMLTSTPASMLRMFGAAQS
                     PPADLLVSDGQIISTGNIELKVLHTPGHSPGGMCLYAPGCVFTGDTLFVEAVGRTDLP
                     GGSWQTMYQAIKTKLFTLPDDTKVLPGHNYGRTPTSTIGHEKLHNPSVR"
     gene            24032..24913
                     /locus_tag="PLQ77_02575"
     CDS             24032..24913
                     /locus_tag="PLQ77_02575"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPL09597.1"
                     /translation="MHKTIAQTHFPKLKFVKRGKVRDIYAVKDYLLLVATDRISAFDV
                     IMPDPIPGKGELLNKMSLFWFKKMEDIIGNHVVSADAADFPEECAPYAEILQGRSMLV
                     EKATPLPVECIVRGYLSGSGWQEYRRDRSVSGIQLPAGLKESCKLPQPIFTPTTKAPQ
                     GEHDSPITRSEMEDIISAEITERIIQKALAIYERAAAIALAAGIIIADTKMEFGLIGK
                     KLILIDELLTPDSSRFWPADDYEEGRPQKSFDKQFLRDYLLSIVWNQKPPAPELPPDI
                     IGKTRAKYEEAYSRLVK"
     gene            25001..25399
                     /locus_tag="PLQ77_02580"
     CDS             25001..25399
                     /locus_tag="PLQ77_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09598.1"
                     /translation="MAGKKLPDYRLKQKILYIDKTSPESLISYGDLYWEAGAPSDALD
                     FYARASHSAGLQKIRDAALQNGDVFLFHGAARALGLELRDEDWENIARTAAALQKFSF
                     SVQASKKISNAERTDALAAWIKAEEAKQSA"
     gene            25396..26460
                     /gene="dnaJ"
                     /locus_tag="PLQ77_02585"
     CDS             25396..26460
                     /gene="dnaJ"
                     /locus_tag="PLQ77_02585"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPL09599.1"
                     /translation="MTKRDYYEILGVGKNATDEELKKCYRKIAMQCHPDRNPGDKQAE
                     ERFKEAAEAYEVLSDRQKRETYDQFGHAGLSNSGFQGFSGFDDVFSGFGDIFEDVFGY
                     GRGRGGRRTRPAARAGADLRYDLKISFLDAAFGITTAIELEKLHTCHECEGTGAAAGT
                     TPSTCPTCHGRGQVVQSSGFFTISSTCPHCNGHGRIIAKPCHACRGTGQEKRRKTVEL
                     KIPAGVETGSRLRLRGEGEAGELGGPSGDLYVFLHVEDHEFFLRSEDDISCRIPISIV
                     QAALGATIEVPTLKDTEKIKIPRGTQTGQTFRLKGKGIPHLQGYGRGDQIIEVFVRIP
                     TELTRKQEDLLREFEKSSRQ"
     gene            26525..26926
                     /locus_tag="PLQ77_02590"
     CDS             26525..26926
                     /locus_tag="PLQ77_02590"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPL09600.1"
                     /translation="MKSKIGRLVVCMFALMSLAGCGALVAGGAVVGAGAGTYLYANGE
                     LKTDYAASFDTVWSACEKTVAEMRGIQVQPTREIAKGTISAVINDENVKIDIAYRAKE
                     QTTVGVRVGLIGNKLSSQVIHDKIADQLAKR"
     gene            26967..27953
                     /locus_tag="PLQ77_02595"
     CDS             26967..27953
                     /locus_tag="PLQ77_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPL09601.1"
                     /translation="MNKKFLFGILLGVVLIYFSIRGIHFNDTLASLKKIHLGWAALSL
                     FFIVFMQALRSYRWGVILEPMEKISQGTLFAVTSVGFLAIAAIPARIGELARPYLISK
                     KSGVKMSSALGTIVVERVLDSLAVLTITIAVLIAQHDLPRWMFTSGILFFLLTLMMIV
                     FIVGLVWRRETAVSVIDWILRRLPGALAQKVNPLIHHFIDGFQVITDVKRLLSLLALS
                     AVVWLVDVAAIYTLLMAFGLNLPVLAAFVVMVILIAGIAIPTAPGFIGNWHYACILGL
                     SLFGIAKPQGLSFALVYHFLSMMVVVVLGAAFLPFNKFSLSEMTKQMKKTED"
     gene            complement(28102..29469)
                     /locus_tag="PLQ77_02600"
     CDS             complement(28102..29469)
                     /locus_tag="PLQ77_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL09602.1"
                     /translation="MNETILVVDDEVSICQSLKAILEDEGYQVLVAGSGEEALRVVAD
                     EMPQLVLLDIWLPGMDGLDTLKAIRAAHPNMLIIMMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKVMILVNNALNLVRLEEENILLKQKVSHQYELTGTSPVIVELKEMIGIVAPT
                     NAWILIMGENGTGKELVARSIHHLSRRSHKPIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGNKLIDMDVRV
                     LAATNKDLEKEMEAGRFRQDLYYRLHVIPLRVPPLRDRKEDIIHLTERFLLDFAAKEG
                     QPLKTLTAAALEKLMVHDWPGNVRELKNIIERLIILTPSNEIRAEDIPELSVKADMDA
                     PSFEETAAAGDSLRDARLDFERQFILKKLEEYDGNISKTAEAIGLERSNLHKKLKALK
                     VPVKE"
     gene            complement(29500..31722)
                     /locus_tag="PLQ77_02605"
     CDS             complement(29500..31722)
                     /locus_tag="PLQ77_02605"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09603.1"
                     /translation="MRLKKDARKIYLDERELRKRRRERIIMLVVGCLAIVFTILASQL
                     SAQKELPISANILVYGLTSINIILILLLLFLIVRNVFKLFSERRKGLIGSKLRTKLVA
                     AFVGLSLVPTILLFLFAINFLSYSIEFWFNIKIGDALARSLEVAQVYYTQAEDLAKFN
                     ARQVSAEITRNRLYEVDRAGYLEHFLSQRQKNYKVARLEAYFDFNDQSLVFAEGEEAA
                     STAITLSPKMLEDIYSGKEISMVTAARGGEAVVGVAPVFSYAVATEVIGRVYVSNSVP
                     QGLVDKLRSIANASEQYGQIKLLKNPIKFNYIVTLSIVTLAIIFLATWFGLSLAKSIT
                     NPIQDLVTATNRITRGDLTSRIDIEADDEVGLLVKSFNSMTDDLHKSKMGLIEANTSL
                     EERRKYMAAILRNVSAGIISIDRNGTITTINRAAESMFGIDASPYLFRNYRELLHEDH
                     LALVDSFLQEMKETRMRVLEKHLELTLRDKVLTILLTLTTIEDEEGKDSGIVVVFEDL
                     TKLQMAERAAAWREVARRMAHEIKNPLTPVQLSAQRLQRKYGDKLGEEGSVFKECTQT
                     IIDQVDVLKNLVNEFSRYARMPVTTLAPNDLNAVVSDAVALFVDAHKDIHFEFRPAEG
                     LPRFHLDAEQINRVMVNLLDNAVASLNKQTGHVGVLIRYDEAHRKAVVAVADDGVGVP
                     ADYRRKVFDPYFSTKKSGTGLGLAIVSSIISDHKGTVTVSDNHPTGTIVSFQLPVNEV
                     "
     gene            complement(31757..32341)
                     /locus_tag="PLQ77_02610"
     CDS             complement(31757..32341)
                     /locus_tag="PLQ77_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HPL09604.1"
                     /translation="MKRHKNHFWTAFITGCCMMAMMAAAPAGSAQMDPRLTDILVTGG
                     AGEMLLYARLADGFKKEMEDAILAGVPAVFTIELDVYEERPYLWDRHILRREIRRTIK
                     YDNLKKNFLIATNGLEQPAILPNFESAQKSMAELNGIPVVSLSNLTRGERYYAQVRVK
                     IDRVRLPFKMEYVLFFVSLWDFETPLYKIRFAYE"
     gene            complement(32446..>32828)
                     /locus_tag="PLQ77_02615"
     CDS             complement(32446..>32828)
                     /locus_tag="PLQ77_02615"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPL09605.1"
                     /translation="CCARTFGFLKEVEFLQARGLGLGGSLDNAIILDDDRVINKEGLR
                     MPDEFVKHKILDAVGDLFLLGMPIIGHFVAHRSGHRLNNLLLRELMRQESCWEIISED
                     HPPQSKEFKSCNIPSFTILDAVQI"
BASE COUNT         7330 a         9236 c         8947 g         7315 t
ORIGIN      
        1 gggatgaatg ggaattcaat ttcaaggccg acgcctttta cttccagtcg ctcagaatgc
       61 caaccgcgga ctggccggaa acacaggaag acacggaagg aagccttctg gagcgaatct
      121 accgaattga aaacgcggtc aaaaccatcg accagctcta tgaatccttt ctggcgattc
      181 gcttctcccc ggagtgggcg aaaaacgaaa aaccccgtct ggcgacttgg ttgaggaagg
      241 aatagctcat cactcattgc tgtctgtaaa aattacttta caaataagat taaatcgtta
      301 gaataggagc ccgtttcaat ccttggaaca gacaaaacaa agaggaggtg aagcgcttat
      361 ggatcaaaaa cagatcttca aacaaatgat ggatttcaac aagcaggcgt ttgacaatag
      421 tttcagcgtg atgtcggagc tgcaggaaca aacggaaaaa ctgatgttga attttcttga
      481 aaaggcggaa tggattcccg aagacggcaa aaaagccatg caggactgga tcgtaaacaa
      541 caagaaaagc cggaatgatt tcaaggcagc cgcggacgac agctacagaa aggtcgccga
      601 attattcgaa caggcggaaa aacagcagac accgaaaaag aaaaaataaa cctttttttc
      661 agcaaggagg tggccatgga cccgaagcaa gttgccaaac agatgattgt tttcaacaag
      721 acggcttttg acaacaattt ccgagccatg caggcgctgc aggaacagac ggaaagactg
      781 gtcggcagat tctgggaaaa atcaccgatg tttccggaag aaggaaaaaa ggcgatctcc
      841 gactggatgt ccgcctataa aaaaggctgc gacgatttca aaagcctcgt ggacgacaac
      901 tttaaaaaag tggaagactt tttcaaagaa accaaatagt ccacctgcgg cgggtctgtt
      961 ttttcacgag gtattcatgg atcaccccca cattgtcgaa cggatgaccc gtcccgattt
     1021 ttatccgcac cggccccaaa aggtcgaagc ggtgcagacg cacatttcgt atgtcttcat
     1081 cgccggcgac tacgtgtata aagttaaaaa accggtcaat ttcgggttcc tcgacttcac
     1141 gacgctcgag aagcgaaaat tctactgcga agaagagctg cggctgaaca ggcgcctggc
     1201 gccgagcatt tacctggacg tcgcgcccat cgggcgggac gacgcggggc ggctgacgct
     1261 cggcaaaacc gcgaacatcg tggaatacgc cgttttgatg aacaagctgc ccctggataa
     1321 aatgctaaaa atcctgctgg cccggggcaa agccgacgcc ggcatcatgg atgccgtggc
     1381 aaaaaaaatc gcccggtttc atcaggaagc ccagacgggc ggccgcatcg acgagatggg
     1441 ctccatcggc gtgatccgcc acaaccacga agaaaacttc gcgcaaaccg aaaaatacat
     1501 cgacgtcacc ctgtccgcct ttcaacacgg ctttctgaaa tcctatgcgg aaaaatttct
     1561 cgccgccagc gaagctctgc tggccaaacg cgtcgcggag cacaaaatcc gggactgcca
     1621 cggcgatttg cacctggagc acatctgcgt ggctgatgaa atcatcgttt ttgactgcat
     1681 tgaattcaac gaacgattcc ggttcgccga cgtggcggcc gaggtcgcct ttctgaacat
     1741 ggacctggat tacaacggtt atttcagcca gtccgcggac ttcacgaact cgtatctgaa
     1801 atactcccac gacgaagacc tgcgcgccct gttgaatttt taccgctgct attacgcctt
     1861 cgtccggggc aaagtcacca gcttccgcct ggatcagaaa gagctgccgc aagccgaacg
     1921 cgaggagatc cgccgcatcg cctcaaaata ctttgacctc gcctgcgcat acgcgtcgcg
     1981 gctggaaaaa ccggccttga ttctgaccgc cggactgatc ggctccggca agagctacca
     2041 ggcgcgcaat ctggcggatc gattcggcac ggaggtgatc cggtcggacg ttctgcgcaa
     2101 ggagctttta cggattgccc ccaccgaaaa gcatcatgaa gctttcggcc agggcatcta
     2161 ttcaggggac attacccgga agacctacga aaaagccgtt gaactggccg ccgaaaagat
     2221 cgggcggggg attccggtga ttctcgacgc ctcctttcga tgccgcgcag accggacgat
     2281 ggccgcggac atggcgcgaa ggctcggagt cccgttttat gtgattgaat gcacctgccc
     2341 cgacgacgta gtgaaaaccc gtctggaaaa aagaatgcag gaccgggaca atccttccga
     2401 cggccgctgg gaaatccttg aggagcagaa aaaacaatac gaagccgtca cggaaattcc
     2461 ccggagctgt ctctttcaca tcgacacgtc ggacaacccg gaaaatgtgc gccggcaaat
     2521 tgtttcctcg atcaaaatgg cggaacttta aggcctgtcg cccaaatatc cttttcgctt
     2581 gggcaacaac cgactgaccg tgtgatctcc ggacaggcag gtgcggcggg aacggcttgt
     2641 ttaaaacgac tgctttgcgc gtgcggtgat tcatcacgcg aaaaaaaggc gggctcagga
     2701 gtttgtcggt tctttctctt tgattttgtt cttttctttt tcttctttct tggcggcctt
     2761 ggccttctct ttgtcttcct tcaacttctc ttcccgctct tctttggcga ggcgatcccg
     2821 ggtctccgca atgtaggcct ccactttcag gtccagcccc acgccggcgt aatcgcgggc
     2881 tatgccctca aaacgcttca gagcggcatt gtattttttg tttttgaaat aaaagttgcc
     2941 gacgtaaaac tcgtgctcgg cgagtttcaa cttgcactcc cggaccatct tttcggccag
     3001 aatggaaaat ttgctctgcg gatagcgggc gatcagccgc tcgaactccc ggcgggcctt
     3061 gatggtttcc gtctggtcgc ggtcaatcgc ttcaatctgc acaaagtggc acatcccggt
     3121 ctggtaaagc gcgtagggaa tgttttcgtt ggttgggtac agagacacaa aatcacggta
     3181 ggccagctcc gcctcaccgt attccttgac gctgtaaaaa gaatccgcga tgcccagttc
     3241 ggccataatc gccagattgt gcagcggata ttcctctttg agtcgggtaa agtattccct
     3301 ggcctttttg taggagccgt tctggtaatc cgccgtgccc cgggaataga ggccttcggg
     3361 ggttgaccgg accggatcgc tcttcacaaa gaacgacttg atggctgcca tgttgaaaga
     3421 gcagccggta cagatcagca ccatggccag aatgacgctt gcgtgtctta acttcacagg
     3481 gatacccgtt ccatcaggaa tgaaattttt gcttccgttt gctttgcgta atgccttcac
     3541 aggcttttct tcacaaactc ttcgagcttt tccttcggcg tcatgccgac cagcgtgtcc
     3601 agcactttcc cgtctttaaa caggatgaga gtcggaatgc tgcggacgcc gaaggcgacg
     3661 gccgacttct ggctttcatc cacattcagt ttcatgacct ttacccggtc cgaatattcg
     3721 ccggcgattt cttccacaat aggaccgatg gccttgcacg gtccgcacca gggcgcccag
     3781 aaatctatca gaaccggctt gtcgcttttc aggacttcct gctcgaaatt tgcatccgtc
     3841 gccacaccca gaaattggcc gctcatgatt cgcaacctcc ttttctattt gggagttata
     3901 tgtcacagcg tgtttcaaaa agtcaaatgc tatttgaccg cctttcccgc aggcgtgatt
     3961 cttttgacgc ccgccggttt ttctgctatg aaatggagca catcgggagg ttagtcatga
     4021 aacgtttggg cgtcttatca gggacattca tgctggagaa aagcgactgg atccggaaag
     4081 gcaaaaggca acggatccac aacgtttacg gagaggcggc ggcgatcgaa acggattcca
     4141 tcgtcttcat tctcaggcac ggcgacgatc ccaacgacta tattctgccc caccggatca
     4201 accaccacgc caatctccag gcgctgcaag accttaacgt caccgaagtg atcggcatca
     4261 actctaccgg gtccctgaaa acaaaacttc gtccggggat gattgtcatt cccgacgatt
     4321 tcatcacctt tgcggcaacg ccgtccattt atcataacaa agccgtccat atcacaccct
     4381 ctttaaatga ggccgtacgc caaaaactga ttacagcggc acacgactgc aatctggatg
     4441 tcgtggagac ggggacttac tggcaggttg cgggcccccg cctggaaacc cgggcggaga
     4501 tccggatgat gtcccatttt gccgatgtcg tcggcatgac gatggcccat gaagcggttg
     4561 ccgcgctgga gttggggctc ccctacgcct cggcctgctc catcgacaat ttaggcaacg
     4621 gcctgctgga aaagcctttg agcctggagg aaatcatcgc cggaacacgc aaaaacgcgg
     4681 acctgatgat ccgcctgctc gaggagtata ttgagggggt cgacaaatag gctatttaca
     4741 ataatatcaa ttatttatag cttcacgggt tcaaagtttg caatgctgga actccggcgg
     4801 ctccaggatg gtgatctttc tgcgcttgag gtactccgga aaaccgtaaa gccgattgcc
     4861 gcgatactcg acgaccacgg catccgccgg acagttgttc aggcagccga agcaggcaat
     4921 acacttatca tgatccacaa actgcctctc cggatgaatc gcgtccaccg gacacatttt
     4981 gacgcaagtc cggcagccga tgcatttggc gccgtccacc gtatgtttgc cgacggcttt
     5041 tttgttgagc cagaccaaag gaagcatccg cacgatttcc cgaagcgcaa tttcggagga
     5101 ataggcaagg gattgaccct gccgcactct ttcctgaatc tgtttggcga attcccgaac
     5161 ccggttgtac gtcgcctcgt cgggcagatg ctccccggag cgctgattgg cgctgtccca
     5221 actggcagga taggccggaa tgctgcggaa ggcctccatg cccacgggca cggcttcctt
     5281 ttccgccagc agatgaagcg cgtggcagag tgcgttgtgc tgattgcctt ccggcccgcc
     5341 gaaggacaca aacgccgcca cgggaattcc cgtcagcttc ggcgcggaag acagccagtc
     5401 cgccacgttt tccggaatat cgtaatagaa aaccggagag ccgaccagaa tcaaatcgta
     5461 atccggcagg cgcttcggat cgaaacggcg catgtcggcc cattcaaccg tcaatccctg
     5521 atccttcaga agacaggcga tcagttttcc gtagcgcgcg gagaatcccg actggctgta
     5581 gctcacaacc agcgcgcgtt tcggatcgcg ggttttgaat tcccgttgga cggtctgtgc
     5641 gttcgcggcc cacggcagcg gcaggcttaa agcggcgcag accaccacgc tttttttgat
     5701 gaagtctctt ctggtttcca tggaatcggc ccggcctttc cgcggcgcct gattattctt
     5761 tcttttcttc gccgccgaaa ttgattttca actcatagac tcccatgtcc agatgcgtgg
     5821 tcatcggata gccgcacttg gagaaaacat gcatcatcgg caggttgcgc gacaaaacgt
     5881 ccgccgtgaa gccttcgata ttcttcccct tcgcgatgcg gatcaaatac tgcaggagga
     5941 aggtgccgat gccccggtcc tgccagtcct gatgcgtggt gaaggccact tcggcatagc
     6001 gggtgttggg attgttggcg tagtttccga tggccacgat cttttccctc ccctccggct
     6061 cctccttgcc gatgaccgcc acgatggcca tcttgtcgtc ataatcgatg gccgccatcg
     6121 gcatggccac tttgtgggaa aaggacttta aattgtgaaa gaaccgcgta tagacatcct
     6181 gcggcggcag atcgtagatc aattcctgga tggcgcgttc gtccgtggct tttaccggac
     6241 ggaaaaacag cgaggttccc ttcttgtcca cccaggtgat ttcatactcc ttcggataca
     6301 gcaccaccgg cagcgtctga tcccggtaaa tgtagccctg acgcttggcg gcttccagca
     6361 gctcgtcgcg gaacttcgga tgggcgatgt tgatgagcat catggcccgg tcgcggatgg
     6421 atttcccgtg cacgtaggca atgccgtatt ccgtcacgac ataatggatg tccccccgcg
     6481 tcaccactac gccgctgccc gcggagggat acggcacgat gcgggagatg gccccgttat
     6541 ccgtggtcga aggcagcacc atgatggatt tgccccgatt ggacatggcc gaaccgcgga
     6601 caaaatccac ctgcccgccg atgccgctgt aaaactcgaa tccgagggaa tccgaacaca
     6661 cctgcccggt gagatcaacg gtcagcgcgg aattgatgga caccatccgg tcgttttggg
     6721 cgatgacata cggatcgttc acgtactgac acggccggaa ttcaaagagc ggattgttgt
     6781 gcacatagtc atacaggcgt cgggtgccca tgcagaaggc cgcgacaatc tttcccggat
     6841 gcagcgactt cttccggcag gtcacgacgc cgctttcaat cagatcgatg aggccgtcgg
     6901 taaacgtttc cgtgtgcacg ccgaggtctt ttttattggc cagatagggg aaaacggaat
     6961 tgggaatctt gccgactccc gtctgaatgg tggattcatt gtcgatcaga tcggcaatca
     7021 gccgtccgat ggactgagcg atgtcatccg gcgccttctg ttcgaattcc agaagcggcg
     7081 cgtcgttttc cacaaacgcg gaaatctcgc tcacatggat aaaactgtcg cccagcgtcc
     7141 gcggcatgtt cggattcacc tccgccacca cataccgggc cacggaagcc gccgtcttgg
     7201 tgatgtccac ggaaatcccc aggctgacat agccgttcat gtccggcgga gagactgtaa
     7261 tgagggcgac atcaatgggc atggatcccc gcttcatcag caggggaact tcggaaagga
     7321 aaatgggcgt gtagtcggcg tgcccttcct tgatggccgc gcgggcgttc gcgccgatga
     7381 agagcgcgtt gtgccggaaa tgctccgtgt agatttccga cgtgtagtcg gtgagcccca
     7441 gatccagaaa atggatcacc tccacgtcgg cgaaattctg cgccatatcc agaagggttt
     7501 tgaccagaag ctgcggctcg ccgcaggcgg aagcaatgaa gatgcgcgct ccgcgccgga
     7561 tttgggacaa agcgctccta gcatcggtga gtctttcctt gtaggtatcc tgccagttga
     7621 gaatcatgtt gctcaccgct tctttcatgc atcgagtgct ttgaaaaact actcttgatc
     7681 aacctgtaaa aagtcataat ttacaaatat tgtcatggtg agctcgtcga accatgaccc
     7741 cctcccccca cccctcccac aaggggaggg gagataaaaa gacgcagaag gtaaactgtg
     7801 gagccacttc acatttactt ccctctccct tagagagaag aggagataaa agaggaggtg
     7861 gggcatcctt ttacttcttc ttcgcctttg gcccactcgc cgacgtaatg gagataaaag
     7921 aggaagtggg gcatcctttt acttccctct cccttgaggg gagagggtta gggtgagggt
     7981 gagggtgaaa aaactcccat caaaaagctc cgagctgaca gggcttgacc ttgattttca
     8041 ggttttcaca ggcggcgcag acctccatac gcttgatgcc cagccgtgcc gcgatctccc
     8101 aggccgcggc gcaggacaac cggccgttga ccagcgcttt gcggatggcg gcatccaggt
     8161 ccggtgcgac ggatggcgcg ggcagcactt tcttttgctt gccgtccagc ccgaagaggc
     8221 cgagctggca ctgcgcaata ttaatattga gcatgtcaat ggccacgccg acttcgccaa
     8281 gcgaaaaacc tgtgctctgg gcaatttttt ccgccgcctt gcaggaaaca ttgttgtctt
     8341 ttgcggactt ctgaaccgcc tctttcagct tgtcgctgac ggttgtccct tcgggatgct
     8401 tcttgaagta tttgcctttg tcctgatgtg ccacggtttt ctctcctttc ctctgagaaa
     8461 tcgaggttga aaatatcacg atcttttgca ttcgaaggat aacgcggcgg ctaggaggag
     8521 gcaccgcagg cgtattatga atacgtcgag gatgccgacg acaacgccaa caaagttagc
     8581 cgatgaatgc ggaagatcgt caatatttca tgtaatgaaa cggctccacc gccgaaagcg
     8641 cctcggtatc cagtgtcttg aatccgttct tttccacaag tttttccgtc ttttcaatct
     8701 ggtccacgtc caccacaaac acggcttttt ccgcgcattc cagaacaaag ccgcaggcat
     8761 tgttgatatt gatgccttcc ctgtacaaaa gctgcatcaa cttatccagc ccccccggcc
     8821 ggtcgggaat cagcacggcc agaacgtttt tcaggtgaac cgtcaatccc gcgttggcga
     8881 gcgcctttgc agcgcttttc ggatcatcca caatcaggtt gatcaccccg gatgtgccgg
     8941 cggtcgagat ggttgtcgcc cgaatgctga tatcggcctt tgccagaacg cttgtgacat
     9001 tggccaattg ccccggctta tcttccgcga aaacacaaag ctgatacgca atcatgttct
     9061 tctcctgatt ttctttgccg ttgagtcttt ttacaatgat gaccaagttt tagcaagtca
     9121 aatctcggat tcgaccttcc gccagcggcc gtcttccaga accgctttac ccctctgccg
     9181 caaataatcc aggtgctttc cgatcagaac cctttcgttg aattcgtaca tctccttcgg
     9241 ctcgcggggc ctgccgtaaa tcagccaggc cgagcagatc tcacggggcg ttttcggccg
     9301 gtccaggaag gccagcagct ttctttcccg ctcataaatg atcttttcat actgatccca
     9361 gagcgggccg ggattggatt cataaattcc gtgattatgc gcggccaccc atcgcctggc
     9421 cggtatcgct ttgagcttgt gaatcgaggc gatggtatgt gcgatgtcgg ataaaacatc
     9481 gccataccac ggcccgaacg acgtcagatc gtaatccccc agaaacagca gatcctcttc
     9541 cggaaaaaag aacgacaggt ggccgggcgt gtggccgggc gtgggaatga cctgcacggt
     9601 gagcccgccc agatccatcc tcttgtcatc ctgaagaaaa cacgccgttt gacgcgggcg
     9661 gtaatcaaac gtttccagca tttccttctt ccaatagtat cgggcgcccc tgtcttcaat
     9721 cccgtaccag tccagaaatg tttccagatc ggtaaacggc ggaaaatcgc gttcggagac
     9781 ccaaaccgga caaccttcaa agaggttcat gtagcgaatg tgatcttcat gccagtgcga
     9841 aagccagacg ccatcaacgc cttcctcctt caaccgcgtc aacttttcca gcgaagaggc
     9901 gggatcgatg accactcgct cccgatcgcc ctgcagatac agggaatggc taaagggata
     9961 cttcccgccg ttttcaccgc gaataaatcg aaccctgccg aattgacgat ctttcatgac
    10021 tgaaactccc ggtatggtgg tggtggaata tagaaaatat ccggccaaat tgccatcaag
    10081 aattccataa gcgaaaactc gttgaaacaa atggtttttc cagttactgt atcttgcagg
    10141 atcatttgag atcgttggtt ttccactgca acgcatcgga taatgacagg ccgcagcgga
    10201 aaagtgagcg gcggccgggg agacgggatt gaggagcatc aggacaggcc gatgatcaag
    10261 gcacttttct gggacaatga cggcattttg gtcgatacgg aaggcatttt tttcagggcc
    10321 aatcaggaga cgctggcgga tgtgggcatt tcgcttcaat gggagcagtt tgaagaaatc
    10381 tcgcttgtcc ggggcgggag tgtgctgcaa ctggcggaaa agccgcaaac ggaggcgttt
    10441 tataaactgc gcgacaggcg ggacgctctt tacgccgacc tgctgacgcg ggagccgctg
    10501 gtcatggaag gcgttcggga agtgctggag gcgttgaagg gaaaatacgt gatgggaatc
    10561 gtaacgagct ccggacagga gcacttcgag attattcaca gccggtcggg gcttctgcct
    10621 tattttcaat ttgctctgac catgaaagac tgcgcgcatt tcaaaccgca tcccgagccg
    10681 tacctcaaag cgatcgcgct ctccggctgc cagccggagg aatgccttgc cgtcgaggat
    10741 tcgccgcgcg gtcttgcctc cgctgtaaac gcagggattc ggtgcatggt ggttccgagc
    10801 cgattcacac ggggcggcga tttcacggga gccttcagaa tcctgaacaa catgagggag
    10861 cttccccaag cgctggattg atctgtcccg aatcaaccga tcattgtttt gcggagtcgt
    10921 ccatcaaccc tccgtctccg cttcgacaag gctcccggcg tagtatttct cgcggagctt
    10981 cggcttttca atttttccgg tgggattgcg cggcacctgt tcaaaaaaga ttttgcgcgg
    11041 acgcttgtag cgcgggagcg cctcgcagaa ttttgccatt tcggcttccg agccggcaaa
    11101 acccggctta agctcgatga ccgccgcgac gatctccccc agccgatggt cgggcagtcc
    11161 gatcaccgcc gcatccttga tggcttcgtg cgtgcgcaga aagtcctcga tctgcaccgg
    11221 atagatgttt tcgccgcccg tgatgatgac gtccttcttg cggtccacca gatagacaaa
    11281 gccgtcttcg tccatccgcg ccatatcgcc cgtgaacagc cagccgtcct tcaggatctc
    11341 ggccgtggct tcggggtttt tgtaatagcc tttcatcacg ccgtcaccct tgacgcaaag
    11401 ctcgcccacg tcgccctgct tcacgggagc accgttttca tccacaatcc ggatttccca
    11461 ctgatagccg ggctttccga tggcgccgac tttgtggata ttttccagcc ccaggtgaac
    11521 gcagcccgga ccgatggatt ccgacaggcc gtaattggta tcgtaatcgt gggttggaaa
    11581 atgttccctc cagcgccgga ccaggctcgg cggaaccggc tgcgcgccga tgtgcatcag
    11641 ccgccactgg tcgagctgat agtctttcag cttgaggtcg ccccgctcga cggcgtccag
    11701 aatatcctgc gcccacggca ccagaagcca gacgatcgta atcttctctt cggacaccgc
    11761 cctgaaaacc cattccggtt tgacgccgcg cagcaaaacc gcgcggcttc cggccaggaa
    11821 gcttccgaac cagtgcatct tcgcgccggt gtggtagagc ggcggaatgc acaggaaatt
    11881 atcttcgcgc gtctgccggt gatggttcat ttccacttca caggccatca tcaggctgcg
    11941 gtggtgatga aggatcgcct tgggaaagcc ggtagtgccg gatgaaaaat agatggccgc
    12001 gtcgtccgcg tcggaaagcg gaacgcgcgg cgccacggag gagcagcacg cggtgagcgc
    12061 gtagtagctt tcggcgaacg cgggcaggtc ctgtcccaca aacaggcgga gttttacttt
    12121 cggcatctgt tcgcggatcg cttcaattcg ctgggtgaat tccggcccga agatcaaaac
    12181 gtcgcaatcg gccagatcca gacagtattt gatttcttct tccgtatagc ggtagttgag
    12241 cgggacggca atggcgcccg tcttgaggat gccgaaatag atcggcagcc attccagaca
    12301 gttcatcagc aatattccaa ccttgcgccc cttttccaga cctctttgca aaagaaagtt
    12361 ggccaggcgg ttggctttgt cgtcaaactc gcgccaggtc atctcccgac ggtagcgatt
    12421 ttccggattg gtttcgacca gctcataatc cttccagctt gcctttactt ctttgatttg
    12481 aggattgatc tccaccaggc ttgtttcttc gcagtagaga gaagcgtttc gggttagaat
    12541 ttctgtaatg ggcatttagg attccttctg ctcatgggat tgatggattt gcggcggtat
    12601 tccagaatat tttgacatat cagctgcaca cggcacaaca acacgttttt ttcggaacgc
    12661 acgatacgaa tttcaggcgt tgatgtcaag aatatcttgc cggccgggaa acggcttcgc
    12721 cgcatcctca gtggaaaagg acatggtaaa tcacgtaaac ccacgagaga atgccgtgaa
    12781 agatggccca gagcaccgaa tgactgtagc tgtagctgat ggtgatggcc agcgccgtgc
    12841 cgaacgtaat tccggtggca atgatatgac cctgcatgac aacctcctgt gattgcgtag
    12901 gatttcgatg ttctcgggcg gcattataga aattcccgcc ttccctgtca agaaaacagg
    12961 gggaacaggc gttctccctc aaattatttt cggttgaaat cgcaccggaa ttgactttat
    13021 aaagcgcgcc aacgtcaaat acaaagcgcg ccgccgtttc cgccatgcgg gatcagctgg
    13081 aaaacacctg ctatgaaaaa gattgcgcgg cgcaggagga tatggccgcc gcagggaaca
    13141 gggaaaagca gttaaccaac acgatagcag ccctgcgcaa tgaactggag cgccgggaca
    13201 tcgatcgtca gcgggacatg caaacgatgg ccagatcgca tggaaatgaa acggcgcaat
    13261 tgcgccggac catccaggcg ttgagggacc gtctggagac cttcaaaaat tcgagaaccg
    13321 caaaggccgg ataaaatgaa aaaccttcgg aatcaatcaa cggtaaaacg cctgaacgcc
    13381 aggaagctgc agacgctgcc ggatgttccg cgaaaagtgg cggcgctgga aaccttaaat
    13441 gacatcctgg acgaactgat gaaaatcacc acgaccgggt ggtggtgaag ggaaaaacag
    13501 agccggtgaa catctacgaa gtgctcgatt accataccga cgaaacgtac cccaacctta
    13561 tggacgccat ccatcatttc tcggacggcc tggaatgcta tcagaagggg cagtgggaca
    13621 gggcgatgga ggcgttccgg cgcggattgt ctcaaaacgg cggcgatcag ctttcccgga
    13681 tgtatatcga ccgctgcgaa tacatgaaaa accacccgcc gtccggcgag tggaacggcg
    13741 tcttcgtcat gacgtcaaag taagcgcgca aaaaaagtct caggccttac gtcgaggatt
    13801 ccaaaacgct tttctgaatg gcgtcgaaat actcgtcggc ttccttcgtg ccggcaaaca
    13861 gcttcattcc cttcagcagg ttggcaatct caaaaacctt ctcgatttgc ggctgcacat
    13921 tcatcatgta aacggcgccg ccctggccgg acatgaatcg cctcgttttc aaaatgatgc
    13981 gcaggcccat gctggaaata taattcaggc cggtcatgtc gaagacgatg gccctggttt
    14041 cgggcagcag gatcgacgta atcttttcgt cgcattctcc ggcggttgtc tggtcaagcc
    14101 gtccgatgag cgaaacgaac cagaacccgg gccttttggt ttccacgctg atcgtgagag
    14161 acattgcctt tctccttatc gtgaaatcga aacgccgccc gtgaaaatca ttccgcgggc
    14221 ttttcgcaaa ttcgtgcgac gacttccaga aagaggtcca tttccccgtc ggttccgatg
    14281 ctcacccgaa ggaaacgatc gattctgggc ttgtcgaaaa agcgcacaag aatcccctgc
    14341 tccctcagct tttggaaaag aacgcgggcg ggttgctgcg ggtggccgat aaatagaaag
    14401 ttggcctgcg acggaagaac ccggaatccg aaatttacca gcgcctgcgc caccctgccg
    14461 cgggtccgga caatcttcga ttttgtttcc tcaaaatacg cggcgtcccc gatggcttcc
    14521 cttgcgcccg cctgggccag acggtcaatc gtgtaggagt tgatggagtc tttaatccgg
    14581 atgattccct cgatcagctc ttttccgccc agggcaaatc ccacccgcag tccggccagc
    14641 gaacaggatt tggacagcgt tcggatcacc agcagattcg gaaagcggct gatcaggccg
    14701 acggacgatt cgcctccgaa atcgacatag gcttcgtcca gcaccacgac gctgttttca
    14761 ttgtgccgca aaatcctttc gatggtttca agcggcacac ccatgccggt gggcgcgttg
    14821 ggattggcaa tgaggattcc cccgttttcc tgaagatagc cttcaaccgg caaacggaat
    14881 tcttcatcga gcggaatcaa acgacaggga atatgaaaaa gctgcgcgta cacgggatag
    14941 aagctgtagg tgacgtcggc aaaaagtatg ggaaggcccg ccggctcgaa aaaagccgga
    15001 aagcagaagg ccaggagttc atccgagccg ttgccgataa acacgttctc cctctcgatg
    15061 ccgaaggacg aggcgacggc gtctctcagc gcatcggccg acggatccgg ataaagccgc
    15121 agcgtctcgt cggccgcgcg ccggatggcg tcgagaacct tcggtgaagg cgggtacggg
    15181 ttttcgttgg tgttgagctt gatgtatttc cggtcttttg gctgttctcc cggaacataa
    15241 ggcttgatgc ttttggctgt ctggctccag aaccggctca tatccgctca tccgttggtt
    15301 gtctttttac actttgcaga tttcatatag gaaacgcgcc aaaatttcca ttcatatctt
    15361 aaagatgccg ctcaaaatgt atcccgccgc gatcagcgcc tgggtaagca ggaccagcag
    15421 aacattggcg gacatggctt taagcagcgt ttccctgtcg tcataccgca gggctcgttt
    15481 gatgacacga acggcaatgg gaaaggtcag cacggacaaa agcgtgaagg cgggcatgga
    15541 accgaagagg accgcgccaa tcacccagca ataaaccagc acggtgaaga aagagaaaaa
    15601 ataagccgca tttttctttc cggcgacgat cggcagcgtc cgcctgccca cggtgatatc
    15661 cgcttccacg tccggaaact cattgatcaa aagcaggtta tgcaccagga aaaatgaagg
    15721 catcgaggcg atgaaggcgc tcatcgaata cgcgccggtg tgaacgaaat aggcgcccat
    15781 gaccggcagg atgcccaatc ccaggccggg cgaccactcg gggtatccca tcttgaggat
    15841 gaacggggtg taaaaaacaa tcagagcggc ggccgccgca agcagcggta gcagcatcca
    15901 gcccgatttc acaacaaaga accaggcgat cggaatcagg atggcaaagc aggcaatccc
    15961 cagccacagg atccgggaaa ccgtcagttg gcccgtcggc gccgcgccgc ttccgccgct
    16021 gaaaggggtg cgctgcgttt tataatccag gccgcttcgg gcatcgaaat aatcgttgaa
    16081 aatattcacg cccgcgtggg ccaccagaag gccgaacgtg gccagaagcg cgtagccgaa
    16141 ataaaacggc gagccgaaga gccgggattc ataccaggcg acagcggctc ccagaacgcc
    16201 cagcgtgagc gccaggatca gaaaagggat gcgaataacg gtgatgatgc ctttaaatgt
    16261 catgagtttg tccgttgaca atcctttctg tgaaatcgga ctgctcttta gcagaggatg
    16321 cctcttcttg gcaaaccaat tcttcaggac atccgctaaa aagttgccgg ctcaccggtc
    16381 atttcccggg ccggatacgg tttcaatact tcggcaagaa gggcgggatt ttgaatttcc
    16441 ggattcagcc acaggtcctg cccggccttg ggcacgatca ccggcatgcg gtcgtgaacc
    16501 ggcgctacaa cctcgttggc tctcgtcgtg atgatcgtgc aggtgtcgag cggtttttga
    16561 ccagcgggcg cccaccgctc atagaggccg gcaaaaacaa acggttcttt cgatttcagt
    16621 ccgaattgga aaagtttttt cggcgtcccg tcttttttcc attcgtaaaa tccgtcggcg
    16681 ggaatcagac atcgtcgctt ccggaaggcg ttttggaagg agggtttttc cgcgatcgtc
    16741 tccgcgcggg cattgatgag cctcgcgccg atggacgggt ctttcgccca gaagggaacc
    16801 agcccccaca aaaaagaagc cagcctattc ccgccggcgg tacggatcac cgcgggaata
    16861 tgccggcccg gatggatgtt gcgctcaggc aaaaacggaa gggaagcatc ggccgcgcca
    16921 aagctttgcg cgacaaccga taaatccgtg atcaacaaaa accttccgca catgggcttt
    16981 ttagatttgc ctcacagttt gatcttgccc tctttcaggg tattcgtggg tttgaagatg
    17041 tccaggggga attttttgta aatttcaccc agcttgaaca ccagctccgg gtacccgatg
    17101 ccctgggcca gcgcaaaggg gccaaagggc gagccgccgc cgttggccat cgccagatcg
    17161 atctccttgg ggtcgtccgc aacgccctct tccagaagct tcgtggcttc gttgacctgc
    17221 agcgcgatga gctggttgat gtcgtattcc ttggtcgcct tcgccagatc gatggcgggc
    17281 ctgccggccg accagtcata aaatcccctg ccggttttct tgcccagatg gttgattttc
    17341 aggtaggaca tcatcgcctc ggagggcttg tagtccgggg acagaacctg cgcgaaatac
    17401 tccagtccgt gcgcggccac gtcgatgccg acatagtcca ccagttcaaa cggcgccatc
    17461 gccatcaggg gcttgaacgc ggcgtcgaac tcttccggcg tcggatggcc cgcttccagg
    17521 attttggaca gcagaaccag cgtcggttcg ttgatccggt tgtagatgaa gcccggggaa
    17581 tccttcttga cgatgacggg aacctttttg atctttttgg cgatgtcata gcccacctgt
    17641 acggattcat cggacgtgcc cctgcccttg atcacttcca ccagcttcat caaaatggcc
    17701 ggattgaaaa aatgatagcc gatgaccttg tcgggccgct gcgtggcgga ggcaatctcg
    17761 gtgatgctga tgttggaagt attggaggcc agaatggcgt gcttcggcgc gtactggtcg
    17821 atttcggcaa aaacgctctt tttgatttcc agcttttcgg gcacggcctc aataatgaaa
    17881 tccgcgtcct ttacggcttc cttcaggtcc accatcggga tcagccgcac aagcgcgtgg
    17941 gcaaaggcat ccggtgtgat cttgcccttc tctttgaatt tttccaggct cttgatgatc
    18001 ccggcgacgc ctttatcgac gaacttctga tcgatgtcgc gaagaacgac cgtatatccg
    18061 cccagcaggc aggacgccgc gattccgtgc cccatatccc ccgcgccgat catggcaatc
    18121 ttttttacat cttcagcttt catgttgcat ctccttttct gaaatgaata atcaggcggg
    18181 aaacggtttc gtttcccgcg attgtgcctc attttttcaa cttggccagc tcttcatccc
    18241 gcaggacctt gcgcagaatc ttgccgacgg ccgacttggg aacggcctca cggaattcca
    18301 cgatcttggg caccttgtag gccgtcatct tctgtttgca gtaatcgatg atctcctcct
    18361 cgctggattt ttcaccggac ttcaggacga caaatatctt caccgtctcg ccgcggtaat
    18421 catgcggaat gccgacggcc accgcttcgg ccaccttggg atgctcgtag aggacctcct
    18481 cgatctcccg cggataaata ttgaagccgc cggcgatgat catgtctttc ttgcggtcca
    18541 cgataaagat gaagccgtct tcatccacct gcgcgatgtc gccggtgtgc agccagccgt
    18601 cgcgaagctg gtttctggtt tcctccggat tgttccagta ttccttcatg acggtcggcc
    18661 ccttgacgag cagttcgcca ggctcgcccg gtttgacgtc ttccacgccg ttttccacgt
    18721 ccaccaggcg gaaatcattg tccgccatgg gaatgccgat ggagccggcc ttgttgtagg
    18781 caagcggatt ggcaatcgta atggagctgg tttcgctcag gccgtatccc tcgttgaagg
    18841 tgatgcccag gtcgattacg cgttcgatca gctccacggg catcggcgcg ccgccggaat
    18901 tcagcaggcc gatgtaccgg cccagatcaa gattggccgc cttgggatgg ttgacgatcg
    18961 cggtgatcat ggtgggaacc gccgggaaat aggtgatatg cccggccttg acaatcgtgt
    19021 ccagaatttc atcgatgtca aagcgcggaa cgatcacctg tgtggcgcac ccgaaaaacg
    19081 accagttcat ggccacgaca ttgccgtaaa catggaaaaa gggaagaacg cacagagcgc
    19141 tgcgttcctg atgcggcatc gagtggggcg agtagctccc gggcgacgcc cacagaccgg
    19201 cctgaaaggt ggcggccacc aggttgccgt gcgtcaaaag cgcgcccttg ggaaagcccg
    19261 tcgtgccgcc ggtaaactga atcatcgccg gatccgaagg cacaatcggc acgcggggcg
    19321 gacgcgtgtc cttgcagttt tcgatcagtt cggaaaaatg cagccagcct tcctccagat
    19381 cgaggctttt ggcggtgctc acgcccagcc cgttgatgta gtccgtgatc ttcgtcacga
    19441 tgacgcgcct gaggcccgtt gccgcagccg tcttgcggac gttgtccaga aacatatcaa
    19501 acgtgatcat ggtttccatc gaggtgttct ccacgatgaa ctggagttcg ttcaccgtgt
    19561 aaagcggatt catgttcaca acgattgcgc ccagatgcat gacggccagg taggcaacga
    19621 taaactgcgg gcagttgggc agatgaatgc ccacgcggtc gccttttttc acgccctgcg
    19681 cggccagcgc gttggccagc cggagcacct gctcgcggac ctgccagaag gtcagctcgg
    19741 ttccgtagaa actggtgcag accttgttcg gctgaacgcc ggcggtcagc tgaaagatcc
    19801 tctgcgccgg aaactgggga tagcggattg tctgagggac atagtagtca taaaatttct
    19861 gccagggtct ctctgccatc ttgtaaacct cctttgccag tcgtgatcag acgcgcggag
    19921 cggtcccgcc ggtcccctcc ccgcgacggt tgttgtcagc ctctttattc ccggtgtttc
    19981 aggcgtcgct actccttcag cgcgccggtg aagttttcgg attcgacctt gatcgccgcg
    20041 ccctcgttgt tcatcacgca ctccacccgg ccgaagaatt ttggaaattc cgcgccgata
    20101 aaaaactgcg ccgagagcac ccggccgttg tagtaggcgg cttcgggatt ggtttccaga
    20161 atctttttgc ggtccgcgcc tttggcgtcg cccagaagct ccctggactt cggaagggta
    20221 atcgtcaggc tccacaggtg cagccaagcc acgatcagcg ggaacatcgc ctgctgcatc
    20281 ggcgtggcgt tggccaccag cgccaggaac ttgccttcct gaagctgctt caccatcatg
    20341 ctgatgattt catcgaattt cgcgatgccg cgctccaccg gggcgacgta ccgctcgtcc
    20401 acgatgccct tggccctggc aacggtgtcc gcgaaatatt tcttcagcat ctgataattg
    20461 tacatatcct tgttcatcag gatcttgcgc atctgcagat ccagcgactg aatcgcgttg
    20521 gtgccttcgt agatcgagaa aaccttgacg tcgcgggcat actgctcgac cgggtattcc
    20581 tggcagtagc cgtagccgcc gtaaacctgc atggcttccg ccgtcaccag ccaggcggcg
    20641 tccgaaatgc ctgctttcac gatgggggtc aaaatttcaa cgatgcccgt cgccgtcttc
    20701 acttcttcgg gcgactggga gtaatgcatg atgtcttcat gataggcaag gaagaggcag
    20761 agcatgcgca tgccctcgat cgtgctcttc atatacagca gcattctttt cacgtccggg
    20821 tgctcgatga tcgcaacccg gggcgccttg ggattcatgg actgcgtgat atggacgccc
    20881 tggacgcggt tgcgggcgta tgtaatggcg tgcatgtaag cggctgagga cacggcctcg
    20941 gcctgaacgc cggtgtggat gcgggccgcg ttcatgagca tgaacatgac cttcatccct
    21001 tcgcattcct tgcccagaag gtagccgatg cacttgccgt tgtcgccgaa gttgagcgtc
    21061 gaggtggcgt tgcccttgag ccccatcttg tgttcgatgc cggtgcagta aacatcgttt
    21121 cgctcgccaa gcgtcaggtc ggggttgacc aggaacttgg gcacgacgaa gatggaaatg
    21181 ccctttgtcc cttccgggtc gccttcgatg cgcgccagca cgggatggat gatgttttcc
    21241 gtcatgtcat gctcgccgtt ggagatgaag attttctggc cggaaatgag aaacgtgccg
    21301 tcgggctgcc gaatggcctt ggtcttgagc gctcccaggt cggagcccgc gccgggttcg
    21361 gtcaggcaca tcgtgccgcc ccatttgcct tcgaacattt tgggaagaaa agtgtttttc
    21421 acttcttcgg aaccgaacga ctgaataatg tggatggcgc ccgtaatggc gccgcagtac
    21481 atggtcagcg ccgtgttgcc cgcgttgaaa tactcggccg ccgccatcga aatcgacgag
    21541 ggcattccca ttccgccgat ttcctgcgga aacgccagcg agtgaaaacc cgcctcgata
    21601 taggcgttga atcccttgtg gaaggattcc ggaaccttga cggaattggt cttgggatca
    21661 aacttcaatc cgactgtgtc gccgtcgcgg tttgacgtat aaaactgctg cagggcgatt
    21721 ttttcggcca gttccagcgt atcctcgtac accgggcgat cgaattcttt cagacgatca
    21781 aaccggttga gtttttccag ctccagcatt tcaaacaggg taaaacggac atcacgggaa
    21841 tcgactaact gatttaacga catacctgcc tcctttgata attttacaac gagtgtttgt
    21901 tgtttttctt aacagaacaa ttgcgaaaag caagaaatat attgtgaatc aaaccgggtt
    21961 ttactatacg agacaaaatg aagaaaacca ttcgacatca ggccgtggaa atcctgaccc
    22021 gcgtttcaaa aagcggcgct ttcgcagccg atctgctgga ttcccgcctt gaaaaacaaa
    22081 gcctctccca aacggcggat ggccgtctgc tcactcatct tgtctatggc gttttgagaa
    22141 tgcgggggcg cctggactgg attctgaccc gtctgtatca cggcaactac tcgaaaatgg
    22201 aagagaatat taaaaacatc ctgcgcacgg ggctgtatca gcttcaattc agcgaccggc
    22261 tgccggcctt tgcggtcgtg gatgaagcgg taaaaattgc ccggctcttt catcctcagg
    22321 cgggcggact ggtcaacgcc gtcctgcgaa gctatctgcg cagcccggac tcctggtcct
    22381 tccccgacag agaccggaac ccggcccaat ttatcgcggc gtttcattcc catccgctgt
    22441 ggctggtgga agagtggctg aatcttttcg gccgggagga aacacaggca ctgtgcgcag
    22501 ccgacaatga aatgccgcct ttgacggttc gcgtcaattt gctgaaatcc ggccgccggg
    22561 aactgatgga aaaactgatc caagccggtt ttcaggtcga ggaaacccca atctcaccgg
    22621 acggcatccg tgtttctttt tctccttcgc ctattcaaaa aaccgctttt ttcgccgagg
    22681 ggcttctgcg ccttcaggac gaggcttccc aactgattgc ctatctggtc aatcctcaaa
    22741 aaggtcaaaa gattctagac gtttgcgcgg gaagcggcgg caaggccacg catctggccg
    22801 ccctcatgaa aaacgagggc cggatcgttg ccctggaccg gaatccggaa aaaatcgagc
    22861 agttgcgaaa caatgcctcg aaaatgggga tcggcatcgt ggaggcggcc cggtcggacg
    22921 gagagattcc tctatcctca gaatatcata atcaatttga cgctgttttt gtggatgctc
    22981 cctgctccgg aaccggcacg ctgcggcgaa atccggaaat caaatggcga ttgcacgcag
    23041 gcgacattga gcggtatgca ggcgcgcaaa aatccattct gcgaaacgcg gcggcggcgg
    23101 tttcccgaaa cggctctctg gtgtatgtca cctgctccct tttatcccgg gaaaacgacg
    23161 aagtggtccg ccaatttcta acggattgtc cgcattattg tctttgtccg ccaaactccc
    23221 atgtggtgca aaacctgatg gatcctcacg gaatattaag gacttacccc catcgcactt
    23281 cgatggacgg ttttttcggc gctgttttcc ggcgccggat ttaatttgac ggattccacg
    23341 cgatcatgct agcttaccaa cccgttacag tgaggagagg aaaccatgtt tattcagcaa
    23401 atgcaggtgg gccatatggc ggttttcgcc tatctggtgg gggaccccgt caccggggat
    23461 gcgctggtca tcgatccggc cgacaacgct catgaaattc tcgccgaagc gtcaaaaaac
    23521 aagctgcgca tcaattacat cgtcaacacg cacggccacg tggaccacat cggcggcaat
    23581 gcccagatga aaaaactcac gggcgccaaa atcatcatcc atgaagacga cgccttcatg
    23641 ctcacctcga cgccggcctc catgcttcga atgttcgggg cggcgcaatc tccgccggcg
    23701 gaccttctcg tttccgacgg tcagatcatt tccacgggca acatcgagct caaagttctc
    23761 cacacgccgg gccattcccc cggcggaatg tgcctttatg cgccgggctg cgtttttacg
    23821 ggcgacacgc tgtttgtgga ggccgtcggc cggaccgatc tgcccggcgg ctcctggcaa
    23881 accatgtatc aggccattaa aacgaaactc tttacgctgc cggacgatac caaagtgctg
    23941 cccggccaca actacggccg caccccgacg tccaccatcg gccatgaaaa attacacaat
    24001 ccatccgttc gataaaggaa aaaagcgacc catgcacaaa acgattgctc agactcattt
    24061 tccaaaactg aaattcgtca aacgcggcaa ggtgcgcgac atctatgccg tgaaagatta
    24121 cctgcttttg gtggccacgg accgaatctc cgcttttgac gtgatcatgc ccgacccgat
    24181 tccgggcaaa ggcgagctcc tgaacaaaat gtccctgttc tggtttaaga agatggaaga
    24241 cattatcggc aatcatgtcg tgtccgcgga tgccgccgat ttccccgagg agtgcgctcc
    24301 ctacgccgaa atcctgcagg gacggagcat gctggttgaa aaagcgacgc ccctgccggt
    24361 ggaatgcatt gtgcggggct acctgtccgg ctcgggctgg caggaatacc gccgcgaccg
    24421 gtccgtttcc ggcatccaac tgcccgcggg cctgaaggaa tcctgcaaac tgccgcagcc
    24481 gattttcacg ccgacgacca aagcgcccca gggcgagcac gactccccga tcacgcggtc
    24541 ggaaatggag gacatcatca gcgccgaaat caccgaacgg atcattcaaa aagccctggc
    24601 catatacgaa cgggcggcgg cgatcgccct ggcggccggg atcatcattg cggacaccaa
    24661 aatggaattc ggactcatcg gcaaaaaatt gattttgatt gatgaattgc tgacgcccga
    24721 ctcgtcgcgt ttctggccgg ccgacgatta cgaagagggg cgcccgcaga aaagcttcga
    24781 caagcagttt ctgcgggatt atctgctgtc cattgtctgg aaccagaagc cgcccgcgcc
    24841 tgaactgccg ccggacatca tcggaaaaac ccgggcaaag tacgaagaag cctattcccg
    24901 tctggtaaaa taaaatatcc aaacccattg acagacgcca taccgttatt acattacgcg
    24961 gtgtttatct tcgcgtcgtc gtgatttgag gaggcatgcc ttggctggaa aaaaactgcc
    25021 ggattaccga ttaaaacaga aaattttgta tatcgacaaa acttctcctg aatcgctcat
    25081 cagttacggg gacctgtact gggaagcggg ggcgccctcc gacgcgctgg acttttacgc
    25141 acgggcgtcg cattcggccg gcctgcagaa aatccgggat gccgcgctgc aaaacggcga
    25201 tgtttttctg ttccatggcg cggccagagc gctggggctg gagcttcggg acgaagactg
    25261 ggaaaacatc gcacggacgg ccgcggcgct tcaaaaattc tctttttccg tacaggcctc
    25321 gaaaaaaatc agcaacgccg aacggacgga cgcgctcgcg gcatggatca aggcggagga
    25381 agcaaagcaa agcgcatgac caagagggac tactacgaaa ttctgggcgt cggcaaaaac
    25441 gccacggatg aagaactgaa aaaatgctac cggaagatcg ccatgcaatg tcatcccgac
    25501 cggaacccgg gcgataagca ggcggaagag cggttcaagg aagcggccga agcctacgaa
    25561 gttttaagcg accggcaaaa gagggaaacc tacgaccagt tcggtcatgc gggcctgagc
    25621 aacagcggat ttcagggttt cagcggattt gacgatgtgt tttccggctt cggcgacatc
    25681 ttcgaggatg tcttcggata cggccggggc cgcggcggca ggcgaacgcg tccggccgcg
    25741 cgggcgggcg ccgatcttcg ctatgatctg aaaatttctt ttctcgacgc ggcgttcgga
    25801 atcaccaccg cgatcgaact tgaaaagctt cacacctgtc atgaatgcga gggaacgggc
    25861 gcggcggcgg gcacaacccc ttccacctgc cccacctgcc acggcagagg ccaggtggtt
    25921 cagtcgagcg gctttttcac catcagctcc acctgccccc actgcaacgg ccacgggagg
    25981 atcattgcca agccctgcca tgcctgccgg ggtacgggcc aggagaagcg gcgcaaaacc
    26041 gtcgaactga aaattcccgc cggcgtggaa accggttcac gcctgcgtct gcgcggagaa
    26101 ggcgaagcgg gagaactggg aggccccagc ggagacctgt acgtctttct gcacgtggaa
    26161 gaccacgaat tcttcttgcg ctcggaagac gatatttcgt gccgcatccc gatctcgatc
    26221 gtgcaggcgg ctctcggcgc gacaattgaa gtgcccacgc tgaaagacac ggaaaaaatt
    26281 aaaattccca ggggtacgca aaccggccag accttccgtc tgaaaggcaa ggggatcccg
    26341 catctccagg gatacggccg cggagaccag atcatcgaag tcttcgtccg aatcccgacg
    26401 gagctgaccc gaaagcagga agatctcctg cgcgaatttg aaaaaagttc tcgacaataa
    26461 agttgcaatc cattccacaa tatgtttaca atgccgcaac ttgaatggcc cggaggaatg
    26521 cgcgatgaaa agcaagatag gtcgtctcgt ggtttgcatg tttgccctga tgtcccttgc
    26581 gggatgcggg gctctggttg ccggcggagc ggtggtcggc gcgggcgcgg gtacgtattt
    26641 gtacgccaac ggcgagctga agacggacta cgcggcctcg ttcgacaccg tctggagcgc
    26701 ctgtgaaaaa acggtcgcgg agatgcgcgg aatacaggtt cagcccacca gggaaattgc
    26761 caagggaacc atttcggccg tcatcaatga tgaaaacgtg aagattgaca ttgcctatcg
    26821 ggcaaaagaa cagacgacgg tcggcgtccg tgtgggcctg atcggcaaca agctgtcttc
    26881 ccaggtgatc cacgataaaa tagccgatca gctggccaaa cgataatgtt ttgcgacgcc
    26941 ccgtcggggc cggcgctggc atcttcatga acaagaaatt tcttttcggc atcctgctgg
    27001 gcgtggtcct gatttacttt tccattcggg gcattcactt caacgacacg cttgcttccc
    27061 tgaagaaaat ccatctcggc tgggcggcgt tgtcgctttt cttcatcgta ttcatgcagg
    27121 ccctgcgatc ctaccgctgg ggcgtcatcc tcgaaccgat ggaaaaaatt tcccagggaa
    27181 cgctctttgc cgtcaccagc gtcggattcc tggcgattgc cgcgataccg gcccggatcg
    27241 gggagctggc ccggccctat ctcatctcga aaaaaagcgg cgtcaaaatg tcatcggcgc
    27301 tgggcaccat cgtcgtggag cgcgtcctcg acagcctggc cgtgctgacc atcaccatcg
    27361 cggttctcat tgcgcagcat gacctgccgc gctggatgtt tacctccggg attcttttct
    27421 ttcttctgac cctgatgatg atcgttttca tcgtggggct ggtctggcgg cgggaaaccg
    27481 ccgtttccgt catcgactgg atcctgcggc ggctgcccgg cgctttggcg caaaaagtca
    27541 atcccctcat ccatcatttc atcgacggct tccaggtcat caccgacgtc aaacggctgc
    27601 tgtccctgct ggccttatcc gccgtggtct ggctggtgga cgtcgccgcg atttacacgc
    27661 tgctcatggc cttcggcctt aatctgccgg tgctggcggc tttcgtggtt atggtgattt
    27721 tgatcgcggg catcgccatt cccaccgcgc cgggttttat cggcaactgg cactacgcct
    27781 gcatcctggg gctgagcctg ttcgggattg ccaaacccca aggcctgtcc ttcgcgctgg
    27841 tctatcattt cctgtcgatg atggtcgtgg tcgttctggg tgcggcgttt ctgccgttca
    27901 ataaattttc gctttcggaa atgacgaagc agatgaagaa gactgaagac tgaagactga
    27961 agactgaaga ctgaaggctg aaggctgaag gctgaaggtt acaaggatgt gcttcgcact
    28021 tgatttggaa taaagaacat aaagggacaa ggcattgcaa tcaatgtctt gccctttgca
    28081 ttttgacctt cagtcttcag cctattcttt taccggcact ttcagggctt tgagtttttt
    28141 gtgcagatta ctgcgctcca ggccgatggc ttcggcggtt ttggagatat tgccgtcgta
    28201 ttcttccagc tttttcaaaa taaactgccg ctcgaaatcg agccgggcat cccggagcga
    28261 atcaccggcc gccgcggtct cttcgaaaga aggcgcgtcc atgtcggctt tgacgctgag
    28321 ttcgggaata tcttccgccc gtatctcatt ggacggcgtc agaatgatca gccgttcgat
    28381 gatatttttc agctcgcgga cgttgccggg ccagtcatgc accattaatt tttccagggc
    28441 ggcggcggtc agggtcttca gcggctgccc ctctttggcg gcaaagtcca ggagaaaccg
    28501 ctccgtcagg tgaatgatgt cttctttccg gtcgcgcaac ggcggcacgc gcaggggaat
    28561 gacgtgcagg cggtagtaca gatcctgccg gaaccggccc gcctccattt ccttctccag
    28621 gtccttgttc gtcgcagcca gaactcttac atccatgtcg atcagcttgt tgccgcccac
    28681 tctttcaaat tttttctcct ggagaatccg cagaatcttg gcctgggcct tcaggctcat
    28741 gtccgccact tcatccagaa aaatcgtccc ctcgtgggcc agatcaaact tgccgcgctt
    28801 cttctccgtc gctccggtga aagcgccctt ttcgtgcccg aacagctcgc tctcgatgag
    28861 ctcctcggga atggccgcgc agttgacttc cacgatcggc ttgtgggagc ggcggctcag
    28921 gtgatggatg gagcgcgcca ccagctcctt tcccgtcccg ttttcgccca tgatcagaat
    28981 ccacgcgttg gtcggcgcga cgatgccgat catttccttg agttcaacga tgaccggact
    29041 ggtcccggtc agttcgtact ggtgcgacac tttctgtttg agcagaatgt tttcttcttc
    29101 caggcggaca aggttcaacg cgttgttcac caggatcatc actttgtcca gcgacagcgg
    29161 tttttcaata aagtcaaaag cgcccagctt ggtggctttc acggccgtct cgatggtgcc
    29221 gtggccggac atcatgatga tcagcatgtt cggatgggcg gcgcggatgg ccttgagggt
    29281 atccagaccg tccatgccgg gcagccagat gtccagcaga acgagctggg gcatttcatc
    29341 ggccacaacc cgaagcgcct cttcgccgct gccggccacg agcacctgat agccttcgtc
    29401 ttccagaatg gccttcagcg attgacagat gctgacttcg tcatctacga ccagaatggt
    29461 ttcgttcatg atgttcaccc atgccgcgtc ttctataaat taaacttcgt tcacgggcaa
    29521 ctgaaaagat acaatcgttc ccgtggggtg gttgtcgctg acggtaacgg ttcccttgtg
    29581 gtccgaaata atggagctga cgatggccag ccccaatccc gttcccgact tcttggttga
    29641 aaaatacggg tcaaacactt ttcgcctgta gtccgccgga accccgaccc cgtcgtcggc
    29701 tacggcgacc accgcttttc tgtgcgcctc atcataccgg atcagaacgc cgacatgccc
    29761 cgtctgcttg ttcagagacg ccacggcgtt gtccagcaga ttcaccatga cccggttgat
    29821 ctgttcggca tccagatgga acctcggcag cccctccgcc ggacggaatt cgaagtgtat
    29881 gtctttgtgc gcgtccacaa aaagcgccac ggcatcggac accaccgcgt taagatcatt
    29941 gggggccagc gtcgtcaccg gcatgcgcgc atagcgggag aattcattca ccagattctt
    30001 cagcacgtcc acctgatcga tgatggtctg tgtgcattcc ttaaaaaccg aaccttcctc
    30061 ccccagctta tccccgtatt tccgctgcag cctctgggcg gagagctgca cgggcgtgag
    30121 cggattcttg atttcatgcg ccatgcgccg ggccacttcc cgccaggccg cggcgcgctc
    30181 cgccatctgc agcttcgtca aatcttcaaa cacaacgaca atgccggagt ccttgccttc
    30241 ttcgtcctca atggtcgtca gggtcagcag aatcgtcaac accttgtccc gcagcgtcag
    30301 ttccagatgc ttttccagaa cccgcatgcg ggtttccttc atttcctgca aaaaggaatc
    30361 gaccagggcg agatgatcct cgtgcaaaag ctcccggtaa ttccggaaca gatacggaga
    30421 agcgtcaatg ccgaacatgg attccgcggc gcggttgatg gtcgtgatgg ttccgttccg
    30481 gtcgatggag atgatgcccg ccgagacatt gcgcagaatg gcggccatgt actttcgccg
    30541 ctcctccagc gacgtattgg cctcgatcag gcccatcttg cttttgtgca aatcatccgt
    30601 catggaattg aaggacttga ccaacaggcc gacctcgtcg tcggcttcaa tgtcaattcg
    30661 gctggtcagg tccccccgcg tgatgcggtt ggtcgccgtt acaaggtcct ggatggggtt
    30721 ggtaatgctc ttggccaacg acaggccgaa ccacgtcgcg agaaaaataa tcgccagcgt
    30781 cacgatggac agggtcacga tgtaattgaa cttgatcgga ttcttcagaa gcttgatctg
    30841 tccgtactgc tccgacgcgt tggcgatgct cctgagcttg tccaccagtc cctggggaac
    30901 gctgttgctg acgtaaaccc gcccgatgac ttccgtcgca acggcatagg agaaaaccgg
    30961 cgccacgccg acaacggcct ccccgccgcg ggcggcggtc accatggata tttcctttcc
    31021 ggaatagatg tcttccagca ttttcggcga cagggtgatg gcggtggacg ccgcctcttc
    31081 gccttcggca aagacaaggc tctggtcatt gaaatcaaaa tacgcttcca gccgagccac
    31141 cttgtagttc ttttgccgct gggaaagaaa atgctccagg taaccggccc ggtccacctc
    31201 gtagaggcgg tttctggtga tttccgcgct gacctgacgg gcgttgaact tggccaggtc
    31261 ctcggcctgc gtgtagtaca cctgcgcgac ttccagcgac cgcgccaggg cgtcgccgat
    31321 tttgatgttg aaccagaatt cgatcgaata ggaaagaaag ttgatggcaa acaaaaaaag
    31381 cagaatggtc ggaaccaggg acaggcccac gaacgcggcc accagcttgg tgcggagctt
    31441 cgagccgatc aatcccttgc gccgctcgga aaacagcttg aagacattgc ggacaatcag
    31501 aaaaagcaga agcaggatca ggatgatgtt gatgctggtc aggccgtaca ccagaatgtt
    31561 cgccgagatc ggcagctctt tctgggcgga caactggctg gccagaatcg taaaaacaat
    31621 cgccaggcag ccgaccacca gcatgatgat gcgctcgcgc cggcgctttc tcaactcccg
    31681 ttcatcaaga tagattttcc tggcgtcttt tttcaacctc atgcgcttgc gtcatcaccc
    31741 ttccccgccc ccgcgatcac tcgtaggcga accggatctt gtacaggggg gtttcaaagt
    31801 cccacaggga cacaaagaac agcacgtatt ccatcttgaa cggcagacgc acacggtcga
    31861 ttttgacccg cacctgcgcg tagtagcgtt cgccgcgggt cagattggaa agggaaacca
    31921 cgggaattcc gttcaactcc gccatcgatt tttgcgcgct ttcaaaattc ggcagaatcg
    31981 ccggctgctc caggccgttt gtggcgatca aaaaattctt tttcaaattg tcgtatttga
    32041 tggtgcggcg gatttccctg cggagaatat gccggtccca cagatagggc ctttcctcat
    32101 agacatccag ctcaatcgta aaaacggcgg ggacccccgc caggatggcg tcctccattt
    32161 cctttttgaa gccgtccgcc agtctcgcgt acaggagcat ttcccccgcg ccgccggtca
    32221 ctaaaatatc cgtcaaccgg ggatccatct gtgcgcttcc cgccggagcg gcagccatca
    32281 tggccatcat gcagcaaccg gtgataaaag ccgtccagaa atgatttttg tgtcttttca
    32341 tgcccgcctg ttttctttcc gaaagaatgc gggtatccta gggaacctgc cggtaaaaat
    32401 caagggtaat ttcggaaaat caaaaaaagg gaatcgggaa caaaatcaga tttgaacggc
    32461 gtccaggatg gtaaaggacg gaatgttgca ggacttgaat tccttcgatt gcggcggatg
    32521 atcttcgctg atgatttccc aacaggattc ctggcgcatg agttcgcgca agagcaggtt
    32581 gttgagccgg tggccggacc ggtgcgccac aaaatggccg atgatgggca tgcccagcaa
    32641 aaacagatcg cctacggcat ccagaatttt gtgcttgacg aattcatcgg gcatgcggag
    32701 gccttccttg ttgatgactc tatcgtcatc aaggatgatc gcgttgtcaa gcgatccgcc
    32761 cagccccagc cccctggcct gcaaaaattc gacttctttc agaaaaccga aggttctcgc
    32821 gcagcaga
//
LOCUS       DAOPAF010000013        31380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1592_length_31380_cov_60.095387, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000013 DAOPAF010000000
VERSION     DAOPAF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 31380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31380
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_31380_cov_60.095387"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLQ77_02620"
     CDS             complement(<1..412)
                     /locus_tag="PLQ77_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09606.1"
                     /translation="MLTLADHILDIAENSVRAGAKLIQIFIEEDSINDLLTLEITDDG
                     HGMAKDVLQKALDPFYTTKTVRRVGLGLPLLRDAAERSGGVFQVNSEENCGTTVKATF
                     GLRHLDRQPLGAIINIIIILIISNSDIDFFYKHRH"
     gene            complement(406..1152)
                     /locus_tag="PLQ77_02625"
     CDS             complement(406..1152)
                     /locus_tag="PLQ77_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPL09607.1"
                     /translation="MLKIYNCDLHIHTCLSPCAELDMHPKALVERALARHLDIIAVSD
                     HNASANVPYVMAAAAGKNIRIIPAMEITTSEEVHLLALFGSLSDLLELQSIVDRHLFG
                     ENDEDRFGVQAVVNERGEVEGLNNQLLIGATDLPIDAIVDIIHGLGGLAVPAHIDRES
                     FSVLSQLGFIDESSHFDALEISKRMGMREGRIRYAELARYPFVTSSDAHFLKDIGTST
                     TPILLKEATFEEIRMAVFGQNGRQILEKQC"
     gene            complement(1323..1664)
                     /locus_tag="PLQ77_02630"
     CDS             complement(1323..1664)
                     /locus_tag="PLQ77_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09608.1"
                     /translation="MNIEAIGAALGLEVKCGRESLGKNVTGGYTGDLLSDVMGNAKEG
                     YIWITRQVHQNIVAVASLKELAGIILINACEPARETLEKAEAEKIPILVSDLPAFELS
                     GRIYNLLTSGS"
     gene            complement(1681..2124)
                     /locus_tag="PLQ77_02635"
     CDS             complement(1681..2124)
                     /locus_tag="PLQ77_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09609.1"
                     /translation="MGETKANLYSESFQIEGGNFNSAGIVSTSIKSILKKLGIPGDIV
                     RKAAIVSYEAEINVVSYAKKAVVNLTVKPGAVLIDVIDEGQGIPNIDLAMQEGYSTAD
                     HRIREMGFGAGMGLCNIKNYSDDFHIASEVGKGTHLKMTIQTPST"
     gene            complement(2111..2485)
                     /locus_tag="PLQ77_02640"
     CDS             complement(2111..2485)
                     /locus_tag="PLQ77_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPL09610.1"
                     /translation="MKLSEVRDILDAEIIVGEDCLDLEVHTAFGADLMSDVLAFAKAG
                     SLLLTGLTNTQVIRTANVLDIAAIILVRGKKPSSETITLASELKIPILATKYILFETA
                     GRLYMKGIVGCLPKVETTSGRN"
     gene            complement(2601..3656)
                     /locus_tag="PLQ77_02645"
     CDS             complement(2601..3656)
                     /locus_tag="PLQ77_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPL09611.1"
                     /translation="MKKQRKFCVYCGGPVAKKKEDSVWRDFCSECRSFFYDNPLPVVS
                     AIVDVSREILLVKRDRAPFQGRWCLPTGFAEAGENIEEAVLRELGEETGIRGRISRLL
                     DVDSYKSRFYGDLLFLTFVVEKTAGKVAAGDDSAQAKFFPIDRIPPLAFRSNRRAVTA
                     YIQSKKDYWAILDAVDKRSNPSLRNAKRANSLTRRLIGNLLKNREAIIRQWIEDVSAN
                     PSTAEYRRYEPPVLHGICDRIISQITLWMGDAYGAPHIRNFYIKLGRERKKAGTLLSS
                     ALSALSLIRKHIWDVALARGALQKNLGLYATFELQRRMTIFFDLAAFYIARGYESSKT
                     EPRIPGAASSKRKRRAI"
     gene            complement(3653..4513)
                     /locus_tag="PLQ77_02650"
     CDS             complement(3653..4513)
                     /locus_tag="PLQ77_02650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09612.1"
                     /translation="MADPSDDKKIREVFRDVLNHREMERIIRRCSTNPDDIRQTALAG
                     ADLTSCRNVLELGCAFGSFTGALKCRLHPRARIAGLDIIPEYEPFFLEACAKAGYAGR
                     FSSDGVHQIAGFPPSDADLILCSFALYFFLDVLPDIARILKPDGLFITITHASGNMRE
                     LIDVIRSILKARRIIQPDDVLPIEEILKAFSAENGKALLAPHFGKIRQIDFPNTLVFP
                     PQDIGAFIDYYRFKRPFFLAGTPPAADIILPDLTRALAGMAAGRQTLSMCKDDAIFIC
                     SHPRSSKEAP"
     gene            complement(4575..5543)
                     /locus_tag="PLQ77_02655"
     CDS             complement(4575..5543)
                     /locus_tag="PLQ77_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09613.1"
                     /translation="MMRVYFWGTRGSLPASITAGAVEEKIVKAIRASRTHSLETDEAI
                     EAFVHNELPFPVRASYGGNTACVELCGAEEYVLCDAGTGLRDFGGAYMKAKAAGQPGL
                     PNVFHLLMSHPHWDHLQGFPFFTPAYLPGHRIHIYGGHADLERIFIRQQSEPCFPVGF
                     HAMKADIRFHTLEPESEYDIAGMKVACIRQNHPGDSYGYRFEKDGRTVVYSTDSEHKD
                     GGREEYERFLSFFSNADLLIFDAQYRFLDAVDTKENWGHSSNLLGVEMAAAAGVRRLC
                     LFHNEHTFDDDALDRFLEDTRAYLKILDGSSSLKIDLAYDGLDIEL"
     gene            complement(5645..7333)
                     /locus_tag="PLQ77_02660"
     CDS             complement(5645..7333)
                     /locus_tag="PLQ77_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09614.1"
                     /translation="MKKKILMVDDEPGSLFLLEALLNANGLETAMAENGKEALAMARL
                     NPPDLIVSDILMPVMDGYTLCREWKLDETLRSIPFVFYTAEYTRSRERALALSLDPDD
                     FILKPQEPHILIRLLIELLEKRKPAKPKIPGPAKEEIEFLRKHDKILFGKLEKKMADL
                     EKANEELREQEEKYRLSFEQVSDVIFILGTDLRILSISPSVERNLGYHPLEFVGKPFA
                     KIKKVMDPGCYAQASAKLASILEGKTVEPSDYIIFAKDGTLRHIETSATPVVKNGKTI
                     CIVCVTHDITQRKRAEESLINERNFSRTVAESLPGLFFVLDERGKFLRWNDNLRAVTG
                     YADEEIREMLFADLCPQSHRNAALEGVKEILRAGELSSEAELIFKDGTRRIFRLNARR
                     LSDEDRLCIVGAGMDVTDRKKAENELKRFAESLEEANIALRVLMHQRDEDQKDIEEKL
                     QANVHDLILPCLKKLKGANLEDRYKNDLQTLEYNLREILSPFLKNILAAHADLTPREI
                     QIMDLIKQGKKTGEIAAALNASLKTVETHRNHIRKKLNLVNAGVNLRSYLLSVS"
     gene            7681..8583
                     /locus_tag="PLQ77_02665"
     CDS             7681..8583
                     /locus_tag="PLQ77_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09615.1"
                     /translation="MDDCRVQLANLVDSRDPKAVLEEIHVIAGLMVPRFDFAPFDRAY
                     SDVVRLFRGEYPSYRSCNVQYHDLGHTLSVTLAVARLMHGAVEAGQSLTEKDLNMGLI
                     SGLMHDTGYIQEIADRQGTGAKYTQVHISRSISFLQAYYRGNPAFQNDLDSFRDILLC
                     TGLNTRVDAIRFAGATVELLGKILGTADLLGQMADRYYLEKLPELYEEFAEAGITTFS
                     SRLDLLDKTQGFYRMTLKRFEDELGNVQRFSRCHFRKRWGIDEDLYIQSIEKNMAYLA
                     RVVSLHRENYADHLRRFAHTKEHL"
     gene            8602..9417
                     /locus_tag="PLQ77_02670"
     CDS             8602..9417
                     /locus_tag="PLQ77_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPL09616.1"
                     /translation="MKRIVCLLTILLLAAGICGAAAAEDSLQNIKKRGVLRAGVKDLT
                     PSFALADIKTGEISGYDVDFVRAIARRLDVRLEITPFSGEENERISFLTEGKVDIVAA
                     TMTITREREKLIDFSHPYFVTAQKFLVHKDTVQRLSDLKNKQIGTVDQTTSVENLRKA
                     LPSASIVLFTGYVPAFLSLQNRQIFAVSTDEAILAGILAKSDKDVFEIPKVSISREPY
                     GLGVRKGQTSFLRFVNQALLEMEHSGEAKRIFDKWFGPESSAPLERTFRITAK"
     gene            complement(9462..9908)
                     /gene="rpiB"
                     /locus_tag="PLQ77_02675"
     CDS             complement(9462..9908)
                     /gene="rpiB"
                     /locus_tag="PLQ77_02675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPL09617.1"
                     /translation="MKKIVIAGDHGAVALKDRIIAFLQRKNFEVTDLGTWGSESVDYP
                     DTAAAACREFLKGGYEFGILLCGTGIGVSIAANKIAGIRCALIGDPFSAEMARAHNNA
                     NFIAFGGRVRYAVPVEEMIEKFLSTPFEGGRHERRIAKIAALENSR"
     gene            10016..10903
                     /gene="xerD"
                     /locus_tag="PLQ77_02680"
     CDS             10016..10903
                     /gene="xerD"
                     /locus_tag="PLQ77_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPL09618.1"
                     /translation="MREWLNRYYQYLLIEKGAADNTLAAYGRDLNRYLAFLEQRGLSN
                     PWSALPQTVVDFLVQIKSEGLSANSANRTLAALRGFYKYLLREKVLEESPLAQIELAK
                     VWMRLPDTVSSDEMKKILSQPDDSTPQGLRDRAMLELLYASGLRVSELAGLTMNSINW
                     QVGFLTVMGKGGKERVVPVGKTAYDCVRRYVDEARPKLIRSKTTDVLFLNRFGTSLTR
                     QGLWKIIVHYAQKAGLKKNVHPHTFRHSFASHLLEGGADLRAVQVMLGHSDISTTQIY
                     THVTRERLKEIHRKYHPRG"
     gene            10908..12899
                     /locus_tag="PLQ77_02685"
     CDS             10908..12899
                     /locus_tag="PLQ77_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL09619.1"
                     /translation="MRKPAGLKIGFGKKVFAAAGITALVLALLTVFFPKTFQTAGFYL
                     QRGINGFSYYILGASPHFYHLEMEKNASDFRVRAGETLDVTYRDEFVVKSVASDDWSG
                     KSLEPVLEGPGGKAGIIGVPFRGIDLVDKLLAGGVIPEGTQAAGVYQIAVYSTRDKIG
                     VVPLRVMITPQDWLRFAKSRGNPGEQEAYLKKAAAQNREDAGVRKILAGIYLQQNRIG
                     EAAKLYEEILRISPGDPLAMKELARCIAQLGREDRAIDLLSRLTEVQTEDAEAHAMLG
                     AAYGRKTIWDKAADSYFRAVQIDPDNHAVRLLLAQAYEKSRRTDAAVEQYRFVIRHAP
                     DAAPAWRAMGDMHLKSGRYAQAIESYTRVLDLQPKDAAAHANLATAYAGLGKAREEME
                     HLQKAAALSPDEPVIRFNLAAAYEKRGKTPDAVREYGRVLKNKPDDADALARLADLTF
                     QNKQYEQAAGYYEKLSRIHPTRASIFVRLGFARGELKQYAASAEDYEKALRLGAKDQA
                     LYYNLAFTYTKLGREKDAVRYYEKISPQNKKTLSILADHYLREKQYAQAIRYYQRILK
                     LEPKKASSWAGLGYAWLASGNPDKAIEHYSQALKYDREDDEIYANLGIAYEKKGLYPE
                     ALKAYRSAYEINPETRVAPRIPRLRIQLLHKKDNRGSEP"
     gene            12896..13729
                     /gene="kdsA"
                     /locus_tag="PLQ77_02690"
     CDS             12896..13729
                     /gene="kdsA"
                     /locus_tag="PLQ77_02690"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPL09620.1"
                     /translation="MKQVKLNSHTIGGKAGFILIAGPCVLENEASAMEIAVYLKELTD
                     RLEIPFVFKASYDKANRTSVAAYRGPGARAGLAILKNVREKIGVPVLSDVHRFEEIEE
                     AAEVLDVMQVPAFLCRQTDFVVEVAKKAKIVNVKKGQFLAPWDVANIIGKIQSAGNEN
                     ILITERGASFGYNNLVFDVRALPIMRAMGYPVIFDATHSVQLPGGMGTSSGGQRDMVP
                     YLARAAVAAGVDGIFMEVHPDPEKALCDGANSLYLNSLKELLTTLKKIDGITKGIGHG
                     S"
     gene            13886..14425
                     /locus_tag="PLQ77_02695"
     CDS             13886..14425
                     /locus_tag="PLQ77_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPL09621.1"
                     /translation="MKKKIRGKLEKIKMLILDVDGVMTDGRIIMDSDGREIKNFYVRD
                     GHGLVMLQRHGIRVAILTGRTSSVVDHRARELKIAQVYQGALNKKEVYAKILEKNGLA
                     PEETAYLGDDIVDIPVLKMAGFSAAVADAVDEVKKHVDYVTLCRGGKGAVREICEMLL
                     EALGFWPEVARRYDFDGPE"
     gene            14504..15016
                     /gene="lptC"
                     /locus_tag="PLQ77_02700"
     CDS             14504..15016
                     /gene="lptC"
                     /locus_tag="PLQ77_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPL09622.1"
                     /translation="MILVLAGAVLFALLRATGGKKETIVKILPDEADVRIADFFYTEV
                     GKDNLRWEVKAKTAQYQKKRNLALFDQVQMKLITQKGRTFVMTGDQGEMRTDSKDVEI
                     RGHVVATADTGEKFSTEVLRYSDARKQIYTDASVRMETSRMKIQGVGLSILMDKGELA
                     LSSGVKAKIY"
     gene            15030..15575
                     /gene="lptA"
                     /locus_tag="PLQ77_02705"
     CDS             15030..15575
                     /gene="lptA"
                     /locus_tag="PLQ77_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HPL09623.1"
                     /translation="MKKIVLITAGILVALNLQAWAQTGLLQKNTLRKNEPIQIVSDKM
                     EAFQATRMVVFSGNAVAQQGDVRIQADRLSIYYKNSAAQSEKVGPDAEQTGDLEKIVA
                     SGHVVITQKDVLATGNEAVYYQDTARFVMTGNPVLRQEKSVIKGCRVIIQVDENRGKV
                     EQCADENSGRVTAIIHPRKKK"
     gene            15605..16327
                     /gene="lptB"
                     /locus_tag="PLQ77_02710"
     CDS             15605..16327
                     /gene="lptB"
                     /locus_tag="PLQ77_02710"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPL09624.1"
                     /translation="MSHLSARGLVKIYNKRKVVNHIDLTISPGEVVGLLGPNGAGKTT
                     TFYMMVGLVKPDGGQIFLDDEEIGAYPMYRRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLQISEEERRRRLEELLGELDLTTLAKNRAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQNIIEKLKSKGIGIIISDHNVRETLACCDRAYIINEGAILVEG
                     RPDVIARSELAKKFYFGEDFSL"
     gene            16355..17827
                     /gene="rpoN"
                     /locus_tag="PLQ77_02715"
     CDS             16355..17827
                     /gene="rpoN"
                     /locus_tag="PLQ77_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPL09625.1"
                     /translation="MAFELRQNLKLSQQLIMTPQLQQAIKLLILSRQELVDTINQEME
                     ENPLLEEVAPDDESAEELHAEVAEDVQSVEKEESKIPEHTAELTGEGDGREEFDWNNY
                     LEDYGPVGVSYDRAEGDAPTLDNVVTESPSLTEHLMWQMKLSPFNGEEVRVGEQIIGN
                     LDSNGYLRATVEEIARLENVAEEFVEKVLKKVQEFDPAGVAARDLQECLLIQAGMFAR
                     ENPLIEPIIREHLKELELKNYLQIARRLKVPLKEVETAVLLISKMDPKPGAVYSEEKA
                     QSIIPDVYVVKSGDDYKIILNDDGLPRLRISNFYREIMGGVGGPDSAEKENGRKYIRE
                     KVQSATWLIKSIQQRQKTIYRVAESIVKHQKEFFDKGIDYLKPLVLRNIADDVEMHES
                     TISRVVTNKYMHSHRGIFEMKYFFGSSIQRTSGENIASKSVKEEIRQIIGKEDQKKPY
                     SDCEIVRLLKARNIDIARRTVAKYREMTGILPSSKRKKYF"
     gene            17984..18514
                     /gene="raiA"
                     /locus_tag="PLQ77_02720"
     CDS             17984..18514
                     /gene="raiA"
                     /locus_tag="PLQ77_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPL09626.1"
                     /translation="MNISVTFRTGEGENWQKEYAEEKIARLKKYLDVPADVHMILSTE
                     KFRNMVEINLTANGWNVNAKEEAKDMRLAVDRCIVKIENQLKKQKEKSRQHKPKSIRH
                     AGDKALEGAEEEDAASKVVETRKIILKPMSLEEAVLELESGKARFLIYRDTASEKVSL
                     VHRLDDGQLAHIETNS"
     gene            18620..19087
                     /locus_tag="PLQ77_02725"
     CDS             18620..19087
                     /locus_tag="PLQ77_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPL09627.1"
                     /translation="MRLDQIFKMEFLSENLLSDTKEGVLAELISLLIGSGLKIDRAKA
                     LDVLQQREKLGSTGIGDGVAIPHGKVSELQDMVIAFGRSRRGVAFDAIDGKPVQLFFL
                     LLAPEQSTGQHLKALARISKMLKTPNFRKTLVDAKNRADLYKAIVEQDATCPL"
     gene            19095..19949
                     /locus_tag="PLQ77_02730"
     CDS             19095..19949
                     /locus_tag="PLQ77_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09628.1"
                     /translation="MPHPEASAREWFRDYLPYLGIRTISGPRNHKNPFPAGAVQCLRS
                     PTAFPRREAAILLFPPETANKLLALSGPSREEYFSRMAGRRPVLLIFSQSPSLPAVIR
                     NQLRNHCLWAAASPLHENLLASRIRSILLEKIHQCMAMHGVALACGDRGVLLTGPSGI
                     GKTTAALETAREGCFWIADDLAVIKNVRGRLMISGHPRIQKYVHAGEKGIEFANRVLP
                     ARRTKQRAELFGIVDVCRSAGKKISRPIEIRILEKRLPLVRVCISRTGYLRQNLLTRA
                     MQKLNEVG"
     gene            19950..20816
                     /gene="rapZ"
                     /locus_tag="PLQ77_02735"
     CDS             19950..20816
                     /gene="rapZ"
                     /locus_tag="PLQ77_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPL09629.1"
                     /translation="MKNLQVVIITGLSGSGKSTALRALEDIGFFCVDNLPVVLLPKFL
                     SITLQSSPKIRQVAMVMDLRQKSFLEKYQRIFERLKEKGYRINILFLEAGEESLLRRF
                     SETRRSHPLSEKRSIMEGIQEEVKKLSTLRQMADTVIDTTTSNVHQLKDTVQKQFLPS
                     STRKKMMISVMSFGYRYGLPADADLAIDVRFLPNPYYVEKLKNLNGHHRDVEKYVLNN
                     EECKTFMNKAADMLNFIIPLYEKEGKVRLNVAFGCTGGKHRSVVMANQFSLFFQKQKF
                     IVHTTHRDINKS"
     gene            complement(20961..22283)
                     /locus_tag="PLQ77_02740"
     CDS             complement(20961..22283)
                     /locus_tag="PLQ77_02740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL09630.1"
                     /translation="MKKTGIIAAAVVIVLAAGALGWLYFAGWLDRDKPAIRLDSDITA
                     IGKKKDISIAFSDERSGLSGLKVEIVQDNKPRLLAQESFPSRSTRQKVLRLTVEPLLL
                     QLNNGPAVVRITAGDASLFKNETVISEQVRIDMMPPQVYVMSPVNYINQGGAGFIPYR
                     TSKPAALTGVFVDDRFYPGYNIVIGGKPTSVAFFALGTGASAATKIVIFARDNAGNEA
                     RTSMPFTIKPKKFRADKVNLSETFLQKIMPEFQASIPQLQGKTPVEVFGYVNTTLREE
                     NAKTIQSVCRKSINKKLWEGAFHRMRDAAPMALFGDQRTYLVDGKPFGNSVHLGVDLA
                     STAHAPIEAAANGIIVFAGALGIYGNTVIIDHGLGLFSLYGHLSAIETTVGKSVKKEE
                     KIGVSGLSGLAGGDHLHFSILVGGEFVNPQEWWDPHWIEDNVMKKMNL"
     gene            22458..23672
                     /gene="coaBC"
                     /locus_tag="PLQ77_02745"
     CDS             22458..23672
                     /gene="coaBC"
                     /locus_tag="PLQ77_02745"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPL09631.1"
                     /translation="MLKNKKIVLGITGGIAAYKSAELTRAFIKAGAEVKVIMTQSATE
                     FIAPLTLRTLSRNPVYADMFEDAEPYDMAHIALAEWADAFVVAPATGNVIGKIASGIA
                     DDLLTTTLMAENRPTLICPAMNDKMLANAFVQENLRKLAGAGYRVMQSGVGELACNAA
                     GAGRLPEIPEILDEMEALLTVQDFAGEKVLVTAGPTQEPLDPVRYITNHSSGKMGYAL
                     AKAAHQRGAQVTLISGPTRIVPPKVETVVFVRTAQEMHRAVMDAWTKASVIIKAAAVA
                     DYRPRVAAGEKIKKDRGLSGIELVRNPDIIADIGAKKKKGVLVGFAMETEDLLDNARR
                     KMIRKNMDMIVANNLRDDGAGFEGDTNIITILDRLGHTVALDKMTKQDAAHRILDHIR
                     SFKSKKGPAGKK"
     gene            23669..24400
                     /locus_tag="PLQ77_02750"
     CDS             23669..24400
                     /locus_tag="PLQ77_02750"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPL09632.1"
                     /translation="MTQSFPPSSSDEIRNELLSVVKSVRGQVLDAERLYVALDCKRPQ
                     PARKNTPDGSDRGEALHSLSLEAANCRQCPLAQTRKNVVFGAGSLSAQVVFVGEAPGA
                     DEDEQGQPFVGKAGQLLTDIIVKGMKLTRKDVYICNILKCRPPGNRNPLPEEIRLCEP
                     FLKKQLQLIRPRVICALGKFAAQVLLKTETPISALRGRFQLYEEIKLMPTYHPAYLLR
                     NPSAKKQVWEDVQLIMKEIGLGGPS"
     gene            24417..25727
                     /locus_tag="PLQ77_02755"
     CDS             24417..25727
                     /locus_tag="PLQ77_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPL09633.1"
                     /translation="MLKKTGILFMILLWIASAGVVPAEEARPLFHVIELGGVITSPSA
                     KYVARSLEEATAAKAEGLIILLDTPGGLDTAMRDIAKSILNAPLPVIVYVYPSGARAA
                     SAGVIIAQAAHVAAMAPGTNIGAAHPVAIGLGGSGEMDKTMSQKVENDAAAYARSIAK
                     TRGRSEEWVEKAVRKSESITADEALKLNVIDIVAVDIGKLLAAIDKRTVTVAGAKKKI
                     STAHAVLDTKKMGTRQGILAAISNPNIAYLLLLIGLAGLYFEFSTPGAILPGVIGGIS
                     LLLAFFALSTLPVSYTGILLILFSVILFIAEIKIMSHGMLTVGGVISLALGSFLLFDS
                     PEPALRISLQVLLPAVLVASGFFIVIIGLAVKAQMRKHYSGTEAMIDGRGEAATDIHG
                     DGKVFFQGEYWTATSDREIPKGAPVRIIKVEGLKLVVEEIKEGK"
     gene            25753..26517
                     /locus_tag="PLQ77_02760"
     CDS             25753..26517
                     /locus_tag="PLQ77_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPL09634.1"
                     /translation="MLGSLPLPLLVVIVLVVMFLASAIKILNEYERAVLFRLGRLRDI
                     KGPGLIVIIPGIDKIVRIDMRTITMDVPPQDVITKDNVSIKVNAVVYFRVLDANAAVV
                     NVENYLFATSQLAQTTLRSVCGQVELDEILTEREKINLEIQEILDRSTDPWGIKVSLV
                     EVKHIDLPEEMKRAMAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMGTQPMSLQL
                     RYLQTLNQIAAENNSTTVFPIPIDLFKPFLNLAGKS"
     gene            26581..26892
                     /locus_tag="PLQ77_02765"
     CDS             26581..26892
                     /locus_tag="PLQ77_02765"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPL09635.1"
                     /translation="MPNEKRNILTGGLILITLGVLIFISKTTSYSFGQTWPILLIVIG
                     ITTLFYSIRAVEGWFVATVGVIFLIIELYGFDLYKYSQYILPVILIVLGVFVLLKRRK
                     R"
     gene            26945..27583
                     /locus_tag="PLQ77_02770"
     CDS             26945..27583
                     /locus_tag="PLQ77_02770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HPL09636.1"
                     /translation="MVLSDFDGTISRVDVGDGVLSRFASESREAIDSAYIRGGMGSRE
                     AYGKIAPLVRVDAGRLRAYVLKCSAIDPYFSRFYRLAAKKGVDVKIVSDGLDFYIRAI
                     LDKHRLGEIEFYSNTVAFGGEDTLTFDFPQANALCGRCGTCKSKILHDHRLMYEKIIY
                     VGDGHSDICPSQYADLIFAKGELLKKCRRESAVPYRAFHDFREVSEYLKKNV"
     gene            27627..28811
                     /locus_tag="PLQ77_02775"
     CDS             27627..28811
                     /locus_tag="PLQ77_02775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPL09637.1"
                     /translation="MSISKKIKASMSQSSLIRKMFEEGNLLKSRHGHDKVYDFSLGNP
                     NVDHPREFKKELIRVARQEIILKHGYTPNAGYPETRRAVAQTISRSIGLKMDADHIVM
                     SCGAGGALNVILKSILDPGDEVLILKPFFVEYPFYIENYGGVVKIADTRPDFSPDLEA
                     IARMITRRTKALILNSPNNPTGRVYSKTNIAGLARLLREKEKKYSKAIYLLSDEAYAQ
                     ILYDGIQLPSVLTAYADSIVAYSYSKTLSLPGERIGYIAVNPKIHEADDLIRALILCT
                     RIMGFVNAPALMQRIVANLQHITVDVKIYQKKRDLLCGGLARAGYQFIKPQGAFYLFV
                     KSPVPDDVAFAQRLVQKNILVVPGSGFGTPGYFRIAFCVDDATIRNSMKGFAAAIRDC
                     RG"
     gene            complement(28991..>31380)
                     /locus_tag="PLQ77_02780"
     CDS             complement(28991..>31380)
                     /locus_tag="PLQ77_02780"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HPL09638.1"
                     /translation="IAVIAAALSIQDPRERPYDKIDQAAAAHAQFAHPESDFLAYLQI
                     WNRYSDYFEQMQPQGQRRKFCRDHFLSYRRMEEWRDVYSQILDILGGKAGAISRRKPV
                     RAEIDQEVSDKIHRSILSGYLSNIAQKKEKNFYTAAKSREVMLYPGSGLFNRGGPWIV
                     AAEISLTSRVFARNAANIQSEWLEELGGEHCRCQHWAAHWEKSRGQVVALEKVVLFGL
                     TIVEQRSVAYERINPEEAWRIFIREALVTGETGRKLPFLEHNLSLREKVKTMEDKLRK
                     RGIAADEETIAGLYEQRLPLLSDIRSLQKLIKDRGSDAFLRFREEDLIAAGPDLDELN
                     RYPDQIRMGNAVLPCRYKFEPGRAGDGVTVRMPLGLIAGASPVNIEKHLPSLREEKAL
                     HWLKSLPKNIRRNLPPLIEVAGRFNRQTAGEDQALPLALSRFLFDEYHLTVPAGFWAP
                     EKLPPHLQIRYSVIDDRGSEIRSSRDIGELQRELTEEIRRTSLEDCRRQWEKENLTSW
                     DFGDLPENVEIAGKHGVTGLAYPALQDQGGAVHLRIFADPREAAAVHAAGVAALYAIH
                     FEDRIKQLKKNISLGGELKEQASLLGSPRQVERSVLERVKRDLFRRSWRSPKEFMKHA
                     DEVRPKILSYGQEVVAAIAPVLRSFAETHDGLQKLIFKNRPHPPLAEFLQATLAEAHV
                     LVPEDFILHCDFDRLRHLPRYLKALFLRAERGSLNLASTESKLRQAATYAVKLQEIRG
                     AMDTDASEEKRNKTDELRWMIEEYKVSLFAQELKTPYPVSPKRLNQLIQEIGNML"
BASE COUNT         7045 a         8388 c         9210 g         6737 t
ORIGIN      
        1 tatgcctgtg tttataaaaa aaatcaatat cagaattaga aataatgaga ataattatta
       61 tgttaattat agcacccaga ggctgccggt ccaaatgacg caggccaaac gtggccttta
      121 ccgtagtgcc gcaattttct tcagaattta cttgaaagac accgccggag cgctccgcgg
      181 cgtctctcag caggggcaga cccagaccca cgcgacggac cgttttggtc gtataaaacg
      241 gatccagcgc tttttgcagg acgtccttgg ccatgccgtg cccgtcgtcg gtgatttcaa
      301 gcgtcagcag atcgtttatg gaatcttcct caatgaagat ttgaatcagc ttcgcgcccg
      361 cgcgcacgga gttttcggcg atgtccagga tatgatcggc cagcgtcagc attgcttctc
      421 cagaatttgg cggccgtttt gtccgaagac ggccattctg atttcttcaa aggtcgcctc
      481 tttcagaagg atgggggtgg tggatgttcc gatgtccttt agaaaatggg cgtcggaaga
      541 cgttacaaag gggtaccggg caagctcggc ataccggatg cgcccttcac gcatgcccat
      601 gcgtttggag atttccagcg cgtcaaaatg cgagctttcg tcgatgaagc cgagctggct
      661 cagcacgctg aagctttccc ggtcgatgtg ggcggggacg gcgagtcccc ccagcccgtg
      721 aatgatgtcc acgatcgcat cgatgggaag atccgtagcg ccgatgagga gttggttgtt
      781 gagcccctcc acttcccccc gctcgttgac gacggcctgc acgccgaagc ggtcttcgtc
      841 attttctcca aacaggtgcc gatcgacgat cgattgaagc tccagaagat cggacaggct
      901 cccgaagagc gccagcaggt gcacttcctc gctggtcgtg atttccatgg ccggaataat
      961 gcgaatgttt tttcccgcgg cggccgccat gacatacggg acattggcgg aagcgttgtg
     1021 gtcgctgacg gcgatgatgt cgagatgccg cgcgagtgcc cgctccacca gggccttcgg
     1081 atgcatgtcc agctcggcgc agggcgacag gcaggtgtga atgtgaagat cgcagttata
     1141 aattttcagc atggcttctt gcatcgctcc tggcggaagc gcccctgagg gggatcgcct
     1201 gaaggtcgcg gggcagaggg gggaaggggg gctgggtttt cgcttcggcc cctttcccgg
     1261 gcggggaaaa gaaggcgcct tgcaggaatc attcgccgcg aacggccggg cggcttcggc
     1321 gatcagcttc cggatgtcag cagattgtaa atccggcccg agagctcaaa ggccggcagg
     1381 tcggacacca ggatgggaat cttttccgcc tcggcttttt ccagtgtttc cctggccggt
     1441 tcgcaggcgt tgatcaggat gatgcccgcg agctccttga ggctggcgac ggcgacaatg
     1501 ttctggtgca cctggcgggt gatccagata tagccctctt tggcgttgcc catgacatcg
     1561 cttaagaggt ctccggtgta gccgccggtg acgtttttgc ccaggctctc ccggccgcac
     1621 ttaacctcca gccccaaagc cgcgccgatt gcttcaatgt tcatacacta accctttatt
     1681 tcaagttgat ggcgtttgaa tggtcatttt caaatgcgta cccttgccga cctccgaggc
     1741 gatgtggaag tcgtcggagt aatttttgat gttgcaaagc cccatacccg cgccgaagcc
     1801 catttcccgg attctgtgat cggcggtgga gtatccttcc tgcatcgcca gatcgatatt
     1861 gggaatgccc tggccttcgt cgatcacatc gatgagcacc gcgccgggct tgaccgtcag
     1921 gtttacgacg gcttttttgg cgtaggatac aacattgatt tccgcctcgt acgagacgat
     1981 ggcggctttc cggacgatgt ctccgggaat gcccagtttt ttcagaatgg atttgatgct
     2041 ggtcgacaca atgccggcgg aattgaaatt gccgccttca atctgaaaac tttccgaata
     2101 gaggtttgct ttagtttcgc ccactggtgg tttcaacttt tggcaggcag ccgacgattc
     2161 ctttcatgta gagacgcccc gccgtttcaa agaggatgta ttttgtggcc agaatgggga
     2221 ttttcagttc gctggccaaa gtgatcgttt cggaagacgg ctttttcccc cgcaccagaa
     2281 taatggcggc gatgtccagc acgttggcgg tccggatcac ctgggtgttg gtcaggccgg
     2341 tcaagagcag gctgcccgcc ttggcgaaag ccagcacgtc gctcatcagg tcggcgccga
     2401 aggctgtgtg cacttccagg tcaagacagt cttcaccgac aatgatttcc gcgtccagaa
     2461 tatctctcac ttcactcagt ttcaaaggct gctcctcgtc aatccgcaaa atggtttaca
     2521 gggatccaac agttcccgcc gctttcgttt ccggaaaacc gcagtctttt tatcatggct
     2581 tatgaccttc gtcaataaga ttaaatggcg cggcgctttc gttttgaaga ggcggcgccg
     2641 gggattcggg gctctgtttt cgatgactcg taaccccgtg cgatgtaaaa agcggccagg
     2701 tcgaaaaaaa tggtcatgcg acgctgcagc tcgaaggtcg cgtaaaggcc gaggttcttc
     2761 tgcagcgctc cccgggccag cgcgacgtcc cagatatgct tccggatcag gctcagcgcg
     2821 ctgagcgcgc tgctgaggag cgttccggcc ttttttctct cgcggcccag cttgatgtag
     2881 aaattccgga tgtgcggcgc tccgtaggcg tcgcccatcc agagcgtgat ctgcgaaata
     2941 atccggtcgc agatgccgtg gagcaccggg ggttcgtagc ggcggtattc tgcggtggac
     3001 gggttggcgg acacgtcttc gatccattgc cgaatgatgg cctcccggtt tttcaaaagg
     3061 ttgccgatca gacgccgggt cagggaattg gcgcgcttcg cgttgcgcag cgatggattg
     3121 gagcgcttgt cgacggcgtc caggatggcc cagtagtctt ttttgctttg aatgtaagcc
     3181 gtgacggcgc ggcggttgga gcggaacgcg agcggcggga tgcggtcgat cggaaaaaac
     3241 ttcgcctgcg cgctgtcgtc gcccgccgcg actttcccgg cggtcttttc cacgacaaat
     3301 gtcagaaaga gcaggtcgcc gtaaaaccgg cttttgtagg aatcaacgtc cagaagcctg
     3361 ctgatccggc cccgaatgcc ggtttcctcg cccagttcgc gcaggacggc ctcttcgatg
     3421 ttttctccgg cttccgcgaa tccggtcggg aggcaccagc ggccctgaaa cggcgcccgg
     3481 tcgcgcttca ccagcaggat ttccctcgag acatccacga tcgccgagac caccggcagc
     3541 ggattgtcgt aaaagaacga gcggcattcg gaacagaaat cccgccaaac cgaatcttct
     3601 tttttcttcg cgaccgggcc gccgcagtac acgcagaatt tccgttgttt cttcatggag
     3661 cttcctttga tgaacgggga tgtgaacaga tgaaaatcgc gtcgtccttg cacatggaca
     3721 gcgtttgacg gccggccgcc atgccggcaa gcgcccgggt cagatcgggg aggatgatgt
     3781 ctgccgccgg cggcgttccc gccagaaaaa acgggcgctt gaagcggtag taatcgatga
     3841 acgccccgat gtcctgcggg ggaaaaacca gcgtattggg gaaatcgatt tgccgaatct
     3901 tcccgaaatg aggggccaga agcgccttgc cgttttccgc cgagaacgcc tttagaattt
     3961 cttcaatggg caggacatcg tccggctgga tgatccggcg ggccttcagg atgctccgaa
     4021 tgacgtcgat cagttcgcgc atgtttccgg acgcatgcgt gatcgtgatg aataggccgt
     4081 ccggcttcag aatacgggca atgtccggaa gaacgtccaa aaaaaagtaa agggcgaagc
     4141 tgcagagaat cagatcggcg tccgacggcg gaaagccggc gatttgatga acgccgtcgc
     4201 tggagaaccg cccggcatag ccggcttttg cgcaggcctc cagaaaaaac ggctcatatt
     4261 cggggatgat gtcaagaccg gcaatgcggg cccgcggatg aagacggcac ttgagcgcgc
     4321 cggtgaagga gccgaacgcg catcccagtt ccaaaacatt ccggcacgag gtcagatccg
     4381 ccccggccag ggccgtctgc cgaatatcat ccgggttggt ggaacagcgc cggataatcc
     4441 gttccatttc ccgatggttc agcacatcgc gaaaaacctc acggattttt ttgtcatcgg
     4501 aaggatcggc catgttttcc cctgcaatgc cggcatgcgg ggcgggtatt tgccgcctgg
     4561 ttgcgcgcgc ccggctagag ttcgatgtcc aggccgtcat aggccaggtc gattttaaga
     4621 ctggacgaac cgtcgaggat tttgagatag gcgcgggtat cctccagaaa ccggtccagc
     4681 gcgtcgtcgt caaaggtgtg ctcgttgtga aagaggcaca ggcgccggac accggcggcc
     4741 gccgccattt cgacgcccag cagattgctg gaatggcccc agttctcctt ggtgtccacg
     4801 gcgtccagga aacggtattg cgcatcgaag atcagcaggt ctgcatttga aaaaaaagag
     4861 aggaaccgct cgtactcctc cctgccgccg tccttgtgct ccgagtcggt ggagtagacg
     4921 acggtccggc cgtctttttc aaagcggtac ccgtaggagt ctcccggatg gttctgcctg
     4981 atgcaggcga ccttcattcc ggcaatgtcg tattcgcttt caggctcaag ggtgtggaag
     5041 cggatgtcgg ccttcatggc atgaaaaccg acggggaaac agggttcgct ctgctggcgg
     5101 ataaagattc gttcaagatc cgcatggccc ccgtagatat ggatgcggtg gcccggcagg
     5161 taggcgggcg tgaaaaaggg aaagccctga aggtggtccc agtgaggatg cgacatcagc
     5221 aggtggaaaa cgttcggcaa accgggctgc ccggcggctt tcgccttcat gtaggcgccg
     5281 ccgaaatccc gaagccccgt cccggcgtcg caaaggacat attcttccgc gccgcaaagt
     5341 tcgacgcatg ccgtgtttcc gccgtagctt gcccggacgg ggaaggggag ttcgttgtga
     5401 acgaaggctt cgatggcctc gtccgtctcc agagaatggg tgcgggaggc ccggattgcc
     5461 tttacgattt tctcctcgac ggccccggcg gtgatcgagg cgggcaaaga tcctcttgtc
     5521 ccccagaaat atacccgcat catccttcct cctcatggtg tccggtattt cttccatggt
     5581 ccgcggtgaa ggcattttgt catattgccg cgggcatcgc aacgaaaatt atccgtatcg
     5641 tccgttacga cacggacagc aggtatgacc gaaggttaac cccggcattg accaggttga
     5701 gctttttccg gatatgattc cgatgggttt ccaccgtttt taacgaagcg ttcagcgcgg
     5761 cggcaatttc gccggttttt ttcccttgtt taatcaggtc cataatctga atttccctgg
     5821 gcgtcaggtc cgcgtgagcg gcaagaatgt ttttcaaaaa aggggacaaa atctcccgga
     5881 gattgtattc cagcgtctgg agatcgtttt tgtagcgatc ctcgagattt gcgcccttga
     5941 gttttttcaa gcacggaagg atgaggtcat ggacgttggc ctgcaacttc tcttcgatgt
     6001 ctttctggtc ttcgtctcgc tggtgcatta aaacgcgcag cgcgatgttg gcttcctcaa
     6061 ggctttcggc aaagcgcttc agctcgtttt ccgctttctt ccggtcggtc acgtccatgc
     6121 cggcgcccac gatgcacaac cggtcttcat cggacaggcg ccttgcgttg agccggaaaa
     6181 ttctacgggt cccgtctttg aaaatcagct ccgcctcgga ggagagctcc ccggcgcgca
     6241 aaatttcttt gacgccctcc agcgcggcgt ttctgtggct ttgaggacaa aggtccgcaa
     6301 acagcatttc ccggatttct tcgtccgcat atccggtcac ggccctcaga ttgtcgttcc
     6361 agcggagaaa tttcccccgc tcgtccagaa caaagaacag gcccggcagg ctttccgcga
     6421 ccgtccggct gaaattccgc tcattgatca gagattcttc cgcccgcttg cgctgagtga
     6481 tgtcgtgcgt gacgcagacg atgcagatgg tctttccgtt tttcacaaca ggagtcgcgc
     6541 tggtttcgat gtgccgcagg gttccgtcct tggcgaagat gatatagtcc gacggctcaa
     6601 cggttttccc ctccagaatg gaagccagct tggcgctggc ctgcgcgtag cagccgggat
     6661 ccatcacctt cttgattttc gcaaacggtt tgccgacaaa ctccagcgga tgatagccca
     6721 gatttctttc gaccgaggga gatatgctca gtatccggag gtccgtgccg agtatgaaaa
     6781 tgacatccga cacctgttca aagctcagcc ggtatttctc ctcctgctcc cggagctcct
     6841 cgttggcctt ttccaggtcc gccattttct tttccagctt gccgaataga attttatcgt
     6901 gcttgcgaag aaattcaatt tcttctttcg ccgggccggg aatttttggt ttggccggtt
     6961 ttcttttttc cagcagttcg atcagcagac ggatcagaat atgcggctcc tggggtttga
     7021 gaatgaagtc gtctggatcc aaactcaggg ccagcgcccg ttcccgggat ctcgtgtatt
     7081 cggcggtgta aaatacaaaa gggatggacc ggagcgtttc gtcgagtttc cattcccggc
     7141 agagggtata gccgtccata accggcatca ggatgtccga gacaatgaga tccggagggt
     7201 tgaggcgggc catggccagg gcttctttgc cgttttccgc catggccgtt tccaggccgt
     7261 tggcgttgag cagggcttcc agcagaaaca ggctgcccgg ttcatcgtcc accatcagta
     7321 ttttcttttt catggccgcc tcgacgggaa ttctttcgcg gatgatccaa ggttatctaa
     7381 agcttttttc gggtggaggc aagataaaaa tctgaacaga taaaatccga acaagcgccc
     7441 ccgggcctgc cggaaaggct ttttaacgct ccgaacacgg gcgccgggct tgctttttgg
     7501 cttccgtcag caatatccat tgatgaattt tcagaaatat gccagattac cgcccatcat
     7561 catggaatca gcggttcggc gccgtaaaat cggtcggcgt acggcggtcc ggggcggcat
     7621 gacgcgggac cggcggaaaa gggcttttcg gcgcgccgac gccgctgaca tggagcgctg
     7681 atggacgatt gtcgcgtgca gctggccaat cttgtggact cccgggaccc caaagccgtt
     7741 cttgaggaaa ttcatgtgat tgccggtctg atggtgccgc ggtttgattt tgcgcctttc
     7801 gaccgggcct attcggacgt ggtccgtctc tttcgcggcg aatatccgtc ctatcgctcc
     7861 tgcaacgtcc aataccatga tctcggccat accctttcgg tcacacttgc cgtggcccgc
     7921 ctgatgcacg gagccgtgga agccggacaa tcgctaaccg aaaaagacct caacatgggt
     7981 ctcatcagcg gcctcatgca cgataccggc tatattcagg aaattgccga ccggcagggg
     8041 accggggcca agtacacgca ggttcacatc agccgcagca tctcgtttct tcaagcctat
     8101 taccgcggca atccggcgtt ccaaaacgat ctggattcgt ttcgggatat tttgctgtgc
     8161 acggggctca acacccgtgt ggacgccatc cggtttgccg gcgcgacggt tgagctgctg
     8221 gggaaaatcc tgggcacggc ggatctcctg gggcagatgg ccgatcgcta ctatctggaa
     8281 aagctgccgg aactctacga ggagttcgcg gaggccggca tcacgacctt ttccagccgg
     8341 ctggatcttt tggataagac gcaggggttt taccggatga cactgaagcg ttttgaagat
     8401 gaactgggaa acgtgcagcg tttttcccgc tgccacttca ggaagcgctg gggcattgat
     8461 gaagacctct atattcagag cattgaaaaa aacatggcgt atctggcccg ggtggtcagt
     8521 cttcaccgcg agaattacgc ggatcacctg cgcaggttcg ctcatacaaa agaacatctt
     8581 taatggtcat ggaggattaa aatgaaaagg attgtatgtc tgttgacgat actgttgctc
     8641 gcggccggga tttgcggggc tgccgcggcg gaagactcac tgcaaaacat caaaaaaaga
     8701 ggcgttttac gggccggcgt caaagacctg accccgagct tcgcgctggc cgacatcaag
     8761 acgggggaaa tcagcgggta tgacgtggat tttgtccgcg ccatcgcccg cagacttgac
     8821 gtccgccttg agatcactcc cttcagcggg gaggaaaatg agagaatttc cttcctgacg
     8881 gagggcaaag tcgatatcgt tgcggccacc atgaccatca cgagagaaag agaaaagctg
     8941 atcgatttca gccacccgta ttttgtgacg gcccagaaat ttctggtgca caaagacacc
     9001 gtgcagcggc tgagcgatct gaagaacaag caaatcggaa ccgtggatca gaccacttcg
     9061 gtggagaatc tccgcaaagc tctcccctcg gcgtccatcg tgctcttcac cggttatgtt
     9121 ccggcgtttc tgtcgctgca gaacaggcag attttcgccg tttcgacgga tgaggccatc
     9181 ctggccggca ttctggcaaa atccgacaaa gacgtatttg aaattccgaa ggtcagcatt
     9241 tccagagagc cctatggcct gggggtccgc aaggggcaga cttcattttt gcgttttgtg
     9301 aatcaggcgc ttctggagat ggagcacagc ggcgaagcca aaagaatctt tgacaagtgg
     9361 ttcgggccgg agagctccgc cccgctggag aggacgttca ggatcaccgc gaaataaaga
     9421 gtaaaaacgc cccgcggcgc aagaaagcgg gccggtgcga atcagcggct gttctccagt
     9481 gcggcgatct tggcgattct tcgttcgtga cggccgcctt cgaacggggt gctcaggaat
     9541 ttttcgatca tttcctcgac cggcacggcg tagcgcactc tgccgccgaa ggcgatgaag
     9601 ttggcgttat tgtgagccct ggccatttcg gcgctgaacg gatcgccgat aagggcgcag
     9661 cggatgccgg cgattttgtt ggcggctatc gaaacgccga tgcccgtgcc gcaaagcaga
     9721 atgccgaatt cgtagccgcc tttcaggaat tcccggcagg cggccgcggc cgtatccggg
     9781 taatcgacgg attcgctacc ccatgttcca agatcggtga cctcgaaatt cttcctttgc
     9841 aaaaaggcga tgatcctgtc cttcagcgca accgctccat gatcgccggc gatgacgatt
     9901 tttttcatct gcggactccc cctcctgacc cttgatgacg cttctttcta caagatgccg
     9961 gggaatgtca acaaaaaatc tttacatcag catcggattt gcgtagaaaa atgccatgcg
    10021 cgaatggctg aaccggtatt atcaatatct gctgatcgag aaaggggctg cggacaacac
    10081 gcttgcggcg tacgggcgtg acttgaaccg ctatctggcc tttctcgaac agagggggct
    10141 ttcgaatcct tggtcggcac ttccgcagac ggtggtggat tttctggtgc agatcaagtc
    10201 agaagggctt tcggccaatt cggcaaaccg gacgctggcc gccctgcgcg gcttttacaa
    10261 gtacctgctg agggaaaagg ttttggagga atcgccgctg gctcaaattg agctggccaa
    10321 agtctggatg cgtctccccg acacggtcag ttccgatgaa atgaaaaaga tcctgtccca
    10381 gccggacgat tcgaccccgc agggtttgcg ggaccgggcg atgctggagc tgctctacgc
    10441 gtccgggctt cgcgtgtcgg agctggccgg cctcaccatg aacagcatca actggcaggt
    10501 gggttttctg accgtcatgg ggaagggcgg caaggagcgg gtcgtgcccg tcggcaaaac
    10561 ggcctatgac tgcgtgcggc gatacgtcga tgaagcgagg ccgaagctta tccggtccaa
    10621 aacgacggac gttttatttc tgaatcgctt cggaacgtcc ctgacgcgcc agggactttg
    10681 gaaaatcatc gtccattacg cgcaaaaagc gggcttgaaa aaaaatgtgc acccgcatac
    10741 attccggcat tccttcgcct cccatctgct ggaggggggg gcggatttgc gcgcggtgca
    10801 ggtgatgctc ggtcattcgg atatttcgac gacgcagatt tacacgcatg taacccggga
    10861 acgcctgaaa gagattcacc ggaaatatca tccaagaggt tgaagatttg cgaaaaccgg
    10921 cagggcttaa aatcggattc ggcaaaaaag tctttgcggc ggcgggcatc acggcgctgg
    10981 ttttggccct tttgacggtg tttttcccca aaacctttca aacggccggc ttttatctcc
    11041 agagaggaat caacggtttt tcttattata ttctgggggc ctcgccgcat ttttatcatt
    11101 tggagatgga aaaaaacgcg agcgattttc gcgtcagagc cggagaaacc ctggacgtaa
    11161 cgtatcgcga tgaattcgtg gtcaaatccg tggccagtga tgactggagc ggaaaaagcc
    11221 tggagccggt tctggagggg cccggcggca aagccggcat catcggcgtg ccgtttcgcg
    11281 gaattgacct ggtggacaaa ctgctcgccg gcggcgtgat tcccgagggg acgcaggccg
    11341 ccggtgttta tcaaattgcg gtctattcca cgcgggacaa aatcggcgtg gtgcccctcc
    11401 gggtgatgat cacgccgcag gactggctga ggttcgcaaa gagccgcggc aatccgggcg
    11461 agcaggaggc gtacctgaag aaggcggctg ctcaaaaccg ggaagacgcc ggcgtgcgca
    11521 agatccttgc cggcatttat cttcagcaaa accggatcgg ggaggccgcg aagctgtatg
    11581 aagaaatttt gcgcatcagc cccggcgatc ccctcgcgat gaaagaactg gcccgctgca
    11641 ttgcacagct cggccgggag gaccgcgcca tcgatctttt gtcccgtctg acggaagtcc
    11701 agacggagga tgccgaagcc catgccatgc tcggagccgc ctacgggcgc aaaaccatct
    11761 gggacaaagc ggcggacagc tatttccggg ccgtccagat cgatccggac aaccatgcgg
    11821 tgcggctttt gctggcccaa gcctatgaga aatcccgccg gaccgacgcg gcggtggaac
    11881 agtaccgctt cgtcatccgc catgcacccg acgcagcccc ggcctggcgg gcgatgggcg
    11941 acatgcacct gaagagcggg cgatacgcgc aggcgattga gagctacacg cgcgtcctcg
    12001 accttcagcc gaaagacgcg gcggcgcatg ccaacctggc cacggcctac gccggtctgg
    12061 gcaaagcgag ggaggaaatg gagcacctgc agaaggccgc ggcgctgtcg cccgacgagc
    12121 cggtgatccg cttcaatctc gcggccgctt atgaaaaaag gggcaagacg cccgacgccg
    12181 tccgggaata cggccgggtt ttgaaaaaca agccggacga tgccgacgcg ctcgcgcgcc
    12241 tggcggatct gacctttcaa aacaaacagt atgagcaggc cgccggttat tacgaaaaac
    12301 tctcgcggat tcacccgacg cgcgcgtcga tattcgttag gctgggattt gcccgcgggg
    12361 aactgaagca gtatgccgca tcggccgaag attacgaaaa ggctctccgg ctgggggcaa
    12421 aagaccaggc cctgtattac aatctggcgt ttacctatac gaagctgggc agggaaaagg
    12481 atgccgtccg ctactacgaa aaaatatcgc cccagaataa aaaaacgctg agcattctgg
    12541 ccgatcatta cctgagagaa aaacagtacg cccaagccat ccgttattac cagagaattc
    12601 tgaaactgga gcccaaaaag gcgtcttcct gggccggcct ggggtatgcc tggcttgcct
    12661 ccggaaaccc ggataaagcg attgagcatt attcgcaggc tttgaagtac gaccgcgagg
    12721 atgacgagat ttacgccaac ctgggcatcg cctatgaaaa aaaagggctt tacccggaag
    12781 ccctgaaagc ttataggagc gcctacgaga tcaatccgga aactcgggtg gcgccgcgga
    12841 ttccgaggct gcgcattcaa cttttgcata aaaaggataa cagaggaagc gaaccatgaa
    12901 gcaagtgaag ctgaattccc acacgatcgg cggcaaggcc gggtttattt tgatagctgg
    12961 cccctgcgtt ctggaaaatg aagcatcggc gatggagatt gccgtgtatc tgaaagagct
    13021 gacggacagg ctggagattc cctttgtctt taaggcatcc tacgacaagg ccaaccggac
    13081 gtcggttgcc gcttaccgcg ggccgggcgc ccgcgcgggg cttgccatat tgaaaaacgt
    13141 ccgggaaaaa atcggcgtgc cggtcctttc cgacgtgcac cggtttgaag aaattgagga
    13201 agcggcggag gtgctggatg tgatgcaggt gcccgcgttt ctgtgccggc agacggattt
    13261 tgtcgtggag gtcgcgaaaa aggcgaagat cgtgaacgtg aagaaaggac agtttcttgc
    13321 gccctgggac gtggccaaca tcatcgggaa gattcagtcg gccggcaatg aaaatattct
    13381 gatcaccgaa cgcggcgcgt ccttcggata caacaacctg gttttcgatg tgcgcgcgct
    13441 tccgatcatg cgggcgatgg gatatccggt tatttttgac gccacgcatt cggtgcagtt
    13501 gccgggcggc atgggaacct cgtccggcgg ccagcgggat atggtgccct acctggcgcg
    13561 ggcggccgtc gcggcgggcg tggacggcat tttcatggaa gtccatccgg atcctgaaaa
    13621 agcgctgtgc gacggggcca attcgcttta tctgaattcg cttaaagaac tgctgacgac
    13681 gctgaagaag atcgatggaa taacgaaggg aataggccat gggtcatagg cgatgggcga
    13741 tagggaaaaa gctcatagct catagtgaag agcgatgagt ggcgagtaaa ttgttgggcg
    13801 ggtcttcaga cccgacgtga tgaacagtgg atggcggatg gtaaaaaaaa gcaacgagcg
    13861 gaaagcgatg agtgaagagc gaataatgaa aaagaagata cggggaaagc ttgaaaagat
    13921 aaagatgctg attctggatg tggacggggt gatgacggac ggcagaatca tcatggacag
    13981 cgacggacgg gagataaaaa acttttatgt ccgcgacggc cacgggctgg tcatgcttca
    14041 gcgccacggc attcgggtcg cgatcctgac cggacggaca tcttcggtgg tcgatcatcg
    14101 ggcgcgcgag ttgaaaattg cgcaggtata tcagggggcg ctgaacaaga aggaagttta
    14161 tgcaaaaatt cttgagaaaa acggcctggc gccggaagaa accgcttacc tgggagacga
    14221 tattgtggat attcccgttc tgaagatggc cggattttcc gccgcggtgg ccgatgcggt
    14281 ggatgaggtg aaaaagcatg tcgattatgt cacgctttgc cgcggcggga agggagcggt
    14341 gagggagatc tgcgagatgc tgcttgaggc gctgggcttc tggccggaag ttgccagacg
    14401 ttatgacttt gacggacccg aatagcttta cagggaagag agcgagtgat atatcaaacc
    14461 catgggtttt caaagaaaag taaaaattgc agcgattacg ctgatgattc ttgtacttgc
    14521 gggcgcggtc ctgtttgccc ttctgcgggc gaccggcggc aaaaaagaga cgattgtcaa
    14581 gatcctgcct gacgaagcgg atgtccgcat cgcggatttc ttttatacgg aagtcggcaa
    14641 ggacaatctc cgctgggagg tgaaggcgaa aaccgcccag tatcagaaga agaggaatct
    14701 ggccctgttt gatcaggttc agatgaaact gatcacgcag aaaggcagga cctttgtgat
    14761 gacgggcgac cagggcgaga tgcggaccga cagcaaagac gtcgaaatcc ggggacatgt
    14821 cgtggcgacg gccgatacgg gagaaaaatt ctccacggag gttctgcgct acagcgacgc
    14881 tcggaaacaa atttataccg acgcctccgt ccggatggaa acgagccgca tgaaaattca
    14941 gggagtcggc ctttccattc tgatggataa aggggagctt gcgctttcct ccggcgtcaa
    15001 agcgaaaatt tactgatggg accatgttga tgaaaaaaat agtcctgatc acggcgggga
    15061 ttctggtcgc cctgaaccta caggcctggg cgcaaaccgg tttgcttcag aaaaacacgc
    15121 ttcggaaaaa tgagccgatt cagattgttt ccgataaaat ggaagctttt caggccaccc
    15181 gaatggtggt gttttccggc aatgccgtgg cgcagcaggg ggatgtccgg atccaggcgg
    15241 accgcctgtc gatttactac aagaactccg ccgcccagag cgaaaaagtc ggaccggacg
    15301 cggagcagac gggcgacctt gaaaaaatcg tcgccagcgg ccacgtggtg atcacgcaaa
    15361 aagatgtgct ggccacgggc aatgaagcgg tgtattatca ggacacggcc cggttcgtca
    15421 tgacgggaaa ccccgtcctg cggcaggaaa aaagcgtgat taagggctgc agggtcatca
    15481 ttcaggtcga tgagaaccgc gggaaagtgg agcagtgcgc agacgaaaat tcgggaaggg
    15541 tcacggccat catccacccc cggaagaaaa aataatcctg cgtgacgggc tcccgagagg
    15601 gaaaatgagt catttatcgg ccaggggact ggttaaaatt tacaacaaaa gaaaggtggt
    15661 caaccacatt gacctgacga tttccccggg ggaagtggtc gggcttctgg ggccgaacgg
    15721 cgccggaaaa accacgacgt tctacatgat ggtcggactg gtcaagccgg acggcggtca
    15781 aatctttctg gacgacgagg agatcggcgc gtatcccatg taccgccggg cgcgaaaggg
    15841 attgaattac ctgccccagg agccgtcgat tttccgaaag ctcaccgtcg aggaaaatat
    15901 tctggcgatc ctggagacgc ttcagatcag cgaagaggag cgccggcgaa ggctcgagga
    15961 gcttctgggc gagctggacc tgacaacgct cgcgaaaaac cgcgcgtatt ccctgtccgg
    16021 cggcgaaaga agaagggtgg aaattacccg cgcgctggtg acgtctccga aatacatttt
    16081 gctggatgag cctttcgcgg gcatcgatcc gctggcggtg gccgacatcc agaatattat
    16141 cgagaagttg aaaagcaagg gaatcggaat catcatatcc gatcacaatg tccgggagac
    16201 gctggcctgc tgcgaccggg cgtacatcat caatgagggc gcgattctgg tggaaggccg
    16261 gccggatgta atcgcccgga gcgaactggc caaaaaattt tatttcggcg aggatttcag
    16321 tctgtagcgc cggacggata cgcgatgttc agccatggct tttgaattac ggcaaaactt
    16381 aaaactttcc cagcaactga tcatgacgcc gcagcttcag caggcgatca agttgctgat
    16441 cctttcgcgc caggagctgg tggacacgat caaccaggag atggaggaaa atcccctgct
    16501 ggaagaagtg gcgccggacg atgaaagcgc cgaggaactc cacgccgaag tcgcggagga
    16561 tgtccagtcc gttgaaaaag aggaaagcaa gattcccgag cacacggccg agctgaccgg
    16621 cgaaggggac gggcgggaag aattcgactg gaacaattat ctggaagatt acggtccggt
    16681 gggcgtttcc tatgaccggg ccgaaggaga cgccccgacg ctggacaacg tcgtgacgga
    16741 aagcccctcg ctgaccgaac atctcatgtg gcagatgaag ctctcgccct tcaacgggga
    16801 agaggtgcgc gtcggggagc agatcatcgg caatcttgat tccaacggtt atcttcgcgc
    16861 caccgtggag gaaatcgccc gtctggaaaa cgtcgccgaa gagttcgtgg aaaaagtcct
    16921 gaaaaaggtg caggaatttg atcccgcggg cgtcgccgcc cgggatcttc aggaatgcct
    16981 cctgattcag gccggcatgt tcgcccggga aaacccgctg attgagccca ttatccggga
    17041 gcacctcaag gagctggagc tgaaaaatta cctgcagatc gcccgcaggc tcaaagttcc
    17101 cctgaaggaa gtggaaacag ccgttttgct gatcagcaaa atggacccca agccgggcgc
    17161 cgtctattcg gaagagaagg cccaatccat cattccggac gtgtatgtgg tcaaatcggg
    17221 ggatgactat aaaattattc tcaacgatga cgggctgccg cgtttgcgga tcagcaattt
    17281 ttaccgtgaa atcatgggcg gcgtcggcgg accggactcc gcggaaaagg aaaacggccg
    17341 gaaatacatt cgggaaaagg tgcagtcggc cacctggctg atcaaaagca ttcagcagcg
    17401 gcagaaaacc atttaccgtg ttgccgaaag catcgtgaag caccagaagg aattttttga
    17461 caaaggcatc gactatttga agcccctggt gctgcggaac atcgccgacg atgtggaaat
    17521 gcacgagtcg acgatcagcc gggtggtgac gaacaaatac atgcattctc accgcggcat
    17581 ctttgaaatg aaatattttt tcggcagctc cattcagaga acctccggtg agaatatcgc
    17641 ctccaaaagc gtaaaggagg agattcggca gatcatcggc aaagaagacc agaaaaaacc
    17701 ctacagcgat tgtgaaatcg tgcggctgct gaaggcccgg aatatcgaca tcgcgaggag
    17761 aactgtggca aaatatcgcg aaatgactgg tattttgccg tcgtccaagc ggaaaaaata
    17821 tttttgacgc aggcgttttg cagcgggaaa ccatcaaggc aaaaagtaaa attccggttg
    17881 acatttggtt tcgtcgccac tatacgacta acctttgttg ccggccgtga aggcgtgttt
    17941 tgaatgtttt taaacggcct aaactcaaga gggaggaaaa aggatgaata tttcggtgac
    18001 tttcagaacg ggcgaagggg agaattggca aaaggaatac gcggaggaaa aaattgccag
    18061 actgaaaaaa tatctcgatg tgcctgccga cgtccatatg atcctttcca cggaaaaatt
    18121 ccgcaacatg gtggaaatta atctgaccgc caacggatgg aatgtaaacg cgaaagaaga
    18181 agccaaggac atgcgcctgg ccgtggaccg ctgcatcgtg aaaattgaaa accagctcaa
    18241 aaaacaaaaa gaaaaaagca ggcagcacaa gcccaaaagc atccgtcacg ccggcgacaa
    18301 agccctggaa ggcgcggagg aggaagatgc cgcctccaaa gtggtcgaaa cccgcaaaat
    18361 cattttaaaa cccatgtctc tggaggaggc ggttctcgaa ctggagagcg gcaaggcgcg
    18421 tttcctgatc tatcgggaca cggcttcgga gaaagtgagc ctggtgcacc gcctggatga
    18481 cggccagctc gcgcatattg aaacaaacag ctagttgcca aaagccgggt ccgcggcatg
    18541 aaaatgcgcc ggcccgtcgg gatcatggat ctatgagacg tcaggacagc gtcccggatt
    18601 tcaaatacag gtgtggcgca tgcgactgga tcaaatattt aaaatggaat ttctcagcga
    18661 aaatctcctg tccgacacca aagaaggagt gcttgccgag ctgatcagtc ttctgatcgg
    18721 aagcggcttg aagattgacc gggcaaaggc gcttgatgtg ctgcagcagc gggaaaagct
    18781 gggctcgacg ggcatcggcg acggcgtggc cattccgcac ggaaaggttt ccgaactgca
    18841 ggacatggtc attgccttcg ggagaagccg ccggggcgtt gcctttgacg ccattgatgg
    18901 aaagccggtc cagctttttt ttctgctgct ggcgccggag cagtccacgg ggcagcacct
    18961 gaaggctctg gccagaattt ccaagatgtt gaaaaccccc aactttcgga aaacgcttgt
    19021 cgatgcgaaa aacagagccg atttgtacaa agccatcgtt gagcaggatg cgacctgtcc
    19081 cctctgaacg ggatgtgcct catccggagg cttccgcacg cgaatggttt cgggactatc
    19141 tgccgtatct gggcattcga acaatttccg gcccgcgcaa tcacaaaaat ccttttcccg
    19201 ccggcgcggt ccagtgtctg cggagtccga ccgcattccc ccggcgggaa gccgcgattc
    19261 ttctttttcc gcccgagacg gcaaataaac ttctggcgct ttccgggccg tcccgcgagg
    19321 aatatttttc ccgcatggcc gggcgccgtc cggttttgct gattttttcg caaagcccgt
    19381 ccctgccggc cgtgatccgc aatcaactgc ggaaccactg tctttgggcg gccgcttccc
    19441 cgcttcacga gaatctgctg gccagccgca tccgttcaat cctcctggag aaaattcatc
    19501 aatgcatggc gatgcacggc gtggcactgg cttgcgggga ccggggcgtg ctgcttaccg
    19561 gacccagcgg catcggcaaa accacggccg cgctggaaac cgcccgcgaa ggctgtttct
    19621 ggatcgccga cgatctggcc gtgattaaaa atgttcgggg acggctgatg atttccggcc
    19681 acccgagaat tcaaaaatat gtccatgcgg gggagaaggg aatcgagttt gcaaatcggg
    19741 tgctgccggc gcggcggaca aaacaaaggg cggagctttt cggcattgtc gatgtctgcc
    19801 ggagtgccgg gaagaaaatc tcccggccca tagagatccg gattttggaa aagcgcctgc
    19861 cgctcgttcg ggtgtgcatt tcccggactg gatatttgcg tcaaaatctg ttaaccagag
    19921 ccatgcaaaa actgaacgag gtcggataat tgaaaaatct ccaggtggtg atcattacgg
    19981 gattgtccgg ttccggcaaa agcaccgcgc tccgggcgct ggaagacatc ggcttttttt
    20041 gcgtggacaa ccttccggtt gtgctgctgc cgaagtttct ttccatcacg ctgcagtctt
    20101 ctccgaagat caggcaggtg gcgatggtta tggatttgcg gcagaaaagc tttctggaga
    20161 agtaccagcg catctttgaa cgcctgaagg aaaagggcta ccggatcaat attctgtttc
    20221 tggaagccgg ggaagagtct ctgctgcgcc gcttcagtga aacccggcgc agccatccgc
    20281 tctccgaaaa gcgctccatc atggaaggca ttcaggaaga agtgaaaaag ctttccaccc
    20341 tgcggcagat ggccgacacc gtcatcgaca ccaccacctc caacgtccac cagctcaagg
    20401 acaccgtgca aaagcagttt ctgccttctt ccacccggaa aaaaatgatg atcagcgtca
    20461 tgtctttcgg ataccgctac ggcctgccgg cggacgcgga cctggcgatc gacgtgcggt
    20521 ttctgccaaa tccctattac gtggaaaagc tgaaaaacct aaacggtcat caccgcgatg
    20581 tggagaagta cgttttgaac aacgaggaat gcaaaacctt catgaacaaa gcggccgaca
    20641 tgctgaactt catcattccg ctgtatgaaa aagaaggaaa ggtgcggctg aatgtcgcgt
    20701 tcggctgcac gggcggcaag catcggtccg tcgtcatggc caatcagttc agcctttttt
    20761 ttcaaaagca gaagttcatc gtccacacca cgcatcgcga catcaataag agttaggcaa
    20821 gcgcgcttca cgccgcctgt ggcggcgact gatgagggag tccgttcggg gaacttgctc
    20881 agaagcgtgc aggcaacggg cgacaggcta taggaatccg ttttatcccg cctatcaccg
    20941 attgcccctc gcccatagcc ttatagattc atctttttca tcacgttgtc ttcaatccag
    21001 tgcgggtccc accattcctg cgggttgaca aactcgccgc ccaccagaat gctgaaatgc
    21061 agatgatcgc cgccggccag tccgctcagt ccggagacgc cgattttttc ttcttttttg
    21121 acgcttttgc cgacggttgt ttcaatggcg gaaagatgtc cgtacaggct gaacaggccc
    21181 aacccgtgat cgatgatcac ggtgttgccg taaatgccca gcgcgcccgc gaagacaatg
    21241 attccgttgg cggcggcttc gatgggagcg tgtgccgtcg aggcgagatc cacaccaagg
    21301 tgcaccgagt tgccgaaggg tttgccgtcc accaggtagg tgcgctgatc gccgaacagg
    21361 gccatggggg cggcgtcgcg catccggtga aacgcgcctt cccataattt tttattgatg
    21421 gacttgcggc agacggattg aatggttttg gcgttctctt cccggagagt tgtgttgaca
    21481 tagccaaaga cttcaacggg cgtcttcccc tgcagctgcg gaatcgacgc ctggaattcg
    21541 ggcatgattt tctggagaaa ggtttcgctc aaattcactt tgtccgcgcg gaatttcttc
    21601 ggcttgatcg taaagggcat ggaggttcga gcttcgttgc cggcgttgtc ccgggcgaaa
    21661 atgacgattt tggtcgccgc ggaagcgccg gttccgaggg cgaagaaggc caccgacgtg
    21721 ggcttgccgc cgatgacgat gttgtatccc gggtaaaacc ggtcgtccac gaagacgccg
    21781 gtcagcgcgg ccggtttgga cgtccggtac gggatgaagc cggcgcctcc ctggttgatg
    21841 tagttgacgg ggctcattac gtaaacctga ggcggcatca tgtcaatccg gacctgctcg
    21901 gagatgacgg tttcattttt aaagagggag gcatccccgg ccgtgatgcg gacgacggcc
    21961 ggtccgttgt tgagctggag caagaggggc tccaccgtaa ggcgaagaac cttttgcctg
    22021 gtgctccggg acggaaaaga ttcctgtgcc agaagacgcg gcttgttgtc ctgaacaatt
    22081 tccaccttca ggcccgacag gccgcttcgc tcgtcggaaa aggcgatgct gatgtccttt
    22141 tttttgccga tggccgtgat gtccgaatcc agccgaatcg cgggtttgtc ccgatccagc
    22201 cagccggcaa aataaagcca gcccaatgcc cccgccgcca gaacgatgac gaccgccgcc
    22261 gcaataatcc ccgtcttttt catttccgaa gctccttgat taaatcctga tattgaattg
    22321 gcggttttca taatggcttt tgccgcggtt tgcaaggaaa atatactttt tgcgcgggcg
    22381 catggaaaga ctttatacct tgatcttgct gggaaacgct gttatattcg ccgaaaaaac
    22441 agcgaagtga aagacaaatg ctgaaaaata agaaaattgt tctgggcatc accggcggta
    22501 ttgccgccta caaatcagcg gagctgaccc gcgctttcat caaagccggc gccgaggtca
    22561 aagtcatcat gacccaaagc gcgacggagt tcatcgcgcc gctgacgctt cgaacccttt
    22621 cccggaaccc tgtctatgcc gacatgtttg aggacgcgga gccatacgac atggcccata
    22681 tcgcgcttgc ggaatgggcg gacgcgttcg tcgttgcgcc ggccacggga aacgtgatcg
    22741 gcaaaatcgc ctccggcatc gccgacgacc ttctgaccac aaccctgatg gctgaaaaca
    22801 ggcccacgct gatctgcccg gccatgaacg acaaaatgct ggccaatgcc ttcgtgcagg
    22861 agaaccttcg gaaactcgcc ggggccggct atcgcgtgat gcaaagcggc gtgggcgagc
    22921 tggcctgcaa cgcagcgggc gccggacgcc tgccggagat tccggagatt ctggacgaga
    22981 tggaagcgct tttaaccgtc caggattttg ccggagaaaa agtattggtg acggccgggc
    23041 cgacgcagga gccgctggat ccggtgaggt acatcaccaa tcattcatcg ggcaaaatgg
    23101 gctacgcgct ggcgaaggcc gctcatcaga ggggcgcgca ggtgacgctg atcagcgggc
    23161 cgaccaggat cgttcccccg aaagttgaaa cggtcgtttt tgtgcgcacc gcgcaggaaa
    23221 tgcaccgggc cgtcatggac gcctggacca aagcgtcggt cattatcaaa gccgccgcgg
    23281 tggccgatta ccggccccgg gttgcggccg gcgagaaaat caagaaagac cgggggctgt
    23341 ccggcatcga gcttgtgcgc aatcccgata tcatcgccga catcggcgcg aaaaagaaga
    23401 aaggcgtcct ggtgggattt gccatggaaa ccgaggacct gctggacaac gcgcgccgga
    23461 aaatgatccg gaaaaacatg gacatgatcg tcgccaacaa tctgcgcgac gatggcgccg
    23521 gttttgaagg cgacaccaac atcatcacga ttcttgaccg gctcggtcat accgtcgctc
    23581 tggataaaat gaccaaacag gatgcggcgc accgaatcct ggatcatatc cgctctttta
    23641 aatccaagaa aggacccgcc ggaaaaaaat gacgcaatcc tttccgccat cttcatccga
    23701 cgagattcgg aatgaactgc tgtccgtggt gaaatccgtc agaggacagg tgctggacgc
    23761 agagcgcctg tatgtcgccc tggactgcaa aagacctcag ccggcccgga aaaacacgcc
    23821 ggacggcagt gatcgcggcg aggcgcttca ctccctttcc ctggaagccg cgaactgccg
    23881 gcagtgtccg ctcgcgcaaa cgagaaaaaa tgtcgtattt ggcgccggga gtttatcggc
    23941 tcaggtggtt tttgtgggcg aagcgccggg cgcggacgaa gatgaacagg gccagccttt
    24001 tgtcggcaag gcgggacaat tgctgacgga cattattgtg aaaggaatga aactgacgcg
    24061 caaagacgtt tatatctgca atatcctcaa atgccgcccg ccggggaacc gcaacccgct
    24121 gccggaggaa atccgtctgt gcgagccgtt tttaaaaaaa caattgcagt tgattcggcc
    24181 ccgtgtgatt tgcgctctgg gaaaatttgc ggcgcaggta cttttgaaaa cggaaacgcc
    24241 gatttccgct ctgcgcgggc gctttcaatt gtacgaagag atcaaactca tgccgaccta
    24301 tcatccggcc tatcttttga ggaacccgtc ggccaaaaag caggtctggg aagatgttca
    24361 gctgatcatg aaagaaatcg ggcttggcgg accgtcatga ggaaagaggc gtcaccatgt
    24421 tgaaaaaaac gggaatcctt ttcatgattc tcctgtggat cgcgtcggcg ggcgttgtcc
    24481 ccgccgagga agccaggcca ctgtttcatg tgatcgagct gggcggcgta attacatccc
    24541 catcggcaaa atacgtcgcc cgaagcctgg aagaagcgac ggccgcgaag gcggaaggac
    24601 tgatcattct gctggatacg cccggaggcc tggacaccgc gatgcgggac atcgccaaaa
    24661 gcattctcaa cgcgccgctg cccgtaatcg tttacgtcta tccgtccggc gcgcgcgccg
    24721 cgtcggcggg cgtgattatc gcgcaggccg cgcatgtcgc cgccatggcg cccggcacca
    24781 acatcggcgc cgcccatccc gtggccatcg gcctgggagg cagcggcgag atggacaaga
    24841 ccatgtctca aaaggtggaa aacgacgcgg cggcctatgc ccgcagcatc gccaaaacgc
    24901 gcgggcgcag cgaggaatgg gtggaaaaag cggttcgcaa aagcgagtcc atcaccgccg
    24961 acgaagccct gaagctgaat gtcattgata tcgtggccgt ggacatcgga aagctgctgg
    25021 ccgccattga caagagaacc gtgaccgtcg ccggggccaa aaagaaaata tccaccgccc
    25081 atgcggtttt ggacacaaaa aagatgggca cacggcaggg gattctggcg gcgatctcca
    25141 atccaaatat tgcgtatctg cttttgctga tcggtctggc ggggctgtac tttgaatttt
    25201 ccacgccggg cgcgatcctg ccgggcgtca tcggcggcat ttccctgctg ctggcgttct
    25261 ttgccctgtc cacgctgccg gtgagttaca cggggattct gctgattctg ttcagcgtca
    25321 ttttgtttat tgcggagatt aaaatcatga gccacggcat gctgacggtg ggaggagtca
    25381 tttcgcttgc gctgggatcc tttttgctgt ttgattcgcc ggagccggcg ctgagaattt
    25441 ccctgcaggt tctgctgccg gccgtgctgg tggcgtccgg attcttcatc gtgatcatcg
    25501 ggctggccgt caaggcgcag atgaggaagc attattccgg gacggaggcc atgatcgacg
    25561 gccgggggga agccgcgacg gacatccacg gcgacggcaa ggtttttttc cagggagaat
    25621 actggacggc gacgagcgat cgggaaattc ccaagggcgc ccccgtcaga atcatcaagg
    25681 tagaaggcct gaagcttgtg gttgaagaaa taaaagaagg caaataaatt atcgatataa
    25741 ggaggaagga ttatgttggg ttctttaccg ctgcccctgt tggtggtgat cgtgctggtg
    25801 gttatgtttc tggcttccgc gatcaaaatt ctcaatgaat atgaacgggc ggtccttttc
    25861 cggctgggca ggcttcgcga catcaagggg ccggggctga tcgttatcat tcccggcatt
    25921 gataaaatcg tccggatcga catgcgcacc attacgatgg acgtcccgcc gcaggatgtc
    25981 attaccaagg acaacgtttc catcaaggtt aacgcggtgg tttatttccg ggtgctggac
    26041 gccaacgccg ccgtcgtgaa tgtggagaat tatctttttg ccacgtccca gcttgcgcag
    26101 acgacgctcc gaagtgtttg cggccaggtg gagctggacg aaattctgac agagcgcgaa
    26161 aagatcaacc tggaaattca ggaaattctg gatcgaagca cggacccctg gggaatcaag
    26221 gtgtcgctcg tggaagtgaa gcatattgat ctgcccgaag aaatgaagcg cgcaatggcc
    26281 aagcaggccg aagccgaacg cgaaagacgg gcgaaggtca tcaatgcgga aggagagttc
    26341 caggccgcgc agaagctgat tgaagcggcc gccctgatgg gaacgcagcc catgtcgctg
    26401 cagcttcggt atttgcagac cctgaatcag attgccgcgg aaaataattc aacgacggtg
    26461 tttccgatcc cgatcgattt gttcaagcct ttcctgaatc tggcgggaaa atcgtaatcg
    26521 cgcgcagggg ggagccatcc cccccccgct caaaaataca ttttagagaa ggagaaggat
    26581 atgcctaacg aaaagagaaa cattcttacc ggcggactta ttttaatcac cctgggggtt
    26641 ttgatcttta tcagcaaaac cacttcctac agtttcggtc aaacctggcc gattctgctc
    26701 atcgttatcg gcatcaccac gctgttttac agtattcgtg ccgtcgaagg ctggtttgtc
    26761 gccacggtcg gcgtcatttt tctcatcatc gaactgtacg ggtttgattt atacaagtac
    26821 tcccagtata ttctgccggt aatcctgatc gtgctgggtg tctttgttct gctgaagcgc
    26881 cgcaagcgat aaaaaatcgg ctgcggaaaa aatacttgcc atgctctttc agcaaaaaaa
    26941 ggccttggtc ctcagcgatt ttgacggcac gatttcccgg gtggatgttg gagacggcgt
    27001 tttgagccgg tttgcctcag aaagccggga agccattgac agcgcatata tcagaggcgg
    27061 tatgggatcg cgggaggcgt acggaaaaat tgcgccgttg gtccgggtcg atgccggacg
    27121 tctgcgcgct tatgttttga aatgctccgc catcgatcct tatttttccc gtttttatcg
    27181 tctcgccgca aaaaaggggg tggatgtcaa aatcgtttcc gacgggcttg atttttacat
    27241 ccgggcgatt ctggacaagc accggctggg ggagattgaa ttttattcca ataccgtcgc
    27301 tttcggcggg gaggatacgc tgacctttga ctttccacag gccaatgccc tgtgcggccg
    27361 ctgcgggacg tgcaagagta aaattctcca tgaccaccgc ctgatgtatg aaaaaatcat
    27421 ctatgtcggc gacggccatt ccgatatctg cccgtcccaa tacgcggacc tgatttttgc
    27481 caagggcgag cttttgaaga agtgcaggcg ggaaagcgcc gttccctatc gcgcgtttca
    27541 tgattttcgc gaggtttcgg agtatctgaa gaaaaacgtt tagccaagcg gcgcgccttt
    27601 cggggagccg cccgggagaa gagatcatga gcatttcaaa gaaaatcaag gcgtccatgt
    27661 cgcagtcgtc attgatccgc aaaatgtttg aagaagggaa ccttctgaaa agcaggcacg
    27721 gacacgacaa ggtctatgat ttcagtctgg gcaatcccaa cgtggaccat ccgcgcgaat
    27781 tcaagaagga gctgattcgc gtcgcccgcc aggaaatcat cctcaagcac ggctacacac
    27841 ccaacgccgg ctacccggaa acccggcggg cagtcgccca aacgatcagc cggtccatcg
    27901 gcctgaaaat ggatgccgat cacattgtca tgtcctgcgg ggccggcggc gcgctcaatg
    27961 tgattctgaa gtcgattctg gatcccggcg acgaagtcct gatcctcaag ccgtttttcg
    28021 tcgagtatcc cttttatatc gaaaactacg gcggggtggt aaagatcgcg gacacacgcc
    28081 ccgatttttc tccggatctg gaagcgattg cccggatgat cacccgacgg accaaggcgc
    28141 tcattctcaa ctcgcccaac aatccgaccg gccgggtgta cagcaaaacg aatattgccg
    28201 gcctggcccg gctgctgaga gaaaaggaga aaaaatacag caaggccatc tatctccttt
    28261 ccgacgaggc atacgcgcaa attctttacg atggcattca actgccttcc gttctgacgg
    28321 cttatgccga cagcatcgtc gcgtattcct actccaaaac cctgtcgctg ccgggcgaaa
    28381 gaatcggcta catcgcagta aatccgaaaa ttcacgaggc ggacgacctg atccgggcgc
    28441 tgatcctgtg cacgcggatc atgggatttg tgaacgcccc ggccctgatg cagcgcatcg
    28501 tggccaatct tcagcacatc accgtggatg taaagattta ccagaagaaa agggatttgc
    28561 tctgcggcgg cctggcccgc gcgggatacc aattcatcaa accgcagggt gctttttatc
    28621 tgtttgtgaa aagtccggtt cccgacgatg tggcgttcgc gcagaggctc gtgcagaaaa
    28681 atattctggt ggttcccgga agcggattcg gaacgccggg ctatttccgc atcgcttttt
    28741 gcgtcgatga cgcgaccatt cggaattcca tgaaaggctt cgcagcggcc attcgggatt
    28801 gccggggctg aacccttgca tttggcactc atggcgccga ccaacgtagg gcgggtcgct
    28861 tgtgaccttc aggttatcat acccgccgaa ccgacatgca aggtccattt ggcgcaccta
    28921 aaggtgcgcc ctacataaaa aactgtcaaa agacggaagc tgttcctcgt gtttttgtcg
    28981 agcgctctga ttacagcata tttccgattt cctgaatcag ttgattcagg cgtttgggtg
    29041 aaacagggta gggggttttc agctcctggg caaaaagaga caccttgtat tcttcgatca
    29101 tccagcgcag ttcatccgtc ttgtttcgct tttcctccga tgcgtcggtg tccattgcgc
    29161 ctcggatttc ctgaagttta acggcgtaag tcgccgcctg gcgcaatttc gattccgtgg
    29221 acgccaggtt caggcttccc cgttcggccc gcagaaagag agctttcagg tagcggggca
    29281 gatggcgcag acggtcaaaa tcgcagtgca gaatgaaatc ttcggggacc aggacgtgtg
    29341 cttccgccag ggtcgcctgc agaaactcgg ccaagggcgg atgcggccgg tttttgaata
    29401 tcagtttttg caggccgtcg tgagtttcgg caaagcttcg aagaaccggg gcgatggccg
    29461 cgacgacttc ctgaccgtaa gaaaggatct tcggccggac ctcatcggca tgcttcataa
    29521 actctttcgg gctgcgccag gagcgccgga aaagatctct tttcacccgc tccagaacgg
    29581 atcgttcgac ctgccggggg ctgcccagca gggacgcctg ctctttgagt tcaccgccaa
    29641 gggagatgtt ttttttcagt tgcttgatcc tgtcctcgaa atggatggca tagagagcgg
    29701 ctacaccggc agcgtggacg gccgcggctt cccgggggtc ggcaaaaatc cgcagatgaa
    29761 cggctcctcc ctgatcctga agcgccggat aggctaagcc ggtcacgcca tgctttccgg
    29821 caatctcaac attctcgggc aggtctccga aatcccagct tgtaaggttc tccttttccc
    29881 attgcctccg gcaatcctcc aaagaggtgc gccggatctc ttcggtcagc tcccgttgca
    29941 attctccaat gtcgcggctg cttcggattt cacttccccg gtcgtcgatg acgctgtagc
    30001 ggatctgcaa atgcggcggc agtttttccg gcgcccagaa gccggccggc accgtcaaat
    30061 gatattcatc gaataaaaac ctgcttaacg ccaaaggcag ggcctggtct tcaccggcgg
    30121 tctgccggtt gaaccgtccg gcaacctcaa tcaggggagg aagattccgg cggatatttt
    30181 tgggcaggga ttttagccaa tgcagggcct tctcttcccg cagggacggc aaatgctttt
    30241 cgatgtttac cggggaggct ccggcgatga ggcccagcgg catgcggacg gtcacgccgt
    30301 cgcccgcgcg ccccggctca aacttgtagc ggcagggcaa gacggcattg cccatccgga
    30361 tctgatccgg ataccggttc aattcatcga ggtcgggtcc ggcggcaatc aggtcttcct
    30421 cccgaaagcg cagaaaagca tcgctccctc tgtctttgat gagtttctgc agggaacgga
    30481 tatcggaaag aagcggcaga cgctgttcat aaaggccggc gatggtttct tcatcggcgg
    30541 cgatgccgcg tttgcgcagc ttgtcttcca tcgttttgac tttttcccgc aacgacaggt
    30601 tgtgctctaa aaagggaagt ttgcgcccgg tctctcccgt gaccagcgct tcacgaatga
    30661 agattctcca tgcttcctcc gggttgatcc gttcataggc gacgcttctt tgctccacga
    30721 tggtcaatcc gaataaaaca actttttcca gggcaaccac ctgcccgcgg cttttttccc
    30781 agtgcgccgc ccagtgctgg cagcggcagt gctccccgcc cagctcttcg agccactcgc
    30841 tttgaatgtt ggccgcgttg cgggcaaaaa ccctggaggt caggctgatt tccgcggcga
    30901 cgatccaggg cccgccccgg ttgaaaagac ccgaacccgg atacagcatc acctcgcggc
    30961 ttttggcggc cgtgtaaaaa tttttttctt tcttttgcgc gatgttggaa agatagccgc
    31021 tcaggatcga gcggtggatt ttgtcggaaa cctcctggtc gatttcggct ctgacgggtt
    31081 ttcgccgcga tatcgcgccc gcttttccgc cgagaatgtc cagaatctgc gagtaaacgt
    31141 ctctccattc ctccatccgc cggtaagata gaaaatgatc gcggcagaat ttgcgccgct
    31201 gtccctgcgg ctgcatttgc tcaaaatagt ccgaataccg gttccagatc tgcaggtagg
    31261 cgaggaagtc cgattccgga tgcgcaaatt gcgcgtgcgc ggccgccgcc tgatctatct
    31321 tgtcgtaggg cctttcgcgg gggtcctgga tgctcagcgc cgcggcgatc acggcgattt
//
LOCUS       DAOPAF010000014        30418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1647_length_30418_cov_46.288311, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000014 DAOPAF010000000
VERSION     DAOPAF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 30418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30418
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1647_length_30418_cov_46.288311"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLQ77_02785"
     CDS             <1..343
                     /locus_tag="PLQ77_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09639.1"
                     /translation="AIPYRDEYIIAVGSSFEKYLASLDKLAGLDVRMICADHYGCVEG
                     VEAREYIGQTRAAALQMMDRMREALAQEGSVEAAARQLIRLHYERRPDYFVHPDILLR
                     TYTQMLKQLTG"
     gene            complement(348..1166)
                     /gene="panB"
                     /locus_tag="PLQ77_02790"
     CDS             complement(348..1166)
                     /gene="panB"
                     /locus_tag="PLQ77_02790"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPL09640.1"
                     /translation="MSTQTKISRKTILDIKKMKTQGEKITMLTAYDFGMASILDESDI
                     DILLVGDSLGNVVLGYDTTLPVTMEDMIHHTRAVARGSVKALIVADMPFLSYQVSQEA
                     ALANAGRFLKEGNAQAVKLEGGREHAEIVRKMTLSGIPVMAHLGLTPQSVHQLGGYKV
                     QGKKEDAAEKMMKDAVILEEAGAFSVVLECVPEKLAAEITRILSIPTIGIGAGVSCDG
                     QVLVVNDMLGMNDRMTPKFVKRYAHLSQEIKNAVKRYASEVKTGAFPDAEHSFK"
     gene            complement(1150..2049)
                     /locus_tag="PLQ77_02795"
     CDS             complement(1150..2049)
                     /locus_tag="PLQ77_02795"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPL09641.1"
                     /translation="MKKKSSLTFAVVGTGMVGTAIGFLLQKSGHNVAAVADLSAAHLK
                     RAASYIRAPGFHHPAQAAKLADCILITTPDGRIAAACAQIVSGSDVKGKKVFHMSGAG
                     GLDLLAPAARAGALTASIHPLQSFSSIDGAVSSIPGSYFGVTAAAGAKRLAADIVRDL
                     QGIPIPVSAAQKPLYHVAACIASNYLVSLLSVVEAVYRSIGFSEKDARRAYLPLVYGS
                     LKNIENQGCPNALTGPIARGDSETIQKHIDAMARHLPDFASMYAHMGAIAVKLAREKG
                     TLSAAQAKEILGMLKGAKHEHAD"
     gene            2260..2904
                     /locus_tag="PLQ77_02800"
     CDS             2260..2904
                     /locus_tag="PLQ77_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPL09642.1"
                     /translation="MKKVELMIFDFDGTLADTGADIAASVNHALRALGLASRPVGEIL
                     SFVGDGVRILMERALGDDHRSRLEEAMEFFLQHYENHLLDHTKLYPAAEEVLNHFRSK
                     KKVILTNKRYYLTVAIAKGLKIDRHFLEIVGADSTPFQKPDGRVISCLLEKYDVAPHR
                     AVMIGDGRNDVEAARNAGTLSCVHLNGLGNREAMLKMGADAYVERLTEIKSLFQ"
     gene            2907..4316
                     /gene="tilS"
                     /locus_tag="PLQ77_02805"
     CDS             2907..4316
                     /gene="tilS"
                     /locus_tag="PLQ77_02805"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPL09643.1"
                     /translation="MIRKVEQTIRKYSMLEKGDRVVVALSGGADSCALLGALAALAPP
                     WRLELIAAHFNHGLRGEESDGDEAFCRSLAGKTGLVFATQKLAETAIPAGLSPEDYLR
                     RERFRFLDRTAEDYRANKIALGHHRDDQAETFLINMIRGSGLDGLKGFLPVRENRYIR
                     PLIGVSREEILGFLRERGAGFREDASNSEGIYLRNRVRLDLIPFLREKFNPQMEQTLA
                     RTAEIIRRDDSFIDECVRKVLCLPHIRKEENEISFSASSVLSLHDALVYRVLKSLLEG
                     LAAGEGGFSFSHLQALAGLLKESRTGKRLSLPHGIAARREYDRVILTAEPEESSPRYE
                     YPLMIPGEVEVRERRMIFSIRREKEVPVNLKDRSAFFFDGDKIREPLVVRNRREGDRF
                     EPLGTKGSQKIKKLFMDRKIPRRERGRIALLADRESVIWIENMHFSERVKVTPETKNL
                     LVLEVRPKTAGRRPAQENI"
     gene            4375..6189
                     /gene="ftsH"
                     /locus_tag="PLQ77_02810"
     CDS             4375..6189
                     /gene="ftsH"
                     /locus_tag="PLQ77_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPL09644.1"
                     /translation="MNQAQKNIALVLTVLLVFLLVWQLFNQQNTTTKEITYTDMLTHL
                     ESGEITEVTIQGEAVSGKLANGSVFKTYAPKDDGLVGMFREKNVKITAKPLEENPWYM
                     ILVSWFPMLLLIGVWIFFMRQMQAGGGKAMSFGKSRARLITDKSKKVTFADVAGVNEA
                     KAELEEVIDFLKDPKKYTKLGGRIPKGLLLVGPPGTGKTLLARAIAGEADVPFLSISG
                     SDFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFESNEGVILVSATNRPDVLDPALLRPGRFDRQVVVPLPDVKGREKIFEVH
                     ARRLPVADDVNYAILARGTPGASGADIENLVNEGALNAARLNKEKVNMADFEFAKDKI
                     LMGTERKSMVISDLEKQHTAYHESGHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLP
                     VDEKHTYPSRYLENNIAVLLGGRAAEELILKDFTTGAGNDIERATNLARKMVCEWGMS
                     KTMGPLSYGKKEEQIFLGREFATHKDYSEETARMIDEEVVSIVKRNYDKAVSLLAEHI
                     EILHRMSRELLEKEVLNASEIDEIIGTALPVEHAAQTS"
     gene            6207..7412
                     /gene="folP"
                     /locus_tag="PLQ77_02815"
     CDS             6207..7412
                     /gene="folP"
                     /locus_tag="PLQ77_02815"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPL09645.1"
                     /translation="MKIVAIHHDGEALALFQKIGVDPYGIGAMTPKTRHVNILLSARP
                     CKVANILKQEMLSLGADAAVSRGSVSCAVEATDVLLMGTHKQIGALAGKIENQPFGLD
                     VLARDLRVLLGRLEKKPLLLKTVKREIVLGERTLIMGVLNVTPDSFSDGRRYLNFSSA
                     VNRGLQMAEEGADIIDIGGESTRPGAKTVALKEEIARVVPVVEELSAKLTIPLSVDTM
                     KAGVADKALKAGAEIVNDVSALSGDRKMASVVRKASAALVLMHMRGIPADMQEGDLRY
                     ADLTGEIFMFLEKAVQKALAAGISRERLVVDPGIGFGKTHADNCRILNKLCEFRALGL
                     PLLVGTSRKAFIGSVTGDAVDRRMEGTAATVAAAVMNGCHIIRVHDVAAMKKVAAMTD
                     AIVRAGMDS"
     gene            7409..7819
                     /gene="acpS"
                     /locus_tag="PLQ77_02820"
     CDS             7409..7819
                     /gene="acpS"
                     /locus_tag="PLQ77_02820"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPL09646.1"
                     /translation="MIYGIGIDLVENDRIEKIIRKWGPKFLSRVFTDAEIEYCGRHAQ
                     SSLHYGARFAVKESFLKAIGTGLGGGVKLAEIEVANLKSGKPGLRLSGGARAFVEQAG
                     IQKIHLSITHTKHYASAAVLLEKELPDGGFADKT"
     gene            7816..8295
                     /gene="tsaE"
                     /locus_tag="PLQ77_02825"
     CDS             7816..8295
                     /gene="tsaE"
                     /locus_tag="PLQ77_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPL09647.1"
                     /translation="MNRAEIELVSENPGQTFEIGLSIGKRAADGDVFALSGELGSGKT
                     CFTGGLARGLGIGEDYAITSPTFTLINEYSGRCRLYHCDVYRLSAAEELADLGFDEYL
                     SGKGVAAIEWAEKIPEALPPDAVFVSFAYVDENRRKIRISGPAQRVRDLLRDISREV"
     gene            8300..9520
                     /locus_tag="PLQ77_02830"
     CDS             8300..9520
                     /locus_tag="PLQ77_02830"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPL09648.1"
                     /translation="MGLVVQKFGGTSVANVEKIKNVAGKAIREKQAGHDVIVVLSAMA
                     GETNRLIDLAQSAAENPDEREYDSLISTGEQVTISLLSITLNSMGYPARSFLGFQVKI
                     LTDQAHQKARISAIDTEAIQKELKKGVIVVVAGFQGVDEENNITTLGRGGSDTSAVAL
                     AAALKADRCDIYTDVDGVYTTDPNICAKARRLERISYDEMLEMAMTGAKVLQPRSVEM
                     AKKYNVPVYVKSTFSDKGGTLVTKEDKEMEKEVLSGITYDRDQAKITVIHIPDRPGIA
                     ANLFLPLSERHISVDMIIQNASAQGFTDLTFTVAKKDMKAAQKIVEATAKEIGAKKVE
                     VDDDVAKVSIIGVGMLSHSGVAAKMFKTLADEGINIMMISTSEIKISCVIQRKYTELA
                     VMVLHDAFHLDKKK"
     gene            9524..10090
                     /locus_tag="PLQ77_02835"
     CDS             9524..10090
                     /locus_tag="PLQ77_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPL09649.1"
                     /translation="MKWHEHQIRGVVALGVVLALTSLILFAAPSLIRPKHPLLSESGP
                     QTLAVELANPNGMSGIFFVKPGTSLRSLCGRLEIPAPTGRDVVLQSGMRVRFATDENG
                     ESVRIESMDAATRLALNLPLDVNRAEVEDLRMIPGVGERLASDIVAMREKTGRFNRLE
                     ELMQIKGIKEKKLEKLRSHLYIDGPQGL"
     gene            complement(10085..10771)
                     /locus_tag="PLQ77_02840"
     CDS             complement(10085..10771)
                     /locus_tag="PLQ77_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPL09650.1"
                     /translation="MKSIRDLTGHFPLELPAPFQYDKTMIRKTNIKICPLAGGLIVLF
                     FFLVFLPAAPSHADIYKYEDPDGVIHLTNVPSNPKVKYVMLVREKRIIFSPGINVREY
                     DKIITQAASKFKLDASLIKAVIKAESNFNHKAVSPAGAKGLMQLMPQTASALNVENVF
                     HPENNIEGGARYLRYLLNLYKGNLTLALAAYNAGEGAVAKYNYGVPPYRETQNYVRRV
                     LDLYKSYSKL"
     gene            11033..11109
                     /locus_tag="PLQ77_02845"
     tRNA            11033..11109
                     /locus_tag="PLQ77_02845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11067..11069,aa:Pro,seq:ggg)
     gene            11267..11351
                     /locus_tag="PLQ77_02850"
     tRNA            11267..11351
                     /locus_tag="PLQ77_02850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11301..11303,aa:Leu,seq:tag)
     gene            11399..12724
                     /gene="tig"
                     /locus_tag="PLQ77_02855"
     CDS             11399..12724
                     /gene="tig"
                     /locus_tag="PLQ77_02855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPL09651.1"
                     /translation="MSELSVKVEDLSQVKKKMSFEIPWAFVKDELDAVYRTIGKKAKI
                     KGFRPGKIPRKVLETYFKSDAEGETITNIVNRYYWQELDQRKMVSLSRPEIEQNGLKE
                     DTDFSFSASFETEPEIDPQGYQGIELEKTEIRVSDADMDKRLQEIRKMFASLQDVGED
                     RPAAMGDFVTIDFAGTLDGEALPELKAEDYLLELGSKRFVPGFEEQVAGMKKGETLDI
                     QVKFPDDYTEQKMAGKDVTFTVAVKELKAQKLPEIDENFIKNFDKYNTLEELKEDVRK
                     SMEEQCKRQSDVKFKELITDVALKANEFEAPPSLVERQIFYLIADTQKRMKAAGMDEK
                     SAMELSFRMHDQFKADAEKTVKSFLLFKKIAEKEAITVSDDDIDKHIGELAQIHHMNV
                     ESVRKIYEDEEKKESLKADILQKKIFDFIEERANIKVVEKIGMGEEAAE"
     gene            12721..13323
                     /gene="clpP"
                     /locus_tag="PLQ77_02860"
     CDS             12721..13323
                     /gene="clpP"
                     /locus_tag="PLQ77_02860"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPL09652.1"
                     /translation="MNLIPMVVEQDGRGERAFDIYSRLLKDRIIFLGTAIDDNVANVV
                     VAQMLYLEAEDPDKEIFFYLNSPGGHVSSGMAIYDTMQYIKPHISTVCMGQAASMAAL
                     LLAAGEKGKRFALPHSRILIHQPLGGFQGQATDIDIQAREILRLKEDLNKILAELTGQ
                     PLEKVMNDTERDYFMTSRQAKDYGLIDEIVVRKPLESGVK"
     gene            13372..14595
                     /gene="clpX"
                     /locus_tag="PLQ77_02865"
     CDS             13372..14595
                     /gene="clpX"
                     /locus_tag="PLQ77_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPL09653.1"
                     /translation="MLFCSFCGKMQSEVRKLVAGPTAYICDECIELCRCIVEEDEKTV
                     EKGHKNVLPEPKEIKRFLDSYVIGQEKTKKILSVAVYNHYKRLFSGAGSNDGIEIQKS
                     NVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDVENIILSLLQNADY
                     DVARASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIMEGTMANIPPKGGRK
                     HPQQEFIQVDTTNILFICGGAFNDLETIISRRIGTNTMGFGAEIKSKKERRVGDVLAE
                     VRSEDLLKFGLIPEFIGRLPVIATLDDLEEAALVRILVEPKDAIIKQYKKLFELENVN
                     LKFTDGALRCVAKLSVERKSGARGLRSILENTMLEVMYDIPSQRDIKECVISEEVVLN
                     QEKPILIYDQKSESA"
     gene            14621..17029
                     /gene="lon"
                     /locus_tag="PLQ77_02870"
     CDS             14621..17029
                     /gene="lon"
                     /locus_tag="PLQ77_02870"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPL09654.1"
                     /translation="MTEEKDNTRTGTSNIIPLLPLRDVVVFPHMIVPLFVGREKSIAA
                     LESAMKYEKGIFMVAQKNAKKDDPSEDDIYSVGTIGIIIQLLRLPDGTVKVLVEGKAR
                     GAILEYVKNDDFFLVRVADIEDVDDDPNDVRKQALMRSIKESFELYLKLSKKVHVEMM
                     GTIAGIEDPSKLADVVVTHLNVKLEDKQRIMEIFSIGERLEAIYSLMLSEIEILQVEE
                     KIKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFKNEIAELEKRLKQKKLSEEAAK
                     KVRQEIKKLQMMAPMSAEATVVRNYIDWLLDMPWSEVTENQYTLKESEAILDEDHYGL
                     KKVKERIIEYLAVQSLVKKNKGPILCLVGPPGVGKTSIAKSVARATNRKFIRISLGGV
                     RDEAEIRGHRRTYIGAMPGKIIQSLKKAGSNNPVFCLDEVDKLCSDFRGDPSSALLEV
                     LDPEQNVAFNDNYLDVDYDLSDILFITTANVLQTIPAPLQDRMEVIRIAGYTEQEKMQ
                     IAKRFLLAKEMEANGLTPESIEFTNQALLRIIRQYTREAGVRNLEREIASICRKVAKE
                     IVSNGNRRKIVISSKNIPKYLGVPKFRHGETEENSQVGLTTGLAWTEFGGELLVIEAS
                     IMEGTGRMVMTGKLGDVMQESVQAALSYVRTRAEQFGLPKNFYKKIDIHVHVPEGAIP
                     KDGPSAGIAMATSIASALLKKKVRADLAMTGEITLRGRILPIGGLKEKILAAHRGNIK
                     IVLIPKDNEKDLAEVPRNVQNAMKIVFVEHIDEVLEIALVKKDDLFGNAVGNDVQAGS
                     PSVN"
     gene            17108..17183
                     /locus_tag="PLQ77_02875"
     tRNA            17108..17183
                     /locus_tag="PLQ77_02875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17141..17143,aa:Val,seq:tac)
     gene            17219..17295
                     /locus_tag="PLQ77_02880"
     tRNA            17219..17295
                     /locus_tag="PLQ77_02880"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17253..17255,aa:Asp,seq:gtc)
     gene            17529..18344
                     /locus_tag="PLQ77_02885"
     CDS             17529..18344
                     /locus_tag="PLQ77_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPL09655.1"
                     /translation="MKKKVFNLLSWNSPEQVRRELFSVLAKARKSGVVDDASRKMIEN
                     ILNFTSTLVREIMIPRTDIVSISADDEPQDMMREMVEAHYTRIPIHRGSVDNIIGILN
                     VKDLLAAWSPQMSAAEILSHMTRPYYIPETKNAHLLFYELKNNNKHIAIVIDEYGGTA
                     GLVTLEDLLEEIVGDIRDEHEEHADSEELTQMADGSIVADGRTEIEKIEEQLAIQLDR
                     GRYETVGGLILYAIRRIPHQGENFQIEGMDITIENADERSIRKVKIKKAENTP"
     gene            18341..19912
                     /gene="lnt"
                     /locus_tag="PLQ77_02890"
     CDS             18341..19912
                     /gene="lnt"
                     /locus_tag="PLQ77_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPL09656.1"
                     /translation="MKNNGTNINRNRTGKMPASRDLFFALLCGLLYFLSFPKFGFGIV
                     AWIALVPLFIALNNASSLSRALVLGWAAGITAHIGLLYWISHVVVNYGYLPMSLGVIL
                     TILLAGYLSVYFALFAAGIVYFRGRVPLLLAAPVLWVCLEHVKSVLFTGFPWENLGYS
                     QHANLLFIQTADITGVPGLSFLIVLLNVVIFTLLTQKSKKSLAAALAVFLLWSGVYAY
                     GALRLRQIQESMNHAERMDVSLIQGNIDQAVKWNENFQAETIRIYEELSLRKAMAPEG
                     LVVWPETAVPFSFQEPGVLQERVSRLPSKTKSWLLFGSISYAAGSAGTDYFNSAFLLS
                     PKGELRGKYDKVHLVPYGEYVPLRKLFPFVGSLASGIGDFAAGKGFAPLRMDSQKIGV
                     MICYEGILPEAARRYKNAGAGLLLNITNDAWFGRTSAPYQHLSMSVFRAVETRLCLAR
                     AANTGISAIVDPTGRILARTGLFEPDQINGRIPMIQTPTVYAEYGDWFVWACFAGLAG
                     LFIWSLKRREKNVSG"
     gene            19899..21009
                     /gene="prfB"
                     /locus_tag="PLQ77_02895"
     CDS             join(19899..19970,19972..21009)
                     /gene="prfB"
                     /locus_tag="PLQ77_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPL09657.1"
                     /translation="MSADSLQETIADLKKRIASIGECLDVGELELQVKDLEILCAKKD
                     FWDDPEKARSNLRKKTKLSESLESWQKFSRRIRETGNLWSMAFEENDEGLLADLEADL
                     RKLSAEVREEELKMMLASEQDPMNAIISIHAGAGGTEAQDWVEMLLRMYLRWAEKRNF
                     KTAIIDYLPGDEAGVKSVSFTVEGEYAYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEIDDEIKIDIRDDDLRIDTFRSGGKGGQHVNKTDSAVRITHLPTGIVVQCQNE
                     RSQHKNKSMAMKYLKSRLYERELQQKNEKLDEENKQKKDIAWGSQIRSYVLHPYKLVK
                     DHRTNLEIGNAQKVLDGEIDDFIQAYLMAVSNRKS"
     gene            21197..22873
                     /locus_tag="PLQ77_02900"
     CDS             21197..22873
                     /locus_tag="PLQ77_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPL09658.1"
                     /translation="MRKYKKYNLSRGLLIFLFMIVGFGFAPADRAEAQLPEVSVPFQN
                     PQSVSSPESQAGPPANPAVREDPNAAEEDSADLEEEQDLMENVLAILEVADNYWKNGD
                     IENTLNELDKAYALLLDTNGDVEIARQKDDLRLLISQRIIAVYSSKKGPTNGKASEIQ
                     LIMNPDVEKEIRSFQGPEREFFTSSYLRSGLYRELIVSELKKAGVPEELFWLPLVESG
                     FKVSAYSPARALGLWQFIPSTGYKFGLTRDEWVDERMDVLKSTRAAIAYLKELHAMFG
                     DWLTVLAAYNCGEGRVLRVISSQRINYFDRFWDLYSRLPHETARYVPRFLATLHIVRN
                     PAKYGFELQTSTDTLMNYETVKVGKMMKLSDVAAKMEVSEDYINVLNAELRHKITPDR
                     EYAFKIPEGSQQKFQLAYNDIPAAEKPGNHYGSDTGKSSKAGAGRYIRHRVRPGETVA
                     SIAKRYRVSRQAIYSCNRINKRKSLSRQTRYVRIPIHMAESPAVRQKASGGKSYAASG
                     SMKTYKVKKGDTLFRIATRFNVPVAKLKQANRLKGNAIKYGMTLKIPDQG"
     gene            complement(22864..23334)
                     /gene="ybeY"
                     /locus_tag="PLQ77_02905"
     CDS             complement(22864..23334)
                     /gene="ybeY"
                     /locus_tag="PLQ77_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPL09659.1"
                     /translation="MKIRIANLQKRLKIDNRRIRGRVARLLRLIDCAEKEISITFVDD
                     EAIRLLNRQYLRLDKPTNVLSFSLKEGECGMVNPGLLGDIVISADTALRDARKGSLTL
                     EEELLFLIIHGLLHLTGYDHVRTSRANALKMKKREQELFLLLTHSDIRSGGCHP"
     gene            complement(23288..25618)
                     /locus_tag="PLQ77_02910"
     CDS             complement(23288..25618)
                     /locus_tag="PLQ77_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPL09660.1"
                     /translation="MNNFLDSTSEKILSFFRKWKEKHPFSFSFLKDVIFQRWAIAILL
                     SAGLTALLAPQFYVSKPVYRHGMIVQENIKADREFLVEDIQSTRLKQTEAAAGVRPVY
                     EYDPETAMTIRAKIRQSFAQAAGQPEKPDGKTLLENSLGVALTQEEYAFLKANAFSDE
                     LLQKLSRVIFAFYDKTLVTASAFQNHEREKGITIVNTLTRQEENTKEVSSILHMKDTG
                     PALLRKVHASIRSDQPDARRASFSFLKKMIAPNLIRSPEATAQKTASAKEAVKPVYFQ
                     VQKNEMIVREGDKIGYLELAKLEALHKTAADNNISNLAVIAGIFCIVLFLTLLLYFWR
                     TRNWLKTSPRSNVDFLVFAIVALLQIFVVKAGIFISVAVNRAFPSLPVDACYFAIPFA
                     MGAMIIAVLVNRNVAMIMSVLTSFLISLLFEEKILFPLFSFLGSVAASYHIVNSRQRS
                     TFLKVGIFLGVVNIAAILSLNLLTGRLLEDLVLRLAMGLFGGLITGILVAGFTPVFES
                     LFGFITYIKLLELANLNQPLFQKMIIEAPGTYHHSIIVASLVESAAEAIGANALLAKV
                     SAYYHDIGKLAKPHYYIENQTGYDNRHDRLSPKMSALIIISHVKEGCELALQAKLGKP
                     IIDIIREHHGTSLISYFFDKAKKDKDESIRSLTESDFRYPGPRPQTKEAGLVMLGDVI
                     EASSRTLSNPTPARIRSLVRERIARIYTDGQLDECELTLSNLNTIAETFTRILTGIFH
                     HRVEYPEAPVKPKETNGKRESHENPNRKPAEAVENR"
     gene            complement(25627..26619)
                     /locus_tag="PLQ77_02915"
     CDS             complement(25627..26619)
                     /locus_tag="PLQ77_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPL09661.1"
                     /translation="MTKSTKSARAEKISFADSNLLRNLCGEHDRHLKLLEKLEDVKIK
                     PWGNTLSISGEEAAVAVVSGVIRQLCDLMLKGLHVELSDFDYAHRILSQDIDFDLSHV
                     FLDTVFISSKKRVIAPKSVAQRRYIDSMRNADMVLAIGPAGTGKTYLAMAMAVSCLLE
                     RKVQRIILTRPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMVDMEKANALIGKGL
                     IEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFCSKAVVTGDITQIDLPT
                     ERSSGLVEAAAVLRNIPAVRIVHFSQVDVVRHPLVADVISAYASWEKENKNQKS"
     gene            complement(26748..27992)
                     /locus_tag="PLQ77_02920"
     CDS             complement(26748..27992)
                     /locus_tag="PLQ77_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPL09662.1"
                     /translation="MMIDEVLFEKVSRRIDAWRGDMIDLQKALTAVPAVGPANGGDGE
                     MPKARTLQKHLSDMGFTIFRHFDAPDPSVSDGLRPNFLASIPGTNNARSVWIITHLDI
                     VPPGEPGLWSADPYHAYVKDDCVYGRGTEDNQQDMAASIFAARALLAEGIVPRSTINL
                     LFVSDEETSSEKGVQHLLALPEKIFGSGDWIVAPDSGDSEGSLIEVAEKGILWLGFKT
                     TGKQCHGSKPQLGINAFSAASSFVTQLTKLREIFDAQDPLFDPPVSTFEPTKKEANVG
                     NINTIPGEDVFCMDCRILPRYKLDDVLFEIEAIKREVEKNHGVTIEISIHQRVDPAPS
                     TPDDAPVVLALQNAVDRVYRVRAAAGGVGAGTVAAYLRKRGLPAAVWSKTNMKAHQPD
                     ENCPVDNMTGNAKVFAHLFLQA"
     gene            complement(28004..29434)
                     /locus_tag="PLQ77_02925"
     CDS             complement(28004..29434)
                     /locus_tag="PLQ77_02925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPL09663.1"
                     /translation="MKSKKTIFCTAGLLLALIFLAAPQAAHTAPPWIPPDQIRYPALE
                     FRMPQAQRIALKNGMFLFYLQDSELPLVSLTALVRTGTLHDPAGKEGLAEITASVMRT
                     GGTAKLGSGEMDRRLDFLAASPSISMSLDSASIHFIFLKSDLDECLDLLSRMLLEPAF
                     EEKKIQEALALKKEELRRTVDNPQTLAFREFNRLLYPGDPRGRYATSASLDNISRKDL
                     ISFHREYFSPANMLLAVSGDISREEAVKKIEQVFGDAKIPRLVHDIPAAPRQGGRGFF
                     LIRKPIPQSTVVGGELTISKNHPDFYAFEVLDFLIGSGGFSSRIFSAVRNEEGLAYSA
                     GSFYRARSNYGVFGTYAFTKTASTFQAISLIESILRDAAAGSIARAELDWAKRSIINS
                     FIFSFEQPRQIAFEQMKNEYEKLPADYLTTYREKIEAVTLEDIRRIAAAFLHENRRLT
                     LIVGDADRFGAWPENWPAPAQIVPQP"
     gene            complement(29424..>30418)
                     /locus_tag="PLQ77_02930"
     CDS             complement(29424..>30418)
                     /locus_tag="PLQ77_02930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPL09664.1"
                     /translation="NKIELWARIESDRLLHPVFRQFYTERDVILEERRQRVEASPDGK
                     LYEAFLGRAYTTHPYGIPIIGLPMDLTFLSPAAIRGIHRKYRSPGRICIAVVGDVDPS
                     KTMDLIRRYFGRIPDAGAPSARIPSEPEQRAERKVEVEFDASPSMIIGYHKPAPPAQD
                     DYVLDVLEAILGKGRTSRLYDKLVTERQIADSVSVVNGVPASRYSNLFTIFARPRHPH
                     TNEELQAAIIEEIESIKTHSVTEEELARAKNQLKMDYIKSLDSNSELASILSYYELLL
                     GDYRYFSDYLPRIQKVTAGEVRAAAVRYLTAENRTVAFLNRKPASRKGAETHEK"
BASE COUNT         7130 a         7893 c         8961 g         6434 t
ORIGIN      
        1 ggccattccc taccgcgacg aatacatcat cgccgtcggt tcgagctttg aaaaatacct
       61 ggccagcctg gataagcttg ccggcctgga cgtccgaatg atttgcgctg accattacgg
      121 atgcgttgag ggagttgagg cgagggaata catcgggcaa accagagccg cggcgcttca
      181 gatgatggac cggatgcgcg aggcgctggc gcaggaggga agcgtcgagg ccgcggcccg
      241 gcaattgatc aggcttcact acgaacgaag gccggattac tttgtccatc cggacattct
      301 gcttagaacc tacacgcaaa tgctcaaaca actcaccgga taaaaaacta tttgaagctg
      361 tgctccgcgt ccggaaaagc gccggttttc acctctgagg cgtagcgctt gaccgcgttt
      421 ttgatttcct ggctcagatg ggcgtatctt ttgacgaatt tgggcgtcat tcggtcgttc
      481 atgcccagca tgtcgtttac gaccaggacc tgcccgtcgc aggaaacccc cgcgccgatg
      541 ccgatggtcg gaatggacag gatgcgggtg atttccgcgg ccagcttttc cggcacgcat
      601 tccagcacca cggaaaaggc gcccgcttct tcgagaatca cggcgtcctt catcattttt
      661 tccgccgcgt cctccttctt gccctgtact ttgtagccgc cgagctggtg gaccgattgc
      721 ggcgtaagcc ccagatgcgc catgaccgga atgccggaga gcgtcatttt acggacgatt
      781 tccgcgtgct cccggccccc ttcgagtttg acggcctgag cgttgccctc ttttaaaaaa
      841 cgtccggcat tggccagcgc cgcctcctgc gacacctggt aggaaagaaa aggcatgtcc
      901 gccacaatca gcgccttgac cgatccccgg gccaccgctc ttgtgtgatg gatcatgtcc
      961 tccatcgtca cgggaagcgt ggtgtcgtag cccagaacga cattgcccag agaatcgccg
     1021 acgagcagaa tgtcgatgtc gctttcgtcc agaatggacg ccatgccgaa gtcgtaggcc
     1081 gtcagcatgg tgattttctc gccctgcgtt ttcattttct tgatatccag aatcgttttc
     1141 cggctgatct tagtctgcgt gctcatgttt tgctcctttc agcattccca gaatttcctt
     1201 ggcctgagcg gcgctcagtg tgcctttttc cctggcgagt ttcaccgcga tggcgcccat
     1261 gtgagcatac atcgaagcga agtcgggaag atggcgtgcc atggcgtcga tgtgtttctg
     1321 aatggtttcc gaatcgccgc gcgcgatcgg tccggtgagg gcgttcgggc agccctgatt
     1381 ctcaatattt ttcaaactgc cgtacaccag cggcagatag gccctgcggg cgtctttctc
     1441 cgaaaaaccg atggacctgt aaacggcttc gacgacgctc aaaagcgaga cgaggtaatt
     1501 cgatgcgatg caggcggcca cgtggtacag aggcttctgc gcggcggaga cgggaatggg
     1561 aatgccctga agatcgcgga cgatgtccgc cgccagtctc tttgcgcccg cggcggccgt
     1621 gacgccgaag tagcttcccg gtatgctgga aacggctccg tcaatggagg aaaaactctg
     1681 cagcggatga atgctggcgg tgagagcgcc ggcccgggcg gcgggggcga gcagatccag
     1741 gcctccggcg ccgctcatgt gaaaaacttt tttccccttc acgtccgagc cggaaacgat
     1801 ctgggcgcag gcggccgcga tgcggccatc cggcgtcgtg atcagaatgc agtcggcgag
     1861 cttagccgcc tgggccggat gatgaaaccc cggcgcccga atgtaggacg cggcgcgttt
     1921 cagatgagcg gcggaaagat cggcaacggc cgcgacgtta tggcctgact tctgcagcag
     1981 aaatccgatg gctgtgccga ccatgccggt cccgaccacg gcgaaggtga gcgaagactt
     2041 ttttttcatt ttcacgtcca taaaaaaagg gtcttccgcg gcgtaaggaa gacccgtatt
     2101 tgacagtttt gtggcgactc ccgtctcagt ctgcaacaag tgatccaagc ggttggaagc
     2161 ttcctagcac acggggcctg agccgtcaaa taatttttcc ccgccaaaat agcttgtttt
     2221 tgcttgattt tgcggctaaa cagccaacag gggaacaaaa tgaaaaaagt ggagttgatg
     2281 atattcgatt ttgacggaac gctcgcggac acgggagcgg acattgccgc ttccgtcaat
     2341 cacgccctga gagcgctggg gctggcatca aggccggttg gggagattct cagttttgtc
     2401 ggagacggcg tgcggatttt gatggaaaga gcgctgggtg atgatcatcg ctcaaggctg
     2461 gaggaggcaa tggaattttt ccttcaacat tacgaaaatc atctgctgga tcacacgaag
     2521 ctctacccgg cggccgaaga ggtgttgaat cactttcgtt ccaaaaagaa agtcatcctg
     2581 accaacaagc gttattacct gacggtggcg atcgccaaag ggctcaagat cgaccggcat
     2641 tttctggaaa ttgtaggcgc ggattcgacg ccgtttcaaa agccggacgg gcgagtcatt
     2701 tcctgtcttc tggaaaagta tgatgtcgcc ccgcacaggg ccgtgatgat cggcgacggc
     2761 cgcaacgatg tggaggcggc ccgaaacgcc ggaaccttgt cctgcgttca tttgaacgga
     2821 ctgggcaacc gggaggcaat gctcaaaatg ggcgccgatg cttatgtgga gcgcctgacg
     2881 gaaatcaaat ccctgtttca atgacgatga tccggaaagt agaacagacc attcgaaaat
     2941 actccatgct ggagaagggc gaccgggtgg tggtcgctct gtccgggggg gcggattcct
     3001 gcgcgctgct tggggcgctt gcggctcttg cgccgccgtg gcgtctggaa ttgattgccg
     3061 cccatttcaa tcacggactg cgcggcgaag agtctgacgg ggacgaagcg ttttgccgtt
     3121 cgctggccgg aaagaccggt ctggtttttg caacgcaaaa gctggccgaa accgcgattc
     3181 ccgcgggcct gtccccggaa gactatctgc ggcgggaaag gtttcgcttc ctggaccgga
     3241 cggccgaaga ttacagggcg aataaaatcg cgctcggaca tcaccgcgac gaccaggcgg
     3301 agaccttttt gatcaacatg atccgcggaa gcggtctgga cggactgaaa ggctttctgc
     3361 ccgttcggga gaaccggtac atccgaccgc tcatcggcgt atcccgggag gaaattctag
     3421 gttttctcag ggagcggggc gcgggctttc gcgaggacgc atccaattcg gaagggattt
     3481 atctgcgcaa tcgggtccgc ctggatctga tcccctttct tcgggaaaaa ttcaatcctc
     3541 aaatggagca gacgctggcg aggacggcgg aaattatccg ccgcgacgac tcattcatcg
     3601 atgaatgcgt tcggaaggtt ctgtgcctgc cgcatattcg aaaagaggaa aacgaaattt
     3661 ccttttccgc ttcgtccgtt ctttcccttc atgacgcgct cgtttaccgg gttcttaagt
     3721 ctcttctgga gggcctggcg gccggggaag gcggtttctc cttctcccat cttcaggcgc
     3781 tggccggcct gctgaaagag agccggacgg gaaaaaggct ctctctgccg catggaatcg
     3841 ccgcccgcag agaatatgac cgcgtgatcc tcacggctga acccgaagaa agcagtccgc
     3901 gctacgaata tcccctgatg atccccggcg aagtcgaagt ccgggaaaga cggatgatct
     3961 tttccatccg gcgggaaaag gaagtccccg tgaatttaaa agaccgctcc gcgtttttct
     4021 ttgacggaga caaaatccgg gagccgctgg tggtccgaaa ccgccgggaa ggcgaccggt
     4081 ttgagccgct gggaacaaaa gggtcccaga aaatcaaaaa gctgttcatg gaccgcaaga
     4141 ttcccagacg ggagcgcggc cgcatcgcgc ttctggccga ccgggaatcc gtgatctgga
     4201 ttgaaaatat gcatttcagc gagagggtga aggtgacgcc cgaaacgaaa aacctgctgg
     4261 ttttggaagt ccggcccaaa accgcgggcc gccgccccgc acaagaaaat atttgagtta
     4321 aaccggatat tattataaag gttgcccaac agtttaattt caaggagaaa gatattgaat
     4381 caggctcaaa aaaacatagc gcttgtcctg acagtgcttc tggtattcct cctggtctgg
     4441 cagttgttca atcagcaaaa caccaccacg aaagaaatca cctacacgga tatgctgacg
     4501 catttggaaa gcggcgaaat cacggaagtt accattcagg gagaggccgt ttccggcaag
     4561 ctggccaacg gcagtgtttt taaaacctat gccccgaaag acgacggtct ggtcggaatg
     4621 tttcgtgaga aaaatgtcaa aatcaccgcc aaaccgctgg aagagaatcc ctggtacatg
     4681 attcttgtgt cctggtttcc gatgctgctc ttgatcggcg tgtggatttt cttcatgcgc
     4741 cagatgcagg cgggaggcgg caaggcgatg tctttcggaa aaagccgggc gcggctgatc
     4801 accgacaagt ccaagaaggt gacctttgcc gatgtggccg gcgtgaatga agccaaggcg
     4861 gagctggaag aggtaatcga ctttttaaaa gaccccaaga aatacaccaa actgggcggc
     4921 cgcatcccca aggggctttt gctggtgggg cctcccggaa cggggaaaac ccttctggcg
     4981 cgggcgattg ccggggaagc cgacgtgcct tttttgagca tcagcggctc cgatttcgtg
     5041 gaaatgtttg tcggcgtcgg cgcgtcgcgc gtgcgggatt tgttcagcca ggccaagaag
     5101 aacgctccct gcattatctt tatcgatgaa atcgacgcgg tcggacgtca ccgcggcgcg
     5161 ggactgggcg gcggccatga tgagcgggag cagacgctga atcagcttct ggtcgaaatg
     5221 gacggttttg agtccaatga gggcgtgatt ctggtttcgg cgaccaaccg tcccgacgtg
     5281 ctggatccgg cacttttgcg tccggggcgt tttgaccgcc aggtggtggt gccgctgccg
     5341 gatgtgaagg ggcgggagaa aatctttgaa gtgcacgcac gcaggcttcc ggtcgcggac
     5401 gacgtgaatt acgcgattct ggccaggggg acgcccggcg catccggggc cgacatcgaa
     5461 aatctggtca atgaaggcgc gctcaacgcc gcgagactga acaaggaaaa agtcaatatg
     5521 gcggactttg aattcgccaa ggacaagatc ctgatgggca cggaacgaaa aagcatggtc
     5581 atcagcgacc tggaaaagca gcacacggcc tatcacgaat ccggccatac gctggtggcc
     5641 cggctgatcc cgggaaccga cccgatccac aaggtcacga tcattccccg cggccgcgcc
     5701 ctgggattga cgcagcagct tccggtggac gaaaagcaca cctacccaag ccggtacctg
     5761 gaaaacaata tcgccgtcct gctgggcggg cgcgcagccg aagaactgat tctgaaggac
     5821 tttacgaccg gtgcgggcaa cgacatcgag cgggcgacca acctggcccg gaagatggtc
     5881 tgtgaatggg gcatgagcaa aaccatgggg ccgctcagct acggcaagaa ggaagagcag
     5941 atctttctgg gaagagaatt tgcgacgcac aaggattaca gcgaagaaac ggccagaatg
     6001 atcgacgagg aagttgtctc gatcgtcaag cgcaactacg acaaagccgt gtccctgctt
     6061 gccgagcaca tcgaaatttt gcacaggatg tccagggagc ttctggaaaa agaggtcctc
     6121 aacgcctcgg agatcgacga gattatcggc accgccctgc ctgtcgagca tgccgcgcaa
     6181 acttcataaa cggttgatgg cgaattttga agatcgttgc aatccatcat gacggcgaag
     6241 cgctcgccct gtttcagaaa atcggcgtgg acccttacgg gatcggcgcg atgacgccta
     6301 aaacgcggca cgtgaacatc ctgttgtccg cccggccctg caaggtcgcc aacattctca
     6361 agcaggaaat gctttctctg ggcgcggacg cggcggtttc gcgcggaagc gtttcctgcg
     6421 cggtggaagc caccgatgtt ctgctcatgg ggacccacaa gcagatcggc gccctggccg
     6481 gaaaaatcga aaatcagccc ttcggactgg acgttcttgc ccgggacctt cgcgtgcttt
     6541 tgggccgtct ggaaaagaag cccttgctcc tgaaaacggt gaagcgcgaa attgtgctgg
     6601 gagaaagaac gctgatcatg ggcgttttaa acgtgacgcc cgattccttt tcggacggca
     6661 gacgttattt gaatttctcc agcgccgtca accggggcct gcaaatggcg gaggaagggg
     6721 cggacatcat cgacatcggc ggtgaatcca caaggcccgg agcaaaaacg gtcgccctga
     6781 aggaagaaat cgcgagagtg gttcccgtgg tggaggagtt gtccgcgaag ctgacgatcc
     6841 ctctgtccgt ggataccatg aaagccgggg tcgctgataa agccctgaag gcgggagcgg
     6901 aaatcgtaaa cgacgtgagc gcgctttccg gcgatcggaa aatggcttcg gtcgtccgga
     6961 aagcctccgc ggcgctcgtt ttgatgcaca tgcggggaat cccggccgac atgcaggaag
     7021 gcgatctgcg ctatgccgat ctgacgggcg agattttcat gtttctggaa aaggccgttc
     7081 aaaaagccct ggcggcgggc atcagccggg agcggctggt ggtcgatccg ggaatcggct
     7141 tcggcaaaac ccacgcggac aactgccgaa tcctcaacaa gctttgcgaa ttcagggcgc
     7201 tggggcttcc gctgctggtc gggacctcgc gcaaagcgtt tatcggcagc gtgacggggg
     7261 atgccgtgga ccggagaatg gaaggcacgg cggcgacggt cgccgcggcc gtgatgaacg
     7321 gctgtcatat aattcgcgtc cacgatgtcg cggccatgaa aaaagtggcg gccatgacgg
     7381 atgcaatcgt ccgcgccggg atggactcat gatttacgga atcggcatcg atctggtgga
     7441 aaacgaccgc atcgaaaaaa tcattcggaa atggggcccg aaatttttgt cccgggtgtt
     7501 taccgacgcg gaaatagaat actgcgggcg gcacgcgcag tcttcccttc attacggagc
     7561 gcggtttgcc gtcaaggaat cgtttctcaa ggccatcggc acgggcctcg gcggcggcgt
     7621 aaagctggcg gaaatcgagg tggcaaacct gaaaagcgga aaacccgggc ttcgcctgtc
     7681 cggcggcgct cgggcgtttg tggagcaggc cgggattcaa aaaatccacc tcagcatcac
     7741 ccacacgaag cattacgcgt ccgcagcggt cttgctggaa aaggaattgc cggatggcgg
     7801 atttgcggat aaaacatgaa cagggcagaa attgagctgg tgtcggaaaa tcccgggcaa
     7861 acctttgaga ttggcctgtc catcgggaaa cgggcggcgg acggcgatgt tttcgctctg
     7921 tccggagaac tgggctccgg caagacctgt tttacgggcg ggctggcgag ggggcttggg
     7981 atcggcgagg actacgcgat cacgagtccg acctttacgc tgatcaacga gtattccggg
     8041 aggtgcaggc tttatcactg cgacgtttac cggctttccg cggcggaaga gcttgcggat
     8101 ctggggtttg acgagtacct gtcgggaaaa ggcgttgcgg ccattgaatg ggcggagaag
     8161 attccggagg cactgccgcc ggatgccgtt tttgtgtctt ttgcctatgt ggatgaaaac
     8221 aggcggaaaa taagaatcag cgggcccgcg cagagggtcc gggatttatt gcgggatatt
     8281 tcgagggagg tttaaaatta tgggtctggt tgttcagaaa ttcggcggaa catccgtggc
     8341 gaatgttgaa aaaatcaaaa atgtggcggg caaggcgatt cgcgaaaagc aggcggggca
     8401 cgatgtgatt gtcgttctct cggcaatggc gggagaaacg aaccggctca tcgacctggc
     8461 ccagagcgcg gcggaaaatc cggacgaaag agagtatgat tcgttgattt ccaccgggga
     8521 gcaggtgacg atttcgctgc tttccatcac gctcaattcc atggggtatc ccgcgcgttc
     8581 ttttctcggt tttcaggtca aaatcctgac ggaccaggcg catcagaagg cgagaatctc
     8641 cgcgattgac acggaggcga ttcaaaaaga gctgaaaaaa ggcgtcatcg tggtcgtggc
     8701 gggattccag ggcgtggatg aagaaaacaa catcaccacg ctgggccgcg ggggatccga
     8761 caccagcgcg gtggcgctgg cggcggctct taaggcggac cggtgcgaca tctatacgga
     8821 cgtggacggc gtttacacga ccgatcccaa catctgcgcc aaagccagaa ggctggagag
     8881 gatttcctac gacgaaatgc tggaaatggc gatgaccgga gcaaaagttc tccagccccg
     8941 gtcggtggaa atggccaaga aatacaatgt gcccgtttac gtcaaatcaa ctttttccga
     9001 taaaggcgga acactggtaa cgaaggagga taaggagatg gaaaaagaag tgctgtcggg
     9061 aatcacctac gatcgcgatc aggcgaaaat tacggttatt catattccgg accggccggg
     9121 catcgcggcg aatttgtttt tgcctttgtc ggagcgccac atcagcgtcg atatgatcat
     9181 tcagaacgcc agcgcccagg gctttacgga tttgaccttt accgtggcga aaaaggacat
     9241 gaaggccgcg cagaaaatcg tggaggcgac ggccaaagaa atcggcgcca aaaaggtgga
     9301 ggttgacgac gacgttgcca aggtttccat catcggcgtg ggcatgctca gccattccgg
     9361 cgtcgcggcc aaaatgttca aaaccctggc ggatgaaggc atcaacatca tgatgatcag
     9421 cacgtcggaa atcaaaatct cctgcgtgat ccagagaaaa tatacggagc tggccgtgat
     9481 ggtgctccac gacgcttttc acctggacaa gaagaaataa aaagtgaaat ggcatgagca
     9541 tcagattcgg ggagtcgtcg cgctgggagt tgtgctggcg ctgacctccc tgattctttt
     9601 tgcagccccc tccctgatcc gcccgaaaca ccccttgctt tccgaatccg gcccgcaaac
     9661 cctcgcggtt gaactggcga atcccaacgg catgtcgggc atttttttcg tcaagccggg
     9721 aacctcgctt cgatccctgt gcggccgcct ggagattccc gcgccgacgg gaagggacgt
     9781 tgttctgcaa agcggcatgc gcgttcgctt cgcgactgac gaaaacgggg agtccgtcag
     9841 gattgaaagc atggatgccg cgacaagact ggccctgaat ctgcctctgg atgtcaatcg
     9901 ggcggaagtg gaagacctga ggatgattcc cggagtgggg gagcggctgg cttccgatat
     9961 tgtggcgatg cgggagaaaa ccggccggtt caaccggctg gaggagctca tgcagatcaa
    10021 gggaatcaaa gagaaaaaac tggaaaaatt aagaagccat ctctatatcg acgggccgca
    10081 gggcttataa tttactgtag gatttgtaca ggtcaagaac ccgccgaacg taattctgtg
    10141 tttcccggta cggcggcacg ccgtagttgt acttggccac cgctccttcc cccgcattgt
    10201 atgcggccag cgcgagcgtc aggtttccct tgtaaagatt cagcagatag cgaagatagc
    10261 gcgccccccc ttcgatattg ttttcgggat gaaaaacatt ttccacgttc agcgcggagg
    10321 cggtttgcgg catgagctgc atgaggcctt tggcgccggc gggggagacg gccttgtgat
    10381 tgaaattgga ttccgctttg atgacggcct tgatcaggga ggcatccagc ttgaatttgc
    10441 tggcggcctg cgtgatgatc ttgtcgtatt cccttacatt gatgccgggg gagaagatga
    10501 ttcttttttc ccggaccagc atcacgtatt tgactttggg attggacgga acgttggtca
    10561 ggtggatgac gccgtcgggg tcctcgtatt tatagatatc cgcgtgcgag ggggcggccg
    10621 gcagaaaaac caggaagaaa aacaggacga tcagtcctcc cgccaagggg caaattttta
    10681 tatttgtctt gcgaatcatg gtcttatcat actggaaggg cgctggaagt tcaagcggaa
    10741 aatggcccgt caggtcacga atggatttca tcggcctgtt gcccaaatat ccttttcgct
    10801 tgcctgagag tgttttgtat aaatctaagg ctcacttcag gcaatgacaa aactcgcctt
    10861 cggctcagac agttgtcatt gctgatctta cgcttcgcca agatttatac aacaaaaccc
    10921 tctcaatggc cgctcaaagg gatattgggc aacaggcggt tgaatttgtg tttgttcttg
    10981 acaggtttgg ccgaaggtgg taattcatcc gccacttaac agcaaatcat gtcggggcgt
    11041 ggcgcagtct ggtagcgtat ctgaatgggg ttcagagggc cggtggttca aatccactcg
    11101 ccccgaccat ttttcccagt tcatctttct cttgcaatac agggaatttt actctacagg
    11161 atacggcctg caggaattgc aacatgactg gttttgcagc agaatgccga aaataatccg
    11221 cgttgacatt ttgaccaatg aatatataat cccgaagcca tcacgggcga gagtggcgga
    11281 attggcagac gcactggact taggatccag cgggtaaaac cttaggagtt caagtctcct
    11341 ctctcgcacc agaatgaaaa tgaaggagaa ttccatacaa aagaaggaga atcaaggcgt
    11401 gagcgagctt tccgttaaag tagaggacct aagccaggta aagaaaaaaa tgtctttcga
    11461 gatcccctgg gcctttgtaa aagatgaatt ggacgccgtt taccggacca tcggcaagaa
    11521 ggcgaaaatc aagggtttcc gtccgggcaa gattccccgg aaggtgctgg aaacctattt
    11581 caagagcgat gcggaagggg aaaccatcac caacatcgtc aaccggtact actggcagga
    11641 gctggatcag cggaaaatgg tgtcgctttc ccgtccggaa atcgagcaga acggattgaa
    11701 ggaagatacg gacttttctt tcagcgcctc ctttgaaacg gagccggaaa ttgatccgca
    11761 ggggtatcag gggatcgagc ttgaaaaaac cgagatcagg gtttcggatg ccgatatgga
    11821 taagcgcctt caggaaatcc gcaaaatgtt cgccagcctt caggatgtcg gggaagaccg
    11881 gccggcggcg atgggtgatt ttgtgacgat tgattttgcc ggcacgctgg acggcgaagc
    11941 gctgcctgag ttaaaagcgg aggactatct tctggagctg ggctccaaaa gatttgtccc
    12001 gggttttgaa gagcaggttg cgggaatgaa aaaaggcgaa accctggata ttcaggtcaa
    12061 atttccggac gattataccg aacagaagat ggcgggaaaa gacgtcacgt tcacggttgc
    12121 cgtaaaggag ctcaaagcgc agaaactgcc agagatcgat gaaaacttca tcaagaattt
    12181 cgacaagtac aacacgcttg aggaattgaa ggaagatgtc cgcaagtcga tggaagagca
    12241 gtgcaaacgg caaagcgatg tgaaattcaa ggagttgatc accgatgttg cgctcaaagc
    12301 caatgaattt gaagcgccgc cttccctggt ggagcggcag attttctacc tgatcgccga
    12361 cacgcagaaa aggatgaagg cggccggcat ggacgagaag agcgcgatgg agctgagctt
    12421 ccgaatgcac gatcagttta aggccgacgc ggaaaaaacg gtcaaatcct ttctcctttt
    12481 caaaaaaata gccgaaaaag aggccatcac ggtatccgat gacgatatcg ataaacatat
    12541 cggtgagttg gcccaaatcc atcacatgaa tgtcgaatcc gttcggaaga tctacgaaga
    12601 cgaggagaag aaagaatctc tgaaagcgga tatcctccag aaaaagatat ttgactttat
    12661 cgaggagcgg gccaatatta aggtcgtgga aaagatcgga atgggcgagg aggctgctga
    12721 atgaatttga tccccatggt ggtggagcag gatggacggg gcgaaagggc gtttgatatc
    12781 tattcccgtc ttttgaagga caggatcatc tttctgggga cggcgatcga cgacaacgtg
    12841 gccaacgtag tggtcgcgca gatgctttat cttgaagcgg aagatccgga taaagaaata
    12901 tttttctatt tgaattcgcc cggcggccat gtcagctccg gaatggcgat ttacgacacg
    12961 atgcagtaca tcaaacccca tatttcgacc gtgtgcatgg ggcaggcggc cagcatggcg
    13021 gcgcttctgc tggcggccgg cgaaaaggga aagcggtttg ccctgccgca ttcaaggatt
    13081 ctcatccatc agccgctggg aggttttcag gggcaggcga cggatatcga cattcaggcc
    13141 agggaaatcc tgcggctgaa ggaagatctc aacaaaatcc tggcggaatt gaccggccag
    13201 ccgctggaaa aggtgatgaa cgacacggaa cgggattact tcatgaccag ccggcaggca
    13261 aaagattacg gattaataga tgaaatcgtg gtccggaaac cgctggaatc cggcgttaaa
    13321 taaaggagat aggcagtgat caaaagaaac aaggacaatc gggggcaggg tatgcttttt
    13381 tgctcatttt gcgggaagat gcaaagcgaa gtacgcaaac tggtagccgg tccgacggcc
    13441 tacatctgcg acgaatgcat tgaattgtgc cgctgcattg tggaagaaga cgaaaaaacg
    13501 gtggaaaaag gccataaaaa tgttctgccg gagcccaagg aaatcaagcg gtttctggat
    13561 tcctacgtga tcggccagga aaagaccaaa aaaattctct ccgtcgccgt atataatcat
    13621 tacaaaaggc tgttttccgg cgccggttcg aatgacggga ttgaaattca gaagagcaat
    13681 gttttgctga tcgggcccac cggatctggc aaaaccctgc ttgcgaaaac gctggcaaaa
    13741 tttctcaatg ttccgtttac gatagcggat gccactacgc tcacggaagc gggctatgtg
    13801 ggagaggatg ttgaaaatat catcctgagc cttctgcaga atgccgatta cgacgtggcc
    13861 cgtgcctcaa aaggcattgt ttatattgat gaaatagata aaatcgcgaa aaaatccggc
    13921 aacccctcca tcacgcggga cgtctccggc gaaggcgttc agcaggctct tttgaaaatc
    13981 atggaaggaa ccatggccaa tatcccccca aagggcggcc gaaaacaccc gcagcaggaa
    14041 ttcattcagg tcgatacgac caatatcctg tttatctgcg gaggcgcctt caatgatctg
    14101 gaaacgatca tttcccggcg aatcggcacc aacaccatgg ggttcggcgc ggaaatcaaa
    14161 agcaaaaagg aaagaagggt aggggacgtt ctggcggaag tccggtccga agacctgctc
    14221 aaattcggcc tgattcccga attcatcgga cgtctgcccg tgatcgccac gctcgacgac
    14281 ctggaagaag cggcgctggt ccgcattctg gtggagccca aggacgccat catcaagcag
    14341 tacaagaagc tctttgagct ggaaaacgtg aacctcaagt ttacggacgg cgccttgcgc
    14401 tgcgtggcca aactgtcggt ggagaggaaa tccggcgcgc gcggcctgcg ctccattctg
    14461 gaaaacacca tgctggaagt gatgtacgac attccgtcgc agcgggacat aaaggaatgc
    14521 gtcatcagcg aagaagtggt gctcaaccag gaaaaaccga ttttgatcta tgatcagaaa
    14581 tccgaatccg cataaggcat aaccgccgca ggaagagaaa atgaccgaag aaaaagacaa
    14641 cacccgtacc ggcacatcca acatcattcc gcttttgccg cttcgcgatg tggttgtgtt
    14701 tccccatatg attgttccgc tgtttgtcgg gcgggagaag tccatcgccg ccctggaatc
    14761 cgccatgaag tatgaaaaag ggattttcat ggtggcccag aaaaacgcca agaaagacga
    14821 tccgtcggaa gacgatattt attcggtagg aaccatcggc atcatcatcc agcttttgcg
    14881 gctgccggac ggcacggtga aggtgctggt ggagggaaag gcgcgcggcg ccattctcga
    14941 atacgtgaaa aacgacgatt tcttcctcgt ccgtgtcgcg gatatcgaag atgtcgatga
    15001 tgacccgaac gatgtgcgga aacaggcgct gatgcgcagc atcaaggaat ccttcgagct
    15061 gtatctgaag ctcagcaaga aagtccacgt ggaaatgatg gggacgattg ccggcatcga
    15121 agacccttcc aaactggccg atgtcgtggt gacccatctg aacgtcaagc ttgaagacaa
    15181 gcagcggatc atggaaatct tcagcatcgg ggagaggctg gaggcgattt attcgctgat
    15241 gctctccgag atcgaaattc tgcaggtgga ggaaaagatc aagcggcgcg tcaagaagca
    15301 gatggaaaag acgcagaagg attactactt aaacgagcag atgcgcgcca tccagaagga
    15361 aatgggcgag aaagacgact ttaaaaatga aatcgcggag ctggaaaagc gcctgaagca
    15421 gaaaaagctc tccgaggaag ccgccaaaaa ggtccgccag gaaatcaaga aactgcagat
    15481 gatggcgccc atgtcggcgg aggccaccgt cgttcgcaat tacattgact ggcttctgga
    15541 catgccctgg tcggaggtca cggagaacca gtataccctc aaggagtcgg aggcgattct
    15601 cgacgaggat cactacggcc tgaaaaaggt caaggaacgg atcatcgaat acctggcggt
    15661 tcagtcgctg gtcaagaaaa acaaggggcc gattctctgc ctcgtcgggc ctcccggcgt
    15721 gggaaaaacg tcgattgcca aatccgtcgc gcgcgcgacg aaccggaaat ttatccgcat
    15781 ctccctgggc ggcgtccgtg acgaagccga aatccgcggc catcgccgga cctacatcgg
    15841 ggccatgccc ggcaagatca ttcaatcgct gaaaaaggcc ggatccaaca atcccgtttt
    15901 ctgcctggat gaagtggaca agctctgctc cgattttcgc ggcgatccct cttcggcgct
    15961 tctggaagtt ctggatcccg agcagaacgt ggcgtttaac gacaattatc tggacgtgga
    16021 ttatgatttg tccgatattt tgttcattac gacggccaat gttctgcaga cgatcccggc
    16081 gcccctgcag gaccggatgg aagtcatccg cattgccggc tacacggagc aggaaaaaat
    16141 gcagatcgcg aagcggttcc tgctcgccaa ggaaatggaa gccaacggcc tgacgccgga
    16201 gtccatcgaa ttcaccaatc aggccctgct caggattatc cgccagtaca cgcgggaagc
    16261 cggcgtgcgc aacctggagc gggaaattgc gtccatctgc cgcaaggtcg ccaaggaaat
    16321 cgtcagcaac gggaaccgca ggaaaattgt catcagctcc aaaaatatcc cgaaatacct
    16381 gggcgttcca aaattccgcc acggcgaaac ggaggaaaac agccaggtcg gtttgacgac
    16441 gggcctggcc tggacggagt tcggcggcga actgctcgtc atcgaggcct ccattatgga
    16501 agggacgggc cgcatggtca tgaccggcaa gctgggcgac gtgatgcagg aatcggtgca
    16561 ggcggcgctc tcgtatgtcc ggacgcgcgc ggagcaattc ggcctgccga aaaactttta
    16621 taaaaaaatt gatatccacg tccacgtgcc cgaaggagcc attcccaagg acggaccgtc
    16681 cgccggcatc gccatggcca cttccatcgc ctcggcgctg ctcaaaaaga aagtccgggc
    16741 ggacctggcc atgaccggag aaatcaccct tcggggacgg attctgccga tcggcggcct
    16801 gaaggaaaaa attctggcgg ctcaccgcgg aaacatcaaa atcgtcctga tccccaagga
    16861 caacgaaaag gatctggcgg aagtgccgcg caacgttcaa aacgcgatga aaatcgtgtt
    16921 tgtcgaacac atcgatgagg tgctggaaat cgccctggtg aagaaggacg acctcttcgg
    16981 caacgcggtg ggaaatgacg tgcaggcggg ttccccgtcc gtgaattaac ggggtttttt
    17041 tgttgtttta tcgcttgaca agccaactca atgttgctag aagccagaca gcttttttcg
    17101 ggcaaggggg cgggtagctc agctggaaga gcgccacgct tacaccgtgg atgtcacagg
    17161 ttcgagccct gtcccgccta ccaggaaaga aagtgaatat tgtttttaat gattttttgg
    17221 ggtcgtagtt aagttggtta taacgccggc ctgtcacgcc ggagggcgtg ggttcaagtc
    17281 ccatcggccc cgccagaaaa tctaaaaggg aatcagcttt tttgagtggt tcccttttta
    17341 tttcgcccca aagccgggcc gcggctttcc atcctgcgga aaacagcgat gccgcgcttt
    17401 gttcacccat cgccgatgaa cggcatctct gagcggcgat tttccgcggg gtgattgaca
    17461 gaacggatgt tgcgtggtta attgccgatg aaaattcacg cgggtcgcgc gcggggaaaa
    17521 tcgagactat gaagaaaaag gttttcaatc ttttgtcctg gaacagcccc gaacaggttc
    17581 gccgggaact gttttcggtc cttgccaaag cccgaaagag cggcgtagtg gacgacgcat
    17641 cccggaaaat gatcgaaaac atactgaatt ttacgtccac cctggtgcga gagatcatga
    17701 ttccccggac ggatatcgtg tcgatcagcg cggatgacga gccgcaggac atgatgcggg
    17761 aaatggtcga ggcgcattac acgcgcattc ccatccaccg cggctccgtg gacaatatca
    17821 tcggcatcct gaacgtcaaa gacctgctcg cagcatggtc gccgcagatg agcgccgccg
    17881 agatcctgtc ccatatgacg cggccctact atattccgga gacgaaaaac gcgcacctgc
    17941 tgttttacga actgaaaaac aacaacaagc acatcgccat cgtcatcgat gaatacggag
    18001 ggacagccgg gctggtcacg ctggaggacc tgctggagga aattgtcggc gatatccggg
    18061 atgaacatga agagcatgcc gattccgaag aactcaccca aatggccgac ggttccatcg
    18121 tcgcggacgg acggacggaa atcgagaaaa ttgaggagca actggcgatt caactggacc
    18181 gcggccggta cgaaacggtc ggcggtctga ttttatacgc gattcgcaga atccctcatc
    18241 agggcgaaaa ctttcaaatc gagggaatgg acatcaccat cgagaatgcc gatgaacgaa
    18301 gcatccggaa ggtgaaaatc aaaaaagccg aaaacactcc atgaaaaata acggcacaaa
    18361 catcaaccgg aatcggaccg gcaaaatgcc ggccagccgc gatctttttt tcgcgctcct
    18421 ttgcgggctt ctgtactttt tgtcctttcc caagttcggc ttcggcatcg tcgcctggat
    18481 tgcgctggtt cctcttttta tcgccctgaa caacgcttca tcgctttcga gggctctcgt
    18541 tctgggatgg gcggcgggca tcacggccca catcggcctg ctgtactgga tttctcatgt
    18601 cgtggtgaac tacggttatc ttcccatgtc tctgggcgtg atcctcacga tcctcctggc
    18661 gggctaccta agcgtctatt tcgctctgtt tgcagcgggg atcgtttact tccggggcag
    18721 ggttcccctg cttctggccg cgccggtttt gtgggtttgc ctggagcatg taaaatccgt
    18781 tttgttcacg ggctttccct gggagaacct gggatattcc cagcatgcca atctgctgtt
    18841 cattcagacg gccgacatca cgggggtgcc cggactgtct tttctgatcg ttcttttaaa
    18901 cgttgtcatt tttacactgc tgacgcaaaa gtccaaaaaa tccctggcgg cggcgctggc
    18961 cgtgtttctt ttgtggtcgg gagtttacgc ctacggcgcg ctgcgccttc ggcagatcca
    19021 ggagagcatg aatcatgcag agcgcatgga cgtttccctg attcagggca acatcgatca
    19081 agccgttaaa tggaatgaaa attttcaagc cgaaacgatt cgcatttacg aagagctgtc
    19141 cctgcgcaaa gcgatggctc ccgaaggcct ggtggtctgg cccgaaaccg ccgtcccttt
    19201 cagctttcag gagccgggtg ttttgcagga acgggtcagc cggctgccct ccaaaacgaa
    19261 aagctggctg ctgttcggat ccataagtta tgccgccggg agcgcgggca cggattactt
    19321 taacagcgcc tttctgcttt caccaaaggg agaacttcgg gggaagtacg ataaagtgca
    19381 tttggtgcct tacggggaat atgttccact gagaaagctg tttccctttg tcggcagcct
    19441 cgcgtccggc atcggcgatt ttgcggcggg gaaggggttt gctcccctgc ggatggacag
    19501 ccaaaaaatc ggcgtgatga tctgctacga gggaatcctg cccgaggcgg ccagacgata
    19561 taaaaacgcg ggggccggcc ttctgctcaa tatcacgaat gatgcctggt ttggacggac
    19621 gtccgctccc tatcagcatc tttccatgtc cgtttttcga gccgtggaaa cccggctttg
    19681 tctggcgcgg gcggccaata cgggcatctc cgcgatcgtg gacccgaccg gaaggattct
    19741 ggcgaggacg ggcctgtttg agcccgatca aatcaacggc agaataccga tgatccagac
    19801 gccgactgtt tacgcggagt acggcgattg gttcgtgtgg gcgtgctttg ccggtctggc
    19861 cggtttattc atttggagcc taaaaaggag ggagaaaaat gtcagcggat agccttcagg
    19921 aaaccattgc ggatttgaaa aaaaggatcg cttcgatcgg agagtgtctt tgacgtaggc
    19981 gaactggaat tgcaggtcaa ggatctggag attttgtgcg ccaaaaaaga tttctgggac
    20041 gaccccgaaa aagcccgttc caacctgcga aaaaaaacaa agctctccga atcgctggag
    20101 agctggcaaa aattcagccg gcggattcgg gagacgggaa acctctggtc gatggccttc
    20161 gaggaaaatg acgagggtct tcttgcggac ctggaagccg acctgcggaa actttccgcc
    20221 gaggtcaggg aagaagagct taaaatgatg ctcgccagcg agcaggatcc gatgaacgcg
    20281 atcatctcca tccacgcggg ggcggggggc acggaagcgc aggactgggt ggaaatgctg
    20341 ctgcgcatgt atctgcggtg ggctgaaaaa agaaatttta aaacggccat tattgattat
    20401 ctgccgggcg atgaagcagg ggtgaaaagc gtttcgttta ccgtggaggg cgaatacgct
    20461 tacgggtacg ccaaggcgga gatcggaatt caccgtctgg tgcgcatttc tccgttcgac
    20521 gctggcgcca gacgccatac gtccttcgcg tcggtatttg tctatccgga aatcgacgac
    20581 gaaatcaaaa tcgacattcg cgacgatgat ttgcggatcg acacgttccg gtccggcggc
    20641 aaaggcgggc agcacgtcaa caagacggat tccgccgtcc gcattaccca tctgccgacc
    20701 ggcattgtcg tgcagtgcca gaatgaacga tcgcagcaca aaaataaatc gatggcgatg
    20761 aaatatctga aatcccggct gtatgaaagg gagcttcagc agaaaaatga aaagctcgat
    20821 gaagaaaata aacagaaaaa agacatcgcc tggggcagcc agatccgctc ctatgttttg
    20881 catccctata agctggtgaa ggatcatcgc accaacctgg aaatcggcaa cgcccagaaa
    20941 gtgctggacg gggagatcga tgattttatt caggcctacc tgatggccgt ttcgaatcgc
    21001 aaatcctgaa ccggttattc cccgccggta aatcaggagt aacatttgcg attgtcgata
    21061 tcgtttgggg atgcgggcaa aatgtgttat aaagcccgcg aaaaaccggg aggcgccgga
    21121 atccggatgc cccgcgcttt ttcgttcagg gaaacaaata aaaacgcgct ttgaaaacga
    21181 caggagagcc ttgcccatgc gaaaatataa aaaatacaat ttgtcccgtg gtctgttgat
    21241 tttcctcttc atgatcgtgg gttttggctt cgcgcccgcg gacagagccg aggctcagct
    21301 cccggaggtc agtgtcccgt ttcagaatcc gcagtccgtt tcatcgccgg aatctcaggc
    21361 gggccctccc gcaaatccgg ccgtccggga agaccccaac gcggcggagg aagactctgc
    21421 ggacttggaa gaagagcagg atctgatgga aaacgtgctg gccattctgg aagtggcgga
    21481 caactactgg aagaacggcg atatcgaaaa caccctcaat gaactggata aggcctatgc
    21541 gctgcttctg gacacgaatg gcgatgtgga aatcgcccgg cagaaagatg acctgcgcct
    21601 cctgatttca cagcggatta tagcggttta ctcgtccaaa aaaggcccca ccaacggcaa
    21661 ggccagtgag attcaactca tcatgaaccc ggatgtggag aaggaaatcc gctccttcca
    21721 gggccccgaa agggaatttt ttacatcctc ctatctgcgt tccggtctat acagggagct
    21781 catcgtttcc gaactgaaaa aagcgggcgt ccccgaggaa ctcttctggc ttcccctggt
    21841 ggaaagcgga ttcaaggtca gcgcgtattc ccccgcgagg gctttgggac tgtggcagtt
    21901 tattccgtcc acgggttaca aattcgggtt gacgcgggac gaatgggtgg atgaacgcat
    21961 ggacgtcctg aaatccacgc gggcggccat tgcctattta aaggagctgc acgcgatgtt
    22021 cggcgactgg ctgaccgtac tggccgccta caactgcggc gaaggccggg ttctgcgcgt
    22081 catctccagt cagcgcatca attacttcga tcgtttctgg gacctgtatt cccggctgcc
    22141 ccacgagacg gcccgctacg ttccccgttt tctcgcgacc cttcatattg tcagaaaccc
    22201 tgccaaatac ggtttcgagc tgcagacttc gacggacacg ctgatgaatt atgagacggt
    22261 gaaggtcggc aagatgatga agctgtccga tgtcgccgcc aaaatggaag tgtccgagga
    22321 ctacatcaac gtgctcaacg cggagcttcg gcataaaatc acgccggacc gggaatatgc
    22381 cttcaagatt cccgaaggat cccagcagaa atttcaactg gcgtacaatg atattccggc
    22441 cgcggagaag cccggcaacc attacgggag cgacacgggc aaaagcagca aggcgggcgc
    22501 cggccgctat attcggcacc gggtccggcc cggtgaaacg gtggcctcca ttgccaagcg
    22561 ctaccgggtt tccagacagg cgatttacag ctgcaaccgg atcaacaaac gcaaaagttt
    22621 gagccggcag acccggtatg tccgcatccc gattcacatg gcggaatcgc ccgccgtccg
    22681 ccagaaagcc tccggcggaa aatcctatgc ggcgtccggc tccatgaaga cctacaaagt
    22741 caagaagggc gatacgctgt ttcgcatcgc aacacgattt aacgtacccg tcgcgaaact
    22801 gaaacaggcg aatcgtctga aaggcaacgc catcaaatac ggaatgacgc tgaaaattcc
    22861 cgatcaggga tgacatccgc cggaccggat gtcggaatgc gtcagcagca ggaaaagctc
    22921 ctgttctctt tttttcatct tcagggcgtt ggcccgggat gtccggacat ggtcgtagcc
    22981 ggtcaggtgc agcaggccgt gaatgatcag aaagagaagc tcctcctcca gagtcaaaga
    23041 acccttccgt gcgtcacgca gcgccgtgtc cgcggaaatc acgatatctc cgagcaggcc
    23101 tggattcacc atgccgcact cgccttcctt aagtgaaaaa gagagcacat tggtcggttt
    23161 gtcgaggcga agatactgcc ggttgagaag gcggatggcc tcgtcatcca caaaagtgat
    23221 gctgatttct ttttccgcgc agtcgatcaa acgcaaaagc cgggccaccc ggccgcggat
    23281 ccggcggtta tcgattttca accgcttctg caggtttgcg attcggattt tcatgggatt
    23341 ctcttttgcc gttcgtttcc ttcggcttta cgggcgcttc cggatattca acgcgatgat
    23401 ggaaaatgcc ggttaaaatc cgcgtgaagg tttccgcgat ggtgttcagg ttgctaagcg
    23461 tcagctcgca ctcatcgagc tgtccgtccg tgtaaatgcg ggcgattctc tccctcacca
    23521 gcgaacggat tcgggccggc gtcgggttgg aaagggtgcg agaggaagct tcaatcacgt
    23581 cgcccagcat gacgagcccc gcttcctttg tctgcggcct ggggccggga tagcggaaat
    23641 cgctttccgt cagcgagcgg atggattcat ccttgtcttt tttggctttg tcgaagaaat
    23701 agctgatcag gctggtgccg tggtgctccc ggatgatgtc gatgatcggc ttgcccagct
    23761 tggcctgcag ggccagttcg cagccttcct tgacgtggga aatgatgatc agggcgctca
    23821 ttttcggaga aagcctgtcg tggcgattgt catagccggt ctggttttca atgtagtaat
    23881 gcggtttggc cagcttgccg atgtcgtggt aataggcgct gaccttggcc agaagcgcgt
    23941 tggcgccgat ggcctctgcc gccgattcca ccagggaggc gacaatgatg ctgtggtggt
    24001 aggtgcccgg cgcttcaatg atcattttct gaaaaagggg ctggttgaga ttggccagct
    24061 ccaggagctt gatgtacgtg atgaatccga agaggctttc aaaaacaggg gtaaaaccgg
    24121 ccaccaggat tccggtgatc agcccgccga agaggcccat ggccagacgc agaaccagat
    24181 cctccagcag gcgtccggtc agcagattca ggctgagaat ggcggcaatg ttgacgacgc
    24241 cgagaaagat gccgaccttt aaaaaagtgg agcgctgacg gctgttgacg atatggtagg
    24301 aggcggcaac cgaccccaga aacgagaaaa gcggaaaaag aattttttct tcaaaaagca
    24361 gactgatcag aaacgaggtt aaaacgctca tgatcatggc cacgttccgg ttcaccagaa
    24421 cggcgataat catggcgccc atggcgaagg gaatggcgaa atagcaggcg tccaccggaa
    24481 gggaagggaa agcccggttg acggcgaccg aaatgaaaat tcccgctttc acgacaaaga
    24541 tttgcaggag ggcgacgatg gcgaacacca ggaagtccac gttggaccgg ggcgatgttt
    24601 tcagccagtt ccgggtgcgc cagaaataca gaagcagggt caaaaacagg acaatgcaga
    24661 aaatgcccgc gatgaccgcg agattggaga tgttgttgtc cgcggcggtc ttgtgaaggg
    24721 cctccagctt ggccagctcc agatagccga ttttatcccc ttcccgcaca atcatctcgt
    24781 ttttctgcac ctggaaatac accggcttga cggcctcttt cgcggaggcg gttttttggg
    24841 ccgttgcttc cgggctgcgg atgagattgg gcgcgatcat ttttttcaaa aaagaaaacg
    24901 acgcccgtct ggcgtcgggc tggtcgctcc ggatcgaggc atgaaccttc ctgagcagcg
    24961 ccggaccggt gtctttcatg tgcagaatgg acgacacctc tttggtgttt tcctcctgcc
    25021 tcgtcagggt gttgacaatg gtaatgcctt tttcccgttc atgattctgg aaggcgcttg
    25081 cggtgacgag cgtcttgtcg taaaaggcga aaatcacccg ggataatttc tgcagcagtt
    25141 catcggaaaa tgcgttggct ttcaggaagg cgtattcctc ctgcgtgagc gcgacgccca
    25201 gggagttttc cagaagagtt ttcccgtcgg gtttctccgg ctgtccggcc gcctgcgcga
    25261 aggattgcct gattttcgcc ctgatcgtca tggcggtttc cggatcatat tcatagaccg
    25321 gacgaacgcc ggccgcggct tcggtttgct tcagccttgt ggattgaatg tcctccacca
    25381 ggaattcgcg gtcggccttg atgttctcct gcacaatcat gccgtggcgg taaaccggtt
    25441 tggaaacgta gaattgcgga gcgagaaggg ccgtcaggcc cgcgctcaaa aggatggcaa
    25501 tcgcccatcg ctggaaaatg acatccttca ggaaagaaaa agagaaggga tgcttttcct
    25561 tccatttccg aaaaaaggac aggatctttt ccgatgtcga atccaggaaa ttgttcatgg
    25621 ccacgttcaa cttttttggt ttttgttttc tttttcccag gatgcgtagg cggagatcac
    25681 atcggccacc agggggtggc ggacgacgtc cacctgcgaa aaatgaacaa tgcggacggc
    25741 cggtatattt ctgagaacgg ccgccgcctc gaccaggccg gaggatctct ccgtcggcag
    25801 atcaatctgg gtgatgtcgc cggtgacgac cgctttggag caaaacccca gccgcgtgag
    25861 aaacattttc atctgttccg acgtggtgtt ctgggcttcg tccaggatga tgaaagagtc
    25921 gttcagagtc cggccgcgca taaacgcgag cggcgccacc tcgatgagcc ccttgccgat
    25981 cagcgcgttc gctttttcca tgtccaccat gtcatagagc gcgtcataca gggggcgcag
    26041 gtaggggttc actttttcgg ccagatcgcc cggcaggaaa cccagccgct cacccgcttc
    26101 cacggcgggg cgggtcagaa tgatccgctg gacctttctt tccagaaggc aggacacggc
    26161 catcgccatg gccaggtatg tttttcccgt gccggcgggt ccgatggcca gcaccatgtc
    26221 ggcgtttctc atggaatcaa tgtagcggcg ctgggcgacg cttttcggcg cgatgacccg
    26281 ttttttggag gagatgaaaa ccgtgtccag aaatacatgg gacaggtcaa aatcaatgtc
    26341 ctgggaaaga atccggtggg cgtagtcaaa atccgagagc tcgacgtgca accctttgag
    26401 catcaggtcg cagagctgcc ggatgacgcc cgacacaacc gcgacggccg cctcttcgcc
    26461 ggaaatagaa agcgtgttcc cccagggctt gattttgacg tcttcaagct tttcgagaag
    26521 cttcaggtgc cggtcgtgtt cgccgcacag atttcgaagc agattcgagt ccgcgaagga
    26581 aatcttttcc gctctcgcgc ttttcgttga ttttgtcaaa aaaatccgcc tctaaaaggt
    26641 tgatctccgc cattgtccaa ccgttttacc ggtgatcttt gcgatggcca tggccccgtt
    26701 gaacgaggag gctatctatc atcaagataa cggcattgca atacaattta agcctgcaaa
    26761 aacagatggg cgaagacctt tgcgttgccc gtcatgttgt ccaccgggca gttttcgtcc
    26821 ggctgatggg ctttcatgtt ggttttggac cagacggccg ccggcaggcc ccgtttcctc
    26881 agataggcgg cgacggttcc cgcaccgacg ccgccggccg cggcccgaac gcggtaaacc
    26941 cggtcgacgg cgttttgcag ggcgaggacg acgggcgcgt cgtccggcgt ggaaggagcg
    27001 gggtcaacgc gctgatgaat ggaaatttca atcgttacgc catggttttt ttcgacttcc
    27061 cgcttgatgg cttcaatttc aaaaagcacg tcgtcgagtt tgtaccgggg caggattcgg
    27121 caatccatgc agaagacgtc ttctcccgga atcgtgttga tgttgccgac gttggcttcc
    27181 ttcttcgtgg gttcgaaggt gctgacggga ggatcaaaaa gaggatcttg tgcgtcgaag
    27241 atttcgcgca gctttgtcag ttgcgtcacg aaggaagagg cggcggaaaa agcgttgatt
    27301 ccgagctgcg gtttgctgcc gtggcactgc tttccggtgg ttttaaagcc cagccagaga
    27361 atgccttttt cggcgacttc aatcagtgac ccttcggaat ctcctgaatc cggcgccaca
    27421 atccagtccc cggaaccgaa aatcttttcc ggcagggcga ggagatgctg cacgcctttt
    27481 tcgctggagg tttcctcgtc ggatacgaac agcaggttga tcgtggagcg gggaacgatg
    27541 ccctccgcaa gcagcgccct ggcggcaaaa atggaagccg ccatatcctg ctgattgtct
    27601 tccgtgcccc ggccgtacac gcaatcgtcc tttacatagg catggtaggg atcggcgctc
    27661 cagaggcccg gctcgcccgg agggacgatg tccagatgcg taatgatcca gacgctgcgg
    27721 gcgttgttcg ttcccggaat ggacgcaaga aagttcgggc gcagtccgtc cgaaaccgag
    27781 ggatccggcg cgtcaaaatg ccggaaaatc gtaaagccca tatcggacag atgcttttgc
    27841 agcgtccggg ctttgggcat ttcgccgtcg ccgccgtttg ccggaccgac ggccggaacg
    27901 gccgtcagcg ctttttgcag gtcgatcatg tccccgcgcc aggcatcaat tctgcgggat
    27961 actttttcaa aaagaacttc gtcgatcatc atcaaacctc aaatcagggc tggggaacaa
    28021 tctgcgccgg cgccggccag ttttccggcc atgcgccgaa acggtccgcg tcgccgacga
    28081 tcagcgtcaa tcttctattt tcatggagaa aggccgcggc gatgcggcga atgtcttcca
    28141 gcgtcaccgc ttcgattttt tcccggtagg tcgtcaggta atcggcgggc agcttctcgt
    28201 attcgttttt catttgctcg aaggcaatct gtcttggctg ttcaaaggaa aagatgaagc
    28261 tgttgatgat cgatcgcttc gcccagtcca gttcggcccg agcaatggac ccggccgccg
    28321 cgtcgcgcag aatggattcg atcagggaaa tcgcctgaaa ggtcgaagcc gtcttggtga
    28381 aggcgtaggt tccgaaaaca ccgtagttgg accgcgcgcg gtagaagctg ccggcgctgt
    28441 aggccaggcc ttcctcattg cgcacggcgc tgaaaatccg ggaggaaaat ccgccgctgc
    28501 cgatcagaaa atccagaacc tcgaaagcgt aaaaatccgg gtggttcttg ctgatcgtca
    28561 gttccccgcc gaccaccgtg gattgcggga tcggtttgcg gatcaggaaa aagccccggc
    28621 ctccctgtcg gggggcggcc ggaatgtcat gaacgaggcg gggaatcttc gcatcgccga
    28681 aaacttgttc gatttttttt acggcttctt cacgggagat gtcaccggat accgccagca
    28741 gcatgttggc cggcgaaaag tattcgcggt ggaacgaaat cagatccttg cgcgaaatgt
    28801 tgtccaggga ggcggaggtc gcgtagcggc cccgcgggtc gccgggatag agaagccggt
    28861 tgaactcgcg aaaggccagt gtttgcggat tgtccaccgt ccgccgcagt tcctctttct
    28921 ttaatgccag cgcctcctga atcttttttt cttcaaaggc aggctccaga agcatccggg
    28981 agagaagatc gaggcattca tccaggtcgc ttttcaaaaa gatgaagtga atggaggcgg
    29041 aatcgagcga catggaaatg gacggagaag cggccagaaa atccaggcgc cggtccattt
    29101 cgccgcttcc gagttttgcc gttccgcctg tgcgcatcac cgaggcggta atttccgcaa
    29161 gtccttcttt tcccgcgggg tcgtgcaaag ttcccgtccg caccagtgcc gtcagggaaa
    29221 ccagcggcaa ttcgctgtcc tgcagataaa acaaaaacat gccgttttta agcgcaatcc
    29281 gctgggcctg cggcatgcgg aattccagcg cgggataccg gatttgatcc ggaggaatcc
    29341 aggggggtgc ggtgtgtgcg gcctggggcg ctgcaagaaa gatcaatgcg agcagcagtc
    29401 cggctgtgca aaatatcgtt tttttacttt tcatgggttt ccgcgccttt tctggaagcg
    29461 ggcttccggt ttaaaaacgc gacggtgcgg ttttccgccg taaggtaccg gacggccgcc
    29521 gcccgaacct cgccggccgt caccttttgt attctgggca ggtaatcgga aaagtaccgg
    29581 taatccccca aaagcagttc gtaataggaa agaatggacg ccagttcgga attggaatcc
    29641 aggcttttga tgtagtccat cttcagctgg tttttggccc gggccagttc ctcttccgtc
    29701 acggaatggg tcttgatcga ttcgatctcc tcaataatgg ccgcctgaag ttcttcgtta
    29761 gtgtgggggt ggcgcggccg cgcaaaaatc gtgaagaggt tggagtatct cgaggcgggc
    29821 acgccgttca ccacgctgac actgtccgcg atctgccgct ctgtcaccag cttgtcgtac
    29881 agacggctgg ttctgccttt gccgagaatc gcctccagga catcgagcac ataatcatcc
    29941 tgcgcgggcg gcgccggttt gtggtagccg atgatcatcg acgggcttgc gtcgaactca
    30001 acctcgacct ttcgctcggc tctttgctcc ggttcggaag gaatccgcgc ggaaggcgcg
    30061 ccggcatcgg gaatccgccc gaaataacgg cgaatcaggt ccatcgtttt tgaagggtcg
    30121 acatccccga ccacggcaat gcagatccgc ccgggtgacc ggtatttccg gtggatgccg
    30181 cggatcgccg ccgggctcag gaaggtcaga tccatgggca atcctatgat cggaatgccg
    30241 tagggatggg tcgtgtaggc ccggcccaaa aaggcttcat agagcttgcc gtcggggctt
    30301 gcctccactc gctgccttct ttcctccagg atgacgtccc gctccgtgta aaactgacgg
    30361 aaaacgggat gcagaaggcg gtcggattca atgcgcgccc agagttcgat cttgttgg
//
LOCUS       DAOPAF010000015        30330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1653_length_30330_cov_58.254170, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000015 DAOPAF010000000
VERSION     DAOPAF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 30330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30330
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_30330_cov_58.254170"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLQ77_02935"
     CDS             <1..747
                     /locus_tag="PLQ77_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL09665.1"
                     /translation="TEQGSVEVKVNTEKASFGGEKDDLCLVHISVKDTGIGIPPEKQG
                     MIFERFTQADSSVTRKYGGTGLGLSISRRLAELMGGRLWVESTPGQGSTFHFTLLLRI
                     EDKTRQAAPPAKSEKAAVPEQRPLKILLVDDSDDNRLLVKTYLKGDAYQIDEAENGEQ
                     AVALFQSGLYDLVLMDMQMPVMDGYTATRAIRRWEEEQGKNRTPVIALTAYALTEDAA
                     KSEEAGCDTHLTKPVKKAVLLEAIRRFAAP"
     gene            918..2582
                     /gene="argS"
                     /locus_tag="PLQ77_02940"
     CDS             918..2582
                     /gene="argS"
                     /locus_tag="PLQ77_02940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPL09666.1"
                     /translation="MKKKLISLIARSVGACAGKGLLPDVDLSRIEVEVPANPEHGDYA
                     SNIAMVLASQARQNPRKIAQAIQENLDDPEGIVEKTQIAGPGFLNFMLKDDVWRSVLK
                     DILDQKDRYGCLPAGSGKKVQVEFVSANPTGPLHIGHARGAVVGDVLCNLLAATGQQV
                     SREYYINDAGNQMNNLGRSVLYRLRELAGEAVEFPENGYRGDYIREIAGDIFKKEGSR
                     FRDENEEETIQRFNEAAGAVILDGIRKDLRDFGVTFDCYFSERELYRENGVGRLLDEL
                     QKQGFIYSDGETLWFKTTDYGDDKDRVVIRKNGEPTYFAADIAYHANKFSRGFDLLID
                     IWGADHHGYIPRLEAAIQAIGRPREDLKIILVQLVNLLRAGEPVAMSTRSGEFVTMRE
                     VLDEVGRDAARYNFLMRRSDSHLDFDLEVAKKQSNENPVYYVQYAHARICSILRTAQE
                     RGMTLPDFPAIRPSLLAEAEERTLIKMMARYPEMLEGAAKTLEVHRITFYLNELAAVF
                     HGYYNKYKVVTQDAALSNARLVLVAAVREVLKNALGILGVGAPEKM"
     gene            2593..3354
                     /locus_tag="PLQ77_02945"
     CDS             2593..3354
                     /locus_tag="PLQ77_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPL09667.1"
                     /translation="MGSGNTRKFEIRLGQTGLTVLIVGMVVLLCLSFLFGVGVGKNID
                     TYPEKIASLPQRFLALFWKPAKVAVEQKSVPVAQAGKANVDLAFHQALTSSKTVPIQP
                     LPDAEKTAEVEAFADREEPLPPPSSPSAQKEKAAPLKPEPVSPKKAAGEKPAAEIKSK
                     ESKTDSAAASYQVFVASLKDRQKADQIHRIVARAGYASKIVKVDLKGKGTWYRVIATG
                     FDTRAKAEAAADKLSKRAKTKCIVRPTAAGKAANP"
     gene            3424..4752
                     /gene="ffh"
                     /locus_tag="PLQ77_02950"
     CDS             3424..4752
                     /gene="ffh"
                     /locus_tag="PLQ77_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPL09668.1"
                     /translation="MFESLSEKLEGIFKKLKGRGVLNEESVNAALREIRMALLEADVN
                     FKVVKDFIEDVRGRAVGKEVLESITPGQQVVKIVHDRLVELMGSTSAAIKFGPRIPAP
                     IMLVGLQGCGKTTTAAKLARLISADKRVYLVSADVYRPAAMEQLAVLGRQVKAGVFEA
                     GSCKDPVKICVDAVEQARREGYEVILIDTAGRLHIDEELMDELRRIREKVNPAEILYV
                     ADAMTGQDAVNVGAKFNELIGIDGVIMTKMDGDARGGAALSLRSVVGKPIKFVGVGEK
                     IDALEVFHPERMAGRILGMGDILSLVEKAQATIDEKTAKQMQESLRKSDFTLEDFRKQ
                     LGQIKKMGPLKDMIGMIPGMGKIKALKDMEPDDGELVKIAAMIDSMTKKERNNYLIID
                     GRRRKRIALGSGTTVQDVNRMLKNYIELRKMMKKINSKGGMKALMRNFPF"
     gene            4817..5071
                     /gene="rpsP"
                     /locus_tag="PLQ77_02955"
     CDS             4817..5071
                     /gene="rpsP"
                     /locus_tag="PLQ77_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPL09669.1"
                     /translation="MAVKIRLTRMGAKGKPFYRIVAADKEYPRDGRFLEILGNYDPKK
                     NPAEIKLKEDRVRDWLSKGAKPTLTVSHLLKTKGIVAAVK"
     gene            5199..5429
                     /locus_tag="PLQ77_02960"
     CDS             5199..5429
                     /locus_tag="PLQ77_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPL09670.1"
                     /translation="MKDLIKYISQALVDNPDQVEVTEVIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRTILSATSAKVHKRAVLEIIE"
     gene            5431..5937
                     /gene="rimM"
                     /locus_tag="PLQ77_02965"
     CDS             5431..5937
                     /gene="rimM"
                     /locus_tag="PLQ77_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPL09671.1"
                     /translation="MDLFVFGEIVKTRGLHGCLKALSFLESSELLDGLTDVYLETPSG
                     RKSRHEIKKINSSGKFLFLEIKDVSGVDEARRLVGSRLLMPRDRLAALAADEYYWHDI
                     VGLDVYTPRGRHLGRIESVFPTGGNDVYVCRGDAGEILIPATAEVVLQVDLAGKKMII
                     QPLEGLLP"
     gene            5934..6671
                     /gene="trmD"
                     /locus_tag="PLQ77_02970"
     CDS             5934..6671
                     /gene="trmD"
                     /locus_tag="PLQ77_02970"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPL09672.1"
                     /translation="MIRFDVLSIFPEMLQSPLEFSLLKKARDKGLVEVGLHNIRDWAE
                     DKHRMTDDAPYGGGCGMVMKVEPVARALAAVKRPVGPSLTVLMTPQGEIFQQKIAAEL
                     ASQEQIILICGRYEGFDERIREHLADREISLGDYILTGGELSALVVIDAVSRLIPGVL
                     GNEDSASRESFSQGLLEYPQYTRPASFSGWSVPEVLVSGNHAQIERWQRIEALRRTWQ
                     RRPELLEKAGLGGEDEELLEKIKLGRI"
     gene            6762..7109
                     /gene="rplS"
                     /locus_tag="PLQ77_02975"
     CDS             6762..7109
                     /gene="rplS"
                     /locus_tag="PLQ77_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPL09673.1"
                     /translation="MNVLEMIEKEQMRGDIPAFKSGDTVKVYVRITEGQKQRIQAFEG
                     VVIRKRRGNSRANFTVRKVSYGIGVERTFPIHSPVIDRIEVVSRGLVRRSRLYYLRSL
                     RGKKARIKEDTGH"
     gene            7129..7752
                     /locus_tag="PLQ77_02980"
     CDS             7129..7752
                     /locus_tag="PLQ77_02980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPL09674.1"
                     /translation="MNTFERLAQVEGYKTIAGVDEAGRGPLAGPVVAAAVIFPPEYSN
                     PAINDSKKLTAAKREDLYRVITREAMAVGLGVVEADVIDRINILQASLQAMREAVTEL
                     SVRPDYLLIDGLNSIDFDAPQKAIVKGDSLSVSIAAASIIAKVSRDRIMEMYHRQFPR
                     YNFLKNKGYGTVGHRRVLEEIGMCKIHRRSFHLKKQNLPQTALDWSE"
     gene            7749..8135
                     /locus_tag="PLQ77_02985"
     CDS             7749..8135
                     /locus_tag="PLQ77_02985"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPL09675.1"
                     /translation="MKDSQDRRKIITGRAGEAMAADFLKRRGYRILEMNYRCSIGEID
                     MVAREKDVLVFVEVKTRKSGAMGYPEQAVGLVKQKKMSQLALWYLRDKNIGDAAVRFD
                     VVAILLLPSGNDIRLIRGAFDFIALE"
     gene            complement(8183..9559)
                     /locus_tag="PLQ77_02990"
     CDS             complement(8183..9559)
                     /locus_tag="PLQ77_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPL09676.1"
                     /translation="MKFKKKILGIGAGYVGAPSMVMIASKCPDYKVTVVDINPERIAA
                     WNSDRLPVYEPGLDAIVRKTRGRNLFFSTDVEGQIQENDIIFVCVNTPTKTFGAGAGM
                     AADLQYWEKTARQILENSKTPKIVIEKSTLPIKTAQAMERILSSGKKNVTFDVLSNPE
                     FMAEGTAVSDLENPDRVLIGSRETRSGLAARAALVEIYTRWLPREKIITSNIWSAELS
                     KLVANAFLAQRVSSINSISALCEKADADISEVAHAVGTDSRIGAKFLKAGIGFGGSCF
                     KKDILNLVYLCRFYGLPEVASYWESVVRINEYQQDRFIANILRSMFNTLAGKKICVLG
                     FAFKADTGDTRESPAIAITRKLLAERAEVIITDPRALANAAIDLAHEKGNIRYVEDPY
                     EAARGCHALAVMTDWEEYRKLDYRKIYKSMVKPAFLFDGRNIVDHRKCFEIGFNVFPI
                     GKPALTRF"
     gene            complement(9585..10526)
                     /locus_tag="PLQ77_02995"
     CDS             complement(9585..10526)
                     /locus_tag="PLQ77_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL09677.1"
                     /translation="MKRILITGGAGFLGSHLCERLCGEGHDVICLDNFFTGSKNNISH
                     LLGQTHFELIRHDIINPIYLEVDEIYNLACPASPVHYQFNPIKTIKTNVMGAINVLGI
                     AKRTRAKILQASTSEVYGDPEVHPQHESYWGRVNPIGIRSCYDEGKRAAECLMMDYRR
                     QNAVRTTIARIFNTYGPRMAPNDGRVVSNFILQALQNRDITVYGDGSHTRSFCYAGDL
                     IDGLIRLMNTPDDFFGPVNIGNPGEFTILQLARLVIELTGSTSQIVFQPLPSDDPAQR
                     KPDIALAREKLGWQPTISLEKGLIGTIAYFKEILKTA"
     gene            10594..11481
                     /gene="rsmI"
                     /locus_tag="PLQ77_03000"
     CDS             10594..11481
                     /gene="rsmI"
                     /locus_tag="PLQ77_03000"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPL09678.1"
                     /translation="MSTIKSQRGGKMDREARGVLYMVATPIGNLEDITLRALRVLKEV
                     DLVAAEDTRRSKILLSAHGIKTPLRSLHEHNEKERSAEILSRLQNGKSVAYVSDAGTP
                     CVSDPGRHLVRAARDNGIRVVPVPGACALPAALSVCGFQAEPFLFCGFLPPRSNRRRR
                     FLESIMEEECSIIFYESPARIRETLEDALDILGDRQVMAARELTKVFEEIREGKISEL
                     LAAWPGAGVKGEFTVMLQGAAREPAVLSEREIGEQLLKILSEKGVSLRDAVAEAARRT
                     QMPRKKIYDAALRIRREFP"
     gene            11663..12259
                     /locus_tag="PLQ77_03005"
     CDS             11663..12259
                     /locus_tag="PLQ77_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HPL09679.1"
                     /translation="MKIRPVFFALIIALALCLCACSSRGPDTLKNDAGTKAVNRIALL
                     PVENQTGDARVPKMLRLKLSEELRFKGYPQVDIEVIDGRLAAMAGAGEPGKTAIAPGV
                     VREMLGADAAMYCSLMESKVSKKIFYTPVTVSVRCELRSTATGETLWNAEGKATGRSF
                     DLRSKTLDLKALGDLEAVLEKVVDEVMESLPYGPKLSG"
     gene            12325..13092
                     /locus_tag="PLQ77_03010"
     CDS             12325..13092
                     /locus_tag="PLQ77_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPL09680.1"
                     /translation="MREKTKIIVAVIMTLMLAAAAQAQSPVTVKMTKGRAQITSLKGK
                     VSMVCPGGKEAFYLKTYDFILPGCEAATGPDSRVEMVLPDRSVVRFSEKTKFKLVQAD
                     IGARGSRSVGISVTLGKIWTNVRKSLVGGSDRFEISCQNAVAGVRGTVYRMDVEKDQS
                     AVVKVYDGEVSVAGVSPAQPKAPVAFGPPKPVAGPTVIEGPKPVSMEQWVYIVRSMQQ
                     IQITVDGQAQKPRDFTQEEDMDDWVKWNQERDKQYGQ"
     gene            13114..13671
                     /locus_tag="PLQ77_03015"
     CDS             13114..13671
                     /locus_tag="PLQ77_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPL09681.1"
                     /translation="MNIPNFLSLLRIILVPVFVIFLIQAEYDRALITFVVAGLTDALD
                     GTLARLLKCQTVLGAYLDPLADKLLLATSFIMLAVIGLIPAWLAVVVISRDLIILLGI
                     AIFSLMSIAYEIKPAIISKVTTALQVGTIFFALLNKALPQDFGTFWIAVLFWATALFT
                     FGSGVVYILRGIRILNKSAPEETKK"
     gene            13732..13806
                     /locus_tag="PLQ77_03020"
     tRNA            13732..13806
                     /locus_tag="PLQ77_03020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13764..13766,aa:Val,seq:gac)
     gene            13915..15831
                     /gene="thrS"
                     /locus_tag="PLQ77_03025"
     CDS             13915..15831
                     /gene="thrS"
                     /locus_tag="PLQ77_03025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPL09682.1"
                     /translation="MMDIKVIFPDQSQQTQPAGVSVAEAIGSWKKEALATAVAASVNG
                     ASVDLNFALAEDAKVEPIDIASKKGLSVLRHSISHVMAQAVQDVFPGAKVTIGPSIED
                     GFYYDFEYAETFTVDDFEKIEKRMKEIVAADYPFVREELPREKAVDLFAGKGEDYKVE
                     LIRDLPADVSVVSLYKDGDYVDLCRGPHIPRTGMIKAFKLLSVAGAYWRGDERNKMLQ
                     RIYGTGFADEQALQDYLTLLEEAKKRDHRKLGRELDLFQVNDEAGAGLVIFHPKGMLL
                     RYLIEEWERKEHLKRGYDMVMGPQILKVDLWKKSGHFDHYRDNMYFTEVDEQTYGIKP
                     MNCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKTGVLHGLMRVRQFTQDDAHILCM
                     PEQLNAEIRAIADFVHYAMGIFGFEYEVELSTRPEHSIGSDADWDLATKALENALVDN
                     KIPYEVNEGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYIGPDGEKHR
                     PVMLHRVILGAIERFMGVLIEHYAGAFPVWLAPTQCVVLTVTDKQIPYAESVRGRLVE
                     AGIRCEPYFDNDKLGYKIRQAQMQKVPYMLVIGDKEVAACAVAPRARDGRNLGAMPVD
                     EFIALIQEACGSKK"
     gene            15887..16378
                     /gene="infC"
                     /locus_tag="PLQ77_03030"
     CDS             15887..16378
                     /gene="infC"
                     /locus_tag="PLQ77_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPL09683.1"
                     /translation="MNREIKSANVRVINEEGQPLGVIPLEEAMAQAERAALDLVEVSA
                     NADPPVCKIMDYGKYRYKQSKKIHDARKSQTVIHVKEIRLRPKTEAHDLQVKIKHIKK
                     FLEQRDKVKISMIFRGREIAFTEIGRKIMEQIKAALADECIMDQEPRLEGRSMVMIVS
                     PKK"
     gene            16409..16606
                     /gene="rpmI"
                     /locus_tag="PLQ77_03035"
     CDS             16409..16606
                     /gene="rpmI"
                     /locus_tag="PLQ77_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPL09684.1"
                     /translation="MPKMKTHRGAAKRFSVTAKGKVKRSKAFASHILTKKTTKRKRTL
                     RKAALVHSANEKAIKRLIPYL"
     gene            16655..17014
                     /gene="rplT"
                     /locus_tag="PLQ77_03040"
     CDS             16655..17014
                     /gene="rplT"
                     /locus_tag="PLQ77_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPL09685.1"
                     /translation="MARIKRGVTARKKRRKILKMAKGFFGARSRLLRTATEAVNKAMS
                     YAYRDRRGRKREFRALWIARINAAARQNQISYSRLMDGLKKANIGLDRKILAELAVND
                     PQNFARIVQMAKGEQAA"
     gene            17011..18027
                     /gene="pheS"
                     /locus_tag="PLQ77_03045"
     CDS             17011..18027
                     /gene="pheS"
                     /locus_tag="PLQ77_03045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPL09686.1"
                     /translation="MIADLQHLEKNALAELEKTGSEDSILAVRTKYLGRKGLLTGLLR
                     NISSVPAEERRSFGKRCNEVKELLEKNIEEALRRRTEDQKAGMLAGQQIDVTLPGRGI
                     RAGSLHPVVQIRREICDIFAAFGFSVVEGPEVELDYYNFEALNIPKDHPARDMQDTFY
                     IEENMVLRTHTSPVQVRIMEKVKPPVRILSPGRVYRRDSDVSHTPMFHQVEGLLVDRG
                     VSFGDLKGILTAFLRKVFGEDTILRFRPSFFPFTEPSAEVDIRCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFQNVGYDPEVYTGFAFGMGLERIAMLKYGISDIRLLFENDIRF
                     LKQF"
     gene            18042..20450
                     /gene="pheT"
                     /locus_tag="PLQ77_03050"
     CDS             18042..20450
                     /gene="pheT"
                     /locus_tag="PLQ77_03050"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPL09687.1"
                     /translation="MLVSLKWLKDYIDVETAPEELADRLTMAGLEVDEIRTLSPRFSG
                     VVVAKILSVRPHPNADKLSLCDVTDGAQTYPIVCGAKNIHPGDIVALAKIGAIIPGGY
                     TIKSTTLRGEKSEGMLCSEAELEIGDDASGIVQLPAGMTPGTPLEEALDLNDTVLDVG
                     VTPNRSDCLSMIGMAREAAALTGKTLKMPEGKVREAAEDVSLLSSVTIEDPDLCPRYT
                     ARLIRNVTIGDSPVWMKTRLEAAGLRAINNVVDVTNFVMLEMGQPLHAFDFHFLEERR
                     IVVRKSKPGEEFVSLDGKSRILPENTLLICDGKKPVAIAGIMGGLNSEVKEDTRTIFL
                     ESAYFNPSSIRRSSRRLAMPTDAAFRFERGIDPEGVIRALDRAAQLIADLSGGEVCAN
                     RLDEYPAKIKPVENILLRMDRAGQVIGVAVPAREAVRILKSIGMTLKREGRGVYRVTP
                     PSFRVDIEREIDLIEEIARLYGYDRVPSTLPSVSVTELDAIPRLALEERIRLLLTGFG
                     YSEIINYSFTTPFCAEALGLPPEDERRRFVVIRNPLTEDQSVMRTTLTYGLLETLKKN
                     INNASWNLKLFEIGRTFLRRGDGELPEERNILAALAAGKASDDLWGLKAGVDFYDLKG
                     CLENIFQDLKFDSMRFRAEKPQSYLHPGQSCGVYADDVRIGSMGKAHPDVLKKMDIKS
                     DAYVFEIDLDLLGAKIRPRVRCREISKFPAVLRDVAFVLPSSMESEKMLEMVLRQRED
                     LLENVSIFDIYAGKGLEEGTKSLGLRFSYRALDRTLTDAEVSAIHDRIVKDTIRLTGA
                     KIRA"
     gene            20484..20759
                     /locus_tag="PLQ77_03055"
     CDS             20484..20759
                     /locus_tag="PLQ77_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPL09688.1"
                     /translation="MTKIDIIQNVYEKLGFSKKESSDIVESVFDIIKDSLAAGERVKI
                     SGFGNFMVKEKRARRGRNPQTGEEISITARRVLTFKSSQVLRKSLNS"
     gene            20876..21214
                     /locus_tag="PLQ77_03060"
     CDS             20876..21214
                     /locus_tag="PLQ77_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPL09689.1"
                     /translation="MDGIIPDKAYFRIGEVSKILNVEPYVLRYWETEFRTVKPVRTSS
                     AQRLYRKKDVEELLIIRNLLYQQRFTINGAKKQLMKIRSGEEAPPEAASEDKLVLIKK
                     ELQRIKKIMS"
     gene            complement(21254..21532)
                     /locus_tag="PLQ77_03065"
     CDS             complement(21254..21532)
                     /locus_tag="PLQ77_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPL09690.1"
                     /translation="MLCETVKKGVECAFMSKKGCGFFGNSCQPVVDKCGECNKIFEFE
                     TGRYCKVYPDPKSKWLTGNCPTASHVKIEIKETQKTNPLKASKRANKK"
     gene            21773..22525
                     /locus_tag="PLQ77_03070"
     CDS             21773..22525
                     /locus_tag="PLQ77_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HPL09691.1"
                     /translation="MKKGSLLKIFWRSFFIHAALNFRRMQNLGFAYAMISLLRQRKMP
                     DREAEEMLVRHLQRFSTHPYLSAPLLGSILRMEEDRPDGEDAAPITAVKQSLMGPYAA
                     IGDAFFWGSLRPCAGICAVGLAWLGCVFAPLVFLLIYSPAHLLVRGRGFFEGYRGGKQ
                     GIAAIRAMDLPGVTGRIRWLALAALIAGSLLVFQAGFRGASAFGVAAAMLAALLLCWW
                     LVKRGVSQTYILYGAAALFMIFSTRGLWLCWI"
     gene            22522..22782
                     /locus_tag="PLQ77_03075"
     CDS             22522..22782
                     /locus_tag="PLQ77_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPL09692.1"
                     /translation="MKTFTLKNKLGMHARAAALFVRIAQKYRSDIVIERNGQTVNGKS
                     ILGILTLACPMGGQITVSARGDDADGALAELEALIENKFGEE"
     gene            22792..24576
                     /gene="ptsP"
                     /locus_tag="PLQ77_03080"
     CDS             22792..24576
                     /gene="ptsP"
                     /locus_tag="PLQ77_03080"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPL09693.1"
                     /translation="MMSDQGKKTFVLKGIGVSPGVVIGKVYRFDPLDAQISFYKLHSE
                     DMVPREIERFKNALKESSRQLLEIQENLKKTKVTEPLYIIDVHILLLSDKKFISRTIK
                     YIRRMGVNAEWALRMTLDHYKQIFEGLEDVYISGRISDVQYVIQRILRNLSGEKHEIV
                     WDVGQEGVVIVSHDLSPADTAQMKMDKIVGFATDSGGRTSHTAIVARSMELPAVVGLD
                     NVTRFVRTGDDIIVDGTSGLVVVNPYPDMLKRYEEKKRHYDAAKDEYLKYARKPAVTV
                     DGYSVKIGSNIEFVEEIPSAVSHGAEHVGLYRTEFLYIYRDELPTEEDHFNNYRQVVE
                     EKNFSWATIRTFDLGGDKFTKYQKHARELNPQMGLRAIRFCLKEIEIFKTQLRAIWRV
                     SALGRVKILFPMISGMEEIREAKRLLEETRRELLAQGAPIADRMEIGAMIEVPAAAVI
                     ADQLATEVDYFSIGTNDLIQYALAIDRSNERVTYLYEPLHPAVLRLIKRIVDSAHKAG
                     IRVAMCGEMAGDPLCCPILLGMELDELSMNHLAIPRIKRILQQSSLEESRKLLEKALS
                     YNNAAEVRACVQDYMTRRFPDEIQKREL"
     gene            24624..27245
                     /locus_tag="PLQ77_03085"
     CDS             24624..27245
                     /locus_tag="PLQ77_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPL09694.1"
                     /translation="MSEKTPTETGRPEEKYYSGCMYADQDLWRFHAAKAYLSRVSIAD
                     EDVQALKDLAGKGAVVYAIKQRSKLNSLILAEVTARKGLPMPVYSHGVNMSFWQPLSK
                     MMKFLWSSFLRRFRKNEIARQGKLEFLAGKVAARESVIIHLGESEFIESRASEDAIAG
                     LMNLQAGLPFPIYIVPVLVAYGRRREKEDESLFNILFGQTEHTGTMRRLITFIRYSKQ
                     AFILPAEPVHLPEYLSANSLLTKNELVMDLRGELIDRIEEEKTSVVGPALKSREAMMG
                     MVMHDPKLNGFLDEYARNNKKDRAGLARDVRRYLNEIASDYRETFVELWDKVLTWLWN
                     TVYDGVSIDSEGLANVRNISKKMPFVIISCHRSHIDYLLLSYVFYKNNIQLPFIAAGN
                     NLSFFPLGYIFRRSGAFFLRRSFRGNEVYGEVFSKYMAMLLQEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMIIQAYQDKYCENLAAVPVYIGYDRVIEEKSYLQELSGAPKVQENT
                     VEIIKSTKILRKRYGRVYVNIGQPIILKDYLAAQEKSMDEMTVEERQSLYRKIGYEVV
                     LEINKVSVVTPFALVASGLLSHDRRGISHEELRGILNEFYEYLSTRQVKFADTFAHRD
                     RAITDALNIFDETDIISKIEAEEEEEEMQEVVYSLTDARRLNLEYYKNNILHFFIPLC
                     FVATSMVKNPEDSMSLAKIMGDYRFLKGLLWNEFIFDENREDADDVTEALTYLHSKSM
                     ILTSRRDGEAWVEIKSKGEVRLKPFAGLIHNYLESYWIVIRSLVYLKKKPLSQKEWLA
                     KIRSLGDRMFRKGEVLRAEALSQANYQNAILFLESAEMIVSQAGEEKSGKRETVYSLA
                     NNRAQLEVLRRRLFKFL"
     gene            27357..28214
                     /locus_tag="PLQ77_03090"
     CDS             27357..28214
                     /locus_tag="PLQ77_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPL09695.1"
                     /translation="MSKRMKIVIAFVLIMQMVIPYPVYAATVGRFTSVVGNVILTRDG
                     KDYKPVVNSAVKEKDILVTGANSSAVMTFIDDSTIALSPKTRMQVREFGVEGKARKGI
                     FSLSIGKLVANVTKFIGGKNKFEVHSPTAVCGVRGTFFGVEVAMAGTVMTTTATCTTG
                     ALSVSALSATGAIMATSGIVAGQTAVITSTGITVGAAGAAAGAGAAAGTAGTTGAAGT
                     GAAAGTAGAGAAGAGAAGAAAGAGAAAGIGAGTIAAAAVVAAAAVAAVVATTGTTDST
                     PTHTTPAHP"
     gene            28306..29055
                     /locus_tag="PLQ77_03095"
     CDS             28306..29055
                     /locus_tag="PLQ77_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09696.1"
                     /translation="MTKKDSKLFLHLKKWLEKAVLDYGMIEEGDRVLVGVSGGADSFA
                     LLDLLGSPMIFVPQFSWTAVYIDMGFDPGFVAYRLLEEYFQTHQHPYVMEKTDIGTIS
                     HSDFNRKNPCFLCSRLRRKRIFEIADEKGCNKIAFAHHKDDIIETLLINLFYGREIST
                     MMPNQPIFDGKLHIIRPLAYLSEALVKKYSRERQFPAVKNACPTSGNSRRSYIKKLLD
                     QLEIDNPDIRHNIYTAMSHVKPDYLMPGIKK"
     gene            29159..29635
                     /gene="smpB"
                     /locus_tag="PLQ77_03100"
     CDS             29159..29635
                     /gene="smpB"
                     /locus_tag="PLQ77_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPL09697.1"
                     /translation="MSKDNAAHKLIASNKFARVNYDIDDVYEAGMVLSGTEVKALRAG
                     KANLKDSYAVVKDSEVYLRDMHISHYDFGNRANHEPLRPRKLLLHKREIRKLYGKTRE
                     KGLALVPLRLYFKNGMVKVEIGVGKGKKMHDRREDIKQREERRSIARDFKSKRIRV"
     gene            29715..30203
                     /locus_tag="PLQ77_03105"
     CDS             29715..30203
                     /locus_tag="PLQ77_03105"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPL09698.1"
                     /translation="MKGKKVSESRVMMGQGMNPENANPAGNVHGGDIMKLIDTAGGIA
                     ATRHARAHVVTASIDRMDFHHPVYIGDFLILKASVNFVGKTSMEVGVRVEAENPISGV
                     KRHTGSAYLTFVALDANKKPMQLPPLILETEEERLRNAEASERRQMRLAEKKKEKKKH
                     EK"
BASE COUNT         7155 a         7878 c         8995 g         6302 t
ORIGIN      
        1 acggagcagg gcagcgtgga ggtaaaagta aatacggaaa aagcgtcctt cggcggcgaa
       61 aaagacgatc tttgccttgt gcacatttcc gtaaaggaca cgggcatcgg cattccgccg
      121 gaaaagcagg gcatgatttt tgagcgcttt acccaggccg actcctccgt aacgagaaag
      181 tacggaggaa ccggtctggg cctgtccatt tcccgccgtc tggcggagct gatgggaggg
      241 cgcctctggg tggaaagcac gcctggtcag ggaagcacgt ttcattttac gcttttgctg
      301 cgcattgaag acaaaacacg gcaggcggcg ccgcctgcaa aaagcgaaaa ggcggctgtt
      361 cccgaacagc gcccgttgaa aattctgctg gtggatgatt cggacgacaa ccgtcttctg
      421 gtcaagacgt atctcaaggg cgatgcctat caaatcgatg aggcggaaaa cggcgagcaa
      481 gcggtagcgc ttttccagtc cggtctttat gatctggtgc tcatggacat gcagatgccg
      541 gtgatggacg gttatacggc cacgcgcgcc attcgccggt gggaagagga gcagggcaaa
      601 aatcggacgc cggtgatcgc gctgaccgcc tacgccctca cggaagacgc ggccaaaagc
      661 gaagaggccg gctgcgacac gcatctgacc aaacccgtca aaaaagcggt tctcctcgag
      721 gccatccgcc ggtttgccgc tccctgagcc ccatccgccg ttgacagttg agcttgggga
      781 gaaaagggga tttacacggc gttgcaccct tcaaacagca attatcaaca gtcagaatct
      841 aatagttcat cttgcagtga ggcccggcaa gtgataaaag cctccagcca ttataggaaa
      901 ggtttgcata gatattcatg aaaaagaaac tgatttccct gatcgcgcgt tccgtcgggg
      961 cctgcgccgg caagggactg ctgccggacg tggatctttc gcgcatcgaa gtcgaagtgc
     1021 cggccaatcc ggagcacggg gactacgcct ccaatatcgc gatggttctg gcctcccagg
     1081 ccagacaaaa cccgcgcaaa atcgcccagg cgattcagga aaacctcgat gatccggaag
     1141 gcatcgttga aaaaacgcaa atcgccggcc ccggcttttt gaatttcatg ctcaaagacg
     1201 atgtatggcg aagcgttttg aaagatattc tggatcagaa ggaccggtac ggttgccttc
     1261 cggcaggctc cgggaaaaag gtccaggtgg aattcgtcag cgccaatccg acggggccgc
     1321 tgcacatcgg gcacgcgcgg ggagcggtgg tgggcgatgt gctctgcaat ctgctggcgg
     1381 cgaccggcca acaggtttcc cgggaatatt acatcaacga cgcggggaac cagatgaaca
     1441 acctgggccg gtccgtgctg tatcgtctgc gggagcttgc gggagaagcc gtcgaatttc
     1501 cggaaaacgg ctaccgcgga gactacatca gggaaatcgc cggcgatatt ttcaaaaagg
     1561 aaggaagccg tttccgggat gaaaacgagg aggaaacgat tcagcgcttc aacgaagcgg
     1621 cgggcgcggt gattctcgac ggaattcgga aggatttgcg ggatttcggc gtgacctttg
     1681 actgctattt cagcgagagg gagctgtacc gggaaaacgg cgtcggacgc ctgctggacg
     1741 aactgcaaaa gcagggcttc atttattccg acggggagac gctgtggttt aaaacgaccg
     1801 attacggcga cgacaaggac cgggtggtca tccggaagaa cggagagccg acctacttcg
     1861 cggccgacat cgcttatcac gccaacaagt tttcccgcgg gttcgatctg ctcatcgaca
     1921 tctggggcgc cgaccaccac ggctacattc cccgccttga ggccgcgatt caggccatcg
     1981 gacggcccag ggaagatctg aaaatcattc tcgtgcagct ggtcaacctg ctgcgcgcgg
     2041 gcgagccggt ggccatgtcc acgcgctccg gcgaattcgt cacgatgcgc gaagtgctgg
     2101 acgaagtggg cagagatgcg gcccgctata atttcctgat gcgccgctcc gacagccatc
     2161 tggatttcga cctggaagtc gccaagaaac agtccaacga aaacccggtt tactatgtcc
     2221 agtacgccca cgcccgaatc tgcagcattc tccggacggc ccaggagcgg ggaatgaccc
     2281 tgcctgattt tccggcgatc cgtccatccc tgcttgccga agcggaggaa agaacgctga
     2341 tcaaaatgat ggcgcgctat cccgaaatgc tggaaggggc ggcaaagacg ctggaggtcc
     2401 accgcatcac cttttatctg aatgagctgg ctgccgtatt tcacggctac tacaacaagt
     2461 acaaggtagt cacccaagac gcggcgctat cgaacgccag gctggtgctg gtcgcggcgg
     2521 tgcgcgaggt gctgaaaaac gcgcttggaa ttctgggcgt gggcgcgccg gaaaaaatgt
     2581 aggagggagg ccatgggctc ggggaatacc aggaaatttg aaatcaggct ggggcagacg
     2641 ggattgacgg tcctgatcgt cggcatggtc gtcctgctgt gcctttcctt tcttttcggc
     2701 gtcggcgtag gaaaaaacat cgacacctat cccgaaaaga ttgcctccct gccgcagcgg
     2761 tttctggcgc tcttctggaa gccggccaag gtcgccgtag agcagaaatc ggttcccgtt
     2821 gcgcaggcgg gcaaggccaa tgtggacctg gcgtttcatc aggcgctgac cagttccaaa
     2881 accgtcccga ttcaacccct gccggatgcc gaaaagacgg ccgaggttga agccttcgcg
     2941 gacagggaag aacctttgcc gccgccttca tcgccatcgg ctcaaaagga aaaggccgcc
     3001 ccgttgaaac ctgaaccagt cagcccgaag aaggccgccg gcgaaaagcc cgccgcggaa
     3061 atcaaatcaa aagaaagcaa gaccgattcc gccgccgcat cctatcaggt gttcgtggcg
     3121 tcgctcaaag acaggcaaaa ggccgaccag attcacagga tcgtcgcccg ggcgggatac
     3181 gcgtcgaaaa tcgtaaaggt ggatctgaaa gggaagggca cgtggtatcg cgtgatcgcc
     3241 acgggattcg acacccgcgc caaagccgag gcagccgccg acaagctttc caagagggcc
     3301 aaaacgaaat gcatcgtgcg cccaaccgcg gccggaaagg cggcgaatcc ctgaggaaac
     3361 aaagccggcc ccgcacgggc gaggcgcgat gagggaaacg gaaatattgt gaaggtttga
     3421 aatatgtttg aaagtctttc ggaaaaactg gaaggtattt ttaaaaagct caagggaaga
     3481 ggcgttctca acgaggaaag cgtcaatgcc gcgcttcggg aaatccggat ggcgcttttg
     3541 gaagcggacg taaacttcaa ggtcgtcaag gattttatcg aagacgttcg ggggcgcgcc
     3601 gtcggcaaag aagtgctgga aagcatcacg cccggacagc aggtggtcaa gatcgtgcac
     3661 gaccgcctgg tggagctgat gggctccacc agcgccgcca tcaagttcgg tccgaggatc
     3721 cccgcgccga ttatgctggt cggccttcag ggctgcggaa aaacgaccac ggccgctaaa
     3781 ctggcgcggc tcatttccgc ggataaaaga gtttacctgg tgtccgccga cgtttaccgg
     3841 cccgccgcga tggagcagct tgccgttttg ggccggcagg tcaaggccgg cgtttttgaa
     3901 gccggctctt gcaaagatcc cgtaaaaatc tgcgtggacg cggtcgaaca ggcccgaagg
     3961 gaaggctatg aagtcatcct gatcgacacc gccggacggc tgcacatcga cgaggagctg
     4021 atggacgaac tccgccggat cagggaaaag gtcaacccgg ccgaaattct gtatgtggcc
     4081 gacgcgatga ccggccagga cgcggtcaac gtcggggcca agttcaacga gctcatcggc
     4141 attgacggcg tgatcatgac caagatggac ggcgacgcgc gcggcggcgc tgccctgtcg
     4201 ctgcgatccg ttgtgggcaa gccgatcaag tttgtcggcg tcggcgaaaa gatcgacgcg
     4261 ctggaggttt ttcatccgga gcggatggcc ggccggattc tcggcatggg ggacattctg
     4321 tcgctggtgg aaaaagcgca ggccaccatc gatgaaaaaa ccgcgaagca gatgcaggaa
     4381 agcctgcgca agagcgattt tacgctggag gatttccgca aacaactggg gcagatcaaa
     4441 aaaatggggc ccctgaagga catgatcgga atgatcccgg gcatgggcaa aatcaaggcg
     4501 ctgaaagaca tggagccgga cgacggggag ctggtgaaaa tcgcggcgat gattgattcc
     4561 atgacgaaga aagaacgcaa caattacctg atcatagacg gccgcaggcg caagcggatc
     4621 gccctgggaa gcggaacgac ggttcaggac gtaaaccgca tgctgaaaaa ctacatagag
     4681 cttcggaaaa tgatgaaaaa aataaattcc aaaggcggca tgaaagcgtt gatgaggaat
     4741 ttcccctttt aaaatcaaaa tatttgtgat aaaaaatctg acactttttt ttgaaggagg
     4801 tttggagtaa aactacatgg ccgttaaaat cagattgacc cgcatgggtg cgaaaggcaa
     4861 accgttttac cgtatcgtcg ccgcggataa agaatatccc cgcgatggaa gattccttga
     4921 aatattagga aattatgatc ccaagaaaaa cccggcggaa atcaaactca aagaggaccg
     4981 cgttcgcgac tggctgtcca aaggcgcaaa gccgacgctg accgtttctc atttattgaa
     5041 aacaaaaggc atcgttgccg ccgtcaagta gacgggccgc ggccgatcac gcttgcaaaa
     5101 tacaggatta gaacctcaca ccatggaatg aaacaagctg tgtgaatgtt ggaatgattt
     5161 ttcagacgca ttaaccaaaa ccagtggagg ggctgacgat gaaagacctg attaagtaca
     5221 tttctcaagc tttggttgat aatcctgatc aagtggaagt cacggaagtc atcggagagc
     5281 agacctccgt catcgaactg cgcgtggcca aagaggacct gggcaaagtc atcggcaagc
     5341 agggaaggac ggccaaagcc atccgcacca ttttgagcgc cacttccgcc aaagtccaca
     5401 agcgggcggt gctggaaatc atcgagtaaa atggatttgt ttgttttcgg ggagatcgtc
     5461 aaaacccgcg gtctccatgg ctgtttgaaa gccctgtctt ttctggagtc atcggagctt
     5521 ctcgacggac tgacggatgt gtacctggaa accccgtccg gccggaaaag ccgtcatgaa
     5581 ataaaaaaaa taaattcctc cggtaaattc ctgtttctgg aaatcaagga tgtgtccggc
     5641 gtggacgagg cccgaaggct tgtcggatcg aggctgctca tgccgcgcga caggcttgcg
     5701 gctcttgcgg ccgacgagta ttactggcac gacatcgtcg gacttgacgt ttacacgccc
     5761 cggggccgcc atctgggaag gatcgagtcc gtctttccca ccggcggcaa tgatgtctat
     5821 gtctgccggg gggacgcggg tgaaatcctg attccggcga cggccgaagt ggtcctgcag
     5881 gtggaccttg ccggaaaaaa aatgatcatc caaccgctgg aaggccttct gccgtgatcc
     5941 gatttgatgt tttgtccatt tttccggaaa tgctgcaatc gcccctggaa ttcagccttt
     6001 tgaaaaaggc gcgggacaag ggacttgtcg aagtcggcct gcacaacatt cgggactggg
     6061 cggaagacaa acaccgcatg accgacgacg ccccctacgg cggcggctgc ggaatggtga
     6121 tgaaagtcga gccggtggcg cgggcgctgg cggcggtgaa gcgcccggtg gggccgtcgc
     6181 ttacggttct gatgacgccc cagggggaaa tctttcagca gaagatcgcg gctgagctgg
     6241 cgagccagga acagatcatc ctgatttgcg gacggtatga aggatttgac gaacggattc
     6301 gggagcatct ggcggaccgg gagatttccc tgggcgacta cattctgacg ggaggggaac
     6361 tttccgcgct cgtggttatt gatgccgtct ccaggctgat tcccggcgtg ctgggaaacg
     6421 aagactccgc gtcgagggaa tctttttcgc aggggctttt ggaatatccg cagtacacgc
     6481 gccccgccag tttttcaggg tggagcgttc cggaagtgct ggtttccgga aaccatgccc
     6541 agatcgagcg ctggcaacgc atcgaggcgc ttcgccgcac ctggcaaaga aggcccgaac
     6601 tgctcgaaaa agccgggctc ggcggggaag atgaggaact tttggaaaaa attaagctgg
     6661 gccggatata aagtcttgat tttcagacgg gtttggggta atgaaaccag cctttagggc
     6721 acagcgattc gtatttgaaa agcagcagga ggagtgagga catgaatgta ttggaaatga
     6781 ttgaaaaaga acagatgaga ggcgatattc ccgccttcaa atcgggcgac accgtcaagg
     6841 tatatgtgcg gatcactgaa ggacagaagc agagaatcca ggcttttgaa ggcgtggtca
     6901 tccgcaagcg ccgcggcaac agccgggcaa atttcacggt tcggaaggtg tcctacggca
     6961 tcggcgtaga aagaaccttt cccattcatt cgccggtcat tgatcgcatc gaagtggtgt
     7021 cccgcggttt ggttcgccgt tcgcgcctgt attacctgag aagccttcga gggaaaaagg
     7081 caaggatcaa ggaagacacc ggccactgag gccgttcagg gtttttcgat gaatacattc
     7141 gaaagacttg cgcaagtgga aggctacaaa accatcgcgg gtgttgacga agcgggccgc
     7201 ggcccgctgg ccggccccgt ggtggccgca gccgtaattt ttccgcccga atattcaaat
     7261 cccgccatca acgattccaa gaaactgacc gccgccaaac gcgaagatct ctaccgggtc
     7321 atcacccggg aggcgatggc cgtaggcctc ggcgtggtgg aagcggacgt catcgaccgg
     7381 atcaacattc ttcaggcgtc gcttcaggcg atgcgcgaag cggtgacgga gctttccgtg
     7441 aggccggatt acctcctcat cgacgggctc aactccatcg acttcgacgc gccgcagaag
     7501 gcgatcgtca aaggggattc actcagcgtt tccatcgccg ccgcgtccat cattgccaag
     7561 gtttcacgcg accggatcat ggagatgtat caccgccagt ttccccgcta caactttctg
     7621 aaaaacaaag gatacggcac ggtcgggcat cgcagggtcc ttgaggaaat cggcatgtgc
     7681 aagattcatc gccggtcctt tcatctcaaa aaacagaatc tcccgcaaac ggcgctggac
     7741 tggtcagaat gaaggattca caagacaggc gaaaaatcat caccggcagg gccggggaag
     7801 cgatggccgc cgattttctg aagcggcggg gctaccggat acttgagatg aactaccgct
     7861 gttccatcgg ggagatcgac atggtcgccc gcgagaagga cgtgctggtg tttgtggagg
     7921 tcaagacgcg aaaatccggc gcgatgggct atcccgagca ggcggtgggt ttggtcaaac
     7981 aaaagaaaat gtctcagctc gcgctgtggt atctgcggga taaaaatatc ggggacgcag
     8041 cggtccgctt tgacgtggtg gccattttgc tgcttccctc cggaaacgac attcgactga
     8101 tccgcggcgc tttcgacttc attgccctgg aatagcccgc gttgtgcctg cggctttgcc
     8161 ggcaggcgcc ggacgcgaga agctaaaagc gggtgagggc cggctttccg atgggaaaca
     8221 cgttgaagcc gatctcaaag cattttcggt gatcgacgat gttgcggccg tcgaacagga
     8281 aggcgggttt gaccatcgat ttgtagatct tccggtaatc cagcttccgg tattcctccc
     8341 agtccgtcat gacggccagc gcgtggcagc cccgcgcggc ctcgtagggg tcttcgacat
     8401 aacggatatt tcccttttca tgcgccagat cgatcgcggc attggccagc gcccggggat
     8461 cggtaatgat cacttcagcc cgctccgcca ggagctttcg tgtgatggcg atggccgggc
     8521 tctcccgcgt gtcgccggtg tcggctttga aggcaaatcc cagaacgcag attttctttc
     8581 cggccagcgt gttgaacatg gaccgcagaa tgttggcgat aaaccggtcc tgctgatact
     8641 cgttgatcct gacgacgctt tcccagtacg aggccacttc cggcagaccg taaaaacggc
     8701 acaggtacac gaggttcaga atatctttct tgaagcagga gccgccgaag ccgatgcccg
     8761 ccttcagaaa tttggcgccg atgcggctgt ccgtgcccac ggcgtgcgcg acttcggaga
     8821 tgtcggcgtc cgccttttca caaagcgccg atatggaatt gatggaggat acccgctgcg
     8881 ccagaaaggc gttggccacc agtttggaaa gttcggcgct ccagatgttt gaggtgatga
     8941 tcttttctcg cggcagccag cgggtgtaaa tttccacaag cgcggcgcgg gcggccaggc
     9001 cgcttctggt ttcgcgcgaa ccgatcaaca cccggtcggg attttccaga tcgctgacgg
     9061 cggttccctc cgccatgaat tccggattgg aaaggacgtc aaacgtcacg tttttctttc
     9121 ccgacgacag aatccgttcc atggcctgcg ccgttttgat cggcagcgtg cttttttcaa
     9181 tgacgatttt cggggttttt gaattttcga gaatctgccg ggcggttttt tcccagtact
     9241 gcaggtcggc ggccatgccg gcgcccgcgc cgaaggtttt ggtcggcgtg ttgacgcaga
     9301 cgaaaatgat gtcgttttcc tgaatctgcc cttcgacgtc cgtggaaaag aaaagattcc
     9361 ggccgcgcgt tttccggacg atggcgtcca ggccgggttc ataaacggga agccggtcgg
     9421 agttccaggc ggcgatgcgc tcgggattga tgtccacgac ggtcactttg tagtccgggc
     9481 atttggaggc gatcatcacc atcgaggggg ctccgacata gcctgcgcca atgccgagaa
     9541 tttttttctt gaatttcatg ctgttccgtt tcctcccgcc ttggtcaggc tgttttcaga
     9601 atttctttga aataggcaat cgtgccgatc aggccctttt ccagcgaaat cgtcggctgc
     9661 catcccagtt tttcccgcgc cagggcaatg tcgggtttgc gctgcgccgg gtcatcggac
     9721 ggcaagggct gaaagacgat ctgcgatgtg ctgcccgtca gttctatcac caggcgcgcc
     9781 agctgcagga tggtgaattc gccgggattg ccgatattca ccggtccgaa gaaatcgtcc
     9841 ggcgtgttca tcagccggat cagtccgtca atcagatcgc ctgcgtagca gaaggagcgc
     9901 gtgtggctgc cgtcgccgta aacggtgatg tcccggttct gcagggcctg aaggatgaag
     9961 ttgctgacga ctcggccgtc gtttggcgcc atgcgcggtc cgtaggtgtt gaagatgcgg
    10021 gcgatggtcg tgcggacggc gttttgccgg cgatagtcca tcatcaggca ttccgcggct
    10081 cttttgccct cgtcgtagca gcttcgaatg ccgatgggat tgacgcggcc ccagtaggat
    10141 tcatgctgcg ggtgtacttc cggatcgcca tagacttccg aggtggaggc ctgaagaatt
    10201 ttcgcccgcg ttcgtttggc gatgcccaga acgttgatgg cgcccatgac gttcgttttg
    10261 atggttttga tcgggttgaa ctggtagtga acgggcgacg ccggacaggc gaggttgtag
    10321 atttcgtcca cctcaagata gatggggttg atgatgtcgt gccggatcag ttcgaaatgc
    10381 gtctgtccca gcaggtgcga gatgttgttt ttactgccgg taaaaaagtt gtccaggcag
    10441 atcacatcat gtccttcgcc gcaaagccgc tcgcacagat gggagcccaa aaaacccgct
    10501 ccgcccgtaa tcaggattcg cttcatatcc ggttggctgt tcttccctgt agtaatttat
    10561 tttcgtctct gctaataaag ccgaagggct cggatgtcaa ccataaaaag tcagaggggc
    10621 ggcaaaatgg acagggaagc gcggggagtt ctttacatgg tggccacgcc gatcggcaat
    10681 ctggaggata ttacccttcg ggcgctgcgt gtgctgaaag aggttgatct cgttgccgcg
    10741 gaggacaccc gccgctccaa aatcctgctt tccgcgcatg ggatcaaaac gccgctccgc
    10801 agtctgcatg agcacaacga gaaggagcga agcgccgaaa ttctatccag gctccaaaac
    10861 ggaaagagcg tcgcctacgt ctcggatgcc ggaactccct gcgtctccga tcccggccgg
    10921 catctcgtgc gggcggcccg cgacaacggc attcgcgtgg ttcccgttcc gggcgcctgc
    10981 gctttgcccg cggccctgtc ggtttgcggc tttcaggccg aaccattttt gttctgcggc
    11041 tttctccccc cccggtccaa ccgccgccgc cgctttctgg agtccattat ggaagaggaa
    11101 tgctccatca ttttctatga gtctccggcc agaatccgtg aaacgctgga agacgcgctg
    11161 gacattctgg gcgaccggca ggtcatggcg gcgcgggaac tgaccaaggt gtttgaagag
    11221 atccgggagg gcaagatttc cgaactgctg gccgcatggc ccggcgcggg cgtcaagggg
    11281 gaatttacgg tgatgcttca gggcgccgcc cgcgagcctg cggttctttc ggagcgggag
    11341 atcggggaac agcttttgaa aatcctttcg gagaaaggcg tttcgctgcg ggatgccgtt
    11401 gcggaagcgg cgcgccgaac ccaaatgccc agaaaaaaaa tatacgatgc ggcgcttcga
    11461 atccgccggg aatttccgta agggagcggg gccgccgcgg tttacatatt gcttgaagag
    11521 ttgccgtcac tatgctaatc ccatgtcgaa atgaaagcaa ttccccgcgg tcgggggaga
    11581 gccttccgct tcgggggaga agtttttttg cgacccccgg cggcaaacaa attcgccgcc
    11641 cgattgaaaa aagggagttg tcatgaaaat ccgtccggtt tttttcgctc tgatcattgc
    11701 gctggccctg tgcctttgcg cctgttcgtc gcgcggcccg gatacgctga aaaatgatgc
    11761 cggcacaaaa gcggtgaacc ggattgccct gctgccggtg gagaatcaaa ccggagacgc
    11821 cagggtgccg aaaatgctcc gtctcaaatt gtcggaagaa cttcgcttta aaggctatcc
    11881 ccaggtggat atcgaggtca ttgacggccg tctggccgcc atggcgggcg ccggcgaacc
    11941 ggggaagacc gcgattgctc caggggttgt ccgggaaatg ctgggcgcgg acgccgccat
    12001 gtactgcagc ctgatggaaa gcaaagtttc caaaaagatt ttttacacgc cggtcaccgt
    12061 gtcggtgcgt tgcgaattgc gcagcacggc aaccggcgag accctgtgga acgcagaggg
    12121 caaggcgacc ggcagaagct ttgatctcag aagcaaaacg ctggacctga aagccctggg
    12181 agatctggaa gcggttctgg aaaaggtggt ggacgaggtg atggaatccc tgccgtacgg
    12241 accgaaattg agcggatagt ccgccgcggt ccgtccggaa atgaggggtc aaaccggtct
    12301 cggaaagatg caaaggagaa acgcatgagg gaaaaaacga aaatcatcgt ggctgtgatt
    12361 atgacgctga tgctcgccgc cgccgcgcag gcccagtcgc cggtgaccgt caaaatgacc
    12421 aaaggacggg ctcagatcac atcgttgaag ggaaaagttt ccatggtctg tccgggcggg
    12481 aaggaggcgt tttacctcaa aacctatgat ttcatcctgc ccgggtgcga agccgccacg
    12541 ggcccggact ccagggtgga gatggttttg cccgaccgaa gcgttgtccg cttctcggag
    12601 aagacaaaat tcaaactggt ccaggccgac atcggcgcac ggggaagccg ttcggtcggc
    12661 atttccgtta cgctcgggaa aatctggaca aacgtgcgca agtccctggt gggcggaagc
    12721 gaccggtttg aaatctcctg ccagaatgcc gtagccgggg ttcgcggcac tgtttaccgc
    12781 atggatgtcg aaaaggacca gtccgcggtg gtcaaggttt acgacggcga agtcagcgtc
    12841 gcgggtgtct ccccggcgca gcctaaagcg ccggtcgcct tcgggcctcc caaaccggtt
    12901 gcagggccga cggtgattga agggccgaag cccgtctcga tggagcagtg ggtgtatatc
    12961 gtccggtcca tgcagcagat tcaaatcacg gtcgacggcc aggcccagaa gccgagggat
    13021 tttacgcaag aagaggacat ggatgactgg gtgaagtgga accaagagag agataagcag
    13081 tacggccaat aacgtcgggc gcagaatgta tagatgaata tccctaactt tctatcgctt
    13141 ttgcggatca tactggttcc ggtgttcgtc atctttctga ttcaggcgga atatgaccgg
    13201 gcgctgatca ctttcgtcgt tgccggcttg acggacgcgc tggacggaac gctggcccgg
    13261 cttttgaaat gccagaccgt tctgggcgcc tatcttgacc ctcttgccga taaacttctt
    13321 ttggccacaa gctttatcat gctggccgtg atcggtctca ttccggcatg gctggccgtg
    13381 gtggtcatca gccgggactt gatcatcctg ctgggaattg caatattctc actgatgtcg
    13441 attgcttacg aaatcaagcc ggcgattatc agcaaggtga cgacggccct gcaggtgggc
    13501 accatttttt ttgccctgct gaacaaggct ctgccccagg attttggaac attttggatc
    13561 gccgtgcttt tctgggccac ggcgttgttc acctttggtt ctggcgtcgt ttacattctg
    13621 cgcgggatac ggatactgaa taaatccgca cccgaagaga cgaaaaagta agatattatc
    13681 ttgacacgaa agcgttttgc tgttaggtga ctcaccgcaa aagttgaaga taggcaagta
    13741 gctcaggggg agagcgccat cctgacgcgg tggatgtcca gggttcaaat ccctgcttgc
    13801 ctaccatttt ttatcttcgt gcatatgccg ttcgtgccga taacagaaaa gggcatcagc
    13861 aaaatggtga tgccctttta aaatgattga aaattttgag atccgctgat tgaaatgatg
    13921 gatataaaag taatttttcc ggaccaatcc cagcagacgc aaccggcggg tgtgtccgtg
    13981 gccgaggcca tcggaagctg gaagaaagaa gcgctcgcca ccgccgtcgc cgcaagcgta
    14041 aacggcgctt ccgtcgatct gaactttgcg ctggcggaag acgcaaaagt tgagcccatt
    14101 gatattgcct ccaaaaaagg tctttccgta ttgcgccaca gtatttctca tgtgatggcg
    14161 caggccgtgc aggatgtgtt tcccggcgca aaagtaacga ttggtccttc cattgaggac
    14221 ggattttact acgattttga atatgccgaa accttcaccg tggatgattt tgaaaaaatc
    14281 gaaaaaagga tgaaggaaat cgtcgcggcc gattatcctt ttgtccgcga ggagctgccc
    14341 cgcgaaaagg cggtggatct gtttgccggg aagggcgaag attacaaggt ggagctgatt
    14401 cgcgatctgc ccgcggatgt ttctgtggtc agcctttaca aagacgggga ttatgttgat
    14461 ttatgccgcg gcccgcacat tccgcggacc ggcatgatca aagccttcaa gctgctgagc
    14521 gtcgcgggcg cctactggcg cggcgacgag cgaaacaaga tgcttcagcg catttacggc
    14581 acgggctttg ccgacgagca ggcgcttcag gattatctga cgctgctgga agaggccaaa
    14641 aagagagacc accggaaact gggccgcgag ctggatctgt ttcaggtcaa cgacgaagcc
    14701 ggggcggggc tggtgatttt ccatcccaag ggcatgctgc ttcgctatct tattgaggaa
    14761 tgggaaagaa aagagcattt gaagcgcggt tacgacatgg tgatgggccc ccagattctc
    14821 aaggtggatc tgtggaagaa atcgggccat ttcgatcact accgggacaa tatgtatttc
    14881 actgaagtgg atgagcagac ttacggcatc aagccgatga actgcctgtc gcacatgctg
    14941 atctacaaat caaaaatccg cagctaccgc gatctgccgc ttcggtattt tgaactcggc
    15001 accgttcatc gccacgaaaa gaccggcgtt ctccacggat tgatgcgcgt acgccagttt
    15061 acgcaggatg acgcgcacat cctgtgcatg cccgaacagc tgaacgcgga aatccgcgcc
    15121 atcgccgact ttgtccatta cgcgatgggc attttcggct ttgaatatga agtggaatta
    15181 agcacgcggc cggagcactc catcggatcc gacgcggact gggatctggc cacaaaggcg
    15241 ctggaaaacg cgcttgtgga caacaaaatt ccctacgaag tgaacgaggg cgacggcgcg
    15301 ttttacgggc cgaaaatcga cttcaagctc aaggacgccc tgaagagaaa atggcagtgc
    15361 gcgacgatcc agtgcgattt caccctgccg gagaggttcg atctgagcta catcggcccc
    15421 gacggggaaa agcaccgccc cgtgatgctg cacagggtca tcctgggggc gatcgaacgg
    15481 ttcatgggcg ttctcatcga acattacgcc ggagcgtttc cggtatggct ggcgcccacc
    15541 cagtgcgttg tgctgacggt tacggacaag cagattccct atgccgaatc ggtgcgcggc
    15601 cgattggtcg aagccggcat ccgttgtgag ccgtatttcg acaacgacaa gctggggtac
    15661 aaaatccgcc aggcgcagat gcagaaggtt ccctacatgc tggtcatcgg cgacaaggaa
    15721 gtggcggcct gtgccgtggc gccgagagcg cgggacggcc ggaatctggg cgcaatgccc
    15781 gtggatgaat tcattgcgct gattcaagaa gcgtgcggga gcaaaaagta gcaatcgact
    15841 ttgggaagga caatccggga ggtgccgtat agctaaggat ttaaggatca accgagaaat
    15901 caagtcggcg aacgtcagag tcatcaacga agagggccag ccgctgggcg tgatcccgct
    15961 ggaggaagcg atggcacaag cggaaagagc ggccctggat ttggtggaag tgtcggccaa
    16021 cgccgatccg cccgtctgca aaatcatgga ctacggaaag taccgctaca agcagagcaa
    16081 aaaaatccat gatgcccgga aaagccagac ggtgattcat gtcaaggaaa tccgattgcg
    16141 gcccaaaacg gaagcgcatg atctgcaggt caagatcaag cacatcaaaa agtttttgga
    16201 gcagcgcgac aaggtaaaaa tatccatgat cttccggggg cgcgaaatcg cctttaccga
    16261 gatcggccgc aaaatcatgg agcagatcaa ggcggcgctg gccgatgaat gcatcatgga
    16321 ccaggagccc aggctggaag gccgcagcat ggtgatgatt gtttcaccga aaaaataaat
    16381 ccgaggggat tcaaaaagag gtgagaagat gccgaaaatg aaaacacatc gaggggcggc
    16441 caaacgtttt tccgtgaccg caaaggggaa ggtcaagcgc agcaaggcgt ttgccagcca
    16501 catcctcacg aagaaaacca ccaaaagaaa aagaacgctg aggaaagccg cgctggtgca
    16561 tagcgccaat gaaaaagcca tcaaaaggct gattccttat ttgtaggccg taaatctgcg
    16621 gaagttgttt tatatttgag gagagtaaaa gaaaatggct aggatcaaaa gaggcgtcac
    16681 cgccaggaag aaaagaagaa aaattttaaa aatggccaag gggtttttcg gagcccgcag
    16741 ccggctgctg cgaaccgcga cggaagccgt caacaaggcc atgagctatg cctatcgcga
    16801 ccgcaggggc cgcaaacggg agttccgcgc gttgtggatt gcccggatta acgccgccgc
    16861 ccgtcaaaat caaatttcct acagccgcct catggacggt ttgaagaagg cgaacatcgg
    16921 tctggatcgc aagattctgg cggagctggc cgtcaatgat ccgcagaatt tcgctcggat
    16981 tgtccagatg gccaaaggcg agcaggcggc atgattgcgg atcttcagca tctggaaaag
    17041 aatgctctgg ccgaactgga aaagaccggc tccgaggatt cgattctggc tgtgagaaca
    17101 aaatacctcg gccgcaaagg ccttcttacc ggtttgctga gaaatatctc ttccgtgccg
    17161 gccgaagaaa ggcgttcttt cggaaagcgc tgcaacgaag tcaaggagct tctggaaaaa
    17221 aacatcgaag aagccctgcg gcgtcggacg gaggatcaaa aggccgggat gctggccggc
    17281 cagcagattg acgttaccct gcccggcaga ggcatccgcg cgggcagcct ccatcccgtg
    17341 gttcaaatcc gccgggaaat ttgcgatatc ttcgcggctt ttggattttc cgtggtcgag
    17401 gggccggaag tcgagctgga ctactacaac ttcgaagcgc tcaacattcc caaagatcat
    17461 cccgcccggg acatgcagga caccttctac attgaagaaa atatggtgct gcgcacgcat
    17521 acctcgcccg ttcaggtccg gattatggag aaggtaaaac cgcccgtgcg cattttgtcg
    17581 ccggggcggg tgtaccgtcg ggattccgat gtttcccaca cgccgatgtt tcatcaggtg
    17641 gaggggttgc tggtggatcg cggcgtcagt tttggcgatc tgaaaggcat tctgaccgct
    17701 ttcttgagga aagttttcgg ggaagacacg attctgcgct ttcgacccag tttctttccc
    17761 ttcacggaac cgtccgcgga ggtcgatatc cgctgcgtca tgtgcggcgg caaaggatgc
    17821 cgcgtctgcg gacagtccgg gtggctggag attctgggct ccggcatggt cgaccccgcc
    17881 gtctttcaga acgtcggcta cgacccggaa gtttataccg gttttgcttt cggtatgggg
    17941 ctggagcgca tcgccatgct taaatacggc atttccgaca tccgtcttct ttttgaaaac
    18001 gacattcgat tcctgaaaca gttttaggag aatgtgattg catgctggtc agtctcaaat
    18061 ggcttaagga ttatatcgac gtggaaacgg ctcccgagga gcttgcggac cggctgacca
    18121 tggccggtct ggaggtggat gagattcgca cgctctcccc cagattttcc ggcgtcgtgg
    18181 ttgcgaaaat tttatccgtc cggccgcacc ccaacgcgga caagctttcc ctgtgcgatg
    18241 tcaccgacgg cgcgcagacc tatcccatcg tctgcggcgc caaaaacatc catccgggcg
    18301 acatcgttgc gctggcaaaa atcggcgcaa tcatacccgg cggctacacg atcaaatcca
    18361 ccacgctgcg cggagaaaaa tccgaaggca tgctctgctc cgaggcggag ctggaaatcg
    18421 gcgacgacgc ttcgggaatt gtgcagttgc cggccggcat gacgccggga acgccgctgg
    18481 aagaggccct ggacctgaac gacacggttc tggacgtcgg cgtcaccccc aaccgttcgg
    18541 actgcctgag catgatcggc atggcccgcg aagcggccgc cctcaccgga aagaccctga
    18601 aaatgcccga agggaaggtc agggaagcgg ccgaagacgt gtcgctgctc tcgtcggtaa
    18661 ccattgaaga tccggacctg tgcccgcggt acacggcgcg gctgattcgg aacgtgacca
    18721 tcggcgattc tcccgtctgg atgaaaacga ggctcgaggc cgccggcttg cgcgccatca
    18781 acaacgtggt ggacgtgacc aacttcgtca tgctggaaat ggggcagccg ctccatgcgt
    18841 tcgattttca cttcctggaa gagaggcgca tcgtggtgcg caaatcaaag cccggcgagg
    18901 aattcgtttc actcgacggg aaaagccgca tccttcccga aaatacgctg ctgatttgcg
    18961 acggaaaaaa gcctgtggcg attgccggga tcatgggggg actgaattcc gaggtcaaag
    19021 aagacacccg gacgatattt ctggaaagcg cctatttcaa tccctcatcc attcgccgtt
    19081 cgtcacgccg tctggccatg ccgacggacg ccgctttccg ctttgaacgg ggcattgatc
    19141 ccgaaggcgt tatccgggcc ctggacaggg cggcacagct gatcgccgat ctttccggcg
    19201 gcgaggtctg cgcgaaccgg ctggacgaat acccggcgaa aatcaagccg gttgaaaaca
    19261 tacttctgcg aatggaccgc gccgggcagg tgatcggagt tgccgttccg gccagggagg
    19321 ctgttcgcat tctgaagagc atcggcatga cgctgaagcg cgagggcagg ggagtctatc
    19381 gcgtgacgcc gccctcgttt cgggtggata tcgaaagaga aatcgacctg atcgaggaaa
    19441 tcgcgcgtct ttacggttac gaccgggtgc cttcgacgct gccgtccgtc tcagtgacgg
    19501 agctggacgc cattccccgc ctggcgcttg aagagagaat tcgcctcctg ctcacgggct
    19561 ttggctattc ggaaatcatc aactacagtt tcacgacgcc cttttgcgcg gaagcgctcg
    19621 gcctgccgcc ggaagatgaa aggcgacggt tcgttgtcat ccggaacccg ctgacggaag
    19681 accagtcggt catgcgcacg acgctgacat acggtctgct ggaaaccctg aagaagaaca
    19741 tcaacaatgc ctcttggaat ctgaagctct ttgaaatagg acggactttc ctcaggcgcg
    19801 gcgatggcga gctgccggaa gaaagaaata tcctggcggc gctcgccgcg ggcaaggcat
    19861 cggatgacct ctggggattg aaagccggtg ttgactttta tgatctgaaa ggctgcctgg
    19921 aaaatatttt tcaggatttg aagttcgact ccatgcggtt ccgggcggaa aagccccagt
    19981 cgtatctgca ccccggtcag tcctgcggcg tgtatgcgga tgatgtccgg atcggctcca
    20041 tgggaaaagc gcacccggat gttctgaaaa aaatggacat caagagtgac gcttatgtat
    20101 ttgaaattga tcttgatctg ctgggcgcaa agatacggcc gcgggttcgc tgcagggaaa
    20161 tatccaagtt tcctgcggtt ttgcgcgatg ttgcttttgt tcttccctcg tccatggaat
    20221 ccgaaaaaat gcttgaaatg gttttgcgtc agcgtgaaga tttgcttgaa aatgtttcaa
    20281 tttttgatat atacgcgggc aaaggtcttg aagaaggaac caagagcctg gggctcaggt
    20341 tttcctaccg cgctttggac agaacgttga cggatgcgga agtcagtgcc attcacgacc
    20401 gaattgtgaa ggacacgatt cggctgacgg gtgcaaaaat aagagcctaa cagtttacat
    20461 gacgttaaaa cggagggatg gacatgacaa aaattgatat tatacagaat gtttacgaaa
    20521 aactcggctt ttccaaaaaa gaatcttcag atatcgttga gtccgtattt gacatcatta
    20581 aagacagcct tgccgcgggc gaacgggtga aaatatcggg tttcggcaat ttcatggtaa
    20641 aggaaaagcg cgcgcgccgc ggccgcaatc cccagaccgg cgaggaaata tccattacag
    20701 cgcgccgggt tttgactttc aaatcctccc aggtgctgcg taaatcttta aacagttagt
    20761 gcaacgtttt ttctgcgccg ccggcgccgg aaagactgcc ggctgatttg cggcgggaac
    20821 ctttttgcgg tttttcgcct gttcgtgtat ttctccgcaa gagctcagga atcgtatgga
    20881 cggcatcatt cccgacaaag cctatttccg catcggggaa gtgagcaaaa tcctcaatgt
    20941 tgaaccgtat gtccttcgct actgggagac cgaattcagg accgtcaagc cggtgcgcac
    21001 cagctccgcg caaaggctgt accggaaaaa agacgtggaa gaattgctga tcatccgcaa
    21061 cctgctctac cagcagcgtt ttacgatcaa cggcgccaaa aaacagctca tgaaaatccg
    21121 ctccggagag gaagccccgc ccgaggccgc ttccgaggat aagctcgtcc tgatcaaaaa
    21181 ggagctccag cggattaaaa agataatgag ctgaaaaagc cggctttcgg atttcttctg
    21241 cgaaacaaaa agattacttc ttgttggctc ttttagaagc tttcagcgga ttggtctttt
    21301 gcgtttcctt gatttctatt ttgacgtgcg acgcggtcgg gcagttgccg gtcagccact
    21361 tgcttttggg gtccggatag accttgcagt accggccggt ttcaaattcg aaaattttat
    21421 tgcattctcc gcatttatca acgacaggct gacagctgtt cccgaaaaaa ccgcagccct
    21481 ttttggacat gaaggcgcat tccactccct ttttgactgt ttcgcaaagc atgttgcaca
    21541 aaccccctta cctgaatttt ctgtaatgtt gaacgccgcg gaaagcggcg gccgaactgg
    21601 tcttgaaaac cggcgtgtcc ttaacagcaa aaaaaccggc tgtcaagaaa aaataagaaa
    21661 aattgaagct tgcgggagcc ctgattaaat tgacacaacc ctgcatttat gttagttcct
    21721 tcaccgcgta aaagcaggga aataatcaag atatgcgcga cgggggaagg gaatgaaaaa
    21781 gggctccttg ctgaaaatct tctggaggtc ttttttcatt cacgcggcgc tgaactttcg
    21841 ccgaatgcag aacctcggct ttgcgtacgc gatgatttcg ctgctcagac aacggaaaat
    21901 gcccgatcgc gaagcagagg agatgctggt cagacacctg cagaggttca gcacgcatcc
    21961 ctatctgtcg gcgccgcttc tgggctcgat cctgcggatg gaggaagacc ggccggacgg
    22021 tgaagacgca gcgcccatca cggccgtgaa gcagagcctg atggggccgt atgccgcgat
    22081 cggcgacgct tttttctggg gatcgcttcg gccctgcgcg gggatctgcg ccgtcggact
    22141 ggcctggctg gggtgcgttt ttgcgccgct ggtgtttctc ctgatctatt cgccggcgca
    22201 cctgctcgtg agagggcggg ggttctttga aggctaccgc ggcgggaagc agggaatcgc
    22261 ggcgattcgc gcaatggatc ttccaggcgt gacaggccgc attcgctggc tggccctggc
    22321 ggccctgatc gcgggcagcc tcttggtttt ccaggccggt ttccggggtg cgtctgcgtt
    22381 tggcgttgcg gcggccatgc tcgccgcgct tttgctctgc tggtggctgg tgaaacgggg
    22441 cgtatcgcaa acctacattc tgtatggcgc cgcggcgctt tttatgattt tttcgacaag
    22501 agggttgtgg ctgtgctgga tatgaaaacc tttaccttga aaaacaagct gggcatgcat
    22561 gcgcgcgcgg ccgcgctgtt tgttcggatt gcccagaaat accgctcgga tattgtcatt
    22621 gaacgcaacg ggcagaccgt caacgggaag agcattctcg gaattctgac gctggcctgc
    22681 ccgatgggcg gccagattac cgtcagcgcc aggggcgatg acgccgatgg agcgcttgcc
    22741 gagctggagg cgttgattga aaataaattc ggagaagaat aaaaacagca gatgatgtca
    22801 gaccagggaa agaaaacttt tgttctcaag gggataggcg tttccccggg cgtggtgatc
    22861 ggcaaggttt accggtttga cccgctggac gcgcaaatat cattttacaa gctgcacagc
    22921 gaggatatgg ttccgcggga aattgagcgc ttcaaaaacg cgctgaaaga gtcctcgcgg
    22981 cagcttctgg aaattcagga aaacctgaag aagacaaaag tgacggagcc gctctatatc
    23041 atcgacgtcc acattttgct tctctcggat aaaaaattca tcagccgtac gatcaaatac
    23101 atccgccgca tgggcgtcaa cgcggaatgg gcgctgcgca tgaccctgga ccattacaag
    23161 cagatttttg aaggccttga ggatgtttat atcagcggcc gcatcagcga cgtgcagtat
    23221 gtgatccaga ggattctgcg caatctttcc ggcgaaaaac acgaaattgt ctgggatgtc
    23281 ggacaggaag gcgtcgtgat tgtctcccat gacctgtccc ccgccgacac ggcgcagatg
    23341 aagatggata aaatcgtcgg ttttgccacc gacagcggcg gccggacgtc ccacacggcg
    23401 atcgtggccc gctccatgga attgcccgcc gtcgtcgggc tggataatgt cacccgcttt
    23461 gtccgcaccg gcgacgacat tatcgtggac ggcacgtcgg ggctcgtggt ggtcaatccc
    23521 tatcccgaca tgctcaaaag gtatgaggaa aagaagcgcc actacgatgc cgccaaggat
    23581 gaatatctga aatacgcccg caagccggcc gtgacggtgg atggctacag cgtgaaaatc
    23641 ggttccaata ttgaatttgt cgaagagatc ccttcggcgg tgtcgcacgg ggcggagcac
    23701 gtcgggctgt accggacgga atttctctat atctaccgcg atgaactgcc gacggaagaa
    23761 gatcacttca ataactaccg gcaggtggtc gaggaaaaaa acttttcctg ggcgaccatc
    23821 cgcacgtttg atctgggcgg cgacaagttc accaaatatc agaagcatgc cagggagctg
    23881 aatccgcaga tgggcctgcg ggccatccgc ttctgcctga aggaaatcga gattttcaaa
    23941 acacagcttc gcgcgatctg gcgcgtcagc gcgctgggca gggtgaaaat tttattcccg
    24001 atgatttccg gcatggagga aatccgggaa gccaagcggc tgctggaaga gactcgccgg
    24061 gagctgctgg cccagggggc gccgatcgcc gaccggatgg aaatcggggc gatgattgaa
    24121 gtgccggcgg ccgccgtgat tgccgaccag ctggcgacgg aagtcgatta tttcagcatc
    24181 ggcaccaatg acctgattca gtacgcgctg gcgattgacc gctccaatga aagggtcacc
    24241 tacctctacg agcccctgca tccggccgtt ctgaggctga tcaaacgcat cgtggactcg
    24301 gcgcacaagg ccggcattcg cgtggccatg tgcggagaga tggccggcga tcctctttgc
    24361 tgtccgatcc ttctggggat ggagctggac gaactgagca tgaaccatct ggccattccc
    24421 cgcatcaaga gaatccttca gcagtccagc ctggaggaat cccgaaagct tctggagaag
    24481 gccctgtcct acaacaacgc cgccgaagtt cgggcctgcg tccaggacta catgacccgg
    24541 cgttttcccg atgagattca gaagagggaa ctgtaggcgc cggacgcgaa tcccgggccc
    24601 ggcaaacgaa gagactggag agtatgagcg aaaaaacgcc cacggaaacc ggacggccgg
    24661 aagagaaata ttactccggc tgcatgtatg ccgatcagga tctctggcgc tttcatgccg
    24721 ccaaggccta tctgtcgcgg gtttccatcg ccgacgagga tgtccaggcg ctgaaggatc
    24781 ttgccggcaa gggggccgtg gtttacgcca tcaagcaaag aagcaaactc aacagcctga
    24841 tcctggccga ggtgaccgcg cgcaaaggcc tgcccatgcc ggtatacagc cacggcgtca
    24901 acatgtcttt ttggcagccg ctctccaaga tgatgaagtt cctctggtct tctttcctgc
    24961 gccgcttccg gaaaaacgag atcgcccgcc aggggaagct cgaattcctc gccggaaaag
    25021 tcgccgcccg ggaaagcgtc atcattcatc tgggagagtc ggagtttatc gaaagccgcg
    25081 cctccgagga tgccattgcc ggcctgatga acctgcaggc cggcctcccg tttcccatct
    25141 acatcgtccc ggtgctcgtg gcgtacggcc ggcgacggga gaaggaggat gaaagccttt
    25201 tcaacatcct tttcggccag acggagcaca cgggaacgat gcgccgtctc atcacgttca
    25261 tacgctattc caagcaggcc tttatccttc ccgccgaacc ggttcatctg ccggagtatc
    25321 tgtccgcgaa cagtcttttg accaaaaacg aactcgtaat ggatttgcgc ggagagctga
    25381 ttgaccggat cgaagaggaa aagacttccg tggtcggacc ggcgctgaaa tcgcgcgaag
    25441 ccatgatggg catggtcatg cacgatccga agctgaacgg gttcctggat gaatacgccc
    25501 ggaacaacaa aaaagaccgg gctggcctgg cccgggacgt ccgccgctat ctgaacgaaa
    25561 tcgcgtcgga ttaccgggaa acgttcgtgg aattgtggga caaggttctg acctggctgt
    25621 ggaacacggt gtatgacggc gtgtccatcg actccgaagg gctggccaac gtgcgcaata
    25681 tttccaagaa aatgccgttt gtcattattt cctgccaccg cagccacatc gattatctgc
    25741 tgttgtccta tgttttttac aaaaacaaca ttcagcttcc gtttatcgcc gcgggaaaca
    25801 acctttcttt cttcccgctg gggtatattt tccgacggtc cggcgcgttt tttctgcgga
    25861 ggagctttcg cggcaacgag gtttatggag aggtcttttc caaatatatg gccatgctgc
    25921 ttcaggaagg tcttccgctc gagttcttta tcgagggcgg ccgaagccgc accgggaaaa
    25981 tggtgatgcc caaatacggg cttttgtcca tgattattca ggcctatcag gacaagtatt
    26041 gcgagaatct ggccgcggtg cccgtgtaca tcggctacga ccgcgtgatc gaggaaaagt
    26101 cctatctgca ggagctctcc ggcgcgccga aggtccagga aaataccgtt gaaatcatca
    26161 aatccacgaa aatcctgcgc aaacggtacg gccgcgttta tgtcaacatc ggccaaccga
    26221 tcattttgaa ggactatctg gccgctcagg aaaaatccat ggacgagatg accgtggaag
    26281 agcgccagag cctttaccgg aaaatcggtt acgaggtggt gctggagatc aataaagtct
    26341 ccgtggtcac gccgtttgcg ctggtggcct ccggcctctt gagccatgac cggcgcggca
    26401 tatcccatga ggagttgcgg ggcatcttaa acgaatttta cgaatatctg tccacccggc
    26461 aggtgaaatt tgcagatacc tttgctcacc gggaccgggc gattacggac gccttgaata
    26521 ttttcgacga gacggacatc atttccaaga tagaagccga ggaagaggaa gaggaaatgc
    26581 aggaagtggt ctattccctc accgacgcca gaagactgaa tctggagtat tataaaaaca
    26641 acattcttca ttttttcatt cctctgtgtt ttgtggcgac ttccatggtc aaaaatcctg
    26701 aagactccat gtcgctggcc aaaattatgg gggattaccg gtttttgaag ggacttttgt
    26761 ggaatgagtt tatttttgac gaaaaccggg aggatgccga tgacgtgacg gaggctttga
    26821 cctacctgca ctcgaaatcc atgattctga cctcgaggcg cgacggcgaa gcctgggtgg
    26881 aaatcaaaag caagggggag gtcaggctca aaccgtttgc cggtctgatt cataactacc
    26941 tcgaatctta ctggatcgtt atccgcagcc tggtatatct caagaaaaaa ccgctttcgc
    27001 aaaaagaatg gctggccaag atccgttcac tgggagaccg gatgttccgc aagggggaag
    27061 tcctgcgcgc cgaagccctg tcgcaggcca actatcagaa tgccatcctg tttttggaga
    27121 gcgcggaaat gatcgtatcc caggccgggg aagaaaagag cggcaaaaga gaaaccgttt
    27181 acagcctggc gaacaaccgg gcgcagttgg aagtgctgag gcgacgcctg ttcaaattcc
    27241 tgtaaattcc cgcagaaaag acccctgtag aaaagaattg acaaacgagg gaaaaagtag
    27301 gaaaaaagaa ctgttttttc gtgaatttta ttaaccgatc attcagggag gatgatatgt
    27361 caaaacgcat gaaaatcgtt atagcgtttg tattgattat gcaaatggtc atcccgtatc
    27421 cggtctatgc cgccacggtg ggaagattca cctcggtggt gggcaatgtc atcctgacca
    27481 gggacggcaa agactacaag ccggtggtca attcagccgt caaggaaaag gatatccttg
    27541 taacgggtgc caactcttcc gcggtcatga cttttatcga cgacagtacc attgccctgt
    27601 ctccaaaaac gcgaatgcag gtcagagaat ttggcgtgga gggaaaagcc agaaagggaa
    27661 tcttttcact gtcgattgga aaactggtgg ccaacgtaac caaatttatc ggcgggaaga
    27721 acaagtttga ggttcattcg cccacggccg tctgcggtgt tcgcggcaca ttcttcggag
    27781 tggaagtggc catggcaggg accgtcatga cgacgaccgc gacctgtaca acgggcgccc
    27841 tgtccgtgtc cgctttgtcg gcgaccggcg ccatcatggc gacttccggc attgtcgcgg
    27901 gtcagacggc ggttattacc tctacgggca tcaccgtcgg cgcggcagga gcggcggcag
    27961 gcgcaggcgc agcggcggga accgcaggta caaccggcgc ggcgggaacg ggagcggcag
    28021 ccggtacggc aggcgcaggc gcagcgggcg ctggcgcggc aggagcggcg gcaggtgcag
    28081 gcgcagcggc gggcatcggc gcgggaacga ttgccgcagc ggcagtggtt gcagcggcgg
    28141 cggtcgcggc agtggttgcg acgacgggga ctacggattc tactcccacg catacaacgc
    28201 cggcgcaccc ataagaataa atcttattga atggattcag tccccctttc accggacgtt
    28261 catcgccggt gaaaggggat ttttttatac ccggaaagcg gcggaatgac caaaaaagat
    28321 tctaaacttt tcctgcattt gaaaaaatgg ctggaaaaag cggttctgga ctacggcatg
    28381 attgaagaag gagaccgggt tctggtcggt gtttccggcg gggcggacag cttcgccctt
    28441 ttggatctgc tcggctcgcc gatgattttt gttccgcaat tttcctggac cgccgtatac
    28501 attgacatgg gctttgatcc gggctttgtg gcgtatcgat tgctggaaga atatttccag
    28561 acgcaccaac atccgtatgt aatggaaaaa acagacattg ggacgatttc tcattctgat
    28621 ttcaaccgga aaaatccgtg ctttctgtgt tcccgtctgc gccggaaaag aatttttgaa
    28681 attgcggatg aaaagggctg caataaaatc gcgtttgccc accacaagga cgatatcatt
    28741 gaaacgcttc tcatcaacct gttttacggc cgggaaatca gcaccatgat gccgaatcag
    28801 ccgattttcg acggcaagct tcatattatc cgtccgctgg cctatttgag cgaagcgctg
    28861 gtaaaaaagt acagccgtga gcggcagttc ccggcggtta aaaacgcctg cccgacgagc
    28921 ggaaattcgc gccgaagcta tatcaagaaa ttgctggatc agctcgaaat tgacaatccg
    28981 gacatccgtc acaatattta tacggctatg agccatgtga agccggacta cctcatgccg
    29041 ggaataaaaa aataatcaac gctctttccc tccttttgca gccgcctgaa ggcggtgcga
    29101 ggaggacaga gtgtgtttta acagtggcaa taaacaaaat ttaatgtaga atcaaatcat
    29161 gtcaaaggat aacgcggccc ataaacttat cgcgtccaac aagttcgcaa gagtcaatta
    29221 cgacattgac gacgtctatg aggcgggtat ggtcctgtcc ggcactgaag tgaaggccct
    29281 gcgcgccggg aaggccaatc tcaaagacag ctatgcggtt gtcaaagaca gcgaagtcta
    29341 tctgcgtgat atgcatatca gccactacga tttcggcaac cgggccaacc atgaaccttt
    29401 gcggccgcgc aagctgcttt tgcacaagcg cgagatccgc aaattgtacg gaaaaacccg
    29461 cgaaaaggga ttggcgctgg tgccgctgcg gctgtatttc aaaaacggca tggtcaaggt
    29521 ggaaatcggc gtcggcaagg gaaagaaaat gcacgaccgc cgcgaggaca tcaagcagcg
    29581 ggaagaacgc cgatccatcg cccgggattt caaatccaag cggattagag tttaattttt
    29641 ttatctcttg ccaatgccgc ggcggcgggt aaggaaacga gctgcgaatg acgagatgcc
    29701 tgaggcgagg ttttatgaaa ggcaagaaag tcagcgaaag cagagtgatg atggggcagg
    29761 ggatgaaccc ggaaaacgcc aatcctgccg gcaatgtcca tggcggagac atcatgaaac
    29821 tgattgacac agccggcggc atcgcggcaa cacgccatgc aagggcgcat gtcgtgacgg
    29881 cctccattga ccggatggat ttccatcacc cggtttacat cggggatttt ttaatcctca
    29941 aagcctccgt caattttgtc ggcaagacat ccatggaagt cggcgtccgc gtggaggcgg
    30001 aaaatcccat ttcgggggta aaacgccata ccggctccgc ctatctgacc tttgtggcgc
    30061 tggatgcgaa caaaaagccg atgcagctgc cgccgttgat tctggaaacg gaagaagagc
    30121 ggcttcgcaa cgcggaagcg tccgagagga gacaaatgcg gttggcggaa aagaaaaaag
    30181 agaagaaaaa gcacgaaaag tgagattggc ggttgccgcc gcttttcttt gacaaaccgc
    30241 cttttgagga ttatagtata aacaaataaa aaaagggggg cgtaacggtt tcgacgggga
    30301 catttgaagt tgtagaagcg tgccgaggtt
//
LOCUS       DAOPAF010000016        28904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1767_length_28904_cov_55.166522, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000016 DAOPAF010000000
VERSION     DAOPAF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 28904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28904
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1767_length_28904_cov_55.166522"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4369
                     /locus_tag="PLQ77_03110"
     CDS             <1..4369
                     /locus_tag="PLQ77_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09699.1"
                     /translation="RFNPDESGQTTWSGQTGGIGTFGYYYDSTYGNMGDTGYWLADAS
                     GNWWGDGPVDGALNNGWFITGTKSGNLSGNFLGVYEGQGNTWQATGLGTWSGKPLAYS
                     AKVLSGSWGYYNTTPGSEGLVNDGYPIAGLTGGFSPLFGELNADVMGLGTYTRNQNSP
                     LFNMLLTNADGGPETNPFHVYYGGSWFPAGIDYPEWFEGKMMGFYGRNWDGTKYNEIG
                     LITSGQLIDENGEDSGFSVWGDLYSNAGMWKIPDDEDQGTLTHTTKPGTASSLSVVES
                     YEWSPGAKVDGDLQGTMRTKSAHIDGIEDWGVWMSAMGGSYPDTLPERWASYTGWKET
                     NESGKPSYSIASVDGYAYNDGALWGGVFGRFLNPDNMGVFTGDMVGITNNGNWQAMGI
                     GSFEVTKALAFSSVFSGQTFKTAKGTLNINIYAMPGVNSNMGIFQGVDYETAYFRAEG
                     DTSGSLVKYGWQWENARNRYNNQRTDLIYYPNDSLQSLRVVNGVPVEVQGSTSASSSA
                     WGFWPDLHQSPPVSPIVANSNFIIVNDAFPFTGQLAVNPANMEFAKIFSQQVDNWYTT
                     VRLIPDPEVPLAPTFEGIMGGYAKDGDAGRTLWTSTEDNPMGLALMGEHAEFEAPVTM
                     ATGFGSSYNGTSTTPDGGAYFGFLTNRVSANRSTRGVFYGFYISPESEAGFLKSTNIA
                     GMSFAGLNMWEADGDIYSEMLGTISMPASDLMTNIQLGAMSGNIQYGGAGGNFVSGGY
                     LKHWAMGNGYTASIKDHSSNGIFINASSAGEYSNPEGSTNWQADFAGYGTFGNFGDAG
                     QPGAFSDYGIWHMNAGGADASWAANEIYAPVSGEFLTYTKKGNLSGTFLGNYSGTTSG
                     IWGGTASGVYQKTANVAFSSGMAGKVYANNLTKWISGTNNGTQYWINGDPANFPNERL
                     YVQKTSCMEGACTAVQYADYGYVDEANPVAYTKTTWQYNATEGSITGQTLAESASMSY
                     ENYLAEVNAMRAGAQEYTGWSLNNKNFQGVFAGFNSDSNNLWYNMANGQPTSIELAGL
                     YSDAKGSQAPGIMTTGLASFNPFNAMDPLVNSESPIGGAYYAYMGAAFGKHQVASDLF
                     DGLISGLYLNPDGKAGILYGSIGGYNQPDTGYFHGSGAINGGCQLGEGFNGLSAQNFA
                     YNVIQSSQQWNYGTNDVTVIGDNVKLTTINRNNAWINQNYLSDNGFWGVVQTVRGGTK
                     DSGLAPTAWAWAAHQMNASTPRSDYTGIEITKTENGISTGNYVGAAAIYSGGPVSDPS
                     FTAVSAGSIKGLFDPTATTWQAVSQGSFMETKNFLAKAAAMSGDAAAQEAFMNATKIP
                     AIQVGQATLSQAPNTVVNNLSNVTMNNVAFFAYSTGAVPKIWATNDVRGNYEVIGGYS
                     VAPSTSGAAINNVPLSGNGLTATFNVNHWNGSTWGANVAGSGNLSGGSYAGPVQFQGG
                     AAGTIQSGSFSGTGAGIAK"
     assembly_gap    4508..4607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4680..6125)
                     /locus_tag="PLQ77_03115"
     CDS             complement(4680..6125)
                     /locus_tag="PLQ77_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL09700.1"
                     /translation="MVLRQQNKRIVLVHPRGSNWFPGRRDITDIANRMVPQGMLSIAA
                     WLTRDGHDVFVYDCLGPGAPVDTNRQAHDILAFDPQIVGFSATTSSFPDAADLAQKIK
                     DSRPDTVTVCGGVHVSALREILLAAYPAFDYLAAGEGELTLGELAAGVDPSEIAGLIR
                     RENGIAVANEPRKHITNLDDLPFPAYEKLRGFPRDYHLPLFSYKQTPGATMITSRGCM
                     YSCSYCDRSVFQKGFRYNSAAYIYDHMKYLREKFGARHVNIYDDLFTANRKRIVELCE
                     KLARQPLGMNFNCAVRVGYTDDDLLGMLKEAGCLMVSLGIESADPDMLARHKSGVSLD
                     DVRETVSRIQSAGLRAKGLFMMGLPGETEASIRRTSDFIISLGLDDMNMAKFTPFPGA
                     PLWGAIREEGTFTEDWRLMNCLNFVFIPRGIESKEKLDHLYNEHVKRFYSDPAWRRRF
                     RGRIWEHRHSMLYLLRHLPSFWSAKNQFEPD"
     gene            complement(6303..7424)
                     /locus_tag="PLQ77_03120"
     CDS             complement(6303..7424)
                     /locus_tag="PLQ77_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPL09701.1"
                     /translation="MTEKLKHSSLPVIAAGYLGPALVPNADGAWEEAKLPANPDDLVQ
                     KMMGQPMRRAGRFIKLVCCGALACMSRVPEETLRGRSIGIFLATGLGNIDEILPFITQ
                     VFKHDGGFPSPNQFANSVSNAAAFFLAKACGIKGVVLTVSQEELSFESALWLAQGYLA
                     SGEIDLALVGGCDVFTPSVEDYRERLNLVPGRCRRAPLGEGSAWFLLGGDGEAKIGDI
                     LSVEISSGLADAESLPSDDLLKQCEKLFEEFRNNMESPDNGANSALLSFPRKRESRPS
                     DGLVDSRLRGNDREEGFFEDDKISKVFLPGFRLANLDEASWKKSGWDMDDYLIRCGIS
                     STASACGLARALLSRQSSFLVHGNLSSSGRPALMSAVTK"
     gene            complement(7428..8603)
                     /locus_tag="PLQ77_03125"
     CDS             complement(7428..8603)
                     /locus_tag="PLQ77_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPL09702.1"
                     /translation="MRNGNIHIRGWGAVTALGWGADQGARRLIAGDVHPAGVCGSAHL
                     VNREFKGFEVACDGNPLEKAMKMLDASIAEAFERGGLSAPEIAESALLVGTTGGIFIR
                     NEHEFTESVRRNPGKESPPIVCRNRGPGEVADVIARRYGIRGLPMTFSMACVSSSHAL
                     VVAADLIAQKRIRRAVVVGFEPLLNLTLQGFASLLLYDPDECRPFCNRRMGMQIGEAA
                     SCVILEESDSAAAGKSGFAFAGGHLYNDNKSLTSAGTDGEMVRGVVSRALLKAGTEPE
                     KIVAVKAHGTGTRDNDLSEGRGLAKVFEKHLPPVVSLKATIGHTLGAAGTVETALWLS
                     CLDQGVIPKSFGFAETDPEIGFAPSQENIPAAAGAYLFTYFGFGGTCTAYVIRKMAS"
     gene            complement(8600..8911)
                     /locus_tag="PLQ77_03130"
     CDS             complement(8600..8911)
                     /locus_tag="PLQ77_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09703.1"
                     /translation="MDLEQAYRDLNVSSREDLLFKLKELVIRACEIRDVRPEDVPTDV
                     PFIGGPGPLKLDSLDAMEIAMELRYTFGVELKNASTAAKAMQNFDTLADFVIAAPKVK
                     K"
     gene            complement(8902..10023)
                     /locus_tag="PLQ77_03135"
     CDS             complement(8902..10023)
                     /locus_tag="PLQ77_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09704.1"
                     /translation="MIFKQLIELVRKEMLVLVRDRQTILLLFLMPVALIFFMSLALEG
                     VWADKLVGRKISMVVDNQSRSYQANLLAGKIKAHALIVPVERPAGADVDRIFAEGTAQ
                     AVVTIPPGFEEGDRPVEIRFDPVMDASYRIAARSLITSLAVEVVMGVDNLEAVVSGLV
                     VERSGPNRELPTPLQQTVPAYAIFAMFFIAIPMSVGFLREKKDGTLQRLFTYPVSANL
                     VTLGKITPYFLINVVQFVLMMLVGIFVMSRFIAPAFHAGAHPWHMLPVTLAVAAATTG
                     FGVLVAATAKTPEQSSTLAATGAILMGVFGGIMVPHAVMPPVMKKLAMISPMFWAHQA
                     YLDIFLRDAAFGIILPRLIILTLFAIICFYVAGRKVRWI"
     gene            complement(10013..10732)
                     /locus_tag="PLQ77_03140"
     CDS             complement(10013..10732)
                     /locus_tag="PLQ77_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09705.1"
                     /translation="MNALDISSVTFCYDGSPAPALRDFSMAVADGRTHALLGPNGAGK
                     STLMRIITGQLQGQTGGVSVYGLRMPDSKALSFIGCAPQPISLYPSLTARENLRFFGA
                     MAGMSKDQIASRSDWALEKTGLAESSEAAVASFSGGMQRRLNLAAALLHSPKLLLLDE
                     PSAGVDPQSRNHMYDLLMELNAGGVTILLCTHMMEEAQRLCSGLTLLDRGERIYDGRM
                     SDIGNLEEFFLKKTGRGLRDL"
     gene            complement(10729..11763)
                     /locus_tag="PLQ77_03145"
     CDS             complement(10729..11763)
                     /locus_tag="PLQ77_03145"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPL09706.1"
                     /translation="MTTEVTAAGKPFIHVHSAHCESGVTAALFRDKGVEISEPMIFGI
                     GSGIFFGHLPFVKMGGVTISTFRSQPGAVFRKSAKRLGGKFKTARFRNPKKGMDELRR
                     VLGTGQIVGLTTNIYWLSYFPNRFRFQFNGHNIVVLHEIENGFRVSDPMLEEPVDCPA
                     EDLERARFARGPLESRGFMYYPQSVNPNPDLRAAGLQGMKDTCKMMLKIPLPIFGIRG
                     MRYLAKKIIRSPEKLGFMGACRELSSVVRMQEEVGTGGAGFRYMYAAFLQEAAELFAS
                     KELARLSGEMTAIGDIWREFAVVSARIIKKRGQTEETFEKAGGLMLICAGREEQLFKD
                     LAKAAGKLKA"
     gene            complement(11783..12181)
                     /locus_tag="PLQ77_03150"
     CDS             complement(11783..12181)
                     /locus_tag="PLQ77_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09707.1"
                     /translation="MNVREVPQDITYYEGKKRACWALNDEGKYVIVPSSGWEVEAVVN
                     GLAVEEMAASLEATRQAVLAGVKSPLCYYMELRQMTPEILGKTARVAAFRVRRHFRPD
                     VFSKLGPSVLDRYARALAVTLDELKTVPQT"
     gene            complement(12266..13147)
                     /locus_tag="PLQ77_03155"
     CDS             complement(12266..13147)
                     /locus_tag="PLQ77_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL09708.1"
                     /translation="MKKLFYKISIYLSRRLGLWFFRVFSWFIATGYFFLFPPRVRDSL
                     QFYRALFPGRSFGYHLYCVWKQFHNFTGVYVHRFIPWAAEEAKFTREGWEHLDEAVAK
                     KTGAIVVMSHIGNWELAAQRLQEKGLPVMLYLGAKHKEQIEKMQKEKLAQTGIRIVTT
                     DEKEKSPFALLEGVGFLREGGIVSMAGDRLWGDQTSVEVDFLEHKVRLPDTPHLFALM
                     SGAPLMTFFVHEKAPGHYHVQVSKGRKVAAAARAERKKAVQESVQAYADDLARFAAAH
                     PFEWHHFEPFLGEKIIP"
     gene            13365..14087
                     /locus_tag="PLQ77_03160"
     CDS             13365..14087
                     /locus_tag="PLQ77_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPL09709.1"
                     /translation="MTAKKVLAQPVSRARLNEEIVTVLQNQILSGKLAPGDKIPPERE
                     LAETFQVNRATLREALRKLEILELVEIRHGNGLYVKNYLDSGNLDLIRAAANLDGREE
                     VVLDILEARRYLMPQIAWLAAARRSNEDLLELERVTGSEELTMLERDIKVHQIIARST
                     HNLLCTIGLNFFNQIFRDYGPLYFNDEKNAQRSRKFHRDIYEAIKSQKPIEARKIMRE
                     IMIYAEEVVKAGLSGNKKQKES"
     gene            14090..15736
                     /locus_tag="PLQ77_03165"
     CDS             14090..15736
                     /locus_tag="PLQ77_03165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPL09710.1"
                     /translation="MLRFIENYTNEKFDVLVVGGGITGASVAYEAAARGLKVALLEKK
                     DFSWATSSVTSKLIHGGLRYLANGEIGLVRESLRERRVLENIAPNFVYPIPILMTHYK
                     KPLKNSKWVVKAGMLMYDALSYDKKYTWDASKKIPRHKTISRRTVLESEKNVRPAGLT
                     GASLFYDCMSIFPERLTLAFIKSAAACGAKVANYAKVESFLTDAQKNVAGVRVKDLLT
                     GKTMAISSSVTINCAGPWADLLLGLAESNSAGGAALRRSEGIHIITKKKLVSGKYVVA
                     CMTPKGRHFFLIPWRGHTIIGTTDKPFVGNPDAYHVTKAGILELIDEVNGSFGDGTLS
                     YADVVHTYGGLRPLVEGQTEETYTSSRRYEILDNKENGLNGLITAEGGKFTTSRNLAE
                     KCVDLVEAKLGRPRAKSKTARHHLAGCRIKNLNFFMEEIHQHNTNGFCRNTLDYLGRC
                     YGTDYRAITKLAHENKELAEQINEDGEIFAEVVYAVREEMARTLSDIVLRRTGIATLG
                     NPGEKILRKVAVVAAKELQWTPERMEREIAGTINTLRLPQ"
     gene            15766..17478
                     /locus_tag="PLQ77_03170"
     CDS             15766..17478
                     /locus_tag="PLQ77_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPL09711.1"
                     /translation="MTATNFRPDWTEKAPPAGTYRSIFKYGNPNHFKHPSDAWYAMLK
                     QDFHLTDEDFSAKSGEGLNLVSLNRPSALKPGQIEALRAIAGVENVACDDYSRLKYSS
                     GKTTEEMLELREGIVREVADVVVHPRDKHDVRKIVEYCDRELIPVTVFSGGSSVNFGC
                     RPSRGGVSLAMSTHMNKLLEINEINQTARMQPGMFGPACEDALNRAPQLWQTKHRYTC
                     GHFPQSFEYSTVGGWVATLGSGQASTYYGDACDIVFSQEYVTPAGTFKTLDYPATATG
                     PKVNDIMKGSEGAFGILVEVTMKIFRYLPENRTYFSFMYPSWEKAVEASREIMQSEFG
                     RPAVYRISDPEETDRGLKLYGIPDLADRFLTLRGFKPLCRCLCLGNCEGDKDYSRLVA
                     KKIKSTARRHGAVSLSGYAARKWEQSRYTEPYMREDLGDYGILIDTLEAAVTWDNLHR
                     LHQGVRAYIKSRPDTMCMTHASHFYPQGTNLYFIFIIRMNDPGEFRKFQRGIIDSIQK
                     YGGSLSHHHGIGRMMAPWMETHLGKEQMAVLRALKNHFDPRGIMNPGGLLELKSSGEK
                     RWKT"
     gene            17466..19052
                     /locus_tag="PLQ77_03175"
     CDS             17466..19052
                     /locus_tag="PLQ77_03175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPL09712.1"
                     /translation="MENVKKPSGEMILSFDAGTQSIRAALIDASGNIAQIVKRRIEPY
                     FSPEPGWAEQHADYYWKMFCEACRELLDKSDKSSREAIVAVTLTTQRATMINLGEDGR
                     PLRPACLWLDQRKANAEEIVPSFAKPLLKSIGLWDRLNNAVQSCPSNWIQQHQPEIWR
                     QTHKYLLLSGFFTFMLTGEYADSAACNVGYLPFDNKTYQWAGRFDLKWRLFPIEKEKL
                     PRLVKPCEVLGRITARAGEETGIPAGLPVIAAGTDKGCEMLGAGCLTPDTGCLSFGTT
                     ATFNTTTTKYIELLSMIPPYPASVPDTYYTEVMIYRGFWMVSWFRDEFGMLERKIAEK
                     NNIAPEKLFDQLIEKVPPGSMGLILQPYWSPGTNTDPYAKGSIIGFGDVHNRAYLYRA
                     ILEGLVYGLREGAELTQKRTGTAVSRLRVSGGGSQSDMAMRIAADIFGLPAERPHTYE
                     TSALGAAIDAAVGLGLYPDFPSAVKAMTRVKEVFEPVAENHRIYDRLYREVYLAMYGR
                     LRPLMEKIQDITGYPSPSRQ"
     gene            complement(19092..19316)
                     /locus_tag="PLQ77_03180"
     CDS             complement(19092..19316)
                     /locus_tag="PLQ77_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09713.1"
                     /translation="MASGKNPEGRRRKFTYDEIVARDKTSLDIAWLKDKSLADLNNLP
                     HPDALANDIIENLESAVASFKEIMETINGK"
     gene            complement(19384..19959)
                     /locus_tag="PLQ77_03185"
     CDS             complement(19384..19959)
                     /locus_tag="PLQ77_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPL09714.1"
                     /translation="MTEQNAQPQPLKTIADPACGTGGFFLAVYDCISDPQNFILNKEQ
                     KLFLKNSTYYGNEIVAAARRLALMNMFLHNIGDIDAETFILPTDSLVADTGLRVDYVL
                     TNPPFGKKSSMTFTNEEGEQEKDDLTYNRQDFWATTSNKQLNFVQHIRTMLKTTGKAS
                     VVVPDNVLFEGGAGEIVRKKLLETTDLHTIL"
     gene            complement(20056..20406)
                     /locus_tag="PLQ77_03190"
     CDS             complement(20056..20406)
                     /locus_tag="PLQ77_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09715.1"
                     /translation="MKKLFYKISIYLSRRLGLWFFRVFSWFIATGYFFLFPPRVRDSL
                     QFYRALFPGRSFGYHLYCVWKQFHNFTGVYVHRFIPWAAEEAKFTREGWEHLDEAVAK
                     KTGAIVVMSHIGNW"
     gene            20703..21203
                     /gene="ectA"
                     /locus_tag="PLQ77_03195"
     CDS             20703..21203
                     /gene="ectA"
                     /locus_tag="PLQ77_03195"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HPL09716.1"
                     /translation="MKKDPTDMYFRRPEVADAPFIEKLVKDSPPLDLNSLYCYLILCA
                     HFSRTSVVACDADAVCGFISAYLRPDEEDTLFVWQVAVDKKYRGKGVARSMLSSILNR
                     PTPCPPRYLETTVSPSNIPSKALFSSLAASLGVPLVQSVLFSADDFGDQRHEEEVLFR
                     IGPFDL"
     gene            21221..22474
                     /gene="ectB"
                     /locus_tag="PLQ77_03200"
     CDS             21221..22474
                     /gene="ectB"
                     /locus_tag="PLQ77_03200"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: protein motif:HMM:NF006733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HPL09717.1"
                     /translation="MKTIRRLESEVRSYSRSFPVLFEKAKDAFLFDADGNRYIDFFAG
                     AGTLNYGHNNDHMKKKLIEYLETDGIVHGLDMATVAKEELLLKMESIVFKPRNLDYKI
                     QFTGPTGTNAVEAGLKLARMVKKRSNVISFTNGYHGLTMGSMSVTANAYYRDEAFINR
                     SDVSFLPYDGYLGKDVNTAQYMRRFLEDNSSGVDIPSAVILETVQGEGGIRVASDEWL
                     REVESICREFDILLMVDDIQVGNGRTGHFFSFERSGIKPDIVTVSKSFSGYGLPMSLV
                     LFRRELDIWKAGQHTGTFRGNNLAFVSASVALDYWKDDRFSKDIFRKGTILSDRLHAI
                     CARYPQLNAEVRGRGFIYGMAVDPPEIAREISMECFKNKLIMELAGARNNVIKFLAPL
                     IIPDEVLMEGLDILDASIGSVLKNR"
     gene            22490..22900
                     /locus_tag="PLQ77_03205"
     CDS             22490..22900
                     /locus_tag="PLQ77_03205"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HPL09718.1"
                     /translation="MLVRTINDITGTDREVWAENKNWVSRRLLLKKDGMGFSFHETII
                     FAGTQTHIWYKNHLEAVFCVEGEGEVETPADGKIHHIVPGTIYALNNHDEHLLRASKD
                     MRLLCVFNPPVTGAEVHLEDGSYPPAENQGKVPS"
     gene            complement(22997..24712)
                     /locus_tag="PLQ77_03210"
     CDS             complement(22997..24712)
                     /locus_tag="PLQ77_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL09719.1"
                     /translation="MYEQKPWLKFYGNVPHSIDYPRVTMYEALLQTAREKADAVAYDF
                     MGHASTYRGFISEIDRCADALAALGLKQGDRITISMPTSPQGIIAFYAANKLGAVASM
                     IHPLSTAKEIEFYLNVAKSRFALTLDAFYAKFKEVKAATPLQTLILARIPDYLSFFKR
                     VGFNLTKGRQIPKVPADPMVKWWADLMKGGYPSAPPARMDTDDMAVILYSGGTTGVPK
                     GIMLSNMNFISEGKMVSEWGKLDDNASVLAILPIFHGFGLGVCINACFMGGGKSILVP
                     VFTPETVADLIRSRKPSFVIGVPTLFDALSRNPKMHKADLSCLRATFSGADTLPRPVK
                     ERFEEMVRKQGGNVQLLEGYGLTEAVTAIMATPIGCYREGSIGVPFPDMLAKIVKLDT
                     IEEAPLGEEGEICVAGPAVMMGYLDQPEETAATLRKHADGRIWLHTGDIGTMDADGFF
                     YFKLRQKRMIKSSGMNVYPAQVEELLYKHDKVRDACVIGVPDEAQVQAVKAFVVLKNP
                     ADAGPAAEKELIHYCRENLIKWSCPRSIEFRTDLPKTLVGKIAFNTLEKEEVEKLKKE
                     GKFAG"
     gene            complement(24809..27877)
                     /locus_tag="PLQ77_03215"
     CDS             complement(24809..27877)
                     /locus_tag="PLQ77_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPL09720.1"
                     /translation="MPPRRYPIRWSMVLVTALIVGILFCWETSRLKIETDILESMPHH
                     DPVLASARRVITHLPVSDRLFIDLEQTSSDRDQLVEAARLVEDRLSKSGLFERVGIAD
                     EAGAFPELMAHVREHLPSLFGTEDLERKIAPLLAPEKIRETMAENRISLGELQGIGRA
                     EMISRDPLGFSPVILERMSSLSPAGSAQFYSGRLLSSDGRHALIIARLAGSGTDTTRA
                     AQISGLMDDCARMLEARRDSNGRFTLTPVGAYRAALDNETVARRDTRLAVLLTTLGIA
                     LLLILSFPRPLIGLLALLPSAVGAIAALFACSFLFKSMSMLAVGFGGAIMAFTVDLGL
                     TYLLFLDQPRATFGREAAREVWSAEFTAALTTICAFLLLLISDFDILAQIGVFAALGI
                     TFAFLFVHFVFPIIFPEMPPARRASNRLLTGAVQKIAAPASWKLIAALVFGLTMLLFA
                     KPVFNVDLQAMNSVSSATLAAEKKLQDTWGNLSGRLYVLLEAPDLERLRKKNDQLEAL
                     MAREVKGGRLAPAFLPTVLFPTPESAARHFAAWRSFWTPERVGALRTNLEKAAAENGF
                     APDAFDPFWTVFQAQRPGVFTIPPKHLEMLGIARTSEGFAQLTPMAAGKHYNASEFFD
                     RLAAAGEIDLFDADFFNRRMGEFLKSIFIEIALIVSLGLIAVVFLFFLDWRLSLAVLA
                     PVAFALISTLGTLKLIGRPLDIPGIMLWVVILGMGVDYAIYYTCTYQRHPEEASPAMN
                     TVKLSIFLSAATTFIGFGVLTLANHSLLRSIGLVSLLGIGYSLLGAYFILPTLMRKIF
                     SPFDFPAGAVQPASREHVRRVLLRYRLLPGYPRVFARFKMKLDPMFSELHRYVQNPRR
                     MLDIGCGYGVPAAWLLELHPEAKVYGLEPDEERALIARRVIGARGDVQVGRAPDLPDV
                     EGSVDHVLMLDMLHLIDDRELQLVFQRIHDKLGHDGMLVIRATVPSDRKVPWKRWIET
                     ARLKWTGIPERFRGEREIAGFMEAAGLTVRVHASPVPGVEEKWFVGGKGY"
     gene            complement(27993..28766)
                     /locus_tag="PLQ77_03220"
     CDS             complement(27993..28766)
                     /locus_tag="PLQ77_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL09721.1"
                     /translation="MSHKKRTANEQKDPKSRRRAGRNLGRFAFVIPAYNHAATVAQVA
                     GRARDMGFPVFVVDDGSTDDTSRRLAEVQGIEVLRHEKNEGKGAAILTGFMAASHKAD
                     YAITIDADGQHDPEDAIHLIDAIPLKKRPIVVGAREGMDAVQGAHIPWTSRFGRKFSN
                     FWVRMSGGPKISDTQSGMRIYPLPESLSLPVRARRFQFEVEILVQARRIGIPIIEAPI
                     RVLYLPDGARISHFRPFVDFLRNAKTFTRLMFTRVFGRH"
BASE COUNT         6632 a         8163 c         7980 g         6029 t
ORIGIN      
        1 ccgtttcaac ccggatgaga gcggtcaaac cacatggtcc gggcagaccg gcggcattgg
       61 aacatttgga tattactatg attccactta cggcaatatg ggcgataccg gctactggct
      121 cgccgatgcc tccggaaact ggtggggcga cggcccggtt gacggcgcgc tgaataatgg
      181 ctggttcata accggcacaa aatcgggcaa tctgtccggc aacttcctgg gcgtctacga
      241 aggacaggga aacacctggc aggccacggg actcggaacc tggtcaggaa agccgctggc
      301 ctacagcgcc aaagtcttat ccggaagctg gggatattac aatacaactc caggctcgga
      361 aggactggtt aatgacggtt atccgattgc aggcctgaca ggcgggttct ccccgctgtt
      421 tggcgaattg aatgctgatg tgatgggatt gggaacctac acgcggaatc aaaattcacc
      481 gctgttcaat atgttgctca cgaacgcgga cggcgggccg gagacgaatc ccttccatgt
      541 ctattatggc ggcagctggt tcccggcggg catagattac cccgaatggt tcgaaggcaa
      601 gatgatgggc ttctacggcc gcaactggga cggcacaaaa tacaacgaaa tcggcctgat
      661 cacctccggt cagttgatcg atgagaatgg agaagacagc ggattctccg tctggggcga
      721 tctctattcc aacgccggca tgtggaagat tcccgacgac gaagatcagg ggaccttgac
      781 gcataccacc aaacccggaa cagcctcttc gttatcggtt gtagagtcgt acgaatggtc
      841 gcccggggcc aaagtggatg gcgatctcca gggcacaatg cgcacgaaga gcgcgcacat
      901 tgatggaatc gaagattggg gtgtctggat gtcggccatg ggcggttcct acccggatac
      961 gcttcccgaa agatgggcaa gctacacggg ctggaaggaa accaacgaat ccgggaaacc
     1021 aagctattcc atcgcatccg tggacggata tgcttacaat gacggggctc tctggggcgg
     1081 cgtcttcggg cggttcctga accccgacaa tatgggggtc ttcaccggag acatggtggg
     1141 aatcacgaat aacggcaact ggcaggccat gggcatcggc agttttgaag tcaccaaggc
     1201 gcttgcgttc agcagcgttt tctccggcca gacttttaaa accgcaaaag gcacgctcaa
     1261 tatcaacatc tatgccatgc cgggcgttaa ttccaatatg gggattttcc agggcgtgga
     1321 ttatgaaaca gcgtatttcc gggcggaggg cgacaccagc ggctcgttgg tcaaatacgg
     1381 ctggcagtgg gaaaatgctc gcaaccgtta caataaccag cgcacggatt tgatctatta
     1441 tcccaatgat tcattgcaaa gcttgagggt tgtcaatgga gtccctgtag aagtccaggg
     1501 ttcgacatca gcgtcaagtt cagcatgggg tttctggccg gatctccacc agtcgccgcc
     1561 ggtatcaccg atcgttgcaa acagtaattt tatcatcgtc aacgacgcgt ttccgttcac
     1621 cggtcagctg gctgtaaatc cggcaaacat ggaattcgca aaaatctttt cgcaacaggt
     1681 agataactgg tacacaaccg tgcgcctgat tcccgatcct gaagttcctc tggcgcccac
     1741 ctttgaaggc atcatgggcg gatacgccaa agacggagat gcgggaagaa cgctctggac
     1801 atcaaccgaa gacaatccca tgggtcttgc cctcatggga gagcatgcgg aatttgaagc
     1861 tccggtcacg atggcaaccg gcttcggaag ttcctataac ggaacaagca ccactccgga
     1921 cggcggcgct tatttcgggt ttttgaccaa cagagtttcc gccaaccgct cgacccgtgg
     1981 cgtgttttac ggtttttaca tcagtccgga aagcgaagcc ggtttcctga aatcgaccaa
     2041 tatcgcggga atgtcctttg ccggacttaa tatgtgggaa gcggacggtg acatttacag
     2101 cgaaatgctt ggcactatca gtatgccggc ttctgacctg atgacgaaca tacagctcgg
     2161 cgccatgtcc ggcaacatcc aatacggcgg tgcaggcggc aattttgtct cgggcggata
     2221 tttgaagcat tgggcaatgg gcaatggcta tacagcctcc attaaagacc attcgtccaa
     2281 cggcatcttc atcaatgcat cttccgccgg agaatacagc aatccggaag gctccacaaa
     2341 ctggcaggcc gactttgcgg gctacggcac tttcggcaac ttcggcgatg ccggccagcc
     2401 cggcgctttc tccgactacg gcatatggca catgaacgcg ggcggcgcgg acgccagttg
     2461 ggccgcgaac gaaatttacg cccccgtctc gggagaattt ctgacctata ccaaaaaggg
     2521 caatctgtcc ggaacgttcc tcggcaacta ttccggaacg acatcaggca tctggggagg
     2581 aaccgccagc ggcgtctatc agaaaaccgc caatgtcgct ttcagcagcg gaatggcggg
     2641 aaaagtgtac gccaacaatc tcaccaagtg gatatcaggc accaataacg gtactcagta
     2701 ctggatcaac ggcgatccgg ccaatttccc gaatgagcgc ctgtatgtcc agaaaacgtc
     2761 atgcatggaa ggagcatgca cggctgtgca atatgctgat tacggctacg ttgacgaagc
     2821 caatccggtg gcctacacaa aaacaacctg gcaatacaat gccacggaag gttcgatcac
     2881 cggccaaacg cttgcggaat cggcatccat gtcttatgag aattatcttg ctgaagttaa
     2941 cgccatgcgc gcaggcgccc aggaatatac cggctggtct ttgaacaaca agaacttcca
     3001 gggcgtcttt gccggtttca attcggacag caataacctc tggtataata tggccaacgg
     3061 gcagcccaca tcgatcgaac tggccggtct ttacagtgac gcaaagggca gccaagcccc
     3121 cggtattatg acaaccggtt tggccagttt caatcctttc aatgccatgg atccgctggt
     3181 taattcggaa tccccgatcg gcggcgctta ttatgcctac atgggagcgg cctttggcaa
     3241 gcatcaagtt gcgtccgatc tgttcgatgg cctgatttcc ggtctctatc tcaatccgga
     3301 tggaaaggcc ggaattcttt acggaagcat aggcggttat aaccagcccg atacgggcta
     3361 cttccatggc tccggcgcca tcaacggagg ctgccagctt ggcgaaggat tcaacggcct
     3421 gagcgctcag aactttgcgt acaatgtaat ccaatcttcc caacagtgga attacggaac
     3481 aaacgacgta accgtaattg gagacaacgt aaagctgacg accatcaaca gaaacaacgc
     3541 ctggattaat cagaattatc tctccgacaa cggcttctgg ggcgtcgtcc aaaccgtaag
     3601 gggggggacc aaggattcgg gtcttgctcc gaccgcctgg gcatgggcgg ctcaccagat
     3661 gaatgcctct acaccgcgat cagactatac gggcattgaa atcaccaaaa cggaaaacgg
     3721 catcagcacc ggcaactacg tgggcgcggc cgcgatttat tccggcggtc cagtctccga
     3781 tccgagcttt acggccgtca gcgccggaag catcaagggg ctcttcgacc cgaccgccac
     3841 cacctggcag gcggtatccc agggcagctt catggaaacg aagaacttcc tggcaaaagc
     3901 cgccgccatg agcggtgacg cggcagccca ggaggcgttc atgaacgcca caaagattcc
     3961 ggcaattcag gtgggtcagg caactttgag ccaggccccc aacaccgtgg taaacaacct
     4021 gagcaatgta accatgaaca atgtggcctt ctttgcctat tcgacgggcg ccgtcccgaa
     4081 aatctgggcg accaacgacg taagaggcaa ttatgaagta atcggcggtt attcggtcgc
     4141 gccgtcaacc agcggcgcgg ccataaacaa cgtgccattg agcggcaacg gccttaccgc
     4201 aaccttcaac gtcaatcact ggaacggcag cacctggggc gccaatgttg cgggaagcgg
     4261 aaatctgagc ggcggttcct atgcgggacc ggttcagttc cagggaggcg cagcgggaac
     4321 tattcaatct ggatcattct ccggcacagg cgcggggata gccaaataaa gcggcgtgta
     4381 tgaaaaaggc atgactttgt aaatttattt aaacatcaac caaaaaggga ggcgcgtgat
     4441 tcacgcgcct cccttttttc gttcattacc ttctagttcg aagggtcacg gttcgacagg
     4501 ctcaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ttttcgttca
     4621 ttaccttcta gttcgaaggg tcacggttcg acaggctcac catgacacag aaaaaggctc
     4681 tagtctggtt caaactggtt tttggccgac cagaaggagg gaagatgcct aagcagatac
     4741 agcatgctgt ggcggtgctc ccagatgcgg ccccggaacc gcctgcgcca ggccggatcg
     4801 gaataaaagc gtttgacgtg ctcgttgtac aggtgatcga gtttttcctt ggattcgatt
     4861 ccgcgcggaa taaaaacaaa attcaggcag ttcatcagcc gccaatcctc cgtgaaggtt
     4921 ccttcctcac ggatcgcccc ccacagcggc gcgccgggaa agggcgtgaa cttggccatg
     4981 ttcatgtcat cgagccccag cgaaataatg aaatccgacg tgcgtcgaat cgacgcttcg
     5041 gtctcccccg ggagccccat catgaaaagc cctttggcgc gcagtccggc cgactgaatc
     5101 cggcttacgg tttcgcgcac atcgtcaagc gatacgccgg acttgtggcg ggccaacatg
     5161 tccggatcgg ccgattcgat tcccaaggac accatcaggc agcccgcctc tttgagcatt
     5221 ccgagcagat catcatccgt gtagcccacg cgcacggcgc agttgaagtt catgcccagc
     5281 ggctgtcgcg caagcttttc gcagagttcc acaatccgct tgcggttggc agtgaagaga
     5341 tcgtcgtaaa tattgacgtg gcgggcgccg aatttttcgc gcaggtattt catgtggtcg
     5401 taaatatagg ccgccgagtt gtagcggaaa cccttttgaa aaacggagcg gtcgcagtag
     5461 gagcaggaat acatgcagcc gcgcgaagtg atcatcgtcg cgccgggggt ttgtttgtag
     5521 ctgaaaagcg gcaggtggta gtcgcgggga aacccgcgga gtttttcata ggcgggaaac
     5581 ggcagatcat cgaggttcgt aatatgcttt ctcggttcgt tggcgacggc aatgccgttt
     5641 tcccggcgga tcagtcccgc aatttccgag ggatcaacac ctgctgccag ttcccccagg
     5701 gtcagttcgc cttcacccgc cgccagataa tcgaaagcgg gataagccgc cagaagaatt
     5761 tcgcgcaggg ccgatacatg cacgccgccg cagaccgtga cagtgtcggg ccggctgtct
     5821 ttaatttttt gcgcaaggtc ggccgcgtcg gggaacgacg acgtggtggc ggaaaaaccg
     5881 actatttgcg ggtcgaacgc cagaatatcg tgagcctgcc ggttcgtgtc caccggcgct
     5941 ccgggcccca ggcagtcata gacaaaaaca tcgtgcccat cgcgcgtcag ccaggccgcg
     6001 atggagagca ttccctgcgg caccatacgg ttggcaatgt cggtgatgtc gcgcctgccg
     6061 ggaaaccagt tggacccgcg gggatgaacc agaacgatgc gtttgttttg ttgacgtaac
     6121 accatgattt atttgtccct cgcgacgcca cacgggcatg aaaataatca gtgcgccgag
     6181 gttattagcg ggtctaaaga cccgccctac atttttttat gtagtgcgta cctcgcgtga
     6241 ccttcaggtt attaggtgcg cttgcttctt tgccttggtc ctactatcgt aggaatgact
     6301 ggctattttg ttaccgctga catcagcgcc ggccttccgg acgaggacaa atttccatga
     6361 accagaaacg agctttggcg ggaaagcagc gcccgcgcca gtccgcaggc ggaagcggtg
     6421 gaggaaatgc cgcagcggat caaataatca tccatgtccc agccgctctt tttccacgac
     6481 gcttcgtcga ggttcgcaag gcgaaatccg ggcaggaaca ctttgctgat tttatcgtct
     6541 tcgaaaaacc cttcttctct gtcattcccg cgtaggcggg aatctaccag accatcagag
     6601 ggcctggatt cccgctttcg cgggaatgac aacaaggctg agttggcgcc attgtccggg
     6661 gattccatgt tgttccgaaa ttcttcaaac aatttttcac attgtttcag gagatcgtct
     6721 gatggcagac tttctgcgtc cgctaacccg ctcgaaattt caacagacag aatatctcct
     6781 attttcgctt ctccgtcacc acccagcaaa aaccaggcac tgccttcgcc caggggagcc
     6841 cgccgacagc ggccgggaac caggttcaac cgctcccggt agtcttccac ggacggcgtg
     6901 aagacgtcgc agcctccgac cagcgccagg tcgatctcgc ctgacgccag gtagccctgc
     6961 gccagccaca gggcggattc aaaagaaagc tcttcctgcg agacggtgag aacaacgccc
     7021 ttgatcccac aggccttcgc caggaaaaag gcggcggcgt tgctgacgga attggcaaac
     7081 tggttggggc tgggaaaccc gccgtcgtgt ttgaatacct gcgtgatgaa cggcaggatt
     7141 tcatcgatgt tgcccagacc ggtggctaga aaaatgccga tgctccgccc ccgcagcgtt
     7201 tcctcgggaa cgcgtgacat gcaggccagc gcgccgcagc agaccagctt gatgaaccgt
     7261 cccgcgcgcc gcatgggctg ccccatcatt ttctgcacca gatcgtcggg attcgccggc
     7321 agtttcgcct cctcccacgc gccgtccgca ttgggaacca gcgcgggccc aagatagccg
     7381 gccgcgatga cgggcaggga agaatgtttt aatttttcag tcatatttta agatgccatt
     7441 ttcctgataa cataagccgt gcaggttccg ccgaagccga aataggtaaa caggtacgcc
     7501 ccggcggcag ccggaatatt ttcctgcgac ggagcaaaac cgatttcagg gtccgtttcg
     7561 gcaaagccga agctcttcgg aatcacacct tggtccaggc aggagagcca cagagccgtc
     7621 tcgaccgtcc cggccgcacc cagcgtgtgg ccgatggtgg ccttgagtga gacgacgggc
     7681 ggcagatgct tctcaaagac cttcgccagc ccccttccct cggaaagatc attgtctctc
     7741 gtgcccgtac cgtgcgcttt gacggcgaca attttctccg gctccgttcc ggctttcaaa
     7801 agcgcccggg aaacgacgcc cctcaccatt tcgccgtccg tgccggcgga ggtcaggctt
     7861 ttgttgtcgt tatacagatg gccgccggca aatgcaaagc cgcttttgcc cgcggcggcg
     7921 ctatcgcttt cttccaggat cacgcaggag gccgcctcgc cgatctgcat gcccatgcga
     7981 cggttgcaga aggggcggca ctcgtccgga tcatacagga gcaggcttgc aaaaccctgc
     8041 agggtcagat tcaaaagcgg ttcaaaacct accacgacgg cgcgtctgat tcttttctgc
     8101 gcgatcagat ccgccgccac gacaagcgcg tgcgaactcg aaacgcaggc catgctgaag
     8161 gtcatgggca ggccgcgaat gccgtatctt cgggcgatca cgtcggccac ttcgcccggc
     8221 ccccggttgc ggcagacaat cggcggcgat tccttgccgg gattccggcg cacggattcg
     8281 gtaaactcat gctcgttgcg aatgaagata ccgccggtgg tgcccacgag cagagcggac
     8341 tcggcaattt cgggcgcgga aaggcctcct cgctcaaagg cctcggctat cgacgcatcc
     8401 agcattttca tcgccttttc caggggattg ccgtcacagg cgacttcaaa tcccttgaac
     8461 tcccggttga ccaggtgggc agacccgcag acgcccgcgg gatggacatc gcccgcgatc
     8521 aggcggcggg cgccctggtc cgcgccccag cccagcgccg tgacggcgcc ccatccgcga
     8581 atatggatat tgccgtttct cactttttca ccttcggcgc ggcgatcaca aaatcagcca
     8641 gcgtatcgaa gttctgcatc gccttcgcgg ccgtggacgc gtttttcagc tccacgccga
     8701 acgtgtagcg cagttccatc gcgatttcca tcgcgtcgag cgagtcgagc ttgagcggcc
     8761 cgggtccgcc gataaacggc acgtcggtgg gcacatcctc gggccgcaca tcccggattt
     8821 cgcaggcccg aatgaccagt tccttgagtt tgaaaaggag atcctcccgc gacgaaacat
     8881 tgagatccct gtatgcctgt tctaaatcca tcgaaccttc ctccccgcga cataaaagca
     8941 gattatcgca aacagtgtca agatgattaa cctgggcagg atgatcccaa acgcggcatc
     9001 ccgcagaaaa atatccagat aggcctgatg cgcccagaac atcggcgaaa tcatggccag
     9061 ttttttcatc accggcggca tcaccgcgtg cggcaccatg atgccgccga agacgcccat
     9121 caaaatcgcc cccgtcgccg ccagcgttga agactgctcg ggcgttttgg ccgtcgccgc
     9181 caccagcacg ccgaaccccg tcgtggcggc cgcaaccgcc agcgtcaccg gcagcatatg
     9241 ccagggatgc gctcccgcgt gaaacgccgg agcaatgaag cgcgacatga caaaaatccc
     9301 caccagcatc atcaaaacaa actgaaccac gttgatgaga aagtagggtg tgattttgcc
     9361 cagcgtcacc agattcgcgg acacgggata cgtgaaaagg cgctggagcg tcccgtcttt
     9421 cttttccctc aggaaaccga cggacatggg aatggcgata aaaaacatcg cgaaaatggc
     9481 gtaggccgga accgtctgct gcagaggcgt gggcagctcc cggttggggc cgcttcgctc
     9541 gacgaccaga ccggacacaa ccgcttccag attgtcgacg cccatgacca cttccacggc
     9601 aagcgacgtg atgagcgagc gggcggcaat ccggtagctc gcatccatta cggggtcgaa
     9661 gcgaatttcc accggccggt ctccttcttc gaaaccgggg ggaatcgtca cgacggcctg
     9721 cgctgtgcct tcggcaaaaa ttcgatcaac atccgctccc gccggccttt ccaccggcac
     9781 gatcagcgca tgcgccttga tttttcccgc aagaaggttg gcctggtaag accggctctg
     9841 gttgtccacc accatcgaaa tctttcgccc gaccagtttg tccgcccaga cgccttccag
     9901 ggccagcgac atgaagaaaa tcagagccac cggcatcagg aaaagaagca ggatggtctg
     9961 ccggtcgcga acgaggacca gcatttcctt gcggaccagt tctattagtt gtttaaagat
    10021 cacgaagccc cctgcccgtc ttcttcagaa aaaattcttc caggttgccg atatcggaca
    10081 ttcggccgtc ataaatccgt tcgccccggt ccagaagcgt caggccggag cacaggcgct
    10141 gggcctcttc catcatgtgc gtgcaaagca gaatcgtgac gccacccgcg tttagctcca
    10201 tgagcaggtc gtacatatgg tttcggcttt gcggatcgac gcccgccgat ggttcatcca
    10261 acagcagaag cttcggggaa tgaagaagcg cggccgccag gttgagtcgc ctttgcatgc
    10321 cgccggaaaa agacgccacg gccgcttcgg aagactccgc gaggcccgtt ttttcgagcg
    10381 cccaatccga gcgcgaagcg atctgatcct ttgacattcc cgccatcgcg ccgaaaaagc
    10441 gcagattctc ccgcgcggtc aaagacgggt aaagagatat cggctgcggc gcgcacccga
    10501 tgaacgaaag cgcttttgag tcgggcatgc gaaggccgta cacggacacg ccgccggttt
    10561 ggccctgtaa ctggccggtg atgatccgca tcagcgtgga cttcccggcg ccgtttggcc
    10621 ccagaagcgc gtgcgtccgc ccgtccgcga cggccatgga aaaatcccga agcgccggag
    10681 cgggcgatcc gtcatagcaa aaggtcacag aagaaatgtc gagcgcgttc atgccttgag
    10741 cttccctgcc gccttagcca gatccttgaa gagctgttct tcgcgcccgg cgcagatcag
    10801 catcaggccc cccgcttttt caaacgtctc ttccgtctgt ccgcgctttt taatgatgcg
    10861 cgcggagacg acggcaaatt ccctccagat gtcgccgatg gccgtcattt cgccggataa
    10921 acgggcaagc tccttcgatg cgaaaagctc ggcggcttcc tgaagaaacg ccgcgtacat
    10981 gtagcggaac cctgcgccgc ccgtgcccac ttcctcctgc attcggacca cgctggacaa
    11041 ctcgcggcag gcccccatga agcccagctt ctcggggctg cgaatgattt ttttagccag
    11101 atagcgcatt ccccgaatgc cgaaaatggg cagggggatt ttcagcatca ttttgcaggt
    11161 gtctttcatg ccctgaaggc cggccgcgcg caggtcgggg ttggggttga cggattgtgg
    11221 atagtacatg aaccctctcg attcgagcgg accacgcgca aacctcgccc tttccaggtc
    11281 ttccgccgga caatccaccg gctcctccag catcgggtcg ctcacgcgaa aaccgttctc
    11341 gatctcatgc agaacgacga tattatgccc gttgaactga aagcggaaac ggttgggaaa
    11401 ataggaaagc cagtagatat tggtggtcag cccgacgatc tggccggtgc cgaggacgcg
    11461 cctcagttcg tccattccct tcttcgggtt gcgaaaccgc gcggttttga atttgccgcc
    11521 gaggcgcttg gcggatttac ggaaaacggc ccccggctgg gagcgaaacg tgctgatcgt
    11581 cacgccgccc attttcacaa acggcagatg gccgaaaaag atgccgctcc cgatgccgaa
    11641 aatcatcggc tcggagattt ccacgccttt gtcccgaaac agcgcggcgg tgacgccgct
    11701 ttcacagtgc gcggaatgaa cgtggataaa gggtttcccg gctgcggtga cttccgtggt
    11761 catgcaggct gcctctccag aatcaggttt gcggaacggt tttgagctca tctagggtaa
    11821 cggccagcgc cctggcgtat cggtccagaa ccgacggacc cagtttggaa aacacgtcgg
    11881 gacggaaatg ccgtctgacc cgaaaagccg cgacgcgcgc cgtttttccc agaatctccg
    11941 gcgtcatctg gcgaagctcc atgtaatagc aaagcggact tttgacgccc gccagcaccg
    12001 cctgcctcgt cgcctccaga cttgccgcca tttcttcaac ggccaggccg ttgaccaccg
    12061 cttccacctc ccagccggag ctgggcacaa tgacatattt cccctcgtca ttgagcgccc
    12121 agcaggctcg cttttttccc tcgtaataag tgatgtcctg cggcacttcc cgaacgttca
    12181 tttcacgtcc tcttttaaag atttcaatgc ctttctatac taatttgccg cgtgggcaaa
    12241 gcaaattctg cccggcggcg ctcctctacg ggatgatctt ttcaccgaga aacggctcga
    12301 aatgatgcca ttcaaaagga tgagcggcgg cgaagcgcgc cagatcatcc gcataggcct
    12361 gcacggattc ctgcaccgct ttttttcttt cggcgcgggc ggccgccgcc accttccggc
    12421 cttttgaaac ctgcacgtgg tagtggcccg gcgctttttc atggacaaag aacgtcatca
    12481 gcggcgcacc gctcatcagc gcaaaaagat gcggcgtatc cggcagacgg actttgtgct
    12541 ccagaaaatc aacttccacc gaggtctggt cgccccagag cctgtcgccc gccatcgaga
    12601 cgatgccgcc ttcacgaagg aagccgacac cctccagaag cgcaaaaggc gacttttcct
    12661 tttcgtccgt ggtgacgatg cgaattccgg tttgggcgag tttttccttt tgcatctttt
    12721 cgatctgctc cttgtgcttg gccccaaggt aaagcatgac cggcaggccc ttttcctgca
    12781 acctctgagc ggcaagctcc cagtttccga tatgcgacat caccacgatc gcaccggttt
    12841 ttttggctac ggcctcatcc agatgctccc agccttctcg ggtgaacttc gcctcctcgg
    12901 ccgcccaggg aatgaagcgg tgaacataga cgcccgtaaa gttgtggaac tgcttccaga
    12961 cgcagtagag gtgatatcca aagcttcgtc ccgggaaaag cgcccggtaa aactgaaggc
    13021 tgtcccggac gcggggcgga aacaagaaaa agtaccccgt cgcgataaac caggaaaaca
    13081 cacggaaaaa ccacagcccc agacggcggg acagataaat cgaaatttta taaaataatt
    13141 tcttcatcgg atttccaaat cgtaaagcaa cgtcattgtt ttgccacagg cgccctttaa
    13201 atgcaacccg tcgttttggc tgggtttgcg aatgcgttgt aaatatttca aattattgaa
    13261 tatatttcaa taacaaaaac acctgttgac tatcctttgc aattggtata ttggttcaac
    13321 catcagacca attatacggg ctggtgcagg aaaggaaaaa gttaatgacg gcaaaaaagg
    13381 ttttggcgca gccggtttcc cgtgcgcgcc tgaatgaaga gattgtcacc gttttacaaa
    13441 atcagatttt gtcgggcaag cttgctccgg gagacaaaat accgccggag cgggagctgg
    13501 cggaaacctt tcaggtgaac cgcgccacgc tgcgcgaggc cctgcggaag ctggagattc
    13561 tggaacttgt ggaaatccgg cacggcaacg gcctgtatgt caaaaattat ctggacagcg
    13621 gcaatctgga tctgatcaga gccgccgcca atctggacgg gcgcgaagaa gtggtgctgg
    13681 atattctcga agcgcggcgt tacctgatgc cgcagatagc ctggctcgcc gccgcaaggc
    13741 gttcgaatga ggatcttctt gaactggagc gggtgaccgg gagcgaggag ctgaccatgc
    13801 tcgaacggga tatcaaagtg caccagatca tcgcccgcag cacgcataat ctgctttgca
    13861 ccatcgggct gaatttcttc aaccagatct ttcgcgatta cggacctctc tattttaacg
    13921 atgaaaaaaa cgcacagcgt tcccggaagt ttcaccggga tatttatgaa gccatcaaaa
    13981 gccaaaagcc gatagaagcg cgcaaaatca tgagagaaat aatgatttac gccgaagagg
    14041 tcgtcaaagc gggcttgtcc ggaaacaaaa agcagaagga atcctgaaaa tgttaagatt
    14101 tatagagaat tacacaaacg aaaaatttga cgtgctggtc gtgggcggag gaattaccgg
    14161 cgcatcggtt gcttatgaag ccgccgcgcg cggattgaaa gtggcgcttt tggaaaagaa
    14221 agatttttcc tgggccacgt catcggtcac ctccaaattg atccatggcg gtttgcggta
    14281 tctggccaac ggcgaaatcg gtctggtgcg cgaatccttg cgcgaacgcc gcgtgctgga
    14341 aaacatcgcc cccaattttg tctatccgat ccccatcctg atgacgcatt ataaaaagcc
    14401 cctcaaaaac agcaaatggg tggtaaaagc cggcatgctg atgtatgacg cgctttccta
    14461 cgacaaaaaa tatacctggg atgccagcaa aaaaatcccg cggcacaaga cgatttccag
    14521 acggacggtt ctggaaagtg aaaaaaacgt gcgccccgcc ggtttgacgg gggcatcgct
    14581 gttttatgac tgcatgagca tttttccgga gcggctgacc ctggccttta tcaagtcggc
    14641 ggcggcctgc ggggccaagg tggccaatta tgcaaaggtg gaaagctttt taaccgatgc
    14701 gcaaaaaaac gtcgccggcg tgcgggtgaa agacctttta accggcaaaa ccatggccat
    14761 ttcctcatcc gtaacgatca actgcgccgg tccctgggct gatcttctgc tgggtctggc
    14821 cgaatcgaat tcggccggcg gcgcggcgct gcggcgttcc gagggaattc acatcatcac
    14881 gaaaaagaag ctggtttccg gcaaatacgt ggtggcctgc atgacgccca aaggccgcca
    14941 ttttttcctc attccctggc gcggccatac gatcatcggc acaacggata agccgtttgt
    15001 cggcaatccg gacgcctacc atgtcaccaa ggccggcatt ctggaattga ttgacgaagt
    15061 caacgggtca ttcggcgacg gaacgctttc ttacgcggat gttgtgcaca cctatggcgg
    15121 tctgcgcccg ctggtggaag gacaaaccga agaaacctat accagttcaa ggcgttatga
    15181 gatattggac aataaggaaa acggactcaa cggactgatt accgctgagg gcggaaaatt
    15241 caccaccagc cgcaacctgg ccgaaaaatg cgtggatctt gtcgaggcca aactgggcag
    15301 gcccagggcg aaaagcaaga cagcccggca tcatcttgcg ggctgccgga tcaaaaattt
    15361 gaatttcttc atggaagaaa ttcaccagca caacacaaac ggcttttgcc gaaacacact
    15421 ggattacctg gggcgctgct acggcacgga ctaccgggca attaccaaac tagcccatga
    15481 aaacaaggag ctggctgaac aaatcaacga agacggcgag atttttgccg aggtggttta
    15541 tgccgtgcgc gaggaaatgg cgcgcactct ttccgatata gtgctcaggc gcacgggaat
    15601 cgccacgctg ggcaatcccg gcgagaagat tttgcgcaag gtggcggtgg tggcggccaa
    15661 agaactgcaa tggacgccgg aaagaatgga acgggaaatt gccgggacca tcaacacgct
    15721 ccggctgccg caataacaag accggattac agggaggaca gccggatgac agccacgaat
    15781 ttccgaccgg actggacgga aaaagcaccg cccgcgggaa cctaccgatc gatctttaaa
    15841 tatggaaatc caaaccactt caagcacccc agcgatgcct ggtatgcaat gctcaagcag
    15901 gattttcatc tcaccgacga ggatttttcc gccaaatccg gcgaaggcct caatctggtt
    15961 tcactcaacc ggccgtccgc gctgaagccg ggccaaatcg aagcattgcg cgccatcgcg
    16021 ggcgtggaaa acgtcgcctg cgacgattac agccgcttaa aatattcctc gggaaaaacc
    16081 acggaagaga tgctggagct tcgggaaggc attgtccggg aggtggcgga tgtggtggtg
    16141 catccgcgcg acaagcacga tgtgcgcaaa attgtcgaat actgcgatcg ggagctgatt
    16201 ccggtgacgg ttttcagcgg cggctcctcc gtcaatttcg gctgccgccc gtcccggggc
    16261 ggcgtgtcgc tggcgatgag cacgcacatg aacaaactac ttgaaatcaa cgaaataaac
    16321 cagacagcgc gtatgcagcc gggcatgttc ggaccggcct gcgaggacgc ccttaaccgc
    16381 gcgcctcaac tctggcagac gaagcatcgc tacacctgcg gccattttcc gcaatccttt
    16441 gaatattcga cagtgggcgg ctgggtggcg acactgggct ccggacaggc ttccacttac
    16501 tacggcgacg cctgcgacat tgtgttcagc caggaatacg tcactccggc cggcacattc
    16561 aaaacgctgg attatccggc aacggccacc ggccccaagg taaacgacat tatgaagggt
    16621 tccgagggcg ctttcggcat tctggtggaa gtgaccatga aaattttccg ttacctgccg
    16681 gaaaacagaa cgtacttcag ttttatgtac ccttcatggg aaaaagccgt cgaagccagc
    16741 cgcgaaatca tgcagtcgga attcggccgt ccggccgtct atcgcatttc cgatcccgaa
    16801 gaaaccgacc ggggattgaa gctctacggc attccggatc tggcggatcg gtttctgacc
    16861 ctccggggtt ttaagccgct ttgccgctgc ctttgcctgg gcaactgcga aggagacaaa
    16921 gattattcgc gcctggtggc aaaaaagatc aagtcgacag ccaggcgcca cggcgccgta
    16981 tcgcttagcg gctacgccgc gcgcaaatgg gagcagagcc gctacaccga accttacatg
    17041 cgcgaagatc tgggcgatta cggcattctg atcgacacgc tggaggcggc ggtaacctgg
    17101 gacaatcttc accgcctgca tcagggagtg cgcgcctata tcaagagccg gccggacacc
    17161 atgtgcatga cgcacgcctc gcacttttac cctcagggaa ccaatcttta tttcatcttc
    17221 atcatccgga tgaacgatcc cggggaattc cggaaattcc agcggggcat tatcgacagc
    17281 attcagaaat acggaggatc cttgagccat catcacggca tcgggcgcat gatggctccc
    17341 tggatggaaa cgcatctggg aaaagagcag atggcggttc tgcgggcgct gaagaatcat
    17401 tttgacccgc gcggcattat gaatccgggc ggacttttgg aattgaaatc atcaggggaa
    17461 aaacgatgga aaacgtgaag aaaccaagcg gagaaatgat tttgtcgttt gacgcgggaa
    17521 cccagtccat tcgggcggcc ctgattgacg cttccggaaa tatcgcgcag atcgtcaaac
    17581 gccggattga accgtatttt tcgcctgagc cgggctgggc ggagcagcat gcggattatt
    17641 actggaaaat gttttgcgag gcctgccggg agctgctgga caagtcggac aaatcatcca
    17701 gagaggcgat tgtcgccgtg accctgacca cgcagcgcgc caccatgatc aatctgggag
    17761 aagacggacg gcctctgcgg cccgcctgtc tgtggcttga ccagagaaaa gcaaacgccg
    17821 aagagatcgt tccctccttt gccaaaccgc ttctgaaatc gataggctta tgggaccgtc
    17881 tcaataatgc ggtccagagc tgtccgtcaa actggataca gcagcatcag ccggaaatct
    17941 ggcggcaaac ccataaatac cttcttctgt ccggattttt cacctttatg ctgacggggg
    18001 aatatgcgga ttcggcggcc tgcaatgtcg gctatcttcc cttcgacaac aaaacgtatc
    18061 aatgggcggg cagattcgat ttgaaatggc ggctttttcc catcgaaaag gagaagctgc
    18121 cccgcctggt caaaccctgc gaggttctgg ggagaatcac cgcccgggcc ggagaggaaa
    18181 ccggaattcc cgcggggctg cccgtcattg ccgcaggaac ggacaaggga tgcgagatgc
    18241 tgggcgcggg ctgtctgacg ccggacaccg gctgtttgag ttttggaacg accgccactt
    18301 tcaatacgac gacgacgaaa tacatcgaac tgctgtccat gattccgccc taccccgcct
    18361 ccgtgccgga tacctattac acggaagtga tgatctatcg cggtttctgg atggtgagct
    18421 ggtttcgcga tgaattcgga atgctggagc ggaagatagc cgaaaaaaac aatatcgctc
    18481 cggaaaagct ttttgatcag ttgattgaaa aagttccgcc cggttcgatg ggcctgattc
    18541 ttcagcccta ctggtcgccg ggaaccaata cggatcccta tgccaaagga tcgattatcg
    18601 gtttcgggga tgtgcacaac cgggcttatc tgtaccgggc cattctcgag gggctggtgt
    18661 acggactgag agaaggcgcg gaactcactc aaaagcgaac gggaacggcc gtttcccgcc
    18721 ttcgggtttc cggcggagga tcgcaaagcg atatggccat gcgcattgcg gcggatatct
    18781 tcggcctgcc cgcggagcgc cctcatacct atgaaacttc ggccctgggc gccgccattg
    18841 acgcggctgt gggacttggg ctttatccgg atttcccgtc ggcggtcaag gccatgacgc
    18901 gggtaaagga agtatttgag cccgttgccg aaaatcaccg gatctacgac cgtctttacc
    18961 gggaggtgta tctcgccatg tacggccgct taaggccgct catggaaaaa attcaagata
    19021 ttacgggcta tccttcgcct tcgaggcaat agccttcacg ggctgcaaat ctgttttttc
    19081 gaccggttga actatttacc gttaatcgtt tccatgattt ctttaaaact tgcaactgcc
    19141 gactcaaggt tctctatgat atcgttggcc agcgcatccg ggtggggcag attgttgaga
    19201 tcggcaaggc ttttgtcttt tagccaggca atgtccaggc tggttttatc gcgggcgaca
    19261 atttcatcat aggtaaattt gcgccggcgg ccttctgggt tttttccgga tgccacgttt
    19321 ccttgcgttt atggcggttg gacggcttgt agcattcaat aaaatcctga aggtcatcat
    19381 acttcacagg attgtatgca gatcggttgt ttccagcaat tttttgcgaa cgatttcacc
    19441 cgcgccgccc tcgaacaaaa cattatccgg cacaacaacc gaagctttgc ctgtggtttt
    19501 cagcattgtg cgaatgtgct ggacaaaatt gagttgcttg ttgctggttg tcgcccagaa
    19561 atcctgccgg ttgtaagtaa gatcgtcttt ctcctgttcg ccttcttcat tggtgaaggt
    19621 catggaactt ttcttcccga agggcggatt ggtcagcaca taatccacac gcaaacccgt
    19681 atcggcaacc agtgaatcag taggcaggat gaacgtctcg gcatcaatat cgccgatatt
    19741 gtgcagaaac atgttcatga gcgccagccg gcgcgctgcc gcgacgatct cgttgccgta
    19801 atacgttgaa ttctttaaga ataatttttg ttctttgttc aggataaagt tttgcggatc
    19861 ggaaatgcaa tcataaaccg ccagaaaaaa gccgccggtc ccgcaggccg gatcggcaat
    19921 ggttttcagg ggctgcggtt gcgcattttg ctcagtcaac gattcggatc ctgagcttgt
    19981 cgaagcatcc aataagttca taggctcgcc agacattcaa cggtgcgagc gtggatttat
    20041 cggctacaat agcatctacc agtttccgat gtgcgacatc accacgatcg caccggtttt
    20101 tttggctacg gcctcatcca gatgctccca gccttctcgg gtgaacttcg cctcctcggc
    20161 cgcccaggga atgaagcggt gaacatagac gcccgtaaag ttgtggaact gcttccagac
    20221 gcagtagagg tgatatccaa agcttcgtcc cgggaaaagc gcccggtaaa actgaaggct
    20281 gtcccggacg cggggcggaa acaagaaaaa gtaccccgtc gcgataaacc aggaaaacac
    20341 acggaaaaac cacagcccca gacggcggga cagataaatc gaaattttat aaaataattt
    20401 cttcatcgaa ttcccgaatc gcagaccaaa tgtaattctc tttgccgcaa tcttccttta
    20461 catgcaattt ccctgattta gcaacctata aaacattcca cggaacaaag cattttcggc
    20521 catatttcaa tggaaccgca atatagccga atggaacggt tgtcccacca ggttgcattt
    20581 tcattggttt tgtgctacca aagaatcatt caaaaaagac acttgtcatt ttttgatcac
    20641 ccagaggctc ccgtcgttct cataacggca caacataaac aatcaatgtt caggagatgg
    20701 acttgaaaaa agatccaacg gatatgtatt ttcgccggcc ggaagttgct gatgcgccgt
    20761 tcattgaaaa gcttgttaaa gattctccgc cgctggatct caattcgctt tattgttatc
    20821 ttatcctgtg cgcgcatttt tcccggactt ccgtcgtcgc ctgcgatgca gacgccgttt
    20881 gcggctttat ttccgcctac ctccgtcccg atgaggagga cactttgttt gtctggcagg
    20941 tcgcggtgga taaaaagtat cgggggaaag gcgtcgcccg ctccatgctg tcttccattt
    21001 tgaaccggcc caccccctgt cccccgcgtt acctggaaac gacggtcagt ccgtccaata
    21061 ttccgtcaaa ggcccttttt tcttcactgg ccgcttcgct tggcgtcccg ctggtgcaaa
    21121 gcgttctgtt ttccgccgat gatttcggag atcagcgtca tgaagaggaa gtcttgttcc
    21181 gaatcgggcc gtttgacctg tgatcaaagg aggaaatctg atgaaaacca ttagacgttt
    21241 ggagtctgaa gtccgcagct attcccggtc gttccccgtc ttgtttgaaa aggcaaaaga
    21301 cgctttttta ttcgacgcgg acggcaaccg ctacattgac ttttttgcgg gagcgggcac
    21361 gctcaattac gggcacaaca atgaccacat gaaaaagaag ctcatcgagt atttggaaac
    21421 ggacgggatc gttcatggtc tggacatggc caccgttgcc aaggaagagc tgcttcttaa
    21481 aatggagagc attgtgttca aaccgcgcaa cctcgattac aaaatccaat tcaccggtcc
    21541 caccggcacc aacgccgtgg aagcggggct taaactcgcg cggatggtga aaaaacggtc
    21601 aaacgtgatt tcatttacca acggctatca cggtctcacg atgggatcga tgtcggtcac
    21661 ggccaatgcc tactaccgcg acgaggcctt catcaaccgt tcggacgttt cctttctgcc
    21721 ctacgacggc tatctgggaa aagacgtcaa tacggcccag tatatgcgac gctttcttga
    21781 agataacagc agcggcgtcg atattccgtc ggccgtcatt cttgaaaccg ttcagggcga
    21841 gggcggaatc cgcgtggcat ccgacgaatg gctgcgcgaa gttgaatcca tctgccgcga
    21901 gttcgacatc ctgctcatgg ttgatgacat tcaggtcggc aacggccgca ccggacattt
    21961 tttcagtttt gaacgctccg gcatcaaacc ggatatcgtc acggtttcca aatccttttc
    22021 cggctacggc ctgccgatgt cgctggtttt gttccgccgg gaacttgaca tctggaaagc
    22081 cggtcagcat accggaacct tccgcggaaa caatctcgcc tttgtttccg cttccgtggc
    22141 gctggactac tggaaggacg accgtttctc caaagatatt ttccgcaagg ggacaatcct
    22201 ttccgaccgc ctccatgcca tctgcgccag atatcctcag ttgaacgcgg aagtacgggg
    22261 gcgaggattc atttacggga tggccgtcga cccgcccgaa atcgccagag aaatttccat
    22321 ggaatgcttc aagaacaaac tgatcatgga attggccggc gccagaaaca atgtcatcaa
    22381 attccttgcc ccgctgatta ttcccgatga ggtgctcatg gagggcctgg atattctgga
    22441 tgcgtccatc ggcagcgtgt tgaaaaaccg ttaaaggaaa ggacaaggca tgctggttcg
    22501 aacgatcaat gatatcacgg gaacagatcg cgaggtctgg gctgaaaata aaaactgggt
    22561 cagccgccgg cttctgttga aaaaagacgg aatgggtttt tcgtttcatg aaaccatcat
    22621 cttcgcggga acacaaacgc acatctggta caagaatcat ctggaagccg ttttctgcgt
    22681 tgaaggcgaa ggcgaggtgg aaacgccggc cgacggaaaa attcaccaca tcgtgccggg
    22741 aacaatatac gcgctgaaca accacgacga gcatcttttg cgggcgtcca aagacatgcg
    22801 tctgctgtgc gttttcaacc cgcccgtcac cggcgcggaa gtgcatcttg aagacggatc
    22861 ttatccgccg gcggaaaatc aagggaaagt tccatcttga tggagcggca caaaacaaat
    22921 ggaaccattc ccttgccttc ccggggttga aggattgttc cgttggaaag ggaaagaatg
    22981 aaaatttgcc ctccattcat ccggcaaatt tgccttcctt tttcagcttt tcgacttcct
    23041 ccttctccag cgtgttgaag gcgattttgc cgaccagggt tttgggcaga tcagtgcgga
    23101 attcgatgga acgcggacag ctccacttga tcagattctc gcggcaatag tggatgagtt
    23161 ccttttccgc tgccggaccc gcgtcggcgg ggtttttcaa tacgacgaac gccttcaccg
    23221 cctgcacctg ggcttcgtcg ggcacgccga tgacgcaggc gtcgcgcacc ttgtcatgct
    23281 tgtagagcag ctcttccacc tgcgcgggat agacgttcat tccggaggac ttgatcatcc
    23341 gtttctgacg gagcttgaaa tagaagaatc cgtcggcgtc catcgtgccg atgtcgccgg
    23401 tgtgcagcca gatgcggccg tccgcgtgct tccggagcgt ggcggcggtt tcttcgggct
    23461 gatcgagata gcccatcatc acggcggggc cggctacgca gatttcgcct tcttcgccca
    23521 gcggcgcctc ctcgatcgtg tcgagcttca cgattttggc cagcatgtcc ggaaaaggca
    23581 caccgatgct gccttcgcgg tagcagccga tgggcgtggc catgatggcg gtgacggcct
    23641 cggtaagtcc gtagccttcc agaagctgaa cgttgccgcc ctgcttcctg accatttctt
    23701 caaagcgctc cttgaccgga cggggcagcg tgtccgcgcc ggaaaatgtc gcccgaaggc
    23761 agctcaaatc cgccttgtgc attttgggat tgcgcgaaag cgcatcaaag agcgtcggca
    23821 caccaatgac aaacgacggc tttcgcgaac ggatcagatc ggcgaccgtc tcgggggtaa
    23881 agaccggcac cagaatgctt ttgccgccgc ccatgaagca ggcgttgatg cagaccccca
    23941 ggccgaagcc gtgaaagatc ggcaaaatgg ccagcacgga cgcgttgtcg tcgagcttcc
    24001 cccattccga caccattttc ccctcggaaa tgaaattcat gttggacagc atgatgccct
    24061 tgggaacgcc cgtggttccg ccgctgtaga ggatcacggc catatcgtcc gtatccattc
    24121 tggccggcgg ggcgctggga taaccgcctt tcatcagatc cgcccaccat ttcaccatgg
    24181 ggtccgccgg gacttttgga atctgccgtc ctttggtcag gttgaagccg acgcgtttga
    24241 aaaagctcag ataatccgga atgcgcgcca gaatcagcgt ctgaagcggc gtcgcggcct
    24301 tcacctcctt gaacttggcg taaaaggcgt ccagcgtgag cgcgaagcgg cttttggcga
    24361 cgttgaggta gaattcaatt tccttggccg tggaaagagg atgaatcatg ctggccaccg
    24421 cccccagctt gttggccgcg taaaaagcga tgatgccctg aggagaggtg ggcatggaga
    24481 tggtgatgcg gtcgccctgc ttcaggccca gcgcggccag cgcgtcggcg caccggtcga
    24541 tttcggaaat gaaccccctg taagtggacg cgtgccccat gaaatcgtag gcgactgcgt
    24601 cggctttctc ccgcgcggtt tgcaaaagcg cttcatacat ggtgacgcgg ggataatcga
    24661 tggaatgcgg aacgttgccg taaaacttca accatggttt ttgctcgtac attgtgttgc
    24721 acctcccgaa atagttgatg gtgacggatg aatcttggat ggtcaaaggc gacctgacag
    24781 gactctttcc cctcctgttc aacacagtct aatagccttt tccccctaca aaccactttt
    24841 cttccacgcc gggaaccggc gaggcatgca ccctgacggt aagtcccgcc gcctccataa
    24901 atccggcgat ttctctttct ccgcggaagc gctccgggat gcccgtccac ttcaagcggg
    24961 ccgtctcgat ccatcgcttc caggggactt ttcgatcgga gggaaccgtc gcccggatca
    25021 ccagcatccc gtcgtgtccg agcttgtcgt gaatgcgctg aaaaacaagc tgcaattccc
    25081 tatcgtcgat cagatgcagc atatccagca tcagaacgtg gtccaccgaa ccttcgacgt
    25141 ccggcagatc cggcgcccgc ccgacctgca cgtcgccccg cgcgccgatc acgcggcggg
    25201 caatgagcgc cctctcttca tcgggctcca ggccgtaaac cttcgcttcc ggatgcagct
    25261 ccagcagcca ggccgcggga acgccgtagc cgcagccgat atcgagcatc cggcggggat
    25321 tttgcacata tcgatgcagt tcggaaaaca tcggatccag cttcatcttg aaccgggcaa
    25381 aaaccctcgg ataaccggga agcagccggt agcgcaaaag gacgcggcga acatgctccc
    25441 gcgatgccgg ctggaccgcg cccgcgggaa agtcaaacgg cgaaaaaatt ttccgcatca
    25501 gggtcggcaa aatgaaatac gcgcccagca gggaataccc gatgcccaga agcgaaacca
    25561 ggccgatgct gcgcaggagg gagtgattcg ccagcgtcag aacgccgaaa ccgataaacg
    25621 tcgtagcggc ggaaagaaag atggagagct tgacggtgtt catcgcggga gaggcctcct
    25681 ccggatgacg ctgataggtg caggtgtagt atatggcgta atccacgccc atgcccagaa
    25741 tgacgaccca cagcatgatg ccgggaatgt ccagcggacg gccgatgagc ttgagcgttc
    25801 ccagcgtgga gatgagcgca aaagccacgg gggccagaac ggccagcgac aggcgccagt
    25861 ccagaaaaaa caaaaatacc accgcgatca ggccgaggct gacgatcagg gcaatttcga
    25921 taaaaatgct cttgagaaat tcccccatgc ggcgattgaa gaaatccgcg tcgaacagat
    25981 cgatctcgcc ggctgcggcc agacggtcaa aaaattccga agcgttgtaa tgcttcccgg
    26041 cagccatcgg cgtgagctgc gcgaagcctt cggacgtccg ggcaatgccc agcatttcca
    26101 gatgtttcgg tggaatcgta aagacgccgg gccgctgtgc ctgaaagact gtccagaagg
    26161 gatcgaacgc gtccggcgca aagccgtttt ccgcggccgc cttttccaga tttgtcctga
    26221 gcgccccgac gcgctccggc gtccagaagg agcgccaggc ggcaaaatga cgggcggccg
    26281 actccggcgt gggaaacaga acggtcggca gaaacgccgg ggccagtctg ccgcctttca
    26341 cttccctggc catcagcgct tcgagctgat cgtttttctt ccgcagccgc tccaggtccg
    26401 gcgcctccag aaggacatac aggcggccgc tgagatttcc ccaggtgtcc tgcaatttct
    26461 tttcggcggc aagcgttgcg gagctgacgg aattcatggc ctgcagatcc acgttgaaga
    26521 cgggtttggc gaaaagcagc atcgtcagtc cgaagaccag cgcggcgatc agtttccatg
    26581 aagccggcgc ggctattttt tggacggcgc ccgtcagcag gcggttggat gcgcgacggg
    26641 cgggcggcat ctccggaaag ataatgggaa aaacgaagtg aacaaacaga aaagcaaagg
    26701 tgatgcccag cgcggcaaaa acgccgattt gggccagaat gtcaaaatcg ctgatcagca
    26761 gcagcaaaaa ggcgcagatc gtggtcaggg cggccgtgaa ttcggccgac cagacttccc
    26821 gcgccgcctc gcgcccgaac gtggcacgcg gctgatccag aaacaacaga taggtgaggc
    26881 ccagatcgac ggtaaaagcc atgatcgcgc cgccgaagcc caccgccagc atcgacatcg
    26941 acttgaataa aaaagagcag gcaaacaggg cggcaatggc cccgacggct gaaggaagca
    27001 gcgccaggag gccgatgagc gggcggggaa aagacaaaat cagcagaagc gcaatgccca
    27061 gcgtagtgag caaaaccgcc agccgcgtgt cgcgcctggc gacggtttcg ttgtccagcg
    27121 ccgcgcggta agcgcccacc ggcgtcagcg tgaatcggcc gttcgaatcc cggcgcgcct
    27181 ccagcatcct cgcgcaatcg tccatcagcc cggagatttg cgccgcccgg gttgtatccg
    27241 ttccggagcc ggccagccgc gcgatgatga gcgcgtggcg gccgtcggaa gacagaagcc
    27301 ggccggaata aaactgggcg gaaccggccg gcgagagcga ggacattcgc tccagaatca
    27361 ccggggaaaa tcccagcgga tcccgggaga tcatttccgc acggccgatg ccctgaagct
    27421 caccgaggct gatccggttt tccgccatcg tctcacggat tttttccggc gcgagaagcg
    27481 gcgcgatttt ccgctcgaga tcctccgtgc cgaacagcga cggcagatgc tcccggacat
    27541 gcgccatcag ctccggaaac gcacccgcct catcggcgat cccgactcgt tcaaacaagc
    27601 cgcttttgct taaccggtct tcaacgagcc ttgccgcctc gaccagttgg tcgcggtcgg
    27661 aggaggtctg ttcaagatcg atgaaaagcc ggtcggaaac gggcagatga gtgatgaccc
    27721 gccgggcgga ggccagcacc gggtcatggt gcggcatgga ttcgagaatg tcggtttcaa
    27781 tttttaaacg gctggtttcc cagcagaaca gaatccccac gatcagagcg gtgaccagaa
    27841 ccatcgacca tcttatggga tatcggcgag gcggcatgaa gaccttcaga ttgtcgggat
    27901 tattttcctg ctttctttgc tgtttttaaa ccacatttcc caaaggctgg caagaaaaaa
    27961 ccgggcatgg aatcagggat tggcctgtgc ggtcaatgac ggccgaaaac acgggtaaac
    28021 atcaggcggg taaaggtttt ggcattgcga aggaaatcga cgaatggacg aaaatgagag
    28081 attcgcgccc catccggcag gtaaaggacc cgaatcggcg cttcgatgat cggaatgccg
    28141 attcttctgg cctgcaccag aatttccact tcaaactgga accgcctcgc ccggacggga
    28201 aggctcaggg attccggaag cggatagatg cgcatgccgc tctgggtatc cgaaatttta
    28261 ggcccgccgg acattcgaac ccagaaattg gagaatttcc ggccgaagcg gctggtccag
    28321 ggaatgtggg ccccctgcac cgcatccatc ccttcgcgcg ctcccaccac aatgggtctt
    28381 tttttgagag gaatggcatc gatcagatgg attgcgtctt cgggatcgtg ctgaccgtcc
    28441 gcgtcgatcg taatcgcgta atcggctttg tgcgaggcgg ccatgaaacc ggtcaggatg
    28501 gccgcgccct ttccttcgtt tttttcatgg cgaagcactt ctatgccctg aacttccgcc
    28561 agccgccggg acgtatcatc ggttgatccg tcgtccacga caaaaacggg aaatcccata
    28621 tcccgggccc ttccggccac ctgtgcaacc gtcgcggcat gattgtaagc gggaatcaca
    28681 aaagcgaacc ggccgagatt ccttcccgcc cgccgcctgc ttttcggatc cttctgttcg
    28741 ttcgctgtcc gttttttatg actcattact tttcactgtt ctcttcctgc gcttttccct
    28801 atcgcccatt gcctatcgcc tgtttttccc atccaccatc cactatccac tatcccctat
    28861 ccaccatcca ctgatttctc ttctattctc aaaaacgcga cccc
//
LOCUS       DAOPAF010000017        28057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1850_length_28057_cov_58.961896, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000017 DAOPAF010000000
VERSION     DAOPAF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 28057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28057
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_28057_cov_58.961896"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLQ77_03225"
     CDS             <1..572
                     /locus_tag="PLQ77_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HPL09722.1"
                     /translation="VSDPVELNSLHTQGSGKIRLEDGKVLTVSYAQNNGRPFRSATQN
                     LLDENRLDRSDASYRGFKAWLKGKSEKEIYEILSHNERYIFFRFVDREPVGSLGQPVT
                     PHRSIATDPAYFPQGAPAFIRLRKPVLDEDYRVVRRVEFSRFVLNQDKGSAIRGPGRV
                     DLFCGFGPEAQATAGSLKEKGELFFLLLK"
     gene            910..1140
                     /locus_tag="PLQ77_03230"
     CDS             910..1140
                     /locus_tag="PLQ77_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HPL09723.1"
                     /translation="MKIKAATAANIGAAIRKKRKEDGLTLADAAALCGVGYRFLSDLE
                     NGKPTAQMGKVLQVLTALGLDLYIASREWPDE"
     gene            1133..2377
                     /locus_tag="PLQ77_03235"
     CDS             1133..2377
                     /locus_tag="PLQ77_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPL09724.1"
                     /translation="MNNHIGNPGLDVYLRDQKVGRLWLDEKRRFVFQYDEAWISKKGA
                     IPLSLHLPLRAETYPDDLSRPFFSNLLPEAEIKRIIAQRLQISVNNDFAMLGSIGGEC
                     AGAVSVLPSGIAPAVKPGYRELKEEELHKIIIDLPRRPLMAGVEGMRLSLAGAQNKLP
                     VYMEDDRIFIASGNAPSTHILKPPIRDLEDTVENEAFCMMLAQKMALPAPPVKIRRGL
                     DQLFIIERYDRSRDKSGQIVRMHQEDFCQALGFLPDQKYESEGGPSLKHCFTLLQEKS
                     IRPAADRMTLLRWVIFNFLIGNADAHAKNLAMIFTDRGPRLAPFYDLICTQVYPDLTE
                     KAAMRIGGENRPSWIQQKHWEKLGEAVAIKPGLIVKTWKDMSSGIIPKAQVMVNDFKK
                     SHGSTGIIEKIMAVIEKRAGEV"
     gene            2564..2851
                     /locus_tag="PLQ77_03240"
     CDS             2564..2851
                     /locus_tag="PLQ77_03240"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPL09725.1"
                     /translation="MNEILLGLAIALQIGDGVTTCFALQKQGVRERNAILRRLFDRLG
                     VVRGLALAKSFGVAVCWIAYAYGGPFAVYTLGFIVALYAVVVINNLKSLCR"
     gene            complement(2899..4122)
                     /locus_tag="PLQ77_03245"
     CDS             complement(2899..4122)
                     /locus_tag="PLQ77_03245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HPL09726.1"
                     /translation="MTEKENQNPAPSGSRDPATILTDHKIKVLLIDDQAIVGAAVKRM
                     LEPETDIVFQFCQDPTQAIPTAASFQPTVILQDLIMPDIDGLTLVKFFRVHPQLKDVP
                     LIVLSSKEEAVTKADAFALGANDYLVKLPDRIELVARIRYHSRGYINLLQRNEAYAAL
                     LASRQALAAELAQAADYVVSLMPPPLSDPTITTAWRFFPSAQLGGDSFGYHWLDENTF
                     AVYLLDVCGHGVGSALLSVSAFNAIRSQTLPDTDFHSPDRVLAALNNAFQMEHHNNLY
                     FTIWYGVFDRRTRELRFASGGHPPAFLISKEGTLTKLMTNNLFIGGLPESAYQGSSVT
                     VPAGANLYVFSDGVYEVDPPEGSMWTLDQLGEYLLQHKMEAGEDIDALYLKMQDYHGQ
                     KILEDDFSMLAVHFT"
     gene            complement(4119..5156)
                     /gene="cheB"
                     /locus_tag="PLQ77_03250"
     CDS             complement(4119..5156)
                     /gene="cheB"
                     /locus_tag="PLQ77_03250"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HPL09727.1"
                     /translation="MTLKIAIAHHDENALKILRRIIAPNAGCAVIWTTGRGSDVIRQC
                     RHGLPDILLIDLELADVNGAKLICDVMKETPCAILVLSNARERQAGKVFEAMGCGALD
                     VIATPVLHSNGFLEGADALLKKIDTMARLLGTASRMEKPAATVHETKKEPRPPLVAIG
                     SSTGGPKALATILAGLPGNFPAAIVICQHVDSQFASGLASWLNSQTPLDVAIAREGMR
                     PRPGAALVAQTNDHLVLGPDGSLHYTEHPRNYPYRPSVNALFESLRQYWPQNDVAILL
                     TGMGRDGGEGLSALFKTGWHTIVQDEKTSVVYGMPAAAVELGGASEILPLDSIAESIA
                     KKITNRKGKPS"
     gene            complement(5153..7528)
                     /locus_tag="PLQ77_03255"
     CDS             complement(5153..7528)
                     /locus_tag="PLQ77_03255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPL09728.1"
                     /translation="MTTDAAPIDPLMLDLFRVELENNTRMLEAGLVTAERDQTPERIE
                     PLMRAAHSIKGAARIVGLNTAVTLAHAMEDVLSAAQSGKRPLTSTAVDRLLKGNDFFA
                     SLSRLDIQELPAALERGTQEITVMAGELRNFLSSPQETTPAPTAAAQAEEKTAGDVPQ
                     KAQDIKADPSPAPRPAEPTPQKPLPAAVLENACPEPQTAPPAVRSRRSQDQGDGGLVR
                     VRSENMNRLMALAGECLVQAQSIKTVYQSLLVLKKEIFEIEKPLREFLERQEANEQDK
                     GAEDLQQTLSGAVSAQQIVSREIVRFERFSRRLEHLAHRLYNEVISSRMVPFSDGLHG
                     FPRLVRDLARETGKQVRFEIAGAATPVDRDILERLEAPLSHLIRNAVDHGMETPEERK
                     AAGKPAEGTLVLEARHVAGMLNISLTDDGRGINPEDLRGKVVEKGYVSQDMAGSLSHA
                     ELFEFLFLPGFSTARKVTEISGRGVGLDVVFNMAHEVGGTVRVDADAGRGARFHLQLP
                     LTLSVLRTLLLSIAGEAYALPLSRIDRVLSLAPADIREIEDRPYCTLDGEHVGILDAR
                     QLFGLPQASTPPAGFSVVIISDRLNRYGLVVDRVLGEENLVVRPLDSRLGKVPNVSAG
                     AILDDGSPVVIFDVDDLVRSIHQLLTTGKLQKIGRGRQALKTGKKRVLVVDDSLTVRE
                     VERRLLENAGYEVTTAIDGVDGWNKLTSHAFELLVSDVDMPRMNGIDLVRTVKADPQH
                     HDMPVMIVSYKDREEDRLRGLEAGANYYLTKGSFHDESLLTAVRDLIGGPE"
     gene            complement(7525..8190)
                     /locus_tag="PLQ77_03260"
     CDS             complement(7525..8190)
                     /locus_tag="PLQ77_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPL09729.1"
                     /translation="MTTNTEKYCWKAKGIYGDGSCPELPRLIHCRRCPVFAAAGRQHL
                     DRNLPPAYLGERAAVMALPKETGRLGTLSVMVFRLCGEKFAVKTIYFQEAAEPSPVHT
                     LPLKVNRIFRGIANINGELLLCLSVADLLDLTFEAQESGPAPAYERMLVVGQEGQRFA
                     FPVEQILGVHRIAPEDLGEVPATLSRSARALTRGIFLLDGSPVGLLDEERLFPAMTRS
                     LNP"
     gene            complement(8187..9524)
                     /locus_tag="PLQ77_03265"
     CDS             complement(8187..9524)
                     /locus_tag="PLQ77_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPL09730.1"
                     /translation="MNAPAAVMEFITHSLGMEQGSAGKDAVRRAVAFAMRQEGVSDAR
                     EYEKLFLSSPAARQKLIDAVVVGETWFFRDRGPFLWLARHARQWLETHPGSTLKILSS
                     PCATGEEPYSIVMTLLAAGLPPSAFTVEGVDASARALETAGRACYPKSAFRGSIGEDV
                     ARFFETTPSGRKVAEAVASLVSFSPENLALPGSLAGRGPYDIIFCRNLLIYMTPEARL
                     RIFDRMNRLLIPGGLLFTGHTETNFWHQRGYQLLPWDRAFALAKPASPAQPAAIRTKA
                     PTKPSVRPGENFPRKTGAAAQEATSSPSNTGQGKTAGKGRGQPAKSETASENFKLSQA
                     DEQIQEARRLADTGDMKGAARLCRECTRKFGPAAEAYCLMGILSMAEQDSNGAEDYFL
                     KALYLDPGHYESLVHISLICRQKGNEKKAALYRERALRTAGNKGGSHGSEKEQ"
     gene            complement(9521..9985)
                     /locus_tag="PLQ77_03270"
     CDS             complement(9521..9985)
                     /locus_tag="PLQ77_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPL09731.1"
                     /translation="MLLLQFQAGTDRYGLDIGQIIEVSSMVSLRAVPHAPPEVAGTFN
                     YRGTLTPVVDLTVLLTGSPSRPRMSTRIILVNYQGEDGRFHILGLLAEAVTEMIECKK
                     ENLQQPVVGVDGAAYLGDTIFDERGSLQVLDMERLLTRDLLFTLFPPEVTNG"
     gene            complement(10000..12198)
                     /locus_tag="PLQ77_03275"
     CDS             complement(10000..12198)
                     /locus_tag="PLQ77_03275"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPL09732.1"
                     /translation="MIKLSLKRKIIGLAVMAAVLPVAVMMILMGSFKTSVSDLASKEL
                     SDVAMANMSQISRDVYGLCETSNDLIQRKINNGLNVARSILKQHGGIETAREKIAWDA
                     VNQLTRETQKIELPKLTAGGVWLGQNRSTSTPTPIVDDVKRLVGITCTIFQRMNENGD
                     MLRVATNVEDLNHRRAIGTYIPNTLPDGSANPVTAAVLKGQSYRGLAYVVNTWYLTAY
                     EPVTDRGGKIIGMLYVGEKLEAVASLRKAIMSMKVGKLGYVGVVGGKNEHRGRYIISK
                     DGKRDGENIWDQKDVHGNLIIQTLITKALAQPKGESFYHEYIWQNPGEPEPRKKISSV
                     IYFEPFDWVIVAGTYEDDFFRPIGHVHHMIKSIFLKMLLAGALIIFLSVLLAAVLARR
                     LTAPLELTTSLAQKIATGDIQQAREDLAGTASTRVIQAGDETGKLLQAFQAMTGNLDS
                     LIGQVHRSGIQVTTSATEIAASARELEATVAQQAASTTEVTATSREISATADELAQTM
                     DGVNESLNETIGMAQEGRSDLSRMETAMRELVKATSSISSKLTVINDRANRISHVVTT
                     INKISDQTNLLSLNAAIEAEKAGEFGRGFSVVAREISRLADQTAIATQDIQQVVSEMQ
                     TSVASGVMEMDKFNDEVRRGVETMAAQSGQLAQIIDQVRIHGPQFTAVKDGMYGQSQG
                     AQQISEAMGQLSLAAEQTKASLHEFKLATEQLNEAIAGLQSEVSRFKISS"
     gene            12380..13264
                     /locus_tag="PLQ77_03280"
     CDS             12380..13264
                     /locus_tag="PLQ77_03280"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HPL09733.1"
                     /translation="MSGKTKKGIAFSNAEREGGRFVHTADLPDREAGGKFHERRCGEM
                     MDEVTIDEILGGRLKIRQSKRGYRFNLDSLMLAGFASIKPRSVNLDLGCGNGILALVL
                     AGRHPLTRWVGLEIQEGLANLARANVEQNGLAGRISIVAGDARNAGKIFSNHSFDQVV
                     FNPPYRKISSGRINPLSEKAIARHELQGALADFLAASKILLKPKGRVFTIYPAARLVE
                     LVSLFRARGIEPKRMKLVFSDASSDAVFVLAEGRSGSGEELKMEPPLYIYEEGKTYTP
                     EMKKLFSDLAAFPEACGG"
     gene            complement(13224..13718)
                     /locus_tag="PLQ77_03285"
     CDS             complement(13224..13718)
                     /locus_tag="PLQ77_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPL09734.1"
                     /translation="MRRRFKGQLQPVGDILSSALKRRGLAGKLADNPVFKLWPQAVGA
                     KIARQTRPDGWRAGTLFVKTTSSVWVQQLHFMKEDIRKKINDLAGKTVVQEIHFGVGY
                     SPPRGDAGRLAVPPGTALKERDKRIIAECTETLKDPELAAVVKRVMQAEISRRRLREK
                     RQDR"
     gene            complement(13715..15703)
                     /locus_tag="PLQ77_03290"
     CDS             complement(13715..15703)
                     /locus_tag="PLQ77_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09735.1"
                     /translation="MNAKPTYEELEQKVALLSRKVNEQQAVENQYRNLVDSTSDSLYL
                     VDADCRYLFINQNHARRLKMPQNDVAGLSYKDIHSPEQNAKFELKIKSVYQTGQSAFD
                     EHQSARDGRYFLRTFSPVRDSSSPEPIIAVAVVSKDITPRREAEEALRKSEEKYRQLI
                     EYSSEAIFIIANGKIGFANSRTEKMLGYPLADLQAMPFLDLVIAEDRDMIDERQQAVL
                     QESEPSLNNRFRMVRRDESTIWVAANSVLMRWENAPATLNFMRDITRQKKAEAKLLQA
                     QKMESIGTLAGGIAHDFNNLLMGIQGHASLALLNLHPGDPNYEHIRTIETLVVSGAGL
                     TRQLLGFARGGKHEVKSVDLNDLIHQTSRTMGRTKKEINIHHHFEDPLWMTEIDPAQI
                     EQVLVNLFINAWQAMPVGGDLHLETKNMIVDENRFKDFSGKGGRYVKISVTDTGVGMD
                     EKTRERIFEPFFTTREMGRGTGLGLASVYGIIKNHNGFINVYSEKGQGSTFNIYLPAS
                     AHQSQNHQCPEEASAPERSSDRETILVVDDEAMVLDVAGEMLRTLNYRVITVPNGAAA
                     IDLYRREQERVDLVILDMVMPDMNGSEVFDAIRRINPGVRVLLSSGYSLNGQAGKIME
                     RGCSGFIQKPFTISDLAGKLRVVLEKPDPAAQCGSSKP"
     gene            complement(15721..16254)
                     /locus_tag="PLQ77_03295"
     CDS             complement(15721..16254)
                     /locus_tag="PLQ77_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPL09736.1"
                     /translation="MQITVNQTVIEIVCADITEEETDAIVNAANSTLSGGAGVDGAIH
                     RAGGPAIMAECRKIGGCPVGEAVITTGGNLKARYVIHTVGPVYRGGGRGEAALLASAY
                     RNSLKLAVKKGVGNISFPAISCGVYGYPVHEAASLALQTCLEFARSSAELKLIRHVLF
                     DERTYDIFAGKLKKLMT"
     gene            complement(16257..17843)
                     /locus_tag="PLQ77_03300"
     CDS             complement(16257..17843)
                     /locus_tag="PLQ77_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPL09737.1"
                     /translation="MSSEKTFPMSRAVLGAYAQGKEDETLLPLVLVDQKQAPLGRIQP
                     GDSIIFYNIRGEREIELTRSFTEENFREFPVHPALPVHFATMIEYSKGLNADVAFPPQ
                     GVLSDTLSDIIAAHGLAQAKITEAEKAVHVSYFFNGKKSDPLPKEERIVVPTRKDVRL
                     FDEAPEMSIDEITRAAGAKIQDPSCRFIVVNFPNVDVVGHIENEPAILRAVSAVDRHA
                     GLLVEQALRRNMTVIVTADHGTVEKWLYPDGGVDTGHTDSPVPFILLHPEQKPALKPE
                     GELTDIAPTILDLLGLPVPDSMTGSSLIQEKPPSLAPASRLLLLILDGWGLCDSQKGN
                     LIACAETPVMDRLLRDYPHARLAASGLAVGLPAGTVGNSEAGHLHIGAGRRIYSDRLM
                     IDQSIARRDFHRNPAFTRAMNLARQNRRALHLMGIVSFFSSHGSIEHLFALMDMAAEQ
                     GVSELYLHSMLGRRGEQAESGARYIRQVEEKCRSLGIGQVVSVIGRYWSMDREENWDR
                     IEKTYRMLVYGEGTPIPAEE"
     gene            18008..18403
                     /locus_tag="PLQ77_03305"
     CDS             18008..18403
                     /locus_tag="PLQ77_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09738.1"
                     /translation="MVQVLLKGREKQQENLYEELLRERAAVLARAGFAVEDALEKLEK
                     LDRRIGEMMNRCQSLQTGSPVGAHPREQQSVFEEIDEIIDQFNATCQKAELQYYYLIV
                     TREALGLRRHETVQKFYRIPSKKKKMRAL"
     gene            18403..19056
                     /locus_tag="PLQ77_03310"
     CDS             18403..19056
                     /locus_tag="PLQ77_03310"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPL09739.1"
                     /translation="MDRYKKQRIRMVEAQIRARAVRDESVLKVMETVPRHLFVDEGLI
                     NQAYSDNPLPIGERQTISQPYIVALMTQALELKGMERVLEIGTGSGYQTAILAKLADR
                     VFSIERVAVLASRARRILDQLNCYNVAIRVGDGSYGWKEEAPFDAIITTAAAPQLPRH
                     LLDQLAVGGRMVVPVGGREAQTLYKITRSSENPSELKREDLGGCRFVSLIGESGWKE"
     gene            19044..19955
                     /locus_tag="PLQ77_03315"
     CDS             19044..19955
                     /locus_tag="PLQ77_03315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL09740.1"
                     /translation="MERMSRHILPRRHFAVFRRRYFGAYLILSMLVLLMPAGCANSQV
                     HRSQASGVYHIVKKGETLYGIARAYSASLQDVAEINNIEDVSNIREGAVIFIPNADQV
                     IEDVLVSSGKAAADEKPSVRKPPPVSAEVLPPPGKIPAKPAKPSRPEVQSVPPGPKSE
                     KTKAPVEEKPQISEEKVVMDKGRFAWPARGSVKTRFGIQPNKTYHNWIKIACPAGTRV
                     KAAAFGTVIFSANLKDFGETVIIRHEKDFATVYTHLKKRSVQADQDVGIGQTIGLAGR
                     ADEAGETYFNFEIRLKGKARNPLFYLP"
     gene            20080..20565
                     /locus_tag="PLQ77_03320"
     CDS             20080..20565
                     /locus_tag="PLQ77_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPL09741.1"
                     /translation="MFKVGDLAVYPAQGVGVIEAIENREVMGKKQPFYVMKIMGNGMK
                     IMIPTNSAKAVGLREVILEKEIPKVYEILRNKDVTIDKQTWNKRYREYLEKIKTGSVF
                     EIASVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQEAKVERDLQMIFTV
                     Q"
     gene            20737..21765
                     /locus_tag="PLQ77_03325"
     CDS             20737..21765
                     /locus_tag="PLQ77_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPL09742.1"
                     /translation="MIRLIVRALLILICSVSGYFIAYHTMAAAGSYAVFQAIFGLIIG
                     LVVAMLVIRIERDIRRQSVRTIAGGVAGMITGLLIALIIGFGLNMIIRIPPNQQIVPW
                     IYLLMIGILGYLGLVIGSKKAEEISFRTTDSRKLMDHRLLDTSVIIDGRIADICDTGF
                     VDGELIVPRFVLNELQFIADSSDSMKRSRGRRGLDVLNRMQKSTAINIEIVEQDFPKI
                     KGVDGKLVALAQKLNAKLLTNDYNLNKVAELQGVRVLNVNELANAMKPVVLPGEQMTV
                     KIIREGKEQGQGVGYLDDGTMIIVDSAQKMINMTVDVIVTSVLQTTAGRMIFTELKEA
                     ADRKSGNA"
     gene            21861..23051
                     /gene="ispD"
                     /locus_tag="PLQ77_03330"
     CDS             21861..23051
                     /gene="ispD"
                     /locus_tag="PLQ77_03330"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPL09743.1"
                     /translation="MKTVAIIPAGGSGRRAGASIAKQYLLLHGVPLLARTLRVFQNAE
                     AISAIVLVVPTPDVESVRERIVEKYSLTKVAAVVAGGGERQDSVASGLRAVPPDCDIV
                     MVHDAVRPFVTGEMIDRVAAAAACCGAASLGVPAKDTIKETTDDGIVTRTFPRSRLWQ
                     TQTPQAFACDLLRRAHASAKEDHYYGTDDASLVERIGAKVQMIAGSGENMKITTPEDM
                     KMAESLLCAGRGERIRRPSGLGYDSHRFAKDRKLVLGGVDIPFDRGLAGHSDADALLH
                     AVCDALLGMAGEGDIGRHFPDHDPAYKNISSLILLERVGKMVESRGVSIENIDATLVL
                     EKPRLAPYAQAMAANIARVLKIPETAVNIKAKTNEGMGFTGRGEGVAVWAVASGAERI
                     ADGE"
     gene            23041..24492
                     /gene="gltX"
                     /locus_tag="PLQ77_03335"
     CDS             23041..24492
                     /gene="gltX"
                     /locus_tag="PLQ77_03335"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPL09744.1"
                     /translation="MENKARVRFAPSPTGELHVGNARTALFNWMFARHEGGRFILRIE
                     DTDESRSDSAYQTSLLEDLKWLGLTWDEGPDIGGGYGPYRQSERLDLYEAHLKKLVDA
                     DLAYPCYCTEEELEEERQTLILAKRMPRYLGKCRNLSAGEREALAASGRKPSCRFKVP
                     RTTIEFNDLIRGPIRFEGEAMGDFIIVRSNGMPAYNFAVVIDDHRMAVTHVIRGEDHL
                     SNTALQIMLYRALGFEPPAFAHHCLILGKDHAKLSKRHGSVSVGEFRRQGFLPEALIN
                     YLGLLGSSFAGGREILSRDEMIDAFSLSRASRSGAVFDEDKLRWLNAAYIRKTSTEDL
                     LRKLEPFLRAAGFRPETLDAAWLKNVLETVKGELTTLAEIGAHIDLFFDERYSLSDEA
                     RRLLAAPGAREVVGAFGDYLERAEDNAGDIYAAAIRHAKEKSGAKGRDLFMPVRAALT
                     GKLKGPELDRVFAVLGVDSARGRFQQVKKTLGI"
     gene            24690..25316
                     /locus_tag="PLQ77_03340"
     CDS             24690..25316
                     /locus_tag="PLQ77_03340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09745.1"
                     /translation="MDSGKRNFDQDAARWDLNPARVKLASEIGSAILREISLLPDSDV
                     LDFGCGTGLLTLMLQPHVRSITGVDSSQGMLDILNRKISERNLTNVITRYADLDQGDV
                     LSGAFHLAVSSMTLHHIRDVAPLLKRFCHLLHPSGLLCIADLDEDGGRFHTSSEGVFH
                     FGFDRQKLARQFEEAGFQNVRAVQAASVEKPVAGTMQRFTVFLMIGRK"
     gene            25483..26841
                     /gene="radA"
                     /locus_tag="PLQ77_03345"
     CDS             25483..26841
                     /gene="radA"
                     /locus_tag="PLQ77_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPL09746.1"
                     /translation="MKKSKTSFFCQHCGYMSPKWLGKCPSCSGWNCFAEELVTEPDSV
                     SRADRVFGGKPMPIFDIPAVEGERTSTGIVEVDRVLGGGIVSGSAILIGGEPGIGKST
                     LMLQVMKNLAMGGRKVLYVSGEESASQIKLRADRIGAQAKDLLVLVEVSLEKILEQIR
                     KTEPAMVVIDSVQTVYSSDLMSAPGSVGQVREASSRLILAAKKSSIPVFLVGHVTKDG
                     SIAGPKVLEHMVDTVLYFEGDSGHAYRIVRSMKNRFGPANEIGVFEMRDRGLQEVPNP
                     SAFFLAERPENAPGSVVLASLEGTRPILVEIQALVSQSNLGMPRRTAIGVDHNRVSLL
                     VAVLDKICGLHLGGSDIFLNVAGGVRVEEPAVDLAVVSAMASSFLDRPVDARTVILGE
                     VGLTGEVRAVSQIETRVKEAARLGFNRCIVPKTNIASLGKAAKMEIQTITSLKELLDI
                     LF"
     gene            26973..27263
                     /gene="groES"
                     /locus_tag="PLQ77_03350"
     CDS             26973..27263
                     /gene="groES"
                     /locus_tag="PLQ77_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPL09747.1"
                     /translation="MKIRPLQDRIIVKRLEEETKTKGGIIIPDTAKEKPIEGKIIAVG
                     KGKVADDGKLIKPEVRVGDKVLFSKYGGTEVKIDGEEYLIMREDDILGIIEK"
     gene            27289..>28057
                     /gene="groEL"
                     /locus_tag="PLQ77_03355"
     CDS             27289..>28057
                     /gene="groEL"
                     /locus_tag="PLQ77_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPL09748.1"
                     /translation="MGAKILLYDEEARKSVLKGVNTLADAVKVTLGPKGRNVIIDKSF
                     GSPTVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATLLAQAIYRE
                     GVKAVAAGSNPMDVKRGIDKAVEVVVKELGKMSKPTKDQKEIAQVGTISANNDETIGS
                     IIAGAMDKVGKEGVITVEEAKGLETELEIVEGMQFDRGYLSPYFVTNPDKMIVALEDA
                     LILIYEKKISGMKDLLPILEQIAKMGRPLLIIAEDIEG"
BASE COUNT         5904 a         7722 c         8474 g         5957 t
ORIGIN      
        1 gggtgtccga ccccgtcgag ctgaattccc tgcacaccca gggatcggga aagattcgtc
       61 tggaagacgg aaaagtgctg accgtgagct acgcgcagaa caacggccgg ccgtttcgct
      121 ccgcgacgca gaaccttttg gatgaaaaca ggctggaccg gagcgacgcc tcctaccgcg
      181 ggttcaaagc ctggctcaaa ggcaagagcg aaaaagaaat ctatgaaatt ttaagccaca
      241 acgaacgcta catttttttc cgcttcgtgg accgcgaacc ggtcggttcg ctggggcagc
      301 cggtgacgcc tcaccggtcg attgccacgg acccggcgta ttttccgcag ggcgcgccgg
      361 cgttcatccg tttgcgaaaa ccggttctgg acgaggatta ccgtgtggtc cgccgcgtcg
      421 aattttcgcg ctttgtcctg aaccaggaca agggatccgc cattcggggc cccgggcgcg
      481 tcgatctgtt ctgcgggttc ggccccgaag cgcaggccac cgccggcagc ctcaaggaaa
      541 aaggcgaact cttctttctg ctgctcaaat aaattcgatc tttacttatc gccactgacc
      601 gggcgctttc cattttgacg ggacaggccg atcattaaaa cagatcagaa tatcttccgc
      661 ataaggatat gcatttggtc tggagcaacc atgccggcat ctcctaaagt tttcggttca
      721 ggtgagatac gtctgaaccg gcaaaatcat atcggatcat aatcatgctt gcagcaattc
      781 cccatcggga atattcatgc agcaagaagc atattcgcca taaacgattc cctatcggga
      841 atatattgct tgactgtggc gttataagcg gttattattc ccgaaaagga atgacggaga
      901 acaggaaagt tgaagatcaa agcagccacg gcggcaaata tcggcgcagc catcaggaaa
      961 aaacgcaagg aagacggact gactctggcc gatgcggcgg ctttatgcgg cgtcgggtac
     1021 agatttttgt ctgatctgga aaacggcaag ccgaccgcgc agatgggaaa agtcctgcag
     1081 gtgctgacgg cgctgggact cgatctttac attgcatcca gggagtggcc ggatgaataa
     1141 tcatatcggc aatccgggat tggatgtcta tctccgggat cagaaagttg ggcgcctgtg
     1201 gctggatgaa aaaagacgct ttgtgtttca atatgacgag gcctggatca gcaaaaaagg
     1261 cgccatacca ttatctttgc acctccccct gagagctgag acctatcctg atgatctgtc
     1321 ccgtcctttt ttttccaatc ttctgccgga agcggagatc aaacgaatca tcgctcagcg
     1381 tctgcaaatt tcagtaaaca atgactttgc catgctgggc agcatcggcg gcgaatgcgc
     1441 cggggcggta tccgtgctcc cctcaggcat tgcgcctgct gtgaaaccgg gttatcggga
     1501 acttaaagaa gaagaactgc acaagattat catcgacctg ccccgaaggc cgttgatggc
     1561 cggcgtggaa gggatgcggc tttccctggc cggagcgcaa aacaagctgc cggtttacat
     1621 ggaggacgac cgcatcttca tcgcctcggg aaatgcgcca agcacgcata ttctgaaacc
     1681 gccaatcagg gatctggagg atacggtaga aaatgaagct ttttgcatga tgctggctca
     1741 aaagatggcg ctgcccgctc cgccggttaa aatccgccgg gggcttgatc agcttttcat
     1801 catcgagcgt tatgaccgga gccgcgataa gagtggccaa atcgtgcgca tgcaccagga
     1861 agacttttgc caggcccttg gatttctgcc cgatcagaaa tacgaaagtg aaggcggccc
     1921 ttctctaaaa cactgtttta ctctgctaca ggaaaaaagc atacgccccg ctgccgaccg
     1981 catgaccctg ttgcgatggg tgatcttcaa cttcctgatc ggcaatgccg acgcccacgc
     2041 caagaatctg gccatgatct tcaccgaccg ggggccgcgc ctggcgccct tttacgatct
     2101 gatctgcacg caggtctatc cggatttaac ggaaaaggcg gccatgcgga taggcggcga
     2161 aaacaggccg tcctggatac agcagaaaca ctgggagaaa ctgggcgagg ccgttgcgat
     2221 caagccgggc cttattgtaa aaacatggaa agatatgtcc agcggcatca ttccaaaggc
     2281 gcaagtcatg gtgaatgatt tcaaaaaatc acacggaagc acaggaatca ttgaaaagat
     2341 aatggccgtc attgagaaac gggccggtga agtctgaaac aaaggtcagg ccaacccgcc
     2401 gagcctgaag aaaaacatgc acagtcctcc aacaaaaatt ggggtcgcaa gatccgggtt
     2461 gcccagcgtc ggcatatcgg caaagcgata cattccacgt cccagtcgct caatgacgcc
     2521 ttgatcgcgc atcttgtagc ggatccggga aggatggaaa tcaatgaatg agatcctttt
     2581 aggcctggcc atagccctgc agatcggcga cggagtgacg acctgttttg cccttcaaaa
     2641 gcagggcgtg agggaacgaa acgccattct gcgccggctg tttgacaggc tcggcgttgt
     2701 ccgcggactg gcgctggcca aaagcttcgg cgttgcggtt tgctggattg cctacgcgta
     2761 cggcgggccg tttgccgttt acactctggg cttcatcgtt gctttgtatg cggttgtcgt
     2821 gatcaataat cttaaatcgc tttgccggtg acgtttcccg ccacggtcgc aaagtcctgt
     2881 tgccgcacat ccccgatgct atgtgaaatg aacggccagc atggaaaagt cgtcttccag
     2941 gattttctgg ccgtggtagt cctgcatctt caggtacaga gcgtcaatgt cttcgcccgc
     3001 ttccattttg tgctgcagca gatattcccc cagttgatcc agcgtccaca tgctgccttc
     3061 gggaggatcc acttcgtaaa cgccgtcgga aaagacgtaa agattcgctc cggccggaac
     3121 agtgacggaa cttccctgat acgcgctttc cggaaggccg ccgatgaaca ggttgttcgt
     3181 catgagtttg gtcagggttc cctctttgga aatgagaaaa gccgggggat ggccgccgct
     3241 ggcaaagcga agttcccggg tccggcggtc gaaaacgccg taccagatgg tgaaataaag
     3301 gttgttgtgg tgctccatct gaaacgcgtt gttgagcgcg gcaagcaccc ggtccgggga
     3361 atggaaatcc gtgtcgggca gcgtctggga gcggatggcg ttgaaggcgg aaacggaaag
     3421 cagcgccgag ccgaccccgt gcccgcagac gtccagcagg taaacggcaa aggtgttttc
     3481 atcgagccag tggtagccga agctgtcgcc gcccagttgg gcgctgggga aaaagcgcca
     3541 tgccgtcgtg atcgtcggat cagacagcgg gggcggcatg agcgacacca cgtagtcggc
     3601 cgcctgggcc agctccgcgg ccagcgcctg ccggctggcg agcagcgcgg cataggcttc
     3661 gtttcgctgc agcaggttga tatagccccg ggaatggtac cggatgcggg cgacgagttc
     3721 gatgcggtcg ggaagcttca ccaggtagtc attggcgccg agcgcgaagg cgtccgcttt
     3781 cgtgaccgcc tcttccttgg aagataaaac gatcaggggc acgtccttga gctggggatg
     3841 gacgcggaaa aatttcacca gcgtcaggcc gtcgatgtcc ggcataatca ggtcctgaag
     3901 gatcaccgtc ggctgaaacg aggcggcggt ggggatggcc tgcgtggggt cctggcagaa
     3961 ctgaaaaacg atgtccgttt ccggctcgag cattcttttc accgccgcgc cgacaatcgc
     4021 ctgatcgtcg atgagcagga ccttgatttt gtggtccgtc agtatggttg ccgggtcccg
     4081 gcttccggac ggggcggggt tttgattttc tttttcggtc atgatggttt cccttttcgg
     4141 ttggtgattt tttttgcaat ggattcggcg atgctgtcca ggggcaggat ttctgaagcg
     4201 ccgcctagtt ccacggccgc cgccggcatg ccatagacga cggaggtctt ttcatcctgg
     4261 acgatcgtgt gccagccggt tttaaagaga gccgacaggc cttctccccc gtcccgcccc
     4321 atgccggtga ggaggatcgc cacgtcattt tgcggccagt attgccgaag actttcaaaa
     4381 agcgcgttga cggacggacg atagggataa tttcgggggt gctccgtgta gtgaagcgat
     4441 ccgtccggtc caaggacaag gtgatcgttg gtttgggcca ccagggcggc gccgggccgg
     4501 ggccgcatgc cttcgcgggc aatggctacg tcaagcggcg tctgggaatt cagccaggag
     4561 gcgaggcccg aggcaaactg cgaatcaaca tgctggcaga tgacgatcgc ggcgggaaaa
     4621 ttcccgggga ggccggcgag aatcgtggcc agggccttgg ggcctcccgt cgaagaaccg
     4681 atggcgacga ggggcggccg cggctctttt ttcgtttcat ggacggtcgc agccggtttt
     4741 tccattcggg acgcggttcc cagaagcctg gccatggtgt cgattttttt gagaagggcg
     4801 tctgcccctt ccagaaaccc gttggaatga agcacaggcg tggcaatgac atccagcgca
     4861 ccgcacccca tggcctcgaa tactttcccc gcctgccgtt ccctggcatt cgacaggacc
     4921 aggatcgcgc agggggtttc tttcatcacg tcacagatca gcttcgcgcc gtttacgtcg
     4981 gcaagctcca gatcgatgag cagaatatcc ggcagcccat gacggcattg ccggatgaca
     5041 tcgcttccgc ggcccgtggt ccagatgacc gcgcagccgg cattcggggc aatgattcgg
     5101 cgcaggattt tcagggcgtt ttcatcgtga tgggcaatgg cgattttgag cgtcattcgg
     5161 gtcctccgat caggtcgcga acggcggtaa gcagactttc gtcatgaaag ctgcccttgg
     5221 tcagatagta attggcgccg gcttccaggc cccgcagccg gtcttcttca cggtctttat
     5281 aggagacaat catgaccggc atgtcgtggt gctgcggatc ggctttgacc gtccggacaa
     5341 gatcgatgcc gttcatgcgc ggcatgtcca cgtcggaaac gagcagttca aaggcgtgcg
     5401 acgtcagctt gttccagccg tccacgccgt caatggcggt ggtgacctcg tatccggcgt
     5461 tttccagaag tctgcgctcc acttcgcgca cggtcaggga gtcgtccacc accagaacgc
     5521 gctttttgcc cgtcttcaga gcctgccggc cccggccgat tttctgaagc ttgccggtcg
     5581 tcagcaactg gtgaatggaa cggaccagat cgtccacatc gaaaatgacc accggcgaac
     5641 cgtcgtccag aatggcgccg gcgctgacat tggggacctt gccgaggcgg ctgtccagcg
     5701 gccggaccac gagattttcc tcgcccagca cgcggtcaac caccagcccg tagcggttca
     5761 gccggtcgct gatgatgacg acggaaaatc ccgcaggcgg ggtggaagcc tgcggcagcc
     5821 cgaaaagctg gcgcgcgtcg agaatgccca catgctcccc gtccaaagtg cagtagggtc
     5881 ggtcttcgat ttcccgaatg tcggcggggg ccagggacaa aacccggtcg atgcgggaaa
     5941 ggggcagagc gtacgcttcg ccggcaatcg acaaaagcag ggttctgagc acggaaagcg
     6001 tcagcggcag ctgcaggtga aagcgggcgc cccggccggc gtcggcatcc acgcgcacgg
     6061 ttccgcccac ttcatgggcc atgttgaaaa cgacgtcgag gccgacgccc cggccggaaa
     6121 tttccgttac cttgcgggcc gtggaaaacc ccggcagaaa cagaaactcg aagagctccg
     6181 cgtggcttaa gcttcccgcc atgtcctgcg acacatagcc tttttcaacc accttgcccc
     6241 ttaaatcctc gggattgatt ccccggccgt cgtcggtgag cgaaatgttc agcattcccg
     6301 cgacgtggcg cgcttccaga acgagggttc cttcggcagg tttgcccgcc gctttgcgtt
     6361 cttcgggtgt ttccatgccg tgatcgacgg cgttgcggat caaatgggac aggggcgcct
     6421 ccagacgctc cagaatatcc cgatcgacgg gcgtggcggc tccggcaatt tcgaagcgga
     6481 cctgtttccc cgtttcccgg gccagatccc ggaccagacg ggggaaaccg tggaggccgt
     6541 ccgagaaagg caccatgcgg ctgctgatga cttcgttgta aaggcgatgc gccagatgtt
     6601 ccagacggcg tgaaaagcgc tcgaaccgga caatttcccg ggaaacgatc tgctgggccg
     6661 agaccgcgcc ggacagcgtc tgctgtaagt cttccgctcc tttgtcctgc tcgtttgctt
     6721 cctgtcgctc caggaattcg cgcaggggtt tttcgatttc gaagatttcc tttttgagga
     6781 ccaggaggct ttgatagacg gttttgatgg attgggcctg cacgaggcat tcgcccgcca
     6841 gggccatcag acggttcatg ttttcggaac ggacgcgcac caggcctccg tctccctgat
     6901 cctgcgaacg gcgggaacgg acggccggcg gtgcggtttg cggctccggg catgcgtttt
     6961 ccaaaacggc tgccgggagc ggcttttgag gtgtgggctc ggcggggcgc ggtgccggcg
     7021 aagggtcggc tttaatgtct tgcgcctttt gggggacgtc cccggccgtt ttttcttccg
     7081 cctgcgcggc cgcggtagga gccggcgtgg tctcctgagg cgacgagaga aagtttctca
     7141 gctcgcctgc cataactgta atttcctggg ttccccgctc cagagcggcg ggcagctcct
     7201 gaatgtccag gcgggaaaga gaggcaaaaa aatcatttcc cttgagcagc cggtcaaccg
     7261 ctgtggaggt cagcggccgt tttccgctct gtgccgccga aagaacgtct tccatggcat
     7321 gggcgagggt gacggctgtg ttcaacccca caatccgggc tgcccccttg atggaatggg
     7381 cggcgcgcat cagcggttcg atgcgctccg gcgtctggtc gcgctctgcc gtgaccagac
     7441 cggcctccag catccgggtg ttgttttcca gctccacgcg gaacagatcg agcatcaggg
     7501 gatcgatggg ggctgcgtcc gtggtcatgg gttcagactc cttgtcatgg cggggaagag
     7561 ccgctcttca tccagaagcc cgaccgggct tccgtcaagc aggaaaattc cccgggtgag
     7621 ggctctcgcg cttcgtgaaa gtgtggccgg gacttccccg agatcctccg gggcaatccg
     7681 atgaaccccc agaatctgct cgacgggaaa cgcgaagcgc tgtccctcct gcccgaccac
     7741 cagcattcgc tcataagccg gcgcaggccc gctttcctgc gcctcgaagg tcaaatccag
     7801 aaggtccgcg acggacaggc acagaagcag ttctccgttg atgttggcga ttccccggaa
     7861 aatccggttg accttcaaag gcagtgtgtg aaccggggaa ggttccgccg cttcctgaaa
     7921 atagatggtt ttgacggcaa acttctctcc gcacaggcgg aaaaccatca cggacagcgt
     7981 tcccagcctg cctgtctctt tgggcaacgc catcacggcg gcccgctccc cgaggtatgc
     8041 cggcgggagg ttccggtcca gatgctgtct tcccgctgcg gcgaaaacgg ggcagcggcg
     8101 gcagtgaatc agacggggca gttccggaca ggatccgtcg ccgtagattc cttttgcctt
     8161 ccagcagtat ttttccgtgt tcgtggtcat tgttcctttt cggatccgtg cgatccgcct
     8221 ttatttcctg ccgttcgcag ggcgcgctcc cggtaaagcg ccgctttttt ttcgtttccc
     8281 ttctgccggc agatcaggct gatgtggacc aggctttcgt agtggccggg atcgaggtag
     8341 agggccttca aaaaatagtc ctccgcgccg ttcgaatcct gctcggccat ggagaggatt
     8401 cccatcaggc aataggcttc ggcggccggt ccgaatttgc gcgtgcattc ccggcagaga
     8461 cgggcggcgc ccttcatgtc gcctgtgtcg gccagccgcc gggcctcctg aatctgctcg
     8521 tccgcctgcg aaagtttgaa attttcactt gcggtttcgg attttgccgg ctgcccccgt
     8581 cccttgcccg cagtcttccc ctgaccggtg tttgaagggg acgatgttgc ttcctgcgcc
     8641 gcggcgccgg ttttgcgggg gaagttttcg cccggtcgga ccgacggttt tgtcggggct
     8701 tttgtcctga ttgccgcggg ctgagccggg gaagcgggct tggccagggc aaaggccctg
     8761 tcccacggga ggagctgata gccgcgctga tgccagaaat ttgtttccgt atggccggtg
     8821 aagagcagac cgccggggat cagaagcctg ttcatccggt caaaaatccg aagacgggcc
     8881 tcgggggtca tgtagatgag aaggtttctg cagaaaataa tgtcgtacgg accgcgtccg
     8941 gcaaggcttc cgggcagcgc caggttctcc ggtgaaaagg agaccaggct tgcgacggcc
     9001 tcggcgactt tgcggcccga aggcgtcgtt tcgaaaaagc gggcgacatc ctcgccgatg
     9061 cttccccgga aagcgctttt cgggtagcag gcgcggccgg ccgtctcgag ggcccgcgcg
     9121 ctcgcgtcca cgccttccac cgtgaaggcc gaaggcggca ggccggcggc cagcagggtc
     9181 atgacgatgg agtagggctc ctcgccggtt gcgcaggggg acgacagaat cttcaaggtg
     9241 cttcccggat gcgtctccag ccattggcgg gcgtggcggg caagccagag aaaaggcccc
     9301 cggtcccgga aaaaccaggt ttccccgacg acgacggcgt caatcagctt ctgccttgcg
     9361 gcgggagagg acaaaaacag cttttcatac tcccgcgcat ccgaaacgcc ttcctgacgc
     9421 atggcgaaag cgacggcgcg gcgcacggca tcttttccgg cggagccctg ctccatgccc
     9481 agggaatgcg tgatgaattc catgacggcg gcaggcgcgt tcatccgttg gtgacctccg
     9541 gcggaaaaag agtgaacagc aggtcgcggg tcagcagccg ttccatgtcc agcacctgaa
     9601 gggagccccg ctcgtcaaat atcgtatccc ccagataggc cgcgccgtcg acgccgacca
     9661 ccggctgctg aaggttttcc tttttgcatt cgatcatctc cgtcaccgcc tcggccagaa
     9721 gacccagaat gtggaagcgg ccgtcttccc cctgatagtt gacgagaata atgcgcgtgc
     9781 tcattcgggg acgggatgga gagccggtca gaagcacggt caggtccacc accggggtga
     9841 gcgttccccg gtaattgaac gttcccgcga cttccggcgg cgcgtgagga acggccctca
     9901 gggaaaccat ggaggatact tcaataatct gtccgatatc gaggccgtaa cggtccgtgc
     9961 ctgcctgaaa ctgaagtagc aacataggtt gtttcccgat caggagctga ttttaaaccg
    10021 ggagacctct gactgaagcc ccgcgatggc ctcgttgagc tgctccgttg ccagcttgaa
    10081 ttcgtgcagc gacgccttgg tctgctccgc cgccagcgac aactggccca tggcttcgct
    10141 gatctgctgc gcgccctggg actggccgta catgccgtcc ttgacggcgg tgaactgcgg
    10201 cccgtggatg cgcacctgat caatgatttg ggcaagctgc ccgctctgcg cggccatggt
    10261 ttccacgccg cgccggacct cgtcgttgaa tttgtccatc tccatcacgc cggaggcgac
    10321 ggacgtctgc atttcagaga cgacctgctg gatgtcctgc gtggcgatgg ccgtctgatc
    10381 ggccagccgg ctgatctcgc gggcgacgac cgaaaagccc cttccgaatt cgccggcttt
    10441 ttcggcctcg atggcggcgt ttaacgacag cagattggtc tggtcggaaa tcttgttgat
    10501 ggtggtgacg acatgggaga tccggttggc ccggtcgttg atgacggtca gctttgaaga
    10561 gatggaggac gtggccttga ccagttcccg catggccgtt tccatccggg aaaggtcgct
    10621 gcggccctcc tgggccatgc cgatggtttc gttgagcgat tcattgaccc cgtccatggt
    10681 ctgggccagt tcgtcggccg tggcggagat ttcccggctg gtggccgtta cttcggtggt
    10741 ggaagccgcc tgctgcgcca cggtcgcttc gagttcgcgc gccgaagcgg cgatttccgt
    10801 ggcggacgtg gtgacctgga tgcccgagcg gtgaacctgg ccgatgaggg agtcgagatt
    10861 tcccgtcatg gcctggaagg cctggagcag tttcccggtt tcgtctccgg cctgaatgac
    10921 ccgcgttgac gcggttcccg ccagatcttc gcgggcctgc tgaatatcgc cggtagcgat
    10981 cttctgggca agcgatgtcg tcagttccag aggggccgtc agccgacggg ccaggacagc
    11041 ggccagcaga accgaaagaa agatgatcag cgcccccgcc aggagcattt tcagaaagat
    11101 ggacttgatc atgtggtgca cgtgtccgat ggggcggaag aagtcatctt cataggttcc
    11161 ggccacgatc acccagtcga agggttcaaa gtaaatgacg gacgatatct tttttctcgg
    11221 ctcgggctcg ccgggattct gccagatgta ttcatggtag aaggattcgc ctttgggctg
    11281 ggccagcgcc ttggtgatca gggtctgaat gatgaggttg ccgtggacgt ctttctgatc
    11341 ccagatgttt tctccgtccc gcttgccgtc tttggaaatg atgtagcgcc cccggtgctc
    11401 gttcttgccg ccgacgacgc ccacatagcc cagctttccc accttcatgc tcatgatcgc
    11461 ttttcgaagc gaagcgacgg cttccagctt ttcccccacg tacagcatgc cgatgatttt
    11521 accgccgcgg tccgtgacgg gctcataggc ggtgaggtac cacgtgttga ccacataggc
    11581 gagaccccgg taggactgtc ctttcaggac cgccgccgtc accgggttgg cgctcccgtc
    11641 cggcagcgtg tttggaatat aggtgccgat ggcccggcga tggttcaggt cttccacgtt
    11701 ggtggccacc ctcagcatgt cgccgttttc gttcatgcgc tgaaaaatgg tgcaggtgat
    11761 gccgaccaga cgcttcacgt catcgacgat gggcgtgggc gtagaagtgc tgcggttctg
    11821 tcccagccag acgccgcccg ccgtcagttt gggaagctcg attttctggg tttcccgggt
    11881 caactggttt acggcatccc atgcgatctt ttccctggcg gtttcgatgc ccccgtgctg
    11941 cttcaggatg cttctggcta cattcagccc gttgttgatt tttcgctgga tcaggtcgtt
    12001 ggaagtttcg cacagtccgt acacgtccct ggagatctgg gacatgttgg ccattgcaac
    12061 gtcgctaagc tctttcgagg ccaggtcgga aacggaggtt ttgaaagatc ccatgagaat
    12121 catcatgacg gcgaccggca gaacggcggc catgacggca aggccgatga tctttcgctt
    12181 cagagaaagt tttatcatgg cgacgcatcc ttgattggta tttttgcgcg gcaaagtata
    12241 gggaacgccg tggggtctgt caagaaaatc ttggaaaatt cagggggaag tccgccgacc
    12301 tgttgccgga tggccgggaa agacattccc cgcgcatatt atgtattgac aggatgaagg
    12361 gattgcagtt atccccgtca tgtccggaaa aacgaaaaaa ggaattgcgt tttcaaacgc
    12421 ggagcgtgaa gggggccggt ttgttcacac ggcggatttg ccggataggg aagccggcgg
    12481 gaaattccat gagcggcggt gcggcgaaat gatggacgaa gtgacgattg acgaaatcct
    12541 gggcggacgc ctcaaaatcc gccagagcaa aaggggctac cggtttaacc tggattctct
    12601 gatgctggcc ggttttgcct ccatcaaacc ccggtccgtg aatctggacc tgggctgcgg
    12661 caacggaatt ctcgcgctgg ttctggccgg aaggcatccc ctgacaagat gggtcggcct
    12721 ggagattcag gagggcctgg caaatcttgc ccgcgccaat gtggaacaaa acggccttgc
    12781 ggggcgaatc tcgattgtcg cgggcgacgc caggaatgcg gggaaaatct tttcaaacca
    12841 ttcgtttgac caggttgttt tcaatcctcc ctatcggaaa atttcctccg gccgaatcaa
    12901 tccgctctcc gaaaaagcga ttgcccgcca cgagcttcag ggggcgctgg cggattttct
    12961 ggcggcgtca aagattctgc tgaagccgaa aggacgggtc ttcacgattt atccggcggc
    13021 ccggctggtg gagctggtca gcctgtttcg ggccaggggc atcgaaccca agagaatgaa
    13081 gctggttttt tccgacgcgt catccgatgc ggtcttcgtg ctggcggagg ggcgaagcgg
    13141 ttccggtgaa gagctgaaga tggagccgcc gctttacata tatgaagagg ggaaaaccta
    13201 cacgccggag atgaaaaagt tgttcagcga tcttgccgct tttcccgaag cctgcggcgg
    13261 ctgatttccg cctgcatgac cctttttaca acggcggcca gttcgggatc tttgagcgtt
    13321 tcggtgcatt cggcgatgat tcttttgtct ctctctttca gggcagtccc gggcgggacg
    13381 gccagccggc ccgcgtcccc gcggggaggg ctgtaaccca cgccaaagtg gatttcctgc
    13441 acgaccgttt ttccggccag atcattgatt tttttccgga tgtcttcttt catgaaatga
    13501 agctgctgca cccagaccga ggaggtcgtt ttcacgaaga gggtgcccgc gcgccatccg
    13561 tcgggacggg tttgccgggc gattttcgcg ccgaccgcct gcggccagag cttgaagacg
    13621 ggattgtccg ccagtttgcc ggccaggcct cttctcttca gggcggaaga aagaatgtct
    13681 cccaccggct gcaattgacc tttaaagcgt cttctcatgg ctttgaactc ccgcattgcg
    13741 cggcgggatc cggtttttcc agaaccaccc gcaatttccc ggccaaatcg ctgatcgtga
    13801 aaggcttctg aatgaagccg ctgcagccgc gttccatgat tttgccggcc tggccgttca
    13861 ggctgtagcc gctggacagt aaaaccctga cgcccggatt gatccgccgg atggcgtcga
    13921 agacctctga cccgttcatg tccggcatga ccatatccag aatgaccaga tcaacgcgct
    13981 cctgctcccg ccggtagagg tcaatcgcgg cggcgccgtt gggaacggtg attacccggt
    14041 agttcaaggt tctcagcatt tcgccggcca catccagaac catggcttca tcgtccacga
    14101 ccaggatcgt ttccctgtcg gaggatctct ccggggcgga cgcttcttcc ggacattgat
    14161 ggttttggga ctggtgtgcc gaggccggca aataaatatt gaaggtcgag ccctgccctt
    14221 tttcggaata gacgttgatg aagccgttgt gatttttaat gatgccgtaa acggacgcca
    14281 ggcccagccc ggtgcccctc cccatttccc gggtggtgaa aaacggctca aaaattcttt
    14341 cccgggtttt ctcgtccatt ccgacgccgg tgtccgtcac ggagatcttg acatagcggc
    14401 cgcctttgcc gctgaagtct ttgaagcgat tttcgtccac gatcatgttc ttcgtttcca
    14461 ggtgcagatc ccccccgacg ggcatggcct gccaggcgtt gatgaacaga ttcaccagca
    14521 cctgctcaat ctgcgccgga tcgatttccg tcatccagag cgggtcttcg aagtggtgat
    14581 gaatgttgat ttcctttttg gtgcggccca tggtccggga cgtctgatga atcaggtcgt
    14641 tcagatcgac gcttttgact tcatgctttc cgccgcgggc aaagccgagc agctgcctgg
    14701 tcagaccggc gccgctgacg accagggttt cgatggtccg gatatgttcg taattgggat
    14761 caccggggtg aaggttcaaa agcgccagag aggcatggcc ctgaatgccc atgagcagat
    14821 tgttgaaatc atgagcgatg cctccggcca gcgtaccgat ggattccatt ttttgcgcct
    14881 gcagcagctt ggcctccgcc tttttttgcc gggtaatgtc gcgcatgaag ttcagcgtcg
    14941 ccggagcgtt ttcccagcgc atcaggacac tgttggcggc cacccagatg gtcgattcgt
    15001 cccggcggac catccggaaa cggttgttca acgacggttc gctttcctga agaacggctt
    15061 gctgccgctc gtcgatcata tcgcgatcct cggcaatcac caggtccagg aaaggcatgg
    15121 cctgaagatc ggccagcgga taccccagca tcttttccgt gcggctgttg gcaaagccga
    15181 ttttcccgtt cgcgatgatg aaaatcgcct cgctggaata ttcgatcaac tggcggtatt
    15241 tttcctcgga ttttcgcagg gcttcttccg cttcccttct cggggtgatg tctttggaaa
    15301 ccaccgccac ggcgatgatg ggttcggggc tggaggaatc ccgcaccggg ctgaaggtgc
    15361 gaagaaaata ccggccgtcc cgcgcgctct gatgctcgtc gaaagcggac tggcccgttt
    15421 ggtaaacgct tttgatttta agctcgaatt tggcgttctg ctcgggcgag tgaatgtcct
    15481 tgtaggaaag gccggcgaca tcgttttgcg gcattttcag gcgcctggca tggttctggt
    15541 tgataaacag gtagcggcag tcggcatcca ccaggtacag ggaatcgctg gtggagtcca
    15601 ccaggttgcg gtattgattt tcgacagcct gctgttcgtt cactttccgg gacagcaggg
    15661 cgactttctg ctccagttcc tcgtaggtcg gtttagcgtt catgaaggtt cccgcgcctc
    15721 tcacgtcatt aattttttca actttccggc gaaaatgtca taggtccgct catcaaataa
    15781 aacatggcga atcagcttca gctccgcgct gctgcgcgca aactcgaggc aggtttgaag
    15841 cgccagggag gcggcttcgt gcaccgggta tccgtaaacc ccgcagctga tggccggaaa
    15901 ggagatgttg ccgacgcctt ttttgacggc cagcttgagg ctgtttcggt acgccgaagc
    15961 cagcagggcc gcctcgcctc gtcctccgcc gcggtacacg gggccgacgg tatggatgac
    16021 atagcgggct ttgagattgc cgccggtcgt gatgacggcc tcgccgacgg gacagccgcc
    16081 gattttgcgg cattcggcca tgatggcggg tcctccggcg cggtgaatgg ccccgtcaac
    16141 gcccgcgccg cccgaaagtg tgctgttggc ggcgttgacg atggcgtcgg tttcttcctc
    16201 ggtgatgtct gcgcaaacga tttcgatcac ggtttggttc actgtgattt gcatgctcat
    16261 tcctccgcgg ggatgggggt tccctctccg taaaccagca tccggtaggt tttttcgata
    16321 cggtcccagt tttcttcgcg gtccatcgac cagtatcgtc cgatcacgga cacgacctgc
    16381 ccgattccca gcgagcggca tttttcctcc acctgccgaa tatagcgcgc gccgctttcg
    16441 gcctgctcgc cgcgccgccc cagcatggaa tgaagataca gctcggagac gccctgctct
    16501 gcagccatgt ccatcagggc aaacagatgt tcgatggacc cgtgcgagct gaaaaaagac
    16561 acgatgccca tgaggtgcag cgcacggcgg ttttgccggg ccaggttcat ggcgcgcgtg
    16621 aaagccgggt ttcggtgaaa atccctgcgg gcaatcgact gatcgatcat caggcggtcg
    16681 gaatagatcc gccggcccgc gccgatgtgc agatgccccg cttcggagtt gcccacggtc
    16741 ccggcgggaa gccccacggc caaaccggag gcggccagcc gcgcatgggg gtaatcccga
    16801 agcagccggt ccatcaccgg cgtttcggcg caggcgatca ggttgccctt ttggctgtcg
    16861 caaagccccc agccgtctaa aataagcaga agcagacggg aagcgggggc gagggaagga
    16921 ggcttttcct gaatcaaaga cgatccggtc atggaatcgg ggacgggcag gcccagcaaa
    16981 tccagaatcg tcggcgcgat gtcggtcagt tctccttcgg gtttcagggc gggtttttgt
    17041 tccgggtgca gaagaatgaa gggcacgggg ctgtccgtgt gtccggtatc cacgccgccg
    17101 tccggataca gccatttttc cacggtgccg tgatccgcgg tgacgatgac ggtcatgttc
    17161 cggcgaagcg cctgctccac gagcaatccg gcgtgccggt ccaccgccga aaccgcccga
    17221 aggatcgccg gttcgttttc aatatggccg acgacgtcca cgttcgggaa gttgacgacg
    17281 atgaaacggc aggaggggtc ctgaattttg gcccctgccg cgcgcgtgat ttcatcgatg
    17341 gacatttcgg gcgcttcgtc gaacagcctg acgtccttgc gcgtcgggac aacgattcgt
    17401 tcttctttcg gcaggggatc gcttttcttc ccgttgaaga aatagctgac atgcacggct
    17461 ttttccgctt ccgttatttt tgcctgcgcg aggccgtgcg ctgcgatgat gtcgctcaga
    17521 gtgtcggaaa ggacgccctg gggaggaaat gcgacgtcgg cgttcagccc tttggaatac
    17581 tcgatcatgg tggcgaagtg cacggggagc gccgggtgaa cggggaattc ccggaagttt
    17641 tcttccgtga agctgcgggt cagttcaatt tcgcgctctc cgcgaatatt gtagaaaatg
    17701 atggagtcac ccggctggat tcttcccagc ggggcctgct tttgatcgac cagaaccagc
    17761 ggaaggagcg tttcgtcttc ctttccctgc gcgtaggcgc caaggacggc ccgggacatg
    17821 ggaaatgtct tttcagagct catcgaaaat tccggctaaa cgttgaatga tatcgggcag
    17881 gctatgtaac gaaataatgt cgaactgtaa aggaaaaaaa tatccgtccg gcgggccatg
    17941 agcgatttga acttgaaaat taagcgcttg aatgatagag gccctcagaa atcgttaaga
    18001 aggctctatg gttcaggtac tgctcaaagg aagagaaaaa cagcaggaaa atttgtatga
    18061 agaactgttg cgggagaggg cggcggttct tgccagggcg ggttttgccg tcgaggacgc
    18121 gcttgagaaa ctggagaagc ttgaccggcg catcggagaa atgatgaacc gctgccagtc
    18181 gctgcaaacc ggctcgcctg tcggtgcgca tccgcgggaa cagcagtcgg tttttgaaga
    18241 aatcgacgaa atcattgatc aatttaacgc aacctgccaa aaagccgagc ttcagtatta
    18301 ttatttgatc gtgacccgtg aagcccttgg gttgcgccgt cacgaaacgg tgcagaaatt
    18361 ctaccggatt ccctctaaaa agaaaaagat gcgagcgctt taatggacag gtataaaaaa
    18421 cagcgcattc gaatggtgga agcgcagatt cgcgcccgtg ccgtacggga cgaaagcgtt
    18481 ttgaaggtaa tggagacggt tccgcgtcac ctttttgtgg acgaagggct gattaaccag
    18541 gcctacagcg acaatccgct gcccatcggg gagcggcaga cgatttctca accctacatt
    18601 gtcgccctga tgacgcaggc cctggagctc aaaggcatgg agcgggttct ggaaatcggc
    18661 acgggatccg gctaccagac ggccattctg gccaaactgg ccgaccgggt cttttccatc
    18721 gagcgcgtgg ccgtgctggc ctcgcgggcc cgccgaatcc tggaccagct gaattgctac
    18781 aatgtcgcca ttcgcgtcgg cgacgggtcc tacgggtgga aggaggaggc gcctttcgac
    18841 gcgatcatta cgaccgccgc cgccccgcag cttccccggc atctgctcga tcagcttgct
    18901 gtcgggggca gaatggtggt gccggttggc ggccgggagg cgcagacgct ttataaaatt
    18961 acccgctcgt ccgaaaatcc gtccgagctg aaaagggaag acctgggggg ctgccgcttt
    19021 gtcagcctga tcggagagag cggatggaaa gaatgagccg gcacattctc ccccgccggc
    19081 attttgccgt tttcagacgc cggtattttg gggcgtactt gattttgtcc atgctggtgc
    19141 ttctgatgcc cgccgggtgc gcgaattccc aggtgcacag gagccaggcc agcggcgttt
    19201 atcacatcgt caaaaagggg gaaaccctgt acggcatcgc ccgcgcttat tcagccagcc
    19261 tgcaggacgt ggcggaaatc aataacatcg aggatgtctc caacatccgc gaaggggccg
    19321 tcatttttat tcccaacgcc gatcaggtga tcgaagacgt tttggtatcc tcggggaaag
    19381 ccgccgcgga cgagaaacca tccgtcagga aaccgcctcc cgtttccgcc gaagttttgc
    19441 cgccgccggg aaaaatcccc gcaaaaccgg ccaaaccatc ccgaccggag gtccaatccg
    19501 ttccgcccgg accgaaatcg gaaaaaacaa aagcgcccgt tgaggaaaaa ccgcaaattt
    19561 cggaagaaaa agtggtgatg gataaaggcc ggtttgcgtg gcccgccagg ggatccgtga
    19621 aaacccgctt tggaattcag ccgaacaaaa cctaccataa ctggatcaaa atcgcctgtc
    19681 cggccgggac aagggtgaaa gcggcggcct tcgggaccgt tattttctcg gccaatttaa
    19741 aggatttcgg cgaaaccgtg attatccggc atgagaaaga cttcgcgaca gtctatacgc
    19801 acctgaaaaa aagatccgtc caggcggacc aggatgtggg gattggccag accattggac
    19861 tcgccggaag agccgatgaa gcgggggaaa cgtacttcaa ttttgaaatc cgcctcaagg
    19921 gcaaagcccg gaatcccctg ttctatctgc cttgaatgac cgccgtttag gcctctccaa
    19981 aacatttctg aagtatttca gtaaattgta ttaatcattg actaaatcaa gcaaaagtga
    20041 taatgttcaa cgttcttaag aaaggcaggg aaatgtccaa tgtttaaagt aggtgatttg
    20101 gccgtttatc cggctcaggg cgttggcgtc attgaagcta ttgaaaatag agaggttatg
    20161 ggaaagaaac agccctttta tgtgatgaaa atcatgggca acggcatgaa gatcatgatt
    20221 ccgaccaatt ccgccaaggc ggttgggctc agggaagtca ttctggagaa agaaataccc
    20281 aaggtttacg agatcctgcg caacaaagac gttaccatag acaaacaaac ctggaacaag
    20341 cgataccggg agtacctgga gaagatcaaa accgggtctg ttttcgaaat tgcaagtgtt
    20401 ttaagggact tgttcatttt aaaaagcgac aaaaaccttt ctttcggcga gcggaagatg
    20461 atggataccg caaaaaatct gctcgtaaag gaaatttccg tcgccacgaa cgcgcaggaa
    20521 gccaaggttg aacgcgattt gcagatgatt ttcacggtgc agtagcaagc gggtgcgtgg
    20581 tctgtctctt tttcgctctt tgaaaactga aaatggacaa accctatttc cctgaatcgt
    20641 ttacggcccc tccacggtca acaagcagta cggaagcatg attcgctcat ttcattcctg
    20701 attcatcatt atccattgaa tttattttga agggaggtga tacgattgat cgtaagagct
    20761 ttactgattt tgatttgttc ggttagcggt tatttcatcg cctaccacac gatggcggca
    20821 gccggctcct acgctgtttt tcaggcaatt ttcggcttaa ttataggctt ggtcgttgcg
    20881 atgctggtta ttcggatcga acgagatatc cgcagacagt ccgtaaggac catcgcaggc
    20941 ggcgtagccg gcatgatcac cggcctcctg atcgcgctga tcatcggctt tggattgaac
    21001 atgatcatcc gaattccgcc caatcagcag attgttccat ggatttacct gcttatgatc
    21061 ggcattctgg ggtacctggg actggtgatc ggctcgaaaa aggcggagga aatcagcttc
    21121 cgcactacgg attcgaggaa gctgatggat caccgcctgc tggacaccag cgtgatcatc
    21181 gacggaagaa tcgcggacat ctgcgatacg ggtttcgttg acggagagct gatcgttccg
    21241 cgttttgtct tgaacgagct gcagtttatc gcggattctt cggattccat gaagcgctcc
    21301 aggggccgcc gcggactgga tgttctcaac cggatgcaga aaagcacggc catcaatatt
    21361 gaaattgtcg agcaggattt tcccaaaatt aaaggcgtgg acgggaaact ggtggccctg
    21421 gctcagaaat tgaacgccaa actgctgacc aacgactaca acctgaacaa ggttgccgaa
    21481 ctgcagggcg tgcgcgtcct caacgtcaac gaactggcca acgccatgaa gccggtggtc
    21541 ctgcccggag agcagatgac ggtgaaaatt atccgggaag gcaaggaaca gggccagggc
    21601 gtcggctatc tggacgacgg gaccatgatt atcgtggaca gcgcgcagaa aatgatcaac
    21661 atgaccgtcg atgtcatcgt gaccagcgtg ctgcagacga cggccggccg aatgatcttc
    21721 acggagctga aggaagccgc cgacaggaaa agcggcaatg cataggcatc tttaagcgca
    21781 ggttttttgg ccgggaagaa agatatcctg aacaggcccg gaaaatggtt ttccgggcct
    21841 gtttttgcaa gagattcgct atgaaaaccg tggccattat tcccgccggc ggctcaggca
    21901 gaagggccgg cgcatcaatc gccaagcagt acctcctgct ccacggggtg cccctgctgg
    21961 cgcgcacgct tcgcgtgttt cagaacgcgg aggcaatttc cgccatcgtg ctggtcgtgc
    22021 cgacgccgga tgtcgaatcc gtccgggagc ggatcgtgga gaagtacagc ctcaccaaag
    22081 tggccgccgt tgtcgccgga ggcggggagc gtcaggattc cgtcgccagc ggcctgcggg
    22141 ccgttccgcc ggactgcgac atcgtcatgg ttcacgacgc cgtaaggccg tttgtcacgg
    22201 gggaaatgat cgaccgggtt gcggccgcgg cagcctgttg cggcgccgct tcgctgggcg
    22261 tcccggcgaa agatacgatc aaggaaacga ccgatgacgg catcgtcacg agaacgtttc
    22321 cccggagccg tttgtggcag acgcagacgc cccaggcgtt tgcctgcgac ctgttgcgcc
    22381 gcgcgcatgc gtcggcaaaa gaggatcact attacggaac ggatgatgcg tcactggtgg
    22441 aaagaatcgg cgccaaagtc caaatgatcg cgggatccgg tgaaaatatg aaaattacaa
    22501 cgcccgaaga tatgaagatg gcggagtccc tgctctgcgc cggaagagga gagcggatcc
    22561 gccgcccaag cggattgggt tacgacagcc atcggttcgc gaaagaccgg aaactagtcc
    22621 tgggcggcgt ggacattccc tttgaccggg ggcttgcggg gcactccgat gccgacgcgc
    22681 ttctgcatgc cgtctgtgac gcgcttctgg gcatggccgg tgaaggagac atcggccggc
    22741 attttccgga tcacgatccc gcttataaaa atatttccag cctcatcctt ttggaacggg
    22801 tcggaaagat ggtcgagtcc agaggtgtga gcattgagaa cattgacgcg actctcgtgc
    22861 tggagaagcc caggctggcg ccctacgcgc aggccatggc ggccaacatc gcccgtgtac
    22921 tgaagattcc ggaaacggcc gtgaacatca aagccaagac caacgaggga atgggcttta
    22981 cgggccgcgg agagggagtg gccgtgtggg cggtggcctc aggcgcggaa aggatcgcgg
    23041 atggagaata aggccagagt ccgttttgct ccgtctccca ccggagagct gcatgtgggc
    23101 aatgcgcgca ccgctttgtt caactggatg ttcgcccggc acgaaggcgg ccgcttcatt
    23161 cttcggattg aagacacgga tgaatcccgc agcgattcgg cctaccagac cagtctgctg
    23221 gaggacttga aatggctggg cctcacctgg gacgaaggcc cggacatcgg cggcggctat
    23281 ggcccttacc gtcagagtga gcggctggac ctctacgagg cacaccttaa aaaactggtg
    23341 gacgcggacc tggcgtaccc ctgctactgc acggaagaag agctggaaga ggagcgccag
    23401 acgctgattc tcgccaagcg catgccgcgc tacctgggga aatgccgcaa tctcagcgca
    23461 ggcgaacgcg aagcgctggc cgcgtccggc cggaagcctt cctgccgttt caaggttccc
    23521 cggacaacga tcgaatttaa tgacctgatc cgcgggccga tccggtttga aggcgaagcc
    23581 atgggcgatt ttatcatcgt gcggtccaac ggaatgcccg cctacaactt tgccgtagtc
    23641 atcgacgacc accggatggc ggtcacgcat gtcatccgcg gcgaagacca tttgtccaat
    23701 acggccctgc agatcatgct gtatcgggcc ttaggatttg agccgccggc ttttgcccac
    23761 cattgtctga ttctgggcaa ggaccacgcc aaactcagca agcgccacgg ttccgtatcc
    23821 gtgggcgaat tccgcagaca gggatttctg cccgaagcgc tgattaatta tctcgggctt
    23881 ctgggaagtt cctttgccgg aggaagggaa atcctttcgc gcgacgaaat gatcgatgcg
    23941 ttttctctct ctcgggcgtc cagaagcggc gcggtctttg atgaagacaa actccggtgg
    24001 ctcaacgccg cctacatccg caaaacctca acggaagatc tgctcaggaa gcttgagcct
    24061 tttttgcgcg ccgcgggctt tcggccggaa acgcttgacg ccgcctggct taaaaacgtt
    24121 ctggaaaccg tgaaaggtga attgacgacg ctcgcggaga tcggcgctca catcgatctg
    24181 tttttcgatg aacgctattc cctgtctgat gaagctcgaa ggctgctggc ggcgccgggc
    24241 gcccgcgagg tggtcggggc tttcggcgat tatctggagc gggccgaaga caatgccggg
    24301 gatatttacg ccgcggcgat ccggcacgca aaggaaaaat ccggcgcaaa aggaagggat
    24361 ttgttcatgc ccgtccgggc ggcgctgacc ggaaaattga aaggtccgga actcgaccgg
    24421 gtttttgccg ttctgggcgt ggattccgcc cgcggcagat ttcagcaggt gaaaaaaaca
    24481 ttgggcatct gaaagggcgg cgttcgcatt cgctggtttg ccttttattg ttttcttcgg
    24541 gttgccggat catccccgaa cccgcctcaa agaaagccga tcacccgctg aaacagttcg
    24601 tctgcctcct gagctgatga accacggcaa atcataacca atgctgatga cgatttcggg
    24661 gcggatttac cggagcgtgg aagaagggca tggatagcgg gaagcggaat tttgatcagg
    24721 atgcggcgcg gtgggatttg aatcccgcaa gggtaaaact ggcctcggag atcggcagcg
    24781 ctattcttcg ggaaatttct ctgttgcctg atagcgatgt ccttgatttc ggctgcggaa
    24841 cgggcctcct cacgctgatg cttcagccgc atgtccgctc catcaccggc gtggacagct
    24901 cacaaggcat gctggacatt ttaaaccgga aaatatccga aaggaatctc accaatgtca
    24961 tcacccgtta tgcggatttg gatcaggggg atgtcctttc cggggcattt catctggcgg
    25021 tcagcagcat gacccttcac cacatccggg atgtggcgcc gcttttaaaa cgcttctgcc
    25081 atcttctgca tccttccggc ctcttgtgca tcgcggatct ggacgaagac ggcgggcggt
    25141 ttcacacctc cagcgaaggc gtcttccatt tcggctttga ccgacaaaag cttgcccggc
    25201 agttcgaaga ggctggattt caaaacgtcc gcgccgtcca ggcggcttcg gttgagaagc
    25261 cggttgcggg cacaatgcag cgcttcaccg tttttttgat gatcggaagg aaataggcgc
    25321 cgcgggcgtt tgccggttgc cggtcaggag aaacggcagg gccgtcattt ccgtaagcgc
    25381 agacggcgaa ggatggctca ggcaacgccc ccgcccaatt cctgttggcg ttttggaaaa
    25441 aaatgtgttc agatgtcaag ccgaatcaag gagaaaagca ttttgaaaaa gagcaagacc
    25501 agcttcttct gtcagcactg cggatacatg tcgcccaagt ggctgggaaa gtgtccgtcc
    25561 tgcagcggct ggaactgttt tgccgaggag ctggtgacgg aaccggattc ggtcagccgc
    25621 gccgatcggg tgttcggggg caagcccatg cccattttcg atattcccgc cgttgaggga
    25681 gagcgaacgt ccaccggcat tgttgaagtt gaccgggtgc tgggcggcgg catcgtcagc
    25741 ggttcggcca tcctcatcgg cggcgagccg ggcatcggca aatcgacgct gatgctgcag
    25801 gtaatgaaga atctggcgat gggcgggcgg aaagtgctgt acgtttccgg cgaggagtcc
    25861 gcctcccaga tcaagctgcg agccgaccgg atcggcgcgc aggcgaagga tctcctggtg
    25921 ctggtggaag tgtccctgga gaaaatcctg gagcagattc gaaaaaccga accggccatg
    25981 gtggtgatcg attccgttca gacggtttat tccagcgacc tgatgtccgc gccgggaagc
    26041 gtcgggcagg tgcgtgaagc gtcgtcgcgc ctgattctgg ccgccaagaa aagctccatt
    26101 ccggtgtttc tggtcggcca tgtgacaaaa gacggctcga ttgccgggcc gaaagtgctg
    26161 gagcacatgg tggatacggt tttgtatttt gagggcgatt ccgggcacgc ctaccggatt
    26221 gtccgaagca tgaaaaaccg gttcggaccc gccaatgaaa tcggcgtttt tgaaatgcgc
    26281 gaccgcggac ttcaggaagt gcccaacccc tctgcctttt tcctggccga acggccggaa
    26341 aacgctcccg gctcggtggt gctggcgagc ctcgaaggca cccgtccgat tcttgtggag
    26401 atccaggcgc tggtgagcca gtccaatttg ggcatgccca ggcgaacggc catcggggtg
    26461 gatcacaaca gggtgtcgct gctggtggcc gtgctggata aaatctgcgg cctgcatctg
    26521 ggcggatcgg atattttcct gaatgtcgcg ggaggcgtcc gtgtggaaga gccggccgtg
    26581 gatctggccg tcgtctcggc catggcgtcc agttttctgg accggccggt ggacgcccgg
    26641 acggtgattc tgggggaagt cggcctcacg ggcgaggtgc gggccgtctc gcagattgaa
    26701 acgcgggtga aggaagcggc ccgtctgggt tttaaccggt gcatcgtgcc caaaaccaat
    26761 attgcctccc ttggcaaagc ggcaaaaatg gaaatccaaa ccatcacttc cctgaaggaa
    26821 ctgctggata tactcttctg aggacatcgt aagaatttcc ccaaaaatag tgtcccaatg
    26881 ccttgacagg ggttaaatcg tgcataaaat agcgccgcta attcgatagt ttgcaattta
    26941 atatttacaa ggaagaaagg agaagaaacc gtatgaaaat cagaccctta caggatcgga
    27001 tcattgtaaa acgattggaa gaggaaacca aaaccaaagg cggcatcatc attcccgaca
    27061 cggccaagga aaaacccatt gaaggcaaaa taattgcggt cggcaaaggc aaagtggcgg
    27121 atgacggcaa actgatcaaa cccgaagtca gggtgggcga caaagttctt ttcagcaaat
    27181 acggcggaac cgaagtgaaa atcgacggcg aagaatacct gatcatgaga gaagacgaca
    27241 ttctgggcat tattgaaaaa taagaaaata taaggaggaa tctgtacaat gggagcaaaa
    27301 atacttcttt acgatgaaga agcaagaaaa tcggtcttaa agggcgtcaa caccctggcc
    27361 gatgccgtga aagtaaccct cggtcccaaa ggccgcaatg tgattattga caagagcttt
    27421 ggttcaccca ccgtcacgaa agacggcgtc accgtcgcca aagaaatcga actggaagac
    27481 aaatttgaaa acatgggcgc gcagatggtc aaggaagtcg cgagcaagac gagcgacgtc
    27541 gccggcgacg gcaccaccac cgccacgctt ttggctcagg ccatctaccg cgaaggcgtc
    27601 aaggccgtcg cggcaggttc caatcccatg gatgtcaaac gcggcatcga caaggccgtc
    27661 gaagtcgtcg ttaaagagct cggcaagatg agcaaaccca ccaaagatca gaaagaaatc
    27721 gcccaggtcg gcaccatttc cgccaacaac gatgaaacca tcggcagcat catcgccggc
    27781 gcgatggaca aagtcggcaa ggaaggcgtc atcaccgtgg aagaagccaa agggctcgaa
    27841 accgaactgg agatcgtgga aggcatgcag ttcgaccgcg gctacctgtc cccttatttc
    27901 gttacgaatc ccgacaagat gatcgttgcg ctggaagacg cgctcatcct gatctatgaa
    27961 aagaaaatca gcggcatgaa agacctgctg ccgatcctcg agcagatcgc caaaatgggc
    28021 cgcccgctcc tcatcatcgc cgaagacatc gaaggcg
//
LOCUS       DAOPAF010000018        27101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1934_length_27101_cov_63.535088, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000018 DAOPAF010000000
VERSION     DAOPAF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27101
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_27101_cov_63.535088"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLQ77_03360"
     CDS             complement(<1..1285)
                     /locus_tag="PLQ77_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL09749.1"
                     /translation="MKLFKKVLFLSMAMVLCLSAVVMAAQKEQRHGVIVVGAGIAGLS
                     AAWELEQKNIDVAVIEMAPTYGGTGLTSEGALCIIGTPEQEQGGDPDDPRMAYADFMT
                     AGRDEQGPGPNAEWVNYYVKESRREIYDWLTGFGVRFEKKVILMPDNTVPRWHKVVGK
                     GRGLLEPIYRDCGKNGKVKFYYRFKAVSLLRDKSGRIEGVRTLRLKDGAQIDFFAPVV
                     ILATGGFQSNLDMVRKNWPKDLPFPDEILIGGGLNADGSGHAMAKTVGARLDNMHYQL
                     NYPTGLKRPSNATGHRGLNAYTDHSIWVNKTGKRFMNESRDTRKTFPEVVRQPGGTYW
                     AIFDNEARKVFHVSGWSRQLVESELFNNPQYSEVVKSAMTIRELGVAAGLSPETLEET
                     VRRWNEMVTAGRDKDFGRIGTSRSVWAHPPRIEKPP"
     gene            complement(2014..2862)
                     /locus_tag="PLQ77_03365"
     CDS             complement(2014..2862)
                     /locus_tag="PLQ77_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL09750.1"
                     /translation="MTPVSAIIRRRFSCRSYDNKPLDDDLLRTFRQAVEASAPGLFGN
                     APRFVLLEMDELPRDVWKKLGTYGVIQNARLYLAGMIRPAPMATVDYGYCKEKLILKA
                     TELGLGTCWLGGTFAISAFGRAAGMRRDELMPTVSPVGFATGRLRLAERILRGFAGSD
                     HRLPWTELFFRDSTAAPLSAGEAGPYAEALENVRLAPSASNKQPWRIIREPEQSAFSF
                     YLSRSAGYRHLRDVSLQEIDMGIAMCHFDLTVKASGIAGSWHQDDAAPKIKSWEYTAA
                     WQAVGE"
     gene            2966..4537
                     /locus_tag="PLQ77_03370"
     CDS             2966..4537
                     /locus_tag="PLQ77_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPL09751.1"
                     /translation="MKLATVEQMREMDGYAVEKLGMAEEILMENAAHAAVSLLQKTFG
                     IQGRMFVIFCGAGNNGGDGLALARLIKSAGGRVKVFLTASPKKYGGAAKINYDILCNL
                     DADIQLLTKAQESRADVLAADVLVDAIFGTGLDRDVRGLAADVIALVNASGRPVVSLD
                     IPSGISGNSGQVMGEAIRATHTVAFGLPKLGNLLYPGYEHCGALWVTHISFPPSLYES
                     DALTVETNGTATALPPRPAQAYKSSMGDVLFIAGGANYFGAPYFSAMAFLKSGGGYAR
                     LAAPASIIPFVAQSGREIVYLPQRETALYSLSLKAKPDLLALAEKADMVVMGPGLSLQ
                     EETTKLIRELAAVISRPLLIDGDGLTAIAERPEILLKRKAETILTPHAGEMARLTRKT
                     IACVNADKIGVLREASKKLNATVVFKGPHSLIGLTDGRVYVNLSGNPGMATAGSGDVL
                     TGCIAAMHGLGLPPPLATRKGVFLHGCAGDLAAAQKGRDGMTATDILEHLPQALKNDR
                     ESTAAEKQYEPPVLF"
     gene            4655..5065
                     /locus_tag="PLQ77_03375"
     CDS             4655..5065
                     /locus_tag="PLQ77_03375"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HPL09752.1"
                     /translation="MAGIPHLQWSNSISVRVDSIDAQHKKLFEITNRLIDAFEAGSGD
                     FLEVIGELVEYTTIHFHDEQMVMMNAKFPGLAAHTAEHDKFIAKVEDFLQAYSEGHKD
                     AGRTMLVFLKDWLFAHTSGLDMEFAQYLAKKSAK"
     gene            5103..7391
                     /locus_tag="PLQ77_03380"
     CDS             5103..7391
                     /locus_tag="PLQ77_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPL09753.1"
                     /translation="MADASKIAELQDTLRQQKGLQNLTNRINAATGIPQMVAEVRPLL
                     IDLFRVEAAHVYVREKGRKDVHTFIAPAGQTAEVKTALGPQTLPGYVVNTGKMIHVND
                     VGDPGQTEAYQDFPLGEGENRRFGVGVRQILAMPVFYGGETIGAVEVINKKEAAPFGQ
                     KEPALLQNISGLLGVALYNFLLIDPKAKRLKGTYLVSNGLITQEAMDRAKEEARVTKE
                     PLQDVLVKKFNISKEDVGRACEFFWQCKFVSFDKKTPIPGELLKNLKRDYLIREVWVP
                     LEKTEKGVRVIMDDPQDILRRDAIQKSLKIKSVQFDVALKEDILQFIDLFMQSGAEEA
                     SFSDLLGKLETGEGEKKEEPEDVDESDSAIIQLVNKIIGDAFNRRASDIHIEPNVIDK
                     NVLVRFRVDGDCALYQTVPYSYRSALISRIKIMANLDITVKRVPQDGKIKIKRSGADE
                     IELRVVTIPTQGGVEDVVMRILAKGDALPLSAMRMSEKNYQDILKICEQPYGMILVVG
                     PTGSGKTTTLHAILNHINKPDRKIWTAEDPVEITQRGLRQVQVQPKIGFDFAAAMRAF
                     LRADPDVIMVGEMRDFETAKIGVEASLTGHLVLSTLHTNSAPETISRLLDMGIDPLNF
                     ADSLLAVLAQRLVRTLCARCKEAYHPSREEYDQIAQSFGAEYFEKQNLPYSDALTLYR
                     PKGCDECDQTGYKGRIGIHELLLNSPEIRKLAIDRQESIETLRKVAMEQGMLTLYQDG
                     ILKAFQGLTDIKQVHRVCVTSK"
     gene            complement(7438..8412)
                     /locus_tag="PLQ77_03385"
     CDS             complement(7438..8412)
                     /locus_tag="PLQ77_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPL09754.1"
                     /translation="MDLADVFSLYKEEMSRVETHLDRYITSDVRLIPDVAHHLIDSGG
                     KRFRPLLHLICARLCGYDGPRRFPLAASIEFIHTASLLHDDVIDEGLLRRGKTSANRV
                     WGNAASVLVGDFLYSKAFSLLADIGDIRVVQLMSKMTNVMSEGEVFQLMKCGDTSLTE
                     EEYLSIVEKKTAVLISSACESGAALGGAPESQARALGQYGYNIGMAFQITDDTLDYMA
                     REEDFGKSIGKDLEEGKMTLPLIYTLSHCPPDRREKIKSAIEKKDLASEAIRNIFEVI
                     QQSGGIHYSLKRAEQFITQAVNNLDVFSASPEKEHLTAVAGYILSRKT"
     gene            complement(8443..9819)
                     /gene="rimO"
                     /locus_tag="PLQ77_03390"
     CDS             complement(8443..9819)
                     /gene="rimO"
                     /locus_tag="PLQ77_03390"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPL09755.1"
                     /translation="MKKLLAGKLHIISLGCSKNLIDSEVMGALARDSGMTMANRPQDA
                     DVVLVNTCGFIGPAKEESLEAILALAEIKKQRGGAIRLVVAGCLAQRYGRKLLSEIPE
                     ADLWIGTGEVGRIAEHLQGLQAGGAKRKAIVSRPDFLMTAHHKRVLPSGAATAYLKIS
                     DGCSNRCTYCAIPSIRGRARSRRPEDIVLEAENLVARGVREIILIGQDTTAYGKDLKN
                     RPLLSGLLTDVSAVSGLRRLRLLYAHPAHVTTGVLETMASRENICRYLDLPIQHIDDA
                     ILAAMNRRVSSKTIREIIRQARGMMPDLALRTSLIVGFPRETPKRFDRLMDFVRNMRF
                     DHLGVFTYSREEGTPAADLPSRISEKEKERRRDAVMNEQADISLAVNQTLIGSIQEVL
                     IEGKSERRGFALVGRCRRQAPDVDGVTYLKKGRAAAGDFVRCKITGCDHYDLFGEILF
                     DNCRKMKL"
     gene            complement(9816..12059)
                     /locus_tag="PLQ77_03395"
     CDS             complement(9816..12059)
                     /locus_tag="PLQ77_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPL09756.1"
                     /translation="MQEDFSHPPSARFREIKGVVCLALSLFLFLCLFSFSPDDPSPMK
                     FIGDPSSTRNWTGIVGSHAAGWMIFLLGLASFLLPAASLALAFQFFRRPDFGLKIQRV
                     TGFLFLTLACAALFDALIPGGVTVYKTTFPSGGVIGAGLVRFLQGYFNPVGTFILLIV
                     WMMVALFFTVEFSLVSATERFSQSVRIGLSAAWGRIVSFCSGWWTRLKTEKNPPPVIE
                     DAGAKAPPRKAKAKKAEQTHFDFSRMKPDGDFQLPPFTLLSEPPQKDTRVKRDHLITN
                     SRILEKKLADFGVEGRVVEVLPGPVITMYELEPASGVKINRITSLTDDLALALMAPSI
                     RILAPIPGKSVIGIEIPNLKREPVFLKDVLDNDAFTESSLRLPIALGVDFVGAPVIAD
                     LARMPHLLIAGTTGSGKSVALNAMICSILFKAPPQDIKFLMVDPKRLELSSYEGIPHL
                     MHPVVVDPKKASQVLRWTVEEMERRYQIISQMGVKSIDAYNEMLVTRGKTRKAAREPK
                     PEPRPDADTEEAAIESDAPFEHVQLPYIVVVIDELADLMMVAPRDVEESLTRLAQMAR
                     AAGIHLILATQRPSVDVITGLIKANFPTRISFKVSSKIDSRTIIDQPGAEQLLGAGDM
                     LFIPPGTSKLTRIHGAYLSDAEISRIVEFIKMQAQPIYDASIEKFELESGQTRQDEDG
                     YDEKYDEAVALVGELGQASISLVQRYMKIGYNRAARMIEQMEREGIVGPSDGAKPRKV
                     LIRKLPQ"
     gene            complement(12059..12820)
                     /locus_tag="PLQ77_03400"
     CDS             complement(12059..12820)
                     /locus_tag="PLQ77_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL09757.1"
                     /translation="MIRSAVLVSIGVAITAFFSFWAVTYSFFDNAENNVHKVARIWSR
                     ILLAISKTKVQVIGRENIAPGRPQVLMANHQSDFDILIALAFVPGQFRWIAKKELFAV
                     PVFGQAMRSAGYIEIDRQNHEKALRSLDLAALRIREGKSVMTFPEGTRSRDGEIKAFK
                     QGTFYLAIQAGAPIVPISIVGSGKIMPKRSLKIKSGKIKLVIDKPIPAVGYTLESRHE
                     LIQRVRQVIISNYNAYRDAGTASLKDVQVDAKATL"
     gene            13197..14066
                     /locus_tag="PLQ77_03405"
     CDS             13197..14066
                     /locus_tag="PLQ77_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL09758.1"
                     /translation="MELFLQQVFNGIMLGSTYAIVALGLTMVFGILHVPNFAHGQMYM
                     LGAYVCFFLITMFGFSYWPALAVSAVALALGGVILEVLVFKPVRNAGGITPFIAALGA
                     LIILENAVVVLWGPAGHRIPNPYPGIIDAFGITMSTQRLLVIGVAFLLIVLLQIFIKK
                     TTLGSTIEAVAQNKEGATLVGINVNLVSAMTFAISTGLAAIAACLVAPIFMISPSMGS
                     LVGMKAFVIVILGGLGSIPGAIVGGIILGLIEAIGGGYLSAAYKDVFGFGALVLILAI
                     RPTGLFGKEIKGR"
     gene            14063..15058
                     /locus_tag="PLQ77_03410"
     CDS             14063..15058
                     /locus_tag="PLQ77_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL09759.1"
                     /translation="MMKWLKANWFYLAVLAAALVLPLFITNRYYSQVITMSLLFAIGA
                     LSLNLIMGFTGQASLAHGGFFAIGAYGVAILTYTLGWNFWLALPASALLAGAVGFVVG
                     LPALRTRGSYFAITTLCLGEIIYLIAGNWMELTGGHNGIVGIPVPSPIMIPGMGQLNF
                     ESQIPQYYLVLFFLLATLFVLYRLTKSLRGLTFLATAINEDLAEAVGINTFRTKLLSF
                     VVANFFAGLAGGIYASLIGSISPSVASVAMTFNFLMYVLLGGITTLAGPIIGAFLIPV
                     LLEYLQFLQDYQMILFGFLLIIVIIYFPTGFMGLYKKVSDKMKKSKVGAKRAAER"
     gene            15042..15806
                     /locus_tag="PLQ77_03415"
     CDS             15042..15806
                     /locus_tag="PLQ77_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09760.1"
                     /translation="MLLKGENLVKNFGGTIAVNDVSFEVHEGEILSIIGPNGAGKSVL
                     FSLISGAGKPTSGSIRLAGDEIAGQSASQISRKGIARTFQLTALFEKLPVAANLALGY
                     YKRTKSGFWGGILHTPQWKRDKKMVEEKVLQTLEFVGLGPRAFDIASTISQGEQRLLS
                     IGIALISDPRLILFDEPTGGLIQEDTEMITRLIRKINQKGITVCLIEHKMRMIMDLAD
                     RIVVLNYGRKIAEGKPADVAADPQVIEAYLGRKKDA"
     gene            15799..16503
                     /locus_tag="PLQ77_03420"
     CDS             15799..16503
                     /locus_tag="PLQ77_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL09761.1"
                     /translation="MLKLINVTTMIGEHQVIHDVSLQIARGDFVALLGGNGAGKTSLF
                     RTIVGVLPPAKGRIEFKGEEISHIAAHKIVGKGIALCPEGRQLFPQLSVQKNLMLGAY
                     HRKDKKGVEETLEQVYGLFPRLKERYKQMAGTFSGGEQQMLAIGRAIMSKPEMLLLDE
                     PSMGLAPLVVEGIADAITQLNQMGITVFLSEQNAQIALMITHRGYVLENGRLVLEGLS
                     RDLITNEKVKKAYLGT"
     gene            16687..17235
                     /locus_tag="PLQ77_03425"
     CDS             16687..17235
                     /locus_tag="PLQ77_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL09762.1"
                     /translation="MKNRSILFAALMILLLCLYGQGSFAQGQRAKKAAPAKVVRLAGA
                     QAWDCAACHRTQKVLPKNHLDIQEMPYVGCLVCHLSEEGFAGSLKGKMPGSHLHAFRN
                     VQCAGCHGREKKPSAVAKSRCLDCHGSAAAVAGKTQNTKPNNPHDSPHYGTELDCNFC
                     HHQHAGSEDYCAKCHKFNFAAP"
     gene            17273..18562
                     /gene="nrfD"
                     /locus_tag="PLQ77_03430"
     CDS             17273..18562
                     /gene="nrfD"
                     /locus_tag="PLQ77_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPL09763.1"
                     /translation="MGCPENSSWKGCVQDAVRRVFSGGPVYLFFLGLFAVLLVAGIAA
                     GIHSVYIVGSRHAYGSTREIPLAMLIATYTFFVITSTGLCLVSSIGHIFGVERFMPIA
                     KRAVLLSVITIMSGFLVIGLELENPLRLGIYLFLSPNFTSNIWWMGVLYSFEVVLLIL
                     EFIFILLDNHKASVAAGLLGILFGIAAISNLGGVFAMLNGREFWYGPYLPIFFIASAF
                     LMGCAAVIFFTVLAYRVHREPLDRPMADALATVGKLAGVMLAVNLFFTIWRMVTLAVG
                     GTHELLVRDALVSGPFAFNFWALEVGLGMILPFALILWSAGKRIGPIFLASALVIFSS
                     FFVRLDMVVAGQIVPLYWELGVREYAKLNAYSPTGHEILVVLAGVGLTGAAFLLGEKV
                     FQGFREVEDAGSRFAEVLEDAAQELPSAEKGKVDGSP"
     gene            18559..19308
                     /locus_tag="PLQ77_03435"
     CDS             18559..19308
                     /locus_tag="PLQ77_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL09764.1"
                     /translation="MIIKSLDKEKMMKNPEKISEMDRREFLKIGLVITGVFAGGSIVS
                     VASAAEKIFPNGSIAEKYPYRPHYCMVIRVDRCIGCRRCVEGCVAANAVPSYGWRTLV
                     LEREARQAIGQKREFIPVLCNQCNNPPCVRACPVTATYKDPQNGIVMMDNRKCIGCKI
                     CLAACPYDARYYNEERGAVDKCDFCFETRLSRGEKSTACQAACPADVRVFGDLSDSQS
                     RVYRLVHQLQKPVWVLKKDTDAKPNIFYTKG"
     gene            19320..20396
                     /locus_tag="PLQ77_03440"
     CDS             19320..20396
                     /locus_tag="PLQ77_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL09765.1"
                     /translation="MRKRLLLPLALLLIGCGTAAAADQEIPGILYTRPLESVIFRHQD
                     HARKGSACVVCHTGLFEMEALHVQKNKDYNMESLYKGKYCGACHNGKKAFAADTQCAR
                     CHLGSGVVAPPKDAPPFKNSVALGKGEQSVAFHHDIHLKKSTCRSCHPSPFKPGEGTT
                     RIGMADHGGSRFCFGCHSGRNKDAFAWRDCSRCHRKSVPAPRETIHFGKGTKAVPFTH
                     AGHQLKGGCKVCHPRPFAYRKGTAKIDFDDHRNRKACFACHAKKNGSAFYDCNRCHQD
                     KPAAKPGGPDTLKYQTPWKNVYFHHESHAGFSCASCHPTPFAMKKGKTKMVMSDMLRG
                     KTCGVCHNGTKAFSARECARCHKK"
     gene            20421..21812
                     /locus_tag="PLQ77_03445"
     CDS             20421..21812
                     /locus_tag="PLQ77_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPL09766.1"
                     /translation="MTKNKSGIWSFFASVQLAIVLLSLIAFFALVGTLVPQREAAVAF
                     AERISPALFSFLQKMQIFDLYHSVWFILLSGLLAVNLIICSLDRFPLAWRRFRMKHEP
                     ENEDVFRDVPEENTFLANADVQAAADEALSLLRRKYRAVERADGEQGVWLCAQKGRFS
                     LFGVYIVHVSILVLIAGAILGSVFGVEGYVHIPEGETAEAISLRSGGQPLPLPFAVRC
                     DKFTVEFYESGMPKTFQSDLTFLKDGAAVRTAKLLVNHPVEFGGFRFYQASYGAAPGG
                     KATLALAQDGGRRDVMNVAQGFVFDLPGGEGTFQVLRVEENLMKMGPAVKVAVTSRRG
                     EEAVFWVFQQIEPIKKMSPDIFEQVPVFNPALFRPYTFSLLGLEEKYYTGLQVNRDPG
                     TPVVAVSALLLIGGLMLILFSYARAVWIRVAPSENHVLVAIAGRSYKNQPGLQKEMQY
                     LLAELKDHLEKRQ"
     gene            21809..22645
                     /gene="ccsB"
                     /locus_tag="PLQ77_03450"
     CDS             21809..22645
                     /gene="ccsB"
                     /locus_tag="PLQ77_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPL09767.1"
                     /translation="MIHTTILSWVTFLYFAAFVFYLFRMILGREFWGKAATGAALAGL
                     AAQSVALALRWRASYDLGFGHAPLSNFYESLIFFAWTIVLLYLLMERRLKNRSIGVFV
                     LPVAFLIMAYASIAPGMNQRIEPLIPALQSNWLTSHVLTCFMGYAAFTVAFALGVMYF
                     VKKAAGLKPGVFIRLLPSEFQIDELMYSCAALGFIFLTLGIVTGSVWAHYAWGSYWSW
                     DPKETWSLITWLIYAVMLHTRLVRGWRGRRMAVMSMVGFGCMLFTYLGVNLLPSLHSY
                     MS"
     gene            complement(22747..23226)
                     /locus_tag="PLQ77_03455"
     CDS             complement(22747..23226)
                     /locus_tag="PLQ77_03455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPL09768.1"
                     /translation="MAGIQVGDVFGDFSLKSHTEAAVDTAQFAGQKILLSFHPLAWTE
                     VCAKQMQSLEARLDDFRNHNTVPLGISVDSAPCKKAWAKNLGIEKVDLLADFWPHGGL
                     AARLGLFIDKLGFSERANIILDEKRKAVFVKVYPLRELPDIQEMMDFLRITQLPNQR"
     gene            complement(23303..24001)
                     /locus_tag="PLQ77_03460"
     CDS             complement(23303..24001)
                     /locus_tag="PLQ77_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HPL09769.1"
                     /translation="MLQIVQFRYGDNLAYLLYGPTEALAIDGGAWQEILAFLEQNRLM
                     LAYVTNTHRHYDHTPGDDHLLRKTKAGFLDCTAFADGEKILIDGEPVIAYRTPGHSMD
                     SVCFHAGQNLVTGDTLFNATVGNCFTGDLKGFFDSIQRLMALPDDTRIFAGHDYVRDS
                     LAFARHLEPDNPEIERFRQTCDPHFLFSTLAQEKTMNPYLRFNEEPILRLLQKRGLPH
                     ATEWERWQSLMAIE"
     gene            complement(24012..24551)
                     /locus_tag="PLQ77_03465"
     CDS             complement(24012..24551)
                     /locus_tag="PLQ77_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPL09770.1"
                     /translation="MLSRSAAVLVLVDFQGNLAQSMDRKEELFANTVKLIRGFQILNL
                     PVVATEQTPEKLGTTIPLIARELGSGKPIAKETFSCWANPSFRDELESLTRRHVVLAG
                     IESHVCVYQTALDLLRNGYTVHLAADAVSSRKPENRRIGIDAIRSGGAHLTSAEMVLF
                     ELLGSAADSKSRDIFKIVK"
     gene            24658..25437
                     /locus_tag="PLQ77_03470"
     CDS             24658..25437
                     /locus_tag="PLQ77_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPL09771.1"
                     /translation="MTLPANEKILLHEYREGRFAEVELPFSAEYALTLNINGSPYVTM
                     ACSGKDLREHVTGYLITEGIISGADQIARLEVHEKDLAVDVFLMNDRIVAERLEQIKT
                     ISAAGGRTRTRLPGEELVRTRLPGVGAEVILGAMSEFLSYSREHEATHGVHSAALYSL
                     EGGRLIFFDEIGRHNAIDKVIGRAAIQGISLEDKMICSTGRISSEIAFKIIHARAPVL
                     ISRASPTTLSVSLLRRFNILTVIRAVNGRFFVVNGKESIRV"
     gene            complement(25485..25883)
                     /gene="mce"
                     /locus_tag="PLQ77_03475"
     CDS             complement(25485..25883)
                     /gene="mce"
                     /locus_tag="PLQ77_03475"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPL09772.1"
                     /translation="MNVLKIDHIGIAVKNLAESAKLYEMLGIASVGAEEVAEQKVKVC
                     FFPVGDSEIELLESTSPDGPIAKYIEKNGEGIQHMALRVDDLEAALAELKAKGVRLID
                     EKPRYGAGGAKIAFVHPKSTGGILLELSER"
     gene            complement(25984..26973)
                     /gene="meaB"
                     /locus_tag="PLQ77_03480"
     CDS             complement(25984..26973)
                     /gene="meaB"
                     /locus_tag="PLQ77_03480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPL09773.1"
                     /translation="MMEKRDGQAYIQGVLDRDRRMLAKTITLVESSLPSDQELSRQIL
                     DALMPHTGKALRLGITGLPGAGKSTFIESFGTMLTDRGCRVAVLAVDPSSPKTGGSIL
                     ADKTRMEKLSVNNRAFIRPSPSGKTLGGVARKTRETMLVCEAAGFDAVMVETVGVGQS
                     EVAVASMVDFFLVLMIAGGGDELQGLKKGVLELADAVAVNKADGDNVERAKLARRQYE
                     TAFHLLTPPYANWTPPVVTCSSVTMAGLENILNIILDHRDRLTATGELQAKRKEQARE
                     WLKYLVREGVQEWFYESPAAQVLAEGLDGVERGLVTPTSAAAQVLKCLKGQKF"
BASE COUNT         5513 a         7610 c         7984 g         5994 t
ORIGIN      
        1 atggaggctt ttcgattctg gggggatgcg cccagactga tcgtgacgtg ccgatgcgtc
       61 cgaagtcctt gtcgcggcct gccgtcacca tctcgttcca gcggcgcacg gtctcctcca
      121 gggtctcggg agaaagtccc gccgccacgc caagctcgcg gatggtcatg gccgacttga
      181 cgacctcgct gtactgtgga ttgttgaaga gttcgctctc caccagttga cggctccaac
      241 cggagacatg gaataccttg cgggcttcat tatcaaatat cgcccagtag gttccgccgg
      301 gctgccgcac gacttccgga aatgtcttgc gtgtgtcgcg ggactcgttc ataaaacgct
      361 ttcccgtttt gttgacccaa atggaatgat cggtgtaggc attcaatccg cgatggccgg
      421 tcgcgttgga tggtcgtttc agaccggtcg ggtaattcag ctgataatgc atattgtcca
      481 ggcgggctcc gacggtcttt gccattgcgt gtccggaacc atcagcgttc aggcctcctc
      541 cgatgaggat ctcatcggga aagggcagat ccttcggcca gtttttgcgg accatgtcca
      601 aattgctctg gaatcctccc gtggcaagaa tcacgaccgg cgcgaaaaaa tcgatctgtg
      661 cgccgtcttt gagccttagc gtgcggacgc cctcgatgcg gccgcttttg tcccggagca
      721 gggaaaccgc tttaaaccgg taataaaatt tcactttgcc gtttttgccg cagtcgcggt
      781 agatcggttc aagaagccct ctgcccttgc cgacgacctt gtgccagcgc ggcacggtat
      841 tgtcgggcat cagaatcact ttcttttcaa aacggacgcc aaagcctgtc agccagtcgt
      901 aaatttcgcg gcgggactct ttcacatagt agttgaccca ttcggcattg ggaccgggac
      961 cctgctcatc gcggccggcc gtcatgaaat ccgcataggc catgcgggga tcatccggat
     1021 caccgccctg ttcctgctcg ggagtgccga taatacacag agcgccttca ctcgtcagcc
     1081 cggttccgcc gtaagtgggc gccatctcaa tgacggcaac atcgatgttt ttttgctcca
     1141 gttcccacgc agctgaaagc cccgcgatac ccgcgccgac cacaattaca ccatgccgtt
     1201 gttctttttg agccgccatg accacagccg ataaacataa gaccatagcc atggaaagga
     1261 acagtacttt tttgaatagc ttcatctcaa cctctcaaaa atgcgttatt tcagacattg
     1321 ctttccgcaa tcgatccaaa attgatttca gcaattgaaa agggtgtgca gattcgggta
     1381 aaagtgtcat cggaataagc ggatataaat ccgaagagca ggcgccccga aaaacgcgtg
     1441 taacaaaata tcgggtcaaa ttccacactt ttttcccgga tgggaatttt ttgaggttgc
     1501 tgtcgattca gggaatggcg ggtgttgaag aactaagggg aagaactaag ggtcgaatct
     1561 ttactcttga cgtttcagat tgataacgtg gtaatctgtc accattgcga tctttccggc
     1621 ggctggagaa tgttcttcaa ccgctttttg tttgcttgga acggtttgtc tggatgtttt
     1681 atgcggctgt attaccccac ccatagaatg aagcatattt gctgctttga agtggcaatc
     1741 atcactccaa tcccaaatta cgaattgcaa cttgcaggca tcaggtgaaa cacagattca
     1801 ctcctcccat tttaccccga cgtcccaggg tatcttatgc caattgataa tttttatgaa
     1861 cacaaggtga aagataagta cgcagcaacc ggatgaacct taaaaccttt atggaggcaa
     1921 aagaagatga tcactcaaag aagcttcttg aggccattat gggtgtagaa taattggcaa
     1981 taacaaaccg ggaatttcaa aatgaatata ggtctattcc cccactgcct gccaggcggc
     2041 cgtgtattcc catgatttga tctttggcgc ggcatcgtcc tgatgccagc ttcccgcgat
     2101 tcccgatgct ttgacggtca gatcgaagtg gcacatggcg atgcccatgt cgatttcctg
     2161 cagtgaaaca tccctcaggt gacggtatcc cgcactgcgc gacagataga atgaaaaagc
     2221 gctttgctcc ggttcgcgga tgatccgcca gggctgtttg ttggaggccg aaggcgccag
     2281 acggacgttt tccagggctt cagcgtaagg gcccgcctcc ccggcggaaa gcggggcggc
     2341 ggtgctgtcg cgaaagaaaa gttccgtcca tggcaggcga tggtcggagc ccgcgaagcc
     2401 acgcaggatc cgctccgcca gcctcagtcg ccccgtcgcg aagcccacgg gactgacggt
     2461 cggcatcagt tcatcgcgcc gcatgcccgc cgctcggccg aaggcgctga tcgcgaaggt
     2521 gccgcccagc cagcaggtgc ccaggcccag ttcggtggcc ttcaggatga gtttttcctt
     2581 gcagtatcca tagtcaaccg tagccatcgg cgcgggtcgg atcattcccg cgaggtacag
     2641 ccgggcattt tgaatcacgc cgtacgtgcc cagttttttc cagacgtcac gcggcagttc
     2701 gtccatctcg agcaggacga agcgcggcgc gttcccgaac aatcccggcg cggacgcttc
     2761 gacggcctgc cggaacgtcc gaagaaggtc atcgtccaga ggtttattgt cgtaagatcg
     2821 gcaggaaaaa cgcctgcgga tgatcgcgga tactggggtc atatcgcctc ccgaaaatat
     2881 ttattgtcaa atattgcgcg attcctttat actatcaaca aaaaaattga ttcacgactt
     2941 ttccatttgg aggaatagcg ggactatgaa actcgccacg gttgaacaaa tgcgtgaaat
     3001 ggacggctat gccgtcgaaa agctggggat ggcggaagaa atactcatgg aaaacgcagc
     3061 gcacgcggct gtatccctgc tgcaaaagac ttttggcatc cagggcagga tgtttgtcat
     3121 cttctgcggc gccggcaaca acgggggcga cggtctggct ctggcccggc tgattaaatc
     3181 ggccggcggc cgggtaaaag tatttttgac ggcttctccc aaaaaatacg gcggggccgc
     3241 caaaatcaac tatgacattt tatgcaacct ggacgcggac attcagcttc tgacaaaggc
     3301 ccaggagtcg cgcgccgacg tgctggccgc cgacgtcttg gtggatgcga ttttcggcac
     3361 ggggctggac cgcgacgtca gggggctggc ggccgatgtg attgcgctcg tcaacgcctc
     3421 cggccggccg gtggtgagtc tggacattcc ctccggcatc tccggcaaca gcgggcaggt
     3481 gatgggtgag gcaattcgcg cgacgcacac ggtggcgttc ggtttgccga agctcgggaa
     3541 ccttttgtac cccgggtatg aacattgcgg agcgctttgg gtgacgcaca tttccttccc
     3601 gccttcgctc tatgagagcg acgcgctgac ggtggaaacc aacggtacgg cgacggctct
     3661 ccctccgcgt cccgcccagg cctataaaag ctccatgggc gacgttctgt tcatcgccgg
     3721 aggggccaac tacttcggcg cgccgtattt ttccgcgatg gcttttctga aatcgggcgg
     3781 aggctacgcg aggctcgcgg cgcccgcttc aatcattccc tttgtggccc agtccggccg
     3841 cgaaatcgtt tacctgccgc agcgcgaaac ggcgctgtac agcctctcgc tcaaagccaa
     3901 accggacctg ctggcgcttg cggaaaaagc cgacatggtg gtcatgggcc ccggcctttc
     3961 gctgcaggag gagacgacca agctcatccg ggaactcgcg gccgtcatct cccgtccgct
     4021 tttgatcgac ggggacggac tgacggccat cgccgaacgg ccggaaattc tgctcaaacg
     4081 gaaggcggaa acgattctga cgccgcacgc gggcgagatg gcgcggctga cgcgaaaaac
     4141 catcgcctgc gtgaatgccg acaagatcgg tgtcctgagg gaggcgtcga aaaaactgaa
     4201 cgccacggtg gttttcaaag gcccgcattc cctcatcggc ctgacggacg gccgggtgta
     4261 tgtcaatttg agcggcaatc cgggcatggc caccgccgga agcggcgacg tcctgaccgg
     4321 ctgcatcgcg gccatgcacg gcctggggct gccgcccccg cttgccaccc gcaagggggt
     4381 tttcctgcac ggctgcgccg gagatctggc cgccgcccaa aaaggccggg acggcatgac
     4441 ggcgacggat attctggagc acctgccgca ggcgctgaaa aacgaccgcg aaagcacggc
     4501 ggccgaaaag caatacgagc cgcccgtcct gttctagaga gaggcggctc ccggacggta
     4561 tggcttgctt cagggggctc acttcgggat tgactttccc ccggagccgc gcctatactc
     4621 ccgttaatac aatacgaaag acgggagaaa tgacatggcc ggaatacccc atctccagtg
     4681 gtccaacagc atcagcgttc gcgtggacag catcgacgcc cagcacaaaa aactttttga
     4741 aatcaccaat cgcctgatcg atgcttttga agccggatcc ggcgattttt tggaggtgat
     4801 cggcgaactg gtggaataca cgaccattca ctttcacgac gagcagatgg tgatgatgaa
     4861 tgcgaagttt ccgggcctcg cggcgcatac cgcggagcat gacaaattca tcgcgaaagt
     4921 cgaagatttt cttcaggctt acagcgaagg tcacaaggac gcggggcgga cgatgctcgt
     4981 ttttttgaaa gactggcttt tcgcccacac ctcgggcctg gacatggaat ttgctcagta
     5041 tttggcaaaa aaatccgcga agtgaaatcc gatcgcctca aacgaacaag aaaggtttga
     5101 ccatggcaga cgcgtccaaa attgccgaat tgcaggatac tttgaggcag cagaagggat
     5161 tgcagaacct caccaaccgc atcaatgccg cgaccggcat tccacagatg gttgccgagg
     5221 ttcgtccgct gctgatcgat ctgttccggg ttgaggccgc gcatgtgtat gttcgggaaa
     5281 aaggccgcaa agacgtacat acctttatag cccccgccgg tcagaccgct gaagtgaaaa
     5341 cggctctcgg cccccagacc cttcccggat acgtcgtgaa tacggggaaa atgattcatg
     5401 tcaacgacgt tggcgatccc ggccagaccg aggcctacca ggactttccg ctcggcgaag
     5461 gcgaaaaccg ccgtttcggc gtcggcgtgc ggcagatcct ggccatgccg gttttttacg
     5521 gcggcgaaac gattggcgcg gtcgaggtga tcaacaaaaa ggaagccgcg ccgttcgggc
     5581 aaaaagagcc ggcgcttttg cagaatattt ccggactgct cggcgtggcc ctctacaatt
     5641 ttctgctgat cgatcccaag gcaaaaagat tgaaaggcac ctatctggtt tccaacggcc
     5701 tgatcacgca ggaagccatg gaccgggcca aggaagaagc ccgggtgacc aaggagccgc
     5761 tccaggacgt gctggtgaag aaattcaaca tcagcaaaga agacgtcgga cgggcctgcg
     5821 aatttttctg gcagtgcaaa tttgtttctt tcgacaagaa aacgccgatt cccggcgaac
     5881 tgctgaaaaa tctgaaaaga gactacctga tccgggaagt ctgggtcccc ctggaaaaaa
     5941 cggaaaaagg cgttcgcgtg attatggatg atccgcagga cattctccgg cgcgacgcca
     6001 ttcaaaagtc cctcaagata aaatccgtgc agtttgacgt ggcgctgaaa gaggatattc
     6061 tccagttcat tgacctgttc atgcagagcg gcgcggaaga agcgtccttt tcggatcttt
     6121 tgggaaagct tgagacgggg gaaggcgaga agaaagaaga gccggaagat gtggacgagt
     6181 ccgacagcgc catcattcaa ctggtcaaca agatcatcgg cgacgcgttc aaccgccggg
     6241 cgtcggacat tcatatcgaa ccgaatgtga tcgacaagaa cgtgctggtg cggttccggg
     6301 tggacgggga ctgcgcgctg tatcagaccg tcccttactc ctatcggtcg gcgctgattt
     6361 cccgcattaa aatcatggcc aatctggaca tcaccgtgaa gcgggttccg caggacggaa
     6421 agatcaagat caagcggtcc ggcgcggatg aaatcgagct gcgcgtcgtg acgattccga
     6481 cgcagggcgg cgtggaggat gtggtgatgc gcatcctggc caaaggcgac gcgctgccgc
     6541 tttccgccat gcggatgtcg gagaagaact atcaggatat tttgaaaatt tgcgagcagc
     6601 cctacgggat gattctggtg gtcggaccca ccggctcggg aaaaacgacc acgctgcacg
     6661 cgattttgaa ccacatcaac aagccggacc ggaaaatctg gaccgccgaa gatcccgtgg
     6721 aaatcaccca gcggggcttg cggcaggtgc aggtgcagcc gaaaatcgga tttgattttg
     6781 cggcggcgat gcgggcgttt cttcgcgccg acccggatgt gatcatggtc ggcgaaatgc
     6841 gggattttga gacggccaag atcggcgtcg aggcgtcgct gacggggcat ctggtgttga
     6901 gcacgctgca taccaacagc gccccggaga cgatttcccg gcttctggac atggggatcg
     6961 acccgctcaa tttcgcggat tccctgctgg cggtgctggc gcagcggctg gtccggaccc
     7021 tgtgcgcccg gtgcaaggag gcttaccatc cgagccggga ggaatatgat cagatcgcgc
     7081 aaagcttcgg cgccgagtat tttgaaaagc agaaccttcc ctattccgac gccctgacgc
     7141 tttaccgccc caaagggtgc gacgagtgcg accagacggg atacaaggga cggatcggca
     7201 ttcacgaact gctcctgaat tcaccggaaa taaggaaact ggccatcgac cggcaagaga
     7261 gcattgaaac gctccggaaa gtcgccatgg aacaggggat gctgacgctt tatcaggatg
     7321 gcatattgaa ggcgtttcag ggcctgacgg atatcaaaca ggtgcatcgg gtctgcgtga
     7381 cgtcgaaata gcgccttgtt gcggtttccg tcttttccgc cgccgtcggc atgagcgtca
     7441 ggtttttctc gacaggatgt agccggccac ggccgtcagg tgctcttttt cgggactggc
     7501 ggaaaaaacg tcaaggttgt tcaccgcctg cgtgatgaat tgctccgcgc gcttcaacga
     7561 ataatgaatg ccgccggatt gttgaatcac ctcaaaaata ttccggatgg cttcggaagc
     7621 taaatctttt ttctcaatcg cggatttgat cttttcgcgc cggtccggcg ggcagtggga
     7681 cagcgtgtaa atgagcggca gcgtcatttt tccttcttcc aggtccttgc cgatggattt
     7741 gccgaaatcc tcttcgcggg ccatgtaatc cagcgtatcg tccgtgattt gaaacgccat
     7801 gccgatgttg tagccgtatt gcccgagggc gcgggcctgc gactccggcg cgccgcccag
     7861 cgcggcgccg ctttcacagg cggaggagat cagcacggcg gttttcttct ccacgatgct
     7921 cagatactcc tcttcggtga ggctggtgtc tccgcacttc atcaactgga acacttcgcc
     7981 ttccgacatg acgttggtca tcttcgacat gagctggacg acccggatgt cgccgatgtc
     8041 ggccagaagg ctgaaagctt tggaatacag aaaatcgccg accagaacgc tggcggcgtt
     8101 gccccagacg cggttggccg aggttttccc ccggcggagg aggccctcat cgatgacgtc
     8161 gtcgtgcagg aggctggccg tgtgaatgaa ttcaatggag gccgccagcg gaaagcggcg
     8221 ggggccgtca tagccgcaaa gcctcgcgca gatcagatgc agaagcggcc ggaatcgttt
     8281 gccgccgctg tcgatcaggt gatgcgcgac gtcgggaatc agccggacgt cggatgtaat
     8341 gtaccggtcc aggtgggttt ccaccctgct catctcttct ttgtaaaggg aaaaaacatc
     8401 cgccagatcc atgatcctgc tccgggttcg ttcgtgaaaa gactatagtt tcatttttcg
     8461 gcaattgtca aacaggattt ctccgaaaag gtcgtaatgg tcgcagccgg tgattttgca
     8521 gcggacgaaa tcgccggcgg cggcccggcc ttttttcagg taggtgaccc cgtcgacgtc
     8581 cggcgcctgc cggcggcagc gcccgaccag cgcgaatccc cggcgctcgc ttttcccttc
     8641 gatcagcacc tcctggatgg agccgatcag cgtttggttc acggccaagg aaatgtccgc
     8701 ctgttcgttc atgaccgcgt ctcgccttcg ctccttctct ttttcagaga tgcgcgacgg
     8761 aaggtccgcg gcgggcgtgc cttcttcccg cgaataggtg aaaacgccga ggtggtcgaa
     8821 ccgcatattc cgcacaaaat ccatcaggcg gtcgaaccgc tttggcgttt cccgcggaaa
     8881 acccacgatg agggaggttc gaagcgcaag gtcgggcatc atgcctctcg cctgacgaat
     8941 gatttcccga atggtctttg aagaaacgcg gcggttcatg gcggcaagga ttgcgtcgtc
     9001 gatatgctga atgggcagat cgaggtagcg gcagatgttt tcccgcgagg ccatggtttc
     9061 cagaacgccg gtcgtgacgt gcgccggatg cgcatacaga agccgcagcc ggcgcaggcc
     9121 ggaaaccgcc gacacatcgg tcagcaggcc ggagagaagc ggccggtttt ttaaatcttt
     9181 tccgtaagcg gtggtgtcct ggccgatcag aatgatttcc cgaacgccgc gggcgaccag
     9241 gttttccgct tcgagcacaa tgtcttccgg cctgcggctt cgcgcccgcc cgcggatgga
     9301 gggaatggcg cagtaggtgc agcggttgga gcagccgtcg gatattttca aataagcggt
     9361 ggcggcgccg gaaggcagga cccgtttgtg gtgagccgtc atcagaaaat ccggcctgga
     9421 aacaatcgcc ttccgtttgg ccccgcccgc ctgcagaccc tgcagatgtt cggcgatgcg
     9481 tccgacttct cccgtgccga tccacaggtc ggcttcggga atttcggaaa gcagctttcg
     9541 gccgtagcgc tgggccagac agccggccac caccagcctg atggcgccgc cgcgctgctt
     9601 tttgatttcc gccagcgcca gaatcgcttc cagagactct tccttggccg gcccgataaa
     9661 gccgcaggtg ttgaccagaa cgacatcagc gtcctgggga cgattggcca tcgtcattcc
     9721 ggagtccctg gccagagccc ccatgacttc cgaatcgatc aggttcttgg agcagcccaa
     9781 agagatgatg tgcagttttc cggcaagcaa ttttttcact gcggaagttt ccgaatcaga
     9841 accttgcggg gtttcgcgcc gtcggagggg ccgacgatgc cttcacgctc catctgctca
     9901 atcatgcggg cggcgcggtt gtagccgatt ttcatgtagc gctgcaccag cgagatggag
     9961 gcctgcccca gttcgccgac gagggccacg gcctcgtcgt atttttcatc gtagccgtct
    10021 tcatcctgcc gggtttggcc ggattccagt tcaaattttt caatcgaggc gtcgtagatc
    10081 ggctgggcct gcattttgat gaattcgacg atgcgcgata tctccgcgtc cgacagatac
    10141 gcgccgtgaa tgcgggtgag cttggaggtg ccgggcggga tgaagagcat atcgcccgcg
    10201 cccaggagct gctccgctcc cggctgatcg atgatggtgc gcgaatcgat tttggaggag
    10261 actttgaacg aaatgcgcgt cgggaaattg gccttgatca gtccggtgat gacgtccacc
    10321 gaggggcgct gcgtggcgag gatcaggtgg atgccggccg cccgcgccat ctgcgccagc
    10381 ctcgtcagcg attcttccac gtcccggggc gcgaccatca tcagatcggc cagctcgtcg
    10441 atcacgacga cgatgtaggg cagctggaca tgctcaaacg gagcgtcgct ttcgattgcg
    10501 gcctcctccg tgtccgcgtc gggccgtggt tcgggtttcg gctccctggc cgccttcctg
    10561 gttttgccgc gggtgacgag catttcattg taggcgtcga tgcttttgac gcccatctgg
    10621 ctgatgatct gatagcggcg ctccatttcc tccaccgtcc agcgcagcac ctgcgaggcc
    10681 tttttcgggt ccacgacgac cgggtgcatc agatgcggaa tgccttcgta ggaagacagc
    10741 tccagccgct tgggatcgac catcagaaac ttgatgtctt ggggcggcgc cttgaaaaga
    10801 atgctgcaga tcatggcgtt gagcgcgacg cttttgccgg agccggtggt cccggcaatg
    10861 agcaggtgcg gcattctggc caggtcggca ataaccggcg cgccgacgaa atccacgccc
    10921 agcgcgatgg gcagacgcag ggaggattcg gtaaaggcgt cgttgtccag cacgtccttg
    10981 aggaacaccg gctctctttt gagattggga atttcaatgc cgatcacgga cttgccggga
    11041 atcggcgcca gaatgcggat gctgggcgcc atgagcgcca gcgcaaggtc gtcggtcagg
    11101 ctggtgatgc ggttgatctt gacgcccgaa gccggctcca gctcgtacat ggtgatgacc
    11161 gggccgggca gaacctccac cacgcgccct tcgacgccga agtcggccag cttcttttcc
    11221 agaatgcgcg agttggtgat caggtggtcg cgtttgacgc gggtgtcttt ttgcggcggc
    11281 tcgctgagca gcgtaaacgg cggcagctga aagtcgccgt cgggcttcat tcgggaaaaa
    11341 tcaaaatgcg tctgctcggc ttttttcgct tttgcttttc tgggcggggc ttttgccccg
    11401 gcgtcctcga tgacgggcgg cggattcttt tcggttttca gccgcgtcca ccagccgcta
    11461 cagaaggaaa cgatccggcc ccatgcggcg gaaagcccga tccttacgga ctgcgagaat
    11521 ctttcggttg ccgacaccag cgagaattcg acggtgaaaa acagcgcgac catcatccag
    11581 acgatcagaa gaataaaggt tcccaccgga ttgaagtagc cctgcagaaa ccggacaagg
    11641 ccggcgccga taacgcctcc cgacgggaag gttgttttgt acaccgtgac gccgcctggg
    11701 atcagggcgt cgaaaagagc ggcgcaggca agcgtcagaa acaggaagcc tgttacgcgc
    11761 tggattttca ggccgaaatc cggacgccgg aaaaattgaa atgccagcgc cagggaagcg
    11821 gcgggcagca ggaaagaggc aaggcccaga agaaaaatca tccagccggc cgcatgggag
    11881 ccgacgatgc ccgtccagtt tctcgttgac gaggggtcgc cgatgaattt catgggggag
    11941 gggtcgtccg gcgaaaagga aaacaggcac aggaagagaa acagcgacag cgcgaggcag
    12001 acgacgccct tgatttcccg aaatcttgca gacggaggat ggctgaaatc ttcctgcatc
    12061 agagcgtcgc tttcgcgtcc acctggacgt ctttgaggct ggccgtaccg gcatcgcggt
    12121 aggcgttgta attgctgata atgacctgcc ggaccctttg aatcagttca tgccggcttt
    12181 ccagcgtgta tccgaccgcg ggaatcggct tgtcgatgac cagcttgatt ttcccggatt
    12241 tgattttcag cgaacgcttc ggcatgattt tgccgctccc gacgatgctg atcggaacga
    12301 tcggcgcgcc cgcctgaatg gccaggtaaa aagtgccctg cttgaaggcc ttgatttccc
    12361 cgtcgcggct tctcgtgcct tcggggaagg tcatgaccga cttgccttcg cggatccgca
    12421 gggccgccag gtccaggctc cgcagggctt tttcgtgatt ctgccggtcg atttcaatgt
    12481 agcccgcgct tctcatggcc tggccgaaga cgggaacggc aaacagctct tttttggcga
    12541 tccagcgaaa ctgtcccgga acgaaggcca gcgcgatgag aatgtcaaaa tcgctctggt
    12601 ggttggccat cagcacctgc ggtctgccgg gcgcgatgtt ctcccggccg atgacctgca
    12661 ctttcgtttt gctgatcgcc agcaaaatcc ggctccagat ccgggccacc ttgtggacgt
    12721 tgttttccgc attgtcaaaa aaggaatagg tcaccgccca gaatgaaaag aaagcggtga
    12781 tggccacgcc tatggagact aaaacggctg aacggatcat cggtattttt tcctgctcga
    12841 atgccgaggc gccaagaatg ttttttcaag gtttttctac ttgataaacc tgttgaagtc
    12901 aacagtgaat cgcgtttttc tcccgaggca tccgccctcg ttcggacgga tcatttgcgg
    12961 cctgccgatg actcccggtc acgtcgtgtt tcaatcagga taagggattg cggcttttga
    13021 taaatttgtc aaaaatgccg aaaaaaagga ttttccgctt gacaaatggt tgccgctcat
    13081 ttaagaagcc tgcctaagca aaagacattc aagagcaaca ccaatggaac gccgcccgga
    13141 ctttatgtgt tgaaaggcag ggaacggggc tgcgtcattc ggataatgga gtatccatgg
    13201 aactatttct gcagcaggtt tttaacggaa tcatgttggg gagcacgtat gccatcgtcg
    13261 ccctgggcct gacgatggtt ttcggcattc tgcatgtgcc gaactttgcg cacggccaga
    13321 tgtacatgct gggcgcctat gtctgcttct ttttaattac catgttcggt ttttcctact
    13381 ggcccgcgct ggcggtgtcg gccgtcgcgc ttgcactggg gggcgtcatt ctggaggtcc
    13441 tggtcttcaa gccggtccgc aacgccgggg gcatcacccc gttcattgcc gccctgggcg
    13501 cgctgatcat cctcgaaaac gcggtcgtcg tgctttgggg accggcaggc caccggattc
    13561 cgaatccgta tcccggcatc atcgacgcct tcggcatcac gatgtccacg cagcgcctgc
    13621 tcgtgatcgg agttgccttc ctgctgattg ttctcctgca gatcttcatt aaaaaaacaa
    13681 cgctgggcag caccatcgag gcggttgccc agaacaagga aggcgccacg ctggtcggca
    13741 tcaacgtcaa cctggtatcc gccatgactt ttgccatctc caccgggctg gccgccatcg
    13801 ccgcctgcct ggtcgccccc attttcatga tttctcccag catgggctcg ctcgtcggca
    13861 tgaaagcctt tgtcatcgtt attctcggcg gactgggaag cattccggga gcgatcgtgg
    13921 gaggcatcat tctgggattg atcgaggcca tcggcggcgg gtatttgtcg gcggcataca
    13981 aggatgtgtt cggtttcggc gctctcgtgc tgatcctcgc gatcaggccg accggcctgt
    14041 tcgggaagga gattaagggc agatgatgaa gtggctgaag gcaaattggt tttatctggc
    14101 ggttctggcg gcagccctgg ttcttccgct tttcataacg aaccggtatt acagtcaggt
    14161 cattacgatg agcctgcttt ttgcgatcgg cgcattgagc ctgaatctga ttatgggctt
    14221 taccggacag gcctcgctgg cgcacggcgg gttttttgcg atcggcgcat acggcgtcgc
    14281 gattctcact tatacgctgg gctggaactt ctggctggcc cttcccgcgt cggcgcttct
    14341 ggccggtgcg gtcggatttg tggtcggtct gccggcgctg cgcacccggg ggtcctactt
    14401 cgcgatcacc acgctgtgcc tgggcgaaat catctacctg atcgcaggca actggatgga
    14461 actgacggga ggccacaacg gcatcgtggg cattcccgtt cccagcccga tcatgattcc
    14521 cggcatgggg cagctgaatt ttgaatccca gattccccag tattacctcg tcctgttttt
    14581 cctgctggcg accctttttg tcctgtatcg cctgacgaaa tccctgcgcg gcttgacgtt
    14641 tctggctacg gccatcaacg aggacctggc ggaagccgtg gggatcaaca ccttccgcac
    14701 caagctgctg tctttcgtcg tggccaattt cttcgcgggg ctcgcgggcg gcatttacgc
    14761 cagcctcatc ggcagcattt ctccctcggt cgcttcagtc gccatgacgt tcaacttcct
    14821 gatgtatgtg ctcctcggcg gcatcacgac actggccggt ccgatcatcg gcgccttttt
    14881 gattccggtt ctcctggaat atctgcagtt cctgcaggat tatcagatga ttttgttcgg
    14941 atttttgctg atcatcgtga tcatctactt cccgacggga tttatgggat tgtataaaaa
    15001 agtgagcgac aaaatgaaga aatcgaaagt gggggccaaa cgtgctgctg aaaggtgaaa
    15061 atctggtaaa aaattttggc ggcaccattg ccgtcaatga tgtcagcttt gaagtccatg
    15121 aaggggaaat cctgtccatc atcggcccca acggcgcggg caaatccgtt ctcttcagcc
    15181 tcatcagcgg cgcgggcaag ccgacatcgg gcagcatccg gttggcgggg gatgaaatcg
    15241 caggccagag cgccagtcag atatcccgca aaggcatcgc ccggaccttc cagttgacgg
    15301 cccttttcga aaagcttccc gtcgcggcca atctggcgct ggggtattac aaacgcacga
    15361 aaagcgggtt ctggggcgga attttgcaca cgccgcaatg gaagcgggac aaaaagatgg
    15421 tggaggaaaa ggttcttcag acccttgaat tcgtcggcct gggccccaga gccttcgaca
    15481 tcgcctccac gatttcccag ggggagcagc ggctgctctc catcggcatc gcgctcatca
    15541 gcgacccccg cctgattctg tttgacgaac cgacgggcgg tctgattcag gaagacacgg
    15601 agatgatcac gcggctgatc cgcaagatca accagaaggg catcaccgtt tgcctgattg
    15661 aacacaagat gcggatgatc atggatttgg cggacaggat tgtcgtgtta aactacggcc
    15721 ggaaaattgc ggaaggcaag ccggcggacg tggccgcgga cccgcaggtc atcgaagcct
    15781 acttaggaag aaaaaaggat gcttaagtta ataaatgtca cgacgatgat tggcgagcat
    15841 caggtgatcc acgatgtttc gctgcagatt gcccgcggag actttgttgc gctgttgggc
    15901 ggaaacggcg ccgggaaaac cagtttgttc cgcaccattg tcggagtttt gccgccggca
    15961 aaaggcagaa tcgaattcaa aggcgaggag atcagccaca tcgccgcgca taaaatcgtg
    16021 ggaaagggca tcgcgctctg tcccgagggg cgccagctct ttccgcagct ttccgtccag
    16081 aaaaatctca tgctgggcgc gtaccaccgc aaggacaaga agggcgttga agagacgctg
    16141 gagcaggttt acgggctttt cccgcgcctc aaggagcgct acaaacaaat ggccggaact
    16201 ttttccggcg gcgagcagca gatgctggcc atcgggcggg ccatcatgtc caagccggag
    16261 atgctccttc tggacgagcc ctccatggga ctggcgccgc tggtggtgga aggcatcgcc
    16321 gacgcgatca cgcagctcaa tcaaatgggc atcaccgtct ttttgtccga gcagaacgcg
    16381 cagatagccc tgatgatcac gcaccggggc tatgttctgg aaaacggccg tctcgtgctg
    16441 gagggattga gcagggacct gatcaccaat gaaaaggtga aaaaggcgta cctgggaacc
    16501 tgaatttttc cagccgccgg atcggactca aatcctgaat tccaagagct cttgcccctt
    16561 cggggggaga gctcttttta tcggtgtctc cgaaaggttg cgccggttgc cgcagagcat
    16621 gaattggttt gacacgaacc ggcttatttg ttagaaatga cccagcttgc gagcttgcga
    16681 ggttttgtga agaaccgatc aatattgttt gccgctctga tgattctcct tctttgcctg
    16741 tacggacagg gaagctttgc ccagggacag cgggcgaaga aagcagctcc agccaaggtt
    16801 gtccggcttg ccggcgcgca ggcctgggac tgcgcggcct gccatcgcac gcaaaaagtt
    16861 cttccgaaaa atcacctgga cattcaagaa atgccgtatg tcggctgcct ggtttgccat
    16921 ctttccgaag aaggctttgc cggctcgctc aaggggaaaa tgcccggcag tcacctgcat
    16981 gccttccgga atgttcaatg cgccgggtgc cacggccggg agaaaaagcc gtctgcggtc
    17041 gccaaaagcc ggtgtctgga ctgccacggc agcgctgccg cggttgccgg aaagacacaa
    17101 aacaccaaac cgaacaatcc gcacgactcg ccccactacg ggacggagct ggactgtaat
    17161 ttttgccacc accagcatgc cgggtcggag gattattgcg cgaagtgcca caaatttaat
    17221 tttgcggcgc cgtaattcca accggcccgg gaaggcataa ggaaaaatga cgatgggatg
    17281 tccggaaaat tcatcatgga aaggctgtgt tcaggacgca gtccggcgcg tcttctccgg
    17341 cggcccggtg tatctgtttt ttctgggtct gtttgccgtg ctgctggtcg cggggattgc
    17401 cgcgggaatt cactccgtct acatcgtggg aagccgccac gcctacggca gcacccggga
    17461 gattccgctg gccatgctca tcgccaccta tacctttttt gtcatcacct cgaccggact
    17521 gtgcctggtg tcctccatcg gtcacatctt cggcgtggag cgtttcatgc ccatcgcgaa
    17581 gcgggcggtg ctgctgtccg tgatcaccat catgtcggga ttcctcgtca tcggcctgga
    17641 gctggaaaat ccgctgcggc tgggcatcta cctgtttctc tcgccgaact tcacctccaa
    17701 tatctggtgg atgggggttc tctactcctt tgaggtggtc ctgctgatcc ttgaattcat
    17761 cttcatcctg ctggacaacc acaaagcgtc ggtggcggcg ggccttctgg ggatcctctt
    17821 cggcattgcc gccatcagca acctgggggg cgtctttgcc atgctcaacg gccgggaatt
    17881 ctggtacgga ccctatctgc ctattttctt cattgcctcc gccttcctga tgggatgcgc
    17941 cgccgtgatt tttttcacgg tcctcgccta ccgcgtccat cgcgaaccgc tggaccgccc
    18001 gatggccgat gctctggcga cggttggcaa gcttgccggc gtgatgctgg cggtgaacct
    18061 gtttttcacg atctggcgga tggttacgct ggcggtggga ggaacgcatg agctgctggt
    18121 gcgcgacgcc ctggtcagcg gtcctttcgc cttcaatttc tgggcgctgg aggtcgggct
    18181 gggcatgatt cttcccttcg cgctgattct gtggtccgcg ggaaagcgga tcgggccgat
    18241 ttttctggct tctgccctcg tgattttcag ctcttttttt gtgcgcctgg acatggtggt
    18301 tgcgggacag attgtccccc tgtactggga gctcggcgtc agggagtatg ccaaactgaa
    18361 cgcgtattcg cccaccgggc atgaaattct ggtggtgctg gccggcgtcg gccttaccgg
    18421 cgcggcattt ttgctgggag aaaaagtttt tcagggattc agagaagttg aagacgccgg
    18481 atcccgtttt gccgaagtgc tggaagacgc cgcgcaggaa ctcccatcgg cagaaaaggg
    18541 aaaggttgac ggctcgccat gatcatcaaa tcgctggaca aggaaaagat gatgaaaaac
    18601 ccggaaaaga tttccgaaat ggaccgccgg gaatttctga aaatcggctt ggtcattacc
    18661 ggtgttttcg cggggggctc catcgtatcg gtggcgtccg cggccgaaaa aatattcccg
    18721 aacggatcga tcgcggagaa atatccctac cggccgcatt actgcatggt gatccgcgtg
    18781 gaccggtgca tcggctgccg ccgctgtgtc gaggggtgtg ttgccgccaa cgccgtgccg
    18841 tcctacggct ggcgcacgct ggtgctggaa agggaagccc ggcaggccat cggccaaaag
    18901 cgggaattca ttcccgtcct gtgcaatcag tgcaacaatc ctccctgtgt ccgcgcctgt
    18961 cccgtaacgg cgacgtacaa ggatccgcaa aacggcatcg tcatgatgga caaccggaaa
    19021 tgcatcggct gcaaaatctg tttggcggcc tgcccgtacg acgcccgcta ctacaatgag
    19081 gaaagaggcg ccgtggacaa gtgcgatttt tgctttgaaa ccaggctgtc gcgcggcgag
    19141 aaaagcacgg cctgccaggc ggcgtgcccg gcggatgtcc gggtgttcgg ggacctgtcc
    19201 gattcccaaa gccgtgttta ccggttggtg catcagttgc agaagccggt ctgggtgctt
    19261 aaaaaagata cagacgccaa acccaatatt ttttacacga aagggtaggc aggatcagca
    19321 tgcgaaaacg tctccttctt ccattggctc tgcttttgat cggttgcgga actgccgcgg
    19381 cggcggatca ggaaatcccc ggcattcttt acacccggcc gctggaatcg gtcatcttcc
    19441 gccatcagga ccacgcgcgg aagggaagcg cgtgcgtcgt ctgtcacacc ggcctgttcg
    19501 aaatggaggc cctccacgtc cagaaaaaca aagattacaa catggagtcc ctgtacaagg
    19561 ggaaatactg cggcgcctgt cacaacggca aaaaggcttt tgccgcggac acgcagtgtg
    19621 cgcgctgcca cctcggatcc ggcgttgtgg cgccgcccaa agatgcgccg ccgtttaaaa
    19681 attccgttgc tctgggaaaa ggcgaacaaa gcgtggcgtt ccaccatgac attcacctga
    19741 aaaaatcgac ctgtcggagc tgtcatccgt ccccgttcaa gcccggcgaa ggaaccaccc
    19801 ggatcggcat ggcggaccac ggcggcagcc ggttttgttt tggctgccac agcggcagga
    19861 acaaagacgc ctttgcctgg agggattgca gccgctgcca tcggaaatcc gttcccgctc
    19921 cccgggaaac catccatttc ggcaagggga caaaagccgt cccgttcacg catgccggcc
    19981 accaactgaa aggcggatgc aaggtctgtc atccccggcc gtttgcctac cggaaaggga
    20041 ccgcaaaaat tgattttgac gatcaccgga accggaaggc gtgttttgcc tgccatgcca
    20101 aaaagaacgg atcggccttt tacgactgca accgctgcca tcaggataaa cctgccgcca
    20161 aacccggcgg gcccgatacc ctgaaatatc aaacgccctg gaaaaacgtg tacttccatc
    20221 acgaaagcca tgctgggttt tcctgcgcct cctgccatcc cactccgttt gccatgaaaa
    20281 agggcaaaac aaaaatggtg atgtcggata tgcttcgcgg aaaaacctgc ggcgtctgtc
    20341 acaacgggac caaggccttt tccgcccggg aatgcgcccg gtgccataag aaataaatcc
    20401 aacagcggga gaaggcgctt ttgaccaaaa acaaatccgg catctggtcg ttctttgcat
    20461 ccgttcaact ggccatcgtg ctgctttccc tgatcgcttt ttttgccctg gtcggcacgc
    20521 tggtccccca gcgggaggcg gcggttgcct ttgccgaacg catttcgccc gcgctttttt
    20581 cctttcttca gaaaatgcag atcttcgatc tgtatcactc cgtgtggttc atccttttat
    20641 cgggtcttct ggccgtcaac ctcatcatct gttcgctgga tcgctttccc ctggcgtggc
    20701 gccgcttccg aatgaagcat gagcctgaaa atgaagacgt ctttcgggat gtgccggaag
    20761 aaaatacgtt tctcgcgaac gcggacgttc aggcggcggc ggacgaagcc ctgtccctgc
    20821 tgcgaagaaa atatcgcgcc gtggaaaggg ctgacgggga acaaggggta tggctttgcg
    20881 cgcagaaggg acgcttttct cttttcggtg tttacatcgt ccatgtgagc atcctggttt
    20941 tgatagcggg cgcgatcctt ggatcggttt tcggcgttga aggatatgtc cacattccgg
    21001 aaggggaaac ggcggaggcg atttccctgc gcagcggcgg tcagccgttg ccgcttccct
    21061 ttgccgtgcg ctgcgacaaa ttcaccgtgg agttttacga aagcggcatg cccaaaacgt
    21121 ttcagtccga cctgaccttt ctcaaggacg gcgcggcggt ccgaaccgcg aagcttctgg
    21181 tcaatcatcc cgttgaattt ggggggttcc gtttttatca ggcgagctac ggcgcggcgc
    21241 cgggaggcaa ggcgacgctg gcgctggcgc aggacggcgg ccgccgcgac gtgatgaatg
    21301 tcgcgcaggg gttcgtcttc gacctgccgg gcggggaggg aacgtttcag gtgctgcgcg
    21361 ttgaggaaaa tctgatgaag atgggccccg ccgtcaaggt agccgtcacc tcccgcaggg
    21421 gggaagaggc tgttttctgg gtgttccagc agatcgaacc cattaaaaaa atgagcccgg
    21481 acattttcga gcaggtgccg gtattcaatc ccgctctgtt tcgtccctac accttttcgc
    21541 ttttgggcct cgaggaaaaa tattacaccg gcttgcaggt caaccgcgac cccggaacgc
    21601 cggtggtggc ggtttccgcc ttgctgttga tcgggggcct gatgctgatt ctgttttcct
    21661 acgcccgcgc ggtttggatc cgtgtcgcgc catcggagaa tcatgtgctg gtcgcgattg
    21721 ccggccggag ctacaagaat cagccgggcc tgcagaaaga aatgcaatac ctgctcgcgg
    21781 agctgaagga ccatctggag aagcgccaat gatccatacg acgattttaa gctgggtgac
    21841 cttcctctat tttgccgctt ttgtttttta tcttttccgg atgattctgg ggcgcgagtt
    21901 ctggggaaag gcggccacgg gagcggcgct ggccggtttg gccgcccaga gcgttgcgct
    21961 tgccctccgc tggagggcgt cttacgatct gggtttcggt catgcgcccc tgtccaattt
    22021 ttacgaatct ctgatttttt tcgcctggac gatcgttctt ctgtacctgc tcatggagcg
    22081 gcgtctgaag aaccggagca tcggcgtttt cgttctgccc gttgcgtttc tgatcatggc
    22141 ctacgcgtcc atcgctccgg gaatgaatca gcgcatcgag ccgctgattc ccgccctgca
    22201 gagcaactgg ctgacctcgc acgtgttgac ctgttttatg ggatatgccg cgtttaccgt
    22261 ggccttcgcg ctgggggtca tgtattttgt caagaaagcg gccggtctca agcccggcgt
    22321 ctttatccgc ctgctgccgt ctgaatttca aatcgacgag ttgatgtatt cctgcgcggc
    22381 gctgggattc atctttctga cgctgggaat cgtgaccgga tccgtctggg cgcattatgc
    22441 atggggctct tactggagct gggatcccaa ggaaacctgg tcgctcatca cctggctgat
    22501 ctacgccgtg atgctgcata cccggctcgt gcgcgggtgg cggggccggc gcatggccgt
    22561 gatgtcgatg gtcggcttcg gctgcatgct cttcacgtat ctgggggtga atttattgcc
    22621 ctctttgcac agctacatga gctgacgcca ctcccaactc aaagcgctat ctttgttggc
    22681 tgagtcgtcg gcatcctcaa cgtatataaa aatacgcctc gtcgcctcct ctcagccgcc
    22741 gcgatatcat ctttgatttg ggagctgcgt tattcttaag aaatccatca tttcctggat
    22801 gtccggcagt tcccgaaggg gatagacttt gacgaagacg gcttttcgct tctcgtccag
    22861 aatgatgttg gcccgctcgg agaagccgag tttgtcgatg aagaggccca gcctcgcggc
    22921 gaggccgccg tggggccaga agtccgccag aagatcgact ttttcgatgc cgagattttt
    22981 agcccaggct tttttgcagg gagcgctgtc cacggagatt cccaacggaa cggtgttgtg
    23041 attccggaag tcgtccaggc gggcttccag cgactgcatc tgcttcgcgc agacttccgt
    23101 ccaggccagc ggatgaaagg agagcagaat tttttggccg gcaaactgcg ccgtatccac
    23161 cgcggcttcc gtgtgactct ttaacgagaa gtctccaaac acatctccga cttgaattcc
    23221 ggccatgatg cttctccttt cgtatttcgt cgttcgtatc tcgtgtctcg cgaaccctga
    23281 accctgaatt gttgaacctg tttcactcga tcgccatcag cgactgccag cgctcccatt
    23341 cggtggcgtg cggaaggccg cgcttctgca gcagccgaag gataggctcc tcgttgaatc
    23401 tcaggtaagg attcatcgtc ttttcctggg cgagagtgga gaagagaaaa tgcggatcgc
    23461 aggtttgccg gaaacgctcg atttcgggat tgtccggctc caaatgccgg gcgaaagcta
    23521 gcgaatccct cacataatca tggccggcga aaatgcgcgt gtcgtcgggc agggccatca
    23581 gacgctgaat ggaatcgaaa aatcctttaa gatcgccggt aaaacagttg ccgactgtcg
    23641 cgttgaagag agtgtcgccg gtcacgaggt tttgccccgc gtgaaagcat acggaatcca
    23701 tggaatggcc gggtgtccga tatgcgatga ccggttcgcc gtcgatgagg attttttctc
    23761 cgtcggcaaa cgccgtacag tcaagaaacc cggcttttgt ctttctcagc aggtgatcgt
    23821 cgccgggcgt gtggtcatag tgccggtgcg tgttggtgac gtacgcgagc ataagccggt
    23881 tctgctcgag aaacgcgagg atttcctgcc aggcgccgcc gtcgatcgcc agggcctccg
    23941 tcgggccgta cagcaggtag gccagattgt ctccgtagcg gaattgtacg atctggagca
    24001 tgccgttttc cctattttac gattttaaag atgtctctgg atttggaatc ggcggcgctg
    24061 ccgagcagtt caaacagaac catctccgcc gacgtgagat gagcgccgcc gctcctgatc
    24121 gcgtcgatgc cgatccggcg gttttccggc ttgcgcgacg aaacggcgtc ggcggccaga
    24181 tgaaccgtgt agccgttgcg aagaaggtcc agcgccgtct ggtaaacaca gacgtggctt
    24241 tcaatgccgg ccaggacgac atgccggcgg gtgagggatt cgagctcgtc gcggaatgag
    24301 ggattggccc agcagctgaa cgtctccttg gcaatgggtt ttccgcttcc cagctcccgg
    24361 gcgatcaggg gaatcgtcgt ccccagtttt tccggtgtct gctccgttgc cacaacgggc
    24421 aggttcagga tctgaaatcc ccggatcagc ttgacggtat tggcaaaaag ttcttccttg
    24481 cggtccatgg actgggccag gttgccctga aaatcgacca gaaccagcac ggcggcgctt
    24541 cggctgagca tgtttccccc ttttatggct gcttttgccg aacaatagtg aatttgcttt
    24601 ggggttgtca atcattatta ttgctggtaa taaggctaaa aagcctggag ataaaacatg
    24661 acgctgccgg ccaatgaaaa gattcttctg catgaatacc gggaaggccg ctttgcggaa
    24721 gtggagctgc ccttttccgc ggaatatgcg ctgacgctga atatcaacgg cagcccctac
    24781 gtcacgatgg cctgctccgg aaaggatctg cgggagcacg tgaccgggta tctgatcacg
    24841 gaaggaatca tttcgggcgc ggatcagatc gcaaggctgg aggttcatga aaaagatctg
    24901 gccgtcgacg tctttctcat gaatgaccgg atcgtcgcgg aaaggctcga acagatcaaa
    24961 accatttccg cggcgggggg aaggacccgc acgcggctgc ccggggagga actggtccgc
    25021 acgcggctgc ccggggtggg ggcggaggtc atcctggggg ccatgagcga gtttctgtcc
    25081 tattcgcggg agcatgaggc cacgcacggc gttcacagcg cggcgcttta ttcgctggag
    25141 ggcgggcggc tgatcttctt cgacgaaatc ggccgtcaca acgccatcga caaggtgatc
    25201 ggtcgcgcgg ccattcaggg catcagtctg gaagacaaga tgatctgctc gacggggcgc
    25261 atctcgagtg agatcgcctt caaaatcatc cacgcccgcg cgccggttct gatttcgcgc
    25321 gcctctccga ccacgctgag cgtgagcctt ctgcgccggt ttaatattct gaccgtcatc
    25381 cgcgccgtaa acggccggtt ctttgtcgta aacggcaaag aaagcattcg ggtatagcgt
    25441 ccgcttcggg cgcttcagga tgccgcccgg gagcgcgagg cgttttatct ttcgctcagt
    25501 tccagcaaaa tgccgccggt tgatttggga tgaacaaagg cgattttcgc gccgcctgcg
    25561 ccatagcggg gcttctcgtc gatcaggcga acgcctttgg ctttgagttc ggccaacgcc
    25621 gcttcaagat catccacccg cagggccatg tgctgaatgc cctcgccgtt tttttcaatg
    25681 tatttggcaa tgggtccgtc gggcgacgtg gattccagaa gctcgatttc cgaatctccc
    25741 acgggaaaaa aacaaacttt tactttctgc tcggccactt cttccgcgcc gactgaggcg
    25801 atgcccagca tctcgtaaag tttggcgctt tccgccagat tcttcaccgc gatgccgatg
    25861 tgatcgattt tcaaaacgtt catgctttcc atctccttgt attctgtctc gttgtttgtg
    25921 caggggacag ttttaaaccg ttccccgcgg atcgcccttc agtcatcagt ctaaaaatcc
    25981 ttttcaaaac ttctgccctt tcaggcactt caggacttgc gcggcggcgc tggtgggggt
    26041 gacaaggccc cgttcgactc cgtcgagccc ttcggccaga acctgcgccg ccgggctttc
    26101 gtagaaccat tcctgaacgc cttcgcgcac cagatatttc agccattccc tggcctgttc
    26161 cttgcgcttg gcctgcagct cgccggttgc ggtcagccgg tcgcggtggt cgaggatgat
    26221 gttcagaata ttctccagcc ccgccattgt gaccgaactg cacgtgacga cgggcggcgt
    26281 ccagtttgcg taaggcggcg taagcaggtg aaacgccgtt tcgtactggc ggcgcgccag
    26341 cttggcgcgc tcgacattgt ccccgtccgc cttgttgacc gccactgcgt cggccagctc
    26401 cagaaccccc ttcttgaggc cctgcagctc atcgccgcct ccggcgatca tcagcaccag
    26461 aaagaagtcg accatcgagg ccacggccac ttccgactgg cccacgccga cggtttcgac
    26521 catcactgcg tcaaatccag cagcctcgca aaccagcatg gtttcgcgcg tcttgcgggc
    26581 gacgccgccc agcgttttgc cggatgggga ggggcggatg aacgcccggt tgttcaccga
    26641 cagtttttcc atgcgggttt tatcggccag aatactgccg cccgttttgg gggagctggg
    26701 atcgaccgcc agaaccgcca cccggcagcc cctgtccgtc agcatggtgc cgaagctttc
    26761 aataaacgtg cttttgccgg cgcccggcag acccgtgatg cccaggcgca gggccttgcc
    26821 ggtatggggc atcagcgcgt ccagaatttg ccgcgaaagc tcctgatcgg agggcaggga
    26881 gctttcgacg agggtgatcg ttttggccag cattcgccgg tcccggtcga ggacgccctg
    26941 aatataagcc tgtccgtcgc gtttttccat catccttctt cctttgacgg atgctgatgg
    27001 ggttcagctt ctgtaaaatt cattgacggc cgccaggtat tccgccagat gacggcggct
    27061 gccgacaaag atgtgaccga catttttttc aatgcttcga t
//
LOCUS       DAOPAF010000019        26898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_1961_length_26898_cov_50.386134, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000019 DAOPAF010000000
VERSION     DAOPAF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 26898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26898
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_26898_cov_50.386134"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..102)
                     /locus_tag="PLQ77_03485"
     tRNA            complement(9..102)
                     /locus_tag="PLQ77_03485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Ser,seq:cga)
     gene            complement(166..259)
                     /locus_tag="PLQ77_03490"
     tRNA            complement(166..259)
                     /locus_tag="PLQ77_03490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Ser,seq:gga)
     gene            complement(265..738)
                     /gene="tadA"
                     /locus_tag="PLQ77_03495"
     CDS             complement(265..738)
                     /gene="tadA"
                     /locus_tag="PLQ77_03495"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPL09774.1"
                     /translation="MLTDEDCMNMALEEARKAFGQDEVPVGAVVANGDQILAVAHNAP
                     VSAGDATAHAEMIAIREACRASGNYRLPAARLYVTLEPCLMCAGAILQARLARVVFGA
                     RDPKAGAVFSLYRVLHDSRLNHKVEVTEGILAQACGEILSRFFKEKRIEAASRNA"
     gene            832..1392
                     /locus_tag="PLQ77_03500"
     CDS             832..1392
                     /locus_tag="PLQ77_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPL09775.1"
                     /translation="MDAKKIFLLPVIVSLMGHAALIAASSMVDLRDRDKASQLFMVQI
                     APPPAAADPERDHSPTGEKVAKDSQDAKPVPSGDREDTVDIGSADVKYAAYLSGVKQK
                     IMRIWKYPEAAYQQEEEGVVVVKMSIEASGRLSQLALMTSSGFIHLDSGTLEVVRAAA
                     PFQPLPRQYDLSRLHIIASFNYRVEP"
     gene            1450..2082
                     /gene="lepB"
                     /locus_tag="PLQ77_03505"
     CDS             1450..2082
                     /gene="lepB"
                     /locus_tag="PLQ77_03505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPL09776.1"
                     /translation="MKENKSKPAAAKTKIREFIEAILTALLIAAFIISFVVQAFKIPS
                     GSMIPTLLVGDHLFVNKFIYGVKLPYFRTTIIPVTDPKREDVIVFIYPEDRSKDFIKR
                     VIGVAGDKIEMKNKKLFINDRPYADPNGHYTDQAIFPAEAQPRDNFGPLVVPQDSFFV
                     MGDNRDHSLDSRFWGFVDLKDVQGKAFILYWSWNHEERNVRWNRIARLIQ"
     gene            2089..3165
                     /gene="mutY"
                     /locus_tag="PLQ77_03510"
     CDS             2089..3165
                     /gene="mutY"
                     /locus_tag="PLQ77_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPL09777.1"
                     /translation="MLKNPHETGGLPARRLLAWYKRCGRTLPWRETADPYRIWISEIM
                     LQQTRVDTVIPYYERFLKQFPDVFALARAPLQDVLKAWENLGYYSRARHLHEAAKIIT
                     GRFGGRIPNTLEAIRTLPGIGDYTAGAILSIAFERAVPAVDGNVRRILSRVFAVAKPI
                     NDPGEHRHFFELAASLVPKKRASGFNQALMDLGATVCRAKNPDCGACPLASLCLAHHR
                     HLQDRLPVMRKAPAIPRRQSVAAVLRNDKGRLLVVQRPAEGLLASFWKLPGGFLKDHE
                     NPEAGLRRLVREELGLSIRVGKKIASVDHAYTHFRVTLHVYEGFLFNGKPAAKRCPNR
                     QWAAAADLKKLPLSKIDRMAAKLI"
     gene            complement(3240..3674)
                     /locus_tag="PLQ77_03515"
     CDS             complement(3240..3674)
                     /locus_tag="PLQ77_03515"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPL09778.1"
                     /translation="MRRDMLEMIESNRLCVLATVSESGPHCSLMSYAASEDGTEFYMA
                     TDKNTKKYRNLAANPSVSLLIDSRADSAEGRGAATRALTVTGTVRRNLDEPGKKAALE
                     ALCRRHPDLKDFLAGSSVEVIAVKINSLQLLDGIADSHFEEV"
     gene            3817..5895
                     /locus_tag="PLQ77_03520"
     CDS             3817..5895
                     /locus_tag="PLQ77_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL09779.1"
                     /translation="MKKNLITLNEFEGASLLRQSGIPVIDGIPARTEEEAAAAAEKLG
                     FPVALKICSEAVVHKTERGGVALNIQDTAALDGAVREMKNKFADIPFAVLVQKMAPSG
                     AELILGCRRDPVFGPIVLAGIGGIFTEILRDTVIDLAPVDEKAALAMLRRLKGAPLLE
                     GYRSREPLDIAAAAKALSALSCLAAERADLLEIDVNPFIVYPRGAVAVDALVRLDAGP
                     AAVPRKRTSPATVAPFFQPSSMAVIGASRSPGKGGNIILRNLQKAGYRGALYPINPTV
                     KEILGLPSYARVSDVPKPVETAMVVIPKTAVPEALADCAAKGVTNIILSTGGYSDTGE
                     EGAKEQKIIIDMARKAGIRVMGPNSIGTINPSAGMSTSIVGLDPIRPGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDINESDLLEYLTGDSATRTVGMYLEGVVEGK
                     RFIRAAAAASKTKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRAGLLRVQN
                     SEAFFDALAAFEKLPLPRGNRMGALSITGMGCVAATDAAEECGVDLPALQKETLKKLG
                     EVMPVWAPVRNPIDTWSAIEQHGSKKATSHIARCLMEQPDVDALLITFVLMPESMFDI
                     SEAFTEIFRRHPHKPVFASYYGGTAREIAHIHEGFAALGVPCYPTPERAVFAFGRMVE
                     YARFRGVIRK"
     gene            5994..6773
                     /locus_tag="PLQ77_03525"
     CDS             5994..6773
                     /locus_tag="PLQ77_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09780.1"
                     /translation="MINHGRKLFFALVVALSLTVVAEANAFELGARAYLWFPDLKKAD
                     VQTIANGIEGSDISATDMLGVGNKITYSVEAYGGFGKNHVSLMYTPFGYTTDAILATA
                     LTYNGIPYKAGDTVHTDMGYSMFDLKYQRDLINLENVLAGFSLGGIAQIKYSTGSFKL
                     NSAGKLEQDRSFNSIIPMIGVGAHIGLLANLLEARAQVTAGGYSSENYSYEALADVSL
                     TPFPFLDIHAGYRLLQQKMDVNDYKMDTLYSGPYIGLSVGF"
     gene            6995..8434
                     /locus_tag="PLQ77_03530"
     CDS             6995..8434
                     /locus_tag="PLQ77_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL09781.1"
                     /translation="MERFFDPQSVVVVGASNTPFNLGSTICNMLKDYLHYDGNVYAVN
                     SKGEAVNGYPGYSSIPDLPQTPDLAVIIVAARHVPGIIRDCARKGIRHVVIESAGFSE
                     GGEIGEAMQREIDAIARQNGIRMLGPNCLGTLSSRNKFCCFYGVNPSLVEMNQIFESP
                     GSISYIIQSGGVVVLTLESLYYDLVGVNKVVSIGNKCDVDEADLIEYFQKDETEVIGI
                     YLENIADGRKLMEAARKSRKPVLVYKVGKTKEGAQAAMSHTAGMANNDRIFDAACRQA
                     GIIRLQSIDELHSLPKIFTEMPLLKGKRIAAFTNSGAFGGISADLLVQAGLQMVRLSP
                     QTQEKLKKVGQVFNIKNPVDIGPAPPQTYLDIFEILLSAEEVDGLLPLLSVWQPFVID
                     CLLELMKICKRYEKPAAIYTPNAISKSVAIRARHRLPIFETTEQAVRALSVSQTYFAS
                     LLKRNLTGDRPLWGAGLNTDHRSTGEALS"
     gene            8431..9135
                     /locus_tag="PLQ77_03535"
     CDS             8431..9135
                     /locus_tag="PLQ77_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL09782.1"
                     /translation="MTGKKEKEIKDMLLKAIAGSQLALSEFESKKAVAAAGVPVTREA
                     LVQSRDEAMSKAEKIGFPVVLKGSSPELTHKTEMGMVRVNLKNKDEAAAAYDELTGKG
                     IALEGVLIQEMVKGNREFVIGLIRDPHFGPCVMFGLGGIFTEAMKDVSFRVAPLTEED
                     AREMIGEIRAAKLLDAFRGEKAVDREILVRALVGIGNLGMKHGEIAEIDINPLIVKDG
                     KPVAVDALVILQAPRA"
     gene            complement(9332..10147)
                     /locus_tag="PLQ77_03540"
     CDS             complement(9332..10147)
                     /locus_tag="PLQ77_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPL09783.1"
                     /translation="MIVAGSYRRLGNCEMFAKDIALRARADSISLLRLTDFALKSCTG
                     CYRCLNPEHRCRVQDDLYFILEKMKGFDGIIFSIPTYVLGPVGQFKLFADRLACMAVF
                     QPDFRKIRAVSAIFGGIESWRGVTQSLVNAVIRMMGFDFRGSAFIEAALPGESLDVKY
                     QPVAQGLADALGENSAAYFPCKTLQCPSCGSDLFFIQDGRKVCVLCESSGEYRDGQWI
                     DYRDSKRFTARGFVEHFEGWLKPKVAEYSAQRERHRSLRETYKDIGVWIKKEE"
     gene            complement(10187..11026)
                     /locus_tag="PLQ77_03545"
     CDS             complement(10187..11026)
                     /locus_tag="PLQ77_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09784.1"
                     /translation="MSSKRAAFFFLSALLLCSAGCAAVFSQNDRYAAADRVADRAGFE
                     KSFIRTSSLTLTAFTRFTKKGDPLHVYIEGDGTAWLSRTRLSDDPTPRTPLVLELAAL
                     DSASNVAYLARPGQYAAEGVPDCDAAYWSEKRFAQEVVEAMNGALDELRRRAGAGAIH
                     LVGYSGGAAVAVLAAARRSDVAGLRTVAGNLDPEAVNRYHHVSAMKDSLNPMDAAEKV
                     RDLPQRHFVGSQDTVIPPGLARVFLKRVGCRDSSGITVVEGATHTRGWRERWRELLII
                     PLN"
     gene            complement(11016..13901)
                     /locus_tag="PLQ77_03550"
     CDS             complement(11016..13901)
                     /locus_tag="PLQ77_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HPL09785.1"
                     /translation="MKKHLCGLFWILLIALCLVEPAAAEMETFAQSVANAHANAAEPQ
                     GTLGRWIPTADSMYKVSTAGSVGANPYGLKYYGDPAQNQLIVRTASSENAYNGVYSGG
                     TITTAGSNYTIFGNKDVMSAWVTTGKEMTGFINSQGLTAGTFVKGVERGLGMNDRGNH
                     DAIFEMAVTVSNTANPYMLRPTRNPDPTQFSTDPATHGTNASFPATAQAAGIGTGAAA
                     DAVFANYKTAYTDWMTANYPWTQLGYTYYWGRAANKPENLSDVQGMSEFIMLGGAGGS
                     DTAKTPSGTDESGNVVVVGIYASQSYIYTKNDGTTFSDAPDAQYGNGFASFNVTGPCD
                     TLWAGRDFQVGASLNPAALPNTITIAADGSIAGGQGILVGSQNYTVTNAGSITANAGA
                     KKFNVAGSENIALLFKGDTHAAPGTLKNTLINSGTITAPGANGTAVAAWAGDTLIVNT
                     GTIIGTGTGYAIKTGAGNDTLTVNGGEIAGRVDLGTGTDNVNVPAGSSARFTFSLNTD
                     APAAAQIKSQTVAIADNTTTVAVRTTGTTNIKNNDQFLIADATTLTVDPAKLSVINDP
                     AFPMFTFSPQTSGSRLFLTALRDAAFYGQRVGNASLGYVLDSLANTATGDMAFVLGAL
                     DSSGDPAGAGQMQPTVNNGPIQASFGTVSQYTSTVLNRIEQVAAARTDGIGMTGISTG
                     DETAQGGVWAQGFGSYLHQGAQQASQGYTANVWGISFGYDRYLFQNIIAGFSGAFARN
                     NVTTGDVNTRTDADSYQGSLYGSLSKDAWYLDAILSFAYNRYDASRHIVFAGIDRTAK
                     SDYGGYQYSGYFEGGYTVKASGLLLTPLVSVQAMRLNLDDYTETQAGALNLKIDRQRY
                     NLFQTGLGAKVAYPVSKKNLKITPELHAKWLYDFAGDAQQAASTFTGGGASFVTTGFE
                     PPRTSGNIGAKLTILTRTNWSVSFNYDFEMKQDFYSHNGWVNLRYEF"
     gene            14122..15537
                     /locus_tag="PLQ77_03555"
     CDS             14122..15537
                     /locus_tag="PLQ77_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPL09786.1"
                     /translation="MSLSEELKYFDATELAGLIRKKEITPLELVESSIERIEKLNPAL
                     NAVITPMYDLARDAAASKLPDGPFTGVPFLLKDILASYGGVRMTFGAKVLKDLVPDHD
                     SELVARHKKAGLIVIGKTNVPEFGILPTTEPELFGPARNPWNTNCSTGGSSGGSAAAV
                     AARIVPMAHGNDGGGSIRIPASCCGVFGLKPTRGRITLGPDFGDMIGGLVNEHALTIS
                     VRDSAALLDATAGCLRGDPYWAPPVERPFVKEANTDPGRLRIAFAAKAPTDDPVHEDC
                     VRAVQDAARLCEELGHSVEEAAPVIDGKTLTHAFVVPWFAGIASIVKMLGVTKTQVEP
                     LTWAIYERSEASTAADYLIAVQTIQRVARDVASFFEQYDVLLTPTLTKPPAPLGSFEP
                     TPEDPLAGFAETARYAVFTPICNATGQPAMSVPLFWNSEGLPVGAHFIGRFGDEATLF
                     RLAGQLEKARPWKDRRPPVCA"
     gene            15550..16038
                     /locus_tag="PLQ77_03560"
     CDS             15550..16038
                     /locus_tag="PLQ77_03560"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HPL09787.1"
                     /translation="MQIFVDADAFPNGIRDILIKASLRLNIPLIFVANKPLHLEKLPN
                     LSLILVPEGPDVADDRIAQSVRPGDLVITADIPLADRVVDKNAFAMNPRGMLYTEHNI
                     KDRLAMRDLLGELRDNGMITGGPAAFSKKDRQAFANQLDSFLVRLMKPVKVMHQKPVS
                     DS"
     gene            16236..17867
                     /locus_tag="PLQ77_03565"
     CDS             16236..17867
                     /locus_tag="PLQ77_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPL09788.1"
                     /translation="MAQKKIIKKFIALMMILAFMLFSIGVWAAENPAAQKPDYDVIVI
                     GAGMGGLSAAAHLAVKGQKVLVLEKHFVVGGSTTKFTRGEFTFDAALHEMAGGGPGKM
                     DRGLYKLLKATGVDKKVELYEVPHFYRSIFPGVDITLPPNWPGFKSELKKKWPEESEG
                     IEKFHTLCSSLSAELMELRDLFRQGSVGGTLTQIMVPLRQRTFYKWKDRTVKDLMDAC
                     FKNEDLKAVVSQLWVFYGAPVDKESALIFMAATESYLSDGAWHIKGTSQALADGYAAR
                     IRELDGTVQTGTLVTKILLENGRATGVQTANGKTYTARYIVANTDPYQLADKLVGREN
                     LPAKYLEKIDAMKPANSLFGVYIGLNVDLKALGYNDTEIFYNTYRDSMKNYEAMMKGD
                     FEKGAVAITIYTNLGDPVYAPKGKSVVKLDAYSDISVWPKDRAEYAKLKEQKVDELIA
                     LAARVIPELKDPKNITVKEGYTPKTIERFTMNKGGVVYGFYLSPEQWQKIPNDTPIKN
                     VFITSNWTQAWHGVCAGQVNGWRAARLILDREGIE"
     gene            18002..18478
                     /locus_tag="PLQ77_03570"
     CDS             18002..18478
                     /locus_tag="PLQ77_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09789.1"
                     /translation="MLKDYQEKILELLEELELNMCNLYLLFADRFPKYRDLWADISRQ
                     ETSHADGIKKLHLLAGEDRVLFDEKLTKTYTVKMFITSIQEAQTRTKSGKMSLINALS
                     VSHDFEQAVLEKGFYNFFSGQDDETRTVIRQIKEETLDHQMKMKNAWEQERKSAGP"
     gene            complement(18489..19457)
                     /locus_tag="PLQ77_03575"
     CDS             complement(18489..19457)
                     /locus_tag="PLQ77_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09790.1"
                     /translation="MISEISENFYRITLPMPFRLRHVHAYMLVESGRAVLFDAGMLMP
                     GAFEKLEADLTGIGIAAADICDIYLTHTHTDHCGLAGRIQEKSKARLHLSNEARQVYD
                     RFQQGEDLIARARLFYARHGLPAEDVEAIVEEIEDLRGRIPLLEVATLLRDREIRRFG
                     SRAFEVIFTPGHAAGHVCFFFRDEGLLLAGDHILPYIAPSLSPNIDDEVFQPLASYLE
                     SLAVIEKLPISIIHPGHGNSFNGVHERIREIREHHALKKDMLQRRIGALPQTTFGICK
                     ETMRTGADGWEQFMALNETYVYLQHLKREGAIREGMVNNTLVYSAV"
     gene            complement(19486..20478)
                     /locus_tag="PLQ77_03580"
     CDS             complement(19486..20478)
                     /locus_tag="PLQ77_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPL09791.1"
                     /translation="MRIQKQYCWNGKGCRGAAGPSAARVWLRALRLHFVLPSILPALL
                     GGVMAWAAGHPPNGLHFALVVIGVTVNHFGLNLVDDVLDYRHTVDLQTGGEKNFFTGG
                     SGVLPEGLLKESRMLTAAALFFAATAGIGIYLAWACGWPVLALGIFGMACSIFYTAPP
                     IRFGYRGFGELGLLVNFGPVIVLGAYFVQARTLSFEPLIASLVPGLLMASMILINEIP
                     DYEADRRGGKWNLVARFGRKTGALLYGACLACACGILVLSSVAGVLSPFALLGLVSLP
                     FAAKSLAVLRRHLNDPLAMAPANLAMIKVHALTVAAMVAAYLLEVLIAARKFIC"
     gene            complement(20529..21197)
                     /locus_tag="PLQ77_03585"
     CDS             complement(20529..21197)
                     /locus_tag="PLQ77_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPL09792.1"
                     /translation="MNRNLALRILLLLALIALVGYLFIHFDLYLFFKDKNKIISFIQT
                     SRYDELVFIAIQIVQVVVAVIPGEISGFIGGYLYGPLLGTIYSTIGLTLGSWLAFMLA
                     RFFGEPLLEKVVKKEVFEKFDHFMEHKGLLVSFLLFLIPGFPKDYLCYIMGVSRIPAL
                     TFIILSTIGRFFGTMMLSVSGNMAREEHYAMLAVIAVAAAVVSFLAWRYHEKILELLK
                     NHKE"
     gene            21483..22844
                     /locus_tag="PLQ77_03590"
     CDS             21483..22844
                     /locus_tag="PLQ77_03590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL09793.1"
                     /translation="MSKTPQTLTRKKSEIYKNAPIARFGERKPDFTTMGRKIKNPHQK
                     FREVVCVEACRTPYGRAGGALKDFSAMELGALAIQEVLRRTEGKVKGADVDYIFMGQV
                     VPAGCGQIPGRQATILAGVPESVPCITVNKVCSSGIKTIDLAFQMILLGRAEICIAGG
                     QESMSNCPFTLPDMRWGMRMGLPNGRVVDTMVYDGLWDAFYNRHMAIHGSEVADEFGF
                     SREEQDEWALNSQQNAVKAMNEGKLDDEIFPVEVKQGKKVIIIDKDEGPRPETTLEGL
                     AKLPPVFGHLSTVTGKPGSVTAGNAPGVNDGGDVCLLMSREKADELGLQPIFTIVDYA
                     EVSQPTKDIATVPGLAIKKILDQNDMTLDQMDVIEINEAFAAVALVSCRSILGMTKEE
                     MFKKVNINGGAVAYGHPIGATGARIAMTLGYELKRRGGGWGVCGICSGHAQGDAMLIR
                     VDK"
     gene            23335..23988
                     /locus_tag="PLQ77_03595"
     CDS             23335..23988
                     /locus_tag="PLQ77_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09794.1"
                     /translation="MYLLPLGVDLRERFKRARTQAKSLSPSAQVVLLHTLLKSPQRIL
                     ALNDLALQYGYSAMTMSRAFSEIDSLGLFEISIRKRKRERILKGTKLEIWEKTLDYLR
                     SPVIRRLYADPSINLPDGVCAGLSALARYSALAEPGNKVLAVGNGTGKLLIQKYGREA
                     LSRKDVQDYEIEVWSYEPALFAMDGVVDRLSLFLSLRDEEDERVEAALEQMMREMQW"
     gene            23982..24740
                     /locus_tag="PLQ77_03600"
     CDS             23982..24740
                     /locus_tag="PLQ77_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09795.1"
                     /translation="MVKGLDVFQKHFQDYADRYILIGGTACDLALKEAGLSFRATKDL
                     DIVLCVEAIDRPFVKVFWAFIRAGGYQIQEKASGEKQFYRFTKPVDNSFPFMLELFSR
                     KPDTLSIDAEGHLTPIPIDDEAASLSAILLDDGYYFFITQGKRENNGLMVIGVDRLIP
                     LKAKAWLDLTQRKQKGEAIDSKNIRKHKNDVLRLYRIAETNTQVLLPDNITRDLQTFF
                     KAIIAEPTDLAALGLASTTLEAVIADLKKMYGMI"
     gene            complement(24758..26089)
                     /locus_tag="PLQ77_03605"
     CDS             complement(24758..26089)
                     /locus_tag="PLQ77_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09796.1"
                     /translation="MTSITPAPARWVMMVDSIEELLDGPHAGDVAELAEYGLQQAAEA
                     VQYMDDSDGGMGDVMYRLQELHLKSCEKEKQDPAKLAAHLFELELNSEWDIFSGAAKT
                     YASILGDKGLAVYRQLADEAWKEFAPSASKQELRRSNANAALNLIGIKETLAVISGDI
                     EELVDIKKRDLTYAYHYLEIAEIYKKAGNKDKALEWAEAGLKAFPARTDSRLREFLAD
                     EYHRRKRPAEALNLIWANFTDQLGLDSYQRLKKQADKTGQWSDWREKTLSHIRSHITK
                     QDRKTNRWYWNPGYSLLVEIFLWEKEPEAAWKEALEGGCSQSLWMKLAALRAQDHPLD
                     AVEVYMKYVNPTIEQTNNKAYEEAVQLIKKIQPLMFRQRKDEAFTAYVDELRTRYKAK
                     RNFIKLLDKIQQGTLASVPCIIIYLSTRMSMASPWAWPEQMPQTPRFCGNP"
     gene            26672..>26898
                     /locus_tag="PLQ77_03610"
     CDS             26672..>26898
                     /locus_tag="PLQ77_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPL09797.1"
                     /translation="MLFTLFTIFASSFVIALSGALMPGPLLTATISESSRRGFWAGPL
                     MIAGHAILELALVVALFLGLAPFFQMPAVFAA"
BASE COUNT         6059 a         7666 c         7474 g         5699 t
ORIGIN      
        1 aagatgaatg gcggagagtg ggggattcga acccccgtag gagcttgtgg cccctaaatc
       61 gatttcgagt cgatcccgtt acgaccactt cggtaactct ccggatggcg gactgttcaa
      121 aaaaacggta gagacgctgt ctgaagcgcc cctacctttt atttatggcg gagagagcgg
      181 gattcgaacc cgcggtacac ctttgtgggc gtacacacga tttccagtcg tgctccttcg
      241 gcctctcaga catctctccg cgcctcatgc attcctcgag gcggcttcta tcctcttttc
      301 cttaaaaaat ctactcaaaa tttcgccgca ggcctgggcg aggattccct ccgtcacctc
      361 caccttgtga ttcaggcggc tgtcgtgcaa aacacggtag agggaaaaga ccgcgccggc
      421 cttcggatcg cgcgcgccga acaccacccg cgccagccgc gcctgcagga tcgcgcccgc
      481 gcacatcagg cagggctcga gggtcacata aagccgcgcg gcaggcaggc ggtaattgcc
      541 cgacgcccgg caggcctcgc gaatcgcgat catctccgcg tgcgccgttg catcgcccgc
      601 ggaaaccggc gcgttatgcg ccacggccag aatttggtcg ccgtttgcca ccacggcgcc
      661 taccggaact tcgtcttgcc cgaaggcctt tcgggcctct tcgagcgcca tgttcatgca
      721 gtcttcatcc gtcagcatcg gcttcgcctt gttttaaagt cgttttttga ttatcatact
      781 gttgattttg aggcaaatga catatatgat cctgaacgcg aacgaaaggc catggacgcg
      841 aaaaaaatat tccttttgcc ggtgatcgta tccctgatgg gacatgccgc gctgattgcc
      901 gcgagcagca tggtggatct gcgcgaccgg gacaaagcct cgcagctctt catggttcag
      961 atcgcgccgc ctccggcggc ggccgatccg gaaagggatc attccccgac cggcgaaaaa
     1021 gttgcaaagg acagccagga cgcgaaaccg gtcccgtcgg gagacaggga ggacacggtg
     1081 gacatcggct ccgccgacgt caaatacgcg gcctacctgt cgggcgtcaa gcaaaagatc
     1141 atgcgcatct ggaaatatcc cgaagccgcg taccagcagg aggaggaagg agtggtggtg
     1201 gttaaaatgt cgattgaggc cagcggccgg ctttcgcaac tggccctgat gacttcctcc
     1261 ggcttcatcc acctcgattc cggcacgctg gaagttgtcc gggcggccgc gcctttccag
     1321 ccgctgcccc ggcagtacga cctgtcgcgt ctgcatatca tcgcctcttt caactaccgc
     1381 gtggaaccat agtagatacc tcgggaattc gcgcaaaacg ggaaaagcac acacctcttg
     1441 gggagaaaca tgaaagaaaa caaatcaaaa ccggcggccg ccaaaaccaa aatccgggag
     1501 tttatcgaag cgatcctgac cgcgcttctg atcgccgctt ttatcatttc cttcgtcgtt
     1561 caggcgttca agattccctc cggctcgatg attccgacgc tgctcgtcgg cgatcacctg
     1621 ttcgtgaata aattcattta cggcgtcaag ctcccgtact tccgcaccac catcatcccg
     1681 gtcacggatc cgaagcggga ggacgtcatt gtgttcattt atccggaaga ccgctccaag
     1741 gatttcatca agcgcgtcat cggcgtcgcg ggcgacaaaa ttgaaatgaa aaacaagaaa
     1801 cttttcatca atgaccggcc ctacgcggac cccaacggtc attacaccga ccaggcgatt
     1861 tttccggccg aggcacagcc gcgcgacaat ttcggcccgc tggtcgttcc ccaagattcg
     1921 tttttcgtga tgggcgacaa ccgcgatcac agcctggaca gccgcttctg gggattcgtc
     1981 gatctcaagg acgtccaggg aaaagccttc attctctact ggtcgtggaa ccacgaggag
     2041 cgaaacgtcc gatggaaccg catcgcccgg ctgatacaat agaaaaccat gttgaaaaat
     2101 ccgcatgaaa ccggtggcct tccggcccgc cgcctgctcg cctggtacaa acgctgcggg
     2161 cggactctgc cctggcggga gacggccgat ccctaccgca tctggatttc cgaaatcatg
     2221 ctgcagcaaa cccgtgtgga cacggtcatc ccctactacg agcgttttct gaaacaattc
     2281 cccgacgtct tcgcgctggc ccgcgccccc ctgcaggacg tgctgaaagc ctgggaaaat
     2341 ctgggctact attcccgcgc ccggcatctg cacgaggcgg caaagatcat caccggcagg
     2401 ttcggcgggc gaattcccaa cacgctcgaa gccatccgga cgctgcccgg catcggcgac
     2461 tacacggccg gggctatctt aagcatcgct ttcgaaaggg ccgttccggc cgtggacggc
     2521 aatgtccgcc ggattttgag ccgcgttttc gccgtcgcca aacccatcaa cgatcccggg
     2581 gaacacaggc attttttcga gctggccgca tccctggttc cgaagaaacg cgcctccggc
     2641 ttcaaccagg ccctgatgga cctgggggcg acggtctgcc gcgcgaaaaa tccggactgc
     2701 ggcgcctgcc ccctggcgtc gctctgcctg gcccatcacc gtcatctgca ggacaggctg
     2761 cccgtcatgc gcaaggcccc cgcaatcccg cgccgtcagt cagtcgccgc cgtcctcaga
     2821 aacgacaaag gacggcttct ggtggtgcag cggccggccg agggcctgct ggcatcgttt
     2881 tggaaactgc cgggcggttt tttgaaagat catgaaaacc cggaagccgg tctgcgccgt
     2941 cttgtccggg aagaactggg tctttccatc cgcgtcggga aaaaaatcgc ttccgtggat
     3001 catgcctaca cgcatttccg cgtgacgctt catgtttacg agggatttct gttcaatgga
     3061 aaaccggcgg caaaacgttg ccccaaccgg caatgggccg ccgcggcgga tttgaaaaaa
     3121 ctgcccctgt ccaaaatcga ccggatggcg gcaaagctga tctgagcacc ccgtcagcct
     3181 tccggcataa gcggaaaact ccggccggcg tttaccgctt cggctttcgg ttttctgact
     3241 caaacttctt caaaatggga atccgcaatc ccgtcgagca attgaagcga attgattttc
     3301 accgcaatca cttccacgga ggaaccggca agaaaatcct tcaggtcggg atggcgccgg
     3361 cacagggctt ccagagccgc tttttttccg ggctcatcca aattgcgccg aaccgttccc
     3421 gtgacggtca gcgccctggt agccgccccc cggccttcgg cgctgtctgc tctggaatcg
     3481 atcagaagac tgacggacgg attggccgcc aggttgcggt atttttttgt gttcttgtcc
     3541 gtggccatgt aaaattccgt gccgtcttca cttgcggcat acgacatcag cgagcagtga
     3601 ggcccgcttt ccgacacggt ggccagaacg cacaaccggt tggactcgat catttccagc
     3661 atatcccgtc gcatcttttt caccccttcg acccgcagaa tgatgcagat tcaatacagg
     3721 accggccgga atatgcaagt tgaattattt caaaatcatg atagggtcac cgcgtaaaat
     3781 tcggaaatac gcatgccgcg gggaggtatt tgcattttga agaagaatct gatcaccctc
     3841 aatgaatttg aaggagcgtc tctgctcagg caatcgggga ttcccgtcat tgacggcatt
     3901 ccggcccgca cggaggagga agcggcggcg gcggccgaaa agctcggatt tccggttgcc
     3961 ttgaaaattt gttccgaggc cgttgtgcac aaaaccgaaa ggggaggcgt cgctctcaac
     4021 attcaggata ctgccgccct ggacggggcc gtgagggaaa tgaaaaataa atttgccgat
     4081 attcccttcg cggttctggt gcagaagatg gccccttcgg gcgccgaact cattctgggg
     4141 tgccgccgcg atccagtttt cggcccgatt gttctggcgg gcatcggcgg catttttacg
     4201 gaaatcctgc gcgacacggt catcgacctt gcccccgtgg atgagaaggc ggcgctggcc
     4261 atgcttcggc gcctcaaagg cgctcccctg ctggaagggt accggtcccg ggagcctctc
     4321 gatatcgccg ccgccgcaaa agccctgtcc gcactttcct gtcttgccgc ggagcgcgcg
     4381 gatcttcttg aaatcgacgt caaccccttc atcgtttatc cgcggggcgc cgtggccgtc
     4441 gacgccctgg tgagactgga tgccggcccc gccgccgttc cccgcaagcg caccagcccg
     4501 gccaccgtcg cgcctttttt ccagccttcg tcgatggccg tgatcggggc ctcgcgcagc
     4561 ccgggaaaag gcggcaatat cattttgcga aacctgcaga aggccggcta caggggcgcg
     4621 ctttatccca tcaatccgac cgtcaaggaa atcctcggcc ttccctccta tgcgagagtg
     4681 agcgacgtgc caaagcccgt cgaaaccgcc atggtcgtga ttcccaagac ggccgtgccc
     4741 gaagcgctgg cggactgcgc cgcaaagggc gtgaccaata tcatcctgag cacgggcggc
     4801 tactcggaca cgggagagga aggggccaaa gaacaaaaaa tcatcatcga tatggcccgc
     4861 aaggccggca ttcgggtcat gggacccaac agcatcggca cgatcaaccc gtcggccggc
     4921 atgtccacct ccatcgtcgg cctcgatccg attcgtccgg gcggtgtttc catcatcggc
     4981 cagtccggcg ttttctcgtc cgggtgggcc cgctggatcg cggacaccaa gcccttcggt
     5041 ctggccaaag tggcctgcat cggcaacaag ggagacatca acgaaagcga cctgctggag
     5101 tacctgaccg gggattcggc cacccgcacc gtcggcatgt acctcgaagg cgtggtggaa
     5161 gggaaacggt tcattcgcgc cgcggccgcg gcatccaaaa caaaacccgt ggtggtgatg
     5221 aaagccggtc gcagcgaagc cggggccgcc gccgtggcgt cgcataccgg atcgctggcc
     5281 ggatcggacg cggtatttga cgcggtctgc cgcagagcag gtcttctgcg ggtgcaaaac
     5341 tccgaagctt tttttgacgc tctcgcggcc tttgaaaaac tccccctgcc gcgcggaaac
     5401 cgcatgggcg cgctctcgat caccggcatg ggctgcgtcg ccgccacgga cgccgccgag
     5461 gaatgcggcg tcgatctccc cgcgctgcag aaggagactc tcaaaaagct gggcgaggtc
     5521 atgccggttt gggcgccggt gcgcaatccc atcgacacct ggtccgccat cgagcagcac
     5581 gggtcaaaga aggccaccag ccacatcgcc cgctgcctga tggagcagcc ggatgtcgac
     5641 gcccttctga tcaccttcgt gctcatgccg gaaagcatgt tcgatatctc cgaagccttc
     5701 accgagattt tccggcgcca cccgcacaag ccggtctttg ccagctacta cggcggcacg
     5761 gcaagggaga tcgcccatat ccacgagggc ttcgccgcgc tgggcgttcc ctgctatcct
     5821 acgccggagc gcgccgtctt cgcctttggc cgcatggtgg aatacgcccg gttccggggc
     5881 gtcattcgga aataagcgcc gggattccgg gcaaatttcg ccgacacttc cgattgacga
     5941 atgccgggaa atatgcgaga ttgcaaacca taattcatca aatggaggat actatgatta
     6001 accatggaag aaagctgttt ttcgctttag tggttgcttt atcgttgacg gttgttgcgg
     6061 aggcgaacgc ttttgaactg ggcgcaagag cctacctgtg gtttcccgat cttaaaaaag
     6121 ccgatgtcca gaccatcgcc aacggcatag agggctcgga catcagcgcg acggacatgc
     6181 tgggggtcgg aaataaaatt acgtattccg tagaggcata cgggggcttc ggaaaaaatc
     6241 acgtaagcct gatgtacacg cctttcggtt atacgacgga tgccattctt gcaacggctc
     6301 tgacatacaa cggcattcct tacaaggccg gcgacaccgt tcatacggat atgggatatt
     6361 ccatgttcga tctcaaatac cagcgcgatc tgatcaatct ggaaaacgtc ctggcggggt
     6421 tttcactggg aggaatcgcc cagatcaagt attccacggg cagctttaag ctgaactcgg
     6481 ccggaaaatt ggaacaggac cgatccttca attccatcat tccgatgatc ggcgtcggcg
     6541 cccacatcgg gcttttggcc aacctgctgg aagcccgcgc ccaggtgacc gcaggcgggt
     6601 acagctctga aaactattcc tatgaagcct tggcggatgt gtcccttacc ccctttccgt
     6661 ttctcgacat ccacgcgggc tacaggctcc tccagcagaa aatggatgtc aacgattaca
     6721 aaatggacac cctctactca gggccctaca tcggcctgtc ggttgggttc taaagacatt
     6781 aagacgcatc agcccggcct gagcggcaaa gcattgccgt gcggccgggc ggatgcaaca
     6841 aaccattccg gacaaaacac cgtaagtccg cccaatccct tattcctgcc cgttttccag
     6901 ctgccttttt cctgctgcct ttttcctgct tgacaaaagc cgcctttttg cgttgtatgg
     6961 cggcgcgaaa attcgacttc aggcaggtat tcttatggaa cgcttcttcg atccgcaatc
     7021 ggttgttgtc gttggcgcct caaacacccc cttcaatctg ggctccacca tctgcaacat
     7081 gctcaaagat tatctccatt atgacggaaa cgtctatgcc gtgaactcca aaggagaggc
     7141 ggtaaacggc tatccgggct attcatcgat cccggatttg ccccaaaccc ccgatctggc
     7201 ggtcattatc gtggcggcca ggcacgttcc cggcatcatt cgggactgcg cccgcaaagg
     7261 catccggcac gtcgtgattg aaagcgccgg tttttccgaa ggcggggaaa tcggcgaggc
     7321 gatgcagcgc gaaatcgatg ccattgcccg gcaaaacggc atccgcatgc tggggcccaa
     7381 ctgcctgggg accttgagct cgcgcaacaa gttctgctgt ttttacggcg tcaacccgag
     7441 cctggtcgag atgaatcaaa ttttcgagtc tccgggcagc atctcctaca tcatccagag
     7501 cggcggcgtg gtcgtcctga cgctggagtc tctgtactac gacctggtcg gcgtgaacaa
     7561 agtggtcagc atcggcaaca agtgcgacgt ggacgaagcg gatctgatcg aatattttca
     7621 aaaggacgaa acggaagtca tcggcatcta tctggaaaat atcgcggacg gccgcaagct
     7681 catggaggcg gcccggaaat cccgcaagcc cgtcctggtt tacaaagtcg gcaaaaccaa
     7741 agaaggcgcg caggccgcca tgtcgcacac ggctggcatg gccaataacg accggatctt
     7801 cgatgccgcc tgccgacagg cggggatcat ccgcctccag tccatcgacg aactccattc
     7861 gctccccaag atattcaccg aaatgccgct gctgaaagga aagcggatcg cggcgtttac
     7921 caattccggc gccttcggcg ggatttccgc ggacctgctg gtgcaggcgg gtctgcagat
     7981 ggttcgcctg tccccgcaaa cccaggagaa gctgaaaaaa gtcggtcagg tcttcaacat
     8041 aaaaaatccg gtggacatcg gccccgcgcc gcctcagacg tacctcgaca ttttcgagat
     8101 actgctctcc gcggaggaag tggacggcct tctgcccctg ctcagcgtct ggcagccttt
     8161 tgtgatcgac tgccttctgg agctgatgaa gatctgcaag cgctacgaaa agcccgccgc
     8221 catctacacg ccgaatgcca tctccaaatc cgttgccatt cgggccaggc accgccttcc
     8281 catctttgag acaacggaac aggccgtgcg ggccctgtcc gtttcccaaa cgtacttcgc
     8341 ctctcttttg aaaaggaatt tgaccggcga cagacctttg tggggcgccg gcttgaacac
     8401 ggatcatcgc tcaaccgggg aggccttgtc atgaccggca aaaaagaaaa agaaataaaa
     8461 gacatgctgc tgaaagctat tgcgggcagc cagctggccc tttcagagtt tgaatccaag
     8521 aaagctgtcg ccgccgccgg cgtgcccgta acccgtgaag cgctggttca atcgcgcgac
     8581 gaagccatgt caaaagcgga gaaaatcggt ttccccgtag tcctcaaggg atcatcaccg
     8641 gagctgacgc acaagaccga gatgggaatg gtccgcgtga atcttaaaaa caaggacgag
     8701 gcggccgcgg cctatgatga actgacggga aagggaattg ccctggaagg cgttttgatc
     8761 caggaaatgg tgaagggcaa ccgggaattc gtcatcggtc tgatccgcga cccgcatttc
     8821 ggcccctgcg tcatgttcgg cctgggcggc atttttacgg aagccatgaa ggacgtcagt
     8881 ttccgtgtcg cgcctctcac cgaagaggac gcccgcgaaa tgatcggcga aatccgcgcg
     8941 gcaaagcttc tggatgcctt ccggggtgaa aaagccgttg accgcgaaat actggttcgc
     9001 gctttggtgg ggataggcaa tctgggaatg aagcacgggg agattgccga aatcgacatc
     9061 aaccccctga ttgtgaagga cggcaagccg gtggccgtgg acgccctggt gattctgcag
     9121 gccccccgcg cctgatgaac atccatgaac cttgaacttt gatgcacctg taaaaagtca
     9181 taatttaccc acattgtcat ggtgaactcg tcgaaccatg cagtagcatc gaatgcttaa
     9241 gatcaccctt cgacgggctc agggtgacaa atgtgacttt ttacgagttt gtcaactttg
     9301 agcgggccgc tgtttttttg cggcccgttt tctactcttc ttttttaatc cagacgccga
     9361 tatctttgta agtttcccga agcgagcggt ggcgctctct ttgggcgctg tattccgcaa
     9421 ccttgggttt gagccagcct tcaaagtgct cgacaaaacc cctggcggta aaccgtttgg
     9481 aatcccgata gtcaatccat tggccgtccc ggtattcgcc tgagctttcg cacaggacgc
     9541 aaacctttcg cccgtcctga ataaaaaaaa gatcgctgcc gcaggaaggg cattgcaggg
     9601 ttttgcaggg aaagtaggcg gcggaatttt cccccagggc gtcggccagc ccctgcgcca
     9661 cgggctggta tttcacatca agactttcgc caggcaacgc ggcttcaata aaggcgcttc
     9721 cccgaaagtc gaagcccatc atgcgaatca cggcattgac cagagactgc gttactcccc
     9781 gccagctttc aatgccgccg aaaatcgcgc tgaccgcccg aattttccgg aagtcgggct
     9841 ggaacacggc catgcaggcc aggcggtcgg caaacagctt gaactgcccg accggcccca
     9901 atacataggt cggtatggaa aagatgattc cgtcgaaccc tttcattttt tcaagaataa
     9961 agtacagatc gtcctggacg cggcagcggt gctccggatt gaggcagcgg taacagcccg
    10021 tgcaggattt caaggcaaag tccgtaagac gaagcaaact aatggaatcg gcgcgggcgc
    10081 gaagggcgat atcttttgca aacatttcac agtttcccag acgcctgtat gaacctgcaa
    10141 caatcaaaag tgaagtcccc aaatctgccc tcctgatatc taatgctcaa ttcaaaggaa
    10201 tgatcagcaa ttctctccag cgctcccgcc atccgcgggt gtgggttgcg ccttcgacaa
    10261 cggttatccc gctcgaatcc cggcagccga cacgttttaa aaaaacccgg gcaagcccgg
    10321 gcggaatgac cgtgtcctgc gaacccacga aatggcgctg gggaagatcc cggacctttt
    10381 cggctgcgtc catcgggttt aaagaatcct tcatggcact gacatgatgg taacgattca
    10441 ccgcttccgg atcaagattt ccggccacgg tcctcaggcc ggccacgtca cttcgccttg
    10501 cggcagccag aaccgcgacg gccgctccgc cggaataacc gaccagatgg atcgctccgg
    10561 cgccggcccg tcggcgcagt tcgtccagag ccccgttcat tgcttccacc acctcttgcg
    10621 cgaaacgctt ctccgaccag taagccgcat cgcaatccgg gacgccttcc gcggcatatt
    10681 gcccgggacg cgccagataa gcgacgtttg acgctgaatc cagcgccgcc agttccagaa
    10741 ccagaggggt tcggggagtg ggatcgtccg aaagccgggt gcgcgaaagc cacgccgtcc
    10801 cgtctccttc gatgtaaaca tgcaggggat cacccttctt tgtgaatctc gtaaaggcag
    10861 tcagtgttaa cgatgaagtt cgaatgaagg atttctcaaa tcccgcgcgg tcagccacac
    10921 gatcggccgc cgcataccgg tcgttttgcg aaaaaacagc cgcgcagccc gccgaacaca
    10981 aaagcaaggc ggacagaaag aagaaggcgg ctcttttaga actcataacg caagttgacc
    11041 cagccgttgt ggctgtaaaa atcctgcttc atttcaaaat cgtagttgaa tgaaacgctc
    11101 cagttggtcc gggtcagaat ggtcagcttc gcgccgatgt tgccgctggt ccgcggcggc
    11161 tcaaaaccgg tcgtgacaaa ggaagcgcct ccgccggtga acgttgaggc ggcctgctgc
    11221 gcgtcgcccg caaagtcata gagccacttg gcatgcagct ccggcgtgat tttcagattc
    11281 tttttcgaaa ccgggtaggc gactttcgcg cccaggcccg tctggaagag gttgtagcgc
    11341 tggcggtcta ttttcagatt cagagcgccg gcttgtgttt ccgtatagtc atccagattc
    11401 aggcgcatcg cctgcacgga gaccagcggc gtcaggagaa gcccggaggc tttgaccgtg
    11461 tatccgcctt cgaaatagcc ggagtattga tacccgccgt aatcgctctt tgccgtccgg
    11521 tcgattccgg caaaaacgat atgccgggag gcgtcatacc ggttgtaggc aaacgacagg
    11581 atggcgtcaa gataccaggc gtctttggac agactgccgt aaaggctgcc ctggtagctg
    11641 tcggcgtcgg tgcgggtgtt cacgtcgccc gtcgtaacgt tgtttcgggc aaacgcgccg
    11701 ctgaagcccg cgatgatgtt ttgaaaaaga tatctgtcat acccgaagga aattccccag
    11761 acattcgccg tgtagccttg gcttgcctgc tgagctccct ggtgcaggta gctgccgaat
    11821 ccctgcgccc agacgccgcc ttgggcggtt tcatccccgg tggagatgcc ggtcatgccg
    11881 atcccgtccg tccgggccgc tgcaacttgt tcgatgcggt tcagaacggt gctggtgtat
    11941 tggctgactg tcccgaaact ggcctgaatc ggaccgttgt tgacggtggg ctgcatttgc
    12001 ccagcgcctg ccggatcgcc ggatgaatcc agtgcgccaa ggacaaaagc catatccccc
    12061 gtggcggtgt tcgccaggct gtccaggaca taacccagcg aggcgttgcc gactctttgt
    12121 ccatagaagg cggcgtcgcg aagcgccgta aggaaaagcc ggctgccgct tgtttgggga
    12181 gaaaaagtga acatgggaaa cgcagggtca ttaatgacgg atagttttgc cggatcaacc
    12241 gtcaacgtgg tcgcatccgc aatcaaaaac tgatcgttgt ttttaatgtt ggttgtcccg
    12301 gtggttctta cggctaccgt tgtcgtgttg tcggcaatag caacggtttg agatttgatc
    12361 tgtgcggcag ccggcgcatc ggtatttaag gagaaagtaa atctggcgct ggaacctgcg
    12421 ggtacgttca cattgtccgt ccccgtcccc agatcgaccc tgccggctat ctctccgccg
    12481 ttgaccgtca atgtgtcatt tcccgcgccg gtttttatgg cgtaaccggt tccggttccg
    12541 atgatcgttc cggtgttgac gatgagagta tcgccggccc aggcggcgac ggccgtgccg
    12601 ttggcgccgg gagccgtaat cgtgccggaa ttgatcaacg tgtttttcaa ggtgcctggt
    12661 gccgcatggg tatcgccctt gaacagaagc gcgatgtttt cacttccggc gacgttaaac
    12721 ttctttgcgc ccgcattggc ggtgatcgat ccggcgtttg tgaccgtata gttttgggac
    12781 ccgaccagga tgccctgccc gccggcaatc gatccgtcgg cggcgatggt aatcgtgttg
    12841 ggcaaggcgg cgggattcaa acttgcgccg acttgaaaat cccttccggc ccagagcgtg
    12901 tcgcaggggc cggtgacatt gaagctggca aatccgtttc cgtattgagc gtccggcgca
    12961 tcgctgaagg tcgttccgtc gtttttcgta tagatatagg actgtgacgc gtagatgccg
    13021 acgacgacca catttcccga ctcatccgtg ccggaagggg ttttggccgt atcggagccg
    13081 cccgcgccgc ccagcatgat gaactcggac attccctgca cgtccgagag gttctcgggt
    13141 ttattggcag cccggcccca ataataggta tagcccaact gcgtccaggg atagtttgcc
    13201 gtcatccagt cggtgtaggc cgtcttgtag ttggcgaaca ccgcatccgc agccgcgccg
    13261 gttccgatgc ccgccgcctg ggcggtagcg gggaaagaag cattcgttcc gtgtgtggcg
    13321 ggatccgtgc tgaattgcgt cgggtcggga ttccgtgtcg gccgcagcat atagggatta
    13381 gccgtgttgc tgacggttac ggccatttcg aagatggcgt catgattccc cctgtcgttc
    13441 atgccaagtc cccgttcgac tcctttgaca aacgtgccgg cggtcagacc ctggctgttg
    13501 atgaatcccg tcatctcctt tcccgttgta acccaggcgc tcatgacgtc cttgttgccg
    13561 aaaatggtgt agttggatcc ggcagtcgtt attgttcccc cggaatacac accgttatag
    13621 gcattttctg aactggccgt gcggacaatc aactgatttt gagccggatc accgtaatat
    13681 ttcaggccgt agggattggc cccgaccgag ccggcggtgg aaaccttgta catgctgtcc
    13741 gccgtcggga tccatctccc cagcgtcccc tgcggctcgg cggcgttcgc gtgggcgttt
    13801 gccaccgatt gggcgaaggt ttccatctcc gccgcagcgg gctcgaccag gcaaagagca
    13861 atcagaagaa tccagaataa accgcaaaga tgttttttca tatttacctc tcagcctcga
    13921 aaaaagatgc aaccggccgc tctgaaaaag aatgaaatcc aagcgtggtt gtttgcgcgg
    13981 gagtataaaa aacaccccgg ccgctgtcaa gcgaatacaa gggccaaata ctacaagagc
    14041 caaatactgc ttgacggtta aaaacggtcc ctttatactt cgtggcgtca aaggcaaaat
    14101 atcaccgggt aaggaggtcg tatgtcgctg tcggaggaac tgaaatattt tgatgccaca
    14161 gaactggccg gtctgatccg gaaaaaagag atcacgccgc ttgagcttgt cgaatcttcg
    14221 attgaacgga tcgagaagct gaatccggcg ctcaacgcgg tgatcacgcc gatgtacgat
    14281 ctggcgcggg acgccgcagc atcaaagctg cccgacggcc cctttaccgg cgtgccgttt
    14341 cttctcaagg atattctggc gtcctacggc ggcgtgcgca tgacgttcgg cgccaaagtt
    14401 ttgaaagacc ttgtcccgga tcatgacagc gaactggtcg cccgccacaa aaaggctggc
    14461 ctgattgtga tcggcaaaac caacgtcccg gaattcggca tccttccgac cactgagccg
    14521 gaactcttcg gccctgcccg aaatccatgg aacaccaact gctcaaccgg cggctcaagc
    14581 ggcggctcgg ccgcggcggt tgcggcgcgc attgtcccca tggcgcacgg caacgacggc
    14641 ggcggatcga ttcgaattcc cgcctcctgc tgcggcgttt tcggcctgaa gccgacccgg
    14701 ggcagaatca ctctggggcc cgatttcggc gacatgatcg gcggactggt caatgaacat
    14761 gccttgacga tttccgtccg ggacagcgcg gctctgctgg acgcgacggc gggctgcctg
    14821 aggggcgatc cctactgggc gccgcccgtg gaacggccgt ttgtgaagga agcgaatacg
    14881 gatccgggac gcctgcgcat cgcctttgcc gcaaaagccc ccaccgacga cccggtgcat
    14941 gaagattgcg tccgcgcggt tcaggatgcg gcccggctgt gcgaggagct tggccattcg
    15001 gttgaggagg ccgcccccgt catcgacggg aaaaccctga cccacgcctt tgtcgtcccg
    15061 tggttcgccg gcatcgcatc catcgtcaag atgcttgggg tgacgaaaac acaggtcgaa
    15121 cccctgacct gggcaattta cgaacgcagc gaagcttcaa ccgcagccga ctacctgatc
    15181 gccgtacaga ccatccagcg ggtcgcccgc gatgtcgcct cgttctttga acagtatgac
    15241 gttttgctga ccccgacgct gacaaaaccg ccggcgccgc tgggctcgtt tgaacccact
    15301 ccggaagatc cgctcgcggg atttgccgag acggcccgct acgccgtttt cacgccgatc
    15361 tgcaacgcga ccggccagcc ggccatgtcg gtgccgcttt tctggaacag cgaaggactg
    15421 ccggtgggcg cccatttcat cggccggttc ggcgacgaag ccacgctgtt tcggctagcc
    15481 ggccagctcg aaaaggccag accatggaaa gacaggcgtc cacccgtttg cgcataggat
    15541 taagattcca tgcagatatt cgtcgacgcg gatgctttcc cgaacggaat cagagacatt
    15601 ctgattaaag catctttacg cttgaatatt ccgctgatct ttgtggccaa taaaccgttg
    15661 cacctggaga aactgcccaa cctttctttg atcctggttc ccgaaggacc ggatgtggcc
    15721 gatgaccgca tcgcccaatc cgtgcgcccg ggcgatcttg tcatcacggc cgacatcccg
    15781 cttgccgatc gcgtggtgga caaaaatgct tttgcgatga atccccgcgg catgctctac
    15841 acggagcata acatcaaaga ccggctggcc atgcgggatt tgctgggtga gttgcgggac
    15901 aacggcatga ttaccggcgg cccggcggcc ttcagcaaga aagaccgcca ggccttcgcc
    15961 aatcagcttg atagcttttt agtccggcta atgaaacccg taaaagtgat gcaccaaaaa
    16021 ccggtatccg actcataaca caaacacgaa tcatcacgtc cggtagtacg agtctttaga
    16081 tccgctgttt tggatggtgg atggtggatg gtggatagtg aatagaaggg caaagcgcac
    16141 ccagatgtcg aaatggcaat ttgaacacca ggcaaacttg acaagcggtc gctttaatgg
    16201 gatgattcgc acaaattttt cccgggaggt ttctgatggc tcagaaaaaa ataatcaaaa
    16261 aattcatagc gttgatgatg attctggcgt tcatgttgtt cagcatcggc gtatgggcgg
    16321 cggaaaatcc ggcggcgcag aaacccgact atgacgttat cgtcatcggg gccggcatgg
    16381 gagggctttc cgcggcggcg cacctggccg tcaaggggca aaaagtgctg gttctggaaa
    16441 agcactttgt ggtgggcggc agcacaacca aatttacacg cggagaattt acctttgacg
    16501 cggcgctgca tgaaatggcc ggcggcggcc cgggcaagat ggacaggggg ctgtataaac
    16561 tgctcaaagc taccggtgtt gacaaaaaag tggagctcta tgaagtcccg catttttacc
    16621 gatccatttt ccccggcgtg gacatcacgc ttcctccgaa ctggccgggg ttcaaaagcg
    16681 aattgaaaaa gaaatggccc gaggagtctg agggcataga aaaatttcac acgctctgct
    16741 ccagcctgtc tgcggaattg atggaacttc gggacctctt ccgtcagggc agcgtcggcg
    16801 gcacgctcac gcaaatcatg gttccgctcc gtcagagaac cttttataaa tggaaagacc
    16861 gcacggtgaa agacctgatg gatgcctgct ttaaaaacga ggatcttaaa gcggtggttt
    16921 cccagctatg ggttttctac ggcgctccgg tggacaagga atcggcgctc attttcatgg
    16981 cggccaccga aagctacctg agcgacggcg cctggcatat caaaggcaca tcccaggcgc
    17041 tggccgacgg ttacgcggcc cgcattcgcg agctggacgg aaccgtccaa acgggcacgc
    17101 tggtgacgaa aatccttctg gaaaacggcc gggcaaccgg cgtccaaacc gcgaacggca
    17161 aaacctacac ggcccgctat attgtcgcca acacggatcc ctatcaactg gccgacaagc
    17221 tcgtcggccg ggaaaatctt ccggctaaat acctggaaaa aatcgacgcg atgaagcccg
    17281 ccaattccct gttcggcgtt tatatcggcc tgaatgtcga tctgaaagcc ctgggctaca
    17341 acgacacgga aatcttctac aacacctacc gggacagcat gaagaattat gaagccatga
    17401 tgaagggcga cttcgaaaag ggagcggtgg ccatcacgat ctatacaaac ctgggcgatc
    17461 ccgtctatgc gccgaaaggc aaatccgtgg tcaagctgga cgcctattcc gacatctccg
    17521 tctggccgaa ggaccgggcg gaatatgcca aactgaagga gcaaaaggtt gacgagctca
    17581 tcgctctggc cgcccgggtg atccccgaac tcaaagaccc caaaaacatc accgtcaagg
    17641 aaggctacac gccgaaaacc atcgagcgct tcacaatgaa caaaggcggc gtggtttacg
    17701 gcttttatct gtcgccggaa cagtggcaga aaattcccaa cgacacgccg attaaaaatg
    17761 tattcatcac cagcaactgg acgcaggcgt ggcacggcgt atgcgccggc caggtcaacg
    17821 gctggcgcgc ggcgcgtctg attctggacc gggaaggcat cgagtaaacc ggacgacggc
    17881 tctgcgggcg ccgcggaggt gaagatttcc gcggccccgc tctgatgatt ttttcaggca
    17941 gcttttatgc ttcgacgccc gggggcgggc gaatattgca agtcggcgga ggtaatcggc
    18001 gatgctgaag gattatcagg aaaaaatatt ggagcttctg gaagagctcg aattgaacat
    18061 gtgcaatctt tatctgctgt ttgccgaccg cttccccaaa tacagagatt tatgggccga
    18121 catctcccgc caggaaacca gccacgcgga tggaataaaa aaattgcacc ttctggccgg
    18181 tgaagatcgc gttctgtttg atgaaaagct gaccaaaacc tacacggtca agatgtttat
    18241 caccagcatt caggaagcgc agacccggac aaaatcgggc aaaatgtcgc tgatcaacgc
    18301 tctgtcggtc agtcatgatt tcgaacaggc cgttcttgaa aaaggatttt ataatttttt
    18361 ttccggccag gacgatgaaa ccagaacggt gatccggcag atcaaagaag aaacgctcga
    18421 tcaccagatg aaaatgaaaa acgcctggga gcaggaaaga aaatcggcgg gcccataatt
    18481 ccgaattgtc aaaccgccga ataaacaagg gtattattca ccataccttc acgaattgcg
    18541 ccttcccgtt tgaggtgctg caggtaaacg taagtttcgt tgagcgccat gaattgctcc
    18601 cagccgtccg cgcccgttct catcgtttct ttgcagatgc cgaaagtggt ttgggggagg
    18661 gcgccaatcc ttctttgcag catgtctttt ttcagcgcgt ggtgttcccg aatctcccga
    18721 atccgctcgt gaacgccgtt gaaagaattt ccatgccccg gatgaatgat gctgatgggc
    18781 agtttttcaa tgactgccag cgattccagg tagctcgcca gcggctggaa aacctcatcg
    18841 tcgatgttcg ggcttaagct cggcgcaatg tagggcagaa tatgatcccc cgccagcaaa
    18901 agcccctcgt cacgaaaaaa gaagcagaca tgccccgctg cgtgccccgg cgtgaagatc
    18961 acctcaaacg cccggctgcc aaagcgccgg atttcccggt cccgcaaaag cgtggcaacc
    19021 tcgaggagcg gaatccgtcc gcgcaggtct tcaatttcct ccacgatggc ttccacatct
    19081 tccgcgggca agccgtgccg ggcgtaaaag aggcgggccc gcgcgatcag gtcttctccc
    19141 tgctgaaaac ggtcataaac ctgccgggct tcattcgaca gatgaaggcg ggcttttgat
    19201 ttttcctgga ttcggccggc caggccgcag tgatcggtat gcgtgtgggt caggtaaata
    19261 tcgcaaatat cggccgcggc aatgccgatg cccgtcagat ccgcttccaa tttctcaaag
    19321 gcgccgggca tgagcatacc cgcatcgaag agaaccgccc ggccgctttc caccagcatg
    19381 taggcgtgga cgtgccgcag gcgaaacggc atgggcaggg tgatgcgata gaagttttcg
    19441 gaaatttcgg aaatcatcaa aggcagcctt atggtttgtg aatgtttaac aaataaattt
    19501 ccgggctgca atcaaaactt ccagaagata ggcggcaacc atggcggcaa ccgtgagggc
    19561 gtggacttta atcatcgcca gattggccgg cgccatcgcc agcggatcgt tcaggtggcg
    19621 tcgcaggacg gcaagggact ttgcggcaaa gggaagactg acaaggccca agagcgcaaa
    19681 aggggacaaa accccggcca cggacgagag gaccagaatg ccgcaggcgc acgccaggca
    19741 ggccccataa aggagcgcac ccgtttttct tccgaagcgg gcaacgaggt tccatttccc
    19801 gccgcggcgg tccgcctcgt aatcgggaat ctcgttgatg aggatcatcg acgccatcag
    19861 cagccccggc acgagcgatg cgatcagcgg ttcgaaagac agcgtccgcg cttgaacaaa
    19921 atacgccccc agaacgatca cggggccgaa attgacgagc aggcccagtt cgccgaatcc
    19981 ccggtagccg aagcggatgg gcggcgcggt gtaaaaaatg gagcaggcca tgccaaaaat
    20041 cccgagcgcc agcaccggcc acccgcaagc ccacgccaga taaatgccga tccctgccgt
    20101 ggccgcaaaa aaaagcgcgg ccgccgtcag catccggctt tctttcagca atccctccgg
    20161 caggacgccg ctgcccccgg tgaaaaaatt cttttcgccg ccggtctgca aatcaaccgt
    20221 gtgccggtaa tccagaacat catccaccag attgagtccg aaatggttga cggtaacgcc
    20281 gatcacgacc agggcaaaat gaagaccgtt tggaggatgg cccgcggccc acgccatgac
    20341 gccgcccagc agcgccggca gaatgctggg caggacaaaa tggaggcgca gcgcccgaag
    20401 ccagacccgg gcggcggacg gaccggccgc gccccggcat ccctttccat tccagcaata
    20461 ttgtttttga attctcatga agggccttca tcacatccgc acgggccgta ccgcaagagc
    20521 gtcggctctt attccttatg gtttttgagc agttccagaa ttttctcgtg ataccgccag
    20581 gccagaaacg aaaccacggc ggccgcgacg gcaatgaccg ccagcatcgc ataatgctct
    20641 tcgcgcgcca tgttgccgct gaccgacagc atcatcgtgc cgaaaaaccg gccgatcgtg
    20701 gaaaggatga taaaggtcag cgccgggatg cggctgaccc ccatgatgta gcacagataa
    20761 tccttgggaa agccgggaat caaaaacagc aggaaggaaa ccagcagacc cttgtgctcc
    20821 ataaaatgat caaatttctc aaaaacctct ttcttcacca ctttttccag aagcggttca
    20881 ccgaaaaacc gggccagcat gaaagccagc caggaaccca gcgtcagccc gatcgtggaa
    20941 tagatcgtcc ccagcagggg accgtacaga tagccgccga tgaaaccgct gatttctccg
    21001 ggaatgacgg ccaccaccac ctgcacgatc tggatcgcga taaaaacgag ctcgtcgtag
    21061 cgggaggttt gaatgaatga aataattttg tttttgtctt tgaagaaaag ataaaggtca
    21121 aagtgaataa aaagatagcc gaccagggca atcagcgcga gaagcaaaag aatccgcaag
    21181 gccaaatttc tgttcatgga gggtctcgaa tttccgatct gtcgcgcaat ggtttttaca
    21241 gcttgtaaaa cccgcggtgt gcctggcggt gttcgaaaat tttataatat gaatatcggc
    21301 tttcgtcaaa cggtttcggc tgtcttccgc gcgccgtcag gaaaggcaaa gggcttcacg
    21361 caacatcctg gtgcgcttca aaacagttga ctaaatgaaa gtgatgtgca agaaaaacgc
    21421 atctttgaaa acattcgcgt atttatcgcg catccggaca aaccgtcaac caggagggca
    21481 tcatgtcaaa aacgccgcaa acgctgaccc gaaaaaaatc tgaaatttat aaaaacgcgc
    21541 ccatcgcccg gttcggcgaa agaaagccgg acttcacgac catgggcaga aaaataaaga
    21601 atccgcatca gaagttccga gaagtcgttt gcgtggaagc ctgccggacg ccttacggaa
    21661 gggccggcgg cgccctgaaa gatttttcgg cgatggagct gggggcgctg gccattcagg
    21721 aagttctgcg ccgcacggaa ggcaaggtca aaggggcgga cgtggactat attttcatgg
    21781 gccaggtggt tcccgccggc tgcggccaga ttcccggacg tcaggccacc attctggcgg
    21841 gcgtgccgga atccgttccc tgcatcacgg tcaacaaggt ctgttcttcc ggcatcaaga
    21901 ccattgatct ggcctttcag atgattttgc tggggcgcgc ggaaatctgc atcgccggag
    21961 gccaggagag catgagcaac tgccccttca ccctgcccga catgcgctgg ggaatgcgca
    22021 tgggcctgcc caacggccgc gtggtggaca cgatggtgta cgacggcctg tgggacgctt
    22081 tctacaaccg ccacatggcc attcacggct ccgaagtggc agacgagttc ggcttttccc
    22141 gcgaggagca ggacgagtgg gctctcaatt cccagcaaaa cgccgtgaaa gcgatgaacg
    22201 agggaaaact ggacgacgag atcttcccgg tggaagtcaa acagggcaaa aaagtcatca
    22261 tcatcgataa agacgaagga ccgaggccgg aaacgacgct ggaagggctc gccaaacttc
    22321 cgccggtctt cggacactta agcaccgtca ccggcaaacc gggctcggtc acggcgggca
    22381 atgcccccgg cgtcaatgac ggcggcgacg tctgcctgct catgagccgg gaaaaggccg
    22441 atgaactggg ccttcagccc atcttcacca tcgtggatta cgccgaagtg tcgcagccga
    22501 ccaaggacat cgcgaccgtg ccggggctgg ccatcaaaaa gattctcgat caaaacgaca
    22561 tgacgcttga ccagatggac gtcatcgaaa tcaacgaagc ctttgccgcg gtggccctgg
    22621 tgagctgccg gtccattctg ggcatgacga aggaagagat gttcaaaaag gtcaacatca
    22681 acggcggcgc ggtggcctac ggccatccca tcggcgccac gggggcgcgc atcgccatga
    22741 cgctgggcta tgaactgaaa aggcgcggcg gcggctgggg cgtctgcggc atctgctcag
    22801 gccatgccca gggcgacgcc atgctcatcc gcgtggacaa atagatgatt ttgcagggaa
    22861 cgctcgcggg cgttccctgc aaagcttaca ggccgattct ttgttgcctg ttaataatta
    22921 ttcaatataa caatatatta acttttatgt tgcacattaa ctaacgttat gttattgtgc
    22981 gcacaaaggt taaaataaat gaaaaatcaa gtaatcgaag aactagaaag gtatgtccag
    23041 ggcacgctgg gcgttccttt atcgattcag ccgtggagtg gcggtcggaa cctgccgttt
    23101 tttttgaaag accagtatgc tttctttcag gcaaagattg cagacctgtc ctgcattttg
    23161 attgtcgccg gaggcgaaca ggaatcaacg cccgccatga tccgcaagca cgttgatctt
    23221 gtgcaaccca gggcaggcgg tgaggcgctc atttatgttc aaaagcagat gagctcctat
    23281 aatagaaaaa ggctgatcga tcagaaagtc agtttcattg ttcccggcaa tcagatgtat
    23341 ttgcttcccc ttggcgttga tctgcgtgag cgctttaaac gagcccggac acaggcgaaa
    23401 tcactgagcc cgtctgcgca ggtcgttttg ctgcatacgc tgctgaaaag tccgcagaga
    23461 attttagccc tcaatgattt ggctttgcag tatggttatt ccgccatgac aatgtcgcgg
    23521 gccttcagcg agatcgattc tctggggctt tttgaaattt ctatccgaaa acgcaaacgg
    23581 gaacggattt taaaaggaac aaaactagaa atctgggaga agacgctgga ctatctgcgc
    23641 agtccggtga tccggcgttt atatgccgat ccgtcgatta atttgcctga cggcgtctgc
    23701 gctgggcttt cggcccttgc ccgctactcg gcccttgcag aaccgggcaa caaggtcctg
    23761 gctgtcggaa acggcacagg aaagttgttg attcaaaaat acggaaggga ggcgctgtcc
    23821 cgaaaagatg ttcaggatta tgagatcgaa gtttggagct acgagcccgc tttatttgcg
    23881 atggatggcg tggtcgatcg cctctccttg tttctttcgc ttcgcgatga agaagacgag
    23941 cgcgtggaag cggcgctgga acaaatgatg agagaaatgc aatggtaaaa ggtctggacg
    24001 tgttccaaaa gcattttcag gattatgcgg atcgttacat tcttatcggc ggaaccgcct
    24061 gtgacctggc gctgaaagaa gccggcctgt cctttcgggc gacaaaagat ctggatatcg
    24121 ttttatgcgt cgaagccatt gaccggcctt ttgtgaaagt cttctgggcg tttatccggg
    24181 ccggagggta tcaaattcag gaaaaggcat ccggtgaaaa gcaattctat cgttttacaa
    24241 aacccgtgga taattcgttt ccgtttatgc tggagctgtt ttcacgaaaa ccggatacgt
    24301 tatccattga cgctgagggt catctgacgc cgatccctat cgatgacgaa gccgccagcc
    24361 tgtcggcaat tcttcttgac gatggatact acttcttcat cacacaaggg aaacgggaaa
    24421 acaatggtct gatggttatc ggggtggatc ggttgattcc cctcaaagca aaagcctggc
    24481 tcgatctgac acaaagaaag caaaaaggcg aagctatcga ttcaaaaaac attcgcaaac
    24541 acaaaaatga cgtgctccgt ctatacagaa ttgctgaaac caacactcaa gtactcttgc
    24601 ccgataacat tacccgcgat ttgcagacct tctttaaggc catcattgca gagccgacgg
    24661 atttggcggc actcggtctg gcatcaacga ctttggaggc agtgattgcc gatttgaaaa
    24721 aaatgtatgg aatgatctga tactgacaaa aagccattta tggattgccg caaaaacgtg
    24781 gtgtctgcgg catctgctcg ggccatgccc agggcgacgc catgctcatt cgcgtggaca
    24841 aatagatgat tatgcaggga acgctcgcga gcgttccctg ctgaatttta tccagcagtt
    24901 tgatgaagtt tctcttggct ttgtacctgg tccgaagctc gtccacgtaa gccgtaaacg
    24961 cttcatcctt gcgctgccga aacatcaagg gctgaatctt tttgatcaac tggacagcct
    25021 cttcatacgc cttattgttc gtctgctcga ttgtcggatt gacatatttc atgtacactt
    25081 ccacggcgtc cagcggatga tcctgcgcgc gcagcgccgc cagtttcatc cagagagact
    25141 gcgaacaccc gccttccagg gcttccttcc aggcggcctc cggttctttc tcccacagaa
    25201 aaatttcaac cagcagggaa taccccggat tccaatacca tcgattggtt tttcgatcct
    25261 gcttcgtaat gtgagaacga atgtgcgaga gagttttttc ccgccaatcg gaccactgtc
    25321 cggttttgtc cgcctgtttt tttaagcgct gatagctgtc caggccaagc tgatccgtaa
    25381 aatttgccca gatcaaattt aaggcttccg cggggcgctt tcggcgatga tattcatccg
    25441 ccaggaattc gcgcaggcgg gaatcggttc tggcgggaaa cgccttgaga cctgcttcgg
    25501 cccattccag cgccttatct ttattgccgg ctttcttgta aatttcagcg atttctaaat
    25561 agtggtacgc ataagttaaa tcccgttttt tgatgtccac cagctcttca atatcgccgg
    25621 atatcacggc cagcgtctct ttgattccga tgagattgag cgctgcattg gcattgctcc
    25681 ggcgaagttc ctgcttcgat gcggaaggcg cgaattcctt ccaagcctcg tcggccagct
    25741 gccgataaac cgccaggccc ttgtcgccca gaatggaagc gtaggttttc gcggcgccgg
    25801 aaaaaatatc ccattcgctg ttcagttcca gttcaaaaag atgcgccgcc agttttgccg
    25861 gatcctgctt ttctttttcg caggacttca gatgaagctc ctgcaagcga tacatgacgt
    25921 ctcccatgcc gccgtccgaa tcgtccatgt attgcaccgc ctcggccgcc tgctgaagtc
    25981 catactccgc aagctccgcc acgtccccgg catgcggtcc gtccagtaat tcctcgatgg
    26041 aatccaccat catcacccat cgggcaggtg caggagtaat cgatgtcatt tcccgcggca
    26101 ggtttcattt tgacggtgta agtccggctg ccgtctactt tggcgatgat ggttccgtta
    26161 tactcggcaa gatcgcgcac ctgaccgttt tcataataat ccctgccccg ctccagcgca
    26221 cggcgtggag caagggttgc gattattttt tcgttcaatg aatttttcat ttcgattcaa
    26281 acgggcctct tcaaaatctt tcgctgcgtc aaacccgtgc gatttttatt ttcttccgtc
    26341 agggcggcaa accatccgga agggagaaca gaggatttct cttaagttgg caatgccgga
    26401 tttcatgtta tcttcccgtc accggaatgg tatttttagc agcaatggtt tgatgccccg
    26461 tcaaacgtgg ccggcccagc gactatagga atgcaaaaaa ccggaatccg tacgtacccg
    26521 cacgaaaatg agtaattgcc gcgcagtttc aaacgttcaa acgttatctg ttcaaacgtt
    26581 atcttgacat acggactttg ccgtgtttta caaaagttgt tttgccttgt cccccataac
    26641 gcgacaaaca agatcgagaa tttgaaaatt aatgctgttt accctgttca ccatttttgc
    26701 ttcctcgttc gtcatcgcgc tttccggcgc gctgatgccg ggaccgctgt tgacggcgac
    26761 gatcagcgag agctcgcggc ggggtttctg ggccgggccg ctgatgattg ccggccacgc
    26821 cattttggag ctggcgctgg tggttgctct tttcctgggg ttggccccgt tttttcagat
    26881 gccggcggtt tttgcggc
//
LOCUS       DAOPAF010000020        25997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2055_length_25997_cov_48.345733, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000020 DAOPAF010000000
VERSION     DAOPAF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 25997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25997
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_25997_cov_48.345733"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PLQ77_03615"
     CDS             <1..1587
                     /locus_tag="PLQ77_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HPL09798.1"
                     /translation="TGGELSTTGAFALATDAKGEPSVKYAGQISVDHLATIDRARAHD
                     FLKWKQLRFQSLSAGYNPLFVNIREIALSDFFAKIVIHPGGATNIQHIFGVAKKETGR
                     PDAAAKAGEKIAAAKTPPPAVSAKPAQPPPDIKIGKVSFHGGTVDFADRNIRPNYAVT
                     MLNLQGSVTGLSSQQISRAKVDLKGNLGYGSPLEIAGTINPLAKDFFADIKLNFKDIE
                     MSPVTPYTSKYLGYPVTKGKLNFNVSYLVDQRKLSAENKIFFDQLTFGDRVESPEAIK
                     APVPLAVSLLTDRNGQINLDIPLSGSLDDPKFKIWPIVWQVLKNIITKAVTAPFSLLS
                     SLTGGGEEMSFVEFNFGGADIPEAELKKISALSKALYDRPQLKLEVEGYVDATQDQEA
                     LKKARFNRLLKTQKRKEMIDRGETPATLEEITIGQAEYEKYLTLAYKAAKFSKPRTAL
                     GIAKSLPPKEMEKLLFDNTIVTDADLEQLASRRAQAVREQLLKDGKVEAGRVFIVQPK
                     AKTPAKKEKVKDSRVDFKLK"
     gene            1620..2768
                     /locus_tag="PLQ77_03620"
     CDS             1620..2768
                     /locus_tag="PLQ77_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPL09799.1"
                     /translation="MIKLRAIMLLLLLGLLAGSCAPLLPVIVPVGKDQARCLPFFPDQ
                     DGWYGGDGAYSIALDKKRSLWLFGDTFVSKEKGRKDRIGMEVILGTTLAVSTCAGEQF
                     SIRYYLKKTNGKFASSFGPDQWLWPQDPFIADRVLYIPLLVIRPLPGPARPFHFEIAG
                     HTIARIRDYSAEDPNNWPVDYLDWTGAIAPGIEALAAASVVHGRFVYFYPLYRSGKDG
                     APVQGNLLARLPADHLENPAGHLEYWTLDGWQKKITPENLKMIFSPGLSELSVRYRMD
                     NEEWLAVYLSPEDRGRRLLYKTSRKPEGPWSPPAALIQSIDEVDPASPLYHPQTFCYA
                     GKEHPQFASGRDLVATYVCNSMENIDDQESFLRRNLFLYRPVVRLLRR"
     gene            complement(2793..3164)
                     /locus_tag="PLQ77_03625"
     CDS             complement(2793..3164)
                     /locus_tag="PLQ77_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL09800.1"
                     /translation="MKGNILVIEDNEQNLYLVRFILEKNGYTVHAAMDGQTGIDMAAA
                     LKPDMILLDIQLPVMDGYAVARNLRANPELAAIPIVAVTSHAMVGDRDKAMEVGCNGY
                     IEKPINPDTFMSEVERHLVVK"
     gene            complement(3161..4864)
                     /locus_tag="PLQ77_03630"
     CDS             complement(3161..4864)
                     /locus_tag="PLQ77_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPL09801.1"
                     /translation="MFKCHRKNAEGKAVIFFAHLMRWLFILTLVSFGCSSMALAESRV
                     VKIGVYENSPKVFTDESGRPSGIFVDIIKYIASKEDWELQYVAGTWAEGLERLEKGQI
                     DLMPDVALTAEREKKFSFHKIQVLSSWFQAYARKGSGLQSILDLNGKRVAILDQSVQQ
                     EAFTRFSRGFGLDTKLVAVADYKTMFEMVAKGEVDAAVANQFYGSMHARKYDLVDTAI
                     VFEPSALFFAAPKNQSQELLDAIDKHLDFLKKNTKSAYYATLKKWTSEEVRFKLPVWL
                     QILGLITGIVLLMSLGGSVILKYQVNARTRELQKINAEMEGRIIERTAELALAMEKAQ
                     SADRLKSAFLATMSHELRTPLNSIIGFTGILLQRLGGPLNDEQDKQLKMVYNSAKHLL
                     SLINDVLDISKIEAEQLNVVSETFSLRETVEKVIKTSQPLAENKNLKLTAEIAPEIGL
                     MKSDRRRVEQILLNLVSNAVKFTEQGFVRMKCEIQNGKIVIAVADSGIGIRPEDMDKL
                     FNAFQQIESGITRKYDGTGLGLSISRKLANLLGGEIRVESEWGKGSTFTFLLPREGEK
                     A"
     gene            complement(4876..5439)
                     /locus_tag="PLQ77_03635"
     CDS             complement(4876..5439)
                     /locus_tag="PLQ77_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPL09802.1"
                     /translation="MRTDLNFSYKFNEKYRAVSYVFVQANDEMSNYDYTEWGAGLQYQ
                     TPAAWLSFLAYYQQGYSKDDDGHWALEQKPSINLNLSKIFFNFKIFNQIRYEYRITPD
                     WNDARIKNTLNISRPDLLLQPYVGWELFYENRDKAVMLNRIKAGINKNIGAHVSVGPY
                     FRMDFSNVNSRWEWTRQLVGLQMTINY"
     gene            complement(5624..6556)
                     /locus_tag="PLQ77_03640"
     CDS             complement(5624..6556)
                     /locus_tag="PLQ77_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL09803.1"
                     /translation="MAFARPEILRPPSEHASYYLPLTSGCSNNSCAFCAFSFTSLGIR
                     DLDDVKREIDAMSLYVNSRMWMAGQPDIVYAILRQWDGKRVFLQDGDALVYPYPKLME
                     ALRYLNGKFPALERIASYATPQDILRRSVQELRDLKEQKLGILYMGVESGDDEVLKKI
                     RKNVTHDQMVEAAKKVKASGILLSVTVILGLGGVAGSEKHALESARILTEMDPDYAGA
                     LTLTLIPETELYKEWEQGRFEMITPFDSLRELKTMVEHSTFSNCFFSSMHASNYFSIR
                     GTMPKEKGRVLRQLQALLSRKDPGMLRPEFMRGL"
     gene            6746..8170
                     /locus_tag="PLQ77_03645"
     CDS             6746..8170
                     /locus_tag="PLQ77_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL09804.1"
                     /translation="MNLDALFYPKSVAVIGASDNLGGGKLPYFQILQANGFAGALYPV
                     NPRRAEVGGVKTCASIEDLPEPVDFAIVAAPVEQSVDIVKAAVRKQIKFIHFFTSGFG
                     ETGNLQLEQEVIREARRGHLRIVGPNCIGVQCFESKVSFRLTHLQEKPGSIAFLGQSG
                     GITSSFVAMATTRGIGLNKVVSYGNQIDLRLEDYLEYLADDDKIRLIACYVEDIKDVN
                     KFLKTLRRTTARKPVIILKGGKTELGSKAAASHTGAMASNYTIWAAAVRQHGGLLVDD
                     FEELMNLAMLGTAQRFPRGRRVGFMGAGGGISVLFSDLAVTSGVLLPELSPGTQRMIS
                     EKIMGVNTSTTNPVDLGAFGFDFNVMLHTMKALNEDVNIDVIVPYFSVEYIMHAEVFL
                     KVTNSADSIMAMAQQIKKPVIPVLSCFLENDLEAERVRISTFNALRKAGFPVYSTIQE
                     ALYVIETYFEWSEKRVRGSRKTAE"
     gene            complement(8226..8621)
                     /locus_tag="PLQ77_03650"
     CDS             complement(8226..8621)
                     /locus_tag="PLQ77_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPL09805.1"
                     /translation="MNHVHHVHLFASDIEKSLAFYQNAFGGKIILDEELAGARNVFMR
                     VGTGRIHFYDQAPSFSGRGSIHHFGIQTDDIDGVVEKLKAMGVPFKKSVTDIGVWKYV
                     MVPAPDNVLIELFQVNRDALPEAYRSYFD"
     gene            complement(8677..9510)
                     /locus_tag="PLQ77_03655"
     CDS             complement(8677..9510)
                     /locus_tag="PLQ77_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPL09806.1"
                     /translation="MTGIPEKIIDFHVHLFPDRLFDAIWEAFLKQYNWHIIHRCYYRE
                     CIERLRRAGVSVIVYSNYAHRAGIAEDLNRWNEKVLQEYEDLYCFAAYHPGDDHALDM
                     ARRLVENPKILGFKLQLLVQNFYPYDERLFDLYELVIRKNKRILFHTGTGPVGNPYVG
                     LDPFMKVLDRFPDLPATIAHLGAHEYAGFMELADRHPALYLDTAFCFLPAPYEGYNLG
                     PGYLEKYKSRILYGSDYPNLITPWEAEIENLLKMNLSSEFYDRIFHDNAAALIRSLTK
                     A"
     gene            9704..10042
                     /locus_tag="PLQ77_03660"
     CDS             9704..10042
                     /locus_tag="PLQ77_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09807.1"
                     /translation="MKRKWTALLAAMVICISAVPACAEVTQSDLVVLDVALIRPVGIV
                     SVAVGTALFIVSLPVSIPSGSVGAVAKSLVKDSFEYTFIRPVGNFDYELGTWGKEAPP
                     ISENSRSRIK"
     gene            10094..10357
                     /locus_tag="PLQ77_03665"
     CDS             10094..10357
                     /locus_tag="PLQ77_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09808.1"
                     /translation="MSTQLAVPLSQVALLLGISTLVLFFGRVKLALLINYCFTFYWGF
                     FLNPSFRSELGELVLNTYTYVYIGVGLLIVVLALIGFLSSNER"
     gene            10490..11449
                     /locus_tag="PLQ77_03670"
     CDS             10490..11449
                     /locus_tag="PLQ77_03670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPL09809.1"
                     /translation="MTYTWTEGHTKEKRGAKAMWTSITGFLRGALGLAFLGINTLFWA
                     VPVYITAVFKLILPFRAARNFFDVILNFLSYTWIYCINWFIGAIKTVEYDVTGLEKLK
                     RNEWYIVVSNHQTMIDIIVLQKIFHGRIPFLKYFMKQELIWVPILGLAWWALDYPFMK
                     RYSSELLKRKPHLRGKDVEATRRACAKFKDRPASIMNFVEGTRFSRAKQAAQKSPYRH
                     LLKPRSGGVSYVLSMMGENMRNLIDVTIVYPNGVEDAWRFFCSRISRILVRIEVLPID
                     PDLRGKNPDDQEYRNRFNRWLNDLWKKKDCTIEQTLARAFSRP"
     gene            complement(11466..11834)
                     /locus_tag="PLQ77_03675"
     CDS             complement(11466..11834)
                     /locus_tag="PLQ77_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09810.1"
                     /translation="MSEETHKKEHKSHDDQHLDKLTVKELRELAEQFPHEKAVHEMKK
                     EELVAFIKEAKGIKDEPHAHKHKPKGKIRMTKPEVKAKIRELKFLHGRALEDSDREKA
                     LLLRRQISRLKKISRRVAEA"
     gene            12063..12614
                     /locus_tag="PLQ77_03680"
     CDS             12063..12614
                     /locus_tag="PLQ77_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPL09811.1"
                     /translation="MKPTEILKEEHRLVLLMLKIMEAVCKKLEQNQSVKTEDLDAIID
                     FIRGFADGCHHAKEEKLLFPALEKTGMPQTDGPIAVMLREHALGRGFARSMSDAIGLM
                     KNGDATAAKTFVENARGYIQLLWNHIDKEDHVLFMMAEQRLSPAAQDELVEGFDRVER
                     EETGEGVHEKYHALLHRMRDDYL"
     gene            complement(12686..12761)
                     /locus_tag="PLQ77_03685"
     tRNA            complement(12686..12761)
                     /locus_tag="PLQ77_03685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12726..12728),aa:Phe,seq:gaa)
     gene            complement(12821..13453)
                     /locus_tag="PLQ77_03690"
     CDS             complement(12821..13453)
                     /locus_tag="PLQ77_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPL09812.1"
                     /translation="MTTYAFAGFWRRFIAYMIDGFIVSVVIIFLGVVAGVAYIAGAMS
                     GDGSAIVAQMTDPDKLALLTAWIWLLTFAVNIAYFTYFHGSTGRTPGKRLLGLQVVSV
                     TGESISFGTAFLRSVGYLVSSLVFCLGYLWVAFDRRKQGWHDKIAGTVVIIREPRGNE
                     SGISIPDSSSGPQSPAGFGCVPVNVSQPEENKPDAPSVQGASGVDGQKMP"
     gene            complement(13450..14223)
                     /locus_tag="PLQ77_03695"
     CDS             complement(13450..14223)
                     /locus_tag="PLQ77_03695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPL09813.1"
                     /translation="MTLPRIYSPETLQNASQCVLGADQLRYLKTVLRLKPGDAVLVFD
                     GYGHEFEARIENFSPSGVAIRLGKLLPPVKRKIRLTLAQGIPKAGKMDGIVKTAAELG
                     TDVIIPVAAARSIRRIEGEKSSAKVARWQKIVQEAARCTRNSYVTVVEPVLSFADMLK
                     RADSGARKLIFWEEEDRQTIRNVLQDDRFSGAAHYFIIVGPEGGLAREEVDRARESGF
                     VSVSLGRQILKVETAAAAILSIIQYEKGIFSSVAEGGGK"
     gene            complement(14272..15216)
                     /gene="prmA"
                     /locus_tag="PLQ77_03700"
     CDS             complement(14272..15216)
                     /gene="prmA"
                     /locus_tag="PLQ77_03700"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPL09814.1"
                     /translation="MTQKEPEKWLKIQISASAEQIDALSNFLEETGAQGVFSESLMPP
                     GADDFPESSGLEVINAFFPADVRAEKRMKAVQKYLKSLEEIFPDAAVPVLSSETITDP
                     DWGEQWKKYFKPIRVCQNIVVKPTWERYAPDSRDIVVEIDPGMAFGTGQHASTRMCME
                     AIEDIILKDRSTGEWKVLDAGCGTGILGITAAKMGAQDVICVDNDPRATEIAAQNTVI
                     NAVEDRVQILNQDAARISRPRNLIIANLTAKLLLKLRPQLVRLLLPGGYLIISGIIEN
                     DVPAMEEHFIVAPLVQHRRLTEKEWVCCVLRKENGGPS"
     gene            15449..16135
                     /locus_tag="PLQ77_03705"
     CDS             15449..16135
                     /locus_tag="PLQ77_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPL09815.1"
                     /translation="MLRFEELILSGKIKIGEKLPPERELALQLGVSRPVVHEGLVELA
                     SRGLVSIKPRFGATVNDYRKEGSIALMASLIQYQKGNLEPELLESLLQIRMMLEPPFA
                     RLAARNRTSEQILELHGILEKEAAANGRNPQALTDLDFEFHLLIALASGNRVYPLLLN
                     SFRSVYTNLSGQFFRNTKVTDAVHGYHEKMVNAVESGNETRAASVMKAILNHGEKHLR
                     EMIGQESLSL"
     gene            16158..18668
                     /locus_tag="PLQ77_03710"
     CDS             16158..18668
                     /locus_tag="PLQ77_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPL09816.1"
                     /translation="MKTALANEVHLKEPKALSGRIQWLRDYYFRGTERPWNNEFTCWT
                     TGTPWDVIYNELTFYIVPETYTLLNTLGASYLQAARPVALHRDFWTWPLVERRAWFVK
                     EAIVNYVPQEILPGDLLAGARFNIQTSMCFTKEESRAWQKLVKGKNGARAKMKWFHDH
                     GYGNAGATSGHLIPGHERLLQIGWKGVHAELMSRYNALSSAERAASKGAQLRAMMTAS
                     TMARDLAGRYRSLCEDLAQKETDAVRAAELRTMAANLARVPWEPPTNFWEAVQALWIN
                     HMLVMTDENYPGAGVSFGRADQYLYPYYEKSLRDGMQRDWTKEILKCFWVHANTAYDA
                     MIRNGNQGITAGFGQLITLSGLGADGRDMTNDLTYAILEVIDEMSPILEPKPNVRLHR
                     NSPEELYDKIVDMIESSQGAPFLLNFDERSMAGMMLEARKAGLAHLIHEGNVHEYAPV
                     GCLENTMVGNDRSGTVDNNLNLLKAVELALTGGKDLLPFYDPMTGKTEAVRQDGPRTP
                     DAASIQTWEEFWEAYAAQTRYIIQKCVDLYETSESIRARFFPTPYLSCMVEGCAEQGL
                     DVTQGGAQISFTTLEAVTYATTVDSLLAVKYLVFDKKVCTLPQLADALRANWKGHEVL
                     QAMAKNRAPKYGRDDDEADELALRVMQLWCAETWRHRTQSTGRRYRPGMLSWNYWAGD
                     GYIMAASADGRTKGQFLSNAICPSNGADIKGPTSNANSVGKVLGGKAEDGRGDWLDYL
                     NVLPNGASHTITFNPSILRDPVHKDKFKAFLKGYTQNGGTALQINMLDSGMLKDAQDH
                     PENYRHLLVRITGYNAYFTTVGRELQNEVIERVSHQQF"
     gene            18681..19709
                     /locus_tag="PLQ77_03715"
     CDS             18681..19709
                     /locus_tag="PLQ77_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPL09817.1"
                     /translation="MTSSKGKNGLVLQILRMSTEDGPGIRTSVFFKGCPLGCLWCHNP
                     ESISPKPQVQWLHTSCIGCGNCVEICPEKALEKTTDGIMINRRLCTGCGLCAEECPTT
                     AMELLGKKWSVSDLVHELVKDRVYFEQSGGGVTLTGGEAALQHEFCLPLLKELRAVGI
                     STALDTCGQAPQKTLASLLPYIDVLLYDLKEIDPEKHKGFTGAGNAKILENAVFAARF
                     KKDHPYPRTLWIRTPVIPNSTDTPENIAGIGRFIHENLEGAVDRWELCSFNNLCRDKY
                     KRLGLNWPFAEAELMKKTVMENLAGVAKSAAPKTNVCWSGSTTLEKRADSQEPPEKQN
                     KRKPVCAG"
     gene            19733..20578
                     /locus_tag="PLQ77_03720"
     CDS             19733..20578
                     /locus_tag="PLQ77_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09818.1"
                     /translation="MTIKNRFSESDMKAFAPSEKVGIIATVTPEGLPHLSLLTSVMAS
                     TPTQLTIGEFSTGVSKAYLQQTRKTGFAIVTLDKKLWRGKALWTHLKKEGPEYEVYNQ
                     QPMFRYNTYFGINTVHYLELKETTEGSPLPLVAVAASAILTRLAKGAAAQSAKVKALS
                     PFGKNLFDRLDSLKFLSFIDDDGFPTVIPVIQCQACDENRLAFYPGVFAEELNRLEAG
                     KTMAIFGMTMEMEDVLVRGVFQGYARRRGIRLGTLDIDWVYNSMPPNHGQIHPPLPLK
                     AVTDF"
     gene            complement(20604..21089)
                     /locus_tag="PLQ77_03725"
     CDS             complement(20604..21089)
                     /locus_tag="PLQ77_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPL09819.1"
                     /translation="MMVVWVLLGIMVCAGSAFAANETGRDGRFVAYDDGTVLDTTTNL
                     MWADKDNGVDIQWIAAKTFCENFSTGGYDDWRMPTKNELAGLYSGSSRDAHGYHLATS
                     LISLTGCCPWTSDTCISMLPKYEMAGYYAFNLSFITGQSVCSQQGLDSDVRVLPVRFH
                     R"
     gene            complement(21130..21711)
                     /locus_tag="PLQ77_03730"
     CDS             complement(21130..21711)
                     /locus_tag="PLQ77_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL09820.1"
                     /translation="MNIKDFRQQENGVHEIFLHRWSPRAMSGADLDEAALLNLFEAAR
                     WAPSANNNQPWRFIYARRDTPRWEVFFELLAEGNRIWAKNAAVLIAVISKTTFDSGSP
                     ARTHSYDAGAAWVSLALQGSLKNLVVHCMQGFDYDKAKEALRVPDDYAVEAMIAVGRP
                     GNKEDLPEYLQEREFPSPRKKLDEIVMEGVFQK"
     gene            21879..21954
                     /locus_tag="PLQ77_03735"
     tRNA            21879..21954
                     /locus_tag="PLQ77_03735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21913..21915,aa:Glu,seq:ttc)
     gene            complement(21971..22978)
                     /locus_tag="PLQ77_03740"
     CDS             complement(21971..22978)
                     /locus_tag="PLQ77_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPL09821.1"
                     /translation="MSRPLRIQYPDAFYHVTCRGNERREIFRHADDRKEFFRLLSRSL
                     TIFEVQLLAYAMMPNHFHLLVCTPKGNLSEFMRHFNISYTGFFNRKHKRTGHLYQGRY
                     KAFLIDENNYLMEVSRYIHLNPLRIKSQKPVGQRFRDLMINDSTSFPGYLQPQNRKSF
                     VQYKIILDYFGGENHNAMNEYQKFVVEGMERDLPSPLEKGKGTGIVGGEDFIEQIQKT
                     FSKSKGHQRSSREQPALRKLGKQIKPDHLIDRYARLVKISREDLTRKGKQSTQRAMLM
                     EILYRICNLTQPEIGSLLGGVDYSAVSQARKRLQIKIENNPELGGELIKIQNELVKCQ
                     E"
     gene            complement(23083..24168)
                     /locus_tag="PLQ77_03745"
     CDS             complement(23083..24168)
                     /locus_tag="PLQ77_03745"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HPL09822.1"
                     /translation="MFKKAVFPGKYIQGAGALKELPALISLFGARALVLASPTAAKTI
                     VPVCGIHEETNQAFIETFGRECCEKELVRLAKVIEKNRIGVMIGMGGGKTIDTAKIAA
                     DRANIPVIIVPTIASTDAPCSGCAVVYSENGVHEREYYQKSNPAAVVVDTGIIAAAPV
                     RFLVAGMGDALATWFEAESCRRTQSKNECGGLSTMAGLHLARLCYDTLLACGAAAKLA
                     CEHQVPTPALERIVEANILLSGIGFESSGLAAAHAIHNGLTALEETNAYYHGEKVAFG
                     VLTGLHLTGASPEVMRTVYGFCREMGLPVTLADIGLDKRDRIGLRKAAEKTCAPGAAV
                     HHEAGDITPERVLHAMIAADAAGQKMR"
     gene            24412..25089
                     /locus_tag="PLQ77_03750"
     CDS             24412..25089
                     /locus_tag="PLQ77_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPL09823.1"
                     /translation="MIMHGADNARFWTDQLFNAMITADRREASGVMDRMMAAGVNSQS
                     IIDDVLDPTLIRLGDLWEQDAVSLTQTFVASKIAEDVLLRCVSASGKTAKRGAPVVIG
                     NIEDDFHSLGRRIVGSFLRAGGWEVHDLGNDVTAEQFVDKAIEVGASVVGASAMMHTT
                     ALNIRKLRDLIDARGLNHQLKLAVGGAVFNWRPDLVAEVGGDGTARNATGVDALFQRL
                     RDAAKGG"
     gene            25116..25988
                     /locus_tag="PLQ77_03755"
     CDS             25116..25988
                     /locus_tag="PLQ77_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPL09824.1"
                     /translation="MNTLQGLPTDRVPVFAVLGAYGGKLAETGLKTLYSDADAYVSGQ
                     QAVQNALGFDMILATFDYSAIAEAFGGEVAWFDNQTPNMKRPAVSEASAVAGLKLPDP
                     RRDARLPVILSATRQLVSLYRESVPVFAAVPGPGALPALVLGLETWMETVLFDEDNAR
                     RILERTGRFFLDWVHALCEAGVTGLVVTESVASVEISTRDLFAKVFLPHLHDLFGQIN
                     APLVFHHGGGRILHILDLLPGLPGLAGVVVGSKDDLGAARSALGTELTLIGNLDNLSL
                     PAATFCRTPPRMCR"
BASE COUNT         5886 a         7403 c         6981 g         5727 t
ORIGIN      
        1 accggcggcg agttgtccac gaccggagcc ttcgcgctgg ccaccgacgc gaaaggcgaa
       61 cccagcgtca aatatgccgg ccagatctcc gtggatcatc tcgcgacgat cgatcgggcg
      121 cgcgctcacg attttctgaa atggaaacag ctccgttttc aatctctttc cgccggctat
      181 aatcccctgt tcgtgaacat tcgggaaatt gcgctcagcg atttcttcgc caaaatcgtc
      241 atccacccgg gcggcgccac caatattcag cacatcttcg gcgtggcgaa aaaggaaacc
      301 ggaaggcctg atgccgcggc gaaggccgga gaaaagatcg cggccgccaa gaccccgccg
      361 cccgctgtga gcgcaaaacc ggcgcagccc ccgccggaca tcaaaatcgg aaaggtcagc
      421 tttcacgggg gcacggtgga tttcgccgac cgcaacatcc ggccgaacta cgccgtgacc
      481 atgctcaatc ttcagggaag cgtcacgggc ctctcctcgc agcagatctc gcgtgcgaaa
      541 gtcgatttga agggaaacct gggctacgga tcgccgctgg aaatcgcggg caccatcaac
      601 ccgctggcca aagatttctt cgccgatatc aagctcaact tcaaagacat tgaaatgagc
      661 ccggtcacgc cttacaccag caaatacctg gggtatcccg tcaccaaggg caaactcaat
      721 ttcaacgtgt cctatctcgt cgatcagaga aagctgtccg cggaaaataa aatctttttc
      781 gatcagctca ccttcggcga cagggtggaa agccccgagg ccatcaaggc gccggtgccg
      841 ctggccgttt cccttttgac cgaccggaac ggacagatca acctcgacat tcccctgtcc
      901 ggaagcctcg acgacccgaa gttcaaaatc tggcccattg tctggcaggt tctcaaaaac
      961 atcattacca aagccgtcac ggcgcccttt tctcttttgt cttcgctgac cggagggggc
     1021 gaggaaatga gctttgtcga attcaacttc ggcggcgcgg acattccgga ggcggagctg
     1081 aaaaagatca gcgcgctttc caaggcgctg tatgaccgtc cccaactgaa gctggaggtt
     1141 gaaggctatg ttgacgcaac acaggatcag gaagctttga aaaaagcaag attcaaccgt
     1201 ctgctcaaaa cgcagaagcg caaggaaatg atcgaccgcg gcgagactcc cgctacgctc
     1261 gaagaaatta caatcgggca ggccgaatac gagaaatacc tgaccctggc ctacaaggct
     1321 gcaaaatttt ccaagccgcg cacggcgctg ggcatcgcca aaagccttcc gcccaaagaa
     1381 atggaaaaac tgctcttcga caataccatc gtcacggacg ccgatctgga gcagcttgcc
     1441 tccaggcggg cacaggcagt ccgcgaacag ctcttaaaag acggcaaggt ggaagcgggg
     1501 cgcgttttca tcgtccagcc caaagccaaa acgcccgcga agaaagaaaa ggtaaaagac
     1561 agccgtgtgg actttaaact gaaatagcgg ttgcgcggcg attttcggat tcggaaaaca
     1621 tgataaaatt gcgcgcgata atgctccttc tgctcctcgg cctgctggcg ggctcctgtg
     1681 cgccccttct gccggttatc gttcccgtcg ggaaagacca ggcccgatgc ctgcccttct
     1741 tccccgatca ggacggctgg tacggaggcg acggcgccta ctccattgcc ctggacaaaa
     1801 agcgctccct gtggctgttc ggcgacacct tcgtttcgaa agaaaagggg cggaaggacc
     1861 ggatcggcat ggaggtgatt ctgggaacaa cgctggccgt ttccacctgc gccggcgaac
     1921 aattttccat ccggtattat ctgaagaaaa caaacggcaa attcgcatcg tccttcggcc
     1981 cagatcaatg gctctggccg caggacccgt tcatcgccga ccgggtttta tacataccgc
     2041 ttctggtgat taggcctctt cccggaccgg caaggccctt tcactttgaa attgccgggc
     2101 acaccatcgc ccggatcagg gattattcgg cggaagatcc caacaactgg ccggtggatt
     2161 atctggactg gaccggcgcg atcgctcccg gcatcgaggc cctcgccgcc gcatccgtcg
     2221 ttcacggccg tttcgtctat ttttatccac tgtatcgttc cggaaaggac ggcgctcccg
     2281 tccagggcaa tcttttggca agacttcccg ccgaccatct ggaaaatccg gccggccacc
     2341 tggagtactg gacgctcgac ggctggcaaa agaaaatcac tccggaaaac ctgaaaatga
     2401 ttttttcccc cggtctttcc gaactgagcg ttcgctatcg catggataat gaagaatggc
     2461 tggccgttta cctgtcgccg gaagacagag gccgccgcct gctgtacaaa acatcccgaa
     2521 agccggaagg cccgtggagc ccgcctgccg cgcttatcca atccattgat gaagtggacc
     2581 ccgcaagccc gctgtaccat ccgcagacgt tctgctacgc cggcaaggaa cacccccagt
     2641 tcgcaagcgg gagggacctc gtcgcaacgt acgtctgcaa ttcaatggaa aacatcgatg
     2701 atcaggaaag ttttcttcgc cggaatcttt ttttgtaccg gccggtcgtc cggctgctca
     2761 ggcgctgacc atgaacggcg tcagtccgcc tgttatttga ccaccagatg gcgctccact
     2821 tccgacatga acgtgtccgg attgatgggc ttttcgatgt agccgttgca gccgacctcc
     2881 atggctttgt cgcggtcgcc gaccatggcg tgcgacgtca cggcaacaat gggaatggcg
     2941 gcaagctcag gattggcgcg cagattccgg gcgacggcgt atccgtccat gacgggaagc
     3001 tgaatgtcga gcagaatcat atccggtttc aacgcggccg ccatgtcgat gcccgtctgc
     3061 ccgtccatcg cggcatgcac cgtatagccg tttttctcca gaataaaccg gaccagataa
     3121 aggttttgtt cattgtcttc aatgaccagg atgtttcctt tcatgctttt tctccctccc
     3181 gcggcaacag gaatgtgaag gtgctgcctt tgccccattc gctttccact cggatttcac
     3241 cacccagcaa attcgccagc ttccggctga tggacaggcc caatcccgtt ccgtcgtact
     3301 tgcgcgttat gccggattca atctgctgaa acgcgttgaa aagcttgtcc atgtcttccg
     3361 gccgaatgcc gattccggaa tcggcgacgg cgatcacaat tttcccattt tggatttcgc
     3421 atttcatccg gacaaatccc tgctcggtga acttgacggc gttgctcacc agattgagaa
     3481 gaatctgctc cactcgacgg cggtcgcttt tcatcaggcc gatttccggc gcgatttccg
     3541 ctgtcagttt cagattcttg ttttccgcca gcggctgaga ggttttaata actttctcca
     3601 cggtctcccg cagactgaag gtttcagaga ccacgttgag ctgttccgct tcaatcttgg
     3661 aaatatccag aacatcgttg atcaagctca gcaaatgctt cgcgctgttg taaaccatct
     3721 tgagctgctt gtcctgttca tcgtttaacg gaccgcccag gcgctgcaga agaataccgg
     3781 taaagccgat gatggaattg agcggcgtgc gcagttcatg cgacatggtc gccagaaaag
     3841 cggatttcag gcgatccgcc gattgcgctt tttccatggc gagggccaac tcggccgtcc
     3901 gctcgataat ccgcccttcc atttccgcat tgattttttg caactccctg gtgcgcgcat
     3961 tgacctgata cttcaggata acgctgcccc ccagactcat cagcagcaca ataccggtaa
     4021 tcaaaccgag aatttgcagc cacaccggca gcttgaaacg cacctcttcg gatgtccatt
     4081 ttttcagggt tgcataatac gctgattttg tgtttttttt caggaaatca agatgtttgt
     4141 caatggcgtc cagcaattcc tgggattgat ttttcggggc ggcgaaaaaa agcgcggacg
     4201 gttcaaaaac aattgccgta tcgaccagat catacttccg ggcatgcata gagccgtaaa
     4261 attgattggc aacggcagcg tcaacctctc ctttggccac catttcaaac atggttttat
     4321 aatccgcaac ggcaacaagc ttcgtatcca atccaaatcc ccggctgaaa cgggtgaaag
     4381 cctcctgctg aaccgattga tccagaatcg cgacacgttt gccgttcaaa tccaatatgg
     4441 attgtaatcc gctgcctttg cgggcgtagg cctgaaacca ggaggacagc acctgaattt
     4501 tgtgaaagga gaatttcttt tcccgctcag ccgtcagggc cacatccggc atcaaatcaa
     4561 tctggccttt ctccaggcgc tccagtccct cggcccaggt gcccgccaca tattgcagtt
     4621 cccagtcttc ttttgacgcg atatatttga taatatcaac aaagattccg gaaggccggc
     4681 cggattcgtc cgtgaaaacc ttgggcgagt tttcataaac cccgattttt accacccggc
     4741 tttcggccag agccatggaa ctgcacccga atgaaaccag cgtcagaata aacaaccagc
     4801 gcatcaggtg cgcgaaaaag atgacggctt tcccttcagc atttttccgg tgacatttga
     4861 acataacatt tcttcctaat agtttatggt catctgtaat ccgacgagct gtctggtcca
     4921 ttcccaccga ctgttcacat ttgaaaaatc cattctgaaa taaggtccta cggaaacatg
     4981 cgccccgata tttttgttga tgcccgcctt tattctgttg agcatcaccg ccttatcgcg
     5041 gttttcgtaa aacagctccc atccgacata gggctgcaac aaaagatcgg ggcgggagat
     5101 attcagtgta ttcttgattc tggcgtcatt ccagtccggc gtaattctgt attcatagcg
     5161 aatctgattg aatatcttga aattaaaaaa aattttggac aggttcagat tgatgcttgg
     5221 tttttgctcc agagcccagt gcccgtcgtc atccttggaa taaccctgct gataataggc
     5281 cagaaaggaa agccaggcag ccggcgtctg atactggaga cctgcgcccc attccgtata
     5341 gtcgtaattg gacatctcat cgttggcctg gacgaagaca taggagacgg cgcggtattt
     5401 ttcattgaat ttgtaggaaa aatttaaatc cgtacgcaaa gatgtgtcat ggcccgcata
     5461 aagaagcgaa gcagacatca acatgagcaa ggatgcagca agaacaatat cgaagcacaa
     5521 aaaacatatt cttcgcatca tcaaccctgt cccttgactg ttcttggcgc acggactgga
     5581 tgtcttcagc aaaatttcat catcgttcca ctgggcaatc tttttacagc ccccgcataa
     5641 attccgggcg aagcatgccc gggtctttcc gggacaagag cgcctgaagc tgccggagca
     5701 ccctgccttt ttccttcggc attgtgccgc ggatggaaaa ataattggaa gcgtgcatgg
     5761 agctgaaaaa acagttggaa aaggttgaat gctccaccat cgttttcaat tcccgcagcg
     5821 aatcaaaagg ggtgatcatt tcaaagcggc cctgctccca ttccttgtaa agttccgttt
     5881 cgggaatcag cgtgagcgtc agcgcgccgg cgtaatccgg gtccatttcc gtcagaattc
     5941 tcgccgactc cagcgcgtgc ttctcgcttc ccgcgacgcc gcccagcccg aggatcaccg
     6001 tgacggacag gagaatgccg gatgccttca ctttttttgc ggcttccacc atctgatcgt
     6061 gggtgacgtt ttttcgaatc ttcttcaaga cctcgtcgtc gccgctttcc acccccatgt
     6121 aaagaatgcc gagcttctgt tccttcaggt cccgaagttc ctgaacgctt cgccgtaaaa
     6181 tgtcctgcgg agtggcgtag ctggcaatgc gctccaaagc gggaaatttg ccgttgagat
     6241 accgcagcgc ctccatgagc ttcgggtagg gatacacaag agcgtcgccg tcctggagaa
     6301 acacccgctt tccgtcccac tggcggagaa ttgcatagac gatgtccggc tggcccgcca
     6361 tccacatgcg gctgttcaca tacagagaca tggcgtcgat ttcgcgtttt acgtcgtcga
     6421 ggtcgcgaat gccgaggctg gtaaaggaaa aagcgcaaaa cgcacaggaa ttgttggaac
     6481 atccggacgt caggggcaga taataactgg cgtgctcgct gggcggtcga aggatttccg
     6541 gtcttgcaaa agccataatg ggctcccttc cataaatatt gtgataccat acagaccgag
     6601 ccgcccgttt gtcaagaccg tcctgacagg ttcgtttttt tcttgcatcg ttgcttcaaa
     6661 gttgtaagat agcgccgttt tgaaaccggc tttttgcgaa tacaaatcct tcgccacggc
     6721 cggatataga tcaaggagga atttcatgaa tctcgatgcg ttgttttatc ccaaaagcgt
     6781 cgccgttatc ggcgcatccg acaatctggg cggaggaaaa ctgccctatt ttcagatcct
     6841 gcaagccaac ggttttgccg gcgcccttta tcccgtgaat ccccgacggg ccgaagtggg
     6901 cggcgtcaag acctgcgcgt ccatcgaaga tcttccggaa cccgtcgatt ttgccatcgt
     6961 cgccgcaccc gtcgagcaat ccgtagacat cgtcaaagcg gccgtccgca agcagatcaa
     7021 gtttatccat ttcttcacct ccggtttcgg agaaacgggc aatctgcaac tggagcagga
     7081 agtcatccgg gaagcccgca ggggacatct ccggatcgtc ggccccaact gcatcggcgt
     7141 tcaatgcttc gagtcgaaag tttctttccg actgacccat ctgcaggaga agcccggttc
     7201 gatcgccttt ttggggcagt ccggcggaat cacctccagc tttgtggcca tggcaaccac
     7261 ccggggcatc gggctcaata aagtggtctc ctacggaaat cagatcgatc tgagactgga
     7321 ggattatctg gagtatctgg cggatgacga caagatccgt ctgatcgctt gctacgtgga
     7381 agacatcaag gatgtgaaca aatttctgaa aacgctgagg cgcacgacgg ccagaaagcc
     7441 ggtgattatt cttaaagggg gaaaaacgga gctgggctcc aaggcggcgg ccagccacac
     7501 gggcgccatg gccagcaatt acacgatttg ggccgcggcc gtgcggcagc acggcgggct
     7561 gctggtggat gatttcgaag agctgatgaa tctggcgatg ctcggcaccg cgcagcgatt
     7621 cccgcgcgga aggcgcgtgg gattcatggg cgccggcggc ggcatctccg tgctcttttc
     7681 cgatctggcc gtcacctcgg gggttttgct tcccgaactg agccccggca cccagcgaat
     7741 gatcagtgaa aaaatcatgg gggtcaacac ctcgacgacc aatcccgtcg atctcggagc
     7801 cttcggcttt gacttcaacg tgatgctgca caccatgaaa gccctgaacg aagacgtcaa
     7861 catcgatgtg atcgttccct atttttccgt tgaatacatc atgcacgcgg aagtgtttct
     7921 caaggtgacc aacagcgcgg acagcatcat ggcgatggcc caacagatca aaaagccggt
     7981 gatccccgtc ctgtcgtgct ttctggaaaa tgacctggag gcggaacgcg tcagaatttc
     8041 caccttcaac gcgctgcgca aggcgggatt ccccgtttat tccaccatcc aggaggctct
     8101 ttacgtcatc gaaacctatt tcgaatggtc ggaaaagcgt gtgcggggga gcaggaaaac
     8161 cgccgaatag cgaaaagata cggtaagaaa acgagggccc cggaatatcc gccgtcccgg
     8221 acggttcagt caaaatagct tctgtaggct tccggaaggg catcgcggtt tacctggaac
     8281 aattcaatga gcacattgtc gggcgcgggc accatgacat atttccagac gccgatgtcc
     8341 gtgacgcttt ttttgaacgg cacgcccatg gccttgagct tttcgaccac gccgtcgatg
     8401 tcgtcggtct ggatgccgaa atggtgaatg cttccccggc cggaaaagga aggcgcctga
     8461 tcgtagaaat gaatccgtcc ggtaccgacc ctcatgaaca cgttcctcgc tcccgccaac
     8521 tcctcgtcga gaatgatttt cccgccaaag gcgttttgat aaaaggccag gcttttttcg
     8581 atatccgacg caaacaaatg aacatggtgg acatggttca tggacttgct cctttcttta
     8641 cgggcgtcgc gcaggcaaac ggggggatgc cgccgcctat gctttcgtca gggaacggat
     8701 cagcgcggct gcgttgtcgt gaaagatccg atcgtaaaat tccgaagaaa gattcatctt
     8761 caaaagattt tcaatctccg cttcccacgg cgtgatcagg ttgggataat ccgacccgta
     8821 taaaattctg gatttgtatt tttcaagata cccgggtccg aggttgtacc cttcgtacgg
     8881 cgcgggcaaa aaacagaagg cggtgtcgag atacaaagcc ggatgccggt ccgcaagctc
     8941 catgaaaccc gcgtattcat gagcgcccag atgggcgatg gtggcgggaa ggtcaggaaa
     9001 acggtccaga actttcataa acgggtcaag gccgacatag ggatttccca cggggccggt
     9061 cccggtatgg aagagaatgc gcttgttctt ccggatcacc agctcgtaca ggtcaaagag
     9121 cctctcgtcg tacggataaa aattctggac cagaagctga agcttgaatc cgaggatttt
     9181 cgggttttcc acaaggcggc gggccatgtc cagagcgtgg tcgtcgcccg gatgataggc
     9241 ggcaaagcaa tacaaatcct cgtactcctg aaggactttc tcattccacc ggttcagatc
     9301 ctcggcgatt ccggcgcggt gcgcgtaatt ggaatacacg atcacgctca cgcccgcccg
     9361 ccgaaggcgc tcgatgcact cccggtaata acagcgatga ataatgtgcc agttgtactg
     9421 cttcagaaaa gcttcccaga tggcatcgaa aagcctgtcc ggaaacagat gaacgtgaaa
     9481 atcaattatt ttctctggaa ttcccgtcat ggtcgccacc gcccgcgcgt ctgtatgcgc
     9541 ctgtttggtt agcacaaccg gaattcataa tcaacggaaa ggtcggcggg tgcaaagatg
     9601 cccttccccg gcgcgtacgg gcggcgggga tttcttgacc cggcggaaaa tgtgggctaa
     9661 agaaaacggc aaaaccctta aaccgctttt ccgggggaat gtgatgaaaa gaaaatggac
     9721 cgccctgctt gccgcgatgg tcatctgcat cagcgccgtg cccgcgtgcg ccgaagtgac
     9781 gcagtccgac cttgtggtcc tcgacgtggc gctgatccgg cctgtgggca tcgtctccgt
     9841 cgcagtcggc acggcactgt tcattgtgtc gcttcccgtg tccattccca gcggcagcgt
     9901 cggcgccgtg gcgaaaagcc tcgtgaagga cagcttcgaa tacacgttca tcaggcccgt
     9961 cgggaatttc gactatgagc tgggcacatg gggcaaagag gccccgccca tctcagaaaa
    10021 ttcccgcagc cggataaaat aggcgctgga caagaaataa ctgctccggt ataaagaagc
    10081 catggaaatc ctcatgagca cacaactggc cgtgcccctt tcccaggtgg ccctgcttct
    10141 ggggatcagc accctggtcc tgttttttgg acgggtgaag ctggccctgc tcatcaacta
    10201 ctgcttcact ttttactggg ggttttttct gaatcccagc ttccgctccg aactgggaga
    10261 actggttctc aacacctaca cctacgtcta tattggggtc ggcctgctca tcgtcgttct
    10321 ggccctgatc ggatttcttt ccagcaacga acgctgagcc atccttttat ttcattcctg
    10381 tgtgcaagcg aagcgcgact ttgcgcctgc cccggaccgg gttcgggtgt cgcagaccag
    10441 gggtgttttc gaaaaaaaag ccgctttgca tttgattttt tccccgcaaa tgacctatac
    10501 atggacggaa ggtcacacca aggaaaaacg aggagcgaaa gcgatgtgga catccatcac
    10561 cggatttttg cgcggtgccc ttggtctcgc ctttctgggc atcaataccc tcttctgggc
    10621 cgttccggtt tacatcacgg ccgttttcaa gctgatcctg ccctttcgcg cggccaggaa
    10681 cttctttgac gtcattctca attttctatc ctatacctgg atctactgca tcaactggtt
    10741 tatcggggcc atcaaaaccg tggaatatga cgtcacgggg cttgaaaaac tgaagcggaa
    10801 cgaatggtac atcgtcgttt ccaaccacca gaccatgatc gacatcatcg ttcttcagaa
    10861 aatatttcac ggccgcatcc ccttcctgaa atatttcatg aagcaggagc tgatctgggt
    10921 gcctattttg gggctggcct ggtgggcgct ggattatcct tttatgaagc ggtactcttc
    10981 ggaactcctg aaaagaaaac cgcatcttcg gggcaaagat gtcgaggcaa cccggagagc
    11041 ctgcgcgaag ttcaaggacc ggcccgcatc catcatgaac tttgttgaag gaacccgctt
    11101 ttcccgcgcg aaacaagcgg cgcagaaatc gccctaccgg caccttctca agccgcggtc
    11161 gggcggcgta tcgtatgttc tttccatgat gggcgaaaac atgcgaaacc tgatcgacgt
    11221 caccatcgtt taccccaacg gcgtggaaga tgcctggcgg tttttctgca gcagaatatc
    11281 aagaatcctc gtccggatcg aagtgctgcc gatcgatccc gaccttcgcg ggaagaatcc
    11341 cgacgatcag gaatacagaa accggttcaa ccgctggctg aacgacctgt ggaaaaagaa
    11401 agactgcacc atcgagcaga cgctggcgcg cgctttttct cgcccctgac gcccggccgt
    11461 cgggcttacg cctcggcaac ccgccgggat atttttttaa gccggctgat ctggcggcgc
    11521 agcagcaagg ctttctcccg gtcgctgtcc tccagcgccc ggccgtgcaa aaatttcaac
    11581 tcgcggattt tcgccttgac ttccggtttg gtcatcctga ttttgccctt gggcttgtgt
    11641 ttgtgcgcgt gcggctcgtc cttgatgccc ttggcctctt tgataaaggc caccagctct
    11701 tccttcttca tttcgtgaac ggccttttca tgcgggaact gctcggccaa ttcccgcagt
    11761 tccttcacgg tcagtttgtc caggtgctga tcatcatggg atttgtgctc ttttttgtga
    11821 gtttcttcgg acatcgctca tagacctcct tgtttgattt caggaacatc aacgtgcaat
    11881 gcgaaaaagg gaaaccggtt tggatctcct ataccacaac cgcgcttgag gttcaacccg
    11941 cgcagccgat tccttccgat tgacacgaca ttgcatttgc cttattctct gcgcaaacaa
    12001 aatgccgaag ggccgcgcgc aaaactgttg gcttcagatt ttttttcaag gaggaggaac
    12061 tcatgaaacc gaccgaaatt ttgaaagaag aacaccggct agtccttttg atgctgaaaa
    12121 tcatggaggc ggtctgcaaa aagctggagc aaaaccagtc cgtgaaaacg gaagaccttg
    12181 acgccatcat cgatttcatc cggggttttg ccgacggttg ccaccacgcc aaggaagaaa
    12241 aactgctttt tcccgcgctg gaaaaaaccg gcatgcccca aaccgacgga cccattgccg
    12301 tgatgcttcg cgaacacgcc ctgggccgcg gttttgccag aagcatgagc gacgccatcg
    12361 gcctcatgaa aaacggcgac gcgacggccg ccaaaacttt tgtggaaaac gcccgcggct
    12421 atatccaact gttatggaat catatcgaca aggaagacca tgtactgttt atgatggccg
    12481 aacagcgcct gagtcccgcc gcgcaggatg aacttgtgga aggcttcgac cgcgtggagc
    12541 gcgaagagac gggcgaagga gttcacgaaa agtaccacgc cctgctgcac cgcatgcgcg
    12601 acgattatct gtagaaaaaa gcgttggaat gtctattcca agaaaaaagg ggtccgccat
    12661 ttattggcta accccttgat tttactggtg ggtcagggca gaattgaact gccgacactc
    12721 ggattttcag tccgatgctc taccgactga gctaccgacc cgaataacag gcaaaacctg
    12781 tttaaatgcc gaaaacgtct ataaaacttg attccctttg tcaaggcatt ttttgtccgt
    12841 ccactcccga cgcgccctgg acggaaggcg catcaggctt attttcttcc ggttgtgaaa
    12901 cgttgacggg gacgcagccg aatccggcgg gactttgcgg tcctgatgaa gaatcaggga
    12961 tcgaaattcc ggattcattt ccccgcggtt cgcggatgat cactaccgtg ccggcaatct
    13021 tgtcatgcca gccctgcttt ctccggtcaa aggcaaccca gaggtatccc aggcaaaaaa
    13081 ccaggctgga aaccaggtag ccgaccgatc tcaaaaaagc cgtgccgaaa gaaatcgatt
    13141 cgccggtgac ggacaccacc tgcaggccca gcagcctttt tcctggtgtg cgtccggtgg
    13201 agccgtgaaa ataggtaaag taggcaatat tgaccgcaaa cgtcagaagc cagatccagg
    13261 cggtcagcaa agccagtttg tccgggtccg tcatctgggc gacgattgcg ctgccgtctc
    13321 cggacattgc gccggcaatg taggccacgc ctgccacaac ccccagaaaa atgatcacga
    13381 cgctgacgat gaatccgtcg atcatgtatg caatgaaacg ccgccagaag cccgcgaatg
    13441 cgtatgtggt catttgccgc ctccttccgc gacggagctg aagatgcctt tctcgtactg
    13501 gatgatcgac agaattgccg ccgccgctgt ttccactttt aaaatctgcc ggccaaggct
    13561 cacggaaaca aagccgcttt ccctggcccg atccacttcc tcccgggcaa gcccgccttc
    13621 cggtccgaca ataataaaat aatgcgccgc gccggaaaac cggtcatcct gcaaaacgtt
    13681 ccgaatcgtt tgccggtcct cttcctccca gaaaatcagc ttccgcgcgc cggaatccgc
    13741 acgtttcagc atgtcggcaa aggacaggac cggctccacc acggtcacgt aggaattccg
    13801 cgtgcagcgc gccgcttcct gaacgatttt ttgccagcgg gcgactttcg cggacgattt
    13861 ttccccttcg attcttcgga tggaacgggc cgccgccacg gggatgatga cgtccgttcc
    13921 cagctccgcg gccgttttga cgatcccgtc catttttccc gctttgggaa tcccctgcgc
    13981 cagcgtcagg cggatttttc tcttcaccgg cggaaggagc ttgcccagac gaattgcaac
    14041 cccggacggg ctgaaatttt caatccgcgc ttcaaactcg tggccgtagc cgtcaaaaac
    14101 cagaacggcg tcgcccggct tcaggcgcag aaccgtcttc agataccgaa gctgatccgc
    14161 gccgagcacg cattgcgaag cgttttgcag cgtctcggga ctgtagattc ggggcaacgt
    14221 caacgtcaga cactttccag caaaaaagat tggcttgccg cctgaaagcg atcaggacgg
    14281 tccgccgttt tccttgcgca gcacgcagca gacccactct ttttctgtca gacggcggtg
    14341 ctgcaccagc ggcgcaacaa tgaaatgctc ctccattgcg ggcacgtcgt tttcaatgat
    14401 ccccgaaatg atcagataac cgccgggcag gagaagccgg acgagctgcg ggcgcagttt
    14461 gaggagcagt ttggccgtca ggttggcaat gatcagattg cgcggacggc tgatgcgcgc
    14521 tgcgtcctgg ttcaggattt gaacccggtc ttccacagcg ttgatgaccg tattctgggc
    14581 cgcaatctcc gtggccctgg gatcattgtc cacgcagatc acatcctgcg cgcccatctt
    14641 ggcggcggta atgcccagaa tgccggtgcc gcatccggca tcaagcactt tccattcgcc
    14701 agtcgagcgg tccttcagaa tgatatcctc tatcgcttcc atgcacatgc gcgtggacgc
    14761 gtgctgaccg gtcccaaacg ccatgcccgg atcgatctcc acgacaatgt cgcggctgtc
    14821 cggcgcgtat ctttcccagg tgggtttgac gacgatattc tggcaaactc ggatcggctt
    14881 gaaatatttt ttccactgct ctccccagtc gggatcggtg atggtttcgg aagaaagtac
    14941 gggcacggcg gcgtccggaa aaatttcttc cagacttttg agatattttt gaaccgcctt
    15001 catccgcttt tcggcgcgca cgtcggcggg aaagaaggcg ttgatgactt ccagtcccga
    15061 ggattcggga aaatcatcgg cgccgggcgg catcagcgat tctgaaaaaa cgccctgcgc
    15121 ccccgtttct tccagaaaat tgctcagggc gtcgatttgt tccgcggacg ccgaaatctg
    15181 aattttcaac catttttcgg gttctttttg cgtcatggcc cagccttcaa tgaggtagtg
    15241 tgaaaagcgg cttttatata gcctgttctt ccgaatatat caagcagccc tgtcatggcc
    15301 tgcggcctgc agaatttggc ttgacaaaaa cagtctcgga gcttattctg acgctggtaa
    15361 taccaccagc caggaaggcc gctgaggaga tatgatgcag gaaccccttg ttgaacccat
    15421 ccgcacagac agcttaaagg agatcttcgt gctccggttt gaagagctga ttctttccgg
    15481 aaaaatcaag atcggagaga agcttccgcc ggagcgggag cttgccctgc agctgggcgt
    15541 gagccgcccg gtggtgcacg aaggactggt cgaactggcg tcgcgcggac tggtcagcat
    15601 caaaccgcgc ttcggcgcca ccgtcaacga ttaccgcaag gaaggctcca tcgccctgat
    15661 ggcctcgctg attcagtacc agaaaggaaa tctggaaccg gagcttctgg aaagcctgct
    15721 gcagattcga atgatgctgg agccgccctt tgcgcggctg gcggcccgca accggacttc
    15781 ggagcaaatc ctggaactcc acggcatcct ggaaaaggaa gcggcagcca acggccggaa
    15841 tcctcaggcg ctcacggacc tcgattttga atttcacctg ctgattgcgc tggcttccgg
    15901 caaccgggtt tacccgcttc ttctgaattc gttccggtcg gtttatacga acctttccgg
    15961 ccagtttttc agaaacacga aagtaaccga cgccgtccac ggttatcacg aaaagatggt
    16021 caacgccgtg gaatccggca acgaaaccag ggcagcctcc gtgatgaagg ccattctgaa
    16081 ccacggggaa aagcatcttc gggaaatgat cgggcaggaa agcctgtccc tgtaaccttt
    16141 tttaccggga gggaaccatg aaaaccgcac tggcaaacga agttcatctg aaagagccca
    16201 aagcattatc cggccgcatt cagtggctgc gcgattatta tttccgcgga accgagcggc
    16261 cctggaacaa cgaatttacc tgctggacga ccggaactcc gtgggacgtg atttacaacg
    16321 agctgacgtt ttacatcgtg ccggaaacct acacgctgct caacacactg ggcgcatcct
    16381 atcttcaggc cgcgcggccc gtggcgctgc accgggattt ctggacatgg ccgctcgtgg
    16441 aacggcgggc ctggtttgtg aaggaagcca tcgtaaacta cgtgccgcag gaaattcttc
    16501 cgggggattt gctcgccggc gcgaggttca atattcaaac gtccatgtgc ttcacaaaag
    16561 aggaaagcag ggcctggcaa aagctggtca agggcaaaaa cggcgcaagg gcgaaaatga
    16621 aatggttcca tgaccacgga tacggaaacg ccggggccac cagcggccat ctgatccccg
    16681 gccatgagcg gctgctgcaa attggctgga aaggcgtcca cgccgaactg atgtcccggt
    16741 ataacgccct cagcagcgct gagcgagccg catccaaggg cgcacagctc cgcgccatga
    16801 tgaccgcgtc caccatggcg cgcgatctgg ccgggagata ccggtccctg tgtgaggatc
    16861 tggcgcaaaa ggagacggac gccgtccggg cggccgagct tcggaccatg gccgcgaatc
    16921 tggcccgcgt gccgtgggag cccccgacga atttctggga agccgtgcag gcgctctgga
    16981 tcaatcacat gctggtcatg acggacgaga actacccggg cgcgggcgtt tccttcggcc
    17041 gggcggacca gtatctttac ccgtactacg aaaaatctct ccgcgacggc atgcagaggg
    17101 attggaccaa ggaaatcctg aaatgcttct gggtccacgc caacacggca tacgacgcca
    17161 tgatccgcaa cggcaatcag ggcattaccg ccggcttcgg ccagttgatc acgctctccg
    17221 gcctgggggc cgacggacgc gacatgacca atgatctgac ttacgccatc ctggaagtga
    17281 tcgatgaaat gtcgcccatc ctggagccca aaccgaatgt gcggctgcat cgcaacagtc
    17341 ccgaagagct ctacgacaaa atcgtggaca tgatcgaatc cagccagggc gcgcctttcc
    17401 tgttgaattt cgacgagcgc tccatggccg gcatgatgct ggaggcccgg aaggcggggc
    17461 ttgcgcatct gatccacgag ggcaacgtcc acgaatacgc gcccgtcgga tgtctggaaa
    17521 acaccatggt cggcaatgac cgctccggaa ccgtggacaa caacctcaat ctgctcaagg
    17581 ccgtcgagct ggcgctcacg ggcggaaaag acctgctgcc cttttatgat cccatgaccg
    17641 gaaaaaccga agccgttcgg caggacggcc cccgaacgcc ggacgccgcg tccattcaaa
    17701 cgtgggaaga attctgggaa gcctatgcgg cccagacgcg ctacatcatt caaaagtgcg
    17761 tggacctgta cgaaacatcc gaatccatcc gcgcccgatt cttcccgacg ccctatcttt
    17821 cctgcatggt cgagggctgc gccgagcagg gcctggacgt tacgcaaggc ggcgcccaaa
    17881 tcagcttcac cacgctggaa gccgtgacct acgcgacaac cgtggattcg ctcctcgccg
    17941 tcaagtatct ggtctttgac aaaaaagtct gcacgctgcc gcagttggcg gacgcgcttc
    18001 gggccaactg gaaggggcat gaagtgcttc aggccatggc caaaaaccgc gcccccaagt
    18061 acggccggga cgatgatgag gcggacgaac tggcgctgcg cgtcatgcaa ctctggtgcg
    18121 cagagacgtg gcggcacagg acccagtcca ccgggcgccg gtatcgaccg ggaatgctga
    18181 gctggaacta ctgggcgggc gacggctaca tcatggcggc aagcgccgac gggcgcacca
    18241 agggtcagtt tctctccaat gccatctgtc cgtccaacgg ggcggacatc aaaggaccga
    18301 cttccaacgc caactccgtc gggaaagtac tgggcggcaa agccgaagac ggccggggcg
    18361 actggctgga ctatctgaac gtccttccga acggcgcgag ccacaccatt acgttcaatc
    18421 cgtcgattct gcgggatccg gtgcacaagg ataaattcaa agcgtttctg aaaggctaca
    18481 cccagaatgg cggaaccgcg ctgcagatca acatgctgga ttccggcatg ctgaaagacg
    18541 cgcaggatca tcccgaaaac taccggcatc ttctggtgcg gatcaccggc tacaatgcct
    18601 atttcacaac cgtgggaagg gaactgcaaa atgaagtcat tgaacgggtc agtcaccagc
    18661 agttttaagg cggagaaccc atgacgtcgt ccaaaggaaa aaatgggctg gttctgcaga
    18721 ttctccgcat gtccacggag gacgggcccg gcattcgcac gagcgttttc ttcaagggtt
    18781 gtcctctcgg ctgcctgtgg tgccacaatc ccgaaagcat cagccccaag cctcaggttc
    18841 agtggcttca cacgagctgc atcggctgcg gcaactgcgt ggaaatctgc ccggaaaaag
    18901 cgctggagaa gacgaccgac ggcatcatga tcaaccgccg gctctgcacc ggctgcggtt
    18961 tgtgcgcgga agaatgcccc acgaccgcca tggagcttct gggcaaaaaa tggagcgttt
    19021 ccgatctcgt ccatgaactg gtcaaggacc gcgtttactt cgaacagtcc ggcggcggcg
    19081 taaccctgac cggcggagag gcggccctgc agcacgaatt ctgtctgccc cttttgaaag
    19141 agctgagagc cgtcgggatt tctacggccc tggacacctg cgggcaggcg ccccaaaaaa
    19201 cgctggcttc ccttttgccg tacatcgatg tcctcctgta tgacctcaag gagatcgatc
    19261 cggaaaagca caaaggattc accggagcgg gcaacgcgaa aattctggaa aacgccgtct
    19321 tcgcggcgcg cttcaaaaaa gaccatccgt atcccaggac gctgtggatt agaacgcccg
    19381 tcattccgaa ttcgacggac acgcccgaaa acatcgcggg catcggccgg ttcattcacg
    19441 aaaatctgga aggagcggtt gaccgctggg agctttgctc cttcaacaat ttatgcaggg
    19501 acaagtacaa acggctgggc ctgaactggc cttttgccga agcggaactc atgaaaaaaa
    19561 ccgtcatgga aaatttggcc ggtgtcgcga aaagcgcggc tccgaagacg aacgtctgct
    19621 ggagcggatc gacgacgctg gaaaaaagag cggattcaca agaaccgccg gagaaacaga
    19681 acaaacgcaa accggtttgc gccggctgat ttcaagagaa aggaaaaagt ccatgaccat
    19741 caaaaaccgt ttttcggaat cggacatgaa ggcctttgcg ccgtcggaga aagtcggcat
    19801 catcgcgacc gtgacgccgg aagggctgcc gcacctgtcg cttctgacct cggtcatggc
    19861 ctcaacgccc acgcagttga cgatcggcga gtttagcacg ggcgtgagca aagcctacct
    19921 gcagcagacc cggaaaaccg gttttgccat tgtgacgctg gacaagaaac tctggcgggg
    19981 gaaagcgctg tggacacact tgaagaaaga aggcccggaa tacgaagtct ataatcagca
    20041 gccgatgttc cgatacaata cgtatttcgg aatcaacacg gtccactatc tggagctgaa
    20101 ggaaaccacg gaagggtcgc cgctgcctct ggttgccgtc gcggcgtcgg cgattctgac
    20161 cagactcgcg aaaggcgccg ccgcgcaaag cgcgaaagtg aaggcgcttt cgccgttcgg
    20221 gaaaaatctg tttgaccgcc tggattccct caaattttta tcgttcattg acgacgacgg
    20281 atttccgacc gtcatccccg ttattcaatg ccaggcctgc gacgaaaacc gtctggcgtt
    20341 ttatccgggc gtatttgccg aagagctgaa tcggctggag gcgggaaaga caatggcgat
    20401 tttcgggatg accatggaga tggaggatgt tctggtccgg ggcgtctttc agggctacgc
    20461 ccggcgccgg ggcatcaggc tgggaacgct ggatatagac tgggtctata attccatgcc
    20521 ccccaaccac gggcagattc acccgccgct tcctctgaaa gcggtgacgg atttctgact
    20581 ttttcctgct ttttcctgac ctgctaccga tggaagcgca ccggaaggac ccggacgtcg
    20641 ctgtccaagc cctgttgcga acagacgctc tggccggtga tgaacgacag gttgaaggcg
    20701 tagtaaccgg ccatttcata cttcggaagc atggaaatgc aggtatccga agtccagggg
    20761 cagcagcccg tcaggctaat gagggatgtg gccaggtgat acccgtgagc gtcacgactt
    20821 gagccgctgt acaatcccgc caattcattt ttcgtcggca tccgccagtc gtcgtatccg
    20881 cccgtgctga agttttcaca gaatgtcttg gcggcaatcc attggatatc gactccgttg
    20941 tccttatccg cccacatcag attggtcgtg gtatccagga cggtcccgtc gtcataggcg
    21001 acaaaccggc catccctgcc ggtttcattg gccgcaaacg cggatccggc gcagaccata
    21061 atccccagca aaacccatac aaccatcatg attccacgtg tttttctcat catcaccctc
    21121 gctgtacggc tacttttgaa aaaccccctc catcacgatc tcatccaact tttttcgcgg
    21181 cgaagggaat tcccgttcct ggagatattc gggaaggtct tctttgttcc cggggcggcc
    21241 cacggcaatc atcgcctcca cggcgtagtc atccggcact cgcagcgctt ctttggcttt
    21301 gtcatagtcg aatccctgca tgcaatgcac caccagattt ttcagcgacc cctgcagggc
    21361 cagactgacc cacgcggccc ccgcgtcata cgaatgcgtt cgcgcggggc tgccggaatc
    21421 aaacgtggtt ttggaaatca cggcgatcag aaccgcggcg tttttcgccc agattcggtt
    21481 gccttccgcc agaagctcaa agaaaacctc ccagcgcggc gtatcgcggc gggcataaat
    21541 aaaccgccag ggctgattat tattggcgga aggcgcccag cgcgccgcct caaaaaggtt
    21601 cagaagcgcc gcctcatcaa gatccgcgcc ggacatggcc cggggagacc agcgatgaag
    21661 aaatatttca tgaacaccat tttcctgctg tcgaaagtcc ttgatattca tcgattcctc
    21721 cccattgccc aactgccgtg gcgctgtttt ttaacaaaga ttaaccggtt ttcttcagaa
    21781 tgcaagcctt tttaaaaata gtggagtctg agcttgactt tccgggttta aaaatatata
    21841 aggcgcaact tgctgaaaag caggccctct aaaacagcgt ccccatcgtc tagaggccta
    21901 ggacacgggc ctttcacgcc tgtaaccgcg gttcgactcc gcgtggggac gccaatttgg
    21961 ggtcaaatct ttattcttga catttgacca actcattttg aatttttatc aattctcccc
    22021 caagttccgg gttgttttct attttgattt gcagccgttt tcgtgcctgg ctcaccgcac
    22081 tgtaatctac gccgcccagg agcgatccga tttccggctg cgtgagattg caaatccggt
    22141 ataagatttc catcagcatc gcccgctggg tcgattgttt gccttttctg gtcaaatcct
    22201 ctctgctgat tttcaccagc cttgcgtatc gatcaatcaa atgatcgggt ttaatctgtt
    22261 ttcccagctt tctaagcgca ggctgctccc gggaagaacg ctgatgcccc ttgcttttgc
    22321 tgaatgtctt ctgaatttgt tcgataaaat cctcgcctcc aacaatacca gtaccctttc
    22381 ccttctccag cggactgggc aggtctcttt ccatgccttc cacgacaaat ttttgatatt
    22441 cattcatggc attgtgattc tcgccgccaa aataatccag aatgatttta tattgaacaa
    22501 agcttttcct gttttgcggt tgaagatatc ccggaaaact ggtcgaatca ttgatcatca
    22561 aatcccgaaa tctttgcccc acgggctttt gagacttaat tctcagcgga ttcaaatgga
    22621 tgtagcggga aacttccata agataattgt tttcatctat gagaaaagct ttgtacctcc
    22681 cctggtagag atgcccggtt cgtttatgtt tccggttaaa aaaaccggta tagctgatgt
    22741 tgaaatgacg catgaattcg gaaaggttgc ctttcggtgt gcaaacaagc aggtgaaaat
    22801 ggttgggcat cattgcatag gccagtagtt gaacctcaaa gatagttaag gatcgactca
    22861 gcaaacgaaa gaattctttc cggtcatcgg catgacgaaa aatttccctg cgttcattcc
    22921 cgcggcaggt gacatgataa aaagcatccg gatattggat tcttagggga cgactcatgg
    22981 tgttttttaa tagagagagc ttgaaatgtc aagaataaag atttgacccc accctgcccc
    23041 aagaataaag atttgacccc accctgcccc ccaccctgac ccttatcgca ttttctgtcc
    23101 cgccgcatcc gcggcgatca tggcgtggag cacacgctcg ggagtgatgt cgcccgcttc
    23161 gtggtgaaca gccgcgccgg gcgcacaggt cttttccgcc gcttttctca gtccgattcg
    23221 gtcccgttta tccaatccga tatctgcaag cgtaacgggc aatcccattt ccctgcaaaa
    23281 tccatagacc gttcgcatca cttccggtga tgcgcccgtc agatgaaggc cggtcaatac
    23341 gccgaaggcc accttttcgc cgtgataata ggcattggtt tcctcaaggg cagtcaagcc
    23401 gttgtgaatg gcatgagcgg cggcgagccc ggaactttca aacccgattc cgctcagaag
    23461 aatattcgct tccacgatgc gctccagggc gggagtcggc acctgatgct cgcaggcgag
    23521 tttcgccgcc gctccacagg ccaggagcgt atcgtagcat agcctggcga gatgcaggcc
    23581 cgccattgtg ctcagtccgc cgcattcgtt tttcgattgc gtacggcggc aggattcagc
    23641 ctcaaaccag gtagccagcg cgtcgcccat gcccgccacc agaaaacgaa cgggggccgc
    23701 ggcaataatg ccggtatcta ccaccactgc ggcgggattt gatttctggt agtattcccg
    23761 ctcgtggacg ccgttctcgg aatagaccac cgcacagccg ctgcagggcg cgtcggtgga
    23821 ggcaatggtg ggaacgatga tcaccgggat attcgcccgg tccgcggcaa ttttcgccgt
    23881 atcgatcgtt ttgccgcccc ccatgccgat cataacgccg atgcggttct tttcaatgac
    23941 cttggccaga cggactaatt ccttttcgca gcactccctg ccgaatgttt cgatgaacgc
    24001 ctggtttgtt tcttcatgaa tgccgcagac gggaacaatg gttttggctg ccgtggggga
    24061 tgcgaggaca agcgccctgg cgccgaagag tgagatcagt gccggcaatt ctttcagcgc
    24121 tcccgcgccc tgaatgtatt ttccggggaa taccgctttt ttaaacatgc ctctcctcca
    24181 tttctcccat ttcgtatgta aaaagataaa tatcacaaaa tggccgcaat cccatctgtt
    24241 tttatacttc aaaagccatt tgcgatgaat ggcgcaagat tttcttgact tacaaagcct
    24301 cgttttttat actccgcaaa aattatcagt tcacagtgac aaagacaaag attgcaataa
    24361 gacagcctcc tgatcataca ttcgtttatt tgcggatggg aagggcttcg catgatcatg
    24421 catggcgcag ataacgcccg tttttggacc gatcagctgt tcaacgcaat gattacggcg
    24481 gaccgccggg aggcgtccgg cgtaatggac cggatgatgg ccgccggtgt aaattcccaa
    24541 tcgatcattg atgacgttct cgatccgacg cttatccggc tgggcgacct ttgggagcag
    24601 gacgccgtat cgctcaccca gaccttcgta gcctccaaga tcgccgaaga tgtgctgctt
    24661 cgctgtgtgt cggcttcagg aaaaacggca aagcggggcg cgccggttgt catcggcaat
    24721 atcgaggatg atttccacag cctgggacgg cgcatcgtgg gatctttttt gcgcgcgggc
    24781 ggctgggagg ttcacgacct gggcaatgac gtgacggccg aacagtttgt ggacaaggca
    24841 atcgaggttg gcgcgtccgt tgtaggggct tccgcgatga tgcacaccac ggccctgaat
    24901 atccgcaaac tgcgagatct gatcgacgcc cgcgggctga atcatcagct gaagctggcg
    24961 gtgggcggcg ccgtcttcaa ctggcggccg gatcttgtcg cggaagtggg aggcgacggc
    25021 acggcccgca acgcaaccgg ggttgatgct ctgtttcaaa ggctccgcga cgcagcgaaa
    25081 ggagggtgag caccatcaat tcgtgcgaac gcgtgatgaa tacacttcag ggccttccga
    25141 cggatcgcgt gccggtcttt gccgtgctgg gcgcctacgg cggaaagctt gccgaaaccg
    25201 gcctgaaaac cctttacagc gatgcagatg cctacgtctc gggacaacag gccgtccaga
    25261 atgccctggg tttcgatatg attctggcca ccttcgatta cagcgccatc gcagaggcgt
    25321 tcggcggcga ggtggcctgg tttgacaatc aaacgcccaa catgaaacgc cccgcggtca
    25381 gtgaagcctc cgcagttgcc ggcctgaagc tgcccgatcc gcggcgagac gcaaggctgc
    25441 cggtcattct gtcggcaacg cggcaactgg tctccctgta ccgggaaagt gtgccggtgt
    25501 ttgcggcggt gcctggtccg ggggcgctgc cggcgctggt gctcggactg gaaacgtgga
    25561 tggaaacggt tttattcgac gaggataacg cccgccgcat tctggagcgg accggccggt
    25621 ttttcctgga ctgggtccat gcgctttgcg aagcgggcgt gaccggtctt gtggtgacgg
    25681 aatccgtcgc ctccgtggaa atttcgacgc gcgacctgtt tgcgaaagtt ttcctgcccc
    25741 acctgcacga tctgttcggc caaatcaacg cgcccctcgt gtttcaccac ggcggcggcc
    25801 gaattctgca tattctcgac ttgctgcccg gtttgccggg attggcgggg gtggtcgtcg
    25861 gctccaagga tgatctgggc gccgcaaggt cggccctcgg aaccgaattg accctgatcg
    25921 gcaatctgga taatctgagc ctgccggccg ctacattctg tcgcactccg ccgcggatgt
    25981 gccgctgagt acaccgc
//
LOCUS       DAOPAF010000021        24836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2167_length_24836_cov_49.607603, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000021 DAOPAF010000000
VERSION     DAOPAF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24836
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2167_length_24836_cov_49.607603"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PLQ77_03760"
     CDS             <1..149
                     /locus_tag="PLQ77_03760"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPL09825.1"
                     /translation="AGVRVLLDSTDGSELWSTTGVSENIIEASWQALIDSIEYKLSKD
                     RKKR"
     gene            205..843
                     /locus_tag="PLQ77_03765"
     CDS             205..843
                     /locus_tag="PLQ77_03765"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPL09826.1"
                     /translation="MEAQRKARARTVLSINSQNPQMRLIRKATDVLRDGGIIIYPTDT
                     VYGLGCDLSNKRGIEKIYEIKRRNKKRPLSFVCSDLKHISQYALVTDYAYKTMKRFLP
                     GPYTFILEASRLVPRIILPKRPTTGIRVPDNQICLALIRELGQPIISTSVQTLDGEDL
                     GDPVIINEHFGRVVDLIIDGGIIVPEPSSVVSLVDDRIEVLRTGKGDVSAFI"
     gene            915..2786
                     /locus_tag="PLQ77_03770"
     CDS             915..2786
                     /locus_tag="PLQ77_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPL09827.1"
                     /translation="MKHVMLVNAVHKEQMRMATVDEKGKLIDFNIQMAVREPITGNIY
                     KGKVLKVERGLQAAFVDYGGVKDGFLPLRDVSHEYLTEPENGSSGGRAVLKTGQEIIV
                     QVVREIIGNKGALLTSYISLPGRYLVLLPNKLCGGISRKIESEEDRRKIKTLMDQIKV
                     PEGMGFIVRTAGINRTKQEIQRDYQFLMRLWKEITKKAEDASEPSLLYQETDFGVRSL
                     RDYLTQEMDEIWVDEVETFRKMRAYLKAVAPRHLRILKQYKDKTPIFERNSLEEQIRV
                     IYQERVELKSGGYIIINPTEAMITIDVNSGRGSNKRNIEETAFRTNVDACEEIARQLR
                     LRDLGGLIVIDFIDMIDKKHIAEVEKAFKKALSMDRARIQLSRISKFGLMELSRQKKQ
                     STIQEISYTTCPYCAGSGLRPSLEYASLGAFRKIESEAVKGTYSELRVSLPHEIALYI
                     LNNKRTELLKLESTFGVCLIIEGKPDMAWDNFEIQQSMKEQALEVSAIQGVPLLNLAE
                     KTEPDAEGGEKPEAEAGEAEEPPRKKGRRRSRHKKRKSPEALKGSVATEDALPDAQPS
                     PGGMAPPVSSSQFSRFPVKPLKIVGALPEEFFPVDLSGDLFTPEMDQEDTGPEQEDN"
     gene            2851..3825
                     /locus_tag="PLQ77_03775"
     CDS             2851..3825
                     /locus_tag="PLQ77_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL09828.1"
                     /translation="MADEIRKGLFRISVPLPNSPLKDLNSYVIRGEDRNLIIDTGFNR
                     TVCYEAMQAGLAELGIDLNQTDFMLTHMHADHTGLVTRLATKTSRIFFGRIDARVFDG
                     DHGWQSLIDFAALNGFPVDELQKALASHPGFKYSPQQKPVFTLLDDGDVIECGGYRLR
                     CVATPGHTQGHICLYEEDQRIFFSGDHVLFDITPHIESWAYETNSLSDYLASLDRVYA
                     LPVELVLPGHRNFQGDLKDRIRELKHHHRERADEVLEVLGGRAMNAYEIAAGMTWDID
                     CETWEDFPIAQKWFATGEAIAHLRFLEGEGRIHRHTDQAVVAFSAVRP"
     gene            complement(3923..4381)
                     /locus_tag="PLQ77_03780"
     CDS             complement(3923..4381)
                     /locus_tag="PLQ77_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPL09829.1"
                     /translation="MSIESVLKELQPHLGKEVFVSGWKQVTQAQINQFADSTGDHQWI
                     HVDAEKAAAGPFGRTIAHGFLTLSHIPFFSYQAPVKIEGAKMSINYGLDKVRFLNPVP
                     SGSNIRDRIVLSGVEEKPGNRLLIRQTHTIEMEGQDKPACVAEALAMVFF"
     gene            complement(4492..5529)
                     /locus_tag="PLQ77_03785"
     CDS             complement(4492..5529)
                     /locus_tag="PLQ77_03785"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPL09830.1"
                     /translation="MKTRITEMVGIKYPIVLSGMSWISVPKMVAAVSNAGGLGILATG
                     PLNAAQTREAIREIRKLTDKPFGANASLMFPGAVENAKVLLQEKVPVINFALGKGDWI
                     VKEAHKYGGKVFATVVNLKHAKRAQDFGTDAVIATGQEAAAHGEDVTSLVLIPHLAEN
                     LKIPVIAAGGFADGRGIAAALALGAEGVAMGTRLMTTRESPLHENYKRMTQEKDVYDT
                     LFSKRFDGILCRVMKTDAAKKAVKTGLNWPAAFFNSQAIAKQMGIPYFKLFIGVLLSG
                     YKNARQLAYMANAFKAIRVATEDGDSKTGVLPVGQSQGLIHDVPTIAELFERIMKEAK
                     AAQAKVNAALE"
     gene            complement(5573..6565)
                     /locus_tag="PLQ77_03790"
     CDS             complement(5573..6565)
                     /locus_tag="PLQ77_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPL09831.1"
                     /translation="MFDTQITRMFGVKYPVICGAMMWLCKPKMCAAVSNAGGIGNLTA
                     GNYETADEFRAAIRETRRLTDKPFFVAITLLPSVRITPEHHKMYVKVCAEEKVAGIEF
                     SGTPLDKVAGMEAVEMLKKAGVKMFHKVGALRHALHAEKIGFDGIYAAGIEEGGHPLN
                     DDVTSMVLTPKIAASVKIPVVTVGGIADGRTMAAALALGAEGVMMASRFIATKECEVH
                     DNIKQELIRRQEFDTVIYGKSIGLQGRGLKSKVIEEVLAIEARNGGFEELIPLLSGQK
                     VKQAWETGDVDYAPLMVGQSIGLINDIPTCAELLERMAAQAKEITARTAKLCGN"
     gene            complement(6576..7304)
                     /locus_tag="PLQ77_03795"
     CDS             complement(6576..7304)
                     /locus_tag="PLQ77_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPL09832.1"
                     /translation="MKLKIGEIAKRSSVRPSTIRYYVGEGLLPKPEKANEKMAYYDEA
                     CIERLRVIQELKERRYFPLYLIKNILRRMDEGLNLTEAETIENAVFRSGQQAENGLIG
                     QAAFLDATGLTEKELAQARRLGILVPFTFERDKPLYDPDDVRAGRDGIKKMADLGMKL
                     QELVFWVELGRQIVEKEMALRRKMVAGKSTRENIAITAELTQVGNFYRDYILRRLFQK
                     QVEQNIQSSLKAAKKRKTGNNGKK"
     gene            complement(7469..8263)
                     /gene="trpA"
                     /locus_tag="PLQ77_03800"
     CDS             complement(7469..8263)
                     /gene="trpA"
                     /locus_tag="PLQ77_03800"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPL09833.1"
                     /translation="MGRIGKKFEELRAKKEKALIVYATAGDPSIAATRELALSLEKSG
                     ADILEIGVPFSDPTADGPVIQAASQRAIKAGTTLRGVLDLVADIRRASRIPIVLFGYF
                     NPIFAYGAQKFSKAAKDAGVDGILVVDLPPEEAGELRVFTNAAGIDFISLVAPTTGDD
                     RLKAILKAASGFLYCISITGVTGTAAPKTEDIAREISKIRKLTKLPVAVGFGITSAGQ
                     ARAIARIADGVVIGSSVVKLIGENQNGGDGIRAAAAHVKEIKAALL"
     gene            complement(8422..9603)
                     /gene="trpB"
                     /locus_tag="PLQ77_03805"
     CDS             complement(8422..9603)
                     /gene="trpB"
                     /locus_tag="PLQ77_03805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPL09834.1"
                     /translation="MKMLPDQKGHFDAFGGRYAAETLMPALLELEEAYAMAQKDKSFE
                     KEFDRYRRDYAGRPTPLYFACRMTQALGGARIYLKREDLNHTGSHKINNTLGQALLAR
                     RMGKKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKILGAEVV
                     PVQSGTATLKDAMNEAMRYWVGSVRDTFYIIGTTAGPHPYPMMVRNFQSVIGREVKKQ
                     TARAEGRNPDVLVACVGGGSNAMGLFYPFRNEKKVKMIGVEAAGRGMNTLEHSASING
                     GKIGVLHGNKTYLLQDENGQVRDAYSIAAGLDYPGVGPEHAYFAQSGRATYVTVTDEE
                     AIGAFLYLSRQEGIIPALESSHAIAHAMKIAPKMKKRRILVICLSGRGDKDARTIMET
                     L"
     gene            complement(9600..10253)
                     /locus_tag="PLQ77_03810"
     CDS             complement(9600..10253)
                     /locus_tag="PLQ77_03810"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPL09835.1"
                     /translation="MQVKICGITNLEDALTAAENGADAVGFIFHPPSPRFIEPQKAAQ
                     ITKALPDRLVKVGVFVNEKESFVRHTFEHCRLDMIQLHGDESPGYCRQFPKNRILKAL
                     VLQTEEDVRKSEAFDVAAILVDARHNGLYGGTGRTADWDLARKIAGPLILSGGLNEFN
                     VPAALEVVRPAALDINSGAEEKPGKKDSAKIARMMRIIHSASAGETPLNIFIGRDRT"
     gene            complement(10244..11029)
                     /gene="trpC"
                     /locus_tag="PLQ77_03815"
     CDS             complement(10244..11029)
                     /gene="trpC"
                     /locus_tag="PLQ77_03815"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPL09836.1"
                     /translation="MILDKIVETKKEEVARLKKEATTERLCEAIAALPPCRDFRAALG
                     GADCAVIAEVKCASPSRGRMLQHFDPAAIAKTYEKNGAAAVSVLTDQTYFLGHTSDLT
                     SVRREVRLPVLRKDFIIDPLQVYETRAVGADAILLIVRALGPRLFEFIALSEALGLCP
                     LVEVHTEEELQAALAAKAEMIGINNRNLDTFATDIQTSCRLRKQIPGDRTVVAESALK
                     NRADIEHLMNAGISSFLIGEALMTAPDIGRALRSLLGRESSCR"
     gene            complement(11160..12170)
                     /gene="trpD"
                     /locus_tag="PLQ77_03820"
     CDS             complement(11160..12170)
                     /gene="trpD"
                     /locus_tag="PLQ77_03820"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPL09837.1"
                     /translation="MIREALDKVVQNIDLPEGDMMAVMEEVMEGKATPAQIGSFITAL
                     RMKGETVEEVTGAARIMRRKAARVNACATTVVDTCGTGGDKLGTFNISTTTAFVAAAA
                     GIVVAKHGNRAVSSGCGSADVLEALGVNISIDPEIVEECLQQIGIGFLFAPRLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLAEMFAGVLKNMGTKRAFVV
                     HGVDGLDEVTVTGETRVAELKDGIIRTYNLSPKEFVGTTHPLEAIRGGDPEVNARILT
                     DVLSGKPGACRDVVLLNAALAIVAGEKAGNIQEGVKIAADCIDSGRAAKILRALIEMT
                     NG"
     gene            complement(12167..12754)
                     /locus_tag="PLQ77_03825"
     CDS             complement(12167..12754)
                     /locus_tag="PLQ77_03825"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPL09838.1"
                     /translation="MILMIDHYDSFTFNIVQYLRQMGEDVRVRRNDRITLEEIRRERP
                     EAIFLSPGPCTPRETGVTIDVIQKFYSSVPLMGICLGHQCIGAAFGGEVVRAERIMHG
                     KVSPVEHDGKTIFAGLPQPFAAGRYHSLLVRPESLPPCLEISARTPEGEIMGLRHKNY
                     PVEGIQFHPESVLTPQGKRILRNFLKQIGRKEHGS"
     gene            complement(12751..14253)
                     /gene="trpE"
                     /locus_tag="PLQ77_03830"
     CDS             complement(12751..14253)
                     /gene="trpE"
                     /locus_tag="PLQ77_03830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPL09839.1"
                     /translation="MYSPKFADFEVCAKRGNLIPVYREILADLETPVSVLKKIRHKRH
                     AYLLESVEGGEKWGRYSFIGTNAEIVFRIRGPKVILEEKGKIFETEHRGDPLGRMREL
                     LGRYRPVVPPGLPRFFGGAVGYLGYDTVRYFEKLPDVPPDDLNLDESVFVISDSLIIF
                     DNVRHTIKVVACAFAEDEDSRAEAYAAACRKIDDLIALLQTPAEYPAPAQKASVVVFE
                     SNMTPDRYKAIVEKAKDYIAAGDAIQVVLSQRFSTDCTADPVDLYRALRCVNPSPYLF
                     FLKLDDLTLIGSSPEVMVRLEQNEVELRPIAGTRPRGKNEQEDRALADELLSDEKERA
                     EHVMLVDLGRNDLGRIAQTGSVQVNQYMVVEKYSHVMHLVSNVRAELDCGRDAFDVLT
                     ATFPAGTLSGAPKVRAMQIIDELEPVRRGVYGGAVGYIGFSGNMDLCITIRTMVIRDG
                     RIFIQAGAGIVHDSRPEAEHMETLHKARGMQKAVELASSGFVLENGRSAL"
     gene            complement(14552..15310)
                     /locus_tag="PLQ77_03835"
     CDS             complement(14552..15310)
                     /locus_tag="PLQ77_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09840.1"
                     /translation="MAHREAMTLQNEIISLLPDRENYVVGFADMGDLMAGYYPYRYAV
                     VVGKRLDDAIVDEIEDGPTMPYYELYNSTNDELNGITAKISRFLTQNGISCLQIKATV
                     RAEDIPPDFGKTLRMNFSHKMAATHAGLGWIGKTDLLVSKAFGPRIRLATVLTHQPIE
                     PCGTPITESRCGACDICVRLCPGEAANGKLWNSTLDRDEFYNAFRCMEACRRLSQERL
                     NRNVHLCGICVSVCPLGKPSKRRNKKKIPQKTRA"
     gene            15527..16843
                     /gene="rmuC"
                     /locus_tag="PLQ77_03840"
     CDS             15527..16843
                     /gene="rmuC"
                     /locus_tag="PLQ77_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPL09841.1"
                     /translation="MDIIALVILISLLVIIVLLLWQLRRSSVDWTPLVGKLDALRDAQ
                     ERTDRSLRDEIARSRSEAQSNAQQERAELGASLSSFGDSVQARMTDIAKIQSDQMESF
                     ARQLSLLAAGNEKKMEDIRLMVDEKLRQIQEDNARQLDRMRETVDEKLQSTLEKRLGE
                     SFKQVSERLEQVHQGLGDMRTLAAGVGDLKKVLTNVKSRGTWGEVQLGALLEEILSPD
                     QYLKNVRIQESGGDFVEFAIRLPGQGDSPTESVLIPVDAKFPVEDYSRLMEAQERADA
                     PAAEDAVRQLEASIKKAARDISQKYLAPPRTTDFGIMFLPSEGLYAEVIRRTELVQHL
                     QREYRIVISGPSTFAAFLNSLQMGFRTLAIQKRSGEVWKVLGEVKSAFGRFGDSLDAV
                     RKRLDQASSSVDDAQKKTRTLAGKLKAVESLSDASGEGTVADTEAD"
     gene            16960..18090
                     /locus_tag="PLQ77_03845"
     CDS             16960..18090
                     /locus_tag="PLQ77_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09842.1"
                     /translation="MLKNKSNVDSSAASDKTQLYLMERVKELTCLYDMARIAADTSLN
                     IENMLMGIVGILPEAWQYPKDAFAKIVFNNRIFSMPNYPKFVQRQRSALVINGRRRGY
                     VEVAYPKKRGAQNLNLFLQEEQKLLDTVAREVSAMIRRRETEQEKLKLEDQLRHADRL
                     ATIGQLSASVAHELNEPLGHILGFAELAKKCPGLPVQAMADIEKILATSLHAREVVRQ
                     ILIFSRQASQQKTKVNLNNLIKQGLTFLESRCVSEGIKLQCRLGLDVPEIDAAPAQMN
                     QVFVSLMVNAIQAMPDGGTISIRTRRAGEKVSLNIEDSGVGMNEEILQKVFIPFFTTK
                     EADVGTGLGLPVVHDIIAAHKGSIKIESKVGMGTKCKIQLPV"
     gene            18066..19478
                     /locus_tag="PLQ77_03850"
     CDS             18066..19478
                     /locus_tag="PLQ77_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL09843.1"
                     /translation="MQNTTAGLKKPGALKQKEKKQPSDKKDDILIVDDAPATLELLER
                     NLSSVGYGIWMASNVEEALRVIDSSHIDLVITDFKMPGLSGVHLARHIRENYNDMAVM
                     IITAYPSVKGAVEAVKTGAEEYLAKPFTGEELLSAVKRVLRNLHIRRAAHASNKPRIH
                     IPHGLIGESGAMSKISNFINKAARTSATVLISGESGTGKELVARAIHYKSERASAPFV
                     PVNCGAIPEELLESELFGHIKGAFTGANETRAGFFQTADGGTIFLDEISETSLSMQVK
                     LLRVLQDKEVCMVGATRTRKVDVRILASTNKNIPALIKKGQFREDLYYRINVITVELP
                     PLRERQDDIFLLTRHFAAKFAEELGRTAPRFSDGALQVLRSYHWPGNVRELENIIQRL
                     VVMTDEDVIDVPDLPSLMRFSAHQPAAPDRTLAAVEAEYIRSVLLSVSNNKTLAAKIL
                     DIDRKTLREKLGKAKPSASR"
     gene            19920..20915
                     /locus_tag="PLQ77_03855"
     CDS             19920..20915
                     /locus_tag="PLQ77_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPL09844.1"
                     /translation="MGLCATCDDAKTCLYSKDEAGVWHCEEYRCDGCAKESGSPPSLE
                     KRGMDAKDILETIRKHDKNKGALIAILEEIQAAYTYLPEEALRLVSKETGRSLVDIYG
                     VATFYKAFSLKPRGKHCVSVCLGTACHVRGAQGIVDEFKRELDIAPGETTADNEITLE
                     TVNCLGACALGPTVVVDGHYFPHVSKSKVKNIIARAMAGLEKIDAETDNRVFPLQVSC
                     SACNKSLLDPEYPMDGYPSINLRVSCNGTRGRLRLSSLYGSGASVLEHPIPSNSLSRF
                     YCPHCAGELPSFSTCTECGSPMAAMRVCEGCAWEVCTRQGCEGHLLNLNQMNIQK"
     gene            20933..24181
                     /locus_tag="PLQ77_03860"
     CDS             20933..24181
                     /locus_tag="PLQ77_03860"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPL09845.1"
                     /translation="MKRFQSVAQFKTYRKTFLATSDQSKPCIVICGGTGGQASGSNDL
                     IRIIKRQILEKDLHDKLDLRITGCLGFCEMDPFIIVEPGYNLYPKLKMADVPRIIDAA
                     VRGKVVEELLYREPGSTVKRYSEKEIPFFQGQTRIVLGQNQRLDPIRIHDYLKIGGYA
                     ALEKALQKKDRAWLVDEILNAKLRGRGGAGFLTGKKWDLARSMKSRGPKYIICNADEG
                     DPGAYMDRSMLEGNPHAVIEGMMIAALAIGATHGIIYVRTEYPLAIKHATIALRSVRQ
                     LGLLGKNILGQGVDFDIEIFHGAGAFVCGEETALIKSIEGKMGEPRQRPPFPIQKGLF
                     GYPTCINNVETLSNVPYIINHGAAEYLKIGVENNAGTKIFSLVGKIKNTGLVEVPLGT
                     PIRKVVYDIGGGPSGEAKIKAIQTGGPSGGCIPASMFDLNIDYDSLAKAGSIMGSGGM
                     IVMDQNTCMVDVAKYFMSFLKDESCGKCFTCRKGTQRMYEILEDITEGRGTPGHLQLL
                     EELAQTVKDTTMCGLGQTAANPVLSTLRYFRDEYERHIADKRCEAFACKELTGAPCQA
                     ACPVDTEPWRYVALVEKGDYEEAYRVIRSANPFPAVSSRVCDRKCESRCNLGVSGGES
                     IGIRAIKRFVTDRVDPSAYRPKPAKNKGKEVAIIGAGPAGLTAAHNLSILGYKVTVFE
                     AEQVPGGMLSCSLPAYRLPRDIVEKEIQTLLNGNIKIEYGRTLGKDMSLQDLLSKKFS
                     AVFLSIGAHESWRLDLENEDIEGIYPSIQFLKAFNIRGEQLARGHVGIIGGGNSAVDA
                     ARVALRQKDVTSVSLFYRRTREEMPAYEEEIDAALEEGVRIETLISPVKIHVRQEEID
                     AALREGVELETLVTPVKIYSKEGRVVGIECIKNRLGDVDATGRRKPVPIPGSEFKVEL
                     DTLIVAIGEKPQSEFLSAMGLDVDKGGRIRVNPKTFETSLKGVFAGGDLVTGPNTVID
                     AIAAGKKAAQCMDRYLQNQPLVVPASPRRPGVFIEPAVIDEAEAQTATRAEPAVLPPS
                     QRLKNFREVTMVLPEEQARSECRRCLRCDLEFTKKQSAEKPAAAAIGGGKNG"
     gene            24174..>24836
                     /locus_tag="PLQ77_03865"
     CDS             24174..>24836
                     /locus_tag="PLQ77_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPL09846.1"
                     /translation="MVNLTLNGLSISVERGTTILEAAKFYGFPIPTLCHKEGLSPYGA
                     CRLCVVEIGEGPGAKLVSSCTYPVEEGMKVRTASERVLKARRIIIELLLASCPQSKVI
                     QDIASRHNVRQQRFRQEHEDCILCGLCVRMCNEQMMAGAIGFRGRGKTRSLGTPFDMK
                     SDVCRLCGGCMEVCPACQARCTFNQPEKAICGGCANLAPPCIAKDQFHDMMCYMSPCV
                     ACE"
BASE COUNT         5385 a         7005 c         7092 g         5354 t
ORIGIN      
        1 ccgcgggcgt ccgcgttttg ctggattcga cggacggctc cgagctctgg agcacgacgg
       61 gcgtttccga aaacatcatc gaggcgagct ggcaggcgct catcgacagc atcgagtaca
      121 agctcagcaa agaccggaaa aagaggtgac gaccgctgat tttttggccg tttgcagcca
      181 ttgctaaatc tttcaattgc tgatatggaa gcccaacgta aagcgagggc tcgaacggtg
      241 ttgtccataa acagccagaa tccccagatg cgcctcattc gaaaggcgac ggatgtgttg
      301 agggacggcg gcattatcat ctacccgacg gatacagtgt acggcctggg ctgtgattta
      361 tcgaacaagc gaggcattga gaaaatatac gagattaaaa gaagaaacaa aaaacgccct
      421 ttaagttttg tctgttccga tctgaagcac atcagtcagt acgcgctggt cacggattat
      481 gcttataaaa ccatgaagcg gtttttgcca ggaccctaca cgtttattct ggaagcatcc
      541 cgtctggttc cgcggatcat tttgccgaaa aggccgacga cgggcattcg cgttccggac
      601 aaccagattt gtcttgcttt gatccgggag ctcgggcagc cgattatcag caccagcgtt
      661 cagaccctgg acggggaaga cctcggcgat cccgtgatca tcaacgaaca cttcgggcgc
      721 gtggtggatc tgattattga cggagggatc atcgttcccg agccgtcgag cgtcgtcagc
      781 ctggtggacg accggattga agttcttcga actggaaaag gcgacgtgag cgcctttata
      841 taaaatacac gaagcggcgg gatgtcatgt ccgtcagccg cgcggcccgg gaaaggccgc
      901 ccaaaaggat ccttatgaaa catgtaatgc tcgttaacgc cgtgcacaag gagcaaatgc
      961 gcatggccac ggttgacgaa aaaggcaagc tgattgattt taacattcaa atggccgttc
     1021 gggaaccgat caccgggaac atttacaaag gcaaggtgct gaaggtcgaa cgcggcctgc
     1081 aggcggcttt tgtggattac ggcggcgtca aggacggttt tcttcccctg cgcgacgtca
     1141 gccacgaata cctgaccgaa ccggaaaacg gttcctccgg cggcagggcc gttctgaaga
     1201 cgggccagga aatcatcgtg caggtggtcc gggaaatcat cggcaacaag ggcgccctgc
     1261 tgacgtcgta catctccctg cccggacggt atctcgtgct gcttcccaac aagctctgcg
     1321 gcggcatctc ccggaaaatc gaaagtgagg aagaccgccg gaagatcaag acgctgatgg
     1381 accagatcaa ggttcccgag gggatgggtt ttatcgtccg cacggccggg atcaaccgaa
     1441 ccaagcagga aattcagcgc gactaccagt tcctgatgcg cctgtggaag gagatcacca
     1501 agaaggccga agacgcctcc gaaccgtctc tgctctacca ggagacggat ttcggcgtgc
     1561 ggtcgctgcg cgactacctg acgcaggaaa tggacgaaat ctgggtggac gaggtggaaa
     1621 ccttccgcaa gatgcgcgcg tacctgaaag cggtcgcccc ccgccatttg cgaatcctga
     1681 agcagtacaa ggacaagact ccgatttttg agcgcaactc gctggaagag cagatccggg
     1741 tgatttacca ggaacgcgtg gagctcaagt ccggcggcta catcattatc aatcccacgg
     1801 aagccatgat caccatcgac gtcaattccg gacgcgggtc gaacaagcga aacatcgaag
     1861 aaaccgcttt ccggacgaat gtggatgcct gtgaagaaat cgcgcgccag ctcaggctgc
     1921 gcgacctggg cggtctgatc gtcatcgatt tcatcgatat gatcgacaag aagcacattg
     1981 ccgaagtgga aaaggccttc aagaaggcgc tgagcatgga tcgcgccaga attcagcttt
     2041 cgcgcatctc caagttcggc ctgatggagc tgtcccggca gaaaaaacag tccacgattc
     2101 aggaaatcag ctacacgacc tgtccgtact gcgccggcag cggtctgcgc ccctctctgg
     2161 agtacgcctc tctcggggcg tttcgcaaaa tcgaatcgga ggcggtcaag ggcacctatt
     2221 cggagctgag ggtcagcctg ccgcatgaaa tcgccctgta catcctgaac aacaaacgga
     2281 ccgagctttt gaagctggag tcaaccttcg gcgtctgcct gattatcgaa ggaaaacccg
     2341 atatggcgtg ggacaatttc gaaattcagc agtccatgaa ggagcaggcc ctggaagtca
     2401 gcgccattca gggagtgccc ctgctgaacc ttgcggaaaa gacggagccg gacgcggagg
     2461 gcggtgaaaa gccggaagcg gaagccggcg aagccgaaga gccccccagg aaaaaggggc
     2521 ggcgccgttc ccgccacaag aaaagaaaat cgccggaggc attaaaaggc tccgttgcga
     2581 cggaagacgc tttgcccgac gcgcagcctt cccccggcgg gatggcgcct ccggtttcct
     2641 cctcgcagtt ttcgcgtttt cccgtcaagc cgctgaagat tgtcggcgcg ctgccggaag
     2701 aattctttcc ggtggactta agcggcgatc ttttcacgcc ggaaatggat caggaagata
     2761 cgggcccgga gcaggaggat aactgaatga cccttcgaag acttcccgaa ggcccggacc
     2821 gctccgtctc tgcgtcaggg aggacagcgc atggcggatg aaatccgaaa ggggcttttc
     2881 cggatctccg tgccgttgcc gaacagtccg ctcaaggacc tgaactccta cgtgatccgg
     2941 ggcgaagacc gcaatctgat catcgacacg ggtttcaacc gaaccgtctg ctacgaagcg
     3001 atgcaggcag gcctggccga actggggatc gatctgaatc agaccgattt catgctgacc
     3061 cacatgcatg ccgaccacac cggcctggtg acgaggctgg cgacgaaaac cagccgaata
     3121 ttcttcggca ggatcgacgc gcgcgttttt gacggcgatc acggctggca gtccctgatt
     3181 gacttcgccg ccctgaacgg ttttcccgtc gacgagcttc agaaggcgct cgccagtcat
     3241 ccgggattca aatacagtcc gcagcaaaag cctgtcttta ccctgctgga cgacggcgac
     3301 gtaatcgaat gcggaggcta ccggctgcga tgcgtcgcca cgccgggcca cacgcagggg
     3361 cacatctgcc tgtatgagga ggatcagaga attttctttt ccggcgacca cgtcctgttc
     3421 gacattacgc cgcacatcga gtcgtgggcc tatgagacca attcgctttc ggactacctg
     3481 gccagcctgg accgggtgta tgccctgccg gtggaactgg tcctgcccgg acaccggaat
     3541 tttcaggggg atctgaagga caggatccgc gagctgaagc atcaccaccg cgaacgggcg
     3601 gatgaagtgc tggaggttct gggcggccgg gcgatgaatg cctatgagat tgccgcgggg
     3661 atgacctggg acatcgactg cgaaacctgg gaggattttc cgattgccca gaagtggttt
     3721 gccaccgggg aagccatcgc gcatctgcgc ttcctggagg gagaaggccg gattcaccgc
     3781 cataccgatc aggcggttgt agccttttcg gccgtccgtc cctgattcgt cccgttcagg
     3841 atgccggcga tggtctgccg cttcatttca aatcaaacca aagcggggaa cggtctgcaa
     3901 ccgttccccg ctcgatgacc tgtcagaaaa aaaccatggc cagcgcttcc gcgacgcacg
     3961 ccggtttgtc ctgcccttcc atttcaatgg tatgcgtctg gcggatcaaa agacggttgc
     4021 ccggcttttc ttccacgccg gacagcacga tccggtcgcg gatgttcgat cccgaaggaa
     4081 cgggattgag aaaacgcacc ttgtccagac cgtagttgat ggacatcttc gcgccttcga
     4141 tcttcacggg cgcctggtag ctgaaaaaag ggatgtgcga aagcgtcagg aatccgtgcg
     4201 cgatggtccg gccgaacggt ccggcggcgg ccttttccgc gtccacatga atccactggt
     4261 ggtcgccggt gctgtcggcg aactggttga tctgcgcctg cgtcacctgt ttccagccgg
     4321 aaacaaaaac ttccttcccg aggtgcggct gcaattcttt tagtaccgat tcgatcgaca
     4381 tattttcccc tccttgtccc ggatcttcgg cccggccgtg ccggcctgcc tgctcgaaaa
     4441 gcgggccgcg gggagcgccc gcgaacccct tgccgcgttc cgtcctgcgc gctactccag
     4501 cgccgcgttg actttggcct gtgcggcttt tgcttctttc atgatgcgtt caaatagctc
     4561 ggcaatggtc ggcacgtcat gaatcaaccc ctgcgactgg ccgaccggga gcacgcccgt
     4621 tttggaatcg ccgtcttccg tcgcgacgcg aatggctttg aacgcgttgg ccatataggc
     4681 gagctgtctg gcattcttgt agccggacag cagaaccccg ataaaaagtt taaaatacgg
     4741 gatgcccatc tgcttcgcga tagcctgcga attgaaaaaa gcggcgggcc agttcaggcc
     4801 ggttttgacg gcttttttgg ctgcgtccgt tttcatgacc cggcacagga tgccgtcgaa
     4861 gcgcttggaa aacagcgtgt catagacgtc tttttcctgc gtcatccgtt tgtagttttc
     4921 atgcagcggg ctttccctgg ttgtcatgag tcttgttccc atggcgacgc cttccgcacc
     4981 cagcgcgagc gcggccgcga ttccacgtcc atcggcaaat ccgccggcgg caatcacggg
     5041 aattttcaga ttttccgcca gatgcggaat caaaaccagc gatgtgacgt cttcgccatg
     5101 cgccgccgct tcctgtccgg tggcgatgac cgcatccgtt ccgaaatcct gcgcgcgttt
     5161 cgcgtgtttc agattgacga cggtcgcgaa gaccttgccg ccgtatttgt gcgcttcctt
     5221 cacaatccag tcgcctttgc ccagcgcgaa attgatgacg ggcacttttt cctgcagcag
     5281 aactttggcg ttttccaccg cgcccggaaa catcagcgat gcgttcgcgc cgaagggttt
     5341 atccgtcagt ttccggattt cgcggatggc ttcccgggtc tgcgcggcgt tcagcggacc
     5401 ggtggccaga atgcccagcc ctccggcgtt ggataccgcg gccaccattt tgggaacgct
     5461 gatccagctc atgccggaca gaacgatggg atacttgatg ccgaccattt cagtgattcg
     5521 tgttttcata gttgctcctt ctatcgtgta gtttggtttg aaagcctccg gttcagtttc
     5581 cgcacagctt tgcggttctt gcggtgatct ctttggcctg ggctgccatt ctttccagca
     5641 gctccgcgca ggtggggata tcgttgatca ggccgatgga ctggccgacc atcaaaggcg
     5701 cgtaatcaac atcgccggtt tcccaggctt gcttgacttt ctggccggac aagagcggaa
     5761 tcagctcttc aaacccgccg ttgcgggctt cgatagccag gacttcttca atgaccttgg
     5821 atttcaatcc gcggccctgc aggccgatgg attttccgta aatcaccgtg tcaaattcct
     5881 gccggcggat cagttcctgc ttgatgttgt catgcacttc acattccttc gtcgcgatga
     5941 agcggctggc catcatcacg ccctcggcgc ccagcgccaa agcggcggcc atggtccggc
     6001 cgtccgcgat gccgccaacg gtgaccacgg gaatttttac cgaagccgca atcttgggtg
     6061 tcagcaccat ggaggtgaca tcgtcattca acgggtgacc gccttcttcg atgccggcgg
     6121 catagattcc gtcaaagccg attttttccg catgcagggc atggcgcaaa gcgccgacct
     6181 tatgaaacat tttcacgccg gctttcttga gcatctcgac ggcctccatt ccggccacct
     6241 tgtccagcgg cgtgccggaa aactcgatgc ccgccacttt ttcctcggcg caaactttaa
     6301 catacatctt gtggtgctcc ggcgtgatcc ggaccgaggg cagcagcgtg atggccacaa
     6361 agaagggttt atcggtgagc cggcgcgttt cccggatcgc cgcgcgaaat tcgtccgccg
     6421 tttcataatt gcccgccgtc agattgccga tgccgccggc attggacacc gccgcgcaca
     6481 tttttggttt gcaaagccac atcatggccc cgcagataac gggatacttg actccgaaca
     6541 tcctggtgat ttgcgtgtcg aacatgaaat acctcctatt tttttccgtt gtttccggtc
     6601 tttcgctttt tggccgcctt caggctgctc tgaatattct gctccacctg cttctggaaa
     6661 agacgccgaa gaatgtagtc ccggtagaaa tttccgacct gcgtcagttc ggcggtaatg
     6721 gcgatgtttt cccgcgtcga ttttccggcg accatcttgc ggcgaagcgc catctctttc
     6781 tccacgattt gccttcccag ctccacccag aagaccagct cctgaagctt catccccaga
     6841 tcggccatct ttttgatgcc gtcccggccc gcgcggacgt cgtccggatc atacaggggc
     6901 ttgtcccttt cgaacgtgaa cgggaccagg atgcccagac gccgggcctg cgccaattcc
     6961 ttttccgtaa ggcccgtcgc gtcaagaaaa gcggcctggc cgatgagccc gttttccgcc
     7021 tgttggccgg agcggaacac ggcgttttcg atcgtttcgg cttcggtcag gttcaggcct
     7081 tcatccattc gacgcagaat gtttttgatg aggtacagag gaaagtagcg tctttccttc
     7141 agctcctgaa tgacgcgcag gcgctcgatg caggcctcgt cgtaataggc cattttctca
     7201 ttcgcttttt cgggtttggg caaaagcccc tctccgacgt aatagcggat ggtcgagggc
     7261 cgcacactgc ttcttttcgc gatttcgccg attttcaatt tcattcactg caccaaacat
     7321 taaagttgag gtttaatgtt atagtgatat tgaagacggc tgtcaagcaa attgtcaaaa
     7381 agacaaaaac caagtgaaaa caaaaagaaa gcccgcctgc tcggctcttt ggaatgaaaa
     7441 tctcgaggcg gccggcaaag atcattgctt acaacagggc ggcttttatt tctttgacat
     7501 gcgcggcagc cgcccgaatc ccgtcgccgc cgttttggtt ttccccgatg agtttgacga
     7561 cggagctccc gatgacaacg ccgtcggcga ttcttgctat ggccctggcc tggccggcgc
     7621 tcgtgatgcc gaaacccacg gcgaccggca gtttggttaa tttgcggatt tttgaaatct
     7681 cgcgggcgat gtcttctgtt ttcggagcgg cggtgccggt gacgcccgtg atggagatgc
     7741 agtaaagaaa tcctgaagcg gctttcagaa tggctttcaa ccggtcgtcg cccgtggtcg
     7801 gcgcgacaag cgaaatgaag tcgattccgg ccgcattcgt aaaaaccctc agttcgcccg
     7861 cctcctccgg cggcaggtcc acgacgagga tgccgtccac ccccgcatct tttgccgctt
     7921 tggagaactt ctgggcgcca taggcaaaga tcggattaaa gtagccgaaa agcacaatgg
     7981 gaatccggga tgccctgcga atatcggcca ccagatccag aacgcctcgc agtgtggttc
     8041 ctgctttgat ggcccgctgg gatgcggcct gaatgacggg gccgtcggcc gtgggatcgg
     8101 aaaacggcac gccgatttcc agaatatccg ctcctgattt ttccagactc aacgccagtt
     8161 cgcgcgtcgc cgcgatcgac ggatcgcccg ccgttgcata aacgatcagc gccttttctt
     8221 ttttggcgcg cagctcttca aattttttcc caattcttcc catattttcc tcttttcatt
     8281 ttcgctccct ccttcctgcc gggcggggac tgaagggagg ggaaataatt cattttcacc
     8341 ctcacccagc cctctcccat caagggagag ggcctcgcag ggaacgctcg cgaccaccca
     8401 tgcgggcggc aagacggttc cctacagcgt ttccatgatc gtccgggcgt ccttgtcgcc
     8461 gcggccggaa aggcagatga ccaaaatccg gcgcttcttc attttcggag cgattttcat
     8521 ggcgtgggca atcgcgtgcg aactttccag cgccggaatg atgccttcct gccgcgacaa
     8581 atacaaaaac gctccgatcg cttcctcatc cgtcacggtg acatacgtgg cgcggccgga
     8641 ttgtgcaaaa taggcgtgct ccgggcccac gccggggtaa tccagtcccg ccgcgatgga
     8701 gtaggcgtcg cgaacctggc cgttttcatc ctgcagaagg taggtcttgt tgccgtgcag
     8761 gacaccgatt tttccgccgt tgatgcttgc cgaatgctcc agcgtattca tgccgcggcc
     8821 ggctgcttcc acgccgatca tcttcacctt cttttcattg cggaagggat aaaacaggcc
     8881 catggcgttg gagccgccgc ccacgcaggc caccagaaca tcgggattcc gtccttccgc
     8941 tcttgccgtc tgctttttga cttctcgccc gatgaccgac tggaaattgc gcaccatcat
     9001 cggatagggg tgcggcccgg cggttgttcc gatgatgtaa aacgtgtcgc ggacacttcc
     9061 cacccaatag cgcatggctt cgttcatcgc gtccttgagc gtggccgtgc cgctttggac
     9121 cggaaccacc tcggcgccca gaatcttcat gcgaaagacg ttgggcgcct ggcggcggat
     9181 gtcttcttct cccatgaaga ttttgcattc cattccgaaa agcgcggcca cggttgcggt
     9241 cgccacgccg tgctggcccg cgccggtttc ggcgatgact tttttcttgc ccatgcgccg
     9301 ggccagaagc gcctgcccca gcgtgttgtt gatcttgtgc gagccggtgt ggttgagatc
     9361 ttcgcgtttg agatagattc tggcgccgcc aagcgcctga gtcatccggc aagcaaaata
     9421 aagcggcgtc ggccggcctg cgtagtctcg ccggtaccgg tcgaattcct tctcaaagga
     9481 tttgtctttt tgcgccatgg cgtaagcttc ttcgagctcc agcagagcgg gcatgagcgt
     9541 ctcggccgca tagcggcctc cgaaggcatc aaagtgtcct ttttgatccg gcagcatttt
     9601 catgttctgt cccttccaat aaaaatattc agcggtgttt cgccggctga cgcggaatga
     9661 atgatccgca tcatgcgcgc gatttttgcg gagtcttttt tgcccggctt ctcttccgcg
     9721 ccgctgttga tgtccagtgc ggcaggccgg accacctcaa gggctgcagg aacattgaat
     9781 tcgttcaagc cgcccgataa aatcagcggc ccggcaatct tcctggccag gtcccagtcg
     9841 gccgtcctgc ccgtgccgcc gtaaagcccg ttgtggcgcg catcaacgag aatcgcggcg
     9901 acatcaaatg cttcagactt ccggacatct tcttcggttt gaagaaccag cgccttgaga
     9961 atccggtttt ttggaaattg ccggcagtag ccgggggatt cgtcgccgtg gagctgaatc
    10021 atgtcgaggc ggcagtgctc aaacgtgtgc cggacaaagg attctttttc attgacgaag
    10081 acgccgacct tcaccagccg gtccggcagc gcttttgtga tctgtgccgc cttttgcggc
    10141 tcgatgaagc gaggggaagg cggatggaaa ataaaaccga ccgcgtctgc gccgttttca
    10201 gccgcggtga gtgcatcttc aaggtttgtg atgccgcata tttttacctg catgacgatt
    10261 ccctccccag aagcgagcgc agtgcacggc cgatgtccgg agcggtcatc agcgcttcgc
    10321 cgatgagaaa agaagaaatg cccgcgttca tcaggtgttc gatatcggcg cggtttttca
    10381 gagcgctttc cgcgacgacc gtgcggtcgc cgggaatctg tttcctgagt cggcaactgg
    10441 tctgaatatc cgtcgcgaac gtatcgagat tgcggttgtt gatgccgatc atctccgctt
    10501 ttgccgcgag tgccgcctgc agctcctcct ctgtgtgaac ctcgaccagc gggcacaggc
    10561 ccagtgcttc ggaaagggcg atgaattcaa aaagccgcgg tcccagcgcg cggacgatca
    10621 gcagaatcgc gtcggcgccg acggctctcg tttcgtaaac ctgaagcgga tcgatgataa
    10681 aatccttgcg caggacggga agacgaactt cacgccgaac gctcgtcaaa tcgcttgtgt
    10741 ggcccaaaaa ataggtttgg tcggtcaaaa cggaaacggc ggccgcgcca tttttctcat
    10801 acgtttttgc gatcgccgcc ggatcgaaat gctgcaacat gcgtccgcgg gaaggcgagg
    10861 cgcatttcac ttcggcgatg acggcgcagt cggcaccgcc gagagccgcg cggaaatccc
    10921 ggcagggcgg cagcgctgcg atggcttcgc aaagacgctc agtcgtcgcc tcctttttga
    10981 gacgggccac ttcctctttt ttggtttcaa cgatcttgtc taaaatcatg tcgtcctctt
    11041 tttttctccc cttttttcac cctctcccta accctctccc atcaagggag agggaatgaa
    11101 cctgcaattc atgatcgccg ccgctaaacc ctaacattct cccgtcaagg gagagggaat
    11161 taaccgttcg tcatctcgat gagcgcccgc agtatttttg ccgcccggcc gctgtcgatg
    11221 cagtcggcgg cgattttaac gccctcctga atattgcctg ctttctcacc ggcgacaatc
    11281 gccagcgccg cgttcagtaa aacgacatcc cggcaggcgc ccggctttcc ggagagcacg
    11341 tccgtcagga ttcgggcgtt gacctccgga tcgcctccgc ggatcgcctc gagcgggtga
    11401 gtggttccga cgaactcctt cggggaaagg ttgtaggtgc ggatgatgcc gtctttgagc
    11461 tccgccacgc gggtttcccc ggtgaccgtc acttcgtcga ggccgtcgac gccgtgcacc
    11521 acaaaagccc gcttcgtccc catgtttttc aaaacgccgg cgaacatttc ggcaagcttt
    11581 gcgtcgtaaa cgcccagcag ctgcgcggtc gcgccggccg ggttggtgag cggccccagc
    11641 atgttgaaaa tcgtgcggat gccgatttca cggcgcgggc cgatggcgta cttcatcgcg
    11701 ccgtgcagcc tcggcgcgaa cagaaagccg atgccgatct gctggagaca ctcttcgacg
    11761 atttccggat cgatgctgat attgacgccg agagcctcca gcacgtcggc gctgccgcac
    11821 ccgctggaga cggcccggtt gccgtgtttg gcgaccacga tgcccgccgc cgccgcgaca
    11881 aaggccgtcg tggtggaaat gttgaacgtg cccagcttgt cgcctccggt gccgcaggta
    11941 tcgacgacgg ttgtcgcgca ggcgttcacg cgcgcggcct tccggcgcat gatgcgcgcc
    12001 gcgccggtca cttcttccac ggtttcgccc ttcatgcgga gcgcggtgat gaacgagccg
    12061 atctgcgcgg gagtggcttt gccctccatc acttcttcca tgacggccat catatccccc
    12121 tccggcagat cgatgttttg tactaccttg tccaaagctt ctctgatcat gatccatgct
    12181 cctttcggcc gatctgtttt aaaaaatttc gaaggattct tttcccctgc ggcgtaagga
    12241 cggattccgg atgaaactga attccttcca cgggataatt tttgtgccgc agtcccatga
    12301 tttcaccctc gggggtccgg gcgctgattt caaggcaggg cggcagggat tccggccgca
    12361 ccagcaggga atgataccgc cccgcggcaa agggttgggg cagccccgca aaaatcgttt
    12421 tgccgtcgtg ctccacgggc gataccttgc cgtgcatgat gcgttcggcg cgaacgactt
    12481 ctccgccgaa cgcggcaccg atgcactggt gtcccaggca gatgcccatc agcggcacgc
    12541 tcgaataaaa tttctgaatc acgtcgatgg tgacgcccgt ttcccggggc gtgcatgggc
    12601 cgggcgataa aaaaattgct tcgggtctct cgcgccggat ttcttcgagg gtgatccggt
    12661 cgttgcgccg gacccggacg tcctccccca tctgcctcag gtattgaacg atgttgaacg
    12721 taaaggaatc ataatggtcg atcatcagaa tcatagagcg ctcctgccgt tttcgagaac
    12781 gaatccgctt gaggcgagct ccacggcctt ctgcatgccc cgcgccttgt ggagcgtttc
    12841 catgtgttcg gcttcgggcc tggaatcatg gacaatgccc gcgccggcct gaatgaaaat
    12901 ccttccgtcc cgaataacca tcgtgcggat ggtgatgcac agatccatgt tgccgctgaa
    12961 gccgatatag ccgaccgcgc cgccgtacac gccccgccgc acgggttcga gttcgtcgat
    13021 gatctgcatg gcccggactt tgggcgcgcc ggagagtgtt cccgccggaa acgtggcggt
    13081 cagcacgtca aaggcgtccc tgccgcaatc gagctcggcc cgcacattgg ataccaggtg
    13141 catgacatgc gaatattttt cgaccaccat gtactggttg acctgcaccg acccggtttg
    13201 ggcgatcctg ccaaggtcgt ttctgcccag atcgaccagc atgacgtgtt cggcccgctc
    13261 cttttcatcg gagagcagct cgtcggccag cgcgcggtcc tcctgctcgt tttttccgcg
    13321 cggccgtgtg ccggcaatcg gcctcagctc cacttcgttt tgctccaggc gcaccatgac
    13381 ttccggcgaa gagccgatca gggtcagatc atccagcttg aggaaaaaca gatagggcga
    13441 cgggttcacg cagcggagcg cccggtaaag atcgaccgga tcggccgtgc agtccgtgga
    13501 aaaacgctgg gagagcacca cctgaatggc gtctcccgcg gcgatgtagt ctttcgcttt
    13561 ctccacgatg gccttgtacc ggtcgggcgt catattggat tcaaaaacca cgacggaagc
    13621 tttttgggcg ggagccggat attctgccgg cgtctgcaac agcgcaatca gatcatcgat
    13681 ctttcggcac gcggccgcgt acgcttccgc gcgggaatcc tcgtcttccg caaaggcgca
    13741 ggcgacgacc ttgatcgtat ggcgcacatt gtcaaagata atcagggaat cgctgatgac
    13801 aaaaacggat tcgtccagat tcaggtcgtc gggcggcaca tcgggaagct tctcgaagta
    13861 gcgcaccgtg tcgtagccca gatagccgac cgctccgccg aaaaaacgcg gcagtccggg
    13921 agggacaacc ggccggtagc ggcccagcag ctcccgcatt cggcccagcg ggtctccccg
    13981 gtgttcggtc tcgaagattt tccccttttc ttccagaatg actttcgggc ctcggattct
    14041 gaacacgatt tccgcattag ttccgataaa ggaataccgc ccccactttt cgcctccttc
    14101 aacgctttcc agcaggtagg catgccgttt gtgccgaatt ttttttaaga cggaaacggg
    14161 ggtttcgaga tccgccagga tttcccggta aacgggaatc aggttccccc ttttggcgca
    14221 gacttcaaaa tccgcaaatt ttggtgaata catgggcgct ccctttaaaa aataaaaaaa
    14281 ggccgtgcgt tgcgacacgg cctttttggg ttgaaccata caaaaaagcc gcggagtcct
    14341 gagaggtctc cacggctttg acttttatct aataataaag atcagcggcg gatgctcctc
    14401 tccgggaggt tccaccacca ccagttgttg attgtatgtg cgttgaatct cttttgcatg
    14461 ctgcaccttt ttctcgcttg ttggttgctt ctataacagc aggtccgttt tctgtcaaac
    14521 gttttttgcg ggcgcgggtg cgcgagaaca ttcacgccct ggttttttgc ggaattttct
    14581 ttttgttccg gcgttttgac ggtttgccaa gggggcagac ggaaacgcag atgccgcaaa
    14641 gatgaacgtt ccggttgagc ctttcctgcg acaacctgcg gcaggcctcc atgcagcgaa
    14701 aagcgttgta aaactcgtcg cggtcaagag tcgaattcca cagcttgccg ttggccgcct
    14761 ctccggggca cagcctcacg cagatgtcgc aggcgccgca gcggctttcc gtgatgggtg
    14821 tgccgcaagg ttcaatgggc tgatgggtaa gaaccgtcgc caggcgaatg cgggggccga
    14881 acgctttcga caccagcaaa tccgttttgc cgatccagcc cagaccggcg tgggtggccg
    14941 ccattttgtg ggagaagttc atccgcaagg ttttgccgaa gtccgggggg atgtcttccg
    15001 cccgcacggt ggctttgatc tgcaggcagg aaatgccgtt ttgcgtgaga aaccgcgata
    15061 ttttcgccgt gatcccattg agctcatcat tggtggaatt gtataattcg taataaggca
    15121 tggtcgggcc gtcttcgatt tcatcgacaa tcgcgtcgtc cagccttttg ccgacaacaa
    15181 cggcataacg gtacggataa tacccggcca tcagatcgcc catgtcggca aatccgacga
    15241 cataattttc ccgatcgggc agaaggctga tgatttcgtt ttgcagcgtc atggcttcac
    15301 gatgagccat tttgcctttc tcctttagtg aaggttcaag aatcctatca catgccttct
    15361 tttcaaaaca gagcaaattc ttttgccggt gcttgcggag aaaaaacagg cgctgcagga
    15421 gcgaacgcag aaagggtaaa gattgacggc gtcttttgct tttgttatgg tgtcgcagtg
    15481 ccggttgctt ttggggaaat gcggttgccc ggcacggagg cctcgaatgg acatcattgc
    15541 tctggtcatt ctgatatctt tgctggtcat cattgtgctg ctgctctggc agctcaggcg
    15601 gagttccgtt gattggacgc cgcttgtggg caaactggat gccttgcggg atgcgcagga
    15661 aagaaccgac cgttcgctgc gcgacgagat tgcccggtcg cgctcggagg cgcagtcgaa
    15721 cgcgcagcag gaacgagcgg agctcggggc ttcgctttct tccttcggcg actccgttca
    15781 ggcccgcatg accgatattg ccaaaatcca aagcgatcag atggagagct ttgcgcggca
    15841 actgtccctg cttgccgccg gcaatgaaaa aaagatggaa gacattcgtc tgatggttga
    15901 cgaaaagctc aggcaaatcc aggaggacaa tgcccgccag ctcgaccgga tgcgcgagac
    15961 cgtggatgag aagctgcaaa gcacactgga gaagcggctg ggagaatcct tcaagcaggt
    16021 ttccgagcgc ctcgagcagg tccatcaggg gctgggcgac atgcgcacgc tggccgccgg
    16081 cgtaggcgat ttgaaaaaag ttctcaccaa cgtcaagtcg cgcggcacct ggggggaagt
    16141 ccagttgggc gcgcttctgg aggagatcct gtctccggat caatatctga agaacgtcag
    16201 gattcaggaa tccggcggcg attttgtcga gttcgccatc aggcttcccg gccagggcga
    16261 ttccccgacg gaaagcgttc tgattccggt ggatgcgaaa tttccggtgg aagactactc
    16321 ccgtttgatg gaggctcagg aaagggccga tgcccccgcc gccgaagatg cggtccggca
    16381 gctggaagcg agcattaaaa aggcggccag agacatctct caaaaatatc ttgctcctcc
    16441 cagaacaacg gacttcggca tcatgttttt gccttcggaa ggcctttatg cggaagtgat
    16501 ccgccgtacc gaactggtgc agcacctgca gcgcgagtac cgcattgtga tttccgggcc
    16561 ttcgacgttt gccgcttttt taaacagcct ccagatgggc ttccgcacgc tggcgattca
    16621 aaagcgctcc ggcgaggtct ggaaagtgct gggcgaggtg aaatcggcgt tcgggcgctt
    16681 cggcgattcg ctcgatgccg tgcgcaaaag actggatcag gcttcaagct ccgtggatga
    16741 cgcgcagaaa aagacgcgca cgctggccgg taaattaaaa gcggtggaaa gcctgtcgga
    16801 cgcttcaggc gaaggcactg ttgcggatac cgaagcggat tgatgaggcg ccggaggcgc
    16861 gtcgattcat cccggtttta cgcatgggtg aagcatattt ttcttcctga gaagacaacg
    16921 aagcgcattt tctgaaggaa gtatgcgata agggtttaca tgctgaaaaa caaaagtaat
    16981 gtcgattcgt ccgctgcaag cgataaaacg cagttatatt taatggaacg cgtcaaagaa
    17041 ctgacgtgcc tttacgatat ggcgcggatc gccgccgaca caagtctgaa tattgaaaat
    17101 atgctgatgg gtattgttgg aatacttccg gaggcctggc aatatccaaa agatgctttc
    17161 gcaaaaatcg tttttaataa ccggattttt tcgatgccga attatccaaa atttgtccag
    17221 cggcagagat cagcgctggt catcaatggc aggcgtcgcg gttacgttga agtggcctat
    17281 ccaaagaagc gaggcgcgca aaatctgaac cttttcctgc aggaagaaca gaagcttctc
    17341 gacacggtgg cgcgggaagt ttccgccatg atccggcgca gagaaacgga acaggagaaa
    17401 ttaaaactgg aggatcagct ccgtcatgcg gacaggctgg caacgatcgg ccaactttcg
    17461 gcaagcgtgg ctcacgaact caatgaaccg ctcgggcaca tcctgggatt tgccgaactt
    17521 gccaagaaat gccccggcct tcctgtacag gcgatggccg acatcgagaa aatattagca
    17581 acatccctcc atgcccgtga ggttgtaagg caaatcctga ttttctcccg tcaggcttct
    17641 caacaaaaaa caaaagtgaa cctcaacaac cttataaaac aggggttgac ttttctggaa
    17701 tcccgatgtg tcagcgaggg aataaaatta cagtgtcgat tggggcttga tgtaccggag
    17761 attgacgcgg cacccgcgca aatgaaccag gtttttgtca gcctgatggt caatgccata
    17821 caggcaatgc ccgatggcgg aacgatatcc atccggacgc gcagggcagg ggagaaagtg
    17881 tctctcaata tagaggatag cggcgtcggc atgaacgaag agattctgca aaaggttttt
    17941 attccctttt ttacaactaa ggaagcggat gttggaactg ggttaggtct gccggtcgtt
    18001 catgatatca tcgccgccca caagggttct ataaaaattg aaagcaaagt cggaatggga
    18061 acgaaatgca aaatacaact gccggtctga aaaaaccggg cgcgttaaaa caaaaagaaa
    18121 aaaaacagcc ttccgataaa aaagatgaca tccttattgt ggatgacgcg ccggcaacat
    18181 tggaattgct ggagcgcaat ttgtcgtctg tcggatacgg gatatggatg gcgtctaatg
    18241 tggaagaggc gctcagggtg atcgacagct cccatattga tctggtaatt accgatttca
    18301 aaatgccggg cttaagcggc gttcatcttg cccgccatat tcgggaaaat tacaacgata
    18361 tggcggtgat gatcatcacg gcctatccca gcgttaaagg agcggtggag gcggttaaaa
    18421 caggcgcgga agagtacctt gcgaaaccct tcaccggcga agagctttta tccgccgtca
    18481 aaagggtcct ccggaatctc cacattcgca gagcggccca cgcctcgaac aagccgcgaa
    18541 tccatatccc tcacggtctg atcggcgagt ccggtgcaat gtcaaagatc agcaatttta
    18601 tcaacaaggc ggcgcgaaca tcggcaaccg ttttgatctc cggagaaagc ggaaccggca
    18661 aagaactcgt tgcgcgcgcg attcattata aaagcgagcg ggcttcagcg ccctttgttc
    18721 ccgtcaattg cggcgccatt cccgaggaac tcctggaaag cgagcttttc ggccacatca
    18781 aaggggcttt cacgggcgcc aacgaaacgc gcgccggttt ttttcaaaca gcggatggcg
    18841 gcaccatttt tctcgacgag atcagtgaaa caagcctttc catgcaggtt aaacttctgc
    18901 gggtcctgca ggacaaggaa gtttgcatgg tcggcgcgac acgcaccaga aaagtggatg
    18961 tgcgcatcct tgcctccacc aacaaaaata tacccgcgct cataaaaaaa ggccagttcc
    19021 gggaagacct ttactaccgg atcaatgtca tcaccgtgga attgccgcca ttgagggaac
    19081 ggcaggatga catctttctt ttaacgcgcc attttgccgc caaatttgcc gaggaactcg
    19141 ggcgcaccgc gccccggttt tccgatggcg ccttgcaggt gctgcgcagc tatcactggc
    19201 cgggcaatgt aagagaattg gaaaatatta tccagcgtct tgttgtcatg acggacgaag
    19261 acgtgatcga tgtcccggac ctgccctccc tgatgcggtt ttcagcccac cagcctgcgg
    19321 ctcccgaccg cacccttgcc gcggtggaag ccgaatatat tcgaagcgtg ctgctcagcg
    19381 tgagcaataa taaaaccctg gcggcaaaaa tcctcgacat cgacagaaaa acgcttcggg
    19441 aaaaactcgg gaaagcgaag ccgtccgcat cgaggtagat ctccccggcg gttcaaaatt
    19501 ccccggtttt aagcattgct ttgcgctttt ccttattcat tatttccccc atttccgttc
    19561 tgcggaatca ttctatctta agaatatcat tccggtaact ttatcggtaa gcggcaaatc
    19621 ccgtcgttaa aaataattgg caagcatgtt gctcttgctt cacaacgatc atctatcgaa
    19681 aggggaaaga aatgaaaagg accgcccgtg caagcgacga gatgaaaaag accgaaaata
    19741 ccaatgcccg gaatcaaaaa ggcatctgct cttcatgcga aaatttatct ctttgcagtt
    19801 ttattgagcc gcaaagacag cctgtcttgt tttgcgaaga attcagcatt ggcaaagccc
    19861 ggactgcgcc ggttgtatta gaaaaaaagt ccggctctcc ggccccgctg gagatcagca
    19921 tggggctgtg cgccacgtgt gatgatgcaa agacctgcct ttattcaaag gacgaagccg
    19981 gcgtctggca ttgcgaggaa taccggtgtg acggctgcgc aaaggaatcc ggaagtccgc
    20041 cgagtttgga aaaaagaggc atggatgcaa aagacatcct tgagacgatc aggaagcatg
    20101 acaaaaacaa gggggcgctg attgccatcc tggaggaaat ccaggcggcg tatacgtatc
    20161 ttccggaaga ggcgctcagg ctggtttcaa aagagaccgg ccgctcgctc gtggatatct
    20221 acggtgtcgc aaccttttac aaagcattca gtctgaaacc gcggggcaag cattgcgtat
    20281 ccgtctgcct gggtacggca tgccatgtcc ggggcgctca ggggatcgtt gacgaattca
    20341 agcgcgagct tgacattgcg cccggcgaaa caacggcgga caacgagatc accctggaaa
    20401 ccgtcaattg tctgggggcc tgcgctctgg gaccgaccgt ggtggtggac gggcattatt
    20461 tcccccatgt gagcaaaagc aaggtgaaaa acatcatcgc cagggcgatg gccggtctgg
    20521 aaaaaatcga tgcggaaacc gacaaccggg tattccccct ccaggtttcc tgctccgcct
    20581 gcaataaaag ccttctggat ccggagtatc cgatggacgg ttatccctcg attaacctcc
    20641 gtgtatcctg caacggcacg agaggccggc tgcgcctgtc ttcgctttac ggcagcgggg
    20701 cctcggtcct ggagcatccg attccgtcaa acagtctgtc cagattttac tgtccgcact
    20761 gcgcgggcga actgccgagt ttctctacct gcacggagtg cggttccccc atggccgcca
    20821 tgagggtctg cgaaggctgc gcctgggagg tatgcacgcg tcagggctgc gaaggccatc
    20881 tgctgaatct gaatcaaatg aacattcaaa aataacgatg aagggaaaag agatgaagag
    20941 gtttcaatct gtcgctcaat tcaagacgta tcgcaaaaca tttctggcaa cttcggatca
    21001 gtccaagccc tgcattgtca tctgcggggg caccggcgga caggccagcg gctcgaatga
    21061 tctgatccgg atcatcaagc gccaaatcct ggaaaaggat ctgcacgaca aactggacct
    21121 gcggattacg gggtgcctgg ggttttgcga aatggatcct ttcatcatcg tcgaaccggg
    21181 atacaatctg tacccgaagc tgaaaatggc ggacgttccc cggattatcg acgcggccgt
    21241 gagagggaag gtggtcgaag aactcctgta ccgggaaccc ggttcgacgg ttaaacgcta
    21301 ctccgaaaaa gaaattccct ttttccaggg acagacccgc atcgtgcttg gccagaacca
    21361 gcgcctcgat cccatccgga tccacgacta cctgaaaata ggcgggtacg ccgcgctgga
    21421 aaaagcgctg cagaaaaaag accgggcctg gctggtcgac gaaatcctca acgccaaact
    21481 gcgggggcgc ggcggggccg ggtttttgac cgggaaaaaa tgggatctgg cccgcagcat
    21541 gaagagccgc ggcccgaaat atattatctg caacgcggat gaaggagacc ccggcgccta
    21601 tatggaccgg agcatgctgg aagggaatcc ccacgccgtc attgaaggga tgatgattgc
    21661 cgccctggcc atcggcgcca cgcacggcat catttacgtg cgcacggagt atccgctggc
    21721 catcaagcac gcgaccatcg cgctgcgcag cgtccggcaa ctggggcttc tgggaaaaaa
    21781 tattttaggg cagggagtcg atttcgacat cgaaatcttc cacggcgcgg gcgcgtttgt
    21841 ctgcggagag gaaacggcgc tgatcaaatc catcgaagga aagatgggag agccgcgcca
    21901 gcgtccgccc tttccgattc aaaaaggcct tttcggctat ccgacctgca tcaacaacgt
    21961 tgagactctg tccaacgtgc cctacatcat caaccacggc gccgccgaat atttgaagat
    22021 cggcgtggaa aacaatgccg gcacgaagat attctccctg gtcggcaaga tcaagaacac
    22081 cggtctggtg gaggtgccgc tcgggacgcc gattcgcaaa gtcgtctatg acatcggcgg
    22141 cggcccttcg ggcgaggcaa agatcaaggc cattcagacc ggcggaccgt ccggcgggtg
    22201 cattccggcc tccatgttcg atctgaacat cgattatgac agcctggcca aagccggttc
    22261 catcatggga tcgggcggca tgattgtcat ggaccaaaat acctgcatgg tggacgtcgc
    22321 aaaatatttt atgagcttcc tgaaagacga gtcctgcggg aagtgtttca cctgccgcaa
    22381 gggcacgcag cggatgtatg aaattctgga ggatattact gaaggacggg gcacgccagg
    22441 ccatctccag ctgctcgaag aactggcgca aaccgtgaaa gacacgacca tgtgcgggct
    22501 cggtcaaacg gccgccaatc ccgtcctgag cacacttagg tatttccgtg acgaatatga
    22561 acgccacatt gccgacaagc gctgcgaagc gtttgcctgc aaggaactga caggggcccc
    22621 gtgccaggcg gcctgccccg tggacacgga accgtggcgc tatgtcgccc tggtggaaaa
    22681 aggcgattat gaagaagcct atcgtgtgat ccgttcggcg aatccttttc cggctgtaag
    22741 ctcgcgggtc tgcgaccgca aatgcgaaag ccgctgcaac ctgggcgtaa gcgggggaga
    22801 gtccatcggc atccgcgcca tcaagcggtt tgtcaccgac cgggtggacc cgtccgctta
    22861 ccggccgaaa ccggcgaaga acaagggaaa agaggtggcc ataatcggcg ccggccccgc
    22921 gggcctcacc gccgcccaca atctttctat tctgggctac aaggtgaccg tctttgaagc
    22981 ggaacaggtg ccgggcggca tgctgtcctg ttctctgccc gcttaccggc tgccccggga
    23041 tattgtggaa aaggaaatcc agacacttct caacggcaac atcaaaatag agtacggccg
    23101 gacgctggga aaagacatgt ccctgcagga cctgctttcc aaaaaattca gcgcggtctt
    23161 tctttccata ggggcgcatg aaagctggcg tctggatctg gagaatgaag acatcgaagg
    23221 aatctatccg tccattcaat tcctgaaagc gttcaacatc cggggcgaac aactggcccg
    23281 gggccatgtg ggaatcatcg gcggcggcaa ttcggcggtg gacgccgcgc gtgtcgccct
    23341 gcgccagaaa gatgttacca gcgtgtcgct tttctaccgg cggacccgcg aagaaatgcc
    23401 ggcttatgaa gaagaaatcg acgccgccct tgaggaaggc gtgcggattg aaaccctgat
    23461 ttcgccggtc aagattcatg tgcgccagga ggaaatcgac gcggcgcttc gggaaggcgt
    23521 tgaactggag accctggtca cgccggttaa aatctattca aaagaaggcc gtgtcgtcgg
    23581 cattgagtgc atcaaaaacc gtctgggcga tgtggatgcc actggaaggc gcaaacccgt
    23641 tccgattccc ggaagcgagt tcaaggtgga actcgacacg ctcatcgtcg ccatcggcga
    23701 aaagccccaa agcgagtttc tgtccgcgat gggcctggac gtggataaag gcgggagaat
    23761 cagggtcaat cccaaaacct tcgaaaccag cctcaagggc gtttttgcgg ggggcgacct
    23821 cgtaaccgga cccaacaccg tcattgacgc catcgcggcc ggcaaaaaag ccgcgcaatg
    23881 catggatcga tatttgcaaa accagccgct ggttgtgcct gcctcgccga gacgtcccgg
    23941 cgtctttatc gaaccggccg ttattgacga agcggaagcg caaacggcaa cccgtgccga
    24001 gcccgccgtg ctcccgccgt cccaaaggct gaaaaacttc cgggaagtca ccatggtcct
    24061 gccggaagag caggcccggt cggaatgccg gcggtgcctg agatgcgatc ttgaattcac
    24121 caaaaaacaa tccgcggaaa agcctgcggc ggccgcaatc ggaggtggga aaaatggtta
    24181 atctgacact gaacggactg tccatatccg ttgaaagggg gacgacgatt ctggaagcgg
    24241 cgaaatttta cggtttcccc attccgacgc tgtgccacaa ggaggggctc tcgccttacg
    24301 gcgcgtgccg gctttgcgtg gtcgaaatcg gcgagggccc gggcgcgaag ctcgtctcgt
    24361 cgtgcaccta tcccgttgaa gaagggatga aggtccgcac cgcctcggaa cgcgtcctga
    24421 aggcgagaag gatcatcatt gagcttctcc tggcttcctg tccgcaatcc aaggtcattc
    24481 aggatatcgc gtcgcggcac aacgtgcgcc agcagcgctt ccggcaggag cacgaggact
    24541 gcatcctctg cggactgtgc gtgcggatgt gcaacgagca gatgatggcc ggagccatcg
    24601 gttttcgcgg acggggcaaa acgagaagcc tgggcacgcc tttcgacatg aaatccgacg
    24661 tctgccggct gtgcggcggc tgcatggagg tctgcccggc ctgtcaggcg cgctgcacgt
    24721 tcaatcagcc ggaaaaagcg atctgcggag gctgcgccaa ccttgcgccg ccgtgcattg
    24781 cgaaggatca gttccacgat atgatgtgtt acatgtcacc ctgcgtggcc tgtgaa
//
LOCUS       DAOPAF010000022        24013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2262_length_24013_cov_47.799274, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000022 DAOPAF010000000
VERSION     DAOPAF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24013
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2262_length_24013_cov_47.799274"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLQ77_03870"
     CDS             <1..236
                     /locus_tag="PLQ77_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09847.1"
                     /translation="EMVMTGKRCAAQECEEHHIITKACHIDQLMDEVMKFANLQNKRR
                     PVVELIKAEMNKDIVYAIDHEDPPIIASGRFYV"
     gene            complement(283..1590)
                     /gene="hisD"
                     /locus_tag="PLQ77_03875"
     CDS             complement(283..1590)
                     /gene="hisD"
                     /locus_tag="PLQ77_03875"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPL09848.1"
                     /translation="MKILRADAKAFHNFFAALRTRGGAFSPELLADVARIVQEVSARG
                     DKALFSYTRKFDGHDLTAATVEATDEEKKEALARVKPRDLEVIRLSAERIEKYHRRQI
                     AQGWSMKDEDGVELGQRILPLARAGIYAPGGKAFYPSTILMAAIPAHLAGVKEILLAS
                     PVKDGRLSPLIAAAAEAARVDRIFKIGGAQAVAALAYGTESVPRVDKIVGPGNAYVAA
                     AKKLVFGQVAIDMIAGPSEVAIIADQTADPVFVAADMLAQAEHDEKAAAVLFTPDPDL
                     AREVEGEIRRQMKTLSRKKIIQKSLSSFGAIIITADIDEAAALTNLFAPEHLELLVEN
                     PTNVLRQIRSAGSVFLGPYTPEALGDYIAGANHILPTEGTARFSSPLGVYDFYKRMSI
                     LSFPQAAFEKLAPQTRHFAEMEGLDAHANSVQVRCKSAKGSIA"
     gene            complement(1594..2841)
                     /gene="murA"
                     /locus_tag="PLQ77_03880"
     CDS             complement(1594..2841)
                     /gene="murA"
                     /locus_tag="PLQ77_03880"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPL09849.1"
                     /translation="MDKIVISGGRPLHGDVRISGAKNAALPVLTAALLTAEPCTFSNI
                     PDLVDIKTACKLLRNMGVEIEGAGTVKISAEKITDSVAPYDLVKTMRASILVLGPLVA
                     RTGKARVSLPGGCAIGARPVNLHIKALQDMGATVDLKEGYIEAKAGRLKGADIYFDLP
                     TVTGTENIMMAATLADGVTVLNNAAREPEIVNLAEVLQGMGANISGAGTDVITITGVS
                     ELSGTEAAVIPDRIEAGTFMIAAGITGGEINVLGCVPRHLEALINKLRDTGMRIRPTE
                     GGLRVAAGSKISSVDVKTLPHPGFPTDLQAQIMAYLCVASGLSVITETVFENRFMHVS
                     ELMRMGADITIQGGSAVVRGVPSLNGAQTMATDLRASASLILAALVAKGTTEISRVYH
                     IDRGYENIEKKFSALGADIKRVK"
     gene            complement(2842..3720)
                     /gene="prmC"
                     /locus_tag="PLQ77_03885"
     CDS             complement(2842..3720)
                     /gene="prmC"
                     /locus_tag="PLQ77_03885"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPL09850.1"
                     /translation="MRLAAKGKMTVREIIFEATQTLEKAGIPSARLDAEVLLAFLLRC
                     NRLAFIKNPDEAPSKKQLADFERLIARRANGEPVAYLTGRKAFWSFVLEVNPSVLIPR
                     PDTEVIVEEALSVAQREAWSNPRILDIGTGSGAIALALACEIPGAAITATDISSAALK
                     TAQKNAAALKLDQPVVFREGDLFEPVDGRFDLIVSNPPYIGAEDFESLPAGVKNFEPD
                     EALRAGQTGLEFYQKLIYQAHGYLAENGWLLLEIGATQEQEVRAIFEAHKDFYERVDV
                     REDYAGLPRVIKGRRR"
     gene            3926..4570
                     /locus_tag="PLQ77_03890"
     CDS             3926..4570
                     /locus_tag="PLQ77_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL09851.1"
                     /translation="MQAPDLKMSEKEKKILDAAIAVISEKGFSAASTSEIARQAGVAE
                     GTIFRYFKTKKDILRGILIHLGNVIAELLVVYGLKKILYQDGREDLRAGIKLVLKDRM
                     AMIDAVFPMLRIVITEAMYHEDIRQILYDKIISRSVEVFEEYHREMVEKGLMKREIDA
                     VVAGRGILGNLVLFFVYRNLFGPMIQLDDYDRELDQVIDAIMYGIAIPAVGDKK"
     gene            4599..5642
                     /locus_tag="PLQ77_03895"
     CDS             4599..5642
                     /locus_tag="PLQ77_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL09852.1"
                     /translation="MKMKMSGFFFLWPVLFLMFGCGDREAARPEAAPVFVIAKKIEKQ
                     AAARVIAVSGNIEGSKTARLGFMVAGKINYVAAREGEIIGKGQLLASLDPENYRIAKE
                     IADANVAQIQDEYSRLNLMHERGSISESDYSKISNGLKMARAQQRLHTKNLAETRLYS
                     PFRGILLKRGVEVGEIIGAGLPLFGVSDIRTVHVNASVPETDLRFIKKGVKAKIYVAS
                     IDAAFSGRVIEVGALAEPATRAFSVRMEVQNPGEILRPGMTAEIQIPSGQKEDVVKIP
                     GDAVLRDMDNTAYVFVADDVKHKAFRRKISLGKIDEDGIEVVAGLAPGELVVVGGQHK
                     LQNGSLIALKQVL"
     gene            5639..10150
                     /locus_tag="PLQ77_03900"
     CDS             5639..10150
                     /locus_tag="PLQ77_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPL09853.1"
                     /translation="MNFVKKSLKYPQVTLSILALTFLVGVYSLMAMPRREDAKIQIRV
                     GQVIALYPGANALQVEEQVTRKLEQHLFQYAEVRKEKTTSVTSDGMAVITVWLNNDVK
                     NFDTFWSKLNHQLNVLKALSLPQGVYGPIVNSEFGDTEALMIAIEMDRPQYAQLREYA
                     QKLEDTFRTMREVSKIKRIGEQKEQIVVYTDSAKLALYGVRLDQVMKILLSQNTIGPA
                     GGLKTSDVRVPLYTQGYYKTEAQIGDQIVGFAKTGEVVRLRDVARLQRQYEEPSSKIF
                     VNGKSSMLLAVQMHEGYNIVAFGKAVDRKLAEASQWLPAGVKLTTIVNQPKIVERNVS
                     HFLFEFVLAIAAVILVIILLLPFRVAAVAATAIPMTVAMTFALLHFFGIELHQVSLSA
                     LIVVLGMVVDDAVVVADNYVELLDNGVPPKTAAWKSATELVVPILTATLTIIAAFLPM
                     VLLSGMVRDFIIALPLTVSIALASSFFVAMILTPILCLSFIKKGLHDQERDLSSGRKK
                     MNLLNLMQSGYNRAIVWSMAHSRLTILVCLGAMGMAVVLYQILPQKFFPEAERNQLIV
                     DLWMPTGTKLEKTEQAIGGIQDLVQRDPDVVSLATFVGASIPRFYYNFTQGNPASNFA
                     QILVNTRLDEDAKRLRHELNLRADEAVPEGRLQARLLQQGVPSNASVEIRIIGDDIAT
                     LKEIGDRVMDILRQTRAVAFLRLDSREDYYGVAVRLKDGAERLGFTTESVAKSIYAGF
                     TGAPVSTLYEGNVAVDIVLRLDEKSRRHFGDVENLYLTSPVTGAGVPLRQIASLEPQW
                     LSGRINHLNGLRTLTVQCEPADGYLASQVLKMAGQRIADLSLPAGYKIEYGGEYENQK
                     ETFSEMLTALVVSLLLIFLVLLFQFRNLKETLIVMLTVPLSMFGAMLGLLMTGNPFGF
                     TAFVGLISLSGIVVRNAIILLDHADELRRGGASVATAALESGKRRLRPIFLTASAAAI
                     GVIPMIISGSPLWSPLASVIAVGIMLSMVTSLFLVPVLYACLMKPVDQDRKTPAGLQA
                     EGALSVGRKLMILVLASVCLLSFPSGAQEPPEALNLQKVIDLAAQNNRLLTIKQLQVQ
                     EKQQKVYEDRVKFFPSLIVGGAYQYNSNIGELSLKRGMLGQVAVGPISVPLPSADTTL
                     DLGHADTYTAGVLMYQPISQIPKIMAGVNVSKTDLEIAKTDQARATLQVKQTAEKLYF
                     GLLIQEKRREEAQIRLDLAQKKLYDVESAVLAGKTTPSSLTGLRANAADEEQNLLKIN
                     VQMDDYFDDLRRLTGLKSASFALAPVPVDDADPSAATNGVSGRQENQDLKIAALYQTK
                     AQHAVDAGHYSYLPSLGILGGYVYQHGAADFTGDIRYSFISAGFGLDPQWQKRDAFIG
                     VVLNWNLQDLVANTYVTRQRIFLKKQAEENLANTREQVHADTEKARRKLTQAAQLIGV
                     AGKVVAYRREDFKIQTDRFSAGLNLEADWLAAKAALAKSEADLLAARLSYRMAWTDLQ
                     ILTGRYGGE"
     gene            complement(10135..10839)
                     /locus_tag="PLQ77_03905"
     CDS             complement(10135..10839)
                     /locus_tag="PLQ77_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPL09854.1"
                     /translation="MADEIKTNILNICERIAAAAIRAGRDPAGIRLMAVSKTVEPERI
                     RQALDAGITLLGENYVQEAREKIPVVGRPAVWHMIGHLQTNKVKYVVNLFDWIHSVDR
                     LELARELDKRAGQRSRRLNVLIEVNVSGEASKNGAAPGQVLELVRQISILPNVSVRGL
                     MTMPPYSDDPENSRPCFIVLRQLRDEIVSAALPGISMDELSMGMTDDFEVAIEEGATI
                     IRVGRAIFGERRYSPP"
     gene            complement(10845..11444)
                     /locus_tag="PLQ77_03910"
     CDS             complement(10845..11444)
                     /locus_tag="PLQ77_03910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPL09855.1"
                     /translation="MIMVLSLSSPLILASASPRRRELLRSVGLTFKIRPADVDETGLP
                     DESPRAHVRRLSRDKAGAIAHQYPKALVLGADTIVVIDGRILGKPKNKKEAREMLQRL
                     SSRRHTVLTGFTIACVRAGMSRTKIVQSTVQFKKISPEETAWYVNGDEPYDKAGGYAA
                     QGKGASFIQAIRGSYTNVIGLPLCEVVEELNHLGVLHFR"
     gene            11899..12027
                     /locus_tag="PLQ77_03915"
     CDS             11899..12027
                     /locus_tag="PLQ77_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09856.1"
                     /translation="MIAFDDEKTNLDVIMKALRAGDFAVQDKPVYLKAFPAETQSN"
     gene            complement(12015..13091)
                     /locus_tag="PLQ77_03920"
     CDS             complement(12015..13091)
                     /locus_tag="PLQ77_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPL09857.1"
                     /translation="MHAVIMAGGRGTRFWPRSREKKPKHLLDIVSDRTIIQETVDRIK
                     PLIKPKNILIVTGKKHARELMRQLPEVPAKNIIIEPVGRNTAACIGLAALHIQKKVKD
                     DVMVVLPSDHGIADPARYRSVIAAAARAAEKKSALVTIGLTPASPHTGFGYMEGGASA
                     GNVAGEKLLRVKAFREKPNLEQARIFLQSGNFFWNSGMFIWNASTILKEIEKFLPDLH
                     SGLMTIKSSLGTSSEARTVSKIYRSLASVSIDYGVMEKAKDVFMIPAGFGWSDVGSWD
                     TLWEISQKDFKGNALAGGSQAMYENAENTLVYSPNKLVALIGIKDILVVETKDALLVC
                     QRGQSQDVKKIVDALEAEGKRQLL"
     gene            13225..14418
                     /locus_tag="PLQ77_03925"
     CDS             13225..14418
                     /locus_tag="PLQ77_03925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPL09858.1"
                     /translation="MIKINYPEIILKGGREAALLRGHPWVFSGAVAKIRGNPSDGDIV
                     LARDSRGQALALGFFNSRTDISFRVVSRDTAAAVDASFWNRRVLDALELRRRIIGEGT
                     SCYRLINAEGDGFPGLIVDVYNNTLVLSVTTAGMELQKHAILDALLFHLKPERIYEQS
                     AGRSRGLEGLSERRGFLHGPGQESAARVTENGYRFDVDFICGQKTGFFLDQRVNRERI
                     GSLARGRTVLNCFCYTGAFSVYAAGGGAKKVVSLDISKSACAAAAEHLRLNNCNPEDH
                     PVIEGDVFDYLRNLRECFDLIILDPPAFAKTKRDVTKASRGYKEINMQAIRHLSPGGM
                     LATFSCSNFIEEELFGKIVLGAARDAQADLQLLARLEAGPDHPLAAGHPEGRYLKGLL
                     LRKAA"
     gene            complement(14463..14858)
                     /locus_tag="PLQ77_03930"
     CDS             complement(14463..14858)
                     /locus_tag="PLQ77_03930"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPL09859.1"
                     /translation="MKRNLCALIVLVFLLSGCDAAMVMGGKVMGVSSGRFIYQDGYLS
                     TQYKAGVDQVWQACEKTVVELKGGKIEKDRKIAGGSIKAVISDEKVHILVEYVEKNVT
                     NVSVMCGIGGSNIASRMLQDRIAANIVKP"
     gene            complement(14922..15761)
                     /locus_tag="PLQ77_03935"
     CDS             complement(14922..15761)
                     /locus_tag="PLQ77_03935"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPL09860.1"
                     /translation="MSAQRNSILCPNCRKLISLDEPACPYCGLVRPGLHETAGKLRGI
                     LFAFEPVQTILYVNIAFFVLALLLDLRGIFTGGSPLAFLSPSQQSLLALGASGAIPVI
                     YYHNLWSIVSASFLHGGILHIVFNMMALYQLGPFVLREFGLHRFACIYILSGVAGFAV
                     SVLAGVQFTIGASASVCGLIGAIIYFGKSRGGSYGEAIFKQALGWVVGLIIFGFLFSG
                     INNWAHGGGLLTGLLLSFALGYSDQKPESAWSKILAYACVLITAAVLLWAAGSSLFYK
                     FLT"
     gene            15889..17046
                     /locus_tag="PLQ77_03940"
     CDS             15889..17046
                     /locus_tag="PLQ77_03940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPL09861.1"
                     /translation="MSIGERCRLNIQSVAFGGSGVGRAGGLVIFVPFTAAGDIADIEI
                     VERRKNFARGRLLGIVAPSPQRAEPLCRCFGRCGGCSYQHIAYHHQLNIKQAQVREAF
                     VKIGKLPDPPVEKVIASPRAYAYRGKAELHAAKSREGFRLGFMDITGGKLVDIERCEI
                     MHESINRQIIEARSRGRFESDGGDLIFWANPSSRAGDAIVRTVKDREFLIPLSGFFQT
                     NLYLTDAMVDEVRRLAGLEKRASILDACCGSGLFSIFLAQSAERLTGVEIHEKSVQCA
                     RENAERNGIRNAEFICADIGEHLNRMAERLDAVDLIVLDPPRAGLDSKALNAICGIKC
                     RDIIYVSCNPATQARDVKYLTANGYVLQSLQPLDMFSQTEHVETVGLLRRK"
     gene            17167..17559
                     /locus_tag="PLQ77_03945"
     CDS             17167..17559
                     /locus_tag="PLQ77_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09862.1"
                     /translation="MASKSREVRMEQRRILEKKLELRLQKLEALGAAKEKDPMVKNLR
                     SQIRETDIRIAAFDKNIAKIEILKQAKARKLAEKDAPREAPAAPEKEGKAKKKAAPAE
                     KESKKKAAPAEGEAPKKPKKKKEDAPAE"
     gene            17592..18020
                     /locus_tag="PLQ77_03950"
     CDS             17592..18020
                     /locus_tag="PLQ77_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09863.1"
                     /translation="MKRKMFFAFLVMLVLLPVQLPAADFDWMPNFNIQAQADPSGFHA
                     RLATRFNIGDAQVNAVLSNFPKPADAYVALRLGEMSGKPIDDVTGQYKANKGKGWGAL
                     AKSLGIKPGSKEFHALKRGDDLYGPKGKGKSKAKKDKKNK"
     gene            complement(18136..21453)
                     /locus_tag="PLQ77_03955"
     CDS             complement(18136..21453)
                     /locus_tag="PLQ77_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPL09864.1"
                     /translation="MMKRKSLKMIGAGFLWAAMFFATGAADCFAQQPKAPASPEKPEI
                     FVQLGHRSTDVKAAAFSSDGKYLVTGSGDKTVKLWHLSTGAEIRTFSGHEEEVTAVAI
                     SPDGSKILSGDKKGMIKLWDALSGNELRTMIVPWKEKEIWTLRFAPDGIRFSMLSGFS
                     LSFWDGGTGQALRKIDNIRGSYLADRGESYLAAKIGYKDDYALINPANAEEIKKFTYD
                     HQWFARASFSQDGKFVLFMTDDYSQKKYFFTLLDLTTGEKISSWQTAIEKDKNIYSIN
                     LLRDGKSALMSGGCGIELWDARKGKRLKVLTTSFTSMVNLSPDGRFFLSTGSRYAPTL
                     WDAASFSSVRSFRQRPLARCNSAQYRPDEKQVLINRTGAPPALLNLSTGGLEKTYSGY
                     SAGYFRAGGRSLLLIGEKGERLFQDLETHKVLKKFQSRYFDLPQDGKYLAELIGERDF
                     KVWETRADKEILNYREPSGSINSFTISSDNRHLVLCVDNRVKLIDIASGKETRSFTVP
                     DTANIHSAQISYDGRYLVAFYRDDVQQPKKLRAIVKIWNLETNQEIAKINNARNMLGF
                     KFSPDGRSLLVGDHTDRADSVWLIDLATGRIQKTLKGNDTIWATAFSADGRFLATGDA
                     DGSIMIWDVAAGRREMFVSGHKNGVRKIDFSSDNQQIVSTGDDGPARIWDIATGKEIA
                     QFFSFADGEWITITPEGYFNASAGGAKYLNVRLGNQIYSIDNFYETFFNPVYVASVLQ
                     GKKAEGLADIRTGIQSPPDVRILSPAPGAVLNTDTITIKVGAKDTGGGIDEIRLYHNG
                     KALGDDRRGVKLVGTGGETVRDFQVTLVDGTNIFRAVGFSSDRTESNPYEMTVSLSAP
                     SKDVSLYVFAVGVNQYRNPALNLNYAVPDAKGIAGFFRQKGQLFKKVNIIEIYDEKAT
                     KDGILAGLRELEKTNPQDAVLIYLAGHGENVKDVWYFIPHELAYPEREEYVQTKGISS
                     AELSSAMKNIRAQKILLLVDACKSGAVLVAFRGFEDRKALSQLSRSTGTHVVAASTKD
                     QFAAEVKDLGHGVFTYTLLEGLKGKAAGGSDTVTVRKLMGYIEEQLPEITKKYKQEAQ
                     FPVVDSRGMDFPLVIVK"
     gene            complement(21584..22489)
                     /gene="zupT"
                     /locus_tag="PLQ77_03960"
     CDS             complement(21584..22489)
                     /gene="zupT"
                     /locus_tag="PLQ77_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPL09865.1"
                     /translation="MDSVFIALGLTLIAGMATGIGSVIAFTAKRTDYRLLSVATGFSA
                     GVMLYVSFVEIFPESTAMLAARYGESWGSRLVTASFFGGMILMALIDNVIPAAENPHE
                     THSEEETAPLHDPKAALPDFPSAAFRDSTHPKPGVHDHGPFRAGLLRTGLLTALAITI
                     HNFPEGIATFLAAMQEPALGAAIAVAVALHNIPEGISVSVPIFYATGSRKKAFWWSFA
                     SGLAEPLGAVAALAVITLITGGGAWNPPVNLTGFLFGGIAGVMVYISLDELLPTSRAY
                     SKGHDSILGLMAGMMVMAISLLLMR"
     gene            complement(22529..23521)
                     /locus_tag="PLQ77_03965"
     CDS             complement(22529..23521)
                     /locus_tag="PLQ77_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPL09866.1"
                     /translation="MSERISSNFPAVKEFIKDNYRHFNAAVVVDAAQAYVEHLQQGGK
                     MMIAMGGAMSTAEIGITLAQMIRADKVHAISCTGANLEEDIFNLVAHKHYRRVPHYRD
                     LTPADEADLLKRKLNRVTDTCIPEEEAMRKVEKKLLGFWKQAEEENKRYFPYEFIFQL
                     LSSGVLKSDYEIDEKDSWLIAACQKNIPIFVPGWEDSTLGNVFVSSVRKGQIQNPAVV
                     KSGLETLNALIDWYLDASRDRGVGFFQIAGGISGDFAISVVPLIRQDMQMNCRLWSYF
                     CQISDSTTSYGSYSGAVPNEKITWEKLSVDTPRFIIESDATIVAPLIFAYVLAE"
BASE COUNT         5323 a         6672 c         6782 g         5236 t
ORIGIN      
        1 acgagatggt catgaccgga aagcgctgcg ccgcgcagga atgcgaggag caccatatca
       61 tcaccaaggc ctgccatatc gatcagttaa tggacgaggt catgaaattt gccaatcttc
      121 agaacaaaag acgcccggtc gttgaactga tcaaggcgga aatgaacaaa gacatcgttt
      181 acgcgatcga tcatgaggat ccgccgatca tcgcgtccgg tcgattctac gtttaatcca
      241 ataagcattt caatagcgta gggaacggtt taaaaccttt ccctacgcta ttgatccttt
      301 agcgctttta catcgaacct gcacggaatt ggcgtgcgcg tccagtcctt ccatttcagc
      361 gaagtgccgt gtctgcgggg caagcttttc gaaagccgcc tgcgggaaag acagaatgct
      421 catccgcttg tagaaatcat aaacgcccag gggcgaagag aaccgcgccg tgccttccgt
      481 gggcagaatg tggttggcgc cggcgatgta atcgccgagg gcttccggcg tatagggccc
      541 caggaacacg gagccggcgc tgcggatctg cctgagaaca ttcgtcggat tctccaccag
      601 aagctccagg tgctccggcg cgaagagatt ggtcagcgcc gccgcctcgt cgatatcagc
      661 cgtgatgatg atcgcgccga aggaagacag ggatttttga atgatttttt tccgggacag
      721 ggttttcatc tgccgtctga tttctccttc aacctcgcgc gccagatcgg gatcgggcgt
      781 gaacagcacg gccgccgcct tttcgtcatg ttcagcctgc gccagcatgt ccgccgccac
      841 gaaaacgggg tcggcggttt gatcggcgat gatcgccacc tcgctgggac cggcgatcat
      901 gtcgatggcc acctgtccga acaccagctt cttggccgcg gcgacatagg cgttgcccgg
      961 ccccacgatt ttatccacgc gcggaacgct ttcggtcccg tacgccaggg cggcgacggc
     1021 ctgcgcgccg ccgatcttga agatgcggtc cacccgggcg gcttcggccg ccgcggcaat
     1081 gagcggggaa agccggccgt cctttacggg gcttgccagc agaatttctt tgacgcccgc
     1141 gagatgcgcg gggatcgccg ccatcaggat cgtggaagga taaaaggcct ttccgcccgg
     1201 cgcgtaaatg cccgcccgcg caagcggcag gatccgctgg cccagttcga cgccgtcttc
     1261 gtccttcatc gaccatccct gggcgatctg ccggcggtga tacttttcaa ttcgttcggc
     1321 cgaaagccgg atgacctcca ggtcccgcgg ttttacgcgg gccagcgctt ctttcttttc
     1381 ctcatccgtt gcttcgacgg ttgccgcagt caggtcgtgg ccgtcgaatt tccgggtgta
     1441 ggaaaacagg gctttgtccc cccgcgcgga cacctcctga acgatgcggg ccacgtccgc
     1501 cagcagctcc ggcgaaaacg cgccgcccct tgtccgcagg gccgcaaaaa aattgtggaa
     1561 ggctttcgcg tcggctcgaa gaattttcat cttttatttt acccttttga tgtccgcgcc
     1621 cagcgcggaa aatttctttt cgatgttctc gtaaccgcgg tcgatgtgat agacccgcga
     1681 aatttccgtc gtgccttttg cgaccagcgc cgccagaatc agcgaggcgg aggcccgcag
     1741 gtccgtggcc atggtctgcg cgccgttgag agagggaacg ccgcgcacca cggcgctgcc
     1801 gccctgaatc gtgatgtccg cgcccatgcg catcagttcg ctcacgtgca tgaagcggtt
     1861 ttcaaaaacc gtctccgtga tgaccgacag gccgcttgcg acacacaggt aggccatgat
     1921 ctgcgcctgc agatccgtcg gaaagccggg atggggaagg gttttgacgt ccacgctgga
     1981 gattttgctt ccggcggcga cgcgaagccc tccttcagtg ggccggattc tcatgccggt
     2041 gtcccgcagt ttgttgatca gcgcttccag gtgccggggg acgcatccca gcacgttgat
     2101 ttccccgccg gtgatgccgg cggcgatcat gaacgtgccg gcttcgatgc ggtcggggat
     2161 gacggccgcc tcggttccgg aaagttcgga tacgccggtg atggtgatca cgtccgttcc
     2221 cgcgccgctg atgttggcgc ccatgccctg cagcacctcc gccaggttga cgatttccgg
     2281 ctcccgcgcg gcgttgttga gcacggtgac gccgtccgcc agggttgccg ccatcatgat
     2341 attttcagtg ccggtcaccg tgggcagatc aaaataaata tcggcgcctt tgagcctgcc
     2401 ggctttggcc tcgatatagc cttccttcag gtccacggtc gcgcccatgt cctggagcgc
     2461 cttgatatgg agattgacgg ggcgcgctcc gatggcgcag ccgccgggca gagaaacgcg
     2521 tgctttgccc gtgcgggcca ccagcggccc tagaaccagg atggacgcgc gcatcgtttt
     2581 gaccagatca tacggggcga cgctgtcggt gattttttcg gcgctgattt ttaccgtgcc
     2641 cgcgccttca atttccacgc ccatatttct taagagcttg caggccgttt tgatgtccac
     2701 caggtccgga atattggaaa acgtgcaggg ctctgcggtc agaagcgcgg cggtcagcac
     2761 cggcagggcg gcatttttcg cgccgctgat tcgaacgtct ccgtgaagcg gcctgccccc
     2821 ggaaatcaca attttatcca cttaccttct ccttcctttg atcacgcgcg gcaatcccgc
     2881 gtagtcttca cgcacatcga cccgttcgta aaaatcctta tgggcctcaa agatggcgcg
     2941 aacttcctgc tcttgtgtag cgccgatttc aagcaaaagc cagccatttt ccgcgaggta
     3001 tccgtgtgcc tgatatatca acttttggta aaattccaga cctgtttggc ccgcccggag
     3061 cgcttcatcc ggctcgaagt ttttcacccc ggcgggcagg gactcgaaat cctccgctcc
     3121 gatgtaggga ggattggaaa caatcaggtc aaaccggccg tcaaccggct cgaaaagatc
     3181 gccctcacga aacacgaccg gctgatccag tttcagggcg gcggcatttt tttgcgccgt
     3241 tttgagggcc gccgacgaga tgtccgtggc ggtaatggcc gcgccgggga tttcgcaggc
     3301 cagcgccagc gcgattgcgc cgcttcccgt gccgatgtcc agaatgcgcg gattgctcca
     3361 ggcttcccgc tgcgcaacgg aaagcgcctc ttcgacaatt acttccgtat cggggcgggg
     3421 aatcagcacg gaaggattta cttccaggac aaaggaccag aaagcttttc ggcccgtaag
     3481 gtaagccaca ggctctccgt tcgcccgtct ggcgatcagc cgctcaaagt cggccaattg
     3541 ttttttgctt ggcgcctcgt ccggattttt gatgaacgcc aggcggttgc accgcaaaag
     3601 aaaagccagc aaaacttcag cgtcaaggcg agcggaagga atcccggctt tctccagcgt
     3661 ttgcgtcgct tcaaaaatga tttcccggac cgtcattttt ccctttgcgg cgaggcgcat
     3721 tacacggccg ccccgatgat ttttcaatct atatcttatt tggaccgaat atccaaacgg
     3781 ccatttttta tggaacggaa cttcgctcaa aaaaatattg acaaaaacac aaaaatgagt
     3841 gattactcac tcaacataac ggcaacggag aaaagttttt gcaagtatta gtaattactg
     3901 agcttaatga aaaaaggtat tcatcatgca ggcgcctgat ttgaaaatgt cggaaaaaga
     3961 aaaaaaaatt cttgatgcgg cgattgccgt catttcagaa aaaggcttca gcgcggccag
     4021 cacgagcgaa atcgcgcgcc aggccggagt cgcggaagga accattttcc gctactttaa
     4081 aaccaaaaag gacattctgc gcggcattct gattcacctg ggcaatgtca tcgcggagct
     4141 gctggtggtg tacggcctca aaaaaattct ttatcaggac ggcagggaag accttcgcgc
     4201 cggcatcaag ctggttttga aagaccgcat ggccatgatc gatgccgttt ttcccatgct
     4261 gcgcatcgtg atcacggagg cgatgtatca tgaagacatc cggcagatcc tctacgacaa
     4321 aatcatttcc cggtccgtcg aggtatttga ggaatatcac cgggagatgg ttgaaaaagg
     4381 gctgatgaaa agggaaattg acgcggtcgt cgcgggccgc ggcattctgg gcaatctggt
     4441 tctttttttc gtttaccgca atctgttcgg ccccatgatc cagctcgacg attacgaccg
     4501 ggagctggac caggtcattg acgcgatcat gtacggaatc gcaataccgg cggtcggcga
     4561 caaaaaatag aggaagcggt tttgatcgag agggtcttat gaaaatgaaa atgtcgggat
     4621 ttttcttttt gtggcctgtt ctttttctga tgttcggctg cggcgaccgg gaggcggcca
     4681 ggccggaggc cgcccccgtt ttcgtgatcg ccaaaaagat cgaaaagcag gcggcggcca
     4741 gggtcattgc cgtcagcggc aacattgaag ggtcaaaaac cgcaaggctg gggttcatgg
     4801 tggccgggaa gatcaactat gtcgcggcca gagaagggga gatcatcggc aaggggcagc
     4861 tgctggcgag cctcgatcct gaaaattacc ggattgccaa ggaaattgcc gatgccaatg
     4921 tggcccagat ccaggatgaa tacagccggc tgaacctcat gcacgagcgc ggcagcatct
     4981 ccgaaagcga ctattccaaa atctccaacg ggctgaaaat ggccagagcc cagcagaggc
     5041 tgcacacgaa aaatctggcc gagaccagat tgtattcgcc cttccgcggc attttgctaa
     5101 agcgcggcgt ggaagtggga gagatcatcg gcgccggtct gccgctcttc ggcgtgtccg
     5161 atattcgcac cgtccatgta aacgcctctg tgccggaaac cgatctgcgc ttcatcaaaa
     5221 agggcgtgaa agccaaaatt tacgtcgcgt cgatcgatgc ggcgttttcg ggccgggtga
     5281 ttgaagtcgg ggccctcgca gaaccggcca cgcgggcatt ttccgtccgg atggaggtgc
     5341 agaatcccgg tgaaattctc cggccgggca tgacggctga gattcaaatc ccgtccggcc
     5401 aaaaagaaga tgttgtcaag attcccggag acgcggtttt gcgcgacatg gacaacacgg
     5461 cctatgtttt tgttgcggac gacgtcaagc acaaggcctt ccggcgcaaa atatcgctgg
     5521 gaaaaatcga tgaagacggc atcgaggtgg tcgcggggct tgcgccaggc gagcttgtcg
     5581 tggtcggcgg ccaacataag ctgcagaacg gatctttgat cgccctcaag caggtcttat
     5641 gaattttgta aaaaaatcgc tcaaatatcc gcaagtcacc ctgtccattc tggccctgac
     5701 gtttctggtc ggcgtgtatt ccctgatggc aatgcctcgc cgcgaggacg ccaagatcca
     5761 gatccgcgtg ggccaggtga tcgctctgta tcccggcgcc aacgccctgc aggtggaaga
     5821 gcaggtcacc cggaagctgg agcagcatct gttccagtac gcggaggtgc gaaaggagaa
     5881 aacgacctcc gtgacctcgg acggcatggc agtcatcacc gtctggctga acaatgacgt
     5941 caaaaatttt gacaccttct ggagcaagct gaaccatcaa ctgaacgtcc tgaaagcctt
     6001 gagcctgccg cagggggtgt atggtcccat cgtgaactcg gagttcggcg acacggaagc
     6061 gctgatgatc gccattgaaa tggaccggcc ccaatacgcc cagcttcggg aatacgcgca
     6121 aaaactggaa gacacctttc ggacgatgag agaggtgtcg aaaatcaagc gcatcggcga
     6181 gcaaaaggag cagattgtcg tttacaccga cagcgcgaag ctggcgctct acggggtccg
     6241 gctggaccag gtgatgaaga tcctgctctc gcaaaacacg atcggtccgg ccggcggcct
     6301 gaaaacgtcc gatgtccgcg tcccgcttta cacgcaggga tactacaaaa cggaagccca
     6361 aatcggagat cagatcgtcg gcttcgcgaa aaccggcgaa gtcgtccgcc ttcgggatgt
     6421 ggcccgcctt cagagacagt acgaggaacc gtcgtccaaa atcttcgtca acggcaaaag
     6481 cagcatgctg cttgccgttc aaatgcacga aggatacaac atcgtcgcct tcggaaaagc
     6541 ggtggaccgg aagctcgcgg aagcgtccca atggctgccg gccggggtca aactgaccac
     6601 gatcgtcaac cagcccaaaa tcgtagagcg gaatgtcagc cacttcctgt ttgagttcgt
     6661 cctggccatt gccgcggtga tcctggtcat cattctcctg cttcctttcc gtgttgccgc
     6721 ggtcgccgcc acggcgattc ccatgacggt cgcgatgacg ttcgccctgc tgcatttttt
     6781 cggcattgaa ctgcaccagg tttctctttc ggctctgatc gtggtgctgg gcatggtggt
     6841 ggacgacgcg gttgttgtcg ccgacaatta cgtggagctc ttagacaacg gcgttccgcc
     6901 gaaaaccgcc gcctggaaaa gcgcgaccga gctggtggtg cccatcctga cggcgaccct
     6961 caccatcatc gccgcctttt tgccgatggt cctgctgtcg ggcatggtga gagatttcat
     7021 cattgctctg ccgctgaccg tctccattgc cctggcgtcc tccttttttg tcgccatgat
     7081 tttaacgccg attttgtgcc tgtcttttat caaaaaaggt cttcacgatc aggagcgcga
     7141 tttatcttcg ggcaggaaga aaatgaatct gctcaacttg atgcagtccg gctacaaccg
     7201 cgccatcgtc tggagcatgg cgcattcacg gctgaccatc ctggtgtgcc tcggggccat
     7261 gggaatggcg gttgtcctgt atcagatcct cccgcagaaa ttttttccgg aagccgaacg
     7321 caaccagctt atcgtggatt tgtggatgcc caccggaaca aagctggaaa aaacggagca
     7381 ggcgatcggc ggaattcagg atttggttca acgcgacccc gacgtggttt ccctcgcgac
     7441 gtttgtcggc gcaagcattc cccgctttta ctacaatttc acgcagggaa accccgcttc
     7501 caatttcgcg cagattctcg tcaatacgcg cctcgatgaa gacgcaaagc ggctgcgcca
     7561 tgagttgaac ctgcgggccg acgaggcagt cccggaaggc aggctccagg cccgtctgct
     7621 ccagcagggg gttccctcga acgcctccgt ggaaatccgg atcatcggcg acgatatcgc
     7681 gacactgaag gagatcgggg accgggtcat ggatattctc cggcagacgc gggccgtcgc
     7741 ctttctccgc ctggacagcc gggaggatta ttacggcgtc gccgtccggc tcaaggacgg
     7801 cgccgaacgt ctgggattta ccaccgaaag tgtggccaaa tccatctatg ccggatttac
     7861 cggcgctccc gtatccacgc tctacgaagg gaatgtggcc gtggacatcg tgcttcgtct
     7921 ggatgaaaaa agccggaggc atttcggcga cgtggaaaac ctttacctga cgtctcccgt
     7981 gaccggagcg ggtgtgccgc tgcgccagat cgcctccctg gagccgcagt ggctaagcgg
     8041 ccggatcaac catctcaacg gactgcgcac gctgaccgtg cagtgcgaac cggcggacgg
     8101 ctatctggcc tcgcaggtgc tgaaaatggc cggacagcga atcgcagacc tttccctgcc
     8161 ggcgggctac aaaatcgaat acgggggcga gtacgaaaac cagaaggaaa ccttctccga
     8221 aatgctgacg gcgctggttg tcagcctcct tttgattttc ctggtcctgc ttttccagtt
     8281 ccgcaatttg aaggaaacgc tgatcgtcat gctgaccgtt ccgctaagca tgttcggggc
     8341 catgctgggg ctgctgatga ccggcaaccc gttcggattt accgcgtttg tcggcctgat
     8401 cagcctctcc ggcatcgtgg tgagaaacgc gatcatcctg ctggaccacg ccgatgaact
     8461 gcgccgcggc ggcgcgtccg tcgcgacagc cgcgctcgag tccggaaaaa ggcgactgcg
     8521 ccccatcttc ctgacggcca gcgcggcggc catcggcgtg attcccatga tcatttccgg
     8581 ctcaccgctg tggagcccgc tcgccagcgt gatcgcggtg ggaatcatgc tttccatggt
     8641 gacgtccctg tttttagtgc cggtgctgta cgcctgcctg atgaagccgg tcgatcagga
     8701 ccggaaaacg ccggccgggc tgcaagcaga aggtgctttg agcgtgggca ggaagttgat
     8761 gatccttgtc ctggcttccg tttgcctgct gtcttttcct tccggagcgc aggagcctcc
     8821 cgaagctttg aacctgcaaa aggtgatcga cctggccgcg caaaacaacc ggcttttaac
     8881 catcaagcag cttcaggtgc aggaaaaaca gcagaaggtg tacgaagacc gggttaaatt
     8941 ctttccgtcg ctgatcgtgg gcggcgccta tcagtacaac agcaacatcg gtgagctgtc
     9001 gctgaagagg ggaatgcttg gccaggtcgc cgtcggcccg atcagtgttc cgctgccgtc
     9061 ggccgacacg acgctggacc tgggccacgc ggacacgtac acggcgggag tgctgatgta
     9121 tcagcccatc tcgcagattc cgaaaatcat ggccggggtc aatgtgtcca agacggatct
     9181 ggaaatcgcc aaaaccgatc aggcaagagc gacgctgcag gtgaagcaga ccgccgaaaa
     9241 actctatttc ggcctgctga ttcaggagaa gcgcagggaa gaggcccaaa tccgactgga
     9301 tctggcccaa aaaaaactct acgacgtgga aagcgccgtg ctggccggca agacgactcc
     9361 ctccagcctg accggtctca gggccaatgc cgccgacgaa gagcaaaacc ttctcaagat
     9421 caacgtccag atggacgatt attttgacga tttaaggcgg ctgaccggtt taaaatccgc
     9481 ctcctttgca cttgcgcccg tgcccgtgga cgacgcggac ccgagcgccg caacaaacgg
     9541 tgtttccggg cggcaggaga accaggattt gaaaatcgcc gcgctgtatc agacgaaggc
     9601 gcagcacgcg gtggatgccg gacactacag ctacctgccg agtttaggca ttctgggcgg
     9661 ctacgtttac cagcacggcg cagccgattt taccggtgat atccggtatt cgtttatcag
     9721 cgccggcttc ggtctggatc cccagtggca aaagcgggat gcgtttatcg gcgtggtttt
     9781 gaactggaat ctccaggacc tggtcgccaa cacctacgtt acccgccagc gcatttttct
     9841 gaaaaagcag gccgaggaga atctggccaa taccagggaa caggtccatg ccgacacgga
     9901 aaaagcgagg cgcaaactga cgcaggccgc tcaattgatc ggcgttgccg ggaaagtcgt
     9961 tgcataccgc cgcgaagatt tcaaaattca aaccgaccgg ttttccgccg gactgaacct
    10021 ggaagcggac tggctggcgg caaaggccgc cctggcgaaa tccgaagcgg atctgctggc
    10081 cgcgcggctg agctaccgca tggcctggac cgaccttcaa atcctaaccg gacgctatgg
    10141 aggcgaatag cgtctttccc cgaaaatcgc gcggccgacg cggataatgg tggcgccttc
    10201 ttcaatggcc acttcaaaat catccgtcat gcccatggag agttcgtcca ttgaaatgcc
    10261 tggaagggcg gcggaaacga tttcatcgcg cagttgacga agcacaataa aacaagggcg
    10321 cgaattttcc ggatcatcgg aatagggcgg catcgtcatc aaacccctca cgcttacatt
    10381 cggtaagatg gaaatctgac ggaccagttc caaaacttgc ccgggcgctg cgccgttttt
    10441 cgatgcctcg ccgctgacat tgacctcgat gagcacattc aaccgtcggc tgcgctggcc
    10501 ggctctttta tccaactccc gcgccagttc caaacggtcc accgaatgaa tccaatcaaa
    10561 gaggttgacg acatacttga ccttgttggt ttgcaggtgg ccgatcatgt gccatacggc
    10621 aggccgtccg acgacgggga ttttctcgcg ggcctcctga acgtagtttt cacccaaaag
    10681 cgtgattccc gcatccaggg cctgccggat gcgttccggt tccacggttt tggagaccgc
    10741 catcagacga atgcccgccg ggtcgcggcc cgcgcggatt gcggccgctg cgattctttc
    10801 gcaaatgttc aaaatattgg tcttgatttc atcggccatg gtctttacct gaaatgaagg
    10861 acacccaaat ggttcaattc ttccacgact tcacaaagcg gcaacccgat cacattggta
    10921 taagagcccc ggatggcctg aatgaaggac gcgcccttgc cctgagccgc atagccgccg
    10981 gctttgtcat agggctcgtc gccattgacg taccatgccg tttcttcagg gctgattttt
    11041 ttaaactgca cggtggattg gacgattttt gttctggaca ttcccgcacg gacgcaggcg
    11101 atcgtaaaac cggtcaaaac ggtgtgtcgc cggctgctca gccgctgcag catttcacgg
    11161 gcctcttttt tgtttttggg ttttcccaga atccggccgt cgatgaccac aatggtgtcc
    11221 gcgcccagca ccagggcttt cgggtattga tgggcgatcg cgcctgcttt atctctggac
    11281 agccggcgaa cgtgagcgcg cgggctttca tccggaaggc cggtttcatc cacgtccgcc
    11341 ggacggattt tgaacgtcaa accgaccgaa cggagcagtt ctctccggcg tggtgaggcg
    11401 gaggccagga tcaggggaga cgacagggac agaaccataa tcatttcttt cttttttaaa
    11461 gtcgggcttt ttattccaga ataatcatgg cctgtcaatt cgaaattggc attgcgtttt
    11521 tcggaagcgt ggtttagtgg aagctcattg gtggatggtg aatcgtggat ggtgaatggt
    11581 ggactgtagg gaacggcttg tgacctttag gttatcgcga ccgttccatc gaaaaacagg
    11641 gacagcgccc atttactgta gggcgggcct tcaggcccgc agtgataaat catggatggt
    11701 gtgatgagaa aaatgaagag gctggttgcg atatttctgt ttctggtgct gtcggtcggt
    11761 gcggccggtg cggcggagag aatcgtgcag ttgaaggtgc ccggatgttt ttcgtgaggc
    11821 gccgaccgaa ggatagggac tatcctgaag aaaattgacg gggttcaaaa acatcagacg
    11881 caaagaccga acagcctggt gattgccttt gacgatgaaa agaccaacct ggacgtgatt
    11941 atgaaagccc tgcgggcagg cgattttgcc gtacaggaca agccggttta tctgaaagct
    12001 tttcccgccg aaactcaaag caactgacgc tttccctctg cctccagcgc gtccacgatt
    12061 ttcttgacgt cctgcgactg ccctctttgg cagaccagca gggcgtcttt cgtttcgacc
    12121 accaggatat cctttattcc gatgagcgcc accagcttgt tcgggctgta tacgagcgtg
    12181 ttttcggcgt tctcgtacat agcctgcgaa ccgcccgcga gcgcgttgcc tttgaagtcc
    12241 ttctgggaga tttcccacag tgtatcccaa cttcccacgt cgctccagcc gaagccggcc
    12301 ggaatcatga agacgtcttt ggctttttcc atgacgccgt agtcgatgga gacggacgcg
    12361 aggcttctgt agatttttga caccgttcgg gcttcggaag atgttcccaa agaagattta
    12421 atcgtcatca gccccgaatg cagatccggc aggaattttt caatttcttt caggatggtt
    12481 gaggcattcc agataaacat gccgctgttc cagaaaaaat tgccgctttg cagaaagatc
    12541 cgggcctgct ccaggttcgg cttttcgcga aacgctttga cccgcagcag cttttcgcct
    12601 gcaacatttc ctgccgaagc gccgccttcc atgtagccga acccggtgtg cgggctggcg
    12661 ggagtgaggc cgatggtgac cagggcgctt ttcttttcgg cggcccgggc cgctgcggcg
    12721 atgacgctgc ggtatcgtgc aggatcggcg atgccgtggt cggacggcag aacgaccatg
    12781 acatcgtctt tgactttttt ctgaatgtgg agagcggcga gcccgatgca ggcggccgtg
    12841 tttcgcccga ccggttcgat aatgatgttt ttcgcgggga cttccggcaa ttgtctcatc
    12901 agctcccgcg catgtttttt ccccgttacg atcagaatgt ttttgggttt gatgagcggc
    12961 ttgatccggt cgacggtttc ctgaatgatc gtccggtcgc tgacgatatc cagcagatgc
    13021 tttggcttct tttcccggct gcgcggccag aaccttgttc ctcttccccc ggccataatg
    13081 acggcatgca tgcggatgct cctgttattc atttattttt ttggcgatgc gggcgccgat
    13141 cctttgcgcg cggggacgcc ccttcggagg ctgtataata cgaaccggct tgcaaattca
    13201 agcccgaaac gctaatgaat cctcatgata aaaataaatt accctgaaat cattttgaaa
    13261 ggcggcagag aggcggcgct tttgcgcggc catccctggg ttttttcggg cgcggtcgcc
    13321 aaaatacgcg gaaacccgtc cgatggcgat attgtcctgg ccagggattc ccgcggacag
    13381 gcgctggccc tgggattttt caacagccgc acggatattt ccttccgtgt ggtcagccgc
    13441 gacacggccg ccgccgtcga tgcatccttc tggaaccgga gagttctgga cgcactggaa
    13501 ttgcgcaggc gaatcatcgg cgaggggacg agctgctatc gcctgatcaa tgccgaaggc
    13561 gacggctttc ccggcctgat tgtggatgtt tacaacaaca cgctggtttt gtccgtcacg
    13621 acggccggga tggagctgca gaagcatgcg attctggacg ccctgctttt ccacctgaaa
    13681 ccggaacgca tctacgaaca aagcgccggg cgctcgcgcg gactggaagg cctgtcggag
    13741 cgcagggggt ttctccacgg acctggtcag gaaagcgccg cgcgcgtgac ggaaaacggc
    13801 taccggtttg acgtggattt tatttgcggc cagaaaaccg gctttttcct cgaccagcgc
    13861 gtcaaccggg agagaatcgg ttccctggcc cggggcagga cggtgctgaa ctgcttttgc
    13921 tacaccggcg cgttttccgt ttacgccgcc gggggcgggg ccaaaaaagt agtttctctg
    13981 gacatttcca agtccgcctg cgcggcggcc gcggagcatc tgcgcctcaa caactgcaac
    14041 ccggaagacc acccggtcat cgagggggat gtctttgact atctgcgcaa tctgcgggaa
    14101 tgtttcgatc tgatcattct cgatccgccc gcgtttgcca agaccaaaag agatgtcacc
    14161 aaagcctccc gcggctacaa ggaaatcaat atgcaggcca tccggcatct gtcgccgggt
    14221 ggaatgctgg caactttttc ctgctccaat ttcatcgaag aagaattatt cggcaagatt
    14281 gttctgggcg ctgcgcgcga cgcgcaggcc gacctgcaac tgctggcccg cctggaagcg
    14341 gggccggatc atccgctggc cgcgggccat cccgaaggcc ggtatctgaa aggactgctg
    14401 ctgagaaaag cggcatgacg ggcgcaaacg gcgccgcttt gcctgcatgc cttcacgctt
    14461 cgtcagggct ttacgatatt ggcggcgatc ctgtcctgga gcatccgcga agcaatgttg
    14521 ctgccgccga tgccgcacat cacggacaca ttggtcacat tcttttcgac gtattcgacc
    14581 agaatgtgca ccttttcatc cgagatgacg gccttgatcg acccgccggc aattttgcgg
    14641 tccttttcga tcttgccgcc tttcagctcc actacggttt tttcacaagc ctgccatacc
    14701 tggtccacgc cggccttgta ctgcgtggag aggtagccgt cctgatagat gaatcttccg
    14761 gagctgacgc ccatgacttt tccacccatg accatggcgg cgtcgcaacc ggacagcaga
    14821 aaaaccaaaa cgatcagcgc gcaaagattt cttttcatga atttccctcc tggcgatcgg
    14881 ccgtgtccgc cggatgaagc agaggacatg gcgctttcag cttacgttaa gaatttgtaa
    14941 aacaacgacg accccgccgc ccagagcaga acggccgcgg tgatcagcac gcaggcgtat
    15001 gcaagaattt tgctccaggc gctttccggc ttctggtcgc tgtagcccag cgcgaaggac
    15061 aaaagcaggc ccgtaagaag gccgccgccg tgcgcccagt tgttgatgcc gctgaagaga
    15121 aatccgaaga tgatcagccc cacaacccag cccagcgcct gtttgaaaat ggcctctccg
    15181 taagagcctc cgcggctttt cccgaaataa atgatcgcgc cgatcagccc gcagacggaa
    15241 gcggacgcgc cgatggtgaa ctgcacgcct gccagcaccg aaacggcaaa accggcgacg
    15301 ccggacagaa tgtaaatgca ggcgaaacgg tggagcccga attcacgcag cacgaagggg
    15361 ccgagctggt agagtgccat catgttgaaa acgatgtgca gaatgccccc gtgcagaaac
    15421 gaggcggaaa caatgctcca gaggttgtgg taataaatga cgggaatggc gccgctggcc
    15481 ccgagcgcca gcaggctttg ctgggaaggc gaaagaaacg ccagcggact gccgcccgta
    15541 aaaatgcccc gcagatccag cagaagtgca aggacaaaga aagcgatgtt tacgtacagg
    15601 atcgtctgca ccggctcaaa cgcgaaaagg atgccccgca gttttccggc cgtttcgtgc
    15661 aggccgggac gcaccagtcc gcagtagggg caggccggtt catccagact gatcagcttg
    15721 cggcagttcg ggcagagtat ggagtttctt tgagcggaca tcgtgagttc cttctcgttt
    15781 ttgtggaggg aatccttaac aacaaaaaaa agagttgtaa agaaaaatgc gtccgcgcgg
    15841 ttgactcccg ccccgcaata taggatagtg ccgggcgtca ggaggatcat gagcatcgga
    15901 gaacgctgcc gtttgaacat tcaatctgtc gcctttggcg gatccggcgt cgggcgggcc
    15961 ggcggtctgg tgatttttgt gccgtttaca gcagcggggg acattgcgga cattgaaatc
    16021 gttgagcgca ggaaaaattt cgcccggggg cgtctgctgg gcatcgtagc gccctcgccg
    16081 cagagagcgg agccgctctg ccgttgcttc ggccggtgcg gcggctgctc ctatcagcac
    16141 atcgcttacc atcatcagtt gaacatcaag caggcccagg tgcgggaggc ttttgtaaaa
    16201 atcggcaaat tgccggaccc gccggttgaa aaagtcatcg cgtcgccgcg cgcctatgct
    16261 tacaggggaa aggccgaact gcacgctgcc aaatcacggg aaggctttcg cctgggattc
    16321 atggacatca caggcggaaa gctggtggac attgaacgct gcgagatcat gcatgagtcc
    16381 atcaatcggc agatcatcga ggcaagatcc cggggacgct ttgaatcgga tggcggcgat
    16441 ctgattttct gggccaatcc gtcaagccgc gcgggcgatg ctattgtgag aaccgtgaag
    16501 gaccgggaat ttctgatccc gctctcggga ttctttcaga ccaatttgta tctgaccgat
    16561 gccatggtgg acgaagtgcg ccgccttgcc ggcctggaaa aaagggcgtc cattctggat
    16621 gcctgctgcg ggtcggggct tttttccatc tttctggcgc aaagcgccga acggctgaca
    16681 ggcgttgaaa ttcatgaaaa gtccgttcag tgcgcgcggg aaaatgccga aagaaacgga
    16741 ataagaaacg cggagttcat ctgcgccgac atcggggagc acttaaaccg catggccgaa
    16801 aggctggatg cggttgatct cattgtgctg gatcccccgc gcgccggcct ggattcaaag
    16861 gcgctgaacg ccatatgcgg gataaaatgc cgggatatca tttatgtttc ctgcaatccg
    16921 gccacgcagg ctcgggacgt caaatacctg accgccaacg gatacgttct gcaaagtctt
    16981 caaccccttg atatgttttc gcaaaccgag catgttgaaa ccgtcggcct gctcaggagg
    17041 aaataaccgg gacgggaaga gacgaaagcc gggacggttc tttaaaaaat ggtggctttc
    17101 tggtgaaaat attgctactt tccccggcaa atcgagatta tttatctatt ttcaagggag
    17161 gataagatgg catcaaaaag cagggaagtc agaatggaac agcgccggat actggaaaag
    17221 aaactggagc ttcggcttca gaaattggaa gcgctcgggg ccgccaaaga aaaagaccct
    17281 atggtaaaaa atctgagatc gcaaattcgt gaaacggaca tccgcattgc cgcctttgat
    17341 aaaaacattg caaaaatcga aatattgaaa caggccaagg ccaggaaact ggcggaaaag
    17401 gatgcaccca gggaagcccc ggccgcgccg gaaaaagaag ggaaggcaaa gaagaaagcc
    17461 gccccggcgg agaaggaatc caaaaagaag gcggctccgg ccgaaggaga agccccgaag
    17521 aaaccaaaga aaaagaaaga agacgctccg gcggaataat gccgccaaaa caaaatctat
    17581 gggagggcaa catgaaaaga aaaatgtttt ttgcgttttt ggtgatgttg gtgctgcttc
    17641 ccgttcagct tccggcggcg gattttgact ggatgccgaa tttcaacatt caggcacagg
    17701 cggatccgtc cggattccac gcccggcttg ccacgcgctt taatatcggc gatgcgcagg
    17761 tgaacgccgt tctcagcaat tttcccaaac cggcggatgc ctacgttgcg ctgcgcctgg
    17821 gggaaatgtc gggaaagccg atcgacgacg tcaccgggca gtacaaggca aacaagggga
    17881 agggatgggg agcgctggcc aaaagcctgg gcatcaaacc cggctcaaag gaatttcatg
    17941 ccctcaaacg aggagatgat ctttacggtc cgaagggcaa ggggaaaagc aaggctaaga
    18001 aagacaaaaa aaataaatag gattccggac ggcagaagag aagggcacga taggtgtcgt
    18061 ctgcactgaa taaaggttgc ccgctttcgg ataaggcggg taaaccaaat tcattttcag
    18121 tgcttcggcg tgcttctatt tcacgatcac cagcgggaaa tccattccgc gggaatccac
    18181 caccgggaac tgcgcttcct gtttgtactt tttggttatt tccggaagct gctcttcaat
    18241 atagcccatc agtttgcgca ccgtgaccgt gtcgctgccg cccgcggcct ttcccttcaa
    18301 accttccagc aaagtatagg tgaacacgcc gtgccccagg tccttcacct cggcggcgaa
    18361 ctggtctttg gtggatgccg cgacgacatg cgttcccgta gatcttgaaa gctgtgagag
    18421 cgccttgcgg tcttcaaagc cccgaaaggc gaccagtacg gcgccggatt tgcaggcatc
    18481 gaccagcagc aggattttct gcgcccgaat atttttcatg gccgaggaca attccgcgga
    18541 cgatattcct ttggtctgaa catattcttc gcgctccgga taggccagct catgggggat
    18601 gaagtaccag acatccttca cgttttcgcc gtggccggcc agatagatca gcacggcgtc
    18661 ctgcggattg gttttctcca gttcgcgcag cccggcaaga atgccgtctt tcgtcgcttt
    18721 ttcatcgtaa atctcgatga tgttcacttt cttgaatagc tgacccttct gccggaagaa
    18781 gccggcaatg cctttggcgt ccggcacggc atagttgagg ttcagcgccg gattgcggta
    18841 ttggttgacg ccgacggcaa aaacataaag cgacacatcc ttggaaggcg cactgagcga
    18901 aacggtcatt tcatagggat tggattcggt gcggtcggag ctgaagccca cggcgcggaa
    18961 aatgttggtt ccgtccacca gcgtcacctg aaaatccctg acggtctcgc cgccggtgcc
    19021 cacgagcttc actccccgac gatcgtcgcc cagcgccttg ccgttgtggt agaggcggat
    19081 ttcatcgatg ccgccgcccg tgtctttggc tccgacttta atggtgatgg tgtcggtgtt
    19141 caggacggcg ccgggcgcgg gcgagaggat ccggacatcc ggcggcgatt gaatgcccgt
    19201 gcgaatatcg gcaagccctt ccgccttttt cccctgcagg acagaagcaa cgtaaaccgg
    19261 gttaaaaaat gtttcataaa aattatcgat cgaataaatc tggttcccca gacgcacatt
    19321 cagatacttg gcaccgccgg ccgaagcgtt gaaatatcct tccggtgtaa tggtaatcca
    19381 ttcgccgtcg gcaaaggaaa agaactgcgc aatttccttg ccggttgcga tatcccatat
    19441 acgggccggg ccgtcgtcac cggtcgacac aatctgctga ttgtcagatg aaaaatcaat
    19501 tttccggacg ccgtttttat gtccggagac aaacatttcc cgcctgcccg ccgccacatc
    19561 ccagatcatg atgcttccgt cggcatcgcc ggttgccagg aatcttccat ccgcggaaaa
    19621 ggctgttgcc cagattgtgt cgtttccttt caatgttttc tgaattcgac cggtcgccag
    19681 atcaataagc cagacgctgt cggcccgatc ggtatggtca ccgacaagca gcgatctgcc
    19741 gtcaggcgaa aatttaaaac ccagcatatt tctggcgtta ttgattttcg cgatctcctg
    19801 gtttgtctcc agattccata tcttcacaat cgcccgaagc tttttcggtt gctgaacatc
    19861 gtcacgataa aaagcgacaa gataacggcc gtcatacgat atttgagccg agtgaatatt
    19921 tgccgtgtcg ggaacagtaa aagagcgcgt ttctttcccg gaggcaatat cgatcagttt
    19981 gacgcggttg tccacgcaca gcacaagatg ccggttatcg ctggaaatcg tgaagctatt
    20041 gatggatccg gaaggctccc gatagtttaa aatttcttta tccgctctgg tctcccatac
    20101 tttgaagtct cgctcgccga tgagttccgc cagatatttt ccgtcttgcg gcagatcgaa
    20161 gtaccgggat tgaaattttt tcaaaacctt atgggtctcc agatcctgga agagacgctc
    20221 gcctttttcg ccgatcagca aaagagagcg gcccccggcg cggaaatatc ccgccgaata
    20281 tccggaatag gttttctcca ggccgcccgt tgacaaattc aaaagggccg gcggcgcgcc
    20341 ggttcgattg atcagcacct gtttttcatc cggacggtac tgcgcgctat tgcaccgcgc
    20401 cagcggccgc tggcggaagg atcgcacact agaaaatgat gccgcatccc aaagcgtcgg
    20461 cgcataacgc gaaccggtcg aaagaaaaaa tcttccgtcc ggagaaagat taaccatgga
    20521 ggtaaaactg gttgtcaata ccttcagcct tttacccttc cgggcatccc aaagctctat
    20581 gccgcaccca ccagacatta aggccgattt tccgtcacgg agaagattga ttgaatagat
    20641 gtttttgtct ttttcaatcg ccgtctgcca ggaagatatt ttttcacccg ttgtcaaatc
    20701 cagcagcgtg aaaaaatatt ttttctgact gtagtcatct gtcataaaaa gaacaaattt
    20761 accgtcctgc gaaaaggatg cccttgcaaa ccattgatga tcataagtaa atttcttaat
    20821 ctcctccgca ttggccggat tgatcaacgc ataatcatct ttgtagccga tctttgccgc
    20881 cagatacgat tcgccgcggt cggcaagata actgccgcgg atattatcga tttttctcag
    20941 cgcctgccct gttccgccgt cccagaagga caacgaaaat cccgacagca tactaaaacg
    21001 aattccgtca ggcgcaaagc gcagcgtcca gatttctttt tctttccagg gaacaatcat
    21061 ggtgcgcaac tcgttgccgg acaaggcgtc ccaaagtttg atcattccct ttttatcgcc
    21121 ggagagtatt ttcgagccgt ccggcgaaat cgccacggcc gtcacttctt cctcgtggcc
    21181 ggaaaacgtc cggatttcgg cgcccgtgga aagatgccag agcttaacgg tcttgtcgcc
    21241 gcttcccgtc acgagatatt ttccatcgga agaaaaagcg gcggctttca catcggtgct
    21301 gcgatgtccc aactgcacga aaatttccgg tttttcggga cttgccggtg ctttcggctg
    21361 ctgggcaaaa cagtcggccg ctccggtggc aaaaaacatt gctgcccaaa ggaatcccgc
    21421 gccgatcatt ttcaatgatt ttcttttcat catcgtctca ctcctgaatt gccggttgct
    21481 gtcaaaacag ccctttattg aaagccggtt tctattttag tcggctctgc gctattttgc
    21541 aaggctgctg ccgacgaagg tgccgtattc atgacgagcc gatctagcgc atcagaagca
    21601 gactgatcgc catcaccatc atgccggcca tcagccccag aatactgtca tgccccttgc
    21661 tgtaagcgcg gctggtgggc aggagttcat cgaggctgat atagaccatt accccggcaa
    21721 tgccgccgaa cagaaacccc gtcaggttca ccggagggtt ccacgcgccg cccccggtga
    21781 tcagcgtgat caccgccaga gcggccacgg cgcccagcgg ttcggcaaga ccgctggcga
    21841 aagaccacca aaaggccttt ttgcggctgc ctgttgcata aaatatgggg acggaaacgc
    21901 tgatgccttc gggaatgttg tgcagggcga cggccacggc aatggctgcg cccagcgccg
    21961 gctcctgcat cgcggccaga aaggtggcaa tgccttcggg aaaattgtga atggtaatgg
    22021 ccagcgccgt cagcagtccc gtgcgcaaaa gccccgcgcg gaacgggccg tgatcgtgaa
    22081 ctcccggctt aggatgagta gaatccctga atgcggcgga cggaaaatcc ggaagcgccg
    22141 cctttggatc gtgaagcggc gccgtctctt cttcggaatg cgtttcatgg gggttttcgg
    22201 cggcgggaat cacgttgtcg atgagcgcca tcaaaatcat gccgccaaaa aacgacgcgg
    22261 tcacaagccg gcttccccat gactcgccgt agcgtgcggc cagcattgcc gtgctttcgg
    22321 gaaagatttc gacaaaggag acatacagca ttacccctgc cgagaagccc gtcgccacgg
    22381 aaagcagacg gtaatctgtg cgcttcgctg taaaggcgat aacgctgccg atgccggtgg
    22441 ccatgccggc tatcagcgtc agccccaggg caatgaaaac agaatccatt ttccagtctc
    22501 tctcagttcc gcaatgatgg aaatcatttc attccgccag aacgtaggcg aaaatcagcg
    22561 gagcaacaat agtcgcgtcg ctttcgatga taaaacgggg cgtatccacc gacagctttt
    22621 cccaggtgat tttttcattg ggaacggcgc cgctgtacga tccgtacgag gtcgtactgt
    22681 cggagatctg gcagaagtag gaccacaacc ggcagttcat ctgcatgtcc tggcgaatca
    22741 gcggcacgac gctgatcgcg aagtctccgg atatgccgcc cgcgatttga aaaaagccga
    22801 cgccccggtc gcgcgaggcg tccagatacc agtcgatcag ggcattcaag gtttccaggc
    22861 ctgacttgac gacggcgggg ttttgaattt gtcctttccg gacactcgag acaaagacat
    22921 tgcccagcgt cgaatcctcc cagccgggaa caaaaatcgg aatgtttttt tggcaggcgg
    22981 caatgagcca ggaatccttt tcgtcgattt catagtcgct tttcaggacg ccgctggaaa
    23041 ggagctgaaa aataaattca taggggaaat atcttttgtt ttcctcttcc gcctgcttcc
    23101 aaaaccccag aagctttttt tcgacctttc tcatggcttc ttcttcggga atgcaggtat
    23161 cggtgacgcg gttcaatttt cttttgagca aatccgcttc atcggcgggc gtgagatcac
    23221 ggtagtgagg aacacggcga taatgcttat gggctaccag gttgaagatg tcctcttcca
    23281 gattggctcc cgtgcaggag atcgcgtgga ctttgtccgc ccggatcatt tgggcaaggg
    23341 tgatgccgat ttcggcggtg ctcatggcgc cgcccatggc gatcatcatc ttgccgccct
    23401 gctgaagatg ttccacatac gcctgggcgg catcgaccac cacggctgcg ttgaaatggc
    23461 gataattgtc tttgatgaat tccttgaccg ccggaaaatt ggaagaaatg cgttcgctca
    23521 tttgaatgct cctgatatgc gtttggcaga gccgcgtccg ccgcagacga ccggccgcgg
    23581 tttcagggaa gaaccgcgct tcgggaaagc agatttccaa aacagaatat gaaggggctg
    23641 ttttaccaaa gcgttctccc tttcgatata tcaatgttgc ggtgtgccgt tgctcatgaa
    23701 cgacgatgta aaacagatga agcccaaacg ttaaagaatt gatcaacaga ttaacaatta
    23761 tgggggctca tatcatggag cggcggaaat tgcaacccgt ctcgagcctt catccgtttg
    23821 catgagcgcc cgcaagccac agcaggaaaa ggttttaaac cgttccctgc ggtccatcat
    23881 gcctcacccc cattcttctt cacccgaaaa aactccgtgg caattcttgc aaggatcgga
    23941 tactatttgc ccggccgctg gctaattcaa tcaacgaaag gaggcaacga tgagacttaa
    24001 aacaaaagac gga
//
LOCUS       DAOPAF010000023        20815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2681_length_20815_cov_53.798507, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000023 DAOPAF010000000
VERSION     DAOPAF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20815
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_20815_cov_53.798507"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLQ77_03970"
     CDS             <1..653
                     /locus_tag="PLQ77_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPL09867.1"
                     /translation="YGSYLDDKDEIPSNAMMVAGLDTGVALLAGFALFPAIFALGFEP
                     GAGPGLAFITLPAVFSHMPLGAFFGVLFFALLAIAALTSAISLLEVVTAWLMDEKDWS
                     RRRAAAAMGLVIFIVGLPATLGYSVLKDFSFPGLNKDLLDTYDWISNSIFLPLGGFFM
                     AIFTGYVWDAKHAIAEVNKGHKRFRIGMWWALLIRYVVPVLIFFIMAVGIYDTFFR"
     gene            685..2277
                     /locus_tag="PLQ77_03975"
     CDS             685..2277
                     /locus_tag="PLQ77_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPL09868.1"
                     /translation="MSPRSGVSKNLFNAQDLRRIRSLGLRPSDVKKQLKGYRRGFSSL
                     TLVRPCVPGDGIQSLSPAERRRLIRLYDAEASGCKMVKFVPASGAASRMFALWYAASE
                     RGTFGSDELDRSFFRDLRQMPFYPFLKQSDSTRRMIRRRDVRSVLQSILFPSGLDFGG
                     LPKALIPFHAYRSGEVRTALEEHLREAADLITDGRGVCRLHFTVSAGHVKPVKTFLQS
                     CVPRLEESRGVRFRVDLSVQSPSTSIVAADGHHRPFRDEEGGLVFRPGGHGALLQNLQ
                     RIDADLIFVKNIDNIPPEFLQKKILPCKKLLGGLMLETRRQVYSCLKRLERKDLSLTD
                     LQAVSDFCRDALHSDLPKGFQKLKAEEQRQWLFRLLNRPLRVCGMVRNEGEPGGGPFW
                     VLEKDQSRTLQIVESAHVDFNVPQQAGIWLEASYFNPVDMVCCIKNYRGEKFILDDYV
                     NRDAYLISPKTEKGRELLAQELPGLWNGGMAFWNTIFVELPLAAFNPVKTIYDLLRPQ
                     HRTGRAVKSSIVPTRPEKRPAP"
     gene            complement(2228..3202)
                     /locus_tag="PLQ77_03980"
     CDS             complement(2228..3202)
                     /locus_tag="PLQ77_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HPL09869.1"
                     /translation="MSIKAIALFSGGLDSILAVRLVQQQGIDVAGLTFTTPFFNAVKA
                     RAAADRIRLPLLVQDITGEHLQMLKSPRYGYGKNMNPCIDCHTLMLKIAGGKMEQTGA
                     DFLVTGEVLGQRPMSQTRQSLRMVAKNSGYADDILRPLSAQLLDPIQAELEGKIDRSR
                     LLAISGRGRKEQMRLAEAFGISHYPPPAGGCLLTDPMFARRLRDLMLHGEDREVRDYE
                     LLKYGRHFRVPEAGKIIVGRNQSDNEALRELAAENDLVCLMADFPGPLVLAPGGSEAS
                     QGAAASLCVRYSDAPSDKEARVTCRRGGQSAILTARAVSPDVSERWMI"
     gene            complement(3280..5058)
                     /locus_tag="PLQ77_03985"
     CDS             complement(3280..5058)
                     /locus_tag="PLQ77_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPL09870.1"
                     /translation="MAHSFLWRGLLAILFAINFCLPALSAQEDSAQLTFRKTAVSKKS
                     VQYHTVRKDEAIYGIIRKMPGVTEKDIPHYLEIIKALNPRIKDWDKIYPGQKIALPSK
                     SIAGAGEEARGDATEDLSSAASGYPTYRVKKGDYLIRIVRRKLNIPTKTQQAMLRIKE
                     LNPSIKDANLLYVGQILRLPQARMQAGTGPAPSVRPGMDEAVPAPPPLSEGRPAQTGD
                     EQTAAAAMPEEQPAPAERTESAASLSPGARLEIIRHILTQMHGNLMTKGNYYLPISRT
                     AQLTIDCSAIPVVELDDQTTVFLEMKNRSSASLKKLIGGAWSNLHIVKMDAKDDVIVL
                     LKKIFAKSKDYEIIKAGKPIALGSEPDVEIPADWLINRKSKVRPSDTVQGLRLVYQNR
                     DILPRAVTRLARAHSLAVTEFSPVSGLVAPREELYSLPPLTVLPKSPAADLGFAMLSY
                     LKLPADRNVEIRVFNIEKDGFNLSIKADILVSQGGRKTVVFSRTPPQEFIHVLRKTGH
                     ELIFISDRDDPATNLEKILRGVNVPFASGYFLFSGADHDQPPYTIGFKGIKIKNDEDV
                     YAVPFDVDHDLLGLMKEAWSATLIRY"
     gene            complement(5218..6087)
                     /locus_tag="PLQ77_03990"
     CDS             complement(5218..6087)
                     /locus_tag="PLQ77_03990"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPL09871.1"
                     /translation="MGCKPKPEDIAGGQAILEGVMMRHGEKIAAAVRRPDREIVFQER
                     NYVPLTKRYKPLGWMFIRGTVSLFEMMIVGIQSLMFSAEVALSEEEKKPQGWEMYVSF
                     AISFAVAIFFFVVVPAFFFTRVKEYFDSLILLNILEGCVRLGIFLCFLSATLLMADMR
                     RVYMYHGAEHKTVFAWEDGQDLTVENVKKYSTRHPRCGTSFILFVMIVSILVFSLLGR
                     PDFLHRVIYKLLLMPVIAGISYEAIRFTGKYGRFKLVRLLSLPGLALQKITTREPDDD
                     QIEVAIAAMKKVI"
     gene            6239..6457
                     /locus_tag="PLQ77_03995"
     CDS             6239..6457
                     /locus_tag="PLQ77_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09872.1"
                     /translation="MAPMDPSTAFMKEYQERFEKKLKENEMALLEHWKSQLEKIESSR
                     PDSIASLQLQIRKVSEMMANRIKVLKKG"
     gene            6459..7037
                     /locus_tag="PLQ77_04000"
     CDS             6459..7037
                     /locus_tag="PLQ77_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL09873.1"
                     /translation="MESIADFLFEAGMLSKTPRSGYQFLGSGRESVAEHILRTVFIGY
                     ALCKLNPSLNELRVLKMCILHDLPEARTGDMNYVNKKYVEVNEAKAVRELTEGLFFGG
                     DIRQAIDEFNARETPEAKTARDADQIALILQLKEYGDLGNKYSDEWIRYALQRLATEE
                     GKKLADRIIQTDSSHWWFKDKSDWWVNGSNRA"
     gene            7151..7501
                     /locus_tag="PLQ77_04005"
     CDS             7151..7501
                     /locus_tag="PLQ77_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09874.1"
                     /translation="MTSPDEYRHVYEAKEKYILKAVAGVLEEKKIGRNVAIDYDNNRV
                     DSDFMISGDWRTKTNARVKRINWKECEVTLIVTTEKKTETGWEMRRLLQKEQYDTFFS
                     VIELKVYEEMSRMY"
     gene            7525..7920
                     /gene="mscL"
                     /locus_tag="PLQ77_04010"
     CDS             7525..7920
                     /gene="mscL"
                     /locus_tag="PLQ77_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPL09875.1"
                     /translation="MAPKPKVSALVEEFKNFALKGNVVDLAIGVIIGAAFGKIIDSLV
                     KHIIMPLISLVMPGEQGYLAWKWVVNGKELPYGLFIGEVVNFLIIALALYIFIVKFIG
                     MIVKNKEEAPPAPTKDQELLTEIRDLLKK"
     gene            complement(8119..8301)
                     /locus_tag="PLQ77_04015"
     CDS             complement(8119..8301)
                     /locus_tag="PLQ77_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09876.1"
                     /translation="MATIMPEAENVQKAIKWISGCLEDGEAQPVHMLIEKAVFKFDLS
                     PKDTEFLMGFFRNHKK"
     gene            8648..9226
                     /locus_tag="PLQ77_04020"
     CDS             8648..9226
                     /locus_tag="PLQ77_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPL09877.1"
                     /translation="MAKSIKGTQTEKNLLASFAGESQARNRYTFFASAAKKEGYEQIS
                     RFFLETAENEKEHAKVFFKYLEGGELEITASYPAGVIGDTKSNLEAAAAGENMEWTVL
                     YADAAKIAKEEGFPEVARSFEQIAKVEKFHEERYRKLAANIAKGEAFAKKEAVKWHCI
                     NCGYVYEGPEAPKECPACKHPQSYYEVLAENY"
     gene            9426..10058
                     /locus_tag="PLQ77_04025"
     CDS             9426..10058
                     /locus_tag="PLQ77_04025"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPL09878.1"
                     /translation="MSTKKQRRSENFKDRQKKKSTRIAYLAAIVGGSVLLLVIFFVLL
                     FNALFPPVDMEALNRKKISVRIYFADPQERFLVPEKRVIYKEEDPVLQAKEVVNALLD
                     GSKTKSKTGNVNTFPARVILKDIGLDKDGTAEVSFSSDLTKNHPGGSTAELMTIYSLT
                     NTLTEIVPQIRAVRILVDGKELSSIKGHISTAGPFLPDPDLYAPAKEEKS"
     gene            10066..12099
                     /gene="fusA"
                     /locus_tag="PLQ77_04030"
     CDS             10066..12099
                     /gene="fusA"
                     /locus_tag="PLQ77_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL09879.1"
                     /translation="MAPKSKISRTRNIGIVAHIDAGKTTVSERILFYTGKSYKMGEVH
                     DGEAIMDWMPQEQERGITITSAVTTCAWKNSEIHIIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVAGVEPQSETVWHQADKYGVPKIAFVNKMDRVGADFFHVVEMMRDRFGSLPV
                     PVQIPVGSEDSFRGVVDLINRKLLIWNESSQGTDFTVSEVPDHLAEDVSREREKMIET
                     LANCDDDLAEIYLAGQDATAAQIIAALRKATIALKTVPVLCGAALRNKGIQPILDAVI
                     HYLPSPEDIPPVQGINPKTKQQEVRHSSDKEPFSALAFKVQLDEGRKLTYLRVYSGQI
                     AAGGEIYNASKKKKEKIARLLQMHSNKRERIERASAGELVAVLGLKETTTGDTLCDEA
                     RPIILEQMEFYEPVISQAIEAKTPADQEKLAVALAKLTEEDPTLKVKYEDETAQTVIS
                     GMGELHLEIIVDRLNREFNARVNVGRPRVVYRETIQKRAQAEHLFEKELGDKKHFGQV
                     RLELSPRKRGIGNEIVNAIPEEMIPAEYHEAIEESIREAMSSGVLNSYPVIDVAVRLT
                     GGAFREGEASVQGYKIAAATAFRDACLAAEPVMLEPIMAVDIITPSEFMGDVIGDINA
                     RRGEIQAVNPKGPVSEIKAKVPLKALFGYSTDLRSATQGRAVFTIMFEEYNKA"
     gene            12203..13582
                     /locus_tag="PLQ77_04035"
     CDS             12203..13582
                     /locus_tag="PLQ77_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL09880.1"
                     /translation="MAVLKSAWERLRDYPIFESEREAYPQTFSEELNDQCGRILFVVC
                     LITTVAWLPYIYLDAGLHPEEPLLPVIRMGLSVLSLTLFILHVSKRFPRHNMLFLVLI
                     GAYLAVGCGLITALADADPAYMGGYIFILTVMALVPVDRRYAIIIIAASLLTFFVTGR
                     FENISFDRTSDRYSVLDLSSATVVAIAFIYLLHNTRLASWLKSRKIEQQSEEMRSDKE
                     KIDNLLLNILPPAVAEELKGQGYVKPVFYPSATIVFTDFVGFTKISETLTPDQLVREL
                     DSAFSRFDRVMDKYGLEKLKTIGDSYMYAGGVPLPNATHAIDAVLGALEIQSFVEQIN
                     LEKSQSGRPVFEIRIGINSGPLMAGVVGEKKFVYDVWGDSVNLASRMESSGGKGRINI
                     SESTHARIRDYFETEARGQVLAKNKGAVSMYFVNRLKKEFASDPEGRTPSESFWSIYK
                     KLQEENTAA"
     gene            complement(13599..14924)
                     /gene="trmFO"
                     /locus_tag="PLQ77_04040"
     CDS             complement(13599..14924)
                     /gene="trmFO"
                     /locus_tag="PLQ77_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPL09881.1"
                     /translation="MKSGRVMIIGGGLAGCEAAWQLLRRGHGVDLYEMKPRRFSPAHK
                     TEHLAELVCSNSLKSNQPDSAPGLLKEEMRRLGSLILSAADQTAVAAGSALAVDRNEF
                     SRRVEERLLGQEGFSLHRAEVADIPQDGLTILATGPLTSDALAQSISRMLGGNSYLYF
                     YDAIAPIVEADSIDMDKAYWASRYGKGTPDYLNCPLSREEYQRFREALLTGEKVAARP
                     FEDVRHFEGCLPIEILAARGEETLAFGPMKPVGLINPNTGKMPCAVVQLRRENSAGTL
                     FNLVGFQTKLTWPAQERIFRMIPGLERAQFARMGSIHRNTYIHSPSLLSPSLQFRKNP
                     DVFFAGQISGVEGYMESTAMGLLAGLNAAFILEGRSLPPPPRETAVGALAHYITSPES
                     AENFQPMNIHFGILPPVEAPGMKKKERHLFCVRRSLEALSDWMRDQGLA"
     gene            complement(14921..17257)
                     /gene="topA"
                     /locus_tag="PLQ77_04045"
     CDS             complement(14921..17257)
                     /gene="topA"
                     /locus_tag="PLQ77_04045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPL09882.1"
                     /translation="MANSLIVVESPTKVKTIKKFLGRDFDVVASMGHIKDLPKSSLGI
                     DLEKGFEPTYHVIESRKKIIDALKKAAKGAENIYLAPDPDREGEAIAWHIADVIGAKN
                     KKIYRVLFNDLTRNTVLEALQKPLKLDFDKYEAQQTRRILDRLVGYQISPILWDKVKR
                     GLSAGRVQSVAVRIICEREEEIGKFVPEEYWNLVAQFEASVPPSFEAKLIKIGGKKVK
                     VSNAEEAGKLAADIQAAEFTVEKLEKKEVKRSALPPFTTSKLQQEASRWLKFSAKKTM
                     SVAQKLYEGIELGPEGSVGLITYMRTDSYRIAEEPLAAVRGYIKENYSDAYVPAKPHF
                     YKNAQKAQDAHEAIRPSSMAHKPQDIKPYLTPDQFRLYQLIWNRFVASQMNPAVFDQT
                     TIDIAGAGCTFRAQGQVMKFPGFTIVYTEGKDEKEEENGNGKDKLLPDVAENEKIRLA
                     KLDTQQKFTQPPPRFSEASLVRELEENGIGRPSTYASILSTIQDREYVRLEKGKFFPT
                     ELGKIVTELLVHSFPTILDMAFTADMENKLDAIESGANQRVRTLQDFYSLFSGELSQA
                     KKGMRNVKQEETPTDLVCEKCDSPMVIKWGKNGRFLCCSNYPECKNTKNFTHDENGKV
                     KHMETQTTDVKCNKCGKNMVVKEGRFGQFLACSGYPECKNTMNATKNENGDVVAQEAP
                     QTDEVCDLCGKPMAVKRGRYGQFLGCTGYPECKNIKKLGRDGKVTQKEEVVLSDEVCD
                     LCGKPMAVKRGRYGQFLGCTGYPECKNIKKIPRKKSEE"
     gene            complement(17316..18491)
                     /gene="dprA"
                     /locus_tag="PLQ77_04050"
     CDS             complement(17316..18491)
                     /gene="dprA"
                     /locus_tag="PLQ77_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPL09883.1"
                     /translation="MNPEDAKYWLALKSLEGVGNTLVGPLLERFGSPASVFSAPVHEL
                     AGVRGMSRKIAEAILAFSDWDGILRQIDGLNQAGFRVVTFLDECYPKNLLNMYDRPAL
                     LYVAGVLNSDDISIAVVGSRHASTYGRYTTDRISRELALRGVTIVSGMARGIDSCAHR
                     GALAAKGRTIAVLGSGLDVIYPPENKKLFDAIVQQGAVISEYPPGTQPLPFHFPARNR
                     IISGISYGVLVVEAGEKSGSLITAKLAMEQGREVFAIPGPIDSASSRGANHLIKQGAK
                     LIDSVDDILEEILPQLERTAREPVSPDRKATQTGSEQPYPQKNGVPERAVLEKNVETL
                     LPTDRKILKAIADGKTHADDIIQETGIPPAEAMGALMRLELQGRVLQHPGKLFTLKK"
     gene            18659..20377
                     /locus_tag="PLQ77_04055"
     CDS             18659..20377
                     /locus_tag="PLQ77_04055"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09884.1"
                     /translation="MNKNIVFFICATLMFCSFSAFALTPSDPKGTVRPADGAAASKNL
                     HPEEAHPVLTIMPREVDLGFIQPGSTAFGEFKLSDITPGTVESAASCPDGWEALSGKA
                     LPLKVSHEPVSLRLELTVADTPEKARIDKTKDPAYAVRMKMEAAGRELICRKSLKPGH
                     HRKDIPLTLAGGARNIFVEFRIQTAQGIPSISLYPQRLDLGTQSPGKILSKKIELANR
                     SREKLHWSVSSHPEAGKSAPPPEPKQERYFSFRQEELPEPGKYVIPEHLKDAMELTGK
                     WTERNGYPVSRGGGALKYRFQGTGISIFLQSRTPEGNCVVYLDEAPVSLPDELSGESE
                     KEEILIAEGLADGPHTLNLVIRGGALELEGVKVFGREVQRGPKGWITVFPNSGTTLSE
                     IDYLNVKVDTTSLAPGIYGDDIVFQTNAGEETAEVYVSIVPDAGHKVLDVYLYSSDYD
                     YLYTSDPQTEARRLYQNRYVKEGIAFRLFAPQTPGTTAFHRWYNPAIKDHFYSHDRTG
                     GGKRLDGYIYEGNIGNIATSRMTNTRELYRWYNPSTGRHYYSTQPKVPPALKKYRFDG
                     IAGYVR"
     gene            20460..20536
                     /locus_tag="PLQ77_04060"
     tRNA            20460..20536
                     /locus_tag="PLQ77_04060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20494..20496,aa:Arg,seq:tct)
     gene            20548..20624
                     /locus_tag="PLQ77_04065"
     tRNA            20548..20624
                     /locus_tag="PLQ77_04065"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20582..20584,aa:His,seq:gtg)
     gene            20645..20721
                     /locus_tag="PLQ77_04070"
     tRNA            20645..20721
                     /locus_tag="PLQ77_04070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20679..20681,aa:Arg,seq:tcg)
BASE COUNT         4664 a         5688 c         5848 g         4615 t
ORIGIN      
        1 cctatggaag ctatctggac gacaaggatg aaattccgtc caacgccatg atggtggcgg
       61 ggcttgatac cggcgtagca ttgctggccg gatttgcact gtttcccgcc atttttgccc
      121 tggggttcga accgggcgcg gggccggggc tcgcctttat cacactgccg gccgtcttct
      181 cgcacatgcc cctgggcgcc tttttcgggg ttcttttctt tgctttgctg gccattgccg
      241 ccctgacgtc cgccatttcc ctgctggagg ttgtgaccgc ctggctgatg gatgaaaaag
      301 actggtccag aagaagagcc gccgccgcca tgggactggt catttttatc gtgggtcttc
      361 cggcgacgct gggatacagc gtcctgaaag atttctcttt tccgggattg aacaaggatc
      421 tgcttgacac ctacgactgg atttccaaca gcatctttct gcccctgggc ggctttttca
      481 tggccatttt caccggctat gtgtgggacg ccaaacatgc gattgccgag gtcaataaag
      541 gccacaaacg gttcaggatc ggtatgtggt gggctttgct gattcggtat gtcgttcccg
      601 tactgatttt cttcatcatg gcggtgggaa tctacgacac gtttttcagg taaacggtct
      661 gccgccgaac aggaggttta gcgtttgagc cctcgttccg gtgtgagcaa gaatcttttt
      721 aacgcgcagg atctgcgccg tatccgatcg cttggccttc ggccgtccga tgtgaaaaaa
      781 caactgaaag gctaccggcg cggattcagc tctttgacgc ttgtacgccc ctgtgttccg
      841 ggtgacggca ttcagtccct gagccccgcg gaacgcaggc gcctcattcg gctttatgac
      901 gccgaggcct ccggctgcaa aatggtaaaa tttgttccag cgtcgggcgc ggccagccgc
      961 atgtttgctc tgtggtatgc cgcatcggag cgcgggactt tcggcagtga tgagctggac
     1021 cggtcttttt ttcgcgattt gcgtcagatg cctttttacc ccttcttgaa acaaagcgac
     1081 tccacgcgcc ggatgattag aaggagagac gtcaggagcg ttctgcaaag cattcttttc
     1141 ccatccgggt tggatttcgg cgggcttccc aaagcgctca ttccctttca cgcctaccga
     1201 agcggagaag tccggacggc gctggaggaa cacctgcggg aagcggcgga cctcattacg
     1261 gacgggcgcg gagtctgccg gcttcatttc acggtttccg ccgggcatgt caaacccgtg
     1321 aaaacttttc tgcaatcctg cgttccccgg cttgaagaaa gccgcggcgt tcgttttcgc
     1381 gtggatttgt ccgtgcagtc cccctcgacc agcattgttg ccgccgacgg tcatcaccgg
     1441 cctttccggg atgaagaagg cggcctggtc ttccgtcccg gcggacacgg cgcccttttg
     1501 caaaatcttc agcggatcga cgccgatctt atttttgtca aaaacatcga caatattccg
     1561 ccggaattcc tgcagaagaa aattctgccc tgcaaaaaat tgctgggagg attgatgctg
     1621 gagacgcggc ggcaggttta ttcctgcctg aagcggctgg aacggaagga tctttccctg
     1681 acggatctcc aggccgtttc ggatttctgc cgcgatgcgc tgcatagcga cctgcccaaa
     1741 ggatttcaaa agctgaaggc ggaagaacaa agacagtggc tgtttcggct cctgaaccgg
     1801 ccgttgcgag tatgcggcat ggtcagaaac gaaggagagc cgggcggcgg gcccttctgg
     1861 gttctggaaa aagatcaatc ccggaccctg cagatcgtgg aatccgccca tgtcgatttc
     1921 aatgttcccc aacaggcggg catctggctt gaggcttcct acttcaatcc ggtcgatatg
     1981 gtctgctgta tcaaaaatta tcgcggggaa aaatttattc tggatgacta cgtcaaccgg
     2041 gacgcctatc tgatcagccc caagacggag aagggacgcg agcttctcgc gcaggagctg
     2101 cccggcctct ggaacggggg catggccttt tggaatacca tttttgtcga gcttccgctc
     2161 gcggctttca atcccgtgaa gaccatttac gatcttctgc gtccgcagca ccggaccggc
     2221 agggccgtta aatcatccat cgttccgaca cgtccggaga aacggcccgc gccgtgagga
     2281 tggcggattg gccgccgcgc cggcacgtga cgcgggcttc cttgtctgac ggggcgtcgg
     2341 aataacggac gcacagcgag gcggctgcgc cctgagacgc ttcactgccg ccgggggcca
     2401 gaacgagcgg accggggaag tcggccatca ggcagaccag gtcgttctcc gccgcaagct
     2461 cccgcagggc ttcattgtcg gactgattcc tgccgacgat gattttgccg gcttcgggaa
     2521 cgcggaagtg acgtccgtat ttcaagagtt catagtcgcg gacttcgcgg tcctctccgt
     2581 gcagcatcaa atcgcgcaga cgccttgcaa acatcgggtc cgtcagcagg cagcctccgg
     2641 ccggcggcgg gtaatgcgaa atcccgaagg cttccgccag gcgcatctgc tctttccgtc
     2701 cgcgtcctga aatagccagc aggcgcgacc ggtcgatttt tccttccagc tccgcctgga
     2761 tcggatccag caattgggcg gaaagaggcc gaaggatatc atcggcgtag ccggaatttt
     2821 ttgcaaccat acgcagcgat tgccgcgtct gggacatggg gcgctgtcca aggacctcgc
     2881 cggtcacgag aaaatccgcg cccgtctgct ccatctttcc gcccgcgatc ttgagcatca
     2941 gcgtgtggca gtcgatacag ggattcatgt ttttgccgta gccgtagcgc ggcgatttca
     3001 gcatttgcag atgttcgccc gtaatgtcct gcaccagcag gggcagacgg attcgatcgg
     3061 ccgccgctct ggctttgacg gcgttgaaga aaggcgtcgt gaaggtcaga ccggcaacat
     3121 caattccctg ctgctgaacc agccggacgg ccagaatgct gtcgagtccg ccggaaaaaa
     3181 gggcaatggc cttgatgctc atgaagtttc cttattgaaa aagcgcggcg ggatattttc
     3241 cgcagcgcta tcgtgcaatt tatcgacaga agcggcgcgc taataccgga tcagcgttgc
     3301 cgaccacgct tccttcatca atcccagcag atcatgatcg acgtcaaaag gaaccgcgta
     3361 aacgtcctcg tcgttcttga tcttgatgcc tttaaaaccg atggtgtagg gcggctgatc
     3421 atggtccgcg ccggaaaata aaaagtagcc ggacgcgaaa gggacgttca cgcctcgaag
     3481 aatcttttcc aggttcgtcg cgggatcatc ccggtcggaa atgaaaatga gttcgtggcc
     3541 cgttttccga aggacgtgaa tgaattcctg cggcggggtt ctggaaaaaa ccacggtttt
     3601 cctgccgccc tgtgaaacca ggatgtccgc cttgatcgac aggttgaagc cgtccttttc
     3661 aatattgaat acccggattt ccacgtttct atcggcgggc agcttcagat acgaaagcat
     3721 tgcgaagcca agatcggcgg ccggcgattt gggcaaaacc gtcaggggag ggagggaata
     3781 aagttcctcc cgcggagcga caagacccga gacgggggaa aactccgtga cggccaggga
     3841 atgagcccgg gcaaggcgcg tgacggcccg cggcagaata tcgcggtttt ggtaaaccag
     3901 tctcagcccc tggaccgtgt ctgatggccg aaccttgctt ttccggttga tcagccagtc
     3961 cgcgggaatt tccacatccg gctcggatcc cagggcgatg ggtttcccgg ctttgatgat
     4021 ttcgtagtct ttgctttttg caaatatttt tttcaaaagg acgatgacgt cgtccttcgc
     4081 gtccattttg acgatgtgca ggttgctcca cgcgccgccg atcagttttt ttaaagaagc
     4141 actggaccgg tttttcatct ccagaaagac ggtggtctga tcatcgagct ccactacggg
     4201 gatggcggaa cagtcaatcg tgagctgggc ggtcctggag atcggcagat agtaattgcc
     4261 tttggtcatc aggtttccat gcatttgcgt cagaatgtgc ctgatgatct ccagccttgc
     4321 gccgggggag agagaggcgg ccgattctgt tctttcggcc ggcgcgggct gttcttccgg
     4381 catcgccgcg gcggcagtct gctcatcacc cgtttgcgcc ggtcgaccct ctgacaaagg
     4441 cggcggggcc ggcaccgcct catccatgcc gggccggacg gaaggcgccg gtcctgttcc
     4501 ggcctgcatt cgggcctggg gaagtcgaag gatctggccc acatacagga gattggcgtc
     4561 cttgatggat ggatttaatt ctttgattcg aagcatggcc tgctgcgttt tggtcggaat
     4621 gttcaacttc cgccgaacaa tgcggatgag ataatcgccc tttttgacac gataagtcgg
     4681 gtatccggac gccgccgatg aaagatcttc cgttgcatcc ccccgggctt cttccccggc
     4741 gccggcgatg cttttcgaag gaagcgcgat cttttggccg gggtatattt tgtcccagtc
     4801 tttgatccga ggattcaacg ccttgatgat ctcgagataa tgcggaatgt ccttttcggt
     4861 gacgccgggc atcttgcgga tgatgccgta aatcgcttca tccttccgga ccgtgtgata
     4921 ctgaacggat tttttggaga cagccgtttt ccgaaaggtc aactgcgcgc tgtcttcctg
     4981 cgcggacaga gccggaagac agaaatttat cgcaaaaagg attgccagca gccctctcca
     5041 taaaaatgaa tgcgccattg cgggtcgctc ccgaaaaaaa ccagaatatt cttgcgtttt
     5101 caaatacttc aactttatga aaaagtcaat gttcatcggg attaaaacag caggaagaac
     5161 actctgtaaa ggtttgcgca aaacatcaca agacggcgca cggacgggaa agcgatctca
     5221 tatcactttt ttcatcgcgg cgatggcgac ttcaatctga tcatcatccg gttcgcgtgt
     5281 ggtgattttc tgcagggcaa gccccggcag gctcagcaga cggaccagtt tgaagcgccc
     5341 gtattttccg gtaaagcgta tggcttcata agaaataccc gcgattaccg gcatcagcag
     5401 aagcttgtaa atgacccggt gcaggaagtc cggacgtccc agcagggaaa agaccaggat
     5461 ggagacgatc atcacgaaaa gaataaagct ggtgccgcag cggggatggc gggtggagta
     5521 ttttttcacg ttttccaccg tcaggtcctg gccgtcttcc cacgcgaaaa cggttttatg
     5581 ctctgcgccg tgatacatgt aaacgcgccg catgtccgcc atcagaagcg tggcggacag
     5641 aaaacacaaa aaaattccca ggcggacgca gccctccaga atattgagaa gaatcaggct
     5701 gtcaaagtac tctttcaccc gggtaaagaa gaaagccggc accaccacaa agaaaaaaat
     5761 ggcgacggcg aagctgatgg cgaaggaaac gtacatttcc cagccctgcg gctttttctc
     5821 ttcctcggaa agagcgacct ccgcggaaaa catcaggctt tgaatgccga cgatcatcat
     5881 ttcgaaaagg ctgaccgttc cccggataaa catccatccc aggggcttgt atctcttggt
     5941 cagcggtacg taattacgct cctgaaaaac aatctcccgg tcgggacgcc ggacggcggc
     6001 ggcgattttt tcgccgtggc gcatcatgac gccttcgaga atggcctgtc cgccggcaat
     6061 gtcttcaggt tttggtttgc agcccatgac ttttgtttcc tgtcgcttat ttttgcagcg
     6121 tttccttata gcatttaacc ggggctgtca aatgatgcct gcagaaaagt ttgttgcatc
     6181 tttcaggatg aatgttatag agtcttgccg taatttcagg aaaacaggga ggatgcgcat
     6241 ggcgcccatg gatccgtcga ccgccttcat gaaggaatac caggagcggt tcgaaaagaa
     6301 actgaaggaa aatgaaatgg ccttgctgga acactggaaa tcccagcttg aaaaaataga
     6361 aagttcaaga ccggacagca tcgcttccct tcaactgcaa atccgaaagg tgtccgaaat
     6421 gatggccaac cggatcaaag tgctgaaaaa gggataagat ggaaagcatt gccgattttt
     6481 tatttgaagc ggggatgctc agcaagacgc cccgcagcgg ttatcagttt ctgggcagcg
     6541 gcagggagtc ggtggcggag catattctgc ggacggtttt tatcggctac gctctgtgca
     6601 agctcaatcc gtctttgaac gaactgcgcg tcttaaaaat gtgcattctt cacgatctgc
     6661 ccgaagcccg caccggcgac atgaactacg tcaacaagaa atacgtggaa gtgaacgagg
     6721 ccaaagccgt ccgggaactg acggaagggc tttttttcgg cggcgacatc cgtcaggcga
     6781 tcgacgagtt caacgcgagg gaaaccccgg aagcgaagac cgcccgggac gccgatcaga
     6841 tcgcgctgat tcttcagctg aaagaatacg gggatttggg caacaaatat tccgatgagt
     6901 ggatccggta cgcgctgcag aggcttgcca ccgaggaagg aaaaaagctg gccgaccgaa
     6961 tcattcaaac ggattcatcg cactggtggt tcaaggacaa aagcgactgg tgggtcaacg
     7021 gaagcaaccg ggcatagacc catgtcccaa acaggagaga tgaatgattc ataaatcacg
     7081 gtttttattt ttttcttttt tggcggttct ttgccttgcg tcgtgcgccg ggaaatttgg
     7141 atcggaggtc ttgaccagcc ctgatgaata caggcatgtt tacgaggcga aggagaaata
     7201 cattctcaaa gccgttgccg gcgtcctgga ggagaaaaaa atcggtcgga atgttgccat
     7261 tgattacgac aacaatcggg tcgattcgga cttcatgatt tcgggagatt ggcggaccaa
     7321 gacgaacgcc cgcgtgaaac ggatcaactg gaaggaatgc gaggtgacgc tgattgtgac
     7381 cacggagaaa aaaacggaaa cgggatggga aatgcgccgc ctgctgcaga aagaacaata
     7441 tgatactttt ttcagcgtga tcgaattgaa agtctatgag gaaatgtcca ggatgtattg
     7501 aagaaaagca cgaaaggagg aggcatggcg cccaaaccaa aagtatcggc attggtggaa
     7561 gagttcaaga atttcgcctt gaagggcaac gtggtggatc tcgccatcgg cgtgatcatc
     7621 ggagccgcgt tcggcaagat cattgattcc ctggtcaagc acatcatcat gccgctgatc
     7681 agcctggtca tgcccggcga gcaggggtat ctggcctgga agtgggtggt caacggcaag
     7741 gaacttccct acggactctt catcggggaa gtggtcaact ttctgatcat tgcgctggcc
     7801 ttgtatatct ttatcgtgaa atttatcggc atgatcgtga aaaacaagga agaggcgccg
     7861 ccggccccga ccaaagacca ggaactgctg acggagatcc gggatctgct gaaaaagtaa
     7921 cggttgtccg gaagtgtccc gaccgccgga ccggccgtaa aaataatctt cggcggtcgg
     7981 tttaagtctg ccctccttga aaaaacggag ggaaaatgcg gttccagcct tgagcctgaa
     8041 gacccgcggc cggttttcta aggttattgc gggatcagcg ccgggggcgt gacgcatgtc
     8101 ttgcgccccc ggcggcttct attttttgtg attgcggaag aaacccatca ggaattctgt
     8161 atccttgggg gacagatcaa acttaaaaac cgccttttcg atcagcatgt gaaccggctg
     8221 ggcttcaccg tcttccagac agccggagat ccatttaatc gccttctgaa cattttcagc
     8281 ttcgggcata atcgttgcca tattgtttca tcctccttcg taatgtttta agcagttttc
     8341 cgcggtcagg atttcatcga caaagcgcaa aaacttttca gctacatact acaatacgtc
     8401 agaaaaatta actaatattt ggctcgtttg acattaaaaa ttttatgatt aacttgacgg
     8461 cagtacggga gcaaatcatc cgcggcggag ctccagggca gtcgataacg aaggtgggga
     8521 gagaggcgga caagcgctgc ggccatcttg atgattcgcg gcagggtgca aaatagatcg
     8581 aataagggtt gataacacac cggaaaacat atacaaattc aaaaaaatga atgaaaggag
     8641 atgcagaatg gcgaaatcaa ttaaaggaac ccaaacggag aaaaacctgc tggcctcttt
     8701 tgccggcgag tcgcaggcca gaaaccgcta cacatttttt gcaagcgccg cgaagaaaga
     8761 aggatacgag cagatttcac gttttttcct ggaaacggcg gaaaatgaaa aggagcatgc
     8821 aaaagtattt ttcaagtacc tggaaggcgg cgagcttgaa atcaccgctt cttacccggc
     8881 cggcgtaatc ggcgacacca aaagcaatct ggaggccgcg gcggccggtg aaaacatgga
     8941 gtggaccgtg ctgtacgccg atgccgcgaa aatcgcaaaa gaggagggtt ttccggaagt
     9001 ggcccgctcc ttcgagcaga tcgccaaggt ggaaaaattc catgaagaac gctaccgcaa
     9061 actcgccgcc aacatcgcaa agggagaagc attcgcgaaa aaggaggcag taaagtggca
     9121 ctgcatcaac tgcggctacg tgtatgaagg gcccgaggcg ccgaaagaat gccccgcctg
     9181 caaacacccg cagtcctatt acgaggtgct ggccgaaaat tattaaaccg gccatctcca
     9241 ggacggatat cgcgatagcc cgccttttca gttcggcggg ctattttttt tgcgtttggc
     9301 ctagctttgc aattttcatc gggatgccgt tccggcggcc tatcgcacgg cctatcgaag
     9361 ggcttgacac tgaaggaaaa ataccctaga ttggcgcagt tttttcaaga aagaaggttg
     9421 tcgtcatgtc cacgaaaaag cagcgccggt ccgagaattt caaagatcgc caaaaaaaga
     9481 aatccacccg catcgcctat ctggccgcca tcgtcggcgg gagcgttttg ctgctggtga
     9541 tcttcttcgt ccttttattc aacgctttat ttccgccggt tgacatggag gccttaaacc
     9601 ggaaaaaaat atcggtccga atctattttg cagacccgca ggagcgtttt ctcgttcccg
     9661 aaaagcgggt gatttacaag gaagaagacc cggtgctcca ggcaaaggaa gtggtgaacg
     9721 cgcttctgga cggctccaaa accaaaagca aaaccggcaa cgtcaacacc tttccagccc
     9781 gcgtgatcct caaagacatc gggctggaca aagacggaac cgcagaggtc agcttcagct
     9841 ccgatttgac aaaaaaccat cctggcggct ccacggcgga gttgatgacc atctattctc
     9901 tcaccaacac gcttacggaa attgttcccc agatccgggc cgtccggatt ctggtggacg
     9961 gcaaagaact gtcatcgatc aaagggcata tttccacggc cggtcctttc cttccggatc
    10021 ccgatctcta cgcgcccgct aaagaggaga aaagctgaca ttcccatggc gccgaaatcc
    10081 aaaatatccc gtacgcgcaa catcggcatc gtcgcccaca tcgacgcggg caaaacgacc
    10141 gtcagcgagc ggatcctttt ttatacgggc aagtcctata aaatgggtga ggtgcatgac
    10201 ggtgaagcaa tcatggactg gatgccgcag gagcaggaga ggggcatcac cattacgtcc
    10261 gccgtgacca cctgcgcctg gaagaattcc gagattcaca tcattgacac gccggggcac
    10321 gtggatttta ccattgaagt ggagcggtcc ctgcgcgttc tggacggcgc ggtggtggtg
    10381 ttttgtgcgg tggccggcgt cgagccccaa tccgaaaccg tctggcatca ggcggacaaa
    10441 tacggcgttc ccaaaatcgc cttcgtcaac aaaatggacc gggtcggcgc ggattttttt
    10501 catgtcgtgg aaatgatgcg cgaccgcttc ggatctcttc ccgtccccgt gcagattccg
    10561 gtgggaagcg aagattcgtt tcggggcgtg gttgacctga tcaaccgcaa acttctgatt
    10621 tggaacgaat cgtcgcaggg gacggacttt accgtaagcg aggtcccgga ccacctggcc
    10681 gaagacgtat cacgcgagcg ggagaaaatg atcgaaaccc tggccaactg cgacgacgac
    10741 ctggcggaaa tttacctggc cggccaggac gcgacggcgg cgcagatcat cgcggcgctt
    10801 cgcaaggcga ccatcgccct gaaaaccgtg cccgtgctgt gcggagccgc cctgcgcaac
    10861 aaaggcattc agcccattct ggacgcggtc atccactatc tgccgtcgcc ggaggacatt
    10921 ccgccggtcc agggaatcaa cccgaaaacc aaacagcagg aggttcgcca cagctccgac
    10981 aaggagcctt tttcggccct ggcgttcaaa gttcagctgg acgaagggcg caagctgacg
    11041 tatctgcgcg tttattccgg acagatcgca gccggcggcg agatctacaa cgcttccaag
    11101 aagaagaagg aaaaaatcgc gcggcttctg cagatgcatt cgaacaagcg ggaaagaatc
    11161 gagcgcgcat cggcgggcga actcgttgcc gttctggggc tgaaggaaac gacaaccggc
    11221 gacacgctgt gcgatgaggc gcggccgatc attctggagc agatggagtt ttacgagccg
    11281 gtcatcagcc aggccatcga agccaaaact cccgcggacc aggaaaaact ggccgtcgcg
    11341 ctggccaaac tgaccgagga agatccgaca ttaaaagtca agtacgaaga cgaaaccgcg
    11401 caaaccgtca tttccggcat gggcgaactg cacctggaaa tcatcgtgga tcgcttaaac
    11461 cgcgaattca acgcccgcgt caatgtcgga cgtccccgcg tcgtgtaccg cgaaaccatt
    11521 caaaagcgcg cccaggccga gcatttgttt gaaaaggaac tgggggacaa aaagcatttc
    11581 ggccaggtcc gcctcgaact gtcgccgcga aagcgcggca tcgggaatga aatcgtgaat
    11641 gcgattcccg aagaaatgat ccccgccgag tatcacgagg cgatagaaga gagcatccgc
    11701 gaggcgatgt ccagcggcgt gctcaacagc tatccggtca tcgatgtcgc cgtccggctg
    11761 accggcggcg cgttccgcga gggagaagct tccgtccagg gatacaagat tgccgccgcg
    11821 acggcatttc gcgacgcctg ccttgccgcg gagcccgtca tgcttgaacc gatcatggca
    11881 gtggatatca tcacccccag tgaattcatg ggcgatgtca tcggcgacat caatgcccgc
    11941 cgcggagaaa tccaggcggt caatcccaag gggcccgtct ccgaaatcaa ggccaaggtg
    12001 ccgctcaagg ccctgttcgg ttattccacg gatctgcgct ccgccacaca gggccgggcc
    12061 gtgttcacca tcatgtttga agaatacaac aaagcatgag acgatgaggg aagacattcg
    12121 ccgatgaaat gaaaaacgtt tgtaattctg cgaacagaac gcccggccgg aagggccggg
    12181 gagaattctg aaggaggcaa acgtggcagt tctgaagagc gcgtgggagc gattgcgcga
    12241 ttaccccatt ttcgaaagtg aacgagaggc ctacccgcag actttttccg aagaattaaa
    12301 cgaccaatgc ggccggatat tatttgtcgt gtgcctcatc acgaccgtgg cctggctgcc
    12361 ctacatttac ctcgacgccg gcctgcatcc ggaagagccc ctgcttcccg tgatccgcat
    12421 ggggctgtcc gtcttgagcc tcacgctctt catcctgcac gtatcgaagc gcttcccccg
    12481 gcacaacatg ctgtttctgg ttctcatcgg cgcttatctg gccgtcggct gcgggctcat
    12541 caccgctctg gccgatgccg atcccgcgta catgggaggc tacattttca ttctgaccgt
    12601 gatggccctg gtgccggtgg accggcgcta tgccataatc atcatcgcgg cttccctgct
    12661 gacgtttttt gtcaccggcc gctttgaaaa tatttccttc gaccggacga gcgaccgcta
    12721 cagcgtcctg gatttgtcca gcgccacggt tgtcgccatt gcgttcatct acctgctgca
    12781 caacacaagg ctggccagtt ggctgaaatc cagaaagatt gaacagcaga gcgaggaaat
    12841 gcgatccgac aaggaaaaaa tcgacaacct gcttttgaat attctgccgc ccgccgtggc
    12901 cgaggagctg aaaggccagg gatacgtcaa gcccgtgttt tatccgtccg cgaccattgt
    12961 ctttacggat tttgtcggct ttaccaagat ttccgaaacg ctgacgcccg accagctggt
    13021 ccgtgagctg gattcggcct tttcccggtt tgaccgcgtg atggacaaat acggcctgga
    13081 aaaactcaag accatcggcg attcctacat gtatgccggc ggcgtgcctc tccccaacgc
    13141 gacgcatgcc atcgacgcgg ttctgggcgc tttggaaatt cagtcgtttg tcgagcagat
    13201 caatctggaa aagtcgcaga gcggacggcc ggtctttgag atccggatcg gcatcaacag
    13261 cggcccgctc atggcgggcg tcgtggggga aaagaagttt gtctatgacg tctggggcga
    13321 ttccgtcaac ctggccagcc gcatggaatc ttcaggcggg aagggaagaa tcaacatttc
    13381 cgaatccacc catgcaagga tcagggacta ttttgaaacc gaggcccgcg ggcaggtgct
    13441 ggcgaaaaac aaaggggcgg tatccatgta tttcgtcaac cggctcaaaa aagaatttgc
    13501 ctcggatccc gaaggccgaa cgcccagcga aagcttctgg tctatttaca aaaagcttca
    13561 ggaagaaaat acggcggcat aacgcctgtc atgatttgtc acgccaggcc ctgatcccgc
    13621 atccaatccg aaagcgcctc aagggagcgg cgcacgcaga aaagatgcct ttcctttttc
    13681 ttcattcccg gggcttccac cggcggaaga attccaaaat ggatgttcat gggctggaag
    13741 ttttccgccg attcgggaga cgtgatgtag tgcgccagcg cgccgacggc cgtctcccgc
    13801 ggaggcggcg gcagggatct tccctccagg ataaacgcgg cgttcaatcc cgccagaagc
    13861 cccatcgcgg tggattccat gtatccttcc actccgctga tctgtccggc aaagaaaaca
    13921 tcgggatttt ttctgaattg aagggaaggc gaaagaaggg aaggggagtg aatgtaggta
    13981 ttgcggtgaa tgctgcccat ccttgcgaat tgcgcgcgct ccaggccggg gatcatccgg
    14041 aaaattcgct cctgcgcggg ccaggtcagc ttggtttgaa aaccgaccag gttgaaaagc
    14101 gtgccggcgc tgttttcgcg ccttagctgg accacggcgc agggcatctt tccggtattc
    14161 ggattgatga gccccaccgg cttcatgggg ccgaacgcca gcgtttcctc tccgcgcgcc
    14221 gccagaattt cgatcggcag gcatccctcg aagtggcgga cgtcttcaaa cggacgcgcg
    14281 gcgacttttt ctcccgtcag cagcgcctcg cggaaccgct ggtactcctc ccgggaaagc
    14341 gggcaattca gataatccgg cgtacccttt ccgtagcggg aggcccagta ggctttatcc
    14401 atgtcgatgg aatcggcttc cacgatcggg gcgatggcgt cataaaaata cagataggaa
    14461 tttccgccga gcattcggga aatggactgt gcaagggcgt cggatgtgag aggtcccgtg
    14521 gcgaggattg tcaggccgtc ttgaggaatg tccgcaacct cggcgcgatg cagggaaaaa
    14581 ccttcctgcc cgagcagccg ctcctcgacc cggcgggaaa attcattccg gtccacggcc
    14641 agcgcgctgc cggcagccac cgccgtctga tcggcggcgg acaggatcag cgaaccgagc
    14701 ctgcgcatct cttccttgag caggccgggc gcactgtcgg gctgattgga tttgagagag
    14761 ttgctgcaga caagctcggc cagatgctcg gttttgtggg ccggggaaaa acgccgcggc
    14821 ttcatttcgt aaagatcgac tccgtgcccg cgccgcagga gctgccaggc cgcttcacag
    14881 cccgccagcc cccctccgat gatcatgacc cgcccggatt tcattcttcc gattttttcc
    14941 tggggatctt tttaatgttc ttgcattcgg gatagccggt gcagcccaga aactggccgt
    15001 agcgcccgcg cttgaccgcc atgggctttc cgcacagatc gcagacctcg tcggaaagaa
    15061 ccacttcctc cttttgggtg actttgccgt ccctgcccag ctttttgatg ttcttgcatt
    15121 cgggatagcc ggtgcagccc agaaactggc cgtagcggcc gcgcttgacc gccatgggct
    15181 ttccgcacag atcgcagacc tcgtccgtct gcggagcttc ctgggcgacc acgtcgccgt
    15241 tttcgttttt ggtcgcgttc atcgtgttct tgcactccgg atagccggag caggccagaa
    15301 actgcccgaa acgtccttcc ttgacgacca tgtttttacc gcacttattg catttaacgt
    15361 cggtggtctg ggtttccata tgtttgacct ttccgttttc atcgtgggtg aagttcttgg
    15421 tgtttttgca ttccggataa ttggaacagc acaggaaccg cccgtttttt ccccatttga
    15481 tcaccatggg agaatcgcat ttttcacaaa ccaggtcggt cggcgtttct tcctgcttga
    15541 cgttgcgcat gccctttttc gcctggctca gctccccgga aaaaagactg tagaaatcct
    15601 gcagcgtccg gacgcgctga ttggcgccgc tttcaatggc gtcaagcttg ttttccatgt
    15661 ccgcggtgaa agccatatcc agaatagttg gaaaactgtg caccagcagc tccgtgacaa
    15721 tttttcccag ctccgtcggg aagaattttc ccttctccag acgaacatat tcccggtcct
    15781 gaatggtgga aaggatcgat gcatacgtgc tggggcgtcc gatgccgttt tcctccagtt
    15841 cgcgcaccag cgacgcctcc gaaaaacgcg gcgggggctg agtgaacttc tgctgcgtgt
    15901 cgagcttggc gagcctgatt ttttcatttt ccgcgacgtc gggcagcagc ttgtcctttc
    15961 cgtttccgtt ttcctcttcc ttttcatcct tgccttccgt gtacacgatg gtaaagccgg
    16021 gaaacttcat gacctggccc tgcgcgcgga aggtgcagcc cgcgccggcg atgtcgatcg
    16081 tcgtctgatc gaaaacggca gggttcatct ggcttgccac aaagcggttc cagatcaact
    16141 gataaagccg gaactggtcg ggcgtcaggt agggcttgat gtcctgcggc ttgtgggcca
    16201 tcgaagaagg ccggatggct tcatgggcgt cctgcgcctt ctgtgcgttt ttatagaaat
    16261 ggggttttgc gggaacatat gcgtcggaat aattttcctt gatgtagccg cggacggccg
    16321 cgagcggctc ctcggcaatc cggtaggaat ccgtccgcat gtaggtgatc agacctacgg
    16381 acccttccgg ccccagttcg atgccctcgt aaagcttctg ggcgacgctc atcgttttct
    16441 tggcggaaaa cttgagccag cgcgaggctt cctgctgaag cttgctggtc gtaaagggcg
    16501 gcagggccga ccgcttcact tcctttttct ccagcttttc gacggtgaac tccgccgcct
    16561 gaatgtccgc cgccagtttg cccgcttctt cggcgttgga cactttcacc tttttcccgc
    16621 cgattttgat cagcttcgct tcaaaagagg gcggaacaga ggcttcaaac tgcgccacga
    16681 gattccagta ctcttccggc acgaacttgc cgatttcctc ttcccgctcg cagatgatgc
    16741 gcacggccac cgactgcacc cgtccggcgg agaggccccg tttgactttg tcccagagga
    16801 tggggctgat ctggtagccg acgaggcggt cgaggatgcg gcgcgtctgc tgggcctcgt
    16861 atttgtcgaa atccagcttc agaggcttct ggagagcctc cagcacggtg ttcctggtca
    16921 aatcgttgaa gaggacccgg tagattttct tgttctttgc gccgatgacg tcggcgatat
    16981 gccaggcgat ggcttctcct tcacggtccg ggtcgggcgc cagataaatg ttttcggcgc
    17041 ctttggccgc ttttttcagc gcatcgatga ttttctttct ggattcgatc acatggtagg
    17101 tgggttcgaa acccttttcc aggtcgatgc ccagcgaact tttgggcaga tctttaatat
    17161 ggcccatgga ggccaccaca tcaaaatccc gtcccaggaa tttcttgatg gtttttactt
    17221 ttgtggggga ttccacaaca attaatgagt ttgccataag cgctccttgc atgtgggcgc
    17281 accatataga tggtgaaaat gcatttgtaa agcttttatt ttttgagcgt gaacaatttt
    17341 cccgggtgct gcaaaacccg tccctgcagt tccaggcgca tcagggcgcc catcgcctcg
    17401 gcagggggaa tccccgtttc ctgaataata tcatcggcat gggttttccc gtcggcgatg
    17461 gccttcaaaa ttttccgatc ggtcggcaat agagtctcca cgtttttttc caaaacggcg
    17521 cgctccggca ccccgttttt ttgaggatag ggttgctccg atcctgtttg agtcgccttc
    17581 cggtccgggg aaaccggctc tctggccgtt ctttccaact gcggcagaat ctcttccaga
    17641 atatcatcca cgctgtcgat cagcttggcg ccctgcttga tcaggtggtt ggcgccgcgc
    17701 gaagacgccg agtcgatggg cccgggaatt gcgaaaacct cgcgcccctg ttccatggcc
    17761 agttttgccg taatgagcga gccgcttttt tccccggcct ccacgaccag aacgccgtag
    17821 gaaattccgc tgataatccg gttgcgcgcc gggaagtgaa acggcagggg ctgcgtcccc
    17881 ggcggatatt cggagatgac ggcgccctgc tgcacaatcg cgtcgaacag ctttttgttt
    17941 tccggagggt agattacatc caggccgctc cccagaacgg caatcgttct gccctttgcc
    18001 gccagcgcgc cgcggtgcgc acaggaatcg attccccgcg ccatgccgct gacaatggtg
    18061 acaccccgga gcgcaagttc ccggctgatt ctgtccgtgg tgtaccgtcc ataagtgctg
    18121 gcatgccggg acccgacaac ggcaatggaa atgtcgtcgc tgttcaaaac gcccgcaacg
    18181 taaagcaggg cagggcggtc atacatattc aaaagatttt taggatagca ttcgtccaga
    18241 aaagtcacca cccggaagcc cgcctgattc agaccgtcga tctggcgcag aatgccgtcc
    18301 cagtcgctaa acgcgagaat cgcttccgcg atctttctgc tcatgccgcg gactcccgcc
    18361 agttcatgaa ccggagccga aaagacggag gccggggagc cgaaacgctc cagcaggggg
    18421 ccaacgagcg tgttgccgac gccctccagg gatttcagcg ccagccagta ttttgcgtct
    18481 tcaggattca taggcaatga cgacagagtg ttggtatgca aatttatggt gccggaaaat
    18541 taggctgaac attacatctt ctcgatttac tctgtcaagt attttaaaat gatttaaaat
    18601 ggttgcgtaa aatgcttctt tacgctaaaa aagcgcaacc tattcaccag aggaatccat
    18661 gaataaaaat attgtttttt tcatttgcgc aactttgatg ttttgctcgt tttccgcttt
    18721 cgcgctgacg ccttcggacc cgaaagggac cgtcaggccg gccgacggag ctgccgcttc
    18781 aaagaacctt catccggagg aggcgcatcc cgtcctgacg attatgccca gggaagtgga
    18841 cctgggattc atccagccgg gctcaaccgc ttttggtgaa ttcaagctca gcgacatcac
    18901 gccgggaacc gtggaatccg cggcatcctg cccggacggc tgggaagctc tttccggaaa
    18961 agcgcttccg ctgaaagtct cccatgaacc ggtttctttg cggctggaat tgacggtcgc
    19021 cgacactcct gaaaaagcgc gcatcgataa aaccaaagac cccgcctatg cggtgaggat
    19081 gaaaatggag gccgccggca gggagttgat ctgccgcaaa agtctgaaac ccggacatca
    19141 ccggaaggac attccgctta ccctggccgg cggtgcaaga aatatttttg tggaattccg
    19201 gattcagacc gcacagggaa ttccgtccat cagcttgtat ccccagcgtt tggaccttgg
    19261 cactcagtcg cccgggaaaa ttctgtccaa aaagatagag ctggccaaca gaagcaggga
    19321 aaagcttcac tggtccgtct ccagccaccc ggaagccgga aagtccgcgc ctcccccaga
    19381 acccaaacag gaaaggtact tttctttccg ccaggaggaa ttgccggagc ccggaaaata
    19441 cgtcattccc gagcacctca aggacgccat ggagctcacc ggaaaatgga ccgaaagaaa
    19501 cggctacccc gtaagccggg gcggcggcgc gctgaaatac cgctttcagg gaaccggcat
    19561 cagcattttt ctgcagtctc gcacgccgga aggaaattgc gttgtttatc tggacgaagc
    19621 cccggtgagc ctgccggacg aactgtccgg ggagtcggaa aaagaggaaa ttctaatcgc
    19681 ggaaggcctg gccgacggcc cccacaccct taacctggtc atccgggggg gcgctctgga
    19741 actggaaggc gtgaaagtat tcggccggga agtacagcgg gggccgaagg gctggatcac
    19801 ggtttttccc aattccggca caacgctgag cgaaatcgat tatctcaacg tcaaggtgga
    19861 tactacctct cttgcgcccg gcatttacgg cgacgatatc gtctttcaaa ccaacgccgg
    19921 ggaggagacc gcggaggtgt atgtcagcat cgttcccgac gcgggtcata aagtcctgga
    19981 tgtctattta tattccagtg attatgacta tctctacaca tcggaccccc agacggaagc
    20041 ccggcgtctt taccagaacc gatacgtcaa ggaaggcatt gcctttcggc ttttcgcgcc
    20101 gcaaactccc ggcacgaccg cctttcaccg ctggtacaat cccgccataa aggaccattt
    20161 ttattcccac gaccggaccg gcggggggaa aaggctggat ggatatattt atgaagggaa
    20221 cattggcaac atcgccacgt cccgcatgac caatacccgc gaattgtacc gctggtacaa
    20281 tccgtccacc ggccggcact actattcgac ccagccgaaa gtgccgcccg ccctgaagaa
    20341 ataccgcttt gacggcatcg ccgggtatgt ccggtagaat aaaagattca ccctttttaa
    20401 ccttgacaaa aaaaagaaga ggggatatta ggcgttctct ttttggtgca ggattaaatg
    20461 cgcctgtagc tcagctggat agagcaacgg acttctaatc cgtgggccga gagttcgaat
    20521 ccctccaggc gcaccaaggc gataatggtg ggtgtagctc aattggtcag agtgccgggt
    20581 tgtggccccg gaggctgagg gttcaagtcc cttcactcac cccattttta ggattggaaa
    20641 ctacgcgccc gtagctcagc tggatagagt cgcagacttc gaatctgttg gtcgtaggtt
    20701 cgaatcctac cgggcgtacc aggatagaga agttcttttc ataacggtgt tttcttaccc
    20761 aaagggccct tagctcagct ggtagagcaa ctgactctta atcagtaggt tgtcg
//
LOCUS       DAOPAF010000024        20370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2757_length_20370_cov_44.545902, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000024 DAOPAF010000000
VERSION     DAOPAF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20370
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_20370_cov_44.545902"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLQ77_04075"
     CDS             <1..419
                     /locus_tag="PLQ77_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09885.1"
                     /translation="MIDIESVIPHREPIKIITEAIELQDDAGTAAAVVNERWPLFDGR
                     TVHSLVLVEAVAQTAALVEGYKRKKRGQDSIKGWLVGIKSAEFHRELLALQTRITVSI
                     KSLYSFDDYAVINGIVTAENETLLTAVLQVVRLNQD"
     gene            453..1187
                     /gene="fabG"
                     /locus_tag="PLQ77_04080"
     CDS             453..1187
                     /gene="fabG"
                     /locus_tag="PLQ77_04080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPL09886.1"
                     /translation="MEERKIALVTGASRGIGRATAVELARTGCHVIINYNASEAAAAE
                     TLAQVQAAGGSGEMRKFDVSDPAAVKEAMAAILQEHKRIDVLVNNAGVTADGLFMMMG
                     EEEWHRVIQTTLNGFFHVTKPVLREMVRKKRGSIVSVSSISSMVPNRGQANYAAAKAG
                     INAATSSLAKEVARFGIRVNAVAPGPIATDMLKSMPMDESIIKSVIPMARVGKPEEVA
                     RVIRFLCSEDASYITGQVISVNGGMF"
     gene            1187..1813
                     /locus_tag="PLQ77_04085"
     CDS             1187..1813
                     /locus_tag="PLQ77_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPL09887.1"
                     /translation="MRGQAGRRLLILLFLLLTAFPDAAGADDFDRLRRDASGISSLSA
                     DFVQKKSMKILSAPLVSKGKFYYAAPDSIRWEYVKPIRSIVISNQGSTKRYIASGGKM
                     IEDKTGGALAMKIVLDEVAGWMKGKFTANPSFAARVQEGPETQIVLTPTGKNTAGLIE
                     KIEITVSRKDAVVKTVRIVEGAAAETRIDFLHTVVNQTLPPGTFQDIP"
     gene            1810..2472
                     /locus_tag="PLQ77_04090"
     CDS             1810..2472
                     /locus_tag="PLQ77_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09888.1"
                     /translation="MNLRLLLILPLLLASCSSLPEIRPSAVVAGKVIACPSPFVARKT
                     LFIHAVEAAAAGETRAVMIGVTVADPASRTLSCALMSPEGMVLFEASRNPSGTQISRA
                     LPPFDEEEFARGLLDDIELIFFEPPEPVDRAGFSDTGRFVCRRRLQGGGWLDVGKDGD
                     GRVQIRRYSKGGTLRRSVALAAGANPYAAIELQASEWIGYSLSMTLVEPEAVLDEALS
                     GK"
     gene            2491..3963
                     /locus_tag="PLQ77_04095"
     CDS             2491..3963
                     /locus_tag="PLQ77_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPL09889.1"
                     /translation="MRKPAEGKNTVIIGSGVGGLSAGILLSLLGFRVTVVEKNAEPGG
                     LMRSYSRRGIDCPVGVHYVGALGPSEPLGKMFRVLGIDPDSLFSRMKNRAVIDRYLFD
                     DFVFDLPEGLDAYEKNLREACRADGAALDALMKGLRGTAGVMAASPFFFEGGSPFANM
                     DSFRPMGELLCELGASSKLRAVLAAPCQLVGVELKDCPVIIHQMVLAGYLFSSWRPKS
                     GRDLADAFAARFSQLGGRLRLSSAVRHIRTKQGRVAGVDLEEGDSLPADSVVAAVHPK
                     VLIGLLDPEALRESQRERIAGLEETGGVLAVQAGVDAEAHPAVDYNLYRLRAGGSGEI
                     ENGFFCQVLKGDGRNTNLLSIITGSHYRDWLPWEDTLTRRRGEAYEQKKQALAEALVA
                     ESSAVFGPLKNLQLLDVFTPLSLRDHVSCPEGSCYGVLRSSRHLLKIASLGNLPIGGL
                     YLAGQNAMAPGVMGCILGSLGVARKMAGNERFGKELERLL"
     gene            4026..4298
                     /locus_tag="PLQ77_04100"
     CDS             4026..4298
                     /locus_tag="PLQ77_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPL09890.1"
                     /translation="MTRQEIEKEIRAVLLREFEVENPEPDVNLREAYGFDSIDAIELL
                     LEIERFLGTELTQAEKKKAMDIRTLNQIIDYVEMLAHHRKNEAEKA"
     gene            4299..5540
                     /locus_tag="PLQ77_04105"
     CDS             4299..5540
                     /locus_tag="PLQ77_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPL09891.1"
                     /translation="MPQRRVVITAASAISPIGHGRKPIVESLVNGKSGVAALREDHLI
                     SRFIHSRVYGTVGYPIAYDFSRQYRKTMGPVSYYACQVAREVLEQAGLPPDFVTGGRL
                     GIAFGSIHGSPTVQRDIYRIFFEAKDKATFQGIGAVDYLKSMVHTTAVNISKMFGITG
                     RIISSGTACTTASQSIGFGYEAVKYGLQDAMICGGADEYDTITVAVFDNLLACSTAFN
                     DRPGETPRPFDRARDGLVVAEGAGAVMLEEYEFARKRGATILAEVIGFACNSNGGDLI
                     LPNLDGITKTLRLGLENAQINASDVDLVSAHATATKMGDIIEAQATGRVFGTQPRVVA
                     LKSYMGHTMAACGAVETVLTIYMMQEGFIAPTLHLEEIDERCAMIRHVQKLEEIPVKT
                     AAIQNFAFGGVNTCLMIRKLT"
     gene            5546..6337
                     /locus_tag="PLQ77_04110"
     CDS             5546..6337
                     /locus_tag="PLQ77_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL09892.1"
                     /translation="MFLTRFFRRVINFCLTLGLWVYFMLGYLVLMFVLFIPCYSRLKA
                     VDAVLQKSFHLHLKVFFALVNRLSPGVRFAVSEDVRNLRSSIIVCNHLSYLDPILLVS
                     LFPRQTTIVKNTFFSVPIFGWFLRKAGYVPSSPTEMYGPAMINHLEDIKRQLAAGGNL
                     FVFPEGTRGRRGRIAPFNRGVFSIARYCSTGVKMVLIRNTERLFRPGSFSFDTRGKFT
                     VSMELIGSLTPDQLKNASISASADQARSLFERKIREAKTGGDGRA"
     gene            complement(6616..6918)
                     /locus_tag="PLQ77_04115"
     CDS             complement(6616..6918)
                     /locus_tag="PLQ77_04115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPL09893.1"
                     /translation="MITVIAKLPVKEGKMDEAMAAFQVLISKVASEEEGTVLYSLNRD
                     KANPDVLVVVEQYRDKAALEFHSSTPHFKEFFEASTAFIGGRPEMSVMKEIQRAKP"
     gene            7249..9297
                     /locus_tag="PLQ77_04120"
     CDS             7249..9297
                     /locus_tag="PLQ77_04120"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPL09894.1"
                     /translation="MNPFGIALILLISALLSGSLAVYAWRRRGKPGAGIFFFLMLGTT
                     IAVFAYGMELLSATLSGKLAWVRIRYAGTALIYPMYLLLALWYTNKKKWFAPGRVALL
                     FVLPVFSLIVLFTNSLHHLHYASISLNTSGPFPMIAKTYGPLFAFYFITSFGYVLLAL
                     AILGSNYLVTSPFFRRQTAALMLGTIAPLAAAWLYNTGMVKPLGGLNILPFAYIFSSL
                     TAAWVILGRKLFDLRPFAHGMIFENMTDGFIVLNKQLEILDANPPALRLLDLLPDHMG
                     QFGNKAFVNHPALIRLIHNEPHAHVQIEQDGQVFDAFRTSLFDQQQNLAGHLISLRDV
                     TSIKKAEEALRKSENQYRFLAENMNDVLWMMDLDLKTVYVSPSIEAVLGFTQEERLRQ
                     DVQQQLTPQSLRIARETLAREKVLEKQTGDPKRKATLTLEFCHRDGSTRWLETIISGI
                     RDDQGTLTGLYGASRDVSGRKEAEASILRFSEELREKNKELQCLYSVSEMVRKGQTLQ
                     EDILSACVSLIPQAYDCPEIAGCRIPWEDREYKTENFARTSWMQTSDIRVRGNSIGKI
                     EVCYLEPRPEKDEGPFSKEDRKFLDGIADLLGKSYERRLAAEEREKLIEDLKNALQEV
                     KTLTGMLPICASCKKIRNDQGYWERIERYISERSSVQFSHGICPECMKKLYPEMADHS
                     "
     gene            complement(9335..10336)
                     /locus_tag="PLQ77_04125"
     CDS             complement(9335..10336)
                     /locus_tag="PLQ77_04125"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HPL09895.1"
                     /translation="MRKILTFLLIAILLSGCAAGKSAHIPLEDAGHKDAAYEWAPHDD
                     GGEWWYLTGYAPDQNNKLYFYQFTIFHGYAARLLEAYALHLTFTDCSTGQHVFSESIQ
                     FSSDEVYADSQCVVFQDSRICLDDRRIKIVGASENLKMEIEGTSTKKAAWHGKDGVIV
                     MGHPEEPKERSYYYSFTNIATRGVIEFKDEKGRWVKIDGNGKAWFDRQWGRFSELGWE
                     WFSFRFFDDEEIMLFPFPKTGVKEGTYIDKQGNVAAFDNYAYSVKKFKTFNGKKIGLG
                     WNVTVPFKEKEYEVIPIVEDQYNPSKLQEYWEGICRLQNKKGELVGYTIIETTGSAY"
     gene            complement(10386..12911)
                     /locus_tag="PLQ77_04130"
     CDS             complement(10386..12911)
                     /locus_tag="PLQ77_04130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPL09896.1"
                     /translation="MKICCIAAVCLLSASCATLMDRHFKDKLDATQGTAVLTGLRDTA
                     TVSRDAYGIPFVKANNMEDLAMTVGYVHASDRLAQMIGLKLIAEGRLAEMAGPALLNL
                     DIYMRTMNLKRAAESLYGNVSAENRRLLERYSDGVNAYLDQHKDKLPPGLALSGYKPE
                     PWKPMDSITICVLLSFGLSFNLPEEIAALNIAQAVGAEKTAWLLPIYPDEPIPLGEAA
                     KLRGVDLKKAAESVAGIDELQPFLSSLGLSGAAASNNWAVRGEKTKNGASIFCSDMHL
                     PLSMPSMWNMIHLRCGAYDVAGMSVAGAPMIVAGYNGHIAWGATMVMADNQDLFLEQL
                     RNVNGKLHFLYKGEWLPVVEHQEIFNIKGKPPVARTFRETVHGPLLNEALQAEPIHVI
                     LPASVDLPYGVALSRTVPAADDNSMNAFFQLSFAHSVEDAIPLIKQIRAIPLNMVFAD
                     KDHIAWQVIGNYPVRAKGRGLMPSPGWTGVYDWTGFLDADGLPNAQNPPAGFIGTANH
                     RTVPKDYPHILSSSWYWPERAERIVQMASATDQHTTQTNREMQLDTYSLFVPKLKDAV
                     LKGDLAKEIAREISRWKDEKKAAGARLALAMLRNFSGDMRADSKEAALAGAFLQSATR
                     DIFQDELGPDDSRAWKSFLIADNESYNATCDHLLVRGDESPFWDDIHTPEKETKAQII
                     ALSLADAVAFLESTLGDDTGRWTWGALHTSTWETDTSQMAPHFGFIERTALRSLWSYF
                     NRGPYPAGGDMFTLNVSMYMMGRNFNTWLIPSMRMIVDFSKEEPMLAVNSSGQSDNPS
                     SPHYDDGIGAWRDGKYIPFPFRDAAVRDQYREVMQFRPSAIVR"
     gene            complement(13055..14185)
                     /locus_tag="PLQ77_04135"
     CDS             complement(13055..14185)
                     /locus_tag="PLQ77_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09897.1"
                     /translation="MKKQSPVKNHRVSLTLPAIIFAILFIFAAASCTHLPRPVENIGQ
                     SSEFLDGGKNQPGAKDRARHTLTGKRPFEWWYFDGHLDTGETFVGVFFDPNFTNGKPG
                     ATFSLYSPDWKKMSVLQSLQAEEMKSSTEDVQIACPLGFVRRMDDKQYHVRWNMEGVT
                     ADFKLTMTAPGWMLDGQDESRDFFWSIHQARNRIEGAITQNGQTRQVKGEGYADHNWG
                     RKPLNEITRDWVWGRIFSDEYTIVYADVDYIDPAMNRARPLYIARGDKIIVGAASPTV
                     RQRDFATHPVLKRHYPKQVLIDFEEGGVGAHLNIRFKALVEEVDLLTVAGLNPFSQWI
                     ARTFIARPVYFRIIADYQGDIIEHGKATPIAGECLYEIMGFE"
     gene            complement(14380..15495)
                     /locus_tag="PLQ77_04140"
     CDS             complement(14380..15495)
                     /locus_tag="PLQ77_04140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPL09898.1"
                     /translation="MKIAVIGAGPGGLYAALAAARKNITVDLFEKRKVGEGIVCGECI
                     FDSLGIMTRPGRGLLHPVKGLVLKAGGEYRFPLGSHKPLWMLDRRIWQQDLSRQAKTL
                     GVRLHEQAGVTVSDLVRMKKSYDWILDASGAPSVASRLCGFAREYVQECLAAHQVRLA
                     GDFSSLVPCIRVVFFPDLPAENQPGYAWVFPRDERSANVGVVCTVRKTQGPGRPDMKK
                     MLADFLRREGLAGASVLARGGGIAPARMLSRLVYDNVLLVGDAAGLTSALHGGGIDMA
                     CLSGVLAVKALSGGQAGVARYAGWLERYMREKNALEKVTIGKMRSLTFGEFDRLLSGV
                     TSASLRVRFWTALQNPLMFWTTIRWFKTKKKIPDWPV"
     gene            complement(15492..15827)
                     /locus_tag="PLQ77_04145"
     CDS             complement(15492..15827)
                     /locus_tag="PLQ77_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HPL09899.1"
                     /translation="MGQNPSIADEYKTITAMISLFCRERHQTGGKELCPSCAELLAYA
                     KTRLDKCPFAADKPTCAACPVHCYRPGRRRQIQEVMRCAGPRMLRSHPILAIRHLLKK
                     FKKPGALKP"
     gene            complement(15793..16599)
                     /locus_tag="PLQ77_04150"
     CDS             complement(15793..16599)
                     /locus_tag="PLQ77_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09900.1"
                     /translation="MIQIYEVQKPAEALALIELGVDRIGSVLPDPAKWKSASVRKTVQ
                     AAKAGGATSVLIPIMKDPAAIFAALDYYEPDCVHLCDVLSPWPDERQVAVRDFDALLS
                     LHIDLRDRFPRLDIMRSLSVPRPGLPNASAVEKNILEIAERFAPVTDFFLIDTLLLNA
                     DQPVAGFVGITGETCDWSIAKALIDRSPIPVILAGGLAADNVYDAMTGLRPAGVDSCT
                     RTNATDRSGRPVRFKKDLKKVRRFIEEVRRADLLLEGVPRGAKSIDRGRI"
     gene            16760..17899
                     /locus_tag="PLQ77_04155"
     CDS             16760..17899
                     /locus_tag="PLQ77_04155"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPL09901.1"
                     /translation="MTAPNSIEELYRERGSRARELHDAGEKVIGYFCCFVPDEILAAF
                     DLVPYRIQGSQSESIDEADALLEPMACPFARSCFNLALKGRYDFLDGFVAPHSCDTIE
                     RLYHIWHSNKPSPFNHLINVPHMLGPSSDAFFRKELEYFISRLEEFTGKKLDDGKLRE
                     AIKLYNRRRSLLRELYGLRRESPPRVSGTEITKVLVAGMGIPAAEHIELVEAFIEEVK
                     KRPAPAPDGLPRIFLWGNEIDDVAFVSLVEECGAHVVMDDLCTGSRFFWQDVLETDDP
                     VEALARRYLCTHCPRSLKPQASNRREDLENRFGHIREFMREWKADGAIFYIVRYCDTC
                     ELEGPDLREYLTGLKLPALMIEDDYSTSTIGQLRTRIQAFLEMIL"
     gene            17913..19214
                     /locus_tag="PLQ77_04160"
     CDS             17913..19214
                     /locus_tag="PLQ77_04160"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPL09902.1"
                     /translation="MEQAQAKTTAVKGTDTAREVRALVKNIYKSAHEAKAEGKKVAYI
                     MVASQYDEILRAMDIVPLPTENYAGLCAAKRDMDRFLLKAEADGYSQVLCSYARIGLG
                     FDSLRRELGHIPEGSPDGGMPVPDMMLGSSAVCDPRFKWYQATSRYLEVPTFGIDVVA
                     PPPQADLNAVRDYYIQYQRDQLKGLIEFLETQTGKKMDPDRLWEAIRLGDKAWQLWYD
                     IDRLRVAIPCPMPSQDHFSIMVAGHYYTGTQIAVDFYRKLYDEVKDKVDRKIGVIPDE
                     KHRILYGGGLPPWHTLWIFNYFESFGAVFVIENVYRGFDPVEVPSFVKDPVDFLAWRA
                     FLRFTRYHEKARARSGNPIVEKLLDMIEDYKIDGVVFHACRSCRATTIGQTHIKSELG
                     KYSDLPMMQLVSDMVDLRDYSEAQWKAQIGAFIEALEGRGK"
     gene            19389..20348
                     /locus_tag="PLQ77_04165"
     CDS             19389..20348
                     /locus_tag="PLQ77_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPL09903.1"
                     /translation="MPFAGIDIGSTMTKVVIADEQEKILASHIGPTGAEHRHLALKVM
                     DDTLRSLAHSRAAGVGGVVSSSTYGKSTSASPPPDRLGITPKAEGNDIVKEITLENLD
                     FIVATGYGRINVPFADKQITEITCHARGIRSLFPSARIIIDIGGQDSKGIKLDADGKV
                     ANFVMNDKCAAGTGRFLEVISETLGINLTDMGDLALSADGIVQISNTCTVFAEHEVTS
                     RLAEGAGVAEIVAGLHEAIASRVVNMTRHLGIEKDVVVTGGGAKNKGLIRAIAGKVGF
                     DVLTPPEPFITGALGAALVGRDFYNRGVRPAREREKKEVTFFT"
BASE COUNT         4306 a         6016 c         5794 g         4254 t
ORIGIN      
        1 cgatgatcga catcgaatcc gtgattccgc atcgtgaacc gatcaaaatc atcacggaag
       61 ccatagagct gcaggatgat gccggcaccg cggccgccgt ggtcaatgag cgctggcccc
      121 ttttcgacgg aagaaccgtt cactctctcg tcctggtcga agccgtcgcc cagaccgccg
      181 cgctggtgga aggctacaaa cggaagaaac ggggacagga cagcatcaaa ggctggctgg
      241 tgggcatcaa gtccgcggaa tttcaccggg agcttctcgc cctgcaaacc cggatcacgg
      301 tttcgatcaa aagcctgtat tcctttgacg attatgccgt catcaacggc atcgtcacgg
      361 cggaaaatga aacactgctt acggctgttc tccaggtcgt tcgtctcaac caggattgac
      421 gggtaaaatt caccaaagga gaaaaaaaca tcatggaaga aagaaaaatc gcgctggtga
      481 cgggcgccag ccggggcatc ggacgggcca cggccgtgga actggcccgg accggctgtc
      541 acgtgatcat caactacaac gccagcgaag ccgcggcggc tgaaaccctc gcgcaggtgc
      601 aggcggccgg cgggtccggc gaaatgagaa aattcgacgt gtccgacccg gcggcggtga
      661 aggaagccat ggccgcgatt ctgcaggagc acaaacggat tgacgtgctg gtcaacaacg
      721 ccggcgtcac ggcggacggc cttttcatga tgatgggaga agaggagtgg catcgcgtaa
      781 tccagacaac gctcaacggg ttttttcacg tcacgaaacc cgtcctgcgc gagatggtcc
      841 ggaaaaaacg cggctccatc gtgtccgttt cttccatttc ctccatggtc cccaaccgcg
      901 ggcaggccaa ctacgcggcg gccaaggccg gcatcaacgc cgccaccagc tcgctggcca
      961 aggaagtcgc ccgcttcggc atccgcgtaa acgcggtcgc gccgggcccc atcgccaccg
     1021 atatgctgaa gagcatgccg atggacgaaa gcatcattaa aagcgtcatt ccgatggcaa
     1081 gggttggcaa acccgaagaa gtcgcgcgcg tgatccgctt tctgtgctcc gaggacgcct
     1141 cctacatcac cgggcaggtc atttccgtca acggaggaat gttttgatgc gcgggcaggc
     1201 tggcagacgg ctcttgattt tgctgttttt gttgctgacg gcgtttcccg acgccgccgg
     1261 ggcggatgac tttgaccggc tgcgcaggga cgcgtccggc atctcgtctc tttccgcaga
     1321 ctttgttcag aagaaatcca tgaagatcct gtccgcgccc ctggtctcga aggggaaatt
     1381 ctattatgcg gccccggatt ccatccgctg ggaatatgta aagccgatcc ggagcatcgt
     1441 gatctcgaat caagggagca cgaagcgcta cattgcctcc ggcggcaaaa tgatcgagga
     1501 caaaacgggc ggcgccctgg cgatgaaaat cgtgctcgac gaagtggcgg gctggatgaa
     1561 ggggaaattc accgccaatc cgtcctttgc cgcccgtgtt caggaagggc cggaaacgca
     1621 gattgttctg acgccgacgg ggaaaaacac ggccggcctg attgaaaaaa tcgaaatcac
     1681 cgtttcccgg aaagacgccg tggtgaaaac cgtgaggatt gtggagggcg ccgcggccga
     1741 aacaaggatt gactttctcc acacggtcgt gaaccagacc ctgccccccg gcacctttca
     1801 ggacattcca tgaacctccg tcttcttctg atccttcccc tgctgctggc gtcctgttcg
     1861 tccctgccgg aaatccggcc gtccgccgtt gttgcgggga aggtgatcgc ctgcccttcg
     1921 ccgttcgtcg cccggaaaac cctcttcatc catgcggttg aagcggccgc ggccggcgaa
     1981 acaagggccg tgatgatcgg cgtgacggtg gcggacccgg catcgaggac tctttcctgc
     2041 gccctgatga gcccggaggg catggtcctc tttgaagcct cgcgaaaccc ctccggcacc
     2101 caaatcagcc gggcgctgcc gccctttgac gaggaagaat tcgcccgcgg cctgctcgac
     2161 gatattgaac tgattttttt cgagccgccg gagccggtcg accgggcggg tttttcggac
     2221 acgggccggt ttgtgtgccg gcgccgcctg cagggcggcg ggtggctgga tgtcggaaaa
     2281 gacggcgacg ggcgcgttca aatccgtcgc tactcaaaag gcggcacgct caggcgcagc
     2341 gtggcgctgg cggcaggcgc caatccttac gccgccattg agcttcaggc gtcggaatgg
     2401 atcggctact ctctgtcgat gacgctggtc gaaccggaag ccgttttgga cgaagcgctt
     2461 tcggggaaat aattttcagg aaaggacggc atgcgaaaac cggcagaggg aaaaaatacg
     2521 gtgatcatcg gctcgggcgt cggcggcctt tccgcgggca ttctgctttc gcttctgggc
     2581 tttcgcgtga cggtcgtgga gaaaaacgcc gagccgggcg gcctgatgcg ctcgtattcc
     2641 cgccggggca tcgactgccc cgtgggcgtg cactacgtcg gcgcgctggg gccttccgaa
     2701 ccgctgggca aaatgtttcg cgtgctgggc atcgatccgg acagcctgtt ttcccgcatg
     2761 aaaaacaggg ccgtcatcga ccgctatctt ttcgatgatt tcgttttcga cctgcccgag
     2821 ggtctggacg cctacgaaaa aaatcttcgg gaagcctgtc gggccgacgg cgcagccctg
     2881 gacgcgctga tgaaaggtct gcgcgggacg gccggcgtca tggcggcatc gcccttcttc
     2941 tttgaaggcg gcagtccctt tgccaacatg gattccttcc gtccgatggg agagcttctt
     3001 tgcgaactgg gtgcgtcttc gaaactgcgg gccgttctgg cggccccctg ccagctcgtc
     3061 ggcgtcgaac tcaaagattg cccggtcatc attcatcaaa tggttctggc cggatacctg
     3121 ttttcatcgt ggcgtccgaa aagcgggcgg gatctggcgg acgccttcgc ggcgcggttt
     3181 tcacaactgg gcggaaggct caggctctcc tcggccgtcc ggcatatccg caccaaacag
     3241 ggacgcgtgg ccggagttga tcttgaagag ggggattcac tgcccgcgga ctcggtggtg
     3301 gcggccgttc atcccaaggt tctcatcgga ctgctggatc cggaggcgct cagagagtcc
     3361 cagcgggaga gaatcgcggg cctcgaggaa accggcggcg tccttgccgt tcaggcgggc
     3421 gtggatgccg aggcccatcc cgccgtcgat tacaacctgt accggcttcg ggccggcggc
     3481 agcggagaaa tcgaaaacgg ctttttttgc caggtgctca aaggcgacgg acggaatacg
     3541 aacctgctgt ccatcatcac gggctcgcat taccgggact ggcttccgtg ggaagacacc
     3601 ctgacgagac ggcgcggcga ggcctacgaa cagaaaaaac aggcgctggc cgaagcgctc
     3661 gttgccgaat cgtccgccgt cttcggcccc ctgaaaaacc ttcaactgct ggacgtcttc
     3721 acgcctctct cgctgcgcga ccatgtgagc tgtccggaag ggtcctgcta cggtgttctg
     3781 cgctcctcgc ggcacctgct gaaaatcgcc tcgctcggca accttcccat aggcgggctc
     3841 tacctcgccg gacaaaacgc catggctccc ggggtgatgg ggtgcattct ggggtctctc
     3901 ggcgtggcgc ggaaaatggc ggggaatgag cgtttcggca aggaactcga acggttactc
     3961 taatgctgga cgacctcgcc gatttatgct agctttccgg gcacttttgc cgctaaagga
     4021 gatctatgac acgccaggaa attgaaaagg aaatccgcgc cgtcctgctt agagaattcg
     4081 aggtggaaaa ccccgaaccg gacgtgaacc tgcgggaagc ctacgggttt gacagcatcg
     4141 acgccattga actgcttctg gaaatcgaaa gatttctggg aacggaactg acgcaggcgg
     4201 aaaaaaagaa agccatggac attcggacgc tgaatcagat tatcgactac gtggaaatgc
     4261 tcgcccatca ccggaagaac gaagcggaga aagcataaat gccccagcgc agagtggtca
     4321 tcacggcggc ttcggccatc tcccccatcg ggcacggccg aaagcccatc gtcgaaagcc
     4381 tcgtgaacgg aaaatcgggg gttgcggcac tgcgcgaaga ccatcttatc agccgtttta
     4441 ttcattcccg cgtttacggg accgtcggct atccgatcgc ctatgatttc agccggcagt
     4501 accgcaagac catggggccc gtttcctact acgcctgcca ggttgccagg gaagtcctgg
     4561 aacaggccgg cctgccgccg gattttgtca cgggcggccg gctgggcatc gcgttcgggt
     4621 cgatacacgg ttctcccacc gtgcaaaggg acatatacag gattttcttt gaggcgaagg
     4681 acaaggcgac gtttcagggc atcggggccg ttgattacct gaaatccatg gtgcacacaa
     4741 ccgccgtcaa tatttccaag atgttcggca tcaccggccg catcatttcc tccgggacgg
     4801 cctgcaccac ggcaagccag tccatcggct tcggatacga agcggtcaag tacggcctgc
     4861 aggacgccat gatctgcggc ggcgcggacg aatacgacac gatcacggtg gcggtattcg
     4921 acaatctgct cgcctgctca accgccttca acgaccggcc cggtgagacg ccccgtccct
     4981 ttgaccgtgc gcgcgacggt ctggtggtcg ccgagggggc gggcgccgtg atgctggagg
     5041 aatatgagtt cgccaggaaa cgcggcgcga cgattctggc ggaggtcatc ggttttgcct
     5101 gcaactccaa cggcggcgat ttgattctgc ccaatctcga cggcatcacc aaaaccctgc
     5161 ggctgggcct ggaaaacgca cagatcaacg cctccgacgt cgatctcgtc agcgcccacg
     5221 ccacggccac caagatgggc gacatcatag aggcgcaggc cacgggcagg gttttcggca
     5281 cacaaccgcg ggtggtggcc ctcaagagct acatggggca caccatggcg gcctgcggcg
     5341 cggtcgaaac ggttttgacg atctatatga tgcaggaggg ctttatcgcc ccgacgctgc
     5401 acctggaaga aatcgacgaa cgctgcgcga tgatccggca tgtccagaag ctggaggaaa
     5461 tccccgtcaa aaccgcggcc attcagaatt ttgcctttgg cggcgtgaac acctgcctca
     5521 tgattcgaaa gttgacctga ccgcgatgtt cctcacacga tttttccgcc gcgtcatcaa
     5581 tttctgtctc acgttggggc tctgggtcta tttcatgctg ggctatctgg tgctgatgtt
     5641 tgttttgttc atcccctgct attccaggct gaaggccgtg gacgccgtct tgcaaaaatc
     5701 gtttcacctt catctgaaag tttttttcgc cctggtgaac cggctgtcgc ccggcgtccg
     5761 attcgccgtt tccgaagacg tccggaatct tcgctcctcc atcatcgtct gcaaccacct
     5821 ttcctatctg gatcccatcc tgctggtgtc ccttttcccc cggcagacca ccatcgtcaa
     5881 gaacaccttt ttcagcgtcc ccattttcgg ctggtttctg cgcaaggcgg gttatgtgcc
     5941 ctcctcccca acggaaatgt acggtccggc gatgatcaac catctggaag acatcaaacg
     6001 ccaactggcg gcgggaggca atctgttcgt ctttcccgaa ggcacgcgcg gccgccgggg
     6061 caggatcgcg ccgttcaacc ggggcgtgtt cagcattgcc cggtactgca gcacgggtgt
     6121 aaaaatggtg ctgatccgca acaccgagcg gctgtttcgc cccggatcgt tctcctttga
     6181 cacacgggga aaattcaccg tttcgatgga gctgatcgga tcgctgacgc cggatcaact
     6241 gaaaaatgct tcgatcagcg cgtccgccga tcaggcccga tcgctttttg agcggaaaat
     6301 cagagaagca aaaacaggcg gggacgggcg ggcataagcc cctgcggggg catcagggag
     6361 gaagcatgaa gccaatcacc caccgaatgg cggaggaatg gatcgcggcc gcggcccgca
     6421 ccggcgatcg aattgcatga acgtctgaaa atcagagcgg gctcgacaac ttgcatcccg
     6481 gtttcggcgg gtctaaagac ccgccctacg ttttaatgca gggcgcacct tcaggtgcgc
     6541 ctttgactcc gctatgattc tattttccaa tcaatggcaa atggtcgcgg cctgcctgcg
     6601 gatggcggcc gtttcctacg gcttcgcccg ctggatttcc ttcatcaccg acatttccgg
     6661 gcggccgccg ataaaagcgg tgctggcctc gaaaaattcc ttgaagtgcg gcgtggagga
     6721 atgaaactcc agtgcggctt tgtccctgta ctgttcgacc acaaccagca cgtcgggatt
     6781 ggctttgtcg cggttgagcg aataaagaac cgttccctct tcctcggaag ccactttgga
     6841 gatcagcacc tggaaggcgg ccatggcttc atccatcttc ccctctttca caggcagctt
     6901 ggcaataacg gtaatcatcg taacacctcc cgaatggttt ttttgaagca ttttcgactc
     6961 gattcgcccc catagcacaa acacgctcaa agtggcaagc ccttcggaag gccaaaacgg
     7021 cggcggtgag gccgttcccc tgcagccggc tggtttccgc gaaccggacg gcatggttaa
     7081 aaaaatgatt ttctgctcga aagaattgaa aaaacagata aaatatgcaa tggataccat
     7141 caactcatga ttgcatttgc ccaaacgact tgaaattgtt gcaggggttg atcgatgcgc
     7201 gggcagcaaa atccgtcgcc ctgaaatgac ttaaggaaat tcattttcat gaatcccttt
     7261 ggaatcgcat tgattttatt gatttccgcc ctcctctccg gcagcctcgc ggtctatgcc
     7321 tggcgccgac ggggcaaacc gggagccggc attttctttt ttttaatgct ggggacgacg
     7381 atcgcggtct ttgcctacgg gatggaactc ctttccgcca ccctttcggg caaactggcc
     7441 tgggtgagaa tccgttatgc gggaaccgcc ctgatctatc cgatgtacct gctgctcgcg
     7501 ctttggtaca ccaacaagaa aaaatggttt gctcccggcc gtgtcgccct gctgtttgtc
     7561 ctgccggtct tctccctgat cgtgcttttc accaattcac tccaccatct gcactatgcc
     7621 tcgatcagcc tgaatacaag cggccccttc cccatgatcg ccaagacata cgggcccctg
     7681 tttgcgtttt acttcatcac ctcctttggt tatgttttgc tggcgctggc catcctggga
     7741 tcgaactatt tggtcacgtc gccttttttt cggcggcaaa cggcggcgct gatgctggga
     7801 accattgccc ccctggcggc cgcctggctg tataacaccg gcatggtgaa accccttggc
     7861 ggattgaata tccttccttt tgcctatatc ttttccagcc tgacggccgc atgggtcatc
     7921 ctgggccgca agcttttcga tctgcgcccc ttcgcccacg gaatgatttt tgaaaacatg
     7981 acggatggat ttattgtcct gaacaagcag ctcgaaattc tggacgccaa tccccccgca
     8041 ttgcgcctgc tggacctttt accggatcac atgggtcaat tcgggaataa ggcgtttgtg
     8101 aatcacccgg cgctgatccg gctgattcat aacgaacctc acgcccacgt gcagattgaa
     8161 caggacggac aggtcttcga cgcattccga acttccctct tcgaccagca gcagaatctg
     8221 gccggccatt tgatcagcct gcgcgatgtc acctccatca agaaggcgga ggaggccctc
     8281 agaaaaagcg aaaaccagta ccggtttctg gccgaaaaca tgaacgacgt cctgtggatg
     8341 atggatctgg atctcaaaac cgtctatgtt tctccctcta tcgaggccgt cctcggcttc
     8401 acgcaggagg aacgccttcg tcaggacgtt cagcagcagt tgacgccgca atctctcagg
     8461 attgcacggg agacgctggc ccgggaaaaa gtgctcgaaa aacagacggg cgatcccaaa
     8521 cgaaaagcaa ccctgacgct ggaattctgt caccgggacg gctccacccg ctggctggag
     8581 acgatcatca gcggcatccg tgatgatcag ggcacgttga ccggcctgta cggcgcatcc
     8641 cgggacgttt ccgggcgcaa ggaagcggag gccagcatcc tccggttttc ggaggagctc
     8701 agagaaaaaa acaaggagct tcagtgtctc tattccgtgt cggagatggt tcgaaaagga
     8761 caaaccttgc aggaagacat tctttcagct tgcgtctccc tcatcccgca ggcctatgat
     8821 tgcccggaga tcgccggctg ccggattccg tgggaagacc gggaatacaa gacggaaaac
     8881 ttcgcccgga cctcctggat gcaaacgagc gacatccgtg tgcgcggcaa ttcgattgga
     8941 aagatcgaag tctgctacct tgagccgcgg ccggaaaaag acgaaggccc tttttcaaag
     9001 gaagatcgca agttcctcga cggcatcgcc gacctgctgg gaaaatcata cgaacggcgg
     9061 ctggccgccg aagagcggga aaagctcatc gaggatctta aaaatgccct gcaggaggtc
     9121 aagaccctga ccggcatgct tccgatctgc gcgtcgtgca aaaaaatacg aaacgatcag
     9181 ggatactggg aaagaatcga gcgctacatc tccgagcgtt catcggtcca gttcagccac
     9241 ggaatctgcc cggagtgcat gaaaaaactg tatccggaaa tggcggacca ttcctgagcg
     9301 cggggaatgc ttccggaccg atcctgcctg cccgtcagta ggcgctcccg gtggtttcaa
     9361 taatcgtgta cccgacaagc tcgccttttt tgttttgcag tctgcagatc ccttcccagt
     9421 attcctgcag ctttgacgga ttatactggt cctcaacaat gggaattacc tcgtattcct
     9481 tttccttaaa gggaacggtc acgttccatc ccagtccgat ctttttgccg ttgaacgttt
     9541 taaacttctt tacgctgtag gcataattgt caaaggcggc aacgtttcct tgtttgtcta
     9601 tgtaggttcc ttctttgacg cctgttttgg gaaaaggaaa gagcatgatc tcctcgtcat
     9661 caaagaatcg gaaggaaaac cattcccagc ccaactcgct gaacctcccc cactgccgat
     9721 cgaaccacgc cttgccgttc ccgtcgatct tgacccaacg gcccttttca tccttgaatt
     9781 cgatcactcc ccttgtggca atattggtaa aggaatagta gtagcttctc tcttttggtt
     9841 cttcgggatg ccccatgacg atgacgccgt ctttcccatg ccacgcagcc ttcttcgtgg
     9901 acgtgccttc aatctccatt ttgagatttt cgcttgcgcc cacgattttg atccgccggt
     9961 catccaggca gatccggctg tcctgaaaga caacgcattg tgaatccgca tagacctcat
    10021 cagagctgaa ttgaatggat tctgaaaaaa catgctgtcc ggttgagcaa tccgtgaacg
    10081 tcaaatggag cgcatacgct tccaggagac gtgccgcata tccgtggaat attgtaaatt
    10141 gataaaagta caatttgttg ttctgatccg gcgcataccc ggtgagatac caccactctc
    10201 ctccgtcgtc atggggggcc cattcataag ccgcgtcttt gtgacccgca tcctccaatg
    10261 gaatatgggc tgatttgccc gcagcgcaac ccgacagcaa aatcgcgatc aacaggaatg
    10321 tcagtatttt tctcacagga tcctcctttt gcattgagtc ttgccgcttc gttttgcagg
    10381 ggaagctacc gcacgatcgc ggatggcctg aattgcatca cctctcgata ttgatccctg
    10441 acggcggcgt cccggaaggg gaatggaata tactttccat cccgccaggc cccgatgccg
    10501 tcatcatagt gagggctgga aggattgtcc gactggccgg agctgttcac cgccagcata
    10561 ggctcttcct tactgaaatc cacgatcatg cgcatggaag gaatcagcca ggtattgaaa
    10621 tttctgccca tcatgtacat ggatacattc agcgtgaaca tgtctccccc ggcgggatac
    10681 ggaccccggt tgaagtatga ccacaaactc ctcagcgccg tccgttcaat aaagccaaaa
    10741 tggggcgcca tttgagaagt atcggtttcc caggtgctgg tatgcagcgc tccccatgtc
    10801 caccggccgg tgtcatctcc caaagttgat tccagaaatg cgacggcatc cgcgagcgaa
    10861 agggcgatga tttgcgcttt ggtctctttt tctggcgtat ggatatcgtc ccagaagggg
    10921 ctttcatcgc cccggaccag gagatgatcg caggtggcgt tgtagctctc attatcggcg
    10981 atcagaaacg acttccacgc cctggaatca tccgggccga gctcgtcttg aaaaatgtct
    11041 ctggtggcgc tttgcaggaa cgctcccgcc agggcagcct ctttggaatc cgccctcata
    11101 tcgccgctga agttccgcag catggcaagg gcaagacgcg cgcctgccgc ctttttttca
    11161 tctttccacc ggctgatttc ccttgcaatc tctttggcca gatctccttt caacacagcg
    11221 tccttgagct tcggcacaaa cagggagtag gtgtccaact gcatctcccg gttggtttgg
    11281 gtggtgtgct gatccgtcgc cgacgccatc tggacgatgc gctcggccct ttcgggccag
    11341 taccaggaag aggaaaggat atggggataa tccttcggca ccgtgcggtg gttggcggtt
    11401 ccgataaatc cggccggagg gttttgggca ttcggcaatc cgtcggcatc caaaaagccg
    11461 gtccagtcgt aaacgccggt ccagcccggc gaaggcatca gcccgcggcc tttggcccgg
    11521 acggggtaat tgccgatcac ctgccaggcg atgtgatcct tgtccgcaaa taccatgttc
    11581 agcggtatgg ctctgatctg ttttatcagc ggaatcgcat cctccaccga gtgggcgaaa
    11641 ctgagttgaa agaaagcatt catgctgttg tcatctgcgg cgggaaccgt tcggctcaac
    11701 gccacgccgt aaggcagatc gacggaggcc ggcaaaatga catgaatcgg ctccgcctgc
    11761 agcgcttcgt ttaaaagcgg cccgtgcacg gtttcgcgaa aagtccgggc aacgggcggc
    11821 ttgcctttga tgttgaatat ttcctgatgt tcgacaaccg gcagccactc tcctttgtat
    11881 aaaaaatgca gtttcccgtt cacgttcctg agctgctcca ggaacaagtc ctggttatcc
    11941 gccataacca tggtcgcgcc ccacgcaata tggccgttgt atccggcaac gatcatcggg
    12001 gcgcccgcaa cactcattcc ggccacgtca taggcgccgc accggagatg gatcatgttc
    12061 cacatggacg gcatgcttaa aggaagatgc atatcgctgc agaaaatgct cgcgccgttt
    12121 tttgtttttt ccccgcggac ggcccaatta ttggaagcgg cggcgccgct caagcccagg
    12181 gagctcaaaa acggctgcag ttcatctatt cccgccacac tttcggcggc cttcttcaag
    12241 tcaacgcccc tgagcttggc ggcctccccg agtggaatgg gctcatcggg atagatgggc
    12301 agcagccagg ctgttttctc ggcgcccacg gcttgagcga tgttcaaggc ggcaatttcc
    12361 tccggaagat taaaggaaag accgaagctc agcaaaacgc atatcgttat ggaatccatg
    12421 ggcttccagg gttcaggctt ataccctgac agcgcaagac ccggcggcag tttgtctttg
    12481 tgctgatcca gataagcatt tacgccgtcg ctgtatcgct ccaaaaggcg gcggttctcg
    12541 gcggaaacat ttccgtaaag actctccgcc gctcttttga gattcatggt gcgcatgtaa
    12601 atatccagat tcagcaaggc tggtccggcc atctcggcca gccggccctc cgctatcagt
    12661 ttcaagccga tcatctgggc gagccggtcc gaggcatgca catagcctac ggtcatggcc
    12721 aagtcttcca tgttgtttgc tttcacgaag ggaatgccgt aagcgtcgcg gcttaccgtt
    12781 gcggtatccc tgagccccgt caggaccgcc gttccctgag tggcgtccag tttgtctttg
    12841 aaatgccggt ccatcagcgt ggcgcagctt gcagagagaa gacagacggc ggcaatacag
    12901 cagattttca agaattgctt ccccggatga ccccgtctca ttccaggatg tgcggtaggg
    12961 ttctgcatcg gacctcctca aatcattttc caaaatgttt ttgcgatccg ttgatgaatc
    13021 cggcggagag atcagccccg ttcacggcgg agcgctactc gaagcccatg atctcataga
    13081 ggcattcacc tgctatgggg gttgccttgc cgtgctcgat gatgtcgccc tggtaatcgg
    13141 caataatccg aaaatagacg ggccttgcga taaacgttct ggcgatccac tgagaaaagg
    13201 gattcaatcc cgcaacggtg agaagatcaa cctcttccac cagagccttg aaccggatat
    13261 tcagatgcgc cccaacgcct ccctcctcaa agtcgatcag gacctgcttc ggataatggc
    13321 gcttcagaac ggggtgggtg gcgaaatccc gctgccggac cgtagggctg gcagcgccca
    13381 cgatgatctt gtcccccctg gcgatataca gcggccttgc cctgttcatg gcgggatcga
    13441 tataatcaac gtccgcataa acaatggtgt actcgtccga gaagatcctt ccccataccc
    13501 agtcccgtgt gatctcgttg aggggttttc ttccccagtt gtggtcggcg tatccctctc
    13561 cttttacctg cctggtttgc ccgttttgcg tgatggcgcc ctcgatgcgg ttgcgggcct
    13621 gatggatgct ccagaaaaag tcccgggatt cgtcctgacc gtcgagcatc catcccggcg
    13681 ccgtcatggt cagcttgaaa tccgccgtca cgccttccat gttccaccgg acatggtatt
    13741 gcttatcgtc cattcttcga acaaacccca gggggcaggc aatctgcaca tcctccgtcg
    13801 aggatttcat ctcttccgcc tgcagggatt gcagaacgga cattttcttc cagtccgggc
    13861 tgtagagcga aaaggtggcc ccgggttttc cgttggtaaa gttcgggtcg aagaataccc
    13921 cgacaaaggt ttcaccggta tccaaatgcc cgtcaaagta ccaccactca aagggacgct
    13981 tgccggtcag ggtatggcgc gccctgtcct ttgcgccggg ctggttcttg cccccgtcca
    14041 gaaactcact gctttggccg atgttctcga cgggcctggg cagatgggtg caggaagcgg
    14101 cggcaaagat gaacaggatg gcgaaaatga ttgccggaag cgtcaacgat acgcggtggt
    14161 ttttgacagg ggattgtttt ttcatatgcg tcctataaga atttcgaacc cggctgtcaa
    14221 gaaaatcttg ctcgccatgc atcctgcgcg aatgctgcgc atgaccaatt gaaatgccgc
    14281 atgacttttg aaaatcaaaa caccgcggca ggaaaagcgt tcctgatccc tggacaggct
    14341 gcttttgctt ccgcaagcca tacggggcaa aacgggtgtt caaaccggcc agtcgggtat
    14401 tttcttcttc gttttgaacc accggattgt cgtccagaac atcagcggat tttgcaaagc
    14461 ggtccaaaag cggacccgca ggctcgcgga ggtcacgccg gagagcagac ggtcgaactc
    14521 gccgaaggtc agggagcgca ttttgccgat ggtgactttc tccagggcat ttttctcgcg
    14581 catgtagcgt tcgagccatc ccgcatagcg ggccacgccg gcctgccccc cgcttaaagc
    14641 cttcacggcc agcactccgc tcaagcaggc catgtcgatg ccgccgccgt gcagcgcgga
    14701 ggtaagcccc gccgcgtcgc ccaccagaag aacgttgtcg taaacgagac gggaaagcat
    14761 tcgggcagga gcgatgccgc cgcctcttgc cagaacggat gcgccggcaa gtccttcccg
    14821 gcgcaagaag tcggccagca ttttcttcat atccggccgt ccgggtccct gcgttttgcg
    14881 caccgtgcag accacgccga cgttggcgct tctctcatcc ctgggaaaaa cccaggcgta
    14941 gccgggctga ttttcggccg gaagatccgg gaaaaagacg acccgaatgc agggcacaag
    15001 cgagctgaaa tcgccggcca gcctcacctg atgcgccgcg aggcactcct ggacatactc
    15061 gcgcgcaaac ccgcaaagcc gggacgccac ggacggagcg ccgctggcgt ccagaatcca
    15121 gtcatacgat ttcttcatcc gaaccaggtc cgacaccgtc acgcccgcct gttcgtgcag
    15181 gcgaacgcca agcgtcttgg cctgacggga aagatcctgc tgccagatcc ggcgatcgag
    15241 catccacagg ggcttgtgac tccccagggg aaagcggtac tcgccgccgg ctttcaaaac
    15301 aagccctttt acgggatgca gcagtccccg gcccggccgg gtcataattc ccagggaatc
    15361 gaagatgcat tcgccgcaga cgatgccttc cccgaccttc ctcttctcaa acaggtccac
    15421 ggtaatgttt ttgcgggccg ccgccagcgc ggcgtaaagg ccgccgggtc cggcgccgat
    15481 gacggcaatc ttcatggctt tagcgctccg ggctttttga attttttcag aaggtggcga
    15541 atcgccagaa tcggatggga gcgcagcatg cgcggaccgg cgcagcgcat cacctcctga
    15601 atctgccgtc tgcgcccggg cctgtagcaa tgcaccgggc aggccgcgca ggtcggctta
    15661 tccgcggcaa acgggcactt gtccaggcgc gttttcgcgt aggcgaggag ctcggcgcac
    15721 gacgggcaca actctttccc gccggtctga tgccgctccc ggcagaacag ggaaatcatc
    15781 gccgtgatgg ttttatattc gtccgcgatc gatggatttt gccccacggg gaactccttc
    15841 aagaagaagg tcggcgcggc gaacttcttc gatgaaccgc cgaactttct tcaggtcttt
    15901 tttaaagcgc acgggccttc cgctccggtc ggtcgcgttg gtcctcgtgc agctgtccac
    15961 accggccggc cggaggcccg tcatcgcgtc gtaaacattg tcggcggcca gtcctccggc
    16021 cagaatcacc ggaatggggc ttcggtcgat taaggctttg gcaatagacc agtcacaggt
    16081 ttcccccgtg atgccgacaa agcccgccac cggctggtcc gcgttcaaaa gcagcgtgtc
    16141 gatcagaaag aaatccgtca ccggcgcgaa gcgctccgca atctccagaa tatttttttc
    16201 gacggcggag gcgttgggca acccgggccg gggaacggac agagaccgca tgatgtcgag
    16261 gcgcggaaac cggtcccgaa ggtcgatgtg caggctcaaa agcgcgtcga agtcccgcac
    16321 cgcgacctgc ctctcgtcgg gccagggcga aaggacatcg cacaggtgaa cgcaatccgg
    16381 ctcatagtag tccagcgccg cgaagatggc cgccgggtct ttcatgatcg gaatcagaac
    16441 gctggtcgcg ccgcctgcct tcgccgcctg cacggttttg cggacggacg cgcttttcca
    16501 ttttgccgga tcgggaagca cgctgccgat ccggtccacg ccgagttcga tcaatgcgag
    16561 ggcttccgcg ggcttttgca cttcataaat ctgaatcatc gttttccgca tggtaatgac
    16621 ttatcatgac cgccgcgccc aatcaagatc tgcgacggag tgatttccgc ccgccgggca
    16681 aggtcaaaaa gcaacttgct tttatccgcg tttcactgaa taatcgggcc cattcaaaaa
    16741 cgaatacagg gggttttgca tgacggcacc caactccatc gaggaactct acagggaaag
    16801 aggctcccgc gcgcgcgaac tgcacgacgc gggggaaaaa gtcatcggct acttctgctg
    16861 ctttgtgccg gatgaaatcc tcgcggcctt cgacctggtg ccctaccgca tccagggctc
    16921 gcaaagcgaa tccatcgacg aggcggacgc gctcctggag ccgatggcct gcccgtttgc
    16981 ccgaagctgc ttcaacctgg cgctcaaggg ccgctatgat tttctcgacg ggttcgtcgc
    17041 cccgcacagc tgcgacacca tcgagcgcct gtatcacatc tggcacagca acaagccctc
    17101 ccccttcaat cacctgatca acgtgccgca catgctgggc ccgtcttccg acgctttttt
    17161 ccgcaaggag ctggaatact ttatttcccg cctggaggaa ttcaccggga aaaagctgga
    17221 cgacggcaaa ctgcgcgaag cgataaagct atacaaccgc cggcgttccc tgcttcggga
    17281 gctttacggc ctgcgccgcg aaagcccgcc gcgcgtttcc ggcacggaaa tcaccaaagt
    17341 tctggtggcc ggcatgggca tccccgccgc ggagcacatc gaactggtgg aggcgtttat
    17401 cgaagaggtg aaaaagcgcc ccgcgccggc gccggacggc ctgccgcgca tctttttgtg
    17461 gggcaacgaa atcgacgacg tagcctttgt cagcctggtc gaagaatgcg gcgcgcatgt
    17521 cgtgatggac gatctgtgca ccggatcgcg gtttttctgg caggacgtct tggaaacgga
    17581 cgatccggtg gaggcgctcg cccggcgcta tctctgcacg cactgtcccc gaagtctgaa
    17641 gccgcaggcc agcaaccgca gggaggacct ggaaaaccgc ttcggccaca tccgcgaatt
    17701 catgagggag tggaaggcgg acggggcgat cttttacatc gtccgctact gcgacacgtg
    17761 tgagctggaa gggccggatc tcagggaata cctgaccggt ctgaagctgc cggcgctgat
    17821 gatcgaggac gactattcga cctcgaccat cggccagttg cgcacccgca tccaggcatt
    17881 tttggaaatg atcctgtagg gaaaggaatt ttatggaaca ggcgcaggct aaaaccacgg
    17941 cggtgaaggg aacggacacg gcgcgggaag tgcgcgcgct cgtgaaaaac atttacaaaa
    18001 gcgctcacga ggccaaagcc gaaggcaaga aagtcgctta catcatggtt gcctcgcagt
    18061 acgacgaaat cctgcgcgcc atggacattg tgccgctgcc cacggaaaac tacgcgggcc
    18121 tgtgcgccgc caaacgcgac atggaccgct ttttgctgaa ggccgaagcc gacggctact
    18181 cgcaggtcct gtgcagctac gcccgcatcg gcctgggctt tgacagcctg cgcagggaac
    18241 tgggacacat tcccgaagga agccccgacg gcggcatgcc ggtccccgac atgatgctgg
    18301 gcagctccgc ggtgtgcgat ccgcgcttca agtggtatca ggcgacgagc cgctatctgg
    18361 aagttcccac cttcggcatc gatgtggtcg ccccgccgcc gcaggcagac ctgaacgccg
    18421 tccgcgacta ctacattcag tatcagcgag atcagctcaa aggcctgatc gaatttctgg
    18481 agacgcagac cggcaaaaaa atggaccccg accgcctctg ggaggcgatc cgcctgggag
    18541 acaaagcctg gcagctctgg tacgacattg accggctgcg cgtggcgatc ccctgcccga
    18601 tgccgtcgca ggatcacttc agcatcatgg tcgccggcca ttactacacg ggcacgcaga
    18661 tcgccgtgga cttctaccgg aagctctacg acgaagtgaa agacaaggtg gatcggaaaa
    18721 tcggcgtcat tccggacgaa aaacaccgca tcctttacgg cggcgggctc ccgccctggc
    18781 acacgctgtg gattttcaac tactttgaaa gcttcggcgc ggtgttcgtg atcgaaaacg
    18841 tttaccgcgg cttcgatccg gtggaagtgc cctctttcgt caaggatccg gtggattttt
    18901 tagcctggcg ggcatttctg cgcttcacgc gctaccacga aaaggcccgc gcgcgcagcg
    18961 gcaatcccat cgtggaaaag ctgctggaca tgatcgaaga ctacaaaatc gacggcgtgg
    19021 tctttcacgc ctgccggtcc tgccgggcca ccaccatcgg ccagacgcac atcaaaagcg
    19081 aactgggcaa gtattccgat ctgccgatga tgcagctcgt ctccgacatg gtggatttgc
    19141 gggattattc ggaagcgcag tggaaagcgc agatcggagc gtttatcgaa gcactggaag
    19201 gacgaggaaa atagtggatg gtggatagtg aaaagcgatg agcggaaagt gtcatggttc
    19261 gacaagctta ccactcaaaa tgctcaaaca ctcaacaagc aatgtcacct cgatgcttcg
    19321 actggctcag catgacattg cttgtcgagg gacaccatcc actatcaact atccaccaaa
    19381 ggtgaatgat gccgtttgca ggaattgaca tcgggtccac aatgacgaaa gtggtcatcg
    19441 cggacgaaca ggaaaaaatc ctggcctcgc acatcgggcc gacgggcgcg gagcaccgcc
    19501 atctggcgct taaggtcatg gacgacacgc tgcgttccct cgcgcattca cgcgctgccg
    19561 gcgttggtgg cgtcgtcagc tcctcgacgt acgggaaaag tacgtctgcg tcgcctcctc
    19621 ctgaccgcct tggcatcacc ccgaaagcgg aagggaacga tatcgtaaaa gaaattaccc
    19681 tcgaaaacct cgatttcatc gtcgcgaccg gctacggccg catcaacgtt ccttttgccg
    19741 ataaacagat cacggaaatc acctgccacg cgcgcggcat tcgctctctt tttccgtcgg
    19801 cgcggatcat catcgacatc ggcggccagg attccaaagg catcaagctc gacgccgacg
    19861 gcaaagtcgc caatttcgtc atgaacgaca agtgcgccgc cggaaccgga cgctttctgg
    19921 aagtcatctc ggagacgctg ggcatcaacc tgacggacat gggcgacctc gctctttcag
    19981 ccgacggcat cgtccaaatt tccaatacgt gcacggtatt cgccgagcat gaagtgacct
    20041 ctcgcctggc cgaaggagcg ggcgtggcgg aaatcgtggc ggggcttcat gaggccatcg
    20101 cgtcccgcgt cgtcaacatg acgcgccacc tgggcatcga aaaggacgtt gtcgtcaccg
    20161 gcggcggagc gaaaaacaag ggactgatcc gggccatcgc aggaaaggtc ggctttgacg
    20221 tcctcacgcc gcctgagccg ttcatcaccg gcgccctggg cgcggcgctc gtcggcaggg
    20281 atttttacaa ccggggcgtc agaccggcaa gagagcggga aaagaaggaa gtgacctttt
    20341 ttacttaaca ccttttgaag atgtatccgt
//
LOCUS       DAOPAF010000025        19555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2899_length_19555_cov_47.994205, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000025 DAOPAF010000000
VERSION     DAOPAF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_19555_cov_47.994205"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1188
                     /locus_tag="PLQ77_04170"
     CDS             145..1188
                     /locus_tag="PLQ77_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09904.1"
                     /translation="MKPIVKMTLAMAAVLFFTSCAAGHNACLKSRMDRHEVRSPAFWQ
                     DYRRGNLEDRLHAAPEPLLDYLHLDNRLNGYDTRPQATVADDSFRQDVRQAMAELPGP
                     VREAVKEKLIGIFFVRDLGTTGYTEAVADPQGRPAAGFVVLDVGSLRQTANAWATSRE
                     NTVFQNDGEGRLQVTLEEAPNDNSKNAIQFILLHEFGHLVSIGERFHPSWLDEAKPAG
                     AIEDYLFYPLSWRKEKDGSNVSLFEDVFPERRDVRFYGEARLKSPQMADVYRRLAMTN
                     FVSLYGATGPFEDFAESFALYVHSVWMKKPYRVQITQSGRPVFLYESCWDQPRCAAKR
                     AVLEEWFSRFRKP"
     gene            complement(1209..1973)
                     /locus_tag="PLQ77_04175"
     CDS             complement(1209..1973)
                     /locus_tag="PLQ77_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPL09905.1"
                     /translation="MTTHENRSLVPAAVIVGVCLLAGLLLGGYFIGKGVARFKSDTRT
                     VTVKGLVEKEVKADQAIWTLNFRRAGEDLKDVHAKIIADRDATIAFLKKQGFKNEEVT
                     WQPSKTLDKLAREYGQNEKNDRFRYVVTSSLRVTTKNIDMVIRALGATEELLKAGILL
                     DGQQGEGMANPRYVVTTFNALRPQLLAEATKNARFTAEQFASDSGARIGKIRSANQGM
                     IQIFGSEGNDESAPYSPTSTPVKKIRVVSTFEFELK"
     gene            complement(1996..2706)
                     /locus_tag="PLQ77_04180"
     CDS             complement(1996..2706)
                     /locus_tag="PLQ77_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09906.1"
                     /translation="MKEFDEESLAQFDGRDGRPCCIAHGDRVIDVSASKLWKTGSHMK
                     RHQAGSDLTLAIGAAPHGTDVLDKYPQIGIFKKKNAKPFLPAPLEALMERVPFLRRHP
                     HPMVVHFPMVFSMAPVFFYLLYLLTGERSFEATALHCLAADILFIVPAIATGFLTWWV
                     NYQAKFMKPVRIKIYASFILLAVAATAFLWRILRPEAFAAFGTEAVIYFILLLSMAVL
                     VSIIGFFGGGLTFPAEKK"
     gene            3202..4587
                     /locus_tag="PLQ77_04185"
     CDS             3202..4587
                     /locus_tag="PLQ77_04185"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPL09907.1"
                     /translation="MGDRMFIMFVHLVQNLAVLMVMAYVTTRIPWFGDLLNRRFSLKN
                     SLLLGLVFGLISIFGTLSAVKTFGALANFRDLGPAIAGLLAGPVAGGVAGLIGGMHRY
                     SLGGITGVPCSIATVLAGLICGGFYLWKKGRPIRIREATAVMAVVTILHGFVITPLIL
                     GLSEEIRQILRTVMLPMLLVNCAGIALFFFILHNLNRERLNEAEKHRIDSELRIAREI
                     QMGMLPAPSQNGDPRFAVHAVLKPAKEVGGDLYHFFAKDNDRICLAVGDVSGKGISAS
                     LHMAITQKLIKALALSGEEPAELLGRLNRELCDGNESMMFVTLFVAFIHLESGKTVFG
                     NGGHNPPYRIRRGDVSRMVLEPGLALGIRENAVYTNQRLCLEAGDTLFLYTDGVTEAM
                     NGRKELFGEKRLAAALSRLRDSDPEGICRGLLEELAHFVGQSEPSDDITMLAVRLTEK
                     SNAAAGAVRGG"
     gene            4625..4957
                     /locus_tag="PLQ77_04190"
     CDS             4625..4957
                     /locus_tag="PLQ77_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPL09908.1"
                     /translation="MKVWSKRQQDVVVLAVQGRLDAATAPLFEEQVLPMISAGDKNFL
                     LDLAGLDYISSAALRRLLFFAKRAEDQGGRVVLLSVQNHILEILDMAGFTQLFAIFAD
                     QDEALRSF"
     gene            4969..6678
                     /locus_tag="PLQ77_04195"
     CDS             4969..6678
                     /locus_tag="PLQ77_04195"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPL09909.1"
                     /translation="MPRKPSNLIYGVDENPGPIALVLMGLQHISLMSIAFIFPVIIIE
                     AIGGNLEEAEGLIRMAMLATGIGTILQGINRGPVGSGYLCPLLNGPAFLSASLMAGKL
                     GGLSLIFGMTAVAGVFEAFFSRLLSRMRAVFPAEVTGTIVTMVGIEVVPLSVRKFLGI
                     DPTHSMPDSTAVLIAMITLGAMACFNVWGKGKLRLYSVLLGMIVGYAASAMAGLLSPD
                     TFRVMMEAPFFALPAVGHYGFSFEWVLLVPFLVAALSSALKTMGDVTTCQKINDAQWK
                     RPDMVNISGGVLACGISNILSGMLGALGQSVSSSNIGLSIATGATSRRIAYSTGIILI
                     LLAFFPKFASIFVIMPTPIMGASLIFAVSFMILAGLQIIMSRMIDARKTFVIGISIIF
                     GLSVEFAPGLYRDVHPWIKPIFSSSLSLATICAVVLNLIFRIGIAKTTSLVLTPGENP
                     SEDIDHFMEEQGRAWGARSEVIYKAKAAMIEFYEAAAESALTRKPVTMTARFDEFNLD
                     VDMDYEGKPIELAASRPDARDLLTDPDAPLKLAGYMIRSLADHAAMESVAGRNRVHLH
                     FNH"
     gene            complement(6774..7976)
                     /locus_tag="PLQ77_04200"
     CDS             complement(6774..7976)
                     /locus_tag="PLQ77_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09910.1"
                     /translation="MTYYVLIAFCVIILLAYIFDITAKYTRIPGVILLIATGMFINYL
                     ASSFKIGIPDMSGMLPIMGTLGLILIVLEGSLDLAIRRDKSSLIIGSVSSAIVLFAVS
                     IGVFTYVAVYFLHYDLQASLLNIIPFAVISSAVAIPSAASLNPEDREFVTYESSISDI
                     IGILAFDFILLSSGSLGAGVLRFIAELILTFIASILLSGGLAFLLHKISHHVKYVIIM
                     TAIILVYALAKLVHLPSLLVVLIFGLVMNNNVLFKNEFSAKWINFDEFNADLGAFKNI
                     VFELTFIIRCFFFILFGFYTRLSDLLNLNNLMIAAAITASIFIFRALFFRIVLRLPLN
                     PLLYFAPRGLITILLFLSIPSILLLPFINVGIVTQVIFLTILMMMFGNMIFSKDKTPE
                     APETPIRR"
     gene            complement(7995..8675)
                     /locus_tag="PLQ77_04205"
     CDS             complement(7995..8675)
                     /locus_tag="PLQ77_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPL09911.1"
                     /translation="MRYIVIGLGFFGSSLAAKLTSLGHEVIGIDRRSERAEELKDKIT
                     NVMIMDCTKPAALSALPLSDVDAVIVAVGEDIGSSVLILSMLKKQKVKRIIGRAINPM
                     HQNILGELGIEEIIHPEEDTATELTAMLMVQNAVNTMIVNDKIIIAELKVPDAYVGHT
                     LEAANLENRFGIRLVAVKIAPAQKSLSTLLGKEYEVDLSGDPHRPLGAKDRLIVIGRL
                     NDIRRFVD"
     gene            complement(8684..10498)
                     /locus_tag="PLQ77_04210"
     CDS             complement(8684..10498)
                     /locus_tag="PLQ77_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPL09912.1"
                     /translation="MSYFDNEALFKKFYVASDWANTVGSAIIALAGFVFALSTLLFGS
                     LILFDFGFRKTAADSLAVHQTYLLLAAALFASKLLIELLAFRAAKWFAVVFRVLVLAV
                     VFVILLAHLHAFPEAWSVISGALGGRTALLLGSLALMATQMHHLSGTLNRINISPSFL
                     FAGSFLAMILAGAGLLMLPNATLQPISAYEALFTATSAVCVTGMMIVDPSAVFSPMGK
                     NIILGLIQTGGLGIMTFTGFFSYLFLGSASLKDRFLLKDFFSGDQLGGLYKLLLKILL
                     LTILVEAAGSVLIYRTLEGDWMHKTHNAVFHAVSAFCNAGFSVNPQEFNALHLQSNFS
                     LQFILCGLVVLGGIGFPLLLYFYKVLKFNVKSIFFRVVGRNLPHEVLLLAVGERLALH
                     ATLVLLVLGAFLYYALEAGSPLPEHFNWSQGMTALVSSVNARTSGFSMTDPGGWSYPT
                     VFLTIFLMWVGASPGSTGGGIKTTTLAVAVKTVASFLRGKDRLEIGNREIGSSTIVRA
                     FSVIVLSLMFIFAGFLLLMIFDPGRNPVHLLFECVAAFSTTGLSIAGTMTLSTPSQVV
                     LMILMFVGRIGPVIVLSGFLATQTQNLYRLPVENIKMN"
     gene            10662..11591
                     /locus_tag="PLQ77_04215"
     CDS             10662..11591
                     /locus_tag="PLQ77_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09913.1"
                     /translation="MIRLLGNRNVIFILAIVLGLALPQPARWTVLAMMPALALVMTLA
                     TIHVPNSYFRHPRAILAPSLAGVAMTYGVLGSVILAASALFIRETSLWTGFLLIAAVP
                     PAVAVIPFTVILKGDLSWTLSGTVASYLAALLIMPLMFLIWIGTAFADPYKLIRIMLL
                     LIVLPLIVSRLILYWNWAGRIEPFRGLLTDWGFFIVLYSMIGVNRDLIFARPMMIAPA
                     ALVVLASTFGLGFVIEKAGVLLKKDPKRITSLVLLGTLKNQGIAGGLAIALFEKEAAL
                     PSAVYSVFMIVYVLWLDLRRRRTSSAPESHGGS"
     gene            complement(11588..12286)
                     /locus_tag="PLQ77_04220"
     CDS             complement(11588..12286)
                     /locus_tag="PLQ77_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09914.1"
                     /translation="MKYPLPAILFTLLILAPQAGAWHDKTHLAAARAGGLDSWYNAAG
                     PDLAKIKAGNVESYNHWFNNNAGAEVTAGMVMDQIERYNQRNKELDSEGHVYGAIIAS
                     LRAYDKDLRAGKYARYHLAYCAHYLTDLSQPLHNIAYDDFNQAFHDRNDGIVEAVILD
                     EPHLITRHMYRITLSDETFEADLAREIARIANLSRLLGYRLRAENRVMTKEEACVQLG
                     HSASLIRAVLRRYP"
     gene            complement(12313..13089)
                     /locus_tag="PLQ77_04225"
     CDS             complement(12313..13089)
                     /locus_tag="PLQ77_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL09915.1"
                     /translation="MNDMNPSKLLQRELMGISAVWWREYKVFWREKSRIVSSIVQPLI
                     WLFLFGSGIGASLSVENVHYRDYIYPGILTMSVIFGSVFFGLYIVWDRKLDVLKAVLV
                     APVARISIFFGKVLGGCTDVLLQVIILFAFSFLFPAVDLRGIPQALLILLVTSVALVS
                     LGLALGSLLESLEGFQVISAFLVFPLFFLSGALFPLDERLPSWLTGLVKINPLSYTVD
                     GVRGALLGMNKYPYIMDLGILLLFAAAMLIVGSILFSRMK"
     gene            complement(13086..14063)
                     /locus_tag="PLQ77_04230"
     CDS             complement(13086..14063)
                     /locus_tag="PLQ77_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL09916.1"
                     /translation="MAVIETRSLTKTFGKIQAVRGIDLSIEQGEIFGLLGPNGAGKTT
                     TIGMLCTIIRPTAGRGIVAGFDVAASPTQVRRNVGIVFQDPTLDTILTGRENLQLHAR
                     LYGIPAGMRALWIDEMLALVDLKDRADDLTRTYSGGMRRRLELARGLLHRPAVLFLDE
                     PTLGLDPQTRARVWEYIRQTAQKENTTVVLTTHYMEEAQQVCGRVGIIDRGKIIALDT
                     PQNMIDSLGGDIVAIRAESPPLDRIRSLPYVSSVRMVDGVMEATMKSAHLHLAQLLPL
                     IPGVQSVEMRKPTLNDVFMKLTGRDIRDDNAADSGSWAETIARYQQRGQ"
     gene            complement(14097..15476)
                     /locus_tag="PLQ77_04235"
     CDS             complement(14097..15476)
                     /locus_tag="PLQ77_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPL09917.1"
                     /translation="MEPSAMNALLQGQKAFFRSGKTRSISFRIASLKKLKAVISDHEK
                     EILAAVYDDLHKEATDAFATEISGVYTEIKLAIGNVSDWAARQKVPTPFFMRPSKSYI
                     YKEPYGVALIIAPWNYPFQLLILPLVGAIAAGNTAILKPSEISSHTAAILEKIIRAAF
                     PPEYICVVQGGAPETKALLELPVDYIFFTGSVPVGKAVMAAAARNLTPLTLELGGKSP
                     AIVHQTANLQTAAGRISWGKFLNAGQTCIAPDYVLAHESIRDAFLEELAKVIVKFYGR
                     DASTHPRYCRIINDRHFERLTALLDKTKVVCGGKTDAASRYIEPTVLSPADWDDPVMQ
                     EEIFGPLLPVLSYYDLDDAIRRINERPKPLALYLFANDKAVRRKIIRETSFGGGAINN
                     TIMHIVSHHLPFGGVGPSGLGRYHGQYSFETFSHAKSILRSYNRTDVADIARPYRGRM
                     MRLILKWMK"
     gene            15711..16499
                     /locus_tag="PLQ77_04240"
     CDS             15711..16499
                     /locus_tag="PLQ77_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPL09918.1"
                     /translation="MEGLTVERKGNVEVITLNRPEVMNALTYDMVQGLGDLFEAAQAD
                     DDIRALLLTGAGRAFSTGADLTGKGSARKDAHTPLGMRISTFGYSRMVNSIWALEKPV
                     VCAVNGTAAGASCNLALSCDLVLAADTAKFIQVFVRRGLIPDGGGTYFLPRLVGLPRA
                     KQLMLLGEDLPASRALEWGLIYNVVPADKLMDEAMEMAKRLAAGPTRAIGMIKSMLNR
                     SLESDLAAALEREASLQGIAAGTGDFIEGVMSFLEKRPPAFKGR"
     gene            16517..18208
                     /locus_tag="PLQ77_04245"
     CDS             16517..18208
                     /locus_tag="PLQ77_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09919.1"
                     /translation="MKKTDINSILKENESLKKRNRELENLLKKRVPGPVPVAQVQIYR
                     SLLHLCPASLVVSSLDDGVIYDVSDRFCRQSGFSRKEAIGRSSVELGLWVAPDEERER
                     FRDILLREGQCLNQEFRYRRKNGEVLTGMNFASLVTIDNRRYAIALVMDITARKQTEE
                     ALRESENRYRFLSEHMTDIAWIADVNLKTLYITPSLTKVLGFTPAERSRQRLDERMTP
                     DSVSRVREALARLLALEKKGKRIAEKPLTLLLEYYHRDGSTRWLETVVQGFHNTRGDL
                     TVIHGVSRDVTRRKQAQDELQRERIFLRSVIDGLPGVFYIADESDGLLFWNGNFEKTT
                     GFSEEEIRKMPVMGHIAEPDRRNLSNKIKQTALQGEISVEAGLVCKDGTVRDYLFQAN
                     RIEYQGRTCRMVAGTDITEHKQTQSALRSFAEELEDANKALRVLLRGRNEGQKAIEEK
                     LQANINDLVMPYLEKIGRAMREDPCKQYLNVLAGNLKEIVSPFMKNVQSLQKYLTPQE
                     IQVADLIRKGMRTKEIAGVLNTSDSTIGTHRNNIRKKLNLTRQGVNLRSYLQSLQ"
     gene            complement(18238..>19555)
                     /locus_tag="PLQ77_04250"
     CDS             complement(18238..>19555)
                     /locus_tag="PLQ77_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL09920.1"
                     /translation="AVAVRILTTKSNSGNKNPRGEKTMKIGKIILLGSMFVLALAAWT
                     SALSAEEVVIGYSGPLSGPAAEYSQDSINGIDMAIREINAAGGISVKGETFTFKLKRL
                     DDRAGMDQAIINARKFVNQDKAPVVFNSLATTIGALLKINEAKGGEFLLVGYSSVHTH
                     HNQGNRLLINPTPDFRSFVKAASDVAWQKGYRNGTMVVTLGEYGDAWRKHFRTVWEKK
                     GGKIIGDIPVNYYTETDFSAQLTSALATRPDFLLIGGPSATTALAVEQVRSMGFKGGF
                     VLTDQARMDYIADILKNRKLMEGMIGIANVADLPYPASAGFAAKYKETYKKAATWESA
                     LNYNTMHVLAKAMTIAGSARDPYAIRKAVGKALPLLGDKYPSELFSIEENGVMHSAGI
                     VQNVENGKFQKAYYTLDFPKTRAEFDKYKKVSRIREPENYRWVPVE"
BASE COUNT         4093 a         5406 c         5850 g         4206 t
ORIGIN      
        1 tggtgacgat ccagtgtcct aaatgccggt tttttgcaat ctgtggttga tacatcaaat
       61 ttatattcgt ataatgcgcc tgcgatggag gtgttttata cggaataaat taaccatctc
      121 aaatgcgtag tgtcggggaa atcgatgaaa cccattgtaa aaatgacgct ggcgatggcg
      181 gcggtgcttt tcttcacgtc ctgcgccgcc ggccacaacg cttgcctgaa aagcaggatg
      241 gatcgtcatg aagtccgctc gccggcgttt tggcaggact accggcgggg caacctcgaa
      301 gacaggcttc atgcggctcc cgaaccgctc ctggattatc tccatctgga taaccgtttg
      361 aacgggtatg acaccagacc tcaagcgacg gtcgcggatg attcgttccg gcaagatgtg
      421 cggcaggcca tggccgaact gcccgggccg gttcgggagg cggtgaaaga aaaactgatc
      481 ggaatctttt tcgttcgcga ccttggaacg acgggataca cggaggcggt tgccgatcct
      541 cagggaaggc ccgccgccgg tttcgttgtc ctggatgtcg gatcgcttcg acagaccgcc
      601 aatgcttggg ccacttccag agagaacacc gttttccaaa atgacggcga gggccggctt
      661 caggtcacgc tggaggaagc gccaaatgac aattccaaaa acgccatcca gttcattctg
      721 ctgcacgagt tcgggcacct ggtttccatc ggcgagcgct ttcatccgtc ctggctggac
      781 gaagcgaaac cggccggggc gatcgaagat taccttttct atcctctttc ctggcgcaag
      841 gaaaaagacg gctcgaatgt ttctctgttt gaggatgttt ttccggagcg tcgggatgtg
      901 cgcttttacg gagaggcccg cctgaaatcc ccgcagatgg cggatgttta ccgccgcctg
      961 gccatgacga atttcgtttc gctctacggg gcgaccggcc cttttgaaga ctttgccgag
     1021 tcgttcgcgc tgtatgtcca cagcgtctgg atgaaaaaac cctatcgcgt gcaaatcacg
     1081 caaagcggcc gtccggtttt tctctacgag tcctgctggg accagccgcg gtgcgccgcc
     1141 aaaagggccg ttctggaaga gtggttttcg cgttttcgca agccctgacc cgcgccgttt
     1201 gttcttgact atttcaactc aaactcgaag gtgctgacca cacgtatttt ttttacgggc
     1261 gtgctggtcg gactgtaggg cgcggattca tcgttcccct cgctgccgaa aatctggatc
     1321 atgccctgat tcgctgagcg aatttttccg atccgggcgc cggaatccga ggcaaactgc
     1381 tcggcggtga accgggcgtt tttggtggct tcggcaagca attgcggccg aagagcgttg
     1441 aaggttgtaa cgacgtatcg cggattggcc atgccttctc cctgctgtcc gtccagaagg
     1501 attcccgcct taagcagctc ttctgtggcc cccagcgccc gaatgaccat gtcgatgttt
     1561 ttggtcgtca cccgcagaga actggtgacc acgtagcgga accggtcgtt cttttcgttc
     1621 tggccgtatt cgcgggccag cttgtcaagc gtttttgagg gctgccaggt gacctcttca
     1681 tttttgaagc cctgtttttt gaggaaggca atggtggcgt cccggtcggc gatgattttg
     1741 gcgtgaacgt ctttcaggtc ttcgcccgcc ctgcggaagt tcagcgtcca gatggcctgg
     1801 tctgccttga cctctttttc caccagccct ttcaccgtga ccgtgcgggt gtccgacttg
     1861 aagcgggcga cgccttttcc gatgaaatat cccccgagga gaagccccgc cagcaggcag
     1921 acgccgacga tgacggcggc cggaaccaag gaacggtttt catgagtggt catagttttt
     1981 cctcctttta aaaggttatt ttttttccgc gggaaacgtc aaaccgcctc caaaaaatcc
     2041 aatgatggag accagcacag ccatcgaaag caggagaata aaatatatga cggcttccgt
     2101 cccgaaagcg gcgaaagctt ccggacgaag aattcgccag agaaacgcgg tggcggcgac
     2161 ggccagaagg ataaaggagg cgtatatttt aatgcggacg ggtttcatga actttgcctg
     2221 ataattgacc caccaggtca aaaaaccggt ggcgatggcc ggcacgatga aaagaatatc
     2281 cgccgccagg cagtgcaggg ccgtcgcctc gaacgaccgc tcgccggtca aaagatagag
     2341 aagatagaaa aacaccggcg ccatggagaa gaccatggga aaatgaacaa ccatcgggtg
     2401 cgggtggcgg cgcagaaaag gaacgcgctc catgagcgct tccagaggcg cgggcaaaaa
     2461 aggttttgcg ttcttctttt taaaaattcc aatctgcggg tatttgtcca gaacgtccgt
     2521 cccatggggc gcggccccga tggccagggt cagatcgctt ccggcctgat ggcgcttcat
     2581 gtgagaccct gttttccaga gcttgcttgc gctgacatcg atcaccctgt ctccatgcgc
     2641 aatgcagcag ggccttccgt cccggccgtc aaattgagcc agggattctt cgtcgaattc
     2701 tttcatggcg cctccttctt ctggaatctg attccgggcc ggccggcaaa gccgagggcc
     2761 ttttgccgcc ccacgggttg atttgaatat cctcggcgca ggcagggcag aaggctttgg
     2821 ggtccacatg gccgcatccg tagccccaaa ggtcaaattt tctgcactcc cggcacaggg
     2881 gcgcgtcgca gccgtcacaa tagacgactg cgtcattttt tccgcaaacg cggcagctcg
     2941 tttttgtttc gatgattctc agtgcaaggc tccgatgcgg ttctccgccg ctaaaattgg
     3001 taacgcaagc cggccgcaag tccatgggat ctcaggcggg actcgatgcc gttcacagcg
     3061 acggcggctt tataacacac ttttttcaaa acagacatcg ggaagatgcg attgcgcctt
     3121 gcgggggctg aaaattcata ttatgatgcc cacgtttttc agcggcggct cgccgacgga
     3181 aacctggaag aggggttgtt tatgggggac aggatgttca tcatgttcgt gcacctggtt
     3241 cagaacctcg cggtgctgat ggtgatggcc tatgttacga cccgcattcc ctggttcggc
     3301 gatttgctga atcggcggtt ctctttgaaa aacagtctgc ttctgggttt ggttttcggg
     3361 ctgatttcaa ttttcgggac gctcagcgcc gtgaagacgt tcggcgcgct ggccaacttc
     3421 cgcgatctgg ggccggcgat tgcgggcctg ctggccggtc cggtcgcagg gggagtggcc
     3481 ggcctgatcg gcggcatgca ccggtattct ctgggaggga taacgggcgt tccctgcagc
     3541 attgccacgg ttctggccgg actgatctgc ggcggttttt atctgtggaa aaagggccgt
     3601 ccgatccgga tccgggaggc aaccgccgtc atggcggtcg tcacgattct gcacggcttt
     3661 gtcattactc cgctcatact gggactttcg gaagagatcc ggcagattct gcggaccgtg
     3721 atgctgccca tgctgctggt caactgcgcc ggcatcgcgc tgtttttttt catccttcac
     3781 aacctcaacc gggagcgcct caacgaggcg gagaagcatc gcatcgacag cgaactgcgc
     3841 atcgcccgcg aaattcagat gggaatgctg cccgcaccgt ctcaaaacgg agatccccgc
     3901 tttgccgttc acgccgtttt gaagcccgcc aaagaggtgg gcggcgatct gtatcatttt
     3961 tttgcaaagg acaatgaccg gatttgtctc gccgtcggcg atgtgtccgg gaagggcatc
     4021 tcggcgtctt tgcacatggc catcacccag aagctcatca aggcgctggc gctctcgggt
     4081 gaggagcccg cggagcttct gggccggttg aaccgggagc tctgcgacgg caacgagtcg
     4141 atgatgttcg tgacgctctt tgtcgccttt atccatctgg aaagcggaaa gaccgtcttc
     4201 ggcaacggcg gacacaatcc tccctatcgg atccggcgcg gcgatgtttc gcgaatggtt
     4261 ctggagcccg gactggcgct gggcattcgg gaaaatgcgg tctataccaa tcagcggctt
     4321 tgtctggagg cgggagacac gctttttctc tataccgacg gggtgaccga agccatgaac
     4381 ggccgcaagg aacttttcgg cgaaaaacgt cttgcggcgg ctctttcgcg cctgcgggat
     4441 tctgatccgg aagggatctg ccgggggctc ctggaggagc tcgctcattt tgttggacaa
     4501 agcgaaccca gtgatgatat caccatgctc gccgtgcggc tgacggaaaa atcgaatgcg
     4561 gcggccgggg cggtccgcgg cggctgagcc ggcgcaggcg ccttcgcggg aagaggagcg
     4621 gttgatgaaa gtatggtcca aaagacaaca ggatgtagtg gttcttgccg ttcaggggcg
     4681 tctcgatgcg gcgacggcgc ctctttttga agagcaggtt ttgccgatga tttccgccgg
     4741 ggacaaaaat tttctgctgg atctggccgg tctggactac atcagttcgg cggcgctgcg
     4801 gcgtcttctg ttcttcgcga aacgggcgga agaccagggc ggcagggttg ttcttttatc
     4861 cgttcagaac cacattctcg agatcctgga catggccggc ttcacccagt tgttcgccat
     4921 tttcgccgac caggatgagg ccctccgttc cttttgagga aaacgcccat gccccggaaa
     4981 ccctccaatc tcatctatgg cgtcgatgaa aacccaggcc cgatcgcgct cgtcctgatg
     5041 gggcttcagc acatctccct gatgtccatc gcctttattt tccccgtcat tatcatcgaa
     5101 gccatcggcg ggaacctgga ggaagcagag gggctgattc gaatggccat gctggccacg
     5161 ggcatcggga cgatcctgca ggggatcaac cgggggccgg tcggttcggg ctatctgtgc
     5221 ccgcttctca acgggccggc cttcctgtcc gcttcgctga tggcgggcaa actgggcggc
     5281 ctttccctga tcttcggcat gacggcggtc gccggtgttt tcgaggcttt cttctcccgt
     5341 ttgctgtcgc gaatgcgggc cgtttttccc gccgaggtga cgggcaccat tgtgacgatg
     5401 gtggggatcg aagtcgttcc cctttccgtc cggaagtttc tgggaatcga cccgacgcat
     5461 tccatgccgg attcgacggc tgttctcatt gcgatgatca cgctgggggc catggcctgc
     5521 ttcaatgtct ggggcaaggg caaacttcgc ctgtattcgg tgctcctggg aatgatcgtc
     5581 ggatacgcgg cgtccgccat ggcggggctt ttgagccccg acaccttccg ggtgatgatg
     5641 gaagcccctt tctttgcgct gccggccgtc ggccattacg ggttttcttt tgagtgggtc
     5701 cttctggttc cttttctggt cgcggctctc tcgtcggctc tcaaaaccat gggggatgtg
     5761 accacctgcc agaaaatcaa cgatgcccag tggaagcgcc ccgacatggt caacatcagc
     5821 ggcggcgtcc tggcctgcgg catcagcaac attctgtccg gaatgctggg cgctctgggg
     5881 caatccgttt cgtcgagcaa tatcgggctt tccatcgcca cgggcgcaac cagccgccgc
     5941 atcgcctaca gcaccggaat catcctgatt cttctggcct tcttccccaa gtttgcctcg
     6001 atcttcgtca ttatgcccac gcccattatg ggcgcctcgc tgattttcgc cgtgagcttc
     6061 atgattctgg ccggtctgca gatcatcatg tcccgcatga tcgacgcgcg gaaaaccttt
     6121 gtcatcggga tttccatcat cttcggactc agcgtcgaat ttgcccccgg tctttaccgg
     6181 gatgtccacc cgtggatcaa accgatattc agctcttccc tgtcgctggc cacgatctgc
     6241 gcggtggtgc tgaatctgat cttccgcatc ggcatcgcga aaaccacctc cctggtcctg
     6301 acccccggcg agaatccgtc cgaagacatc gaccatttca tggaggagca gggccgcgcc
     6361 tggggcgccc gcagtgaagt catctacaag gcgaaggcgg cgatgattga attttacgaa
     6421 gccgccgccg aatccgctct gacgcgaaag cccgtcacga tgaccgcccg cttcgacgag
     6481 ttcaacctgg atgtggacat ggactacgag ggaaagccca tcgaactggc cgcctcaagg
     6541 cccgacgcga gggacctcct gacggatccg gacgccccgc tcaaactggc cggctacatg
     6601 attcgaagcc ttgcggatca tgccgcgatg gagtccgttg cgggccggaa ccgcgtgcat
     6661 ctccacttca accactgatt tcaccggttc ccgcgagccg tctcttcgcg ctttgctgaa
     6721 catcggatca acgacggaca tggaaagcgg aagaaagccg cggattcgtc gggtcagcgc
     6781 cggatcggtg tttccggggc ctccggtgtt ttgtctttgc tgaaaatcat attgccgaac
     6841 atcatcatca gaatcgtgag aaaaatgacc tgcgtgacga tgcccacgtt gatgaagggc
     6901 aaaagcagga tggagggaat gctgaggaac agcagaatgg tgatcagccc ccggggagcg
     6961 aaatacagca ggggattgag cggcaggcgc aggacgattc tgaagaaaag cgcccggaaa
     7021 ataaaaatgg acgcggtaat tgcagccgcg atcatcaggt tgttgaggtt gagcaggtcg
     7081 gacagccggg tgtaaaatcc gaacaggata aaaaagaagc agcggatgat aaaggtcagc
     7141 tcgaatacga tattcttaaa ggcgccgaga tccgcgttga attcatcgaa gttgatccat
     7201 tttgcggaga attcgttttt gaaaagaacg ttgttgttca tgaccaggcc gaagatcagg
     7261 acgacgagca gcgacggcag gtgaaccagc ttggccagcg cgtaaaccag aatgatcgcc
     7321 gtcatgatga tgacgtattt gacgtgatgg ctgatcttgt gcagcagaaa ggccaggccg
     7381 ccgctcagga ggatggatgc gatgaaggtc aggatcagct cggcgatgaa ccgaagcacg
     7441 cctgctccca gggaaccgct gctcaggagg atgaagtcaa acgcaaggat gccgatgatg
     7501 tcggagatgg agctttcgta agtgacaaat tcccggtctt ccggattcag actcgccgcc
     7561 gagggaatgg cgacggcgct gctgatcacg gcaaagggaa tgatgttgag cagggaggcc
     7621 tggaggtcgt agtggagaaa atacaccgcc acgtaagtga agacgccgat cgagacggca
     7681 aaaagcacga tggccgatga aacggagccg atgatgagac tgctcttgtc gcggcggatg
     7741 gccaggtcaa ggcttccttc cagaacgatc aggatgagtc cgagggtgcc catgatggga
     7801 agcattccgg acatgtccgg aatgccgatt ttgaaggaag atgccaggta attgatgaac
     7861 atgccggtcg cgatgagcag aatcacgccc ggaatcctgg tgtatttggc ggtgatgtcg
     7921 aagatgtatg ccaggagaat gatgacgcag aaggcgatga gaacgtaata ggtcatggcg
     7981 ttttgtcctc cggactaatc cacgaagcgt ctgatgtcgt tgagtctgcc gatgacgatc
     8041 agccggtctt tcgcgcccag cggacggtgc ggatcacccg acagatccac ttcgtactct
     8101 ttgccgagca gcgtggaaag gctcttttgg gccggtgcga ttttgacggc caccaaccgg
     8161 atgccgaagc ggttttcgag gttggccgct tccagcgtgt gaccgacgta tgcgtcaggc
     8221 accttcagtt cggcgatgat gattttatca ttgacaatca tggtgttgac ggcgttttgc
     8281 accatcagca tggcggtcag ctccgtggcg gtgtcttctt cgggatggat gatttcctcg
     8341 atgcccagct cgccgaggat gttctggtgc atgggattga tggcccggcc gatgatccgt
     8401 ttcactttct gttttttcag catggataga atcagcacgg aagaaccgat gtcctctccg
     8461 acggcgacga tgacggcgtc gacgtccgaa agcggcagag ccgaaagcgc ggcgggcttg
     8521 gtgcagtcca tgatcatgac gttggtgatt ttgtccttga gctcttcggc gcgctcgctc
     8581 cggcgatcga tgccgatgac ttcgtggccc agcgacgtca gtttggccgc gagcgacgaa
     8641 ccgaaaaatc ctaaaccgat gacgatgtat ctcattttgc ccttcaattc attttaatgt
     8701 tctccaccgg aagacggtag aggttttggg tttgggttgc aagaaagccc gataacacga
     8761 tgaccgggcc gatacggccg acaaacatca aaatcatcag aaccacctgg ctcggcgtgc
     8821 tgagcgtcat cgtaccggca atggacagtc cggtggtgga aaaagcggcc acgcattcaa
     8881 acagcaggtg cacgggattt cggcccggat caaaaatcat caacagcagg aacccggcga
     8941 aaataaacat cagcgacagc acaatgaccg aaaacgccct cacgatggtg gaggagccga
     9001 tttcccggtt gccgatttcc agccggtctt tcccccttaa aaaagacgcc accgttttca
     9061 ccgcgaccgc aagcgttgtg gtcttgatgc cgccccccgt cgatcccggg gaagcgccga
     9121 cccacatcag aaagatcgtc agaaacaccg tgggatacga ccaccctccc ggatccgtca
     9181 tgctgaagcc ggacgtacgc gcgttgacgc tcgaaacgag cgccgtcatc ccctgcgacc
     9241 agttgaagtg ctccggcagg gggctgcccg cctccaatgc ataatacaaa aacgctccca
     9301 gcaccaatag cacaagggtc gcatgcagcg caaggcgctc gccgacggcc agaagcagga
     9361 cttcgtgcgg gaggtttctg ccgaccaccc ggaaaaatat agattttacg ttgaacttga
     9421 ggactttgta gaaataaagc aaaagaggaa agccgattcc ccccagaacg accagcccgc
     9481 agaggatgaa ctgcagggaa aaattcgatt gaagatgcag cgcgttgaac tcctgcggat
     9541 tgaccgaaaa accggcgttg cagaaggcgg aaaccgcgtg aaagacggcg ttgtgggttt
     9601 tgtgcatcca gtcgccttcc agcgtgcggt aaatcaggac cgaacccgcc gcctccacca
     9661 gaatcgtgag cagcaggatt ttcagcagga gtttgtacag gccgcccaac tgatcgccgg
     9721 aaaagaaatc cttcagcaga aaccggtctt tcagggaggc ggagcccaga aagaggtaac
     9781 tgaaaaatcc cgtaaaggtc ataatgccca gcccgcctgt ctggatgagc cccagaatga
     9841 tgtttttccc catgggggaa aagactgcgg aggggtcaac aatcatcatg ccggtcacgc
     9901 ataccgcgct ggtggccgtg aaaagcgcct cgtaggcgga aatgggctga agcgtggcgt
     9961 tgggcagcat caaaagtccg gcgccggcga gaatcatggc cagaaaactt ccggcaaaaa
    10021 gaaaagaggg ggaaatattg atccgattca gggtgcccga caggtgatgc atttgcgtgg
    10081 ccatcagcgc gagcgatccc agcagaagag ctgtccggcc gcccagcgcg ccggaaatca
    10141 cggaccaggc ctccggaaat gcatgaaggt gcgcaagcaa aatcacaaag acaaccgcca
    10201 gaaccaggac gcggaacacc acggcgaacc attttgcggc gcgaaaggcc agcagttcga
    10261 tgagcagctt ggaggcaaac aatgccgccg cgagaagcag gtaggtctgg tggacggcca
    10321 ggctgtccgc cgcggttttt cggaagccga aatcaaaaag gatcaggctg ccaaagagca
    10381 gcgtggacag ggcaaagaca aagccagcga gcgcaataat cgccgatccc accgtgttcg
    10441 cccagtcgga cgcaacatag aattttttaa acagggcctc gttgtcgaaa tagctcatgg
    10501 ggtgaacggt ctttcttctt gcggttcttc gggggaaagt gtagcacagg tcctgaaaat
    10561 tatcagtgaa aaatcccggg ggttgggtaa cgtaatccgt cgtggcgcgc atccggagtg
    10621 attcaacgga aatctgaacg aatgaatttt tcaggaggct aatgattcga ctgctcggca
    10681 accgcaatgt aatctttatt ctggccatcg tactcggcct cgcgctgccg caacccgccc
    10741 ggtggacggt gctggcgatg atgcccgcgc tggcgctggt catgacgctg gccaccattc
    10801 atgttcccaa cagctatttc cgtcatcccc gggcgattct cgcgccttcc ctggccggcg
    10861 tggcaatgac gtatggcgtg ctggggagcg tcatcctggc cgcgtccgcg ctgtttattc
    10921 gcgagaccag cctatggacc ggtttcttgc tgattgccgc cgttccgccg gccgtcgcgg
    10981 tcattccctt taccgtcatt ctcaaaggcg atctttcctg gacgctgtcg gggacggtgg
    11041 cctcatatct ggcggccctg ctgatcatgc ccctgatgtt tctgatctgg atcgggacgg
    11101 cgtttgccga tccgtacaaa ctgatccgga tcatgctgct tttgatcgtt ttgcctctga
    11161 tcgtatcgag gctgattctg tattggaact gggccgggcg gatcgaaccg tttcgcggcc
    11221 tgctgacgga ttggggattt ttcatcgttc tgtattcgat gatcggcgta aaccgggatc
    11281 tgatctttgc gcggccgatg atgattgcgc cggccgcact ggtagtgctg gcctccacgt
    11341 tcggtctggg atttgtgatt gaaaaggcgg gcgttctcct gaagaaggat cccaaacgca
    11401 tcaccagcct ggtcctgctg ggcacgctca aaaatcaggg cattgccggc ggcctggcga
    11461 ttgcgctctt tgagaaggaa gcggcgctgc cttcggccgt gtacagcgtc ttcatgatcg
    11521 tttatgtcct gtggctggat ttgcgccggc gcagaacatc gtccgcgcct gaaagccacg
    11581 gcgggtctta agggtagcgc cgcaaaacgg cccggatgag cgacgcgctg tgccccagct
    11641 gcacgcaggc ttcctccttc gtcatgacgc ggttttccgc gcggagccga tacccaagga
    11701 ggcggctgag attggcgatg cgcgcgattt cacgcgccag gtccgcctca aacgtttcat
    11761 cgctcagggt gatccggtac atgtgccggg tgatgaggtg cggctcgtcc agaatgacgg
    11821 cctccacaat gccgtcattc cggtcatgaa aagcctgatt gaaatcgtca taggcgatat
    11881 tgtgcagcgg ctgggaaagg tcggtcaggt agtgcgcaca gtaggccaga tgataccggg
    11941 catacttgcc cgcccgcagg tctttgtcgt aggcgcgcag tgaagcgatg atcgcgccgt
    12001 acacgtggcc ttcggaatcc agctctttgt ttctctggtt gtaccgttcg atctgatcca
    12061 tcaccattcc ggcggtgact tccgccccgg cgttgttgtt gaaccagtga ttgtacgatt
    12121 cgacgttgcc ggccttgatc ttggccaggt ccggccccgc ggcattgtac caggagtcca
    12181 gccccccggc cctggccgcg gccaggtggg ttttgtcgtg ccaggccccg gcctgcgggg
    12241 ccaggatcag cagtgtgaaa agaattgccg gaagcggata tttcatgatg ctcctttcat
    12301 tttcgggaag ggtcatttca tccggctgaa caagatgctg ccgacgatga gcatggccgc
    12361 ggcgaagagg aggaggattc ccagatccat gatgtaggga tacttgttca ttcccagcag
    12421 cgctccgcgg acgccgtcca ccgtatagct cagcgggttg attttgacca ggccggtcag
    12481 ccaggagggc agcctctcat ccagcggaaa aagagcgccg gacagaaaga aaaggggaaa
    12541 caccagaaag gcggagatga cctgaaatcc ttccaggctt tccagaagcg agcccagggc
    12601 caggcccagc gacaccagcg cgacggatgt gaccagcaga atcaggagcg cctgtggaat
    12661 gccccgaaga tccacggcgg ggaacaggaa ggaaaaggca aagaggatga tcacctgcag
    12721 cagcacgtcg gtgcagccgc cgagaacttt tccgaaaaag atgctgatgc gcgccacggg
    12781 ggcgacgagg acggccttga gaacgtccag cttgcggtcc cagacaatat acagcccgaa
    12841 aaaaacagag ccgaaaatga ccgacatggt cagaatgccg gggtaaatat agtcgcggta
    12901 atggacgttt tccacggaga ggctcgcgcc gatgccgctg ccgaagagaa aaagccagat
    12961 cagcggctga acaatgctcg agacgatccg gcttttttcc cgccagaaga ccttgtattc
    13021 ccgccaccag acggcggaaa tgcccatcag ctctctttgc agaagtttgg aagggttcat
    13081 atcgttcatt gcccccgctg ttgatatctc gcgatggtct ccgcccagct tccgctgtct
    13141 gccgcgttgt cgtcgcggat atcgcggccg gtgagcttca tgaagacatc gtttaaggtc
    13201 ggttttctca tttccacgct ttgcactccg gggatcagag gcagcagttg tgcaagatgc
    13261 aggtgcgccg atttcatggt cgcttccatg acgccgtcca ccatcctgac cgaggataca
    13321 taggggagcg accggatgcg gtccagcgga ggcgattccg cgcggatcgc tacgatgtct
    13381 cctcccaggg aatcgatcat gttttgcggc gtgtccagag cgatgatttt tccccggtca
    13441 atgatgccga cgcggccgca cacctgctgg gcttcttcca tgtagtgtgt ggtgaggacc
    13501 acggtggtgt tttctttttg cgccgtctgg cggatgtatt cccagacgcg ggcgcgcgtc
    13561 tgcgggtcca ggcccagcgt cggttcgtcc agaaacaaca cggccgggcg gtgcagaagg
    13621 ccgcgcgcca gctcgagcct tcgccgcatg ccgccggaat aggtccgggt cagatcgtcg
    13681 gcccggtcct tcagatcgac cagcgcaagc atttcatcaa tccagagggc gcgcatgccg
    13741 gccggaatcc cgtaaaggcg cgcgtgaagc tgaagatttt cccgaccggt gagaatcgtg
    13801 tccagcgttg ggtcctgaaa aacaatgccg acgtttcgcc ggacctgcgt cggcgacgcc
    13861 gcgacatcga agcccgcgac gatgccgcgg ccggcggtgg ggcggatgat ggtgcagagc
    13921 attccgatgg tggtggtttt gccggcgccg ttcgggccca gcagtccgaa gatttctcct
    13981 tgctcaatcg acagatcgat gccgcggaca gcctggattt tgccgaaggt ttttgtcagg
    14041 gatcgggttt cgatgacggc catatcgtta ggattccgga gatttttctt tgcgcatcat
    14101 ttcatccatt tcagaatcag ccgcatcatt cttccccggt aaggcctggc gatatccgcg
    14161 acatccgtcc ggttgtagct gcgcaggatg cttttggcgt gggaaaacgt ttcaaagctg
    14221 tattggccgt gataccggcc cagaccgctg ggacccacgc cgccgaacgg caggtgatgg
    14281 gacacgatgt gcatgatggt gttgttgatg gcgccgccgc cgaacgaggt ttcccgaatg
    14341 atctttctcc ggacggcctt gtcgttggca aacagataca gggcaagcgg cttgggcctt
    14401 tcattgattc ggcggatcgc gtcgtccaga tcgtaatagg aaaggaccgg aagcagcggg
    14461 ccgaagattt cctcctgcat gacggggtca tcccagtccg ccggactcag cacggtgggc
    14521 tcgatgtagc gcgaggcggc gtcggttttg ccgccgcaca ccaccttggt tttgtccaga
    14581 agcgccgtca gtcgttcaaa gtgccggtcg ttgatgatcc ggcagtagcg cggatgcgtt
    14641 gaggcgtccc ggccgtaaaa tttcacgatc actttcgcca gttcctccaa aaaggcgtcc
    14701 cgaatggatt cgtgcgccag cacataatcc ggcgcgatgc aggtctgccc cgcattcaga
    14761 aattttcccc aactgatccg gccggccgcc gtttgaaggt tggccgtctg atgaacaatg
    14821 gcggggcttt tgccgcccag ctccagcgtc agcggggtca ggtttctggc cgccgccgcc
    14881 atgaccgcct tcccgacggg aacgctgccc gtgaaaaaga tataatcgac gggcaattcc
    14941 agaagcgcct tcgtttcggg cgctcccccc tgaacgacgc aaatgtattc cgggggaaac
    15001 gcggcgcgga tgattttttc caggatggcc gccgtgtgcg acgaaatttc cgaaggtttc
    15061 aggatggccg tattgcccgc ggcgatcgcg ccgaccagcg gcaggatcaa aagctgaaaa
    15121 ggataattcc agggcgcgat gatcagcgcc acgccgtagg gctctttgta gatatagctt
    15181 ttggacggtc tcataaaaaa cggggtcgga actttttgcc gcgccgccca gtcgctcacg
    15241 tttccaatcg ccagcttgat ttcggtgtac acgccggaaa tttccgtcgc gaaggcgtcg
    15301 gtggcctcct tgtgcagatc gtcatacacg gcggccagaa tttccttttc atgatcgctg
    15361 atgacggcct tgagcttttt cagcgaagca atgcgaaaag aaatgcttct cgtttttccc
    15421 gatcggaaaa aagccttttg tccctgcagc agcgcgttca tcgcggatgg ttccattcat
    15481 gcgcctccag aaggtaatgc ggaagattct aaaaacagcc cgccgtccat gtgcgtcaac
    15541 tataagcgca tggcgccggc gtgtcaagcg aaagccccct attcctcact catcgctcgt
    15601 tgcttcttca tcgccagttt aacattgaca tccggcggtg ttttgcctac agtccgcatg
    15661 agaaaacagc aaaacgcttt tccataaaag ccaacggagg aaagtgaacc atggagggac
    15721 tgaccgtcga acgcaaggga aatgttgagg tcatcacact gaaccgcccg gaggtcatga
    15781 acgcgctcac gtacgacatg gtgcaggggc tgggagatct ctttgaggcg gcgcaggccg
    15841 acgacgatat ccgcgcgctt ctcctgaccg gcgccggacg ggcgttttcc accggcgccg
    15901 acctcaccgg caaaggcagc gccagaaaag acgcccatac gcctctgggc atgcgcatct
    15961 ccacgttcgg ctactcgcgg atggtgaact ccatctgggc gttggaaaag ccggtggtat
    16021 gcgccgtcaa cggcactgcg gcgggcgcca gctgcaatct ggccctgtcc tgcgatttgg
    16081 tgctggccgc cgacacggca aagttcattc aggtgtttgt gcggcggggg ctgatccccg
    16141 atggaggcgg cacctacttt ttgccccggc tggtgggtct tccccgagcg aagcagctca
    16201 tgctgctggg cgaggatttg cccgcttcca gggcgctgga atgggggctg atctataacg
    16261 tggtgcctgc ggacaaactg atggatgagg ccatggagat ggcgaagcgg cttgccgcgg
    16321 gtcccacccg ggccatcggc atgatcaaaa gcatgctgaa ccgttcgctg gaatccgacc
    16381 tcgcggcggc gctggagcgc gaggcctcgc tgcaggggat cgccgccgga accggcgact
    16441 tcatcgaggg agtcatgtct tttctggaga agcgtccgcc cgcattcaag ggccgctgac
    16501 ttccggcggt tgccatatga aaaaaacgga catcaattcg atcctgaagg aaaacgaatc
    16561 cctcaaaaaa agaaatcggg agctggagaa tcttctaaag aagagggtgc cgggcccggt
    16621 tcctgttgcg caggtacaaa tctaccgatc cctgcttcac ctgtgccccg cctccctggt
    16681 cgtgagcagc ctggacgacg gcgtcatcta tgatgtcagc gaccggtttt gccggcagtc
    16741 cggtttttca cgcaaagaag ccatcggccg gtcatccgtg gagctgggat tgtgggtggc
    16801 gcccgatgag gagcgggaaa gatttcggga cattctcctg cgcgagggcc agtgcctgaa
    16861 tcaggagttc cggtatcgaa gaaaaaacgg agaggtgctc accggcatga atttcgcgtc
    16921 gctggttacg atagacaacc ggcgctacgc catcgccctg gtaatggaca tcacggcccg
    16981 gaaacaaacc gaggaagccc tgcgggaaag cgagaaccgg taccggttcc tttcggagca
    17041 catgaccgat atcgcgtgga ttgcggatgt gaacctgaaa accctctaca ttaccccgtc
    17101 gctcactaaa gtgctgggtt ttactcccgc ggagagaagc cgtcagcgcc tggacgagag
    17161 aatgacgccg gattccgtat cccgcgtgcg ggaggctctg gccagactgc tcgctcttga
    17221 aaaaaaggga aagcgaattg ccgaaaagcc gctgaccctt ctgctggaat attatcacag
    17281 ggacggctcg acccgctggc tggaaacggt cgtacagggc tttcacaata cccgcggcga
    17341 tttgacggtg attcacggcg tgtcccggga tgtgaccagg cgcaagcagg cgcaggacga
    17401 attgcagcgg gagcggattt tccttcggtc ggtgatcgac ggcctgccgg gcgtatttta
    17461 catcgcggat gagtcggatg gacttttgtt ctggaacggc aacttcgaga aaacaaccgg
    17521 tttttcggaa gaggaaatca ggaagatgcc ggtgatgggc cacattgccg aaccggaccg
    17581 gcgaaatctg tcgaataaaa taaagcaaac agccctgcag ggagaaattt ccgtggaagc
    17641 cggtctcgtc tgcaaggacg gaaccgtcag ggattatttg tttcaggcaa accggatcga
    17701 atatcagggg cggacctgcc ggatggtcgc cggcaccgac attaccgaac ataagcagac
    17761 ccagagcgcc ctcaggagct tcgccgaaga actggaagac gccaacaagg cgctgcgcgt
    17821 attgctgcgc ggccggaacg aagggcaaaa ggcaattgaa gagaagctgc aggccaacat
    17881 caacgatttg gtcatgccgt atttagaaaa gatcggaagg gcgatgcggg aagatccgtg
    17941 caaacaatac ctgaatgttc tggccggcaa tctgaaagag attgtgtctc ccttcatgaa
    18001 aaatgtgcag tctttgcaga agtacctgac cccccaggaa attcaggtcg ccgatctgat
    18061 tcgcaagggc atgaggacca aggaaatcgc cggtgtgctc aacacgtcgg acagcacgat
    18121 cggaacgcac cggaacaata tccggaaaaa actgaacctg acccgccagg gcgtcaatct
    18181 tcgttcctac ctgcagtcgc ttcagtagcg ctcccggcaa aacggccggc tgcggggtta
    18241 ctcgacggga acccaccggt agttttccgg ttccctgatc ctgctcacct ttttgtactt
    18301 gtcgaattcc gccctggtct ttggaaagtc cagcgtgtaa taggcctttt gaaattttcc
    18361 gttttccaca ttctggacga tgccggcgga atgcatgacg ccgttttcct cgatgctgaa
    18421 cagttcgctg gggtacttgt cgcccagaag aggcagcgcc ttgcccacgg cctttcgaat
    18481 ggcgtaagga tcacgggccg agccggcgat ggtcatggct ttggccagga catgcatcgt
    18541 gttgtagttc agcgcggatt cccacgtcgc cgcttttttg taggtttctt tgtattttgc
    18601 ggcaaaaccc gcggaagcgg ggtagggaag atccgcgacg ttggcgatgc cgatcatgcc
    18661 ttccatcagc ttcctgtttt tgaggatgtc cgcgatataa tccatgcggg cctgatccgt
    18721 caggacaaaa ccgcctttga accccatgct gcgcacctgc tcgacggcca gggcggtcgt
    18781 cgccgaaggg ccgccgatga gaagaaagtc cggccgggtc gcgagcgcgg aggtcaactg
    18841 cgcggaaaaa tccgtttccg tgtagtagtt gacaggaatg tcgccgatga tcttgccgcc
    18901 ttttttctcc cagaccgtcc ggaaatgttt gcgccaggcg tcgccgtatt cgcccagggt
    18961 cacgaccatc gttccgttgc ggtacccctt ttgccaggca acgtcggagg cggccttgac
    19021 aaaggagcga aagtccggcg tgggattgat gagcagccgg tttccctggt tgtggtgcgt
    19081 gtgcacgctg gaatagccca ccagcagaaa ttcaccgcct ttggcttcat tgatcttcaa
    19141 aagggcgccg atggtcgtgg ccagcgagtt gaacaccacg ggggccttgt cctggttcac
    19201 gaatttgcgg gcgttgatga tggcctggtc catgcccgcg cggtcgtcca gccgtttgag
    19261 cttgaaggtg aaggtttccc ctttgacgga gattcccccc gctgcgttga tttcccgaat
    19321 ggccatgtcg atgccgttga tgctgtcctg gctgtattct gcggcgggtc cgctcagagg
    19381 gccgctgtat ccgatgacca cttcttccgc ggacaacgcc gatgtccagg ccgccaatgc
    19441 cagaacaaac atcgaaccta acaagattat tttgccgatc ttcatggttt tttcgcctct
    19501 cggattttta ttgccgctgt ttgattttgt ggtcaaaatc ctgaccgcca cggcc
//
LOCUS       DAOPAF010000026        19346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_2939_length_19346_cov_75.412109, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000026 DAOPAF010000000
VERSION     DAOPAF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19346
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_19346_cov_75.412109"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PLQ77_04255"
     CDS             complement(<1..1460)
                     /locus_tag="PLQ77_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09921.1"
                     /translation="MNDHAGTRQALLGELASLREKIAKLERSALDQKRVKEALRESEE
                     KYRLLFEYSPLGNFYFDEKGVIVACNDHFVDIIGSSKEVLIGLDLFALRDQKIVDSIR
                     QVLEGQTVFYEDVYHSVTAKKSTPVRVIFTPVHLEGKGIIGGVAMIEDITERRRAEAA
                     LRESEEHYRSLVENADEAIIVIQDGRIRFVNARAVEAFGYSVQEFMSIPVFELVHPED
                     RDMVIRRYLEKINGDAAPTRHTYRALYKKGLVAWIEISSILINWEGRPATLNLITDIT
                     DRKKSEDALREREEKYRTILESIEDGYFEQDLYGNFIFVNDSMSRIYGYSKEELIGLN
                     YKQYTDFESGRKCFHIYLNIYKTGKPGKVVDFDIVRKDNTRRHVESSVSLIRDPTGKP
                     IAFRGIVRDVTERKQSEEALSQSFVKLRAALAATVQATAMMVETRDPYTAGHQRRVAD
                     MGRAIALEMQLTDEQIEGIHMAGMVHDLGKISVPSE"
     gene            complement(1624..2934)
                     /locus_tag="PLQ77_04260"
     CDS             complement(1624..2934)
                     /locus_tag="PLQ77_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPL09922.1"
                     /translation="MRANSIQPVTPDEAVRAVRSGDHVHISATSNTPACLVEALCRRG
                     RSGELRGVRIHHLHTEGPAPYADPAFEGVFQLDSFFVGPNARRSTQQGYADYIPVFLG
                     ETQRLYREGYLPCDVALIQVSPPDERGRVSLGTSVDASVAAVEKARVVIAVINPHVPH
                     TYGDGLIHASRIHFFVEDDAPLETVHFALPDAVEAAIGKHCASLIEDGATLQMGIGAI
                     PNAVLTQLSGHKNLGIHTEMFADGVIDLVEKGVINGENKAIDRGQMVATFLLGSKRLY
                     DYTHCNDRVLMKDVGYTNDPFIIAKNPKATAINSALQVDLTGQISADSLGTKCYSGVG
                     GQIDFFYGASRSAGGKPILAMPSVTEKGISKIAPILLPGAGVVTTRAHAHWLVTEFGA
                     VNLYGRSLQERARLIIGIAHPDHREALDRAAFERFGPHYRFAPA"
     gene            complement(3045..3845)
                     /locus_tag="PLQ77_04265"
     CDS             complement(3045..3845)
                     /locus_tag="PLQ77_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL09923.1"
                     /translation="MPHQTVNDIKIYYEEKGTGEPLLLLDGLAFPMDLWFAQIEELSK
                     DFRVIAMDNRGIGRSDKPDDVYSMELMASDSVGLLRALGIQKAHVAGLSMGGFISQEI
                     ALSYPEMVNRLILVATGMGGHRSLTLGKPFWDRTAAAIAGKKPWEIYRIDLTLMTAPG
                     FVEQQPDTLDKAVELRMKNPQPFHAFVRQFTAAGLFDSNERVHKIDQPTLIVLGREDP
                     IFPIALAEDFRQKMPQAKMVIYENCGHAIHLEKPDRLSNDIREFLKGN"
     gene            complement(3926..5215)
                     /locus_tag="PLQ77_04270"
     CDS             complement(3926..5215)
                     /locus_tag="PLQ77_04270"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPL09924.1"
                     /translation="MSNAVIVSGVRTAVGAFGGSLKDVPAKDLGALVIRESLIKAGFK
                     PVLPAHAKEDAPDTAKNEGLCPVEQQYSRWDANLREIAVDEVIMGNVIGAGQGQNVGR
                     QAMIRAGLPKETTAITVNKVCASGMKAIALAAATIKAGDNEVVIAGGMENMSDVPIGL
                     PKARWGHRMDMPFGKTVDLMVFDALYEIFYGYHMGVTAENIAARYNISREEQDKLSLE
                     SHTRARNAIKSGLLKDEIVPVTIPQKKGNPIVFDTDERPMETSMEKLAKLGTAFKKDG
                     TVTAGNASGINDAAVAMLIMSEDKAKALGVKPLARIKGYASGGVDPAYMGLGPVPAVR
                     KLLRITGTSVKDYGLFELNEAFASQAISCLRELNIPWDITNVNGSAISIGHPVGCSGA
                     RIVLALANEMKRRNVGLGLASLCIGGGQGMAIAIEAV"
     gene            complement(5374..7272)
                     /locus_tag="PLQ77_04275"
     CDS             complement(5374..7272)
                     /locus_tag="PLQ77_04275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL09925.1"
                     /translation="MKDCTMAGFFDYRSKVIGTDQVLEKLKPGQKIFLSSGVAAPGKV
                     LTALARSDAKNLRDLEIIQLITLRKYLSHQSSDQYRLKTFNVGESISKEVAEGRVDFI
                     PANLMELPYIFLSGAVAVDVAIIQTSVPDDKGFMSLGVAVDVANIVIKQAALVIAEIN
                     PNMPVTLGETTIHISSIDYVVESDLPLVERINRPYDEITDRIGWNISNLIDDGATVVL
                     HVGRLFNAVADHLKQKRNLGIVTHVVSDWAVDLIESGAISLDRSRFNGGLITASYCYG
                     SKALYRYVHRNPIFEFYPIARLNNPFVVRRIKSLIGIMNVKKIDISGESVIFHSGDNL
                     LTGYESKLNFAVGATMTRRGKAIVALNSVDTEGNSNIVIKFDGRTDNTHSTLGVVKYI
                     VTEYGVANLFGKSIRERALAMIDIAHPDHREDLLRQAKALNYVYADQIYVARHAANYP
                     ALLETRKSLKDSLEIKVRPIKPSDEDKMRRLFYNFSDESKYFRYFAPKPVMPHKEMQT
                     YVSIDYDKVLSVVAVAEKGRNERIIAEARYAYDKSAGAYEIAFIVDEDFQGRGVATFL
                     FKYLIKIARDRGIQWFIAYVLPRNESMMKVFEKSKLPIQKSYENDALVLRFDLTQEVA
                     DTDAAARK"
     gene            7428..8876
                     /locus_tag="PLQ77_04280"
     CDS             7428..8876
                     /locus_tag="PLQ77_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPL09926.1"
                     /translation="MKKIRKVLVANRGEIALRIIRTVKEMDLDTVAVYEKPDREAYFL
                     RFADQAILMGDGPRRDYLNIDKIIWAAQKSGSDAIHPGYGFLSENPDLAQACVDAGIS
                     FIGPPPQVIRDLGNKVVARNIMEKAAIPFVPGTGTLSAGDAGLTEAVAFGKTHGYPIM
                     LKALAGGGGRGIRKIDSEEELRSQLPQARSEARSAFNDDRIYIEKCVESPRHVEVQIL
                     ADAYGHVMHLGTRDCSIQRRHQKLLEIAPADLPKNVLDAMHQTAIRAAREAGYVNAGT
                     VEFLVDPKTNQFWFMEVNTRLQVEHTVTEILTGIDIIRQQVLLAEGGRLEIPEERIHL
                     VNKAIQVRINAEDPKNNFMPEGGKRVEVYQSPGGPEIRLDGIVYQGYKIPTDYDSLLV
                     KLTVRGYSWQQTIQRLKRALKDFLIVGPKTTIPFYLSICDDADFRDGRFTTDYLDAHP
                     ELFDYPEPEREIARLSKLIAEIHSRKINPYAY"
     gene            8906..10834
                     /locus_tag="PLQ77_04285"
     CDS             8906..10834
                     /locus_tag="PLQ77_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HPL09927.1"
                     /translation="MKILPEERITPRELQRAGVKAVLEKMRGQKGYYLTNTERDLSQS
                     DFKNRIMPHTQILVAPERNAAGYFSLEITGGASVHVDMLRKQMNPLEKLEVLAVHMPD
                     TLFQTLCRGINLFGYRPYPENVIRLTVETFARHVHVWRVFDFLNYIPNMVPVYEEVQK
                     AGRMLEAAVCFSTGPEHTNRFYVKKVGEILDVTGPDILLAIKNHGGLGSPGRIGELVR
                     AILQKYPDLLIHYHGHNTDGNEIGRIVAAVQNGAKIVDASDHALTGFYGPPPLLTVVN
                     TLEDYGYPAVGLDKQAVIDASSKLRHERPCYRDFESQFFGFDPTVQTHKLPGGATGSS
                     FEQAQKGGFLDRMPEILQNELPRVQIELGNFWSVTPGSQILWTTAANNVVKGARYRDV
                     SDDLKNLLLERYGKFPFYKPAPEIYEAVFGKDWKKIVRREAGYQKIEDVDLDVERRIL
                     EHAIGRKATDDELVLYLQHPNDAVGFFKFEEKFGKTWVLPPKVWFRRGGFAIGDKFEF
                     PDATGKLHTIEIGPSRRTKDGSHMTHLGVDYHPEPIVTPAEEEGEKPKAALALSAKEI
                     GALARMGDIRATLKGVVNQIPVAVGDEVGAGDTLVVLEAMKMLTNIASDVDGKVSEIF
                     SSPGSSVETGDKLMLIEI"
     gene            complement(10831..11622)
                     /locus_tag="PLQ77_04290"
     CDS             complement(10831..11622)
                     /locus_tag="PLQ77_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPL09928.1"
                     /translation="MKIKKVANQTRIRIGLFGFGKTGRLVAEEFFQDGQFDLEWVVRK
                     THKEHHKYASRLLGYEVDDARVFSVDESTPDFYRRHPVDIIVDFSSAAAHRQYAPAAD
                     FGIRIISAISKYRAEDLEDLKRMSEKTAVLYSPNITLGINFLLVASQILQGIAPHADI
                     EIVEEHFRGKKEESGTALKIAEILGLEKERRVNSIRVGGIVGKHEIIFGLPNQTIRLV
                     HESISRAAFGQGAIFAAKWLIHAPPGLYSMENIIVDMFRKNIPVY"
     gene            complement(11699..12109)
                     /locus_tag="PLQ77_04295"
     CDS             complement(11699..12109)
                     /locus_tag="PLQ77_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPL09929.1"
                     /translation="MRDFKNILYAMDLDGEHFSSMVHALEFARRFTSRIHILYVNDPQ
                     AGYRHPADHEDAVALKVKEAAPEFLLENLDVVYAVSKGDTAGEIVQYARENLIDLIIV
                     GHKHRGKLYASMFDSTDVHVIDEAMLPILVFPEM"
     gene            complement(12239..13105)
                     /locus_tag="PLQ77_04300"
     CDS             complement(12239..13105)
                     /locus_tag="PLQ77_04300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL09930.1"
                     /translation="MSRVSAVLLLMLSLAAMPAESGSSPDVSFSSDAMVRGGLILLSI
                     QGCESDDLQVVWMEKEIPLLRGRDDGAWRGFIAADLNQKPGVYPVAVRVPSLEMERTF
                     EIQVNDRDYGVRKIRLPRAKVDLDEASLKRVKTEARAVSALWKAECRPPEWEGSFWMP
                     VNNKIVGPFGQRSIINRQERSLHTGVDIRGKTGSPVRATNAGTVVLAADHFFTGKSVF
                     VDHGSCIFSMYFHLDQIRVEEGEQVEKGRILGSVGATGRATGPHLHWGVRISGARVNP
                     LTLIELSRDLES"
     gene            13465..14049
                     /locus_tag="PLQ77_04305"
     CDS             13465..14049
                     /locus_tag="PLQ77_04305"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPL09931.1"
                     /translation="MDIAAFYHSEIFKFVLLPVLIFLARICDVTLDTLRIIYVSRGMK
                     FLAPAIGFVEVLIWLVAITQIFKNLSNPICYVAYAGGFAMGNFIGIIIEEKMAIGTVV
                     IRIITQKDAEALIGCLKENRYGVTHVDAQGAMGQVKIIFTIVKRKSIDSVLEIVRGCN
                     PLAFYTIEDVRSVRKGVFPLFKAAGKGPGPAADG"
     gene            complement(14136..15884)
                     /locus_tag="PLQ77_04310"
     CDS             complement(14136..15884)
                     /locus_tag="PLQ77_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPL09932.1"
                     /translation="MNVAVVLIIAVVLFFLAYRFYARFIAKLFDENDNRPTPACALRD
                     DRDYVPTKPVVLFGHHFAGIAGGGPIIGPTVALLFGYLPVWLWAVIGTIFIGAVQDMT
                     VLFASVREKGKSIAEIARESLGNTGFFLFISFAILMLLLLTSAFLGLTAVSLTSLVPL
                     EMMRLTPDQTIVKTVLVGGVPNAQIGGISSTSLIIITAFAPVVGWLVYKRGVSVKLAV
                     PLAVIVCIGSILYGMKYPVSLDPDVWMVILCFYTLLAAGIPVWIILQPRDFTNSFLLY
                     IGVAALFAGGIAAGFQGLTFTAPAFNLAQGADKLGPVWPFLFITVACGAVSGFHALVA
                     GGTSSKQVSRETHVKKIAFGGMLLEGLLAIGVMIAVGCGIAFADFVNIAFPSAAGVKA
                     NPILAFALGVGGLLDKSLGIPPVYGTVFGILMVEGFVVTTLDTAVRLNRYLFEELWQV
                     VFRKVPKIMGSYLFNAFLCVSLMYFLAYTNAFAAIWPIFGSANQLLASLALIAVSAWL
                     VKRKKTAWFTVLPAVFMMITTIYSLVSLLVNKYLPKHNLALAVTDVALILLSIGVIVL
                     ALKSWRDLKAKAGQAA"
     gene            16169..16951
                     /locus_tag="PLQ77_04315"
     CDS             16169..16951
                     /locus_tag="PLQ77_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPL09933.1"
                     /translation="MAFEYEKLVVEQDGAVVVAAINHPPANAMAQGVLRDLTDLLNRC
                     EKDDSVRVLIITGTGPKCFSAGADITEFADLQSGKVPQYDGNDVYFKIENYPKIIIAA
                     IQGLALGGGLELACSCHLRIMAEEAVVGLPEVKLGLNPGWGGTQRLPRYIGKTKALEL
                     MLTGDFMPAKEALALGLLNKVVPAAEVLETAKKLAKKLAGGAPVAQREIMKAVRLGLE
                     GTLYDGITRVEKAAFQTLLVTEDFKEGAKAFLEKRPAQFKGR"
     gene            complement(17010..17627)
                     /locus_tag="PLQ77_04320"
     CDS             complement(17010..17627)
                     /locus_tag="PLQ77_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09934.1"
                     /translation="MPKNTILHALVFVQNWGEEDGLMTFLRGNPHAVSIFHVMGRHSY
                     LIDSNFDDREQLHDWINRLKALRLSSGVPAVISVQTQKVIQVFKQKNDFDLKDYKDLT
                     DRFHFFMLIDNPHHDEKLIELLKKMRIVSSILHVQGANSFIVEIITESYDSYKKMLGK
                     AKELKSIQHIETLEVLSVKKYRNQVIDKGGELSYPQADLRELYTL"
     gene            complement(17872..19233)
                     /locus_tag="PLQ77_04325"
     CDS             complement(17872..19233)
                     /locus_tag="PLQ77_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL09935.1"
                     /translation="MMKRASRVKWGVVTALAGLVAMAISLPSVAVAQPKGEPFVIGYI
                     GMVGSPGTRPCMDIQKMAAEEINATGGIMGRPVKYVVMDNKGDTSLSVEAFRKLVIED
                     KAKFISVEGRTEICLAVQEASGMLFKEYPHILIFNGPMGAELTARIIDEWPKYNHCFR
                     DWDPEPAHYAQTKYFFCKTMPQYQKTKRIAFLWEDLAWTTEWRKGISYINLPTWEKMA
                     KDCGLEVVYSKAVKPRGTMYLPILQDIKKSNADLIFYISSWFTDTESFAKQWSDSAAR
                     DIQVNLYGGVAQTQDYWKMTGGKGLGVISSYTDLDTIPLTPKTIPLVKKAHARKIPMQ
                     IHVHIAYADIYHFKSAIERAKGTGNIKTLIKAMEDVETVYSLGKMKYETRKIKPFYHS
                     RVRVDAANPWKTYPGYFLQLIVQFQEGGKIAFLHESCEENEKLVSKWVDPKRYKTPAE
                     LRK"
BASE COUNT         4210 a         5605 c         5378 g         4153 t
ORIGIN      
        1 atctcggagg gaacggagat tttgcccaga tcatggacca tgcccgccat atgaatgcct
       61 tcgatctgct cgtcggtcag ttgcatctcc aaggcaatgg cccggcccat gtcggccacc
      121 cggcgctgat ggcccgccgt gtagggatcc ctggtttcca ccatcatggc cgttgcctgc
      181 acggtggcgg caagcgcggc gcgaagtttg acaaagcttt ggctcagggc ctcctctgac
      241 tgcttgcgtt ccgtgacgtc ccgcacaatc ccccgaaagg cgatcggttt tcccgtcgga
      301 tcacggatga gagaaacgga cgattcgaca tgtcttctgg tgttgtcttt ccggacaata
      361 tcaaagtcaa cgactttgcc gggcttcccg gttttgtaaa tattcaaata gatgtgaaag
      421 cattttcttc cgctttcaaa atcggtgtac tgcttgtagt tcaatccgat taattcttcc
      481 ttcgaatacc cgtagattct ggacatggaa tcgttaacga atataaaatt gccgtataga
      541 tcctgctcga agtagccgtc ctcgatgctt tccagaatgg tccggtactt ctcctccctt
      601 tcccgcaggg cgtcctccga ttttttgcgg tcggtgatat ccgtgatgag attcaacgtc
      661 gcgggacggc cctcccagtt gatgaggatg gagctgatct cgatccacgc gacgaggcct
      721 ttcttgtaga gcgcccggta ggtatgccgc gtgggggcgg catcgccgtt gattttctca
      781 agataccgcc ggatcaccat gtccctgtcc tccggatgca ccagttcaaa gacagggatc
      841 gacataaatt cctgcactga atatccaaag gcctcgaccg cccgggcgtt gacaaacctg
      901 atcctgccgt cctgaatcac gatgatcgct tcatcggcat tttcgaccag agaccggtaa
      961 tgctcttcgc tctcccgcag cgccgcctcc gcgcgcctgc gttcggtgat gtcttcaatc
     1021 atcgccacgc cgccgataat gcccttgccc tccagatgga ccggcgtgaa aattacccta
     1081 accggcgtgg actttttcgc ggtgacggaa tgatacacgt cctcataaaa aacggtctgg
     1141 ccttcgagca cctgccgaat gctgtcaaca atcttttgat cccgcagcgc aaacaggtcc
     1201 aggccgatca ggacttcttt ggaagacccg ataatgtcga cgaaatgatc attgcacgcg
     1261 acaatgacgc ccttttcatc aaagtaaaaa ttgcccaggg gggaatactc gaaaagcagc
     1321 cggtatttct cctcgctttc ccgcaacgcc tccttcacgc gtttttgatc cagcgcggag
     1381 cgctccagtt tcgcaatctt ttctctcagg gaagccagtt caccgagcag ggcctgcctc
     1441 gtcccggcgt gatcattcat gcggacattc tcccggccgg ccaaaagcgc ggatggataa
     1501 aaaaggtttt tgaactgcaa ccatgtcggc ctactccatg atggatattt catttatgcc
     1561 gggtggattt gtagcagaaa atggatattt gaacaagact ttgaacagat ccgatccgat
     1621 tggtcaggcg ggcgcaaaac ggtagtgggg gccgaagcgt tcaaacgcgg cacgatcgag
     1681 agcttcccgg tggtccggat gggcgatgcc gatgatcaga cgcgcccgct cctgcaggga
     1741 acggccgtac agattcaccg ctccgaattc cgtcaccagc cagtgcgcgt gggcgcgcgt
     1801 cgtcaccaca cccgcgccgg gaagcagaat gggggcgatt ttactgatgc ctttttccgt
     1861 caccgacggc atcgccagaa tgggtttgcc gcccgccgag cgcgacgcgc cgtaaaaaaa
     1921 gtcgatctgc cccccgacgc cggagtagca tttcgtcccc agcgagtcgg cgctgatctg
     1981 gccggtcaga tcaacctgca gcgcggagtt gatcgccgtg gccttcgggt ttttcgcgat
     2041 gatgaacgga tcgttggtgt agccgacgtc cttcatcagc acgcggtcgt tgcaatgcgt
     2101 gtagtcgtaa agtcttttgg accccaaaag aaacgtcgcc accatttggc cccggtcgat
     2161 ggccttgttc tctccgttga tgacgccctt ttcaaccaga tcgatcacgc cgtcggcgaa
     2221 catctccgtg tggattccaa gatttttatg cccggaaagc tgcgtgagca ccgcgttggg
     2281 aatcgcgccg atgcccatct gcagcgtcgc tccgtcttcg atgagcgaag cgcagtgttt
     2341 gccgatggcc gcctcgacag cgtccggcaa agcgaagtga accgtttcca gcggcgcgtc
     2401 gtcttccacg aaaaaatgga tgcgggaggc gtgaatgagg ccgtcgccgt acgtgtgggg
     2461 cacatgcgga ttgatcacgg cgatcacgac cctggccttt tcgacggcgg ccacggacgc
     2521 gtccaccgat gtccccaggg agacgcgccc ccgttcgtcg ggcggactca cctgaatcag
     2581 ggcgacgtcg cacggaaggt atccttcgcg atagagacgc tgggtttccc ccagaaacac
     2641 ggggatgtaa tcggcgtatc cctgctgcgt cgagcggcgg gcattggggc cgacaaaaaa
     2701 ggaatcgagc tgaaagacgc cttcaaacgc cggatcggcg taaggcgcgg ggccttccgt
     2761 gtggagatga tgaatgcgga cgccgcgaag ttcgcccgag cggccgcgcc ggcacagcgc
     2821 ctcgaccagg cacgcggggg tgttggacgt cgcgctgata tgaacgtgat cgccggagcg
     2881 gacagccctg acggcttcgt caggcgtgac gggttgaatg gaatttgctc tcatggacac
     2941 ccatttttta tgggttgcta tggccccaac gcccttccga tcgtcaagga cacggaaaaa
     3001 gactgaagac tgaaggccga aggtatattt tgcaggcctt cggtctaatt tcctttcaaa
     3061 aattctctta tatcgtttga aagccggtcc ggcttttcca gatggatcgc gtggccgcag
     3121 ttctcgtaaa ttaccatctt tgcttgcggc atcttctggc ggaaatcttc cgccagggca
     3181 attggaaaaa tcgggtcctc ccggcccaga acgatcagcg tgggctgatc gattttatgc
     3241 acccgctcgt tgctgtcaaa cagaccggcg gccgtaaact gcctcacaaa ggcatggaac
     3301 ggctgcggat ttttcatgcg cagctcaacg gccttgtcca gggtatccgg ttgctgttcc
     3361 acaaaacccg gcgcggtcat cagcgtcaga tcgatgcgat aaatttccca gggcttcttt
     3421 ccggcaatgg cggcggccgt cctgtcccag aaaggctttc ccagcgtcag cgaccggtgg
     3481 ccgcccatgc cggtcgccac cagaatcagc cggttgacca tttccggata ggaaagcgcg
     3541 atttcctggg aaatgaagcc gcccatcgag aggccggcca cgtgggcctt ttgaatgccc
     3601 agcgcccgaa gcaggcccac cgaatcagaa gccatcaatt ccatggaata cacatcgtcc
     3661 ggcttgtcgc ttcggccgat gccccggttg tccatggcaa taacccggaa gtctttggaa
     3721 agctcctcga tctgcgcaaa ccacaagtcc atcgggaaag ccaggccgtc gaggagcaag
     3781 agcggctctc ccgttccctt ctcttcgtaa tagattttga tgtcgttgac ggtttgatgc
     3841 ggcatgaaca actccataaa aatataggga acggtctcgt gacctttagg tgattgaaac
     3901 cgttccctac gatataaaat attgattaaa ccgcttcgat ggcaatcgcc atcccctgtc
     3961 cgccgccgat gcacagggaa gccagcccca ggcccacgtt gcggcgcttc atctcattgg
     4021 ccagcgccag cacgatgcgc gctcccgagc agccgaccgg atgaccgatg gaaatggccg
     4081 agccgttgac gttggtgatg tcccaaggaa tattgagctc gcgcagacag gaaatggcct
     4141 gggaggcaaa cgcttcgttg agttcaaaca ggccgtaatc cttgaccgac gtgccggtga
     4201 tgcgcagcag tttgcggacg gcgggaacgg gccccagccc catgtaagcc ggatcgacgc
     4261 cgcccgaggc gtagcccttg atgcgggcca ggggtttcac ccccagcgct ttggctttgt
     4321 cctcgctcat gatcagcatc gcaaccgccg cgtcgttgat gccggaggcg ttgccggccg
     4381 ttaccgtgcc gtctttcttg aaggccgtgc ccagcttggc cagcttctcc atgctggttt
     4441 ccatgggacg ctcatccgtg tcgaacacga tggggttgcc cttcttctgg ggaatcgtca
     4501 cgggaacaat ttcatctttc aaaaggccgc ttttgatcgc attgcgcgca cgggtatggc
     4561 tctccaggct gagtttgtcc tgctcctcgc gcgaaatgtt gtagcgggcg gcgatgtttt
     4621 ccgcggtaac gcccatgtga tagccgtaga agatttcata gagggcgtca aacaccatca
     4681 gatcgacggt tttgccgaac ggcatgtcca tacggtggcc ccagcgggcc ttgggcaggc
     4741 cgatgggcac gtcgctcata ttttccatgc cgcctgcaat caccacttcg ttgtcgccgg
     4801 ccttgatggt ggcggcggcg agcgcaatgg ctttcatgcc ggaggcgcat accttgttga
     4861 cggtgatggc ggtcgtttcc ttgggaaggc cggcgcggat catcgcctgg cgtccgacgt
     4921 tctgcccctg gcccgcgccg atgacgttgc ccatgatcac ttcgtccacg gcaatttccc
     4981 ggagattggc gtcccacctg gaatactgct gctcgaccgg gcaaaggccc tcgtttttgg
     5041 ccgtatccgg cgcgtcttct ttggcatggg ccggcaaaac cggcttgaaa ccggctttga
     5101 ttaacgattc acgaatcacc agcgctccca gatctttggc gggaacgtct ttcagggaac
     5161 cgccgaaggc cccaaccgct gtgcgcacac cgctgacaat gactgcattt gacatgattc
     5221 tgccacctcc ctcgatagga ttgatatcgt tgatgttatt tttacaaccc ggcaaaacca
     5281 aaagcgcgta atgctttacg aatcggccgg attcgcggcg aagtattcca caatccgggt
     5341 gttatggcaa gaatgaattg cgaggcggcg cgcttacttt cttgcggccg catccgtatc
     5401 cgcaacctcc tgcgtcagat caaaacgcaa aaccagcgca tcgttttcat acgatttctg
     5461 tatgggcaat tttgattttt caaagacttt catcatggat tcattgcgcg gcagcacata
     5521 ggcgataaac cattgaattc ctcggtcccg cgcgattttg atcagatact taaacaaaaa
     5581 ggtggccacg ccccttccct gaaagtcctc atcgacaatg aacgcaatct catacgcgcc
     5641 ggcgcttttg tcataggcat aacgggcttc cgcgatgatc ctctcattcc gccctttttc
     5701 cgcgacggcc acgacggaca aaaccttgtc ataatcgatg ctcacatacg tctgcatttc
     5761 cttgtggggc atgaccggct tcggagcaaa ataacggaaa tatttcgatt cgtcggaaaa
     5821 gttgtagaag agccgccgca tcttgtcctc atcggagggc ttgatcggac gaaccttgat
     5881 ttccagagag tccttcaggg atttgcgcgt ttccaacagc gccggatagt tggccgcgtg
     5941 gcgggcgaca taaatctggt ccgcataaac ataattgagc gctttggcct gcctgagcag
     6001 gtcttcgcga tggtcggggt gggcgatgtc gatcatggcc agcgcccttt cccgaatgga
     6061 cttcccgaaa agattggcca cgccgtattc ggtgacgatg tatttcacga cgccgagtgt
     6121 gctgtgggtg ttgtcggtcc ggccgtcaaa cttgatgacg atgttgctgt ttccctccgt
     6181 atccacggaa ttgagcgcca cgatcgcctt gcctcttcgc gtcatggtcg cgccgacggc
     6241 gaagttcagc ttgctttcgt agccggtcag cagattgtcg ccggaatgga agatgaccga
     6301 ttcgccggaa atgtctatct ttttcacgtt catgatgccg atgaggcttt tgatccggcg
     6361 caccacgaaa ggattgttga gcctcgcgat cggatagaat tcaaagatgg ggttgcggtg
     6421 aacgtaccgg tagagcgcct tgctgccgta gcaatagctg gcggtaatca gtccgccgtt
     6481 gaaccggctg cggtccagcg aaatggcgcc ggattcaatc aggtccaccg cccagtcgga
     6541 aaccacatgc gttacgatgc cgaggttgcg cttttgcttc aggtgatcgg ccacggcgtt
     6601 gaacagtctg cccacgtgca gcacgacggt cgccccgtcg tcgatcaggt tggagatatt
     6661 ccagccgatg cggtccgtaa tctcgtcata cggccggttg atccgctcga ccagcggcag
     6721 atcgctttcg acgacatagt cgatgctgct gatgtggatg gtcgtttcgc ccagcgtcac
     6781 cggcatgttt ggattgattt cggcgatcac cagcgccgcc tgcttgatga cgatattggc
     6841 cacgtccacc gcaacaccca gggacatgaa gcccttgtcg tccggcacgg acgtctgaat
     6901 gatcgcgacg tccaccgcca ccgcgccgct taaaaaaatg tacggcagct ccatcaggtt
     6961 ggcggggatg aaatcgaccc ggccctcggc gacttccttg ctgatgcttt ctccgacgtt
     7021 gaacgttttc agccggtact ggtcggagct ctggtgagac aggtatttgc gcagcgtaat
     7081 gagctggatg atctccaggt cgcgcagatt tttcgcgtcc gaccgggcca gcgccgtcag
     7141 gactttcccg ggcgccgcga caccgctgga taaaaagatc ttctgcccgg gcttcagctt
     7201 ttccagcacc tgatccgtgc cgatcacttt gctgcgataa tcgaagaaac ccgccatggt
     7261 gcagtctttc atagggatgc cctccatcag cccgcgccac gttaaaatat ttcgtcaaaa
     7321 taagcaaatc ctgattgcag actatgtgct tgacagccgt tgcgtgagaa aagtataacc
     7381 ttcccatcgt caggacggga tcacgacgac ggcaaggaag ggcgtttatg aaaaaaatcc
     7441 gaaaagtact ggtggccaac cggggggaaa tcgccctgcg catcatccgg acggtcaagg
     7501 aaatggacct cgataccgtg gctgtctatg aaaaacccga cagagaagcc tattttctgc
     7561 gatttgccga tcaggccatt ctgatgggag acgggccgcg cagggattac ctcaatatcg
     7621 ataaaatcat ctgggccgcg cagaaatccg gctcggacgc cattcatccg ggctacggtt
     7681 ttttgtcgga aaatcccgat ctggcccagg cctgcgtcga tgcgggaatt tcgtttatcg
     7741 gacctccccc ccaggtgatc cgcgatctgg gcaacaaggt cgtcgcgcgc aacatcatgg
     7801 aaaaggcagc cattcccttt gttccgggaa ccggcacgct ttccgcaggc gacgcgggcc
     7861 tcacggaggc cgtggccttc ggcaagacgc acggctatcc gatcatgctc aaggcgctgg
     7921 ccggcggcgg gggacggggc atccgcaaaa tcgacagcga ggaagagctg cgctcgcagc
     7981 ttccgcaggc gcgctccgag gcgcgttccg ccttcaacga cgaccgcatc tacattgaaa
     8041 aatgcgtgga gtcgccccgc cacgtcgaag tgcagattct ggccgatgcc tacggccatg
     8101 tgatgcatct gggcacgcgg gactgctcca ttcagcgccg ccaccagaag ctgctggaga
     8161 tcgcgccggc cgacctgccg aaaaacgtgc tcgacgccat gcaccaaacg gccattcggg
     8221 cggcccgtga agccggttac gtcaacgccg gcacggtgga atttctggta gacccgaaaa
     8281 ccaatcaatt ctggttcatg gaggtcaaca cccgtctgca ggtggagcac accgtgacgg
     8341 aaattctcac gggcatcgac atcatccgcc agcaggtgct gctcgccgaa ggcggccgcc
     8401 tggaaattcc cgaagagcgc attcatctgg tcaacaaggc catccaggtc cgcatcaacg
     8461 cggaggatcc gaaaaacaac ttcatgcccg aaggcggcaa gcgcgtcgaa gtgtatcaat
     8521 cgcccggcgg accggaaatc cggctcgacg gcatcgtcta tcagggctac aaaattccca
     8581 ccgattacga ttcgctcctg gtcaagctca ccgtgcgcgg ctattcctgg cagcagacga
     8641 ttcagcggct caagcgcgcc ctcaaggact tcctgattgt cggccccaaa accacgattc
     8701 ccttttacct gtccatctgc gacgatgcgg atttccggga cggccgcttt acgaccgact
     8761 acctcgatgc ccatcccgaa ctgttcgatt atccggagcc ggagcgggaa atcgccaggc
     8821 tgtccaaact gatcgcggaa atccactcgc ggaaaatcaa tccctacgcc tactgaacgg
     8881 gcaaagctgc aaggagacct atcgcatgaa aatcctcccc gaagaaagaa tcaccccccg
     8941 cgaacttcag cgcgccgggg tcaaagccgt cctggagaaa atgcgcggac aaaaagggta
     9001 ctatctcacc aataccgaga gggatctttc gcagtcggat ttcaaaaacc ggatcatgcc
     9061 ccacacccag atcctcgtcg ccccggagcg caacgcggcc ggctactttt cgctggaaat
     9121 cacgggcgga gcttcggtcc atgtcgatat gctgcgcaaa cagatgaatc cgctggagaa
     9181 actcgaggtc cttgccgtcc acatgccgga tacgcttttc caaaccctct gccgcggcat
     9241 caacctgttc ggctaccgcc cgtatccgga aaacgtcatc cgcctcaccg tggaaacctt
     9301 tgcccgccat gtccacgtct ggcgggtgtt tgacttttta aattacatcc ccaacatggt
     9361 ccccgtttac gaagaagtcc aaaaagcggg gcgcatgctc gaggcggccg tctgcttttc
     9421 gacggggccg gagcatacca accggttcta cgtcaaaaaa gtgggcgaaa ttctggacgt
     9481 gaccggaccg gatattctgc tggccatcaa aaaccacggc ggccttggat cgcccgggcg
     9541 catcggcgaa ctggtgcgcg ccatcctgca gaagtatccc gacctgctga ttcactacca
     9601 cggacacaac accgacggca acgaaatcgg ccgcatcgtg gccgccgtgc agaacggagc
     9661 aaaaatcgtg gacgcgagcg accacgcgct gaccggcttc tacggacccc cgccgctcct
     9721 gacggtggtc aacacgctgg aagattacgg ctatcccgcc gtcgggctgg acaaacaggc
     9781 cgtcatcgac gcgtccagca aactgcggca cgaacgcccc tgctaccgcg atttcgaatc
     9841 gcagtttttc ggatttgacc cgacggtgca gacccacaaa ctgcccggcg gcgcgacggg
     9901 gtccagcttc gaacaggcgc aaaagggcgg cttcctggac cgcatgccgg aaattctgca
     9961 gaacgagctg ccccgcgtgc aaatcgagct gggcaacttc tggagcgtca cccccggttc
    10021 gcagatcctg tggaccacgg ccgccaacaa tgtcgtgaag ggggcgcgct accgggacgt
    10081 gagcgatgat ttgaaaaatc tgctgctgga gcgctacggc aagtttccgt tttacaagcc
    10141 ggcgccggag atctacgaag cggtcttcgg aaaggactgg aaaaaaatcg taaggcgcga
    10201 agccggctac cagaaaatcg aagacgtcga tctggacgtg gaaagaagaa tcctggagca
    10261 cgcgatcggc cggaaggcca ccgacgacga actggtgctg tatctgcagc atcccaacga
    10321 cgcggtgggc ttcttcaaat tcgaggaaaa attcggcaaa acatgggtgc tgccgcccaa
    10381 agtgtggttc cggcgcggcg gtttcgccat cggcgacaag ttcgagttcc ccgacgccac
    10441 cggcaaactg cacaccattg aaatcggccc ttcccgccgc accaaagacg gctcgcacat
    10501 gacgcatctg ggcgttgatt accaccccga accgatcgtc acgccggccg aagaggaagg
    10561 agaaaagcct aaggccgcct tggcgctttc cgccaaggaa atcggggcgc tggccagaat
    10621 gggcgacatt cgcgcaaccc tgaagggcgt cgtcaaccag atcccggtgg cggtgggcga
    10681 cgaggtcggc gccggcgaca cgctggtcgt tctggaagcc atgaaaatgc tcaccaacat
    10741 cgcatcggac gtggacggca aggtctcgga aattttttcg tctcccggtt catccgtgga
    10801 gaccggcgac aaactgatgc tgattgaaat ctagtaaacg ggaatgtttt tccggaacat
    10861 gtccacgatg atgttttcca tggaataaag gcccggcgga gcgtggatca gccatttggc
    10921 ggcaaaaatg gcgccctggc cgaatgccgc ccggctgatg ctctcatgca ccagacggat
    10981 ggtctggttg ggcagtccga aaatgatttc gtgcttgccc acaattcctc ccacgcgaat
    11041 ggaattgacg cgcctttcct tttcgaggcc cagaatctcc gcgattttca aagcggtgcc
    11101 ggattcctcc tttttgccgc gaaagtgctc ttccacgatt tcaatatccg cgtgcggtgc
    11161 gattccctga aggatctgcg acgccaccag cagaaaattg atgcccaggg tgatgttcgg
    11221 cgaatacaga accgccgtct tttcggacat ccgctttaaa tcctccaggt cttccgccct
    11281 gtacttcgag atggcggaaa tgatcctgat cccaaaatcg gcggcgggag cgtactgccg
    11341 gtgcgcggcg gcgcttgaaa aatcaacgat gatgtccacc gggtggcgcc ggtaaaaatc
    11401 aggggtcgat tcatccactg aaaaaaccct cgcgtcatcc acttcatacc ccagcaggcg
    11461 ggatgcgtat ttgtgatgct ccttgtgggt tttccggacc acccactcca ggtcgaactg
    11521 gccgtcctga aaaaattctt ccgccaccag gcggccggtt ttgccgaagc cgaaaagacc
    11581 gatcctgatc cttgtttgat tcgcgacttt tttgattttc atgggcaaaa tcctcctctg
    11641 cgcacgcggg cagcccccca aggctgccgc tcataaggaa tcttccctgg gagaagccct
    11701 acatttccgg aaagactaag atgggcagca tggcctcatc gatgacgtgc acatccgtgc
    11761 tgtcaaacat cgatgcatac agcttgccgc ggtgtttatg cccgacaata atcaggtcga
    11821 ttagattttc cctggcgtat tggacgattt ctccggcggt gtcgcctttg gagacggcgt
    11881 aaacgacatc cagattttcc agcaggaatt cgggcgctgc ttccttgact ttcaaggcca
    11941 cggcatcttc atggtccgcc ggatggcggt agccggcctg cggatcattg acatagagga
    12001 tatgaatccg gcttgtgaaa cgccgcgcaa attccagcgc gtgcaccatg gaagaaaaat
    12061 gctccccgtc cagatccatc gcatacagaa tatttttaaa atcgcgcatt cttaccctcc
    12121 gaaccgccgg aattatatac catcagcaaa ggaagtcaac cttttggggc acttttgacg
    12181 gccggagccg gtgttccgaa ggacgtcatc tgcagcgcgc ccgggcgctt ccgcccgttt
    12241 atgactccag gtcccggctc aattcgatca gcgtcaaagg atttaccctg gccccgctga
    12301 tccgcacgcc ccagtgcaga tgcgggccgg tcgcgcgccc cgtggcgccg acgcttccga
    12361 ggatccgccc cttttcgact tgctctccct cttcaacccg aatctgatcc agatggaaat
    12421 acatgctgaa gatgcagctt ccatggtcca caaaaacgga tttgcccgta aagaagtgat
    12481 cggcggccag aacaaccgtg ccggcattgg ttgcccgaac cggactgcct gttttgcccc
    12541 ggatatccac gcccgtgtgc aaagagcgct cctgacggtt gatgatgctt ctctgtccga
    12601 aaggcccgac gattttgttg ttcaccggca tccaaaagga accttcccat tccggagggc
    12661 ggcattcggc cttccacaga gcgctgacgg cccgcgcttc cgtctttact cttttcaggg
    12721 aggcttcgtc cagatcgact ttggcgcggg gcagccggat tttgcgcacg ccgtaatccc
    12781 ggtcgttgac ctgtatttca aaagttcttt ccatctccag agaaggaacg cgcacggcca
    12841 ccggataaac accgggcttc tgattcagat cggcggcgat aaacccccgc cacgcaccgt
    12901 cgtcccgacc acggagaagg ggaatttcct tttccatcca taccacctga agatcgtcgg
    12961 attcacagcc ctggatgctg agcaggatca ggccgccgcg aaccattgcg tcggacgaaa
    13021 aagacacgtc cggcgaagac ccggattcgg ccggcatcgc tgccaggctc agcatcaaca
    13081 gcagcacagc ggatacccga ctcatgaaag ccgccttgcg gcccttcggt ctcgctggcg
    13141 cgcgaccctc ggcaaacggc agaatcccgc gtcttttcga ggcagccgct ccgtttgccg
    13201 gcgcgggctt cgaaatcttc cagcccgcag tcggcgtgga agcggcggaa aacccgccgg
    13261 gggaagggta aaccggcgaa tgcggtgagg cagcgtgtcg ggccatgaga ttctccttaa
    13321 aggcgttgtc cacgtcagtg tacgcaggat ttgttttttt gcaaccctga aatggcccgt
    13381 gtcttgaaaa aatgaaagcg gattgacttg cggcgccgta tcgcctatag tgtttgcgcc
    13441 ttcttgaagg aatggagagc ttcagtggat attgccgctt tttatcattc ggaaatcttc
    13501 aaatttgtcc tgcttcctgt cttgattttt cttgcgcgga tttgcgacgt cacgctcgat
    13561 acgctgcgca tcatctatgt gtcgcgggga atgaaatttc tggcgccggc gatcggcttt
    13621 gtcgaagtgc tgatctggct ggtcgcgatc acgcagattt tcaagaacct gtccaacccg
    13681 atatgctatg tggcctatgc gggcggtttc gcgatgggca actttatcgg catcatcatc
    13741 gaagaaaaaa tggcgatcgg caccgtggtc atccgaatca tcacgcaaaa ggacgcggag
    13801 gcgctcatcg gctgccttaa agaaaaccgt tacggcgtca cgcacgtgga cgctcagggg
    13861 gccatggggc aggtaaagat tatttttacc attgtcaaaa gaaagtccat cgattccgtg
    13921 ctggaaatcg ttcgcggctg caacccgctg gcgttttata ccattgaaga cgttcgttcc
    13981 gtccgcaagg gggtcttccc cctcttcaaa gccgccggca agggacccgg gccggccgcg
    14041 gacggatagg cggaccggag aagtcccgcc aactgccggg aaaactcccg tcgccgaata
    14101 gccggtcggg gagtcaccct tattccccgt ttccgttatg ccgcctgccc cgcttttgcc
    14161 tttaaatccc gccagctctt gagcgccagc acaatcacgc cgatggaaag gaggatcagc
    14221 gcgacgtccg tcacggccag cgcgaggttg tgcttgggaa ggtatttatt gaccagcagc
    14281 gacaccagcg aataaatcgt ggtgatcatc atgaaaaccg ccggcagcac ggtgaaccag
    14341 gccgttttct ttcttttgac cagccaggcg gaaacggcaa tcagcgccag cgaagccaga
    14401 agctggttgg cggagccgaa aatcggccag atggccgcaa acgcgttggt gtaggccaga
    14461 aagtacatca gcgatacgca cagaaaggcg ttgaaaagat aagaccccat gattttcgga
    14521 actttccgga aaacgacctg ccagagctcc tcaaacagat accggttcaa gcggacggcc
    14581 gtgtccagcg tcgtgacgac aaacccttcc accatcagaa ttccaaagac cgttccgtac
    14641 acgggcggaa tgccgagcga tttgtccaga aggccgccga ccccgagggc aaacgccagt
    14701 atgggattgg ccttgacgcc ggcggcggat ggaaaggcga tgttgacgaa atcggcaaaa
    14761 gcgatcccgc agccgacggc aatcatgacg ccgatggcga gcaggccttc gagcagcatg
    14821 ccgccgaaag caatcttttt gacgtgcgtt tccctggaaa cctgtttgct ggacgttcct
    14881 ccggccacca gcgcatgaaa accggaaacc gcgccgcagg ctacggtaat gaacagaaaa
    14941 ggccagaccg gccccaactt gtctgctccc tgcgccagat tgaaggcggg cgccgtaaag
    15001 gtcaggcctt ggaagccggc ggcgatgcct ccggcaaaaa gcgcggccac tccgatatac
    15061 agcaaaaagg aattggtgaa atcgcgcggc tgcagaatga tccagacggg aattcccgcc
    15121 gcgagcagcg tataaaagca gagaataacc atccagacat ccgggtccag ggaaacagga
    15181 tatttcatgc cgtacaaaat ggagcctatg cagacgatga cggccagggg tacggccagc
    15241 ttgacgctga cgcctctttt gtacaccagc cagccgacga ccggcgcaaa agcggtgatg
    15301 ataataaggg acgtcgagga gatgccgccg atttgcgcgt tgggtacgcc acccaccagc
    15361 acggttttga caatcgtctg gtcgggcgtg agcctcatca tctccagcgg caccagcgac
    15421 gtcagggaaa cggccgtcag ccccagaaag gcggaggtca gaagcaaaag catcaggatg
    15481 gcaaagctga tgaacaggaa gaagcctgtg ttgcccaggg actctctggc gatctcggcg
    15541 atggacttgc ccttttcccg aacgctggca aacagcacgg tcatgtcctg gacggcgccg
    15601 atgaagatgg tgccgataac cgcccacagc cagacgggca gatagccgaa cagcagggcg
    15661 acggtaggcc cgataatcgg accgcctccg gcgatgccgg caaaatgatg gccaaataaa
    15721 accacgggct ttgtcggcac atagtcgcga tcgtccctga gcgcgcaggc cggcgtgggc
    15781 cggttgtcat tttcgtcaaa aagctttgca ataaaacggg cataaaagcg ataggccaga
    15841 aagaacaaaa ccaccgctat gattaacaca acagcaacat tcataacctt ctcctcgata
    15901 gcgcacggat tggatggtcg ccgccattta ttgttgatga aggttgcccc gcgtgcccgc
    15961 aggacgacgg aatcatacac cttattttcc gggtttcaag aaaattttgc atggcggaaa
    16021 tgaaaattcc ggcatgcagc gtcccgcatg gcggccggtt gctgtttcct cttgacacgc
    16081 ttttgcgttc tttctatagt gccttcggcg ttttttaaaa acggtacgat gaaaatcttt
    16141 aaaatcaata cccaaggagg ataggaacat ggcttttgaa tatgaaaaac tggtggtgga
    16201 acaagacggc gcggttgtgg tggccgccat caatcatccg ccggccaacg caatggcgca
    16261 gggggttttg cgggatttaa ccgacctgct caaccgctgc gaaaaggacg actccgtgcg
    16321 cgtgctcatc attaccggca ccggcccgaa atgcttttca gcgggcgcgg acatcaccga
    16381 atttgccgac ctccagtcgg gcaaagtgcc ccagtacgac ggcaacgacg tatatttcaa
    16441 aattgaaaat tacccgaaga tcatcatcgc ggccattcag ggcctggccc tgggcggggg
    16501 cctggagctg gcctgctcct gccatttgag aatcatggcg gaagaagcgg tggtgggcct
    16561 tcccgaagtc aagctcggcc tcaatcccgg ctggggcgga actcagcgcc tgccccgcta
    16621 tatcggcaaa accaaggcgc tggagctgat gctcaccggc gacttcatgc cggccaaaga
    16681 agcgctcgcg ctcggcctgc tcaacaaggt cgtccccgcc gccgaagtcc tggaaaccgc
    16741 caaaaaactg gccaaaaaac tggcgggcgg cgctcccgtg gcacagcgcg agatcatgaa
    16801 agccgttcgc ctgggattgg agggcaccct ttacgacggg atcaccaggg ttgagaaagc
    16861 cgccttccaa accctgctgg tcaccgaaga tttcaaagaa ggcgccaagg cgtttctgga
    16921 aaagagaccg gcgcagttca agggccgtta gggaaaatgg cgtaaaaacg aaaaacggcg
    16981 gccggtttgg ccgccgtttt ttttgaggac tacagggtat agagttcccg aagatccgcc
    17041 tgcggatagg aaagctctcc gcctttgtca atgacctgat tgcggtattt tttgacggaa
    17101 agaacttcca gcgtttcaat gtgctgaatg ctcttgagct ccttggcctt gccgagcatc
    17161 tttttgtagc tgtcgtagga ttccgtgatg atttccacga taaaggagtt cgcgccctgc
    17221 acgtgcagaa tggagctgac aatgcgcatt tttttcagga gttcgatcag cttttcgtca
    17281 tggtggggat tgtcgataag catgaaaaag tgaaaccggt ccgtcagatc cttgtaatcc
    17341 ttcaggtcaa aatcgttttt ttgcttgaag acctggatga ctttctgcgt ctgcacggaa
    17401 atcacggcgg gcacgcccga ggaaagccgg agcgccttga gccggttgat ccagtcatga
    17461 agctgctccc ggtcgtcaaa attggagtcg atgaggtagc tgtgccggcc catcacgtgg
    17521 aagatggaaa cggcgtgcgg gtttcctctc agaaaagtca tcaacccgtc ttcttccccc
    17581 cagttttgca caaaaaccag cgcgtgcaat atcgtatttt tcggcatgct tcctccttcg
    17641 aatcccgtcc cggctgcgtg gaaaatgaat gccggacagc ttttgttttt acggcacgaa
    17701 gtatatgaaa caaagcgcgg aagtgtcaag cggcatttca ggagcgcggc gggccgggaa
    17761 cggaagaacc gtggcggaaa aggaaaaggg ggcggccttc acaagaatgc cgcccccttc
    17821 ctttcagcct tcagtctgca gggaacagtc gcgactgttc cctataatca attacttcct
    17881 caactccgcc ggtgttttgt agcgtttggg atccacccat ttggacacca gcttttcgtt
    17941 ttcctcgcag gactcatgca ggaaggcaat cttgccgccc tcctggaact ggacgatcaa
    18001 ctgcagaaag tagccgggat aagtcttcca gggattagcc gcgtcaactc tcacgcggga
    18061 atgatagaaa ggtttaattt tccgggtttc atatttcatc ttgcccaggg agtagacggt
    18121 ttccacatct tccatcgctt tgatcaaggt tttgatgttg ccggttccct tggctctttc
    18181 gatggctgac ttgaaatggt aaatatcggc atacgcgatg tggacatgga tctgcatggg
    18241 gattttgcgc gcatgcgctt ttttcaccag ggggatggtt ttgggcgtca gcgggatcgt
    18301 atccagatcc gtgtaggagc tgatcacgcc caagcccttg ccgcccgtca ttttccagta
    18361 atcctgggtc tgggcgacgc cgccgtagag attcacctgg atgtcccggg ccgcggaatc
    18421 agaccactgt ttggcaaagg attccgtgtc cgtaaaccac gagctgatgt agaaaatcaa
    18481 atcggcgttg gattttttga tgtcctgcag aatcggcaga tacatggtgc cgcggggttt
    18541 gaccgccttg ctgtacacca cttccagacc gcagtccttg gccatttttt cccaggtggg
    18601 cagattgatg tagctgatgc ctttgcgcca ttccgtcgtc caggccagat cctcccacag
    18661 gaaagcgatc cttttcgttt tctgatactg gggcatggtc ttgcagaaga aatatttcgt
    18721 ctgggcataa tgcgccggct ccggatccca gtcgcggaag cagtgattgt atttcggcca
    18781 ctcatcgatg atgcgcgccg tcagttcggc gcccatcggc ccgttgaaga tcagaatgtg
    18841 cgggtattcc ttgaacagca ttccggacgc ttcctgaacg gccaggcaga tttccgtccg
    18901 gccttcgacg gagatgaact tcgccttgtc ctcaatgacc agtttgcgaa acgcctcaac
    18961 ggacagagac gtgtcgccct tgttgtccat gaccacatac ttgaccggcc ggcccatgat
    19021 cccgccggtg gcgttgattt cctccgccgc cattttctgg atgtccatgc acggacgggt
    19081 gcccggcgat ccgaccatgc cgatgtagcc gatcacaaac ggctctccct tcggctgcgc
    19141 cactgcaacg gatggtaagc tgatagccat cgcaaccagc cctgccagcg ccgtcacgac
    19201 gccccacttg actctgcttg ctcttttcat catgtttccc ctcctgttta tcaaaattcc
    19261 ccttaaaaat acgatcaaat ttctgccgaa tatttgatcc ttctaatcgt ttaaatattt
    19321 tttccggata tattcattgt ccccgc
//
LOCUS       DAOPAF010000027        17447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3335_length_17447_cov_48.711189, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000027 DAOPAF010000000
VERSION     DAOPAF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3335_length_17447_cov_48.711189"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..588
                     /locus_tag="PLQ77_04330"
     CDS             238..588
                     /locus_tag="PLQ77_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HPL09936.1"
                     /translation="MAKTITTKYDVSEHLRTPQEMAAYLEMCIEESNGDAAFIAKALG
                     DIAKAKGMSQVARDAGLSRESLYKALSGDRSPGFDTILKVIGALGIKLHAAAVHSPKS
                     AAQQKQSSRSLDSG"
     gene            644..1390
                     /locus_tag="PLQ77_04335"
     CDS             644..1390
                     /locus_tag="PLQ77_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL09937.1"
                     /translation="MDIPRIFNITESAHRIHNPITPEKLATLGAALRLESGARVLDLG
                     SGSGEMLCTWARDHCIIGTGVDMSRLFTEQAKRRAEELGVAHQVQFIHGDAAGFVSDE
                     KVDVAACVGATWIGGGVAGTIELLARSLRPGGIILIGEPYWRQLPPTEDVAKGCQAHA
                     IADFLPLPELLASFGRLGCDVVEMVLADQDGWDRYEAAKWLTMRRWLEANPGDELAKE
                     VRTQLTSEPERYAAYTREYLGWGVFALMPR"
     gene            1602..2042
                     /locus_tag="PLQ77_04340"
     CDS             1602..2042
                     /locus_tag="PLQ77_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPL09938.1"
                     /translation="MHDLKLSTAVCGIDCFNCEFYYTNIDSFFEKMSEDRKNGFASRG
                     MTKEKLLCKGCRTSGCTMLAGKCETLECVKSKKLDFCFECGDFPCTKLQPLAEGADRY
                     PHNLKVYNLATIKNKGLEQWASEVTEIRAKYFKGKFKIGAGPQK"
     gene            2158..2814
                     /locus_tag="PLQ77_04345"
     CDS             2158..2814
                     /locus_tag="PLQ77_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPL09939.1"
                     /translation="MNTTVILASAVVLLCGLLLAEKKENLKGILLTKPLLSALFIATA
                     LLQPHSADPYFYLILAGLVLCFAGDICLIFFFNRKVFTAGLAFFLTGHILYVLAFFIT
                     AGFTTGAWISLAVMSVISFFVFTRLRPHLGSMVGPVIAYITIISVMVVAASSLTARQD
                     FSLQARTLVMAGSILFYVSDLFVARHRFVAKKIVNRYAGLPMYYAAQFMLAFSVGMIP
                     "
     gene            2826..>3436
                     /locus_tag="PLQ77_04350"
     CDS             2826..>3436
                     /locus_tag="PLQ77_04350"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPL09940.1"
                     /translation="MKAEIRYHNTLLNVEFPDTSRIYLSSYDTPSASAKELALEAVRN
                     PIGSESLVDSIKKRPHSNVVIAVSDITRPIPYRDFLPALLDEIASAGINRKSITILIA
                     TGMHRPSTAEERLFMFGKELAESYAIVDHDAQGEMAVLSGKSHSGAPVKLNSIFAKAD
                     YKMVIGLVEPHFMAGFSGGRKTICPGLSSLDTIKQFHGYEFLS"
     assembly_gap    3437..3536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3537..3935
                     /locus_tag="PLQ77_04355"
     CDS             <3537..3935
                     /locus_tag="PLQ77_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09941.1"
                     /translation="LPCVKNGGTMIAAGGCGEGVGSQTFETMLRKYQNDFSGFIRDIK
                     KTSDVVKDQWQIQMQARVYEKIELNKIHFLTHGIPPGHSSFLGVNIVEAASGRVAETL
                     QKTIDDLAGKGCSFAVIPEGPYCAPQSRGT"
     gene            4000..4743
                     /locus_tag="PLQ77_04360"
     CDS             4000..4743
                     /locus_tag="PLQ77_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPL09942.1"
                     /translation="MIENIKQDITAFLKPQVTELGFASADRFAEAPEKHHPGRICKNA
                     ETVIVFGITVPRGMLKSPDYNLYALHRSYHSAYMRIDEISLALCNYIESLGKCLAVPV
                     PSYAPMVFHQFEPWGILSLKHAAVNAGLGSFGRSGQTYHPRHGALLRLGAVVTDAVIE
                     ADPMLPADPCPPNCTACSKVCPSRAFDTEGRFNKMTCLGYAIKHAIYPLALSTEAGLK
                     NIERVINTAGHNYWIGCDECLKVCPLNRG"
     gene            complement(4752..5366)
                     /locus_tag="PLQ77_04365"
     CDS             complement(4752..5366)
                     /locus_tag="PLQ77_04365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPL09943.1"
                     /translation="MKLLLFDFDGVLVDSLDVYEKTVTDCLNAIGRPLARGREEFLEL
                     FEGNFYQSLEDKGVDMKQFSDASVDILAKVNYADMKPFHAIRPVLRELKNNHPLIIIS
                     SNDAPTIKEALRLYDFDGIFDDILGCDFMLSKKDKILHVIQKYNVTLQDIYYIGDTTG
                     DMKEGKQAGVKTVAVTWGWHTREKLAATAPDYLFEQPEELLKLN"
     gene            complement(5363..6406)
                     /gene="mtnA"
                     /locus_tag="PLQ77_04370"
     CDS             complement(5363..6406)
                     /gene="mtnA"
                     /locus_tag="PLQ77_04370"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPL09944.1"
                     /translation="MIRTLFWKNNTVVMIDQNALPQTECRIVCTDYRQVIAAIRNLAV
                     RGAPAIGVAAAMGAALGALALPPQPTKAFREKFHSICDEIAAARPTARNLFWALERMK
                     KRLDEVIRAGKSNPTAVLAQEARRICAEDIAINRQMGRQGSVLLSDGDQVLTHCNAGA
                     LATAGYGTALGVIRAAHAGGKKLHVYVDETRPVLQGARLTAWEMKKEKIPATLICDNM
                     AGSLMHQGKIDKVIVGADRIAANGDTANKIGTYPLAVLARVHRIPFYIAAPVSTFDFS
                     IKTGEEIPIEERRDDEVTAFRGVPAAPKGIKVYNPAFDVTPARLITAIVTEKGILAPP
                     LPAAISRLQKGRI"
     gene            complement(6403..7833)
                     /gene="gatB"
                     /locus_tag="PLQ77_04375"
     CDS             complement(6403..7833)
                     /gene="gatB"
                     /locus_tag="PLQ77_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPL09945.1"
                     /translation="MDFETIIGLEVHAQLLTDTKIFCNCSTKFGASPNSHACPVCLGM
                     PGVLPVLNKKVVEYAMKMALATNCTINPRNVFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYLMVEANGEQRKIGITRIHMEEDAGKLIHDDNNPASYVDLNRTGVPLIEIVGEPDIR
                     SPEEAAEYLRRLHEILVYLEICDGNMEEGSFRCDANVSIRPKGQKEFGTRTELKNMNS
                     FRNVQRALEYEVKRQQYLVENGEKIIQETRLWDDAQGATNPMRSKEEAHDYRYFPDPD
                     LVPVVVDEAWVAEIRAGLPELPQARRERFVNEYQIPAYDAGVLTSEKALAEYYEEVVK
                     LCGRPKIAANWVMGDVLKFLNEDKRSIRECPIAARSLADMIGLIEEGTISGKMAKEVI
                     ADMYATGKTPRGIIEKKGLVQITDEGALAKTIAAIIEANPGQLADYRGGKEKLFGFFV
                     GQVMKATQGKANPKLVNDLLRKMLAE"
     gene            complement(7833..9320)
                     /gene="gatA"
                     /locus_tag="PLQ77_04380"
     CDS             complement(7833..9320)
                     /gene="gatA"
                     /locus_tag="PLQ77_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPL09946.1"
                     /translation="MPANRIVEAFMELHQLTIHELQEKIRGGGASALQIAESVFSRID
                     AVEDRVHSYIRLMKKEAMAAAVRADEQIKKGDIQPLTGIPVALKDIVCTQGIPTTCGS
                     HILHQFVPPYNATVVEKLAEAGAVFVGKANMDEFAMGSSTETSSFGPTRNPWDLERIP
                     GGSSGGSAAAVAAGECIASIGSDTGGSIRQPAALCGVVGMKPTYGRVSRFGLIAFASS
                     LDQIGPFTRDVEDCAIMMNVLAGYDPKESTSVRAEVPDYRRFIGRDIQGWRIGIPGEY
                     FIEGIDPEVSAAVRKAIETVRQCGASIVEISLPHTRYCVAVYYIIAPAEASSNLARYD
                     GVRYGLRTADARDLAEMYEKTRMQGFGAEVKRRIMIGTYALSAGYYDAYYGKASQVRA
                     LIRRDFDEAFTKCDAILTPTSPTPAFRLGEKTDDPLQMYLSDIFTISANLAGIPGISV
                     PCGFSASGLPIGVQFLTGHFEEGRLLQVASAYEKSAAVEKRRPSL"
     gene            complement(9445..9732)
                     /gene="gatC"
                     /locus_tag="PLQ77_04385"
     CDS             complement(9445..9732)
                     /gene="gatC"
                     /locus_tag="PLQ77_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPL09947.1"
                     /translation="MKLSTSDVEYVARLARLEISEAEKDKFTAQLNDILRYIDQLNEL
                     DTAGVAPMSHAIAVTNAFREDKVVESLGTQKALANAPDARGEFFRVPKVIE"
     gene            complement(9742..10797)
                     /locus_tag="PLQ77_04390"
     CDS             complement(9742..10797)
                     /locus_tag="PLQ77_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPL09948.1"
                     /translation="MSRRTGIVKDGRYLQHATGMDHPESPERLAAVYEMLDNPHMSWK
                     YESIEPREASHEEIAYVHTLPYIQEIAGTAGKDCVFLDSDTIAGPESYEIAKLAAGGM
                     INAIDAVITGEVDNAFAFVRPPGHHAGAGNSEGFCIFNNVAIGAMHAMKKHGLKKILI
                     VDWDLHHGNGTQKMFYSDRRVLYFSTHQYPYYPGTGSLMEIGEGQALGYTVNVPLRRG
                     AVNGTFVSAFRRILEPIANAFAPELILVSAGYDIYYQDPLGGMRVTPDGFAAMARVLL
                     NIADRCCSGRVAGVLEGGYHTVGLARSAKATLEEMFDETHWTDRKLDELDEQADDENQ
                     PVIQAVIKGMRPYWNVF"
     gene            complement(10878..11903)
                     /locus_tag="PLQ77_04395"
     CDS             complement(10878..11903)
                     /locus_tag="PLQ77_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPL09949.1"
                     /translation="MKKTILPIVLFCLAAWFPLPAAHADEITLENGSAIIGTITRIDQ
                     KAILVRTDFAGVLSVDMTRVLTYTTDHPVYVAFPGHDPVRGTLTYTRKQTRIDAADGK
                     VVVSGDAPEHVWPEGATDPRARHWGFEVGLDAGGKTGNAERLSVGSRAQAKLQGPSDR
                     LLLYLRYAYAKDDGIESANQTAGGIDFEAYFREKHSWYARVEAENDQIAAVDFRATAA
                     AGYGCYFLKKTHHTLRGRTGLMFRHETLKHESSRATFGLDLGISHLYRFANAWRLTND
                     VTYTPSLQDYLDYRIYHESAFDIPVVASEVWKLRLGVSNDYNSKPAENKVYLDTTYFT
                     RLIFSWR"
     gene            12205..13149
                     /locus_tag="PLQ77_04400"
     CDS             12205..13149
                     /locus_tag="PLQ77_04400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPL09950.1"
                     /translation="MKKRIIPVGLLILLMIIACAPANSSRQRDGSNESQETRLTVSDE
                     KRLTEEALPKMRQDYPPANNPELQRYIADLGGKIVRANKLDGNPYRYNFTVVDVPDVN
                     AFAMPAGTVFVTAPLIAAASNEAELAGVVSHEIGHVVARHTAERMYAMEKAQNKTWLY
                     TAGGAVVGAAVGYGAGRLLCKDGDTSCHAQAAAIGGALGAGGGLLAQKYAFLVNSRED
                     EMEADRIGFKYSVAAGYDKNEVGKFYEKLLQMEKSRGADDSGVMKSLSDAFSTHPPSE
                     ERVQQMKELAETSPAKKGIVNTASFNRAKQIAAGLKKK"
     gene            complement(13257..14093)
                     /locus_tag="PLQ77_04405"
     CDS             complement(13257..14093)
                     /locus_tag="PLQ77_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09951.1"
                     /translation="MVKRNGITGWLFAQAMILLLLWPATVYCAAGEDQVLEAARQKVA
                     AELSRLDAGLREAAAELGKTGLAGKSARPALAKLCGKFDYAVDCAAVNLQGRMATIEP
                     EAYRRFEGKDISGQEQVRRVFKTGRPALSSVFVSVEGFPAVDAEYPVVSSGGKRIGSV
                     SVLFRPEMFLGSVFVPLTRQTSVEIWAMERGGLILHDADASQIGLNLFTSKRYQPYAG
                     LLALGKRIAARPQGKGVYEFTQGQSANVVRKNALWKTVSLYGTSWRLVAVTVEHPAGK
                     AK"
     gene            14252..14641
                     /locus_tag="PLQ77_04410"
     CDS             14252..14641
                     /locus_tag="PLQ77_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPL09952.1"
                     /translation="MSIRIVRLGTNRSPNEGLRVGTVRRPPRGVPKSEYAVQNWYDVW
                     FPNLSPSPETIKLGQQAKNPNDWKAFFRKFRAEMTTPENSKALDLLAALSHQTNFSIG
                     CYCAEEERCHRSALRALLLERGAAVER"
     gene            14747..15244
                     /locus_tag="PLQ77_04415"
     CDS             14747..15244
                     /locus_tag="PLQ77_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HPL09953.1"
                     /translation="MTRIKNRPKHTNKLLNAVRGLLEHRATWLYLLLDEAEKRGVETE
                     EFARSAIMRCGCFQGKHLADAAGTQSLRGLRKTLFTLPARMVFEMKILACDDDRLDID
                     FHYCPLVAAWREQGASDEKIAVLCDIAMQGDRGIARSFGCELVLGETIAKGHDKCEVR
                     FSRTG"
     gene            15344..15937
                     /locus_tag="PLQ77_04420"
     CDS             15344..15937
                     /locus_tag="PLQ77_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPL09954.1"
                     /translation="MEQEDLIVDVRTREEFLKEHVKGAINIPLYDVSFYTGILRGRKI
                     RAYCNTGHRAALALEKMKALGVDAAMIEPKDLDKMEKERVNILCAVNFVSARPGGEEK
                     FLQEMIGICRATEGMDGYLGSKVLEVSGVSAAGSFLSESCGDLEIIPCKYIILTYWTS
                     KEAHEKSHQIPEFLDRYAAIPAHLTQVPYEEFYEILK"
     gene            complement(16063..17268)
                     /locus_tag="PLQ77_04425"
     CDS             complement(16063..17268)
                     /locus_tag="PLQ77_04425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPL09955.1"
                     /translation="MAGAELIGQEEIKEVMDVLETGILMRYNFDQERKFVFKVRQFEE
                     EFAKYCGVKYALGVTSGSAALKIALEAMDVGPGDDVLVPAFTFLATYEAVLEVGAIPI
                     MVDIDDTLNLDPDEIEKKMTRYTKAVIPVHMCGLAANIDKIIKIARKNKLMVLEDNAQ
                     GCGASFKGKKLGSFGDMGTFSFDYVKTVTTGEGGMVITNKKNLYLRSEWYHDHGHDHN
                     PRVGRALEGRTILGFNYRMNELQGALGLAQLRKLDYLIGEQKKHKKMIMDVLAAALPN
                     VGFRAKRDPEGDSATFLAFNLPEEKLALKFQKLLSAEGLATTCYKNNKWHYVPNWEHF
                     LAFSTANSKKYPFKSKEYKGKVKYDRKSIPFAEDILGRTLVMGISVKMSPERLDTMKK
                     AIENAAKNM"
BASE COUNT         3809 a         4954 c         4691 g         3893 t
ORIGIN      
        1 atgcatcctt tatggctgca ctatcatttc cttatgccaa agtaatttca acaagcgata
       61 ttttgaagga tatggcataa ctgcagcagg tcgctacgct ccagctcatc ttttcgtcgg
      121 ccattataat ccatctaaga atctattgac aaagtgtagc cattgggcta cgttaaaatc
      181 gtgattgaaa ttcttaaaac cgcacttcgc ttggcaagaa atttgtagga gttcattatg
      241 gcaaaaacca ttacaacaaa atatgacgta tccgagcacc ttcgcacacc tcaagaaatg
      301 gctgcttatc tggaaatgtg cattgaggaa tcaaacggcg atgcggcttt tattgcaaag
      361 gcgttgggag acattgcaaa agctaaaggg atgtctcaag tggcacgaga tgccggatta
      421 tcccgtgaaa gcttatataa agccctttcc ggtgatagaa gccccggttt cgataccatt
      481 cttaaagtca tcggcgccct gggaataaag ttacatgcag ccgctgtgca ttcgcccaaa
      541 tcagcagccc aacaaaaaca atccagccga tcgctagact ccgggtgatc tcggcgtgat
      601 gcagtgactg aatctattta aacccgaaag gagattttct gcattggata ttccacgtat
      661 tttcaacatc acggaaagtg cccaccgcat ccataacccg atcacacccg aaaagctcgc
      721 cactctcggc gcggcgctgc gtctggaatc gggtgcccgc gtgctcgacc tcggcagcgg
      781 ctcgggggag atgctgtgca cctgggcacg cgatcactgc atcatcggca ccggcgttga
      841 catgagccgg ttgttcaccg agcaggcaaa acgccgtgct gaagaactcg gcgtcgccca
      901 tcaagtccag ttcatccatg gcgatgccgc aggcttcgtc tccgacgaga aggtcgatgt
      961 ggccgcttgt gtcggcgcca cctggatcgg cgggggagtc gccggcacca ttgagcttct
     1021 ggcgcggagc ctccgccccg gagggatcat cctcatcggc gagccctact ggcggcagtt
     1081 gccgccgacg gaagatgtgg ccaaggggtg tcaggcccac gccattgccg actttctccc
     1141 gcttccggaa cttctcgcat ccttcggccg cctcggctgc gacgtcgtgg aaatggtttt
     1201 ggctgaccag gacggctggg accgatacga ggcggccaag tggctcacca tgcgccgatg
     1261 gcttgaagcc aatcccggtg acgagttagc caaagaggtt cgaacccaac tgacctcgga
     1321 gcccgagcgc tacgccgctt acacgcgtga atacctgggc tggggtgtgt tcgcgctgat
     1381 gccgcggtga tgatgttctg gcaagagctg ttcattgccg tcccgggcgt gtcggcaatg
     1441 gtgatgctgg cggcgtttca tgcgctcccc gttccgtaga cggcgttttt tgtcagtcat
     1501 gaatattgaa ccaatcatta cggccaagcg ctgagttccg acggattttt tcgtcaggca
     1561 gttgcaaaac gggctgaaat cattacacca gggagaacga gatgcatgat cttaaattaa
     1621 gcacggcggt ttgcggaatt gattgcttca actgcgaatt ttattacacg aatatcgatt
     1681 ctttttttga aaagatgtcc gaagacagga aaaacggatt tgcctcccgt ggaatgacaa
     1741 aagagaaatt gctttgcaaa ggctgccgga catccggatg cacgatgctc gccggcaaat
     1801 gtgaaacact ggaatgcgtg aaatcaaaaa aattggattt ttgttttgag tgcggtgact
     1861 ttccctgcac aaaattacag ccattggcag agggcgccga tcgatatcct cataatttaa
     1921 aagtttacaa cctggcaacg attaagaaca aagggcttga gcagtgggct tctgaagtga
     1981 ccgagatcag ggcaaagtat ttcaaaggca aatttaagat tggcgccggt ccgcaaaaat
     2041 gacttgagta ataaatgatg atgtgttgat tgctgaaaaa ggaaagctgc gcgatagggc
     2101 aaccgcaaca gcgccagacg caggaaagca cctgtacaat tttatggaga gaaaataatg
     2161 aatacaacag tcatcctggc atcagccgtc gttttgctct gcggtcttct tttggcggaa
     2221 aaaaaagaaa acctcaaggg catccttctg accaagccgc tcctgtcagc gctcttcatc
     2281 gcaacggctc tgctccagcc ccatagcgcg gatccctatt tttatctcat actggcaggt
     2341 ctggtcttgt gctttgcggg cgatatctgc ctgatctttt tctttaaccg caaagtcttt
     2401 accgccggcc tggcattctt cctcacagga cacattttat acgttctggc gtttttcatc
     2461 accgccggct ttacaaccgg cgcatggatc tcccttgccg taatgtccgt catttccttc
     2521 ttcgtattta ccaggctgcg cccccatctc ggttccatgg tgggcccggt gatcgcgtat
     2581 atcaccatca tttccgtcat ggttgtggca gcttcttccc taaccgcccg tcaagacttc
     2641 agccttcagg cccggacact ggtcatggca gggtcgatcc tcttctatgt ttcagacctc
     2701 tttgtggcac gacacagatt cgtggcaaaa aaaatcgtca accgttatgc gggtctgccg
     2761 atgtactatg ccgcgcagtt catgctggcc ttttcagtgg gaatgattcc ataaggaaag
     2821 atcttatgaa agcggaaatc agatatcata ataccctgct caatgtggaa tttcccgaca
     2881 caagccggat ctatctttca tcctacgata ctccctccgc ttccgcaaaa gaacttgccc
     2941 tggaggcggt tcgaaatccg atcggctcgg aaagtcttgt ggactcgatc aaaaaaagac
     3001 ctcacagcaa tgtggtcatt gcggtatcgg acattacccg gccgatcccc taccgggatt
     3061 ttttgcccgc actgctggat gaaatcgcat ctgcgggaat caatcggaaa agcattacca
     3121 ttctgatcgc caccggcatg cacaggccca gcacagccga agagcggctc ttcatgttcg
     3181 gcaaagagct ggccgaaagc tatgccattg tggatcacga tgcacaaggc gaaatggccg
     3241 tcctttcagg caaaagccat tccggagccc cggtaaagct caacagcatt tttgcgaagg
     3301 ccgattataa aatggtcatc gggcttgtcg agccccattt tatggcagga ttttcaggcg
     3361 ggcgcaaaac catctgcccc gggctttcct ccctggatac gattaaacag tttcacgggt
     3421 atgaattcct ctcgcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgc
     3541 cgtgtgtaaa aaacggagga acaatgattg ccgcgggcgg atgcggggaa ggtgtaggat
     3601 cacagacgtt tgaaactatg ctccgcaagt accaaaacga cttctccggc tttatccgcg
     3661 acatcaaaaa gaccagcgac gttgtaaaag accagtggca gatacagatg caggccaggg
     3721 tttatgagaa aatcgaactg aataaaattc actttctgac gcatggcatt cctcccggcc
     3781 acagctcctt tctgggagta aatattgtcg aggccgcttc aggcagagtc gccgaaacgc
     3841 tgcaaaaaac gattgacgat ctggccggaa agggatgctc tttcgccgtg attccggaag
     3901 ggccctattg tgcaccacag agcaggggga cataacgctt aattttttaa ccaataaaaa
     3961 tttattgacg gtttccggtt taaaaaagga tggtggacga tgattgaaaa cattaagcag
     4021 gacattacag cttttctgaa accgcaggtc acggagcttg gctttgcatc ggcggatcgc
     4081 tttgccgaag cccccgagaa gcaccatccc gggcgcatct gcaaaaatgc cgaaaccgtg
     4141 attgttttcg ggattaccgt cccccgcgga atgctcaaat caccggatta caatctatac
     4201 gccctgcacc gaagctacca cagcgcctac atgcgcattg acgaaatatc cctcgcgctg
     4261 tgcaactata tcgaatcgct ggggaagtgc ctggccgtgc cggttcccag ctatgccccg
     4321 atggtttttc atcagtttga gccgtggggg attctgtcgc tgaagcacgc ggcggtcaac
     4381 gccggcctgg gaagcttcgg gcgcagcggc cagacctatc acccgaggca cggcgcgctg
     4441 ctgaggctgg gcgccgtggt gacggacgcg gtgatcgaag cggacccgat gctgcctgcc
     4501 gatccctgcc cgccaaactg cacggcctgc tccaaagtct gcccgtccag ggcttttgat
     4561 acggagggca ggttcaacaa aatgacctgc ctgggatacg cgatcaagca tgccatctat
     4621 cctctcgccc tctcgaccga agccggattg aaaaatatcg agagggtgat caataccgcc
     4681 gggcataatt actggatcgg ctgtgatgaa tgcctgaagg tgtgtccttt gaacagggga
     4741 tgacgaaagc gtcagttcag cttcagcagc tcttccggct gctcaaaaag atagtccggc
     4801 gcggtcgctg ccagtttttc ccgcgtgtgc catccccagg tcacggctac ggttttcacg
     4861 ccggcctgtt tcccttcttt catatcgccg gtcgtgtcgc cgatatagta aatatcctgc
     4921 agcgtgacgt tgtatttttg aatgacatga aggatcttgt cctttttgct gagcatgaaa
     4981 tcgcacccca gaatatcgtc gaaaattccg tcaaaatcat acaggcgcag agcttccttg
     5041 atcgtcggcg cgtcgttgga cgaaatgatg atcaggggat ggttgttctt cagctcccgc
     5101 agcaccggcc ggatggcgtg aaaaggcttc atgtccgcgt agttgacttt tgccagaatg
     5161 tccacggacg cgtctgaaaa ctgtttcatg tccacgccct tgtcttcgag cgactggtag
     5221 aagttgcctt caaaaagctc cagaaactct tcccggccgc gcgcgagcgg ccggccgatc
     5281 gcgttgaggc agtcggtcac cgttttttca taaacatcaa gcgaatctac taaaacgccg
     5341 tcaaaatcaa aaagcagcag cttcatattc ttcctttttg aaggcgggaa atggccgccg
     5401 gcaggggcgg cgccagaatt cctttttccg tgacgatggc cgtaatcagc cgcgccggcg
     5461 tgacgtcaaa tgcggggttg tacaccttta tgccttttgg cgcggcgggt acgccgcgaa
     5521 aggccgtcac ttcatcgtct cgtctttctt cgatgggaat ttcctctccc gttttgatcg
     5581 aaaaatcaaa cgtggaaacc ggagcggcaa tataaaaagg aatccggtga acccgcgcca
     5641 gaacggccag cgggtaagtc ccgattttat tggctgtgtc tccgttggcc gcaatccggt
     5701 ccgcgccgac aatgacctta tcgatttttc cctgatgcat cagggatccc gccatgttgt
     5761 cgcaaatcag cgtggcggga attttttcct tcttcatttc ccaggcggta agccttgccc
     5821 cctgcagaac cggacgcgtt tcatccacat agacgtggag tttctttccc ccggcgtgcg
     5881 ccgcgcgaat cacgcccagc gccgtgccgt agccggcggt cgccagcgcg ccggcgttgc
     5941 agtgcgtaag cacctggtcg ccgtcggata aaagaacgct tccttgacgg cccatctgcc
     6001 gattgatcgc aatgtcttcg gcgcaaattc gcctggcctc ttgcgccagt accgcggtcg
     6061 gattgctctt tcccgcacgg atgacttcgt ccaggcgctt tttcatccgc tccagcgccc
     6121 agaaaagatt gcgcgccgtg ggccgcgccg cggcgatttc atcgcaaatg gaatgaaact
     6181 tttcccggaa cgccttcgtt ggctgaggcg ggagcgcaag tgcgcccagc gccgcaccca
     6241 tggcggcggc gacgccgatt gcgggcgcgc cccgcaccgc cagattccga atggcggcaa
     6301 tgacctgccg gtagtccgta catacgattc gacactccgt ctgcggcaga gcgttctgat
     6361 cgatcatcac gacggtgttg tttttccaga acagggtcct gatcattcgg ccagcatctt
     6421 tctcagcaga tcgttgacaa gcttcggatt ggccttgccc tgcgtggcct tcatcacctg
     6481 gccgacgaaa aatccgaaga gcttttcctt gccgccccga taatccgcga gctggccggg
     6541 gttggcctcg atgatcgccg caatggtctt tgccagcgcc ccttcatcgg taatctgcac
     6601 caggcccttt ttttcaatga ttccccgcgg cgtcttgccg gtcgcataca tgtcggcaat
     6661 cacttccttg gccatcttgc cgctgatggt cccttcttca atgaggccga tcatgtccgc
     6721 gagcgaacgt gctgcaatcg ggcattcccg gatgctccgc ttgtcctcat tgagaaactt
     6781 cagaacatcg cccatgaccc agttggcggc gatcttgggc ctgccgcaga gcttcaccac
     6841 ctcttcgtaa tattccgcca gcgccttttc cgaagtgagc acgcccgcgt cgtaggcggg
     6901 aatttgatac tcgttcacaa accgctcgcg cctggcctgc ggcagctccg gcaagccggc
     6961 gcggatttca gccacccagg cttcatccac aaccaccggc accaggtcgg gatcggggaa
     7021 atagcggtag tcgtgcgctt cttccttcga gcgcatgggg ttggtcgccc cctgcgcgtc
     7081 gtcccacagg cgcgtctcct gaataatttt ctcgccgttt tccaccagat actgctggcg
     7141 cttgacttca tattccagag cccgctgcac gttgcggaag gagttcatgt ttttcagttc
     7201 cgtgcgcgtg ccgaattcct tctggccctt ggggcggatc gacacgttgg cgtcgcagcg
     7261 gaaagagcct tcttccatgt tgccgtcgca gatttccaga tacactagaa tttcatgcag
     7321 tcgccggagg tattccgccg cctcttcggg tgagcggatg tccggttcgc cgacgatttc
     7381 aatgagcggc acgccggtgc ggttcagatc cacgtaactg gccgggttat tgtcgtcatg
     7441 gatcagcttg cccgcgtcct cttccatgtg gatgcgggta atgccgatct ttctttgttc
     7501 tccgtttgcc tcaaccatga ggtacccgtg ctccgcgagc ggataggcgt actgcgaaat
     7561 ctggtagccc ttgggcaggt cgggataaaa ataattcttc cttgcgaaga cgtttcgggg
     7621 attgatggtg cagttcgtcg ccagcgccat cttcatggcg tattccacga ctttcttgtt
     7681 gagcaccggc agaacgcccg gcatgcccag gcaaaccggg caggcgtggc tgttgggcga
     7741 cgctccgaac ttggtggaac agttgcagaa aattttcgtg tccgtgagca gttgggcgtg
     7801 aacttccagc ccgatgatgg tttcaaaatc cattataaag aaggcctcct tttttccacg
     7861 gcggcgcttt tttcataagc cgaagccacc tgcaacagcc tcccctcctc gaaatggccg
     7921 gtcagaaact ggacgcctat cggcagaccg gacgcgctga agccgcaggg aacggagatg
     7981 cctggaattc ccgcgagatt ggccgaaata gtaaaaatat cggaaagata catttgaagc
     8041 gggtcgtccg ttttctctcc cagcctaaac gcgggtgtcg gcgacgtggg cgtcaggatc
     8101 gcgtcacatt tcgtgaaggc ctcgtcaaag tcccggcgga tcagcgcccg cacctgcgag
     8161 gcttttccgt agtaggcgtc gtagtatccg gcggaaaggg cgtaggtgcc gatcatgatc
     8221 cggcgcttca cttccgcgcc gaagccctgc atccgggttt tctcatacat ttcggcaagg
     8281 tcgcgcgcgt ccgccgtccg caaaccatac cggacgccgt cgtagcgcgc caggttggag
     8341 gaggcctccg ccggcgcgat gatgtagtaa accgccacgc agtaccgggt gtgcggaagc
     8401 gaaatctcca caatcgaagc gccgcactgc cgaaccgttt caatcgcttt ccggacggcg
     8461 gccgacactt ccgggtcgat tccttctatg aaatattctc cgggaatgcc gattctccag
     8521 ccttgaatgt cgcggccgat aaaccggcgg tagtcgggaa cttccgcccg gaccgacgtg
     8581 gattctttcg ggtcatagcc cgccagaacg ttcatcatga tcgcgcaatc ttccacatct
     8641 ctggtaaacg ggccgatctg atcgagcgaa gaggcgaagg cgatcaggcc gaatcgggaa
     8701 acgcgcccgt aggtcggctt catcccgacc acgccgcaga gcgccgccgg ctgacggatc
     8761 gagccgcccg tatcggagcc gatggaggcg atgcattcgc cggcggccac cgccgcggcc
     8821 gaaccgccgc tggacccgcc cggaattctt tccaggtccc agggatttcg cgtggggcca
     8881 aaagacgagg tttccgtgga ggaccccatg gcaaattcat ccatgttggc cttgcccaca
     8941 aacacggccc cggcctccgc cagcttttcc acaaccgtcg cgttgtaggg cggcacaaat
     9001 tgatgcagaa tgtgcgagcc gcacgtggtg ggaatgcctt gagtgcagac aatgtccttg
     9061 agagcgacgg gaatgccggt caaaggctga atgtcgcctt ttttaatttg ctcgtccgcc
     9121 cgtaccgcgg cggccatcgc ttctttcttc atcaggcgga tataggaatg gacgcggtct
     9181 tccaccgcgt cgatgcgcga aaaaacggat tcggcaatct gcagggccga agcgccgccg
     9241 cctcggattt tttcctgcaa ttcatgaatg gtcaactggt gcagctccat aaacgcctca
     9301 acaatcctgt ttgcgggcac aaatccgtgc ttttttcagc ccggattgcc cgtttgtgta
     9361 tcagaaaatg gtttcctgcc gggcaacgac tggaaaatcg gttccaatca ttcgcgcggg
     9421 cggcagaact ttcccccggg cgcatcattc gatcactttg gggactctga aaaactcgcc
     9481 gcgtgcgtcc ggggcgttgg ccagcgcctt ttgggtgcca agcgattcca caaccttgtc
     9541 ttcacgaaac gcgttggtca cggcaatcgc gtggctcatc ggcgccacgc ccgccgtgtc
     9601 cagctcgttt aactgatcga tgtagcgcag aatatcattg agctgggcgg taaacttgtc
     9661 cttttccgcc tccgatattt ccagacgcgc cagtctggcg acgtattcga cgtccgacgt
     9721 gctgagcttc aaaacatcct cctaaaacac attccagtaa ggccgcatgc ccttgatcac
     9781 cgcttgaata accggctgat tttcatcgtc tgcctgctcg tcgagctcat ccagtttccg
     9841 gtccgtccaa tgcgtctcgt caaacatctc ttccagcgtc gccttggccg accgggccag
     9901 ccccacggta tgatacccgc cttccagaac gccggccaca cgccccgaac agcaccgatc
     9961 ggcaatattg agcaatacgc gtgccatcgc cgcaaaccca tccggcgtga cgcgcattcc
    10021 gcccagcgga tcctggtaat aaatgtcgta gcccgcggag accagaatca gctccggcgc
    10081 aaaggcattc gcaatcggct ccagaatccg gcgaaacgcc gagacaaacg tcccgttgac
    10141 cgctccgcgc cgaagaggca cgttcaccgt gtagcccagc gcctggcctt ctccgatttc
    10201 catcaggctc cccgttcccg ggtagtaggg atactgatgc gtggaaaaat acagaacgcg
    10261 ccggtcggaa taaaacattt tctgcgtccc gttgccgtga tgcaggtccc agtccacaat
    10321 gaggattttt ttcaagccgt gctttttcat ggcatgcatc gcgccgatgg cgacattgtt
    10381 gaagatgcag aagccctcgg aatttccggc gcctgcgtga tggccgggcg gacgcacaaa
    10441 cgcaaaggca ttatccacct cgccggttat gacggcgtcg atggcgttga tcatgccgcc
    10501 cgccgccagt ttggcgattt cataactttc cgggccggcg atggtgtcgg aatcgagaaa
    10561 aacgcagtcc tttccggcgg tgccggcaat ctcctgaata tagggcaggg tatgaacata
    10621 agcgatttct tcgtgagagg cctcgcgggg ttcgatgctt tcatatttcc aggacatgtg
    10681 cgggttgtcc agcatctcat aaaccgccgc cagccgctcc gggctttccg gatgatccat
    10741 tccggttgca tgctgcaagt agcggccatc cttgacaatt ccggttctgc gggacacggg
    10801 cgcctccctt cctgaagctt ggacggtcgt gtatcataaa tgattttaaa gagcaaatat
    10861 cgccttgtcc ggccggttta ccgccaggaa aagatcagcc gggtgaagta ggtggtatcc
    10921 agataaactt tattttcggc cggtttgctg ttgtaatcgt tgctgacgcc gaggcggagt
    10981 ttccagactt cggaggcgac aaccggaata tcaaacgcgc tttcatggta aatccggtaa
    11041 tcgagataat cctgcaggct gggcgtgtag gtgacgtcat tggtcagacg ccaggcgttg
    11101 gcgaaacggt acaggtggga tatgcccaga tcgagcccga atgtggcccg cgaggattca
    11161 tgcttcaacg tctcgtgacg gaacatcaga ccggttcttc cccgcagcgt gtgatgggtt
    11221 tttttcagaa agtagcagcc gtatccggcg gcggccgtgg cccgaaagtc aacggcggcg
    11281 atctgatcgt tttccgcttc cacgcgcgca taccaggagt gtttttcccg gaaataggcc
    11341 tcgaaatcga ttccgccggc cgtctggttt gcgctttcga tgccgtcatc ttttgcataa
    11401 gcatagcgca ggtagaggag caggcggtcg ctgggcccct gcagtttggc ctgcgccctt
    11461 gaaccgacgc tgaggcgctc ggcgttgccc gtctttcccc ccgcgtccaa tccgacctca
    11521 aagccccagt gccgggcccg cggatcggtt gcgccttcag gccagacatg ttccggggca
    11581 tcgccgctca cgaccacttt tccatccgcc gcgtcgatgc gcgtttgttt tctggtatag
    11641 gtaagtgttc cacggacggg atcgtggccg ggaaaagcca cataaacggg atggtccgtc
    11701 gtgtaggtaa gcacccgcgt catatccacg ctcaaaactc cggcaaaatc cgtccggacc
    11761 agaatcgctt tttgatcaat ccgggtaatc gttccaatga tggcggatcc gttttccagc
    11821 gtgatctcat ccgcgtgcgc ggccggtagc ggaaaccatg ccgcaagaca aaacaaaacg
    11881 atcggaagga ttgttttttt catgatgaac ctccttgcgg attccggcag cgacggcagg
    11941 cggggccgcc gttcgcatcg gcacctaaaa aaattctgct gataaccggt ggcatgcaga
    12001 tcttctgccg gaacggtcgc ggttaaagac cgcgcattca agtttttcac ggaatttgta
    12061 taccaaaaga agggaagagg cgtcaaacac caaaccgttg gaagaacgcg cggcggccgc
    12121 atccgcttga cagccgcagg cggtcatgct tagatcctgg cggagtcaga gaagcaaagc
    12181 attatggact ccgggaggaa agtgatgaaa aagagaatca ttccggttgg tctgctcatt
    12241 ttgttgatga tcatagcctg cgcccccgcg aacagttcgc ggcaacgcga cggctccaat
    12301 gaaagtcagg aaacccgcct caccgtttcg gatgaaaaac gccttaccga agaagcgctg
    12361 ccgaaaatgc ggcaggatta tcctccggcg aataatccgg agcttcagcg ctatatagcc
    12421 gacctgggcg ggaaaatcgt ccgggcgaac aaactggacg gaaatccata ccgttacaat
    12481 tttacggttg tggacgtgcc ggacgtcaac gctttcgcca tgcccgccgg aactgtcttt
    12541 gtcaccgcac cgctgatcgc cgccgcctcc aatgaagcgg agctggccgg cgtcgtctcc
    12601 catgaaatcg gccacgtcgt cgcgcgccac acggccgaac ggatgtacgc catggaaaaa
    12661 gcccagaaca aaacctggct ttacacggcg ggcggtgccg tcgtcggcgc ggcggtcggc
    12721 tacggagcgg gaaggctgct ctgcaaggat ggcgacacgt cctgccacgc ccaggcggcg
    12781 gccatcggag gcgcactcgg cgcgggaggt gggctgctgg cgcagaagta cgccttcctc
    12841 gtcaattcac gcgaggacga aatggaggcg gaccgcatcg ggtttaaata ttccgtcgcc
    12901 gccggatacg acaagaatga agtggggaaa ttttacgaaa aacttctgca aatggaaaaa
    12961 agccgcggcg cggacgacag cggcgtgatg aaaagcctct ccgacgcgtt ttccacccat
    13021 cctcccagcg aggagcgggt gcagcagatg aaggaactgg ccgagacaag ccccgctaaa
    13081 aagggcatcg tcaataccgc gtcgtttaac cgggcaaaac agattgccgc ggggctcaag
    13141 aaaaaatagc atatgacgcg acaacggggg cgcccgcaac agcccggctg ccggcctttg
    13201 cctgccctga agatcagggc gtgctgcaaa gttcctgttt gaagaagaca ggattcctac
    13261 ttcgcttttc cagccggatg ctccaccgtg acggccacca ggcgccacga cgtaccatat
    13321 agagatacgg ttttccacaa ggcgttcttc cggacaacgt ttgccgattg accttgcgtg
    13381 aattcgtaaa ctccttttcc ctgcggccgc gcggcaattc gttttcccag cgccagaaga
    13441 ccggcgtaag gctggtagcg tttggaggta aacaggttca atccgatctg cgacgcatcc
    13501 gcgtcatgca gaatcaggcc gcctctttcc atggcccata tctcgacgga ggtttgccgc
    13561 gtcagaggaa caaaaacgct gccgagaaac atctccggcc gaaaaagaac gctcacggaa
    13621 ccgatccttt tccccccgga gctcacgacc gggtattcgg cgtccaccgc cggaaatcct
    13681 tctacagaaa caaagacgct gctcaatgcg ggccggccag ttttaaaaac ccgccggacc
    13741 tgttcctggc cggaaatgtc tttcccctcg aaccgccgat aggcttcggg ttcgatcgtc
    13801 gccatgcggc cctgaagatt tacggccgcg cagtccaccg catagtcgaa ctttccgcaa
    13861 agcttggcaa gagcaggcct tgcgctcttt ccggcaaggc cggttttccc cagttccgcg
    13921 gccgcctcgc gaagacctgc gtccagacgg ctcaattccg cggcaacctt ctgccgggcc
    13981 gcctccagca cctgatcctc tccggccgca caataaacag ttgcgggcca aagcaacagc
    14041 agaatcatcg cctgcgcaaa cagccatccc gttattccgt ttcttttcac catttccctt
    14101 ctcctcccgt cgttgatgga aactgtcttg ctgaatgacc gccgccgtgg ttcatccacg
    14161 gagccgttgt tgacaattat ccgtgaaaac cctataagaa tcaaccggga ttgtgcaagc
    14221 gccggccgtt tttcaaccgc agggaggcaa tatgagcatt cgcatcgttc gcctgggaac
    14281 aaacagaagt ccaaacgaag gtcttcgcgt cggaacggtc cgaaggcctc cgcgcggcgt
    14341 tcccaaaagc gagtacgccg tacaaaactg gtatgatgtc tggtttccca acctatcccc
    14401 cagcccggaa accataaagc tgggacaaca ggcaaaaaac cccaatgact ggaaggcgtt
    14461 tttccgaaaa ttcagagcgg aaatgacaac gccggaaaac agcaaagccc tggacctgct
    14521 ggccgccctg tcgcatcaaa cgaatttttc catcggctgc tactgcgccg aagaagaacg
    14581 ctgccaccgg tccgcgctgc gcgcacttct tctcgagcgg ggcgccgcag tggaacgttg
    14641 acgtcgattt aagtccccgt caccatggtg tggcttcgcg tgaacacgaa aagccattct
    14701 tcaggaaacg cctggtgcgt tgcaaaatag gacaaaggag gaaagcatga cccgcatcaa
    14761 aaaccgcccg aagcacacaa acaaactgct caacgcggtc agaggcctgc tggaacacag
    14821 agcaacctgg ctttatcttc tgctggatga agcggagaag cggggcgtcg aaacggaaga
    14881 attcgcgagg tcggccatta tgagatgcgg gtgctttcag gggaagcacc tggctgatgc
    14941 cgcgggcacc caaagcctca gggggctcag gaaaaccctt ttcaccctgc cggccagaat
    15001 ggtgtttgaa atgaaaattc tggcctgcga cgacgaccgg ctggacatcg attttcatta
    15061 ttgcccgctg gttgccgcct ggcgggagca gggagcctcc gacgaaaaaa tcgccgtgct
    15121 ttgcgacatc gccatgcagg gcgaccgggg cattgcccgt tccttcggct gcgaactggt
    15181 cctgggagag accatcgcca aaggccatga caagtgcgag gtccggttca gcagaaccgg
    15241 ttgattcacc gggagtgccc cggcaacatt ttaggacggg agaagcaaaa aatcctttct
    15301 tctcccggac agaccacttt taaaataagg gagataaacc ggaatggaac aagaagatct
    15361 gattgtggat gtgcggacgc gggaagaatt tctcaaagag catgtgaaag gcgccatcaa
    15421 cattcccctt tacgacgtct ccttctacac cggcattctg cgcggcagaa aaattcgggc
    15481 gtactgcaac accggccacc gggcggccct ggccctggaa aaaatgaagg cgctgggcgt
    15541 ggacgcggcg atgatcgaac cgaaagatct cgacaagatg gaaaaggagc gcgtcaacat
    15601 cctttgcgcc gtgaactttg tttcggccag acccggcggg gaggaaaaat ttctgcagga
    15661 aatgatcggg atctgccggg ccacggaagg catggacgga tatctggggt caaaggtgct
    15721 ggaggtgtcc ggtgtgtcgg cggcgggaag cttcctctcg gaaagctgcg gcgatctcga
    15781 gattattccc tgcaaataca tcatccttac ctactggaca tccaaggaag cgcacgagaa
    15841 gtctcatcaa atcccggaat ttctggatcg ttatgccgcc attcccgcgc atctgacgca
    15901 agtgccctac gaggagtttt acgagattct gaagtagagg ccattttccg acgggcaaga
    15961 tctgtgaaag acaaaacggg attcatgcgc aattcccacg gggcgtattc tgtcgattac
    16021 gggcgcgggc gggcctttcc tggggcccgc ccgcgctgaa tcttacatat tcttcgcggc
    16081 gttttcgatc gccttcttca tcgtatccag tctttcgggg ctcattttca ccgaaatccc
    16141 catcaccagc gtgcggccta aaatgtcttc cgcaaaaggg atgctcttgc ggtcatactt
    16201 gaccttgccc ttgtattcct tgcttttgaa cggatatttt ttggagttag ccgtggaaaa
    16261 tgccaggaag tgttcccagt tgggcacata atgccatttg ttattcttgt agcatgtcgt
    16321 cgcaaggcct tcggccgaaa gcagtttctg aaatttgagc gccagttttt cttcaggcag
    16381 attaaacgcc aggaaggttg cggaatcgcc ttccggatcg cgtttggcgc ggaacccgac
    16441 attcggcaag gcggcagcca gcacgtccat aatcatcttt ttatgtttct tctgctcgcc
    16501 gatgaggtaa tccagttttc tcaactgtgc cagacccaat gcgccctgca gttcattcat
    16561 ccggtagttg aaccccagaa tggttctgcc ttccagcgcc cggccgaccc tgggattgtg
    16621 atcgtggccg tgatcatgat accattccga ccgcagatac aaatttttct tgttggtgat
    16681 caccattccg ccttcaccgg ttgttacggt tttcacataa tcaaagctga aggttcccat
    16741 atcgccaaag ctgcccagtt ttttgccttt gaagcttgcg ccgcaaccct gagcgttgtc
    16801 ttccagcacc atcaatttgt tcttgcgggc aatcttgatg atcttgtcga tgttggccgc
    16861 caaaccgcac atatggacgg gaatgaccgc tttggtgtac ctggtcatct tcttttcaat
    16921 ttcatcggga tcaaggttca gcgtgtcgtc aatatccacc atgatgggaa tcgcgccgac
    16981 ttccagcacc gcttcgtaag tagccagaaa cgtaaaagcc ggaaccagaa catcgtcgcc
    17041 gggaccaacg tccatcgcct ccagggcgat tttgagcgcc gcggaaccgg aggtcacgcc
    17101 cagcgcgtat ttgacgccgc agtatttggc aaactcttct tcaaactggc ggactttgaa
    17161 aacgaatttt ctttcctgat caaaattata acgcatcaga atgccggttt ccagcacgtc
    17221 catgacttcc ttgatttctt cctgtccgat cagttcggcc cctgccataa attcctcctc
    17281 gatgtctttg cagggaacgg tcgcgaccgt tccctgcgtg ttcgtaccgt atctttgctt
    17341 atttgaattt tagaatgctt ccgcgtaaat cgcgatcatg tcttctttgg tgatcttgcg
    17401 gggattgttc tccagcggcc gggccaccgt catcgccaca tccgcca
//
LOCUS       DAOPAF010000028        17193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3388_length_17193_cov_46.641849, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000028 DAOPAF010000000
VERSION     DAOPAF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17193
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_17193_cov_46.641849"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLQ77_04430"
     CDS             <1..298
                     /locus_tag="PLQ77_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09956.1"
                     /translation="VPDMKLLGQMAEKLNLSVEPESLFDPGNSAGRNFKALLTTEVQN
                     HLRKTIGSYEIPQKFAFIREDFTLESGMLTQTMKLKRQAVLDKYGDMLNSLYKE"
     gene            complement(381..1142)
                     /locus_tag="PLQ77_04435"
     CDS             complement(381..1142)
                     /locus_tag="PLQ77_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09957.1"
                     /translation="MAVSGAIGREDNVIHLTYEDMMMIKKEIISRNPLLNLGFENEYI
                     LQSGGLGAVLAHAGVGKTALLVQMALHAMMNEQPVLHVSLNDAVSKVDIWYRELFHDM
                     ASKNDSAEITEYWENILNYRFIMTFRVEGFSVPKLEERLTDLMQQNIFKPYAVILDGL
                     KFDESGRGLLLELKELAKKYAMRIWFTVHTHRHEPPREDGLPFSFLHVADLFDVLVQL
                     SSEGPEVYIKALKGQSPGAKKDALLLDPATMLIKA"
     gene            complement(1178..3265)
                     /gene="fusA"
                     /locus_tag="PLQ77_04440"
     CDS             complement(1178..3265)
                     /gene="fusA"
                     /locus_tag="PLQ77_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL09958.1"
                     /translation="MKFDDQKIRNIGISAHIDSGKTTLSERILYYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKNHEINIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVGGVQSQSITVDQQMKRYKVPCIAFINKCDRSGANPFRVIEQLRTKLGHNA
                     VAMQIPIGLETSLEGVVDLAAMKALYFDGDSGEDIRVEDIPPELLAEAAAKREELIDR
                     ASLFSDELTEAILEEKEITPELLYAAVRKGTLKREITPVYMGSAYKNKGVQPLLDGVT
                     YLLPAPSEVENVALDMNHNEEPVVLTSDPEKPIIALAFKLEDGQYGQLTYIRVYEGTV
                     EKGSTIVNVRNGKKVKVGRVVRMHADQMEDVDSLKAGYIGALFGIECSSGDTFVSPGL
                     NMTMTSMYVPKPVISLAIVPKDNKAQMAMSKALNRFSKEDPTFKSHVDHETNETIIEG
                     MGELHLDIYVERMKREYNAEVTTGNPRVAYRETITQRADFNYTHKKQTGGSGQFGRIA
                     GYIEPISDAEFVFENKIFGGAIPTQYIPACEKGFKQSMAKGPKLEFPITGVKVVINDG
                     AFHAVDSSDMAFQAAARGAFKEAYLRAKPVVHEPIMKVVVETPTEFQGSVMGLLNQRR
                     GMIIGSQDEGVMCVIEAQVPLGEMFGFSTILRSSTQGKAQFTMEFALYRQVPQSIAEK
                     IALEVAQNKKTAA"
     gene            4157..5434
                     /locus_tag="PLQ77_04445"
     CDS             4157..5434
                     /locus_tag="PLQ77_04445"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL09959.1"
                     /translation="MQEETQSKGARDESEAPLRGHFLSQAISGDAGQPFKVTINDITF
                     PAYLLNNNYEIDWINKDAESSIFQEEIHTIKEPTSRNIFQLLLRSRHENQQARSALID
                     FHLSFVKGKAVKPELQHLYDGMSPRERENLEKRYNCIESPPRQAVNKIPLKLSGQKGT
                     PIANDVYYMVFREGTLFIHAPKACQNQGIADLFASRSSVINDLLKQRMPTLVSFCVLV
                     ADLQNSVRICAELPPEEYFELINQVWACVEGTFKRYYGTYGKHTGDGLLYYFLKERDN
                     QYMMNAIACALEIRENMKKLSMDWKMRKGWFNELCLNIGINEGQEYFGTIPSSPNIEF
                     TALGDTVNFAGRLSDFARFGAIWATKNLINKLSVEDRSGIRFGIRHRENDREILIENS
                     FGRVMDMIVPDSQNFNKFMDIATLAITEVVEKR"
     gene            complement(5778..7541)
                     /locus_tag="PLQ77_04450"
     CDS             complement(5778..7541)
                     /locus_tag="PLQ77_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL09960.1"
                     /translation="MNTESSNPTSKATQKKLFGEGEFSSLAEAILSSAGVGIYIVQNG
                     TFVYVSPLYQKLTGYSEKELIGTRSLGYIHPEDRAAVREKAIRSLKTKSLKPYEYRFV
                     KKHQEIMWALETITPISYEGQRATLGSFMDISHRKKVETALQKSEENYRLLFESAGQG
                     ILIAKDETIQFANAALADIVGYPKKVITTRPFISFIHPDDRKIVLNRHKMRLKGEIPE
                     NDYQFRAVTSNGTVKWLQINARIITWDGVPASLSFITDITDRRQAQEELRASEEKYRT
                     IFENAQEGIYQTTCEGKFIMANQAMARILGYDAPEELLDAQSDLARRIYVNPEDRTKV
                     IEQVQREGVVRGQEIQFYRRDGRKIWISRTMRIVRDDQNNEPYLEGIVEDITEKKESV
                     DRLRKALGGTVQAIASVVETKDPYTAGHQRRVADLARAMGTEMHLSSDQIEGLRMAGI
                     IHDIGKISVPAEILSTPRRLTAIEFSLIKMHAQSGFDILKDVDFPWPIARMVLEHHER
                     MDGSGYPNGLTGNDILLESRVLAVADVVEAMATHRPYRPALGLEAALEEINSKKGILY
                     DPQVVSVCQRLFQDKNFVIKA"
     gene            7708..7926
                     /locus_tag="PLQ77_04455"
     CDS             7708..7926
                     /locus_tag="PLQ77_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09961.1"
                     /translation="MTVKKETTKKRAVKKASPAAKRAAKPDLKEFLEEVQQKAYELYQ
                     DRLRSGVAGDEISDWFEAEKAIKEKYHL"
     gene            7977..9290
                     /gene="glgC"
                     /locus_tag="PLQ77_04460"
     CDS             7977..9290
                     /gene="glgC"
                     /locus_tag="PLQ77_04460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPL09962.1"
                     /translation="MDANQEQADGQNYYGYVKTKIATEVTRQTLVLILAGGEGSRLGE
                     LTKWRAKPAVPFGGKYRMIDFPLSNCVNSGLRRIGVLTQYKAHSLIRHLQQAWSFMRA
                     EIGEFVEIIPAQQRMGKEWYRGTADALYQNIDIIHRHAPEYILVLGGDHIYTMDYSKM
                     LMQHVDSNADMTVACIEVPRLEARDFGVMSVDENMRISRFTEKPADPEPMPGKPDIAL
                     ASMGIYVFSTRFLYDSLIEDAKDETSVHDFGRSIIPRAIHSSVAMAYPYRNEKGKMAY
                     WRDVGTLDAYWKANMELCAIEPELNLYDQRWPIWTHQTQHPPAKFIFDDEDKRGQAIN
                     SLVSGGCIIAGARVKRSVIFYATRIETGSLVKDSVVLPMVDIGKNCRITRAIIDKGTR
                     IPDDTVIGEDPAEDARRFHISDDGIVLVTPEMMGQNLMFGVIAIY"
     gene            complement(9333..11027)
                     /locus_tag="PLQ77_04465"
     CDS             complement(9333..11027)
                     /locus_tag="PLQ77_04465"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPL09963.1"
                     /translation="MTYRETYLRWKECVAEPDLLAELEAIQNDDAQIQDRFLRDLPFG
                     TAGLRGVIGAGIARMNRYVVGRATQGLADYLTGVAGAGASAVIAYDSRRFSFEFARET
                     ACVLAANGIRVYLFKQLTSVPELSFAVRHLKASGGVVITASHNPGQYNGYKVYAAYGG
                     QLGPEASLAVMDRIQKLDPFTDVKRIPYAEGVEKKRILETGGEIDRIYYEQMVRLCGA
                     ENAEELKVVYTPLHGSGLRTVENVFPMAGIRNLFVVPEQRMPDGSFPTVASPNPEDPK
                     AMQMAIALAGKIEADLAIGTDPDADRMGAAVRRPDGTYTMLTGNQIGCLLIHYLLEKR
                     RLSEELRASDYIVKSFVSTGMADAIAAHYGIRCHTVLTGFRFISELIVQKEPTGAEFI
                     FGFEESFGFLAGTFALDKDGALAALLLCKAAQFFRARGRSLPDALEALYREHGYYLES
                     VKNIAFEGLDGMEKMKGIMERLRAKPIAAIGGLPVRCTEDYLSLTRTGPDGIVSALES
                     PSTDALRLLLDSDAWLCIRPSGTEPKIKIYYAVSAASRQTAENQLQDIAEDFEQLL"
     gene            complement(11077..13077)
                     /locus_tag="PLQ77_04470"
     CDS             complement(11077..13077)
                     /locus_tag="PLQ77_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPL09964.1"
                     /translation="MLINYKKEMMHDSTMPEYRDPAGAVVTGTPVVLRFRTKLENVES
                     VYLCLIGDGFHEDHQMRLAGDAWQTEITAPSIPDVYWYYFMVRVGGRLSYYGAASGRT
                     CGIGAVYSAPPPAYQLTVYNAAFEVPGWFQKSVMYQIFPDRFHFGGDSTVKKGIAHHR
                     SKGRSVYLHDTWDEPPLYAPLPGETAYNPCDYFGGTLSGIEASLDYLQSLGVTVLYLN
                     PIFEAASNHRYNTADYFRIDPVLGTEEDFRSLCRAAEERGMRILLDGVFSHTGSDSIY
                     FNREKAYDSPGAANSKDSPYYTWYTFEHYPDQYRCWWGFTSLPEINKQDSGWRRYVIT
                     GSDSVVKHWLQAGAAGYRLDVADELPDDVLAKIYAAAKQTRPEAVMIGEVWEDATTKH
                     SYGVKRRYALGGLLDSVTNYPLRSALIAFLKGQSDAFDLKHFLVDQAANYPAPLYYAL
                     TNLLSSHDVERVRTALSARIDPHQLTREQQASFVVSDSQNKKGARRQRLAAVLQFCLP
                     GVPCVYYGDEKGMTGFLDPFNRAPFTRKAPYDLTEEYRRLAGLRASADALRTGRVVYF
                     APDADCLGVLRYITGGKDALGRPAEDGVYFMAVNRSDARRLVVFDFSGKHSLLCEADQ
                     RSLRPLFNGTFTCRLTQKSFTFIDALLEISLDPLGALWLKIA"
     gene            complement(13114..15549)
                     /locus_tag="PLQ77_04475"
     CDS             complement(13114..15549)
                     /locus_tag="PLQ77_04475"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPL09965.1"
                     /translation="MKNRIEQIRGIIEGKLKRYYGRALENASDEEMFQVTAMSIRDIM
                     LERGVLANETVEKKGHKRLCYLSAEFLMGRALVNNLINLGLLAEYRAVMADIGYPFEK
                     LEEQEMEAALGNGGLGRLAACFLDSLSTLNLPVTGYGIRYEYGMFRQHIVDGQQTEVP
                     DDWTAKGDIWEVERREEQVEVRFDGTVEEVWTEGGMTCEHRDYQTVLAVPHDMPVIGF
                     DSHTPATLRLWRAECPSGFDLHSFNKGDFVCMLRQKELAESISKVLYPQDNHMTGRML
                     RLRQYYFLASATMQCIVREHKKRFGDVHTLPDKIVIQINDLHPALAIPELIRILIDEE
                     GLGWDDANVIAGRVFNYTNHTVMQEALAAWPEHVFQSMLPRVYAIVAEISERFSRQLW
                     KVFSQDRDKISRLAIIAYDEVRMANLCLAVCRKVNGVSQLHGRILKTRTFRDFYVMFP
                     GKFTAVTNGITQRRWLAAANPALCALISDHIGDKFLRDYNELERLNPLADKPRFLADF
                     AEVKRGNKRRLAEQVAKQQGIVLDPDSVFDVQAKRLHEYKRQLLKVLHILHLYNCFTS
                     DAGYALPRPVTFLFAAKASPAYWRAKSIIRLINAAAALVESHPRTRDNIRVAFLENYN
                     VSLAERLIPAADISEQISTAGYEASGTGNMKFMLNGAVTLGTMDGANVEIFERVGRDN
                     IFIFGASAGEIDRMEIDNSYRPKDLFDHNEDIRAAVSRLVDGTLPGVAPDQFQDIYQS
                     LLTGDYDRADRYFLLYDFDPYRAAFENVLAAYTDEPAWTKRAVLNTAGAGFFSADRTI
                     DEYNRQIWGLRPI"
     gene            complement(15653..17134)
                     /locus_tag="PLQ77_04480"
     CDS             complement(15653..17134)
                     /locus_tag="PLQ77_04480"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPL09966.1"
                     /translation="MNRALQEIPAEKKMKTQPSVLMIGAECAPFAKTGGLADVIGTLT
                     RELKTLEFDVRLMLPFHRIIKDRYRDRVRHLVSFSVDIGGRAQYVGVEQFDWEGIPVY
                     FIDNEHYFGHAIYCGGDFEGEQYAYVSRAAIEALPLIGFSPRILHVNDWHTAMIPMLL
                     KTQYDRLQQGRCRTVLSIHNLGYQGRFHFGFVSHLLGIESRYYHPDNIEYHGDANFLK
                     GGIVYADKLVTVSPTYAREILTAYYGEGLEGILATRAEDLTGILNGIDTNAFNPAADP
                     FIPYPYDASSLSAKQKNKEALIAELGLNLPFDAPIIGMVTRLTKQKGIDLVQRVFDEI
                     IAAGAGFALIGTGDHAYEHFFRGAAHRTGGRAVGVTQFNDPLARRIYAGCDFFLMPSQ
                     FEPCGLSQMIALRYGTLPIVRATGGLRDTVFPYNEYTNEGNGFAFENYNAHDMLHTIR
                     YALGVYENKERLASLCVRAMSQDLSFQKSALAYAKVYVDAAGK"
BASE COUNT         3828 a         4858 c         4572 g         3935 t
ORIGIN      
        1 tgttcccgat atgaagcttc tggggcagat ggccgagaag ctgaatctgt ccgtggaacc
       61 ggagagcctg ttcgatcccg gcaattccgc cggccgcaat ttcaaggcat tgctgaccac
      121 cgaggtgcag aatcatctga gaaaaacgat cggctcttat gaaattcccc agaaattcgc
      181 ttttatccgg gaggatttca cgctggaaag cggtatgctc acgcagacca tgaagctcaa
      241 gcgtcaggcg gtcctggaca aatacgggga tatgctgaac agcctgtaca aagagtaaaa
      301 cggagcttcc cgcaccggat ggaaacgcgg gagggatcac gaaaaaacgg agacgggccg
      361 tccgcctgtc tccgtttttt ttatgctttg atgagcatgg tcgcgggatc cagaagcagc
      421 gcatcctttt tggcgccggg cgactggccc ttgagggctt tgatatagac ttcgggcccc
      481 tcggaagaca gctgcaccag cacatcaaaa aggtcagcca catgcagaaa agaaaacggc
      541 agaccgtctt ccctgggcgg ctcatgacgg tgcgtatgaa cggtaaacca gatgcgcatg
      601 gcatattttt tggccaattc tttcagctcg agaagaagac cgcgtccgga ttcatcaaat
      661 ttcagcccgt ccagaatgac ggcatacggc ttgaaaatat tctgctgcat caggtcggtc
      721 agacgttctt ccagcttagg aacgctgaat ccttccacac gaaaggtcat gataaagcga
      781 tagttgagga tattctccca atactccgtt atttcagcag aatcattctt tgacgccatg
      841 tcgtgaaata actcgcgata ccagatgtcc accttgctga cggcgtcgtt taagctgaca
      901 tgcaaaacag gctgttcgtt catcatggcg tgaagagcca tctgcaccaa gagggctgtt
      961 ttgccgacgc cggcatgagc caaaaccgcg cccaaaccgc cgctttgtaa aatatattcg
     1021 ttttcgaacc ccaggtttaa aagcggattc cgtgaaataa tttctttttt gatcatcatc
     1081 atgtcctcat atgttaaatg gatgacattg tcttcccgtc cgatcgctcc tgaaactgcc
     1141 atcttttttt acttcatcct tcagtcttca gcctgtctta cgccgccgtc ttcttgtttt
     1201 gagcgacttc gagggctatt ttttccgcga tcgactgcgg cacctggcga taaagagcaa
     1261 actccatcgt gaattgcgcc ttgccctgcg tggaggaacg aaggatggtc gaaaaaccga
     1321 acatttcccc aagcggcacc tgtgcttcaa tcacgcacat gacgccttcg tcctgtgatc
     1381 cgataatcat cccgcgccgc tgattgagca gccccattac cgatccctga aactccgtgg
     1441 gtgtctccac aacaactttc atgatcggtt catgaacaac cggcttggcg cgcaggtagg
     1501 cttctttgaa agcgccgcgg gccgccgcct gaaacgccat gtccgaagaa tccaccgcgt
     1561 ggaatgcgcc gtcattgatg acgactttca cgccggtgat cggaaactcc agcttcggcc
     1621 ctttggccat gctctgttta aatccttttt cacaggcggg aatgtactgc gtgggaatgg
     1681 cgccgccgaa tattttgttt tcaaagacaa attcggcgtc gcttatcggt tcgatgtagc
     1741 cggcaatgcg gccgaactgc ccggagccgc cagtttgctt tttatgcgtg tagttgaagt
     1801 ccgcccgctg ggtaatggtt tcccggtaag ccacacgcgg attgccggtg gtcacttccg
     1861 cattgtattc acgcttcatg cgctcgacgt aaatatccag atgcagttcc cccatgccct
     1921 cgatgatggt ttcattggtt tcatgatcaa catgggattt gaatgtcgga tcttctttgg
     1981 aaaaacggtt caacgccttg gacatggcca tctgagcctt gttgtctttg ggcacgatgg
     2041 cgagcgagat gaccggtttg ggcacataca tggacgtcat ggtcatgttc agtccgggcg
     2101 aaacgaacgt gtcgccggaa gagcattcga tcccgaacaa ggcgccgatg tatccggcct
     2161 tgagcgaatc cacatcctcc atctgatcgg cgtgcatgcg cacgacgcgg ccgactttga
     2221 ctttttttcc gtttcgcaca ttgacgatcg tggatccttt ttccaccgtc ccttcgtaaa
     2281 cgcgaatata ggtcaactgc ccgtactggc cgtcttccag tttgaacgcc agcgcgataa
     2341 tcggtttttc cggatcgctc gtcaggacaa ccggctcttc gttatgattc atgtccaggg
     2401 cgacattttc cacctccgac ggcgcgggca gcagatacgt cacgccgtcc agcagcggct
     2461 gaacgccttt gttcttatac gctgatccca tataaacggg tgtgatttct ctttttaacg
     2521 ttcccttgcg gacggccgcg taaagcagtt ccggcgtgat ttccttttcc tccagaatcg
     2581 cttccgtcag ctcatcggaa aaaagggacg ccctgtcaat cagctcttct cttttggccg
     2641 cggcttccgc cagaagctcc ggagggatgt cttccacgcg aatatcctcg ccgctgtccc
     2701 cgtcgaaata caaggctttc atggccgcca gatcgaccac accttccagg ctggtttcca
     2761 atccgatggg aatctgcatg gctacggcgt tatgccccag cttggtccga agctgttcaa
     2821 tgacccggaa gggattggcg ccgctgcggt cgcatttgtt gataaacgca atgcagggga
     2881 ctttgtagcg cttcatctgc tgatcgaccg tgatggactg cgactgaacg ccgccgacgg
     2941 agcacaaaac aaggatcgcg ccgtccagaa cacgcaggga tcgctctact tctatcgtga
     3001 aatccacgtg gcctggagta tcaatgatat tgatttcatg gtttttccat tcgcaatagg
     3061 tcgccgcgga agcgatggtg atgcctcttt ccttttccag gtccatggaa tccatggtcg
     3121 cgcccacgcc gtctttgccc ttcacgtcgt ggatggcgtg aattcttttg gtataataaa
     3181 gaatgcgctc gctgagcgtg gttttaccgg aatcaatgtg cgcgctgatc ccgatgttgc
     3241 gtattttctg atcgtcgaac ttcatgatgc tttcctctca ctgactgaat ataaaaaacc
     3301 cccgcatttg gaagagtctc tgccagatgg gggatccacc gtaatcgagc cttagaaata
     3361 agggttggac gtttatatcc ttaatccgga tttgtcaagc taatgttttc attaatttcc
     3421 aggagaactt tcagcgcatc gctttgcgtg gcccggttaa acgcctcctg cacttgtttc
     3481 agcggatacc ttgcggtgac gattctgtcg aggggcttgc cgggatactt ccgcatgatg
     3541 ttcaggccgt cggcaaaccg gccgccgcca cgctttccat ataccttctt ttcaatggtt
     3601 tctgaaattt tcgatccttt tcttataaac gggagaacat atagaaaacg ggatattgta
     3661 agtcaaggag ttcttgcctg gcgttcttgc ctggtgattt ttatttgtca ataattggac
     3721 agtctaataa attgtcttag actgtataat tatacttgac aactgtgttt ttgaactgta
     3781 taagttagcc gcaacaggca caaatgtcct ttttaggaag aatgtcataa ccatgccact
     3841 ctagggggtc aaaatgaatg atgacgcacc gataaggaat caatggatta gcagcctgga
     3901 ggtcatcaat aaaacaggaa tttccagggc aacgcttaac aactacatta agatgaacct
     3961 tctgccccgt cctgccgtca aaaggccgga aacaacagat gcccgcgccc gaatgctcgg
     4021 ctattttcct gactccgtga ttgatacaat tatacagatc aaagaattaa aaaagcaggg
     4081 tttctccatg gacgccatca cggagaggtt gaaacaatct cgcttaaccc cggctattga
     4141 ttcaggagtc atgaatatgc aggaagaaac ccaaagcaaa ggggcccggg atgaatcaga
     4201 agcccctctc cggggacact ttctttctca agcgatctcc ggggacgccg gccagccatt
     4261 caaggtgacc atcaacgata tcaccttccc tgcttacctg ctgaataata attacgagat
     4321 tgactggatt aacaaggatg cggagagttc catttttcag gaagaaattc acaccatcaa
     4381 agagccgacg tcaagaaaca tctttcagct cctcttgcgc agccgacatg aaaaccaaca
     4441 ggcccgaagc gcgctcatcg actttcatct cagttttgta aagggcaaag ccgtcaaacc
     4501 cgaactgcag catttgtatg acggcatgtc gcccagggaa agagaaaatc ttgaaaaaag
     4561 atataattgc atcgagtccc cgccccggca ggccgtcaac aaaatccccc tgaagctatc
     4621 cgggcaaaaa ggaacgccga ttgccaatga tgtctattat atggtttttc gcgaaggaac
     4681 actctttatt cacgcgccga aagcctgtca gaatcaggga atcgctgatt tgtttgcctc
     4741 ccgatccagt gtgattaatg acctcctcaa acagcgaatg cccacgctgg tttccttttg
     4801 cgtgctcgtg gccgacctgc agaactcggt gcgcatttgc gccgaattgc ctccggagga
     4861 atattttgaa ctcatcaacc aggtatgggc ctgcgtggaa ggcacattca agcggtacta
     4921 cggcacctac ggcaagcata cgggcgatgg gctgctttat tattttctca aggagcggga
     4981 caatcaatac atgatgaacg caattgcctg tgcgctggaa atacgcgaaa acatgaaaaa
     5041 attaagcatg gactggaaaa tgcgcaaagg ctggttcaat gaattgtgcc tcaatatcgg
     5101 cattaatgag ggtcaggaat acttcggcac tattccttca tcccccaata ttgaatttac
     5161 cgctctggga gacaccgtca attttgccgg acggttatcc gattttgccc ggttcggcgc
     5221 gatctgggca acaaaaaatt tgatcaacaa gctttccgtg gaagatcgtt ccggcattcg
     5281 ctttggaatt cggcacaggg aaaacgacag ggagatactg attgaaaact cattcggccg
     5341 cgtcatggac atgatcgtgc ctgatagtca aaatttcaac aaattcatgg atattgccac
     5401 actggcgatc accgaagttg tggaaaagcg ctagaatcct gacatctgct cattccgaat
     5461 ccggcagatc gtaaatttta cgccgaaggc ttagcaaaaa gcataaaacg tcaaaaatat
     5521 ttcgcaaaat agctgaagaa tcatcggcat tttctgttga aaacattaac agcgcctcaa
     5581 gtatttccac agacttatca acaactgctc aacagggttg caaacaagca attccacaag
     5641 gggcaagttg gtggatcgtg atttttctcg acaagaaagt ttttttgact gaaaagttat
     5701 cttgacgatt gttttgttat ttcaaattat taagattaat caatcagttt ttactttaat
     5761 gatgggcatt ccctgattca tgctttgatg acaaaattct tatcctggaa aagcctctgg
     5821 cagacagata ccacttgcgg atcgtagaga atccccttct ttgagttgat ttcctccagc
     5881 gcggcttcca gccccagggc gggccggtag ggacggtgcg tcgccatggc ttccaccaca
     5941 tcggccacgg ccagaactct cgactccaga agaatgtcgt tcccggtcaa accattcgga
     6001 tatccggacc cgtccatccg ctcatggtgt tccagaacca ttctggcgat gggccaggga
     6061 aaatccacgt ccttcaggat gtcaaagccg gactgggcat gcatcttgat taggctgaat
     6121 tcaatggcgg tcagacgccg gggcgtactc agaatctcgg ccgggacgga aatcttgccg
     6181 atgtcgtgga tgatgccggc cattctcagt ccctcgatct gatccgaact caggtgcatt
     6241 tccgtgccca tggcccgggc caggtcggcc acccttctct ggtgtccggc ggtatacgga
     6301 tctttggttt cgacgacgga ggcaatggcc tgaacggttc cgcccagggc tttgcgcaga
     6361 cggtccacgc tttccttttt ttcagtgatg tcttctacga tgccttccag gtagggctca
     6421 ttgttttgat catcgcggac gattctcatg gtgcgggata tccagatttt ccgtccgtcc
     6481 cttcggtaaa actgaatttc ctgcccccgg acgacccctt ccctctggac ctgttcaatg
     6541 acttttgtcc gatcttcggg attgacgtaa atccgccggg caaggtcgga ctgtgcatca
     6601 agcagttctt ccggcgcgtc gtatcccagg atcctcgcca tggcctgatt ggccatgatg
     6661 aatttccctt cacatgtagt ctggtagatc ccctcctggg cgttttcaaa gatggtgcgg
     6721 tatttttctt cgcttgcgcg aagctcttcc tgagcctgtc ttcggtcggt gatgtccgtg
     6781 atgaagctca gggaggcagg cacgccgtcc caggtaatga tcctggcgtt gatttgcagc
     6841 cacttgaccg tcccattcga ggtaacggcc cgaaactgat aatcgttttc cgggatttcc
     6901 cccttcaatc tcattttgtg ccggttcaga acgatcttac gatcatcggg atggataaaa
     6961 gatataaacg ggcgggtggt gatgactttt ttagggtatc cgacaatatc ggcgagggcg
     7021 gcattggcaa actgaatggt ctcgtctttg gcaatcagga ttccctgccc cgcactttca
     7081 aaaagcagac ggtaattttc ttcgcttttc tgaagcgccg tttccacctt cttgcggtgg
     7141 gagatgtcca tgaagctccc cagcgtcgcc ctttgccctt cgtacgagat gggcgtgatg
     7201 gtctccagag cccacatgat ctcctggtgc ttcttgacga accggtactc atagggttta
     7261 aggcttttcg ttttgaggct tcggatcgct ttttccctga cggccgcccg atcctccggg
     7321 tgaatgtaac ccagagaacg ggtgccgatc agttcctttt cggaataacc ggtcaacttt
     7381 tggtacagtg ggctgacgta gacaaacgtg ccgttttgaa cgatgtaaat tccgacgccg
     7441 gcgctggata aaatggcctc ggccagagat gaaaattcgc cctcgccaaa aagctttttt
     7501 tgggtcgcct ttgatgttgg attgctggac tcagtattca agaatttccc gccttatcgt
     7561 gagtgatttc gaaaacatca tttgttcatc aatggggctt tcatacacag aataatgctt
     7621 catttcaagt aaaatacagg cattgacggc cggccggcaa aagtgctatt gaaacagcgt
     7681 ctgaaaacat caaccgagga ggcgatcatg acggtcaaaa aagaaaccac caaaaaacgc
     7741 gcggtcaaaa aagcatcgcc tgcggcaaaa agggcggcaa agcctgacct gaaagaattt
     7801 ctggaagagg tccagcaaaa agcttacgag ctttaccagg acaggctccg ctccggcgtt
     7861 gccggcgacg aaatatccga ctggtttgaa gccgaaaaag cgatcaaaga aaaatatcat
     7921 ctgtagcgtt gtactgatcc tgatggaaac cacttaataa ccggggttga aacgtcatgg
     7981 acgcaaatca agaacaagcg gacgggcaga attactacgg ctatgtgaaa accaagattg
     8041 ccactgaggt cacccggcaa acgctggtgc tgatcctggc gggcggcgag ggatcccgcc
     8101 tgggcgaact gaccaaatgg cgcgccaaac cggcggttcc tttcggcggc aaataccgca
     8161 tgatcgattt tcccctgtcg aactgcgtca attccggcct gcgccgcatc ggcgtgctga
     8221 cgcagtacaa agcgcattcg ctgatccgcc atttacagca ggcgtggagc ttcatgcgcg
     8281 ccgaaatcgg cgagttcgtg gagatcatac cggcgcagca gcgcatgggc aaggaatggt
     8341 accggggaac ggccgacgcg ctctaccaga atatcgacat cattcaccgc cacgccccgg
     8401 aatatattct ggtgctgggc ggagaccaca tctacacgat ggactactcc aagatgctga
     8461 tgcagcatgt cgattcaaat gccgatatga cggtggcctg catcgaagtg ccgcgtctgg
     8521 aagcgcgcga tttcggcgtc atgtcggtcg atgaaaatat gcgcatttcc cgcttcaccg
     8581 aaaagccggc cgatcccgaa cccatgcccg gcaagccgga tatcgccctg gcctccatgg
     8641 gcatttacgt cttctccacc cgctttttgt acgattccct catcgaagac gccaaagacg
     8701 agacctccgt ccacgacttc ggcagaagca ttattccccg cgccatccac agcagcgtgg
     8761 ccatggcgta tccttaccgc aacgaaaagg gaaagatggc ctactggcgg gacgtgggaa
     8821 cgttggacgc ttactggaaa gcgaatatgg agctttgcgc catcgaaccg gagctgaatc
     8881 tgtatgatca gcggtggccg atctggacgc accagacgca gcatccgccg gcaaagttca
     8941 tctttgacga cgaagacaag cgcggccaag ccatcaactc gctggtcagc ggcggctgca
     9001 tcatcgccgg cgcccgcgtc aagcgctcgg tcatctttta cgcgacccgc atcgaaacgg
     9061 ggagcctcgt gaaggacagc gttgttcttc ccatggtcga tatcgggaaa aactgccgca
     9121 tcacccgcgc catcatcgac aagggaaccc gcattcccga cgacaccgtc atcggtgaag
     9181 atccggccga agatgcccgg cgcttccata tcagtgatga tgggatcgtg ctggtgacgc
     9241 cggaaatgat ggggcagaac ctcatgttcg gtgtcatcgc catttactga tttgcggcgg
     9301 gtgcgtccac atgctccgcc cgaattcgaa ttttacaaaa gctgttcaaa gtcttcggca
     9361 atgtcctgaa gctgattttc cgccgtctgg cgcgatgcag ccgaaaccgc gtagtagatc
     9421 ttgatcttcg gctccgtccc ggacggtcgg atacagagcc acgcatcgga atccagcaag
     9481 agccggagcg cgtccgttga cggcgactcc agcgcagaca cgatgccgtc cggaccggtt
     9541 cgggtaaggg aaaggtagtc ctccgtgcac cggacgggca ggccgccgat cgcggcaatg
     9601 ggtttggccc tcagccgctc catgatgcct ttcattttct ccatgccgtc cagtccttca
     9661 aaagcgatgt tcttcacgct ttccaggtaa tagccgtgct cccggtaaag cgcttccagc
     9721 gcgtccggca gactccggcc tcgggcccgg aagaactggg ccgccttgca caagagcagc
     9781 gcggcgagcg ccccgtcctt gtccagtgca aaggtgccgg cgagaaatcc gaagctctct
     9841 tcaaacccga aaataaactc cgcacccgtc ggttcctttt gcacgatgag ttcggagatg
     9901 aagcgaaatc ccgtcagcac ggtgtggcag cgaattccat aatgagcggc gatcgcgtcc
     9961 gccatgccgg tcgagacaaa cgacttgacg atgtagtcgg aggcacgaag ctcttccgac
    10021 agtcggcgct tttccagaag ataatgaatc agcaggcagc cgatctggtt gcccgtcagc
    10081 atcgtgtacg tgccgtccgg ccgtcggacg gcggcgccca tccggtccgc gtccggatcc
    10141 gttccgatgg ccagatccgc ctctatcttt ccggccaggg cgatggccat ctgcatcgct
    10201 ttcggatctt cgggattagg agacgcgacc gtcgggaacg aaccgtccgg cattcgctgc
    10261 tcggggacga caaacaggtt gcgaatcccc gccatcggaa acacattttc aaccgtgcgc
    10321 agacccgatc cgtgcagcgg cgtatacacc actttcagct cttcggcgtt ttccgctccg
    10381 cacaaccgga ccatctgttc ataatagatc cgatcgattt ccccgcctgt ttcgagaatc
    10441 cgcttttttt ccaccccttc agcatagggg atccttttca catccgtgaa gggatccagc
    10501 ttttgaatgc ggtccatcac cgcgaggctc gcctccggac ccagctggcc gccgtaggcg
    10561 gcataaacct tgtagccgtt gtactgtccg ggattatggc ttgccgtgat cacgacgccg
    10621 ccgctcgctt tcaggtggcg cacggcaaag gacagctccg gaacgctcgt caactgtttg
    10681 aacagataca ccctgatgcc gttggccgcg agcacacagg ccgtttctct tgcaaattca
    10741 aaagaaaatc ttcgggaatc gtacgcaatc actgcggacg ccccggcgcc ggcgacaccg
    10801 gtcagataat ccgccagtcc ctgcgtcgcc cggccgacga catagcggtt cattcgggca
    10861 atgcccgcgc cgataacgcc gcgcagtccc gccgttccaa acggcaggtc cctcaggaaa
    10921 cggtcctgaa tctgtgcgtc atcgttctga atggcctcca gttcggccag caggtccggt
    10981 tccgcaacgc attctttcca gcgcaaatag gtttcccggt acgtcataat acctctccct
    11041 ttttgattgg ttccatctcc aggctctgtg cacccttcag gcgatcttca gccacaaggc
    11101 cccgagggga tccagcgaaa tttccaaaag cgcgtcgatg aaagtgaagg acttttgcgt
    11161 caaccggcag gtgaacgtgc cgttgaacag agggcgaagg cttcgctgat ccgcttcgca
    11221 aagaagcgaa tgctttcccg aaaaatcaaa gaccaccagc cgcctcgcgt ccgaacggtt
    11281 gaccgccatg aaatagacgc catcctcggc cggccggccc agagcgtctt ttccgccggt
    11341 aatgtagcgc aaaacgccga ggcagtcggc gtcgggggcg aagtacacca cgcggcccgt
    11401 tcgcagcgcg tctgccgaag cgcggagccc ggccagccgc cggtattctt ccgtcaggtc
    11461 gtacggcgct ttccgggtaa aaggcgcgcg gttgaaagga tcgagaaatc ctgtcattcc
    11521 tttttcgtct ccgtaataga cgcagggaac gccggggagg cagaactgca gcaccgcggc
    11581 cagccgctgg cgcctcgcgc cttttttgtt ctggctgtcg ctgacgacaa aggaagcctg
    11641 ctgctcccgg gtcagctgat gaggatcgat gcgcgcgctg agcgctgtgc gcacacgctc
    11701 cacgtcgtgg gaggaaagca gattggtcag ggcgtagtaa aggggagcgg gataattggc
    11761 cgcctgatcc accaggaaat gcttcaggtc aaaggcgtcg gactggccct tcagaaaagc
    11821 gatcagggcg ctgcgcagcg ggtaattcgt gacggagtcc agaaggcctc ccagcgcata
    11881 gcgccttttt acgccatagc tgtgcttcgt ggtcgcgtct tcccaaacct cgccgatcat
    11941 caccgcttcc ggccgggtct gttttgcggc ggcgtaaatc ttcgccagga cgtcgtcggg
    12001 cagctcatcg gccacatcga gacggtagcc tgccgcgccc gcctgaagcc agtgcttcac
    12061 gacgctgtcg gagccggtga tgacgtatcg ccgccagcct gaatcctgct tgttgatttc
    12121 gggcaacgac gtaaaccccc accagcagcg gtattgatcg ggataatgct caaacgtgta
    12181 ccacgtgtaa tagggagaat ccttgctgtt ggcggcgccc ggactgtcat acgctttttc
    12241 ccggttaaaa tagatgctgt cggagccggt atgggaaaac acgccgtcca gaagaatgcg
    12301 cattccgcgt tcttcggccg cacggcacag ggagcggaaa tcctcctcgg tgcccagcac
    12361 cggatcgatg cggaaatagt cggcggtatt gtaccggtgg ttggaggccg cctcaaagat
    12421 cggattcaaa tacaacaccg tcacgcccag gctttgcaga tagtccaacg acgcctcgat
    12481 gccgctcagc gtaccgccaa agtaatcaca ggggttatag gccgtctccc ccggcagcgg
    12541 agcatagagc ggcggttcgt cccacgtgtc gtgtaaatag acgctgcggc ccttggaacg
    12601 atgatgcgca atgccctttt ttaccgtcga gtctcctccg aagtgaaaac gatctggaaa
    12661 aatctgatac attacgcttt tctgaaacca accgggaacc tcaaaagcgg cattgtaaac
    12721 ggtcagctgg tatgcgggcg gcggcgcgct gtaaacggcg ccgatgccgc aggttcgtcc
    12781 cgatgcagcg ccgtaatagc tgagtcttcc ccccacgcgc accatgaaat aataccagta
    12841 cacatcggga atcgaaggcg ctgtgatttc cgtctgccag gcatcgcccg caagccgcat
    12901 ctgatgatct tcgtgaaagc cgtctccgat caggcacaga tagacgcttt ccacattttc
    12961 cagctttgtc cgaaagcgca gaaccaccgg cgtgcccgtg acgacggccc ctgcaggatc
    13021 gcggtattcc ggcatcgtcg aatcatgcat catttctttt ttgtagttga tcagcatcgc
    13081 tgccgtcctt ttgcgcgctt cagattgcgc acatcagatg ggccgaagcc cccagatctg
    13141 acggttgtat tcgtcgatgg tccggtccgc gctgaaaaat cccgcgccgg ctgtgttgag
    13201 cacggccctt ttcgtccagg ccggctcatc cgtgtaagcg gcaagcacat tttcaaacgc
    13261 ggcgcggtaa gggtcaaaat cgtagagcag aaagtaacgg tcggcgcggt cgtaatcgcc
    13321 ggtgagcagg gactggtaaa tatcctgaaa ctggtcgggc gcgacgcccg gcagcgttcc
    13381 gtccaccaaa cgggaaacgg ccgcccgaat gtcctcgttg tggtcaaaga gatccttcgg
    13441 gcgatagctg ttgtcgattt ccattcggtc aatctcgcct gcgcttgcgc caaaaatgaa
    13501 gatgttgtcc cgccccaccc tttcaaagat ttccacattc gcgccgtcca tggttccgag
    13561 cgtcaccgcg ccgttgagca tgaatttcat attgcccgtg ccggaggctt cataccccgc
    13621 cgtggatatc tgttcactga tgtccgccgc gggaatcagg cgttcggcca gcgagacgtt
    13681 gtaattttcc aggaaagcga cacgaatgtt gtcccgcgtg cgcggatgcg actccaccag
    13741 agcagccgcg gcattgatga gccggatgat gctcttggcc cgccagtagg ccggcgaagc
    13801 cttggcggca aacagaaacg tcaccggccg gggcagcgca taccccgcgt cggaggtaaa
    13861 gcaattgtac agatgaagaa tgtgcagcac tttcaacagc tggcgcttgt attcatgcag
    13921 ccttttggcc tgcacgtcaa agaccgagtc gggatcaagg acaatgccct gctgtttggc
    13981 cacctgttcg gccagacgcc gcttgttgcc gcgcttgact tccgcgaaat cggccagaaa
    14041 ccgcggcttg tcggccagcg ggttgagccg ctccagctcg ttgtagtccc gtagaaattt
    14101 atcgccgata tggtcggaaa tcagcgcgca cagggcgggg ttcgccgcgg ccagccagcg
    14161 ccgctgggtg atgccgttgg tgacggcggt gaatttcccc ggaaacatca catagaaatc
    14221 ccgaaaggtc cgcgtcttca gaatccggcc gtgaagctgc gagacgccgt taaccttgcg
    14281 gcagaccgcc aagcagagat tggccatgcg gacttcgtca tacgcgataa tggccagacg
    14341 gctgatttta tcgcggtcct gtgaaaagac tttccagagc tgccggctga agcgttcgga
    14401 tatttcggcg acaatcgcgt aaacgcgcgg cagcatcgac tgaaaaacat gctcgggcca
    14461 ggcggccaga gcctcctgca tgacggtgtg attggtgtag ttgaacacgc ggccggcaat
    14521 gacgttggcg tcgtcccatc ccaacccctc ttcgtcgatc agaatgcgaa tcagctcggg
    14581 aatggccagc gccggatgca aatcattgat ctgaatgacg attttgtcgg gcagcgtgtg
    14641 cacgtcgcca aagcgctttt tgtgttcgcg gacgatgcac tgcatcgtgg cggaagccag
    14701 aaaataatac tgccgcaggc gcagcatgcg cccggtcatg tggttgtcct gaggatacag
    14761 caccttggaa atcgactcgg ccagctcttt ctgacgcagc atgcagacaa aatcaccctt
    14821 gttgaaggag tgaaggtcaa agccggacgg acattccgcc cgccagagcc ggagcgtcgc
    14881 aggtgtgtgg ctgtcgaagc cgatgaccgg catgtcgtgg ggcaccgcga gtacggtctg
    14941 gtaatccctg tgctcgcagg tcatcccgcc ctccgtccac acctcctcca ccgtgccgtc
    15001 aaagcgaacc tccacctgtt cctcgcggcg ttcgacttcc cagatgtcgc cctttgccgt
    15061 ccagtcgtcc ggaacttccg tctgctgccc atccacgatg tgctggcgga acattccgta
    15121 ttcatagcgg atgccgtagc ccgtcaccgg cagattcagc gtggaaagag aatccagaaa
    15181 acaggccgcc agccggccca ggcctccgtt tcccagcgcc gcttccattt cctgctcttc
    15241 gagcttttca aaagggtagc cgatgtcggc catgacagcc cggtactccg caagcagacc
    15301 gagattgatg agattgttca ccagggctct tcccatcaaa aactccgccg aaaggtagca
    15361 gagtcttttg tgcccctttt tttcaaccgt ttcgttggcc aggacccccc gctccagcat
    15421 gatgtcgcgg atgctcatcg ccgtcacctg aaacatttcc tcgtccgagg cgttctcgag
    15481 cgcgcggccg tagtaccgtt tcagctttcc ctcaatgatt ccccttattt gctcaatcct
    15541 atttttcacc tgttcgcctt tactcttggg atcggaagca gagtccgctc cctccggaat
    15601 gggaaaccgt tttattgtac gtcccggcgc ctgtcaatgg aaaatccgcg tctcattttc
    15661 cggccgcatc cacatatact ttcgcgtagg caagcgcgct tttttgaaaa ctcagatcct
    15721 gactcatcgc ccgcacgcaa agcgaggcca gccgctcctt gttttcatag acgcccagtg
    15781 cgtaacggat cgtatgaagc atgtcgtgcg cgttgtagtt ttcaaaagcg aagccgtttc
    15841 cttcgtttgt atattcgttg tagggaaaga ccgtgtcccg gaggccgccc gtcgcccgga
    15901 caatcggcag cgtcccgtag cgcagcgcaa tcatctgcga aaggccgcag ggttcaaact
    15961 gagagggcat caggaaaaaa tcgcatcccg cgtagatgcg gcgcgccagc ggatcattga
    16021 actgcgtcac gccgacggcc ctgccgcccg tccggtgcgc ggccccgcgg aaaaagtgct
    16081 cataggcgtg atcgcccgtc ccgatcagcg cgaaacctgc ccccgccgcg atgatttcgt
    16141 cgaagacccg ctggaccagg tcaattccct tctgcttcgt cagccttgtt accatgccga
    16201 tgatgggggc gtcgaaagga aggttgaggc cgagctcggc tatgagggcc tccttgtttt
    16261 tctgctttgc ggaaagagac gaggcgtcat acggataggg gatgaacggg tccgccgccg
    16321 gattgaaggc gttggtgtca atcccgttga gaatgccggt caggtcctcc gcccgagtgg
    16381 ccagaatgcc ctccagcccc tcgccgtaat aggccgtcag gatctcccgg gcgtaagtcg
    16441 gactgaccgt aaccagcttg tccgcgtaaa cgatgccgcc ctttaaaaaa ttcgcgtctc
    16501 cgtgatattc gatattgtcc ggatgataat agcggctctc gatgcccagc agatgggaaa
    16561 cgaagccgaa atggaagcgc ccctgatagc cgaggttgtg aatggagaga acggtacggc
    16621 atcgtccctg ttgcaggcgg tcgtactggg ttttcagcag catgggaatc atggccgtgt
    16681 gccagtcgtt gacgtgcaga atccggggcg aaaagccgat cagcgggagc gcctcgatcg
    16741 ccgcccgcga aacatatgcg tactgttcgc cctcgaaatc gccgccgcag taaatggcgt
    16801 gtccgaagta atgctcgttg tcgatgaagt aaacggggat gccctcccag tcgaactgct
    16861 cgactcccac atactgggct cgcccgccga tgtccacgct gaagctgacc aggtggcgca
    16921 cgcggtcccg gtaccggtcc ttgatgatgc gatgaaacgg cagcatcagc cgaacatcga
    16981 attccagcgt tttcagctcc ctcgtcagcg tcccgatgac atccgccagc ccccccgttt
    17041 tggcaaacgg cgcgcattcc gccccgatca tcagaaccga aggctgggtc ttcatctttt
    17101 tttcagccgg aatttcctgc aatgctctgt tcattccaaa ccctttttat ttgaatccaa
    17161 ttcaaaatag acggccgcaa gcggcggcag atc
//
LOCUS       DAOPAF010000029        16942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3446_length_16942_cov_65.599988, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000029 DAOPAF010000000
VERSION     DAOPAF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16942
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_16942_cov_65.599988"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            536..946
                     /locus_tag="PLQ77_04485"
     CDS             536..946
                     /locus_tag="PLQ77_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09967.1"
                     /translation="MLVAFVFMSCSIDYLAGFWWGENRETGMARQAYVGFINEYFLPR
                     GRYNAKGLYDSLRNGLVHLFTIKDKMYELTFDEPQRHLTTSHHGYTVLDAGSFRADLL
                     AAETRYLDEVETSPPLLDKAFQRYARDGFVRWVD"
     gene            962..1684
                     /locus_tag="PLQ77_04490"
     CDS             962..1684
                     /locus_tag="PLQ77_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPL09968.1"
                     /translation="MTKLTVICNDEETEARVLHGLHNDQEFSISGAIVVRNVRAEAQN
                     FLNFLNKAIDKMPDVIILDMAVLREAAAIDMKRLMDYVRKFSSVRFIILGNRYHEQNV
                     IAMMRGGARGFVQKDHLEEDIAPSVRVVTRGEVWLSPGLLGRVCGKLLMDSQKSDMVK
                     SPTSEQLVKLNKISHREMEVLELVSESLTNEEIAHRLFLSNKTVKTHVRNIFEKTGVR
                     NRTEAALLFTRYHQDIENHLKH"
     gene            complement(1681..2391)
                     /locus_tag="PLQ77_04495"
     CDS             complement(1681..2391)
                     /locus_tag="PLQ77_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPL09969.1"
                     /translation="MNTTSKLMMYCCMVIFVLLMMTPAFAQEGKDAPPPTVVAGQETD
                     SAAVVPEAATKWYVGVIAGGALADDLNIANNAFPGNIEPKAGFAAGVAAGFKLPYGRL
                     EAEFSYLRNQLGKVTADGKEIASSGAISNFALLINGYIDFKNRTAFTPYLSAGMGASN
                     FNIDSMPVKVGGAYVMENANSVVFAYQLGAGVGFAVTDHWTIDLKCRYFATSDPDVAK
                     STLKFRSSNIFLGARYSF"
     gene            complement(2671..2745)
                     /locus_tag="PLQ77_04500"
     tRNA            complement(2671..2745)
                     /locus_tag="PLQ77_04500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2710..2712),aa:Glu,seq:ctc)
     gene            complement(2891..4294)
                     /gene="gltX"
                     /locus_tag="PLQ77_04505"
     CDS             complement(2891..4294)
                     /gene="gltX"
                     /locus_tag="PLQ77_04505"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPL09970.1"
                     /translation="MTAKIRTRFAPSPTGYLHIGGARTALFSWLFARHQGGEFVLRIE
                     DTDQQRSTDESTRAILDAMTWMGLAWDEGPYFQAQRVDLHRQMVQKLLEEGKAYVCTC
                     TPEELEIKRKEALAAGKKPKYDGACRDKGLKKSPGCVVRFRGTDAGVTVVEDLIKGNI
                     VFNNEELDDLIIERGDGYPTYNFAVVIDDAMMNITHVIRGDDHVNNTPRQILMYQALG
                     FEVPKFAHVPMILGSDKTRLSKRHGATSVMAYKEMGYLPEALVNYLVRLSWSHGDQEI
                     FSLKELIEFFSLDAVGKSPAVFNPEKLLWLNAHYIKEASPARLAEEMKPLWPAEARLG
                     DAAFAQKVIADLQPRVKTLVDLAKAAEFYFTDEIVYEAEAAQKFLTPDVSAHLQSIAA
                     AIPGIEDFSKAGIEAFLKSFIEAKGIKFKAIAQPLRVALTGKTVSPGIDEVMVTLGKE
                     RVAKRITAAVDHIAKRQ"
     gene            4459..5001
                     /locus_tag="PLQ77_04510"
     CDS             4459..5001
                     /locus_tag="PLQ77_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPL09971.1"
                     /translation="MSDSLKINLAILPEDGLRLRFSEGNAWFQGCFAGDAPPEFSLVS
                     ADADCLITRSGDTIYVRGDLSAQMSRECSRCLEPATISAGGAFAYTLVPEKTETAQDL
                     ELSAEELETGCYRGDFIDLAPIICEQIVLVAPMKFLCAENCRGLCPHCGVNLNTGSCS
                     CRSETVDDRLAALKKFTVKK"
     gene            5022..5207
                     /gene="rpmF"
                     /locus_tag="PLQ77_04515"
     CDS             5022..5207
                     /gene="rpmF"
                     /locus_tag="PLQ77_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPL09972.1"
                     /translation="MPNPVKRHSKTRRNMRRAHDFLKQPSVSACPQCGEPKMPHRVCP
                     NCGTYKGREVIPAEKMD"
     gene            5307..5543
                     /gene="acpP"
                     /locus_tag="PLQ77_04520"
     CDS             5307..5543
                     /gene="acpP"
                     /locus_tag="PLQ77_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPL09973.1"
                     /translation="MSLEEKVIKLVTEQLDVTPEQCKLEASFIDDLGADSLDLVELIM
                     EMEEAFGVEIADEELEKIRTIQDVVDFLRKKGVN"
     gene            5565..6806
                     /gene="fabF"
                     /locus_tag="PLQ77_04525"
     CDS             5565..6806
                     /gene="fabF"
                     /locus_tag="PLQ77_04525"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPL09974.1"
                     /translation="MKRRVVITGIGAVTPLGNTAAESWANAVAGKSGIGPITRFDASA
                     FASRVAGEIKNFDPLQFVDKKEARRQDKFTVYALAASQMAMDDAALTLGPDIAERVGV
                     IIGSAIGGAATFEEEALVLHSSGPRKISPFSVPAILANLASGHVSIRFGAKGPINCAV
                     TACATGTSAIGDAYKIIAHGDAEAMIAGGVEAAITPLGIAGFCAMRALSLRNDEPEKA
                     SRPFDRGRDGFVIAEGCGVLILEELSFALSRGAKIYAEIIGYGCTSDAYHLAAPPPGH
                     EGAGRSMKAAIRDAGLEPSDIDYINAHGTSTPLNDLYETQAIKALFGEHAKKLLISST
                     KSMTGHMLGGTGAVEAMFAALALQEGVIPPTVNLDDPDDECDLDFVPKVARRRDISTA
                     LSNSFGFGGVNAVLAFRKYTP"
     gene            6831..8081
                     /gene="glyA"
                     /locus_tag="PLQ77_04530"
     CDS             6831..8081
                     /gene="glyA"
                     /locus_tag="PLQ77_04530"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPL09975.1"
                     /translation="MSFLNQVDPEVAQAIQLETRRQAGKLELIASENFVSEAVLEAQG
                     SVMTNKYAEGYPDKRYYGGCEYVDIPEKLAIERCKKLFGCDEVNVQPHSGTQANMAVY
                     FAACSPGDTVLGMNLSHGGHLSHGSPANFSGKFYKIVPYGVSRDTETIDYEELAKIAR
                     ESRPKMIVVGASAYPRIIDFEKFRAVADEVGAVIMADIAHIAGLVAVGLHPSPVPVCE
                     YVTTTTHKTLRGPRGGLIMCQAPCIKVLNSRVFPGMQGGPLMHVIAAKAVALKEALSP
                     EFKTYQQQIVKNAKAMAAQLKDEGFRLVSGGTDNHLMLVDLTAKGVTGKDAQEALDRA
                     AITVNKNGIPFDTLGPMITSGIRIGTPALTTRGMKEEEMRLIASLIAEVIHHIGDERK
                     IQAVAGKVKTLCDRFPLYAGRIQE"
     gene            8130..8624
                     /locus_tag="PLQ77_04535"
     CDS             8130..8624
                     /locus_tag="PLQ77_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPL09976.1"
                     /translation="MSENNEPSRRRPDQVRSRPDWDEYFMDIVELVSRRSTCLRRAVG
                     AGLVRDRRILASGYNGAPSKLQHCLEIGCLRDQLHVPSGERHELCRGLHAEQNAIIQA
                     ALHGVSAKGATLYCTHHPCVICAKMIINAGIARIVIRDSYGDALAADMLREAGISVET
                     LKTL"
     gene            8644..9105
                     /gene="nrdR"
                     /locus_tag="PLQ77_04540"
     CDS             8644..9105
                     /gene="nrdR"
                     /locus_tag="PLQ77_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPL09977.1"
                     /translation="MKCPFCGDAENKVIDSRISKDGKAIRRRRECMGCSRRFTTYEYV
                     EDILPMVVKKDGRREPFDRTKIRSGVRTACEKRPISTEDIEKLVESVEAACQELQGEE
                     IPSSAIGEKVMDELKALDGVAYVRFASVYRQFRDVAEFMSELKDLLSKGKK"
     gene            9105..9740
                     /locus_tag="PLQ77_04545"
     CDS             9105..9740
                     /locus_tag="PLQ77_04545"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPL09978.1"
                     /translation="MFTGIVEGLGKVVRLTVKGADAVLEIDAGIDMAEVSPGDSIAVN
                     GACLTVTSKKGNVFSADVSAESLSRTTLKYLQAGHRVNLEKSLRAGGFLGGHFVLGHV
                     DGAGRVLSRTQKSGSVIFAFESSEALSRHIVEKGSVAIDGVSLTVNRLEKGRFYVNII
                     PHTAAHTTLEGKKEGEWVNIETDILGKYVEKLLQTPRGIDKDFLAEHGFIK"
     gene            9755..10957
                     /locus_tag="PLQ77_04550"
     CDS             9755..10957
                     /locus_tag="PLQ77_04550"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPL09979.1"
                     /translation="MAVSKISEAIEDIRNGKMVILVDDEDRENEGDLCMAAQFVTPEA
                     INFMARLGRGLICLTVNEDIASRLKLNLMVKDNQARFGTAFTVSIEARHGVTTGISAA
                     DRATTILAAVNPAAKAEDIVSPGHVFPIRARKGGVLVRTGQTEGSVDLCRLAGLTPAG
                     VICEVMKDDGTMARMPDLEIFAREHQLKIVTIADLIDYRMQHESLIRRVAEASLPTLY
                     GGDFKIIVYENDVDDMQHIALVKGEIRKEDEVLVRVHSECCTGDIFASARCDCGDQLH
                     RAMEMIEEEGKGVIVYMRQEGRGIGLVNKIKAYALQDEGQDTVEANIALGFKADLRDY
                     GIGAQILADLGVHRMRMMTNNPKKIVGLEGYAIAVTQRVPIEIPPNENNIRYMTTKKK
                     KMGHILEL"
     gene            10983..11453
                     /gene="ribE"
                     /locus_tag="PLQ77_04555"
     CDS             10983..11453
                     /gene="ribE"
                     /locus_tag="PLQ77_04555"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPL09980.1"
                     /translation="MPKIIEGKIVAKGMKFAIAASRFNDFISGRLIDGAVDTLVRAGA
                     EEKDIAIYKVPGAFELPLAAKKLAKSKRFDAVICLGAVIRGATPHFEYVSAEVSKGIA
                     SVGLEAEIPVAFGVLTTDTIEQAIERAGTKSGNKGADAAMAAVEMVDLFKKMNA"
     gene            11489..11959
                     /gene="nusB"
                     /locus_tag="PLQ77_04560"
     CDS             11489..11959
                     /gene="nusB"
                     /locus_tag="PLQ77_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPL09981.1"
                     /translation="MFEDSMQGRRKAREVALQVLYGLNFVNLDVEKAQELFWGNFVAP
                     KSAREFASTLISGTWRHREEIDRLIADCSDNWSLGRMSKVDISILRMAVFEFLHCDDI
                     PPKVSINEAVDLGKTFGSENSGSFINGILDALHLKIQKNHADSSVHPAARPAKA"
     gene            11913..12488
                     /locus_tag="PLQ77_04565"
     CDS             11913..12488
                     /locus_tag="PLQ77_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPL09982.1"
                     /translation="MQIHLSTQPPDRQKHKCLFLGFFEDEKPPRGICGLMDWRLNGLI
                     SREIKQGHILGAFKEQVAIPQPERIDAELLLLFGLGSLADLSYDRIYEAAYAVMAAAG
                     RMKLWEFSFDLPGDGRSALAPAAVLEAMLTGFFDGLSEDIARLTHTKVCLVASPGNLE
                     EIAEGLKRFNENVKHMGSVDFSLLQSHLAPS"
     gene            complement(12582..12989)
                     /locus_tag="PLQ77_04570"
     CDS             complement(12582..12989)
                     /locus_tag="PLQ77_04570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL09983.1"
                     /translation="MIMKKLPARVESLHELLTFIRAEAKKLGFSESAIGRIELVAEEA
                     LVNVFVHGYAGEQGQVEVHCLVSDDPALTVEVHDTGVAFNPLSLADPDVQSDLTKRKI
                     GGMGVFFIRKMTDRVAYRREGNRNILSMTFLNR"
     gene            complement(13008..13343)
                     /locus_tag="PLQ77_04575"
     CDS             complement(13008..13343)
                     /locus_tag="PLQ77_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPL09984.1"
                     /translation="MEIVVANESDVAVFAITGRLDAVSVPQLEEKLSQWFEQPGKKLI
                     FDLEGLDYISSAGLRVFLTTAKKMKAREGKLCMARLRDNVKDVFTISGFIALIPAFDT
                     LGAAKDAVK"
     gene            13534..14841
                     /locus_tag="PLQ77_04580"
     CDS             13534..14841
                     /locus_tag="PLQ77_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPL09985.1"
                     /translation="MIYNEEFETLPREALEALQLKRLQQVASRLYHNVGFYKKSFDRA
                     GVTPESLRTLEDLKRFPFTTKQDLRDNYPFGLFAAPMSNVVRLHASSGTTGASTVVGY
                     TKRDIDTWAELMGRCFVAAGLTKNDVIHNAYGYGLFTGGLGAHYGAEKIGASVVPMSG
                     GNTKRQIMILQDFRPTAICCTPSYALNLAEQGRAMGVDMRSLALRVGVFGAEPWSEQM
                     RAEIEKAMNLKAFNIYGLSEIMGPGVAMECDEARDGMHIFEDHFLVETIDPETGEVLP
                     PGAEGELVFTTLTKEAFPLIRYRTRDISRLNPAPCRCGRTHYRMGRVMGRSDDMLIIR
                     GVNVFPSQIEAVLVGIEGLEPHYQLIVDREGTLDTLEVQVELTEKQFDGADEVKVLQR
                     LENQLKKDMKDYLGITARIKLVEPKTIQRSEGKASRVIDKRKL"
     gene            complement(14935..16761)
                     /locus_tag="PLQ77_04585"
     CDS             complement(14935..16761)
                     /locus_tag="PLQ77_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPL09986.1"
                     /translation="MQSNIAKAFGRTVLIGSGAIGTCLRGTVGIGDEPVELLNLRSPE
                     TVKNLHAAYRDAGSQILVTNTFAANALVLGEAGAADRCDDVNRAGVALAREAGGPEGL
                     VWASVGPLGLGLRLEDYPDAALEEIYRRQCLALTEADALLLETFAEPREAKAAMAAAA
                     ATGLPVIFQIGNTGGGGQRWERVDRLLDEAHRAGVIAVGTNCHHPNEIVRVASYIAAH
                     TNLPVTASANAGHPQIERGAITYEYSPEAFASFALELAAAGVAVIGGCCGTTPEHIRK
                     LSEALGGRPVAARPQPAVFAEPVGPAVRQAGEAANPIRSLLAQKPFIISVEIRAERTR
                     SLEEIVQGAEVVAQAGADMFDVPDNPGATVGRDAMVTAARLQSALDVPSIFHLGVTQS
                     NLMRLHSSLIGGWDLGVRGLIAVTGDAPSMGHLGSLAHRVMDVRSSVELLRLIRSLRE
                     GKIINGEGLADPPDFCAGCAIARPIPAQIKWLKTKIDAGAEFVFSQPVFALDDFHRLR
                     DALAGLQIRFFPGIMPLTSRRNAAFLAGGRIPGIVVSSEVAAGFERYESPEDQRRFGL
                     DRACELAAGIAAEASGLYLIMPFGKSCYEDAVRIVREVRKGK"
BASE COUNT         3710 a         4866 c         4975 g         3391 t
ORIGIN      
        1 aaacaccggg ggtggcgtga aaacaccggg ggtggcgtga aaacaccggg ggtggacgtt
       61 ttgtccctct cccttgaggg gagagggtta gggtgagggt gaaaattaaa gactgcaccc
      121 ccgcccctca gtccccgccc gccaggggcg gggaagtttc cggcggggac attaaatcga
      181 tatttcctat gttcttgaga cacagtctgt aaatgcggga cggacggtgc gatccggaca
      241 ctctttcgct tcattgttaa ttgattaatt gttatgtctc ccgaattgcc ctattttcct
      301 gcttgcattt ttactacttt atgagtatgg gttgattcca acagcgtaca agcggtgaac
      361 gcagcagcgt tttcaaacag acaaatgata gaaaccgact gggtgaatcg tgaaaagtgc
      421 cgcggaatat atggatatcc ccgacggaaa acgcatctgg tttcgggatt tccggcctca
      481 ttttgattcc cttgttttga atcccatcaa aaaatttcct ctgcagaagg atgatatgct
      541 ggtcgctttt gtcttcatgt cctgcagcat cgattatctg gccggtttct ggtggggtga
      601 aaaccgggaa accggcatgg cccggcaggc ctatgtcgga tttatcaacg agtattttct
      661 gccgcgcggc cgctataacg ccaagggcct ttatgacagc ctgcgaaacg gcctggttca
      721 cctgtttacg attaaagaca aaatgtatga gctgacgttc gatgagccgc agaggcatct
      781 tacaacaagt catcacggct acacggtgct ggatgcgggc agtttccgcg ccgatctgct
      841 ggctgcggaa acccgctatc tggatgaggt ggaaaccagc cccccgctgc tggacaaggc
      901 gtttcagcgc tacgcccgtg acgggttcgt gagatgggtt gactgagaaa gggatccttc
      961 tatgaccaaa ctgacagtga tctgcaatga cgaagaaacc gaggcgcggg tgctccacgg
     1021 actgcacaat gatcaggaat tcagcatcag cggggctatt gtcgtccgga acgtgagggc
     1081 ggaagcgcaa aatttcctga actttctgaa caaggcgatc gacaagatgc cggatgtcat
     1141 catcctggac atggccgtcc tgcgcgaggc agccgcgatc gatatgaagc ggctgatgga
     1201 ttacgtgcgc aaattttcct ccgtgcggtt cattattctg ggcaaccgat atcacgagca
     1261 gaacgtgatt gcgatgatgc ggggaggagc gcgcgggttt gtccagaaag atcacctgga
     1321 agaggatatc gccccctccg tccgcgtcgt cacccgcggc gaggtctggc tttcgcccgg
     1381 tctgctgggc cgcgtctgcg gcaagctcct gatggattca cagaaaagcg atatggtcaa
     1441 atctcccacc agcgagcagt tggtgaagct gaataaaatt tcccaccggg aaatggaggt
     1501 tttggagctg gtcagcgaaa gcctgaccaa cgaagaaatc gcgcacagac tttttttaag
     1561 caacaaaacc gtcaagacgc atgtgcgcaa tattttcgag aaaacaggcg tccgcaaccg
     1621 caccgaggcc gccctgcttt ttacacgcta ccatcaggat atcgaaaacc atttaaagca
     1681 ttaaaacgag tatcttgctc cgaggaagat attgctgctt ctgaatttca gcgtggactt
     1741 ggccacgtcg ggatcggaag ttgcaaaata ccggcatttc aaatcgatgg tccagtggtc
     1801 cgtcacggca aagccgacgc ccgcgcccaa ctggtaggca aagacgacgc tgttcgcgtt
     1861 ttccatcacg taggcgccgc ccactttcac cggcatggaa tcgatgttga aattggaagc
     1921 gcccatgccc gcgctcaggt agggtgtaaa ggcggtcctg tttttgaaat caatgtagcc
     1981 gttgatcaac aacgcaaaat tggaaatcgc gcccgatgag gcaatctctt ttccatcggc
     2041 ggtgactttg cccaactgat tgcgcaggta ggagaactcc gcctccaacc ggccataggg
     2101 taatttaaaa ccagcggcca cgcccgccgc gaagcctgcc ttgggctcaa tgtttcccgg
     2161 aaaagcgtta ttggcgatgt tgagatcatc ggccagcgct ccgcccgcaa tgacgccgac
     2221 gtaccacttg gtcgcggctt ccgggacgac cgccgcggag tcggtttcct ggcccgcaac
     2281 aaccgttggc ggcggcgcgt cttttccttc ctgggcaaaa gccggcgtca tcatcaataa
     2341 aacaaaaatc accatgcagc aatacatcat cagttttgac gttgtgttca ccgtagcctc
     2401 cttttgtaat tggggtcaaa tctttattct tgacatttcg accggaaaat cagggacagc
     2461 gcctctttat cagcaatgag caatgagtga cgggtctgca gggaacggcc gcgaccattc
     2521 cgcgccggcg cccctgaagt cccctggtag cgcacctgaa aattcgccct gtgtttctct
     2581 cttttgttag aaaacctgtt ccgggaacag gatgaaatca gagagagatc aggcaaatgt
     2641 caagaataaa gatttgaccc caaatgtttt tggtagtccc acgggggttt gaaccccggt
     2701 ttccggcgtg agaggccagc gtcctaacca ctagacgatg ggaccatgaa attctttcta
     2761 tccgggtgcc gccaaacgac aatccgccaa aagcagttga aagtcgagcg tgtttaccta
     2821 gtcaaaccgg cgcgaaatgt caaggcaaaa ttgccgcctc atgccgaaac tctgcctgtc
     2881 ttcggccggt tcactgccgc tttgcgatgt ggtccacggc ggcggtgatt ctttttgcca
     2941 cccgctcttt ccccagcgtg accatgactt cgtcgatgcc gggactgacg gtttttccgg
     3001 tgagcgcgac ccgaaggggc tgggcgatcg ccttgaactt gatgcctttg gcctcgatga
     3061 agcttttcaa aaacgcttcg atgcctgcct tgctgaaatc ctcaatgccc ggaatggccg
     3121 cggcgatgga ctgcaagtgc gccgatacgt cgggggtcag aaacttctgc gcggcttccg
     3181 cctcgtaaac gatctcgtcg gtaaagtaga attccgcggc tttcgccaga tccaccagcg
     3241 ttttgactct cggctgcagg tcggcaatca ccttttgcgc aaacgcggcg tcgcccaaac
     3301 gggcttcggc gggccacagg ggcttcattt cttccgccag ccgcgccggg cttgcctcct
     3361 tgatatagtg ggcgttgagc cacagcagtt tttcgggatt gaaaacagcg ggcgatttgc
     3421 cgaccgcatc gagcgaaaag aactcgatca gctccttcag ggaaaaaatt tcctggtctc
     3481 cgtgcgacca gctcaggcgc accaggtagt tgacgagcgc ttccggcaga tagcccattt
     3541 ccttgtaggc catgacggag gtggccccgt gtcgcttgct caggcgggtc ttgtcggatc
     3601 ccagaatcat cggaacatgg gcgaacttcg gaacctcaaa gcccagcgcc tgatacatca
     3661 gaatctggcg cggggtgttg ttgacgtggt cgtcgccgcg aatgacatgc gtgatgttca
     3721 tcatcgcgtc gtcgatgacc acggcgaaat tatacgtggg atacccgtcg ccgcgctcga
     3781 tgatcagatc atccagttct tcgttgttaa atacgatatt ccccttgatg aggtcttcca
     3841 cgacggtgac gccggcatcc gtgccgcgga agcggacgac gcagcccggc gactttttca
     3901 ggcctttgtc ccggcaggcg ccgtcgtatt tgggtttttt cccggccgcg agcgcctctt
     3961 ttctttttat ctccagttct tcgggcgtgc aggtgcagac gtaggccttg ccttcctcca
     4021 aaagcttttg caccatttgc cggtgcaggt ccacccgctg ggcctgaaaa tacggtcctt
     4081 catcccaggc aaggcccatc caggtcatgg cgtccagaat cgcccgggtg gattcgtccg
     4141 tggagcgctg ctgatccgtg tcttcaattc tcaatacaaa ttcaccgccc tggtgccggg
     4201 caaaaagcca gctgaaaagg gcggttctgg ctcctccgat gtgcagatag ccggtcgggg
     4261 acggggcaaa gcgggtgcga attttggcgg tcatcctgtt cctcttgatt tttgcgctgt
     4321 tatagggcgg cggccaatca tttgtcaagc ggcttttgcc tttcggcaag caaattatgc
     4381 ttgacaatac aatcatttta tcattaactt ccccgctcaa tcggagttgg gcttattaca
     4441 acagggctgc aagattcaat gtctgattct ttgaaaatta atctggccat tctcccggaa
     4501 gatggcctgc ggttgagatt ttccgaaggc aacgcctggt ttcagggatg ttttgcaggc
     4561 gatgcgccgc cggaattctc gctggtcagc gcggatgcgg actgtttgat cacccgctcg
     4621 ggcgatacga tttatgtccg cggggacctc agtgcgcaaa tgagccggga atgcagccgc
     4681 tgtctggagc ccgcgacgat ttccgcgggc ggagcattcg cctacacgct ggtcccggaa
     4741 aagacggaga cggcgcaaga tctggaactt tccgcggaag agctggaaac cggctgctat
     4801 cgcggtgatt ttatcgatct ggccccgatc atctgcgaac agattgttct ggtggcgccg
     4861 atgaagtttc tctgcgccga aaattgcaga gggctatgcc cgcattgcgg cgtcaattta
     4921 aataccggtt cgtgcagctg ccgttcggaa acggtcgatg accgcctggc ggcgctgaaa
     4981 aagtttacag taaaaaaata attcaatcat gaggatacgc catgccaaac ccagtaaaaa
     5041 gacattctaa aacaagacgc aacatgcgcc gggcgcacga ttttctgaaa cagccttccg
     5101 tgtcggcttg cccgcagtgc ggggagccga aaatgcccca ccgggtctgc ccgaattgcg
     5161 gcacgtacaa gggcagggaa gtgattccgg cggaaaagat ggactaatgc cgtccgggca
     5221 ggcgcccggc ttttgccggc cgcggatgac gggcggggaa gcgccctgaa tcgttttaac
     5281 ctaatcttcg gctcgaggag ggatgcatgt cattagaaga aaaagtcata aaactcgtca
     5341 cggaacagct ggacgtcaca ccggagcagt gcaagctgga agcctctttc attgatgatc
     5401 tgggcgctga ttctctggat ctggtggagc tgatcatgga aatggaagaa gcgttcggtg
     5461 tggaaatcgc cgacgaagag ctggaaaaaa tccggacgat tcaggatgtc gtcgactttc
     5521 tccggaaaaa gggcgtcaat taattcgcgg acaggggttt gtcgatgaaa aggcgcgttg
     5581 tgatcacagg cattggcgcc gtgactccgc tgggcaacac cgcggcggaa tcctgggcga
     5641 acgccgtggc gggaaaatcc ggcatcggcc ccatcacccg gtttgacgca tccgctttcg
     5701 cttcccgcgt cgcgggcgaa atcaaaaatt ttgatccgct gcagttcgtc gataaaaaag
     5761 aagcccgccg tcaggataaa ttcaccgttt acgcgctcgc cgcgtcccag atggcgatgg
     5821 acgacgcggc gctgacgctc ggcccggaca ttgccgagcg ggtcggcgtg atcatcggct
     5881 ccgccatcgg aggggccgcc acctttgaag aagaagccct cgtgcttcat tcctcgggcc
     5941 cccgcaaaat atcccccttc tccgttccgg ccattctggc caatctggcc tccggccatg
     6001 tttccattcg cttcggcgcc aaaggcccca tcaattgcgc cgtaacggcc tgcgccaccg
     6061 gcacgtcggc gattggcgac gcctacaaaa tcatcgctca cggcgacgcg gaggccatga
     6121 ttgccggcgg cgttgaggct gcgatcacgc cgctggggat cgcgggattc tgcgccatgc
     6181 gcgcgctgtc cctgcgcaac gacgaaccgg aaaaagccag ccgcccgttc gacagggggc
     6241 gcgacggctt tgtcattgcg gaaggctgcg gcgttttgat tctggaagaa ctgtctttcg
     6301 ccctgagccg gggcgcaaag atctacgcgg agatcatcgg atacggctgc acgtcggacg
     6361 cctatcacct cgccgctccg cctcccggac atgaaggcgc gggccgcagc atgaaagcgg
     6421 cgatccgcga cgcaggcctg gagccctccg atatcgacta catcaacgcc cacggcacgt
     6481 ccaccccctt aaacgatctt tacgaaacgc aggcgatcaa ggcgcttttc ggcgagcacg
     6541 caaagaaact gctgatcagt tcgaccaaat cgatgacggg ccacatgctg ggaggcacgg
     6601 gcgccgtgga ggcgatgttt gccgccctgg cgcttcagga gggcgtcatt ccgcccaccg
     6661 tcaatctgga cgatcccgac gacgagtgcg atctggattt tgtgccgaag gtcgccaggc
     6721 gccgggacat ttccaccgcc ctgtccaaca gctttggatt cggcggcgtc aatgccgttc
     6781 tcgcattcag aaaatatacg ccatagtttt tggaaaagga gttttcattc atgtcttttt
     6841 tgaatcaggt cgatccggaa gtcgcacagg ccatccagtt ggaaaccaga cgccaggcgg
     6901 gaaagctcga gctcatcgcc tcggaaaatt ttgtcagcga agcggtcctg gaagcccagg
     6961 gttcggtgat gacgaacaag tacgccgaag ggtatcccga caaacgctat tacggcggct
     7021 gtgaatacgt ggacattccc gaaaagctgg ccatcgagcg ctgcaaaaag cttttcggct
     7081 gcgatgaagt caacgtccag ccccattccg gcacgcaggc caacatggcc gtttattttg
     7141 ccgcctgctc tcccggggac acggtgctgg gaatgaatct gtcgcacggc ggacatctct
     7201 cgcacggctc tccggcgaat ttttccggca agttctacaa aatcgtcccc tacggcgtca
     7261 gccgcgacac ggaaaccatc gactatgaag agctggcgaa aatcgccagg gaaagcaggc
     7321 ccaagatgat cgtggtgggc gccagcgcct acccgcggat catcgatttc gaaaagtttc
     7381 gcgccgtggc cgatgaagtc ggcgcggtca tcatggcgga catcgcgcat atcgccggcc
     7441 tggtcgccgt cggcctgcat ccgtcgccgg ttcccgtttg cgagtacgtc accacgacca
     7501 cgcacaagac cctgcgcgga ccgcgcggcg gactcatcat gtgtcaagcc ccctgcatca
     7561 aggttctcaa cagccgcgtt ttcccgggaa tgcagggcgg tccgctgatg cacgtcatcg
     7621 ccgccaaagc cgtggcgctc aaggaggctc tgtcgccgga attcaaaacc tatcagcagc
     7681 agattgtgaa aaacgctaag gccatggcgg cgcagctcaa agatgaaggc ttccgcctgg
     7741 tgtccggcgg cacggacaat catctgatgc tggtcgatct gaccgccaag ggcgtaaccg
     7801 gcaaagacgc gcaggaggcg ctggaccgcg ccgccatcac cgtcaataaa aacggcattc
     7861 cctttgacac gctgggaccg atgatcacca gcggcattcg aatcggcacg ccggcgctca
     7921 cgacccgcgg catgaaggaa gaggaaatgc gactgatcgc ctccctgatc gccgaggtga
     7981 tccatcacat cggcgacgag cggaagattc aggccgtcgc cggaaaagta aaaacgcttt
     8041 gcgaccggtt tccgctctac gcgggaagga ttcaggaata aaaccagcat ccggcagggc
     8101 gcaggctgcc ggccagaaag caaaatgaca tgtcggaaaa caacgagccg tcgcgccgtc
     8161 ggccggatca ggtccgctcc cgtcccgact gggacgagta tttcatggac atcgtcgagc
     8221 tggtttcaag gcgcagcacc tgcctgagaa gggccgtcgg ggccggactg gtccgcgaca
     8281 ggagaattct ggccagcggc tacaacggcg ctccttccaa actgcagcac tgcctcgaga
     8341 tcggctgcct gcgcgatcag cttcacgtgc cgtccggcga acgccacgag ttgtgccgcg
     8401 gtctgcacgc ggagcaaaac gcgatcattc aggccgcgct gcacggcgtc agcgcgaagg
     8461 gcgccacgct gtactgcacg catcatccct gcgtgatctg cgccaagatg atcatcaacg
     8521 cggggatcgc gcgcatcgta atccgcgaca gctacggcga cgccctggcc gcggacatgc
     8581 tgcgggaagc cggcatcagc gttgaaaccc tgaagacctt gtaaaggacg gcacgcgaac
     8641 gtcatgaaat gtcctttttg cggcgacgcg gaaaacaaag tcatcgattc acggatcagc
     8701 aaggacggca aggccatccg ccgccgccgc gagtgcatgg gatgctcccg ccgcttcacg
     8761 acgtatgaat acgtggaaga cattctgccg atggtcgtga agaaagacgg gcggcgcgag
     8821 ccgttcgacc ggaccaaaat ccgaagcggc gtgcggacgg cgtgcgaaaa gcggccgatc
     8881 agcacggagg acattgagaa gctggtggag agcgtggagg cggcctgcca ggagctgcag
     8941 ggcgaggaaa tcccctcttc ggccatcggc gaaaaggtga tggacgaact caaagccctg
     9001 gacggcgtcg cgtacgtccg gtttgcctcg gtgtaccggc agtttcgcga cgtggcggag
     9061 ttcatgtccg aattgaagga cctgttgagc aaaggcaaga aataatgttt accggcatcg
     9121 tggaaggatt gggaaaagtc gtccgcctca ccgtaaaggg ggcggatgcc gttttggaaa
     9181 tcgacgcggg catcgacatg gccgaggtgt cccccggcga cagcatcgcg gtcaacggcg
     9241 cctgcctgac cgtcacatcc aaaaaaggga acgtcttttc cgccgatgtt tccgccgaat
     9301 cgctttcgcg aacgacgctg aaatacctgc aggcggggca ccgcgtcaat ctggaaaagt
     9361 ccctgcgcgc cggcggattt ctgggcggcc actttgtgct gggccacgtg gacggcgccg
     9421 gccgcgtttt gtccagaacg cagaagtccg gctccgtgat ctttgcgttc gaatcctccg
     9481 aagcgctgtc gcgccatatc gtggagaagg gatcggtggc catcgacggc gtcagcctga
     9541 ccgtgaacag gcttgaaaaa ggccggtttt atgttaatat cattccgcat acggccgccc
     9601 acacgacgct tgaggggaaa aaagagggcg aatgggtcaa catcgagacg gatattctgg
     9661 gcaagtatgt ggaaaagtta ttgcaaaccc cgcggggaat cgataaggat tttttagccg
     9721 aacacggctt tatcaaataa acgaaggaca aaacatggca gtcagtaaaa tttcggaagc
     9781 aatcgaagac atccgcaacg ggaagatggt cattctcgtg gatgatgaag accgggaaaa
     9841 cgagggcgat ctctgcatgg cggcgcagtt tgtcacgccg gaagcgatca atttcatggc
     9901 caggctgggg cgcggattga tttgcctgac cgtcaatgaa gacatcgcct cgcgcctgaa
     9961 gctcaacctg atggtcaagg acaatcaggc gcgcttcggc acggccttta ccgtctccat
    10021 cgaagcgcgc cacggcgtga ccaccggcat ttccgcggcg gaccgcgcca ccacgattct
    10081 ggccgcggtc aatcccgccg ccaaagccga ggatatcgtc agccccggcc acgtgttccc
    10141 gatccgcgcg cgcaagggcg gcgtcctggt gcgcaccggc cagaccgagg gctcggtgga
    10201 cctgtgccgc ctggccggat tgacgccggc gggcgtgatc tgcgaagtca tgaaggacga
    10261 cggcacgatg gcgcgaatgc cggacctgga aatcttcgcc cgcgaacacc agcttaaaat
    10321 cgtgacgatc gccgatctca tcgattaccg aatgcagcat gagtcgctga tccgccgcgt
    10381 ggccgaagcc tcgctgccca cgctgtacgg cggcgatttc aaaatcatcg tatatgaaaa
    10441 cgacgtggac gacatgcagc acatcgcgct ggtcaagggg gaaatccgca aggaagacga
    10501 agtgctggtg cgcgtgcatt cggaatgctg caccggcgac attttcgctt cggcccgctg
    10561 cgactgcggc gatcagctgc accgcgcgat ggaaatgatt gaggaggaag gaaaaggcgt
    10621 catcgtttat atgcgccagg aaggacgggg catcggtctg gtcaacaaga tcaaagcgta
    10681 tgcgctgcag gatgaaggcc aggacaccgt cgaagccaat atcgcgctgg gcttcaaggc
    10741 cgatctgcgg gattacggca tcggcgcgca gattctggcg gatctgggcg tgcacaggat
    10801 gcggatgatg accaacaatc ccaagaagat tgtcggcctg gaagggtacg ccattgccgt
    10861 gacccagcgg gtcccgattg agattcctcc caacgaaaac aatatccggt atatgaccac
    10921 gaagaagaaa aagatgggcc atattctgga attataatca accaaaggat ggtgctgaaa
    10981 tcatgccgaa gatcattgaa ggaaaaattg tcgccaaagg gatgaaattc gcgatcgccg
    11041 cgagccggtt caacgatttc atcagcggac gcctcatcga cggcgcggtg gacacactgg
    11101 tccgggccgg agcggaggag aaagacatcg cgatttacaa ggttcccggc gcgtttgaac
    11161 tgccgctggc cgcgaaaaaa ctggccaaaa gcaagcgctt tgacgccgtg atctgcctgg
    11221 gcgcggtgat ccgcggcgcg acgccgcact ttgaatatgt cagcgcggaa gtgtccaagg
    11281 ggatcgccag cgtcggcctg gaagcggaaa ttccggtggc cttcggcgtt ctgacgacgg
    11341 acaccatcga acaggccatt gaacgggcgg gcaccaagtc cggcaacaag ggtgcggacg
    11401 ccgcgatggc ggccgtcgaa atggtggacc tgttcaagaa gatgaacgcc tgaaaaacgg
    11461 cgggaccgcg ccggcggaat ccggcgcgat gtttgaggat tcgatgcagg gacggagaaa
    11521 ggcgcgcgaa gtcgccttgc aggttcttta cggtttgaat tttgtcaatc tcgatgtgga
    11581 gaaggcccag gaacttttct gggggaattt tgtcgccccc aaatcggcca gggaattcgc
    11641 gtccacgctc atttccggca cctggcgcca tcgggaggag atcgaccggc tcatcgccga
    11701 ttgctcggac aactggtcgc tgggaaggat gtccaaggtg gacatcagca tcctgcgcat
    11761 ggccgttttt gaatttcttc actgcgacga cattccgccc aaagtcagca tcaatgaagc
    11821 ggtcgatctg ggaaaaacct tcggatcgga aaattccggc tcgtttatca acggcatcct
    11881 ggatgccctc catttgaaaa ttcagaaaaa ccatgcagat tcatctgtcc acccagccgc
    11941 ccgaccggca aaagcataaa tgcctgtttt tgggtttttt cgaagacgaa aagcccccgc
    12001 gcggcatctg cgggctgatg gactggcgcc tcaacggcct gatttcccgg gaaatcaaac
    12061 aggggcatat tctaggcgcg ttcaaggagc aggtggcgat tccccagccg gagcgaattg
    12121 acgcggagct gctgcttctt ttcgggctgg gcagccttgc cgatctttct tatgatcgga
    12181 tttacgaggc ggcctatgcg gtgatggccg cggcgggcag aatgaagttg tgggaatttt
    12241 cgtttgacct gcccggcgac ggccgctcgg cgctcgcgcc ggccgccgtt ctggaagcca
    12301 tgctcaccgg ttttttcgac ggcctgtcgg aagacatcgc gaggctgacc cacacgaagg
    12361 tctgcctggt ggcgtccccg gggaatctgg aagaaatcgc cgaaggactg aagcgattca
    12421 acgagaatgt aaagcacatg gggtcggttg atttcagcct gctgcagtcg cacctcgccc
    12481 cctcctgatc cccgcaacgc catggaacct cccggcgcaa atcttgcgga ggccaggcgt
    12541 ccctcctgaa gaatgaatcg gaaagagaag atccgccgga ttcagcggtt gagaaaagtc
    12601 atggataaaa tattgcggtt tccttcgcgg cgataggcca cccggtcggt cattttgcgg
    12661 atgaagaaca cgcccatgcc gccgattttg cgtttcgtca gatcagattg aacgtccggg
    12721 tccgccagag acaggggatt gaaggcgacg cccgtgtcat gcacctctac cgtcaacgcc
    12781 ggatcgtccg aaaccaggca atgcacttcc acctgtccct gctctccggc gtagccgtgc
    12841 acgaacacgt tgacgagcgc ctcttccgca accagctcga tgcggccgat ggcgctttcg
    12901 gaaaacccca gtttttttgc ctcggcccga atgaacgtca gcagctcatg caggctttcc
    12961 acccgggcgg gcaatttttt catgatcata ggcgaatccg gccaacgcta ctttacggca
    13021 tctttagccg ctcccagagt gtcgaatgcg ggaatcagcg cgatgaaacc cgaaatggta
    13081 aaaacgtctt tcacattgtc ccgaagcctg gccatgcaca atttcccctc acgcgccttc
    13141 attttcttgg ccgtggtcag aaaaaccctc aggccggcgc tgctgatgta atcgagccct
    13201 tccaggtcga atatcagctt tttccccggc tgctcaaacc actggctgag cttctcttca
    13261 agctgcggaa cggaaacggc atccagtctt ccggtgatgg caaaaaccgc gacgtctgac
    13321 tcattggcaa cgacgatttc cataggtctg ctccttgttg tggtggctcg aacctgaaga
    13381 agcattcgga cggcgcgagt atagaaaaga aaaccggctt ctgtcaataa gttttaaatc
    13441 ttaaatcttc attacttaaa ccttttcttt ttaagtccgg tttgtgctat aggaagcgca
    13501 gaatttttca tagccgaagg agaaccgaaa cccatgatct acaatgaaga atttgaaacc
    13561 ctgccccggg aggcgctgga agcgctgcag ctcaaaaggc ttcagcaggt cgcctcgcgg
    13621 ctctaccata acgtcggttt ttacaaaaaa tcctttgacc gggcgggggt gacgccggag
    13681 agcctgagaa ccctcgagga tctgaagcgt tttcctttca ccaccaagca ggatttgcgg
    13741 gacaactatc ccttcgggct tttcgccgcg ccgatgagca atgtcgtgcg cctgcacgcc
    13801 tcgtccggca ccaccggcgc gtccaccgtg gtcggctaca cgaaacgcga catcgacacc
    13861 tgggcggagc tgatggggcg ctgctttgtg gccgcgggcc tcaccaaaaa cgacgtcatc
    13921 cacaacgcct acggctatgg cctcttcacc gggggactgg gcgctcatta cggcgcggaa
    13981 aaaatcggcg ccagcgtcgt gccgatgtcc gggggcaaca ccaaacgcca gatcatgatt
    14041 ctgcaggact ttcgtcccac cgccatctgc tgcacgcctt cctacgcgct gaacctggcg
    14101 gagcagggcc gcgccatggg cgtggacatg cgctctctgg cgcttcgcgt cggcgttttc
    14161 ggcgcggagc cctggagcga acaaatgcgg gccgagatcg aaaaagcgat gaacctaaag
    14221 gccttcaaca tctacggcct gtccgaaatc atgggtccgg gcgtggccat ggaatgcgac
    14281 gaggcgcgcg acggcatgca catctttgaa gatcattttc tcgtggagac catcgatccc
    14341 gaaacgggag aagtcctgcc gcccggcgcc gaaggggaac tggttttcac cacgctgacc
    14401 aaagaggcct tcccgctcat ccgctaccgg acgcgggaca tttcccgcct gaacccggcg
    14461 ccctgccgct gcgggcggac gcattaccgc atggggcgcg tcatgggcag aagcgacgat
    14521 atgctgatca tccgcggcgt caatgttttc ccctcgcaga ttgaagccgt gctggtcggc
    14581 atcgaaggtc tggagccgca ctaccagttg atcgtggacc gggaaggaac gctggacacg
    14641 ctggaagttc aggtggaatt gacggaaaag caattcgacg gcgccgatga agtcaaggtg
    14701 ctgcagcgtc tggagaatca actgaaaaag gacatgaagg attatctggg catcaccgcc
    14761 aggatcaaac tggtcgagcc caaaaccatt cagcgctccg aaggcaaggc cagccgcgtg
    14821 atcgataaga gaaaattgtg aagagcgcat gacacatagc tgacagctca cggctcatcg
    14881 tggatggtga atagctgacc gtgacaccat ccactatcca ctatccacca tccactattt
    14941 tcccttcctc acctctcgga cgatgcggac ggcgtcttcg tagcagcttt tgccgaacgg
    15001 catgatgaga taaaggccgg aagcctccgc ggcaatcccc gctgcaagct cgcaggctcg
    15061 gtccaggccg aagcggcgct gatcttcagg cgactcgtac cgttcaaaac ccgccgcgac
    15121 ttctgaagaa acaactatgc ccggaatccg tccgccggcc agaaaagctg cgttgcgccg
    15181 gctcgtaagc ggcataatgc cggggaaaaa gcggatctgc agccccgcaa gcgcgtcgcg
    15241 cagtctgtgg aagtcgtcaa gcgcaaagac cggctgggaa aaaacaaact cggcgccggc
    15301 gtcgatcttg gttttgagcc acttgatctg cgcgggaatg ggccgggcaa tcgcgcagcc
    15361 ggcgcaaaag tccggcggat cggccaggcc ctcgccgttg atgattttgc cttcgcgcag
    15421 ggagcggatc agccgcagga gctccacgga gctgcgcacg tccatcacgc ggtgggcaag
    15481 actccccaga tgtcccatgg aaggcgcgtc gcccgtcacc gcgataagcc cccgcactcc
    15541 cagatcccag ccgccgatga gcgatgagtg caggcgcatc agattggact gcgtcacccc
    15601 gagatggaag atggagggaa catccagcgc ggactgaagg cgggcggcgg tcaccatcgc
    15661 atcgcgcccg accgtcgcgc cgggattgtc cggcacgtcg aacatatccg ccccggcttg
    15721 agccacgacc tcggcaccct gcacgatttc ttcgagagac cgcgtgcgct cggcacggat
    15781 ctccacactg atgataaacg gcttttgcgc cagcagcgag cgaatgggat tggccgcttc
    15841 gcctgcctgt ctgacggcgg ggccgaccgg ttcggcgaaa acggcaggct gcggacgcgc
    15901 cgcaaccggc cggccgccca atgcttcgga tagtttgcga atgtgttccg gcgtcgtgcc
    15961 gcagcagccg ccgatgaccg cgacgcctgc ggcggccagc tcaagcgcaa atgaggcaaa
    16021 tgcctccggc gaatattcgt aggtgatagc gccgcgctca atttgcggat gtccggcgtt
    16081 ggccgacgcg gtgacgggca gattggtatg cgcggcaatg taggaggcga cacggacgat
    16141 ttcattcgga tggtggcagt tggttcccac ggcaatgacg ccggcccggt gcgcctcgtc
    16201 aagcagacgg tccactcgtt cccatctctg ccctcccccg ccggtgttgc cgatctgaaa
    16261 aatgacgggc aggcccgtcg cggcggccgc ggccatggcg gctttggctt cgcgcggctc
    16321 ggcaaacgtc tccaggagca gcgcatcggc ttcggtgaga gcaaggcact gtctccgata
    16381 gatctcctcc agcgccgcgt ccggataatc ctccaggcga agccccaggc ccagaggccc
    16441 gaccgaagcc cagaccaggc cttccggacc gcctgcctcc ctcgccagcg ccacgcccgc
    16501 gcggttgacg tcgtcgcacc gatcggctgc gcccgcctcg cccagaacca gagcgtttgc
    16561 cgcaaacgtg ttggtcacca gaatctgtga tcccgcatcc cggtaagccg cgtgcaggtt
    16621 tttgacggtt tcggggcttc tgagattgag aagctccacc ggctcatcgc cgattccaac
    16681 cgtcccgcgc aggcaggtgc cgattgcccc gctgccgatc agcacggtgc gtccgaaggc
    16741 ctttgcaata tttgattgca tatccatctc cttaccgggg gattgcaggc cacaattgct
    16801 gccggttcgg tcctctatat atcacatcag tgaaattttt gaatggtgaa ggcggatgaa
    16861 acgattatct ttatccggca ccgatccgca ggacacccgc acaatactga ccgacttatc
    16921 tgtaaactta aatacttaag gg
//
LOCUS       DAOPAF010000030        16921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3454_length_16921_cov_39.673307, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000030 DAOPAF010000000
VERSION     DAOPAF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16921
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_16921_cov_39.673307"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLQ77_04590"
     CDS             complement(<1..454)
                     /locus_tag="PLQ77_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPL09987.1"
                     /translation="MEIHFLLFVLLLAATAVISFAYAIWRGRNDQQILRSILQSSPIP
                     TFVISTNRKILYWNRALEGLSGIRAEEVLGTDQQWRAFYSSPRSCMADLIISKKIDHS
                     YSLYAGKVAKSKILKYAYEVTEFFPDLGEKGKWFRITATSIRDSSGKTF"
     gene            633..1736
                     /gene="mnmA"
                     /locus_tag="PLQ77_04595"
     CDS             633..1736
                     /gene="mnmA"
                     /locus_tag="PLQ77_04595"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPL09988.1"
                     /translation="MEKGKLLFERRGMQGGAGRKILVAMSGGVDSSVAALLLREAGHE
                     IAGATMLLGVHRPGDAPGRFTDESAADAQRVCDQLGIPHRTFDFADLMENRVIARFIR
                     EYQSGRTPNPCVDCNRHLKFGALFAKARSLGFDCLATGHYARIVKRQSRWALMRPKDE
                     SKDQTYFLYPIPAGDLPSILFPLGALTKTEVRGMARRAALQTALREDSQDLCFVTAGN
                     YRQLFEDRRLPVVPGEIVNPAGQVLGRHRGIVSYTIGQRSGLGISAKTPLYVLGFDPA
                     KNRVVVGKREELRSSGCIAGDLNMLADRFPEDVEAKIRYRKRPVRCRVIREGDRMKVV
                     FEQAQEAVTPGQSVVFYAADEVLGGGVIEEVIR"
     gene            1829..3166
                     /locus_tag="PLQ77_04600"
     CDS             1829..3166
                     /locus_tag="PLQ77_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPL09989.1"
                     /translation="MKTTCRGLRVFIFALSLMLCPAAVSAQALTLEDSIAVALQNSHV
                     LNIAKEGVKGAEAQKKEALTGFLPKFSTTYSYTRLNEDPNFYFPGFAGPPVIQGGPMT
                     TGTINNYNWGIEARQPLFAGGGILANYQAFKIAEDTALVEQTAKSLDVVQDVKIAYFN
                     ILRTQRLMETARQQVDMLAAHCDVAENFYRVGLIPKNDMLHAEVELANGRQTLVKAKN
                     GVESTKARLNTILKREVWTPLEVVDILDYQPLRQSFEECLAVARQRRPELKMSELRVE
                     QAGKMVRAAQSEFFPAVNLVGNYGRFGDTASLSGTQYKDTESWQVMAVASWNFWEWGK
                     TKYRVDAGKARENQALDQSRELNDQIALEIKNAFLALQEAESQIAVSKKVIEQAEENF
                     RIAEARYKERVARSTEVLDAQTLLTRAKAEYANALADYNIAHARLQRAMGVVR"
     gene            3226..4518
                     /locus_tag="PLQ77_04605"
     CDS             3226..4518
                     /locus_tag="PLQ77_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPL09990.1"
                     /translation="MMILWTALLLCSPTAFAQEYSLRDLYAEAVKNSEKIKYAEENLY
                     IAKMGKEKAWAVLMPRVTAFGTYNRFSEKKYGDPPAYGNVLIQPNESGNWGVRADESF
                     SLSIRELDALKISGQLISKSEFDLESTKSDFIFFVATAYYDALKAKKALEIAAANVER
                     LTQYRYFVEKRLKVGEVTRTALLRADGELSGAQSDYLRATNAFQLARAALVRVTGIEE
                     NFRLREEAPDFSAVDAFDRLRQTAFQMRPDLKSYDVQIQVSERQVKYARGAFWPYVGF
                     FAIYNGADQNPATSTLNRESVLAGVSLNFPFFEGGLRVAELKEARSRERQARLAYDDL
                     KKNVDIEIRGAWLDLETQRGTLKFLEDQLVFAHDNYNAVLRQYQNGLATSLDVMDANT
                     LLLTSERNTVDALYGCQLAHLRVRKSSGTLLRFVQEGY"
     gene            4557..5663
                     /locus_tag="PLQ77_04610"
     CDS             4557..5663
                     /locus_tag="PLQ77_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL09991.1"
                     /translation="MRFLQYQNSGGQAARRLSALILLTLAVLTLASCQREKAAEPERL
                     VNVRIWPAETKRVEPYLETTGTLKAFEEVIVSSEVDGILRRIEVDEGSPVHAGMLLAE
                     INDTDYRLDRKRSDAALKQGRASLDNARAEYKRKEALYREELITKQQFDDVSTRVALA
                     EAELERIRATLSISQERLARTKIHSPLSGAVKEKKVSAGDYVRNGTPLFQLIRIHPLK
                     LNFTVSEKDVAALKIGQEVSFTVDAFSGKTFRGRVNLLYPNVEERTRTLQAEAVVPNA
                     DHLLKPGYFARVQIYTQAARDAVLIPVTALLYDGPVIRVFVVRGNQAQERIIKTGNKY
                     GEGVEILEGLKEKEQVVVVGQNNLSDGVKVHVAR"
     gene            5650..8775
                     /locus_tag="PLQ77_04615"
     CDS             5650..8775
                     /locus_tag="PLQ77_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPL09992.1"
                     /translation="MWLADTSVKRPVFATMVIMALVVLGVVSYPSIGVDLFPKIDFPI
                     VTITTTLKGASPDVMDVDVTDRIEGAVNTINGVKTITSTSYESMSRTTIEFVLERDID
                     QAVQDVREKVSGIRNKLPQDIEEPRIAKVDPDAQPILFLNLSGNRSVRDLSMYADEVL
                     KEQLQRISGVGDVIFYGLRLRQVRIWLDAAKMQAYQVAPSDVVLALKRENIELPGGRI
                     ETRTKEYTVRIKGEFAKIPDFNDLIISYYKGAPVRVRDIGRAEDGMEEKRSLSRFNRI
                     PAVGMAIQKQSGTNTVEVADRVKAEVAKINKTLPPGMKVNTAVDQSVFIVRSIDEVQF
                     HLIIGSIFAILAVFIFLKNIRTTLISAVALPVSVISTFALINAFGFTFNNMTMLALSL
                     SVGLLIDDAIIVIENIYRHVEEGMEPREAATFATSEIGLAVMATTFAIVGIFLPVAFM
                     KGIIGRFFLQFALTIIFAIMVSLLVSFTLTPMMASIFLKPHKKSIPNPLAAGGGGGGN
                     VQKPIWTRIGDRMEYFYAKLESSYRTVLEWTLGWRKTVLVCALMIFLLSLGLTAFIGK
                     EFNPPEDQGVFLIRMEAPIDYSVLQIEQYLGKTEAMMQEIPEIKSVFYVQGYGGYPNR
                     AMMMLNLTPKAERKRSQEDIKKIARTKLRTIPGLKVSAEDISVVGGGIRNVPIQYSIR
                     GQDLYALQKYAKKITAEFSKLPGIVDVDTSLEVGKPEFKVYIDRDRAAALGVDVASVA
                     EAINLLISGELDIARYKDELKGKRYDIRVRLNPEDRASAEGMQRIAVRARDGKLVELA
                     NVVKVEEGTGPSVINRADRQRAITVFASLEGKPLGQAKEELDAIAGRILPVDYMPKYY
                     GMADVMTESFGYLLFALLLGVIMAYMILAAQFESFIHPITVLLSLPLSFVGAFGALFL
                     TGKTLNIFSFIGLILLMGLVKKNAILLVDYTNVLRGRGLSRRDAILQAGPVRMRPILM
                     TTFAMVFGMLPIALGVGEGAETRAPMGISVIGGLLTSLVLTLIVVPSAYDIFDDWQEK
                     FKNRTRKTAEK"
     gene            8800..9090
                     /locus_tag="PLQ77_04620"
     CDS             8800..9090
                     /locus_tag="PLQ77_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09993.1"
                     /translation="MKMFFMIYTEAMDERVTEAFKNAGFRSYTKMHGATGEGEESEPK
                     LGTHYWPGRNNTLLLGVPDEEVQKLVDLVRKFKQENPRAGLRAFTFPLEECV"
     gene            complement(9103..11226)
                     /locus_tag="PLQ77_04625"
     CDS             complement(9103..11226)
                     /locus_tag="PLQ77_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPL09994.1"
                     /translation="MTDAIKQISLDVLVAMNTVVKNVRFYPLSSALVMNGMENLHQVF
                     LNYFKAYPSFILSESEKTLLVGDEPLTQKDHEKSYVASTLEILLKFNIKSMSLHRGLE
                     KRELLTLLSYLAKDPEKILDEGGLPELIEKNHLSHIRLDEKIFVAKGKNQQIVAGLEL
                     TDDQIMRYLGESHPELKGDRQKLLELTDSPEWLLRSFEESLSNLAAEKDVLPAVQFAE
                     KLTGILKRMDEVSDQMDPDTQKKIRENIDRAAAGIGEAVASEILLQHGEESASGALMR
                     HLVGRLLPDAGAAASEISKDADASGGNEAPEADSLPQIRENIERLLGDSAKGVLDQAV
                     MSSLTRTVKTLAAHKEQATVDTIMNRFLANMFAEDARVRDQAARALIEIIENLPDKEK
                     QAVLGGIAGKLLDWIRLETLATPAYRKICNFLRDHVNDLIWQDDFHAAIPILDFFNDV
                     RTGVTEKNDTIHEISVDFIDACASAERIAILFNFFKEGEPDKKRDAGEMLIRLGDAAL
                     NQLLDILKDHRDSNERVRIMRLIISAGRRAIPPVRDRLGKDQPWFYLRNIAYILSHIG
                     DESSAEAMQPLLLHENIKVRMEALKSISRTGKSQRCTLLLSVLPQVEHKFMINLVDAL
                     GNAKCAEAEPTLLILLENNPIRDKDLRATLQERICAALGSIGLPNSIPALSKIVGTKS
                     FLGISRYPENVKAAAARALASIQRR"
     gene            complement(11393..11542)
                     /locus_tag="PLQ77_04630"
     CDS             complement(11393..11542)
                     /locus_tag="PLQ77_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPL09995.1"
                     /translation="MCSICGYVYDPAEGDPDNGIAAGTRFEDVPDSWTCPVCGAEKKM
                     FEVDR"
     gene            complement(11657..12088)
                     /locus_tag="PLQ77_04635"
                     /pseudo
     CDS             complement(11657..12088)
                     /locus_tag="PLQ77_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            12253..12657
                     /locus_tag="PLQ77_04640"
     CDS             12253..12657
                     /locus_tag="PLQ77_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL09996.1"
                     /translation="MSILKKTALYFARKEYCRSAIEEKADLSAIREKPTPKILVGLVL
                     IAFSYVIGLPAVVALGVIAVWIRKPLVVIIGGPLIYAVSTIVFIVGIRMAGDKYFRVF
                     SRWLVRVVLEKILGKELQTPSGNGPDNPCPGD"
     gene            12719..12991
                     /locus_tag="PLQ77_04645"
     CDS             12719..12991
                     /locus_tag="PLQ77_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPL09997.1"
                     /translation="MSTVEEVISELKGKIIATLGLLDVTPGDIKDDDPLVGGETGIDS
                     IDVLELVMMIEKDYGVKIDSKELGARVFASVRTLATFILQAKASKA"
     gene            13050..14402
                     /locus_tag="PLQ77_04650"
     CDS             13050..14402
                     /locus_tag="PLQ77_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HPL09998.1"
                     /translation="MKKVLLISANRMKKPYPVYPLGLDYVAGALHSRYQTKILDMNHC
                     PSLGSLGEQLRAYEPDYIGFSIRNIDNTDLMNARGFLSDYRDMIGVIRRHSKAPLILG
                     GSGYTVFPLEFFHALGADYGIAGEGERLPVLLDALEQGRDASSVPGVITPDTPSIRYL
                     PWKGELHRVFDPDAEYAKFYVSYGGMLNLQTKRGCPFRCSYCTYPHIEGGKMRLLDPP
                     EVADQARRLQEAGARYIFVTDSAFNASVEHSLQVARAFRDRGITIPWGGFFAPTALPD
                     GYFRELAQAGLTHVEFGTESLSDTMLSNLRKPFAAADVFDAHRQAQTAGLYIAHYFLL
                     GGPGENRETLRETLEGADRLSRAVFFFFCGIRIYPHTALYDTAVREGQIQASQNLIEP
                     VFYRSPFLSGPEIIRTVEAHAAGRINWLIGAGEGKATRILPRLYERGHTGPLWEYLIA
                     "
     gene            14537..15361
                     /locus_tag="PLQ77_04655"
     CDS             14537..15361
                     /locus_tag="PLQ77_04655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPL09999.1"
                     /translation="MEEGKKRLSPALIAGGAALMAAFAVFFVNPLLSASVLSVYVGLC
                     VASCFLPQAHFLGPVISRGRTGQRVVALTFDDGPSEATTPKVLDLLDRHGAKAAFFVS
                     GANAARFPHLVGEMVRRGHSIGNHSMNHDPFLMLRGSRTLRREIVQAGEVLQGMGIDA
                     LAFRPPVGIVNPKLFPVLEALGLFCVTFNCRARDAGNWRIRNLSDRILKKVKSDDIIL
                     LHDRPPRGFEDPAVFWDELEKTLAGIKDKRLRIVPLSYLAGRDIMRLKSPGDPAFF"
     assembly_gap    15391..15490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15491..15737
                     /locus_tag="PLQ77_04660"
     CDS             <15491..15737
                     /locus_tag="PLQ77_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10000.1"
                     /translation="LPGIALLSAVYEAVLKDASRRGEPLRISGLKRVRFTGPVRPGEK
                     LSLVLAREIQNNEILFHFKVAVRENTVCSGLMSVAKV"
     gene            15755..16027
                     /locus_tag="PLQ77_04665"
     CDS             15755..16027
                     /locus_tag="PLQ77_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPL10001.1"
                     /translation="MPVNIELKAIHRRVAEIIIDELKLEDVTPDTFNPEMDLVDELGI
                     DSMDLATIALVLQDEYKIVIDEDDYPRLKTVRLICEYIEDKIATRH"
     gene            16048..>16921
                     /locus_tag="PLQ77_04670"
     CDS             16048..>16921
                     /locus_tag="PLQ77_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10002.1"
                     /translation="MTANPPTSVRPDRPKFRDLLARPDIRARWEKVRKYFFLRESTYD
                     MTNRCNIRCDGCYYYEGDKPFAAENLSVPDWRKLMRAEKARGITYVVLAGAEPSLVPQ
                     LLEVCYAEMPLGCIATNGFKKIPEPVGYKIHISVWGNDETSEKTRGAKHLLQKQIENY
                     RNDPRAVFVYTFTRENIEEAQGVAAQLVAAGCKMTFNVFSSPVGYAGSLRHDADTLLK
                     TREMMLALLNRYPRQVLFSAYNAVAHTSDRGLHDLYGCSYPRRNPSQDIGLGRSFRQY
                     RTDLNWDRSAACCVP"
BASE COUNT         3572 a         4513 c         5033 g         3703 t
ORIGIN      
        1 cgaacgtttt gccgctggaa tcgcggatgc tcgttgcggt gatccggaac cattttccct
       61 tttcccccag atccgggaaa aactccgtca cttcataggc atacttcagg attttggact
      121 tggcgacttt tccggcgtaa agcgagtagg agtgatcgat ctttttgctg atgatcaggt
      181 ccgccatgca ggaccgcggc gaagaataaa acgcgcgcca ttgctggtcg gtgcccagga
      241 cttcttccgc gcggatgccc gagagcccct ccagcgcacg gttccagtag aggattttac
      301 ggttggtgga gatgacaaag gtagggatcg gcgaactctg cagaatgctg cggaggatct
      361 gctggtcgtt gcgtcccctc cagatcgcgt aggcgaagct gatgacggcg gttgccgcca
      421 gaagcaggac aaaaagaaga aaatggatct ccattccagg cacccggttt tcccaatctt
      481 tccgtgaaga tcgatccggc cgccctgcgg ccgggaaaaa tgcctcacgg tcaccctcgg
      541 aatttggtgg aatctgaata gcaaggttgc cttgaaattg caagattgga aaagccggca
      601 cggaaaaaaa agttgcaaag cggcaaaaga ttgtggaaaa ggggaaacta ctttttgaaa
      661 ggcgcggcat gcagggcggt gcgggaagaa aaattctggt ggccatgagc ggcggagtgg
      721 attcgtcggt cgctgcgctg ctgcttcggg aggcgggcca tgagattgcc ggggcgacga
      781 tgctgctggg cgttcaccgg ccgggggacg cccccgggcg ttttacggat gagtccgccg
      841 ccgacgccca aagagtctgc gatcagcttg ggattcccca ccggaccttt gattttgccg
      901 acttgatgga aaaccgggtg attgccagat tcatccgcga ataccaaagc ggacggacgc
      961 ccaatccctg cgtggattgc aaccgccatc tgaaattcgg cgcgctcttc gcgaaagccc
     1021 gcagtctggg ttttgattgc ctggcgaccg gccattacgc aagaatcgta aagcggcaaa
     1081 gccgctgggc gctgatgcgg ccaaaggatg agtccaagga ccagacctac tttttgtatc
     1141 cgattcctgc cggggatctg ccgtcgattc tttttccgct gggcgccctg accaaaacgg
     1201 aggttcgtgg gatggcaagg cgggccgcct tgcaaacggc cctccgcgag gacagccagg
     1261 acctttgctt cgtgacggcg gggaattacc ggcaattgtt tgaagaccgg cgcctgccgg
     1321 tggttcccgg cgagattgtg aatccggcgg ggcaggttct cgggcgccac cgggggattg
     1381 tctcctacac catcggacag cgcagcggcc tgggcatcag cgcgaagacg ccgctttacg
     1441 ttctgggatt tgacccggcg aaaaaccggg tggtggtcgg gaagcgggag gaactccggt
     1501 cttcgggctg catcgccggt gacctgaata tgcttgccga ccggtttccc gaagacgtgg
     1561 aggcgaaaat ccgctaccgg aaaaggccgg tgcgctgccg cgtcatcagg gaaggcgaca
     1621 ggatgaaggt tgtcttcgag caggcccagg aagcggtgac tccgggccag tccgtcgttt
     1681 tttacgcggc cgatgaagtg ctgggcggcg gcgtcatcga agaggttatc cggtaatgac
     1741 cgggggtttg ccgaaggatt attttaacgt ttattttttg cggaaaatat actaaacagt
     1801 gcgcaccaat gtcaaaatgg agccaaacat gaagacaaca tgccggggcc tgcgggtctt
     1861 tatttttgcc ctctcgctga tgctttgccc cgctgccgta tccgcccagg cgctgaccct
     1921 ggaggatagc atcgccgtgg cgctgcaaaa cagccatgtg ctgaacatcg ccaaagaagg
     1981 ggtcaagggg gccgaggcgc agaagaagga ggccctgacc ggttttctgc cgaagttcag
     2041 caccacttac agctacacgc gcctcaatga agatcccaac ttctactttc ccggatttgc
     2101 cgggccgccc gttatccagg gagggcccat gactaccggg accatcaaca actacaactg
     2161 gggcatcgag gcccgtcagc cgctttttgc cggcggcgga atcctggcca actaccaggc
     2221 ctttaaaatc gccgaggata cggcgctggt ggagcagacg gccaaatccc tggacgtcgt
     2281 ccaggatgtc aagatcgcct atttcaatat tttgcgcacg caaaggctga tggagacggc
     2341 ccggcagcag gtggacatgc tggccgccca ctgcgatgtg gccgagaatt tttaccgggt
     2401 cggcctgatt cccaaaaacg acatgctgca cgcggaagtg gagctggcca acggaaggca
     2461 gacgctggtg aaggcgaaaa acggcgtcga atcgaccaag gcgcgattga acacgatctt
     2521 gaagagggaa gtttggacgc ctctcgaggt ggtcgatatt ctcgactatc agcccctgcg
     2581 tcaatcgttt gaagagtgcc tggccgttgc ccggcagcgc cgtccggagc ttaaaatgtc
     2641 ggagctccgg gtcgaacagg cgggcaagat ggtccgcgcg gcgcaaagcg agtttttccc
     2701 ggcggtcaat ctggtcggaa attacggacg gttcggcgat accgcatcgc tttccggcac
     2761 gcagtacaag gacacggaaa gctggcaggt catggcggtg gccagctgga atttctggga
     2821 gtggggcaaa acgaaatacc gtgtggacgc cgggaaagcc agggaaaacc aggcactgga
     2881 tcagtcccgg gagctcaacg accagatcgc cctggagatc aagaacgcct ttctggcgct
     2941 gcaggaagcc gaaagccaga ttgccgtttc aaaaaaagtg atcgaacagg ccgaagagaa
     3001 tttccgcatc gccgaagccc gctacaagga gcgggtggcg cgctccacgg aggtgcttga
     3061 cgcgcagacg ctcttgaccc gcgccaaggc cgagtatgcc aatgccctgg ccgattacaa
     3121 tattgctcac gcccgcctgc aaagagccat gggagttgtc cgttgaccgc aaaacgtttt
     3181 tgggacgaaa aaggatattc acgagttaag caattgccgt tgtacatgat gattttgtgg
     3241 accgcgctcc tgctgtgttc cccgacggcg ttcgcgcagg aatacagcct gcgggacctg
     3301 tatgccgagg ccgtgaaaaa ttccgaaaaa atcaagtatg ccgaggaaaa cctctacatc
     3361 gcgaagatgg gtaaggagaa ggcttgggcg gttttaatgc ccagagttac tgcgtttggc
     3421 acgtacaatc gcttttcgga aaaaaaatac ggggatcccc ctgcctatgg caatgttctc
     3481 attcagccaa acgaatcagg caactggggc gtccgcgccg acgagagttt ttcgctgagc
     3541 atccgcgaac tggacgcctt gaaaatctcc ggacagctta tttccaaaag cgagtttgat
     3601 ctggaaagca ccaagtcgga ttttattttt ttcgtggcga cggcctatta cgacgccctc
     3661 aaagcgaaaa aagcgctgga aatcgccgcc gccaacgtgg agcgtctgac gcagtaccgc
     3721 tattttgtcg agaaacggct gaaggtgggc gaggtgaccc gtacggctct gctgcgcgcc
     3781 gacggcgaat tgtccggggc gcagtccgat tatcttcggg ccaccaacgc cttccagctc
     3841 gcccgcgcgg ccctggtccg cgtcacggga atcgaggaaa acttccggct cagagaggag
     3901 gcgcccgact tctccgccgt cgatgcgttc gatcgtctgc ggcagaccgc ctttcaaatg
     3961 aggccggacc tgaaaagcta cgacgtgcag attcaggtct ccgagcggca ggtcaaatat
     4021 gcccggggcg ctttctggcc gtatgtcggt ttttttgcca tttacaacgg cgctgatcaa
     4081 aatcctgcaa cttcaacgct caaccgggaa agtgttctgg ccggcgtttc cctgaatttt
     4141 ccgttttttg aagggggctt gagagtggcc gagctcaagg aggcaaggtc cagggagcgg
     4201 caggcgaggc tggcgtatga tgatctgaag aaaaacgtcg acatcgagat tcgcggagcc
     4261 tggctggatc tggaaaccca gagaggcacg ctgaaatttc tggaagatca actggttttt
     4321 gcccacgaca actacaatgc cgtcctgagg cagtaccaaa acggtctggc gaccagcctc
     4381 gacgtgatgg atgccaacac gctgctgctc acgtcggagc gcaacacggt ggatgccttg
     4441 tacggttgtc agctggcgca tttgagggtc agaaaatcaa gcggcacgct gcttcggttt
     4501 gttcaggaag gctattgacg aaagcggcgg cgtcgggtcg ggaaggggag cgaaccatgc
     4561 gttttttaca atatcaaaat tccggcggac aggcggcgcg acgtttgtcc gcactgatcc
     4621 tgctcacgct cgcggttttg acgctcgcgt cctgccagag ggaaaaggcg gccgaaccgg
     4681 aaaggctggt gaatgtgcgc atttggcctg ccgaaacgaa aagggtagag ccctatctgg
     4741 aaacgaccgg gacactcaaa gcttttgagg aagtcatcgt cagttccgaa gtggacggta
     4801 tcctcaggcg gatcgaagtg gatgaggggt cgcccgtcca cgcgggcatg ctgcttgcgg
     4861 aaatcaatga taccgactac cggctggacc ggaaacggtc cgatgccgcg ctcaagcagg
     4921 ggcgggcctc gctggacaac gcccgtgcgg agtataaaag aaaagaagcc ctctaccggg
     4981 aggagctcat caccaaacag cagtttgacg atgtctcgac gcgggtggcg ctggcggaag
     5041 cagagctgga aagaatccgg gcaacactgt cgatctcgca ggaaaggctg gcgcggacaa
     5101 aaatccattc gccgctttcc ggcgccgtca aggaaaagaa agtgtccgcg ggcgattacg
     5161 tgcgcaacgg cacgccgctt tttcagctga tcaggataca tccattgaag ctcaatttta
     5221 ccgtcagcga aaaagacgtt gccgccttga aaatcggaca ggaagtgagc ttcaccgtcg
     5281 acgctttttc cggcaaaacc tttcgcggcc gggtgaatct gctgtatccg aacgtggagg
     5341 aaagaacccg cacgctgcag gcggaggccg ttgttcccaa tgccgatcat ctgttgaagc
     5401 cgggatattt cgcccgcgtt caaatctaca cgcaggcggc gcgggatgcc gtcttgattc
     5461 ccgtaacggc cctcctgtat gacggccccg tgatccgcgt ttttgtggtg cgcggcaatc
     5521 aagcgcagga aagaatcatc aagacgggca acaaatacgg cgaaggggtg gaaattctgg
     5581 aaggcctcaa ggaaaaagag caggtggtcg tcgtcggcca gaacaatctt tcggatgggg
     5641 tgaaggttca tgtggctcgc tgatacctcc gtcaaacgcc cggtcttcgc caccatggtg
     5701 atcatggccc tggtggtcct gggcgtcgtc agttatccgt ctatcggcgt cgatcttttt
     5761 ccgaaaatcg attttcccat tgtcaccatt acgacgaccc tgaaaggcgc cagccccgac
     5821 gtgatggatg tggacgtcac ggaccgcatt gaaggcgcgg tcaacaccat caacggcgtc
     5881 aagaccatta cgtccacgag ctatgaaagc atgtcgcgca cgaccatcga attcgttctg
     5941 gagagggata tcgatcaggc cgttcaggac gtgcgcgaaa aagtgtccgg gattcgcaac
     6001 aaactgcctc aggatattga ggaaccgagg attgccaagg tggaccccga cgctcagccg
     6061 attttgtttc tgaacctttc cggaaaccgc tcggttcgcg atttgtccat gtatgccgat
     6121 gaagtgctca aggaacagct tcagcggatc agcggcgtgg gcgacgtgat tttctatgga
     6181 ctgcgcttac ggcaggtgcg catctggctt gatgccgcca aaatgcaggc ctatcaggtt
     6241 gctcccagcg acgtggtcct ggccctgaag cgtgaaaata tcgagctgcc gggggggcgg
     6301 attgaaaccc ggaccaagga atataccgtc cggattaaag gggagtttgc caaaatcccc
     6361 gatttcaacg acctgatcat cagttactac aaaggcgcgc ctgtccgtgt ccgggatatc
     6421 ggccgggcgg aagacggcat ggaagaaaaa cggtccctgt cgcgttttaa ccggattccg
     6481 gccgtcggca tggccattca gaaacagtcg gggaccaaca cggtggaggt ggccgaccgc
     6541 gtgaaagcgg aagtggcaaa aatcaacaaa acgctgcctc cggggatgaa ggtcaatacg
     6601 gcggtggatc agtcggtttt cattgttcgt tcgatcgacg aggtgcagtt ccatttaatc
     6661 atcggcagta tttttgccat tctggctgtt tttatttttc tgaagaacat ccgcacgact
     6721 ttaatcagcg ccgtcgcttt gcccgtttcc gtgatctcga cctttgcgct gatcaacgcc
     6781 ttcggcttca ccttcaacaa catgaccatg ctggcgctgt cgctctcggt ggggttgctc
     6841 attgacgacg ccatcattgt cattgaaaat atttaccgtc acgtggaaga gggcatggaa
     6901 ccgcgggaag cggcgacttt tgcaacctcg gagatcggtc tggccgtgat ggcgacaaca
     6961 tttgccattg tcggcatctt tctgccggtg gccttcatga aagggatcat cggccggttt
     7021 ttcctccagt tcgcgctgac gattattttt gcgatcatgg tttcgctgct ggtttccttt
     7081 accctgaccc ccatgatggc atcgatcttt ctcaaaccgc ataaaaaatc catccccaat
     7141 cccctggccg cgggtggggg aggcggcgga aatgttcaga aacccatctg gacccgaatc
     7201 ggcgatcgca tggagtattt ttatgcaaaa ctggaatcgt cttaccggac ggtcctggaa
     7261 tggacgctgg gctggcgcaa aaccgtgctg gtctgcgccc tgatgatctt tcttttgagc
     7321 ctggggctta cggcttttat tgggaaggaa ttcaacccgc ctgaagatca gggcgtcttc
     7381 ctgattcgca tggaagcgcc gatcgattat tccgttttgc agatagagca gtatctggga
     7441 aaaacggaag ccatgatgca ggaaattccg gaaatcaaat ctgtttttta tgttcaggga
     7501 tatggcgggt accccaatcg cgcaatgatg atgctcaatc tcacgccaaa ggctgaacgg
     7561 aagagaagtc aggaagatat caagaaaatc gcgcgaacga aattgcggac gattcccggg
     7621 ctgaaagtat ccgcggagga catttcggtg gtcggaggcg gaatcagaaa tgtgccgatt
     7681 caatacagta ttcgcggaca ggatctctat gccctgcaaa agtatgccaa gaagatcacg
     7741 gcggaatttt ccaaactgcc ggggatcgtg gatgtggata cgtcgctgga ggtcggcaaa
     7801 ccggaattca aggtttacat cgaccgtgac cgtgcggccg ctctgggcgt cgatgtggca
     7861 agtgttgccg aggcgatcaa tttgctgatc agcggagagc tggatatcgc ccgctataaa
     7921 gatgaactca agggaaagcg atacgatatc cgcgtgagac tcaatccgga ggatcgggcc
     7981 agtgcggagg gaatgcagcg cattgccgtg cgcgcccgcg acgggaaact cgttgagctg
     8041 gccaatgtgg ttaaagtaga ggaaggcacc ggtcccagcg tcatcaaccg cgctgaccgt
     8101 caaagggcca tcaccgtgtt tgcctcgctg gaaggaaaac ccctgggaca ggcaaaagaa
     8161 gagctggatg cgatagccgg acgcatcctg ccggtggatt acatgccgaa atattatgga
     8221 atggctgacg tcatgacgga atctttcggt tacctgcttt tcgccctgct tttgggtgtg
     8281 atcatggcgt atatgattct ggccgcgcag ttcgaaagct ttatccaccc cattacagtt
     8341 ttgctttccc tgcccctgtc ttttgtcggc gctttcggcg ccctgtttct gaccggcaaa
     8401 acgctcaata tcttcagctt tatcggcctg attctgctga tgggtctggt caagaaaaac
     8461 gccattttac tcgtggatta cacgaatgtt ttgcgcggcc ggggcctgtc gcggcgcgac
     8521 gccatccttc aggcgggacc tgtccggatg cgccccattc tcatgacgac gttcgccatg
     8581 gttttcggca tgctgccgat cgcgctgggc gtcggagagg gggcggaaac tcgcgctcca
     8641 atggggatat cggtgattgg cggactgctg acgtctctgg tgctgacgct gattgtggtt
     8701 ccgtccgcct atgatatttt tgacgactgg caggaaaaat tcaaaaaccg gacaagaaaa
     8761 accgcggaaa aatagacatc ggctgtttcg ggagggcgca tgaaaatgtt ttttatgatt
     8821 tacaccgagg cgatggatga gcgggtcacg gaagccttta agaacgcggg ttttcgatcc
     8881 tacaccaaaa tgcacggcgc gaccggcgaa ggcgaggaaa gcgagccgaa gctgggcacg
     8941 cactactggc ctggaaggaa caataccctt ttgctgggcg tgccggacga ggaggttcaa
     9001 aagctcgtcg atctggtccg gaagttcaaa caggaaaatc cgcgcgcggg cctcagggcg
     9061 ttcacctttc cgctggagga gtgtgtctga ggcgggagct ccttaccgcc tttggatgga
     9121 cgccagcgcc cgggcggccg cagctttcac attttccggg tagcggctga ttcccagaaa
     9181 ggatttggtc ccgacgattt tggaaagggc ggggatggaa ttgggcagtc cgatgctgcc
     9241 cagagcggcg cagattcttt cctgcagggt cgcccgcaga tctttgtccc ggatgggatt
     9301 gttttccagc aggatcagca gggtcggctc cgcctcggcg cactttgcgt tgccgagagc
     9361 gtcaacgagg ttgatcatga atttgtgctc cacctgcggc aggacggaaa gaagcagcgt
     9421 gcagcgctgg cttttcccgg tgcggctgat gcttttgagc gcctccatcc gcaccttgat
     9481 gttttcgtgc agaagcagcg gctgcatcgc ttcggcgctg gattcatctc cgatgtggct
     9541 caggatatag gcgatgttcc gcaggtaaaa ccagggctga tccttgccga ggcggtcgcg
     9601 gaccggcgga atcgccctcc ggccggcgct gatgatcagc cgcatgatgc ggacgcgctc
     9661 attgctgtcc cggtggtcct tgagaatgtc cagaagctgg ttcagcgctg cgtctccgag
     9721 ccggatcagc atctcccccg cgtcgcgctt tttatccggc tccccttcct tgaaaaaatt
     9781 gaagaggatg gcgatgcgct cggcggaggc gcaggcgtcg atgaagtcaa cggatatttc
     9841 gtggatcgta tcgttttttt ctgtcacgcc cgtccggaca tcgttgaaaa agtccagaat
     9901 cggaatcgcc gcgtgaaagt cgtcctgcca gatcagatca tttacgtggt ccctgagaaa
     9961 attgcagatt ttccgatagg cgggcgttgc cagcgtttcc agtctgatcc aatccagcag
    10021 tttgccggcg attcccccga ggacggcctg cttttccttg tccggcaggt tttcaatgat
    10081 ttcgatcagg gcacgcgccg cctggtcccg cactctggca tcttcggcaa acatattggc
    10141 cagaaagcgg ttcatgatgg tgtccaccgt tgcctgctcc ttgtgagccg ccagggtttt
    10201 caccgtccgg gtgagcgacg acatgaccgc ctgatccaga acgccttttg cgctgtcgcc
    10261 cagcagccgt tcgatgtttt cccgaatctg cggcaggctg tccgcctccg gagcttcatt
    10321 gccgccggat gcatccgcat ccttggagat ttcggatgcc gccgcgcctg cgtccggaag
    10381 gagtctgccg accagatgcc gcatcagcgc gccgcttgcg gattcttccc cgtgttgaag
    10441 cagaatttcc gaggcgacag cctctccgat gcccgctgcg gcccggtcga tattctccct
    10501 gatttttttc tgtgtatcgg ggtccatctg atccgatact tcatccatcc gcttcaggat
    10561 tccggtcagc ttttccgcga actgaaccgc gggcagaacg tctttctcgg cggccaggtt
    10621 ggaaaggctt tcctcaaagg accgaagcag ccactccggg ctgtccgtca gctccagaag
    10681 cttctgccgg tcgcctttca attccgggtg ggattcgccg aggtaccgca tgatctggtc
    10741 gtcggtcagt tccaggccgg cgacaatctg ttgatttttc cccttggcaa cgaatatttt
    10801 ttcgtccagg cggatatgcg atagatgatt cttctcgata agctccggca ggccgccctc
    10861 gtccaggatc ttttccgggt cttttgcaag gtaggacagc agagtgagca attcccgctt
    10921 ttccagcccc ctgtgcagcg acatgctttt gatgttgaat ttcagcagaa tttcaagggt
    10981 cgaggcaaca taggattttt catggtcttt ctgagtcagg ggttcatcgc cgacaagcag
    11041 ggttttttcc gattccgaca ggataaacga tggatatgcc ttgaaatagt tgagaaaaac
    11101 ctggtgcagg ttttccattc cattcatgac gagggcgctg ctgagcggat agaagcgcac
    11161 gttcttgacg accgtattca tggcgacaag gacatccagg ctgatttgct tgattgcatc
    11221 cgtcaagatt gttttctcct gccgttcatc attttatgac cgggggcagc atccggttga
    11281 tcgtatgtca taaaatttta aaggaaaata accctataaa aaatgcggca aagtgtcaag
    11341 catcacctgc aacgaagggc cggcgcctgt tctttgtcct gatgcctgaa ccttaccggt
    11401 ccacttcaaa catttttttc tccgcgccgc ataccgggca tgtccaggaa tcggggacgt
    11461 cctcgaatcg ggtgccggcg gcgatgccgt tgtcggggtc gccctcggcg ggatcgtaca
    11521 cgtagccgca gatgctgcac acatagcggg ccgcggccgg agctttcggt ttggcggcgg
    11581 ccgcgtcggc ttcgtacgtc ggggcggatt tgggggattt gcctcccttg acgttctgat
    11641 agtaggcgta tgtcatcggc gtgccttccc ccgccaggtc cgcttcgacc acatttccaa
    11701 ggaaaatcgt gtgggtgccg ccgtccagct cacctgccac ttcggcttcg atgaatccca
    11761 gggcatgatc cagaacaatg ggcacgccgg tcgcgccggt tttgcttttc acgcctttga
    11821 gcttatccac gtcgcggccg cttttaaagc cgaaaagacc gatgaaggcc atcggggcgg
    11881 cctccgagag aatggaaacg acgaattttc cgctttcccg tatgagggcg tgcgtgtagt
    11941 tttccttgtt gatgcagatg gccagggtgg cgggcctgga ggtgacctgg acgatggagt
    12001 tggcgatctg accgttgaat ttgccgtctt tgccggacgt gacgatgtaa acgccgtagc
    12061 tgatttggtg gagggctgct ctgttcatgg tttttattcc tcttttccgt ataaatttat
    12121 cgtcaaacct tatagctgaa tttgcctttt catgccatga aattatgagg gataaaaaaa
    12181 tattgcaaat ctttgccgat tgcagatatg atgcccgagg tttgtaaata agctgtccgg
    12241 ttgagactgg ttttgagcat tctcaaaaaa acggcccttt attttgcccg caaagaatat
    12301 tgcaggtcgg caatcgagga aaaagccgat ctgtcggcca tcagggaaaa accgacgcct
    12361 aaaatcctgg tcgggctggt cctgatcgcg ttcagttatg tgatcggttt gccggcggtc
    12421 gttgccctgg gggtcatcgc ggtctggatc aggaagccgc tggtggtgat catcggcggg
    12481 ccgctgattt acgccgtttc cacgatcgtt tttattgtcg ggatccgaat ggcgggggat
    12541 aagtatttcc gggtgttttc ccgctggctt gtccgggtcg tgctggaaaa aattctgggg
    12601 aaggaactgc aaacgccgtc cgggaatggc ccggacaacc cttgcccggg agattgaagc
    12661 gcgcggggct gcggcttggc gccgtgaaaa agttttcatt aatttgtcga ggtgaagcat
    12721 gagtacggta gaagaagtta tcagtgaact gaaagggaaa atcattgcaa cgctggggct
    12781 gctggacgta acccccgggg atataaagga tgatgatccg ctggtcggcg gcgagacggg
    12841 cattgattcc atcgatgtgc tggagctggt gatgatgatt gagaaggatt acggcgtcaa
    12901 gattgacagc aaggaactgg gcgccagggt gtttgcttcg gtccgcacgc tggcgacgtt
    12961 cattctgcag gccaaagctt ccaaagcctg attcctgccg gggccggaaa cggccctgtt
    13021 ctttcaacca ttcaaagcag aatgttttca tgaagaaagt attgctgatt tccgccaaca
    13081 ggatgaaaaa gccctatccg gtttatccgc tggggcttga ctatgtggcg ggcgccctgc
    13141 attcccggta tcaaacgaaa attctggaca tgaatcactg tccgtcgctt ggaagcctgg
    13201 gggaacagct tcgggcgtac gaaccggact acatcggatt ttcgatccgc aatatcgaca
    13261 acacggacct gatgaatgcc cgcggtttcc tttccgacta ccgggacatg atcggcgtca
    13321 tccggcggca ttcgaaggcg ccgcttattc tgggggggag cggctatacg gtttttcccc
    13381 ttgaattctt ccacgcgctc ggcgcggact acggcatcgc cggagaaggg gagaggctcc
    13441 ccgtgctgct cgatgcgctg gagcagggcc gggacgcttc gtctgtcccg ggagtgatca
    13501 ctcccgatac tccgtccatc cgctatcttc cctggaaagg agagctgcac cgggtctttg
    13561 accccgatgc cgaatacgcg aaattttatg tttcgtacgg cggcatgctg aatttgcaga
    13621 cgaagagggg atgcccgttt cgatgcagct actgcaccta cccccacatc gaaggcggta
    13681 aaatgaggct cctggacccg ccggaggtcg cggaccaggc ccgccgtctg caggaggccg
    13741 gcgcccggta catctttgtg accgattccg cgttcaacgc cagcgtcgag cacagccttc
    13801 aggttgcccg ggccttcagg gatcggggga taaccattcc ctggggagga ttttttgccc
    13861 ccaccgcctt gccggacggc tattttcgcg aactggcgca agccggactc acgcatgtgg
    13921 aattcggcac ggaatcgttg tccgacacga tgctgtcgaa cctgaggaag ccttttgccg
    13981 ccgccgatgt ctttgatgcc caccggcagg cgcaaacggc cggtctgtac atcgcccact
    14041 attttttgct gggcggcccc ggcgaaaacc gggagacgct gcgggagacc ctcgaaggcg
    14101 ccgatcggct gagccgggcg gtttttttct ttttctgcgg catccgcatt tacccgcata
    14161 cggccctgta cgacacggcc gtgcgcgaag gccagattca ggcgtcgcag aatctgatcg
    14221 aaccggtttt ttaccgctcg cccttccttt ccgggcctga aatcattcgg acggttgaag
    14281 cgcatgccgc cggaaggatc aattggctga tcggggctgg tgagggcaag gcgacacgca
    14341 tcctgcccag gctctacgag cgggggcata cggggccgct gtgggaatat ctcatcgcat
    14401 gacagcggtc cggttttttc gcggggaggg tcggtttgac ctcgcgccat ctcgcgcaac
    14461 cttcaggtga tcaggccgtc ggggcattca acccgttttg cgcgggttcc aagcggcgcg
    14521 cccggagagg cttctcatgg aagaaggaaa aaaacggtta agccccgccc tgattgcggg
    14581 gggtgcggcg ttgatggccg cgtttgcggt ttttttcgtc aatcccctgc tttcggcttc
    14641 cgtgttgtcc gtgtatgtgg ggttgtgcgt agcgtcctgt tttttacctc aagcacactt
    14701 tctggggccg gtcatcagcc ggggacgcac ggggcaaagg gtcgtcgccc ttacttttga
    14761 cgacggtcct tccgaagcca cgacgccgaa ggttctggat ctgctggacc gccacggcgc
    14821 gaaggccgct tttttcgtgt ccggcgccaa cgccgcgcgc tttccgcatc tggtaggcga
    14881 gatggtccgg cgcgggcaca gcatcggcaa tcattccatg aaccatgatc cttttttgat
    14941 gctgaggggg agccgcacgc tccgccgtga aattgttcag gccggagaag ttcttcaagg
    15001 gatgggcatc gacgccctgg cattccgccc gccggtcggc atcgtcaatc cgaagctttt
    15061 tcccgttctg gaggcgctgg ggctgttttg cgtcaccttc aactgccggg cgcgggatgc
    15121 gggtaattgg cgcattcgga atctttccga caggattctc aaaaaagtaa aaagcgacga
    15181 tattatcctt ctgcacgacc ggccgccccg gggtttcgaa gacccggccg ttttttggga
    15241 cgaattggaa aaaacgctgg caggcatcaa ggacaaaagg ctgcggatcg ttccgctttc
    15301 atatctggcg ggcagagaca tcatgcggct gaaatccccc ggcgatccgg cttttttctg
    15361 aaattccttt cgcttgcgcc tgccgggaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn cctgccggga atcgctctgc tcagcgccgt ctacgaggcg gttttaaagg
    15541 acgcgtcccg ccggggagag cccctcagga tttccgggtt gaagcgggta aggtttaccg
    15601 gcccggtccg gccgggggaa aaactgtccc tggtgcttgc ccgtgaaatt cagaacaacg
    15661 aaattctgtt tcacttcaaa gtggcggttc gggaaaacac ggtttgcagc ggcctgatgt
    15721 cggttgcaaa agtataggga taaaggaggc tgaaatgccc gtaaacattg aattgaaagc
    15781 cattcatcgg cgtgtggccg aaatcatcat agacgaactc aaactggaag acgtgacgcc
    15841 ggacaccttc aatccggaga tggatctggt ggacgaactg ggcattgaca gcatggatct
    15901 ggcaacgatc gcgctggttt tgcaggatga atacaaaatt gtcatcgacg aagacgatta
    15961 tccccgattg aaaaccgtac ggctgatctg tgaatatatc gaggataaaa tagccacccg
    16021 gcattgaagg caaaagaccg atattccatg accgcgaatc ccccgacatc cgttcgaccc
    16081 gaccggccga aatttagaga cctgctggcc cggccggaca tcagggcgcg atgggagaag
    16141 gtgcggaaat atttttttct gcgggaatcc acctacgaca tgaccaaccg ctgcaacatc
    16201 cggtgcgacg gatgctacta ttacgaaggg gacaagccct ttgccgcgga aaatctttcc
    16261 gtcccggact ggcgcaaact gatgcgggcg gaaaaggcgc gcggcatcac ctatgtagtg
    16321 ctggcggggg cggagccgtc cctggtcccc caactgcttg aggtctgcta tgccgaaatg
    16381 ccgctgggct gcatcgccac caacggattt aaaaaaatac cggagccggt cggctataaa
    16441 atccatattt ccgtctgggg caacgacgag acgagcgaaa aaacgcgcgg cgcaaaacat
    16501 cttctccaaa agcagatcga gaattaccga aacgaccccc gcgccgtctt cgtctatacc
    16561 ttcacgaggg aaaatatcga ggaagcgcag ggtgtggccg cccaactggt cgctgccggc
    16621 tgcaaaatga cgtttaatgt tttttcctcc ccggtcggct acgccgggtc cctgcgccac
    16681 gacgcggata cgctgttgaa aacccgggag atgatgctgg cgcttttaaa ccgctatccc
    16741 cggcaggtgc tcttttccgc gtacaacgcc gtggcccaca cgagcgatcg ggggctgcac
    16801 gatctttacg gctgttcgta tccccgccgc aacccgtcgc aggatatcgg cctgggccgc
    16861 tccttccgcc agtaccggac ggatttgaac tgggaccggt cggcggcctg ctgcgtgccg
    16921 g
//
LOCUS       DAOPAF010000031        16710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3508_length_16710_cov_81.704713, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000031 DAOPAF010000000
VERSION     DAOPAF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3508_length_16710_cov_81.704713"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1535)
                     /gene="xseA"
                     /locus_tag="PLQ77_04675"
     CDS             complement(198..1535)
                     /gene="xseA"
                     /locus_tag="PLQ77_04675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPL10003.1"
                     /translation="MKDILTVSQLNDNIKTFLEESFGVLWVEGEVSNLRRPQSGHVYF
                     TLKDDKSQIRAVYFRPYGVRAVRFDLEDGMKILCRARLSAYPPRGEYQLIVESVEPQG
                     LGALQKAFEQLKARLAAEGLFDASRKKSLPFLPRRIGVITSPTGAVIRDILNITRRRF
                     PSVNILIAPARVQGAQATGEIITALRRLHAWGEVDVIVIARGGGSLEDLAPFNDEALA
                     REICRSTIPVVSAVGHETDFTVCDFAADLRAPTPSAAAELVVPRKIELQENLHDLRLR
                     LLAAQNRCLARQRERLASLAGRFRDPRRFLADYAIHVDDLRERLKRALAHQTESAAGR
                     LKPLQARLLGFDPRRRIREQSLLLSGLAKSLRGGWNHFLWERREKLQKNAALLSSLNP
                     LAVLRRGYSITRRLPEGEIVRDAKQLAPGNRLGIQLASGRIEARVEALQGEQA"
     gene            1717..3075
                     /locus_tag="PLQ77_04680"
     CDS             1717..3075
                     /locus_tag="PLQ77_04680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPL10004.1"
                     /translation="MSENYDLIVIGGGPGGLAAAQTASAANKRVLIIERGGWGGTCTH
                     RGCIPTKAMLACSRFYADLKKLKRVGVNVSGGAVDFAAMKKHRDQMVRIAALGAQKMI
                     AGPRVESKIGLGEILSPREVRCTGEDGVRQFLKTEKILIAWGSKPQILPGLRASERIR
                     TSDEILSLEQLPSSIIIVGGSFIGVEYATLFAELGVRVTLVELMDRILPREDEEAAEL
                     LGQELARLGVVLHTSTRLVRLEESGEGVKLSARKNGEDLEIQALYALLCTGRAPVLHA
                     DQLDSLGVDFTNAGIRVDKRMMTSVGGIYAAGDVTGGMMLAHRASLQAKVAVEGMFGG
                     SRYRYDEDLIPSVVYSHPQIARVGLTERRALDEGLEISVVRSDYGANLMARAELMGQG
                     FVKIIFHREVIAGAVAAGDHAAELLAPLALAVSGKWTRRQFRSWVLPHPTLGEVFAPL
                     AD"
     gene            3176..3526
                     /locus_tag="PLQ77_04685"
     CDS             3176..3526
                     /locus_tag="PLQ77_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPL10005.1"
                     /translation="MKIIHYASAAPVHVDNDQAKGIDARVVIGKNDGADHFCMRIFEI
                     APGGYSPKHAHAWEHELFIHSGEGEAYDRGAWRPICPGTVLFIAPGEEHQIRNTGARK
                     LVFACLIPSGVPEL"
     gene            complement(3596..4465)
                     /locus_tag="PLQ77_04690"
     CDS             complement(3596..4465)
                     /locus_tag="PLQ77_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPL10006.1"
                     /translation="MKIQDVKNVCVVGAGNMGHQIALQCALSGFKTVSTDVVPAVLEK
                     AQKFVEGYLPGRVQKGKMTEEAAKKARSLISFTGDIQEAAKDADIVIEAVLERIDLKR
                     KVFADLDKYAPPSAILATNSSFIVSSRIADATKRPSQVCNLHFFNPALVMKLVEVVQG
                     PHVSDETAQCMMDFCLKIDKIPIHIKKEVDGFVLNRIFAVIQREALWMLEMGVASFED
                     IDKACVYGAGHPMGPFRLNDLTGIDLTYDICMSHFLESGNPADLPSPSVVKKVTEGKF
                     GQKTGEGWYSYKK"
     gene            4667..5293
                     /locus_tag="PLQ77_04695"
     CDS             4667..5293
                     /locus_tag="PLQ77_04695"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL10007.1"
                     /translation="MTRKTVAGKEETPRKSSALFLKRQSQIIKKATKLFMKQGYAQTS
                     MREISKATGIDIRNLYYFIRSKEEILFLVIEMIHRRELELFEKQGFTSIEDPVLQLRT
                     VVREFVDAGYGYGQEILLLYRESKSLPNRLLKVILSRESQVVARIEEILQRGRENNVF
                     RFEDASFTANMIVYELSLHALRNWNMKQYSKEELTDLLEKHVIKAVTA"
     gene            complement(5350..6462)
                     /locus_tag="PLQ77_04700"
     CDS             complement(5350..6462)
                     /locus_tag="PLQ77_04700"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPL10008.1"
                     /translation="MIQSRLCKMIGIKYPIIQAGMGPFSNNNLCVAAANAGVLGLLST
                     SGLFNKNDQPWIYKAFVESGEAGMDDDMATVMEKVLKRTYRLVKDKGGIFGINVMVSA
                     EVKPYSTIMIDTAIRVREENPDMKNNFKVIFTSAGDPVGWKEKIKGAGFTWMHVVPSV
                     KGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPAVVDAVSDENTLVAGAGGFCD
                     GRTLAAALVLGADGAQMGTRFLATKESDFHQIWKEGVVAAQDRGTLVARGFVGPARWL
                     KTPRSEEHAQNTLKKSPGVFLGTPDDYSTTDMSLIEFEIESIKAVYAGDREKALMAAG
                     EVAQRINDMPSVNDMVQGIVRDAEATLRGIEKKHLA"
     gene            6668..7528
                     /locus_tag="PLQ77_04705"
     CDS             6668..7528
                     /locus_tag="PLQ77_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL10009.1"
                     /translation="MKTGAIRVPRGTFHVRDRGNADGFPVVMLHGWPESSYCWEGVTE
                     FLDPQWRVIAPDLRGLGDSERTLDVKAYQKVELAKDVIEIINALGIQDFFLAGHDWGG
                     IVAQEMAFLIPDRIKKFVILNIPVLTNVKGAEEVTKAMIAIRYIPYWYQYFQMMPGLP
                     EAMIKGKEREWVSFFFGKKGRDGAIPRESIDEYVRCYSIENTPATAASYYRSMALDAP
                     HWAGLAGKRFSMPSLYIYGNEDSVIIPANITHLEDCFDSITVEQIKAAHFLQEEKPRE
                     VAELMNRFFK"
     gene            7582..7965
                     /locus_tag="PLQ77_04710"
     CDS             7582..7965
                     /locus_tag="PLQ77_04710"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HPL10010.1"
                     /translation="MSGIESNKDQFRALADNPGQEPFVMLNLLKFKKEGGRESYFRYI
                     RESGPFVEAVGAKVLYFGKPKELLQGSEDWDLLMLVSYPSRKAFLTMANNPDYLKVHE
                     FRAQGVERAVLYATDPVKFREILSE"
     gene            8152..9957
                     /locus_tag="PLQ77_04715"
     CDS             8152..9957
                     /locus_tag="PLQ77_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HPL10011.1"
                     /translation="MTREVSVDTVYDNPNNLVDLFEQSVVQYPNNRLFGTKNPASGQY
                     EWVTYKSVADRVDRLRGALKKLGLSKGDKVGVILHNCTEWFVCEQAVHGLGGALVPMY
                     LQELPKVIEYIVRDAGVKFLFVAHAGVYEKVKDFKNSIPTLQEIFVVFGEGENSMKAL
                     EEMGAKNPASSIKPRWSDLANIVYTSGTTGDPKGVMLSHGSLTLCARSGVDGFGLDET
                     MHVVAILPWAHVFGLAADLHDYIYCGGGIAFAESVEKLIQNFQEVKPTGLSVVPRIVN
                     KVYDTIMQGVAADPAKKQFFDAAVAEAVKNRDLPEKTKEFKDYDALVFSQVRKVFGGN
                     LKFFVTGSALIKPEIALFFKDVGQPTFDGYGLTETGPTVSMNSPKYGNKYGTVGTVDR
                     NMHVVIDKSRVGEDSLDGEIIVYGPHVMMGYHNKPQQTAEVMMPDTWNGFPGIRTGDR
                     GWLDEEGFLHITGRFKDEYKLANGKYVHPEGIENEAKLLRWILNIMLYGDGKDYNVAI
                     VVPDFAALQADSKIKPLLKDTLEDSLKEKALTDYLSGEIVAYLRKTYGGYEVPQKFLF
                     VAEDFTVGNGMLTQTMKLKRPLVMKQYGAKLQALY"
     gene            complement(10070..11305)
                     /locus_tag="PLQ77_04720"
     CDS             complement(10070..11305)
                     /locus_tag="PLQ77_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL10012.1"
                     /translation="MKSWKSLCWMFVFAFVVWGTAAQAAEIGIGFTGPLSGPAAEYGQ
                     DCYNGVDMAVKEINAAGGVTVQGKKYTFKLEKLDDRADPTLAKNNAMRFVSQNKAIAV
                     FNPVATTIGALMAIPEQNFIIAAFSSVHTIMDKKHPMIINPVPNFVAYAKNFSIIAWD
                     KGWRNGAMVVTLGAYGDAWRNVFKAVWEKKGGKILADKPANYYTETDFSAQLTAAIAT
                     KPDFLVVGGPSAPSALVIEQARSLGFKGGFVLIDQAKPDYIAKTLGNKLNLLEGMIST
                     GDIKRLPLPIMPSFMKRYNAAYKGSVVNAENCLNYNMMHIIARAVNAAQSLDAKVVRQ
                     NIVKALPTAGDKFPNELFNIEENGVMNCGTIMQYVNKGQFSKVDYVISFPKSKEEFEK
                     IKKMSKSTDPKQIRYVPLQ"
     gene            complement(11640..11831)
                     /locus_tag="PLQ77_04725"
     CDS             complement(11640..11831)
                     /locus_tag="PLQ77_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10013.1"
                     /translation="MAETPITPPENTWKCSNCGNTVVQTAPPQTCPVCQQKCEFLNVT
                     CYQPECGFTGMDNRLKPAK"
     gene            11965..12399
                     /gene="queF"
                     /locus_tag="PLQ77_04730"
     CDS             11965..12399
                     /gene="queF"
                     /locus_tag="PLQ77_04730"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPL10014.1"
                     /translation="MEKFSKKSDLAGLSLLGSGEKAKPGRKLETFPSHSKRPYTVTLS
                     TQEFTCLCPMTGQPDFAGITIQYIPDRKIVESKSLKLYFWSFRNEGVFHEHLTNIILD
                     DLVAALSPRWCKVTAQFAVRGGIAISVDAEYPEAGKSRNPKR"
     gene            12528..15728
                     /gene="carB"
                     /locus_tag="PLQ77_04735"
     CDS             12528..15728
                     /gene="carB"
                     /locus_tag="PLQ77_04735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPL10015.1"
                     /translation="MAKREDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRKLGYKI
                     ILVNSNPATIMTDPGMADVTYIEPLNAQTLTEIIENEKPDAILPNLGGQTGLNLTSEL
                     HRRGILDKHGVKVIGVQVDAIERGEDRIAFKEAMNKLGIEMPKSKPATSVEEAEKIAA
                     EMGYPVVIRPAYTMGGTGGGIVYNLEELRLIASRGISASLIGQILIEESVIGWEELEL
                     EVVRDAKNHMITVCFIENVDAMGVHTGDSYCTAPMLTISEELQKRLQKYSYDIVEAIQ
                     VIGGTNVQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISSILASGITLDEI
                     PYWREGTLDKYSPSGDYVVVKFARWDFEKFPGAVDKLGTQMRAVGEVMSIGKNYKEAL
                     QKAIRSLEKKRYGLGFVKDFHARPLDELMELLSEPSSERQFIMYEALRKGASIDALHA
                     KTHIKKWFIEQMKELVALEEKILACKGKKLPDALLVEAKKDGFSDRYLAQILEKKEEE
                     IRQQRLAAGLAESWDAVPVSGVENAAYYYSTYNAPDKVGVSKNRKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGIESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEK
                     EKPDGVIVQFGGQTPLNIAADLAKAGVKIIGTSPETIDLAEDRDRFRAMMDKLGIPMP
                     KSGMASNLQEALSVANGIGYPLMVRPSYVLGGRGMEVVHDEQMLKRYVEAAVDVTPER
                     PILIDKFLENAIEAEADAISDGTDAFVPAVMEHIELAGIHSGDSACVIPPISIPARHI
                     DTINDYTRKIAVEFGVVGLMNIQYAIANDVVYILEANPRASRTVPLVSKVCNISMARL
                     ATQIMLGKKLKDLNLKRHAFRHFGVKEAVFPFNMYQEVDPLLGPEMRSTGEVLGLADS
                     FGLAYYKSQEATGQSLPAEGTVLITVEEKDKGGILEVARAFAKIGFRIKASEGTYKFF
                     AANGIAAERILKMHEGRPNIVDAIKNGEIQLVINTPAGRLSKYDDSYIRKAAIKYKIP
                     YITTVAAAVAAVKGIEACRQGHGRAKSLQSYHAEIK"
     gene            complement(15897..16148)
                     /locus_tag="PLQ77_04740"
     CDS             complement(15897..16148)
                     /locus_tag="PLQ77_04740"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPL10016.1"
                     /translation="METDKKDSGDGSVPREEMVEVFSTYNPGDIAFVKSLLDGEGIHY
                     YFQGENTAMLVAAGAYARLLVRADQADRIRDILREAGLL"
     gene            16179..16478
                     /gene="yhbY"
                     /locus_tag="PLQ77_04745"
     CDS             16179..16478
                     /gene="yhbY"
                     /locus_tag="PLQ77_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HPL10017.1"
                     /translation="MEPLKGSQRKVLRAQAHHLKPLVMVGAKGVTDGLIGSVDLALAD
                     HELIKVKFVEFKEAKKEIAAEIAQATKSELVGLIGNIAILYRPNPDPAKRKIQVS"
     gene            16629..16702
                     /locus_tag="PLQ77_04750"
     tRNA            16629..16702
                     /locus_tag="PLQ77_04750"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16661..16663,aa:Gln,seq:ctg)
BASE COUNT         3667 a         4671 c         4825 g         3547 t
ORIGIN      
        1 gctttgccga gcagaagcga atcaggcgga tgccttcttc aaaggacttc agcgcggcat
       61 ccagcggcag ttcgcccgct tccatctccc tcacgatttc ttccagtttt tccagcgcgt
      121 cttcaaactt ttcttttgcc atcgtgtttc actcccgttg tatttttttt aagaaaccct
      181 ccgatgtccg ctgcgagcta tgcttgctcc ccctgaaggg cttccactct tgcctcgatg
      241 cggccggagg ccagttgaat gccaagccgg tttcccggcg ccaactgctt tgcatcgcgg
      301 acgatttctc cctccggcag gcgccgggtg atgctgtagc cgcgccgcag aacggccagc
      361 gggttcaggg aggaaagaag cgcggcgttt ttctgcagct tttcgcggcg ctcccaaaga
      421 aaatggttcc agccgccgcg caggcttttg gcaagtccgg aaagaagcag gctttgttca
      481 cgaatccgcc ggcgggggtc gaagcccagc aggcgcgctt gcagcggctt cagccggccg
      541 gctgcgctct ccgtttgatg cgccagggcg cgcttcagcc gttcgcgcag atcgtctacg
      601 tgaatggcgt aatcggccag gaaccggcgc gggtccctga accggccggc aagcgatgca
      661 aggcgctctc tttgccgggc cagacaccgg ttctgcgcag ccaggagacg caggcgcagg
      721 tcgtgcaggt tttcctgcag ttcgattttt cgggggacga ccagctccgc ggccgccgac
      781 ggagtgggcg cccgcaggtc cgccgcgaag tcgcagacgg taaaatccgt ttcatggccg
      841 acggccgaga cgaccggaat cgttgagcgg caaatttcac gcgccagcgc ctcgtcgttg
      901 aaaggggcga gatcctccag agaaccgccg ccccgcgcga tgacgatcac gtccacttct
      961 ccccacgcgt gaagacggcg cagggccgtg atgatttcgc ccgtcgcctg agcgccctgc
     1021 acgcgggcgg gcgcgatcag aatgttcacg gaggggaagc ggcggcgcgt gatgtttaat
     1081 atgtcgcgga tgaccgcgcc ggtgggcgac gtgatcacgc caatgcgccg cggaagaaag
     1141 ggcagagact ttttgcgcga cgcatcaaac agtccttcgg cggccagccg cgccttgagc
     1201 tgctcaaacg ctttttgcag ggcgcccagg ccctgaggct ccacggactc cacgatgagc
     1261 tggtattcgc cgcggggcgg gtaggcggaa agacgcgccc ggcacaaaat tttcatgccg
     1321 tcttccaggt cgaacctcac cgcccggacg ccgtaggggc ggaaatacac cgcccgaatc
     1381 tggcttttgt cgtctttgag cgtgaaataa acatgccccg actggggacg gcgcagattg
     1441 gaaacttccc cctccaccca gaggacgcca aaggactcct ccagaaaggt tttaatatta
     1501 tcatttagtt gtgaaaccgt cagaatgtct ttcatccgct ccatcctgct ttgtgaacta
     1561 ccagataatg acggggatga caagtttcaa ttccgcccga acgcccgcgg ctttcggtcc
     1621 gccgcggccc tttttgcacc cttgatacgg gcgggcaaat tccgtttgcc cggcctcaag
     1681 gcctgtgtta aacatttttt tgaggggagg aacatcatgt ccgaaaacta tgatttgatt
     1741 gtgatcggcg gagggccggg gggacttgcg gcggctcaga cggcgtcggc cgcgaacaag
     1801 cgggtgctga tcatcgaaag aggcggctgg ggagggacct gcacgcaccg cggctgcatt
     1861 cccaccaagg cgatgctcgc ctgcagccgg ttttacgcgg atttgaaaaa gctcaagcgc
     1921 gtcggcgtga acgtctccgg cggcgccgtc gattttgccg ccatgaaaaa acaccgcgac
     1981 cagatggtcc gaatcgctgc cctgggagcg cagaaaatga ttgcgggccc gcgggtggaa
     2041 agcaaaattg gacttggaga aattctttcg ccccgggagg tgcgctgcac gggcgaagac
     2101 ggcgtccggc agtttttgaa aacggaaaag attctgatcg cctggggatc gaagccgcag
     2161 attctgccgg gccttcgagc gtcggagcgc attcggacgt ccgatgaaat tctcagtctc
     2221 gaacaattgc cgtccagcat aatcattgtc ggcggcagtt ttatcggcgt tgagtacgcc
     2281 acgctgtttg ccgaactggg cgtccgggtg acgctggtgg agcttatgga ccgcattttg
     2341 ccccgggagg acgaagaagc ggcggagctg ctcggccagg agctggcgcg cctgggggtc
     2401 gttctccata cctcgacccg gctcgttcgc ctggaagaat cgggggaagg cgtcaaactg
     2461 tcggcgagga aaaacggcga agacttggaa atacaggcct tgtacgccct gctgtgcacg
     2521 ggccgggcgc cggttttgca cgccgatcag ctcgactcgc tgggggtgga ctttacgaac
     2581 gcgggaatcc gtgtggacaa aagaatgatg accagtgtcg ggggaattta cgcggcgggc
     2641 gatgtcaccg gcggcatgat gctggcgcac cgggcgtccc tgcaggccaa agttgccgta
     2701 gaaggcatgt tcggcggcag ccgttaccgg tacgatgaag acctcattcc atccgtggtg
     2761 tacagccatc cgcagattgc gcgcgtgggg ctgaccgagc ggcgcgccct ggacgaaggc
     2821 ctggagatca gtgtggtccg gtccgactac ggcgccaatc tgatggcccg cgccgagctg
     2881 atggggcagg ggtttgtcaa aatcattttc catcgggagg tcatcgcggg cgcggtggcg
     2941 gccggcgacc atgcggccga gcttctggcc ccgcttgcgc ttgccgtatc ggggaaatgg
     3001 acgagaaggc aattcaggtc atgggtgctt ccgcatccga cgctcggcga agtctttgcg
     3061 ccgctggcgg actgaacgac agggagaaaa ggcaattcag gtcatgggtg cttccgcatc
     3121 cgacgctcgg cgaagtcttt gcgccgctgg cggactgaac gacagggaga acaggatgaa
     3181 aattattcat tacgcatcgg cggcgccggt gcacgttgac aacgatcagg ccaaaggaat
     3241 cgacgcccgc gtcgttatcg gcaagaatga cggggcggac catttttgca tgcggatctt
     3301 tgagatcgcg ccgggaggat attcaccgaa gcatgcgcat gcctgggagc atgaactctt
     3361 tattcattcc ggcgaggggg aagcttacga cagaggggcc tggcgcccga tttgcccggg
     3421 aacggtgttg tttatcgcgc ccggagagga gcaccagatc cgcaacacgg gagcccggaa
     3481 gctggttttt gcctgcctca tcccgtcggg tgttccggaa ttgtgaggcc gcggtcctgc
     3541 tccccgcagc ggcggggaac aggtgcggca ttggaatttg cttgccaatc ttgaattatt
     3601 tcttgtagct gtaccagcct tcgccggtct tctgtccgaa tttgccttcg gtgactttct
     3661 tcacgacgct gggggaaggc aagtcggccg gattgccgga ctccaggaaa tgactcatgc
     3721 agatgtcgta ggtcagatcg attccggtca ggtcgttgag gcggaacgga cccatcggat
     3781 ggccggctcc gtaaacgcag gccttgtcga tgtcttcgaa agaggccacg cccatctcca
     3841 gcatccacag ggcttcccgc tgaatgacgg cgaaaatccg gttcaggacg aagccgtcca
     3901 cttccttctt gatatggatg ggaattttat cgattttcag gcagaaatcc atcatgcact
     3961 gcgccgtttc gtcggaaaca tgcggcccct gaacgacctc gaccagcttc atgaccagcg
     4021 ccggattgaa gaagtgaaga ttgcagacct gggaagggcg tttggtggca tcggcgatcc
     4081 gggagctcac gataaaggag ctgttggtcg ccaggatcgc tgaaggcgga gcatacttgt
     4141 ccagatcggc aaatactttg cgcttcaggt cgatgcgctc caggacggcc tcgatgacga
     4201 tgtccgcgtc tttggcggct tcctggatgt cgccggtaaa ggatatcagg ctgcgggcct
     4261 ttttcgcagc ttcctccgtc attttcccct tctgcacgcg gcccggcaga tagccttcaa
     4321 caaacttctg ggccttttcg agaacggccg gcacgacatc cgtgctgacg gtcttaaaac
     4381 cggaaagagc gcactggagc gcaatctgat gccccatgtt gcccgccccg acgacgcaaa
     4441 cattttttac atcctggatt ttcattttcg ttcctccttc tttgttttgg cggatttttc
     4501 ctttataaaa cggcggccag gaatcatttt cctacagcca tcgttggttg taacaattat
     4561 tttgccggtg tcgatttgtc aagtaatatt ttcaatttac attgacagaa agaccggttc
     4621 tgttgtaagg aagcttcaat tcagtgaaag gacccaggtg ccatccatga ccagaaaaac
     4681 cgttgcaggc aaggaggaga cgccgagaaa aagcagcgct ttgtttctca agagacagtc
     4741 gcagatcatt aaaaaagcca cgaagctttt catgaagcag ggctatgcgc agacgtccat
     4801 gcgcgaaatt tccaaggcca cgggcatcga cattcggaac ctgtactatt ttatccgaag
     4861 caaggaggag attctttttc tggtcatcga gatgatccac cgccgtgagc tcgagctttt
     4921 cgaaaagcag ggattcacga gcatcgaaga ccccgtgctg cagctcagaa ccgttgtccg
     4981 cgagttcgtc gacgccggct atggatacgg gcaggaaatc cttcttttgt accgggagtc
     5041 gaagtccctt cccaatcgtc tgctcaaggt gattctttcc cgggaaagcc aggtggttgc
     5101 ccgcatcgag gagattctgc agcggggccg ggagaataac gtgtttcgtt tcgaggatgc
     5161 gtcctttacc gccaatatga ttgtgtatga attgtccctc catgcgctgc gcaactggaa
     5221 catgaagcag tattccaagg aggaactcac cgatctgctt gaaaagcatg tgatcaaagc
     5281 ggtgacggcg taacgccggc cccgcggggg tcaaaaagcc gggagtcgcc tcccggcttt
     5341 tttattttgt taagccagat gcttcttctc gatgccgcgc agcgttgcct ccgcatcccg
     5401 gacaatgccc tgcaccatgt cgttgaccga gggcatatcg ttgatccgct gggccacttc
     5461 gccggcggcc atgagcgcct tttcccggtc gcccgcataa accgccttga tggattcaat
     5521 ctcgaactca atgagcgaca tgtccgtggt ggagtaatcg tcgggcgttc ccagaaagac
     5581 gccgggggat ttcttcagcg tgttttgggc gtgctcttcg ctgcggggag ttttcagcca
     5641 ccgggccggt cccacgaagc cccgggcaac cagcgttccc cggtcctggg cggcgaccac
     5701 gccctctttc cagatctgat gaaaatcgct ttccttcgtc gccaggaatc tcgtgcccat
     5761 ctgcgcgccg tcagcgccca gaaccagcgc ggcggccagc gtgcggccgt cgcagaatcc
     5821 gcctgcgccc gcgaccagcg tgttttcgtc ggacacggca tccaccacgg cgggaagcag
     5881 gatcatggaa tggaccggct cccaggaggt atgaaatccg ccttcatggc ccgaagcgac
     5941 aatcacatcc acccccgcct ttttgcagcg cagcgcgcct ttgacggaag gaaccacgtg
     6001 catccacgtg aagcccgcgc ccttgatttt ttctttccag cccaccggat cgccggcgga
     6061 ggtgaagatg accttgaaat tgttcttcat gtcgggattt tcctccctga cccgaatggc
     6121 cgtatcgatc atgatggtcg aataaggctt gacttccgcc gacaccatga cattgattcc
     6181 gaaaatgccg cccttgtcct tgaccaggcg gtaggtgcgc ttcaaaacct tttccatcac
     6241 agtggccatg tcgtcgtcca tgcccgcctc tccgctttcc acgaaagcct tgtaaatcca
     6301 aggctgatcg ttcttgttga aaaggccgct ggtggaaaga agtcccagaa cgccggcatt
     6361 ggcggcggcg acgcaaaggt tgttgttgga aaacgggccc atgcccgcct ggatgatggg
     6421 gtatttgatc ccgatcattt tgcatagtct cgattgaatc ataaaacatc tcctcttgtt
     6481 tttattgggt tgattccggt ctttcttttt acaacgagcg ttgtgatgat taataggtcg
     6541 gggaagtttt gtcaagtaat atttcggttt taggggaagg cttgttaaaa ttccttgaca
     6601 aaacccttcc ggataaataa tacaacaccc gttgtataaa tcatgaagca cggggacggt
     6661 gaagactatg aaaacaggcg cgattcgtgt tccgcgggga acctttcatg tccgggatcg
     6721 gggaaatgcg gacgggtttc cagtggttat gctgcacggc tggccggaaa gctcttattg
     6781 ctgggagggt gtgacggaat ttctggatcc gcaatggcgc gtgattgctc cggatttgcg
     6841 cggtcttggc gacagtgagc gcacgctgga tgtcaaagct tatcagaagg tggagctggc
     6901 caaagatgtg atcgaaatca tcaacgcgct gggcattcag gatttctttc tggccggcca
     6961 tgactggggc ggcatcgtag cgcaggaaat ggcctttctg attcccgacc ggatcaaaaa
     7021 attcgtcatt ctgaacattc ccgtcctcac caacgtcaaa ggcgcggaag aagtcaccaa
     7081 ggccatgatc gccatccggt acattcccta ctggtatcag tactttcaaa tgatgccggg
     7141 cctgcctgag gccatgatca agggcaagga acgcgaatgg gtcagctttt tcttcggcaa
     7201 aaagggccgg gacggcgcca ttccccggga atccatcgac gaatatgtcc gctgctacag
     7261 catcgaaaat acaccggcca ccgcggcttc ctattaccgt tccatggcgc tcgacgcccc
     7321 tcactgggcg ggactggccg gcaagcggtt ttccatgccg tcgctctata tttacggcaa
     7381 tgaagactcc gtcattattc cggccaacat cacccacctg gaagactgct ttgattccat
     7441 caccgtcgaa cagatcaaag cggcccactt tctccaggag gaaaagcccc gggaagtggc
     7501 ggaactgatg aaccgctttt tcaaatgatt cgcgacaccg ggaaggcggg tcgagtccat
     7561 ctttttgaag gagaaaacat catgtccggc atcgaatcca acaaagatca gtttcgggcc
     7621 ctggccgaca atcccggcca ggagccgttt gtcatgctca atctgctgaa gtttaaaaaa
     7681 gagggaggcc gggagtccta cttccgctat atcagggaat ccggaccttt tgtcgaggcc
     7741 gtcggcgcca aagtgctgta cttcggaaaa ccgaaggagc tgctgcaggg gagcgaggac
     7801 tgggatctgc tgatgctggt ctcgtacccg tcgcgcaagg cgtttctgac catggccaac
     7861 aatcccgact atctgaaggt tcatgaattc cgggcccaag gcgtggagcg cgcggtgctc
     7921 tatgcgacgg accccgtaaa attcagggaa atcctgtcgg agtagcagcg ggctttgcaa
     7981 tccaaacggg atgattgaaa attcgcggcg ggagccccgg gcggatcgtt ccggtccgag
     8041 gattcaagtg tgcgcaagga tcccgtggcg gtgcaaactg cgatgaaaac gttcacaggc
     8101 gccctgagct ttagcgggac acacatcaat accctgtaca gaggtgaaat tatgacaaga
     8161 gaagtaagcg tggataccgt gtatgacaat ccgaacaatt tagtcgattt attcgaacag
     8221 agcgttgtcc aatatccaaa caaccggctg ttcggcacca aaaatccggc ctccggccag
     8281 tatgaatggg tgacctacaa gtccgtggcc gaccgggtgg atcgtctgcg cggcgccttg
     8341 aaaaagcttg gactttccaa gggcgataaa gtgggcgtga tcctccacaa ctgcacggaa
     8401 tggtttgtct gcgaacaggc ggtccacggc ctgggaggcg cgctggttcc catgtacctg
     8461 caggaactgc cgaaagtgat cgaatacatc gtccgggatg ccggcgtgaa atttcttttt
     8521 gtcgcgcatg ccggcgtata tgaaaaagtc aaagatttca aaaacagcat tccaacgctt
     8581 caagagatat tcgttgtttt cggcgagggc gaaaattcga tgaaggcact cgaagagatg
     8641 ggcgccaaga atccagcgtc ctccatcaag cctcgctggt cggacctggc caacatcgtc
     8701 tatacctcgg gaaccaccgg cgacccgaaa ggcgtcatgc tttcccatgg aagcctgacg
     8761 ctgtgcgccc gttccggcgt ggacgggttt ggcctggatg agaccatgca tgtggtggcc
     8821 attttgccct gggcgcatgt cttcggcctg gccgccgatc ttcacgatta tatctattgc
     8881 ggcggcggca tcgcgtttgc cgagtcggtg gaaaaactca ttcagaattt tcaggaagtc
     8941 aaacccacag ggctttccgt tgttccccgc atcgtcaaca aggtgtatga cacgatcatg
     9001 cagggcgttg cggccgatcc ggccaaaaag cagttttttg acgcagccgt ggccgaagcg
     9061 gtcaaaaaca gggaccttcc cgaaaaaaca aaagaattca aagattacga cgcgctggtc
     9121 ttttcccagg tccgcaaggt tttcggcggc aatttgaaat tttttgtcac cggcagtgcg
     9181 ctgatcaaac cggaaatcgc gctgtttttc aaggatgtcg gacagccgac ctttgacggc
     9241 tacggactca ccgaaacggg acccacggtc agcatgaact cccccaaata cggcaacaag
     9301 tacggcacgg tcggcaccgt ggacagaaac atgcacgtgg tcatcgataa atcccgcgtg
     9361 ggggaagaca gcctcgacgg ggagatcatc gtttacggcc ctcacgtgat gatggggtac
     9421 cacaacaagc cccagcagac ggcggaggtg atgatgccgg atacatggaa cggctttccc
     9481 ggcattcgaa cgggcgaccg cggctggctg gatgaagaag gtttcctgca catcaccgga
     9541 cgcttcaagg acgaatacaa actggccaac ggcaagtacg tccatccgga aggcatcgaa
     9601 aacgaggcca agctgctgcg ctggattttg aacatcatgc tctacggcga cggcaaggat
     9661 tacaacgtcg ccattgtcgt tccggacttt gccgctcttc aggcggattc gaagatcaag
     9721 ccccttttga aagatacgct cgaagactcc ctgaaagaga aggccctgac ggattatctt
     9781 tccggggaaa tcgtggcgta tctgcgaaaa acctacggcg gctacgaggt gccgcagaag
     9841 ttcctgtttg tcgcggaaga tttcacggtc ggcaacggca tgctgaccca gacgatgaag
     9901 ctcaaacggc cgctggtcat gaagcaatac ggagcaaagc tccaggcgct ctactagccc
     9961 gggcaaagtt cggcacaaac aaaacccccg ctctctctgg agaacggggg ttctttttta
    10021 cccggtgaat cctgtgcgga ttgcgccggc tcgatttacc gagaacaagc tactgaagcg
    10081 gcacgtagcg gatctgcttc ggatccgtgc tcttgctcat ctttttgatc ttctcaaatt
    10141 cctctttgct cttcgggaac gaaatgacgt agtccacctt gctgaactgt cccttgttga
    10201 cgtactgcat gatggtgccg cagttcatca cgccgttttc ctcgatattg aacagttcgt
    10261 tggggaattt gtcgccggcc gtgggcaggg ccttgacgat gttctggcga accaccttgg
    10321 catccagcga ctgcgccgcg ttgacggcac gggcaatgat gtgcatcatg ttgtagttga
    10381 ggcagttttc cgcgttgacc acggatcctt tgtaagccgc attatatctt ttcatgaaag
    10441 aaggcataat cggcaggggc aggcgtttga tgtcgcccgt gctgatcatt ccttccagca
    10501 ggttcagttt atttccgagc gttttggcaa tgtaatccgg cttggcctgg tcgatcagaa
    10561 cgaacccgcc cttgaagccc aggcttctgg cctgttcaat gaccagagcg cttggagccg
    10621 aaggaccgcc cacgaccagg aaatcgggct tggtggcgat cgccgccgtc aactgtgccg
    10681 aaaagtctgt ttccgtgtag taatttgccg gtttgtcggc cagaatcttg ccgccctttt
    10741 tctcccagac cgctttaaag acattgcgcc aggcatctcc gtaagcgccc agggttacca
    10801 ccattgcgcc gtttctccag cccttgtccc aggcgatgat ggagaagttt ttggcgtagg
    10861 cgacaaagtt ggggacggga ttgatgatca tcgggtgctt cttgtccatg atggtatgga
    10921 cgctggagaa ggccgcgatg atgaagttct gttcaggaat ggccatcaaa gcgccgatcg
    10981 tcgtggcaac gggattgaaa acggcaatgg ccttgttttg acttacaaac cgcatcgcgt
    11041 tgttttttgc cagggtcggg tcggcccggt catcgagctt ttccagtttg aaggtgtatt
    11101 ttttgccctg gacggtcacg ccgcccgcgg cattgatttc tttcaccgcc atgtccacgc
    11161 cgttgtagca atcctggccg tattcggcgg ccggtccgct caaaggcccc gtaaatccga
    11221 tcccgatctc cgcggcttgc gcggccgttc cccacaccac aaacgcaaag acgaacatcc
    11281 agcacaacga tttccaactc ttcattgcat tatccccttt cttccctaaa tgattaccgg
    11341 ccgcttccgg ctcggctgat gcttttccct tgcgcatcac gatgatcccc tttgggtcct
    11401 ttagaaaaac cggatcgccg gggatacggg cgatccgggg caggagaatg tccgggcaag
    11461 catcaaaaag ccatgccgcc cttcccgcag gttcaggcag cgcaacgaac atggacccat
    11521 acgtacaacg agcgttggtt gtacatattc agggagcgct gttttgtcaa gcactatttt
    11581 cagcgttttt ttaaaggcgg acggtctttg gagtccgtgg atttgcgagc cggcgaggct
    11641 tatttcgcgg gtttcagccg gttgtccatg ccggtgaagc cgcattccgg ctggtagcag
    11701 gtgacattca gaaattcgca tttctgttgg cagacggggc aggtttgcgg cggcgcagtc
    11761 tgcacgacgg tgtttccgca gttgctgcac ttccaggtgt tttcgggcgg ggtgatcggt
    11821 gtttcggcca tgaccgttgc ctccttgatt ttttttcagt ggtgaatctt taccacagaa
    11881 aaaatccgtt tggcaaccga ataaacttga aaaccgaaag aatttccggt aagcagaaaa
    11941 gcccttcggg cgcaggagac atgcatggaa aagttctcga aaaaaagcga tctggccgga
    12001 ctttcccttt tgggatcggg tgaaaaagcg aagcccggcc gcaagctgga gacgtttccc
    12061 agccattcca aaagacccta taccgtcacc ctgtccacac aggagtttac ctgcctgtgt
    12121 cccatgaccg gtcagccgga ctttgccgga atcaccatac agtatattcc ggacaggaaa
    12181 atcgtagaat cgaaatcgct caaactctac ttctggtcct tccgcaacga aggcgttttt
    12241 cacgagcatc tcaccaacat cattctggac gacctcgtgg ccgcgctttc cccccgctgg
    12301 tgcaaggtga cggcccagtt tgccgttcgc ggcggcatcg ccatttccgt ggacgcggaa
    12361 tatcccgaag ccggtaagtc aaggaatcca aagcgttaaa tgatttccgc actttcaata
    12421 caatttcttt gacaggcagg gtcattgttc gtatactccc ccggcaaaat cgttctttct
    12481 gaagcgcaac aaataaccat cccacggtaa agacaagagg aaacaacatg gcaaaacgcg
    12541 aagacattaa aaaagtgctg attatcggct ccggaccgat tgtgatcggc caggcctgcg
    12601 aattcgacta ctccggaacc caggcctgca aggcgctccg caagctcggc tacaaaatca
    12661 ttctggtcaa ttccaacccg gccacgatca tgaccgaccc cggcatggcc gacgttacgt
    12721 atatcgagcc gctcaacgcc cagacgctga cggagatcat tgaaaatgaa aagcccgacg
    12781 cgattctgcc caatctgggc ggccagaccg gcctcaacct gacctccgaa cttcaccgca
    12841 ggggcattct ggacaaacac ggcgtcaagg tcatcggcgt tcaggtggac gcgatcgaac
    12901 gcggtgaaga ccgcatcgcg ttcaaggagg cgatgaacaa gctggggatt gaaatgccga
    12961 aaagcaagcc cgccacctcc gtggaggaag ccgaaaagat cgccgccgaa atgggctacc
    13021 cggtggtgat ccgcccggcc tacaccatgg gcggcacggg cggcggcatc gtttacaatc
    13081 tggaagaact gcggctgatc gcgagccgcg gcatttccgc cagcctcatc ggccagatcc
    13141 tgatcgaaga atccgtcatc ggctgggaag agctggagct ggaagtcgtc cgcgacgcga
    13201 agaatcacat gatcaccgtc tgcttcatcg aaaacgtcga tgcgatgggc gttcacacgg
    13261 gcgattccta ctgcaccgcg ccgatgctga cgatttccga agagctgcag aagcgccttc
    13321 agaaatattc ctacgacatc gtcgaggcca tccaggtcat cggcggaacc aacgtgcagt
    13381 tcgcgcacga cccgaagacg gaccgcgtgg tcgtcatcga aatcaatccg cgcacctcgc
    13441 gctcgtcggc gctggcctcc aaggccaccg gctttccgat tgcgctcatc tcctccattc
    13501 tggcctccgg catcacgctg gatgaaattc cctactggcg cgaaggcacg ctggataaat
    13561 attcgccgtc gggcgactac gtcgtggtga agttcgcccg ctgggatttt gaaaaattcc
    13621 ccggcgcggt ggacaagctc ggaacgcaga tgcgcgccgt gggtgaagtc atgagcatcg
    13681 gaaagaacta taaggaagcg ctgcagaagg ccatccgctc tctggaaaag aagcgctacg
    13741 gcctgggatt tgtaaaagac tttcacgccc ggccgctcga tgagctgatg gagctgctca
    13801 gcgagccttc cagcgagcgg cagttcatca tgtatgaagc cctgcgtaaa ggcgcgtcga
    13861 ttgacgcttt gcacgccaag acccacatca aaaaatggtt cattgagcag atgaaggagc
    13921 tggtggcgct ggaagaaaaa atcctggcct gcaaaggcaa aaaactgccc gacgccctgc
    13981 tggtggaggc caagaaagac ggattttccg accgctatct ggcccagatt ctggagaaga
    14041 aggaagaaga gatccgccag cagcgcctgg cggcgggact ggccgagtcc tgggacgccg
    14101 tgccggtgag cggcgtcgaa aacgcggcct attactactc cacgtacaac gccccggaca
    14161 aagtaggcgt cagcaaaaac cgcaagatca tggtgctggg cggcggcccc aaccgcatcg
    14221 gccaggggat tgagtttgac tactgctgcg tgcacgcggc ttttgccctg cgcgacgaag
    14281 gcatcgaatc gatcatggtc aactgcaacc ccgaaaccgt ttccaccgac tacgacacgt
    14341 ccaacaagct gtacttcgaa ccgctcaccg tcgaagacgt tttgagcatt tatgagaagg
    14401 aaaaaccgga cggcgtgatc gtccagttcg gcgggcagac gccgctgaac atcgcggcgg
    14461 acctggcgaa agcgggtgtg aaaatcatcg gcacgtcacc ggagacgatc gatctggccg
    14521 aagaccggga ccgcttccgc gccatgatgg acaaactggg catccccatg ccgaaatccg
    14581 ggatggccag caatcttcaa gaggccttga gtgtggcgaa cggcatcggc tatccgctga
    14641 tggttcgccc ctcgtatgtt ctgggcggcc gcggcatgga agtggttcac gacgagcaga
    14701 tgctcaagcg ctatgttgaa gccgccgtgg acgtcactcc cgagcggccc attctgatcg
    14761 acaagtttct ggaaaacgcg atcgaggccg aagcggacgc catttccgac ggcacggacg
    14821 cttttgttcc agccgtaatg gagcatatcg aactggccgg catccactcg ggcgattccg
    14881 cctgcgtgat cccgcccatc agcatcccgg cgcgccacat cgacacgatc aacgactaca
    14941 cccggaagat cgccgtggag ttcggcgtgg tgggcctgat gaacatccag tatgccattg
    15001 ccaatgatgt ggtgtatatt ctggaggcca atccgcgcgc gtcgcgcacc gtaccgctgg
    15061 tttccaaggt ctgcaacatc tctatggcgc ggctcgccac gcagatcatg ctgggcaaaa
    15121 aactgaagga tttgaacctg aagcgccatg ccttccgcca tttcggcgtc aaggaagcgg
    15181 ttttcccgtt caatatgtat caggaagtcg atccgctcct ggggccggag atgcgctcca
    15241 ccggcgaagt gctgggactg gccgattcct tcggactggc gtattacaaa tcccaggaag
    15301 ccaccggcca gagcctgccc gcggaaggca ccgtgctcat taccgtggaa gagaaggaca
    15361 aggggggcat tctggaagtc gcccgcgcgt ttgccaaaat cggcttccgg atcaaagcct
    15421 ccgaaggcac gtataaattt tttgccgcga acggcattgc cgccgaacgt attttgaaaa
    15481 tgcacgaagg ccgtccgaat atcgtggatg ccatcaaaaa cggagagatc caactggtca
    15541 tcaacacacc ggccgggcgc ctcagcaaat acgacgactc ctacatccgt aaggcggcga
    15601 tcaagtacaa gattccctac atcaccaccg ttgccgcagc ggtggccgcg gttaaaggga
    15661 ttgaggcctg ccgccagggc cacggccggg cgaaatccct gcaaagctat cacgcggaga
    15721 ttaaataagg ctgaagactg aagattgaag gctgaaggtt gagaagtagg gaacggtcgc
    15781 ggccgttccc tgcacccttt gtcatccccg acctgatcgg ggatccagta gtgcgtggcg
    15841 ctcgtgacct ttaggttatt agccgcgctt taacgggtct aaagcccgaa ctgcttctac
    15901 agaaggcccg cttcccgcag gatgtcgcgg atacggtcgg cctgatcggc ccggacgagc
    15961 aggcgcgcgt atgcgcccgc ggcgaccagc atcgcggtgt tttccccctg aaaataataa
    16021 tggatgcctt cgccgtccag cagggatttg acaaacgcga tgtcgccggg attgtaggtg
    16081 gaaaaaacct cgaccatttc ctctcgggga acgcttccgt ccccggaatc ttttttatcg
    16141 gtttccatgg cgagcatcat acacgggaaa ggatttttat ggaacccctc aaaggatcgc
    16201 agcgaaaagt tctgcgcgcg caggcgcatc acttgaagcc gctggtcatg gtcggcgcga
    16261 aaggcgtgac ggacggcctg atcggctccg tggatctggc gctcgcagat cacgagctga
    16321 tcaaggtgaa gttcgtcgag tttaaagaag ccaaaaaaga aatagcagcc gaaatcgcgc
    16381 aggcgacaaa aagcgagctg gtcggcctca tcggcaatat cgccatcctt taccggccca
    16441 atcctgatcc ggcaaagaga aaaattcaag tgtcgtaagg gatttgcgcc ggccggatca
    16501 ggcggatggc cggccgccgg caaggcgaag aaaatgggtt gacagataaa atcaatcgca
    16561 ttatatacag cgcgcctgac ggaagggcag caccgctcca caccgtccgg gcactgttca
    16621 tatcttgatg gggaatcgtt caatggtagg acgccggact ctgactccgt taatcaaggt
    16681 tcgaatcctt gttccccagc caaataaaat
//
LOCUS       DAOPAF010000032        16559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3547_length_16559_cov_54.290536, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000032 DAOPAF010000000
VERSION     DAOPAF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16559
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_16559_cov_54.290536"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1620
                     /locus_tag="PLQ77_04755"
     CDS             178..1620
                     /locus_tag="PLQ77_04755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPL10018.1"
                     /translation="MSIRTSYKWFALAVTLLLLIIVGVVFKMLHIQDEVARIEKQRFR
                     SFALAMEMFQSSEDLTRMARSYVSTGNPVYEKLYFEILDIRGGKLPRPAKYAPTYWHL
                     AGIGRGESSGRGENAALKELMRREGFAREELALLRQAQENSDNLVKMEKQAFAAVRGL
                     YDDGRGHFTVRRAPDRNYAVSLLFSERYAHEKARIMEPIRQFMTALDERTNAQSVAYQ
                     SRLRQTILLTLTLAAVTLLVVVLMILYSYRSILRPIERLHEKVSEIARGDYTARCGAT
                     SANEIGELSRNFNNMAGFIETDICMRKQAEERIRHMADHDLLTDLPTMRLARDRLSAA
                     VNMARRHKKAAAVMFADLDGFKAVNDTLGHDAGDDVLRQVAQRLLSCVRETDTVARVG
                     GDEFLVIATEIQIPENAARIAEKIVRCVSEPLAVNGRQAAVGASIGIALFPDDTEDLE
                     QIVQLADKAMYRAKNAGKNRFCFANAGGPE"
     gene            1969..3522
                     /locus_tag="PLQ77_04760"
     CDS             1969..3522
                     /locus_tag="PLQ77_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPL10019.1"
                     /translation="MKEDDVEKKSISRRNFLKTAGTTAAVAGLAASGIPMMAGSAQAA
                     CDELPKKWDETYEVVVVGSGFAGLAAAIEAKNAGASVALLEKMPVHGGNSVINGGDFC
                     APGTKLQKENGVEDSADLLYKDMMRAGMYLNHPELARTVADNAKDALEWTESYLGCRY
                     TKLNFHGGHSVKRAHGTINQSGSEVVNKELAKAKEIGVKIMLRTKLVKFLSDKNGRIV
                     GMEVLKGYRFPDDKTGKTAFIKARKAVILGSGGFSYDVALRQVHDPRLTDKLGSTNHP
                     GATGEALLAACRSGAADVQMDWIQLGPWTSPEEKGFGYVPLFCEPLVGYGLMVNPKTG
                     KRFFKETGNRKERADAILLLGNPAVIMGDSYAVKKQVVPHALKGGLENGAIRKFDSLD
                     DLARAYDMPVGAFKEQVARWNSFAEKRKDDDLGCMIFKDAKPTVEAPFYAARLWPKVH
                     HTMGGLVINKDAQVMGFDFKPVKGLYAAGEVTGGVHGAVRLGSVAMADCIVFGRIAGK
                     NAAKEKAWS"
     gene            3567..4085
                     /locus_tag="PLQ77_04765"
     CDS             3567..4085
                     /locus_tag="PLQ77_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL10020.1"
                     /translation="MEKKKNLIIAAVLFGAVLLMVVSTGLWAAPKLAQSKAKPGDCVA
                     CHVDKKVLPETHPDTRSMDMKACRACHTADNMSLTGKFPGSHTHMLSGVSCEACHGRT
                     GQPEEAPMEKCVACHGPTATLAEKTKDVKPTNPHTSPHYGTELDCNLCHKQHAKSENY
                     CAQCHKFDFKTP"
     gene            complement(4166..4819)
                     /locus_tag="PLQ77_04770"
     CDS             complement(4166..4819)
                     /locus_tag="PLQ77_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL10021.1"
                     /translation="MLKKYRFLLADKLQNIVYYLIRAYSWTFRLRVENEKPWLDHLRG
                     GGRILLCCWHQQFFSAVRYFKNYAPYHPALMISQSRDGDVIARIAEKTGWHTVRGSSS
                     KDGGTALKGMIAHLSQYGFAGHILDGPRGPAGVVKAGVIRLAQVEGTFVVPIYASADR
                     AWYFRSWDRFMLPKPFARVTLRYGDMLDLASKAGEEDFERQRLRLQEIMLPGLHAGI"
     gene            complement(4928..5347)
                     /locus_tag="PLQ77_04775"
     CDS             complement(4928..5347)
                     /locus_tag="PLQ77_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL10022.1"
                     /translation="MASANILLVDDEEDFRFIFTRQVTRILQDLQLEFFEAGDGEEAL
                     ELLRTGVKPSIIILDYTMPRMSGTELLKTIDREHGDLVNVPRLVLSGYIGEDAIREVK
                     SLRCDYMEKSMNTKIFYQRFCRYLTDKLGLPPAEPER"
     gene            5751..6740
                     /gene="rfaE1"
                     /locus_tag="PLQ77_04780"
     CDS             5751..6740
                     /gene="rfaE1"
                     /locus_tag="PLQ77_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPL10023.1"
                     /translation="MSGRIAKKKALEIIKNYPRSGVLVVGDVMVDHFIWGRVSRISPE
                     APVPVVDVQKDSVMLGGSANVLNTIYAMGGRVFVAGVIGSDDIGRGLIRQLEERRIDT
                     NGILLEKGRPTTLKTRIVAHGQQVVRFDHESRMPITRASAARILDYVRSLRGQIGAIV
                     ISDYSKGVVSRELVEGIQAVAAESKIFLCVDPKSSDFGLYSGVHVITPNHHEAQRAAG
                     MEIVNGDDLLKLGEALLAKFDFQALLVTRGEEGMSLFERRRGIRHTHFPAQAKEVYDV
                     TGAGDTVIGVLALSLAACANLKEATALANHAAGIVVGKVGTSTVSREELIGVL"
     gene            6737..7303
                     /locus_tag="PLQ77_04785"
     CDS             6737..7303
                     /locus_tag="PLQ77_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPL10024.1"
                     /translation="MSKPVPAPAVKLIFSVFAPPGKWINDAICRLSVLYGPPDYVSAQ
                     IPFDDTAYYAPEMGQTLVRRFLSMEKLIRPESLPDIKLAANEIESDTRPDSGRRVNID
                     PGYLSQAHLILATGKGYTHRPYLRDGIYADLTLIYQAKKFCSLPWTYPDYGNERLWTM
                     LAAIRSRYLLQLKTAGDGAKPPVLPRKD"
     gene            7306..8184
                     /locus_tag="PLQ77_04790"
     CDS             7306..8184
                     /locus_tag="PLQ77_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPL10025.1"
                     /translation="MIKSMTGYGRVETICDGRNIVVEAKSVNHRFLEIALRTPAALYP
                     LEMEYKKKIGERFRRGRIDVSIRLEGEGAETSRVHLNMEVARDAFEVLSRLRREFNIE
                     EPVSLKNLIGFRDIFSPPAETKLDADFLDRVEQTMREALSLLVRMRQDEGIALFADMH
                     GRLQSIRDIMEAIRLRAPQVVLDYQKRLSDRVRELTAGMEPDPARLAQEVAILADRCD
                     ITEEIVRMGSHIGQFEALLASEEAEGRKIDFLLQEMNREINTIGSKSSDTDIARLVIE
                     VKSELGKLREQAQNIE"
     gene            8189..8812
                     /gene="gmk"
                     /locus_tag="PLQ77_04795"
     CDS             8189..8812
                     /gene="gmk"
                     /locus_tag="PLQ77_04795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPL10026.1"
                     /translation="MKEKGICMVISAPSGSGKSTLCRRLLAACPDMEFSVSFTSREPR
                     PNEINGKDYHFISRENFQCRIERGEFVEWTENFGEFYGTSGKVLQDLLQSGKDLLLDI
                     EPRGASQVKNQLQEVVLVFVLPPSLDELLRRLQRRGHETPEAIQKRFDRAESELKEVL
                     WYDYAVINEDLDAAAKQLTAIYQAEKCRTNRLLGMIRRLYPTMKKEA"
     gene            8827..9096
                     /gene="rpoZ"
                     /locus_tag="PLQ77_04800"
     CDS             8827..9096
                     /gene="rpoZ"
                     /locus_tag="PLQ77_04800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPL10027.1"
                     /translation="MARITIEDSLKVAKTRFGLVSLASKRARQLLKGSRPLMESKNRE
                     IVLALREIAAGKVVYAHPEFLNGSQEDFKPIPDNTEFIGDEEYLE"
     gene            9077..9835
                     /gene="pyrF"
                     /locus_tag="PLQ77_04805"
     CDS             9077..9835
                     /gene="pyrF"
                     /locus_tag="PLQ77_04805"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPL10028.1"
                     /translation="MKNISNSRPEDRLIFALDAPGVEEASSWIDRLSGSVGLFKVGKE
                     LFTSAGPSIVERVLQKGGRVFLDLKFHDIPNTVARAGEAATALKVDMFNVHASGGSRM
                     IRETVQAVGALAEKKGIERPAVLAVTVLTSLNDQDLAEIGFEKTAGDLVLHLAKMARE
                     AGASGVVASPRDIAALRRALGDDFLIVTPGIRASDEKVADDQKRTLSAFEAISAGADY
                     IVVGRPIRKARDPLEACRRIIREIADGLEARNRA"
     gene            9865..10620
                     /gene="rlmB"
                     /locus_tag="PLQ77_04810"
     CDS             9865..10620
                     /gene="rlmB"
                     /locus_tag="PLQ77_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPL10029.1"
                     /translation="MEAVYGINPIKVLLSRDATPLRKIIIAEGRSGPPLQEILTLAGQ
                     KNIPVEWAPRRRLDQLTGRTDHQGVAGLGEAFAYADLEDVLKNRSPRVSQDLVLILDG
                     ILDPQNLGSVIRSAYCLGANGVVIPSDRSASVTPAAMKASAGSARRLPIARVTNLSRT
                     IDELKSRGFWIYGADARGGKSIREQDFSEPAALVMGSEARGLRPLIRKKCDFFLSIPM
                     AGNFDSLNVAVAAGILQYEILLQRLVKSSGSRA"
     gene            10738..11946
                     /locus_tag="PLQ77_04815"
     CDS             10738..11946
                     /locus_tag="PLQ77_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPL10030.1"
                     /translation="MPVFLWEATTKKGDVRKGEMDVADEMAVRTALRRQGFKSVTVKP
                     KPKDLLENIPFLQGGVEEKNVVVFCRIFSTMIDAGLPLIQCLSILAEQEENKTFAKIL
                     RTIKEDIEGGTSLTDALKKYPKVFDDLFVNLIAAGEAGGILDVVLARLSAYLENAMKL
                     KAAVKSAMTYPITVLCIAAGVVTLLLLKVIPTFQKMFADMGGELPGPTQVVVGMSEFM
                     QNYWWVLGVIVAAIVIGLKLFYKTEKGHWTIDAMLLKAPLIGNVLRKVAVAKFTRTLA
                     TMMSSGVPILEGLNIVSKTAGNVVIEAAVVKTRQAISEGQSIAEPLSASGVFPPMVVQ
                     MIAVGEATGALDIMLNKIADFYDDEVDAAVKGMTAMIEPIMMVFLGGVCGGMIIAMYL
                     PIFKMASVVG"
     gene            complement(11952..12028)
                     /locus_tag="PLQ77_04820"
     tRNA            complement(11952..12028)
                     /locus_tag="PLQ77_04820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11992..11994),aa:Pro,seq:cgg)
     gene            12127..12534
                     /locus_tag="PLQ77_04825"
     CDS             12127..12534
                     /locus_tag="PLQ77_04825"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HPL10031.1"
                     /translation="MIIYDIKCENGHTFEGWFKDRQAWIEQNAGRLVCCPVCNSSSVE
                     IAPSSLTIMGKDSKPPRNKDVSRGQVLQSLRRYLENNFEDVGNKFAEVALKIHAGDEE
                     RRNIKGTTTPREETVLKEEGVSFIKISLPKMDS"
     gene            12631..14946
                     /gene="cysS"
                     /locus_tag="PLQ77_04830"
     CDS             12631..14946
                     /gene="cysS"
                     /locus_tag="PLQ77_04830"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPL10032.1"
                     /translation="MMNVYKSIMDRIGNTPLVEISKLNPNKKVTILAKLESANPGGSI
                     KDRTALSMIENAEKRGDLRPGKVILEATSGNTGIGLAMVAAAKGYKICLAMSEAASEE
                     RKKILRAMGAELIFTPAPLGTDGAIEVAYRMLRENPEKYFCTDQFNNPDNVEAHYCGT
                     AQEIWDQTEGRVTAVVVTLGTTGTAMGLSKRLKELNPNIRIIGVEPYLKHKIQGLKNM
                     RESYRPGIFDKKRLDEKVNILDEDAFETARRLAREEGILAGMSSGAALFVAARKAREM
                     EEGLIVVIFPDSGERYLSTELFTVKEELATMSFYNALKRRKTLFRPLKPEEVRLRTCG
                     PTVHDAPHLGNYRRLVVSDLLCRYLAFKGYAVKHVVDIVDCGDKSIRGSEKAGLNLAD
                     YSGRYLRVFLEDVRFLRIRPENIYVKASEDADAALAIVGKLVDRGYAYEKLHSVYFDI
                     SKLGDYGSLSNVDLAKTRTGRSVDLDDYEKDSPADFALLKRAGLSELKRGIYTKTKWG
                     NVRPGWHLECAAISQKYLGAAYDIHVSGADETFPHCENLVAISKACFGNGGAQYWIGA
                     ELVLVDGRKMSRSLNNIATMADLRQSGYGGRDVRFFLLGMNYRKPISYSENALRMAGN
                     TVKKMDTLISRLRAVSHSANDFPEVDQLIYDLNHGFEHALDDDLNISGALAALFDFIG
                     AVNTPLAGGKISRQDASKILEALQKINDVLQIFEFKEQIDDQGIRELIQKREAARQAG
                     LWEEADSLRNRLAGLGVEVLDSPQGTIWHFR"
     gene            14958..15779
                     /gene="amrB"
                     /locus_tag="PLQ77_04835"
     CDS             14958..15779
                     /gene="amrB"
                     /locus_tag="PLQ77_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPL10033.1"
                     /translation="MENIRKSVIAGSWYPGDPAVLRREIEEYFRKVPVREISGEVAGL
                     IAPHAGYRYSGQVAAYAYNLVRGKKYDGVVLIGPSHRVAFPGVSVWGSGAFETPLGLV
                     PVHEALVREISRFDKNIIDFPAAHAQEHSLEIQLPFLQAALGDFSFVPLVMGDQSAAV
                     CRRLAEAVVRAGENRRLLLVASSDLSHFHEDEQARALDGVATRRLRDNDAEGLLEKLA
                     HDQTEACGGGPMAAVMMAAKRLGASHSLLLKYAHSGNVTGDNTSVVGYASAVFYQ"
     gene            15795..16343
                     /gene="amrA"
                     /locus_tag="PLQ77_04840"
     CDS             15795..16343
                     /gene="amrA"
                     /locus_tag="PLQ77_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPL10034.1"
                     /translation="MELTDQEKTALLEIARRAVIAKVANRELPRLSGDLPILKEKRGA
                     FVTLKKRGHLRGCIGYIKAVKSLGETVQEMAVAAAFHDPRFPSVKSEEIRDLKFEISV
                     LSPLRKIQSIDEIEVGNHGLFVVRGYNSGLLLPQVASEYGWDRETFLRETCFKAGLPH
                     KAWKDKETDIYIFSANYFSDRD"
BASE COUNT         3759 a         4357 c         5075 g         3368 t
ORIGIN      
        1 gctggtggtc tatggcgagg aaaagaaatc cggcgcccac ggacatcacg cgtgaaccgc
       61 gcgatcagct tgatcaaaaa aggcccggag gcaaaacctc cgggtttttt ttggcttggc
      121 ctgaactgag tttaatgtta gaaacattcc cgcgagcatt tcaagccggg gaatgtcatg
      181 agcatcagaa catcgtacaa atggtttgcc cttgccgtca cattgctgct cctcatcatc
      241 gttggcgttg tttttaaaat gcttcacatt caggacgaag tcgcgcggat cgaaaaacag
      301 cgtttccggt ccttcgcgct tgccatggaa atgtttcaga gctccgaaga cctgacgcgc
      361 atggcgcgca gttacgtgtc cacgggcaat cccgtttatg agaagcttta cttcgagatt
      421 ctcgacatcc gcggcggcaa gctgccgcgt ccggcgaagt acgcccccac ctactggcat
      481 cttgccggca tcggcagagg cgagtcttcg ggcagggggg aaaacgcagc cttgaaggaa
      541 ttgatgcggc gcgaaggctt tgcccgggag gaactggcgc tcttgaggca ggcgcaggaa
      601 aactcggata acctggtcaa aatggaaaaa caggcctttg ccgccgtcag ggggttgtac
      661 gatgacggcc gcggccattt caccgtgcgg cgcgcgcccg accggaatta tgccgtaagc
      721 ctgctttttt ctgagcggta tgcccacgaa aaagcccgta tcatggagcc gatccggcag
      781 ttcatgacgg cgcttgatga gcgcaccaat gcccaatcgg ttgcttatca atccagactg
      841 cggcaaacca ttctgctgac gctgacgctg gcagccgtca cattactcgt ggtggtcctg
      901 atgattctgt attcctatcg aagcattttg cgtccgatcg aacgtctgca cgaaaaagtt
      961 tccgaaatcg ccaggggcga ctatacagcc cggtgcggcg caacctccgc caacgagatc
     1021 ggtgagttat caaggaactt caataatatg gcgggtttta tcgaaacgga catttgcatg
     1081 cggaagcagg ccgaggagag aatcaggcac atggccgacc atgatctgct gacggatttg
     1141 cccacgatga ggctggcgcg ggaccgtttg tccgctgccg tgaatatggc ccgccggcac
     1201 aagaaagcgg cggccgtcat gtttgcggat ctggacggat tcaaggccgt caatgacacg
     1261 ctcggccatg acgccgggga tgatgtgctg agacaggtgg cgcagcgcct gctctcctgt
     1321 gtgcgagaaa ccgatacggt tgcccgcgtg ggaggggacg agtttctggt catcgcgacg
     1381 gagattcaga tccccgaaaa cgctgccaga attgccgaaa aaatcgtccg gtgcgtgtcg
     1441 gagccgctgg cggtcaacgg gcggcaggcg gccgtcggcg ccagcatcgg gatcgctctt
     1501 tttcccgatg acacggaaga cctggagcag atcgttcaac tggccgacaa ggccatgtat
     1561 cgcgccaaga acgccggcaa aaacaggttt tgcttcgcaa atgccggggg accggaatga
     1621 ggccgcggcg cggggatgga tcttccggcc ggccggttct ttggagaagc cgagaccggt
     1681 cttcttgtcg atatgggaaa ttttccattc gaaggaaagg aagcaaaccg gcgttcgccc
     1741 ggcggaatcc gaaatgaaaa agcgtttgca ttcagctttt ttggaaatgt tggtcattga
     1801 cacatttcct ccggccggtg tatgaatcag tcaggcgatc tttgcatttc ccggtttttc
     1861 attggatggg tgttggttat cgtatacggc taggcgcccg aattgtgccg gtgtgctgat
     1921 gctgtgaggc gctcacggag caggcaattt aaatgaagga ggtatcatat gaaagaggat
     1981 gacgttgaaa aaaagtcgat cagcagacgg aattttttaa agacggccgg cacaacggca
     2041 gcagtcgccg gactggccgc aagcggaatc cccatgatgg cgggaagcgc ccaggcggcc
     2101 tgcgacgagc tgcctaaaaa atgggatgaa acctacgagg tcgtcgtggt gggcagcggc
     2161 tttgccgggc tggctgcggc cattgaagcg aaaaacgcgg gcgccagtgt tgccctgctg
     2221 gaaaaaatgc cggtgcacgg cggcaattcc gtcatcaacg gcggagactt ctgcgctccg
     2281 ggaacgaagc tgcaaaagga aaacggcgta gaggattcgg cggatctcct gtataaagat
     2341 atgatgagag ccggaatgta cctgaatcat ccggagctcg ccaggaccgt tgccgataac
     2401 gcgaaagacg ctctggagtg gaccgagtcc tatctgggct gcagatacac gaaactgaat
     2461 tttcatggcg gccattcggt caaaagagcg catggaacga tcaatcaatc cggttcggaa
     2521 gtggtcaata aggaactcgc gaaagccaaa gagattggcg taaaaattat gctgcgcacc
     2581 aagctggtga aattcctgtc cgataaaaac gggcgaatcg tcggtatgga agtgctcaaa
     2641 gggtaccggt ttccggacga caaaaccggc aaaaccgcct ttattaaagc gcggaaggcg
     2701 gtgattctcg ggtccggcgg tttttcctat gatgtcgcgc tccgtcaggt tcatgatccc
     2761 cggctcacgg ataaactggg atcaaccaac catcccgggg ccaccggcga agctctgctg
     2821 gccgcctgca ggagcggagc ggcggatgtt cagatggatt ggattcagct ggggccatgg
     2881 accagtccgg aagaaaaggg ttttggctat gttcctctgt tctgtgaacc gctggtcggt
     2941 tacggtctga tggtgaatcc caaaaccggg aagcgttttt tcaaggaaac cggaaaccgc
     3001 aaggaacgcg cggatgccat tcttctcttg ggaaatcccg ccgtgatcat gggcgattct
     3061 tacgcggtca aaaagcaggt cgtccctcat gcgctcaagg gaggcctgga aaacggagcg
     3121 atcaggaaat ttgactcgct ggacgatctt gccagggcct acgacatgcc cgtgggcgct
     3181 ttcaaggaac aggttgcccg ctggaattcc ttcgcggaaa aaagaaagga tgacgatttg
     3241 gggtgcatga tctttaaaga cgccaaaccg accgtcgaag cgcctttcta tgccgcgcgc
     3301 ctgtggccga aagttcatca caccatgggc ggactggtga tcaataaaga cgcccaggtg
     3361 atgggattcg acttcaaacc ggtaaaaggt ctttatgccg ccggagaagt gacgggaggc
     3421 gtccatggcg ccgtgcggct tggaagcgtc gcgatggcgg attgcattgt cttcggccgc
     3481 atcgccggca aaaacgcggc caaagaaaaa gcctggagtt gaggttgcgt ttcagcgcgg
     3541 atttgatgaa gcaaggagaa gatcgtatgg aaaaaaagaa aaatctgata atcgcggcgg
     3601 tcctgttcgg cgcggtttta ctgatggttg tcagcacagg actctgggcg gcgcccaagc
     3661 tcgcccaatc caaagccaag ccgggcgact gtgttgcctg tcatgtcgat aaaaaggtgc
     3721 ttccggagac gcatcccgac acccggtcga tggatatgaa ggcctgccgg gcctgccaca
     3781 ccgcggacaa tatgagcctg accggaaaat ttcccggcag ccacacgcat atgctctccg
     3841 gcgtgagctg cgaagcctgc cacggcagga ccgggcagcc ggaagaggcg ccgatggaga
     3901 aatgcgtcgc ctgccacggg ccgacggcaa cgctcgcgga aaagacaaaa gatgtgaagc
     3961 cgaccaatcc gcatacatcc ccgcattacg gaacggagct ggactgcaac ctgtgccaca
     4021 aacagcatgc caaatcggaa aactactgcg cgcagtgcca caagttcgac ttcaagacac
     4081 cgtaaaatga cccctggatt ttcaccccaa accggcggcg ggtcccatga ccgccgccgg
     4141 ttttttgcaa agtgtttttc acggatcaaa ttccggcatg caggcccggc agcatgatct
     4201 cctggagtct caatctctgg cgttcaaaat cttcttcgcc tgcctttgaa gcgagatcca
     4261 gcatgtcccc ataccgcaga gtaacgcggg caaagggttt gggcagcatg aagcggtccc
     4321 agcttctgaa ataccaggcc cggtcggcgg aggcgtaaat ggggaccacg aaagttccct
     4381 caacctgggc caggcggatt acgccggctt tgaccactcc cgcaggtcct ctcggtccgt
     4441 ccaggatatg gccggcaaag ccgtattgcg acaggtgcgc gatcatgcct ttcaacgccg
     4501 tccccccgtc tttcgacgac gaaccgcgga cggtatgcca gccggtcttc tcggcaatgc
     4561 gggcgatgac gtcgccgtcc ctgctctggc tgatcatcag cgccgggtga tagggcgcat
     4621 aattcttgaa gtagcggacg gctgaaaaga actgctgatg ccagcagcac agaagaatcc
     4681 tgccgccgcc ccgcaggtga tcgagccagg gcttttcgtt ttccacgcga agccggaacg
     4741 tccagctgta ggcgcgaatc agataataaa caatattctg aagcttgtcc gccagcaaaa
     4801 atctgtattt tttcagcatc aggatgttcc ctgcgagatg acgttccatg gcaccggttg
     4861 actgcgccgc atggcgcggg atggcgtttc ggcctgagtc ggaatcagcc ggtccgtttg
     4921 cgatttttta ccgttccggt tcagcggggg gaagccccag cttatcggtg agatacctgc
     4981 agaaccgctg ataaaagatt ttcgtgttca tgctcttttc catataatcg catcgcagac
     5041 tcttcacttc cctgatggcg tcctcaccga tatatccgct caggacgagg cggggaacgt
     5101 tgaccaagtc gccatgttcg cggtcgatgg ttttgagcag ctccgttccg ctcattctgg
     5161 gcatcgtata atcaagaatg atgatggagg gcttgacgcc cgttcggagc agttccagcg
     5221 cctcttcgcc gtccccggct tcaaaaaact ccagttgcag gtcctgaagg atgcgtgtca
     5281 cctgccgggt gaaaataaag cggaaatctt cttcgtcgtc caccaggagg atatttgcac
     5341 ttgccataac tgccctttgc cttttgctgc cctgtttcat ccggacgttg gccggtccgc
     5401 ggaatggatt ccgcccgcgc ctgccgccaa agcttatccg ccgatgccgt gcaaaaacat
     5461 tgcatatttt ctgacattcc gcaacgcgtg acttgccctt acggctgatg aaggggcccc
     5521 cgagaaaatc atgacgaaaa tcattgacaa atccggctcc cggtaatagc attccgtcac
     5581 ttttttcctg aatggcgccg cagccgcgga agagtttttg aagtgctgcg acggaatcgg
     5641 gcattcggcg gacggacggg gatgcgaaag ggatatgttc cgctccttcc ggcgtccgga
     5701 tcggaaatca aaacccgtaa aacggcgggg gcggggagca ttgcagaccg gtgagcggaa
     5761 gaatcgctaa aaaaaaggca ttggaaatca tcaaaaatta cccgcgttcc ggcgtgctgg
     5821 tcgtcgggga cgtcatggtg gatcatttca tctggggcag ggtgtcgcgc atttctcccg
     5881 aggcgccggt gccggtggtc gatgttcaga aggattccgt gatgctgggc ggctccgcca
     5941 acgtcctcaa cacgatctac gcgatgggcg gcagggtctt tgtggccggc gtgatcggct
     6001 ccgacgacat cggccggggt ctgatccgcc agcttgagga gcgcaggatt gacacgaatg
     6061 gcattctgtt ggaaaaggga aggccgacca ccctcaagac cagaattgtg gcccacgggc
     6121 agcaggtggt tcggtttgat cacgaaagcc ggatgccgat cacccgcgca agcgcggccc
     6181 ggattctcga ctatgtccga tccctgcgcg ggcaaatcgg agcgatcgtg atttccgact
     6241 acagcaaagg cgtggtttcc cgcgaactgg tggagggcat tcaggccgtc gccgccgaat
     6301 cgaaaatttt tctatgcgtc gatccgaagt ccagcgactt cggcctttat tcgggcgtcc
     6361 atgtcatcac gcccaatcat catgaggccc agcgagccgc cggcatggaa atcgtcaacg
     6421 gagacgatct tctgaaactg ggggaggcgc tcttggccaa atttgatttt caggcgctcc
     6481 tggtaacgcg cggcgaagag gggatgagcc tttttgagcg acgccgcggg atccgccaca
     6541 cccactttcc ggcgcaggcc aaggaagtct atgatgtaac gggggccggc gacacggtca
     6601 tcggcgttct ggcgctgtct ctggccgcct gcgcgaattt gaaggaggcc accgcgctgg
     6661 ccaaccacgc ggcgggcatt gtcgtgggca aggtcggaac gtccaccgtg tcgcgggaag
     6721 aattgatcgg cgtattatga gcaaaccggt tccggccccg gcggtgaagc tgatcttcag
     6781 cgttttcgcg ccgccgggaa aatggattaa cgacgcgatt tgccgcctgt cggtcctgta
     6841 cggaccgccg gactatgtga gcgcgcagat cccttttgat gatacggcgt attatgcgcc
     6901 ggaaatggga cagacgctgg tgcggcgttt tctgtcgatg gaaaagctga tccggccgga
     6961 gtcgctgccg gacatcaagc tggccgcgaa tgaaatcgaa agcgatacgc gcccggatag
     7021 cggccgccgc gtgaacattg atcccggcta cctgtcgcag gcgcacctta ttctggccac
     7081 gggcaaaggc tacacgcacc ggccttattt gcgcgacgga atttacgcgg atttgaccct
     7141 gatctatcag gccaaaaagt tttgttccct gccctggacg tatccggatt acggaaacga
     7201 gaggctctgg acgatgttag cggcgatccg gtcccgctat ctgctgcagc tgaaaacggc
     7261 cggagacggg gcaaagcccc ctgttttgcc gcgaaaggat tgaacatgat taaaagcatg
     7321 accggctacg gccgggtgga aacgatctgc gacggccgga acatcgtagt ggaagcaaaa
     7381 tccgtgaatc accggtttct ggagatcgct cttcgaacgc ccgccgcgct ctatccgctg
     7441 gagatggagt ataaaaagaa aatcggggag cgattcaggc gcggccgcat cgatgtttcc
     7501 atccggctgg agggagaagg cgccgaaacc tccagggtgc atctcaatat ggaagttgcc
     7561 cgggacgcct ttgaggttct aagccggctc agaagagagt tcaatattga agagcccgtg
     7621 agcctcaaga acctgatcgg ctttcgggac attttttcgc cgcccgcaga aacgaagctg
     7681 gacgccgatt ttctggatcg ggtcgagcag acgatgcgcg aagccctgtc gctcctggtc
     7741 cgtatgcgcc aggatgaggg gatcgccctg tttgcggata tgcacggcag gcttcagtcc
     7801 attcgggaca tcatggaggc cattcgcctg cgggcgccgc aggtggtgct ggactatcag
     7861 aagcgtcttt ccgatcgcgt ccgggagctg acggcgggaa tggagccgga tccggcccgt
     7921 ctggcgcagg aagtggccat tctggcggac cggtgcgaca tcacggagga aattgtccgc
     7981 atgggaagcc acatcggtca gtttgaggcc ctgctggcaa gcgaggaggc cgaaggccgc
     8041 aagatcgact ttcttcttca ggaaatgaac cgggaaatca acacgatcgg ttccaagagc
     8101 agcgacacgg acattgcccg cctggtgatc gaggtgaaaa gcgagctggg caaattgcgc
     8161 gaacaggcgc agaacatcga gtaggcgtat gaaggaaaag ggaatctgca tggtcatctc
     8221 cgcgccctcc gggtccggaa aaagcaccct ctgccggagg ctgctcgccg cctgtccgga
     8281 catggaattt tccgtttcct tcacgtcgcg ggagccgcgc cccaacgaga tcaacgggaa
     8341 agattaccat tttatttccc gcgagaattt tcagtgccgg atcgaacggg gcgagtttgt
     8401 cgaatggacg gaaaatttcg gcgaattcta cggaacgtcc ggcaaagtgc tgcaggatct
     8461 gctgcaaagc ggaaaggatc tgctcctcga tattgaaccg cgcggggcca gccaggtcaa
     8521 gaatcaattg caggaggtcg ttctggtttt tgtgctgccc ccctctctgg acgaactcct
     8581 aagaaggctg cagaggcggg ggcatgaaac gccggaggcg atccaaaaac gttttgaccg
     8641 ggccgaaagt gaactcaagg aagttctgtg gtatgattac gccgtcatca acgaagacct
     8701 ggacgcggcc gcaaaacagc tgaccgcgat ttaccaggcg gaaaaatgca gaacaaaccg
     8761 tttgctcggc atgatccgcc gtttatatcc aacgatgaaa aaagaggcat aggaggtgct
     8821 cgattcatgg caagaattac gatagaagat tccctaaagg tggcgaaaac ccggttcggc
     8881 ctggtgtcgc tggcgtccaa gcgcgcgcgc cagctgctga aagggtccag gcccttgatg
     8941 gagagcaaaa accgggagat tgttcttgcg cttcgggaaa tcgcggcggg caaggttgtt
     9001 tacgcccatc ctgaattttt gaacgggtcg caggaagatt tcaaaccgat accggacaac
     9061 acggaattta tcggcgatga agaatatctc gaatagccgg ccggaagacc ggctgatctt
     9121 tgcgctggat gcgccggggg ttgaggaggc ttcgtcctgg attgaccgtc tttccggcag
     9181 cgtcggcctg ttcaaggtcg gcaaggaact gttcacgtcc gcagggccgt ccatcgtgga
     9241 gcgcgttttg caaaaaggcg gacgggtttt tctggatctg aagtttcacg atattcccaa
     9301 cacggtggcc cgcgccggtg aagccgcgac ggcgctgaag gtggatatgt tcaacgtgca
     9361 tgcctcgggc ggaagccgga tgattcggga aaccgtccag gccgtggggg cgcttgccga
     9421 aaaaaaaggc attgaacgcc cggcggtgct ggccgtgacg gtgctgacca gtttgaacga
     9481 tcaggacctg gcggaaatcg gttttgaaaa aacggcgggc gatctggttc tgcatctggc
     9541 aaaaatggcc cgtgaggccg gagcgtcggg ggtggtggca tcgcccaggg atattgccgc
     9601 gctgaggcgg gcgctggggg acgattttct gatcgtgacg cccggcatcc gcgcatccga
     9661 tgaaaaagtt gcggatgatc agaaaagaac gctaagcgcc tttgaagcga tttcggcagg
     9721 cgccgattac attgtggtgg gcagacccat ccgtaaggcg cgcgatcccc tggaggcctg
     9781 ccgccggatc attcgggaga ttgccgacgg actggaagcc cgaaaccgcg cgtgacccta
     9841 tcaaaggcag acggaaggcg gatcatggag gcagtttacg gcatcaatcc catcaaagtt
     9901 ctcctttccc gggacgcaac tcctctgaga aaaatcatca ttgccgaagg ccggagcggt
     9961 ccgcctcttc aggaaatcct aaccctggcc gggcaaaaga acatccccgt cgaatgggcg
    10021 ccccgccgcc gcctggatca actgacgggc cggaccgatc atcagggagt agcggggctc
    10081 ggcgaagcgt ttgcgtatgc cgatctcgaa gatgtgctga agaaccgcag cccgcgcgtg
    10141 agccaagatc tcgttttgat tctcgacggc attctggatc cgcagaatct gggatcggtg
    10201 attcgcagcg cctattgcct gggcgccaac ggcgtcgtca ttccgagcga ccggtcggcg
    10261 tccgtcacgc ccgcggccat gaaggcctcc gccggaagcg cccggcgcct gcccatcgcc
    10321 cgcgtgacga atctgtcccg gacgatcgat gaattgaaaa gcagaggttt ctggatttac
    10381 ggggcggatg cccgcggggg aaaaagcatt cgggagcagg atttcagcga gccggcggcc
    10441 ctggtcatgg gaagcgaagc ccgggggctc aggcctttga tccggaagaa atgcgatttt
    10501 ttcctgtcga ttccgatggc ggggaatttc gactctttga acgtcgccgt ggcggcgggg
    10561 atccttcagt atgaaatcct gcttcagcgc ctggtgaaat cctccggttc ccgggcgtga
    10621 agaaggacaa agcattgcgg gcgtgttcga atttaaccgg ttgaaactgc atggaagatg
    10681 gtttatgatg cagccataaa aaaagactcc tgatcaagaa gaaaggaagt ttgcgctatg
    10741 ccggttttcc tttgggaagc gacgacgaag aagggcgatg tcagaaaagg cgagatggat
    10801 gtcgccgatg agatggccgt ccgcacggcg ctgcggcggc agggattcaa atccgtcacg
    10861 gtcaagccaa agcccaaaga ccttctggaa aacattccct ttcttcaggg gggcgtggaa
    10921 gaaaaaaacg tggtggtgtt ctgccggatt ttctccacga tgatcgacgc cggcctgccg
    10981 ctgatccagt gcctgagcat tctggccgag caggaggaaa acaagacgtt tgccaaaatt
    11041 ctccggacga tcaaggaaga catcgaaggg ggaaccagcc tgacggacgc gctgaaaaag
    11101 tatccgaagg tctttgacga cctgtttgtc aacctgatcg ccgccggcga agcgggcggc
    11161 attctggacg tcgttctggc gcgcctttcc gcgtatctgg aaaacgcgat gaaactcaag
    11221 gcagccgtca aaagcgccat gacctacccc attaccgtgt tgtgcattgc cgccggcgtg
    11281 gtgacgctgc tgcttttgaa ggtcattccc acctttcaga agatgtttgc cgacatgggc
    11341 ggcgagcttc ccggtccgac gcaggtagtc gtggggatga gcgaattcat gcagaattac
    11401 tggtgggtgc tcggggtgat cgttgccgcg atcgtcatcg gcctgaaatt gttttacaaa
    11461 acggaaaaag ggcactggac gattgacgcg atgctgctga aggcgccgct gatcggcaat
    11521 gtgctccgga aagtggccgt cgccaaattc acgaggacgc tggccacgat gatgagctcc
    11581 ggcgtgccga ttctggaggg attgaacatc gtcagcaaaa ccgccgggaa cgtcgtcatt
    11641 gaggcggccg tggtgaaaac gcgccaggcc atcagtgaag gccagtccat cgccgaaccg
    11701 ctgtccgcgt ccggcgtttt tccgccgatg gtggtgcaga tgatcgcggt gggggaagcc
    11761 accggcgcgc tggacatcat gctcaacaag atcgccgact tttatgacga cgaagtggac
    11821 gcggcggtca agggcatgac ggcgatgatc gagccgatca tgatggtttt cctgggaggg
    11881 gtttgcggcg gcatgattat cgccatgtat ctgcccatct tcaagatggc gtccgtcgtg
    11941 gggtaaaaaa atggtcggga tggcgcgatt tgaacgcgcg actcctgcgt cccgaacgca
    12001 gtgccctacc aagctgggcc acatcccgac acgggaagcg acgttataaa atttatcgcg
    12061 aaatgtccac atgtttttat ttccaacgga tttttagccg tttttaacaa ggaaagcgcc
    12121 cgagcggtga ttatttacga catcaaatgt gaaaacggac acacctttga aggctggttt
    12181 aaagaccgcc aggcctggat agaacagaat gccgggcgtc tggtctgctg tcccgtctgc
    12241 aacagttcca gcgtggaaat cgcgccttct tccctgacga ttatggggaa ggattccaag
    12301 ccgccccgga acaaggacgt ttcccggggg caggtgcttc agtcgttgcg ccgctacctt
    12361 gaaaacaact ttgaagatgt cggaaacaaa tttgccgaag ttgcgctgaa gatccacgcg
    12421 ggcgatgaag agaggcgcaa tatcaaagga acgacgaccc cccgggaaga aacggtcttg
    12481 aaagaagaag gggtttcgtt tataaaaatt tctctgccca agatggacag ctgatccgtt
    12541 atatttcctc cgggatgact ttttcaggcg cctgcgcaat ccttgtttca aaaaggaatg
    12601 atgaaggatt gacgaacgga gcgtagggtg atgatgaacg tgtataaaag cattatggat
    12661 cgcatcggca acacgccgct ggtggaaatc tcgaagctga atccgaataa aaaggtcacg
    12721 attctggcca agctggagtc cgcgaatccg ggcgggtcca tcaaagaccg cacggcgctc
    12781 agcatgattg aaaacgccga aaaaagaggt gatctgaggc ccgggaaggt gatcctggag
    12841 gccaccagcg gcaataccgg catcggactg gccatggtgg ctgccgcaaa gggctacaag
    12901 atttgtctgg ccatgtccga ggcggccagc gaagagcgca aaaagattct gcgggcgatg
    12961 ggcgccgagc tgatctttac ccccgcgccg ctggggacgg acggcgccat cgaagtcgcc
    13021 taccggatgc ttcgggaaaa tccggaaaag tatttctgca ccgaccagtt caacaacccg
    13081 gacaacgtcg aggctcatta ttgcgggacg gcgcaggaaa tctgggacca gacggagggc
    13141 cgggtcaccg ccgtggtggt cacgctcggg acgaccggca ccgccatggg cctttccaaa
    13201 cggctcaagg aactgaatcc gaacattcga atcatcggcg tcgaaccgta cctgaagcac
    13261 aagattcagg gcctgaaaaa catgcgggag tcctaccgcc ccggcatctt cgacaaaaag
    13321 cgcctggacg aaaaagtgaa catcctggac gaagacgcct ttgagacggc ccgccggctg
    13381 gcccgggaag aaggcattct cgccggcatg agttccggag cggccctgtt tgtcgcggcc
    13441 cggaaagccc gcgagatgga agaagggctg atcgtggtga ttttcccgga cagcggggag
    13501 cgctatctga gcacggaatt gttcacggtc aaagaagagc tggcgacgat gagcttttac
    13561 aatgcgctca aacgccggaa aacgcttttc cgtccgctga aaccggagga ggtgcggctg
    13621 cgcacgtgcg gcccgaccgt tcatgacgct ccccatctgg gaaattaccg ccgcctggtg
    13681 gtatccgacc tgctgtgccg ctacctggcg ttcaagggct acgccgtcaa gcatgtcgtc
    13741 gatatcgtgg actgcggcga caagtcgatt cgggggtcgg aaaaggcggg actgaatctc
    13801 gcggattatt ccggccggta tttgcgggtg tttctggagg atgtccggtt tttgcggatc
    13861 cgtccggaaa atatttacgt caaagcctcc gaagacgccg acgccgcgct tgcgattgtc
    13921 ggcaagctcg tcgatcgggg ctacgcctat gagaagctgc actccgttta tttcgacatt
    13981 tccaagctgg gagactacgg cagtctgtcc aatgtcgatc tggccaaaac ccgcaccggc
    14041 cggtcggtgg atctcgatga ttacgaaaag gacagcccgg cggacttcgc ccttttgaaa
    14101 agagccggcc tgtccgagct caaaaggggg atttacacca agacgaaatg gggcaatgtg
    14161 cggccgggct ggcatctgga atgcgcggcc atttcgcaga aatacctggg cgcggcctat
    14221 gacatccacg tcagcggggc ggatgaaacg tttccgcact gcgagaatct tgtcgcgatc
    14281 agcaaggcat gtttcgggaa cggcggagcg cagtactgga tcggggccga actggttctg
    14341 gtggacggca ggaaaatgtc ccgctccctg aacaatattg cgacgatggc cgaccttcgg
    14401 caaagcgggt acggcggccg ggatgtccgt tttttcctgc tgggcatgaa ttaccgcaaa
    14461 ccgatcagct attcggaaaa cgccctgagg atggccggaa acactgtgaa aaagatggac
    14521 acgctcattt cccgcctcag ggcagtgagc cattccgcca atgattttcc ggaggtggac
    14581 cagctgattt atgatcttaa ccacggcttt gaacatgcac tggatgacga cctcaacatt
    14641 tccggcgctc tggcggcgct tttcgatttc atcggcgccg tcaacacgcc cctggccggg
    14701 gggaaaatca gcaggcagga tgccagtaag atcctggagg cgcttcaaaa aatcaatgat
    14761 gttcttcaaa tatttgaatt caaagagcaa attgacgatc aggggataag ggaactgatt
    14821 caaaaaaggg aggccgcaag acaggcgggc ctctgggagg aggcggattc gttgagaaac
    14881 cggctggccg ggctcggcgt tgaggttctc gacagcccgc aggggaccat ctggcatttc
    14941 cggtagggga aaggcaaatg gagaatattc gaaaatccgt catcgcgggt tcatggtacc
    15001 ccggggaccc cgccgttttg aggcgcgaaa tcgaagaata tttccgcaag gttcccgtcc
    15061 gggagatttc cggggaagtg gccggtctga ttgcgcctca tgccggctac cggtattccg
    15121 gccaggtggc ggcatacgcg tataacctgg tccggggaaa aaaatacgac ggggtcgttc
    15181 tcatcgggcc ctcccaccgg gttgcttttc cgggtgtttc cgtctggggc agcggagctt
    15241 ttgaaacccc cctgggcctg gttccggtcc atgaggcgct ggtgcgggaa atcagtcgtt
    15301 tcgacaagaa tatcattgat tttccggcgg cgcacgctca ggagcattcg ctggaaattc
    15361 agcttccttt cctgcaggct gctctgggcg atttctcctt tgttccgctg gtgatgggag
    15421 accagagtgc cgcggtctgc cgaaggctgg cggaagcggt cgtacgggct ggcgaaaacc
    15481 ggcgcctgct gctggtggcc agcagcgatc tgtcgcattt tcacgaggat gagcaggcca
    15541 gagcgctgga cggcgtcgcg acgaggcgcc tgcgggacaa cgacgcggaa gggcttctgg
    15601 aaaaactggc gcacgaccaa accgaagcct gcggcggcgg tccgatggcc gctgtcatga
    15661 tggcggcaaa gagactgggc gcttctcatt cgctcctttt gaagtacgcc cactcaggga
    15721 acgtgaccgg ggataataca tccgtggtcg gctacgcttc tgcggttttt taccaataaa
    15781 aaaaagaggt tgatatggag ctgacagatc aggaaaaaac ggcgcttttg gaaattgcca
    15841 gaagggcggt catcgccaag gtcgcaaacc gcgaattgcc aaggctctcc ggggatttgc
    15901 cgattctgaa ggaaaaaaga ggcgcgttcg tcaccctgaa gaaaaggggg catctgcgcg
    15961 gctgcatcgg gtacatcaag gcggtcaagt ccctggggga aacggtgcag gaaatggcgg
    16021 tcgccgcggc ttttcacgat cctcgctttc cgtccgtgaa aagcgaagaa atccgtgatt
    16081 taaaatttga aatatcggtg ctgagtcctc tcagaaagat tcagagcatt gacgaaatag
    16141 aggtcggaaa tcacggcctg ttcgtcgtca ggggctataa ttccggtttg ctgctgccgc
    16201 aggtggcctc cgagtacggc tgggaccggg aaacgtttct gcgcgagacg tgttttaaag
    16261 cgggtctgcc ccacaaggca tggaaagaca aggaaacgga tatttatatt ttctccgcga
    16321 attatttcag cgaccgggat tgaattttcc ccgtttcgtc aacggaagaa ttttccaaat
    16381 aatttaaaaa tttacttgac aaacgcgaat ataaaaatta gtgtcctcaa ttcgcacgaa
    16441 gcggtgttgt cgcttttttg cgtttaaagc ggcggcggct ttatgtgaaa acgtaatgat
    16501 tcagatggtt tgctggcgta gctcaatcgg tagagcagct gatttgtaat cagcaggtt
//
LOCUS       DAOPAF010000033        15960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3730_length_15960_cov_49.879346, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000033 DAOPAF010000000
VERSION     DAOPAF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_15960_cov_49.879346"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1325)
                     /locus_tag="PLQ77_04845"
     CDS             complement(75..1325)
                     /locus_tag="PLQ77_04845"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPL10035.1"
                     /translation="MKIGILTIGNELMNGRIADTNSSFIAREINLQGWTVETMMSVGD
                     DFEIIDNRLHYLLSFTDAVICTGGLGPTSDDITTAAVARAFGRQLVLDEKVLTYLKDI
                     FTRFNLRWTGNNAKQALFPEGAEVIPNPVGTAAGFALPVGEKLVMVIPGVPSETRRMV
                     PEGVIPVLRRRFPQAVLQTVKQTIKTFGLGEAMVDQKLADVDFDALGVSVGFYPVFPE
                     NHLVLITHSDSLEQAQKNMQQAVDKVTVRLSDCIFSFGEESLEERIAAELTAKKLTIA
                     VAESCTGGLIADRLTDVSGSSVYFERGLVTYSNEAKIQLLGVPADILEKHGAVSEETA
                     RAMAEGVRKLAGTNLGLSSTGIAGPTGGTKEKPVGTVYIALTDGEKTVCRHYAYRWDR
                     RRNKLISSEAALMMLRNYLQETPS"
     gene            complement(1335..1799)
                     /locus_tag="PLQ77_04850"
     CDS             complement(1335..1799)
                     /locus_tag="PLQ77_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPL10036.1"
                     /translation="MTTSQKDKWIRILATGFGTGLSPVAPGTAGTLAGVLICLASSPL
                     PWLFRLFFVVAISAVSIYVAGEAEKLYGNTDDQRIVIDEIAGYQVAMLPVAVTGLHLL
                     LAFLLFRIFDIWKPFPLKHFQKFPGGWGVVVDDLGAGVYAGILMLIFVFLTP"
     gene            1972..2463
                     /gene="mobB"
                     /locus_tag="PLQ77_04855"
     CDS             1972..2463
                     /gene="mobB"
                     /locus_tag="PLQ77_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPL10037.1"
                     /translation="MIPIVSIVGKSNSGKTTLLEKIIADLTHRGYRIATIKHNRHGFD
                     IDHEGKDSYRHKKAGAHTTVVSSPHQLALVQDVDHDHSFEEIRDKFISGADIILTEGF
                     KVNDYPKIEVFRTELKRDLISSRKDGLVAVAADVKLDIDVPCLDINDPKTVADFIEIH
                     FLK"
     gene            complement(2597..3505)
                     /locus_tag="PLQ77_04860"
     CDS             complement(2597..3505)
                     /locus_tag="PLQ77_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPL10038.1"
                     /translation="MKKMMWLKAVVLAVLLLLPLALTAPVQAARSIDPIVSTEWLQNN
                     LNNANLVIVDVRKVEEYKAGHIPGAVNVVYGTWAVKKGDLLNELPAVDDLADAIGSAG
                     IDQNSLVVIVGKTDKVPDQFDMTRVAWTLKYLGVPNVAILSGGQNQWLKESKPVLQDM
                     TRPKEKEFAASVNKGLFVDKASLAGKIGKALVIDTRAPAFYEGKEKLPFVPKLGRIKG
                     AVNLPVGQLYTPEGLYKDKAALAALAQKAAGSDLDKEIIVYCDTGKTCTGWAFIMTDL
                     LGYKDVKVYDGSSMEWLADPNVPTEP"
     gene            complement(3571..4290)
                     /locus_tag="PLQ77_04865"
     CDS             complement(3571..4290)
                     /locus_tag="PLQ77_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPL10039.1"
                     /translation="MTEKILSEKNRVVRHSRLELIEHWAIAISGLILLATGIFELPVA
                     KRYYIVTVPGLRWTADFITSLYLHYAAALVFTAAGIFHLAYHGMRGETGLLPKKGDLS
                     ASVTVIKSLFGFGQEPPMHKYLPEQRLAYAGMAWIIILLIVSGLIKTYKNLYAPDLSL
                     AVVLTATWIHNICFVLFFLAFLAHMAAILLRPNWPMVRGIFTGKVRLDYARHRHALWI
                     ADLEKTKEPLIPDRDLRCGKV"
     gene            complement(4283..5287)
                     /locus_tag="PLQ77_04870"
     CDS             complement(4283..5287)
                     /locus_tag="PLQ77_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10040.1"
                     /translation="MDSGKASKITRRSFLRISAGAAVLTGVHVPKTARAESAGSLSTL
                     IDLSLCDGCADRNMPACVQACRSINANKIPQVAENIPEPWPRKTIEDWSKKQGVFNRL
                     TPYNYLYVHRADVMVDGRSQTLFVPRRCMHCDNPACATICPFSANHKNKNGAVVIDPD
                     QCFGGAKCRDVCPWEIPQRQSGVGIYLHVLPDFMGNGVMYKCDLCNDRLKEGKKPGCI
                     EACPRGAMLIGPRKEIEAEAQARAHQINGHIYGQKENGGTATLYVSPVSFELLNQTMT
                     KKPGQPDMKPGVKRRMAGTDPLGKAVLASPVLGLALAGAAGWFSRRKERQARQEEDRD
                     "
     gene            complement(5300..5593)
                     /locus_tag="PLQ77_04875"
     CDS             complement(5300..5593)
                     /locus_tag="PLQ77_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPL10041.1"
                     /translation="MFIYTPTGYIMDEMRIKVLNRLKRIEGQIRGLERMVEQEAPCAD
                     VLTQLNAASAAMKKTGVVIIQANMRRCIQEAADDQEKSIEEFQNALTRFIGMS"
     gene            5704..7047
                     /locus_tag="PLQ77_04880"
     CDS             5704..7047
                     /locus_tag="PLQ77_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPL10042.1"
                     /translation="MDPACDLKNIYRDAVREVDPAGLILSRIRKEGRKLALTGQEAEW
                     SEDLSSYRQVMVLGIGKASARMASAMESLLDEDLTSGFVITKYGHGLDLRKVKVHEAG
                     HPVPDENSLKGARILTDMARAADEHTLIIHLISGGGSSLFCLPADGISLDHKRRTTQA
                     LLASGAGIDEVNCVRKHLSKVKGGGLARIASPARLINLILSDVIGDRIDTIASGITAP
                     DPTTFQDALSIVHRYGLDGRLPEAVMDRLTRGARGEVAETPKAGNPAFCNSINLILGN
                     NALACGAAREAAQKLGYDAQILSTTLSGEAVEAGKCFAQLAKAIKEGKSLKEKPAIII
                     AGGETTVTLRGKGKGGRNQEMALAFALELHRILPDSSNIFFLSAGTDGGDGPTDAAGA
                     LVTPALMEKMKNISLEAASSLQDNDSYSFFQKEGGLFITGPTYTNVCDIQILAVT"
     gene            7203..8372
                     /locus_tag="PLQ77_04885"
     CDS             7203..8372
                     /locus_tag="PLQ77_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPL10043.1"
                     /translation="MNTYIDYSKRIRKIQEEMKKLDIDLFVGTRTVSLSYVAGAFVPW
                     RSAVIVSADGYAGLITMLLDCERLKNESWLSSISPYAPLPGMDLLDLIVHFVTQNKAQ
                     KGRIGVELGHSPRGNTGYLFATEYEFLKKALPDATFVNAVNVVDRACYIKEPGEIKLL
                     RQAAAIADSGIKCVRDALDVGMMETEIAGIGEMELRRLGSEYHWAVTGSSEVASGYRA
                     TYAMNGTTQPTQKMVQEGENIIVDLHPLYQLYPSDLAHNFILGKPSSEQRKLADAYLK
                     TAETIVRNFKAGNKVGDISKTVMDTVTELGYAPYTIPSFGHGLGVFGHEWYPAIVNND
                     EFRDVVLEENVVEVAFLAMTVPGVCGMRLECPVLVTKDGGEMLCKTPLALTVLEV"
     gene            complement(8634..8969)
                     /gene="trxA"
                     /locus_tag="PLQ77_04890"
     CDS             complement(8634..8969)
                     /gene="trxA"
                     /locus_tag="PLQ77_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPL10044.1"
                     /translation="MGDKANVKHSDDQHFSSTVLKQDGPFLVDFWAPWCGPCRALSPV
                     LEELADLYEGKIDIVKVNVDENPETASKYGVRSIPTLLLIQNGNVRETQIGLLSRQQL
                     AAMIDRNLN"
     gene            9099..9776
                     /locus_tag="PLQ77_04895"
     CDS             9099..9776
                     /locus_tag="PLQ77_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPL10045.1"
                     /translation="MDILERMKKIDLFQGVSFESLRVLAAQALYKKYAPQEMVIGEAD
                     FIRSFYIVLEGRLKLYKSSPEGREQTIQLLEPGDPFGLCTAFATDAFPASAMAMEPSG
                     VLLIPGTLVEAVALKEPRLLLNIIQILSRRLQHSMALIESLALKEIPARLASFVLHSL
                     LQRPEGAARLDLVITQRELAKILGATPEALSRALRKMTDEGILRASGRTIHVLNRSAL
                     QRLAEGE"
     gene            9944..10840
                     /locus_tag="PLQ77_04900"
     CDS             9944..10840
                     /locus_tag="PLQ77_04900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPL10046.1"
                     /translation="MKRIFLPQFVILALFIVLLPAAGLARDITPIVSTDWLLTNLKNP
                     KLVVLDIRRVEDYREGHIPGALNAFYGAWAYMKDGLYASLPEKDEIDDTISYMGINFD
                     RWVVVTGCMDTPRLSYQSARVACTLQYAGIKNVALLDGGTNKWIAEKKPLSTRVEKRK
                     AGKFKGKYTSEKFADKNYVKERLGKLILLDLREREFFTGEKKMDCVPMRGHIPGAFNM
                     PTSCAFNEDRTFKTKEELTAIAEAAAGKDLNAEIVTYCDAGQCCPTWSYLLTEVLGYT
                     NVRLYIGSMQEWMQDPKAPVER"
     gene            10921..11097
                     /locus_tag="PLQ77_04905"
     CDS             10921..11097
                     /locus_tag="PLQ77_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10047.1"
                     /translation="MKNRRTFLLAFLAVLIAALYGTAVFAATPNKCIQCHTNDALMKS
                     LHKPPVLPKSDGEG"
     gene            11131..12168
                     /locus_tag="PLQ77_04910"
     CDS             11131..12168
                     /locus_tag="PLQ77_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL10048.1"
                     /translation="MYKGYVVDKALLDKDPHFKAGCVLCHAGNDGELGKEKAHLGMLK
                     KPSDNLRSCGVCHKKVAANYAKSLHYTTIGQRTGVMSRFSAAELKTFDEKVFEKSCRS
                     CHASCGDCHVKGAPVGGISIGLVAKHKFVKKDEGKTCAFCHGGRVYPEYTGDYGGAPD
                     VHYQKGMLCMDCHKKEEFHGDGTAYKSKNEIPQRPACKSCHPNGKEAKMETQVAHRQH
                     EGKVSCYGCHSGSAYRNCNDCHGGHAAASPGMILGRSPRDKKVLTTLRLIPTVRDTFA
                     PAGIKMEHFDAVPNYWDTPAHNIKKRTDRTRKCDACHVDRKNFLTKETMIKNGAKANE
                     DLIFVPKAIKN"
     gene            complement(12336..12563)
                     /locus_tag="PLQ77_04915"
     CDS             complement(12336..12563)
                     /locus_tag="PLQ77_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPL10049.1"
                     /translation="MTTGNDFAVVLFESVSHALGAEKIVKGSGIGCKLIHVPRHLSSD
                     CGICLRFHAADRERVEALLRGKLHFFEIKLL"
     gene            complement(12560..13735)
                     /locus_tag="PLQ77_04920"
     CDS             complement(12560..13735)
                     /locus_tag="PLQ77_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPL10050.1"
                     /translation="MKTIYLDNAATSFPKPEAMLSAMTAYQRNVGANPGRSGHGLSID
                     AGRIICEARESLALLFHVEDPLRIVLTKNSTEALNIALQGGLRPGDHVITSGMEHNSV
                     MRPLRFLEGRGVELSVVPCSPRGEMDPEDLKKVLRKNTRIVVVTHASNVTGTILPVEA
                     IGELLQDRPDVMFCVDAAQTAGALPIDVEKMNIDLLAFTGHKSLFGPQGTGGLYIREG
                     LDQRIAPLMMGGTGSRSEFEEQPEFMPDRYESGTPNTIGIAGLGAGVSFVLEQTVEAV
                     RAREEELASRFLAGLQDLRDEVILYGPQDASRQTAVVSFNIKNVTSSDAADYFEEQRG
                     ILCRPGLHCAPSAHRTIGTFPRGTVRFSFGFFNTQQEVDDACEALRELVKRKGKPGS"
     gene            complement(13763..13975)
                     /locus_tag="PLQ77_04925"
     CDS             complement(13763..13975)
                     /locus_tag="PLQ77_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPL10051.1"
                     /translation="MSTKKVDARGLSCPQPVVLVDRAIADGNKDLEILVDNEVARENV
                     SRLLSRRGLKADIRQDGSDIIIRAAK"
     gene            complement(13997..15076)
                     /gene="yedE"
                     /locus_tag="PLQ77_04930"
     CDS             complement(13997..15076)
                     /gene="yedE"
                     /locus_tag="PLQ77_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPL10052.1"
                     /translation="MKNIFSTRWGIILVGAVIGIGAALLQYFGNPPNMGICVACFERD
                     IAGALGLHRADVVQYLRPEIMGFVLGAFVAALLAGEYKPRGGSSPLIRFFLGVFAMIG
                     ALVFLGCPWRTLLRLAGGDGNAILGLAGLIAGIFIGVGFLKNGYSLGRSYAQKKAAGW
                     VFPALMIGLLLLLVFQVSFAPGGPIFFSAKGPGSQHAPLLISLIAGLVIGGLAQRSRF
                     CTMGAFRDVILIRDFHLISGVAALLVFAFIANMLLGQFKPGFEGQPVAHTDHLWNFLG
                     MTLAGLSFVLAGGCPGRQLFLSGEGDMDAAIFATGMIVGAAIAHNFAIASSPKGIAAF
                     GPAAVLIGFLFCLVVGFTQRDKVSA"
     gene            15335..>15960
                     /locus_tag="PLQ77_04935"
     CDS             15335..>15960
                     /locus_tag="PLQ77_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL10053.1"
                     /translation="MALIWVNHISISFGGPLLLDGASLQIEAGERIGLLGRNGSGKST
                     LMKLLSGEIPPDDGEISRDKNIHVSVMPQEVPDLRGTVYDVVAGGCQEHLDLLHEYHD
                     LTLRLHSGGDHQLLKKIEQVQHRLEAVGAWQYHQKVEEVISRVSLDESSEFLLLSAGL
                     KRRVLLARALVADPDVLLLDEPTNHLDIDAILWLEEFLKSFEKTLMFVT"
BASE COUNT         3628 a         4523 c         4396 g         3413 t
ORIGIN      
        1 gcgggtcttc agacccgcga tacacagcgc gcctgaaggc acgcgctgca ttttcccttt
       61 gtgacgattt gtcgtcaaga gggtgtctcc tgtaaataat ttctcagcat catcagcgcc
      121 gcctcggagg agatcagctt gtttctcctg cggtcccagc ggtaggcgta atgacggcaa
      181 accgttttct ctccgtcggt aagcgcgatg taaaccgttc cgaccggctt ttctttcgtg
      241 ccgccggtcg gtcccgcaat gccggtggag gaaaggccca gatttgtccc cgccagtttg
      301 cggacgccct cggccattgc ccgcgcggtt tcttcgctca cggcgccgtg cttttccagg
      361 atgtcggccg gcacgcccag cagttgaatt tttgcttcat tgctgtaggt cacgaggccg
      421 cgctcaaaat aaacggaact gccggaaacg tcggtgaggc ggtcggcaat gagccctccc
      481 gtgcaggatt ccgccacggc gatcgtcaat ttctttgccg tcaactccgc cgcgattctc
      541 tcttcaagag actcttcacc gaaggaaaag atgcagtcgg aaagtcgaac cgttaccttg
      601 tccacggcct gttgcatatt tttctgcgcc tgctccaggg aatcggagtg tgtgatcagt
      661 accagatgat tttccggaaa gacggggtaa aagccgacgc tcacgccgag ggcgtcaaaa
      721 tcaacatcgg ccagcttttg atccaccatg gcctctccga ggccgaaggt tttaatggtc
      781 tgtttgaccg tctgaaggac ggcctgcgga aagcggcggc gcaaaacggg aatgacgcct
      841 tccgggacca ttcgtcgcgt ttccgacgga acgccgggaa tgaccatcac cagcttttcc
      901 ccgaccggca gcgcaaatcc cgcggcggtg ccgacaggat tgggaatgac ttcagcgcct
      961 tcgggaaaca gcgcctgctt ggcgttgttt cccgtccagc gcaggttgaa tcgcgtaaag
     1021 atatccttaa gataggtcag gactttttca tccagaacca gttgccggcc gaacgcccgc
     1081 gcgaccgcgg cggtggtgat gtcgtcggaa gtcggtccca ggcctccggt gcagatcacc
     1141 gcgtccgtaa aagacaaaag gtaatgcagc cggttgtcga tgatttcaaa gtcgtcgccg
     1201 accgacatca tggtttcaac cgtccagccc tgcagattta tctcccgcgc gatgaaagac
     1261 gaattcgtat cggcgatgcg cccgttcatt aattcgttcc cgatggtcaa aatgccgatt
     1321 ttcataaaac cgccttatgg tgtaagaaaa acaaaaatca acatcagaat tcccgcgtaa
     1381 acgcccgcgc cgagatcatc taccaccacg ccccagccgc ccggaaactt ctgaaaatgc
     1441 ttcagcggaa aaggcttcca gatatcaaaa atccgaaaaa gaagaaacgc cagcaaaaga
     1501 tgcaggccgg taacggccac aggcagcatg gcaacctggt agccggcaat ttcatcaatc
     1561 acaatccgct ggtcgtcggt gtttccgtaa agtttttccg cctccccggc aacataaata
     1621 gagacggccg atattgcaac cacaaagaaa aggcgaaaaa gccagggcag agggctggaa
     1681 gccaggcaga tcaaaacacc cgccagcgtg cccgccgttc ccggcgcgac gggggaaagg
     1741 cccgtcccga atccggttgc caggatcctg atccatttgt ccttttgaga ggttgtcacg
     1801 gacgtccttt ttaatcccgt tgtttgcgtg tagaacgatt gttctatact acaggccttt
     1861 gcgggatgtc aagcaatctt atgcacggcc taggcctttg gccgacattg ttggccaaag
     1921 ccggcaaaat gtggtatgca acggccaagt aaaggaattt caggataaaa aatgattccc
     1981 attgtttcca ttgtcggcaa gtccaattcc ggcaagacaa cgcttctgga gaaaatcatt
     2041 gccgatttaa cccatcgggg ctatcgcatc gccaccatca agcacaaccg ccacggcttc
     2101 gacatcgacc acgagggaaa ggacagctac cgccacaaaa aggcgggagc gcacaccacc
     2161 gtcgtttcat cgccccatca gctcgcgctg gttcaggacg tggaccatga tcactccttt
     2221 gaggaaattc gcgacaagtt catttccggc gcggatatca tcctcaccga aggattcaag
     2281 gtgaatgact atccgaagat tgaagtgttc cgaactgaac tcaaacggga cttgatctcg
     2341 agcagaaaag acggtctggt cgcggtggcc gcggacgtga aactggatat cgatgttccc
     2401 tgtctggaca tcaacgaccc caaaactgtc gccgatttca ttgaaattca ttttctgaaa
     2461 tagccataaa gcttgcagaa cttacaaaca atgctctcaa tcaatctatt gcggcgtttt
     2521 gtctttgccg aaaaaaagcc cggcggtttg acggccgggc tttcgtttgc ttcaggggat
     2581 gcagcgtgag gatgttctag ggttcggtag gaacgttcgg gtccgccagc cattccatcg
     2641 aggaaccgtc atacactttg acgtccttgt atcccagcag gtcggtcatg ataaaggccc
     2701 agccggtgca ggtcttgccc gtgtcacagt aaacgattat ttccttgtcc aaatcggagc
     2761 cggccgcttt ttgcgcaagc gccgcaagcg ccgccttgtc cttgtacaga ccttccggcg
     2821 tgtagagctg gccgaccggc aaattgaccg ctcccttgat gcggcctaat ttcggaacaa
     2881 agggcagctt ttccttgcct tcgtaaaacg ccggagcgcg ggtgtcgata acgagcgcct
     2941 tgccgatttt acccgcgagc gaagccttgt ccacgaacag acccttgttg acggacgccg
     3001 caaattcttt ttctttgggg cgggtcatgt cctgcaaaac cggtttgctt tccttcagcc
     3061 actgattctg cccgccgctg agaatggcga cattcggcac gcccagatac ttcagggtcc
     3121 aggccacgcg ggtcatgtcg aactgatcgg gaaccttatc cgttttcccg acaatgacca
     3181 ccagagagtt ctgatcgatg cccgcgcttc cgatcgcgtc ggccaggtcg tccacagccg
     3241 gcagttcgtt aagcaggtcg ccttttttaa cggcccaggt tccgtacaca acgttgacgg
     3301 ctcccggaat atggcctgcc ttgtattctt ccaccttccg cacatcaacg atgaccaaat
     3361 tggcattgtt caggttattc tgcagccact ccgtggagac gatgggatca atgctcctcg
     3421 ccgcctgtac aggagcggtg agcgccaacg gcagcaacaa cagtaccgcc agaaccactg
     3481 ctttcaacca catcattttt ttcataaata ctacctcctc tgtttcattt gaatttttgt
     3541 ctgtcgctat tttgaagcct gtgacggccc tcaaaccttc ccacagcgaa gatcccggtc
     3601 agggatcaac ggctctttcg ttttttcaag atcggcgatc cacaaagcat ggcggtgccg
     3661 ggcatagtcc aaacggactt tgccggtgaa gatgccgcgg accatcggcc agttcggcct
     3721 aagcagaatc gccgccatgt gcgcgagaaa agccagaaaa aagagaacga aacaaatatt
     3781 atgaatccag gtggccgtca ggacgaccgc aagcgaaaga tccggcgcat acaggttctt
     3841 gtaggttttg atcaggccgg agacgatgag caggatgatg atccaggcca ttcccgcgta
     3901 agccagccgc tgctccggca aatacttgtg catcggcggc tcctggccga agccgaacag
     3961 gctcttgatg actgtgacgg acgccgacag gtcgcctttt ttcggcagca ggccggtttc
     4021 gccgcgcatt ccatggtacg ccaggtgaaa gattcccgcg gccgtaaaaa ccagcgccgc
     4081 cgcgtagtgt agataaaggg atgtgatgaa atccgccgtc cagcgcaagc ccggaacggt
     4141 gacgatgtaa tagcgtttgg ctacgggcag ttcaaaaatt ccggtggcca gcagaatcag
     4201 gccggaaatc gcgatggccc agtgttcgat cagctccagc cggctgtggc ggacgacccg
     4261 gtttttttcc gaaaggatct tttcagtcac ggtcttcctc ctgacgggct tggcgttctt
     4321 tgcggcgtga aaaccagccc gccgcgccgg cgagcgccaa tccaagaacg ggggaagcca
     4381 gcacggcttt tcccagcgga tccgttcccg ccatgcgccg cttgacgccc ggcttcatgt
     4441 ccggctgccc gggttttttg gtcatggttt gattcaacag ctcgaacgat acaggagaca
     4501 catagagcgt tgccgtgccg ccgttttctt tctgaccgta aatgtggccg ttaatctggt
     4561 gggctctggc ctgcgcctca gcttcaatct cttttctggg gccgatcagc atggccccgc
     4621 gcggacaggc ttcgatgcat cccggctttt ttccttcctt taacctgtca ttgcagaggt
     4681 cgcatttata catgacgccg ttgcccatga aatcgggcag gacatgaagg taaatgccga
     4741 cgccggactg gcgctgcgga atttcccacg ggcagacgtc cctgcatttg gcgccgccga
     4801 aacactggtc tgggtcgata accaccgcgc cgttcttatt tttgtgattg gcggaaaacg
     4861 gacagatggt cgcgcaggcg ggattgtcgc agtgcatgca gcgcctcggg acaaacaggg
     4921 tttggctgcg gccgtccacc attacatccg cccgatgaac gtaaaggtag ttgtaggggg
     4981 taaggcggtt gaagacgccc tgctttttgg accagtcttc gatggttttt ctgggccacg
     5041 gctccggaat gttttccgct acctgtggga ttttgttggc gttgatgctt cggcaggcct
     5101 ggacgcaggc cggcatgttg cggtccgcgc agccgtcgca aaggctcaaa tcgataagcg
     5161 tcgagagaga tccggccgat tcagcccgcg cggtctttgg gacatgaact cccgtcagaa
     5221 ccgcggctcc ggccgaaatc cgcaaaaaag aacggcgggt gattttagaa gcttttcccg
     5281 aatccaattt ttcccccctc taggacatgc cgataaagcg ggtcagcgcg ttttgaaatt
     5341 cttcaatgct cttttcctga tcatcggccg cctcctgaat gcagcgcctc atgttggcct
     5401 gaatgatcac cacgccggtt tttttcatgg cggcgctggc cgcgttgagc tgggtcagga
     5461 catcggcgca gggcgcttcc tgctccacca tccgctccag cccgcggatt tgcccctcaa
     5521 ttcgctttaa tcggtttaat accttgattc tcatttcgtc cataatatac cccgtggggg
     5581 tatatataaa cactcccggg tttcctgtca aatggttttt ttacactgat ttttgaaatc
     5641 acagcttgac aatggaagcc cccttcatgg tagctgtttg aaaaatatcg cgtggagaaa
     5701 gccgtggatc ctgcttgcga cctgaaaaac atttaccgcg acgccgttcg ggaggtggac
     5761 cccgcgggcc ttattttatc ccgcatccgg aaagagggcc ggaagcttgc gctcacgggc
     5821 caggaagcgg aatggtcgga agacctttcc tcctaccggc aggtgatggt gctcggcatc
     5881 ggcaaggctt cagcccgcat ggcatccgcc atggaaagcc tcctggacga agatctcact
     5941 tcgggattcg tcatcacaaa atacggccac gggctcgatc ttcggaaagt gaaagtccat
     6001 gaagcgggcc accccgtgcc ggatgaaaac agcctgaaag gagcccgcat tctgacggat
     6061 atggcccggg cggcggatga acacacgctg atcatccatc tgatttccgg cggagggtca
     6121 tccttgttct gcctgccagc ggacggcatc agcctggacc acaagcgcag aaccacgcaa
     6181 gccctgctgg cgtccggcgc cggcatcgac gaggtcaact gcgttcggaa gcatctgtcg
     6241 aaagtcaagg gcggggggct cgcccgaatc gcgagtcccg ccaggctcat caacctgatt
     6301 ttgtcggatg tgatcggcga ccggatcgat accatcgcgt ccggcattac cgctcctgat
     6361 cccaccacgt ttcaggacgc cctctcgatc gttcatcgat acggactgga cggcaggctt
     6421 ccggaagcgg tgatggaccg tctgacccgg ggggcgaggg gcgaagtggc cgagacgccc
     6481 aaagcgggca acccggcatt ttgcaacagc atcaatctga tccttggcaa caacgcgctg
     6541 gcctgcggtg cggcgaggga agccgcgcag aagctgggat atgacgcgca aattctcagc
     6601 acaacgctgt ccggcgaggc ggtcgaagcg ggaaagtgct tcgcgcagct tgccaaggcc
     6661 ataaaggaag gaaaatccct gaaggaaaaa ccggccatca taatcgccgg cggagaaaca
     6721 accgtcacgc tccggggcaa agggaaagga gggcgaaacc aggaaatggc actcgccttt
     6781 gctttggagc tgcaccgaat ccttccggat tcttccaata tcttttttct gtccgccggc
     6841 acggacggcg gcgacgggcc tacggacgcg gcgggcgcgc tggtgacgcc cgcgctgatg
     6901 gaaaaaatga aaaacatctc cctcgaggcc gcgtcgagcc ttcaggataa cgactcgtat
     6961 tctttctttc agaaggaagg cggtcttttt atcacgggtc cgacctatac caacgtctgc
     7021 gatatccaga ttctcgccgt gacatgatgc cgggcgcgcg gcaaaaggcg cggacggacg
     7081 gcgcaggcga atgctgatcc ttcctgccct acccgggttt gcttgacttg acccggcaac
     7141 cggcctatac ttttttggaa cgaaagctga tttcaatcag cggacaaaga aacggaggtt
     7201 ctatgaatac gtacatcgat tattccaagc gcatcagaaa aatacaggaa gaaatgaaaa
     7261 aattggatat tgaccttttc gtgggcacgc gaacggtcag cctgagctat gtcgcagggg
     7321 ccttcgttcc ctggagaagc gctgtgatcg tgtccgcgga tggatatgcc ggtctgatta
     7381 ccatgctgct cgactgcgag cgtctgaaga atgagtcctg gctttccagc atttctccct
     7441 atgctcccct gcccggcatg gatctgctcg acctgatcgt ccattttgtc acacaaaaca
     7501 aggcacaaaa gggcaggatc ggcgtggagc tcggtcattc cccccgcggc aacacggggt
     7561 atctctttgc gacggaatac gaatttttga aaaaagccct gccggacgcc acgtttgtca
     7621 acgcggtcaa cgttgtggat cgggcctgct acatcaagga accgggcgaa atcaagctgc
     7681 tgcgacaggc cgccgccatc gcggattccg gaatcaagtg cgtgcgcgac gccctggacg
     7741 tcggcatgat ggaaacggaa atcgccggca tcggcgaaat ggagctgagg cgcctcggca
     7801 gcgaatacca ctgggccgtc acgggatcgt cggaggtggc ctccggatac cgggcgacat
     7861 acgccatgaa cggaacgacg cagcccacgc agaaaatggt gcaggagggc gaaaacatta
     7921 tcgtcgatct gcacccgctc taccagctct atccctcgga cctggctcac aatttcattt
     7981 tgggaaagcc ctcgtccgag cagaggaagc tggccgacgc gtatctcaag acggcggaaa
     8041 cgatcgtccg caatttcaag gccggaaata aagtcggcga tatcagcaag accgtgatgg
     8101 ataccgtgac cgaactgggt tacgccccat acacgattcc ctcgttcggc cacggcctgg
     8161 gcgtgttcgg ccatgaatgg tatccggcca tcgtcaataa tgacgaattc cgtgatgtcg
     8221 ttctggagga gaatgtggtc gaagtggctt ttctggccat gaccgttccc ggcgtgtgcg
     8281 gaatgaggct ggagtgcccg gttctggtca cgaaggacgg tggcgagatg ctgtgcaaaa
     8341 cgccgcttgc gctgacggtt ctggaagttt gaggtccgca aagagatgac ggaaaagccc
     8401 cggccttttt acccgcgaag gccggggctt ttgtttgaaa gaatccgcta ccgcggagac
     8461 gttttctttt ttctgtccgg agccggacag gagggcctca ttcaggtgga gatgccgaat
     8521 ttcggcaaca gccacgcctg gagaaaaacc catactccta caatcagcag taaaatcaac
     8581 cagtcattca tgtttttcct cctttcgccg ggttggcggc cggttccaac cggctaattc
     8641 aggttgcgat cgatcatagc cgcaagctgc tgcctggaca acaggccgat ctgcgtttcc
     8701 cgaacgtttc cgttttggat caagagcagc gtcggaatgc tccgcacgcc gtactttgac
     8761 gccgtttccg gattttcatc cacattcacc ttgacgatgt cgatttttcc ttcatacagg
     8821 tccgccagtt cttccagtac cggactcaac gcccggcacg gaccgcacca gggcgcccag
     8881 aagtccacca ggaacggccc gtcctgcttt aacaccgttg atgaaaaatg ctgatcatcc
     8941 gaatgtttca cgtttgcttt atctcccatc gtttttcccc cgctaattta ttttgtttca
     9001 tctttggtct gtggtaaacc attcttgcgt tttgcacaaa ccatagcatc ggccggtcag
     9061 cgcgtcattg atctgaatca atgccggggg agcatcgaat ggatattttg gagcgcatga
     9121 aaaaaatcga tctttttcag ggcgtttctt tcgaaagctt gcgggtcctg gccgcgcagg
     9181 ccctgtacaa aaaatacgcc ccccaggaaa tggtgatcgg cgaggcggat ttcatcaggt
     9241 ctttctacat tgtccttgag ggcagactga agctttacaa gagttcgccg gaaggtcggg
     9301 agcagacgat tcagcttctg gagccggggg accctttcgg cctgtgcacc gcgtttgcca
     9361 cggatgcttt cccggccagc gccatggcga tggaacctag cggcgttctg ctgattcccg
     9421 gaacgctggt cgaggccgtg gccctgaagg agcccagact ccttctcaac atcattcaga
     9481 ttctgtcccg ccggctgcag cattccatgg cgctcatcga atcgctggcg ttgaaggaaa
     9541 ttcccgcccg gctcgcctcg tttgtgctgc attccctgtt gcagcgtccg gaaggcgcag
     9601 cccggctgga tctggtgatc acccagcgcg agctggccaa gatcctcggg gccacgccgg
     9661 aagcgctttc acgcgccctg cgcaagatga cggatgaagg cattctccgc gcgtcgggac
     9721 ggacgattca cgttctgaat cgaagtgccc tgcaacgtct ggccgaagga gaatagcgac
     9781 acgctgatgc caaagacggc ttttctgtaa gggataagag ccctgaagag aacattgaac
     9841 tggcaatttt gaacccggac atggtatact ttacacgacg taaagacgaa acggttttga
     9901 cgatacgctc atcccgagcg cgggttgaca aggaggaaat cgcatgaaaa gaatattttt
     9961 gccccaattc gtcattcttg cattgttcat cgttttgctc ccggccgcgg gtctggcgag
    10021 ggacattacc ccgattgttt ccaccgactg gcttttgacc aatctgaaaa accccaaact
    10081 cgttgttctg gatatccgcc gcgtggaaga ctaccgcgaa ggccacattc ccggggcgct
    10141 gaacgctttt tatggcgcct gggcctacat gaaggacggc ctgtacgcgt cgctgccgga
    10201 gaaagacgaa attgacgata cgatcagtta catgggtatc aattttgacc gttgggtcgt
    10261 tgtcacagga tgtatggaca cgccacgcct cagctatcag agcgcccgcg tcgcctgtac
    10321 gctgcaatac gccggcatca aaaatgtcgc gcttctggac ggaggcacca acaagtggat
    10381 tgcggaaaaa aagcccctct ccacccgcgt ggaaaagcgc aaggccggca aattcaaggg
    10441 aaaatatacc tcggaaaaat ttgccgacaa aaactatgtc aaagagaggc tgggcaaact
    10501 catcctcctg gaccttcggg aacgcgaatt ttttacgggg gagaaaaaga tggactgcgt
    10561 tcccatgagg gggcacattc ccggcgcatt caatatgccg acctcctgcg ctttcaatga
    10621 ggacaggacc tttaaaacga aagaagaatt gaccgccatc gccgaagcgg ccgcgggcaa
    10681 ggatctaaac gccgagattg tcacttactg cgacgcgggc cagtgctgcc cgacctggtc
    10741 gtatcttctg acggaagtgc tggggtacac aaacgtccgg ctttacatcg gatcgatgca
    10801 ggagtggatg caggatccga aggcgccggt ggagcgctga tccacgcccc cgatgaatcc
    10861 gtttgacagg ctgaagctga tttgttacat cttgctccac tcttctcttg aggaggtgtt
    10921 gtgaaaaacc gacgaacatt cctgctggct ttccttgccg ttctgatcgc agcgctttac
    10981 ggaaccgcgg tctttgccgc cacccccaat aaatgcattc agtgccatac gaatgacgcg
    11041 ctgatgaagt ctctgcacaa gccgcccgtt ttgcccaaaa gcgatggcga ggggtgagcg
    11101 ggtgcccctc cgccagttcg gccggaagaa atgtacaaag gatatgtggt ggacaaggcc
    11161 ctgctggaca aagatcccca tttcaaggca ggctgcgtcc tttgccacgc gggaaacgac
    11221 ggggagctcg gcaaggaaaa agcccacctc ggaatgctga aaaagccctc ggacaatctg
    11281 cgttcctgcg gggtttgcca taaaaaggtt gccgccaact acgccaagtc gctgcactac
    11341 acgacgatcg gccagcggac gggcgtcatg tcgcggttct ccgccgcgga gttgaaaacg
    11401 tttgacgaaa aggtttttga aaaatcctgc cgaagctgcc atgcttcctg cggcgactgc
    11461 cacgtgaagg gcgcgccggt cggcgggatc agcatcggtc tcgtcgcaaa gcacaaattc
    11521 gttaaaaagg acgagggcaa aacctgcgcg ttttgccacg gcggccgagt ctatcccgaa
    11581 tacacggggg attacggcgg cgctccggat gttcattacc agaagggcat gctgtgcatg
    11641 gactgccaca agaaggaaga attccatggc gacggtacgg cctacaagtc caaaaatgaa
    11701 attccgcagc gcccggcctg caagagctgc cacccgaacg gcaaggaagc caaaatggaa
    11761 acgcaggttg cgcatcgtca gcatgagggc aaagtgtcct gctacggctg ccattccggg
    11821 tcggcttacc gcaactgcaa cgactgccac ggcggtcatg ccgcggccag ccccggcatg
    11881 attctgggtc gcagcccgcg ggacaaaaaa gtcctgacga cactgcgatt gattcccacc
    11941 gtccgcgata cgttcgctcc ggcgggcatc aaaatggagc atttcgacgc ggtgcccaac
    12001 tactgggata cgcccgcgca taacatcaaa aagcggacgg accggacccg caaatgcgac
    12061 gcctgccacg tggatcgcaa gaacttcctg accaaagaaa cgatgatcaa gaacggcgcc
    12121 aaagccaatg aagatttgat ttttgtgccg aaagccatta aaaattagga ggagcgcact
    12181 ctatttttag cccacaacaa aagaaaaaaa tgtagggcgt accttcaggt gcgccaaatg
    12241 aaccttgcaa aaaagcaggg cgggtctttt gacccgctct gctttttttc gttgctcatt
    12301 gcccgtcgct tgaaaatggg tctcgttttt aatattcaga gaagcttgat ttcaaaaaaa
    12361 tgaagttttc cccgcaggag ggcttccacg cgctcccggt cggctgcgtg aaagcgcagg
    12421 cagatgccgc aatcggaact gaggtggcga ggcacgtgga tcaatttgca gccgatgcct
    12481 gagcctttga cgattttttc cgcgcccagc gcgtggctta cggattcaaa aagaactacg
    12541 gcgaaatcgt ttcctgtcgt cacgagccgg gtttcccctt ccgcttgaca agttcccgca
    12601 gcgcctcgca ggcatcgtcc acctcctgct gcgtattgaa aaatccgaag ctgaagcgga
    12661 ccgttccccg aggaaaggtg ccgatcgtcc ggtgggccga cggcgcacag tgaagccccg
    12721 gccggcagag gattccccgc tgttcctcga aataatcggc ggcgtccgaa gacgtgacgt
    12781 ttttaatatt gaacgatacg acggcggttt gccgggatgc gtcctgcggg ccgtaaagaa
    12841 tcacttcatc ccgcaagtcc tgaagtccgg cgaggaaacg ggaggcaagc tcctcttccc
    12901 tggcgcgcac ggcctctacg gtttgttcca gaacaaagga aacacccgca cccaaaccgg
    12961 cgattccgat cgtgttgggc gtgccgctct cataccggtc cggcataaat tccggctgct
    13021 cctcaaattc ggagcggctg cccgttccgc ccatcatcag cggcgcgatt ctttgatcca
    13081 gcccctcgcg gatatagagc ccgcccgtgc cctgcgggcc gaacagagat ttatgcccgg
    13141 taaacgccag cagatcgatg ttcatttttt caacatcgat cggcaaagcg ccggccgtct
    13201 gggcggcatc gacgcaaaac atcacgtccg gtctgtcctg caggagctcg cctatggcct
    13261 caaccggcag aatcgtcccc gtcacattgg atgcgtgcgt caccacgact attcgggtgt
    13321 ttttccgcag gacttttttt agatcttccg ggtccatttc cccgcggggc gaacagggga
    13381 cgaccgacaa ttcgacgccg cgcccttcaa gaaaccgcag cggacgcatg acggaattat
    13441 gctccatgcc ggacgtgatt acgtgatcgc ccggccgaag ccctccctgc aaggcgatgt
    13501 tgagcgcttc cgtggagttt ttcgtcaaaa cgatgcgcag cgggtcttca acatggaaaa
    13561 gaagcgccag gctttcgcgc gcctcgcaaa taatgcggcc ggcgtcaatt gaaagcccgt
    13621 ggccggagcg tcccggattg gcgccgacgt tccgctgata ggccgtcatc gccgacagca
    13681 tcgcttccgg tttcggaaac gatgttgcgg cgttgtccag ataaatggtt ttcattaaac
    13741 tttccgtcag acggtgtttt gattacttgg ccgcgcggat gatgatgtcc gaaccatcct
    13801 gccgaatatc cgccttcaag ccgcgccggc tgagaagacg ggaaacgttt tcccgcgcga
    13861 cttcgttgtc caccagaatt tccaaatctt tattgccgtc tgcaatcgcc cggtcaacca
    13921 gaaccaccgg ctgcgggcag gacagccctc gagcgtcaac tttttttgta ctcatatctt
    13981 ttctccttcc tgaaaatcaa gcggatactt tatctcgctg cgtaaagccg acaaccagac
    14041 agaacaaaaa cccgatcagg acggcggccg gcccaaaggc ggcgatgcct ttgggggatg
    14101 aggcgatcgc aaagttatgg gcaattgccg ctcccacgat catgcccgtt gcaaaaattg
    14161 cagcgtccat gtcgccttcg ccggacagga agagctggcg tccggggcag ccgccggcca
    14221 gaacaaacga cagtcctgcc agcgtcatgc cgaggaagtt ccagaggtga tcggtgtggg
    14281 cgacgggctg gccttcaaaa ccaggcttga attgccccag aagcatattg gctataaacg
    14341 caaaaaccag aagcgccgcc acgccgctga tgagatgaaa gtcccgaatc agaattacat
    14401 cgcgaaacgc ccccatggtg caaaaccggc tgcgctgcgc caggccgccg atcacgagcc
    14461 cggcgatcag gctgatgagg aggggagcat gctgcgagcc cggccctttt gcgctgaaga
    14521 aaatcggacc gcccggcgca aaggaaacct gaaacaccaa cagcagcaac aaaccgatca
    14581 tcaaagccgg aaatacccag ccggccgctt tcttttgcgc atacgacctc cccaggctgt
    14641 agccgttttt cagaaaccca acgccgataa aaatgccggc aatcagcccc gcgagcccca
    14701 aaatggcgtt gccgtctccg ccggccagcc taaggagtgt tcgccaggga cagcccagaa
    14761 ataccagagc gccaatcatc gcgaagacgc cgagaaagaa acgaatcaga ggcgaagaac
    14821 cgccgcgagg cttatactca ccggcaagaa gggcggcgac aaaagcgccc agaacaaatc
    14881 ccatgatttc cggtcgcaga tattgaacga catcagcgcg atgcaatccc agggcgccgg
    14941 caatatcacg ctcaaaacag gcgacgcaaa tccccatatt gggcgggttc ccgaaatact
    15001 gcagaagggc cgcgccgatt cctattaccg ctcccactag aatgatgccc cagcgggttg
    15061 aaaaaatatt tttcatttcg atgcctcctt tataagatgc ggaatttcta gcagaagcgc
    15121 cgatcattat ccaattcata atgggaatat tatcatgatt gttataactg aatggaatgt
    15181 gctacaaaac aaagcaaaag aatgcgggtc cgctctttga tccagtaagg gtcgttttga
    15241 atttgtccac tcgctgcctg ccgcctgcgc catattgcaa aggcagcaac agaaaggcga
    15301 acgattgttt cggccgaaag cgtctttacc gattatggcg ttaatttggg ttaatcatat
    15361 ttcaataagc ttcggcgggc cgctcctgct ggacggggct tcgctgcaaa tcgaagccgg
    15421 ggagcgcatc ggccttctgg ggcgaaacgg ttcggggaaa tcaaccctga tgaaactgct
    15481 ctccggcgag attccgccgg atgacggtga aatttcccgc gataaaaata ttcatgtttc
    15541 cgtcatgcct caggaggtgc ccgatcttcg cggaaccgtc tatgacgttg tagccggtgg
    15601 ctgtcaggaa catcttgatt tacttcatga atatcatgac ttgaccctgc ggctgcattc
    15661 aggcggcgat catcagcttt tgaaaaaaat cgaacaggtc cagcaccggt tggaagcggt
    15721 cggcgcctgg cagtatcatc aaaaggtgga agaagtgatt tctcgggtaa gcctcgatga
    15781 aagttccgaa tttcttctgc tttcggccgg gttgaagcgc cgggtgcttc tggcgcgggc
    15841 gcttgttgcc gatcccgacg ttctgctgct ggatgaaccc accaaccatc tggatattga
    15901 cgccatcctc tggctggaag aatttttaaa aagttttgag aagacgctga tgttcgtcac
//
LOCUS       DAOPAF010000034        15765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_3794_length_15765_cov_58.066964, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000034 DAOPAF010000000
VERSION     DAOPAF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3794_length_15765_cov_58.066964"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PLQ77_04940"
     CDS             <1..1096
                     /locus_tag="PLQ77_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPL10054.1"
                     /translation="LCLYGGINFYFFFRARSVLHFSGPLQVLLLVLLILLILAPAVVR
                     TLESLHCEQAARFVACVGYIWMAFVFLFFVLSVSLELVRFLHKLMVPETGTLTLKAVT
                     FSLSVLLSAGLVVYGCVDARRIRVKNLQIPVQQVLPGNGKLRIVQISDVHVGIIIRND
                     RLTPILEKIREAKPDILVSTGDLLDGELDNIMMDAERFTAIPAPAGKFAVLGNHEYYA
                     GVSRSIEFTEAAGFQLLRDEVAQASGITIFGEDDITGRKPGSTQKSALFLKSLAEKQN
                     GFVLLLKHQPHIRETANFNLQLSGHTHGGQLFPFGFIVKLYFPRIYGLHTLAENKLLY
                     ISRGTGTWGPPVRIFAPPEITVIDLIGKKN"
     gene            1137..1949
                     /locus_tag="PLQ77_04945"
     CDS             1137..1949
                     /locus_tag="PLQ77_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPL10055.1"
                     /translation="MKKNYIHGYHPRENIRLQDQASTLAELLHSDTAYPAGSRVLEAG
                     CGVGAQTVALARSSPGASITAIDLSESSLAEAGRKAAAAGLTNVQFRQADIFHLPFAA
                     EAFDHVFVCFVLEHLTHPLEALGALQSVLRKGGTMTVIEGDHGSAYFHPASESAHRAI
                     QCQVELQRRAGGNALIGRELYPLLCRAGFSKVRVSPRMVYVDSSRRVLADGFTRKTFT
                     AMIEGVRQSALEAGLIEEETFDRGIRDLYRTAEDDGVFCYTFFKAVGEKPLK"
     gene            complement(2017..2280)
                     /gene="rpsT"
                     /locus_tag="PLQ77_04950"
     CDS             complement(2017..2280)
                     /gene="rpsT"
                     /locus_tag="PLQ77_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPL10056.1"
                     /translation="MATHKSAEKRDRQAKARRARNIAAKSAIKTKVKAVIAAVESKNR
                     ENSEEALKAAIPAVAKAASKGLIHKKNASRKISRLTKKVNALQ"
     gene            2587..3729
                     /gene="ald"
                     /locus_tag="PLQ77_04955"
     CDS             2587..3729
                     /gene="ald"
                     /locus_tag="PLQ77_04955"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPL10057.1"
                     /translation="MKIGTVRELKNHEYRVGLTPDNVMAYVKKGHQVFVQSGAGEMAS
                     FSDDEYRLCGAVIFPTAAEVWAKCDMMVKVKEPLEEEYPYLRPGLILFTYLHLAPNRP
                     LVDALFSSGVKAVAYETIEGRMGSLPCLRPMSEIAGRLSIQEGAKYLERPFGGRGILL
                     GGVPGVERGKVVIVGGGIAGTYAAKAAVGMGAQVTLLDISLTRLVYLDDIFGTSITTL
                     YSSETNVARSLAEADLVIGAVLIPGATTPRLIRQNHLKIMKQGAVIVDIAIDQGGCCE
                     TSRPTTHDDPVFILDGIVHYCVGNMPGAVPRTSTVALANTTLPYGMMIAETGLEKACE
                     FNEGLSKGVNIYEGVCANKNVAESLDLPYTPVHEVMQYPQICASAG"
     gene            complement(3850..5658)
                     /locus_tag="PLQ77_04960"
     CDS             complement(3850..5658)
                     /locus_tag="PLQ77_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL10058.1"
                     /translation="MAEKFISDRNLRFLLYEVNDVESLLKYSRFADHSREVFDMILDT
                     AMRMGKDLFKPLFEEMDRKAPEYVNGEVRVHPRVREIMRAAGDGGWIASTFPYDLGGQ
                     QLPFTIGVMLPTAIFGAANYSAAVYHGLTHGAAGLIASYGTQEMKDAYLPKMIAGEWQ
                     GTMALTEPQAGSSLTDLTTTATPTDQGYCKIKGQKLFISAGDHDGCDNVIHLALVRLK
                     DAPAGIKGISLFIIPKKRIKDDGSLEPNDVNCAGIYHKLGYRGSPITQLAFGENDDCR
                     GWLVGEANKGLAYMFQMMNGARIDVGLAAAVISSAAYYASLEYAKERPQGRKLTSKDP
                     LSPQIPIIEHADIRRMLLFQKAVVEGSLSLLFQCGIYEDLEHVTEGEERENYNLLLEL
                     LTPVAKTYPSEMGILSCSAGLQILGGYGFCEEFPLEQLYRDVRIHPIHEGTTGIQGMD
                     LLGRKMMMKKGKAAQLYLAEVRKAIKEARDDAELKPFADKLAEAVALLEKVTMHQFGV
                     AAKEGPEMFLADATVYLEFFGIIAIAWQWLRQATAAKKSLAKSPGEADENFYQGKLAA
                     FRYFFGYELPKIQGLSDRLLSSDGLTVKMKNEYFED"
     gene            complement(5796..7004)
                     /locus_tag="PLQ77_04965"
     CDS             complement(5796..7004)
                     /locus_tag="PLQ77_04965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL10059.1"
                     /translation="MSREVVFVDGMRTAFGTLGKTLSGILGEEMAGLCIKGLVARSKI
                     LEKGGVVDCCFMGSAIGPLTAINGARWPALASGLPESTSASYIEMQCGSAIDSINHAA
                     WKILAGQADVIIAGGYESYSSAPARFPMNTPPFRMIPPLPVAPSLAPSRDPATMNMGL
                     TAEALQKIYSIPRQAQDEFAYRSQMRTKKAIESGFCARDIVPITVPQGKKPPLEFKVD
                     EFPRLETTMEGLAKLPPAFTKEGTVTAGNASGRNDGAAFVLMMTREKAEELGCKPWAK
                     WLSGGDVGVDPKIMGIAPAYAVPQALKRAGIKMSQLDVMECNEAFAVQNLAVAAELEK
                     QTGEKVDWEKWNPNGGAIAYGHPNGASGSRIGIFCMRELMDKGGKYGCFSSCCGGGLG
                     VACIVENLTF"
     gene            complement(7264..7860)
                     /locus_tag="PLQ77_04970"
     CDS             complement(7264..7860)
                     /locus_tag="PLQ77_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10060.1"
                     /translation="MKKGWMVFALLMISLTVSGAATAQSRKPVLARTVNSMMDVNGDA
                     VVSVTEHAAFWQGRFKDIDIDRDGKLTAAEFEAANKEFFGTMDEDQNGSLVVKEYMAF
                     WCGPKAGVPARVKAVSKKKLDVNRDGKIGDDECVVFWAANFFDVDSNHDGKITTDEFM
                     AAMSRRFKEIDKNRNGWISLEEHTYFMSKNAGTAQKIK"
     gene            complement(7903..8769)
                     /locus_tag="PLQ77_04975"
     CDS             complement(7903..8769)
                     /locus_tag="PLQ77_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL10061.1"
                     /translation="MDLRERYIETNGIKLHVMESGPADGPMVLFLHGFPEFWYAWRKQ
                     IGYFADRGYLVVAPDQRGYNVSDKPAGVAAYNIDELAKDIIGLVDAYGRKQILLVGHD
                     WGASVSWWVALKYPDRVRKLAILNVPHPKVMARNLFANQRQMEKSWYIFYFQLPGAVE
                     KFGSAHNYAWPVHLLSTTSRPGAFTPEELEEYRRAFAQPGAFSAMVNWYRAMIQTQPE
                     PPQSFDVAMPMILLWGEDDVAMLSEMADQSMAYCKQGKLIKFPGVSHWIQHEEARKVN
                     ALIEELITIPKR"
     gene            complement(8889..10847)
                     /locus_tag="PLQ77_04980"
     CDS             complement(8889..10847)
                     /locus_tag="PLQ77_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL10062.1"
                     /translation="MKTLEHLLSPITIRSLTIPNRLVMPPMGTALGNPDNTVSEANLA
                     YIKRRAQSGAGLIITEITEVHPLGSASPRCIGVWDDKFIPGLSRLADAVHARGSKIAM
                     QLHHTGRENYLLQKKNKAVAPSAIPSYIFGFLGTPREMTLAEIEETIAAFGSAAVRAR
                     KAGFDAVELHGAHGYLLMQFLSAHSNQRKDQYGGDFRGRARFMIECLEEVRRQVGPDF
                     PMSIRISGEEYIKNGYTITDMEAILPDLVSAGADIINVSFGTHGSPAVNIDTPNPSAP
                     VEYAPGFKSHLARRVKEGAGVPVISVGRYTDPYFMDEVIARGDADMIAVARQHLADPD
                     FLKNAQAGHPEDTLECLACNQGCIERLSLEALPIRCAINPQTGQELMYPAGPAAVPRK
                     VWVVGGGPAGLTAAYEAARLGHQVTLFEQESQTGGNVRYAAKAPHKEVYGRYIQTLTA
                     KCKKKGVEIKTGAEVTEAMIEAGKPDAVILAIGAGKAACPAEGIDASVVCDAWQILDG
                     TVAPKEPVVVIGGGLVGMETADFLLEKGVRNITIVEMLPASPVLALAAHGYMLHKRLR
                     AAGIALMLGTRVKKIEENAVIVEVKGEEKRLEPVSQVIVAIGVTPRTALKDMLAKKSI
                     RHFVVGDASAPRRIIEATTEGAKAAWEV"
     gene            complement(10865..11668)
                     /locus_tag="PLQ77_04985"
     CDS             complement(10865..11668)
                     /locus_tag="PLQ77_04985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPL10063.1"
                     /translation="MFAMFDISQFSLKDKVAVVTGGSRGIGQAIAYGFAKAGAKVVIT
                     SRKAQDLEATACEMTAIGCEVLVVPAHLGKIGEVYRVVDTVLEKFGRIDILVNNAGAS
                     PAMASVLDTDERLWDTLMNLNLKGTYFMSQAAARVMKKQGGGKIINVASIDGFKPEPF
                     VSVYSISKAGVRMITKAFAMELARDNIQVNSICPGPISTKMMNSHWMHLPPEEAKKQK
                     EEMCKHLPTCRIGEPDEIAGAAVYLASEASNYTTGAEIVIDGGLLLGMH"
     gene            complement(11808..13310)
                     /locus_tag="PLQ77_04990"
     CDS             complement(11808..13310)
                     /locus_tag="PLQ77_04990"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPL10064.1"
                     /translation="MRTKQDYINGLSKMKRNIYFDGQLIDRTDELQMDCLNTIGTTYE
                     EAQKPENADLLTATSHLTGEKINRFTHIHQSTVDLHRKQDMTRMLCQKVGGCIQRCMG
                     IDGTNAIYNVSFEADKVKPGETNYHENFKKWLTRFQTGDLVGCCAQTDTKGDRLLRPA
                     DQPNPGAYVRIKERTKDGIIVEGCKLHISEASVADEVLVLPTRALRKEDKDYAVAFAV
                     PGDWDGLTQVVTIHNLRQREHYRRGFMPGATDSYMIFDNCFVPWERVFLAGEYQHGGV
                     LALLFALFHRHSYSGCKPAIGDVILGTAALAAECNNVHKESHVREKLAELIMTIELGY
                     AAGYTASDLGKPEVYIPGMGFVPFGPGSYIPHSIYCNVGRCLSGEAVWREAEILCDIA
                     GGVVATFPHEKDFTNPATGEALLKYTKRNPKMSPEDQAQFWRYLGDHLCSATGGIVNV
                     GNYHGGGSPIMEQIAITTQYDIESRKKLVKYIAGMSDGDREVFAPKPDKK"
     gene            complement(13382..14881)
                     /locus_tag="PLQ77_04995"
     CDS             complement(13382..14881)
                     /locus_tag="PLQ77_04995"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPL10065.1"
                     /translation="MRTKQDFINALSKMKRNVYYDGQLIDRTDELQSDCINVMGTTYD
                     EAAKPENETLMTATSHLTGEKINRFTHIHQSTVDLHRKQDMTRMLCQKVGGCIQRCMG
                     IDGTNAIYNVSFEADKVKPGETNYHENFKKWLTRFQKEDMIAACAQTDTKGDRMLRPA
                     EQPNPGAYVRIKERLKDGIVVEGCKVHISEASVSDEVLVIPTRALRPEDKDYAVGFAV
                     PADYDGMKQVVTIHNYRQREHFKRGFTPGLTDSYLIFEDCFVPWERVFLAGENLHGGI
                     LALLFALFHRHSYSGCKPAIGDVILGTAALAAECNNVHKESHVREKLAELIMTIELGY
                     AAGYTASDLGKPEVYIPGMGFVPFGPGSYIPHSIYCNVGRCLSGEAVWREAEILCDIA
                     GGVVATFPHEKDFTNPATGEALLKYTKRNPKMSAEDQAQFWRYLGDSMCSATGGIANV
                     GNYHGGGSPIMEQIAITTQYDINSRKKLVKYIAGMSGGDREALAPKVKK"
     gene            complement(14913..15650)
                     /locus_tag="PLQ77_05000"
     CDS             complement(14913..15650)
                     /locus_tag="PLQ77_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL10066.1"
                     /translation="MADHVRAKTRKNRIMDAALRIFAEKSFQEATISEISKEAGVSEA
                     TIYEYFGTKEDLLFAIPEKISNDTYEESEAVLPFIKDVEGRMRAIMLSYIQLYESNPH
                     YSALVLLQLMSNKRFRQTAAHAAIRRSAHRLLECIREGIANGTFRPDADAYLIRSILM
                     GTIEHLFIHWHMLGMPRREKTIMEMLDPFLEIVLNGIRAKKDQQGLTLHLNMEDARSL
                     LQTLQNDPALQSKAEGADASGKIRENN"
BASE COUNT         3096 a         4603 c         4452 g         3614 t
ORIGIN      
        1 tttatgcctt tacggcggaa ttaattttta tttctttttc cgcgccagaa gcgttcttca
       61 tttctccggc ccgctgcagg tcctgttgct ggtcctcctc attctcctta ttctggcacc
      121 ggccgtcgtc cggacgctgg aatcgcttca ctgcgagcag gccgcgcgct tcgtcgcctg
      181 cgtgggctat atctggatgg cgtttgtctt tctgtttttc gttctgagcg tttctctcga
      241 gctggtccgg ttccttcaca agctgatggt tcccgaaacc ggcactttga cgctgaaagc
      301 cgtcacgttc agcctgtctg ttttgctgtc cgccggtctg gtggtttacg gctgtgtgga
      361 cgcgaggcgc atccgcgtca agaacctgca aattcccgta cagcaggttc tccccggaaa
      421 tggaaagctc cgcatcgtcc agatctccga cgtccatgtc ggcatcatta tccgcaacga
      481 ccgcctcacg cccattctcg agaagatccg ggaagccaaa cccgacattc tggtttctac
      541 cggcgacctg ctcgacggcg aactggacaa catcatgatg gacgcggagc gctttaccgc
      601 aattccggcg cctgccggga agtttgctgt tctgggcaat catgaatatt atgcaggcgt
      661 ttcgcgctcc attgaattta ccgaagccgc cggctttcaa ctgttgcgtg acgaggtggc
      721 gcaggcgtct gggattacga ttttcggcga agacgacatc accggccgga agcccggctc
      781 tacgcaaaaa agcgcgctgt ttctgaaatc gctggccgaa aagcaaaacg gctttgttct
      841 gcttttgaag catcagccgc acattcggga aacggcaaat ttcaacttgc agctttccgg
      901 ccacacgcac ggcggccagc tttttccctt cggcttcatc gtcaaacttt atttcccaag
      961 aatatacggt ttgcataccc tggcggaaaa caagctgctg tatatcagcc ggggcacggg
     1021 cacctggggg ccgcccgttc gcattttcgc cccgccggaa atcaccgtga tcgatctgat
     1081 cggtaaaaag aactaattcc gtccaaaaaa ccgatttcaa agcaggcagg gtccggatga
     1141 agaaaaacta tatccacggt tatcacccgc gcgaaaatat ccggttacag gaccaggcct
     1201 ccacgctggc cgaactgctg cattccgaca cggcgtaccc cgcgggaagc cgcgttcttg
     1261 aagcgggctg cggcgtcggg gcgcaaaccg ttgctctggc ccgcagcagt ccgggcgcct
     1321 cgattaccgc catcgacctg tcggagagct cacttgccga ggccggccgg aaagccgcgg
     1381 cggcaggcct gacaaacgtt caattccggc aggccgatat ttttcatctg cccttcgcgg
     1441 cagaggcctt cgaccacgtt tttgtctgtt ttgtcctgga gcatctgaca catccgttgg
     1501 aggccctggg cgccctgcaa agcgtcctcc ggaaaggcgg cacgatgacg gttattgaag
     1561 gggatcacgg atccgcctat ttccacccgg caagcgaatc cgcccaccgg gccattcagt
     1621 gtcaggttga gcttcagcgc agggccgggg gcaacgccct gatcgggcgg gagctttatc
     1681 ccctgctgtg ccgggctggt ttcagcaagg ttcgcgtctc cccccgaatg gtgtacgtcg
     1741 attcaagcag acgcgtactg gccgacggtt tcaccaggaa aaccttcacc gccatgattg
     1801 aaggcgttcg ccaatccgct ctggaagcgg gcctgattga agaagaaacg ttcgatcggg
     1861 gcattcggga cctttacaga acggctgagg atgacggcgt gttttgttat acgttcttta
     1921 aggctgtcgg cgaaaagcca ttgaaataga gttcttttga aataaagcgg aaaaaaacac
     1981 ggcatcgccg tgggcgatgc catgtgcctg cgaaaattat tgaagagcgt ttactttttt
     2041 ggttaagcgg gaaattttcc gggaggcgtt tttcttgtga atcaagccct tggaagcggc
     2101 tttcgccacc gcgggaatcg ctgctttcaa tgcttcctcc gaattttccc tgtttttgct
     2161 ctccacagcc gcgatcaccg ctttaacctt ggtcttgatc gcggacttgg ctgcgatatt
     2221 gcgcgcgcgg cgggccttgg cctggcgatc gcgtttttct gctgatttgt gtgttgccaa
     2281 aaggttctcc tcccctgcat ccagttattt aaggtcaggc tgtttaaccc aattatttcg
     2341 ggctgtcaag cgcaatttct gccgtgtccc tttccgccgc cccggctccg cagcggggtt
     2401 gttcaatccg gacggacgat ccttgcgccc gtcaggtttg aatctgccgg atatccttga
     2461 cattcgaatc agcttttatt atatatcccg acccgcatga cgatccattc cgaaaagcgc
     2521 ggcccgtgcg gcgtgaacaa caaaatcgcg caatccaaat ccggaaaaac acaagaggat
     2581 ggctaaatga aaataggaac cgttcgagaa ttgaaaaacc acgaataccg ggtcggactg
     2641 acgccggaca acgtcatggc ttacgtcaag aaggggcacc aggtgtttgt gcagtcggga
     2701 gcaggagaga tggccagttt ttccgacgac gaataccgtt tgtgcggagc ggtgattttt
     2761 ccgacggccg cggaagtgtg ggccaagtgc gacatgatgg tgaaggtcaa ggagccgctc
     2821 gaagaggaat atccgtacct ccgtcccggt ctgatcctgt ttacctatct gcacctggcg
     2881 cccaaccgtc cgctggttga cgccctgttt tcgtcgggcg tgaaagcggt cgcctacgag
     2941 acgatagaag gcagaatggg aagccttccc tgcctgcgcc cgatgagcga aatcgccggc
     3001 cggctcagca tccaggaggg cgccaagtac ctggaacgtc cgttcggcgg ccggggcatt
     3061 ctgctgggcg gcgtgccggg cgtcgaacgc ggaaaagtcg tcattgtcgg cggaggcatt
     3121 gccgggacgt atgccgcgaa agcagccgtc ggcatgggcg cgcaggtgac gctgctggac
     3181 atcagtctga ccaggctggt gtatctcgat gatattttcg gcacatccat cacaacgctg
     3241 tacagctcgg agacgaacgt cgcccgaagc ctcgcggaag ccgacctggt catcggtgcg
     3301 gtcttgatcc cgggggcgac cacgcccagg ctgatccggc agaatcatct gaaaatcatg
     3361 aagcagggcg ccgtgattgt cgatatcgcc atcgaccagg gaggatgctg cgaaacgtcc
     3421 cgacccacca cgcatgacga tcccgtcttc atcctggacg gcatcgtgca ttactgcgtc
     3481 ggcaacatgc ccggcgcggt tcccagaacg tccaccgtag ctttggcgaa caccaccctt
     3541 ccctatggaa tgatgatcgc cgaaacgggg ctggaaaaag cgtgcgagtt caacgaaggt
     3601 ctttccaaag gcgtcaatat ttatgaggga gtctgcgcca acaaaaacgt ggcggaaagt
     3661 ctggacctgc cctacacgcc cgtccatgaa gtgatgcagt atccgcagat ctgcgcttcc
     3721 gccggataac ctcgcgccgc ggccggatac cccgggctga aacgatgcgt tttctctttt
     3781 gaaaccgctt caaaaaaatc aggcaggccc gactgcggcc tgcctgattc ctgaattgcg
     3841 gatggcctct taatcttcga aatactcgtt tttcatcttc acggtcaggc cgtcgctgct
     3901 aagcagccgg tcggacaagc cctgaatttt gggcagctcg tagccgaaga agtagcggaa
     3961 cgcggccagc ttgccctggt agaaattttc atcagcttcg cccggacttt tggccagcga
     4021 ctttttggcc gccgtggcct gacgcagcca ctgccaggcg atcgcaatga tgccgaagaa
     4081 ttccagatag accgtggcgt cggccagaaa catctccggg ccttccttgg cggccacgcc
     4141 gaactgatgc atcgttactt tttccaggag cgcgacggct tcggccagtt tgtcggcaaa
     4201 cggcttcaat tccgcgtcgt cccgggcctc cttgatggcc ttgcgcactt ccgccagata
     4261 caactgggcg gccttgcctt ttttcatcat catctttcgg ccgagcaggt ccatcccctg
     4321 aatgccggtt gtgccttcat gaatcgggtg gatgcgcaca tcgcggtaaa gctgttcaag
     4381 gggaaactcc tcgcagaaac cgtagccgcc cagaatctgc aggcccgcgg agcaggacag
     4441 gatgcccatt tccgagggat acgttttggc gaccggcgtc agcagctcca gaagcagatt
     4501 atagttttca cgctcctcgc cttcggtcac atgctccagg tcctcataaa tgccgcactg
     4561 gaacagcagc gacagcgacc cttccacgac ggctttctga aacaggagca tgcgccggat
     4621 atccgcgtgc tcaatgatcg gaatctgcgg cgacaacggg tccttggagg tcagcttccg
     4681 tccctgcggt ctttcttttg cgtattccag ggaagcgtag taagcggcgg acgaaatcac
     4741 ggccgcggcc agacccacgt caatgcgggc gccgttcatc atctggaaca tgtaggccag
     4801 acccttgttg gcttcgccga ccagccagcc gcggcagtcg tcgttttcac cgaaggcaag
     4861 ctgggtgatg ggggagcccc ggtagccgag cttgtgatag atgcccgcgc agttgacgtc
     4921 gttgggctcg agcgagccgt catccttgat gcgcttcttg ggaatgataa acagcgaaat
     4981 gcccttgatg ccggccggcg cgtccttgag ccggaccagc gccaggtgaa tcacgttgtc
     5041 gcatccgtca tggtcgccgg cggagatgaa cagcttctgg cccttgattt tgcaatagcc
     5101 ctgatccgtc ggggtcgccg tggtggtgag gtcggtgagc gacgagcccg cctgcggttc
     5161 ggtgagcgcc atcgtgccct gccattcgcc cgcgatcatt ttgggcagat acgcatcctt
     5221 catttcctga gtgccgtagg aagcgatcag gcccgccgcg ccgtgcgtca gaccgtggta
     5281 aacggcggcg gaatagttgg ccgcaccgaa aatcgccgtc ggcagcatca cgccgatggt
     5341 gaaaggaagc tgctggccgc ccagatcgta gggaaaggtg gaggcgatcc agccgccgtc
     5401 acccgccgcg cgcatgatct cgcggaccct gggatgcacc ctgacttcgc cgttgacata
     5461 ctcgggggcc ttgcggtcca tttcctcaaa caggggtttg aagaggtcct tgcccatccg
     5521 catcgcggtg tccagaatca tgtcgaagac ttcccggctg tgatcggcaa agcgggaata
     5581 tttcaaaagg gattccacgt cgttgacttc gtaaagaagg aatctcaagt tccggtcgct
     5641 gataaatttt tctgccatcc tgcactcctt tgtttgctgg aaaaacgcaa ggcccgtaaa
     5701 gagagggctt cgagattttg tgaaaaatct ccggagccat tcagccccgg agatgttgtt
     5761 gcacccgcaa gaggatcatc gcggaggaaa accgcttaga acgtcaggtt ttccacgatg
     5821 caggccacgc ccaggccgcc gccgcagcag gagctgaagc agccgtactt gcctcccttg
     5881 tccatcagtt cccgcatgca gaaaatgccg atgcgcgatc cggaagctcc gttgggatga
     5941 ccataggcaa tggcgccgcc gttgggattc catttttccc agtcaacttt ttcgccggtc
     6001 tgcttctcca gctccgcggc cactgccagg ttctgcacgg cgaaggcttc gttgcattcc
     6061 atgacatcca actggctcat cttgatgccg gcgcgcttca gcgcctgggg cacggcatag
     6121 gcgggggcga tccccatgat cttgggatcg acgccgacat cgccgccgga cagccatttg
     6181 gcccagggct tgcagcccag ttcttcggcc ttttccctgg tcatcatcag cacgaaagcc
     6241 gcgccgtcgt tgcggccgga ggcgttgccg gcggtgacgg ttccttcttt cgtaaacgcg
     6301 ggaggaagct tggccagacc ttccatcgtc gtctctaaac gcggaaattc gtccaccttg
     6361 aactccagcg gaggtttctt gccctgcgga accgtgatcg gaacgatgtc gcgcgcgcag
     6421 aagccggact ctatcgcttt tttggttctc atctggcttc ggtaggcgaa ttcatcctgc
     6481 gcctgacgcg gaatgctgta gattttctgg agcgcttccg cggtgaggcc catgttcatg
     6541 gtcgcggggt cgcgggaagg ggcgagcgac ggcgcgaccg gcaacggggg aatcatgcgg
     6601 aagggcggcg tgttcatcgg gaagcgggcc ggtgcgctgc tgtaagattc gtagccgccg
     6661 gcgatgatga cgtcggcctg gccggccaga atcttccagg cggcgtggtt gatcgaatcg
     6721 attgccgaac cgcactgcat ttcaatgtag gaagccgagg tgctctcggg caagccggac
     6781 gccagagcag gccagcgggc gccgttgatg gccgtgagcg gtccgatcgc ggaacccatg
     6841 aaacagcagt ccaccacgcc gcctttttcc agaatctttg atcttgccac aaggcctttg
     6901 atgcacaggc ccgccatttc ctcacccagg atgccgctca gggtctttcc cagcgtgcca
     6961 aacgccgttc tcatgccatc cacaaatacg acttctctac tcataacccc tcctctgtat
     7021 ttttgttttg taaacgatga aatcgtttgc tcgaatgttg ctcaagatcc gcgcagccca
     7081 acagcgcctc ttcttttgga gcggtatcat acatgaaaca tgacgtcaag aaatatacaa
     7141 cgactgttgt aaaaatcgcg atggaaaagc aagggcggaa agattccgcc cttgcatgaa
     7201 aaccggtttt ttacagcctg cggcaaaaac cttcattcat gccggaacat ataaacattt
     7261 gttttatttt attttttgtg cggttccggc atttttggac atgaaatagg tatgctcctc
     7321 aagggagatc catccgtttc ggtttttgtc aatctccttg aaccgcctgg acatggccgc
     7381 cataaattca tccgtggtaa ttttgccgtc gtggttcgaa tccacgtcga aaaaatttgc
     7441 cgcccagaaa acaacacatt catcgtcgcc gattttaccg tcccggttga catccagttt
     7501 tttctttgaa actgctttga ccctggcggg aaccccggct ttcggcccgc accagaaggc
     7561 catatattcc ttgaccacca gcgaaccgtt ctgatcctca tccatggttc cgaaaaattc
     7621 tttattggcc gcttcaaact cggccgccgt cagtttgccg tcgcggtcga tgtcgatgtc
     7681 tttaaaccgc ccctgccaga aagccgcgtg ctccgtcacg ctgacaaccg cgtcgccgtt
     7741 gacatccatc atggaattga ccgtccgggc caacacgggt tttcgggatt gagccgtcgc
     7801 cgcaccggaa accgtcaacg aaatcattaa caaagcgaaa accatccatc cttttttcat
     7861 aaaagcctcc tcaataaaat aatgaaacag cggggtcata ttctaacgct tcggaatagt
     7921 gatcaattcc tcaatcagcg cattaacctt tctggcttct tcatgctgaa tccagtgcga
     7981 gacaccggga aatttgatga gctttccctg tttgcagtag gccatgctct gatcggccat
     8041 ctccgaaagc atggcgacat cgtcttcgcc ccacagcaaa atcatgggca tcgccacgtc
     8101 aaagctttgc ggcggctcgg gctgcgtctg aatcatggcc cggtaccagt tgaccatcgc
     8161 ggaaaaagct cccggctgcg caaacgctct gcggtactct tccagttctt caggcgtaaa
     8221 ggcgccgggg cggctcgtgg tcgaaagcaa atgcactggc caggcataat tatgggccga
     8281 gccgaatttt tccaccgcgc cgggcagttg aaaataaaag atgtaccagc tcttttccat
     8341 ctgccgctga ttggcaaaga gatttctggc catgaccttg ggatggggaa cattcagaat
     8401 ggcgagcttt cggacgcggt cgggatattt cagcgccacc caccacgaca cggacgcccc
     8461 ccagtcgtgg ccgaccagca gaatctgttt ccgtccatag gcgtcgacga gtccaatgat
     8521 gtccttggcc agctcatcaa tattataggc cgcaacgcct gcaggcttgt cgcttacatt
     8581 atagccgcgc tgatcgggcg caaccaccag atagccccgg tctgcaaaat acccgatctg
     8641 tttgcgccag gcataccaga attccgggaa gccatggaga aaaagaacca tcgggccgtc
     8701 ggccggcccg gattccataa cgtgcagttt tattccgttg gtttcaatgt atcgctcacg
     8761 caaatccatt tcggcctcct tttattgagg ggatgagact gaagactatt ggatcatttt
     8821 gcggggcgct tcgcacccac tttttttatc ttttccttca gccttcggtc ttccgtcttc
     8881 agcctttttt atacttccca ggcggctttc gccccctccg tcgtcgcttc gatgatcctt
     8941 cgcggagcgg aggcgtcgcc cacgacaaaa tgacggatgc tttttttcgc cagcatgtcc
     9001 ttcagcgccg tgcggggcgt aacgccgatg gccacaatca cctgggaaac cggctccaat
     9061 ctcttttcct ctcccttgac ctcgacgatc accgcgtttt cttctatctt tttcacccgg
     9121 gttcccagca tcagggcaat cccggccgcc cgaaggcgct tgtgcagcat gtagccgtgc
     9181 gcggccagcg ccagaacggg ggacgcgggc agcatctcca cgatggtgat attccggacg
     9241 cccttttcca gcagaaaatc ggctgtttcc attcccacca gcccgccgcc gatgaccacc
     9301 accggctctt tcggcgcaac ggtcccgtcc agaatctgcc aggcgtcgca aaccacggac
     9361 gcatcgatgc cttcggccgg acaggcggct ttgccggcgc cgatcgccag aatgacggca
     9421 tcgggtttgc ccgcttcaat catcgcctcg gtcacttcgg cgccggtttt gatctccacg
     9481 cctttctttt tgcattttgc cgtgagggtc tgaatgtagc ggccgtaaac ctctttgtgg
     9541 ggcgctttgg cggcatagcg tacatttccg cccgtctggc tttcctgctc aaagagggtt
     9601 acctgatggc cgaggcgcgc ggcctcgtaa gcggcggtca gtccggccgg cccgccgccg
     9661 accacccaga ccttgcgggg aacagccgcc ggacccgccg gatacatcag ttcctgaccg
     9721 gtttgcggat tgatggcgca gcgaatcggc agcgcctcca gggagaggcg ctcaatgcag
     9781 ccctgattgc aggccaggca ctccagcgtg tcctcgggat gtccggcctg cgcgtttttg
     9841 aggaaatccg gatcggccag atgctggcgc gccacggcaa tcatgtccgc gtcgccgcgg
     9901 gcgatgactt cgtccatgaa ataaggatcc gtgtagcgcc cgacggaaat caccggaacg
     9961 cccgcgccct ctttcaccct gcgcgccagg tgggacttga agccgggggc atattcgacc
    10021 ggcgcgctcg ggttcggcgt atcgatgttc acggcgggac tgccgtgcgt gccgaaggaa
    10081 acattgatga tgtccgcgcc ggcgctcacc agatcgggga ggatggcttc catgtcggtg
    10141 atggtgtagc cgtttttgat atactcctcg ccggagatcc ggatcgacat ggggaaatcc
    10201 ggaccgacct ggcggcggac ttcctcgagg cattcgatca taaagcgcgc ccgtccgcgg
    10261 aaatcgcctc cgtactgatc cttgcgctga ttgctgtgcg ccgaaagaaa ctgcatgagg
    10321 agatagccat gcgctccgtg aagctcaacg gcgtcaaatc ccgcctttcg cgcccgcacg
    10381 gccgcgctgc cgaaagcggc aatggtttcc tcgatttcgg caagcgtcat ctcgcgcggc
    10441 gttccgagaa aaccgaagat ataactgggg attgccgaag gtgcgacagc cttgtttttc
    10501 ttctgcagca ggtagttctc gcggcccgta tggtgaagct gcatggcgat tttgcttccc
    10561 cgcgcatgca ccgcatcggc aagcctgctc aggccgggaa tgaatttgtc atcccagacg
    10621 ccgatgcatc gcggggaggc ggaacccagc gggtgcactt ccgtgatttc cgtgatgatg
    10681 aggccggcgc cgctctgcgc cctgcgtttg atataggcca ggttggcttc gctgacggtg
    10741 ttgtcgggat tgccgagggc tgttcccatg gggggcatga ccagccggtt gggaatcgtc
    10801 agcgaacgga tggtgatcgg ggacaacaaa tgctcgaggg tcttcataat cgttctcctt
    10861 cgcgttaatg catccctaac agcaatccgc cgtcgataac gatttcggcg cccgttgtgt
    10921 agtttgacgc ttccgaggcc aggtaaacgg cggctccggc gatttcatcc ggttccccga
    10981 tccggcaggt cggcagatgc ttgcacatct cttccttctg ctttttcgcc tcttccggcg
    11041 gcaggtgcat ccagtgggaa ttcatcatct ttgtgctgat ggggccggga cagatggaat
    11101 tgacctgaat gttgtccctt gccagttcca tggcgaaggc cttggtgatc atccgcacac
    11161 ccgccttgga aatggaatac acgctgacaa acggctccgg cttgaacccg tcgatggagg
    11221 cgacgttgat gatttttccg ccgccctgct ttttcatgac cctggccgcc gcctggctca
    11281 tgaagtaggt gcctttgagg ttgagattca tcagcgtatc ccagaggcgc tcgtccgtgt
    11341 ccaggacgga ggccatggcg gggctggcgc cggcgttgtt gaccagaatg tcgattcgtc
    11401 cgaatttctc cagaaccgtg tccaccaccc gataaacttc gccgattttg cccagatgcg
    11461 ccggcaccac cagcacctcg cagccgatgg ccgtcatttc gcaggcggtg gcttccagat
    11521 cctgcgcctt tcggctggta ataaccactt tggcccctgc tttggcgaaa ccgtaggcaa
    11581 tcgcctggcc gatgccgcgg ctgccgccgg tgacaaccgc cactttatcc tttaatgaaa
    11641 actgggaaat atcaaacatg gcaaacactc cttttttatg ttgttggatg cgtcagaaaa
    11701 gatgcagcgg aaattgcaac cccttttgat caggaaaaat acggggcgtc cgccgcgggg
    11761 aacggtgcga agccgctccc cgcaggcgac ccccgcgggt aagatggtta tttcttgtcc
    11821 ggtttgggcg caaaaacttc ccggtcgccg tcgctcatgc cggcgatgta tttcaccagc
    11881 ttcttgcggg actcgatatc gtactgcgtg gtgatggcaa tctgctccat gatcggagag
    11941 ccgccgccgt gatagttgcc gacgttgaca atgccgccgg tggccgaaca gagatgatcg
    12001 cccaggtagc gccagaactg cgcctggtcc tccggcgaca tcttcggatt gcgcttggtg
    12061 tacttcagga gcgcctcgcc cgtggccgga ttggtaaaat ctttctcatg cgggaacgtc
    12121 gccacgacgc cgcccgcgat gtcgcacaga atctccgcct cgcgccagac cgcctcgccc
    12181 gacaggcagc ggcccacgtt gcagtaaatg gaatggggaa tataggagcc ggggccaaac
    12241 ggcacaaaac ccattccggg aatatagact tccggcttgc ccagatcgga ggcggtgtag
    12301 ccggcggcat atcccagttc aatcgtcatg atcagttcgg ccagtttctc acggacatgg
    12361 gattccttgt ggacgttgtt gcattcggcg gccagcgccg cggtgcccaa aataacgtcg
    12421 ccgatggcgg gcttgcagcc ggagtaggaa tgacggtgga acagggcaaa cagcagcgcc
    12481 aaaacgccgc cgtgctgata ttcgccggcc aggaagaccc tttcccacgg cacgaagcag
    12541 ttgtcgaaaa tcatgtagga atcggtcgcg cccggcataa acccgcgtct gtaatgctcc
    12601 ctctgccgca gattgtgaat ggtcaccacc tgggtcaacc cgtcccagtc gcccggaacg
    12661 gcaaaggcca cggcgtagtc cttgtcttcc ttgcgcagcg ctcgggtggg cagcacgagg
    12721 acttcgtcgg ccacggaggc ttccgaaatg tgcagcttgc agccttccac gatgatgccg
    12781 tccttggttc gctccttgat gcggacgtac gccccggggt tgggctgatc ggcgggacgc
    12841 agcaggcggt cgcccttggt gtccgtctgg gcgcagcatc cgacaaggtc cccggtctgg
    12901 aagcgggtca gccatttctt gaaattctcg tggtaattcg tctcgcccgg ctttaccttg
    12961 tccgcttcaa acgatacgtt gtagatggcg ttggtgccgt caatgcccat gcaccgctga
    13021 atgcagccgc cgactttctg gcagagcatc cgggtcatgt cctgcttgcg gtgcaggtca
    13081 accgtgctct ggtgaatgtg cgtgaagcgg ttgatcttct ccccggtcag gtgagacgtg
    13141 gcggtaagca ggtccgcgtt ttccggcttt tgcgcttctt cataggttgt cccgatcgta
    13201 ttgaggcaat ccatctgcag ttcgtcggtt cggtcaatta actgcccgtc aaaataaatg
    13261 ttcctcttca ttttggaaag accgttaatg taatcctgct tggttctcat ggcgtcatcc
    13321 tttctgttga aatgatataa aaaggggcct cggccgtcag gccccttttg gtcagtccat
    13381 gttacttctt gactttcggc gccagggctt cacggtcgcc gccgctcatg ccggcgatgt
    13441 atttcaccag cttcttgcgg gaattgatgt cgtactgcgt cgtgatggca atctgctcca
    13501 tgatcggaga gccgccgccg tggtagttgc cgacgttggc gatgccgccc gtggccgaac
    13561 acatcgaatc gcccaggtag cgccagaact gcgcctgatc ctcggcggac atcttcggat
    13621 tgcgcttggt gtacttcagg agcgcctcgc ccgtggccgg attggtaaaa tctttctcat
    13681 gcgggaacgt cgccacgacg ccgcccgcga tgtcgcacag aatctccgcc tcgcgccaga
    13741 ccgcctcgcc cgacaggcag cggcccacgt tgcagtaaat ggaatgggga atataggagc
    13801 cggggccaaa cggcacaaaa cccattccgg gaatatagac ttccggcttg cccagatcgg
    13861 aggcggtgta gccggcggca tatcccagtt caatcgtcat gatcagttcg gccagtttct
    13921 cacggacatg ggattccttg tggacgttgt tgcattcggc ggccagcgcc gcggtgccca
    13981 aaataacgtc gccgatggcg ggcttgcagc cggagtagga atgacggtgg aacagggcaa
    14041 acagcagcgc cagtatcccg ccgtgcagat tctcgccggc caggaagact ctctcccagg
    14101 gcacgaaaca gtcttcaaaa atcaggtacg aatcggtcag ccccggcgtg aagccccgtt
    14161 tgaaatgttc gcgctggcgg tagttatgaa tggtcaccac ctgcttcatg ccgtcataat
    14221 ccgcgggcac ggcaaaaccc accgcgtagt ccttgtcttc gggacgaagc gcgcgggtgg
    14281 gaatcaccag cacttcatcg gagacggagg cttcggaaat gtgcaccttg cagccttcga
    14341 ccacgatgcc gtccttcagg cgttccttga tgcgcacata ggcgcccgga ttgggctgtt
    14401 cggccggccg cagcatgcgg tcgcccttgg tatccgtctg ggcgcaggcg gcaatcatat
    14461 cttccttctg gaagcgggtc agccatttct tgaaattctc gtggtaattc gtctcgcccg
    14521 gctttacctt gtccgcttca aacgatacgt tgtagatggc gttggtgccg tcaatgccca
    14581 tgcaccgctg aatgcagccg ccgactttct ggcagagcat ccgggtcatg tcctgcttgc
    14641 ggtgcaggtc aaccgtgctc tggtgaatgt gcgtgaagcg gttgatcttc tccccggtca
    14701 ggtgagacgt ggcggtcatc agcgtttcgt tctccggctt ggcggcctca tcatacgttg
    14761 tgcccataac gttgatgcag tcgctttgca gctcgtcggt ccggtcgatc agctgcccgt
    14821 cataatagac gttgcgtttc attttcgaca gggcgttgat aaaatcttgc ttggttctca
    14881 tagggggtac tcctttcttt gtgtatgctt ttttagttgt tttctctgat tttgccggaa
    14941 gcgtccgcac cctccgcctt cgattgcagc gccggatcgt tttgcagcgt ctgcagaaga
    15001 gagcgggcat cctccatgtt caaatgcagc gtaagcccct gctgatcctt tttcgcccgg
    15061 atgccgttca aaacgatttc cagaaacgga tccagcatct ccataatggt tttttcgcgc
    15121 cggggcattc ccagcatgtg ccagtggata aacagatgct cgatggtgcc catcaggatc
    15181 gagcggatca ggtaggcgtc ggcgtcggga cggaacgtgc cgttggcgat cccttcgcga
    15241 atgcattcga gcaggcggtg ggcggaacgg cgaatggccg catgcgcggc ggtctgccgg
    15301 aagcgcttgt tggacatcaa ctgcagcagc accagcgcgg agtaatgcgg attgctctcg
    15361 tagagctgaa tgtagctgag catgatggcg cgcattcggc cttccacatc tttgataaag
    15421 ggcaggacgg cttcggattc ttcatacgta tcgttggaga ttttttcggg aatggcaaag
    15481 agcagatctt ccttggtgcc gaaatattca taaatcgtcg cctcggaaac accggcttcc
    15541 ttgctgattt cggatatcgt tgcctcctga aaactttttt cggcaaaaat ccgcaaagcc
    15601 gcatccatga tgcggttctt cctggtcttc gcacgaacgt gatcggccat tgcaatatcc
    15661 ttttttaagt ttgatttaac ctacatcttc atagtcccac tgtcaagtat tattttttat
    15721 catagtgcga ctattttttt taaacgatat tcatagtcaa tggtc
//
LOCUS       DAOPAF010000035        14741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4107_length_14741_cov_40.611603, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000035 DAOPAF010000000
VERSION     DAOPAF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4107_length_14741_cov_40.611603"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..574
                     /locus_tag="PLQ77_05005"
     CDS             32..574
                     /locus_tag="PLQ77_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPL10067.1"
                     /translation="MRLKTKDGGWNPYFAGALVGVLAIVSVCATTELMGKTNYLGAST
                     TFVRAAGFVEQVFASEHVAQNAYFAATKIKVDWQFMLIIGIFLGSLISSLTDKSFLVE
                     KVPPIWKERFGSSMAKRGAGAFLGGIVAMIGARLADGCPSGHGLSGMMQLSASSFVAL
                     VLFFAAGALTAVLVYKRRAS"
     gene            571..1101
                     /locus_tag="PLQ77_05010"
     CDS             571..1101
                     /locus_tag="PLQ77_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPL10068.1"
                     /translation="MSIDQILGLVTGVVFGFLMQKGRVIRFEKQVGALLIKDMTIFKF
                     MLSSILVGMVGIFILSDMGIITFSHKPMNVGAVVIGAILFGIGWAVMGFCPGTSVAAV
                     GEGRVHALFGIAGMLVGAAVFAEIYPFLQSTVMAWQDFGKISLPGALGISHWVLVPIF
                     WAATIGLFVVFEKNGL"
     gene            1257..1691
                     /locus_tag="PLQ77_05015"
     CDS             1257..1691
                     /locus_tag="PLQ77_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPL10069.1"
                     /translation="MRTNTKEDLIGQLKENGLKITPQRLAIIDALVENRYEHPGATLI
                     FNEARKKAGRVSLSTVYATLKEFSESGLIRQMEFDRLENRYDVDLSDHVNLICKRCGK
                     VVDYHIPAPLEPKNIVRKSGFVVTDTRIEFHGYCRDCLKRPD"
     gene            1813..4023
                     /gene="katG"
                     /locus_tag="PLQ77_05020"
     CDS             1813..4023
                     /gene="katG"
                     /locus_tag="PLQ77_05020"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPL10070.1"
                     /translation="MAEESKCPVTGAMKKSISGRGASNRDWWPNQLNLKILHQHTQKS
                     NPMGEAFDYAKEFKKLDLKAVKKDLYALMTESQDWWPADWGHYGGLFIRMAWHSAGTY
                     RMGDGRGGAGSGSQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNC
                     ALESMGFKTFGFAGGRVDVWEPEEDIYWGSEEEWLATSDKPKSRYTGERDLENPLASV
                     QMGLIYVNPEGPDGNPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGPA
                     THVGPEPEAAPIEEQGLGWKSSFKSGKGIDTISSGIEGAWKPNPTNWDMGYLKVLFKY
                     EWELVKSPAGANQWLAKNVEEEDMVVDAHDPSRKRRPMMTTADLSLRYDPIYEPIARR
                     YSANPKQFADAFARAWFKLTHRDMGPRSRYLGPEVPKEVLIWQDPIPAVNHKLISKSD
                     AAFLKSKILASGLSVSGLVSTAWASASTFRGSDMRGGANGARIRLAPQKDWEVNEPER
                     LARVLKMLERIQKAFNKEQGGDKKVSLADLIVLAGCAGVEQAAKNAGYKINVPFSPGR
                     MDASQKQTDAASFGVLEPKADGFRNYQKARFTVLPEELLVDKAQLLTLTAPEMTVLIG
                     GLRVLGANFSGLPHGVFTSRPGALTCDFFVNLFDMGTEWKASVNDPGVFEGYDRKTGE
                     LKWTGTRVDLIFGSNSQLRAVAEVYACQDSPEKFLGDFVAAWNKVMNLDRFDLA"
     gene            4057..4554
                     /locus_tag="PLQ77_05025"
     CDS             4057..4554
                     /locus_tag="PLQ77_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPL10071.1"
                     /translation="MSKTEDALKEAFAGESQANRKYLAFAAKADQEGFAQAARLFRAA
                     AEAETIHAHAHLKALKGIRSTKENLQEAVDGETHEFKSMYPAMIEAAKAEGHKEAERS
                     FIFANEVEKIHAGLYQQMLDNLDGAKETYGYYICPVCGYTAEKEAPETCPVCGAKGKM
                     FKKVD"
     gene            4579..4953
                     /locus_tag="PLQ77_05030"
     CDS             4579..4953
                     /locus_tag="PLQ77_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPL10072.1"
                     /translation="MAKRNEVFKCEVCGHIVEVLHEGKGELVCCNQPMKLLTENTTDA
                     AKEKHVPAIEKVAGGFKVKVGSVAHPMEEKHYIEWIEVIADGRAYRQYLAPGQAPEAF
                     FPIEAATITAREYCNLHGLWKA"
     gene            4990..5508
                     /locus_tag="PLQ77_05035"
     CDS             4990..5508
                     /locus_tag="PLQ77_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL10073.1"
                     /translation="MAKLYRCTICGDAYIGASPPANCPFCGAHMGYMAEAKEAVVNFD
                     VPLSVKDQAKAEHALKVEISNSAFYMCAANQTDDPEGKILFKALGKVEAEHASIWRKI
                     LKLGSVPPGSDACHTKNVANLKESHEREIRAIEFYRKSAAEADHPRIRQLFEALVEIE
                     TDHLHLSEERLK"
     gene            5745..8741
                     /locus_tag="PLQ77_05040"
     CDS             5745..8741
                     /locus_tag="PLQ77_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPL10074.1"
                     /translation="MIPDVRNSINPQKSLTALLMKMLLGIVAAGFQLRRSYHQYLKTS
                     EGWIDLTVGLRTESRSVECAIRFKDGRVSVLGRIPEKTDVTIVFRSDAVVKKLLAATP
                     SDQIFMLLKSEMRVEGSMMYLNLFFFLLSVLMNKKQIRQLQKEREASREAEIKKSPDS
                     RKELSDELQARRATRLKGEKVDGGVHYLEDPYLPEFGLDDFPRLKKFLDVHLTAKPEI
                     CPEMPRLLTEWHKKNGFETDAKGRPWEPATRKALSYKYLMENRAPVIAGDCLLAGTTT
                     SREVGCIVYPEGSGGLIWNELFTIAHRSYNPFEISEETRNLLHYEVFPYWAHRNFREW
                     VRDRYNNPLCMQIDERYAYYFNWKQATISHTIPDFPKMLRLGTSGIIGEIEQELKNVQ
                     GNAEKKATLEAMILTLEGLTAYSKNLAAQAKKDAAVQADPVRKKELERIAAACSQVVE
                     KPARSLHEAVQAMWITWVGLHMESMNAGLSLGRLDQWLQPYFLADMQQLSSREDRAEY
                     IRDAIELIGHFYMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPEGLDAVNDMTYI
                     FLKVTEMLSLRDPNVNARFTPGISSDAYLKRLCEVNLITAATPSMHNDKAMFAALSPM
                     GYDIRDIRNWSATGCVEPTLSGKHMAHTNMQMMNMVAALEMALNNGLHPLTDWKLGPE
                     TGAIEQGDFNSFEDFFNAFTEQFAFLIDRSIEYNDLLALAHQYLRPTPLLSSVIDGCI
                     TKGKDVTRGGATYNSSGTAIVGLADVTDSLMTIKKLVFDEKKISFAHLKKAVDGNFEN
                     DPELLAMVRRKVPLFGSGNAEAVEMANRLTRWSHDHYAGLTHYRGGKYTTGFWTMSQH
                     VAFGILTGALPSGRQAGKPFTPGLTPQPFASKNLLDNIRDVAKLDPVNLNNNIAFNVK
                     VVPSAADTREKTVHNMFSYVKTYFDLGGMQMQMNVVTSAMLRDAMAHPENYRNLIVRI
                     SGYNAYFVTLNKDMQRELIERAEYAM"
     gene            8752..9693
                     /locus_tag="PLQ77_05045"
     CDS             8752..9693
                     /locus_tag="PLQ77_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPL10075.1"
                     /translation="MAQTEESQARKPLILEIKGNSLDDGPGIRTVVFFKGCPLSCSWC
                     HNPESKLARVEIGYDRKECIGCGTCTAVCPEKAVSPQNIHPVDREKCTLCFRCVEECP
                     AGALARVGRSLSIDEIAAEVLKDKPFFDTSGGGATFSGGEPVMFMEFLSELLHALKAY
                     QVHTLLETSGFFDFEKFERLLLPCLDMIYYDIKIMDEKAHRIHTGVSNQRILENFERL
                     FKISRDTGITLLPRTPLVPGITDRHENIQGIARFLAELGVRNAALMPYNPLWHEKADK
                     IGADNPHRGHAVMAAWMEKEKVEACRRIFSEEGIQVV"
     gene            10005..10358
                     /locus_tag="PLQ77_05050"
     CDS             10005..10358
                     /locus_tag="PLQ77_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPL10076.1"
                     /translation="MEIKFKIWLEKDGHALFGEGRDQLLKAIDECHGLYGAAKKLKMS
                     YRAAWGKIKLTEERIGKKIVETGEDKKLHLTKDAKKILDEFEKLEKDVDGFLHARRST
                     FIVLKDPPTKSDEKK"
     gene            10627..13203
                     /locus_tag="PLQ77_05055"
     CDS             10627..13203
                     /locus_tag="PLQ77_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPL10077.1"
                     /translation="MEEKFSMKRRSFLKLAGAAGVATAMTAFPFRNMRAAWAFGDHPQ
                     EQLPYRITKRVPQVCARACEADCAYNVVVGVDPATGLERALTLEGRPEDPVSNGKFCI
                     KGMGFVDSMYDPDRLMVSLKRTNKTKGTDVDPGWIIMKTEDAVNEIIARMKTYKPEEI
                     LMASPGDPYTNRLCQSIGCTRSDQRTECFGTHYYINCLTLTNPPNKFYSSCYTPSHHV
                     CGYDYDTCKYQVWFGFDSFSKCGKAGMLNHWAAGKRNGGKIVMFNPVRTPMTDNFASE
                     YYAIKPGTDLAVALAMINQIMAGKKYNKEFMTKYSDGAALVDVEKQTHLKTADGMFLA
                     WSVKNKKAEPIDECDDPALDGSYTVTLDGRQIKAKTVLQLLAEATKTYTPQWAAKISD
                     VPAKAISKIARDFAAAAPYALIPTYKRDAAGPNYANSWRLRHAIQILNSLAGSIDHEG
                     GMLLLHDVKIPWIDEISPPALPYPEQPSQPVDFRNEFPVTDDIYRKKDFSAPGHYGMV
                     GWGLYKTNRAKVAFFRNPHRGLFAMIHSQMFEAASQKLELVVDWNLYLDDLGYFCDYV
                     VPAPHQFEEGKMDLRLYYPKYPCIVGGSPVQKSPGDQIGWGGFAMKIGLAMAPKYWTT
                     DGSGNPAKKIPTNMGDVAVKKAGIADSAADFMKKGGLWIDKKEYQNYKQIEQIGYGKP
                     RGRVKLYINEFAEVGHSPLPVWHARWHETTGDYKFSLLITRAPWYMHADPNFINNPVL
                     KEVTVRNHMDTAWINPEAAAKLGLKEGDGVIIENDPTYMKDLPRPQKAKVHLTKRITR
                     NDCVLLFHGIGHRAKNLKVAANFGYRDGDLIPQKDPAMLKKFDPTGMGWVEDVFVSIK
                     KM"
     gene            13216..13920
                     /locus_tag="PLQ77_05060"
     CDS             13216..13920
                     /locus_tag="PLQ77_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL10078.1"
                     /translation="MKRYAILLDDTFCTGCNSCAYRCIQEFRYHDQASKGLFRTFVQI
                     NDDGLYQKRCMHCLEPDCVENCPVKALTKSEYGPVLYDAKKCIGCQTCVRVCKFHVPQ
                     FDADTKKIVKCSMCAHRINDDKKPACVEICPTGALQFGEYEAMKKLATKTAKEKKLKL
                     YGYEENGGTHLFVLAKEDPVKFGYPKVAKKALKGKAALDGELGVPALAALAVVGFKKY
                     SERRTRLETRTEKEKK"
     gene            14025..14705
                     /locus_tag="PLQ77_05065"
     CDS             14025..14705
                     /locus_tag="PLQ77_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HPL10079.1"
                     /translation="MNNQTTKTRRTGPDDRLIELQAKAALFNLLAQAFAYPDQLLMEK
                     LVSGEFAAILDESLGVLQPEGAWADRIAVVKRQYTAAGQNAADLLLELEKDYTRMFFS
                     SKPRLVYLFESVYKEGKLLQESTFEIARLYYEAGLNLVDDFKLPPDHIAVEFEFMAYL
                     YFNELAAVKSENRENEALAQKLQKEALEKHLTNFGRTLAEKVVTHAATDFYKIMAAVA
                     ASVLAAGR"
BASE COUNT         3662 a         3921 c         4137 g         3021 t
ORIGIN      
        1 tggctaattc aatcaacgaa aggaggcaac gatgagactt aaaacaaaag acggaggatg
       61 gaatccttac tttgccggag cgctggtggg cgtcctggcg attgtttcag tttgcgccac
      121 gacggagctg atgggcaaaa ccaattatct tggcgcgtcc accacttttg tccgcgccgc
      181 cggttttgtg gagcaggttt tcgcctcgga gcatgttgct caaaacgctt attttgccgc
      241 cacaaaaatc aaagtggact ggcagttcat gctcatcatc ggaatcttcc tgggctcgct
      301 gatctcttct ctcacggata aaagttttct ggtggaaaaa gtgccgccca tctggaagga
      361 acgcttcggc tcctccatgg ccaaaagagg cgcaggcgct ttcctgggcg gcatcgtggc
      421 catgatcggt gcgcggctgg cggatggctg tcccagcgga cacggactca gcggcatgat
      481 gcagttgtcg gcaagctctt ttgtggcgct ggtgctgttt tttgcggccg gcgctctgac
      541 cgcggtgctg gtatacaaaa ggagggcatc atgagcatcg atcaaatttt gggtttggtt
      601 accggtgtgg tgttcggatt tctgatgcag aaagggcggg tcatacgttt tgaaaaacag
      661 gttggcgcgc ttttgataaa agacatgaca attttcaaat tcatgctttc ctcgattctg
      721 gtcggcatgg tgggaatctt cattctctcg gatatgggaa tcattacttt cagccacaaa
      781 ccgatgaatg tgggcgctgt ggttatcggt gcaattcttt tcggcatcgg ctgggcggtg
      841 atgggattct gccccggaac ttccgtcgcg gctgtggggg aaggccgtgt gcacgctttg
      901 ttcggcattg cgggaatgct cgtcggtgcg gcggtatttg ccgagattta tcctttcctt
      961 caatcaaccg tcatggcgtg gcaggacttc ggcaaaatca gcctgccggg cgctctgggt
     1021 atttcccact gggtgcttgt gccgatcttc tgggcggcaa cgatcggcct gtttgtggtg
     1081 ttcgagaaaa atggtctgta aaatctgaac gttatcaggt tcctgtccac tggcatcgaa
     1141 ggctggtatg ccggtggacg ggaatcaatt gatcgcccgt tcttccaatt tgcaaaaacc
     1201 gcttgacagg acgcgccgga tcagggtagg attttccaaa ctggaatgat tccgatatga
     1261 gaacgaatac caaagaagat ctgatcggtc agttaaaaga aaacgggctc aaaattacgc
     1321 cgcagcgcct ggcgattatc gatgcgctgg tggagaaccg ctacgagcat cccggcgcga
     1381 cgctcatctt caacgaagcc cggaaaaaag ccgggcgcgt cagcctctcg acggtgtatg
     1441 cgacgctcaa ggaattttcc gaaagcgggc tgatccgcca aatggaattt gaccggctgg
     1501 agaaccgata tgacgtggat ctctccgatc atgtgaatct catctgcaaa cggtgcggaa
     1561 aggtggtgga ctaccacatt cccgcgccgc tcgagccaaa aaatattgtc cgaaaatcgg
     1621 gatttgtcgt taccgacacc cggatcgagt ttcacggcta ttgccgggat tgtttgaagc
     1681 gccccgactg attttttgat tctgcggttc cgcacgaggc aacaccgatg gcatgcgaag
     1741 cgaggggaag ggtcttaccg tttcctactt agaatgattc caatttaacc agtgaggaaa
     1801 ggaggaaatg aaatggccga agaaagcaaa tgtccggtga cgggcgcgat gaaaaaatcc
     1861 atttccggca gaggcgcctc aaaccgggac tggtggccga atcaattgaa tctcaaaatt
     1921 cttcatcagc atacacagaa aagcaatccg atgggcgaag cgtttgacta cgccaaagaa
     1981 tttaaaaaac tggacctgaa agccgtcaag aaagacctct acgcactgat gaccgaatcc
     2041 caggactggt ggcccgccga ctggggccat tacggcggac ttttcattcg aatggcctgg
     2101 cacagcgcag gcacctaccg catgggcgac ggccggggcg gcgcgggctc cgggtctcag
     2161 cgtctggcgc cgcttaactc ctggcccgac aacgtaaacc tcgataaggc tagaaggctt
     2221 ttgtggccga tcaagcaaaa atacggcaaa aaaatctcct gggccgatct gatgattctg
     2281 gccggcaact gcgccctgga gtcgatgggc tttaaaacct tcggattcgc cggcggacgc
     2341 gtggacgtct gggagccgga agaagacatc tactggggca gcgaagagga atggctggcc
     2401 accagcgaca agcccaagag ccgctacacg ggagagcgcg atctcgaaaa tcctctcgcc
     2461 tccgtgcaga tggggctgat ctacgtgaac cctgaaggtc cggacggcaa tcccgatccg
     2521 gtcgcctccg gccgcgacgt gcgcgaaacc tttgcgcgta tggccatgaa cgacgaagaa
     2581 accgtcgcgc tggtcgccgg cggacacact ttcggcaaat gccacggcgc agggccagca
     2641 acccacgtgg gacctgaacc ggaagcggct cccatcgaag agcagggtct tggctggaaa
     2701 agcagcttca aaagcggcaa aggcatcgac acgatttcca gcggcatcga aggggcctgg
     2761 aaaccgaatc cgacgaactg ggatatgggc tacctgaagg tgctcttcaa atatgagtgg
     2821 gaactggtca aaagcccggc cggcgccaat cagtggctgg ccaagaatgt ggaagaagaa
     2881 gacatggtgg tggacgcaca cgatccgtcc aggaagcgtc gcccgatgat gaccacggcg
     2941 gacctctcgc tgcgctacga cccgatctat gaaccgattg ccaggcgtta ttcggccaac
     3001 cccaaacaat ttgccgacgc cttcgcccgc gcctggttca agctgacgca ccgcgacatg
     3061 ggcccgcgtt cgcgttatct cggcccggaa gtgcccaaag aagtgctaat ctggcaggat
     3121 cccattcccg ccgtcaatca caagctgatc agcaaatcgg acgccgcctt cctcaagagc
     3181 aaaatcctgg cttcgggcct gtccgtttcg ggactggttt cgacggcctg ggcgtccgct
     3241 tccacgttcc gcggctccga tatgcgcggc ggcgccaacg gcgcgcgcat tcgcctggcg
     3301 ccgcaaaagg attgggaagt gaacgaaccg gagcgtctgg ccagagtgct caaaatgctc
     3361 gagcgcattc agaaagcgtt caacaaagag caggggggcg acaagaaggt atcgctggcc
     3421 gatctgattg ttttggccgg ttgcgccggt gttgaacagg cggcgaaaaa tgccgggtac
     3481 aaaataaatg ttcccttctc gcccggccgc atggacgcat cgcaaaaaca gaccgatgcg
     3541 gcttcgtttg gcgtgctgga accgaaagcc gacggtttcc gcaactatca gaaggccagg
     3601 ttcaccgtgc tgcccgaaga gctgctggtg gacaaggcgc aactgctcac gcttaccgcg
     3661 ccggagatga cggtgctcat cggcggcctg cgcgtgctgg gcgcaaactt cagcggcctg
     3721 ccgcacggcg ttttcacctc gcgtccgggt gcgctcacct gcgacttctt cgtcaacctg
     3781 ttcgacatgg gcaccgagtg gaaggcatcc gtgaacgatc cgggtgtgtt tgaagggtac
     3841 gaccgcaaga ccggcgagct caaatggacc ggcacccgcg tcgatctgat tttcgggtcc
     3901 aactcgcagc ttcgggcggt ggcggaagtt tacgcctgtc aggactcccc ggagaagttt
     3961 ctgggcgatt ttgtggcggc ctggaacaaa gtcatgaatc tggaccgttt cgacctggcg
     4021 tgatattaac aataaaatat aatgaaggag aaaatcatgt cgaaaacaga agatgcgtta
     4081 aaggaagctt ttgcaggaga atcacaggcg aaccgaaaat atctggcgtt tgcagccaaa
     4141 gccgaccagg agggatttgc ccaggccgca agactattcc gggcagccgc ggaggcggaa
     4201 accattcacg cccacgccca tttgaaggcg ttaaagggca tccgctccac aaaggaaaac
     4261 cttcaggagg cggtcgatgg agaaacgcat gagttcaaaa gcatgtatcc tgccatgatc
     4321 gaagcggcaa aggcggaagg ccacaaggag gcggagcgct catttatttt cgccaacgag
     4381 gtggaaaaga ttcatgccgg actttatcag caaatgctgg acaatctgga cggggcaaaa
     4441 gaaacctacg gctattatat ctgtccggtc tgcggctata cggcggaaaa agaagcgccg
     4501 gaaacctgtc cggtgtgcgg cgccaaaggc aagatgttta aaaaggtcga ttaatcattc
     4561 taaagaagga ggtttgtcat ggcaaaaaga aacgaagtct ttaaatgtga ggtatgcggc
     4621 catatcgtgg aagtgcttca cgaaggcaag ggtgagctcg tctgctgcaa tcagcccatg
     4681 aagcttttga cggaaaatac cacggatgcg gcgaaagaaa agcatgttcc ggcgattgag
     4741 aaagtggcgg gcggcttcaa ggtaaaagtg ggcagcgtgg cccacccgat ggaagaaaag
     4801 cattacatcg aatggatcga agtcatcgcg gacggcaggg cctatcgcca gtatctggcg
     4861 cccggacagg cgccggaagc gtttttcccc atcgaagccg ccacgattac agcccgggaa
     4921 tactgtaatc tgcacggtct gtggaaagca taagagaaag caggcaaatt tttgccgaaa
     4981 ggaaaagaca tggccaagtt ataccgatgc acgatatgcg gcgatgcgta tatcggcgcc
     5041 agtcctccgg cgaactgccc gttctgcggc gcgcacatgg ggtatatggc cgaggcaaaa
     5101 gaagcagttg taaattttga tgtgcctctg agcgtgaaag atcaggccaa agccgaacac
     5161 gcgctcaagg tggaaatcag caattccgct ttctacatgt gcgccgcgaa tcaaaccgat
     5221 gatcccgagg gaaagattct gttcaaggcg ctgggcaagg ttgaggcgga gcacgcgtcc
     5281 atctggagaa aaatcctcaa gctgggaagc gttcccccgg gaagcgacgc ctgccacacg
     5341 aagaatgtcg caaacctgaa agaatcccac gagcgggaaa tccgggccat tgaattctac
     5401 cggaagtcgg ctgccgaagc ggatcatccg cggattcggc agctttttga agcgttggtg
     5461 gaaatcgaaa cggaccacct gcacttgtcg gaagaaaggc tgaagtaaat gaatcccgtg
     5521 gaaccggggg acactatccc ccggtttcgt gccatgaaat ctttccatca gccacaggaa
     5581 gagcttaaat attctccgcg gtttcgcgaa cacctctcac tatccaactc ttcttgacaa
     5641 gtcccgtcct gtgtgtcatc cattgcaacc ggagagcgaa actcaagagg ttgactgaaa
     5701 aaactgattc ttctggaaac cagatttcac agggagaccc tgtcatgatt cctgatgtcc
     5761 gcaacagcat aaatccccaa aaatccctga cggcgctgct catgaaaatg ctgctcggga
     5821 ttgtggcggc cggttttcaa ctccgccggt cttatcatca gtacctgaaa acctcggagg
     5881 ggtggattga tctgaccgtg ggcttgagaa ccgaatcccg ctccgtggaa tgcgccatcc
     5941 gttttaagga cggccgggtg tccgtgctgg gccgtatccc ggaaaagacg gatgtaacga
     6001 ttgttttcag atccgacgcc gtggtgaaga aacttctcgc ggccacgccc tccgaccaga
     6061 ttttcatgct gctgaaaagt gaaatgcggg tcgaaggaag catgatgtac ctgaacctgt
     6121 tttttttcct cctgtccgtt ctgatgaaca aaaagcaaat ccggcagctc cagaaggaaa
     6181 gagaagcttc cagggaagcc gagatcaaaa aatcacccga ttcccggaag gaattaagcg
     6241 atgagctcca ggcaaggcgc gccacaaggc tcaaaggcga aaaagttgac ggcggcgttc
     6301 actatctgga agatccctat ctgccggaat tcggccttga cgactttccc cggctgaaga
     6361 aatttctgga tgtccatctc acggcaaaac cggaaatctg cccggaaatg ccgcggcttc
     6421 tcaccgagtg gcataaaaaa aacggttttg aaaccgacgc gaagggccgg ccctgggaac
     6481 cggcaacccg caaagccctt tcctacaaat atctgatgga aaaccgcgca cccgttattg
     6541 ccggagattg tctgctggcc ggaaccacca ccagccgcga ggtgggatgc atcgtgtacc
     6601 ccgagggaag cggcggcctg atctggaacg agctcttcac gattgctcat cgctcctaca
     6661 atccctttga gatctcggaa gaaacaagaa accttctgca ctacgaggta tttccctact
     6721 gggcccatcg caatttccgg gaatgggttc gggatcgata caacaatcct ctatgcatgc
     6781 agattgatga acgctatgcc tattatttca actggaaaca ggcaaccatc tcgcacacca
     6841 ttccggattt tccaaagatg ctgcgcctgg gcacatccgg catcatcggg gagatcgaac
     6901 aggaactcaa aaatgtgcag ggcaacgccg aaaagaaggc cacgctcgaa gcgatgattc
     6961 tcaccctgga aggcctcacg gcctactcga aaaatctggc cgcacaggcg aaaaaagacg
     7021 ccgcagtcca ggcggatccc gtccgaaaaa aggaactgga gagaattgcc gccgcctgtt
     7081 cacaggtcgt tgaaaaaccg gcgcgcagcc ttcacgaagc cgtccaggcc atgtggatca
     7141 cctgggtcgg gcttcacatg gagagcatga atgcgggcct ttcgctgggc cggctcgacc
     7201 agtggctcca gccctatttc ctcgccgaca tgcagcagct ctcttcccgg gaagaccgcg
     7261 cagaatacat tcgggatgcg atcgaactca tcgggcactt ctacatgcgc tgcacggacc
     7321 atttccctct cacgccggat ctggccaact tttattttgg cgggagctct tccgaccagg
     7381 ccatcacgct gggcggcgtc acgcccgaag gcttagacgc ggtcaacgac atgacctaca
     7441 tctttctgaa agtgaccgaa atgttaagcc tgcgcgatcc caacgtgaac gcgcgcttta
     7501 cgcccggcat cagcagcgat gcttatctga aacgcctctg cgaggtaaat ctgatcaccg
     7561 cggcaacgcc gtcgatgcac aatgacaagg ccatgttcgc ggccctgtct cccatggggt
     7621 atgatattcg ggatatccgc aactggtcgg caacgggctg cgtcgaaccc acgctttcgg
     7681 gcaagcacat ggcccacacc aacatgcaga tgatgaacat ggtcgccgcc cttgaaatgg
     7741 cgctcaacaa cgggctccac cccctcacgg actggaaact gggtccggaa accggcgcaa
     7801 tcgagcaagg cgacttcaat agctttgagg acttcttcaa cgcctttacc gagcagtttg
     7861 cctttctcat cgaccgctcc atcgaataca acgatctgct ggccctggcc catcagtacc
     7921 tgcgccccac gccgctgctc tcttccgtga tcgacggatg catcacaaaa ggaaaagacg
     7981 tcaccagggg gggggcaacc tacaacagtt ccggaaccgc cattgtcggc cttgccgatg
     8041 tcaccgattc cctgatgacc atcaaaaagc tggtttttga tgaaaagaag atctcgtttg
     8101 cgcatttaaa gaaggcggtg gacggcaact ttgaaaacga tcccgaactg ctggccatgg
     8161 tgcgcaggaa agttccgctt ttcggctcgg ggaatgcgga agcggtggag atggccaacc
     8221 gcctgacccg ctggtcgcac gatcattatg caggcctgac gcactacagg ggcggcaaat
     8281 ataccaccgg tttctggacg atgtcgcagc atgtggcgtt cggcattctg acgggcgcgc
     8341 tcccttccgg cagacaggcg ggcaaaccct ttacgccggg tctcacccct cagcccttcg
     8401 cttcgaaaaa cctgctcgac aacattcggg acgttgcgaa gctggatccc gtcaacctga
     8461 acaacaacat cgccttcaat gtcaaggtgg tgccgtcggc cgccgacaca cgtgaaaaaa
     8521 ccgtgcacaa catgttctcc tatgtaaaaa cctatttcga tctgggcggc atgcagatgc
     8581 agatgaacgt agtgacgtcg gccatgctgc gtgacgcaat ggcacaccct gaaaactacc
     8641 ggaacctcat cgtccggatt tccggctata acgcctactt tgtaaccctg aacaaagaca
     8701 tgcagcgtga gctgattgaa cgggcggaat atgcaatgtg acggggcggc catggcccag
     8761 acggaagaat cccaggcccg gaagcctctc attcttgaaa tcaaggggaa ctcgcttgac
     8821 gacggccccg gcattcgaac cgtcgttttt tttaaaggat gccccctgtc ctgttcctgg
     8881 tgccacaatc ccgaaagcaa gctcgctcgg gttgagatcg gttatgatcg caaagagtgc
     8941 atcggttgcg ggacctgcac ggccgtctgt ccggaaaaag cggtatcgcc gcaaaatatc
     9001 catcctgtgg atcgagaaaa atgcaccctt tgtttccgct gtgttgagga atgccccgcg
     9061 ggcgccctcg cccgcgtcgg acgaagcctg tccattgatg aaatcgcggc cgaagtctta
     9121 aaggacaaac cctttttcga cacctccgga ggcggcgcaa ccttttcggg gggcgagccg
     9181 gtaatgttta tggagtttct gtccgaattg ctccatgctt tgaaagcata tcaagttcat
     9241 accctgctgg aaaccagcgg cttttttgat tttgaaaaat tcgaaagatt gcttcttccc
     9301 tgtctggaca tgatttatta cgacatcaaa atcatggatg agaaagcgca tcgcattcac
     9361 acgggggttt ccaatcaacg aattctggaa aactttgaaa ggcttttcaa aatctccagg
     9421 gataccggga tcacgcttct gccccgaacg ccccttgtgc cggggatcac cgaccgccat
     9481 gagaatattc agggcattgc ccgattcctg gccgagctgg gcgtcaggaa cgcggcgctc
     9541 atgccgtaca atcccctctg gcatgaaaag gcggataaaa tcggcgcgga caatccccat
     9601 cgcggccatg ccgtcatggc ggcatggatg gagaaagaaa aggtggaggc ctgccggaga
     9661 atattttccg aagaaggcat tcaggtcgtt tagaggccgg atagataaag aagctcccgc
     9721 ccggcatcgg ggatttcggt cgaaaggtta tcttcaaact gcttctcccg tttcaggggg
     9781 agccattcac ccggttggcc ggactttcca tcgggtcggc gccatggaaa accggcataa
     9841 aagaaatcag gctttccgtg gatatggcgg ataaaatatt ctttacacaa acgggagggt
     9901 tttgagtata cttgacttca ttaaaaggga atttttgtca atgacccttc cggcggtcca
     9961 tctcccggcg aggaacttcc ggaaaataat ttttgaaggt gctcatggaa atcaagttta
    10021 aaatctggct ggaaaaagac ggtcatgccc tgttcggcga gggacgcgac cagttgctga
    10081 aagccatcga cgaatgtcat ggtctttacg gggccgcaaa aaaattgaaa atgtcctacc
    10141 gcgcggcctg gggcaaaatt aaattgacgg aagagcgcat cggcaagaaa atcgttgaaa
    10201 cgggagagga caagaagctg cacctgacaa aagacgccaa aaaaattctg gatgaatttg
    10261 aaaagctgga aaaagatgtc gatggttttc tccatgcgcg caggtccact tttatcgtgc
    10321 tgaaagatcc ccccacgaaa agcgacgaaa agaaatgagt attgaacgca tacccccccc
    10381 atcctccggc tatgctcctc cgggcatatg ctcacagatg cataactata tgaaataatt
    10441 gaaattattc cttgacaatc ccttaaaatc ctttatgcta aatccaacag aacaaccggc
    10501 gtcttcatga cgcaggatgt ccggacaaga aaattttgag tgtcggacga tgttcagagg
    10561 gactgaacgt tcggaagacc ttcaaagaat attttgccgg ttacatgtgt aaaggaggac
    10621 atttctatgg aagagaagtt ttcaatgaaa agaaggagtt ttttgaagct ggcgggagcc
    10681 gccggtgttg ccaccgcgat gacggctttt cccttcagga atatgcgggc ggcatgggcc
    10741 ttcggggatc atccccagga acagttgccg tatcgcatta ccaaaagagt cccgcaggtt
    10801 tgcgcaagag cctgcgaggc ggactgcgct tacaatgttg tggtcggcgt tgatcccgcc
    10861 accggtcttg aaagggccct gacgctggaa ggacgtccgg aagatcccgt gtccaacgga
    10921 aaattctgca tcaaaggcat ggggtttgtg gattcgatgt acgatccgga ccgcctgatg
    10981 gtttctctga agaggaccaa caagacgaag ggaaccgatg tggatcccgg ctggattatc
    11041 atgaagacgg aagatgcggt caacgaaatc atcgccagga tgaaaaccta caaaccggaa
    11101 gaaattctga tggcgtcgcc gggcgatcca tacaccaacc gactgtgtca gtccatcggc
    11161 tgcacccgga gcgatcagcg gacggaatgt ttcggcacac attactacat caactgcctg
    11221 acgctcacca atccgcccaa caagttttat tccagctgct acaccccgag ccatcatgtg
    11281 tgcggttatg actacgacac ctgcaagtac caggtctggt tcggatttga ctctttttcc
    11341 aagtgtggca aggcgggcat gctcaatcac tgggcggcgg gaaaaagaaa cggcggcaag
    11401 attgtcatgt tcaacccggt ccgcaccccc atgacggaca attttgccag tgagtattac
    11461 gcgatcaaac caggaacgga tctggccgtt gcgctggcca tgattaacca gattatggcc
    11521 ggcaagaagt acaacaagga attcatgacc aaatattcgg acggcgcggc tctggttgat
    11581 gtggaaaaac agacgcatct gaaaacagcc gacggcatgt ttctggcgtg gtccgtaaaa
    11641 aacaaaaaag ccgaacccat cgatgaatgt gatgatccgg cgcttgacgg ctcctatacc
    11701 gtaacgcttg acggacggca aatcaaggcc aagacggttc tgcagcttct ggccgaggcg
    11761 acaaagacct atacgcccca gtgggccgcg aagatcagcg acgtgccggc caaggccatc
    11821 agcaagatcg cccgcgattt tgccgccgcc gcgccctacg cgctgattcc cacgtacaag
    11881 cgcgacgcgg cgggccccaa ttacgccaac agctggcgtc ttcgccacgc catccagatt
    11941 ctgaactccc tggccggttc gatcgaccat gaaggcggca tgcttctgct gcacgacgtc
    12001 aagattccct ggatcgacga aatctccccg ccggcgttgc cttatcctga acagccgtcc
    12061 cagccggtgg acttccgcaa tgaatttccg gtcacggatg acatttaccg gaagaaggat
    12121 ttttcggcgc cgggtcatta cggcatggta ggatgggggc tttacaagac aaaccgcgcg
    12181 aaggttgcct ttttccggaa cccgcaccga gggctctttg ccatgatcca ttcccagatg
    12241 ttcgaggccg cctctcagaa actggaactc gtcgtggact ggaaccttta tctggacgat
    12301 ctgggctact tctgcgacta cgtagtaccg gcgcctcatc agtttgaaga gggcaaaatg
    12361 gacctgcgtc tctactatcc gaaatatccc tgcattgtcg gcggttcgcc ggtgcagaaa
    12421 tcgccgggag accagatcgg ctggggcggt ttcgccatga agatcggtct ggcgatggcg
    12481 ccgaagtact ggacgacgga cggcagcggc aatccggcca agaaaatccc gacaaatatg
    12541 ggcgatgtcg cagtcaagaa ggcgggcatt gccgacagcg ccgccgattt catgaagaag
    12601 ggcggtttgt ggatcgataa aaaagagtat cagaactaca agcagatcga gcagatcggc
    12661 tacggcaagc ccagaggaag ggtgaagctg tacatcaacg aatttgccga agtcggccac
    12721 agcccgctgc ccgtctggca tgcccgctgg catgaaacca cgggagacta caagttctcg
    12781 ctgctcatca cccgggcgcc ttggtatatg catgccgatc ccaatttcat caacaacccc
    12841 gtcctcaagg aagtgacggt ccgcaaccac atggatacgg catggatcaa tcccgaggct
    12901 gcggccaagc tgggtctcaa ggaaggcgac ggcgtcatca tcgagaacga tccgacctac
    12961 atgaaggatc ttcctcggcc acagaaagcc aaggtgcatc tgacgaagcg catcacaaga
    13021 aacgactgcg tcctgttgtt ccacggcatc gggcaccggg ccaagaacct gaaggtggcg
    13081 gccaatttcg gttaccggga cggcgatttg attccgcaga aggatccggc gatgttaaag
    13141 aagttcgatc ctaccggaat gggatgggtt gaagatgttt ttgtcagcat taagaagatg
    13201 tagcgaggtg acgaaatgaa aagatatgcc attttactgg atgatacgtt ctgcacgggg
    13261 tgcaattcct gcgcttaccg gtgcatccag gagttccggt atcacgatca ggcgtcaaag
    13321 ggtctgttca gaacctttgt ccaaatcaac gacgacggcc tttaccagaa gcgctgcatg
    13381 cattgtctgg agccggactg cgtcgaaaac tgcccggtga aggcgcttac caaatcggaa
    13441 tacggtccgg ttctctacga tgcgaagaag tgcatcggct gccagacctg cgtccgggtg
    13501 tgcaagtttc atgttcccca gtttgacgcg gatacgaaga agatcgtcaa gtgcagcatg
    13561 tgcgcgcacc ggatcaacga cgacaagaaa ccggcctgcg tggagatttg ccccaccggc
    13621 gcgctgcagt tcggcgaata tgaagcgatg aagaaactgg cgacaaagac ggccaaagag
    13681 aagaaactca aactgtacgg ctacgaggaa aacggcggaa cccatctgtt cgttctggcc
    13741 aaggaagatc ccgtgaagtt cggctatccc aaggtggcca agaaggccct caagggcaaa
    13801 gcagcgctgg atggagaact gggcgtgccg gctctcgcgg ccttggccgt tgtcggcttc
    13861 aagaagtaca gcgaaagaag aacccgtctt gaaacacgga cggagaagga aaaaaagtaa
    13921 cccaacccta aactgaaatc cggcggcgct ctgcaacgcc gccggatcgt ttgccgtttt
    13981 gtcggtcatt ggacaatccg gtggaaattt gttaggaagc aatcatgaac aaccaaacga
    14041 ccaaaacgcg ccggacgggt cctgacgacc gcctgatcga gctgcaggcc aaagcggcgc
    14101 ttttcaacct gctggcgcag gcatttgcct atccggatca gctcctcatg gaaaaactgg
    14161 tttcggggga atttgccgca atcctggacg aatcgctggg cgttcttcag ccggaaggcg
    14221 catgggccga tcggatcgcg gttgtgaagc gccaatacac cgccgccggt cagaacgccg
    14281 cggacctgct gctggagctg gaaaaagatt ataccagaat gttcttttcg tcaaagcccc
    14341 ggctggttta tcttttcgaa tccgtttaca aggaaggaaa actgcttcag gaatcgacct
    14401 ttgaaatcgc gaggctgtat tacgaagcgg ggctcaatct ggtggatgat ttcaaactgc
    14461 cgcccgatca catcgcggtc gagtttgaat ttatggccta cctgtatttc aacgaacttg
    14521 cagccgtgaa gagtgaaaac agggaaaacg aagcgcttgc gcagaagctt cagaaagagg
    14581 cgctggaaaa gcatctcacg aattttggac ggacgttggc ggaaaaggtc gtcacccatg
    14641 ccgcgacgga tttttataag atcatggcgg ccgtcgccgc ctccgtcctc gcggccggcc
    14701 ggtaggcaaa atataggttt tgactactct ttgatcgtaa a
//
LOCUS       DAOPAF010000036        13979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4384_length_13979_cov_30.519032, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000036 DAOPAF010000000
VERSION     DAOPAF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_13979_cov_30.519032"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLQ77_05070"
     CDS             <1..554
                     /locus_tag="PLQ77_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL10080.1"
                     /translation="DIMDVALNLIAERGFHDSPMALIAKEAGVAAGTIYRYFESKDEL
                     IIALHRDLESRINEMILKEYPAEESIREKFLYMNRKLLKYFMEHPLHFRYMEQFFNSP
                     YGMSHRKERLLEKSDRNRMMIDIFEQGTRQEILKALPMPALFALAFGPLPSLLRDHIF
                     GFIHLDEEMIEEVTQACWDAIKK"
     gene            570..1766
                     /locus_tag="PLQ77_05075"
     CDS             570..1766
                     /locus_tag="PLQ77_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL10081.1"
                     /translation="MQIYAIALRMIFISLLAAGFLLNGCSDKGKGGPPPPTEVSVVTI
                     QAKSVATTTELPGRTSAHLVAEVRPQVAGIIQKRLFSEGANVRAGQVLFQIDSSLYQA
                     ALDNAKASLARSEAQLSTIQLKAARLKELLAEKAVSQQDYDDAAAALKQTQADIQFGK
                     ATVEAARINLQHTAIGAPISGRIGRSSVTEGALVTTHQPQPMATIQELDPMYVDVTQS
                     TAEILRLRRQLQEGRIEHSMKHLDGVRLLLEDGSEYPWKGVLQFQDVTVDPTTGSVVL
                     RMVFPNPKGILLPGMFVRTVIQEGIRKNAILVPQQAVSRDLKGNPFAFVVDSKGIVQQ
                     RPLTLDRTVGDAWLATSGLESGERVIVEGILKVKPGAPVRAVSFKPSAKTNGKGQNPA
                     PPDQTR"
     gene            1778..5035
                     /locus_tag="PLQ77_05080"
     CDS             1778..5035
                     /locus_tag="PLQ77_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPL10082.1"
                     /translation="MLSKFFLDRPVFAWVIAIVVMALGVIAIYILPVSQYPQLAPPSI
                     AISSSYPGASSETVENSVTQVIEQKMTGLDNLIYMSASSDSAGGSRLELTFAPGTDPD
                     IAWSKVQNKVQSALPSLPDVVENQGVQVSKSTRNYLMIVGLISEDGSMDGTDLRDYAK
                     SNLEKIMARVPGVGEVEVFGGGYAMRVWLNPNEMTEYHLTVDDVLAALKAYNVEVSAG
                     QFGGAPAVPGQRLNASIVVQNMLSTPEEFAAIPIRTNPDGSVVRIRDVGRTELGTEIY
                     DIQVSYSGKPSAGIAIRQSPGANALDTADAVKAKMAEMSRFFPPGMKVVYPNDSTVFI
                     KVSINEVIKTLVIAIVLVFLVMLLFLGSIRATLIPTIAVPVVLLGTFAVLALFGFTVN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMTEEGLSPKEATAKSMEQITSALVGIGLVL
                     SAVFGPMAFFPGSTGIIYRQFSITIMSAMLLSVAVALILTPVLCAGLLKPVQAGHQPA
                     ENAASFARPFFLWFDRVFFRLRDRYVGLVGHSLSRKKRYLVVFLVIVAVMGVLFLRMP
                     RAYLPDEDQGILLAQVIMPTGATLEQTGEITTQIQKYFEENEKEAVESLMTITGIGFS
                     GRSQNNGMVFIRLKDWHLRDRADLKAKAIAGRAMFALSGIRNAMVFTFQPPSVIELGT
                     SIGFDFQLLDRGGVGHSALTNAQYQLLGLAAKDPRVAKVRPNSMEDVPEYRIDVDWER
                     AGALGVPISSIHRTLSAAFGSAYANDFIQGGRIKRVYIQADAPYRMLPKDLENLYVRN
                     TQGRMVPFASFATGRWTSGPPRLTRYNGFPSINIWGEPAPGRSSGEAMQAMEEAMEKL
                     PSGFGFDWSGLSYQERMAGTQAPLLYAFSIFVIFLCLAALYESWTIPISILLVLPLGV
                     IGGIIASSARGLANDVYFQIGLLTTLGLTTKNAILIVQFAKSGLERGMGLLEATLEGA
                     KLRFRPIVMTSLAFGFGVLPLAMTTGAGAGAQNAIGTSVLGGMITATLLVVIFSPLFY
                     VLIETLFGRKLKTDAGKTGAPSLFEHLRQTILTGRRKKKSSRTDKADASEVEQHE"
     gene            5028..6422
                     /locus_tag="PLQ77_05085"
     CDS             5028..6422
                     /locus_tag="PLQ77_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPL10083.1"
                     /translation="MNKKWLLILMVLLAGCTMAPKYERPEAPVPNEWPAGQSYGEEKQ
                     ALETTQPGWREFITDQRLREIIADSLKNNRDLRVAALNVDKARAMYGIGRASLLPSIN
                     ASGVWYRERIPADLSSSGNSVTAEQFNVSLGMASWEIDFFGRIRSLKDQALEQYLATD
                     QASRSAQILLVAAVAQVYWTLAADREALSLVSSTLSAQEAAYKMIRKRHEVGLASELD
                     LRRAQTQVDTARGDVARYTQMVAQDENGLNLLIGSPVRPDLLPRDLSGVALPRDISAG
                     LSSEPLLRRPDVLAAEHRLKAANANIGAARAALFPRISLTTSIGTASAELSGLFKAGS
                     DVWTFAPQIVMPIFDPRVWYAYDVTKIEKEISIAQYEKSIQTAFREVADALAEKGMVN
                     ERIAAQQSLVDALAVAYRLSELRYEKGIDSYLSVLDAQRSLYGAQQGLILLRLASINN
                     RVALYKALGGGASP"
     gene            6419..6856
                     /locus_tag="PLQ77_05090"
     CDS             6419..6856
                     /locus_tag="PLQ77_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HPL10084.1"
                     /translation="MTKKRRPCSAKEIKYFLLNRVLTSAIVFVLRIYAKTLRVRIEGK
                     EEILKHLAGGGRVIFASWHQRLFGGFFVPRFFQWTPCIMISQSRDGDFISKIVSRLGW
                     IPVRGSSTRGGAKALKAMPRTAAGENDEKTQTRKPNLTNSTWI"
     gene            complement(7028..7753)
                     /locus_tag="PLQ77_05095"
     CDS             complement(7028..7753)
                     /locus_tag="PLQ77_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPL10085.1"
                     /translation="MLVKSKISAHHFPQGVCLSCGEGNIGKRRYCSVRCRQHLRQRLN
                     TRSGLLQALNTRYATFYFTDTLIVLDVVPHGIREVFRFQTKRTAGTKPADDFGRMSNL
                     LGDAWWAEEKRTTRNYLASRRVLEMAERLAMADGLKRPRWMKAPCVKPESILLLEMSK
                     ADLASRDLDKIIRNAYRRQAKIHHPDAGGTAAAFRRLHAAYQDLLRWADHPTFIRRRG
                     FPDKWYYDGDNKKWIQPVPVRKG"
     gene            complement(7814..8425)
                     /locus_tag="PLQ77_05100"
     CDS             complement(7814..8425)
                     /locus_tag="PLQ77_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPL10086.1"
                     /translation="MIEKNVKTDFSATVSEPVIGEGTYIHPLAAVIGNVELGKNIMVS
                     PMASVRGDEGQPLHVGDDSNIQDGVIIHALETELDGKPVEKNKMEVNGRKYAVYVGNR
                     VSLAHQVHIHGPAVVMDDTFVGMRALVFKSFVGKNCVIEPGAILIGVRVADGRYVPGG
                     SVVKTQADADALPVITADYPMKEMNKGVLHVNKALARGYLAAR"
     gene            complement(8508..9122)
                     /gene="coaE"
                     /locus_tag="PLQ77_05105"
     CDS             complement(8508..9122)
                     /gene="coaE"
                     /locus_tag="PLQ77_05105"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPL10087.1"
                     /translation="MLNVGLTGGIACGKSTVAEMFVCLGGHLIDFDKLAHEVQEPEKP
                     AWRDVVQCFGTEILLPDRTIDRGKLASIVFCSPEKLRRLNSIVHPRVFEEWWSRLEKI
                     KAQTPHAVVFSDVPLLFEGGMQHLFELTILVVVEPECQIERLMTRNSVCREEAQLRLA
                     SQMPIGEKIRLATIVIDNQCDPKETEKKVAAAWEDLVARERAKP"
     gene            complement(9126..9902)
                     /locus_tag="PLQ77_05110"
     CDS             complement(9126..9902)
                     /locus_tag="PLQ77_05110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPL10088.1"
                     /translation="MLLVIDVGNTNTVLGLFDQDQLLHDWRIRTEIDHTIDEYGVLIY
                     NLYQSIRMKAKEIKSVRSIIISCVVPPMLNILEPLCIKYFNIKPMIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAFEKYRQETIIVDFGTATTFDYVSPRGEYMGGCISPGIVIS
                     SEALFERASKLPRVEFGKPRTIITKDTVSAIQAGIMFGYAGLVDGIVNRMKAEVKSNP
                     LVIATGGLARVVAQEARTIDKIEEMLTLDGLRIIYERNKK"
     gene            complement(9909..10700)
                     /locus_tag="PLQ77_05115"
     CDS             complement(9909..10700)
                     /locus_tag="PLQ77_05115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPL10089.1"
                     /translation="MPLNQSSLKQSLAGRFIGHTLYYYEEIGSTNDEVFRLGMQGAPE
                     GTAVIAERQSAGKGRMQRTWHSPPGANIYTSILLRPRFSPGLAPQISLAAGVAVAKTL
                     EPYCPGRVFLKWPNDVQIGGKKVCGILTQMRTAGDAVDFVVAGIGVNVNWKRKEFPED
                     IRGIATSLSMEAGREISRLELIILLYENLAKCYRELAQKGFAPVRKQWLERTAMLGKP
                     VSVQFGKETIRGEAVDLDEDGSLVLLAEGQKMVKVAAGDATVGKE"
     gene            complement(10790..11632)
                     /gene="nadC"
                     /locus_tag="PLQ77_05120"
     CDS             complement(10790..11632)
                     /gene="nadC"
                     /locus_tag="PLQ77_05120"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPL10090.1"
                     /translation="MVSPQIKIIIESALAEDIGTGDITTQATVSPKKKGRAVAVAKEN
                     FVVAGLEVFEAAFQCLDKNIKVAKLIGDGRRAKKGDVIAEVTGSLSNILQAERVALNY
                     FQRMCGIATLTAAYVNAVGKAKAKIYDTRKTVPGLRVLDKMAVRVGGGTNHRMGLYDA
                     VLIKDNHIEAAGGITAAVAAQRKHLKRSLTIEVETKNLREVREALACGADIIMLDNMT
                     IGAMKKAVALIAGRALTEASGNVTLRRVADIAATGVDIISVGELTHSVRAADISLKVR
                     GEGR"
     gene            complement(11875..>13979)
                     /locus_tag="PLQ77_05125"
     CDS             complement(11875..>13979)
                     /locus_tag="PLQ77_05125"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPL10091.1"
                     /translation="AISDLEVEFKADQSNLWNIRYPFADGSGEIVVATTRPETMLGDT
                     AVAVHPDDPRYRDKIGKMVILPLMNKPIPIIADDYVTMDFGSGAVKITPGSDPNDFAM
                     AQRHNLEIISVIDGEGIINEYGGSYKGQDRYEARDNVIADLEKGGYLVGIEPYAHNVG
                     KCYRCKTDIEPMVSKQWFVKIRPLAAKAIAAVTKKKTRIVPASWEATYFEWMNNIRDW
                     CISRQLWWGHRIPVWYCGGCGKVIVSTVDPEKCPVCDGTSLKQDEDVLDTWFSSALWP
                     FTTLGWPKRTQALKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDVPFKDVYLHALV
                     RDEKGDKMSKSSGNSIDPLVMIDRYGTDAFRFTLAAYTAQGRDVRMSPERIEGYKFFV
                     NKIWNAAKLTLSNLADYDENGSLNAPDALPEKWIKAKLNEAIDEVTRSLDEYRFNDAA
                     ASIYNFIWHEYCDWYLEVSKPALYGKLSPERKQATQRTLKEVFAVMLQLLHPFMPFVT
                     EELWQTLLGSDENSIMVSRFPVVNDAWQEKDARQEMELLMEIITSVRNIRGEMRIPPS
                     VKLKVLISAVDEAIKTSVENGAAYIVNLAALESLTVDVNLVEPKGVATGVIGSTKVFV
                     PLAGIVDIAGEKARLEKELAKVAKDLEQSSRKLANRDFRDKAAPEIIVKEEGKLKSFQ
                     EKFSALEGALKKLKEVTV"
BASE COUNT         2799 a         4223 c         3854 g         3103 t
ORIGIN      
        1 ccgacatcat ggacgtggcc ctgaatctga ttgccgagcg gggttttcac gattcaccca
       61 tggccctcat tgccaaggag gcgggggtgg cggccggaac catttaccgc tattttgaaa
      121 gcaaggacga actgatcatc gccctgcacc gcgatctgga atccagaatc aatgaaatga
      181 ttcttaaaga atatccggcc gaagaatcca ttcgggaaaa atttctttac atgaacagaa
      241 aactcctgaa atatttcatg gaacatcccc ttcatttccg ctacatggag cagtttttca
      301 actcccccta cggaatgtcc caccgcaagg aacggctttt ggagaaatcg gacagaaaca
      361 ggatgatgat cgacatattc gagcagggca cgcggcaaga gattctcaaa gccctgccga
      421 tgcccgcgct gtttgccctg gctttcggtc cgctgccctc gctgctgcgc gatcatattt
      481 ttggttttat tcatttggat gaagaaatga ttgaagaggt tacccaggct tgctgggatg
      541 ccatcaaaaa ataaaaacag aggtgccgaa tgcagattta cgccatcgcc ttacgaatga
      601 tcttcatcag tcttctcgcc gcgggatttc tcctgaacgg ctgttcggac aagggcaagg
      661 gagggccccc tcccccgacg gaagtttccg tggtgaccat tcaggcaaag tccgttgcca
      721 ccacgaccga actgccggga aggacttcgg cccatctggt cgcggaagtg cgcccccagg
      781 tggccggcat catccagaag cgcctcttct cggaaggcgc caatgtccgg gccggacagg
      841 tgctttttca gatcgattcc tccctgtacc aggcggcgct cgacaacgcg aaagcctcgc
      901 ttgcgcgctc ggaagcgcag ttgtccacca ttcagctcaa agccgcccgc ctgaaggaat
      961 tgctggccga aaaagccgtg agccagcagg actatgatga cgcagccgcc gcgctgaagc
     1021 agacccaggc ggatattcag ttcggaaagg ccaccgtgga agcggcccgc atcaacttgc
     1081 agcacaccgc cattggggcg cccatttccg ggcgcatcgg ccggtccagc gtcacggagg
     1141 gcgcgctggt gaccacgcac cagccgcagc ccatggccac gattcaagag cttgatccca
     1201 tgtatgtgga tgtcactcag tccacggctg aaattcttcg cctgcgccgc cagcttcagg
     1261 aaggccgcat cgaacacagc atgaagcacc tcgatggcgt ccggcttctg ctggaagacg
     1321 gctcggagta tccctggaaa ggcgtccttc agtttcagga cgtcaccgtc gatccgacca
     1381 ccggatcggt tgtcctgcgc atggtatttc ccaatccgaa aggcattctg ctgccgggca
     1441 tgttcgtccg cacggtcatt caggagggaa tccgcaaaaa cgcgatcctc gtcccccagc
     1501 aggcggtttc ccgcgatctc aagggaaatc cttttgcctt tgtcgtggat tcgaaaggaa
     1561 tcgtgcagca gcggcccctc acgctggacc ggacggtcgg cgacgcatgg ctggcaacct
     1621 cgggcctgga atccggagag cgcgtcattg tcgaaggtat tttgaaagta aagcccggcg
     1681 cccccgtcag agccgtttct tttaaacctt ccgcgaaaac gaacgggaaa ggccaaaacc
     1741 cggccccgcc cgatcagacc cggtaacgga ggcgtaaatg ctatccaaat tctttctgga
     1801 ccgtcccgtt ttcgcctggg tcatcgccat cgtcgtcatg gcgctgggcg ttatcgcgat
     1861 ctatattttg cccgtctccc agtatccgca gctggcgcct ccctccatcg cgatttcatc
     1921 ctcctacccg ggagcctcgt ccgaaacggt tgaaaacagc gtgacgcagg tcatcgagca
     1981 gaagatgacc ggtctggaca atctgatcta catgtccgcc tccagcgatt cggcgggcgg
     2041 ctcccgtctg gagctgacgt tcgctccggg aacggacccg gacattgcct ggtccaaagt
     2101 gcaaaacaag gtccagtccg ctctgcccag ccttcccgac gtcgtcgaaa atcagggagt
     2161 gcaggtcagc aaatccacac ggaactatct gatgatcgtc gggctgattt cagaagacgg
     2221 ctcgatggac ggaacagacc tgagggatta cgccaagtcc aacctggaaa aaattatggc
     2281 ccgcgtcccc ggcgtcggcg aggtggaggt tttcggcggc ggctatgcca tgcgcgtctg
     2341 gctcaatcct aatgaaatga cggagtatca tctgacggtt gacgatgttc tggccgcgct
     2401 caaggcctac aatgtggagg tatccgccgg ccagttcggc ggggcgcccg ccgttcccgg
     2461 ccagcgcttg aacgcgtcca tcgtcgtcca gaacatgctt tccactccgg aagaatttgc
     2521 cgccatcccg attcggacca atcccgacgg ctccgtggtt cgcatccgcg acgtgggacg
     2581 aacggaattg ggaacggaga tttacgacat tcaggtttca tacagcggca agccttcagc
     2641 gggcatcgcc atccgccagt cgccgggcgc gaacgcgctc gacacggccg acgcggtcaa
     2701 ggcgaaaatg gccgagatga gccgcttttt ccctcccgga atgaaagtcg tttatccgaa
     2761 cgactccacg gtcttcatca aggtgtccat caatgaagtg atcaagacgc tcgtgatcgc
     2821 cattgtgctg gtgtttctgg tcatgctgct ttttctgggc agcatccgcg ccacgctgat
     2881 ccccaccatc gccgtgccgg tggttcttct cggaaccttc gccgtgctgg cgcttttcgg
     2941 ctttaccgtc aacatgctga cgatgttcgc catggtgctg gcgatcggcc tgctggtcga
     3001 tgacgcgatt gtcgtcgtcg aaaatgtgga gcgcatcatg acggaggaag ggctttcccc
     3061 caaggaagcc acggccaaat ccatggagca aatcaccagc gcgctggtcg gcatcggtct
     3121 ggtgctctcc gccgtcttcg gcccgatggc gttttttccc ggctccaccg gcatcatcta
     3181 ccgccagttc tccatcacga tcatgtcggc catgctgctt tcggtggccg tcgccctgat
     3241 tctgacgccc gtactgtgcg ccgggctcct caagcccgtc caggccggac atcagccggc
     3301 ggaaaacgcc gcctctttcg cccggccgtt tttcctgtgg tttgaccgcg tttttttcag
     3361 actgcgcgac cgttacgtcg gactggtcgg ccattcgctt tcccggaaaa aacgctacct
     3421 ggtcgtcttt ctggtcatcg tcgcggtcat gggcgttttg tttctgcgca tgcccagggc
     3481 ctacctgccg gatgaagatc agggaatcct gctggcacag gtcatcatgc ccaccggcgc
     3541 cacgctggaa caaaccggag aaatcacgac gcagatccaa aaatactttg aggaaaatga
     3601 aaaagaagcc gtggaatcgc tgatgacgat caccggcatc ggattttccg gacgctcgca
     3661 gaacaacggc atggtattta tccggctgaa ggactggcat ctgcgcgacc gggcggatct
     3721 gaaagcgaag gcgattgccg gccgggcgat gttcgccctt tccggtatcc gcaacgccat
     3781 ggtgtttacc ttccagcctc cgtccgtcat tgaactgggc acgtccatcg ggttcgattt
     3841 tcagctcctt gaccgcggag gggtggggca ttcggcactg acgaacgccc agtaccagct
     3901 tctcggcctc gcggcaaagg atccgcgggt tgccaaagtc cggcccaaca gcatggaaga
     3961 cgtcccggaa taccgcatcg acgtggactg ggaaagagcc ggcgcactgg gggttcccat
     4021 ctcatccatc cacaggacgc tgtccgccgc cttcggcagc gcctacgcca acgacttcat
     4081 tcagggcggg cgcatcaaac gagtgtatat tcaggccgac gccccgtacc gcatgctgcc
     4141 caaagatctg gaaaatctct atgtccgcaa tacgcagggc cgaatggtgc cttttgcatc
     4201 ctttgccacg ggccgctgga cgtcggggcc gccccggctc acgcgctaca acggctttcc
     4261 ttccatcaac atctggggag aaccggcccc gggacgcagt tcgggagaag ccatgcaggc
     4321 gatggaggag gccatggaaa aacttccttc cggcttcggc tttgactgga gcggcctttc
     4381 ctaccaggag cggatggccg gcacccaggc gccgctgctg tacgcctttt ccatttttgt
     4441 gatttttctg tgtctggccg ctctgtacga aagctggaca atccccattt cgattctgct
     4501 cgtgcttccg ctgggcgtca tcggcggcat tatcgcgtcc agcgcgcgcg ggctcgccaa
     4561 tgacgtgtat tttcagatcg gactcctcac cacactgggg ctgaccacca aaaacgccat
     4621 cctgatcgtc cagttcgcca agagcggtct ggagcgcggc atggggcttc tggaggcgac
     4681 cctggaaggg gcgaagctgc gctttcgtcc gatcgtgatg acgtcgctgg cctttggatt
     4741 cggcgtcctg ccgctcgcca tgactaccgg ggcgggcgcg ggcgcgcaaa acgccatcgg
     4801 caccagcgtg ctgggcggca tgatcacggc aaccctgctg gtggtgattt tttcgccgct
     4861 gttttacgtt ctgattgaaa cgttgttcgg cagaaagctg aaaacggacg cgggtaaaac
     4921 cggcgcgcct tctctttttg aacatttacg gcaaaccatt ttgaccggac gccggaagaa
     4981 aaaatcaagc cgtacggata aagcggatgc ttcagaggtt gagcaacatg aataaaaaat
     5041 ggcttttgat cctgatggtt cttttggcgg gctgcacgat ggcgccgaaa tacgagcgtc
     5101 cggaagcccc ggtgccgaac gagtggccgg caggccaatc ttatggggaa gagaaacagg
     5161 cattggagac gactcaaccg gggtggcgcg aatttatcac cgatcagcgc ctgcgggaaa
     5221 tcatcgccga ctctttgaaa aacaaccgtg atctgcgggt cgcggcgctg aacgtggaca
     5281 aggccagagc catgtacggc atcggacggg cctcgctgct gccttcgatc aacgcctcgg
     5341 gcgtctggta tcgggaaaga atcccggccg atctttcctc ttccggcaac tccgtcaccg
     5401 ccgaacaatt taacgtcagt cttggcatgg cttcctggga aatcgacttc ttcggacgca
     5461 tccgcagcct gaaagaccag gccctggaac aatacctggc cacggatcag gcaagccgca
     5521 gcgcgcagat tctgctggtg gccgcggtgg cgcaggttta ttggacgctg gcggcggacc
     5581 gggaggccct gagcctcgtt tcctccactc tttcggcgca ggaagcggcc tacaaaatga
     5641 tccgcaagcg tcatgaagtc ggactggcct cagagctgga tctgcgccgg gctcagaccc
     5701 aggtggatac ggccagaggg gatgtcgccc gctacacgca aatggtcgcg caggatgaaa
     5761 acggtctgaa tcttctcatc gggtctcccg ttcggccgga tctgcttccc cgggatctgt
     5821 ccggcgtagc tctgccccgg gatatttcag ccggcctctc ttccgaaccg cttttgcggc
     5881 ggccggacgt tctggcggcg gagcaccggc tcaaagcggc caacgccaat atcggcgcgg
     5941 cccgcgcggc tctctttccc cggatttcgc tgacgacgtc cattggaacc gccagcgccg
     6001 aactgtccgg gcttttcaaa gccggctcgg acgtgtggac ctttgcgccg cagatcgtga
     6061 tgccgatttt cgatccccgc gtctggtatg cctacgacgt caccaaaatc gaaaaggaaa
     6121 tttccatcgc ccagtatgag aagtccattc agacggcctt tcgggaagtg gccgacgcgc
     6181 tggcggaaaa aggcatggtc aatgagcgca tcgcggcgca gcaatccctt gttgatgctc
     6241 tggccgtcgc ctaccgcctc tccgagctgc gctacgaaaa gggcatcgac agctacctct
     6301 ccgttctcga cgcgcagcgc tcgctgtacg gcgcgcagca gggcctgatc ctgctgcgtc
     6361 tggcttccat caacaatcgc gtggccctct acaaagcact gggcggaggc gcgtctccat
     6421 gacgaaaaag cggcgcccct gttcggcaaa ggaaatcaaa tattttctgt tgaatcgggt
     6481 tctgacgtcc gccattgttt tcgttttgcg gatttacgca aaaacgctcc gcgtgcggat
     6541 cgagggcaag gaagaaattc tgaagcatct ggccggcggg ggccgggtca tctttgccag
     6601 ttggcaccag cgccttttcg gaggtttttt tgttccgcgg ttttttcaat ggacgccctg
     6661 catcatgatc agccagagcc gcgacggaga cttcatttcc aaaatcgtct cccgcctcgg
     6721 ctggattccg gttcggggct ccagcacccg gggcggcgcg aaagcattga aagccatgcc
     6781 gcggacggcg gctggggaaa atgacgaaaa aacgcaaacc cgcaaaccta atttgacaaa
     6841 ttcaacctgg atctgacact caactgcgac ggggagccgc tctctctcac caactaccgg
     6901 tttattgggt tgctaaaaca aaatgtgcga aagattgttg acactacccg aaaacagaca
     6961 accgatgctg aaatgtcaag attaaagatt tgacccctta ccctaaagat ttgacccctt
     7021 acccccctta cccttttctc accggcaccg gctgaatcca ttttttgttg tcgccgtcgt
     7081 agtaccattt gtcgggaaag ccgcgtcggc ggatgaaggt gggatgatcg gcccagcgca
     7141 ggaggtcctg gtaggcggcg tgaagccggc gaaaggcggc ggccgtcccg ccggcatcgg
     7201 gatgatgaat tttcgcctgg cggcggtagg cgttgcggat gatcttatcc agatcgcggg
     7261 atgcgagatc cgctttggac atctccagaa gcagtatgct ttccggtttg acgcaaggcg
     7321 ccttcatcca ccgggggcgc ttgagtccgt cggccatggc cagtctttcg gccatctcca
     7381 gaacgcgcct tgaagccagg tagtttcgtg tggtcctctt ttcctcggcc caccaggcgt
     7441 cccccagcag attgctcatc ctgccgaaat catcggccgg ctttgtcccc gccgtgcgct
     7501 tcgtctggaa gcgaaacacc tcccgaatgc cgtgcggaac cacgtcgaga acaatcaacg
     7561 tatccgtaaa ataaaatgtt gcgtagcgcg tgttcagggc ctgcagcagt ccgcttcgcg
     7621 tattgagcct ctgccgcaga tgctggcggc atcggacgga gcagtagcgg cgcttcccga
     7681 tgttgccttc gccgcaggaa aggcaaacgc cctggggaaa atgatgggcg gatattttgc
     7741 ttttgaccag catgcgtgaa tcagccggcg ggttgaaaaa cctttcctcc ggccttgact
     7801 ttccttcaat gatttaccgg gccgccaggt atcccctggc cagcgccttg ttgacatgca
     7861 gaacgccttt gttcatctcc ttcatcggat aatcggcggt gatcacgggc agcgcgtcgg
     7921 cgtcggcctg ggttttgacc accgatccac ccggcacata tcgtccgtcg gccacccgga
     7981 cgcctattaa aatagcgccc ggctcaatga cgcagttttt tccgacaaag gatttaaaaa
     8041 ccagcgccct catgcccaca aacgtgtcgt ccatcacgac ggccggtccg tgaatatgca
     8101 cctggtgcgc cagcgacacg cggtttccca catacaccgc gtatttcctg ccgttcactt
     8161 ccattttatt tttttccacg ggctttccgt ccagttccgt ttccagcgcg tgaataatca
     8221 cgccgtcctg gatattggaa tcatctccca cgtggagcgg ctggccttcg tcgccgcgca
     8281 cggacgccat cggcgagacc atgatgtttt ttcccagttc cacattgccg atgaccgccg
     8341 ccaggggatg aatatacgtg ccctccccga tcaccggctc ggacacggtc gcgctgaaat
     8401 cggtttttac gtttttctca atcatgcttg ccctccgctt ctctgtaata ggtccgcgcc
     8461 ggcttgtccg gaatcatgcg gcgcgtgctg atgtttctct tttttcttca aggcttcgcc
     8521 ctttccctgg ccaccagatc ttcccaggcg gccgccacct ttttttccgt ctccttcgga
     8581 tcgcattggt tgtcgatcac gatcgtcgcc agccggatct tttcgcctat cggcatctgg
     8641 ctggccagcc gcagttgagc ttcttcccgg cagacggaat tgcgcgtcat caggcgttca
     8701 atctggcatt cgggctcgac cacgaccaga atcgtcagct caaacaggtg ctgcatgccg
     8761 ccttcaaaca ggagaggcac gtcggaaaaa acgacggcgt gaggggtctg cgccttgatc
     8821 ttttcgaggc gggaccacca ttcctcaaac acgcggggat gaacgatgga gttgagacgc
     8881 ctgagctttt ccggagagca aaaaacaatg gacgccagtt tgccgcggtc aatggtccgg
     8941 tcgggcaata ggatttccgt tccgaagcac tgcacgacat cccgccacgc gggtttttcc
     9001 ggctcctgca cttcgtgggc cagcttgtcg aaatcaatca gatgaccgcc caggcacaca
     9061 aacatttccg caacggttga ttttccgcag gcaatcccgc ccgttaatcc gacattcagc
     9121 atgcgttact tcttattgcg ttcgtagatg atccgcaaac catccagcgt cagcatctcc
     9181 tcaatcttat cgatcgtcct ggcttcctgc gccactaccc gcgccagccc ccccgtggcg
     9241 atgaccagag ggttgctttt gacttccgcc ttcatccggt tgacaatgcc gtccaccagg
     9301 cccgcgtagc cgaacatgat gcccgcctga atggcgctga cggtgtcctt ggtaatgatg
     9361 gtcctgggtt tgccgaattc gacgcggggc agcttggaag ccctctcaaa aagcgcctcg
     9421 ctggagatca cgatccccgg cgaaatgcag ccgcccatgt attcgccccg gggcgacaca
     9481 tagtcaaacg tcgtcgccgt cccgaaatcg acaatgatcg tttcctggcg gtacttttca
     9541 aaagcggcca cggcattcac gatccggtcc gcgccgactt ccttgggatt atcataaaag
     9601 atcggcatgc ccgtcttaat gccggggccg acgatcatcg gcttgatatt gaaatacttg
     9661 atgcacaggg gttccagaat attcagcatc ggcgggacga cgcaggaaat gataatcgac
     9721 cgaacggatt taatctcttt cgccttcatg cggatagact ggtacagatt gtaaatcagc
     9781 acgccgtact catcgatggt atgatcgatt tccgtccgaa tccgccagtc atgcagcagt
     9841 tgatcctggt caaaaagccc caggacggta ttggtattgc cgacatcaat caccagcagc
     9901 atgcatcctt actccttccc gacagttgcg tctcccgccg caaccttcac cattttttgt
     9961 ccctccgcca gaagcaccag ggaaccgtct tcatccaggt caaccgcttc tccgcggatc
    10021 gtttccttcc cgaattggac cgaaaccggc ttgcctagca tcgcggtccg ctccagccac
    10081 tgctttcgaa ccggagcaaa acctttttga gcgagttccc tataacattt tgccaaattt
    10141 tcatacagga gaatgatcag ctccagtcgg gaaatttccc ggccggcctc catcgacagg
    10201 gatgtggcga ttccccgaat atcctccgga aattctttcc gtttccagtt gacgttcacg
    10261 ccgatgcccg ccacgacgaa atcgacggcg tcccccgccg ttctcatctg cgtcagaatg
    10321 ccgcacacct tctttccgcc gatctgcaca tcatttggcc atttcaggaa aacgcggccc
    10381 ggacaatagg gttcaagcgt ttttgcaacg gccacgcccg ccgcgagcga aatctgcggc
    10441 gcaaggccgg gtgaaaagcg cgggcgcaaa aggatggacg tgtaaatgtt cgcgccggga
    10501 ggcgaatgcc aggtccgctg catccttccc tttcccgcgc tctggcgctc ggcgatcacc
    10561 gcggtccctt ccggcgcccc ctgcatgccc agccggaaca cttcgtcgtt ggtcgagccg
    10621 atttcttcgt aatagtacag cgtgtggccg atgaatcgac cggcaaggct ttgcttcagg
    10681 gatgactggt tcaaaggcat aaagttaaga gttttaagtt ttaagttaag ggttgttcgc
    10741 tgtcggaacg gttgtgactc aattcctgct tcagccttca gcctatcgcc tatcgcccct
    10801 cgcctcttac cttcaacgat atatcggccg cgcggacgga atgggtgagt tctccaacgg
    10861 aaatgatgtc gacgcccgtt gcggcgatgt cggcgacgcg ccgcagggta acgtttccgg
    10921 aagcctcggt gagcgcgcgg cccgcgataa gcgccacggc ctttttcatg gcgccgatcg
    10981 tcatgttgtc cagcatgatg atgtccgcgc cgcaggccag ggcttcccgg acttcccgca
    11041 ggtttttggt ttccacctcg atggtgagtg accgcttcaa atgctttctc tgggcggcaa
    11101 ccgccgcggt gatgccgccc gcggcttcga tatggttgtc cttgatcaga acggcgtcat
    11161 acaggcccat gcggtgattg gttccgcccc ccacccgaac ggccatcttg tccagcaccc
    11221 gaagacccgg cacagtcttt cgcgtgtcat aaatcttcgc cttcgctttg ccgacggcat
    11281 tcacgtaagc ggccgtcagc gtcgcaatgc cgcacatgcg ctggaaataa ttcagggcaa
    11341 cgcgttccgc ctgcagaata ttggacagac tacccgtgac ttcggcgatg acatcgcctt
    11401 tcttggcccg tctcccgtct ccgatcaatt ttgcaacttt gatatttttg tccaggcatt
    11461 gaaacgccgc ctcgaaaacc tccaggcccg cgacgacaaa attctccttg gccaccgcga
    11521 ccgccctgcc cttctttttc gggctgacgg tggcctgcgt tgtgatgtcc cccgtgccga
    11581 tatcttcggc cagcgcagat tcaatgatta ttttaatttg aggagaaacc atttcttata
    11641 ccaaaacaat ctgtgtgatt tgtaattgac gggaggtgca atcgcttttc accctccctc
    11701 aatccctccc ctcaagggag ggaagaaagg caaccgcagg gagcggcttg taacctcgtg
    11761 tgcccttcag ggcatcaggc tatcacgacc gttccctgca aaacccgcaa tcgggcagaa
    11821 atccaaccac aggaaacggt tttaaaccac cttcgcgggt ggagcgaccg ttccctacac
    11881 cgtcacctcc ttcaatttct tcaacgcccc ttccagggcg gaaaattttt cctggaagct
    11941 tttcagtttg ccttcttcct tcacaatgat ctcgggcgcg gccttgtcgc ggaaatcccg
    12001 gttggccagc ttccgggagc tttgctccag atctttggcc actttggcca gctctttttc
    12061 cagacgcgcc ttttctccgg caatgtccac aatgccggcc agcggaacaa aaaccttcgt
    12121 cgaaccgatc acgccggtgg caaccccttt gggttcaacc agattcacgt ccaccgtcag
    12181 ggattccaga gcagccagat tgacgatgta ggccgctccg ttttccacgg aggttttgat
    12241 tgcctcatcc acggcggaaa tcaggacttt cagcttcacg gaaggcggaa tccgcatttc
    12301 cccccgaatg ttccggacgg aggtgatgat ttccatcagc agctccattt cctgcctggc
    12361 atctttttcc tgccaggcgt cgttgaccac gggaaaccgg ctgaccatga tcgagttttc
    12421 gtcgctccct aagagcgtct gccagagttc ttcggtgaca aacggcatga aggggtgcaa
    12481 aagttgcagc atgacggcga aaacctcttt cagggtcctc tgggtcgcct gcttgcgctc
    12541 agggctgagt ttgccgtaca gagcgggttt gctcacctca agataccagt cgcagtattc
    12601 atgccagatg aaattgtaga tggaagccgc cgcgtcgttg aaacggtatt catcgaggct
    12661 tcgggtcacc tcgtcgatgg cctcgttgag ttttgccttg atccattttt ccggcagcgc
    12721 gtcgggcgcg ttcagtgagc cgttttcatc gtaatccgcc agattggaaa gcgtcagttt
    12781 ggcggcgttc cagattttat tgacgaagaa tttatagcct tcgatgcgct ccggcgacat
    12841 gcggacatcg cgtccctggg cggtataggc ggccagcgtg aagcggaaag cgtccgtgcc
    12901 gtaccggtcg atcattacaa gcgggtcgat gctgtttccc gaggacttgc tcatcttgtc
    12961 gcctttttca tcgcgcacca gcgcgtgaag atagacgtct ttaaacggca catccttcat
    13021 gacataaagg cccatcatca tcatgcgggc cacccagaaa aagagaatgt cgaacccggt
    13081 gatgagcaga gaggtcggat aaaatgtttt gagcgcctgc gttcgcttgg gccagcccag
    13141 cgtcgtgaat ggccacaggg ccgaactgaa ccaggtatcg agcacatcct catcctgttt
    13201 cagggacgta ccgtcacaga cggggcattt ttccgggtcc accgtggaaa cgatcacctt
    13261 gccgcagccg ccgcaatacc agaccgggat gcggtgaccc caccacagtt gccgcgagat
    13321 gcaccagtcg cggatgttgt tcatccattc aaaatacgtg gcctcccagg aggcgggcac
    13381 gatgcgcgtt ttctttttcg tgacggcggc gatggccttg gcggccagcg gcctgatttt
    13441 gacgaaccac tgtttggaca ccatcggctc gatgtccgtt ttgcagcggt agcacttccc
    13501 gacgttgtgc gcgtaaggtt cgatgccgac cagatagccg cccttttcca ggtcggcgat
    13561 gacattgtcg cgcgcctcgt agcggtcctg tcccttgtag gatccgccgt attcgttgat
    13621 gatcccctct ccgtcgatga cggagatgat ctccaggttg tggcgctgcg ccatggcgaa
    13681 gtcgttgggg tcggaaccgg gagtgatctt caccgctccg gagccgaaat ccatcgtcac
    13741 gtagtcgtcg gcgataatcg gaatgggctt gttcatcaga ggcaaaatga ccattttgcc
    13801 gatcttgtcc ctgtagcgcg gatcatcggg gtgcacggcc accgccgtgt cgcccagcat
    13861 ggtttccggc cgcgtcgtgg cgacgacgat ttcgcctgag ccgtcggcaa acgggtagcg
    13921 gatattccac agattgctct ggtcggcctt gaattccact tccagatcgg aaatggcgg
//
LOCUS       DAOPAF010000037        13883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4417_length_13883_cov_56.636607, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000037 DAOPAF010000000
VERSION     DAOPAF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13883
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_13883_cov_56.636607"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLQ77_05130"
     CDS             <1..882
                     /locus_tag="PLQ77_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL10092.1"
                     /translation="AGIVRIGLDRGSMDKIIAENRQNIFIFMLFVAGIALLSMWLLYH
                     DQNRHLAGIIEMERRLEKAERLSSLGQLAAGVAHEIRNPLNAISMATQRLKRDYVPAD
                     KLKAHEFNNLSTVIRDEIRRLNAIIEEFLSFSKSRRLELRDFSVTEVLQKIVSLLAEE
                     AGSRGIALETKWQLSPAVIPMDINKLQQAFLNFIKNAMESISKKGVITITVVREGKNN
                     ILVSITDTGCGMTSEEIEKIFSPEYTTKEKGLGLGIPLATEIVRGHGGDVRVVSRLGE
                     GTTFEIVLPIKKKCEKT"
     gene            900..2261
                     /locus_tag="PLQ77_05135"
     CDS             900..2261
                     /locus_tag="PLQ77_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPL10093.1"
                     /translation="MRKLNILIVEDGQSQREMLRDFLISEGHAVTEAENGEAAVSAVS
                     NRHFDLLLLDYKMPGMSGLEVLEEVKKINHEIDVVIITAYGTIETAVEAIKAGAVDYI
                     TKPIELDELLILVDRVAERRGLIRENDLLKQELGKTGVTAEKIIYKSSRMVELINLAG
                     RVAASRASVLIQGESGTGKELLARLIHQLSPRASRPIVVVNCGALQENLLESELFGHE
                     KGAYTGADARRIGRFEEADGGTLFLDEIGELSPAIQVKLLRFLQEREISRLGSNASIS
                     VDVRIISATNQDLDARVSDGAFREDLFYRLNVVAVSLPPLRERKEDLTALIDHYLEKF
                     TRENGKNIQGLTAEARDLLLKYDYPGNIRELVNIMERAVVIAREDYITVGDLPFKNES
                     FNNFSGRNISGSLLEMREELEKNLIIEAMAKEDNNQTRAAASLGMTRRMLRYKLKKYD
                     LKN"
     gene            complement(2301..3044)
                     /locus_tag="PLQ77_05140"
     CDS             complement(2301..3044)
                     /locus_tag="PLQ77_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL10094.1"
                     /translation="MNKILRILYQPYKWLIFAPYLAVSTLFFGSLTVVLAVVTNPRIT
                     SFICGTIWARLNGYLTPIRVKVTGRENIDQTQSYVIVANHQSQYDIFVLYGWLGIDFK
                     WVMKQELRKVPGIGIGCEKVGHIFIDRSNHEKALASLRAAKEKIVNGTSVIFFPEGTR
                     SRDGSLGVFKKGAFKMAVDLRLPILPITIVGTRDILPTDSVDLFPGRARMIIHKPIDT
                     GGYKDDNLDELVDRARGIIGSGLWKGTPE"
     gene            complement(3131..3613)
                     /locus_tag="PLQ77_05145"
     CDS             complement(3131..3613)
                     /locus_tag="PLQ77_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPL10095.1"
                     /translation="MQVFAASDIVEVAIRIEENGINFYKFAEQIARQEDAKKLFSQLA
                     QAEAAHKRTFEKFFADMEKYNPPEQYAGEYSAYLRNYVDNNLIFTKEIMDRQLAKVTD
                     TVGAFEFAMQRELDSVLYYHEIKNLVPAAQHEAIDKIIEEERRHYTMLCDMKKRYENR
                     "
     gene            complement(3694..4755)
                     /gene="corA"
                     /locus_tag="PLQ77_05150"
     CDS             complement(3694..4755)
                     /gene="corA"
                     /locus_tag="PLQ77_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPL10096.1"
                     /translation="MTIAVKKRSKKAGLSPGALIHIGRDYEDSSGIRLMRYDEATLEE
                     KEIFSPTELAAEKKKPGVLWVHVDGLKDIRRLENIGRVFGLHPLILEDILSTDQRPKA
                     EDFTDYIFIVLKNIQPDAGPALHAEQISILLGQNFVLSFQEKAGTLFDPIRERIRADK
                     GRIRKAGADYLAHHLMDAVVDQYFAVLEDIDDKIELLEDDLVRKTTPDKLQSIHLLKR
                     ELILLRKSLWPLREAIGLLQRADSPLIADSDLIYFKDISDHIIAVIDTVDTFRDMLAG
                     MLDIYLSSASMRMNQVMKVLTIIATIFMPLTFLAGIYGMNFAYMPELGWRWGYFAVLG
                     LMLTIALGMLMLFRRKKWI"
     gene            complement(4768..6219)
                     /locus_tag="PLQ77_05155"
     CDS             complement(4768..6219)
                     /locus_tag="PLQ77_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPL10097.1"
                     /translation="MDLISTAASVVKNIRVQDILDMAIIAGMIFALLMWFKTRASRFV
                     LIGVLLLGGVYLAARFFQLYLTVIVLQGFFAILLFVLVVIFQEDLRGFFERIAMLGQL
                     RKQAPPLSRLEKTANIIAETAGNLAKKRVGALMVIQGNDPLDRHLTGGIPLNGAASEP
                     LLESLFDPHSPGHDGAVILKEDRVTLFGCHLPLSVNTARPGNMGLRHTAALGLTERSD
                     ALCVVVSEERGSISVSEGESLSVIPGASTLHEVLEKYYAKYRPAPQKRPLRAWLSENT
                     KEKLIAVGLAFVLWATFGYQRDTVRRDFYVPLEYKNMPARWHFSEPPVKEVKVTLQGP
                     EQAFRLMNADSLKLSLDLSALAQKKLEFSLNRDMVNAPPGLSIAEISPSKIRIYAARQ
                     VAAHLPVHVATVNEPPDDLALQKISVRPAALSVMLDPKINPGGLRLETETLDLQNITS
                     TGTFTLKVKPLSGAFFPDGKSPQVRVTVRVRKK"
     gene            complement(6408..6845)
                     /locus_tag="PLQ77_05160"
     CDS             complement(6408..6845)
                     /locus_tag="PLQ77_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPL10098.1"
                     /translation="MNYVRDILKTKDGRIQTISPKATVYEALEKMSEKEIGALVVMEN
                     KKVVGIFSERDYARKIILQGKTSKKTLVKEAMSSHLFSVTPDTTVEDAMVLMTGKHIR
                     HLPVFEKSKFVGIISIGDVLKLIISNKDFLINQLSDYIAGKYY"
     gene            complement(6961..7719)
                     /locus_tag="PLQ77_05165"
     CDS             complement(6961..7719)
                     /locus_tag="PLQ77_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HPL10099.1"
                     /translation="MKTQATPPKYQQTTGQDFSSGDLIVEAGLLFLGAPYRENTLDGP
                     GRERLIVDLRAFDCTTFVETVLALVRSASSGKFRAAEFRRNLQRIRYRGGKIDGYASR
                     LHYFTDWLADNQKKKVLRDVSRILGGVHARKKIDFMTAHRERYASLKRESQAKKMRQT
                     ERRLSRRPFYMIGKERASQIQNGIQPGDIIAFTTSQEGLDVAHVGFAIRERGRLRLLH
                     ASSREGAVAITAGTLPAYLKSRSNFTGILVARMI"
     gene            complement(7767..9152)
                     /gene="thrC"
                     /locus_tag="PLQ77_05170"
     CDS             complement(7767..9152)
                     /gene="thrC"
                     /locus_tag="PLQ77_05170"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPL10100.1"
                     /translation="MPIRYYSTNRRVQQASGITPFAESVSFREALLAGQAPDEGLFVP
                     DAIPQLSMKDIIALRDRPYWEAALLVARAFLSDEFSPEVLDAIVKDAYNFPVPLEAVY
                     PRAFILRLDQGPTASFKDFAARMMARLMSHCRPEGERLNILVATSGDTGSAVGEAYKG
                     VDGIDVTILYPADEVSLMQKKQLDTIGGNVRAMCVDGKFDDCQNLVKEAFADKDLARF
                     NLSSANSINFGRILPQIVYYVYAYAQLAGAGEEAVVSVPSGNFGNALGCEYARRMGLP
                     VAKLIMPTNENDEFPAFLETGIYTKVSPSRACISNAMNVGHPSNLARFFELYGGTVDR
                     TGMVHRYPDLEEMRRRIFSVSISDGQTRQTIMSVYERYRVVLEQHGAVGWAGLAAYQK
                     QTGDTRLAVCLETAHPAKFPDEIKNLLGIDPEPPESMKGLAGREGSAINLSRDFASFK
                     TYLRTHLKIKK"
     gene            9355..10407
                     /gene="selD"
                     /locus_tag="PLQ77_05175"
     CDS             9355..10407
                     /gene="selD"
                     /locus_tag="PLQ77_05175"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:9406..9408,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPL10101.1"
                     /translation="MEEKKTVRLTETVQSAGUACKIGPGDLSDILSDLPLISDPKLVT
                     GYEHAEDAGVYKMSDDLALVQTVDFFTPTVDDPFTFGQIAAVNAMNDIYAMGGTPLTA
                     MNIVCFPVKTMDKSVLRDVLRGGLDKMREAGVLLVGGHSVEDNEIKYGLAVTGTIHPA
                     RVLFNRGARAGDKLILTKPLGTGIVSTAIKAGAAGENLIARATACMTTLNKKAAELMI
                     SAGDIHSCTDITGFGFLGHACETIDGSDVGLRIEAARIPILEGVRDLSETGYLPGGLY
                     RNRNYRLHQVEKAPSCPDWIFDVCFDPQTAGGLLFSLPSEKAEPLAEAMRRAGITDAA
                     VAGDVVADHPGKILLE"
     gene            10414..11478
                     /locus_tag="PLQ77_05180"
     CDS             10414..11478
                     /locus_tag="PLQ77_05180"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPL10102.1"
                     /translation="MHRLPFKMETQQNVKKLLELAHSDDSLLLLMYGSPDPDAIASAM
                     ALREILLRTKGLSRWVFASTEPIRRQQNRELAQAMRLNIRPLAQVDAGAYRLVALVDA
                     QPSFFGDSPPTRAPNIVMDHHPREGDWQAELADIRPSYGALSTILTEYLMAARVRIPR
                     NLHTALLYGIKTDTDNFDRETIAEDIRAYTYHSRHANLRLIRRIELNQTPHSYLKYFD
                     YAFHSMTHFRGRRVGFLGRVESPDVCVQVADFYLHVIGTSYVVVAGIVDDRLVIIFRG
                     DGYRQDCGAMAQQACGQIGQGGGHRSAARLEIPLDVLKTELKEDLSRQSVERFLHQCL
                     RKSHLPKEDPPAAGSGREKT"
     gene            11475..12512
                     /locus_tag="PLQ77_05185"
     CDS             11475..12512
                     /locus_tag="PLQ77_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10103.1"
                     /translation="MKDIGFHRETVTGRYLNPFDGFEPREMTIEERYDETTGVASRIL
                     PYRVRPAQKPDTGVYLEKSPESICPFCPELFDKMTPRFTPDIIEGGKFTRGSAFLFPN
                     AFPYDATNTVAIFSHRHFMGLDELTPDAMHDGFAVCRDYFYRMAELQQGYHYGSINWN
                     YMPPAGGGLIHPHLQTIMGKEPTSFMRRLLASARNYASASGGRNLWEDLLDREQEAGE
                     RFIASTGVVSWLAAFSPKGMAGEVDFYFREKTSFFDLTQANVEELLEGLSRIFLHLYV
                     NHFMSFNLSLYAPLMPEKNFWVQGKIVPRFEINPLGTSDINYFEKLHDEIICPIVPEQ
                     LCKELQGYFQT"
     gene            complement(12606..12890)
                     /locus_tag="PLQ77_05190"
     CDS             complement(12606..12890)
                     /locus_tag="PLQ77_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPL10104.1"
                     /translation="MPIYEYQCRKCGRQFEAFQGITEPDLKTCRFCRGKVQKMMSLSS
                     FSLKGTGWYATDYKGKKPQAAKPKSTETTATESSSASSDASATVKGTSDD"
     gene            complement(12908..13444)
                     /locus_tag="PLQ77_05195"
     CDS             complement(12908..13444)
                     /locus_tag="PLQ77_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10105.1"
                     /translation="MIRYFNAAHIPVAARAFSDAEKLVTRHFRLTEDDLRKNKYDVKT
                     LAFLEEHEVNDGAFAHLCKYSYEKSSDGKPQEKEGFEFYRVCLQDHIILDALERANTF
                     IKLSPLMLYIAVHELIHVLRFSSGAADFSAPADEKENEEKIVHSLTRSALQPARQTDM
                     NMVLDCFSSEYKISNLYN"
     gene            13627..>13883
                     /locus_tag="PLQ77_05200"
     CDS             13627..>13883
                     /locus_tag="PLQ77_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10106.1"
                     /translation="MAQTGNCRNKNENDENTVWGMAVLRPFALIGRNVLSWVDNLGAA
                     AIFLGMALWQIFRRKQFAKTVRQVYYIGARSITIIMLVSLFT"
BASE COUNT         3016 a         3780 c         3957 g         3130 t
ORIGIN      
        1 gccgggatcg tccgcatcgg tctggaccgc ggttccatgg ataaaatcat tgccgaaaac
       61 cgccagaata tttttatttt catgttgttt gtcgccggaa tcgctctttt gtccatgtgg
      121 cttctctacc acgaccagaa ccggcatctg gccgggatta tcgaaatgga acgaaggctg
      181 gaaaaggcgg agcggctttc ttccctgggg cagctcgccg cgggggtggc ccacgaaatc
      241 cgcaatccgc tgaacgccat cagcatggcc acgcagcgcc tgaaaaggga ttatgtcccg
      301 gccgataagc tcaaggcgca tgaattcaat aatctttcca ccgtcatccg cgatgaaatc
      361 cggcggctca acgccattat cgaagaattt ctttcctttt ccaaaagccg ccgcctcgag
      421 ctgagggatt tttccgtaac ggaggttctg caaaaaattg tcagtttgct tgcggaggag
      481 gccggctcac gggggattgc gctggaaacg aaatggcagt tgagtccggc tgtcattccc
      541 atggatatca acaaactgca gcaggcattc ctgaatttca tcaagaacgc catggagtcg
      601 atttcaaaga aaggcgtcat cacgattacc gtcgtcaggg aaggcaaaaa taatatcttg
      661 gtcagcatca cggacaccgg ctgcgggatg acgtcggaag aaatcgaaaa aatattcagt
      721 ccggagtaca ccaccaagga aaaaggactg ggtttgggca ttccactggc cacggagatt
      781 gtccgcggcc acggcggcga tgttcgcgtt gtcagccgcc taggtgaggg gacgacgttt
      841 gaaattgtgc tgccgatcaa aaaaaagtgt gaaaaaacct gacgccgggg acggaaaaaa
      901 tgcgcaaatt gaatattctg attgtggaag acggacaatc gcagcgcgaa atgcttcggg
      961 attttctcat ttccgaagga catgcggtca cggaagcgga aaatggcgaa gcggccgttt
     1021 cagcggtttc gaaccgtcat tttgatttgc ttctgctgga ctataaaatg ccgggcatga
     1081 gcggactgga agtcctcgag gaagtcaaaa aaatcaatca tgaaatcgat gtcgtgatta
     1141 ttaccgccta cgggaccatt gaaaccgccg tggaggcgat caaggcgggg gccgtggatt
     1201 acatcaccaa gccgatcgaa ctggatgaac tgctgattct cgtggaccgg gtggcggagc
     1261 ggcgcggtct gatccgtgaa aatgaccttc tgaagcagga gctgggaaaa accggcgtca
     1321 ccgcggaaaa aataatttat aaaagttccc gaatggtgga actgatcaac ctggccggcc
     1381 gcgtcgcggc cagccgcgca tccgtgctga tccaggggga aagcggcacc ggcaaggaac
     1441 tgctggccag gctgattcat cagttgagcc cgcgcgcttc caggcccatt gtcgtggtga
     1501 attgcggcgc cctgcaggaa aatctgctgg agagcgagct gttcggccat gaaaagggcg
     1561 cttacaccgg cgccgacgcc aggcgcatcg gccgctttga agaagcggac ggcggcactc
     1621 tttttctgga tgaaatcggc gaactgagcc ccgccattca ggtcaaatta ttaagatttc
     1681 tgcaggagcg ggagatttca cggctcggca gcaatgccag catttccgta gatgttcgaa
     1741 tcatcagcgc caccaatcag gatctggatg cccgtgtcag tgacggagct tttcgggaag
     1801 atctgtttta ccggctgaat gtcgttgccg tgtccctgcc tccgcttcgc gaaagaaaag
     1861 aagatttgac ggcgctgatc gatcattatc tggagaaatt tacgcgggaa aacggcaaaa
     1921 acattcaggg gctgaccgcg gaggccagag atttgctctt gaaatacgac tatccgggca
     1981 atattcggga actggtcaat attatggagc gcgcggtggt catcgcacgg gaagactata
     2041 taaccgtagg cgatctgcct ttcaaaaatg aatcctttaa taatttttca ggcaggaaca
     2101 tctccggatc tcttttggaa atgcgggaag aactggaaaa aaatctgatt attgaggcaa
     2161 tggccaagga agacaacaac cagacgagag ccgccgcaag ccttggaatg acgcggagaa
     2221 tgctgcgcta caagctgaag aaatacgatt tgaagaattg agagggcccg cgggccgcgc
     2281 cggccgacgg atgcagagtg ttattcgggc gtccctttcc aaagccccga tccgataatg
     2341 ccgcgcgccc ggtccaccag ttcgtccagg ttgtcatcct tgtagccgcc ggtgtcgatg
     2401 ggcttgtgga tgatcatgcg cgcccgcccg ggaaagagat cgacggagtc cgtgggcaga
     2461 atatcgcgcg tgccgacgat ggtgatgggc aggatcggca ggcggagatc cacggccatc
     2521 ttgaaggccc cttttttaaa gacgcccagc gagccgtccc tgcttcgggt gccctccggg
     2581 aaaaagatca ccgacgtgcc gttgacgatc ttttctttcg ccgcccgcag cgaggcaagg
     2641 gccttttcat gattggaccg gtcgatgaaa atgtgcccga ctttttcgca gccgatcccg
     2701 atgccgggca cttttctcaa ctcctgcttc atcacccatt tgaaatcaat tcccagccag
     2761 ccgtacagga caaagatgtc atactggctc tggtggttcg cgacgatgac gtaggactgc
     2821 gtttggtcga tgttctctct tcccgtgacc ttcacccgga tgggcgtgag atagccgttg
     2881 aggcgggccc agatcgttcc gcagatgaag ctggtgatcc ggggattcgt gacaacggcc
     2941 agcaccaccg tcagagatcc gaaaaacagt gtggaaacag ccagatacgg agcaaagatc
     3001 aaccatttgt agggttgata caaaatacgc agtattttat tcaccgtttt ctttccaaat
     3061 caaaattcgg agcatcagtt gatcgaacgg ctgaataggg cctgggtccg aacccgtacg
     3121 ggcgttttcg ctatcggttt tcgtagcgtt ttttcatgtc gcacagcatg gtgtagtgcc
     3181 gtctttcctc ttcaatgatt ttgtcgatgg cttcatgctg ggccgccgga accaggtttt
     3241 tgatttcatg gtagtacagg accgaatcca gttcgcgctg catggcaaac tcaaaggcgc
     3301 ccaccgtgtc cgtcactttt gcgagctgcc ggtccatgat ttccttggta aagatcaggt
     3361 tgttgtccac gtagttgcgc agataggcgc tgtactctcc ggcatactgc tccggcggat
     3421 tgtatttttc catgtcggcg aaaaactttt caaacgtgcg tttgtgcgcg gcctccgcct
     3481 gcgcgagctg ggaaaagagc tttttggcgt cttcctgcct ggcgatctgt tcggcgaatt
     3541 tataaaaatt gattccattc tcctcaattc gaatcgccac ttcgacgatg tcacttgctg
     3601 cgaacacctg cataggggtt tcctcctgat agaatataat tacgcctgaa aactgaacat
     3661 attttgacgg ctattgcaac ctttttggct gtttcaaatc cattttttcc tgcggaacaa
     3721 cataagcatt cccagggcaa tcgtcaacat gaggcccagg acggcaaaat aaccccaccg
     3781 ccagcccagc tccggcatgt aggcgaaatt cattccgtag atgcccgcca gaaacgtcag
     3841 cggcatgaaa atggtcgcga taatggtgag caccttcatg acctggttca tccgcatgct
     3901 ggcggaggac aggtagatgt ccagcatgcc ggccagcatg tcccggaagg tgtccaccgt
     3961 gtcaatgacg gcgatgatgt gatcggaaat gtccttgaaa taaatcagat cggaatcggc
     4021 gatgagcggg gaatcagccc gctgcagcag accgatggcc tcccgaagcg gccagagcga
     4081 cttgcgcagc aaaatcagct ctcttttcag caggtggatc gactgcagtt tgtcgggcgt
     4141 ggttttcctg acgaggtcat cttccaggag ttcaattttg tcgtcgatgt cctccagcac
     4201 ggcaaaatac tgatcgacga ctgcgtccat caggtgatgc gccaggtaat ccgcccctgc
     4261 cttgcggatt cgtcccttgt cggcgcggat tctttcacgg atgggatcga acagcgttcc
     4321 cgctttttcc tgaaaggaca aaacgaaatt ctggcccagc aggatgctga tctgctccgc
     4381 gtgcagggcc gggccggcgt caggctggat gttttttaaa acgatgaaaa tataatccgt
     4441 gaagtcttcc gccttcggcc gctggtcggt gctgagaatg tcttccagga tcaggggatg
     4501 aaggccgaac acgcggccga tgttctccag acgccgtata tccttcaggc cgtcgacgtg
     4561 gacccagagg acgcccggtt ttttcttttc cgcggccagt tccgtaggcg agaagatttc
     4621 cttctcctcc agcgtggctt cgtcgtagcg catcagccgg atgccggatg aatcctcata
     4681 gtcgcggccg atatggatca gggctccggg gctcaacccg gcttttttcg atctcttttt
     4741 cacggcgatg gtcatggcgc tggctcctta ttttttcctg acgcggacgg tgacccggac
     4801 ctgcggggat tttccgtccg ggaaaaaagc gccggacaac ggcttgactt tcagcgtgaa
     4861 agtcccggtg gaagttatgt tttgcagatc gagggtctcc gtttccagcc gaaggccgcc
     4921 gggattgatc ttggggtcca gcatcacgct cagcgcggcc ggccgcacgg atattttctg
     4981 cagggccagg tcatccggcg gctcgttgac ggtcgcaacg tggaccggta gatgcgcagc
     5041 cacctgccgg gccgcgtaaa tccggatttt ggaggggctg atctcggcaa tcgacaaacc
     5101 cggcggagcg ttcaccatgt cccggttcag ggaaaattcc agttttttct gagccagcgc
     5161 cgacagatcc agcgaaagct tcagcgaatc tgcattcatc agccggaagg cctgttcggg
     5221 tccctgcagc gttaccttga cctccttcac cggaggttcg ctgaaatgcc atctggccgg
     5281 catatttttg tattcgaggg gaacgtaaaa gtcacggcgc accgtgtccc gctgatagcc
     5341 gaacgtggcc cacaagacaa aggccaggcc gacggcaatc aatttttcct ttgtgttttc
     5401 gctgagccag gcccgaagag gtcttttttg cggcgcgggc cggtacttcg cgtagtattt
     5461 ttccagaacc tcgtggagcg tcgaggcgcc ggggatgacg gaaagcgact ccccctcgga
     5521 gacggagatg ctgccgcgct cttcggacac gacaacacac agggcatccg atcgttccgt
     5581 gagccccagc gcggccgtgt ggcgcagccc catgtttccg ggccgcgcgg tattgacgga
     5641 caggggcaga tgacagccga acagggttac gcggtcttcc ttgaggatga ccgccccgtc
     5701 gtggcccgga gaatgaggat cgaaaaggct ctccagaagc ggctcgctcg cggcgccgtt
     5761 taagggaatg ccgccggtca aatggcggtc cagcggatcg tttccctgga tgaccatcag
     5821 ggcgccgacg cgctttttgg cgagattccc cgccgtctcc gcgatgatgt tcgcggtttt
     5881 ttccagcctg gacaggggcg gcgcctgttt gcgaagctgg cccagcatgg cgatgcgctc
     5941 aaaaaagccg cgcaggtctt cctggaaaat caccaccagc acaaacagca aaatggcgaa
     6001 aaatccctga agcacaatca cggtgagata aagctgaaaa aaccgggcgg ccaggtaaac
     6061 gccgcccaac agcaaaacgc cgatcaggac aaagcgcgac gccctggttt tgaaccacat
     6121 cagcagggcg aaaatcatcc cggcgatgat cgccatgtcc agaatgtcct gaacccgtat
     6181 gtttttgacg acggacgcgg cggtgctgat caaatccatg gcggcacgtt tccagttcga
     6241 gaaaaatgac tggctgtgta tagcggcatt tgatgacgga tgtcaaggat caaggcaaaa
     6301 aaacgcctcc ccgaaaaggg gaggcgaatg ggcggggcaa accgcgagaa actccttttc
     6361 ccgaagcgtc gtcggcaaat gccgtcttcg ggaaaagggc ttcgccttta gtagtatttt
     6421 ccggcgatgt agtcgctgag ctgattgatg aggaaatcct tgttggagat gatcagcttc
     6481 aggacgtcgc cgatggaaat gatgccgaca aatttggatt tttcaaaaac gggcaaatgg
     6541 cggatatgct ttcccgtcat cagaaccatc gcgtcttcca cggtggtgtc cggggtgacg
     6601 gagaacaaat gggaagacat ggcttccttg accagggtct tcttggatgt ttttccctga
     6661 aggatgattt ttctggcata atctctttcc gaaaagatgc ccaccacctt cttgttttcc
     6721 atgaccacca gagcgccgat ttccttctcg ctcatttttt ccagggcttc atatacggtt
     6781 gccttgggag aaatcgtctg aatcctgccg tccttcgtct tgagaatatc cctgacatag
     6841 ttcatagtcg cctcctgatt ttccttgttt ggtttgtctt catcttaata tatgtgcgct
     6901 caaaaataaa ggtgaaaatg cacgataaaa cagggcggcg cgtgatgatt ttcccgcttg
     6961 ctagatcatt cgggcgacca gaatgccggt gaaattactt cggctcttca gataagccgg
     7021 aagcgttccg gcggttattg ccacggcgcc ttcccggctg gaggcgtgca gcaggcgcag
     7081 gcggccccgc tcacggatgg caaacccgac atgcgccacg tccagccctt cctggcttgt
     7141 cgtgaaggcg atgatgtctc ccggctgaat gccgttctgg atttgggatg cccgttcttt
     7201 gccgatcatg taaaacggcc tgcgcgagag cctgcgttcc gtctgccgca tttttttcgc
     7261 ctggctttcg cgcttcagcg aggcgtagcg ttcgcggtgg gcggtcatga aatcgatttt
     7321 tttgcgcgca tgtacgccgc ccagaatgcg cgaaacgtcc cgaagcacct tttttttctg
     7381 attgtcggca agccagtccg taaagtagtg aaggcgggaa gcgtagccgt cgatttttcc
     7441 cccgcggtag cgtatgcgct gaaggtttct tcggaattcc gccgcgcgaa actttccgga
     7501 cgaagcggac cggaccagcg ccagcacggt ttccacaaag gtcgtgcagt caaacgcgcg
     7561 aagatcgacg atgagccttt cacggcccgg cccatcgagc gtgttttccc ggtaaggcgc
     7621 gcccagaaat aagaggccgg cttcaacaat caggtctccg gaagaaaaat cctgtcccgt
     7681 cgtctgttgg tattttggag gtgttgcctg tgttttcatc cgttgccaca cggtttgccg
     7741 gggtccgccg gtcggccgcc cggtttttat tttttgattt tcagatgcgt ccgcaggtac
     7801 gttttgaagg aggcaaaatc ccgggacagg ttgatggcgc ttccctccct ccccgccagg
     7861 cccttcatgc tttccggcgg ttcgggatcg atgcccagca ggtttttgat ttcgtcgggg
     7921 aactttgccg gatgggcggt ttccagacag acggcgagcc ttgtgtcgcc ggtttgtttc
     7981 tggtaggccg caagccccgc ccagccgacc gcaccatgct gctccagcac cacgcggtag
     8041 cgctcataaa cgctcatgat ggtttgccgg gtctggccgt cgctgatgct cacggaaaaa
     8101 atgcgccgcc gcatttcctc caggtcggga tagcggtgaa ccattcccgt gcggtccacg
     8161 gtgccgccgt acaattcaaa gaatcgcgcc aggttgctgg gatgccccac gttcatggcg
     8221 ttggaaatgc aggcgcgcga cggcgacact ttcgtgtaga ttccggtttc cagaaaagcg
     8281 ggaaactcgt cgttttcatt ggtgggcatg atcagttttg ccaccggcaa tcccatgcgg
     8341 cgggcgtatt cgcagcccag ggcgtttccg aaattgcctg aagggacgga gaccaccgct
     8401 tcctcgccgg cgccggccag ttgggcgtag gcgtagacgt agtaaacgat ctgcggcaga
     8461 atgcgcccga agttgatcga gttggccgac gacagattaa accttgccag gtctttgtcg
     8521 gcaaacgctt ctttgaccag attctggcag tcgtcgaact ttccgtcaac gcacatggcc
     8581 cgcacgttgc cgccgatggt gtcgagctgt tttttctgca tcaggctcac ttcgtccgcc
     8641 gggtacagga tggttacgtc gatgccgtca acgcccttgt aggcttctcc gacggcgctt
     8701 cccgtgtcgc cggacgtcgc caccagaatg ttgagccttt ccccttcagg ccggcagtgg
     8761 ctcatgagcc gggccatcat tcgggcggcg aaatccttga aggacgccgt ggggccctgg
     8821 tccagccgaa gaatgaaggc gcggggatac accgcttcca gcgggacggg gaaattatac
     8881 gcatccttca cgattgcatc gaggacttcg ggtgaaaact catcggagag aaacgcccgg
     8941 gcgaccagca gcgccgcctc ccaatagggc cggtcgcgaa gcgcaataat atccttcatg
     9001 gaaagctgcg gaatggcgtc gggaacaaac aggccctcat ccggcgcctg tccggccagc
     9061 aacgcttccc ggaaagatac gctttcggca aagggcgtaa tgccggaagc ctgttgaacg
     9121 cggcgattgg tgctgtagta gcgaatgggc atgcccggta tcctttgtga actggcttcc
     9181 tataatctga atgacgccaa taaatcaaca aaaaacaatc ctctctgtcc gccgtccttc
     9241 aatgccccgc cggggcggac gtgaaaagcg tctgaattcc gtttggaaaa tcggcgcttc
     9301 tatgttaaaa aaccaaacgc cgtgaggcgc aaattctttt tggattggaa catcatggaa
     9361 gaaaaaaaga ccgttcgtct gacggaaacc gtccaaagcg cgggttgagc ctgcaagatc
     9421 ggtccggggg acctgtccga cattctcagc gatttgccgc tcatttccga tccgaaactg
     9481 gtgaccgggt atgaacatgc ggaagacgcg ggcgtgtata aaatgtccga cgacctggcg
     9541 ctggtgcaga ccgtggattt cttcaccccg acggtggatg accccttcac cttcggccag
     9601 atcgctgcgg tcaatgccat gaacgacatt tacgcgatgg gcggcactcc tctcaccgcc
     9661 atgaacatcg tctgttttcc cgtaaagacc atggacaaat cggtgctgcg cgacgttttg
     9721 cgcggagggc tggacaagat gcgcgaagcc ggcgtgctcc tggtcggcgg ccacagcgtg
     9781 gaagacaacg aaatcaaata cggactggcg gtgaccggca cgattcatcc ggcccgcgtg
     9841 ctgttcaacc gcggcgcgcg tgccggcgac aagctcatcc tgaccaagcc gctgggcacg
     9901 ggcattgtat cgacggccat caaggcgggg gcggccggcg agaacctgat tgcccgggcg
     9961 acggcctgca tgacgacgct caacaaaaaa gccgccgagc tgatgatctc cgcgggagac
    10021 attcattcct gcacggatat caccggattc ggtttcctgg ggcacgcctg cgaaacgatt
    10081 gatggaagcg acgtcggcct gcggatcgag gcggcccgaa ttccgattct ggagggcgtg
    10141 cgggatctat cggaaaccgg ctatcttccc ggcggtcttt accgcaaccg gaactaccgc
    10201 cttcatcagg tggaaaaggc gccgtcctgt ccggactgga tttttgacgt ctgcttcgat
    10261 ccgcagacgg cgggcggcct gctgttcagc ctgccttccg agaaggccga gccgctcgcg
    10321 gaggccatgc gccgcgcggg catcacggat gcggccgtcg caggcgacgt cgtggctgat
    10381 catccgggga aaatactctt ggagtaatgc ctcttgcatc gtcttccctt caaaatggaa
    10441 acgcagcaga atgtcaaaaa gcttcttgag cttgcgcatt cggacgattc gctgctgttg
    10501 ctgatgtacg gcagcccgga tccggacgca atcgcttcgg ccatggcgct tcgcgaaatc
    10561 cttctgcgga ccaagggcct ctccagatgg gttttcgctt cgacggagcc gatccggcgg
    10621 cagcagaacc gggaactggc ccaggcgatg cgcctcaata tccggccgct tgcccaggtt
    10681 gatgccggcg cctaccggct cgtcgcgctg gtggacgcgc agccttcttt ttttggagat
    10741 tccccgccga cccgcgcgcc gaacatcgtc atggatcacc atccccgcga gggagactgg
    10801 caggccgaac ttgccgacat tcggccttcg tacggcgcgc tctcgacgat tttaacggaa
    10861 tacctgatgg ccgcgcgggt gcgcattccc cgcaaccttc acaccgcgct gctttacggc
    10921 atcaagacgg acacggacaa tttcgaccgc gaaacgattg ccgaagacat tcgcgcctac
    10981 acctatcatt cccggcacgc caacctgcgg ctgatccgcc ggatcgaact caatcagacg
    11041 ccgcacagct atttgaaata tttcgattac gcgtttcatt ccatgacgca tttccgcggc
    11101 cgccgcgtcg gtttcctggg acgcgtggaa agtccggatg tctgcgtgca ggtggcggat
    11161 ttctacctgc acgtgatcgg cacgtcgtac gtggtggttg cgggaatcgt ggatgaccgg
    11221 ctggtcatta ttttccgggg ggacggctac cggcaggact gcggcgcgat ggcgcagcaa
    11281 gcctgcggcc agatcggaca gggaggcgga caccgcagcg ccgcgcgcct ggaaatcccc
    11341 ctggacgttc tcaaaacgga gctgaaagag gatttgtccc gacagagcgt ggagcgtttt
    11401 cttcatcaat gcctgaggaa aagccatttg ccgaaagaag atccgcctgc ggccggaagt
    11461 gggagggaaa aaacatgaaa gacatcggat ttcacaggga aacggtcacc ggacggtacc
    11521 tcaatccctt tgacgggttt gaacccaggg aaatgaccat tgaggagcgc tacgacgaaa
    11581 ccaccggcgt ggccagccgc attcttcctt accgtgtgcg gcccgcccag aaaccggaca
    11641 caggcgtcta tctggaaaaa tcgccggaat cgatctgccc gttttgcccg gagctctttg
    11701 acaagatgac gccccggttc acgccggaca tcatcgaagg cggcaaattc acgagaggaa
    11761 gcgcttttct gtttcccaac gcgtttccct acgacgcgac aaacacggtt gcgatctttt
    11821 cgcaccgaca cttcatgggg ctggatgaat tgacccccga cgccatgcac gacggatttg
    11881 ccgtctgccg ggattatttt taccggatgg ccgaactgca gcaagggtac cattacggtt
    11941 cgatcaactg gaactacatg ccgcccgcgg gcggcggact gattcatccc cacctgcaga
    12001 cgatcatggg caaagagccg accagtttca tgcggcggct tctggccagc gcgcggaatt
    12061 acgcctccgc ctccggcggc cgcaatctgt gggaagacct gctggatcgg gagcaggaag
    12121 caggcgagcg ttttattgcc tcgaccggcg tcgtgtcttg gcttgccgct ttttcgccaa
    12181 agggcatggc gggggaagtt gatttttact ttcgggagaa aacctcgttt tttgatctga
    12241 cccaggccaa cgttgaagag cttctcgaag gtctttcccg gatctttctc cacctgtatg
    12301 tcaatcattt catgagcttc aatctttcgc tgtatgcgcc cctgatgccg gagaaaaact
    12361 tctgggtgca ggggaaaatc gttccgcgtt ttgaaatcaa tccgctgggg acgagcgaca
    12421 tcaattattt tgaaaaactg cacgatgaga tcatctgtcc gattgtgccg gagcagttgt
    12481 gcaaagaatt acaggggtat ttccagacat agaggaggga acggtttcaa accgttcttc
    12541 gcaaaaaaaa caaaaaagcg gcattttcat gccgcttttt gttggccttt tttaagtgaa
    12601 tgcttttaat cgtcgcttgt gcctttgacg gttgccgaag catcggatga agccgaagaa
    12661 ctttccgtcg cagtcgtctc agtgcttttc ggcttggcgg cctgaggttt ttttcccttg
    12721 tagtccgtcg cgtaccagcc ggtgcctttc aggctgaagg atgaaagcga catcattttc
    12781 tgaaccttcc ccctgcagaa ccggcaggtt ttcaaatccg gctcggtaat gccctgaaac
    12841 gcttcaaact gccggccgca tttcctgcat tgatattcat agatcggcat acatcaacct
    12901 cctgaaatta attataaaga ttgctgattt tatattcgct gctgaagcag tccaaaacca
    12961 tgttcatatc cgtttgcctg gccggctgca gcgcgcttcg ggtcaggctg tggacgattt
    13021 tttcctcatt ttccttttcg tccgcggggg cgctgaaatc agcggcgccc gaggagaagc
    13081 gcaggacgtg gatcaattca tggacggcaa tatacagcat caaaggactc aatttgatga
    13141 aggtattggc gcgctccagt gcgtccagga tgatatggtc ctgcaggcac acccggtaaa
    13201 actcgaaacc ttccttctcc tgcggcttgc cgtcggaaga cttttcgtag gaatatttgc
    13261 acaggtgtgc aaatgcgccg tcatttactt catgctcttc caaaaatgca agggttttta
    13321 catcgtattt gtttttccgc aggtcatctt ccgtcaggcg gaaatgacgc gtcaccagct
    13381 tttcagcgtc ggaaaacgcc ctggccgcca ccggtatatg cgccgcgtta aaatagcgaa
    13441 tcataaaaac accttaaatg ccgccgcccg atttggccgt agtatattca ttcttcgtgt
    13501 ttctgtcaac acggacggga acacccggac gggaaaccgg cgtcaggaaa tgcttgtcaa
    13561 ataaagttgc ctctgttagt atcccgaacc gtcttcgaca ggcgatttga tctggggaac
    13621 ttgttcatgg cgcaaacggg aaattgccgg aataaaaatg aaaatgacga aaacaccgtc
    13681 tggggcatgg ctgttcttcg cccgtttgct ttgatcggaa gaaacgtttt gagctgggtg
    13741 gacaacctcg gggcggcggc catttttctg gggatggcgc tgtggcagat tttccgcagg
    13801 aagcagtttg ccaagacggt ccggcaggtt tattatatcg gggccagatc catcacgatc
    13861 atcatgctgg tcagcctgtt tac
//
LOCUS       DAOPAF010000038        13638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4518_length_13638_cov_36.222705, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000038 DAOPAF010000000
VERSION     DAOPAF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_13638_cov_36.222705"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLQ77_05205"
     CDS             <1..383
                     /locus_tag="PLQ77_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HPL10107.1"
                     /translation="DALKFMDFVFLHLEAPDEMGHEGNAKGKIMAIECFDEKIVGPIL
                     TRIGELGEYRILVLSDHPTPIDVKTHVGDPSPFAVLSSKREENLARGLKFTEAQARDA
                     GLIVSPGHRLMDGFLQDWRAFLGK"
     gene            373..792
                     /locus_tag="PLQ77_05210"
     CDS             373..792
                     /locus_tag="PLQ77_05210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPL10108.1"
                     /translation="MESNLTTFRVIYADTDAMGIVYHTNYIRWFELGRNELMRQMGIV
                     YSDLENLGLHLPLTTVFCRYLQPARYDDLLTVETCFDYIKRASIRYSSKIWDENQRKL
                     LVEGYTIHACTSNEGKIRRMPALITGLIEKYSIRKGE"
     gene            794..1474
                     /locus_tag="PLQ77_05215"
     CDS             794..1474
                     /locus_tag="PLQ77_05215"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL10109.1"
                     /translation="MAAKLSKKELKSPDAFQTTFDRVGEYVEENKSRVIIAAAALSCA
                     VLLVLGIYFYWSYYSGSALKLYAKAQDNILKGGDNPETEKANIPILQKLADQYPHSWS
                     GRMAHYHLANIYYNQGDLDRAIASYETFVAKTGSDKTGVRFLALTSLGYAYEARKDFR
                     AALKYFEQAQKGYHTGFEMMSLRNVARAHEALNDKAKAMEYYQKALEKTTDSAAQLYL
                     RRKIAALG"
     gene            1527..3293
                     /gene="iorA"
                     /locus_tag="PLQ77_05220"
     CDS             1527..3293
                     /gene="iorA"
                     /locus_tag="PLQ77_05220"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPL10110.1"
                     /translation="MKKELMSGNEAIARGVYEAGVRFAAAYPGTPSTEIMEQFARYDG
                     VYAEWAPNEKVAVEVAIGAALGGAKALAVMKHVGVNVAADPIFTVSYTGIEGALVIIS
                     ADDPSMHSSQNEQDNRNYAKFAKIPMLEPSDPQEAKEYVKLAFAISEQFDTPVFLRTT
                     TRVSHSKSVVTLSEPRVPEDKTALAHHAAKYVMVPINARIRRVEVEKRQKALQKFAET
                     FPENKMEINDPAVGIITAGMPYNYAKDVFPDYSYLKLGMVYPLPVKMIRHFASRVKKI
                     YVIEELDPYLEEQIRALGIEVTGKEIFPYTNEFDPGIIRSAIHADKPAAASTYPETLA
                     QRPPNLCPGCPHRGLFYALKKSKAYVHGDIGCYTLSYLKPLEGLHSCICMGASIGMAH
                     GMSKALKEKGKGKVVGVIGDSTFLHSGITPLMNMAYNNSDALIIILDNSTTAMTGMQE
                     HPGTGYTLMGKEAKKVNLNILISALGIENIRVIDPNNVKEARSAIKDELASSGPSVII
                     AQSPCVLFKRANQKSKPPLKVDPDACEGCRSCLELNCPPIAWKKGVTKKGSKRKGIAV
                     IDETLCNGCGLCAQVCRFGAIS"
     gene            3310..3891
                     /locus_tag="PLQ77_05225"
     CDS             3310..3891
                     /locus_tag="PLQ77_05225"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPL10111.1"
                     /translation="MKEDRVKSILFAGVGGQGIIRASDILCEVMMEAGYDVKKSEVHG
                     MAQRGGCVTSHVRYGRKVYSPLAEPGTVETLVSFEKMEALRYLKDLSPNASIILNTEQ
                     INPPAVNTGDADYPLDIIEYLKMHYPQVMAIEALLLAQRAGNLKAANVVLLGALSSLM
                     DIPHAIWESVIRKSFPQKLVKLNLEAFQMGIPA"
     gene            3931..4887
                     /locus_tag="PLQ77_05230"
     CDS             3931..4887
                     /locus_tag="PLQ77_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPL10112.1"
                     /translation="MAEDYYKVLGVEKKATPEEIKKAYRKLAVKWHPDKNPNNKAAAE
                     EKFKKISEAYAVLSDAEKRQNYDNFGSADQFRQQYSQEDIFRGFDLDEILRSFGFGGP
                     RGGGRTTFRTGTRRGGYTEYDDPLAGIFGQMGGGGGQYANMPQKGQDAEYNLSISLEE
                     SVLGADKKISLQMEKGAENINVKIPPGISSGKKLRLPGKGLPGYNGGQNGDLYLNINV
                     LSHPIFARDGNDLYIEKTIKFSQAALGTTIDVPTIDGTMKRLKIAPGTQNNTKIRMKG
                     FGVPVLKESRNGDQFVKVNVEVPKKLNDKQARLIEQLAADGM"
     gene            complement(4937..5377)
                     /locus_tag="PLQ77_05235"
     CDS             complement(4937..5377)
                     /locus_tag="PLQ77_05235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPL10113.1"
                     /translation="MPEIQQYLRKKEGNHYMDDFTKELWKKSPFIKLLGLEFEKLEQG
                     RTSTRLERRENLLNKHSAVHGGVIYAIADISMGVAVYTTLKTGEETSTIEIKINYLKP
                     AKCNLLICEARVLKKGKNLAVVEAEIRSGESDLIAKALGTFMLT"
     gene            complement(5370..6128)
                     /locus_tag="PLQ77_05240"
     CDS             complement(5370..6128)
                     /locus_tag="PLQ77_05240"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPL10114.1"
                     /translation="MRFWGRGVRFIADMIHEERTYRSLINKDNLISYRVKISQSDLWI
                     SSDSNLASVAEKSLLKHRFSLESYIDRHPEFQTALLPYPQDFLAPGIVREMIARSAVC
                     GVGPMASVAGAIAEFVGNDLSGLTDHLIIENGGDIFLLSKSSLTIGIFAGESTLSYKV
                     KIIVKPEETPLGVCTSSATVGPSLSFGKADAVCVLSSSPTLADAAASAIGNRVNTRYD
                     IRNAMNCGMKIPGVKGIVIIFGNEMGAMGEVELA"
     gene            complement(6091..9564)
                     /gene="dnaE"
                     /locus_tag="PLQ77_05245"
     CDS             complement(6091..9564)
                     /gene="dnaE"
                     /locus_tag="PLQ77_05245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPL10115.1"
                     /translation="MKQADFVHLHVHTQYSLLDGMIRLDDLFQKAKEYEMPAIAITDH
                     GTMFGAIDFYKQACAAGIKPIIGCELYVAPRGLADRTPSPAGETARHLVVWAKDMQGY
                     KNLMKLTSVAHLDGFYYRPRVDKALLKECSQGLIASSACLHGEIASHIVRGRMEDARR
                     AAREFQEIFGEDNFYLEVMENGIPEQKTANRGLMELGRELSIPLIATNDCHYLNADHA
                     EAHDVLLCIQTGRTIEDKDRMSMSSGQFYVRSSGEMQALFAEMPEAVSNTMDVANRCN
                     LTLEFGKFYLPKYEIKDPGESLEDHLDRKATQGLEKLFPVILKHWKEDEAAVRQKYTD
                     RLRSELEIIKKMGFAGYFLIVSDFIKHAKHTGVPVGPGRGSAAGSLVAYAIRITDIDP
                     LLYGLFFERFLNPDRISMPDIDIDFCQERRGEIIKYVTEKYGKDRVSQICTFGKMMAK
                     GVIRDVGRALNIPYGEVDRIAKLVPTFPLNIKLADAFKAEPRFEEERRKNPKIDKMLS
                     LSLVLEGLNRHSSVHAAGIVISDVPLVERVPLFSPKKDDRVSQYSMKDIEAVGLTKFD
                     FLGLKTLTVIKNAVDFIRDSHHVDVDVNNLPLDDEETYRLLAKGETDGVFQLESAGMK
                     DLLVNFKPDRIEDVITLIAAYRPGPMKMIPDFIARKHGKQQIAYELPQLEPILKETYG
                     IILYQEQVMQIANVIGGYTMAQADTLRKVMGKKQVAAMEKEKPRFLEGAKKKTINENK
                     AKIIWNQMEDFAEYGFNKSHSAAYAMITYQTAYLKAHYPVAFMAALLTSEKDNRDKII
                     RHMSNCKEMGINILPPDINESQKDFSISGGNIRFGLAAVKNVGEAAIESVMEIRMEGK
                     FTSFMDFLCRIDLRKVNRRVLESLIKCGSFDSLHFTRRQLMECLDSAMEEAQRKQKEL
                     QSNQASIFDELAPARTSGTGGNGSFSIPELPEWDRKELLSIEKDTVGFYISGHPLQGY
                     AGKLHLVANTDSSSLNTKRDKETITVAGIISSLSERLTKKKEVMCNVVLEDLQGSVNI
                     IFWADIYRKFSDLLHADEPVVIQGMVDIGDESNKIIALDVIPLAKALENPYKQVRFMV
                     KTDKISPEGISALCETIRKYDGKYEGYIHLLNGKCETIVRLGDRTKLNICEKLRREAD
                     AILGEGSTVYC"
     gene            complement(9569..11029)
                     /gene="guaB"
                     /locus_tag="PLQ77_05250"
     CDS             complement(9569..11029)
                     /gene="guaB"
                     /locus_tag="PLQ77_05250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPL10116.1"
                     /translation="MLDDVVRESLTFDDLLLVPAASSILPRDVETSTWLTQRIKLNIP
                     IVSAAMDTVTESRTAICMAQEGGIGIIHRNMSIERQAIEVDRVKKSESGMVVDPITIE
                     PERKVSDALALMHQYSISGVPVVKNEKLVGILTNRDLRFEENLDQPVSNVMTKKNLIT
                     VKSSITLEESKKLLHKHRIEKLLVVDDENRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREPRIEALLAAGVDVIAIDTSHGHSAGVIDAIRSTKANFPKCELIAGNI
                     ATVEGAQALIDAGVDAVKVGVGPGSICTTRIIAGVGVAQMSAIRDTYKVASRQGIPVI
                     ADGGIKYSGDIVKALGCGANAVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYQDDVEAPSKTVPEGIEGRVPFRGTLSASIVQLIGGLKAGMGYVGCRTIA
                     ELIEKSRFVRITSAGLRESHVHDVIITKEAPNYWID"
     gene            complement(11019..11507)
                     /locus_tag="PLQ77_05255"
     CDS             complement(11019..11507)
                     /locus_tag="PLQ77_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HPL10117.1"
                     /translation="MNENDIRIDKDGIWYYRGAHMFRKDILCIFFEHLKRDDSGRYFI
                     ELNGEICGLEVEDTAFVVTAVCKIQDACGSETLEILLSDDRKEALDAGTLAVGKDNVL
                     YCRVKNNRFPARFTRKSYYQLAEFIEPDGDENEFCVVFDGRKYPIALASHNHDNGGNH
                     VR"
     gene            complement(11510..11959)
                     /gene="dtd"
                     /locus_tag="PLQ77_05260"
     CDS             complement(11510..11959)
                     /gene="dtd"
                     /locus_tag="PLQ77_05260"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPL10118.1"
                     /translation="MRAVLQRVDRAGVNINSRLFSSISQGLLVLLGVEKGDSRKDADY
                     VLEKTVNLRIFEDEQGKMNRSLADIGGEMMVVSQFTLLADCVKGRRPSFAGAEEPEKA
                     NELYEYFVSEASMKVKKLATGRFQEMMQIELINNGPVTILLDSRKTV"
     gene            12110..13498
                     /locus_tag="PLQ77_05265"
     CDS             12110..13498
                     /locus_tag="PLQ77_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL10119.1"
                     /translation="MSKKVLLINPWIYDFAAYDFWLKPLGLLYLGGLLRANHHRISCI
                     DCLDPYNSLMLQKSGKAPKRHAYGHGRFFRQVIEKPSCLEAIDRSYCRYGMDPSVFRK
                     EIARFSDTDVVLVTSMMTYWYPGVFDVIKILKEMLPGVPVILGGKYASLCHDHAAANS
                     GADFAVRGRGEKQILELMRSVFGEDIVFNPEEDDLDALPYPAFDLLGRIDQVPIITSL
                     GCPYRCSYCSSHLFYDRHMKRNPGKVADEIEYWHRRFGVMDFSFYDDALLADPESLII
                     PLLHDIQRRNLSCRFHCPNGLHVREIDAPLSRLLFESGFKTIRFGFETANFEAQRETG
                     GKVRNEELKRAVAHLKEAGYKTRDIGVYLLCGLPGQQAREVEESIDFVVRCGARPVLA
                     EYSPIPGTPMWPEAVAASIFDIQREPLYHNNTLLCCRNNDFSYEAYRGMKERIQALPK
                     ENGFGNGTSDSN"
BASE COUNT         3122 a         3673 c         3622 g         3221 t
ORIGIN      
        1 tcgacgcgct gaagttcatg gattttgtgt ttcttcacct ggaagcgccc gacgaaatgg
       61 gccatgaagg caacgcgaaa ggcaagatca tggccatcga gtgtttcgac gaaaaaatcg
      121 tcggaccgat tctgacgcgc atcggcgaac tgggggaata ccgtattctg gtgctcagcg
      181 atcatccgac gccgatcgac gtgaaaaccc acgtgggcga tccgagcccc tttgcggtcc
      241 tgtcatccaa acgggaagaa aaccttgccc ggggactgaa attcaccgaa gcgcaggccc
      301 gcgacgccgg tctgatcgtg tctcccgggc atcggctgat ggacggcttc cttcaggact
      361 ggagagcatt ccttggaaag taacctgacg acattccgcg tgatctacgc cgacacggac
      421 gcgatgggca ttgtctatca caccaactat atccgctggt ttgagctggg ccggaatgaa
      481 ttgatgcggc aaatggggat tgtctattcg gatctggaaa acctggggct ccatctgcca
      541 ttgacgacgg tgttctgccg ctatctccag ccggccagat acgacgacct gctcacggtg
      601 gagacttgct ttgactatat caagagggcc agcatccgtt acagctccaa gatctgggac
      661 gaaaatcagc gaaaactgct cgtggaagga tacaccatcc atgcctgcac cagcaacgaa
      721 ggaaaaatcc ggaggatgcc ggcattgatc accgggttaa tcgagaaata ttctattcgc
      781 aaaggggagt aaaatggccg ccaagttatc caagaaagag ctgaaaagcc cggacgcttt
      841 tcaaaccacc tttgacagag tgggcgagta cgtcgaggaa aataaaagcc gcgtgatcat
      901 cgccgccgcg gccctgtcgt gcgccgtgct gctggtcctg ggcatttatt tttactggag
      961 ctattattcc ggttccgcgc tgaaattata cgccaaagca caggacaata tcctgaaggg
     1021 cggcgacaat ccggaaacgg aaaaggccaa catcccgatc ctgcagaagc tggcggatca
     1081 gtacccccac agctggagcg gccggatggc tcattaccat cttgccaata tttactacaa
     1141 ccagggagac ctggaccggg cgatcgcttc ctacgaaaca ttcgtcgcca aaacaggatc
     1201 ggataaaacg ggcgtgagat ttctggcgct gacgtctctg ggctacgctt atgaagccag
     1261 gaaggatttc agggcggctc tcaaatattt tgagcaggcg caaaaaggtt accataccgg
     1321 ctttgaaatg atgagtctgc gcaacgtggc gcgcgctcac gaagcgctga acgacaaggc
     1381 caaggcaatg gagtactacc aaaaggctct ggaaaaaaca acggattcgg cggcccaact
     1441 ctatcttcga agaaaaatag ccgccctggg ctgagttccc gcggcgggtc ccggaccgcc
     1501 atcggttatg gaggaggcgc aagcagttga aaaaagaatt gatgtctgga aacgaagcca
     1561 tcgcccgcgg cgtgtacgag gcgggcgtca ggtttgcggc tgcctatccg ggcacgccca
     1621 gcaccgaaat catggagcag tttgcccggt acgacggtgt ttacgccgaa tgggcgccca
     1681 atgaaaaagt cgcggtggag gtggccatcg gcgcggccct gggcggcgcg aaagccctgg
     1741 cggtcatgaa gcacgtcggc gtgaacgtgg ccgccgatcc gatttttaca gtaagctaca
     1801 ccggaatcga gggcgcactg gtcatcatca gcgccgatga cccgtccatg cacagctcgc
     1861 aaaacgagca ggacaaccga aactacgcca agttcgccaa aattcccatg ctggagccgt
     1921 ccgatcccca ggaggccaag gagtatgtca agcttgcctt cgccatcagc gaacaattcg
     1981 acactccggt ttttctgcgc acgactacgc gcgtctccca ttccaagtcc gtggtcacgc
     2041 tcagcgagcc gcgggttccc gaagacaaaa ccgcgcttgc gcatcatgcg gcaaaatacg
     2101 tcatggttcc catcaacgcg cgcatccgcc gcgtggaagt ggaaaagcgc cagaaagccc
     2161 tgcagaagtt tgcggagacg tttccggaaa ataaaatgga aatcaacgat ccggcggtcg
     2221 gcatcattac cgcgggcatg ccatacaact acgcgaagga cgtttttccg gattattcct
     2281 atctgaagct gggcatggtc tatccgctgc cggtgaaaat gatccggcat ttcgcctcca
     2341 gggtcaaaaa gatttatgtc atcgaggagc tcgatcccta tctggaagag cagatcaggg
     2401 ccctgggcat cgaggtgacc ggcaaggaga tcttccccta cacgaatgaa tttgaccccg
     2461 gcatcattcg aagcgcgatc catgccgata agccggccgc cgcttcgact tatccggaaa
     2521 ccctggcgca acggccgccg aacctctgtc ccggatgccc gcaccgggga ttgttttacg
     2581 cgctgaaaaa atccaaagct tacgtccacg gcgatatcgg ctgctacacg ctgtcctatc
     2641 tgaagcccct ggaagggctg cattcctgca tctgcatggg cgccagcatc ggcatggccc
     2701 acgggatgag caaggccctt aaggaaaaag gaaaggggaa ggtggtcggc gtgatcggcg
     2761 attccacctt tcttcactcc ggcatcacgc ccctcatgaa catggcctac aacaacagcg
     2821 atgccctgat catcattctg gacaacagca cgacggccat gaccggcatg caggagcatc
     2881 cgggaacggg ctacaccctg atggggaagg aagccaagaa agtcaacctg aatattctga
     2941 tatcagccct gggaattgaa aatattcgag tgatcgatcc gaacaacgtg aaggaagccc
     3001 gcagtgcgat caaggatgaa ctggcgagct ccggcccttc cgttattatc gcccagagcc
     3061 cctgcgtttt gttcaaaagg gcaaaccaga agtcgaagcc gccgctgaag gtggatccgg
     3121 acgcatgcga aggctgcagg tcctgcctgg agctgaactg tccgccgatc gcctggaaaa
     3181 aaggcgtcac gaagaagggc tccaagcgaa agggaatcgc ggtgattgac gagacgctgt
     3241 gcaacgggtg cgggctttgc gcacaggtct gcagattcgg cgcgatcagc tgaagaaaag
     3301 gaaagtttta tgaaagaaga cagggtaaaa agcattctgt ttgccggagt gggcgggcag
     3361 ggcatcattc gggccagcga tatcctctgc gaagtcatga tggaagccgg ctacgatgtc
     3421 aagaaaagcg aagtccacgg catggcgcag cgcggcggct gtgtcaccag ccacgttcgt
     3481 tacggcagaa aggtttattc gcccctcgcg gagccgggaa ccgttgaaac gctggtttcc
     3541 tttgagaaaa tggaagccct gcgctatctg aaagacttaa gcccgaatgc ttccatcatt
     3601 ctgaataccg agcagatcaa tcccccggcc gtcaatacgg gggatgctga ttatccattg
     3661 gatatcattg aatatctaaa aatgcattac cctcaggtaa tggcgattga ggcacttttg
     3721 ctcgcccaga gggccggcaa tctgaaagcg gccaatgtcg ttcttttggg agctctttcc
     3781 tcactgatgg acattccgca tgcaatctgg gaaagcgtta ttcggaaatc ctttccgcaa
     3841 aaacttgtaa aattgaatct tgaagctttt caaatgggaa ttcctgctta aattaccgcc
     3901 ataagaaaag aaaaataaga ggaaatttgc atggctgaag attactataa ggttctgggc
     3961 gttgaaaaaa aggccacgcc ggaggaaatc aaaaaagcct accgaaagct ggccgtcaag
     4021 tggcatccgg ataaaaatcc caacaacaaa gcggcagccg aggagaaatt caaaaaaatc
     4081 agcgaggctt acgctgtttt aagtgatgcg gaaaagaggc agaattacga caatttcggc
     4141 tcggctgatc agtttcgcca gcaatattca caagaagaca tcttccgcgg gttcgacctc
     4201 gacgagattt tgcgcagttt cggcttcggc gggcccaggg gaggggggcg aacaaccttc
     4261 cgcacgggaa cgagacgggg cggctatacg gaatatgacg acccgctggc cggcattttc
     4321 ggtcaaatgg gcgggggcgg cggccagtat gccaatatgc cgcaaaaagg ccaggatgcc
     4381 gaatataacc tatcaatatc ccttgaagaa tcagttttgg gcgccgacaa gaaaatttcg
     4441 cttcagatgg aaaagggcgc tgaaaacatt aatgtcaaaa tacccccggg aatcagctcc
     4501 ggaaagaaac tgaggcttcc cggaaaaggc ctgccgggtt acaacggcgg gcaaaacggt
     4561 gatctctacc tgaatatcaa tgttttgtcc catccgatct tcgcccgcga cggcaatgac
     4621 ctgtacatcg aaaaaaccat caagttttcc caggccgctc tggggacaac catcgacgtt
     4681 cccacgatcg acggaaccat gaaaagactg aagatcgcgc ccggaacgca aaacaatacc
     4741 aagattcgaa tgaagggatt tggagtgcct gtcctgaagg aatcacgcaa tggcgatcag
     4801 ttcgtcaaag taaacgttga ggttccgaaa aaactcaacg ataagcaggc tcgattgatt
     4861 gaacaactgg cggcggatgg gatgtagcag cccgcccgcc gcattgccgc attttcttta
     4921 tgtcctataa gatatcttat gttaacatga atgtgccgag agcctttgcg atcagatcgc
     4981 tttctcccga ccttatctcc gcctccacga cggctaaatt ttttcctttt ttaagaactc
     5041 gcgcttcgca aattaaaaga ttgcatttcg ccggctttag atagttgatc ttgatttcaa
     5101 tcgtggaagt ttcttcacct gttttcaatg tggtgtaaac agccacgccc atgctgatat
     5161 cggctatggc atagataacg cctccgtgaa cggcgctgtg tttgttgagc aggttttctc
     5221 tcctttcaag acgggtggaa gtgcgcccct gttccagctt ttcaaattca agcccaagca
     5281 gtttgataaa cgggcttttc ttccatagtt cctttgtaaa atcatccata tagtgatttc
     5341 cttccttctt cctgagatat tgttgtattt caggcaagct cgacctcccc cattgcgccc
     5401 atttcatttc caaagataat gacaatgccc ttcacgcccg gtattttcat tccgcaattc
     5461 atggcatttc gaatatcgta tcgggtattt acgcgattgc cgattgcgga ggccgctgca
     5521 tccgccagcg tcggggaact ggaaagaacg cagaccgcgt cggcttttcc gaagctgaga
     5581 gacggcccga ccgttgcgga tgatgtacag acacccaaag gcgtttcttc aggttttaca
     5641 attattttaa ccttataact taaagtagat tctccggcaa agattccgat ggtcaggctg
     5701 cttttggaaa gaagaaaaat atcccctccg ttttcaatga tgagatgatc cgtcaaaccg
     5761 gaaagatcgt ttcccacaaa ttccgcgatt gcgcctgcaa cggaggccat tggccctacc
     5821 ccgcagacgg ctgatcgcgc aatcatctcc ctgacgattc ctggcgccag aaagtcctgt
     5881 ggataaggaa gcagcgccgt ttgaaattcc ggatgcctgt cgatatagga ttcaagggaa
     5941 aatcgatgct tcagaagaga tttttcggcc actgaggcca ggttggaatc agagcttatc
     6001 cacaggtcgc tttgagaaat tttaacacga tatgaaatca gattatcttt attaattaat
     6061 gatctataag ttctttcctc atgtatcata tcagcaataa accgtactcc cctcccccag
     6121 aatcgcatcc gcctcccgtc ggagtttctc acagatgttt agttttgtgc ggtccccgag
     6181 acggacgatg gtttcgcatt tcccgttcaa caggtgaata tagccttcgt atttcccatc
     6241 gtattttcga atcgtttcac aaagggcgga aattccctcc ggggatattt tgtcggtttt
     6301 gaccataaaa cgaacctgtt tatagggatt ttccagagcc ttggccaggg gaatcacatc
     6361 cagagcgatg attttattag attcatctcc gatatcgacc atcccctgaa taacaaccgg
     6421 ctcgtcggca tggagcaaat cggagaattt cctgtagatg tccgcccaga agatgatatt
     6481 gacggaaccc tgcaggtctt caagaacaac attgcacatc acttcttttt tcttggtcag
     6541 cctctcagag agcgagctga tgatccccgc gacggtgatg gtttctttgt ctcttttggt
     6601 gttcagcgat gaagaatccg tgttggcaac caggtgcaac ttgccggcat agccctgcaa
     6661 tggatgcccg gaaatataaa aaccgacggt gtctttttcg atggagagca gttccttgcg
     6721 gtcccattcg ggcagctcgg gaatcgagaa agacccgttg ccgcctgtcc cggatgttct
     6781 ggcgggagcg agctcgtcga agatgcttgc ctgattgctt tgcaattcct tctgcttgcg
     6841 ctgcgcctcc tccatggccg aatccaggca ttccatgagc tgacggcggg tgaagtgcag
     6901 ggaatcgaaa gatccgcact tgatcagact ttccaggacc cttcggttga ctttgcgcag
     6961 atcgatgcgg cagagaaaat ccatgaagga tgtaaatttt ccttccatgc ggatttccat
     7021 tacggattca atcgccgctt ccccgacatt ttttacggcg gcaaggccga aacggatgtt
     7081 gcctcccgaa atgctgaaat ccttttggga ttcattgatg tccggcggca gaatattgat
     7141 gcccatttcc ttgcagttgg acatgtgccg gataatcttg tcccggttgt ctttttcgct
     7201 ggtcaaaagc gccgccatga aggcgacggg ataatgggcc ttcagatagg ccgtctggta
     7261 ggtgatcatc gcgtaggcgg cgctgtgaga cttgttgaaa ccgtattcgg cgaagtcttc
     7321 catctgattc catatgattt ttgccttgtt ctcgttgatc gttttctttt tcgcgccttc
     7381 aagaaaacgg ggtttctcct tttccatcgc cgctacctgc tttttcccca tcactttgcg
     7441 cagcgtgtcg gcctgcgcca tggtatatcc tccaatgacg ttggcgatct gcatgacctg
     7501 ttcctggtaa aggatgatgc cgtaggtttc tttgaggatc ggctccagct ggggaagctc
     7561 ataggcaatc tgctgctttc catgcttgcg ggcgataaaa tcgggaatca tcttcatcgg
     7621 tcccggccgg taggcagcaa tcagcgtgat gacgtcttcg atgcggtccg gcttgaaatt
     7681 gaccagcagg tctttcatcc cggcgctttc cagctggaaa acaccgtcgg tttccccctt
     7741 tgcgagcaga cggtaggttt cttcatcatc gagaggaaga ttgttcacgt caacatcgac
     7801 atggtgggaa tcccggatga aatccacggc gttcttaatg accgtcagcg tcttgagacc
     7861 caaaaaatca aatttggtca gcccgacggc ttcgatatcc ttcatcgaat actgggaaac
     7921 cctgtcatct tttttgggag aaaaaagcgg aacacgctcg accagcggaa cgtcggatat
     7981 cacaatgccg gcggcgtgca cggaagaatg ccggttgagg ccttccagaa ccagcgacag
     8041 cgacagcatt ttgtcgatct tcgggttctt cctgcgttct tcctcaaaac gcggctccgc
     8101 cttgaacgca tccgctagct tgatgttcag cggaaaggtc ggcaccagtt tggcaattcg
     8161 gtccacttcg ccatagggaa tgttcagggc tctgcccaca tcccgaatca cgcccttggc
     8221 catcatcttg ccgaaggtgc agatctggga gactctgtcc ttgccgtatt tttccgtgac
     8281 gtatttgatg atttcaccgc ggcgttcctg gcagaaatcg atgtcgatgt ccggcatgct
     8341 gatccggtcc ggattgagaa aacgctcgaa aaacaatccg tagagcaagg gatcgatgtc
     8401 ggtgatgcga atggcatatg ccaccagcga tccggcagcg gagccgcgtc ccggaccgac
     8461 gggcaccccc gtgtgcttgg cgtgcttgat aaaatcggaa acgatcagaa aatatccggc
     8521 aaaccccatt tttttgatga tttccagctc tgagcgcagg cgatccgtgt atttttgccg
     8581 aacggccgcc tcgtcctctt tccagtgctt caaaatcacc gggaacagtt tttccaggcc
     8641 ctgggtcgcc ttgcgatcca gatgatcctc aaggctttct ccggggtcct tgatctcgta
     8701 tttcggcagg tagaatttcc cgaattccag ggtcaggttg catcgattcg cgacatccat
     8761 cgtattggag actgcttcgg gcatttcggc aaaaagcgcc tgcatttccc cggaggaacg
     8821 gacgtaaaac tggcccgacg acatggacat ccggtctttg tcttcgatgg ttcttcccgt
     8881 ctgaatgcac agcaaaacat cgtgcgcttc cgcgtgatct gcgttcagat agtgacagtc
     8941 gttcgtggcg atgagcggaa tggagagctc cctgccaagc tccatcaagc cccggttggc
     9001 ggttttctgc tcgggaatgc cgttttccat gacctccaga tagaaattgt cctcgccgaa
     9061 aatttcctga aactcacggg cggccctgcg ggcgtcttcc atgcggcccc ggacgatatg
     9121 cgaagcgatt tccccgtgga ggcatgcgct tgacgcgatc agaccttggg agcattcttt
     9181 cagcagggcc ttgtccacgc gcggccggta gtaaaatcca tccagatggg ccacggaagt
     9241 cagtttcatc agatttttgt agccctgcat gtctttggcc caaaccacca gatggcgcgc
     9301 cgtttcaccg gcgggcgacg gggttctgtc cgcgagcccg cggggcgcga cataaagttc
     9361 gcagccgatg atcggcttga tgccggccgc gcaggcctgc ttgtagaagt cgatcgcgcc
     9421 gaacatcgtg ccgtggtcgg tgatggcgat cgccggcatc tcgtattctt tggccttttg
     9481 aaagaggtcg tccaggcgga tcatgccgtc gagaaggctg tactgcgtat ggacgtggag
     9541 atggacgaaa tcggcttgct tcatttggct aatctatcca gtaattgggc gcttccttgg
     9601 tgatgatcac gtcgtgaacg tggctttccc tgaggccggc cgacgtaatg cggacgaacc
     9661 gggatttttc gatcagttcc gcaatggtcc ggcatccgac gtagcccatg ccggctttca
     9721 gcccgccgat cagttggacg atgctggcgg aaagcgtgcc gcggaaaggc accctgccct
     9781 cgatcccttc cggaaccgtc ttggaaggcg cttcgacgtc gtcctgatag tagcggtcgc
     9841 ggcttcccat tttcatggcc tccagcgatc ccatgccgcg atacaccttg taactgcgcc
     9901 cctggaacaa aatcgtttca cctgggcttt cttcggttcc ggcaaacagt ccgccgatca
     9961 tgacggcgtt tgcgccgcag cccagggcct tgacgatgtc gccggaatac ttgatgccgc
    10021 cgtcggcaat gacgggaatg ccctgacggg aggcgacttt ataggtgtct cggatcgcgg
    10081 acatctgcgc cacgcccacg cccgcgataa tgcgggtggt gcagatggag ccgggcccca
    10141 ctccgacctt taccgcatcc actccggcat cgatgagcgc ctgggccccc tcgaccgttg
    10201 caatgtttcc cgcgatgagc tcgcatttgg ggaaattcgc cttggtggag cggatggcgt
    10261 caatcacgcc cgccgaatgt ccgtgagagg tgtcaatggc aatcacgtcc acacccgccg
    10321 ccagaagcgc ctcgattctg ggctcccggt cgatgatgcc cacggccgcg ccgacgcgca
    10381 gccttccgag cgagtccttg caggcgttgg gatagcggcg gattttttca atgtccttga
    10441 tggtgatcag gcctttgagc cggttttcat cgtccacgac caaaagcttt tcgatgcggt
    10501 gtttgtggag cagctttttg gattcttcca gggtgatgct ggactttacg gtgatgagat
    10561 tttttttggt catcacgttg gacacgggct gatccaggtt ttcttcaaag cgcagatcgc
    10621 ggttggtcag aatgccgacc agtttttcat ttttgacgac cggcacgccg gagatggaat
    10681 actggtgcat cagggccagc gcgtcgctga cttttctttc cggttcgatg gtgatcggat
    10741 cgaccaccat gccgctttcc gattttttca cccggtccac ttcgatggcc tggcgttcga
    10801 tgctcatatt ccggtgaata atgccgatgc cgccctcctg agccatgcag atggccgtgc
    10861 gggattccgt caccgtgtcc atggccgccg agacgatggg aatgttcagt ttgattctct
    10921 gcgtcagcca cgtggatgtt tcgacgtcgc gcggcaaaat gctggaagcc gcgggcacca
    10981 agagcaggtc gtcaaacgtc agggactctc ttacaacatc atctaacatg gtttcctccg
    11041 ttatcatggt tatgggaagc aagcgcaatc ggatatttcc gaccgtcgaa aacgacgcaa
    11101 aattcgtttt catcgccgtc gggctcaatg aattccgcca gttgatagta gcttttccgg
    11161 gtaaagcgcg cggggaaccg gttgtttttc acgcggcaat acaggacgtt gtctttgccg
    11221 acggccagcg tgccggcgtc cagcgcctcc ttccggtcgt cgctcaacag gatttccagt
    11281 gtttcagaac cgcaggcgtc ctggattttg caaaccgccg ttacgacaaa ggccgtgtct
    11341 tcaacctcca gtccgcagat ttctccgttg agctcgatga aataccggcc gctgtcgtcc
    11401 cgcttcaggt gctcgaagaa aatgcacaaa atgtctttgc ggaacatgtg cgcgccgcgg
    11461 taataccaga tcccgtcttt gtcgatccga atgtcgtttt cgttcatggt caaaccgttt
    11521 tcctgctgtc gagcagaatc gtcaccggtc cgttgttgat cagttcgatt tgcatcattt
    11581 cctgaaacct gccggtggcc agcttcttca ccttcataga cgcctcggac acaaaatatt
    11641 catacagctc attggccttc tccggctctt ccgccccggc aaacgacggc cttcgcccct
    11701 tgacgcagtc ggccagcagt gtaaactgcg aaaccaccat catttcgccg ccaatgtcgg
    11761 caagcgagcg gttcatcttt ccctgttcat cctcaaatat tcgcaaattg actgtttttt
    11821 ccagaacata atccgcgtct ttccgggaat cgcctttttc cacgccaagc aataccagca
    11881 acccttgcga aatagaggaa aataatcgtg aatttatgtt gacgccggcc cggtcaactc
    11941 tttgaagtac tgcccgcatg aagcatccct taaaaagttt gggcatgtta gcaacaattg
    12001 cccgagtctt caagttcttt ctgaaaatcc tgctgctgat cacgaattgc ctctcgacat
    12061 atgcttcatg cactttgtaa attatgggct tgtatgctaa agacacccca tgagcaagaa
    12121 ggttctgtta atcaatccct ggatttatga ttttgcggct tatgatttct ggctgaaacc
    12181 cctggggctt ttatacctgg gcgggctgct gcgcgccaac catcatcgca tttcctgcat
    12241 tgattgcctt gatccttata attcgctcat gctgcaaaaa agcggcaaag cgcccaaacg
    12301 ccacgcttac gggcatggcc gcttcttccg gcaggtcatt gaaaagccgt cctgcctcga
    12361 ggcgattgac cgcagctact gccgatatgg aatggatccg tccgttttcc gaaaagagat
    12421 tgcacgcttc tccgatacgg atgtcgtgct ggtcacgtcc atgatgacct actggtatcc
    12481 cggggttttt gacgtgatta aaatactcaa ggagatgctg ccgggcgttc ccgtcatcct
    12541 gggaggaaag tatgcctccc tttgccatga tcatgcggcg gcaaattctg gggcggattt
    12601 cgctgttcgc ggacggggtg aaaagcagat tctcgagttg atgcgaagcg tttttggaga
    12661 agacatcgtc ttcaatcccg aagaagacga tctggatgcc ctgccctacc ctgcgtttga
    12721 cctgttgggc cggattgacc aggttcccat catcacttcg ctgggctgtc cctaccgctg
    12781 cagctactgt tcctcccatt tgttttacga ccggcatatg aagcgcaatc ccggaaaagt
    12841 cgcagacgag atcgaatact ggcatcggcg attcggcgtc atggatttca gcttctacga
    12901 cgacgccctt ctggccgatc ccgaaagcct gatcattcct ctgcttcacg acattcaaag
    12961 acgcaatcta tcctgccggt ttcactgccc gaacggcctg catgtccggg aaatcgacgc
    13021 gccgctcagc cgtctgctct ttgaatccgg cttcaaaacg attcgcttcg gctttgaaac
    13081 agccaatttc gaggcgcagc gggaaaccgg cggcaaggtg cgaaatgagg aactgaagcg
    13141 cgcggtcgcg catctgaaag aggcaggata caaaacacgg gacatcggag tttacctttt
    13201 gtgcggcctg ccgggccagc aagcccggga ggtggaagag agcatcgatt ttgtcgtccg
    13261 gtgcggcgca aggcccgtgc tggccgagta ctcgccgatt ccgggaaccc ccatgtggcc
    13321 ggaagccgtc gccgcttcca tatttgacat tcagcgcgag cctctgtatc ataacaacac
    13381 tctgttgtgc tgccgcaaca atgatttcag ttatgaggct tatcgcggga tgaaagagcg
    13441 cattcaagcg ctgccgaaag aaaacggttt tgggaatgga acttctgatt cgaattaaaa
    13501 acatcgtcat ttttctgttg tccggatttt ttctggtgtt cggcgtcctt ctcctgaaaa
    13561 gctcctttca actggccaat ccgctggaat tcgtgatgac gctgtttgcg gcctctttca
    13621 tcattttgtt ttgcatcg
//
LOCUS       DAOPAF010000039        13503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4567_length_13503_cov_51.259221, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000039 DAOPAF010000000
VERSION     DAOPAF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_13503_cov_51.259221"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLQ77_05270"
     CDS             complement(<1..271)
                     /locus_tag="PLQ77_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10120.1"
                     /translation="MKKTIGTIVAAAAVVLLTATFGFAEYAAAGAANFPYFQLGCLIV
                     GGLIMVSLKRKYDKMYTAEVVGAFALYTLLMALFTNPVIDAVKNIV"
     gene            537..872
                     /locus_tag="PLQ77_05275"
     CDS             537..872
                     /locus_tag="PLQ77_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPL10121.1"
                     /translation="MKNLFADIPESPEDEWMETLLRAPGFRVERIVSRGHCSPCGFWY
                     DQEENEWILLLQGSAGLRVEGQKEILVLRPGDSFHLDRRKKHRVEWTASGQNTVWLAV
                     YYTGSSNFE"
     gene            885..1514
                     /locus_tag="PLQ77_05280"
     CDS             885..1514
                     /locus_tag="PLQ77_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10122.1"
                     /translation="MQLNHPEATQQALGILRSGDPFQWYVIPLLVIVLYIYANEYEKG
                     NYRVIAGGLSLYMVHWFVEIVNALVQHFSGHALWTVPAGTAFEILVGVGIEISLMFSI
                     AGLVVCKLMPKDPAVKILGIPNRLFFAVVNAAFASILEIFLVRTPAFVWVYDWWGAFP
                     VFVTVYIPFFAAACYVHDWQPRGQKLFIGSLFLVNAAALVVFAGILGWI"
     gene            1533..2018
                     /locus_tag="PLQ77_05285"
     CDS             1533..2018
                     /locus_tag="PLQ77_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10123.1"
                     /translation="MKRFLKVVIAGGLVLAFAAGCASAGTMLKSEAVSAVPEKKFEKI
                     IRLDTPECLPLQPDAGFVFVEPGGKVITDFPRQIAAASLYSLDRYERLNRDRMGAFVI
                     KDRQGGVRGYYELLFEYRAFIWERGEEILLQISIPRGRRGTIADAGINAAGMGAISVG
                     R"
     gene            complement(2075..3487)
                     /locus_tag="PLQ77_05290"
     CDS             complement(2075..3487)
                     /locus_tag="PLQ77_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL10124.1"
                     /translation="MKRKAHRLDPFFRPGSVALFGSMQEGWFFGAAVIISDLLKWNYR
                     GKIYPVHPAARTVYGIPVFPDLAHVKDIPELAVIVTSFRHAPGILEQCGAKGVKAAVI
                     VSDGFSEAGPEGRIRQEKLLGIAKSHGIQVMGPNTVGVFSSESRFTTVPYDRGYEYQK
                     QGRLSIITQTGMYSPQAMAWHEYEAGINKVIDLGNMCDVDETDCLDYLGEDPSTDVIS
                     LYMEHSRRPRMLLDVLKKVSRKKPVLCLMPGGSPGAAQAMASHTGSLAGNADLYDALL
                     RQGGALRVEEYEDLRDCATPFLRYPLPRGNRLGIITFSGAIGIQAIDAAEAFGLSIGK
                     LSPESRRKLAAQSGTLGGHPIDIGPASATAGAGIFDLYQKCFDILAADENIDCIYLNA
                     YVSNGLNPAFYEEMLGRIGSFRAKPVVSWCYGPSQQLVCEFGKLAERHGIPFFPNSKK
                     AVRSLSYMARYARRKTSVSE"
     gene            complement(3691..3891)
                     /locus_tag="PLQ77_05295"
     CDS             complement(3691..3891)
                     /locus_tag="PLQ77_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPL10125.1"
                     /translation="MSEGKVKWFNERKGFGFIEQEGGSDIFVHFSAIQSSGFKTLTEG
                     QQVSFDVVQGKKGMEAENVRAL"
     gene            complement(4256..4726)
                     /locus_tag="PLQ77_05300"
     CDS             complement(4256..4726)
                     /locus_tag="PLQ77_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPL10126.1"
                     /translation="MKKHLRKILVMAIIAFIASACASQDRFIVHHTNGTVLDTKTNLM
                     WAARDNGSDVNWTGAKSYCENYAAGHYKDWRMPTSEELAGLYDARQTREAPCAGNFNI
                     HMTTELIQVTCFAAWSSETRSADASLYNFIYGRPDWYLQSHTYGMRALPVRNNK"
     gene            5051..5884
                     /locus_tag="PLQ77_05305"
     CDS             5051..5884
                     /locus_tag="PLQ77_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPL10127.1"
                     /translation="MMSENRPAKFAGASRYVLDDELAKIVNISMALEMPLLLKGEPGT
                     GKTMLAHAIAENLRMPLIVLNVKSSMKLVDALYQYDTLTRLNDSRFSDSGRDVSNIEE
                     YIRMGKIGQAFVSDRKVVLLIDEIDKADTDFQDDMLDILDQMQFDIMEIDRTVKAKSR
                     PVIVITSNAKKDLSDPFLGRCNFHHIAFPDREMMRRIIGVHFPDLNRDLTSACLDTFY
                     RLREVRGVEKKPATRELINWMRALQADPDFNVKSLRSAGIPFLGVLFKKSTDLYLAAH
                     Q"
     gene            5966..7159
                     /locus_tag="PLQ77_05310"
     CDS             5966..7159
                     /locus_tag="PLQ77_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10128.1"
                     /translation="MFVNFFYTLRDKGVPVTPTSFLRLQKALGMGLVASLDDFYSVAR
                     SLLVKSEKYFDIYDQAFSAAFRGMADVDPTDAELTEAVQAMLSEWLRDPGGMARALGL
                     SEEQVKQMTPDELVRHFLRRLKEQTEAHHGGSYWIGSRGTSPTGHSGDHPGGMRVGGQ
                     SRNKSAIKVAMERRYRDYSQSGPITASQMGEALKRLKHLQPAGPRDVVNIDKTIDSTM
                     RNAGEIEIIFDRRMADRLKVKLMIDNGGWSMDPYVEIVQALFHYAQFQFKELEIYYFH
                     NTIYSKIWQDPARSAKPVRVDELIRSDPETRLIIVGDASMAPYELNDAHGAIYLGQNE
                     ARPSMDYLKFLSRTFRHSVWLNPQPQDSWFYTWTVKQIAGIFPMFELSLDGLDKAVHR
                     LRSRN"
     gene            7172..7741
                     /locus_tag="PLQ77_05315"
     CDS             7172..7741
                     /locus_tag="PLQ77_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPL10129.1"
                     /translation="MRKQIALIMLLVFLALPALLFAQTQPPRVGAALPDLKIQKPADS
                     AGLKYLGLSGSGTFAVDQVRAQALIIQIFSLYCPYCQKDAPNMNRFFGLIEGNPRLRG
                     KIKILGIGAGNTQFEVNTFKKKYKVEFALVPDGDFKIHKIIGEVRTPYFIIVKLDGPK
                     KREVVYSRLGVHEDVEAFLAETVRLTGIQ"
     gene            7755..8345
                     /locus_tag="PLQ77_05320"
     CDS             7755..8345
                     /locus_tag="PLQ77_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPL10130.1"
                     /translation="MKRKIACALLFAALFAAPAFALSDAYKGNVFNTGRLKPVDSVLK
                     VKVGQAAPDFTLRAVSGKKVSLTDYRGKKNVVLSFVPAAWTPVCSDQWPGYNVVQELF
                     EENDAVLLGISVDNIPTLHSWTSQMGQLWFDVLSDFWPHGAAASRYGILRSDGTAERA
                     IIIIDRKGIIRYIDVHDINERPTLESIIGQLEKLRK"
     gene            8500..9129
                     /locus_tag="PLQ77_05325"
     CDS             8500..9129
                     /locus_tag="PLQ77_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPL10131.1"
                     /translation="MSSEERRKKEKENRKNSILKAARKLFFERGFKSVTVDLIAAKAE
                     VSKGSIYLYFDSKEEIYTQILISANIDRHKEWDVFSKREGTASEALIAFAHEYVSYFL
                     DNNELFRILMTFMLQAENMNLTDEQNTQLIHTTNENVHVIAEILQRGVESGEFIAGMD
                     VRRMQFAIWGLLNGVISLYIYTGAPERRGERIFTIVHDSLNALLKGIKS"
     gene            complement(9126..9488)
                     /locus_tag="PLQ77_05330"
     CDS             complement(9126..9488)
                     /locus_tag="PLQ77_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPL10132.1"
                     /translation="MEIKYKVWIEKNGKVVFGKGRDDILKAIDEQRSLNAAAKKLEMS
                     YRAAWGRLKASEERMGMKLVEIGVHDKSMQLTGQARSIIERFERLEKDVERLLHTADQ
                     DFQKLLQEEGKTGKTVGS"
     gene            9690..12758
                     /gene="fdnG"
                     /locus_tag="PLQ77_05335"
     CDS             9690..12758
                     /gene="fdnG"
                     /locus_tag="PLQ77_05335"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:10275..10277,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HPL10133.1"
                     /translation="MQITRRGFLTMTGAIGGGVALSSLGLNLNPTRAYADELGKMDRI
                     KIAKQVTTICCYCSVGCGLVCSVDKATGKIFNIEGDADHPINEGSLCAKGAGFFDLTE
                     ANKHRLTKVLYRKPYGTEWEEKDWDFALSRIARLVKDTRDKNFTKRNDKGELVNRCEA
                     IGHYGSSNVDNEECWIMSVKSRALGMVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIAFKWILRAKEKGATIIHVDPRYTRTSARCDFHVPLRS
                     GTDIAFLGGMIHYILENKKFFKDYVLNYTNASFIVDNGFNFKDGLFSGYDPKKRKYDK
                     ATWVFDKDGKGIPQRDMSLTHPRSVFQLLKKHYSRYTLDKVSSITGVSKENLLKVYEI
                     YASTGVPDKAGTECYALGWTHHTTGSQNIRTMSIIQLLLGNMGIAGGGINALRGEPNV
                     QGSTDHCILYGNLPGYLKMLSASLDTLDKYLHKYTPESKDPQSANYYSNYPKFFVSFL
                     KSLWEDKATKENAFGYSWLPKMDDGKHYSTMHMFDAMYAGKVKGYFCVGADTAVSTPN
                     SNKVRKAMENLDWLVGENIFDNETHSFWKGPGVDPKKIKTECFLLPAAATMEKEGSQS
                     NSGRWVQWKYKAAEAPGDALPVGEIEIKIMAAVKKLYEKEGGPNAEAIVNLKWDYLDS
                     KGHFDVIKVAKRINGVFLEDTVIEDKAKGTKTEFKKGQLVPGFGNLQADGKTACGNWC
                     ISGSYTADGINKMASRGKEDPTGLGLFPNWSFAWPVNRRILYNRASCDVNGRPYNPKR
                     KLLEWTGDKWAGDVPDGPWPPQADRAKGKYPFIMQRDGLGALFGPGMAEGPFPEHYEP
                     LEGPLEKNLLSSQRMNPAAEIFKSDMDQVANASEKFPYVCTTYSCTEHWCTGALTRWQ
                     AWLLEMQPELYVEIGEKLAREKGIKNGERIKVSSIRGEAPCIAMVTKRFKPFTVDGKE
                     VHQVGMPFNYGWLFPKDSGSDSTNMLTPTVGDANTFCPEYKAFMVNVEKL"
     gene            12772..>13503
                     /locus_tag="PLQ77_05340"
     CDS             12772..>13503
                     /locus_tag="PLQ77_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10134.1"
                     /translation="MNKQPELVKLIDTTLCTACRGCQVACKQWNELPGLKTKQLGTYQ
                     NPPDLQWNTWTLIRFQEYEDKKGNFQWIFRKDGCMHCTDAACVKVCPSGSLYYTPLKT
                     VGIHRDKCIGCKECVSACPFHIPKYDAKTDKVYKCDLCYSRIKESHAPACVKSCPTGA
                     LTIGDKDAMIKKAYARVKELGSDANVYGDKFVDGTHVIYVLQFKPEVYEELPVNPKVP
                     LSVIVWKDLLKPLSLLAAGGVLGGAF"
BASE COUNT         3096 a         3585 c         3797 g         3025 t
ORIGIN      
        1 tgacaatgtt tttcaccgcg tcaatgaccg gattggtgaa gagcgccatc agaagagtgt
       61 aaagagcgaa ggcgccgacg acttccgccg tgtacatctt gtcatacttc ctcttcaggc
      121 tgaccatgat cagtccgccc acaatgaggc agcccagctg aaaatacggg aaattggctg
      181 cccctgccgc tgcatactct gcaaatccaa aggttgccgt caaaagaacc actgccgccg
      241 ccgctacgat tgttccgatg gtcttcttca tgatctgttt cctccctgag ggtttttgtt
      301 tcatttgcct tatacttaat gaaaattttg tgccagaatt ggaaaacagg cgaggaatct
      361 ctgcaaatat atgatattag aaacaaatca ctggatggtg tcctggaaag gaaggctccc
      421 gaaacttcat ttcccgcttt ttcctgaacc aaatgttcaa tggggttgaa cgttatgatg
      481 aaaatcgggt ataagaagta caattttaaa gattgtatta gcgggagggc gaacgcgtga
      541 aaaatctttt tgccgacatt cccgaatcgc cggaagacga atggatggaa acgcttcttc
      601 gggcgcccgg ttttcgcgta gagagaattg tttcccgcgg ccattgctcg ccttgcggtt
      661 tctggtatga ccaggaggag aatgaatgga tcctcctcct gcagggaagc gccggcttgc
      721 gcgtggaagg gcaaaaggaa attctggttt tgcgtccggg agattccttt cacctggatc
      781 gccgcaagaa acaccgggtg gaatggacgg cttccgggca gaatacggta tggctggccg
      841 tctattacac cggttcatcc aacttcgaat aaaggaggca tgcgatgcaa ttgaaccacc
      901 ctgaggcaac ccagcaggcg ctcggcatct taagaagcgg cgaccctttt cagtggtatg
      961 tcattcctct tctggtgatt gtcctgtaca tctatgccaa tgagtatgaa aagggcaatt
     1021 accgcgtgat cgccggagga ctgtcccttt acatggtcca ctggtttgtg gaaatcgtca
     1081 acgccctcgt gcagcatttt tccggccacg ccctgtggac cgtgccggcg gggaccgcct
     1141 ttgaaattct ggtgggcgtc ggcatcgaaa tcagcctgat gttttcgatc gccgggcttg
     1201 tggtctgcaa actgatgccg aaagatccgg ccgtgaagat tctgggaatc ccgaatcgtc
     1261 tgttcttcgc cgtcgtcaat gcggcttttg cctccattct ggaaatcttc ctggtgagaa
     1321 ccccggcatt tgtctgggtt tacgactggt ggggcgcttt tccggttttc gtgacggttt
     1381 acattccctt ttttgccgcc gcctgctacg tgcatgactg gcagccgcgc gggcagaagc
     1441 tgttcatcgg ctcacttttc ctcgtgaatg ccgccgccct ggtcgtgttc gcgggaattc
     1501 tcgggtggat ctgagaaagt gaggatacaa aaatgaagcg gtttttgaag gttgtcatcg
     1561 cgggagggct tgtcctggcg ttcgccgccg gctgcgcgtc tgccggcacg atgctcaaaa
     1621 gcgaagcggt gagcgccgtg ccggaaaaga aatttgagaa gatcatacgg ctcgacacac
     1681 cggaatgcct tccgctgcag ccggatgccg gatttgtctt tgtggagccg gggggtaaag
     1741 tcattacgga ttttccgcgg cagatcgcag ccgccagcct ctattcgctg gaccgatacg
     1801 aacggctgaa ccgggatcgc atgggggcgt ttgtcatcaa agaccgccag ggcggtgtgc
     1861 gcggttacta tgaattgctg ttcgagtatc gggcgtttat ctgggagcgg ggcgaggaaa
     1921 ttctattgca gatctcgatt cccagaggca ggcggggcac catcgcggac gccggaataa
     1981 atgccgcggg catgggggcg atttccgtcg gccgctaaac cgcgcggctt gcggccgttc
     2041 gtccggggcg gaggaagaag cggcgccgaa gcggttattc ggaaacagat gttttccggc
     2101 gggcgtatcg ggccatgtag gaaagagacc ggacggcttt tttgctgttt gggaaaaagg
     2161 gaatgccgtg gcgttcggcg agttttccaa attcgcaaac cagttgctgc gacggaccgt
     2221 agcaccagct gacgaccggt tttgcccgga aggatccgat tcggccaagc atttcttcat
     2281 aaaaagccgg gttcaatccg ttggaaacat aggcgttgag gtaaatgcag tcgatgtttt
     2341 catccgcggc gagaatgtcg aagcattttt ggtacaggtc gaatattccc gcgcctgccg
     2401 tggcggaggc cggcccgatg tcgatggggt ggccgcccag cgtaccgctt tgggcggcaa
     2461 gcttccgccg gctttccggg gaaagcttgc cgatggaaag accgaaggct tcggcggcat
     2521 cgatcgcctg gatgccgatg gccccggaga aggtgatgat gccgaggcga ttgccccggg
     2581 gcagaggata acggagaaac ggcgtggcgc aatcgcgaag atcttcatat tcctcaaccc
     2641 tcagagcgcc gccctgccga aggagcgcgt catacaggtc agcgttcccg gccagagaac
     2701 cggtgtgaga ggccatcgcc tgcgccgcgc cgggagagcc gcccggcatg aggcacagga
     2761 ccggcttttt cctcgatact ttctttagaa cgtcgagcag catccgcggt cttcgagagt
     2821 gctccatgta cagggagatc acgtccgtgc tcgggtcctc gccgagatag tccaggcagt
     2881 ccgtctcatc gacatcgcac atatttccca gatcaatgac tttgttgatt ccggcttcgt
     2941 attcatgcca cgccatggcc tgcgggctgt acatgccggt ttgggtgatg atggaaagtc
     3001 ttccctgttt ttggtactca tagccgcggt cgtagggaac cgtggtgaac cggctttcgg
     3061 agctgaagac cccgacggtg tttggtccca tgacctgaat gccgtgggat tttgcgattc
     3121 caagcagctt ttcctgccgg attctgcctt ccggccccgc ttcgctgaag ccgtcggaaa
     3181 cgatgacggc ggcctttacg cccttcgcgc cgcactgttc gagaatgccg ggcgcgtgcc
     3241 tgaaggaggt gacgatgacg gccagttcgg gaatgtcctt cacatgggcc agatccggaa
     3301 agaccgggat tccgtaaacg gttcgggcgg cgggatgaac gggatagatc tttccccggt
     3361 agttccattt cagcaggtcg ctgatgatga cggctgcgcc gaaaaaccag ccttcctgca
     3421 tgctgccgaa cagggcgacg cttccgggac ggaaaaacgg atccagacgg tgagcctttc
     3481 gcttcatgcc ggtgctcctt tagggaagtg acgtgtggcg ttacccttat cgcaaccggc
     3541 aaaacatttc aaatggtaat tggcccggcg gatttcgcgc tgatccgggc cggcaatttc
     3601 ccgccgaagg aaccgctgcg cgcaggcggg ggggaataaa aaaagcgcgc cgttttgcgg
     3661 cgcgcttttg cggatgtttt cgcctgaccc ttagagggct ctgacatttt ccgcttccat
     3721 gccctttttg ccctgaacca cgtcaaagct gacctgctgg ccctcggtga gggttttgaa
     3781 gccgctggac tggatagcgc tgaagtgcac gaaaatatca ctcccgccct cttgctcgat
     3841 gaagccgaag cccttgcgtt cgttgaacca ctttactttg ccttccgaca ttgtgctcgt
     3901 cctcctttct caatatttcc taagtcgaat gatcacaacg gcagaaacaa aaaggccgcc
     3961 aggttttaaa gtacttggcg gccgaccttg tgctttattc atgacacgat tcaacttatt
     4021 ccttcgcttt ttttacgttg acccgcgaag ttgggcgcac cctagaggag ttctccgcgg
     4081 ttgtcaagct ttatttttat accagtcgca ttctgcggct aactttgttg tttgtgtcgt
     4141 cggcttcctc gacgtatata aaaatacgcc tgcggagcct tctccttgcc gcctcgttat
     4201 cctttgaatg caacttggta taaatatcct cgcaaaagtt agtgttaaga atggcctatt
     4261 tgttattccg gaccggaagc gcccgcatgc cgtaggtgtg cgactggaga taccagtcgg
     4321 gcctcccgta aatgaaattg tacaaagagg cgtcggcgct gcgcgtttcg gacgaccagg
     4381 cggcaaagca ggtcacctgg atcagctccg tggtcatgtg aatgttgaaa ttgccggcgc
     4441 acggcgcctc gcgggtctgc cgcgcgtcgt acaaaccggc gagttcctca gaggtcggca
     4501 tccgccagtc cttgtaatga ccggcggcgt aattctcgca gtaggatttc gcgccggtcc
     4561 agttgacgtc gctgccgttg tctctggccg cccacatcag gtttgttttg gtgtcgagga
     4621 cggttccgtt cgtgtgatgg acgataaaac ggtcctggct cgcacaggct gacgcgatga
     4681 aggcgatgat cgccatcacc agtattttgc gaagatgctt tttcatggga atctccttat
     4741 cgttcgggga aagtaaaacg aatcaaatcc gcctttcctg catgcgccaa aagtatagga
     4801 aaagcgggtg aacctgtcaa gtgaatcttg gctctcaaat cctggctatg gccgagttcg
     4861 gcgtaaatga aaatattccg cgttaatttt tgcgccaaaa cgtgttatgg ctccgcaagc
     4921 ggccaaacag gcccattggc cgaaggtcgc ttgaaggcgg cagcggatgt tgttgacgaa
     4981 acgcttcccg gcattatatt gaacgggtgg cggccgcgga tcaaaaacac gataaggagc
     5041 aacgataaag atgatgtctg aaaatcgccc tgcaaaattt gccggggcat cacgctatgt
     5101 gctggatgat gagcttgcta aaattgttaa tatttccatg gccttggaga tgccgcttct
     5161 gctcaagggc gaaccgggga ccggcaaaac catgctggcg catgccattg ccgaaaacct
     5221 gcgcatgccc cttattgttc tgaatgtcaa atccagcatg aagctggttg acgccctgta
     5281 tcagtacgac acgctgaccc gactcaacga cagccgtttt tccgattccg ggcgggatgt
     5341 gagcaatatc gaggaatata tcaggatggg caaaatcggc caggcgtttg tctccgacag
     5401 gaaagtcgtg ctgctcatcg acgagatcga caaggccgac acggattttc aggatgacat
     5461 gctcgacatc cttgaccaga tgcagttcga tatcatggaa atcgaccgga cggtgaaagc
     5521 gaagagccgg ccggtgatcg tgatcacgtc caacgccaag aaggatctgt ccgatccgtt
     5581 tctggggcgc tgcaatttcc atcacatcgc tttccccgac cgtgaaatga tgcgcaggat
     5641 catcggcgtg cattttccgg atttgaacag ggatctgacc tcggcctgcc tggatacgtt
     5701 ttaccgcctg cgcgaggtgc gcggcgtgga aaaaaagccg gccacgcgcg aactgatcaa
     5761 ctggatgcgc gcgctccagg ccgatccgga tttcaatgta aaaagcctgc ggtcggccgg
     5821 catccctttt ttgggcgtgc tgttcaaaaa aagcaccgac ctttacctgg ctgcgcacca
     5881 gtaaaaaaag tccggcaggc gccgccgccg ggttgccctt gcgggcgggc ttccatgccc
     5941 gcgccgggtt ttcgggagac gagacatgtt tgtcaatttt ttctacacgc ttcgtgacaa
     6001 gggtgttccc gtgacgccca cgtctttcct gcgtctgcag aaagcgctgg gaatggggct
     6061 tgtcgcctcg ctggacgatt tttacagcgt cgcgcgcagc cttctggtca aaagcgaaaa
     6121 atactttgac atctatgatc aggcgttttc cgccgctttt cggggcatgg ccgatgtcga
     6181 tcccaccgac gcggagctga ccgaggccgt ccaggcgatg ctttccgagt ggctcaggga
     6241 tccgggcggc atggcccggg cgctggggct ttccgaagaa caggtgaaac agatgacgcc
     6301 cgacgaacta gtgcggcatt ttctcaggcg cctcaaggag cagaccgaag cccatcacgg
     6361 cggcagctac tggatcggtt cgcgcggcac atcgcccacg gggcactccg gcgatcatcc
     6421 gggcggcatg cgggtgggcg gccagtcgcg caacaagtcc gccatcaagg tggcgatgga
     6481 gcgccgctac cgggattatt cgcagtccgg ccccatcacg gcttcgcaga tgggcgaggc
     6541 gctcaaaagg ctgaagcacc ttcagcctgc tggcccccgg gacgtcgtca acatcgacaa
     6601 aacgattgat tcgacgatgc gcaacgcggg ggaaattgaa atcatttttg accgccgaat
     6661 ggccgaccgg ctgaaagtca agctgatgat cgacaacggc ggctggtcga tggatcccta
     6721 cgtggaaatc gttcaggcgc tgttccatta cgcgcagttt cagttcaagg aactggagat
     6781 ttattacttt cacaacacga tttattccaa aatctggcag gatccggccc gcagcgccaa
     6841 accggtgcgg gtggatgaat tgattcgctc cgacccggaa acgcggctga ttatcgtggg
     6901 ggacgcgtcc atggcgccct atgaactcaa tgacgcgcac ggagcgatct acctcggcca
     6961 gaatgaagcc cggccttcca tggattatct gaagtttctg tcccggacgt ttcgccactc
     7021 ggtctggctc aatccgcagc cgcaggattc gtggttttac acatggacgg tgaaacagat
     7081 tgccgggatt tttccgatgt tcgagctgtc gctggacggg ctggataaag ccgtccaccg
     7141 cctgagatcc cgaaattgac gaggaggagt catgagaaaa caaattgctt tgataatgtt
     7201 gctggtgttt ttagccctgc cggccctgct tttcgcccaa acgcagccgc cgcgggtggg
     7261 cgccgcgctg cccgacctga aaatccagaa acccgcggat tcggccggcc tgaaatatct
     7321 gggcctctcc ggctccggaa cctttgccgt ggatcaggtt cgcgcccagg cgttgatcat
     7381 ccagatcttc agcctctact gcccttactg ccagaaggac gcgcccaaca tgaaccgctt
     7441 tttcggcctg atcgagggga atccgcgtct caggggaaaa atcaagatcc tcggcatcgg
     7501 cgcgggaaat acgcagtttg aagtgaacac gtttaaaaag aagtacaagg ttgaatttgc
     7561 gctggttccg gacggggatt ttaaaattca caaaatcatc ggcgaagtcc gcactccgta
     7621 tttcatcatc gtgaaactgg acggccccaa aaaacgggaa gtggtttatt ccaggctggg
     7681 cgttcacgaa gacgtggaag cgtttctggc cgaaacggtc cgcctgaccg gaattcagta
     7741 aacgggaggc ctccatgaaa agaaagattg catgcgcgtt gctctttgcc gccttgttcg
     7801 ccgctccggc gttcgccctt tccgacgcct acaagggaaa tgtgttcaat accggcaggc
     7861 tcaagccggt ggacagcgtt ttaaaagtca aagtcggcca ggccgccccg gacttcacgc
     7921 tgcgggccgt aagcggcaaa aaggtgtcgc tcacagacta ccggggaaaa aagaatgtcg
     7981 tgctttcctt tgttcccgcc gcctggacgc cggtttgctc cgaccagtgg cccgggtaca
     8041 acgtagtgca ggagctgttt gaagaaaacg acgccgtgct cctgggcatc agcgttgaca
     8101 acattccgac gcttcattcc tggaccagcc agatggggca gctctggttt gacgtgctgt
     8161 ccgatttctg gccgcacggc gcggcggctt cccgttacgg cattctgcgt tccgacggca
     8221 cggcggagcg ggcgatcatc atcatcgatc gaaaaggcat aatccgctac attgatgttc
     8281 atgacatcaa cgagcgtccg accctggaaa gcattatcgg gcagttggaa aaactgcgga
     8341 aataagtttt ttaattttcc ttgacagggg ttttcaattg cggtacgcac tatgactgta
     8401 agtcataagg ccgttctgtt ggtatttgcc aagcattaac agagacttgc aaaaaggacg
     8461 atgaaaggcc ttcattctgc attcgaagga gagccgctct tgagctcgga agaaagaaga
     8521 aaaaaagaaa aggaaaaccg gaaaaattcc attttgaaag ccgcccgaaa gctgtttttt
     8581 gaacggggtt tcaaatccgt cacggttgac ctgatcgccg ccaaggcgga ggtgagcaag
     8641 ggctccatct acctttactt cgacagcaag gaagaaattt acacccagat cctgatttca
     8701 gccaacatcg accgccacaa ggaatgggat gttttttcca aacgggaggg aaccgcttcg
     8761 gaggccctga tcgctttcgc ccatgaatac gtaagctact ttctggataa taacgaattg
     8821 ttccggatcc tgatgacctt tatgctgcag gcggaaaaca tgaacctgac ggatgagcaa
     8881 aacacacagc tcattcatac gaccaatgaa aatgtccacg tgatcgcgga aattcttcag
     8941 aggggcgtgg agtcgggaga atttattgcc ggcatggacg tccggcgaat gcaattcgcc
     9001 atctggggtc ttctcaacgg agtaatttct ctttatattt acaccggcgc gcccgaaaga
     9061 aggggcgaac gcatcttcac gattgtccac gacagcctca acgctttgct caaaggcata
     9121 aaatcttaag atccgactgt cttcccggtt tttccttcct cctggaggag tttctgaaaa
     9181 tcctggtcgg ccgtgtgcag aagccgctcc acgtcttttt ccagtctttc aaagcgctca
     9241 atgatggatc gggcctgccc ggtgagctgc atggacttgt cgtgcacgcc gatttccacc
     9301 agcttcattc ccatccgctc ttccgaagct ttcaatcgcc cccatgccgc ccgataggac
     9361 atttccagct ttttggcggc ggcgttgagg ctacgctgct catcgatggc cttgagaatg
     9421 tcatcccgtc ccttgccgaa gacgactttt ccgttttttt caatccaaac cttgtatttg
     9481 atttccatgg gaagaactcc tcctgcctgc aggactcgcg aatgtctatt gtatagggaa
     9541 gttgggcggg aaatgcaatc aaatcattat cccgccgtcc ggtttctgat gtatgtttga
     9601 caaaacatat tgacttttgc atgactttcg gtcaacatat gccatgtatg acatagatca
     9661 aaaaagacac atgcatcggg aggattttta tgcaaattac aagaagaggg tttttaacca
     9721 tgaccggcgc catcggaggc ggggttgccc tgtcgagcct ggggctgaac ctcaatccga
     9781 cccgggccta tgccgatgag cttggcaaaa tggatcggat caaaatagcc aaacaggtta
     9841 cgacgatctg ctgttactgc tccgtcggct gcggtctggt ctgcagcgtg gacaaagcga
     9901 cagggaaaat cttcaacatc gaaggcgacg cggatcatcc catcaacgag gggtcgcttt
     9961 gcgccaaggg cgcgggcttc ttcgatctga cggaagccaa caaacaccgc ctgaccaaag
    10021 tgctttaccg caaaccttac gggacggaat gggaagaaaa agactgggac tttgcgcttt
    10081 cccggattgc ccgtctggtc aaggacacgc gtgataagaa ttttacaaag agaaatgaca
    10141 agggcgaact ggtcaaccgc tgcgaggcga tcggccacta cggcagttcc aacgtggaca
    10201 acgaagagtg ctggatcatg agcgtcaagt cccgcgcgct gggtatggtt tatatagacc
    10261 atcaggccag ggtctgacac agtccctctg tagcggctct gggagagtcg ttcggacgcg
    10321 gttccatgac gaaccattac tgtgatttga agaatgccga tgttatcctg attatgggca
    10381 gcaatgccgc cgagcatcac cccatcgcct ttaagtggat tttgagagcg aaagaaaaag
    10441 gcgcaacaat cattcacgtt gatccccgct acacaagaac gtccgcccgc tgcgatttcc
    10501 atgtgccgct tcgttccggc acggacatcg ccttcctggg cggcatgatc cattacattc
    10561 tggaaaacaa gaagttcttc aaggattatg tcctcaatta caccaacgcg tcgttcatcg
    10621 tggacaacgg ctttaatttc aaggacggtt tgttttccgg ttatgatccc aagaaaagaa
    10681 agtacgataa agccacatgg gtcttcgata aagacggaaa aggcattccc cagagggata
    10741 tgagccttac gcatccgcgc tcagtgtttc aattgctcaa aaagcattat tcgcgttaca
    10801 cgctggataa agtttccagc atcacgggcg tgtccaaaga aaacctgctt aaggtttatg
    10861 aaatctacgc ttccaccggt gtgccggaca aggcgggaac ggaatgctac gctctgggct
    10921 ggactcacca taccaccggc agccagaaca tccgcacgat gtcgattatt cagctgctgc
    10981 tgggcaatat gggcatcgcg ggcggcggca tcaatgcgct tcgcggcgaa cccaacgttc
    11041 agggttcgac cgaccattgc attttatacg gcaatctgcc cggctatctg aagatgcttt
    11101 ccgcgtcgct ggatacattg gacaaatatc tgcacaaata cacgccggaa tcgaaagatc
    11161 cccagtcggc caactattat tcgaactatc cgaagttttt tgtcagtttc ctgaaatctc
    11221 tttgggaaga caaggcgaca aaggaaaatg cattcggtta ctcctggctg cccaaaatgg
    11281 acgacgggaa acactattcc accatgcaca tgttcgacgc gatgtatgcc ggaaaggtga
    11341 agggatattt ctgcgttggc gcggatacgg cggtgagcac gccgaattcc aataaagtcc
    11401 gcaaggcgat ggaaaatctg gactggctgg tcggtgaaaa tatcttcgat aacgagactc
    11461 actctttctg gaaaggtccc ggtgtcgatc ccaaaaagat caagacggaa tgcttccttc
    11521 tgcccgccgc cgcaacgatg gagaaggagg gatcgcagag caattccggc cgctgggtgc
    11581 agtggaaata caaggctgcc gaagcccccg gcgacgcgct tcccgtgggt gaaatcgaaa
    11641 tcaagatcat ggcagccgtc aagaagctgt atgaaaaaga aggcggtccc aatgccgaag
    11701 ccattgtcaa tctcaagtgg gattatttgg atagtaaagg gcactttgat gtgattaaag
    11761 tggccaaacg gatcaacggt gttttcctgg aagatacggt gattgaagac aaggcgaaag
    11821 gaacgaagac cgagttcaag aagggtcaac tggttccggg ctttggcaat ctccaggctg
    11881 acggcaaaac ggcctgcggc aactggtgca tctcaggcag ttacacggcg gacggcatca
    11941 acaagatggc gtctcgggga aaggaagatc cgacgggtct cgggcttttc cccaactggt
    12001 cgtttgcctg gccggtcaac cgccgcattc tctataaccg cgcctcctgc gatgtgaacg
    12061 gcaggcccta caatcccaaa cgcaagcttc tggaatggac gggagacaag tgggcaggcg
    12121 acgtgccgga cggaccctgg ccgccgcagg cggacagagc gaaaggaaaa tatcccttca
    12181 tcatgcagag agacggtctg ggcgctctgt tcggacccgg catggccgaa ggcccctttc
    12241 ccgagcatta cgaaccgctg gaaggaccgc tggagaaaaa tttattgtct tcgcagcgta
    12301 tgaatccggc ggcggaaatc tttaaaagtg acatggatca agtggccaac gccagcgaga
    12361 agtttcccta tgtctgcaca acatactcct gcacggagca ctggtgcacc ggcgcattaa
    12421 cccgctggca ggcgtggctc ctggaaatgc agccggaact ttacgtggag atcggcgaga
    12481 aactcgccag ggaaaaaggc attaaaaacg gcgagcggat caaggtttcc tccatccgcg
    12541 gcgaggcccc ctgcatcgcc atggtgacca agcgcttcaa accgttcacc gttgatggaa
    12601 aagaagttca tcaggtcggc atgcccttca attacggctg gctcttcccc aaggacagcg
    12661 gaagcgacag cacgaatatg ttaaccccaa ccgttggcga cgcgaacacc ttctgccccg
    12721 agtacaaggc gttcatggtc aacgtggaaa agttgtagga gggaagcgac catgaataaa
    12781 caacctgaac tcgtcaaatt gattgacacc accctgtgca ccgcctgccg cggctgccag
    12841 gtggcctgca agcagtggaa tgaactgccc ggcctgaaaa ccaaacagct gggcacctac
    12901 cagaatccgc cggacctgca gtggaacacc tggacgctga tccgcttcca ggaatatgaa
    12961 gacaaaaaag gaaacttcca gtggatcttc cgcaaggacg gctgcatgca ctgcacggac
    13021 gccgcctgcg tgaaggtttg tcccagcggt tcgctctatt atacgccgct gaaaactgtc
    13081 ggaattcatc gcgacaagtg catcggctgc aaggaatgcg tgtcggcctg tccgtttcac
    13141 attcccaagt atgacgcaaa aaccgacaag gtttacaaat gcgacctgtg ctattcccgc
    13201 atcaaggaaa gccacgcgcc ggcctgcgtg aagtcctgcc cgaccggcgc cctgaccatc
    13261 ggcgacaaag acgcgatgat caaaaaagcc tatgccaggg tcaaggagct cggcagcgac
    13321 gccaacgttt acggcgacaa atttgtggac gggacgcatg tcatttatgt tctgcagttc
    13381 aagccggaag tttacgaaga actgccggtc aatcccaaag tgcctctgtc cgtgattgtc
    13441 tggaaagacc ttttgaagcc gctcagcctg ctggctgcgg gcggcgtact gggcggagcc
    13501 ttc
//
LOCUS       DAOPAF010000040        13409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4607_length_13409_cov_82.971095, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000040 DAOPAF010000000
VERSION     DAOPAF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_13409_cov_82.971095"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLQ77_05345"
     CDS             complement(<1..1207)
                     /locus_tag="PLQ77_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10135.1"
                     /translation="MKTTSPKTKTARKRAEEALRHNEEKYREMIETIQDGYFEIDLTG
                     KYTFVNDVICRQLQYSREELIGSDNRKYQDEENAKKTFQAFLDAYTTGKPVPALEMDV
                     IRKDGSTQIAEVSISLIRDAGGQPLGFRGISRDITERKQATQALLESEALQRHLMESL
                     PIGLVVVDPRTRMIEHANQTASVMFGAPVDQIVGHRCHAFLCPAQEGACPVCDLGKDV
                     DNSERWMVCADGHHRPVLKTVRKIKIHGREKLLECFVDISERKQVETALKASEERFRG
                     MVESLPDPYSEINLEGIITFVNEAFVHETGYSQDELIGKKFSMLLDEKNARLAKRGYA
                     EIYKTGRPVKNLELEWISKNGHAVLAEVSVSPISQADGTVIGFQSIYRDITERRQMEI
                     ALSRAKEAAE"
     gene            complement(1327..2307)
                     /locus_tag="PLQ77_05350"
     CDS             complement(1327..2307)
                     /locus_tag="PLQ77_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HPL10136.1"
                     /translation="MKYIDHGKGGPASCMTLQDGPVPQIKAGEVLIEVDFAGVNRPDI
                     YQRMGAYPPPPDASPVLGLEVSGRIAAVADDVATWTIGDSVCALVPGGGYAEYCAAPA
                     LHCLPVPRGLSLAQASALPENYFTVWSNVFDRGALTPGENFLVHGGAGGIGYTAIQLA
                     KAFGAVVFTTAAGADQMEAVKKLGADVALDYTTQDWQREIENRIGKRSIDCILDFVGG
                     PYIQKNLRLLARNGRLVQIAFMQGAKVELDCLPILTQNLTFTGSMLRPRSSEEKASIA
                     RALHKNVWPLFAAGKLRVLISAVFELAEAAQAHTLMESAAHVGKIILKVR"
     gene            2645..3715
                     /locus_tag="PLQ77_05355"
     CDS             2645..3715
                     /locus_tag="PLQ77_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL10137.1"
                     /translation="MDMKRDYYEDIELFKSGTVLFWSILLLLFLFTLPLYMPSYNIYL
                     FNIILVNVILAVGLNILVGYTGQISLGHAGFFAVGAYGTSIIMMHLHVPFLPAIIIAA
                     LIAAFLGFIIGLPALRLEGPYLAIATLGFGLTIMHIIGRWEVFGGRMGITAPPLDLGI
                     PGSSWSLVLKGDVQIYYLILVIAILLVIGARNLMKTRVGRAFMSIRDDDIAAEVMGVN
                     LTIFKTMSFAVSAFYAGIAGGLYGFVVSFFDPFSFNMILSIIFLVMVVVGGLGSVLGA
                     IMGAVLITYLQYDLLKNVENLPYIGGALVAISKQWFTVIGLANFSGIALGLIMIAIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            3738..4610
                     /locus_tag="PLQ77_05360"
     CDS             3738..4610
                     /locus_tag="PLQ77_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL10138.1"
                     /translation="MFEMLLIEGLAIGACYGLFGVAVAIIYKTSDVVNFAQGEMGMMA
                     TFVAYHIMTAYGWPFYLAFPATLLFAVLLGMAIEICFLRPAKEPTVLGLLILTLGAEM
                     LLMGLAGWKWGAEQKDFPFLLSVHKNVEIFPGFALNEWTIGVFVLTAVVMVLLFLFFR
                     YSKLGIAMRATQQNENAAKIMGIKVERVFSITWGMSSLIGALAAMFFAARSVLDPNFM
                     MEPFMRAFAAAVLGGLTSIPGVVIGGLILGITENFFGYAWPEWKPIVAFVVIILIITV
                     RPSGLFAKHYVKKV"
     gene            4666..5118
                     /locus_tag="PLQ77_05365"
     CDS             4666..5118
                     /locus_tag="PLQ77_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10139.1"
                     /translation="MSGKKTAILLCACAAVLIAGLLIWDAPRNRVQLFWEKGAIEEAA
                     HRYFQAEKAGDLKQTYALLAPSSAYRQAHSYDSYLKDIAGYPPVKIDTYQIVDVYRLR
                     DNDRRDQYPGVDKLVQVEVDVTFVNSGKNNVFNYSFTFLKEQGSWYKG"
     gene            5183..6370
                     /locus_tag="PLQ77_05370"
     CDS             5183..6370
                     /locus_tag="PLQ77_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL10140.1"
                     /translation="MKRVLVAVMILCAVLMIPFAADAGKPGFDDKEIRIAQWGPQTGP
                     AAPWGSVARGSDILFKIINDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIFA
                     FVGGVSAAAGMAVKGYLAENKVIWVGPATAINEYVFPVQPYLFGIYPLYDDEASILTK
                     YAAQKLKAKKIAFFYQNDAYGKSGLDGCKDRMKALKMQLVQEVSTEPGEKDLSSQILR
                     LKNSGADVVIMWVNPTSAVIALKTAATVGFKPQWISSNTLSDYVLMNKITGGLWEGVI
                     TGAFAEAPDSNTPLLKKYREAAKKYAPEERWGTFYMAGILFAEPLVEAIKRAGRNLST
                     DAVLKQLNSMKKFQGIGPPVTWTPKVHQGSDAIMIQQCGPNSSYITLQNWTANELATW
                     KKK"
     gene            6454..7227
                     /locus_tag="PLQ77_05375"
     CDS             6454..7227
                     /locus_tag="PLQ77_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10141.1"
                     /translation="MAHFKVEHLSISFGGIKAVNDVSFEVEHNKIFSIIGPNGSGKTT
                     IFNMISGIYKPGAGEVLLEGENLVGLRPDTIARRGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGILSGAFMWGRHSPSAKEEIRHRELIEHIVDFLDLQSLRDQFVSNLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYQVTILLIEHDMNMV
                     MGISDRILAISQGVKIIEGTPQEVQKHPDVIAAYLGEED"
     gene            7227..8000
                     /locus_tag="PLQ77_05380"
     CDS             7227..8000
                     /locus_tag="PLQ77_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10142.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIQEGQIVALLGSNGAGKTTTL
                     KTIMRILDDDDQPEKGTIEFLGKRIDRMDTEEIVRLGISYVPEGREVFPELTVMENIQ
                     MGAYTRRDRKGIQEDIEGIFKQFPILSERRNQQAGHLSGGEQQMLAIGRALMSRPKLL
                     MLDEPSLGLSPLLTKEIFNIIQRINKQGVTILLVEQNVNMALKYSHYAFLMENGRIVR
                     ADKPEVLREDDDVKEFYLGIATETSVKGYKRYRRKVRFR"
     gene            8011..9828
                     /locus_tag="PLQ77_05385"
     CDS             8011..9828
                     /locus_tag="PLQ77_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL10143.1"
                     /translation="MKKEEIQTLPQGLKFMVQEQPDRIAMRMKDYGIWHDITWAQYYE
                     NVKKVAMGLHALGVGRGDHVAIVGENKPEWLFSAMGVMSLGGIFVGVYTTNPAAECEY
                     IVGHSESVVFFCEDEEQFDKALVFRSRTPHLKKCVVWDMEGLKHFDDPMLMSFDDLMA
                     LGEKTDRENPGLYEKIAAQGKGEETASIIYTSGTTGPPKGAMISHRNYLWIAQATEKI
                     TRMETKDETISFLPLNHVYEQIFDMMMHMWAGHIVNFTENTDTVMNDLRDVSPTLFHA
                     VPRIWEKYHSAIVLKMADATWLKRTVFNLAFKIGSKYNTVKLAKAPMPWYLYIGYQIA
                     YFCVFWKLKERLGFDRVRLGFSGAAPISHEILKFFQSIGIPIREGYGLTETTGITHIS
                     DAQNFKLGTVGRNLPGAKVQIAEDGEILVQHGGIFQGYFKEPEATAAALEGGWFHTGD
                     VGEIDQEGYLKITDRKKDLIITAGGKNIAPQYIENLLKFSPYITDAVVIGDRRKYVTA
                     LIVIDEENVVKFAQDHKVQYTTFASLTKTEEVIKLISDEVEKVNKQIARVENIRKFRL
                     LDKKLYTEDGEVTPTMKVKRKSIAAQYKDVIESMYREGE"
     gene            9930..10394
                     /locus_tag="PLQ77_05390"
     CDS             9930..10394
                     /locus_tag="PLQ77_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:10059..10061,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HPL10144.1"
                     /translation="MARVEINTPAPAFTLNDFSGKSVSLSDYAGQKNVLVVFNRGFMU
                     PFCRAHMAQLRRDYDSFVKLDTEILVAGPETPETFRDYWQKHDLPFVGLPDPEHRVLK
                     LYGQEVKLFKLGRLPAQMLIDRTGKVRFVHYGHSMADIPPNEEILHLIETQL"
     gene            10497..11312
                     /locus_tag="PLQ77_05395"
     CDS             10497..11312
                     /locus_tag="PLQ77_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL10145.1"
                     /translation="MKNLKGRNVLLTGAGSGIGRLMAHYVADEGANTAIIDIQAEALA
                     KTAKEVRLRKVKVKEYVCDISDCVSVGDVVARIKQDFGQIDVLINNAGTVIGKSFFDL
                     TLEEMQRTMNINYWGHIYLTKAVIGDMVARNDGAIVNVASSSGLIGMPILSDYAASKF
                     AEVGFSESLRRELKKFNYTGVTVTCVCPYIIDTGLFKGFKPMAMSPFIKPEVAARAIV
                     EGLKKGKPYVMLPPLSIYSSLMLKLLPTPLFDWILKMSGAEKAMDCFEGRKSC"
     gene            11349..12884
                     /locus_tag="PLQ77_05400"
     CDS             11349..12884
                     /locus_tag="PLQ77_05400"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPL10146.1"
                     /translation="MNLEFANIGEMVQTQGRRYPDRIAIRFYERDITYRELDEQSNRV
                     ANGLLRLGVRKGDRVCMLMDNSPEFYYTYFGINKIGAIAGPVNCWWQIAEIRYLLKDS
                     GAVVLFCDHTYRGHIDKIIGETPDLQHVIEREAADDRFLPFEKMLTEPVALADAAISP
                     DDVSTIVYTSGTTGSPKGVLLTHRNILTNSWQASLLANIDEKDVILCFLPLFHVNGLV
                     ITGTSPLSKGSQIVLRRNFSASEFWECVAKYRVSIFSGVPTVYQILLNTPGFEGLDVS
                     SLRYGVCGAAPMPVETIRKFEETFNMIIVEGYGLTESTAGATANPINGVRKIGSIGIP
                     FAGTEIRILDDEDKEVPQGDVGEICIRGDHVMKGYFNKPEDTAQTLRGGWLHTGDMAY
                     KDEDGYLFIVDRKKEMIIRGGENIYPKELEGIICRHPDVAEVAVVGVPDAIYGEEVMA
                     CLVLHPDRMLAEEAFQDWCRANMASYKVPKYVNIRASLPRNLLGKLLKKELKAALKSE
                     GRI"
     gene            complement(12955..>13409)
                     /locus_tag="PLQ77_05405"
     CDS             complement(12955..>13409)
                     /locus_tag="PLQ77_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPL10147.1"
                     /translation="PFFQMPAVFAASALAGAVILIWMAAGMLRSLPTLRLSWEGGQQK
                     MNSTVLNGILMSVSNPYWIIWWATIGIGYIFYAWQYGLWGIAFFFAGHISADLAWYSF
                     IAGAVARGRHLLTDRLYRRLIGFCALFLIGFAGYFAFAGLVKLKGLLS"
BASE COUNT         3013 a         3662 c         3850 g         2884 t
ORIGIN      
        1 cctccgccgc ctccttggcc cgggaaagcg cgatttccat ttgccggcgc tcggtgatat
       61 cgcggtaaat ggattgaaaa ccgatcacgg ttccatcggc ctgactgatc ggactaacgg
      121 acacttcagc cagcacggcg tggccatttt tggagatcca ttccaattcg agatttttta
      181 cgggacgtcc tgttttgtaa atttcggcgt atccccgttt ggccagccgg gcattttttt
      241 cgtccaggag catgctgaat ttttttccga tcagttcatc ttggctgtat cccgtttcat
      301 gcacaaaggc ctcgttgaca aacgtgatga ttccttccag attgatttct gaataggggt
      361 cgggaagaga ttccaccatg ccgcggaagc gttcttcgct ggccttgaga gccgtttcca
      421 cctgcttgcg ctcgctgatg tccacaaagc attccagcag cttttcacgg ccgtgaattt
      481 ttattttcct gacggttttc agaaccggac ggtgatggcc gtccgcgcag accatccatc
      541 gttcggaatt gtcgacgtct ttgcccaggt cgcagacggg acaggcgcct tcctgggccg
      601 ggcacagaaa agcatggcac cggtgtccga cgatctggtc caccggggcg ccgaacatca
      661 cggaagcggt ttggttggcg tgctcaatca tgcgcgtccg cgggtccacg accaccagcc
      721 cgatgggcag agattccatc aggtgacgct gcagcgcttc gctctccaga agggcctgcg
      781 tcgcctgttt gcgctccgtg atatcccggg agatgccgcg aaaccccagg ggttgtcccc
      841 cggcgtcccg aatgagggat atcgacactt cggcaatttg cgtcgagccg tctttgcgga
      901 tcacgtccat ttccagcgcc ggcaccggtt tgcccgtcgt gtaagcgtcc aaaaaagcct
      961 ggaaagtttt tttggcgttt tcctcatctt gatacttgcg gttgtccgat ccgatcaatt
     1021 cttcgcggga gtattgcagc tgccggcaga tcacatcatt gacaaaagtg tatttgccgg
     1081 tcagatcaat ctcgaaataa ccgtcctgga tggtttcgat catctcgcgg tatttttctt
     1141 cattgtgccg cagggcctct tccgcgcgct tgcgcgcggt ttttgttttc ggggaggttg
     1201 ttttcattgg tcatcgccat ccgttgcgtg ataaagggtt catgacgtat catttcaacc
     1261 tgaacacaca actacctgaa aaaacgtttc aagtaaaggc cgcaaaatca ttgccggcag
     1321 acgcgttcag cgcactttca ggatgatctt gccgacatga gcggccgatt ccatgagggt
     1381 atgagcctgc gcggcttcgg caagctcaaa gacggcggag atcaaaaccc gcagctttcc
     1441 cgcggcaaag agcggccaga cgtttttgtg aagggcccgg gcgatggagg ccttttcctc
     1501 cgaagagcgg ggacgaagca tcgagccggt gaaggtcaga ttttgggtca aaatcggcag
     1561 gcagtccagt tccactttcg cgccctgcat gaaggcgatc tgcaccagcc ttccgtttcg
     1621 ggccagcagc ctcagattct tttgaatata ggggccgccc acgaaatcca gaatacagtc
     1681 gatgctcctc ttcccgatcc ggttttcaat ttcccgctgc cagtcctgcg tcgtataatc
     1741 cagcgcaaca tccgccccca gttttttcac ggcttccatc tgatcggcgc cggccgcggt
     1801 ggtaaagacc acggcgccga aggccttggc cagttgaatg gcggtatagc cgatgcctcc
     1861 cgcgccgccg tgaaccagaa aattttcacc gggcgtgagc gcgccgcggt cgaagacatt
     1921 cgaccagacg gtgaaatagt tttccggaag cgcagacgct tgtgccaggg aaagcccccg
     1981 tggaaccggc aggcaatgca gagcgggcgc ggcgcagtat tcggcatatc cgccgccggg
     2041 cacaagcgcg caaaccgaat ctccgatcgt ccatgtggcc acatcgtccg cgacggcggc
     2101 gattcggccc gagacttcga ggcccagcac aggcgacgcg tcgggcggcg gtggatacgc
     2161 ccccatgcgt tggtagatat ccggacggtt aacacccgcg aagtcgactt caatcagcac
     2221 ttcgccggct ttgatctgcg gcacgggtcc atcctgcagg gtcatgcagg aggcgggccc
     2281 gccttttccg tgatcgatat atttcatggt tcctccgcgt tttccaaaat gctcgcgctt
     2341 tgaccaccgg tgattaacgg tttttcctcc tgccgggcgg gtgcaagaag atgtctttca
     2401 tgccgtccgt cctgatcaaa atgccgtctg atcctagcac accggaaaga aaaaaacacg
     2461 ggccttgttc cggtcccgcc gggtatcgcc ttaaattttc ttttgtttct agaataattc
     2521 tctttgatca tgaaattttc ttgacagaca aatttcgttt ctatatgatt cgccaccggt
     2581 ttacgtcaaa tgaagcagat gcatctaacc ctctagttgg ctacagtagt ggcgtggtga
     2641 tcctatggac atgaagcgcg attactatga agacatcgag cttttcaaga gcggcacggt
     2701 attattctgg tcgatccttt tactgctgtt cctcttcacg ctgcctctct acatgccttc
     2761 atacaacatt tacctgttca acatcattct ggtgaacgtc atccttgcgg tgggcctgaa
     2821 tattctggtg ggttacaccg gccagatatc cctgggacat gccggttttt tcgcggtggg
     2881 cgcctacggc acatcgatca ttatgatgca tttgcatgtt ccttttttgc ccgccatcat
     2941 aatagctgct ctgatcgcgg cttttctggg ctttatcatc ggtcttccgg cgctgcggct
     3001 cgaagggccc tatctggcga ttgccacact gggcttcggc cttaccatca tgcatatcat
     3061 cggtcgatgg gaagttttcg gcggcagaat gggcatcacc gcgccgcctc ttgacctcgg
     3121 aattcccggt tcctcctgga gcctggtgct caagggagac gtgcagatct actacctcat
     3181 cctggtgatc gcgatcctgc tcgtcatcgg ggcgcgcaat cttatgaaga caagggtggg
     3241 gcgcgccttc atgtccattc gcgacgacga catcgccgcc gaggtcatgg gcgtgaacct
     3301 gacgattttc aaaaccatgt cctttgccgt cagcgctttt tacgccggca tcgcgggagg
     3361 cctttacgga tttgtcgtca gcttcttcga tcccttttcc tttaatatga ttctgtccat
     3421 tatcttcctg gtgatggtgg tggtcggcgg tctgggttcc gtactggggg cgatcatggg
     3481 cgccgtcctg attacgtatc tgcagtacga ccttttgaaa aacgtggaaa acctgcccta
     3541 tatcggcggg gccttggtgg ccatctccaa gcagtggttt accgtgattg gactggccaa
     3601 cttctccggc atcgctttgg ggctcatcat gatcgccatt gtgatttttg agccgctggg
     3661 aatgttcggc atctggatcc ggattaaaaa atattggaag acctggccgt tttaaaaggc
     3721 caaaagggag tcttggcatg tttgagatgc tgctgattga aggtctggcc attggagcct
     3781 gctacggtct tttcggcgtc gccgtcgcca ttatctacaa gacgtcggat gtggtgaact
     3841 tcgcgcaggg tgaaatgggc atgatggcca cgtttgtcgc ctaccatatc atgaccgctt
     3901 acggctggcc tttctacctt gcttttcccg caaccctttt gtttgccgtc ctgctgggca
     3961 tggccatcga aatctgtttt ttgcggcccg ccaaggaacc gaccgttctg ggcctgctga
     4021 ttctgaccct cggcgcggag atgctgctga tggggttggc cggctggaaa tggggcgcgg
     4081 agcagaagga ctttccgttt cttctttccg ttcacaagaa tgtcgaaatt tttccgggct
     4141 tcgccctgaa cgaatggacc atcggtgttt tcgtgctgac ggcggtggtc atggtgctgc
     4201 tgtttttgtt tttccgttat tccaaactgg gcattgccat gcgggcgacg cagcagaatg
     4261 aaaacgccgc caagatcatg ggcatcaagg tggaaagggt gttttccatc acctggggca
     4321 tgagctccct catcggcgcg ctggccgcca tgtttttcgc cgcccgctcc gtgctggacc
     4381 ccaacttcat gatggagccc ttcatgcgcg cctttgccgc ggccgttctc gggggcctca
     4441 ccagtattcc gggcgtcgtg atcggcggcc tgattctggg catcaccgaa aactttttcg
     4501 gctatgcctg gccggagtgg aagccgatcg tggcctttgt ggtcatcatc ctgattatta
     4561 ccgtgcggcc cagcgggctg ttcgccaagc attatgtgaa gaaagtctag cgtgttttgc
     4621 cggccgatcc ccgcggggag cggccggagc attcgggagc ttttcatgag cgggaaaaaa
     4681 acagccatcc tcctttgcgc ctgcgcggcg gtgctgatcg ccgggctgct gatctgggat
     4741 gctccccgca accgggtgca gcttttttgg gaaaaggggg cgattgaaga ggccgcccac
     4801 cggtatttcc aggcggaaaa ggccggggac ctgaagcaga cctatgcgct tctggcgcct
     4861 tcgtccgctt accggcaggc gcactcctac gattcgtact tgaaggatat tgccggctac
     4921 ccgcccgtaa agatcgatac ttatcagatt gtcgatgtct atcgcctgcg ggacaacgat
     4981 cgccgggatc agtatcccgg agtggacaag ctggttcagg tggaagtgga tgtgaccttc
     5041 gtcaattcgg gcaaaaacaa tgtttttaac tacagcttca cttttctcaa ggaacaggga
     5101 agctggtata agggatgaag cgcaaaaggg tatgaagaaa aagcaccgtc aacattccac
     5161 aagaggaaca aaaggagggg ttatgaaaag ggtactggtt gcagtcatga ttttatgcgc
     5221 ggtcctgatg attccgtttg cggccgacgc gggcaaaccg ggatttgacg acaaggaaat
     5281 ccggattgct cagtgggggc ctcagacagg gcccgccgcg ccctgggggt cggttgcccg
     5341 ggggagcgac attctgttta aaatcatcaa cgacgaaggc ggcattcacg gccgcaaaat
     5401 caaatacttc atccgcgacg atcagtacaa tcccgcccag accaaggcgg tggtgaagga
     5461 actggtggag cgcgaaggca tttttgcctt tgtcggaggc gtgagcgcgg cggccggcat
     5521 ggcggtcaag gggtatctgg ccgagaacaa agtgatctgg gtcggtccgg ccacggccat
     5581 caacgaatat gtattccccg tgcagcccta tcttttcggg atttaccccc tgtatgacga
     5641 cgaggccagc attcttacca aatacgcggc ccagaagctc aaagccaaaa aaatcgcttt
     5701 cttctaccag aatgacgcct acggcaaaag cggcctggat ggatgcaagg accggatgaa
     5761 agccctcaag atgcagctgg tgcaggaagt ttccacggag ccgggcgaaa aagacctgtc
     5821 ttcccagatc ctgcgcctga aaaattccgg ggccgacgtc gtgatcatgt gggtcaatcc
     5881 cacctcggcc gtgatcgcgc tcaagacggc cgcgaccgtc ggcttcaaac cgcagtggat
     5941 atcctccaat acgctttccg actacgtgct gatgaacaag atcaccggtg gattgtggga
     6001 aggcgtgatc accggagcgt ttgccgaggc gcccgattcc aacacgccgc ttttgaagaa
     6061 ataccgggaa gccgccaaga aatacgcgcc ggaagaacgc tggggcacct tctacatggc
     6121 gggcattctc tttgccgaac cgctggtcga agcgatcaaa agagccggcc gcaacctcag
     6181 cacggacgcg gtgctcaagc agctcaactc gatgaaaaag tttcagggga tcggcccccc
     6241 ggtgacctgg acgccgaagg tgcatcaggg atcggacgcc attatgattc agcagtgcgg
     6301 ccccaattcc tcctacatca cgctgcagaa ctggaccgcc aacgagttgg caacctggaa
     6361 gaagaaataa ttcaagacgc cgggggaagg cgggtcgaac cgccttctcc cggcgataac
     6421 caccggtggt ggcggtgacg ggttaaaagg cttatggcgc actttaaagt ggaacacctg
     6481 agcatctctt tcggcggcat caaggcagtc aatgatgtca gcttcgaagt ggagcacaat
     6541 aaaatctttt cgatcatcgg accgaacggc agcggcaaga ccacgatctt caacatgatc
     6601 agcggcatct acaagcccgg cgccggagag gtgctgctgg aaggcgagaa tctggtcggc
     6661 ctgcgtccgg acacgatcgc ccgcagaggc gtggcgcgga cgtttcagaa cattcagctt
     6721 ttcggcaacg ccacggtcat ggacaacctg atgctggggc ggcacatcca catgaaaacg
     6781 ggaattctca gcggcgcttt catgtggggg cggcattctc cgagcgcgaa ggaggaaatc
     6841 cggcatcgcg aactcatcga gcacatcgtc gattttctgg acctgcagtc cctgcgcgac
     6901 cagtttgtgt ccaacctgcc ctacggcaag cgcaaactgg tggagctggg gcgggcgctg
     6961 gcccttgagc ccaaggtgct cctgctggac gaaccgtccg ccggcatgaa caccgaagaa
     7021 aaggacgatt tgcgcatctg gatcaaggac attcaggaag actatcaggt gacgattctg
     7081 ctcatcgagc acgacatgaa catggtgatg ggcatctccg acaggattct ggccatcagt
     7141 cagggcgtga agattatcga gggcacgccg caggaagtgc agaagcaccc cgacgtgatt
     7201 gccgcctatc tgggagagga ggactaatgc tctcggtcaa aaatattgaa acctggtacg
     7261 atctgatccg ggcgctgcac ggcatctcct ttgaaattca ggagggtcag attgtggcgc
     7321 ttctgggcag caacggcgcg ggcaaaacca cgacgctcaa gacgatcatg cggattctgg
     7381 acgacgacga ccagccggaa aaaggcacga ttgagtttct gggcaagcgc atcgaccgca
     7441 tggacacgga ggaaatcgtc cgcctgggca tcagctacgt gccggagggc cgcgaggtct
     7501 ttcccgagct gacggtcatg gaaaacattc agatgggcgc ttacacgcgc cgcgaccgca
     7561 agggaattca agaagacatt gaaggcattt tcaagcagtt tcccattttg agcgagcgca
     7621 ggaaccagca ggccgggcat ttgagcggcg gggagcagca gatgctggcc atcggccgcg
     7681 cgctgatgag ccgtcccaag ctgctgatgc tcgacgagcc gtcgctgggc ctgtcgcctc
     7741 tgttgaccaa ggaaattttc aacatcatcc agcgcatcaa caagcagggc gtgaccatcc
     7801 tgctggtcga gcagaacgtg aacatggcgc tgaaatattc gcattacgct tttctgatgg
     7861 aaaacggccg gatcgttcgg gccgacaaac cggaagtgct gcgcgaggat gacgacgtga
     7921 aggagtttta tctgggcatt gccacggaga cctccgtgaa aggctacaaa cgttaccgcc
     7981 ggaaagtgcg tttccgctaa ggaagaaact atgaaaaaag aggagattca aaccctgccg
     8041 cagggcctca agtttatggt gcaggagcag ccggaccgga ttgccatgcg catgaaggat
     8101 tacggaatct ggcacgacat tacctgggcc cagtattatg aaaacgtgaa aaaagtcgcc
     8161 atgggactgc acgccctggg cgtcgggcga ggcgaccatg tggcgattgt cggcgagaac
     8221 aagcccgaat ggcttttctc ggccatgggc gtcatgtcgc tgggaggaat cttcgtcggc
     8281 gtttacacaa ccaacccggc cgcggaatgt gaatatatcg tcggccattc cgagtccgtc
     8341 gtcttcttct gtgaggacga ggaacagttc gacaaggcgc tggttttccg ctcccgaacg
     8401 ccgcatctca aaaaatgcgt ggtttgggat atggagggcc tgaaacattt cgatgatccc
     8461 atgttgatga gttttgacga tctgatggcg ctgggagaaa agacggaccg ggagaatccc
     8521 ggtctgtacg agaaaatcgc ggcgcagggg aaaggcgaag aaaccgcctc catcatctat
     8581 acctccggca ccaccggccc cccgaagggc gcgatgatca gccatcggaa ttatctgtgg
     8641 atcgcgcagg cgacggaaaa aatcacccgg atggaaacga aggacgagac catttccttc
     8701 ctgccgctca accatgtcta tgaacagatc tttgatatga tgatgcacat gtgggcgggc
     8761 catattgtga acttcacgga aaatacggac acggtgatga acgatctgcg cgacgtgtcg
     8821 cccacgcttt ttcacgccgt tccccgcatc tgggaaaaat accactccgc catcgtgctc
     8881 aagatggccg acgcgacctg gctgaaacga acggtcttca acctcgcctt caaaatcggc
     8941 tcaaagtaca acaccgttaa actggcgaaa gcccccatgc cctggtatct gtacatcggc
     9001 tatcagattg cgtatttctg cgttttctgg aagctcaagg agcggctcgg ctttgaccgc
     9061 gtccgcctcg gattttccgg cgcggcgccc atctcgcacg agattctgaa gtttttccag
     9121 agcatcggca ttcccatccg cgaaggctac gggctgaccg agaccacggg catcacgcac
     9181 atctccgacg cgcaaaactt caagctgggg acggtggggc ggaatctgcc gggcgccaag
     9241 gtgcaaatcg ccgaagacgg cgagattctg gttcagcacg ggggcatttt ccagggatac
     9301 ttcaaggaac ccgaagccac ggccgcggcg ctggagggcg gctggttcca caccggcgac
     9361 gtcggggaaa tcgatcagga aggctatctc aagatcaccg accgcaaaaa agacctgatc
     9421 atcacggcgg gcgggaaaaa catcgcgccg cagtacattg aaaacctgct gaaattctct
     9481 ccgtacatca cggacgcggt ggtcatcggc gaccggcgca agtatgtcac ggcgctcatc
     9541 gtcatcgacg aggaaaacgt cgtgaaattc gcgcaggacc acaaggtgca gtacacgacg
     9601 tttgccagtc tgaccaagac cgaagaggtc atcaagctga tttcggacga ggtggagaag
     9661 gtcaataagc agatcgcccg cgtggaaaac atccgcaagt tccgcctgct ggacaagaag
     9721 ctctacacgg aagacggaga agtcaccccg accatgaagg tcaagcgcaa atccatcgcc
     9781 gcgcagtaca aggacgtcat cgagtccatg taccgggaag gggaatagtt ttttcacgtt
     9841 cccaagaagc cggggctgaa tttgaactga aggcggaggt gcgagaaccg gcaatacttg
     9901 aacgctgacg ccggaaggag gcgttcctta tggcccgagt cgaaatcaat acgcccgcac
     9961 ccgctttcac actgaacgat ttcagcggca aaagcgtgtc cctgtccgat tatgccggac
    10021 agaaaaacgt tctggtcgtt ttcaaccgcg gcttcatgtg accgttctgc cgcgcgcaca
    10081 tggcgcagtt gcgccgggat tacgattcgt tcgtgaagct cgatacggaa atccttgttg
    10141 caggccctga aacacccgag acattccgcg actactggca aaaacatgac ctgccgtttg
    10201 tcggccttcc ggatccggag caccgggttc tgaagcttta cggccaggaa gtgaagctct
    10261 tcaagcttgg ccgtctgccc gcgcagatgc tgatcgaccg cacgggaaag gtccgcttcg
    10321 tgcactacgg ccactccatg gcggacattc cccccaatga ggagatactc catttgattg
    10381 aaacgcagct atgaagcaac accctcatag accgaactgc cgggaaaaga gtcgatgccc
    10441 caaaccgatg aaaaaaaata aatgatgagg ccggaataat tcagcaggaa gtgcgaatga
    10501 aaaatctgaa aggcaggaat gttctcctta cgggcgccgg aagcggcata ggtcggctga
    10561 tggcccacta tgtggccgat gagggcgcca atacggccat aatcgatatt caagcagagg
    10621 cccttgcgaa aacagccaag gaggttcggc tgcggaaagt caaggtgaaa gagtatgttt
    10681 gcgatatatc cgattgtgta tccgtcggag atgtggttgc ccggatcaag caggatttcg
    10741 gacaaattga cgtgctcatc aacaatgccg gcactgtaat cggaaaatca tttttcgatc
    10801 tgacgctgga agagatgcag cgcaccatga atatcaatta ctgggggcac atctatttaa
    10861 ccaaggccgt catcggagac atggtggcgc gcaatgacgg ggcgattgtc aatgttgcat
    10921 cctccagcgg tcttatcggc atgccgatcc tgtcggatta cgccgccagc aaattcgccg
    10981 aagtcggatt ctccgaatcg ctgcggcggg aactcaaaaa attcaattac accggcgtca
    11041 cggtgacctg cgtttgcccg tacatcatcg acaccggtct gttcaagggt ttcaaaccca
    11101 tggcgatgag cccttttatc aagcctgagg tcgcggcgcg tgcgattgtg gaagggctca
    11161 agaagggcaa gccttatgtc atgctcccgc ctttgagcat ttacagttcg ctgatgctga
    11221 aacttctgcc tacacccctg ttcgactgga tcctgaagat gtccggcgcc gagaaggcga
    11281 tggactgctt tgaaggcaga aaatcatgtt aaattagaaa tgttgatcat catggaaagg
    11341 agatcagcat gaatctcgaa tttgcgaaca tcggggaaat ggtgcaaacg cagggtcgcc
    11401 gctatccgga cagaatcgcc atccgctttt acgaacggga catcacgtat cgtgaactgg
    11461 atgaacagag caaccgcgtg gccaacggtc ttcttcggct gggcgtccga aaaggcgacc
    11521 gggtctgcat gctcatggat aacagcccgg aattttacta tacttatttt gggatcaaca
    11581 agatcggggc catcgccggt ccggtcaact gctggtggca gatcgcagaa atccgttacc
    11641 tgctgaaaga ttccggagcg gtggtcctgt tttgcgacca cacctaccgg gggcacatcg
    11701 ataagatcat cggtgaaacg ccggatctgc agcacgtcat tgaacgggaa gcggccgacg
    11761 atcgtttttt gccgttcgaa aaaatgctga cggagccggt ggctcttgcg gatgcggcga
    11821 tcagccccga cgacgtctcc acgatcgtct atacctcggg aaccaccgga agcccgaagg
    11881 gagttttgct cacccacagg aatatcctga ccaattcctg gcaggccagt ctgctggcca
    11941 acatcgatga aaaagacgtc attttgtgct tcctgccgct cttccacgtc aacggcctcg
    12001 tgattaccgg gacatctccc ttgagcaaag gttcacagat cgtcctgcgc cgcaacttct
    12061 ccgcctcgga gttctgggaa tgcgtcgcga agtatcgcgt ctcgattttc agcggcgtgc
    12121 cgacggttta ccagattctc ctgaatacgc cggggtttga aggattggac gtgtcgtcgc
    12181 tgcgctacgg cgtttgcggc gcggctccca tgcccgtgga gaccatccgg aaatttgagg
    12241 aaacctttaa catgattatc gtcgagggat acgggctgac cgaaagtacc gccggggcga
    12301 cagccaaccc catcaacggc gtccggaaaa tcggctccat cgggattccc tttgcgggaa
    12361 ccgaaatccg gattctggac gatgaggaca aggaggtccc ccagggagac gtgggagaaa
    12421 tttgcattcg gggcgaccat gtcatgaaag gctacttcaa caaaccggag gacaccgccc
    12481 agaccctgcg cggcgggtgg ctccacacgg gggacatggc ctataaagac gaggacggct
    12541 acctgtttat cgtggaccgg aaaaaggaaa tgatcattcg gggcggtgaa aatatctatc
    12601 ccaaggagct ggagggcatc atctgccggc accccgacgt cgcggaggtt gcggtggtcg
    12661 gcgtccccga tgcgatctac ggggaagagg ttatggcctg cctggttctc catccggacc
    12721 ggatgcttgc ggaggaagcc tttcaagact ggtgccgggc gaacatggcg tcctataagg
    12781 tgccgaaata cgttaatatc cgcgcatccc tgcccaggaa tcttctgggg aagcttctca
    12841 agaaagaact gaaagccgcc ctgaagtcgg aggggaggat ctgaaacagg acaaagcgag
    12901 ataaaacagc tgagcaaacg ggcgacggga attctggatt tgatccggtc gcggttatga
    12961 cagaaggcct ttgagtttga caaggcccgc gaaggcgaaa tagccggcaa aaccgatcag
    13021 gaaaagggcg caaaatccga tcagccggcg gtagagccgg tccgtcagca ggtgcctgcc
    13081 ccgggcgacc gcgccggcga tgaaagaata ccaggccagg tccgccgaaa tgtggccggc
    13141 gaagaaaaag gcaattcccc aaaggccgta ctgccaggcg tagaaaatat acccgatgcc
    13201 gatcgtcgcc caccagatga tccagtaggg attggaaacg ctcatcagga ttccgttcag
    13261 taccgtagag ttcatttttt gctgcccgcc ttcccacgac agccgcaggg tgggcaaaga
    13321 ccgcagcatt cccgccgcca tccagatcag aatgaccgcg ccggccagcg cgcttgccgc
    13381 aaaaaccgcc ggcatctgaa aaaacgggg
//
LOCUS       DAOPAF010000041        13217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_4681_length_13217_cov_66.322443, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000041 DAOPAF010000000
VERSION     DAOPAF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_13217_cov_66.322443"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1279)
                     /locus_tag="PLQ77_05410"
     CDS             complement(152..1279)
                     /locus_tag="PLQ77_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPL10148.1"
                     /translation="MALSPLKYRHSILLIDDEEAILEIMAAMLADEGYDLLTAGSAEE
                     GLALLKKTPDVSLIISDQHMPGMTGVQFFAQARHISPDALRVLLTGYTDTDAIIDAIN
                     SGGIHRYLTKPWSREDFLHTIRQMLGKADLMRENRRLDELVKKQNAELLDLNKGLEEK
                     VRQKTRHLNQSLEELRSSLEKIRMILGGTVLAMSRIVESRDPYTAGHEQQVARIACVI
                     SEKLLLPVDRVEAIRIAGQLHDIGKIAVPSEILTKPGRLSPLEMEMVKTHCRNAYDIL
                     KAIEFPYPVAKIILQHHERMDGSGYPQGLKGDDILLEARIIGVADVIEAISSHRPYRP
                     AMDIDTAMKEITDHRGILYDANVVDACLDIYRTQGRQGFLS"
     gene            complement(1423..4260)
                     /gene="uvrA"
                     /locus_tag="PLQ77_05415"
     CDS             complement(1423..4260)
                     /gene="uvrA"
                     /locus_tag="PLQ77_05415"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPL10149.1"
                     /translation="MDCIRIKGACQHNLKKVSLDIPRDRLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFIGQMDKPEVESIEGLSPAIAIEQRAAGHNPRSTVGTVT
                     EIYDYFRLLFAGIGVCHCSGCGREIHSQTIDSILHSVLDLPEGTRFSILSPLVRGKKG
                     EFAKELKKLQKEGFARVRIDGEIRDLAEDIVLAKTKRHDIDLIVDRLVLKDGIRKRLR
                     DSLEIAAHKSDGLVRIAAAEGRETLYSEKYACPDCGISMPPLAPRVFSFNNPYGACPQ
                     CNGLGSRMHFAPDLVVPDPGLSLREGAIAPWANRSSLYYFNLLDALSSHYKFDLNMPF
                     DKLAEKIRHVLLYGSGKEAIRFHHDRDNMRYFAERPFEGVIPQLERRWKETASQVIRN
                     DLARFIDLRPCEACGGARLKKESLAVTVGGKNIYELCLMSIRECFGFFEALRLTGLEE
                     QIVERVLKEIKNRLSFLLNVGMDYLSLARSTATLSGGESQRIRLATQIGSGLMGVLYV
                     LDEPTVGLHQRDNLRLIDTLKKLRDMGNTVLVVEHDADMMLACDHIVDMGPGAGTQGG
                     EVIFQGSPAEVLQSETSLTGLYLSGRKSIGLPAWRKVRGRHIVLEGASENNLKDIDIR
                     IPTGVLTAVTGVSGSGKSTMVIETLCKVMARRLNQHSGAMARIRRVKDMGGIERVIMI
                     NQQPIGRTPRSNPVTYTGIFSFIRELFTGLPEARVRGYKPGRFSFNVKGGRCEACEGN
                     GLIKIEMHFLPDVYVTCDVCRGKRFNADTLDIKYKDKSIADVLDLTVSQALDFFANIP
                     SIRSHLQLLADVGLGYIRLGQSATTLSGGEAQRIKLARELGKKLNRNTLYILDEPTIG
                     LHFADIQKLLDVLMRLVDMGNTMVVIEHNLDVIKSADHIIDLGPEGGPGGGRIIASGT
                     VEEVARNPRSFTGAFLKKALDDLRKRRAEEA"
     gene            complement(4270..5247)
                     /locus_tag="PLQ77_05420"
     CDS             complement(4270..5247)
                     /locus_tag="PLQ77_05420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL10150.1"
                     /translation="MKIYRKGLAVILAVLLISAPAAAELADRIVAVVNGEVITLSDLN
                     RAFEPYSDQIEEKYKGPDKGAFLQKNREAFLQRLIDQMLIEQEAKKPGAGVADVKDEE
                     VMGVIRDMLVKNQMTMPAYVKKLAEEGQTLEGVKKEIRGQMLRMRLLRREVQSRILVT
                     DEEIGEYYDKHRQEYEGREAVHIRLIFLPAPRGADRAARDGVGAEANRLRERILKGER
                     FEVIAARYSKGPAAAEGGDIGFVERGVMMPEVEKAAFDLPVGEVSGVIDTEEGFQLLT
                     VVDRKGAGLKPLSVVRDEIKSKIEDEKVAKKYDEWMDGIRKKSFIDVRL"
     gene            complement(5267..6253)
                     /locus_tag="PLQ77_05425"
     CDS             complement(5267..6253)
                     /locus_tag="PLQ77_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPL10151.1"
                     /translation="MMKRTVWQNLVWQVRPLAPLAMLCALCLVSACGRQEAERPHVAT
                     VNGEKIYLEEYQARLGDRRGLFSLRALQNESGKRKRLEEEILETLITEKIILQRAREL
                     GLSVSQTELEERLLEIRKDYGENFLDLLARQNVRYEDWCEALRKEMLLEKLVAGDVHA
                     ALRVSEDEAEDFFHGNPGFCRSGEKVRAFQIVTPDLERAREVKGRLEKGEDFSELAKA
                     FSIGPEAVRGGDLGLIARETMPEPLDRTLFSLRPGKTGPIVKSAYGYHIFRIAERQPA
                     HARSFDQCRQDILDFLRAQKEEEAFRAWLNALKMKAVVKKETNVSGRGTPSS"
     gene            complement(6365..9913)
                     /gene="mfd"
                     /locus_tag="PLQ77_05430"
     CDS             complement(6365..9913)
                     /gene="mfd"
                     /locus_tag="PLQ77_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPL10152.1"
                     /translation="MRKIVDKKMVHDAPPVRELLEAIAKGAPAADARGLVPPARPFLT
                     ALLFRRLAAPLIVLCPTAKEAAAFARDLELFLGEGAALHYPPLDFLSVDMFALQREEE
                     MARLNVLAQLQVDSRRVVVTSPSACLQKVMPYAEFQQYLRILSVGDVLLMEEFPEKLA
                     AGGYRRMSLVENPGEFSLRGNILDVFPPGAAEPLRLEMAGDEIESIRRFDASSQRSGA
                     SVDAFVLGPAGEILLDPERLNRAVRNVKRRADDLDLPRDLRNRLSETLREQSATSLNP
                     IFLPLFYEDYDDPAGFGRRNLSNVFDYLPRRSLLVIDDPLAVEQAHQNVSAGIDKLLY
                     KAKASGRFYLERAGLYLDDEMLHERMDGFQQVRLCGLALDGPKSAAPAVSFDTRLDAP
                     VAGPLARDGKEESHLGRAAEKIQNRLAEGMPVFLMSPTPEDTARVRRLLSEYGLPAQM
                     LPAASPLLEALLNPAAALYLREGKISGSFVMGSPRLAFLSEEDIFVKKTPRRRVRPSR
                     EGYFLKSFGDLKEGDFVVHRDFGIGLYRGLKKIAVGAVENDFLVIEYLDGDKLYLPVN
                     ALEKIQRYLGPDGCTPKIEKMGGASWDAVKEKVKKSVRDVAEELVAIYAAREAMERKP
                     FLPPDRVYEEFCSTFEYEETPDQARAIEDIHADMDEVKPMDRLICGDAGFGKTEVALR
                     AAFRAAMDGKQTALLAPTTILAEQHFATFSRRLSDFPIRVEALNRFRSPAEIKKTLAD
                     LLRGRVDVVVGTHRLLQKDVAFKNLGLVIIDEEQRFGVAHKEKLKKMRTLVDVLTLSA
                     TPIPRTLHLSLAGIRDLSIINTPPADRRPIKTYVMEFDEDVIRTAVEAEISRGGQVFF
                     VHDRVRSIYGIANLVQRLVPRARVGVVHGQMKPAEIEKTMARFIRREFDVLVCTTIIG
                     AGLDIPSANTIFINRADRFGLSQLYQIRGRVGRGSEEASAYLLLPRGALLSRDALRRL
                     QAIKEFSEPGCGFRIAHNDLEIRGGGNLLGLSQAGHISAVGYELYTELMEKTVREIQG
                     LPAPVEEALPEIQMGISAFLPEDYVQDVHRRLVLYKRISLASGPEDLQDLRAELTECY
                     GPLPQTAENLLRVIGLRNLLAPLNARKMGFDGKYFYIYFSETSPIDPARITALARKKI
                     RDLRFTPDFRLFLPARPAGAPEILDQAEELLKMLAQ"
     gene            complement(9941..10846)
                     /gene="nadA"
                     /locus_tag="PLQ77_05435"
     CDS             complement(9941..10846)
                     /gene="nadA"
                     /locus_tag="PLQ77_05435"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPL10153.1"
                     /translation="MNEQQKKIRAMLADRKGILLVHYYARPEIQEIADVLGDSLALAQ
                     AAARSDARMIVFAGVHFMAQSAAMLSPEKIVLLPRLDAGCPLADMAGPDAVRKMRQDH
                     PDAVFVTYINSTAEVKAESDICCTSSNALKIIQALPPSKKAVMLPDGNLARHAAAATG
                     REIIAWEGCCPIHQALTGEDVAARRREHPRAVFAAHPECRPEVLALADFVGSTSAILR
                     FARETKAMEIIIGTEKGILTQLAKENPEKTFIPASDALFCDDMKKISLDDIERSLTTM
                     QPVITVPRQVSEAAKKALDKMLAVS"
     gene            11238..12473
                     /locus_tag="PLQ77_05440"
     CDS             11238..12473
                     /locus_tag="PLQ77_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPL10154.1"
                     /translation="MITVEKALHTVLAACRPMGLEKADLLKAAGRVIGEDVFAPRDIP
                     SAANSAMDGYAVRSGDTKGAHALKPVELKVVETIAAGSLPRRTVQAGQAARIMTGAVI
                     PKGADAVVRREDTEEKGKTVLMKIAAPRGLDIRFPGEDVRQGEKVIAAGSVLRPGHIG
                     MLAVLGKAFVAVYQRPRVAILSTGDELVDIETEPPPGKIVNSNTYSLAAQILACGGAP
                     VLLGIGSDDKEDLVKKFQVARRADVILSSGGVSVGDYDFVKDVMGDIGNAMHFWQVAM
                     RPGKPLAFGSIDGVPLFGLPGNPVSVMVSFEQFVRPFLLRMQGHTRIFRPTLTAVSAQ
                     EIKKSAGVKNFLRAVVEREKNRCTARITGEQGSGILKSMVEANAFIVLDEDVSHVKKG
                     ENVLVQLLEETLTGADVPA"
     gene            12531..>13217
                     /locus_tag="PLQ77_05445"
     CDS             12531..>13217
                     /locus_tag="PLQ77_05445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPL10155.1"
                     /translation="MRKLMVVVCVALIAVWGCSPEAPKTEDQKAFYALGVHLSKQLSV
                     FDLTPEELKYVQLGMSDAAGGKKLAAEPEANMQKLGELAQARMQKATEKQKALAKPFL
                     EKAAKEAGVKKTASGLIYKEIKAGTGAQPKATDLVKVHYVGTLIDGKEFDSSVKRGQP
                     AELPLSQVFPCWTEGVSMMKVGGKSKLICPSEIAYGDRGRPPVIPGGATLVFEVELLD
                     VKDAKSAARPS"
BASE COUNT         2528 a         4054 c         3887 g         2748 t
ORIGIN      
        1 ggaagccaac cggctttacc aatagaatag tcggtatttc agggatcact ggcgggtctg
       61 aagacccgcc ctacattttt ttacgcaggg cgtaccttca ggtgcgcctg acagggctgc
      121 gctcttttgt cgccgcgaag agccgtcggt ctcaggacag gaagccctgc cgtccctgcg
      181 tccggtagat gtcgaggcag gcatcgacca cgtttgcgtc gtaaaggatt ccgcggtggt
      241 cggtgatttc cttcatggcc gtgtcgatgt ccatggcggg gcggtacggc cggtgagagg
      301 agatggcttc aatcacatcg gccacgccga tgatccgcgc ttccaggagg atatcgtccc
      361 ccttcaatcc ctgcggatag ccggacccgt ccatccgttc gtggtgctgc aggatgatct
      421 tcgcaaccgg atagggaaat tcaatcgcct tgagaatatc gtaggcgttc cggcagtggg
      481 ttttgaccat ctccatttcc agggggctca atcgccccgg cttggtcaga atctcggacg
      541 ggacggcaat cttgccgatg tcatgaagct ggcccgcgat ccgaatggct tccacccggt
      601 ccacgggcag gagcagcttc tccgagatga cgcaggcgat tctggccacc tgctgttcgt
      661 ggccggccgt gtaagggtcc cggctttcca cgattctgga catggccagc accgtccctc
      721 ccagaatcat ccggattttt tccaggctgg accggagttc ttccaggctt tggttcaggt
      781 gccgggtttt ctgccgcact ttctcttcca gtcccttgtt gaggtccaga agctcggcat
      841 tctgtttttt gaccagctca tccagccggc ggttttcccg catcagatcc gccttgccca
      901 gcatctgccg gatcgtgtga agaaaatcct ccctgctcca gggcttggtc agataccggt
      961 gaatgcctcc gctgttgatc gcgtcgatga tggcgtccgt gtccgtgtag cccgtcagca
     1021 gcacccgcag ggcgtcggga ctgatatggc gggcctgggc gaaaaactgc acgcccgtca
     1081 tgccgggcat gtgctggtcg gaaatgatca gggaaacgtc cggggttttt ttcagaaggg
     1141 ccagcccttc ttccgcggag ccggccgtca gcaaatcata tccctcatcg gccagcatcg
     1201 ccgccatgat ctcgaggatg gcttcctcgt cgtcaatcag caggatcgaa tgccggtatt
     1261 ttaaaggcga caacgccatg gaattcactt gctttcgtct ccaaagaccc ttcgtttttt
     1321 ccggcccgtc ttttgcgcgc cggccgaaaa cgcctgctct atacctgaaa agcgcgccgt
     1381 acacaaaata aaaaaccggc cgttatccgg aaggcggcgc gttcatgctt cttcggcgcg
     1441 ccgcttccgc aggtcgtcga gcgctttctt cagaaaagcg ccggtgaaag accgcgggtt
     1501 tcgcgccacc tcttccaccg tgccggaggc gataatccgc ccgccgcccg gaccgccttc
     1561 gggccccagg tcgatgatat gatccgcgga cttgatgaca tccaggttgt gttcgatcac
     1621 caccatggtg ttgcccatgt ccaccaggcg catcagcacg tccaggagct tctgaatgtc
     1681 cgcgaagtgc aggccgatgg tgggctcgtc cagaatgtac agggtattcc ggttcagctt
     1741 tttccccagc tccctcgcca gcttgatgcg ctgggcttcg ccgccggaga gcgttgtggc
     1801 cgactggccg agacggatgt atccgaggcc gacatccgcc agcagttgga gatgcgagcg
     1861 aatggacggg atgttggcaa aaaaatccag cgcctgagaa acggtcagat ccagaacatc
     1921 ggcgatgctt ttatccttgt atttgatgtc cagcgtgtcc gcgttgaagc gcttgccccg
     1981 gcagacgtcg caggtgacgt aaacatccgg aaggaagtgc atttcaattt tgatgagccc
     2041 gtttccctcg caggcttcgc agcggccgcc cttgacgttg aagctgaagc gccccggctt
     2101 gtagccgcgg acgcgcgctt ccggaagtcc ggtgaacaat tcgcggataa aggaaaaaat
     2161 gccggtgtag gttacgggat tggaccgcgg cgttcgtccg atcggctgct gattgatcat
     2221 gatgacccgc tcgatgcccc ccatatcctt tacgcgccgg attctggcca tggcgccgct
     2281 gtgctggttc aggcggcgcg ccatcacctt gcagagcgtt tcgatcacca tcgtgctttt
     2341 gccggagccg gaaacgccgg ttacggcggt caggacgccc gtcggaatcc gaatgtcgat
     2401 gtctttgagg ttgttttccg acgcgccttc cagaacgatg tggcggcccc tgaccttgcg
     2461 ccaggcggga agcccgatgg atttccggcc ggagaggtag agcccggtga gcgacgtttc
     2521 gctttgcagc acttcggcgg ggctgccctg aaaaatcacc tcgccgccct gggtgcccgc
     2581 gcccggcccc atatcgacga tgtggtcgca ggccagcatc atgtcggcgt cgtgctccac
     2641 caccagcacc gtgttgccca tgtcgcgcag cttcttgagc gtgtcgatca ggcgcaggtt
     2701 gtcccgctgg tgcaggccga cggtcggttc gtccagaacg tacagcacgc ccatcagccc
     2761 ggagccgatc tgcgtggcca gccggatgcg ctggctctct ccgccggaca gcgtggcggt
     2821 ggaccgcgcc aggctcagat aatccatgcc gacgttgagc agaaaggaaa gccggttttt
     2881 gatttccttc aaaacgcgct cgacgatttg ctcttccagg ccggtaagac ggagggcttc
     2941 aaaaaaaccg aagcactcgc ggatggacat caggcacagc tcgtaaatgt ttttgccgcc
     3001 cacggtcacg gccaggcttt cctttttcag ccgcgcgccg ccgcaggctt cgcaggggcg
     3061 cagatcaata aagcgcgcca ggtcgttgcg gatcacctgc gaggccgttt ctttccagcg
     3121 ccgctcaagc tgcggaatca cgccttcaaa agggcgctcg gcgaagtagc gcatgttgtc
     3181 gcggtcgtgg tgaaaacgga tggcttcctt tcccgacccg tacaggagga catggcggat
     3241 tttttccgcg agtttgtcga agggcatgtt caggtcgaat ttataatgcg aggaaagcgc
     3301 gtcgagcagg ttaaagtagt acagcgaact gcggttggcc cagggcgcga tggcgccttc
     3361 gcgaagcgac aggcccggat cgggcaccac caggtccggc gcgaagtgca tccgcgatcc
     3421 caggccgttg cactgggggc aggcaccgta gggattgttg aatgaaaaca cgcggggggc
     3481 gagaggcggc atgctgatgc cgcagtccgg acaggcgtat ttttcggaat acagggtttc
     3541 ccggccctcg gcggcggcaa tgcggaccag tccgtcgctc ttgtgggcgg caatctccag
     3601 ggaatcccga agccgctttc ggatgccgtc tttcaggacc aggcggtcca cgatcaggtc
     3661 gatgtcgtgg cgtttggttt tggcaaggac gatgtcctcc gccaggtcgc ggatttctcc
     3721 gtcgatgcgg acccgcgcaa aaccttcctt ctgaagcttt ttcaattctt tggcaaactc
     3781 gccttttttg ccccgcacca gcggcgacaa aatgctgaag cgcgtccctt cgggcaggtc
     3841 gagcacgctg tgcagaatgc tgtcgatggt ctgcgagtga atctcccgcc cgcagccgct
     3901 gcagtggcat acgccgatgc cggcgaacag cagccggaaa taatcgtaga tttccgtgac
     3961 cgtgcccacg gtggagcgag gattgtggcc ggccgcccgc tgttcgatgg cgatggcggg
     4021 cgaaaggcct tcgatggatt ccacctccgg cttgtccatc tggccgatga actggcgcgc
     4081 gtaggtcgaa agcgattcga cgtagcggcg ctggccctcg gcgtagatgg tgtcaaaggc
     4141 cagggaggat ttgccggagc cggaaacgcc ggtgatcacc accagccggt cgcggggaat
     4201 gtccagagaa acctttttga gattgtgctg gcaggcgccc ttgattctta tgcaatccat
     4261 tgccgtttcc taaagccgga cgtcaatgaa ggattttttg cggatgccgt ccatccattc
     4321 gtcatatttt ttggcgacct tttcatcttc aatcttcgat ttgatttcat cgcgcaccac
     4381 ggacagaggc ttcagccccg ctccttttct gtccacgacc gtcaggagct gaaatccctc
     4441 ttccgtatcg atgacgccgc tgacctcccc cacgggcagg tcaaaggccg ccttttcgac
     4501 ttccggcatc atgacgcccc gttccacaaa gccgatgtcg ccgccttcgg ccgcggccgg
     4561 ccccttcgaa tagcgggcgg cgatgacttc aaaccgctcg cccttcagga ttcgctcgcg
     4621 aagccggttc gcctcggccc cgaccccgtc gcgcgccgcg cggtccgccc cccggggcgc
     4681 cggcagaaaa atcagccgga tatgcaccgc ttcccggcct tcgtattcct gccggtgctt
     4741 gtcgtagtat tcgccgattt cctcatccgt caccagaatc cgggactgca cctcccggcg
     4801 cagaagacgc atgcgcagca tctgtccccg gatttccttt ttgacgcctt ccagcgtctg
     4861 gccttcctcg gccagctttt ttacataggc cggcatcgtc atctggtttt tcaccagcat
     4921 gtcccgaatc acgcccatga cttcttcatc cttgacgtcc gcgacgccgg cgcccggttt
     4981 tttcgcctcc tgctcgatca gcatctgatc gatcaggcgc tgcagaaaag cttcccggtt
     5041 cttctggaga aaggccccct tgtccgggcc tttgtacttt tcctcgatct ggtcggaata
     5101 gggctcgaaa gcccggttga gatcggaaag cgtgatcact tctccgttca cgacggcgac
     5161 gattcggtcc gccaattccg cggccgccgg cgcactgatc agcaaaacgg cgagaatgac
     5221 cgccaaacct tttctgtaaa tcttcacttg attccccctg ttcaaatcat gatgacggcg
     5281 tgccgcgccc ggaaacattc gtttcctttt tcacgacggc tttcatcttc aacgcgttca
     5341 gccaggccct gaacgcctct tcttcctttt gcgcccggag aaaatccaga atatcctgcc
     5401 ggcattggtc gaaggaacgg gcatgggccg gctgacgctc cgcaatcctg aaaatatggt
     5461 atccgtaggc gcttttgaca atggggcccg ttttgccggg ccgaagggaa aagagcgtcc
     5521 tgtccagcgg ctccggcatg gtttcccggg cgatcaggcc caggtctccg ccgcggaccg
     5581 cttccggccc gatggaaaac gctttggcca gttcggaaaa gtcttcgccc ttttccagtc
     5641 ttcccttgac ttcccgcgcc cgttccaggt cgggcgtgac aatctggaac gcccggacct
     5701 tttccccgga gcggcagaaa ccgggattcc cgtgaaaaaa atcctccgct tcgtcttccg
     5761 aaacgcgcag ggccgcatga acgtccccgg ccacaagctt ttcgagcagc atctcttttc
     5821 tcagggcttc gcaccagtct tcatagcgca cattttgccg ggcgagcaag tccaaaaaat
     5881 tctctccgta atctttgcgg atttccagaa gcctctcttc cagctccgtt tggctgaccg
     5941 acaggcccag ctcgcgcgcc cgctgcagaa tgattttttc cgtaatgagc gtctccagta
     6001 tttcctcttc caggcgcttg cgtttgccgg attcgttctg aagggcccga agcgaaaaca
     6061 gccccctgcg gtcgccgagc ctcgcctggt attcctccag gtaaattttt tcgccgttga
     6121 ccgtcgcgac atgcggacgc tcggcttcct gccgtccgca cgcggaaacg aggcacaggg
     6181 cgcaaagcat tgccagcggc gcaagcggcc ttacttgcca aaccagattt tgccaaaccg
     6241 tccgcttcat catgcgccgt ttccccgaga cgtttgcacc actacagaaa accaagaatt
     6301 ttgtcattcc cgcgcgcctg attcaccgcg ccggaaaacg cctttatgca tatcccgata
     6361 aaggctattg ggcaagcatc tttagaagct cctccgcctg gtccaaaatc tccggcgcac
     6421 cggccggacg ggccggcaga aacagcctga aatcaggggt aaagcgcagg tccctgattt
     6481 tctttcgggc cagcgcggtg attctggccg gatcaatcgg ggaggtttcg ctgaaataaa
     6541 tataaaaata ttttccgtcg aaacccattt tcctggcgtt gagcggcgcg agaagattgc
     6601 gcaggccgat cacgcgcagc agattttccg ccgtctgcgg cagggggccg tagcattccg
     6661 tcagttcggc acggaggtcc tgaaggtctt cggggccgga cgcaagcgat attctcttgt
     6721 acaggaccag gcgccggtga acgtcctgca cgtagtcttc cggcagaaag gcggaaattc
     6781 ccatctggat ctccggaaga gcttcttcga ccggcgccgg aaggccctgg atttcccgca
     6841 cggtcttttc catcagttcg gtgtagagct catacccgac ggcggagatg tgccctgcct
     6901 gcgaaaggcc cagcagattg ccgcctccgc ggatttccag atcgttgtgg gcgatgcgga
     6961 acccgcagcc cggctcggaa aactccttga tggcctgaag cctgcgcagg gcgtcccgcg
     7021 agagcagcgc cccgcggggc aggagcagat aggccgaagc ctcctcgctg ccgcgcccca
     7081 cgcggccgcg aatctggtaa agctgggaca ggccgaagcg gtcggcgcgg ttgatgaaaa
     7141 tcgtattggc ggaaggaatg tccagccccg ctccgatgat cgtcgtgcag accaggacgt
     7201 cgaattcccg ccggataaac cgcgccatgg ttttttcgat ttccgcgggc ttcatctggc
     7261 cgtgcaccac gcctacgcgc gcgcggggaa ccagccgctg caccaaattt gcgatcccgt
     7321 aaatggagcg cacccggtca tggacaaaaa acacctggcc gccccgggaa atctccgcct
     7381 cgacggcggt ccgaatgacg tcctcgtcaa attccatcac gtaggtcttg atgggccgcc
     7441 ggtccgcagg cggcgtgttg atgatggaca ggtcgcggat gccggcaagc gacaggtgca
     7501 gcgtgcgcgg aatcggcgtg gccgagagcg tcagtacatc caccagcgtt cgcattttct
     7561 tgagcttttc cttgtgcgcc acgccgaagc gctgttcctc gtcgatgatc accagcccca
     7621 gatttttaaa cgccacatct ttctgcagca ggcggtgcgt tccgacgacg acatcgaccc
     7681 ttcccctgag aaggtccgcc agcgtttttt tgatttcggc ggggcttcga aaacggttga
     7741 gcgcctccac gcgaatggga aaatccgaca gccgccgcga aaatgtcgcg aaatgctgct
     7801 cggccagaat cgtggtcggc gccagaagcg ccgtctgctt gccgtccatc gccgcccgga
     7861 acgccgcgcg cagcgcgact tccgttttgc cgaaccccgc gtcgccgcag atcagccggt
     7921 ccatcggttt gacttcgtcc atgtcggcgt gaatgtcttc gatggccctg gcctggtcgg
     7981 gggtctcctc gtactcaaac gtcgagcaga attcttcata gacgcggtcg ggcggcaaga
     8041 agggttttcg ctccatcgcc tcgcgggcgg cgtaaatcgc caccagctcc tcggccacgt
     8101 cgcggacgga ctttttgacc ttttccttga ccgcgtccca ggaggcgccg cccatctttt
     8161 cgattttcgg cgtgcagccg tccggcccga ggtagcgctg aatcttttcc agggcgttca
     8221 cgggcaggta gagcttgtcg ccgtccaggt actcgatcac cagaaaatca ttttccactg
     8281 cgcccacggc aatttttttc aggccccggt acagcccgat gccgaaatcc cggtggacga
     8341 cgaaatcccc ctctttcaga tcgccgaagg attttaaaaa ataaccttcg cggctgggcc
     8401 ggacgcggcg gcggggcgtt tttttgacaa aaatatcttc ttcgctcaga aacgcgaggc
     8461 gggggcttcc cattacgaag cttccggata ttttcccttc ccgcaggtac agagcggcgg
     8521 cgggattcag gagcgcctcc aaaagcgggc ttgccgcggg cagcatttgc gcgggaaggc
     8581 cgtactccga aagcaggcgc cggacgcggg ccgtgtcttc cggcgtgggg gacatcagga
     8641 acaccggcat cccctccgcc agccggtttt gaatcttttc cgccgcccgg ccgaggtggg
     8701 actcctcctt tccatcgcgg gccaaaggcc cggcaacggg agcgtccagc cgcgtatcga
     8761 agctcaccgc gggggcggcg ctcttcgggc cgtccagcgc caggccgcag agcctaacct
     8821 gttgaaaccc gtccattcgt tcatggagca tctcgtcgtc cagatacagg ccggcgcgct
     8881 ccagataaaa ccggccggac gctttcgcct tgtacaggag cttgtcgatg ccggccgaga
     8941 cgttttgatg cgcctgctcc accgccagcg gatcgtcgat cacgaggagc gaacgcctcg
     9001 gcaggtagtc aaagacattg gaaagattcc gccggccgaa acccgccggg tcgtcgtagt
     9061 cttcgtaaaa cagcggcagg aatatcggat tgagagacgt cgcagactgc tcccgaagcg
     9121 tttcggaaag cctgtttcgc aaatcgcgcg gaaggtccag atcgtctgcg cgtcgcttga
     9181 cattgcgcac ggcgcgattg agcctttcgg ggtcgaggag aatttcgccg gccgggccca
     9241 gaacaaacgc gtccacggac gcgcccgagc gctgggacga ggcgtcaaag cggcggatgg
     9301 attcgatctc gtcgcccgcc atttccaggc gcaggggctc cgcggcgccg ggcggaaaaa
     9361 catccagaat attgccgcgc aggctgaact ccccgggatt ttccaccagc gacatccgcc
     9421 ggtagccgcc cgcggcaagc ttttcgggaa actcctccat cagcagcaca tcgcctacgg
     9481 aaagaattct cagatactgc tgaaactcgg catacggcat gactttctgc aggcaggccg
     9541 aaggcgaggt gacgacgacc ctgcggctgt ccacctgcag ttgcgcaagc acattgaggc
     9601 gggccatttc ctcttcccgc tgcagggcga acatgtccac ggaaagaaaa tcgaggggcg
     9661 gatagtgcag cgcggcccct tcccccagaa aaagctccag atcgcgggca aaggcggcgg
     9721 cttcctttgc cgtggggcac aagacgatca gcggcgcggc gagccgccgg aacagcagcg
     9781 cggtcagaaa cgggcgggcc gggggaacca gtccccgcgc atccgcggcc ggcgcgcctt
     9841 tggcgatcgc ctccagcagc tcccgcacgg gcggggcgtc gtgaaccatt tttttatcga
     9901 cgatttttct cacggaatgt catccaggcg aatcggtcgg tcaggaaacg gccagcattt
     9961 tatccagggc ttttttcgcg gcctcgctca cttgccgggg aaccgtgatc accggctgca
    10021 tcgtcgtgag cgaccgctcg atatcgtcca gcgatatttt cttcatgtcg tcgcagaaaa
    10081 gcgcgtccga ggcgggaata aaggtttttt ccggattctc tttggcgagt tgggtcaaaa
    10141 tgcctttttc cgttccgata atgatttcca tggcttttgt ttcccgggca aaccgaagga
    10201 tggccgacgt ggaccccacg aaatcggcca gggccagcac ctccgggcgg cactccgggt
    10261 gcgcggcaaa caccgcccgc ggatgctccc gcctgcgggc cgcgacgtct tcccccgtca
    10321 gcgcctggtg aatcgggcag cagccctccc acgcgataat ttcccggccc gtggcggccg
    10381 ccgcgtggcg ggccagattg ccgtcgggca gcatcaccgc ttttttcgat ggcggcaggg
    10441 cttgaatgat tttaagcgcg ttggacgaag tgcagcagat gtcgctttcg gccttcactt
    10501 ccgccgtgga gttgatgtac gtcacgaaaa cggcgtccgg atgatcctgc ctcatttttc
    10561 tcaccgcatc cggaccggcc atgtccgcca gcgggcagcc cgcgtccagg cgcggcagca
    10621 gcacgatttt ttccggggaa agcattgccg cgctctgcgc catgaagtgc acgcccgcga
    10681 aaacgatcat cctggcgtcg gagcgcgccg ctgcctgggc cagcgccagc gaatccccca
    10741 gcacgtcggc tatctcctga atttcgggcc gcgcatagta gtggaccagc aaaatgccct
    10801 tccggtcggc cagcatcgcg cgaattttct tctgctgttc gttcattctg ttccctttct
    10861 tttcaacggc tcttccctag cgcaaatgcc ggtgaaattc aactggcatt agtggatggt
    10921 gaatggtgga tagtggatag aaaaaaggtg tgcgtgccgc ttgtgacctt caggttatca
    10981 ggcgcactta tagcgggtct aaagacccgc actacagaaa aaaattattt tgtcaccccg
    11041 agcctagtgt tgtgtcctga gcttattgaa gggatcacca tgaaatcttt tcactcattg
    11101 cccattgatc gtcacttctt ttaccgttca ctatccacca ttcaccattc accactcttt
    11161 cccctgccct cgctttttga ttgacaaccc aaccgtctct gataaagtgt cccgctgaaa
    11221 aaggctggag aaacgaaatg attaccgtag aaaaagctct tcataccgtc cttgccgcct
    11281 gccggcccat gggattggaa aaagccgatc ttctgaaagc ggcgggccgg gtgatcggcg
    11341 aagacgtctt cgcgccccgc gacattcctt ccgccgccaa ctccgccatg gacggctatg
    11401 ccgttcggtc cggcgacacg aaaggcgcgc acgccttaaa acccgtcgaa ctcaaggtcg
    11461 ttgaaacgat cgccgcgggc agccttcccc gcagaacggt tcaggccgga caggcggcgc
    11521 gcatcatgac cggcgcggtg atccccaaag gcgcggacgc cgtggtccgc cgggaggaca
    11581 ccgaagaaaa aggcaaaacg gttctgatga aaatcgccgc gccccggggg ctggacatcc
    11641 ggtttcccgg cgaggatgtg cgtcagggag agaaagtcat cgcggcaggc agcgttttgc
    11701 ggccgggcca catcggcatg ctggccgttc tgggaaaggc gtttgtcgcg gtttaccaga
    11761 ggccgcgcgt ggcgatcctg tccaccggcg acgaactggt ggacattgaa accgaaccgc
    11821 cgcccggcaa aatcgtcaac tccaacacct attcgctggc cgctcagatt ctggcctgcg
    11881 gcggcgctcc ggttcttttg ggcatcggca gcgacgacaa ggaagatctg gtcaaaaaat
    11941 tccaggtggc gcggcgggcg gacgtcatcc tgtcttccgg cggcgtctcg gtcggcgatt
    12001 atgatttcgt caaggatgtg atgggcgaca tcggcaacgc catgcacttc tggcaggtgg
    12061 ccatgcgccc cggaaagccg ctggctttcg gctccatcga cggcgttccc ctcttcggcc
    12121 tgcccggcaa tcccgtatcc gtcatggttt cctttgaaca gtttgtacgc ccctttctgc
    12181 ttcgcatgca ggggcatacc cggattttcc gcccgacgct cactgccgta agcgcccagg
    12241 aaatcaaaaa gagcgccggc gtcaaaaatt ttctccgggc ggtcgttgaa agagaaaaaa
    12301 accgctgcac tgcgcgcatc accggcgagc aggggtccgg cattctgaag tccatggtgg
    12361 aagccaatgc ttttattgtt ttggacgaag acgtctccca tgtgaaaaag ggagaaaacg
    12421 tgttggttca acttcttgaa gaaacactga ccggggcgga tgttcccgcc tgaacctggc
    12481 gcccgatccc ggggcaggaa ttaaagaaag ggtaatagaa aggattaatt atgcgtaaat
    12541 taatggttgt ggtttgtgtc gccctgattg ccgtctgggg atgttcgccc gaagcgccga
    12601 aaacggaaga ccagaaggcc ttctacgcgc tgggggttca tctcagcaag cagctgtccg
    12661 tttttgatct gacgcccgaa gagctgaagt atgttcagtt gggaatgtcc gacgcggccg
    12721 gcggcaagaa gctggcggcg gaaccggaag ccaacatgca gaaactgggc gaactggccc
    12781 aggcccgcat gcagaaagcc acggaaaagc agaaagcgct ggccaagccg tttctggaaa
    12841 aggccgcgaa ggaagcgggc gtaaagaaaa ccgcgtcggg attgatctac aaggaaatca
    12901 aagcgggaac gggcgctcag ccaaaggcca cggacctcgt caaagtccac tacgtgggca
    12961 cgctgatcga cggcaaggaa tttgacagct ccgtcaagcg cggccagccc gcggaactgc
    13021 ctttaagcca ggtgttcccc tgctggacgg agggcgtcag catgatgaag gtgggcggaa
    13081 aatccaaact gatctgccct tcggagatcg cctacggcga ccggggccgt ccccccgtga
    13141 ttcccggcgg cgcgacgctg gtctttgaag tggagcttct ggacgtgaag gacgccaaat
    13201 ccgccgcaag gccgtcc
//
LOCUS       DAOPAF010000042        12350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5078_length_12350_cov_62.254006, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000042 DAOPAF010000000
VERSION     DAOPAF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5078_length_12350_cov_62.254006"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1928
                     /locus_tag="PLQ77_05450"
     CDS             93..1928
                     /locus_tag="PLQ77_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL10156.1"
                     /translation="MEFKYDVRDLKFILKEWLPTGEVLACDRYKDNFSMDDIDMLLNE
                     GCKVAREVVNPINAPGDKIGVKFDKGVVTPVPGFKEAYQFLQQNGWGSSSECIKVETG
                     MPLVLYKACAEMNTAACPALTSCIKLTSGAANLILRFGTKEDQDRFVPKMLDGSWQGT
                     MCLTEPSFGSDTGDIITRSYPTDDPRIWKIKGTKMFITAGDQGTCENTIHLVLARPEG
                     AAAGSPGIGLYVVPKYWVNEDATMGDFNDVATVGVEHKMGLHAQATALLNFGDDDNCR
                     GIMLGAAPDEKGFSKGLAMMFHMMNESRIGTGHNANTQGAAAYYFASQYAAERVQGRP
                     FGIKNAERVPIIQHEDIRRMLLDMKAHVEGIRAMIFRGFYYLDIESNSPDKEKAKKCS
                     SFAEILTPIVKCYGSEASLGVIAQAIQVLGGVGYTLDYPVEQYLRDSKILTIWEGTSF
                     IHANDLIGRKMRMKDGAPFAAWMQDIGNFIEANQNAEGFEKEMKSLSDAFAAAEEVRT
                     IYNAWWADMEHKRRLIPLYAQKALFVFAYVYVAMCLLDQALIAAKKSQDLKPGEGDHT
                     FYAGKIASARYYANNILPNVPMLAALIKSEEDTVLACPEEALVVS"
     gene            complement(2111..4243)
                     /gene="scpA"
                     /locus_tag="PLQ77_05455"
     CDS             complement(2111..4243)
                     /gene="scpA"
                     /locus_tag="PLQ77_05455"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPL10157.1"
                     /translation="MTAHQNMKEWKEAALRELKGKPLESLNWATPEGIVVKPIYTADD
                     LEGLDTVNTLSGLAPYLRGPSATMYANQPWTIRQYAGFATAKESNEFYRKNLAAGQKG
                     LSVAFDLATHRGYDSDHPRVAGDVGKAGVAIDSVEDMKILFDQIPLDKVSVSMTMNGA
                     VLPILAGYIVAAEEQGVAQKQLAGTIQNDILKEFLTRNTYIYPPRPSMRIVSDIIAYS
                     SKNMPRYNTVSISGYHIMEAGADSVLQTAFTLADGKEYIRAAIDGGLKIDDFAPRLSF
                     FFGVGMNFFMEIAMLRAARYLWHQIVGEFHPTNPRSQVLRTHVQTSGWSLTQQDPYNN
                     IIRTTLEALAAALGGTQSLHTNSFDEAVSLPTPLSSRIARNTQIVIQEESQICRVVDP
                     LGGSYYVEALTASIIREAKKIMDEIEELGGMARAIETGMPKMRIEESAARRQARIDQG
                     KDTIVGVNKYKIKEDAPIDVLDIPATVRDEQIARIKAIKAKRDNAAVVKALEKITHVA
                     EKGGNLLEASIEAVRLRATVGEVSDAMEKVFGRYVATTRVISGAYASEYGDDDVIRSL
                     RARTEKFLEKNGRRPRQLVTKMGQDGHDRGIKVVATAYADIGFDVDISPMFQTPEEAA
                     KMAIENDVHVVGVSSLAAGHKTLVPELIEQLKKMGGQDIIVVVGGVIPPADYGFLYEK
                     GVKGIFGPGTAVTDSADRVLRLLEEKYG"
     gene            4633..6027
                     /locus_tag="PLQ77_05460"
     CDS             4633..6027
                     /locus_tag="PLQ77_05460"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPL10158.1"
                     /translation="MKDSMIHPVRIQDNTFRDGHQSIHATRMKTEDMIPIAEEMDDCG
                     FWAMEVWGGATFDAMHRFLEEDPWRRPGILKKYIRKTPMSMLLRGQNLVGYRHYADDV
                     VRAFVDRACEIGIDIFRCFDALNDFRNLETCVERIKANGKHVEGTVCYSLTEGHLGGE
                     IYNLDFYVKKAGELEQMGADTLCIKDMAGLLSPYDAFNLFTELKKAVRVPLHLHTHYT
                     SGMGSMTCLKAVEAGVDIIDTCLSPYALRTSMPACEPIVAALQGSERDSGINLNKLLE
                     MSEHLEKIAPKYQHLLADQRLSIIDNGVLAHQIPGGMISNLTSQLKEMKALDRLGEIY
                     KEVVQTRKDMGYPPLVTPSSQIIGSQAVLNVLFGRYVRISNQLKDLVLGLYGRTPVPI
                     DPELTAKILKNYKRGQTPITGRPADHLPPEMEEAKKTIGDLARTDEDLLGWILFPQTM
                     EKYLRAKYGIDAPA"
     gene            6068..7735
                     /locus_tag="PLQ77_05465"
     CDS             6068..7735
                     /locus_tag="PLQ77_05465"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HPL10159.1"
                     /translation="MPTFDDRSGGFPRGLELLHDPGLNKGTAFTAAEREALGLQGLLP
                     PRISTQQQQVDHVIYNVRRKPNPLEKYLYLIGLQDRNEQLFYKALVDHLEELSPLVYT
                     PTVGLACQEYSHLYRRPRGITLTRYDRGRIKEALLNWPHRKVRVIVVTDGERILGLGD
                     LGSNGMGIPVGKLSLYTACAGIDPAACLPVMIDVGTNNNELWSDPQYIGLPEPRLEGE
                     AYDALIDEFMEAAEEAFPKVMIQLEDFGNRNAFRLLDRYRDRYCMFDDDIQGTAGVTL
                     AGLYSALRIIKGKITEQRIVFLGAGEAALGIGNLAASAMIRQGLAEEEALGRCWFVDS
                     KGLIVAGREDLNLHKRRFAHDFPFHRTLAEVVEAVRPTTLIGASGQPAAFGPEILEAM
                     ARLNERPIIFALSNPTSRAECTAEDAYRHTQGRAVFAGGSPFPKVEMNGQTFVPGQAN
                     NVYIFPGVGLGVMASDATRVTDEMFAAAAKCLDEQVSKDDLEQGRIFPSLARIRDVSA
                     AIALAVAKVAFAGKLTAMKEPSNLPGYIKSLMYDPVYSSYVPVKKHG"
     gene            7785..9050
                     /locus_tag="PLQ77_05470"
     CDS             7785..9050
                     /locus_tag="PLQ77_05470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL10160.1"
                     /translation="MENVVFISCARTPIGAYGGALREVPIYRLASIVLQAAVKKAGIE
                     PAQVDDVIMASAYQNGECANGARMAVLDADWPDTIPGGMIDRRCCSGLESVYYGALQI
                     QTGNADMVIAGGMESMSQGELYIPGDIKWGLGGKNHEKYGFMPRGHGALAMWGIPFYD
                     RIQRGRVMSQPIWRYGELSSMMTWAEAAAKKEGISREEADRWALRSHQRAIAAQDAGK
                     FTDEIAPVLVGKDKSGQEIYFKTDEGPRRETTLEKLAKLKAVYKDGVCTAGNSSSEND
                     GAAVVILASEKKAKELGIKPIAYYRSCAVAATDPTLTYPAVPAAVNKALAKIGKAIND
                     MDLIEVQEAFAVQCLADSRLAGLSDEQMDAKVNINGSGISLGHPIGATGTMRLTTLLN
                     EMQRSNARFALETICGGGGQGICMVIERT"
     gene            9244..10401
                     /locus_tag="PLQ77_05475"
     CDS             9244..10401
                     /locus_tag="PLQ77_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL10161.1"
                     /translation="MDFALTEEQKMIRETARNFAEKEIAPHVEEDEKNHFWRREIFDK
                     MAELGFFGFCIDEKYGGNGMGFLEGALAIEQVARVHVAWRMAFNMQCWGPALTIQKFG
                     TEEQKERYIPKFVSGEYVGSFAMTEPDIGSDVASMKCRAEDKGDYYLVNGNKMWITNG
                     TVCNHGLLYVKTDKDAGANGVSCFIMDYSLPGITRKPIHDKVGLWASDTAEITFEDVK
                     VPKNLLLGKLNKGFQICMTQLNSTRLGCSAGALGLSGAILDASAKYATERCQFGKQIG
                     RYQLIQQQVADMKVGHDTLAYLVYRSGWLKDKGLPNVMETSMSKLYGAKVVVHDANEC
                     MKLYGSFGYSDEYPCGRFLRDSKQFETLEGTSNMHTMIVANAVMGFAPNRA"
     gene            10612..>12350
                     /locus_tag="PLQ77_05480"
     CDS             10612..>12350
                     /locus_tag="PLQ77_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPL10162.1"
                     /translation="MKQYFEKMDPLGKPLSAKQVESMQENCAKIEEIMKTIDAEVERI
                     KNVGVSLQTLHDRGEMSVWERIEHLVDSGTFCPLHSIFDPENEESGSTGVVDGLARIG
                     GKWCALIGFNNKWIAGAWIAGQPDNNLRVTDIARILHIPLVWLVNCSGVKLPEQEKMY
                     ANRRGQGTCFFRHAELEQMGIPVIAGIYGTNPAGGGYQSISPTILLAHKKANMAVGGG
                     GIVSGMSPKGFFDEEGAEELINATRHFKSVPPGSVKVHHDVTGFFRAVFDEETQVLDA
                     IKDYMDDLPAYHPRFFRVAAPAEPAYPPEEIASIIPVNKKRVYDFEQVLARLVDGSEH
                     LEFRPGFGPEMYCGLVKVDGYLMGVIGNRQGAFPNYPEYTNEYMGVGGKLYRQGLIKM
                     SEFVTLCSRDKVPMVWFQDTSGIDVGDIAEKAELLGLGQSLIYSIENSKLPMMLVVLR
                     KGTAAAHYVLGGPTANNNNAFSLGTATTEINVMHGETAAAASYARRLVKEKDAGRPLG
                     PIIDKMNEMVKHYDDTSGPMFCAKKGFVDEIVRYHELRNYLVGFANCCYQNPRSICPQ
                     HQMILPRIIRSQIV"
BASE COUNT         2757 a         3568 c         3655 g         2370 t
ORIGIN      
        1 aaatatcggg tatgggaaaa agtaaaaaac aggcaatggg ctataggcga tgggccatag
       61 gctgaaggaa ataaatttga ggagacgcaa acatggaatt taaatatgac gttcgggatt
      121 tgaaatttat tttaaaggag tggctgccga cgggggaggt cctggcctgc gatcggtata
      181 aagacaattt ctcgatggac gacatcgaca tgcttctcaa cgaaggctgc aaggtcgccc
      241 gggaagtcgt caaccccatc aacgcgccgg gtgataaaat cggcgtgaaa tttgataagg
      301 gcgttgtgac gccggttccc gggtttaagg aagcctacca gttcctgcag caaaacggct
      361 ggggctcgtc gagcgagtgc atcaaagtgg aaaccggcat gccgcttgtc ctttacaaag
      421 catgcgccga aatgaacacc gccgcctgcc ccgcgctgac ctcctgcatc aaactgactt
      481 ccggcgcggc caatctgatt ttgcgcttcg gcacaaagga ggatcaggac cgtttcgttc
      541 ccaaaatgct tgacggctcc tggcagggca cgatgtgcct gaccgaaccc agcttcggct
      601 cggacacggg cgacatcatc acccgctcct atccgaccga cgacccgcgc atctggaaga
      661 tcaagggcac caagatgttc atcaccgccg gcgatcaggg cacctgcgaa aatacgattc
      721 atctggtgct ggcgcgtccc gaaggcgctg ctgccggttc tcccggcatc ggactttatg
      781 ttgttcccaa atactgggta aatgaagacg caaccatggg tgatttcaat gacgtcgcca
      841 ccgtcggcgt cgagcacaaa atggggctgc acgcccaggc cacggcgctt ttgaacttcg
      901 gcgacgacga caactgccgc ggcattatgc tgggcgccgc gcccgacgaa aagggctttt
      961 ccaagggact cgcgatgatg ttccacatga tgaacgaatc gcgcatcggc acgggacaca
     1021 acgccaacac gcagggcgcg gccgcttact actttgcgtc acagtatgcc gccgagcgcg
     1081 ttcagggaag accctttggg attaaaaacg ccgaacgggt tcccatcatc cagcacgaag
     1141 acattcgccg aatgctgctg gacatgaagg cgcatgtgga aggcatccgc gccatgatct
     1201 tccggggatt ttactatctg gacattgaaa gcaactcccc cgataaagaa aaggccaaaa
     1261 aatgctcttc ttttgctgaa atcctcacac ccatcgtcaa atgctacggc tccgaggctt
     1321 cgctgggcgt catcgcgcag gcaattcagg tgctgggcgg cgtgggctac acgctggact
     1381 atccggtgga gcaatatctg cgcgattcca aaatcctcac gatctgggag ggaacgtcgt
     1441 tcatccatgc caacgatctc atcggccgga aaatgcgcat gaaggacgga gcgccctttg
     1501 ccgcatggat gcaggatatc ggaaatttca tcgaggccaa ccaaaatgct gaagggtttg
     1561 aaaaagaaat gaaaagcctc tccgacgcct ttgccgccgc cgaagaagtc cggacgattt
     1621 acaacgcctg gtgggccgac atggagcaca agcgccggtt gattccgctt tacgcccaga
     1681 aggcgctgtt tgtgtttgcg tacgtctatg tggcgatgtg cctcctcgat caggccctca
     1741 tcgcggcaaa gaagtcacag gatttgaaac ccggcgaggg agaccatact ttctacgcgg
     1801 gcaagatcgc ttcggccagg tattacgcca acaacattct gccgaatgtc ccaatgctgg
     1861 cggcgctgat taaaagtgaa gaagatacgg tgctcgcctg tcccgaggag gcgctggtcg
     1921 tgagctagga ccggctgcaa tgaatcagca aagtgtgatg cagtccaaaa atccagagtc
     1981 gcagggaacg gtcgcgaccg ttccctgcga ctcgatatcg aagggcaagc tgggaaggcg
     2041 cagaaagact tagagaggag aaatttgccg gtttttccgg gcggccgtct tcacggccgc
     2101 ccgtttgttt ttatccgtat ttttcttcca gaagccgcag aacccggtcc gccgaatccg
     2161 tgacggccgt gccggggccg aagatgcctt tgacgccttt ctcatagagg aagccgtaat
     2221 ccgcgggcgg aatgacgccg cccaccacga cgatgatgtc ctgaccgccc atcttcttca
     2281 actgttcgat gagctccgga accagcgtct tgtggcccgc cgcgaggctc gagacgccca
     2341 cgacatgcac gtcgttttca atggccattt tggccgcttc ctccggcgtc tgaaacatcg
     2401 gactgatgtc cacgtcaaag ccgatatccg cgtaggccgt ggccaccacc ttgatccccc
     2461 ggtcgtggcc gtcctgtccc attttggtca caagctggcg ggggcggcgt ccgttctttt
     2521 ccaggaattt ctccgtccgg gcccgaagcg accggatcac atcgtcgtcg ccgtattcgg
     2581 aggcataagc gccggagatc acccgcgtgg tggcgacata ccggccgaag actttttcca
     2641 tggcgtcgga aacctctccc accgtggccc gcagccgcac cgcttcaatt gaggcttcca
     2701 gaaggttccc gcccttttcg gcgacgtgcg taattttctc cagggccttg accacggcgg
     2761 cattgtcgcg cttcgctttg atcgctttga ttctggcaat ctgttcatcg cgtaccgtgg
     2821 cgggaatgtc cagaacgtct atcggggcat cttccttgat tttgtatttg tttacgccga
     2881 cgatggtgtc ttttccctgg tcgatgcggg cctgccgccg ggccgccgac tcctcaatgc
     2941 gcatcttggg catgccggtt tcaatggccc gggccatgcc gcccagctct tcgatttcgt
     3001 ccatgatctt tttggcttcc cgaatgatgg aggccgtgag cgcctccacg tagtaggagc
     3061 cgcccagggg atcgactacg cggcagatct gcgattcctc ctgaatgacg atctgcgtgt
     3121 tgcgggcaat gcgtgaagac agaggcgtgg gcaggctcac cgcctcgtcg aacgagttgg
     3181 tgtgcagcga ctgggtgccg cccagcgccg cggccagcgc ctccagcgtg gtgcggatga
     3241 tgttgttgta cggatcctgc tgggtgaggc tccagccgga cgtctgcaca tgcgtgcgca
     3301 gaacctgcga gcgcgggttg gtcggatgga actcgcccac gatctggtgc cacagatacc
     3361 tcgccgcccg cagcatggcg atttccatga aaaagttcat gcccacgccg aagaagaacg
     3421 acaggcgcgg agcgaaatca tcgattttca ggcccccgtc gatggcggcg cggatgtatt
     3481 ccttgccgtc ggccagcgtg aaagccgtct gcaggaccga gtccgccccc gcttccatga
     3541 tgtgatagcc gctgatcgac acggtgttgt agcgcggcat gtttttcgac gagtaggcga
     3601 tgatgtcgga aacgatccgc atggagggcc ggggcggata gatgtaggta ttgcgcgtca
     3661 gaaattcctt gagaatgtcg ttctgaatgg tgccggcgag ctgcttctgc gccacgccct
     3721 gctcttcggc ggccacgatg tatcccgcca gaatcggcag gacggcgccg ttcatcgtca
     3781 tggaaacgga aaccttgtcg agcggaattt ggtcgaagag aattttcatg tcctcgacgg
     3841 agtcgatggc cacgccggcc ttgccgacgt cgcccgccac gcggggatga tcggagtcgt
     3901 atccccggtg agtggccagg tcgaaggcca cagacaggcc cttttgtccc gcggcgagat
     3961 tcttccggta gaattcattg gattccttgg ccgtggcgaa acccgcgtac tggcggatcg
     4021 tccagggctg gttggcgtac atggtggccg aaggccccct cagatacggg gcaagaccgg
     4081 acagcgtgtt gacggtgtcc agcccttcca gatcatccgc cgtgtaaatc ggcttgacga
     4141 cgatgccctc cggcgtcgcc cagttcagag actccagggg ctttcccttc agctcccgga
     4201 gggcggcttc tttccattct ttcatgttct ggtgtgcggt cacgttgaaa cctccttcga
     4261 tataatgcgg gaagcggccg caaccgctcc ccgacggatt tttgcatcgg ccgcgcgcgg
     4321 gccatcctcg aaaacggcag cctgtttata aaatatacag ttcagaaaca agccgtatag
     4381 gtgatataca gcgtcccctc ccctgcaaac cgaaaggtgg cataccacat ttgcgcggca
     4441 atagcataat atctaatcaa acgcttcgcg gcaagctgcg gagaatcaac gctcgttccg
     4501 ccaaagcggg attgagaacc gaagaacgcc ggacatttac cgcgccgccc ttcattgaaa
     4561 gcgggaaacg gctggcatgc tatttgctgt aatctacggc gcggcatatt cgtacgcggc
     4621 aaggagtttc gcatgaaaga ttcaatgatc caccccgtca gaattcagga caacacattc
     4681 cgggacggcc atcagtccat acacgcgacc cggatgaaaa cagaggatat gatcccgatc
     4741 gccgaggaga tggacgactg cgggttctgg gccatggagg tgtggggcgg cgcgacgttt
     4801 gacgccatgc accgttttct ggaggaagac ccgtggaggc ggcccggaat tctgaaaaag
     4861 tacattcgga aaacgcccat gagcatgctg ctgcgcggac agaacctggt ggggtaccgc
     4921 cactacgcag acgacgtggt gcgggccttt gtcgatcggg cctgcgaaat cggcattgac
     4981 atcttccgct gcttcgacgc gctcaacgac tttcgcaacc tcgagacctg cgtggaaaga
     5041 atcaaggcca acggcaagca tgtggaagga accgtctgct actccctcac cgaaggccat
     5101 ctgggcgggg agatttacaa tcttgacttc tacgtaaaaa aagccggaga gctcgaacaa
     5161 atgggcgcgg acaccctctg catcaaggac atggccggtc tgctttctcc ctacgacgca
     5221 ttcaatttgt tcaccgagtt gaaaaaagcg gtccgggtcc ccctgcatct gcacacgcac
     5281 tataccagcg gcatgggcag catgacctgc ctgaaagcgg ttgaagccgg cgtggacatc
     5341 atcgacacct gcctgtctcc ctacgccctg agaacgtcca tgcccgcctg cgaacccatt
     5401 gtggcggcgc ttcagggatc ggagagggac tcgggaatca acctcaacaa gctcctggaa
     5461 atgagcgaac atctggaaaa gatcgccccc aaataccagc atctgctcgc ggatcagaga
     5521 ctttccatca tcgacaacgg cgtgctggcc catcagattc ccggcggcat gatttccaac
     5581 ctgaccagcc agctcaagga aatgaaggcc ctcgaccggc tgggtgaaat ctacaaagaa
     5641 gtcgtccaga cgcgaaaaga catgggctat ccgccgctgg tgacgccgtc ttcgcagatc
     5701 atcggctcgc aggcggtgct gaacgtcctg ttcgggcgct acgtccggat ttccaatcag
     5761 ctcaaagacc tcgtgctcgg tctctacggc agaacccccg tgccgattga tccggagctg
     5821 accgcgaaaa tactcaaaaa ctacaaacgc ggacagacgc cgatcaccgg caggccggca
     5881 gaccaccttc cccccgaaat ggaagaggcg aaaaaaacga tcggcgatct ggcgcgcacc
     5941 gacgaagatc tgctgggctg gatcctgttc cctcagacca tggaaaaata tctgcgggca
     6001 aaatacggaa tcgacgcgcc ggcataggcg gtcgcgggag agtatggata gggtgaggat
     6061 aaaaacgatg ccaactttcg atgaccgttc gggcggattt ccccgggggc tggaactgct
     6121 ccacgatcca ggcctgaaca aagggacggc ttttaccgcg gccgaacgcg aagcgctggg
     6181 cctgcagggt ctgctgccgc cgcgcatctc gacccagcag cagcaggtgg atcatgtgat
     6241 ctataacgtc cggcggaaac ccaatcctct ggaaaaatac ctatatctga tcggcctgca
     6301 ggaccgcaac gaacagcttt tttacaaagc gctcgtggac catctggaag aactgagccc
     6361 cctcgtttac acccccaccg tggggctggc ctgccaggaa tacagccatc tgtaccggcg
     6421 tccccgcggc attacgctga cccgctacga ccggggccgc atcaaagagg cgctgctcaa
     6481 ctggccgcac cggaaggtcc gggtcattgt cgtcacggac ggcgaaagaa tcctgggcct
     6541 gggcgacctg ggatccaacg gcatgggcat tcccgtggga aagctgtcgc tctacacggc
     6601 ctgcgcgggt attgacccgg cggcctgcct gcccgtcatg atcgatgtcg gcaccaacaa
     6661 taacgaattg tggagcgatc cgcagtacat cggcctgccg gaaccccggc tggagggaga
     6721 agcatacgat gcgctgatcg atgaattcat ggaagcggcc gaagaggcat tcccgaaggt
     6781 catgatccag ttggaagact tcggcaaccg gaatgctttc cgcctgctgg accgctaccg
     6841 cgaccggtac tgcatgttcg acgacgacat tcagggcacc gcgggcgtca cgctggccgg
     6901 gctctactcc gctttgcgca tcattaaggg gaaaattacc gaacagcgga ttgtgtttct
     6961 gggcgcggga gaagccgccc tgggcatcgg gaatctggcg gcctccgcaa tgatccggca
     7021 ggggcttgcc gaagaagaag ccctcggccg gtgctggttt gtcgattcca aagggctcat
     7081 cgtggccggc cgcgaggatc tgaaccttca caaacgccgc tttgcccatg attttccgtt
     7141 tcaccgcact ctcgcggaag tggtggaagc cgtccggccg acaaccctca tcggcgcgtc
     7201 ggggcagccc gccgcctttg ggccggaaat tctggaggcc atggcgcgtc tgaacgaacg
     7261 tccgattatc tttgcgcttt ccaatccgac ctcacgggcg gaatgcacgg ccgaggacgc
     7321 ttaccgccac acacaagggc gcgccgtttt tgccggcggc agtccgtttc cgaaggtgga
     7381 gatgaacggt caaacgtttg tgccgggtca ggccaacaac gtttacatct tccccggcgt
     7441 gggcctcggc gtcatggcct cggacgcgac ccgtgtgacg gacgaaatgt tcgccgcggc
     7501 ggccaaatgt ctggatgaac aggtatcaaa ggatgatctg gaacaaggaa gaatctttcc
     7561 gtcgcttgcg cgcatccgcg acgtttcggc cgccattgcg ctggccgtgg ccaaagtcgc
     7621 ctttgccgga aaactcaccg cgatgaagga accttccaat ttgccgggct atatcaagtc
     7681 gctgatgtat gacccggtct attcgtcgta tgtgccggta aaaaaacatg gttaaaaaaa
     7741 gccggcggag gtatgatcca caaaactgca cagggagaaa agcaatggaa aatgtcgtgt
     7801 ttatcagctg cgcgcggacg ccgatcggcg cgtacggcgg agcgcttcgg gaggttccga
     7861 tttaccgtct ggccagcatt gtcctgcagg cggccgtcaa aaaagcgggc attgaaccgg
     7921 cccaggtgga tgacgtcatc atggcgtccg cctatcagaa cggcgagtgc gccaacggcg
     7981 cgcgcatggc cgttctggac gccgactggc cggataccat ccccggcggc atgatcgacc
     8041 ggcgctgctg ctccggcctg gaaagcgttt attacggggc gttgcagatt caaaccggca
     8101 acgccgatat ggttatcgca ggcggcatgg aatcgatgag ccagggcgaa ctctacatcc
     8161 ccggagacat caaatggggg ctgggcggca agaaccatga gaaatacggc ttcatgccgc
     8221 gcggccacgg ggcgctcgcc atgtggggca ttccgtttta cgaccgcatt cagcgcggcc
     8281 gcgtcatgag ccagcccatc tggcgctacg gagaactgag ctccatgatg acctgggcgg
     8341 aagccgcagc caaaaaagaa ggcatctcgc gcgaagaagc ggaccgctgg gcgcttcgat
     8401 cgcatcagcg tgcgatcgcg gcgcaggatg cggggaaatt caccgatgaa atcgctccgg
     8461 tgcttgtagg caaggacaaa agcggccagg agatttattt caaaaccgat gaaggccccc
     8521 gccgcgaaac cacgctggag aagctggcca aactcaaagc cgtttacaag gacggcgtct
     8581 gcacggccgg caactcctct tcggaaaacg acggcgcggc cgtggtgatc ctcgcttctg
     8641 aaaagaaggc gaaggaactg ggcattaaac cgatcgccta ctaccggtcc tgcgcggttg
     8701 ccgccaccga cccgacgctc acgtacccgg ccgttccggc ggccgtcaac aaggcgctgg
     8761 caaagatcgg caaggccatc aacgacatgg acctgatcga agtgcaggag gcttttgccg
     8821 ttcagtgtct ggcggattcg cgtctggcgg gactttccga cgagcagatg gacgcgaagg
     8881 tcaacatcaa cggttcgggc atttcgctcg ggcaccctat cggcgccacg ggcacgatgc
     8941 ggctcaccac gctgctgaac gaaatgcagc ggagcaacgc ccgcttcgcc ctggagacga
     9001 tctgcggcgg cggaggacag gggatttgca tggtgatcga acgaacataa caccgtaagg
     9061 aacggtcgcg accgttcctt acatgaaaag gatcgaatgt agccaaccat taagaaagtg
     9121 gtgaatgtag ggcgggtctt cagacccgcc aacgcgcgcc tgaaggcacg cactggattc
     9181 cggccttcgc cggaatgacg aagatcagaa taagcactaa caacggccgg gaggaaaagg
     9241 gatatggatt ttgcattgac ggaagaacaa aaaatgattc gcgagacggc gcgcaatttt
     9301 gccgaaaagg aaatcgcgcc gcacgttgaa gaagacgaga aaaaccattt ctggcgcagg
     9361 gaaatttttg acaagatggc ggagctgggg tttttcggct tttgcatcga cgaaaaatac
     9421 ggcgggaacg gcatgggctt tctggaaggc gcgctcgcca tcgaacaggt ggcccgcgtg
     9481 catgtcgcct ggcgcatggc cttcaacatg cagtgctggg gcccggcgct caccattcag
     9541 aagttcggaa ccgaggaaca gaaagagcgc tacattccca aattcgtctc cggcgaatac
     9601 gtcggcagct ttgccatgac cgaaccggac atcggctcgg acgtcgcctc catgaaatgc
     9661 cgcgccgagg acaagggcga ctactacctg gtgaacggca acaagatgtg gatcaccaac
     9721 ggcacggttt gcaaccacgg cctgctgtac gtgaaaacag acaaggacgc gggcgcaaac
     9781 ggcgtgagct gcttcatcat ggactactcc ctgcccggca ttacgagaaa acccattcac
     9841 gacaaagtcg gcctgtgggc ctccgacacg gcggaaatca cgtttgaaga cgtcaaagtc
     9901 cccaaaaatc ttttgctggg aaaactgaac aagggattcc agatttgcat gacgcagctc
     9961 aactccaccc ggctgggctg ctccgcgggc gctcttgggc tttccggcgc cattctcgac
    10021 gcctccgcca aatacgccac ggagcgctgc cagttcggca agcaaatcgg ccgctatcag
    10081 ctcattcagc agcaggtggc ggacatgaaa gtcggccatg acacgcttgc ctatctggtt
    10141 taccgctcgg gctggctcaa ggacaaggga cttcccaacg tcatggaaac ctccatgtcc
    10201 aagctttacg gcgcaaaagt cgtcgtccac gacgccaatg agtgcatgaa gctctacggt
    10261 tcgttcggct attccgacga atatccctgc ggccgcttcc tgcgcgactc caaacagttc
    10321 gagacgctgg aaggcacgtc caacatgcac acgatgatcg tcgccaacgc cgtcatggga
    10381 tttgcgccca accgggcata acgagaaata tgccctgatg ccctctccct cgacgggaga
    10441 gggcatcagg gtgagggtga aaaatcttga tgtcattcga aagatttttt cctccggtct
    10501 tgccccatca atggggctgg aaatgacaaa cgtttaatca aacattgcat gtgtaccccc
    10561 tccccttcat cccctcccac aaggggaggg gatgtttata aggagaccgc catgaaacaa
    10621 tactttgaga aaatggatcc gctggggaaa cccctttccg ccaaacaggt cgaaagcatg
    10681 caggagaact gcgcgaagat cgaagagatc atgaaaacca tcgacgcgga agtcgagcgg
    10741 atcaaaaacg tgggcgtctc tttgcagacg ctgcacgacc ggggcgagat gagcgtctgg
    10801 gaacgcatcg agcacctcgt cgattcaggg accttctgcc cgctgcacag catcttcgat
    10861 ccggaaaacg aggaatcggg aagcaccggc gtcgtggacg gtctggcgcg cattggcggc
    10921 aaatggtgcg cgctcatcgg atttaacaac aagtggatcg cgggcgcgtg gattgccggc
    10981 cagccggaca acaacctgcg cgtgaccgac atcgccagga ttctgcacat tccgctggtg
    11041 tggctggtca actgctccgg cgtgaagctg ccggaacagg aaaaaatgta cgccaatcgc
    11101 cgggggcagg gcacctgctt tttccggcat gcggagctcg aacagatggg cattccggtc
    11161 atcgccggca tttacggcac caatccggcg ggcggcggct atcagtcgat cagcccgacg
    11221 attctgctgg cgcacaagaa ggccaacatg gccgtgggcg gcggcggaat cgtgagcggc
    11281 atgtccccca aagggttttt cgatgaagaa ggagcggaag agcttatcaa cgccacgcgg
    11341 cacttcaaat ccgttccgcc cgggagcgtg aaagtccacc acgacgtcac cggatttttc
    11401 cgggcggtct ttgacgagga aacacaggtc ctcgacgcca tcaaagacta tatggacgat
    11461 ctgcccgcct accatccgcg cttcttccgc gtggcggctc ccgccgaacc cgcttacccg
    11521 cctgaggaga tcgcctcgat cattccggtg aacaagaagc gcgtttacga tttcgagcag
    11581 gtgctggcga ggctggtgga cggctcagag cacctggagt tccgccccgg cttcggaccg
    11641 gaaatgtact gcggcctggt caaggtggac ggttatctga tgggtgtgat cggcaaccgt
    11701 cagggcgcct tccccaatta tcccgaatac accaacgagt acatgggcgt gggcggcaaa
    11761 ctctaccgcc aaggcctgat caagatgagc gagttcgtga cgctgtgctc gcgcgacaaa
    11821 gtgccgatgg tatggtttca ggacacgtcc ggcatcgacg tgggcgacat cgccgaaaag
    11881 gcggagctgc tgggtctggg acagtcgctg atttattcga ttgagaattc aaaactgccg
    11941 atgatgctgg tcgtcctgcg caaggggacg gcagccgcgc attatgttct gggaggcccc
    12001 acggccaaca acaacaatgc attttcgctg ggcaccgcca cgaccgaaat caacgtcatg
    12061 cacggggaaa ccgcggcggc ggcaagttac gcgcggaggc tggtgaagga gaaggacgcg
    12121 ggccggccgc tggggcccat tatcgacaag atgaatgaga tggtgaagca ctatgacgac
    12181 acgtcggggc cgatgttttg cgcgaagaag ggctttgtcg atgaaatcgt ccgctatcat
    12241 gaattgcgca actacctggt gggctttgcc aactgctgct accagaatcc ccggtccatc
    12301 tgtccgcagc accagatgat ccttccccgg atcatccgct cgcagatcgt
//
LOCUS       DAOPAF010000043        12253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5123_length_12253_cov_41.416298, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000043 DAOPAF010000000
VERSION     DAOPAF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_12253_cov_41.416298"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLQ77_05485"
     CDS             <1..745
                     /locus_tag="PLQ77_05485"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPL10163.1"
                     /translation="EYTIDIVTQNGDIRNVRAFRKQIVWNGQPHDQIIYQDITRQIQA
                     EEALRESVGNLRKALGGTIRAIASVVETKDPYTAGHQRRVADIARAIAQEMKLPRERI
                     DGLRMAGIIHDIGKVSVPSEILSSPRKLSSLEFSLIKTHAQSGHDIVREIEFPWPIAR
                     IIIEHHERMDGSGYPNALTGDQILPESRILAVADVVEAMATHRPYRPSLGLEAALDEI
                     TQNRGRLYDPEVVDACLRLFREKDYTIRD"
     gene            795..1415
                     /locus_tag="PLQ77_05490"
     CDS             795..1415
                     /locus_tag="PLQ77_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPL10164.1"
                     /translation="MRLFPKEESFFELFETLAEKIEEGGSFFLEMTRGRNYTAARVSR
                     LKEIEHEADVIAHRTYERMHKTFLTPIDREDIYALVNKMDEILDHIEGTAVRVYMYKV
                     KKPNDDIVKQAEILFSAIRIIKKIVHGLRDMKNSRMILDGCVEINTLENAGDVAMRSI
                     ITNLFNTETDAIELLKWKEIFERIEEAIDVCEDVSNILEGIVLKHA"
     gene            1470..2624
                     /locus_tag="PLQ77_05495"
     CDS             1470..2624
                     /locus_tag="PLQ77_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPL10165.1"
                     /translation="MKSEHHGVLYEFILFGFFLASKIMRHKAHLMGKENLSRMPTPVM
                     FAVTHDSYFEVPSLSRIYYALKPRPDFMVMAKNDFLSGRYLSTNYAKKSKFLKSILLM
                     IDRLGLPLAIFRKLKVAAIDRPFVESFNRKKEDLRREISGQLNQFKESSARGISTLIF
                     PEGTTWGYGGLKKIRSSVYHVLENTFQGTGRKVFILPINIKVDRLVKGAKDVFIRIGQ
                     PVFFRKPKDEFNQALFEILQQLHTITFSQVAAYYLKRLAEMKRDAGRTVVFRKELFMR
                     NLEGILLEMSRLVRERALPGFDPGLTNRRYLADKVEHFTKYCLKMQYLQKKRLSDRKE
                     ALLLNVEKILANYSAREFRKANPLGFCANELASLGEQIVRPIFDARLRFR"
     gene            complement(2644..3897)
                     /locus_tag="PLQ77_05500"
     CDS             complement(2644..3897)
                     /locus_tag="PLQ77_05500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPL10166.1"
                     /translation="MSDLIFSLVSWPRAIVHIDGDAFFTSCEEAVHPELRGKPLITGG
                     ERGIVACASYAAKRLGVKRGVPLSDARKMCPDLIILPSDYETYSLFSRRMFAVMRRFT
                     PDVEEYSIDEAFSDITGMRRALKASYEDIALRMKTEIERDLGITVSAGLSITKVLAKT
                     ASKHQKPAGITVIAGRDIARYLQDLPVGSIWGIGPATTSYLAKLGIRTALAFAQMPET
                     TVREKLTKPGVEIWQELRGKSVYPVTSEEKSAYASISKTKTFSPPTSDADYLFAQLAR
                     NLESACLKARHYGLAPRKIVVFLKSRRFETAGSEAALSRPCACPLEVSGVIRKLFNAC
                     RRPHELYRATGVILADLIADECIQYSLFDDPVQAGKIRDVYAAADGLAEKFGKHTVHL
                     GASHLMEKMGDGRRETPAVREQTKT"
     gene            complement(4056..4559)
                     /locus_tag="PLQ77_05505"
     CDS             complement(4056..4559)
                     /locus_tag="PLQ77_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HPL10167.1"
                     /translation="MIEIFINRSSGRLQAAPIRITLKSHRSRHAAAFRQTLFFKIAGL
                     PVSGEVCAGFPSPAEEELRGHISFDEYLVAHPESSFLLSVTGDSMAGAGIMDKDWVII
                     EKNRRPKNGDIILAEVDGAWTIKYYRKKGTRITLETAGAGDPPMVPREELRIAGVVTA
                     LVRKYHR"
     gene            4929..5651
                     /locus_tag="PLQ77_05510"
     CDS             4929..5651
                     /locus_tag="PLQ77_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL10168.1"
                     /translation="MKQWTTKGGQVVRRLSHGMYNCFLVSLNGRHLLVDACRKGSWKK
                     LSRELDELGVNGGSLAGLVLTHTHYDHAENAAAIKDRYNPKIIVHKKEADYVRRGENP
                     VIHGTLPLTGWISDRLSHWYLSQFFRYRPVDGDLTVAERYDLKPLGFQGYLLSTPGHS
                     PGSLSVILENEIAIVGDALFGVFKGSAIPPVAADEKRMVASWEKLLKTGCSLYLPAHG
                     SERGREILERQHEKYKRKYDRP"
     gene            5734..6276
                     /locus_tag="PLQ77_05515"
     CDS             5734..6276
                     /locus_tag="PLQ77_05515"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPL10169.1"
                     /translation="MRKMQLGKAFTLIESGPVVLITTHDGKKNNIMTISWTMVVDFTP
                     VFAITTGPWNYSFAALQKTKECVMAVPTVDLLDRVVGAGTCSGADTDKFEKFLLTPVK
                     GKHVQAPLIKECLANIECKVVDIVRKHNIVVLEAVAAYFDPSRKERRTLHAVGDGTFI
                     VDGRKLDRREMMRSKIPAGL"
     gene            6458..7999
                     /locus_tag="PLQ77_05520"
     CDS             6458..7999
                     /locus_tag="PLQ77_05520"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPL10170.1"
                     /translation="MIKTVEQYLESLNDGREVWCLGEKVKDVRTHPTVSTIVRMAAMD
                     YVLPNHPDFRDLFVTKDADGEDINFLLTSPKTPQDMLRRRECYMAGMRTGGGVLVHCM
                     GADALASFSVAAEVMDKKMGTGYTERVENYRRHLQKNDLALTGAITDVKGDRSLHPSQ
                     QKQHKDFYLRIVDRQKDGIVVRGAKVHISASPCANELLCLPCRTHGEADKDYALGFAI
                     PVAAKGVKLLGVEPNVRLYGEECEFDYPTSDHLQPSESLIVFDDVFVPWERVFMCGEW
                     QYSQVLAYAFASYHRLFGTCKMTGTLELMTGAASLIAEYNGVQNMPHVRKKLAWMAYI
                     TETVQMLAKQACLDPVTEFGMDVMMPNRMAINASKWTYASNFHTMCQHMQDIGGGLTT
                     TVPAFRDWDNPEIKPFIDKYLAAKDGVSTEERLRILRLIKDGTGNHWQVDTIHGEGSM
                     AAQEMFIYGSANWKKFQAAAKRAAHMDGWQDDTTYGKLPLMDQCVQMPPIDETYKSFA
                     PTAKK"
     gene            complement(8209..8285)
                     /locus_tag="PLQ77_05525"
     tRNA            complement(8209..8285)
                     /locus_tag="PLQ77_05525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8249..8251),aa:Arg,seq:ccg)
     gene            complement(8364..9923)
                     /locus_tag="PLQ77_05530"
     CDS             complement(8364..9923)
                     /locus_tag="PLQ77_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPL10171.1"
                     /translation="MPENRFKEALLNPSKFPVTWELVPGRGAKEAAQEKCLAQARQAA
                     GGDKVCALTLTDNPGGTPAMSADFMGAEILRLGIEPLVHFTGKDKNRNQSESQLYALD
                     RAGVRNLLVMSGDYPVSGFQGRPAPVFDLDPIHTLQLISNMNRGLAYPGIKGAVTHQP
                     SDFFPGAVVSPFKATESEQVGQYDKLKKKIDAGAKFIVTQLGYDARKFHEALLFIKQN
                     YPQIPLVGNIYILPFGAAKMMNRNDLPGSVVTDQLLADIDRERSDPDKGEGARILRAA
                     RMYAILKGMGYSGVHIGGHNIRYEQVEEIIRQGEALVPQWTNLVRYFHYPIDNGFYFF
                     EADPATGLNRDTPTDRQCRPPDAKVGWMYGFSRFFHRLMYEPGKNLYGAMKALCRTVQ
                     GTRLEGIFHKLEHLTKVVLFDCRDCGDCALPDAAYLCPMSQCPKNQRNGPCGGSFQGW
                     CEVYPQKRLCIYVRAYARLKKHGEESRLAGNIVPPCNWDLYQTSSWINYYLGKDHTSA
                     KQGGAGSPGAP"
     gene            10259..11902
                     /locus_tag="PLQ77_05535"
     CDS             10259..11902
                     /locus_tag="PLQ77_05535"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPL10172.1"
                     /translation="MRHWAAPDQTRIVFDLSDEPAYRFEIQQDRIILEFSDASFSPSL
                     PSEKIVRKPGVAKILFIRGEDQLCTVQIHLKDHLRAEVFPLKKFMDKPDRVVVDVFLA
                     EAEKAPAVVKRKAVSAPRRVIVIDPGHGGEDPGAIGKNGTYEKHVVLAISREIKKTID
                     KMPGYRAVLTRDGDYYVSFSKRLGVARKHQASLFISVHADAARNREAQGSSVYCLSTG
                     AASNEAAKLLARNENLSDIIGGVPGGEGNNQSDEIILNMFQTNTINLSKTYAADLLDQ
                     IGRVQMLKYPTFHEAPFRVLKLPDTPAVLLETAYLSNPQEEKLFKTQSFRKNIARAVA
                     SSVDRYFAGSNAVANAVAESGGEDAGESRDAKQPKKAAGAAGAEGRMKTAAYTVVRGD
                     TMQKIAQKHETDLAALLTLNNMKIHDPLFAGRKIRVPAAGAAGAEGRMKTAAYTVVRG
                     DTLQKIAQKHETDLAALLTLNNMKIHDPLFAGRKIRVPAAGAQTDGKRRLKKYTVQKG
                     DTLFSLAKSRSTTMEELRRINQMTDADVLRVGQSIRLPE"
     gene            11932..>12253
                     /locus_tag="PLQ77_05540"
     CDS             11932..>12253
                     /locus_tag="PLQ77_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10173.1"
                     /translation="MTAATQQALSGLRDLSTIQWYVIPLLAIVIYIYAREIREARKTA
                     DWNAVLAGLTIFGVDFFNETWNGWVLHFTGRSAFWTTPGETALRTMVGWNIEIMFMFL
                     LVGII"
BASE COUNT         2700 a         3400 c         3546 g         2607 t
ORIGIN      
        1 cgaatatacg atcgatattg tcacccagaa cggcgacatt cgaaatgtcc gggctttccg
       61 caaacagatt gtctggaacg ggcaacccca tgatcagatt atctatcagg acattacccg
      121 tcaaatccag gccgaagaag cgctgcggga aagtgtcgga aatctgagga aagccctggg
      181 cgggaccatt cgggccattg cctccgtcgt ggaaaccaaa gatccctata cggcgggcca
      241 tcagcgccgg gttgccgata tcgcacgggc catcgcccag gagatgaagc tgccgcggga
      301 gcggatcgac ggcctgcgga tggccggcat cattcacgac atcgggaaag tctccgtgcc
      361 ttccgagatc ctcagttcgc cgaggaaact gtcgtctttg gaattcagcc tgatcaagac
      421 gcacgcgcaa tccggccatg acatcgtccg ggagattgaa tttccctggc ccatcgcgcg
      481 gataatcatc gagcatcatg aacggatgga cggctccggg tatcccaacg ccctgaccgg
      541 ggatcaaatt ctaccggagt cgagaatcct ggccgtggcc gacgttgtgg aagcgatggc
      601 gacgcatcgt ccctaccgcc cgtccctggg gcttgaggcc gccctggacg agataaccca
      661 aaaccggggc cggctttacg atccggaggt tgtggatgcc tgcctgcgcc ttttccggga
      721 aaaggactac accatccggg attgacgcta tcgtttcagg cgctgcccaa aaaatcaaag
      781 gagatatttt tcacatgcgt cttttcccga aagaagaaag tttttttgag ctctttgaaa
      841 cgctggccga aaagattgaa gagggcggat cttttttcct ggagatgacc cgcgggcgga
      901 attacaccgc ggcccgggtt tcccggctga aggaaatcga gcacgaagcg gacgtcatcg
      961 cccatagaac ctacgaacgg atgcacaaaa cctttctgac gcccatcgat cgcgaagata
     1021 tttacgcgct ggtcaataaa atggacgaaa ttctggatca catcgaaggc accgccgtcc
     1081 gcgtgtatat gtacaaggtg aaaaagccga acgacgacat cgtgaaacag gcggaaatcc
     1141 ttttttcggc gattcggatc atcaagaaga ttgttcacgg gctgcgcgac atgaagaaca
     1201 gccggatgat tctcgacggc tgcgtcgaaa tcaacaccct ggaaaatgcc ggcgatgttg
     1261 cgatgcggtc catcatcacc aacctcttca acaccgagac ggacgccatt gaactcttga
     1321 aatggaaaga aatctttgaa cgaatcgaag aggcgatcga cgtctgcgaa gacgtttcca
     1381 acattctgga aggcattgtc ctgaagcatg cctgatcggc ggagcccttc accgtttttc
     1441 agagctctcg gggcgaaagg agcgcgaaaa tgaagtcgga gcatcacggc gtattgtatg
     1501 aattcatttt gttcgggttt tttctggctt ccaaaatcat gcgccacaag gcccatctga
     1561 tggggaaaga gaatctttcc cgaatgccca ctccggtcat gtttgccgtg acccacgaca
     1621 gctatttcga agttccgtcc ctgtcgcgca tttactatgc actgaaaccc cggccggatt
     1681 tcatggtcat ggccaaaaac gactttttga gcggccggta tctttccaca aactacgcga
     1741 aaaaaagcaa gtttctgaaa agcattctgc tcatgatcga ccgactgggc cttcctctgg
     1801 cgattttccg caagctgaaa gtggcggcca tcgaccgtcc gttcgtggag tctttcaacc
     1861 ggaagaaaga ggacctgcgg agggaaatct ccggccagtt gaatcaattc aaggaaagca
     1921 gcgcgcgggg gatatcgacg ctgatttttc ccgagggaac gacctgggga tacggcggcc
     1981 tgaaaaaaat tcgcagctcc gtgtatcacg tcctggaaaa tactttccag ggcaccgggc
     2041 ggaaagtgtt tattctgccc atcaacatca aggtggaccg tctcgtgaag ggggcgaaag
     2101 acgttttcat ccgcatcggt caaccggtgt tttttcgcaa gcccaaggat gaattcaatc
     2161 aggcgctctt tgagatcctc cagcagcttc acaccatcac cttcagtcag gtcgccgcct
     2221 actacctcaa gcgcctcgcg gagatgaagc gggacgccgg ccggacggtc gtttttcgca
     2281 aagaactctt tatgcggaat ctggaaggga ttctcctgga gatgagccgg ctggtccgcg
     2341 aacgggcgct gcccggtttt gaccccggcc tcaccaatcg gcggtatctt gccgacaagg
     2401 tcgaacattt tacaaaatac tgcctgaaga tgcagtattt gcagaaaaag cgtttgagcg
     2461 accggaaaga ggcgctgctt ctcaacgttg aaaaaattct ggcgaactat tccgcccggg
     2521 aatttcgtaa agccaatcct ctgggattct gcgccaatga actggcgtca ctgggcgaac
     2581 aaatcgtccg ccccatcttt gacgcccgcc tccgttttcg ttagcggcgg ttttttgctt
     2641 ctttcacgtc ttcgtctgct cccggacagc gggcgtctct cttctcccgt cgcccatctt
     2701 ttccatcagg tgtgatgcgc ccaggtgcac agtgtgcttg ccgaatttct ccgccagtcc
     2761 gtcagccgcg gcgtaaacgt cgcggatttt tcccgcctgc accggatcgt cgaagagcga
     2821 atactgaatg cattcatcgg caatgaggtc cgcaagaatc acgcccgtcg cccggtacag
     2881 ttcgtgcggc cgccggcagg cgttaaaaag tttccggatg acgccggaaa cctccaacgg
     2941 gcaggcgcag gggcgcgaca gagccgcttc gctcccggcc gtttcgaaac gccggctttt
     3001 cagaaacacc acaattttcc tcggcgcgag accgtagtgc cgggccttga ggcacgccga
     3061 ctccagattg cgcgcaagct gcgcgaaaag atagtcggcg tcggaggtcg gaggagaaaa
     3121 ggttttggtt ttgctgatgg aggcataggc gcttttttct tcggacgtca ccggatacac
     3181 cgacttgccg cgcagttcct gccagatttc cacacccggt ttggtgagct tctcccgtac
     3241 cgttgtttcc ggcatttgcg cgaaggccag cgccgtgcgg atgccgagct tggccagata
     3301 gctcgtcgtg gccgggccga ttccccagat gcttcccacc ggcaggtctt gcagatagcg
     3361 ggcgatgtcc cggccggcaa tcacggttat gccggcgggc ttctgatgct tggaagccgt
     3421 tttggccaga accttggtga tgctcagccc ggccgacacg gtgatgccca ggtcccgttc
     3481 gatttccgtt ttcatccgca gggcgatgtc ttcgtaggaa gccttcagcg cccggcgcat
     3541 gccggtgatg tcggaaaacg cctcgtcgat ggaatattcc tccacgtcgg gcgtgaagcg
     3601 gcgcatgacc gcaaacatcc gccgggaaaa gaggctgtag gtttcgtaat ccgacggcag
     3661 aatgatcaag tccggacaca ttttgcgcgc atcggaaagc ggcacgccgc gtttgacccc
     3721 cagccgcttg gccgcgtagc tggcgcaggc gacgattccg cgctcgccgc cggtaatgag
     3781 cggcttgccc cgaagctccg ggtgcaccgc ctcttcacag gacgtaaaaa aggcgtcgcc
     3841 gtcaatgtgg acaatggcgc gcggccagga aacaagagaa aaaattaaat cggacataaa
     3901 aaatcagacc ataggttagg ggtcaggggt cagggaccat aggtcatagg taatgggtaa
     3961 ttgggtagtg ggtaaaaaga gtgtcatggt gagccagtcg aaccatgacg gggcatccac
     4021 catccaccat tcaccatcca ccatcgcttc ttgccttatc gatgatactt cctcaccagc
     4081 gccgttacca ctcccgcgat gcgcagctcc tcacgcggga ccatcggagg atctccggcg
     4141 ccggccgttt ccagtgtgat gcgggttccc tttttgcggt agtattttat cgtccaggcg
     4201 ccgtccactt ccgccagaat gatatccccg tttttcggcc gccggttttt ttcaatgatg
     4261 acccagtcct tgtccatgat cccggcaccg gccatggaat cgcccgtgac ggagagcaga
     4321 aacgaggatt ccggatgcgc caccagatac tcgtcaaaag aaatatgacc gcgcagctcc
     4381 tcttccgcgg gagaagggaa gccggcgcaa acttccccgc tgaccggcaa ccccgcgatt
     4441 ttgaaaaaaa gagtctggcg gaaagcggca gcatgcctgc tccggtgtga ttttaaggtg
     4501 atccgtatgg gcgcggcctg aagccggccg ctggaccggt tgataaaaat ctcaatcatc
     4561 ttcaatgctt tcctgttttt ttcttcccgg ggtgtaagag ggtgaagccg cttccacggc
     4621 cgggatttct tcggatttct ccggaaactc tctatataga acgtacgttc tattgtcaag
     4681 gcaaatccac ccgccggtgc cgggtgcgac cctgtcctca aagcgatgaa aatgcctgaa
     4741 aaccgcttcg gtttgtggta gatgcatcag gtaaggcgtc cttccccgct gccggcaagt
     4801 tgacgccggg cgtgcgggtg gttccggcgg acgtgatgcg tcggggagag gccggggcgt
     4861 cacgattcgg gctgtagttg tagacggacg tccgcaacgc aaacgatttt cggccgaagg
     4921 aggaagggat gaagcaatgg acgacaaaag gcgggcaggt tgtccgcagg ctttcccacg
     4981 gcatgtacaa ctgctttctc gtttccttga acggccggca tctgctggtg gatgcctgca
     5041 gaaagggaag ctggaaaaag ctctcccggg aattggatga actgggcgta aacggcggct
     5101 cgctggccgg cctggttctg acgcacaccc attatgacca cgcggagaac gcggcggcga
     5161 tcaaagatcg ctacaaccca aaaatcattg tccacaagaa agaagcggac tacgtgcgca
     5221 ggggcgaaaa tcccgtgatc cacggaacgc tgccgctgac cggatggatc tctgaccgcc
     5281 tgtcgcactg gtatctttca cagttcttcc gctaccggcc cgtcgacggc gacctgacgg
     5341 tcgcggaacg gtacgattta aagcctctgg ggtttcaagg gtatcttctt tccacgccgg
     5401 gtcattcgcc cgggtccctc agcgtcatcc ttgaaaacga aatcgcgatc gtcggcgatg
     5461 ccctgttcgg cgtctttaag ggatcggcga ttcctcccgt cgcggcggat gagaagcgca
     5521 tggtggcaag ctgggaaaag ctgctgaaga cgggctgctc tctttatctt ccggcgcacg
     5581 ggtcggagcg cggcagagag attctcgagc ggcagcatga aaagtacaaa cggaaatatg
     5641 accggccgta gcgccgggtc ttcggcaatc cgggtttgaa agacggacgg cgtcggcatg
     5701 gtcgggccaa cagggaaagg agcgtgtgcg cacatgagaa aaatgcagct cggtaaagcg
     5761 tttacgttga tcgaatccgg tccggttgtc ctcattacga cacatgacgg aaagaagaac
     5821 aacatcatga cgatctcgtg gaccatggtg gtggacttta cgccggtatt tgccatcacc
     5881 accgggccct ggaactattc cttcgccgct cttcaaaaaa cgaaggagtg cgttatggcc
     5941 gttcccacgg ttgatctgct ggatcgggtc gtcggcgcgg gaacctgttc cggcgccgac
     6001 acggacaaat ttgaaaaatt cctgctgacc cccgtgaagg gaaagcatgt ccaggcgccc
     6061 ctgataaaag aatgtctggc gaatatcgaa tgcaaagtcg tggacattgt ccggaagcac
     6121 aatatcgtcg ttctcgaagc cgttgcggcg tactttgatc cttcacgaaa ggagaggcga
     6181 acgcttcacg ccgtcggcga tggaactttt atcgtggatg ggcgcaagtt ggaccgcagg
     6241 gaaatgatgc gctcaaaaat tccggccggt ctctgatgac agcacatcca gggggtccgc
     6301 cggtttccga acctgtatgg aggatgcctg ctgctggtcc acccggttgc attactattt
     6361 tgacatgctt cattgaaagg agcatggagg aacaggcatt ttgctcatta aaaatatttt
     6421 ttaagctttc tctcacaacg aataagggaa aataaaaatg atcaagaccg ttgagcagta
     6481 cctggagagc ttgaatgacg gccgggaagt ctggtgcctg ggagaaaaag tcaaggacgt
     6541 gcggacgcat ccgaccgtga gcacgattgt ccgcatggcg gcgatggact atgttctgcc
     6601 gaatcatccc gatttccggg atctgtttgt tacgaaagac gcggacggag aggatattaa
     6661 ttttcttctc acctcgccga aaacgccgca ggacatgctg cgccggcggg agtgctacat
     6721 ggccggcatg cgaacgggcg gcggtgtgct ggtgcactgt atgggcgccg atgccctggc
     6781 ctcgttcagt gttgctgcgg aagtcatgga caagaaaatg gggacgggct acacggagcg
     6841 cgtcgagaat taccgccggc atctccagaa aaacgacctg gcgctgaccg gcgcgatcac
     6901 cgacgtgaaa ggcgaccgca gccttcaccc ctcgcagcag aagcagcaca aggattttta
     6961 cctgcggatc gtcgaccgtc agaaagacgg cattgtggtg cgcggcgcca aggtgcacat
     7021 cagcgcctcg ccctgcgcca acgagctttt gtgcctgccg tgccggacgc acggggaagc
     7081 ggacaaagac tatgcgctgg gctttgcgat tccggtcgcc gcaaagggcg tcaaactcct
     7141 gggggttgag cccaatgtcc ggttgtacgg ggaagaatgc gaattcgatt acccgacctc
     7201 ggatcatctg cagccttcgg agtccctcat tgtgtttgac gatgtgtttg ttccctggga
     7261 gcgggttttc atgtgcggcg agtggcagta ttcgcaggtg ctggcgtatg cgtttgccag
     7321 ctatcatcgt ctgttcggca cctgcaagat gacgggaacg ctggaactga tgacgggcgc
     7381 ggccagtctc atcgctgaat acaacggcgt ccagaatatg ccgcatgtcc gcaaaaaact
     7441 ggcctggatg gcctacatca cggaaaccgt ccagatgctc gccaagcagg cctgtctcga
     7501 tcccgtaaca gagttcggca tggacgtcat gatgcccaac cgcatggcga tcaacgcttc
     7561 aaagtggacc tacgcgagca acttccatac gatgtgccag cacatgcaag atatcggcgg
     7621 cggacttacc accaccgttc ccgcttttcg ggattgggac aacccggaaa tcaaaccgtt
     7681 tatcgacaag tatctggcgg cgaaagacgg cgtttccacc gaagagaggc tgcgcatatt
     7741 gcggctgatc aaggacggta cgggaaatca ctggcaggtc gatacgattc acggcgaagg
     7801 atcgatggcg gctcaggaga tgtttatcta cggcagcgcc aactggaaga aattccaggc
     7861 ggcggcaaag cgggcagcgc acatggacgg ctggcaggac gataccacgt acggcaagct
     7921 gcccctgatg gatcagtgcg ttcaaatgcc cccgatcgac gaaacctata aaagcttcgc
     7981 gccgacggcg aaaaagtgaa gaatacggtt tttcaatcca tcaaaagcgg ctgttttccc
     8041 gaacaagatg ctatccgttt tgatccattg aaccgtggga cattcaacgc gcatccccgg
     8101 gaagcggatg aagctctctt tcttgattgg cctgtgtgaa gaagggcaaa aaacccgcta
     8161 ctcctggcgg gtttcgtgca ctttcttgga ttgtatcgga tgatggcttg gtgggcaatg
     8221 agagattcga actctcggcc tatggcttcg gaggccagcg ctctatccag ctgagctaat
     8281 tgcccacgcg ggtgcttcta cactatcccc tcggaatctt caacggttaa attccttttc
     8341 ctgaaaacga ggggagaaga ctgtcaggga gcgccgggcg atcccgcacc gccttgtttt
     8401 gcggatgtat ggtctttccc cagatagtaa ttgatccagg aggaggtttg ataaaggtcc
     8461 cagttgcagg gcggcacgat gttcccggcc agacgcgatt cctccccgtg ttttttcaac
     8521 cgggcgtaag cccggacgta gatgcacagc ctcttttggg gatagacctc gcaccatccc
     8581 tgaaaacttc cgccgcaggg gccgttgcgc tgatttttcg ggcattgcga catggggcac
     8641 agataggccg cgtcgggaag ggcgcagtcg ccgcagtcgc ggcagtcaaa caggacgacc
     8701 ttggttaaat gttccagctt gtgaaaaatc ccctccagcc tggttccctg cacggtccgg
     8761 cacagcgcct tcatcgcgcc gtagaggttt tttccgggtt catacatcag ccggtgaaaa
     8821 aagcgggaaa atccgtacat ccacccgact ttcgcatccg gcgggcggca ctgccgatcg
     8881 gtgggggtgt cccggttcag tccggttgcc ggatcggctt caaagaagta aaacccgttg
     8941 tcaatgggat aatgaaaata ccggaccaga ttggtccact gcggaacaag cgcttctccc
     9001 tgccgaatga tctcttccac ctgttcgtat cgaatgttgt ggccgccgat gtgaacgccg
     9061 ctgtagccca ttcccttcag aatggcgtac attcttgccg cgcgaaggat tcttgcgcct
     9121 tcccccttgt ccgggtcgct tcgctctcta tcgatgtccg caagcagttg atccgtgacg
     9181 acggatcccg gcaggtcatt gcggttcatc attttcgccg cgccgaaggg aaggatgtaa
     9241 atgtttccca ccagggggat ctgcggataa ttctgcttta tgaagaggag cgcttcatgg
     9301 aatttccgtg cgtcgtatcc cagttgcgtg acgatgaact ttgcgccggc gtcgattttc
     9361 tttttcagct tgtcgtattg ccccacctgc tcggattctg tcgccttgaa gggggagacg
     9421 accgcgcccg gaaaaaaatc ggaaggctga tgggtgacgg ctcctttgat gccgggatag
     9481 gcaagccccc ggttcatatt cgagatcaat tgcagcgtat gaatgggatc caggtcgaaa
     9541 accggcgcgg gccttccctg aaaaccggac acgggataat cgccgctcat gacgaggaga
     9601 ttccggacgc ctgcgcgatc cagcgcgtaa agctggcttt cagactggtt gcggtttttg
     9661 tccttgccgg taaaatggac gaggggttcg atgcccagcc ggaggatttc ggcgcccatg
     9721 aagtcggcgg acatggccgg ggttccgccc ggattgtccg tgagcgtgag cgcgcagacc
     9781 ttgtctcccc cggctgcctg ccttgcctgg gccaggcact tttcctgcgc ggcttctttc
     9841 gctcctctgc ccggcaccag ttcccaggtc acggggaatt tggagggatt cagcagggcc
     9901 tctttgaacc ggttttccgg catgcttttc ctcaagaatg gattcgctgt ttttgcgcgc
     9961 tgaaaatcgc atttacgaaa aagcttgtca actgttttta gcgcttgaag acaacctttc
    10021 cttgcagttg cccctgaaat ttgttactca aatcggcatg aatccggcaa cgagcgcatt
    10081 ttccgccgcc cgcggcggga tggacgggcg gcactccatt tccatcggct ccccgcgaac
    10141 cgcgcgggag agttccgtcc ttgtgtgtct tagggcctgg cggctctggt gttgcctggc
    10201 ctttttcctg ccggcatttt tccccgctcc ggggtattcg ctaacggaga tatccaatgt
    10261 gaggcattgg gcggcgcccg atcagacgcg cattgttttc gatctcagcg acgaacccgc
    10321 ataccggttt gagattcagc aggacaggat catcctggaa ttctccgacg cctccttttc
    10381 tccctctctg ccgtctgaaa aaatcgtccg aaagccgggc gtcgccaaaa ttctttttat
    10441 acgcggggag gatcagctct gcacggttca gattcatctg aaagatcatt tgcgcgcgga
    10501 agttttcccg ctgaagaagt tcatggacaa gccggaccgc gtcgtggtgg atgtttttct
    10561 ggcggaggct gaaaaagctc ctgccgttgt gaaacgcaag gccgtctcgg cccccaggcg
    10621 ggtcatcgtg atcgatcccg gccacggcgg agaagatccc ggcgccatcg gcaaaaacgg
    10681 gacgtacgaa aagcatgttg tgctggccat cagccgggag atcaagaaaa ccatcgacaa
    10741 gatgcccggg tatcgggcgg tcctgacccg cgacggagat tattacgtat ccttcagcaa
    10801 gcgcctcggc gtggcgcgca agcaccaggc aagcctcttt atcagtgtcc acgcggatgc
    10861 agcccgcaac cgtgaggccc agggcagctc cgtttattgt ctgtccacgg gagcggccag
    10921 caacgaagcc gccaaattgc tggcccggaa tgaaaacctc tccgacatca tcggcggcgt
    10981 gcccgggggc gaaggcaaca atcaatccga cgaaatcatc ctgaacatgt ttcaaaccaa
    11041 caccatcaac ctgtccaaga cctacgcggc cgatctgctg gaccaaatcg gccgcgtaca
    11101 gatgctgaaa tatcccacgt ttcacgaggc gcccttccgt gtgctgaagc tgccggatac
    11161 ccccgccgtg cttctggaaa ccgcctacct ttccaatccg caggaagaga agctgttcaa
    11221 aacgcaatcc ttcaggaaaa acatcgccag ggcggtggct tcgtccgttg accgctattt
    11281 tgccggatcg aatgcggttg cgaatgcggt tgccgagagt ggcggcgaag acgcggggga
    11341 aagccgggat gcaaagcagc ccaagaaggc cgccggggcg gcgggcgccg aaggcaggat
    11401 gaaaacggcc gcgtacacgg tcgtgcgggg cgacacgatg cagaagattg cgcagaagca
    11461 cgagaccgac ctcgccgccc ttttgacact gaacaatatg aagatccacg atcctctttt
    11521 tgccgggcgg aaaatccgcg ttccggccgc cggggcggcg ggcgccgaag gcaggatgaa
    11581 aacggccgcg tacacggttg tgcgggggga cacgctgcag aagattgcgc aaaagcacga
    11641 gaccgacctt gccgcccttt tgaccctgaa caacatgaag atccacgatc ctctttttgc
    11701 cgggcggaaa atccgcgttc cggccgccgg ggcgcaaacg gacggcaaaa gacgcctgaa
    11761 aaaatacaca gtccaaaaag gcgacacgct gttttcgctg gcgaagagcc gttccacgac
    11821 catggaagaa ttgcggcgca tcaatcagat gacggacgcc gatgtgttgc gggtggggca
    11881 gagcattcgg ctgccggagt aagacaacca gatatttgaa aggaggcttt catgacggca
    11941 gcaacccaac aggctctaag cggtcttcgc gacctttcca ctattcaatg gtatgtgatt
    12001 ccgcttctgg cgattgtgat ttacatttac gcccgggaaa tcagggaagc caggaaaacg
    12061 gcggactgga acgccgttct ggccggtttg accatctttg gcgtcgattt cttcaacgag
    12121 acctggaacg gctgggtcct tcatttcacg ggccggtccg ctttctggac cacgcccggc
    12181 gaaacggcgc tgcgcacaat ggtcggctgg aacattgaaa tcatgttcat gtttctgctg
    12241 gtgggcatca tct
//
LOCUS       DAOPAF010000044        12197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5153_length_12197_cov_46.173942, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000044 DAOPAF010000000
VERSION     DAOPAF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5153_length_12197_cov_46.173942"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1722
                     /locus_tag="PLQ77_05545"
     CDS             124..1722
                     /locus_tag="PLQ77_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPL10174.1"
                     /translation="MLKFFRKHARGWFMLVFMGIIIFVFVLYFGTDRSMQTANALAVV
                     DGYVVTESEYYNEYSKLTDMIRAQYGAALTADMLKQMDLKKIAYDNLINRQIIIARAK
                     DMKMQVSEDELRQMIMAMPALQTDGRFDNYKYQQLLRFNRMTAEDFEAGQKITLAAGK
                     IEAIIREGIKVSDREVLDLYTLQNQKINLGFIAVAGADVAGKINPATPDLEEHLKKNS
                     GAFRVAEQVKIQYLHFAGEAFAPAQITASDIRDHYNRHKDSYKGKDGKPLSPEQAAPI
                     IAREMKQSRGLQDAYLEAKKAHDTIYQEQNFEDYAAKHQLKTQTVDFFPLNKPPQALA
                     SVKDLAQELAALERKDISKVMSDANGYFVIRLDDKKAAYTPPLKAIEPEVRQAYLKSE
                     QDRMAAQEAESLLAKLRKGESMEKLAGEKGLRIQETGFFQPGGVIPKLGANPDAVNVL
                     MSLSPSRPYPEKPMVIDHSHVIFRLKDVSALDMNDFEAKKEIYRKVAMNLKREEAMKT
                     WLEGNKEALMKEKRLKIQKELKDL"
     gene            complement(1849..1924)
                     /locus_tag="PLQ77_05550"
     tRNA            complement(1849..1924)
                     /locus_tag="PLQ77_05550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1889..1891),aa:Ala,seq:ggc)
     gene            2029..2115
                     /locus_tag="PLQ77_05555"
     tRNA            2029..2115
                     /locus_tag="PLQ77_05555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2063..2065,aa:Leu,seq:caa)
     gene            2537..3664
                     /locus_tag="PLQ77_05560"
     CDS             2537..3664
                     /locus_tag="PLQ77_05560"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPL10175.1"
                     /translation="MKNVIMFLCAVLALGCFPVSVQSAIAAEPSVYVDYEYGDLKEVI
                     VGLPYGMSPSLEAKWFADALKVLPPDEADYARKTAGMLWTEMINPETGKSETRMLEEE
                     NQAFIGILKSLGVKVYRPTEITVDFIKEHYGADVLLNGFSQDFPRDNMAVIGTNVIEF
                     NLRTPIRKADISGFRNILSEKCSDPAVKWFSMPHTELLKPARPDTPFLEGGDVIVLGK
                     TVLVGNTQNPSVGSNEAGCRWLKNILGDQYQVKRVRLVESVLHLDCVLSVPREGLAII
                     CEEAFVDGLPKELKGWDLIRVSLKDVQRLAVNGVPVNSENYILSYNRHNDNRAIQAEL
                     EKRGIRVHRVFFGTHNGQGGSLRCATQPLKRKVRMKKANGG"
     gene            complement(3806..5020)
                     /locus_tag="PLQ77_05565"
     CDS             complement(3806..5020)
                     /locus_tag="PLQ77_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPL10176.1"
                     /translation="MQALQFNVNVPKFLIIQALRPISASFCYSGPFATVKLVEMPEPK
                     LPAPEWVKIKTRLCGVCGSDMNLIFLKDSPTASPFTSFPCVPGHEICGEIVETGADVS
                     HLKNGDRVVVVPALNCYARAIRPVCRSCAAGTTANCENFAEGAFSPGMFIGICKDING
                     GFAEFFVAHKSQVHLVPDGVSTESAALVEPLSVGLQAVLDNRPRDTDKVLIIGGGVIG
                     AMVVKSMRALDIGCDITVVEPSSFASEHLKACGAHRTIRGGIIDAAVEITGAHAYQPL
                     LGERVLMGGFDKIYDTVGHADTLNLALRVLTTNGTLSVIGIGKEVKLDLTTLWLKLQT
                     VKGCYGYRYNHTANGGKHTYQMALELISSRKIRVDDLLTHKFPLERYREMIEVNLNKE
                     ANRAMKTAVSFE"
     gene            complement(5055..6725)
                     /locus_tag="PLQ77_05570"
     CDS             complement(5055..6725)
                     /locus_tag="PLQ77_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPL10177.1"
                     /translation="MGMINGGHLVGKYLKEVEKVEIVFGISGGHLESILDSFTEYRIR
                     TIDVRHEQAAAMMAHAWSVYTGKPGVCFLTTGPGFTNGLTGIANACLDNAPMVVICGK
                     HPLRDDLRGALQEMNQLDVVKPITKWAATCYDIKRIPEYLAIAFRQAVEGRPGPVMLE
                     LPPDILNIKVDESLAPMPSRSTRKYTVHPDDADLSAAADLINNAAKPLFIGGTGVGFS
                     SCEDCLGQFIDKTGMPFILLNNGRGTLPDNHPLSVWDCGNVGLMVAIAQADVIVIAGI
                     RLNWLLQSGAVIPPATKVVRIDIDPHEIDRNRQADAGLVGDIGSVLGQLNPLLVKKDR
                     GEWVTLLRAASRSMIDGELKIRQTASDPIHPIRLAEQIQSCVGDDAFYVIDGGDTSYF
                     GLVGFMSRHQAGVISQSSGLLGCLGTGIPFAMAAKLAHPDKKVLLFNGDGSFGFNAME
                     FDTMVRHNIPVVCVVNNDCAWGMIKHTQEMSIGCDRLQCSELGTRHYEKMVEGLGGHG
                     EFAAKDEDIIPALNRAFQSGKPACVNVMTDPTVTSPASVLFYQSLKME"
     gene            complement(6743..7102)
                     /locus_tag="PLQ77_05575"
     CDS             complement(6743..7102)
                     /locus_tag="PLQ77_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10178.1"
                     /translation="MKLRLLLFILYQKLKKAAAKNAAYRSFLGGMQVKIMIQTADGKH
                     GRLFVFDRGKLSTLSGCGHSYDAALVWSDSGTAFQVMSSGSDEQTFLAAAGGKLKIDG
                     MAAYVQWFNDGVKLVMN"
     gene            complement(7105..8091)
                     /locus_tag="PLQ77_05580"
     CDS             complement(7105..8091)
                     /locus_tag="PLQ77_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPL10179.1"
                     /translation="MNALVTGATGFIGSVLVRDLLARGSSVRALVLPGENADVLKKQG
                     VRVIRGDLTDPESLRGVCEGIETVFHLAGRVTDWGTKRQFYTAICDATGNLLQEAAGR
                     ASRFVYVSSIAAMGMGRHLKGVKESDPPRKSGVSYNDAKVDAEALVRAFHREDKMKCT
                     IVRPANVVGPGSVWVRDIADRMMSATGVPLIDGGRYSASFVYVDHLVDGMILAAAGDI
                     ACGKAYHFRDDWRVNWKEYITDLGSWIGKRPRGSIPFRLAWMTGFVLEAVLNPLHIRA
                     PLTRLSAGVLGRDNDVDTMLARTELGWRTRLSYPEAMEKIGAWVKDHYQIGR"
     gene            complement(8613..9503)
                     /locus_tag="PLQ77_05585"
     CDS             complement(8613..9503)
                     /locus_tag="PLQ77_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10180.1"
                     /translation="MRTYTLKYVELAEQHAEKMAKRWALDVQNNAKTPTYKNLNEQKI
                     IFQCVQFYRNFSKMFVHEKITEEVQKYFRIYATECYALGIPMAEMVYALILMRRHIWL
                     YAEFQMIFSSLINQQQALDTLNRTILLFDYASYDVTREYQELAKRDKLESIRLLDILE
                     SNVVEIASNWAATVRKDKQTVFYHGIPKDKLIPQAIKFYSRLRTLLFDPERFEKGREF
                     FRQYAETCRQQGIPLHEAIYALNVMRRQMWLHGEFQRTFVNALAHQQAVDSLMRIILL
                     MDYAAYDITHYYQERMLLED"
     gene            complement(9544..10170)
                     /locus_tag="PLQ77_05590"
     CDS             complement(9544..10170)
                     /locus_tag="PLQ77_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPL10181.1"
                     /translation="MPKIELPPTKSSLRRLKEDLAFAYEGYDLLNQKREILAIEIVRN
                     IGEIRRIEAEFQQGLNRLYGAYRGAAVDMGSDVASLQSCKEVRSYFLRTDYAKLMGLR
                     MPVIRLTLKNPHPAFTLPQTTAAYDEARDQSRKTLLTLAEYATMTKSVMMLSRELKKV
                     QRRVNALEKIFIPQHEESKKYITDRIEEMEREEVFVKKLIRQRGQSRI"
     gene            complement(10431..11822)
                     /locus_tag="PLQ77_05595"
     CDS             complement(10431..11822)
                     /locus_tag="PLQ77_05595"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPL10182.1"
                     /translation="MSAEIEYTNLSGIAGPLIFVDGIQGVGYGDIVEIRDASGELRTG
                     QTLEVDEQRAVVQVYEGTSGLSVKGTRVTFVGRALEIPVDRDMLGRVYDGLGRPIDGA
                     PQVITDRMMNVNGLPINPWSREYPRDFIQTGISAIDGMNTLIRGQKLPIFSGSGMPHN
                     RIAAQIARQARILTAEEEFTVIFAAMGVKFDVARFFIDSLEESGVLTHSAIFLSLADS
                     PSIERLVTPRTALTLAEYLAFEEGMHVLVILTDMTNYCEALRELSSSRGEIPSRKGYP
                     GYLYSDLASVYERAGRLKGGKGSITQVPILTMPSDDISHPVPDLTGYITEGQIVLERA
                     LFNKGIYPPIAGLPSLSRLMKDGIGEGRTRKDHGQVAAQLFASYAKVKRIRALAAIVG
                     EEELSDLDKIYLKFGTAFEEIFLRQAYEENRTIEQTLEIGWKMLSLLPQDELYRIARE
                     DIQRYYMPEAASA"
     gene            complement(11824..>12197)
                     /locus_tag="PLQ77_05600"
     CDS             complement(11824..>12197)
                     /locus_tag="PLQ77_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10183.1"
                     /translation="HRLEQVVKLVGSDALPPAEQYILFCAESIKNAFLQQNSFDPQDK
                     FCSPEKQLKILASLLLLYKKGLERVRAGVSVKEIAALEEASRLVRLKSEIPNQDIGKI
                     DEFMNRLEQKLDLLAAPSKGA"
BASE COUNT         2712 a         3514 c         3285 g         2686 t
ORIGIN      
        1 atcgtatttc aacggcctaa gcaacgcctt tttaaaatgt atttgcaatt tgccccttcc
       61 tgtaatacta acggcgcgaa taaccccggt tatgagctca atcaccgcat tcaaggagac
      121 gctatgctga aatttttccg caaacacgcg cgaggctggt tcatgctggt cttcatgggc
      181 atcatcattt ttgttttcgt cctgtatttc ggaacggatc gaagcatgca gaccgccaac
      241 gctctggccg tcgttgacgg ctatgtggtc accgaatccg aatattacaa tgaatactcc
      301 aaactgacgg atatgatcag agcgcagtac ggcgcggcgc tgacggcgga catgctcaag
      361 cagatggacc tgaaaaaaat cgcctatgac aatctgatca accgccagat catcatcgcc
      421 agggccaaag acatgaaaat gcaggtctcc gaagacgagc ttcggcagat gatcatggcc
      481 atgcccgccc tgcagacgga cggccggttc gacaactaca aataccagca gcttttgcgc
      541 ttcaaccgga tgaccgccga agattttgag gccggacaga aaatcaccct ggcggccgga
      601 aaaatagaag ccattatccg cgaaggcatc aaggtctccg accgggaggt gctggatctc
      661 tacaccctgc agaaccagaa gatcaatctc ggcttcatcg ccgtcgccgg cgccgacgtc
      721 gcgggaaaaa tcaatcccgc gaccccggat ctggaagagc acctcaagaa aaacagcggc
      781 gcgtttcgcg ttgccgaaca ggtcaaaatt caatacctgc actttgcggg cgaggccttc
      841 gcgccggccc aaatcaccgc ctccgacatt cgggaccact acaaccgcca caaggacagc
      901 tacaagggca aagacggaaa gccactgtcg ccggagcagg ccgccccgat cattgcccgg
      961 gagatgaagc agagccgcgg actgcaggat gcctacctgg aagccaaaaa ggcgcacgac
     1021 accatttacc aggagcaaaa tttcgaagac tacgcggcaa aacaccaact gaaaacccag
     1081 acggttgatt ttttcccgct caacaagccg ccgcaggcgc tcgcttccgt caaggatctg
     1141 gcccaggagc tggccgccct ggagcgaaag gacatcagca aagtcatgag cgacgccaac
     1201 ggctatttcg tgatccgtct ggatgacaaa aaagccgcct atacgccgcc gctcaaagcc
     1261 attgaaccgg aagtccggca agcctacctg aaaagcgagc aggacagaat ggccgcccag
     1321 gaggccgaaa gcctgcttgc aaaactgcgc aaaggggaaa gcatggagaa actggcgggg
     1381 gaaaaggggc tgagaatcca ggaaaccgga ttcttccagc ccggcggcgt cattcccaaa
     1441 ctgggcgcga atcccgacgc cgtgaatgtg ctgatgtccc tgtccccgag ccgcccctac
     1501 ccggaaaagc ccatggtcat cgatcattcc catgtcattt tcaggctcaa agacgtaagt
     1561 gcgctggaca tgaacgattt cgaagccaaa aaggaaatct acaggaaagt cgccatgaac
     1621 ctgaagcggg aagaagcgat gaaaacatgg ctggagggaa acaaagaggc gctgatgaaa
     1681 gaaaagcgat tgaaaattca aaaagagctg aaggatttat aatcgatgcg cctctatggc
     1741 gccgaagcgg ccgcgcccat gaaattttct ttccgaccgg acgaaagaaa aacatgggtg
     1801 gcaaagatga aaaaagggaa gaaccgcggt tcttcccttt tttatgtttg gtggagctga
     1861 ggaggatcga actcctgacc tcttgaatgc cattcaagcg ctctcccagc tgagctacaa
     1921 ccccaataga ttccccgaga attcgcctgc cccttaacat gcccctcccc ggctgtcaac
     1981 agtttttcag cttgactttt ggccttccgt ctatataatc gcgcccgtgc cggagtggtg
     2041 aaactggtag acgcagagga ctcaaaatcc tccgcccgca agggcatccc ggttcgattc
     2101 cgggctccgg caccatatct aatgccttga tctctcctgt cttaggactt tccagcagcg
     2161 ggccacccag agaaaaaagc agcgcctcat cgggaaaggt tattgttagc tgcaattgtt
     2221 aaatcctttt tacgccacca tggctaataa atgtagagtc tttgaataat tacggtttat
     2281 tgataaactt tatcgtatta tttgcaggaa gcggccctga aaagaattta accgtccagc
     2341 ttttcttttt cgccgaaccc ttttggaaaa attttctttc ggtcaggaaa agcgcagagg
     2401 tcatttgcaa aaatgcgaag cgagagttat gcattcaggc cggagaggta aaataattgg
     2461 gcgggagggg agttcccgac ttgccggacg ataaccgagg aaggacgctc tttttctcct
     2521 aagacggaga gatgtgatga aaaatgtgat catgttttta tgcgcggttc tcgcgcttgg
     2581 atgtttcccg gtttctgtcc aatcggcgat tgcggcggaa ccttctgttt acgtggatta
     2641 cgaatacggc gatctgaaag aggtgattgt cggcctgccg tacggcatgt cgccgtcttt
     2701 ggaggcaaaa tggtttgccg acgcgctgaa agtactgccg ccagatgaag cggattacgc
     2761 ccggaagacg gcggggatgc tctggacgga gatgatcaat cctgaaacgg ggaaaagcga
     2821 gacgcgaatg ctggaggagg aaaatcaggc ctttatcggg atattgaagt ctctgggcgt
     2881 caaggtttac cgtccgacgg agatcaccgt cgattttatc aaggaacatt acggcgcgga
     2941 tgttctttta aacggtttca gccaggactt cccccgcgac aacatggccg tcatcggcac
     3001 aaacgtcatc gaattcaatt tgcgcacgcc gattcgcaag gcggacatat ccggattccg
     3061 gaatattctg tccgaaaaat gttccgaccc ggcggtcaaa tggttttcca tgccgcacac
     3121 ggagctgctt aaacccgccc gcccggacac gccgtttctc gagggcggcg acgtcatcgt
     3181 tctgggcaag accgttttag tcggcaatac gcaaaatccc agcgtgggat ccaatgaggc
     3241 aggttgccgg tggctgaaaa atattttggg cgatcaatat caggtgaaac gcgttcggct
     3301 tgtagaaagc gtgctgcatc tggattgcgt gctttccgta ccgcgcgagg ggctcgccat
     3361 catctgcgaa gaagcctttg tcgacggcct gcccaaggag ctcaagggct gggacctgat
     3421 cagagtgagt ctcaaagatg ttcagcggct ggccgtaaac ggcgttcccg tcaattccga
     3481 aaactacatt ttgtcgtata accggcacaa tgacaaccgc gccatccagg ccgagctcga
     3541 aaagcggggc atcagagtcc accgcgtgtt tttcggaacg cacaatgggc aggggggatc
     3601 gctgcgctgc gcgacccagc cgctgaagcg aaaggtcaga atgaagaaag caaacggcgg
     3661 gtgaacggaa gcaacgcatc gattccgtcc tccgccgccg gtgaaatcga ccggttgcgg
     3721 cgtttagcgc cacaaccctt tttcggcttt ttgcaaattg cagagttttt gtaattgtcg
     3781 cgccatccgc agtctcggca aatccctact caaagctgac agcggttttc atggcgcggt
     3841 tggcctcttt gttcaagttg acctctatca tttcgcggta tcgctcaagc ggaaacttgt
     3901 gagtcagcag atcatccacg cgtattttcc gggatgaaat taattccagc gccatttgat
     3961 acgtatgctt tccgccattc gccgtatgat tgtaccggta gccatagcaa cctttgacgg
     4021 tctgaagctt gagccacaat gtggtcaggt ccagtttgac ttccttgccg atgccgatga
     4081 cggaaagcgt gccgtttgtc gtcaggacgc gcagcgccaa attcagggtg tcggcatgac
     4141 ccacggtgtc ataaatcttg tcgaagcctc ccatgaggac gcgctctccc agcagcggct
     4201 gataggcatg ggcgcccgtg atttccacgg cggcgtcgat gattccgccc cggatggtcc
     4261 gatgggcgcc gcaggccttc aaatgctccg aggcaaaaga agacggttcg accaccgtaa
     4321 tatcgcagcc gatatccagc gcccgcatgc tcttaaccac catggcgccg atgacgccgc
     4381 cgccgatgat cagcaccttg tccgtatccc gcggccggtt gtccagcaca gcctgcaggc
     4441 ccaccgacag cggctccacc agggccgccg attccgtgga aacgccgtcg ggcacaaggt
     4501 gcacctggct tttgtgcgca acgaaaaact cggcaaatcc gccgttgatg tctttgcaga
     4561 tgccgatgaa catcccggga gagaaagcgc cttcagcgaa gttttcgcaa ttggccgttg
     4621 tccccgccgc gcaactccgg caaacgggcc ggatcgcccg tgcataacag ttgagcgcgg
     4681 ggaccacgac gacccggtcg ccgtttttca aatgggaaac atccgcgccg gtttccacga
     4741 tctcgccgca gatctcgtgt cccggaacgc agggaaagga ggtaaagggc gatgccgtcg
     4801 gggaatcctt caggaaaatc agattcatgt cgctgccgca aactccgcag agccgcgttt
     4861 ttatcttgac ccactcaggc gctggaagtt tcggctccgg catttcgaca agcttcaccg
     4921 tggcaaaggg gccgctgtag cagaagcttg cgctgatcgg ccgcagcgcc tggatgatca
     4981 gaaatttcgg cacattcacg ttaaattgca gcgcttgcat caaaggcctc cttgccggtg
     5041 ttctgattga ggcgctattc catcttcagc gactgataaa atagaaccga tgccggactg
     5101 gtcacggtcg gatcggtcat cacattcaca caggccggtt ttccggattg aaaggcccgg
     5161 ttgagcgcgg gaataatgtc ttcatctttg gcggcaaact cgccgtgtcc gcccaatcct
     5221 tcgaccattt tttcgtagtg ccgtgttccc agttccgagc actggagccg atcacagccg
     5281 atggacattt cctgggtatg cttgatcatg ccccaggcgc agtcgttgtt gaccacgcag
     5341 acaaccggga tgttgtgccg caccatggtg tcgaattcca tggcattaaa gccgaaagag
     5401 ccgtcgccgt tgaaaagcag gaccttcttg tccggatgcg ccagtttggc ggccatggca
     5461 aacggaatgc cggtgccgag gcagcccaga aggcccgaag actgactgat gacgccggcc
     5521 tgatgcctgg acataaaccc gaccaggccg aaataagatg tatccccgcc gtcaatgaca
     5581 tagaaggcgt catcgccgac acagctttga atttgctcgg ccagacggat tggatggatg
     5641 ggatcggatg ccgtctggcg aattttcagt tctccgtcga tcatggagcg gcttgccgcc
     5701 ctcagcaggg tcacccattc accgcggtcc tttttgacga gcaggggatt gagctgcccc
     5761 agcacggaac cgatatcgcc gaccagtccg gcatcggcct gccggttccg gtcgatttca
     5821 tgcgggtcga tatcaatccg cacaaccttg gtggcgggcg gaataacggc gcccgactgc
     5881 aaaagccagt tcaggcgaat tcccgcaatc acgatcacat ccgcctgggc aatggcgacc
     5941 atcaagccta cgttgccgca gtcccagacg gaaaggggat ggttgtcggg aagcgtcccc
     6001 cggccgttat tgagcaggat aaagggcatc cccgttttat caatgaattg cccgagacaa
     6061 tcctcacagg aactgaaccc cacgccggtt ccgccgataa acaatggctt tgcggcattg
     6121 ttgatcagat ccgccgccgc gctcaagtcg gcgtcgtcgg ggtggacggt gtatttccgc
     6181 gtggaccggc tgggcatcgg cgccagggac tcatcaacct tgatattgag gatatcgggc
     6241 ggcagctcca gcatcaccgg accgggcctc ccctcaacgg cctgccggaa agcgatcgcc
     6301 agatattccg ggatccgctt gatatcgtaa caggtggccg cccatttggt gatcggcttg
     6361 acgacatcca gctgattcat ttcctgaaga gctcccctca gatcgtccct cagcgggtgc
     6421 ttgccgcaaa tcaccaccat cggcgcattg tccaggcagg cgttcgcaat cccggtgagc
     6481 ccgttggtaa acccgggccc cgtggtgaga aaacatacgc cgggtttgcc ggtataaaca
     6541 gaccaggcat gcgccatcat cgccgctgcc tgctcgtgcc ggacgtcaat ggtgcgaatc
     6601 ctgtattccg taaaactgtc cagaatcgac tccagatgcc cgccggaaat gccgaacacg
     6661 atttcgacct tttccacctc tttcaaatat ttcccgacca gatgcccgcc attgatcatt
     6721 cccatcgtcc gtctccttat tttcagttca tgaccagttt cacaccatca ttaaaccact
     6781 gaacataggc tgccatgccg tctattttca gtttcccgcc ggccgccgcc agaaaagtct
     6841 gttcatcact gcccgacgac atcacctgga aagcggtgcc ggaatccgac cagacaagcg
     6901 ccgcgtcata cgaatgcccg cacccggaca gggtggacag cttgccccgg tcaaaaacga
     6961 acagccttcc gtgcttcccg tcggccgttt gaatcatgat tttaacctgc atgccgccaa
     7021 gaaaacttcg gtatgccgcg tttttcgccg cggccttctt cagtttttgg tacaggatga
     7081 aaagcaagag tcttaatttc atcatcatct cccgatttga taatggtcct tcacccacgc
     7141 gccgattttt tccatggctt ccggatagga taaccgcgtg cgccagccta actccgtgcg
     7201 ggccagcatg gtatccacgt cattgtccct gcccagcacc cccgcgctca gcctggtcag
     7261 gggagcgcgg atatgaaggg gattgagcac ggcttccaaa acgaagccgg tcatccacgc
     7321 cagccggaaa ggaatgcttc ccctggggcg cttcccgatc catgagccga gatccgtgat
     7381 gtactctttc cagtttacgc gccagtcgtc gcgaaaatga tacgccttcc cgcaggcaat
     7441 gtccccggcg gcggccagga tcatgccgtc caccagatga tccacataca caaagctggc
     7501 gctgtaccgc ccgccatcga tgaggggcac gccggtcgcg gacatcatgc gatcggcaat
     7561 gtcccgcacc cacacacttc ccggaccgac cacattggcg gggcgcacga tggtgcattt
     7621 catcttgtct tcacgatgaa aggcgcgcac caatgcctcc gcgtcaactt tcgcgtcgtt
     7681 gtaggaaact ccggatttcc gcgggggatc ggattccttc acgcctttga ggtgacggcc
     7741 cattcccatg gccgcgatgg agctgacata aacaaaacgt gatgccctgc ctgccgcttc
     7801 ctgcaaaagg tttcccgtgg catcacagat ggccgtgtaa aattgccgct tcgtgcccca
     7861 gtccgtcaca cgcccggcca ggtgaaaaac cgtttcaatg ccttcgcaga ccccgcgcag
     7921 actttccgga tcggtcaggt ctccccgaat aacccgcaca ccctgttttt tcaagacatc
     7981 ggcattttcc ccgggaagca ccagcgcccg gacggaactg ccgcgagcca gcaaatctct
     8041 caccagaacc gagcctatga atcctgtagc gccggtcaca agggcattca tatcgttcct
     8101 ttccgatgta ccgggaccgg caggcttccg gcggaagatt ttatctcgac cggcgtcagc
     8161 gcgccctttt cccgggtgag caaatcggcg tcattgccgc gggagtggag gggaaacagc
     8221 tccggccgaa ttcctttgtt ttgcgtgcct ttcatgatgt ccgcactaat gaatttttca
     8281 tagagattgc cgcacaacgg atcccgtacg gcctgaagtt ccgtgccgat atccagaaga
     8341 aaagccgcct ggcccgcatc cgtgaaattt attttggaaa cttgatcgca cgtttttgaa
     8401 tattttcaaa aaaaggcggc gattacgaat ccatttcttc tgaaatatct cgaataatca
     8461 tgccgcttca aataattcgt tcccgtgcaa atagcaagtt aaacttttat ttgcacagca
     8521 gccgcgccgt tgcttttcaa tcccgcggcc gttgaccgat ctcacccttt cccgcgtcta
     8581 tcgctttgaa tccccggcgc gcctcccgcg ggtcaatcct ccagaagcat tcgctcctga
     8641 taataatgcg tgatatcgta cgccgcgtag tccatgagca gtatgatgcg catcaggctg
     8701 tccacggcct gctgatgcgc cagggcgttg acgaaggttc gctgaaattc gccgtgcagc
     8761 cacatctggc ggcgcatgac gttcagggca taaatggcct cgtgcagggg aatgccctgc
     8821 tggcggcagg tctccgcata ctgccgaaag aattcgcggc ctttttcaaa gcgttcggga
     8881 tcgaaaagaa gggtcctcag gcggctgtaa aatttgatcg cctgcgggat caatttatcc
     8941 ttggggatgc cgtgataaaa aaccgtctgc ttgtccttcc gcacggtcgc ggcccagttc
     9001 gaggcaattt ccacaacatt ggattccagg atatccaaga gcctgatcga ttccagcttg
     9061 tctctcttgg ccagctcctg atattcccgg gtcacgtcat aggaggcgta atcgaagagc
     9121 aaaatggtgc ggttcagggt gtccagcgcc tgctgctggt tgatcagcga gctgaagatc
     9181 atctgaaact cggcatagag ccagatgtgc cgccgcatca aaatcagcgc atagaccatc
     9241 tcggccatgg gaatgcccag ggcgtagcat tccgtggcgt agattctgaa atatttctgg
     9301 acctcttccg tgattttttc gtgaacaaac attttgctga agttccgata gaactgaacg
     9361 cactggaaaa tgattttctg ttcgttcaga tttttatagg tcggcgtttt ggcgttgttt
     9421 tgcacatcca gggcccatcg tttggccatt ttctccgcat gctgttccgc cagttccaca
     9481 tatttcaggg tgtaggttct catcatccat ctcctttctt ttatggattc ggccttgatt
     9541 ccatcatatt ctgctctggc cgcgctgccg gatgagcttt ttcacaaaga cctcttcccg
     9601 ctccatttcc tcgatcctgt ccgtgatgta ctttttcgac tcctcatgct ggggaataaa
     9661 aatcttctcc agggcgttga cgcggcgctg cacctttttc aattccctgg agagcatcat
     9721 gacggacttg gtcatggtcg catattcggc aagcgtaagc agcgttttgc gggactggtc
     9781 tcttgcttcg tcgtaggcgg cggtggtttg gggcagcgta aaggcgggat ggggattttt
     9841 gagcgtaagc cgaatcaccg gcattcgaag gcccatgagt ttggcataat ccgtccggag
     9901 gaaatagctt ctcacttcct tgcagctctg cagggaggcc acatcggagc ccatatccac
     9961 ggccgcgccg cgataggccc cgtagagcct gttcaacccc tgctgaaatt cagcctcaat
    10021 cctgcggatt tcgccgatgt tgcggacgat ctcgatggcc agaatttcgc gcttctggtt
    10081 gagaaggtcg tacccttcat aggcaaacgc caggtcttcc ttgagcctgc gcagggaaga
    10141 tttggtcggg ggcaattcta ttttcggcat aatttcatca ccggcttgcc atcgtttaag
    10201 tccctccctc cacgggaggg gttggaggag ggtgaaaata acattccccc tcccctcaat
    10261 ccccgcccgc cgggggcggg gaagttctca tcagggaacg gctggtggcc tcgcgccacc
    10321 tcgcgccacc ttcaggtggt taggtgttag gtaatcgtga ccgttcccta catcccccga
    10381 ttccgggtcg agcgtcgcgg gcccagagta acatcataac gaaattctct ttacgcgctt
    10441 gcggcctccg gcatgtagta tcgctgaatg tcttccctcg cgatgcggta cagttcgtct
    10501 tggggaagga gcgacagcat tttccagccg atctccagcg tctgctcgat cgtgcggttt
    10561 tcctcgtagg cctggcgcag aaaaatctcc tcaaaagccg tcccgaattt caggtagatc
    10621 ttatccaggt cggaaagctc ctcctctccg acaatcgccg ccagcgcccg aatgcgtttg
    10681 actttcgcgt acgaggcgaa aagctgggcg gccacctgcc cgtggtcctt tcgcgttctg
    10741 ccttctccga ttccgtcttt catcagcctg gacagagaag gaaggccggc aatgggcgga
    10801 tagattccct tgttgaaaag agcacgctcc agaacgatct gcccttcggt gatatagccg
    10861 gtcaggtccg gcacgggatg ggaaatgtcg tcgctgggca tcgtcagaat cggaacctgc
    10921 gtgatggatc ccttgccgcc tttgagcctt ccggcgcgtt cataaacgga ggccagatcg
    10981 ctgtagaggt agccgggata tcccttgcgc gaaggaatct cgccgcgcga actggatagc
    11041 tcccgaagcg cttcgcagta attggtcatg tcggtcagaa tgaccagaac gtgcatccct
    11101 tcttcaaagg ccagatattc cgccagcgtg agcgccgtgc gcggggtcac cagccgctcg
    11161 atggaaggcg aatcggccag gctcaaaaag atggcggagt gcgtgagcac gcccgattct
    11221 tcgagcgaat cgatgaagaa ccgggccacg tcgaacttga cgcccatggc ggcaaaaatg
    11281 acggtgaatt cctcctcggc ggtgagaatc ctggcctgcc ttgcgatctg cgcggcaatc
    11341 cggttgtgcg gcatgccgct gcccgagaaa atcggaagct tctgcccccg aatcagcgtg
    11401 ttcattccgt caatcgccga gatgcccgtc tgaatgaagt cccggggata ttcccggctc
    11461 cagggattga tcggcaggcc gttgacgttc atcatccggt cggtgatcac ctgcggcgcg
    11521 ccgtcgatgg gccgccccag gccgtcatac acacggccca gcatgtcgcg gtccactgga
    11581 atctcaaggg cacggcccac aaaggtaacc cgggtgcctt ttacggaaag cccgctggtg
    11641 ccctcataca cctggacgac ggctctttgt tcatccacct ccagcgtctg gccggtgcgc
    11701 agctcgcctg aagcgtcccg gatttccacg atatcgccgt agccgacccc ctgaatgccg
    11761 tcgacaaaaa tcaacggacc ggcaattccc gacaggttgg tgtattcgat ttccgcggac
    11821 atattacgct cctttcgacg gcgctgcgag caagtcaagc ttctgctcca gccggttcat
    11881 gaactcgtcg atttttccga tgtcctgatt gggaatttcc gatttcagcc gcaccagacg
    11941 cgacgcctct tccagagcgg caatctcctt cacgctcact ccggcgcgca ctcgttccag
    12001 gcctttttta taaagaagaa gcaggctcgc caggattttg agctgctttt cagggctgca
    12061 gaatttgtcc tgggggtcga aagagttctg ctgcagaaag gcgttcttga tcgattcggc
    12121 gcagaaaaga atgtattgtt cggcgggcgg cagcgcgtcg cttccgacca gcttcaccac
    12181 ctgctcgagc cggtgat
//
LOCUS       DAOPAF010000045        12181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5171_length_12181_cov_40.124361, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000045 DAOPAF010000000
VERSION     DAOPAF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5171_length_12181_cov_40.124361"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLQ77_05605"
     CDS             <1..521
                     /locus_tag="PLQ77_05605"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPL10184.1"
                     /translation="TIGAVGVRELSPMANLDFLVVPLGTYLSNHLKFGNRLIVKPLVF
                     ATNYFLKEDGKFLNEKVDKKVWLMWMEGRVHGEYKAIETPIGYIPHYEDLAKLFVQIF
                     GKKFSREMYDKLFAVRVEKFLEKLVRVEKAYRDEENIPDEFHAILDMLRNRLTAAKAK
                     AGQSVITPGEFE"
     gene            complement(682..1983)
                     /locus_tag="PLQ77_05610"
     CDS             complement(682..1983)
                     /locus_tag="PLQ77_05610"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPL10185.1"
                     /translation="MTPTTTGLIGFAVLIILMFLRMPVGFVMALVGYLGFGVLVSWSA
                     ASSLVIRDFYSTFSSYSLTVIPLFVLMGQIAFHSGISGRLFNAAHKFFGHLPGGLAIA
                     TIGACAGFSAVCGSTSATAATMASVALPEMKKYKYDPALATGVVAAGGSLGILIPPST
                     IFIIYGIMTEQSIGELFMAGVVPGILLSCFFVLSVLIWAYFRPEIGPRGPKSTWTEKF
                     KSLSGLIETLLLFVLVMGGLFAGFFTPTEAAGIGAAGTILIALIGRHLTWKGFKNALY
                     DTTRITCMIFVVVAGATVFGHFLAVTRIPADIGTFVSGLELPPMVIMGIIIVIYLLLG
                     CLMDSLAMIMLTIPIFYPVVLTLGFDPIWFGVIIVVVSGMGVITPPVGINAYVVAGVA
                     RDVPLHVIFKGTLHMLYAMIALAVLLMVFPKIATFLPQMLK"
     gene            complement(1980..2465)
                     /locus_tag="PLQ77_05615"
     CDS             complement(1980..2465)
                     /locus_tag="PLQ77_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPL10186.1"
                     /translation="MFSPAKYLTRAGKAFNILACAAVIAMMLLSTADVFLRLLGKPIP
                     GTYELVGFLGTIVVSFALAFTSMEKGHIAVEILVEKLPQRAQLAIESFCNLIGALLFG
                     VIACQAVLYALDIRKSGEVSLTLQMPPYPFIFGIAAGCALLCLLLVADFIKSVRRTID
                     A"
     gene            complement(2478..3509)
                     /locus_tag="PLQ77_05620"
     CDS             complement(2478..3509)
                     /locus_tag="PLQ77_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPL10187.1"
                     /translation="MKKNSLLTVISIICAAVFFVFGWTCGVQAQTTLTYSIFFPATHA
                     QAKAGEAWAKEIEKRTGDKVKINIFAGGSLTPADQCFDGVVKGISDIGMSCFAYTRGR
                     LPLLEALDLPMGYPSGMVATRVANDFAKKMKPKELDNVKVLYLHAHGPGLLHTQKPVR
                     TMADVKGMKIRSTGLSAKIVEMLGGVSVAMPQGGTYEALQKGVVQGTLAPMETLKGWK
                     QAQVVKYTTDCTNIGYTTAMFVVMNRNKWNALPKDVQKVFDDVSEEWIAVAGKAWDDA
                     DIEGYKFSAELGNKVIKLSAAEQARWKKAVKPVAEGYIKSAESKGLPGRQAVAEVELL
                     IKKHAKAKK"
     gene            complement(3792..4088)
                     /locus_tag="PLQ77_05625"
     CDS             complement(3792..4088)
                     /locus_tag="PLQ77_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10188.1"
                     /translation="MSLLLGGVVAAVIGLISLIFWWSDFLAILRGALPIFLFLGGALA
                     VYVGFDDIQDKIREERQRQNEELERAREEIESAKAQAERYRDELDRLKDEKKQS"
     gene            4283..4657
                     /gene="hisI"
                     /locus_tag="PLQ77_05630"
     CDS             4283..4657
                     /gene="hisI"
                     /locus_tag="PLQ77_05630"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPL10189.1"
                     /translation="MIEPNFNKTDGLIPAIVQDAQTKDILMLAYMNRASWEATLQSGK
                     ATFWSRSRQKLWLKGETSGHVQIVKEIYIDCDEDTLLLQVEQLGGAACHTGHRSCFFR
                     KREGGDFVVVGEKVFDPGEVYK"
     gene            4654..5526
                     /gene="hisG"
                     /locus_tag="PLQ77_05635"
     CDS             4654..5526
                     /gene="hisG"
                     /locus_tag="PLQ77_05635"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPL10190.1"
                     /translation="MNVLKLGIPKGSLEKNTVDLFQKAGWRITYDSRSYFPDIDDPEL
                     SCSLVRAQEMSRYIEDGHLDLGLTGIDWIMENDSDVVAVQDLIYSKVSTRQARWVLVV
                     RDDSPIRSLADLEGKHISTELVNFTRRYFAEKKIKVGVEFSWGATEAKVVDGLVDAVV
                     EVTETGSTIRANKLRIVHELMKTNTQLIANREAWNDPWKRKKIDQIRTLLTGSLLAMG
                     KVGIKLNVSRANLDRVMSLLPSLKAPTISSLYSEEWFAIESVIDTGVVRDLIPQLLEA
                     GAQGIIEYPLNKVI"
     gene            5541..6128
                     /gene="hisB"
                     /locus_tag="PLQ77_05640"
     CDS             5541..6128
                     /gene="hisB"
                     /locus_tag="PLQ77_05640"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPL10191.1"
                     /translation="MARKAKIVRKTTETDIALDIDLDKSAASRIDTTIPFLNHMLELF
                     ARHGQFKLVVKSKGDTEIDDHHLVEDLGICLGQAVGQALGKKTGINRYGSASVPMDES
                     LCRVDMDICGRPYLIYKVRYERRKIGGFDPALVKEFFKAFTDHSGITLHITLEYGSNS
                     HHIIESVFKAFARALRQAVTINPEISGVLSTKGTL"
     gene            6148..6834
                     /locus_tag="PLQ77_05645"
     CDS             6148..6834
                     /locus_tag="PLQ77_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10192.1"
                     /translation="MILHQVHSKSIVCAMWLLGLLILCPGVPQAREAAKADDKTVARV
                     AVIPFQKVLPEETSSTVQCPICGSVNSSGVIFEGAEKIVEEMATDHLRDYKGVEILPA
                     EKAAGVYRRVSTSLLKQSLLQVFLETGRELKADVLMAGFVYRCRERVGYDYSAERPAA
                     VAFEIHLISVSDGRTLWRGTFDKTQKSLMEDVFQVSSFFRGGGKWLTARQLTKLGVDQ
                     IFKTFPGFER"
     gene            6913..7644
                     /gene="hisA"
                     /locus_tag="PLQ77_05650"
     CDS             6913..7644
                     /gene="hisA"
                     /locus_tag="PLQ77_05650"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPL10193.1"
                     /translation="MLIIPAVDVKNGQCVRLAQGDFNRVTVYAENPADVASRWAQKGA
                     KRIHLVDLDGSVAGLPKNAPVIVEIAQSVPVPVEVGGGIRSMETVNYYLENGVSSVIL
                     GTAAIQDESFVRTAARTHRGKIILGIDALGGEVAVRGWTQKTGENAVELARRYARDGI
                     QAIVYTDIARDGMETGVNVEQTRALAKAVDIPVIASGGVATLADIDALLAVKDCSFYG
                     VIVGRALYTGAIALEDAIAKTQRAS"
     gene            7666..8007
                     /locus_tag="PLQ77_05655"
     CDS             7666..8007
                     /locus_tag="PLQ77_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPL10194.1"
                     /translation="MAECIFCKIIKGDIPSRKVYEDDAVLAFDDISPMAPAHVVIIPK
                     KHIATLMDVDAKADITGKLIAAAQQVAEIKQVAESGFRLAINCNADGGQVVFHLHMHL
                     LGGRKLEDQLG"
     gene            8024..8470
                     /locus_tag="PLQ77_05660"
     CDS             8024..8470
                     /locus_tag="PLQ77_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPL10195.1"
                     /translation="MDIQAKLNEDMVTAAKAKDKIRLSALRMLKTALHNREIDLMRPL
                     NETETMQVLSGLVKQRKDSIEQFAKGGRQDLVEKEEAELKILQDFMPAQMSDEEVETL
                     IQKAIADVGAASVKDMGKVMKVLMPQITGRADGKAAGEKVKALLSR"
     gene            8526..10289
                     /gene="dnaG"
                     /locus_tag="PLQ77_05665"
     CDS             8526..10289
                     /gene="dnaG"
                     /locus_tag="PLQ77_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPL10196.1"
                     /translation="MRFDDSKIEEIKSRVDIVELASEYLTLKKAGRNYLGLCPFHQEK
                     TPSFTVSREKQIFYCFGCGEGGNVITLLMKMANKSFPEAIQYLAEKTGVLLPVRTFGR
                     DSREKDSLHDEILRLNARAAQFFARQLSSPGGRTARDYLQKRAVTEDIVRQFRMGYAP
                     DAWRSLADDIEGGGLSLKMAEQAGLVIAGKEGGGYDRFRGRLMFPIENPFGEIVAFGG
                     RILGEGEPKYLNSPESPVYSKGKNLYGLNKAKEAIRRKGFCLMVEGYFDVISLWNAGV
                     CNVVATLGTALTRDHLELLRRYTQDVVALFDPDTAGRKALDRSLELFLGANMRARALI
                     LPDGCDPDDFVKKHGPARLDELIALAPSISDYYIDTVLGNGRTFEENRDLVREALEFA
                     GKINNEIEKNLFIKRLAEKSGVDQVLLKREARRKAAPAPSGKKASAPTAMNLLELNLI
                     RLMLEYPQRALLVEQENILDCFLENVLKDLGTKILETYKLLGYVDINVLLPSDEDKPL
                     RERLYKMSVEEPPAEDDQVERNFADNMRRIREKWYKEQKRQVQHKLRQAQEGGNQDLI
                     RELTFQKQNLMKEENKYVNKS"
     gene            10307..11920
                     /gene="rpoD"
                     /locus_tag="PLQ77_05670"
                     /pseudo
     CDS             10307..11920
                     /gene="rpoD"
                     /locus_tag="PLQ77_05670"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
BASE COUNT         2987 a         3143 c         3571 g         2480 t
ORIGIN      
        1 cgacgatcgg cgcggtgggc gtccgggagc tttctcccat ggccaacctg gatttcctgg
       61 tggttccgct ggggacgtat ctttccaacc atctgaagtt cggcaaccgc ctgatcgtca
      121 agccgctggt ttttgccacc aactactttc tgaaggaaga cggcaaattc ctgaatgaga
      181 aagtggacaa gaaagtctgg ctcatgtgga tggaaggccg ggtgcacggc gaatacaagg
      241 cgatcgaaac gccgatcggc tatattccgc attacgaaga ccttgccaag ctgtttgttc
      301 agattttcgg caaaaagttt tcgcgggaaa tgtacgacaa gctgtttgcc gttcgggtgg
      361 aaaagtttct ggagaagctg gtccgcgtgg aaaaggccta ccgggacgag gaaaacattc
      421 ccgatgaatt ccacgccatc ctcgacatgc tgcgcaaccg tctgaccgcc gccaaagcga
      481 aagccggaca gtccgtcatc acgcccggcg aattcgaata aacggaagcc ggtaaaacaa
      541 aaaagccccg tcctttccgg tggggctttt tttgtgtttt gcatctcatg acagctgttg
      601 cacggtaaac ctgaaatctc tgaatctttt cccttccgtc tgcagctttc agcctttacc
      661 cttcagcctt cagtcttcag cctatttaag catctgcgga aggaaggttg cgattttcgg
      721 aaagaccatc agcaagaccg ccagcgcgat catcgcgtag agcatgtgca gcgtgccttt
      781 aaagatgaca tgcaaaggca catcgcgggc cacgcccgcc accacgtagg cattgatgcc
      841 gacgggcggc gtgatgacgc ccatccccga aacgacgacg atgatcacgc cgaaccagat
      901 cgggtcaaaa cccagcgtga gtacgaccgg ataaaaaatg ggaatcgtca gcatgatcat
      961 ggccagcgaa tccatcagac agcccagaag caggtagatg acgatgatga tgcccatgat
     1021 caccatggga ggaagctcta gaccggaaac gaatgttccg atgtccgccg ggatccttgt
     1081 caccgccaga aaatgtccga agaccgtcgc accggcgacc acgacgaaga tcatgcaggt
     1141 gatccgggtg gtatcgtaca gagcgttctt gaaccccttc caggtcaaat ggcgtccgat
     1201 caaagcgatc agaatcgttc ccgcagcccc gatgccggcg gcctccgttg gcgtaaagaa
     1261 accggcaaac aggccgccca tgaccagaac aaatagaagc agggtttcga tgaggccgga
     1321 taaggatttg aatttttcgg tccaggtgct tttgggaccg cgcggcccga tttccggacg
     1381 gaaatatgcc cagatcaaaa cggacagaac aaagaagcag gacaggagaa tgcccgggac
     1441 cacgccggcc atgaacagct cgccgataga ctgctcggtc ataatcccgt aaatgatgaa
     1501 aatggtgctg ggaggtatga ggattcccag gcttccgccg gccgcgacca cgccggtggc
     1561 cagcgccggg tcgtacttgt attttttcat ctcgggaagc gccacggaag ccatggtggc
     1621 ggccgtggcg ctggtcgagc cgcacacggc cgagaagccg gcacaggcgc cgatggtggc
     1681 gatggcgagc ccccccggca gatgaccgaa aaacttgtgg gccgcgttga agaggcggcc
     1741 gctgatgccc gaatgaaagg cgatttgacc catcagcaca aacaggggaa tgacggttaa
     1801 gctataggag ctgaaggtgg aatagaagtc gcgaatgacc aggctcgagg cggcgctcca
     1861 ggaaaccagc acgccgaatc ccaggtagcc gaccagggcc atgacaaatc ccacgggcat
     1921 ccgcaaaaac atcaaaataa tcagtacggc aaagccgatc aaaccggtcg tggtgggagt
     1981 catgcgtcaa tcgtcctccg cacggatttg atgaaatcgg cgaccaagag caggcataaa
     2041 agagcgcagc ccgcggcgat gccgaaaatg aagggatagg gcggcatctg cagcgtcagg
     2101 gatacttcgc cgcttttccg gatatccagc gcgtagagaa ccgcctggca ggcgatgacg
     2161 ccaaaaagca gcgcgccgat gaggttgcag aagctttcga tggccaactg cgcgcgctgc
     2221 ggcaattttt ccaccaggat ctccacggcg atgtgccctt tttccatgga ggtgaaagcc
     2281 agggcgaagg atacgacgat ggttcccagg aatcccacca gttcataggt tccggggatg
     2341 ggcttgccca acaggcgcaa aaaaacatcc gccgtggaca gcagcatcat ggcgatgacg
     2401 gccgcgcagg ccagaatgtt gaacgctttg cccgcgcgcg tcaggtattt tgccggactg
     2461 aacatgaatc ggtcctttta tttttttgcc tttgcgtgtt tcttgatcag gagctccact
     2521 tcggcgacgg cctgccgtcc gggcaaacct ttgctctcgg cgcttttgat gtagccttcg
     2581 gcgaccggct tgacggcctt cttccatcgg gcctgctccg ccgcggacag tttgattact
     2641 ttgttgccca gttcggcgga gaatttatat ccttcaatat ccgcatcgtc ccaggccttg
     2701 ccggccacgg caatccactc ctcactgacg tcgtcaaaca ccttctgcac gtctttgggc
     2761 agagcattcc atttatttct gttcatgacg acgaacatgg cggtggtgta gccaatgttg
     2821 gtgcaatcag tcgtgtattt gacgacctgc gcctgcttcc atcccttcag ggtttccatg
     2881 ggggccagtg tgccctgcac aacgcctttt tgcagcgctt cataggttcc gccctgcggc
     2941 atggccacgg agacgccgcc cagcatctcc acgattttag cggaaagccc cgtggacctg
     3001 attttcatgc ctttgacatc cgccatcgtc cggacgggct tctgtgtatg cagaagcccc
     3061 gggccgtgtg cgtgcagata cagaaccttg acgttgtcca gttctttggg cttcatcttt
     3121 ttcgcgaagt cattggccac gcgggtggca accatgccgc tggggtagcc catcggcaga
     3181 tcgagtgctt ccagaagcgg caggcggccc cgcgtatagg cgaagcagga catgccgatg
     3241 tcggaaattc ccttgacaac gccgtcaaaa cactgatcgg ccggggtcag cgaaccgccg
     3301 gcgaagatgt tgattttgac cttgtccccg gtgcgttttt caatttcctt tgcccaggct
     3361 tcgccggctt ttgcctgcgc atgcgtggcc ggaaaaaaga tgctgtatgt cagggttgtc
     3421 tgcgcctgaa cgccgcaagt ccacccaaaa acaaaaaaca ctgccgcaca gatgattgaa
     3481 atgactgtca ataagctatt ctttttcata taacccgttt cctccgatat ccccaatgtt
     3541 tccatcaata gatacatatc cgcgttcttt ggttgatgct ggttttggat ttgcgctggt
     3601 gcttttggaa aagcctgctt acctgaagga atcgggcaag caggcatcaa tacatgtagg
     3661 tataataagg cgagaacgga tttactgtaa aaaatgcact catgttaagt ccataacgat
     3721 gtattgaatg ttgctcttat gtgattttac cggcggtgtc aagatgtatt tgtgcggtgc
     3781 gccgccgtgt ttcagctttg cttcttttca tcctttaacc ggtccagttc atcgcggtag
     3841 cgttcggcct gggctttggc ggattcaatt tcttccctgg ctcgctccaa ttcttcattt
     3901 tgccgctgtc tttcttcccg tattttgtcc tgaatatcgt caaaaccgac atagacggcc
     3961 agagcgcctc ccagaaaaag gaagatgggc agcgcacccc ggagaatggc caggaaatca
     4021 gaccaccaga aaatcaaact gatcagtccg atgaccgcgg caacgacgcc tccgagcagc
     4081 aaagacataa gcccaatcct ccgccaataa agttaaatcc ttttaatacg atgctgacgg
     4141 tttttttatc atgattggaa gacgcaggca agccggaaat caacgaaacg aaagctaaaa
     4201 tttctcttga tttaatgggg ttcatccatt aaacatctta tttttataag gcggtaaata
     4261 attgaatttg cggaagggac ccatgatcga accaaacttt aataaaacgg acgggttgat
     4321 tccggccatc gtgcaggatg cccaaaccaa agacatcctc atgctggcct acatgaacag
     4381 ggcctcctgg gaggcgacgc tgcagtcggg caaggccacg ttctggagtc gttcccggca
     4441 aaagctgtgg ctgaaggggg aaacctccgg ccacgtccag attgtcaaag agatttatat
     4501 cgattgcgac gaggacaccc ttttgcttca ggtggagcag ctcggcgggg cggcctgcca
     4561 cacggggcat cgttcctgtt ttttccgcaa acgcgagggc ggcgatttcg tggtcgtcgg
     4621 cgagaaggtt tttgatcccg gagaagtata taaatgaatg ttttgaagtt gggaatcccg
     4681 aaagggagcc tggaaaagaa tacggttgat ttatttcaaa aagccggctg gcggatcacc
     4741 tatgacagcc gcagctattt cccggatatt gacgatccgg agctttcctg ctcgctggtc
     4801 agggcgcagg aaatgtcccg ctacatcgaa gacggtcatc tggacctggg gctgaccggc
     4861 attgactgga tcatggaaaa cgactccgac gtagtggccg tgcaggatct gatttattcc
     4921 aaggtttcca cgcgccaggc caggtgggtg ctggtggtgc gggacgattc gcccatccgt
     4981 tcgcttgcgg atttggaggg caaacacatc tcgacggagc tggtcaactt tacccggaga
     5041 tattttgctg aaaagaagat caaggtcggc gtggagtttt cctggggcgc gaccgaggcg
     5101 aaagtggttg acggtctggt ggacgccgtc gtggaggtca cggaaacggg atcgaccatc
     5161 cgggccaaca agctcaggat cgtgcatgaa ctgatgaaaa ccaacacgca gctcatcgcc
     5221 aaccgcgagg cctggaacga tccctggaag agaaagaaaa tcgatcagat ccggacgctg
     5281 ctgaccgggt cgctgctggc gatgggcaag gttggaatca agttgaatgt ttccagagcc
     5341 aatctggacc gcgtgatgag ccttctgccg tcgctgaaag ccccgaccat ttccagcctc
     5401 tattccgagg aatggttcgc cattgaatcc gtcatcgata cgggggtcgt gcgggatctg
     5461 attccccagc ttctggaggc gggagcgcag ggcatcatcg agtatcccct gaataaagtc
     5521 atctaatccg gagggcagcc atggccagaa aggcaaagat cgtccgtaag acaacggaaa
     5581 cggatatcgc gctggacatt gatttggata aatccgccgc cagcaggatc gatacaacca
     5641 ttcccttcct gaatcacatg ctggagctgt tcgcccgtca cgggcagttc aaactggttg
     5701 taaaaagcaa gggcgacacg gaaatcgacg atcatcattt ggtggaagac ctggggattt
     5761 gcctggggca ggccgtgggg caggcgctgg gcaaaaagac gggcatcaat cgctacggat
     5821 cggcgtccgt gccgatggac gagagcctgt gccgcgtgga catggacatc tgcggccgcc
     5881 cctatctgat ttacaaggtc cggtatgaac gcaggaaaat aggcgggttt gatcccgcgc
     5941 tggtcaagga atttttcaag gcgttcacgg atcacagcgg cattaccctg cacattaccc
     6001 tggaatacgg cagcaacagt catcacatca tcgagtccgt cttcaaagcc tttgcccggg
     6061 cgctcaggca ggcggtgact atcaatccgg aaatttcagg cgttctgtcc accaagggaa
     6121 cgctttgaaa gaaagatcag gcgtctcgtg atcctgcacc aggttcatag caaaagcatc
     6181 gtctgcgcca tgtggcttct ggggttgctc attctttgcc ccggcgtccc gcaggcgcgg
     6241 gaagcggcga aggccgacga taaaaccgtg gcgcgcgtcg cggtcatccc ttttcagaag
     6301 gtgctgccgg aagagacgtc ctccaccgtt caatgcccga tttgcggcag cgtcaattcc
     6361 agcggcgtca tcttcgaagg cgcggaaaaa atcgttgagg aaatggcgac cgatcatttg
     6421 agggattata agggcgtgga gatccttccc gcggaaaagg cggcaggcgt ttaccggcgg
     6481 gtatcgacga gtcttctcaa gcagtctttg ttgcaggttt tcctggaaac cggacgggaa
     6541 ctgaaagcgg atgtcctgat ggcggggttt gtgtaccgct gccgggaaag agtgggctac
     6601 gattattccg cggagcgccc cgcggcggtt gcctttgaaa ttcatctgat ttccgtgagc
     6661 gacggccgca ccctgtggcg gggaactttc gacaaaacgc aaaaatctct gatggaagat
     6721 gtttttcagg tatcttcttt cttcaggggc ggcgggaagt ggctgacggc gcgccagttg
     6781 acgaagctgg gagtcgacca gatttttaaa acctttcccg gttttgagcg ctgaccggac
     6841 cggggcgtga atgcccgcgg aaatgctccg gccgatgttt catgaaacgg atccttcaag
     6901 gagtcactca aggtgctgat tattccggcg gtcgatgtta aaaacggaca atgcgtgcgg
     6961 ctggcgcagg gcgatttcaa ccgggtgacc gtttacgctg aaaatcccgc ggacgtggcc
     7021 agtcgctggg ctcaaaaagg ggccaaaaga attcacctcg tggatctgga cggctccgtc
     7081 gccggattgc cgaagaacgc gccggtcatc gtcgaaatcg cgcagtcggt gccggtgccg
     7141 gttgaggtcg gcggcggcat ccgcagcatg gaaacggtca actactacct ggaaaacggc
     7201 gtatcgagcg tgatcctggg gacggcggcc attcaggacg aatcgttcgt ccggacggcg
     7261 gcccgaaccc atcggggaaa aatcatcctg gggatcgacg cgctcggagg ggaggtcgcg
     7321 gtccgcggct ggacgcaaaa gacgggcgag aacgcggtcg aactggcgcg gcgctatgcc
     7381 cgcgacggga tccaggccat tgtctatacg gacattgctc gcgacggcat ggagacgggc
     7441 gtcaatgtgg agcaaaccag ggctctggcg aaagccgtcg atattccggt gatcgcgtcg
     7501 gggggcgtgg cgacgctggc cgacatcgat gccctgctgg ccgtcaagga ctgttcgttt
     7561 tacggcgtca ttgtcggccg cgcgctttac acgggcgcca tcgccctgga agacgcgatt
     7621 gcgaagacac agcgggcttc ataaagccgg aaggaggaaa agcatatggc agagtgcata
     7681 ttctgcaaga tcatcaaggg agacattcct tccaggaaag tttacgaaga cgatgctgtg
     7741 ctggcgtttg acgatatcag tcccatggcg ccggcgcacg tcgtcatcat tcccaaaaaa
     7801 cacattgcga cgctgatgga tgttgatgcg aaagcggaca tcacgggaaa attgattgcg
     7861 gccgcgcagc aggtagctga aataaagcag gtggccgaga gcggattccg gctggcgatc
     7921 aattgcaacg ccgacggggg gcaggtggta tttcatctgc acatgcatct gctgggaggc
     7981 agaaaactgg aagatcagtt ggggtaaaac gaggaaagtg cttatggata ttcaggccaa
     8041 attgaatgaa gacatggtga cggctgcgaa ggccaaagac aaaatccggc tctccgcgct
     8101 tcgcatgctg aaaacggcgc tccacaacag ggaaatcgac ctgatgcggc cgctcaacga
     8161 gacggaaacg atgcaggtcc tttccgggct cgtcaagcag agaaaagatt ccatcgaaca
     8221 gtttgccaag gggggccggc aggatcttgt ggagaaagaa gaagcggagc tgaaaattct
     8281 gcaggatttc atgcccgcgc aaatgtcgga tgaagaagtg gaaacgctca tccaaaaggc
     8341 gattgccgac gtgggcgccg cttccgtcaa ggacatgggc aaggtgatga aggtgctgat
     8401 gccgcagatt accggcaggg cggacgggaa agccgccggt gaaaaggtaa aagcgcttct
     8461 gtcgcggtaa ggcaggttat tcgcgaaacg gactcaaacc atcaaaacag ggcaggcaat
     8521 ccttcgtgcg ctttgacgac agcaagattg aagaaattaa aagcagggtg gatattgtcg
     8581 agcttgcctc cgaatatctg accctgaaaa aggcgggccg gaattatctg gggctttgtc
     8641 cctttcatca ggaaaagacg ccttcgttca ccgtcagccg cgagaaacag attttctact
     8701 gtttcggttg cggggaaggc ggcaatgtca ttacgctgct gatgaaaatg gccaacaaga
     8761 gttttcccga ggccattcaa tacctggcgg aaaaaacagg cgtcctgttg ccggtgcgga
     8821 ccttcggccg ggacagtcga gaaaaggatt ccctgcacga tgagattctt cgcctgaatg
     8881 ctagggcggc gcagttcttt gcccgtcagc tctcctcgcc ggggggcagg acggcccgcg
     8941 actatctgca gaaaagagcc gtcaccgaag acattgtccg gcaattccgc atgggatacg
     9001 ctccggatgc ctggcgctcc ctggcggatg atatcgaagg cggcggtttg tccctgaaaa
     9061 tggctgaaca ggccggtctg gtcatcgccg gaaaagaggg ggggggctat gaccggtttc
     9121 gcggccgcct gatgttcccc attgaaaatc ctttcggtga aatcgtcgcc tttggcggac
     9181 ggatcctcgg agagggggaa ccaaaatatc tcaactcgcc ggaatcgccg gtttacagca
     9241 aagggaaaaa tttatatgga ctcaacaagg ccaaagaggc gatccgccgc aagggttttt
     9301 gcctcatggt ggaagggtat tttgacgtaa tctccctgtg gaatgcgggg gtgtgcaatg
     9361 tcgtggccac gctgggcacg gcgctgaccc gcgatcatct ggagctgctc aggcgctaca
     9421 cgcaggacgt tgtcgccctg tttgatccgg acacggccgg acgcaaggcg ctggaccggt
     9481 cgctggagct ttttctgggg gccaatatgc gcgcccgtgc tttaattttg cccgacggct
     9541 gcgacccgga tgattttgta aaaaaacacg gaccggcccg gctggatgag ctgatcgccc
     9601 ttgcgccgtc catcagcgat tattatattg acactgttct gggaaacggc aggacatttg
     9661 aagaaaaccg cgatctggta agagaagcgt tggaatttgc ggggaaaatc aacaacgaaa
     9721 tcgaaaagaa tctctttatc aagcgcctcg cggaaaaatc gggcgtcgat caggtgttgt
     9781 tgaagcggga ggcccgccgt aaagcggcgc ccgccccgtc cggtaaaaaa gcatctgccc
     9841 cgaccgcgat gaatctcctg gagctgaatc tgatccggct catgctggag tatccgcaga
     9901 gagcgctcct ggtggagcag gaaaacatac tggactgctt tctggagaac gtattgaaag
     9961 acctgggaac gaagatcctt gagacttata aactgctggg gtacgtggac atcaacgttc
    10021 tgctgccctc ggatgaagac aaaccgctgc gggagcgcct ttataaaatg agcgtggaag
    10081 agcctccggc cgaggacgac caggtggagc gaaattttgc cgataacatg cgaagaatcc
    10141 gtgaaaaatg gtataaggaa caaaagcgtc aggttcagca caaactgcgg caggcgcagg
    10201 aaggcgggaa tcaggatctc atccgggaac tgaccttcca gaaacagaac ctgatgaagg
    10261 aagaaaataa atacgtgaac aaatcataac attcgggggc atccaaatgg cgaaagaaat
    10321 acagtccgat gaatttaaaa aactgttatc cctgggggaa gaaaagggtt ttttaaccta
    10381 tgatgacgtg aatgacatgc tgccggcaga tttgaattcc tcggagcaaa tcgatgatat
    10441 tatcatgctg tttggagaaa aaaacatcga catcattgat atggagcagg gggaaaaact
    10501 ggtggtgaaa aagccggtgg aagacgccct gccgtccaaa ctcccggaag ctctcgcgct
    10561 ggttcccggc gtgggaaaaa ccggcgatcc ggtcaagatg tacttgcggg aaatggggct
    10621 ggtttcgctc ctgagccgtg aaggcgaagt cgagatcgcc aagaaaatcg aagagggcgc
    10681 ccgcgaaacc atgtgggcca ttttcagcct gaccgtttcc atcgacgaag tgctcagcat
    10741 cggcgaaaag ctggaatccg gggaaatccg cgtcaaaaat gtggtggaca acatcgaaga
    10801 tgaagaagga ttcatggagg aggatgatca taaagacaga gtgctccggc tgatcgcccg
    10861 aatccgtctt ttcaacgacc gcaataaagt cctgcgcgag aagctcaaat ccaagaccat
    10921 gaaacccaag caaagggaag cattgagcgc ggagctggcg aaaaacacga agaacatcat
    10981 cacgctttgc cggaaaatcc gcttcagcaa gaagcagatg caccgtttta tcgcaaggct
    11041 gaaagtcttc gcggacgaga ttgaacgctc cgaacgcgtg atcaatcatt tcaagaagga
    11101 gtcgggcctg acgattacgc aaatggaaaa ggcctggtcc cgaatgaaga aatccaaaca
    11161 ggacgagcgg caggcggcca ggtccgccaa ggtctccatc gagtggctgc acagaagcca
    11221 ggcgtccgtc acggaggcgc aaaaacggct gcggcgcatt gccaaggacg tgggaattcc
    11281 aaccgccacg ctcaagacgg tggtctattc catagaagac ggcgaagcca aggccgaaat
    11341 cgccaaacgg aagctggtcg aagccaacct gcggctggtg gtcagcattg ccaagaaata
    11401 taccaatcgc ggcctgcagt ttctggacct gattcaggaa ggcaatatcg ggctcatgaa
    11461 agcggtggat aaattcgaat accagcgggg ctacaagttt tccacctatg caacctggtg
    11521 gatccgtcag gccatcacgc gcgccattgc cgaccaggcc cgcaccattc gcattccggt
    11581 ccacatgatt gaaacgatca acaaactgat ccggacgtcg cgctaccttg ttcaggaact
    11641 ggggcgcgaa ccgacgccgg aggaaatcgc caacaaaatg gagtatccgc tggaaaaagt
    11701 gcgcaaggtg ctcaaaatcg ccaaggagcc gatttccctg gaaacgccga tcggcgagga
    11761 ggaggacagt catcttgggg acttcatcga ggacaaaaag atcatgtctc cttcggaggc
    11821 caccatcagc atggatctgg cggaccagac gcgcaaaatc ctgtccacgc tgacgccgcg
    11881 ggaagaaaag gtcctgcgga tgcgcttcgg catcagtgat cggctgggta cggcggtcat
    11941 gggggaagtg tcggtggagg aaaccgtgat cgccgagatg gaagagccgg aagtcgtgga
    12001 agaagagccg aagaaagcaa agcattcttc ccgcaagcgt ccggcaaaat aatattgaca
    12061 agcaccggga ttgggtatat ttcaacgcac cttgatgccg gttaaatccg cgcaaatgct
    12121 caaaaaggcg ggcctatagc tcaattggta gagccaccgg ctcataaccg gtaggtccct
    12181 g
//
LOCUS       DAOPAF010000046        11980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5285_length_11980_cov_44.149686, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000046 DAOPAF010000000
VERSION     DAOPAF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_11980_cov_44.149686"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLQ77_05675"
     CDS             <1..822
                     /locus_tag="PLQ77_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPL10197.1"
                     /translation="GAIFSDLLETDAGRKAPVNTCGIEELRPNRWQPRKNFNDEDQKN
                     LIASVRQSGIIQPIIVRRADSGYEIIAGERRWRAAQAAGLKEVPIVVRKATDAEAAEL
                     SLIENIQREELGPLEEADAYVTLMETFHLSQESVSARVGKDRSTIANAIRLLKLPDRV
                     KAALTEKKITPGHGRCLLSLNAAGEQTAALDVILKKGLSVRETERLVNRLKQKPAEKK
                     TAARDRFMADLEKQLTAKCMARVRLKGSSKKGLIEISYQSMDELNRLARYLLDEL"
     gene            875..2227
                     /gene="dnaA"
                     /locus_tag="PLQ77_05680"
     CDS             875..2227
                     /gene="dnaA"
                     /locus_tag="PLQ77_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPL10198.1"
                     /translation="MESVWDKATKIIQEKVSKQNFDTWIKPIKIVSMEDKCVQLSVPN
                     KFFKDWLMDNYLSMIKNTLHSVIGISVDIDFRLSKDKEKKPEVAALPFEQGKNQPPPS
                     PQRSKYFSFLNSNYTFDRFVVGPSNQFAHAASIAVGKQPAKNYNPLFIYGGSGLGKTH
                     LLNAIGLMTINAHPDLNVMYVSAESFMNEMINSIRYDRMQKFREKYRNIGCLLIDDIQ
                     FLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQP
                     PEIETKVAIIEKKMHEGKMELSPSVTHYIASHVESNIRELEGFLIRISAYSSLTNREI
                     DLDLVKEVLKTLVKHNNKEDVSIEEILKVVAGKMNVKISDMKAHNKNKNLVLARQISM
                     YLARKLTNFSFPDIGQKIGGRDHSTVIYANNKILKNIESDASFKKMIQDIEDSVIHKT
                     "
     gene            2380..3492
                     /gene="dnaN"
                     /locus_tag="PLQ77_05685"
     CDS             2380..3492
                     /gene="dnaN"
                     /locus_tag="PLQ77_05685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPL10199.1"
                     /translation="MEFKVNRNIFLDGIQKTLGIVEKKTTTPILNNVLLKAEASKIKI
                     IATDREISLISDYEADIAENGEITLSAKKLFEMVREIQGDTVHFTKSANNIVKVTCQR
                     ATYKIPGLPADDFPSVADSQQVEFFNIPGSVLKELISKTSFAMAFDESRKNLNGVLLE
                     TSSDGKNHIWRMVATDGHRLAVARAQTADPCPEMAKGIIIPRKGLMEVKKLTDVHENV
                     SVGIHKNMFVVKTENTILKVNLVDADYPDYKRVIPAEKGVSVTLDKELFLHALRRMNV
                     VSSERYSGVIISIAPDKMTLNSTNLDVGEATEEIDITYSGEEMEVGFNVNYVIDAVSA
                     ISIDRVVMEVGVGLKPTLIKPAEGDNYLCIVMPLKI"
     gene            3543..5951
                     /gene="gyrB"
                     /locus_tag="PLQ77_05690"
     CDS             3543..5951
                     /gene="gyrB"
                     /locus_tag="PLQ77_05690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPL10200.1"
                     /translation="MVETYDADSIKVLEGLEAVRKRPAMYIGSTSKEGLHHLVYEVVD
                     NSIDEASAGFCDSITVKIRVDNSIVVEDNGRGIPVDIHKTEGVSAAEVAMTKLHAGGK
                     FNNETYKVSGGLHGVGVSVVNALSRTCELEVRREGIVYRQTYQRGVPDGPLHAVGKSR
                     GRGTTVAFLPDDEIFEETEFSFDVIANRLRELAFLNSGVKIALIDERADKKTEFIYKG
                     GIVSFVEYINRNRKVLHKEPIYVTGAREDCSVEVALQYNDSYLENIFSFANSINTVEG
                     GMHMVGFRAALTRVFNNYASANKIIKDGKESLRGEDLREGLACVISVKVRNPQFEGQT
                     KTKLGNSDVRGLVEGIVYEKIGSYLEENPSVARQILNKCMEAARAREAARRARELTRR
                     KSALEVGALPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKVIVTALGTGIGTDDYDVSKLRYHKVIIMTDADVDGSHIRTLL
                     LTFFFRQMRELIERGYLFIAQPPLYKVVDKKKEFYIQNEEQMKNYILENGVNKVNLFM
                     EDGSPFPVSGSKLLTVIKKITRIKDLLDRFEKEHRDRNVVRIAAGDPTLTDETFQNEG
                     ELLKIAGRTVVALGSSVVADYRIDEDPDHGGYKMIFDIKTNGTIHTTCIDRETFRTPQ
                     FGEIRNLMGQVSVLGEPPYSDRQEEGAAPVILPTAKDLVDHIINLGKKGLTIQRYKGL
                     GEMNPEQLWETTMNPEKRTLLQVGVEDAVVADEIFITLMGDEVEPRREFIYQNAMYVS
                     NLDI"
     gene            5967..8486
                     /gene="gyrA"
                     /locus_tag="PLQ77_05695"
     CDS             5967..8486
                     /gene="gyrA"
                     /locus_tag="PLQ77_05695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPL10201.1"
                     /translation="MERDIHHRQTLVNIEDEMKKSYLDYSMSVIIGRAIPDVRDGLKP
                     VHRRILFAMSEMGNRYNKPYKKSARIVGDIMGKYHPHGDAAIYDTLVRMAQDFSMRYT
                     LVDGQGNFGSIDGDPPAAMRYTESRLARISDEALADLEKDTVDFVPNYDESLTEPSIL
                     ATRLPLLLLNGTSGIAVGMATNIPPHNLKEIINGTIALIQNPKMIVEELMKYIPGPDF
                     PTAGFINGREGILSAYKTGRGIIRIRARALIEKNARNDRETIVITELPYQVNKATLIE
                     KISDLVRDKKISGISDLRDESDREGIRVVVELKRDESSAIILNQLYKFTQMEVSFGII
                     MLAISEGRPQVFTLKAVLEKFIAFRQEVVTRRTQFDLARARERAHILEGYIIALNNID
                     EIIKVIKAAKSPNDAAAALCATFHLSEIQAKAILEMRLQRLTGLERDKIDAEYADVTA
                     LIAKLAGILADPQKVLKVIIEELQEIREKYADERRTEIVASTEDIDIEDMIVEEDMVV
                     TISHTGYIKRNAVSLYKSQHRGGRGKVGMGTKEEDFVEKIFIASTHHYLLVFTNMGKV
                     YWLKVYQIPQAGRAAKGKAMVNLVNLAPGEKVSATLPVKEFVSDKFIVMVTRKGIIKK
                     TDLAAYSNPRAGGIIAISIDDDDELVDVQLTNGDQDIFLATRLGMAIRFKEDDVRDMG
                     RTARGVRGINLDEGDDVIGVDIPAQGTFMLTVSENGFGKCTPIEEYRVQSRGGKGTIN
                     LKTVAKIGNVSGVLQVQGEENIMLISNAGKVIRLKVQEVPVNHRITQGVKLIDLEPEE
                     KLVGVARTTSEGDDKDDDVNGNGGDEANGSGEEKGPPADES"
     gene            8573..9004
                     /locus_tag="PLQ77_05700"
     CDS             8573..9004
                     /locus_tag="PLQ77_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPL10202.1"
                     /translation="MKKYELFDHTADLGLEIYGRTKRELFANAALALFDVMIQDVDLP
                     TKRGGKERVKTRTVQGADAGDLLVNFLRELLYWFNGMGRVLNRVSILSCGSKKLVVQC
                     VVEPYHSERHLIKTEIKAVTYHGLSVEKTKNGWKARVIFDV"
     gene            8997..10439
                     /locus_tag="PLQ77_05705"
     CDS             8997..10439
                     /locus_tag="PLQ77_05705"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPL10203.1"
                     /translation="MSEIKLEKLDDNRWLLPRTGAMRVPGIIYANEKISGLLTRDESA
                     KQVANVAHLPGIVDFSLAMPDIHWGYGFPIGGVAAFDMDDGVVSPGGVGYDINCGCRL
                     LATELTLHDIKDNVKDLTTALYRRIPTGVGSTGYVRLSGKDQKKVLEEGAGWAIKAGF
                     GSGQDLETTEDGGCIGGADPEQVSARALERGKQQLGTLGSGNHFLEIEVVEEIFDEKA
                     AQAFGLRKGQVVVMIHTGSRGLGYQICDDYLALMVKHQHETGITLPDRQLACTYLSSA
                     RGQNYLAAMACGANYAWANRQILMHLTREVFEKSLRVAPRELGMRLVYDVCHNIAKIE
                     SFPLAGVEKKLCVHRKGATRAYPAGHDAVPARYRSVGQPVLIPGDMGAGSYVLVGQEK
                     AMQETFGSACHGAGRVMSRTQAIKSSRGRSISRELAEQGVLVMAGSKGTLSEEMPEAY
                     KNLNDVVGVVHDAGIAKKAARLKSIACIKG"
     gene            10512..10826
                     /locus_tag="PLQ77_05710"
     CDS             10512..10826
                     /locus_tag="PLQ77_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10204.1"
                     /translation="MTEILFDADEDWEKRVLCSDEACIGTIGPDGKCRECGKPYEGAL
                     PESHGQKTNPPPAPKEEQPAVPEDDASGEDWEKRVLCGDEACIGVIGPDGKCKECGRP
                     LQ"
     gene            complement(10897..11697)
                     /locus_tag="PLQ77_05715"
     CDS             complement(10897..11697)
                     /locus_tag="PLQ77_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPL10205.1"
                     /translation="MRKALYPTILVLFLMLCLVSAAFAGPTIDSILKKKELVVGTSAN
                     LPPLSFKAKDGSLKGLDIDLGKMIASAMDVKLRVVELPFNELIPALESGKIDMILSCM
                     TITPERNLRVVYVGPYFVSGQSILTTRDVAMNINSMDDINKSDFSIAVPAGTTTEKIA
                     KALLPKAKMVVAGSTEETLDLLRKKKVKALMADYPYTSVTSFKYQDKGFVANPPFSAE
                     PIGIAIRPDDPLLTNFLENLLSLLKGDGMLDGLNKRWFSDPSWISELP"
BASE COUNT         3128 a         3095 c         3363 g         2394 t
ORIGIN      
        1 ggcgctattt tttcggacct gctcgaaacg gacgcgggcc gaaaggcgcc cgtcaacacc
       61 tgcggcattg aggaattgcg gccgaaccgc tggcagcccc ggaaaaattt taacgacgaa
      121 gaccagaaaa acctgatcgc gtccgtccgg caaagcggca tcattcaacc gattattgtg
      181 cgcagggcgg attccggcta tgaaatcatc gccggggagc gacgctggcg cgcggcgcag
      241 gcggcaggtc tcaaggaagt gccgattgtg gttcgcaaag ccaccgacgc ggaagccgcc
      301 gagctctcgc tgatcgaaaa tattcagcgt gaagagctcg gcccgctgga agaagcggac
      361 gcctatgtta cgctgatgga aacctttcat ctttcgcagg aatccgtctc cgcccgggtc
      421 ggcaaagacc gttccacgat tgccaacgcc atccgccttc tgaagctgcc tgaccgggtg
      481 aaggccgcac tgacggaaaa aaagatcacc ccgggacacg gccgctgcct tctgtcgctg
      541 aacgcggcgg gggaacagac cgccgccctg gatgtaattt taaaaaaagg cctttcggtt
      601 cgggaaacgg aaagactcgt caaccggctg aaacaaaaac cggctgaaaa aaagacggct
      661 gcccgggacc gcttcatggc ggatctggag aagcaactga cggccaagtg catggcccgc
      721 gtccgtttga aaggttcgtc caaaaaagga ttgattgaaa tttcctatca atccatggac
      781 gaactcaatc gcctggcccg ctatctgctg gatgagttat aggcaataaa aatgtatcca
      841 catctgtgga taagctgttt ggaaaaggtt gatcatggaa tctgtctggg acaaggctac
      901 gaaaatcatt caggaaaaag tttcaaagca gaactttgat acctggatca agcccatcaa
      961 aatcgtctcc atggaagaca agtgcgtcca gttgtccgtg cccaataaat ttttcaagga
     1021 ttggctgatg gacaactatc tgtccatgat caaaaacacg ctgcacagcg tgatcggcat
     1081 cagcgtggac atcgatttca ggctgagcaa ggacaaggaa aaaaagccgg aagtcgccgc
     1141 ccttcctttc gaacagggaa aaaaccagcc gccgccttcg ccgcagcgga gcaaatactt
     1201 ttctttcctc aacagcaact acacgttcga ccggtttgtc gtcggcccct ccaaccagtt
     1261 tgcgcatgcc gcttccattg ccgtcggcaa acagccggcc aaaaattata atcctctttt
     1321 tatctacggg ggttcagggc tgggaaaaac acacctgctc aacgccatcg gcttgatgac
     1381 gatcaacgcg catccggacc tgaatgtcat gtacgtttcg gcggaatcct ttatgaacga
     1441 aatgatcaat tccatccgct atgaccgaat gcagaaattc cgcgaaaaat accgcaatat
     1501 cggctgtctg ctgatcgacg acattcagtt tctcgccgga aaggacagga cgcaggaaga
     1561 gttttttcat acctttaaca ctcttcacga ttccggcaag cagatcgtcg tgacctccga
     1621 taagtttccc aaggatattc caaacctgga aggccgtctg cggtcgcgct ttgagtgggg
     1681 gctgatcgcg gacattcagc ctcccgaaat cgaaacaaaa gttgcgatca tcgaaaagaa
     1741 aatgcatgaa ggcaagatgg agctttcccc gtccgtgacg cattacatcg catcgcacgt
     1801 ggagtccaat atccgtgagc tggagggatt tttaatccgg atttcagcct attcttcact
     1861 gaccaaccgg gaaattgatc tggacctggt caaggaagtc ctgaaaacac tcgtcaagca
     1921 caacaataaa gaggatgtga gcatcgagga gattttgaag gttgtcgccg gaaagatgaa
     1981 tgtaaagatc tcggacatga aggcgcacaa caagaataag aatctggttc tcgcgcggca
     2041 gatttcaatg taccttgccc ggaagctgac caacttctcc tttcccgaca tcggacagaa
     2101 aatcggaggg cgcgatcatt ccaccgtgat ttacgccaat aataaaattt taaaaaatat
     2161 tgaatcggat gcctccttta aaaaaatgat tcaggacatc gaggacagtg tcatccacaa
     2221 aacgtaacca gggtctgcac gcctcttcca caggcgccgc ggcctgaagg gattgaaatc
     2281 agggcagaaa caacagtcat ccacatatca acaggcgtta ttactaatac tggttatgta
     2341 taggtaagaa aaaaataaaa gtattaatgg gaggcggcga tggaatttaa agtgaacaga
     2401 aatatttttc tggacgggat tcagaaaacg ttaggtattg tcgaaaaaaa gacaacaaca
     2461 cccattttaa ataatgttct cttaaaggcg gaagccagca agattaaaat catcgccacg
     2521 gaccgggaga tcagcctcat ctccgattat gaggcggaca tcgcggaaaa cggtgagatt
     2581 accctgtcgg cgaaaaagct ttttgaaatg gttcgcgaaa ttcagggaga caccgtccat
     2641 ttcaccaaga gcgccaacaa tatcgtgaaa gtcacgtgcc agcgggccac ctacaaaata
     2701 ccggggctcc ccgcggatga ttttccaagc gtcgcggaca gccaacaggt ggagtttttc
     2761 aacattccgg gatcggtttt aaaggaattg atcagcaaaa catcctttgc gatggcgttt
     2821 gacgagagcc ggaagaattt aaacggggtt ctgctggaaa cttcctccga tggaaagaat
     2881 catatctggc gcatggtggc aacggacggg caccggctgg cggtggcccg cgcccagacg
     2941 gccgatccgt gtccggagat ggccaaggga atcatcattc cccgcaaagg gctcatggaa
     3001 gtgaaaaaac tgacggatgt tcatgaaaac gtcagcgtcg gcattcacaa gaatatgttt
     3061 gtggttaaaa cggaaaacac tattttgaaa gtcaacctgg tggatgcgga ctatcccgat
     3121 tacaagcggg tgattccggc ggaaaaaggc gtaagcgtga cgctggacaa ggaattgttc
     3181 ctgcacgcgc tcagaagaat gaacgtggtg tcttccgaac gatacagcgg tgtgattatt
     3241 tcgattgcgc cggacaaaat gacgctgaat tccaccaatc tggacgtcgg ggaagccacg
     3301 gaagaaatag acatcacgta ttccggtgaa gaaatggaag tcgggtttaa cgtcaactac
     3361 gtcattgatg ccgtttccgc catttccatc gatcgcgtgg tgatggaagt gggcgtcggc
     3421 ctgaagccga ccctgatcaa accggccgag ggcgacaatt atttatgcat tgtcatgccc
     3481 ttgaaaatct aaaattggtt tggatggaat ttcaaagaag ttgaaggagt acaaaggcgg
     3541 ttatggtaga aacatatgat gccgacagca ttaaagtttt agaaggtctg gaagcggttc
     3601 gcaagcgtcc ggccatgtat atcggttcca ccagcaaaga aggccttcat cacctggtgt
     3661 atgaagtcgt cgacaacagc attgacgagg cgtcggcggg gttctgtgat tccattaccg
     3721 ttaaaatccg ggtggacaac agcatcgtcg tggaagacaa cggcaggggc atcccggtgg
     3781 atattcacaa aaccgaggga gtttcggcgg cggaagtcgc aatgaccaaa ctgcacgccg
     3841 gaggtaaatt caacaacgaa acttataaag tctccggcgg tctgcacggc gtcggcgtgt
     3901 cggtggtcaa cgcgctttcc cgcacctgcg aactggaagt gcggcgcgaa ggcattgtgt
     3961 atcgccagac ctatcaacgg ggtgttccgg acggcccgct gcatgcggtc ggcaaaagca
     4021 ggggacgcgg caccaccgtc gcctttctgc cggacgatga aatttttgaa gagacggaat
     4081 tcagctttga tgtaattgcc aaccgccttc gggaactggc ttttctcaac tccggcgtca
     4141 aaatcgcact gatcgatgaa cgggccgaca agaagacaga gtttatttat aaaggcggca
     4201 ttgtttcctt tgtggaatat atcaaccgca accggaaagt gctccacaag gagccgattt
     4261 acgtgaccgg cgcccgggaa gactgttcgg tggaagtcgc tctgcagtat aacgacagtt
     4321 atctggaaaa catcttttca ttcgccaaca gcatcaatac cgtggagggc ggcatgcaca
     4381 tggtcggctt ccgcgcggcg ctgacccgag tgttcaataa ttacgcctcc gccaacaaga
     4441 tcatcaagga cggaaaggaa tcgcttcgcg gtgaagacct gcgcgaaggg ctggcctgcg
     4501 tcatcagcgt gaaggtgaga aatccgcagt ttgaaggaca gaccaagaca aagctgggaa
     4561 attccgacgt ccgcggcctg gtggaaggaa tcgtttacga gaaaatcggg tcctacctgg
     4621 aggaaaaccc gtccgtcgcc cggcagattc tcaacaaatg catggaagcc gcccgcgctc
     4681 gtgaagcggc ccgaagggcc agggaactga cgcgccgcaa aagcgcgctg gaagtcggcg
     4741 cgctgcccgg aaagctggcc gattgccagg aaaaagatcc agccctcagt gaaatttatt
     4801 tagtggaagg agattcggcc ggcgggtcgg ccaagcaggg gcgcgaccgg aaaaaccagg
     4861 cgattctgcc gctgcgcggg aaagtgctca atgtggaaaa agcgcgcttt gacaagatgc
     4921 tgcagaacga ggaaatcaag gtgattgtga cggccctggg aacgggcatc ggcaccgacg
     4981 attacgacgt cagcaagctt cggtatcaca aagtcattat catgacggat gccgacgtcg
     5041 acggatccca catccgcacc ctgctgctga cttttttctt ccgtcagatg cgagagctga
     5101 tcgagagggg gtatttgttc atcgcccagc cgccgctgta caaggtggtg gacaaaaaga
     5161 aggaattcta catccagaac gaagaacaaa tgaaaaatta cattctggaa aacggcgtga
     5221 acaaggtgaa tctgtttatg gaagacggaa gcccctttcc cgtttcagga agcaaactgc
     5281 tgaccgtcat caaaaaaatc acgcgcatca aagacctgct ggaccgtttt gaaaaagagc
     5341 atcgcgaccg gaatgtggtt cggattgcgg cgggggaccc cacgttgacc gatgagacgt
     5401 ttcaaaacga aggcgagctt ctgaaaatcg ccggcagaac ggtggtcgcc ctgggcagca
     5461 gtgtcgtggc cgattaccgg atcgacgaag atcccgatca cggcggctac aagatgattt
     5521 tcgacatcaa aaccaacgga acgatccaca ccacctgcat tgaccgggaa actttccgaa
     5581 cgccgcagtt tggtgaaatc cgcaatttga tggggcaggt ttccgtgctg ggagagccgc
     5641 cctacagcga ccgccaggaa gaaggcgccg cgccggtcat tctcccgacc gccaaggacc
     5701 tggtggacca catcatcaac ctgggcaaga aagggctgac catccagcga tacaagggct
     5761 tgggcgaaat gaaccccgaa cagctctggg aaaccaccat gaatcccgaa aagcgcaccc
     5821 ttctgcaggt cggcgtcgaa gacgcggtgg tcgccgacga aatctttata acgctgatgg
     5881 gcgacgaagt ggagccgagg cgcgaattca tttaccaaaa cgccatgtac gtatccaatc
     5941 tggatattta agaaggaaga aataaaatgg aacgagacat tcaccaccgg caaaccctgg
     6001 tgaacataga agacgaaatg aagaaatcct acctggatta ctccatgagc gtcatcatcg
     6061 gccgcgcgat tcccgacgtg cgcgacggac tcaaaccggt gcatcgccgc atcctgtttg
     6121 caatgtcgga aatgggcaac cgctacaaca agccctataa aaaatccgcc cgcatcgtcg
     6181 gcgacatcat gggcaaatat cacccccacg gcgacgcggc catttacgac acgctggtgc
     6241 gcatggcgca ggacttctcc atgcgctaca cgctcgtgga cggacagggc aacttcggat
     6301 cgattgacgg cgacccgcca gcggccatgc gctacacgga aagccgcctg gcgcgcatct
     6361 cggatgaagc gctggccgat ctggaaaaag acacggtcga ctttgtgccg aactacgacg
     6421 aatcgctcac cgagccgtcg attctggcaa cgcgcctgcc cctgctcttg ctcaacggaa
     6481 cgtcgggcat cgccgtcggc atggccacca atattccgcc ccacaacctg aaggaaatca
     6541 tcaacgggac gatcgccctg atccagaatc ccaaaatgat cgtggaagag ctgatgaaat
     6601 acattcccgg cccggatttt cccacggccg gcttcatcaa cggacgggaa gggattcttt
     6661 ccgcctataa aaccgggcga ggcattatcc gcattcgcgc tcgcgcgctc atcgaaaaga
     6721 acgcccgcaa cgaccgggaa accatcgtca tcaccgaatt gccctaccag gtcaacaagg
     6781 cgacgctgat tgaaaaaatt tccgatctgg tgcgcgacaa gaaaatctcc ggcatctccg
     6841 atctgcgcga cgaatcggac cgtgagggca tccgcgtggt cgttgaactc aagcgcgacg
     6901 agagctccgc gatcattctc aaccagctct acaaattcac tcaaatggaa gtctccttcg
     6961 gcatcatcat gctggcgatt tccgaaggac ggccgcaggt ttttaccctt aaagcggtgc
     7021 tggagaaatt catcgccttc cgccaggaag tggtgacccg ccgcacgcag ttcgatctgg
     7081 cgcgcgcccg ggagcgtgcg cacattttgg aaggctacat cattgcactc aacaacattg
     7141 acgaaatcat caaggtgatc aaggcggcca aaagccccaa cgatgcagcc gcggcattgt
     7201 gcgcgacgtt tcatctgtcc gagattcagg ccaaggccat tctggaaatg cgcctgcagc
     7261 gcctgaccgg actggaacgc gacaagatcg acgcggaata cgcggacgtt acggcgctga
     7321 tcgccaaact ggccggcatt ctggctgatc cgcaaaaagt cctgaaagtg atcatcgaag
     7381 agcttcagga gattcgggaa aaatacgccg acgagcggcg cacggaaatc gtggccagca
     7441 cggaagacat cgacattgaa gacatgatcg tcgaagaaga catggtggtc accatctcgc
     7501 ataccggcta catcaagcgc aacgccgtca gcctctacaa gagccagcac cgcggcgggc
     7561 ggggcaaggt cggcatgggc accaaagaag aagattttgt ggaaaaaata ttcatcgcct
     7621 ccacccatca ttacctcctg gtcttcacca acatgggaaa ggtgtattgg ttaaaagttt
     7681 accagattcc gcaagcgggg cgcgccgcca aaggcaaggc gatggttaat ctggtgaatc
     7741 tggcacccgg cgaaaaagtc agcgcaacct tgccggtgaa ggaatttgtc tcggataaat
     7801 ttatcgtcat ggtgaccaga aaaggcatta tcaaaaaaac agatctggcc gcctactcca
     7861 atccgcgcgc cgggggcatc atcgccatat ccattgatga tgacgacgaa ctggtggatg
     7921 tgcagttgac caacggcgat caggacattt ttctggctac caggctgggc atggcgattc
     7981 gtttcaagga agatgacgtt cgcgatatgg gacggacggc gcgcggcgtc cgcggcatca
     8041 acctggatga aggcgacgac gttatcggcg tggatattcc cgcccaggga acgttcatgc
     8101 tgaccgtgtc ggaaaacggc tttggaaaat gcacgccgat agaagaatac cgcgtgcagt
     8161 ccagaggcgg caaaggaacg atcaacctga aaacggtagc aaaaatcggc aatgtttccg
     8221 gtgttttgca ggtgcagggc gaagaaaaca tcatgctgat cagcaacgcc ggcaaggtca
     8281 tccggctgaa ggttcaggaa gttccagtca atcatcgcat cacccagggc gtcaaactga
     8341 tcgacctgga accggaagaa aagctcgtcg gggttgcccg cacaacctcg gaaggcgacg
     8401 acaaggatga cgatgtcaac ggaaacggag gcgacgaggc caatggctcc ggggaagaaa
     8461 aaggtccgcc ggcggacgag tcgtaaaatc cgaaagcggg gagcggtcca acgccgctgc
     8521 ccgctttcca ttcttccttg tttatccttc cgttcaacgc tataattccc ccgtgaaaaa
     8581 atacgaactt tttgaccata ccgccgatct gggcctggaa atttacggtc ggacgaaaag
     8641 ggagcttttc gccaacgccg cccttgccct gtttgacgtg atgattcagg atgttgacct
     8701 cccgaccaaa cgcggcggaa aggaacgggt taaaacaaga acagtgcagg gcgcggatgc
     8761 cggggatttg ctggtgaact ttctgcggga gcttctgtat tggttcaatg gcatgggccg
     8821 ggtgctgaat cgcgtttcaa ttttgtcgtg cggcagcaaa aagcttgtcg tgcaatgcgt
     8881 cgttgagccg taccattccg agcggcattt gatcaaaacg gagatcaaag ccgtcacgta
     8941 tcacgggttg agcgtggaga agacaaaaaa cggatggaag gcgagggtga tctttgatgt
     9001 ctgagatcaa gctggaaaaa ctcgacgaca atcggtggct gctgccgcgg acgggcgcca
     9061 tgcgagtgcc gggcatcatc tatgccaatg aaaaaatatc cggcctgctg accagagacg
     9121 aaagcgccaa acaggtcgcc aatgtggcgc acctgccggg cattgttgat ttttcactgg
     9181 ccatgccgga tattcactgg ggttacggtt ttcccatcgg cggcgtggcg gcttttgata
     9241 tggatgacgg tgtggtgtcg ccgggcggcg tgggctacga catcaactgc ggctgcaggc
     9301 ttttggctac ggaactgacg cttcacgaca tcaaagataa cgtcaaagat ttgaccacgg
     9361 ccttatatcg gcggatacca acaggcgtcg gctccaccgg ctatgtcagg ctttccggca
     9421 aagatcagaa aaaagtgctg gaagaaggtg cgggatgggc gatcaaagcc ggttttggat
     9481 cgggccagga cctggaaacc accgaagacg ggggatgcat cggcggggcg gatccggagc
     9541 aggtttccgc gcgcgccctc gagcggggaa agcagcagtt gggcacactg ggttccggca
     9601 atcactttct ggaaattgaa gtcgttgagg aaatctttga tgaaaaagcg gcgcaggcgt
     9661 ttggcttgcg caaagggcag gttgtcgtca tgatccacac cggctcacgg ggattaggct
     9721 atcagatttg cgacgattac ctggccttga tggtcaaaca ccagcacgaa accggcatta
     9781 cgctgcccga ccgtcagctc gcctgcacct atttatcctc cgcgcgcgga caaaactatc
     9841 tggcggccat ggcctgcggg gccaactacg cctgggccaa ccggcagatc ctgatgcatc
     9901 tcacgcgcga ggttttcgaa aaatccctga gagttgcgcc gcgcgaactg ggcatgcggc
     9961 tggtatatga tgtctgtcat aacattgcca aaatcgaatc cttccccctt gcgggagtgg
    10021 aaaagaaact ctgcgtgcac cgcaaaggcg caacgcgggc ctatccggcg ggtcatgatg
    10081 cggttcccgc ccgttatcgg agcgtcggcc agccggtgct gattccgggc gatatgggcg
    10141 ccggctctta tgtgctggtc ggtcaggaaa aagccatgca ggaaactttc ggcagcgcct
    10201 gccacggggc ggggcgggtg atgagccgga cgcaggcgat caaatccagc cggggccgtt
    10261 ccatcagccg ggagctggcg gagcaggggg tgctggtgat ggccggaagc aaagggacgc
    10321 tgagcgaaga aatgcccgaa gcctataaaa acttaaatga cgtcgttgga gtggttcacg
    10381 acgccggcat cgcgaaaaaa gcggctcgtc tcaaatccat cgcctgcatc aagggctgat
    10441 gatccgcggc atttgacttt ttgccgctcc cggcataaga agccctatca attcaaaaac
    10501 cggagagcgg catgacggaa attctttttg atgcggacga agactgggaa aaacgggttt
    10561 tgtgcagcga cgaggcctgc atcggaacca tcggcccgga cggcaaatgc agggaatgcg
    10621 gcaaaccata tgagggcgca cttcccgaga gccatggaca gaagacgaat cctccgcccg
    10681 ccccaaaaga agaacagccg gccgtgcccg aagatgatgc atccggcgag gattgggaga
    10741 aaagggtcct gtgcggcgat gaggcctgca tcggcgtgat cggtccggac ggcaaatgca
    10801 aggaatgcgg gaggcccctg caataaaaat agcgcgccgg ttcctctgaa cccggcgcgc
    10861 tcattccggc gtttctattc ttttcctatc tgcaaattac ggcagttcgg aaatccagga
    10921 cggatcagaa aaccagcgtt tgttgagacc atccagcatt ccatccccct tcaacaggga
    10981 cagcagattt tccaaaaagt ttgtcaagag cggatcatcc ggacggatcg caatcccgat
    11041 cggttccgcg ctgaagggag gattggccac gaagcctttg tcctgatact tgaaggaggt
    11101 cacgctggtg taagggtagt cggccatcag cgctttgacc ttcttttttc tgagcagatc
    11161 aagcgtttct tccgtcgaac cggcaaccac catcttcgct ttgggcagaa gcgctttcgc
    11221 gatcttctcc gtcgtggtgc cggcgggcac ggcaatgctg aaatcggatt tattgatgtc
    11281 atccatggag ttgatgttca tggcaacatc cctggtggtc aaaatggact gccccgacac
    11341 gaaatagggg ccgacataga ccacccgcag attccgctcc ggcgtgatgg tcatgcagga
    11401 gaggatcata tcgattttgc cgctttccag cgcggggatc agttcattga agggaagctc
    11461 cacgacgcgc agttttacat ccatcgccga cgcgatcatc ttgccgaggt caatatccaa
    11521 acctttaagg cttccgtcct tggccttgaa ggacagagga ggcagattgg cggacgtgcc
    11581 gacgacgagt tcttttttct tcaggatgga atcaatggtc ggaccggcaa aggcggcgga
    11641 caccaaacac aacatcagga acaaaaccag aatcgttggg tacagagctt ttctcatgta
    11701 gtttctccta tcagtgaatt tttttgaagc gataccgtct tcccgtcacg catctgcgtc
    11761 ggtaaaatcc gcaaagcgcc cgcaaaagaa atttgaaaaa ttttggggaa tgtatagaag
    11821 ggcggcggtt tcgtgtcaag cgaaaaaagg cttcacggac ggggaagtca tttagatatt
    11881 gacagtctga agaaattaaa ggtaatggaa cgttggattt tcttgaagga agtgccaagg
    11941 tcactggtgg gcctcctgga cttcaaatcc agtgtggggc
//
LOCUS       DAOPAF010000047        11661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5433_length_11661_cov_48.204119, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000047 DAOPAF010000000
VERSION     DAOPAF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5433_length_11661_cov_48.204119"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLQ77_05720"
     CDS             complement(<1..1161)
                     /locus_tag="PLQ77_05720"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HPL10206.1"
                     /translation="MKRILAFSGFTLWAALMFFCAGAGASIIPVTKIRSADLPGGAPA
                     GWALEKKFGAPSLKIAREGDNYYLHLLSQGNSSFGVRTSARVNVKDFPILSWRWKVQK
                     MPVGGDVRKKSADDQALQVYVAFKETGFPAVLNTPVVGYVWDNEAPKGWSGRSTQIGG
                     DKMRYIVLRNKTDKTGQWYTERRNIYEDYKKLFGDINGGEPLGVTTGLQIHINTQRTR
                     TPAEGLIGEIYFSSETKDVVVTEAAKEKMVAATPVLSSPRGKALPVAGFDDDFNRPGC
                     VNISVAFDTNSTQVDAIPKSSVQTIMEYLVKHPENTLVITGHTDSVGSDAFNLELSRR
                     RAQSVKQYLVEQYAIDEHRLIILGAGESRPIADNDTPEGQAQNRRVTIQDCPR"
     gene            complement(1176..1757)
                     /gene="pyrE"
                     /locus_tag="PLQ77_05725"
     CDS             complement(1176..1757)
                     /gene="pyrE"
                     /locus_tag="PLQ77_05725"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPL10207.1"
                     /translation="MSMDSTKARERLGRIILERSFKYSENPPFTLASGRLSNFYFNCK
                     PTTLDPEGMNLIGLVVFDMLEDAPVTAAGGLTLGADPIANALAVISFQKGRPIKSFIV
                     RKEAKDHGTKSAVEGNVAAGERVAILDDVITTGGSTITAIEQARRAGLVVDRVITLID
                     REEGGRENILQHTDQVQSVFTRTQIMALRAKAL"
     gene            complement(1785..2507)
                     /locus_tag="PLQ77_05730"
     CDS             complement(1785..2507)
                     /locus_tag="PLQ77_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPL10208.1"
                     /translation="MAMTTEKQNRFLSVCASVGLHALAAAVLMFGLTGPATVPKAPGS
                     LDFVWASIAAGKDDSLSPVPKALQKKPPAGEGRSLAAEEAQTPQNAPAEIRTAVPAGE
                     NTAAGDAPAPFGSPTAVYAEASGHGILSDAGRAQAVPAMQPVLSSAYPRYRENPAPGY
                     PETARQRGYEGVVLVAVEILADGRVGKAVVRQSSGYAILDHTAVSAVRDWKFVPAKRS
                     GVPYKTWAELPIKFVIHENSHS"
     gene            complement(2507..4909)
                     /locus_tag="PLQ77_05735"
     CDS             complement(2507..4909)
                     /locus_tag="PLQ77_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPL10209.1"
                     /translation="MTKNQKIKSLLQKQLVILDGATGTELQKRGMPAGVCPEIWCLEN
                     PAVIQDIHASYAKAGARVVYTCTFGANRFKLKQFGVKKDAASVNRELALQTRQACGRK
                     ALVAGDIGPTGLFIEPFGPLAFEKAVDAFNEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELGDIFTIASMTFESDGHTLGGTPPEAALITLQSLGADAVGCNCSTGPEQMAE
                     YIAAMKPLATVPLLAKPNAGLPRLEGLKTVFDMDPASFAAGARKLAAAGANLLGGCCG
                     TTPAHIAALAKAVGKKKPASPRRKSIAALSSARSLLSLQDDQPLLVVGERINPTGKKA
                     LQQELLEGKLSIIRRMAQEQEAQGASLLDVNIGLPGIDEAATIKNVIGLLATTTRLPL
                     VIDSSRVETIEAALRLYPGRMLINSISGEKEKLARLLPLAAKYGAMFILLPLTDGSIP
                     KTAEKRREVVRSIYAKVRKAGMTKEDFIVDGLTMAVASDAEAARETLATVAWCNNTLK
                     CKTVLGLSNVSFGMPGRPWLNAAFLAMAQYCGLTMAIANPAGIEFMNVKKAADALVAR
                     EKAPLLFIRHFSEQAPAAPKTDSAAAVSPEEQVTAAILDGDRDRIVPVLEGMLHNGFP
                     ASRIVDDLLIPAIVRVGDLYEKKIYFLPQLMAAAETMKNALALLDPHLKQKAAAQKGK
                     VLLATVRGDIHDIGKNIVALLLRNYGYCVIDLGKDVQDRAIIEAAKKEKPDVIGLSAL
                     MTTTMVHMKEIISLARAQGIRTDFLVGGAVVTRAYAESIGASFAKDGVAAVKAVDELI
                     KK"
     gene            complement(5008..6051)
                     /locus_tag="PLQ77_05740"
     CDS             complement(5008..6051)
                     /locus_tag="PLQ77_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10210.1"
                     /translation="MPKTSEALDIKPGKIPVVLLYNIEPDWTAAEKEEAAGHSTKLGL
                     ALLEAGFPTVFVPVADENLEGRLGFFDPAGHIVFNWCESLPGISHSEWMVAGRLEAMG
                     FTFTGAGSETLALAQDKARVKALLDAACIPTPAWRVFHLGEAVRWERFPAIVKPIHEH
                     CSAGISRDSVALDEGELARRVPFILEAYRQPALVEDFIDGREFHVSVWGNEELTVLPA
                     AEMDFAFFKDVKDRLCGHEAKFIPGSEAYEKIETRLPAPLDPEEQKAMEIVCRAAYRA
                     VGCRDYARMDIRLQDGVFYVLDVNPNADISHDASMACAAEAAGISYGRMGAAIVGMAA
                     RRHPVFSQWALKP"
     gene            6161..6505
                     /locus_tag="PLQ77_05745"
     CDS             6161..6505
                     /locus_tag="PLQ77_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPL10211.1"
                     /translation="MKNTKSMECMRCGRCCLADFTAYVTEEDIGRWKSEGRDDILGVL
                     EREGAVWEGDRLMSRESGTPLGGCRFFAFDGKTFYCAIHETRPAVCRLFEPGSSEICP
                     QYSKKGNTRNHI"
     gene            complement(6496..7401)
                     /locus_tag="PLQ77_05750"
     CDS             complement(6496..7401)
                     /locus_tag="PLQ77_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL10212.1"
                     /translation="MIRKTAGGPGSVRIIWLGTAGMFVTDGKTGILIDPYVSRFGMGR
                     VAFRLPLEPNRPLIQKWAGRFGRDNIAAVIASHSHFDHAADAPYFAIEAHAPLVGTSS
                     TLNIGRGAGMEENKLVEMTSGRTLCFGNFTIKFIESAHGPAVLGRIPYPGTIDKPLIP
                     PGRAADYRLGGVFALLIQHPAGAILHHGSAGFKPQMYDGTHADVILLGIAGRGNTDQY
                     LENVVLKTRARLVLPIHMDNFFKPLENGMSLLPTVRFGEFCRKTENHRLSFTMNTLPF
                     CNEVGILPLDEIPKTQRMLQSKNQI"
     gene            complement(7422..7760)
                     /locus_tag="PLQ77_05755"
     CDS             complement(7422..7760)
                     /locus_tag="PLQ77_05755"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPL10213.1"
                     /translation="MDKPMTYKGYAAKIEYSEKDECLIGRVSNIRDIITFHGDSVKEI
                     KQAFEEAVDHYLDGCAEKNEEPEKPFDEGQVMRMSSALHRMIALAARNENKSVGEWIA
                     EARKKPRDKS"
     gene            complement(7884..8549)
                     /locus_tag="PLQ77_05760"
     CDS             complement(7884..8549)
                     /locus_tag="PLQ77_05760"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPL10214.1"
                     /translation="MRDLIQHEQFELEVLDRLNSGRFLTNLVFGGGTMLRLCHGLDRF
                     SVDLDFWVTKDFDEATYYRKIEKYLLQFYTLADSAHKHFTILFELKSPQFPRALKIEI
                     RKEAKIIKTETCIAYGANSNIQVLIKTVALEEMMKSKIEAFLSRKEIRDAYDIEFLLK
                     KGIALPAENEILGKLLAGLAKLGKKDFSVKLGTLLDASVRQYYIENGFRILEMRLRDK
                     IMP"
     gene            complement(8537..9118)
                     /locus_tag="PLQ77_05765"
     CDS             complement(8537..9118)
                     /locus_tag="PLQ77_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10215.1"
                     /translation="MNRLQELSRKPYFTLGEVAQTFSLQTASARVLCSRYVRQGLLVR
                     FKNNIYTTVWKWEGLTRQDLFTIANVLQVPSYISLMTALAYYDVTTQAQNNYQESVCL
                     KRSVAYNVREAVFSYVKLQNRFYGDFIKKDGVFIATKEKAFLDAAYLFSFGKYKFDVD
                     SLDMKKLELRKLKSLLSVYPNKTKETVKRLCGI"
     gene            9292..9540
                     /locus_tag="PLQ77_05770"
     CDS             9292..9540
                     /locus_tag="PLQ77_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10216.1"
                     /translation="MTENMVVNIFQHQVPGFNGGPIRIKGGKTRGNQIRVDKNGAVRL
                     VGQKLPGKCVFAGTVGTAMMRALLFFRFGSFKRLFEAN"
     gene            complement(9573..>11661)
                     /locus_tag="PLQ77_05775"
     CDS             complement(9573..>11661)
                     /locus_tag="PLQ77_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10217.1"
                     /translation="PDRPLIAFMGNTVNDKTKEGKGDDELSDISKIVDYLAKLSESQR
                     AGLKNVFNNEYKGNLKLTRNPQANDEILLSYGAGDYWGIVNVGNGLNFINEYQGENVD
                     KVTSTAIIIANSEKYLFENIDKPHSPINVLIGSRKFAEGWNCFRVSVIGLINLGKTKG
                     NKIIQIFGRGVRLKGLKSDGKRCDSSHIEDYFALQDNYRDNLKRLETLCVFSLQRSYL
                     ETFTEAVSSELEITKSFEIAVTPSIINLNGKDACFDTYKRDLKIFKLSKTSVDVKKVL
                     LYPMERKIEYEYVLNGKEQKAVINNVSFNLDYRTNRNEDGRNIRNSLKQANVTCRAFI
                     NNQLFKKALIEQADEANLQIYKGGRKLSEIDVDDVLAYIEAIQYKDELPEHDFDFIET
                     VNSKIAEEFVKKVRNKINWHLNSSIYQYEEELKQSSGEIRGDFIEKYTLVKSFKLSKQ
                     EGNKTKQKTEREFNQEIADFTKTIATIKNALIIEKIGNHIYEPLLRENKVVLNDDIKL
                     VPDKLNEGEKKFVKDFAAYMKKKPYKFKNFNVYLMRNVESLKSIGIYLNDDSEVFYPD
                     FIMWLIGKDKVYINFIDPKGQMGTKDFKTEQYKEKVTIADKAKNPTLPNIEKELKRIH
                     KKDFILNSFILLRDSSDLGKVAQPAWKKKNMIDKNIFRLDWHKLDEKENHADLDKLVD
                     GKSYLDWMMVKAI"
BASE COUNT         2421 a         3174 c         3039 g         3027 t
ORIGIN      
        1 ccgcggacaa tcctgaatcg tcacgcggcg gttttgcgcc tggccttccg gcgtgtcgtt
       61 gtcggcgatc gggcgcgatt cgcccgcgcc caggatgatc aggcgatgtt cgtcgatggc
      121 gtattgttcg accagatatt gtttgacgct ttgggcccgc cgcctcgaca gctccaggtt
      181 gaaggcgtcg gaaccgacgc tgtccgtatg accggtgatg acgagcgtgt tttcgggatg
      241 cttcaccaga tattccataa tcgtttgcac gctgcttttc gggatggcgt ccacctgcgt
      301 ggaattggtg tcgaaggcaa cgctgatgtt gacgcagccc gggcggttga aatcgtcgtc
      361 gaagccggcc acgggcagcg ctttccctct ggggctggag agcacggggg ttgcggcaac
      421 cattttctcc ttggcggctt ccgtcaccac gacgtccttc gtctcgctgc tgaagtagat
      481 ctcgccgatc aggccttcgg cgggcgttct ggttcgctgg gtgttgatgt ggatttgcag
      541 gccggtggtg acgccgagcg gttcgccccc gttgatgtcg ccgaaaagct ttttgtagtc
      601 ttcataaata ttgcggcgtt ccgtgtacca ctggccggtc ttgtccgttt tgttgcgcaa
      661 aacgatgtag cgcattttgt cgccgccgat ctgcgtgctg cgtccgctcc accccttggg
      721 cgcttcgttg tcccagacat agcccacgac cggcgtgttc agcacggccg gaaatccggt
      781 ttccttgaag gcaacataga cctggagcgc ctggtcgtcg gccgattttt tgcggacatc
      841 gccgccgacg ggcatttttt ggactttcca gcgccaggaa agaatcggaa agtctttcac
      901 gttgacgcga gccgacgtgc gcacgccgaa ggacgagttg ccctgcgaga gcagatgcag
      961 ataatagttg tcgccttccc tggcgatttt caaagacgga gcgccgaatt ttttctccag
     1021 cgcccagccg gcgggcgcgc cgcccggcag atccgcggag cggattttgg tgacgggaat
     1081 aatggacgcc cccgcgccgg cgcagaaaaa catcagcgca gcccatagcg taaaaccgga
     1141 aaaagcgagg attcttttca tgtccgctgc cgtccttaca gggctttcgc gcgcagcgcc
     1201 atgatttgag tcctcgtgaa caccgactga acctggtccg tgtgctgcag gatgttttcc
     1261 cgtccgcctt cttcgcggtc aatcagggta atcacgcggt ccaccaccag gccggcccgg
     1321 cgggcctgtt cgatggcggt gatcgtcgag ccgcccgtgg tgatgacgtc gtcgaggatg
     1381 gccactcttt cgccggcggc gacgtttccc tcgacagcgc tcttcgtgcc gtggtccttg
     1441 gcctccttgc gcacgatgaa cgatttgatc ggcctgcctt tctgaaagga aatcacggcc
     1501 agggcgttgg cgatcgggtc ggcgcccagc gtcaggcctc ccgccgccgt gaccggcgcg
     1561 tcctcgagca tgtcaaaaac aaccaggccg atcaggttca tgccttccgg gtccagcgtg
     1621 gtcggtttgc agttgaagta aaagttgctc aggcggcccg aagccagcgt aaaaggcgga
     1681 ttttcactgt atttgaaaga gcgctcgaga atgatacggc ccagacgctc tctggctttt
     1741 gtggaatcca tggacataat ttttttcctc cagcacggat caatttacga gtgcgagttc
     1801 tcgtggatta cgaacttgat gggcagctcc gcccatgttt tatacggaac accggacctt
     1861 ttggccggca cgaatttcca gtctctgacc gcggaaaccg cggtgtggtc cagaatggca
     1921 tagccggacg actgccggac gacggctttg ccgacgcgcc cgtccgccag aatttccacc
     1981 gcgaccagca cgacgccctc gtagccgcgc tgccgcgctg tttccggata gccgggcgcg
     2041 ggattctcgc ggtaccgggg ataggcgctg gaaagaaccg gctgcatggc ggggaccgcc
     2101 tgcgcccttc cggcatccga caaaatcccg tgtccggacg cttccgcata gaccgccgtt
     2161 ggggacccga aaggcgccgg cgcgtcgccg gcagcggtat tttcgccggc cggcacggcg
     2221 gtccggatct ccgccggtgc attttgcggg gtttgcgctt cctctgcggc taaagatcgg
     2281 ccctccccgg ccggcggctt cttctgcagg gctttcggaa caggcgacaa agaatcgtcc
     2341 tttccggcag cgatggaggc ccagacaaaa tcaagacttc ccggcgcctt tggaaccgtc
     2401 gcgggaccgg tcaggccgaa catcaggacg gccgccgcca gggcgtgaag accgaccgat
     2461 gcgcaaacgc tcaaaaatct gttttgtttt tccgtcgtca tggccattat tttttgatca
     2521 gctcatccac cgccttgacg gcggccacgc cgtctttggc aaacgacgcg ccgatggatt
     2581 ccgcatacgc ccgcgtcacc acggctccgc cgaccagaaa gtccgtgcgg attccctgcg
     2641 cgcgggccag cgagatgatc tctttcatgt ggaccatcgt tgtcgtcatc agggcggaaa
     2701 gtccgatcac gtccggtttt tcctttttgg ccgcctcgat gatcgcccgg tcctgaacat
     2761 ctttgccgag gtcaataaca caatatccgt aatttctcaa aagcagcgcg acaatgttct
     2821 tgccgatgtc gtgaatatcg ccccggactg ttgccagcag gactttgccc ttttgcgccg
     2881 ccgccttttg cttcaggtgc gggtccagca gggccagggc atttttcatc gtttccgccg
     2941 cggccatgag ctgcggcaga aaataaattt tcttctcgta caaatccccc acccgcacga
     3001 tggccggaat cagcaggtca tcgacgattc gggaggcggg gaagccgttg tgcagcattc
     3061 cctccaggac ggggacgatg cggtcccggt ctccgtccag gatggccgcg gtgacctgtt
     3121 cctccgggga caccgccgcc gccgaatcgg ttttcggcgc cgcgggcgcc tgctcggaga
     3181 aatggcggat gaacagcagg ggcgcctttt ctctggcgac aagcgcgtct gccgcctttt
     3241 tcacgttcat gaattcgatg ccggccggat tggcgatggc catcgtgagt ccgcagtact
     3301 gcgccatggc cagaaacgcg gcgttgagcc aggggcgccc cggcatgccg aaagaaacat
     3361 tggaaaggcc gaggaccgtt ttgcatttca gcgtgttgtt gcaccaggct accgtggcca
     3421 gcgtttcccg cgccgcttcg gcatcggagg caaccgccat cgtcagcccg tccacgataa
     3481 aatcctcttt ggtcattccg gctttccgga cttttgcata aatcgaccgg acgacctccc
     3541 ggcgtttttc agccgttttg ggaatgctgc cgtcggtgag gggcagcaaa atgaacatcg
     3601 cgccgtattt cgcggcaagc ggcagaagcc tggcgagctt ttccttttcg ccggaaatgg
     3661 aattgatcag cattcggccc ggatagaggc gcagcgccgc ttcgatggtt tccactctgg
     3721 acgaatcgat gaccagcggc agccgggtgg ttgtggcgag aagcccgatg acgtttttga
     3781 tcgttgcggc ttcgtcaatg cccggcaagc cgatgttgac gtccagaagg gatgctccct
     3841 gcgcttcctg ctcctgcgcc attcggcgaa tgatggacag tttgccttcc aggagctcct
     3901 gctgcagggc cttcttgccc gtgggattga tcctttcgcc gaccaccagc aggggctgat
     3961 cgtcctgcag ggatagaagg cttctcgcgg agctgagcgc cgcgatcgac tttctgcggg
     4021 gacttgccgg ttttttcttc ccgaccgcct tggccagcgc cgcgatgtga gcgggcgtcg
     4081 tgccgcagca gcctcccagc aggttggcgc ccgccgcggc cagcttcctc gcgccggcgg
     4141 caaagcttgc gggatccatg tcgaagacgg tcttcagccc ttcgagcctc ggcaggccgg
     4201 cattgggctt ggccaggagc ggcaccgtgg cgagaggctt catggcggcg atatattccg
     4261 ccatctgttc gggacccgtg gagcagttgc agcccacggc atccgcgccc aggctctgca
     4321 gcgtaatgag cgccgcttcc ggcggcgttc cgcccagcgt gtgcccgtcg ctttcaaagg
     4381 tcatggaagc gatggtgaag atgtcgccca gctcttttac cgccagcagg gccgcacgcg
     4441 cctcctgaat gtcgatcatg gtttcgatca cgatcagatc gcagcccccg tcgatcaggc
     4501 cgcgcgcctg ttcgttgaac gcgtccacgg ccttctcaaa ggccagcggg ccgaaaggtt
     4561 cgatgaacag gccggtgggc ccgatgtcgc cggcgaccag cgctttccgg ccgcaggcct
     4621 gccgggtttg gagcgccagt tcccggttga ccgaagcggc gtcttttttc acgccgaact
     4681 gcttgagctt gaagcggttt gcgccgaacg tgcaggtgta aaccacccgg gcgccggctt
     4741 ttgcgtagga ggcgtgaatg tcctgaatca ccgccggatt ttccaggcac cagatttccg
     4801 gacagacccc ggccggcatt cctcttttct gcagctcggt gcccgtcgct ccgtccagaa
     4861 tgacaagctg tttctgaagg agacttttga ttttttgatt ttttgtcacg tcaactcctg
     4921 cgggctctgt ttttcgctga gcccttatat catctttgcc cttccagggg gaattttttc
     4981 catgccggca ttcatttctc ttgaacatca aggctttaaa gcccattggc tgaaaaccgg
     5041 atggcggcgg gcggccatgc ccacgatggc cgcgcccatt cggccatagc tgatgccggc
     5101 cgcctccgcg gcgcaggcca tgctggcatc gtggctgatg tccgcgttgg ggttgacgtc
     5161 caggacgtaa aaaacaccgt cttgcagccg aatatccatg cgggcgtaat cccggcagcc
     5221 gacggccctg taagccgcgc ggcacacgat ttccatcgcc ttttgttctt cggggtccag
     5281 cggcgcgggc agcctcgttt cgattttttc gtacgcctcg gagcccggaa tgaacttggc
     5341 ctcgtggccg cagaggcggt ccttgacgtc tttaaaaaat gcaaaatcca tttccgcggc
     5401 gggcaatacg gtgagttctt catttcccca gaccgacacg tgaaattcgc gcccatcgat
     5461 gaaatcctcc accagagcgg gctgccggta ggcctccagt ataaaaggaa cgcgccgggc
     5521 cagctcgccc tcgtccagcg caacggaatc gcggctgatc ccggcggagc agtgctcatg
     5581 aatgggcttg acgatcgcgg gaaacctttc ccaccggacg gcttcgccaa ggtgaaagac
     5641 gcgccaggcg ggcgtgggga tgcaggccgc gtccagcagg gctttgaccc gggccttgtc
     5701 ctgcgcgagc gcaagcgttt ccgaacccgc gccggtaaag gtgaagccca tcgcctcaag
     5761 acgtccggcc accatccact cgctgtggga aattccgggc agactttcgc accagttgaa
     5821 aacgatgtgt ccggccggat caaaaaagcc cagacggccc tcgagattct cgtcggccac
     5881 cggcacgaaa accgtcggga aaccggcctc gagaagcgcc aggccgagct ttgtggagtg
     5941 gccggccgct tcttcctttt cggcggccgt ccagtccggc tcgatattgt acagcagaac
     6001 cacgggaatt ttgccgggtt ttatgtccaa cgcctcggaa gttttgggca tttctttttc
     6061 ctttcagggt agctcattaa ccatattttt tttggaattc caagaatgaa tttacaaacg
     6121 tccgggccgg tggtattttt cgcgccggag aagcggcgag atgaaaaaca caaaatccat
     6181 ggagtgcatg cgctgcggcc gctgctgcct ggccgatttc acagcctacg tgacggagga
     6241 agacatcggg cggtggaagt ccgaaggcag agacgacata ctgggcgtcc tggagaggga
     6301 gggagccgtc tgggagggcg accgcctgat gtcgcgggag tcgggaaccc cgctgggcgg
     6361 ctgccgtttt tttgcatttg acggaaagac tttttattgc gccatccatg aaaccagacc
     6421 cgcggtgtgc aggcttttcg agccgggttc gtccgaaatc tgcccgcagt attcaaaaaa
     6481 gggaaacaca cggaatcata tttgattttt cgattgaagc atgcgctgcg ttttcggaat
     6541 ttcatccaga ggcagaatcc cgacttcgtt gcagaagggc agggtgttca ttgtgaagga
     6601 caaccggtga ttttccgttt tccggcagaa ctcaccgaat cgcaccgtgg ggagcaatga
     6661 catgccgttt tccagcggct tgaagaaatt gtccatatga atcggaagca cgagccgcgc
     6721 gcgggttttc aatacgacat tttccaggta ttgatcggtg ttccctcttc cggcgatgcc
     6781 cagaaggatg acgtcggcat gagtcccgtc atacatttgc ggtttgaagc ctgcgcttcc
     6841 atggtgcaga atggctccgg cggggtgttg gatcagcagg gcaaagacgc cgcccaaacg
     6901 gtagtcggcg gcccggccgg gcgggatcag gggcttgtcg atggttccgg ggtaggggat
     6961 gcggcccagg acggcggggc catgagcgct ttcaatgaat tttatcgtaa agtttccgaa
     7021 acacagcgtc cggccgcttg tcatttccac caatttgttt tcttccatgc cggcgcctct
     7081 gccgatgttc agcgtgcttg acgtgccgac gagcggcgca tgggcttcga ttgcgaaata
     7141 cggcgcgtcg gcggcatggt cgaaatggct gtggcttgcg atgaccgcgg caatgttgtc
     7201 cctgccgaac ctcccggccc atttttgaat gaggggccgg ttcggttcca gcggcagacg
     7261 aaacgcaacc ctgcccatgc cgaaccggct tacgtaagga tcaatcagga tgcctgtctt
     7321 cccgtcggtt acaaacatgc cggcagtgcc cagccagatg atgcggacgc tgcccggccc
     7381 tccggcggtt tttctgatca tcagtttctc atagagctgg ttcatgattt gtcccggggc
     7441 tttttgcggg cttcggcaat ccactcgccg acgcttttat tttcattcct ggcggccaaa
     7501 gcgatcatgc gatgaagcgc ggaactcatc cgcatcacct ggccttcatc aaaaggcttt
     7561 tccggctctt cgtttttttc ggcgcagccg tcaagataat ggtcaacggc ttcctcgaaa
     7621 gcttgtttga tttccttcac cgaatcgcca tgaaaggtta tgatgtcacg aatattggaa
     7681 acgcggccga tcaggcattc atctttttcg ctgtattcaa tctttgcggc atagccttta
     7741 tacgtcatgg gcttgtccat gggtttgcct ccagttgctc cggtacgtct cacattaata
     7801 aatggcacct gtccataaag atgtttttcc tccctcccgt catgatgatc agtgttgtcc
     7861 tcagacctcg aatattcgat ggcttatggc ataattttat cccgcagtct catttccaga
     7921 atgcggaatc cattttcaat gtaatattgt cgcaccgatg catcaagcaa cgttccaagc
     7981 tttacggaaa agtctttctt gccgagtttt gccaatccgg caagcaattt gcccaggatt
     8041 tcattttcag cgggaagggc gatgcctttt tttaacaaaa actcgatgtc gtatgcatcg
     8101 cggatttcct tgcggctgag aaaagcctca atctttgact tcatcatttc ttccagcgca
     8161 acggttttta tcagaacctg aatattggag ttggcgccgt aagcgatgca ggtttccgtt
     8221 ttgatgatct ttgcctcttt gcggatttct attttcagcg ctcttggaaa ttgcggtgat
     8281 ttcaattcga acaaaatcgt aaaatgcttg tgggcggaat cagcaagagt ataaaattgc
     8341 agcaaatatt tttcaatctt ccggtaatac gttgcctcgt caaaatcctt tgtcacccag
     8401 aaatccaaat ccaccgaaaa acgatccagt ccatggcata aacgcagcat ggtgccgccc
     8461 ccgaaaacca agttggtcag aaatcgtccg ctgttcagcc ggtccaggac ttccagttca
     8521 aactgctcat gctgaattaa atcccgcata gtcttttcac cgtttctttt gttttgttcg
     8581 gataaacgct caacagactc ttcagctttc tcaattccag cttcttcatg tccagcgagt
     8641 caacgtcaaa cttatatttg ccgaacgaga aaagatacgc cgcatccaga aaagcttttt
     8701 ctttggtggc gataaatacg ccgtcctttt taatgaaatc gccatagaag cggttttgta
     8761 atttgacata actgaaaacg gcctcccgga cattgtaagc aacagaacgt ttcaggcaaa
     8821 cgctttcctg ataattgttc tgcgcctgcg tggtgacatc ataatacgcc agggccgtca
     8881 tcagggaaat ataagagggg acctgcaata cattggcgat tgtaaacaaa tcctggcgtg
     8941 tcagaccttc ccatttccag acggttgtat aaatattatt tttaaatctg accagcaaac
     9001 cctgccggac ataacggctg cacagtacac gagcggaggc cgtctgcagg ctgaaagtct
     9061 gcgcaacttc gccaagcgta aaatagggct tccggcttaa ttcctgcaat ctgttcatgc
     9121 ttttactaag catatataat tacctttgat gttaattata tatactttgt aatatttggc
     9181 aagcgaaata aagcgcttaa cagggctgag agaaaacgca atagcaacgg ttagttaaat
     9241 atcatggaat ggtgtgtttt gtgcccggaa tcaataaaac gcaaaacttt catgactgag
     9301 aacatggtag taaatatctt tcaacatcag gtgcccggtt ttaatggcgg cccgatccgg
     9361 atcaaagggg gcaaaacccg cggcaatcaa atccgcgttg acaaaaatgg ggcagtccgc
     9421 ctcgttgggc aaaaactccc cggcaaatgt gtttttgccg gcaccgtcgg tacggcgatg
     9481 atgagggctc ttcttttttt tcgcttcggt tcatttaagc gtctctttga agcaaattag
     9541 ttttcgaact aattgtgcct gtcccgttat ttctatatcg ctttcaccat catccaatcc
     9601 aaatagcttt tgccatcaac caatttatct agatcagcgt ggttttcttt ctcatccaat
     9661 ttatgccagt caagcctgaa tatattttta tcaatcatat tttttttctt ccacgcaggc
     9721 tgtgcgacct tacctaaatc tgaactgtcg cgtaacaata taaaagagtt taatataaaa
     9781 tcctttttgt gaatccgctt cagctctttt tcaatatttg gcaatgtagg attcttagct
     9841 ttgtctgcaa tcgtaacttt ctctttatac tgttctgtct tgaagtcctt tgtgcccatt
     9901 tgacctttcg gatcgataaa gttaatgtaa actttatctt tgccaattag ccacatgata
     9961 aaatccggat aaaacacttc tgaatcgtcg ttcaagtaaa tgccaattga tttcagcgac
    10021 tctacattcc gcatgaggta aacattgaaa tttttaaatt tatacggctt ttttttcatg
    10081 tatgccgcaa agtcttttac aaatttcttt tctccttcat tgagcttatc cggcacgagc
    10141 tttatatcat cgtttaaaac aactttattc tctcgcaata atggctcata gatatgattg
    10201 ccaatttttt caatgatcag cgcatttttt attgtggcaa tcgtttttgt aaaatccgct
    10261 atttcttgat taaattctct ctcagtcttc tgttttgtct tatttccttc ctgcttggat
    10321 agtttaaaag atttaaccaa tgtatatttt tcaataaaat ctcctctgat ttcaccgcta
    10381 ctttgtttaa gttcttcttc atattgataa atgctgctgt ttaagtgcca gtttatttta
    10441 ttgcgcactt tcttgacaaa ttcttcagcg attttgctgt tgacagtttc aatgaaatca
    10501 aaatcgtgtt caggtaattc atctttgtat tgaatcgcct caatgtaagc cagaacatca
    10561 tcgacatcta tttctgaaag ttttcttccg cctttatata tttggagatt ggcttcatcg
    10621 gcttgttcga tcagcgcctt tttgaatagt tgattgttaa tgaacgcccg gcaggtaaca
    10681 ttcgcttgct tcaatgaatt tctgatattg cggccatctt catttcgatt tgtcctgtaa
    10741 tccagattaa aactgacatt attaatgact gctttttgct ccttaccatt cagtacgtac
    10801 tcatattcga tttttctctc cattggataa agcagcacct ttttgacatc aactgatgtt
    10861 ttactgagct tgaatatctt caaatcgcgt ttgtacgtat caaaacaagc atctttgcca
    10921 tttagattga tgatgcttgg tgtcacggcg atttcaaatg atttcgttat ttccaattca
    10981 ctgcttacgg cttccgtaaa agtctccaga taactgcgtt gcaaactaaa aacgcacaga
    11041 gtttccaatc gtttaagatt gtctctgtaa ttatcctgta gggcaaaata atcttcaata
    11101 tgactgctgt cacatcgttt cccatcactt ttcaagccct tcaagcgaac accgcgcccg
    11161 aatatctgga taattttatt gcctttggtt ttccccagat taatcaaacc aatgacagaa
    11221 acacgaaagc agttccatcc ctcggcaaac ttccggctgc cgattaagac gttaattggc
    11281 gagtgcggct tatcaatatt ttcaaaaagg tatttttcgc tattggcaat aatgatggct
    11341 gtgctggtta ctttatcaac gttctcgcct tgatattcat tgataaagtt caagccattg
    11401 cccacattga cgatacccca gtaatcgcct gcgccgtaag aaagcaatat ctcatcattg
    11461 gcctgcggat ttctggtcag tttcaggttg cccttgtatt cattattaaa aacattcttt
    11521 aatcctgctc tttgtgattc agaaagcttt gccagataat ctacaatctt ggaaatatcc
    11581 gaaagttcat catcgccttt tccttctttg gttttatcgt tgactgtatt gcccatgaaa
    11641 gcaatcaacg gcctatcggg g
//
LOCUS       DAOPAF010000048        11554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5492_length_11554_cov_46.291938, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000048 DAOPAF010000000
VERSION     DAOPAF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11554
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_11554_cov_46.291938"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLQ77_05780"
     CDS             complement(<1..904)
                     /locus_tag="PLQ77_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPL10218.1"
                     /translation="MKRVLVSDSMAPEVAAILGNTPGIKVDVKTGMKPDELKAAIRDC
                     DALIVRSATKVTAEIIGAAAKLSAIGRAGAGVDNIDIPAASKRGIVVMNTPGGNTVTT
                     AEHAVAMMMSLARRIPQAAASMKAGRWEKSSFMGNECCNKTLGIIGLGRVGSVVADRA
                     IGLKMNVIAHDPFISPEVAQQMGVKMVSLDELYANSDFITVHSPLSKETKHLVNESAF
                     AKMKKGVFLIHCARGGIVSEKALCDALKSGKVAGAAIDVFEDEPTRNMELVGMDHVIC
                     TPHLGASTDEAQTNVAIAIAHQIAA"
     gene            complement(912..2039)
                     /locus_tag="PLQ77_05785"
     CDS             complement(912..2039)
                     /locus_tag="PLQ77_05785"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPL10219.1"
                     /translation="MLNPNFSSGPCSKRPAWSIDALKGAPVGRSHRSALGKERIVKAM
                     NDTRKVLNIPADYLVGILPASDTGAFECAMWSLLGPKPVTVLVWESFSNGWATDVNKQ
                     LKLKPAVVKVDYGKLPDLSKVDWKTDVVFVANGTTSGVKIPNWDWIPADREGLSLCDA
                     TSYAFANKVDWSKIDVLTYSWQKCLGGEAAHGMMILSPKAVERLNSYDPPWPMPKIFR
                     LKKDGKINKAIFEGDVINTPSMLCVEDYLDAMDWAEKIGLDGLVKRSDANLKVVADWV
                     AKTDWIEFLAEDPQTRSNTSVCLSVTHPKVAAMDADAKSKFLKSISSELSKKNVAHDI
                     NSYKEAPAGYRFWCGPTIEEADLKAALAELEKVFTEKVKSL"
     gene            2290..2625
                     /locus_tag="PLQ77_05790"
     CDS             2290..2625
                     /locus_tag="PLQ77_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPL10220.1"
                     /translation="MTENLAKIADLTGAFRLLELVSYQEHSVVSREIIRKPSGTMTVF
                     AFDAGEGLSEHTAPFDALVYLLEGKAEIAIGGKPHPVSEGEMILMPAHKPHALKAITR
                     FKMLLVMIK"
     gene            2643..3830
                     /locus_tag="PLQ77_05795"
     CDS             2643..3830
                     /locus_tag="PLQ77_05795"
                     /inference="COORDINATES: protein motif:HMM:NF008887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HPL10221.1"
                     /translation="MKFEITPGVDWVGKIDWELRKFHGEEYSTHRGSSYNAYLVRDEK
                     TALIDTVWSPFAKEFVNNLDKEIDLNQIDYVIANHAEMDHSGALPLLMHRIPDKPVYC
                     TANGLKSLKGHYHKDWNFQVVKTGDKLSLGKKELIFVEAMMLHWPDSMMEYLTGDAIL
                     FSNDAFGQHIATEHMFNDLVDQSELFEEATKYYANILTPFSPLVTKKINEVVAFNLPV
                     NFICPSHGVIWRDNPLQIAQKYLQWAADYQENQISIIYDTMWDGTRMLAEHIARGIKQ
                     ADDKVTVKLFNISRRDINDVVTEVFKSKAILLGSPTINKGILNAMSALIEMITGLKFK
                     KKKAAAFGCYGWSGESVKILTDHLSKAGFEMIGDGFKAMWQPGDEALAQALAFGKDFA
                     GKV"
     gene            complement(3913..6213)
                     /locus_tag="PLQ77_05800"
     CDS             complement(3913..6213)
                     /locus_tag="PLQ77_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPL10222.1"
                     /translation="MITKAKPTIEVSLEERLRLQKKLQDITNRIHAAQNIAQILIDLK
                     DGILNLFDAYSITLYVVDKPHNEIYSMFLAGSQLKEIRVPINNHSIAGYVANSFNIVN
                     IADAYNLAELKAIDPELTFDASWDKKSGFRTRQILAAPIFYKDQLMGVIQILNKKGGD
                     TKFTEEDIGLVMEIVEVLGVAFYNQQRFQRRRKTRFDFLLTRNLLKEEELEAAWGESR
                     EQKESIERYLMNKYKISKDDIGHSFEEFFRCRFIEYNDKTIIPGDLIANLKKDYLQRE
                     LWVPLEKIDGNVHVIVDDPNNIIKRDAIENLLKTKKVKYDVALSEDILKYINLFFSSS
                     DDEHSFTELLGKMDEESAGSEEEDGADIVAETDSVIMQLVNKIINDASTRRASDIHIE
                     PNVGKKNVEIRFRVDGDCAIYQTVPFNYRSAIISRVKIMSNLDITEKRKPQDGKIKFR
                     RSSGDELELRVATIPTQGGVEDIVMRLLAKSDTLLPLESMGLLKRNYDEMINIINKPY
                     GMILVVGPTGSGKTTTLHAALHVINRPDRKIWTAEDPVEITQYGLRQVQVQSKIGFDF
                     AAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLDM
                     GIDPLNFADALLGILAQRLVRTLCKNCKEQYHATEEEFEEIVESYGREDFAKLGVSYT
                     DDFKLYRAKGCDACDKTGYKGRMGIHELIVATDAIKKLVQKHETVEVIRAKSMAEGMT
                     TLLQDGIQKVLQGITDFNQVRRVCIK"
     gene            complement(6312..8384)
                     /gene="glyS"
                     /locus_tag="PLQ77_05805"
     CDS             complement(6312..8384)
                     /gene="glyS"
                     /locus_tag="PLQ77_05805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPL10223.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEMIRKALTEKRIAFESV
                     HCLATPRRLVLHIADVAPKQADQIVEKMGPARKAAFDENGRPTKAALGFARGQGLEIA
                     ELETITTDKGEYLCARKTIAGQPTADLLPDILKNFLVSIPFRKSMRWSAYDLRFARPI
                     HWLLALYGGKIVPLKVEDIESGNTSRGHRFMSPEAFAVDGFEDYLAKARKHFVIPDPA
                     ERKEFILEEAQKAAAEAGGKLFYTEDLLETVTFIVEYPVIIRGGFDPNYLQIPKEVLT
                     TTMIAHQKYFPVVDNAGKLLPCFIAVSNTRARNPEVVARGNERVLQARLADASFFFEE
                     DKKIPLESRVESLKKVVFHTLLGTSHKKVMRFRKLAVKIASKVKPSVKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALLAGEKPEIADAIYEHYLPIVAGGDLPRTDEGAI
                     VGIADKMDTIVGFFAVGMPPTGTADPYALRRQALGVINIILSRRYELGLNFLIDECLA
                     ALKDVLKKPAEDVKKDVLEFFRGRLQNQLISQSYAYDTVDAVLAADIDQLVLVIEKIQ
                     ALQAFRANPEFEPLSIAFKRIDNILKDFRGGSVDVNLLSQEAEIKLFANFENIGTRVE
                     KGIAEKDFTAALNRLAALRTPVDAFFESVMVMDNDEKIRFNRLSLLADISALFHKIAD
                     FSKIVTAG"
     gene            complement(8401..9273)
                     /gene="glyQ"
                     /locus_tag="PLQ77_05810"
     CDS             complement(8401..9273)
                     /gene="glyQ"
                     /locus_tag="PLQ77_05810"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPL10224.1"
                     /translation="MTFQELIFALEQYWHSQGCVIQQPYDMEVGAGTFHPATCLRALG
                     PEPWNVAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPVNIQELYLNSLKSFGID
                     PLEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGFDCKPVCAELTYG
                     TERIAMYIQGINNVYDLQWTDKIKYGDVHHKGEVEFSTYNFEVADIPMLRRLFDMYEE
                     EALRIAEKNLPLPAYDYCLKCSHTFNLLNARGAISVAERTSYIGRVRNLARVSAELYM
                     KQREELGYPLLQNH"
     gene            9636..10793
                     /locus_tag="PLQ77_05815"
     CDS             9636..10793
                     /locus_tag="PLQ77_05815"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL10225.1"
                     /translation="MLNFTFHNPTKVLFGKCTICQLAGEIPPGARVMVAFGAESARKY
                     GVLDEVLRALAGFDVTEFGGIEANPEYDTLCSGIALAREKKIDFLLAVGGGSVIDGTK
                     FMAAAIPFAGEPYQLMENKGAAIRQALPLGAVLTLPASGSEMNNRAIISRRAVNWKRA
                     AMNDWFFPRFAVLDPTKTCTLPPMQTANGVVDTFVHVLEQYLTYPVQGKVQDRLAEGL
                     LLLLIEEGPKALVEPENYDVRANLMWASTLGLNGLIGAGVPQDWAAHRAGYELTVLYG
                     LDHARTLAVLVPAMLQVRAGGKKEKLLQYGERVWGISSGAEDVRITAAIEKTKAFFGS
                     MGLPTSLSGCGIEKVDADRFIELLEEHGLTAMGERGEVTPAMMREILALCA"
     gene            10810..>11554
                     /locus_tag="PLQ77_05820"
     CDS             10810..>11554
                     /locus_tag="PLQ77_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPL10226.1"
                     /translation="MHSILPEIGIAVLAATAMGFLFQLCRQPVILGYLVAGALIGPQI
                     GFSLVSHPDNIEVISEIGLILLLFIIGLELNPAKLLSSGKKLIYAGVGQFIFCVLIGL
                     GFFVLLGYPLGGGRIEALYLSLFCALSSTAIVVKLLYDKFELDTLPGRISLGILIFQD
                     LWAILILALQPNFMDPKLHLVALALGKGVLLLAVGFLLSRFALRWIFAKIAKTPEMVV
                     ATSIAWCAFMAGLGSWIGMSMEMGALIAGA"
BASE COUNT         2333 a         3110 c         3379 g         2732 t
ORIGIN      
        1 aggcggcgat ctggtgggcg atggcgatgg ccacgttggt ctgcgcttca tcggtagagg
       61 cgcccagatg cggcgtgcag atcacatggt ccatgccgac gagctccatg tttctggtcg
      121 gctcatcttc gaatacgtcg atggcggcgc ccgcgacctt tccggatttc agggcgtcac
      181 agagcgcttt ttcgctgaca atgccgccgc gggcgcagtg aatcagaaaa acgccctttt
      241 tcattttcgc aaacgccgat tcgttgacca gatgctttgt ttctttggaa agcggggaat
      301 gcacggtgat gaagtcggaa ttggcgtaca gttcgtccag actgaccatt ttcacgccca
      361 tctgctgcgc cacttccgga gaaatgaacg gatcgtgggc gatgacgttc atcttcaggc
      421 cgatggcgcg gtcggccacg accgatccga cgcgccccag cccgatgatg cccagggttt
      481 tattgcagca ttcattgccc atgaaactgg atttttccca gcggcccgct ttcatcgagg
      541 cggcggcctg cggaattctg cgggccagcg acatcatcat ggcgacggca tgttcggcgg
      601 tggtgacggt gttgccgccg ggcgtgttca ttacgacaat gccgcgcttg ctggcggcgg
      661 gaatgtcaat gttgtccact cccgctccgg cccggccgat ggcggaaagc ttggcggccg
      721 ccccgatgat ttcggccgta accttcgtgg ccgaacggac aatcagggcg tcgcagtccc
      781 gaatggctgc cttcagctcg tcgggcttca tgccggtttt gacatccacc ttgatgcccg
      841 gcgtgttgcc cagaatggcc gcaacttcgg gcgccatgga atcgctgact aatacccgtt
      901 tcatagcgtt cttacaaact tttgaccttt tcggtgaaga ctttttccag ttcggccagc
      961 gccgccttta aatccgcttc ttcaatggtc ggaccgcacc agaaacggta gccggccggc
     1021 gcttccttgt aggagttgat gtcgtgagcg acgttcttct tgctcagctc cgaggaaatg
     1081 cttttcagga acttgctttt ggcgtccgca tccattgccg cgactttcgg atgcgtcacg
     1141 gacaggcaaa cagatgtgtt cgaacgggtc tggggatctt cggccagaaa ttcaatccag
     1201 tcggttttgg ccacccagtc ggccacgact ttcaggttgg cgtcgcttct tttgaccaga
     1261 ccgtccagac cgattttctc cgcccagtcc atggcgtcca gatagtcttc cacgcacagc
     1321 atggagggcg tgttgatgac gtctccttca aagatggcct tgttgatttt gccgtctttc
     1381 ttgaggcgga aaattttcgg catcggccag ggcgggtcat aggaattgag gcgctcgacg
     1441 gctttcgggc tcagaatcat catgccgtgc gcggcttcgc cgcccagaca cttctgccag
     1501 gaataggtga gcacgtcgat tttgctccag tcgaccttat tggcaaacgc gtagctggtg
     1561 gcgtcgcaaa gcgagaggcc ttcacggtcg gccggaatcc agtcccagtt gggaattttc
     1621 acgccgctgg tggtgccgtt ggcgacgaaa accacatcgg tcttccagtc caccttggaa
     1681 aggtccggaa gtttgccgta gtcaactttc acgaccgcag gcttcagctt cagctgtttg
     1741 ttgacgtccg tcgcccagcc gttggagaag ctttcccaga ccagcacggt aaccggtttg
     1801 gggcccagaa gcgaccacat ggcgcattcg aaagcgccgg tatcggaagc cggaagaatg
     1861 cccaccaggt aatccgccgg aatgttcaaa actttgcgcg tatcgttcat ggctttgacg
     1921 atccgttctt ttcccagcgc ggaacggtgt gagcggccga ccggcgcgcc tttcagcgcg
     1981 tcgatgctcc acgctggcct cttgctgcag gggccggaac taaagttggg gtttaacatg
     2041 ggtaaccatc cttaaaataa attttcttaa tgctggcaag ttcataccag cattaagcgc
     2101 ttttctcaag aaatttaaac cgtaaaagac ctcccgtgac cgggagtccg gacggttggg
     2161 ggcgaaagca cccgaaaaaa taaaataaca ctttttgact taagtcaaat acattatagt
     2221 tcgcgcaggt tattaatcca tcaaacaaga tgcggtcttg acggacgtac tgaaaaaagg
     2281 agggaaaaga tgactgaaaa tttggccaaa attgcggatc tgaccggcgc ctttcgtctt
     2341 ttggagctgg tttcctacca ggagcactcc gtggtcagcc gggaaatcat ccggaaacct
     2401 tccggaacca tgacggtttt tgcctttgac gccggcgagg gattaagcga gcacacggcc
     2461 ccttttgacg cgctcgttta tctgctggaa ggcaaggcgg aaatcgctat tgggggaaag
     2521 cctcacccgg tgagtgaagg ggagatgatt ctcatgccgg cgcacaaacc gcatgcgctc
     2581 aaggcaatta cccgtttcaa gatgctgctg gtcatgataa aataaaaaac aggaggtgtt
     2641 ttatgaagtt cgagattacg ccgggtgtag attgggttgg gaaaatcgac tgggagctgc
     2701 gcaagtttca cggcgaggaa tactccacgc atcgcggctc gtcgtacaac gcgtacctgg
     2761 tgagagacga aaagaccgct ctcatcgaca cggtctggtc gccttttgcg aaggaattcg
     2821 tcaacaatct tgacaaagaa atcgacctca accaaatcga ctacgtcatc gccaatcatg
     2881 ccgagatgga tcattcgggg gctttgccgc tcttgatgca ccggattccc gacaagcccg
     2941 tatactgcac ggccaacgga ttgaaatccc tcaaggggca ctatcacaag gactggaact
     3001 tccaggtcgt gaaaaccggc gacaagctgt cgctgggcaa aaaggagctg attttcgtgg
     3061 aagccatgat gctccactgg ccggattcca tgatggagta cctcaccggc gacgcgatcc
     3121 ttttttccaa cgacgccttc ggccagcata tcgccactga gcacatgttc aatgacttgg
     3181 tcgatcagtc cgagcttttt gaggaggcga cgaaatatta cgccaacatt ctcacgcctt
     3241 tttcgcctct ggtgacgaag aagatcaacg aagtggtggc gttcaacctg ccggtgaatt
     3301 tcatctgccc cagccacggc gtcatctggc gcgacaatcc cctgcagatt gcacaaaaat
     3361 atctgcaatg ggcggcggac tatcaggaaa atcagatttc aatcatttac gacacgatgt
     3421 gggatggtac gagaatgctg gccgaacaca ttgcccgcgg aatcaaacag gccgacgaca
     3481 aggtgacggt gaagcttttc aatatctccc gccgcgacat caacgatgtg gtgaccgaag
     3541 tcttcaaatc gaaagcgatc ctgctgggct cgcccacgat caacaaaggc attctcaacg
     3601 ccatgtcggc gctcatcgaa atgatcacgg ggctcaaatt caagaaaaag aaggccgcgg
     3661 ccttcggctg ctatggctgg agcggcgaga gcgtgaagat tttaaccgat catctgtcca
     3721 aagccggctt tgaaatgatc ggcgacggct tcaaagccat gtggcagccg ggggatgaag
     3781 cgctcgcgca ggcgctggcg tttggaaagg attttgcggg gaaagtataa tttttgctaa
     3841 acaaaatcaa aaaaagggtg gaagccgctt tgcgttccgc ccttttttta tggatgctgg
     3901 atcgttttac gcttatttga tgcagacccg gcggacctgg ttgaaatccg taatgccctg
     3961 cagcactttc tggattccgt cctgaagcag cgtggtcatc ccctcggcca tggatttggc
     4021 gcggatgacc tcaaccgttt catgcttttg caccagtttc ttgatggcgt cggtggccac
     4081 gatcagttca tgaatgccca ttcggccctt gtagccggtt ttgtcgcagg cgtcgcatcc
     4141 tttggcgcga tacagcttga aatcatccgt gtaggaaacg cccagtttgg cgaagtcttc
     4201 gcggccataa ctttccacga tttcctcgaa ttcctcctcg gtggcgtgat actgttcttt
     4261 gcagttcttg cacagcgtgc gcaccaggcg ctgggcgaga atgcccaaca gggcgtccgc
     4321 gaagttcagc gggtcgatgc ccatatccag gagccggacg atggtttccg gggcgctgtt
     4381 ggtgtgcagg gtgctgaaga ccagatggcc ggtcagcgaa gcttcgacgc ccgtcttggc
     4441 tgtttcaaaa tcgcgcattt cgcccaccat gatcacatcc gggtcggcgc gcagaaacgc
     4501 ccgcatggct gccgcgaagt caaagccgat tttggactgc acctggacct ggcgaagccc
     4561 gtattgcgtg atttccaccg ggtcttccgc ggtccagatt ttccggtccg gccggttgat
     4621 gacgtggagc gccgcgtgca gtgtggtggt tttgccggag ccggtcgggc cgacgaccag
     4681 gatcatgccg tagggcttgt tgatgatgtt gatcatttca tcataattgc gtttgagcag
     4741 ccccatggat tccagcggaa gcagggtgtc gcttttggcc agaagccgca tgacaatgtc
     4801 ttccacgccg ccctgcgtgg gaatggtggc gacgcgaagc tccagttcat ccccggaaga
     4861 gcgccgaaac ttgattttgc cgtcctgtgg ttttcttttc tccgtgatat ccaggttgga
     4921 catgatcttg acgcgcgaga tgatggcgga ccggtagttg aaggggacgg tctggtaaat
     4981 cgcgcagtcg ccgtcgacgc ggaaccggat ttccacgttt ttcttgccga cgttgggttc
     5041 gatgtgaatg tcggacgccc ggcgggtgga ggcgtcgttg atgattttat tgacgagctg
     5101 catgatcacg ctgtcggttt ccgcgacgat gtctgctcca tcctcctctt cagagcccgc
     5161 gctttcttca tccatcttgc ccaggagctc cgtaaaggaa tgctcgtcgt cgctggagct
     5221 gaagaacagg ttgatgtatt tcaggatgtc ttccgacaga gcgacgtcat acttcacttt
     5281 cttggttttc agaagatttt cgatggcgtc tcttttgatg atgttgttcg gatcatcgac
     5341 gatcacgtgg acattgccgt cgattttttc cagcggcacc cacagttcgc gctggaggta
     5401 gtctttcttc aggttggcga tcaaatcgcc ggggatgatg gttttatcgt tgtattcaat
     5461 gaaccggcag cggaaaaact cttcgaagga gtggccgata tcgtctttgg agattttgta
     5521 cttgttcatc aggtaccgct cgatggattc tttctgctcc cgcgactccc cccaggcggc
     5581 ttccagttct tcttccttga gcaggttgcg ggtcagcaga aaatcgaacc gcgttttgcg
     5641 gcggcgctgg aagcgctgct ggttgtaaaa ggcaacgccc agaacctcga cgatctccat
     5701 gaccagtccg atgtcttcct cggtaaattt ggtgtctccc ccttttttgt tgagaatttg
     5761 aatcacgccc atgagctgat ctttatagaa aatcggggcg gcgagaatct gcctggttcg
     5821 gaatccggac tttttgtccc agcttgcgtc aaaggtcagt tcgggatcga tggccttgag
     5881 ttccgccagg ttgtatgcgt ccgcgatgtt gacgatgttg aaggagttgg ccacgtagcc
     5941 ggcgatgctg tgattgttga tcggaacgcg gatctccttg agctgagatc ccgccagaaa
     6001 catggaatag atttcgttgt gcggcttatc gacgacgtac agcgtgatgg agtaggcgtc
     6061 aaacagattc aggatgccgt ctttcaggtc gatcaggatt tgcgcaatgt tctgcgcggc
     6121 gtggatgcgg ttggtgatgt cctgaagctt tttctgcagc cgcagccttt cctccagact
     6181 cacctcgatc gtgggtttcg ccttcgtgat catccggtta tctctccatt ttcgttttat
     6241 tcataaaaat cgcgtacatc ctagcaagcc gcgcggggaa tgtcaaccaa acgaatttgg
     6301 cgccgggcgg atcagccggc ggtgacgatt ttcgagaaat cggcgatttt atggaacagt
     6361 gctgagatgt ccgccagcag cgacaggcgg ttgaagcgga ttttctcgtc gttgtccatg
     6421 accatgacgg actcgaagaa agcgtccacg ggggtgcgca gagcggccag ccggttcagc
     6481 gccgcggtga aatccttctc ggcgatgcct ttctccacgc gcgttccgat gttttcaaaa
     6541 ttcgcgaaca gcttgatctc cgcttcctgg gaaagcaggt tgacgtccac ggaaccgccg
     6601 cggaaatcct tcaggatgtt gtcgatccgc ttgaatgcga tggagagggg ttcgaactcc
     6661 ggattggccc ggaaagcctg cagcgcctga atcttttcga tcaccagaac aagctggtcg
     6721 atgtccgcgg ccaggaccgc gtccaccgtg tcgtaggcat agctctggct gatgagctgg
     6781 ttctgcagac gcccccggaa aaattccaga acatcttttt tgacgtcttc ggccggcttc
     6841 ttcaggacgt ccttcagagc cgccagacat tcgtcgatca gaaagttcag gccgagctcg
     6901 tagcgccgcg acagaatgat attgatgacg ccgagcgcct ggcgacgaag ggcatagggg
     6961 tcggccgtgc cggtgggggg catgcccacg gcgaagaagc cgacaatggt gtccatttta
     7021 tcggcaatgc cgacaatggc gccttcatcc gtccggggca gatcgcctcc ggcgacaatc
     7081 ggcaggtaat gctcgtaaat ggcgtcggcg atttccggtt tctcgccggc cagaagcgcg
     7141 tattcccgtc ccatgatgcc ctggagttcg gaaaattcgc ccaccatgag cgattccaga
     7201 tcggccttgg ccagaagcgc ggcgcggtcc acgtttttct tgacggaggg cttcactttg
     7261 gaagcaattt tgaccgccag cttgcggaag cgcatcacct ttttatggga ggtgcccagc
     7321 agcgtgtgga acacgacttt tttcagggac tccacacggc tctccagcgg gattttcttg
     7381 tcctcctcga aaaagaacga ggcgtcggcc agccgggcct gcagtacgcg ctcattgccc
     7441 cgggcgacca cttcgggatt ccgggcgcgg gtgttgctga cggcgatgaa acagggcaga
     7501 agctttcccg cgttgtccac gactgggaag tacttctggt gcgcgatcat ggtggtggtc
     7561 agcacttcct tggggatctg caggtaattc ggatcgaagc cgccgcgaat gatcaccggg
     7621 tattccacga taaaagtgac ggtttccagc aggtcttccg tatagaaaag ctttcccccg
     7681 gcttcggcgg ccgctttttg cgcttcttcg agaatgaatt cttttctctc cgcgggatcg
     7741 gggatgacaa aatgctttcg ggctttggcg aggtaatctt cgaatccgtc gaccgcaaaa
     7801 gcctccgggc tcatgaagcg atggccgcgc gaggtgttgc cgctttcaat gtcttccacc
     7861 ttcagcggaa cgattttgcc gccgtacaac gccagcagcc agtggatcgg ccgggcaaag
     7921 cgcagatcgt aagccgacca gcgcatggat ttgcgaaacg ggatggacac aagaaagttt
     7981 ttgaggatgt ccggcagcag gtctgcggtc ggctgtccgg caatggtttt tcgcgcgcac
     8041 agatactcgc ccttgtcggt cgtgatggtt tccagttcgg cgatctccag accctgtccc
     8101 cgggcaaacc ccagcgcggc cttggtcggc cggccgtttt cgtcaaaggc cgcctttcgg
     8161 gccggcccca ttttttcgac gatctgatcg gcctgcttcg gcgcgacgtc cgcaatgtgc
     8221 agcaccagcc ggcggggagt ggccaggcag tggacggact cgaaggcgat tcgtttttcg
     8281 gtaagcgcct tgcggatcat ttcttccatg tccacgaccg ctttggataa aaaccccgcg
     8341 gggatttctt cggtgccgat ttcaaaaaga agttcgttgc tcatcgtgtc tccagttttt
     8401 ctagtgattc tgcaaaagag ggtatcccag ttcttcccgc tgcttcatgt agagttccgc
     8461 gctcaccctg gccaggtttc gcacccggcc gatgtagctg gtgcgctccg cgacgctgat
     8521 cgcgccgcgg gcattgagca ggttgaacgt gtgcgagcat ttcaggcagt agtcataggc
     8581 gggaagcgga agatttttct ccgcgatgcg cagcgcctcc tcttcataca tatcaaacag
     8641 cctgcgcagc atcggaatgt cggccacttc aaagttatac gtggaaaatt cgacttcgcc
     8701 cttatggtgc acgtcgccgt atttgatttt atccgtccat tgcaggtcat agacgttgtt
     8761 gatgccctgg atgtacatgg cgatgcgctc cgttccgtag gtcagctcgg cgcagaccgg
     8821 cttgcagtcg aagccgccca cctgctggaa atacgtgaac tgcgagattt ccatgccgtc
     8881 gagccacacc tcccagccca gcccccaggc gcccagcgtc ggggattccc agtcgtcctc
     8941 gacgaaacgg atatcgtgct ccagcggatc gatgccgaag cttttgagcg aattgaggta
     9001 aagctcctgg atgttgaccg gcgacggctt catgatcacc tggtactggt aataatgctg
     9061 caggcggttg ggattttcgc cgtagcgtcc gtcggtcggc cgccgtgaag gctgtacgta
     9121 ggccacgttc cagggttccg ggcccagcgc ccgcaggcag gtggcgggat gaaacgttcc
     9181 cgcgccgacc tccatgtcat aaggctgctg aatgacgcag ccctgggagt gccagtattg
     9241 ctccagcgcg aagattaatt cctgaaaagt cacaatgtcc tccttgggga atgccggcag
     9301 gaactcagga ccggtgatgc gggtccgaga cattacttct gccagcggct acctagcatg
     9361 aaaggccagg ggggtcaaga tacgggaagg gggaagattg gtggatagtg gatagtggat
     9421 ggtggataga aaaacagtaa tgagtgacga acaatgagcg atgagtggaa agtgaaaaga
     9481 tgtcatggtg agcttgtcga accatgggca atgagtcatg gggaataggg catgggtcat
     9541 ttttctgttg acaggccggg gagtcttttc tatgaacaac ttcagtgatt ggaggcgttt
     9601 gtcaaaagcg tttctttact ccggtggagc tgaatatgct caattttacc tttcacaatc
     9661 cgacgaaagt tcttttcggc aaatgcacga tttgtcaact ggcgggtgaa attccgcccg
     9721 gcgccagggt gatggttgcc ttcggcgctg aaagcgccag aaaatacggc gtcctggatg
     9781 aagttctcag ggcgctggcg ggatttgacg tgactgaatt cggcggcatc gaagcgaatc
     9841 cggaatatga tacgctttgc tccggcattg ctctggcccg cgaaaaaaag atcgatttcc
     9901 tcctcgcggt gggcggaggc tccgtcattg acggaaccaa gttcatggcc gccgccattc
     9961 ccttcgccgg cgaaccgtat caactgatgg aaaacaaagg cgcggcgatc cgccaggccc
    10021 tgccgctggg cgcggtcctg acgctgccgg cgagcggttc ggaaatgaac aaccgggcca
    10081 ttatttctcg ccgggctgta aactggaaac gcgccgccat gaacgactgg tttttccctc
    10141 gttttgccgt tctggacccg acaaagacct gcacgcttcc gccgatgcaa accgcgaacg
    10201 gcgtggtcga tacgtttgtc catgttctgg agcagtatct gacttatccc gtgcagggaa
    10261 aggttcagga ccgccttgcc gaagggttgc tgcttctgct gattgaagaa gggccgaaag
    10321 cgctggtgga accggaaaat tacgatgtgc gcgccaacct gatgtgggcc tcgacgctgg
    10381 ggctgaacgg tctgatcggc gccggcgttc ctcaggactg ggcggcgcac cgggcaggct
    10441 atgaattgac ggtgctgtat ggactggacc atgcgcggac gctggccgtg ctggtgccgg
    10501 ccatgctgca ggtccgggcc ggcggaaaaa aggaaaagct gcttcagtat ggcgagcgtg
    10561 tctggggaat atcctccggg gccgaagacg ttcggatcac agccgcgatt gaaaagacaa
    10621 aggctttttt tggaagcatg ggcctgccga cgagcctgtc gggatgcggc atcgaaaaag
    10681 tggatgccga ccggttcatt gaactgcttg aggagcacgg cctcaccgcc atgggcgagc
    10741 ggggcgaggt tactcccgcg atgatgcggg agattctggc gctttgcgcg taaaggaagg
    10801 aatttaagga tgcacagcat tcttcctgaa atcggcattg ccgttctggc ggcgacggcg
    10861 atgggttttc tcttccagct atgccgccag ccggtcattc tgggctatct ggttgccgga
    10921 gcgctgatcg gaccgcagat cggattttcg ctcgtttccc atcccgacaa catcgaagtc
    10981 atctccgaga tcggcctgat ccttctgctg tttatcatcg gcctggagct caatcccgcc
    11041 aaacttcttt cctctggaaa aaaactgatt tatgccggcg tgggccagtt cattttctgc
    11101 gttctgatcg gtttgggatt ttttgtgctg ctggggtatc ccctgggcgg cggacgaatc
    11161 gaagcgcttt acctctcgct gttctgcgct ctttccagca cggccatcgt cgtgaagctg
    11221 ctctacgaca agttcgagct ggacacgctg cccggccgca tcagcctggg cattcttatc
    11281 ttccaggacc tgtgggccat tctgattctg gcgctgcagc cgaatttcat ggatccgaag
    11341 ctgcacctgg tcgcgctggc gctgggcaaa ggcgtcctgc ttctggccgt cggctttttg
    11401 ctcagccgat tcgcgctgcg ctggattttt gccaaaatcg ccaaaactcc ggaaatggtg
    11461 gtcgccacat ccattgcctg gtgcgccttt atggccgggc tgggctcctg gatcggcatg
    11521 tccatggaaa tgggcgccct gatcgccggc gcgg
//
LOCUS       DAOPAF010000049        11401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5587_length_11401_cov_63.469152, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000049 DAOPAF010000000
VERSION     DAOPAF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5587_length_11401_cov_63.469152"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLQ77_05825"
     CDS             <1..189
                     /locus_tag="PLQ77_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10227.1"
                     /translation="SGGLLSGHPVIAAGLYSMAAVVRQIRGDAGGFQVQKAKTGLVQG
                     VNGLGGQSHCVWILDKDK"
     gene            201..1358
                     /locus_tag="PLQ77_05830"
     CDS             201..1358
                     /locus_tag="PLQ77_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10228.1"
                     /translation="MAKRVAIVGTGQTFHKSHRPDVNGQELIHEAVARALADADLKLK
                     DIDAVVIGNMDHFEGINYVDCWSVDGSGGTMKPIIKLTTGGTTGCTVAIGGYHLVGSG
                     MFDRVLIIGWEKNSESDTTGAITTAFDPIWDRLVFAGAISGLAAEAQAYMARYGATDR
                     DGARVSVRDRKHAMNNPHAQLRKEVSLEEVLASPMLADPIHLLDVCPRSDGACAVIMA
                     SEDIAEKICPKPDWIWGTAARHSYSYLGDADYGRLTSMREGSQELWKKAGIKEPRKEL
                     DVVEMYQPYSFGGLIWIEDMGLVGPGEAPRYIWDGNTDMGGELPINPSGGVISCNPIG
                     ATGLIRCAEAAMQVMGKAEGRQVPDVNFAFSSGFGGCWWTDMILHGKKKPA"
     gene            1379..1843
                     /locus_tag="PLQ77_05835"
     CDS             1379..1843
                     /locus_tag="PLQ77_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPL10229.1"
                     /translation="MAEKENIIIESGDAVQPFAYSVGMHGSRFFAEIRDHKRFLGIKC
                     PKCGKVYIPPRGVCGDCFVEMKDWVEVGPKGVIGTFTILRYAFIDPETGAQKPVPYGY
                     GFIRFDGADTLFQHYINIEDEAKIRVGARVEPVFAGERKGSIRDIEYFRVID"
     gene            1843..2745
                     /locus_tag="PLQ77_05840"
     CDS             1843..2745
                     /locus_tag="PLQ77_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10230.1"
                     /translation="MAAKESPTVEINPFKRILKMPGALCGGISTGSDKIRSGYGNGDC
                     LYFDFEHLVFAVADGTERFPWASRDLLQRLSERLLRSGSPETARDWKDMMNTGVYDGQ
                     KYQHKTTFSAVALRREGEDVFLIIANGGDSVVTVMDGLTGKIRRQTGRNMDFAGRSRQ
                     IVDVTEHRMPDRDHRVLLSTDGFDDVWRFYLRRSPAGSARGVLERVGLDGIGEVISGL
                     LEEHRDRFEYDDVGFILLDPGAVKPVKGKALIMGGTRPFEENRYRLQYTPEAHDRWIP
                     DAEWAGQEEMLDGAGIRVLNTVRC"
     gene            2788..3396
                     /locus_tag="PLQ77_05845"
     CDS             2788..3396
                     /locus_tag="PLQ77_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPL10231.1"
                     /translation="MNERNKNDLLNLRRSQLTAAAYKVVAQKGYNDFTIKDIAEEAGL
                     STGLVHYYFKNKEDLLFKLLKEMNANLRDNLKKAMGELAEPRDKLMAFCDEAFALVDR
                     EKAYFYVLIDFWAQINHDARIRQANVKLFQSYRAEIAAIIQEGAAKGVFTVEDVNLTS
                     VIIIALIQGTIIQYAIDREAFNYSEIKEKIKEQILSRIVNGK"
     gene            3416..4567
                     /locus_tag="PLQ77_05850"
     CDS             3416..4567
                     /locus_tag="PLQ77_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL10232.1"
                     /translation="MEFGFTEEQLMFRDSVYKFAKKEIVPLVEEADLKSEFSMEVWRK
                     LGEMGILGLPFPEELGGSGASVETCCLAGEALGHAGVDQGHLLALGAHTYLCADTIFK
                     HGSPEQLKKYVPKLASGEWIGCMGLTEPGAGSDAASITTTAVKKDGKWILNGTKTFIT
                     NAPVCNVCVVYATVDKALKHKGITAFIVERGFPGFATGAPFHKMGVRASATSEVILDN
                     CEVPEENLLGEVGRGFEYTHETLSWDRSSLLAPFVGALQFAIESCTKYSQERVQFGKP
                     INAFQAIQHKLADIKIIQEAAKMVVYRVAHDKDSGKPLNHLHTSIAKSVVGDYGIKAA
                     SDAVQIFGGYGFIHEYPIERFLRDVKLAQIGGGTSEMQKFIISRILSFM"
     gene            4618..5790
                     /locus_tag="PLQ77_05855"
     CDS             4618..5790
                     /locus_tag="PLQ77_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPL10233.1"
                     /translation="MNYDLTPEQLSIKDNFTKFCTKEIEPRAALLDEAPHEEVPGLIR
                     ENIKKMAGIGYLGIGHEEAYGGTNLDLISQAIAGEAVAAACASTFLSCGASSGLFGMP
                     LRLFGSEEQKKKYLPGIIKGEIIGCFGLTEPEAGSDAASIKTAAVKDGDRWILNGTKT
                     FITNATIADVALIFAYNDKAKGPGAGVTCFLVEAGTPGFKAGKPFDKMGFRGSPTAEL
                     ILEDCEVPDSAVLGTVGSGFIQAMMTLEYGRIGMATVCLGIASKCLELANKYSKERVV
                     FGKPINRFQDISFKLADMLIMSDTARLLIYQAAWAKQTGRPESAVLASVAKVWASETG
                     KQISDLAVQIHGGYGYMKEYPVERLYRDARLGEIGEGASEIQRILIAKDLIRRYAA"
     gene            5852..6289
                     /locus_tag="PLQ77_05860"
     CDS             5852..6289
                     /locus_tag="PLQ77_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPL10234.1"
                     /translation="MAIDAKFIGKAYPSYTYEVGREKIKEYAKAIKNFDPHYLEDDFA
                     AKSKYGTIIAPPTFAVVYGAYLIEPVFNDRDLNLNMAMLVHGEQELEFFEVVKAGDVI
                     TTGAKIVHIQNKEKLDIIAIEVQSKNQHGRDVCRGIYTFVVRK"
     gene            6300..6686
                     /locus_tag="PLQ77_05865"
     CDS             6300..6686
                     /locus_tag="PLQ77_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPL10235.1"
                     /translation="MAIEKGQTFSGSEKVDKYRPIYYAGASGDFNPIHIDPEFGKMVG
                     LGGAILQGLCTLAFASKTVTDWAGDPGKLKRLKCRFSAPVLMEDTISVQGTVTDVQSG
                     RARIDLKVVNQNGVEVLQKVEAEVEA"
     gene            6839..8677
                     /locus_tag="PLQ77_05870"
     CDS             6839..8677
                     /locus_tag="PLQ77_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL10236.1"
                     /translation="MASLWVDERDIKFQLFEMFNIGETLLGKGRYADHDADMCNMVLD
                     QAQKFAEVEIAPTYPDEVHRKPVEAVFKDGKVFAPEAYHRLYKLYCEGGWMTTADSPE
                     VGGQAFPFIIGAACANMFLAANQAFLMYPGLTHGAARLVEDFFQNPLRDIILEKMFSG
                     VWAGTMCLTEPGAGSDVGILKTTAKKNADGTYSITGTKCFISAGDHDLAENIIHPVLA
                     RIEGDPAGTKGISIFIVPKIRINEKGELGEPNDVQCGNIESKMGIHGNATCTLNFGTD
                     GKCIGYLMGEERDGMKIMFQMMNEERQGVGMMGVSLAMASYLHALDYAKQRIQGSNII
                     EMAMKVPNPKAVPIIQHPDVRRMLLKQKSITEGIRALAFLCYYSMDREFNAGLEQNEQ
                     DKEYWHGMIEMLTPMVKSYCTERGMEVCSDAVQTYGGYGYCREYPVEQMMRDQKINLI
                     YEGTNGIQAMDLLGRKLGMKKGLYFMNLLGLTQAAVAEAKGNDKLKAEAEIVEGALNA
                     CAGTAMAFAKMMKTTPFVPLIGAADFLNCLSDALVGWLHIWMANAAFKGLANAASDKD
                     KAFYSGKIEGAKFFINRITGLVPAKLENLQKDEQSAMNISEEAFAV"
     gene            8809..10062
                     /locus_tag="PLQ77_05875"
     CDS             8809..10062
                     /locus_tag="PLQ77_05875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPL10237.1"
                     /translation="MMKDVVIVSACRTAIGTFGGSLRDLHVVDLASVAMKEAIKRAGI
                     SPDLIDDVRFGNCFEPYNSLNLTRIAALKAGVPNTSVAVTINRVCISGMEATISGMAM
                     IQAGLADVILAGGAEHMSGIAYAVPGARWGCRLQNSTLDDQLIEGLMCGSRILPAPTD
                     SPLAGGMPYDLYKGKPYIMGHTAEFIAQMYGISREEMDEVALRSHNNVERATKDGDFA
                     EEIVPLEIPQKKGKPPKIFDKDEHFRPGITLEDLRNLPPAFIPKTGKVTAGNASGLND
                     AASATVIMSMEKAKELGLKPLAKIKAVGYGGCDPSVMGLSPVPAVRSLMKRSGLKLED
                     FELIEVNEAFAAQYLGCEKELKINREITNVNGSGIGLGHPVGATGNRIMVTLIHAMKK
                     RKKTLGLATLCGGGGVSIATAIEML"
     gene            10108..10920
                     /locus_tag="PLQ77_05880"
     CDS             10108..10920
                     /locus_tag="PLQ77_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL10238.1"
                     /translation="MKDLRGKIALVTGAAMGMGRSLSEDFLREGCAVALVDVNGPALA
                     EAQAELAKTGRCAAFVCDISNREAVYELARRVEKEFGPVDILVNNAGIVKARELLDLS
                     DDDIEKTIRINLTAQFWTCKAFLPSMIQRGGGHIVNFASAGGILSLPNLSAYCASKFG
                     VVGFSDSLRQEMKKHQYNIGVTYVCPNTVNTGMFEGSKMVAGTRMLSASDVTKKVIRA
                     VKRNQAMVAVPDFPVKILTPLTKVLLPIRAMDWLNKIMGMATCNDSWRGRSC"
     gene            10947..>11401
                     /locus_tag="PLQ77_05885"
     CDS             10947..>11401
                     /locus_tag="PLQ77_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10239.1"
                     /translation="MKALKGILIFAVVLICLSAVVTGINILQKGGAWDGRSMEEILGV
                     APQKATLEDIGKLDKAEIFQLYYAASAPSFHDFRGEFAAVTVDVGILASSAAYFTHHF
                     FGPGRWEGKAFNPSEKDRGYGYNIFRDAGGKVYRTRRMDTEIGPSDYDG"
BASE COUNT         2637 a         3101 c         3487 g         2176 t
ORIGIN      
        1 tccggcggac tgctttccgg gcatcccgtc atcgccgccg gcctctacag catggccgcg
       61 gtggtccggc agattcgggg agacgcgggc ggttttcagg tgcaaaaagc gaaaaccggc
      121 ctggtgcagg gtgtcaacgg gctgggcggc cagtcccact gtgtgtggat tctggacaag
      181 gacaagtagg aggacaaacc atggcgaaac gagttgcaat tgtaggaacc ggacagacct
      241 tccataagag ccatcgcccc gacgtcaacg gacaggagct gatccatgag gcagttgccc
      301 gtgcgctggc ggacgccgac ctgaagctga aggacatcga cgccgtcgtc atcgggaaca
      361 tggatcattt tgaggggatc aactacgtgg actgctggag cgtggacggt tccggcggga
      421 cgatgaagcc cattatcaag ctgaccaccg gcggaaccac cggctgcacc gtcgccatcg
      481 gcggatatca tctggtcggc tcgggaatgt tcgaccgagt gctgatcatc ggctgggaaa
      541 agaactccga atcggacacg accggcgcca tcaccacggc ttttgatccc atctgggacc
      601 gcctggtctt cgccggcgca atatcgggtc tggcggcgga ggcgcaggct tacatggccc
      661 gctacggcgc gacggaccgc gacggcgccc gggtgagcgt gcgcgaccgg aaacatgcga
      721 tgaacaatcc gcacgcgcag cttcgaaagg aagtgagcct ggaagaagtg ctggcttccc
      781 cgatgctggc ggacccgatc catcttttgg acgtttgccc ccgctccgac ggcgcgtgcg
      841 cggtcatcat ggccagtgaa gacatcgccg aaaagatctg cccgaaaccg gactggatct
      901 ggggcacggc tgcgcgccac tcctacagct acctgggcga cgccgattac ggccgcctga
      961 ccagcatgcg cgaaggatcg caggaactct ggaagaaggc gggcatcaaa gagccgcgca
     1021 aagaactgga tgttgtcgag atgtaccagc cctattcgtt cggcggtttg atctggatcg
     1081 aggacatggg cctggtgggc cccggcgagg cgccgcggta tatctgggac ggcaacaccg
     1141 acatgggcgg cgaactgccg atcaatccgt cgggcggcgt gatttcctgc aatcccatcg
     1201 gggcgaccgg gctgatccgc tgcgcggaag cggccatgca ggtgatgggc aaagccgaag
     1261 gccggcaggt tccggacgtc aactttgcct tcagtagcgg tttcggcggc tgctggtgga
     1321 ccgatatgat tctgcacgga aagaaaaaac cggcctgaag caacaaggag gaaccagcat
     1381 ggcggaaaaa gaaaacatca ttatcgaatc gggcgacgcg gttcagccct tcgcgtattc
     1441 cgtgggcatg cacggaagca ggttttttgc ggagattcgc gaccataagc ggtttctggg
     1501 gatcaagtgc cccaaatgcg ggaaggtgta tattccgccg cgcggcgtct gcggagactg
     1561 ctttgtcgag atgaaagact gggtcgaagt cgggccgaaa ggggtcatcg gcacctttac
     1621 gattcttcgc tacgcgttta tcgacccgga aacgggcgcg cagaagccgg tcccctatgg
     1681 ctacgggttt atcaggtttg acggcgccga caccctgttc cagcactaca tcaacattga
     1741 ggatgaagcg aagattcgcg tgggcgcccg cgtggagccg gtatttgccg gagagcgaaa
     1801 gggcagcatt cgggatattg agtattttcg ggtcatcgac tgatggccgc aaaggagtcg
     1861 ccgacggttg agatcaatcc attcaagcgg attctgaaga tgccgggggc tctgtgcggg
     1921 ggaatcagca ccggcagcga taaaatccgc agcggatacg gcaacgggga ctgcctttac
     1981 tttgattttg agcacctggt cttcgccgtg gcggacggca cggaacgctt cccctgggcg
     2041 tcgagggatc ttttgcagcg gctgtcggag aggcttttgc ggtccggttc cccggaaacg
     2101 gcccgggatt ggaaggacat gatgaacacc ggggtctatg acggccagaa gtaccagcat
     2161 aagacgacct tcagcgccgt cgcccttcgc cgtgaaggag aggatgtttt tctgatcatc
     2221 gcgaacggag gcgacagcgt cgtaacggtc atggatggcc ttacgggaaa gattcgccgt
     2281 cagacggggc gcaacatgga ttttgcggga agaagcaggc agattgtgga tgtgacggag
     2341 caccggatgc cggaccggga ccaccgggtg cttctttcca cggacgggtt cgatgatgtc
     2401 tggcgcttct atctgcgccg gtcgcccgcc ggctccgcgc gcggcgttct cgaaagggtt
     2461 gggctggacg gaatcggcga ggtgatttcc gggcttctgg aagagcaccg ggaccgtttt
     2521 gagtatgatg atgtcggctt tattcttctg gatccgggcg cggtgaagcc ggtgaaggga
     2581 aaagctctga tcatgggagg aacgcggcct tttgaagaaa atcgctaccg gctgcagtat
     2641 acaccggagg cgcatgaccg gtggatcccc gatgccgagt gggccggaca ggaggaaatg
     2701 cttgacggcg cgggaatccg ggtgctgaac acggtgcggt gctgaaggac cggattccct
     2761 ttcatgcaag aaagtgttgc agaagagatg aatgagcgga acaaaaatga tttgctgaat
     2821 ctgaggcggt cccaactgac ggcggcggcg tacaaagtgg ttgcgcagaa ggggtacaac
     2881 gatttcacca tcaaggatat cgccgaggaa gccggcctgt ccacgggcct ggtccactac
     2941 tacttcaaaa acaaggaaga tcttcttttc aagctgctca aggagatgaa cgccaatctt
     3001 cgggacaatc tgaaaaaggc catgggggag cttgccgaac ccagggataa actgatggct
     3061 ttctgcgacg aagcctttgc gctggtggac cgggaaaagg cgtattttta cgtgctcatc
     3121 gatttttggg cgcagatcaa tcacgatgcc aggattcgcc aggcgaacgt caaacttttt
     3181 cagagctacc gcgccgagat cgcggcgatc attcaggaag gggcggccaa aggcgttttc
     3241 accgtggagg acgtgaacct gacgtccgtg atcatcatcg cgctcattca gggaaccatc
     3301 atccagtacg cgattgatcg cgaggcgttc aattacagcg agatcaagga gaaaatcaag
     3361 gagcagattc tctccagaat cgtcaacgga aaataatgca ttaaaggagg cattcatgga
     3421 attcggtttt acggaagaac agctgatgtt cagagattcg gtctataagt tcgcaaaaaa
     3481 ggaaattgtg ccgctggtcg aggaggcaga tctcaaaagc gagttctcca tggaggtgtg
     3541 gcggaaactc ggtgagatgg gcatcctggg ccttccgttt cccgaagaac tgggcggatc
     3601 gggcgccagt gtagagacct gctgcctggc cggcgaagcc ctgggccatg ccggcgtcga
     3661 tcagggacat ctgcttgccc tgggcgccca cacttacctg tgcgcggaca cgatcttcaa
     3721 gcacggctcg cccgaacagc tgaaaaagta cgttccgaag ctggccagcg gcgaatggat
     3781 cgggtgcatg ggactgacgg agccgggcgc cggttcggac gccgcgtcca tcacgaccac
     3841 ggcggtcaaa aaagacggca aatggattct caacgggacg aagactttca ttaccaacgc
     3901 gccggtctgc aacgtctgtg tggtgtatgc cacggtggac aaagcgctca aacacaaggg
     3961 catcaccgcc ttcatcgtcg agaggggctt ccccggattt gcaaccggcg cgcccttcca
     4021 caaaatgggc gtgcgggcat ccgcgacctc cgaagtcatt ctggacaact gcgaagttcc
     4081 ggaagaaaac ctgctgggcg aggtcggccg gggatttgaa tacacccatg agaccctgtc
     4141 ctgggaccgc agctcccttc tggcgccttt cgtcggcgcc ctgcagttcg ccatcgagtc
     4201 ctgcaccaaa tactcccagg aaagggtgca gttcggcaag ccgatcaacg cgttccaggc
     4261 gattcagcac aagctggcgg acatcaagat catccaggag gccgccaaaa tggttgttta
     4321 ccgcgtggct cacgacaagg attccggaaa gcctttgaat catttgcata cgtccatcgc
     4381 gaaatccgtg gtcggcgact acggcatcaa agccgccagc gacgcagtcc agattttcgg
     4441 cggctacggg ttcatccacg aatatccgat cgagcggttt ttgagggatg tgaaactggc
     4501 gcagatcggc ggagggacat cggaaatgca gaaattcatc atctcccgca tcctgagctt
     4561 tatgtagcct caggccggaa gcagaataac cagacatcca ttgaaggaga atatgccatg
     4621 aactatgatt taacgccgga acagttgtcg ataaaggaca attttacgaa attttgcaca
     4681 aaggaaatcg aaccgcgcgc cgcgctgctc gatgaagcgc cccatgaaga agtccccggg
     4741 ctgatcagag aaaatatcaa gaagatggcc ggcatcggct atctcggcat cggacacgaa
     4801 gaagcttacg gcggaacgaa tctcgacctg atcagccagg ccatcgcggg ggaagcggtc
     4861 gcggccgcct gcgcgtccac ctttttatcc tgcggcgcat cctccggcct gttcggcatg
     4921 ccgctgaggc tgttcggctc cgaagagcag aagaaaaaat atcttcccgg catcatcaaa
     4981 ggggaaatca tcggctgttt tggcctcacc gaaccggaag ccggctccga cgccgcgtcc
     5041 atcaagacgg ccgccgtgaa agacggggac cgctggattc tcaacggaac caaaacattc
     5101 attaccaacg ccaccattgc cgacgtggcg ctgatcttcg cttataacga caaggcgaag
     5161 ggaccggggg ccggcgtgac ctgcttcctg gtggaggcgg gcacaccggg gttcaaagcg
     5221 ggcaaacctt tcgacaaaat gggatttcgc ggctccccga ccgccgaact gatcctcgag
     5281 gactgcgaag tgccggacag cgccgttctg ggcacggtgg gcagcggctt tatccaggcc
     5341 atgatgacgc tggaatacgg ccggatcggc atggcgaccg tttgcctggg cattgcgtcc
     5401 aagtgcctgg agctggccaa caagtattcc aaggagcgcg tggtcttcgg aaagccgatc
     5461 aaccgctttc aggacatttc gttcaagctt gccgatatgc tgatcatgtc cgatacggcg
     5521 aggctcctca tttatcaggc cgcgtgggcc aagcagaccg gccgtcccga gtccgccgtg
     5581 ctggcctccg tggccaaagt ctgggcttcc gagaccggca agcagatctc cgacctggcc
     5641 gttcagattc acgggggcta cggctacatg aaggaatacc ccgtggagcg cctctaccgc
     5701 gacgcgaggc tgggagaaat cggcgagggc gcttcggaaa tccagcgcat cctgatcgcc
     5761 aaagacctga tccggaggta cgcggcctag ccgttcagac gaaggcgcgc gctttccgac
     5821 cggagacatg acgctgcaag gagaaagacc catggccatc gacgcaaaat tcatcggcaa
     5881 ggcctaccca tcctatacct atgaggtggg cagggaaaaa atcaaagagt atgcgaaagc
     5941 gatcaagaat ttcgatccgc attatctgga agacgatttc gccgccaaat cgaagtacgg
     6001 aacgatcatc gccccgccca cctttgcggt ggtctatggc gcctacctga tcgagccggt
     6061 gttcaacgac cgggatctga acctgaacat ggcgatgctg gtccacggag agcaggaact
     6121 ggagtttttc gaggtggtca aggcgggaga tgtcatcacc accggcgcaa agatagtgca
     6181 cattcagaac aaggaaaagc tggatatcat cgccattgaa gttcagtcga aaaaccagca
     6241 cggccgggac gtctgccgtg gaatttacac ctttgtcgtc agaaagtagg aggcacgcca
     6301 tggccattga aaaaggacag accttcagcg gaagcgagaa ggtcgataaa tacaggccca
     6361 tttactacgc cggcgcatcc ggcgatttca atccgattca tatcgatccc gaattcggga
     6421 aaatggtcgg gcttggaggg gcgattcttc agggattgtg cacacttgcc tttgcgtcca
     6481 aaaccgtcac cgactgggcg ggagatcccg ggaagctcaa gaggctcaaa tgccggttca
     6541 gcgctccggt gctgatggaa gatacgattt cggtccaggg aaccgtcacg gatgtgcagt
     6601 cgggacgcgc caggattgat ctgaaggtgg tcaaccagaa cggcgtggaa gtgcttcaga
     6661 aggttgaagc cgaggtcgaa gcgtaaaatg cagccgtttg cagaataaat attcccgaac
     6721 gcttgacaat tgttctcaat tgccatatga acttcgtcga agcaccttcc cgcacgctct
     6781 tcagggggaa accggaacga ttactcgttc ataaataaaa gaaaacaagg aggtttgtat
     6841 ggcaagtttg tgggttgacg aaagggatat caaattccag ctttttgaaa tgttcaacat
     6901 cggggaaacg ctcctgggaa agggccgtta cgccgatcac gatgcggata tgtgcaacat
     6961 ggtgctggac caggcgcaga aatttgccga ggtcgagatc gcgcccacct atccggatga
     7021 ggtccaccgc aaacccgtcg aagcggtctt caaggacggc aaggttttcg caccggaagc
     7081 ctatcaccgt ctctacaagc tttattgcga aggcggctgg atgacgacgg cggattcgcc
     7141 cgaagtcggc ggtcaggctt ttccgttcat catcggggcg gcctgcgcca acatgtttct
     7201 ggccgccaat caggcgtttc tcatgtaccc cggtctgacc cacggcgcgg cgcgcctggt
     7261 ggaggacttt ttccagaatc ccctgcggga cattattctt gaaaagatgt tctccggtgt
     7321 ctgggccgga acgatgtgcc tgaccgagcc gggcgccggc tccgacgtgg gcattctgaa
     7381 aactacggcc aagaagaacg ccgacggcac gtattccatc accggcacca aatgtttcat
     7441 ctccgccggc gaccatgatc tcgccgaaaa catcattcac ccggtcctgg cccgcatcga
     7501 aggcgatccc gccggcacca agggcatctc cattttcatc gttcccaaga tccgcatcaa
     7561 cgagaagggc gagctgggcg aacccaacga cgtgcagtgc ggcaacatcg aaagcaaaat
     7621 gggcatccac ggcaacgcca cctgtacgct gaacttcggc accgacggca agtgcatcgg
     7681 ctacctcatg ggcgaggagc gcgacggcat gaagatcatg ttccagatga tgaatgaaga
     7741 gcgccagggc gtgggcatga tgggcgtatc gctcgcgatg gcctcttacc tgcacgcgct
     7801 ggattacgcc aagcagcgca ttcagggctc gaacatcatt gaaatggcca tgaaggttcc
     7861 gaatccgaaa gcggttccga tcattcagca tccggacgtg cgccgcatgc tcctcaagca
     7921 gaagagcatc acggaaggga ttcgcgcgct ggctttcctg tgctactact ccatggacag
     7981 ggaattcaac gccggcctgg aacaaaacga gcaggacaaa gaatactggc acggcatgat
     8041 cgaaatgctg accccgatgg tcaagtccta ctgcaccgag cggggcatgg aagtatgctc
     8101 ggatgccgtg cagacctacg gcggctacgg ctactgccgg gagtacccgg tcgaacagat
     8161 gatgcgcgat cagaagatca acctgattta tgaaggcacc aacggcattc aggccatgga
     8221 cctgctgggc cgcaagctcg gcatgaagaa gggcctgtat ttcatgaatc ttctgggact
     8281 gacccaggcc gccgtcgccg aagccaaagg caatgacaaa ctcaaggccg aggccgaaat
     8341 cgtcgaaggc gcgctcaatg cctgcgccgg aaccgccatg gcctttgcca agatgatgaa
     8401 gacgacgcct tttgttccgc tcatcggcgc ggccgacttc ctcaactgcc tgtccgacgc
     8461 cctggtgggc tggctgcaca tctggatggc caacgccgcc ttcaaaggcc tggccaatgc
     8521 cgccagcgac aaggacaagg cgttttattc cggcaagatc gagggcgcaa aatttttcat
     8581 caaccgcatc accggtttgg tcccggccaa gctggaaaac ctgcagaagg acgagcagag
     8641 cgcgatgaac atcagcgaag aagcctttgc cgtttaaccg cattgtaaga agacccaatc
     8701 cgaggaggaa ccggaaccgt aacggttcct cctttttttg tcgagcgtcg tttggcggac
     8761 agccaatgaa aaatcatttc aattcataac aaaaacaggg agaacaagat gatgaaggat
     8821 gtggttattg tttcagcctg ccggacggcg atcggcacgt tcggcggatc gttaagagat
     8881 ctgcatgtgg tggatctggc gagcgttgca atgaaagagg ccatcaagcg ggccggcatc
     8941 agtccggacc tgattgacga cgtccgcttt ggaaattgtt ttgagcctta caattcgctg
     9001 aacctcaccc ggatcgcggc cctgaaggcg ggggttccga atacgtcggt tgccgttacg
     9061 atcaaccggg tttgcatctc gggcatggaa gccacgattt ccgggatggc catgatccag
     9121 gcgggtctgg cggatgtgat tttggccggg ggcgccgaac acatgtcggg cattgcctat
     9181 gcggttcccg gcgcgcgctg gggctgccgg ctgcagaaca gcacgctgga cgatcagctc
     9241 attgaaggtc tgatgtgcgg atccagaatc ctccccgctc ccacggacag ccctctggcc
     9301 ggagggatgc cctacgacct ttacaaaggc aagccctata tcatggggca caccgcggaa
     9361 tttatcgccc agatgtacgg catttcccgc gaagaaatgg acgaagtcgc gctgcgcagc
     9421 cacaataacg tggagcgcgc gacgaaggat ggggatttcg ccgaagaaat cgttccgctc
     9481 gaaattccgc agaaaaaagg caagccgccc aagatttttg acaaggatga acacttccgg
     9541 ccgggcatta ccctcgaaga cctgcgcaat cttccgccgg ccttcattcc caaaaccggc
     9601 aaggttacgg cgggcaacgc ttcgggtctg aacgacgcgg ccagcgccac cgtcatcatg
     9661 tccatggaga aagcgaagga gctgggcctc aagcccctgg ccaagatcaa ggcggtcgga
     9721 tacggcggat gcgacccgtc cgtcatggga ctgagccccg tccccgcggt tcgaagcctg
     9781 atgaagcgct ccggcctgaa gctggaggat tttgagctga tcgaggtgaa cgaagcgttc
     9841 gccgcccagt acctgggatg tgaaaaagag ctgaaaatca accgggagat tacgaacgtg
     9901 aacggctccg gaatcggcct gggccatccg gtcggcgcaa ccggcaaccg gatcatggta
     9961 acgctcatcc acgcgatgaa aaagcgcaaa aagacgctgg gcctcgccac gctgtgcggc
    10021 gggggaggcg tttccatcgc aacggccatt gaaatgctgt aagcggtccg gcggcaaaca
    10081 ccggcccgtc atcggggagg gaagaccatg aaggatttgc ggggtaaaat tgccctggtt
    10141 acgggcgcgg cgatgggcat gggccggagt ttgtcggagg atttcctcag ggaaggatgc
    10201 gcggtcgcgc tggtggacgt gaacggaccg gcgcttgcgg aggcccaagc cgagcttgcg
    10261 aaaacaggca ggtgcgccgc cttcgtctgc gatatttcca acagggaggc ggtttacgag
    10321 ctggcccgcc gggtggaaaa ggaattcggt ccggtcgata ttctggtcaa caacgccggc
    10381 atcgtcaagg cccgggagct gctcgatctt tccgacgacg acattgaaaa aaccatccgc
    10441 atcaatctca ccgcccagtt ctggacgtgc aaggcgtttc tgccctccat gattcaaagg
    10501 ggaggcggcc atatcgtcaa cttcgcctcg gcgggcggca ttctgtctct tcccaatctt
    10561 tccgcttact gcgcaagcaa attcggcgtg gtgggcttct ccgattcact gcgccaggaa
    10621 atgaaaaaac atcagtacaa catcggggtc acctacgtat gccccaacac ggtgaatacc
    10681 ggcatgtttg aaggatcaaa gatggttgcg ggcaccagaa tgctttccgc ctccgacgtg
    10741 accaaaaagg tgatccgggc cgtcaagcgc aatcaggcca tggtcgcggt gccggatttt
    10801 cccgtgaaaa tcctgacgcc gctcaccaag gttcttcttc ccatcagggc gatggactgg
    10861 ttgaacaaaa tcatgggcat ggccacctgc aacgattcgt ggagaggccg gagctgttaa
    10921 aaaataaatt tcaggacggg aataagatga aagcattaaa gggaattctg atttttgccg
    10981 ttgttttgat ttgtctgtcg gccgtggtga ccggaatcaa tattcttcag aagggcggcg
    11041 cctgggacgg aagaagcatg gaggagattc tgggcgttgc gcctcaaaag gcgaccctgg
    11101 aggacattgg caagctcgac aaggcggaga tcttccagct ttactatgcc gcttccgcgc
    11161 cttcctttca cgatttccgg ggcgaatttg ccgcggtgac ggtggatgtc ggcattctgg
    11221 cgtccagcgc ggcttatttc acccatcact ttttcggtcc gggacgctgg gagggcaagg
    11281 ccttcaaccc gtctgaaaaa gacaggggat acgggtacaa tattttcagg gatgccgggg
    11341 gcaaagttta ccggacgcgc agaatggaca cggaaatcgg accgtcggat tacgacggca
    11401 a
//
LOCUS       DAOPAF010000050        10896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5879_length_10896_cov_90.493589, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000050 DAOPAF010000000
VERSION     DAOPAF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_10896_cov_90.493589"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLQ77_05890"
     CDS             <1..548
                     /locus_tag="PLQ77_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPL10240.1"
                     /translation="IGGGTTEVAVISLSGIVYAKSVRMAGDKIDDEIVQYMKRKYSLL
                     IGERTAELIKTEVGCAYPMAEIKTIDVKGRDLISGIPKTIEVSSDEIREAIIEPVTMI
                     VDTIKDALENAPPELAGDIVDRGIVLTGGGALLKNLDILVREETGLPVTVADDPLSAV
                     ARGAGMALDQLDVLKEVTIQV"
     gene            599..1444
                     /gene="mreC"
                     /locus_tag="PLQ77_05895"
     CDS             599..1444
                     /gene="mreC"
                     /locus_tag="PLQ77_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPL10241.1"
                     /translation="MFFKNYRAVLFVGAVILAALFLFSYGLKYDVGQSLVQRLALGAA
                     APVQKLFTLCVESVGNAWTRYIRLVGLEEENRKLKTEIGELEARMTLYRQGHLEAQRL
                     QALLSLAEREPYRFIAARVIGREQAALAKTLWIDKGEAQGLKPGMPVMALPGLIGRLT
                     NVSLHSARVQLLIDEGSSADVLVERTRQQGMLRGAGSRGCVIRYISKIQDVREGDVIL
                     TSGLSNIFPKGLLVGKVVHTDRADAGMFLNIRVAPFADFTSLEEVLVLAGEKKDPFRE
                     RKAAK"
     gene            1441..1929
                     /locus_tag="PLQ77_05900"
     CDS             1441..1929
                     /locus_tag="PLQ77_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10242.1"
                     /translation="MMYYILLPFMAVVLVVLQSKLAEILFSGLVTLELSLIVVIYAGF
                     RLELWKGAALSLVVGLAMDCLSGSLLGLFTLLYVFIFLLAWFVSSQIDSQRLYLIAGF
                     SLVCSAAESLALVLLYQWVFGFDMVDKILPVLAPQTLLVSVLSVCFFYAMRRVERLLA
                     YG"
     gene            1922..3826
                     /gene="mrdA"
                     /locus_tag="PLQ77_05905"
     CDS             1922..3826
                     /gene="mrdA"
                     /locus_tag="PLQ77_05905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPL10243.1"
                     /translation="MDKPGRLQNGPEPVDFRRKLAVVMILVCAALSVLMIRLWYLQIV
                     KGDELKQRSENNAVRFRRIQPLRGLIMDRNGFVIVDNRPSFDVLYLPARGVDHEALLA
                     KLQDLYAVKSLEFPEGQTLPRTLKSYLPVRLEKNVGMEKVALVETNSLDLPGVYVDVT
                     PVRLYLDGEVIAPIIGYTGEVSREELEKPDGDYASGDITGKSGIEKALDSYVRGRRGN
                     ELVEVNVTGKVIKNLGRIDPVPGSNVVLTIDAALQKSAWEAFEGRPGAAVAMDPQTGA
                     ILAMVSSPSFDPNLFNSGIAREEWEKLQKDPLKPMSNRAIAGQYPPGSTYKLIVAAAA
                     LEEGVITPETRIHCNGSFVLGNRTYRCWRKNGHGPVNLHRALVESCDVYFYTVGKKLG
                     VDRIADYAKRFGLGDATGVELAHERKGLVPTKSWKLARVKEPWQMGETISISIGQGFN
                     LTTPLQLAQAYAALANGGTLWRPHLVQRIEAPQGGVVREYPPEKKGEIGLDGKIAALL
                     RQALWGVVNENGGTGYAARIAGADVCGKTGTSQVVGLPQDEQARRRKKIAAFHKDHAL
                     FVCYAPARRPEIVVSVVAEHAGGGGAVAAPIARRILKAYFDGKKNERPRQAAAGSQTP
                     PPSGNGKPQG"
     gene            3842..4954
                     /gene="rodA"
                     /locus_tag="PLQ77_05910"
     CDS             3842..4954
                     /gene="rodA"
                     /locus_tag="PLQ77_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPL10244.1"
                     /translation="MFFKYDRRIFQNFDKTLFALVMSICAIGVVNIYSTGFSLGENAT
                     PLYLKQIQWIAFGLFFMMVAFLIDYRYFNRAAYGLYAFSMASLVAVALFGHTANGAQR
                     WISFGVFLFQPSELMKISIILALARYFDDHKSREPYRLRELLIPFGMVMIPCALILKQ
                     PDLGTALMIVIVSASIVLFIGMNWKSVAIAVASIFVMLPLAWHFLKDYQKNRLLTFLS
                     PENDPLGTGYHIIQSMIAIGSGGFLGKGFLKGYQTQLKFLPEQQTDFVFSVFAEEWGF
                     LGGLALMVIFTSLIIWGLKIALHSKDLLGTIIAFGITALIAWQVLINMGMVLGVLPVV
                     GIPLPFISYGGSAVVSLMAAIGLLMNVSVRRFILQR"
     gene            4986..5363
                     /locus_tag="PLQ77_05915"
     CDS             4986..5363
                     /locus_tag="PLQ77_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPL10245.1"
                     /translation="MWEKKKRLDDTKAFLGKGAEFIGKLIFTGHVRIDGDFQGEIFGQ
                     GAVDIGEGALVKANMEVGSVFIGGEVQGNIDVKEKINIHSTGKLAGDVKAPVFIMEEG
                     AVFDGQSRMSEVKEQKRIALAGK"
     gene            5654..6088
                     /locus_tag="PLQ77_05920"
     CDS             5654..6088
                     /locus_tag="PLQ77_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPL10246.1"
                     /translation="MVTVIPDYTLLVQMATFIALIFILNFLLYKPLLSIIERRKKQLD
                     ELGNEIKLFNESVNKKAAEYEEKLSRAKTSASDLKKQIIGEGAAEAKRIVDAVRSEIP
                     LMTQEFQKKMDAEMQAARQILEGQSRRLSLEIAEKVLGRRVQ"
     gene            6085..6678
                     /locus_tag="PLQ77_05925"
     CDS             6085..6678
                     /locus_tag="PLQ77_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPL10247.1"
                     /translation="MKSSFRSVHFSILLSLVLLLLSVGLVFASGGEGGEGHGSQWKDF
                     AWKTFNAVLIIGFLVYLLGARIKGFFSGRRQEIKESLESATVQKAEAEKQYREYAEKI
                     DKASQEIDGIFEMIKAQGVVEKQQIIEDAHKVAQKMKEDAQARIEQELKKASGQLRAE
                     AVALSVQMAEEILKKNITAQDHEVMVREYMDKVVNKN"
     gene            6675..7217
                     /gene="atpH"
                     /locus_tag="PLQ77_05930"
     CDS             6675..7217
                     /gene="atpH"
                     /locus_tag="PLQ77_05930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPL10248.1"
                     /translation="MISNISKRYARAFFDIAGEEQKLEQYYNELHQFSSVIAQNKDLG
                     GFLANPIFEQEIKKKVLEKIIGKLSLSPMTINFLKLLIDKKRIDILPDIETCYRQLMD
                     ETLQKVRVTVKTAFPLSGEMQSYITSSLKKMTGRTVDVTVENDKNLLGGIVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            7221..8735
                     /gene="atpA"
                     /locus_tag="PLQ77_05935"
     CDS             7221..8735
                     /gene="atpA"
                     /locus_tag="PLQ77_05935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPL10249.1"
                     /translation="METIKAEEISQIISEQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTHIKEGDIVKRTGKIAQVPVGE
                     GLLGRVIDATGAPLDGKGPIESTEFRRIEMIAPGVIQRQPVNQPMYTGLKAIDAMTPI
                     GRGQRELVIGDRQIGKTAICVDAIIRQKDTGIKCIYVAIGQKKSTVAQVVERLKQHDA
                     LSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDRGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAYPGDIFYNHSRLLERAARVSKELGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFFSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFAQFGSDLDKSTQAQLDRGVRLVELLKQPQFQPMSLGEEVVVLFAGSRGFIDKVD
                     VEKVLVYEQQLLSYVKSKHQDILKEIDEKQIISADLEKKMKDMLTGFESVFVAE"
     gene            8813..9691
                     /gene="atpG"
                     /locus_tag="PLQ77_05940"
     CDS             8813..9691
                     /gene="atpG"
                     /locus_tag="PLQ77_05940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPL10250.1"
                     /translation="MPSLKDIKRKVTAVQKTKQITRAMNMVAASKFKSAQLKMENFRP
                     YAGKFMDVLNSLALRVDTSTHPLLAVRDPRRIRVICMTSDRGLCGGFNTNLIKATERF
                     MRDKTRDGKEVSMISVGRKGRDFFRKKANVLAQKVDCLSKFDMTLAVSVADEVIDPFI
                     KAEYDELYLIYNQFVNVSVQRPTVVRLFPLPSIGSPDADMETDTRLDYVYEPSDEVLL
                     HKLLPMYVHVLVYRALLETSAGENGARMAAMDNATSNCEELIHTLTLKMNKARQAAIT
                     AELMDIVGGTEALAKG"
     gene            9753..>10896
                     /gene="atpD"
                     /locus_tag="PLQ77_05945"
     CDS             9753..>10896
                     /gene="atpD"
                     /locus_tag="PLQ77_05945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPL10251.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGRLPSILNALLITNSAINDQEDNL
                     VIEVAQHLGDNVVRCIAMDITDGLVRGMIAKDTGAPITVPVGKEGLGRILNVVGRPVD
                     GLGPINAKNYASIHRSSPPFLEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMINNIAMHHGGISVFCGVGERTREGNDLYREMLESGVIKQAALIYGQMTEP
                     PGARSRVALTGLAAAEYFRDVEGQDVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATDLGELQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNVIGQEHYTVARQVQVTLQKYKDLQDII"
BASE COUNT         2413 a         2860 c         3267 g         2356 t
ORIGIN      
        1 acatcggcgg cggcaccacg gaagtggcgg tcatctcgct gtccggcatc gtgtatgcca
       61 agtcggtgcg catggccggc gacaagatcg atgatgaaat tgttcagtac atgaagcgga
      121 aatacagtct gctcatcggc gagcggacgg cggaattgat caagacggaa gtcggctgcg
      181 cctatccgat ggcggagatc aaaacgatcg acgtgaaggg gcgcgatctc atctccggca
      241 ttcccaagac gatcgaagtc agctccgatg aaatccggga agcgatcatc gagccggtga
      301 cgatgatcgt ggacacaatc aaggatgcgc tggaaaacgc gccgccggag ctggccggag
      361 acatcgtgga ccgcggcatc gtgctgaccg gcggcggagc gcttttgaag aatctcgata
      421 tcctggtgcg ggaggaaacc ggtcttcccg tcacggtggc cgacgatccg ctcagcgccg
      481 tggctcgcgg cgccggcatg gcgctggatc agctcgatgt gctcaaagag gtgacgattc
      541 aggtctgacg ccgcctggcc gcccggtctt ccaaggcggc ggatagtttg ggtagatcat
      601 gttttttaaa aattatcgag ccgtcctctt tgtcggagcg gtgattctcg ccgcgctttt
      661 ccttttttcg tacggcctga aatacgatgt cggccagagc ttggtgcaga ggcttgcgct
      721 gggggcggcg gcgccggttc aaaaactgtt cacgctgtgc gtcgaaagcg tcggaaacgc
      781 ctggacgcgc tacatccgcc tggtgggtct ggaagaagaa aaccggaaac tgaagacgga
      841 aatcggcgag ctcgaggcgc ggatgacgct ctaccggcag ggacatctgg aggcccagcg
      901 gctgcaggcg cttctgtcgc tcgcggagcg ggagccctac cgctttatcg cggcccgcgt
      961 gatcggcagg gagcaggcag ctctggccaa gaccctctgg atcgacaagg gagaagcgca
     1021 ggggctgaag ccgggcatgc cggtgatggc gcttcccgga ctcatcgggc ggctgacgaa
     1081 tgtttccctg cacagcgcca gggttcagct tctgattgac gagggctcca gcgcggatgt
     1141 gctcgtcgaa aggacgcgtc agcagggaat gcttcgcgga gcgggatcgc gcgggtgcgt
     1201 catccggtac atctccaaga ttcaggacgt tcgggaaggg gatgtgattc tgacgtccgg
     1261 cctgagcaac atttttccca aggggctgct ggtcggaaag gtggtccata cggaccgggc
     1321 ggacgccgga atgtttctga atatccgggt ggctcctttt gccgatttta cctcgctgga
     1381 ggaggtgctg gttctggcgg gggagaaaaa ggatccgttc cgggaaagga aggccgcaaa
     1441 atgatgtatt acattctgct gcctttcatg gccgtcgtgc tggtggttct gcagtccaag
     1501 ctggccgaaa ttcttttttc cggcctcgtc acgctggagt tgtcgctgat cgtggtgatc
     1561 tacgccggct ttcgcctgga gctgtggaag ggagcggccc tttcccttgt cgtggggctg
     1621 gcgatggatt gcctgtccgg gtcccttctc gggctgttca cgctgctgta tgtcttcatt
     1681 ttcctgctcg cctggtttgt ttcctcgcag attgattcgc aaaggcttta cctgatcgcc
     1741 ggcttcagcc tggtctgttc ggcggcggaa tcgctggcgc tggttctgct ctaccagtgg
     1801 gtgtttggct tcgacatggt cgataaaatc ctccccgtcc tggcgccgca gaccctgctg
     1861 gtcagcgttc tgtcggtgtg cttcttttac gcgatgcgca gggtggaaag attgttggcg
     1921 tatggataaa ccgggcagac ttcaaaacgg gccggagccg gtggactttc ggcgcaagct
     1981 tgccgtggtg atgatcctgg tttgcgcggc cctgtccgtc ctgatgatcc gcctgtggta
     2041 tctgcagatc gtcaaggggg acgagctcaa gcagcgctcg gaaaacaacg ccgtccgctt
     2101 tcgcaggata cagcccctgc gcggcctgat catggaccgc aacggttttg tgatcgtgga
     2161 caaccgcccc tcctttgacg tgctgtacct gcccgcacgg ggggtcgatc acgaggcgct
     2221 cctcgcgaag cttcaggacc tctacgccgt caaatccctt gaatttcccg agggccagac
     2281 tcttcccaga acgctgaagt cctatcttcc cgtccggctg gagaaaaacg tcggcatgga
     2341 aaaagtggcc ctggtggaaa ccaacagcct ggatctgccg ggggtctatg tggatgtcac
     2401 gcccgtccgg ctttacctgg acggggaggt catcgcgccg atcatcggct acacggggga
     2461 agtcagcagg gaggagctgg aaaagccgga cggggattac gcctcgggcg acatcacggg
     2521 gaaatcgggc attgaaaaag cgctggattc ctatgtgcgc gggcgccggg gcaatgaact
     2581 ggtcgaagtg aacgtgaccg gcaaggtcat caagaatttg gggcggatcg atccggttcc
     2641 cggctccaac gtggtgctga cgattgacgc cgcccttcag aagtcggctt gggaggcctt
     2701 tgagggaaga ccgggcgcgg cggtcgcgat ggatccgcaa accggcgcca ttctggcgat
     2761 ggtcagctcg ccgtctttcg acccgaattt attcaacagc ggcattgccc gtgaagagtg
     2821 ggaaaagctg cagaaggatc cgctgaaacc catgtccaac cgggcgattg ccgggcaata
     2881 tccgcccggc tcgacctaca agctgatcgt ggcggcggcg gcgctggaag agggggtgat
     2941 cacccccgaa acccggatcc actgcaacgg atcttttgtt ctgggcaacc ggacctatcg
     3001 ctgctggaga aaaaacgggc acgggccggt caatctgcac cgggcgctgg tggagtcttg
     3061 cgacgtctat ttttacaccg tcgggaaaaa gctgggcgtg gatcgcatcg cggactacgc
     3121 gaaacggttc ggcctgggcg acgcgaccgg cgttgagctg gcccatgagc gaaagggtct
     3181 ggttcccacg aagagctgga agctggcccg ggtgaaagag ccgtggcaga tgggcgaaac
     3241 catatcgatt tccatcggac agggattcaa tctgaccact ccgctgcagc ttgcgcaggc
     3301 ttacgccgcg ctggccaacg gcggcacgct gtggcgccct cacttggtgc agcggattga
     3361 agcgccccag ggcggcgtgg tccgggaata tccgcctgaa aaaaagggag agatcggcct
     3421 cgacggaaaa atcgcggcgc tcctgaggca ggcgctgtgg ggcgtcgtga atgaaaacgg
     3481 gggcaccggc tacgcggcgc gcatcgccgg cgcggacgtc tgcggcaaaa cggggacttc
     3541 gcaggtggtc ggtctgccgc aggatgaaca ggcgcgccgc cggaaaaaaa tcgcggcgtt
     3601 tcataaggac catgcgctgt ttgtctgcta cgcgccggcc aggcgtccgg aaatcgtagt
     3661 atccgtcgtt gccgagcacg ccggcggcgg cggcgcggtg gccgcgccga tcgcccgcag
     3721 gattttaaag gcgtatttcg acggcaagaa aaatgagcgg ccccggcagg cggctgcagg
     3781 ttcgcaaacg ccgccgcctt ccggaaacgg aaagccgcaa gggtaagcac ggggaggctt
     3841 tatgtttttc aaatacgatc ggcgcatctt tcagaatttc gacaagacgc tgtttgccct
     3901 ggtgatgtcg atctgcgcca tcggcgtcgt caatatctat tccaccggct tcagcctggg
     3961 cgaaaacgcg acacccctgt atttaaaaca gattcaatgg attgccttcg gcctgttttt
     4021 catgatggtc gcctttttaa tcgattaccg ctacttcaac cgcgcggcgt acggccttta
     4081 cgcgttttcg atggcgtcgc ttgtcgcggt cgcgctgttc ggccacacgg ccaacggggc
     4141 gcagcggtgg atctcgttcg gcgttttttt attccagcct tccgagctga tgaagatcag
     4201 catcatcctc gcgctggccc ggtattttga tgatcacaag tcgcgcgaac cttaccggtt
     4261 gagggaattg ctgattccgt tcgggatggt gatgattccc tgcgccctga ttttgaagca
     4321 gccggatttg gggacggcgc tgatgatcgt cattgtttcc gcatccattg ttctttttat
     4381 cggaatgaac tggaaatccg tcgcgattgc cgtcgcgagt attttcgtca tgcttcccct
     4441 ggcctggcat tttttaaagg attatcagaa aaaccgcctg ctgacgtttc tttcccccga
     4501 gaatgacccg ctgggcacgg ggtatcacat cattcagtcc atgatcgcaa tcggctccgg
     4561 gggatttctg ggcaagggct tcctgaaggg ctatcagacc cagctcaagt ttctgccgga
     4621 acagcagacc gattttgtgt tttccgtgtt tgccgaggag tgggggtttc tgggcggcct
     4681 ggcgctgatg gtgattttta catccctgat catctggggg ttaaaaattg ccttgcattc
     4741 aaaagattta ttgggcacga tcatcgcgtt cggcattacc gcgctgatcg cctggcaggt
     4801 gctgattaat atgggaatgg tgctgggcgt cctgccggtg gtcggcattc cgctgccttt
     4861 catcagttac ggcggctcgg cggttgtttc tctgatggcg gcgatcggac tgctgatgaa
     4921 tgtgagcgtg cgcagattca tcctgcagcg ctgattttat tttcaggacg actaaggagt
     4981 gcggcatgtg ggaaaaaaag aaacgtcttg acgacaccaa agcttttttg gggaagggcg
     5041 ccgaatttat cgggaagctg atttttaccg gccatgtgcg cattgacggc gattttcagg
     5101 gggaaatttt cggacaggga gcggtggata tcggagaagg cgccctggta aaggccaaca
     5161 tggaagtcgg cagcgttttc atcggcggcg aagttcaggg caatatcgac gtgaaggaaa
     5221 aaatcaacat tcattccacg gggaaacttg caggcgatgt gaaggcgccg gttttcatta
     5281 tggaggaggg agcggttttt gacggccaat cgcggatgag cgaggtgaaa gagcaaaaaa
     5341 gaatcgcttt ggctggaaaa taataattcc caagtgataa attttattag cttaaatccg
     5401 atttttgcga aattagaccg ccggtcataa aaaagcttga caaaccctag gcgggtgtga
     5461 ttagtaacct ccgtcctcgc ggtgtggcta ggttcagcat aagcccggct tgattcaagc
     5521 cttacgttat ctgaagcaaa tcactcccag agattacgct gtttgaaatg tcggatcaac
     5581 tttaatgcat cttcctgcgt tgatcaacgt tcgttgatgg ccgggaaggt ctgaaaaaaa
     5641 gacgaggtca gttgtggtta ccgtaattcc cgattatacg ctcctggttc agatggctac
     5701 gttcatagcc ttgattttca tcctcaattt tctcctctac aaaccccttt tgtccatcat
     5761 cgagcgccgc aagaagcagc tggatgaact gggcaacgag atcaagcttt tcaatgagtc
     5821 cgtcaataaa aaggccgccg aatacgagga aaagctgagc cgggccaaga ccagcgcttc
     5881 ggacctgaaa aagcaaatca tcggcgaagg cgccgcggag gcgaaaagaa tcgtggacgc
     5941 cgttcgcagt gagattcccc tcatgaccca ggagtttcaa aagaagatgg acgcggaaat
     6001 gcaggccgcc cgtcagatcc ttgaaggtca gtcccgccgg ctctcgctgg aaattgccga
     6061 aaaggttctg ggaaggagag ttcaatgaaa tcttcatttc gttcggtcca tttttcaatt
     6121 cttctctccc tggttttgct gctgctttcc gtggggctgg tttttgcgtc cggcggcgaa
     6181 ggcggcgagg ggcacggctc gcagtggaaa gactttgcct ggaaaacctt caatgccgtt
     6241 ctgatcatcg ggttcctcgt gtatttgctt ggggcgagaa tcaaaggttt cttttccggc
     6301 cgccgccagg aaatcaagga atctctggag agcgcaaccg ttcaaaaggc ggaagccgaa
     6361 aaacagtacc gggagtatgc cgagaaaatc gacaaggcct cccaggagat tgacggcatc
     6421 tttgagatga tcaaggcgca gggagtggtc gaaaagcagc aaatcatcga ggacgcgcac
     6481 aaggtggccc agaagatgaa ggaggacgcc caggcgcgca ttgagcagga attgaaaaaa
     6541 gcctccgggc agcttcgcgc ggaagcggtc gccctttccg tgcaaatggc ggaagaaatt
     6601 ttaaagaaga acatcacagc tcaggatcat gaagtcatgg tcagggaata tatggataag
     6661 gtggtgaaca aaaattgatc agcaatatat caaaacgata cgcccgcgct ttttttgaca
     6721 ttgccggaga agaacagaag ctggagcagt actacaacga actgcatcag ttctcttccg
     6781 tcatcgcgca aaacaaggat ctgggaggtt ttctggccaa tccgatcttt gaacaggaaa
     6841 tcaagaaaaa agtgctggag aaaatcatcg gcaagctgtc gctttcaccc atgacgatta
     6901 attttttaaa attgctgata gacaagaaaa ggatcgacat cctcccggac atcgagacct
     6961 gctaccggca gttgatggat gaaacgcttc agaaagtgag agttacggtg aagacggcgt
     7021 ttccgttgtc cggggaaatg cagagctaca ttacgtccag cctgaaaaaa atgacgggcc
     7081 ggaccgttga cgtgacggtg gagaacgaca agaatctgtt gggcggcata gttattggtg
     7141 ttggtgatac gttgtatgac ggcagcatca aaaaccaatt gaacaatatg aggaatctct
     7201 taggggaggc aagatagaac atggaaacca tcaaggcgga agaaatcagt caaattattt
     7261 ctgagcagat caaaagctat gaaaaaaagc tggatataag cgaaaccgga accgtccttt
     7321 ccgtcggtga cggcatcgcg agagtgtacg gcgtgcaaaa cgctatggcc atggaacttc
     7381 ttgagttccc cggcggtatt ctgggcatgg tgctcaacct cgaagcggac aacgtcggcg
     7441 tcgcggttct gggagaagtg acccatatta aggaagggga tatcgtcaaa agaaccggaa
     7501 agattgccca ggttccggtc ggagaggggc ttctgggccg cgtcatcgac gcgacaggcg
     7561 ctcccctgga cggcaaagga ccgatcgaat caacggagtt ccgccgtatt gaaatgatcg
     7621 cccccggcgt gattcagcgc cagccggtca accagcccat gtacacgggc ctcaaagcca
     7681 tcgatgccat gaccccgatc ggacgcggcc agcgcgaact ggtcatcggc gaccgccaga
     7741 tcggcaaaac cgccatctgc gtggatgcca tcatccgcca gaaagacacc ggcatcaaat
     7801 gcatctacgt cgcgatcggc cagaaaaaat ccaccgtggc gcaggttgtg gaaaggctca
     7861 aacagcatga cgccctgtcc tatacctgtg tggtcgccgg ctgcgcctcc gacccggcaa
     7921 cccttcagta catcgcggct tacgccggct gcagcatcgg cgaatacttc cgtgaccgcg
     7981 gccaggacgc cctcattatt tacgacgacc tgtccaaaca ggccgtggcc tatcgtcaga
     8041 tttcgttgct gcttcgccgt ccgcccggac gcgaagctta ccccggcgac attttctaca
     8101 accactcccg tctgctcgag cgggccgccc gcgtgagcaa ggagctgggc ggcggatcgc
     8161 tcacggctct gcccatcatt gaaacgcagg ccggcgacgt atcggcctac attccgacca
     8221 acgtgatttc catcaccgac ggccaggtgt acctggaacc cagtttgttc ttttccggca
     8281 tccgtccggc catcaacgtc ggtctgtccg tgtcccgcgt cggcggcgcg gcccaggtga
     8341 aggcgatgaa acaggtcgcc ggcaccctga agctcgatct ggcccagtac cgcgaactgg
     8401 cggcctttgc ccagttcggc tccgatctgg acaaatcgac gcaggcccag ctcgaccgcg
     8461 gcgttcgtct ggtcgaattg ctgaagcaac cccagttcca gccgatgtct ttaggcgagg
     8521 aagtggtggt tctgtttgcc ggaagccgcg gcttcatcga caaggtggac gtggaaaagg
     8581 ttctggtcta tgagcagcag ctgctgagct acgtaaagag caaacatcag gatatcctga
     8641 aagaaatcga tgaaaagcag attatttccg cggacctgga aaagaaaatg aaagatatgc
     8701 tgacgggttt cgaatctgtt ttcgtagcgg aataaattgt aatccatgcg cggtcctcaa
     8761 aaagaggatc tccatggagc ggaaaaccaa ttaatgttga gaggagtttc cagtgccctc
     8821 gctaaaagat attaaaagaa aagtaacggc cgttcagaaa acgaagcaga ttacgcgcgc
     8881 catgaacatg gtggctgcat ccaagtttaa atccgctcaa ctgaaaatgg aaaatttccg
     8941 gccctatgcc ggaaaattca tggatgttct caacagtctg gcccttcggg tggacacctc
     9001 cacgcacccg cttctggccg tgcgcgatcc cagaagaatc cgggtgatct gcatgacgtc
     9061 cgaccgggga ctctgcggag gcttcaatac gaatctgatc aaggcaacgg aacggtttat
     9121 gcgggacaag acccgtgacg gcaaggaagt ttccatgatc agcgtcggcc ggaagggcag
     9181 ggattttttc cgcaaaaagg caaacgtcct ggcgcaaaaa gtggactgtt taagcaagtt
     9241 tgacatgacc ctcgcggtgt cggtcgcgga tgaagtgatt gatcccttta tcaaggcgga
     9301 gtacgatgaa ttgtatttga tttataatca gttcgtgaat gtttccgttc agaggccgac
     9361 cgttgtccgc ctgttcccgc tgccctccat cggcagcccg gacgccgata tggaaacgga
     9421 tacgcggctg gattatgttt acgaaccgtc ggacgaggtg ctcttgcaca aattgctgcc
     9481 gatgtatgtt catgttctgg tgtatcgggc tctgctggaa acgtccgccg gcgaaaacgg
     9541 cgcgcggatg gcggcgatgg acaacgccac cagcaactgc gaagagctga tccacacgct
     9601 gacgttgaaa atgaacaaag cgcggcaggc ggcgattacg gcggaattga tggatatcgt
     9661 aggcggtacg gaagctttgg cgaaaggttg attttgcggc ggcgttttgc gcgtaaaaaa
     9721 tataaattac gactattggc aaggagatag ttatgaatat cggaaagatt gttcaggtta
     9781 ttggtccggt tgttgacgta gcttttgaag aaggaaggct tcccagcatt ttgaatgcgc
     9841 ttttgattac caactccgcg atcaacgatc aggaagataa cctggttatt gaagtcgcgc
     9901 agcatctggg tgacaacgtt gtccgctgca tcgccatgga tatcacggac ggtctggtcc
     9961 gcggcatgat tgccaaagat accggcgctc ccattaccgt gccggtcggc aaagaaggcc
    10021 tcggcagaat tctgaacgtg gtcggccgtc ccgtggatgg tctcggaccg atcaacgcca
    10081 aaaattacgc ttccatccat cggtcgtctc cgcccttttt ggaacaggat acatccgttc
    10141 acgttctgga aaccggcgtt aaggttatag atctcttggt tccgtttccc cgcggtggca
    10201 aaatgggcat gttcggcggc gccggcgtcg gcaaaaccgt cgtcatgatg gaaatgatca
    10261 acaacatcgc catgcaccac ggcggcatct ccgtgttctg cggcgtggga gagagaaccc
    10321 gcgaaggaaa cgatctgtat cgcgaaatgc tggaatccgg cgttatcaag caggccgccc
    10381 tgatttacgg ccagatgacc gaaccccccg gagcccgctc ccgcgtggcc ctgaccgggt
    10441 tggcggccgc cgaatacttc cgtgatgtgg aaggtcagga cgtgctcttc ttcgtcgata
    10501 atattttccg tttcacccag gccggctccg aagtgtccgc tctcctgggc cgcatgcctt
    10561 ccgccgtcgg ttatcagccg acactggcca cggatcttgg cgaacttcag gaacgcatta
    10621 cctcgaccac caaaggttcg attacagccg ttcagtgcgt gtacgtgccc gccgacgact
    10681 tgaccgaccc cgcgccggcc acgacgtttg cgcaccttga cggaaccgtc gttttgtccc
    10741 gtccgatcgc cgaactcggc atttaccccg ccgtggatcc gctggattcc acctcccgaa
    10801 ttctcgatcc gaacgttatc ggtcaggaac attacaccgt cgcccgccag gtgcaggtga
    10861 cgctgcagaa gtataaagac ctgcaggaca tcattg
//
LOCUS       DAOPAF010000051        10805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_5943_length_10805_cov_43.838791, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000051 DAOPAF010000000
VERSION     DAOPAF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_10805_cov_43.838791"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLQ77_05950"
     CDS             complement(<1..676)
                     /locus_tag="PLQ77_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPL10252.1"
                     /translation="MPQEDLSKLKIDSSAKQSVAGGRKRKPFVIAGALTLLAAAVILY
                     RLGVIAPSATVDVTTVSMIYPAQSLSVLNASGYIVAQRKAAVASKVTGRLTQLLVEEG
                     SRVRKGQILARMESGDVAAMKEQGTANLANAQAMLKQTLVERDHAAQENERYQKLIAG
                     GYVAQSDYDMIHTRYLRSLEAVKAAQAAVESAGAALSHAEVNLDYTLIRAPFDGVVLT
                     KNADVGD"
     gene            complement(683..1078)
                     /locus_tag="PLQ77_05955"
     CDS             complement(683..1078)
                     /locus_tag="PLQ77_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10253.1"
                     /translation="MPVLEQHLPLFILIMAKAQCKGNEDKEKKKGRSEPEDIQAAGQQ
                     GLTRGLLCFFRCHKAPCVPFDQAGKMADPFVSGCKETVSEPINFGRIMKTTPSNVKRQ
                     KRSKNGFLCVWQIQPGSVILPANSFQLLR"
     gene            1058..2977
                     /locus_tag="PLQ77_05960"
     CDS             1058..2977
                     /locus_tag="PLQ77_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPL10254.1"
                     /translation="MLLKDRQSAQLLSLIAETYIHDIRTTIEAYASRPLLTQAVREKD
                     ASEARRHLLMLTRMNPYIDSVVIADKKGTVWAALPDRPDILGKNFSFRDWYRGVSREW
                     KPYIAEAIIRSTGEKDTAIHIAVPIFNDKKEVLGILLSPLRAVEMGKVLRRAPMEEGL
                     FIAVTDRKGTLLYSSRFAHEKKLVRYPFFDAVKTKPLTTVTVRDPAAQEEERHISYAP
                     VAEAGLHVFVGRDQSSVRSKFWGHLAQISSISILLFVLFVLLLLYARKNVIAQSALES
                     LKAEKEISAGRSRFSELFNQLKSGVAIYQAIDGGRDFEILELNDAARRITKVGPNAEG
                     GSVLAVYPGLANHTILATFQNVWRTGNPEEHPTFLYQDDRQTFWADNYVFKLPSGEIV
                     SVFDDVTARKLAADKISRQTRLLSAINRLFAETLKSQDTEAVARTCLAAALSITDSEF
                     GFIGEVGPDGLFSTYTVSETGWDACSMPQKEATASLQNMIIRGIWGQVILREQPLIVD
                     DPGAFPDRVGVPEGHPPLTSFLGVPLKDGRKTIGMIALANRTGGYTGEQREDLEELAV
                     SLVEALRRNQAEEKVKNMYQELEKRVIERTGELVAKTEELERINRVFVDRELKMRELK
                     ARIAELEKKATSDER"
     gene            2986..4845
                     /locus_tag="PLQ77_05965"
     CDS             2986..4845
                     /locus_tag="PLQ77_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10255.1"
                     /translation="MVYLDLIFNLSLLVALSVVSGFLDKRFSRDTRTGALLQGALFGA
                     VAIVGMLRPLTLGPGLIFDGRSVMLSLCALFFGPPAAAVSCVMALACRISIGGAGLIM
                     GILTSLSSTAIGLIFFYQNKSSLQPPSAKLLYVMGLAVHAAVAALMFTLPESLFWSTF
                     QNLAAPILLLFPLATLLAGKILSDHLSMMQSASALSESEERCKSLFEASLDAVMLTAP
                     DGRILSANPAACRLLDRSEEELIDLGRGGIMDREDPRLAGALAERERTGKFSGELTMI
                     RKDETKFPVEITTAVFHDRFGQARTSMIFRDITRRLREEEHLKKLQALLAETQRLSKI
                     GGWERDLTTGRGQWTEEVYNIYGISPDAQPSTQEAMDFYAPEDRPVITEAFGRLLETG
                     KPYDLELRFNKATGETIRVRTIGQAEWRDGKIVRVFGNIMDVTERVRAQEALRESEEL
                     FRNLFMHHTAVKLILDPETGDIIDANKAAAEFYGWPRERLLQMKIFDINTLTEEEIRT
                     GLRKVKNEKRTRFQFRHRRADGSIRDVEVFSSRIDVRGKPFLHSIITDITERKEMENR
                     MRQMNEELEQKVQERTAELKKTINQLEETNKVFVGRELKMAELKKRIEELETK"
     gene            4889..7102
                     /locus_tag="PLQ77_05970"
     CDS             4889..7102
                     /locus_tag="PLQ77_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10256.1"
                     /translation="MILDTRTIVLSYIATDIACLAVMVLLWRQNRERISGTHLWVIYY
                     IVSLFALILMILRGAIPDGASVILVNALFMAGTLIGYQALLRFAGEIRRQTFNYFILA
                     AAILIHAYFTFGKPDVNARIFNLSAVLLVFFSQCAWFCLRGAPEAQRKLMWWPGLVFV
                     LHILVSLCRMAAAVFAPHAQADLFQQNTTIALSVIAYQLLFVALTFALALMYNQRLVE
                     EVRANEEKFELAFQSAPYTLVLSRLSDGKILAINDSFTRITGYSEADIRGKTAADLKL
                     WVNEEDRAIVVRDLRETGAVFERKLLFRKKSGEIMTGLLSAKWITIQDEKCLLFSVND
                     VTEREKVLEELRTAQEQWQKTFDSTQDAIWLMDRNHNILRSNKAAQRLFDRPAENMTD
                     RHCWEVVHGTSAPTPECPILLARESLRRESMDLSIAGKWYLVTVDPILDKEERFAGAV
                     HTMTDISERRQAQEEINHLNETLEQKIADQTRDLRDSQAALLNLVDDLNENAKNLTMI
                     NDSLEEVNRELASFSYSVSHDLRAPLRTIDGFSNALMEDYSDRLDAEGNKYLERIHHA
                     TQQMNRLIDDLLGLSRVMKSDFYRQDFNLSEVVREICADFHQQPSEGRPDLVIQDGIA
                     VNADQRMMRIVMTNLLDNARKFAGKKERPILEFGSFVENGETVFFVRDNGVGFNMTYA
                     QRVFEPFERLHRADEFPGTGIGLATVARVISRHRGRIWAEAQEGKGATFYFTLGA"
     gene            7105..8496
                     /locus_tag="PLQ77_05975"
     CDS             7105..8496
                     /locus_tag="PLQ77_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10257.1"
                     /translation="MPKPLNVLMIEDAEDDELLLTRALKKNGYEPKVERVQTAREMTR
                     ALENSPWDIILCDYHMPAFSGIEAIDLLKNTGLDIPLIMVSGAIGEETALDCIHRGAS
                     DYIMKGNLTRLGMAVKRGLEEKAFRDHHRQDEEALRRSEEKYRTILESIGDGYYETDL
                     AGSFTFFNDALQRIWGYPKEELLGLNNRQYMDGETAKMLYQAHNRTYKTGIPGKLMDY
                     EIIRKDGGRIAIQQSFTLLKDSLGNTIGFRGVVRDITHLKALEQEREETLGKLRQTLE
                     STVHAMAVAVESRDPYTAGHQRRVTELAYAIAATMGLEPNRINGLRMASMIHDLGKIS
                     IPTEILTKPTKLTKIEFEIVKTHAEAGYEILKDIAFPWPIARIVWEHHEKIDGSGYPR
                     HLKGDDLLTESKILTVADVVEAMASHRPYRPSLGLQDALEEITRNRGILYDADAVAAC
                     LTLFEEKRFSFAI"
     gene            8614..10779
                     /locus_tag="PLQ77_05980"
     CDS             8614..10779
                     /locus_tag="PLQ77_05980"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HPL10258.1"
                     /translation="MASPKSGTTTSPWTDWQKQLNEYLVDSAQRSIIFADILRKRGNN
                     YIKHIRGGQPPVLTFNYEMILNAKHFERPVNYALVRISDRRRKDIDPEQQNRRRALRE
                     RPDIPKRPIVIIDPRAGHGPGIGGSKRDSEIGMALDHGHPVYFVLFYTDPLPGQTLPD
                     VIAAIERFIEKVTQLHPDADAPAVIGNCQAGWAAALACADKPGIAGPLALNGAPLSYW
                     SGVEGVNPMRYRGGLLGGVWVNSFFSDLGNGVFDGANLVLNMELLNPANTYWTKQYNV
                     YSKADTEEERYLNFEKWWGGFFMMNDDEIHFIVRNLFVGNKLERGELELREGRKVSLK
                     DIESPILVFASMGDNITPAQQALNWIPRVYSSVEDIKRHGQVIVYIVHQEIGHLGIFV
                     AGSIAKKEHHEIIGNFDMIDCLPPGLYEMVIDGDVGSGKFESRFEPRTMADIAAYDDG
                     IHDETDFSVVAKVSEANDNFYQLLVRPFVKLWTTELSAEFIRHLHPLRVQRYSLADIN
                     PLTRPLQNVANVVRRNRKAAGPDNPFTELEKAVSDYVEGVLDIYRDYRDLYQEEIFQQ
                     IYGNGWLRSLFPPVQDEPKPLAAKQDHPDYDKRLAAMKQGGFAEGLIRIYMAAGGTNQ
                     GIKRKHFEVASEIAATHRVLSKLSPSQFKKIMREQAAILQADEERALQALAALIKNRD
                     DRMEALSIARRLFLADGVYNDEEKVLLEKIRKGLGLEAV"
BASE COUNT         2481 a         3133 c         3063 g         2128 t
ORIGIN      
        1 tgtcgcccac gtcggcgttc ttggtcagca cgacgccgtc aaacggcgcc cggatgagcg
       61 tgtagtccag gttgacctcc gcgtggctga gcgcggcgcc ggctgattcc acggcggcct
      121 gagcggcttt caccgcttcc agcgaccgca gataccgggt atgaatcatg tcgtaatccg
      181 actgagccac atagccgccg gcgatcagct tttgatagcg ttcattctcc tgcgcggcat
      241 gatcgcgctc caccagcgtc tgcttgagca tggcctgagc attggccagg ttggccgtcc
      301 cctgttcctt catggcggcg acgtcgccgc tctccatgcg ggccagaatc tgacctttcc
      361 ggacgcggct gccctcttcc accagcaatt gggtcaggcg gccggtcacc ttggacgcca
      421 cggccgcctt gcgctgggcc acgatgtagc cgctggcatt caagacggac agggactggg
      481 cgggataaat catcgaaacg gtggtcacat ccaccgtggc cgaaggcgcg atcacgccca
      541 gacgatagag aatgacggcg gccgcaagca aagtcagcgc gccggcaatg acaaacggct
      601 ttcttttacg accgcccgcc acgctttgct tggccgatga atcgattttc agcttgctca
      661 gatcttcctg cggcatattt attcacctta acaactggaa agaatttgcc ggaagaataa
      721 cagaaccggg ctgaatttgc catacacata aaaaaccatt tttactcctt ttttgcctct
      781 tgacattgga gggcgttgtt ttcataatgc ggccaaaatt gattggttca gaaacagttt
      841 ctttacaccc ggacacaaaa ggatcggcca ttttgccggc ctgatcaaag ggaacacaag
      901 gcgccttatg acaccggaaa aagcagagaa gcccccgagt cagcccctgc tggccagccg
      961 cttgaatatc ttcgggctcg cttcggccct ttttcttctc cttgtcttca tttcctttgc
     1021 actgagcttt cgccattatc aggatgaaaa gcggcagatg ctgctcaagg accggcaatc
     1081 cgcccaactg ctgtccctga ttgccgaaac ctatatccac gatattcgaa ccaccattga
     1141 agcctatgcc agccgtcctc tcctgacgca ggccgtccgg gaaaaagatg cctctgaagc
     1201 aagacgccac ctcctcatgc tgacccgaat gaatccttat atcgacagcg tcgtcatcgc
     1261 ggacaaaaaa ggaacggttt gggccgccct gccggaccga ccggacattt tgggaaaaaa
     1321 cttttcgttt cgcgactggt accggggcgt cagccgggaa tggaaaccct atatcgcgga
     1381 ggcaatcatc cggtccacag gcgaaaaaga cacggccatt catatcgccg tcccgatttt
     1441 taatgacaaa aaggaagttc tcggcatcct gctttccccc ctgcgggcgg ttgagatggg
     1501 caaagtcctt cggcgcgcgc cgatggaaga gggactgttt attgccgtca cggaccggaa
     1561 aggcacgctt ctatacagca gccggtttgc ccatgaaaaa aagctggtcc ggtacccgtt
     1621 ttttgacgcg gtcaaaacca aacccctgac caccgtcacg gtcagagatc cggccgcaca
     1681 ggaagaggag cgccacattt cgtacgcgcc ggtcgcggag gctggcctgc atgtttttgt
     1741 gggccgggat cagagttccg tccggtccaa attctggggc cacctcgccc agatctcctc
     1801 catttccatc ctgctgtttg tgctctttgt tctgcttttg ctctatgccc gcaagaacgt
     1861 catcgcccag tccgcactgg agagcctgaa agcggaaaaa gaaatttcgg ccggccggtc
     1921 gcggttcagc gaacttttca accaattgaa aagcggcgta gccatctatc aggccatcga
     1981 cgggggaagg gattttgaaa ttctggaact caacgacgca gcccgccgaa tcaccaaggt
     2041 gggcccaaac gcagagggcg gaagcgtcct ggccgtctat ccgggactgg cgaatcacac
     2101 gatcctggcc acttttcaaa acgtctggcg gacgggaaat cccgaagagc accccacctt
     2161 tctttatcag gacgaccggc agaccttttg ggcggacaat tacgttttca aacttccttc
     2221 cggcgagatc gtttccgttt tcgacgacgt gaccgcgcgc aaactcgcgg cggataaaat
     2281 ttcaagacag acccgcctgc tttccgccat caaccggctt tttgccgaga cgctgaaatc
     2341 ccaggacact gaagcggtcg caaggacctg ccttgccgcc gccctgagca ttacggacag
     2401 cgagttcgga tttatcggtg aagtgggccc ggacggactt ttttccacat acaccgtcag
     2461 cgaaacgggc tgggacgcct gctccatgcc ccagaaggag gcaacggctt ctctgcaaaa
     2521 catgatcatt cggggcatct ggggacaggt gatccttcgg gagcagcctc taattgtcga
     2581 cgatccggga gctttccccg accgcgtggg cgttccggag gggcatccgc cgctgacatc
     2641 ttttctgggc gtgccgctca aagacgggag aaaaaccatc gggatgattg cgctcgccaa
     2701 ccgcaccggc ggctacacgg gcgagcagag agaagacctg gaggagctgg cggtttccct
     2761 ggtggaggcc ctgcggcgca accaggcaga agaaaaagtc aaaaacatgt atcaggagct
     2821 ggaaaaacgc gtcatcgagc gcaccgggga gctggtcgcc aaaaccgaag aactggagcg
     2881 gatcaaccgc gtctttgtgg accgcgagct caaaatgcgg gaactcaagg cccggatcgc
     2941 ggagttggaa aagaaagcga cgagcgatga gcgatgaggt tttaaatggt ttacctggat
     3001 ttaatattca atctgtcgct tctggtggcg ctcagcgttg tttcggggtt tctcgataag
     3061 cgcttttcgc gcgatacccg gacgggggcg ctcctgcagg gggcgctgtt cggcgccgtg
     3121 gccatcgtcg gcatgctccg cccgcttacc cttggaccgg ggctgatctt cgacggccgc
     3181 tcggtcatgc tcagcctctg cgctttattt ttcggtcctc cggctgccgc cgtctcgtgc
     3241 gtcatggcgc tggcctgccg catcagcatc ggcggcgcgg ggctgatcat gggaattctg
     3301 acatcccttt cttccaccgc cataggactg atcttttttt accaaaacaa atcctcgctg
     3361 caaccgcctt ccgcgaagct tctctacgtg atgggactcg ccgtccacgc ggccgtggcg
     3421 gctttgatgt ttacgcttcc ggaaagtctt ttttggagca ctttccaaaa ccttgccgct
     3481 cccatcctcc tcctttttcc cctggccaca ctgctggcgg gaaaaattct ttccgaccat
     3541 ctttcaatga tgcagtccgc ctcggctctt tcggaaagcg aagagcgctg caagtctttg
     3601 ttcgaagcaa gcctggacgc ggtcatgctg acggcccccg acggccgaat cctttccgcc
     3661 aatcccgccg cctgccgcct gctggaccgc agtgaagagg aactgatcga cttgggccgc
     3721 ggcggcatca tggaccggga agatccccgg cttgccgggg cgcttgccga gcgcgaaagg
     3781 acaggaaaat tttccggcga actgacgatg atccgcaagg acgaaaccaa atttcccgtg
     3841 gaaatcacga ccgccgtttt tcatgaccgc ttcgggcagg cgcgcaccag catgatcttc
     3901 cgcgacatca cccggcgcct gcgggaggag gaacacctga aaaaactgca ggcgcttctg
     3961 gcggaaaccc agcggctgag caaaatcggc ggctgggaac gtgatctgac aacgggccgg
     4021 ggccagtgga ccgaggaagt ctataacatt tacggcatct ctccggacgc tcagcccagc
     4081 acacaagaag cgatggattt ttacgctccg gaagaccgcc cggtcatcac cgaggccttt
     4141 gggcgactgc tggaaacggg caaaccctac gacctggaac tccggtttaa caaggcaacg
     4201 ggagaaacca ttcgtgtgcg caccatcgga caggcggaat ggagggatgg aaaaatcgtc
     4261 cgggtcttcg gcaacatcat ggacgtcacc gaacgcgtgc gcgcgcagga ggcgcttcgc
     4321 gaaagcgaag aacttttcag aaacctgttc atgcaccata cggcagtcaa attgattctt
     4381 gatccggaga cgggggacat catcgacgcc aacaaagccg ccgcggaatt ttacggctgg
     4441 ccccgcgagc ggcttttaca aatgaaaatt tttgacatca acacgctgac ggaggaggaa
     4501 atccgcacgg ggctgcgcaa ggtcaagaat gagaaacgca cacgatttca attccgccac
     4561 cggcgggccg acggctccat tcgcgacgtc gaggttttca gcagccggat cgacgttcgg
     4621 ggaaagccgt ttctgcattc gatcatcacc gacatcaccg aacgaaaaga aatggaaaac
     4681 cgaatgcggc aaatgaacga ggagctcgaa caaaaggttc aggagcgaac ggccgagctg
     4741 aaaaaaacca ttaaccaact tgaagaaaca aacaaggtat ttgtcggccg ggagctgaaa
     4801 atggccgagc tcaaaaaacg gatcgaagag ctggaaacaa aataaaacac ggcgatgacc
     4861 ggtcgtcatc ccgcagccga ggcctgtaat gattctggac acccgcacca ttgttttatc
     4921 ctatatcgca acggacatcg cctgcctggc cgtcatggtt ctgctctggc ggcaaaaccg
     4981 cgagcgaatc agcggaacgc atctgtgggt catctattat atagtaagtc tgtttgccct
     5041 catcctgatg attctgcggg gcgctattcc cgacggggca tcggttatcc tggtcaacgc
     5101 cctgtttatg gccggcacgc tgatcggtta tcaggcgctt ctgcgctttg ccggtgaaat
     5161 tcgcagacaa actttcaatt atttcattct ggcggcggcg attttgatcc acgcctactt
     5221 cacgttcggc aaacccgacg tgaacgcccg aatcttcaat ctgtcggcgg tgctcctggt
     5281 ctttttttcg cagtgcgcct ggttctgcct gcggggcgct ccggaggccc aaaggaagct
     5341 gatgtggtgg cccggcctgg tttttgttct ccatattcta gtcagtctgt gccgaatggc
     5401 cgcggccgtt ttcgcccccc atgcgcaggc ggatttgttt cagcaaaaca cgaccattgc
     5461 cctgagcgtg atcgcctacc agcttctgtt tgtcgcgctc actttcgcgc tggcgctcat
     5521 gtacaaccag cggctcgtcg aagaggttcg ggcgaatgaa gaaaaatttg agctggcctt
     5581 tcaatccgct ccttatacgc tcgttttgtc ccgcctgtcc gacggaaaga tcctggctat
     5641 caatgacagt ttcaccagaa tcaccggcta tagcgaagcg gacattcggg gaaagacggc
     5701 ggcggatctg aagctgtggg tcaacgagga agaccgggcc attgttgtcc gggacctgcg
     5761 ggaaaccggc gccgtctttg agcggaaact gctgtttcgg aaaaaatcgg gtgagatcat
     5821 gacggggctc ctttccgcaa aatggattac cattcaggat gaaaagtgcc tgctgttcag
     5881 cgtcaacgac gtcaccgaac gggaaaaggt cctggaagaa cttcgcaccg cgcaggagca
     5941 gtggcagaaa actttcgatt ccacccagga tgccatctgg ctcatggacc ggaatcacaa
     6001 catcctgcgt tcgaataaag ccgcccagcg ccttttcgat cgtccggcgg aaaacatgac
     6061 cgatcgacac tgctgggaag tcgtccacgg cacgtcggcg ccgactcccg aatgccccat
     6121 tttactggcc agggaatccc tgcggcgtga atccatggat ttatccatcg cggggaaatg
     6181 gtatcttgtc acggtggacc cgattctgga caaagaggag cgttttgcgg gcgccgtcca
     6241 caccatgacc gacatcagcg aacgcaggca ggcccaggag gaaatcaacc acctcaatga
     6301 gaccctggaa cagaaaatcg ccgatcagac ccgtgatctg cgcgacagcc aggcggcgct
     6361 tctgaatctg gtggacgatc tcaacgaaaa cgcgaaaaac ctcaccatga tcaatgactc
     6421 cctggaagag gtcaaccggg agctggcgtc cttcagctac agcgtctcgc acgatctgcg
     6481 ggcgccgctg cgcacgatcg acggattcag caacgccctt atggaagact acagcgaccg
     6541 gctggatgcg gaaggtaata aatatctgga aagaatccat cacgccacgc agcagatgaa
     6601 ccggctgatc gacgacctgc tcggcctctc gcgggtgatg aaatccgatt tttaccggca
     6661 ggatttcaat ctgagcgaag tggtacgcga gatttgcgcc gactttcacc agcagccttc
     6721 ggaaggccgg ccggatctgg taattcagga cggcatcgcc gtcaacgcgg atcagcgcat
     6781 gatgcgcatc gtcatgacca acctgctgga caatgcccgg aaattcgcgg ggaaaaagga
     6841 gcggccgatc cttgaattcg gctcttttgt cgaaaacggc gaaacggtct tctttgtccg
     6901 ggacaacggc gtgggattca acatgaccta cgcccaaaga gtttttgagc cttttgaacg
     6961 cctccaccgg gccgatgagt ttccgggaac gggaatcggc ctggcgacgg tcgcacgcgt
     7021 catttcccgc caccgcggac gcatctgggc tgaagcccag gaaggaaaag gagcgacatt
     7081 ttatttcacc ctgggagcct gaaaatgcca aaacccctga acgtgctcat gattgaagat
     7141 gcggaagacg acgagctgct tctgacccgc gcactgaaaa aaaacggcta tgaaccgaaa
     7201 gtcgagcggg tgcagacggc ccgggaaatg acccgggccc tggaaaacag cccgtgggat
     7261 atcatcctgt gcgactacca catgccggcc ttcagcggca tcgaagccat cgacctgctg
     7321 aagaacaccg gcctcgatat tcccctgatc atggtatccg gcgccatcgg cgaagaaacg
     7381 gcgctggact gcatccaccg cggggcgtcg gactatatca tgaagggcaa tctgacgcgg
     7441 ctgggcatgg ccgttaagcg ggggctggaa gaaaaagcat ttcgcgacca tcaccggcag
     7501 gacgaggagg cgctgcgccg aagcgaggaa aagtaccgga caatcctgga aagcatcggc
     7561 gacggctatt acgaaacgga cctggccggc agctttacgt ttttcaacga cgccctgcag
     7621 cgaatatggg gctacccgaa agaagaactg ctgggcctga acaaccggca atacatggac
     7681 ggggaaacgg ccaaaatgct gtatcaggcc cacaaccgga cttacaaaac gggcatcccc
     7741 ggaaaattga tggactacga gatcattcgg aaggacggcg ggcggatagc gatccagcag
     7801 tctttcacgc tgctgaaaga ttccctgggc aacaccatcg gcttccgggg ggtcgtgcgc
     7861 gacatcaccc atctgaaagc gctggagcag gagagggagg aaaccctggg aaagcttcgg
     7921 cagacgctgg agtccaccgt tcacgccatg gcggtggccg tggaatcgag ggatccctac
     7981 acggcgggcc atcagcggcg ggtgacggag cttgcctacg ccattgccgc cacgatggga
     8041 ctggagccga accggatcaa cggtctgcgc atggccagca tgattcatga cctgggaaaa
     8101 atatccattc ccaccgagat tctcaccaag ccgaccaaac tgacgaaaat tgaatttgaa
     8161 atcgtcaaga cgcacgcaga ggcaggatac gaaattctga aggacatcgc cttcccctgg
     8221 cccatcgcgc ggatcgtttg ggagcatcac gagaagatcg acgggtccgg ttatccccgg
     8281 catttaaaag gagacgatct tctgacggaa tcgaaaatcc tgacggttgc cgacgtggtc
     8341 gaagccatgg cgtcccaccg cccctaccgc ccttctctgg gccttcagga tgcgctggag
     8401 gaaatcacgc ggaaccgggg gatcctctac gacgcggatg ccgtcgccgc ctgccttact
     8461 ttgtttgagg aaaagcgttt cagctttgca atataacgag tcgctcgtca tcctcgctca
     8521 tcgctcgctg cttctttatc gtcttgacaa gggtcaaacg cgccggtaca gtacgtcaac
     8581 cgtgaccggt taaagaatac aggaggatta ttcatggctt ccccgaaaag cggcacaacg
     8641 acctctccct ggacggactg gcaaaaacaa ctgaacgagt atctcgtgga ttccgcgcag
     8701 cgctcgatca tttttgcgga cattctgcgc aagagaggca acaattacat caagcacatt
     8761 cgaggcggcc agccgccggt tctgacgttc aactacgaga tgatcctgaa cgccaagcat
     8821 ttcgagcggc cggtcaatta cgcgctggtt cgcatttccg accgccgccg gaaagacatc
     8881 gatcccgagc agcaaaaccg ccgccgcgcg cttcgggaac gccccgacat tcccaaacgg
     8941 ccgattgtca tcatcgatcc gcgggcgggc cacggccccg gcatcggggg ctccaaacgg
     9001 gattcggaaa tcggcatggc actggaccac ggccatccgg tttattttgt tctgttttac
     9061 acggatcccc ttcccggcca gacgctgccg gatgtcatag ccgccattga acggttcatc
     9121 gaaaaagtca cccagctgca ccccgacgcg gatgcgccgg cggtcatcgg caactgccag
     9181 gcgggctggg cggccgcgct ggcctgcgcg gacaagccgg gcatcgccgg ccccctggcg
     9241 ctcaacggcg cgcccctgtc ctactggagc ggtgtcgagg gcgtcaatcc catgcgctac
     9301 cggggaggcc ttctgggcgg cgtgtgggtc aattcatttt tcagcgatct gggcaacggc
     9361 gtgtttgacg gcgcgaatct ggtgctgaac atggagctgt tgaatccggc caacacatac
     9421 tggaccaaac aatacaacgt ttattcaaag gcagacacgg aagaggagcg ctatctcaac
     9481 tttgaaaaat ggtggggcgg cttcttcatg atgaacgacg atgaaattca cttcatcgtc
     9541 cgcaatcttt ttgtcggcaa caagctggag cgaggcgaac tggagctgcg ggaaggccgg
     9601 aaggtcagcc tgaaagacat cgaaagcccg atcctggttt ttgcgtcgat gggcgacaac
     9661 atcacgccgg cccagcaggc gctcaactgg attccccgcg tgtacagctc cgtggaagac
     9721 atcaaacggc acgggcaggt catcgtctat atcgttcacc aggaaatcgg ccacctgggc
     9781 atcttcgtcg ccggctccat cgccaaaaag gagcaccatg aaatcatagg caatttcgac
     9841 atgattgact gcctgccgcc cggcctctac gaaatggtca tcgacggcga tgtcggaagc
     9901 ggcaaattcg aaagccggtt tgaaccgcgc acgatggccg acattgccgc ttacgacgac
     9961 ggaatacacg atgaaacgga tttcagcgtc gtggccaagg tatccgaagc caacgacaat
    10021 ttctatcaac tgctggtgcg gccctttgtg aaactctgga ccacggagct ttccgcggag
    10081 ttcatccgcc atctgcaccc cctgcgcgtg cagcggtatt cgctggccga catcaatccc
    10141 ctgacgcggc cgctgcaaaa cgtcgccaac gtcgtgcgca ggaaccggaa ggcggcgggg
    10201 ccggacaatc cgtttacgga gctggagaag gcggtttccg attatgtgga aggcgtgctc
    10261 gacatttacc gcgattaccg ggacctctac caggaagaaa tttttcagca gatttacggc
    10321 aacggctggc tgcgcagcct gtttccgccc gtccaggacg agccgaagcc tttagccgcc
    10381 aaacaggacc atcccgacta tgacaagcgg ctggccgcca tgaagcaggg aggtttcgcc
    10441 gaaggattga tccgcattta catggccgcg ggcggcacca accagggcat caaaaggaag
    10501 catttcgaag tcgccagtga aatcgccgcc acgcaccggg tattgagcaa actgtccccg
    10561 tcgcagttca aaaaaataat gcgcgaacag gccgccattc ttcaggcgga cgaggaaagg
    10621 gcccttcagg ctcttgcagc gctgatcaaa aacagggacg accgcatgga ggcgctgagc
    10681 atcgcccgcc ggctgtttct ggcggacggc gtttacaacg acgaggaaaa agtgctgctc
    10741 gaaaagatca ggaaggggct gggcctcgaa gctgtataga tgatatacaa agcagaaaag
    10801 gggac
//
LOCUS       DAOPAF010000052        10609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6082_length_10609_cov_49.518950, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000052 DAOPAF010000000
VERSION     DAOPAF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_10609_cov_49.518950"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLQ77_05985"
     CDS             complement(<1..983)
                     /locus_tag="PLQ77_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPL10259.1"
                     /translation="MKFLLIYPKWEKLDRQTRFILPPHGPVVMAAALPSDAEVAFVDE
                     NVEPVDLGADADFVGVSMMLTAQVKRGWEIADAFRARGIPVICGGIAVMLHAEECAKH
                     ADAVFLGEAEGRMEQVFADFQAGKLQKVYNYMQNFPPIENVGTARRGILKREMYNYRG
                     VQMVDLVHASRGCRFNCYPCAVRFLGGRSFRPRPYDRVMEEMASIDNNRLFIVDNSLA
                     QDRDWEIGLFKEMIPLKKKWCCHPIEDDDEVLDLAAQAGAWFVYQTIFDSSDYIRDRI
                     KRYHDHGIAVEGSILLGLDYHTEDYIKRWMDFLLEIELDMAEFTILTPFVHT"
     gene            complement(997..2244)
                     /locus_tag="PLQ77_05990"
     CDS             complement(997..2244)
                     /locus_tag="PLQ77_05990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPL10260.1"
                     /translation="MSEHRDFPDNRRVVITGLGMATPLGTGREEFSRRLFQGESAVDA
                     VQAFDTGAAASHLGAEVRDFQPRDFISIKNLRRMDRLSMLATASARLALSDAGIAVTA
                     ENRDRVGILFGTAFGATDVTVQFEDALLAQGPGAANPILVPNTVMNAPAGYASIELGF
                     RGVNVTVTHFAVSAETAVAYAASEIRRGTADVMLAGGADILSKFYYEALSKFRGLSPD
                     DGGPEGAHPFDERRNGYVAGEGCGLLCLESLACARERGAKICCEITGAGLGSSPAPPT
                     AWPRDPEGFCRAFLRALKQARIDVADVSAVMAAANGSRVLDAVEAAAYEGLFASRAVR
                     PWVTSIKGALGESFSGGGIRACALALSVERGCLPLTVGLERPAAPLNFVAGKKVKTHL
                     KHTVLSGISFGGACACLVFSRCE"
     gene            complement(2237..3481)
                     /locus_tag="PLQ77_05995"
     CDS             complement(2237..3481)
                     /locus_tag="PLQ77_05995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPL10261.1"
                     /translation="MKKRIVITGMGTKNAIGGNVSEFARSLREGVCGISQIDLFDTTD
                     FRTHKGGQVKDFDPRRSIPEEFSLKRISRADALSMAAALEALTDAGLYPLQENIKENM
                     GIAIGGGAGGLLEAESFYADLLRKGKERSKYSQLSSLFCASSADRIASSLEFSGPRAT
                     FMTACSAGATALGYARDLILNGHAGIMLAGGVEPMCRLTYAAFNALKSVDEDVCRPFD
                     RNRSGLSLGEASAVLVLEPLETAQNRGAKIYGELMGYGVTCDSFHMTSPDEKASGAAR
                     SMRAALADGGLTVEDIDYINAHGTATPVNDLTETKAIKEVFGSRAYQIPVSSTKSMHA
                     HTLGASGALEGIVSLLALSKRFVPPTIHWRERDPECDLDYVTQGARRAELRAVLSNSF
                     AFGGNNTTVIFGRYEGQGAIHE"
     gene            3679..4728
                     /locus_tag="PLQ77_06000"
     CDS             3679..4728
                     /locus_tag="PLQ77_06000"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPL10262.1"
                     /translation="MFKTRMTEMFGIRHPIMLAGMNWITEPKLVSAVCNAGGLGILAI
                     ARCNPKETRENIKQIQDLTDKPFGVNQILVGPGAKENIAVAIEEKVPIINYSLGKPWF
                     VNDVHAYGGKVVGTIAIARHAAKAVQLGCDALVITGHEAAAHGAAATSMVLIPIVASM
                     VKVPLIAAGGFYDGRGLAAALALGADGISMGSRFMVVKESMVHENFKRLCMEATEQDT
                     LYDNVFDGMDGRVLKTKEALAMMKRGFPIVEAFKGALLVKRLMNLSFAKFLGLSLEML
                     RAEEGHPLMVLARQAVGNMKHLKALNDGDVEGGILFAGQCCGGITDNPTTKELIDRII
                     REAEEAQARLNSCRT"
     gene            4789..5400
                     /locus_tag="PLQ77_06005"
     CDS             4789..5400
                     /locus_tag="PLQ77_06005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPL10263.1"
                     /translation="MQLTLIRHGETVWNREKRVQGLTDVELSETGIDQARRLALSLRD
                     FPIDVIYSSPLKRAFQTAELINRYHRKAINVEPGLMEMDQGDFEGLTFQELLAREQDF
                     IRSWIADPESVRMPGGESLAELQSRAWPPVEKLMAEGRNALIVSHNFTIATILCRLRN
                     IRLSEFRSTCVDNASRTHIRIEEGAARIEAINDVRHLKTETDV"
     gene            complement(5584..5964)
                     /locus_tag="PLQ77_06010"
     CDS             complement(5584..5964)
                     /locus_tag="PLQ77_06010"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPL10264.1"
                     /translation="MSEGFLSIGELSRKLEMSQRTIRYYEEIGLLNSIKRVEGGRRIY
                     TEKDLRRLKLIKRLKIMGMTLSEMQELESMWSYEKSNEKLLRRLLELLNNHLKRLDDR
                     IADLDILRHEILEYQERISAKINK"
     gene            complement(6019..7572)
                     /locus_tag="PLQ77_06015"
     CDS             complement(6019..7572)
                     /locus_tag="PLQ77_06015"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HPL10265.1"
                     /translation="MSNICYRDTIGDMARRAATKYGDKIAIIFRDEKISFYELERQAR
                     RFANLMTSYGVKKGDRVAIYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAYVVN
                     HSGSRIFIVEDILYPIIAKDQAKFTTVEKWGFLPLGDSLVPDGFFNMITEMEAMTYDE
                     PQVEVSAEDIVQIPYTSGTESKPKGAMLSHRALMSQYHSCIFDGQYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPLEMIRLIEKYQITHMFAPPTVWIGILNHPE
                     FKNYYHSSLKKAAYGASIMPVEIIKQLAVTFRGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVPLGEVGEIVHRSGHVMTGYLNDSEKTSEAFEFG
                     WFHSGDLGRFDADGYLYVVDRKKDMIKTGGENVASREVEEVLYQYPGIEEAAVIGLSD
                     PKWIEIVAAVVVPKKGASLIDKDIIAHCRERLAAFKCPKKVILLSRLPKNPSGKILKR
                     ELKEKFAAS"
     gene            complement(7680..9323)
                     /locus_tag="PLQ77_06020"
     CDS             complement(7680..9323)
                     /locus_tag="PLQ77_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL10266.1"
                     /translation="MSGFVNKTMGDVIEETAGRFPDHPALIAPQFQVRLNYRELYEQC
                     RKTARGLMAMGVRRGDHVSVWTTNVPEWVYLQFALGMVGGILVTINTNYQSHELEYIL
                     RQSDSTTLFLIDQYRDTSFYETVRKVVPELGSCEPGKTASAKLPVLKNVVYIGNRESA
                     PGMFKFSDLIAMGESVSEQELGERMSSLTDDDVINMQYTSGTTGFPKGVMLTHHNVIN
                     NARMVGDVMGLTDKDTLLIQVPVFHCFGCVMSTLNSVYHASTMVLLESFDPLLSLELI
                     HKEKCTAVNGVPTMFIAMLHHPDFERYDMTSLRTGIMAGAPCPVEAMNQVRTKMHCSE
                     VVIAFGQTESSPVMTMTRRDDPVDLRVATVGRLLPDIEGKIVDPETGEDLPPNTQGEI
                     VTRSACIMKGYYKMPEATTAAIDADGWLHTGDLGEIDPNGYFKVTGRIKDMIIRGGEN
                     IYPRELEEFIFTHPKVQNVQVVGIPDSKYGEQVLAAIHLKNGNRATAEEFIEFCKGKI
                     ARHKIPKYWEFVDEYPMTASGKIQKFKIREIFEKKYKAD"
     gene            complement(9660..>10609)
                     /locus_tag="PLQ77_06025"
     CDS             complement(9660..>10609)
                     /locus_tag="PLQ77_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPL10267.1"
                     /translation="GLFGLKPTRGRNPLGPESGEVWQGAEVEGALTRSVRDAAAMLDA
                     TNGADSGAPYILPRPARPYLEETRQDPSRLKIGFTVESPLKNAVHPECVEAVKNAAKL
                     LERLGHKVEEARPDIDGEALGRSYMMLYFGEVAADIRELASVVGRPLTHSDTEEATWA
                     LNLLGKTFSAGDFVLAKRQWNTFARQMAKFHETYDLYLTPTIASLPPRIGEMQQKPAE
                     LAFLKIINGLNLGGLLKATGIADMLATKGLSKMPFTQLANLTGQPAMSVPLHWSREGL
                     PCGVQFVGRFGEESLLFQLAGQLERAQPWFDRRPALTKS"
BASE COUNT         2190 a         3154 c         3025 g         2240 t
ORIGIN      
        1 gtgtggacaa aaggcgtgag aatcgtgaac tccgccatgt ccagctcgat ttccagcaga
       61 aagtccatcc agcgcttaat gtaatcttcc gtgtggtagt ccaggcccag cagaatgctc
      121 ccttccacgg cgatgccgtg atcgtggtag cgcttgatcc ggtcgcggat gtagtcggag
      181 gagtcgaaaa tggtctgata gacaaaccac gcgcccgcct gcgccgccag atccagaacc
      241 tcgtcgtcgt cttcgatcgg atggcagcac cacttctttt tgagcggaat catttccttg
      301 aagagaccga tttcccagtc gcggtcctgc gccagcgagt tgtccacaat gaacaggcgg
      361 ttgttgtcga tggaggccat ttcctccatg acccggtcgt aggggcgggg gcggaagctt
      421 cggccgccca gaaagcgcac cgcgcaggga tagcagttga agcggcagcc gcgggaggcg
      481 tgaaccagat cgaccatctg gacgccgcgg tagttgtaca tctctcgttt gaggatgccg
      541 cgccgtgccg ttcccacgtt ttcaatcggc gggaagttct gcatgtagtt gtaaactttt
      601 tgcagcttgc ccgcctggaa atcggcaaag acctgctcca tgcggccttc ggcttcgccc
      661 aaaaacaccg cgtcggcgtg cttggcgcat tcctcagcgt gcagcatgac ggcgatgccc
      721 ccgcagatga cgggaatccc ccgggcgcga aacgcatccg cgatttccca gccgcgcttc
      781 acctgcgcgg tcagcatcat cgagacgccg acaaaatcgg catccgcgcc gaggtccacc
      841 ggctcgacat tttcatcgac aaacgccacc tccgcgtcgg aaggcaaagc ggctgccatc
      901 accaccggcc cgtgcggcgg aagaatgaac cgcgtctggc ggtccagctt ttcccatttc
      961 ggatagatca gcaaaaattt catgcattcc cctttatcat tcacatcggc tgaaaaccag
     1021 gcaggcacag gcgccgccaa aggatatgcc ggataaaacc gtgtgcttca ggtgggtttt
     1081 cacttttttg cccgcgacaa aattcaacgg tgcggccggc cgttccaggc cgaccgtcaa
     1141 aggcaggcat cctctttcca cagataacgc cagagcgcaa gcccgaattc cgcctccgga
     1201 aaaactctct cccagagcgc ccttgatgga ggtcacccag ggacggacgg cgcgggatgc
     1261 aaataatcct tcgtaagcgg ccgcctccac ggcatccaga accctgcttc cgttggccgc
     1321 cgccatcacg gccgacacgt ccgcaacgtc aatccgcgcc tgcttgagcg cccgcagaaa
     1381 cgcccggcaa aacccttccg gatcccgcgg ccaggccgtg ggcggcgcgg gcgaggaccc
     1441 caggccggcg ccggtgattt cgcagcatat ttttgctccg cgctcacggg cgcaagccag
     1501 ggactccaga cagaggaggc cgcacccttc tcccgccaca tacccgtttc gccgctcgtc
     1561 aaagggatgg gcgccttccg gccccccgtc atccggcgaa agcccgcgga atttcgacag
     1621 ggcttcatag taaaatttcg acagaatatc cgcaccgccg gccagcatca catccgccgt
     1681 cccgcgccgg atttctgagg cggcatacgc cacggccgtc tccgccgaca ccgcgaaatg
     1741 cgtgaccgtg acattgacgc cgcgaaaccc cagctctatg gaggcgtacc cggcgggcgc
     1801 gttcatgacg gtgttgggca ccaggatcgg attggccgcc cccggcccct gcgccaggag
     1861 cgcgtcttca aactgcacgg tgacgtcggt cgcgccaaag gcggtcccga agagaatgcc
     1921 cacgcggtcg cggttttcgg ccgtgacggc aatgcccgcg tcggaaagcg ccaggcgcgc
     1981 cgatgcggtc gcaagcatcg acagccggtc catccgccgc agattcttta tagagataaa
     2041 atcacgcggc tggaaatcgc gcacttccgc gccaagatgc gacgccgccg caccggtgtc
     2101 aaaggcctgc acggcatcga cggcgctctc gccctgaaaa agcctgcggg aaaattcctc
     2161 ccggcccgtg ccgagcggcg ttgccatccc caggccggtg atcaccaccc gccggttgtc
     2221 cggaaaatcg cgatgctcac tcatggattg cgccctgccc ttcataccgg ccgaaaatga
     2281 ccgtcgtgtt gtttccgccg aaagcaaagg aattggatag aacggcgcgc agctcggccc
     2341 ggcgcgctcc ctgggttaca taatccaggt cgcattccgg gtcgcgttcg cgccagtgga
     2401 tcgtcggcgg aacaaatctt ttggacagag ccagcaggct gacgatccct tccagggcgc
     2461 ctgacgcgcc gagggtatgg gcatgcatgg atttggtcga actcaccggg atttgatagg
     2521 cgcgggaacc gaacacctct ttgatggctt tggtttccgt taaatcattg accggcgtgg
     2581 ccgtgccgtg ggcgttgatg taatcgatat cctccaccgt gaggccgccg tccgccagag
     2641 cggcccgcat ggagcgcgcg gcgccggacg ccttttcatc gggcgacgtc atgtgaaagg
     2701 aatcgcaggt gacgccgtag cccatcagct ccccgtaaat tttcgcgccc cgattctgcg
     2761 ccgtctccag aggctccaga accagaacgg ccgatgcctc gcccagggaa agccccgacc
     2821 ggttcctgtc aaaaggccgg cagacgtcct cgtccacgga tttgagggcg ttgaaggcgg
     2881 cgtaagtaag ccggcacatc ggctccacgc cgcccgccag catgatgccg gcgtggccgt
     2941 tcaatatcag gtccctggca tagcccagcg cagtggcgcc cgccgaacag gccgtcataa
     3001 aggtggccct ggggcccgaa aactccaggc tggaggcgat gcggtccgcg gacgaggcgc
     3061 aaaacaaaga agacagttga gaatacttcg aacgctcctt cccttttcga agcaaatccg
     3121 cgtagaagct ttccgcttcc agaagccccc ccgcgccgcc cccaatggct attcccatgt
     3181 tttctttaat gttttcctgt aaggggtaaa ggcctgcatc agtcagagcc tcgagggccg
     3241 ccgccatgga cagagcatcg gcgcgggata ttcttttgag agaaaactct tccggaatcg
     3301 accggcgggg atcaaagtct ttgacctgcc cccccttatg cgtgcggaaa tccgtggtgt
     3361 cgaaaagatc gatctgagag atgccgcaga cgccttcccg aagcgaccgg gcaaactcgg
     3421 aaacgtttcc gccaatggcg ttttttgttc ccatgcctgt aatgacaata cgttttttca
     3481 ctatccatga atctccttgt tcccgctttc ggcaagggca tgcttggcct tttcgagctt
     3541 taaatggaac agtctcaaaa gtcaaatgca tattccggcg ccgcgcacgg cttgaaaaat
     3601 ttgttttgac atcaacccct gcatctccta tgattccccg cgcaaaaaat ttaaaatccg
     3661 acgctgaagg agaatgcaat gttcaagacg cgaatgaccg agatgttcgg catccggcat
     3721 ccgattatgc tcgccgggat gaactggatc acggagccca aactggtttc ggccgtctgc
     3781 aacgcgggcg ggctgggcat tctggccatc gcgcgctgca accccaaaga aacacgggaa
     3841 aatatcaagc aaatacaaga tcttacggat aagccgttcg gcgtcaatca gattctcgtg
     3901 ggaccgggcg caaaggaaaa catcgccgtc gccatcgaag aaaaagtgcc gatcataaac
     3961 tattcgctgg ggaaaccctg gtttgtcaac gacgtgcacg cctacggcgg gaaagtcgtg
     4021 gggacgatcg ccattgcccg gcatgcggcc aaggccgtgc agctcggctg cgacgcgctg
     4081 gtcatcaccg gccatgaagc ggcggcgcac ggcgcggcgg caacctccat ggttttgatt
     4141 ccgattgttg catccatggt aaaggtgccg ctcatcgccg cgggcggatt ttacgacggc
     4201 cggggacttg cggctgcgct cgcgctgggc gcggacggca tttccatggg cagccgcttc
     4261 atggtggtca aggaaagcat ggtgcacgaa aacttcaagc gcctgtgcat ggaggcgacc
     4321 gaacaggata cgctctacga taatgttttt gacggcatgg acggccgcgt gctgaagacg
     4381 aaggaagccc tggcgatgat gaagagaggc ttcccgattg ttgaagcctt taaaggcgcc
     4441 ctgctggtca aacggctgat gaatctgtcg tttgccaaat tcctgggctt gagccttgaa
     4501 atgctgcgcg ccgaggaagg ccaccccctg atggttctgg cgcgccaggc ggtgggcaac
     4561 atgaagcatc tcaaagccct gaacgacggg gacgtggaag gcggcatttt gtttgccggg
     4621 caatgctgcg gcggcatcac cgacaacccg accacgaagg agctgatcga ccggatcatc
     4681 cgcgaggccg aagaggcgca ggcgagatta aacagttgcc ggacataaag agagaatcaa
     4741 ccggcccgcg gccgaagaac atagaaagaa caaagacttc aaaagcttat gcaactgaca
     4801 ctgatccgcc acggcgaaac cgtctggaac cgggagaagc gcgtgcaggg tctcaccgat
     4861 gtcgaactga gcgaaaccgg catcgaccag gcccgaaggc tcgcgttgtc gctcagagac
     4921 ttcccgatcg acgtcatcta ctccagcccg ctcaagaggg cctttcagac ggccgaactc
     4981 atcaaccggt atcaccggaa agccatcaac gtcgaacccg gcctgatgga aatggaccag
     5041 ggggactttg aaggtctgac gtttcaggag ctgctggccc gcgagcaaga ttttatccgc
     5101 agttggattg ccgatccgga atcggtgaga atgccggggg gagaatcgct ggccgaactg
     5161 cagagccgcg cctggccgcc cgttgaaaag ctcatggccg aaggccggaa cgccctgatc
     5221 gtttcgcaca acttcacgat cgcgacgatt ttgtgccgtt tgagaaacat tcggctttcc
     5281 gaattccgca gcacctgcgt ggacaacgcc tcccgaacgc acatccgcat tgaggaagga
     5341 gcagcgcgca tcgaagccat caatgacgtc cgccatttaa aaacagagac ggatgtctag
     5401 aaccgtttca gggtgacttg ctgatgaact cgtaaaaagt ctaaataatg gcaaattcag
     5461 gcggcttcgt aaaaagctcc gcaggcaagg cgcgcaaatc atgaggaatg aggcgtactt
     5521 cagcgtacgc cgcgatgacg aaggatgcag cgcaacgcag catccgggct ttttacgagg
     5581 ccgtcacttg ttgatcttcg cgctgatccg ctcctgatac tccaggattt catggcgcag
     5641 gatgtccagg tcggcgatcc ggtcgtcgag tctctttaag tgattattca gcagttccag
     5701 aagacgcctc agcagtttct cgttggattt ttcgtagctc cacatggatt cgagttcctg
     5761 catttcggaa agcgtcatgc ccatgatctt cagccttttg atcagtttca ggcgacgaag
     5821 gtccttttcg gtgtagatcc ggcgtccgcc ttccacgcgc ttgatggaat tgagcagacc
     5881 gatctcttcg taatagcgga tggtccgctg actcatctcc agctttctgg agagctcccc
     5941 gatggacaaa aagccttcgc tcatgatcgg ctccctttgc ttttcaaagt cacgggcggc
     6001 gtccgcccgc ggccggattt acgacgcggc aaacttttcc ttgagttcgc gctttagaat
     6061 tttcccagaa ggattcttgg gcagtctgga aagcagaatc acctttttgg ggcatttgaa
     6121 ggcggccagc ctttccctgc agtgggcaat gatgtccttg tcgattaacg aagcgccttt
     6181 tttcggcacg accaccgccg ccacgatttc gatccacttg ggatcggaaa gaccgatgac
     6241 ggccgcttct tcgatgcccg gatactgata gagcacttcc tcgacctcgc gggaagccac
     6301 gttctcgccg ccggttttga tcatgtcctt cttgcggtcc accacgtaga gatagccgtc
     6361 ggcgtcaaaa cggcccagat cgcctgagtg gaaccagccg aactcaaagg cttcggatgt
     6421 cttttccgaa tcgttgaggt agcccgtcat gacgtggccg gagcggtgaa cgatttcccc
     6481 cacttctccc agaggaacaa attttccgtc gtcgtccatc agccgcgttt ccacgttcag
     6541 aaccggcttg cccgccgaac ccggcttcag aagctggtct tccggcttca ggatcgtggc
     6601 caccggcccc atctccgtct ggccgtagta gttccacagt ttgaggccgc ggaaggtcac
     6661 cgcgagctgc ttgatgattt ccaccggcat gatgctggcg ccgtaggccg cctttttcag
     6721 gctgctgtgg tagtagtttt tgaactccgg gtggttgagg attccgatcc acaccgtggg
     6781 cggagcgaac atgtgcgtga tctggtattt ttcaatgagc ctgatcatct ccagggggtc
     6841 ggccttgtgc atgattacgt tggtggcgcc gacatacaga tacggcatga gaaaacagtg
     6901 aagctgcgcg cagtgaaaca ggggcagcgc gtgcaggctc acgtcgtcgg tttcatactg
     6961 gccgtcgaag atgcagctgt ggtactggga catcagcgcc ctgtgcgaga gcatcgcccc
     7021 cttgggcttg gattccgtgc cgctggtgta ggggatctgc acaatgtctt cggcgctgac
     7081 ctctacctga ggctcgtcgt aggtcatggc ctccatttcc gtgatcatgt tgaagaaacc
     7141 gtcaggaacc agcgaatccc ccagcggaag gaagccccac ttttcgaccg tcgtgaattt
     7201 cgcctggtcc ttggcgatga tcggatacag gatgtcttcc acgataaaga ttctggagcc
     7261 ggaatgattg acgacgtaag caatttcttc ggcattcagc atgtagttga tgggaacttg
     7321 aatggcgccg atcttggcca ggcccatcat gctgatggga tagtagtgcg aattgtaggc
     7381 gtagatcgcc acccggtcgc cctttttgac gccgtagctg gtcatgagat tggcaaagcg
     7441 ccgcgcctgc cgctcgagtt cataaaaaga aatcttctcg tcgcggaaaa tgatggcgat
     7501 cttgtcgccg tatttggtgg ccgccctgcg ggccatatcg ccgatcgtgt cacgataaca
     7561 aatattgctc atcttgaaat cctttcgctt ttgttctcct gctttatcct atagcccata
     7621 gcctatcgcc tcttgcctgt tttttttcca tccaccatcc actatccacc acagcttctt
     7681 cagtctgctt tatacttctt ttcaaatatc tcacgaatct tgaacttctg aatcttgccg
     7741 ctggccgtca tcgggtattc atccacgaac tcccagtatt tggggatctt gtggcgcgcg
     7801 atttttccct tgcagaattc gatgaattcc tccgccgtcg cccggttgcc gttcttcaga
     7861 tggatggccg cgagaacctg ctcgccgtat ttggaatccg gaatcccgac aacctggaca
     7921 ttttgcacct tgggatgcgt gaagatgaat tcttccagct cccgcggata aatattttcg
     7981 ccgccccgaa tgatcatgtc cttgatccgt ccggtgactt taaaatagcc gttcgggtcg
     8041 atctcgccga ggtcccccgt gtgcagccac ccgtcggcgt cgatggcggc ggtcgtggct
     8101 tccggcatct tgtagtagcc cttcatgatg caggcgctac gcgtgacgat ttcaccctgg
     8161 gtgttcggag gcaaatcctc gccggtttcg ggatcgacga tttttccttc gatgtccggc
     8221 agcagacggc cgaccgtggc cacgcgcagg tccaccggat catcgcggcg ggtcatggtc
     8281 atgaccggcg aggattccgt ctggccgaac gcgatgacga cttcggaaca gtgcattttc
     8341 gtgcgcacct gattcatggc ctctaccggg cagggcgctc cggccataat gccggtccgc
     8401 agggacgtca tgtcgtagcg ttcgaaatcg ggatgatgga gcatggcgat gaacatggtc
     8461 ggcacgccgt tgacggccgt gcatttctct ttgtgaatca actccagaga cagaagcggg
     8521 tcgaaagact ccaaaagcac catggtggag gcgtggtaaa cgctgttgag cgtgctcatg
     8581 acgcacccga agcaatgaaa gacgggcacc tgaatgagca gcgtgtcctt gtccgtcagc
     8641 cccatgacgt cgccgaccat gcgggcgttg ttgatgacat tgtggtgggt cagcatcacg
     8701 cctttgggga accccgtcgt tcccgacgtg tactgcatgt tgatgacatc gtcgtcggtg
     8761 agcgaactca tccgctcgcc cagctcctgc tccgaaacgc tctcccccat ggcgatcaga
     8821 tcggaaaatt tgaacatgcc cggcgcgctt tcccggttcc cgatatacac gacgtttttc
     8881 aacaccggga gcttggcgga cgccgtcttt ccgggctcgc aggaacccag ctccggaacg
     8941 acctttcgga ctgtttcgta aaagctggtg tcccggtact ggtcgatcag gaacagggtg
     9001 gtggaatcgg actgcctgag aatgtattcc agctcgtgag actggtaatt ggtattgatc
     9061 gtcaccagaa tgccgcccac catgcccagc gcgaattgca gatacaccca ttccggcacg
     9121 ttggtcgtcc agacggaaac atggtccccg cgacggacgc ccatcgccat cagcccccgg
     9181 gcggttttgc ggcattgttc ataaagttcg cggtaattga gccggacctg aaactgcgga
     9241 gcaatcagtg ccggatgatc gggaaaacgg ccggccgttt cctcaatcac gtctcccatc
     9301 gttttgttca caaaacctga cataacccac ctcacctgta atatttttca gcgtcacgcg
     9361 gaaccacgcc cggctccggc ttagcgccaa tgtcgcgcga atatccttcg tcggatcact
     9421 taacgtaaac tgggccgact atacgtgatt tatagaaaat gtcaataaaa tcattgaaaa
     9481 ttggcgaaat tcttttttgg ctgagaatct tgacggtccg gaccagacaa atatggaata
     9541 atactgagtt accggctttt cgtggccggg caggcgaaaa ttccgttcat gcaggagcgc
     9601 ctgcccgtct gcggcgttgc ttttccgctt ttgagaagaa gggtgtttga acttccccgt
     9661 cagcttttcg tcagggccgg tctgcggtca aaccagggct gcgcccgctc gagctggccg
     9721 gccagttgaa acaaaagcga ctcctcgccg aagcggccga caaactggac gccgcagggc
     9781 agtccttccc tgctccagtg gagaggcacc gacatggccg gctggccggt gaggttggcc
     9841 agttgcgtga aaggcatttt ggacaggccc ttggtcgcca gcatatcggc gatgcccgtc
     9901 gctttgagca gtccccccag gttcaggccg ttgatgattt tcaggaaagc cagttcggcc
     9961 ggcttttgct gcatttcgcc gatgcgcggg ggcagcgaag caatcgtcgg cgtcagatac
    10021 agatcgtagg tttcgtgaaa tttggccatt tgccgggcaa aagtattcca ctgccgtttg
    10081 gccagaacga agtcgcccgc ggaaaaagtc ttccccagca gattcagggc ccaggtggct
    10141 tcctccgtgt cggaatgggt gagcggccgg cccacgacgc ttgcgagctc ccgaatgtcc
    10201 gctgcaacct cgccgaaata cagcatcatg tagcttctgc ccagcgcctc gccgtcaata
    10261 tccggcctgg cttcttccac cttgtgcccg aggcgctcca gaagcttggc cgcatttttc
    10321 acggcctcga cacattcggg atgaacggcg tttttcagcg gcgattccac cgtgaatccg
    10381 attttcagcc gcgaaggatc ctggcgcgtc tcttccagat acggccttgc cggccggggc
    10441 aaaatatagg gagcgccgga atctgcgccg tttgtggcgt ccagcatcgc ggccgcatcg
    10501 cgcacggagc gggtcagcgc gccctccacc tccgcaccct gccagacttc gcccgattcg
    10561 ggccccagcg gattgcggcc gcgggtgggc ttgaggccga agaggccgc
//
LOCUS       DAOPAF010000053        10528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6141_length_10528_cov_55.346510, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000053 DAOPAF010000000
VERSION     DAOPAF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6141_length_10528_cov_55.346510"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1543)
                     /locus_tag="PLQ77_06030"
     CDS             complement(215..1543)
                     /locus_tag="PLQ77_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPL10268.1"
                     /translation="MKSIDKLKRLTGEDKAPKKTEPVQPDSASSSERQSQLADLRGRI
                     ENVVTRGALRTRAAQEEARARGNRALCELLEGEEIENRHGRFFLAGGFLSGVSRHGHR
                     SICEAFNFDMTAAGMLANNPVLGAYHPSDALFLDTETTGLSGGTGTMAFLIGLGWFEE
                     GRFHIRQILARDFGEEPAALACLQEIAAQKKFLVTFNGKAFDVNLLATRFILNRMQSS
                     LHELPHLDLLHPSRRLLGHRLENCRLATLEADILGVEREGDIPGWEIPQRYFDWLRRR
                     DPRLLADIFEHNRLDVISMATLTAHLVDLLNAQALQQHAHPDDYLAAARLLLKKAGTA
                     GVYKILEILGENACAELSLSSKKNLANLCKRTGRMDEAVRIWRRMLDCDPNDFYAVTE
                     LAKHLEHRARDYGQAKALIEKAMAGDNFFSEEEKDSLEHRLNRLNARKTL"
     gene            complement(1540..3921)
                     /locus_tag="PLQ77_06035"
     CDS             complement(1540..3921)
                     /locus_tag="PLQ77_06035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL10269.1"
                     /translation="MAKLTLDEWIARLKKNKSFNAGAAAVVDIPARPGDWEDYPSWVS
                     PNLRDVLVKRGMERLYRHQAQAVRFIRGGQDVVLVTPTASGKTLCYNLPVLQSILDEP
                     ETRALYLFPTKALAQDQMHEAHSLISDLQADIKTFTYDGDTPDDARRAIRRQGHIVVT
                     NPDMLHAGILPHHTKWQKLFVNLKYVVIDELHIYRGIFGSHFANVIRRLIRICRFYGA
                     NPVFICCSATVANPREHAEKILERDVALLDKSGAPAAAKTFVLFNPPIVNRELGIRQS
                     AMTPARKIASDLIGNHIQTIVFATSRLNVEVLTKYLKDKFVKSKPLDDHFVTGYRGGY
                     LPNLRRDIENGLRTREVMGVISTNALELGIDIGNLEACIMAGYPGSIASTWQQAGRAG
                     RRTGRSLAILIARSNPLDQFIMENPDYFFSLSPEHCRINPDNLLILLHHIKSAAFELP
                     FEKGEKFGGENLEEFLQYLEEKGVLHRTDDRWHWAAESYPADEVSLRAINPENVVVVD
                     ATDAGNHKVIAEVDWDSAFTAVHDEAIYMLASEQYHVDKLDLERKKAYVRKVDSDYYT
                     DAMTYTNVRVIDSFDNFRTGGTIVEHGEVQVVRKVVGFKKIKFYTSENLGYGDVNLPE
                     KDMHTTSYWFTIPRDRLRGLDFTPAEIIDGLSALAYTLQHLSAMFLMADTHDIDRSLG
                     DKSGEWFVSQGKTGRVITSFSGGRPQAINSSDAARFDEFDPTIFIFDSYPGGIGFSEL
                     LYREHANLLAAAKSLIEKCPCAFGCPSCVGPTLEVGKTAKEIVPKIIDLVLNQ"
     gene            4086..4910
                     /locus_tag="PLQ77_06040"
     CDS             4086..4910
                     /locus_tag="PLQ77_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL10270.1"
                     /translation="MALFSIDRDKCRRDGICAAQCPAQIIVQADRQSFPSLLNGAEEF
                     CIRCGHCVAVCPHGAFSLSGMTPDECPPVDPGLLPGADALKHLLLARRSIRRYKKTPV
                     SRKVLAELLDVARCAPTGSNKQQVHWIVYSQQEKVRRLAGMVVDFMKIMLPAAADEAA
                     LRRFRRVVDAWDEGQDRITRDAPHLIIAHSPSDLSFPAADCAIALTYLELYACARGLG
                     TCWAGYFTAAANLHEPLIRELNLPAGRQCCGAVMLGYPLCRYYRIPARNEAQIHWR"
     gene            5332..7311
                     /gene="acs"
                     /locus_tag="PLQ77_06045"
     CDS             5332..7311
                     /gene="acs"
                     /locus_tag="PLQ77_06045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPL10271.1"
                     /translation="MAEANEQDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESITDPN
                     AFWAKMATERLTWFKPFDKNKVSDWSFDAKNVHSKWFEGGKLNVSYNCLDRHLATKKN
                     KAAIIFEGNDPNDWKVYTYFDMYREVNKFANVLKSLGVKKGDRVTIFLPMIVELSIVM
                     LACARIGAVHAIVFGGFSAEALRDRINNSGSAVVITCDGTYRGAKAVPQKDTCDKALT
                     QCPGVKTLVVVKRTGTAVTMKEGRDIWYEELMAKADRYCKPEEMDAEDPLFILYTSGS
                     TGQPKGVVHTQAGYLLYASMTQQLAFDVREEDTYWCTADIGWVTGHSYIVYGPLCNGH
                     SSILFEGVPNYPDFGRFWAVVEKYSVNNFYTAPTAIRSIAKEGDKWVDAHDISSLRVL
                     GSVGEPLNPEAWWWYYNKIGASRCTISDTWWQTETGGHMILPLPGAIDIKPAKAMVPF
                     FGVVPVLFDEEGKEIKGPGKGALCIKNSWPGMARGLWNDTEGRFVENYFVQFPGTYFS
                     GDGAERDADGDYKITGRVDDVINVSGHRLGTAEVEAALTSHADVAEAAVVGFPHDIKG
                     QGIFAYVTLNNNVEKTDELKKALVAHVRKVVGPIASPDQIQWADGLPKTRSGKIMRRV
                     LRNVAAKTFGDFGDTSTLADPHVVDDLVENRKKLG"
     gene            complement(7533..9641)
                     /locus_tag="PLQ77_06050"
     CDS             complement(7533..9641)
                     /locus_tag="PLQ77_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL10272.1"
                     /translation="MEKLFYPQSIAIMGLSSRPDNIPRLVLENLIRWGYLGRIFGVNP
                     RSTDPHVDGIKMYRDIGELPAVPDLVFVLIPARFVPDAVDACGKAGVRWMAIPSGGFN
                     ELGEEGRKLAEQVVRRARQYGIRFVGPNGVTVANTANGLCLPFVPSFSPPKGGLSIIT
                     QSGGVGLMLWNLMTDENVGMAKFASIGNKLDLDEVDFLEYFGRDPDTRVIGMYLESIP
                     RGDRLIEAASRISKPVIVLKSNTTDAGKKVAMSHTAAISNNEAILDAAFERAGITRIH
                     EFNDFISMAKVFDLPPMRGKRIMVMSPAGGFTVMMADLCEESGFAFADPGPAFYDELK
                     NSSNAGVIHFSNPLDMGDIYDAGAYSNIFFSVMHNENVDGAVYVTQWPEMPRGEDVFY
                     KMFHTDLSKEVTGTILSSGKPLGICLFGLSATISRIKRNLDIPIFNNAGEMIRAFKKQ
                     HDYYARKADGSPVHALPDRIDIRAAKTWIADHPGVKGEEALELLRHGGIAAAESVVAG
                     NANEAAAAAERIGYPVVMKIVSPDAVHKSDAGGVITGIETAEAVRSAFDRIRRNLDRC
                     KKDARFEGIRVMKQAGEGYDMFIGGQRDASFGPVVSFGYGGIYIEVFKDVAHVLCPAG
                     SREIEEKIRKLKSCKILQGARGKGAGDIPGYAAAIERVSHLLHQFPQIRELDVNPLRV
                     LADGSGVVALDARIRIVPNP"
     gene            complement(9698..10189)
                     /locus_tag="PLQ77_06055"
     CDS             complement(9698..10189)
                     /locus_tag="PLQ77_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL10273.1"
                     /translation="MALKNGKTVPVRPVLPTDESFIVDLLNKLSSDSIYLRFLRPVKA
                     LPKELLFQLTHLDYQRNFALAAVIREDEKDAIIGVARYGYDPQEQLTDFAIVVRDDWQ
                     HLGLGKQMLSKIFAIGRKQGIGRFVSIVDSTNIAMKRILRELDYPVNYSYEKGFTQVE
                     VLV"
     gene            complement(10281..>10528)
                     /locus_tag="PLQ77_06060"
     CDS             complement(10281..>10528)
                     /locus_tag="PLQ77_06060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPL10274.1"
                     /translation="KNKRCEGEAEYAIIISDPWQNKSLGITLSEQCIELAKKEGYKTI
                     RAETLQENYAMIRIFRRCAFQFDGKDENMVSMTLNLS"
BASE COUNT         2197 a         3009 c         3016 g         2306 t
ORIGIN      
        1 aaagcgaagc cgcgcgccgt ctgggcatca cccgcaaaac cctgcgcaag aagcttacga
       61 agtatgaaga caggcattcc tgagaagttt gatccatttt ggccgggaag tgctgcgatt
      121 cttccattga ataaatctgt tggccggtga agtgcggttt ttaagagggt ctttaaaaag
      181 tttccacgcg ggaaaatagc ggcttgcgca ggtgtcagag agtctttctg gcgttcagac
      241 gattcagtcg atgttcaaga gaatccttct cctcttcgga aaaaaaatta tctcccgcca
      301 ttgctttttc gatgagtgct tttgcctgtc cgtaatccct cgcgcggtgc tccaggtgct
      361 tggcaagttc ggtgacggcg taaaaatcgt tcgggtcgca gtcaagcatt cgccgccaga
      421 ttcggacggc ttcgtccatg cggccggtgc gtttgcagag attcgccagg ttctttttgg
      481 aggataagga aagctcggcg caggcgtttt ccccgagaat ctccagaatt ttgtaaacgc
      541 ccgccgtccc cgcctttttg agcagaagcc tcgccgccgc aaggtagtcg tcgggatgcg
      601 cgtgctgttg gagggcctgc gcattcagaa gatcgaccag atgcgcggtc agcgtcgcca
      661 tcgaaatcac gtccagccgg ttgtgttcaa aaatatcggc gagcagccgg ggatcgcgcc
      721 ttctcagcca gtcgaaatag cgctgcggaa tttcccagcc gggaatgtcg ccttctcgct
      781 caacgcccag gatgtccgcc tccagcgtgg ccaggcggca gttttccaaa cggtgcccga
      841 gcagcctgcg cgacggatgc agcagatcca gatggggcag ttcgtgaagg ctgctttgca
      901 tacggttcag aatgaagcgt gtggcgagca ggttgacgtc aaaggccttg ccgttgaagg
      961 tcaccaggaa ttttttctgc gcggcgattt cctgaagaca ggccagcgcc gccggctcct
     1021 cgccgaaatc gcgcgccaga atctgccgga tgtgaaaccg gccttcctca aaccagccca
     1081 ggccgatgag aaaggccatg gtgcctgttc cgccggaaag ccccgtggtt tccgtgtcca
     1141 gaaaaagcgc gtcggaaggg tgataagcgc ccaggaccgg attgttggcc agcatgcccg
     1201 ccgcggtcat gtcaaaattg aaagcctcgc aaatgctgcg gtggccgtgt cggctcacgc
     1261 cggaaagaaa acctccggcc agaaaaaaac ggccatgccg gttttcgatc tcctcgcctt
     1321 ccagcagttc gcaaagcgcc cggttgccgc gcgcccttgc ttcctcctgc gccgccctgg
     1381 tgcgcagggc cccccgggtg acgacatttt caatcctccc gcgcaaatcg gccagttgcg
     1441 actgcctttc gctggatgat gccgaatccg gctgcacggg ttccgtcttc tttggggcct
     1501 tatcttcgcc ggtcaggcgt tttaatttat caatagattt cattggttca aaaccagatc
     1561 aataatcttc ggcacaattt ccttggccgt tttgcccact tccagcgtgg ggccgacgca
     1621 ggacgggcag ccaaacgcgc agggacattt ttcaatgagg cttttcgcgg cggccagcag
     1681 attggcatgc tcgcggtaga gcagttccga aaagccgatg ccgccgggat aggaatcgaa
     1741 gatgaagatg gtcgggtcga attcatcaaa gcgagcggcg tcggaactgt taatcgcctg
     1801 cggccgcccg ccggaaaacg aagtaatgac tcggccggtt tttccctggc tcacaaacca
     1861 ctcgccgctt ttgtcgccga gcgatcggtc gatgtcatgc gtatccgcca tcagaaacat
     1921 ggccgacaga tgctggagcg tataggccag cgccgaaagt ccgtcaatga tttccgccgg
     1981 cgtgaaatca agcccccgca gccggtctcg cggaatcgta aaccagtagc ttgtggtgtg
     2041 catgtccttt tccggaagat tcacgtctcc atagcccaga ttttccgagg tgtagaattt
     2101 gatctttttg aagcccacca ccttgcgcac cacctgcacc tcgccgtgct cgacaattgt
     2161 cccgcccgtg cggaagttgt cgaacgaatc gatgacgcgc acattggtat aggtcatggc
     2221 gtcggtgtag tagtccgaat ccactttgcg gacgtaggcc ttctttcgct ccagatcaag
     2281 tttgtccaca tggtactgct cggaggccag catgtaaatc gcttcatcat ggacggcggt
     2341 aaacgcgctg tcccagtcca cttcggcaat gactttgtga ttgcccgcgt cggtcgcatc
     2401 gacgaccacg acattttccg ggttgatggc gcgaaggctg acctcgtcgg ccgggtagct
     2461 ttccgccgcc cagtgccagc ggtcgtccgt ccggtggagg acgccttttt cctccaggta
     2521 ctgcaaaaac tcttcgagat tttcgccgcc gaatttttct cctttttcaa acggcagttc
     2581 gaaggccgcg ctcttgatgt gatgaagcag gatcagcagg ttgtccggat tgatgcggca
     2641 atgctcgggc gatagagaaa agaagtagtc gggattttcc atgatgaact gatccagcgg
     2701 gttgcttcgc gcgatcaaaa tagccagaga tcgtcccgtt cgcctgcccg cgcggcccgc
     2761 ctgctgccag gtgctcgcga tcgagccggg atagccggcc atgatgcagg cttccaggtt
     2821 gccgatgtca atgcccagct caagcgcatt ggtgctgatg acgcccatta cttctcgcgt
     2881 gcgcagaccg ttttcaatgt cgcgccgcag gttcggcaga tacccgccgc ggtagccggt
     2941 gacaaagtgg tcgtcgagag gcttgctttt aacgaatttg tctttgagat acttggtcag
     3001 gacttcgacg ttaaggcggc tggtggcaaa gacaatcgtc tgaatatggt tgccgatcaa
     3061 gtcggaggcg atcttgcgtg ccggcgtcat ggcgctctgg cggatgccca gctcgcggtt
     3121 cacgatgggc gggttgaaca gcacaaaggt tttggccgcc gcgggcgcgc cgcttttatc
     3181 cagaagcgcg acgtcccgtt ccagaatttt ttccgcgtgt tcgcggggat tggccaccgt
     3241 ggccgagcag cagatgaaga cgggattcgc gccgtaaaag cggcagatgc ggatcagcct
     3301 gcggatgaca ttggcaaaat gcgatccgaa aatgccgcgg tagatgtgca gctcgtcgat
     3361 gacgacgtat ttgagattga caaacagctt ttgccacttg gtgtggtgcg gcaaaatgcc
     3421 cgcgtgcagc atgtcgggat tggtgacgac gatatgtccc tgccggcgga tggcccggcg
     3481 cgcgtcatcg ggcgtgtcgc cgtcgtaggt aaaggttttg atgtcggcct gcaaatccga
     3541 aatcaggctg tgcgcttcgt gcatctggtc ctgcgccagc gccttggtgg gaaacaggta
     3601 aagcgcccgg gtttccggtt cgtccagaat gctctgcagc accggcaggt tgtagcaaag
     3661 cgttttgccg ctggccgtgg gcgtaaccag cacgacatcc tgcccgccgc ggataaagcg
     3721 caccgcctgg gcctgatgcc tgtagagcct ctccattccg cgcttgacca gaacatcgcg
     3781 aagattcggg ctcacccagg acgggtaatc ttcccaatcc cccggccggg ccgggatatc
     3841 caccaccgcc gccgcgccgg cgttgaagct tttgtttttt ttcagacggg cgatccactc
     3901 gtccagggtc aatttggcca tgcgattttt ccttgttctt tccggcgggc atgatagata
     3961 aaaaaacgaa atatcacaac agagaattag caaaaattca ttgcaataaa caggtgaatg
     4021 atttatctgc ccggtaaggg acgaatcgga acaaaatcaa tcttttgaag aaaaggggag
     4081 aagccatggc gcttttcagc atcgaccggg acaaatgcag gcgcgacgga atctgcgcgg
     4141 cccaatgccc cgcccaaatc atcgttcagg cggaccggca gtcttttccg tctttgctca
     4201 atggcgcgga agaattctgc atccgctgcg gccactgcgt ggcggtttgt cctcacgggg
     4261 ctttttccct ttccggcatg acccctgacg aatgtccgcc ggtcgatccg ggcctgcttc
     4321 ccggcgcgga tgcgctcaaa catctgcttc tggcgaggcg ctccattcga cggtacaaaa
     4381 agacgcccgt ttcccggaag gtgctggcgg agcttctgga cgtcgcccgc tgtgcgccca
     4441 cgggaagcaa caagcagcag gttcactgga ttgtttactc acagcaggag aaagtgcgca
     4501 ggctggccgg aatggtggtc gatttcatga agatcatgct tcccgcggcc gcggacgagg
     4561 cggccctccg ccgcttccgg cgggtcgtgg acgcctggga cgagggacaa gaccgcatta
     4621 cgcgggatgc cccgcacctg atcatcgcgc acagcccctc cgatctttcc tttcccgcgg
     4681 ccgactgcgc gatcgcgctc acctatctgg agctttacgc ctgcgcccgg ggactgggca
     4741 cctgctgggc cgggtatttc acggcagcgg cgaatctgca cgagccgctg attcgggagc
     4801 tgaatcttcc ggcgggccgc cagtgctgcg gcgccgtgat gctcggatac ccgttgtgcc
     4861 gctattaccg tattcccgcg cgcaatgagg cgcagatcca ctggcgctga tgatcgctcg
     4921 atgctcggac ttcatggccg atagcccatc gcctctcgcc ttcggcctgt cctttgtcgg
     4981 acaaacacgg acgcgcctgt aggacatatc ccttcaacag aatcggacgg agactgatac
     5041 caagcgcccc cgaaacagtg tactcagtat gcgcttttca aggggtacat gtattgcatc
     5101 cgccatatgt aatacgcccc gaggttttgc ggtcggtgga tcagaagcgc gcatctgata
     5161 aacaggaaca aggcccgaat cgcggggcgt acccctcctg aaaagactga tgatgtcccg
     5221 tgtcccccct caagtcttga acaatgacac tggatcaccg gttcacaaag agaaatcatg
     5281 cctttttggc ggaaaaggaa ggcataaaaa ataattttga aggagaataa tatggctgaa
     5341 gctaacgaac aagattttta tgacaaggtt tttcctgtgc cggacagagt cagggagaaa
     5401 tcctacatca agagccgggc ggagtatgac aagctttata aagaatcgat taccgatccc
     5461 aatgctttct gggccaaaat ggcaaccgaa agactgacct ggttcaaacc ctttgacaaa
     5521 aataaggtgt cggactggtc gtttgacgcg aaaaacgtcc attccaaatg gtttgaaggc
     5581 ggcaagctca acgtcagcta taactgcctc gaccgccatc tggctaccaa gaaaaacaaa
     5641 gccgcgatca tttttgaagg caacgatccc aacgactgga aagtttacac ctatttcgac
     5701 atgtatcgcg aagtgaacaa attcgccaat gttctcaaaa gtctgggcgt caaaaagggc
     5761 gaccgcgtca ccatctttct gcccatgatc gtcgagctgt ccatcgtgat gctggcctgc
     5821 gcgcgcatcg gcgccgttca tgccatcgtt ttcggcggat tctcggcgga agcgttaaga
     5881 gaccgcatca acaactccgg ttccgcggtc gtcattacct gtgacggaac ctaccgcggc
     5941 gccaaagccg ttccccagaa agacacctgt gacaaagccc tgacgcagtg ccccggcgtg
     6001 aaaaccctgg tggttgtgaa acgcaccgga accgccgtta ccatgaagga aggccgagat
     6061 atctggtacg aagaactgat ggccaaagcg gaccgctact gcaaacccga agaaatggac
     6121 gcggaagatc ccctgttcat tctttatacc tccggctcca ccgggcagcc caagggcgtc
     6181 gttcacaccc aggccggcta cctgctgtat gcctccatga cccagcagct cgcttttgac
     6241 gtgcgcgaag aagacaccta ctggtgcacc gccgacatcg gctgggtcac cggtcacagc
     6301 tacattgtct atggtcctct gtgcaacggc cattccagca ttcttttcga aggcgttccc
     6361 aactatccgg actttggacg gttctgggca gtcgtcgaaa aatacagcgt caataacttc
     6421 tacacggctc ccacagcgat ccgctcgatt gccaaagaag gcgacaagtg ggtcgatgcg
     6481 cacgatattt cctcgctgcg cgttctgggt tcggtcggcg agccgctcaa ccccgaagcc
     6541 tggtggtggt actacaataa gatcggcgcc agccgctgca cgatttccga tacctggtgg
     6601 cagaccgaaa ccggcggaca catgatcctg cctctgcccg gcgcgattga catcaaaccg
     6661 gccaaagcca tggtgccgtt cttcggcgtg gttccggttc tgtttgacga agaaggcaaa
     6721 gaaatcaaag gtcccggcaa aggcgctctg tgtattaaaa attcctggcc cggcatggcc
     6781 cgcggtttgt ggaatgacac ggaaggccgg ttcgtcgaaa attactttgt gcagttcccc
     6841 ggcacgtact tctccggcga cggcgcggaa cgcgacgccg acggcgacta caaaatcacc
     6901 ggccgtgttg acgacgtcat caatgtttcc ggtcaccggc tgggcaccgc ggaagtcgaa
     6961 gcggcgctca cctcccatgc ggatgtcgcg gaagcggccg ttgtcggatt cccgcatgac
     7021 atcaaaggtc agggcatctt cgcttacgtc acgctgaaca acaacgtgga aaagacggat
     7081 gaactcaaaa aggccctggt cgcccatgtc cgcaaggtcg tcggtccgat tgcatcgccc
     7141 gaccagattc aatgggcgga tggtctgccc aaaaccagat ccggcaagat tatgcgccgc
     7201 gttctgagaa acgtcgccgc gaaaacgttc ggtgacttcg gcgacacgtc aaccctggcg
     7261 gatccgcatg tggttgatga cctggtcgaa aacaggaaga aactcggcta aagacccctt
     7321 gtgcggaacg gctgccgttc cgcatgtaag tccctcctta aaaagccgtt ccccggtttc
     7381 ggggaacggc tttttgcttt ggccggatca gatcggaccg gattttccgt attcccttgc
     7441 cctcctgatg cccgggctct ttccgggcat agatccgcct ttccttcagg caaaccgccc
     7501 tgcgtcagga cgcttccgtt cgggcataac cattatgggt tgggaacgat tcgtatcctc
     7561 gcatccagcg cgacgacgcc ggagccgtcg gccaaaaccc gcagcgggtt gacatccagt
     7621 tcgcggatct gaggaaattg atggagcaga tgggagaccc gttcaatggc ggccgcgtag
     7681 ccgggaatat ccccggcgcc tttcccccgg gcgccctgca ggatcttgca ggatttgagt
     7741 ttccggattt tttcttcaat ctcccggctg ccggcggggc acaggacatg ggcgacgtcc
     7801 ttaaacactt cgatgtagat gccgccgtat ccgaaagaaa caaccggtcc aaaggaagca
     7861 tcgcgctgcc cgccgatgaa catgtcgtat ccttcgccgg cctgtttcat gacgcggatt
     7921 ccctcgaacc gggcgtcttt tttgcagcga tcaagattcc ggcgtatgcg gtcaaaggcg
     7981 cttcggacgg cttcggcagt ttcaatcccc gtgatgacgc cgcctgcgtc tgatttgtgg
     8041 acggcgtcgg gcgagacgat tttcatcacc accgggtagc cgatgcgctc ggccgcggcg
     8101 gcggcctcgt tcgcgtttcc ggccacgacg gattcggcag cggcgatgcc gccgtggcgg
     8161 agcagctcca gggcttcctc gcctttgacg cctggatgat ccgcgatcca ggtcttcgcg
     8221 gcccggatgt cgattcggtc tggcaaagca tggacgggcg aaccatccgc ttttctggcg
     8281 taatagtcat gctgtttttt aaaggcgcgg atcatttcgc cggcgttatt gaagatggga
     8341 atgtcgagat tccgcttgat ccgggaaatc gtcgccgaaa gtccgaacag gcagatgccc
     8401 agcggctttc ccgaagacag aatggttccc gttacttcct tggagaggtc cgtatggaac
     8461 attttgtaaa aaacgtcctc tccccgcggc atttccggcc attgcgtgac gtagacggct
     8521 ccgtcgacgt tttcgttgtg catgacggag aagaaaatat tggaatacgc gccggcatca
     8581 tagatgtcgc ccatgtcgag ggggttggaa aaatggatga ccccggcgtt ggaagaattt
     8641 ttgagttcat catagaaagc cgggccggga tccgcgaacg cgaagccgga ctcctcgcag
     8701 agatccgcca tcatgaccgt gaatccgccc gcgggggaca tgaccatgat ccgcttgccg
     8761 cgcatgggag gcagatcaaa caccttggcc atcgagatga aatcgttgaa ctcgtgaatc
     8821 cgggtaatgc ccgccctttc aaaggccgca tcgagaatgg cttcgttgtt ggaaatcgcc
     8881 gccgtatggc tcatggcgac ttttttgccg gcatcggtcg tgttggattt caggacgatc
     8941 acgggtttgc tgatccggga cgcggcctcg atcaggcggt ctccccgggg gatgctttcc
     9001 aggtacatgc cgatgaccct ggtgtcggga tcgcggccga aatattccag gaagtccacc
     9061 tcgtccaggt ccagtttgtt gccgatgctc gcaaatttcg ccatcccgac gttttcatcg
     9121 gtcatgaggt tccagagcat caaacccacg ccgccgctct gggtgatgat ggacagtcct
     9181 cccttgggcg gactgaagga cggcacaaaa ggcaggcaca ggccgttggc ggtgttggct
     9241 accgtgacgc cgttgggtcc gacgaaccgg atgccgtatt gacgggcccg gcgcactacc
     9301 tgctcggcaa gtttccggcc ttcttccccc agttcattga atccgccgga agggatggcc
     9361 atccatcgaa cgccggcttt tccgcaggca tcgacggcat cggggacaaa ccgcgcggga
     9421 atcaaaacaa aaacaagatc cggcaccgcc ggcagttctc cgatgtccct gtacatcttg
     9481 atgccgtcca cgtgcggatc ggtggatcgg gggttgacgc cgaagatgcg cccgagatac
     9541 ccccagcgga tgaggttttc cagaacaagg cggggaatgt tgtcgggcct cgacgacagg
     9601 cccatgatgg cgatggattg cgggtaaaaa agtttttcca taatattttc cgtttgacgg
     9661 tttccgaaga gatggcgtcc gggcggatga ggttctccta taccagcacc tccacctggg
     9721 tgaatccctt ctcgtaggaa tagttcaccg gatagtcgag ttcccggagg atccgcttca
     9781 tcgcgatgtt ggttgaatcg acgatggaca caaaccgacc aatcccctgc tttcggccga
     9841 tcgcaaatat ttttgacagc atctgtttgc cgagacctaa atgctgccag tcgtcgcgca
     9901 ccacgatcgc gaagtccgtg agctgctcct ggggatcgta gccataccgc gccactccga
     9961 tgatggcgtc tttttcatcc tcgcggatga cggccgccag cgcgaagttt ctttgataat
    10021 cgagatgcgt gagttggaag agcagctcct tcggcagagc cttgacgggc ctcaaaaaac
    10081 gcagatagat cgaatcggac gaaagcttgt tcagcaaatc cacgatgaaa gattcatccg
    10141 tcggcaaaac aggcctgacc ggcactgttt tcccgttttt cagggccata cgggtttcgt
    10201 attgcaaaag atcgctccgg ttcattgttg atggcatttt cattacccaa ccggcggcag
    10261 cgcgcggtct gccgccttgc tcacgacaga ttcagcgtca tcgacaccat gttttcatcc
    10321 ttgccgtcga actggaaggc gcagcggcgg aagatgcgga tcatggcgta attttcctga
    10381 agcgtttccg cccggatggt tttgtatcct tcctttttgg ccagctcgat gcactgctcc
    10441 gacagggtaa tccccaggga tttgttctgc cacggatcgg aaatgatgat ggcgtattcc
    10501 gcttctccct cgcagcgctt gtttttga
//
LOCUS       DAOPAF010000054        10370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6247_length_10370_cov_97.964227, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000054 DAOPAF010000000
VERSION     DAOPAF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6247_length_10370_cov_97.964227"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLQ77_06065"
     CDS             <1..1181
                     /locus_tag="PLQ77_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPL10275.1"
                     /translation="CMSQTVTPYLEMSQAIMEAGGEALKKCYQCGTCSGTCPWTPITH
                     FNIRKLVRYGQLGLDGIEDFMWGCSTCRFCVERCPRGVELIDIVTAIRNVYSGGGLLP
                     QSLRAFVGSLSARGNPWSGDPAVRNDWAKGKYPVYKPGMDYLFWTCCTVCYDPRNTRL
                     AKAAAEILNLAGASWGMPSADVNCCGESLRKVGDAELFGRLKGKNLDYFKSNEVSKII
                     TVSPHCLATFNNDYGQDYDVTHLSELMAQWIKDGKLVPKKDFGGLTVTYHDPCYLGRH
                     SKVYDAPREILKALPGVEFVEMERNSEESMCCGGGGGGLWTEKAKGERLSDLRIEEAM
                     GTGAKVLATACPYCITMFEDSARTLNVDGRIQIRDVTELLLESLEINIDEPVGDACAV
                     "
     gene            1308..3257
                     /locus_tag="PLQ77_06070"
     CDS             1308..3257
                     /locus_tag="PLQ77_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPL10276.1"
                     /translation="MSRIGVFVCHCGLNIAKTVRVSELAQFASTLPDVVVAKDYKFMC
                     STPGQEMIANDIREHRLDRVIVTACSPLMHEQTFRKVLAASGLNQYLLTIVNIREQVS
                     WVTHDLDEGTAKAKALLNGAVSRSRLLMPLTSRVIDVNPDVMVLGGGIAGIQAALELA
                     NTGRKVYLVEKNSTIGGHMAQFDKTFPTLDCSACILTPKMDSVLQHKNIQLMTSCEVS
                     EVKGFVGNFDITIRQKARYVDHDKCNACLACTEKCPGKSVSEWDEGLVKRKAIYIPFP
                     QAVPQKPVIDREACTFFKKGKCRLCEKVCEQKAIDFGEQDTFSTVKVGAVIVATGYDL
                     MDTRDLIQYGYGKYPGVYNALEVERLFNSAGPTSGKVTLRDGSEPKAIAILHCIGSRD
                     KNSHEHCSRVCCMYSLKFAHLFKEKTSADVYQFYIDIRAAGKGYEEFYNRIIEEDVKF
                     IRGKVARVAKSVDEPDRLIVEAEDTITGKFIKLPVDMVVLSPAMIPRSNAKDVARLFN
                     LSTDKQGFFMERHPKLAPLSTMSEGVFIAGVCQSPKDIPDTVAQANGAAAEALTIVVK
                     DKMELEATTVMVNPASCCACQNCVRVCPYGAPFFKEDKGVSEINEALCKGCGLCASVC
                     PTGAIIARHFTNDQVLAEMEGLMEF"
     gene            3412..3825
                     /locus_tag="PLQ77_06075"
     CDS             3412..3825
                     /locus_tag="PLQ77_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPL10277.1"
                     /translation="MSFEPKILGIACNWCTYTGADLAGTTRLQYPQNLRVVRVMCSSR
                     INPSFIFRAFQLGADGVLVGGCHPGDCHYNTGNLYARRRLAVTKKILEFAGLEPGRFR
                     VEWISASEGNKFAEVVTEFTGQIKALGPQTKFKIS"
     gene            3842..4708
                     /locus_tag="PLQ77_06080"
     CDS             3842..4708
                     /locus_tag="PLQ77_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10278.1"
                     /translation="MAVSFQQIEEKLKTEARRLLESGQVSLVLAWGRGFDEKHPMPYV
                     AKGASDVDGIVFNEFCTHNLARYLVRYPRGTKMAVAVKAADSRAVVQLIQEDKVKRHD
                     LVILGIPVYGIKNPKTGEIIDGKTTCGLYNPVLYDILIAEEVHGQPVSSPYAVLAEYE
                     AMDQDARFAFWKKELDRCIRCYACRKACPMCYCDPCFIDQNKPKWGEKSPETPGNLMY
                     HLTRFHHLAGRCIDCGECSRACPVDIPLYLFHKKVAKECEEMFGQAAGMSIEDKPVLV
                     NFRMEDSDKILE"
     gene            4721..5758
                     /locus_tag="PLQ77_06085"
     CDS             4721..5758
                     /locus_tag="PLQ77_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10279.1"
                     /translation="MLKKTAIHQLSDLTSALMGKGTLVAPVREDAGFNFREISDAKQV
                     DLNFHNTIISPKSVFFPQTEDFIRYRTGRPLQEAETVEPEGKPLILFGVRPCDVKSFE
                     IMDIHFSCTGAVDPYWQARREAATLIGYAFDVAAPAEPEEFYQTLGIGAADPEGSDIF
                     MVRRGDDLILKSITGKGEALLAGIEGLAGASADDGKFFDDTVAAGRNFKTRFTCVDAK
                     AITKKLEDLFHNTDFWEKASNACLSCGVCTFACPTCYCFDICDETLFGRGTRRRVWDA
                     CMFTDFTLEASGHNPRTKVYQRLRQKIGHKYSYHVRKYGVISCVGCGRCTRHCPVGID
                     IFSIVEEAMKA"
     gene            5809..6669
                     /locus_tag="PLQ77_06090"
     CDS             5809..6669
                     /locus_tag="PLQ77_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPL10280.1"
                     /translation="MRSTNPYIPMPVEIVKITDEVDTHDIKSFRFKFLNAQDEARFQY
                     LPGQFAELSIYGKGESPIGIASSPTEPGYVAFTVQRAGAIVPGLVTAALHDMEEGGRI
                     GLRGPLGHPWPIEFLEKKNIVVVGGGFAFTTLRSLVTYMLHENNRSRFGDITVIYGAR
                     TPGLLCYKDELAAWEKRSDIKTIITVDKGNETWKGREGFVPAVCKDVAPSPANAVAVI
                     CGPPVMIKFTLPVFFELGFSKENILTSLEMRMKCGIGKCGRCNVGYKYVCSDGPVFSL
                     AELEELPRDF"
     gene            6711..7112
                     /locus_tag="PLQ77_06095"
     CDS             6711..7112
                     /locus_tag="PLQ77_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10281.1"
                     /translation="MKKVMAILTMIVFVAGPALAADKAFQKADKNKDGKITSQEYLNA
                     TSKAFDELDKNRDGVLTKEEIQANDKIDADKLIRETDANRDGKILKKEYQKAAKQRFH
                     SMDKNKSGHIDYNEWASDRSDLYSPFNLFSF"
     gene            7294..9477
                     /locus_tag="PLQ77_06100"
     CDS             7294..9477
                     /locus_tag="PLQ77_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPL10282.1"
                     /translation="MRYTVQPLQQGYTNQILSINVTTGKMEVRPLPPEVRNFFIGGRG
                     LGLYLLHEQVTSRTKASDPENPLIFSPGPLGGIPQFPGTSKCMAISLSPLTGIPGVSN
                     FGGHFGAYLKYAGFDALTVAGTAREPVMIVIDGFKREISIVGVSEDDQVFHLEKTLVD
                     RFLREGHEKKDLAFLTTGIGAAHTSFGCINSHYYDATKPVPGGKGLFRTKQAGRTGLG
                     SVMSHKRIAAIVVLTAFPRGENPYGAADWNKVKKAGAKLHGIVRDVDPQALKMHLKGS
                     AGLISFMNKDEYQSLPVYNYQRGSHPQADQVCGKIYAQRLFEHRGMDGCFPGCNLRCT
                     KGGWVTLTSGEHRGQRVWVDGPEYETAAGFGSNLGMWHPEFIMEANWHCDNYGIDTIT
                     TAVLMAFIMECGQRGYLTGEDTDGFSLTWGDEQAALQFIHDLAYRRTELARAAARGMV
                     GLTGWIADKYAARTARTKPLEELQAFAMQTKGLPFSLYRTHRSLSMQASYAAASDIGA
                     HHAAAWLVKVDLLGAFPTFESKARALITYPRVRLGNDNLGLCKLPWVDVFNPDSMKQG
                     HADIYINPASQELYADFYNGMLGTNLTWEQIFEQTDRDINLQRVMNVLFFQSATGDRD
                     WIPDRAIGPTEDALYELEKDYNDGQLSVILNLPLPEIQRMPTGEKRAILMRHRKDELR
                     QLILVYYRERGWTDSGVPKVETLEQIGVWRFLTQEARTIIARLNG"
     gene            9505..>10370
                     /locus_tag="PLQ77_06105"
     CDS             9505..>10370
                     /locus_tag="PLQ77_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL10283.1"
                     /translation="MLQNQVWQPLPGSRQAEIYPYLRKPDLLSSNSCIIRTPRQILLI
                     DAGALAAQTADLNRILTDCQRERSRPVVVYLTHCHLDHSLEVGRHRQIMTTAPVWIAI
                     QEQGADYLSEGDSAKTIAELYGVAFPSLRPDIRLLTETDKRRKAPRSISLMQGVGMTI
                     MTEAIHANLEQPMFQQTISIGGGDVLEIYSAPGHSPDSICIRAGEVLFIGDLLAAANP
                     MVAGISGWHREHLIETQKHVQWLLDHKPVRICYPGHGGLIPSGKVRDILQRLERKTLS
                     LGDVSRMNEERL"
BASE COUNT         2293 a         2915 c         3077 g         2085 t
ORIGIN      
        1 gttgcatgtc acaaacggtt accccctatc tggagatgtc ccaggcgatc atggaggcgg
       61 gcggcgaggc gctgaaaaaa tgctaccagt gcggcacttg ttccggaacc tgcccctgga
      121 cgccgatcac ccatttcaat atccgcaagc tcgtgcgcta cggccagttg ggcctggacg
      181 gcatcgagga tttcatgtgg ggctgctcca cctgccggtt ttgtgtggaa cgctgtcccc
      241 ggggcgttga actcatcgac atcgtgacgg ccattcgcaa cgtgtacagc ggcggcggcc
      301 tgctgccgca gagcctgcgc gcctttgtcg gctccctttc ggcccgcggc aatccctggt
      361 ccggcgaccc ggccgtgcgc aatgactggg ccaagggcaa atatcccgtt tacaagcccg
      421 gcatggacta cctgttctgg acctgctgca cggtctgtta cgacccccgc aacacaaggc
      481 tcgcgaaagc ggcggcggag attctgaatc tggccggcgc aagctggggg atgccttctg
      541 ctgacgtcaa ctgctgcggc gagagcctgc gcaaagtggg cgacgcggag cttttcggac
      601 ggctcaaggg caaaaacctc gactacttca aatccaacga agtttccaaa atcattacgg
      661 tatccccgca ctgtctggcg acgttcaaca acgactacgg ccaggactac gatgttacgc
      721 atttgagcga actgatggct caatggatta aggacgggaa actcgttccc aaaaaagatt
      781 tcggcggcct aaccgtgacg tatcacgatc cgtgctacct gggccggcac agcaaggtgt
      841 acgacgcgcc ccgcgagatt ctcaaggccc ttccgggtgt ggagttcgtc gagatggagc
      901 gcaacagtga agaaagcatg tgctgcgggg gcgggggcgg cggattgtgg acggagaaag
      961 ccaaaggcga acgcctgagc gatttgcgca tcgaggaagc catggggacc ggcgcgaagg
     1021 ttctggcgac cgcctgcccg tactgcatca ccatgtttga agacagcgcg cggacgctga
     1081 atgttgacgg acggattcaa atccgggatg tgacggagct cctcctcgaa agtctggaaa
     1141 tcaacatcga cgaaccggtc ggagacgcct gcgccgtttg atatgagagc gggcggacca
     1201 tcggcgcgga gctcctgaat ggacggggcc gcggggccgg tgttttaaga cagaacggaa
     1261 acggcccgga cgggcggttt ttcaaggaaa gaaagctagg agaaggtatg agcagaattg
     1321 gtgtttttgt ttgccactgc ggattgaata tagcgaaaac cgtgcgggta agcgaactgg
     1381 cgcagtttgc ctccaccctg ccggatgtgg ttgttgcaaa ggattacaaa ttcatgtgct
     1441 ccacgcccgg ccaggagatg atcgcgaacg acatcaggga acatcggctg gaccgcgtga
     1501 tcgtcacggc ctgttcgccg ctcatgcacg aacagacatt ccgcaaggtg ctggcggctt
     1561 ccggcctcaa tcagtacctg ttaaccatcg tgaatatccg cgagcaggtt tcctgggtga
     1621 cgcatgatct ggacgaagga accgccaagg ccaaggcgct gctcaacggc gcggtgagcc
     1681 gctcccggct gctgatgccg ctcacctcgc gcgtgatcga cgtcaatccc gatgtcatgg
     1741 tgctgggagg cggcattgcc ggcatccagg cggcgctgga gctggccaat actggcagaa
     1801 aagtatatct ggttgaaaag aattccacca tcggcggcca catggcgcag tttgacaaga
     1861 cctttcccac gctcgactgc tccgcctgca ttctcacgcc caagatggac agcgtgcttc
     1921 agcacaagaa cattcagctc atgacatcct gtgaagtgag cgaggtcaag ggctttgtcg
     1981 gaaacttcga catcaccatc cgtcagaagg cgcgctatgt cgatcacgac aaatgcaacg
     2041 cgtgcctggc gtgtacggag aaatgccccg gaaagagcgt gtccgaatgg gatgaaggtc
     2101 tggtgaagcg caaggccatc tacattccgt tcccgcaggc cgtgccgcag aagccggtca
     2161 tcgaccgcga ggcctgcacg ttctttaaaa agggcaagtg ccgcctctgc gagaaggtct
     2221 gcgagcagaa ggccatcgat ttcggtgagc aggatacctt cagcaccgtg aaagtcggcg
     2281 cggtcatcgt ggccaccggg tatgacctca tggatacgcg ggatctgatt cagtacggct
     2341 acggcaaata tcccggcgtt tacaacgcgc tggaagtgga gcggctcttc aactccgccg
     2401 ggcccacgag cggcaaggtg acgctgcgcg acggcagcga acctaaggcg attgccattc
     2461 tgcactgcat cggcagccgc gacaagaaca gccacgaaca ctgcagccgc gtctgctgca
     2521 tgtattcgct gaaattcgcc catttgttca aggaaaaaac gtccgctgac gtctatcagt
     2581 tttacattga tatccgcgcc gccggcaagg gctatgagga attttacaac cgcatcattg
     2641 aagaggacgt gaaattcatc cgcggcaagg ttgcccgcgt ggcgaaaagc gtggacgaac
     2701 cggaccgcct gattgtggaa gcggaagaca ccatcacggg caagttcatc aaactgcccg
     2761 tggacatggt tgtgctgtcg ccggccatga ttccccgctc gaatgccaag gacgtcgcgc
     2821 ggctgttcaa tctgagcacg gacaagcagg gttttttcat ggagcgccat cccaagctgg
     2881 cgcctctgtc cacgatgtcg gaaggcgtct tcattgccgg cgtgtgccag agtccgaagg
     2941 atattccgga cacggtggcg caggccaacg gcgcggcggc ggaagccctg accattgtcg
     3001 tcaaagacaa gatggagctc gaagccacga cggtgatggt caatccggcc tcctgctgcg
     3061 cctgccagaa ctgcgtgcgc gtctgcccct acggcgcgcc gttcttcaag gaggacaaag
     3121 gcgtatcgga aatcaatgag gcgctgtgca agggctgcgg gctttgcgcg tccgtctgcc
     3181 cgacgggggc gattatcgca aggcatttta cgaatgatca ggtgctggcg gagatggaag
     3241 gattgatgga attctagtta tggcatcatt aaaaggccgg atactgcgtt gcacatcgtc
     3301 tcgcgtcact gcggcgtatg aaaatatacg cctcgttcca cgatactcgt gcgccttgtc
     3361 tgcggcgctt tttctgacgc cataattatt ttaaaaggaa aggaaacgtt catgtcattt
     3421 gaaccgaaaa tactgggcat tgcctgcaac tggtgcacct ataccggggc cgaccttgcc
     3481 ggaacgacga gactgcagta tccgcagaat ctccgggtcg tccgcgtgat gtgttccagc
     3541 cggatcaacc cgtcgttcat cttccgcgcg ttccaactgg gcgcggacgg cgtgctggtc
     3601 gggggatgcc atccgggcga ttgccattac aatacgggga acctctatgc gcgaagacgc
     3661 ctggccgtga ccaaaaaaat tctggaattt gcaggcctgg agccgggccg gttccgcgtg
     3721 gaatggattt ccgcctccga aggcaacaaa tttgccgagg tcgtcacgga gttcaccggt
     3781 cagattaaag cgctgggacc ccagaccaaa ttcaagattt catagaagta cgagggagca
     3841 tatggcagtc agctttcagc aaattgaaga gaaattaaag accgaagccc gaaggctttt
     3901 ggaatcgggc caggtgagcc tggtgctcgc gtggggcaga ggttttgatg aaaaacatcc
     3961 catgccctat gttgccaagg gcgcctccga tgtggacggc atcgtgttca acgagttctg
     4021 cacccacaat ctggccagat acctggtccg gtatccgcgg ggaaccaaaa tggccgtcgc
     4081 cgtcaaggcg gcggacagcc gcgccgtggt tcaactgatt caggaagaca aggtcaagcg
     4141 gcacgacctg gtgatcctcg gcattcccgt ttacggcatc aaaaatccga agacggggga
     4201 gatcatcgac ggcaagacca cctgcggcct gtacaacccc gtgctgtatg acattctgat
     4261 tgccgaagag gtgcacggac agccggtctc gtcgccttac gccgtgctgg ccgaatacga
     4321 ggcgatggat caggacgcgc gcttcgcgtt ctggaaaaag gaattggatc ggtgcatccg
     4381 ctgctacgcc tgccgcaagg cctgcccgat gtgctactgt gatccctgct ttatcgatca
     4441 gaacaaaccc aaatggggag aaaagtctcc ggagacgccg ggcaacctga tgtaccatct
     4501 gacccggttt catcatctgg ccggccgttg catcgattgc ggcgaatgtt cgcgcgcctg
     4561 tccggtggac attcccctgt atctgtttca caagaaagtg gccaaagagt gcgaagaaat
     4621 gttcggccag gccgcgggga tgagcatcga agacaagccc gtgctggtca atttccgcat
     4681 ggaggattcc gacaaaattt tggaataagg agcttttgtt gtgctgaaga aaaccgccat
     4741 ccatcaactg tccgatctga cttccgcgct gatgggaaag ggaacgctgg tcgcaccggt
     4801 ccgggaagac gccggcttca atttccggga aatatccgac gcgaagcagg tggatctgaa
     4861 tttccacaat acgatcatca gtcccaaatc cgtcttcttt ccacagacgg aagatttcat
     4921 ccgctatcgg acgggcagac ctttgcagga ggccgaaacg gtcgagcccg aggggaagcc
     4981 cttgattctt ttcggcgtcc gcccctgcga cgtgaagagc tttgagatca tggacatcca
     5041 tttttcctgc accggcgccg tggatcctta ctggcaggcc cgaagagaag cggccaccct
     5101 gatcggatac gcttttgatg ttgccgcgcc ggccgagccg gaggaatttt atcagacgct
     5161 gggcatcggc gcggccgacc ccgaaggcag cgacatcttc atggtgcgcc gcggcgatga
     5221 tctgatcctc aaaagcatca ccggcaaggg agaggcgctg ctggccggaa tcgaaggcct
     5281 ggccggggcg tcggccgatg acgggaaatt ttttgacgac acggtcgccg cagggcgaaa
     5341 tttcaagacg cgttttacct gcgtggacgc caaagccatt acgaagaaac tggaagatct
     5401 ttttcataac acggatttct gggagaaggc gtccaacgcc tgtttgagct gcggcgtgtg
     5461 cacctttgcg tgtccgacct gctattgctt tgatatttgc gatgaaaccc tgtttgggcg
     5521 gggaacgcgc cgccgcgtgt gggacgcctg catgttcacg gatttcacgc tggaagccag
     5581 cggccacaat ccccgcacga aggtgtatca gcgcctgcgg cagaaaatcg ggcacaagta
     5641 ttcctaccat gtccggaaat acggcgtcat ttcctgcgtg ggctgcgggc gctgcacgcg
     5701 gcactgtccg gtgggcatcg atattttttc gatcgtcgaa gaggccatga aggcatgatg
     5761 ccgaacgcta aaaagcacgt tcgggaataa accaatttga ggaaagatat gcgttcaacg
     5821 aatccctaca tccccatgcc ggtggaaatt gtcaaaatca ccgatgaagt ggatacccat
     5881 gacatcaagt cgttccgctt taaatttctg aatgcccagg acgaggcccg gtttcagtat
     5941 ctccccggcc agttcgccga gttgtccatt tacggaaaag gagagtcccc gatcggcatc
     6001 gcttcgtcgc ccacggagcc cggctatgtg gcgtttacgg tccagcgggc gggcgccatc
     6061 gttcccgggc tggtgacggc tgcgctgcac gatatggaag agggcggcag aatcggcctg
     6121 cgcgggccgc tggggcatcc ctggccgatt gaatttctgg agaaaaagaa catcgttgtg
     6181 gtcggcggcg gatttgcttt taccacgctg cgctcgctgg tcacctatat gctgcacgag
     6241 aacaaccgtt cccgcttcgg cgacatcacc gttatttacg gtgcgcggac cccggggctg
     6301 ctctgctata aggatgaact ggccgcctgg gaaaagcgaa gcgacatcaa aaccatcatt
     6361 accgttgaca agggtaatga aacctggaag ggccgggaag ggtttgtgcc cgccgtctgc
     6421 aaggacgtcg cgccgtcgcc cgccaatgcc gtggccgtca tctgcggccc gcccgtcatg
     6481 atcaaattca cgctgccggt tttctttgag ctgggttttt ccaaggagaa cattctgacg
     6541 tcgctggaaa tgcgcatgaa gtgcggcatc ggcaaatgcg gccgctgcaa tgtcggctac
     6601 aaatacgtct gttcggatgg gccggtgttt tcgctggcgg agctggaaga gctgcctcgc
     6661 gatttctaga gatgcccgtc gtaaagcaaa tgacgagggt gaggtaaaaa atgaagaaag
     6721 tcatggccat cctgacgatg atcgtttttg tcgccggacc ggcgctggcc gcggacaaag
     6781 cgtttcaaaa agccgataaa aacaaagacg gcaaaatcac aagccaggag tacctgaatg
     6841 cgacttccaa ggcctttgac gaactggaca agaatcgcga cggcgttctg acgaaagaag
     6901 agattcaggc caacgataag attgacgcgg acaagctgat tcgagaaacg gacgcaaacc
     6961 gggacggcaa aatcctgaaa aaagaatacc agaaggcggc aaaacaaaga tttcattcca
     7021 tggataaaaa caagagcggc cacattgact acaacgaatg ggcgtccgac cgctccgatc
     7081 tttactctcc tttcaatctc ttttcgttct gaccgtcgcg actgccctcc gcgggacgca
     7141 aatcccggat ttgggacggg ccggaagccg tccgtccgaa agcggcgccg ggggcttttc
     7201 gcagagggtc gcgcattctt caacaccttt ttatgattga ttttttgcat ggattgcatt
     7261 aacgtgcgcc gactattttc aaaggaacag caaatgcgct atacggttca accgcttcag
     7321 cagggataca ccaatcagat cctttccatc aacgtaacga ccggcaagat ggaggtccgt
     7381 cctcttcctc ccgaagttcg aaatttcttt atcggcggaa ggggtctggg gctttatctg
     7441 ctgcatgagc aagtgacttc ccggaccaaa gcctctgatc cggaaaatcc cctgattttc
     7501 tcaccggggc cgctgggcgg cattccgcag tttcccggaa cgagcaagtg catggcgatt
     7561 tccctgtcgc cgctgaccgg cattcccggt gtctccaatt ttggaggcca tttcggcgct
     7621 tatctgaaat atgccgggtt tgacgcgctg acagtcgcgg gaaccgcccg tgagccggtc
     7681 atgattgtca ttgacggttt caaacgggaa atctccatcg tcggcgtctc cgaagacgat
     7741 caggtttttc accttgagaa aacgcttgtg gaccggtttt tgcgcgaggg ccacgagaaa
     7801 aaggacctgg cctttttgac gaccggcatc ggagcggcgc atacgtcttt cgggtgcatc
     7861 aacagccatt actatgacgc cacgaagccg gttcccggcg gcaaaggcct tttccgcacc
     7921 aaacaggccg gccgcacggg tctggggtcc gtgatgagcc ataagcgcat cgcggccatc
     7981 gtcgttctga ccgcttttcc ccgcggagaa aatccttacg gggcggccga ctggaacaag
     8041 gttaaaaagg cgggggcgaa actgcacggg attgtccggg acgtcgatcc gcaggcgctg
     8101 aaaatgcatc tgaaaggttc agccggtctg atttctttta tgaacaagga cgagtaccag
     8161 tccctgcctg tgtataatta ccagcgcggc tcacacccgc aggcggatca ggtctgcggc
     8221 aaaatttacg cccagcggct gttcgaacac cgcgggatgg acggatgctt tccgggctgc
     8281 aatcttcgct gcaccaaggg cggctgggtc acgctgacat ccggcgagca tcgcggacaa
     8341 agggtctggg tggacggccc cgagtatgaa accgccgcgg gattcggctc gaacctgggc
     8401 atgtggcatc ctgaatttat catggaagcc aactggcact gcgacaacta cggcatcgat
     8461 accattacca cggccgttct catggcattt atcatggaat gcggccagcg cggatacctg
     8521 acgggagaag acacggacgg ctttagcctg acgtgggggg atgagcaggc ggcgctgcag
     8581 ttcatccatg atctggcgta ccgccggacc gaattggccc gcgccgccgc ccggggcatg
     8641 gtgggcctga ccggctggat cgccgataaa tacgcggcgc gaaccgcaag aaccaagccg
     8701 cttgaggagc tgcaggcctt tgccatgcag accaaaggcc ttcctttttc cctgtatcgc
     8761 acgcacaggt ctctatccat gcaggcttcc tacgcggcgg ccagcgacat cggcgcgcac
     8821 cacgcagccg cctggctggt caaagtggat ctgctgggcg cctttcccac gtttgaatcc
     8881 aaggccagag cgctcatcac ctatccccgc gttcgcctcg gcaacgacaa tctgggcctg
     8941 tgcaagctgc cctgggtgga tgttttcaat cccgattcca tgaagcaggg ccatgccgac
     9001 atttacatca atccggcgtc gcaggaactg tatgcggatt tctacaacgg catgctgggc
     9061 acgaatctga cctgggagca gatcttcgaa caaacggacc gggacatcaa tctgcagcgc
     9121 gtgatgaatg tcctgttttt tcaatccgcg accggggatc gcgactggat tcccgaccgg
     9181 gccatcgggc ccacggagga cgcgctctat gaactggaga aagattataa cgacgggcaa
     9241 ctttccgtga tcctgaattt gccgctgccg gaaattcagc gaatgcccac gggcgaaaag
     9301 cgcgccattc tgatgcggca tcgaaaagac gagctgcgtc agttgattct tgtctattac
     9361 cgcgagcggg gctggacgga ttcgggcgtc ccaaaggtgg agacgcttga gcaaatcggc
     9421 gtatggcgtt ttctgaccca ggaagccagg acaatcatcg cccgcctgaa cgggtgaaga
     9481 ttcacggctg gaaaggagtc tcccgtgctt cagaatcagg tctggcagcc tcttccggga
     9541 agtcggcagg cggaaatcta tccgtatctg cgcaagcccg atcttctctc ctccaattcc
     9601 tgcatcattc gcacaccccg gcagattctt ttgatcgacg ccggggcgct ggccgcgcag
     9661 accgccgacc tgaaccggat cctgacggac tgtcagcggg aacgatcccg accggtcgtc
     9721 gtttacctga cccactgtca tctggaccat tccctggagg tcggcaggca ccggcagatc
     9781 atgacgacgg ctcccgtctg gatcgccatc caggaacagg gggccgatta tctgagtgag
     9841 ggagattcgg caaaaaccat cgcggaactc tacggcgtgg cttttccgtc cctgcggccg
     9901 gatatccgcc tgcttacgga aaccgacaaa cgaagaaaag ccccccgttc catttcgctg
     9961 atgcaaggcg tgggcatgac catcatgacc gaggcgatcc acgcgaatct ggagcagccg
    10021 atgttccagc agaccatctc gatcggtgga ggcgacgtcc tggaaatata ttcggcgccc
    10081 ggacacagcc ccgacagcat ctgcatccgc gccggcgaag ttctctttat cggcgatttg
    10141 ctcgccgcgg ccaatccgat ggttgccgga atttccggct ggcaccggga gcatctcatc
    10201 gaaactcaaa agcacgtgca atggcttttg gatcacaagc cggtcaggat ctgctatccc
    10261 ggacacggcg gcctgattcc gtccgggaaa gtccgcgaca tcctgcagcg gctggagcgc
    10321 aaaaccctct cgctgggcga cgtgagccgg atgaacgagg agcgcctctt
//
LOCUS       DAOPAF010000055        10277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6316_length_10277_cov_44.399139, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000055 DAOPAF010000000
VERSION     DAOPAF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_10277_cov_44.399139"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1185
                     /locus_tag="PLQ77_06110"
     CDS             94..1185
                     /locus_tag="PLQ77_06110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10284.1"
                     /translation="MAKYLYNEEAVANLKLGDTSVLDRLVDFESAREAHLKKEHAKKD
                     KRMTIGEAIATFVKDGDIITDGGFSYVRTPHQAFYEIIRQGKKNLQMIGAPNTNQSFF
                     ITYGLVTHSHSSYTGAEMRGIDKVYDRALKAGKFTILSEWSHGGVAQGFKAAQLGVPG
                     FFSKQMLGSDLVRYNPYLRIVQHPLKAAKDPAVFVPALYPDVSIIHCHAADKYGNAYI
                     YGPAVNDLAVAAAARKLIITAEEIVPENDIRYNKQGQIIPFFYADAVVEMPFGALPGS
                     MPGCYYWPRQWWEKCMRFGGSSEENNLAFCNEWILGTKDQYEFIEKLGGARWIAEARR
                     QTKAAEGDNEDIDFSYDEYTLTHDPGRYY"
     gene            1199..1987
                     /locus_tag="PLQ77_06115"
     CDS             1199..1987
                     /locus_tag="PLQ77_06115"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10285.1"
                     /translation="MRDVNAPANPLETLAYIIAQQIEDHSIVYIGTGIPMVAGILAKK
                     THAPNITLVYESGGQDPIEGDMPWSVGCPMTWRKSPTIMEMSYSFAQCYNGYVDIGFL
                     GFAQVDMYGNINTHMIGKDYHHPKVRLTGSGGNNDLSSLTEKMVLVGLQTPDKFPEKV
                     DFATSVGWLKGGDSRKEAGLIGNGPAAVISPWGVYDFHPVSKRMRVKSLTPGVNFELA
                     QQLTGFELLKPEGAIPETKLITPDCLKVIRTEVDPRGVFTTMPS"
     gene            2113..2697
                     /locus_tag="PLQ77_06120"
     CDS             2113..2697
                     /locus_tag="PLQ77_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPL10286.1"
                     /translation="MISRIDHVSIAVRDQEKEKAEHFFRDILGAVAGAGMADPVSRFF
                     WQIYSLGDLSRLEIISPTEPGSFLDGFLSQRQAGVHHITLQTPDIRKAMAHLEAHGIP
                     YFGFNEYPGGVWKEIFIHPRHGFGVLIQIAEFEADDWLAEQVRFPAGTKWQVEKTENG
                     AALIFAHPGGGRVSLELGREDLTRLAKEIRELPG"
     gene            complement(2752..3837)
                     /locus_tag="PLQ77_06125"
     CDS             complement(2752..3837)
                     /locus_tag="PLQ77_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL10287.1"
                     /translation="MAKSRNRKTLTALSLQGGGALGAYEYGVLKALYEKRGKGFTPKV
                     VAGVSIGAINAALLVGAKGDPLTALDRLWRERLCVSWPSLPDCSWAGLTPWSANSLAE
                     RNLSFFANSGMYCLKPEFFLMPALAWLKNSSLYDTSFLRETLADFVDPEKLNRPEKTR
                     LIVTAVNVKTGQLSRFDNSRMELTFDHIIASGSFPVTFPMTQIGEDFFWDGGVFMNMP
                     VGEAVNALEQIEADNPDVEREVIVIELHRMQADLPKTIPEAAERFCNLIFSGKFALDR
                     KLYDRYGAFVDLMGEIDRTIPADSPIRSHKGYKMMTRHRKIDRAMVIGEEGTGATGSG
                     GDFSRNALNRRIEAGYGDAMAFFKKNR"
     gene            complement(3865..5292)
                     /locus_tag="PLQ77_06130"
     CDS             complement(3865..5292)
                     /locus_tag="PLQ77_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPL10288.1"
                     /translation="MIRKISILFFILLIAGCAVGPDYRRPDIQAPTEWRFEDKEARTL
                     INARWWEQFNDPVLNGLIETALKENKDVLIAAARIEEYEGRYAAARGDLFPQAQAAGS
                     ATRQRSTEAGASPWPAGVSNPYPAYQTTLNASWEIDLWGKLRRGTEAARADLLSRENA
                     HRAVLLSLTANVANAYINLRDYDRQLEIAKETLKTREASLEIYELRFDAGVISDLELS
                     QMRSEYENTRATIPQIEKSIHQQENALNLLLARNPGPVVRGLTIDQLNFPHVPEGLPS
                     ELLERRPDIRQAEQDLIAANARIGVARAAYFPAISLTGTLGSASADLSNLFTGPAYIW
                     NAGASVTVPIFTAGRTWGQVKASEALQKQALLTYQKTIQTAFREVEDSLVDQNRTRAK
                     LEALFNQLTALRNYRNLALLRYENGYSSNLEVLDAERNLFSIEITYIETRGGLFNAFI
                     SLYKAMGGGWGVDGSPPPKAPAAEK"
     gene            complement(5294..8476)
                     /locus_tag="PLQ77_06135"
     CDS             complement(5294..8476)
                     /locus_tag="PLQ77_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HPL10289.1"
                     /translation="MISHFFIDRPVFASVISIVIVIAGLVAMSNLPIAQYPDITPPVV
                     TVSATYPGASADVISENIAAPIELQVNGADNMIYMSSTSSSSGMMNLSVYFEIGTSPD
                     LAQVDIQNRVNQALPQLPQSVQQQGVIVSKKSSSFLMVVGIYSPNDRYDHQYVANYAN
                     LYILDAIKRIPGASQAAIMGMPDYAMRIWLKPDRLIQLGITASDVANAIKNQNQQFAV
                     GKIGQAPTKGRVEQTFSVTTKGRLIEPAEFENIILRVNAQGAAMVRLKDVGRAEFGAK
                     DYGTRCRLNGKDATIIAVFQQAGANSLDVSKQVEKTLKELKKGFPDGIDYKIAMDTTK
                     FVRASIKEVVITFFEACLLVIVVVFLFLQSIRATVIPILAIPVSIIGTFIGMTLFGFS
                     INMLTLFGMVLAIGIVVDDAIVVIENVERNMREFNLSPKDAAKKAMEEVTGPVVAIVL
                     VLCAVFIPVAFLGGITGQLYKQFAITIAMSVVFSGIVALTLSPAMAALLLKPRHGEKN
                     RFFRTFDAKFAGLTEGYSRGVSLIIKRVAVALCIFAVMLTCVFVLFRHVPTSFVPSED
                     QGYLLGVALLPDAASLDRTQDLNQKVRTIYAGHPAVENVVEVDGYSLLDGESKTNAGL
                     QFISLNDYEKRKPKELRAPAIIDKTSKLFLGLREGFVMPMNPPSIPGLGVTAGFEFWI
                     QNRGSGDMSKLESLTKELVARAEKQPELQGVATLFNVHSRQLLVDVDREKSENLGVPV
                     EDVYNTLQILFGSSYVSQFTKFSRLWQVILQAEPEYRSRPSDMDQIYVRSRSGSMVPL
                     KALVSQKNVTGPDLVTRFNNFPAAKVIGGPAPGYSSGDAIAAMERVAAEVLPQDFSYA
                     WSGESFEEKKSGGTTVVVFIFGLIMVFLILAAQYEKWSLPFGVLMAVPFAIFGALVAI
                     WLRGQENDIYFQIGLITLIALAAKNAILIIEFAVLKRQEGMSAFDAAVSASKMRLRPI
                     VMTSLAFILGCVPLAVASGASANSRHSIGTGVIGGMLGATLIAVFFIPMFYFLLEKLT
                     ERLGRSGNKKLQSGAAPTATTGETPAGEGK"
     gene            complement(8490..9716)
                     /locus_tag="PLQ77_06140"
     CDS             complement(8490..9716)
                     /locus_tag="PLQ77_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL10290.1"
                     /translation="MDGTGRSHRSFFFLVFCIGWFLIFAAAFSGCGDKSDKEHSRPAI
                     PVTVMTVAPRDVPIDIEFVGTTESSHQVEIRARVEGFLEKKTYEEGGRVRSGQTLFQI
                     DRRPFEASLQQARGALAQQEAKLVNAEATLKRVRPLAEKNAVSQKDLDDAVSAEKSAR
                     AAVFAAQGSVQDAELKLSYTTITSPIDGLAGRAKQQEGSYISVGQASLLTYVAKIDPI
                     WVDFSISENENLKLMDHLAKGLFKMPKGSDFEVKVQMADGSIFPHKGRLNFADPSYNS
                     ETGTYEVRAEIPNPINSPTVIRPGQFVRVHLLGGIRPNGILIPRTAVNQGAQGFFVWI
                     AGKDGKAEFRAVEVGDWQGNDVFINSGLAAGDRIILDNLLKMSPGAPLKIDTSPVAKG
                     AAPVTDQKSPAPPVKK"
     gene            complement(10042..>10277)
                     /locus_tag="PLQ77_06145"
     CDS             complement(10042..>10277)
                     /locus_tag="PLQ77_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10291.1"
                     /translation="SVPSLTNQAIQNLKDTSVAFLIQYHDFFAQMQEVASTNFQFFKA
                     YVTAGAVYLALISIIVLAARRLEKRIALPGLAT"
BASE COUNT         2194 a         2793 c         3102 g         2188 t
ORIGIN      
        1 acccttgact ggagaggggc gaaatagtag tgattttaca gcataaaaat gtgagggaac
       61 acattgggtt aatacagtga ggaggataga gagatggcaa aatatctcta caacgaggag
      121 gccgtagcga atctgaaact tggcgataca tccgttctgg atcgcctggt ggattttgaa
      181 tcagctcgcg aggcgcattt aaaaaaagaa cacgccaaga aggataagcg gatgacgatt
      241 ggcgaggcaa tcgccacctt cgtgaaggac ggggacatca ttaccgacgg aggtttttcg
      301 tacgtaagga cgccgcatca ggcgttttac gagatcatcc gccaggggaa aaagaacctg
      361 cagatgatcg gcgcgcccaa caccaatcag agttttttca ttacgtacgg actggtgacg
      421 cattcccaca gctcctatac gggagcggaa atgcgcggaa ttgacaaggt ttatgaccgg
      481 gcgctcaagg ccggcaagtt cacgattctt tccgaatgga gccacggcgg cgtggcccag
      541 gggttcaagg ccgcgcagct cggcgttccg ggatttttca gcaagcagat gctgggcagc
      601 gacctggtcc gctacaaccc gtacctcagg atcgtccagc atcccctgaa ggcggcaaag
      661 gatccggcgg tgtttgttcc ggccctttat ccggacgtct cgatcatcca ctgccacgcg
      721 gcggacaagt acggcaacgc ctacatttac ggtcccgccg tgaacgatct ggcggtggcg
      781 gcagcggccc gcaaactgat tattacggcc gaggaaatcg ttcccgaaaa cgacatccgc
      841 tacaacaagc aggggcagat cattccgttc ttttacgcgg acgcggtggt cgaaatgccc
      901 ttcggcgcgc ttcccggcag catgcccggc tgctactact ggccgcggca gtggtgggaa
      961 aagtgcatgc gcttcggcgg ctcgtccgag gaaaacaacc tggccttctg caatgagtgg
     1021 atcctgggga ccaaagatca gtatgagttc atcgaaaagc tcggaggcgc ccggtggatc
     1081 gccgaggcgc gccgccagac gaaagccgcc gagggcgaca acgaagacat cgatttctcg
     1141 tacgatgaat acacgctgac ccatgacccg ggccgctatt attaagaagg aggaaattat
     1201 gcgagacgta aacgcaccgg ccaatccgct ggagacactg gcctatatta tcgcgcagca
     1261 gatcgaagac cattcgattg tttatatcgg cacgggaatc cccatggtgg cggggattct
     1321 ggccaagaaa acccacgccc ccaacattac gcttgtgtat gaatccggcg ggcaggaccc
     1381 gatcgaaggc gatatgccct ggtcggtcgg ctgtcccatg acctggcgca aatcccccac
     1441 catcatggag atgtcctatt cgttcgcgca gtgctacaac ggctacgtgg acatcgggtt
     1501 tctgggattc gcccaggtgg atatgtacgg caacatcaac acccacatga tcggcaagga
     1561 ttaccatcat cccaaagtcc gcctgaccgg cagcggcggc aacaatgatc tgtcttcgct
     1621 taccgagaag atggttctgg tcgggctgca gacgcccgac aagtttcccg agaaggttga
     1681 tttcgcaacc tcggtcggct ggcttaaagg cggcgactcc cgcaaagagg cgggcctcat
     1741 cggcaacggc cccgccgcgg tgatcagccc ctggggcgtc tatgattttc atccggtcag
     1801 caaacgcatg cgggtcaaat cgctcacccc cggcgtcaat ttcgagctcg cccagcagct
     1861 caccggtttt gagcttctca agcccgaggg agcgattccc gaaaccaaac tgattacgcc
     1921 cgattgcctc aaggtcatcc gcaccgaggt ggatccgcgg ggcgtcttta ccaccatgcc
     1981 gagctgacag cggacggaac aaaccggccg gaaagaaaga ttccgccgca gactgaaacg
     2041 agatcgcggg cccgcacgca aagggcccgc gattttttca tggctggaat tttatcgagg
     2101 agatgaaaaa aaatgatttc acgcattgac catgtttcca tcgcggtgag ggatcaggaa
     2161 aaggagaagg ccgagcattt tttccgcgac attctggggg cggttgcggg ggccggcatg
     2221 gccgaccctg tttccaggtt tttctggcag atttattccc tgggcgatct ctcccgcctg
     2281 gagatcatct cgcccacgga gcccgggagt tttctggacg gctttctgag ccagagacag
     2341 gcgggggttc accatatcac gctgcagacg ccggatatcc gaaaggcgat ggcgcatctg
     2401 gaagcccacg gaattcccta tttcggtttc aatgaatatc cgggaggcgt ctggaaggaa
     2461 atcttcattc atccccgcca cggcttcggc gttctgattc aaattgcaga attcgaagcc
     2521 gacgactggc ttgccgagca ggtccggttc ccggcgggga cgaaatggca ggttgagaaa
     2581 acggaaaacg gcgcggcgct gatcttcgcc catccgggcg gcggcagggt ttccctggaa
     2641 ctggggcggg aggatctgac gcggctggca aaagagattc gggagctccc cggctgagcc
     2701 ccgcaaacgc tgcgcagcgg gcgggcgagg cttttctgcc gtggaccgga cctagcggtt
     2761 ttttttgaaa aaagccatgg cgtcgccgta accggcttca atgcgccggt tcagggcgtt
     2821 tcgtgaaaaa tcgccgccgg aaccggttgc gcccgtgcct tcttcgccga tgaccatggc
     2881 gcggtcaatc tttcggtggc gggtcatcat tttgtagcct ttgtggcttc ggatcgggct
     2941 gtcggcggga atggtccggt cgatttcacc catcaggtcc acgaaagctc catagcggtc
     3001 atacagtttc cggtccagag cgaattttcc cgaaaagatc agattgcaga agcgttcggc
     3061 ggcctccggt atggttttcg gaaggtcggc ctgcatgcgg tggagttcga tgacgatcac
     3121 ttcccgctcg acgtcgggat tgtccgcttc gatctgttcc agggcgttca ccgcttcccc
     3181 gaccggcata ttcatgaaga cgcccccatc ccagaaaaag tcttcaccga tctgcgtcat
     3241 gggaaaagtg acgggaaaac ttccgctggc gatgatgtgg tcaaaggtca gctccattcg
     3301 gctgttgtcg aaacgcgaca actggccggt tttgacgttc acggccgtca cgatcaggcg
     3361 cgttttttcc gggcgattga gcttttccgg atcgacgaaa tccgcgagtg tttcccgcag
     3421 aaacgatgtg tcgtagaggc tgctgttttt cagccaggcg agcgcgggca tgaggaagaa
     3481 ttccggtttc aggcaataca tgccggaatt ggcaaagaag gaaaggttcc gttcggcgag
     3541 gctgtttgcg gaccaggggg taaggccggc ccaggagcaa tcgggaaggg aaggccagga
     3601 tacgcataac cgttcccgcc agagccggtc gagggctgta agcggatcgc ctttcgcgcc
     3661 gaccagcagc gcggcattga tggcgccgat ggacacgccg gccacaacct ttggggtgaa
     3721 gcctttgccg cgcttttcgt aaagcgcttt gagtacgccg tattcatacg ctccgagcgc
     3781 gccgccgccc tgcaaagaca gagccgtcag cgttttgcgg tttcgtgatt ttgccatgac
     3841 ctttccatgt gcctgtcaaa aagtttattt ctccgccgcc ggggctttcg gtggaggaga
     3901 tccgtccacg ccccacccgc cgcccatcgc cttgtaaagg ctgatgaaag cgttaaaaag
     3961 accgccgcgt gtttcgatgt aggtaatctc gatgctgaaa agattgcgct ccgcgtcgag
     4021 gacttccagg ttgctgctgt agccgttttc atagcgcaga agcgcgaggt tccggtagtt
     4081 tcgcagggcg gtcaactgat tgaacagagc ctcaagcttg gcccttgtcc ggttttgatc
     4141 cacaagggaa tcctccactt cccggaaggc cgtctggatc gttttctggt aggtcagcag
     4201 agcctgtttc tggagcgcct cggacgcctt tacctgtccc caggtgcgcc cggcggtaaa
     4261 aatgggaacg gtaacggatg cgccggcatt ccagatataa gccggacctg taaaaagatt
     4321 ggaaagatcc gcgctggctg agcccaacgt tcccgttagg gaaatggcgg ggaaataggc
     4381 cgccctggcg acgccgattc tggcgttggc ggcaatcagg tcctgctcgg cctgacggat
     4441 gtcggggcgc cgctccaaaa gttcggaagg cagcccttcg gggacatggg gaaaattcaa
     4501 ctgatcgatg gtcaggcccc ggacaaccgg acccggattg cgggccaaga ggaggttcag
     4561 ggcattttcc tgctggtgga tgctcttctc aatctgggga atcgtggcgc gggtattttc
     4621 gtattcggag cgcatctggc ttaactccag atccgaaatg acgccggcgt cgaagcgcag
     4681 ttcataaatt tccagggatg cttcacgggt tttaagtgtt tctttcgcaa tttcgagctg
     4741 gcggtcataa tcccgcaggt tgatgtaggc gttggcgaca tttgccgtga gggacaaaag
     4801 cacggcgcgg tgggcattct cccgggaaag gagatccgcc cgggccgctt cggtcccgcg
     4861 ccgcagcttt ccccagagat caatttccca actggcgttg agcgtggtct gataggcggg
     4921 ataaggattg gaaacgccgg cgggccaggg cgacgctcct gcttccgtgg aacgctgcct
     4981 tgtggcggac cctgccgctt gcgcctgggg aaagagatcg ccccgggcag ccgcatagcg
     5041 gccctcatac tcctcgatcc gcgccgccgc gatgaggacg tctttgttct ccttcagggc
     5101 tgtttcaatg agcccgttga ggacgggatc gttgaactgc tcccaccagc gcgcattaat
     5161 cagcgttcgc gcctccttgt cctcaaagcg ccattcggtc ggcgcctgga tgtccggacg
     5221 ccggtaatcc ggaccgacgg cgcagccggc gatgaggagg atgaaaaaga gaatggatat
     5281 cttcctgatc attttatttc ccctccccgg ccggggtttc cccggttgtt gccgttggcg
     5341 cggcgccgct ctgcaatttt ttgtttcccg atcttcccaa acgctccgtc agtttttcca
     5401 gaaggaagta gaacatcgga atgaagaaaa cggcaatcag tgtcgcgccc agcattcctc
     5461 cgatgacgcc cgtgccgatg gagtgccggc tgttggccga ggcgccgctg gcgacggcca
     5521 gggggacgca gccgagaata aaggccagag aggtcatgac gatggggcgc aggcgcattt
     5581 tcgaggccga aaccgctgca tcaaaggcgc tcatcccctc ctggcgcttc agtacggcaa
     5641 attcgatgat gagaatggcg ttttttgcag ccagagcaat cagcgtgatc agaccgatct
     5701 ggaaatagat gtcattttcc tggccccgaa gccagatggc gacgagcgcg ccgaagatgg
     5761 cgaaaggtac ggccatcagg acgccgaagg gcagcgacca tttttcatac tgggcggcca
     5821 gaatgagaaa caccatgatc aggccgaaga taaagaccac taccgtcgtc ccgcccgatt
     5881 tcttttcttc aaaggactct ccgctccatg cgtaggagaa gtcctgagga agaacttccg
     5941 ccgcgacccg ctccatggct gcgatggcgt ctcccgaact gtaaccgggg gcggggccgc
     6001 caataacctt ggcggcgggg aaattattaa accgcgtaac cagatcgggg ccggtgacat
     6061 ttttctgcga gaccagggct ttcaagggca ccatactgcc gcttctggat cggacataga
     6121 tctgatccat atcgctggga cgggagcggt attccggctc cgcctggagg atcacctgcc
     6181 acaggcggct gaatttcgtg aactgactga cgtaggagga accgaagagg atctggagcg
     6241 tgttgtaaac gtcttccacg ggaacgccca gattttccga cttttcccgg tccacatcca
     6301 ccagaagctg ccgggaatgg acgttaaaaa gggtcgctac accctggagt tcgggctgct
     6361 tttccgcccg ggcgaccaat tcttttgtga gggattccag tttggacatg tccccggacc
     6421 cgcgattctg tatccagaac tcaaacccgg cggtaactcc caggccgggg atcgacggcg
     6481 ggttcatcgg catgacgaat ccttcccgaa gtcccaaaaa caatttggat gttttgtcga
     6541 tgatcgccgg agcgcggagc tctttgggtt ttcgtttctc gtagtcattg agggaaatga
     6601 actgcaaacc cgcattggtt ttgctttctc cgtccaggag gctgtagccg tccacttcca
     6661 cgacgttctc aacggctgga tgcccggcat aaatcgttct gaccttttga tttaaatcct
     6721 gggtgcggtc cagactggcc gcgtcgggca gtaaagcgac gccgaggagg tagccctggt
     6781 cttccgaggg cacaaagctg gtggggacat gtctaaagag aacgaagacg caggtgagca
     6841 taacggcaaa gatgcacagg gccactgcca cacgcttgat gatgagcgag acgcccctgg
     6901 agtatccttc cgtcagaccg gcgaatttcg catcgaacgt cctgaagaac cggtttttct
     6961 ccccgtgccg gggtttcagg agcagagcgg ccatggcggg ggaaagcgtc agggcgacaa
     7021 ttccggaaaa aaccacggac atggcgatgg taatggcaaa ctgcttgtag agctgccccg
     7081 taatgccgcc gagaaaagcg acggggatga aaacggcgca cagaacgagg acgatggcga
     7141 cgacgggccc cgtgacctcc tccatggctt tcttggccgc gtccttgggc gatagattga
     7201 attccctcat gttcctctcg acattttcga tgacgacgat ggcgtcatcg acgacaatgc
     7261 cgatggccag caccattccg aaaagcgtca gcatgttgat ggaaaatcca aagagcgtca
     7321 tgccgataaa ggttccgata atcgagacgg ggatggccag aatggggatg accgtggcgc
     7381 ggatactctg gagaaacagg aagacgacca cgatgacgag caggcaggcc tcgaagaaag
     7441 tgatgacaac ctctttgatg gaggcccgca caaatttcgt ggtgtccatg gcgattttgt
     7501 aatcaatgcc gtcgggaaag cctttcttca gctccttcag ggttttttcc acctgtttag
     7561 aaacgtccag ggagttggcg ccggcctgct gaaaaacggc aataatcgtt gcgtcttttc
     7621 catttaaacg gcagcgggtg ccatagtctt tggccccaaa ttccgcccgc ccgacgtctt
     7681 tgaggcgaac catggcggcg ccttgagcgt ttacccggag gatgatattt tcaaattcgg
     7741 cgggttcgat gagccttccc ttggtcgtca cggagaaggt ctgctctacg cgccccttcg
     7801 tcggggcctg gccgatcttg ccgacggcga actgctgatt ctggtttttg atggcattgg
     7861 ccacgtcgga ggctgtgatc cccagctgaa tgagccggtc gggcttcagc cagatgcgca
     7921 tggcatagtc gggcatgccc atgatcgccg cctgactggc gccggggatt cgcttgatgg
     7981 cgtcgagaat atacagattg gcataattgg cgacatactg gtggtcatag cggtcgttgg
     8041 gcgaatagat gccgacgacc ataaggaagc tgctcgattt cttgctgacg atgacgccct
     8101 gttgctggac ggattgggga agctgaggca aggcctgatt gacgcggttt tgaatgtcca
     8161 cctgggccag atctgggctg gtgccgattt cgaaatagac gctgaggttc atcatacccg
     8221 agctggaact tgtggaggac atatagatca tattatccgc cccgttcact tgaagttcga
     8281 tgggggcggc gatattttcg gagatgacgt cggcgctggc gcccggatag gttgccgaga
     8341 cggtgaccac gggaggtgtg atatccgggt attgggcgat gggcagattg ctcatggcca
     8401 ccagcccggc gataacaata acgatggaaa tcacggaggc aaaaacaggg cggtcgatga
     8461 agaagtggga aatcatttat aattttctcc tatttcttca ccggcggcgc cggcgatttc
     8521 tgatccgtga caggtgcggc gcctttggca accggggacg tatcgatctt gaggggtgct
     8581 cccggcgaca tcttgagcag gttgtcgagg atgatgcggt cgccggcggc aaggccgctg
     8641 ttgatgaaca catcattgcc ctgccagtct ccgacttcca cggcgcggaa ttccgccttg
     8701 ccgtctttcc ccgcgatcca gacgaaaaat ccctgcgcgc cctggttgac ggccgttcgg
     8761 gggatcagga tgccgttggg gcggatgcct cccagcaggt gcacgcggac gaactgcccg
     8821 ggccggatga ccgtcgggga gttgatggga ttgggaattt ccgcccgaac ctcataggtt
     8881 ccggtttcgg aattatagga aggatcggcg aaattaaggc ggcccttgtg ggggaagatc
     8941 gagccgtcgg ccatttgaac cttgacctcg aaatcggagc ccttgggcat tttaaaaagc
     9001 cctttggcca gatggtccat cagcttgagg ttttcgtttt ccgagatgct gaaatccacc
     9061 cagatggggt cgatcttggc cacataggtc agaaggctgg cctgacccac ggagatgtag
     9121 cttccctcct gctgctttgc ccgtccggcc aatccgtcaa tgggggaggt aatcgtggtg
     9181 tagctcagtt tcagctccgc gtcctggacg cttccctggg cggcaaagac ggcggcgcgg
     9241 gcgctctttt ccgcgctgac ggcgtcatcg aggtctttct ggctgacggc gtttttctcc
     9301 gcgaggggac ggactctttt cagcgtggct tcggcgttga ccagcttggc ttcctgctgg
     9361 gccagcgcgc cacgggcctg ctggagagaa gcttcgaacg gccggcggtc gatctggaac
     9421 agcgtttgac cggaccggac gcgtccgcct tcctcatagg ttttcttttc cagaaagcct
     9481 tcgacgcggg cgcggatttc cacctgatgg gagctttccg tcgtgccgac aaattcgatg
     9541 tcaatgggga catcccgggg agcgaccgtc atgaccgtga cgggaatggc cggccgggaa
     9601 tgctctttgt cgcttttatc gccgcatccc gaaaaggcgg ccgcgaagat gagaaaccag
     9661 ccgatgcaaa ataccaggaa aaaaaacgag cggtgcgaac gacctgtacc atccatgtac
     9721 aaacctccat ccgtgtaaaa aagctgcgtg acgaatgttt tgaaaaattc agaatgactt
     9781 tttcatcgga gccatccggt tggaaccgga atccctggcg atcggacgat tccggatgca
     9841 ttcttcgacg agatgccccc atcggggcgt tttgcgaaac tctcctgtga acgccaattg
     9901 ttgtcctggc tgcaacatat aggaaaattc caccgccaag tcaatggttt tatatgcctt
     9961 atgtctgaac ctttggcgga cgtcttttgg gttcatccgc tctgttattc acacggatgc
    10021 ccgacggggt cgaaggcgtc cttaggtggc aagacccggc agcgcgatcc gtttctccag
    10081 tctgcgcgct gcaagaacga tgatggaaat gagcgccagg taaactgctc cggccgtgac
    10141 ataggccttg aaaaactgaa aattggttga ggcaacctcc tgcatctggg caaaaaagtc
    10201 atgatactga ataagaaaag cgacggaagt gtctttcagg ttctggatcg cctggttggt
    10261 cagcgaagga acggaca
//
LOCUS       DAOPAF010000056        10266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6330_length_10266_cov_50.743708, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000056 DAOPAF010000000
VERSION     DAOPAF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_10266_cov_50.743708"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLQ77_06150"
     CDS             <1..220
                     /locus_tag="PLQ77_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPL10292.1"
                     /translation="DLLAALDRAVPLSANYRHTEGNSAAHVKSSMVGASEMVIIENGR
                     LVLGTWQSIFFCEFDGPRSRKVIVKFLT"
     gene            complement(367..1170)
                     /locus_tag="PLQ77_06155"
     CDS             complement(367..1170)
                     /locus_tag="PLQ77_06155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPL10293.1"
                     /translation="MRELTDFAVYLARKAGRMLAEKFTRHNPVYYKGAIDLVTEADRM
                     SEEIILAEIGRRYPGHGILSEESKEKNARAAMRWIVDPLDGTTNYAHGFPFFCVSVAL
                     EKDGSVVLGVVYDPLRDDLFEASLGGGSRLNGKELRVSSVEDLSRSLLATGFPYDIRE
                     SPDNNLNFFNAMAKKAQAIRRAGAAALDLAYLAAGRFDGFWELKLKPWDTAAGCLLVE
                     EAGGVLSDMAGEKWHLFSPGLVASNGLIQEQMLEVMSAVVNGGKTGEGQ"
     gene            1310..2206
                     /locus_tag="PLQ77_06160"
     CDS             1310..2206
                     /locus_tag="PLQ77_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPL10294.1"
                     /translation="MTTEKNADSPIPDLKTARQNSGQTLKEMFERTRISVVNLEAIEN
                     GQFHLLPVPTYTRNFIKIYAQALGVDSSPLLGRYEEYLQALKNKEKEQAAVKPPPAPL
                     AEMLSRYKIFLWITLIVILIAAVSIFASLYNKPLSESQQGIQRTGEAEGRAAPAQVAA
                     GADALPVGIGLPKPEEIAGVQPPESQQVMASPSIPSSAVSQTPAAQKAPEKDKAAQAA
                     AGAEETSLLIIRATEQTWIRMQVDDKEPYEVLLEAGETLSQKASRFNMDVGNAGGIRI
                     QLDGKTIENLGKSGQVIHLRLP"
     gene            2248..2412
                     /gene="tatA"
                     /locus_tag="PLQ77_06165"
     CDS             2248..2412
                     /gene="tatA"
                     /locus_tag="PLQ77_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPL10295.1"
                     /translation="MPELLIILVIILIIFGVGKLPEIGRGLGEGIRNFKKSVSDKNDA
                     NPPAKKEGDK"
     gene            complement(2454..2861)
                     /locus_tag="PLQ77_06170"
     CDS             complement(2454..2861)
                     /locus_tag="PLQ77_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPL10296.1"
                     /translation="MVNKALLIGRLGRDPEVRYTPDGTMVTNFTMATDETYKDKNGER
                     VQKTEWHRIVAFGKLAEICGNYLVKGKLVFIEGRIQTRAWEDKEGVKRNTTEIIASNM
                     QMLDSKGQAKSGDATPEAPSAQNSGTPMDDVPF"
     gene            3032..4513
                     /gene="lysS"
                     /locus_tag="PLQ77_06175"
     CDS             3032..4513
                     /gene="lysS"
                     /locus_tag="PLQ77_06175"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPL10297.1"
                     /translation="MEDNDLLKVRLEKIAALKAAGTDLYPNDAKPQNTTDRILQEYGG
                     MDSDGLAKLTQKFSLAGRLMAIRSFGKAAFVKIQDRKGQLQCYIAKNILSEQDFSIFK
                     KLDIGDIIYVSGKLFRTKTNELTLEAESVRLTAKSIRPLPEKWHGLTDIETRYRQRHL
                     DLISNPAVKEIFIRRSLIIRLIRQFMDRHDFLEVETPMMQPKAGGAVARPFKTHHNAL
                     DQDLYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYASYATYE
                     DLMSFTEELFAFIAREIFGKLAFTYQGTEIDLTPPWRRLAVKDALVEIAGMTPDEMAD
                     PAKAIDFARNAGCDIKETDPPGKILMAIFDERVEKKLIQPTFVTHYPVAVSPLSRRSN
                     TDPDLVDRFELYISGREIANAFSELNDPADQRERFAAQLKEREAGDDEAHEMDEDYIR
                     TLEYGMPPTAGEGIGIDRLIMLLTDSASIRDVILFPLLRSRTE"
     gene            4575..5861
                     /locus_tag="PLQ77_06180"
     CDS             4575..5861
                     /locus_tag="PLQ77_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HPL10298.1"
                     /translation="MSYEFFISRRYMRAKRKQVFVSIITFISIFGIFLGVAALIIVLA
                     VMNGFEEDLRSKILGIRSHIEVTADMSGPMKNYPAVRREIEQIPGVVASTPFIYTEAM
                     IQSPNGVTGVVIRGMETQSAPRVINLGKIRQGSVDHLDGIPEAVRQKLPAADRELPGI
                     LIGREMARNMGIFLHDPITIISPAGGIATPMGMVPRMKKFVVTGIYESGFYEYDSKLA
                     FLSLASCQDFLEMGDAVSGIDIVVRDIYQADVIARKIQSRLGFPFYAQNWMQMNKNLF
                     SALRLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKDIAILKSMGATSATIMKIFI
                     YQGLIVGMVGTFFGSVAGLAIALNLQKISLLVEKVFNFKILPGDVYYLSELPSKVNYG
                     DVALIVAGTLAICFLSTIYPSWRASRLDPAEALRYE"
     gene            5883..6551
                     /locus_tag="PLQ77_06185"
     CDS             5883..6551
                     /locus_tag="PLQ77_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10299.1"
                     /translation="MIRLENLSKTFVKDGNKIEVLKGLNLEIARGDSLAVVGVSGAGK
                     STLIHILGTLDHPTEGLLSIAGQAVFDWNERKIAAFRNRTIGFVFQFNNLLPEFTALE
                     NAMMPALIGGLNKKEANDRAARLLTEVGLEHRLQHKPGELSGGEQQRVAIARALVMEP
                     EILLADEPTGNLDTETGKKIEDILIDLNAEKKITLVVVTHNRLLAERMSGRIGLRDGK
                     IYDF"
     gene            6541..9186
                     /gene="bamA"
                     /locus_tag="PLQ77_06190"
     CDS             6541..9186
                     /gene="bamA"
                     /locus_tag="PLQ77_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPL10300.1"
                     /translation="MIFNFKTVRLFIAVVLLLFAMEARADAPKKICILPFEVHASTGS
                     AELQASVHNSLLTEFRREKKVEVIAAGAFEPGGPAPGEAEAGAAGKELGADYVVFGSV
                     MRFGETLNIDAQIIDVAGEKILSAVSVQGKETEGAGALAGALKREILGRAGLTDKIAR
                     VEIAGNRKIGADAVVQKLKSRPGKVLDEDDIRDDIKTIFKMGYFLDVSADVTAEAGGK
                     VLTFNVVEKGLISEIRLIGNKKLDRDDILEAMSVKVRQSVNPENVKQDLQKIKALYDA
                     KGYYNAEISDRLEKDGEKDFVLILDIRENDRVYIRSITFEGNESFTAKELAGIMTTNE
                     RTLLGFITDTGILQPQQLKQDVQRLTAFYFNNGFVNAQVGEPEIRHDDKGIYIHFTVR
                     EGKRFKMGKVEISGDYLEKTRDDLYASLKTKAGSHYSREAIMKDIEMITTTANDEGYA
                     NADVYPQVATHEKEQLVDLNFVVKKGELIHIARIGISGNTTTRDKVIRRSLFIVEGDL
                     YSSTKLKKSYENLNQLRYFEEVDIQTEKAPDNQMDINIRVKEKNTGMFMIGAGYSAVD
                     QAVIMAQIVQQNFLGYGQTMSLKASLGSTTNNYELSFTEPYLFDLPLWFKADVWKYKK
                     EYDSYSLDTKGVGVTVGHPLFWKIVGYVGYKFSMDNIQDINAATASTYIKAQAGERTS
                     SALSFTMVYDTTNDVMFPSKGIKASASVQHAGMPLGGNTNFTKYATTATGFVPLFWDM
                     VFSLKGQIGYLQNNDDSDDKLPIYERYVLGGINSLRGLRYVGPTNPGTSDVIGGTTML
                     TMTAEIVFPLIKNAGMKGVVFYDTGNTWNGGYHLNDLRSTCGAGIRWYSPIGPLRLEY
                     GYVLDNTGLNDDSVGRWEFTIGMFM"
     gene            9253..9783
                     /locus_tag="PLQ77_06195"
     CDS             9253..9783
                     /locus_tag="PLQ77_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPL10301.1"
                     /translation="MKKNMLWIAGLMLLLFAWTNTSLAADRIGFINMQTIIQNSNAGK
                     KAAEDFKKLFARKQESIKTMENEVKKLKDELDKQGAVMTAGVRGDKEAAYQRKMRDYQ
                     ILVDDTNKELQKRDQEYSQQLIPEILKVVRTIAEKEKYTLILDVSTMPLPYFDKAGDI
                     SRKVIEDFNKTQVGKK"
     gene            9798..>10266
                     /locus_tag="PLQ77_06200"
     CDS             9798..>10266
                     /locus_tag="PLQ77_06200"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HPL10302.1"
                     /translation="MKKSIAEIAALVGGTVTGDDQKWISDIRPIEEAGADDLSFVANP
                     KYFKWLKTTLAGAILVPPGTVAPDKNLIAVADPYAAFGKLLTVFYPVDHGPAGISPDA
                     FIEEGAFVSPEATVMPRAFIGRGARVEKGAVIYPGVFIGRDASVGEDSVLYANV"
BASE COUNT         2521 a         2890 c         2804 g         2051 t
ORIGIN      
        1 cgatcttctg gcggcgctgg accgggccgt tcccctgtcc gcgaattacc ggcacacgga
       61 gggcaattcc gctgcgcacg tcaaatcgtc gatggtcggc gcgtccgaaa tggtcatcat
      121 cgaaaacggc cgcctggtgc tgggcacctg gcaatccatc ttcttttgcg aattcgacgg
      181 cccgcgatcc cgaaaagtta tcgtcaaatt tctcacgtga ttagtgaatg gtgtatggtg
      241 tatcgctctt agccaccatc cgctatccac tataaaccaa agcggggctg aagccccgcc
      301 ctacatttca ctcattgatc gtcactttta actacaagcc acggtctttt acctatcgcc
      361 cattgcctat tgcccctcgc ctgtttttcc accattcacc actgctgaca tgacctccag
      421 catctgctcc tgaatcagtc cgttgctggc gacaaggccc ggagaaaaaa gatgccattt
      481 ttcgccggcc atatcggaaa ggacgccgcc cgcttcttcc accagcaggc atccggcggc
      541 cgtgtcccag ggtttgagct tgagctccca gaagccgtca aaccgtccgg cggcaagata
      601 ggccaggtcc agggccgccg cgcccgcgcg ccggatcgcc tgcgcttttt tggccattgc
      661 gttgaaaaag ttcaggttgt tgtccggact ttcacggatg tcatagggaa agccggtggc
      721 cagaaggctg cgggacagat cttccacgga agaaacgcga agctccttcc cgttgaggcg
      781 cgacccgcct cccagcgaag cctcaaacag gtcgtcccga agcggatcat aaacgacgcc
      841 caaaacaacg ctgccgtctt tctccagggc aacggagacg cagaaaaagg gaaagccgtg
      901 ggcgtaattc gttgttccgt ccagaggatc cacaatccag cgcatcgcgg cccgcgcgtt
      961 tttctccttt gattcttcgg ataaaatacc atgaccagga taccggcggc cgatttccgc
     1021 aaggatgatc tcctccgaca ttcggtccgc ttccgtcacc agatcgatgg ctcccttgta
     1081 gtaaaccgga ttgtgccggg tgaatttttc agcaagcatt cttcccgcct ttcgcgccag
     1141 gtaaacagca aaatccgtta gttccctcat gaccggccat tcctccatat catcagcaat
     1201 caacggtgtt taaaccacaa agccggcaaa aaaggtagag cattcttaaa cattgcgctg
     1261 gtgaatattt aatgctaaac agaccaaaaa tgttgttggt ttgcggttca tgacgacaga
     1321 aaaaaatgcg gattccccca tccctgattt gaaaacggcg cggcaaaatt caggacagac
     1381 cctgaaggaa atgtttgagc gcacacggat cagtgtggtc aatctggaag ccatcgaaaa
     1441 cggtcagttc catctgctgc ccgtcccgac ctacacgcga aatttcatca aaatatacgc
     1501 gcaggccctg ggggtggaca gctccccgct gctcgggcgc tacgaagaat atctgcaggc
     1561 gctgaaaaac aaagaaaagg aacaggccgc cgtaaagccg ccccccgcgc ccctggccga
     1621 aatgctgagc cgttacaaaa tcttcctgtg gatcaccttg atcgtcattc tcattgcggc
     1681 cgtgtccatt ttcgcgagcc tttacaataa acctttgtcc gaatcgcagc agggaatcca
     1741 gcgaaccggg gaagcggaag gccgggcagc cccggcgcag gtcgcagcgg gggctgacgc
     1801 gctccccgtc gggatcggtt tgccgaagcc ggaggaaatc gcgggagtcc aaccgccgga
     1861 gagtcaacag gtcatggcgt cgccgtccat tccgtcctcc gccgtttcac aaactcccgc
     1921 cgcacagaag gcgccggaaa aggacaaagc cgctcaagcg gcggcaggcg cggaagaaac
     1981 gtctcttttg atcatccgcg ccacggagca aacctggatt cgaatgcagg ttgacgacaa
     2041 ggaaccttac gaagttttgc tggaagcggg agaaacgctc tcgcaaaaag cgtcccgctt
     2101 caacatggac gtcggcaatg ccggcggaat ccgaatccag ttggatggaa aaaccataga
     2161 gaatttaggc aagtcgggtc aagtgatcca cttgcgctta ccctgaagcc attaaagaaa
     2221 agagggagtt ttatgtttgg aataggcatg ccggagttgc tgatcatcct ggtcatcatt
     2281 ttgattattt tcggcgtcgg aaagctgccg gaaatcggca gggggctcgg cgagggaatc
     2341 cggaatttca agaagtccgt ctccgacaag aatgatgcga acccgccggc aaaaaaagag
     2401 ggagacaagt aattctgcct ccccgttttg gctcatgaat tcattcgtga aggctagaac
     2461 ggaacgtcgt ccatcggcgt gccgctgttt tgcgcggagg gcgcctcggg cgttgcatcg
     2521 ccggatttgg cctgcccctt ggaatccagc atttgcatat tgctggcaat aatttccgtt
     2581 gtgttgcgtt tcacgccttc cttgtcttcc caggccctgg tctgaatccg tccttcaatg
     2641 aaaaccagct ttcccttgac caagtaattg ccacagattt ccgccagttt gccgaacgca
     2701 acgatccggt gccactcggt tttctgtacc ctctccccgt ttttatcttt gtaggtttca
     2761 tccgtcgcca tggtgaagtt cgtcaccatc gtgccatccg gcgtgtaacg gacctccgga
     2821 tctctgccca gtctgccgat caatagcgcc ttgttgacca tgattgcctc cccgtgaaat
     2881 gagttggcgg catcatacat aacaaccccc tgggaagcaa acacattttt tgattttaaa
     2941 gcccttgggc caatgggttg cggccagaat cgggcttgac taatccccaa aaaaaatata
     3001 gtcaaacaga cttttaaaaa gcggagaaat tatggaagac aatgatcttt taaaggttcg
     3061 tctggaaaaa attgcggcat tgaaagcagc cgggacggac ctgtacccca acgacgcaaa
     3121 acctcaaaat acaaccgacc ggatccttca ggaatacggc ggtatggaca gcgatggtct
     3181 ggcaaagctc acacagaaat tttcgctcgc cggacgcctg atggccatcc gcagcttcgg
     3241 caaggcggct tttgtcaaaa tccaggaccg gaaaggccaa ctgcagtgct acattgccaa
     3301 aaacatcctc agcgagcagg atttttccat ctttaaaaag ctggacattg gcgatattat
     3361 ttatgtttcc ggaaagcttt tccggaccaa gaccaacgag ctgacgctgg aggccgagtc
     3421 ggtgcgcctg acggccaaat ccattcgccc cctgcctgaa aagtggcacg gtctgacgga
     3481 catcgaaacc cgctaccgcc agcggcatct ggatctgatc tccaacccgg cggtgaagga
     3541 aatcttcatc cggcgcagcc tgatcatcag gctgatccgg cagttcatgg accggcacga
     3601 ttttctggaa gtggaaaccc ccatgatgca gccgaaagcc ggcggcgccg tcgcccggcc
     3661 gttcaaaaca catcacaacg cgctggatca ggatttgtat ctgcggatcg cccccgaact
     3721 ttatctgaag cgcctggtca ccggcggcct ggaaagagtg tatgaaatca accgcaactt
     3781 ccgcaacgaa ggcatctcca cgtttcacaa cccggaattt acgatgatgg agttttacgc
     3841 aagctacgcg acctatgaag atttaatgtc cttcacggaa gagttgttcg ccttcatcgc
     3901 ccgggaaatc ttcggaaaac ttgcatttac ctaccagggc acggagattg atctgacgcc
     3961 gccgtggcgt cgtctggccg tgaaagacgc gcttgtggag atcgccggca tgacgcccga
     4021 cgaaatggcc gatccggcga aagccattga ttttgcgcgc aatgccgggt gcgacatcaa
     4081 ggaaacggac ccgccgggga aaattctgat ggcgatcttc gatgaacggg tcgaaaaaaa
     4141 gctgatccag ccgaccttcg tcactcatta tccggtcgcc gtttcgccgc tttcccgaag
     4201 gagcaacacc gacccggacc tggtggaccg tttcgagctt tacatttccg gacgggagat
     4261 cgccaacgcc ttttccgaac tgaacgatcc ggccgatcag cgggaaaggt ttgccgcgca
     4321 gctcaaagag cgggaagcgg gcgacgacga agcccacgaa atggatgaag actacatccg
     4381 aacactggag tacggcatgc ccccgacggc aggagaaggg atcggcatcg accgcctgat
     4441 catgcttctc accgattccg cgtccattcg ggacgtgatt ttatttccgc ttctgaggtc
     4501 caggacggaa tgacgaaccc gcggtttttc gcgcggcggc ccgaatcaac ggcttaccca
     4561 caggacagag cttgatgtct tacgaatttt tcatcagcag acggtacatg agggcgaaac
     4621 gcaagcaggt ttttgtttcc atcattacct tcatatcgat tttcggcatt ttcctgggcg
     4681 tggccgcgct catcatcgtg ctggccgtca tgaacggctt tgaagaagat ctgcgcagca
     4741 agattctggg catccgctcc cacatcgagg tgacggcgga catgagcggc ccgatgaaaa
     4801 attacccggc ggtccgccgc gaaatcgaac aaatcccggg agtggtcgct tccacgccgt
     4861 tcatttacac ggaagccatg atccaaagcc caaacggcgt caccggcgtg gtcatccgcg
     4921 gcatggagac ccaaagcgcg ccccgggtca tcaacctggg aaaaatccgc cagggcagcg
     4981 tcgatcatct cgacgggatc cccgaagccg tccgtcaaaa actgcccgcg gccgaccggg
     5041 agctgccggg cattctgatc ggcagggaaa tggccagaaa catggggatt tttctgcacg
     5101 atccgatcac tatcatctct ccggcgggcg gaatcgccac gccgatgggc atggttcccc
     5161 gcatgaagaa attcgtcgtg accggaatct acgaatcggg attctacgaa tacgattcca
     5221 agctggcttt cctgtcgctg gcgagctgcc aggattttct ggaaatgggc gacgccgtct
     5281 cgggcattga cattgtcgtg cgggacatct accaggcgga cgtcatcgcc cgcaaaatcc
     5341 aaagccggct gggcttcccc ttttacgccc agaactggat gcagatgaac aaaaacctgt
     5401 tttcggccct caggctggaa aagcgagtca tgttcattat cctgagcctg atcgtgctgg
     5461 tggccgcctt caacatcatc agcgcgctga tcatgatcgt catggaaaag aacaaagaca
     5521 tcgccatcct gaaatccatg ggcgccacct ccgcaaccat tatgaaaatc ttcatctatc
     5581 agggattgat tgtcggaatg gtgggaactt ttttcggctc tgtcgccggc cttgccattg
     5641 cgctgaatct gcagaaaatc tctcttcttg tggagaaagt tttcaatttc aaaattctgc
     5701 cgggcgacgt gtattactta agcgaactgc cgtcgaaagt gaattacggc gacgtggccc
     5761 tgatcgtcgc gggcacgctc gcaatctgtt ttctgtctac gatttatcca tcctggaggg
     5821 cgtcgcggct ggacccggcg gaagcccttc ggtacgaata acgcgagggt cttgcaaagc
     5881 atatgatcag actggaaaat ttatccaaaa ccttcgtgaa ggacggcaac aagattgaag
     5941 ttctgaaagg gctgaacctg gaaatcgcca gaggcgactc cctggccgtc gtcggcgtgt
     6001 ccggcgcggg gaaaagcacg ctgatccata tcctcggcac gctggaccat ccgacggaag
     6061 ggttgctttc cattgccgga caggcggttt ttgactggaa tgaaaggaaa atcgcggcct
     6121 tccgcaatcg aaccatcggc tttgtgtttc agttcaacaa ccttctgccg gagttcaccg
     6181 cactggaaaa cgccatgatg ccggcgctga tcggtggact gaacaaaaaa gaggccaatg
     6241 accgggccgc gcgtcttttg acggaggtcg gtctggagca tcgccttcaa cacaaaccgg
     6301 gggagctctc cggaggagaa cagcagcgcg tggccatcgc ccgcgccctg gtgatggagc
     6361 cggaaattct gctggcggac gaacctacag gaaaccttga cacggaaacc ggaaaaaaga
     6421 tagaagacat cttaattgac ttgaatgccg aaaaaaaaat aacgctggtt gttgtcacgc
     6481 acaacagact gctggcggaa aggatgtccg gcagaatcgg cctgcgcgac gggaagattt
     6541 atgattttta atttcaaaac ggtccggctc ttcatcgccg ttgtgcttct tttgtttgcg
     6601 atggaagccc gagcggacgc cccaaaaaaa atatgcattc tgccctttga ggttcacgcg
     6661 agcaccggca gcgccgagct gcaggcgtcg gtgcacaaca gcctgctcac ggaattccgg
     6721 cgggaaaaga aagtggaagt gatcgccgcc ggcgctttcg aaccgggcgg ccccgcgccg
     6781 ggcgaggcgg aagccggtgc ggcgggaaaa gaactggggg cggactacgt cgtattcggc
     6841 agcgttatgc ggttcggtga aaccctgaac atcgacgcgc aaattatcga tgtcgccgga
     6901 gaaaaaatcc tgtccgccgt ttcggttcag ggaaaggaaa cggagggcgc cggggcgctg
     6961 gccggagccc tgaagcggga aattctgggc cgggcggggc tcacggacaa aatcgcgcgc
     7021 gtggagatcg cgggcaaccg caagatcggc gcggacgccg tcgtgcagaa gctcaaaagc
     7081 aggcccggca aggtcctcga cgaagacgac atccgcgacg acatcaaaac catcttcaaa
     7141 atggggtatt ttctggacgt cagcgccgac gtcacggcgg aggccggggg aaaagtgctg
     7201 accttcaacg tggtcgaaaa aggcctgatt tcggaaatcc ggctgatcgg caacaagaag
     7261 ctggaccgcg acgacatcct ggaagccatg agcgtcaaag tgcggcaaag cgtcaatccg
     7321 gaaaatgtca agcaggacct gcagaaaatc aaggcgctgt acgacgccaa gggctactac
     7381 aacgccgaaa tctccgaccg cctggaaaaa gacggcgaga aggatttcgt tctgatcctg
     7441 gatatcaggg aaaacgaccg ggtctatatc cgctccatca cgtttgaggg gaacgaatcc
     7501 tttaccgcca aggaactggc cggcattatg accacaaacg agcgaaccct tctgggcttc
     7561 ataacggaca cgggcatcct gcagccccag cagctcaagc aggacgttca gcgtctgacc
     7621 gctttttatt tcaacaacgg cttcgtgaac gcccaggtcg gggagccgga aatccggcac
     7681 gacgacaagg gcatctatat ccattttacc gtccgtgaag gaaagcgctt caaaatgggc
     7741 aaggtggaaa tctcggggga ttacctggaa aaaaccaggg atgacctgta cgcttcctta
     7801 aaaaccaaag ccggaagcca ctacagccgg gaagcgatca tgaaggacat cgaaatgatc
     7861 acgacgacgg ccaacgacga ggggtatgcc aacgccgacg tctatcccca ggtcgccacc
     7921 catgaaaagg agcagcttgt ggatttgaac ttcgtcgtga agaaaggcga actcatccac
     7981 atcgcccgca tcggcatttc cggcaacacc accacccgcg acaaagtgat ccgccgttcg
     8041 ctgttcatcg tggaggggga tttgtattcc tccaccaagt tgaaaaaaag ctacgaaaac
     8101 ctcaaccagc ttcgctattt cgaagaagtg gacatccaga cggaaaaggc gccggacaac
     8161 caaatggaca tcaacatccg ggtcaaggaa aaaaacaccg gcatgttcat gatcggcgcg
     8221 gggtacagcg ccgtggacca ggcggtgatc atggcgcaga tcgttcagca gaattttctg
     8281 ggctacgggc agaccatgag cctgaaggcc tcgctcggtt ccacaaccaa caactatgaa
     8341 ctgtcgttta ccgagccgta cctgtttgac ctgccgctgt ggttcaaggc cgacgtgtgg
     8401 aaatacaaaa aggaatacga ttcctactcg ctggacacga aaggcgtggg ggtgaccgtg
     8461 ggccacccgc tgttctggaa aatcgtgggc tacgtcggct acaagttcag catggacaac
     8521 atccaggaca tcaatgccgc tactgcgtcc acctacatca aggcgcaggc gggcgaacgg
     8581 acgagcagcg cgctttcctt cacgatggtc tatgacacca ccaacgacgt catgttcccc
     8641 agcaagggca tcaaggccag cgcgtccgtg cagcatgccg gcatgccgct gggcggaaac
     8701 accaatttca ccaagtacgc caccaccgcg accggctttg tcccgctctt ctgggacatg
     8761 gtcttctccc tcaagggcca gatcgggtat ctgcagaaca atgacgacag cgacgacaag
     8821 cttccgatct acgaacggta tgtgctgggc ggcatcaact ccctgcgcgg cctgcgctac
     8881 gtcgggccga ccaatccggg aaccagcgac gtgatcggcg gcaccaccat gctgaccatg
     8941 accgcggaaa ttgtttttcc gctcatcaaa aacgcgggta tgaagggcgt cgtgttttac
     9001 gacacgggca acacgtggaa cggaggctat cacttaaatg acctgagaag cacctgcggc
     9061 gcgggcatcc gctggtattc ccccatcggc ccgcttcgcc tggaatacgg ctacgtgctt
     9121 gacaacacgg gcctgaacga tgacagtgtg ggccgctggg aatttaccat cggcatgttc
     9181 atgtaaaccg gagccaggac gccaaacgtc agggttcaat cataaaccaa tcaaccgaga
     9241 ggagattttg aaatgaaaaa aaacatgctg tggatcgcag gactgatgct gcttcttttt
     9301 gcctggacaa acacgtcgct ggccgccgac cggataggct tcatcaatat gcagacgatc
     9361 attcagaatt ccaacgcagg caaaaaagcg gccgaagact ttaaaaagct ttttgcccga
     9421 aagcaggaaa gcatcaagac catggaaaac gaagtcaaga aactcaagga cgaactggat
     9481 aagcagggcg ccgtgatgac cgccggcgtg cgcggcgaca aggaagccgc ctaccagcgc
     9541 aagatgcgcg actaccagat cctggtcgac gacaccaaca aggagctcca gaagcgcgac
     9601 caggaatatt cgcagcagtt gatccccgaa atcctgaaag tggtccggac catagccgaa
     9661 aaggaaaaat acaccctgat tctggacgtg agcaccatgc cgctgcccta tttcgacaag
     9721 gccggcgaca tcagcaggaa agtcattgaa gacttcaaca agacgcaggt cggcaaaaaa
     9781 taattttgtc gcgaaatatg aaaaaaagca ttgcggaaat agccgccctg gtgggcggca
     9841 cggtcacggg cgatgatcaa aagtggatta gcgatattcg gccgatcgag gaggcgggcg
     9901 cggacgatct ttcgtttgtc gcaaatccaa agtattttaa atggctcaaa accacactcg
     9961 cgggagcgat cctcgtcccc ccgggaaccg tcgcgccgga taaaaacctg attgccgtag
    10021 ccgatcccta cgcggcgttc ggcaagctgc tcacggtctt ttatcccgtc gatcacggcc
    10081 ccgcgggtat aagccctgat gccttcatcg aagaaggggc tttcgtctcc ccggaagcaa
    10141 ccgtcatgcc ccgggcgttc atcggccgcg gcgcccgcgt tgaaaaaggc gcggtgattt
    10201 atcccggcgt gtttatcggc cgcgacgctt cggtcggaga agattccgtc ctgtacgcca
    10261 acgtct
//
LOCUS       DAOPAF010000057         9836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6601_length_9836_cov_54.431960, whole genome shotgun sequence.
ACCESSION   DAOPAF010000057 DAOPAF010000000
VERSION     DAOPAF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_9836_cov_54.431960"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLQ77_06205"
     CDS             complement(<1..393)
                     /locus_tag="PLQ77_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL10303.1"
                     /translation="MALIEVKDLAKAFDGKTVLKGINLELSEGQSKVVMGTSGCGKST
                     LLRCLNLLLPPTSGRIIFKGEDITRPGVDIRKVRADIGFVFQHFALYRHLTVEENVTL
                     GLRKLKKMKATEAVQKASHELERVGMIEH"
     gene            complement(406..1059)
                     /locus_tag="PLQ77_06210"
     CDS             complement(406..1059)
                     /locus_tag="PLQ77_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPL10304.1"
                     /translation="MTFGNILIILAEGAGYTVLVTLASISAGLAVGLSLAVLSTLRWK
                     AVSLFLAVYTYVFRSIPVLVLLFILFFGLPGIGIRISPFVSMVATLGLVSGAYLAEVF
                     RGAMAAVDGDEILAAEAAGLNRWEIFFYIIMPQMFRMSVPGMINEFTSVLKYTPFAYT
                     VGIPEIMKEAMSLAALTLRGLEIYLAVGILYFLIYKLFVMFFMTLEKRFRVPGLASK"
     gene            complement(1079..1828)
                     /locus_tag="PLQ77_06215"
     CDS             complement(1079..1828)
                     /locus_tag="PLQ77_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPL10305.1"
                     /translation="MKKSTILMILFVIFVMGLVGCGKKEESSLIQVAVCPTSPPNLFE
                     ENGKFQGMDLEIFEGFCKARNRQYKITSYDWQGMLGAVIGKQADVAFSAISITDKRKE
                     VMDFSKPYMDNTWNLVSLKSKNLVINDLAELKKYAIGYPRGMAYSDFIKNKLEPQGVY
                     KLSDVKMYPTYNEVLADLKNGNLDLAFLDGSVASVYKKKMDIQDSYVFTGFDMFGFAF
                     PKGSPLRDDFDKYLAELGPEKLKVIVDKWMK"
     gene            1986..2927
                     /locus_tag="PLQ77_06220"
     CDS             1986..2927
                     /locus_tag="PLQ77_06220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPL10306.1"
                     /translation="MPILYLAPLQGMTDRIYRNLFPLYFKGVDLAVAPFLSATKSIKP
                     GSLLRDYSPDQNSGISTIPQIMSSGPDDFTRLANALFDIGYGTVNWNLGCPFRMVVKK
                     GRGAGMLCRPDRIEAFLEKAVPALKPKLSIKLRLGLKDPDEILELIPVFNRFPLDELI
                     IHPRTAVQMYDGEVDLDRFGKCLDISRHTVVYNGDIDTVRTHRMLAGRFGSVDRWMIG
                     RGLLGNPFLAEEIKGRIERPYKEKVQILRAFHDHLFAEYSQILSGPAHITNKMKEFWT
                     YSGSFLKNREKIRKSINKTYRRDQYLDVVRTIFDDLL"
     gene            3046..3378
                     /locus_tag="PLQ77_06225"
     CDS             3046..3378
                     /locus_tag="PLQ77_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10307.1"
                     /translation="MLRYHYLFDRVRILRNEQEAPCFRKKKYSVSLLGLGRFTAVIDE
                     RDRKILSVLQKPLSLTEIAERGLIYGRKYHVDAWICMWEFIMIRKHVQRMVGQGRILD
                     LGDQLIAA"
     gene            3403..4737
                     /locus_tag="PLQ77_06230"
     CDS             3403..4737
                     /locus_tag="PLQ77_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPL10308.1"
                     /translation="MLEFARPRETLSVVDAAAIVVGMVLGVGIFKTPSIVAASAESEG
                     MLMLLWLAGGLASVVGALCYAELMSSCPHAGGDYHYLARAFGFPPAFLYAWARLAVIQ
                     TGAIAMVAFLAGDYASEIFRLGPLSSSLYAALFIVFLTAVNIAGIRQGKVTQMLLAAG
                     ILFGLLVVSAAGILADGPRAPVSSGGIVPGRQALGTAMIFVLLTYGGWNEASFLSAEV
                     RQGPRNPLKVLLFSIGTVTAVYLLVNVALLNSLGLSGMVSSKAVMADMMRGLLGASAG
                     AVVSLLILLAAVSTMNASIMTGARTAYALGRDFPVLGILGRWQGRRQTPVNALLVQGG
                     VALALVALGTGAHDGFVMMVEYSAPVFWLFFLMVGVSVFVLRRRYPDLPRPFRVPLYP
                     LTPLAFIAVCAFMLYASLAYTGRGSWVGVCVLLSGIPLWVWQKKRRTADGHS"
     gene            4773..5564
                     /locus_tag="PLQ77_06235"
     CDS             4773..5564
                     /locus_tag="PLQ77_06235"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPL10309.1"
                     /translation="MKRSLDKGGFGTPVDSGFRVSLLPGKPVLVFILAFCCLFLPAWS
                     PAADKTEAEYAAQRENMVARQIEDRGISDPGVLRAMRAVRRHKFVPESLRGGAYADHP
                     LPIGEGQTISQPYIVALMTEGIRPKSHMKVLEIGTGSGYQAAVLAELCQSVYSIEIVE
                     SLGKRAAKLLSGLYDNVHVRIGDGYQGWPQEAPFDAILVTCAPTKVPRPLEDQLKEGG
                     RMVIPVGESWVQELVVLTKRNGRLERQEIIPVRFVPMVDAKGRKY"
     gene            complement(5583..5858)
                     /locus_tag="PLQ77_06240"
     CDS             complement(5583..5858)
                     /locus_tag="PLQ77_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10310.1"
                     /translation="MPDYRVKISETTDGDLEYHHYLVTAKDETEARRHTMKFMERFID
                     DDNDPEIIENGYTFYNKAVVVRLESIKETTKDKFKEFLLKLHTINMG"
     gene            complement(6140..6907)
                     /locus_tag="PLQ77_06245"
     CDS             complement(6140..6907)
                     /locus_tag="PLQ77_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPL10311.1"
                     /translation="MSDTVLKEFKDGVLLLTLNRPESKNSFNKELWESFAAELDAARA
                     DDDVTVVVVTGAGKDFSAGQDLKDYAVSGKAHSYRITERAVVDFDKPLVAAAKGVAVG
                     GGATILFHTDVLYVGESLRMRLPFNSLGMAPEFASSYMLQMRIGPQLAAELLFTTEWI
                     DADKALLTGIATRKFADDELLPCAMAKAAEIAQLPLSSLLETKRCLKTAHRAGIEAAL
                     KVEREAMDRLAGGPASIEAMMAFMEKRKPDFRSLKKK"
     gene            complement(7047..7880)
                     /locus_tag="PLQ77_06250"
     CDS             complement(7047..7880)
                     /locus_tag="PLQ77_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPL10312.1"
                     /translation="MIIQVDPDKCIGCGTCRADCPTANIAIKDGKAVHGKWCLECGHC
                     LAVCPEKAVLFTGDYDPSEVLEYDDPKKFEIAPDRLLNFVKFRRSVRQYTDEPVSDAD
                     IAKILEMGRYTQTGANTQTLRYIVLTKETLREITPIALKTLAELDIRQVDKKTMRVPS
                     QYLDFQPIWANWHSFYQKKQRDSLFHGAPNALLIVSRTCNEVDGCFNAGHLELMINAL
                     GLGACLMGFGTFAFDRSPELRQKVGIQEGESVIFMMVFGHPKVKYLRTVNRRKVRYEK
                     I"
     gene            complement(8096..9295)
                     /locus_tag="PLQ77_06255"
     CDS             complement(8096..9295)
                     /locus_tag="PLQ77_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL10313.1"
                     /translation="MNHETLWTKDFITVTVINFVVFLTHFLLIVTIASYAVDEFHAST
                     DIAGLVAGIFIIGALIGRLGTGRVIEDIGSKKVLIISAFFFIITSALYFAAVNLFLLI
                     VIRLLHGIAFGAASTATGTIIAQIIPPARRGEGISYYSMGAILAVALGPFIGIFLIHH
                     ADFRMIFAFTSLLASASFAMSFLVNKPVYEKPAPEPSGAARRFQISDYLEFKAIPISI
                     IILVIGFSYSGLLTFISLYAKEIHLEKAASFYFLVYSIAVFVSRPFSGRLMDIRGANF
                     VVYPCLVLYALGMLLFSQAAQGITLLAAAVLLALGYGSFISCAQAISIRGVPPHRMGL
                     AITTFFIFVDMGFGVGPYVLGALVPLVGYRGLYVLMTFVILASIPLYYFLLGKKWSGR
                     PAQGPEP"
     gene            complement(9355..>9836)
                     /locus_tag="PLQ77_06260"
     CDS             complement(9355..>9836)
                     /locus_tag="PLQ77_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10314.1"
                     /translation="AGIAHVPEGRGIFGNLTVRENLQLACWQRKDKSKIAADLKNVFA
                     LFPRLEERSKQLGGMLSGGEQQMLAVGRALMSDAQMLLLDEPSMGLSPVLVREIFRII
                     REINQAGKTILLVEQNANMSLHIASRAYVLETGRIVLSGAGKELTGNPKVIEAYLGG"
BASE COUNT         2083 a         2736 c         2784 g         2233 t
ORIGIN      
        1 gtgttcaatc atgcccacgc gctccagctc gtgcgaggct ttttggacgg cttccgtcgc
       61 tttcattttt ttgagtttgc gcagccccag cgtgacattt tcctccaccg tcagatgccg
      121 gtacagcgca aaatgctgaa acacaaaccc gatatccgcg cggactttgc gaatgtccac
      181 gcccggacgg gtgatgtcct ctcccttgaa gatgatccgg ccggacgtcg gcggcaacag
      241 cagattcagg cagcgcagca gcgtggactt gccgcagccc gaagtgccca tgacgacttt
      301 ggactgccct tcggaaagtt ccaggttaat ccccttgagg acggtcttgc cgtcaaacgc
      361 ctttgccaga tctttcactt caatcagtgc catcagggtt cctttttact tggacgccag
      421 tccgggaacg cggaatctct tttccagcgt catgaaaaac atcacaaaca gcttgtaaat
      481 caaaaagtaa aggatcccga cagccagata aatttccagg cccctgagcg tcagggcggc
      541 caggctcatg gcctctttca tgatttcggg aatccccacc gtgtaggcaa aaggcgtgta
      601 tttcaaaacg gaggtaaact cgttgatcat gcccggaacg gacatgcgaa acatctgggg
      661 catgatgatg tagaaaaaaa tctcccagcg gttaagcccc gccgcttcgg cggccagaat
      721 ttcatcgcca tcgacggcag ccatcgcgcc ccggaacact tccgccagat aggctccgga
      781 aaccagcccc agggtggcga ccatcgatac gaacggcgaa atccgtatgc cgatccccgg
      841 caggccgaaa aaaagaataa acaggagaac gaggacggga atgctgcgga acacatacgt
      901 atagacggcc aaaaacagac tgaccgcctt ccagcgcagc gtgcttaaaa cggccagcga
      961 cagcccgacc gccagaccgg cgctgatgct ggccagcgtc accaaaaccg tgtatccggc
     1021 gccttcggcc agaatgatca ggatattccc gaaagtcatc ggtgcggcgg gcgcccttct
     1081 acttcatcca cttatcgacg atgactttca gtttttccgg ccccagttct gccagatact
     1141 tgtcgaaatc atcgcgcagc ggagatcctt tgggaaacgc aaagccaaac atgtcaaatc
     1201 cggtgaagac atagctgtcc tgaatgtcca ttttcttttt gtaaaccgag gcgaccgaac
     1261 cgtccaggaa tgccagatcc aggttgccgt ttttcaggtc ggccagcact tcgttgtacg
     1321 tcggatacat tttgacgtcg ctgagcttgt aaacgccctg cggctccagt ttgttcttga
     1381 tgaaatcgct gtaggccatg ccgcgcggat agccgatggc gtatttcttc agctcggcca
     1441 gatcgttgat gacaagattc ttgcttttga ggctcaccag attccaggtg ttgtccatgt
     1501 atggtttgga aaaatccatc acttccttgc gcttgtcggt aatggaaatc gcggaaaacg
     1561 ccacatcggc ctgcttgccg atcaccgcgc cgagcattcc ctgccagtcg taggaagtga
     1621 tcttgtactg ccggttccgt gccttgcaga aaccctcgaa aatttccagg tccatgccct
     1681 gaaacttccc gttttcctca aacaggttcg gcggcgacgt ggggcacacg gctacctgga
     1741 tgagagaaga ttcttctttc ttgccgcagc cgacgaggcc cataacgaaa attacgaaaa
     1801 gaatcattaa gatggttgat tttttcatga taagtcccct tgtgcgattc aatgcttttc
     1861 gcaccgttta taggggaagc cgcatgccct gtcaacgaaa aagaggataa atcttgacaa
     1921 aggtttggga atcgggaaaa agccatcccc gtaacaatga ttttgaccaa caaaggattt
     1981 caaaattgcc catcctgtat ctggctccgc tccagggaat gaccgaccgg atttaccgca
     2041 acctgttccc cctttatttc aagggggtgg atctggcggt cgcgcccttt ctttccgcga
     2101 caaaaagcat caagcccggc agtctgctgc gcgactattc tcccgatcaa aactcgggga
     2161 tttcgacgat accgcagatc atgagctccg gtccggatga ttttacgcgg ctggccaacg
     2221 cgctgttcga catcgggtac ggcacggtca actggaatct cggctgtccg tttcgcatgg
     2281 tggtgaagaa gggcaggggc gcggggatgc tctgccgacc ggaccggatc gaagcctttc
     2341 tcgaaaaagc agtgcccgcg ttgaaaccga aattgtccat caagctccgg ctgggcttga
     2401 aagatccgga cgaaatcctg gagctcattc ccgttttcaa ccggtttccg ctcgatgagc
     2461 tgatcatcca tccgcggaca gccgtgcaga tgtacgatgg cgaggttgac ctcgataggt
     2521 tcgggaagtg tctggatatc tcgaggcaca ctgtggtcta taacggagac atcgatacgg
     2581 tccggacgca tcggatgctt gccgggcgct tcgggtccgt ggaccgctgg atgatcggcc
     2641 ggggccttct gggcaatccg tttctcgcgg aggaaatcaa aggtcgcatc gaaaggccgt
     2701 ataaggagaa agtccaaatc ctgcgcgctt tccacgatca cctgtttgcc gaatattccc
     2761 aaatcctgtc cggccccgcg catatcacga ataaaatgaa ggaattctgg acgtattcag
     2821 ggagttttct gaaaaacagg gaaaaaatcc gcaaaagcat caacaaaact tatcgccgcg
     2881 atcagtacct ggacgttgtt cggaccatct ttgatgacct actgtgatgt ttggttgatc
     2941 tttgccggga attctgttac cggattttca tgcaaatcga atggaaaagg gagttcttcc
     3001 gggggtaggc cgcccgcgcc acgccagggg gcttgaggcc acgggatgct ccggtatcat
     3061 tacctttttg accgggtgcg gattctgcgc aacgagcagg aggcgccgtg ctttcggaag
     3121 aaaaaatatt cggtgtccct gctgggcctg ggaaggttca cggccgtcat cgacgagcgc
     3181 gaccgaaaga ttctctccgt cctgcaaaag cccctgtcgc tcacggagat tgccgaacgg
     3241 ggactcattt acggccgcaa atatcacgtc gacgcctgga tctgcatgtg ggaattcatc
     3301 atgatcagaa agcatgtgca aagaatggtc ggacagggcc ggattttgga tttgggagat
     3361 caattgatcg cggcctgacc tttcggagaa aggaatgtcc cgatgcttga attcgccaga
     3421 cccagagaga cgctttccgt ggttgatgcg gcggccattg tcgtgggcat ggtgctgggc
     3481 gtgggcattt tcaagacccc gtccattgtg gccgccagcg ccgaaagcga agggatgctg
     3541 atgctcttgt ggcttgcggg cgggctggct tcggtggtcg gcgccctgtg ctacgcggag
     3601 ctgatgtcct cctgtccgca cgccggggga gattaccact atctcgccag agcgttcggt
     3661 tttcctcccg cttttctcta cgcctgggcg cgcctggccg tcatccagac cggcgctatc
     3721 gcgatggtgg cgtttctcgc cggcgactat gcctcggaaa ttttccgtct gggtcctctg
     3781 tcctcctcgc tgtacgccgc gctgttcatt gtttttctca cggctgtcaa tatcgcgggc
     3841 atccggcagg gcaaggtgac gcagatgctg cttgccgcgg gaatcctctt cggattgctg
     3901 gtcgtgtcgg ccgcgggaat tctggctgac ggaccgcgag cgcctgtttc ctccggcgga
     3961 atcgttccgg gacggcaggc gctgggcacg gccatgatct tcgtgctgct gacttacggc
     4021 ggctggaacg aggcgtcgtt tctttccgcc gaagtgcggc aaggcccgcg caatccgctg
     4081 aaggtcctgc ttttcagcat tggaacggtt acggccgttt atctgctcgt caacgtcgct
     4141 cttttaaaca gcctgggcct gtccggcatg gtttcctcca aagcggtgat ggcggacatg
     4201 atgcgaggcc tgctgggcgc gtccgccggc gctgtcgtca gcctgctgat tctgctggcg
     4261 gccgtcagca cgatgaatgc gtccatcatg accggcgcga gaaccgctta cgcgctgggt
     4321 cgggattttc cggtgctggg gattctgggg cgatggcagg gaaggaggca aacaccggtc
     4381 aacgccctcc tcgttcaggg gggcgttgcg ttggctcttg tcgcgctggg aacgggcgcg
     4441 catgacgggt tcgtcatgat ggtggaatac tcggcccccg tgttctggct gtttttcctg
     4501 atggtcggcg tttccgtctt tgtgctgagg cgcagatacc cggatttgcc ccgccctttt
     4561 cgcgtgccgc tgtatccgtt gacgcccctg gcgtttatcg ccgtctgcgc cttcatgctg
     4621 tacgcaagcc tggcgtacac cggccgcggt tcctgggtgg gcgtatgcgt tctgctttcg
     4681 ggaattccac tttgggtttg gcagaagaaa agacgaacgg cggacggaca ttcgtaaaac
     4741 ctaaagtgat caaaccatgg agggggagaa aaatgaaaag aagtcttgat aaaggcggct
     4801 tcgggacccc ggtggattcg ggctttcgcg tttcgctcct gccgggaaaa ccggttttgg
     4861 tttttatcct ggccttctgc tgcctctttc tacccgcctg gagtccggcg gcggataaga
     4921 cggaagcaga gtacgccgct caaagagaga acatggttgc acggcaaatc gaggacaggg
     4981 gaatttccga tcccggcgtc cttcgggcca tgcgcgccgt caggcggcac aagtttgttc
     5041 ctgaaagcct gcgcggcgga gcctacgcgg accacccact gccgatcggg gaagggcaga
     5101 ccatttccca gccctacatc gttgctttga tgacggaggg gatccggccg aagtctcata
     5161 tgaaagtgct ggaaatcggg acgggatccg gctatcaggc cgcggtgctg gccgaactct
     5221 gccaaagcgt ttactcgatc gaaattgtcg aatccctggg aaaaagagcg gcaaagcttc
     5281 tctccggact gtatgacaat gttcatgtcc ggatcggcga cgggtatcag gggtggccgc
     5341 aggaggcgcc ctttgacgcg atcctcgtca cctgcgcccc gacaaaggtt ccccggccgc
     5401 tggaagatca gctcaaagag ggcggccgga tggtgattcc cgtcggagaa tcctgggtgc
     5461 aggaactggt tgtgctgacc aaaaggaacg gccgcctcga acgacaggaa atcattccgg
     5521 tccgctttgt gcccatggtg gatgcgaagg gccgaaagta ttgaagcaag atttctttcg
     5581 gctcagccca tattgatggt gtggagcttc agaagaaatt ccttgaattt gtccttcgtc
     5641 gtctctttga tggattcaag ccggacgacc acggccttgt tgtaaaacgt atacccgttt
     5701 tcgatgattt caggatcgtt gtcgtcatcg atgaagcgct ccatgaactt catggtgtgt
     5761 cttcttgcct ctgtttcatc tttggcggtc accaggtaat gatggtattc cagatccccg
     5821 tccgtcgtct cgctgatttt taccctgtag tcaggcatat aatcgttctc ctgtttttaa
     5881 tgaatgctgc cggccggtat atactccaat cactggccga aggcaactat cccgttggga
     5941 agcggatgaa aattttgccg caccggaaac gtccgttttt tcaatcccgc tttggcggga
     6001 cgagcgttga ttctccgcag cctgctgcga tatgatgacg ttcacttcat gcctcgacag
     6061 cttggcgcga ggtatttgac cacggccccg gcgaggaacg ggaatgcttg cccgccggaa
     6121 aagcgaaaac cggatccggc tatttcttct tcaggctgcg gaaatcgggc ttgcgctttt
     6181 ccatgaaagc catcatggct tcaatgctgg ccggaccgcc ggccagacgg tccatggcct
     6241 cccgctcgac cttcaatgcc gcttcgatgc ccgcccgatg agcggttttg aggcaccgct
     6301 ttgtttcaag aagcgagctc agcggcaatt gtgcaatctc cgcggccttc gccatcgcgc
     6361 agggcaaaag ctcatcatcg gcgaacttgc gggtagcgat gccggttagc agggccttgt
     6421 cggcgtcgat ccattccgtg gtgaacagca gctccgccgc gagctgcggt ccgatccgca
     6481 tctgcagcat gtagctgctg gcgaactccg gcgccattcc caggctgttg aagggcaggc
     6541 gcatccgcag gctttcaccg acgtagagga catcggtgtg gaacagtatc gtcgcgccgc
     6601 ccccgacggc aacccctttg gccgcggcga ccagcggttt gtcaaagtcc acgaccgcgc
     6661 gttccgtgat gcggtagctg tgcgccttgc cggaaacggc gtaatccttc agatcctggc
     6721 cggcggaaaa gtctttgccc gcccccgtga cgacgaccac cgtaacatcg tcatcggccc
     6781 gcgccgcatc cagctccgcg gcaaaagatt cccacaattc cttgttgaat gagtttttgc
     6841 tctccgggcg gttcaacgtc aacagcagga cgccgtcctt gaattccttt aaaaccgtgt
     6901 cgctcatttt tgatacctct tttctttgaa tgtatttttc cgggagtttt gccccggcaa
     6961 ttgcgcaagt tccggccgtc cctgttcacc aggccgcaaa gagcagcggg cgccgtcacg
     7021 cccggtgcaa gccggcgggt ctgaaatcag attttttcgt atcgcacttt tctgcggttg
     7081 acggttcgaa gatatttcac cttgggatgc ccgaagacca tcatgaaaat caccgattcc
     7141 ccttcctgaa tcccgacttt ttgccttaat tcgggcgatc ggtcaaacgc gaacgttcca
     7201 aaacccatca ggcaggcgcc cagtcccagc gcgttgatca tcagctccag atggccggcg
     7261 ttgaagcagc cgtccacctc gttgcaggtg cggctgacaa tcagaagcgc gttgggcgcg
     7321 ccatgaaaca gcgaatccct ttgctttttc tgataaaaag aatgccagtt ggcccagatg
     7381 ggctgaaaat ccaggtattg ggaagggacc cgcatcgttt ttttgtccac ctgccggatg
     7441 tccagttccg ccagggtttt cagggcgatc ggcgtgatct cccgcagcgt ttctttcgtc
     7501 agcacgatat agcgaagcgt ctgcgtgttc gcgcccgttt gcgtgtaccg gcccatttcc
     7561 agaattttgg cgatgtccgc gtcgctcacc ggttcgtcgg tatattgacg gacgcttctg
     7621 cggaatttga caaaattcag gaggcgatcc ggcgcgattt caaacttctt cggatcgtcg
     7681 tattcgagaa cttcggaagg atcgtagtcg ccggtaaaaa gaaccgcttt ctcggggcac
     7741 acggccaggc agtgtccgca ttccaggcac catttgccgt gaacggcctt tccgtctttg
     7801 atggcgatgt tggcggtggg gcagtccgcc ctgcatgtgc cgcagccgat gcatttgtcg
     7861 ggatccactt gtatgatcat gaaactctcc tgtgattccg gatgatgaag atttcttaac
     7921 gccgcttcct gtattctccg gagattttcc ttcttccgtc gaattgcttt ttgaaaaccg
     7981 cccgcaaaca gccgccggta cggcgatttc cggttggaat taatatgggg gccttgccct
     8041 tgtcaagata aaagaacggg atgaatccct ttatacaaga caggcacctg ttcaatcatg
     8101 gttccggtcc ttgtgccggt ctgccggacc atttttttcc cagcagaaaa taatacaggg
     8161 gaatggaggc aagaatcaca aaggtcatga ggacatacag gccgcggtat cccacgaggg
     8221 ggacaagcgc gcccagcacg tacggaccga cgccaaaccc catatccacg aagatgaaaa
     8281 aggtcgtaat cgcgagcccc atccggtgag gaggcacccc tctgatcgag atggcctgcg
     8341 cgcaggaaat aaaacttccg tagcccagcg cgagcaggac cgccgccgca agcagcgtga
     8401 tcccctgggc ggcctggctg aacagcagca tgcccagggc ataaaggacc aggcagggat
     8461 aaacgacaaa attggcgccc cggatatcca tcagtcgtcc ggaaaaaggc cgcgagacga
     8521 agacggcgat ggaatacacc agaaaataaa agctggcggc tttttccaga tggatttcct
     8581 tggcgtacag ggagatgaac gtcagcaggc cggaatagct gaatccgatg accaggatga
     8641 tgatggagat cggtatcgct ttgaattcca gataatcgga gatttgaaac cggcgagctg
     8701 ctccggacgg ctccggcgca ggtttttcat agaccggctt gttgaccaga aacgacatgg
     8761 caaagctggc ggacgccaga agcgaggtaa acgcaaagat catcctgaaa tccgcatgat
     8821 ggattaaaaa gatgccgata aacggaccca gggccaccgc caggatggcg cccatgctgt
     8881 aatagctgat gccttccccg cgccgggcag gcgggatgat ctgggcgatg atggtgccgg
     8941 ttgccgtgct ggccgctccg aaggcgatcc cgtgcagcag ccggatgacg atcagcagga
     9001 ataaattgac ggccgcaaaa tacagtgccg acgttatgat aaaaaagaag gcgctgatga
     9061 tgagaacttt cttgcttccg atgtcttcga tcacacgccc ggttcccagc cttccgatca
     9121 acgcgccgat gatgaagatt cccgcgacaa gccccgcaat gtccgtggat gcatggaatt
     9181 catccacggc ataagacgcg atcgtgacga tcagcagaaa atgcgtcaga aagacaacga
     9241 aattgatcac ggtgaccgtg ataaaatcct tggtccatag cgtttcatgg ttcatggtct
     9301 gtttcctgag tgcccgcccc ctgtgccgcg caacaagcgg aatccaaaga aaaattatcc
     9361 gcccagatag gcttctatta ccttcgggtt tccggtcagt tctttgccgg cgccggaaag
     9421 cacgatgcgg ccggtttcca gaacgtaggc gcgcgaggcg atgtgcaacg acatgttggc
     9481 gttttgttcg accagcagaa tcgtttttcc cgcctggttg atttcccgaa tgattctgaa
     9541 aatttctctc accagcaccg gagagaggcc catggagggt tcgtccagaa gcagcatctg
     9601 tgcatcgctc atgagcgcgc ggcccacggc cagcatctgc tgctctccgc cggacagcat
     9661 gcctcccaac tgtttgcttc gttcttccag ccgggggaac agtgcaaaaa catttttcag
     9721 atcggcggcg attttggatt tgtctttgcg ctgccagcag gccagttgaa gattttcccg
     9781 cacggtgagg ttgccgaaaa tgccgcggcc ttccggcaca tgggcgatgc cggctg
//
LOCUS       DAOPAF010000058         9780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6638_length_9780_cov_56.736350, whole genome shotgun sequence.
ACCESSION   DAOPAF010000058 DAOPAF010000000
VERSION     DAOPAF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_9780_cov_56.736350"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PLQ77_06265"
     CDS             <1..1823
                     /locus_tag="PLQ77_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPL10315.1"
                     /translation="GHINIFPDRDGTVRSEALLINFEEKLFPSFALQLTLKYLNYDVK
                     QVRPGKTVTFGRKSIPVYENNRMLISFNEGIPYYSYFDVVNKKIAPEVFKDKIVIIAL
                     SAAGLGTMQVTPVAVNVPPGTVIANVIDNIINGDHVIRPDWALTLELIMILIFGLYVA
                     LVIPHIKAGISAVVSLALLLVWMIATIYLFAAYGYWIKALYPALLLLVGYIVIVSKRY
                     LLTEKAKDRMEADGVETNKMLGLSFQGQGLLDMAFDKFRKCPVEDESVKELLYNLGLD
                     FERKRMFNKAAAVYQHIAQDGDFKDINDRIKKLTAAGETMIFGLSGAKKEATVIMENT
                     EIKPTLGRYEIVKELGRGAMGTVFLGKDPRINREVAIKTLRYEEIDSEQIDEVKKRFF
                     REAEAAGKLSHPNIVTIFDVGEDYDIAYMAMELLDGSDLAKYTRKENLLPPPEIVRIV
                     SSVASALDYAHANGIVHRDIKPANIMILTNGEVKVADFGIARVMATSKTQTGVVLGTP
                     SYMSPEQIAGKKVDGRSDLFSLGVVLYELISGEKPFNGDSIATLMYNITTTDPRAIKE
                     LSPDLPDAMAGIVEKLLARDVEARYQTGKALADDLLACLK"
     gene            1834..2628
                     /locus_tag="PLQ77_06270"
     CDS             1834..2628
                     /locus_tag="PLQ77_06270"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPL10316.1"
                     /translation="MNLTACGKTDQGLVRTNNEDNFYSDEKMGLLVVADGMGGHASGE
                     KASSLAVSVIRDYFQGQQIMIGDRDPSFSEATNKLACAIRLANQAVYEASQSSPQLKG
                     MGTTIVAVLLTGRKLSIAHIGDSRAYLVRAGTMEQLTDDHSMVNEQVKRNLISREEAD
                     LSEMKNILTKALGIQPELDADLDELTLLEGDILLLCTDGLSNMVTDDDALDMISSAAD
                     ASAACESLIEAANKNGGKDNITVVIGYAWKEKWYSALMKFMEFFRR"
     gene            2632..3333
                     /locus_tag="PLQ77_06275"
     CDS             2632..3333
                     /locus_tag="PLQ77_06275"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPL10317.1"
                     /translation="MAKILLKYKEAAVKEIPLDKESTTIGRKPDNDIVIDNQAVSGHH
                     ASITVEGDKLMLEDLGSLNGTFVNGQKISKTELFNGDVVLIGVHTLNVTSEKNRSQET
                     KGFAVRGRSMDETMVIAPDDQKKIVAAADKNLPEPLGGFMVVEGSTEKKEYELKERVS
                     SIGKEDGSAVKLKGLFAPKLAALVNRRKEGYFITPAGGKELKVNGKTIDRRCDLKDGD
                     LVEVANLKLQFYLKE"
     gene            3507..4547
                     /locus_tag="PLQ77_06280"
     CDS             3507..4547
                     /locus_tag="PLQ77_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10318.1"
                     /translation="MASHEESSFLEAVESRLDSIFGADSKPARKTDSGIPEEIGDGAA
                     DGLREDSLSPGTGKASSVLGPKEDRSAFLSEIDKRFEAIFGDETPPAREAQRASEPDD
                     LKELTARAEAEESAKEERLSEGLTLFPSLSADFPLKNLQTIVLSLEDRMNESGLERLD
                     DEVCRLDRLLESDPVLQGLLRILRFAGRYIRVKDDRFGGDAAALLFSVYRRMEQATGG
                     EEMAQEAKRRLLQESIEQYQAWVENADLEKRIEPEAPRGMTDGERARDAERRNVPMDE
                     MPVARIRVVEDKSPPSGSWDAERLAGAMKDLPPHEAYALALAEMKKTFQDEIAVLKEE
                     IRLLKAKGHKSI"
     gene            4585..5175
                     /locus_tag="PLQ77_06285"
     CDS             4585..5175
                     /locus_tag="PLQ77_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10319.1"
                     /translation="MPGKTEWIDLKREKRELDEFLSAIAGDEAVLPEENVFTSGDVLA
                     QDKPDAVSGETKAEKETQTAEDGGSLPDADGASPPEELTGAMNPPLSADAAPREPEEP
                     AAPAAEEEISVPLGSIETMAKFDDAMEPVPPPEKRKAFTPASAPRKEDGGILYWIKMA
                     AAFFITMTLAVLAGYFWVYPERGAQVIDRIQSLLMG"
     gene            5520..6818
                     /locus_tag="PLQ77_06290"
     CDS             5520..6818
                     /locus_tag="PLQ77_06290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPL10320.1"
                     /translation="MSFEKFKFNEKILAGVAAAGYRKPTPIQAESIPPIMEGRDVMGL
                     AQTGTGKTAAFVLPILHRLMQKKRGPIRALIVAPTRELSEQTHASIMELGRKTGICST
                     AIYGGVSIYNQVNRLRSRVDIVSACPGRLLDHIGRGTLDLSHVEVLVLDEADHMFDMG
                     FLPDVRKIVGHLPAKRQTLLFSATMPGEIRSLADDLLCDPVHIQIGNSAPVMTVAHTF
                     YPVQTEQKTALLKDILEKIDTRSVLVFTRTKSRAKSLAEQLNRAGHNKVTSLHGNLTQ
                     QKRKQSLDGFRDGRFDVMIATDIAARGIDVTGISHVINYDMPSTADAYTHRIGRTGRA
                     AMTGDAFTFITGQDGDLLRSLKKLLGEKLQYQNVDGLTVSPSVCEATSARAHQLTRPG
                     NHSGRKPFSARTPQARRPAQRSFKDAGRMNWASRARQAAV"
     gene            complement(6906..8597)
                     /locus_tag="PLQ77_06295"
     CDS             complement(6906..8597)
                     /locus_tag="PLQ77_06295"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HPL10321.1"
                     /translation="MPSILPDNIFNLAAVVREVALQDPERTAVIEPDGFDAQGRRRYK
                     RRTYTELSNDAEAVALGLREAGIAEKTRIVCMTPPGYEACALGLALQRVGATTLWIDP
                     SVGFCNVGERLRRIRPEGFAGVPISHLARILFGWGPRFPRHAIVIDGWWPGAHTLASL
                     KRTPPARPAPPAVKPDDPAAVLFTTGSTGPAKPALYLHRNFAHLYRIVNESWRFETAR
                     GIPVDMAVFPAFFFIALSAGGTMVVPPINFIFETPAKADPRALVDVINDCGVRSCFGS
                     PVLLENMARYAAAHDIKTPTFTRVIGGGAPIFGPVMQALLDMMGPRAEVFSNYGATEA
                     LPTSEMSAQEVLAETWPLTLQGAGICVGRPFTGVELRIIEMSDGVIEHIEQARLLEAG
                     EIGEILVRSKHVSPAYLDDPENTRKNKIYGPDGEIWHRIGDAGYLDFKGRLWYCGRVS
                     QRVKAGEGPLFSLNCEPIFDAHPAVRRSGLVGIPKDGAEEPVICVELRKEHRGNEETI
                     QNELLTLAAGHEATKQIKHVLFPATLPTDPRHNSKIERPKLAKWAAAKIRFRTNG"
     gene            complement(8597..9454)
                     /locus_tag="PLQ77_06300"
     CDS             complement(8597..9454)
                     /locus_tag="PLQ77_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL10322.1"
                     /translation="METFDYQGAAVKYKRRGSGEPVVFLHNGGTSHAIWKDVIARLAD
                     DYATFALDLPGFGASAKPGGGYALENYVAVLEAFLAHHGLSPVTLVGNCMGSAISVAY
                     AMRHPEKVRALVLVNPLTAATYTAGRLGMTLRLRRRAPAFSRFLYGLLGRVKPNRWIA
                     VQSLRMQFGPVGRSQNLHCSNDLCACFTGRGQMTSLLGVLDDLVNYEFLDNFVPPDDF
                     PPRCTIWGLENEILSAKAGRRLNATLQPQRADWLKGVGHLLMLENPDEVARIIRELLA
                     KTPRRKVHS"
     gene            complement(9455..>9780)
                     /locus_tag="PLQ77_06305"
     CDS             complement(9455..>9780)
                     /locus_tag="PLQ77_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10323.1"
                     /translation="QKRPYRYLLRPKAGGIALALSVLGDKFNSLLDITIVYPDGIPTF
                     WEFLSGGVRRVVVRISSIEIPSHFLNMDYEGDREFQAVFQQWVQDLWKDKDAQIDALL
                     AEVKA"
BASE COUNT         2198 a         2741 c         2918 g         1923 t
ORIGIN      
        1 tgggtcacat caatattttt ccggaccggg acggcacggt gcgcagcgaa gcgctgttga
       61 tcaattttga agagaagctt ttcccgtcgt tcgctcttca actgacgctg aaatacctca
      121 attacgatgt gaagcaggtg cgtccgggca aaaccgtcac cttcggccgc aaaagcattc
      181 ccgtgtatga aaacaacaga atgctgatca gtttcaacga ggggattccc tattattcat
      241 acttcgacgt cgtcaacaag aagatcgctc cggaggtgtt caaggacaag atcgtgatta
      301 tcgcgctctc cgcggcgggg ctgggcacca tgcaggtcac gccggtcgcc gtcaatgtgc
      361 cgcccgggac cgtcatcgcc aacgtgatcg acaacatcat caacggcgat cacgttatcc
      421 gtccggattg ggcgctcacg ctggaactca tcatgatcct gatcttcggc ctttacgttg
      481 cgctggtcat tccgcatatc aaggccggca tcagcgcggt ggtctccctg gctctgcttc
      541 tggtctggat gatcgcgacg atttatcttt ttgccgccta cgggtactgg atcaaggcgc
      601 tgtatccggc gcttctcctg ctcgtcggct acatcgtgat cgtctccaag cggtatctgc
      661 tgacggaaaa agcgaaggat cgcatggaag cggacggcgt cgaaaccaac aaaatgctcg
      721 gactgtcctt ccagggccag ggtctgctgg acatggcgtt tgacaaattc cgcaagtgtc
      781 ccgtggagga cgaatccgtg aaggagctgc tctacaatct cggcctggac ttcgaacgga
      841 agcggatgtt caacaaggcg gcggcggttt accagcatat cgcccaggac ggagatttca
      901 aggacatcaa cgaccgcatc aagaagctga cggccgccgg ggaaaccatg attttcgggc
      961 tttccggggc caaaaaagaa gccacggtca ttatggagaa caccgaaatc aagccgacgc
     1021 tcgggcgcta cgaaattgtc aaggagctgg ggcgcggcgc catgggaacg gttttcctgg
     1081 gcaaagaccc gcgcatcaac cgggaagtgg ccatcaagac gctgcgctac gaggagattg
     1141 actccgaaca gatcgacgag gtgaagaaaa gatttttccg ggaggcggaa gcggccggca
     1201 agctgtccca tccgaatatc gtgaccattt tcgatgtcgg cgaggattac gacattgcgt
     1261 atatggccat ggaactgctg gacggatcgg acctggccaa gtacactcga aaggaaaatc
     1321 tcctgccgcc cccggaaatc gtgcggatcg tttcctcggt cgcttcagcc ctggactacg
     1381 cccacgccaa cggcatcgtg catcgcgaca tcaagccggc caatatcatg attctgacga
     1441 acggcgaggt caaggttgcg gatttcggca tcgcccgcgt gatggccacg tcgaaaacgc
     1501 agaccggagt ggtcctcgga acgcccagct acatgtcgcc ggagcagatt gccggcaaga
     1561 aggtggacgg gcgatccgat ctgttttcgc ttggcgtggt tctctatgag ctgatttcag
     1621 gagaaaaacc cttcaacggc gacagcattg ccacgctgat gtacaacatc acgaccacgg
     1681 atccccgggc catcaaagag ctgtctcccg accttccgga cgccatggcg gggattgtgg
     1741 aaaagctgct ggccagggat gtggaggccc gataccagac cgggaaagcc ctggcggatg
     1801 atttgcttgc ctgtttaaaa taaggaggct cccttgaatc ttacggcctg cggaaaaacc
     1861 gatcaggggt tggtcagaac caacaacgaa gacaattttt attcggatga gaagatgggg
     1921 cttctggtcg tggccgacgg catgggcggc cacgcatccg gagagaaggc cagcagtctg
     1981 gcggtcagcg tcattcggga ttattttcag gggcagcaaa tcatgatcgg cgatcgtgat
     2041 ccctcgtttt ccgaggccac caacaagctc gcctgcgcca tccgtctggc caatcaggcc
     2101 gtttacgagg cttcacaaag ttcaccgcaa ctcaagggga tgggaaccac cattgtggcg
     2161 gtgctgctga caggccggaa actgagcatc gcccacatcg gcgacagccg ggcgtacctg
     2221 gtccgcgccg gcaccatgga acagctgacg gacgatcatt ccatggtgaa cgagcaggtc
     2281 aagcgcaatc tgatctcccg ggaagaagcc gacctctcgg agatgaagaa tattctcacg
     2341 aaagccctgg ggattcagcc ggagctggac gccgatctcg atgaactgac cctgctggaa
     2401 ggcgatattt tgctcttgtg tacggacgga cttagtaata tggtgacgga cgacgacgcg
     2461 ctggacatga tctcttccgc cgccgacgct tccgccgcct gtgaatccct gattgaagcc
     2521 gccaataaaa acggcgggaa agataatatt accgttgtga tcggttatgc ctggaaggaa
     2581 aagtggtatt cggctttgat gaaattcatg gaatttttcc ggaggtgatc tatggcaaaa
     2641 atattactga agtacaaaga ggccgccgtt aaggaaatcc cgctggacaa ggaatccacc
     2701 acgatcggcc gcaagccgga taacgacatt gtcatcgaca atcaggccgt atccggtcac
     2761 cacgcctcca tcacggtgga aggcgacaag ctcatgctgg aggatctggg cagcctgaac
     2821 ggaaccttcg taaacggcca gaaaatctcc aaaacggagc tgttcaacgg cgacgtggtg
     2881 ctcatcggcg tgcataccct gaatgtcacc tccgaaaaaa acaggtcgca ggaaaccaag
     2941 ggtttcgcgg tgcgcggccg ctccatggat gaaacgatgg tgatcgcgcc ggatgaccag
     3001 aagaaaatcg tcgcggcagc ggacaagaac cttccggaac cgctgggcgg ctttatggtc
     3061 gtggaaggat cgacggagaa gaaagaatac gaactgaagg aaagagtgtc ctcgattgga
     3121 aaggaagacg gatcggccgt caaattgaag gggctgttcg ctcccaagct ggccgccctg
     3181 gtcaaccgcc ggaaagaagg ctattttatt acgccggccg gcggcaagga gctcaaggtc
     3241 aacgggaaaa ccattgaccg ccgctgcgat ctcaaagacg gcgatctggt ggaagtcgcg
     3301 aatttgaaac tgcagtttta tctcaaagag tagggggtca gcgctcaccc gtcccaaaga
     3361 tcactccgcc cgggcggccc ccttccgctt tctttgcggt cttgcgaatg aacgcctgat
     3421 ctggtaggaa gcggatcggt aatgccttga cgtttttgaa gcagagggtc gccggttttt
     3481 tcggaaatac aggagtgatc acacccatgg ccagtcatga agagtcgtct tttcttgagg
     3541 cggtagaaag ccgtttggac agcattttcg gggcggactc caaaccggcc cggaaaacgg
     3601 attccggcat cccggaagaa atcggtgacg gcgccgccga cggccttcgg gaggattccc
     3661 tttctcctgg aaccggaaag gcctcctccg ttttgggccc gaaagaagac agatccgcct
     3721 ttctttcgga aatcgacaag cgctttgagg cgatcttcgg cgacgagaca ccgccggccc
     3781 gggaggccca acgcgcgtcg gagccggatg atttgaaaga actgacggcc cgcgcggaag
     3841 cggaggaaag cgcaaaagaa gagcgtcttt cggagggtct cacgctgttt ccgtcattgt
     3901 ccgcggactt tcctttaaag aatctgcaaa ccattgttct ctccctggaa gaccgaatga
     3961 acgaatccgg cctggagcgg ctggacgatg aagtttgcag gctggaccgg ctcttggaaa
     4021 gcgatcccgt ccttcaggga ttattgcgca ttttgcgttt tgcgggaaga tacattcggg
     4081 tcaaggacga ccgttttggc ggtgatgccg cggcgctgct cttttcggtt tacagacgca
     4141 tggaacaggc gacgggcggg gaggaaatgg cgcaggaagc gaaacgccgc ttgctgcagg
     4201 aaagcattga acagtatcaa gcgtgggtgg aaaacgccga cctggagaaa cgaatcgaac
     4261 ccgaagcccc gaggggcatg acggacggcg aaagggcccg ggatgcggag cgcaggaatg
     4321 ttccaatgga tgaaatgccc gttgcccgca ttcgggtggt ggaggacaag tcgccgccgt
     4381 cagggagctg ggacgctgag cggctggccg gggcgatgaa agatctgccg ccccatgaag
     4441 cgtatgccct ggcgcttgcg gagatgaaaa aaacattcca ggacgagatc gccgtcctca
     4501 aagaggaaat tcgccttttg aaggccaaag gccataaaag catttagaca aaacggcgaa
     4561 ttcagggatg gaaggggagc cgcaatgccc ggtaaaacgg agtggatcga tttaaagcgg
     4621 gagaaacggg agctggacga atttttgtcc gccattgccg gcgatgaagc ggttcttccc
     4681 gaggaaaatg tgttcacctc cggcgatgtt ctcgcgcaag acaaaccgga cgcagtttcc
     4741 ggggagacca aagccgaaaa agagactcag acggcggagg atggcggctc gcttcccgac
     4801 gcggacggag cttcgccgcc tgaagaattg actggcgcaa tgaatccgcc gctttccgcg
     4861 gatgccgccc cccgcgaacc ggaagaaccg gccgcacccg ccgcagagga ggaaatatcg
     4921 gtgccgcttg gcagcatcga aacgatggcc aaatttgatg atgcgatgga gcccgtgccg
     4981 ccgccggaaa agcgcaaagc cttcacgcct gcatctgcgc cccgaaagga agacggcgga
     5041 attctgtact ggatcaaaat ggccgccgcc tttttcatca ccatgaccct ggccgttctg
     5101 gccggatatt tctgggtgta tccggagcgg ggcgctcagg tcatcgacag gattcaatcc
     5161 ttgctgatgg gttgacaaac cggcccaggc ccctccccgg aaaaaaaggc ccggggcaat
     5221 cccggcgctt ttcatttgcg cccgcattcc aaagataatg cttgctattt gccggctttg
     5281 cctgtaggaa cccctcagga atagcgaaaa aattgacctt cccacctttc ttcatcggaa
     5341 atttgctaag gataatcccg ctaatcgaaa tagaaaccct gatgccatgc cgtctttgag
     5401 acctgtcttt cagacgcagg acgtgcggat gaaaaggcct gtttttacca gccttgtcac
     5461 ggttcgcatc gggtttaact ttaaatagat gctggtaact tacaaaaaaa ggataaaaaa
     5521 tgagttttga gaaatttaag ttcaatgaaa aaatactggc cggcgttgcc gccgccggtt
     5581 accgcaagcc tacgcccatt caggcggagt ccatccctcc catcatggaa ggcagggatg
     5641 tgatggggtt ggctcaaaca ggcacaggga aaaccgccgc ttttgtgctg ccgattctgc
     5701 accgcctgat gcagaaaaag cgcggcccga ttcgcgccct gatcgttgcg ccgacgcggg
     5761 aattgagcga acaaacccat gcatccatca tggaactggg aaggaaaacg gggatttgca
     5821 gcacggcgat ttacggcggc gtcagcatct ataatcaggt caatcgactg cgttcccgtg
     5881 tcgatatcgt ttcggcctgt cccggccgcc tgctggacca tatcggccgc ggcacgctgg
     5941 atttgtcgca tgtggaagtt ctggtgctgg atgaggcaga ccacatgttt gatatgggtt
     6001 ttctgccgga tgttcgcaag attgtcgggc atttgccggc aaagcgccag acgctgcttt
     6061 tctccgccac catgcccggc gaaatccgca gcctggccga tgatctgctt tgtgatcccg
     6121 ttcacattca gattggaaat tccgctccgg tgatgacggt tgcccacacg ttttatccgg
     6181 tgcaaaccga gcagaaaaca gccttgctca aggacattct cgagaaaatc gacacccggt
     6241 cggtcctggt gttcacccgg acaaaatcgc gcgccaaaag cctggccgag cagctcaaca
     6301 gagccggaca caataaagtg acctccctgc acggcaatct tacgcagcag aaacgcaagc
     6361 agtccctgga tggattccgg gatggccgct ttgatgtcat gatcgccacg gacattgccg
     6421 cgcgcggcat cgacgtgacg ggcatctccc atgtgatcaa ctatgatatg ccgtccaccg
     6481 cggatgccta tacgcaccgc atcggcagaa ccggacgcgc cgcaatgacc ggggatgcgt
     6541 ttacctttat caccgggcag gatggcgacc tgctcaggtc cctcaagaaa ctgctggggg
     6601 aaaaactgca atatcagaat gttgatggat taaccgtcag cccgtcggtt tgtgaagcaa
     6661 cgagcgctcg cgctcatcag ctgacgagac ccggcaacca cagcggcagg aaaccgtttt
     6721 cggcccggac gccgcaggcc cgccggcccg ctcaacgttc gtttaaagac gccggccgga
     6781 tgaactgggc atcccgcgcc cggcaggcgg cagtctaaaa aatatcattt atccattgat
     6841 gtggaagaag ttcacgcggt cggcgaagcg caggttttcg cgggaatcac ggacttcttt
     6901 ccgcgtcatc cattcgtccg aaagcggatt ttggcggcgg cccattttgc cagcttgggt
     6961 cgctcaatct tggaattgtg ccgggggtcc gtcggcagcg ttgccggaaa gagaacgtgc
     7021 ttgatttgct tcgtcgcctc gtgtccggcc gccagagtga gcagctcgtt ttggatcgtc
     7081 tcctcgtttc cacggtgctc tttgcggagt tccacgcaga tgaccggctc ttccgcgccg
     7141 tccttcggaa ttccaaccag cccggaccgg cgcacggcag gatgagcgtc gaagatcggt
     7201 tcgcagttga gggagaacag cggtccttcc ccggctttca cacgctggct gacgcggccg
     7261 caataccaaa gccggccttt gaaatccagg tagcccgcat caccgatgcg gtgccatatt
     7321 tcgccatccg gcccatagat cttgttcttt cgagtgtttt cgggatcgtc cagataggcg
     7381 gggcttacat gcttgctcct gaccaggatc tcgccgattt cccccgcttc gagcagccgc
     7441 gcctgctcga tgtgttcgat gacgccgtca ctcatttcga taatgcggag ctcgacgccc
     7501 gtaaagggcc ggccgacgca gattcccgcc ccctgcaggg tcaacggcca tgtttcggcg
     7561 agcacttcct gtgcgctcat ttcactggtg ggcaaagcct ccgtcgctcc gtaatttgaa
     7621 aagacctcgg cccggggacc catcatatcc aacagcgcct gcatgacagg gccgaagatt
     7681 ggcgctcccc cgccgatgac acgggtgaag gttggcgttt tgatatcgtg ggccgcggca
     7741 taccgggcca tgttttccag caggaccggc gagccgaaac acgaacgcac gccgcaatcg
     7801 ttgatgacgt cgacgagggc cctggggtcg gctttggcag gcgtttcaaa aatgaagttg
     7861 atgggtggaa caaccatcgt gccgccggcg ctgagggcga tgaagaagaa agcggggaag
     7921 acggccatgt cgacgggaat gccgcgcgcc gtttcaaatc gccaactttc gttcacgatg
     7981 cggtacaggt gcgcgaaatt tcgatgcagg tagagagccg gctttgcggg tccggtgctg
     8041 ccggtggtga aaagcacggc cgcaggatcg tcgggtttga cggcgggcgg agcgggccgg
     8101 gccggcggag ttcgcttgag ggatgccagg gtatgcgcgc cgggccacca gccgtcgatg
     8161 acgatcgcgt ggcggggaaa acgcggtccc cagccgaaca atatgcgcgc caggtggctg
     8221 atgggcacac cggcgaagcc ctcgggccga atccggcgca ggcgttcgcc gacattgcaa
     8281 aaaccgacgg acggatcaat ccacagtgtc gtcgctccca cccgctgcag cgccaggccc
     8341 agggcgcaag cttcgtagcc gggcggggtc atgcagacga tgcgcgtttt ttcggcaatg
     8401 ccggcttcgc gcagcccgag cgcaacggcc tcggcatcat tggaaagctc ggtgtaggtg
     8461 cggcgcttgt agcgccgcct gccttgcgca tcgaagccgt ccggttcgat gacggcagtg
     8521 cgctcaggat cctgcaaagc cacctcgcgc acgacggccg ccaggttgaa gatgttgtcc
     8581 gggagaatcg agggcatcac gagtgcacct tccgccgcgg agtctttgcc agaagctcac
     8641 gaatgatccg ggccacctca tcgggatttt caagcatgag cagatgtccg acacccttaa
     8701 gccaatccgc tcgctggggt tgcagggtgg cattgagccg ccgtccggct ttggccgaca
     8761 ggatctcgtt ttcaaggccc cagatcgtgc agcggggcgg aaaatcgtcc ggcggaacga
     8821 agttgtccag gaattcgtaa tttaccagat cgtcgagtac gcccaaaagc gacgtcattt
     8881 gcccgcggcc ggtgaaacag gcgcacaggt cgttgctgca atgcaggttt tgcgaccggc
     8941 cgaccggccc gaattgcatg cgcaaagatt ggaccgcgat ccagcggttg ggcttcaccc
     9001 ggcccagcag gccgtacaga aagcgtgaaa aggccggtgc tcgtcgtcgc aaacgcagtg
     9061 tcatgccgag ccttccggcc gtataggtcg cggcggtgag cgggttgacg agcaccaggg
     9121 cgcgcacctt ctcggggtgc cgcatggcgt aggcaaccga aatggcgctg cccatgcaat
     9181 tgccgaccag ggtcaccggc gaaagaccgt gatgcgccag aaatgcctca agaacggcga
     9241 cgtagttctc cagcgcatag ccgccgcccg gcttggccga tgcgccgaag cccggaaggt
     9301 ccagggcaaa ggtcgcataa tcgtcggcca gcctggcgat cacgtccttc cagattgcgt
     9361 gcgatgttcc gccgttgtgc agaaagacca ccggttcgcc cgatccgcgc cgcttgtatt
     9421 tgacggcggc cccctggtaa tcaaaggtct ccatttatgc tttgacctcc gcaagcaatg
     9481 cgtcgatttg cgcatccttg tccttccaca aatcctgcac ccactgctga aagacggcct
     9541 ggaattccct gtcgccttcg taatccatgt tcagaaaatg cgaagggatt tcaatggagg
     9601 atattcgaac gacaaccctc ctcacgccgc cggacaaaaa ttcccagaaa gtgggaatgc
     9661 cgtccggata aacaatcgta atatccagca gagaattgaa tttatccccc agcacggaga
     9721 gcgccagcgc gatgccgccc gctttgggcc tcagcaggta gcggtaaggt cttttctgat
//
LOCUS       DAOPAF010000059         9700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6690_length_9700_cov_63.965474, whole genome shotgun sequence.
ACCESSION   DAOPAF010000059 DAOPAF010000000
VERSION     DAOPAF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6690_length_9700_cov_63.965474"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLQ77_06310"
     CDS             <1..925
                     /locus_tag="PLQ77_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL10324.1"
                     /translation="GALPFFHVFGMSCAMNFSIIQGWGDILIPKPQPEPLLDAIRKFK
                     PNFGALVPTMFIGMLNHPKMKTTDMTCFKGFFSGSAPLPVEVIHDFEKTAGSVICEGF
                     GMTETTPVTHTNPFEGIRKIGSIGLPISDTECRIVDLDKGETDMPVGEPGELIIRGPQ
                     VTKGYLNKPEETAGLLRDGWCYTGDIATMDQDGYFYIVDRKKDMIISGGFNVYPRDID
                     EAYYEHPKVMEACAIGIPDAKRGENVKLFIVLKEGETATAEEMIEYGKSKLATYKLPT
                     EVEFRKELPKSTVGKVLRKELRAEELAKRKK"
     gene            complement(997..1392)
                     /locus_tag="PLQ77_06315"
     CDS             complement(997..1392)
                     /locus_tag="PLQ77_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPL10325.1"
                     /translation="MKILVAYVGGLDVDQAVLEVAKKHAKAFNASLYVASSMERVSER
                     ERRDLDKVEKQLAYVKEMMDAERIECETHILVRGLTPGEDIVEFAADKKVDEIIIGIE
                     KKSKVGKLLFGSNAQYIILQSACPVVSVK"
     gene            complement(1605..4550)
                     /locus_tag="PLQ77_06320"
     CDS             complement(1605..4550)
                     /locus_tag="PLQ77_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10326.1"
                     /translation="MNDPFKTGLKSTQDHPAFNQFTANVSREVLNRIILILSILTGLG
                     LIAAIFLFQKKWGSAAMLLALMAILITAKLLMRRGQLRLAAILTIAGVWLVFGVNMLL
                     GGGVYNVNAVFFLVMTVIAGLLFGEKATFWAAAASIAALFVLALLGFFGLLPSPYFVE
                     SPLGNLAQLIFALVTTAGTLNLALGERDNALGMAHERLADQLKARRALQESERKHREL
                     VDFLPVAVFECDFEGNVLSGNLAIREMFGYSLADYEKGLNGFDMIHPNDRERAAQNLQ
                     ALVRGDKKDPFSEYTALHKDGTAFPCAFFTSVILEEKKPVGFRGAIIDLSSQKQAREA
                     LRQAEEKYRTIIEQMVEGYFELDLAGSYTFANDAECKIAGCSREELIGMNNRQFQDKE
                     NAKKAYQVFNRIYKTGKTVKSLEVEIIKKDGSKGFNEISVSLRRDAEGRPIGFRGFTR
                     DVTERRQAEENLRFSEERFRGIASNLPGGVFQFFARHNGDMGLNYISENAQDLLGLPH
                     DPEQFFPKLAACIAPEDRERFLESINEAIRSVSRWSFEGRYIKPTGEEMYLRGISAPI
                     EIKDGLIFNGVILDITDRRQAEAEKRRLEERLRRAEKMEALGQLAGGVAHDLNNVLGV
                     LSGYSELLLAEIPEGQKVRKHVEKILQSTEKGAAIIQDLLTLARRGVMSAEVLQINET
                     ISGFTKTPAFENIRDYHPGVTFRTECDPNLLNIKGSPVHLEKTLMNLVSNAAEAISGE
                     GEVTIRTENRYLDKAIVGYDEVKEGDYAVLIVSDTGAGIADEHREKIFEPFYTKKAMG
                     RSGTGLGLSIVWGTVKDHSGYIDVKTKVGAGTSFTLYFPATREETNLPTPRTPLERYM
                     GRGETVLVVDDIAEQRDVASGLLKKLGYYVHAVSSGEAAVEYLKENRADMVILDMIMA
                     PGMDGMETYRKILAIHSQQKAILVSGFSETDRVRTAQRLGAGAYVKKPYVMETIGLAI
                     RKELDRAAV"
     gene            complement(4676..5968)
                     /locus_tag="PLQ77_06325"
     CDS             complement(4676..5968)
                     /locus_tag="PLQ77_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL10327.1"
                     /translation="MIHSSPTTRPAFSGWTTLAGVMLAYGAICGAVTYGFGVFLPSMS
                     ESFGWSRSALSGPYTLFLMIGGLLGPLAGVTVARFGARKNIILGNAAAALGLLGMSQV
                     GEIWQVYLFFGVMGGLGLAFAEFLAATTVINHWFVRKRSLALGFLFASGGIGGFLMPP
                     FISALISGLGWRFAWAALACVHLIMGVVGAGWLIRNKPEEEGQFPDGHASQEQFDAWR
                     EKETGPVYHTREDWGVRDALRTPALWILLCLFSVLLFATTMLTAHQVAYLQDLHYSPM
                     VSATALGLMLGTSILGRLLSGVLGMRFKGMHLAAVFMACMGFGFLSLMNARDLFFVYL
                     YSILTGLGFGGMIVLLPHTMGAYFGRANFSRIVGWTTPIVTLASAASPLLAGFLHDST
                     GSYTLPFAVATALIFACCLLALAARPPKPRNKASNPRA"
     gene            6158..7471
                     /locus_tag="PLQ77_06330"
     CDS             6158..7471
                     /locus_tag="PLQ77_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPL10328.1"
                     /translation="MTGSDVLAVVGHSGVVIPPEISLEDLTEEFTALLKNVDWYTQWL
                     YDFRDILGNRQVVFPYCSILLDANRDPADLDECVPVRDVFGRPIYRSAYEPSPSMRAA
                     WSDKYLKPFHRGIEENISAGAGLLFDGHSTVTARGVAANQIDLMNFQQTDREEKPLYY
                     CPDVIVETYAEELRKRLPDALVTVNASEYLSVHGHICAAHSVNSLKRIGARAPAFIQE
                     TNENLFKNSDGTPNVGRINRLRRAFAESLAQTLQSLQESQKVTMIDLHLGKQIFDYDC
                     GVQALQTVMTYYGVEVDRDELMQTLGTTEESGTPPKAMIAAAQHYGFEVKSGTQWSLN
                     QVKQFVDAGTPVIVLLQAWAERYMTLDDWRSDWDNGHYAIVIGLNKDVLLFEDPATIR
                     RTWLREREFLARWHDMDVKTGEKYEHFGMVLLGKQPAKLSLEHMD"
     gene            complement(7486..9309)
                     /locus_tag="PLQ77_06335"
     CDS             complement(7486..9309)
                     /locus_tag="PLQ77_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPL10329.1"
                     /translation="MNSHRREPFWSGVVRSMGIVFGDIGTSPIYTLTVVFALTPRTEA
                     SVLGILSLVVWTLLILVTGEYAWLAMNLSYKGQGGEIMLREILRRALKPGRKLAFAGF
                     LAFIGVSLLLGDGVITPAITLLSAVEGIALIPGLENVRWEILILIAVFIAVGLFSIQS
                     RGVDKVAGIFGPVMALWFLCLAVTGLVSIAAMPGILKAVNPYYAFAFLSENGLAGFFV
                     LSEVILCATGGEALYADMGHLGKKPILHAWYFVFVAILLNYMGQGVFAFLHPDVKYLL
                     FGMVQHQAPFLYIPFLLLTFLATIIASQSIISGTFSIVYQGITTRILPLMKVDYTSSR
                     IKSQIHIPFVSWSLMVAVIFMILLFQKSENLAAAYGMAVTGSMTITGLMMILIFSQIK
                     KMRWKLPAAVFITGIAFAYFLSTLSKLPHGAYWSLILAAVPLTTILIWTKGQKRLFKA
                     LRPLDWETFFISYQQIYAKGRNILGAALFFCRGTQMISPYIVHSIFRSNIIYERNILI
                     SINRTDEPYGVAVHHKPDLGPGLEALEIEAGYREVLDIEALIKEQGIQEKVIFYGVED
                     IVTRNPIWGVFSLIKRISPNFVQFSKLPPSRLQGIVTRVEM"
     gene            9517..>9700
                     /locus_tag="PLQ77_06340"
     CDS             9517..>9700
                     /locus_tag="PLQ77_06340"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPL10330.1"
                     /translation="MTAPLCIAKSGEKEIVLLPAMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGAPVFLADVKG"
BASE COUNT         2187 a         2733 c         2771 g         2009 t
ORIGIN      
        1 gggcgcgctg cccttcttcc atgttttcgg catgtcctgc gcgatgaatt tctccattat
       61 ccagggatgg ggcgacatcc tgattcccaa accgcagccg gagccccttc tggatgccat
      121 ccgcaagttc aagcccaact tcggcgcgct ggttccgacc atgtttatcg gcatgctgaa
      181 tcatccgaag atgaagacga ccgatatgac ctgcttcaaa ggcttcttct ccggcagcgc
      241 cccgctgccc gtggaagtga ttcatgattt tgaaaaaacg gcgggatcgg tcatctgtga
      301 aggcttcggc atgacggaaa ccacgccggt aacgcacacc aacccgtttg aagggattcg
      361 gaaaatcggt tcgatcggcc tgcccatatc ggatacggag tgccgcatcg tcgatctgga
      421 caagggcgag acggatatgc ccgtaggcga gccgggcgaa ctcattatcc gcggtccgca
      481 ggtgaccaag ggctatctga acaaaccgga ggaaaccgcg ggactgctca gagacggctg
      541 gtgctacacg ggcgatatcg ccacgatgga tcaggacggc tacttctaca tcgtggaccg
      601 caagaaggat atgattatca gcggcggatt caacgtctat ccccgtgata tcgatgaagc
      661 gtactacgag catccgaagg ttatggaagc ctgcgccatc ggcattccgg atgcaaaacg
      721 gggagaaaac gtcaagctgt tcatcgtact gaaagaaggg gaaacggcga cggccgaaga
      781 gatgattgaa tacggcaagt cgaaactggc cacctacaag ctgcccacgg aagtcgaatt
      841 ccgcaaggaa ttgccgaaat cgaccgtcgg caaggttctg cgcaaggaac tgagggcgga
      901 agaactggcc aagcgcaaga agtagccgcc cgctcgtcgc cagagacaaa aaaggcttgc
      961 cccgcggggc aagccttttt tttaagacgg ctgttgttat tttacggaaa ccaccggaca
     1021 ggcggactgc agaataatgt attgggcatt ggatccgaac agcagtttgc cgactttgga
     1081 ttttttctcg atgccgatga tgatttcgtc cactttcttg tcggccgcga attccacgat
     1141 gtcttctccg ggggtgagtc cgcgcaccag aatatgcgtt tcgcactcaa tcctttcggc
     1201 atccatcatt tccttgacgt aagccagttg tttttccact ttgtccaggt ccctgcgctc
     1261 cctctccgat acccgttcca tggagctcgc gacatacagg gaagcgttaa aagccttggc
     1321 gtgttttttc gccacttcca gcactgcctg atcgacgtcc aaccctccga catatgcgac
     1381 taaaattttc attcttcccc ccccctcgtc cttcctgttg agtctggatg tttgtgcaca
     1441 tattctctgg tttttgtttg gccggagtat aagaaaaccc atctttaatg tcaatcggtt
     1501 tcttcttttg tacggccggt ttagaaatgt cctgtttcag ccgaaggcat gcttccccgt
     1561 catccgggaa ggcctgaacg gatcgtcata gaccgaacag ccgttcaaac cgctgcgcga
     1621 tccaattcct tccgaatggc caggccgatc gtctccatca catagggctt cttgacatat
     1681 gcccccgctc caagccgttg agccgtccgg acgcgatccg tttccgaaaa accgctcacc
     1741 agaatcgcct tctgctgtga atggatcgca aggattttcc tgtacgtctc cattccgtcc
     1801 atccccggcg ccatgatcat atccagaatc accatgtccg cccggttctc tttgaggtac
     1861 tccaccgccg cttccccgct ggaaacagcg tgaacataat atcccagttt tttcagcagc
     1921 ccggaagcca cgtctctctg ctcggcaatg tcgtctacga ccagaaccgt ctccccccgg
     1981 cccatgtacc gctccagagg cgtcctgggc gtcggcaaat tcgtttcttc acgggtggcc
     2041 gggaagtaca gcgtaaagga agtgcccgct ccgaccttcg tctttacatc aatgtacccg
     2101 ctgtggtcct tgaccgtccc ccagacaatc gacagcccca gacccgttcc gcttctgccc
     2161 atggccttct tcgtgtagaa cggctcgaaa atcttctccc ggtgctcgtc ggcaatgccc
     2221 gcccccgtgt cggatacgat cagaacggca tagtccccct ccttgacctc gtcgtagccc
     2281 acaatggctt tgtccagata gcggttctcg gtccgaatgg tcacttcccc ctcgccgctg
     2341 atggcttccg ccgcgttgga caccaggttc atcagtgtct tttccagatg gacgggggag
     2401 cctttgatgt tgaggaggtt cggatcgcat tccgtccgga aggtgacgcc cggatgatag
     2461 tcccgaatgt tctcgaacgc cggggttttg gtaaaaccgg aaatggtttc gttgatttgg
     2521 aggacttccg ccgacatgac gccccgccgg gccagggtaa gcaggtcctg tatgatggct
     2581 gcgcctttct cggtggactg gaggatcttt tcgacgtgtt ttcggacttt ctgtccttcg
     2641 ggaatttcgg cgagcaggag ttcggaatat ccggaaagaa cgcccaggac gttgttcagg
     2701 tcatgggcga cgccgccggc cagctgtccc agggcttcca ttttctcggc ccggcgcaaa
     2761 cgctcctcca gccttcgctt ctccgcctcc gcctgcctgc ggtcggtgat gtccaggatc
     2821 acgccattga agatcaatcc atccttaatc tcgatgggag cggaaatccc ccgcaggtac
     2881 atctcttcgc cggtcggttt gatgtagcgc ccttcgaaac tccaccggct gacggagcgg
     2941 atcgcttcat tgatggactc gagaaatctt tcgcggtctt ccggggcgat gcaggcggca
     3001 agttttggga aaaattgctc tggatcgtgg ggcagcccca atagatcctg ggcgttttca
     3061 ctgatgtagt tgagtcccat gtctccattg tggcgggcga aaaattgaaa gacgccgccg
     3121 ggcaggttgc tggcaattcc cctgaatctc tcttcgctga accgcaggtt ttcttccgcc
     3181 tgcctgcgtt cggtgacgtc gcgcgtgaaa ccgcgaaatc cgatgggtct gccttcggcg
     3241 tctcttctta gagatacgga tatttcatta aaaccttttg acccgtcttt cttgatgatt
     3301 tcaacttcca gggacttgac cgttttgccg gttttgtaaa tccggttgaa gacctgatag
     3361 gcttttttgg cgttttcttt gtcctgaaat tgccggttgt tcatgccgat caattcctct
     3421 ctggaacatc cggcgatttt gcattccgcg tcattggcaa aggtataact cccggcaagg
     3481 tcaagttcaa aataaccttc caccatctgc tcaatgatcg tccggtattt ttcttcagcc
     3541 tgacgaagag cttcccgggc ctgtttctgg ctggaaagat caatgatggc ccccctgaag
     3601 cccactggct ttttctcttc aaggatgacg ctcgtaaaaa acgcgcaggg aaaggcggtg
     3661 ccgtctttgt gaagcgccgt gtattcggag aacggatcct ttttgtctcc gcgcaccagc
     3721 gcctgaaggt tttgggccgc tctttcacgg tcgttcggat gaatcatatc aaaaccgttc
     3781 agcccttttt cgtaatccgc cagcgaatag ccgaacatct cccgaatggc gaggttgccg
     3841 gaaaggacgt tgccttcgaa gtcgcattca aagaccgcga cgggaagaaa gtccaccagt
     3901 tccctgtgtt ttctttcgct ttcctgaaga gctcgccggg ccttcaactg gtccgcaagc
     3961 cgttcatggg ccatgcccag cgcgttgtcc cgctcgccca gcgccaggtt cagcgtgccg
     4021 gccgtcgtga ccagcgcgaa aatcaactgc gccagattgc cgagggggga ttccacaaag
     4081 tagggggaag gcaggaggcc gaaaaagccc aaaagggcca ggacgaaaag cgcggcaatg
     4141 cttgcggcgg cggcccaaaa cgtcgctttc tctccaaaga gcagtccggc gatcaccgtc
     4201 attaccagaa aaaaaacggc attgacgttg tacacgcctc cgcccaaaag catattgacg
     4261 ccgaaaacca gccacacgcc ggcaatcgta agaatggcgg ctaaacggag ctgcccgcgc
     4321 cgcatcaaaa gtttggctgt gatgaggatc gccatcagcg ccagaagcat ggcggcgctc
     4381 ccccatttct tctgaaagag gaagatggcg gcaatcaacc caaggcccgt caggatggat
     4441 agaatcagga ttatgcgatt cagaacttcg cggctcacgt ttgccgtgaa ttggttgaag
     4501 gcggggtgat cttgcgttga tttgagccct gttttgaatg ggtcattcat cgaaggttcc
     4561 ttcatcggcc ggagatgctg gatggagacg ccaggatgat atttttaatg gtccgcgttt
     4621 ggggaaatct agcggatggc cggggaaatg tcaaaaagaa aatcggaggc cggtcttacg
     4681 cgcggggatt ggatgctttg ttccgaggct ttggcgggcg agccgcgagc gcaaggaggc
     4741 agcaggcaaa aatcagcgcc gtcgcaacgg cgaagggcag ggtatagctg ccggtcgagt
     4801 catggagaaa tccggccagc agggggcttg cggccgaagc cagcgtgacg atcggcgtgg
     4861 tccagccgac gatgcgggaa aaattcgcgc gcccgaagta ggcgcccatc gtgtggggca
     4921 gaagaacgat catgccgccg aagccgaggc cggtcagaat ggaataaagg taaacaaaaa
     4981 agagatcccg ggcgttcatt aaagacagaa aaccgaatcc catacaggcc ataaagacgg
     5041 ctgccaggtg catgccttta aatcgcatac ccaaaactcc gctcaaaagc cgtcccagga
     5101 tgctcgttcc cagcatcagt cccagggccg tggcggaaac catcggcgag tagtgcaaat
     5161 cctgcaggta ggccacctgg tgcgcggtca gcatggtggt ggcaaaaagc aagacggaaa
     5221 agaggcaaag cagaatccac agcgccgggg tccgcagggc gtcgcgcacg ccccagtcct
     5281 cccgggtgtg ataaacgggg ccggtctcct tttcccgcca cgcgtcgaac tgttcctgtg
     5341 aggcgtgccc gtcaggaaac tggccttctt cttccggctt gttgcggatg agccagccgg
     5401 cccccacaac gcccatgatt aaatgaacgc aggccagcgc cgcccaggca aagcgccagc
     5461 ccaggccgga aatgagcgcg ctgatgaaag ggggcatgag gaaaccgccg atgccgccgg
     5521 aagcgaaaag aaaacccagc gccagcgacc ttttgcggac aaaccagtgg ttgatgacgg
     5581 tggtggccgc cagaaactcg gcaaaagcga gcccgaggcc ccccatcacg ccgaaaaaaa
     5641 gataaacctg ccagatttcg ccaacctggg acattcccag cagaccaagc gccgccgcgg
     5701 catttcccag aataatattt ttccgggcgc cgaaccgggc gacggtgacg cccgccagcg
     5761 gccccagcag gccgccgatc atgaggaaca gcgtataggg tccggacaga gcggaacggc
     5821 tccatccgaa tgattcgctc atcgacggca gaaagacgcc gaagccgtac gtgacggcgc
     5881 cgcagatggc cccgtaggcc agcataacgc ccgccagcgt tgtccagccc gaaaatgccg
     5941 gccgcgtggt tggtgatgaa tggatcatgc cgatccctgg ttacccgcct ttatgaagcg
     6001 cgactatagg aagaacgccc gcgcctgtca atcaataaac ataagtatat gatatcatag
     6061 agtatgtttt gcgtattcaa agagggataa aagcgctaca atacacggca aaatggagtc
     6121 tttcgcagaa gggactccgt gatcagaggt ttgcaaaatg acaggatcgg atgttttggc
     6181 agtggtcggc cacagcggcg tcgtgattcc gccggagatt tccctggagg atttgacgga
     6241 ggagtttacc gctctgctca aaaacgtgga ctggtacacg cagtggctgt atgactttcg
     6301 cgatattctc ggaaaccgcc aggtggtttt tccctactgc agcattctgc tggatgccaa
     6361 ccgggatccc gcggatctcg acgaatgcgt tccggtccgt gacgttttcg gccgtccgat
     6421 ttaccgcagc gcctacgaac cgtcgccctc catgcgggcg gcctggtcgg acaagtatct
     6481 gaaaccgttc catcgcggca tcgaggaaaa tatttccgcg ggcgcaggtc tgctctttga
     6541 cggccattct acggtgacgg cgcgcggcgt ggcggcaaac cagatcgatc tgatgaattt
     6601 tcagcagacg gaccgcgagg aaaagccgct gtactactgc ccggacgtca tcgtggaaac
     6661 ctacgccgaa gagctgcgca aacgcctgcc cgacgcgctg gtgaccgtga acgcttcgga
     6721 atacctgtcg gtgcacggcc atatctgcgc ggcgcactcg gtcaacagcc tgaagcggat
     6781 cggcgcgcgc gctcccgcgt tcattcagga aaccaacgag aacctcttta aaaacagcga
     6841 cgggacgccg aatgtcggac ggatcaaccg gctcaggcgg gcgttcgccg aatcgcttgc
     6901 gcagacgctc cagtcgcttc aggaatcgca gaaagtcacg atgatcgatc tgcacctggg
     6961 caagcagatc ttcgactatg actgcggcgt gcaggcgctt cagacggtga tgacgtatta
     7021 cggcgtggag gtggaccgcg acgagctgat gcagacgctc ggcacgacgg aggaaagcgg
     7081 cacgccgccc aaggccatga tcgcggccgc gcagcattac ggctttgaag tcaaatcggg
     7141 aacgcagtgg tcgctcaacc aggtgaagca gttcgtggat gcgggcacgc cggtcatcgt
     7201 tctcctgcag gcctgggcgg agcgctacat gaccctcgat gactggcgca gcgactggga
     7261 caacggccat tacgccattg tgatcggcct caacaaggac gtgctgctgt tcgaggatcc
     7321 ggccaccatt cgccgcacct ggctgcgcga gcgggagttt ctggcgcgct ggcacgatat
     7381 ggatgtgaag accggagaga aatacgagca tttcggcatg gtgcttctgg ggaaacagcc
     7441 tgcgaagctg tcgctggagc atatggactg acgacgccgc gcgggctaca tttccacccg
     7501 tgtcacgatc ccctgcaggc ggctgggcgg cagcttgctg aactgaacga agttgggcga
     7561 aatccgcttg atcagactga agacgcccca gatgggattg cgtgtgacaa tatcctccac
     7621 gccgtaaaag atgacctttt cctgaatgcc ctgctccttg atcaacgcct cgatatccag
     7681 aacttcccgg taaccggctt cgatttcaag agcttcgagg cccggcccca gatccggctt
     7741 gtgatgaacc gcaacgccgt agggttcatc ggtgcggttg atcgaaatca ggatgttgcg
     7801 ctcgtagatg atgttgctgc gaaaaatgct gtggacaatg taaggagaga tcatctgggt
     7861 tccccggcag aaaaacaggg ccgcgccaag aatatttctc cctttggcgt agatctgctg
     7921 atagctgata aaaaaagttt cccaatccag cggacgcagc gccttgaaaa ggcgtttctg
     7981 ccctttcgtc cagatgagaa ttgtcgtcag agggacggcg gccaggatga gcgaccagta
     8041 ggcgccatgg ggcagtttgc tcagcgtgga caggaaatac gcaaaggcga tgccggtgat
     8101 gaagacggcg gccggcagtt tccatctcat ctttttaatc tgtgagaaga tcaggatcat
     8161 catcagaccg gtaatggtca tcgatccggt tactgccatg ccgtaggccg cggcgaggtt
     8221 ctccgatttc tggaaaagaa ggatcatgaa aatgaccgcg accatcagcg accagctcac
     8281 gaacggaatg tggatctgcg atttgatccg gctcgacgtg tagtccacct tcatcagcgg
     8341 cagaatccgg gttgtgatgc cctggtaaac gatggaaaag gttccgctga tgatcgactg
     8401 ggaggcgatg atggtggcca ggaaggtcag caggagaaag gggatgtaga gaaacggcgc
     8461 ctggtgctgc accatgccga acagcaggta cttgacgtcc ggatggagaa aggcaaagac
     8521 gccctgaccc atgtaattga gcagaatcgc gacaaaaaca aaataccagg cgtgcaggat
     8581 gggctttttc cccagatggc ccatgtcggc gtacagggcc tcgccgcccg tggcgcacag
     8641 gatgacctcg gaaaggacaa aaaatcccgc cagtccgttt tccgaaagaa aggcaaaggc
     8701 gtagtaggga ttgacggcct tgagaatgcc cggcatggcg gcgatggaaa ccagccccgt
     8761 gaccgccagg cacaaaaacc acaaggccat gaccggtccg aaaatcccgg cgactttgtc
     8821 cactccccgg gattgaatgg aaaaaagtcc gaccgcgatg aagacggcaa tcaggatcag
     8881 aatttcccag cgcacgtttt ccaggccggg aatcagcgca atgccttcca cggcggacag
     8941 aagggtgatg gcgggcgtga tgacgccgtc gcccagcaga agcgaaaccc cgataaaggc
     9001 cagaaatccg gcaaacgcca gcttgcggcc gggtttgagc gctctgcgca ggatttcgcg
     9061 cagcatgatt tctccgccct gtcccttgta ggaaaggttc atggccagcc aggcgtattc
     9121 gcccgtgacg agaatcagca gcgtccagac caccagggaa agaatgccca gaacgctggc
     9181 ctcggttctc ggcgtcagcg cgaagacgac ggtcagggta taaatggggc ttgtgccgat
     9241 gtcgccgaac acaatgccca tggaacggac aacgccgctc cagaaaggtt cccgcctgtg
     9301 gctgttcaca tggcccccac gtaaatttga gcctgctcta ccaccgaaaa accgggcggt
     9361 caagcgccgt gaagggggcc ggaaaagtga ttttttgtgt ttttttgaag gatcgcgctt
     9421 ttcgaatgat gattgacaga cggccggatt ctttctatag tcgcgctcaa agaagttgaa
     9481 agatccatcg gcaaaaattc acatcaggag gccgtcatga ccgcaccgct ttgcatcgca
     9541 aagtccggag aaaaggaaat cgttctgctg cccgccatgg ccaaccgcca cgggctcatt
     9601 accggcgcaa ccggaacggg gaaaaccgtc acgctgcaaa aaatggccga atccttcgcg
     9661 tccatcggag cgccggtttt tctggccgac gtcaagggcg
//
LOCUS       DAOPAF010000060         9420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6912_length_9420_cov_56.633316, whole genome shotgun sequence.
ACCESSION   DAOPAF010000060 DAOPAF010000000
VERSION     DAOPAF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6912_length_9420_cov_56.633316"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLQ77_06345"
     CDS             complement(<1..608)
                     /locus_tag="PLQ77_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPL10331.1"
                     /translation="MTIKELDKAFEPAEAEKRLYDYWLENKFFHAQDTSEAPPFSIVI
                     PPPNVTGMLHMGHALNNTLQDIIIRFKRMQGYNTLWMPGMDHAGIATQNVVEQQLRKE
                     GLSRHDLGREKFIARVWEWKEKYGGVIINQLKRLGCSCDWDRERFTMDEGLSQAVREV
                     FVRLYNEDMIYQGDYIVNWCPRCHTAISDLEVEFKADQSNLW"
     gene            complement(754..1956)
                     /locus_tag="PLQ77_06350"
     CDS             complement(754..1956)
                     /locus_tag="PLQ77_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10332.1"
                     /translation="MFCRANMKKVLFAIVLFSAVLLIAACGTKARQPESALDTPEHHV
                     FTGMKLIDQEKYQDAEREFALALELNNQYSKAYAGMSLAKAGQQNYTAAFEQLEKAWD
                     TAKTGEEQLAVHIGYIRVNTQSKLACTKIGVDCEQDTSWLKKCESSYQDAVKLDASSA
                     AAPFFMGLAYKTALDYGKAGAMFAKVLEIRKEYVSQANAQWNLVQKIQRAMPGTVTGK
                     KIALLESISRADAAALFMEELKIDVLYKKRTPRTFDTSFKDPEKARSAQTVQPAAKDI
                     AGHPLKADIDGILAIGVRGLEINPQGNFEPAGALNRAEYALMIEDILIKVTGDNVLAT
                     KFIGSTSPFPDLRSDLPYFNAVMVVTSRGIMEAMDITTGEFAPLAPVPGADALLIIRK
                     IKEELKFD"
     gene            complement(2058..3014)
                     /gene="miaA"
                     /locus_tag="PLQ77_06355"
     CDS             complement(2058..3014)
                     /gene="miaA"
                     /locus_tag="PLQ77_06355"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPL10333.1"
                     /translation="MKSNECSSANNSHGIPLLVLGSPTATGKTSLALELAQSLEAEVV
                     NADSLQVYRYLDIGTAKPTRAERERVRHHLLDVVDPDEEYNAALYCEQARTMIGELAG
                     EGRRVLVVGGTGLYIRALLQGIIETPPVDEQIRNYYRDIRDRLGKHHLYGLLEKRDPQ
                     AAGRINPNDAVRVIRALEVLDQSGQSILELQQKHRFADCPYAVFKIGLRVERNELKKR
                     IRQRTEQMMEAGLLDEVRGLLDRGYSETLKPLQSLGYKQMISFIRKRCGLDEAKDSIS
                     RETWQYAKRQMTWFGADRAVTWFGPGETRKIEEKAREFLAQY"
     gene            complement(2983..4776)
                     /gene="mutL"
                     /locus_tag="PLQ77_06360"
     CDS             complement(2983..4776)
                     /gene="mutL"
                     /locus_tag="PLQ77_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPL10334.1"
                     /translation="MSRRIAVLSEEVANQIAAGEVVERPASIVKELVENAVDAGADDI
                     RVELEKGGCRSIRVVDNGSGIEQEDVALVFERHATSKIVRIDDIHDAGSFGFRGEAMA
                     SIASVARVELLTRRPDDLAGTKATAQAGSISEISPAGVPSGTQITVTELFANVPARRK
                     FLKTEATEQSACLDAVMRLALSHPEIRFSVRADGRNMFAAPAAASVADRVAMVLGRDF
                     TDHCLTIAQRKENVELGGMVSTPTWTKSNSKSVYLFVNSRFIRDNSMMHALLAAYRQV
                     MEPRRFPAAVLFLRLPGEEVDINVHPAKMEVRFKNSRIIYDLVSGAVSRALAGAQASR
                     DAVAYRLAPREMAADSSEFQKSRPYQASWGRQRDVFSRANLQRALDEDLFARAGETDV
                     PARVEDASCEERITFDHRTYLGQFAGAYLVFGGPGGLMLLDQHAAHERILLERLKATG
                     KGPVASQPLLMPEVVTLTPAQISLLTEALPLLAGIGLELEVFGRDAVVVKAMPAALPN
                     VDARLLVSDLVDGLGEGQAAPSLAEKKEKILASLACHAAIKAHAELSPAEVAALCRDL
                     EETPFNATCPHGRPIRVRFTLYEIERMFKRK"
     gene            complement(4954..5865)
                     /locus_tag="PLQ77_06365"
     CDS             complement(4954..5865)
                     /locus_tag="PLQ77_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10335.1"
                     /translation="MKLRFVSITVLTGILLIWASAAFCQNAISMSEWVEKVGQGSVNW
                     SAGFIEAKGIGAPPERYQGKINARPLALRAAKVDAYRNLLEITKGVQVDSHTTVRDFT
                     VESDVINTQVEGLVKGAQIVDQQYLSDGTVEVTLRMPLYGELSKIMVPISIQKRKEIP
                     PPAPVVETPAPAPPAPPVAPPPAAPAPPAAVSYTGLVVDARGIQARPAMSPRIYDEDG
                     KEVYGSANVDREYAVQQGMSGYARDLTAAQSNQRVTASPITVKGLKTGGAGRSDVVIS
                     NADAQLIRASSENASFMKKCRVMIVLD"
     gene            6127..8916
                     /gene="ileS"
                     /locus_tag="PLQ77_06370"
     CDS             6127..8916
                     /gene="ileS"
                     /locus_tag="PLQ77_06370"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPL10336.1"
                     /translation="MDYKTTLNLPQTDFPMKANLAVREPEMLKKWEHMDIYHKIRKEA
                     KGRKPYILHDGPPYANGNIHLGTALNKIIKDIVIKAKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGGKDSGLSQVEKRRACRVYAEKYVGIQRDQFKRLGVFGEWENPYLTMAYPY
                     EAATVHEFGKLFLNGSVYKGKKPVYWCATCKTALAEAEVEYADHHTPSIYVRFAFTSD
                     IGAIRPKLAGKKVFMVIWTTTPWTIPANLAIAVKDDFIYAAVKIDDDVLIVAKDMLDY
                     CLDAFGHRGKACEIVDEFKGEVLEGQKCVHPLIQRNSLLILAPFVTLEAGTGAVHIAP
                     GHGQEDYEIGLEYGLENYAPVDDAGKFTEDVEHFAGQFVFDANDNVIQKLKEAGALLG
                     HVPMQHSYPHCWRCKKPIIFRSTEQWFISMEKNDLRKKALAAINEVQWIPSWGRDRIY
                     GMVENRPDWCISRQRLWGVPITIFYCAKCKNELLTQDILNHLVALVEKNGADVWFEKE
                     PKDLMPKHTVCPHCRSTEFTKEVNILDVWFDSGVSHAAVLEKRPELGSPCDLYLEGSD
                     QHRGWFHSSLLESVGTRGRAPYKSVLTHGFVVDGEGKKMSKSGGNAIGAEEAVSKYGA
                     EILRLWVAAEDYTDDIRISEEILKRLMEAYRRIRNTSRFILGNLYDFAPDADRLDGDQ
                     MLEMDRWALHRLQEVIRRVTEAYERHQFHMVFYTLYNYCTVDLSALYLDVLKDRLYTS
                     KTASAARRSGQTAMFIILNAMTRLLAPILTFTAEEVWAALPAWNGKEESVHLTQFPQV
                     EEKYFNASLGERWKAMIDAKSEIAKAVEQARKEKVIGHSLDARISIAAPDKMKDLFAA
                     HLEDLRALLIVSQLQLVDESAIANPYKSEEIKGMAVGVEKARGAKCERCWIYEESVGT
                     HADHPTVCARCLSNL"
     gene            9093..>9420
                     /locus_tag="PLQ77_06375"
     CDS             9093..>9420
                     /locus_tag="PLQ77_06375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPL10337.1"
                     /translation="MKKNLLMFILGAAVIIALDQITKSAVLARFSLHETLEVIPGFFN
                     LVYVMNPGAAFGFLADASATFRYVFFIGITIAAAGLIVYYLVKSNLRNMLLAVSLTLI
                     FGGAVGN"
BASE COUNT         2011 a         2735 c         2598 g         2076 t
ORIGIN      
        1 ttccacagat tgctctggtc ggccttgaat tccacttcca gatcggaaat ggcggtgtgg
       61 cagcgcggac accagttgac gatgtagtcg ccctgataga tcatgtcttc gttgtagagg
      121 cggacaaaaa cctcgcggac cgcctgggaa agaccctcgt ccatggtgaa gcgctcgcgg
      181 tcccagtcgc aggaacagcc caggcgcttg agctggttga tgatgacgcc gccgtatttt
      241 tccttccatt cccagacgcg ggcgatgaat ttttcgcggc ccagatcgtg acgggagagc
      301 ccctccttgc ggagctgctg ctccacgacg ttttgcgtcg cgatgcccgc atggtccatg
      361 cccggcatcc aaagcgtgtt gtagccctgc attcttttga agcggatgat gatgtcctga
      421 agggtattgt tgagcgcgtg gcccatgtgc agcattccgg tcacgttggg aggcggaatg
      481 acgatggaaa aaggcggcgc ttcgctggtg tcctgcgcgt ggaaaaactt attctcgagc
      541 cagtaatcgt acagtctttt ttcggcctcg gccggttcaa atgccttgtc caactctttg
      601 attgtcatgt aagtcttact ctcctgtctg cgcgcaatcg caaagggcct gcgccctttg
      661 cggacggact ttttcgccac gtccgtcctc gcgtaaaatt tgaggcggga aaaaaccctt
      721 cccgcctcgt ggatgtgtca acatcatttt cttttaatcg aatttcagct cttccttgat
      781 cttgcggatg atcagaagag cgtccgctcc gggaacgggc gcaagcgggg cgaactcccc
      841 ggtcgtgatg tccatcgcct ccatgatgcc ccgggaggtc accaccatga ccgcgttgaa
      901 atacggcagg tcagaccgga gatcaggaaa gggcgaggtc gatccgataa acttggtggc
      961 cagcacgttg tcacccgtca ctttaattaa aatgtcttcg atcatcaggg cgtactcggc
     1021 gcggttcagg gcgccggccg gctcgaaatt gccctgcgga ttgatttcca ggccgcgaac
     1081 gccgatcgcc aggatcccat cgatatccgc cttcagggga tggccggcaa tgtctttggc
     1141 ggcaggctgt accgtctggg cgctccgggc tttttcggga tccttgaagg acgtgtcaaa
     1201 tgttctgggc gttctctttt tatacagaac gtcgattttt aactcctcca tgaacagagc
     1261 cgcggcatcg gcgcgggaaa tgctctccag aagggcaatc tttttgcccg tcactgttcc
     1321 gggcatggcc cgctggattt tctgaaccag gttccactgg gcattggcct ggctgacata
     1381 ttctttgcgg atttcaagaa cttttgcaaa catggcgcct gccttgccgt agtcgagggc
     1441 cgtcttgtac gccagcccca tgaaaaacgg agcggcggcg gaagaagcgt ccagcttcac
     1501 cgcgtcctga tacgagcttt cgcacttttt cagccagctt gtgtcctgct cgcagtccac
     1561 gccgattttc gtgcaggcaa gcttgctctg cgtattcacg cggatataac cgatgtgaac
     1621 ggcaagctgt tcttcgccgg tctttgccgt atcccacgcc ttctccagtt gttcaaaggc
     1681 ggcggtataa ttctgctgac cggctttcgc caggctcatt ccggcatagg ctttggaata
     1741 ttgattgttc agttccagcg ccagtgcaaa ttcccgttct gcgtcctgat atttttcctg
     1801 atcaatcagt ttcatgccgg taaagacatg atgctccgga gtgtcgagcg cgctttccgg
     1861 ctgacgggcc ttcgtcccgc atgcggcaat cagcaaaaca gccgaaaaca aaacgatcgc
     1921 aaagagaacc tttttcatgt tcgcgcggca aaacatacat tcgctccttt ctgcaaaagg
     1981 gttattccct gaaaattcct tctgtttcct tcccaaaaag ctttgtatca ttagcttcag
     2041 tgccttgtga agtcaagtca atattgcgcc agaaattccc gcgctttttc ttctattttt
     2101 cgtgtctcgc cgggtccgaa ccacgtgacg gccctgtccg cgccgaacca tgtcatctgc
     2161 cgtttggcat attgccaggt ctcgcggctg atagagtctt ttgcctcatc cagaccgcat
     2221 cgtttccgaa taaaagaaat catctgcttg tatccgaggg actgaagggg tttgagcgtt
     2281 tctgaatagc cccggtccag aagcccccgc acctcatcga gcagccccgc ttccatcatt
     2341 tgttcggttc tttggcggat tctctttttc aattcgtttc gttccacgcg aaggccgatc
     2401 ttgaagacgg catacggaca atcggcaaac cggtgctttt gctgcagttc cagtatggat
     2461 tggccgctct ggtccagcac ttccagcgcc cgaatcaccc gtacggcatc gttgggattg
     2521 atgcggccgg ccgcctgcgg gtcccgtttt tcaagaaggc cgtaaagatg atgctttcca
     2581 agtctgtcac gaatatcccg gtaataattg cgaatttgct catcgaccgg cggcgtttcg
     2641 ataatgccct gcaaaagcgc gcggatatag aggcccgttc ccccgaccac cagcacccgc
     2701 ctgccctcac ccgccagttc cccgatcatt gttctggcct gttcgcaata cagagccgca
     2761 ttgtattcct cgtccgggtc caccacgtcc agcaggtggt gcctgaccct ttccctttcc
     2821 gcccgtgtcg gtttggccgt gccgatatcc agataacgat agacctgcag cgaatccgcg
     2881 ttgacaacct cggcttccag ggactgggcc agctccagcg cgaggctggt tttccctgtt
     2941 gccgtaggcg aacccaggac cagcagggga atcccgtgcg aattatttgc gcttgaacat
     3001 tcgttcgatt tcataaagcg taaaccggac acgaatcggg cgtccgtgcg gacaggtggc
     3061 gttaaagggt gtttcttcca ggtcgcggca caaagcggca acctccgcgg gagaaagttc
     3121 cgcgtgggcc ttgatcgctg catggcaggc cagggacgcc aggattttct cctttttctc
     3181 cgcaagcgaa ggggctgcct gtccttcgcc cagcccgtca accagatcgg ataccagcag
     3241 gcgcgcatca acattcggca gggcggcggg catggccttc accacgaccg cgtcgcggcc
     3301 gaagacctcc agctccaaac cgatgccggc aagcagcggc agcgcttccg tgagaaggct
     3361 gatctgcgcg ggagtcagcg ttaccacttc cggcatcaac aggggctggc ttgcaactgg
     3421 tcctttcccg gttgccttca ggcgctccag aagaattctt tcatgcgccg cgtgctgatc
     3481 cagcagcatc aggccgcccg ggcctccaaa cacgagatag gcgccggcaa actgccccag
     3541 ataggttcga tggtcaaacg tgattcgttc ttcgcaggaa gcatcctcca cgcgggcggg
     3601 aacatccgtt tccccggcac gcgcgaacaa atcctcatca agagccctct gaaggtttgc
     3661 ccgcgaaaac acgtccctct gccttcccca tgaggcctga taaggcctgg atttttgaaa
     3721 ttccgaagag tcggcagcca tctcacgcgg cgccagacga taggccacgg catccctgga
     3781 agcctgcgct ccggccaggg ctctcgaaac cgccccggac accaaatcat aaatgatgcg
     3841 ggaatttttg aagcgaactt ccattttggc cgggtgcacg ttgatgtcca cctcctctcc
     3901 cggaagacgc agaaacagga ccgccgcggg aaagcgcctg ggctccatca cctggcggta
     3961 ggccgcgagc agcgcgtgca tcatggaatt gtcgcggata aaacgggaat tgacaaacag
     4021 ataaacgctt ttggaattgg atttcgtcca tgtcggcgtg gaaaccatgc cgccaagttc
     4081 gacgttttcc tttcgctggg cgatcgtcag gcagtgatcc gtaaagtccc tgcccagaac
     4141 catggcgacg cgatccgcga cgctcgccgc cgccggcgcc gcgaacatgt ttcgtccgtc
     4201 cgcgcggacg gaaaagcgga tctccgggtg tgaaagcgcc agccgcatga ccgcatccag
     4261 gcaggcgctc tgctcggtgg cttctgtctt gagaaacttc cggcgcgccg gcacattggc
     4321 aaagagctcc gtcacggtta tttgcgttcc ggagggcacg cccgccggag atatttcgga
     4381 aatcgagcct gcctgcgctg tcgctttcgt tccggccaga tcgtcgggac ggcgggtcag
     4441 aagctccacg cgggcgacgg aggcgatgct ggccatcgcc tcgccccgaa agccgaagga
     4501 gccggcatca tggatatcgt cgatccggac gattttgctg gtggcgtggc gttcaaaaac
     4561 cagcgcgacg tcttcctgtt caatgccgga tccgttgtca acgacccgaa tagagcggca
     4621 tccgcctttt tccagttcga cgcgaatgtc atcagccccc gcatcgacgg cgttttccac
     4681 cagttccttg acgattgacg ccggcctttc caccacttct cctgcggcaa tctgattggc
     4741 gacttcttcg gataaaacgg caattcgacg ggacaagatc catctcctgc aaattatttt
     4801 gccgcggcca catgatgatt ggacgcggtt catcaaatcc gcggtttgcg gtccatcgcg
     4861 gcggctgagc cttcaggatg gtttccaaga aaaaagccgc agccaccaaa accccgtgac
     4921 tgcggccctg ttgcgcaatt ttacgcaagt cctctaatcc agaacgatca tgacgcggca
     4981 ttttttcatg aaagacgcgt tttccgacga agcgcgaatg agctgggcgt cggcattgct
     5041 gatcacgaca tcggaccggc ccgcgccgcc ggtctttaat cctttaaccg taatcgggct
     5101 ggccgtgacg cgctgattgc tttgcgcggc ggtgaggtcg cgggcatacc cgctcatccc
     5161 ctgctgaacg gcgtattcgc ggtccacatt ggccgaaccg tacacttcct tgccgtcctc
     5221 gtcgtaaatg cgcggcgaca tggccggacg cgcctgaatg ccgcgtgcgt caaccaccag
     5281 tccggtatag gaaacagcgg ccggaggcgc tggagccgcg ggcggcggcg caacgggagg
     5341 cgcgggcggt gcgggagccg gcgtttcgac gacgggagcc ggaggcggaa tttctttgcg
     5401 tttctgaatg gaaatgggaa ccatgatttt ggacaattcc ccgtacagcg gcattctcag
     5461 cgtcacttcg acggtgccgt cagacaaata ctgctggtca acgatctgcg cccctttgac
     5521 caacccttcg acctgcgtgt tgatgacgtc gctctcaacg gtaaagtccc tcaccgtcgt
     5581 gtgcgaatcc acttgaacgc ccttggtgat ttccaggaga ttccggtagg cgtccacttt
     5641 cgcggcgcgc agcgccaggg gacgggcatt gatttttccc tggtatcttt ccggcggcgc
     5701 gccgatgcct tttgcttcaa taaagcccgc cgaccagttt accgaaccct ggccgacttt
     5761 ttccacccac tcggacatgc tgatcgcatt ctgacaaaaa gcggcggatg cccatattaa
     5821 caggataccc gtcaaaacgg tgatggaaac gaatctcaac ttcatattgt cctccttgca
     5881 tggttggcgt cctgaaaaat tttcctcttc gaaaccggca caaaactaac aaaatattct
     5941 ttcgcttgca ataagaattc acagcctgtc cggcaaatga aaacaccgct tccttcggca
     6001 agagttgtgc ttgaaactcc acggtttatg tgatacttga gcgtgcggat aaaggctgca
     6061 tacaagcaga tgaaatcatt cgcggtttaa ccgctatttt tttgattgga caggagcgac
     6121 gacaatatgg attacaaaac gacccttaat cttccccaaa ccgatttccc catgaaggct
     6181 aacctggccg tgcgcgagcc ggaaatgctg aaaaaatggg aacatatgga catttaccac
     6241 aagatccgga aggaagcgaa aggcagaaaa ccctatattc tgcatgacgg ccctccctac
     6301 gccaacggca atattcacct gggaacggcg ctgaacaaaa tcatcaagga tatcgtgatc
     6361 aaagccaaaa acatgaccgg ctttgacggc gtgtacgtcc cgggctggga ctgccacggc
     6421 ctgccgatcg aacaccaggt ggacaaggaa ctgggcggaa aagactccgg cctgtcccag
     6481 gtggaaaaac gccgcgcctg ccgcgtgtac gcggaaaagt atgtcggcat tcagcgcgat
     6541 caattcaagc gcctcggcgt tttcggcgaa tgggaaaacc cctatctgac catggcctat
     6601 ccgtatgaag ccgccacggt ccatgagttc ggcaaactgt ttctgaacgg cagcgtttat
     6661 aaggggaaaa agcccgtgta ctggtgcgcc acctgcaaga cggcgctggc ggaggcggaa
     6721 gtggaatatg ccgaccatca taccccttcc atttatgtcc ggtttgcctt cacatcggac
     6781 atcggggcca ttcggcccaa actggcgggg aagaaagttt tcatggtgat ctggacgacc
     6841 acgccctgga cgattcccgc caacctggcc atcgccgtca aggacgattt catctacgcc
     6901 gccgtgaaaa tcgacgacga cgtcctgatc gtagccaaag acatgctgga ttattgcctg
     6961 gacgctttcg ggcaccgcgg caaagcctgt gagatcgtcg atgaattcaa gggcgaagtg
     7021 ctggaaggac aaaagtgcgt ccatcccctg attcagagaa acagcctgct cattctggct
     7081 ccctttgtca cgctggaagc cggcacgggc gccgtgcaca tcgctcccgg ccacggccag
     7141 gaagactatg aaatcggctt ggaatatgga ctagaaaact acgcgccggt ggatgacgcg
     7201 ggaaaattca cggaagatgt cgaacatttt gccggtcagt tcgtgtttga cgcaaacgat
     7261 aacgtcattc agaagctgaa agaagctggc gcgcttttgg gacacgttcc catgcagcat
     7321 tcctacccgc actgctggcg ctgcaaaaag cccatcatct tccgttcgac cgagcagtgg
     7381 tttatctcca tggagaaaaa cgacctgcgc aagaaggcgc tggcggccat caacgaggtg
     7441 cagtggatcc cctcctgggg gcgcgaccgc atctacggca tggtggaaaa ccgcccggac
     7501 tggtgcattt cccgccagcg gctgtggggc gttccgatca cgattttcta ctgcgccaaa
     7561 tgcaaaaacg aactgctgac gcaggacatc ctcaaccacc tggtggcgct ggtggaaaaa
     7621 aacggcgcgg atgtctggtt tgaaaaagag ccgaaagacc tgatgcccaa acacaccgtc
     7681 tgccctcact gccggtcaac ggagttcacc aaggaggtca atattctgga tgtctggttt
     7741 gattcgggcg tcagccacgc ggcggtcctg gaaaaaaggc cggagctggg ctctccctgc
     7801 gacctgtatc tggaaggctc cgatcagcat cggggctggt tccactcctc ccttctggaa
     7861 tccgtgggaa cccgcggacg ggccccctac aaaagcgtcc tgacccacgg ctttgtcgtg
     7921 gacggcgaag ggaaaaagat gtccaaatcc ggcggcaacg ccatcggcgc ggaagaagcc
     7981 gtttccaaat acggcgcgga gatcctgcgg ctctgggtgg cggcggagga ctacaccgac
     8041 gacatccgca tctccgagga aattctcaag cgcctgatgg aagcctaccg gcgcatccgc
     8101 aacaccagcc ggttcatcct gggcaatctg tatgactttg caccggatgc cgaccgtctg
     8161 gatggcgacc aaatgctgga aatggaccgg tgggccctgc accgcctgca ggaagtgatc
     8221 aggcgcgtga cggaggccta tgaacgccac cagttccaca tggttttcta tacgctctac
     8281 aattactgca cggtggattt gagcgcgctg tacctggacg ttttgaagga ccgcctctat
     8341 accagcaaaa ccgcctccgc cgcccggcgt tcgggccaga cggcgatgtt catcatttta
     8401 aacgcgatga cccgcctgct cgctccgatc cttacgttca ccgcggaaga ggtctgggcg
     8461 gccctgcccg cctggaacgg caaagaagaa agcgtccatc tgacgcagtt tccgcaggtg
     8521 gaagaaaagt atttcaacgc ttctctgggc gagcgctgga aagccatgat tgacgccaaa
     8581 agcgaaatcg ccaaagccgt cgaacaggcg cgcaaggaaa aagtgatcgg ccattcgctg
     8641 gatgcacgaa tcagcatcgc cgcgccggac aaaatgaaag atctgtttgc cgcgcatctg
     8701 gaagacctgc gcgcgcttct gattgtttcg caactgcagc tggtggatga atccgccatt
     8761 gccaatcctt acaaaagcga ggaaatcaag ggaatggcgg tcggcgtgga aaaggcccgc
     8821 ggcgccaaat gcgaacgctg ctggatttat gaagaatcgg tcggaaccca tgcggatcat
     8881 cccacggttt gcgcccgctg cctgtcgaat ctgtagagaa cgggtcctgg gtgatgggaa
     8941 aagtgaacgt agggcggacc ttcaggtccg cctaatgcgg gtctaaagac ccgcgctgca
     9001 ttcctgtaaa aaactgaaga gtgacgagtg aaacgtaggg cgggccttta gacccgccat
     9061 cactatagcc atacaggcaa caaggagttt ttttgaagaa aaatctttta atgttcattc
     9121 tgggcgcggc ggtcatcatc gcgctggatc aaattacaaa atcagccgtc ctggcgcgtt
     9181 tttctcttca tgaaacgctg gaagtcattc ccggtttttt caacctggtc tatgtcatga
     9241 atcccggcgc ggccttcgga tttctggccg atgcctccgc gaccttccgt tatgtattct
     9301 tcatcggcat cacgattgcg gccgccggtt tgattgttta ttatctcgtg aaaagcaatc
     9361 tgcgaaacat gctgctggcc gtttccctga ccttgatttt cggcggcgcc gtcggaaatc
//
LOCUS       DAOPAF010000061         9398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6922_length_9398_cov_44.752863, whole genome shotgun sequence.
ACCESSION   DAOPAF010000061 DAOPAF010000000
VERSION     DAOPAF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_9398_cov_44.752863"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLQ77_06380"
     CDS             <1..479
                     /locus_tag="PLQ77_06380"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPL10338.1"
                     /translation="AAAKKVASIGIPCIIAPGKKKKVLSDIMAGREIGTLFLPQTAKL
                     ASRKYWIAFTLRPRGRLILDDGAKKALLEKGKSLLPSGITGVEGEFDPGDPVACVDRD
                     GNLLAKGLVNYSDGDVRRILGLKTAQIFSVLGHKDYDEVIHRDNLVVTGDLKKNKG"
     gene            542..1135
                     /locus_tag="PLQ77_06385"
     CDS             542..1135
                     /locus_tag="PLQ77_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPL10339.1"
                     /translation="MIPLIVLVVIIAAAAFYGVGIYNHLITLRNLYRNAFTQIDIQLK
                     RRYDLIPNLVEAAKGYLAHERGTLEAVMKARNSAVDASKAAAQSPGSAAAMTGLSQAE
                     GALAGVLSRLLAVVEAYPDLKANQTIADLMEELTSTENKIGFARQAYNDAVTGYNIQR
                     EKFPQNVIASAFDFKAAALLDAVEKPEERTAPKVSFS"
     gene            1144..3132
                     /locus_tag="PLQ77_06390"
     CDS             1144..3132
                     /locus_tag="PLQ77_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPL10340.1"
                     /translation="MNTDRSTNFFARQARARKNGRRQLIAFFFAVAVIVLVTTLAIRL
                     AWYLYVGTNAYTSFNAGEAHDYHQRLSTFTFFDPAFFIFMSMAIVIVILAASIYKMHT
                     LKKGGGAVAEMLGASRIDSKTTDPAKRRLINVVEEMAIASGIPVPQVFVLDSEPNINA
                     FAAGLELSDAAVTVTRGALTRLNRDELQGVVAHEFSHILNGDMRLNVQLIGILFGILF
                     LGIAGQKFLSGGRVSLRAGLPAMAAGIFLVIIGQAGSFLGRLMQCAIARQQEFLADAS
                     AVQFTRNPPGLAGALKKIGGGAFGSRIGSAKARQASHLFFSESHPASWFSFLDTHPPL
                     ITRIRLLDPSFDGKFPRMADEPIAPKPIYTDPFGGTGHYRRPSVRGASALAADVTSLV
                     GNPLPGHLEESRTILDALPQDVREMAATPRGAASVVFALLIGRDASRREQQAAALNRT
                     SVLQGDTEMVFSLGDQLAGLSNSLKLPLLELAMPSLSVMTGMEKRNFLLMLHSLIHAD
                     GKLSLLELSVQWILEKHLNPSEDLFRTIRKFSFSQVGLDIVTLLSALASAGHTHDKDK
                     AQKAFDAGVARIPELAARKPVLSFQEIMSYAAVNRALQSLTDASFKIKESVVDACAHC
                     AFSDQAVTFEEGELLRVVSLALQCPLPPFIQPPLRDAA"
     gene            3204..4541
                     /locus_tag="PLQ77_06395"
     CDS             3204..4541
                     /locus_tag="PLQ77_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPL10341.1"
                     /translation="MSIWQKKSRILITCPKGVVPYLKAEIEALGFPVVQEIDTAVSTE
                     GTLADTMSLNLHLRTAQHVLYQLQIFKALSPGALYERLNAIPWETILHDSGPSAYVCV
                     TSVADHPDVTDSRYVNLRAKDAIVDRIREKTGIRPDSGPERNRAVIHVYWRNDQAMAY
                     IDTSGERLSLRGYRKIPLAAPMQETLAAALIMATQWRGRTPLVNPMCGSGTLAIEAAL
                     IALHRAAGLGRSNYGFLHIRDFPREAWQALRKDARDASLKALPSTIIASDIDPRAVEA
                     ARRNAQTAGVDRLIEFRVCPFEKTPTPGGKGVIVMNPPYGERLEPFKIKTSHPEHRKE
                     IAPGQKVIVRKAADIVHRSGGSTTLQRLEALYEGCGDWFKSIGRGYLGYIFTGNLEMA
                     KKVGLRTRRRMTFYNGEIECRLLEYELYAGSKKHKAAPARPDDADTGAQHGSG"
     gene            complement(4547..4936)
                     /locus_tag="PLQ77_06400"
     CDS             complement(4547..4936)
                     /locus_tag="PLQ77_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10342.1"
                     /translation="MTDILDYISTWSALKWVVLVLIAGFIGQFGRMTAEAITEKIRLR
                     RAGKNPLTHGPQNPEEPKALSTGAVGENGLPVPVQEMKNLQAQVPVAETPPAAASPVF
                     PPDKKALKAMAKARKKEAKKKEKESLP"
     gene            5132..6223
                     /locus_tag="PLQ77_06405"
     CDS             5132..6223
                     /locus_tag="PLQ77_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPL10343.1"
                     /translation="MRKAIVKSITLLLIACVSACAPPPLQKKDDKIAAALADPQTSFF
                     EACRRGDIAMVMRLAEEKRIDVNQADDYAWTGLMYAVQNGHTGLAEYFLLQKSQINRQ
                     DGDGLTPLMIAANERHSDMVDLLLAFGADPLLQDRHGYTALMWAIYNKSWDCIPPLLA
                     EGKGLDVQNGFGETALHLALDYHCSDIAGILIRQNANVKFADGRGRTPLHLAAERGDE
                     DNAWLLLSRGADANAGDESGENPLMKSIQSGNPDLALLLLPRTGNLQATNREGRTALH
                     LAVVRDLPDTAAALIRRKADLNSSDEQGFTPLMLAAESGSERMTVLLAERGADLKMKN
                     KEGKTALDLAREKSHGNIATYLERKMGKP"
     gene            6220..7515
                     /locus_tag="PLQ77_06410"
     CDS             6220..7515
                     /locus_tag="PLQ77_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL10344.1"
                     /translation="MKEQPDLFRWMLAAALTIVLIAGLISCRYLSIPAQDDAVPALLP
                     SKEEKGPTVYNSAVIKGRVTGPGWKGHSTLILACRSADKKNGCGEYAATRENQDAFML
                     YLPEGRYCLIAVTDHNHDGKYEKDEASGAYGLPRQMMEITVREGDLITGVEIRTAAAG
                     ERPVRLPRECRWKIEEPVIRQATGNGDLLKIYHEYFSLENAQTGYWNPSSFMKAFGAH
                     IYLTQAYDSRKIPLLFVHGTEGTPQNWIYCYMRLDRDRYQPWFFYYPSGIRLNLAAAL
                     LQEELRELQEQYGFRKMALAAHSVGGLTARSFLIRRESEERPPFIRLFASFATPWNGF
                     GLADASRVLPHKSIPVWLDLGTHSEFISATMNAKLPPSVRHYLFYGREDKLTGGAEPD
                     SRATAGTAGVYGFDCTHDGILIDRRVFSRFNTILEKEMN"
     gene            7517..8323
                     /locus_tag="PLQ77_06415"
     CDS             7517..8323
                     /locus_tag="PLQ77_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPL10345.1"
                     /translation="MKIIRFLSESGKTLYGAYSKRRPGTAEGIEKEPWSKNITGGGEE
                     KIRKLLAPVAPVNILALGINYRKHGEETALSIPSEPILFLKATSSVTGPDHPIVLPAA
                     GSDQVDYEAELALVIGKTAKNVTPAEAWDCIFGFTCANDVSARDWQFERQKGQWARGK
                     SFDTFCPLGPWIVTKDEIDDPDDLAIRAVLNGRTVQESRTSEMIFNVRTIVSRLSRSM
                     TLHPGTVILTGTPEGVGFARRPPVFLKPGDVIRVEIEKIGTLSNRVIAES"
     gene            8320..8634
                     /locus_tag="PLQ77_06420"
     CDS             8320..8634
                     /locus_tag="PLQ77_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10346.1"
                     /translation="MKIIRTRWVPRRKFAAMNLFGIVFAPKGQILPPRTINHEAIHTA
                     QMREMLYIFFYLWYFLEWLFRLRRKTSAYRRIGFEREAYHHEHDLEYLKSRKPYQWIR
                     YL"
     gene            8667..9239
                     /locus_tag="PLQ77_06425"
     CDS             8667..9239
                     /locus_tag="PLQ77_06425"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPL10347.1"
                     /translation="MQTSWKNGKEKIYTRAIDISTWEYDAERIVVEGFLRDDRYQDTH
                     TVTGETFPRGTLHHMSIRLLVNCSTLVIEDLDMELLSVPREVCRETMGFLAPVKGMTI
                     ARGFTARIKKLVGGPKGCAHVVELLLAMAPAVLQGVAAARSRRPSGVDDGQSKIILQY
                     LINTCHAWREDGPFVEAYAQKIKAMTKSAL"
     gene            complement(9309..>9398)
                     /gene="rrf"
                     /locus_tag="PLQ77_06430"
     rRNA            complement(9309..>9398)
                     /gene="rrf"
                     /locus_tag="PLQ77_06430"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2054 a         2850 c         2671 g         1823 t
ORIGIN      
        1 tcgccgccgc caaaaaagtc gcctccatcg gcattccctg catcatcgcg ccgggaaaga
       61 aaaagaaagt gctgtccgac atcatggcgg gccgggaaat cggcacgctg tttctgccgc
      121 agaccgcgaa actggccagc cgcaagtact ggatcgcctt cacgctgcgc ccccgcggac
      181 gcctgattct cgacgacggc gccaagaaag ccctgctcga aaaaggcaaa agcctgctgc
      241 cgtcgggcat caccggcgtg gaaggggaat tcgatccggg ggatcccgtc gcctgcgtgg
      301 accgcgatgg aaacctgctg gccaaggggc tcgtcaatta cagcgacggc gatgtccgcc
      361 ggatactggg actgaagacc gcacagattt tttccgtgct gggccacaag gactatgacg
      421 aggtgatcca ccgcgacaat ctggtcgtca ccggcgacct caagaaaaac aaaggatgat
      481 ccgataaatc cgatcagcgc cagaaatgcg cccgtccggg cgcaacgtta aggaggttaa
      541 catgattcca ttgatcgttc tggtcgtcat catcgccgcc gccgcgtttt atggtgtcgg
      601 aatttacaat cacctgatca cgctgcgcaa tctttaccgg aacgccttca cccagatcga
      661 cattcagctc aagcgccgct acgatctgat tcccaacctg gtggaggcgg ccaaaggcta
      721 cctggcgcac gaacgcggca cgctcgaagc cgtcatgaaa gcgcgaaatt ccgccgtgga
      781 cgcaagcaaa gcggccgccc agtctcccgg cagcgccgcg gccatgacgg gcttgagcca
      841 ggcggaaggg gcgcttgccg gagttctttc caggctgctg gcggtggttg aagcctaccc
      901 ggatctgaaa gccaaccaga ccatcgccga tctgatggag gagctgacct cgacggaaaa
      961 caagatcggc ttcgcgcggc aggcttacaa cgacgccgtc accggctaca acattcagcg
     1021 ggaaaaattt ccccagaacg tgatcgcgtc cgcgtttgat ttcaaggccg ccgcccttct
     1081 ggacgccgtg gaaaaacccg aagagcggac ggcgcccaaa gtgtctttct cctgacggaa
     1141 agcatgaaca ccgaccgttc gaccaacttc tttgccaggc aggccagggc ccgcaaaaac
     1201 ggccggcggc agctcatcgc gtttttcttc gccgtggccg taattgtgct ggtgacgacc
     1261 ctggccatcc gcctggcctg gtacctgtat gtcggcacaa acgcctacac ctccttcaac
     1321 gccggcgaag cccacgacta ccaccagagg ctttccacct tcacgttttt cgatcccgcg
     1381 tttttcatct tcatgtccat ggccattgtc atcgtgatcc tggccgccag catctacaag
     1441 atgcacactc tgaaaaaagg cggcggcgcc gtggcggaaa tgctgggcgc cagccggatc
     1501 gactccaaaa caacggatcc ggccaaacgc cgtctgatca acgtggtgga ggaaatggcc
     1561 atcgcctcgg gcatcccagt cccgcaggtc tttgtcctgg attccgaacc caacatcaac
     1621 gcctttgccg ccggactgga attgagcgac gcggcggtaa ccgtcacgcg cggggcgctc
     1681 accaggctca accgcgacga actgcagggc gtcgtcgccc acgagttcag ccacattctc
     1741 aacggcgaca tgcgcctcaa cgtccagctc atcggcatcc tcttcggcat tcttttcctg
     1801 ggcattgccg gacaaaaatt tctctccggc ggaagagtat cgttgcgggc ggggcttccg
     1861 gcgatggccg ccgggatttt cctggtcatc atcgggcagg ccggctcgtt tctaggccgc
     1921 ctgatgcaat gcgccatcgc ccgtcagcag gaatttctgg cggacgcctc cgcggtgcag
     1981 ttcacgcgca atccgccggg gttggcgggc gcgctcaaaa aaatcggcgg cggcgccttc
     2041 ggttcgcgca tcggatcggc caaggcgcgg caggccagcc atttattttt cagcgaaagc
     2101 catcccgcaa gctggttttc ctttctggac acgcatccgc cgctcatcac ccgcatccgc
     2161 ctgctggatc cttcctttga cggcaagttc ccccgaatgg ccgacgaacc gatcgccccc
     2221 aaacccatct acacggaccc gttcggggga accgggcatt atcgccggcc ctccgttcga
     2281 ggtgcatcgg cactggcggc ggacgttacc tctctggtcg gcaacccttt gcccggccat
     2341 cttgaagaaa gtcggaccat tttggacgcc ctgccgcagg acgtccgcga gatggccgcg
     2401 acacccaggg gcgcagcctc cgtcgttttc gcgctcctca tcggcaggga tgcctcacgc
     2461 cgtgaacagc aggccgctgc gctgaaccga acatcggtcc tgcagggaga cacggaaatg
     2521 gttttctctc tgggcgatca gttggccggg ttgtccaaca gcctgaagct gcctcttctg
     2581 gaactggcca tgccgtcttt gtcggtcatg accggcatgg aaaaacgaaa tttcctgctg
     2641 atgctgcact ccctgatcca tgccgacggc aaactgagcc tgctggagct gtccgttcaa
     2701 tggattctgg aaaagcattt aaacccttcc gaagacctgt ttcgcaccat caggaaattt
     2761 tccttctccc aggtggggct ggacattgta acgcttcttt ccgccctggc ctccgccgga
     2821 cacacgcacg ataaagacaa agcgcaaaag gcgtttgacg caggtgtggc cagaatcccg
     2881 gagctggccg cccggaaacc ggttctttcg tttcaagaaa tcatgtccta cgctgccgtg
     2941 aaccgggcgc tccaatcgct taccgatgcc tccttcaaga tcaaggaatc cgtcgtcgat
     3001 gcctgcgcgc actgcgcctt ttcggatcaa gccgtcacgt tcgaggaggg tgaactcctg
     3061 cgcgtcgtct cgctggcgct gcaatgcccc ctcccgccgt ttatccagcc gccgctgcgg
     3121 gatgccgcct gattcgaaga agtttcgaaa acctgaattt ttaaaagatt gccaagacct
     3181 atgggctgtg ttaaagcctg cgcatgtcca tctggcaaaa gaaaagcagg atattgatca
     3241 cctgtccgaa aggggttgtt ccttacctga aggcggaaat cgaggcgctg ggatttcccg
     3301 tcgtgcagga aatcgacacg gccgtttcca ccgagggaac cctggcggat acgatgtctt
     3361 taaacctgca cctgcgcacg gcccagcacg ttttgtatca actgcaaatc ttcaaggccc
     3421 tctcccccgg cgctctttac gaacgactga acgccattcc ctgggaaacg attctgcacg
     3481 actccggccc cagcgcctat gtttgcgtca catccgtggc cgatcacccg gacgttaccg
     3541 attcgcgcta cgtcaacctc agggccaagg acgccattgt tgaccgaatc cgcgaaaaga
     3601 cgggcattcg gccggattcc gggccggaga gaaacagggc ggtcattcat gtttactggc
     3661 gaaacgatca ggccatggca tacattgaca cgtcggggga aagactttcc cttcgcggct
     3721 accgcaagat tccgctggcg gcgccgatgc aggaaacgct ggcggcggcg ctcatcatgg
     3781 ccacccaatg gcggggccga acgccgcttg tcaatccgat gtgcggcagc ggcacccttg
     3841 caatcgaggc ggccctgatc gcgctccatc gggcggcggg actggggcgc agcaattacg
     3901 gcttcctgca tatccgggat ttcccgcggg aagcgtggca ggcgctgcgc aaagacgcac
     3961 gcgacgcgtc attgaaagct ttgccgtcca ccattatcgc atcggacatc gatccccggg
     4021 cggtggaggc cgcgcgccga aacgcgcaaa cggccggggt tgaccgcctc atcgaattca
     4081 gggtctgtcc ctttgaaaaa acacccacgc cgggcggcaa aggcgttatc gtgatgaatc
     4141 ccccctacgg agaacggctg gagcctttta aaatcaaaac gtctcatccg gagcacagga
     4201 aggaaatcgc gccggggcag aaagtcatcg tccgcaaggc cgccgacatc gttcaccgaa
     4261 gcggcggttc caccaccctt cagcgcctgg aagcgcttta cgaaggctgc ggcgactggt
     4321 tcaaaagcat cggccggggt tatctcggct atatttttac gggcaatctg gagatggcca
     4381 aaaaagtggg gctgcgaacc aggcgccgaa tgacttttta caacggggaa atcgaatgcc
     4441 gtctgctgga gtatgaactc tacgcgggaa gcaaaaaaca caaggccgca cccgctcggc
     4501 cggatgacgc cgacaccggc gctcagcatg gctccggata gtcgcgttac ggcagggatt
     4561 ctttttcttt tttcttggct tctttttttc gggctttggc catcgccttc agcgcttttt
     4621 tatccggcgg aaacacgggc gaagcggcag cggggggcgt ttcggccacg gggacttgag
     4681 cctgcaaatt cttcatttcc tgcacaggaa cgggaaggcc gttttcccca accgcgccgg
     4741 tgcttagggc tttcggctct tccggatttt gcggtccgtg cgtcagcgga tttttcccgg
     4801 cccggcgaag gcggatcttt tccgtgatgg cttccgccgt catccggccg aactggccga
     4861 taaatcccgc aatcagaacc agaaccaccc acttcagcgc cgaccacgtg ctgatgtaat
     4921 ccagaatgtc cgtcataaag gaagcttcct ttccggcggc cggcctttca caaaaaaacc
     4981 ggcggttcca gcctgcaatg ctcttataca ggaaaacacc cggccatgac aagaactgtt
     5041 ctgctccggc caaaggttga attcattccg ccatcatgat acagattcgg cacattgatc
     5101 catgaacacc gtcggttcgg ggagtttttt catgcgcaag gccatcgtga agtcgattac
     5161 gctgctgttg atcgcctgcg tctcggcttg cgcgccgccg cctctgcaaa agaaagatga
     5221 taagattgcc gcggccctcg cggatccgca gacctctttt tttgaagcct gccgaagagg
     5281 agacatcgcc atggtcatgc gcctggcgga ggaaaaacgg atcgacgtca accaagccga
     5341 cgattacgcc tggacaggcc tgatgtacgc cgttcaaaac ggacacaccg gccttgccga
     5401 atattttctc cttcagaaat cgcaaatcaa ccggcaggat ggggacggac tgacgccgct
     5461 gatgatcgcg gcaaatgagc ggcactcgga catggtggat ctgctgctgg cgttcggcgc
     5521 ggacccgctt ttgcaggatc gacacggcta tacggcgttg atgtgggcca tttacaacaa
     5581 gagctgggac tgtatccccc ccttgctggc agagggaaag gggctcgatg tgcaaaacgg
     5641 cttcggggaa acggcgctgc acctggcgct cgactaccat tgctccgaca ttgccggaat
     5701 cctgatccgg caaaacgcca atgtgaagtt tgcggacggc agaggcagaa ctcctttgca
     5761 tctggcggcg gaaagaggcg atgaagacaa tgcctggctc ttgctctcca gaggagcgga
     5821 cgcaaatgcc ggggatgaat ccggcgaaaa tcctttgatg aaaagcattc aaagcggcaa
     5881 tcctgatctc gccttgctgc ttctccctcg gaccggaaac cttcaggcaa ccaatcggga
     5941 gggtcgtacg gcgctgcacc tggccgtcgt ccgggatttg ccggacacgg cggccgccct
     6001 catccgccgc aaagccgatc tgaacagctc cgacgagcag ggcttcaccc ctctgatgct
     6061 tgccgcggag tcgggttcgg aaagaatgac ggttctgctg gcggagcggg gggccgacct
     6121 gaagatgaag aacaaagagg gcaaaaccgc gcttgatctg gcccgggaaa aaagtcacgg
     6181 caacatcgca acctatctgg aaagaaaaat ggggaagcca tgaaggaaca accggacctt
     6241 ttccgatgga tgcttgccgc cgcgctcacg attgtcctta tcgccggcct gataagttgc
     6301 cggtatctca gcattccggc ccaggatgac gcagtgcccg ctctccttcc atcgaaggaa
     6361 gaaaaaggac cgaccgttta taattctgcg gtgatcaagg gtcgcgtcac cggacccggg
     6421 tggaagggac actccacgct cattctggcc tgccgctccg cggacaaaaa aaacggatgc
     6481 ggcgaatacg cggccacccg tgaaaatcag gatgctttca tgctgtatct gcccgagggg
     6541 cgctattgtc ttattgccgt gacagaccac aatcacgacg gaaagtatga gaaggacgaa
     6601 gcttccggcg cgtatggatt acctcgtcaa atgatggaaa tcaccgtccg ggaaggcgac
     6661 ctgatcaccg gcgtggagat ccggaccgcc gcggccggtg aacgcccggt gcggctgccc
     6721 cgggagtgcc gctggaaaat cgaggagccc gtcattcggc aggcaaccgg caacggggat
     6781 cttctgaaaa tctaccatga atatttttcc ctggaaaacg cgcagaccgg ctactggaat
     6841 ccgtcctcct tcatgaaagc cttcggcgcg catatctacc tgacgcaggc ctacgattca
     6901 cggaaaatcc cgctcctctt cgtgcacggc acggagggaa ccccgcagaa ctggatctat
     6961 tgttacatgc gcctggatcg ggaccggtat cagccctggt tcttttatta tccttcggga
     7021 atccgcctga atctggccgc ggcgctgctt caggaagaac tgcgggagct tcaggaacaa
     7081 tacggctttc gaaaaatggc cctcgccgcg cacagcgtag gcggactgac cgcaaggtct
     7141 tttctgattc gccgcgaatc ggaagaaagg cctcccttca tccggctgtt tgcatccttc
     7201 gccacgccct ggaacggatt cggcctggcc gacgcctccc gggtgctgcc gcacaaaagc
     7261 attccggtgt ggctggatct gggaacgcac agcgaattta tttccgccac catgaacgcc
     7321 aaattgcctc cctcggtccg ccactatctt ttttacggca gggaagacaa actcacggga
     7381 ggcgcggagc cggacagccg ggcaacagcc ggcacggccg gcgtgtatgg attcgactgc
     7441 acgcacgacg gcatcctgat cgaccgcagg gtattttccc gattcaacac catcctggaa
     7501 aaggagatga actgaaatga aaatcatccg gtttctttcc gaaagcggaa agacgcttta
     7561 cggcgcgtac agcaagcgcc ggccggggac ggccgaagga atcgaaaaag aaccctggag
     7621 caagaacatc acaggcggcg gtgaagaaaa aatccggaaa ctgctggcgc ccgtcgcgcc
     7681 cgtcaatatt ctcgcgctgg gcattaatta ccgaaagcac ggcgaggaaa ccgccctgtc
     7741 catcccttcg gagccgatcc tgtttctcaa agccaccagc agcgtcaccg gacccgatca
     7801 tcccattgtg ttgcccgccg ccgggtccga tcaggtggac tatgaagcgg agctggccct
     7861 ggtcatcgga aagacggcca aaaatgtcac gcctgcggaa gcgtgggatt gcattttcgg
     7921 cttcacctgc gccaatgatg tgagcgcgcg cgactggcag ttcgaaaggc agaaaggcca
     7981 gtgggcccgg ggaaaaagct ttgacacctt ctgtccgctg gggccgtgga tcgtcacaaa
     8041 ggatgaaatc gacgaccccg atgatctggc gatccgcgcc gtgctgaacg gaaggacagt
     8101 tcaggaatcc cgcacgtcgg aaatgatctt taatgtgcgg acgatcgtca gccgcctgag
     8161 ccgttccatg acgctgcacc ccggcaccgt gatcctgacc ggcacgccgg aaggcgtggg
     8221 atttgcacgc cggccaccgg tctttcttaa gccgggtgat gtaatccgcg ttgaaattga
     8281 aaaaatcgga acattgagca accgggtgat cgcagaatca tgaaaatcat tcgcacacgc
     8341 tgggttccgc ggcgcaagtt cgcggcgatg aatcttttcg gcattgtttt tgcgccgaag
     8401 ggacaaatcc tcccgccgcg aaccatcaac cacgaagcca tccacactgc gcaaatgagg
     8461 gaaatgctct acatcttttt ttatctgtgg tactttctgg agtggctttt ccggcttaga
     8521 agaaaaacca gcgcctatcg ccggatcggc ttcgagcggg aagcctatca tcatgagcac
     8581 gacctggagt atttaaaaag ccggaagccc taccagtgga tccggtatct ctaaccagcg
     8641 gccggaaacc ggcccgggag aaaaacatgc agacttcatg gaaaaacggc aaagaaaaaa
     8701 tttacacgcg cgccatcgac atctcgactt gggaatatga cgcggaaagg attgtcgtcg
     8761 agggctttct tcgggacgac cgctaccagg acacgcacac cgtcacaggc gaaacgtttc
     8821 cccgcggcac gcttcatcac atgagcattc ggctgctggt caactgctcc accctggtga
     8881 tcgaagatct ggacatggag ctgctttccg taccgcggga ggtctgccgc gaaacgatgg
     8941 gatttctggc cccggtcaaa ggcatgacca tcgcccgggg attcaccgcc cgcatcaaaa
     9001 agctggtggg cgggcccaaa ggctgcgcgc acgtggtgga gcttctgctc gccatggccc
     9061 cggcggtcct gcagggcgtc gccgccgccc ggtcgcgacg tccttccggc gtggatgacg
     9121 ggcagtccaa aatcatcctt caatacctga tcaacacctg ccatgcctgg cgggaagacg
     9181 gcccgttcgt tgaagcgtac gcccaaaaaa tcaaagccat gacgaagagt gccctgtaaa
     9241 gctgcctccg attctgcccc ataaaaaaag ccccctttga aaaatcaaag ggggctttcc
     9301 tgttttaaat ccggcggcac ctactctccc acacagtcgc ccgtgcagta ccatcggcgc
     9361 gaaggagctt aacttccgtg ttcgggatgg gaacgggt
//
LOCUS       DAOPAF010000062         9392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_6931_length_9392_cov_37.897505, whole genome shotgun sequence.
ACCESSION   DAOPAF010000062 DAOPAF010000000
VERSION     DAOPAF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6931_length_9392_cov_37.897505"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLQ77_06435"
     CDS             complement(<1..421)
                     /locus_tag="PLQ77_06435"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPL10348.1"
                     /translation="MMTIIALVIFYLLFPVFAVYMARKNAVCSKLGAVVICYICGIVI
                     GNVGVLPANLGSIQNPLMLITISLALPLMFFSIDVRRWSRLAGKSLLSFGLQTLAILM
                     VAVLGFFIFHGRVGEETWKLAGMFVGLYTGGTVNLGAI"
     gene            complement(510..1271)
                     /locus_tag="PLQ77_06440"
     CDS             complement(510..1271)
                     /locus_tag="PLQ77_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPL10349.1"
                     /translation="MKGGRPMKVVYSEEYKQVYSYDPASNPGRIESIEFALAGKVDYV
                     EPRPATEEEIRAVHTPNHIAQVRREGVYDIAALAAGGAIRAAEIGRSEPCFAVIRPPG
                     HHASSDSAWGFCYFNNMAIALTSLKRQNKLQKAFILDFDLHVGDGNINILGKTGYVTI
                     LNPGGLDRNEYLKTVANALEKTDADMIAISAGFDNHEADWGGLLATEDYTTMGRLVRE
                     AALRNQGGCFGILEGGYNHAVLGKNVLAFIEGLSG"
     gene            complement(1268..2041)
                     /locus_tag="PLQ77_06445"
     CDS             complement(1268..2041)
                     /locus_tag="PLQ77_06445"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPL10350.1"
                     /translation="MCLILFAYHVHPAYRLILAANRDEFYDRPSAPADFWNSHPQMLA
                     GMDLKEKGTWLGVTKEGKFAAITNYRDPASWKADAPSRGKLVSRYLTGSAGPEKYLRN
                     VVKKADIYNGFNLLLGDGADLFVYSNRGNIQKLSPGIYGLSNELLDAPWPKVRRGKKL
                     LKKALAQKGTELEEALFELLSDRAAPPDRNLPDTGIGLEWERLLSTMFIKSPVYGTRS
                     STLLFIGKNRRVKLIEKIYNGEEEPWLVSRFAFPVEGRT"
     gene            complement(2123..2887)
                     /locus_tag="PLQ77_06450"
     CDS             complement(2123..2887)
                     /locus_tag="PLQ77_06450"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPL10351.1"
                     /translation="MDQKIDQVAKLAAKAGKIAVFTGAGISTESGIPDFRGPGGLWTK
                     YDPDDFTIDKFLGSHATRKKMWQRLREGGLMEDAQPNAAHYAVVELEKMGKLLSLVTQ
                     NVDNLHQKAGSSPALIRELHGNMQRLVCLNCRESYPIALVKEKYAGSDDVPTCDYCRG
                     ILKPDVIFFGEALPQQTLAQAMREAEDCDLMIVIGSSLVVYPAAYVPVHARQAGADLV
                     IINKGETEQDHIADVRIDAPAGEVMTKILNRLKAIG"
     gene            complement(2985..3758)
                     /locus_tag="PLQ77_06455"
     CDS             complement(2985..3758)
                     /locus_tag="PLQ77_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPL10352.1"
                     /translation="MKKMAAGIIGGTHGMGRWLAGLLREQGFAVRVAGRETKTTAADL
                     ADRCDVVVVAVPIAATADVIREVGPKVKKGRLLMDLTSLKAEPVALMLAHSEADVVGC
                     HPLFGPSVNEPAGHPIVLCRGRGDDGHVLMKDVFGKAGYPVLETTPEAHDKMMSIIQA
                     LNHFNTIALGMAVAEAGIPFSELQPFSTPMFKTKMEMVQKVFTETPGLYADILAGNPR
                     TKKIMASYARVVRKIRALMDAGDAAGLKKMMEDTAKTLF"
     gene            complement(3755..4831)
                     /gene="aroC"
                     /locus_tag="PLQ77_06460"
     CDS             complement(3755..4831)
                     /gene="aroC"
                     /locus_tag="PLQ77_06460"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPL10353.1"
                     /translation="MSGNTWGTLFCTTTWGESHGPALGAVIDGCPAGIELAVEEIQRE
                     LDRRRPSASAASTSRAEKDRVEILSGVFEGQTTGTPISLLVANADAQSSHYEDLKDVF
                     RPGHGDYTYWKKYGMRDWRGGGRASGRETVARVAAGAVARKVVRAAGMEVTAYTHSIG
                     GIEISSKALKQDPHWLEKVRENSLYCPDSQASAAMEERLSAVRKEGDTLGGVVEILVR
                     GCPAGLGEPVFDKMDADLAKALMSIGTVKAVEVGDGCEVASKKGSEVNDPMDAEGFVK
                     NSAGGILAGITTGQDIVFRVYCKPIPSIALPQQTVDESGRQRSIQVVGRHDVCVIPRI
                     VPVCEAMVLLALADHWLRRKAGVR"
     gene            complement(4849..5412)
                     /gene="aroL"
                     /locus_tag="PLQ77_06465"
     CDS             complement(4849..5412)
                     /gene="aroL"
                     /locus_tag="PLQ77_06465"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HPL10354.1"
                     /translation="MKIILIGCRASGKSTVGRLLSRKLGIPFVDADRLIEKTAGMSIA
                     EIVALKGWQEFRRRETQILASLQEPTVCVLATGGGAVLTDENRKLLKKTGVVIYLKAD
                     LPDVVERLRRDVKHANSRPPLAAGDLIEETRVVLAERTPLYEATADYTVDTRNKNIVQ
                     VAEEINRQLLKAGIVSEIKTLKKKTIP"
     gene            complement(5409..6665)
                     /gene="aroA"
                     /locus_tag="PLQ77_06470"
     CDS             complement(5409..6665)
                     /gene="aroA"
                     /locus_tag="PLQ77_06470"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPL10355.1"
                     /translation="MNSKTNLPENVSSVEVPGSKSLTQRALIAAALATGESLLRGALI
                     AEDTLRLMEGLKLLGARLEQDVSGIRVIGTGGVIANPDREIFLGNNGTALRFLTALVC
                     LGRGMYVLTGEPRLLERPVGPLVDALKDMGVKINCRNNCPPVTVSANGLKGGQIALTD
                     IESSQYVSALLLCAPCTAKGIELSLTGNIVSAPYIDLTVGVMKDFGAKITQTDRQLYR
                     VGTQPIYSGRIYDVEGDASSASYFFLAAALLRRPVRVYGIRRDSAQGDIGLLDVLEKL
                     GCRIESGEAWVEVSAPGGLAAGDVTLDMGNMPDMVPTVGVLAAYRRGKTVITNAAHLR
                     IKESNRLAAMASELGRMGIAASERPDGLTIEGGSPVAANIETYNDHRIAMSFAVAGLV
                     TPGIRIADKKCVEKSFPGFWRELAKL"
     gene            complement(6655..8214)
                     /locus_tag="PLQ77_06475"
     CDS             complement(6655..8214)
                     /locus_tag="PLQ77_06475"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPL10356.1"
                     /translation="MICIPVMEPTQREALRAAAKSAVLADVIELRMDWIADGDVSELI
                     SAARRASAGVKVIVTCRRREEFLLAQENLPALPAKERTGAAKMKLLEKAVLSGADFID
                     IELAEGARAISRMRSLCHKRPNPARLIVSWHDPFKTPSLNGLKKIFDACVQAGADIVK
                     MVPYARSLSDNAAVLSLMDYAKDQGREIIAMCMGEKGQISRILAPSRGSFLGFAVLPG
                     GRPSAPGQLTLREMRAFQKLLSGRQRFFEPPQGDLHFVLLGNPVRQSLSPLMHQAAFR
                     TMGINASYSAFCVTDLAAAVAGIRAMNIQGASVTIPFKTAVMDFLDEIDADAAAVGAV
                     NTIVNDHGRLIGHNTDWSALMDALREETQLKGKKFIILGAGGTARAAAYGITKEGGRP
                     IIVNRTPEKGRKLADRFDCPFYPLMEIGKVRAFGLINTTPVGMFPDSHSSPVDSSILN
                     GFRIVADVIYNPLKTGLIRDAEAKGCRVVPGLEMFVRQGARQLNLWTGRKAPLAVMRN
                     AVRERLMRHEF"
     gene            complement(8211..9290)
                     /gene="pheA"
                     /locus_tag="PLQ77_06480"
     CDS             complement(8211..9290)
                     /gene="pheA"
                     /locus_tag="PLQ77_06480"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPL10357.1"
                     /translation="MKKPLESLREEMRKKDRDIVRLLNERSEISMKIGRVKGQAGFSV
                     YDPAQEAKVIRYLRELNGGPLSDSQVTSIFREILSASRDLQRPTRVAYLGPEASFSHQ
                     ASLLHFGSSCQFFPQQDIARVFDEVEKGEVEWGVVPVENSLEGSVNVTLDRLVLTPLA
                     LQAEIDLRISQCLISQAKSLKGIQKVYSHPQALAQCRSWLRAHLPDAATGEMESTTAA
                     VQMVRGKKTAAAIGSEPAASAYGLNLLARSIEDHSSNTTRFLVIGRGETAPAGDDKTS
                     IIFAAPDVPGALHRALACFAKRKINLAKIESHPVKERLWEYFFFVDMIGHLQDRSVKN
                     CLKELKNKTVYLKTLGSYPRAKSEK"
BASE COUNT         1957 a         2934 c         2447 g         2054 t
ORIGIN      
        1 cgatggcgcc caggttcacc gtgccgcccg tataaagccc cacaaacatg ccggccagtt
       61 tccatgtttc ttcacccacc cggccgtgga aaataaaaaa acccaaaacg gccaccatca
      121 gtatcgccag ggtctgcaag ccgaaggaaa ggagcgactt gccggcaagt ctgctccagc
      181 ggcgtacgtc aatggagaaa aacatcagcg gcagagccag gctgattgta atcagcatca
      241 ggggattttg aatggaacca agattcgcgg gaagcactcc gacattgccg atgacgattc
      301 cgcaaatgta acagatcacg acggcgccga gtttgctgca gacagcattt ttcctggcca
      361 tataaacggc aaacacagga aataaaagat aaaagattac cagcgcgata atggtcatca
      421 tgtcccgata agtttcatga atactgtaga atttgagtcg aagtataaga gattgcagct
      481 tttgtgtcaa aggaataatg acaacaaagt cagccgctca gaccttcaat gaatgccagg
      541 acgtttttcc caaggacggc atgattgtag ccgccttcga gaatgccgaa gcagccgccc
      601 tgattgcgaa gggccgcttc acgcaccagc cttcccatgg tcgtgtagtc ctccgtcgcc
      661 aaaagccctc cccagtcggc ttcgtgattg tcaaacccgg ctgaaatggc gatcatgtcc
      721 gcgtcggttt tttccagagc gttcgcgacg gttttcaggt actcattgcg gtccagtccg
      781 ccgggattga ggatggtcac ataccctgtc ttgccgagaa tattgatgtt gccgtcgcct
      841 acatgcaggt cgaaatccag aataaaggct ttttgcagtt tattttgccg tttgagcgag
      901 gtcagggcga tggccatgtt gttgaagtag cagaaccccc aggcgctgtc ggacgaggca
      961 tgatgccccg gaggacggat cacggcaaag caaggctcgg accgtccgat ttccgcggcc
     1021 ctgatcgcgc cgcccgcggc aagcgcggcg atgtcgtaaa cgccctcgcg ccggacctgt
     1081 gcgatgtgat tcggcgtgtg cacggcgcgg atctcttctt ccgtggcggg ccgcggctcc
     1141 acgtagtcca ctttccccgc cagcgcgaat tcgatggatt cgatccttcc ggggttggat
     1201 gcggggtcat acgagtaaac ctgtttgtat tcctccgaat aaaccacctt catggggcga
     1261 cctcctttca tgtccggccc tccacgggaa acgcaaaccg gctgaccagc cagggctctt
     1321 cctcgccgtt gtaaattttt tctatcagtt taacccgcct gtttttcccg atgaagagca
     1381 gcgttgaaga gcgcgtgccg taaacggggc ttttgataaa catcgtcgag agcagcctct
     1441 cccactcgag gccgatgccc gtgtccggca gattccggtc gggtggcgcg gcccggtccg
     1501 aaagaagctc aaacagcgcc tcttccagtt ctgttccttt ctgggccagg gctttcttca
     1561 ataatttttt gccgcgccga accttgggcc agggcgcatc gaggagctcg ttgctcaagc
     1621 cgtaaatccc gggagaaagt ttttggatgt tcccccggtt ggaatacaca aagaggtccg
     1681 cgccgtcgcc caaaagaagg ttaaacccat tgtaaatgtc ggcttttttg acaacattcc
     1741 gtagatattt ttcggggccg gcagatccgg tcagataccg gctgaccagc ttgccgcggg
     1801 acggcgcgtc cgctttccag gaagcgggat cgcgataatt ggtaatggcg gcgaatttgc
     1861 cttctttcgt gacgcccagc catgttcctt tttcttttaa atccatcccc gccagcatct
     1921 gcggatgact gttccagaaa tccgccggcg cggaaggccg gtcgtagaat tcatcgcggt
     1981 tggccgccag gatcaggcgg tatgcgggat gcacatgata ggcaaaaagg atcaggcaca
     2041 taagaatcct caggcggtta gactgaaggc tgaagactga aggtcaaaag gaacgggcgc
     2101 acagcgcccc tcaaaacagc ctttagccta tcgcctttag cctattcaat attttcgtca
     2161 tgacctcccc ggccggggcg tcaatgcgaa cgtcggcgat gtgatcctgt tcggtttcgc
     2221 ccttattgat gatcaccaga tcagcccccg cctgcctggc atgaaccgga acataggcgg
     2281 cggggtaaac caccagcgac gagccgatca cgatcatcaa atcgcagtct tccgcttccc
     2341 gcatcgcctg agccagcgtc tgctgaggca gggcctcccc gaagaaaatc acatcgggct
     2401 tgaggatgcc ccggcagtaa tcgcaggtcg gcacatcgtc ggaaccggca tatttttcct
     2461 tgaccagggc aatcgggtag ctctcacggc agttcaggca aaccagcctc tgcatgttgc
     2521 cgtgcaattc ccggatcagc gcgggcgaac tccccgcctt ctgatgcaga ttgtccacat
     2581 tctgcgtgac taaagacagc agtttgccca tcttctccag ctccaccacg gcataatgag
     2641 ccgcgttggg ctgcgcgtct tccatcaggc cgccttcccg caggcgctgc cacatttttt
     2701 tgcgagttgc gtgagatccc agaaatttat cgatggtgaa atcatccgga tcgtacttgg
     2761 tccagagccc tccggggccg cgaaaatcag gaatgccgga ttccgtgctg atgcccgcgc
     2821 cggtgaaaac ggcaattttt ccggctttcg ccgccagctt cgcaacttga tcgatttttt
     2881 gatccataaa agacgggcgc tcctttgatt aaaacgttat gcagtgtgaa caaaatacac
     2941 catctgtctc aaaaatcaaa agaaaatctc ccgtccggac cgtctcagaa cagcgttttt
     3001 gccgtatcct ccatcatctt cttgaggccg gcggcgtcgc ctgcgtccat cagggcgcga
     3061 atcttccgca cgacgcgtgc gtaggacgcc atgatctttt tcgtgcgggg attcccggca
     3121 agaatgtctg cgtaaagccc cggcgtttcg gtaaacacct tttgcaccat ctccattttg
     3181 gttttaaaca tcggcgtgga aaacggctgc agctccgaaa aaggaattcc agcttcggca
     3241 accgccatgc ccaacgcgat ggtattgaaa tggttgaggg cctgaatgat cgacatcatc
     3301 ttgtcatgcg cctcgggcgt tgtctccaga accggataac cggcttttcc aaaaacgtcc
     3361 ttcatcaaaa catggccgtc atcgccccgc ccccggcaca gaacgatggg atgtccggca
     3421 ggctcgttga ccgacgggcc gaacagcgga tggcagccga cgacatccgc ctcggaatga
     3481 gcgagcatca gcgccaccgg ctccgctttc agcgacgtca aatccatcag cagccggcct
     3541 ttcttcactt tcggtccgac ttcccgaatg acgtccgccg tcgccgcaat cggaacggca
     3601 accaccacca cgtcgcaccg gtcggccaga tccgccgccg tggttttggt ttctctgccc
     3661 gccacgcgaa cggcaaagcc ctgctcacga agcagaccgg ccagccagcg gcccatgccg
     3721 tgcgtgccgc cgataatgcc cgccgccatt ttcttcatcg gacacccgct ttccgccgaa
     3781 gccagtggtc cgccagcgcc agcagaacca tcgcttcgca aaccggaacg atcctcggaa
     3841 tgacgcaaac atcgtgtctg ccgacgacct gaatcgaacg ctgccggccg ctttcgtcca
     3901 ccgtctgctg cggcagtgcg atggagggga tgggtttgca gtacacccgg aagacaatgt
     3961 cctggccggt ggtgatgccc gcgaggattc cgcccgcgga atttttgaca aagccttcgg
     4021 catccatcgg atcgttgacc tcggaccctt tttttgacgc gacttcacag ccgtctccca
     4081 cttccacggc cttcaccgtt ccgatgctca tcagggcttt ggccagatcc gcgtccatct
     4141 tgtcaaaaac cggctccccc aaaccggccg ggcatccccg gacaagaatc tccacaacgc
     4201 ctcccagcgt atccccttcc ttccgaacgg cggacagtcg ctcctccatt gccgccgacg
     4261 cctgcgaatc cggacaatac agggagttct cccgaacttt ttccagccag tgcggatcct
     4321 gcttcagagc cttcgaagaa atctcaatgc cgccgatgct gtgagtatag gcggtcactt
     4381 ccatgccggc ggcccggacc actttccggg ccacggcccc cgccgcaacg cgggcgacgg
     4441 tttcgcgccc cgaagcacga cctcctcccc gccagtcccg catgccgtat tttttccagt
     4501 acgtgtagtc cccgtggccg ggacgaaata cgtctttcaa atcctcgtag tgcgaagatt
     4561 gcgcgtcggc atttgccacc agaagagaaa tcggcgtgcc ggtggtttga ccctcgaaaa
     4621 cgccggacaa aatttccacc cggtcttttt cggcgcggga cgtggaagcc gctgacgcgg
     4681 aaggcctgcg gcgatccagc tcccgctgaa tctcttcgac agccagttcg atcccggccg
     4741 gacagccgtc aatgaccgcg cccagcgctg ggccgtgcga ctccccccac gtcgttgtgc
     4801 aaaacaaggt tccccacgta ttgcctgaca tacatcctcc aacaatcgtt atgggatcgt
     4861 cttctttttc aacgttttaa tttcggagac gatgcccgcc ttcaaaagct gccggttaat
     4921 ctcttccgcc acctgtacga tatttttgtt tcgagtgtcc accgtgtaat cggcggttgc
     4981 ctcgtaaagg ggagtgcgct ctgccaaaac gacgcgggtc tcttcgatca gatctcccgc
     5041 ggcgagcggc ggacggctgt tcgcgtgttt cacatcgcgc cgcagccgct ccacaacatc
     5101 cggcaggtcc gctttcaggt aaatcacgac gcccgttttt ttcagcagct ttctgttttc
     5161 atctgtcaaa accgctcctc cgccggtcgc caggacgcaa accgtcggct cctgaagaga
     5221 agccagtatt tgcgtttctc tccgccggaa ttcctgccag cctttcagcg cgactatttc
     5281 cgcgatggac atccccgccg ttttttctat aagccggtct gcgtccacaa agggaatgcc
     5341 gagttttcgc gacaaaagcc tgcccaccgt ggattttccg gacgcgcggc agccaatcag
     5401 gatgattttc ataatttggc cagctcccgc cagaaacccg gaaaagattt ttccacgcat
     5461 tttttgtcgg cgattctgat ccccggcgta accagccccg caacggcaaa gctcatcgcg
     5521 atgcggtggt cgttgtatgt ttcaatgttc gccgcgacag gcgagccgcc ctcgatcgtc
     5581 aaaccatccg gccgttcgga cgcggcgatc cccatccggc caagctcgct tgccatcgca
     5641 gccaggcggt tgctttcctt gattctcaga tgggcggcat tggtaatcac cgttttcccc
     5701 ctgcgatacg ccgccagaac tccgaccgtc ggcaccatgt ccggcatatt tcccatgtcc
     5761 agcgtcacat cgcccgcggc aaggccgccg ggcgcgctga cttccaccca cgcctcaccg
     5821 ctttcgattc ggcagcccag tttctccaga acgtccagca ggccgatgtc cccctgggcg
     5881 gagtcccggc gaataccgta aacgcggacc ggccttctca aaagggctgc cgccaggaaa
     5941 aaataggagg cgctggaggc atctccttcc acatcgtaaa tccgtcccga ataaatcggc
     6001 tgcgttccga ccctgtatag ttggcggtct gtttgcgtaa ttttcgcgcc gaaatccttc
     6061 atgacgccga cggttaaatc aatgtagggc gcggaaacga tatttcccgt gagggaaagt
     6121 tcgatcccct tggctgtgca gggagcgcaa agcaaaagcg ctgaaacata ttgggagctc
     6181 tcgatgtccg tcagtgcaat ttgcccgccc ttcagtccgt tggccgatac cgtcaccggc
     6241 ggacaattat ttcgacagtt gattttcacg cccatgtcct tcagagcatc caccagaggg
     6301 ccaaccggcc tttccagcag acgcggctcc ccggtcagaa catacatacc gcgtcccaga
     6361 caaaccagcg ccgttaaaaa ccgcagcgcc gtgccgttgt ttcccaaaaa tatttcacgg
     6421 tccggattgg caatcactcc gccggttccg ataaccctta tgccgcttac atcctgttcc
     6481 agcctcgcac ccagcagctt cagaccttcc atcaggcgca gggtgtcctc cgcaatgaga
     6541 gcgccccgga gcaacgactc tccagtggcc agcgcggcgg caatcaaagc ccgctgcgtc
     6601 agactctttg atccgggcac ctccacgctt gaaacatttt ccggcaggtt tgttttagaa
     6661 ttcatgcctc atcaaccttt ccctgactgc gtttctcatc acggccaggg gagcttttct
     6721 gcccgtccat aagttcaact gccgcgctcc ctggcggaca aacatctcca gcccggggac
     6781 aaccctgcac cctttcgcct ccgcatcgcg aatcaatccg gtcttcaggg ggttataaat
     6841 gacatccgcc acgatacgga agccgttcag gatggacgaa tcgaccggcg atgaatgaga
     6901 atccggaaac atgcccaccg gcgttgtatt gatgaggccg aaagcccgga ctttgccgat
     6961 ttccatcaag ggataaaacg gacaatcgaa ccggtcggcc agtttcctgc ccttctccgg
     7021 cgtccgattc acaatgatcg gccgtccgcc ttcttttgta attccatacg cggcggcccg
     7081 cgctgttccg cccgccccca gaatgataaa ttttttgccc ttcagctgcg tttcctcacg
     7141 caacgcatcc atcagggcag accagtcggt attatggcca atcaggcggc cgtgatcatt
     7201 cacgatcgtg ttgaccgctc ccacggctgc cgcatccgcg tcgatttcgt ccagaaagtc
     7261 cataaccgcc gttttgaacg gaatcgtaac cgatgccccc tgtatgttca ttgcccgaat
     7321 gccggctaca gcggcggcca gatcggtgac gcaaaaggca ctgtaagaag cgttgatgcc
     7381 cattgtccgg aaagccgcct gatgcatcaa cggcgacaaa ctctggcgaa cggggtttcc
     7441 cagaagaaca aaatggagat ccccctgcgg cggctcaaaa aatcgttgac ggccggacaa
     7501 aagtttctga aaagccctca tctccctgag cgtcaactgg ccgggcgcgg agggccgtcc
     7561 gcccggcaag acggcgaagc ccaggaagct tccccgcgac ggcgccagaa tccggctgat
     7621 ctgacccttc tcgcccatgc acatggcgat gatttcccgc ccctgatctt tcgcgtaatc
     7681 catcaggctc agtacggcgg cgttatcaga caggcttcgg gcatacggga ccatcttcac
     7741 gatatctgcg ccggcctgca cacaggcatc aaatattttc ttcaacccgt ttagcgacgg
     7801 ggttttgaac gggtcgtgcc aggaaacgat cagccgcgcg ggatttggcc gcttgtggca
     7861 cagggatcgc attctggaga tggctcttgc cccttccgca agctcgatat caatgaaatc
     7921 cgcgccggat aaaacagcct tttcaagcag tttcattttg gccgctccgg ttctttcttt
     7981 cgcgggaagg gccgggaggt tttcctgtgc aagcaaaaac tcttctctcc tgcggcacgt
     8041 cacgatgact ttgacgcctg cggacgcgcg ccttgctgcg ctgatcaact ccgacacgtc
     8101 tccgtccgcg atccaatcca tgcgcaattc gatcacgtcg gccaggacgg cgctttttgc
     8161 cgccgcccgg agagcttccc gctgcgtcgg ttccatgacg ggtatgcaga tcatttttca
     8221 ctcttggccc gcggatagga ccccagtgtt ttcagatata cggttttgtt tttcaattcc
     8281 ttgaggcagt ttttcaccga ccggtcctgc agatgaccga tcatatccac aaaaaagaaa
     8341 tattcccaca gcctttcctt cactggatgc gactcgatct tggccagatt gatctttcgc
     8401 ttcgcgaaac acgccagcgc ccggtgcagc gccccgggaa catccggcgc ggcgaaaata
     8461 atcgaggttt tgtcatcgcc ggcaggcgct gtctcgcccc ggccgatcac cagaaaacgc
     8521 gtcgtgttgg aggaatggtc ctcgatgctt cgcgcgagca ggttcaaacc gtaggcggaa
     8581 gccgcaggct cacttccgat ggctgccgcc gtttttttcc cccgcaccat ttgaacggcg
     8641 gccgttgtgc tttccatttc acccgttgcg gcgtcgggca gatgcgcgcg aagccaagac
     8701 cggcactgcg ccagcgcctg cggatgcgaa tagacctttt ggatgccttt caggcttttc
     8761 gcttgtgaaa tcaggcactg gctgatgcgc agatcaatct ccgcctgaag ggcaagcggc
     8821 gtcagcacca gccggtccag cgtgacattg actgaacctt ccagtgaatt ttctacggga
     8881 acgacgcccc attccacctc tcccttttcc acttcatcga aaacccgcgc aatgtcctgc
     8941 tgcggaaaga attggcagga ggagccgaaa tgcaaaagcg aagcctgatg gctgaaagac
     9001 gcctccggcc ccaggtaggc gacgcgcgtt ggcctctgca aatcgcggga ggccgagagg
     9061 atctcccgga aaatggacgt gacctgactg tccgacagcg ggccgccgtt cagttcacgc
     9121 aggtaccgga tcactttggc ttcctgcgcc ggatcgtaaa ccgaaaaacc ggcctgcccc
     9181 ttcacccggc caatcttcat ggatatttcc gagcgttcat tgagcagccg gacaatatcc
     9241 cgatcctttt ttctcatctc ttcccttagg ctctccagcg gtttcttcat gctttcatgt
     9301 cctccagaac gccgcggatc gtttcgtcct cgatgccgcc gttgacaaac ggcatgccga
     9361 ttttccggat caggacaaaa tggaccacat ta
//
LOCUS       DAOPAF010000063         9029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_7255_length_9029_cov_51.899933, whole genome shotgun sequence.
ACCESSION   DAOPAF010000063 DAOPAF010000000
VERSION     DAOPAF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_9029_cov_51.899933"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLQ77_06485"
     CDS             <1..275
                     /locus_tag="PLQ77_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPL10358.1"
                     /translation="SAPDIAGKNIANPLATILSVALMLRHSLDRPEEAALVEQSVKSV
                     LREGLRTPDIAAAGGGAATTEEMGEAVVRNLSSLLRGTAGKEVKSP"
     gene            272..676
                     /locus_tag="PLQ77_06490"
     CDS             272..676
                     /locus_tag="PLQ77_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL10359.1"
                     /translation="MKGKILIVDDEESMRRLLSQLFSAEGYEVAAAENGPSALEILRH
                     SVIDVIFLDLKLFSMDGIELCRKIRETQPVSMIFAVTGWSPLFGIEECRGAGFDDYFE
                     KPLDMKLILASVEDAFGKLNRWKRLYSNPSTP"
     gene            689..1309
                     /locus_tag="PLQ77_06495"
     CDS             689..1309
                     /locus_tag="PLQ77_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10360.1"
                     /translation="MPNSAILLKNIVIAPPAAQIYSRLGFKRATTSLSSGRRRETDRH
                     MEEAASMIRLQGSFLRLSIRRNDGRLISLQGRTTFRSEKLSAFLGDCRDAVLMGATAG
                     KPIMDAIAEETARGNLTAAVVYDATASEMTDAALDWIMNYVNGQLRREGRRLLPRRFS
                     AGYADFSLSSQKVIHKKLRMEEIGVSITPACILQPEKSVTAIGAIP"
     gene            complement(1353..2570)
                     /locus_tag="PLQ77_06500"
     CDS             complement(1353..2570)
                     /locus_tag="PLQ77_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10361.1"
                     /translation="MLNHDLSFFYYIVLFAAYCFIGWVAEVMYRSAAQRKFVNAGLLF
                     GPFIPIYGIGAFLVVVLEYALQGRPIALRLAAFGLVLTLLEYAAGYLSEKIFKLKLWD
                     YSEYRFHLHGRVSLLFSFFWTVLAFGFVTMIHPEALRLSALLPAGPVQIAAIVFMVYF
                     WIDYTFSVMSLAAFRKGVAYLYEEYFNLSSLEIEDILKSFQRLREAFPDLNRYVNQNI
                     NRRIRIKIGSFLKPVLEKFFQELDGRKPFEAEYYETVREILDHEEFRKLKDYFHHNSS
                     IYRHVHDVAYLSYRISKFLKLDFRSTARGALLHDFFLYDWRHHDAPDLPREKFHGLEH
                     PKIAAANAKKYFSLNDIEEDIIRKHMWPLTIVPPKYKESYVVSFADKYLSSREFISEY
                     KKRIEKRRSKASE"
     gene            2714..3661
                     /locus_tag="PLQ77_06505"
     CDS             2714..3661
                     /locus_tag="PLQ77_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPL10362.1"
                     /translation="MEKIRLGISTCLLGENVRYDGGHKLDRFLRDTLGRYVDYVPVCP
                     ETECGMGIPREAMRLEGRPDAPRLVTRTSRIDKTDMMVSWANMRIAKLASEELCGFIF
                     KSDSPSSGMERVKVYDEKGMPAKTGVGIFARMFMDAFPLLPVEEEGRLHDPQLRENFI
                     ERIFALRRWREVRRARSPRGALVDFHTRHKLLLLSHSTKLYQAMGKLVAEQKRHALQD
                     LWAKYEALLMEALKLKTTPKKNVNVLMHMMGYFREKLSADEKQELLEIFERCAQGHVP
                     LIVPLTLIGHHVRKYDEPYLKEQVYLNPHPLELQLRNHV"
     gene            complement(3686..4576)
                     /gene="htpX"
                     /locus_tag="PLQ77_06510"
     CDS             complement(3686..4576)
                     /gene="htpX"
                     /locus_tag="PLQ77_06510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPL10363.1"
                     /translation="MKRVLLFIGTNIAVLVVLSIVIKLLGVDQILYANGINYLNLLIF
                     SAVFGFGGSFISLAMSKWMAKWSTGAQVIMQPRSDAEIWLVNTVQKQAAQAGIGMPDV
                     AVFESPSPNAFATGMNKNNALVAVSTGLLRSMNRNEVEAVLGHEISHVANGDMVTLSL
                     IQGVVNTFVIFLSRIVGYFVDRVVLKNEEGHGIGFFITTIVAQIVFGILASVIVMWFS
                     RRREYRADAGGARLAGVGSMIAALESLQRSTFDPLPDQMQAFGISGKPGKNSLKLLFM
                     THPPLEDRIEALKRSAGQAA"
     gene            4920..5336
                     /gene="rnk"
                     /locus_tag="PLQ77_06515"
     CDS             4920..5336
                     /gene="rnk"
                     /locus_tag="PLQ77_06515"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPL10364.1"
                     /translation="MADRTIHITQFDMDRLTELIDGLRLSPKANQANLDLLEKELYRA
                     VLVDPPNIPRDVITMNSKVIITDTESGEKTTYTLVFPSAANISENKLSIMAPLGMALL
                     GYRTGDIIEWPVPSGVRKLKVDKIIYQPEASGDYHL"
     gene            5402..6406
                     /gene="bioB"
                     /locus_tag="PLQ77_06520"
     CDS             5402..6406
                     /gene="bioB"
                     /locus_tag="PLQ77_06520"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPL10365.1"
                     /translation="MDLIDALKHKALKGKGIRESEALELFIEGSSSPWRVFAAASELR
                     ERFKGKEIIFCGITNAKSGRCPEDCKFCAQSSHNNASAPIYPLKPARQIIAEAEQAGK
                     NGAEFFGIVTSGKSVRSKKEWSEIFKAVEHVAAIGIHPCASLGLLDADRARDLKAAGL
                     FRYHHNLETARSYYRNICKTHDYEENVQTIRVARDAGLSVCSGALFGMGENITHRIEL
                     AADLHRLSVDSVPINLLHPIKGTPLSHMPLLPPMEILMTIAVYRFLLPDRDIKLCGGK
                     EKSLRQLLPLGIIAGANSLMTGNYLTTTGRDSRLDHEMIIDLDLVPTRPLDICKCGQD
                     "
     gene            complement(6403..7239)
                     /locus_tag="PLQ77_06525"
     CDS             complement(6403..7239)
                     /locus_tag="PLQ77_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HPL10366.1"
                     /translation="MTGFSEVSLMYDSPYSASSDVSNARAQDNVDLSISRIEVLAFRT
                     TFDVKKRRGMVPVNVSFFPEKQFARALKIMRPVFAKNLAVSSRVAVAKAGESLGDVVV
                     PEGKTGLATICSIVVNGVLLKHGIPVDSKFGGILQLRKGQGLRFVELIHYASSSLDPS
                     EIFIRGKMTSIGQVVANGDGKILANFREIPALSRTLVNCVIDDFTRAGINAVLSIGDI
                     GHPICQTHVDLNKIGMIMIGGLNPVVAVSEAGIPVDNRAMSTVMEFEQLQDIGKGKGS
                     NL"
     gene            complement(7358..8938)
                     /locus_tag="PLQ77_06530"
     CDS             complement(7358..8938)
                     /locus_tag="PLQ77_06530"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPL10367.1"
                     /translation="MSFSRVNTSSATLTKNRTEESISPTSGMCVTCIDGCVGMCEIGK
                     SAYRGHEVIYPQPFGVITCAGEKKYPVDYSHFNIMGTAAGAYGIEADSDKAIFPAVNL
                     EVVFGHDKGIKFKLPWLISGIGSTNIAKNNWEGLAIGSALAGTGLTIGENVVGMDPQS
                     KFKNGNILDTVDLKRRVKLYRDYQRFGYGAIIVQANLEDTRLNVHEYAIEELGVECVE
                     LKWGQGAKDIGGEVKIKDLKKAQMLHDRGYIVLPDPYDSNVIEAFARGAFKEFERHSR
                     VGMVTEESFAATIKRLRKAGAKYIFLKTGAYRPVDLARAIYFSSKYKLDLLTVDGAGG
                     GTGMSPWRMMNEWGVPPVELHTLVYQYAKKLAAKKKYLPAIAVNGGFTFEDQIFKGLA
                     MGAPFVKLIGMARAPIAAAMVGKTIGQTIDAQQVPVYVERFGNTKDEIFVTASELRHE
                     LGHKEFEQLPTGAIGLYTYYERLAQGLRQLMAGSRKFALKYMTRDDLAALTREAADVS
                     GIQYIMDVDKKEAEKILK"
BASE COUNT         2067 a         2602 c         2441 g         1919 t
ORIGIN      
        1 gctccgcgcc ggacatcgcg gggaaaaaca tcgccaaccc gctggcgacg attctgtcgg
       61 tcgccctgat gctgcggcat tcactggacc gccccgaaga ggcggcgctg gtcgaacaat
      121 cggtgaaaag cgttctgcgg gagggcctgc gcacaccgga catcgccgcg gcgggcggcg
      181 gggcggccac aaccgaagag atgggcgaag ccgtcgtccg gaatctttcc tccctcctgc
      241 gggggacggc cggaaaggag gtcaaatcgc catgaaaggc aaaattctga tcgtggacga
      301 cgaggaatcc atgcggagac tgctttccca gctcttttcc gcggaaggct acgaggtcgc
      361 tgccgcggaa aacggtcctt cggcgctgga gattcttcgg cacagcgtca tcgacgtcat
      421 ttttctggat ctgaagctct tcagcatgga cggcatcgag ctctgccgga agatccgcga
      481 aacacagccc gtctccatga tttttgccgt caccggatgg tctccgctgt tcggcatcga
      541 ggaatgccga ggggccggct ttgacgatta ttttgaaaag ccgctggata tgaagctgat
      601 cctggcctcc gtcgaagacg cctttggaaa gctgaaccgg tggaaaagac tgtattcgaa
      661 cccctccact ccataagccg gacgactcat gcccaactcc gccattttgc tgaaaaacat
      721 tgtgatcgcc ccgcccgcgg cgcagatcta ttcccggctt ggatttaaaa gagcgaccac
      781 gtccctttca tccggccgcc ggcgggaaac ggaccggcac atggaagaag cggcctcgat
      841 gattcgactg cagggctcct tcctgcggct ttccatccgc cggaatgacg ggcggctcat
      901 ctcccttcaa ggacggacga ccttccgcag cgaaaagctt tccgcgtttc tgggcgactg
      961 ccgggatgcg gtgctgatgg gggcgactgc cggcaaaccg atcatggacg ccatcgcgga
     1021 agaaaccgcc cggggaaacc tgaccgccgc cgtcgtttac gacgcaaccg ccagtgaaat
     1081 gaccgacgcg gcgctcgact ggatcatgaa ttatgtgaac ggtcagctcc ggcgcgaagg
     1141 caggcgcctg ctgccgcgcc gcttttccgc gggctacgcg gacttttccc tgtccagcca
     1201 aaaagtcatt cacaaaaaac tgcgaatgga agaaatcggc gtctccatca cgccggcgtg
     1261 cattcttcag ccggaaaagt ccgttaccgc aatcggcgcc atcccctgaa caaaaccaag
     1321 ccgcccgcgg ccccatcgca aaaatgcgct gttcattcgg acgccttcga ccggcgcttc
     1381 tcgattcttt ttttgtactc gctgatgaat tccctggaag acagatactt gtccgcaaat
     1441 gaaacgacgt aggactcctt gtatttcggc ggcacgatgg tgagcggcca catgtgcttg
     1501 cggatgatgt cctcttcaat gtcgttgaga gaaaaatatt ttttggcgtt ggccgcggcg
     1561 attttcggat gttccaggcc gtggaatttt tccctgggca aatccggcgc gtcgtgatgg
     1621 cgccagtcat agagaaaaaa gtcgtgcagc agcgcgccac gggccgtgga gcggaaatcg
     1681 agcttgagaa atttgctgat ccggtaggac aggtaggcca cgtcgtgaac atggcgataa
     1741 atggaggagt tgtgatggaa gtagtccttg agcttccgga attcctcatg atcgagaatc
     1801 tcccgcacgg tctcgtaata ctccgcttca aacggcttcc ggccgtcaag ctcctggaag
     1861 aatttttcca aaacgggctt tagaaacgaa ccgatcttga tccggatgcg ccggttgatg
     1921 ttctggttga cgtaccggtt caggtcggga aaagcttccc gcagccgctg aaaggatttc
     1981 aaaatatcct cgatttcgag gctgcttaag ttgaagtact cctcgtagag ataggcgacg
     2041 cccttgcgga aggcggccag cgacatcacg gagaaagtgt agtcgatcca gaaatacacc
     2101 atgaagacga tggcggcgat ctgcacgggg cccgcgggca gcagggccga aagccggaga
     2161 gcttcgggat ggatcatcgt cacaaacccg aaggccagga cggtccagaa aaaggaaaaa
     2221 agaaggctca cgcggccgtg gagatggaag cggtattccg agtagtccca aagcttcagt
     2281 ttgaagatct tttcggacag atagccggcc gcgtattcaa gaagcgttag caccaggccg
     2341 aaggcagcca gacgaagcgc gatgggccgc ccctgcagag cgtactccag gacaacgacg
     2401 agaaaggcgc cgatgccgta aatgggaatg aaggggccga acaataatcc cgcattgacg
     2461 aacttccgct gagccgcgga gcggtacatc acctccgcca cccatccgat aaagcagtac
     2521 gccgcaaaca gaacaatata atagaagaag ctcagatcat ggttcagcat ggttttcccc
     2581 ggccttttta tgcaggccga tgctacacaa ttcccctctt tttataaaga tattttgcat
     2641 ccatctgtgg tatgagaagg agcctttgaa cacaccgcct gccgtccatg gacggaagcg
     2701 ggaaggagac gccatggaaa aaatcagact cggcatcagc acctgtcttc tgggagagaa
     2761 cgtccgctat gacggcggac acaaacttga ccgtttcctg cgagacacgc tggggcggta
     2821 tgtggattat gtgccggttt gcccggagac cgagtgcggc atgggcattc cccgcgaagc
     2881 catgcgcctg gaaggcagac cggacgcgcc ccggctcgtc acgcgcacca gccgcatcga
     2941 caaaacggac atgatggttt cctgggcgaa tatgcgcatc gcaaagctcg cgtccgagga
     3001 gctctgcggc ttcattttca agagcgattc gccgtccagc ggcatggaaa gagtcaaggt
     3061 ctatgatgag aaaggaatgc ccgccaaaac cggcgtgggc atcttcgcgc ggatgttcat
     3121 ggacgctttt cccctgctcc cggtggaaga ggaagggcgg ctgcatgatc cgcagcttcg
     3181 cgaaaacttc attgaaagaa tttttgcgct caggcgctgg cgcgaagtcc gcagggccag
     3241 gtccccccgg ggcgccctgg tggattttca cacacggcac aagctgcttt tgctgtcgca
     3301 cagcacaaaa ctgtatcagg ccatgggaaa actggtcgcc gaacaaaagc gccacgccct
     3361 gcaggatctt tgggcaaagt acgaagcgct gctcatggaa gcgctgaaac tcaaaaccac
     3421 gccgaaaaag aatgtcaatg tgctgatgca catgatgggc tactttcggg aaaaactctc
     3481 cgccgatgaa aaacaggagc ttctggaaat ctttgagcgc tgcgcgcagg gtcatgttcc
     3541 cctgatcgta cctctcacgc tgatcggcca ccatgtgcgc aaatacgatg aaccctatct
     3601 gaaggagcag gtgtatctga atcctcaccc gctggagctt caactgcgca accacgtata
     3661 aaccggccgc ccgcgcgaat ctttgttacg ccgcctgtcc ggcggagcgc ttcagcgctt
     3721 cgatgcggtc ttccagcggg ggatgcgtca taaacagaag ctttagactg tttttgccgg
     3781 gctttccgct gatgccgaag gcctgcatct gatccggcag gggatcaaac gtgctgcgct
     3841 gcagactctc cagcgcggcg atcatgctgc ccacgcccgc aagccttgcg ccgccggcat
     3901 ccgcgcgata ttcccgccgg cggctgaacc acatgacaat gacgctggcc aggatgccga
     3961 agacaatctg cgcgacgatt gtggtgataa aaaatccgat tccgtggcct tcttcgtttt
     4021 tcagaacaac acgatccaca aaatatccga cgatacggga cagaaaaatg acaaaggtgt
     4081 tgacgacgcc ctgaatcaaa ctgagcgtca ccatgtcgcc gttggccacg tggctgatct
     4141 cgtggcccag caccgcctcg acttcattgc ggttcatgct gcgcagaagc cccgtgctga
     4201 ccgccaccag cgcgttgttc ttattcatgc cggtagcaaa ggcgttgggc gacggcgatt
     4261 caaaaaccgc tacatccggc atgccgatgc ccgcctgagc agcctgcttc tgaaccgtgt
     4321 tcaccagcca gatttccgcg tcggagcggg gctgcatgat gacctgggcg ccggtgctcc
     4381 acttggccat ccacttggac atggccagcg atatgaacga tcctccgaag ccgaaaaccg
     4441 ccgaaaaaat cagcaggttt aagtaattga tgccattggc gtaaagaatt tgatccacgc
     4501 cgagaagctt tatgacaatg cttaagacca cgagcacggc gatgttggtc ccgataaaca
     4561 gcaaaactct tttcattttg atctcctccg gtttaaaatc gcgcaaactg tttgcctggc
     4621 aaacctttta aaatgatgcc ttatcttaat gtcaggtcac ggaatgtcaa gcgtttcttc
     4681 tccggcggcc ctcacccccg caaatcctcc gccggaatgc cgctgcgggg atttgagcgg
     4741 aaaagatccg gattgcctgt tctttttttt tgaaaactgt gatagggtgc tttgcaattc
     4801 ggagcatcgg ccaggacggt gttccgtttt ttatcaaaca ataatcgccg tttttgcggc
     4861 ttttggatcc ggcaaccgtt ttcaaccccc agaccaggcc aaaaggagga cacagcatca
     4921 tggcagaccg cacaatacac attacgcaat tcgacatgga caggctgacg gaactgatcg
     4981 acggtttgcg tttatcgccc aaagccaatc aggccaatct cgatctgctg gaaaaagagc
     5041 tgtatcgcgc cgtgctggtg gatcctccga atatcccccg ggatgtcatt accatgaatt
     5101 ccaaagtgat catcacggac acggaatccg gcgaaaaaac aacttatacg ctggtgttcc
     5161 cgtccgcggc caacatatcg gaaaataaac tttccatcat ggccccgctg ggtatggcgc
     5221 ttttgggata ccgcacgggc gacatcatcg aatggcccgt tccgtccggc gtccgcaagc
     5281 tgaaagtgga taaaatcatc tatcagccgg aggcttcagg ggattaccat ttatgaagaa
     5341 cggcccgatg cctgtatcct ccccccggcc ggccatacaa aaaaccaagg gaataacgaa
     5401 catggatctg atcgatgcgc tgaaacataa agccttgaaa ggaaaaggca tccgggaatc
     5461 cgaagcactt gagctcttta ttgaagggag ctcgtcgccg tggcgcgttt tcgccgcggc
     5521 ctcggaactc cgggagcgtt ttaaagggaa agaaattatt ttttgcggca ttacaaacgc
     5581 caagtccggc cgctgcccgg aagactgcaa attctgcgcg cagtcctcac acaacaatgc
     5641 ttccgccccc atttacccgc tcaagccggc cagacaaatc atcgccgaag ccgaacaggc
     5701 cgggaaaaac ggggcggagt tcttcggcat cgtcaccagc ggaaaaagcg tcagatccaa
     5761 aaaggaatgg tcggaaattt tcaaggccgt cgagcacgtg gcggcaatcg gcattcatcc
     5821 ctgcgcgtcc ctgggactgc tcgacgcgga ccgggcccgc gacctcaaag ccgccggact
     5881 gttccgctat catcacaatc tggagacggc ccgcagctat taccgcaaca tctgcaaaac
     5941 gcacgattac gaggaaaatg tgcaaaccat ccgcgttgcc cgggacgcag gcctgtccgt
     6001 ctgttccggc gcgctcttcg gcatgggtga aaacatcacg catcgcatcg aacttgccgc
     6061 cgacctgcac cggctgagcg tcgattcggt gccgatcaat ctgctccatc cgatcaaggg
     6121 cacgccgctt tcgcacatgc cgcttctgcc gccgatggaa attctcatga ccatcgccgt
     6181 ttaccggttc ctgctgccgg acagggacat caaactctgc ggcggaaaag aaaaaagtct
     6241 gcgccagctt ctgccgctgg gcatcatcgc gggcgccaat tcgctgatga cgggcaacta
     6301 cctgaccacg accggccgcg acagccggct ggaccacgaa atgatcatcg atctggatct
     6361 tgtccccacc cggccgctgg acatctgcaa atgcggccaa gattaaagat ttgacccctt
     6421 gcctttgccg atatcctgca gctgctcgaa ttccatcacg gtggacatgg cgcgattatc
     6481 cactggaatg ccagcttcgc tgactgccac aaccgggttt aatccgccaa tcatgatcat
     6541 tccgatcttg ttcagatcaa catgcgtctg gcagatggga tgcccaatgt ccccgatgga
     6601 taagacggcg ttaatgccgg cgcgcgtgaa atcatcaatc acgcaattca ccagtgtgcg
     6661 gctcaaggcg gggatttcac ggaaattcgc cagaatttta ccatcgccgt tcgccaccac
     6721 ttgcccgatg gaggtcatct tgccgcgaat aaaaatttcc gacgggtcca gcgatgagct
     6781 ggcgtaatga atcagctcca caaatcgcag gccctggcct tttctcagtt gaaggattcc
     6841 gccaaatttg gaatcaacgg gaatgccgtg cttgagcagc acgccgttga ccacaatgct
     6901 gcaaatcgtc gccagacctg ttttcccctc tggcacgacg acgtctccca gcgattctcc
     6961 cgccttcgcc acggcaaccc gtgaactgac agcgagattt ttggcgaaga cgggcctcat
     7021 gattttcagc gcccgggcaa actgcttttc cgggaagaag gaaacattga cgggaaccat
     7081 accgcgcctc tttttaacat cgaaggtggt tcgaaaggcc aggacttcga tgcgtgaaat
     7141 ggaaaggtcg acgttgtcct gagcgcgtgc gttggataca tccgacgacg cggaataagg
     7201 cgagtcatac atcaatgaga cctcactgaa cccggtcatg aagctatcct ttttaaacgg
     7261 catccatcgg cattcgcgcg ctcacccctc ctgacggggc aagcgcgttt caccgatatc
     7321 cttgcatgat gtattattct taatctccgg ccggcggcta tttgagaatt ttttcagctt
     7381 ctttcttatc gacatccata atatactgaa tgccgctgac atccgccgct tcacgggtta
     7441 gtgccgccag gtcgtcccgc gtcatatatt tcagcgcaaa ctttctgctt cccgccatca
     7501 actggcgaag cccctgcgcc agtctttcat agtaggtgta aagtccgatg gcgccggtgg
     7561 gcaactgttc aaattccttg tggcccagtt catggcgcag ctcgctggcc gtgacgaaaa
     7621 tttcatcctt cgtattgccg aagcgttcga cataaaccgg cacctgctgg gcgtcgatgg
     7681 tctggccgat cgtcttaccg accatggccg ccgcaatggg cgcccgcgcc atgccgatca
     7741 atttgacaaa gggcgcgccc atcgccagac ctttgaatat ctgatcctcg aaggtaaagc
     7801 cgccgtttac ggcaatggcc ggcagatatt ttttcttcgc tgcgagcttt ttggcgtatt
     7861 gatacaccag cgtatgcagt tccaccgggg ggacacccca ctcgttcatc atccgccacg
     7921 ggctcatgcc cgtaccgccg ccggcgccgt ccacggtcag cagatccagc ttgtatttcg
     7981 acgaaaaata aatagcccgc gccagatcga cgggacggta ggcgccagtt ttaaggaaaa
     8041 tgtacttggc gccggccttg cgcagcctct taatcgtcgc cgcaaacgat tcctcggtga
     8101 ccatgccgac gcgcgaatga cgctcgaact ccttgaaggc gccccgggca aaggcttcga
     8161 tgacattgga atcataggga tccggcaaaa cgatatagcc gcggtcgtgg agcatctggg
     8221 cttttttcaa atccttgatt ttgacttcgc cgccgatgtc ttttgcaccc tgcccccatt
     8281 taagctccac gcattccacg cccagttcct caatggcgta ttcatgcaca ttcaaccggg
     8341 tatcctccag attggcctgc acgatgatgg cgccgtagcc gaagcgctga taatcccggt
     8401 aaagcttgac gcggcgcttc aaatcgaccg tatccaagat gttgccgttt ttgaatttgg
     8461 attgcgggtc catcccgacg acgttttccc cgatggtcag ccccgttccc gccagggcgg
     8521 acccgatggc cagtccttcc cagttgttct tggcgatgtt ggtggagccg atgccggaaa
     8581 tcagccaggg aagcttgaac tttattccct tatcgtggcc gaaaaccact tccaggttga
     8641 ccgcggggaa aatggccttg tcgctgtcgg cttcaatgcc gtaagcgccg gcggccgtcc
     8701 ccataatgtt gaaatgggaa taatccaccg gatatttctt ttcgccggcg caggtaatca
     8761 cgccgaaagg ctgggggtag atcacttcat ggccgcgata agcggatttg cctatttcac
     8821 acatgccgac gcagccgtcg atgcacgtga cacacatgcc cgacgtcggt gatatagact
     8881 cttctgtcct gtttttggta agggtggcgc tggaggtatt tactcttgaa aaagacatat
     8941 atcctgctcc tttctgggtt taagcttatt ttatttcaca ggccacgcag ggtgacatgt
     9001 aacacatcat atcgtggaac tgatccttc
//
LOCUS       DAOPAF010000064         8913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_7368_length_8913_cov_59.655904, whole genome shotgun sequence.
ACCESSION   DAOPAF010000064 DAOPAF010000000
VERSION     DAOPAF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_8913_cov_59.655904"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PLQ77_06535"
     CDS             complement(<1..1111)
                     /locus_tag="PLQ77_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPL10368.1"
                     /translation="MEKNIVEKILAEHLVDGNLAPGGEIGIRIDQTLTQDATGTMAYL
                     QFEAMNVPRVRTELSVSYIDHNTIQIGFENADDHRYLMSVAQKFGIVLSRAGNGICHQ
                     VHLERFGRPGKTLIGSDSHTPTCGALGMIAVGAGGLDVAQAMAGEPFYLACPSVVKIN
                     LEGRLRPWVSAKDVILKVLEIFSTKGNVGTVFEYGGEGAAALSVPERATIANMGAECG
                     VTTSVFPSDEMTRRFLRLQDREAHFVPLSADADASYARVVTIDLSRLEPLAAKPHSPD
                     NIAAVRSLTGISVQQVCLGSCTNSSYKDLVTVAGILKGRVAHPDVSFILAPGSRQVLD
                     NLARDGYLADLIASGARLMENACGFCIGNSQSPQSG"
     gene            complement(1299..2240)
                     /locus_tag="PLQ77_06540"
     CDS             complement(1299..2240)
                     /locus_tag="PLQ77_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPL10369.1"
                     /translation="MMQRRTIVSWGLFALFLLVCFHIDAHAVQYTVKKGETLSLISKR
                     TGVSVAEIKKANHLTGNTIKRGQILSIKEKKSPSPVTAKSKIQPTPSFYKVKRGDTLP
                     LIAQKTGVPVTQIAKLNHVTTRTLRAGQKLMLPKEEPIANALPEEEDDLLDEEDDGLT
                     DDLSGAIACEDKAGEELLGKWGSAEERKLFIRVATGFLGAPYRLGGATVKGIDCSAFV
                     RKMYEFFDITLPRTAREQSTVGMQVKRDELVEGDLVFFRTKKPIGHVGIYIGNNEFVH
                     ASYKAKAVRIDSLDRPYFQKRFQRAVRLKGLDENGGT"
     gene            2495..3334
                     /gene="mtgA"
                     /locus_tag="PLQ77_06545"
     CDS             2495..3334
                     /gene="mtgA"
                     /locus_tag="PLQ77_06545"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPL10370.1"
                     /translation="MQKWKKIILWSFLAVAVFITLDVGRYFFYPDVAGLQKDRPGKTA
                     FMEYREEIWKEKGINKKISVSWVPLSAVSPYVMKAVIIAEDDKFWSHEGFDFDAMQKA
                     LEKDIKKKKFKAGGSTISQQLAKNLYLTPAKNPVRKIKEAILTWRIERQLSKRRILEL
                     YLNVAEWGDGLFGVGTAARRYYGKSAAGLGPREAAHLAAALPNPNRFRPGGSSRYAAN
                     RAERIYQIMVRRGIVIEEYDEVLKDEGGEEEKAPEPVSAEQEASVPVEPAQTEAPEPA
                     VLP"
     gene            3382..4350
                     /gene="queG"
                     /locus_tag="PLQ77_06550"
     CDS             3382..4350
                     /gene="queG"
                     /locus_tag="PLQ77_06550"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPL10371.1"
                     /translation="MKAASTGKNFQSTDRGDPATLAAAIKEEARRLGFDGCGISRAEA
                     LPEDALRLCSWLERGHHAGMAYMERQPEKRVDPTLLFQGARSVVSVLLNYYPSGTQTD
                     PEAPVLAKYAYGKDYHVVVREKLNRLLKKISSGIAPVRGRAFVDTAPLLEKAWAARAG
                     LGWIGKNTCLISPGKGSFCFIGTLLIDIPLQYDRPVADLCGDCTLCIDACPAKAIVAP
                     RTLDAGHCISYLTIESRPENSGMPNHRLAGRVFGCDICQDVCPWNRSAAPHHEKDFDA
                     PPGLIEMTREDWFNLNEEKYKRILQHSAVQRIGFEGLKRNLERIAG"
     gene            complement(4458..4982)
                     /locus_tag="PLQ77_06555"
     CDS             complement(4458..4982)
                     /locus_tag="PLQ77_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPL10372.1"
                     /translation="MNKEEMLEEVFKRAKACELRGGNCAQCSLNAIFEVLGVEDENVV
                     RSATGFADGVGLSGDGHCGALSGGTMAISYFFGRKKEDLGRMGKQIRALLLAKKFHEQ
                     FVKEFSTCRCHDLQVRQFGRFFNLYDMQDMKAALAAGMPEGCSTLVGKAARMALAIIL
                     DEQARGDLGSNLYS"
     gene            complement(5198..6373)
                     /locus_tag="PLQ77_06560"
     CDS             complement(5198..6373)
                     /locus_tag="PLQ77_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPL10373.1"
                     /translation="MTYIRHIEHENIVHRCFRCGYCKFPSDYSDFNCPSYKAFGWDTF
                     SPGGRMWLTRAWLSGEIETSARFAEIMFSCVACDNCKDQCVFPRFKDFLPDIFQETRA
                     ELVGEGRIPPGVRDYFKAVAISGNPYKLPQSERGDWARGTGLKPFTNQKHLFYAGCVG
                     SYDEVGKRMAQSVGKMLVDAGLSVGILGSEETCDGNDVRVLGEMGLFAELAQANIEKF
                     KAKGVKEIITLDPHALNTFKKDYPALGGVFQVRHYSEVLAELVKKKKIKLKAYPVKIT
                     YHDPCYLGRHQSIYQAPREILKAIPKAEFVEMHRSGPDAFCCGGGGGNFFTDILGTAE
                     DSAARVRVREAAETGARILAVACPNCAKMLSDAVKMENLEDRLELLGIAEILERVRK"
     gene            complement(6370..7752)
                     /locus_tag="PLQ77_06565"
     CDS             complement(6370..7752)
                     /locus_tag="PLQ77_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPL10374.1"
                     /translation="MSDIRKSLVEIVGERGVSDAPEELWFYSRDPGVLAPHRPDYVVA
                     PKTTEEVQKIVQLAGRENIPVVPMGNGMSLAGLVIPLKGGIVLDMKRMNKILEVNHMA
                     RYVVVEGGTSQGALKAYLQKNHPTLRHSIPDAPSTTTIAANVSLHGQGRLTNQYGFNS
                     DMVTGLEVVLPTGEICRIGSPSIGPYWISKGPTLPDLSGLFLGWLGCTGVITKVGLKL
                     YPNKKIRDAELFVTDRVDLIPHILYKLTHLEMLEDINVWFQPKPLMFMGNYHITVFFT
                     GDTEEEVEFKRKMVWDCLQEYMDSKDGGFMSIQYMKSMLLEMPGRNIADFADVPKGGG
                     FEYSGPIAPIEHFPQYAAKVVELAAKYNVLYASTARLISGGHSMMYSISFAFNRSDTA
                     MMERVEQALTEATAFALDMGGIPWKPNFKEQKMTLEKMEPNARKLLLMIKKNLDPQGI
                     MNPGNWEVNS"
     gene            complement(7749..8783)
                     /locus_tag="PLQ77_06570"
     CDS             complement(7749..8783)
                     /locus_tag="PLQ77_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPL10375.1"
                     /translation="MIQLKFDQKVCASCRSADCLLKCQYMTFSGHKEAHGEMMKVMKG
                     KDSRVLTECVTCYACEEYCTRGNHPFYLISEKREEKGMFTAPRPITTQWKNMTSMQGK
                     YLVGNVKDTALSCCYLPELGQLGTGEIFKDVASAQVFGAEFMCPAVHSHFARMSVIKE
                     RLPMVIENYRKLGVKEVICMHDECYGTLTSIASAYGIEVPFTPIYYLDFVLQRMKELK
                     DRIKPLGITVAYQRSCSNRLIPDKLPVVKKIFQLIGVKAPKRKYQGENALCCAEIIRS
                     VSGYKLADDVQKRNIDDMLAAGAEYCVFNCPACQTSLSEKVTRRGLKPVHIIDLCKMA
                     IGEKEREAVS"
BASE COUNT         1877 a         2416 c         2585 g         2035 t
ORIGIN      
        1 cgccggattg cgggctctgg ctgttgccga tgcagaagcc gcaggcgttt tccataagcc
       61 gcgcgccgga ggcgatcaga tcagccaggt agccgtcgcg ggcaagattg tcgagaacct
      121 gccgcgagcc cggcgctaaa atgaagctga catcgggatg cgcaacgcgg cctttgagga
      181 ttccggcgac cgtcacgaga tccttgtagg aagagttggt gcagcttccc aggcagacct
      241 gctgtacgga gatgccggtg agcgaccgga ccgccgcgat gttgtcgggg ctgtgcggtt
      301 tggctgcgag cggctcgagc cgggacaggt cgatggtcac gacgcgggcg taagatgcgt
      361 cggcatcggc gctcagggga acgaagtgtg cctctctgtc ctgcaggcgc agaaaccggc
      421 gggtcatctc gtcgctggga aacacggagg tcgtcacgcc gcattccgcg cccatgttgg
      481 cgatggtggc gcgctccgga acgctcagcg ccgctgcgcc ttcgccgccg tattcgaaaa
      541 cggttccgac gtttcccttg gtggaaaaaa tttccagtac tttgaggatg acgtccttgg
      601 cggaaaccca ggggcgaagc cggccttcga ggttgatctt taccacggaa gggcacgcaa
      661 ggtaaaaagg ctctcccgcc atcgcctgcg cgacgtccag tccgccggcg ccgacggcga
      721 tcatgcccag cgcgccgcag gtgggcgtgt ggctgtcgga gccgatcagc gttttgcccg
      781 gacggccgaa gcgctccaga tggacctggt ggcaaattcc gttcccggcg cgcgacagaa
      841 cgatgccgaa cttttgcgcg acgctcatca ggtaccggtg atcgtcagcg ttttcaaagc
      901 cgatttgaat cgtgttgtgg tcgatgtagc tcaccgacag ctccgtgcgg acccggggaa
      961 cgttcatggc ttcaaactgc agataagcca tggtgcccgt ggcgtcctgg gtgagggtct
     1021 ggtcgatgcg gatgccgatt tccccgccgg gcgcgaggtt gccgtccacc agatgttcgg
     1081 ccagaatttt ttccacgatg tttttttcca ttggttcttc cttgcggcac attgcgatta
     1141 ttccagttga ttcagcagtc tttcacccat tcgatacggg aaccggcagg attgtcctca
     1201 tataataaac ggccgggata agcaaccggc aaaacggcca ataaaaaaaa gaggcgttca
     1261 agcctctttt tgaatacctt ttgagtcacg aaacacaatc aggtgccgcc attttcatcc
     1321 aggcctttga gacgtacggc ccgctgaaat ctcttctgaa aatagggtcg atccaggctg
     1381 tcgatgcgga cggcctttgc cttgtaagag gcgtgaacga actcgttgtt gccgatgtaa
     1441 atgccgacgt ggccgatcgg ctttttggtc cggaagaaaa ccaggtcccc ttccaccagc
     1501 tcatcacgct tcacctgcat gccgactgtg gattgttccc gggcggtgcg gggcagagtg
     1561 atatcgaaaa attcgtacat tttccggaca aaggccgaac agtcaattcc cttgacggtg
     1621 gcgcctccca gccggtaagg tgcgcccaga aagcctgttg ccactcggat gaacagtttt
     1681 ctttcttcgg cgctgcccca tttgcccaaa agttcctcgc ctgctttatc ttcacaggcg
     1741 attgcgccgg agaggtcatc cgtcaatccg tcatcttctt cgtccagcaa atcgtcctct
     1801 tcctcgggga gcgcattcgc gatcggttcc tctttgggca gcatcagctt ctgtccggcg
     1861 cgcagggttc tggtggtgac atgattcagc tttgcgatct gcgtaacggg aacgccggtt
     1921 ttttgggcaa tcaggggaag cgtgtcgcct cttttaactt tatagaagga tggagtgggt
     1981 tgaatttttg attttgcggt gacgggggat ggagattttt tttctttgat gcttaaaatc
     2041 tggccgcgtt ttatggtatt gccggtcaaa tggttggctt ttttgatttc cgccacactg
     2101 acgccggttc gcttggaaat gagggagagg gtttcacctt ttttaacggt atactggacc
     2161 gcatgggcgt cgatatgaaa acagaccagc aggaaaagag caaataatcc ccaagatact
     2221 atggttcgcc gttgcatcat gaagtacctc ctgtttagcg gggggagccc catattgtta
     2281 acggtgcaca gattgtggat cggtgaagct actatttgta tatcaaagat attttactgc
     2341 acacagaaaa cgaaagttta caattaaacg acagcttatc aacttcgtac gagtccccct
     2401 tactaaaaat ggaagggcat gtcaaattta ttttgcaatc tttttcaatg aggtatagga
     2461 agcggcaaac gaaaaacaag aggaaagtgc gctcatgcaa aagtggaaaa agataatttt
     2521 atggagtttt ttggctgtgg ccgtttttat cacgctggat gtcggccgct atttttttta
     2581 tccggacgtg gccggtctgc agaaggatcg tcccggcaaa accgccttca tggaataccg
     2641 ggaagagata tggaaggaaa aaggcattaa caaaaaaata tccgtatctt gggtgcccct
     2701 gtcggccgtg tcgccttatg taatgaaggc ggtgattatt gccgaggatg ataaattctg
     2761 gtctcacgag ggatttgact ttgacgcgat gcagaaggcg ttggaaaaag acatcaaaaa
     2821 gaagaaattc aaggcgggag gcagtacgat ttcccagcag ctggcaaaga atttatatct
     2881 gacgccggcg aaaaatccgg tgcggaaaat caaggaagcc attttaacct ggcgaatcga
     2941 aaggcagttg agcaagagaa gaattctgga gctctatttg aacgtggccg aatgggggga
     3001 tggcctgttt ggtgtgggga ccgccgcgcg cagatattac ggcaaatccg ccgccggcct
     3061 cggaccgcgt gaagcggccc atcttgcggc ggctctgccc aatccgaacc gttttcggcc
     3121 gggaggcagt tcaagatatg cggccaaccg ggcggagaga atttatcaga tcatggtgcg
     3181 gcgcggcatt gtcattgaag agtatgatga agttctgaag gatgaaggcg gagaagaaga
     3241 aaaagcccct gagccggtat cggcggagca ggaagcctct gttcccgttg agccggcgca
     3301 gacggaagcg cccgaacctg cggttcttcc ctgacagtct tgaaaccgcg ggcgtcagga
     3361 ttggaaaacg ggataaaatc aatgaaagca gcgtccacag gaaaaaattt tcaatccact
     3421 gaccggggtg atccggcaac gctggccgcc gcgatcaagg aggaggcccg ccggctcggg
     3481 tttgacggct gcgggatttc ccgcgccgaa gcgcttccgg aagacgctct ccgcctgtgc
     3541 tcttggctcg aacgcgggca tcacgccgga atggcctaca tggaaaggca accggagaag
     3601 cgtgttgatc cgaccctgct gtttcaaggt gctcgctcgg ttgtttccgt tcttttgaat
     3661 tactatccgt caggaacgca aaccgatccg gaagcgccgg ttttggcaaa gtacgcctac
     3721 gggaaagatt atcacgtcgt tgtcagggaa aaactgaacc gtctgctgaa gaaaatttca
     3781 tccggcatcg cgccggttcg cggccgggcg tttgtggata cggcgccgct gctcgaaaag
     3841 gcgtgggccg cccgggcggg gctggggtgg atcggcaaaa acacctgcct gatttcaccg
     3901 gggaaaggct ctttttgttt cattggaacg cttctgatcg atattcccct gcaatacgac
     3961 cgtcccgttg ccgatctctg cggcgactgc acgctctgca tcgatgcctg ccccgcgaag
     4021 gccattgtcg ccccgcggac gctggatgcc ggccactgca tttcctacct gacgatcgag
     4081 agccgcccgg aaaacagtgg gatgccaaac catcggttgg ccggccgcgt gttcggctgc
     4141 gatatctgcc aggatgtctg cccctggaac cgcagcgccg ccccgcacca tgaaaaagat
     4201 tttgacgcac cgcccggctt gattgaaatg acccgcgagg actggttcaa cctcaacgag
     4261 gaaaaataca agagaatatt gcagcactcc gcggtgcaaa gaatcggatt tgaggggctg
     4321 aaaaggaacc tcgagcgcat agcgggataa tccggtcacg ggaagactgt gtttttttcc
     4381 agcgcggggg atatttttga aatatttacg caaagccagt cgacgactgg tgaaaacgga
     4441 agcgtgacgg tcaaatgtca agaataaaga tttgacccca agtccccgcg ggcctgttcg
     4501 tcgaggatga tggccagcgc catgcgcgcc gccttgccca cgagtgtgga gcagccttcc
     4561 ggcattcccg cggccagcgc cgccttcatg tcttgcatgt cgtagaggtt gaaaaagcgg
     4621 ccgaactgcc tgacctgaag atcgtggcag cggcaggtgg agaattcctt tacgaactgt
     4681 tcatggaatt ttttggcgag cagcagcgct ctgatctgct tgcccatccg gcccaggtct
     4741 tcttttttcc ggccgaaaaa gtagctgata gccatcgtgc cgccggaaag ggcgccgcag
     4801 tggccgtcgc cggaaaggcc cacgccgtcg gcaaatcccg tggcggagcg cacgacgttt
     4861 tcatcctcca cgcccaagac ttcaaaaata gcattcagcg agcattgcgc gcagttgccg
     4921 ccgcgaagtt cacaggcttt cgccctctta aagacttctt cgagcatttc ttctttgttc
     4981 atttgattat gatctccgtt attatttttt caggcttaat attccttaga tgcttccccg
     5041 cccctggtgg gcggggactg gggggagggg gatttgattt caccctcacc ctaaccctct
     5101 cccatcgaag ggagagggta ccaaaggcag ttcctgacat acaggcgcag agttgccgtt
     5161 tttccgcaac tcccatcgaa gggagaggga accaaagtca tttccggacc cgctccagaa
     5221 tttcagcgat gcccagcagc tcgagtcggt cttccagatt ttccatcttc accgcgtcgg
     5281 agagcatctt ggcgcagttg gggcaggcga ccgccagaat ccgagctccc gtctcggcgg
     5341 cttcgcgcac acgcacgcgg gcggcgctgt cttcggccgt tcccagaatg tccgtgaaga
     5401 aatttccgcc tcctccgccg cagcagaaag catcgggacc gctgcgatgc atttcgacaa
     5461 actcggcttt gggaatagcc ttcaggattt cgcgcggcgc ctggtaaatg gactgatgcc
     5521 ttcctaggta gcagggatcg tggtaggtga tcttgaccgg atacgctttg agcttgattt
     5581 ttttcttttt caccagctcg gctaaaacct cgctgtagtg acggacctgg aagactccgc
     5641 caagggcggg gtagtctttt ttgaacgtat tgagcgcatg cggatcgagc gtgatgattt
     5701 ctttgacgcc ttttgccttg aatttttcaa tgttggcctg ggcgagctcg gcaaacagtc
     5761 ccatctcgcc cagaacccgc acgtcgttgc cgtcgcaggt ctcttcgctg ccgagaatgc
     5821 cgaccgacag gcccgcatcc accagcattt ttcccacgct ttgggccatg cgctttccga
     5881 cttcatcgta ggagccgacg cagcccgcgt aaaagaggtg cttctggttg gtaaaaggct
     5941 tcagtcccgt cccgcgggcc cagtcgcccc gctcgctctg cgggagcttg tagggattgc
     6001 cgcttatcgc gacggccttg aagtagtcgc gcacacccgg cggaatccgt ccttcgccca
     6061 ccagttcggc gcgggtttcc tgaaagatgt ccggcaggaa atccttgaac ctgggaaaca
     6121 cgcactgatc cttgcagttg tcgcaggcca cgcaggaaaa cataatttcc gcaaagcgcg
     6181 cggaggtttc aatttcgccg gacagccacg cgcgggtgag ccacattcgg ccgccgggcg
     6241 agaaggtatc ccagccaaag gccttgtagg aagggcagtt gaagtcgctg taatccgaag
     6301 ggaacttgca gtagccgcag cggaagcagc gatggacgat gttttcatgt tcgatgtgcc
     6361 gaatgtaggt catgagttca cctcccagtt gccggggttc atgattccct gcggatcgag
     6421 atttttcttg atcatcaaca ggagcttgcg cgcgttgggt tccatttttt caagcgtcat
     6481 tttctgctcc ttgaaattgg gcttccaggg aatgccgccc atgtccaggg caaaagccgt
     6541 cgcttcggta agcgcttgct ccacgcgctc catcatggcg gtgtcgctgc ggttgaaggc
     6601 aaaagagatg ctgtacatca tggagtgccc gccggaaatg agccgggccg tgctggcata
     6661 gaggacattg tatttggcgg ccagctccac cacctttgcg gcgtattgcg gaaaatgctc
     6721 gatcggcgcg atgggccccg agtattcaaa cccgccgccc ttcggcacat cggcaaaatc
     6781 ggcgatgttg cggcccggca tttccaggag catggacttc atgtattgaa tcgacatgaa
     6841 gccgccatct ttggaatcca tgtattcctg aaggcagtcc cagaccatct tccttttgaa
     6901 ttcgacttct tcttccgtgt cacccgtgaa gaaaaccgtg atgtggtaat tccccataaa
     6961 catgagcggt ttgggctgga accagacgtt gatgtcctcc agcatttcca ggtgggtaag
     7021 cttgtagagg atgtgcggaa tcagatcgac ccggtccgtg acgaagagtt ccgcgtcccg
     7081 gatctttttg ttggggtaga gcttcaggcc caccttggta atgacgcctg tgcagccgag
     7141 ccagcccagg aagagccccg acaaatcggg aagcgtaggc cctttggaaa tccagtaggg
     7201 gccgatcgac ggcgaaccga ttcggcagat ctcgcccgtg ggcaggacca cttccagccc
     7261 cgtgaccatg tcggagttga aaccgtactg gttggtgagc cttccctggc cgtgcagcga
     7321 aacgttggcg gcgatggtgg tggtcgaagg ggcgtcggga atgctgtgcc ggagcgtcgg
     7381 atggtttttc tgcagatagg ctttgagcgc gccctgggaa gtgccgcctt ctacgaccac
     7441 gtagcgcgcc atatgattca cttccaggat tttgttcatc cgcttcatat ccagaacgat
     7501 gccgcccttg agcggaatca ccaggcccgc gagcgacatg ccgttcccca ttggaacgac
     7561 ggggatattt tcacggcctg ccagttgaac aatcttctgg acttcttccg tggttttggg
     7621 cgcgaccaca tagtcgggtc tgtgaggcgc gagcaccccg ggatcgcgcg aataaaacca
     7681 gagttcttcc ggagcgtcgg agacgccccg ctcgccgacg atctccacga gggattttcg
     7741 aatatcgctc atgagacagc ctccctttct ttttcgccga tcgccatctt gcacagatcg
     7801 atgatgtgca ccggtttcaa tccccgcctg gtcacttttt ccgacagaga cgtctggcag
     7861 gccggacagt tgaaaacgca gtattcggcg cccgcggcca gcatgtcgtc aatgttgcgc
     7921 ttctgcacgt cgtcggccag tttgtagccg gaaacggagc ggataatttc cgcgcagcac
     7981 agcgcgtttt cgccctgata cttccgtttg ggcgctttga cgccgatcaa ctggaagatt
     8041 tttttcacca ccggcagttt gtcgggaatg agccggttgg agcaggagcg ctgataggcg
     8101 acggtgatgc cgagcggctt gatcctgtct ttgagttctt tcatgcgctg caaaacaaaa
     8161 tccagataat aaatcggggt gaagggcacc tcgatgccgt aggcggaggc aatcgaagtc
     8221 agcgtgccgt agcattcgtc atgcatgcag ataacttctt tgacgcccag cttgcggtag
     8281 ttctcgatga ccatggggag ccgctccttg atgaccgaca tgcgggcgaa gtgggaatga
     8341 accgccgggc acatgaattc cgcgccgaac acttgggccg aggcgacgtc cttaaagatc
     8401 tctcccgttc ccagttgccc cagctcggga aggtagcagc aggaaagcgc cgtatccttc
     8461 acgttgccca ccagatactt gccctgcatc gacgtcatat ttttccactg cgtcgtgatg
     8521 gggcgcggcg cggtgaacat gcctttttcc tcacgcttct ccgagatgag gtaaaaggga
     8581 tggttgcccc gcgtgcagta ttcctcgcag gcgtagcagg tgacgcattc ggtcagcacg
     8641 cgggaatcct ttcccttcat gactttcatc atttccccgt gcgcttcctt gtgcccgcta
     8701 aaggtcatgt actggcattt gagcaggcag tcggccgagc ggcagcttgc gcaaaccttc
     8761 tgatcgaatt tcagttgaat catgaagcac ctccctcgat gaatgatggg atccataaaa
     8821 cccgatttga cgcccgctcc tgtccggcag gggctggacg ctttatttag ccatgctttc
     8881 cagcatgatt tccgcggcaa tcctggcgcc cat
//
LOCUS       DAOPAF010000065         8538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_7719_length_8538_cov_35.449841, whole genome shotgun sequence.
ACCESSION   DAOPAF010000065 DAOPAF010000000
VERSION     DAOPAF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_8538_cov_35.449841"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..855
                     /locus_tag="PLQ77_06575"
     CDS             274..855
                     /locus_tag="PLQ77_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPL10376.1"
                     /translation="MKQEPDFIEADIGKRIKSLRNKKRITLESLAAETGFTKGYLSKV
                     ETSEKAPPVSTLGSIARALGVSISTLLGEESPIQSICLVKKSERPLITRNGSNAGYSY
                     EAVAHKFKNKVMDPFVLTLPVQPKKRILYQHEGQEVIFVLEGTMSFRHGEEELIAEEG
                     DCVYFDSSIPHSGVSLGDKAVKCFMVIFNPGNQ"
     gene            927..1790
                     /locus_tag="PLQ77_06580"
     CDS             927..1790
                     /locus_tag="PLQ77_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL10377.1"
                     /translation="MSDIPVFFLHGLAYAGLLFLVSSGLTLIFGMMNVLNFAHAAMYM
                     IGAYLSYTIIMQMTGQFWMALMICPVILFIIGALVERFLLRRVHAQGHLGELLLTFGL
                     AYIITELVKWHWGNYPLAVNVTGFLNGTITIFGFIYPIYRIFIFVAAVLVGGVMALIL
                     YKTRLGIILRASVNDSEMTNILGVNVPLVFMGVFAFGTALSGFAGVIAGPLLSTFPGM
                     AQEILIDAFVVIVVGGFGSLGGAVVAALLIGQLQSFGVLLIPKLSVALVYLLMAAVLI
                     IKPSGLFGEES"
     gene            1793..2797
                     /locus_tag="PLQ77_06585"
     CDS             1793..2797
                     /locus_tag="PLQ77_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPL10378.1"
                     /translation="MKQRNISIIAIVVLFLAGLLMPFVLKQYHLNMLTEIIIFSLYAI
                     SYNLLLGYAGLLSFGHAAFFGLGAFVTAVAVIHIPGISLWAVLLIGVAAAVLAGLVLG
                     SLLLRHKGSYFALLTLAFNALFYAVATKWHSVTGGDDGLSITRPDLPLGFTTVPLSGL
                     TPFYYLTLVIMGAAIVYCWYFTNTAMGKTVLLMRENEERMKFLGFNTNISRLILFTFT
                     GAMAGLAGVFYTFHFQFVSISAISVDMTTAVLLMVFVGGTKKFWGPILGVFVYIIFQN
                     YLSNITDRWPLFMGLIFVLMVLYVPGGLSGVMMDVWQRLFGKKDPNGGKSAAAEEATP
                     "
     gene            2794..3537
                     /locus_tag="PLQ77_06590"
     CDS             2794..3537
                     /locus_tag="PLQ77_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10379.1"
                     /translation="MSDYLHIQNIHKDFSGLRVLTGVEFKVTEKERHAVIGPNGAGKT
                     TLFNIISGKFKPSSGAILFKGKDISGKPPHVLNRHGLARSFQITNVFQELTVFNNVLA
                     GVRSRYGLRYNFFKQPNRNREICEKVEAIIDKVGLTDVKDLPASSLSYGQQRALEIGV
                     TLSSEPELILLDEPTAGMTREETERAIKMIDQVTAGRTLIIIEHDMEVVFSLADTISV
                     LHYGTILVSDTPEKIRNDQRVKDAYLGEG"
     gene            3540..4250
                     /locus_tag="PLQ77_06595"
     CDS             3540..4250
                     /locus_tag="PLQ77_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10380.1"
                     /translation="MLEVRNIHSYYGKSHILHGVSLDLKEGEMVCLLGRNGVGKSTTL
                     KSIMGVVKPEQGSIRFHDREIAGLAPYEIARLGIGYVPEDRRIFRSLTVHENLLMGIK
                     RGKNKNAGAEEWTVERVYKLFPKLEERRNNKGSHLSGGEQQMLTVVRTLMGNPRVILV
                     DEPTEGLAPLIVKDVLEMLTTVRKSGVTVLMVEQNFKAAVKVADRYYIMSKGQIVFAG
                     NTQELLAADEVRKNYLEV"
     gene            4377..5591
                     /locus_tag="PLQ77_06600"
     CDS             4377..5591
                     /locus_tag="PLQ77_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL10381.1"
                     /translation="MKKKMVYGLCFLSMVLMLAMPIVVSAAEDTIKLGVVEPLSGTFK
                     DIGERYLDGAEYAVRVINESGGLLGKKVEVVRIDSELKPDVATRKAQNLILRDGVKFF
                     CGGTGSSVGAAMEQLAEKQNVIMFTYGMDAASMTGVKCNKNFFRPGGSTDGRSFAIAQ
                     QIAKLGYKKVAIIAQDYSFGQEALAAFKKEMARISPQTKIVAELYHPAGTKDYGPYAS
                     QLIAAKPDVIFTPNWGNDLTLLLKQGRPMGMKQKVFSYYINDEVTIESLGNDSLMIGN
                     MGAEIYTLSIPTKKNEEFVAKFYKDKGYYPTWLRGKAYIATMFWAEAVKKAGTTDVAA
                     VIKAWEGLSYDGPAGVWTMRACDHQAQVPYWYTEIVAKTKYFKHAFEAPRGMTPAKAV
                     EVPCADTGCKMK"
     gene            5759..6808
                     /locus_tag="PLQ77_06605"
     CDS             5759..6808
                     /locus_tag="PLQ77_06605"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10382.1"
                     /translation="MAEVSGKVMSAKEAVDRFVHNGDELVIGNYTVGSCAELVYEIAR
                     QRKKGFTLYSQSGIFDVEVLVAGGCVDRLVTTYVMRSGGKKGGSAVERALNAGTLEIE
                     DYTNFEYNARLVAGMHGFSFMPVLEGVMVTDIFRRRGFMGENKFRVIQCPYTGKDKLT
                     VPAAAPDVCVVHVQRADAEGNAQYWGAMGSVAAAALASKRIIVSCEEIVDHEIIQSSP
                     HLTIIPAYRVNAVVEAPWGAHPTEVLGYYNVDTFMYNGLFSTAAATADGLAAWMEEWV
                     YGCPDRARYIDHYIARFGSEALDELRAKPFYSAPADYGAAFSSHWDREGRERSMGVTL
                     PELEKLLKDRGKVYG"
     gene            6801..7649
                     /locus_tag="PLQ77_06610"
     CDS             6801..7649
                     /locus_tag="PLQ77_06610"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10383.1"
                     /translation="MGKPYTLDELLAIAVAREIHDYENVVLGVGVPMTAGALAKSLLA
                     PHATLMMESGIVDIRPLLSLNNIADAHSARGFSYATDLFSAFTATYRGFVDVCFLGVA
                     QVDKYGNLNTTVIGDYNKPDMRLPGSGGAADFISYARRTVLTMRGGAFVNKLDYFTSP
                     GYLEGGDSRDKTGLFPIGSGPTALISTKGVFRFDATTKEMYLATIHPGVSLESVRKDI
                     PWNLKASPDLSLTERPTDEEIDFIRNFAPELSAGRELTVELRNAAVKKKAELRAGKRP
                     GAGGRL"
     gene            7646..8407
                     /locus_tag="PLQ77_06615"
     CDS             7646..8407
                     /locus_tag="PLQ77_06615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HPL10384.1"
                     /translation="MKLKNKVAVVTGASKGIGKAVALAFAREGASVVPASRTPAALED
                     VVREIRAAGGEASAVSVDVRSVDSINALVKKAVDRYGRLDLLVNNAGITMGGPSEDLA
                     PEDWRAALETDLFGVFYACQAAARVMIPQGGGNIINISSVNGILAAPRRAAYCASKAA
                     VNELTKVLAIEWADRKIRVNAIAPGYVRTELVEDVIHKGAISLEAILKRTPQGRIGEV
                     GDIASLAVYMAGDESSYMTGSIVNIDGGWLAYGYL"
BASE COUNT         1898 a         2233 c         2473 g         1934 t
ORIGIN      
        1 gacggcaacc ctttctgttt tctgaagtat agaaagaacg gagacgggag tcaaggccat
       61 tcagaaattt ccctcaattc ctgcccgccg ggaagtttcc ggagcggacg aaacccggaa
      121 agtattcccc aaaataattc cttgacaaaa gttttctatc atgaaatata tgtttcccaa
      181 aagagcatgc cccgttaagc gctctccttg ctgttgcttg aatgtaagcg ccatgcggca
      241 ttgttttaca ttaaacgaat aaggtttcct gatatgaaac aagaaccaga cttcattgaa
      301 gccgacatcg gaaaaagaat caaatcatta cgcaacaaga aacgaatcac actggagagc
      361 cttgccgcgg aaacgggttt taccaaaggc tatttgtcta aggtggagac gtcggaaaag
      421 gcgcccccgg tctccacgct ggggagtatt gcccgagccc tgggagtcag tatttccact
      481 ctcttgggag aagaaagccc gattcaatcc atctgtcttg ttaaaaaaag cgagaggccc
      541 ctgattacca ggaacggatc gaacgcagga tattcctatg aggcggtggc tcacaaattc
      601 aaaaataaag tgatggaccc ttttgtttta acgcttccgg ttcaaccgaa gaagcggatt
      661 ctttatcagc acgaaggcca ggaggtcatc tttgttttgg aaggaacgat gagctttcgc
      721 cacggcgaag aggaattaat agcggaagaa ggtgactgtg tctattttga ttccagtatc
      781 ccccattcgg gagtatctct gggggacaag gcggtcaagt gtttcatggt cattttcaat
      841 ccggggaacc agtaaccatg cagtcgtttc gccgggagcg gtgatgcgcc cggcttggga
      901 ggcaactgcg gcagaaagga aaaaacatga gtgatatccc cgtctttttt ctccacggtc
      961 tcgcgtatgc cggacttctt tttctcgtct cgtcagggct cacccttatt ttcgggatga
     1021 tgaacgtcct caactttgcc catgccgcca tgtacatgat cggcgcttat ctctcctaca
     1081 ccatcatcat gcagatgacc ggccagttct ggatggcgct gatgatctgt cccgtgatcc
     1141 ttttcattat cggggctctg gtggagcgct ttcttctgcg gcgcgtacac gcgcagggtc
     1201 acttaggcga acttctgctg acgttcggtc tggcctacat catcacggaa ttggtcaaat
     1261 ggcactgggg gaattatccg ctggccgtca acgtgacggg attcctcaac ggaaccatta
     1321 ccatatttgg attcatttat cccatctacc gcattttcat ttttgtcgcc gccgtcctgg
     1381 tcggaggggt gatggccctg atcctgtaca agacccgcct ggggattatt ctccgggcgt
     1441 ccgtcaacga tagtgagatg accaatatcc tgggggtgaa cgttcctctc gtcttcatgg
     1501 gcgtctttgc cttcggcacg gcgctttccg gatttgccgg cgtgatcgcg ggtccgcttc
     1561 tttccacttt cccgggcatg gcccaggaaa ttctgattga tgcctttgtt gtgattgtcg
     1621 tgggcggctt cggaagtctg gggggcgccg ttgtcgccgc gctgcttatc ggccaactcc
     1681 agtccttcgg cgttctcctg atccccaagt tgtcggtggc gctggtttat ctgctcatgg
     1741 ccgccgtgct gatcatcaaa ccatccgggc tctttggcga ggaatcctaa tcatgaaaca
     1801 gcgaaacatt tccatcatcg cgattgtcgt attattcctg gcggggcttt tgatgccctt
     1861 tgtcctgaaa cagtaccatc tgaacatgtt gacggagatc atcatttttt ctctctatgc
     1921 catcagttac aatcttctcc tcggatatgc cgggctgttg tccttcggcc atgccgcgtt
     1981 tttcggtctg ggggccttcg tgacggccgt ggcggtcatt catatccccg gcatatccct
     2041 gtgggccgtc ctgttgatcg gcgtcgccgc ggccgttttg gccggcctgg tcctcggcag
     2101 tctgctgttg cggcataaag gctcctactt tgccctgctg acattggcct tcaacgccct
     2161 cttttatgcc gttgcgacca aatggcactc cgttaccggt ggagacgacg gtttaagcat
     2221 cacccggccg gatctgcccc tgggctttac gaccgtcccc ctgtccggct tgaccccctt
     2281 ttactacctg acccttgtga tcatgggcgc ggcgattgtc tattgctggt attttacgaa
     2341 tacggcgatg ggcaagacgg tcctgctgat gcgtgaaaac gaggaacgga tgaaatttct
     2401 ggggtttaac acgaatatca gccggctgat tctctttacc tttaccggag cgatggccgg
     2461 gctggccggc gtcttctata cgtttcattt ccagtttgta tccatttcgg ccatcagcgt
     2521 cgatatgacg acggcggttt tgctcatggt ctttgtcgga ggaacgaaga aattctgggg
     2581 gcccattctg ggcgtctttg tctatatcat cttccagaat tatctgagta atattacgga
     2641 tcgctggccg ctgtttatgg ggctcatctt tgttttgatg gtcctgtatg ttccgggcgg
     2701 cctgtccggc gtcatgatgg atgtatggca gcggttattc ggaaagaaag accctaacgg
     2761 cggtaaatca gccgcggcgg aggaagcgac accatgagcg attatcttca tatccagaat
     2821 attcacaagg acttttcggg cttgcgggtt ttgaccggcg tggagttcaa ggtcacagag
     2881 aaggagcgtc atgccgtcat cggtcccaac ggcgcgggaa agacgacgct gttcaatatt
     2941 atcagcggca aattcaagcc ttcatccggg gcgatccttt tcaagggaaa ggatatttcc
     3001 ggaaagccgc cacatgttct gaaccgccat gggttggccc gttctttcca gatcaccaat
     3061 gtcttccagg aactgaccgt cttcaataac gttcttgccg gagtgcgctc ccgctacgga
     3121 ttgcggtata atttcttcaa gcagcccaat cggaaccggg agatttgcga aaaagtcgag
     3181 gccatcatcg acaaggtcgg acttacggat gtcaaggatc tgccggccag ctcgctgtct
     3241 tacggccagc agagggctct ggaaatcggc gtcacccttt cgtcggaacc ggagttgatt
     3301 ctcctggatg aacccaccgc gggcatgacg cgcgaggaaa cggagcgggc cattaaaatg
     3361 atcgatcagg tgacggccgg ccgcacgctg atcattatcg aacacgacat ggaggtcgtt
     3421 ttctcgcttg ccgacacgat ttccgttctt cattacggga cgattctcgt ctcggataca
     3481 ccggaaaaaa tcagaaatga ccagcgcgtg aaagacgcct acctggggga aggataaaca
     3541 tgctagaagt caggaatatt cacagctact acggcaaaag tcatattctg catggcgttt
     3601 ctctggattt aaaagaaggg gaaatggtct gtctcctggg gagaaacggc gtcggcaaat
     3661 ccacgacatt aaagagcatc atgggagttg taaagcccga gcagggcagc atccgcttcc
     3721 atgatcggga aatcgcaggc cttgccccct atgaaattgc ccgcctgggc atcggctatg
     3781 tccccgagga ccggcggatt ttccggagcc tcaccgttca cgagaacctc ctgatgggca
     3841 tcaaacgcgg gaaaaacaaa aatgccgggg cggaagagtg gaccgtcgag cgggtttata
     3901 aactttttcc caaactggag gaaaggagaa acaacaaggg gagtcacctt tccggcggtg
     3961 aacagcagat gcttaccgtg gtgaggacgc tgatggggaa tccccgggtt attctggtgg
     4021 acgaaccgac ggaaggactg gcgccgctga tcgtcaaaga cgttctggag atgctgacaa
     4081 ccgttcgaaa atcgggagtg accgtcctga tggtggagca gaatttcaag gcggccgtca
     4141 aggttgcgga ccgctactat atcatgagca agggccagat tgtttttgcg ggaaacaccc
     4201 aagagcttct ggcggccgat gaggtgagga aaaattatct ggaagtatag cgacgccgca
     4261 atccccatgt ctcatccgtg ccggattgct gtagcgcgcg tcatgttggg tcactgcttt
     4321 tatgtgtttg atcagcgtga gaaaaaaaga acaacaaaaa gaaagggagg atgtctatga
     4381 aaaagaaaat ggtttacggg ttgtgttttt tatccatggt cttgatgttg gcaatgccta
     4441 tcgttgtttc ggcagccgaa gacactatca agctcggagt ggtagagccc ctttcaggaa
     4501 cgttcaagga catcggtgaa cgctatctgg atggagcgga atatgccgtc agagtgatta
     4561 atgaaagcgg cgggcttctg ggcaagaaag tggaagtggt ccggatcgat tcggaattga
     4621 aaccggacgt ggcgacccgt aaggcgcaga acctgatttt gagagacggt gtgaagttct
     4681 tctgcggcgg cacaggcagc tcggtgggtg ccgccatgga gcagctggcg gaaaaacaaa
     4741 acgtcatcat gtttacctac ggcatggacg ccgcctccat gaccggcgtg aagtgcaaca
     4801 agaatttctt ccggcccggc ggcagcacgg acggccgttc tttcgccatc gcgcagcaga
     4861 ttgccaaact gggttacaaa aaggtcgcga tcatcgccca ggattattct ttcggccagg
     4921 aagcgctggc cgctttcaag aaagagatgg ccaggatcag ccctcagacc aaaatcgtgg
     4981 cggagcttta tcatccggcc ggcaccaagg attacggtcc ttacgcgagc cagctgattg
     5041 cggcgaagcc ggatgtcatt ttcacgccca actggggcaa tgacctcacg ctgcttttga
     5101 aacaggggcg tcccatgggg atgaaacaga aagttttctc ttactatatc aacgatgaag
     5161 tcacgattga atcgctgggc aacgacagtc tgatgatcgg caatatgggc gcggaaatct
     5221 acaccctgtc cattcccacc aaaaagaacg aggagtttgt cgccaaattt tacaaggaca
     5281 aaggctatta cccgacctgg ctgcgcggca aagcttacat tgccaccatg ttctgggcgg
     5341 aggcggttaa aaaggccgga acgaccgacg tggcggcggt catcaaggcg tgggaaggcc
     5401 tgtcctatga cggtccggcc ggcgtctgga ccatgagagc ctgcgatcat caggcgcaag
     5461 tgccttactg gtacacggaa atcgtggcca agaccaaata cttcaaacat gcttttgaag
     5521 ccccccgggg catgaccccg gcgaaagccg tggaagttcc ctgtgcggat acgggctgca
     5581 agatgaagta gcggaaagaa tgtccgagac cggacattcg gatggtgttt caaaataacc
     5641 gttcccctcc ttgtccggat gaccccggac tcccgctgcg ggggtcccgg gtcatccccg
     5701 ggaacggaag ataagttcag cgtcgagtgt tgaggttcag aaagagcgag gacgtcctat
     5761 ggcagaagta agcggcaagg tcatgagcgc caaagaggcc gttgaccgat tcgttcacaa
     5821 cggcgatgag ctggtgatcg gcaattatac cgtaggaagc tgcgccgaac tggtctacga
     5881 gattgccagg cagcgcaaaa aaggcttcac cctgtattcg caatccggca tttttgacgt
     5941 cgaagtcctg gtcgcgggcg gttgcgtcga tcggctggtc acaacctacg tgatgcgttc
     6001 cggcggcaaa aaaggcggtt ccgccgtcga gcgggcgctg aatgccggaa cgctggaaat
     6061 cgaagactat accaattttg aatataacgc ccggctggtg gccggcatgc acggtttttc
     6121 cttcatgccg gtgctggagg gggtcatggt gacggatatc ttccgcagac ggggcttcat
     6181 gggcgaaaat aaatttcgtg tgattcaatg cccctatacg ggaaaagaca aattaaccgt
     6241 tcccgccgcg gcgccggacg tgtgcgtcgt tcacgtgcag cgggccgacg ccgaaggcaa
     6301 cgcgcagtac tggggcgcca tggggagcgt ggcggcggcg gcgctggcca gcaaaagaat
     6361 catcgtctcc tgcgaagaga ttgtcgatca cgagatcatc cagtcgagcc cccacctgac
     6421 gattatcccg gcctaccggg tgaacgcggt cgttgaagcg ccctggggcg ctcaccccac
     6481 ggaggttctg ggatactaca atgtggacac cttcatgtat aatggattgt tttcaactgc
     6541 ggcggcgacc gcagacggac ttgcggcatg gatggaggaa tgggtctacg gctgtccgga
     6601 ccgcgcgcgc tacatcgacc attacatcgc caggttcggt tcggaggcgc tcgatgaact
     6661 gcgggccaaa cccttttatt ccgcgcccgc cgactacggc gcggcctttt cctcgcactg
     6721 ggatcgcgag gggagagaaa gaagcatggg ggtcaccctt ccggaactgg aaaaattatt
     6781 gaaagacaga ggaaaagttt atgggtaagc cttatacgct cgatgaactg ctggccattg
     6841 cggtggcccg ggagattcac gattatgaga acgtggttct gggcgtcggc gtccccatga
     6901 cggcgggcgc cctggccaag tcgcttttgg cgcctcacgc cacgctgatg atggaatcgg
     6961 gcattgtgga catccgtccc ctcttgtctc tgaacaatat cgccgacgca cattccgccc
     7021 gcggattctc ctatgccacg gatctttttt cggccttcac cgcaacatac cgcggttttg
     7081 ttgatgtctg ttttctcggc gtggcgcagg tcgataaata cggaaacctg aatacgaccg
     7141 tcatcggcga ttacaacaaa ccggacatgc gcctgccggg ctccggcggg gccgcggatt
     7201 tcatctccta cgccaggcgg accgtcctca cgatgcgggg cggcgccttt gtcaacaaac
     7261 tggattattt cacctccccg ggctacctcg aaggcggaga cagccgcgac aaaacgggcc
     7321 tgtttccgat cggctccggg ccgactgccc tgatcagcac aaaaggcgtc ttccgttttg
     7381 acgcaacaac caaggagatg tatctcgcca cgattcatcc gggcgtgagc ctcgaatccg
     7441 tcaggaagga catcccctgg aatttgaagg catccccgga tctgtcgctg accgaacgtc
     7501 caacggatga ggagatcgat tttatccgca atttcgcccc ggagctttcc gccggccgtg
     7561 aattgaccgt ggaattgcgc aacgccgccg tcaagaaaaa agccgagctg cgcgccggga
     7621 aaaggcctgg cgcgggagga cgattatgaa actgaaaaat aaagtagccg ttgtgacggg
     7681 cgccagcaag ggcatcggca aggccgttgc cctggcattc gcccgggagg gcgcctccgt
     7741 cgttccggcc agccgaacgc cggccgcgct ggaagatgtc gtccgggaaa tcagggcggc
     7801 aggcggcgag gcctcggccg tttccgtcga cgtgagaagc gttgacagta tcaacgctct
     7861 ggtgaagaag gccgttgacc gttacggccg tctcgatctg ctggtgaaca acgccgggat
     7921 cacgatgggc ggcccttccg aggatctggc gccggaagat tggcgcgcgg ccctggaaac
     7981 ggatttgttc ggtgtgttct acgcctgtca ggcggcggcg cgcgtcatga tcccgcaggg
     8041 cggcggcaac atcatcaata tttcgtccgt caacggaatt ctggcggcgc cgaggcgcgc
     8101 cgcctattgc gccagcaaag cggcggtgaa tgagctgacc aaggttctgg ccattgaatg
     8161 ggccgaccga aagattcgcg tgaacgccat tgcccccgga tatgtccgga cggagctggt
     8221 cgaggacgtc atccataaag gagcgatttc cctggaagcg attctcaagc gcacaccgca
     8281 gggcaggatc ggagaagtcg gggatatcgc ctcgctggcc gtgtatatgg ccggcgacga
     8341 atcctcctat atgaccggaa gcatcgtcaa tattgacggc ggctggctgg cctacgggta
     8401 tttatgaaga acattgcatt ttaagcgatc tatggaggca tttcattatg acggagaaaa
     8461 tagccgttat cggttcaggg acaatggggt ctggcattgc gcaggttgcc gtggaagcgg
     8521 gtttccaggt cagcctgg
//
LOCUS       DAOPAF010000066         8458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_7807_length_8458_cov_44.121266, whole genome shotgun sequence.
ACCESSION   DAOPAF010000066 DAOPAF010000000
VERSION     DAOPAF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_8458_cov_44.121266"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1478
                     /locus_tag="PLQ77_06620"
     CDS             159..1478
                     /locus_tag="PLQ77_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPL10385.1"
                     /translation="MENLSDSKKNDVLPEFQTFLLEKKLVPDKHVFFYALWASKYFTY
                     ARKKQINSDQFQENAVIEFIEFLKADSNISDWQIRQAGDAIRLYYFHFRGLRPDNLST
                     IKAHDAIPELLKETQRLIRLKHYSYSTEKTYLQWIKRFLEYLQQTSKKDAFAEVDAAD
                     CKNFLSHLALNEKVAASTQNQAFNALLFLFRNVLCKEVGGLAQTVRAKRGQKLPVVFS
                     MEEVKRLLACLSGRDLLIAGLIYGAGLRLMELARLRVKDIDMDLNTLTVRSGKGDKDR
                     TTILPATVKNQLNNHLVEVKKMYENDLARGYGEAHLPDALSRKYPNAGKEWAWQYVFP
                     ANHLAVDPRSGKVRRHHISDTAIQDVIKKAIRKAGIPKFATVHTLRHSFATHLLMNGV
                     NIREVQELLGHKNVETTMIYTHVIRDMSHAPQSPLDTLLAGTKNQTG"
     gene            1586..2212
                     /gene="hisH"
                     /locus_tag="PLQ77_06625"
     CDS             1586..2212
                     /gene="hisH"
                     /locus_tag="PLQ77_06625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPL10386.1"
                     /translation="MIAIIEYNAGNITSVARALHSIGQDFVITDDTKMLNAASHAIFP
                     GVGAAGEAMAYLKDKKLDVWLKQCFRSGKPILGICLGTQIILDASEENDAACIGLVGG
                     TTRRFPESLTAAGRALKIPHMGWNSVAFRRPHPVFAGVPEEAEYYFVHSYYPSPESEE
                     AVLGTTEYGMTFCSVLAVRNLIAMQFHPEKSGRPGLQILKNFCAWEGK"
     gene            2209..2988
                     /gene="hisF"
                     /locus_tag="PLQ77_06630"
     CDS             2209..2988
                     /gene="hisF"
                     /locus_tag="PLQ77_06630"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPL10387.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYEQGA
                     DEIVFYDITASSDKRDIMIDVVRRVAETIFIPFSVGGGIRNLDDMRKVIGAGAEKVSV
                     NSAAVLEPRIISQGARAFGSQCVVLGMDVKKADVSGQTPSGYEIVIEGGRKHTGIDAL
                     EWAKEAERLGAGEICLNSIDADGMQDGYEINLTALISENVHIPVIASGGAGKPEHLAD
                     VLTAGRADAALVASMVHYRTYSILQIKKYLSDRHIKTRMLW"
     gene            3110..3991
                     /gene="pdxS"
                     /locus_tag="PLQ77_06635"
     CDS             3110..3991
                     /gene="pdxS"
                     /locus_tag="PLQ77_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPL10388.1"
                     /translation="MDAKRYELNVQLAQMLKGGVIMDVTNVEQAKISEEAGAVAVMAL
                     ERVPADIRKDGGVARMSDPAMIAAIKKAVSIPVMAKARIGHFVEAQILEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQVAQMPVEEVTGFAKTNGLPYELALKVKELGRLPVVNFAAGGIATPA
                     DAALMMQLGCDGVFVGSGIFKSADPARRARAIVKATAYFNDPQKVLEASMDLGEAMPG
                     LEIGQIPPEQILAKRGW"
     gene            4001..4678
                     /gene="pdxT"
                     /locus_tag="PLQ77_06640"
     CDS             4001..4678
                     /gene="pdxT"
                     /locus_tag="PLQ77_06640"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPL10389.1"
                     /translation="MSVRLVGDEPGIIGVLDLQGGVHEHLEHLERLGLPYKRVKQASD
                     FADLAGLIIPGGESTCLARLLTIFDLKDVILQHHSRGMKIWGTCAGAILLATEVTGEK
                     PCLGLIDMEIERNGFGSQLDSFKSEAFIGAVSEEPVPLTFIRAPKILHAGEDVHVLLR
                     MDDYIAAVENEHILVTVFHPELTGSLAFHRYFALKCGLNPAAEKSSAADSGWKNVSWM
                     KLARIAS"
     gene            4683..5462
                     /locus_tag="PLQ77_06645"
     CDS             4683..5462
                     /locus_tag="PLQ77_06645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPL10390.1"
                     /translation="MSYVRIIEDTFFAVCRYWGGLQNAFDVFGPVRAMQTGIASADLN
                     MVWSESPLTQGDAPCVRQVEKYYETESLPYWWWIFPRAKTAETIRLLKDTGHVHLLNM
                     PCLLADLSALPKAKSCGPPATIREVGNQEDLALWQEVTFAGFDFPPGDKISHDRFTGS
                     FSLDPGSSQKAFLAFDGGRPVAASLLFLAGNAAGLYFITTLGEYRRRGIGRKLVLATM
                     HHARAAGARYASLQSSPDGLTVYEQAGFKEFCRVDIYGRKG"
     gene            complement(5520..6101)
                     /locus_tag="PLQ77_06650"
     CDS             complement(5520..6101)
                     /locus_tag="PLQ77_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPL10391.1"
                     /translation="METILSIAVGIGLAAACGFRVFVPLLILNLATLGGALTLPSGFA
                     WMGSHYATVGFSVATAAEVIGYYVPWFDHVLDTIATPAAIAAGTLTTAAFLTDVSPFF
                     KWTAALIAGGGTAGLIQASTVFLRAKSSLATGGAANPLFSTMENVSAVVTAILAILLP
                     LVCIVLMLIFCVWAARKVIRFFLKKNKVGTKSP"
     gene            complement(6177..7604)
                     /gene="purF"
                     /locus_tag="PLQ77_06655"
     CDS             complement(6177..7604)
                     /gene="purF"
                     /locus_tag="PLQ77_06655"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPL10392.1"
                     /translation="MLPFVEGEQNDPGRPREACGLFAIYGNEDAARVTYFGLFALQHR
                     GQESAGVVVSNGCSVFEHKGMGLASTVFNENILQKLTGSLAIGHVRYSTTGSSTLSNA
                     QPFLVHVGDEYYALGHNGNIVNAQSLRSELEGRGSIFQSTMDSEVIVHLMAPHLKRGL
                     EKALCIALQQVEGAYSLVMLTRNRIVAARDPRGFRPLALGKLNGGWVVASETCAFDLV
                     GAEYIRDVEPGEILIIDADGCKSLKPFAPVKPCHCIFELIYFSRPDSQIFGQNVYLCR
                     KRLGHELAREYRPDVDLVMPFPDSGNYAAIGYAEESGIPFEMGMIRNHYVGRTFIQPT
                     QPMRDFGVRVKLNPVKPLLAGRKVMIVEDSVIRGTTSRNRVKNLREIGVKELHMVISC
                     PPTLFPCPYGIDFSSKGELIAAKKETEQAIAEFIGLDSLHYLTVDGMVRATGLSKDCF
                     CLACYTGDYPIAPPVKIDKYCMELN"
     gene            complement(7743..>8458)
                     /gene="carB"
                     /locus_tag="PLQ77_06660"
     CDS             complement(7743..>8458)
                     /gene="carB"
                     /locus_tag="PLQ77_06660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HPL10393.1"
                     /translation="AIGVPLAKLATRVMLGKTLGELSFTKTVIPKHVCVKEAVFPFNR
                     FPGVDILLGPEMKSTGEVMGIDHSFGLAFAKSQMATGFKMPTSGSVFISVHDCHKAGI
                     ADVARSLADLGFNILATAGTAGYLNQRGIIATVVNKVSEGRPHVVDLIKNGDIQMVIN
                     TSVGRKSTQDAYLIRHGALSYNIIYTTTLAGARALGAAARALKEEGWGVCPLQEYYKK
                     QHLPLLPSLDTLKGTSGRN"
BASE COUNT         2010 a         2180 c         2379 g         1889 t
ORIGIN      
        1 aacaattaat ataccaactt ggtttttccg actttcgggt ttaatattac tcttttttaa
       61 gaagaatgca aaaataaatt gctttaaaat ccagctaaat gaactttttc tgcttggtat
      121 tttgtggtaa tatcgcactt aagccaagtt ggtatattat ggaaaatctg tccgacagta
      181 agaaaaatga tgttcttcca gagtttcaga cctttctttt agagaagaaa ttggtgccgg
      241 acaagcatgt cttcttttat gccttgtggg caagtaaata tttcacctat gcccgcaaaa
      301 agcaaataaa ctctgatcaa tttcaggaaa acgcagtcat cgaatttatt gaatttctga
      361 aagcagattc caatatatcc gactggcaaa tccggcaggc aggtgacgca atccgcttat
      421 actattttca ttttcgcggt ttaagacctg ataatttatc aacgataaaa gcccatgacg
      481 ccatacctga actacttaaa gaaacgcaac ggctcatccg tctgaagcat tattcctaca
      541 gcacggaaaa aacttatctg caatggatca aacgttttct cgaatacttg cagcaaacct
      601 cgaaaaagga tgctttcgca gaagttgacg ccgccgattg caaaaatttt ctcagccatc
      661 tggctttaaa tgagaaagtt gccgcatcaa cgcagaatca ggcgttcaat gccctgcttt
      721 ttctctttcg caatgtgctt tgcaaagagg tgggtggcct tgctcaaact gtccgggcaa
      781 agcgcgggca aaaactgccc gttgtctttt caatggaaga ggtcaaacga ttgctggcct
      841 gtttaagcgg cagggatttg ctcattgcag gattaatcta tggcgcgggt ctaaggctga
      901 tggaattggc ccgcctgcga gtgaaagaca tcgatatgga tttaaataca ttgacggtgc
      961 gcagtggcaa gggtgacaaa gacagaacaa ccatcctgcc tgcaacggtc aagaatcagt
     1021 taaataatca tctggtcgaa gtgaaaaaaa tgtatgaaaa tgatctggcc cgaggatatg
     1081 gcgaagccca tcttcccgat gcgctgtcca gaaaatatcc taacgccggc aaggaatggg
     1141 catggcaata tgttttcccg gcgaatcatc tcgcagttga tccgcgctca gggaaagtgc
     1201 gccgccatca catcagcgat acggccattc aagatgtcat aaaaaaagca atccgcaagg
     1261 ccgggattcc caaatttgct accgtccata cccttcgtca tagttttgcg acgcatttat
     1321 tgatgaatgg cgtgaatatc cgtgaagtgc aggaattact cggacacaag aatgtagaaa
     1381 caacgatgat ttatacccat gtcataagag atatgtctca cgcgccgcaa agcccgcttg
     1441 atacattgct ggctgggacg aaaaatcaga cgggatgaac agctttatcg caagcataat
     1501 tcaaattgat cttggcgttt aggtattaaa tttgctagta gcaatgttat gaatgcgtta
     1561 atatttttcg aggacattaa gcagaatgat tgcaattatc gaatataatg ccggaaacat
     1621 taccagtgtg gcgcgtgcgc tgcattctat aggacaggat tttgtcatta cggatgatac
     1681 aaaaatgctc aatgctgcat cccatgctat ttttccgggg gtcggcgcgg cgggcgaggc
     1741 gatggcttac ctcaaggaca aaaagctgga tgtgtggctc aaacagtgtt tccgatccgg
     1801 aaagccgatc ttgggcattt gcctgggcac gcagattatt ctggacgcct ccgaagaaaa
     1861 cgacgcggcg tgcatcggcc tggtcggcgg gaccacccgg cgttttccgg aaagcctgac
     1921 cgcggccggc cgggctctga aaatacccca catgggatgg aacagcgtag ccttccggcg
     1981 tccgcatccg gtgtttgccg gagtgccgga agaggcggaa tattactttg ttcattccta
     2041 ttatccgtcg ccggaaagcg aagaggcggt tttggggacg acggaatacg gcatgacctt
     2101 ctgctcggtg ctggcggtga gaaacctgat tgccatgcag ttccatccgg aaaaaagcgg
     2161 ccggccgggc ctgcaaattc tgaaaaattt ctgcgcgtgg gagggaaaat gatctcgaaa
     2221 agaatcattc cctgcctgga cgtgcgtgac gggaagctca ccaaaggcat taaatttaaa
     2281 ggcaatgttg atatcggcga tccggtggag gcggcgcgag aatactacga gcagggcgcg
     2341 gatgaaattg tcttttatga catcacggct tcatccgata agcgcgatat catgatcgat
     2401 gtcgtgcggc gagtggccga aaccattttt attccctttt ccgtgggagg cggcattcga
     2461 aatctggatg atatgcgcaa ggtcatcgga gccggagcgg aaaaagtcag cgtgaattcg
     2521 gcggcggtgc tcgaaccccg gatcatctct caaggcgcaa gagcgtttgg aagccagtgc
     2581 gttgtgctgg ggatggatgt gaaaaaggcg gacgtttccg gacaaactcc ctccggatac
     2641 gaaatcgtca ttgaaggagg aagaaaacac accggcatcg acgcgcttga gtgggccaaa
     2701 gaggcggaaa gactgggcgc gggagagatc tgcctcaatt ccattgacgc cgacgggatg
     2761 caggatggct acgaaatcaa tttaaccgcg cttatttcgg aaaatgttca tattccggtg
     2821 attgcctccg gcggcgcggg caaacccgag catctggcgg acgttctgac cgcgggcagg
     2881 gccgacgcgg cgctcgtcgc ctccatggtt cactaccgga cttattccat tttgcagatt
     2941 aaaaaatatc tgagcgatcg tcatatcaag acccggatgc tctggtaacc gccaattatt
     3001 atacttgacg aaattccatt tgtattctta atagccaatg attaaattat tttaaatttt
     3061 tgtgaaccca aaagaggata tttttagaaa gtcaggaggc gaataaaaag tggatgcaaa
     3121 acgatatgaa ttaaatgtcc agttggcgca gatgctgaaa ggcggcgtca ttatggatgt
     3181 caccaatgtg gagcaggcca aaatttcgga agaggcgggc gccgtcgctg tgatggcgct
     3241 ggagcgcgta cccgcggata tccgcaagga cggcggcgtg gcccgcatgt ccgatccggc
     3301 gatgatcgcc gcgatcaaga aggccgtgtc gattccggtc atggccaagg ccagaatcgg
     3361 tcattttgtg gaagcgcaga ttctggaagc cctgcggatt gactacattg atgaaagcga
     3421 agtcctgacg ccggccgacg aggaatgcca tattgacaag acaaaattct cgataccgtt
     3481 tgtctgcggc gcccgcaacc tgggtgaagc cctgcgccgg atcgcggagg gcgcggccat
     3541 gatccgcacc aagggcgagg ccggaacagg caacgttgtg gaagccgtcc gccacatgag
     3601 aaccatgaac cgggaaatcc gccaggtggc gcaaatgccg gtagaggaag tcaccggatt
     3661 tgccaaaacc aacggcctgc cgtatgagct ggcgctgaaa gtgaaagaac tgggacgtct
     3721 gccggtggtc aattttgccg cgggcggcat cgcgacaccc gccgatgcgg cgctgatgat
     3781 gcagctcggt tgcgacggcg tttttgtggg ctccggcatc ttcaaatccg ccgatccggc
     3841 ccgacgcgcc cgcgccatcg tcaaggcgac cgcctatttc aacgatccgc aaaaagtcct
     3901 ggaagcgtcg atggatctgg gcgaggccat gccgggcctg gaaatcgggc agattccgcc
     3961 ggagcagatt ctggccaagc gaggctggta agtatttgtt atgtcagtgc gtttagtggg
     4021 cgatgaaccg ggaatcatcg gtgttctgga tttgcagggc ggtgttcacg agcacctgga
     4081 acatctggaa agattgggac ttccctataa gcgagtcaaa caggcgtcgg attttgccga
     4141 tcttgcagga ttgattattc ccggcggcga aagcacctgt ctggcgcggc ttctgaccat
     4201 ctttgacctg aaagacgtta ttctccagca tcacagccgc ggcatgaaga tctggggcac
     4261 atgcgccggc gccattctgc tggcgacgga agtgaccggc gaaaaaccat gcctgggcct
     4321 gatcgacatg gaaatcgaac gcaacggctt cggcagccag cttgacagct ttaaaagtga
     4381 agcgtttatt ggagctgtat ccgaggagcc ggttccttta accttcatcc gcgcgccgaa
     4441 aattctccac gcgggagaag atgttcacgt tctgctccga atggacgatt atatcgccgc
     4501 agtggaaaat gagcatattt tagttacggt ttttcatccc gagttaaccg gctcgctggc
     4561 ctttcaccgg tattttgctt tgaaatgcgg cctgaacccc gccgccgaaa aatcttccgc
     4621 cgccgattcc ggctggaaaa acgtaagctg gatgaaactg gcgcgcattg cctcgtaagc
     4681 ccatgtctta tgtccgcatc attgaagata ctttttttgc cgtctgccgc tattggggcg
     4741 gccttcaaaa cgcctttgac gtttttggtc cggtccgggc gatgcagacg gggatcgcct
     4801 cggccgatct gaacatggtc tggagcgaat cgcccctgac acagggagac gcgccatgcg
     4861 tccggcaggt tgaaaaatat tatgaaacgg agtccctgcc ctactggtgg tggatatttc
     4921 cccgcgccaa aaccgcggaa acgatccggt tgctcaaaga cacaggccac gtgcatctgc
     4981 tgaacatgcc ttgcctgctg gcggatctca gcgcattgcc gaaggcaaaa tcctgcgggc
     5041 cgcccgcaac gatccgcgaa gtcggcaatc aggaagatct ggcgctgtgg caagaggtga
     5101 cctttgccgg ttttgatttt ccacccggcg acaagatttc gcatgatcgc tttaccggat
     5161 ccttttctct cgaccccggc tcttcacaaa aagcctttct ggcgtttgac ggcggcagac
     5221 ccgtggctgc ctccctgctt tttctggcag gtaatgccgc gggcctctac ttcatcacga
     5281 cgcttggcga gtatcgccgt cggggcattg gtcgaaagct ggtcctggcg acgatgcatc
     5341 atgcccgggc ggcgggggcc cgttatgcat cgcttcaatc ctcgccggat ggtttgaccg
     5401 tctatgagca ggccggattt aaagagttct gccgcgtgga catttacggc cggaaggggt
     5461 gagaggcaaa agcgtttgtt tcaggcgctg cgttccgtgg cgcccgtccc aagtttacat
     5521 caggggcttt tcgtcccgac tttgtttttt ttcagaaaaa atcggatcac ttttcttgcg
     5581 gcccacacgc agaagatcag cataagcaca atacagacaa gaggcagcag aatggccagg
     5641 atcgccgtga ccaccgcgct cacattttcc atcgtggaga agaggggatt ggctgccccg
     5701 ccggttgcca gggaagattt tgcacgcaaa aagactgtcg aagcctgaat caggcctgcc
     5761 gtgccgccgc ccgcaatcag agccgctgtc catttgaaaa acggtgaaac gtccgtcagg
     5821 aaggcggccg tggtcagggt tcccgccgca atcgccgcgg gtgtcgcaat cgtgtccagc
     5881 acatgatcga accagggaac atagtagccg atgacttcgg ccgcggtcgc gacggaaaaa
     5941 ccaaccgtcg cgtaatggct tcccatccag gcaaagccgg aaggcagggt cagcgctccg
     6001 ccgagcgtcg ccagattgag gataagcaga gggacgaaga ctcgaaaacc gcaggccgcg
     6061 gccagcccga ttcccaccgc gatggagaga atagtttcca tgcctggctt tttacacccc
     6121 gtggcggaaa aagggaagcc gaattcggga gagcttgaag cctcccgaag gaaaaatcaa
     6181 ttcaattcca tgcagtactt gtcgattttt acgggcgggg cgatggggta gtcgccggtg
     6241 tagcaggcca ggcagaagca gtctttggac aggccggtgg cccggaccat gccgtccacg
     6301 gtcagatagt gcagcgaatc caggccgatg aattcagcaa tcgcctgttc ggtttccttt
     6361 ttggcggcga tcagttcccc cttggaggaa aaatcaatcc cgtagggaca ggggaaaagc
     6421 gtcggcggac aactgatgac catgtgcagt tccttcacgc cgatttcccg caggtttttc
     6481 acgcgattgc ggctcgtggt gccgcgaatg acggaatcct cgacgatcat gacttttcgg
     6541 ccggccagaa gcggcttgac tggattgagt ttgacgcgca cgccaaaatc gcgcatgggc
     6601 tgcgtcggct gaatgaacgt gcgccccacg taatgattgc ggatcattcc catttcaaag
     6661 ggaatgccgc tttcctccgc gtagccgatg gcggcgtagt ttccggaatc cggaaaaggc
     6721 atgaccagat cgacgtccgg ccggtattcc ctggccagtt cgtggcccag ccgcttgcgg
     6781 cagagataca cgttctggcc gaagatctgg ctgtcgggac gggaaaaata aatcaactcg
     6841 aaaatgcagt ggcagggctt caccggcgcg aagggtttca ggctcttgca cccgtcggca
     6901 tcaatgatca ggatttcccc cggttcaacg tcgcggatat attcagcgcc caccagatcg
     6961 aaggcgcagg tttcggaagc caccacccag ccgccgttca gttttcccag cgccagaggg
     7021 cgaaaaccgc gcgggtcgcg ggcggcgacg atccggtttc gggtcagcat caccagagaa
     7081 taggcgcctt caacctgctg aagcgcgatg caaagcgcct tttccaatcc ccttttcaga
     7141 tgcggcgcca tcaggtgaac aatgacttca ctgtccatcg tggattggaa aatggagccc
     7201 cggccttcca gttcggaccg cagggactgg gcgttcacga tgttgccgtt gtgccccagc
     7261 gcgtagtatt cgtcgcccac atgcaccaga aacggctgcg cgttggaaag ggtggaggaa
     7321 ccggtcgtgg agtagcgaac atggccgatg gccagagagc cggtcagttt ttgcagaata
     7381 ttctcattga agacggtgga ggcaaggccc attcccttgt gttcgaaaac gctgcatccg
     7441 ttggagacga cgacgccggc ggattcctgg ccgcgatgct gcagggcaaa aaggccgaag
     7501 tacgttacgc gggcggcatc ctcattgccg taaatggcga aaaggccgca cgcctcgcgg
     7561 ggccggccgg gatcattttg ttcgccttca acaaagggaa gcattttgtt tctccatatt
     7621 ttccctcccc ttgtgggagg ggatgaaggg gagggggcgt ttcaccctcc ctcaatccct
     7681 cccatcaagg gagggaagaa aaggttctga ttgcgtccaa acattcaacc tcttggtgaa
     7741 cctcaattcc tcccacttgt gcccttcagg gtatcaaggg agggaagcaa aggaaggtgt
     7801 tgctttttgt aatactcctg aagggggcag accccccagc cctcttcttt taacgccctg
     7861 gccgctgcgc cgagggcccg cgcgccggcc agtgtcgtgg tgtaaatgat attgtacgat
     7921 aaagcgccgt gacggatcaa atacgcgtct tgcgtggact tgcgtccaac gctggtgttg
     7981 atcaccattt ggatgtcgcc gtttttgatc aaatccacca catgcggacg cccctcactc
     8041 accttgttga ccacggttgc gatgatgccc cgctggttca ggtatccggc cgttcccgcc
     8101 gtcgctaaaa tgttgaaacc cagatcggcc agtgaccggg ctacgtccgc gatccccgct
     8161 ttgtggcaat cgtgaacgct gatgaagacg ctgccggacg tcggcatttt aaagccggtg
     8221 gccatctgtg acttggcaaa ggccagaccg aaggaatgat cgatgcccat cacctcgccg
     8281 gtggatttca tctccggccc caggagtatg tccacgccgg gaaagcgatt gaacggaaag
     8341 accgcctctt tcacacagac gtgtttggga atgacggttt ttgtaaaaga cagctcgcct
     8401 aaggttttgc ccagcatgac ccgggtggca agcttcgcca gcggcacgcc gatggcct
//
LOCUS       DAOPAF010000067         8410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_7853_length_8410_cov_41.908917, whole genome shotgun sequence.
ACCESSION   DAOPAF010000067 DAOPAF010000000
VERSION     DAOPAF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_8410_cov_41.908917"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLQ77_06665"
     CDS             complement(<1..228)
                     /locus_tag="PLQ77_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPL10394.1"
                     /translation="MKEISVRSSSRFEMIDITEAVRKTAREEKIESGIVLVYTPHTTA
                     AVTINENADPDVPRDLLAALDRAVPLSANYRH"
     gene            complement(244..912)
                     /locus_tag="PLQ77_06670"
     CDS             complement(244..912)
                     /locus_tag="PLQ77_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPL10395.1"
                     /translation="MEKSLQRQARFHKPSSVMPLFVIIALLLCAAGCAGGGKPQYDVQ
                     KYLLAYPSPTWDGSAKLPVSVKVNRFSIAAAYNSTGMIFRDDAYGLDAFNYSRWAVNP
                     ADMIGDLLLADMRAGNLFQAVLSRYDLEEGRFILSGGIEEFYLQTEKKNRAARIVIAI
                     SLQDSREKETGKKMMYQKTYTREAPLQEASPKGYCAAASQAMQALSREIIGDVYSAVR
                     ARTP"
     gene            complement(928..1860)
                     /locus_tag="PLQ77_06675"
     CDS             complement(928..1860)
                     /locus_tag="PLQ77_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPL10396.1"
                     /translation="MPTRSSKFSIGLFVIVGALICAGVIIWVGAAKFFTKGSFYNVYF
                     DESVQGLQVDSAIKYRGVEIGKVQKIDVAPDYRLIEVTMKIDLEGDLQKQTIATLKTA
                     GITGIVFIELDRIKPGELADSPKLTFKADYPVIPSRRSEISRLIGDTNMIMQNIKKID
                     FEGISSQLKNTAQAIENFVEGKRITSLMTHLENTSANLDETIAGINKTVAEGKVDRAV
                     TEALGVLTDARKLVGRAREEIGALNLREKSAQTDAILQNIDKKTKIITTGLADTSENL
                     RVTSENLQRLAESLNRDPSDLIYTKPAPPRKPME"
     gene            complement(1850..2647)
                     /locus_tag="PLQ77_06680"
     CDS             complement(1850..2647)
                     /locus_tag="PLQ77_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL10397.1"
                     /translation="MTEPSEKKTVLSVKNLTQRFDDNIVFEDVSFDVYQGEVLVIVGG
                     SGCGKSTLLKIMIGLQKPYAGQVLYQGTDITRASDEALNEYRKNIGVLFQSGALFSSM
                     SIKENIALPLQEYTDLDADTIDRVIRMKLGMVNLAGYENHNPSELSGGMKKRAGIARA
                     MALDPRVLFLDELSAGLDPITAVELDDLIIKTNEALGTTMVIVTHELESIYKIAHRVL
                     MLDKEARGMIAEGRPLELKENATDPRVRSFFLRQLPENQDEQRFYAD"
     gene            complement(2667..3836)
                     /locus_tag="PLQ77_06685"
     CDS             complement(2667..3836)
                     /locus_tag="PLQ77_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HPL10398.1"
                     /translation="MTTPRNIDGQDGYQVSFQKENESLLISLKGEFGLKNLTAFTREI
                     RQAVSGHAIPTLTIDFSQVKYLDSAAALAFVQMEQEAASRKTNCHLANLSEEARGIFS
                     VIHPDVLTRRPFKPEHTGEGVVQQIGQASLSIGRDFVNFITFIGDLLSALAYTVTHPA
                     TLRGKDVLFYVQRAGVDGLPIVILIGALMGLIMAFMSYLQFKQFGANIYVPTLVSFAM
                     IKELGPIMTAILVAGRSGSAFAAEIGTMQVNEEVDALETMGFDPVRFLAVPKVLAAVI
                     VVPILTVYADVSGILGGLIIGVTTLDLTFKTYISQSIKTIQVFDVVTSLIKAAVFAGL
                     ISAIGCQRGFQVRAGAQDVGKFTTSAVVAAIFLIVLTDSIFAVMLYYVKRYSAFA"
     gene            complement(3994..4197)
                     /locus_tag="PLQ77_06690"
     CDS             complement(3994..4197)
                     /locus_tag="PLQ77_06690"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPL10399.1"
                     /translation="MNCKKERNLKICGCTYEPCSRKGICCDCVAYHLQKRQVPGCFFP
                     QDAERTYDRSFENFADLVAKKRI"
     gene            complement(4418..5104)
                     /locus_tag="PLQ77_06695"
     CDS             complement(4418..5104)
                     /locus_tag="PLQ77_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL10400.1"
                     /translation="MKNEKAAKIIDKALKQGRKTLSEYEAKQVLAAYGIPVTKEVLAR
                     DKTALEKGLKKIGFPLVMKGCSADIAHKTEKGLIHVDIRTATEAKKAFREILAGMEGY
                     EGGVLMQEMIKGRRELVMGLTRDPQFGPCVMFGLGGIFTEILRDVAFRRAPLEIRDAK
                     EMMQEIKGRKILDAVRGMEAADQKMLVDMLISVGRIGLDFDDIMEIDLNPVILSGSKP
                     VVVDALIVLK"
     gene            complement(5101..6603)
                     /locus_tag="PLQ77_06700"
     CDS             complement(5101..6603)
                     /locus_tag="PLQ77_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL10401.1"
                     /translation="MSSKTRRTGKTGKAHYPPTDYERIFHPKAIAILGVSADENGVGF
                     GTGMLRAIMAMGYEGKLYPVNPKGGTIYGLEIHRMVEDIPGPVDFAVVAVNARYVPES
                     LEACLKIGVAGAEILSSGFSELGTEEGKELERRIQDIAARGIRVVGPNCFGIYCPASG
                     LTLLPGPDLSRETGPVAFVAQSGGMSIDFAHAGKWLGVRFSKMISFGNGADLREAELL
                     RYLADDAETRVISMYIEGIKDGERFFSELKAAAAKKPVIVYKGGLSVAGARSVASHTA
                     SMGGSRVIWQSILKQAGAIQVNDTEEMAQANLAFAMLPPKPFRGISIVGGGGAIGVTA
                     CDTAETFGIEIPALPPQLQERIGSVLPKPGSSAINPIDVANPFVGPQALKEVLLGAAE
                     DERIELQIIVSLLFHYKGLARAFGRPVAEVTPYRELAQCVRDAIDRTGKPVIAVLPNM
                     TRGVESMDITELIALTRQEYLSRGIPVFDELHDAIRAISHINNYYGGKIK"
     gene            complement(6658..7068)
                     /locus_tag="PLQ77_06705"
     CDS             complement(6658..7068)
                     /locus_tag="PLQ77_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPL10402.1"
                     /translation="MISYQDVILFILSKANQKVYGTFKSRTQLYGLTPIQGLVLHALY
                     EEEGLSAGELGKRLSLDSATLSGVLDRMAESGWIIKNVKDDRRVLNIRLTDKALECRD
                     KFINDTEDLNREIFSRYTLEERLLLIRMLKDLRR"
     gene            complement(7235..7750)
                     /locus_tag="PLQ77_06710"
     CDS             complement(7235..7750)
                     /locus_tag="PLQ77_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10403.1"
                     /translation="MKNTKPIKVKNCFIREAGNPKGEGGCARIVSFFLALLVLFLPAA
                     PAAAQWIEYLRDEDGTVFSYHKKLNIDKKNEKHIVQVLGKENISDAGREKYLQSLKQK
                     GLPAAAYKQLSRKMFLCEIDCREKRIRKASLTAYDTNGKVIFSGENPSPEWEPILSDS
                     TGEALQKSVCK"
     gene            7978..>8410
                     /locus_tag="PLQ77_06715"
     CDS             7978..>8410
                     /locus_tag="PLQ77_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL10404.1"
                     /translation="MPEAKKTGGFYGWVMMPLLCLVYSIPIGFALYGPPVIYRFMQKD
                     LSWQRGEINLGYTIIGMMLGLGAFIIPWLMDRFGPRKTLAIGASLTAGSVLLMAAAGQ
                     SYPVYLALCVCTGLGISFGSVVPVQALVLLWFNVHRALVMGL"
BASE COUNT         1667 a         2344 c         2357 g         2042 t
ORIGIN      
        1 gtgccggtaa ttcgcggaca ggggaacggc ccggtccagc gccgccagaa gatcgcgggg
       61 cacgtccggg tccgcgtttt cattgatcgt gaccgctgcg gtggtgtggg gcgtatagac
      121 caggacgatg cccgattcga tcttttcctc gcgcgccgtc ttccgaacgg cttcggtgat
      181 gtcgatcatt tcaaaacggg atgagctccg gacggaaatt tccttcatga aacacctgtt
      241 cctttacggt gttctggccc tgaccgccga atagacgtcg ccgatgatct ctcgggacag
      301 ggcctgcatg gcctggcttg cggcggcgca gtatcctttg ggcgaggctt cctgcagggg
      361 cgcttcacgg gtgtaggttt tctgatacat catttttttg ccggtttctt tttcccggga
      421 gtcctgaagc gaaatggcga tgacgatgcg ggcggctctg tttttctttt ccgtttgcag
      481 ataaaattct tcgatgccgc ccgagaggat aaaccgcccc tcttccaggt cgtaccgcga
      541 gaggaccgcc tgaaaaagat ttccggctct catgtcggcc aaaaggagat cgccgatcat
      601 gtcggccggg ttgaccgccc agcgggagta gttgaatgcg tccagaccgt aggcgtcgtc
      661 tcggaaaatc atgccggtcg aattatacgc ggcggcaatg gaaaaccggt tcaccttgac
      721 cgacacggga agttttgcag agccatccca ggtgggcgac ggataggcga gcaggtattt
      781 ttgaacgtcg tattgcggct tgccgccgcc tgcgcacccg gctgcgcata acaggagtgc
      841 aatgatgacg aagagcggca tgacggatga tggtttgtgg aagcgggcct gcctttgtag
      901 ggatttttcc attgctcgtt cctttcttta ttccatgggc tttcgcggcg gcgccggttt
      961 ggtgtaaatc aggtccgacg gatcccggtt caggctttcg gcaagcctct ggaggttttc
     1021 cgaggtgacc cgcaggtttt ccgacgtgtc ggcaaggccg gtcgtaatga tcttggtctt
     1081 tttgtcgatg ttctgaagaa tggcgtcggt ttgagccgac ttttcgcgca ggttcagcgc
     1141 tccgatttcc tccctggccc ggccgaccag tttgcgggcg tcggtcagca cgcccagggc
     1201 ttccgtgacg gcccggtcca ccttgccttc ggcgacggtc ttgttgattc cggcgattgt
     1261 ctcgtccaga tttgccgagg tgttttccag atgagtcatc aggctcgtga tgcgcttgcc
     1321 ctccacgaag ttttcgatcg cctgcgcggt gtttttgagc tggctggaaa ttccctcgaa
     1381 atctattttt ttaatgtttt gcatgatcat gttcgtgtcg ccgatcagcc ggctgatttc
     1441 cgaacggcgc gacgggatga ccgggtagtc cgccttgaag gtgagctttg gagaatcggc
     1501 cagttcgccc ggtttgatcc ggtccagctc gatgaagaca atgccggtga tgccggcggt
     1561 cttcagcgtc gcgatcgtct gcttctgcag atcgccttcc aggtcgattt tcatggtcac
     1621 ttcgatcagg cggtagtcgg gcgccacgtc gatcttctgc actttcccga tttcgacgcc
     1681 gcggtattta atcgccgagt ccacctgcaa tccctggacg gactcgtcaa agtaaacgtt
     1741 gtagaaagag cccttggtga aaaatttggc ggcgccgacc cagatgatca cgccggcgca
     1801 gatcagcgcg cccacaatga caaaaaggcc gattgaaaat tttgagcttc tagtcggcat
     1861 aaaacctctg ttcgtcctga ttttcgggca actgtctcaa aaagaagctt ctgacccgcg
     1921 ggtccgtcgc gttctctttt aattccagag gtctgccttc cgcgatcatt cccctggcct
     1981 ccttgtccaa cattagcacc cgatgggcta ttttgtaaat agattccagc tcatgggtga
     2041 caatgaccat ggtggttccc agagcctcgt ttgttttgat gatcaggtcg tcaagctcca
     2101 ccgccgtgat cggatcgagc ccggcggaca gttcgtccag aaaaagcaca cgcggatcga
     2161 gggccattgc gcgggcgatg cccgcccttt ttttcatgcc gccggaaagc tcggacgggt
     2221 tgtgattttc atagccggcc aggttgacca tgcccagttt cattcgaatg acgcggtcga
     2281 tggtgtccgc gtcgaggtcg gtgtactcct gcaggggcag cgcgatgttt tccttgatgg
     2341 acatggagct gaacagcgcc ccggactgaa agagaacgcc gatgtttttc cggtattcgt
     2401 tcagggcttc atccgacgcc cgggtaatgt cggttccctg atacaggacc tgtcccgcat
     2461 atggcttttg cagaccgatc atgattttga gcagcgttga tttgccgcag ccgctgccgc
     2521 ccacgatgac cagaacctct ccctgataca catcgaagct cacgtcttca aagacgatgt
     2581 tgtcgtcgaa gcgctgcgtc aggtttttta ccgaaagaac ggtctttttt tccgatggtt
     2641 ccgtcatgga aatcccggtt ccttccttat gcgaaagcac tgtatcgttt gacatagtag
     2701 agcataacgg caaaaatgga gtcggtcagc acgatcagga agatggccgc gaccacggcg
     2761 gaggtcgtaa acttgccgac atcctgggcg ccggcgcgca cctgaaagcc gcgctggcag
     2821 ccgatggcgg aaatgagtcc ggcaaatacc gcggccttga tcaggctggt gaccacatca
     2881 aacacctgaa tggttttaat cgactgcgag atataggttt tgaaagtcag gtccagggtc
     2941 gtgacgccga tgatcaggcc gcccagaatg ccggagacat ccgcgtaaac ggtcagaatc
     3001 ggcacgacga tcacggcggc cagaaccttc ggcacggcca gaaaacggac cgggtcaaat
     3061 cccatcgttt caagcgcgtc cacctcttca ttgacctgca tggtgccgat ttccgcggca
     3121 aaggctgagc cggaccgtcc cgcgaccagg atcgccgtca tgatcggccc cagttccttg
     3181 atcatggcga agctgaccag cgtgggaaca taaatattcg cgccgaactg cttgaactgc
     3241 agataagaca tgaaggccat gatcagaccc atcagcgcgc cgatcaggat gacaatcggc
     3301 agaccgtcca cacccgcgcg ctgcacgtaa aaaagaacat cctttccccg aagggtcgcg
     3361 ggatgcgtca cggtataggc cagcgcggag agcaagtcgc cgataaaggt tatgaaattc
     3421 acaaaatccc ggccgatcga caaagaggcc tgtccgatct gctgcaccac tccctcgccg
     3481 gtgtgttccg gcttgaaagg gcgccgggtc agaacatcgg ggtggatcac gctgaaaatg
     3541 cccctggctt cttcgctcag gttggccaga tggcagtttg tcttccggct tgccgcttcc
     3601 tgctccatct gaacaaaagc cagagccgcc gcgctgtcca ggtatttgac ctgtgaaaaa
     3661 tcgatggtca gggttggaat cgcatgtccg gaaacagcct gccggatttc gcgggtgaag
     3721 gccgtgagat ttttcaggcc gaactcgcct ttcaggctga tgagaaggct ttcgttctcc
     3781 ttttgaaaag aaacctgata accgtcctga ccgtcaatgt ttcgcggagt cgtcatggca
     3841 atgtttttcc cgccccgaat tcgatattgg aaatgacgtg tttgcaggcg tatttaagcc
     3901 gtttgaccgg tgcttgtcaa tgaaaatcaa tgtcgttgcg aagggatcga cgtttgcgcg
     3961 aaggcttgtc cgcggcggat gcgcgccgcc ggatcatatg cgtttttttg cgaccaggtc
     4021 cgcaaaattc tcaaaggagc ggtcataggt tctttcggcg tcctgcggaa aaaagcagcc
     4081 cggcacctgt cttttctgca ggtgataggc tacgcagtcg cagcagattc ccttgcggct
     4141 gcagggttcg taggtgcatc cgcagatttt caggttcctt tcttttttgc agttcataac
     4201 gttcattatg gttcgacagg ctcaccatga caacgtaggg caggccgctt gtggccttta
     4261 ggttattagg cccgccaaaa ccaagttcgc caaaaccgga atgtcaatca acgtgcggcg
     4321 cacctaaagg tgcgccctac ataaagcaaa aataaagagg cgctgtctct atttaatttg
     4381 ttcttccgcc ttcagccttc ggtctttagc cttcagtcta tttcaaaacg atcagcgcgt
     4441 ccacgaccac cggctttgat ccggaaagaa tcaccgggtt gaggtcgatt tccatgatgt
     4501 cgtcaaagtc caggccgatg cggccgacgc tgatgagcat atcgacaagc attttctggt
     4561 ccgccgcttc catgccgcgc accgcgtcga gaatcttgcg gcctttgatt tcctgcatca
     4621 tctctttcgc gtcccggatt tccagaggcg cgcggcggaa agcgacgtcg cggagaattt
     4681 ccgtaaaaat gccgcccagg ccgaacatca cgcagggtcc gaactgcgga tcgcgggtca
     4741 ggcccatgac cagttcacgc ctgcctttga tcatttcctg catcagcacg ccgccttcat
     4801 agccttccat gccggcgaga atttcccgga acgctttctt tgcctccgtt gccgtgcgga
     4861 tgtccacatg aatcaggcct ttttccgttt tgtgggcgat gtcggccgag cagcccttca
     4921 tgaccagcgg gaagccgatt tttttcaggc ctttctccag cgcggttttg tccctggcca
     4981 gaacctcttt cgtgaccgga atgccgtacg cggccagaac ctgcttggct tcgtattccg
     5041 aaagcgtttt gcgtccctgc ttcagggcct tgtcgatgat cttcgcggct ttttcgtttt
     5101 tcattttatt tttcctccat aataattgtt gatgtgggaa atcgcccgaa tggcgtcgtg
     5161 cagctcgtcg aaaaccggaa tgccgcggga aaggtattcc tgccgcgtca gggcgatcag
     5221 ctccgtaata tccatgcttt ccacgccacg ggtcatgttg ggcagcacgg caatcaccgg
     5281 tttgccggtc cggtcaatcg cgtcgcggac gcactgggcc agctcccggt atggggtgac
     5341 ttccgcgacg ggcctgccga aggcgcgggc aagacccttg tagtgaaaca ggagcgatac
     5401 gatgatttgc agttcgatcc gctcatcttc ggccgcgccg agaagcactt ccttcagcgc
     5461 ctgcggtccg acaaaaggat tggcgacgtc aatcggattg atggcgctcg agccgggctt
     5521 gggcaaaacg cttccgatgc gctcctggag ctgcggcggc agcgccggaa tctcgatgcc
     5581 gaacgtttcg gccgtgtcgc aggcggtcac gccgattgcg cctccgccgc ctacgatgga
     5641 aatcccccga aagggtttcg gcggcagcat cgcaaaggcc aggttggcct gcgccatctc
     5701 ttccgtatcg tttacctgaa tggctcccgc ctgctttaag attgactgcc agatgacgcg
     5761 gcttccgccc atcgacgcgg tgtggctggc cacgctgcgg gcgccggcca cggaaaggcc
     5821 gcctttgtaa acgatgaccg gttttttggc cgcggcggct ttaagctcgg agaaaaaacg
     5881 ctctccgtcc ttgatgcctt caatgtacat cgagatgacc cgggtttccg catcatcggc
     5941 cagatagcgc agaagctccg cttcgcgcaa atccgcgccg ttgccgaagc tgatcatttt
     6001 gctgaagcgg acccccagcc attttcccgc gtgcgcgaag tcgatcgaca tgccgccgct
     6061 ttgcgcgaca aatgccaccg gcccggtttc acgcgacagg tccggccccg gcaaaagcgt
     6121 gaggccgctg gccggacagt agatgccgaa gcagttgggc cccaccacgc gaatgccccg
     6181 tgcggcgata tcctgaatcc gccgctccag ttcttttccc tcttcggtgc ccagttcgct
     6241 gaagccggag gagagaattt ccgcgccggc cacgccgatc ttcaggcacg cttccaggga
     6301 ttccggaaca taccgcgcgt tcaccgcgac aacggcaaaa tcaaccgggc cgggaatatc
     6361 ctccaccatc ctgtgaattt ccagcccgta tatcgttcct cccttgggat tgacgggata
     6421 tagtttccct tcatagccca tcgccatgat ggcgcgaagc atgccggttc cgaagccgac
     6481 gccgttttca tccgcggaga cgcccagaat ggcaatcgcc ttcggatgaa aaatcctttc
     6541 gtagtcggtc ggcgggtaat gtgcttttcc ggtttttcct gttcttctgg ttttgctgga
     6601 cattctgcct ccatgcttgc cgattttacc ttgtatgcta aacagcaaca gggatcgtca
     6661 cctgcgcagg tccttgagca tccggatcaa aagcagtctt tcctccaggg tgtatctgga
     6721 gaaaatttcc cggttcaaat cttcggtatc attgataaat ttgtcacggc actccagcgc
     6781 cttatccgtc agccggatgt tcaaaacccg ccggtcgtct ttcacgttct tgatgatcca
     6841 gccgctttcc gccattcggt ccagaacgcc ggacagcgtg gcgctgtcca gggacaatcg
     6901 ctttcccagt tcaccggcgg aaagcccttc ttcctcgtaa agagcgtgca ggacgagtcc
     6961 ctgaatgggt gtaaggccgt acaactgcgt gcgggacttg aaagttccgt acactttctg
     7021 attggccttg cttaagataa acagaatcac gtcctggtaa ctgatcatcg gtgacacccg
     7081 ggtcattatt tagtgtgcaa gataaataaa gggagaggtc aagtcaagaa aaatgttgac
     7141 cggcgttgac cggtaaaaag ttgattggcc gaagaaatcc ccgtacttcc cgcccccaga
     7201 gttttgggcc tgttgattcc ggggtgaagg gaattcattt gcagacggat ttttggagtg
     7261 cttctcccgt tgaatcggaa aggatgggct cccattccgg ggagggattt tccccggaaa
     7321 agatcacttt gccgttcgtg tcataggcgg tcaacgaagc ttttcgaatc cgtttttccc
     7381 ggcagtcgat ttcgcacaag aacatttttc tggacagctg cttgtatgcc gccgccggca
     7441 atcccttttg cttcagggat tgaagatatt tttccctgcc tgcatcggaa atgttttctt
     7501 ttcccagaac ctggacgatg tgtttttcgt tctttttgtc aatgttgagc tttttgtgat
     7561 aggaaaaaac agttccgtct tcatcccgaa ggtattcgat ccattgcgcg gccgcgggcg
     7621 ccgccggcag aaacagcacc agaagcgcca ggaagaaaga gacgatcctt gcgcatcctc
     7681 cttcgccttt cgggtttccg gcttcccgga tgaaacagtt cttgactttg atcggctttg
     7741 tgtttttcat agcgtttcct gtatacggaa aagcacatca ggaatcaaca ggaaatcttg
     7801 catcgaattt tcatggctct ttcgctcggg tttaggcaaa gggctgttct ttaaaaagat
     7861 ggccaacgca aaaagagtcg gttacgctga aacaaacgaa tccggagcaa ggaatggtca
     7921 aatcggggcg taaagaaagc gaaaacgagg ctgccgaatg ttcgggaggg cctcgcaatg
     7981 cctgaagcga aaaaaaccgg cggtttctac ggttgggtga tgatgccgct tctgtgcctc
     8041 gtctattcga tcccgatcgg ctttgcgctc tatgggccgc ccgtcattta ccggttcatg
     8101 cagaaggacc tgtcctggca gcgcggagag atcaacctcg gctatacgat tatcggaatg
     8161 atgcttggtc tgggcgcgtt catcattccg tggctgatgg accgcttcgg cccccgcaaa
     8221 acgctggcca tcggcgcgtc tctgacggcg ggatcggttc tgctgatggc ggctgcgggg
     8281 caatcctatc ccgtttacct ggcgctttgc gtctgcaccg gcctggggat ttccttcggg
     8341 tcggtcgtgc ccgttcaggc gctggttctg ctgtggttta atgttcaccg ggcgctggtc
     8401 atgggcctgg
//
LOCUS       DAOPAF010000068         8261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_8005_length_8261_cov_45.895686, whole genome shotgun sequence.
ACCESSION   DAOPAF010000068 DAOPAF010000000
VERSION     DAOPAF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8005_length_8261_cov_45.895686"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3072
                     /gene="rpoB"
                     /locus_tag="PLQ77_06720"
     CDS             <1..3072
                     /gene="rpoB"
                     /locus_tag="PLQ77_06720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPL10405.1"
                     /translation="EDLDNASIRDTLLMDRIDSEEEAIMEIYRRLRPSNPPTPETATK
                     FFKSLFFELETYDLSDVGRAKMNYKLRLNVPTDLTVLRNEDILASVKYLIDLKNGVGE
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHSTHY
                     GRICPIETPEGPNIGLIVSLSTYARVNEFGFIESPYRLVENGRVLDEVKYMTAIEEEN
                     QLISPADRPLDKKGKFEEELISVRKGGDFVSATPEEIRMMDVAPDQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMCPEVPVVGTGMESVVARDSGAVMVAKRSGVVESVDA
                     SRIVVRADKPEEDGLDTGVDIYTMSKYQRSNQDTCFNQKPIISIGDRVRKRDILADGP
                     ATDNGELALGRNVMVAFMSWGGYNYEDSILVSERIVKEDIYTSIHIEEFETMARDTKL
                     GKEEITRDIPNVGEEALRNLDESGIIRLGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGAEKDLRSQAIEEEAIEKLTKDRD
                     DEIRILAESIKKDIARLVVGKTAAGRIVDVKKKTLLKKGEKITRELLQEIPFMYWKDV
                     TLAEHDGSEEKLANLLSVMERKIDFVKARFEEKLEKIKASDELPPGVIKMVKVYVAIK
                     RKLSVGDKMAGRHGNKGVLSRILPEEDMPYFSDGSTVDIILNPLGVPSRMNVGQILET
                     HLGWAAKSIGEGLNELLEKDHHLQQIKSELRKTYSSPDFDEFLEKASAEELKRFVGRL
                     KKGMLVASPVFDGCPEPQIRKLLDTAGLPENGQAVLFDGRTGEPFDQEVTVGIIYMLK
                     LHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGASYTLQEFLT
                     VKSDDVAGRTRIYESIVKGEHTFEPGLPESFNVLVKELQSLGLDVELVEEE"
     gene            3110..7216
                     /gene="rpoC"
                     /locus_tag="PLQ77_06725"
     CDS             3110..7216
                     /gene="rpoC"
                     /locus_tag="PLQ77_06725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPL10406.1"
                     /translation="MEDVFSYFEKPKNPIRFNAMKISIASPEKILSWSNGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNVMDLTLKELEKVLYFESWIVLDPKETPLKKKELL
                     TDEEFIEAKEQYGETGFVAGIGAEAVRQLLEEIDLEKLYEELRAEVVTSLSDTKKKKI
                     VKRLKVVEALRKSGNKPEWMVLTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPDLRLHQCGLPKKMALELFKPFVYNRLLEKGYV
                     TTVKSAKKMVERETSEVWDAVDEVVREYPVMLNRAPTLHRLGMQAFEPVLIEGKAIRL
                     HPLVCTAFNADFDGDQMAVHVPLSIEAQTEARVLMMSTNNILSPANGKPIIIPSQDIV
                     LGIYYLTRAKKDVKGEGMIFSGPDEVRSALDAGAIDLHARIKVRIESELKETTAGRII
                     LYEILPAEIGFDAINKLMNKKELANLIDHCYRVCSDKTTVLLADRIKDLGFKYATISG
                     LSFAVGNMIIPENKKNIVAQADKDVLKIQKQYMDGLITDGERYNKVIDIWAQATEKIA
                     SEMLTGISTEEVAGSDGKKRRIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSG
                     EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIIT
                     EQDCETVDGLYVSALMEGGEIIETAGERVLGRVALQEIRDPFTEEVIVKANEAIDETL
                     AEKIDRAGIERVNIRSVLTCRAKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEP
                     GTQLTMRTFHIGGTAKFDEHSTLEARHDGRVKFVSLNTAKTKENDYVVMNRHGEIHVL
                     DEQNRSRGKYTVPYGAHIKVGDESFVKRGQLIAEWDPFSIPILAEVDGIIKYGDIIEG
                     KTMQEQVDEVTGLSRKVIVEFKSGDLRPRVSIKDSKGKTAKIVGSNATARYFLSVGVN
                     IVVSEGDEVRAGDILAKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEIDGLVA
                     FGKDAKGKRKIVVTPEVGEEKEYLIPKGKHISVQEGDHVFPGDALMDGVNNPHDLLAI
                     KGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVRITDPGDSNFIADEQVE
                     RYRFQEENDRVVAQGGRPAIGDPLLLGITKASLSTQSFISAASFQETTRVLTEASLAG
                     KTDYLRGLKENVIMGRLIPAGTGLVMYRKLGISVEEEEAETEPQAATE"
     gene            7322..7693
                     /gene="rpsL"
                     /locus_tag="PLQ77_06730"
     CDS             7322..7693
                     /gene="rpsL"
                     /locus_tag="PLQ77_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPL10407.1"
                     /translation="MPTISQLVRKGRAKIKRKTDAPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVSSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DAVGVADRKQSRSKYGAKKPS"
     gene            7710..8180
                     /gene="rpsG"
                     /locus_tag="PLQ77_06735"
     CDS             7710..8180
                     /gene="rpsG"
                     /locus_tag="PLQ77_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPL10408.1"
                     /translation="MPRRREIEKREILPDPKYSNKLVAKFVNSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVEFFEKAVNNVKPMIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLAAELIDAANNRGGAIKKRESVHKMAEANKAFAHYRF"
BASE COUNT         2039 a         2276 c         2489 g         1457 t
ORIGIN      
        1 gaagacctgg acaacgcctc cattcgcgac acgctgctca tggaccgcat cgacagcgag
       61 gaagaggcga tcatggagat ctaccgcagg cttcgcccca gcaatccgcc caccccggaa
      121 accgcgacga agtttttcaa aagcctcttt tttgaactgg aaacctacga tttgtcggat
      181 gtcggccgcg ccaagatgaa ctacaaactg aggctgaacg tgccgacgga tttgacggtg
      241 ctgcgcaacg aagacattct ggcttccgtg aaatatctga tcgatttgaa aaacggcgtg
      301 ggcgagtgca gcgtggacga tatcgatcac ctggggaacc gccgcgttcg ctccgtgggc
      361 gaactgatcg aaaaccagta ccgcatcggt ctggtgcgga tggaacgggc gatcaaggaa
      421 aaaatgagcc tgcaggacat cgaaaccatg atgccccatg acctgatcaa cgccaagccg
      481 gtgtcggccg tggtgaatga atttttcggc tccagccagc tttcccagtt catggaccag
      541 accaatccgc tgtcggaaat cacgcacaag cgccgcctgt cggcgctggg gcccgggggg
      601 ctcacccgcg agcgcgccgg gtttgaagtc cgcgacgttc attccacgca ctacggccgc
      661 atttgtccga tcgagacgcc ggaaggcccc aacatcgggc tgattgtgtc gctttccacc
      721 tacgcccgcg tcaacgaatt cggcttcatc gaaagtccgt accgcctggt cgagaacggc
      781 cgggtgctcg atgaagtgaa atacatgacg gccatcgaag aggaaaacca gctcatctcc
      841 ccggcggacc ggccgctcga caagaagggg aaatttgagg aagaactgat ttcggtccgc
      901 aaaggcggcg actttgtttc ggccacgccg gaagagatcc ggatgatgga cgtagccccc
      961 gatcagcttg tttccgtggc ggcggccctg attccgtttc tggagcatga cgacgccaac
     1021 cgcgcgctca tggggtccaa catgcagcgc caggccgtgc cgctcatgtg ccccgaggtg
     1081 ccggtggtcg gcacgggcat ggaatcggtc gtggcccgcg attccggcgc cgtcatggtg
     1141 gccaaacggt ccggcgttgt cgaaagcgtg gacgcttcgc gcatcgtggt gcgcgccgac
     1201 aagcctgaag aagacggcct ggacaccggg gtggacattt acacgatgtc caagtatcag
     1261 cgatccaatc aggacacctg ctttaaccag aagcccatta tttccatcgg cgaccgcgtc
     1321 cggaagagag acattctggc cgacggtccc gccaccgaca acggcgaact ggcgctgggc
     1381 cggaacgtga tggtggcctt catgtcctgg ggagggtaca actacgagga ctcgattctg
     1441 gtcagcgaaa gaatcgtcaa agaagatatt tatacctcga tccatattga agaatttgaa
     1501 accatggccc gcgacaccaa gctgggcaag gaagagatca cgcgcgacat tcccaacgtc
     1561 ggagaggagg cgctccgcaa cctcgacgag agcggcatca tccggctggg cgtgtccgtc
     1621 aagcccggcg acatcctggt cggcaagatc acgcccaagg gggagacgca gttgtccgcc
     1681 gaagaaaaac ttcttcgggc gattttcggc gaaaaagcca gcgatgtgcg cgatacgtcg
     1741 ctccgcgtgc cgcccggcgt ggaaggcacg gtcatcgacg ccaagatttt cacgcgcaag
     1801 ggcgccgaaa aggatctgcg ctcgcaggcg atcgaagaag aggcgatcga aaagctgacg
     1861 aaagaccgcg acgacgaaat ccgaatcctg gcggaatcca tcaagaagga catcgccagg
     1921 ctggtggtcg gcaaaacggc ggccggcagg atcgtggacg tgaaaaagaa aacccttctc
     1981 aaaaaaggcg aaaaaattac ccgggaactc ttgcaggaga ttcctttcat gtactggaag
     2041 gacgtcacgc tggcggagca cgacggttcg gaggaaaaac tggcgaacct gctttccgtg
     2101 atggaaagaa aaatcgattt cgtgaaggcc cgttttgagg aaaagctgga gaagatcaag
     2161 gccagcgatg aactgcctcc cggcgtcatt aaaatggtga aagtttatgt cgccattaaa
     2221 aggaaactgt ccgtcggcga caaaatggcg ggccgccacg gcaacaaggg cgtgttgtcc
     2281 aggattcttc cggaagagga tatgccctat ttttcggacg gctccacggt cgatattatc
     2341 ctcaatccgc tgggcgttcc ctcccgaatg aacgtcggcc agatcctcga aacccatttg
     2401 ggctgggcgg ccaaatccat cggcgagggc ctgaatgaac tgctggaaaa ggaccaccat
     2461 ctccagcaga tcaaaagcga attgagaaaa acctactctt ctccggattt tgacgagttt
     2521 ctggaaaagg cgtccgccga agagctcaag cggtttgtcg gccgcctgaa aaaaggcatg
     2581 ctggtggcca gccccgtttt cgacggctgt cccgaaccgc aaatccgcaa gctgctagac
     2641 acggcggggc ttccggagaa tggacaggcg gtccttttcg acggccggac cggagagcct
     2701 tttgatcagg aagtcacggt cggcatcatc tacatgctga agctgcacca cctggtggac
     2761 aataaaattc acgcccgctc catcgggccc tattcgctgg tgacccagca gccgctgggc
     2821 ggcaaggcgc agttcggcgg tcagcggctg ggagaaatgg aagtatgggc catggaggcc
     2881 tacggggctt cctacaccct gcaggaattt ttgacggtca agtccgacga cgttgccgga
     2941 cgaacgcgga tttacgaatc catcgtcaaa ggggagcata ccttcgagcc gggcctgccg
     3001 gaatccttca acgtgctggt gaaggagctg cagagcctcg ggctggacgt ggaactggtc
     3061 gaggaagagt agccccgcta agatgtttta aaaaacagga gaaaaatctg tggaagacgt
     3121 tttcagctat tttgaaaaac ccaaaaatcc gattcgcttc aatgccatga agatttcgat
     3181 cgcctcgccg gaaaagatcc tttcctggtc caacggcgaa gtcaagaagc cggagacgat
     3241 caattaccgg accttcaagc cggagcgcga cggcctgttc tgcgccaaga ttttcgggcc
     3301 ggtcaaggac tatgagtgtc tttgcgggcg ctacaagcgg atgaagcacc ggggcgtggt
     3361 ctgcgaaaaa tgcggcgtcg aagtcattca gtccaaggtg cgccgcgagc ggatgggcca
     3421 cattaccctg gccacgccgg tcgcgcacat ctggtttttg aaaagtctgc ccagccgcat
     3481 cggcaacgtg atggatctga cgctcaagga gctggaaaaa gtgctctact ttgagtcctg
     3541 gattgtcctg gacccgaaag agacgccgct gaaaaagaag gagcttctca ccgacgagga
     3601 gtttatcgaa gccaaggagc agtacgggga aaccggattt gtcgccggca tcggcgcgga
     3661 agccgttcgt cagcttctgg aagaaatcga cctggaaaaa ctctacgaag aattgcgggc
     3721 ggaagtcgtc acgtcgcttt ccgacaccaa aaagaaaaaa atcgtcaaaa ggctcaaagt
     3781 cgtcgaagcc ctgcgcaagt ccggcaacaa gccggagtgg atggtgctga ccgtcctgcc
     3841 ggtcattccg ccggatttgc ggcccctggt tccgctggac ggcggccgtt tcgccacgtc
     3901 cgacctcaac gacctgtacc ggcgcgtaat caaccggaac aaccggctca aaaggctcca
     3961 ggagctcaac gcgccggaaa tcattatccg caatgaaaag cgcatgctgc aggaagccgt
     4021 ggacgttctg tttgacaacg gccgccgcgg acgcgcgatc accgggacca acaagcgccc
     4081 gctgcgctct ctctcggata tgctcaaagg caagcagggg cgcttccgcc agaacctgct
     4141 gggcaagcgc gtggactact ccgggcgttc cgtcatcacg gtgggccccg acctgcggct
     4201 gcatcagtgc gggctgccca aaaagatggc gctcgaactc ttcaagccgt tcgtctataa
     4261 ccgccttctg gaaaaaggct acgtgacgac ggtcaaaagc gccaagaaga tggtcgaacg
     4321 ggaaacctcc gaggtctggg acgccgtgga cgaagtcgtg cgggaatacc ccgtgatgct
     4381 caaccgcgcg cccacgctgc accgcctggg catgcaggcg ttcgaaccgg tgctgatcga
     4441 aggcaaggcg atccgcctgc atccgctggt ctgcacggcc ttcaacgcgg actttgacgg
     4501 cgaccagatg gccgtccacg tgccgctgtc catcgaggcg cagaccgaag cgcgcgtgct
     4561 gatgatgtcc acgaacaaca tcctctcccc ggccaacggc aagccgatca tcattcccag
     4621 ccaggacatc gttctgggga tttactatct gacccgggcg aaaaaggacg tgaagggcga
     4681 gggcatgatt ttttcgggcc cggacgaagt gcgctccgcg ctggacgcgg gggcgattga
     4741 cctgcacgcg cggatcaagg tccgcatcga gtcggagctg aaggaaacga cggccggccg
     4801 catcatcctc tacgagattc ttcccgcgga gatcggtttt gacgcgatca acaagctgat
     4861 gaacaagaag gaactggcca acctgatcga ccactgctac cgggtctgca gcgataaaac
     4921 cacggtgctg ctggccgacc gcatcaagga tctgggattc aagtacgcga cgatttccgg
     4981 cctgtcgttc gccgtcggga acatgatcat tcccgagaac aagaaaaaca tcgtggcgca
     5041 ggccgacaag gatgtgctga aaatccagaa gcagtacatg gacggtctga tcacggacgg
     5101 cgaacggtac aacaaggtga tcgacatctg ggcgcaggcg accgaaaaga tcgcctccga
     5161 aatgctgacc ggcatcagca cggaagaagt ggcgggttcc gacggcaaaa agcgcagaat
     5221 cgagagcttc aacccgattt acatgatggc ggattccggc gcccgcgggt ccggacagca
     5281 gcttcggcag ctggccggca tgcgcggcct gatggcgaag ccctccggcg agatcatcga
     5341 aacgccgatc acggccaatt tccgtgaagg cctgacggtt ctgcagtact ttatttccac
     5401 gcacggcgcc cgcaagggtc tggcggatac ggccctgaaa acggcgaact ccggctacct
     5461 gacccgaaga ctggtcgatg tggctcagga ctgcatcatc accgagcagg attgcgaaac
     5521 ggttgacgga ctgtatgtgt ccgcgctcat ggaaggcggt gaaatcattg aaaccgccgg
     5581 cgagcgtgtg ctgggccgcg tcgccctgca ggaaatccgg gaccccttca ccgaagaagt
     5641 gatcgtcaag gccaatgagg ccatcgatga aaccctggcc gaaaaaatcg accgagcggg
     5701 cattgagaga gtcaacatcc ggtccgtttt gacctgccgc gccaaacacg gcgtctgcgc
     5761 catgtgctac gggcgcgacc tggcccacgg ccatctggtg aacatcggcg aggcggtcgg
     5821 catcgtagcc gcccagtcca tcggcgaacc cggcacgcag ctcaccatga gaacgttcca
     5881 catcggcggc acggccaagt ttgacgagca ctccaccctg gaggcgcgtc atgacggccg
     5941 ggtcaaattc gtgagcctga atacggccaa gacgaaagaa aacgattacg tggtcatgaa
     6001 ccgccacggc gaaatccatg tgctggacga acagaaccgg agccgcggca aatataccgt
     6061 tccctacggc gcccacatca aagtgggaga cgaaagcttt gtgaagcgcg ggcagctgat
     6121 cgccgagtgg gaccccttct ccataccgat tctggccgaa gtggatggaa ttattaaata
     6181 cggcgatatt attgaaggaa aaaccatgca ggaacaggtc gatgaggtta ccggcctctc
     6241 cagaaaggtg attgtcgagt ttaagagcgg cgatttgcgg ccccgcgtgt ccatcaagga
     6301 cagcaagggg aagactgcca agatcgtcgg gtcgaacgcg acggcccgct atttcctgtc
     6361 ggtgggcgtc aatatcgtcg tctccgaagg cgatgaggtt cgcgcgggcg acattctggc
     6421 gaagattccc agggaaacaa caaaaaccaa ggatatcaca ggcggtctgc cgagggtcgc
     6481 ggagctcttt gaagccagaa agcctaagga tttcgccgtg atcagcgaaa tcgacggtct
     6541 tgtcgccttc ggcaaggatg ccaagggcaa gcgaaaaatt gtcgtgacgc ctgaagtcgg
     6601 cgaggaaaag gaatacctga ttcccaaggg caagcacatc agcgtccagg agggcgacca
     6661 cgtgttcccc ggcgacgctt tgatggacgg cgtcaacaat ccccatgacc ttctggccat
     6721 caagggcgaa aaagaactgg cccgttacct ggtggatgaa gttcaggaag tttatcgttt
     6781 gcagggcgtc aagattaatg ataaacacat ggaagttatc gtccgccaaa tgctgcgccg
     6841 cgtccggatc accgatcccg gcgactccaa tttcattgcc gacgaacagg tagagcgcta
     6901 tcgtttccag gaggaaaacg accgggttgt cgctcagggc ggccgtcccg ccatcggcga
     6961 tccgctgctt ctgggaatca ccaaagcgtc gctttccacg cagagcttca tatcggcggc
     7021 ttccttccag gaaaccaccc gggtgctgac ggaggcttcg ctggcgggca agacggacta
     7081 cctgaggggc ctcaaggaga acgtcattat ggggcgtctg attccggccg gaaccggcct
     7141 ggtcatgtac cgcaagctgg gcatcagcgt cgaggaagag gaggcggaaa cggagccgca
     7201 ggcggcgacg gaataaacga aaaaaaagca taaaagagct tgacagaaac agcttgaatt
     7261 gatagttacc gcagatttgc tactcccgtc aaaaacggcg ggacagcgtt cgggagggaa
     7321 gatgccgaca attagtcaat tggttcgaaa aggtagagcc aaaatcaaga gaaaaacgga
     7381 cgcgccggcg ctggccggtt cgccgcagcg gcgcggggtt tgcgtgagag tgtacacgac
     7441 cacgcccaaa aagccgaatt cggcgctgcg caaggtggca agggtccggc tgaccagcgg
     7501 gtatgaagta tcatcctaca ttcccggcat cggccacaac ctgcaggagc actctgtagt
     7561 gctggttcgc ggcggcagag tgaaggattt gcccggcgtc cggtaccata tcgtccgggg
     7621 tacgctggat gccgtcgggg ttgccgaccg caagcagagc cgatcaaaat acggagcgaa
     7681 aaagcccagt tagatgaagg attgaaaaaa tgccacgaag acgggagatt gaaaaaaggg
     7741 agattctgcc ggatcccaaa tacagcaata aactggttgc caagtttgtg aattcattgc
     7801 tcaaacgtgg taaaaagagc gtcgcggaaa gcattctgta cggcgctttt gacattattg
     7861 aaaagcgcgt caaggaacag ccggtggaat tcttcgaaaa agcggtcaac aacgtcaagc
     7921 cgatgattga agtgaagtca aggcgcgtag gcgggtccac ctatcaggtg ccgacggaag
     7981 tcgttcccgc ccggcgcacg gctctggcga tccgctggct catttccaac gcgcaggagc
     8041 gcaccgaaaa aaccatgcgg gaaaagctgg cggcggagct gattgacgcg gccaataacc
     8101 ggggcggcgc catcaagaaa agagaatccg tgcataaaat ggcggaggcc aacaaagcgt
     8161 tcgcgcatta tagattttaa ataaacttat aaaacgacaa ggaaaacaac cattcaaagt
     8221 taaggaggaa aggtgagatg gcaaagaaaa aatttgaaag g
//
LOCUS       DAOPAF010000069         8251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_8016_length_8251_cov_42.991581, whole genome shotgun sequence.
ACCESSION   DAOPAF010000069 DAOPAF010000000
VERSION     DAOPAF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8016_length_8251_cov_42.991581"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1390)
                     /locus_tag="PLQ77_06740"
     CDS             complement(2..1390)
                     /locus_tag="PLQ77_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPL10409.1"
                     /translation="MMNGKKGKAQAEKKRATGEANKTPEKYFVVDTSALEYGIDVFEK
                     LRQGGRNCVLMPYATWEELDSHKTGNDFKADIARSVIRNVRNLFRAQDRSVRFFSYDF
                     TAAKDGGLKTDKNDHCIIATALHARQKLPAEKVILVTQDAALETLALNFGMEVQSVKM
                     DETEVKEQHAPRITVNLQKQWIADKTFPLKLPEARLADKLGQIRLNEGIVCYCERDGA
                     WGPAFAAVRKGDHFRIVDKDIHLLGVRPRSNNGEVNWGQYIAMDLLRDASVPLVALTG
                     KAGTAKTFLSLLAAFEQASRYRRILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIY
                     DNINAIKELVDDKMKEKINEWLTNGKIEMMDLDKIRGRSIQKCFVIVDEIQNLPPSHV
                     KAIATRGGEGTKMVFTGDFSHDQIDVPWLDERSNGLSVLNARMAGSRLFGSVHLDQAI
                     RSELTKEILERW"
     gene            1632..3221
                     /locus_tag="PLQ77_06745"
     CDS             1632..3221
                     /locus_tag="PLQ77_06745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPL10410.1"
                     /translation="MNEKNLILAVDVGTTETKAVLVSPEGYIDSRQSTCCLCYPDKSC
                     VEQSPEEISDAVYHSSREVLAAHPDLAGRLAGVVFTSQMQNTVPVDRNGKPLMNMLSW
                     MDERASCFTQEDMFRGWIKIEGYPVLKLLKFMRITGGAPGKTGKDTVCKLAWMKKNKP
                     DLYASVHKFLDAKDYAIFLATGNYVTSYDMANVTWLMDTRNKDIRKWVWSEEIHKMYG
                     LDSRKMPELRASTAIAGSVTSDFAAKTGVPAGLPVVTGCGDLLASAIGSGAIEMGQLH
                     VNIGTAGWVATHHPGTAIDTQHYVGAIVSGIPGKFLLISKQETLGAALDWLKGMLYPS
                     ELLPGKSTAEIYRMMDAEAALSPSGANGVLLTPWLFGERSPINNPDLRGQIFNISMNT
                     TRADLCRSVMEGVALNIRWGMEYVEKMSLKKSRPTTDEVRLIGGASKSDIWCRIFADV
                     LQKPIVQMKNAQMASAQGAAAVAMVAFGIYRDFSEIDRMLQKGKVFKPNPATAALYEK
                     LYRQYQALYKNNKGAFRELNH"
     gene            3234..4841
                     /locus_tag="PLQ77_06750"
     CDS             3234..4841
                     /locus_tag="PLQ77_06750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPL10411.1"
                     /translation="MNRFIDSCQDETFDMLVIGGGITGACVAYDAACRGLKVALVEKG
                     DFGAATSAATSKVIHGGLRYLKTFEFGLVRESLKERRVMGNIAPNFIYPTAFMFVNPT
                     FVMKIGLCLYDWIAFDKKFTWDKSKKIPNHYSLSREEVIRQAPNARREGLKGAWVYYD
                     SLSIFPERLTLAFLKSAVHYGAKVSNHARMEGFVMEGGRVSGIVVRDLLNNQIHTLKG
                     KLTVNCAGPWADMVLKMAGKGAAQDSIRRSEGIHLIVRNLVGKQAVGTMTRAGHFFLI
                     PWRNYSLIGTTDKEYPGTPDDYHVTGKSIQELLDTVNRDFGNDNPIRYGDVLYAYGGL
                     RPLLGSGGDIHTITRKYEIYDHADEGLPGLITAEGGKYTTSRNLAQNVLKAAEKRLGK
                     LQPCLTHKKRLVGSEIDDMEGFIEAVKRENRDFSEKTVVYLGRNYGTEYDRVLKMARQ
                     DQALADVLNDDGEILAQAVYAAREEMARTLADVVLRRTGICTLGHPGKEILNKVAAAV
                     ARELKWDGERTEREVEEVEKILKVPAA"
     gene            complement(4857..5924)
                     /locus_tag="PLQ77_06755"
     CDS             complement(4857..5924)
                     /locus_tag="PLQ77_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10412.1"
                     /translation="MNVDLHNDPDLPQAWAPWFSHEYITYHAVRALDGLTFLPPVAVK
                     PRPPFADKVWPVGPKHIDIYKESYQERRRLDPVWSFDYKKRNGDFALAPPSGKTIEPW
                     KVLVIYSTEPDLYLDHDLAVHKNQKITGGSHGWRHMEFKVLTSTFGAAPKSFLVHQDF
                     SGLAFENGNDYWGWRYLSRCGHYLADLGSPFHVKALPSCFLMKNIFSGQTLFRTASAV
                     HQGYEVYVERRFREGFEPFGQALARGASEAKNAAGNVRHRLPAYMEKARKKHNEVFYF
                     FLDQYGRDLLEVFSRVDPGGPTETASQTNQCSADAARVIFRDGALPALSFLDGITAGI
                     LEDVGLMLGMLFQSFAAAKAR"
     gene            complement(5983..7323)
                     /locus_tag="PLQ77_06760"
     CDS             complement(5983..7323)
                     /locus_tag="PLQ77_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HPL10413.1"
                     /translation="MAKKKVPADCKEIGPFADKPIALPEGIDMENHVIATYWVQADKN
                     MDMAIMGQFLAIEQTTGTWVPVPGETPEIRAGHVAKVIGVYEAPYYEYGVPPEAANRQ
                     YVLQIAFPAANVEDQLPMMLTAVIGNISLVPNFKLLDLRLPKATLDQYNGPKFGADGW
                     WKALGLKKGRPLLNNMIKPCSGYPLEVGAKLFREAAMGGCDVIKDDELIASMRYNDAV
                     KRVRAYMKIEKEVYEETGEHTLYAVNVTDRLPRMFDLARRCVDAGANSIMVNYLSVGP
                     EAMRALADDPGISVPILGHMDLAGAYFMSPVQGIASPLILGKFARLCGADSVVLPFSL
                     GGKAMYMHDRFMSTVRNLTYPMGGLKPSLPMPSGGITPANVADIVNTLGKDTMIGSGG
                     GIHAHPGGPRAGARALRQAIDAAVRGIGLEEYAKEHEELGVALGVWNKKTDFKI"
     gene            7570..>8251
                     /locus_tag="PLQ77_06765"
     CDS             7570..>8251
                     /locus_tag="PLQ77_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10414.1"
                     /translation="MQNEDVLARIHLNAVLSNLEILAREDKEASAIAGTWNGGILLMV
                     GLQGPKVTLEMREGAVRVATGRTGNPGIVLLFPTARMLNNLFTGAGRGFPVPVKGLFR
                     IKGLKAFAGLAKRMEAVLKGADAPRKIKARLLLNTVGRTVAVLAASEPESQKAAFGLR
                     GVAELRIKDSEAVHIIFTEQGATAGSGRAPNPDLVMEFSSEEVFLDLTEDKVDVFAAI
                     CLEDIRIQG"
BASE COUNT         1734 a         2298 c         2485 g         1734 t
ORIGIN      
        1 tttaccatcg ctccagaatc tccttggtca gctccgaacg gatggcctga tccaggtgca
       61 cgctgccgaa gaggcggctt ccggccatcc tggcgttcag gacggaaagc ccgttggagc
      121 gctcgtccag ccacgggacg tcaatctgat cgtgggaaaa gtcgccggta aagaccattt
      181 tggttccttc gccgccgcgc gtcgcgatgg ctttcacatg cgatggcggc agattctgaa
      241 tctcgtcgac gattacgaag catttctgaa tggaacggcc ccggattttg tccaggtcca
      301 tcatctcgat ttttccgttc gtcagccact cgttgatctt ctccttcatt ttgtcgtcca
      361 ccagttcctt gatcgcgttg atgttgtcgt agatcgggtg catccagggc ttcatcttgg
      421 cgtcgatgtc gcccggcgaa taccccagcc gatccttgtt gcccagctgg atggtggcgc
      481 ggctgaccag aatccggcgg tagcgggagg cctgttcaaa ggcggccaga agcgacagaa
      541 aggttttggc ggttcccgcc ttgcccgtca gcgcgaccag cgggacggat gcgtcgcgca
      601 gcagatccat ggcgatatac tggccccagt tgacctcccc gttgttggac ctgggccgga
      661 cgcccagcag atgaatgtcc ttgtccacga tgcggaagtg gtcgcctttg cgcaccgcgg
      721 caaaggcggg cccccaggcc ccgtcgcgct cacagtagca gacgatgccc tcgttgagcc
      781 ggatttggcc cagcttatcg gccagacggg cttcgggaag tttcagcggg aacgttttgt
      841 cggcgatcca ttgcttttga agattcacgg tgatccgggg cgcgtgctgc tccttgacct
      901 ccgtttcatc cattttgacg gattggacct ccatgccgaa attcagggcc agcgtttcca
      961 gcgccgcgtc ctgcgtgacg agaatgactt tttcggccgg cagcttttgc cgggcgtgaa
     1021 gcgcggtggc gataatgcag tggtcgttct tgtcggtttt caggcctccg tctttggccg
     1081 cggtgaagtc ataagagaaa aaccggacgg aacggtcttg ggcgcgaaac aggttgcgga
     1141 cattgcgaat gacgcttcgg gcaatgtccg ctttgaaatc attgccggtt ttgtggctgt
     1201 ccagttcctc ccaggttgcg taaggcatca aaacgcagtt gcgtcctccc tggcgcagtt
     1261 tttcaaacac atcgattccg tattccaggg cgctggtgtc cacgacaaaa tacttttccg
     1321 gtgttttatt cgcctctccg gtcgctctct tcttctccgc ctgggctttg ccttttttcc
     1381 cgttcatcat gatgtggttc cccgcttgaa gagtttgaag ttatggaatg cagaatgtgt
     1441 gaaggcgcac ttccaatgcc tgcaccggct gaattgttgg gtgccattgt accaaattcg
     1501 gaaatttcga caagggaaag ttgaagaggg cgctttttgg gcgacaaaaa gggctgaact
     1561 ctgcggcaac ttgtggtaca agcatcgcgg ttcaatcatc agtcatcttc ccgggacaag
     1621 gaggagccag catgaatgaa aagaatctga tccttgcggt ggatgtcggc accacggaaa
     1681 ccaaagcggt gctggttagc cctgaagggt acatcgattc ccgtcagtca acctgctgtc
     1741 tttgttatcc ggacaagtcg tgcgtcgagc agtcgcccga ggaaatatcg gacgccgtct
     1801 atcattccag ccgggaggtt ctggccgcgc atcccgatct ggccggaagg cttgccggcg
     1861 tcgtttttac cagccagatg caaaacaccg ttccggtgga caggaacggg aagccgctga
     1921 tgaacatgct ctcctggatg gatgagcgcg cgtcctgctt cacgcaggaa gacatgttcc
     1981 ggggatggat caagatcgaa ggctaccccg ttctgaagct gctgaagttc atgagaatca
     2041 ccggcggcgc tcccggaaaa accggaaagg atacggtctg caagctggcc tggatgaaaa
     2101 aaaacaagcc ggatctgtat gcttcggtcc ataaatttct ggacgcgaag gactacgcca
     2161 tttttctggc caccggcaat tatgtgacgt cctatgacat ggccaacgtc acctggctca
     2221 tggatacgcg caataaggac atcaggaagt gggtctggtc ggaagagatt cacaaaatgt
     2281 acgggctcga ttcccgaaaa atgccggagc tcagggcgtc caccgctatt gccggttccg
     2341 tcacctcgga ttttgccgca aaaaccggcg tcccggcagg ccttcccgtc gtgaccggct
     2401 gcggcgacct tcttgcctcg gcaatcggct ccggcgccat cgaaatgggg caactgcatg
     2461 tgaatatcgg cacggccggc tgggtcgcca cgcaccatcc cgggacggcg atcgacacgc
     2521 aacactacgt cggcgccatc gtgtcgggca ttccgggaaa atttcttctg atcagcaagc
     2581 aggaaaccct gggcgcggcg ctggactggc tgaagggcat gctgtaccct tctgagctcc
     2641 tgccgggaaa atctaccgcc gagatttaca ggatgatgga tgcggaagcg gccctgtcgc
     2701 cttccggcgc gaatggcgtt cttctaaccc cgtggctttt cggcgagcgg tcccccatca
     2761 acaatccgga cctgcgcggg cagatcttca atatcagcat gaacacgacg cgggccgatc
     2821 tctgccggtc ggtcatggaa ggggttgccc tgaatattcg ctggggcatg gaatacgtgg
     2881 aaaaaatgtc cctgaaaaag agccggccga ctacagacga agtccggctg atcggcggcg
     2941 cttcgaaaag cgacatctgg tgccggattt ttgcggacgt tttgcaaaag ccgattgtac
     3001 agatgaaaaa cgcccagatg gcctccgcgc agggcgcggc cgccgtagcg atggtggctt
     3061 tcggcattta cagggatttc agcgagattg accggatgct gcaaaaaggc aaagtcttta
     3121 agccgaatcc cgccacggcc gctctgtacg aaaaactata ccggcagtat caggcgctgt
     3181 ataagaacaa caaaggcgct tttcgggaac tcaatcattg aggagtatgc gccatgaatc
     3241 gtttcatcga tagctgccag gacgaaactt tcgatatgct ggtgatcggg ggcggcatca
     3301 cgggcgcctg cgtagcgtac gacgcggcct gccgcggcct gaaagtcgcg ctggtggaaa
     3361 aaggcgattt cggcgcggcg acctccgccg ccacgtccaa ggtgatccac ggcggcctgc
     3421 ggtatctgaa aacctttgaa ttcgggctgg tgcgcgagtc gctcaaagag cgcagagtca
     3481 tgggaaacat cgccccgaat ttcatctacc ccacggcctt catgttcgtc aatccgacct
     3541 tcgtcatgaa gatcggcctg tgcctgtatg attggattgc atttgacaag aaattcacct
     3601 gggacaagag caagaaaatc cccaaccact atagcctttc cagggaagaa gttatccggc
     3661 aggcgcccaa cgccaggcgg gaagggctta agggcgcctg ggtgtattat gacagcctca
     3721 gcattttccc cgagcgcctg acgctggcct ttttgaaatc ggccgttcat tacggcgcca
     3781 aagtctccaa tcacgcccga atggaagggt ttgtcatgga gggcggcagg gtttccggca
     3841 ttgtagtgcg ggacctgctg aataaccaaa tccataccct gaaggggaag ctgaccgtca
     3901 actgcgccgg gccctgggcg gacatggtgc tgaaaatggc cggaaaaggg gcggcgcagg
     3961 attccatccg ccgctcggag gggattcatt tgattgtcag gaatctggtg ggcaaacagg
     4021 ccgtcggcac gatgacccgg gccggccatt tcttcctgat tccctggcgg aactattcgc
     4081 tgatcggcac gacggacaag gaatatcccg gaacgccgga cgattatcat gtgaccggta
     4141 agagcattca ggaactgctg gacacggtga acagggattt tggcaacgac aatcccatcc
     4201 gttacgggga tgtcctttac gcgtacgggg ggcttcggcc actgcttggt tccgggggcg
     4261 atatccatac catcacgcgg aaatacgaga tttacgatca tgcggatgag gggcttcccg
     4321 gcctgatcac ggccgagggc ggcaaataca cgaccagccg gaatctcgcg caaaatgttt
     4381 taaaagcggc ggaaaaaaga ctgggcaagc ttcagccgtg ccttacgcac aaaaagcggc
     4441 tggtcggcag cgagatcgac gacatggaag gattcatcga agccgtcaag cgggaaaacc
     4501 gggatttttc agaaaaaacc gtcgtgtatc tcggccggaa ttacggcacc gagtacgaca
     4561 gggtgctgaa gatggcccgg caggatcaag ccctggcgga tgttttgaat gacgacggcg
     4621 agatcctggc ccaagccgtc tatgccgccc gcgaggaaat ggcccgaacg ctggccgacg
     4681 tggttttgcg cagaacgggc atttgcacgc tgggccatcc ggggaaggag atcctaaaca
     4741 aggtggccgc cgcagttgcc cgggagctga agtgggacgg agaaagaacg gagcgcgagg
     4801 tcgaagaggt ggaaaaaatc ctgaaggttc cggcggcatg acgctgtgtg aacgatttac
     4861 ctcgctttgg ccgccgcaaa ggattgaaac agcattccca gcataaggcc gacgtcctcc
     4921 agaatcccgg cagtgatccc gtcgaggaag gaaagcgccg gaagcgcgcc gtctctgaaa
     4981 atcaccctgg ccgcgtcggc ggagcattga ttggtctgtg aggccgtctc tgtggggccg
     5041 ccgggatcta cccggctgaa aacctccagc aggtcccggc cgtactgatc gaggaaaaaa
     5101 taaaaaacct cgttgtgctt ttttcttgcc ttttccatgt aagccggaag gcgatgccgc
     5161 acatttcctg ccgcattttt cgcttcgctc gctccccgcg caagagcttg cccgaacggc
     5221 tcaaaccctt cacggaagcg gcgttcaaca tacacctcat agccctgatg caccgcggaa
     5281 gccgtccgga acagcgtctg tccggagaag atgtttttca tgaggaaaca agagggcagc
     5341 gccttcacgt gaaaggggct gcccagatcc gccaggtaat ggccgcaccg ggacaggtag
     5401 cgccaccccc agtagtcgtt gccgttttca aaagcaagcc cgctgaaatc ctgatgaacg
     5461 aggaaggatt tgggcgccgc gccgaaggtc gaggtcagca ccttgaattc catgtggcgc
     5521 cagccgtggg acccgccagt gattttctga tttttgtgga cggccagatc gtggtccaga
     5581 taaagatccg gctccgtgct gtaaatcacc agcactttcc aaggctcgat cgttttcccc
     5641 gaaggcggcg ccagggcaaa atcgccgttt cgtttcttgt agtcgaaaga ccataccgga
     5701 tccagtcgcc gtcgctcctg atagctttcc ttgtaaatat cgatgtgctt cggaccgacg
     5761 ggccagacct tgtcggcgaa agggggacgg ggcttcacgg cgacgggcgg caggaaggtg
     5821 aggccgtcca gcgcccggac ggcgtggtac gtgatgtatt cgtgggagaa ccagggcgcc
     5881 caggcttggg gcaaatccgg atcattgtga agatcgacat tcaaggccgg gcatccttaa
     5941 gcgatgcggg gaaaggccgg aacctctccc cgcaaaccat agctagattt tgaaatcggt
     6001 ttttttgttc cagacgccca gcgcgacgcc cagctcttcg tgttctttgg cgtattcttc
     6061 cagcccgata ccccgaaccg ccgcgtcgat ggcctgacgg agcgcacggg cgccggccct
     6121 cgggccgccc ggatgggcgt gaatgccgcc gcccgagccg atcattgtgt ctttgcccag
     6181 cgtgttgacg atgtccgcca cgttggccgg cgtgatgccg cccgacggca tgggcagaga
     6241 gggcttcagg ccgcccatcg gatacgtcag attgcgcacg gtgctcatga aacgatcgtg
     6301 catgtacatg gccttgccgc ccagagagaa gggcagaacg accgagtctg ctccgcacag
     6361 acgggcaaat ttgcctaaaa tgagcggcga ggcgattccc tgaacgggcg acatgaagta
     6421 ggcgccggcc aggtccatgt ggcccaggat cggaacggaa atgcccggat catcggccag
     6481 ggcgcgcatg gcctccggac cgacggacag ataatttacc atgatcgagt tcgcgcccgc
     6541 gtcgacgcag cgcctggcca ggtcgaacat ccgcggcagg cggtcggtga cattgacggc
     6601 gtaaagggtg tgctcgccgg tttcctcata cacttccttt tcgattttca tataggccct
     6661 gacgcgtttg accgcgtcgt tgtagcgcat gctggcgatc agctcgtcgt ccttgatcac
     6721 gtcgcagcct cccattgccg cttcccggaa gagcttggcg ccgacttcca gcggatagcc
     6781 gctgcagggc ttgatcatgt tgttgagcag cggacggccc tttttgagcc ccagcgcctt
     6841 ccaccatccg tccgcgccga atttaggccc gttgtactgg tccagcgtgg ctttcggtag
     6901 ccgaaggtcc aggagtttga agttgggaac cagcgagatg ttgccgatga cggcggtcag
     6961 catcatggga agctggtctt cgacgttggc ggcgggaaag gcgatctgca ggacgtactg
     7021 ccggtttgcc gcctccggcg gaacgccgta ttcgtagtag ggcgcttcat agacgccgat
     7081 cacttttgcg acatgtccgg ccctgatttc cggcgtttcg ccgggaaccg gcacccaggt
     7141 gccggtggtc tgttcgatgg ccagaaactg ccccatgatc gccatgtcca tgtttttgtc
     7201 ggcctgtacc cagtaagtgg caatcacgtg gttttccata tcaatgcctt cgggcagggc
     7261 gatgggtttg tccgcgaagg gaccgatctc tttgcaatcg gcggggactt tctttttcgc
     7321 catgggatac tcctctcggt gaattttatt ttttgacggc gcggatgtcg tctgcgtccg
     7381 cggcagacga cgggatgagc cgccgggaaa cggaccggcc gatcaaccgg acggcaccgg
     7441 ttttccgggt tttctatcgc acagatcccg aaatagttca agcgcctatc cggtattgac
     7501 agggtatccc gggaagagta aaaaggaaaa cacacgtcat gcttcattgg caaaaccggg
     7561 gagggataga tgcaaaacga agatgttttg gcaagaatcc acctgaatgc ggtgctttcc
     7621 aatcttgaaa tccttgcacg ggaagacaag gaagcatcgg cgatcgccgg gacgtggaac
     7681 ggcggcatcc tgctcatggt cgggcttcaa ggccccaagg tcacgctgga aatgagagag
     7741 ggtgccgtga gggttgcgac tggccgaacc ggaaatcccg gcatcgtttt gctttttccc
     7801 accgctcgaa tgctcaacaa tctgtttacc ggcgccggcc gcggctttcc tgttcccgta
     7861 aaaggtctgt tcaggatcaa gggactcaaa gcgtttgccg gacttgccaa aaggatggag
     7921 gccgttctca aaggcgccga cgctccccgg aaaatcaaag ccaggctgct gctcaacacg
     7981 gtgggcagga cggttgccgt tcttgccgca tcggaaccgg aatcccaaaa agccgccttc
     8041 ggacttcggg gggttgcgga gcttcgaatc aaagacagcg aagcggttca catcatcttt
     8101 accgaacagg gtgcgaccgc aggaagcggc cgcgcgccga atcccgacct tgtcatggaa
     8161 ttttcctcag aggaggtgtt tcttgacctc acggaagaca aggtggatgt gtttgccgcc
     8221 atctgcctgg aagacattcg aatccagggg g
//
LOCUS       DAOPAF010000070         7620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_8728_length_7620_cov_48.077198, whole genome shotgun sequence.
ACCESSION   DAOPAF010000070 DAOPAF010000000
VERSION     DAOPAF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8728_length_7620_cov_48.077198"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLQ77_06770"
     CDS             complement(<1..662)
                     /locus_tag="PLQ77_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL10415.1"
                     /translation="MLKKEVKAIFEKSRKWGWVLEPDAQKILSLYGFKTPKFGVAATE
                     GEAVAAARKIGYPVVAKIVSPAVVHKSDVKGVVVGIKDDETLVRTLARLSKIDGFAGM
                     LIAEMVKGLELIVGAKNDFQFGPMILLGMGGVGVEIYKDVSLRMAPLKTRDADHMIQE
                     LAARKLLTGYRGSEPVNLNALKKTIVAFSHLMMDMRDVVESVDLNPVMCTAKSCIVAD
                     AR"
     gene            complement(656..2023)
                     /locus_tag="PLQ77_06775"
     CDS             complement(656..2023)
                     /locus_tag="PLQ77_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL10416.1"
                     /translation="MNLKPLFEPKTMAVIGVSASNDRHPANVIYDKNNHRYPVKVYAV
                     NPRGGSLHNETVYPRVAEIPAKIDLAVIATRAELVPDVMADCIAAGVKSATVISGGFS
                     EGGRGDLQDRIVDMAREAGLPFIGPNCLGVYVPFRVDTFFLPIERMIKPEAGGVTFVS
                     QSGGILVDHMIKFAQQGVGMAKAVSIGNKAFIREKELLAYLAHDPATKVIAYYIEGFG
                     EGEGREFVQAADVCSKPVVIMKSGKTAAGNRAVSSHTASLAGDYASFAAAVAQHNIIE
                     AVSENEMVSFCEVLSSYTRPIEGRIGIITGSGGHGAMAVDACSMAGLAVPEFSDQTKT
                     DIKNQLTESVRAIASLGNPADLTGSAVDDDFVACARVLAASPEIDCVVVLLLPYIPGV
                     SLDIGVRLSSVCRQSGKPFIAYVPHVEKYNMLIEGFELNGIPASSSIEGAVLMAKALA
                     RRQSC"
     gene            complement(2052..2906)
                     /gene="pgeF"
                     /locus_tag="PLQ77_06780"
     CDS             complement(2052..2906)
                     /gene="pgeF"
                     /locus_tag="PLQ77_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPL10417.1"
                     /translation="MFSWSNAKGIPYLQSSGLAACGFLEHAFCARKGGVSEDDYQSLN
                     MSFREGDEEFRVLQNWNKLAAAFGIPVENFLVLNQVHRDGILVIPSRGEYFTSREDLN
                     YDAIVTDRVHLAICIKTADCVPVFLADPENQVIAAVHAGWRGTAAGITAKVVRLMQNQ
                     YGSRPVDLLAAIGPSIGRCCYEVDAPAADAFRLHPDAKNFLFPSKRKDRWMLDLVQAN
                     RGQLIDCGIPDASIDASGLCTVCREDDFFSHRGSGGVTGRQINFLMLKGDPICKALLL
                     NDDLCHIH"
     gene            complement(2972..3997)
                     /locus_tag="PLQ77_06785"
     CDS             complement(2972..3997)
                     /locus_tag="PLQ77_06785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPL10418.1"
                     /translation="MSIENQESQNLSFTVDSAQAGQRLDVFLSQADPAFSRSQIKYAV
                     EEGNVTVNGREPKASQHLKAGDRVELHLEPAVEAAAAPQDIPLNIVYEDGSIIVVNKP
                     AGMVVHPAPGNPDQTLVNALLFHCRDLSGIGGFLRPGIVHRLDKETSGLIVAAKSDEA
                     HRKLSAQFEKHDVHKKYIALCWGDVKGASGEIVLPVGRHPKDRKKMSTKSRHGKDALT
                     LWKVRERYGTATLLDIEIKTGRTHQIRVHLAERGYPVIGDMVYGGASKGQSVKNAALK
                     AGIKALSRQALHAAELSFLHPQSGERMVFTAPLPRDLEGLLDLFRSASPGGPGQTGLQ
                     TWKDALR"
     gene            complement(4218..5636)
                     /gene="selA"
                     /locus_tag="PLQ77_06790"
     CDS             complement(4218..5636)
                     /gene="selA"
                     /locus_tag="PLQ77_06790"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPL10419.1"
                     /translation="MDDKRKALLKGLPKIDDVIGLLEKNGIYSKASKDIVREACREIV
                     QRLRDQILSAADKALPSLSFDAASAARAVEERMADLHRFKLRRLVNATGVILHTNLGR
                     APLCAEAVDRIVEVGRGYSNLEFDLIRGERGLRYDHVTGLICALTGAEDALIVNNNAA
                     AVLLTLNTLAEHKEAIVSRGELIEIGGEFRIPDVMTKSNSILREVGTTNRTRLADYEK
                     AIGPNTGVILKVHTSNYRIVGFTEEAELLPLVALGKHHGIPVFDDLGSGCLVDLAEFR
                     LQHEPTVREVLDSGVDVVTFSGDKLLGGPQAGIIVGKKDIVARIKKNPLNRALRIDKF
                     TLAALEATLMRYLAPGDAPRNLRSLRALTEPLGDVKKRARKLMAKLKRAGLSGLTYEL
                     KDSMAAAGGGSLPTEDIPSAVVTVKSAKMSASRMEASLRRAAVPVIVRVDEDEIQIDL
                     RTVAEDEFGFVVDALRGLADSP"
     gene            complement(5798..7057)
                     /locus_tag="PLQ77_06795"
     CDS             complement(5798..7057)
                     /locus_tag="PLQ77_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HPL10420.1"
                     /translation="MINSIVASTAKKQFARALNFQEDDTTGLPACADNQRRLLYVHIP
                     FCEELCPYCSFHRVRLDEPLARRYHAALRREMALYRRQGYRFSGLYVGGGTPTILMDE
                     LAETLQIARDLFPIESVSVETNPNHLTDAHLSVLKRAGVHRLSVGVQTFDDGLLKKMA
                     RYDKYGSGEDIAARLSAAQGVFDTLNADMIFNFPEQTGQMLEADLAVLLKLRMQQITF
                     YPLMVSEFASRRMQKTLGDVNFRREPSFFRLIRRRLEPDYDSSSAWCFSRKKAAQSLI
                     DEYVVDFDEYAGLGSGAIGYLQGMCYANTFDIEKYIQDLSKDRLPIQASRRFDPPDRM
                     RYDFLMKLFSTRMNVEEMEKKYGGRFLKTLWKEMAAFTLAGSFRYFTPRLHLTPRGRY
                     LWVVMMREFFIAVNNFRDHCRREAGLG"
     gene            7263..>7620
                     /locus_tag="PLQ77_06800"
     CDS             7263..>7620
                     /locus_tag="PLQ77_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPL10421.1"
                     /translation="MLRKKSVEMKTPLDAAEKAEIKALVDSFTCRDDASGLAGPQIGV
                     NKRIIIFRNKTGNDQKKKMSPEDYDVLVNPRITQARGEKAAAAEGCLSCPDIQIDVER
                     FPEIKVRALNEKGEKIS"
BASE COUNT         1602 a         2263 c         2150 g         1605 t
ORIGIN      
        1 atccgcgcgt ccgcgacaat gcaggatttg gccgtgcaca tgacggggtt gaggtccacc
       61 gattccacga cgtcccgcat gtccatcatc aaatgagaaa aggcaacgat cgtctttttc
      121 agcgcgttga ggttgacggg ctccgagccg cgatacccgg tcagcagttt gcgggcggca
      181 agctcctgaa tcatgtggtc cgcgtctctg gttttcagag gcgccatccg cagggaaaca
      241 tctttgtaaa tctccacccc gacgcctccc atccccagaa gaatcatggg gccgaactga
      301 aaatcatttt tcgctccgac gatcagttcg agacccttga ccatttcggc aatcagcatt
      361 ccggcaaagc cgtcgatttt agacagacgc gcaagcgtcc tgactagcgt ctcgtcatct
      421 ttgatgccaa ccaccacgcc cttcacatcc gatttgtgga ccacggcagg cgacacaatc
      481 ttggccacca ccgggtagcc gattttccgc gccgccgcaa cggcctcgcc ttcggtcgcg
      541 gcaacgccga atttcggcgt cttgaagcca tagagggaca aaattttctg cgcgtcaggc
      601 tccagcaccc agccccattt tcgggatttt tcaaaaatgg ctttgacttc ctttttcagc
      661 atgactgcct ccttgccaga gcttttgcca tcagcacggc gccttcaata gaagatgacg
      721 ccggaatgcc gttgagctca aagccttcga tcagcatgtt gtatttttcg acgtgcggga
      781 catacgcgat gaacggtttg ccggactgac ggcacacaga gctcaatcga acgccgatgt
      841 ccaggctgac gcccggaatg tagggaagga gcagcaccac gacgcagtcg atttccggcg
      901 aagcggccag cacccgcgcg caggcgacga aatcgtcatc cacggcgctg ccggtgaggt
      961 ccgccgggtt ccccagcgac gcgatggccc gaacgctttc cgtgagctgg tttttgatat
     1021 ccgttttggt ttggtcggaa aattccggaa cggcaagccc cgccatggaa caggcatcca
     1081 ccgccatcgc tccgtgtccg ccgcttccgg tgatgatgcc gatgcggccc tcgatcggcc
     1141 gggtgtagga gctcaggact tcgcaaaagg aaaccatttc gttttcgctt accgcctcaa
     1201 tgatgttatg ctgcgcgacc gcggcggcga aactggcgta gtcaccggcc agcgacgccg
     1261 tgtggctgga tacggcgcgg tttccggcgg ccgttttccc cgatttcatg atcaccaccg
     1321 gtttggagca gacgtccgcc gcctgaacaa actcccttcc ttctccctcg ccgaatccct
     1381 cgatgtaata ggcaatgact ttcgtggccg gatcatgcgc cagataggcc agcagctcct
     1441 tttcccgaat aaaagccttg ttcccgatgc tgacggcctt ggccatgccc acgccctgct
     1501 gggcaaactt gatcatgtgg tccaccagaa ttccgccgct ctggctgacg aatgtcactc
     1561 cgcccgcctc cggcttgatc atgcgttcga tcggcaggaa aaacgtatca acgcggaagg
     1621 gaacatatac gcccaggcag ttggggccga taaaaggcag cccggcctcc ctggccatgt
     1681 ccacgatgcg atcctgcaga tcgccccgtc ctccttcgct gaaccctccg gaaatcacgg
     1741 tggcggattt cacccccgcc gcaatgcagt cggccataac gtccgggacc agctccgccc
     1801 gcgtcgcaat gacggccaga tcgattttgg ccggaatctc cgccacgcgg ggataaaccg
     1861 tttcgttatg cagcgagccg cccctgggat tgaccgcgta aaccttgacg ggatagcgat
     1921 ggttgttttt gtcatagatc acattggcgg gatggcggtc gttgcttgcg gaaacgccga
     1981 tcaccgccat ggtttttggt tcaaacaaag gcttcagatt catgagacct cccttttgtt
     2041 tgcggttttc atcaatggat atggcacaaa tcgtcgttca gcaacagggc tttgcagatc
     2101 gggtctccct tcagcatgag aaaattgatt tgtcgcccgg tgacgccgcc cgagccgcgg
     2161 tgtgaaaaaa aatcgtcctc acggcagacg gtgcacaaac cggatgcgtc aatggaggcg
     2221 tcgggaatgc cgcagtctat gagctgtccg cgattggctt gaaccagatc cagcatccag
     2281 cgatccttgc gctttgacgg aaacaaaaag ttttttgcgt ccggatgaag ccgaaacgcg
     2341 tcagcggcgg gcgcgtccac ttcatagcag cagcggccga tggacgggcc gatcgccgcc
     2401 agcagatcga ccggccggga gccgtattga ttttgcatca ggcgaacgac tttggccgtg
     2461 atgccggcgg cggtcccgcg ccatcccgcg tgcacggcag cgatcacctg attctcggga
     2521 tccgccagaa acaccggcac gcagtccgcc gttttaatgc agatggccag atggacgcgg
     2581 tcggtcacga tggcgtcata attcaggtct tcgcgcgagg taaaatactc gccccgtgac
     2641 gggataacca gaatgccgtc ccggtgcacc tggttgagca ccaggaagtt ctccaccggg
     2701 atgccgaacg cagcagccag cttgttccag ttttgcagca cgcgaaattc ctcgtcgcct
     2761 tcccgaaagc tcatattgag gctctggtag tcatcctcgg aaacgcctcc ctttcgtgcg
     2821 cagaaagcgt gctccaaaaa accacaggcc gcaagaccgg aggattgcag ataggggatg
     2881 ccttttgcgt tcgaccatga aaacattgct tccgtccttt gtctcctctt tttgccagag
     2941 gcgcgggagg aattttgccg ctttttgccg tttatcgtaa agcatccttc caggtttgta
     3001 aacccgtttg tccgggccct cccggcgaag ccgagcggaa cagatccagc aggccctcca
     3061 aatcccgcgg cagtggcgcg gtaaaaacca ttctttcacc gctttgcggg tgcagaaagg
     3121 aaagttccgc ggcatgcagc gcctggcgcg acagcgcttt aatgccggct ttgagcgccg
     3181 cgtttttgac gctttgccct ttggacgccc cgccgtaaac catatcaccg atcacgggat
     3241 agccccgttc ggccagatgc acgcgaatct gatgcgtccg gccggttttg atttcaatat
     3301 ccagaagcgt ggccgtgccg tagcgctcgc gaactttcca cagcgtcagc gcgtctttgc
     3361 catgccggct tttcgtggac atttttttgc ggtctttcgg gtggcgtccc accggcagaa
     3421 caatctcgcc gcttgcgcct ttgacgtctc cccagcaaag cgcgatatat ttcttgtgca
     3481 cgtcatgctt ctcaaactgc gcggagagct tgcggtgcgc ctcgtcggac ttggcggcaa
     3541 cgatcaggcc ggatgtttcc ttgtccagcc ggtggacaat tcccggccgc aaaaagccgc
     3601 cgatgccgga aagatcccgg cagtgaaaaa gaagcgcgtt gacaagggtt tgatccggat
     3661 tgcccggcgc ggggtgcacc accatgcccg ccggtttgtt gacgacaatg atggagccgt
     3721 cttcgtacac aatgttcagg ggaatgtcct gcggggcggc ggcggcctcg accgccggct
     3781 ccagatgcag ctccacccgg tcgcctgcct taagatgctg gctcgccttc ggctcccttc
     3841 cgttcacggt tacattgcct tcttcaacag cgtatttaat ttgcgatcgg gagaaagccg
     3901 gatcggcctg ggagagaaat acatccaggc gctgcccggc ctgcgcggaa tcgacggtaa
     3961 aagacaggtt ttgggattcc tggttttcga tggacacgac attctccagc aaaaagcaaa
     4021 cttgacgaat caggcgtcat gaagacgcag gcggcaactc atcgggctgt tgcccaaata
     4081 tccttttcgc cagggcaaca gcccgtcaac tcctgatatc ccggatttcc gcctgtgcgg
     4141 aaatgacttg gtggatgtgt cttcggaagc ggcctcggcc ctcggattcc cgactgcgcg
     4201 gcaggggccg gcctgcgtca cggggagtcg gcaaggcctc gcagggcgtc caccacaaag
     4261 ccgaattcgt cttcggccac ggtgcgcaga tcgatttgaa tttcgtcttc atccactcgg
     4321 acaatgacgg gcaccgccgc cctgcgcaga ctggcttcca ttcggctggc ggacatcttg
     4381 gcggacttca ccgtgacgac ggccgaggga atgtcctcgg tcggaagcga tccgccgccc
     4441 gcggccgcca tggaatcttt aagctcatac gttaaaccgg aaaggccggc gcgtttgagt
     4501 ttggccatca gtttgcgggc gcgctttttg acgtcgccca gcggctcggt cagcgcccga
     4561 agggagcgca ggttccgggg ggcgtccccg ggcgccagat agcgcatcag ggtcgcctcg
     4621 agagcggcca gcgtgaattt gtcgatgcgc agcgcgcggt tcagcggatt tttcttgatg
     4681 cgcgccacga tgtctttttt gccgacaatg atgcccgcct gcggaccgcc cagaagtttg
     4741 tcgccgctga aggtcaccac atcgacgccc gaatccagca cttcgcgcac ggtcggttcg
     4801 tgctgcagcc tgaactcggc gagatccacc aggcagccgc tgccgaggtc atcaaaaacg
     4861 ggaatcccgt ggtgcttgcc cagcgccacc aggggcagaa gttccgcctc ctcggtaaat
     4921 cccacaatcc ggtaattgct ggtgtgaacc ttcaaaatca cgccggtatt cggaccgatg
     4981 gctttttcat aatcagccag gcgcgtgcgg ttggtggtcc ccacctcgcg cagaatggaa
     5041 ttgcttttgg tcatgacatc cggaatgcgg aattcgccgc cgatttcgat gagctccccg
     5101 cgcgagacaa tcgcttcctt gtgctcggcc agcgtgttga gcgtgagcag aacagccgcc
     5161 gcgttgttgt tgacgatcag ggcgtcctcc gccccggtga gcgcgcagat caggccggtg
     5221 acatgatcgt agcgcaggcc gcgctcgccg cgaatcaaat caaattccag attggagtag
     5281 ccgcgcccga cctccacgat gcggtccacc gcttcggcac agagtggcgc gcgccccaga
     5341 ttggtgtgca gaatgacgcc ggtggcgttg accagacgcc gtaatttgaa gcgatgcaga
     5401 tccgccatcc gctcctccac ggcgcgggcg gccgaggccg cgtcaaagga aagtgacggc
     5461 agggctttgt cagccgcgga caggatctga tcgcgcaggc gctgcacgat ctcccggcag
     5521 gcttcccgga caatgtcctt tgacgccttt gaataaattc cgtttttttc caaaagcccg
     5581 atcacgtcgt caatttttgg aaggcctttg agcaatgctt ttcgtttgtc gtccataact
     5641 cactccagat tccaacgcgc catcatctgc ttaccctata aaatgtcatt tcgagggagc
     5701 aaagcgatcg agaaatccat gtccctatcg tatgcgagga atcttaagat ttctcccttc
     5761 ggtcgaaatg acaaaccact aaaatacatc agcagcacta gcccaatccg gcctcccggc
     5821 ggcagtggtc gcggaaattg ttcaccgcga tgaaaaactc ccgcatcatg accacccaga
     5881 gatagcgtcc gcggggcgtc agatgcaaac ggggcgtaaa gtagcgaaac gatccggcca
     5941 gggtaaacgc ggccatttcc ttccacaggg tttttaaaaa ccgtccgccg tattttttct
     6001 ccatctcctc aacattcatc cgggtgctga aaagcttcat caggaagtcg tagcgcatcc
     6061 ggtcgggcgg atcgaaccgg cgcgaggcct gaatcggcag gcgatccttc gacagatcct
     6121 gaatgtactt ttcgatgtcg aatgtgttgg cgtagcacat gccctgcagg tagccgatgg
     6181 cgccgcttcc caggcccgcg tactcgtcaa agtcaacaac gtactcgtcg ataagcgact
     6241 gggcggcttt cttgcgcgaa aagcaccagg ccgaggatga atcataatcc ggctccaggc
     6301 gccgccggat cagccggaaa aacgacggtt cccgccggaa attcacgtcg cccagcgttt
     6361 tttgcatccg ccggctggca aactcggaaa ccatcaaagg gtaaaacgtg atctgctgca
     6421 ttctcagttt cagcaaaacc gccagatcgg cctccagcat ttggccggtc tgttcgggaa
     6481 agttgaagat catgtcggcg ttgagcgtgt caaatacccc ctgcgcggcg ctgagacgcg
     6541 ccgcgatgtc ttctcccgaa ccatatttgt catagcgcgc cattttcttc agcagaccgt
     6601 cgtcgaacgt ttgcacgccc acggaaagcc gatgaactcc cgcccgtttc agaacggaaa
     6661 gatgggcgtc cgtcaggtgg ttcggattgg tttccaccga aacggattct atgggaaaca
     6721 gatcccgggc aatctgaagc gtttccgcca gttcgtccat cagaatcgtg ggggttccgc
     6781 cgcccacgta caggccggaa aaccggtagc cctgacggcg gtaaagggcc atttccctgc
     6841 gcagggccgc atgatagcgc cgggccagcg gttcgtcgag gcgaacgcgg tgaaaggagc
     6901 agtaggggca aagttcttcg caaaaaggaa tatggacata cagcagacgc cgttggttgt
     6961 cggcgcaggc gggcagcccc gtcgtatcgt cttcttgaaa attcagggcg cgggcaaact
     7021 gttttttcgc cgtactggcg acgatggaat tgatcacaat gcaaggtctt tcgctgtatt
     7081 ccggtacacg ttgtgcttgt ttaacaaaag atggggccaa tttgcaagct gcgtttgctt
     7141 gaaataacga ctgatcggtg ttataggtct tgaaaaatca gcgaaccggt gcaaacaaga
     7201 tggcccccaa aagagttcta accctatgga atgacaacgg tgtcaacgaa acggacactt
     7261 ccgtgctgcg aaaaaagtcg gtggagatga aaacgccgct tgacgccgcg gaaaaagccg
     7321 aaatcaaggc gctcgtcgat tcctttacct gccgggacga tgcttcgggg ctggccggtc
     7381 ctcagatcgg cgtgaataaa cgaatcatca ttttccgcaa taaaactggg aacgaccaaa
     7441 agaaaaaaat gtcgccggag gattacgacg ttctggtcaa tcctcgaatc acccaggcgc
     7501 gcggcgagaa ggcggcggcg gccgaaggat gcctttcctg cccggacatt cagatcgacg
     7561 tggagcggtt tcccgaaatc aaggttcgcg ccttaaacga aaaaggcgaa aaaatcagca
//
LOCUS       DAOPAF010000071         7459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_8954_length_7459_cov_61.150594, whole genome shotgun sequence.
ACCESSION   DAOPAF010000071 DAOPAF010000000
VERSION     DAOPAF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8954_length_7459_cov_61.150594"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLQ77_06805"
     CDS             <1..767
                     /locus_tag="PLQ77_06805"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPL10422.1"
                     /translation="KGEGARILRAARMYAILKGMGYSGVHIGGHNIRYEQVEEIIRQG
                     EMLSPRWTELVRYFHYPIDNGFYFFEANPATGLNRDTPVDRRSRPLEAKVECAYGFSR
                     FFHRLMYEPGKNLYGAMRALCKTVQGTRMEPLFHKLEHFTKVALFDCRDCGDCALPDI
                     AYLCPMSQCPKNQRNGPCGGSFQGWCEIYPKQRLCVHVRAYARLKKHGEESRLAGNIV
                     PPCNWDLYQTSSWINYYLGKDHTSAKQGGTGSPGIP"
     gene            845..921
                     /locus_tag="PLQ77_06810"
     tRNA            845..921
                     /locus_tag="PLQ77_06810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:879..881,aa:Arg,seq:ccg)
     gene            complement(1116..1838)
                     /locus_tag="PLQ77_06815"
     CDS             complement(1116..1838)
                     /locus_tag="PLQ77_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL10423.1"
                     /translation="MKQWTTKGGQVISRLSHGMYNCFLVSCRGRHLLVDTGRKGSWKN
                     LCRELDEQGASGGSLAGLVLTHTHYDHAENAAAIKERYNTKIIVHQKEADYVRKGENP
                     VIHGTLPLTGWISDRLSHLYLSRFFRYRPVDGDLTIADRYDLSPLGFQGYLLATPGHS
                     PGSLSVILENEIAIVGDALFGVFRGSVIPPFAADEKLMAASWGNLLKTGCSVYLPAHG
                     TARDREVLERQYEKYKRNYDRS"
     gene            2293..3570
                     /locus_tag="PLQ77_06820"
     CDS             2293..3570
                     /locus_tag="PLQ77_06820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPL10424.1"
                     /translation="MSDLIFSLSSWPRAIVHIDGDAFFTSCEEAVHPELRGKPLITGG
                     ERGIIACASYAAKRFGVKRGVPLSDARRMCPDLIILPSDYETYSLFSRRMFAVMRRFT
                     PDVEEYSIDEAFSDITGMRRALKASYEDIALRMKTEIERDLGITVSAGLSITKVLAKV
                     ASKYQKPAGMTVIPGRDIARYLHDLPVGSIWGIGPATTNYLAKLGIRTALAFAQLPEA
                     TVRKKLSKPGVEIWQELRGQSVYPVISDEKSSYASISKTKTFSPPTSDADYLFAQLAR
                     NLESACLKARRYDLAPKKILVFLKSQRFETAGSEAALSRPCACPLEVSGVVRELFNAC
                     RRPCELYRATGVILADLVADERIQYSLFDDPVQAEKIRDVYAAADGLAEKFGKHAVHL
                     GASHLMEKMGDGRRETPAARKQAKPAEETKNRR"
     gene            complement(3567..4721)
                     /locus_tag="PLQ77_06825"
     CDS             complement(3567..4721)
                     /locus_tag="PLQ77_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPL10425.1"
                     /translation="MKPERRGVLYEFILFGFFLASKIMRHKAHLMGKENLSRMPTPVM
                     FAVTHDSYFEVPSLSRIYYALKPRPDFMVMVKNDFLSGRYLSTNYGKKNRLLRGILLM
                     IDRLGLPLAIFRKLKVAAIDRPFVESFNRKKEDLRREISGQLNQFKESSARGISTLIF
                     PEGTTWGYGGLKKIHSSVYHILENTFQGTGRKVFILPVNIKVDRLVKGAKDVFIHIGQ
                     PVFFRKPKDEFNRTLFELLQQLHTITFSQVAAYYLKRLAEIKQDAGRTVVFRKELFVR
                     NLEGILLEMSRLARERALPGFDPGLANRRYLADKTEHFTKYCLKMQYLQKKRLSDRKE
                     ALLLNVEKILANYSVREFRKANPLGFCANELASLGEQTVRPIFDARLRFR"
     gene            complement(4772..5392)
                     /locus_tag="PLQ77_06830"
     CDS             complement(4772..5392)
                     /locus_tag="PLQ77_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPL10426.1"
                     /translation="MRFFPKEESFFDFFEELADKIEEGGLFFLEMTRHRNYTAARVSR
                     LKEIEHEADVIAHKTYERMHKTFLTPIDREDIYALVNKMDEILDHIEGTAVRVYMYKV
                     KKPNDDIVKQAEILFSAIQKIKSIIQGLRNMKNSRMILDGCVEINTLENAGDVALRAM
                     ITNLFIHEQDAIELLKWKEIFERIEEAIDVCEDVSNILEGIVLKHA"
     gene            complement(5442..>7459)
                     /locus_tag="PLQ77_06835"
     CDS             complement(5442..>7459)
                     /locus_tag="PLQ77_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10427.1"
                     /translation="VFDANLTIASVSPSAERILGYRPEEFVGRSVEDLKTIMTPQSFQ
                     LAAQNIGEILEGKIITSAVYDVIAGDGTLKYLDISGSPLFRDGRIVGLVCDARDVTER
                     VLAENARRDSEELFGQFMRYFPGSAYIKDSDRRIIYLTGSVEEYFGIKPKEWLGKRSE
                     DIWPEEIALVSKRDDEAVLRGEVVQSVTQRSQKDGPHTWITHRFPIYRQNKPPLIGCI
                     SLDITRQKKAEENFRRSMDDSPLGIRIVTADGETLYANRAILDIYGFENLAEFQSVPL
                     KQRYTQASYEEYLARKAKRDRGERDQDEYTIDIVTKNGDIRHVRAFRKQIIWNGEQHD
                     QIIYQDITRQIQAEKALRESENQYRSVFNNAQEGIFRTTTEGRFLMANPAMAKILGYD
                     SSEELMDSVTDVANQLYAHPEQREQTIKRMEQQALIENDELQFIRKDKSRIWVSRTIQ
                     TIRDDQGKTLYFEGLMEDITSRRNSVEQLRKALGGTIQAIASVVETKDPYTAGHQRRV
                     ADIARAIAREMELSQEQISGLRMAAIIHDIGKVSVPSEILSSPRKLTPLEFSLIKTHA
                     QSGYDIVKDIEFPWPIARIIIEHHERMDGSGYPNGLIGDKILPESRILAVADVVEAMA
                     THRPYRPSLGLDKALEEITQNRGRLYDPEVVDACLRLFREKGYVIRD"
BASE COUNT         1605 a         2154 c         1984 g         1716 t
ORIGIN      
        1 acaaggggga aggcgcgaga atccttcgcg ccgccaggat gtacgccatt ctgaagggaa
       61 tgggatacag cggcgttcac atcggcgggc acaatatccg ctacgaacag gtggaagaaa
      121 tcatccggca gggagaaatg ctctccccgc gatggacaga actggtccgg tattttcatt
      181 accccatcga caacggattt tatttctttg aagcaaatcc ggcaaccgga ctcaaccggg
      241 acacccccgt cgatcggcgc tcccgaccgt tggaggcaaa agttgaatgc gcctatggct
      301 tttcccgatt ttttcaccgg ctgatgtatg aacccggaaa aaacctctac ggcgcgatga
      361 gggcgctttg caaaaccgtg cagggcacgc ggatggagcc cctcttccac aaactggaac
      421 acttcaccaa ggttgctctc tttgactgcc gcgactgcgg cgactgcgcc cttcccgaca
      481 tagcctacct gtgtcccatg tcgcagtgcc cgaaaaacca gcgcaacggc ccctgcggcg
      541 gaagtttcca gggatggtgc gagatttatc ccaagcaaag actttgcgtc catgtccggg
      601 cctacgcccg gctgaaaaaa cacggcgagg aatcacgcct ggccggaaat atcgtgccac
      661 cctgcaactg ggacctttat caaacctcct cctggatcaa ctactacctg ggaaaggatc
      721 acacctccgc aaaacaaggc ggcacggggt cgcccgggat tccctaacgg ttttcccttc
      781 gttttcagga aaatggaatt taaccgttga agattatgaa gggatagtgt agaagcaccc
      841 gcacgggcga ttagctcaga tggatagagc gctggcctcc gaagccatag gccgagagtt
      901 cgagtctctc atcgcccacc aagccatcat ccgacatcct ccggagaagt tcgggaaacc
      961 cgcgcaagcc gcgggttttc ttttgtcggg aaaacaaaac aatgtatttt ccccgtcagg
     1021 tccgcggcag cggtttcgcg ccggcgcggc gcgttcagcc ggcccgaaga gactgaaaaa
     1081 gctggcttcc cccgaactat cgaaaaccgg ttacgctagg accggtcata attccttttg
     1141 tacttttcat actgccgctc gagaacctct ctgtcgcgag ccgtcccgtg cgcgggaaga
     1201 taaacggaac agcccgtctt cagcaggttt ccccagcttg ccgccatgag tttctcgtcc
     1261 gccgcgaacg gaggaatcac cgaaccccgg aaaacgccga acagggcatc gccgacgatc
     1321 gcaatttcat tttcaaggat gacgctgagg gacccgggcg aatggcccgg cgtggcaaga
     1381 agatatccct gaaaccccaa cgggctcaaa tcataccggt ccgcgatcgt cagatcgccg
     1441 tcaacgggcc ggtaccggaa gaaccgtgaa agatacaggt gggacaaccg gtcggagatc
     1501 catccggtca gcggcagcgt tccgtggatc acgggatttt ctcccttgcg cacatagtcc
     1561 gcttccttct ggtggacaat gattttcgtg ttgtagcgct ctttgatcgc cgccgcgttt
     1621 tccgcgtggt cgtaatgggt gtgcgtcagg accagcccgg ccagcgagcc gccgcttgcg
     1681 ccctgctcat ccagttcccg gcacagattc ttccaactgc cttttctgcc ggtgtccacc
     1741 agcagatgcc ggccgcggca tgaaacgaga aagcagttgt acataccgtg ggaaagcctg
     1801 gagatgacct gcccgccttt tgtcgtccat tgtttcatcc cttccccctc cggctgaagt
     1861 tcgcttgtgc cgccgacgtc tgttcccggc cttttgaaac gtggcgcccc ggaattttcc
     1921 ccgtcagcct gcgcccgcgg gaaccgcctg cgttccgcct cgcctgacgt atctaccaca
     1981 aatagaagct gttttcaggt atttttcagc ccggcgcagg ctcacgtccg gtgccggcag
     2041 gcgaatttgc cttgacaata gaacgtgcgt tctatacaga aaatcccggg ggaaatcgaa
     2101 agaaatcccg accctggtga atggtaaaag aagcgacaag tgatgagaga gaaaaagtat
     2161 cgaagggcgc gtcatgacat ctgcactgcc agcggggcta aagccccgca ctacattgtc
     2221 gccgcaccca tcatctattg cccatcgcct attaccgata acccatgacc tattaccctt
     2281 gacctgtttt ttatgtccga tttaattttt tctctttctt cctggccgcg ggccattgtc
     2341 cacattgacg gagacgcttt ctttacgtcc tgtgaggaag cggttcaccc ggagctccgg
     2401 ggaaaaccgc tgattaccgg cggagagcgc gggatcatcg cctgcgcgag ctatgcggcc
     2461 aaaaggttcg gcgtcaagcg cggtgtgccg ctttccgatg cgcgccggat gtgtccggac
     2521 ttaatcatcc tgccgtcgga ttacgaaacc tacagcctct tttcccggcg gatgtttgcc
     2581 gtcatgcgcc gcttcacgcc cgacgtggag gaatattcca tcgacgaggc gttttccgac
     2641 atcaccggca tgcgccgggc gctgaaggcg tcctatgaag acatcgccct gcggatgaaa
     2701 acggaaatcg agcgggacct gggcattacc gtgtcggctg gattgagtat tactaaagtg
     2761 ctggccaagg tagcatccaa atatcagaaa ccggcgggca tgacggtcat tcccgggcgg
     2821 gacatcgccc gctatctgca cgatctgccg gtgggcagca tctgggggat cggcccggcc
     2881 acgacgaact atctggcaaa gctcggcatc cgcacggcgc tggcctttgc gcagcttcct
     2941 gaagcaaccg tgcggaagaa actctccaaa ccgggcgtgg aaatctggca ggagctacgc
     3001 ggccagtcgg tgtatccggt gatatccgac gaaaaaagct cctatgcctc catcagcaag
     3061 accaaaactt tctcgccgcc gacctccgac gccgactatc tttttgcgca gctcgcgcgc
     3121 aatctggagt cggcctgtct caaggcccgg cgctacgatc tcgcgccgaa aaaaattctg
     3181 gtatttctga aaagccagcg tttcgaaacg gccgggagcg aagcggctct gtcgcgcccc
     3241 tgcgcctgcc cgctggaggt ttccggcgtc gtccgggaac tttttaacgc ctgccggcgg
     3301 ccgtgcgagc tgtaccgggc gacgggtgta attcttgcgg atctcgttgc cgatgagcgc
     3361 attcagtatt cgctcttcga cgatccggtg caggcggaaa aaatccgcga cgtctatgcc
     3421 gcggccgacg gactggcgga gaaattcggc aaacacgcag tgcacctggg cgcatcacac
     3481 ctgatggaaa agatgggcga cgggaggagg gagacgcccg ctgcccgaaa gcaggcgaag
     3541 ccggcggaag aaacaaaaaa ccgtcgttaa cggaaacgga gacgagcgtc aaaaatgggg
     3601 cggacggtct gttcgcccag cgacgccaac tcattggcgc agaatcccag aggattggcc
     3661 ttgcgaaatt cccggacgga gtagttcgcc aggatttttt caacgttgag aagcagcgcc
     3721 tctttccggt cgctcaaacg ctttttctgc agatactgca tcttcaggca gtattttgta
     3781 aaatgctccg ttttgtcggc aagataccgg cggttggcga ggccgggatc aaagccgggc
     3841 agcgcccgtt cgcgcgccag ccggctcatc tccaggagaa tcccttccag attccgcaca
     3901 aagagttctt tgcgaaaaac gaccgttcgg ccggcgtcct gcttgatttc cgcaaggcgc
     3961 ttgaggtaat aggcggccac ctgactgaag gtgatggtgt gaagctgctg gaggagctca
     4021 aagagcgtcc ggttgaattc atccttgggt ttgcgaaaaa acaccggctg accgatgtgg
     4081 atgaaaacgt ctttcgcccc cttcaccaga cggtccacct tgatgttgac gggcagaata
     4141 aacactttcc gcccggtgcc ctggaacgtg ttttccagga tgtgatacac ggagctgtga
     4201 atttttttca ggccgccgta tccccaggtc gttccttcgg ggaaaatcag cgtcgatatc
     4261 ccccgcgcgc tgctttcctt gaattgattc aactggccgg atatttcccg ccgcagatcc
     4321 tcttttttcc ggttgaaaga ctccacaaac ggacggtcga tggccgccac tttcagcttg
     4381 cggaaaatcg ccaggggaag ccccagccgg tcgatcatca gcagaatgcc tctcaaaagc
     4441 ctgttctttt tcccgtagtt tgtggaaaga taccggccgc tcagaaaatc gtttttgacc
     4501 atgaccatga aatccggcct gggtttcagg gcatagtaaa tccgcgacag ggacggaact
     4561 tcaaaatagc tgtcgtgggt cacggcaaac atgaccgggg tgggcatccg ggaaaggttc
     4621 tccttcccca tcagatgggc cttgtggcgc atgattttgg aagccagaaa aaatccgaac
     4681 aaaatgaatt catacagcac gccgcggcgc tccggtttca tgttcgcgct cctttcgccc
     4741 cgaaagccct gaaaaacggc aagggattct gtcaggcatg tttcaggaca atcccttcca
     4801 gaatattgga gacgtcttcg cagacgtcga tcgcctcttc gattcgttca aaaatctctt
     4861 tccacttcag cagttcgatc gcatcctgct cgtggataaa caggttggtg atcatggccc
     4921 gcagcgcaac atcgccggcg ttttccagcg tgttgatttc gacgcagcca tccagaatca
     4981 tccggctgtt tttcatgttg cgcaacccct ggatgatgct tttgatcttc tggatcgccg
     5041 aaaaaagaat ttccgcctgt ttcacgatgt cgtcattggg cttttttacc ttgtacatgt
     5101 aaacgcggac ggcggtgcct tcgatgtggt ccagaatttc atccatttta ttgaccagcg
     5161 cgtagatatc ttcccggtcg atgggcgtca gaaaagtttt gtgcatccgt tcgtaggttt
     5221 tatgagcgat gacatccgcc tcatgctcga tttccttcag ccgggagacc cgcgccgccg
     5281 tataattccg atggcgggtc atttccagga aaaacaatcc gccctcttca atcttgtcgg
     5341 ccagttcttc aaaaaaatca aaaaaacttt cttctttcgg aaagaaccgc atatcaaaag
     5401 ctctccttta atttttttga gcaacgcttg caacgacacc gtcaatcccg gatgacatag
     5461 cccttttccc ggaaaaggcg caggcaggcg tccacgacct ccggatcata aagccggccc
     5521 cggttttggg taatttcttc cagcgcttta tcgagtccca gcgaaggccg gtagggacga
     5581 tgcgttgcca tagcttccac aacgtcggcc acggccagaa tccgcgactc cggaagaatt
     5641 ttgtctccga tcagaccgtt gggatacccg gagccgtcca tccgctcgtg atgctcaata
     5701 atgatccgcg caatgggcca gggaaattca atgtccttga cgatgtcgta accggattgc
     5761 gcgtgcgttt tgatcagact gaattccagg ggcgtcaatt tcctcggcga actgaggatc
     5821 tcggaaggta cggagacttt cccgatgtcg tggatgatgg cggccatccg caagccgctg
     5881 atctgctcct gggacagttc catttcccgg gcgatggccc gggcaatatc ggcgacacgg
     5941 cgctgatggc ccgccgtgta agggtctttg gtctccacga cggaggcaat ggcctgaatg
     6001 gtccctccca gggctttccg caactgctcg acgctgtttc gccggctggt gatgtcttcc
     6061 atcagccctt caaaatacag cgttttcccc tgatcatcgc gaatggtctg aatggtgcgg
     6121 gatacccaga tgcggctttt gtcttttctg atgaattgca gctcatcgtt ttcgatcagg
     6181 gcctgttgtt ccatccgctt gatggtttgt tccctttgtt cgggatgagc atacaattgg
     6241 ttggcaacat cggtcacgct gtccatcagt tcttcggaag aatcatatcc cagaattttc
     6301 gccatggccg gattggccat aagaaatctt ccttcagtcg ttgtccggaa aatcccttcc
     6361 tgggcattat taaaaacact gcggtattga ttttcgctct cccgcagcgc cttctcggcc
     6421 tggatctgcc gggtaatgtc ctgatagatg atctggtcat gctgctcccc gttccagata
     6481 atctgcttgc ggaaagcccg gacgtggcgg atgtcgccgt tcttggttac gatatcaatc
     6541 gtatattcgt cctgatctct ctctcccctg tctctttttg cctttctggc cagatattct
     6601 tcatagctcg cctgggtata acgctgtttc aggggaacgc tctgaaactc ggccaggttt
     6661 tcaaacccgt aaatatccag aatcgcccgg ttggcataaa gcgtctcgcc gtccgcggta
     6721 acgattcgga tccccagcgg agaatcatcc atggagcgac ggaaattttc ctctgctttt
     6781 ttctgcctgg tgatgtccag ggatatacac ccgatcaggg gcggcttatt ctgccggtaa
     6841 atgggaaacc ggtgcgtgat ccaggtatgc ggtccgtctt tctgggacct ttgcgtgacg
     6901 gactgaacca cctcgccccg caaaaccgct tcatcatctc ttttggagac cagagcgatc
     6961 tcttctggcc agatgtcctc gctccttttt cccagccact ccttcggttt tataccgaaa
     7021 tattcttcaa cactacccgt caaataaatg atgcgccggt ctgaatcctt gatgtaggcg
     7081 ctgcccggga aataccgcat gaactgccca aaaagctcct cgctgtcccg ccgggcgttc
     7141 tctgcgagca ctcgctccgt gacgtcccgg gcatcgcaaa ccaggcctac aatcctgccg
     7201 tcgcgaaaaa ggggcgatcc gctgatatcc agatatttca gcgttccatc tccggcaatc
     7261 acatcgtaaa cagccgatgt gatgatttta ccctccagaa tctcaccgat gttttgggcg
     7321 gccagttgga aagattgcgg cgtcatgatg gtctttaggt cttccacgga ccggccgaca
     7381 aactcttccg gacggtatcc cagaatcctt tccgcgctcg gcgagacact tgcaatggtg
     7441 agatttgcgt caaaaacaa
//
LOCUS       DAOPAF010000072         7418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9015_length_7418_cov_34.332202, whole genome shotgun sequence.
ACCESSION   DAOPAF010000072 DAOPAF010000000
VERSION     DAOPAF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9015_length_7418_cov_34.332202"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..430
                     /locus_tag="PLQ77_06840"
     CDS             104..430
                     /locus_tag="PLQ77_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPL10428.1"
                     /translation="MRQFVEFQQNIKKYEAGKVKILGLSRDSAESHRKFIAKHGLHNM
                     TLLVDEKGKIAKRYDADHWILPLSKRVYLIVDQNLNILYRKDMGFGLLPDQTQTLMME
                     IDRLIK"
     gene            complement(512..>1348)
                     /locus_tag="PLQ77_06845"
     CDS             complement(512..>1348)
                     /locus_tag="PLQ77_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPL10429.1"
                     /translation="IVPGVCKGELSVSVCMTEPEAGSDLTSLSTKAVDDGDHYILNGR
                     KVFITGGGHASHYFVYTRFGDAPGYKGIGGLLVEKGTQGFTFGKQEDFLGLRGMPSCE
                     LIFEDVRVPKKNLVIKAGDFNKLMLTFDIERCGNAAMCLGVAGAALNESIAYAQARQA
                     FGRPICEFQAIQFMTVDMAMKLDAARLLVYRAAAGAGQGLPSIYEASMAKCFANEMVI
                     EVTNTAMQVFGGYGYSKEFPVERMLRDARAWGVAGGTVQMLRLTMASVLYGRRFDQRR
                     GK"
     assembly_gap    1349..1448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1572..2372)
                     /locus_tag="PLQ77_06850"
     CDS             complement(1572..2372)
                     /locus_tag="PLQ77_06850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10430.1"
                     /translation="MIEHPKDYIKPELMACCGAREIHDGDVVVVGTGFPAMSANIAKH
                     THAPGAVMMQESGVYDARPGRPALSVGDACLNPGSAMIGGLTDVMGMFLQGGWVDVGF
                     LSGAQVDRYGNLNTTCIGPYDSPRSRLPGSGGANPIGALARRTLIIVQHDPRRLAEKV
                     DFITTPGYLNGPGARERWGLPAATGPWVIITNKGVMRFDAETKEAYLESYHPGCTLEE
                     IRKNTGWDLKISAEVHETEPPRAVELKAIREVLDPHRMIRVYEGKGYV"
     gene            complement(2387..3322)
                     /locus_tag="PLQ77_06855"
     CDS             complement(2387..3322)
                     /locus_tag="PLQ77_06855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPL10431.1"
                     /translation="MNKVMTMQKAIAEHVHDGDFLYIGGYICRTPFSAVHELIRQGKK
                     ELTITRGNASDDFDMLIGAGAVKRFISTFISLGVYGLARCYRRSLEKGIPHKIEVEEY
                     TNLSLPMMLMAGAMGMPFIPVKDMVGTDLMNVKGFMGEDKYRMISSPFDGSPVLLVPA
                     LKPDVGIIHVQQADEEGNAQMWGIGGDCKYGANASKKVIVSCERIVSRDVIGKDPSRT
                     IVPDFKVVAVVEEPFASHPGYTPGFYDLDFSFGYLYQQASNTEEGFRAFLQEWIMDIP
                     DRASYIQHYIDRFGYAQFRKLQAKLDYGFPVSYAY"
     gene            complement(3402..4829)
                     /locus_tag="PLQ77_06860"
     CDS             complement(3402..4829)
                     /locus_tag="PLQ77_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPL10432.1"
                     /translation="MKTYDKIYINGQWVSPAGKDKVKVINPCTEEAIASVPLGNEEDV
                     QKAVIAARQAFPSWSKTSVADRIKLISKLSEAIAARQNEIGDTISREMGMPSAWSRMI
                     QVGLPVATLNSFVPILENYFFSYPMGRTEIVKEPIGVCGFITPWNYPLHQLVGKVAPA
                     LAAGCTMVAKPSQLAPLNAYAFTEIMQEIGVPPGVFNLVPGAGSKVGYALSSHPDVDM
                     VSLTGSTKSGIAVAQAAATTIKRVTLELGGKSPNVLLEDADIPMAVTKGVYNCFLNSG
                     QTCIALSRMIVPAHKQKEIVEMAKAIVESMVMGDAFTDGVYLGPMVDDAQRQSVREYI
                     RAGIREGATLVTGGPETPENLPKGFFVKPTLFADVKPDMIIAQEEIFGPVLCIIPYRT
                     EEEAIAIANNSVYGLSGSVWSADSEKARAAARQIRSGQVFINGADFDIDAPAGGYKQS
                     GLGRERSKYGLEEYLEIKALIAQNP"
     gene            complement(4826..6016)
                     /locus_tag="PLQ77_06865"
     CDS             complement(4826..6016)
                     /locus_tag="PLQ77_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL10433.1"
                     /translation="MEYRFELPTKMIIGEGCSKQLGENVKLTGAKHVLCVFDKGVEGA
                     GLVAPLIADMETAGLKVTPFNGVLPDPPMEVIDQCTQLAREAKPDVFVAVGGGSSIDT
                     AKAVNANLSNPGTLRDHAINLNGLKVFPFENPLKPFFALPTTAGTGSEVSPSAVVTDH
                     EAKLKVSVMAPDLVPTMAIIDPALTVGLPVNVTASTGLDAFAHAMEGLMGGLAIFTPS
                     PMRDSFAFTAIELVLESLLPAMKNGKDIKARTDMSYAAFMSILGAGGGLSMGHCLGHA
                     MGEVCGIHNHGFLCASILPYNIEFMAELIPQKLNKLASLFKIDPNGKSVAQIGTAIKL
                     AIQAFYKDCGIPPLKDMGLNLSDTEEIIHHTTSGTWYLLASKKPSEEELTRWIQAVYE
                     GGNA"
     gene            6541..>7418
                     /locus_tag="PLQ77_06870"
     CDS             6541..>7418
                     /locus_tag="PLQ77_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPL10434.1"
                     /translation="MNESLIFQETPDLVNPYLFIGYRGWLNAGELGTGSIEYLRRKLN
                     ARKFAAIDPGHFYIGQVPGFDSAQIMRPQTVVEGGLIKSLEEPANEFFYWQSGGENDL
                     ILFTGFEPNVAWPEYTSAILSMAKRCNVCRIYTLGGVFDQVPHTRETSVYAVLSRPEM
                     KREFQSFPLLNYNGPCSFSTLLVNHAGRAGIEAASIVARVPPYIQTFHEKIACDLLQK
                     VFAHTSLHVDLSDLRKIGNTVAELMDNDFRQNKTAMEQLRKLEEMYDAALIQGYGYKE
                     VNVDELMQEMLDTKMSG"
BASE COUNT         1700 a         2044 c         1904 g         1670 t
ORIGIN      
        1 gctgccggat caggacggaa aaatgtggaa gctcagcgat gcggtgaaag aataccgcgc
       61 cgtggtgctg gccttttatc ccaaagacga taccggggtc tgaattcgcc agtttgttga
      121 gtttcaacaa aacatcaaaa aatacgaagc cggaaaagtg aaaatattgg gcctgtcgcg
      181 ggattcggct gaatcacacc ggaaattcat cgccaaacat ggactgcaca acatgaccct
      241 gctggtcgat gaaaagggca aaatcgccaa aaggtacgac gcggatcact ggatattgcc
      301 cctgtccaaa cgggtgtatc tgatcgtcga tcagaatttg aatattctgt accggaaaga
      361 catgggcttt ggcctgctgc cggatcagac acaaacgctg atgatggaaa tcgatcgttt
      421 gataaagtaa aaaaatattt cagaaccgga caacggagcg gttcgaaaaa aaacgtgaaa
      481 taccgttgcc cggttccgga aatcctaccc tctacttccc gcggcgctga tcaaaccgac
      541 ggccatacag aacgctggcc atggtcaaac gaagcatctg cacggttccg ccggccacgc
      601 cccacgcccg ggcgtcacgg agcatccgct ccaccgggaa ttccttgctg tagccgtagc
      661 ctccgaagac ctgcatggcc gtgttggtca cctcaatcac catttcgttg gcaaagcact
      721 tcgccatgga agcctcgtaa atggaaggca gtccctgccc cgcacccgcg gcggcccggt
      781 aaacgagaag tcgcgccgca tccagcttca tggccatgtc cacggtcata aactggatcg
      841 cctggaattc gcagatcggc cggccgaagg cctgacgggc ctgggcatag gcgatggact
      901 cgttgagggc ggctccggca acgcccaggc acatggccgc attgccgcac cgctcgatgt
      961 cgaaggtcag catcagttta ttgaagtcac cggctttgat gaccaggttt ttcttgggaa
     1021 cgcgaacatc ctcaaagatc agctcacagg agggcatccc ccgaaggccg agaaaatctt
     1081 cctgcttgcc aaaggtaaag ccctgcgttc ccttttcaac cagcagaccg ccgattccct
     1141 tgtagccggg agcgtctccg aaacgggtat atacaaaata atggcttgcg tgtccgccgc
     1201 ccgtgataaa gacctttcgg ccgttgagga tgtaatggtc gccgtcatca acggcctttg
     1261 tgctcaaaga ggtcaggtcc gaaccggctt ccggctccgt catgcagacg gatacgctga
     1321 gctctccctt gcacacccct ggaacaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnncc catggtctga agcgttcgaa tcaacattct ttgttcttca ttcaatgaaa
     1501 aatccattta ccattctcct tttatcttga tcgctccatc agcccgatga atgctctgac
     1561 atcagagcag tttaaacata ccccttgcct tcataaaccc gaatcatgcg gtgcggatcg
     1621 agaacttccc gaatcgcctt cagctccacg gccctgggcg gttccgtctc gtggacctcc
     1681 gcggagattt tcaaatccca gccggtattt ttccttattt cctccagggt gcagcccgga
     1741 tgatagcttt ccagataagc ttccttggtt tccgcgtcga agcgcatgac acctttattg
     1801 gtaataatga cccatggacc ggttgcggca ggcagtcccc atcgttcgcg ggcgccgggt
     1861 ccgttgagat agccgggggt ggtaataaag tcgacttttt cggcaagacg ccggggatca
     1921 tgctggacga tgatcagtgt tcgcctggcc agagccccga tgggattggc cccgccgctt
     1981 cccggcagcc tgctgcgcgg ggaatcatag ggaccgatgc aggtcgtgtt cagatttcca
     2041 tagcgatcca cctgagcgcc ggacagaaat cccacgtcca cccaaccgcc ctgcagaaac
     2101 attcccatca catcggtcag cccgccgatc atggccgacc cgggattcag gcacgcgtcg
     2161 ccgacggata aggccggacg tcccggtctg gcgtcataaa cccccgattc ctgcatcatc
     2221 accgccccgg gggcatgggt atgcttggcg atgttggccg acatggcggg aaatcccgta
     2281 ccgacgacaa ccacatcacc gtcatggatt tctctggcgc cgcaacaggc catcagttcg
     2341 ggcttgatat agtccttcgg atgttcaatc atttgtattc acctcctcag taagcgtaac
     2401 tgaccggaaa accataatcg agtttcgcct ggagtttccg gaactgggca tagccgaagc
     2461 gatcgatgta gtgctggata taggaagccc ggtcgggaat atccatgatc cactcctgaa
     2521 gaaacgcccg aaagccttct tccgtattgg aggcctgctg atacagataa ccaaaactga
     2581 aatccagatc atagaatccg ggcgtatagc ccggatgcga cgcgaaaggc tcttccacaa
     2641 cggcaaccac cttgaaatcc ggcacgatcg tccgggaagg gtccttcccg atgacgtccc
     2701 ggctgacgat gcgttcgcag gacacaatga ccttcttcga cgcattggcg ccgtatttgc
     2761 aatctccgcc gattccccac atctgcgcgt tgccttcctc atccgcctgc tggacgtgaa
     2821 tgattcccac atccggcttg agagccggca cgagcagaac gggggatccg tcaaagggag
     2881 aggagatcat ccggtatttg tcctctccca taaaaccctt cacgttcata aggtccgtcc
     2941 ccaccatgtc tttgacggga ataaagggca tgcccatcgc tcccgccatc agcatcatgg
     3001 gcagcgacaa attggtatat tcctccacct caatcttgtg gggaatgcct ttttccagcg
     3061 aacgccggta gcagcgggcc aggccgtaaa cgccgagaga aatgaacgtg gaaataaatc
     3121 gcttcaccgc ccccgccccg atcagcatat cgaaatcatc cgaggcgttg ccgcgggtaa
     3181 tagtcagttc ttttttcccc tggcggatca gttcgtgaac cgccgaaaaa ggcgtccggc
     3241 agatatagcc gccgatatat agaaaatcgc cgtcatggac atgttcggca attgcttttt
     3301 gcatggtcat aaccttgttc atgaaggtcc tccgttggtt ttgggttgtc cggccgggat
     3361 atgccttccc ggccggaccg gatcttttca ttgaaaaacg attaagggtt ttgtgcgatc
     3421 agggccttga tttccagata ttcttccaga ccgtatttgc tgcgttcgcg tccgagtccg
     3481 gactgtttat accctccggc cggagcatcg atgtcaaagt cggccccgtt gatgaagacc
     3541 tggccgcttc gaatctgacg cgccgcggct ctggcttttt cagaatccgc cgaccagacg
     3601 gagccggaaa gcccgtaaac gctgttgttc gcgatggcga tggcttcttc ttcggtccgg
     3661 tagggaatga tgcacaaaac gggaccgaat atttcctcct gcgcaatgat catatcgggt
     3721 ttgacgtcgg caaagagcgt cggcttgaca aaaaatccct tcggaaggtt ttcgggggtt
     3781 tccggcccgc cggtgaccag cgtggcgcct tcgcggattc cggctcgaat atattcgcga
     3841 acggattgac gctgggcgtc atccaccatc ggccccagat agacgccgtc cgtaaaggca
     3901 tcccccatca ccatggattc gacgatggcc tttgccattt cgacgatttc tttctgtttg
     3961 tgcgccggga cgatcatgcg ggacagggca atgcaggttt gcccggaatt caaaaagcag
     4021 ttgtacaccc ctttcgtcac cgccatggga atatccgcgt cttcgagcag gacattgggg
     4081 gacttcccgc ccagttccag cgtaacccgc ttgatcgttg tggcggcggc ctgggccacg
     4141 gcaatgccgg acttcgtgga acccgtgaga gacaccatat ccacatcggg atgagacgac
     4201 agggcgtagc ctaccttcga tccggccccg gggaccagat tgaaaactcc gggaggcacg
     4261 ccgatttcct gcatgatctc cgtaaatgca taggcattca ggggagccag ttggctgggt
     4321 tttgccacca tggtgcagcc cgccgccagc gccggcgcca ctttgcccac aagctggtga
     4381 aggggataat tccagggcgt aatgaaaccg cagacgccga tcggttcttt tacaatttcc
     4441 gttcgaccca tcggatacga aaaaaaatag ttttccagaa tcggaacgaa gctgttgagc
     4501 gtggcaacgg gcaggccgac ctgaatcatt cgggaccatg cggaaggcat gcccatctcg
     4561 cgggaaatcg tatcgccgat ctcgttctgg cgggcggcga tggcttcgga aagttttgaa
     4621 atcagcttga tccgatcggc aacactggtt ttggaccagg aaggaaaagc ctgccgcgct
     4681 gcgatgacgg ccttctgaac gtcttcttcg ttgcccagcg gcaccgaggc aatcgcttct
     4741 tccgtacagg ggttgatcac tttgactttg tctttgccgg ccggggaaac ccactggccg
     4801 ttgatgtaga ttttatcgta tgtcttcatg cgtttcctcc ttcgtaaacg gcctgaatcc
     4861 agcgggtcag ttcctcttcc gatggttttt tggatgccag aagataccag gtgcccgatg
     4921 tcgtgtggtg aatgatctcc tccgtgtcgg aaagattcag ccccatatct ttcagcggcg
     4981 gaatgccgca atccttgtaa aaggcttgaa tggccaactt gattgccgtc ccgatctggg
     5041 cgacggattt tccgttcgga tcgatcttga acagggaggc cagtttattg agtttctggg
     5101 gaatgagttc ggccatgaac tcgatgttgt aaggaagaat ggaagcgcat aaaaagccat
     5161 gattatgaat gccacagact tctcccatgg catgacccag gcaatgaccc atgctcagtc
     5221 cgccgccggc tcccagaatg gacataaaag cggcatagga catatccgtc cttgccttga
     5281 tgtctttccc gttcttcatc gccggcagaa gactctccag caccagttcg atggcggtaa
     5341 aggcaaaaga atcccgcatg ggcgacggag tgaaaatcgc caaaccgccc atgagtcctt
     5401 ccatggcatg agcaaaggcg tccaatccgg tggaagcggt cacattcacc ggcaagccga
     5461 cggtcagagc cggatcgatg atggccatgg tgggcaccag atcgggcgcc atgaccgaaa
     5521 ccttcagctt cgcttcgtga tcggtaacga ccgccgacgg ggatacttca ctgccggttc
     5581 cggcggttgt gggcagggca aaaaaaggtt tgagcggatt ttcaaaagga aacaccttga
     5641 ggccgttgag gttgatggca tggtcgcgaa gcgttccggg attggacaga ttcgcgttga
     5701 cggctttggc tgtgtcgatg gagctgccgc cgcccacggc gacaaagacg tcaggcttgg
     5761 cctcccgcgc cagttgggtg cactgatcga tcacttccat gggcggatcc ggcagcacac
     5821 cgttgaaagg cgttaccttc aggcccgccg tttccatgtc cgcgatgagc ggcgcgacaa
     5881 gacccgcccc ttccacacct ttatcaaaaa cacacaggac gtgttttgca ccggtcagct
     5941 tgacattttc ccccaactgc ttggaacatc cttctccgat gatcattttt gtaggcaatt
     6001 caaatcgata ttccatgatt cctccttttt agtattcgtt gactacattt caaatgcatc
     6061 ttctccacat ccatcgttgc ttttgtcatt taaaatgctg accattatcc gtcgcgcatt
     6121 ctattcttcg gtacgcaccc tcctttcttc acgaggcatg gtttcacctc aacaaacggc
     6181 gtcatttacg gacaactttt cctcaatcat tcaggcatcg actatgtaga ttgtatatat
     6241 attttattta tatactgtca agcatttttt ctcccatcca gtcaagattc aactatttaa
     6301 caaaattgat gtttttcctt tgacgactgt ccccggtcaa aaaaaataaa tatcggaacc
     6361 gaagaaaatt cttccggctg ataccattca acttgaagca aaaacccatc atcctgaaac
     6421 aaaatacttg actgcaaaaa acgcaataac cataatcaac aagcggtttt ggttttagaa
     6481 aaatcatgcc ggcggtgcgc aatgatgacc gcagccgcga ataacttcca aggagagttc
     6541 atgaacgaaa gtctcatttt tcaggaaaca cccgacctgg tcaatcccta tctgttcatc
     6601 ggctatcgcg gctggctgaa tgccggcgag ctcggcaccg gaagcatcga atatttgcgc
     6661 cgcaagctca acgcccgaaa attcgccgcc atcgatcccg ggcatttcta tatcggacag
     6721 gtcccgggct ttgattcggc gcagatcatg agaccccaga ccgtcgttga aggaggcctg
     6781 atcaagtccc tcgaagaacc ggccaatgaa tttttttact ggcaaagcgg cggcgaaaac
     6841 gacctgatcc tttttacagg ttttgagccg aacgttgcct ggccggagta cacgtcggct
     6901 attttgagca tggccaaacg ctgcaacgtt tgccgcatct atacgctggg cggcgttttc
     6961 gaccaggttc cccacacgcg ggaaacaagc gtctatgccg tgctgagccg tcccgagatg
     7021 aagcgggagt ttcaatcgtt tcctctcctg aactacaacg gaccgtgcag cttctccacg
     7081 ctgcttgtga atcatgccgg ccgggcggga attgaggcgg ccagcatcgt ggccagagtc
     7141 ccgccgtata ttcagacctt tcatgaaaaa atagcctgcg accttctgca gaaagtgttc
     7201 gcccacacat cccttcatgt cgatttaagc gatttgagaa aaatcggaaa taccgtggct
     7261 gagctgatgg acaatgactt ccggcaaaat aaaaccgcta tggaacaatt acggaagctg
     7321 gaagaaatgt atgacgcggc gcttattcag ggttatggtt acaaggaagt caacgtcgat
     7381 gagctgatgc aggaaatgct ggatacgaaa atgagcgg
//
LOCUS       DAOPAF010000073         7385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9069_length_7385_cov_38.685948, whole genome shotgun sequence.
ACCESSION   DAOPAF010000073 DAOPAF010000000
VERSION     DAOPAF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9069_length_7385_cov_38.685948"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLQ77_06875"
     CDS             <1..809
                     /locus_tag="PLQ77_06875"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPL10435.1"
                     /translation="PTLRGKSVINLFYEASTRTRTSFEIAGKRLSADTINIAASTSSA
                     VKGETLLDTARTLEAMNPDIIVLRHSVAGTPHMLARMVKQSIINAGDGAHEHPSQALL
                     DMMTIREKKGRIAGLKVAIVGDILHSRVARSNIYGLSRMGARVVVAGPATMMPRGIER
                     MGVEAFTTIEDAIAGADVVMMLRIQLERQKQNIFPSLREYSHHYCLSRRHMKLTAPDA
                     IVMHPGPINRGVEISPDIADDPACSVILDQVNHGVAVRMAMLYLLSGGNE"
     gene            806..2095
                     /locus_tag="PLQ77_06880"
     CDS             806..2095
                     /locus_tag="PLQ77_06880"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPL10436.1"
                     /translation="MKLLLKGARVIDPAQNLDALMDVLVEGGKIAACKPGIKAPAGAK
                     VLELSGMLVTPGLIDMHTHLREPGYEYRETIASGSAAAVAGGFTSIACMPNTLPVNDN
                     RSVTEFILRKAAEANLANIYPIGAISMGSEGKQMTEFWDLREAGIAALSDDGNPVMDA
                     ALMRRAMEYAYSLELPVIQHCEDKNLSAGGLMNEGYQATVLGLPGIPAIAEEVMVARD
                     ILIAEYTGTRIHFAHVSTTGSVRLIRAAKKRRLKVTAETAPHYFTLTDESLRNYDTNY
                     KVSPPLRGDADVSAIKEGLADGTLDAIACDHAPHGRTDKEVEFEYAACGISGLETSLG
                     LSLSLVQEGILPWPELIAKMSCNPARILNLPKGTLAKGADADITVIHPDLAWSVDVAA
                     FHSLGKNSPFSGRKLKGRAVLTIVGGDIKYDGRASQP"
     gene            2092..2865
                     /gene="recO"
                     /locus_tag="PLQ77_06885"
     CDS             2092..2865
                     /gene="recO"
                     /locus_tag="PLQ77_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPL10437.1"
                     /translation="MSARETHKTAGFVLRSLQYGESDLIVTFYTREFGKIKGIAKGAK
                     RSRKRFANVFEPFSLTQIIFSRRNRDSLAFIDACDILDHFAAIRMDLEKTLTASYFID
                     LAEHFSPEGKRNDGVFALLLDFLPLLTRENVSEPIIRFFEMRLLKAAGFEPELAACVR
                     CKTPVANGSPYYFFPKEGGIFCTACARPQRYDQSISAGAVRTLLLGKDMDLEKMKMIS
                     MTESLASESRSILCGFISYVLGREVQSLKVLEQVRRYCP"
     gene            2879..4138
                     /locus_tag="PLQ77_06890"
     CDS             2879..4138
                     /locus_tag="PLQ77_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HPL10438.1"
                     /translation="MRRPELLAPAGNLEKLRTALLYGADAVYAGVPGLSLRGAPAEMS
                     LEDLASGVSEAHAQGIRVYAAVNTFARNADLKQARLHLPDVAATGVDALIVSDPGLIR
                     LARRTVPQIPLHLSTQANTTNADAVRFWRDLGVSRIILARELPLGEVEEIASALPDVE
                     LEIFIHGAMCMAYSGRCYLSAWRNRRSANEGDCTQPCRWEYRLSESTRPEDPIVVEED
                     ERFSYLLSSKDLCLLEHLPEVLATGVAGVKIEGRMKSAYYAAVVTRTYRQALDALCRD
                     KEAYRCDPRWIEELKTVSHRGYTTGFAFAEEKINESSPDVKNIQTHEPAGMVVGYDGI
                     RKQMRMEVRNFLQAGEEMELLLMEETLPVSAQPMTDDRGSPIDRAHAGNLIRVPLPFP
                     APTGALLRKKTVGAKTEETKRKPSENG"
     gene            complement(4147..4776)
                     /locus_tag="PLQ77_06895"
     CDS             complement(4147..4776)
                     /locus_tag="PLQ77_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10439.1"
                     /translation="MKRIHLVLYFFLIVFLLAGCAKGPSVTKKEEPKLKETPPAPVVV
                     KKPPVAPPPPAPAIEKLPEIARSIPDSKVWVRYGKTKMGENYYNKTNITRSSDVATVN
                     VYRVVTDSCRKQMVEEVRKYNPKKSIKYHYYEHDVRVDEIDCRNQKWRVKELIHYDEK
                     GKVLDHHTYENEPWEKIKFVTDHHTLQKSICVAEEKPAAKKPAAKKKKK"
     gene            complement(5045..6217)
                     /locus_tag="PLQ77_06900"
     CDS             complement(5045..6217)
                     /locus_tag="PLQ77_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPL10440.1"
                     /translation="MEYGRVGLIKQSSSAIQNTFILRRKIMKKFASLVVVLLVMLLVS
                     TGNAQVRSGSFTVAPTLGMYVFEGNEDMDRAMPLVGMRLGYNFNKYLGVEGFVHYAKT
                     DNNAWSPKQNVDFTGYGAEVIFNLLPNQALVPFLAAGVGGVHYSYGVFEDDSDYGTDN
                     YKKDKLAFDYGYGLKWFLSDWFALRLDIRHVLPIEKLHNNMLVSLGLNFAFGGAKKAV
                     ATPVSAAEPVMEEAKPQAEAAPVVEEAKPAVAAAAPVVTEEVVRPQPRTQVREEVDEM
                     GRATLEVLFDFDKATIKKNYTKDIDHMVGVMKEHSDVNLTIEGHTDNVGKAEYNKNLS
                     QKRAEAVKNYMVEKGGIDAQRLNAVGYGQEKPVASNKTKAGRAKNRRVEAAVDYKK"
     gene            complement(6347..6739)
                     /locus_tag="PLQ77_06905"
     CDS             complement(6347..6739)
                     /locus_tag="PLQ77_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPL10441.1"
                     /translation="MKWYHVGIKAAKSEASVRFYRDILGLEVIDRVEIWGKQFNFVGN
                     DTISIEIEQANPGDTPSDPSSMTGLNHFSLVVDDIHALVEQARKKGAGVLMDPFYPRP
                     DRLTTFIRDPDGVMIQLIQFVSKDPAGG"
     gene            complement(6741..>7385)
                     /locus_tag="PLQ77_06910"
     CDS             complement(6741..>7385)
                     /locus_tag="PLQ77_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPL10442.1"
                     /translation="PHRHDSMRDQELPAKEFVDAVNALAKKHRFLILVDDVRCGFRLE
                     LAGSSMHYGFNADIQCFGKAMANGYAISVAAARKEMMDAAKTIFFTGTHYFSGVPFAA
                     ALACIDEIQASGAIEYIRKTGTQLMDGLRQAANAAGLQVSVTGPPAMPYMTFADDPLF
                     EKNRFFCGEAARRGIFFHPHHNWFVCAAMTESDILRTIDVASECFKLAKEKFGG"
BASE COUNT         1636 a         2152 c         1977 g         1620 t
ORIGIN      
        1 ttccgacgct gcgcggcaaa agcgtgatca acctgtttta tgaagccagc acgcgaaccc
       61 ggacgtcctt tgaaatcgcg ggcaaaagac taagcgccga caccatcaac attgccgcgt
      121 ccaccagctc cgcggtcaag ggcgaaaccc ttctggatac cgcgcgtacg ctggaagcca
      181 tgaatcccga cattatcgtg ctgcggcaca gcgttgccgg aacgccgcac atgctcgccc
      241 gcatggtgaa gcagtccatt atcaacgccg gcgacggcgc gcatgagcac ccctcacagg
      301 cgcttctgga catgatgacg atccgggaga agaaaggccg gattgccggg ctgaaagtcg
      361 cgatcgtcgg cgatattctg cacagccgcg tcgcacggtc caacatttac ggcctttcca
      421 ggatgggcgc ccgtgtggtc gtggccggac ccgccaccat gatgccgcgc ggcatcgaac
      481 ggatgggggt cgaagccttc acgaccatcg aagatgcgat cgccggcgcc gacgtcgtca
      541 tgatgctgcg cattcaactg gagcgccaga agcagaacat cttcccctcg cttcgcgaat
      601 actcccatca ttactgtctc agccgccggc acatgaagct caccgcaccc gacgccatcg
      661 tcatgcatcc gggtccgatc aaccgcggcg tggaaatctc tcccgatatc gccgacgacc
      721 ccgcctgctc cgtcattctc gaccaggtca accacggcgt ggccgtccgg atggccatgc
      781 tgtacctgct ttcaggagga aacgaatgaa actgctgttg aaaggcgccc gggtgattga
      841 cccggcacaa aacctggacg cactgatgga tgtgctggtg gaaggaggaa aaatcgccgc
      901 ctgcaaaccg ggcatcaaag ccccggcggg cgccaaagtt ctcgaactgt ccggaatgct
      961 ggtcacgccc ggactgatcg acatgcacac gcatctgcgc gaaccgggat atgaataccg
     1021 ggaaaccatc gccagcggct ccgccgccgc tgttgccgga ggattcactt caattgcctg
     1081 catgcccaac accctgccgg tgaatgacaa ccgctcggtc accgaattca tcctgcggaa
     1141 ggccgccgaa gcgaatctgg ccaatatcta tcccatcggc gcgatcagca tgggctccga
     1201 gggcaaacaa atgaccgaat tctgggacct cagggaggcg ggcatcgccg ctctgtcgga
     1261 tgacggcaac ccggtgatgg acgcggccct gatgcgcagg gccatggaat acgcctattc
     1321 cctggagctg ccggtgatcc agcactgcga agacaagaac ctctcggcgg gcggcctgat
     1381 gaatgaagga taccaggcca cggtcctggg gctgccgggc attcccgcca tcgccgaaga
     1441 agtcatggtg gcgcgcgaca tcctcatcgc cgagtacacc ggcacgcgca ttcacttcgc
     1501 gcacgtgagc acaaccggct ccgtccgcct gattcgcgcc gccaaaaaac ggaggctgaa
     1561 ggtgacggcg gaaaccgccc cgcattattt cactctgacg gatgaatcgc ttcggaatta
     1621 tgacacgaat tacaaagtca gtccgccgct gcggggtgac gccgatgtgt cggccatcaa
     1681 ggaaggcctc gcggacggaa cgctggacgc cattgcctgc gaccacgcgc cgcacggccg
     1741 tacggacaag gaagtggaat tcgaatacgc cgcctgcgga atcagcggcc tggaaacctc
     1801 tctgggcctg agtctgagcc tggtgcagga gggaattctg ccgtggccgg agctgattgc
     1861 caaaatgagc tgcaatccgg cgcgcatttt gaacctgccc aaagggacgc tggcaaaagg
     1921 cgccgacgcg gacattacgg tgatccatcc ggaccttgcc tggagcgtcg atgtcgccgc
     1981 ctttcattca ctgggaaaga actcaccgtt ttccggaaga aagctgaaag ggcgcgccgt
     2041 tttaaccatc gtgggcggcg acatcaagta cgatgggcgg gcatcccagc cgtgagcgcc
     2101 cgggaaaccc ataagaccgc cggctttgtc ctgcgaagcc ttcagtacgg ggaatcggat
     2161 ttgatcgtca cgttttacac ccgtgagttc ggcaagatca aaggtattgc caagggcgcc
     2221 aaacgcagcc gaaaacggtt cgccaacgtc tttgaaccct tctccctgac gcagatcatt
     2281 ttttcccgcc gaaaccgtga ctcgctcgcg tttattgacg cctgcgacat cctcgatcac
     2341 tttgccgcca tccgcatgga cctggaaaag accctgaccg cttcctattt tattgatctg
     2401 gccgaacact tcagcccgga gggcaagcgc aatgacgggg tctttgcatt gcttctggat
     2461 tttctgccgc ttctgacccg ggaaaacgta tcggagccga taattcgttt ctttgaaatg
     2521 cgccttttga aagccgccgg ttttgagccc gagcttgcgg cctgcgtacg ctgcaaaact
     2581 cccgtggcca acggttcccc ctactacttt ttcccgaagg aaggcggcat tttctgcacc
     2641 gcatgcgcaa gaccccaacg ctacgaccag agcatctccg caggcgccgt ccgcacgctt
     2701 cttctgggaa aagacatgga cctggaaaaa atgaaaatga tctcgatgac ggaaagtctt
     2761 gcatccgaat cccgcagtat tctctgcggc ttcatttcct acgtgctggg aagggaagtc
     2821 cagtctctga aagtcctgga gcaggtgcgc aggtactgtc cctgacggag gtttttttat
     2881 gcgccgaccg gaactgctgg cccccgccgg caacctggag aaactgcgca ccgccctgct
     2941 gtatggggcg gacgccgtct atgccggagt tcccggcctg agcctgcggg gcgcgcccgc
     3001 cgaaatgtcg ctggaggatc tggcctcggg tgtttccgaa gcgcacgctc aaggcatccg
     3061 ggtctatgcc gccgtcaaca cgtttgcccg caatgccgat ctgaagcaag cccggctgca
     3121 tcttccggat gtggccgcaa cgggggtgga cgcgctgatc gtcagcgacc cgggcctgat
     3181 ccggctggcc cgaaggactg ttccgcagat tccgcttcat ctcagcacac aagccaacac
     3241 caccaatgcc gacgcggtcc gcttctggcg tgacctgggc gtgagccgga tcattctcgc
     3301 acgggagctt ccccttgggg aagtggagga aatcgcctcg gctctccccg atgtggagct
     3361 ggagattttc atccacggcg caatgtgcat ggcctattcc ggacgctgtt atctgtcggc
     3421 ctggcgaaac cgcaggagcg ccaacgaggg agactgcacc caaccctgcc gctgggagta
     3481 ccggctttcg gaatccaccc gcccggaaga tcctattgtg gtggaagaag acgaacggtt
     3541 ctcgtatctt ttgagttcaa aagatttatg tctgctggag catctgcccg aggtcctggc
     3601 gaccggcgtc gccggcgtca agattgaggg aagaatgaaa tccgcctatt atgcagccgt
     3661 cgtgacccga acctaccggc aggcgcttga cgccctttgc cgtgacaaag aagcctaccg
     3721 ctgcgatccg cggtggatcg aagagctcaa aaccgtctct caccggggct acacgaccgg
     3781 attcgccttt gccgaagaaa agatcaatga atccagtccg gatgtcaaaa acattcagac
     3841 gcatgaaccg gcggggatgg tggtcggcta tgacggcatc aggaaacaaa tgcggatgga
     3901 agtccgcaat ttcctgcagg cgggcgaaga gatggaattg ctgcttatgg aggaaaccct
     3961 gcccgtctcc gcccagccga tgacggatga ccggggaagc ccaatcgacc gggcccacgc
     4021 gggaaacctc attcgcgttc cccttccctt cccggcgccg accggcgctc tgcttcggaa
     4081 aaaaaccgtc ggggcaaaaa cggaagagac aaagcgcaaa ccttctgaaa acggctgacg
     4141 atcagatcac ttctttttct tctttgctgc gggctttttt gcggcgggct tttcctcggc
     4201 gacgcaaatg cttttctgca gcgtatgatg atccgttaca aatttaattt tttcccaggg
     4261 ttcattttcg taggtgtgat gatccagaac ctttcccttt tcgtcgtaat ggatcagctc
     4321 tttcacccgc catttttgat tcctgcaatc aatctcatcc acgcggacat catgttcgta
     4381 gtaatgatac ttgatggatt tcttcgggtt gtatttcctg acttcttcaa ccatctgctt
     4441 cctgcaagaa tccgttacga ctctgtaaac gttaacggtt gccacatcgg aggatctggt
     4501 aatattggtt ttattgtaat agttttctcc catcttggtt tttccatagc gcacccatac
     4561 cttgctgtcc ggaatgcttc gcgcaatttc gggcagtttt tcgatggcgg gagctggagg
     4621 aggcggggca acggggggtt ttttcacaac gaccggggcc gggggagttt ctttgagttt
     4681 cggctcttct tttttggtaa cggacggacc tttcgcgcag cccgcaagca gaaaaacgat
     4741 cagaaaaaaa tataaaacca aatggatcct tttcatgtct gctcctttct tcattcttcc
     4801 atgccccggc caatccggtc atccctcgtc cacgagcggc aatgaaagcg gtcctgataa
     4861 aattgcttgt gaaatttaat acatttatga tgatttatca aggatctctc acatccagcc
     4921 tgaaaaaaat ggtcccccgc cgggtgcgcc aaacccggtt tcccaataaa gaaagccccc
     4981 ccggcaaaac cgggggggcc tcattggaac cgaaagcctt tcagacgaaa gacttcatca
     5041 tctgctattt cttataatcg acggccgctt ccacgcggcg attctttgcg cgccccgctt
     5101 tggtcttgtt ggaagcaacg ggtttttcct ggccgtatcc gacggcgttc agacgctggg
     5161 cgtcaatgcc gcctttttca accatgtaat tcttaacggc ttccgcgcgt ttctgggaaa
     5221 gattcttatt gtactcggcc ttgcccacat tgtccgtatg gccttcgatg gtgagattca
     5281 cgtcagaatg ttctttcatg acaccaacca tgtggtcgat atccttggtg taattctttt
     5341 tgatggtggc cttgtcgaaa tcaaacagga cctccagcgt agctcttccc atctcatcga
     5401 cttcttcgcg cacctgagtc ctgggctggg gtctcacgac ttcctcggta accacggggg
     5461 ctgccgccgc gacggcgggt ttcgcctctt caactaccgg tgcggcctcc gcctggggtt
     5521 ttgcttcttc cataaccggc tcggctgcgg aaacaggcgt tgcaactgct tttttggcgc
     5581 ctccaaaagc aaaattcaaa ccgagagata cgagcatgtt gttgtgaagc ttttcgatcg
     5641 gcagcacatg gcgaatgtca aggcgaagcg cgaaccaatc cgacaggaac cacttcaaac
     5701 cgtagccata atcaaaagcc agcttgtctt ttttgtaatt gtcggtcccg taatctgaat
     5761 catcctcgaa tacaccgtag gaatagtgaa cgccgccaac accggccgcc aggaaaggca
     5821 ccagcgcctg attcggcaga agattgaaaa taacctctgc gccgtatccc gtgaaatcca
     5881 cattctgctt gggagaccag gcgttgttat ctgtcttggc ataatggaca aaaccttcga
     5941 cgccgagata tttgttgaaa ttgtagccaa ggcgcatccc gaccagcggc attgccctat
     6001 ccatgtcttc gttgccttca aacacataca tgcccaaggt cggcgcaacc gtaaaagacc
     6061 cggacctcac ctgcgcgttg cctgtggaga ccagcaacat caccagcagg acaacaacca
     6121 atgaagcgaa ttttttcata atctttctcc ttaaaataaa cgtattttga attgcactcg
     6181 aactttgttt aatcaggcca acccgcccat attccaaaca aacaattaaa aactttcgaa
     6241 tttactgaca cattgtgacc aatgcttcta taatcaatca cttaatgtat gtcaatataa
     6301 tttttcattt atatttatca ccgaaaaacg cctgatcctt cccggtttac cctccggccg
     6361 ggtcttttga aacaaattga atcagctgga tcatcacacc gtcgggatcc cggataaagg
     6421 tcgtgaggcg gtccggccgg ggataaaacg gatccatcag gacgccggcg ccttttttgc
     6481 gggcttgctc cactaatgca tgaatatcgt ccacgaccag gctgaaatga ttgagaccgg
     6541 tcatggaaga aggatctgat ggggtgtcgc cgggattggc ctgctcaatc tcgatggaaa
     6601 tggtgtcatt tcccacgaaa ttgaattgtt ttccccagat ttccacccga tcgatcactt
     6661 ccagacccag aatgtcccgg taaaatcgga cggatgcctc acttttggcg gctttgattc
     6721 cgacatgata ccatttcata tcagcctcca aacttttctt ttgcaagttt gaaacactcg
     6781 gaggccacat caatggtcct gagaatatcc gattccgtca tggccgcgca gacaaaccag
     6841 ttgtggtgcg gatggaaaaa gatgccgcgg cgggccgcct caccgcagaa aaaccggttt
     6901 ttttcaaaaa ggggatcgtc ggcaaaggtc atgtagggca tggccggggg accggtgacg
     6961 ctgacctgca ggcccgccgc gttcgcggcc tggcgaaggc cgtccatgag ctgcgtcccc
     7021 gttttgcgaa tgtactcgat ggcgcctgaa gcctgaatct cgtcgatgca ggccagcgcc
     7081 gcggcaaagg gaacgcccga gaagtaatgt gtgccggtaa aaaaaatggt tttcgccgca
     7141 tccatcattt ctttgcgcgc ggccgccacg gaaatagcgt atccgttggc catcgccttg
     7201 ccgaagcact ggatatcggc gttgaatccg taatgcatgg agcttcccgc cagttcaagg
     7261 cggaaaccgc agcggacatc atcgacaagg atcagaaacc ggtgcttttt cgccagggcg
     7321 ttgacggcgt ccacgaattc ctttgccggc agctcctggt ccctcatgga atcgtgccgg
     7381 tgcgg
//
LOCUS       DAOPAF010000074         7293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9195_length_7293_cov_44.169246, whole genome shotgun sequence.
ACCESSION   DAOPAF010000074 DAOPAF010000000
VERSION     DAOPAF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_7293_cov_44.169246"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLQ77_06915"
     CDS             complement(<1..811)
                     /locus_tag="PLQ77_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL10443.1"
                     /translation="MNNSGLTPREIVERLDRHIIGQADAKRAVAIALRNRWRRQNVPE
                     DLADEISPKNIIMIGPTGVGKTEIARRLAKLDNSPFLKVEASKFTEVGYVGRDVESMV
                     RDLVELSINMLKAEEQDHVRARAAEIAEDRLLDLLLPPRPAERNVGDMLTDAEPEQAS
                     LPLNEEAARRQDATREKLRKLLHDGKLDERMVELEIAEPRSGPMIEIFSAAGMEDMGL
                     NLKDMLGSMMPQKKKKRNVKVPEALEIIASEEAQKLIDMDKVTKTALDRVEQ"
     gene            complement(830..1405)
                     /gene="hslV"
                     /locus_tag="PLQ77_06920"
     CDS             complement(830..1405)
                     /gene="hslV"
                     /locus_tag="PLQ77_06920"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPL10444.1"
                     /translation="MKIRGTTILAIRKGDKITVAGDGQVTLDTTILKHGARKVRRLYN
                     NEVIVGFAGATADAFTLFDRFDQKLEQYNGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VSRDYSLIISGNGDVIESDDDVMAIGSGGAFAQAAARALVRHSNLTATEIAREAMKIA
                     SEICIYTNDHVTIEEIDLSEEKPKEDKKSKK"
     gene            complement(1519..2427)
                     /gene="xerC"
                     /locus_tag="PLQ77_06925"
     CDS             complement(1519..2427)
                     /gene="xerC"
                     /locus_tag="PLQ77_06925"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPL10445.1"
                     /translation="MKELLAAFRTFLDVQRNVSEHTRKAYLTDIEEYGEFLRNSSAQK
                     SVADADTDTVRSYMAFLHGRKLKKSSINRKISSLRAFYRFLVREGTVRNNPAQDVQTP
                     KTEKYMPTFLSVDEAFDLLEASGSDPSLSGLRNLAMLELFYSSGLRLAELAGLDEDDV
                     NFSAALVKVRGKGKKERIVPVGKRALEALKRYLSATEDTRRKLFREGMGGALFLNERG
                     GRITTRSIARIVDTATVKSGIGRKISPHALRHSFATHLLAAGADLRSIQEMLGHESLS
                     TTQKYTAVNINRLMEVYDKAHPRAKK"
     gene            complement(2434..2934)
                     /locus_tag="PLQ77_06930"
     CDS             complement(2434..2934)
                     /locus_tag="PLQ77_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPL10446.1"
                     /translation="MLLEMKVSGLTIDPITNTPIVILKDLQESKAIPIWIGLFEASAI
                     ATELEHVVFSRPMTHDLMNEFLKALDVTVNRIEIVDIRNNTFFAAVYLVRDGQTYRVD
                     ARPSDAIALALRAGASIFVEESVLEKSRNIDFGIKLADIDKFKEDKVREFLENLSAED
                     FGKYKM"
     gene            complement(2946..4271)
                     /gene="miaB"
                     /locus_tag="PLQ77_06935"
     CDS             complement(2946..4271)
                     /gene="miaB"
                     /locus_tag="PLQ77_06935"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPL10447.1"
                     /translation="MKNKYLYIETFGCQMNVHDSGQMAVLLRDLGYEPTEDSAKADCI
                     LINTCSIREKAEQKAMSELGRLMKLKEKNPNLIVGFAGCLAQHLGARVHGRVKNVDLV
                     FGTHNIHRLPEMMARVQKERKPVTEVGFSSSLHSLGIFAPPPKGAVGAFVSIMQGCDN
                     YCAYCVVPFLRGPEMSREPKDIVGEIEKLAGRGIREVTLLGQNVNSYGKKTGGRGDFV
                     FLLKKINDIEGIERIRFTTSHPRDLSEELISCFGSLRKLCRHIHLPVQSGSSRILKRM
                     NRGYTAEEYLEKVARLRRVCPEISITSDIIVGFPGETQKDYQETIDMMEKIRFDSVFS
                     FKYSERKGTAAQAIEGKVPETEKKLRLREVQALQDRHTQEKNTSLEGSVQEVLVEGQS
                     RNSRQDMTGRTSSWKIVNFEGRSELIGRTVPVRISRGYLHSLRGKQITP"
     gene            complement(4328..5200)
                     /gene="rsmA"
                     /locus_tag="PLQ77_06940"
     CDS             complement(4328..5200)
                     /gene="rsmA"
                     /locus_tag="PLQ77_06940"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPL10448.1"
                     /translation="MTTPGEILRRYGIRPDKRRGQSFLTDSNTVRKIAQAAKAAPEDI
                     VVEIGAGFGVLTRQIAGQAGRLIAVEIDARLVEILQKELAEFERVEIFQNDILKFDFS
                     YISKQYKSRLKVVGNVPYNISSPVVFHLLAHRSAISEFTLMLQKEVVERLISPPGHKS
                     YGVPSVLLQMHADVERLFDVSATCFYPRPKVESSVIHGTFRDQPLVELTDEAFFRRIV
                     KAAFAQRRKMLFNNLKHAQILENMSGAEIQSALDESGIDGKRRGETLSLKEFALLSNI
                     LKSKAQPSGEFVNG"
     gene            complement(5208..6233)
                     /gene="tsaD"
                     /locus_tag="PLQ77_06945"
     CDS             complement(5208..6233)
                     /gene="tsaD"
                     /locus_tag="PLQ77_06945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPL10449.1"
                     /translation="MFVLGIESSCDETAAAVVRDGKLLSGVIASQIKDHSAYGGVVPE
                     LASRKHIEAIVPVVEQSLKEAGASFRDLEGIAVTRGPGLIGSLLVGLSAAKALAYALG
                     IAFVGVNHLEAHIMASSLGEKKPRFPFAALVVSGGHTNVYSVKNYHRFALLGQTRDDA
                     AGEALDKAAKLLDLGYPGGVVIDRMARDGNPEAYGFPRAMKDSPDFSFSGLKTSLLVM
                     MKKRGKGFAESELPDVVASYQEAVVDVLVEKTLRAARENGLSQIVICGGVAANSRLRE
                     KFSEAATREGMELFFPPMILCTDNAAMVAALGEVMLENGRSDSLDLNAVSRWPLVPRF
                     PEENGKE"
     gene            6345..>7293
                     /locus_tag="PLQ77_06950"
     CDS             6345..>7293
                     /locus_tag="PLQ77_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPL10450.1"
                     /translation="METQTDFLIIGSGIAGLSLAIKVAQLGSVAIVTKKEKSESNTNY
                     AQGGIAAVTDKTDSFEEHISDTLECGAGLCRKDVVEFVVREAPPRVQELIEWGVNFTK
                     SEEPPHLYDLGQEGGHHRRRVLHAKDLTGQEIERALHEKVAALKNVRIYENHIGIDLI
                     LRKDPQGRTVNCLGAYVLDINEDEVRTFRAKYTILSTGGAGKVYLITTNPDIATGDGI
                     AMAYRAGAKIANMEFIQFHPTCLFHPEAKAFLISEAVRGEGGILKLKNGATFMEKYHP
                     MNSLAPRDVVAKAIDNEIKQSGDEYVLLDISHHSREFLLN"
BASE COUNT         1511 a         2218 c         1838 g         1726 t
ORIGIN      
        1 actgctccac gcggtccagc gcggttttgg tgaccttgtc catgtcaatg agcttctgcg
       61 cctcttcgga cgcgatgatc tcgagggctt ccggcacctt gacgtttctt tttttcttct
      121 tctgcggcat catgctgccc agcatgtcct tcagattgag tcccatgtct tccatgccgg
      181 cggcggaaaa aatctcgatc atggggccgc tcctcggctc ggcgatttcc agctccacca
      241 tgcgttcgtc gagtttcccg tcgtgcagaa gttttcgcag cttttcgcgc gtcgcgtcct
      301 gcctgcgcgc cgcctcttcg ttgagcggca gactcgcctg ctccggttcc gcgtccgtca
      361 gcatgtcgcc gacgttcctt tccgcgggcc gcggcggcag cagcagatcc agcagccggt
      421 cttccgcgat ttccgcggct ctcgcccgca cgtgatcctg ctcctcggcc ttcagcatgt
      481 tgatcgaaag ctccaccaga tcccgcacca tggattccac gtcccgcccg acgtagccga
      541 cttccgtaaa tttcgaagcc tccaccttca gaaacgggga attgtccagt ttggcaagcc
      601 ttctggcgat ttccgttttg ccgactcccg tggggccgat catgataata tttttcggcg
      661 atatttcatc cgccagatct tccggaacat tctgcctgcg ccagcggttt cgcagcgcga
      721 tggccacggc ccgtttggcg tcggcctggc cgatgatatg ccggtccagc cgttccacaa
      781 tctcgcgggg tgtcaaacct gaattgttca tttttcttct gtgactcctt tactttttgc
      841 tctttttgtc ctctttgggc ttctcctcac tgaggtcgat ttcttcaatc gttacgtggt
      901 cgttggtgta gatgcatatt tccgaggcaa tcttcatcgc ttctctcgcg atttccgtgg
      961 cggttagatt gctgtggcgc accagcgcgc gggccgcggc ctgcgcaaaa gcgccgcccg
     1021 atccgatggc catcacgtcg tcatccgatt caatcacatc gccgtttccg gaaatgatga
     1081 gcgaatagtc ccggctgacc gcgatcatca gcgcttccag atgccgcagc acccggtcgg
     1141 tccgccagtc cttggtcagc tccacggcgg cgcgaagcag gttcccgttg tactgttcca
     1201 gtttctgatc aaagcggtca aacagcgtga aggcgtcggc ggttgcgccg gcgaatccga
     1261 caatcacctc gttgttgtac agccttcgca ccttgcgcgc gccatgcttc agaatcgtcg
     1321 tgtccagcgt aacctgaccg tcgccggcca ccgtgatttt atcgccctta cggatcgcca
     1381 gaatggttgt gcctctgatc ttcatttggt ctcccatact tcgcagctca taactgatgg
     1441 ctcatagctt atggtttgtt tctcttcact cattgctcat cactcgtctc tcgtcgctca
     1501 ttgctcgtca ctcatcgctt atttctttgc tctggggtga gccttgtcgt acacttccat
     1561 caggcggttg atgttgaccg ccgtgtattt ctgcgtcgtg gaaagactct catgccccag
     1621 catttcctgg atcgaacgca aatcggcgcc ggccgccagc agatgcgtgg caaagctgtg
     1681 ccgaagcgcg tgagggctga tcttccgtcc gatgccgctt ttgacggtgg ccgtatccac
     1741 gatgcgggca atgcttcggg tcgtaatccg ccctccccgt tcgttcaaaa aaagcgcgcc
     1801 gcccatccct tcacggaaaa gcttcctcct cgtgtcctcc gtcgcggaca aatatcgctt
     1861 cagcgcctcc agcgctcttt tgcccacggg gacaattctc tcttttttcc ccttgccgcg
     1921 caccttgacc agcgcagcgc tgaaatttac atcgtcctcg tccagaccgg ccagctccgc
     1981 cagcctgagc cccgaagaat agaacagctc cagcatcgcc agattgcgca gaccggacaa
     2041 agacgggtcc gagccggatg cttccaggag atcaaaggct tcatcaaccg acaaaaaagt
     2101 cggcatgtac ttctccgtct tcggcgtctg aacgtcctgg gccggattgt tccggacggt
     2161 tccctcccgc accaaaaacc tgtaaaaagc ccgcagggag gatattttgc ggttgatgga
     2221 cgactttttc agcttccggc cgtgcaaaaa cgccatgtac gaccggacgg tgtccgtatc
     2281 ggcatccgcc acgctttttt gagcggatga attgcgcagg aactcgccat attcctcgat
     2341 atccgtcagg taggccttgc gggtatgctc cgacacatta cgctggacgt ccagaaatgt
     2401 ccgaaaagcg gcaagcagtt ctttcatttc ttttcacatt ttatatttcc cgaaatcttc
     2461 cgcggataag ttttccaaaa actcccttac cttgtcttcc ttgaatttgt cgatgtcggc
     2521 aagcttgatt ccgaaatcga tgttgcggga tttttccagg acgctctcct ccacgaaaat
     2581 cgaggcgccg gccctcagcg ccagcgcgat ggcgtcgctg ggacgcgcgt caacccggta
     2641 ggtctgcccg tcccgaacca gatacacggc cgcgaaaaaa gtgttgttgc ggatatcgac
     2701 aatttcaatc cggttgaccg tgacgtccag ggccttgagg aattcgttca tcaggtcatg
     2761 ggtcatcggt ctggaaaaca ccacgtgctc cagttccgtg gcgatggcgc tggcttcgaa
     2821 caggccgatc cagatcggaa tcgctttgct ttcctgcagg tctttcagga tgacgatcgg
     2881 cgtgttggtg atcggatcga tggtcaaccc ggacaccttc atctccagca gcattcgcaa
     2941 cctccttatg gcgtgatctg ttttcccctc agtgagtgca gatatccccg cgatattctc
     3001 accgggaccg tccggccgat cagctccgaa cgtccttcaa aattgacaat tttccaggag
     3061 cttgttcgcc ccgtcatatc ctgcctggag ttccggcttt gcccttccac cagcacttcc
     3121 tggacgctgc cttccagcga cgtatttttt tcctgcgtgt gcctgtcctg aagcgcctgg
     3181 acttccctga gccgcaattt tttttccgtt tccggcactt tgccctcaat cgcctgtgcg
     3241 gccgttccct tgcgttcaga atacttgaac gaaaaaactg aatcaaacct gattttttcc
     3301 atcatgtcaa tggtttcttg ataatctttt tgggtttctc cgggaaagcc gacaatgatg
     3361 tcggacgtga tgctgatttc gggacacacc cgtcgcaggc gcgccacttt ttccagatat
     3421 tcttcggcgg tgtacccccg gttcatccgc tttaaaatcc ggctggagcc ggactgcacc
     3481 ggcaaatgaa tgtgacggca aagcttgcgc aggcttccga agcaggagat cagttcttcc
     3541 gaaaggtctc tgggatgcga cgtggtgaag cgaattcttt caatcccttc aatatcattg
     3601 atctttttca ataaaaacac aaaatcgcct cggccgccgg ttttttttcc gtaggaattg
     3661 acattctgcc ccaaaagcgt cacttcccga atgccgcgcc cggccagttt ttcaatttcc
     3721 ccgacaatgt cctttggttc ccggctcatt tccggtcccc gcaaaaacgg aacgacgcaa
     3781 taggcgcagt aattatcgca cccctgcata atggacacga aggccccgac cgcgcctttt
     3841 gggggcggcg caaagatgcc cagcgaatga agcgacgatg aaaagccgac ttccgtgacc
     3901 ggcttgcgct ccttttggac gcgcgccatc atttccggca gcctgtgaat attgtgcgtg
     3961 ccgaaaacca gatcgacatt cttcacgcgg ccgtgaaccc gcgcgcccag atgctgggcc
     4021 agacagccgg cgaaaccgac gatcagattc ggattctttt ccttgagctt catcaggcgg
     4081 cccagttcgc tcatcgcctt ctgttcggcc ttctcgcgaa tcgaacaggt gttgatgagg
     4141 atgcagtccg cctttgcgga atcttcagtc ggctcataac cgagatcacg gagcaaaacg
     4201 gccatctgcc cggagtcgtg aacgttcatc tgacaaccga aggtctcaat atataaatac
     4261 ttgtttttca tgaccatcct gtcatttatt tgacgtgagc attatatcac aggagaaaat
     4321 atccgtccta tccattaacg aattcgccgg atggctgggc ctttgatttc aaaatattgc
     4381 tcagcagggc aaactctttt aaagacaacg tttctcctct tcttttccca tcgatgccgg
     4441 attcatcaag agcggattga atttccgccc cggacatgtt ctccagaatt tgcgcatgtt
     4501 ttaaattgtt gaaaagcatc tttcggcgct gggcaaaagc cgccttcact attcttctga
     4561 aaaaagcttc gtccgtgagt tcaacgagcg gttgatcccg aaatgttccg tgaatgacgg
     4621 aagactcaac tttcggcctc ggatagaagc aggttgccga tacatcaaaa agcctttcca
     4681 cgtccgcatg catctgaagc aaaacggaag gcacgccgta tgatttatgc cccggcgggg
     4741 aaataagtct ttcaaccact tccttttgca gcatcagcgt aaattcggaa atcgccgacc
     4801 ggtgcgccag cagatgaaaa acaacggggc tggaaatatt gtacggaaca ttaccgacta
     4861 ctttaagcct tgatttatac tgctttgata tataagagaa atcgaatttt aaaatatcat
     4921 tttggaaaat ctccactcgc tcaaattccg caagctcctt ttggagaatt tccaccaggc
     4981 gggcatcgat ttccacggcg atgagcctgc cggcctgtcc ggcgatctgc cttgtcagaa
     5041 cgccgaagcc ggcgccgatt tccacgacaa tgtcttccgg cgctgccttt gcggcctggg
     5101 cgattttacg caccgtattc gaatccgtca ggaaggactg gccgcgtctt ttatcggggc
     5161 gaatcccgta ccggcgaaga atttccccgg gtgtggtcat agcatgctca ttccttcccg
     5221 ttttcctccg gaaaccgggg aaccagaggc catcgcgata cggcattgag gtccagtgaa
     5281 tcgcttcttc cgttttccag catgacctcc cccagggccg cgaccatcgc cgcgttgtcc
     5341 gtgcacaaaa tcatcggcgg gaaaaacagc tccatgcctt ccctggtggc cgcttcggaa
     5401 aacttctctc gtaaacggct gttggcggca acgccgccgc agatgacaat ttgagaaagg
     5461 ccattctccc gggctgccct gagcgttttc tccaccagga cgtccacaac cgcttcctgg
     5521 tagcttgcga cgacatccgg aagctcactc tccgcaaagc ccttcccccg cttcttcatc
     5581 atgaccaaaa gggatgtttt gagtccgctg aagctgaaat ccggcgaatc tttcatggct
     5641 ctgggaaaac cgtaggcctc cggatttccg tctctggcca tccggtcgat cacgacgccg
     5701 ccggggtagc ccaggtcgag cagtttggcc gccttgtcca gcgcctctcc cgccgcatca
     5761 tcccgcgtct ggcccagcag cgcgaaccgg tggtaatttt tcaccgaata cacattggtg
     5821 tgaccgccgg aaaccaccag agcggcaaac ggaaaccgcg gttttttctc ccccagcgaa
     5881 gacgccatga tgtgcgcctc caaatggttg acgccgacaa acgcaatgcc cagcgcatag
     5941 gccagagcct tggcggcgga gagcccgacc aggagcgagc cgatcaggcc cgggccgcgg
     6001 gtgacggcaa tcccctcgag atcccggaag gaagcgcccg cttccttcaa agactgctcc
     6061 accacgggaa cgatcgcttc gatatgcttt cgcgaggcga gctccggcac cacgcccccg
     6121 tatgcgctgt gatccttgat ctgcgacgca atcacgccgg agagcaactt tccgtcccgg
     6181 accaccgcgg ccgccgtttc gtcgcaggat gactcaatgc ctaaaacaaa caaggaaact
     6241 cctttttcat aattttcttt acaaaccgcg aaaaaggctt atataaaagc ccccgacatt
     6301 tgtaaacaaa cttttagcgg cgggaatgag cgggcttttc tcacatggaa acacaaactg
     6361 attttctcat catcggaagc ggaatcgcgg gtttgagtct ggccatcaaa gtggcgcagc
     6421 tcggttcggt cgccatcgtc accaaaaagg aaaaatcgga atccaacacc aactacgccc
     6481 agggcggcat cgcggcggtc acggacaaga ccgacagttt tgaagagcac atcagcgaca
     6541 cgctggagtg cggcgcgggc ctctgccgga aggacgtcgt tgaatttgtc gtgcgggagg
     6601 cgccgccgcg cgttcaggaa ctgatcgaat ggggcgtcaa tttcacgaaa tcggaagagc
     6661 ctccccacct ttacgatctg ggccaggagg ggggccatca ccggcgccgc gtgctgcacg
     6721 ccaaagacct caccggccag gaaatcgagc gggcgcttca cgaaaaagtc gcggcgctga
     6781 aaaacgtccg gatttacgaa aaccacatcg gcatcgacct gatcctccgg aaagatccgc
     6841 agggccggac cgtcaactgt ctgggcgcct acgtgctcga catcaacgag gacgaagtcc
     6901 gcaccttccg cgccaaatat acgattctat cgacgggagg cgcgggcaag gtctatctga
     6961 tcaccaccaa tccggacatc gccacgggcg acggcattgc catggcctac cgcgccggcg
     7021 caaaaatcgc caacatggag ttcatccaat tccaccccac ctgcctgttc catccggaag
     7081 ccaaggcgtt tttaatcagc gaggccgtcc gcggcgaagg cggcattctg aagctgaaga
     7141 acggcgccac cttcatggaa aaataccacc ccatgaacag ccttgcgccg cgcgacgtgg
     7201 tggccaaagc catcgacaat gaaatcaagc aatccgggga tgaatacgtc cttctggaca
     7261 tctcgcacca cagccgcgaa tttctcctga acc
//
LOCUS       DAOPAF010000075         7147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9412_length_7147_cov_61.420897, whole genome shotgun sequence.
ACCESSION   DAOPAF010000075 DAOPAF010000000
VERSION     DAOPAF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_7147_cov_61.420897"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLQ77_06955"
     CDS             <1..464
                     /locus_tag="PLQ77_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL10451.1"
                     /translation="VTEILNGSVRLKNQRVAVIGSGMTGLETAEKLAEEGNRVLVAEM
                     MDRIAPGVHPQHLDDILPRLKAYSADFITGHKLAKISAGVVFLESVAGGKRREEKADA
                     VVLSVGVQSNSALAAALQPHFPRLRVIGDARRVGRIPQAVRDGFDTAWNLM"
     gene            498..1028
                     /locus_tag="PLQ77_06960"
     CDS             498..1028
                     /locus_tag="PLQ77_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10452.1"
                     /translation="MSIIKDVQSGKKPLKRAYVAIFLWFTGRAIQAAAKVDSEVKKEF
                     EKLPEDFTFSLGCLPCGPWMVVGREKGVVKYLGGDSRGRPVQVRLAIKSLDAGFLILS
                     FQESTALATARNRLITDGEIPQALAIIRILDIVEVYLLPKLIASLAVKRYPSWWSFGR
                     KLGGRIGVYTRTLLGI"
     gene            1044..2210
                     /locus_tag="PLQ77_06965"
     CDS             1044..2210
                     /locus_tag="PLQ77_06965"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL10453.1"
                     /translation="MNSAAVFDFTCPVKTNSGNKALEHLPVELAGLNAVNPLIVANAR
                     AVGPKAVRTLMNAFGDSKMTLGLFEGVTEAADLGVVDHLKNICLEKKYDAFIALGGGK
                     AADVAKVLNLAVSLKTPDARQLSPKTPVRKRLLPLVVVPTAGADGLETSGFAHLNRMI
                     FSSEFLAPGLAVLDSRLARARKGILMAATGLAALGRAVESHVSADRNPFQKAYSFAAV
                     RFVRENLPAAVADPGNKKAALAVLNAAAMSGCAFSNAASGRLHKLGQVFYELLGIPQG
                     VIMGMCLSRVLEDGFREGSCDPAALLQPLAGDDAFARTPAGKRAEAALALLNRFLEEL
                     RGALGKDLPKSLSEAGVSGYRKEEILDVLGKDEDGHYLRGVVEGMAGGKRERKG"
     gene            2216..3463
                     /locus_tag="PLQ77_06970"
     CDS             2216..3463
                     /locus_tag="PLQ77_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL10454.1"
                     /translation="MLLPDYYEFCCRVKIVAGHDALEKIPDLLIERGAKKPLVITDKG
                     VAGAGLIDIVSRALGDRVQADLIADDVPPDSDLRVVNRLARLYRDNACDAIIAVGGGS
                     VMDTAKGINIVVSENAEDLMAFSGVGALKRPLKPLVAVPTTAGTGSEVTQVAVIKDHE
                     KHLKMAFGSYFLLPDISILDSRMTLTMPPFITAMTGMDAMSHAVEAYYCLQKNPLSDA
                     HALSAVGMIYRHLPLVVKNPDDREGRLAMAVAACLAGIAFSNSMVGLVHNLGHATGSV
                     CGVPHGLCMAILLPYGLEYNLHKMADVMAELLLPMAGEQVYLATPAEQRAQKVVSLIR
                     GLNEDLHAITGGRHARCLQEIKDREGKPSVPRDVLPQIAAAALLDGASIYNPEDTDYE
                     DNLMVLEAAWEGKPLDRSRVKKG"
     gene            3483..5429
                     /locus_tag="PLQ77_06975"
     CDS             3483..5429
                     /locus_tag="PLQ77_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPL10455.1"
                     /translation="MIKPYMGKILMVDLSSRTMTEEEIPDRVYEQYLSGMGLGAYILY
                     NRMPAGADPLGPDNILGFCSGFLTGTGSLFSGRWTLVGKSPLTGCWGDANCGGTFSPA
                     IKHCGYDAIFFKGISSGPVYLYVKNGKASLKDAADLWGRDAVETEEALKKQYGPKAAV
                     AVIGPAGEKVSLISGVCSDKGRIAARSGLGAVMGVKKLKAVVLDGVQRIGAHDPAEMK
                     RLSRHCLKWVNFPLPLPGWMLGFVGAFLRVTPIVFPQDGILYTSILRKWGTGGMNQTS
                     IEMGDSPLKNWDGSNRDFPMSRSRTINPDIVRRMEIVKYHCYSCPVGCGGICAAKGKY
                     PHTHKPEYESVLALGGLCMNEDLDSIFYLNELLNRAGMDTISAGATAAFAVECYEKGI
                     LTKEDTGGLELKWGNTEAMVALIRKMVDREGIGDLLADGTKIAAQKIGKGSEAFTVHA
                     GGQEPAMHDGRCDPGFNVHYSLEPTPGRHTLGAHLYYEMFQLWKRVKGIPKPLPLFIK
                     NSKFRVDGEKARMSAACSKFMSLANGAGMCMFGLFVGIKRVPTFDWLNAATGWNRTPE
                     EYMTVGERIQTLKQAFNVKHGVEPKNNFISSRALGTIPQAEGANKGRSVDIEKLASGY
                     WEQFGWDAATGKPKEETLRKLEIQ"
     gene            5440..5892
                     /locus_tag="PLQ77_06980"
     CDS             5440..5892
                     /locus_tag="PLQ77_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL10456.1"
                     /translation="MAYTITEKCNGCGACARLCPAGAITGEKKKLHAIEAVLCIECGA
                     CGRICPQAAVLDAAGAPCAMIKRSEWPKPEILLKACIACTVCVDACPVGCLAMSDLSR
                     DKGVDAFPHLRDSKACIGCGFCSRECPADALVMKKPVPAAQTVAAESA"
     gene            5889..6980
                     /locus_tag="PLQ77_06985"
     CDS             5889..6980
                     /locus_tag="PLQ77_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HPL10457.1"
                     /translation="MSIETIPRYPIDKIISGGQTGADRAALDFAIARNLPYGGWLPRG
                     RKTEDGALDLKYRLQEMPTTDYAKRTEQNVIDADGTVIVSHGFPVAGSALTLEFTRRH
                     DRPCLHIDLKEISPEKAAEVLVSWLGENRIRVLNVAGPRAGRDPAIYPAVMDLLERSI
                     ENAGPVDVVLFDFGGVLAEEGWKDGLQAIAHANGLKAVELLLIAADTVYETGYILGKG
                     SEISYWDAMRRKTGIRGDYAQLRDEILSRFILRENMIDLVKRLKADNLVVGILSDQTD
                     MLDQLNARMNFFQWFDYVFNSYHMGKGKRDATLFDDIAQRLKTLPGRILFIDDDPGNV
                     ERAKQRGWKAILYSDENVFQSELDRLLSA"
BASE COUNT         1513 a         2047 c         2202 g         1385 t
ORIGIN      
        1 ccgtcaccga aatcctgaac ggctccgtcc ggcttaaaaa tcagcgggtc gccgtgatcg
       61 gctcggggat gaccggcctg gaaacggcgg agaagctggc ggaggaaggc aaccgcgtcc
      121 tggtggcgga aatgatggac cggatcgcgc cgggcgtcca tccgcagcat ctggacgata
      181 tccttccgcg cctgaaagcc tattccgcgg atttcatcac cggccacaag cttgccaaaa
      241 tttcggccgg cgtcgttttt ctggaaagcg ttgccggcgg aaaacgccgc gaagaaaagg
      301 ccgatgccgt tgttctttcc gtcggcgtgc aaagcaacag cgcgctggcc gcggcgcttc
      361 aacctcactt tcccagactc cgcgtcatcg gcgatgctcg cagggtcggc cgcattcccc
      421 aagccgtgcg ggatggtttt gacacagcct ggaatttgat gtaaaagaaa aaacccatta
      481 gacaggagga tcggatcatg agcatcatca aggacgttca atccgggaaa aagccgctca
      541 agcgggcgta tgtagccatt tttctgtggt tcaccggccg cgccattcag gcggcggcaa
      601 aggtcgattc cgaggtcaaa aaagaattcg aaaaacttcc cgaggatttc accttttctc
      661 tgggctgtct gccctgcggc ccctggatgg tggtgggccg ggaaaagggc gtcgtgaagt
      721 atctgggcgg agattcccgc ggacggcccg tgcaggttcg cctggccatc aagagcctgg
      781 acgcgggctt tctgattctc tcctttcagg aaagcacggc gctcgccacg gcgcgcaacc
      841 ggctgatcac ggacggggaa attccccagg cgctggccat tatccgcatt ctcgatattg
      901 tggaagttta ccttctgccc aagctcatcg cctctctggc cgtgaagcgg tatccttcct
      961 ggtggtcgtt cggccgcaaa ctcggcggcc gcatcggtgt ttacacccgc acgctgctgg
     1021 gcatctaacg aaaggaatac gatatgaatt ccgcagccgt ttttgatttt acctgccccg
     1081 tcaagaccaa ttccggaaac aaggcgctgg agcacctgcc cgtggagctg gccggactca
     1141 atgccgtgaa ccctctgatc gtcgccaacg cgcgggcggt cggccccaaa gccgtccgga
     1201 cgctgatgaa cgcattcggc gattcgaaaa tgacgctggg cctttttgaa ggcgtcaccg
     1261 aagcagccga tctcggcgtc gtggatcatt tgaaaaacat ctgcctggaa aaaaaatacg
     1321 atgccttcat cgcgctgggc ggggggaaag ccgccgatgt cgccaaggtg ctgaatctgg
     1381 cggtttcgct gaaaacgccg gacgcgcggc agctttcgcc caagacgccc gtccgcaagc
     1441 gcctgctgcc gctggtggtc gtgccgacgg ccggagcgga cggcctggaa acctcgggct
     1501 ttgcccatct gaaccggatg attttttcct ccgaatttct cgcgcccggc ctggccgtgc
     1561 tggattcgcg cctggcgaga gcaagaaaag gaatcctgat ggccgcgacg ggccttgccg
     1621 cgctgggccg cgccgtggaa tcgcacgtca gcgccgacag aaaccctttc cagaaagcct
     1681 attcctttgc cgccgtccgc tttgtccggg aaaaccttcc ggccgcggtc gccgatcccg
     1741 ggaataaaaa ggcggcgctt gccgtgctca acgccgcggc gatgtccggc tgcgcctttt
     1801 ccaatgcggc ctccggcagg ctccacaaac tcggtcaggt gttttacgaa ctgctgggca
     1861 ttccccaggg cgtgatcatg ggcatgtgcc tgtcccgcgt tctggaagat ggctttcggg
     1921 aaggctcctg tgacccggcg gctcttcttc aacccctggc cggagacgac gcgtttgcgc
     1981 gaacgcccgc cggaaagagg gcggaggccg ctcttgccct cctgaaccgt tttctggaag
     2041 agcttcgggg cgctctgggc aaggatctgc cgaagagcct ttcggaggcg ggagtgtccg
     2101 gctaccggaa ggaggaaatt ctggatgtgc tgggaaaaga cgaagacggc cattacctgc
     2161 gcggcgtcgt ggaagggatg gccggaggaa aacgggaaag gaaggggtga ttccgatgct
     2221 tttgccggat tattatgagt tctgctgtcg tgtgaaaatt gtcgcgggtc atgacgccct
     2281 ggaaaaaata ccggatctgc tcatcgagcg cggcgcaaag aaaccactcg tcatcacgga
     2341 caagggagtg gcgggcgcgg gtctcatcga tatcgtttcc cgggcgctcg gggaccgcgt
     2401 gcaggcggat ctgatcgccg acgatgttcc ccccgattcg gatctccggg tcgtcaaccg
     2461 gctggcgcgc ctttaccggg acaacgcctg cgacgcgatc atcgccgtcg gcggcggttc
     2521 cgtcatggac acggccaagg ggatcaacat cgttgtttcg gaaaacgcgg aagacctgat
     2581 ggccttcagc ggcgtcggcg cgctcaagcg ccccctgaag cccctggtgg ccgtcccgac
     2641 caccgccggc acggggtcgg aagtcacgca ggtggccgtt atcaaggatc acgagaagca
     2701 tttgaagatg gcctttggga gttattttct gctgcccgac atttccatcc tcgattcccg
     2761 aatgaccctg acgatgccgc ctttcatcac ggccatgacc ggcatggacg ccatgagcca
     2821 cgccgtggaa gcctactatt gccttcagaa aaatccgctc tccgacgccc acgcgctttc
     2881 cgccgtcggc atgatttacc ggcacctgcc gctggtcgtg aaaaatccgg atgatcgcga
     2941 aggccgcctg gccatggcgg tcgccgcctg cctggcgggc atcgcctttt ccaattccat
     3001 ggtcggactg gttcacaatc tcggccatgc gaccggctcc gtctgcggcg ttccccatgg
     3061 cctctgcatg gccatccttc tgccctacgg cctggagtac aacctgcaca aaatggcgga
     3121 cgtcatggcg gagctcctgc ttcccatggc gggagagcag gtgtatctgg ccacgcctgc
     3181 ggagcagaga gcccaaaaag tcgtatccct gatccgcggg ctcaatgaag acctccacgc
     3241 catcaccggc ggacggcacg ccagatgcct gcaggagatc aaagaccgcg aagggaagcc
     3301 gtccgtcccc cgggacgtgc tgccgcaaat cgccgccgct gcgctgctcg acggagcgtc
     3361 tatttacaat ccggaagaca cggactacga ggacaacctg atggtgctgg aagcggcctg
     3421 ggaagggaag cctctggaca ggtctcgcgt aaaaaaaggc tgatgaacga aagggagcag
     3481 tcatgataaa accttacatg ggaaaaatcc tcatggtgga tttgtccagc cggaccatga
     3541 cggaagaaga aatccccgac cgggtgtatg aacagtatct gtccgggatg gggctgggcg
     3601 cttatatcct gtacaaccgg atgcccgccg gcgcggatcc gctgggcccg gacaacatcc
     3661 tcgggttctg ttcgggattt ctcacgggaa ccggcagtct gttttccggc cgctggacgc
     3721 tggtgggaaa atcgccgttg accggttgct ggggcgacgc aaactgcggc ggcacctttt
     3781 ctccggccat caagcattgc ggctatgacg ccattttctt caaaggcatc agctccggcc
     3841 ccgtctatct gtatgtaaaa aacggcaaag cgtctttgaa agacgcagcg gacctctggg
     3901 gacgcgatgc cgtcgagacg gaagaagcgc tcaaaaagca gtacggcccc aaagcggccg
     3961 tggccgtcat cggcccggcg ggcgagaagg tttccctgat ttccggcgtt tgcagcgaca
     4021 agggccggat cgccgcccga tcgggtctgg gtgccgtcat gggcgtcaag aaactcaaag
     4081 cggttgttct cgacggcgtt cagagaatcg gcgcgcacga tccggcggaa atgaaacggc
     4141 tgagccggca ttgcctgaag tgggtcaatt tccccctgcc gctgccgggg tggatgcttg
     4201 gttttgtggg tgcttttctg cgcgttacac ctattgtgtt cccccaggac ggcatcctgt
     4261 acacctccat cctgaggaag tggggtacag ggggaatgaa tcagacgtcc attgaaatgg
     4321 gagattcgcc cctgaaaaac tgggatggct ccaatcgcga ttttcccatg tcgaggtccc
     4381 ggaccatcaa tccggatatc gtcaggcgta tggaaattgt gaagtatcat tgctactcct
     4441 gccccgtggg atgcggcggc atctgcgccg ccaaaggaaa atatccccac acccacaaac
     4501 ccgaatatga aagtgttctg gccttgggcg gattgtgcat gaacgaggac ctggacagta
     4561 tcttttactt gaatgagctt ttgaaccgcg ccggcatgga tacaatttcc gcgggcgcaa
     4621 cggccgcatt tgccgtagag tgctacgaaa agggcattct caccaaagaa gacacgggcg
     4681 gtctggaact gaaatggggg aacacggagg cgatggtcgc cctcatccgg aaaatggttg
     4741 accgggaggg aatcggcgat cttctggccg acggcacgaa aatcgccgcc caaaaaatcg
     4801 gcaaaggatc ggaagccttc accgtccacg cgggcggaca ggaaccggcc atgcatgacg
     4861 gccgctgcga tccggggttc aacgtccatt acagcctgga gccgacgccg ggccgtcaca
     4921 cgctgggcgc ccatctgtat tatgaaatgt ttcagctctg gaagcgcgtg aaagggattc
     4981 ccaaaccttt gccgcttttc atcaagaaca gcaagttcag ggtcgacgga gaaaaggcgc
     5041 ggatgtccgc cgcgtgcagc aagttcatga gtctggccaa cggcgccggc atgtgcatgt
     5101 tcggtctctt cgtcgggatc aaaagagttc caacctttga ctggctcaac gcggcgacgg
     5161 gctggaaccg gacgcctgaa gagtatatga ccgtcggcga acggattcag accctgaagc
     5221 aggccttcaa tgtcaagcac ggcgttgagc cgaaaaacaa tttcatcagc agccgcgctc
     5281 tgggcacgat tccccaggca gagggcgcca acaagggaag atccgtggac atcgagaagc
     5341 tggcgagcgg atactgggag cagttcggat gggacgcggc caccggcaag cccaaagagg
     5401 aaaccctgcg caaactggaa atacaataag aggttgaata tggcctacac gatcacggaa
     5461 aaatgcaacg gttgcggcgc ctgcgcgcgc ctgtgcccgg ccggcgccat cacgggcgaa
     5521 aaaaagaagc tccatgcgat cgaagcggtt ttatgcattg aatgcggcgc gtgcggccgg
     5581 atctgtccgc aggccgccgt gctggacgcg gcaggcgctc cgtgcgcgat gatcaaacgc
     5641 tccgaatggc ccaagccgga gattctgctg aaggcctgca ttgcctgcac cgtctgcgtt
     5701 gacgcctgtc cggtgggatg tctggcgatg tcggacctct cccgcgataa aggcgtggac
     5761 gcgtttccgc atctgaggga ctccaaggcc tgcatcggct gcgggttctg cagccgggaa
     5821 tgccccgcgg acgcactggt gatgaaaaaa ccggtccccg ccgcccaaac ggttgccgct
     5881 gaatccgcat gagtattgaa accataccgc ggtatccaat cgacaaaatc atttccggag
     5941 gccagaccgg cgcggaccgg gcggccctgg actttgccat cgcccgcaac cttccttacg
     6001 gcggctggct tccccggggg agaaagacgg aggacggcgc cctggatttg aagtatcgcc
     6061 tgcaagagat gccgacgacg gactatgcca aaaggacgga gcagaatgtc atcgacgccg
     6121 acggcaccgt catcgtctcc cacggatttc cggttgcggg atcggcgctg acgctggaat
     6181 tcacccggcg gcacgacaga ccatgcctcc atatcgatct gaaggaaata tccccggaga
     6241 aggcagcgga ggtcctggtt tcctggcttg gggaaaaccg cattcgcgtt ctcaacgtgg
     6301 cgggaccgag ggcgggaagg gatccagcca tctatccggc cgtgatggat cttctggagc
     6361 gatcgataga aaacgctggg ccggtggacg tcgttctgtt tgatttcggc ggcgtgctgg
     6421 cggaggaagg ctggaaagac ggtttgcagg cgattgccca cgccaacgga ttgaaagccg
     6481 ttgaacttct cctgattgcg gccgacacgg tgtatgagac aggctacatt ctgggcaaag
     6541 gctccgaaat cagctactgg gatgccatgc gccgcaagac cggcattcgg ggggattatg
     6601 cgcagttgcg ggatgaaatt ctgtcgcgtt tcatccttcg ggagaatatg atcgatcttg
     6661 tcaaaagact gaaggcggac aatcttgtcg tcggtatttt gagcgatcag acggacatgc
     6721 tggaccaact gaacgcccgg atgaattttt ttcagtggtt tgattacgtc ttcaacagct
     6781 accacatggg caagggcaag cgggacgcaa cgttgtttga cgatattgcc caacggctga
     6841 aaaccctgcc ggggcggatt ctattcattg acgatgatcc ggggaacgtt gaacgggcga
     6901 agcagagggg ctggaaggct atcctttact ccgatgaaaa tgtttttcag tcggaattgg
     6961 accgtctgct gagcgcttga ggaccgagaa aggctgttca ggaaaaaggc gaaagatacc
     7021 cgtccggacg ggacaaagcc aatatcgggt ccctgttttt gacggaataa aaatccattc
     7081 cctgcgccgc caaacgaaaa agccccccgg ggatttcccg gagggctttg tgtatcttca
     7141 acagatc
//
LOCUS       DAOPAF010000076         7129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9441_length_7129_cov_60.709782, whole genome shotgun sequence.
ACCESSION   DAOPAF010000076 DAOPAF010000000
VERSION     DAOPAF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9441_length_7129_cov_60.709782"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLQ77_06990"
     CDS             <1..227
                     /locus_tag="PLQ77_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10458.1"
                     /translation="ADYKNTAGRMGGAITGAAFLSKFVGESPWVHLDIAGPSWTTKDR
                     GYIPRGATGVPVRLLVEYLRQYSARKQHVH"
     gene            333..1328
                     /locus_tag="PLQ77_06995"
     CDS             333..1328
                     /locus_tag="PLQ77_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10459.1"
                     /translation="MTMPLLEIQNVKTVFTTQRGVLRAVDDVSLSLSAGETLGVVGES
                     GCGKTMLALSIMRLIPPNGRIAEGRIYFNGQDLLKLSEEEMREKRGRDIAMIFQEPMT
                     SLNPVLRVGEQIAEAIRLHQHVSDREALDRSVKLLGEVGIPDPGRRVKDYPHQMSGGM
                     RQRVMIAMAMSCRPRLLLADEPTTALDVTIQAQILKLISDLKEKNNMAVIWITHDLGI
                     VAEAAQKVAVMYAGKIVEIADVERLFADPLHPYTRGLIESRPSGWAESDAEKGSYLKT
                     IPGTVPGLLDLPPGCRFADRCSLAEERCRAAGPALAELEAGHFVSCFVAESLAKS"
     gene            1350..2318
                     /locus_tag="PLQ77_07000"
     CDS             1350..2318
                     /locus_tag="PLQ77_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL10460.1"
                     /translation="MASILMDIQQLKKTYVLGGGFLGQKPRVIRAVNGVDLQIFEGET
                     LGLVGESGCGKSTMARLLIRLEKPDEGSVSFRGRDIISLASEDVKTYRRRVQMIFQDP
                     YSSLNPRKSVQSIISEPLTIFRAADRTKRKEKVLQLMKKVGLSEEQARRYPHEFSGGQ
                     RQRIGIARALALQPELVIADEPVSALDASIQAQILNLLSDLKKDFGLTYLFISHDLNV
                     IHHVSDRIAVMYLGRIVELADKNDLYREPLHPYTRMLLAAMPGGDPQRKKWGTQVRGE
                     AAEARQAGCSFKNRCIYKIAVCDETVPELKPYPGRGLCACHRTGEI"
     gene            2666..3286
                     /locus_tag="PLQ77_07005"
     CDS             2666..3286
                     /locus_tag="PLQ77_07005"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPL10461.1"
                     /translation="MHHEEKVFWSNGSLTGEGLWHSGAVDAGAVICHPHPLMGGSMHN
                     NVVETVRNVWSAAGFSTLRFNFRGVGKSTGRYDEGRGEKQDLLSACAYAKSRGVKKLF
                     LIGYSFGAWICCRLLRDNPSVATSVLLVSPPLQYFEFDWDGLENTVELMICGDQDTFC
                     DVETLSKKAESISAKLTILSGADHFYFGHEARLAEDLKRYIHEKKA"
     gene            3512..3712
                     /locus_tag="PLQ77_07010"
     CDS             3512..3712
                     /locus_tag="PLQ77_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPL10462.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAIAGTGFKTLYEG
                     QRIRFDIEQGNKGPSAANVQPL"
     gene            3776..4699
                     /gene="miaA"
                     /locus_tag="PLQ77_07015"
     CDS             3776..4699
                     /gene="miaA"
                     /locus_tag="PLQ77_07015"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPL10463.1"
                     /translation="MKKNLIVILGPTASGKTSLAVRLAAELGGEIISADSRQVYRGMD
                     IGTGKDLSDFHLEGRVIDCHLIDILDPDQEFNVFEFQKRFYEIFSTLRKKQVLPLLVG
                     GTGLYLESVLTRYFMPNAEPDARLREELAGLAMEELQDILLSMKPQLHNQTDLQDRER
                     LIRKIEIEKARQNPSNHPDRFPQIHAAVFGVYWERAELKKRISARLLQRLEEGMIEEV
                     SRLHDEGVSWGRLESFGLEYRFIARCLQKKITREEMAAGLETAIGQFAKRQMTWFRRM
                     EKKGVSIEWIPGGDYSALRGRVLDALQRYSP"
     gene            4696..6051
                     /locus_tag="PLQ77_07020"
     CDS             4696..6051
                     /locus_tag="PLQ77_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10464.1"
                     /translation="MKKASLINDYLSRYAAGPSWRLRAEDSGSVEQVVVIPAYAERDL
                     IFFTLASLAANPEAMLEKTMVLCVVNNKADSPKEDRENNALTLALLDALAKRRRLDMP
                     DADGNLQEAWQRVASSRLRLGFVDASSPGLEIPRRAGGVGMARKIGMDAALGLLANSK
                     QKLRLILSLDADTLVKPDYLTGVRTAFSSGRAQAGMVDYEHQMPSDRESQEAICRYEI
                     FLRYWVLGLKFAKSPYAFHSIGSTMAATDEGYLAVRGMNRREAGEDFYFLNKLAKAGP
                     LRRIHETRVYPSARLSNRVPFGTGAAVAKLVSASSGDFGLYDPRVFAVLERWLTLMKE
                     SFEGTANEILAQAKEIHSGLGEFLDGRKFLSVRPKIRGNVKDRRAYERQMHGWFDGFE
                     TLKLINYLTLYFYPKVSLPFAVKKLLEMQNPACPSPFSAEGATGLEEGMRMLHALRRL
                     T"
     gene            6156..>7129
                     /locus_tag="PLQ77_07025"
     CDS             6156..>7129
                     /locus_tag="PLQ77_07025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL10465.1"
                     /translation="MNKFWFKSVLMIFVLALVVPMLACSKEEGTANTTAPAASQSTAS
                     ESILVPEKPAEEPAAPAPASVQALAADAAVSVDGIVLKKSELDKKVQAQMKLYQDKIP
                     ADRKNEVRSGLKKQLTEDFVMRTLLANEVKRRNITATDKEIQSAMDQIKENLPPDKNL
                     KEFLKENNVSRDDIDLGVRIQKLAAMETGGKIKPSEKEISKFYADNKDKFTTGESVHV
                     RHILVAIDARDDEKIKAEKKAKIEGLRKQVLAGADFAEVAKKNSDCPSKENGGDLGEI
                     KRGQTVKPFEDAAFSQEIKTVGPVVSTEFGHHVLQVLGRTPAKTVGLD"
BASE COUNT         1710 a         1780 c         2109 g         1530 t
ORIGIN      
        1 tcgccgatta taaaaacacg gcgggacgaa tggggggcgc gattacgggc gcggcattcc
       61 tcagcaagtt tgtgggcgaa tccccgtggg tgcatctgga tatcgcgggg ccgtcctgga
      121 ccacgaaaga ccggggctac attcccaggg gcgcgacggg tgttcccgtc cgtctgctgg
      181 tggaatactt aagacaatat tctgcaagga agcaacacgt acattgatgt ccgcgatgac
      241 cggcgggttt aaaaacccgc cctgcgtcgt tgctgtgaaa aaaactaatg tttctgtagg
      301 gcgggccttc aggcccgccg ttaccgggtc aaatgacgat gccgcttctg gaaattcaaa
      361 atgtaaaaac cgtttttaca acgcagcgcg gtgttttgcg ggccgttgac gatgtttccc
      421 tgtccctttc cgccggagaa acgctgggcg tcgtgggcga atccggctgc ggcaaaacca
      481 tgctggctct ttccatcatg cggctgattc cgcccaatgg ccggattgcc gaaggccgga
      541 tttacttcaa cggccaggat cttcttaagc tttcggaaga agaaatgcgc gaaaagcgcg
      601 gccgggacat tgccatgata tttcaggagc cgatgacgtc cctcaatcct gttttgcgcg
      661 taggcgagca gatcgcggag gccattcgcc tgcaccagca tgtttcggac cgggaggcgc
      721 tggaccgcag cgtgaaactg ctgggcgagg tcggcattcc cgatccggga agaagggtga
      781 aggattatcc gcatcagatg agcggcggca tgcgtcaacg ggtcatgatt gccatggcca
      841 tgtcctgccg cccccggttg ctgctggcgg acgaacccac cacggcgctg gacgtgacta
      901 ttcaggcgca gattctgaag ctgatttccg atctcaagga aaaaaacaac atggcggtta
      961 tttggatcac gcacgatctg ggaatcgttg ccgaggcggc ccagaaggtg gcggtcatgt
     1021 acgcgggcaa gatcgtggaa attgcggacg tggaaagatt gtttgccgat ccgctgcacc
     1081 cctacacgcg cgggctgatc gaatcacggc cctcgggatg ggcagaaagc gatgcggaaa
     1141 aggggtccta tttaaaaacc attccgggaa cggttcccgg cctgctggat ctgccccccg
     1201 gctgccgttt tgccgaccgc tgctcgcttg ccgaagagag gtgccgggcg gccgggcctg
     1261 cgcttgcgga attggaagcg gggcattttg tctcctgctt cgtcgccgag agccttgcga
     1321 aaagttaatg acgcattgtc gaggtgttca tggcttccat attgatggat attcagcaac
     1381 tgaaaaaaac ctacgtttta ggcggcgggt ttctggggca gaagcccagg gtcattcggg
     1441 cggtgaacgg cgtcgatctt cagatcttcg agggagaaac gctggggctc gtcggcgagt
     1501 cggggtgcgg caaatccacg atggcgcgcc ttctgatcag gctggagaag ccggacgagg
     1561 ggagtgtttc tttcagaggc cgggatatta tttccttggc atcggaggat gtaaagacgt
     1621 accggcggcg cgtacagatg atttttcagg atccttattc ttcgttgaac ccgcggaaat
     1681 ccgtccaaag catcatttcc gagccgctca caattttccg ggcggccgat cgaactaagc
     1741 ggaaagaaaa agtgcttcaa ctgatgaaga aggttggcct cagcgaagaa caggcccgac
     1801 gctatcccca cgagttcagc ggaggacaga ggcagcgcat cggaatcgcc agggcgctgg
     1861 cgctgcagcc ggagctggtc atcgcggacg agccggtttc cgcgctcgat gcttccatcc
     1921 aggcgcagat tctgaatttg cttagcgatc tgaaaaaaga tttcgggctg acctatcttt
     1981 ttatctccca tgacctcaat gtcattcatc atgtatcgga caggattgcc gtgatgtacc
     2041 tgggccgcat cgttgagctg gcggacaaga atgatctcta ccgggagccg cttcacccct
     2101 acacccgaat gctgctggcg gccatgccgg gcggtgatcc gcaaaggaaa aaatggggaa
     2161 cgcaggttcg cggagaggcg gccgaagccc ggcaggcggg ttgcagtttt aaaaaccgct
     2221 gcatttataa aattgccgtt tgcgatgaga cggttcccga actgaagccc tatccgggaa
     2281 ggggcctgtg cgcctgtcac cgcacgggcg agatatgatc ccaaaccgtt tgtgatttta
     2341 tagaagagac aatgcgctgt cattgcgagc aatttaccag gagctacgca ccaggttata
     2401 aatggaagaa actgtaatct cccctccctc gatgggaggg gagggggagg gtgaaaacaa
     2461 aggcttcccc ctcccctcaa tccccgccca ccaggggcgg ggaagtatct taggagcaga
     2521 ctcttggtct gcgtggcaat ctaaaaaagg agattgcttc actttgctcg caatgaccag
     2581 ttcttgttat cgtgagaata caaaacttaa cctgcgtgcc acctggttat ggcgtaatct
     2641 taaatattca aatggagcaa tgaccatgca tcatgaagaa aaagttttct ggtccaacgg
     2701 cagtctcacc ggcgaaggtt tgtggcattc gggcgcagtg gatgcgggcg cggtgatctg
     2761 ccatccgcat ccgctgatgg gcgggtccat gcacaacaat gtcgtggaaa ccgtccgcaa
     2821 cgtatggagc gcagccggct tttccacgct gcgctttaac ttccgcggcg tcgggaaaag
     2881 caccggccgc tatgacgagg gcaggggaga aaagcaggat cttttgtccg cctgcgcata
     2941 cgcaaaaagc cggggcgtta aaaaattatt tctgatcggt tattcgttcg gcgcgtggat
     3001 ctgctgtcgc ctgctcaggg acaatccctc cgtcgcgacg tccgttttgc tggtatcccc
     3061 tccattgcaa tacttcgagt tcgactggga cggactggaa aacaccgtgg aattgatgat
     3121 ctgcggggat caggacacgt tctgcgatgt cgagaccctc tcaaaaaaag cagaatccat
     3181 ttcggcaaaa ttgacgatcc tttccggagc cgatcatttt tatttcggac acgaagcccg
     3241 gctggctgag gatttgaagc ggtatattca tgaaaaaaaa gcttgatttt aaataaattg
     3301 gtgttatgtt ctgatcaaat gcatcggttt tagagccggt tttacaaggg ttggaagctt
     3361 cgggtcggta aataagtagt gttgagattg atttacaaaa aaagaaaata accgcaaaga
     3421 gttctttttg aaataagaat tcttgcggtt tttttagttt ctgacgggat gaaccggaag
     3481 aaacaaaaat ttgaaagggg gggctttaga agtggcagaa ggtaaagtga agtggtttaa
     3541 tgagaagaag ggttttggct tcattgaaaa tgatgaaggc ggagatgttt ttgtacatta
     3601 tagcgccatt gcgggaacgg gtttcaagac tctgtacgag ggtcagagaa ttcgttttga
     3661 cattgagcag ggcaacaaag ggcccagcgc ggcgaatgtt cagccactct agtcgctgaa
     3721 aacgatgaac aaaaaaatgc cccgtcttga ggggcatttt ttttagctga aaatcatgaa
     3781 aaaaaatctg atcgtcatcc tgggcccgac ggcttcgggc aaaacgtcgc tggccgtccg
     3841 tctggccgcc gaactcgggg gagaaatcat ttccgcggat tcgcgccagg tttatcgcgg
     3901 gatggatatc ggcacaggca aggatctgag cgactttcat ttggagggcc gcgtcattga
     3961 ctgccatctg atcgacattt tggacccgga tcaggagttc aatgttttcg agtttcagaa
     4021 acgcttttac gaaatatttt ccacgctcag gaaaaaacaa gtcctgcccc ttctggtcgg
     4081 cggcacaggc ctgtacctgg aatcggtcct gacccgatac ttcatgccga atgccgaacc
     4141 cgacgcgcgc ctgcgggaag agctggcggg gctggcgatg gaagagcttc aggatatttt
     4201 gttgtccatg aaaccccaac tgcacaatca aaccgatctt caagaccggg aacggctgat
     4261 ccggaaaata gaaatcgaaa aagccaggca aaacccgtca aaccacccgg accggttccc
     4321 gcaaatccat gccgctgtct tcggtgttta ttgggagcgc gccgaactga aaaaaagaat
     4381 ctcggcgagg cttctccagc ggctggagga ggggatgatt gaagaagtgt cccggctgca
     4441 cgacgaggga gtgtcctggg gaaggctgga gagctttggt ctggaatacc ggttcatcgc
     4501 ccggtgcctg caaaagaaaa taaccaggga agagatggcg gccggcctag aaaccgccat
     4561 cgggcagttt gccaagcgcc agatgacatg gtttcgccgc atggaaaaaa agggcgtttc
     4621 gattgagtgg attcccggcg gcgattattc cgcgctgcgc ggccgcgtcc tggacgcgct
     4681 tcagagatat tctccgtgaa gaaagcttcc ctgatcaatg attatctgag ccggtacgcg
     4741 gcggggccgt catggcgtct gcgggcggag gattccgggt ccgtcgaaca ggttgtcgtc
     4801 attccggctt atgcggaaag agacttgatc tttttcacgc tggcttctct cgccgccaat
     4861 cccgaggcga tgctggaaaa aacaatggtg ctgtgcgtgg tcaacaacaa agccgactcg
     4921 cccaaagaag acagggaaaa caacgctcta acccttgcgc ttctcgatgc gctggcgaaa
     4981 aggcggcgtt tggatatgcc ggatgcggac ggaaatcttc aggaggcatg gcaaagagtc
     5041 gcctccagcc gtttgcggct gggatttgtg gatgcttctt ctcccggact ggaaattccc
     5101 cgccgcgcgg gcggcgtcgg aatggcgcgc aagatcggca tggacgcagc tctcggcctg
     5161 ctggcaaatt cgaaacaaaa gctccgtttg attctgagcc ttgacgcgga tacgctggtc
     5221 aagccggatt atctgacagg cgtccgcaca gctttttcct ccggccgggc gcaggccggc
     5281 atggtggatt acgaacatca aatgccttcg gacagggaga gtcaggaggc gatttgccgc
     5341 tatgagatct tccttcgcta ctgggttctg ggtctcaaat ttgcaaaatc accctacgcg
     5401 tttcattcca tcgggtccac gatggcggca acggatgaag gatacctcgc cgtgcggggc
     5461 atgaatcgcc gcgaggcggg cgaggatttt tattttctga acaagctggc caaggcgggc
     5521 cccctgcgca gaattcacga aacgcgagtt tatccttccg cgcggctttc aaatcgcgtt
     5581 cccttcggga cgggcgcggc ggtggccaaa ctggtttccg cctcgtccgg tgacttcggg
     5641 ctgtatgatc cgcgcgtctt tgccgttctt gaaaggtggc tcacactgat gaaagaatct
     5701 tttgaaggca cggcgaatga gattctcgca caagcgaaag agattcattc gggccttggg
     5761 gaatttttgg acggacggaa atttttgtcg gtccggccga aaattcgcgg caacgtgaag
     5821 gatcgcaggg cctatgaaag acagatgcac ggctggtttg acggcttcga aacattgaag
     5881 ctgatcaatt atctgactct gtatttttat ccgaaagttt ccctgccttt cgcggtgaag
     5941 aagcttctgg agatgcaaaa cccggcatgc ccgtccccgt tctccgccga aggagcgacc
     6001 ggccttgaag agggcatgag gatgctccat gcccttcggc ggctgacctg aaagcgggaa
     6061 ttcgacaacg ccggggataa tcttcttgac tttgcaaaag aacttcgcgt atagcgcaaa
     6121 aacatgacgc ctgcagaggg tccgggagga aattcatgaa taaattctgg ttcaaaagcg
     6181 ttctgatgat tttcgtgctg gcactggttg taccgatgct tgcctgcagc aaagaggagg
     6241 ggacggcaaa cacaaccgca ccggctgctt cgcaaagcac cgcttccgaa tcgatcctgg
     6301 tgccggaaaa acccgccgaa gagccggcgg cgcctgcacc cgcttccgtt caggcgcttg
     6361 ccgccgacgc ggccgtcagt gttgacggca ttgtcctgaa gaagagcgag cttgacaaaa
     6421 aagttcaggc ccagatgaaa ctgtatcagg acaagatacc ggccgaccgg aaaaacgaag
     6481 tgcggtccgg tttgaaaaaa cagttgacgg aagattttgt gatgcggacg cttttggcca
     6541 atgaagtcaa acgcagaaac atcacggcca cggacaagga aatccagtcg gccatggatc
     6601 agatcaaaga aaatcttcca cccgataaaa accttaagga atttcttaag gaaaataacg
     6661 tatcccgcga tgacattgat ctgggtgttc gcatacaaaa actggcggcc atggaaaccg
     6721 gcggaaaaat caagccgtcg gaaaaggaaa tcagcaagtt ttacgcggac aacaaagata
     6781 agttcaccac cggggaaagc gttcacgttc ggcatatcct ggtggccata gacgccaggg
     6841 acgacgagaa gatcaaggct gaaaagaagg caaagataga aggcctgcgc aagcaggtcc
     6901 tggccggcgc cgatttcgcc gaagtggcca aaaaaaattc cgactgcccc agcaaggaaa
     6961 acggcgggga tctgggcgaa atcaagaggg ggcagacggt gaagcccttt gaggacgcgg
     7021 cgttttcgca ggagatcaaa acggtcggac cggtggtgag cacggaattc ggccatcacg
     7081 ttcttcaggt gctggggcgc acaccggcca aaaccgtcgg tctggacga
//
LOCUS       DAOPAF010000077         7012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9640_length_7012_cov_52.779359, whole genome shotgun sequence.
ACCESSION   DAOPAF010000077 DAOPAF010000000
VERSION     DAOPAF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9640_length_7012_cov_52.779359"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..376)
                     /gene="rplU"
                     /locus_tag="PLQ77_07030"
     CDS             complement(65..376)
                     /gene="rplU"
                     /locus_tag="PLQ77_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPL10466.1"
                     /translation="MYAIIKTGAKQHKVSEGDVLSVEKLPGDKGAEVVFQEVLMVSDE
                     AGVKIGRPFVEGATVIGEVVSQTKGPKLLIGKYKKRKGFRKKTGHRQELTSMKIKKIS
                     I"
     gene            586..1482
                     /locus_tag="PLQ77_07035"
     CDS             586..1482
                     /locus_tag="PLQ77_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPL10467.1"
                     /translation="MNRRGALLVFIVFFLAWPALAQAAGDDPRAAVEELLVAEGLDPA
                     KVRSLLQDSRVSVRPEIVLKNLFFSKPKGSGKDPDVMEIDPRQIERGREFMKENDAVL
                     SDVERRFGTSPRIITAILIIESRLGTYPMPYNVVNAYANLAFMADPAYWEEIQSRYGD
                     AYPEIRSDAAAARAKQKAKWAVRELYHLSQIADHLGIDPLSLSGSFAGAMGPAQFIPS
                     SFWVFGMDGNGDGLADPSNMTDANFSMGHYLRKFGWAENASVEQKRRAIWHYNRSRVY
                     VNTVMMLYEELGRKNHQESTNE"
     gene            complement(1458..3098)
                     /locus_tag="PLQ77_07040"
     CDS             complement(1458..3098)
                     /locus_tag="PLQ77_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPL10468.1"
                     /translation="MEKENPKNESAPAKKTPDEKQKKEKAPLRPELAELIKRLSFGLD
                     ENRPDAVEKRKKKNQRTARANIEAFCDPGSFVEYGALAVAAQRGRRSVDDLIAKTPAD
                     GLIAGVAAVNGAHFPEDKARCMIMAYDYTVLAGTQGYFNHKKMDRMLELAYEQKLPLV
                     FFGEGGGGRPGDVDAMTVMVAGLDLGTFGSFARLSGRVPVVGIVSGPCFAGNAALLGC
                     CDVIISTKNSNIGMGGPVMIEGGGLGVFKPEEVGPMDVQTQNGVVDIEVADDIEAVAA
                     AKKYISFFQGPLPAWTAGDPLKLRDVIPESRKRAYNVRSVIKAIADTDSFIELRPRFG
                     PGMVTGLLRIEGRPFGVIANNPMHMAGAIEAEGADKAARLMMLCNAHGLPILSLCDTP
                     GFMVGPEIEKRAQVRHVCRMFVVGSHVTVPYFTVILRRGYGLGVMAMARGGFHQSFFS
                     VSWPTGEFGGMGLEGAVKAGFKKELAAIEDLQKREEFYESLVAQLYEIGKAINMAAHL
                     EIDAVIDPADTRRWIMRGLKSAPARQPADAGHSFVDSW"
     gene            3378..4178
                     /locus_tag="PLQ77_07045"
     CDS             3378..4178
                     /locus_tag="PLQ77_07045"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL10469.1"
                     /translation="MDLGIQGKISVVTGGGKGIGEAIALRLASEGAKVVLADLDSEAA
                     KKAADAIQAQGGNALAVPCNTTNPDQVDALMEKTGETYGAVDILVNNVGGGTGIDLVV
                     RSKVEDWDKTVEMNLKTAYLCCRAAAKVMIPRKRGRIINMSSISGKQGEPLLGSYCAA
                     KFGVIGFTQTLARELARYDITVNAVCPGYVFTPGWEKLAQALKDSQPALADKSLLEIF
                     EARVRSVTPLRRPQEASEIAGLVAYLASYEARSITGQAISIDGGIATG"
     gene            complement(4447..5412)
                     /locus_tag="PLQ77_07050"
     CDS             complement(4447..5412)
                     /locus_tag="PLQ77_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPL10470.1"
                     /translation="MKMKEIKASTMNVEKFIDEKVRDIRSAVGDGLAVNALSGGVDSC
                     VVTALGHKALGGRLKTYMIDNGIMRENEPARVQTAFKKLGIHVEVIDASKAFFAALRG
                     LTDPEEKREAITQTFYKNVFGKLVVKSKAKYLLQGTILTDVDETVAGIKRQHNVFEQL
                     GIDPQKAFGYKILEPLIELRKDGVRKAGAGLGLPSAMFDRIPFPGPALAARVIGEVTP
                     ARIALVRKATTITEAALKDTKAFQYLAILHEDRVTGMRDGKRVFGNQIEIRSWESTDA
                     RRASPSRLSFDVLEKIAAKITKEIPEVVSVTYNITTKPTSTIEAI"
     gene            5598..6359
                     /gene="hisF"
                     /locus_tag="PLQ77_07055"
     CDS             5598..6359
                     /gene="hisF"
                     /locus_tag="PLQ77_07055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPL10471.1"
                     /translation="MEPIKIMPCLDMKNGRVVKGVHFVDIKEAGDPVENAAFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKKVSAQISIPLTVGGGISSLEDIEMVLGAGASKVSM
                     NSAAVKNPELVRLASAKFGPEKITVAIDAKRNPAMSSGFELVVSGGTLPIAKDAVAWA
                     RQCQELGAGVILPTSMDGDGTKAGYDIPFTKAISSAVSLPVVASGGAGKLEDFYEAVV
                     DGGASVLLAASVFHFRLLKIKDVKDFLKSRGLAVA"
     gene            6397..>7012
                     /locus_tag="PLQ77_07060"
     CDS             6397..>7012
                     /locus_tag="PLQ77_07060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL10472.1"
                     /translation="MSKLADIIFFRQHGICPRWFCFTFDNGLRRLVQNPDRIIRPYIG
                     EGNKVLDVGPGIGYFTIPIARRAGSGGKVIAADIQESMLRGIAKRAERAGVSGQVELY
                     LSQPDEIYSGEPVDFILSFWMAHEVPRLDRFFRQLRAALKPAARFLLAEPALHVSRAQ
                     FDGEIDLAQKAGLVLLDRPAISLSHGALFGSSPAVAASESVSADR"
BASE COUNT         1440 a         1928 c         2072 g         1572 t
ORIGIN      
        1 aattggagtc tctaccgttg cgggagcttc cctgaccttt tttatgtgcc atgatgtcct
       61 ccgcctatat cgaaatcttc ttgattttca tgctggtcaa ttcctgacga tgaccggtct
      121 tctttcgaaa acccttgcgt tttttatatt tgccgatgag cagcttcggt cctttggtct
      181 gggagaccac ttcaccgata acggtggcgc cttcaacgaa gggcctgccg atttttacac
      241 cggcctcgtc cgacaccatc aaaacttcct gaaaaacaac ttcggcgccc ttgtcaccgg
      301 gcagtttttc aacggataat acatcgccct cgctgacttt gtgctgtttg gctcctgttt
      361 ttataatcgc gtacatatcc ttatcctttg ctgaagtaag aacgaagctt atagacaaaa
      421 tgaacctttt tgtcaataaa attcgccagc cctcgcgata ttttatccgg ccgcccgcgg
      481 gcatggctaa aatgccgata ttaagaagaa aatcgcttgc attccacggc ggccaaaagg
      541 taggatcaac ggcaggcggc aggcagaaac gatcgaggtg tatttatgaa cagacgcggc
      601 gctcttctcg ttttcattgt tttttttctg gcctggcctg cgcttgcgca agcggcaggc
      661 gatgacccgc gggcggcagt cgaggaactg ctggtcgccg aagggcttga tcccgcgaag
      721 gttcgcagtc tcctgcagga cagccgtgtg tccgttcgcc cggagattgt gctgaaaaac
      781 cttttctttt caaagcccaa agggtccgga aaggatcccg atgttatgga aatcgaccct
      841 cgtcagatcg aacggggaag agaattcatg aaggaaaacg acgctgtttt gtccgacgtc
      901 gagcggcgct tcgggacgtc gccgaggatc atcacggcga ttctgatcat tgaatcccgg
      961 ctgggaacct atcccatgcc gtacaacgtg gtcaacgcct acgccaatct ggcctttatg
     1021 gcggatccgg cctactggga ggaaatccag agccgctacg gggacgctta tcccgagatt
     1081 cgaagcgacg cggccgccgc ccgggcgaaa caaaaggcaa agtgggcggt gcgggagctg
     1141 tatcacttaa gccagatcgc cgatcatctt ggaatcgatc cattgagcct atcgggatct
     1201 tttgccggcg cgatgggccc ggcgcagttt attccatcct ccttctgggt ttttggaatg
     1261 gacggcaacg gggacggatt ggccgatccc tccaatatga cggatgccaa tttcagcatg
     1321 gggcattatc tcaggaaatt cggctgggcg gagaatgcct ccgttgaaca aaaacgaagg
     1381 gcaatctggc actacaaccg cagccgcgtg tacgtcaata ccgtgatgat gctctacgaa
     1441 gaactgggac ggaaaaatca ccaggaatcg acaaatgaat gacctgcatc cgccggctgt
     1501 cgggcgggcg cggatttcag accccgcatg atccaccggc gggtgtcggc cggatcgatg
     1561 acggcgtcga tttccaggtg cgcggccatg ttgatggctt tgccgatttc gtagagctgc
     1621 gccaccagcg attcatagaa ctcttctctt ttttgcaggt cctcaatggc cgccagttct
     1681 tttttgaagc ctgctttgac ggcgccttcc agtcccattc cgccgaactc cccggtcggc
     1741 caggacacgc tgaagaatga ttgatgaaat ccgcccctgg ccatcgccat gacgccgagt
     1801 ccgtagcccc ggcgcaggat gaccgtgaag taaggcacgg tgacatgcga gccgacgaca
     1861 aacatccggc agacatggcg gacctgcgcc cgtttttcaa tttcgggacc gaccatgaag
     1921 cccggcgtgt cgcacagcga cagaatcggc agtccgtgcg cgttgcacag catcatgagg
     1981 cgcgcggcct tgtccgcgcc ctcggcttca atggcgcccg ccatgtgcat ggggttgttc
     2041 gcgatcacgc cgaacggacg cccttcaatc cgcagcagcc ccgtgaccat gccggggccg
     2101 aagcgggggc gcagttcgat gaaggaatcc gtatccgcga tcgccttgat gacgctgcgc
     2161 acattatagg cgcgcttgcg gctttcggga atcacgtcgc gaagtttgag cggatcgccc
     2221 gccgtccacg cggggagggg accctgaaag aaagagatat actttttggc cgcggcgacg
     2281 gcttcgatgt cgtccgccac ttcaatatcc actacgccgt tttgggtttg cacgtccatc
     2341 gggccgactt cctcgggttt gaagaccccc agcccgccgc cttcgatcat caccggaccg
     2401 cccatgccga tattggaatt cttcgtggag atgatgacat cgcagcagcc cagcagcgcg
     2461 gcatttcccg cgaagcaggg gccggaaaca atgccgacga ccggaacacg tccgctgagc
     2521 cgtgcgaaag aaccgaaagt gcccagatcc agccccgcga ccatcacggt catcgcgtcc
     2581 acgtcgccgg gtcttcctcc tcccccttcg ccgaaaaaga ccagcggcag cttttgttca
     2641 taggccagtt ccagcatgcg gtccattttc ttgtggttga aatatccctg cgttccggca
     2701 agcaccgtgt agtcgtaggc catgatcatg cagcgggcct tgtcctccgg gaaatgcgct
     2761 ccgttgacgg cggcgactcc ggcgatcaga ccgtcggcgg gcgttttggc gatcaggtcg
     2821 tccacggagc ggcgcccccg ctgcgcggcc acggccagcg ctccgtattc gacaaagctt
     2881 cccggatcgc aaaaggcctc gatgttggcg cgggcggtcc gctgattttt cttttttctt
     2941 ttttccaccg cgtcagggcg gttttcatcc aggccgaacg aaagtcgctt gatcagttcg
     3001 gccagttccg gccgcaaagg ggccttttcc tttttctgtt tttcatccgg cgtttttttt
     3061 gccggggccg attcgttttt cgggttttct ttttccatgc gcttcagact cccccattga
     3121 ctattgattc cggcatgatg ccgtccggtt cggcggttgt tcttccggcc ccgcgccggg
     3181 acgtcgatct taccatgaac cggttttttt aacctgccgc cttcgaatag ctaaaataaa
     3241 atttgatgtc aatggaaata gccgcatcgc ggcgggatgg ggcggggtga gcctgaattc
     3301 gtcattaaaa tatcaacatg gacaaaacca tttttttgcc gtatttcaga cgtaaatcat
     3361 ttcagggggg cgaaacaatg gacctgggca ttcaagggaa gatcagtgtt gtcacgggcg
     3421 gaggcaaggg catcggcgaa gcgatcgctt tgcgcctggc ttccgaagga gcgaaggtgg
     3481 ttttggcgga tctggattcg gaggcggcaa aaaaagctgc cgacgcgatt caggcgcagg
     3541 gagggaatgc gctggccgtc ccctgcaata ccacgaatcc ggaccaggtc gatgccctca
     3601 tggagaaaac cggagagact tacggcgctg tcgatattct ggtcaacaac gtgggcggag
     3661 gcacggggat tgacctggtg gtccggtcga aggttgagga ctgggacaag acggtggaaa
     3721 tgaatctgaa gaccgcctat ctctgctgcc gggccgccgc caaagtcatg attcccagaa
     3781 aacggggacg aatcatcaat atgtcctcca tttcaggaaa acagggagag ccgcttctgg
     3841 gttcgtactg tgccgcaaaa ttcggcgtca tcggatttac gcaaaccctg gccagggagc
     3901 tggcgcgcta cgacattacg gtcaatgccg tgtgccccgg ctatgttttc acgccgggct
     3961 gggaaaagct ggcgcaggcg ctcaaggatt cgcagccggc cctggcggac aaaagcctgc
     4021 tggaaatctt tgaagcgcgt gtccgatccg taacccctct gcgccgtcct caggaggcct
     4081 ctgaaatcgc cgggctggtg gcctatctgg cgtcttacga agcccgcagc atcacaggcc
     4141 aggcgatcag catcgacggc ggcatcgcga cggggtagac catacggctt ctcatccgcc
     4201 cgggccgtct gcggattatg gcgtccgtcg ggactattac gaacggcaag ttcgacaagt
     4261 cggagcttta tgtttggcgg ggggatcaga aagaatacga gcgcgtcaaa aaactgtcca
     4321 cggcaaccaa caaccccatt atctttctga aacgatagga acgaaaagtc ttgaagataa
     4381 cgggaagggg cagggaaacc tgccccttct tttttgaaat ttgcggcaag gccgtgttcc
     4441 ccagcgctaa atggcctcga tggtggaggt cggtttggtc gtgatgttgt aggtgacgct
     4501 cacgacttcc ggaatctctt tcgtaatttt tgcggcgatc ttttccagta cgtcaaagga
     4561 cagccgggaa gggcttgccc tgcgcgcgtc cgtgctttcc caggagcgga tttcgatctg
     4621 attgccgaag acgcgcttgc cgtcgcgcat tcccgtgacg cggtcttcat gcagaatcgc
     4681 caggtactgg aaggctttcg tgtctttgag cgccgcttcg gtgatggtcg tggcttttct
     4741 caccagcgca atcctggcgg gtgtgacctc cccgatcacg cgggcggcca gcgccggccc
     4801 cgggaaggga atccggtcga acatggcgga aggcaggccc agccccgcgc cggctttgcg
     4861 aactccgtcc ttgcgcagct cgatgagcgg ctccagaatt ttatagccga acgctttttg
     4921 cgggtcgatg cccagctgtt caaagacgtt gtgctggcgc ttgatgccgg cgacggtttc
     4981 atccacgtcg gtaagaatgg tgccctgcag aagatatttg gccttgcttt tgacgacgag
     5041 tttgccgaaa acatttttgt aaaaagtctg cgtgatggct tccctttttt cttcgggatc
     5101 ggtgaggccc ctgagggcgg caaagaaggc tttggaggca tcgatgactt ccacatggat
     5161 gcccagtttc ttgaaggccg tctgcacccg ggccggctca ttttcccgca tgatgccgtt
     5221 gtcgatcatg taggttttga ggcggccgcc cagcgccttg tgtcccaggg cggtgacgac
     5281 gcaggagtcc acgccgcccg agagcgcatt gacggcgagg ccgtcgccca cggcgctgcg
     5341 gatgtcccgg accttttcgt cgataaattt ttccacattc atggtgctgg ctttgatttc
     5401 cttcattttc attttggcgg ctcctttgcg gtaaaatagg ttgcaggttt gtttaaatga
     5461 aaaccccccg cgtttcaagc attaagatta aaaatatcaa aaacatttcg attgtaaatt
     5521 tgggaagttc ggtttagaaa gcaggccgtt tgaagggcaa agccggcgca aaggcttccc
     5581 ccggaaagga gagtggaatg gaaccgatca aaatcatgcc ctgcctggat atgaaaaacg
     5641 gaagggtggt aaaaggagtt cattttgtcg acatcaaaga ggcgggcgat ccggtcgaga
     5701 acgccgcatt ctatcaggcc gaaggcgcgg atgaactggc gatgctggac atcgccgcca
     5761 cactcgaaaa ccgcaaaaca aggctggaat gggtgaagaa ggtttccgcc cagatcagca
     5821 ttccgctgac cgtcggcggc ggcatttcct cgctggagga cattgaaatg gtgctgggcg
     5881 cgggcgcgag caaagtgtcc atgaacagcg cggcggtgaa aaatcccgaa ctggtcaggc
     5941 tggcgtcggc aaaattcggt ccggaaaaaa tcaccgtagc catcgacgcc aagagaaatc
     6001 cggcgatgtc gtcgggcttt gaactggtgg tttccggcgg cacgctgccc atcgcgaaag
     6061 acgcggtggc gtgggccagg caatgccagg aactgggcgc gggcgtgatc ctgcccacca
     6121 gcatggacgg cgacggcaca aaggcgggat acgatattcc cttcaccaaa gcgatttcct
     6181 ccgccgtcag cctgccggtg gtggcgtccg gcggcgcggg aaagctggag gatttttacg
     6241 aagcggttgt ggacggaggc gcgtccgttc tgctggcggc gtccgttttt catttccggc
     6301 ttctcaaaat taaggacgtc aaagactttc tgaaaagcag gggacttgcc gttgcctgaa
     6361 atccgggtag cgggacagaa aagattagga ggcgcagtga gcaaactggc cgatattatt
     6421 ttttttcgac agcacgggat ttgtccccgc tggttctgct tcacgtttga caatgggctg
     6481 cgccgactgg ttcaaaatcc ggaccgcatc atccgtcctt acatcgggga agggaacaag
     6541 gtgctggatg tggggccggg catcgggtat tttacgattc cgattgcccg gcgtgcaggc
     6601 tcgggcggga aggttatcgc cgccgatatt caggagtcga tgctgcgggg cattgcaaag
     6661 cgcgccgaac gggcgggggt gagcggccaa gtcgagcttt atctttccca acctgacgaa
     6721 atttattcgg gggagccggt agactttatt ctgtccttct ggatggcgca tgaagttccc
     6781 cggctggacc ggtttttccg tcagttgcga gccgccctca agcccgcggc tcgcttcctt
     6841 ctggccgaac ccgcactgca tgtgtcccgg gcgcagtttg acggggaaat cgatctggcg
     6901 caaaaggccg gcctagtgct cttggatcgt cctgcaattt ctttgagcca cggcgcgttg
     6961 tttggaagca gtccggcggt cgctgcaagt gaaagcgttt ccgcggatcg ga
//
LOCUS       DAOPAF010000078         6934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9745_length_6934_cov_39.542375, whole genome shotgun sequence.
ACCESSION   DAOPAF010000078 DAOPAF010000000
VERSION     DAOPAF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9745_length_6934_cov_39.542375"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLQ77_07065"
     CDS             complement(<1..1397)
                     /locus_tag="PLQ77_07065"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPL10473.1"
                     /translation="MNIDKTILSEENLRKIKALNNPHVERIVEEYAALCKPAKVSVIT
                     DSFADIGYIRQMALDLGEEKKLAMPGHTIHFDSFYDQGRDKKNTCLLVTQDMKVSKII
                     DVKDREEGLTEIRGIMDGIMKGKECFVRFFCLGPLHSRFSIAALQITDSAYVSHSEDL
                     LYRVGYEHFKTLNGSDDFFYLVHSAGELDERGNTKNIKDRRIYIDVIGKKVLTVNNQY
                     AGNSVGLKKLALRLAIYKANKEDWLTEHMFIMGVKPEGRKRVTYFTGAFPSACGKTST
                     AMVPGQQIVGDDIAYIRHGEDGRAYAVNIEQGIFGIIEDVNPIDDPVIYEALTTPREL
                     IFSNVLVKDDKPYWLGMGRILPEEGHNFSGEWKKGKKDEKGNEILLAHKNARYTIRLN
                     ELKNVDPKLYDPEGVPVSGIIYGGRDSDTSVPVYQSFDWAHGMFIGASLESETTAATI
                     GAVGVRELSPMANLD"
     gene            complement(1719..2315)
                     /locus_tag="PLQ77_07070"
     CDS             complement(1719..2315)
                     /locus_tag="PLQ77_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10474.1"
                     /translation="MKKFFFGMIFLALVVLTPISAMADVDVKIGINIPLPPPIIFPAP
                     PEVVVIPETYVYAVPDVDADIFFYGGWWWRPWEGRWYRSRYYDSGWNYYDRVPSFYRG
                     VPPGWKNDYRDRRWKGREWDQRRIPSGDAQKNWRSWKRNKHWEKENHWGVREMDRRPP
                     GQQKKYQDRYDGPPRGGGPGNGPKGGPGKGRPDKGNKR"
     gene            complement(2389..2760)
                     /locus_tag="PLQ77_07075"
     CDS             complement(2389..2760)
                     /locus_tag="PLQ77_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPL10475.1"
                     /translation="MQFTFAHNNFNVLNLEKSLAFYREALGLKEARRYEPPDGSFVLV
                     YLGDGQTAHQLELTWMRERTDPYSLGDNEFHLAFRVDDFAAAHALHEKMGCICFENKK
                     MGIYFINDPDDYWLEIVPAKR"
     gene            complement(2812..3969)
                     /locus_tag="PLQ77_07080"
     CDS             complement(2812..3969)
                     /locus_tag="PLQ77_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPL10476.1"
                     /translation="MNNFTFYNPTKILFGEGRIKDIAEEIPMDAKILMTYGGGSIKRN
                     GVYDEVRAALQGRAMEEFEGIEPNPTYETLMRCVEMARKNKIDFLLAVGGGSVIDGTK
                     FIAAAVPYEGDPWEIVATYGKKAKGAMPFGTVLTIPATGSEMNNGAVITNRALNAKLP
                     FMHPSLFPKFSVLDPAKTFTLPPEQIGNGVVDTFVHVTEQYLTFPVNGKLQDRFAEGL
                     LLTLIEEGPRALAEPENYDVRANLMWCATLGLNGLISVGVPQDWATHMIGHELTALRG
                     LDHGKSLAVVLPVLLKIRAGEKKEKLLQYAERVWGIAAGDEAARIDAAIEKTRDFFES
                     MKVPTRLCAYNIGADVIPEVVENLKAHGMVKLGETRDITPELVESMMKDCL"
     gene            complement(4092..5018)
                     /locus_tag="PLQ77_07085"
     CDS             complement(4092..5018)
                     /locus_tag="PLQ77_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10477.1"
                     /translation="MQTPFRIHDLILIVVVVLTMAVAVIFPDFGSRFQAFPVYCLMVN
                     FFLSYLSIDPADVWKAFRDHIREILGFTVLKLALLPVVLYGVFYLVAPDYALAALLLT
                     GVSTGVVSPMISNMVKGNSSLVLLVVVITSALAPFTLPALVKIVIAKEAAISFWAMLR
                     LLATVIFVPIALVEIIRCLTPRLIAPILKIQFPVSLLMFALINLGVFYRYAPFFQEKP
                     SVIIMATVVVFVLAAIYCAVGIFFFRKGPLHNHLAGAVMLGNINNVLVIVFSSQFFGP
                     VEPLVAAMYMIPFFVIVIPLRYYRYRKIRHRS"
     gene            complement(5083..5466)
                     /locus_tag="PLQ77_07090"
     CDS             complement(5083..5466)
                     /locus_tag="PLQ77_07090"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HPL10478.1"
                     /translation="MYNLLADAVIIVHFLFILFVAAGGLLILRRPRMAWLHLPAACWG
                     AFIEFAGWICPLTPLENHLRRLGGEAPYTGDFVVYYLVPVIYPAGLTPLTQYILGGLV
                     VAVNLVIYGYVLYRRFLRRAQASDQ"
     gene            5585..6451
                     /locus_tag="PLQ77_07095"
     CDS             5585..6451
                     /locus_tag="PLQ77_07095"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPL10479.1"
                     /translation="MFGWLTKKRRKKILEAPFPFEWENIIQKRMAHYRMLNEDEQKRL
                     CERIAVFMAEKNWEGCGGLELTDEIRVTISAMACLLILNLPRQGYENVESILVYPSER
                     VLPEPKIGFFETALAPLETPGPISGQAFEQGPVILVWDAVAESIRYAGSGYNVVYHEF
                     AHKLDMQDGVADGTPRLRDRAEYRDWVKTCSREYQKLLKDVERGKRTFLDDYGAKDEV
                     EFFAVSTEHFFDQPLRMRRAAPELYRVLKNYYRQDPAAREGQYAAAREEQTRETGSAF
                     AKAPDAKKGESE"
     gene            6448..6795
                     /locus_tag="PLQ77_07100"
     CDS             6448..6795
                     /locus_tag="PLQ77_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10480.1"
                     /translation="MIDYILRHEAYVRDVLAGHSEKETLAELLAYHDKQISWVQQERL
                     AHLITMMFVCLFFLLAFGWTLIHFTAPYILLTALLLILSAAYILHYYRLENSVQRWYS
                     LSAQIREKLLQSK"
BASE COUNT         1625 a         2006 c         1806 g         1497 t
ORIGIN      
        1 aaatccaggt tggccatggg agaaagctcc cggacgccca ccgcgccgat cgtcgcggcg
       61 gtcgtctccg actccagcga cgcgccgata aacatgccgt gggcccagtc aaagctctgg
      121 tacacgggca cggacgtgtc ggaatcgcgc ccgccgtaaa tgatgccgct cacgggaacg
      181 ccttccggat cgtagagttt ggggtccacg tttttcagtt cgttgaggcg gatcgtgtag
      241 cgggcgttct tgtgggccag cagaatctcg ttgccctttt catccttctt gccttttttc
      301 cactcgccgg aaaaattatg gccctcctcc ggaagaatcc ggcccatgcc cagccagtag
      361 ggtttgtcgt ctttcaccag gacgttggag aagataagct cgcgcggcgt cgtcagcgct
      421 tcgtaaatca ccgggtcgtc gatgggattg acgtcttcga taatgccgaa gatgccctgt
      481 tcgatattga ccgcatacgc ccggccgtcc tcgccgtggc ggatgtaggc gatgtcgtcg
      541 ccgacaatct gctggcccgg caccatggcc gtcgaggttt tgccgcaggc gctgggaaac
      601 gcgccggtaa agtaggtcac gcgcttcctg ccttccggct tcacgcccat gatgaacata
      661 tgttcggtga gccagtcctc tttgttggcc ttgtagatcg ccagacgaag cgccagtttc
      721 ttcaacccga cgctgttgcc cgcatactga ttgttgacgg tcagcacttt tttcccgatc
      781 acgtcgatgt aaatcctgcg gtccttgatg tttttggtgt tgccccgctc atcgagttcg
      841 cccgcggaat ggaccaggta gaaaaaatca tccgacccgt tcagggtctt gaaatgctcg
      901 tagccgaccc gatagagcaa atcttcgctg tgggaaacat aagcggaatc ggtgatctgc
      961 agggcggcga tggaaaaacg ggaatggagc ggtcccaggc agaagaaccg gacaaaacat
     1021 tccttgccct tcatgatgcc gtccatgatt ccgcggattt ccgtgaggcc ttcctcccgg
     1081 tctttcacgt cgatgatctt ggacactttc atatcctgcg tcaccagcag gcaggtgttt
     1141 ttcttgtcgc gcccctggtc gtaaaaactg tcgaaatgaa tcgtgtggcc gggcatggcc
     1201 agcttttttt cttcgcccag atccagggcc atctggcgga tgtagccgat atccgcgaaa
     1261 ctgtccgtga tcacggatac tttcgccggc ttgcataacg ccgcatactc ctcaacgatt
     1321 ctttccacat gggggttgtt gagggcttta atcttccgca gattttcttc gctcaaaata
     1381 gtcttatcga tgttcaattg ctcactccct gaattgattt gttgttcctt ccgttcctga
     1441 acacaacacg gcgcagtgat ttgaggactg aagagggtat cgccggcatc ctgaaaacta
     1501 cggtcagcgg atcgcaaacc catttacgcg gcagactcta aaggacaatc ccataaaaat
     1561 caatcgattt ttacattgcg gacaactccc ggggcgccct ttgccggatc ggaaaaaaat
     1621 cgatcggcag aaaaaaaccg atccggaaaa caagcggcaa gccgacccga cagcccgtgg
     1681 tttgcgcagg cggcccggcc ggcagccgaa taagacactt atcgtttatt gcctttgtcc
     1741 ggacggcctt ttcccggtcc gcccttgggg ccgttgccgg gaccacctcc tcttggcggc
     1801 ccgtcatagc ggtcctggta ctttttctgc tgtccgggag gacgacggtc catctccctc
     1861 acaccccagt ggttttcctt ttcccagtgc ttgttgcgct tccagcttcg ccagtttttc
     1921 tgcgcgtcgc cggagggaat acggcgctga tcccactcac ggcctttcca acggcggtcc
     1981 cgataatcgt ttttccaccc cggcggcacc ccccggtaaa acgagggcac tctgtcgtag
     2041 tagttccagc cactgtcata gtaacgggat cgataccagc gcccctccca cggccgccac
     2101 caccagccgc cgtaaaagaa gatatccgca tcgacgtcgg gaaccgcgta aacataggtt
     2161 tccggaatca cgaccacctc cggcggcgcc ggaaaaatga tcggcggcgg aagggggata
     2221 ttgatcccta ttttgacatc cacgtccgcc atggccgaaa tcggcgtcaa cacgaccagc
     2281 gccaggaaaa tcatcccgaa gaaaaacttt ttcattgtac tgtctccttt ctcaagcgat
     2341 gtcgcccctc cggtcaccga aaagggcggc ttgacccacg gttagaacct atcgttttgc
     2401 aggaacgatt tcaagccaat aatcgtccgg atcgttgatg aagtatatcc ccatcttttt
     2461 gttttcaaaa cagatgcaac ccattttttc atgcagcgcg tgcgccgcgg caaaatcatc
     2521 cacccggaaa gccagatgaa actcattgtc ccccaaactg tatggatccg ttcgctctct
     2581 catccaggtc agctccagtt gatgcgctgt ttggccgtcg ccgagataca ccagaacaaa
     2641 actgccgtcc ggcggttcat aacgccgcgc ttccttcaac ccgagcgcct cacgataaaa
     2701 agccagactt ttttccagat tcagcacgtt gaaattgttg tgggcaaaag taaattgcat
     2761 atcctgatcc tctttatgct gttttcatga aagcaaacga cccgatcccg actacaggca
     2821 atccttcatc atcgattcca ccagctccgg cgtgatatcc ctggtctcac ccagtttgac
     2881 catgccgtgc gctttcaaat tctcaacgac ttccggaatg acatccgcgc cgatgttgta
     2941 cgcgcaaaga cgcgtcggca ccttcatgct ttcaaagaaa tcgcgcgttt tttcaatggc
     3001 ggcgtcgatg cgcgcagcct catccccggc cgcgatcccc cacacgcgtt cggcgtattg
     3061 cagaagcttt tccttcttct cccccgcgcg gatcttcagc agcaccggca gcaccaccgc
     3121 cagggatttg ccgtgatcca ggccgcgcag cgctgtcagt tcatggccga tcatatgcgt
     3181 cgcccagtcc tgcggaaccc cgacactgat cagtccgttc aggccgagcg tggcgcacca
     3241 catcaggttg gcgcgcacgt cataattctc cggttcggcc aaggcccgcg gcccttcctc
     3301 gatcagggtg agcagcaggc cttccgcgaa acgatcctgc agctttccgt tgacgggaaa
     3361 cgtaagatac tgctcggtca catggacaaa tgtatccacc acgccgttgc cgatctgctc
     3421 cggcggaagc gtgaaggtct ttgcggggtc cagcacggaa aattttggaa acagcgacgg
     3481 atgcatgaaa ggaagcttgg cgtttaaggc gcgattcgtt atgaccgctc cgttgttcat
     3541 ctcggatccg gtcgccggaa tcgtcaaaac cgttccgaag ggcatggccc cctttgcctt
     3601 cttgccgtag gttgcgacaa tctcccaggg atcgccttca tagggaacgg cggccgcgat
     3661 aaattttgtg ccgtcgatca cggaaccgcc gcctacggcc agcagaaaat caattttgtt
     3721 ttttcgcgcc atctccacgc accgcatcag cgtctcatag gtcggattgg gctcaatgcc
     3781 ttcgaattct tccatcgctc ggccctgaag cgccgccctg acctcatcat atacgccgtt
     3841 gcgcttgatg ctgccgcctc cgtaggtcat caggatcttt gcatccattg gaatttcttc
     3901 ggcaatatcc ttgatcctgc cctcgccaaa cagaattttc gtcgggttgt aaaacgtaaa
     3961 attgttcatg tgactccctc caggttgaac aagtattgcg gtgaagaaaa tatactggaa
     4021 ttattccctg gtttcaatgg aaaagaaggg ggaacaccct tcagccctga cgcataaaaa
     4081 atacacaccg cttatgaccg atgcctgatt ttgcggtacc ggtagtagcg cagcggaatg
     4141 acgatgacga aaaaaggaat catgtacatg gcggcaacca gcggttcgac gggaccaaaa
     4201 aactgggagg agaagacgat cacgagcacg ttgttgatgt ttcccagcat cacggcgccg
     4261 gccagatgat tatgcagggg ccccttccgg aagaaaaata ttccaacggc gcagtaaatc
     4321 gccgccagga caaacacgac gacggtcgcc atgataatca cggacggctt ctcctgaaaa
     4381 aaaggcgcat agcggtaaaa aacgccgagg ttgatcaggg caaacatcaa cagtgagact
     4441 ggaaactgaa tcttcagaat cggcgcgatg agccggggcg tgaggcagcg gatgatttcg
     4501 accagggcaa tcggaacgaa aatgacggtc gcaagcagcc ggagcatggc ccagaaggaa
     4561 atggccgctt ccttcgcgat cacgatcttg acgagcgccg gcagcgtaaa cggcgcgaga
     4621 gcggaggtaa tgaccacgac cagcagaacc agcgacgagt tgcccttgac catgttcgat
     4681 atcatcggcg aaaccacgcc ggtggaaacg cccgtcaaaa gcagggcggc aagcgcgtaa
     4741 tccggagcga ccagataaaa cacgccgtaa aggaccaccg gcagaagcgc gagcttcagc
     4801 accgtaaaac ccaggatttc ccggatgtgg tccctgaacg ctttccagac atccgccgga
     4861 tcaatcgaca gatagcttag aaaaaaattc accatcagac agtaaacggg aaacgcctgg
     4921 aaacgggagc cgaaatcggg gaaaataacc gcgacagcca tggtcaggac cacgacgacg
     4981 atcagaatca aatcatggat tcgaaacgga gtttgcatgg gtttgggata tcagacagtg
     5041 cccgccttat caacctcttt cacctttgca acttcatgcc cgtcactgat cggacgcctg
     5101 cgccctgcgc aagaaacgcc tgtataaaac atagccgtag atcaccaggt tgaccgcgac
     5161 aaccagtccg ccgagaatgt actgagtaag gggcgtgagt cccgccggat aaatcaccgg
     5221 gaccagatag taaacgacaa agtctcccgt gtagggcgct tcaccgccca gccgtcgcag
     5281 atgattctcc agaggggtca gcggacaaat ccatccggca aattcgatga aagcccccca
     5341 gcaggcggcg ggaagatgca gccaggccat tcgcggccgg cgaagaataa gcaggccgcc
     5401 cgccgcgacg aaaaggataa acaaaaaatg aacaatgatg accgcatcgg ccagcagatt
     5461 gtacataacc ttccctgccc ccgccccact atagcgccgc ccggcgtttg gttcaacgat
     5521 aaaccgctgt ttttcatatc cctgttttta tggtacaaag acaaccgttg aacgggggac
     5581 cgcgatgttt ggctggctga cgaaaaaacg gcggaaaaag atcctggagg cgccttttcc
     5641 cttcgaatgg gaaaacatca ttcaaaagag gatggcgcat taccgaatgc tcaatgaaga
     5701 cgaacaaaag cgtctttgcg aacggatcgc cgtgttcatg gctgaaaaaa actgggaagg
     5761 gtgcggcggc cttgaactca cggatgaaat tcgcgtcacc atttccgcca tggcctgtct
     5821 tctgatcctg aaccttcccc gccagggcta cgaaaatgtt gaatccatcc tggtctatcc
     5881 ttccgaacgg gtcctgcccg aaccaaagat cggttttttt gaaaccgcgc tcgcgccgct
     5941 ggaaaccccg ggccccatca gcgggcaggc tttcgaacag ggtccggtca tcctggtctg
     6001 ggacgcggtt gccgaaagca tccgttatgc cgggagcggc tacaacgtgg tttatcatga
     6061 attcgcccat aaactggata tgcaggacgg cgttgcggac ggcactcccc gtttaaggga
     6121 cagagcggag taccgcgact gggtcaaaac ctgttcgcgt gagtatcaaa aactcctcaa
     6181 agacgtggaa agaggaaaaa gaacgttttt ggacgactat ggcgcaaaag acgaggtgga
     6241 atttttcgcc gtctccacgg aacatttttt cgaccagccg ctgcgcatga ggagggcagc
     6301 gccggagctg taccgggttt tgaaaaatta ctaccgccag gaccccgccg cccgcgaagg
     6361 ccaatatgcg gcggcgcggg aagaacaaac ccgtgaaacc ggatcggcct tcgcaaaagc
     6421 gccggacgca aaaaagggag aatcggaatg atcgactata tcttgcggca tgaagcctac
     6481 gtcagggatg tgctggcagg ccattcggaa aaagaaacgc ttgccgaact gctggcctat
     6541 cacgacaagc agatttcctg ggtgcagcag gaacgcctgg cccatctgat cacgatgatg
     6601 tttgtctgtc ttttctttct tctggccttc ggatggacgc tcattcattt cacggcgccg
     6661 tacatcctgc tcaccgcgct tcttctgatt ctttcggccg cctacattct gcattactac
     6721 cgtctggaaa acagcgttca gcgctggtat tccctgtcag cccaaatccg agagaaactg
     6781 cttcaatcaa aataaacaaa ataaaaagcc ccttgtccgg acggacaagg ggctttactt
     6841 tttaccctat gacctatcgc ctatagccga tagccttttt tacattccca ttccgggata
     6901 accgcctccg ggaggcatac cgggcattcc gccg
//
LOCUS       DAOPAF010000079         6876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9838_length_6876_cov_35.477936, whole genome shotgun sequence.
ACCESSION   DAOPAF010000079 DAOPAF010000000
VERSION     DAOPAF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9838_length_6876_cov_35.477936"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLQ77_07105"
     CDS             complement(<1..718)
                     /locus_tag="PLQ77_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPL10481.1"
                     /translation="MVLRQQKKRIALVHPRGSNWFPGRRDITDIANRMVPQGMLSIAA
                     WLTREGHDVFVYDCLGPGAPVDIDRQARDILAFDPKIVGFSATTSSFPDAAEIAQKIK
                     GAKPGTVTVCGGVHASALREELLADYPAFDYLAAGEGEQTMGELAAGKDPAAIAGLIR
                     RENGAAVVNEPRKPIADLDDLPFPAYEKLRGFPKDYHLPLFSYIRIPGATMITSRGCM
                     YACSYCDRSVFQKGFRYNSAA"
     gene            complement(730..1740)
                     /locus_tag="PLQ77_07110"
     CDS             complement(730..1740)
                     /locus_tag="PLQ77_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPL10482.1"
                     /translation="MHRANEKDKTVNSLPIRAAGYLGPALIPNADGIWEEQKLPANPD
                     ELVQKMMGQPMRRAGRFIKLVCCGALACMSRVSEEALSGRKIGIFLATGLGNIDEILP
                     FITQVFKHDGGFPSPNQFANSVSNSAAFFLAKFCGIKGVILTVSQEELSFESALWLAQ
                     SYLENGEIDLALVGGCDVFTPSVEDYRERLNLIPGRYRSAPMGEASGWLLLGGDGEEK
                     IGDILSVEISSGSANAESLSSDDFLKRCEKLAAGGKGISEDDIINKVLLPGFRLANLD
                     EASWRNSGWDMDDYLVQCGISSTASACGLARALLSRQGAFLVHGNFSSSGRQALIAAS
                     TE"
     gene            complement(1795..2964)
                     /locus_tag="PLQ77_07115"
     CDS             complement(1795..2964)
                     /locus_tag="PLQ77_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPL10483.1"
                     /translation="MKQGNIHIRGWSAVTALGWGASEGARRLIAGDVRPAGVCGSSHL
                     VNREFKAFEVPYDGNPLEKSLKMLDASITEAFERGGLSASEIAESALLVGTTGGIFIR
                     NEYEFTESVRRNPGKESPPIVCRNRGPGEVADVIAGKYGIRGLLMTFSMACVSSSHAL
                     VVAADLLAQKRIKRAVVVGFEPLLNLTLQGFASLLLYDPDECRPFCNRRMGMQIGEAA
                     SCVILEENDSAAAGKSGFVFAGGHLYNDNSSLTSAGTDGEMVRGVVSRTLLKAGTDPG
                     EIIAVKAHGTGTRDNDLSEGRGLAKVFGEGLPPVVSLKGVIGHTLGAAGTVETALWLS
                     CLEEGVIPKSFCFAETDPEIGFAPSQENLPAADGSYLFTFFGFGGTCTGYVVTKR"
     gene            complement(2961..3272)
                     /locus_tag="PLQ77_07120"
     CDS             complement(2961..3272)
                     /locus_tag="PLQ77_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10484.1"
                     /translation="MDLEQAYRDLNVTSREDLLFKLKELVIRACEIRDVRTEDVPTDV
                     PFIGGPGPLKLDSLDAMEIAMELRYTFGVELKNASTAAKAMQSFDTLADFVLAAPKVK
                     K"
     gene            complement(3263..4381)
                     /locus_tag="PLQ77_07125"
     CDS             complement(3263..4381)
                     /locus_tag="PLQ77_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL10485.1"
                     /translation="MCRQFTELVRKEILVLIRDRQTILLLFLMPVALIFFMSLALEGV
                     WADKLIGRKIPTVVENRSGSPQASLLEGKIKAHALIVPVEKPAGSDVDEIFAQGLAQA
                     VVTIPPGFEEGDRPVEIFFDPVMDASYRIAARSLITSLVVEVVMGVDHLEAVVAGLVV
                     ERSGPNRELPSPLQQTVPAYAIFAMFFIAIPMSVGFLREKKDGTLQRLFTYPVSANLV
                     TLGKITPYFLINVVQFVLMMLVGVFVMSRFITPAFFAGQHPWHMLPVTLVVAAATTGF
                     GVLVAATAKTPEQSSTLAATGAILMGVFGGIMVPHAVMPPVMKKLAMISPMFWAHQAY
                     LDIFLRDAAFGIILPRLIILTLFAGICFYIAGRKVRWI"
     gene            complement(4547..5266)
                     /locus_tag="PLQ77_07130"
     CDS             complement(4547..5266)
                     /locus_tag="PLQ77_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL10486.1"
                     /translation="MNALDISSVTFRYDGASAPALRDFSLTVADGQMHALLGPNGAGK
                     STLMRIVTGQLRGHSGEVSVYGLATPDPKALSFIGCAPQPISLYPSLTAWENLRFFGV
                     MAGLSKDQIAQRSEWALEKTGLAEYSESAVASFSGGMQRRLNLAVALLHSPKLLLLDE
                     PSAGVDPQSRNHMYDLLAELNAGDVTILLCTHIMEEAQRLCSGLTLLDRGERIYDGRM
                     SDIDNLEEFFLEKTGRGLRDP"
     gene            complement(5263..6297)
                     /locus_tag="PLQ77_07135"
     CDS             complement(5263..6297)
                     /locus_tag="PLQ77_07135"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPL10487.1"
                     /translation="MTTEVTAAGKPFVHVHSAHCESGVTASLFRDKGVEISEPMIFGI
                     GSGIFFGHLPFVKMAGVTISTFRSQPGAVFRKAAKRLGGKFKTARFRNPKKGMDELRR
                     VLATGQIVGLTTNIYWLSYFPNRFRFQFNGHNVVVLHEIENGFRVSDPMLEQPVDCSA
                     EDLERARFARGPLEPRGFMYYPQSVNSSPDLRAASLKGMKDTCNMMLKIPVPIFGIRG
                     MRYLAKKIVRSPEKLGFMGACRELSSVVRMQEEVGTGGAGFRYMYAAFLQEAAELFAS
                     EELARLSGEMTAIGDIWREFAVVSARIIKQRGQAEETFEKAGGLMLVCAGREEQLFKD
                     LAQAVGKLKA"
     gene            complement(6317..6715)
                     /locus_tag="PLQ77_07140"
     CDS             complement(6317..6715)
                     /locus_tag="PLQ77_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10488.1"
                     /translation="MNVREVPQDITYYEGKKRACYALNDEGKYVIVTSSGWEVEAVVN
                     GLAVDEMAAALEATRQAVLAGAKSPLCYHMERRQMTPEILGKTARLAAFRVKRHLRPD
                     VFAGLSPSVLDRYARALAVSREELKTVPQT"
BASE COUNT         1464 a         2149 c         1926 g         1337 t
ORIGIN      
        1 aggccgccga gttgtagcgg aagcccttct gaaaaacgga gcgatcgcag taggaacagg
       61 catacatgca gccgcgggag gtgatcatcg tcgcgccggg gatccggatg tagctgaaaa
      121 gcggcagatg gtagtctttg ggaaatccgc gcagtttttc gtaggcggga aaaggcaggt
      181 cgtcgaggtc cgcgataggc tttctcggtt cgttgacgac ggcagctccg ttttctctgc
      241 ggatcaggcc cgcgatcgcc gccggatctt tgcctgccgc cagctcgccc atcgtctgct
      301 ctccctctcc cgccgcaagg taatcaaagg cgggatagtc cgccaggagt tcttctctca
      361 gggcggaggc atgcacgccg ccgcataccg tgacggttcc gggcttcgcg cccttgatct
      421 tttgcgcaat ttcggccgcg tcgggaaagg atgacgtggt ggcggaaaag ccgacgatct
      481 tagggtcgaa cgccagaatg tcccgggcct gccggtcgat atccaccggc gcgccggggc
      541 cgagacagtc atagacgaaa acgtcatgcc cttcgcgcgt cagccaggcc gcgatggaga
      601 gcatcccctg cggcaccatc cggttggcga tgtcggtgat gtcgcgtctg cccggaaacc
      661 agttggaccc gcggggatga accagggcaa tgcgtttttt ttgttgacgt aaaaccatga
      721 tcttatttgt tattccgttg acgccgcgat cagggcctgt cttccggacg aagaaaaatt
      781 tccgtgaaca aggaaagcgc cttgacggga aagcagcgcc cgcgccagtc cgcaggcgga
      841 agcggtggag gagatgccgc actggaccag gtaatcatcc atgtcccagc cgctgtttct
      901 ccacgaggct tcgtcgagat tcgccaggcg aaaaccgggc aggagcactt tgttgattat
      961 atcgtcttcg gaaatccctt ttcctccggc agccaatttt tcacatcgct tcagaaaatc
     1021 gtctgaagac agactttctg cgttcgctga tccgctcgag atttcaacag acagaatgtc
     1081 tccgattttt tcttccccat cgccgcccag taaaagccag ccgctggctt cacccatcgg
     1141 agcgctccgg taccggcctg gaatcaaatt cagccgctcc cgatagtctt ccacagacgg
     1201 cgtgaacacg tcgcagcctc cgaccagcgc cagatcgatc tctccgttct ccaggtagct
     1261 ctgcgccagc cacagggcgg attcaaaaga cagctcttcc tgcgaaacgg tgaggataac
     1321 gcccttgatg ccgcaaaact tcgccaggaa gaacgcggcg gagttgctga cggaattggc
     1381 gaactgattg gggctgggaa atccgccgtc atgcttgaag acctgcgtga tgaacggcag
     1441 aatctcgtcg atgttgccca ggccggtagc caggaaaatg ccgattttcc gccccgagag
     1501 cgcttcttcg gaaacacggg acatacaggc cagcgcgccg cagcagacca gcttgatgaa
     1561 ccggcccgcg cgtcgcatgg gctgacccat cattttctgc accagctcgt cgggattggc
     1621 aggcagtttc tgctcctccc agataccgtc cgcgttgggt atcagcgcgg gccccagata
     1681 tccggccgcc cggataggca gggaatttac agttttgtct ttttcattgg ctctatgcat
     1741 atttttcaac atttctgggc ggtccgaatc gtagggaacg gtcgcgaccg ttccctacct
     1801 tttagtaacc acataccccg tgcaggttcc gccgaagccg aagaaggtaa acaaatacga
     1861 tccgtcggct gccgggaggt tttcctgcga cggcgcaaaa ccgatttcgg gatccgtttc
     1921 ggcaaaacag aagctcttcg gaatgacgcc ttcctccagg cacgaaagcc acagagccgt
     1981 ctcgaccgtc ccggccgcgc ccagtgtgtg gccgatgacg cctttgagcg agaccacggg
     2041 cggcagacct tctccgaaaa ccttcgccag ccccctgccc tcggaaaggt cgttgtctct
     2101 cgtacccgtg ccgtgcgcct tgacggcgat aatttcccca gggtccgtgc cggctttcaa
     2161 aagcgtccgg gaaacaacgc ccctgaccat ttcgccgtcc gtgcccgcgg aggtcaggct
     2221 gctgttgtcg ttatacagat ggccgccggc aaacacaaaa ccgcttttgc ccgcggcggc
     2281 gctgtcattt tcttccagga tcacgcagga ggccgcctcg ccgatctgca tgcccatacg
     2341 ccgattgcag aagggacggc actcgtccgg atcgtaaagc agcagactgg caaaaccctg
     2401 gagcgtcagg ttcagaagcg gctcgaagcc gacaacgacc gctcgcttga ttcttttctg
     2461 cgcaagcaga tccgccgcca caaccagcgc gtgcgaactg gacacgcagg ccatgctgaa
     2521 cgtcatcagc aggccgcgaa tgccatattt cccggcaatc acgtcggcca cctcgcccgg
     2581 cccccggttg cggcagacaa tcggcggcga ttctttgccg ggattccggc ggacggattc
     2641 ggtgaattca tactcgttgc ggatgaagat gccgccggtg gtgccgacca gcagggcgga
     2701 ttcggcaatt tcggacgcgg aaaggcctcc gcgctcaaag gcttcggtaa tcgacgcgtc
     2761 cagcatcttc agcgattttt ccaggggatt gccgtcatag ggaacttcaa acgccttgaa
     2821 ctcccggttg accagatggg acgatccgca aacgcccgcc gggcggacat cgcccgcgat
     2881 caggcggcgg gcgccttccg acgcgcccca gcccagcgcc gtgacggcgc tccagccgcg
     2941 aatatggatg ttcccctgtt tcactttttc accttcggcg cggcgagcac aaaatcggcc
     3001 agcgtgtcga aggactgcat ggccttcgcg gccgtggacg cgtttttcag ctccacgccg
     3061 aacgtgtagc gcagctccat cgcgatttcc atcgcatcga gcgagtcgag cttgagcggc
     3121 ccgggtccgc cgataaacgg cacgtcggtg ggcacgtcct ccgtccggac atcccggatt
     3181 tcgcaggccc ggatgaccag ttccttgagc ttgaaaagga gatcctcccg cgacgtcacg
     3241 ttcagatccc tgtacgcctg ttctaaatcc atcgaacctt cctccccgca atataaaagc
     3301 aaattcccgc aaacagggtc aagatgatta acctgggcag aatgatccca aacgcggcat
     3361 cccgcagaaa aatatccaga taagcctgat gcgcccagaa catcggcgaa atcatggcca
     3421 gctttttcat caccggcggc atcactgcgt gcggcaccat gatcccgccg aaaacgccca
     3481 tcaaaatcgc tcccgtcgca gccagcgtgg aagactgctc gggcgttttg gccgtcgccg
     3541 ccaccagtac gccgaacccc gtcgtggcgg ccgcgaccac cagcgtcacc ggtagcatgt
     3601 gccagggatg ctgccctgcg aaaaacgccg gggtaataaa gcgggacatg acaaaaaccc
     3661 ccaccagcat catcagaaca aactgaacaa cgttgatcag gaagtaggga gtaattttgc
     3721 ccagcgtcac cagattcgcg gacaccgggt acgtgaagag acgctggagc gtcccatctt
     3781 tcttttctct caggaaaccg acggacatgg gaatggcgat aaagaacatc gcgaagatgg
     3841 cataggccgg gaccgtctgc tgcaagggac tcggcagctc ccggttgggt ccgctccgct
     3901 cgacgaccag gcccgccacc accgcctcca gatggtcgac gcccatgacc acttccacga
     3961 caagcgacgt gatcagcgag cgggcggcaa tcctgtagct tgcgtccatc acgggatcaa
     4021 agaaaatttc caccggccgg tctccttctt cgaaaccggg gggaatcgtc acgacggcct
     4081 gcgctaagcc ttgcgcaaag atttcatcca catccgaacc ggccggtttt tccaccggca
     4141 cgatcagcgc atgggccttg atttttcctt ccaggaggct ggcctgggga gaaccgctcc
     4201 ggttctccac caccgtgggg attttccgtc cgatcagctt gtccgcccac acgccttcca
     4261 gcgccagcga catgaagaaa atcagggcca ccggcatcag gaaaagaagc aggatggtct
     4321 gccggtcgcg aatcaggacc aggatttcct tgcggaccaa ttctgtaaac tgtcggcaca
     4381 tcaaatgact ttcacccttt tataaaactt ccccaacttc acaaaaacgg attatgcaaa
     4441 gcagaacgag gcggcaagga gcaggccccg caggcgtatt gctaatacgt cgaggacgcc
     4501 tgcgacaccg ccaacaaagt tatgcgcaga agaatccgtt tttgtcctaa ggatcacgca
     4561 gccccctgcc tgtcttctcc agaaaaaact cttccaggtt gtcaatatcg gacatccggc
     4621 cgtcataaat ccgctcgccg cggtccagca gcgtcaggcc agagcacagg cgctgggcct
     4681 cttccatgat gtgcgtgcaa agcaaaatcg tgacgtcccc cgcgttgagc tccgccagca
     4741 aatcgtacat atggttccgg ctctgcggat cgacacccgc tgaaggttca tccaggagca
     4801 ggagcttcgg ggaatgaagg agcgccaccg ccagattaag tcgtctctgc atgccgccgg
     4861 aaaaagacgc cacggccgac tcggaatact ccgcaagacc cgttttttcg agcgcccatt
     4921 ccgagcgctg tgcgatttgg tccttcgaaa gcccggccat cacaccgaaa aagcgcaaat
     4981 tctcccacgc ggtcagagag ggataaagcg agatcggctg cggcgcgcac ccgatgaacg
     5041 aaagcgcttt cgggtccggc gtggccaggc cgtaaacaga cacctctccg gaatggcccc
     5101 ggagctgccc ggtaacaatc cgcatcagcg tggacttccc cgcaccgttg ggccccagga
     5161 gcgcgtgcat ctgtccgtcc gctacagtca gggaaaaatc ccgaagcgcg ggagcggacg
     5221 ctccgtcgta gcggaaggta acggaagaaa tgtcgagcgc gttcatgcct tgagttttcc
     5281 caccgcctga gccaggtcct tgaaaagctg ttcttcgcgc ccggcgcaga ccagcatcag
     5341 cccccccgcc ttttcaaagg tctcttccgc ctgtccgcgc tgcttgatga tgcgcgcgga
     5401 gacaacggca aattcccgcc agatgtcgcc gatggccgtc atttcgccgg ataaacgggc
     5461 aagctcctcc gaagcgaaga gttcggcggc ttcctgaaga aacgccgcgt acatatagcg
     5521 gaaaccggcg ccgcccgtgc ccacttcctc ctgcatccgg accacgctgg agagctcgcg
     5581 acaggccccc atgaagccca gcttctcggg gctgcgaacg atctttttag ccaggtaacg
     5641 cattccccga atgccgaaaa tgggcacggg aattttcagc atcatattgc aggtgtcttt
     5701 catacctttg aggctggccg cgcgcaggtc gggactggag ttgacggact ggggatagta
     5761 catgaatccg cgcggctcca gcggaccgcg cgcaaacctc gccctttcca ggtcttccgc
     5821 ggaacaatcc accggctgct ccagcatcgg atcgctcacg cggaaaccgt tttcaatctc
     5881 atgaagaacc acgacattgt ggccgttgaa ctgaaagcgg aaccggttgg gaaagtagga
     5941 aagccagtag atattggtgg tcagcccgac gatctggccg gttgcgagga cgcgcctcag
     6001 ttcgtccata ccctttttgg gattgcgaaa ccgcgcagtt ttgaatttgc cgcccaggcg
     6061 cttggcggct ttgcggaaga cggctccggg ctgggagcgg aacgtgctga tcgtcacgcc
     6121 ggccatcttc acaaacggca ggtggccgaa aaaaatgccg ctgccgatac cgaaaatcat
     6181 cggttcggag atttccacac ccttgtcgcg gaacagcgac gcggtgacgc cgctttcgca
     6241 gtgcgcggaa tgaacgtgga caaagggttt cccggctgcg gtaacttccg tagtcatgca
     6301 agctgcccct ccagaatcag gtttgcggaa cggttttgag ttcttcgcgg gaaacggcca
     6361 gcgccctggc gtagcggtcc agaaccgacg gactcagccc ggcaaacacg tccggccgca
     6421 aatgcctttt gacccggaaa gccgcgagcc gcgcggtctt tcccagaatc tccggcgtca
     6481 tctggcggcg ctccatgtga tagcaaagcg ggcttttagc gccggccagc accgcctgcc
     6541 tggttgcctc cagcgccgcc gccatttcgt caacagccag gccgttgacc accgcctcca
     6601 cctcccagcc ggaactggtc acaatgacat atttcccctc gtcattgagc gcatagcagg
     6661 cgcgcttttt tccctcgtaa taggtgatgt cctgcggcac ttcccggacg ttcattttca
     6721 aagccccgtt aaagatttca atatcttttt atactaattt gaccccgccg caaagcaaat
     6781 tctgcccggc tgcgcccatc tttcaccgga caattttttc accgagaaac ggctcgaaat
     6841 gatgccattc gaaaggatgc gccgccgcaa agcgcg
//
LOCUS       DAOPAF010000080         6832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_9917_length_6832_cov_47.628597, whole genome shotgun sequence.
ACCESSION   DAOPAF010000080 DAOPAF010000000
VERSION     DAOPAF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9917_length_6832_cov_47.628597"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="PLQ77_07145"
     CDS             <1..1661
                     /locus_tag="PLQ77_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL10489.1"
                     /translation="RTGVFVCHCGINIGGVVNVPEVRDYAATLPTVVYTADNLFTCSQ
                     DTAVKMAEVIKEKDLTRVVVASCSPRTHEGLFQENCEKAGLNRYLFEMANIRDQNSWV
                     HMHEPEKATQKAKDLLRMAVAKAQYLKPLKPGQLSVNHQALIIGGGLAGMTAALSLAD
                     QGFASFLIEKEDRLGGNYNHLYKTLEGLDARAHLTGLLEKIHKNPLITVVTSAHIEKI
                     EGFIGNYKTTVKAKDGEKVFEHGIVLVATGAYENKPKEYLYGENAKVKSQRELETMIY
                     EKDAKLAAVKNVVMIQCVGSRDKERPYCSRYCCGEAIKNALELKTADPSRDITILYRD
                     IRTFAFKEDYYKKAREANIKFISYEENRKPEVVAAGDKVEVKVFDPILNEAVNLPADV
                     VALSIGVVPNPENATIGNMLKVPTNQDGFFLEAHVKLRPVDFATDGVFMCGMSHSPKY
                     SDETVTSANAAVSRACLVLSLDFIEAEGKTAYVNKERCSACGLCEINCPYSAIRVNPA
                     EGCAEVNAVLCKGCGVCTASCRMNAVDLNGFNNEEVLAQIFNIN"
     gene            1706..2146
                     /locus_tag="PLQ77_07150"
     CDS             1706..2146
                     /locus_tag="PLQ77_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1922..1924,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPL10490.1"
                     /translation="MAENTANKEWTPKFVAIVCNWCTYAGADLAGISRIQYPTNVRII
                     RVPCTGRINPFYIVKALQTGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLNYIG
                     VEGDRTIFTWVSASEGDRWAQVIKGATEKIRALGPANKMVKQIG"
     gene            2189..3199
                     /locus_tag="PLQ77_07155"
     CDS             2189..3199
                     /locus_tag="PLQ77_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10491.1"
                     /translation="MENIETKLREEAKRLLLEKKAEVIIGYEKGTLPLTATPCFITKP
                     EEADKLVWNNLCSQNLAKFVHDLITQHKNSQKRVKPEDRKRKIIGVVARGCTTRSMVI
                     HLQERQYERDDVCIIGVPCDGYVDGKGLKVKIDGADIMDGAVEGGQIKMKTSDGDKTV
                     VLKDVLADCCKTCRFNNPIISDVMIGSPAPPMNPDAEYDEVNAFEAKAMEERWAYFTK
                     EMEKCMRCNACRQACPSCYCPVCFVEQSQPQWVGIGEDATDTQVFQFMRLYHMVGRCV
                     DCGSCVAVCPMGVDLRKFLKKLDKDCFEFFGNRAGSGMEDMPPLGKFDEHHDKGDFVY
                     NP"
     gene            3223..3750
                     /locus_tag="PLQ77_07160"
     CDS             3223..3750
                     /locus_tag="PLQ77_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10492.1"
                     /translation="METFLEEVNKKIHGVPLQACYHCRKCSAGCPAASYMEYNPNKVI
                     KMIQNGQKDKLLSSSTIWLCLSCETCITRCPNQVDIARMMDVLRQMAIEEGVAAKEAN
                     VLKFHEAFLSSIKFGGRVHEMLMTVHYKLKSGDLFSDADLAPTMLLKGKLALLPPRTR
                     DLKSVRGIFEKTKSK"
     gene            3822..4670
                     /locus_tag="PLQ77_07165"
     CDS             3822..4670
                     /locus_tag="PLQ77_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPL10493.1"
                     /translation="MKVSYYPGCSLHAMGKEYDQSMKAVSRALNIELKEVDDWSCCGA
                     SAAHNTNFDLSIALPARNLIAAEKDAMDVMVPCAACFNRFKMAEHHLKSDKDLKARIE
                     GVLGARYQGGISIRNPIDVIYNEIGIDALAAKVVKPMTGLKPVSYYGCLLLRPPEVCQ
                     FENYENPFMLDKMMGALGADVKNWSYKTDCCGGSLSVSKTPIVVAMCDKLMAAAREAG
                     ANCVVTACPMCMANLEMRPSPNMKMPVFYFTELISLAMGLPGSKDTFNSHLTDPQPLL
                     NQLRLI"
     gene            4739..5761
                     /locus_tag="PLQ77_07170"
     CDS             4739..5761
                     /locus_tag="PLQ77_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPL10494.1"
                     /translation="MEKRLIKKDALAGIARKMAGDAMVWAPVKKEDNVLFAPLGTGDE
                     PVFTYLNSKNAPKNVFFPHTETLMKFTRTPKGMEFSSEVNQSGQSVLFGVRPCDAHSF
                     VLLDKLFDQEKYKDAYYIEKRKNTTVISLACVKPPYATCFCTSVDGEPVSADGADILL
                     TDLGGDYLVEFITDKGEKLASYFGDAKADAAADAKKAEIAAKARDAIQSKIPAHEIKP
                     ILDVNFDNPFWDTIHRKCLACGTCTFMCPTCHCFDISDEVKGSDGVRIRSWDSCMFPL
                     FTKETSGHNPRSSQKERWRQRTMHKFKYYIDMFNAISCVGCGRCVMACPVNIDIRKVV
                     ADISKL"
     gene            5776..6615
                     /locus_tag="PLQ77_07175"
     CDS             5776..6615
                     /locus_tag="PLQ77_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPL10495.1"
                     /translation="MQNPYIPYPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIP
                     GQFGELSIFGKGESPIGIASSPTQKGYVEFTVQKAGVVTSALHEMEEGTIMGIRGPLG
                     NSWPLDFLEGKNIVIVGGGFAFTTLRSLINYMIYGDNRKRFGNITVIYGARNPGLLLY
                     KDELTEWGKRTDLNLNVTVDKGDATWKGREGFVPAVCKEVAPSKENAVAVICGPPVMI
                     RFTLPVFFELGFSKENIITSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLT
                     PEY"
BASE COUNT         1713 a         1791 c         1913 g         1415 t
ORIGIN      
        1 ttcgcaccgg cgtgtttgtc tgccactgcg gcatcaacat cggcggcgtg gtgaatgttc
       61 ccgaagtccg cgactatgcg gcgacgctgc ccacggtcgt ttacacggcg gacaacctgt
      121 ttacctgctc gcaggacacg gccgtgaaga tggcggaagt catcaaggaa aaggatctga
      181 cccgcgtggt cgtcgcttcc tgctcgcccc ggacgcacga aggcctgttc caggaaaact
      241 gcgaaaaggc cggattgaac cgctacctgt tcgagatggc caacatccgc gaccagaact
      301 catgggtgca catgcacgag ccggaaaaag ccacccagaa agccaaagac ctgctccgca
      361 tggcggtggc gaaggcccag tatctcaagc cgttaaaacc ggggcagttg tccgtcaatc
      421 atcaggcgtt gattatcggc ggcggccttg ccggcatgac cgcggcgctg tcgctggccg
      481 atcaggggtt tgcctccttc ctcattgaaa aggaagatag actgggcggc aactacaacc
      541 acctgtataa gacgctcgaa ggactggacg cgcgcgctca tttgacggga cttctggaaa
      601 aaattcacaa gaatccttta atcactgtcg ttacctcggc ccatattgaa aagattgaag
      661 gatttatcgg caattacaaa accaccgtca aggcgaagga cggcgagaaa gttttcgagc
      721 acggcatcgt tctggtcgcc accggcgctt atgaaaacaa accgaaagag tacctctacg
      781 gagaaaacgc caaagtgaaa tcgcagcgtg agctggagac gatgatctat gagaaagacg
      841 cgaagctggc cgccgtgaag aatgtggtca tgattcaatg cgtcggcagc cgcgacaagg
      901 aaagacctta ctgcagccgt tactgctgcg gcgaggccat caagaatgcg ctggaactca
      961 aaacggccga tccgtcccgc gacattacca tcctttatcg tgatatccgc accttcgcgt
     1021 tcaaggaaga ctattacaag aaagcccgtg aagcgaacat caaattcatc tcctacgaag
     1081 aaaaccgtaa gcccgaagtc gtggcggcgg gcgacaaagt ggaagtgaag gttttcgatc
     1141 cgattctcaa tgaagcggtc aatctgcctg cggatgtcgt agccttaagt attggcgtcg
     1201 tgccgaatcc ggaaaacgca accatcggca atatgttgaa agttcccacc aatcaggacg
     1261 gtttcttcct ggaagcgcac gtcaaattgc gcccggtgga tttcgcaacc gacggcgtgt
     1321 tcatgtgcgg catgtcacat tcgcccaagt atagcgacga gaccgttaca tcggccaacg
     1381 ccgccgtttc gcgcgcctgc ctggtgttga gtcttgattt catcgaggcg gaaggcaaaa
     1441 ccgcttatgt gaacaaggaa agatgttcgg cctgcgggct ttgcgaaatc aactgtccct
     1501 acagcgcgat tcgcgtgaat cccgcggaag ggtgcgccga agtgaacgcg gttctttgca
     1561 aaggctgcgg tgtctgcacg gcgtcctgcc gcatgaacgc ggtggacctc aacggcttca
     1621 acaacgaaga agtgctggcg cagatattta atatcaatta aggcgaacgg ctgatcaggc
     1681 cgcattgccg ctggaaggag aatatatggc tgaaaacaca gcaaacaagg aatggacacc
     1741 aaaattcgtt gccattgtct gcaactggtg cacttacgcg ggagccgatc tggcggggat
     1801 cagcagaatt cagtacccga ccaacgtgag aattatccgc gttccctgca ccggaaggat
     1861 caatcctttc tacatcgtga aggcattgca gaccggcgcc gacggcgttc tggtttcggg
     1921 ttgacacccg ggtgaatgcc actatatctc aggaaatctg gtggcgcggc gcaagtttgc
     1981 gacgttaaaa agtttcctga actacatcgg tgtggaaggc gaccggacca tttttacctg
     2041 ggtctccgcc tccgaaggcg acagatgggc gcaggtcatc aaaggcgcaa cggagaaaat
     2101 ccgggcgctg ggaccggcca ataagatggt caagcagatt ggttagtctg attcaggatc
     2161 ataatacata aacggggaaa tcatcactgt ggaaaacatc gaaacaaaat tgcgggaaga
     2221 agcaaagagg ctgcttttgg aaaagaaggc cgaggtgatt atcggctatg aaaaaggaac
     2281 gcttccgttg acggcgacgc cttgtttcat cacgaagccg gaagaggccg acaaactggt
     2341 ctggaacaac ctctgctcac agaacctggc caagtttgtg cacgatctga tcacgcagca
     2401 caaaaattca cagaagcgcg tgaaaccgga agaccggaag aggaaaatca tcggtgtggt
     2461 tgcccgcggc tgcacgacgc gctccatggt catccatctg caggaaagac agtatgaacg
     2521 cgacgacgtc tgcatcatcg gcgttccctg cgacggctac gtggacggca aaggactgaa
     2581 agtgaaaatt gacggcgcgg atatcatgga cggcgccgtc gagggcggcc agatcaagat
     2641 gaaaacgtcc gacggcgaca agaccgtcgt gctcaaagac gtgctggcgg actgctgcaa
     2701 gacctgccgc ttcaacaatc cgattatttc cgacgtgatg atcggatcgc ccgcgccgcc
     2761 catgaacccg gatgcggaat acgacgaagt caacgcgttc gaagccaagg ccatggaaga
     2821 acgctgggcg tatttcacca aggaaatgga aaagtgcatg cgttgcaacg cctgccgcca
     2881 ggcctgtccc tcctgctact gtccggtttg ctttgtggag cagagccagc cgcagtgggt
     2941 tggcattggc gaggacgcga ccgatacgca ggtgttccag ttcatgcgtc tgtatcacat
     3001 ggtcggacgc tgcgtggact gcggttcctg cgtcgccgtc tgtccgatgg gcgtggatct
     3061 gcgcaaattc ctcaagaaac tcgacaagga ttgctttgaa tttttcggca accgggccgg
     3121 gtccggcatg gaagacatgc cgcccctggg gaaattcgat gaacatcacg ataaaggcga
     3181 ttttgtctac aacccgtaat tgaatgagat gatggagttt acatggaaac ttttctggaa
     3241 gaagtaaaca aaaaaataca cggcgttccg ctgcaggcct gctatcactg ccggaaatgc
     3301 tcggccggat gtcccgcggc gtcctacatg gaatataacc ccaacaaggt gatcaagatg
     3361 atccagaacg gccagaagga caagctgctg tccagttcga cgatctggct ttgcctgtcg
     3421 tgtgagacct gcattacccg ctgccccaac caggtggaca ttgcgcggat gatggacgtt
     3481 ctccgccaga tggcgattga agaaggggtt gccgcaaaag aggccaacgt tctcaaattc
     3541 cacgaggcgt tcctgtccag catcaagttc ggcggacgcg tccatgaaat gctgatgacg
     3601 gttcattaca aacttaaatc cggagacctc ttttcggatg cggaccttgc cccgaccatg
     3661 ctcctgaagg ggaaactggc gcttctgccg cccaggacgc gggatctgaa atcggttcga
     3721 ggcatttttg agaaaacaaa gagcaaatga tggaatatcg cgcgaaaggc ccccggcggc
     3781 cagggcgcgc tgaagtaaca gtttaaaagg aggctactct cttgaaagtt tcatattatc
     3841 cgggttgctc actgcacgcg atgggaaaag aatacgatca gtccatgaag gccgtcagcc
     3901 gagccctcaa catcgaactc aaagaagtcg acgattggtc ctgctgcgga gcgtcggccg
     3961 cgcacaacac gaatttcgat ctgtccatcg ccctgccggc gcgcaatctg attgccgcgg
     4021 aaaaggacgc aatggatgtc atggttccct gcgccgcctg tttcaaccgc ttcaaaatgg
     4081 cggagcatca cctgaagtcc gacaaggacc tcaaagcccg catcgaagga gtgctgggcg
     4141 ccaggtatca gggcggcatc tccatccgca acccgattga cgtgatttac aatgaaatcg
     4201 gcatcgacgc actggccgcg aaagtcgtaa aacccatgac cggcctcaag cccgtgtcct
     4261 attacggctg tctgctgctt cgtccgccgg aagtctgcca gtttgaaaat tacgaaaacc
     4321 ccttcatgct cgacaaaatg atgggagcgc tgggggcgga cgtgaagaac tggtcgtata
     4381 aaacggactg ctgcggcggt tcgctctcgg tcagcaaaac ccccatcgtg gtggcgatgt
     4441 gcgacaagct gatggctgcg gcgagagaag cgggcgccaa ctgtgtggtc acggcatgtc
     4501 cgatgtgcat ggccaacctg gaaatgcgcc ccagtccgaa catgaagatg ccggtgttct
     4561 attttacgga attgatttcg ctggcgatgg ggctcccggg ttcgaaggat accttcaatt
     4621 cgcacctgac cgatcctcag ccgcttctga atcaactgcg tttgatttga tcggtgagga
     4681 aacaacaccc gacgctttga aactgataag ctggataaaa aattaattag ggataattat
     4741 ggaaaaacgt ttgatcaaga aagatgcgct ggcgggtatt gccaggaaaa tggccggaga
     4801 tgcgatggtt tgggcgccgg taaagaaaga agacaacgtt ctttttgcac ccctcggaac
     4861 gggcgacgag ccggttttca cctatctgaa ttccaaaaac gcgccgaaaa atgttttctt
     4921 ccctcacacg gagaccctga tgaagttcac ccgcacgccg aaaggcatgg aattttcgtc
     4981 ggaggtcaat caatccgggc agtcggtttt gttcggcgtg cgcccctgcg acgcccacag
     5041 ctttgttctg ctggacaagc ttttcgatca ggaaaaatac aaggacgcgt actatattga
     5101 aaaacggaaa aacacgacgg tgatctcgct ggcctgcgtg aagccgcctt acgcaacctg
     5161 tttctgcacg tcggtggacg gagagccggt ttcagccgac ggcgcggaca tactgctcac
     5221 cgacctcgga ggcgattatc tggtggaatt catcaccgat aagggcgaga agctcgcctc
     5281 ctatttcggc gacgccaaag ccgacgcggc cgccgatgcc aagaaagcgg agattgccgc
     5341 caaagccagg gatgcgatcc agtccaaaat tccggcgcat gaaatcaaac cgattctcga
     5401 cgtcaatttc gacaatcctt tctgggacac cattcatcgc aagtgcctgg cctgcggaac
     5461 ctgcacgttt atgtgcccca cctgccactg cttcgatatc agcgacgagg tcaagggatc
     5521 ggacggcgtg cgcatccggt cttgggattc ctgcatgttt ccgctcttca caaaggaaac
     5581 gtccggacac aatccgcgtt cttcgcagaa ggagagatgg cgccagcgga ccatgcacaa
     5641 gttcaagtat tacattgaca tgttcaatgc gatttcctgc gtgggctgcg gccggtgcgt
     5701 catggcttgt cccgtaaaca ttgatatccg taaagtggtt gcagatattt ctaaattata
     5761 atcagggagt tcgtaatgca aaacccgtat attccttatc ccgttgtagt ggataaaatc
     5821 attaccgagg tcgataccaa ggacattaaa acgttccgct tcaaattcgt gaatccggaa
     5881 gacgaacaga aatacgccta cattcccggc cagttcggcg aactctccat cttcggcaaa
     5941 ggcgaatcgc ccatcggcat cgcttcttct ccgacgcaaa aaggctatgt cgaattcacc
     6001 gttcagaagg ccggcgtcgt gacttcggcg ctccatgaaa tggaggaggg aacgatcatg
     6061 ggcatccgcg ggccgctggg caattcctgg ccgctggatt ttctggaagg caagaatatc
     6121 gtgattgtcg gcggcggttt cgcgttcacg acgcttcgtt cgttgatcaa ctacatgatt
     6181 tacggcgaca accgcaaacg gttcggcaac atcaccgtga tttacggcgc gaggaatccc
     6241 ggcctcctgc tgtacaagga tgaattgacg gaatggggca aaagaaccga cctgaacctg
     6301 aacgtcacag tggacaaggg cgatgcgacc tggaagggca gagagggatt tgttcccgcc
     6361 gtctgcaagg aagtggcgcc cagcaaggaa aacgccgtgg cggtgatctg cggccccccc
     6421 gtgatgattc gcttcaccct gccggtcttc tttgagctcg gcttttcgaa ggaaaatatc
     6481 atcacgtcac tggaaatgcg gatgaaatgc ggaatcggca aatgcgggcg gtgcaacgtc
     6541 ggcagcaaat atgtctgcaa ggacgggccg gttttctcgc tcgcggaact ggataaactg
     6601 acgccggaat actagccaac cccgaattct tcaaaactgt tttaaaaggg agcctttggg
     6661 ttcccttttt tttgaagcgg cgtccccgcc tgcgagggcg gggagagcaa aaaaaattga
     6721 tgtttgtttt tttctatgct agcagaagca taaattgatc cattcatgac gggagatgtt
     6781 gcatgtcaca aacggttacc ccctatctgg agatgtccca ggcgatcatg ga
//
LOCUS       DAOPAF010000081         6567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_10373_length_6567_cov_40.593673, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000081 DAOPAF010000000
VERSION     DAOPAF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10373_length_6567_cov_40.593673"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1289)
                     /locus_tag="PLQ77_07180"
     CDS             complement(201..1289)
                     /locus_tag="PLQ77_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPL10496.1"
                     /translation="MEKQTQTEAESTSGVFADCQLGAGFDEEFFLLNFGTRLLTAFTD
                     REILIDIALETLADFSQGKRIAIMSLDEQNKTLEIDGVFFQRQSSRPQMSFPVEKTVA
                     GKWMSQKTVGVAPLILEGEVPLPAESGRPEDSQCLCLPLIGSSFRPVGLATIEIPAGR
                     CFDFFEAQQLRVLSTALAVSLENTKLFSRVIHDSLTNLYTRRFYEIRVEEEIAKLRRN
                     SGRLSIILFDLDQFKNINDSFGHLTGDEVLRQFAALMLDHVRKGSTLISRYGGEEFIL
                     LMPDARVEEALRLAERIVSLCASHEFGDGRRKIQMTVSGGVAFTDHTEAVSQTELFER
                     ADQALYEAKHSGRNRVVVWKNISAPYRM"
     gene            1711..3147
                     /locus_tag="PLQ77_07185"
     CDS             1711..3147
                     /locus_tag="PLQ77_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPL10497.1"
                     /translation="MKTKVIFKKLLSSHQNVSFKNYLPLNSALVSDIGKAVTAIFDEL
                     GGASLLKSSGEVFIKPNAVDAKAYSHTRIEVLREIILYWKKAGAKKIYLFENSTQANY
                     TRLVYSATGYARLCRETGAKPIYLDEEKSRPLPFTGRGPVGVKDPDGYDLTSFEMPET
                     VMRLIEERDRHLYINVPKLKTHSMGVVTLGVKNQWGFPMQKCRGFDHNYNLPQKLVDV
                     LGYIRPDVTLIEGVEGTIHGHYFATALADKQVRPFRVLIAGKNVVAADIAGARVFGLN
                     VDDVPHLRIAIERGFSDGVKGPQDIELSGDIASLEKIDLIGDMPTDGHYPWDLYPEFP
                     PDVTVMKGNKLVCREGCLNNPLCVMQTMYMDNAGKGGWTLVLGRGHDPSVVDGIKGRV
                     LIAGHCAIDEVSEKLIKRLGRNKVYLSGECNNLCATVEAMFHLMKVDPLSYVPLNPLV
                     SSAAFLMARLNGSTSRVPHPLSHIIKRV"
     gene            complement(3272..4234)
                     /locus_tag="PLQ77_07190"
     CDS             complement(3272..4234)
                     /locus_tag="PLQ77_07190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPL10498.1"
                     /translation="MVRDSGAPTDLESLLQGNPEKVTRYLLSSGRMAMILLDRKGVIL
                     DCNAFFLESVGLSEKPVGKSIQAFLREGLQAGWECIRGDCRPVKLTFTLNGPLEYFLS
                     GHILNVQEHYLLFAHTVRLTDHEMVVKLSRLTDELTDLTRQLNKKNRELEAANETITR
                     LMNTDPLTGLTNRRQLKERMDAELSKARRHGHPLSALMTDIDHFKSINDTYGHAAGDA
                     VLVGVASILRQTCRKEDIVARYGGEEFVVLLPDSPAAAALDCAERIRKAIEGNVFPEI
                     DRKVTVSLGATLFAPTDTEDSFIGRADAALYEAKTSGRNRVVLK"
     gene            complement(4228..5325)
                     /locus_tag="PLQ77_07195"
     CDS             complement(4228..5325)
                     /locus_tag="PLQ77_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPL10499.1"
                     /translation="MMEWERLIREARECLQVSPEATARYIKAKDILLEEVNARMTAHP
                     GISNLTGGNPMSMMRDNHSNHLDFMSTVFEFNSFELLVKTVPWVYRSYHSHGFSFDYF
                     PLELEAWKKAVSQFLSPEAAGEINAVYDWMLKNHERMIELSAFIPDQRETYPELKDER
                     RRFGGGLLAADFPLCMKIADSVLERENGQEALYLGLIQPVMYEIGRLWEQDKISAAEE
                     HMATSMVGRILAGLYARFPVPPANRGKAVVTSAPNEFHELGARILADMLEADGWDIFF
                     LGANTPAEELIKLVKKADPRFLAISATMPFNMDRVAAMISGIRNDPALNSVKILVGGA
                     AFNTDHTLWRRIGADAWAEDPPSAIRQVQSW"
     gene            complement(5375..5794)
                     /locus_tag="PLQ77_07200"
     CDS             complement(5375..5794)
                     /locus_tag="PLQ77_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPL10500.1"
                     /translation="MKYLTAIEARQFAEKWLPAWTGNHPELLAGFYSEDAFYMDPAVP
                     RGVRGKNNLLAYFRKLLAVNPNWVWTQIEGIPLEDGFLNKWLAKIPVGSVTLEVVGVC
                     FVQLDDAGKIRRNEVYFDRSALLSEMAKLKRSAPAGP"
     gene            complement(5844..6029)
                     /locus_tag="PLQ77_07205"
     CDS             complement(5844..6029)
                     /locus_tag="PLQ77_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPL10501.1"
                     /translation="MFNFRSPSFKKLSLDPESLQDKDMVERMLQEPRLIRRPVVKIGR
                     KVYFGASADVLADILKS"
     gene            complement(6212..>6567)
                     /locus_tag="PLQ77_07210"
     CDS             complement(6212..>6567)
                     /locus_tag="PLQ77_07210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPL10502.1"
                     /translation="GEAFVKKQLAMDPGACRLGEFSLTDRRFSRIDRFMAETLFDENF
                     GGKEGNCHVALGASYSDTYSGDPARLTPALKKKLGFNDSALHWDLVNTEPKTVSAHLK
                     NGKKIMIYDRGIFTI"
BASE COUNT         1449 a         1692 c         1830 g         1596 t
ORIGIN      
        1 ccgtccagac cgcgccgcat ttcttcatgg aagcggacag cggcgcgttg cgcgtcggag
       61 ggagaaaagg aaaagagatc ctccagattc gatagatatg tgcgaatggt ctgattcata
      121 ctcgccacgc gaaaagaacc gcttttttcc gtcacaggcg ggctttcggc atcggcggcg
      181 ccgttccgaa ttttaaaaga ctacatccgg tagggggcgc ttatgttttt ccagacaaca
      241 acacggtttc gtccgctgtg tttggcttcg taaagcgcct gatccgccct ttcgaaaagt
      301 tctgtctggg agacggcttc cgtatggtcg gtaaacgcca caccgccgct gactgtcatt
      361 tggatttttc tccggccgtc gccaaactca tggctcgcgc acagagagac gattctttcg
      421 gccagacgga gcgcttcctc cacgcgggca tcgggcatga gaaggatgaa ctcttctcct
      481 ccgtagcggc tgatcagcgt ggagcctttg cgcacgtggt ccagcatgag cgcggcgaat
      541 tggcgcagaa cttcgtcgcc cgtcaggtgg ccgaaagaat cattgatgtt tttgaactgg
      601 tccagatcaa aaagaatgat ggacaggcgt ccgctgtttc gtcttagctt ggcaatctct
      661 tcttccaccc tgatttcata gaacctgcgg gtgtaaaggt tggtcagact gtcgtgaatg
      721 acccgggaga aaagctttgt gttttccaga gagacggcca gagccgtgct gaggactcgt
      781 aattgctgcg cctcgaaaaa gtcgaagcag cgtcccgccg ggatttcaat cgtggccagt
      841 cccaccgggc ggaaagagga cccgatcagc ggaaggcaca aacactggct gtcctccggg
      901 cgtccgctct ccgccgggag gggaacctct ccttccagga tgagcggagc gacgccgacc
      961 gttttttgag acatccattt tcccgcaacc gttttttcca cgggaaaaga catttgcgga
     1021 cgggaagatt ggcgctgaaa aaagacaccg tcgatttcca atgttttgtt ctgttcgtcg
     1081 aggctcatga tggcgattct ttttccctgg gagaaatcgg ccagggtttc gagggcaatg
     1141 tcgatcagaa tctccctgtc ggtaaaggcg gtgaggagcc gggtgccgaa attgagaaga
     1201 aaaaattcct cgtcaaaacc ggcgcccagt tgacagtcgg caaaaacgcc ggaggtgctt
     1261 tctgcttcgg tctgagtctg tttttccata tggttccttt tgtacgcgac tataaggtgt
     1321 tgttcaggaa aatgaaacga caaaaggtga aacagcgctc ctttctcctg cggcacggag
     1381 tataggtcag gccagtccgg tctgtcaaag agaatctttt tttggtccat ccggttgatg
     1441 cgcatagcag gtagccgtac ggaagacccg gatggacaaa aatattcata aaaaatcgtt
     1501 tttgaagttg ataacatagg ttgcttttta aaatcggttt gttccaaata cgatcatata
     1561 accaaacttg ccgcagcaag aaatatgctt gacaagcaaa agccgccctc atatgtattc
     1621 actgccatga aaagcgacag gtgcagcaac attcacgcag atatttactc tttacaatac
     1681 gctttgtgaa tcaaatcaaa aagagaagaa atgaaaacaa aggttatctt taaaaagctt
     1741 ctctccagcc atcagaatgt aagttttaaa aattacctgc ccctgaacag cgcccttgtg
     1801 agtgatatcg gcaaggctgt cacagccatc tttgatgaat tgggcggggc gtcgttgctg
     1861 aaatcgagcg gtgaagtttt tataaaaccc aatgcggttg atgcaaaagc ttacagccac
     1921 accagaattg aggttcttcg ggaaatcatt ttgtattgga agaaagccgg cgcgaagaaa
     1981 atatatcttt tcgaaaactc gacgcaggca aactatacaa ggttggtgta tagcgccacg
     2041 ggatatgcaa ggctttgccg tgaaacgggc gcaaagccga tatatcttga tgaagagaaa
     2101 agccggcctc tgccctttac cggtcgcggg cctgtcggag tcaaagaccc cgatggctac
     2161 gatttgacgt cttttgaaat gcccgagacg gttatgcggc tgatagaaga gcgggaccgt
     2221 catttgtata tcaatgtgcc gaaactgaaa acccattcca tgggagttgt gacgcttggc
     2281 gttaaaaacc agtggggctt cccgatgcag aaatgccggg gttttgacca taactataat
     2341 ttgccgcaaa agcttgtcga tgtgctcggg tacatcaggc ctgatgtaac gctgattgaa
     2401 ggcgttgaag gaacgattca cggtcattat tttgccacgg cgcttgccga taaacaggtg
     2461 cggcctttcc gtgtgctgat tgccggtaaa aatgtggttg ccgccgatat tgccggcgcc
     2521 cgtgtttttg gattgaatgt tgacgatgtg ccccatctca gaattgcgat tgaacgcggc
     2581 ttctcagacg gcgtgaaggg gccgcaggat attgaacttt cgggcgatat cgccagcctt
     2641 gaaaaaattg accttatcgg cgatatgccg acggatgggc actacccctg ggacctctat
     2701 ccggaatttc cgcctgatgt gacagtaatg aaagggaaca aacttgtgtg ccgggaaggt
     2761 tgcctgaata acccgctctg cgtaatgcaa acgatgtata tggacaatgc cggcaagggc
     2821 ggctggacgt tggttttggg aagaggtcac gatccatccg tggtggatgg gataaagggc
     2881 agagttctta ttgccgggca ttgcgcgatc gatgaagtat ccgaaaagct catcaaacgg
     2941 ctggggcgga ataaggtata tttaagcggc gaatgcaata atctatgcgc caccgttgaa
     3001 gccatgtttc atctgatgaa ggtagatcct ctttcgtacg tgcctttgaa tccgcttgtt
     3061 tcttcagcgg cattcctgat ggctcgcctg aatggttcga cctcacgggt gccgcacccg
     3121 ctttcgcata tcatcaaacg agtttgaaat gagttggcgt tggcattaga ccttgaaccg
     3181 gacggcaata acgtcgccgt tccgggccgc atcgcctggt cccgtggaag agggaatgag
     3241 cgtctccgcc gttcagcttc gctttggaag gctatttcag caccacccgg tttcgtccgg
     3301 atgtcttggc ctcgtacagg gcggcgtccg cccgcccgat gaagctgtcc tccgtgtcgg
     3361 tgggcgcaaa gagagtggcg cccaaactga cggttacctt ccggtcgatt tccggaaaca
     3421 cgtttccctc aatggctttt cggattcgct ccgcgcaatc cagggctgcc gcggcgggcg
     3481 aatcgggaag gaggacgaca aattcctctc cgccgtagcg ggcgacgatg tcttctttcc
     3541 ggcacgtctg ccgcagaatg gaggctaccc cgaccagcac tgcgtcgccc gcggcgtgtc
     3601 cgtaggtgtc gttgatggat ttgaaatggt cgatgtccgt catgagcgcg gaaagcgggt
     3661 ggccgtggcg tctggctttg gacagttccg cgtccatcct ttccttgagc tgacgccggt
     3721 tggtgagccc ggttaaggga tcggtgttca tcagccgggt gatggtttcg ttggcggctt
     3781 ccagctcccg gttttttttg ttgagttgcc gggtcagatc agtcagttca tccgtgagcc
     3841 gggacagttt gacgaccatt tcatgatcgg tcaggcggac ggtgtgcgcg aaaaggagat
     3901 aatgctcctg gacattcagg atgtggcccg acagaaaata ttccagcggt ccgttcaatg
     3961 taaacgtcag tttcacgggc cggcaatctc cgcggatgca ttcccatccg gcttggaggc
     4021 cttcccgaag aaaagcctgg atggatttgc cgacgggttt ttccgataat ccgacgcttt
     4081 ccaggaagaa ggcgttgcaa tccagaatga cgcccttccg gtccagaagg atcatggcca
     4141 tcctgccgga agacaggaga taccgggtga ccttctcggg atttccctgc agcagacttt
     4201 ccagatcggt tggcgcgccg ctgtctctta ccatgactga acctgccgga tggcggacgg
     4261 gggatcttcc gcccaggcgt cggcgccgat tcgccgccat agcgtgtggt ccgtgttgaa
     4321 agcggccccg ccgaccagaa tcttgacgga gttcagggcg ggatcgtttc ggatgcctga
     4381 aatcatggcc gcgacccggt ccatgttgaa cggcatggtt gccgagatcg ccaggaaacg
     4441 cggatcggct tttttgacca gtttgatgag ctcttcggca ggcgtgttcg cgcccaggaa
     4501 gaaaatgtcc catccgtccg cttccagcat atcggccaga attcttgcgc cgagttcgtg
     4561 aaactcgttg ggtgcggagg tgaccacggc cttgccccgg tttgccggcg gaaccggaaa
     4621 cctggcatag agaccggcca ggattcttcc caccatggac gtggccatgt gctcttcggc
     4681 ggcgctgatc ttatcctgtt cccacagtct gccgatttca tacatgaccg gctggatcag
     4741 cccaaggtaa agggcttcct gcccgttttc ccgctccaga acggagtcgg caattttcat
     4801 gcaaagcgga aagtccgccg ccagaagacc gccgccgaat cttctgcgct catccttcaa
     4861 ttccggatag gtctcccgct ggtcggggat gaacgcggaa agttcaatca tccgttcatg
     4921 gtttttcagc atccagtcgt aaacggcgtt gatttccccg gctgcttcgg ggctgagaaa
     4981 ttggctgact gcctttttcc aggcttccag ctccagtgga aagtagtcaa acgaaaagcc
     5041 gtgcgagtgg taggagcggt atacccaggg gacggttttg accagcagct caaagctgtt
     5101 gaattcaaag acggtggaca tgaaatcgag gtgattgctg tgattgtccc gcatcatgct
     5161 catgggattg ccgccggtca ggttggaaat cccgggatgg gcggtcattc gggcgttgac
     5221 ctcctcaagc aggatgtcct ttgccttgat atagcgagcg gttgcttcgg gagatacttg
     5281 caagcattcc cgtgcttccc ggatcaaacg ttcccattcc atcatttttc tccatgatca
     5341 ggatgttgtt tgcggttcaa tgcacggatt cggctcaagg accggccggc gcgcttcttt
     5401 ttagttttgc catttcggaa agaagagccg agcggtcgaa gtaaacttcg ttgcggcgga
     5461 ttttccccgc atcgtccagt tggacaaagc aaacaccgac cacttccagg gtgacggagc
     5521 caaccggtat tttggccagc cacttgttca gaaatccgtc ttccagcgga atgccttcga
     5581 tttgcgtcca gacccagttc ggattgacgg ccagcagctt ccggaagtaa gccagcagat
     5641 tattttttcc cctgacgccc cggggaacgg ccggatccat gtaaaaagcg tcttccgagt
     5701 agaagccagc cagcaattcg ggatggtttc ccgtccacgc cggcagccat ttttccgcaa
     5761 attgcctcgc ttcaatcgcc gtgagatatt tcatcggatc tttcctttac aatggatttt
     5821 ccttgctctt ttcgtaaacc gtttcaggat ttcaggatgt ccgccaaaac atcggccgaa
     5881 gcgccgaagt aaacttttcg gccgattttc acgaccggac ggcgaatgag ccgcggctcc
     5941 tgaagcatcc gttcaaccat atccttgtcc tgaaggcttt ccgggtccag agacagtttt
     6001 ttgaaactgg ggcttcggaa attaaacatt tccgaaggcc ggcttccgcg cagcagcgct
     6061 ctgatctcgt cggcggtaaa gggttttttg aaaaaatcac gctcgtttac atcgaccttc
     6121 ttttgcgaga gaaactccct cgctttccgg caggtggttc acttttgcca gcaatagaaa
     6181 tcaactttca tatgaagaca gcctccccgg tttaaattgt aaaaatgccc cggtcgtaaa
     6241 tcatgatttt cttgccgttt ttgagatgcg ccgagacggt tttgggctcc gtgttgacca
     6301 gatcccagtg cagggcggaa tcgttgaatc ccagtttttt cttgagcgcc ggggtcagac
     6361 gcgccgggtc gccgctgtac gtgtcgctgt aggaggcccc cagtgcgaca tggcagttgc
     6421 cttccttccc gccgaagttt tcgtcaaaaa gcgtttccgc cataaagcgg tcgattcggg
     6481 aaaagcgccg gtcggtaaga gagaactctc caagccggca ggctccggga tccatggcga
     6541 gctgtttttt cacaaaggcc tcgccct
//
LOCUS       DAOPAF010000082         6498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_10503_length_6498_cov_66.299395, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000082 DAOPAF010000000
VERSION     DAOPAF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10503_length_6498_cov_66.299395"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLQ77_07215"
     CDS             <1..293
                     /locus_tag="PLQ77_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPL10503.1"
                     /translation="GRTSVMSGNTAGFILETVRRKNIEAKGGAVNEEDMETFRQMMRE
                     RYNSEGHPFYTGSLMFHDGVIAFSEARDKLARAFEIALRTPVRESDWGDLKF"
     gene            319..1116
                     /locus_tag="PLQ77_07220"
     CDS             319..1116
                     /locus_tag="PLQ77_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPL10504.1"
                     /translation="MADVLLREQKGEGITLLTLNRPEAMNCFNFDLLGALGDAVRESN
                     FDSGLRCLIITGAPADNPKKASFSTGADLIERRTMPPEQVRRFIFTIRNLFTSVEQLR
                     VPVIAAVNGFAFGGGTELALACDLRIASSNVVMGLTETSLAIIPGAGGTQRLPRIVGL
                     AKAKELIYTARRINAQTALDIGLVNRVVEPEKLIDAACELAREIAANGPIAVAQAKFA
                     INCGMDAPLSVALPLESKAYEITIPTKDRLEALAAFAEKRKPDFKGE"
     gene            1149..2336
                     /locus_tag="PLQ77_07225"
     CDS             1149..2336
                     /locus_tag="PLQ77_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10505.1"
                     /translation="MTEYDYWKIFPRMPKKATIGDITIRDGFQHCEKFISTQAKIFYG
                     QEMILAGCRELEVTNLGNPRAMPQFKDADEVMKAMRGDDFRKRCGKAGIDVDKDVTLT
                     CVTIREAAVDRAIEMRKNGYGPDRILMMVSTDPEHHFANSGTTLSQYWREAERCIQKA
                     RDAGIKMCGTVSTIWGSPISGATKLEDAVEFTRRWFQIGAHDIEHADHDGSANAADAY
                     RYFSMVLDAMPNPEAHLCHLHETKRIASSTILAALQAGICRFEATLGGLGGQPANFLD
                     DCPIKGTGEYYYDPRYVGLICMEDLVVQIDELGIRHGYDVNRVLMLGRKMEKTIGARL
                     RSEAIINGRTVKEGHMEHARPGLKKLIEKEKEKPGQQFPDEWSTEAILPEKWGPADPI
                     WKK"
     gene            2429..3346
                     /locus_tag="PLQ77_07230"
     CDS             2429..3346
                     /locus_tag="PLQ77_07230"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPL10506.1"
                     /translation="MMKKIKIIEVGPRDGLQNLETFVPTDKKIALIKKLAGAGLKEIQ
                     IGSFVSPQSIAQFRDIEEVAKAVRRLRSVVPSALVPNRRGAEKALACGISRLIFFFSV
                     SSKHNYANVKQSPQDSLKELKNICRTYTDLASTSIRVDLATAFGCPFAGNVTTAAVLR
                     MVEKVAALGVQEITLCDTVGFGHPKAVEEKFRKCFRLFPEIRFGAHFHNTRGLAQANA
                     LRAWDIGIRSFDSSVGGLGGCPYAPGSSGNVATEDLVYLFESMGIKTGMDLKKLLNIC
                     AWLKQILPQAKLSGSLFQAGLPRTHKLSC"
     gene            complement(3447..4304)
                     /locus_tag="PLQ77_07235"
     CDS             complement(3447..4304)
                     /locus_tag="PLQ77_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HPL10507.1"
                     /translation="MGSTLRKSLKTIEEYKTANPHYRDLLDILGDILILREEYRKNMT
                     KPIFSVEDKLIPGKMAGGLPLIDLAGNKYDLTRPKEYFDSLISIAEKRMPAEARSFLD
                     VIKNEQFDWEAIIRESFNPAPEGEEIPEREARASGEHEEQFDLIDLFIEESLRPELEV
                     VAEKYGALVEKSDWTEGYCPICGKEPKIGEIRESEDGRRYLFCHQCGYKWHFRRIKCP
                     FCGNEEQHSLAYFAVEGEESHRVDVCNKCRRYIKIVELPKSSEGVNLDVEDIATLHLD
                     MLAYEEGYN"
     gene            complement(4459..5883)
                     /locus_tag="PLQ77_07240"
     CDS             complement(4459..5883)
                     /locus_tag="PLQ77_07240"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPL10508.1"
                     /translation="MKQKSWILLSVCTAWLFLPDLCIAAGPAGNIGAVLPLWSMLPFA
                     GILLSIALVPLVLPRFWHHHFPKVSAFWAIVFAVPFIYFFRETALYEIGHIMIVDYIP
                     FILLLWALFTVSGGIYVKGTLKGTPAVNVVILLIGTVLASLIGTTGASMLLIRPILRS
                     NAWRVYKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTLHILPETAFASV
                     ILLVLYFLLDSYYYRKESKTAVPEDESEPLSIEGKRNFIFLAGVIGAVLFSGSVKLGE
                     LSILGIHQTVENLIKDAILIIMGMLSLWLTAREVRKGNEFSWAPILEVAYLFAGIFIT
                     IIPALAILKAGEQGALHLLIRSVNTPADYFWAVGLLSSFLDNAPTYLTFFNSALGKFY
                     PGMPEAQAVAGLIVEKIPYLAAISAGAVFMGANTYIGNAPNFMVKSIAEEAGVKMPSF
                     FGYMFRYSIPILVVLFILITFIFF"
     gene            complement(5902..>6498)
                     /locus_tag="PLQ77_07245"
     CDS             complement(5902..>6498)
                     /locus_tag="PLQ77_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPL10509.1"
                     /translation="LDNLGVNITGLVAGLGIGGIAVALAVQNILGDLLASLSIVLDKP
                     FVIGDFIVVDTLSGTVEHIGLKTTRIRSISGEQLVFANNDLLKSRIRNYKRMQERRIV
                     FGFGVVYSTPLEKLTLIKKMVGDIVNATENARLDRIHFKEYGNSALNFEVVYFVAQPD
                     YGLYMDVQEAINLEIFRRFGQEGIEFAYPTQTIYIQNS"
BASE COUNT         1578 a         1844 c         1748 g         1328 t
ORIGIN      
        1 ggggaagaac gtccgtgatg tccggcaaca cggcggggtt cattctggag acggttcgcc
       61 gcaagaacat tgaagccaaa ggcggcgccg tcaacgagga ggatatggaa acattccgtc
      121 agatgatgcg ggaacgctac aacagcgaag gccatccctt ttacaccggc tcactgatgt
      181 tccacgacgg cgtgattgcc ttttccgaag cgcgcgacaa gctggcccgc gcttttgaaa
      241 tagccctgcg cacccctgtg agagagtccg attggggaga cctgaaattt taaccgttta
      301 ttgcaaggag taaagaatat ggcagatgtt ttattgcggg agcaaaaagg cgaagggata
      361 acgctgctga cgctgaaccg tccggaagcc atgaattgct ttaactttga cctgctgggc
      421 gcgctgggcg acgccgtgcg ggaatccaat ttcgattccg gcctgcgttg tctgattatt
      481 acgggggcgc ctgccgacaa ccccaaaaag gcctcttttt ccaccggcgc cgatctcatc
      541 gagcgccgga cgatgccgcc ggagcaggtc cgccgcttca tctttaccat ccgcaatctt
      601 ttcacgtccg tggagcagct gcgcgttccc gtcatcgctg ccgtcaatgg ttttgccttc
      661 ggcggaggga cggagcttgc gctggcctgc gacctgcgca tcgcttccag caacgtggtg
      721 atggggctta cggaaacctc actcgcgatc attccggggg ctggcggcac gcagcggctg
      781 ccgcgcattg tcggtctggc caaggccaaa gagctgatct ataccgcccg ccgcatcaac
      841 gcccagaccg cgctggacat cgggctggtc aaccgggtgg tggagccgga aaaactgatc
      901 gatgccgcct gcgaactggc ccgggaaatc gccgcgaacg gcccgattgc cgtggcgcag
      961 gccaaattcg ccatcaattg cggaatggac gcgcctctct cggtcgccct gccgctggaa
     1021 tcaaaggcgt acgaaatcac catcccgacc aaagaccggc tggaggcgct ggccgccttt
     1081 gccgaaaaga ggaaaccgga tttcaaaggt gagtaaaccg caaaaaatcg acaaaaggag
     1141 aataatgtat gaccgagtat gattattgga aaatttttcc ccggatgccg aagaaagcaa
     1201 ccattggaga tatcaccatt cgagacggtt tccagcattg tgaaaagttc atctccacgc
     1261 aggcgaaaat attctacgga caggaaatga tcctcgccgg ctgccgggag ctggaagtca
     1321 ccaacctggg aaaccccagg gccatgcccc agttcaagga cgcggacgaa gtcatgaagg
     1381 ccatgcgcgg cgacgacttc cggaaacgct gcggcaaagc cggcatcgat gtggacaaag
     1441 acgtcacgct cacctgcgtg accatccggg aagccgccgt ggaccgggcc attgaaatgc
     1501 gaaaaaacgg ctacggaccc gaccggatcc tgatgatggt ttcgaccgac ccggagcatc
     1561 atttcgccaa ctccggaacc acgctgagcc agtactggcg ggaagccgaa cgatgcattc
     1621 agaaggcgcg agacgcgggc atcaaaatgt gcggcacggt gagcaccatc tggggatctc
     1681 cgatcagcgg cgccaccaaa ctggaagacg cggtggaatt cacgaggcga tggttccaga
     1741 tcggagctca cgacatcgaa cacgccgatc acgacggctc cgccaacgca gccgatgcct
     1801 accggtattt ttccatggtg ttggacgcca tgcccaaccc ggaagcgcat ctgtgccatc
     1861 tccatgaaac caagcggatc gcctcttcca ccattctcgc ggccctgcag gccggcattt
     1921 gccgcttcga ggccacgctg gggggcttgg gcggccagcc ggccaatttc ctggatgact
     1981 gcccgatcaa gggcacgggg gaatattact acgatccgcg ctacgtcggc ctgatctgca
     2041 tggaggacct ggtcgtgcag atcgacgaac tgggcatccg gcacggatac gacgtcaacc
     2101 gcgtcctgat gctgggcaga aaaatggaaa agaccatcgg cgccagactg cgctccgaag
     2161 cgatcatcaa cggccgcacc gtcaaggaag ggcacatgga gcacgcgcgc cccggactga
     2221 aaaagctgat cgaaaaagaa aaggaaaaac cgggccagca gttcccggac gagtggagca
     2281 ccgaagcgat ccttcctgaa aaatgggggc cggcggatcc catctggaaa aagtaacccc
     2341 ctcaacaaaa aggacgcttg ccgcggcctt gcggcgagct tccttttttg ttagctttca
     2401 ttgtccgtca aaaattgcat ggaagcgcat gatgaaaaaa ataaaaatca ttgaagtagg
     2461 cccgcgcgac ggccttcaga acctcgagac gtttgtcccg acggacaaaa aaatcgccct
     2521 gatcaaaaaa ctggccggcg ccggcctcaa ggaaatccag atcggctctt tcgtctcccc
     2581 gcagtccatc gcccagttcc gggatattga ggaagtcgcc aaagccgtcc gccgtttgcg
     2641 gagcgtcgtg ccctcagcgc tggttccgaa tcggcgcggc gcggaaaagg ccctcgcctg
     2701 cggcatttcc aggctgattt tcttcttttc cgtgagttca aaacacaatt acgccaacgt
     2761 caagcaatcc ccgcaggatt ctttgaagga gctgaaaaac atctgccgga cctatacgga
     2821 cctggcttca acctctatcc gggtggacct ggccacggcc ttcggctgtc cgtttgcggg
     2881 caacgtcacg accgccgccg ttttgcgcat ggtggaaaaa gtcgcggcgc tgggcgttca
     2941 ggaaatcacg ctctgcgaca ccgtcggctt cggccatccg aaagccgtgg aagaaaaatt
     3001 ccgcaaatgc ttccggcttt ttcccgaaat ccgcttcggc gcccactttc acaacacgcg
     3061 cgggctggca caggccaatg ctctgcgcgc ctgggacatc ggaattcgct cctttgattc
     3121 ctcggtcggg gggctgggag gctgcccata cgcgcccgga tcttccggca acgtcgccac
     3181 ggaagacctg gtgtatctgt ttgaatcgat gggaatcaaa acgggaatgg atctgaaaaa
     3241 actcctgaac atctgcgcct ggctgaaaca aattttgcct caggccaaac tctccggctc
     3301 ccttttccaa gccggcctgc cccgcaccca caaactctcc tgctgaattg cttcttcggc
     3361 attctaaaga gattgcaaaa aaaatccctt tggtccggtc ccaaagggat ttttttcctt
     3421 cagccttccg ccttcggtct ttaagcctag ttgtagcctt cctcatacgc cagcatatcc
     3481 agatgcagcg tggcgatatc ttccacgtcc aggttcactc cctcggatga tttcggcagc
     3541 tcaacaattt taatgtagcg gcggcacttg ttgcagacgt ccacccggtg gctttcctct
     3601 ccttcaacgg caaaatacgc cagcgagtgc tgctcttcat tcccgcagaa ggggcatttg
     3661 atcctgcgga agtgccattt ataaccgcac tggtggcaga aaagataccg ccttccgtct
     3721 tcgctttccc tgatttcacc gattttcggc tccttgccgc agataggaca atagccttcc
     3781 gtccagtccg atttctccac cagggcgcca tacttttcgg cgacaacttc aagctccgga
     3841 cgaaggcttt cttcaatgaa caaatcgatc agatcaaact gttcttcatg ctcgccggat
     3901 gccctggctt ccctttccgg aatttcttcg ccttcggggg cgggattaaa agattcccga
     3961 atgatggcct cccagtcgaa ctgctcattt ttgatcacat ccagaaagct gcgtgcttcg
     4021 gcaggcattc tcttttccgc aatggaaatc agcgagtcaa aatattcttt cgggcgggtc
     4081 aaatcatact tgttccccgc aagatcaatc aggggaaggc ctcccgccat tttaccggga
     4141 atcaacttgt cttccacgga aaagatcggc tttgtcatat tcttgcggta ttcttcgcgc
     4201 agaataagga tatccccaag gatatcaagc agatccctgt agtgcggatt ggcggttttg
     4261 tattcctcaa tggtttttaa agattttctc agggttgacc ccataactgt cctccatgtt
     4321 gatctttttc cagttttatc attttcagcg acggtgcggt taagacaacg acggcgcttc
     4381 atgcaaagcg gatttcacct tatccgcttt gcacgtcatc ttcaagcaat atttatacgc
     4441 tcgcggattt gtcaaatatc aaaagaaaat aaaggtgatc aggatgaaca aaaccaccag
     4501 aatcggaatg gaataccgga acatgtatcc gaaaaagctg ggcattttga caccggcctc
     4561 ttccgcgatg gatttcacca tgaaattggg agcattgccg atgtaggtgt tggcgcccat
     4621 gaaaaccgca ccggcggaaa tggcggccag gtagggaatt ttttcaacga tcaggccggc
     4681 aaccgcctga gcttccggca tgccgggata aaatttgccc agcgcgctgt tgaaaaacgt
     4741 caaataagtc ggggcgttat ccagaaaact ggacaacagg cccaccgccc agaaatagtc
     4801 ggccggcgtg ttgaccgacc ggatcagcaa atgcagcgct ccctgttcgc ccgctttcag
     4861 aatcgccagc gccggaatga tggtgatgaa gatgccggca aacagatagg cgacttccaa
     4921 aatgggcgcc caactgaatt cgtttccttt gcggacctcc cgcgcggtca gccaaagcga
     4981 taacattccc atgatgataa ggattgcatc cttgatcaaa ttttccaccg tctggtgaat
     5041 gccgagaatg ctgagctcgc ccagtttgac ggacccgctg aacaggaccg ccccgatgac
     5101 accggccagg aaaataaaat tgcgtttccc ctcgatgctc agtggctccg actcatcttc
     5161 cggcacggcg gtcttgcttt cttttctgta atagtaggaa tccagaagaa aatacaaaac
     5221 cagaagaatc acgctggcaa acgccgtttc cggcagaatg tgcagcgtcc agaagaacgg
     5281 gacgccgtgc aggaatccca gaaaaagggg cggatccccc agcggcgtca gcgatccgcc
     5341 gatattgctg accagaaaga taaaaaatac aatggtgtgc accttataca cccgccaggc
     5401 gttcgatcgc aaaatgggcc ggatgagcag catggacgcg ccggtcgtgc cgattaacga
     5461 cgccagaacc gtcccgatga gcagaatcac gacattgacg gcgggagttc ccttcagcgt
     5521 tcctttgaca taaatgccgc ccgaaacggt gaacagggcc cacagaagaa gaatgaaggg
     5581 aatgtagtcc acgatcataa tgtggccgat ttcatacagg gcggtttcgc ggaaaaagta
     5641 aatgaacgga acggcaaaga caatcgccca gaaagcggaa accttgggga agtggtgatg
     5701 ccagaagcgg ggcagaacga gaggaacgag cgcaatcgag agcaaaatgc ccgcaaaggg
     5761 cagcatcgac cacaggggca gaacggcgcc gatattcccg gcgggcccgg cggcaataca
     5821 caaatccggc agaaaaagcc atgccgtgca aactgaaagc agaatccaac ttttttgttt
     5881 caaatcgcgc gggcaccttc tttaagaatt ttggatataa atcgtctgcg tcggataggc
     5941 aaattcaatg ccctcctgcc caaagcgccg gaaaatttcc agattgatcg cctcctgaac
     6001 atccatatag agcccgtaat ccggctgcgc cacaaaataa accacttcaa aattcagggc
     6061 ggaattgccg tattccttga aatgaatgcg atccaggcgg gcgttctccg ttgcgttcac
     6121 aatgtcgccg accatttttt tgatgagcgt cagcttttcc agcggggtgg aatacaccac
     6181 gccaaatccg aaaacaatgc ggcgctcctg catgcgcttg taattgcgga tgcggctctt
     6241 gagcaaatca ttgttggcaa agacgagctg ttcgccgctg atgctgcgaa tgcgcgtcgt
     6301 cttcaggccg atatgctcga ccgtgccgga gagcgtatcc acgacaatga aatcgccgat
     6361 gacaaagggc ttgtccagaa caatggaaag ggaggccagc agatcgccca ggatgttctg
     6421 caccgccagc gccacggcga tgccgccgat gccgaggccc gccaccaggc cggtgatgtt
     6481 gacgcccagg ttgtccag
//
LOCUS       DAOPAF010000083         6176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_11171_length_6176_cov_45.134782, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000083 DAOPAF010000000
VERSION     DAOPAF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11171_length_6176_cov_45.134782"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLQ77_07250"
     CDS             <1..328
                     /locus_tag="PLQ77_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPL10510.1"
                     /translation="TIPENLDSVLKESANIWFAEKTHLTVTVLVDSQVSHYFKRRKMF
                     PTQEIREERPDGSLVVVFRVGHFEAIRDILKSWIPHISILEPEEFRRKLLAEVKDWVA
                     KQTAAA"
     gene            546..1826
                     /locus_tag="PLQ77_07255"
     CDS             546..1826
                     /locus_tag="PLQ77_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPL10511.1"
                     /translation="MKNKNVFFKVFPGIALVVLFLVLIPVFPAAPAGAEKPTAISDAE
                     TDPAVWGKIYPLQYNSYLKSKEMAASPTGFGGSIKVQKSVMQPEMLMNFKGNAFSKDY
                     SEDRGHPYSMDDLKESKRVTPITPGSCMTCKTSNLRNIYKDMGWNYAKTPLAELLPVI
                     KHTIACANCHDPQGMQLRVSNPAFIEAMGKRGIDVAKAPRQDMRSYVCGQCHSEYFLE
                     PKTSRVIFPWDKGLKPQEIYAYYQNLPSGFEMDWQHPDSQARMLKAQHPDFELATNGF
                     HGKMGLACANCHMPSVQEGGVQYSSHWVTSPLKHIQASCRTCHQQDEPLILERVKTKQ
                     NDVFQLLHTAGQTIARAHEALGKAGAAPKVNKAELGKARELLRNAQWLWDFFAAENSM
                     GFHNADEAQGTLKQSNDLAKQAIGMARKAAGIKN"
     gene            complement(1906..1981)
                     /locus_tag="PLQ77_07260"
     tRNA            complement(1906..1981)
                     /locus_tag="PLQ77_07260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Ala,seq:cgc)
     gene            2247..3329
                     /locus_tag="PLQ77_07265"
     CDS             2247..3329
                     /locus_tag="PLQ77_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPL10512.1"
                     /translation="MAKIDAFFQLMHEQGASDLHLVSGQQPVIRMRGDLERIKYDVLT
                     NDGLKALLYEITPEHKIKVFEETGDVDFAYEIPGLARYRANFFQQKYGVAAVFREIPS
                     KILTCEQLALPPVLSKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRTRKDHIITVE
                     DPIEFVHQSQSCVVNHREVGVHTKTFSSALRGALREDPDIILVGELRDLETISLAVEA
                     ASTGHLVFGTLHTTSAPKTVDRIIEVFPSSEQAQIRSTLADGLRAVIAQVLFKRIDIK
                     GRCAALEILIATAAVRNLIREAKTFQIPSMMQTGKKYGMQLLDDAIMDYVNKGWISGE
                     EAYNKANDKAKFRPLLKSPPTDFTEA"
     gene            3348..4541
                     /locus_tag="PLQ77_07270"
     CDS             3348..4541
                     /locus_tag="PLQ77_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPL10513.1"
                     /translation="MRKQEIDRILEHMLASHPNISDLNLTVNKPFQVESSGQLTGVDL
                     DPPFARLTPFQTETFALNLVNQDHRLTDDLVSTGSCDLSYELPGIARFRVNVFSQRGH
                     YSIVLRKLETKIPSCADLKLPEAFYNISREKNGIVLVTGSTGSGKSTSLAAVINEINE
                     QKSIHIITLEDPVEFTHQQKKATINQRELGKDFDTFSSGLRAALRQAPKVVLVGEMRD
                     RESVEIALSAAETGHLVLSTLHTVDAGSTINRILGMFPSEEENQIRIRLADTVRWIVC
                     QRLLPKEGGGRVAAFEALGMNLRVKDIIQNGESEGKTFYDVMEQSKAFGMITFDSCIV
                     DLYSKGLISEETAKAYASNKSNVNRGIDAIKNARGEKTTDLGKLEVDKSYGRPQAGAP
                     PWQKK"
     gene            4580..5092
                     /locus_tag="PLQ77_07275"
     CDS             4580..5092
                     /locus_tag="PLQ77_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10514.1"
                     /translation="MSEENAAAASGTAREMSEERPFGFIAPEGNTAMVCEPDAAVREK
                     IVSELKGMGYAVAEAKSAREALQFMRFHIFSAIFVNESFSTAEGQENSVLKFLEGLSM
                     TVRRQTFVVLISASLRTMDNMMAYNRSVNLIVNQNEIAEVGQVFTKALDEHESFYHVY
                     KENLRKAGKA"
     gene            complement(5089..6027)
                     /locus_tag="PLQ77_07280"
     CDS             complement(5089..6027)
                     /locus_tag="PLQ77_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL10515.1"
                     /translation="MTTESSSQPILDEADRLIEEGKPGEAVALLEALHAREPDEEPVL
                     LRLAWASWDIGRKEQSIQYWEELLERELQRRIFTGFAYDELVRIYKQEALFEKLIAVC
                     EKAVAAQPDDAGLLAELGNAQLQSGRALEACRIFEKLTRMEEDNPDFLCRLGEALFAA
                     GRASESEAACLRAGRMDPEQIDRYSFRIADLYSRGKRHAEAIRLLERCIAANPSQPLY
                     YCSLGDELAALGRVDEAEAVYETAAAKSPSGAYYNRLGNTLMKEKMFDRAARAFEKAA
                     AREGLRPFFVNLARAYREMGLPEAAGEALRKAGETS"
BASE COUNT         1451 a         1729 c         1753 g         1243 t
ORIGIN      
        1 gaccattccc gagaatctgg attccgtcct taaggaaagc gccaatatct ggtttgctga
       61 aaaaacgcat ttgacggtga cggtcctggt ggattcccag gtcagccatt atttcaagcg
      121 ccgcaagatg tttccgacgc aggaaatccg ggaggaaagg ccggacgggt cgctggtggt
      181 cgtctttcgg gtgggccact ttgaggcgat tcgcgacatt ctgaagtcct ggataccgca
      241 catctcgatt ctggagcccg aagagtttcg gcggaagctg ctggctgaag tgaaagactg
      301 ggtggcaaag caaaccgcgg cggcttagtt ttgtccggcg gggagggtcg aactgacatt
      361 tcatcgccgc cggcaaaaaa aatttgctca ggctctttcg ctgtgtcata atgcgctcaa
      421 cattgagatc agtaaatcag agtaaataac ggcggattct tccgcagcgg aatggcgcga
      481 attgcatgaa gtgtcacggt gatccggttg ccaacgggag gcaaggcaaa gaaaggggaa
      541 tgaaagtgaa gaataaaaat gtatttttta aagtttttcc gggcattgct ttggttgtgc
      601 tgttccttgt cctgatccca gtatttccgg cagcgcccgc cggcgcggaa aaaccgacgg
      661 ccatctccga tgcggaaacc gatcccgccg tgtggggcaa aatctatccg ctgcaataca
      721 acagctattt gaaaagcaaa gaaatggcgg cttcgcccac cggattcggc ggctccatca
      781 aggtccagaa atccgtcatg cagccggaga tgctgatgaa cttcaaggga aatgccttca
      841 gcaaagatta ttcagaagac cgcggacatc cttattcaat ggatgattta aaggaatcga
      901 aaagagtaac ccccatcacg ccgggttcct gcatgacctg caaaacgtcc aatctgcgca
      961 atatttataa agacatgggc tggaattacg caaaaacgcc gcttgccgaa ctgctgcccg
     1021 tgatcaagca taccattgcc tgcgccaatt gtcatgatcc gcaaggcatg caactgcgcg
     1081 tcagcaatcc tgcctttatt gaagctatgg ggaagcgagg catcgacgtc gccaaagcgc
     1141 cgcgccagga tatgcgcagc tatgtgtgcg ggcaatgcca ttccgagtat ttccttgaac
     1201 ccaaaacctc gcgcgtgatt ttcccctggg ataaaggatt gaagccccag gaaatatacg
     1261 cctattacca gaaccttcca tccggatttg aaatggactg gcagcatccc gattctcagg
     1321 cgagaatgct caaagcccag catcccgatt tcgagttggc gacgaacggt tttcacggca
     1381 agatgggcct ggcatgcgct aattgccaca tgccttccgt gcaggaaggc ggagttcaat
     1441 actcatccca ctgggtgaca agcccgctga agcacattca agcctcctgc cgcacctgtc
     1501 atcaacagga cgaaccattg attctggaac gggtcaaaac aaagcaaaac gatgtttttc
     1561 aattgctgca cacagcgggg caaaccattg cccgcgcgca tgaggcgctc ggcaaagccg
     1621 gcgccgcccc gaaggtcaac aaggccgaac tgggcaaggc gcgtgaactc ctccgcaacg
     1681 ctcagtggct atgggatttt tttgccgcgg aaaactccat gggttttcat aatgccgatg
     1741 aggcgcaagg cacgctgaaa caatccaatg atctggcgaa gcaggccatt ggtatggcga
     1801 gaaaggcggc gggaataaaa aactaatttt gatgcgtcgc gcagcaaaaa cccgcggttg
     1861 acgcgggttt ttttgcactt ctttggattg tattggatta aggtttggtg gagatgagga
     1921 ggatcgaact cctggcctcg gcgttgcgaa cgccgcgctc tcccagctga gctacatccc
     1981 cgaaccgcaa aaacgcgcgg agcatatcta ttgtttgaat aagtgtcaag aattagttga
     2041 agggcggggc cgaggcgttc tgaacgcctt gcaacgcgcc cggttttccc aaacttttct
     2101 tgacaaaacc gaaccgtttt ccctatactc gcaaccgttc catggagcat cggaatcgcc
     2161 gtgcggacgg ggggatgagc cgccgggcag ggccgtccgg accatcaaac cataggataa
     2221 aataaaatta tcgacgggag acatttatgg caaagatcga cgcttttttt cagctcatgc
     2281 atgaacaggg tgcgtccgac ctccacctgg tttccgggca gcagcccgtc atcagaatgc
     2341 gcggagacct tgaacgaatc aagtacgatg ttctgaccaa cgacggcttg aaagccttgc
     2401 tgtatgaaat tacgccggag cacaagatca aggtgtttga agaaaccgga gacgttgact
     2461 ttgcctatga aattccgggg ctggcccgct accgggccaa cttttttcag cagaagtacg
     2521 gcgtcgccgc cgtcttccgt gaaattccca gcaagattct gacctgcgaa cagctggcac
     2581 ttcctccggt gctgtccaaa ctggcccaac tgccgcgcgg actggttctg gtgaccgggc
     2641 cgacgggctc cggcaaatcg acgacgctgg cggccatcat cgatgaagcc aaccgcaccc
     2701 gcaaggacca tatcataacg gtagaagatc cgattgaatt cgttcaccaa agccagagct
     2761 gcgtcgtcaa ccaccgcgaa gtcggcgtgc ataccaaaac gttcagctcg gcgctgcgcg
     2821 gggcgctgcg cgaggacccg gacatcattc tggtcggcga attgcgcgat ctggaaacca
     2881 tctccctggc cgtggaagcg gcgtccaccg gccatctggt attcggcacc ctgcacacga
     2941 ccagcgcgcc caaaaccgtg gaccgcatca ttgaggtgtt cccctccagc gaacaggcgc
     3001 agatccgctc cacgctggcc gacggcctca gggcggtcat cgcccaggtg ctgttcaaga
     3061 gaatcgacat caagggccgc tgcgccgcgc tggaaattct gatcgccacg gcggcggtgc
     3121 gcaacctgat ccgcgaggcg aaaaccttcc agattccgtc catgatgcag accggcaaaa
     3181 agtacggcat gcagcttctg gacgacgcga ttatggatta cgtcaacaaa gggtggatca
     3241 gcggcgaaga ggcctacaac aaggccaacg acaaggcaaa attccgtccg ctcctcaaaa
     3301 gtccgcccac ggactttacc gaagcataac gcaagcgagg tggatttatg agaaaacagg
     3361 aaatcgaccg tattttggag catatgctgg cgtcccatcc caatatttcc gatttgaacc
     3421 tgaccgtcaa caagcctttt caggtggagt cttccggcca gttgaccggc gtcgatctgg
     3481 acccgccctt tgcccggttg acgccgtttc aaacggaaac ctttgcattg aacctggtga
     3541 atcaggatca ccgcctgacc gacgatctgg tgtccacggg ctcctgcgac ttgtcctacg
     3601 aactgccggg aatcgcccgg ttccgtgtga atgttttctc ccagcgcggc cattactcga
     3661 tcgtccttcg gaaactggag accaagattc cgtcctgcgc ggacctgaag ctgccggagg
     3721 ccttttacaa tatctcgcgc gaaaaaaacg gcatcgttct ggtgaccggt tccaccggca
     3781 gcggaaaatc cacgtcgctg gccgccgtga tcaacgaaat caacgagcag aaatccatcc
     3841 acatcatcac gctggaagac ccggtggaat tcacgcatca gcagaagaaa gccaccatca
     3901 accagcggga gctgggcaag gatttcgaca cgttttccag cgggctgcgc gccgcgcttc
     3961 gtcaggcgcc caaggttgtt ctggtcggtg aaatgcgcga ccgcgaatcc gtagaaatcg
     4021 ccctgtccgc cgcggaaacc ggccacctgg tgctgagcac gctgcacacg gtggatgccg
     4081 gctccacgat caaccgtatt ctgggcatgt tcccctccga ggaagaaaat caaatccgca
     4141 ttcgtttggc cgacacggtc cgctggattg tctgccagcg cctcctgcct aaggagggcg
     4201 gagggcgggt ggccgctttt gaggcgctgg gaatgaattt gcgggtgaaa gacatcattc
     4261 aaaacggcga atccgaaggc aagacctttt acgacgtgat ggagcagagc aaggcgttcg
     4321 gaatgatcac gttcgacagc tgcatcgtgg atctctactc caaggggctg atttccgaag
     4381 aaacggccaa agcctatgcc tccaacaaat cgaacgtcaa ccggggcatt gacgccatca
     4441 agaacgcccg cggcgagaaa accacggatc tcgggaagct ggaggtggac aaatcctacg
     4501 gcaggcccca ggccggcgcc cctccctggc agaaaaaata aatgactttg ccaaagaata
     4561 tcgagcgaag gagatgatta tgtcagaaga aaacgcagcg gcggcatccg ggacggcgcg
     4621 ggagatgtcc gaggagcgtc ctttcggctt catcgcgccc gaagggaaca ccgccatggt
     4681 ttgtgagccg gacgccgccg tccgggaaaa aatcgtaagc gagctcaaag gcatgggata
     4741 cgcggtggcg gaagcgaagt ccgcccggga agccctgcaa ttcatgcggt ttcatatttt
     4801 cagcgccata ttcgtaaatg agagcttcag cacggccgaa ggtcaggaaa acagcgttct
     4861 gaaatttctg gaaggtttgt ccatgaccgt gcgccggcag accttcgtgg tgctgatcag
     4921 cgcgtcgctc aggaccatgg acaacatgat ggcctacaac cgaagcgtca acctgattgt
     4981 caatcaaaac gagatcgcgg aagtcggcca ggttttcaca aaagcgctgg acgagcacga
     5041 gagcttttat cacgtctata aggaaaattt gcgcaaggcg ggcaaggctt aagaggtttc
     5101 accggctttc cgaagcgcct ccccggctgc ttcgggcaga cccatttccc ggtaggcccg
     5161 cgccagattg acgaagaagg ggcgcaggcc ttcacgcgcg gcggcttttt caaaggcgcg
     5221 ggccgcgcgg tcgaacatct tttctttcat cagggtgttc cccaggcggt tgtagtaggc
     5281 gccggatggg cttttcgcag ccgcggtttc atagacggct tcggcttcat cgacgcgccc
     5341 cagcgcggcg agctcgtccc ccaggctgca gtagtaaaga ggctgagagg gattggcggc
     5401 aatgcaccgc tccagaagcc gtatcgcctc ggcgtggcgc tttccccggg aatacaaatc
     5461 cgcgatcctg aaactgtagc ggtcgatctg ctccggatcc atgcggccgg cccgcaggca
     5521 ggccgcttcg ctctccgatg cgcgcccggc ggcaaagagc gcttcgccca gacggcacag
     5581 aaaatcggga ttgtcttctt ccatccgggt gagcttttca aaaatgcggc aggcttccaa
     5641 tgctcttccc gactgaagct gtgcgtttcc cagttcggcc agcaggcccg cgtcgtccgg
     5701 ctgcgcagcg acggctttct cgcagacggc gatcagcttt tcgaacaggg cttcctgctt
     5761 gtaaatccgc acaagttcat cataggcaaa gccggtaaaa atcctgcgct gaagctcccg
     5821 ctccagcagc tcttcccagt attgaatgga ttgttccttg cgtccgatgt cccacgaggc
     5881 ccaggccagg cgaagcagta cgggctcctc gtccggttcg cgggcgtgaa gcgcctccag
     5941 aagggcgacg gcttcgccgg gttttccctc ctcgatcagg cggtcggctt catcgaggat
     6001 tggctggctg gaagattcgg tcgtcatggc gaaaaaaacg ggggagaaaa ttctcccccg
     6061 gaatctggtt cagcaactgc aggaaccgca gccgcttccc gcggcgggga ggttggacgt
     6121 taatttgaag cccgctccct gcggggttgt gatgaaatca atgttaatcg gttttg
//
LOCUS       DAOPAF010000084         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_11465_length_6048_cov_46.426831, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000084 DAOPAF010000000
VERSION     DAOPAF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11465_length_6048_cov_46.426831"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLQ77_07285"
     CDS             <1..193
                     /locus_tag="PLQ77_07285"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPL10516.1"
                     /translation="IHGVRTRRSGSHTFIEIFLEFASDAKVEEVYRLKDRLEAELKKQ
                     MPNAQIMLIPSRLTGNARF"
     gene            514..1428
                     /locus_tag="PLQ77_07290"
     CDS             514..1428
                     /locus_tag="PLQ77_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPL10517.1"
                     /translation="MMKNAQPLENRSRGEEALISDIKGPFLFPVPDIRRAENALSGED
                     NISQKIIDCFPGWFYLMDQQLNLVFWNDNFRQATGCSNEELRGMSVLEFYKEAQQAFI
                     AEELIRVFRLGEYAAEADIAFREGTVRTFYFNFKQVCYGNAPCLAAAAFDVSGWKGGH
                     VHFSRQPQNTSLSLQVSLRSEKEGRQATEDKLKANLEDLVIPHLKKLKATNLNATQRK
                     HLEALENRLNDVLSPFVNSLLLIYSNLTPQEIQIADLVRSGNGTKEIADTLNTSVNTI
                     ATHRNNIRKKLNLRNTKVNLRTHLQTIL"
     gene            1463..2287
                     /locus_tag="PLQ77_07295"
     CDS             1463..2287
                     /locus_tag="PLQ77_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10518.1"
                     /translation="MEALHQAIDFKPRYMEVTDSADAGRPVRKADPSHAVAAGTSHLF
                     NHHLVPALLNIEMAMNKLPKNGKTFRLLSDAMKSACQAAEICRMLMGSLGFCLGCGSS
                     GRAPVDVSGACLRFLPVFRALVLPNTRLEADLPPGGPSVRISRDLLNQVLINLIRRAG
                     EFFEGAPGVFYFRVRTAGPEELPMHAPFSHGSRSAYACVEIESRGCPSAERDGSAMER
                     TVSAAGSILSVYGGYAAAQTNEAGKVAFRIFLPVCKTEPPPSGTKVVSGSIAELFS"
     gene            complement(2339..2647)
                     /locus_tag="PLQ77_07300"
     CDS             complement(2339..2647)
                     /locus_tag="PLQ77_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10519.1"
                     /translation="MNISEWLDQKEAQGFDVSRAVLPEELEREEEPDETVYFKEIRPC
                     SVLCTGDHPFAAVERFGRWYRSRGKEKEKGPHTGKPQWWLFTRDKDLAIQTARSRIEK
                     "
     gene            complement(2672..2881)
                     /locus_tag="PLQ77_07305"
     CDS             complement(2672..2881)
                     /locus_tag="PLQ77_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10520.1"
                     /translation="MAENKKEGTLINCFFCRHFYLTGRVKSPYGCRAHGFTTFRMPSV
                     DVFEASDEDCALLDRREPDEHPHDG"
     gene            complement(2982..5960)
                     /locus_tag="PLQ77_07310"
     CDS             complement(2982..5960)
                     /locus_tag="PLQ77_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPL10521.1"
                     /translation="MTTIDVLQPTLKAGDVVHGFIVRRVEVIETMRLAAYEMEHRKTG
                     AKVLHLHAADRENLYVIGFRTPPSDSTGLPHILEHSVLAGSEKYPLRDVFKELMRSTM
                     QTFINAFTYPDKTLYPAASQIKTDFYNLARVYTDLVLHPRLLRETFLQEGHHLEFAKP
                     GDPESDLIISGIVYNEMKGAYSSPESLMYKDIQQNLYPDTVYARDSGGDPNVIPTLTY
                     EQFRDFHRKYYSPTNARFFLYGDIPTAEHLAFLAQMLDGFERVEVDSSIASQPFFDGP
                     RRVTGAYPIDPDEPGENKTHVNVAWLLAENRDFETALLLSILSGLLVGSAASPLRRAL
                     VDSGLGEDLSPVSGIESDLKQLMFCAGLRNAGSADIPAIEKLILDTLKKIVDEGFDPE
                     LIEGILHQVEFHGKEISRGSYPYGITLMGGVFQTWLYDGDPLIGLDFSRVIENIRRFL
                     KDDPQIFQKVAQKWLLDNPHRLLAVMEPDPDFNQKNEARLRKKMADLKAGLSKEQLRE
                     IDEQAALLKAFQSADDSSAAQACLPKLKMEDLPRRVETIPTRRATAVPVPALAHDLFT
                     NGIAYADLAFDTASVPEDLQMLLPLLGKIMSGMGAAGLTYDEMAKRIALQMGGFGYDL
                     SCGRSADAGCVWQKMIFSFKALYRNVPNACRTVRDILCLGDLNDESRMRDLIAERKNN
                     LLASVVPSGHVFAKRTAAAGLTLPAHRDEQWHGRTQLRFVQQQAENFDASKNLLREKL
                     DRLRKILFTRKNLTVNLTADADGLRLLEEQIGVLLESLPAGRLDEKAAPPALSPVRSG
                     IAVPAQVSYVASAMKAPAYNDPDSALLMLASRELSNTYLYQRIRVQGGAYGGMTSFDA
                     MQGVFAFLSYRDPHIVETMDVFEAAKRFYSENEMNHDDLEKAVISALGALDKPLDPSG
                     RGYAALLRNFSGVTDDMRQFFRDRLFAATPGQVRQALSAYFEAASPSGSVAVYSSQEK
                     LEEANQLLEKKLKLEKLSEG"
BASE COUNT         1264 a         1674 c         1796 g         1314 t
ORIGIN      
        1 gatccacggc gtccgcacgc gccgttcggg aagccatacc ttcatcgaga tctttctgga
       61 gtttgcgagc gatgcgaagg tggaagaggt ttaccgactg aaggaccggc tggaggcgga
      121 gctgaaaaag cagatgccca acgcccagat catgctgatt ccgagccgct tgactggaaa
      181 cgcaaggttt tgattttatt tgataaacaa taataaaacg aatcaaccct ttcagggaag
      241 gcgccctctg tttcaaatgc ggcggcgctc aggttctccc ggcttccggc gtttttcagc
      301 ccgggaaaag ctcatttgcc atgtatttct tatccataat aattccatga attttcattg
      361 aacttcccct ttgacaagta tttccataaa cggttctaat catcctgaaa ataccgtccg
      421 gcagtcgagc tttccggcgc gggccatcgg tacggtcagg ggttgccgga acgcatccag
      481 gaaaaaggaa ctttttcagg agagatcaga accatgatga aaaacgcaca acctttggaa
      541 aaccgaagcc gtggggagga agcgctcatt tccgacatca aaggaccgtt tctttttccg
      601 gttccggata tccgccgggc ggaaaatgca ttatccggag aagacaacat cagccaaaag
      661 atcatcgatt gttttcccgg atggttttac ctgatggatc agcaattaaa cctggtcttc
      721 tggaacgaca acttccggca ggcgaccggc tgttcaaatg aagagcttcg gggaatgtcg
      781 gttctggaat tttataaaga ggctcagcag gccttcatcg ccgaagaatt gatcagagtt
      841 tttcgtctgg gcgaatacgc cgccgaagcg gacatcgcat tcagggaagg gacggtccgg
      901 acgttttatt tcaatttcaa acaggtctgc tacggcaacg ccccctgcct cgcggcggcg
      961 gctttcgacg tttccgggtg gaaaggcggg catgtgcatt tttccaggca gccgcaaaac
     1021 accagtttgt ccctgcaggt gagcctccgc tccgaaaaag aagggcggca agccactgag
     1081 gataaactga aggccaacct ggaggatctg gtcattccgc atttgaagaa actgaaggcg
     1141 acgaacctga acgccacgca acgaaagcac ctggaggccc tggagaaccg tctgaatgat
     1201 gttttgtcac cgtttgtgaa cagtctgctg ctcatttatt caaatctcac ccctcaggaa
     1261 atccagattg ccgatctggt ccgcagcggc aacggcacca aagaaatcgc cgatacgctc
     1321 aacacgtcgg tgaacacgat tgcgacccat cgcaacaaca ttcggaaaaa attgaacctg
     1381 cggaacacga aagtcaacct tcggacccac ctgcagacca ttttataaaa gatcgcaaac
     1441 acgaaaagga acccggaagc ttatggaagc acttcatcag gcgattgatt tcaaaccaag
     1501 atacatggag gtcacagact ccgcggatgc cgggcggccg gttcggaaag ccgatccttc
     1561 ccatgccgtc gcggcgggca cgtcccatct cttcaaccat catcttgttc cggcgcttct
     1621 caatatcgag atggcgatga acaagctgcc gaagaacgga aaaactttcc ggctgctttc
     1681 cgacgccatg aaatccgcct gccaggcggc ggagatctgc cgaatgctga tgggcagtct
     1741 gggcttttgt ctgggctgcg ggtcatccgg tcgggcgccg gtggacgtgt ccggggcctg
     1801 tttgcggttt ctgcccgttt tccgggcgct ggttttgccc aacacgcggc ttgaagccga
     1861 tttgccaccg ggcggaccgt cggtccggat cagcagggat ttgctgaacc aggttttgat
     1921 caacctgatt cgaagggcgg gggaattctt tgaaggagcg cccggcgttt tttattttcg
     1981 cgtgagaacg gccggaccgg aagaacttcc gatgcatgcc ccgttttcgc acggctcgcg
     2041 gtccgcttac gcgtgcgttg aaattgaaag caggggatgt ccttcggcgg agcgggacgg
     2101 gagtgccatg gaaagaaccg tgtccgcggc cggttccatc ttgtccgtgt acgggggata
     2161 tgcggcggcg caaaccaatg aggcgggcaa agtcgccttc agaatctttt tgccggtctg
     2221 caaaaccgaa ccgccgcctt ccgggacaaa ggtcgtttcg ggaagcatcg cggaattgtt
     2281 ctcgtaaacg gggatccgca gattccgacg tcagtccggg ttcgcccggg ggcggtcttt
     2341 acttttcgat gcgggacctg gccgtttgaa tggcgagatc tttatcccgg gtgaatagcc
     2401 accactgcgg tttcccggtg tgaggtcctt tctccttttc ctttccccgg ctgcggtacc
     2461 agcggccgaa tcgttcgacc gcggcaaagg gatgatcgcc cgtgcacaaa acgctgcagg
     2521 ggcggatttc cttaaagtaa accgtttcat ccggttcttc ctcgcgctcc agctcttcgg
     2581 gcaggaccgc gcgtgacacg tcgaatccct gagcttcttt ttgatccagc cactccgaaa
     2641 tgttcatatt cttcttcctt tcccgaaggc gttatccgtc gtgagggtgt tcatccggtt
     2701 ccctgcggtc cagaagcgcg cagtcctcgt ccgaagcttc aaacacatcc acggagggca
     2761 tgcggaaggt tgtgaaaccg tgcgcccggc agccgtaggg agactttacc cggccggtca
     2821 ggtagaaatg ccggcagaaa aagcagttga tcagggttcc ttcttttttg ttttccgcca
     2881 ttgaaaagtt ccgatgaaat gctgaaggcc agaggctgta gacgataggt gtgcattatt
     2941 tgtgatctct agcctatcgc caatggccca tagcctgttt tctatccttc cgacaatttt
     3001 tccagcttca gctttttttc gagaagctga ttggcctctt ccagtttttc ctgcgaggaa
     3061 taaaccgcga cggagccgga gggcgaagcg gcttcaaaat aggcggacaa tgcctggcgc
     3121 acctgcccgg gcgtcgcggc aaacagccgg tcccggaaaa actggcgcat gtcgtcggtc
     3181 acgccgctga aattcctcag cagggcggca tacccgcgcc ccgaaggatc gaggggcttg
     3241 tccagcgcgc cgagcgcgct gatcacggcc ttttccagat cgtcatgatt catttcattt
     3301 tcggaataaa accgcttggc ggcctcaaag acatccatcg tttccacgat gtgcgggtct
     3361 cgataggaca gaaacgcgaa cacgccctgc atcgcgtcga aagaagtcat ccccccgtag
     3421 gcgccgccct gtacgcggat gcgctggtac agatacgtgt tggaaagctc gcgcgaggcc
     3481 agcattaaaa gggcggagtc cggatcattg taggccggcg ccttcatcgc cgaggccacg
     3541 taggaaacct gcgcggggac ggcgatgccg gagcggacgg gcgaaagcgc gggcggcgcg
     3601 gctttttcat caaggcgtcc tgcgggcaga ctttccagca atacgccgat ttgctcttcc
     3661 agcagcctga ggccgtccgc gtcggcggtc aggttgacgg tcagattctt ccgggtgaag
     3721 agaattttcc tgagccggtc cagtttttcc cgcaggaggt tcttggaggc gtcgaaattt
     3781 tccgcctgct gctggacaaa gcgaagctgc gtccggccgt gccactgttc atcgcggtgc
     3841 gcgggcagcg tcagccccgc cgccgccgtc cgtttggcga agacatgtcc ggagggaacg
     3901 acggaggcga gcaggttgtt tttgcgctcg gcgatcaagt cgcgcattct cgactcatcg
     3961 ttcaggtctc caaggcagag aatgtcgcgg acggtccggc aggcatttgg aacattgcgg
     4021 taaagcgctt tgaaggaaaa aatcattttc tgccagacgc agcccgcgtc ggcggaccgg
     4081 ccgcaggata aatcgtagcc gaaaccgccc atttgcaggg cgatgcgctt ggccatctca
     4141 tcgtacgtca gcccagccgc gcccatgccg ctcatgattt tgcccagaag cggcagaagc
     4201 atctgcaggt cctcgggaac cgaggcggtg tcaaaggcca ggtcggcgta agcgatgccg
     4261 ttggtgaaca ggtcatgcgc gagcgccgga accggaaccg cggttgcgcg ccgggtcgga
     4321 atcgtttcca cccgccgggg aaggtcttcc attttcagct tgggcagaca cgcctgcgcc
     4381 gcggaagaat catccgccga ctgaaaggcc ttgagaagcg ccgcctgctc gtcgatttcc
     4441 ctgagttgct ctttggacag gccggccttg agatcagcca ttttctttcg cagccgcgct
     4501 tcattctttt ggttgaaatc gggatccggc tccatcaccg ccagcaggcg gtgaggattg
     4561 tccagcagcc acttttgagc cactttctgg aagatttgcg gatcgtcctt caggaagcgg
     4621 cggatgtttt caatcacgcg cgaaaaatcg aggccgatca gggggtcgcc gtcgtagagc
     4681 caggtctgaa aaacgccgcc catcagcgtg atcccgtaag ggtaggagcc gcgggaaatt
     4741 tccttgccgt gaaactcgac ctggtgcagg atgccttcga tgagctccgg atcgaaccct
     4801 tcgtcgacga tttttttcag ggtgtcgagg atcagcttct cgatggcggg aatgtctgcg
     4861 cttccggcgt tgcgcagacc cgcgcagaac atcagttgct tgagatcgga ttcgatgccg
     4921 gacaccggcg agaggtcttc ccccagcccc gaatcgacga gcgcccggcg cagcggcgag
     4981 gcggcgctgc ccacgagcag gccggagagg atggagagca ggagcgccgt ttcgaagtcc
     5041 ctgttttccg caagcagcca ggctacgttg acgtgggttt tgttttcccc cggctcgtcc
     5101 ggatcgatgg gataggcgcc ggtcacccgc cgggggccgt caaagaaagg ctggctcgcg
     5161 atggacgaat ccacctccac ccgttcaaac ccgtcgagca tctgagccag aaacgccaaa
     5221 tgctccgcgg tgggaatgtc gccgtagagg aagaagcgcg cgttggtcgg ggagtagtat
     5281 ttgcggtgga aatcccgaaa ctgttcatac gtgagcgtgg gaatcacgtt cgggtctccc
     5341 ccggaatccc gcgcatacac ggtgtccggg taaagattct gctgaatgtc cttgtacatc
     5401 agtgattcgg gcgacgagta ggcgcccttc atctcattgt aaacgatgcc ggagatgata
     5461 aggtcgcttt cgggatcgcc gggctttgcg aattccaggt gatgcccttc ctgcagaaag
     5521 gtttcgcgaa gcaggcgggg gtgcaggacc aggtccgtgt aaacgcgcgc cagattgtag
     5581 aaatccgtct tgatctggct ggccgccggg taaagggttt tgtccgggta ggtgaaggcg
     5641 ttgatgaagg tctgcatggt ggagcgcatc agctccttga agacgtcccg gagcggatat
     5701 ttctccgagc cggccaaaac ggaatgctcc aggatgtggg gcaggcccgt ggaatccgaa
     5761 ggcggcgtcc gaaagccgat gacgtaaaga ttttcccggt cggcggcgtg caggtgcagc
     5821 actttggcgc ccgttttccg gtgctccatc tcataggcgg caagccgcat cgtttcgatg
     5881 acttcgacgc gccttacgat aaagccgtgg acgacatcgc ctgccttcag cgttggctgc
     5941 aatacatcga tggttgtcat ttccggacgc tccttgccgt tgggggatta tggccgcgga
     6001 caatcctgaa tcgtcacgcg gcggttttgc gcctggcctt ccggcgtg
//
LOCUS       DAOPAF010000085         5521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_12650_length_5521_cov_44.548847, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000085 DAOPAF010000000
VERSION     DAOPAF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12650_length_5521_cov_44.548847"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLQ77_07315"
     CDS             <1..258
                     /locus_tag="PLQ77_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPL10522.1"
                     /translation="LRDQGVAVLICGAISRPMQMAIASLGIAVYPFIRGTVEEVLSAY
                     QSNRLGQAVFSLPGCHGRGNGGNGRGRGMRCRWRQTGGHER"
     gene            286..834
                     /locus_tag="PLQ77_07320"
     CDS             286..834
                     /locus_tag="PLQ77_07320"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPL10523.1"
                     /translation="MNSSIPKELFFTKGVGTHREQLHSFELALRNAGIEKCNLVQVSS
                     IMPPGCKIISRTEGLKRLRPGAITFCVMSRCCSNEPKRLISAAVGCAIPVDKKTYGYI
                     SEHHAFGFTEKKTGDYAEDLAAAMLASTLGIDFDVDESWDEKKELFRISGKIVGTRNT
                     TQSAVLKTADYTTVLAAAVFVL"
     gene            909..1142
                     /locus_tag="PLQ77_07325"
     CDS             909..1142
                     /locus_tag="PLQ77_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10524.1"
                     /translation="MNGRNQGGRGTGGGQGQGGGMGQGRGGGGRGRMGGPSAGGAVGT
                     CRCPQCGHTEPHERGVPCTQVKCPQCGTSMFRE"
     gene            1161..1523
                     /locus_tag="PLQ77_07330"
     CDS             1161..1523
                     /locus_tag="PLQ77_07330"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPL10525.1"
                     /translation="MPGGDATGPMGMGPMTGRAAGFCAGYSVPGYVNNGPGRAFGMGF
                     GRGAGFRRGFHAGGFGRRNRFYAGGAPVGAWAGGWNAPAAAPDPENEKQALKNQAEYL
                     QMQLDAVKKRLEELSAQS"
     gene            1601..1963
                     /locus_tag="PLQ77_07335"
     CDS             1601..1963
                     /locus_tag="PLQ77_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPL10526.1"
                     /translation="MKIAFTTAGNDVSAPLDSRFGRAPKFLIYDLDTGTFTLADNRQN
                     LNAAQGAGIQSAQNIARLGVSALVSGHCGPKAFRVLQAAGIKIYNSSAATVQEALDLF
                     RAGKLNEASSADVEGHWV"
     gene            1960..2451
                     /gene="tsaA"
                     /locus_tag="PLQ77_07340"
     CDS             1960..2451
                     /gene="tsaA"
                     /locus_tag="PLQ77_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPL10527.1"
                     /translation="MKNSRIEHRPIGVIHSEHTAAEKTPIQPVYAKGCKGRVEVYPEF
                     AEGLKDLGGFSHLYLLYHFHQCRKVQLTVKPFLQDVERGVFSTRAPCRPNPIGLSVVE
                     LIKMEGNILHVDGLDILDGTPLLDIKPYTEKFDLHAVKKNGWQDEVDEPTARIRGKRG
                     FRS"
     gene            2448..>2731
                     /locus_tag="PLQ77_07345"
     CDS             2448..>2731
                     /locus_tag="PLQ77_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPL10528.1"
                     /translation="MNMCLDCIPCFIRQAADTVRLSAPSEEDQNRLMHSVLQWLGNID
                     LRQAPPAAAQMIHRHLRSLLPTEDPYRASKDRHNQLAARLIPSIRRRIEAS"
     assembly_gap    2732..2831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2832..3326
                     /locus_tag="PLQ77_07350"
     CDS             <2832..3326
                     /locus_tag="PLQ77_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10529.1"
                     /translation="EVSAALFWIFHPLHVLLSALVTTAMYRRHSRSGIGRVFLIGYAG
                     SIGIATLSDSLIPFAGEWLLNLPNRGVHLGFIEKWWLVNPLAFAGIGLGFVVFHTQIP
                     HAGHVLLSTWASLFHIMMSLGSSIDVVTTILIAVFLFLAVWVPCCTSDIIFPLMWAKK
                     EEAA"
     gene            3323..4183
                     /locus_tag="PLQ77_07355"
     CDS             3323..4183
                     /locus_tag="PLQ77_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL10530.1"
                     /translation="MRISVASGKGGTGKTTVSVNLARVFGSDVLLADCDVEEPNAHLF
                     LKTSGPAEKVVGIPVPVVNETLCDGCGECSKICQFSAIVTFGTTPLVFPEMCHGCGGC
                     TLVCPPRAITEKDRRIGVVETSRAGHITLVTGRLDVGVALAPPLIRAVKAELPAAADA
                     ILDAPPGTSCPVIAVVRDTDMVLLVTEPTPFGMHDLTLAVDMVRELDLPFGVIVNRMG
                     SGDDRVQHFCEKEGIPILLTIPDDRRIAENYSRGILMVDALPEYKEIFKALLQKIRAQ
                     IGCGSAPCGD"
     gene            4186..5139
                     /locus_tag="PLQ77_07360"
     CDS             4186..5139
                     /locus_tag="PLQ77_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL10531.1"
                     /translation="MHSGPFEYLYGPVPSRRLGRSLGIDLVPHKTCTYDCIYCQLGNT
                     THKTVERRDYVPVEGVLSELKEKLAAGVSCDYITLAGSGEPTLHASLGELIRKIKEMT
                     EIPVSVITNGSLLELPEVREALFRADLVVPSLDAGDPAMFEYVNRPHRDIVFERMIEG
                     LVTFGKRYPGRLWMEVLLVSGVTGLASEVKKIASWIEKFPVEKIQLNTVNRPSYEDFA
                     CAVDPKQMKTLAGLFAGAVEILENAPPTVGASETTSDTTDADICNLLARRPCTLEGVA
                     LGLNLHPHDAAKRIERLIGDKTVTAKRNGGKIYYTSAERTP"
     gene            5136..>5521
                     /locus_tag="PLQ77_07365"
     CDS             5136..>5521
                     /locus_tag="PLQ77_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPL10532.1"
                     /translation="MKELVIISGKGGTGKTSVTASLAVLAGRPVVCDCDVDAADLHLV
                     LAPEIREEHEFRSGHQAVINRDHCADCGACADLCRFGAIREEEKEDSDTLFVVDAVSC
                     EGCGVCVHFCPEKAIDFPESLCGKWFV"
BASE COUNT         1176 a         1549 c         1636 g         1060 t
ORIGIN      
        1 cttcgggacc agggcgttgc ggttttgatc tgcggcgcga tctcccgacc catgcagatg
       61 gccatcgcct cgctgggcat cgcggtttat ccgtttattc gcggcacggt ggaagaggtt
      121 ctttccgcct atcaaagcaa ccggctgggg caggctgttt tttccctgcc gggctgccac
      181 ggccgcggaa acggaggtaa cggcagagga cgaggcatgc gctgccgctg gagacaaacc
      241 ggcggccatg aacgataaag cacaactcaa aaggagatta aaaaattgaa ttcatccatc
      301 ccgaaagaac tgttctttac caaaggcgtc ggcacgcacc gcgaacagct tcactcgttt
      361 gaactggcgc ttcgcaatgc gggcatcgaa aaatgcaatc tggtgcaggt ctccagcatt
      421 atgccgcccg gctgcaaaat tatttcccgg acggagggcc tgaaaaggct gcgacccggt
      481 gcgatcacgt tttgcgtgat gagccgctgc tgctcgaacg agcccaaaag gctgatttca
      541 gcggcggtcg gctgcgcgat tcccgtggac aagaaaacct acggctacat cagcgagcat
      601 cacgccttcg gctttacgga aaagaaaacg ggggactacg ccgaagacct ggccgcggcg
      661 atgcttgcct ccacgctggg catcgacttt gacgtcgatg aaagctggga cgaaaagaaa
      721 gagttattca gaatcagcgg gaaaatcgtg ggaacccgga acacaacaca gtccgccgtc
      781 ctgaaaaccg ccgactacac gacggtgctg gccgcggcgg ttttcgtgct ctgaccgtac
      841 gatcatccgg atcgagggcc atgaataaaa acacaacaaa ccggtaaaaa ccggaaggag
      901 gaaaacccat gaacggaaga aatcagggag gcagaggaac gggcggaggc cagggccaag
      961 gaggcggcat gggccaggga cgcggaggcg gcggacgagg ccgtatgggc ggtccgtcag
     1021 ccggtggagc ggtcggaacc tgccgttgcc cccaatgcgg ccacaccgag ccgcacgagc
     1081 gaggcgtgcc ctgtacgcag gtcaaatgcc ctcagtgcgg cacgtccatg tttagagaat
     1141 aagaagggag gaaaagtctt atgcctggag gagatgcaac aggcccgatg ggtatgggcc
     1201 caatgacggg aagagcggct ggattttgcg cgggctattc cgtgccgggt tatgtaaaca
     1261 acggtcccgg aagagcgttc ggcatgggat ttggaagggg tgcgggcttt cgacggggct
     1321 ttcacgcagg cggctttggc cggcgcaacc gtttttacgc gggcggcgcg ccggtaggag
     1381 cctgggccgg cgggtggaac gcgccggcgg ctgcgccgga tcctgaaaat gaaaaacagg
     1441 cgctcaaaaa ccaggcggag tatcttcaga tgcaactgga cgcggtcaaa aagcgccttg
     1501 aagaactttc agcccaaagt tagtatccat catccggatg ggcgccgccc ccgggccgcc
     1561 cgtccggatg atgaaagatg caaaaaagag gaaggaaaac atgaaaatcg cattcacaac
     1621 cgccggaaat gacgtatcgg cgccgcttga cagccgcttc ggccgcgcgc cgaaattttt
     1681 gatttacgac cttgacaccg gcacattcac cctggcggac aaccggcaga atctgaacgc
     1741 ggcgcagggc gcgggcattc agtcggcgca gaacatcgcg cgcctgggcg tgtcggcgct
     1801 ggtcagcgga cactgcggcc ccaaagcctt tcgcgtgctt caggcggcgg gcatcaaaat
     1861 ctacaactcc tccgccgcga ccgtacaaga ggcgctggat ctgttcaggg ccggaaagct
     1921 caatgaagca tcctccgccg acgtggaagg tcactgggta tgaaaaattc gcggatagag
     1981 caccggccga tcggcgtcat ccacagcgaa catacggccg cggagaaaac ccccattcag
     2041 cccgtgtatg ccaaaggctg caagggacgc gtggaagtgt atcccgaatt tgccgaaggc
     2101 ttaaaagatc tgggagggtt ttcgcatctt tatctgctgt atcattttca ccaatgccgc
     2161 aaggttcagt taaccgttaa gcctttcctg caggatgtgg agcgaggcgt gttttcaacc
     2221 cgcgcgccct gccgccccaa tcccatcggc ttgagcgttg tcgagcttat caaaatggaa
     2281 ggcaacattc tgcatgtcga tggactggat atccttgacg gtacgccgct tttggacatc
     2341 aagccctaca cggaaaagtt cgacctgcac gccgtgaaga aaaacggctg gcaggacgaa
     2401 gtggacgaac ccacagcccg cattcgggga aagagaggtt ttcgatcatg aacatgtgtc
     2461 tggattgcat tccctgcttc attcgccagg ccgctgacac agtccgcctg tcggctccgt
     2521 ccgaggaaga tcagaaccgt ttgatgcaca gcgtcctgca gtggctcgga aacatcgacc
     2581 tgcggcaggc gccgccggcc gccgcgcaaa tgattcacag acacctgcgc agccttttgc
     2641 ccacggagga tccctaccgg gcttccaagg accgccacaa tcaactggcg gccaggctga
     2701 tcccctccat ccggcggcgc atcgaagcct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn ngaagtatcg gccgcgctct tctggatttt tcatccgctg cacgtgctgt
     2881 tgagcgcgct cgtgaccacc gccatgtacc ggcgtcattc ccggagcggt atcgggcgcg
     2941 tgttcctgat cggttatgcc ggttccatcg gcatcgccac cctgagcgac tcgctcattc
     3001 ccttcgccgg cgaatggctg ttgaacctgc cgaaccgcgg cgtgcatttg ggttttatcg
     3061 aaaaatggtg gctggtcaat cccttggcgt ttgcgggcat cggactgggc ttcgttgttt
     3121 tccacaccca gattccgcat gccgggcacg tgctgctcag cacctgggcg tccctttttc
     3181 acatcatgat gtcgctgggc tcatccatcg acgtcgtcac caccattctc atcgccgtct
     3241 ttttgttttt ggccgtctgg gttccctgct gcaccagtga tattattttc ccgctgatgt
     3301 gggcaaagaa ggaggaagcg gcatgagaat atccgttgcc tccggaaaag gcggaacggg
     3361 gaaaacgacc gtcagcgtca atctggcacg cgtatttgga agcgatgtgc tgctggcgga
     3421 ctgtgacgtg gaagagccca atgcgcatct gtttttaaaa acctccggac cggcggaaaa
     3481 agtggtcggc attcccgttc ccgttgtgaa cgagacgctc tgcgacggct gcggcgaatg
     3541 ctcgaaaatc tgccagttct ccgcgatcgt gaccttcggt acaacccccc ttgtttttcc
     3601 ggaaatgtgc cacggctgcg gcggctgcac gctggtatgc ccgcccagag cgattaccga
     3661 aaaggaccgt cgcatcggcg tcgtcgaaac ctcccgcgcg ggccatatca cactggtcac
     3721 gggcagactc gacgtgggcg tggcgctggc cccgccgctc atccgcgccg tgaaagccga
     3781 acttcccgcc gcggccgacg ccatcctgga cgctccgccc ggcacgtcat gcccggtgat
     3841 cgccgtcgtc cgggatacgg acatggtgct tctggttacc gaacccacgc ctttcggcat
     3901 gcatgacctg acactggccg tggatatggt gagggagctt gaccttccct tcggcgtcat
     3961 cgtcaaccga atgggcagcg gagacgatcg cgtgcagcat ttttgcgaaa aagaagggat
     4021 tcccatcctg cttacgattc ccgacgaccg gcggatcgcg gaaaactatt cgcggggcat
     4081 cctgatggtc gacgccctgc cggagtacaa agaaattttc aaggcgctgc ttcaaaaaat
     4141 ccgcgcgcag atcggctgcg gatcagcccc ttgcggagat taaaaatgca ttccggtcca
     4201 tttgagtatc tgtatggccc cgtcccgtcc cgaagactcg gacgttcgct ggggatagac
     4261 ctggtgccgc ataaaacctg cacctatgac tgcatctact gccagctggg caacacgacg
     4321 cataaaaccg ttgaaaggcg ggattatgtt cccgtggagg gcgttctttc cgagctgaag
     4381 gaaaagctgg ccgccggtgt ttcctgcgac tatatcacgc tcgccggttc gggtgagccg
     4441 acgctgcacg cgtctctggg cgaactgatc cgcaagatca aggaaatgac ggagattccc
     4501 gtttcggtga tcaccaacgg ttctttgctt gagctgccgg aggtgcgtga agcgcttttc
     4561 cgggcggatc tcgtcgtccc ttcactcgac gcgggagacc cggccatgtt tgaatatgtc
     4621 aaccgcccgc accgggatat tgtatttgaa cggatgatcg aaggccttgt aacgttcgga
     4681 aaacggtacc cgggtcgtct gtggatggaa gtgttgctgg tttccggcgt cacggggctg
     4741 gcttcggaag tcaaaaaaat cgcgtcatgg attgaaaaat tccccgtgga aaaaattcag
     4801 ctcaataccg tcaaccggcc gtcctacgag gatttcgcct gcgccgtgga cccgaagcaa
     4861 atgaaaaccc tggccggtct ttttgccggc gcggttgaaa ttctagagaa cgcgccgccg
     4921 accgtcggcg catcggaaac aacatcggac acaacggatg cggatatttg caatttactg
     4981 gcccggcggc cctgtacgct ggaaggcgtt gctctgggcc tcaatctcca tccgcatgac
     5041 gcggccaagc gcatcgaacg gctgatcggc gacaaaaccg tcacggcaaa acgaaacgga
     5101 ggaaagattt attatacttc ggcggagcga acaccatgaa agaactggtc atcatcagcg
     5161 gcaagggagg gacgggaaaa accagcgtca cggcgtctct ggccgttctg gccggacgtc
     5221 cggtcgtgtg cgactgcgac gtggacgcgg cggacctgca cctggtcctg gcgcctgaaa
     5281 tccgggagga gcatgaattc cgcagcggcc atcaggccgt gatcaaccgg gatcactgtg
     5341 cggactgcgg cgcctgcgcg gacctgtgcc ggttcggggc gatccgggaa gaagaaaagg
     5401 aggacagcga tacgcttttt gtcgtcgatg ccgtatcctg cgaaggctgc ggcgtgtgcg
     5461 ttcatttttg ccccgaaaaa gccattgact ttcccgagag tctctgtggt aaatggttcg
     5521 t
//
LOCUS       DAOPAF010000086         5402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_12961_length_5402_cov_47.538433, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000086 DAOPAF010000000
VERSION     DAOPAF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12961_length_5402_cov_47.538433"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..616)
                     /locus_tag="PLQ77_07370"
     CDS             complement(62..616)
                     /locus_tag="PLQ77_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPL10533.1"
                     /translation="MNDQNQVIIDSAMSFAEAIEGTTAPMEVIDSLSMIDVLYYSFDG
                     QRHQGQILVDGRLEDDVYEIFLLIEKLKLPIGKVIPIVAYKWDDEASMADNNSSSFNF
                     RVIADTNKLSLHSFGRAVDINPFQNPVIYPTGLIAPEGAVYRPKDRGAFFASHPIVQE
                     FLRRGWHWGGNFEQPKDYHHFEKT"
     gene            complement(677..3247)
                     /locus_tag="PLQ77_07375"
     CDS             complement(677..3247)
                     /locus_tag="PLQ77_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPL10534.1"
                     /translation="MKSSVYQWGRLLLWIAADETQAEEQWPFAGGPFRPLKRPSVFFK
                     NLPSDYLLALDAEKSVSCGPAELRRLLALARDTRAGMIYSDYYEKNGSRFAGRPLNDY
                     QAGSLRDHFHFGPFFILSGAAVSSAVRKYGALPADPDSAFYDLRLRISLEHELIRVPE
                     FLYTVSAKEKMPSKKTGRKSEAHFSYVARENLIRQKQLEKIATRHLRRLGAWLPPRPA
                     ADRQKEEILWKASIVIPVFNRRKTIADALQSALAQQTDFPFNILVVDNHSTDGTTKLL
                     KTFAAQYPHVHHLVPSRHDLGIGGCWNEAIYSPLCGRYAVQLDSDDLYASPHTLQIMV
                     NELRRGPCAMAVGSYTLVNEHLKKIPPGLIDHREWTARNGHNNLLRVDGIGAPRAFDT
                     AILRRIGFPNVSYGEDYAAALRLSREYRIGRVYESVYLCRRWKDNTDAGLSVEKQNRN
                     DFYKDRLRTLEIKARQQINEKEKSTENINDKNQKECCHSRAGGNPKFLNTGFRVALRL
                     PGMTNEAVAAPPQTADRQTGSSVSLAPNQIFAEFPEGGSQTLHALCRALVESQKKSWP
                     AFAAACRDLASVQTRELSLGDYSVTLQYNPARTVSSGAAVDAESIRKRPCFLCAENRP
                     EEQSGILYRGDYQILCNPAPIFEHHLTVAALTHLPQDIRLSLDAFLDIAHDAARDYAV
                     FYNGPACGASAPDHLHFQMIPARALPFLNELKEGSFPIAPSAILHRPAKNCDRRVLLF
                     KSDHAGLLKEHMTRFLDAARKQLATQEEPLLNVFCTYGKHGWTITVFLRRKHRPDAYF
                     APGKSGIFVSPGAIDMAGVVITPRLADFERLTGNAIRSIYREVSLEGDTLNPIIEALL
                     "
     gene            complement(3264..3797)
                     /locus_tag="PLQ77_07380"
     CDS             complement(3264..3797)
                     /locus_tag="PLQ77_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPL10535.1"
                     /translation="MKKPGVLSAIRIGFLCLSCFCLIGCGYGFAPQGEHIDKRIVNIY
                     VAPFVNKTAEAEAENILRAAFIDQILQSSRFKSALTLEQADAVINGTVISITTGVLSY
                     RNNILAAEERMTITLEASLREKDSGKTIWSSQNVAGTSDYKLQDSVNPKPARQQALAK
                     LAKDTAENTFNLMMSDF"
     gene            complement(3808..>5402)
                     /gene="leuS"
                     /locus_tag="PLQ77_07385"
     CDS             complement(3808..>5402)
                     /gene="leuS"
                     /locus_tag="PLQ77_07385"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPL10536.1"
                     /translation="DYGTGCVMAVPTHDQRDFEFAKKFNLELIVVISPKDRTLDAATM
                     TEAYVDEGVLVNSGPFNGMENTKVLEGIADYLEAEGKGRRTIQYRLRDWGISRQRYWG
                     APIPMIDCAKCGIVPVPEKDLPVLLPENVVFSPDGGSPLAALPDFVNTACPKCGGPAM
                     RETDTMDTFVESSWYFDRYCSPGCDVKPGLDRKALDYWMPVDQYIGGIEHAILHLLYA
                     RFYTKVLRDFGVIGVDEPFTNLLTQGMVCKETTKCPEHGYLFPDQAKEGRCAICGKDV
                     IIGKTEKMSKSLKNVIDPDFLVKTYGADTARIFCLFASPPEKDLEWSEQGVEGSFRFL
                     SRLWRIFDDYYEDIQTVSPAAGTLELAGDLKALRRKTHQTIRKVSQDIEDRFHFNTAI
                     SAVMELINVLYGVKHPAKDDKTALSVVREALETAILLLAPIVPHITEELWQLLGKDRA
                     AVDMPWPDYDPACATEEEMTVVIQINGKLRSRMTVPVDCGAEKIKADALADEKIASLI
                     GGNKVLKVIYVPKKLVNLVVAS"
BASE COUNT         1063 a         1444 c         1647 g         1248 t
ORIGIN      
        1 gtaggtgaaa gtttatattg tcttttgctt cggcctttag tcttccgtct aaataactgt
       61 cttatgtttt ctcgaagtga tggtaatcct tgggctgttc gaaatttccg ccccagtgcc
      121 agccacgccg gagaaattcc tggacaatgg ggtgtgaggc aaaaaaggcg cccctgtctt
      181 tggggcgata gacggcgcct tcgggtgcga tcaggcccgt ggggtatatc accggattct
      241 ggaaagggtt gatgtccacc gcgcggccga aagaatgcag agacagtttg ttggtgtcgg
      301 cgatgacgcg gaagttgaag gaagaagaat tgttgtccgc catcgaggct tcgtcgtccc
      361 acttgtacgc gacaatgggg ataactttgc cgatgggtaa tttcagcttc tcgatcagta
      421 gaaagatttc ataaacgtcg tcttcgaggc gcccgtccac gaggatctgg ccttggtggc
      481 gttgtccgtc aaaggaataa tacaggacgt cgatcatgga cagggaatcg atcacttcca
      541 tgggcgccgt agtgccttca atagcttctg caaagctcat ggcgctgtcg atgatgactt
      601 ggttttggtc gttcataaaa aatccttgtt ttgtgcaaac ggccgcggcc acacatgcgg
      661 gcggcaggac cattccctac aataatgctt cgatgattgg attcagggta tctccctcca
      721 gcgagacttc ccggtaaatg ctccggatgg cattgccggt gagccgctcg aaatcggcca
      781 gccggggcgt aatgacgacg ccggccatgt caatggcgcc gggcgagacg aagatgccgc
      841 ttttccccgg tgcaaaatag gcgtcgggcc ggtgtttccg cctcaaaaac acggtgatcg
      901 tccaaccgtg ctttccgtaa gtgcaaaaaa cattcagcag aggctcttcc tgcgtcgcaa
      961 gctgtttccg ggcggcatcc aggaatcgcg tcatgtgctc tttcaggagt ccggcatggt
     1021 ccgacttgaa gagcaggacg cggcgatcgc agttttttgc cggacgatgc aggatggcgg
     1081 atggcgcaat cgggaaagat ccttctttca gttcattcaa aaatgggagc gcacgggccg
     1141 gaatcatctg aaagtgcagg tggtccggcg cggaagcccc gcaggccggg ccgttgtaga
     1201 atacggcata gtcccgcgct gcgtcatggg cgatatccag aaatgcgtcc agtgataacc
     1261 ggatatcctg cggcaggtgc gtgagcgccg ccacggtgag gtggtgttcg aagatgggcg
     1321 ccgggttgca gaggatctga taatcgccgc ggtacagaat gccggactgc tcttccggac
     1381 ggttttcggc gcagagaaaa cagggccgtt tccggatcga ttccgcgtcc acggccgcgc
     1441 cgctgctgac ggtgcgggcc gggttgtact gcagggtgac gctgtagtcg cccaaggaga
     1501 gttcccgggt ttgcaccgac gccagatcac ggcaggccgc cgcgaaggca ggccagcttt
     1561 tcttttgcga ctcaacgagc gcgcggcaca aggcgtgcag ggtttggctt ccgccttcag
     1621 gaaattcagc aaagatttga ttcggtgcaa gtgaaacaga ggaaccagtc tgtcggtctg
     1681 ctgtttgagg aggcgccgca actgcttcat ttgtcattcc gggcaagcga agcgcgaccc
     1741 ggaatccagt atttaaaaat tttggattcc cgcctgcgcg ggaatgacaa cactcttttt
     1801 gatttttatc gtttatattc tcagtagatt tttctttttc gtttatctgc tgcctggctt
     1861 tgatttccag cgttcgcagc cggtccttgt aaaaatcatt gcggttttgc ttttccacgg
     1921 acaggcctgc gtcggtgttg tccttccagc ggcggcagag atagacgctt tcataaaccc
     1981 ggccgatgcg gtactcgcgg gagagcctca gcgccgcggc gtagtcttcc ccgtaactca
     2041 cattgggaaa gccgatccgg cgaagaatgg ccgtgtcgaa agcccgcggg gcgccgatgc
     2101 cgtccactcg caaaagattg ttgtggccgt ttcgcgcggt ccactcccga tgatcgatga
     2161 gacccggcgg aatctttttc aaatgttcgt ttaccagtgt ataggagccc acagccatcg
     2221 cgcagggacc gcggcgcagt tcgttgacca tgatttgcag cgtgtgcggc gacgcgtaaa
     2281 gatcgtcgga atccagttga acggcgtagc ggccgcacag gggcgagtaa atcgcctcgt
     2341 tccagcatcc gccgatgccc agatcatgcc gggacggaac aagatgatga acatgagggt
     2401 attgagcggc aaaggttttc agaagcttcg tcgtgccgtc cgtggagtgg ttgtccacca
     2461 ccagaatatt gaagggaaaa tccgtttgtt gcgcaagcgc gctttgaagg gcgtcggcta
     2521 tggttttccg gcgattgaat acgggaatga caatgctggc cttccagaga atctcttcct
     2581 tctgccggtc cgcggcgggc cttggaggca gccaggcgcc caggcgccgc agatggcggg
     2641 tggctatttt ttccagttgt ttttgccgga tcaaattctc ccgggcgacg taggaaaaat
     2701 gcgcttcgga tttcctgccg gtttttttgg aaggcatttt ttcttttgcg gataccgtgt
     2761 agaggaattc gggcacgcgg atcagttcat gctccagcga aattctcagg cgcaaatcgt
     2821 agaaggcgct gtccggatcg gccggcagcg cgccgtattt tcgaaccgct gaagagacgg
     2881 cggcgcccga taaaatgaaa aaggggccga aatgaaaatg atcgcgcagg gatccggcct
     2941 ggtagtcgtt caacggccgc ccggcgaaac ggctgccgtt tttctcgtag tagtccgaat
     3001 aaatcatgcc ggcccgcgtg tcgcgggcaa gggccagaag tcttcgaagc tccgcagggc
     3061 cgcaggacac gcttttttcc gcatccagcg ccagcagata atccgacgga aggtttttga
     3121 aaaatacgga aggccgtttc agcggccgga agggaccgcc tgcgaaaggc cattgctctt
     3181 ccgcctgcgt ttcatccgcg gcgatccaga gcaaaagccg tccccattga tacactgaac
     3241 ttttcatgat ttcccggacg ccgtcagaag tcggacatca tcagattaaa ggtgttttcc
     3301 gccgtgtcct tcgccagctt ggccagggcc tgttgccggg ccggtttcgg attgacgctg
     3361 tcctgcagtt tgtagtccga cgtgcctgcg acattttgcg aagaccagat ggttttgccg
     3421 ctgtcctttt cgcgaaggct ggcctccagg gtaatcgtca ttctctcttc cgcggccaga
     3481 atgttgttgc ggtaagaaag aacacccgtg gtgatgctga tgacggtgcc gttgatgacg
     3541 gcgtccgcct gttcaagggt aagcgcgctt ttgaaacggc tgctctgaag gatctggtcg
     3601 atgaaggccg cccgcagaat gttttccgct tcggcctccg cggttttgtt gacgaaaggc
     3661 gcgacgtaaa tgttgacgat tcgtttgtcg atgtgttcgc cctgcggggc aaacccgtag
     3721 ccgcagccta tcaggcagaa gcaggacagg cacaaaaacc ctattcggat cgcggacaac
     3781 actcctggtt ttttcatggc gtttccttta agaagcgacg acgaggttga ccagcttttt
     3841 ggggacgtaa atcactttca gcactttatt ccccccgatg agcgaggcga ttttttcatc
     3901 cgccagcgcg tcggccttga ttttttccgc gccgcaatcg accggcaccg tcatccggct
     3961 gcggagcttg ccgttgatct ggatgacgac ggtcatttct tcctcggtgg cgcaggccgg
     4021 atcatagtcg ggccagggca tatcgactgc ggccctgtcc ttgcccagga gctgccagag
     4081 ttcttccgtg atgtgcggga cgatgggcgc cagaagcagg atggccgtct ccagcgcctc
     4141 gcggacgacg gacagggcgg ttttgtcgtc cttagccgga tgtttgacgc catagaggac
     4201 attgatcagt tccataaccg cgctgatcgc cgtgttgaag tggaagcggt cctcaatgtc
     4261 ctgtgagacc tttcggatgg tctggtgcgt ttttcgcctg agcgccttga ggtcgcccgc
     4321 cagctcaagc gttcctgcgg cgggcgagac ggtttggatg tcttcgtaat aatcgtcaaa
     4381 gatgcgccac agacggctca aaaagcggaa ggagccttcc acgccctgct cgctccattc
     4441 caggtctttt tccggcggcg aggcaaacag gcagaagatg cgggccgtgt ccgcgccgta
     4501 ggttttcacc aggaaatccg ggtcgatgac gtttttgagc gacttggaca ttttttccgt
     4561 cttgccgatg atgacgtcct tgccgcagat ggcgcacctg ccttcctttg cctggtccgg
     4621 aaagaggtag ccgtgctccg ggcattttgt tgtttccttg cagaccatgc cctgggtaag
     4681 cagattggtg aagggctcgt ccacgcctat cacgccgaag tcgcgcagca ccttggtgta
     4741 aaagcgagca tagagcagat gcagaatggc gtgctcgatg ccgccgatgt actggtccac
     4801 cggcatccag tagtccagcg ccttgcggtc cagaccgggt tttacgtcgc agccgggcga
     4861 gcagtagcgg tcgaagtacc aggacgactc gacgaacgtg tccatggtgt cggtttcgcg
     4921 catggcgggg ccgccgcatt tggggcaggc ggtgttcacg aaatccggaa gcgcggcgag
     4981 cggcgagccg ccatcggggc tgaagacgac gttttcgggc aggagaaccg gcagatcttt
     5041 ttccggcacc ggcacgatgc cgcattttgc gcagtcgatc atgggaatcg gcgcgcccca
     5101 gtagcgctga cgggaaatgc cccagtcgcg caggcggtac tgaatcgtgc gcctgccctt
     5161 gccttcggct tccagataat cggcgatgcc ttccaggact ttcgtgtttt ccatgccgtt
     5221 gaaggggccg gagttgacca gcacgccttc atcgacgtag gcttcggtca tggtcgccgc
     5281 atcaagcgtc cggtctttgg gagaaataac gacgatcagc tccagattga attttttggc
     5341 gaattcaaag tcgcgctggt cgtgcgtggg cacggccatg acgcagccgg tgccgtagtc
     5401 gg
//
LOCUS       DAOPAF010000087         5384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_13008_length_5384_cov_40.814036, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000087 DAOPAF010000000
VERSION     DAOPAF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13008_length_5384_cov_40.814036"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLQ77_07390"
     CDS             complement(<1..496)
                     /locus_tag="PLQ77_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL10537.1"
                     /translation="MKNQSHAKMIIVVGSLLGLLVVVLFSQLLWAAPKLEMSKSGVKG
                     GDCSACHVQKKMLPEKHADTKAMNWEACKACHNKDNLSLTGKLPNSHHHMLADVNCVK
                     CHGKTKKPEALTMEQCVACHGSTTKLAEKTKDVKPTNPHTSPHYGTELDCNLCHKQHA
                     KSENY"
     gene            complement(575..2131)
                     /locus_tag="PLQ77_07395"
     CDS             complement(575..2131)
                     /locus_tag="PLQ77_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPL10538.1"
                     /translation="MKKDEPTEKTLTRRNFLKITGGSAAVAAAVAGGVTLLPSPADAA
                     CDALPKKWDETYDVIVIGSGFAGLAAAIEAKNGGAKDVVVLEKMPVHGGNSIINGGDF
                     CAAGTKMQKENGVEDSPELLLKDMLRAGAYLNHPDLAKTVAYNSKEALEWTESYLGCK
                     YAKLNFHGGHSVKRAHGTINQSGSEVVNKEMAKAKEIGVKIMMRTKLVKFLSNKEGRI
                     VGLEVRKGYKFPDDNSGKIVFMKAKRAVVLSSGGFSYDVALRQIHDPRLTDKLGSTNH
                     LGATGEALLAACARGAMDVQMDWIQLGPWTSPDEKGFGYTPLFCEPLVGYGLMVNPKT
                     GKRFFKETGNRKERADAILLLGNPALIIGDSYAVKKQVVPHALKGGMEIGAIKKFDTL
                     NDLAKAYDMPVDVFTTQVTRWNSFVEKRKDDDLDCMIFKDAKPTVEAPFYAARLWPKV
                     HHTMGGLVINQDAQVMGFDFKPVKGLYAAGEVTGGVHGAVRLGSVAMADCIVFGRIAG
                     KKAAGEKAWG"
     gene            complement(2367..3902)
                     /locus_tag="PLQ77_07400"
     CDS             complement(2367..3902)
                     /locus_tag="PLQ77_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPL10539.1"
                     /translation="MINESQFFREVSIRICGSLEIDKALVSCFEYVSRVMPVDELIIT
                     IYDAEFKCLKNVAIADAFGAHLSSEEIPLTPLQPHEIQNTERFPRVRKLDNALHDPII
                     RRLAENRKWPPASVLVNRLMIEGKYVGSFLAMVKGEARYTEEDLNVWAQVNETAAIAL
                     ANHLQYREVVRVKDLLADDKRYFQNELRKGAGQTMVGADFGLKDVMEKVRRVAPLLSP
                     VLLLGETGTGKEAIANAIHELSPRNSGPLIKVNCGAIPETLIDSELFGHEKGAFTGAI
                     AQKRGRFERADGGTIFLDEVSELPPQVQIRLLRVLQEKEIERVGGQETIQVNVRILSA
                     TNRDLQRLVEKGLFREDLYFRLNIFPIALPPLRERKNDIPSLVQYFIQKKAKEMALSG
                     IPVFAPGEVNRMMQYDWPGNVRELENMVERAIILARGDCLKFDCAAGENEPKSRESSK
                     QVEETKTLDEVDALHIEAVLSGAGGRVSGKGGAAELLGVNPNTLRHRMRKLGVSFGRA
                     RRH"
     gene            4080..4370
                     /locus_tag="PLQ77_07405"
     CDS             4080..4370
                     /locus_tag="PLQ77_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10540.1"
                     /translation="MEEIRLRTQPKREKEVTDALLDIIESVRENPTLESALVYSHHAK
                     PGGFSLILDWKTMSVPTHGSDTAMLILEGLKPLGLLDHTVMVEKGSFKKHNH"
     gene            4394..>5384
                     /locus_tag="PLQ77_07410"
     CDS             4394..>5384
                     /locus_tag="PLQ77_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL10541.1"
                     /translation="MEKEKKGASLTRRNFLKTAGGAAAVAGLTAGGLTAAPWTAEAAA
                     IPKKWDETYDVVVIGSGFAGLAAAYEAKKAGASVVILEKMRTPGGNSIINGGVVSAAG
                     SPKEQEQGIQDSPDLLLKDMLTAGLYLNHVELAKMVAEQSWPTVKWTIEELGVKYKDK
                     LSQEGGHSVPRMYSTYNQSGSAIVQQQLAKLKELGVQPKMQSYLTQIYRDADGRVKGV
                     QIREKYVFPNADSGKLKNIKARKAVVLASGGFGNDVAFRTIQDPRLTAEFPSTNQPGA
                     TAEALREALRIGCTPVQLSWIQLGPWGSPDEKGMGLSPFFAQLCAAMYGLWIDT"
BASE COUNT         1257 a         1510 c         1324 g         1293 t
ORIGIN      
        1 agtagttttc cgatttggca tgctgcttgt ggcacaggtt gcagtccagc tccgtcccat
       61 aatgaggcga tgtgtgcgga ttggtgggtt tcacgtcttt cgttttttca gccagttttg
      121 tcgtggaacc gtgacaggca acacattgct ccatggtcag ggcttcaggc tttttggtct
      181 tcccgtggca ttttacgcaa ttaacatccg ccagcatatg atgatggctg ttgggcagtt
      241 ttcccgtcaa gctgagatta tccttgttgt gacaggcctt gcaggcttcc cagttcatcg
      301 ctttggtgtc ggcatgtttt tccggcagca ttttcttttg cacgtggcag gcggagcaat
      361 ctccgccttt gacgcccgat ttggacattt ccagtttagg cgcggcccag agcaactggc
      421 tgaacaatac aacaaccagg agaccgagca agctcccaac gacgatgatc attttcgcat
      481 gtgactggtt tttcatattc ctcctaaatc tatattttcc aacggcggac gggcggaacg
      541 cgcaaccctc attcccggcc cgtccggaga tcaattaacc ccaggccttt tcgcctgctg
      601 ctttttttcc ggcgatccgt ccgaacacaa tgcaatccgc catggcaacg ctacccagac
      661 gcacggcgcc gtgtaccccg ccggtcactt caccggcggc ataaagcccc ttgacgggtt
      721 tgaagtcaaa acccatgacc tgggcatcct gattgatgac tagtccgccc atcgtgtgat
      781 gaactttcgg ccacagacga gccgcataaa acggcgcttc caccgtcggc ttggcatcct
      841 tgaagatcat gcaatccaaa tcgtcatcct tcctcttttc cacaaaggaa ttccaacgcg
      901 tcacctgtgt tgtaaagaca tccacgggca tgtcataggc cttggccaga tcattcaacg
      961 tatcgaattt cttgatcgct ccgatttcca tacccccttt aagcgcgtga ggaacaacct
     1021 gcttcttcac ggcataggaa tcaccgatga tgagggcggg gttccccaaa agaaggatgg
     1081 catcggcgcg ctccttgcgg ttgcctgtct ctttaaaaaa acgttttccg gttttgggat
     1141 taaccatgag accatagcca accagcggtt cgcagaaaag cggcgtatag ccgaatccct
     1201 tttcatccgg gctggtccac ggcccgagtt ggatccagtc catctgaaca tccatcgcgc
     1261 ctcttgcgca ggccgccagc aaagcttcac cggttgcacc gagatgattg gtggaaccca
     1321 gcttgtctgt tagccgggga tcatgaatct gccgaagagc gacatcatag gaaaatccac
     1381 cggagctcag aacgacggcc ctttttgcct tcatgaaaac gatttttccc gagttatcgt
     1441 ccggaaattt atatcccttg cgaacttcca gtccgacaat gcgcccttct ttattggaca
     1501 ggaactttac aagcttggtg cgcatcatga tttttacgcc gatctctttg gcttttgcca
     1561 tctccttatt cacaacttcc gatccggact gattgatcgt cccatgcgct cttttgacag
     1621 aatggccgcc gtgaaaatta agttttgcat acttgcatcc caggtaggac tccgtccact
     1681 ccagggcttc ctttgaattg tatgcaaccg tcttggccag atcggggtga ttcagatacg
     1741 cgccggctct cagcatgtcc tttaaaagga gttccggaga atcttccacc ccgttttcct
     1801 tttgcatctt cgtcccggca gcgcagaaat cgccgccgtt gatgatcgaa ttgccgccgt
     1861 gcaccggcat tttttcaagc accacaacgt cttttgctcc gccgttcttc gcttcaatgg
     1921 cggccgccag tcctgcaaac ccgctgccga tgacaatcac gtcatacgtt tcatcccatt
     1981 tcttcggcag agcatcgcag gccgcatccg ccggggaagg aagcagcgtc acaccgccgg
     2041 ccacggccgc ggctacggcc gccgaaccac ctgtaatttt taaaaaattt ctccgggtta
     2101 acgttttttc cgtaggttcg tccttcttca tggattttcc tcctgttggt tatgatcgtc
     2161 cctgatcgaa tttataaact ctgggaaaga acagaaaaaa tatagtggtt attgcggttt
     2221 catgaattga aagttactct atcgacgcga caatttcgaa aaatgatgca cgaattcagt
     2281 ctgttttctt atacatcttt ggtaatttgt ctgtcaagcg cgcaaatggg aagggcgaaa
     2341 agccggagat ttcattggtg atgaggtcaa tggcgccgtg cgcggccgaa cgaaacaccc
     2401 agctttctca tgcggtgcct cagggtattc ggattcacgc ccagcaattc cgcagcaccc
     2461 cctttgccgc ttacacgccc cccggccccg gacaaaacag cctcaatgtg gagggcgtcc
     2521 acctcgtcca gcgttttggt ttcctccacc tgtttcgacg attcccgact tttaggctca
     2581 ttctcgcccg ccgcacagtc aaatttcagg cagtcgcccc gggcaagaat aatagctctt
     2641 tccaccatat tctccagttc gcgaacattc cccggccagt catactgcat catccggttg
     2701 acctcacccg gagcaaaaac cggaatgccg gacagagcca tttccttggc tttcttctgg
     2761 ataaaatact gaacgagaga cgggatgtca ttttttcttt cgcgcaacgg cggcagggca
     2821 atggggaaaa tattcagccg gaaataaaga tcctcgcgaa acagtccttt ttccacaagg
     2881 cgctggagat cccggttggt cgccgataga atccgcacgt tgacttgaat ggtttcctgg
     2941 cctcccaccc gttcaatttc tttttcctga agcacacgca gcagacgaat ctgcacctgc
     3001 ggcggaagct ccgaaacttc atcgagaaaa atggtccccc cgtccgcccg ctcaaaacgt
     3061 ccccgtttct gcgcgatcgc cccggtgaaa gcgccttttt catgcccgaa aagctcgctg
     3121 tcgatgagcg tttccggaat cgccccgcag ttgactttaa tgagcggtcc gctgtttctt
     3181 ggagaaagtt cgtgaatggc gttggcaatg gcttccttcc cggtgcccgt ctctcccaga
     3241 agcagaacgg ggctcagcaa gggggcgacg cggcggacct tttccatcac gtccttcaaa
     3301 ccgaaatccg cgcccaccat cgtctgtccc gcgcccttgc gaagctcgtt ttgaaaatag
     3361 cgtttgtcat ccgccagcag atccttgact ctgaccacct cgcgatactg cagatggttg
     3421 gccaaggcaa tcgccgccgt ctcattcacc tgagcccaaa catttaaatc ttcctccgtg
     3481 tatcgcgcct ctcctttcac catggccaga aaagatccca catatttgcc ttctatcatc
     3541 agccggttca ccagcacgga cgcgggaggc catttacggt tttcggccag acgtcggatg
     3601 atcggatcgt gcaaagcatt atccagtttc ctgacacgag gaaaacgttc ggtattctga
     3661 atttcatggg gttgaagggg cgtcagggga atttcctccg aagacagatg cgccccaaaa
     3721 gcatcggcaa tggccacatt tttcaagcat ttgaattccg cgtcgtatat cgtgatgatt
     3781 aactcatcca ccggcatgac ccgactcaca tattcaaagc aggacaccaa agccttatcg
     3841 atttcaaggc ttccgcaaat tcggatggaa acttcccgaa aaaattgact ttcattaatc
     3901 atgatttctc gtccgtccaa gcggttatca tatgtgacaa tttaatacca atttaccata
     3961 taagacaatt actttttatc acatgcggta aatttatgaa tggacgtatt tcatattatt
     4021 gaaataataa agcattttaa ttggcacgct gaatgctaat caggaaagac atgagatgga
     4081 tggaagaaat acggttgaga actcagccca aaagagaaaa ggaggttaca gatgcgcttc
     4141 tggatatcat tgagtccgtt cgggaaaatc caaccctgga atcagctctg gtttattcac
     4201 accatgccaa gcccggcggg ttcagtctga ttctggactg gaaaacaatg tccgtcccga
     4261 cccatggaag cgacaccgcc atgctgattc tcgaagggct caaaccgctt ggcctgcttg
     4321 atcataccgt catggtcgaa aaaggaagtt ttaaaaagca taaccactaa caagaaagac
     4381 aggaggctgg aagatggaaa aagaaaaaaa aggagcatcc cttaccagac ggaatttttt
     4441 aaaaacggcg ggtggtgcgg ccgccgtcgc cggtctgacc gccggcggac tgaccgctgc
     4501 gccatggacc gccgaggcgg cggccattcc gaaaaaatgg gatgaaacgt acgacgtcgt
     4561 tgtgattgga agcggttttg ccggactggc ggccgcatac gaagccaaga aggcgggagc
     4621 atccgtcgtc attctggaaa aaatgcgcac gccgggcgga aactccatta tcaacggcgg
     4681 cgttgtgtcg gcggcgggct cccccaaaga acaagagcag ggcattcagg attcgcccga
     4741 cctgctgctc aaagacatgc tgaccgctgg cttgtacctc aaccatgtcg agctggctaa
     4801 aatggtggcc gaacagtcgt ggcctaccgt caagtggacc atcgaagaac tgggcgtcaa
     4861 gtataaagac aaactctctc aggaaggcgg ccattcggtg ccccggatgt acagcaccta
     4921 caaccagagc ggctcggcca tcgtgcaaca gcagctggcc aaactcaagg agctgggtgt
     4981 tcagccgaaa atgcaaagct atctgacgca aatctaccgc gacgcggacg gccgggtcaa
     5041 aggcgtacag atccgtgaaa aatatgtttt ccccaacgcg gacagtggga aactcaaaaa
     5101 catcaaagcg cgcaaggcgg tcgtcctggc cagcggcggt ttcggcaatg acgtagcgtt
     5161 cagaaccatc caggatcccc gcctgacggc ggaattcccc agcaccaatc agcccggagc
     5221 gactgctgag gcccttcggg aagccctgcg tatcggttgc acgcccgtcc agttgtcctg
     5281 gattcaactt ggcccgtggg gcagtccgga tgaaaaaggc atggggttgt cgcctttctt
     5341 tgcgcagttg tgcgcggcca tgtacggcct ctggatcgac acga
//
LOCUS       DAOPAF010000088         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_13535_length_5205_cov_41.908350, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000088 DAOPAF010000000
VERSION     DAOPAF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13535_length_5205_cov_41.908350"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..881)
                     /locus_tag="PLQ77_07415"
     CDS             complement(216..881)
                     /locus_tag="PLQ77_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10542.1"
                     /translation="MQKENYRNKTTIMLFPTIDQTSTVSSDIISMKNWRHADIHIAVG
                     PIGKAAAVTLEKSAAVAAASTAMSFSKYLSTGFRLKYDGASVDTPAAAGETVTGAGGG
                     IGYVYEDLGGKLVCYAHNGTTFVDNEVLTFSGGKTAVADGIQYDEDILVPRTASSDTF
                     NLAAVANKEYVIPVDASDLGDGYDCVQVEIADCDTATHLCIWADLSDPRYAAEIPETA
                     IYD"
     gene            complement(937..2310)
                     /locus_tag="PLQ77_07420"
     CDS             complement(937..2310)
                     /locus_tag="PLQ77_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPL10543.1"
                     /translation="MNQYQKRMDAAFKKMEDIRKKAESEGRALTPEELEARAALKAEI
                     DQAKREWDDFKAEEELRAELYGNGGGGALTVAGDPQIEIPDQPIYRGSNATALGAQLL
                     DIRTLSAPSNFEDVEIRAARGRLEKSQKRYSEKMEALARKENRAAATGGFTVGVPSDG
                     GFFLQGETVVDLMTNGFNNSEVLPRTARRTLTATQFVEIFGIDESSRVTGSRGGGIRV
                     YTNKELGEFTASKTKFKMIRVEPQKLTGLFYASGEWMRNVTFLGQEVRGLFGEEFAFK
                     CQDLAIRGSGAGEALGILNAGCLVSVAKEANQVAKTILTANLSKMWSRFRGRNPVWFI
                     NRDCGPQLDVLSITAGTAALEPRFVNYGADGILRIKGAPVVEIEQCETLGTVGDIILA
                     DWGQYVSADQGSIDEAMSIHVNFIYDQNTYRFIYYFDGQPRWASPITPFKGSNTVSPF
                     VVLATRS"
     gene            complement(2323..2943)
                     /locus_tag="PLQ77_07425"
     CDS             complement(2323..2943)
                     /locus_tag="PLQ77_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HPL10544.1"
                     /translation="MKEKRKTIEVRSIEADGGGLKKIVGYAAVFDKPSEDMGFIEYVR
                     KGAFKKALARSDARALFNHDTDTLPLGRQSAGTLLLREDDTGLYYEINPPDTQSARDL
                     MTAIDRGDIREASYGFTVDVDEWDYSDPDVIKRTIIEVREVYDISPVVFAAFNDTTVA
                     LRKLEENRKNAPPGDGEAGGGSEGSAAANTVLLEEEDNLYKKIMGF"
     gene            complement(3016..4275)
                     /locus_tag="PLQ77_07430"
     CDS             complement(3016..4275)
                     /locus_tag="PLQ77_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPL10545.1"
                     /translation="MSIIGGMERRMAAGTAGLADSWYYPGGFFYGGTGGFKTKSGASI
                     SEMNAMQLSIVWCCIKILAEDSASLPLHLYKRRKSGGKDRAWDDPRYIMLHDRPNPEM
                     TAMSFRESFMSHLLAWGNAYAEIERTRGALKIPVALWPITPNRVKVKRDDRKRLIYEI
                     SMAGTGLQDVTLQRGNVLHTPGLSYNGLVGYSPVAAAREAMGLGKTLEEYGCAFFQNG
                     IHPSFIISSKVPIKDTKMRREALEEVHAGLGNAHRVMFIEEAEKVERLGIPNDEAQFL
                     DSRKFTNIDIGTRIYRLPPQMYGEYDKASTYASAEQFAIDYVTKSLRAWLVRLEQSYN
                     MSLLDPSEYGEYFFEHNIEGLLRGDIQSRYQAYMMGKRNGWLNADEIRELENLNPIPD
                     GMGQEYIVEKNMIGLGDLGADLTGKAA"
     gene            complement(4608..4784)
                     /locus_tag="PLQ77_07435"
     CDS             complement(4608..4784)
                     /locus_tag="PLQ77_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10546.1"
                     /translation="MTEILTTWKEIAVYLRVSVKTAKRYRKNKGLPVQRDPAGHPTLH
                     KQSADEWKLKAKTD"
     gene            complement(4793..>5205)
                     /locus_tag="PLQ77_07440"
     CDS             complement(4793..>5205)
                     /locus_tag="PLQ77_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HPL10547.1"
                     /translation="LDRIGVDQSGIGAIIDELEGGDESGDLAIANDRIVGIPQGWRLN
                     SAIKTTERKVAAKEIIHSGSRMMAWCVGNARVEPKGNAILITKQASGTGKIDPLMAVF
                     NAVALMAMNPEARQVKSVYEGLNKEQTIARITGR"
BASE COUNT         1006 a         1539 c         1431 g         1229 t
ORIGIN      
        1 agccggttcg ccgcctccag ggacataatc tcgttgccgg acgcatcata atagacaaga
       61 tcgccgtcaa cgccctttgc ggtgatgttc aacagttccg cccccgcgaa gatggcgggg
      121 atgagcaacc cgacggcaag gacggcgatt aaaagtttct ttttcatttc atcctccttg
      181 tgaggggaag ggccggagcc ctccccatcg ggttgttagt catagatggc cgtttcgggg
      241 atctccgcag catagcgagg atcgctaaga tccgcccaaa tgcaaagatg agtcgccgta
      301 tcgcaatcgg caatttcaac ctgcacgcaa tcataaccat cgccgaggtc agaggcatca
      361 acggggatga catattcttt attggcaaca gccgcgaggt tgaatgtatc gctggaagcc
      421 gtccgcggga ccagaatatc ctcatcgtac tgtatgccgt ctgcaacagc cgtctttccg
      481 ccggagaagg tcaggacttc gttgtccacg aaggtcgtgc cgttatgggc atagcagacc
      541 agcttaccgc cgaggtcctc gtaaacatat ccgatgccgc cgccagcgcc ggtgacggtc
      601 tcacccgccg ctgcgggagt atccacggaa gcgccgtcat atttgaggcg gaaaccggtg
      661 ctcagatatt ttgagaatga catggccgta gatgccgccg cgaccgccgc acttttctca
      721 agagtaaccg ccgccgcctt cccgatagga ccgacggcga tatgaatatc agcgtgacgc
      781 cagttcttca tgctgataat atccgaggat accgtgcttg tctggtcaat cgtcgggaaa
      841 agcattatgg tggttttgtt tctgtaattc tctttctgca ttgctctttc ctccttatga
      901 gaggccgggg attgcgcccc ggcatgtcgt tattggttag ctccgggtcg ccagcacaac
      961 gaacggcgaa acggtgttgc tgcccttgaa cggcgttatc ggcgacgccc accggggttg
     1021 tccgtcaaag tagtagatga agcggtaagt attctgatcg taaatgaaat tgacgtggat
     1081 actcatggcc tcatcaatac ttccctggtc ggcgctcaca tactggcccc aatcagcaag
     1141 gatgatgtcg ccgacggtgc ccagggtttc gcactgttcg atttcaacaa ccggagcgcc
     1201 cttgatgcga aggatgccgt ccgcgccgta gttgacgaaa cggggctcca gggctgccgt
     1261 gcccgccgtg atggagagaa cgtcaagctg cgggccgcaa tcccggttga tgaaccagac
     1321 cggattccgt ccgcggaaac gcgaccacat ttttgagagg ttggccgtca ggatggtttt
     1381 tgccacctgg tttgcctctt tcgccaccga gacgagacat ccggcgttca ggatacccag
     1441 ggcctccccg gcgccgctgc cccggattgc caaatcctgg cacttgaagg cgaactcttc
     1501 accgaacagg ccgcgcactt cctgccccag gaaggtgacg tttctcatcc actcgccgga
     1561 agcgtagaaa agcccggtca gcttctgagg ctcgacccgg atcatcttga actttgtctt
     1621 gctggccgtg aactcgccga gttccttgtt ggtatagacc cggatgccgc cgcccctgct
     1681 gccggtcacg cgggaacttt cgtcaatgcc gaagatttcc acaaactgcg tcgccgtcag
     1741 ggtccgtctt gccgtccggg ggaggacttc gctgttgttg aagccgttgg tcatcaggtc
     1801 aacaaccgtt tcaccctgga ggaagaaacc gccgtcggac ggaacgccca cggtaaagcc
     1861 gccggtcgcc gccgcccggt tttccttcct ggcaagggct tccatctttt cggaatagcg
     1921 cttctggctt ttctcaagac gcccgcgggc tgcccggatc tccacgtcct cgaaattcga
     1981 cggcgcggaa agggtccgaa tgtccaggag ctgcgccccc agggccgtcg cattggagcc
     2041 gcgatagatg ggctgatcgg ggatttcaat ctgaggatcg ccggccaccg tcaaagcgcc
     2101 accgccgcca ttcccgtaca actccgcccg cagttcttcc tcggctttga aatcatccca
     2161 ttcgcgtttg gcctggtcaa tctccgcctt gagggccgcc cgagcctcaa gctcctccgg
     2221 ggtcagcgcc cgcccctcac tttccgcctt tttgcggatg tcctccatct tcttgaaggc
     2281 ggcatccatc cttttctgat actgattcat gtcgattcct ccttaaaaac ccatgatttt
     2341 tttataaaga ttgtcctctt cttcaaggag gaccgtgttt gctgctgccg agccctccga
     2401 cccgccgccc gcttcaccat ctccaggagg ggcgtttttc ctgttttctt ctaattttcg
     2461 gagcgcaacc gtcgtgtcgt tgaaggcggc aaacacaacc ggggagatgt cgtacacttc
     2521 cctgacctcg atgattgtgc gcttgatcac gtcgggatcg gaataatccc attcgtcaac
     2581 gtccacggtg aaaccatagg acgcctcgcg gatgtcgccc cggtcaatgg cggtcatgag
     2641 gtcccgcgcc gattgcgtat ccgggggatt gatttcgtaa tacaggccgg tgtcatcctc
     2701 ccgcagtagg agcgtccccg cgctctgccg gcccagggga agcgtgtcgg tgtcgtgatt
     2761 gaatagagcg cgggcatcgg agcgggccag ggctttcttg aaggccccct tgcggacata
     2821 ttcaatgaac cccatgtctt cggacggctt atcgaatacc gccgcataac cgactatctt
     2881 tttcaggccg ccgccgtccg cctcgatgga gcggacttca atcgtttttc tcttttcctt
     2941 catcgctgtc acctccgcgc tttgtttcgc gggtctcgta ttttttcggc gggattgcct
     3001 tttcgtaggt gggtttcatg ccgctttccc cgtcaagtcg gcccccaaat cgcccaggcc
     3061 gatcatgttt ttctcgacta tgtattcctg ccccatgcca tcggggatcg ggttcaggtt
     3121 ttccagctcc cggatctcat cagcgttcaa ccagccgttg cgcttcccca tcatgtacgc
     3181 ctgatagcgg ctctggatgt cgccccggag aaggccctcg atgttgtgct caaagaaata
     3241 ttcaccgtat tcggaggggt ccaggaggct catgttgtag gactgttcaa gacgtaccag
     3301 ccaggcccgg agtgatttag tcacatagtc gatggcgaac tgctccgcgc ttgcgtaggt
     3361 gctggccttg tcatattcgc cgtacatttg cgggggaagg cgatagatgc gggtgccgat
     3421 gtcgatgttt gtgaatttcc gcgaatcaag gaactgcgcc tcatcgttcg ggatgccgag
     3481 gcgttcgacc ttctccgcct cttcaatgaa cattacccgg tgagcattgc ccaggccggc
     3541 atggacttcc tccagggctt cccggcgcat tttcgtgtct tttatgggga ccttcgatga
     3601 gattataaac gagggatgga tgccattctg aaagaaggcg cacccgtatt cttccagggt
     3661 cttacccagg cccatcgcct cccgtgccgc ggcgaccggg gaatagccga caaggccgtt
     3721 ataggaaagg ccgggagtat gcaggacgtt accgcgctgc aaagtcacgt cttgcaggcc
     3781 ggttccggcc atgctgatct cgtatatcag ccgctttcgg tcgtcccgtt taaccttgac
     3841 gcggttcggc gtgatcggcc agagggccac agggattttc agggcgccgc gggtccgttc
     3901 gatttccgca taggcattgc cccaggccag caggtgagac atgaatgatt cccggaagga
     3961 catcgccgtc atttcggggt tgggccggtc gtgcagcatg atatagcgcg gatcatccca
     4021 ggcgcggtct ttaccgccgg atttgcgccg cttgtagagg tgaaggggga gagatgccga
     4081 gtcttcggcc aggattttga tgcagcacca aacgattgag agctgcatgg cgttcatttc
     4141 agagatggac gccccggatt tggtcttgaa gccgcctgtt ccgccataga aaaagcctcc
     4201 tggatagtac caggagtcgg ccaggccggc tgtccccgcc gccattcgcc tttccatgcc
     4261 gccgattatg ctcatttagc gggcctcctg atgagccatc cgagccccaa aagcattgaa
     4321 acggccccga aaactgccca tccgagccag ggacggagct gataaagccc atatcccagg
     4381 caaaacaggc cgccgaatac gagaaaatcc cgaatatcgg cgacttttgc ggccttttgg
     4441 gccgaattta cgacaaattc acataaatac cttaatttct tcacatattc gccgtttctg
     4501 caaaataaat tgcattttcc ggcaatattg tggcatattt ataaatttag cgtaatggga
     4561 caaaaggggg acaaaagggg gacaaaaggg ggacaaaagg gggacagtca gtcagttttg
     4621 gcctttaatt tccattcgtc tgctgactgc ttgtgcaagg tggggtgtcc ggcggggtct
     4681 ctttggacgg gaaggccctt gttcttgcgg tatcgcttcg cggttttaac ggaaactctc
     4741 aaataaacgg cgatttcttt ccaagtcgtc aggatttcgg tcattgcgcc tcctatcgcc
     4801 ccgttatccg ggcgatcgtc tgctctttgt tcagcccttc gtaaaccgac ttcacctgcc
     4861 gcgcctcggg attcatcgcc atcagggcca cggcgttgaa caccgccatc aacgggtcaa
     4921 tcttcccggt cccgctggcc tgcttggtga tcaagatcgc gttccccttc ggctctaccc
     4981 tggcattacc gacgcaccag gccatcatcc gggatccgct atggatgatt tcctttgccg
     5041 ccaccttccg ctcggtcgtc ttgatcgcgc tattaagccg ccagccctgg gggatgccga
     5101 caatccggtc attcgctatc gccaggtcgc cgctttcgtc gccgccctca agttcgtcaa
     5161 tgatcgcacc aatgccggat tgatccacgc cgatcctgtc cagga
//
LOCUS       DAOPAF010000089         4775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_14897_length_4775_cov_60.035381, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000089 DAOPAF010000000
VERSION     DAOPAF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14897_length_4775_cov_60.035381"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLQ77_07445"
     CDS             complement(<1..707)
                     /locus_tag="PLQ77_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10548.1"
                     /translation="MLKKILAGAVLFLLMLLAETGFLHAQEIKLSVFNFGTANIEASG
                     LGTSVTNALISNLSRNPSISILDRKDLETFLNLNDLQQNDQLDNVVNIGSRLGLDFIV
                     VGSVAKRGSAITVKCGLVQIDKRKEVYSTQVRAQGESALSAEIAKLASLITSVLTKSS
                     TGPESTSAPGTGGGPGAGAPVNFKGVPGNKKIILRWQPVPGFAAAGYEVYRALNANGP
                     FAMLGQTDKTEFADQNV"
     gene            complement(722..2707)
                     /locus_tag="PLQ77_07450"
     CDS             complement(722..2707)
                     /locus_tag="PLQ77_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPL10549.1"
                     /translation="MKSRKTYMAIASAVVLLVLAACVGTNESLKQGQEFAKQNRWDEA
                     MIYFEKAVSEEPDNSEFKSELTRAKQEVAKARLARAKQSHAAAGQNFMALERLSKEAD
                     EILKLDPNDPEVRSFSATVNGQVNNLKGNLKNLYQQAQADMQKEDWLAAIDKLKQVNA
                     IFPNYEDTGNRLRRSLSEGTRAMYQQGTKLGKQEDWKGAAESFKAVMDVNPKYLDAAQ
                     QYANAVKNDNPAYFAAEAAKAEGAGKMDRAIAMYEKALEYPQADPKLNDSLAALKAKS
                     SQIYLNDSIELFKQERLYGSFKKLDLAGKYAPALRSEPAYAEHANRLCEALMKRANKF
                     AEKELWGNALVWLQLVDRINPEYEGLFQKMNEVKDPISRRVRKSIAVFDFGSPSTDKD
                     AGKIAANRLLAYLHQNASVDLRIIERENLQSILREMQLSQTGLVDIKTAQSVGKMRGI
                     DTFIMGDVLQFSAKYTDVATTNQVRVLINEEDVPNPDWSDWRILNPKPSAEDLKNAPA
                     RTIKKPNYQFLPNKQGTARINAMLEISFKLVDTQSGEVNANTINGKLVKEDKYQEGLN
                     IAGIKADPLELPTEGEVLDQLAKEKIAEMGRDVLKHFQSLEVEYFNKGQDLQKRRNYE
                     AATERYTDAIFDEKLKTISTPISQKSAELIELINEFN"
     gene            complement(2746..3837)
                     /locus_tag="PLQ77_07455"
     CDS             complement(2746..3837)
                     /locus_tag="PLQ77_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10550.1"
                     /translation="MKKWTLFLVMLFLAASVFPVYAQKKITAKGMAANQRNIDIACSR
                     ALDEAQRNAVEQATGVMITSTTNVENFQVKMDRILSESKGFINAYKVLSEKKAGTDCE
                     VVIEADVSTGKLRDRMMAVNLIMARKSKPRVMLVFKDAVAEAVMAKYLLGHNFKLVDA
                     DVSSKNREYDRLKDASEARDIRGLAQHYGAEVIIVGTVSADSKSSTIYNIEMNKNTVV
                     VTGKVINGDTGEIITTASETKSDNNMKGDYKALTEETSSKLISKLVDDVLENWSKELA
                     NTASVKLIVTGLDEETLEDLKAELGEEIKGLKEVNERFYAGGKAEFDLEVEGNAQDLA
                     RDLRKITVNKRKIKIKSRTQNRVEAVLLP"
     gene            complement(3957..4544)
                     /locus_tag="PLQ77_07460"
     CDS             complement(3957..4544)
                     /locus_tag="PLQ77_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10551.1"
                     /translation="MKCKRLWVIAAALGMMLVFPALEKPAFAHEKIVDFILKVHPDGP
                     ASAIPEDFLRTGSDDGYGYAHEGYIDGFKIICRSKDGIVKSVHAQKQFNTGDLLAEHT
                     YYNILGSVLSRLMKKYNGTLVNEKGRGESTDKVPYDYGEYCYYTALKGAGKTIKTRDY
                     KLGFWYDLGVWKRCDNYSYNGENPGNVIIEFRPGN"
BASE COUNT          835 a         1333 c         1266 g         1341 t
ORIGIN      
        1 tccacgtttt gatcggcgaa ttccgtttta tccgtctggc ccagcatggc aaacggaccg
       61 ttcgcattca gggctcgata gacttcatat ccggcggccg cgaagccggg aacgggctgc
      121 cagcgcagga tgattttttt gttgcccggg acgcccttga aattgacggg agcgccggcg
      181 cccggtccgc cgcctgtgcc gggtgcggac gtgctttcag gtcccgtgct gctcttggtc
      241 agcaccgagg taatcaggga ggcaagcttg gcgatttccg cggacagcgc cgattcgccc
      301 tgcgcccgaa cctgggtgct gtacacttct tttctcttgt cgatctggac cagtccgcat
      361 ttgacggtaa tggcggatcc tctcttggcg acggagccga cgacaataaa atcgagcccc
      421 agccgcgagc cgatattgac gacgttgtcc agctgatcgt tttgctgaag gtcgttcagg
      481 ttcagaaacg tctccaggtc tttgcggtcg aggatggaaa tggaggggtt tctgctcaga
      541 ttgctgatca gcgcgttggt gacgctggtt cccagccccg acgcttcaat gttggccgtc
      601 ccgaagttaa agacggacag cttgatttcc tgtgcgtgga ggaatcctgt ttccgcaagg
      661 agcatcagca agaataaaac agcgccggca agaattttct tcagcaagat cggtctccgt
      721 attaattgaa ttcgttgatc agctcaatca gctccgccga tttctgtgaa atcggcgtgg
      781 agatggtttt caatttttca tcgaaaatgg catccgtgta ccgttccgtg gccgcctcgt
      841 aattgcggcg tttctgcagg tcctgcccct tgttgaaata ttccacttcc aggctttgaa
      901 agtgtttcag cacatcgcgt cccatttccg caattttctc tttggccaat tgatccagca
      961 cttcgccttc cgtcggcagc tccagaggat cagccttaat gccggcgata ttcaagcctt
     1021 cctggtattt gtcttctttc accagttttc cgttgatcgt gttggcgttg acttcgcccg
     1081 attgggtatc caccagcttg aaggatattt ccagcatggc gttgattctg gcggtgccct
     1141 gcttattggg caggaactgg tagttcggtt ttttaatggt ccgggcggga gcgtttttaa
     1201 gatcctccgc cgaaggtttg ggatttaata tgcgccaatc cgaccagtca ggattgggga
     1261 cgtcctcttc attgatcagg actctgacct gattcgtcgt cgcaacgtcc gtgtactttg
     1321 cggaaaactg cagcacgtct cccatgatga atgtgtcgat gccgcgcatt ttgccgacgc
     1381 tctgcgccgt tttgatatcc accagccccg tctggctgag ctgcatttcc cggagaatgg
     1441 attgcagatt ttcccgctcg atgatgcgca gatcgacgct ggcgttctga tgcagatagg
     1501 ccaggagccg gttggcggct attttgccgg catctttgtc cgtgctgggg gagccgaaat
     1561 caaaaacggc gatcgacttc cggacgcgcc gcgagatggg atccttcact tcgttcattt
     1621 tctggaacaa cccttcgtac tccgggttga tcctgtcgac aagctgcagc cagaccagcg
     1681 cgttgcccca cagttccttt tccgcaaatt tattggccct tttcatcagg gcttcgcaca
     1741 ggcggtttgc gtgttccgcg taagcgggtt cgctgcgcag ggccggtgca tacttgccgg
     1801 ccagatcgag cttcttaaac gagccgtaaa gccgttcctg tttgaaaagt tcgatggagt
     1861 cattcaggta aatctggctc gattttgctt tcagcgccgc cagactgtcg ttcagtttgg
     1921 ggtcggcctg cggatactcc agggcttttt catacatggc aatggcccgg tccatcttcc
     1981 cggcgccctc ggcttttgcc gcttccgccg cgaagtaggc gggattgtcg tttttgacgg
     2041 cattcgcgta ctgctgggcc gcatccagat atttcggatt gacgtccatg accgccttga
     2101 agctttccgc ggcgcccttc cagtcttcct gttttcccag tttggtgccc tgctggtaca
     2161 tggccctggt gccctcggac agggagcgac ggagccggtt gcccgtatct tcataattcg
     2221 ggaagatggc gttgacctgc ttcagcttgt cgatggccgc cagccagtct tccttctgca
     2281 tgtcggcctg ggcctgctga tacaggtttt tcaggttgcc cttcaggttg ttgacctgtc
     2341 cgttgaccgt tgcggaaaaa gacctgactt ccgggtcgtt cgggtcgagt ttcaggattt
     2401 cgtcggcttc tttggacagc cgctccagcg ccatgaaatt ttgtccggct gccgcgtggg
     2461 actgtttggc cctcgccagt ctggccttgg ctacttcctg ctttgcccgg gtcagttcgc
     2521 ttttgaattc agagttgtcc ggttcttcgc ttaccgcctt ttcaaaataa atcattgctt
     2581 catcccagcg gttttgtttg gcaaactcct gtccctgctt gaggctttcg ttggttccca
     2641 cgcaggctgc caggaccaaa aggaccaccg cggatgcaat cgccatgtag gtttttctac
     2701 ttttcattca atcctcccca ctcaaaagtt tccgaatatc gatccttaag gcaatagaac
     2761 cgcctcaact ctgttttggg ttctgctttt gattttgatt ttacgcttgt tgacggttat
     2821 tttccgaaga tcgcgggcca gatcctgcgc gttgccttcc acttccaggt caaattccgc
     2881 tttgcctccg gcgtagaaac gctcgttgac ttccttgagg cccttgattt cttcccccag
     2941 ttcggccttg agatcttcaa gcgtttcttc gtccagtccc gtgacgatca gcttgaccga
     3001 ggcggtgttg gccagttcct tcgaccagtt ttccaatacg tcatccacaa gctttgaaat
     3061 cagcttggag gaggtttctt ccgttaaggc tttgtagtcc cccttcatgt tgttgtcgga
     3121 tttggtttcg ctggcggtgg tgatgatttc ccccgtgtcc ccgttgatca ccttgccggt
     3181 gaccaccacc gtgttcttgt tcatctcgat gttgtaaatg gtggaggact tgctgtccgc
     3241 gctcaccgtt ccgacgatga tcacctccgc gccgtagtgc tgcgccagcc cgcggatgtc
     3301 tctggcctcc gaagcgtcct tcaggcggtc atactccctg tttttgctgg aaacatccgc
     3361 gtccaccagc ttgaagttgt gcccgagcag atatttggcc atgacggctt ccgccacggc
     3421 gtccttgaac accagcatga cccggggctt ggatttgcgc gccatgatca gattgacggc
     3481 catcatcctg tcgcgaagct ttcccgtgct cacatccgcc tcgatgacca cttcgcagtc
     3541 cgttccggct ttcttttcgg acaggacttt ataggcgttg atgaagccct tggattccga
     3601 aagaatgcgg tccatcttca cctggaaatt ttccacgttg gtggtgctgg taatcatcac
     3661 gcccgtcgcc tgctccacgg cgttgcgctg cgcttcatcc agagcccggc tgcaggcgat
     3721 gtcgatgttt ctctggttgg ccgccattcc tttggcggtg atttttttct gggcgtaaac
     3781 gggaaaaacc gaagccgcca gaaacagcat cacaagaaag agcgtccatt ttttcatgaa
     3841 attcatccct tgtgttcaga aatttttaaa gtaattacgc ttctacatac tgaatttgac
     3901 catgggtgtc aagttgaaag cattggaaag caggccggag atttccggat ccggcgtcag
     3961 tttcccggcc ggaattcaat gatgacgttc cccggatttt ctccgttgta gctgtagttg
     4021 tcgcaccgct tccagacgcc gaggtcatac cagaatccca gcttgtagtc cctggtcttg
     4081 atcgtctttc ccgctccttt cagcgccgta tagtagcagt attccccgta gtcgtacggc
     4141 accttgtcgg tgctttcccc ccgccccttc tcgttgacga gcgtgccgtt gtattttttc
     4201 atcagcctgg acagaacgct ccccaggatg ttgtaatagg tgtgctcggc caagaggtcg
     4261 cccgtgttga actgcttttg cgcgtgcacg gatttcacga tgccgtcttt ggaacggcag
     4321 atgattttaa agccgtcgat atagccttca tgcgcgtagc cgtagccgtc gtcgctgccc
     4381 gttctcagga agtcttcggg aatggccgaa gccggcccgt ccggatgcac tttgaggatg
     4441 aaatcaacga ttttttcatg ggcgaaggcc ggtttttcga gcgccgggaa aacgagcatc
     4501 attcccagcg ccgccgcgat cacccaaagt cttttgcatt tcattgtttt ttcctctttt
     4561 ctccctttcc ggggtgcttc acgaatttgt aaaaccgcca ccttcatatg tcaagacaaa
     4621 acttgcgttc cggcgcacgt gaggcgaccc ggcacagcgc gttcatcagg acgttttgcc
     4681 gtgaagggcg atgaatatct gtatgttttt cgaccgcttt tccgcttgac gggggccggc
     4741 ttttcttcta tggtttcagc ggttacgggt ccggg
//
LOCUS       DAOPAF010000090         4696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_15188_length_4696_cov_23.683473, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000090 DAOPAF010000000
VERSION     DAOPAF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15188_length_4696_cov_23.683473"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1139
                     /locus_tag="PLQ77_07465"
     CDS             372..1139
                     /locus_tag="PLQ77_07465"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HPL10552.1"
                     /translation="MKIRILGCHGSQLPNYNTTSFLVGKNILIDAGAITQVLTLREQL
                     LIDCILVTHAHLDHVRDIMFLADNLCFAKRKTPLRICSSRGIIENIRRHLFNNVIWPD
                     FTRIPSAQNPTVKFEILSPGRKKTFGDWRVRAIPVHHTVETMGFLMESQGKAALFLGD
                     TGPTHEVWKVAGAAKGLQAIFVETSLPAAMQSLADKTGHLTSVTLAAELKKMKDSRPD
                     VYLYHMKPRYAGTIRREVARIPDRKIHIIQDGQVIRI"
     gene            1155..2432
                     /locus_tag="PLQ77_07470"
     CDS             1155..2432
                     /locus_tag="PLQ77_07470"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPL10553.1"
                     /translation="MNPLKPSAYLAGLNIDVMRFGLEAITGLLERFGFPQNEYPALLI
                     AGTNGKGSTAAMAASILKEAGFRTGLYTSPHLVDVRERIALNGKKIPERDLGSILSEI
                     QAKVCGPVSYFEALTAAAFIYFQRARVDIAVMEAGLGGRLDATNVCRPLVSVITNIGL
                     EHTAYLGRTLTAVAGEKAGIIRQGGVCVTGATQQKVTDHLAFICRQKKSRFFRLGKDF
                     KIIQRPDGRVDYCGIRRKISGLRVALAGAHQLANAALALAALEIVEEHGLKISDAAVV
                     SGLKKTRWEARMEILRDRPVFLLDGAHNPSGVRTLCRALKKYFSRRRLILIFAVLSDK
                     DYRAMLKKIAPLAAVTILPPLKTARAVPAQQLAREMKEMGFKAVAAKTVDEAIHTALG
                     MAQESDVICALGSLYLAGEVKQSFHQLNSCGKA"
     gene            2540..>4696
                     /gene="lptD"
                     /locus_tag="PLQ77_07475"
     CDS             2540..>4696
                     /gene="lptD"
                     /locus_tag="PLQ77_07475"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPL10554.1"
                     /translation="MVMMLGVILWLWPAQGGWTQDLKMPEDAPVQIEADELSYDQEKD
                     LYAARGNVVITYGDGVLTAASAEYDRKSNLATAWGNALLQMARDSLAGDKIVVNVEDK
                     TGVAYNSKAFYARNHFYISGNRIEKTGENTYFIDQPVATTCDGDNPDWEIAGRSMKVT
                     LEGYGLMKGARLRARHLPVFYTPLIAFPAKTKRQSGFLFPYLGYSKDKDGIDIEIPFF
                     WAINPQMDATFFSRYMEKRGYKQGAEFRYYAGGKSFGTLYGDFMEDNKHATETAAVGS
                     QSRDWQGMHRRWSYYVNHQTNFDSQFYIRTDIRRVSDSWYFRDFNAHNYYLANYATTE
                     TDPFRKVPFQGNESLPSLESSARVYKGWNNFSLMARVSSTDDFSAVNNDRTLQQYPEI
                     VFTGMKQQFFSTPLYYDFTGSYDYFYRGEGQKGHYADIAPTISLPLNISRYAKVTPQI
                     TLREVYWNRDDSQANSDNKSGNRTVYNAGLSISSRISRVFAVKMQNWDKIKHEIKPEL
                     FYSYIPGVNQDHLPDYIPWMNSFMAGSLSSNIANTNVLTEQNAVAWALTNTLTARAKS
                     KSGLHSYFELLRFKLFQVYDINEAKKDMAGATAERRPVSDLGIEFDLKPHPFITFAAR
                     NKYNPYVGWKEMNYDLGLSDWRGDYLTVGYRYTLDSLEEINMVLKVNLTRGFSGNLVL
                     RRDQFNDRIVENTVGLTYQAQCWGVGVDYTKTYDDER"
BASE COUNT         1113 a         1255 c         1332 g          996 t
ORIGIN      
        1 gaacagcgcg cctactgcaa ggaaatcatg ctgatcaagg cccagggaga tgacgcccag
       61 tatgaacggg tctggaacaa gtatgttttt gccctgaacg gcgaatacgc gatgaatgaa
      121 tagacgggga ttttcaggac cgtcgccgcg ctctaaagaa aagaagggga gggaagggat
      181 gcttctctcc cctttcgtta tggaacaggt ttttcgggga aagcagacgg gatttcgggc
      241 cggtccggag cgtgggacat ggatcgaaaa gcctttaggc gtcatgcgct tacaaaatca
      301 ttgcctgcct gccgaaggta tgatagcgtc ccgcgtcaag cggctcatag attgaaaagc
      361 gggagcttga catgaaaata cggattctgg gttgccacgg ctcgcagttg ccgaattaca
      421 atacgaccag ttttctcgtc ggaaaaaaca tcctgatcga cgcgggcgcc atcacgcagg
      481 tcctgacgct tcgggagcag cttctcatcg attgcattct ggtcacgcat gcccatctgg
      541 accacgtgcg cgacatcatg tttctggcgg acaatctctg cttcgcgaag cgaaagaccc
      601 ctcttcggat ttgcagcagc cggggtatca ttgaaaatat ccgccgccat cttttcaata
      661 acgtcatctg gcctgatttt accagaatcc ccagtgctca aaatcccacg gtcaaatttg
      721 agattctctc tcccggccgg aaaaaaacat tcggcgattg gcgggtgagg gccattccgg
      781 ttcatcatac cgtggaaacc atggggtttt tgatggagtc gcaggggaag gcggctcttt
      841 tcctgggaga tacggggcct acccacgagg tatggaaagt cgccggggcc gcaaaaggcc
      901 ttcaggcgat ttttgtggaa acgtctctgc ccgccgcgat gcagagcctc gcggataaaa
      961 caggccacct cacctccgtg acgcttgcgg cggagctgaa aaaaatgaaa gactcaagac
     1021 cggatgttta cctttaccac atgaaaccga gatacgcggg aaccattcgc cgcgaagtcg
     1081 caaggatacc ggatcgaaag attcacatca ttcaagacgg ccaggtcatc cgcatttaat
     1141 ttacgggcta ccccatgaat cccttgaagc cctccgcata tcttgccggc ctgaacatcg
     1201 acgtcatgcg attcggcctg gaagcgatca ccgggcttct ggagcggttc ggcttcccgc
     1261 agaacgaata tcccgcgctg ctgatcgcgg gcacgaacgg caaagggtca acggccgcga
     1321 tggccgcttc cattctgaag gaggcgggtt tccggaccgg gctctacacg tcgcctcacc
     1381 tggtggatgt ccgggagcgg attgcgctga acggaaaaaa aattccagag cgcgatctgg
     1441 gcagcatcct ctcggaaatt caggccaaag tctgcggacc cgtatcctac tttgaagcgc
     1501 tcacagcggc ggcgtttatt tattttcagc gggcgcgggt ggacatcgca gtgatggaag
     1561 caggccttgg cggcaggctc gacgcgacca atgtatgccg gccgctggtt tccgtcatta
     1621 ccaatatcgg gcttgagcac acggcttatc tgggccgcac cctgacggcg gtggccggag
     1681 aaaaggccgg aatcatccgg caggggggag tctgcgtaac cggcgcaaca cagcagaagg
     1741 taacggatca tctggccttc atctgccgcc aaaaaaaatc ccgtttcttt cgtcttggaa
     1801 aggattttaa aatcattcag cggccggacg gccgcgtgga ctattgcgga atccgccgga
     1861 agatcagcgg tcttcgcgtt gcgcttgcgg gcgcccacca gttggccaac gcggcgctgg
     1921 cgctggccgc cctggaaatc gttgaggaac acggtttaaa gatcagcgat gcggccgttg
     1981 tttccggcct gaagaaaacg cgctgggagg cccgcatgga aatccttcgc gaccggccgg
     2041 tttttttgct ggacggcgcg cataatcctt ccggcgtccg gactctgtgc cgcgcgctga
     2101 aaaaatattt ttcccgaagg cgtctgattc tgatctttgc cgttttgtcg gacaaggatt
     2161 accgcgccat gctgaaaaag atcgcccctt tggccgccgt tacgattctg ccgccgctga
     2221 aaacagcgcg ggccgtgccg gcgcagcagc tcgcccgcga gatgaaggaa atgggattta
     2281 aggcggtggc ggcaaaaacg gtcgatgagg cgatccacac ggcgctgggg atggcgcagg
     2341 aaagcgatgt gatctgcgcc ctcgggtctc tttatcttgc cggagaggtc aaacagtcgt
     2401 ttcatcaatt gaattcttgt ggtaaagcct gacgcgaata agtgaaaagt ggaaagtgga
     2461 aagtgaagag gggtttttcc tttcctgcgt gtcataaaaa ccggggataa agaatttgca
     2521 ggttttgctg agacgacata tggtcatgat gctgggggtg atcctctggc tctggcccgc
     2581 acagggcggc tggacccagg acttgaagat gcccgaagac gcgccggtgc aaatcgaagc
     2641 ggatgagctg tcctacgatc aggaaaagga cctgtacgct gcgcggggga atgttgtgat
     2701 cacttacggc gacggtgttt tgaccgctgc cagcgccgaa tatgaccgca aaagcaatct
     2761 ggcgaccgcc tggggaaacg ccctgcttca aatggcccgg gattcccttg cgggggataa
     2821 aatcgtggtc aacgttgagg ataaaaccgg cgtggcctac aactccaaag ccttttacgc
     2881 gcgcaatcat ttctacatca gcggcaacag aatcgaaaaa acgggcgaga acacctattt
     2941 catcgatcaa cccgtggcga ccacctgcga cggggacaac ccggactggg aaattgccgg
     3001 acgttcgatg aaagtgacgc tggaagggta cggattgatg aaaggcgccc ggctgagggc
     3061 gaggcatctg ccggtctttt atacgccgct catcgcgttt cccgcaaaaa ccaagcgcca
     3121 gtcgggattt cttttcccct acctcggtta ttccaaagat aaagacggca tcgatatcga
     3181 aatccccttt ttctgggcga tcaatcctca gatggatgcg acgttctttt cgcgctacat
     3241 ggaaaaaagg ggatacaagc agggggctga attccgctac tatgcgggcg gcaaatcctt
     3301 cggcactctt tacggggatt tcatggaaga caacaagcac gccacggaaa ccgccgcggt
     3361 cggcagccag agccgcgact ggcagggaat gcacaggcgc tggtcttatt atgttaatca
     3421 ccagacgaat tttgattccc agttttacat ccggacggac ataaggcgcg tttccgattc
     3481 ctggtatttt cgggatttca acgcccacaa ctactacctg gccaactacg cgaccaccga
     3541 gaccgacccg tttcgcaagg ttccctttca gggcaacgag tctctgccgt ccctggagtc
     3601 gtccgcgcgg gtgtacaagg gatggaacaa cttcagcctg atggcccgcg tcagttccac
     3661 ggacgacttt tccgccgtca acaacgaccg gactcttcag cagtatccgg aaatcgtgtt
     3721 cacgggcatg aaacagcagt ttttttccac ccccctgtat tacgatttta cgggcagcta
     3781 tgattacttt taccggggcg agggacaaaa ggggcattat gcggatatcg cgccgacgat
     3841 ttccctgcct ttgaatatct cccgctacgc gaaggtcacc cctcaaatca ccctgcggga
     3901 ggtttactgg aaccgcgacg attcgcaggc caattccgac aataaaagcg ggaaccgcac
     3961 cgtgtacaat gccggcctga gcatcagcag ccggatttcg cgcgtgtttg cggtcaaaat
     4021 gcaaaactgg gacaaaataa aacatgaaat caagcctgaa ctcttttatt cgtatattcc
     4081 cggggtgaac caggaccatc ttcccgatta cattccctgg atgaattcgt tcatggccgg
     4141 ctccctgtcg tccaatattg ccaataccaa tgttttgacg gagcaaaacg cggtggcctg
     4201 ggcgctgacc aacacgctga ccgcaagagc gaagagcaag tccggattgc acagctattt
     4261 tgaattattg cgttttaagc tgtttcaggt ctatgacatc aacgaggcca aaaaagacat
     4321 ggccggcgcc accgcggaaa ggcggccggt ctcggacctg ggaatcgagt ttgacctgaa
     4381 gccgcacccc ttcattacct ttgccgcgcg aaacaaatac aacccgtatg tcggctggaa
     4441 agagatgaac tacgatctgg gtctcagcga ttggcgggga gactatctga cggtaggata
     4501 ccgctatact cttgattctc ttgaggaaat aaatatggtg ctgaaagtaa acctcacccg
     4561 cgggttcagc ggcaacctgg ttctcaggcg ggatcagttc aatgaccgga tagtggaaaa
     4621 cacggtcggc ctcacctatc aggcgcagtg ctggggcgtg ggagtggact acaccaagac
     4681 ctacgatgac gaaaga
//
LOCUS       DAOPAF010000091         4682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_15249_length_4682_cov_57.874433, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000091 DAOPAF010000000
VERSION     DAOPAF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15249_length_4682_cov_57.874433"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1157)
                     /locus_tag="PLQ77_07480"
     CDS             complement(162..1157)
                     /locus_tag="PLQ77_07480"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPL10555.1"
                     /translation="MPNANAAGKSARKKENTMTIVEVSPRDGFPEICNGLSTEDKIHY
                     INKLSETGLKKIECASFTHPRLLPEGYDAEKLVSGIAKKPEVTYVGLVPNEIGCRRAL
                     VTKIDELLVLLSASDTFNRINIGKSLRESLNKVLPTLLDAAARHGRSVRIYLMTAFGC
                     PYTGRVRPEEVAELLLKLSYMGASEVVLVDSTGMADPKSAKELIRALLELKLDIQLAV
                     HFHNTRGTALANCVAAYDEGIRIFDASLGGMSNTPYGVSELDIGYWNVPTEDIVHLFE
                     AMGIQTGIDLEKLLDCVRLAEKMAGKPLPGHILRARPATRITEIPRHLKEPRLLD"
     gene            complement(1226..2278)
                     /locus_tag="PLQ77_07485"
     CDS             complement(1226..2278)
                     /locus_tag="PLQ77_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPL10556.1"
                     /translation="MTAGTKKSQAAQPRGKEESPTNADATLAGSAFDELIGDDEAFRR
                     ALVSAGRAAQSDISVLIIGESGTGKEILARAIHQTSRRKNKQLVDVNCAAIPDSLIES
                     ELFGYEKGAFTGARTEGRKGYFDEAHEGTILLDEIGDSSLSVQAKLLRVLESGTFKRV
                     GGIRNVRVDVRVISSTNQDLADLIGQKKFREDLFFRLNTFTIPLPPLRERRGDIDLLV
                     EHFLKASGAAQKQKYRFSPEALAMLHAYRWPGNVRELKGVVSYALNMTQSPVIDAGSL
                     PNFLVSESRSRDGAAPPAAESGAASLNLTQAVRDLERRLITEALAASSNRSEAIRALG
                     ISRRTFYLKLKEYGLE"
     gene            2469..4553
                     /locus_tag="PLQ77_07490"
     CDS             2469..4553
                     /locus_tag="PLQ77_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPL10557.1"
                     /translation="MAEIIGFSKEGPVGIITVSNPPVNALSQAVRSGIKAGIEKGIAD
                     AAVSAMVIICEGRTFIAGADIREFGRPPMEPHLPDVCQFIEDSPKPVIAAIHGTALGG
                     GLEIALSCHFRIALASAKVGLPEVKLGLLPGAGGTQRLPRVAGVQAALEMITSGTPVA
                     AARAKAMGILDEVVEGDLKAAALAFAKKVVLEKSPIRKVSQLQAKADSPSVFEDFAKA
                     NARKFRGFMAPFKCIEAVKAATELPFPEGMKREREIFVELHDSDQSKAQRHVFFAERE
                     VVRIPGLPDDQPTREIKSVGVLGAGTMGGGIAMCFANAGIPVMLLDVKQEFIDRGMAV
                     IKKNYANTVAKGSLKQEVMEKRLALIQPTLAYEDLKDVDLVIEAVFEDMAVKKEVFTK
                     LDAVCKPGAILASNTSYLNVDEIAAITGRPQDVMGMHFFSPANVMRLLENVRGAKTSP
                     EVYATAMKTGKKIGKVPVLVGVCDGFVGNRMLAKRTRECGFMLEEGALPWQIDRVIYN
                     FGFPMGPYAMGDMAGLDIGWRNRKAKFDSLSPREQKNNILDKICEMGRYGQKTGSGFY
                     KYDEKRNATPDPMIEELIINHSKEVGIARRTITDQEIIERAIYSMINEGAKILEEGIA
                     ARPLDIDVVWLYGYGFPVYLGGPMFYADTIGLGKVYDAVLKYRDLVGAEYWTPAPLLE
                     KLAKEGKGFYSK"
BASE COUNT          988 a         1317 c         1365 g         1012 t
ORIGIN      
        1 agcagaaaag gggacggttc tatttatttt gctattagca gaaaagggga cggttctatt
       61 tattttgcta tttattttga gagatgagca acgaaagata gtttttcctt cacgcttcag
      121 tgaaaaaata gaaccgtccc cttttttgtc ttttttgtcc gtcagtccag cagccgcggc
      181 tccttcaaat gccgtggaat ctcggtgatg cgggtcgcgg ggcgcgccct cagaatgtgg
      241 ccgggcaaag gctttcccgc catcttttcc gccagccgga cgcagtcgag gagcttttcc
      301 agatcgatgc ccgtttggat tcccatggct tcaaacagat gaacaatatc ttccgtcgga
      361 acgttccagt atccgatatc cagctcgctc accccgtaag gcgtgttgct cattcccccc
      421 agcgacgcgt cgaaaatgcg gatgccctca tcgtaggcgg ctacgcaatt ggcgagggcc
      481 gtcccgcgcg tattgtgaaa atgcaccgcg agctgaatgt ccagtttcag ctccaggagc
      541 gcccggatca gttcttttgc cgatttggga tcggccatgc cggtcgaatc caccagaaca
      601 acttcggagg cgcccatata gctgagtttg agcagaagct cggcgacctc ctcggggcgg
      661 acgcggcccg tgtaggggca tccgaaggcc gtcatcaggt aaatccgaac gctccggcca
      721 tgccgggccg cggcgtcaag aagcgtcggc agcaccttgt tgaggctctc cctcaaggat
      781 tttccgatgt tgatgcggtt gaaggtgtcg ctggcggaaa gcagaaccag cagctcgtcg
      841 atcttcgtca ccagggcccg ccggcagccg atttcgttgg gcaccagccc cacgtaggtc
      901 acctccggct tcttggcgat gccggaaaca agtttttcgg cgtcgtaccc ttcaggaagc
      961 aggcgcggat gcgtgaagga ggcgcactcg atcttcttca ggcccgtttc cgacagtttg
     1021 ttgatgtaat ggattttatc ttcagtggac aggccgttgc atatctcggg aaagccgtcc
     1081 cgcggggaca cttccacgat ggtcatcgta ttttcttttt ttctggcgga ttttcccgcc
     1141 gcattggcat ttggcataac cgccccaggc cggagaaagt ttttttaaac atgttgcata
     1201 attcattcca gccggccgga tctttttatt ccaatccgta ttccttcaat ttcaaataga
     1261 aggtgcgccg gctgatccca agggcccgga tggcttccga ccggtttgaa gaagcggcta
     1321 gcgcctcggt gatgagcctt cgctccaggt cccggacggc ctgggtcaga ttgagagagg
     1381 cagccccgct ttcagcggca ggcggcgcgg ctccgtcccg actgcgagat tccgaaacca
     1441 ggaaattggg cagggaaccg gcatcgatga cgggcgactg cgtcatgttc agcgcgtagc
     1501 tcacaacgcc tttgagctcc cggacgtttc ccggccagcg gtacgcatgc agcatcgcga
     1561 gcgcttcggg cgaaaaccgg tatttctgtt tctgcgcggc gccgctcgcc ttcaggaaat
     1621 gctccaccag cagatcgatg tctcctcttc tctcgcgcag cggaggcagc gggatcgtga
     1681 aggtattgag gcggaaaaaa agatcttcgc ggaatttctt ctgcccgatc agatccgcca
     1741 gatcctgatt ggtcgaggag atgacccgga cgtccaccct gacgttcctg attccaccga
     1801 ctcttttaaa cgttccggat tccaggaccc tgagcagttt ggcctggacg gacagggagg
     1861 aatctccaat ttcgtcgagc agaatggttc cctcgtgcgc ctcgtcgaaa tagcctttgc
     1921 gtccctcggt ccgcgcgccg gtaaacgcgc ctttttcata gccgaaaagc tcgctttcaa
     1981 tcagggaatc gggaatggcg gcgcagttca cgtcgacgag ctgcttgttt ttgcggcggc
     2041 tggtctggtg aatggcgcgc gccaggattt ccttgcccgt gccgctttcc ccgatgatca
     2101 gcacggaaat atccgattgg gccgcccgcc cggcgctcac cagcgccctg cgaaaggctt
     2161 catcgtcgcc gatcagttcg tcaaacgcgc ttccggccaa cgtggcatcc gcattcgtcg
     2221 gggattcttc ttttccgcgc ggttgagccg cttgcgattt tttggttcca gccgtcatgg
     2281 gttcggtttt ctttcctgca tgtctatccg gtatacaaat ttcgttctga atgtatataa
     2341 atcatacatc cctgtcaaac agtttttacc gcgcgcggat cagcgactgc gccgctattg
     2401 ccttttttca ctggtatggt atttgctgta ttcaacggaa aacaatctgc aacaaaagga
     2461 gagtacttat ggcagaaatc atcggatttt caaaagaagg ccccgtcggc atcatcacgg
     2521 tcagcaaccc gccggtgaac gccctgtcgc aggcggttcg ctcgggaatc aaagcgggga
     2581 tcgagaaggg cattgcggat gccgccgttt cggcaatggt gatcatctgc gagggccgga
     2641 cgtttatcgc gggcgcggac attcgggaat tcggccgccc gccgatggag ccgcacctgc
     2701 cggacgtctg ccagttcatt gaagattctc caaagccggt tattgcggcg attcacggaa
     2761 cggccctggg cggaggcctg gaaatcgctc tttcctgtca tttccgcatt gcgcttgcct
     2821 ccgcgaaagt aggtcttccc gaagtcaaac tggggcttct gcccggcgcg ggcggaacgc
     2881 agcggctgcc gcgtgtggca ggcgtccagg cggcgctgga gatgatcacc tccggaacgc
     2941 cggtggcggc ggccagggcc aaagccatgg gcattctgga tgaagtcgtt gaaggcgatc
     3001 tgaaagcggc ggcgcttgcc tttgccaaaa aagtcgtttt ggagaaaagc cccatacgca
     3061 aagtaagcca gcttcaagcc aaagccgatt ctcccagtgt ttttgaagac ttcgccaaag
     3121 ccaacgcccg caagttccgc ggtttcatgg cgcctttcaa gtgcattgaa gccgtcaagg
     3181 ccgccacgga actgcctttc ccggaaggca tgaaacggga aagggaaata ttcgtagagc
     3241 tgcacgattc cgatcagtcc aaggcccagc ggcatgtttt ctttgccgag cgtgaagtcg
     3301 tccgcattcc cggactgccc gacgatcagc cgacccgcga gattaaatcc gttggcgtcc
     3361 tgggcgcagg cacaatgggc ggcggcattg ccatgtgctt tgccaatgcg ggcatccccg
     3421 tcatgctgct ggacgtcaag caggaattta tcgaccgggg catggccgtt attaaaaaga
     3481 attacgccaa caccgtcgcc aagggctcgc tcaagcagga ggtcatggaa aaacgcctgg
     3541 cgctgatcca gcccacgctg gcctatgaag acctgaaaga tgtggacctc gtcatcgaag
     3601 cggtctttga agacatggcc gtcaagaagg aggtcttcac gaagctcgac gcggtttgca
     3661 aacccggcgc gattctggct tcgaacacct cctacctcaa cgtcgatgaa atcgccgcca
     3721 tcacagggcg gccgcaggat gtcatgggca tgcatttctt cagccccgcc aacgtcatgc
     3781 ggctgctgga aaacgtccgg ggcgcgaaga cttcccccga ggtttacgcc acggccatga
     3841 agaccggaaa aaagatcggc aaggtgccgg tcctggtcgg cgtctgcgac ggattcgtcg
     3901 gaaaccggat gctggccaag cgcacccgcg aatgcggctt catgctggaa gaaggagcgc
     3961 ttccctggca gatcgaccgc gtcatttata atttcggctt cccgatgggc ccctacgcga
     4021 tgggcgacat ggcgggcctc gacatcggct ggcgaaaccg caaggccaag ttcgactcgc
     4081 tttcgccgcg cgaacaaaag aacaacatcc tggacaaaat ctgcgaaatg ggacgctacg
     4141 ggcagaaaac gggttcgggc ttctacaagt atgacgagaa gcgcaacgcg acgcccgatc
     4201 cgatgattga agagctgatc atcaaccact cgaaggaagt cggcatcgcg cgtcggacca
     4261 tcacggatca ggaaatcatc gaacgcgcga tttactccat gatcaacgaa ggcgcgaaga
     4321 tcctggagga agggatcgcc gcgcggcccc tggacatcga tgtggtctgg ctctacggct
     4381 acggctttcc ggtgtacctg ggcggcccga tgttttacgc cgatacgatc ggactcggca
     4441 aggtttacga cgcagtcctg aagtaccggg atctggtggg cgccgaatac tggacgcccg
     4501 cgccattgct ggaaaagctg gccaaagaag gaaaaggatt ttactccaaa taaaaattcg
     4561 gaacggggaa aagggagggg atgatgaagt atcaggaggt ttacgcgcat tcgatttctg
     4621 agccggacgc cttttggggc aaagccgggg aagccataga gtggacgagg aggtgggaca
     4681 aa
//
LOCUS       DAOPAF010000092         4413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_16331_length_4413_cov_48.946306, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000092 DAOPAF010000000
VERSION     DAOPAF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_4413_cov_48.946306"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ77_07495"
     CDS             complement(<1..346)
                     /locus_tag="PLQ77_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPL10558.1"
                     /translation="MGLIRLLISDPAAFVMLAVLLLYSVIFHELAHGWVALRMGDETA
                     KWLGRLTLNPIKHLDPIGSLMLLVIGFGWAKPVPVNLENIPAPQRRKGLILVSAAGVT
                     ANMLIAFFALLLW"
     gene            527..2680
                     /locus_tag="PLQ77_07500"
     CDS             527..2680
                     /locus_tag="PLQ77_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL10559.1"
                     /translation="MPENPLRLLMNPRSIAVVGASNDFRKMGTIQALSILQDGYSGDV
                     FFIHRKEKTVLGRPAFPAAADLPKAPDLAVLIVPSSSIAGLLADFGAIGTKRAIIITA
                     GFKETGSAGRSMEEKIKSVASRHGIRFVGPNCLGIINSENSLNTTVAPFTTGPGRLGF
                     ASQSGTYVTQALPYLRKNGIRYSKAISLGNEADIDLVDALEYLGEDEQTRAVILYIEG
                     IRDGRRFVEVARRITPRKPVVAQYVGGSASGARAGASHTGAMSGPDFLYDGIFKQAGI
                     LRVPTIEDLYAHGWTLATQPPLRGRRVGVVTNSGGPGTGIAYMCDHEGLEVPRFSESL
                     QRDIRPLIEAHASAANPVDLTFDLGMRKLAVTLPEMIMKSGEVDALILHGIMHSGFMV
                     EIFEHLRDYLQGVSYEAFLKGTRSVKEDAFQLPGKYPFPFLISSFFDDTDDCTRGYLD
                     AGVPVFRSPENAARALGSLHRCRLIRERVLSNAPPLSQAQPAALEILGRAKAEGRGAL
                     DECEAKQVLAAYGVPVTREAKAVTCDDAVEAAKQIRYPVAVKACSWEILHKSGRGLIA
                     LNLKKEAQVQEAFDGIQKAAGKPTPVLVQEMVFGSREFLVGMTRFPGFAPCVVFGLGG
                     VWTEIHRDMTLRQSPLSDSDAAEMLADLRSSRLLGEYRGMPEVKRGALAGILQAVGTI
                     ALLHPEIAEIDINPVMIRGDEPVAADALIGLKAED"
     gene            complement(2803..3969)
                     /gene="rpsA"
                     /locus_tag="PLQ77_07505"
     CDS             complement(2803..3969)
                     /gene="rpsA"
                     /locus_tag="PLQ77_07505"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPL10560.1"
                     /translation="MEDTKTFAEMLEESYAAPKRFAVGEKAEATIVKISPEWVFIDLG
                     AKSEGYIDKKEFLDEEGNLTAREGDSVTAYFLSSRHSERLFTTKLLSRKSVDDFLANA
                     YAHRIPLEAAVEKEVKGGFSVKISASASGFCPFSQMELRRIESAADYIGKKFDFLVIE
                     YGENGRNIILSRRPLLEKIEQEKKSALKESLTKGMTVQGVVTSVRDFGAFVDIGGVQA
                     LLPVSEMTWGRVEDAKSLYKPGDKIEAVVINIDWENDRVTLSFKDTLPDPWDDVVRNY
                     VEGSIHRGRVSRLTDFGAFVTLEAGVDGLLHISKLGRGKKIKHAQDVLAKDRDIDVKI
                     EKIDREHKKIALDLASGGAENGGPREESGDDYKSYLPKSPKTMGTLGDLMKKKK"
     gene            4146..>4413
                     /locus_tag="PLQ77_07510"
     CDS             4146..>4413
                     /locus_tag="PLQ77_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPL10561.1"
                     /translation="MRFEISRGGSGLTYEIRNIVMIAKKMQEYGLKIIWENIGDPVQK
                     GENIPDWMKEVLIDILREDMSYAYSPTKGVDATRAFLAERTNARG"
BASE COUNT          913 a         1262 c         1299 g          939 t
ORIGIN      
        1 gccacagcaa aagcgcgaaa aaagcgatga gcatgttggc cgtcacgccg gcggctgaaa
       61 ccagaatgag ccctttgcgc ctttgcggtg cgggaatgtt ttccagattg accgggaccg
      121 gtttggccca gccgaatccg atgacgagca gcatgagcga cccgatggga tccagatgct
      181 tgatgggatt cagcgtcagc cttcccagcc atttggccgt ttcgtcgccc attcgcaagg
      241 cgacccagcc gtgcgccagc tcgtggaaga tcaccgagta caacagcaga acggccagca
      301 tcacgaatgc ggccggatcg ctgatcagca ggcggattaa acccatcacc ccttctccct
      361 tcttgatcgg gttcccgaat gattgtctcc gggaatttca acctgacgga aagcataaat
      421 catgaagacg cgatattcaa ccggagtttt catcttgaca gacaaggcgt ttaatttgta
      481 ggaatgttcc gcacattcca tcaccttcag aaaaggaaga ggaaccatgc cggaaaatcc
      541 tttgcgtctg ctgatgaatc cgcgctccat agcggtggtc ggcgccagta atgatttcag
      601 gaaaatgggc accattcagg ctttgagcat cctccaggac ggctattcgg gggacgtgtt
      661 tttcattcac cgcaaggaaa aaaccgttct cgggcgcccc gcttttccgg cggcggccga
      721 tctgccgaaa gcgccggatc tggcggtcct gatcgtgccg agctcctcca tcgcggggct
      781 gcttgcggat ttcggcgcca tcggaacgaa gcgcgccatc atcattaccg caggcttcaa
      841 ggaaaccgga tcggccggcc ggagcatgga agaaaagatc aagtccgtcg ccagccggca
      901 cggcatccgg tttgtgggcc ccaactgcct ggggatcatc aattccgaaa attcgctcaa
      961 caccacggtg gccccgttta cgaccggccc gggacgcctg ggattcgcgt cgcagagcgg
     1021 cacctatgtg acgcaggcgc tgccgtatct gcgcaaaaac ggcatccgct acagcaaggc
     1081 gatcagcctg ggcaacgagg ccgacatcga tcttgtggac gcgctggagt atctgggcga
     1141 ggatgaacag acgcgagccg tgattcttta cattgaaggc attcgggacg gccggcggtt
     1201 cgtggaggtt gcccgccgga tcacgccccg caagcccgtc gtcgcgcagt atgtgggtgg
     1261 ttcggcctcg ggcgccaggg caggcgccag ccacacgggc gccatgtccg ggccggattt
     1321 tctctacgac ggcattttca aacaggcggg cattctgcgc gtcccgacga tcgaggatct
     1381 ctacgcgcac ggctggacgc ttgccacgca gccgcccctt cgcggaaggc gggttggcgt
     1441 tgtgaccaat tccggcggac ccggtacggg cattgcctac atgtgcgacc atgaagggct
     1501 ggaggttcct cgcttctccg agagcctcca gcgagacatc cggccgctga tcgaagcgca
     1561 cgcctcggcc gccaacccgg tggacctgac gtttgacctc ggcatgcgaa agctggccgt
     1621 aacgctgccg gagatgatta tgaaaagcgg cgaggtggac gcgctgattc tgcacggcat
     1681 catgcactcg ggcttcatgg tcgaaatttt cgaacacctg cgcgattatc ttcagggtgt
     1741 atcctacgag gcttttctga aaggtacgag atccgtcaag gaagacgcat ttcagctgcc
     1801 gggcaaatac ccgtttccgt ttttgatctc ctcgttcttc gacgacaccg acgactgcac
     1861 gcgcggctat ctggacgccg gcgttccggt gttccgctct ccggaaaatg ccgcccgcgc
     1921 gctcggttcc ctgcaccgct gccggctgat tcgggagaga gttctttcga atgcgccgcc
     1981 gctgtcccag gcacagcccg ccgcgctgga aattctggga cgggcgaaag ccgaagggcg
     2041 cggcgcgctg gatgaatgcg aggccaagca ggtgctggcg gcctacggcg tgcccgttac
     2101 ccgcgaggcg aaggccgtga cctgcgatga cgccgtggaa gcggccaagc aaattcggta
     2161 tcccgtagcc gtcaaggcgt gcagttggga aatcctgcac aaaagcggca ggggccttat
     2221 tgcgctgaat ctcaaaaaag aggcgcaggt tcaggaagcg tttgacggca ttcaaaaggc
     2281 ggcggggaag cccacgccgg tgctggtgca ggaaatggtc ttcggcagcc gtgaatttct
     2341 cgtcggcatg acgcggttcc ccggatttgc gccttgcgtg gttttcggtc tgggcggtgt
     2401 ctggacggaa atccaccgcg acatgacttt gcggcagtcg cctctgtccg attcggacgc
     2461 ggccgaaatg ctggccgacc tgcgcagctc gcgcctgctg ggcgaatacc ggggcatgcc
     2521 ggaagtgaag cgcggggccc tggccgggat tcttcaggcg gtggggacca tcgcgcttct
     2581 gcacccggag atcgccgaga tcgacatcaa tcccgtcatg attcgaggcg atgaaccggt
     2641 tgcggcggat gcgctgatcg gattaaaggc tgaagactga aggtacgagg gacagtgcgc
     2701 tatcgttttg tgaatagtgg atggtggata gcggatggtg aagaaaggcg agaggcaatg
     2761 ggctataggc gataggaaaa aagctcattg ctcattgctt atttacttct ttttcttcat
     2821 caaatctccc agcgtcccca tggttttggg cgacttgggc aggtagctct tgtaatcgtc
     2881 tcccgattct tcccgtgggc cgccgttttc cgcgccgccg gaagccagat caagcgcgat
     2941 ttttttgtgc tcgcggtcaa tcttttcaat cttcacgtcg atgtcgcggt ccttggccag
     3001 cacgtcctgg gcgtgcttga tctttttgcc ccggcccagt ttggagatgt gcaaaagccc
     3061 gtccacgccg gcttccagcg tgacaaacgc gccgaaatcc gtcagccgcg aaacccggcc
     3121 cctgtggatg cttccttcga cataattgcg gacaacgtcg tcccacggat ccgggagcgt
     3181 atccttgaaa gacagtgtga cgcggtcgtt ttcccagtca atgttgatga cgaccgcctc
     3241 aatcttgtcg cccggcttgt acagggattt tgcgtcctcc actcttcccc aggtcatttc
     3301 cgaaaccggc agaagcgcct ggacgccgcc gatgtccaca aacgcgccga agtcgcgcac
     3361 cgaagtgacc accccctgca cggtcatgcc ttttgtcagg gattccttca gcgcggattt
     3421 cttttcctgc tcgatttttt ccaggagagg gcggcgcgac aggatgatgt tgcggccgtt
     3481 ttcgccgtat tcgatcacga gaaagtcgaa ttttttgccg atgtaatcgg cggcgctctc
     3541 gattcttcga agctccatct gcgaaaaggg gcagaaaccg ctcgcgctgg cgctgatttt
     3601 taccgaaaat ccccccttga cctccttttc aacggcggct tccagcggaa tccggtgggc
     3661 gtaggcgttg gccagaaaat catccacgct cttgcgggaa agcagtttgg tcgtaaacag
     3721 cctttcgctg tggcgcgagg aaaggaaata agcggtcacg ctgtcgcctt cccgggcggt
     3781 cagattgccc tcctcgtcga gaaattcttt tttatcgata tagccttcgc tcttggcgcc
     3841 cagatcgatg aagacccatt ccggggaaat cttgacgatg gtcgcctccg ctttctcgcc
     3901 cacggcgaag cgtttggggg cggcgtagga ctcctcgagc atttcagcaa atgtttttgt
     3961 atcttccatg agaaacattc cttcggaatt ttaatgtgcg cgcctcaata tcatgaaacg
     4021 tttcgtttgt ccttctaaaa ttaccgcttg tcccgtaaaa cagcgcgaaa actaaaaaaa
     4081 tctggataaa tcctgaaata tgtattatgc aggtactcaa aaatatccaa ggagttaagc
     4141 aacttatgcg ttttgagatt tcccgcggcg gcagcggcct cacctacgaa atccgcaata
     4201 tcgtgatgat cgcgaaaaag atgcaggaat acgggctgaa aatcatctgg gaaaacatcg
     4261 gcgacccggt ccagaagggg gaaaacatcc ccgactggat gaaggaagtc ctcatcgaca
     4321 tcctcaggga ggacatgtcc tacgcctatt cgccgacgaa aggcgtggac gccacgcgcg
     4381 cttttctggc ggagcgcacc aacgcgcggg gaa
//
LOCUS       DAOPAF010000093         4407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_16355_length_4407_cov_58.777344, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000093 DAOPAF010000000
VERSION     DAOPAF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16355_length_4407_cov_58.777344"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1732
                     /locus_tag="PLQ77_07515"
     CDS             128..1732
                     /locus_tag="PLQ77_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10562.1"
                     /translation="MFFRKAPVCVPENNRRRAAQNDLSALSLILILGLAIRLFAWGQT
                     DIMNSDGTVYIQQARAIYFGLWDAVTSCTIRYPYINSLLIAGFYALCKNWVTAATAVS
                     VFFGVLTLIPLYGLSRNFFSAGVSFLIALIHAMNPMLVDGSVDIIRDPVCWFFTLCGL
                     WVITRPQGRLPSGLLLACVAFILAIWARIESVIFFLVTPLWLLLDGRERPWRRILIFV
                     APAALLLLMGFGIQWLWRPDSVSMYRLQDIPLWLTRSFAGYTRLNATLREMIFQSPQS
                     LSPEFLQNLKDIIWFVGFGVVFQNALETFFYPFVILYLMGLGGLRKRIAADGRIVYLL
                     LLTLTGFLLLYLMVFIDWQMENRWLAIVLFPSFFFLGAGLERLISWLHLRFNLKQGAA
                     IVLIGLAVLAATLPKDIKPRGEDKVIYRTIGETIADREGSGKKIEIMTVGVGSRWILF
                     YANLGFRGAPCPDEFYHYREWDGREYGPFVERMKAKGMKYLVWEERNWPADWFDFLLS
                     PYGKDFVLLGQWSHPDTGKILLFRAR"
     gene            1739..2887
                     /locus_tag="PLQ77_07520"
     CDS             1739..2887
                     /locus_tag="PLQ77_07520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL10563.1"
                     /translation="MADRLYSIAVVIPKYGLVGGAEGFAAELTERLARDGRFRFHVFA
                     NRWVARSERIVFHKTPILSFPKFLTTPSFAFFADRQIRRCGIDLIHSHERIFRADLAT
                     LHGVPHRFWVRRVRRKRMSLYDRATVGVEEKLLADPQCRLLPVSGLTREILLKEYPVD
                     PKRVCVVHPGVDPDLFSAPDRAACRADLAGRFGIRPEERVILFVSMNFEIKGLDALMK
                     GLSFLAARGGAPPFRLIVAGKGDVRKHKALAETLGIGERIVFAGVVPGVEIPFLYRAA
                     DLYAMLSRFDTFGMVVLEAMAASLPVLISDRVGARDLVREGINGSVVAADAAPEEIAS
                     RLALLLDGSSREKMGRAARETARQNSWDAVAERVLSVYEELLECRQRR"
     gene            complement(3208..4296)
                     /locus_tag="PLQ77_07525"
     CDS             complement(3208..4296)
                     /locus_tag="PLQ77_07525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL10564.1"
                     /translation="MKIIQNFNYRLPQDTRNILLVQLGDIGDVVWTTPGIWAAKNSIP
                     GSKVSVVVKEGFGGLLEADPSVEQIIEVKRPHGNIVDRTLRQLFFLKDIRSKHFDLVV
                     DLRLGDRGAFISFAAGAPHRVTAHSKENVPFWRKYLFTHGIVPRPPVHTRGAADQSLC
                     ILRELGIDTDNIIPKLWVSDSVKQRVKEMMIHEKIDGLKDWVTVNPFSRWQYKEWNGL
                     KWAQVIDWLWEEFGIAAVIVGAPEERVKAEALIRECKHQAFNLAGRTTLKELAGLLSL
                     SRLHLGVDSAAPHIAAATGAPTVTIYGPSNWRDWAPIGDKHRVILPDMDCVPCRKKGC
                     DHSGYSRCLEALSVDQVKSVIREAVANG"
BASE COUNT          819 a         1291 c         1274 g         1023 t
ORIGIN      
        1 gggtcccatg accatcgcca tgctgatgaa gaacacgctg gacgcggcga ggctggcccg
       61 atagaaccaa aacgttcgtg aaatgaaaat ggaatggatt tgatccgatc cggacaagga
      121 gaaccggatg tttttccgca aggcacccgt ttgcgttccg gagaataacc ggcggcgcgc
      181 cgctcaaaat gatctctctg cgctgtccct gattctcatc ctggggctgg cgatccggct
      241 tttcgcctgg ggccagacgg atatcatgaa ttccgacgga accgtctaca tccagcaggc
      301 ccgggcgatc tactttggcc tctgggacgc cgtgaccagc tgcacgatcc gctatcctta
      361 catcaacagt cttttgatcg ccggttttta cgccctctgc aaaaactggg tcaccgctgc
      421 cacagccgtc tccgtctttt tcggcgttct gacgctcatt cccctctacg gcctttccag
      481 aaacttcttt tctgcggggg tttcgttcct gatcgccctg atccatgcca tgaatcccat
      541 gctcgtggac ggcagcgtgg acatcatccg ggatccggtc tgctggtttt tcaccctttg
      601 cggcctctgg gtcatcacgc ggccccaggg gcggctcccc tccggactgc tgctggcctg
      661 cgtcgccttc atcctggcga tctgggcccg gattgagagc gtcatctttt tcctggttac
      721 ccccttgtgg ctgctgctcg acggcaggga gcggccctgg cgtcgaattc tgatctttgt
      781 ggcgcccgcg gcgctgctgc ttttgatggg tttcggcatt caatggctct ggcgtcctga
      841 tagcgtcagt atgtaccggc tgcaggatat tccgttatgg cttacccggt cttttgccgg
      901 atacacccgg ctgaacgcaa cgctccggga aatgattttc caatctccgc agagtctctc
      961 tccggaattt ctacaaaacc tcaaagacat catctggttc gttggttttg gcgtggtttt
     1021 ccagaatgcc ctggagacct ttttctaccc cttcgtgatc ctctatctta tgggattggg
     1081 aggactccga aagaggatcg ccgcggatgg gcggatcgtt taccttctgc tgctcacgct
     1141 gaccggtttt ctgctcctct atctgatggt cttcatcgac tggcagatgg aaaatcgctg
     1201 gctggccatc gtgcttttcc cttcgttttt cttcctcggc gccggcctgg aaaggctgat
     1261 ctcctggctg catttgcgat tcaaccttaa acaaggggcc gccattgtcc tgataggctt
     1321 ggctgttttg gccgccaccc tgcccaagga catcaagccc cggggcgaag acaaggtcat
     1381 ctaccggacc attggcgaaa cgattgcaga ccgggaggga agcgggaaaa aaatcgagat
     1441 tatgacggtc ggcgtcggct ctcgctggat tctcttttac gcaaatctcg gtttccgggg
     1501 ggcgccctgt cccgacgaat tctaccacta tcgggagtgg gatgggcggg aatatggacc
     1561 gttcgtcgag agaatgaaag caaaggggat gaagtatctg gtctgggaag agagaaactg
     1621 gccggccgac tggttcgatt tcctcctctc gccctacggg aaagacttcg tcctcctcgg
     1681 ccagtggagc caccccgaca cggggaaaat cctcctcttc agggcgcggt gacgagcgat
     1741 ggcggatcgg ttgtacagca ttgccgtggt gattcccaaa tacggcctgg tgggcggggc
     1801 ggaggggttt gccgccgaac tcaccgagcg gctggcccgg gacgggcgct ttcggtttca
     1861 tgtctttgcc aaccgctggg tcgcccgatc cgaacggatc gtttttcaca agacgccgat
     1921 cctgtccttt ccgaagttcc tgaccacccc gagttttgcc ttctttgccg accgtcagat
     1981 acgacggtgc ggcatcgacc tcatccactc ccacgagcgg atcttccggg ccgatctggc
     2041 caccctgcac ggcgttcccc accgtttctg ggtccgccgg gtgcgccgca agcgaatgag
     2101 cctctacgac cgggctacgg tcggcgtcga ggaaaaactg ctcgccgatc cgcagtgccg
     2161 cctgctgccc gtttccggcc tgacccggga gatcctgctg aaagaatacc ccgtcgatcc
     2221 gaagcgggtg tgcgtggttc accccggcgt cgaccccgat cttttttccg cgccggatcg
     2281 cgcggcctgc cgggccgacc tggccggccg cttcggcatc cggccggagg agagggtcat
     2341 tctctttgtc tccatgaact tcgagatcaa ggggctcgat gccctgatga agggattgtc
     2401 gttcctggcc gcccgggggg gcgcgccgcc cttccgtctg atcgtggcgg gcaaggggga
     2461 tgtccggaag cacaaagccc tggccgaaac cctcggcatc ggtgaacgga tcgtctttgc
     2521 gggcgtcgtt ccgggcgtcg aaatcccttt tctctaccgg gccgcggacc tctatgccat
     2581 gctctcgcgc ttcgacacct tcggcatggt ggtgctcgag gccatggctg cctccctgcc
     2641 ggttctgatc agcgaccgcg tcggcgcccg ggacctggtc cgggagggga tcaacggcag
     2701 cgtggttgcg gcggatgcgg cgcctgaaga gatcgccagc cgtctggcgc ttcttctcga
     2761 cgggtcgagc cgggaaaaaa tgggccgggc ggcccgggag acggcccggc agaacagctg
     2821 ggatgcggtc gcggagcgcg ttttatccgt ttacgaggag cttctggaat gccggcagcg
     2881 gcgatgacgg acctttcagg gagcgtggtt tcctgagacc tttgaaaaat gtcatttcga
     2941 ggagcgtgag cctacgcctg atgtcatttc gaaggagcgg agcgactgag aaatcttaag
     3001 acccctcatt gcattcggga cacggatttc tcccttcggt cgaaatgaca aagaagaagt
     3061 cgaaatgaca aaaatggccg ttattcaaag ctctcttcct gcagcgcggc cccaacggat
     3121 taaagcggcc gcctctgccg cccgcctctt ttccggccgt cgcgcggtcg tttcgacccg
     3181 gcgggagggg cgggcggcaa ggccatttca gccgtttgca acggcttcgc ggatgaccga
     3241 ttttacctga tccacggaca acgcttccag gcagcgactg tatccactgt gatcacaccc
     3301 ttttttccgg caaggaacac aatccatgtc cggcaggatc acccgatgtt tgtcgccgat
     3361 gggcgcccag tcccgccagt tcgacggtcc ataaatagtg acggttggcg cgccggttgc
     3421 cgcggcgatg tggggcgccg cgctgtccac gcccaaatga aggcggctca ggctgagaag
     3481 gcctgccaac tccttcagag tggttctccc cgcgagattg aatgcttgat gcttgcattc
     3541 gcgaatcagc gcttcagcct tcaccctttc ctccggcgct ccgacaatga ccgcggcgat
     3601 cccaaattct tcccatagcc agtcgatcac ttgcgcccat ttaagaccat tccattcttt
     3661 gtattgccaa cgggaaaacg ggttgacggt aacccagtct ttcaggccgt ctattttttc
     3721 atggatcatc atttctttca cgcgttgttt gacggagtcg gacacccata atttggggat
     3781 gatgttgtcg gtatcgatgc cgagttctct taggatgcaa agcgactgat cggccgcgcc
     3841 gcgtgtgtga acggggggac ggggcacgat tccgtgtgta aaaaggtatt tcctccaaaa
     3901 cgggacattt tctttgctgt gcgcggttac ccggtgcggt gctcccgcgg caaaggatat
     3961 aaaggcgccc ctgtctccca gacgaaggtc gacaacaagg tcaaaatgtt tagaccggat
     4021 gtccttcagg aaaaataact ggcggagggt ccggtcaacg atatttccat gaggccgttt
     4081 cacttcaata atttgttcaa cggaaggatc cgcctccaaa aggccgccga aaccttcttt
     4141 gaccacaacg gaaacttttg agcctggaat ggaattcttt gcggcccaaa tgccgggggt
     4201 tgtccagacc acgtcgccga tatctcccag ttgaaccagg aggatgttgc gcgtgtcttg
     4261 aggcagtctg taattaaagt tttggatgat cttcataaat aaatcaaagc gccgatgagg
     4321 caacgtcaaa atgtcagcgc cgtttcagac gggggatcgc tgccgcgatt atgctcataa
     4381 aacaactaca ttttccccgt attcttt
//
LOCUS       DAOPAF010000094         4349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_16612_length_4349_cov_70.641826, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000094 DAOPAF010000000
VERSION     DAOPAF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16612_length_4349_cov_70.641826"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..2007
                     /locus_tag="PLQ77_07530"
     CDS             292..2007
                     /locus_tag="PLQ77_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPL10565.1"
                     /translation="MKVKKRLGEMLIDSGLLTEERLQQALVEQKKAGLKLGQYLTRQG
                     VVNEQQVIDMLSRQLNIAKYHPDQYPLDLSLAHYIPIDTAQKYQVAPLRKKGRLLTIA
                     IVDPLDINTLDFIEMLTNSEVEPVVCSERELNQIISSLYGSQSGLGSIMESLEVAAPE
                     ESQEEAAPGKETEVSIASLQDMAGEAPVVRLANSIFAQAIRDGASDVHISPQQNNVQM
                     RFRIDGKLIEVPSPPKALFLPIVARIKILANMDITMSRVPQDGRFTLKMSSKEINVRV
                     SSIPTIYGENLVLRMLDMSGGIYTLDRLGMIPSDREKIEMMSKKPYGLILSTGPTGSG
                     KSTSLYAILSAINKPDINIITLEDPVEYRVNNVRQVQLNRKAGMTFASGLRSILRQDP
                     DVILVGEIRDSETAAIAVQAAQTGHRVLSTVHTNDAAGAVTRFIDMGIEPFLISSVLL
                     VSFAQRLLRTVCPYCKESYQPPQSALDALGITKEQGQNANFARGRGCQQCKNTGYKGR
                     TGLFEVLVNDAMVQDMIMKRYSNQQITKATIEAGTLRTLIEDAAAKVMNGITTPEEAT
                     SAVMT"
     gene            2019..3239
                     /locus_tag="PLQ77_07535"
     CDS             2019..3239
                     /locus_tag="PLQ77_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPL10566.1"
                     /translation="MANYVYSAINEMGKTITDSIEAESEEIVNSMLAARNLIPVRIVR
                     QDKSGFSLSSLSSLRFGASVKTKDLILFTKQFRSMMQAGVPIIRLMQVLVSQTQDKTL
                     KKVATEISQDIKGGMTLYAAMKKHPSVFSPLYLSMINAGEISGSVPEILERLIDIVEH
                     EAKIKKDIKSALQYPIIVVIALVIAFVVLLTFVIPKFVAIFAKAGIALPLPTKIAMIM
                     YQGLANYWHILLGGIIALVVFLRYYIKTPAGRYTLDTFLLKMPLFGSLFQKAAMSRFA
                     AIFAIMQASGVPVMRTMEVVSDTIGNTAISREFDNVRDKIQEGQGISGPLRSAKHFTP
                     MVVDMVAIGEESGNIEEMMRQVSLHYDDEVQYAVKGLADSIGPILMVGLAAVVGFFAL
                     AIFMPMWDLTKMVK"
     gene            3247..>4349
                     /locus_tag="PLQ77_07540"
     CDS             3247..>4349
                     /locus_tag="PLQ77_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPL10567.1"
                     /translation="MKDITSSIKKLSAGRSSPFRVSLFVLIPFIFTGFTVLAFLVAYY
                     AGRASTIQLFLLGGAVIAFTAFSAVVVTFAVLSPVQKFVDEVESSPSFPKPPSESEPE
                     SKPKVNDEIDHFDHVFREVANLISKVDARDRFPGIVGQSRAIRSCLSQVIKVAPTDNT
                     VLLLGESGVGKELFAEAIYQESNRQGKPFVKLNCVAIAAGLLESELFGHEKGSFTGAS
                     AKKTGKFELANEGTLFLDEIGDIQLETQAKLLRVLQEREFQRVGGNETIRVNVRFIAA
                     SNKNLHQMVKEGTFREDLFYRLNVFPIYIPPLRDRVEDIPLIARHILDRMPEKKELSP
                     EAMRELMTWSWPGNIRELKNVLERASVMADQGR"
BASE COUNT         1061 a         1126 c         1188 g          974 t
ORIGIN      
        1 ctctcattgt attcgggaca aggatttctc ccttcggtcg aaatgacaaa gaagatggtc
       61 gaaatgacaa agaagagggt cgaaatgaca aaaaaggggt gcgaaatgaa aatattggcg
      121 gtttttcaaa gctctaaatg tgatgaactc gtaaaaagcc aatttcgcac ccttttcgtc
      181 ataccggcaa aggccggtat ccagtaatta caataagttc tggactccgg ctttcgccgg
      241 agtgacggtt tgaattactt tttacgagac cgtcaaatgt gaggagtgtg gatgaaagtc
      301 aaaaaacgcc tgggtgaaat gctgatcgac agcggtctgc tcacggagga gcgccttcag
      361 caggccctgg ttgaacagaa gaaagccggc ctgaagctgg gtcagtatct cacccgtcag
      421 ggagtggtca acgaacagca ggtcatcgac atgttgagcc gtcagctcaa tattgccaaa
      481 tatcaccccg atcaatatcc gctggatctg tctctggccc attacattcc cattgacacc
      541 gcacagaaat accaggtagc gcctttgcgg aagaaaggca ggctgctcac cattgccatt
      601 gtcgatcctc tggatatcaa tacgctggat ttcatcgaga tgctgaccaa ttcggaagtg
      661 gagcccgtgg tctgttcgga aagggagctg aaccagatca tcagttcgct ctacgggtca
      721 cagtccggtc tgggcagcat catggaaagc ttggaggtgg ccgcccctga agaaagtcag
      781 gaagaagccg cgccgggcaa agagacggaa gtttccatcg cttccctgca ggacatggcg
      841 ggcgaagcgc cggttgtccg cctggccaat tccatttttg cgcaggccat ccgcgacggc
      901 gccagcgacg ttcacatcag cccgcagcag aacaacgtcc agatgcgttt tcggattgac
      961 ggcaaactga tcgaagttcc atcgccgccc aaggcgcttt ttctgccgat cgttgcccgg
     1021 atcaaaatcc tggccaatat ggacattacc atgtcccgcg ttccccagga cggccgtttc
     1081 acgctgaaaa tgagcagcaa ggaaatcaac gtgagagtgt cctcgattcc gaccatctac
     1141 ggggaaaacc ttgttttgcg aatgctggac atgagcggcg gaatctatac actggaccgc
     1201 ctgggcatga ttccttcaga ccgggaaaag atcgagatga tgagcaagaa gccttacggc
     1261 ctgatcctga gcaccggccc caccggcagc ggcaaaagca caagccttta cgcgattctc
     1321 agcgccatca acaagccgga catcaacatc atcaccctgg aggatcccgt cgaataccgg
     1381 gtcaacaacg tccgccaggt ccagttgaac cggaaagccg gcatgacctt cgcgagcgga
     1441 ttgcgctcca ttctccgcca ggacccggac gtgatcctgg tcggtgaaat ccgcgattcg
     1501 gaaacggcgg cgattgccgt ccaggccgcg cagaccggcc accgcgttct gagcaccgtt
     1561 cataccaacg atgccgcggg cgccgtgacc cgcttcatcg acatgggcat cgagcctttt
     1621 ttgatttctt ccgtgctgct ggtttccttt gcccagcggc tgctgcggac cgtctgtccc
     1681 tactgcaagg aatcctatca gccgccgcaa agcgcgctgg atgcgctggg cattacaaaa
     1741 gagcagggcc aaaacgccaa tttcgcgcgg ggcaggggat gccagcagtg taaaaacacg
     1801 ggctataagg ggcgaaccgg ccttttcgaa gttctggtca acgacgcaat ggttcaggac
     1861 atgatcatga agagatactc caaccagcaa attacaaaag cgacgatcga ggcgggaacg
     1921 ttgagaacct tgattgaaga cgcggcggca aaggttatga acggcatcac cacgccggaa
     1981 gaagcaacat cggcggttat gacctagggt gaaaaaagat ggccaattat gtttattcgg
     2041 caatcaacga aatgggaaag acgataaccg actccataga agcggagtcg gaagaaatag
     2101 tcaacagcat gctggcggcg cgaaacctga tcccggtccg gatcgtgcgg caggacaaaa
     2161 gcggcttcag cctttcgtcg ttgtcttctc tgcgtttcgg ggcttcggtt aaaacaaagg
     2221 atttaattct gttcaccaag cagttccggt ccatgatgca ggccggcgtt cccattatcc
     2281 ggctgatgca ggtgctcgtc agccagacgc aggacaaaac tctcaaaaaa gtggcaacgg
     2341 aaatcagcca ggacatcaaa ggcggtatga cgctttacgc ggctatgaag aagcatccgt
     2401 ccgtcttttc accactatat ctcagcatga tcaacgccgg agagatcagc ggctccgtgc
     2461 cggaaatcct ggagcgcctg atcgatatcg tcgaacatga ggcgaaaatc aaaaaggata
     2521 tcaagtctgc tctgcaatac ccgatcatcg ttgtgattgc cctggtcatc gcgtttgttg
     2581 ttcttctgac ttttgtcatt cccaagtttg ttgctatatt cgccaaagcc ggaattgcgc
     2641 tgccgctgcc gacgaaaatt gccatgatta tgtatcaggg gctggccaat tactggcaca
     2701 tcctgctggg aggcattatc gctctcgttg tctttctgcg gtattacatt aaaaccccgg
     2761 ccgggcgcta cacactggac acttttcttc tgaaaatgcc tctgttcggt tccctgtttc
     2821 agaaggcggc catgtcgcgc tttgcggcga tcttcgcgat catgcaggcc agcggcgttc
     2881 ccgtgatgag aaccatggaa gtggtgtcgg acaccatcgg caacacggca atttcccggg
     2941 aattcgacaa tgtccgggat aaaatacaag aggggcaggg aatttccggg ccgctgcgct
     3001 ccgcgaagca ctttaccccg atggtcgtgg atatggtggc catcggcgaa gaatccggca
     3061 acatcgaaga gatgatgcgg caggtgtctc tccattatga cgacgaagtg caatatgccg
     3121 tcaagggatt ggccgattcg atcggcccga ttttgatggt cggtctcgcc gcggtggtcg
     3181 gcttctttgc cctggcgata ttcatgccca tgtgggattt aactaaaatg gtcaagtgag
     3241 ctccgggtga aagatatcac atcgagcata aaaaaacttt ccgcaggacg ttcttcaccg
     3301 ttccgtgtca gcctctttgt cctgattccc tttatcttca cgggattcac cgttctggcc
     3361 tttcttgttg cctactacgc cggcagagct tcaacgatac agcttttttt acttggcggg
     3421 gcggtcattg cctttaccgc tttttcggcg gttgtcgtaa cgtttgccgt tttgagtccc
     3481 gttcaaaaat ttgtcgatga agtcgaatct tctccgtcgt ttccaaaacc accatccgag
     3541 tcagaaccgg aatcgaaacc taaggttaat gatgaaatcg atcattttga tcacgttttt
     3601 cgtgaagtag ccaatttgat cagcaaagtg gatgcaaggg accggttccc cggaatcgtc
     3661 ggccagagcc gggccatccg gagttgcctg agccaggtca tcaaagtagc gccgacagac
     3721 aatacggtcc ttttgctggg tgaaagcggt gtgggaaaag aacttttcgc ggaagcgatt
     3781 tatcaggaga gcaaccgtca ggggaaaccg tttgtcaagc tcaattgcgt ggccatcgcg
     3841 gccggtctgc tggagagcga gctcttcggt catgaaaaag gatcctttac cggagcttcg
     3901 gccaaaaaga cgggaaaatt cgagctggcc aacgaaggaa cgctttttct ggacgaaatc
     3961 ggcgacatac agttggagac acaggccaag ctgctgcgcg tcctgcagga gcgggagttt
     4021 cagcgcgtcg gcggtaatga aaccatcagg gtcaatgtgc ggtttatcgc cgcttccaac
     4081 aaaaaccttc accagatggt caaagaaggg acttttcgtg aggatctttt ctaccgcttg
     4141 aatgtttttc cgatttatat tccgccgctg cgggatcgcg tggaggacat cccgctcatc
     4201 gcccggcaca tattggatcg tatgcccgaa aaaaaggaac tgtccccgga agcgatgcgg
     4261 gagttgatga catggtcctg gccgggcaat attcgggaat tgaagaatgt gctggagaga
     4321 gcttccgtta tggccgatca ggggagaat
//
LOCUS       DAOPAF010000095         4259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_17040_length_4259_cov_42.948858, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000095 DAOPAF010000000
VERSION     DAOPAF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17040_length_4259_cov_42.948858"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLQ77_07545"
     CDS             complement(<1..431)
                     /locus_tag="PLQ77_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL10568.1"
                     /translation="MNILIVKLSAIGDVIHTLPSLAELRRLYPAAHITWVVEEAAADL
                     VLGHPHLDEVIISRRKSWIKDIKAGKIAATLREVRTFVRRLRSRRYDLVIDFHGLLKS
                     SMMVFLSGGKRKLGYDSWQELSGLFLNEKIPEDMNKHAVDR"
     gene            complement(428..1015)
                     /locus_tag="PLQ77_07550"
     CDS             complement(428..1015)
                     /locus_tag="PLQ77_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPL10569.1"
                     /translation="MKKEAAIFLDRDGTINEEVGYLDSLDKLAIFPSAFEAIRLINQS
                     GLKTIVVTNQAGVARDFFGEDFVETIHAALREALCQRGALIDAFYYCPHHPTEGFPPY
                     RQSCDCRKPAPGLFLRAAMDWNLDLSASWMIGDRYNDIEAAHRAGARGVLVKTGYGAD
                     ALAGAGPDGETLAGKPDLIADDILQAVHLILGSRK"
     gene            complement(1022..2107)
                     /gene="waaF"
                     /locus_tag="PLQ77_07555"
     CDS             complement(1022..2107)
                     /gene="waaF"
                     /locus_tag="PLQ77_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPL10570.1"
                     /translation="MMMLEAKNKLPREGLNKILIRGTNWIGDAVMTLPAVASVRAAYP
                     KAHLSILAKPPVSDIYKMFSAADEILAYSSKFDNAPGVLRLAYSLRSKKFDAAILLQN
                     AIEAAIIALAAGIRVRAGYNSDGRGFLLTHPVRRTTEILRVHQIGYYLEMVKALGCAN
                     VERSPHLETFISAADATDVVRRHLTDMAGPFIGIAPGAAYGPAKRWLPERFAQAGDRL
                     AKELNAQVLLFGGRDDWETTEQVREQAQTNMINLAGRVPLADSVYLMSQCRLFLSNDS
                     GLMHVAGGLNIPTVAIFGSTNPVTTSPAGEKTILVRKETPCSPCLEKTCPTDFRCMTA
                     ITVEDVVAAALTLLNGPANPRRNDQKS"
     gene            complement(2118..3005)
                     /locus_tag="PLQ77_07560"
     CDS             complement(2118..3005)
                     /locus_tag="PLQ77_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL10571.1"
                     /translation="MSLKLKIGLWIFRVIPLAVRRAVFSGLAWLGYRLSVKHRLIVLH
                     NLTRAFPEKSPAEIVRIAKASYRSFGRVVAEFSEITRLNPDNVHQWVRIQGLEHYDEA
                     RRKGKGVLLFSAHFGNWEIGNAAMAIARKPLIFIYRILDSQFLEEAITYVRATCGNIS
                     LDKENAMRPMIRALKKGETINILIDQNVAVYDGIFVDFFGRPACTTSGLALLALHTGA
                     PVLPVFTTRMPDGKYWLEIGAEVAIRNTGNRDADVRESTQTFTGIIEEHIRKYPEQWF
                     WMHQRWKTKKCQARMVNSE"
     gene            complement(3021..4124)
                     /gene="lpxK"
                     /locus_tag="PLQ77_07565"
     CDS             complement(3021..4124)
                     /gene="lpxK"
                     /locus_tag="PLQ77_07565"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPL10572.1"
                     /translation="MIDWPGIWNENDSSRRISPTRAILYLGSLVYRLIVSSRNRLYDR
                     QILKFVRLARPVISVGNLTVGGTGKTPCVIGLAKMLARRGYRPVVLSRGYGGKNQASV
                     NIVSDGKNIMLGAEEAGDEPLLIARSLPGIPVLTGAKRALTGQAAIERFNADVLICDD
                     AFQHRQIFRDIDIVLLDADRPLGNDRLLPRGELREPAGSLRRADCIVLTRADKAGTPP
                     DEIARITRERSIPVFRSVHRLREMVRPREHLTLPPENFRGKSICAFCGIARPASFKKS
                     LLDAGMHILSFIDFPDHYAYKRDDLEAIQKHFSTQNADAWITTEKDAMRLEESPDFLN
                     QLFIARMEMEIQPSTPSFDDFILRRLKDLILKT"
BASE COUNT          872 a         1183 c         1233 g          971 t
ORIGIN      
        1 tagcggtcca ccgcgtgctt gttcatgtct tccggaattt tttcattgag aaataatccg
       61 ctcagttcct gccaggaatc atatcccagt tttctcttcc ctccacttaa aaaaaccatc
      121 atcgaacttt ttaaaagacc gtggaaatca atcacgagat cgtagcggcg gctcctgagc
      181 cggcggacaa acgttctcac ttcccgcaat gtcgccgcga ttttccccgc cttgatatct
      241 tttatccagc ttttgcgccg ggaaatgata acctcgtcca gatgcggatg gccgaggacc
      301 agatccgccg cagcttcttc tacaacccag gtgatgtgtg ccgcgggata gagacggcgc
      361 agctccgcaa gggacgggag cgtgtgaatc acatctccga tggcgctcag tttgacgatc
      421 agaatattca tttacggctc cccaggatga gatggacggc ctgaaggata tcgtccgcaa
      481 tgaggtccgg ctttcccgca agcgtttctc catcggggcc cgcaccggca agggcatccg
      541 cgccgtagcc cgttttgacc agaacgcctc tggcgccggc ccggtgagca gcctcgatat
      601 cgttgtaccg gtctccgatc atccaggatg cggataaatc cagattccaa tccatggcgg
      661 cccgcagaaa caggccggga gccggcttgc ggcagtcgca gctttgccga taggggggaa
      721 agccctcagt cggatgatgc gggcagtaat aaaacgcatc gatcagcgca cccctttgac
      781 acagcgcctc ccgcagggct gcgtgaatgg tttccacgaa gtcttcaccg aaaaaatccc
      841 gggcgacgcc ggcctggttg gtgacgacaa tcgtcttgag gccgctttga ttgatgaggc
      901 gaatcgcttc aaaagcggat gggaaaatgg ccagcttgtc caaagagtcg agatagccga
      961 cttcttcgtt gatggtcccg tcccgatcca gaaatatggc tgcttctttt ttcatttcgc
     1021 gtcaactctt ttggtcattc cggcgcgggt tggccgggcc gttcagcagg gtcagcgccg
     1081 ccgcgacgac gtcttcgacg gtgatcgcgg tcatgcagcg gaaatccgtc ggacaggttt
     1141 tttccaaaca gggactgcac ggcgtttcct ttcgcaccag gatcgttttt tctcccgcgg
     1201 gcgaggtggt cacgggattg gtggagccga aaatggcgac cgtcggaata ttcaagccgc
     1261 ctgctacgtg catgaggccc gaatcattgc tgagaaacag ccggcattgc gacatgagat
     1321 aaaccgagtc cgcaagcggt acgcggccag ccagattaat catattggtt tgcgcttgtt
     1381 cgcgcacctg ttcagtcgtt tcccaatcgt ccctgcctcc gaagagaaga acctgcgcgt
     1441 tcaattcttt tgccaggcgg tctcccgcct gcgcaaatct ttccggaagc caccgttttg
     1501 ccggtccata ggcggcgccg ggcgcaatgc cgatgaaggg tcctgccata tccgtaaggt
     1561 gccgccggac gacatccgta gcgtccgccg cggaaatgaa agtttccagg tgcggcgagc
     1621 gttccacgtt cgcgcagccc agcgctttga ccatttccag atagtagccg atctgatgga
     1681 cgcgaaggat ttccgttgta cgccggacgg gatgcgtcag tagaaagccg cggccgtccg
     1741 agttgtagcc ggcacgcacg cgaatgccgg cggccagtgc gatgatggcg gcttcgatgg
     1801 cgttttgcag caaaatggct gcgtcgaact ttttagatcg cagcgaatac gccaggcgaa
     1861 ggacacccgg cgcattgtcg aatttgcttg aataggcaag aatttcatcg gcggcggaaa
     1921 acattttgta gatgtcggag acgggcggct tggccagaat ggacagatgc gctttcggat
     1981 aggccgcgcg cacggacgcg acggcgggca gcgtcatcac ggcgtcgccg atccagttgg
     2041 tcccgcggat caggattttg ttcagtccct cccgcggcag tttatttttt gcttccagca
     2101 tcatcatcca ccatccacta ttcactattc accattctcg cctggcattt cttcgtcttc
     2161 caccgctggt gcatccagaa ccattgttcg ggatatttgc ggatgtgctc ttcaatgatt
     2221 ccggtgaagg tctgggtgct ttcccgcaca tcggcatcgc ggtttcccgt gttgcggatg
     2281 gcgacttccg ccccgatctc caaccaatat tttccgtcgg gcatgcgggt ggtaaaaacc
     2341 ggcagaaccg gcgcaccggt atggagcgcc agcagggcaa ggccggaggt cgtgcaggcc
     2401 ggccgcccga aaaaatccac aaagattcca tcgtaaaccg ccacgttctg gtcaatcaga
     2461 atattgatgg tttctccctt tttcagggcg cggatcatcg gccgcatggc gttttccttg
     2521 tccagggaaa tattgccgca ggtcgcgcga acataggtga ttgcctcttc aagaaattga
     2581 ctgtccagaa tgcggtaaat gaagatcaga ggttttctgg cgattgccat tgcggcgttg
     2641 ccgatttccc agttgccgaa gtgcgcgctg aacaggagaa cgccttttcc ttttcggcgg
     2701 gcctcgtcgt aatgttcgag gccctgaatg cggacccatt gatgcacatt gtccggattc
     2761 agacgtgtga tttctgaaaa ttccgccacg acccggccaa agcttcggta ggaagctttg
     2821 gcaatgcgca caatttcggc gggggatttt tcaggaaagg cacgggtcag attgtgcaga
     2881 acaatcagcc ggtgtttgac cgacagccgg taccccagcc aggcgaggcc ggagaagacg
     2941 gcccgtctga ccgcaagggg tatcacgcga aagatccaca gaccgatttt cagtttaaga
     3001 ctcataatgg aaaaatagtt ttaagtttta agtattaagt ctttcagccg tcgcagaata
     3061 aagtcgtcaa aggacggtgt ggacggttga atttccattt ccatgcgcgc gatgaaaagc
     3121 tggtttaaaa aatccggact ttcctccagg cgcattgcgt ctttttccgt cgttatccag
     3181 gcgtccgcgt tttgcgttga aaaatgcttt tgaatcgcct ccaggtcatc gcgtttgtaa
     3241 gcgtaatgat ccggaaagtc aataaatgaa aggatgtgca ttcctgcgtc gagcagtgat
     3301 tttttaaagg aggcggggcg ggcgattccg cagaaggcgc agatactttt gccgcgaaag
     3361 ttttccggcg gcagggtaag atgctcccgc ggccggacca tttcccgaag ccggtgaacg
     3421 gagcggaaga cggggatgct ccgttcgcgg gtgatccgcg cgatttcatc gggaggggtt
     3481 ccggctttgt cggcgcgcgt cagtacgatg cagtccgcgc ggcgaaggct tcccgcgggt
     3541 tcacgaagtt cgccgcgcgg cagaagtcgg tcgttgccca gcggcctatc cgcgtccagc
     3601 aaaacgatat cgatgtcccg gaaaatctga cggtgctgaa acgcgtcgtc gcagatcagc
     3661 acgtcggcgt tgaatcgctc aatggccgcc tgtccggtga gtgcccgctt ggcgccggtc
     3721 aggaccggaa tgccggggag cgaccgggcg atcagcagcg gttcgtcgcc ggcttcttcc
     3781 gcgccaagca tgatgttttt tccgtcggaa acgatattga cggacgcctg atttttcccg
     3841 ccgtagccgc ggctgaggac aaccggccgg tatccccgac gcgccagcat ttttgccagc
     3901 ccgatcacgc agggggtttt gccggttccg ccgaccgtca gatttcccac gctgatcacg
     3961 gggcgggcca ggcggacgaa cttcagaatt tgccggtcgt aaagacggtt tcgggaagag
     4021 acaatcagcc ggtagaccag ggagcccaga taaagaatgg cccgggtcgg actgatgcgg
     4081 cgagacgaat cgttctcatt ccatatccct ggccagtcga tcattgccgt ttccctcgtc
     4141 cttgaactgc aggttgtata atcggtagta ttcgcctttt ttctccatca gggcgtcgtg
     4201 gtttccttcc tccacgattt ctccgttgac cagcgcgatg atccggtcgg cgttgcgga
//
LOCUS       DAOPAF010000096         4229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_17190_length_4229_cov_49.147341, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000096 DAOPAF010000000
VERSION     DAOPAF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17190_length_4229_cov_49.147341"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLQ77_07570"
     CDS             complement(<1..305)
                     /locus_tag="PLQ77_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HPL10573.1"
                     /translation="MNKIYRLIWSRSREMWIAVSEKVAAGWFRRPLTVGALVLSALLA
                     AGGTAKALDPHALPTGGQVVAGTAAISQAGSAMTINQHTDKMIANWNTFNIGQNASVA
                     "
     gene            complement(468..1202)
                     /locus_tag="PLQ77_07575"
     CDS             complement(468..1202)
                     /locus_tag="PLQ77_07575"
                     /inference="COORDINATES: protein motif:HMM:NF018928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HPL10574.1"
                     /translation="MTEKGNLPQFYILPVVLNRDVHAALTISESPARFTFAARAQTVV
                     IASVEFFDACRQFPVIFTRTDDGRIVPLALMGLENDENLFVDDAGNWLGAYIPAYIRR
                     YPFITTDGADGQAVVCFDEAFDGFNREGGTPLFENGGPSPKMKEVIGFLDDYFIRMKE
                     TEQLGAFLAENGLLRQIDAQANLDDGRSYALNGMLVVDEPRLAQLPDADIVQLFRSGL
                     LGLIQAHLISLRNLGVLIDRKAGKTS"
     gene            complement(1239..2240)
                     /locus_tag="PLQ77_07580"
     CDS             complement(1239..2240)
                     /locus_tag="PLQ77_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HPL10575.1"
                     /translation="MGQIALYDPLGLGDQLSASVTGAERMLSGRAAYAIPLGSSGLTA
                     GAYYNYLQYNLGEELKDLNAKGWAHTLGASLTYPILRTRGASVWAGLGFEYLMLDDEA
                     NNVQTRDRKLPVGNLNLTGSFYDSFGGGGLTNANLAFYGGSVDLSGLQANKIADEAGP
                     QTGGGFSRGAYSLARLQRITRSLSLYGSVRGQLASGNLDSSQKFILGGPTGVRSYPVG
                     EAPGDEGHAGTLEARLDLPFMPAWATTQVVGFFDAGWVKLHRTLYTGALTNATGRNDY
                     TLSGGGIGINVGKPGLYSLRASYAHKIGLNEGRSPTGKDCDNRSDQGRLWLQLVVWI"
     gene            complement(2247..2969)
                     /locus_tag="PLQ77_07585"
     CDS             complement(2247..2969)
                     /locus_tag="PLQ77_07585"
                     /inference="COORDINATES: protein motif:HMM:NF020068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPL10576.1"
                     /translation="MNIHLKCSLFVILSLFIFCATAFAQPDAGTLLQEQRQLTPSLPD
                     RLPTEEKREIVKPPLADTGIKVLVKGFRFTGHYETMTTEGELQSLVSGSVGKELRFAE
                     LQQLAAQVTNYLREKKGFLLARAYLPKQDITEGIIEIAIMDGRIDGNVRINLKEPSRI
                     RPGLLQGMADRAVPGNAPVRMEQVERTVLLMNDLPGINAQASLEPGSAPGTTRLVINA
                     AEGRLFHGVLSADNYGDRYTGA"
     gene            complement(3098..4162)
                     /locus_tag="PLQ77_07590"
     CDS             complement(3098..4162)
                     /locus_tag="PLQ77_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10577.1"
                     /translation="MNDPSNTYQELVEENSLLKQKVQELEVIAGERRKAERGLRDTEN
                     KFKILTEKMSDIAWIADMNLKTLYVTPSIKTVLGFSQEERILQDIGKQLTPDSLSMAM
                     DVMAREIALEEEGTSDPARTLHLELEFVHKNGATRWMDVVINGTRNDQGKLNGIHGVA
                     RDVTDRKQAEASLKKAHEQLENRIQERTEELAKAVQHLKENELLLAEESRRFQETNTA
                     LKVLLRQREDDQHDLERKVLTNVKNLVLPYVEKLKSTHLNPVQANYADIIFANLQNIV
                     SPFLRNLTSTYMDFTPREIEVANLVREGKSAKEIALLLNCSLRSVEFHKENIRRKLGL
                     IHKKTNLRTHLLSLNTTLTT"
BASE COUNT          867 a         1139 c         1187 g         1036 t
ORIGIN      
        1 gccacggagg cgttctggcc gatgttgaag gtgttccagt tggcgatcat cttatcggta
       61 tgctggttga tcgtcatggc gctgcctgcc tggctgatgg cggctgtacc tgcgacgacc
      121 tggccgcctg tgggaagggc atggggatcg agggcctttg ccgttcctcc cgcggccagc
      181 agagccgaca ataccagagc ccccaccgtc agaggacgcc gaaaccaccc ggccgcaacc
      241 ttctccgaaa ccgctatcca catctcccgc gaacgggacc agatcagccg ataaatcttg
      301 ttcatgaagt catctccaat ttttcaatgg gaccggattg cccatcatca attttcgtca
      361 ttccgtcgaa agacggaatc cagcctttgt tttcctggat gccggttttc accggcatta
      421 ccctcttgct ctactcttca ggcatgacgg cgttactctg ttcaccatta tgacgttttt
      481 cccgccttcc ggtcgatcag gacgcccagg ttgcgaagcg agatcaggtg ggcctgaatc
      541 agcccgagca acccgctgcg gaaaagctgg acaatgtccg cgtccggcag ctgggccagc
      601 cggggttcat cgacaaccag cattccgttc aaggcgtagc tccggccgtc gtccagattg
      661 gcctgggcgt cgatctggcg gagcagaccg ttttccgcca gaaatgcacc caactgctcc
      721 gtttctttca tcctgatgaa gtaatcatcg agaaacccga tgacctcctt cattttcggg
      781 cttggtccgc cgttttcaaa caggggcgtt cctccctccc ggttgaagcc gtcaaaggct
      841 tcgtcaaagc agacaaccgc ttgaccgtcg gcgccgtcgg tggtgatgaa agggtagcgc
      901 cggatgtagg ccgggatgta ggcgcccagc cagttgcccg cgtcatccac aaagaggttt
      961 tcgtcgtttt ccagacccat gagggccaga ggaacgattc gtccatcgtc ggtccgggta
     1021 aaaatgacgg gaaattgacg gcaggcatcg aaaaactcaa cggaggcgat gaccacagtc
     1081 tgggctcggg cggcaaaggt gaatcgcgcc gggctttcgg aaattgtcag ggcggcatgg
     1141 acgtcgcgat tgagaacaac cggcaggata taaaattgag gaaggttgcc tttttcagtc
     1201 atggtaggac tccctggaaa atggcggttg aggttgtgtt aaatccaaac gacaagctgg
     1261 agccacaggc ggccctgatc gctgcggtta tcgcagtctt tgccggttgg actgcggccc
     1321 tcgttgaggc cgatcttgtg ggcataggag gcccgcaggc tgtaaagtcc gggcttgccg
     1381 acattgatgc cgatgccgcc gcccgaaagc gtatagtcgt ttcttcccgt ggcgttggtc
     1441 aacgcaccgg tgtaaagcgt tctgtgcagc ttcacccagc ctgcatcgaa gaatcccacc
     1501 acctgcgttg tcgcccaggc gggcataaag ggaagatcca gacgggcttc cagtgttccg
     1561 gcgtggcctt cgtcaccggg cgcttcgccg acagggtagg atctgacgcc ggtgggtccc
     1621 cccagaatga acttctgtga ggaatccagg ttgccggaag ccaactgccc ccttaccgat
     1681 ccgtacaggg acagggagcg ggtgatgcgc tgcaggcgcg ccagggaata ggcgccccgt
     1741 gagaagcctc cacccgtctg cggtcccgct tcatcggcaa ttttgttggc ctgcaaaccg
     1801 gagagatcca cgctgccccc gtaaaaagca aggttggcgt tggtcagtcc gccgccgccg
     1861 aagctgtcgt aaaagctgcc cgtgagattg agattgccga cgggaagttt gcggtcgcgg
     1921 gtttggacgt tgttggcctc gtcgtcgagc atcagatatt caaatcccaa acccgcccaa
     1981 acgctggcgc cgcgggttcg caaaatcggg taggtgaggc tggcgcccag cgtgtgtgcc
     2041 cagcctttgg cgttcaggtc tttgagctct tctcccagat tgtattgcag gtagttatag
     2101 taagcgccgg cggtcagtcc cgatgaccct aggggaatgg cgtaggcggc ccttcccgac
     2161 agcatgcgtt ccgcgccggt gacggaagcg gaaagctggt ctcccaatcc cagggggtcg
     2221 taaagggcaa tctgccccat cccccgctag gctcccgtgt agcggtcgcc gtagttgtcc
     2281 gcggaaagaa cgccatggaa aagccgccct tcggcggcgt tgatgaccag tctggtggtc
     2341 cccggcgcgc tgcccggctc cagtgaggcc tgggcgttga tgccgggaag gtcgttcatg
     2401 aggagaacgg ttcgttcaac ctgctccatg cgaaccggag cgttcccggg gacggcccgg
     2461 tcggccatgc cctgcagaag cccgggacgg atgcggctgg gctccttgag gttgatgcgg
     2521 acgtttccgt cgatccgccc gtccatgatg gcgatctcaa tgatgccttc ggtaatgtct
     2581 tgtttgggaa ggtaggctcg ggccagaagg aagcctttct tttcccgcag ataattcgtt
     2641 acctgggccg caagctgctg aagttcggcg aaacggagtt ctttgccgac gctgccggaa
     2701 accagtgact gtaattcccc ctcagtggtc attgtttcat agtgcccggt aaagcgaaac
     2761 cccttgacga gcaccttgat gcccgtgtcg gcaagcggag gcttgacgat ttctcttttt
     2821 tcttcggtgg gcaggcgatc gggcagggaa ggtgtcagtt gccgctgttc ctgcaaaagt
     2881 gtgccagcat cgggttgagc aaaagccgtg gcgcaaaaaa tgaaaagaga gaggatgaca
     2941 aacaacgaac atttaagatg tatgttcaaa agtttatctc ctttaaattg acaccgttga
     3001 acctctgatt cttgcaattg taaaggagta tatattgata aattcctgtt tatcaatacc
     3061 aatacattgc tgagggaata ccaggtaatt actagtatta tgtcgtgaga gttgtgttca
     3121 gggacagcaa gtgggttctc agatttgttt tcttgtggat aaggccgagc tttcgccgga
     3181 tattttcctt gtgaaactca accgatctca gggaacagtt cagcagcaac gcaatttcct
     3241 tggcggattt tccttcgcgg accagattgg ccacttcaat ttcccgggga gtgaaatcca
     3301 tataagtgga tgtcagattt ctcagaaaag gggaaacgat gttttgcaga ttggcgaaaa
     3361 taatatcagc gtaattggcc tgaaccgggt tcaaatgagt ggatttgagt ttttccacat
     3421 aaggcagcac cagatttttc acattggtca ggacttttct ttccagatca tgctgatcgt
     3481 cttcccgctg acggagcaag acttttaaag cggtattggt ttcctgaaac ctgcgggact
     3541 cttccgccag aagaagttca ttttccttca agtgctgaac cgcttttgcc agctcctccg
     3601 tgcgctcctg aatacgattt tccagctgtt catgagcttt tttcagagac gcctcggctt
     3661 gtttgcggtc ggtaacatct cttgccacgc cgtgaattcc gtttagcttg ccctggtcat
     3721 tccttgtccc attgataacg acatccatcc agcgggtggc tccgttttta tgaacaaatt
     3781 ccagttccag atgcagcgtt cttgcgggat ctgaagtgcc ttcttcttcc agggcaatct
     3841 ctctggccat cacatccatc gccatggaca aggaatcggg cgtcaattgc ttcccgatgt
     3901 cctggagaat tctttcttcc tggctgaatc cgagcacggt tttgatggaa ggggtgacat
     3961 agagagtttt caggttcata tccgctatcc aggcgatatc cgacattttt tcagtaagaa
     4021 tcttgaattt gttttcggtg tcgcgaagcc ccctttccgc ttttcttcgt tctcccgcta
     4081 taacttccag ctcctgaact ttttgcttta acagggaatt ttcttcgacc agttcctgat
     4141 aggtgttgga tggatcattc atctttgaat gtactcctgc acgagacgga tcatcatatc
     4201 cgcgttcttg cgcgttccgg cgacgattt
//
LOCUS       DAOPAF010000097         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_17210_length_4225_cov_50.354676, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000097 DAOPAF010000000
VERSION     DAOPAF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17210_length_4225_cov_50.354676"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..657)
                     /locus_tag="PLQ77_07595"
     CDS             complement(13..657)
                     /locus_tag="PLQ77_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPL10578.1"
                     /translation="MMALHILGISGSPVKKGNVGTFLNLALECLPQKETKREVIHLSG
                     LKIQDCIHCNFCLTKQMPGKYCSLKDDAQVVFEKAEASDILVLASPVYFMRSSARMAA
                     LLDRFRVFVFGNLTGGKMKNKIGVSMAVSWLRNGGLETTHLAHILEFLTLEMIPASVH
                     QGISPLGASACASSYGSGKFDPNVKLGIENDEAGLQSAKLILRRAVELARLVSK"
     gene            complement(670..1188)
                     /locus_tag="PLQ77_07600"
     CDS             complement(670..1188)
                     /locus_tag="PLQ77_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPL10579.1"
                     /translation="MKKILLLIVLLLTGCAGLPPGVTPVEGFQLERYLGKWYEIARLD
                     HSFERGLSSVSAEYSLRSDGGVQVVNRGYAAGDGRWKEAEGRAYFVNRSDQGFLKVSF
                     FGPFYGSYVIMELDRKNYGWALVCGPDRSYLWILARSGTLEEETRNRLIAKAAALGFE
                     TDRLIFVRHNQK"
     gene            1260..>1636
                     /locus_tag="PLQ77_07605"
     CDS             1260..>1636
                     /locus_tag="PLQ77_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPL10580.1"
                     /translation="MRLDEYFENARGVGVLSTSDAGGKVNAAIYGRPHFMDDRTVAFI
                     ARNRLTRANLLTNPSAVYLFKEDGSYEGKRLYLTKIKEEKDSPLIGEIRRRKHREVEG
                     KDPAEEKFLIYFTIDKVLPLIGDG"
     assembly_gap    1637..1736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1774..2679
                     /locus_tag="PLQ77_07610"
     CDS             1774..2679
                     /locus_tag="PLQ77_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPL10581.1"
                     /translation="MKVFMTGGTGFVGTYLTKKLIDKGHNVCVLTQSLTGRELKIPGL
                     TYLVGDPNVRGKWQDAVRDHEIIINLAGASIFSRWTDEQKKILRDSRLNTTRHLVEAL
                     PADASRVLFFSTSAVGYYGFHEDEELTESSPAGGDFLARLASDWEAEAMRAKEKGARV
                     VITRFGIVLGPNGGALGQMIPLFKFFLGGPLGSGRQWFSWVHLQDLTAAFLFLIGHPE
                     ISGAVNLCAPQPVRNRELGNAIGRVLHRPSFLPAPGFMIEIILGEFGSVLLKGQRVIP
                     RRLLDAGFRFSYPEIDEALHSIIKP"
     gene            2784..>4225
                     /locus_tag="PLQ77_07615"
     CDS             2784..>4225
                     /locus_tag="PLQ77_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPL10582.1"
                     /translation="MKFLSAIISTISGSKIVKANIGLLAKYLLVIIVLITAYSIVFHY
                     LMALEGQKHSWMTGFYWTMVVMSTLGFGDITFTSDIGRAFSVLVLLSGVILLLILLPF
                     VFIKFFFAPWMEAQARSRAPRELPPDTRNHVIITHYDPVTADLIEKLVSYKKDYVVVV
                     DELQRAIQLYDKNIRVAVGDIDDPKTYFRLRAQNAALVVATNSDQMNTNITLTLREDF
                     EHLPIVTTSESPHSVDILQLAGSSRVLQLHDLLGGSLAAWTIAGDCKSNMIGRYDQLV
                     IAQAPVIGTPLVGKTLAESGIRETFGLTVVGIWERGTFMIPGPETTIDRTSVLVFAGT
                     EAAVAAYDEVYSFYQICKVAGDPVIIVGGGRVGHAIALRFQKADIPYLIIERAKKRSE
                     EDGHFVFGDAADIHTLQKAWFEKAPAALITSHDDATNIYLTKYLRSLRPDMQILSRAS
                     EDRNVSTLHRAGADFVMSYASLGANAIFNFL"
BASE COUNT          876 a         1262 c         1110 g          877 t
ORIGIN      
        1 attgatcctg gattatttgc tcaccagccg ggccagttcg acggcccgcc gcagaatcag
       61 ctttgccgat tgcaggcctg cctcgtcgtt ttcgatgcca agcttgacgt tcggatcgaa
      121 tttgccggaa ccgtaggagc ttgcgcaggc cgacgcgccc agagggctta tgccctgatg
      181 gacgctggcc ggtatcatct ccagcgtcag aaactccagg atgtgcgcga ggtgcgtggt
      241 ctccaggccg ccgttgcgca gccaggagac ggccatgctc acgccgattt tgtttttcat
      301 ttttccgccc gtcaggtttc cgaagacaaa cacgcggaac cggtccagca gcgcggccat
      361 ccgcgcggac gaccgcatga aatacacggg gctcgccagc acgaggatgt ccgacgcttc
      421 ggctttttca aaaacaacct gtgcatcatc tttaagggaa caatatttac ccggcatttg
      481 tttggtcagg cagaaattac agtggatgca atcctgaatt ttcaaaccgg agagatgaat
      541 cacttcccgt tttgtttctt tttggggcaa gcattccagg gcgaggttca aaaaggttcc
      601 cacattgcct ttttttaccg gggagccgga gattcctaaa atatgaaggg ccatcatgct
      661 tctcctcgtt tatttttggt tgtgccgcac aaaaatcaac cgatccgtct caaaccccag
      721 cgccgcggct ttggcaatca aacgattcct cgtttcttcc tccagcgtcc cgcttcgcgc
      781 aagaatccac agataggagc ggtcggggcc gcataccagc gcccagccgt agttcttcct
      841 atccaattcc ataatcacgt aagacccgta aaacgggcca aagaaggata ctttcaaaaa
      901 cccctgatcg gaacggttca cgaaatacgc ccggccctcg gcttctttcc acctgccatc
      961 tcctgccgca tagccccggt tcaccacctg cacgccgccg tcggaccgca ggctgtactc
     1021 tgccgaaacg gatgaaagcc ccctttcaaa ggaatgatcc agccgcgcga tttcatacca
     1081 cttgcccagg tatctctcca actggaaacc ttccacaggc gtcacgcccg ggggcagacc
     1141 ggcgcacccg gtcaagagca ggacaataag caaaagtatt tttttcattt tcgcgccttt
     1201 acattccgct tcgaatgcct taatttactt taaaaccgga taaaatcaaa ggaggaatca
     1261 tgcggcttga cgaatatttt gaaaacgccc ggggggtcgg cgttctttcc acttccgatg
     1321 ccgggggaaa agtgaacgcg gccatttacg ggcgtcctca cttcatggat gaccggaccg
     1381 tggcttttat cgcccgcaac cggctcaccc gcgccaacct gctgaccaat ccttccgcgg
     1441 tctatctttt caaggaggat ggctcctacg aaggcaaacg gctgtatctg accaaaatca
     1501 aggaagaaaa agactcgccg ctcatcggcg aaatccgcag gagaaaacac agggaagtgg
     1561 aaggcaagga tccagccgaa gagaaattcc tgatttactt taccatcgac aaggtgctgc
     1621 ctttgattgg cgacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat
     1741 tcgacgggtg atcttaaaac ggagggttga accatgaaag ttttcatgac ggggggaacc
     1801 ggttttgtcg gcacctatct gacgaaaaag ctcatagaca aggggcacaa tgtctgcgtc
     1861 cttacgcagt cgctcaccgg ccgggaattg aaaattccgg gcctaaccta cctggtcggc
     1921 gatcccaacg ttcggggcaa atggcaggac gccgtaaggg atcatgagat catcatcaat
     1981 ctggccggcg cctccatctt cagccgctgg acggacgagc agaaaaaaat tctccgcgac
     2041 agccgcctca acaccacccg gcatctggtg gaagcgctcc ccgcggacgc ctcccgcgtt
     2101 ttgtttttca gcacttcggc ggtcgggtat tacgggtttc atgaagacga ggagctcacc
     2161 gaatcctccc cggcgggcgg cgactttctg gcccgtctgg cttccgactg ggaagccgag
     2221 gccatgcgcg ccaaagaaaa aggcgcgcgc gtggtcatca cccgcttcgg catcgtgctc
     2281 ggccccaacg gtggcgcgct gggtcagatg atccctctgt tcaagttctt cctgggaggc
     2341 ccgctgggtt cgggacgcca gtggttttca tgggtgcacc tgcaggacct cacggccgca
     2401 tttctctttc tcattgggca tccggaaatc tccggcgcgg tgaacctgtg cgcgccccag
     2461 cccgtaagaa accgggaact gggaaacgcg atcggccgcg ttctgcaccg cccctccttc
     2521 ctgcccgccc ccggcttcat gattgaaatt attctgggcg agttcggctc ggtgctgctc
     2581 aaaggccagc gggtcatccc ccgccgcctg ctggatgcag ggtttcgctt ttcctatccg
     2641 gagatcgacg aagcgctgca cagcatcatc aaaccataga tgatccgccg ctcggcgctt
     2701 cagaagcggc ctgattttga ttgacaggcc ccgcggcgct tcatatactg cagccatcag
     2761 cgaagaattc aaaggcgtct ttcatgaaat ttttgtcggc tattatcagc accatctccg
     2821 gaagcaaaat cgtcaaggcc aatatcggcc ttttggcaaa atacctcctg gtcatcatcg
     2881 tgctgattac cgcctacagc attgtctttc actatctcat ggcgctggag ggccagaagc
     2941 attcctggat gaccggcttc tactggacga tggtggtcat gtccacgctg gggttcggag
     3001 acatcacctt caccagcgac atcggccggg ccttttccgt cctggtgctt ctttccggcg
     3061 tgattctgct gctgatcctt cttccctttg tcttcatcaa gttctttttc gccccctgga
     3121 tggaggcgca ggcgcgaagc cgcgcacccc gggagcttcc gccggatacc cgcaaccacg
     3181 tgatcatcac gcattatgac ccggtgaccg ccgacctgat cgagaagctg gtgagctaca
     3241 agaaggacta tgtcgtcgtc gtggatgaac tgcagcgggc gattcagctc tacgacaaaa
     3301 acatccgcgt cgccgtcggc gacatcgacg accccaaaac ctactttcgg ctgcgcgccc
     3361 agaacgccgc gctggtcgtg gccaccaaca gcgaccagat gaacaccaac atcaccctca
     3421 cgctgcggga agacttcgag catctgccga ttgtcaccac gtcggagtct ccccattccg
     3481 tggacatcct tcaactggcg ggaagttcgc gcgttctgca gcttcacgat ctgctgggag
     3541 gatcgctggc ggcctggacc atcgcgggtg actgcaaatc caacatgatc ggccgctacg
     3601 atcagctcgt gatcgcgcag gcgccggtga tcggcacgcc gctggtcggc aaaaccctgg
     3661 ccgaaagcgg aatccgcgaa accttcggcc tgacggtggt gggcatctgg gagcggggca
     3721 ctttcatgat tcccggaccg gagacgacga tcgaccgcac cagcgttctg gtgttcgccg
     3781 gcacggaagc cgccgtcgcg gcctacgatg aggtgtattc cttctaccag atctgcaagg
     3841 tggcgggcga cccggtcatc attgtcggcg gaggacgcgt cggccatgcc atcgccctgc
     3901 gctttcaaaa ggcggacatc ccctacctga ttatcgaacg ggccaaaaag cgaagcgagg
     3961 aggacgggca ttttgttttc ggggacgcgg cggacattca caccctgcag aaggcctggt
     4021 ttgaaaaagc ccccgcggcg ctcatcactt cgcacgacga cgccaccaac atttatctca
     4081 ccaagtacct gagaagcctt cggccggaca tgcagatcct gagccgcgcc agcgaggacc
     4141 gcaacgtctc cacgctgcac cgcgcagggg cggacttcgt catgtcctac gcctcgctcg
     4201 gcgccaacgc catcttcaat tttct
//
LOCUS       DAOPAF010000098         4000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_18375_length_4000_cov_61.290241, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000098 DAOPAF010000000
VERSION     DAOPAF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18375_length_4000_cov_61.290241"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLQ77_07620"
     CDS             complement(<1..652)
                     /locus_tag="PLQ77_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10583.1"
                     /translation="MILPLCSSALAGGMFGPLQTMSKKTGGVNTAVGCRYAEDTLKGD
                     MDYGIRQHQVYSQAAYGASGRWEIYGRIGISDLQISDAFRSSSALTTVSRDSFEENWK
                     FFGTLGAKAYYPVHSVFGVGAFVQGSCNFSNFTDEVSGTSGGIPFVTDIKVKYLWDVN
                     FGIGLQATVPYGIQLYAGPFVYYTEADAYVPAAVPGIQSGAEKTSLKNASAVGGFFG"
     gene            complement(709..1596)
                     /locus_tag="PLQ77_07625"
     CDS             complement(709..1596)
                     /locus_tag="PLQ77_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HPL10584.1"
                     /translation="MSDGQGVVGDGRWVMGNGKSMDYALAKAPSAGADATVRGEEALP
                     GRADDAFQDEYKDDENQDYDDEPFAEERVTIADPIEPFNRAMHRFNDRLYFWLLKPVA
                     RGYKAVVPEPARISVKNFYYHLLFPIRFVNCLLQADLTGAGSEAGRFAINTVWGIGGL
                     LDPSSNRGIELQKQNTDFGQTLGLYGVGHGFYIVWPFLGPSSPRDSVDIAGEYVLDPL
                     SYFTPWYTSLGKRPFKMINNASLSLGDYEAVIEAAIDPYIAIRNGYIQYRMKEVQTRR
                     ARSLFFRNWKSEATRAPAN"
     gene            complement(1712..2320)
                     /locus_tag="PLQ77_07630"
     CDS             complement(1712..2320)
                     /locus_tag="PLQ77_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPL10585.1"
                     /translation="MENRILKKRLAGCMLVMMLLMSVPAYAGAPMSAVESTVNRVLDV
                     LRDPKMKGPAAKSLKTEKLRVIYKDMFDEMEFSRRTLTRNWNKFTPEQRREFVDLFEQ
                     VLEKTYLDRILEYSNEKIVFYKESMIADDKAEIQSRVITSSKEVPIYYRMILKNGKWK
                     VYDVVVENVSLVQNYRTQFNEILAGGTPEQLLETLRKKVKTH"
     gene            complement(2321..2812)
                     /gene="mlaD"
                     /locus_tag="PLQ77_07635"
     CDS             complement(2321..2812)
                     /gene="mlaD"
                     /locus_tag="PLQ77_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HPL10586.1"
                     /translation="MKKYKMETMVGIFVVCGLICVGYMTVKLGKLNWFSGDTYPLIAK
                     FTTVTGLKAGNPVNILGIEVGRVERISMDQEDLRAVVEIRIRKDIKIYDDAIASIKTE
                     GLIGDKYLSIDPGGGGDLLEPNGVITDTQAALDIESLISKYAFGDIKKDQGKNQNNEK
                     EKK"
     gene            complement(2817..>4000)
                     /locus_tag="PLQ77_07640"
     CDS             complement(2817..>4000)
                     /locus_tag="PLQ77_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL10587.1"
                     /translation="LDRVNLQIYEGEVTTIIGLSGSGKSVLLKHIIGLLQPDDGVILF
                     RGKPVAEMSRSDLAGSFGRISYMFQGNALFDSMTVYENIALPLIETTNLKKTEIHRKV
                     LTRMKQTELTEAASKYPSELSGGMQKRAALARALITDPGIVLFDEPTTGQDPVRKNAI
                     LSMIAQNQRKLNFTAVLVSHEIPDVYFISNRILALYDHRIVFQGTPEELENFNHPFMD
                     EVINSLEGLQKELTGLYSKRQFELLNHLQLKRSKGGETFCLVIFSLRELDAIVSQIGH
                     DAAQEILRNMGLFIDRHFGAIGGISTRRATNEILTVLPYSNLAETERIMEDFIADFNR
                     QAVPSLEEGACKFALGGDVELTILAGAAEGQSRTDIDALLEQARSKQKEIGRLQCAPG
                     E"
BASE COUNT          865 a         1152 c         1031 g          952 t
ORIGIN      
        1 cgccgaaaaa cccgccgacc gcggatgcat tcttcaaaga cgtcttttcg gcgcccgact
       61 gaatccccgg gacggcggcg ggtacataag catccgcttc cgtataataa acaaaaggcc
      121 cggcatacag ttgaattcca taaggaaccg tcgcctgaag gccgatgccg aaattgacgt
      181 cccagagata ttttaccttt atatcagtca caaaaggaat tccgccgctg gttccgctaa
      241 cctcatcggt gaaattgctg aagttgcagc ttccctgcac aaaagcgccg acgccgaata
      301 cggaatgaac cggataatac gccttggcgc ccagcgtccc gaaaaatttc cagttttctt
      361 caaaagagtc ccgggaaacc gtggtcaggg ccgaagaaga gcgaaacgcg tccgatatct
      421 gcaaatcgga aatgccgatg cgcccgtaaa tctcccagcg gccggaggca ccgtacgccg
      481 cctgggaata gacctggtgc tggcgaatcc cataatccat gtcgcctttc aatgtatctt
      541 ccgcataccg gcagccgaca gcggtgttca cgccgccggt ttttttggac atcgtctgca
      601 gcggcccgaa cattccgccc gcaagcgcgg aagagcaaag gggaagaatc atcagggcta
      661 tcgtgaaaag aatgattttt ctcgtagggt ttctcctttt atgcgttctc agttggccgg
      721 cgcccgggtc gcctcacttt tccaattgcg gaaaaagagc gatcttgccc tgcgcgtctg
      781 gacttccttc atgcgatact gaatgtagcc gttgcgaatg gcaatgtaag ggtcgatggc
      841 cgcttcgatc accgcttcgt aatccccgag gctcaacgag gcgttgttga tcattttgaa
      901 gggtcttttg cccaggctgg tgtaccaggg ggtaaagtag gaaagcggat ccagaacata
      961 ctcgcccgcg atatccacgc tgtcccgggg gcttgacgga ccgagaaacg gccagacgat
     1021 gtaaaagccg tggcccacgc cgtacagacc cagcgtctgc ccgaaatcgg tgttctgttt
     1081 ttgcagttcg atgccccgat tggaggaggg atccagaagt cccccgatgc cccacaccgt
     1141 gttgatcgca aaacggcccg cttccgaacc ggcgccggtc aaatcggcct gcagcaggca
     1201 attgacgaag cggatgggaa acagcagatg gtaatagaaa tttttaacgc tgatccgcgc
     1261 cggttccgga accaccgctt tgtacccccg ggcaaccggt ttgagcagcc agaaatacag
     1321 ccggtcgttg aaccggtgca tggcgcggtt gaaaggttcg atcggatcgg cgatggtaac
     1381 gcgctcttcc gcaaaaggct cgtcgtcgta gtcctgattc tcatcgtctt tgtattcatc
     1441 ctgaaaagcg tcgtctgcac ggcccggcag cgcctcctcc ccgcggacag tcgcatccgc
     1501 cccggccgac ggagccttcg cgagggcgta atccatgctt ttcccattgc ccattaccca
     1561 tcgcccatcg cctactaccc cttgcccatc actcatcgcc ggcgcttccg cgcacgcgat
     1621 attcaggacc agacagcccg ggaagctccc ggccgcgaaa aaaaggactg tcaggagaaa
     1681 aacggacaga ttatgcttca tgatcctaat attaatgggt tttcaccttt ttgcgcaggg
     1741 tttccaggag ctgctccggc gtgccgccag ccagaatctc attgaactgg gttcggtagt
     1801 tctgcaccag gctcacattt tccacgacga catcgtaaac tttccacttg ccgtttttta
     1861 aaatcatccg gtagtaaatg ggaacttcct tggaagaggt gatcaccctg gactgaatct
     1921 ccgccttgtc gtccgcgatc atcgattcct tgtaaaaaac aattttctcg ttggaatatt
     1981 cgagaattct gtccaggtag gtcttttcca gcacctgttc aaaaagatca acaaactccc
     2041 tgcgctgctc cggggtgaac ttgttccagt tgcgggtcag tgtgcgccgg gaaaattcca
     2101 tctcgtcaaa catgtcttta tagatgacgc ggagtttttc cgtcttgagg gatttggccg
     2161 ccgggccttt catttttgga tcgcgcagaa cgtccaggac gcggttgacc gtgctttcca
     2221 cggcgctcat cggcgctccg gcatacgccg gaacggacat cagcagcatc atgaccagca
     2281 tgcaccccgc cagccgtttt ttcaggattc tgttttccat ctatttcttt tccttttcgt
     2341 tgttttgatt tttcccctgg tcctttttta tgtcgccaaa ggcgtatttg ctgatcagcg
     2401 attcaatatc caaagcggcc tgcgtgtccg tgatgacgcc gttgggctca agcaggtccc
     2461 ctcctccgcc cggatcgatg ctcagatact tgtcgccgat gaggccctcc gttttaatgg
     2521 aagcaatggc gtcatcataa atcttgatgt ccttccgaat ccggatttcc acgacggccc
     2581 tgaggtcttc ctggtccatc gaaatgcgct ccacgcgccc gacttcaatg cccaggatgt
     2641 tgaccggatt gcccgccttc agaccggtga cggttgtgaa tttggcgatg agcggatatg
     2701 tatctccgct gaaccagttc agtttcccga gtttaacggt catgtatccc acgcagatca
     2761 gtccgcaaac aacaaagata ccgaccatgg tttccatctt gtactttttc atcggttcat
     2821 tcccccgggg cgcattgcaa tcgcccgatt tctttttgtt tcgatctcgc ctgctcaagc
     2881 agggcgtcga tatcggtcct ggattggcct tcggccgcgc cggccaggat cgtcagctcc
     2941 acgtcgcccc ccagcgcaaa tttgcaggcc ccctcttcca gggagggaac ggcctgccgg
     3001 ttaaaatccg cgatgaaatc ttccatgatt ctttccgttt ccgcaaggtt ggaataggga
     3061 agcacggtca aaatctcgtt ggtggcgcgc cttgtggaaa tccctccgat ggcgccgaaa
     3121 tgccggtcga taaacagccc catattccgc agaatctcct gcgccgcgtc atggccgatc
     3181 tgtgaaacga tcgcgtcgag ctcccggagg ctgaaaatca ccaggcagaa ggtttctccg
     3241 cccttgctcc ttttgagctg cagatggttc agcagctcaa actggcgctt ggagtaaaga
     3301 ccggtcaatt ccttctgcag tccttccaga gagttgatga cttcgtccat gaacgggtgg
     3361 ttgaaatttt ccagctcctc cggcgtgccc tgaaaaacaa tccggtgatc atacagcgcc
     3421 agaatgcggt tggaaataaa atacacgtcc ggaatttcat ggctgaccag aaccgcggtg
     3481 aaattcagtt ttctctggtt ctgggcgatc atgctcaaaa tggcgttttt ccggacaggg
     3541 tcctggccgg tagtcggctc atcgaacaaa acgatccccg gatccgtaat caaagcgcgc
     3601 gccagcgcgg cccttttctg catgcctccg gaaagttccg acggatattt ggaagccgct
     3661 tccgtgagct ccgtctgttt catgcgggtc agcaccttgc gatggatctc cgtttttttc
     3721 aagttggtcg tctctatgag cggcagcgcg atattctcat aaaccgtcat ggaatcaaac
     3781 agggcgttgc cctgaaacat atagctgatc cggccgaagg atcccgcaag atccgaccgg
     3841 ctcatttcag caaccggttt tccccgaaac agaatgacgc catcatccgg ctggagcaat
     3901 ccgatgatat gtttgagcag cacgcttttg ccggagccgg aaaggccgat aatcgtcgtg
     3961 acctcccctt cgtagatctg gagattgacg cggtccagga
//
LOCUS       DAOPAF010000099         3974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_18524_length_3974_cov_46.195968, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000099 DAOPAF010000000
VERSION     DAOPAF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18524_length_3974_cov_46.195968"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PLQ77_07645"
     CDS             complement(<1..2002)
                     /locus_tag="PLQ77_07645"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPL10588.1"
                     /translation="MNQKMKTILQITPLKITLFVILIALALFLLDFKFLRLVELKTLD
                     LRIASRGDLKPGGETVIAVIDEKSLSELGRWPWPRTTIAQLVRKLKSEGAKAVGFDIV
                     FSEPDINSNLKTIDALSAEMKKRGVSQSGVTDLLRRKRAAADTDAILASAMRETGNVT
                     LGYFFHFARKGSDKELAHVTAQRIAQNARRIENSRYPMVNSTSGKPSDVFLPHAFAPE
                     ANIPALSVAGRNSGYFNALPDSDGSNRWSPLVIAFQNNYYSSLAVSLVQSYLDFPNLS
                     LNLEPFGAKSVVIDDIVVPTDESGQLLINYMGPPQTFPHYSISDILAGRLPKDTFRGK
                     IVLVGATAVGIYDLRVTPFSATFPGVEIHANVIDNILHRNFLIHSSVTRFIDVCSIVL
                     FGLILGLLIPRLRPITGMIAAFLMIAAFALINFFIFFSFNTWLNLVYPLITMATIYLG
                     ITIYHYFKEEREKKKIRGAFQYYLTSSVINEMLKDPDKLKLGGDKKDLTVLFSDIRGF
                     TTISEKMTPEELVALLNEYLTTMTNQVFHYDGLLDKYMGDAIMAVFGAPLDQPDHALR
                     ACRTSLAMMKELHKLQEKWKAEGRPAFDIGVGLNSGDMVVGNMGSEMRFDYTVMGDMV
                     NLGSRLEGINKEYGTNIIISEFTYDKVKDAMCCRELDGVRVKG"
     gene            2242..2538
                     /locus_tag="PLQ77_07650"
     CDS             2242..2538
                     /locus_tag="PLQ77_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10589.1"
                     /translation="MSKVKPGDVLNCEVCGLSVIVDEECGCAFADLICCEELMVNKGP
                     RPPKKAAAAPKKKTAAKQAKPAAKKPAAPKPAAKKPAVKKAAPKPAAKKKPAKK"
     gene            complement(2568..>3974)
                     /locus_tag="PLQ77_07655"
     CDS             complement(2568..>3974)
                     /locus_tag="PLQ77_07655"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPL10590.1"
                     /translation="AESFASIGAPVFLADVKGDLSGIAAAGVASEKLMKRLEGIGIKE
                     FHPRANTAVLWDVFGEKGHPVRATVSDMGPMLLARLLNLNDTQSGVLTLVFKIADDNG
                     LLLLDLKDLRAMIQFVGDNAKQFTTEYGNISAASIGAIQRGILTLDEQGGDKFFGEPM
                     LNIADLMQTDTEGRGVVNVLAADKLYNSPKLYSTFLLWMLAELFEQLPEVGDVEKPKL
                     VFFFDEAHLLFNDAPAALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPESVLGQLGNRV
                     QHALRAFTPRDQKAVKTAADTMRPNPAFNTADVITELGVGEALVSFLDEKGRPAPVER
                     SFVIAPGSRLGVLTDAERQAAVQRSTVYGFYEKTLDRESAYEKLRTSVAEAKTPAQPQ
                     AQTGKTEAGQEEPQAKGGGILGGAVGDLLFGRSGPRGGKTDGLVQASAKSLARTIGST
                     VGRQIVRGILGSLLGGKK"
BASE COUNT          853 a         1169 c         1156 g          796 t
ORIGIN      
        1 tgcctttgac gcggacgccg tcgagttcgc ggcagcacat ggcgtctttc actttgtcat
       61 aggtgaactc gctgatgatg atattggtgc cgtattcctt gttgatccct tccagccggg
      121 agcccaggtt gaccatgtcc cccatgaccg tgtagtcgaa gcgcatctcc gatcccatgt
      181 tgcccacgac catgtcgccg gaattcagac cgacgccgat gtcaaacgcg gggcgccctt
      241 ccgccttcca cttttcctga agcttgtgca gctccttcat catggccagg gaggtccggc
      301 aggcccgcag ggcgtggtcc ggctgatcca gcggcgcgcc gaagaccgcc atgatggcgt
      361 cgcccatgta tttgtccaaa agcccgtcgt aatggaacac ctggttcgtc atcgtcgtca
      421 ggtattcatt gagcagggcg accagctcct ccggcgtcat tttttcggaa atggtggtga
      481 agccccgaat gtccgagaac aggacggtca ggtctttctt gtcgccgccc agcttgagct
      541 tgtccgggtc cttcagcatt tcgttgatga ccgaagaggt cagatagtac tggaacgcgc
      601 cccggatttt tttcttttcc cgctcttcct tgaagtagtg gtaaatcgtg atgcccagat
      661 aaatcgtcgc catcgtgatc agcggataga caaggttcag ccaggtattg aagctgaaga
      721 aaatgaagaa attgatcagg gcaaaggcgg cgatcatcag gaatgcggcg atcattccgg
      781 tgatcgggcg caggcgggga atcaaaagcc cgagaatcag cccgaaaaga acaatcgagc
      841 agacatcgat gaaccgggtc acggaggagt ggatcaggaa attccggtgc agaatgttgt
      901 cgatgacgtt ggcgtgaatt tcaacgccgg gaaacgtggc ggagaaaggc gtcacgcgca
      961 gatcgtaaat gccgacggcc gtcgcgccga ccagcacgat cttgccgcgg aaagtgtcct
     1021 tcggaagccg tccggccaga atgtcggaga tggagtaatg gggaaacgtc tgtggcggcc
     1081 ccatgtaatt gatcagcagc tgccccgatt catccgtcgg aaccacgata tcgtcgatga
     1141 ccacgctttt ggcgccgaag ggctcgaggt tcagcgacag attgggaaaa tcaagatagg
     1201 actgaaccag ggaaaccgcc agggaagaat agtaattgtt ctgaaaagcg atcaccagcg
     1261 gcgaccagcg gttcgacccg tcgctgtccg gaagggcgtt gaaatagccg ctgttgcgtc
     1321 ccgcgacgga cagggcggga atattggctt ccggcgcgaa ggcgtgcggc agaaaaacgt
     1381 cgctgggttt gcccgacgtg gaattgacca tcggatagcg ggagttttct atgcgccggg
     1441 cgttttgtgc gatgcgctgc gccgtgacgt gcgcaagctc cttgtcggac cccttgcgcg
     1501 caaaatgaaa gaaatacccc agcgtgacat tgccggtttc tctcatggcg gaagccagaa
     1561 tcgcatccgt gtccgcggcg gctctttttc tgcgcagcag atcagtgacg ccggattggg
     1621 aaacgcctcg ttttttcatt tcggcggaaa gcgcgtcgat ggttttcaga ttggagttga
     1681 tgtccggttc ggaaaatacg atatcaaagc cgaccgcctt ggcgccttcg cttttcagct
     1741 tccgcaccag ttgcgcgatc gttgtccggg gccagggcca ccggcccagt tcggaaaggc
     1801 ttttttcatc gatgacggca atgacggttt caccgccggg cttcagatcg ccgcgcgacg
     1861 cgatgcgcaa atccagcgtt ttgagctcca ccaggcgcag aaatttaaag tccagcaaaa
     1921 aaagcgccag cgcgatcaaa atgacaaaaa gcgtgatctt cagcggcgta atctgcaaaa
     1981 tggtcttcat tttctgattc atgccgtccc ctcccccggg cgaaaatcat accagacgga
     2041 tgcgtaaagt caagctgtcc tttgtttcag ggcgctttgc ctttttcccg tttggcggcc
     2101 cgcagacgcc gggcgcgccc cccaaaaagc ggccccgcga attcaacgct tgaaagaaaa
     2161 acggttttgc actacaaaga acggcatcgg atacgtcagg atcggaagga agaactcaca
     2221 aacaacgagg aggaatttta aatgtcaaag gtaaagcccg gtgatgtttt gaattgcgaa
     2281 gtatgcggac tctcggttat tgtggatgag gaatgcgggt gcgcttttgc cgacctgatc
     2341 tgctgtgaag agctcatggt caataaaggc ccccggccgc cgaaaaaagc ggccgccgcg
     2401 ccaaagaaaa aaacggcggc gaagcaagcg aagcctgcgg ccaaaaaacc ggcggccccc
     2461 aaaccggccg ccaagaaacc cgcggtgaaa aaggccgccc ccaaaccggc agccaaaaag
     2521 aaaccggcaa aaaaataatc gggccggccc gccttggccg ggcgcgtcta tttttttccg
     2581 cccagcagag accccaggat cccgcggaca atctgccgcc cgacggttga cccgatcgtt
     2641 cgggcaaggc tcttggccga cgcctgaacc aggccgtccg tttttccgcc gcgcggcccg
     2701 gagcgtccga acagcaaatc ccccacggcg ccgcccagga tgccgccgcc cttggcctga
     2761 ggctcctctt ggccggcttc ggttttgccg gtttgagcct gcggctgcgc cggagtcttc
     2821 gcttccgcga cggaggtccg cagcttttcg taggcggatt cgcggtccag cgttttttca
     2881 tagaaaccgt aaaccgttga ccgttgaacg gccgcctgcc gctcggcatc ggtgagcacg
     2941 ccgagccggg agccgggcgc gatcacaaaa gacctctcca ccggcgccgg acggcctttt
     3001 tcgtccagaa atgaaaccag cgcctctccg acgcccagct ccgtaatcac atccgccgtg
     3061 ttaaaggcgg gattgggccg catggtgtcc gccgccgttt tcaccgcctt ctgatcgcgc
     3121 ggcgtgaagg cgcgcaaggc gtgctggacg cggttcccca actgccccag gacgctttcc
     3181 ggaatatcga gaggattctg cgtcacgaaa tacactccca cccccttgga gcggatcagc
     3241 cgcaccacct gctcgatctt ttccagaaga gcggccggcg cgtcgttgaa cagcaggtgc
     3301 gcctcgtcaa aaaagaacac cagtttgggc ttttcaacat cccccacttc cggcaactgt
     3361 tcaaagagtt cggccagcat ccacaaaaga aacgtggaat acagcttggg gctgttgtag
     3421 agcttgtcgg ccgccagcac gttgaccacg ccccgcccct cagtgtccgt ctgcatcagg
     3481 tcggcaatgt tcagcatcgg ttctccgaag aatttgtcgc cgccctgctc gtccagcgtc
     3541 agaatccccc gctgaatcgc tccgatggaa gccgcggaaa tgttgccgta ttccgtggtg
     3601 aactgtttgg cgttgtcgcc cacgaactga atcatggccc gcagatcctt cagatccagc
     3661 agcagcaggc cgttgtcgtc ggcaatcttg aaaaccagcg tcagcacgcc cgactgggta
     3721 tcgttgagat tgagcagcct cgccagcagc atcgggccca tgtcggagac ggtggcgcgc
     3781 accgggtgcc ccttttcccc gaacacgtcc cataacacgg ccgtgttggc gcgcgggtgg
     3841 aattccttga tgccgatccc ttccaggcgc ttcatcaatt tctcggaagc gacgcccgca
     3901 gcagcgatgc cggagaggtc gcccttgacg tcggccagaa aaaccggcgc tccgatggac
     3961 gcgaaggatt cggc
//
LOCUS       DAOPAF010000100         3709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_20132_length_3709_cov_74.171045, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000100 DAOPAF010000000
VERSION     DAOPAF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20132_length_3709_cov_74.171045"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..>3709
                     /locus_tag="PLQ77_07660"
     CDS             229..>3709
                     /locus_tag="PLQ77_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPL10591.1"
                     /translation="MYSLGINIGSSNVKAALLEGRSVVWSAVEPHEGNFPDTLKKMLS
                     GRDWPAGIPTLATGTEGRRLLNIPGVIEPLCIEAALQERGGSVDALVSLGGEDLVVYT
                     IDSNNKIITSFSGNKCASGTGEFFRQQLARMNMTLGDIRRIPDNCRVLKLSSRCSVFM
                     KSDCTHRLNKGEATTGDIALSLSDVMAVKVVDFLKRARIEKGKVLLVGGVTQNPYILR
                     FIRERMPEIEFIVPEQAPWFEAYGAALMAASAGSPLPAPEKLFRQSSVQFKRFKNLKS
                     AEGRVTYLPSKKGKVVAGREYILGVDGGSTTTKASLIDFETSEVTASFYGRTHGDPVR
                     ALKHCLREIQKQIREEIGDARIRITLASTTGSSREILGVFLETPAVYNEIIAHAVGTS
                     FYRDRIDTIFEIGGQDAKYVSLKNKVPIDYAMNEACSAGTGSFLEESAQGDLNIPEAA
                     MIGDIALAADAPLKFGEHCSAFINSDIRNAIQQGASREDITAGIVTSIVSNYLNRVVG
                     NRTIGRNIVLQGGVAKNKAVPLAFAMLLDKNILVPPDPELMGCFGVGLLARQKFEEGI
                     LTKQAYDIDAILATDIVYEKEFPCKACDNFCPIRVLLVNGHRYMFGGRCNKYANLRKK
                     KAFDEASVADYIEKRNDILFRECAPDPGALARKKDVIVGIPRCFSIFTLWPFYSWFFH
                     TLGVETLLSEEIPQEGTARVESSYCFPAEIAHGAVQDIFNRKVDYIFLPHFRDMDSYE
                     KAAPANFCPITQSLPYYIQKAFPEIPEEKYLTPVVSFMYGLEKAAEPFVAMAADLGFG
                     EGEAKNAFAVAHEKQMECFAKFAELGRQALEEARRAKRPVIAVLGRPYNSFTTDANMG
                     IPRKYTSRGYSVIPFDILPFADQVIYPNMYWYYGQQDMKAGTLLKNEDNIYITYISNF
                     SCAPDSFMLHYLKWIMGVKPFLILELDSHTADAGVDTRIEAFLDIIEGYRSKIDDIRE
                     ERYDNGLRFINRPGEEIHILDSATGEKIPIKGNPRVKLLLSNMGRLSAELIGATIRGL
                     GINARTMPVPDIYTLQMARNHASGKECLPSHLVLGSALKYLSSDQYRKDEIYILFVPT
                     TTGPCRTGQYFVFYENLFRDLRLENVVVFTLEADNSYTELGPEFTKYAWWAAVIGDYM
                     KDVETSLRTCAADP"
BASE COUNT          821 a         1135 c         1020 g          733 t
ORIGIN      
        1 gcgatggagg aaggacactg atccttgcgg gtggattatc ccccccttct acaatcacaa
       61 aggaacaaaa gcagctggcg gcggacgcaa ccagctgctt ttttttgccg gacggcaaat
      121 ttaatgcgtt gatattcgga ggcatttaag aaacattcct ctctgcactt gacgatgccg
      181 cgcaatcgta ctattgccca tgcgaaggat tcttttactg gagtgattat gtacagcctg
      241 ggaatcaaca tcggctcctc gaacgtcaag gccgctctgc tggaaggccg cagcgtcgtg
      301 tggagcgccg tggagccgca cgaaggcaac tttcccgata cgctcaaaaa gatgctttcc
      361 ggccgcgact ggcctgcggg cattccgacg ctggccaccg gcaccgaagg acgcaggctt
      421 ttgaacattc ccggcgtcat cgaaccgctc tgcatcgagg cggcccttca ggagcggggg
      481 ggttccgtcg acgcgctcgt ctccctgggc ggcgaagacc tggtggtcta tacgattgat
      541 tcgaacaata aaatcatcac cagtttttcc ggcaacaaat gcgcgtcggg caccggcgaa
      601 ttcttcaggc agcagctggc ccgaatgaac atgacgctcg gcgacattcg ccgcattccg
      661 gataactgcc gcgtcctgaa gctctcgtcg cgctgttcgg ttttcatgaa aagcgactgc
      721 acgcaccgcc tgaacaaggg cgaagccacg acgggcgata ttgccctttc cctgtccgac
      781 gtcatggccg tcaaggtcgt cgatttcctc aagcgggcgc ggattgaaaa aggaaaagtt
      841 ctgctggtcg gcggcgtcac gcaaaacccg tacattctgc gcttcattcg ggagcgcatg
      901 cccgagatcg aatttatcgt ccccgaacag gcgccctggt tcgaggctta cggcgcggcc
      961 ctgatggccg cttccgcagg cagcccgctt ccggccccgg agaagctgtt cagacaaagc
     1021 tctgttcagt tcaaacggtt caaaaacctg aaaagcgctg agggcagagt cacctacctg
     1081 ccgtcgaaga aaggaaaagt cgtcgccgga cgagaatata tccttggcgt ggacgggggt
     1141 tccaccacga cgaaagccag cctcatcgac tttgaaacca gtgaagtcac cgcgtccttc
     1201 tacggccgga cgcacggcga tccggtgcgc gccctcaaac actgccttcg ggagattcag
     1261 aaacagattc gggaggagat cggagatgct cggatccgca ttacgctcgc gtccacgacc
     1321 ggatcctcgc gcgaaattct cggcgttttt ctggaaacgc ccgccgtgta caacgaaatc
     1381 atcgcccatg ccgtcggcac ttccttctac cgcgaccgca tcgacaccat ctttgaaatc
     1441 ggcggccagg acgccaagta tgtgtcgctg aaaaacaagg tgccgatcga ctacgcgatg
     1501 aacgaagcct gctccgcggg caccgggtcc tttctggaag aatcggcgca gggcgatttg
     1561 aacattcccg aagcggccat gatcggcgac atcgcgctgg cggccgacgc gccgttgaaa
     1621 ttcggcgagc actgctccgc gttcatcaat tccgacatcc gcaacgcaat tcagcagggc
     1681 gcttcccgag aggacatcac ggcgggcatt gtcacctcca tcgtatccaa ctacctcaac
     1741 cgggtggtgg gcaaccggac gatcggccgg aacatcgttc tgcagggagg cgtcgccaaa
     1801 aacaaggcag tgccgctggc tttcgcgatg cttctcgaca agaatattct ggtgccgccc
     1861 gaccccgaac tgatggggtg cttcggcgtc ggcctgctgg cccggcagaa atttgaagaa
     1921 gggattctga ccaagcaagc ctatgacatc gacgcgattc tggccacgga catcgtctat
     1981 gaaaaggaat ttccgtgcaa ggcctgcgac aatttctgcc cgatccgcgt gctcctggtc
     2041 aacggccacc gctacatgtt cggcggacgc tgcaacaaat acgcgaacct tcggaagaaa
     2101 aaagccttcg acgaagcctc cgtagcggat tacatcgaaa aacgaaacga cattctcttt
     2161 cgggaatgcg cccctgatcc gggcgcgctg gcccgcaaaa aagacgtgat cgtcggcatt
     2221 ccccgctgct tttccatttt cacgctctgg ccgttttatt cctggttttt ccacacgctg
     2281 ggcgtcgaga cccttctgtc cgaggaaatc ccgcaggagg gaacggcccg cgtggaaagc
     2341 agctactgtt tcccggcgga aatcgcgcat ggcgccgtcc aggacatctt caaccggaag
     2401 gtggactata tctttctgcc gcatttccgg gatatggaca gctacgagaa ggcggcgccc
     2461 gccaatttct gccccatcac gcagagcctg ccctattaca tccaaaaggc ttttccggag
     2521 attccggaag aaaaatacct gacgccggtc gtcagcttca tgtacgggtt ggaaaaagcc
     2581 gcagaaccgt ttgtcgccat ggcggcggat ctggggttcg gcgaaggcga agcgaaaaac
     2641 gcctttgccg tcgcccatga aaagcaaatg gaatgctttg ccaaattcgc cgaactgggg
     2701 cgtcaggcgc tcgaagaggc gcgccgggcg aaaagacccg tcatcgcggt tctgggccgc
     2761 ccctacaact cctttaccac cgacgccaac atgggcatcc cccggaaata cacttcccgc
     2821 ggctactcgg tcatcccctt tgacattctg ccgtttgccg accaggtcat ctaccccaac
     2881 atgtactggt actacggcca gcaggacatg aaggccggca cgctcctgaa aaacgaagac
     2941 aacatttaca ttacctacat ttccaatttc tcctgcgcgc ccgactcctt catgctgcat
     3001 tatctgaagt ggattatggg cgtaaagccg tttctgattc tggagctgga ttcccacacg
     3061 gccgacgccg gcgtggacac gcgcatcgag gcgtttctgg acatcatcga aggctaccgc
     3121 tccaaaatcg acgacatccg cgaggagcga tacgacaacg gcctgcgctt catcaaccgt
     3181 cccggcgagg aaatccatat tctcgacagc gccaccggcg aaaaaatccc gatcaaggga
     3241 aatccgcggg tcaaacttct tttgtccaac atgggccggc tctccgcgga gctcatcggc
     3301 gcgaccatcc gcggcctggg catcaacgcc cgaaccatgc cggtgccgga catttacacg
     3361 ctgcagatgg cgcgcaatca cgcctccggc aaggaatgcc tgccctcgca tctggtgttg
     3421 ggcagcgcgc tgaaatatct gtcctccgac cagtaccgca aggatgaaat ttacatcctc
     3481 ttcgtgccga cgaccactgg cccctgccgg accggccagt acttcgtgtt ctatgaaaac
     3541 ctcttccggg acctgcgcct ggaaaacgtc gtggtcttca cgctggaagc ggacaattcc
     3601 tacacggagc tggggccgga attcaccaag tacgcctggt gggcggccgt catcggcgac
     3661 tatatgaagg atgtggaaac ctcgcttcga acgtgcgccg ccgatcccg
//
LOCUS       DAOPAF010000101         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_20396_length_3673_cov_40.345771, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000101 DAOPAF010000000
VERSION     DAOPAF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20396_length_3673_cov_40.345771"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ77_07665"
     CDS             <1..251
                     /locus_tag="PLQ77_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10592.1"
                     /translation="ITMARWKYKTSIIDLDRLIPPEAISCNDTGTCVIDGIPGSQDHF
                     KGILDKEGESEWELIQCQPHGQKLLCIWKKKVAEAFAS"
     gene            428..1309
                     /locus_tag="PLQ77_07670"
     CDS             428..1309
                     /locus_tag="PLQ77_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPL10593.1"
                     /translation="MAFNPPAKMVALVGNAGVGKAKLSIFNLLLRGFMAGAYIAIGAA
                     LCTVCSTGAAPILGAGMAKLISGAVFPVGLIAIVLTGMELFTGDCMIVPVAAMMKKVT
                     WGAVMRNWVWVYIGNFVGSLAYAYVMVIGPFVTGQPDGTMAVNAFGDNAVGIAVAKIL
                     EYKAVGGAGLWSCFIKAIGCNFLVNIAILLAITADDIIGKFFGIWFPIMAFVATGFEH
                     CVANMYFLPAGKFLTDWYPAVIAKKGGLLLANGGLSWGDIWIWNLIPATIGNIFGGFL
                     FIGVTYFLMFRNDLPKD"
     gene            1319..1789
                     /locus_tag="PLQ77_07675"
     CDS             1319..1789
                     /locus_tag="PLQ77_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPL10594.1"
                     /translation="MSVFAWIVFFICAGAVALNYAATGNASFLWYAIPGLILLLVIPM
                     TLAWMSRRSFTQARQQSEHQARSCKIGKIVPAMIGNVVRISGEVRKISFKWLNRPHFH
                     IQDATGLIRVIMFTAPANKVAAGDKVEAIGIVMKYPLSKERIAISAVSVKRIEN"
     gene            1800..1940
                     /locus_tag="PLQ77_07680"
     CDS             1800..1940
                     /locus_tag="PLQ77_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10595.1"
                     /translation="MDSREGRCIFGFLLLGLAPVLATAGGAGAGMILFAAGLLVAITA
                     VG"
     gene            2022..2585
                     /locus_tag="PLQ77_07685"
     CDS             2022..2585
                     /locus_tag="PLQ77_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HPL10596.1"
                     /translation="MSDNLANPAPLGLMGFGMTTVLLNIHNAGLFEMSAVILAMGIFY
                     GGIAQIIAGCMEFKKGNTFGTTAFTSYGLFWLTLVFIIYFNGKFGIPANTMGFLACYL
                     FMWGLFTFFMWIGTWKANRGLQVVFLSLTILFWMLAARDAFGWTGTWAVLTGYEGIFC
                     GFSAIYCAMAQVINENFKRVVLPLGQK"
     gene            complement(2693..3490)
                     /locus_tag="PLQ77_07690"
     CDS             complement(2693..3490)
                     /locus_tag="PLQ77_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPL10597.1"
                     /translation="MDVLQAIILGLVQGLGEFLPISSSAHLVLVPWLMNWPYSGLTFD
                     IALHVGTLIAVVIYFWKDWLRLLHKGFTKPKEREGKLFWYLVLATIPGALIGFLLEDV
                     AETVFRHPVLIACMLILLGVILYVVDRRGKKQIAVDHISLKTSLLIGLSQALAIIPGV
                     SRSGVTMTAALALGMTREGAARFSFLLSAPIILGAALVKAPELAANPSMVDAAFLTGM
                     AVACLSGLAAIGFLLRYVQTRTFLPFVAYRFIAGALVIGVAWFRCAS"
BASE COUNT          855 a          997 c         1008 g          813 t
ORIGIN      
        1 gtatcaccat ggcaagatgg aagtacaaaa caagcatcat cgatctggac cgcctcatcc
       61 cgcccgaggc catttcctgc aacgatacgg gaacctgcgt cattgacggc atcccgggca
      121 gccaggacca cttcaaggga attctcgaca aggaaggcga gagcgagtgg gagctgattc
      181 agtgccagcc ccacggacaa aagcttctct gcatctggaa gaaaaaagtc gcggaagcct
      241 ttgccagtta aagtgcagct ctccgcatct ttttcgcatc cacgccgact tcagattcaa
      301 accggccttt caggcacggt ccccgcgcgt tggtgcgttt atttcagcga aacatcatta
      361 tgcgaagcgg ccgtgcaggg gacggtcctt ttgcatgaaa aatcaaaaat acaggaggta
      421 atatttcatg gctttcaatc cccccgcaaa aatggtcgct ctcgtcggta acgcgggagt
      481 cggcaaggca aaactttcaa tctttaatct gctgcttcga ggattcatgg cgggcgccta
      541 catcgccatc ggagcggcgc tgtgtaccgt ctgctcaacc ggagcggcgc cgattctcgg
      601 agcgggcatg gccaagttaa tcagcggcgc ggtcttccct gtcggcctga ttgccatcgt
      661 cctcaccggc atggagctct tcaccggcga ctgcatgatc gtccccgtgg ccgccatgat
      721 gaagaaggtc acctggggcg ccgtcatgag aaactgggta tgggtttaca tcggaaactt
      781 tgtcggctcg ctggcctacg cgtacgtcat ggtgatcgga ccctttgtca cgggacagcc
      841 ggacggcacc atggccgtga acgcattcgg ggacaacgcg gtcggaatcg ccgtggcgaa
      901 aatccttgaa tacaaggccg tcggcggcgc gggcctatgg tcctgcttca tcaaggccat
      961 cggctgtaac ttcctggtca acatcgccat tctgctggct attacggcgg acgacatcat
     1021 cggaaaattt ttcggcatct ggttcccgat catggccttc gtcgcaaccg gcttcgagca
     1081 ctgcgtggcc aacatgtatt ttcttccggc gggaaaattc ctaaccgatt ggtacccggc
     1141 cgtcatcgcg aagaaaggcg gactgcttct ggccaacgga gggctttcgt ggggcgatat
     1201 ctggatctgg aacctgatcc ccgcaaccat cggaaacatc ttcggcggat tccttttcat
     1261 cggggttacc tacttcctca tgttcaggaa tgatctgccc aaggactaga gggaacccat
     1321 gtccgtattt gcctggatcg tcttttttat ttgcgcggga gcggtggcgc tgaattacgc
     1381 cgcgacggga aacgcgagct ttttgtggta tgccatcccc ggcctgattc tgcttttggt
     1441 catcccgatg accctggcct ggatgagccg cagaagtttc acgcaggccc gccaacagtc
     1501 tgaacatcag gcgcgctcct gcaagattgg aaaaattgtc ccggcaatga tcggcaatgt
     1561 agtgcgaata tcgggtgagg ttcggaaaat cagcttcaaa tggctcaacc ggcctcactt
     1621 tcacattcaa gacgcgacgg gattgattcg ggtcatcatg ttcaccgcgc ccgccaacaa
     1681 agtggcggcg ggcgacaagg tggaggccat cggcattgtg atgaaatatc cgctgtcgaa
     1741 agaacggatc gccatatcag ccgtcagcgt caaacgtatc gaaaattaag gagacgatta
     1801 tggactcaag agaaggaaga tgcatcttcg ggtttctttt gcttggattg gcccctgttc
     1861 tggccacggc aggcggagcg ggcgcgggaa tgattctgtt tgccgcgggt ttgctggtcg
     1921 cgatcacggc cgtcggttag aaaatcattt gcggaagaaa gtaacggcgc aatccgccgt
     1981 tactttcccc tgacgcattt taaacattca aggagtattt tatgtctgac aatcttgcta
     2041 atccggcccc gcttggtttg atggggtttg gaatgaccac ggttcttcta aacatccata
     2101 acgcgggcct gtttgagatg agcgcagtga ttctggccat gggcattttc tatggaggaa
     2161 tcgcgcagat tatcgcgggc tgcatggaat tcaaaaaagg caatacgttc ggcacgaccg
     2221 cttttacgtc ctacgggctc ttctggctga cccttgtgtt catcatttac ttcaacggaa
     2281 aattcggcat acccgccaat acgatgggat ttctggcgtg ttatctcttt atgtggggcc
     2341 tgttcacatt tttcatgtgg atcggcacat ggaaggccaa tcggggcctg caggttgtct
     2401 ttctgtcact gacgattctg ttttggatgc tcgcggcgcg cgacgcgttc ggctggaccg
     2461 gaacatgggc ggttttaacc ggctacgaag gcatcttctg cggattttcg gccatttact
     2521 gcgcgatggc gcaggtaatc aacgaaaatt tcaaaagagt ggtactgccg ctggggcaga
     2581 aatagattga attccattgc ggtcggaata aaattctgat ccatcgatcc tgaaggaaaa
     2641 aagaggctct cccgtgatca taacggggga gcctttttca ctggaccggg atttatgaag
     2701 cgcacctgaa ccaggcgacg ccaatcacca gagcccccgc aataaaccga tacgccacaa
     2761 acggcagaaa cgtccgggtc tgaacgtagc gaagcagaaa gccgatggcg gcaaggccgg
     2821 agaggcacgc caccgccatg ccggtcagaa atgccgcatc caccatggaa ggattggcgg
     2881 caagctccgg agcttttacc agcgcggccc ccagaatgat cggcgccgag agcagaaagg
     2941 aaaagcgcgc cgcgccctcg cgggtcatgc cgagcgccag cgctgccgtc atcgtaacgc
     3001 cggagcggga aacgccggga ataatggcca gcgcctgtga caaaccaatc agcaaactgg
     3061 ttttgagcga aatatgatcc acggcaattt gttttttacc ccggcggtca accacataaa
     3121 gaataacgcc cagaaggatc agcatgcagg cgatcagaac ggggtggcgg aaaaccgttt
     3181 cggcaacatc ctcgagcaaa aagccgatca gcgcgccggg aatcgtcgcc aggaccagat
     3241 accagaagag ctttccttcc ctttcttttg gtttcgtaaa ccccttatgc agcagcctga
     3301 gccagtcttt ccagaaatag atcaccaccg caatgagggt gccgacatgc agggcgatat
     3361 caaacgtcag gccggagtac ggccagttca tgagccaggg aaccagcacc agatgcgcgg
     3421 agctcgagat gggaagaaat tcccccagcc cctgcaccaa accgagaatg atcgcctgca
     3481 aaacgtccat gaccgctcct ttagcctctt ttcgccgtgg ccgttgcctt acctgataaa
     3541 accggttttg tccacccctg ccggccagga aacagcggaa aaaaaatcct ttgcacctct
     3601 tctgatcatt tctttcaatc cgttgaacat ttcattgaaa gaaaaacgcc gaaacgattg
     3661 ttttacgccg ggt
//
LOCUS       DAOPAF010000102         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_20438_length_3668_cov_71.608912, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000102 DAOPAF010000000
VERSION     DAOPAF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20438_length_3668_cov_71.608912"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLQ77_07695"
     CDS             complement(<1..491)
                     /locus_tag="PLQ77_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPL10598.1"
                     /translation="MDLGEIFAYAFVQRAMLAGALIAAVSALLGLFLVLRRFSLIGDG
                     LAHTTFGSVAVVLLVGVSPFYVTLAALPLVMVSSLAIYKLTSSKKINADAAIGIVSSL
                     GIAAGITLSSLSGGFNVDLFSYLFGNILTVNRTELVLSMIVFAVAAAVVFYYYDDLFA
                     VTF"
     gene            complement(491..1228)
                     /locus_tag="PLQ77_07700"
     CDS             complement(491..1228)
                     /locus_tag="PLQ77_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPL10599.1"
                     /translation="MPVIRVEDLTFRYNGTEVINDIGFTVERGVYLAVVGPNGSGKST
                     LVKNILGILKPEKGRVELFGRRLEDFSSWGKIGYLPQRLSGLNTHFPGTVAEIVQMGL
                     PKKDPALLSDTLDMMAIGHLRRRLIGDLSYGEQQRVMLARALIRRPELLIFDEPTTAL
                     DPETRDVFYALTQKLNRQEGTTVALVTHDSGVVGQYAQHLLYLDKKVIFSGTFRDFCA
                     SADMTGFFGATSQHIICHQHDSTGSGR"
     gene            complement(1219..2187)
                     /locus_tag="PLQ77_07705"
     CDS             complement(1219..2187)
                     /locus_tag="PLQ77_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPL10600.1"
                     /translation="MIAKKMRLIFFIGLALIWSCAPAEEPAGKDGRLKVIATIFPLYD
                     FARNIGGDRISVTMLLPPGSDVHHYELKPNDIIRVSKADIFLFTSFDLEQWAYKIINA
                     AAEKTNMLAVETGQGAFLLPLPESPELDPDHSAAPQANDPDHASKYDPHIWLDAANAQ
                     KMIDNIAAAFIIRDPKNSEVYKENAGRYKRRLTELDEKYRVSLSACKTKTVLHAGHWA
                     FAYLANRYHLRYQSAYNSSADAEPSPQQILALMEQVRAQKLRYIYYEDLTAPKLALMM
                     ASETGVELLKLNNGHDVGKKDFQAGETFLSLMEKNLVNLKKGMQCR"
     gene            complement(2236..2889)
                     /locus_tag="PLQ77_07710"
     CDS             complement(2236..2889)
                     /locus_tag="PLQ77_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10601.1"
                     /translation="MTAATEQALNGLRDLSTLQWYVIPLLAIVFYVYTREMKEARRTG
                     DWNAVLAGLTIFGVDFFNETWNGWVLHFTGRSAFWTTPGETALRTMVGWNIEIMFMFL
                     LVGIIFYNTLSEGKKEKILGLPEKWFWAIGYSVFCVFVECLLNIGGHLVWEYPFWYLS
                     FQGVWLIFLIGYFHFFCFAILVISLKTMKAKLATLGIIYAVPVIMNVLAFGFWGWNY"
     gene            complement(2920..>3668)
                     /locus_tag="PLQ77_07715"
     CDS             complement(2920..>3668)
                     /locus_tag="PLQ77_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPL10602.1"
                     /translation="LLETAYLSNPQEEKLLKTYKFRRTIAQAVALSVDRYFAGSGAVA
                     QTPAEDEKGNRQITTSEKTAGAEGVAGAAGAAGAEDRMKTAAYTVRKGESLHAIALKH
                     KTNLATLLTLNKMKLNDPLYAGRKILVPAVETAIVEERIKTTPYTVRKGESLHAIAQR
                     HETDIATLLKLNNMKINDPLYAGRKILVPAAGEEAAGKRRLKKYTVQKGDTLFLLAKS
                     SSVTIEELRRINKMTETDVLRVGQRIHLPE"
BASE COUNT          846 a         1072 c          922 g          828 t
ORIGIN      
        1 tcaaaggtta ccgcgaacag atcatcataa taataaaaca ccaccgccgc cgccaccgca
       61 aagacgatca tggaaagaac cagttcggtg cggttcacgg tcagaatatt cccgaaaaga
      121 taactgaaca aatccacatt gaaaccgccg gaaagactgg acagagtaat gcccgccgca
      181 attcccaggg aggaaacgat gccgatggcg gcatcggcgt tgatcttttt cgatgacgtc
      241 agcttgtaaa tcgccaggga ggaaaccatc accagcggca gcgccgccag cgtgacataa
      301 aagggactga cgccgacgag cagtacgacg gctacgctgc cgaaggtggt atgcgcaagg
      361 ccgtctccga tcagagagaa acggcgcaaa acaagaaaca gccccagaag cgccgaaacc
      421 gcggcaatca gggcgcctgc aagcatcgcc cgctgaacaa atgcgtatgc aaaaatctcc
      481 cccaggtcca tcagcggccg ctccctgtgg aatcgtgttg atggcagatg atgtgctggc
      541 tggtcgcgcc gaaaaatccg gtcatgtccg cagaagcgca gaaatcacga aatgtcccgg
      601 aaaagattac tttcttgtcc agatacagca aatgctgcgc atattgaccg accacgccgc
      661 tgtcatgggt caccagcgcc accgtcgtgc cttcctgacg gttcaacttc tgtgtcagcg
      721 catagaagac gtccctggtt tcggggtcga gcgcggtggt cggttcgtcg aagatcagca
      781 attccggccg ccggatcagc gcccgcgcca gcatgacccg ctgctgttct ccgtaagaca
      841 gatcgccgat caaacgccgg cgcagatggc caatcgccat catgtccagc gtgtccgaca
      901 gcagggccgg gtcttttttc ggcaaaccca tctgcacgat ttccgccacc gtgccgggaa
      961 agtgcgtatt caaaccgctg agccgctgcg gcaaataacc gattttcccc cacgaactga
     1021 aatcttcgag ccgcctgccg aacaactcca cccttccctt ctccggtttc agaatcccca
     1081 gaatattttt gacgagcgta cttttccccg atccattggg acccacgacg gccagataga
     1141 cgcctctttc gacggtaaaa ccgatatcgt tgatgacctc cgttccattg taacgaaacg
     1201 tcaaatcctc tacgcgaatt accggcattg catccccttc ttcagattga ccagattttt
     1261 ttccatcagc gacaaaaacg tttcgcccgc ctgaaaatct tttttgccga catcatgccc
     1321 gttgttgagc ttgagcagct ccacgccggt ttcggaggcc atcatcaagg cgagcttcgg
     1381 ggccgtcaga tcttcgtaat aaatatagcg caacttttgc gccctgacct gctccatcag
     1441 cgccaggatc tgctgcggag aaggctccgc atccgccgag ctgttgtacg ccgattggta
     1501 gcgcagatga taccggttgg ccagatacgc gaaggcccag tgccccgcat gcaaaacggt
     1561 tttggttttg cacgcggaaa gcgaaacgcg gtatttttca tccagctccg tcagccggcg
     1621 tttatagcgt ccggcgtttt ctttatagac ttcgctgttt ttcggatccc ggatgatgaa
     1681 agccgccgcg atattgtcga tcattttctg cgcgttcgcg gcgtccagcc agatatgcgg
     1741 atcatatttg gacgcatggt ccggatcgtt tgcctgcggg gccgctgagt ggtcgggatc
     1801 aagttcggga gactccggca agggcagcag aaacgcgccc tgcccggttt cgaccgccag
     1861 catattcgtt ttctcggcgg ccgcattgat gatcttatag gcccactgtt ccagatcaaa
     1921 gctggtaaaa agaaaaatat ccgctttgct gacccggata atgtcgttgg gtttcaattc
     1981 gtaatggtga acgtccgacc ccggcggcag cagcatggtc accgaaattc tgtccccccc
     2041 gatattgcgg gcaaagtcgt acaggggaaa aatcgtggcg atcactttca gccggccgtc
     2101 cttcccggcc ggctcttccg ccggggcgca ggaccagatc agcgccagtc cgatgaaaaa
     2161 tatcaaccgc atcttttttg caatcaagcg aatccgcctc tctttgggcc tagcggctcc
     2221 aacggttcgt cgtcatcagt aattccatcc ccagaagccg aaggccagca cgttcatgat
     2281 cacgggtacg gcgtaaatga tgcccaacgt tgccagtttg gccttcatgg ttttgaggct
     2341 gatgaccaga atggcaaaac agaaaaaatg gaaataaccg atcaggaaaa tcagccagac
     2401 cccctgaaac gagagatacc agaagggata ctcccagacc aggtgtccgc cgatgttcaa
     2461 gagacattca acgaagacgc aaaaaacgct gtaaccgatc gcccagaacc atttttcagg
     2521 caatcccaga atcttttctt ttttgccctc cgacagggta ttgtagaaga tgatgcccac
     2581 cagcagaaac atgaacatga tttcaatgtt ccagccgacc atcgtgcgca gcgccgtctc
     2641 gccgggtgtg gtccagaaag cggagcggcc cgtaaaatgg agaacccagc cgttccaggt
     2701 ctcattgaag aaatcgacgc cgaagatggt caacccggcc agaacggcgt tccagtcgcc
     2761 tgttctcctg gcttccttca tttcacgggt gtacacataa aacacgatcg ccagaagcgg
     2821 aatcacatac cactgaagcg tggaaagatc gcgcagaccg tttaacgcct gttcggttgc
     2881 cgccgtcata aaagcctcct ttcaaacctt cgttttctct tactcgggca gatgaattct
     2941 ctgccccacc cgcagaacat ccgtttccgt cattttattg atgcgccgca attcctcaat
     3001 ggtgacggaa ctgctcttcg ccagcaaaaa caacgtgtcg cctttttgga ccgtgtattt
     3061 tttcaggcgc cttttaccgg ctgcttcttc cccggcggcg ggaacaagaa ttttccgccc
     3121 ggcatacaga ggatcgttga tcttcatgtt gttcagtttc aaaagtgtgg cgatgtccgt
     3181 ctcgtgcctt tgggcaatcg catgcagact ctcacccttc ctgaccgtgt aaggggtcgt
     3241 ttttatccgt tcttccacaa tcgccgtctc aacggcggga acaagaattt tccgtccggc
     3301 atacagagga tcgttgagct tcatcttatt cagagtcaaa agcgtggcaa ggttcgtctt
     3361 gtgctttagg gcaatcgcat gcagactctc acccttcctg accgtgtaag cggccgtttt
     3421 catccggtct tccgcgcccg ccgccccggc agcgcctgcg accccctccg caccggcagt
     3481 cttttccgat gttgttattt gccggtttcc tttttcgtct tccgcaggag tttgtgccac
     3541 agctcccgat ccggcgaagt accggtcaac ggataatgcc accgcctgcg caatcgttct
     3601 tcgaaacttg taagttttca gcagcttttc ttcctgcgga ttggaaaggt acgcggtttc
     3661 cagaagca
//
LOCUS       DAOPAF010000103         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_21059_length_3577_cov_47.332765, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000103 DAOPAF010000000
VERSION     DAOPAF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21059_length_3577_cov_47.332765"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1675
                     /locus_tag="PLQ77_07720"
     CDS             260..1675
                     /locus_tag="PLQ77_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL10603.1"
                     /translation="MDKRDFPSNVYDGMALIADPIHSYAFFTAPRESDPAEKTEKDII
                     DSPWMQRLRRIYQLQSARWVYPAAEHTRFQHSLGTMHVAGEFARHLYPSLRQVCRDAP
                     SMHYVEELLRLAGLLHDVGHGPFGHFFDEHYLSGWALTHEDMGRLIIVKKLGPLLAKI
                     SRSPSGPFARGEKLDPRQVAFLIKMPEEEEPRQPRWLVWLRQLFSGIYTVDNLDYVQR
                     DAYMTGFSLDMVDVPRLRYYTFFTKDGLALHQSGISALRRFLNARLNLYSNVYFHRTT
                     RALDLHLQEIFSETLRLMYPENPSKHLDAYLGCDEWSLFSEVRGWLSVKDARRKKLAL
                     EWRKIFARRLKWKMSFAAEISVDSMQRGTEFVGAQNYEARVRECLPASLQRLPFRVDV
                     ATQDPRPLNPIAEPGKRINIFNPVTGKTSPEPLNEIYRFIPARVRHFRVFALNHDHDE
                     ELARAAESALGNIERSIPTNV"
     gene            complement(1735..2499)
                     /gene="ubiE"
                     /locus_tag="PLQ77_07725"
     CDS             complement(1735..2499)
                     /gene="ubiE"
                     /locus_tag="PLQ77_07725"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPL10604.1"
                     /translation="MNTNVPEKYPRVQEISDAQRIRIVKEIFSTVTGKYDFLNRLLSL
                     RRDVAWRKFAAKKMRFFQTRRYLDVACGTGDLSIAAAHMHPDISVTGLDFVNEMVAAA
                     KDKVQKKNLAGRIQIMEGDALHLPFEDNRFDVAGIAFGIRNIPDRARALSEMLRVVVP
                     GGQVMILEMTFVQNRFFKGIYYVYLNFLLPAFAKIFAKNPAAYSYLADSIMNFPSPGQ
                     FAKILEEAGMVHVEKYSLTFGITWLHVGRKAMGNRL"
     gene            complement(2643..3140)
                     /locus_tag="PLQ77_07730"
     CDS             complement(2643..3140)
                     /locus_tag="PLQ77_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL10605.1"
                     /translation="MMRIKNLLPTIARPAARDDDHPFYSLQRQMNSLFDDFLAGFEAA
                     PRALSAGGFGAFIPSVDVKESDKDFVIRAELPGVEEKDIEVTITGDSVTIKGEKKEEK
                     EDKGKNYYYMERSYGSFNRVIPLAGETDANKASAGFKNGVLNICIPKIQSAKAKGTKV
                     PIKAG"
     gene            complement(3402..3566)
                     /locus_tag="PLQ77_07735"
     CDS             complement(3402..3566)
                     /locus_tag="PLQ77_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HPL10606.1"
                     /translation="MYSEYEIGDYERVFTLSDRIDRDKIVATVKNGVLRLELPKAEEV
                     KPKKIAIKAA"
BASE COUNT          737 a         1011 c          962 g          867 t
ORIGIN      
        1 taacgatatg tagggcgggt cttcaggccc gccattgccg ggtctaaaga cccgggctac
       61 attgtttggg tgagatagcg atgtttatgt agggcgggcc ttcaggcccg ccaacccaag
      121 acaggctcgt gaaaaagcag gttgtcaccc tgagcccgtc gaagggcatt acctgtcttt
      181 tcactcatca ctcatcactt tccccgtctt ccccaattac cgaattgatc tttgaggcat
      241 aatattgtaa atgctcacca tggacaagag agatttcccc tccaacgtgt atgacggaat
      301 ggcgctgata gccgatccga tccactccta tgcgtttttc acggcgcccc gggagtccga
      361 ccccgcggaa aaaacggaaa aggacatcat cgactctccc tggatgcaga ggcttcggcg
      421 catttaccag ctgcagagcg cccgctgggt ttatccggcg gcggagcaca cccgttttca
      481 gcattcgctg ggcaccatgc atgttgccgg agaatttgcg aggcatcttt atcccagcct
      541 caggcaggtg tgccgggatg cgccgtcgat gcattacgtc gaggagctgc tccggctcgc
      601 gggtctgctg cacgacgtgg ggcacggacc cttcggccat ttttttgatg agcattatct
      661 ttccggctgg gcgctgaccc acgaggacat gggccggctg atcatcgtga agaagctggg
      721 gccgctcctt gcgaaaattt cccgcagtcc ttccgggcct ttcgcccgcg gggaaaaact
      781 cgatccgcgg caggtggcct tcctgatcaa aatgccggaa gaagaggagc cccgtcagcc
      841 gcgctggctg gtctggctgc gccagttgtt ttccggcatc tatacggtcg ataatctgga
      901 ctatgttcag cgcgacgcct acatgaccgg cttttccctg gacatggtcg atgtgccgcg
      961 cctgcgctac tacacgtttt ttacgaaaga cggcctggcg ctccatcagt cgggcatttc
     1021 ggcgctccgg cgctttctca acgcccgcct gaatctctac agcaacgtgt attttcaccg
     1081 cacgacgcgc gcgctggatc tgcacctgca ggaaattttt tccgaaacgc tgcgcctgat
     1141 gtatccggaa aatccatcaa agcatcttga cgcatacctg ggctgcgacg agtggtcgct
     1201 gttcagcgag gtgcggggct ggctttccgt caaagacgcg cgccgcaaaa aactggctct
     1261 ggagtggcgg aagatttttg caaggcggct gaagtggaag atgtcttttg ccgccgaaat
     1321 ctccgtcgat tcgatgcagc gggggacgga gtttgtcggc gcccaaaact atgaggcgag
     1381 agttcgggaa tgcctgcccg cctcgcttca gcgcctgccg tttcgcgtgg acgttgccac
     1441 gcaggacccg cgtccgctca atccgatcgc cgaaccgggc aaacgcatca atatcttcaa
     1501 tcccgtcacg ggcaaaacgt cacccgaacc gctcaatgaa atctaccggt tcattccggc
     1561 ccgcgtccgg cacttccgcg tgttcgcgct gaatcatgat catgacgagg agctggcccg
     1621 ggcggccgaa tcggcgctgg gcaacatcga acgctccatt ccgacgaatg tgtgagaaac
     1681 aggcgagggg ccaggggcga taggccatag gcaacaaatc ctatagccca tagcctatag
     1741 cctattgccc attgcctttc ttcccacatg cagccaggtg atgccgaagg tcagggaata
     1801 tttttccaca tggaccatgc ccgcttcttc gaggattttt gcaaattgtc cgggtgacgg
     1861 gaagttcatg atggagtcgg ccaggtagga gtaggcggcg ggatttttgg caaagatttt
     1921 tgcaaacgcg ggcagcagaa aattcagata gacatagtag atccctttga aaaaacggtt
     1981 ctggacaaag gtcatttcca ggatcatgac ctgcccgccg gggacgacga cgcgaagcat
     2041 ttcggaaagc gcgcgggcgc ggtcgggaat gttgcggatg ccgaaggcga tgcccgcgac
     2101 gtcgaagcgg ttgtcttcaa aaggcagatg aagggcgtcg ccttccatga tttgaatccg
     2161 tccggcaagg tttttcttct gaaccttatc tttggccgcc gccaccatct cgttgacaaa
     2221 gtcaaggccg gtgacggaaa tatccggatg catgtgggcc gccgcaattg aaagatcccc
     2281 cgttccgcag gcgacgtcca gataccgccg tgtttgaaag aaccgcattt tttttgcggc
     2341 gaatttgcgc caggccacgt cgcgccgaag gctcagcaga cggtttaaaa agtcgtattt
     2401 tccggtcacg gtggagaaaa tctctttgac gatgcggatg cgctgggcgt cggagatttc
     2461 ctgaacgcgc ggatattttt cggggacgtt cgtattcata aaacaggttc ctgtcgtttt
     2521 tcctgccgcg gatttagcag tccgggcggg gaaatgcaac aagaaagcaa cgagtaaaaa
     2581 aaccgccgcc cgtcaggtga agggcggcgg taatccatgt tgtcttgaac ttcagccttc
     2641 ttctaccccg ctttgatggg aaccttggtc cctttggctt tagccgactg gattttcgga
     2701 atgcaaatgt tcaggacgcc gtttttaaag ccggcggacg ccttgttcgc gtcggtttcc
     2761 ccggccagag ggatcacgcg gttgaaagag ccgtaagagc gctccatgta gtaataattt
     2821 ttccccttgt cttccttttc ttccttcttt tcgcctttga ttgttactga atcgccggtg
     2881 atcgttactt caatatcttt ttcctccacg ccgggaagct ccgcgcggat cacaaaatcc
     2941 ttgtcgcttt ccttgacgtc caccgacggg atgaacgctc cgaatcctcc ggcggaaagc
     3001 gcgcgcggcg cggcttcaaa tccggcaagg aaatcatcaa acaaactatt catttggcgc
     3061 tgcaaagaat agaagggatg atcgtcatct cttgccgcag gccttgcaat ggtgggcagc
     3121 agatttttta ttctcatcat catatcctcc tttcttttgg atctaaaggg tcggcgtcgg
     3181 gcactgtcat ggttcgacaa gctcaccact cgacaggctc atcatgacac gctgtattgt
     3241 taccctaatc aaatcgaaag gtatgagcca tgagcttttc ccttattacc cattacctct
     3301 gacctatggc ccctcgcctc tcttttcact catcactttc cgcttttcac ttcttgccta
     3361 tcgcccatgg cctctcgcct gtttgtttac catccactac gttatgccgc cttgatcgca
     3421 atctttttcg gtttcacttc ttccgctttc ggcagctcca gtctcagaac gccgttcttg
     3481 acggtggcga cgattttatc ccggtcgatc cggtccgaca gggtgaacac cctttcatag
     3541 tcgccgattt catactcgga gtacatcatc gccgcgt
//
LOCUS       DAOPAF010000104         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_21462_length_3518_cov_38.822408, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000104 DAOPAF010000000
VERSION     DAOPAF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21462_length_3518_cov_38.822408"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1399)
                     /gene="dnaB"
                     /locus_tag="PLQ77_07740"
     CDS             complement(41..1399)
                     /gene="dnaB"
                     /locus_tag="PLQ77_07740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPL10607.1"
                     /translation="MKDIDPSLYKLPPQNIEAEQSILGSILLENGTINAVQEILNRDD
                     FYSEAHRKIFSAIERMADKNEPVDLITLSSALKDQNLLDSIGGAAYLASLVDNVPTAA
                     NAANYAKIVKGKSVLRGLIGSATEIINSCYDTGSDVDQVLDIAEHRIFEISENKVRAS
                     FYPIKDIVRDTFRSIEDLFTRKELITGVPTGFDKIDDMTSGLQNSDLIIVAGRPSMGK
                     TALALNVAQHAALETQIPVAIFSLEMSKEQLAFRMLASEARVDSQRMRKGFLGETDWP
                     KLTAAAGRLSEAPLFIDDTPAITVLEMKAKSRRLKAERDLGLIVVDYIQLMRSSGNTN
                     SREQEISEISRSLKALAKELKVPVIALSQLNRQVETRPGGNKRPQMSDLRESGAIEQD
                     ADVIIFIYRDEVYNKSDDNPEKGTAEIIIGKQRNGPTGPVKLAFLDRFTTFENLARTD
                     EP"
     gene            complement(1396..1845)
                     /gene="rplI"
                     /locus_tag="PLQ77_07745"
     CDS             complement(1396..1845)
                     /gene="rplI"
                     /locus_tag="PLQ77_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPL10608.1"
                     /translation="MKVILKQNVPSLGKAGDLVKVNDGYARNLLIPKGLAVEADDKNL
                     KAFELAKKNILQKTQKEKAGAQDLASRLSQVTLTLSRKVGDQHKIFGSVTSKDLEAAI
                     REKGFPVDRKMIVHDEPIKSLGEFKVRIKLHPGVETEIQVHVVGEES"
     gene            complement(1880..2845)
                     /locus_tag="PLQ77_07750"
     CDS             complement(1880..2845)
                     /locus_tag="PLQ77_07750"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPL10609.1"
                     /translation="MTTLNLSPFHDASFPRLLLVIALFLAASLLPFAGLVFLLVIPLV
                     LYVLCFLSDPAKTLKAFLASLCAVAALLWTMNAAASVAAPAAMGIAGMLMAWSAEKRF
                     RVEAVVLLPALVVLGAIAFYFIFGALQQSLTPWELVLKNIRESVELNIEFYSRLPLNP
                     EELQAIVDSKPQMISLFARIFPALCVMAAVFTIWVNLIAAGRLLKSRGIVLPQLSNLS
                     QWSAPGWLVWVFIAAGGLGAVPVTPIRVVGVNILLVASLLYFLQGLAIVSFFFQHKNI
                     SPFFRFLAYFLIAIQQILMIAIAAVGFFNLWVDFRKYLRKDRSTD"
     gene            complement(2963..3232)
                     /gene="rpsR"
                     /locus_tag="PLQ77_07755"
     CDS             complement(2963..3232)
                     /gene="rpsR"
                     /locus_tag="PLQ77_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPL10610.1"
                     /translation="MAAPNGERPSRPPQKKFFHRKKFCRFCTDSNVKIDYKNPVILNN
                     FVTERGKIMPRRITGNCAYHQRELTVAIKRARTIAIMPFVTAEGR"
     gene            complement(3235..>3518)
                     /locus_tag="PLQ77_07760"
     CDS             complement(3235..>3518)
                     /locus_tag="PLQ77_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10611.1"
                     /translation="DYAGDGSIVAEMERNFKIDDRILKFMTVTREGAVTREGIDAEIA
                     ASEVKRTQIRIETDNTAAAKEEKAAPDAKPSRLSFRKAQPREDNTPKGE"
BASE COUNT          755 a          941 c         1014 g          808 t
ORIGIN      
        1 tgcatccggt cgcaaacgat cacttctacg ggatttcttt tcatggctcg tccgtccggg
       61 ccaggttctc aaaggtggtg aaccggtcca ggaacgcaag cttcaccgga ccggtggggc
      121 cgttgcgctg cttgccgatg atgatttcgg cggtgccttt ttccgggttg tcatcggatt
      181 tgttgtacac ctcatcccgg tagatgaaga tgatcacgtc cgcgtcctgt tcgatggcgc
      241 cggattcgcg caggtccgac atctgaggac gcttgttgcc gcccggcctt gtctcgacct
      301 ggcggttgag ctgggaaagc gcgatgacgg gcaccttgag ctccttggcc agcgccttga
      361 gcgagcggct gatctcggaa atttcctgct cgcgcgaatt ggtattgccg ctggaccgca
      421 tcaactgaat gtagtccacg acgatcagcc ccaggtcccg ctccgctttc aggcggcggg
      481 actttgcctt catctccaaa acggtgatgg cgggcgtgtc gtcgataaac agcggcgctt
      541 cggagagccg gcccgccgcc gccgtcagct tcggccagtc ggtttccccc agaaaaccct
      601 tgcgcatgcg ctgagagtca acacgggctt cggacgccag cattctgaag gccagttgct
      661 ccttggacat ttccagcgag aaaatggcaa caggaatctg ggtttccaga gcggcgtgct
      721 gtgcgacatt gagcgctaag gcggtcttgc ccatgctggg gcggccggcg acaataatca
      781 gatcggaatt ctgcagcccg gaggtcatgt cgtcgatttt gtcgaagccg gtgggaacgc
      841 cggtgatcag ctccttgcgg gtgaacaggt cctcgatgga gcggaaggtg tcgcggacga
      901 tgtccttgat cgggtaaaaa gaggcccgca ccttgttttc ggaaatttcg aaaatgcggt
      961 gctcggcgat atccagcacc tggtcgacgt cggagcccgt gtcatagcaa ctgttgatga
     1021 tttcggtggc ggacccgatc aggccgcgca gaaccgactt tcccttgacg atcttcgcgt
     1081 aattggccgc gttggcggcc gtgggaacgt tgtccaccag cgatgccagg taggccgcgc
     1141 cgcctatcga atccagcaaa ttctggtcct tcagcgcgct gctgagcgta atcaggtcca
     1201 ccggctcatt cttgtccgcc attcgctcga ttgcggaaaa aattttccgg tgagcttcgc
     1261 tgtagaagtc atccctgttc aggatttcct gcaccgcgtt gatggtgccg ttttccagca
     1321 gaatgctgcc caggatggac tgttcggcct cgatattctg gggcggcagt ttgtatagag
     1381 agggatcaat gtctttcatg attcttcgcc gaccacatgg acctggattt ccgtttcaac
     1441 gcccgggtgg agcttgatgc gaaccttgaa ctctcccagt gatttgatcg gctcatcgtg
     1501 cacaatcatt ttgcggtcca caggaaatcc cttttccctg atcgccgctt ccaggtcctt
     1561 ggatgtaacg gagccgaaaa tcttgtgctg atctcccact ttgcgggaaa gcgtcagcgt
     1621 cacctggctc agacgggaag cgagatcctg cgcgccggcc ttttcctttt gcgtcttttg
     1681 cagaatattt tttttggcca gttcaaaggc cttgagattc ttgtcgtccg cctcaacggc
     1741 cagacccttc ggaatcagca gattgcgggc gtagccgtcg ttgaccttga ccaggtctcc
     1801 ggccttgccc agagaaggga cgttttgctt caaaatcacc ttcatggtca aacctcctgt
     1861 ctcgtatgga atgcaatggt cagtctgtcg accggtcttt gcgcagatat tttcgaaaat
     1921 caacccagag attaaagaat cccactgccg ctattgctat cataagaatt tgctgaatcg
     1981 caattaaaaa ataagcaagg aagcggaaaa acggtgagat gtttttatgc tgaaaaaaga
     2041 agctaacaat agcaagacct tgcagaaaat atagcaaaga cgcgacgaga aggatgttga
     2101 cgccgacgac gcggatgggg gtgacgggga cggcgccgag ccctcccgcc gcgatgaaaa
     2161 cccagaccag ccagccgggc gcgctccatt gggacaggtt cgaaagctga ggcagtacaa
     2221 tgccgcggct tttgagcagc ctgcccgcgg ctatcagatt cacccagatc gtgaatacgg
     2281 ccgccatgac gcaaagggcg gggaaaatcc gcgcaaacag ggatatcatt tggggcttgc
     2341 tgtccacaat cgcttgaagt tcttcgggat tcagaggcaa tcgactgtaa aactcgatgt
     2401 ttaattccac cgattcccga atgttcttta aaaccagctc ccagggggtg agcgactgct
     2461 gcaacgcgcc gaaaataaaa taaaaggcga ttgcgccaag cacgacgagc gcgggcagaa
     2521 gtacaacggc ttcgacacga aaccgttttt cggcgctcca ggccatcagc attcccgcga
     2581 tccccatggc cgcaggggcc gcgaccgatg cggccgcatt catggtccac aggagggcgg
     2641 ccacggcgca aagcgaggcc agaaatgcct tgagggtctt tgcgggatcg gaaagaaagc
     2701 acaggacata cagaaccagc ggaatcacga gcaggaaaac gaggccggca aaggggagca
     2761 gcgatgccgc cagaaaaagc gctatgacca gcagcagccg gggaaacgaa gcatcatgaa
     2821 aaggcgatag attcaatgtt gtcaaatcag gggcctgcgg tgaaaaaacg gcaatgccgt
     2881 caaaggatgt gtcggatcaa tgaaaaccgg ccggtttttt cttcatcaga ataaaaccgg
     2941 ccggtggctt tgcaaaattg gtttatctgc cttccgcggt gacaaaaggc atgatggcga
     3001 tggtgcgcgc tcttttaatg gcaacggtca gttctctctg atgataagcg cagttgccag
     3061 tgattctccg gggcatgatc ttgccgcgct ccgtgacaaa attattgaga atcaccggat
     3121 ttttataatc aatcttcaca ttggagtccg tgcagaaccg gcagaatttt ttccggtgga
     3181 aaaacttttt ctgaggcgga cgggagggtc tttcgccgtt gggtgcagcc atgatcattc
     3241 tcctttcggt gtattgtctt cccggggctg ggctttgcgg aaggacagcc gggagggctt
     3301 tgcatccggg gccgcctttt cttctttcgc ggcggctgtg ttgtccgttt cgatgcggat
     3361 ctgtgtgcgc ttcacttccg aggcggcgat ttccgcgtcg atgccttcgc gggttacggc
     3421 gccttccctg gtgaccgtca tgaatttcag aatccggtcg tcaattttaa aatttctctc
     3481 catttcggcc acgatggaac cgtcgccggc atagtcga
//
LOCUS       DAOPAF010000105         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_21881_length_3459_cov_44.405112, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000105 DAOPAF010000000
VERSION     DAOPAF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21881_length_3459_cov_44.405112"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PLQ77_07765"
     CDS             complement(<1..994)
                     /locus_tag="PLQ77_07765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPL10612.1"
                     /translation="MAEQTNQGRVLSVRSSVVDIHFPVAPPIRNVLTAGEKGEVVIEV
                     FTQLDNNTIRGVALTPTQGLARGAVVTDTGKPFEVPVGSQLLGRVFNVFGNTIDKKGN
                     VEGCEARSIHREPIPLRDQSTVSEIFETGIKAIDVLAPLERGGKAGLFGGAGVGKTVL
                     ITEMIHNTVSGHEGMSIFCGIGERCREGEELYREMEESGVLGNTVMVFGQMNEPPGAR
                     FRVGHAALTMAEYFRDDAKQDVLLMIDNIFRFIQAGMEVSGLLGQLPSRLGYQPTLAT
                     ELAELEERICNTKTGAITSIQAVYVPADDFTDPSAVHTFGHLSATIALSRKRASE"
     gene            complement(1011..1466)
                     /locus_tag="PLQ77_07770"
     CDS             complement(1011..1466)
                     /locus_tag="PLQ77_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10613.1"
                     /translation="MLASLKLLDVMANNSEAIARNWAKDVAQNAKTPSYHDLHENHLI
                     PQAADFYRKLSSVFTLKDPYPAIYDFMSKFAEKRFREEVPLQEVLYAIILMRRHIWLY
                     AEFQAIFVTITEQQQALESQTRTILIFDYIAYILTDKYMALMGAAPKKK"
     gene            complement(1872..2567)
                     /locus_tag="PLQ77_07775"
     CDS             complement(1872..2567)
                     /locus_tag="PLQ77_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPL10614.1"
                     /translation="MKINQLPAPVLTYGLVPLAHRLILLHTFMIRTEVVDEEVITSRL
                     DRGEKGIVAIWHQRMYGVIGYAKAASRYRPSAIISRSGDGDLISELVRRLHFRPIRGS
                     SSRGGKEALAAIVEDLKENLLAIHAVDGPRGPGGTVKAGLIRMAELSGAKIFPVYVSF
                     SRAWPLRSWDRFLIPKPFSRVRIRWDQPISVPANMKEQEFESIRRSLEIHMRQNQITD
                     DRSFGQESLLLEP"
     gene            2724..>3459
                     /locus_tag="PLQ77_07780"
     CDS             2724..>3459
                     /locus_tag="PLQ77_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPL10615.1"
                     /translation="MIRRIIFALAAAALLIHFIFALIEAGKGIGGAKAAPTIFYGQPL
                     EIRKGDHPGNLRLTERLNRLSYRQVREKPAKSGTFSLTKERLWLFLRGAHEKGFLQSS
                     GPVEIALANGRVASLASPDGKPLDSVRLEPEEIGRLAGPKMESRTTVSLSGVSPYLQN
                     AVIACEDKRFYHHIGIDPLAAGRALSVNLREKRFAQGASTITQQLARNVFLSPKKTFA
                     RKLREMELALALELRYSKQQILEMYLN"
BASE COUNT          741 a          984 c          989 g          745 t
ORIGIN      
        1 cttcactggc gcgcttgcgc gaaagcgcga tggtcgcgga caggtgaccg aaggtgtgca
       61 cggccgaggg atcggtgaaa tcgtccgccg gaacataaac ggcctgaatg gaggtgattg
      121 cgccggtctt ggtgttgcag atgcgctcct cgagctccgc gagctccgtg gccagcgtcg
      181 gctgataccc gagccgggac ggcagctgcc ccagaaggcc cgacacttcc atgcccgcct
      241 ggatgaagcg gaagatgttg tcgatcatca gaagcacgtc ctgtttggca tcgtcgcgga
      301 aatactcggc catggtcagc gccgcgtgtc cgacgcgaaa ccgcgcgccc ggcggttcgt
      361 tcatctggcc gaacaccatg acggtgttcc ccagaacgcc gctctcctcc atttcccggt
      421 agagctcctc gccttcgcgg cagcgctcgc cgattccgca gaagatgctc atgccttcgt
      481 gaccgctgac ggtgttgtga atcatctccg tgatcaaaac ggtcttgccg acgccggcgc
      541 cgccgaacaa cccggccttg ccgcctcgtt ccaaaggcgc caggacgtcg atggccttga
      601 tgccggtttc aaagatttcc gagacggtgg actgatcgcg cagcggaata ggctcgcggt
      661 gaatcgatcg ggcctcgcac ccctcaacat tacccttctt gtcgatggtg ttgccgaaga
      721 cattgaacac ccttcccagc aactgactcc ccacgggaac ctcaaagggt ttgccggtat
      781 cggtgaccac ggccccgcgg gccagaccct gcgtcggcgt cagggcgacg ccccggatgg
      841 tattgttgtc cagttgtgta aacacctcga tgacgacctc acccttctct cccgccgtca
      901 gcacattgcg gatcggcggg gcaacgggaa agtggatgtc aacgacgctg ctgcgaacgg
      961 ataaaaccct tccttgattt gtttgctcgg ccacggttcg ttcacctcgt ttattttttc
     1021 ttgggagcgg ctcccattaa tgccatgtat ttatccgtca ggatataagc gatgtaatcg
     1081 aaaataagaa tcgtccgcgt ctgactttcc agggcctgct gctgctcggt aatggtaacg
     1141 aagatggcct gaaactcggc gtaaagccaa atgtggcggc gcatgagaat gatggcgtaa
     1201 agcacctcct gcagcggaac ttcttccctg aaacgttttt cggcaaattt ggacatgaag
     1261 tcgtaaatgg cggggtaagg atctttcagg gtaaagacac tgcttaactt gcggtaaaaa
     1321 tccgcagcct gaggaataag atgattttca tggagatcgt gataggaagg cgtcttggcg
     1381 ttttgggcga cgtctttcgc ccagtttcgt gcaatcgcct cggaattgtt tgccatcaca
     1441 tccagtagct tcaacgatgc cagcatatgc cttctccctg cttctttggg aaacctgccg
     1501 gtttccccat gaggatgctg catgactgcg gatcaaagag cggactgcct cggtttcccc
     1561 caaccaatga gcaatcggcg tgccagaaat gtcattttac gcaaaccatt gattttgctg
     1621 atggtgcgaa gaccatgcca ttgaacgccg tcggaacggg cgacccgaat gtcttcggtt
     1681 tgatcgcgca ccgtcaaggg cacacccgga aaatgcagac cggtgcaggt tttgagcagg
     1741 atgtcttatt tgtcacgggg gcgcgacgta aatgtcgcgt tcgggaaaat ccgcggcctc
     1801 atgattccgt ctgtgaggaa aaattgcgaa aagcagatca acgaaaagga cggcagcccc
     1861 gtgaaacaaa cctatggttc aaggagcaga ctctcttgac cgaaagaccg gtcgtcggtg
     1921 atctggtttt gccgcatgtg aatttccagc gaccggcgga tggattcgaa ctcctgttcc
     1981 ttcatgtttg ccggaacgga gatgggctgg tcccagcgga tccggacccg gctgaaaggt
     2041 ttgggaatca gaaaccggtc ccagcttcgc aaaggccagg ctcggctgaa ggagacgtaa
     2101 accggaaaga ttttcgcgcc ggaaagctcg gccatgcgga tcaatccggc tttgaccgtg
     2161 ccgccgggtc cacggggccc atccaccgca tggatagcga gaagattttc tttcaggtcc
     2221 tccacgatgg cggcgagcgc ctctttccct cccctggaac tggagccccg aatggggcgg
     2281 aaatggaggc gacggaccag ctcgctgatc agatcgccgt cgccgcttcg gctgatgatg
     2341 gccgaaggcc ggtatcggct agcggccttg gcatagccga tgactccgta catgcgctga
     2401 tgccagatcg ccacaattcc cttttctccc cggtccagac gggaggtaat cacctcttca
     2461 tcgaccacct ccgtgcgaat cataaaggtg tgaagcagga tcaggcggtg agccaggggc
     2521 acaaggccgt acgtgagaac cggcgcggga agctgattga tcttcatgga tcatgcatat
     2581 ccgcaattgc atttgccgtc aacgcggaaa tcgcaagggg cttaaattat cttgctatcc
     2641 ggccgtgtta tccgtaagat tcggccctga aaaaacgatt tgccgtccat cgactttatc
     2701 tgaaaagagg ctgggaggat tcattgattc gtcgaatcat ttttgcgctg gcggcggccg
     2761 ctttgctgat tcatttcatc tttgcgctga tcgaggcggg caaaggcatc ggcggcgcca
     2821 aggcggcgcc cacgatcttt tacggacagc cgctggaaat ccggaaaggc gatcacccgg
     2881 gaaatctccg cctgaccgag cggctgaatc ggctttccta ccggcaagtt cgggaaaaac
     2941 cggcaaaatc cgggaccttt tcactgacca aggagcgcct gtggctgttt ctgcgcggcg
     3001 cgcacgaaaa aggatttttg cagtcttccg gacctgtgga gatcgccctc gccaacggac
     3061 gggtagcttc gcttgcgtcc ccggacggca aaccgctgga ttcggttcgc ctggagccgg
     3121 aggaaatcgg ccgcctcgcg ggccccaaaa tggaatcccg aacgacggtt tctctttccg
     3181 gcgtttcccc ttacctgcaa aacgccgtca tcgcctgcga agacaagcgc ttttatcatc
     3241 atatcggaat cgatcctctt gccgccggac gggccttatc ggtcaacctt cgggagaagc
     3301 gatttgccca gggcgcatcc acgataacgc agcaactggc aagaaacgtc ttcctttctc
     3361 cgaaaaaaac tttcgcgcgc aaactgcggg agatggaact cgcactggcg ctggagctgc
     3421 gctactcgaa acaacagatt ctggaaatgt atctgaaca
//
LOCUS       DAOPAF010000106         3406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_22279_length_3406_cov_53.719785, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000106 DAOPAF010000000
VERSION     DAOPAF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22279_length_3406_cov_53.719785"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PLQ77_07785"
     CDS             <1..1664
                     /locus_tag="PLQ77_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPL10616.1"
                     /translation="AFTWPTTAKSFFHQMFFEVWLANVPILTACCLLAFAVNGLYRGT
                     RNVPYKGRLVIVVRGVGTAFLLFLLWIYLTEAFIPRSTMAAGWFLIFVLILSSRLIST
                     AFYRQYHVIPADAYDPRIEQIVRELTLISHQDGWLPPESLPPKAAWPHFDEDEVLAVA
                     AVLNSGKINQWTGREVERFQEEFASFCGVKHAVALANGSVALDLALRALNIGPGDEVI
                     VTPRTFVVSAGCVAMRGAKPVFVDVDRESQNMSAEAVRAAIGPRTRAIIAVHLAGWPC
                     EMDPILDIAEAHGLKVIEDCAQCHGAVYYSRRPGNGSAGLGSSIERDGVLLYPRMTGS
                     MGDMAAFSFCQDKIMTTGGEGGMLLMNDDGLWEKAWAFKDHGKSYDAVYRREHPPGFR
                     WLHESFGTNWRMTEMQAAIGRRQLEKLPGWVEARRRNAAILTEAFGAIPGLRVTPEPE
                     NVRHAYYKYYAFVRPERLKAGWDRDRIMNAVTAAGAPCFSGSCSEVYLEKAFEGNGLR
                     PAERLPVAKELGETSLMFLVHPTLTENDMKRVAAIVAAVMAEATE"
     gene            2001..3389
                     /locus_tag="PLQ77_07790"
     CDS             2001..3389
                     /locus_tag="PLQ77_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL10617.1"
                     /translation="MKFVNRHSELTFLKERHEQDGFQFIPIYGRRRVGKTRLLREFLR
                     DRPGIYFMADTAPEREQLKSLGREVGEFFQDNILAASGFQDWQQVFAYLKEKSRHTRL
                     VLALDEFPYLVGANSAISSIFQKGVDLDLQDSRLMLLLTGSSMGMMEEEVLHARAPLY
                     GRRTGSLEVKEMPFETLAELFPDMPFAGRVSIYSLFGAIPAYLERVIPTLTPLENIRR
                     SVLDRGSFLYSEVEFLLREELREPRNYFVILRAIAQGKQKLSEIINDTGMDKSHVSRY
                     LDILRSLRLVEKEVPVTEKYPDKSRAGLYRLHDRFFTFWFRYVFPFRGRLEIDQVDNV
                     LEMIAETFDTFVGNAYEDICRAQCLSLMKKGVMAFTTLGRWWERNEEIDIVALDETEN
                     VAWFGECKWSRKKVGTDIYEDLVRKSRLVSWRDSTRFDRYVLFSRSGFTEAMVAKAKE
                     EGVVLIHGDSIA"
BASE COUNT          716 a          831 c         1089 g          770 t
ORIGIN      
        1 aggcgttcac ctggccgacg actgcaaaat ccttcttcca tcagatgttt tttgaggtct
       61 ggctggccaa tgtacccatc ctcacggcct gctgtctgct tgcatttgcg gtgaacggac
      121 tttaccgggg gacgcggaac gtgccctaca aggggcgact cgtgatcgtc gtgcgtgggg
      181 tgggaactgc ctttcttctt tttcttctct ggatttatct gacggaggcg tttattcccc
      241 gcagtacgat ggcggcggga tggtttctca ttttcgttct tattctttcc agccgtttga
      301 tttcgacggc tttttaccgt cagtatcatg tgattcccgc ggatgcctac gatccccgga
      361 ttgaacagat cgtccgggag ctgacgctga tttcccacca ggacggctgg cttccgccgg
      421 aaagcctgcc cccgaaggcc gcatggccgc atttcgacga agatgaagtt ctggctgtcg
      481 ccgcagtgct gaattccggg aagatcaacc agtggacggg cagagaggtg gagcgcttcc
      541 aggaggaatt tgcgtcgttc tgcggggtga agcatgccgt ggcgctggcg aacggttcgg
      601 tggccctgga tctggcgctc cgtgcgttga acatcgggcc gggggatgag gtgatcgtga
      661 cgccgcggac gtttgtcgtg tcggcgggtt gcgtggccat gcggggagcg aagcccgtgt
      721 ttgtggatgt ggaccgggaa agccagaata tgagcgccga agcggtgcgg gcggcgattg
      781 gcccccggac gcgggcgatc attgccgtgc atctggcggg atggccgtgt gagatggatc
      841 cgattctgga tattgccgag gcgcacgggc tgaaggtgat cgaggactgc gcgcagtgcc
      901 acggggcggt ttattacagc cgacggccgg ggaacgggtc ggcggggttg ggatcatcca
      961 tcgaacggga tggcgtgctt ctttacccgc gcatgacggg cagcatgggg gacatggcgg
     1021 ccttttcctt ctgccaggac aagattatga cgacgggcgg cgaaggcggc atgttgctga
     1081 tgaatgacga cggcctctgg gagaaggcct gggcgttcaa agatcacggc aagagctacg
     1141 acgcggtcta tcggcgagag catccgccgg gattccgttg gctgcacgaa tctttcggga
     1201 cgaactggcg gatgacggag atgcaggcgg caatcggacg gcggcagttg gagaaactgc
     1261 cgggctgggt ggaggcgcga cggcgaaatg cggcgatttt gacggaggcg tttggcgcga
     1321 ttccggggct gcgggtgacg ccggaaccgg agaacgtgcg gcacgcctat tacaagtact
     1381 acgcctttgt ccggccggag cggctgaagg cgggctggga cagggaccgg atcatgaacg
     1441 cggtaacggc ggcgggcgcg ccctgcttca gcgggagctg cagcgaggtg tatctggaaa
     1501 aggccttcga gggcaacggc ctgcgtccgg cggagcgcct gcccgtggcc aaggaattgg
     1561 gggagacgag cctgatgttt ctcgtccacc ccacactgac ggaaaatgat atgaaacgcg
     1621 tcgccgccat tgtcgcggct gtcatggcgg aggcaacgga gtaaaggggt gataggtaat
     1681 gggttacaga tgtagtgcgg ggttttaacc ccgctaaaaa cagcgcggct aaagccacgc
     1741 actacatcgg gttggaatgt tttaaatgta gtgcggatat ttatgtcctc gaaaaaacag
     1801 gtcatttcca caacctctgt cattcccgta atctctactc ccaccctgtc gagacctttg
     1861 aaaaatgtca tgacaaaaat ggcagttatt caaggctctc gaaggggttg tgcattaaat
     1921 aggagcaact ttgcttgaca gaaagcgttc tttagtctaa ttagaattag actaaagatg
     1981 attagactga agggaatatc ttgaaatttg taaatagaca cagtgagttg acattcctca
     2041 aggaaaggca cgaacaagat ggatttcagt ttattcccat ttatggtcgt cgccgagtgg
     2101 ggaagacccg cctgctccgc gagtttctca gagacaggcc cggtatctat ttcatggcgg
     2161 atacggcccc ggagcgggag caactgaaaa gcctggggcg ggaggtgggc gagttctttc
     2221 aggacaacat cctggccgca tcagggttcc aggactggca gcaggttttt gcttacctga
     2281 aggaaaaaag ccggcatacc aggctggttc tggcattgga cgaatttccc tatctggtcg
     2341 gcgccaattc cgccatttcc tcgattttcc agaaaggtgt ggatctcgat ctgcaggaca
     2401 gccgcctgat gctgctcctg acgggatcga gcatgggcat gatggaagag gaggtgcttc
     2461 atgccagggc gcccttgtat ggccgtcgca ccggttctct ggaagtgaag gaaatgccct
     2521 tcgaaactct ggccgaactg tttcccgata tgccctttgc gggccgcgtt tctatttaca
     2581 gcctctttgg ggctattccc gcgtacctgg agagggtcat tcccacgttg acgccgctgg
     2641 aaaatatccg ccgatccgtc ctggataggg gttcttttct ctatagcgag gtggagttcc
     2701 tgctccggga ggagctgcgg gagccccgaa attattttgt cattctccgt gccattgccc
     2761 agggcaaaca gaaactatcc gaaatcatca acgatacggg gatggacaag tctcatgtct
     2821 cccgttacct cgacattctg aggagcctga gattggtgga aaaggaagtc ccggtgactg
     2881 aaaaataccc ggacaaaagc agggctggac tgtaccggct tcacgaccga tttttcactt
     2941 tctggttccg gtacgtcttt cccttccgcg ggagattgga aatcgaccag gtggataatg
     3001 tcttggaaat gatcgccgag acgttcgata cctttgtcgg caatgcttac gaagatatct
     3061 gccgggcaca gtgcctttcg ctgatgaaaa aaggcgtgat ggcgttcacg actctcggca
     3121 ggtggtggga gcggaacgaa gagattgata ttgttgcact tgacgaaaca gagaacgttg
     3181 catggttcgg ggagtgcaaa tggagccgaa agaaggtggg aaccgacatc tacgaggatc
     3241 tcgtgcgcaa atcccgactg gtttcgtggc gtgattccac gcgtttcgat cgctatgtcc
     3301 ttttcagccg aagcggcttc acagaggcaa tggtcgccaa ggcaaaggaa gaaggggttg
     3361 ttctgattca cggtgattcg attgcctgaa aaaaggggac ggttct
//
LOCUS       DAOPAF010000107         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_22339_length_3399_cov_60.201854, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000107 DAOPAF010000000
VERSION     DAOPAF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22339_length_3399_cov_60.201854"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3227
                     /locus_tag="PLQ77_07795"
     CDS             <1..3227
                     /locus_tag="PLQ77_07795"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPL10618.1"
                     /translation="ISAIIAVMMLFVLQAAVYVQAQQAGSAAAPAQSASPAAGLPQMT
                     PGQAAAYQSLSPAQQQAIQGELGKTGGKVTPQAIDALKGRPEFQSISPADIEKGKQLL
                     EQKEKAQERQGTEKFDRKVPWAEKTVVGTEPAGDSLFDRARKVGNYQDISLKLQLFGS
                     EFFRESAVRVVTDRKDIPVPLKYVVGPGDEVKLLLWGRLNAQYNLTVDRDGKITIPQV
                     GPMFVAGMTFEDMSKYLITQAEQIVGTNIDVSMGALKTISVFVLGDVRRPGAYTIGSF
                     ATITDALVMVGGSSDIGSLRRIQLRRGSKLITTFDLYDLLLKGDKSKDVILQSGDVIF
                     VPVTGPQVGIAGNVKRPAIYELKDRQDLHTLIEYAGGIIPSADTQQIQVERIIRGEKQ
                     IIVDVNDKHLDKAYSVALHDADLVKIFSIVDANVNAIYLSGNVKRPGKYEYKPGMAIK
                     DILKPDELLPETHYDYALIKRLKPPSMEAMLVPFNLGKLLFQQDPANNVALQPQDEIF
                     VFNKWLFKDRPYITIEGEIRGNCAPSLSVGGKPSELTGASIGQSREDRTNLALIKLQA
                     VDTELRVNAESNKEILFRLKKITSEIKIGDAVPTREILEVRDELKGQGKTDLAGRMDS
                     ILEDLKSSCKMYLVDDMKVRDAVLYSGGLTTNSYLDKAEIIRVDDRNKYTFIHFDLKN
                     AMTGVTADNLPLQHKDKIIIHSVQEHAYKKTVAIEGAVSKPGTYQFVEDMRVRDLVFA
                     AGNIMEFAYLDDAEITSMDIEKGASVKLVQKNISLRRALAGDPEHNVALKPYDRVFVK
                     RIMDWRDERFVTIAGQVKFPGRYMLSKGERLSSVIARAGGYTDEAYLRGAVFTREKVR
                     QMQQKSIEEITDRLEKELLSQGAAGVATALSAEEVAAKKAEMEQKKQFIDAMRKSKAM
                     GRMTIKLAHLRLLKGSEYDVEIEDGDTLMIPEKTSVVGVVGAVMSSGAHVYSERKEYK
                     DYIDMSGGYTRYADKDNVYVLKVDGSARKAYNGFVGWNDKKDRHELAAFSDADAYSIE
                     PGDVVAVPENFERVAWLREFRDISQVIANIAFASGNMVLLIRSF"
BASE COUNT          825 a          821 c         1072 g          681 t
ORIGIN      
        1 aaatatcagc aatcatcgcg gtaatgatgt tgtttgtttt gcaggctgct gtttatgtcc
       61 aggcacagca ggcgggaagc gcggcggcgc cggcgcagtc cgcttctccg gcggccggtc
      121 ttccacagat gacgccaggg caggcggcgg cgtatcaaag cctttctcct gcgcagcagc
      181 aggccatcca gggcgaactg ggaaaaacgg gcggcaaagt cactcctcag gcaatcgacg
      241 cgctgaaagg ccgtccggaa ttccagagca tttctccggc ggatatcgaa aagggaaagc
      301 agcttctgga acagaaggaa aaagcccagg aaagacaggg gacggaaaaa tttgacagga
      361 aggttccctg ggcggaaaaa acggttgtcg gcacagagcc ggcgggggac agcctctttg
      421 atcgcgcccg taaggttggg aattatcagg atatttcact taaactccag cttttcggct
      481 ctgaattttt ccgggaatcc gcggttcgcg tggtgacgga ccgcaaagat attcccgtgc
      541 cgctgaagta tgttgtcggc ccgggcgacg aagtcaaact gctgctctgg ggacgcctca
      601 atgcccagta taacctgacc gtggatcgcg acggcaagat tacaattccg caggtggggc
      661 cgatgtttgt cgcgggcatg accttcgagg acatgtccaa atatctgatc acgcaggccg
      721 agcagattgt agggacgaat atcgacgttt ccatgggcgc gctgaagacg atttccgtct
      781 ttgtgctggg ggacgtgcgg cggccggggg cttacacgat cggctctttt gccacgatta
      841 cagacgcgct ggtgatggtg ggcggatcgt cggacatcgg gtccctgcgc cgcattcagc
      901 ttcgccgcgg cagcaagctg attaccacgt tcgacctcta cgatctgctg ctgaagggcg
      961 ataagtccaa ggatgtcatc ctccagtccg gcgacgtgat ttttgtgccg gtgaccgggc
     1021 cccaggtggg aattgccggc aatgtgaagc ggccggccat ctatgagttg aaggaccggc
     1081 aagatctgca cacgctgatt gaatacgcgg gcggcatcat tccgtcggcc gacacgcagc
     1141 agattcaggt ggagcgaatt atccggggcg aaaagcagat cattgtggac gtcaacgata
     1201 agcacctgga caaagcttac agcgtggcgc tgcacgatgc ggatctggtg aagatctttt
     1261 cgatcgtgga tgccaatgtc aatgccattt acttgagcgg caatgtgaag cgcccgggga
     1321 aatacgaata caaacccggg atggcgatca aggacattct caaaccggat gaattgctgc
     1381 cggagacgca ttacgactac gcgctgatca agcgcctgaa gccgccatcc atggaggcca
     1441 tgctggttcc cttcaatctg ggaaaattgc tctttcagca ggatccggcg aacaacgtcg
     1501 cgctgcagcc gcaggacgag atctttgtgt tcaacaagtg gcttttcaag gacaggcctt
     1561 atatcacgat tgaaggcgag atccggggga attgcgcgcc ttcgctcagt gttgggggca
     1621 agccatcgga gctgacgggc gcctccatcg gtcagagtcg ggaggataga acgaatcttg
     1681 cgctgatcaa actacaggca gttgatacgg aactccgggt gaacgcggag agcaacaagg
     1741 agattctatt caggctcaaa aaaattacga gtgaaattaa aatcggcgac gctgtgccga
     1801 ccagagaaat tctggaagtg cgcgacgagc tgaagggcca ggggaaaacc gatctggccg
     1861 gccgcatgga ttccattctg gaggatctta aaagcagttg caagatgtat ctggtggatg
     1921 acatgaaggt gcgcgacgct gtcttgtatt cgggcggcct gaccaccaat tcctatctgg
     1981 acaaggcgga aattatccgc gtcgatgaca ggaataaata tacgtttatc cattttgatc
     2041 tgaagaacgc gatgaccgga gtgaccgcgg acaacctgcc gctgcagcat aaagataaaa
     2101 tcatcattca ttccgttcag gagcatgcct acaaaaaaac ggtggccatc gaaggggccg
     2161 tgtccaagcc cggaacgtat cagtttgtag aggacatgag ggttcgggac ttggtgttcg
     2221 cggcgggcaa catcatggag ttcgcctatc tggatgacgc ggaaatcacg tccatggaca
     2281 tcgaaaaagg ggcgtccgtg aagttggtgc aaaaaaatat cagtttgcgg cgggcgctgg
     2341 cgggcgatcc ggagcataat gtcgcgctga agccgtatga ccgcgttttt gttaagagaa
     2401 tcatggattg gcgcgatgaa agatttgtga ccatcgcagg ccaggtgaag tttcccggcc
     2461 gctatatgct gtcgaagggc gaaaggctgt cttccgtcat tgcgcgggcc ggcggctaca
     2521 cggatgaggc gtatctgcgc ggcgcggtct tcacgcggga aaaagtaagg cagatgcagc
     2581 agaaaagcat cgaggagatc acggatcgcc tggaaaagga gctgctgtcg cagggcgccg
     2641 ccggtgttgc gaccgctctc agcgcggagg aggtcgcggc caaaaaagcc gagatggagc
     2701 agaaaaagca gttcatcgat gcgatgagaa aatccaaggc catgggccga atgacgatca
     2761 aacttgcgca tttgcgcctg ctcaagggca gcgagtatga tgttgaaatc gaggacggcg
     2821 ataccctgat gattccggaa aagaccagtg tggtcggcgt ggtcggcgcc gtgatgagct
     2881 ccggcgcgca tgtgtattcg gagagaaagg aatacaagga ctacatcgac atgagcggag
     2941 gctacacccg ctacgcggac aaggacaacg tttacgtgct gaaggtggac ggcagcgccc
     3001 gcaaggccta caatggattt gtcggctgga acgataaaaa agaccgccac gagctggcgg
     3061 cgttttccga cgcggacgcc tactccatcg aaccgggtga cgttgtcgcc gtgccggaga
     3121 actttgaacg tgtcgcctgg ctgcgggaat tccgcgatat ttctcaggtt atcgccaaca
     3181 tcgccttcgc cagcggcaat atggtgttgc tgatccggag tttctaaaga gtgatgagcg
     3241 atgagtggaa agtgatgagt agggaacggt ttcaaaccgt tccttgtgtc atgggcggta
     3301 gtgtagggaa cgctcgtgag cgttccgtcg agtggaaagt gaaaagtgga aagacaggcg
     3361 aggggcgata ggggaaatct catagcagaa ctggtggat
//
LOCUS       DAOPAF010000108         3329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_22887_length_3329_cov_42.754429, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000108 DAOPAF010000000
VERSION     DAOPAF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22887_length_3329_cov_42.754429"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLQ77_07800"
     CDS             complement(<1..182)
                     /locus_tag="PLQ77_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10619.1"
                     /translation="MRLLKKVLIAVLVFVVLIGLVGFLILPAVLKPVLAKKISEALHR
                     ETAIGQIKINPFDLSAT"
     gene            complement(267..2357)
                     /locus_tag="PLQ77_07805"
     CDS             complement(267..2357)
                     /locus_tag="PLQ77_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPL10620.1"
                     /translation="MEKFFTPRSMVVFGASVTKMNLGQIVLINNKQIGYPGEIYGVGS
                     QEGDINGVHVYTKVADLPCVPDVAIFLTPARTVPGLMEECGRKGITHVVIESGGFSEY
                     SHGHHTLEEDVLAVARKYGMKVIGPNCVGVINFDYKMMMPFAFAKEVPSGGTLGLITQ
                     SGGVGSSYLRTVAGFGITAGKFVSIGNKLQLDEADFLEYLIQDPRTKCIALYLEGFKR
                     GRKFFDLARNCDKPIILQKSNRSPASAKIAQSHTTALSGDDAVVDGAMRQAGVIRVDD
                     EVELVSAVQMVGMPLMKGKRVAVLSRSGGHAVLTADACAKYGFEMTPFPPSFIEKLKT
                     IYNTRVIAHQNPLDLGEIFDYTIFTDILEEALKLDDFDGVLFNHLYSSDFEAEMSRTF
                     LAGVKELVARYEKPVSLAMISDRDEILDLQKKQPFPVFVSPLEAVEALYASRSYYEKK
                     TACQKRGHLEKYDLDLKAVDRIRARCGKEKRIPLTDEALAVSAAAGLQPVKNLLLKDE
                     NIPSRITLRYPLAAKLLSRDASHKSDIGGVAVNLRSKKQLAEALAGMKSKIRNLKPSP
                     SIDGYLIQEMAPAGAECFVGGRRDPAFGPVIVVGLGGIFIEIFKDTSIRMAPVTPGEA
                     EGMIRELKAYPLLAGARGRGPADCQALQNVVCRISALLAACPEISEIDLNPVIVHPEG
                     QGVSLVDARVFFQK"
     gene            2768..>3329
                     /locus_tag="PLQ77_07810"
     CDS             2768..>3329
                     /locus_tag="PLQ77_07810"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPL10621.1"
                     /translation="MKNRRQAQEAPEKDVRQRRPRLQATREPQRPRNLAERELRESEE
                     RLRSVVSRSPIPAFVIGKDHRLLYWNKALEQLTKIRAEDVIGTSQQWRAFYARKRPCM
                     ADLLVSRAQEKISKWYTGKYIRSKLLDEAYEAIDFFPELGHQGKWLRFTAAIIRNEAG
                     DLIGAIETLEDITRRKKAKEALLKAHR"
BASE COUNT          711 a          956 c          976 g          686 t
ORIGIN      
        1 gtcgcggaaa gatcaaaagg attgatcttg atctgcccga tggccgtttc ccggtgcagg
       61 gcttcggaaa tctttttggc caggaccggc ttgagaacgg cgggaagaat cagaaagccg
      121 acaaggccga tcagaacgac gaaaaccagg acagcgatca ggactttttt caaaagacgc
      181 atgaagggct cctttcgaaa gatgccggcg gcaggccgtt ttgatccgca ttccgcgcgg
      241 cttgtcggca atcccgaagc ccgcggctat ttctgaaaaa agacgcgggc gtccaccaga
      301 ctcacgccct ggccttccgg atgcacgatg accggattca ggtcgatttc cgaaatttcc
      361 gggcatgcgg cgagcagagc ggagattctg cataccacat tctgcagcgc ctgacaatcc
      421 gcgggacctc tgcctcttgc gccggccagc agcggatagg ctttcagctc ccggatcatt
      481 ccttcggctt cccccggggt taccggcgcc atccggatgg aggtgtcttt gaaaatttca
      541 atgaaaatgc cgcccagacc aaccacgatg accgggccga aagcgggatc acgcctgccc
      601 ccgacaaaac attcggcgcc ggcgggcgcc atttcctgaa tcagataccc gtcgatggag
      661 ggcgacggct tcaggtttct gattttgctt ttcatgccgg ccagggcttc ggcaagctgt
      721 tttttgcttc gaaggttcac ggcgacgccg ccgatgtccg acttgtgaga ggcgtcccgg
      781 gacagcagct tggcggccag cgggtagcgc agcgtgattc tggacggaat gttttcatct
      841 ttgagcagca ggtttttcac gggctgcagc ccggcggccg cggagaccgc aagcgcctcg
      901 tccgtgagcg gaatccgctt ttcctttccg caacgggcgc ggatgcggtc aaccgctttc
      961 aaatccaggt catacttttc cagatggccc cgcttttggc atgcagtttt cttttcgtaa
     1021 taggatcgcg aggcgtacag ggcctccacc gcttccagcg gagacacgaa aacgggaaat
     1081 ggctgctttt tctgcaggtc aagaatttca tcccgatcgg atatcatcgc caggctgacg
     1141 ggtttttcat agcgggcgac aagctctttc acgcccgcca gaaaggtccg ggacatttcc
     1201 gcctcaaaat cggaggaata cagatgattg aaaagaacgc cgtcaaagtc gtcgagcttg
     1261 agggcttctt ccagaatatc cgtgaagatc gtgtagtcga agatttcgcc gagatccagg
     1321 gggttctggt gggcgatcac gcgggtgttg taaatcgttt tgagcttttc gatgaacgac
     1381 ggcggaaagg gcgtcatttc aaatccgtat tttgcacagg cgtcggccgt caggacggca
     1441 tgaccgccgg accgcgacag cacggccacg cgtttgcctt tcatgagcgg cattccgacc
     1501 atctgaaccg cgctgaccag ctccacttcg tcgtccacgc ggatgacccc ggcctgccgc
     1561 atggctccgt ccaccacggc gtcatcgccg gaaagcgccg ttgtgtgcga ctgggcgatc
     1621 ttcgcgctcg cgggcgaccg gttggatttc tgaaggatga tgggtttgtc gcagtttcgg
     1681 gccagatcga aaaatttgcg tccgcgcttg aagccttcca gatacagcgc aatgcatttc
     1741 gtgcggggat cctgaatcag gtattcgaga aaatccgcct cgtcgagctg cagcttgttg
     1801 ccgatcgaca caaacttgcc ggcggtgatt ccgaatcccg cgaccgttcg cagataggag
     1861 cttccgacac cgccgctctg ggtgatgagc cccagggtgc cgccggaagg gacttccttg
     1921 gcgaaggcga agggcatcat cattttataa tcaaaattga tcacgcccac gcagttgggg
     1981 ccgatgactt tcatgccgta tttgcgcgcg acggccagca cgtcctcttc cagggtatga
     2041 tggccgtgcg agtattcgct gaatcccccc gattcgatca ccacgtgcgt aatgcccttc
     2101 cggccgcatt cctccatgag gccgggaacg gtccttgcgg gcgtcaggaa aatcgccacg
     2161 tccggaacgc acggcagatc cgcgactttg gtgtacacgt gaacgccgtt gatgtcgcct
     2221 tcctgggacc cgacgccgta gatttctccc gggtagccga tttgtttgtt gttgatgagg
     2281 acaatttgcc ccagattcat tttggtgacg gacgcgccga aaacaaccat gctgcggggg
     2341 gtgaagaatt tctccatgat cgaattgctc ctttcgcccg gcaacgctgg tattcaaccg
     2401 cgtttttgag ccagtctgag caaacgacgc cttcaccttg ctgcccgcca tcagccaaag
     2461 cgtgaaacgc caaaatgaca ccaggaaaat gaacttgact tccttttgcc gggcagggct
     2521 cttataaaga acggatgggc ctgtgtcaag gacaaagaga agccggaagc cggggcgaaa
     2581 aatcaggctg cgcggttccg gccgccggtt cccgtccgcc ggcggcaaag attcgtttga
     2641 cttcccgggt aaagccaata taatcatcat ccaagtgatt ttatcagatc cattcttcac
     2701 agcggcgcaa tggggttggc gaaaggcggg tagaggccgg tttccaactt caaagaaagg
     2761 tgatgccatg aagaacaggc ggcaggcaca agaggctccg gaaaaagatg ttcggcagcg
     2821 gcgtccccgc cttcaggcga cccgggagcc tcagcgcccg cggaacctcg cggaaagaga
     2881 gctccgggag agcgaagagc gccttcgcag cgtcgtcagc cggtctccga tccccgcgtt
     2941 tgtgatcggc aaggatcatc gccttcttta ctggaacaag gccctggaac agcttaccaa
     3001 gatcagggcc gaagacgtca tcggcacctc ccagcagtgg cgcgcgtttt acgcgagaaa
     3061 gcgcccctgc atggcggatt tgctggtcag ccgcgcccag gaaaaaatat ccaagtggta
     3121 cacgggcaag tacatccgat ccaaactgct cgacgaggcc tacgaggcga ttgacttttt
     3181 tccggagctc ggtcatcaag gcaagtggct gcgctttacg gcggcgatta tccgcaacga
     3241 agccggcgac ctgatcggcg ccatcgaaac actcgaggac atcacccggc gcaagaaggc
     3301 caaggaggcg ctgctcaagg cgcaccggg
//
LOCUS       DAOPAF010000109         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_23504_length_3259_cov_33.152934, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000109 DAOPAF010000000
VERSION     DAOPAF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_3259_cov_33.152934"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1579)
                     /locus_tag="PLQ77_07815"
     CDS             complement(275..1579)
                     /locus_tag="PLQ77_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPL10622.1"
                     /translation="MFEKEYQEKLTTPQEAVSKIAENALLAHGNLQAEPPALLKAIEN
                     RIRTNDLKKLRVFHLGTSVYASGTILSPELADCVEAYPCFVLTKERGLVYGGLSQFIP
                     NYFHQLRRLIEEYMDEMDVAMTTVSPMDKAGFFSFGTSVDITSIVWKRAKRRIVEVNR
                     HMPRVFGDALVHISDVDQIVENHQPIWESPPAQPDDIDRAIGRIVAEMVPDEATIQLG
                     FGNLPNAVATQLIHHKNLGIHTEAFSPVMVDLVKKGVINGKKKTLHPNKHVYSFALGD
                     HEMMEFMNDNPAFEGYPVSYTNDPDVIARNDNMISINATLQVDLSGQCNSEFLEGAQF
                     SATGGQLDFVRGAFNAKGGKSILTFRSTAKKNTVSRIVPQLDAGSIVTTPRMDTHYLV
                     TEYGAANLKGKCMRDRALAIIGIAHPDFRDQLMREAEKMRLF"
     gene            complement(1615..2271)
                     /locus_tag="PLQ77_07820"
     CDS             complement(1615..2271)
                     /locus_tag="PLQ77_07820"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPL10623.1"
                     /translation="MSSPATDITGIILSGGRNTRMGVNKAFLEIGPSRMMDQTLDIFR
                     RLFSEILIITNDPLAYLEFDDAAVVTDIYKGKGPLGGIYTGLFYAKNPLAFACPCDMP
                     FLNEDFIAYMISRAGKHDIIVPELPQGYQPLHAIYSRSCLPSIKRLLLMDKLKITGFY
                     RDMRVLTIGEEQIRPFNPDGRLFSNLNTPEDVEKLGGKDLKDSRPPSAGGGPKESKTQ
                     "
     gene            complement(2375..>3259)
                     /locus_tag="PLQ77_07825"
     CDS             complement(2375..>3259)
                     /locus_tag="PLQ77_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10624.1"
                     /translation="GDYMKDVETSLRTCAADPESAMKIYDQIWQKMLLVAEQDITRVL
                     PVLKEIAQTISQIPLARKMEDCPKVLIVGEIYVRRDDFAVDELIGQFSRHGIVGKVSN
                     VAEWFYYCDHVRRYELHKRLKLLPWHRRFFARELRDIIDWRIEHIYKKRVEKNVKDAL
                     GATGLVPRTPHDMRTIMKNAEKHFVSRELHSEIAISSGVAATAMMDGYSGIVNISPFA
                     CLIGRVIEGLYTPWARERKYPTISIEIDGNLLPPNVLSKLEIFMLNVKRFRGKGDAST
                     MVERENAQTPAISRKIIR"
BASE COUNT          653 a          868 c          945 g          793 t
ORIGIN      
        1 catccgtttt ctcctttatt cgatgccggg cagagtgttt ctcccgtggc gttggtttga
       61 tgttttccgt ccgttcttcg ggccgacctt ccatttgcct gggcggggaa tccctcccgg
      121 cggacaaagt ttagcataac gcgatcaaaa tgaaaaacag gaattcaagc gcaagggcag
      181 cctgtttccg tgggttccgg tttcttcccc gatccgcatc gtccttgtcg ttgggggaaa
      241 aacccgtttg gaaggctgag gattcaaaaa cccatcagaa gagcctcatt ttttccgctt
      301 ccctcatcag ttgatcgcgg aaatccggat gcgcaatgcc gataatggca agagcgcggt
      361 ccctcatgca ctttcctttc aggttggcgg cgccgtattc cgtgaccaga tagtgggtat
      421 ccatcctcgg cgtcgtgacg atgcttccgg cgtccagctg gggaacgatc ctggagaccg
      481 tgtttttctt cgcggtggag cggaacgtca ggatggattt ccctcccttc gcgttgaacg
      541 ccccgcgcac aaaatcgagc tggccgcccg tcgctgaaaa ctgggctcct tccagaaact
      601 ccgaattgca ctgcccgctt aaatccacct gcagggtggc gttgatggaa atcatgttgt
      661 cgtttctggc aatgacgtcc ggatcgttcg tgtaggatac gggatacccc tcaaaggcgg
      721 ggttgtcgtt catgaactcc atcatttcat gatccccgag ggcaaaggaa tagacatgct
      781 tgttcgggtg cagcgtcttt ttcttcccgt tgatgacccc ttttttcacc agatccacca
      841 tgacgggcga gaaggcttcg gtatgaatgc ccagattttt gtgatggatg agctgcgtcg
      901 cgaccgcgtt gggaagattc ccgaagccga gctggatggt tgcttcgtcc gggaccattt
      961 cagcgacaat tctgccgatg gcccggtcga tgtcatccgg ttgcgcgggc ggagattccc
     1021 agatcggctg gtggttttcg acgatttgat ccacatccga gatgtgcacc agcgcgtctc
     1081 cgaaaacgcg gggcatatgc cggttgacct caacgatgcg gcgtttggcc cgcttccaga
     1141 caatggacgt aatatccacc gatgtgccga aagaaaaaaa gcccgctttg tccatggggc
     1201 tgaccgtcgt catggcgaca tccatttcat ccatgtactc ttcgatcagg cggcgaagct
     1261 gatgaaaata gttcgggatg aattgtgaaa ggcccccgta aaccaaaccc ctttccttgg
     1321 tcaggacaaa gcaaggatag gcctcgacgc agtcggccag ttccggtgaa aggatggtcc
     1381 cggaggcata tacgctggtg ccgagatgaa atacccgaag ctttttcagg tcattggtgc
     1441 ggattcggtt ttcgatggcc ttcaacagcg ccgggggctc cgcctgcaga ttgccgtgcg
     1501 ccagcaaagc gttttcggca attttcgata cggcttcctg aggagtggtg agtttttcct
     1561 gatactcttt ttcaaacata ttcgtcccct ttcaggcgca tgacgccctg gtcgttattg
     1621 cgtcttggat tccttcggac cgccgcccgc ggacggcggc cgggagtctt tcaaatcttt
     1681 tccgccgagc ttttccacat cttccggcgt gttgaggttg gagaaaagac gtccgtccgg
     1741 attgaacggc cggatctgtt cttcgccgat ggtcagcacg cgcatgtcgc ggtaaaagcc
     1801 ggtgatcttg agtttgtcca tgagcagcag gcgcttgatg gagggcaggc agcttcgtga
     1861 ataaatcgcg tgcaggggct gatagccttg gggaagctcg ggtacaatga tgtcatgctt
     1921 tcccgcccgg ctgatcatgt acgcgataaa atcttcattt aagaaaggca tgtcgcacgg
     1981 acaggcgaag gcaagcggat tcttcgcgta gaaaagaccg gtgtagattc cgcccagcgg
     2041 ccccttgccc ttgtagatgt cggtcacgac ggctgcgtcg tcaaactcca gataggcgag
     2101 ggggtcgttc gtgatgatga ggatttcgga aaagagccgg cgaaagatat cgagcgtctg
     2161 atccatcatg cgggagggac cgatttccag gaaggccttg ttgacgccca tccgggtgtt
     2221 cctgccgccg gaaaggatga tgccggtaat gtcggttgcg gggctgctca atggatccgt
     2281 ttccttcttc gtttttgtaa atggcatacc ataaaatgca caaagagatt aagcgaaaaa
     2341 aggcaaggca ggaggcaaag ccggccgtaa ggttttaccg aatgattttc cggctgatgg
     2401 ccggcgtctg tgcgttttcg cgttccacca tggtggaggc atcccccttg ccccggaacc
     2461 ttttcacgtt gagcatgaaa atttccagct tggacaggac gttgggcggc agcaggttgc
     2521 cgtcgatctc gatcgaaatg gtcggatact tcctttccct cgcccagggc gtgtacagcc
     2581 cctcgatgac gcggccgatc aggcaggcga acggagagat gttgacgatg ccggagtagc
     2641 cgtccatcat ggcggtggcg gcgacgccgc tggaaatggc gatttccgaa tgcaactccc
     2701 ggctgacgaa atgtttttcc gcgtttttca tgattgtccg catgtcatgc ggcgtgcgcg
     2761 gcaccaggcc ggtcgccccc agcgcgtctt tgacattttt ttccacgcgt tttttgtaga
     2821 tatgctcgat gcgccagtcg atgatgtccc gcaactcccg ggcaaagaag cgccggtgcc
     2881 agggcagcag tttgaggcgc ttgtgaagtt cgtagcggcg gacatggtcg cagtagtaga
     2941 accattcggc gacgttcgac accttgccga caatgccgtg gcggctgaac tggccgatca
     3001 gttcgtccac ggcgaagtcg tcgcgacgga cgtagatttc gccgacaatg agcactttgg
     3061 ggcagtcctc catttttctg gccagcggta tttgcgagat ggtttgcgcg atttccttca
     3121 ggaccggcaa aacccgcgta atatcctgtt cggccaccaa gagcatcttc tgccagattt
     3181 gatcgtaaat cttcatggct gactcgggat cggcggcgca cgttcgaagc gaggtttcca
     3241 catccttcat atagtcgcc
//
LOCUS       DAOPAF010000110         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_24246_length_3175_cov_81.793590, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000110 DAOPAF010000000
VERSION     DAOPAF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24246_length_3175_cov_81.793590"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLQ77_07830"
     CDS             complement(<1..794)
                     /locus_tag="PLQ77_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL10625.1"
                     /translation="MKGNRLPWPIDLAGFYWRRKIRGQSVPLLASFKVTHRCNLSCRA
                     CPFHLHADQSHMPRDTAVHALEALRRLGTRIVVFEGGEPLLWRDGGYRLHDLVAYARR
                     RFLRVAVTTNGTLPLDVPADTLWVSLDGWKETHDELRSGSFDAVYSRLKETGHPRVFV
                     HCTINRINWRDLERMAAQVRSLPAVRGMTFQFFYPYGLGEDDLALAPDERRAAVETLL
                     DLKKRGFPILNSAGSLAAMTQKRRSCRDDILINVDPDGTITRGCYV"
     gene            complement(816..2039)
                     /locus_tag="PLQ77_07835"
     CDS             complement(816..2039)
                     /locus_tag="PLQ77_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPL10626.1"
                     /translation="MTRILDPTDKYYIDNPGRVKAIQAEMARRGIDVYLGSRIRTMSF
                     VLDTFCPWRSYVIIPPEGQPAFFTFVVDALRIADETWIDSDHVRAYGPMGGMDQVSAI
                     TDFIKEELGIRKGRLGFEDGISTYTAEGNLSHWEYTHFKDALPDFEWINSHDIVDGLS
                     LIKDKGTIERFRTASLIVDEGHKAARAALINGGFKGMTETVIAGIAALAMRKAGSVSE
                     WNFAGLNEISSGFRTGLGACTPPTTKEIGAGEPLMLDFHAMFKLALGDHSHNYLIGPA
                     TKRQRWHADNFVAIIQKVIDNYRAGTTPGTLAAIMMDFAESRGCGDFIQPACEHGIGL
                     FGDEWRIGAVVNPDLPYWTDQDHVYQENEMVVCAMQYAAPEENIGFRYENDIVITKDG
                     CEVLSKYPLGIEEIS"
     gene            complement(2055..2453)
                     /locus_tag="PLQ77_07840"
     CDS             complement(2055..2453)
                     /locus_tag="PLQ77_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPL10627.1"
                     /translation="MSLLAYLSPSWRDEALQRLQTELTPEKMNNVTTSMSNIYKNCPG
                     GDEQFLFVECRDGKVTRVETGSGEPPQAEFRIIGDYETFAKISRAELGSQKALMTGKL
                     KLKGNLARALKLAAVADRLNKVLAKIPTQY"
     gene            complement(2568..>3175)
                     /locus_tag="PLQ77_07845"
     CDS             complement(2568..>3175)
                     /locus_tag="PLQ77_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10628.1"
                     /translation="YNKVDAQSKQAQAGATTKPRPKKPTGLKGASTRVKEAPLTWQAN
                     PEKDIQVYLIQRAEGDRDNFSSIGEAAATLYTDANLKDGTTYRYRIQAKDADNLLSDF
                     SDVIVVTTKPRPQPPENLQGKYESGKAVLSWTPNKEADISHYIIYEKKFIGTEKIAES
                     NTASYADGSLSPGKSRNYVVTAVDRSGLESDVSAELKVIAK"
BASE COUNT          558 a          926 c          984 g          707 t
ORIGIN      
        1 ttgacgtagc agccccgggt gatggtgccg tccggatcga cgttgatcag gatgtcatcc
       61 cggcagctcc ggcgtttttg cgtcatggcg gcaagacttc ccgcggagtt taagatgggg
      121 aagcctcgtt ttttcagatc cagcagcgtc tcgacggcgg cccggcgctc atccggcgcc
      181 agggcaaggt cgtcttcgcc caggccgtac ggataaaaaa actgaaaggt cattcccctc
      241 accgcgggga ggcttcggac ctgtgcggcc atccgttcca ggtctctcca gttgatgcgg
      301 ttgatcgtgc agtggacaaa aacacgggga tgcccggttt ccttcaggcg ggaataaacg
      361 gcgtcaaagg atccgctgcg cagctcgtca tgcgtttcct tccatccgtc gaggcttacc
      421 cagagcgtgt cggcgggaac gtccagcggc agcgtgccgt tggtggtcac cgccacgcgc
      481 aaaaatcgcc ggcgggcata ggctaccaga tcgtgcagac ggtatccgcc gtcgcgccag
      541 agcagcggtt ccccgccttc aaaaacgacg atgcgcgttc cgaggcggcg cagggcctcc
      601 agggcgtgga cggccgtgtc ccgcggcatg tggctttgat ccgcatgcag atgaaagggg
      661 caggcgcggc aggacagatt gcagcggtgc gtcaccttga agctcgccag cagcgggacg
      721 gactgcccgc ggattttccg ccgccagtaa aatcccgcca gatcgatcgg ccagggaaga
      781 cggttgccct tcatgatgtc cgcgtctttc ccgcgctacg agatctcctc gatccccagc
      841 ggatatttgg acagaacctc gcagccgtct ttcgtgatga cgatgtcgtt ttcgtagcgg
      901 aagccgatgt tttcctccgg cgcggcgtac tgcatcgcgc agacgaccat ttcgttctcc
      961 tgatagacgt ggtcctgatc cgtccagtag ggaagatcgg gattgacgac ggcgccgatg
     1021 cgccactcgt cgccgaaaag cccgatgccg tgctcgcagg cgggctggat gaaatcgccg
     1081 cagccgcgcg actcggcaaa gtccatcatg atggcggcca gcgtgccggg cgtcgttccg
     1141 gcccggtagt tgtcgatcac tttttgaatg atggcgacaa aattgtccgc atgccaccgc
     1201 tgtcgtttgg tcgccggccc gatgaggtag ttgtgcgaat gatcgcccag cgccagtttg
     1261 aacatggcgt gaaaatccag catcaggggc tcgcccgcgc cgatttcctt ggtcgtgggc
     1321 ggcgtgcagg cgcccagtcc cgtgcggaaa ccgctggaaa tttcattgag ccccgcaaag
     1381 ttccattcgg aaacgctgcc cgccttgcgc atcgccagcg cggcgatgcc ggcgatcacg
     1441 gtttccgtca tgcccttgaa cccgccgttt ataagcgcgg cgcgggccgc cttgtggcct
     1501 tcatcgacga tcagcgacgc ggtgcggaag cgctcgatcg tgcccttgtc cttaatgagc
     1561 gacaggccat ccacgatgtc gtgcgagttg atccattcaa aatcgggaag cgcgtcttta
     1621 aaatgcgtgt actcccagtg cgagagattt ccctcggccg tgtaggtgga aatgccgtct
     1681 tcaaagccca ggcgcccctt ccggatgccg agctcttctt tgatgaagtc cgtgattgcc
     1741 gacacctggt ccatgccgcc catcgggccg taggcgcgga cgtggtcgga atcgatccag
     1801 gtttcgtcgg caatgcgcag ggcgtcgacg acaaacgtaa agaaggcggg ctggccttcg
     1861 ggcggaatga tcacatagct gcgccagggg caaaacgtat ccagcacaaa gctcatggtg
     1921 cggatgcgcg agccgagata aacgtcgatg ccgcgccgcg ccatttcggc ctggatcgct
     1981 ttgacccggc cggggttgtc gatgtaatat ttatcggtag ggtccagtat tctggtcatg
     2041 atgcgatctc ccttctaata ctgtgttgga atcttcgcga gcaccttgtt gaggcggtcg
     2101 gcgacggcgg ccagcttgag cgcgcgggcc aggttgccct tgagcttgag cttgccggtc
     2161 atgagcgcct tttgcgagcc cagctccgcg cgggaaattt tcgcaaaagt ctcgtaatcc
     2221 ccgatgatcc ggaactccgc ctgcggcggt tcgcccgagc ccgtttctac gcgggtgact
     2281 ttcccgtccc tgcattccac aaaaaggaac tgctcatcgc cgccggggca gtttttataa
     2341 atgttggaca tggaggtggt gacgttgttc attttttccg gggtgagctc cgtttgaagt
     2401 ctttgaagcg cctcgtcgcg ccaggacggg cttaaatagg ccagtaaaga catgtggatt
     2461 cctcctttga tgttttccgg cggccggggc aaagacagcc ccggccgaat gaatgttgcg
     2521 gtcagaggga tatttgattg cagccagatg cggccggagg ggccccgtca tttggcgatc
     2581 accttcaact ccgcgctgac atcgctttcc agaccgctcc ggtcaacggc ggtgacgacg
     2641 taattcctgc ttttccccgg agacaacgat ccgtccgcat aagaagcggt gttcgattcc
     2701 gcgatttttt ccgtcccgat aaactttttt tcgtagatga tgtaatggga aatgtccgct
     2761 tccttgttgg gcgtccagga caggaccgcc tttccggatt cgtatttccc ctgcaggttc
     2821 tcgggcggct gcggcctcgg tttcgtggtc acgacaatca catcggagaa gtccgacagc
     2881 aggttgtccg cgtctttggc ctgaatccgg tagcgatagg tcgtgccgtc ctttaaattg
     2941 gcgtccgtat agagggttgc ggcggcctcg ccgatgctgg agaagttgtc cctgtctccc
     3001 tcggcccgtt ggatcaggta aacctgaatg tctttttcgg gattggcctg ccaggtcaga
     3061 ggcgcttcct tgacgcgggt agaggcgcct ttcagcccgg tcggtttttt cggccggggt
     3121 ttggtcgtgg cgccggcctg agcctgcttg ctttgcgcgt ccaccttgtt gtagg
//
LOCUS       DAOPAF010000111         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_24312_length_3168_cov_48.664632, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000111 DAOPAF010000000
VERSION     DAOPAF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24312_length_3168_cov_48.664632"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLQ77_07850"
     CDS             <1..289
                     /locus_tag="PLQ77_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10629.1"
                     /translation="AYLAKDAKVQLTGDFSFYGSSARPEKKGFNIFGILLFLIAAAVL
                     LGTRTGREMLPWILLMLLSGSGRGGGSGGFGGGFGGFGGGMSGGGGAGRSF"
     gene            307..1035
                     /locus_tag="PLQ77_07855"
     CDS             307..1035
                     /locus_tag="PLQ77_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10630.1"
                     /translation="MAKIPDQPQDIFVPLTEDYLEAFGRELVSLMLYGSAAGGSYVKG
                     KSDINLLVVLTPEGMNRLEDAFAPVKKWRKRNVAVPLFLTKEFISSSLDAYPIEFLNM
                     QRHCVLIYGENVLAPLAFKPEDLRLQIEREIKSKIILLREGYLESEGSARRVRELIGR
                     SLVALVSIFNAMLYLKQAGLPRHKKETIEEAGRVFGVDAAVFARCFDIKAKEDHLSGK
                     EVIGVFKQYLREVEKVCHKIDGLK"
     gene            1060..1632
                     /locus_tag="PLQ77_07860"
     CDS             1060..1632
                     /locus_tag="PLQ77_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPL10631.1"
                     /translation="MSAGKKIIIVVAVIAVLAISLYSLFAGNYNRFVRMDVAIKSAWA
                     QVENQLQRRYDLIPNLVETVKGYAKQEKDVLVEVTNARSKVGGAGTVPDKIAANNELT
                     GALSRLMVVVERYPDLKSNQNFLQLQDELAGTENRIAVERMRYNDAVKIYNQAVRTFP
                     ANIVAGMFGFKEAAFFEAPKEAQAPPQVKF"
     assembly_gap    1785..1884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1885..2723)
                     /locus_tag="PLQ77_07865"
     CDS             complement(<1885..2723)
                     /locus_tag="PLQ77_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10632.1"
                     /translation="MPASGQPAAPKESATVILVCSHPADAWNVFLARRHSRSSFMAEA
                     YVFPGGQIADADRDEDLRNFISMPPGFDPRAHLKDDSLSQETAQSLFVCAIRETFEET
                     GVLIARLRDGSPLQLTSKEQNARLAAYRGELNAGRTTLSEMARRENLVFPLDLLVPYA
                     HWITPEFLPKRFSTRFFLAALPEDQSAATDQGELTDSLWAKPEEVLRRYRGREIVLMP
                     PTLKTLEELARYACIDDLFAGVRSRAIYPILPEPGENMIKLPHDPEYGIERYKQPSRP
                     EEPS"
     gene            complement(2741..>3168)
                     /locus_tag="PLQ77_07870"
     CDS             complement(2741..>3168)
                     /locus_tag="PLQ77_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL10633.1"
                     /translation="RGVWVLTQKVARIMKEQGGGNIINISSVMAFRGSIEEAHPAVPY
                     NSTKAAINLLTMNLAVKLAPHKIRVNAIAPGFFHTDMMAYIDKPEFKPVRDAIINEIP
                     LRRVGEIDDMKGVAVFLASDASQFITGHVMVVDGGQIAK"
BASE COUNT          635 a          815 c          934 g          684 t
ORIGIN      
        1 cgcctatctc gccaaagacg cgaaggttca gttgaccggc gatttctcct tttacgggag
       61 ctccgcccgt ccggaaaaga agggcttcaa tatttttgga attctgctct ttctcattgc
      121 cgcggccgtg cttctgggaa cgagaaccgg aagggagatg ctgccctgga ttctcctgat
      181 gctgctttcc ggcagcggcc ggggcggcgg ctccggcggc ttcggcggcg ggtttggcgg
      241 cttcggcgga ggcatgagcg gcggcggagg agcgggccgc agtttttaat ataaaggatg
      301 gagaacatgg caaaaatacc ggatcaaccg caggacattt tcgttcccct gaccgaggac
      361 tatctcgaag ccttcggcag ggaattggtt tcgttgatgc tctacggcag cgcggcgggc
      421 ggctcctatg tgaagggaaa atcggatatc aatcttctgg tcgttttgac gccggaaggc
      481 atgaaccgcc tggaagacgc ctttgccccg gtgaaaaaat ggagaaagcg caatgtagcc
      541 gtgccgctgt tcctgacgaa ggaattcatt tcttcctcgc tcgacgctta tccgattgaa
      601 tttctcaaca tgcagcgcca ttgcgttttg atttacgggg aaaatgttct ggcgccgctg
      661 gcgttcaaac cggaggatct ccgcctgcag atcgaacggg aaatcaaatc caagatcatt
      721 ttgcttcggg aggggtatct ggaatccgaa ggatcggcgc gccgcgtcag ggaactgatc
      781 ggccggtcgc tggtcgccct ggtttccatt tttaacgcca tgctgtattt aaagcaggca
      841 ggccttcccc ggcacaagaa ggaaacgatt gaggaagcgg gcagggtttt cggcgtcgac
      901 gcggctgtct ttgcgcgctg tttcgacatc aaggcaaaag aggaccatct ttccggcaaa
      961 gaagtgatcg gggtgttcaa acagtatttg cgggaagtgg aaaaagtctg tcataagatc
     1021 gatggattaa aataattcaa gcgatggagg atgaaaaaaa tgtcggcagg aaagaaaatc
     1081 atcattgtgg tggcggtcat cgccgttttg gcgatttctc tgtattccct ttttgcgggc
     1141 aactacaacc gatttgtaag aatggacgtc gccatcaagt cggcttgggc acaggtggaa
     1201 aatcagctgc agcgccgcta cgacctgatt cccaatctgg tggaaacggt caaaggctac
     1261 gccaagcagg agaaagacgt gctggtggaa gtgaccaacg cgcgctcgaa agtcggcgga
     1321 gccggaacgg ttcccgacaa gatcgcggcc aataacgaat tgaccggcgc tctcagccgt
     1381 ctgatggtgg tggtggagcg ctacccggat ttgaagtcga atcagaattt tttgcagctg
     1441 caggatgagc tggccggaac ggaaaaccgc attgccgtgg aaagaatgag atacaacgat
     1501 gcggtgaaaa tctacaatca ggccgtccgg acgtttccgg ccaatatcgt cgcgggcatg
     1561 ttcgggttta aggaagccgc tttctttgaa gcgcccaaag aagcccaggc tccgcctcag
     1621 gtcaaattct gatcccttta ttcatgaggg gaacggcgtc aatccgttcc cctcatgctt
     1681 ttcgccgttt tgattcagct ttgattcagc gccggtgaag ccgcgtgctt caccggcttt
     1741 gtccggttcc atttcagagt ttgccgcccg tccgccagat gccgnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnngacggc tcttcgggcc gggacggttg tttgtatctt
     1921 tcgatgccgt attccggatc gtgaggcagc ttgatcatgt tttccccggg ctcgggcagg
     1981 atggggtaaa tggcgcggct tcgaaccccg gcaaagaggt cgtcaatgca ggcgtatcgg
     2041 gccagctcct ccagcgtctt cagggtcggc ggcatcagga cgatttccct gccgcggtac
     2101 ctgcgtaaaa cttcttccgg ctttgcccag aggctgtccg tcagttcgcc ctgatcggtg
     2161 gcggctgact ggtcttccgg aagagcggcc agaaaaaaac gggtactgaa tcgtttcggc
     2221 agaaattccg gcgtaatcca gtgggcgtag ggaaccagca agtccagggg aaagaccaga
     2281 ttctcccgcc gggccatctc gctcagagtg gtccggccgg cgttcagttc ccctcgatag
     2341 gcggcaaggc gggcgttttg ctctttcgaa gtcagctgga gggggcttcc gtcccgcagc
     2401 cgcgcaatga ggactcccgt ttcttcaaag gtttcccgaa tggcgcagac aaacaggctc
     2461 tgcgccgttt cctgggaaag ggaatcgtcc ttcaggtgcg cccgcggatc gaagcccggc
     2521 ggcatggaaa tgaaattccg gagatcttcg tcccggtctg cgtctgcaat ctgaccgcct
     2581 ggaaaaacat atgcctccgc cataaaggac gaacggctgt ggcggcgggc cagaaaaaca
     2641 ttccaggcgt ccgccggatg gctgcacacc agaataaccg tcgccgactc tttcggcgcg
     2701 gcaggttgtc cggatgcggg cacgccggct ccttttcttt ctatttcgcg atctgtccgc
     2761 cgtccacgac catgacatgt ccggtgatga actgcgatgc gtcggaggcc agaaacaccg
     2821 ccacgccttt catgtcgtcg atttcaccga cgcggcgcag gggaatctcg ttgatgatgg
     2881 cgtcgcggac cggtttgaat tccggcttgt cgatataggc catcatgtcc gtgtggaaaa
     2941 agcccggcgc aatggcgttg acccggattt tgtggggcgc cagcttcact gcgaggttca
     3001 tggtcagcag gttgatcgcc gccttggtgg aattgtaggg aacggccgga tgggcttcct
     3061 cgatggagcc gcggaacgcc atgacggagg agatgttgat gatgttgccg cctccctgct
     3121 ccttcatgat gcgggccact ttctgcgtca gcacccagac gccccgca
//
LOCUS       DAOPAF010000112         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_24615_length_3136_cov_41.435248, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000112 DAOPAF010000000
VERSION     DAOPAF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_3136_cov_41.435248"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PLQ77_07875"
     CDS             <1..1040
                     /locus_tag="PLQ77_07875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPL10634.1"
                     /translation="GEEVVSNYEIKYLTKTGETRIGDFGATFIHYEGRPAMIASISDT
                     TERKKKKEELRASEERYRTIIENIEDGYFEVNLKGDLIFASDPCMKITGTDRKTFIGM
                     NFREFSPRENWPAIYQAFYRVFETGEPLKGLSWEVVRIDGVHQYIEISTSLIRDHEGR
                     PVGFRGIVRDVTERKQREDMIQWMAYHDILTGLPNRALFYDRAAMVLAQAKRKQWRFG
                     LMMLDLDRFKEVNDTYGHDAGDKLLIGVARCLEEALREQDTVARIGGDEFVVILPEVS
                     SREDVRMIRERIAKGFQRPIPIGETEMVAACSIGVALYPDDAGDIESLMHFADEAMYR
                     AKIEGGGLCSN"
     gene            1078..1983
                     /locus_tag="PLQ77_07880"
     CDS             1078..1983
                     /locus_tag="PLQ77_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL10635.1"
                     /translation="MKQQAKGKIHERITAIPNAWYPVYLVRGEKKNLMIDAGVNLLGP
                     RYLTLIQDILGGATKLHYLFLTHSHYDHVGSAGYLKRRIPGLKIGAHERVGGLLHKPS
                     VLDMMNRLSANHVELLKYNAAGEDLTLKPFDIDILLKQGDELDLGGLTCRVYETPGHT
                     KDSLTFYFPEIGVLFPGESAGVLAGETAGEMQVEFLASYRDYIDSLKLMISLKPGLIC
                     LGHGWVLGGRDAEDFLRRSLAETFRYRELIEDYLNAAGGDVETAIQDMAHAQYDVKGG
                     IFQERISYVTNLSAQVRHIAGLTAP"
     gene            2038..2808
                     /locus_tag="PLQ77_07885"
     CDS             2038..2808
                     /locus_tag="PLQ77_07885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPL10636.1"
                     /translation="MSKFIDLSVAIESGLPSDPPMMIPKIMYVDHALGAESMKAFYPG
                     LTASDLPEGLGWALEILEVSTHAGTHMDAPWHYHPTQDRGKPALTIDQFPLEWGVGNG
                     VKLDFSGFPDGYNITVEDVQTKLKEINYSLRSGDVFLAQSGAAPFWGTPEYLVKGCGF
                     GRAATLWILDQGVHVVGTDGWSWDRPLPFQAEEFAKTRDASLIWEGHFAGIDKGYFQI
                     EKLANLEKIPSTGFRFYCFPVKIKGASAGWIRAVAEIL"
BASE COUNT          725 a          792 c          936 g          683 t
ORIGIN      
        1 gcggggaaga agttgtatca aattatgaaa tcaaatatct gacaaaaacc ggcgagacaa
       61 gaatcggcga tttcggggcg acctttatcc attatgaagg ccggccggcc atgatcgctt
      121 cgatttccga caccacggag cggaaaaaga agaaagaaga attgcgggcc agcgaggagc
      181 gttaccgcac aattatcgag aatatcgagg atggctattt cgaggtgaat ctcaaaggag
      241 atcttatttt tgccagcgat ccctgcatga aaattaccgg caccgaccgg aaaaccttca
      301 tcggcatgaa ttttcgcgag ttctcgccga gggaaaactg gcccgcgatt taccaggcgt
      361 tctacagggt ttttgaaacc ggagagcctt tgaaaggcct gtcctgggaa gtggttcgta
      421 tcgatggggt tcatcagtat atcgaaatat ccacatcgct gattcgggat cacgaggggc
      481 ggccggtcgg gttccggggc atcgtacggg acgtgacgga gcgcaagcag cgcgaagaca
      541 tgatccagtg gatggcttac catgacattc tgacggggct tcccaaccgg gctctttttt
      601 acgaccgcgc cgccatggtg ctggcccagg ccaaacggaa gcaatggcgt ttcggactca
      661 tgatgctcga tctcgatcgc ttcaaggaag tcaacgacac ctacggacat gatgccggag
      721 acaagctcct gatcggggtg gcccgctgcc tggaagaggc gctgcgggag caggatacgg
      781 tggcccgcat cggcggggac gagttcgtcg tcattcttcc ggaagtttcc agccgcgagg
      841 acgtaagaat gatccgagag agaatcgcca aaggttttca aaggccgatt ccgatcggag
      901 agacggaaat ggtcgcggcc tgcagcatcg gcgtggccct ttatcctgat gatgccgggg
      961 atattgaaag tctgatgcac tttgcggacg aggcgatgta cagggccaaa atcgaaggag
     1021 gcggtctttg cagcaattga tgccagagag gcttccgtcc gcccggtccg gttctttatg
     1081 aaacagcagg ccaaaggcaa aatccacgag cggatcaccg ccattccgaa tgcctggtat
     1141 cccgtttatc tggttcgggg agaaaagaaa aatctgatga tcgacgcggg cgttaatctt
     1201 ctgggtcccc gctacctcac gctcattcag gatattctcg gcggcgcaac aaagctccac
     1261 tatctttttc taacccattc gcattatgat catgtcggct ctgcgggcta tttaaaacgg
     1321 cgcattccgg gcttaaagat cggggctcat gagcgggtcg gcggcctgct gcacaagccc
     1381 agcgttctgg acatgatgaa ccgcctcagc gccaaccatg tcgagcttct gaagtacaat
     1441 gccgccggtg aagatctgac gctgaagccg tttgacatcg atattctgct gaaacagggg
     1501 gacgaactgg atctgggcgg tctcacctgc cgcgtttatg agacgcccgg ccacaccaaa
     1561 gactcgctga cgttctactt tccggagatc ggggtcttgt ttcccggcga atccgccggc
     1621 gtccttgcgg gtgaaacggc cggcgaaatg caggttgaat ttcttgcctc ctaccgggac
     1681 tatatcgatt cgctgaaact catgatttct cttaagcccg gcttgatctg cctggggcat
     1741 ggatgggtgc tgggcggcag ggacgcggaa gattttctcc ggcgctctct tgcggaaacc
     1801 ttccggtacc gggagcttat cgaagactat ctgaatgccg cgggcgggga cgtggagacg
     1861 gccattcagg atatggccca tgcccaatac gacgtcaagg gcggcatctt tcaggaacgg
     1921 atttcctacg tgaccaatct gagcgcgcag gtcaggcata ttgccggatt gactgcaccc
     1981 tgaatccttc cggcgatcac catcaacgaa gcaacgactg atatggggag gatgatcatg
     2041 agcaagttca ttgacctttc agtggcgatc gaaagcggcc tgcccagcga tccgccgatg
     2101 atgattccca aaatcatgta tgtggatcat gcgctggggg ctgaaagcat gaaggcgttc
     2161 tatcccggcc tgaccgcttc cgacctgccg gaaggattgg gttgggcact cgaaattctg
     2221 gaggtctcga cgcacgcggg tacacatatg gacgcaccct ggcattatca tcccacacag
     2281 gaccggggga aaccggcttt gaccatcgat cagtttcccc tggaatgggg agtgggaaac
     2341 ggcgtgaagc ttgacttttc gggctttccc gacggataca atatcacggt tgaagatgtt
     2401 caaaccaaac tcaaggaaat aaattactcg cttcggtcgg gggatgtttt tctggcgcaa
     2461 agcggcgccg cgcctttctg gggcacaccc gaatatcttg taaagggatg cggatttggc
     2521 cgggcggcga ctctctggat tctggaccag ggcgttcacg tggtcgggac ggacggctgg
     2581 agctgggaca ggcctctgcc ctttcaggcc gaagagtttg cgaagaccag ggacgcttcg
     2641 ctgatctggg aagggcattt tgcggggatc gacaagggct atttccagat tgaaaagctg
     2701 gccaatctgg aaaaaattcc gtcaaccggc ttcaggtttt actgcttccc ggtgaaaata
     2761 aagggcgcga gcgccggatg gatcagggcg gtcgcggaga ttttataaaa cggacccgat
     2821 gggcctggtt ttcccgatgg aggcgtgaaa tgaccggcgc ctatgttccg taaacactat
     2881 tagtgagtta cggcctgttg cccaagcgaa aagggtattc gggcaacaag ccgccagcac
     2941 tgggcgcgtg gttttgataa aaagaccccc tcagttgtag aaacctgagg gggcgtacta
     3001 ttaacacaga tcctgaaggt tgccgaagcg gcggttactt catcgcttgc tgcttgcttt
     3061 gatcaggtga caatgttttt caccgcgtca atgaccggat tggtgaagag cgccatcaga
     3121 agggtgtaaa gagcga
//
LOCUS       DAOPAF010000113         2984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_26171_length_2984_cov_42.304199, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000113 DAOPAF010000000
VERSION     DAOPAF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26171_length_2984_cov_42.304199"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLQ77_07890"
     CDS             complement(<1..449)
                     /locus_tag="PLQ77_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPL10637.1"
                     /translation="MQEKNTDQSSMNADHVRRLTQIGVALSAEKNIDRLLEKILTEAR
                     TITSADGGTLYLMSENERELQFAFIQNESMGVTMGGTGEKITWPPVPMTGADGLPNHA
                     NVSAHVAITREAVNIEDVYDASGFNFDGPRAFDQKTGYRTKSMLVVP"
     gene            complement(569..943)
                     /locus_tag="PLQ77_07895"
     CDS             complement(569..943)
                     /locus_tag="PLQ77_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPL10638.1"
                     /translation="MNIVDSSGWLAYFADEINAKHFMAPLSSPTRLIVPAITLYEVFK
                     VILRESSENEALQAVVAMQKGQVINLNTSLALAAARLSLEHRFPMADSIILATALEFR
                     ATIWTQDADFKDLNKVKYFPKK"
     gene            complement(940..1146)
                     /locus_tag="PLQ77_07900"
     CDS             complement(940..1146)
                     /locus_tag="PLQ77_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPL10639.1"
                     /translation="MQSVTVSPKYQVVIPKEVRDSLKLQPGQKMQVVEYAGRIELIPE
                     RDIKELRGFLKGINTEFKREEDRL"
     gene            1327..1944
                     /locus_tag="PLQ77_07905"
     CDS             1327..1944
                     /locus_tag="PLQ77_07905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL10640.1"
                     /translation="MLKIDPIKISKIKGFLDEAEAGRLYDLALEASRSAPCLEIGSYC
                     GKSSVYLGCACRENGAIFFSVDHHTGSEEQQPGQEYFDPELLDKETGKIDTFRLFRKT
                     IEDFDLQDTVVPIIGRSDIIGRAWKTPLGLVFVDGSHAYESVLKDYQVWADHVRSGGY
                     LVFHDIFPNPADGGQAPYLVYQQAVASGLFDAAPLFKSLGVLKKK"
     gene            complement(2005..>2984)
                     /locus_tag="PLQ77_07910"
     CDS             complement(2005..>2984)
                     /locus_tag="PLQ77_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL10641.1"
                     /translation="FCLGRKMVGAKVRRRNPALVVDEIEQIIGMGFDRMNIADDLFAS
                     DTNRVREICRGIRERGLKFTWSAFARVDTVTQEMFDEMASVGCDSVSFGVESGCPEML
                     KRVRKGITLAQAAEAVRMCKQSGMLAHASFMVGLPGETKETLQRTDEFARSLDIMYGY
                     HYLAPFPGTTLCEKVERYDLQILTRDWSKYDANDAIVKTSELVPQDIRDFVARYEAEV
                     NGDWQKVVDNYKTGKNTPYDDLRVEGQSRTKITYAILKEDLVERLGVIDSSLAGGGSK
                     DEAENLLIERMKESISGDPAVVESTVRDFIAKGYLRADFSDRGCIWRWS"
BASE COUNT          644 a          823 c          754 g          763 t
ORIGIN      
        1 atcggaacga ccagcatgga tttcgtgcgg tagcccgtct tctggtcgaa cgccctgggc
       61 ccgtcaaaat tgaacccgga cgcgtcataa acatcctcga tgttcaccgc ctcccgggtg
      121 atcgccacgt gcgcggatac gttcgcgtga ttgggcagac cgtcggcgcc ggtcatgggc
      181 acgggcggcc aggtgatttt ctcgcccgtc ccccccatgg ttacgcccat ggattcattc
      241 tggatgaagg cgaactgaag ttcacgctcg ttttccgaca tgaggtagag cgttccgccg
      301 tccgcggacg tgatggtgcg ggcttccgtg agaatttttt ccagcagacg gtcgatattc
      361 ttttcagcgg aaagcgccac gccgatttgc gtcagccgtc gaacgtggtc agcgttcatc
      421 gaactctggt ccgtgttttt ttcctgcatt aaagcaatgc ccgccctgat cgatgatttt
      481 tttgtactat ctctgaaaca ggctttgctg tcaaccgtga agttgcaggt gcgtaagcac
      541 aggtgttaaa agcaaatgag ttgtgttttt attttttcgg gaaatatttg accttgttta
      601 aatctttaaa gtccgcatct tgagtccaga tagtagccct gaattccagg gctgttgcaa
      661 gaataatact gtctgccatg ggaaaccgat gttccagact cagtcttgcc gcggccagcg
      721 ccagtgatgt atttaaattg atgacttgtc ctttttgcat ggcaacaacc gcctgcaatg
      781 cctcattctc actggattcc ctcaggatga ctttgaatac ttcgtatagg gttattgcgg
      841 gaacgataag cctggttgga ctgctcaatg gcgccataaa atgctttgca ttgatttcat
      901 cggcaaaata agcaagccag cctgaagaat ctacaatatt cataatcgat cttcctcgcg
      961 cttgaattcc gtattgatgc ctttgagaaa tccacggagc tctttgatat cacgttcggg
     1021 aatcagttca atccttccgg cgtattctac gacttgcatt ttttgacccg gctgaagctt
     1081 taaggagtct ctgacttctt tggggatgac cacctgatat ttcggggata ccgtgacgga
     1141 ttgcatgcca atacctccat cgatatattg ttggtattac gatatcacaa tcgataggtt
     1201 ttggtcaatg attcgatgat tcaaattctg atccaaccaa accccttcgc ccttttcgtc
     1261 atctttcaag ttgcaattta ttcgggggcg gtttatgaaa acaaaacttc cctcaaagga
     1321 ttgccgatgc tgaagattga cccgataaaa atatcaaaaa tcaaaggatt tctggatgag
     1381 gcggaagccg gtcgcctcta tgatctggcc ctcgaagcct cccggtccgc cccctgcctg
     1441 gaaatcggca gctactgcgg aaaatcctcc gtctatcttg ggtgcgcctg ccgggaaaac
     1501 ggcgccatat ttttttcagt ggaccatcat accggctcgg aggagcagca gccgggccag
     1561 gaatattttg atcccgagct gctggacaag gaaacgggga aaatcgacac gttccggctg
     1621 tttcgcaaaa cgattgaaga tttcgatctt caggacaccg tcgtcccgat tatcggccgg
     1681 tcggacatca tcggacgggc ctggaagacg ccgcttggcc ttgtctttgt ggacggaagc
     1741 cacgcctacg aatccgttct gaaagactat caagtttggg cggatcacgt ccggtccggc
     1801 ggctatctgg tttttcacga catttttccc aatcccgccg acggcggcca ggcgccctac
     1861 ctcgtttacc aacaggcggt cgcttccggc ctctttgacg cagcgcctct tttcaaatcg
     1921 ctgggggttt tgaaaaagaa ataaaagatt gaacggaaaa aatacagttt agccttatag
     1981 gccgcgcgca gtcatccgaa ggtttcagct ccagcgccag atgcagcccc tgtcgctgaa
     2041 atcggctctc agatagcctt tggcgataaa atcccgcacg gtcgattcaa ccactgcggg
     2101 atcgccggaa atggattcct tcattctttc gatgagcagg ttttccgcct cgtctttgct
     2161 tccgccgccg gccagggatg aatcgatgac gcccaggcgc tccacgaggt cttccttgag
     2221 aatggcatac gtgattttcg tccggctctg cccttcaacc cgcaaatcgt cgtagggggt
     2281 gtttttcccc gtcttgtagt tgtccaccac cttttgccag tcgccgttca cctcggcttc
     2341 atatcgggcg acaaaatccc gaatgtcctg gggcacaagc tccgatgttt tcacgatcgc
     2401 gtcgttggca tcatatttgg accagtcgcg ggtcagaatc tgcagatcat agcgctccac
     2461 tttctcgcac agggtcgtgc ccggaaaagg cgcaaggtaa tggtagccgt acatgatgtc
     2521 cagactccgg gcaaattcat ccgtccgctg aagcgtttct tttgtctctc ccggcagacc
     2581 gaccataaag gaggcgtgcg ccagcattcc ggactgcttg cacattcgga cggcctcggc
     2641 cgcctgcgca agcgttatgc ccttgcgcac ccttttgagc atttccggac agcccgattc
     2701 gacgccgaaa ctgacgctgt cgcagcccac ggacgccatc tcatcaaaca tttcctgcgt
     2761 gacggtgtcc acgcgcgcga aagcgctcca ggtgaatttc aggccgcgct cgcgaatgcc
     2821 ccggcagatt tcccgaacgc ggtttgtgtc cgaagcaaaa aggtcgtcgg cgatgttcat
     2881 ccggtcaaag cccatgccaa tgatctgctc gatctcgtcc accaccaggg ccggattgcg
     2941 gcggcggact ttcgcgccga ccatttttct tcccagacaa aaaa
//
LOCUS       DAOPAF010000114         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_26418_length_2959_cov_44.276171, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000114 DAOPAF010000000
VERSION     DAOPAF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26418_length_2959_cov_44.276171"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLQ77_07915"
     CDS             <1..822
                     /locus_tag="PLQ77_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPL10642.1"
                     /translation="MGSGIAQVAVEAGFQVSLVDTEQGFVDRGMANIRKFLGKKVEKG
                     KIDQARCDEILARLSGTTDMAQAVRGVVMVVEAVFEKFELKKEIFQKLDALCPADVIL
                     ASNTSTLSITSIAAVTKNPRRVIGTHYFSPVPAMRLVEVIRGKETGDAVARKTVEICK
                     AFGKTPIVVKDIPGFIVNRFLCLIYNEAADMINRGDSTAEDIDQAMKLGCNWPMGITE
                     IIDLAGVDVCANAIDAMYQMTGEERYRPSPLFAKMLAEKKLGRKTGEGFYKYDNK"
     gene            861..2081
                     /locus_tag="PLQ77_07920"
     CDS             861..2081
                     /locus_tag="PLQ77_07920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPL10643.1"
                     /translation="MKDREVVLIDGARTAFGRLGGSLKDFTAEELGSIALKGLIDKTK
                     ICERGKVDSVFAGSAFHMARAINPARWIVLDAGLPVTTTASYVEMQCGSAIDGINHAA
                     WKILCGHADVIIAGGMESFSQLPFKFSSSPTPYRIAPPKVLEYQLQPTPKEGRAYLPF
                     EMGLTAENLHEMYNISREEQDQFAFRSQTLAKKAMDEGYFVNEIVTVAVPQGKKKPPL
                     EFKVDEHPRLESLDVLAKLPPAFKKGGTVTAGNSSGLNDGAAFVLLMTAEKAEELGYQ
                     PYARWVCGAEAAVDPSIMGIAPAYAIPIALERAGLKLGDMDVVECNEAFAAQNLAVIK
                     ELEKQTGEKVVMDRWNPLGGAIAFGHANGASGARCALFAMKQLERTGGRYGLFSACCG
                     GGQGVVTIIENLRK"
     gene            complement(2133..2936)
                     /locus_tag="PLQ77_07925"
     CDS             complement(2133..2936)
                     /locus_tag="PLQ77_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL10644.1"
                     /translation="MKDARPVAIVTGSSRGVGAAIAKRLAEKHYSVVINYSKSEREAL
                     EVAAACEALGAETLLCRANVAEDEDCRRMAREAVARWGRIDALVNNAGTTKFCAHDNL
                     EGLVKEDFLNIYSTNLVGPYQMIRAAAPHMKKIGKGAVVNIASTAGITGNGSSVAYCA
                     SKGALINMTKSLARALGPEIRMNAVCPGFIQGEWLEGGMGRENYEGLKHYLEATNPLR
                     MTATAEMIAPAVLFLLEDASLITGEILQLDAGNHLGSAVSVSSRAIPKF"
BASE COUNT          626 a          865 c          891 g          577 t
ORIGIN      
        1 atggggtctg gcattgcgca ggttgccgtg gaagcgggtt tccaggtcag cctggtggac
       61 accgaacagg gatttgtcga tcggggcatg gccaacatcc gtaaattttt aggaaagaaa
      121 gtcgaaaagg gcaaaatcga tcaagcccgc tgcgatgaga tattggcgcg tctttccggc
      181 acaaccgaca tggcccaggc cgtccgggga gtggtgatgg tcgtagaggc cgtttttgag
      241 aagtttgagt tgaagaagga aatctttcag aagctcgatg ccctgtgccc ggccgacgtc
      301 atcctggcca gcaacacctc gacattgagc atcacctcca tcgccgccgt gacgaagaac
      361 ccccggcgcg tcatcggcac ccactacttc agccccgttc ccgccatgcg tttggtcgaa
      421 gtcatccggg ggaaagaaac cggcgacgcc gtcgcccgga agaccgtcga gatctgcaag
      481 gccttcggca agacccccat cgtcgtcaag gacattcccg gcttcatcgt caaccggttt
      541 ttgtgcctga tctacaacga agcggccgac atgatcaaca gaggcgattc caccgccgaa
      601 gacatcgacc aggccatgaa actgggctgc aactggccca tgggcatcac cgagatcatc
      661 gacctggcag gcgtggacgt ttgcgccaac gccattgacg ccatgtacca gatgaccggg
      721 gaggagcgct acaggccgtc gccccttttc gccaaaatgc tggccgaaaa gaaactcggc
      781 cggaagaccg gcgaaggctt ttacaagtac gacaacaaat aaaaacacaa ccgcggccgg
      841 cgcggaaagg gaaaataacc atgaaagaca gagaagtagt attgattgac ggcgcacgga
      901 cggcctttgg ccgcttgggc ggcagcctga aagactttac ggcggaagag ctgggcagca
      961 tcgccctcaa gggactgatt gataagacaa agatttgcga gcgggggaaa gtggacagcg
     1021 tttttgcggg gtctgcgttt cacatggccc gggccattaa cccggcccgc tggatcgttc
     1081 tggatgccgg ccttcccgtg accacaaccg cctcttatgt ggaaatgcag tgcgggtccg
     1141 ccattgacgg catcaatcac gccgcctgga aaatcctttg cggccacgcg gacgtcatca
     1201 tcgccggcgg catggaatcc ttcagccagc tgccgttcaa attttcatcc agcccgacgc
     1261 cttaccgcat tgcgcctccg aaagttctgg agtaccagct tcagccgacg ccgaaggaag
     1321 gccgggccta cctgcccttc gaaatgggcc tcacggcgga aaacctgcat gaaatgtaca
     1381 acatttcacg ggaagagcag gaccagtttg ccttccgcag ccagacgctg gcgaaaaaag
     1441 cgatggacga gggatatttt gtcaatgaaa tcgtaaccgt tgccgttccc caggggaaaa
     1501 agaagccgcc tctggagttc aaagtggatg aacatccccg tctggaatcc ctggacgttc
     1561 tggccaaact gccgcccgcc ttcaaaaagg gagggaccgt cacggcgggc aattcttccg
     1621 gcctcaacga cggcgccgcg ttcgttttgc tcatgacggc cgaaaaagcg gaagaactgg
     1681 ggtatcagcc ctatgcgcgc tgggtctgcg gcgccgaggc ggcggtggat ccttccatca
     1741 tgggtattgc cccggcctac gccattccaa tcgcgctcga gcgggccggc ctcaaattgg
     1801 gagacatgga cgtcgtggaa tgcaacgaag cctttgcggc gcaaaacctg gccgtcatca
     1861 aagagctgga gaagcaaacc ggagagaagg ttgtcatgga ccgttggaat cccctgggcg
     1921 gcgcgattgc ctttggccat gccaacggcg cctccggcgc ccgctgcgcg ctgtttgcca
     1981 tgaagcagct ggagcggacc ggcggtcggt acggactctt ttcggcctgc tgcggcggcg
     2041 gtcagggcgt cgtgacgatc atcgaaaatc tgcggaaata acgggcggaa caatccgacg
     2101 attcagaagt cccggatccg gtttgccgcg gcttaaaatt tcgggatcgc gcgggaactg
     2161 accgagacgg cgcttcccag atgattcccg gcgtcgagct gcaggatttc gccggtgatc
     2221 agggacgcat cctccagcag aaaaaggacc gccggcgcga tcatctcggc cgtggcggtc
     2281 atccggagcg gatttgtcgc ctccagataa tgcttcagcc cttcataatt ttcgcgcccc
     2341 atgccgcctt cgagccattc cccctggata aacccgggac agacggcatt catccggatt
     2401 tccggcccca gcgcgcgggc gagggacttg gtcatgttga tcagagcccc tttggaggcg
     2461 caataggcca cggaacttcc gttgccggtg atgccggcgg tggaggcgat attgacgacg
     2521 gcgccttttc cgattttttt catatgaggg gccgcggcgc ggatcatctg atagggtccc
     2581 acgagattgg tgctgtaaat gttcaggaag tcctccttca ccaggccttc gagattgtcg
     2641 tgggcgcaga atttagtcgt tccggcgttg ttcaccaggg cgtcgattcg tccccacctg
     2701 gcgacggctt cgcgggccat gcgccggcaa tcttcgtctt ccgcgacatt ggcgcggcac
     2761 agcagcgttt cggcccccag ggcttcgcag gccgccgcga cttccagcgc ttccctttcg
     2821 cttttcgagt agttgatcac cacggagtaa tgcttttccg caaggcgttt ggcaatggcg
     2881 gcgccgacgc cccgggagga gccggtgacg atggcaacgg gtcgagcgtc tttcatgaaa
     2941 attctccttc tcctgtttg
//
LOCUS       DAOPAF010000115         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_26419_length_2959_cov_38.851928, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000115 DAOPAF010000000
VERSION     DAOPAF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26419_length_2959_cov_38.851928"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            434..901
                     /locus_tag="PLQ77_07930"
     CDS             434..901
                     /locus_tag="PLQ77_07930"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10645.1"
                     /translation="MKTFTRLEKKAAQILQDDMPLTEKPFAAIADACGLTGDDLLAWI
                     QELSQKGIIRKIGAILRHQKAGYGTNVLVMWSVSPDAIENAGRTLANLPYISHCYERH
                     PAFQNKFNLFTMLHAQHDDISPLLDAMSQLISCNDFLLLESLHEYKKTSPEYF"
     gene            901..2184
                     /gene="hemL"
                     /locus_tag="PLQ77_07935"
     CDS             901..2184
                     /gene="hemL"
                     /locus_tag="PLQ77_07935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPL10646.1"
                     /translation="MKDNPLHPFAAAGRFIAGGVNSPVRAWKSVGGDPVFISHAKGSK
                     IYDSTGKAYVDYVLSWGPMILGHAHPEVIAAICQTAQKGTSFGAPTMAETEMARLICG
                     AIPSIERVRMTSSGTEAAMTAIRLARGYTGRNMILKFDGCYHGHADSLLIKAGSGVTT
                     LGIPGSPGIPEALARLTLSLPYNDADALEKAVEHYAADLAAVIIEPVAGNMGVVPPQE
                     GFLKKLRDLTKENGIVLIFDEVITGFRFTFGGYQNLIGIRPDLTCLGKIIGGGMPVGA
                     LGGKKEIMEKLAPTGDIYQAGTLSGNPLAMSAGIATLRLLERKTDAFASLSEKADRLG
                     CEMENLFSGRGIPVCVNKIYSMFTLFFQEGPVYDLQSALKSDTGLYARFFHGMIQNGV
                     WLAPSQFEAGFLSFAHTDQDMDRTLSALEKTLSRL"
     gene            2288..>2959
                     /locus_tag="PLQ77_07940"
     CDS             2288..>2959
                     /locus_tag="PLQ77_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPL10647.1"
                     /translation="MKKKPLAAQSFYAGKNKIAIVGLGYVGLPLAAHLSRHFNVVGFD
                     ISAANVAELKKGHDRTMELSDAELKKSKIFFTSKPSDLASCKLFIVAVPTPVDHFHVP
                     DLAAMKNASAMIGRHLSPGACVVYESTVYPGVTEDVCVPILERESKLKFGRDFTVGYS
                     PERINPGDKVHTVDNTIKIISASDAATLDLLEGIYSAVVKVRLHRASAIKVAEAAKVI
                     ENTQRD"
BASE COUNT          729 a          815 c          729 g          686 t
ORIGIN      
        1 ggagctgccg ctgccgcttc gggagccgcg aaaacaaaag gagcggaaac aataaccaac
       61 accatcgcca gtatggaata aactaaactc tttttcattg aaatcctcct gattttttat
      121 tgtagcaaat ttaatcaccg gaaccgttaa gcgtctaatt tcttagcctc ccttcttcta
      181 aattttaacg actaccggtc attttcgatt tatctagcga tcaataaact gattgtcaag
      241 aaatattttg actcccggtc cgacaatttt gaaaacaatc atcattccaa gtcttttcat
      301 ggtaaactta tcctgtcatg gtaaacttat cctgtcatgg tgagcccgtc gaaccatgca
      361 ccttttgaca agttcaagat gacggcggtc ttgccttgat tttatttttc aggcaggata
      421 aataagaacc gtcatgaaaa cattcacacg gcttgaaaaa aaagctgcgc aaatccttca
      481 agacgatatg ccgctgaccg aaaagccttt tgcggccatt gccgacgcct gcggccttac
      541 cggcgacgat ctgctggcct ggattcaaga gctttcccaa aaaggcatca tcaggaaaat
      601 cggcgccatt ctgcggcatc agaaggcagg ctatggaacc aatgtcctgg tgatgtggtc
      661 ggtttcaccc gacgccatag aaaacgccgg aagaactttg gcaaatcttc catatatttc
      721 acattgttat gaaagacatc cggcctttca aaacaaattt aatctgttta cgatgctgca
      781 cgcgcagcat gacgacatct cgcctttgct ggacgccatg tcgcagttaa tttcctgcaa
      841 cgattttctt cttctggaaa gtctgcatga atacaaaaaa acgtccccgg agtacttttg
      901 atgaaagaca atcctctcca tccgtttgcc gcagccggcc ggtttatcgc cggcggcgta
      961 aattcaccgg tgcgcgcctg gaaatcggtt ggcggcgatc ctgtttttat aagccatgcc
     1021 aaaggcagta aaatttacga ttccacaggc aaagcatacg tggactatgt gctctcctgg
     1081 ggaccgatga tcctgggtca tgcccatccg gaggttatcg ccgccatttg ccaaaccgcg
     1141 caaaaaggaa caagctttgg cgcgcccacg atggccgaaa cggaaatggc ccggctgatc
     1201 tgcggggcca tcccttccat cgaacgggtc cgcatgacca gttccggaac cgaagccgcc
     1261 atgacggcca ttcgcctggc ccgcggctat accggccgga acatgattct taaatttgac
     1321 ggctgctacc acggacacgc cgattctctt ttaatcaagg ccggctccgg cgtgacgacc
     1381 ctcggcattc ccggctcccc ggggatcccc gaagcgctgg cccggctgac attgagcctg
     1441 ccctacaacg acgcggacgc tctggaaaag gctgtagaac actacgctgc ggaccttgcg
     1501 gccgtcatta ttgaaccggt cgcaggcaac atgggcgttg tgccgccgca ggaaggcttt
     1561 ttgaaaaaac tgcgggacct gacgaaagaa aacggcatcg tcctgatttt cgacgaagtc
     1621 atcacgggat tccgcttcac attcggaggc tatcagaatc tgatcggcat ccggccggat
     1681 ctgacctgcc tgggtaaaat catcggcggc gggatgccgg tgggcgccct gggcggaaaa
     1741 aaagaaatca tggagaaact ggcgccgaca ggcgatattt atcaggctgg gacgctttcc
     1801 ggcaacccgc tggccatgag cgccggcatt gcgacactcc gtcttctaga gagaaaaacc
     1861 gacgccttcg cgtccctttc ggaaaaagcg gatcgtcttg ggtgcgaaat ggaaaatttg
     1921 ttttccggcc gcggcatccc cgtttgcgtc aacaaaattt attcgatgtt taccctgttt
     1981 ttccaggaag gaccggttta tgatttgcaa agcgccctga aatcggacac cggtctttac
     2041 gcccgctttt ttcacggcat gatccaaaac ggcgtctggc tggcgccgtc gcaatttgaa
     2101 gcaggctttc tttcttttgc gcatacggat caggatatgg accggacgct gtccgcgctt
     2161 gaaaagacgc tgagccggct ttaaacaaac tgaacccccg ccagggagag attgacaagg
     2221 cagcatcaat acggtaatgg atttttcttt ttgcatgaaa aaataaaacc gtccaaggat
     2281 ccggaatatg aaaaaaaaac cgctcgcagc tcaatccttc tacgcgggga aaaataaaat
     2341 cgccattgtc ggcctgggtt atgtcggcct gccgctggcc gctcacttat cgcgccactt
     2401 taacgttgtc ggcttcgaca tcagcgcagc caacgtcgcc gagttgaaaa aagggcacga
     2461 ccgcacgatg gagctgtccg acgccgaact gaaaaaatca aaaatttttt tcacgtccaa
     2521 accctccgat ctggcctcct gcaagctttt cattgtcgcc gttcccacgc ctgtggatca
     2581 ttttcatgtt cccgacctgg ccgcgatgaa aaacgcgtcg gcgatgatcg gccggcattt
     2641 atcccccggc gcctgcgttg tttatgaatc aacggtttat cccggcgtca cggaagatgt
     2701 gtgtgttccc atcctggagc gcgaatcgaa attgaaattc ggccgggact ttacggtcgg
     2761 ctattctccg gaacgcatca atcccggcga caaggtccac acggttgaca ataccatcaa
     2821 aataatttcc gcctccgacg cggcgacgct ggaccttctg gagggcatct acagcgcggt
     2881 cgtcaaagtc cgcctgcacc gggcttccgc aatcaaggtc gcggaagcgg ccaaggtcat
     2941 tgaaaacacg cagcgcgac
//
LOCUS       DAOPAF010000116         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_28080_length_2821_cov_63.396602, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000116 DAOPAF010000000
VERSION     DAOPAF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28080_length_2821_cov_63.396602"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLQ77_07945"
     CDS             complement(<1..488)
                     /locus_tag="PLQ77_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPL10648.1"
                     /translation="MAKKIKKVVLAYSGGLDTSVIVRWLIETYGCEVICFAADVGQKE
                     ELTGLRQKALRTGASKIYIEDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAQKERADSVCHGATGKGNDQVRFELTYIALNPRINIISAWKDDHWTFDSRESM
                     FD"
     gene            complement(583..1491)
                     /gene="argF"
                     /locus_tag="PLQ77_07950"
     CDS             complement(583..1491)
                     /gene="argF"
                     /locus_tag="PLQ77_07950"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPL10649.1"
                     /translation="MKKDLLSFAGMQPPDFEEIWQRTVRLKKMLKEGKKHASLEGKTL
                     GMIFDKSSTRTRISFEVGMYQLGGIALFLNSRDTQIGRGETLADSARMMSCYLDGIMI
                     RTFAQESVEELARYATIPVINGLTDLLHPCQILADLFTIQEKKGSYEGVRVAYIGDGN
                     NVANSWIEAATHLPFELALACPEGYDPDSRLMKESRKDGRDRVRLCRDPFEAAAGADV
                     LYTDVWTSMGQEAESQERKKIFANYQINGKLLGVAKKDAIVMHCLPAHRGEEITAEVI
                     DGPQSVVVEEAENRLHAQKAVLEILL"
     gene            complement(1488..2678)
                     /locus_tag="PLQ77_07955"
     CDS             complement(1488..2678)
                     /locus_tag="PLQ77_07955"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPL10650.1"
                     /translation="MDEKKIMTLADQHIMNTYRRVPIALVKGEGARLWDASGREYLDF
                     VAGIAVCNLGHSHPKVVKALKKQAKDLMHVSNLYYTKPQAEVAALLTKHSFADKVFFC
                     NSGAEANEAAIKLARKYAHENLGADKFELITMKDSFHGRTMATITATGQEKFQFGFTP
                     LLDGFKYAPFNDAEALEAAITPRTCGIMLEPIQGEGGVIIPDDRYLAKVRDICNRHGI
                     LMIVDEVQTGIGRTGKLFAYEYSGVKPDIMTLAKALGNGFPVGAMLATDEVARAFSPG
                     NHASTFGGNLLAMAAAKATLKTLIKDGVLDHCQKTGSYFLAKLQALKSRHSFISEVRG
                     RGLMLACAMAIEGADIVKSCQEKGLLINCTGGKTLRFVPPLIVDEKDIDAAISILDEV
                     MEGK"
BASE COUNT          553 a          854 c          734 g          680 t
ORIGIN      
        1 taatcaaaca tggactcccg ggaatcgaac gtccagtggt cgtccttcca ggccgagatg
       61 atgttgatcc tgggattgag cgcgatgtag gtgagctcaa agcgcacctg atcgttgccc
      121 ttgccggttg cgccgtggca aaccgagtcc gctctttcct tctgggcgat ttcaatctgc
      181 cgtttggcga tgagcggccg ggcgagcgac gttcccagca gataggtccc ctcgtaaatc
      241 gcattggcgc gaagcgccgg aaacacgaaa tcccgcgcga attcttcccg caggtcttca
      301 atgtaaattt tggaagcgcc ggtcctgaga gccttttgcc tgagccccgt gagctcttcc
      361 ttctgcccca cgtcggcggc aaaacagatg acctcgcagc cgtaggtttc aatcaaccat
      421 ctcacgatta ccgacgtgtc cagaccgccg gaatatgcca gcaccacttt tttaattttc
      481 tttgccacga tttttcctcc taataatttt caatgtaagc ttcgtgaatt cctttagaac
      541 aaggggttgc aaccccttgt tccagctata cacagaatgt tcctaaagca atatttccaa
      601 aaccgccttt tgcgcgtgca ggcggttttc cgcttcttcc accacaacgg actgcggccc
      661 gtcaatgacc tcggcggtaa tttcctcgcc gcggtgcgcg ggcagacagt gcatcacgat
      721 ggcgtctttc ttcgccaccc ccagaagttt tccgttgatc tggtaattcg caaagatctt
      781 tttccgctcc tgggactcgg cctcctgccc catgctggtc cagacgtccg tatagagcac
      841 gtccgctccc gccgcggctt caaacggatc gcggcacagc cggacgcgat cccgtccgtc
      901 tttccggctt tccttcatca ggcgagaatc cggatcgtag ccctcgggac aggccagcgc
      961 cagttcgaaa ggcaggtgcg ttgccgcttc gatccaactg ttggccacat tgtttccgtc
     1021 gccgatatag gcaaccctga cgccttcata ggatcctttt ttctcctgaa tcgtaaacag
     1081 atccgccaga atctggcagg gatgcagcag gtccgtgagc ccgttgatga ccggaatggt
     1141 cgcgtagcgg gcaagttctt ccacgctttc ctgggcaaag gtccggatca tgatgccgtc
     1201 gagatagcag gacatcattc gggccgaatc cgcgagcgtt tcaccgcgtc cgatctgcgt
     1261 gtcccgggaa ttgagaaaaa gcgcgatgcc gcccaactga tacatgccca cttcaaagga
     1321 tatccgggtt cgcgtggagg atttgtcaaa gatcatgccc agcgtcttgc cctcaagcga
     1381 cgcgtgcttt ttgccttcct tcagcatttt tttgaggcgg accgtccttt gccagatttc
     1441 ttcaaaatcc ggcggctgca tgccggcaaa gctcagcaga tcttttttca tttcccctcc
     1501 atgacctcat cgagtatgga aatcgcggcg tcaatgtctt tctcatccac aatcagcggc
     1561 ggaacaaaac gcagtgtttt gccgccggtg cagttgatga gcagcccctt ttcctgacag
     1621 cttttgacga tgtctgcgcc ttcgatggcc atcgcacagg ccagcatgag ccccctgccc
     1681 cgcacttcgg aaataaaaga atgccgcgac ttcagggcct gaagtttggc caggaaatag
     1741 cttcccgttt tttgacaatg atcgagcacg ccgtctttga tcagggtctt cagcgtggct
     1801 ttggccgccg ccatcgccag caggttgccg ccgaaggtgg aggcgtgatt gccgggcgaa
     1861 aaggcgcggg ccacttcatc ggtcgccagc atggcgccta ccggaaagcc gttccccagc
     1921 gccttggcca gcgtcatgat gtccggtttg acgccggaat attcataggc aaacagcttg
     1981 cccgtccggc cgatgccggt ctgcacctca tcgacgatca tcaaaattcc atggcgattg
     2041 cagatgtccc gcaccttcgc cagataccga tcatcgggaa taatcacgcc gccttccccc
     2101 tgaatcggct ccagcatgat tccgcaggtt cggggagtaa tggccgcctc cagcgcttcc
     2161 gcatcgttga acggggcgta tttaaagccg tcgagaagcg gcgtgaatcc gaactgaaac
     2221 ttttcctggc ccgtggcggt aatggttgcc atcgtccggc cgtgaaacga gtctttcatg
     2281 gtgatgagtt cgaatttatc cgcgcccagg ttttcatggg cgtattttcg cgccagcttg
     2341 atggccgcct cgttggcttc cgcgccgctg ttgcagaaaa aaaccttgtc ggcaaaggaa
     2401 tgcttcgtca gaagagcggc gacttccgcc tggggcttcg tgtaataaag gttggagacg
     2461 tgcatcagat ccttggcctg ctttttcagc gctttgacca ccttcggatg ggaatgcccc
     2521 agattgcaca cggcgatgcc ggccacaaaa tccagatatt ccctgccgct tgcgtcccag
     2581 agcctcgcac cctcgccttt gaccagcgcg atcggcaccc gccggtaggt attcataatg
     2641 tgctgatcgg ccagcgtcat gatctttttt tcgtccatgc tttgttcccc tttgaggcgt
     2701 tatacaacga tttccgttcc gattcccata tccgtaaaga cttccagcag gatcgcgtgc
     2761 ttcagccgcc cgtccacgat atgcgccttt ttcacgccgc cgcgcagcgc cttgaggcag
     2821 c
//
LOCUS       DAOPAF010000117         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_28294_length_2805_cov_35.896727, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000117 DAOPAF010000000
VERSION     DAOPAF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28294_length_2805_cov_35.896727"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1014
                     /locus_tag="PLQ77_07960"
     CDS             205..1014
                     /locus_tag="PLQ77_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPL10651.1"
                     /translation="MTQLDLNTLFSLKGKIALITGGSRGIGRMIAQGFLQAGAKVYIT
                     ARKKADCEETAKELSAFGPCIAIPSDIGTAQGRAALLKAFSEKEDKLHILINNAGTNW
                     GNVFDQYPDEAFEKVLQMNVSAVFSMTRDFVRLLENAATPEDPARVINVGSMDGLHIP
                     TVSRVGTFAYTSSKAAVHHLTRNLAVTLAPRFIAVNAIAPGFFPSRMTDYIFDKYKPD
                     IEQNALLKRVGKPEEMAAVAIYLCSKGGAYTDGAVIVVDGGTSINHQHVRT"
     gene            complement(1103..2635)
                     /locus_tag="PLQ77_07965"
     CDS             complement(1103..2635)
                     /locus_tag="PLQ77_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPL10652.1"
                     /translation="MHAVLLRVILVSIILTMCVPWSLAVASEQRMALVIGNSAYSTGP
                     LRNPVNDAADMAAALQKLGFEVILKKNATLQEMEESVFHFADRLKKGGVGLFYFAGHG
                     VQVNGENYLLPVGANISKETDVKYKAVNANRIMDEMAYANNGLNVVLLDACRDNPFAR
                     TFRSTSRGLAIVNSAPAGTFISFSTGPGQVARDGEGRNSPYTAALLKYMQIPGLTISD
                     VFINVRTRVKQETGQIPWELSSLEGQFYFVPDSQRKTVDPSGMDAALEKQNRQESETQ
                     PSPAASEKPSAFTDLKKKMEERRERENQLSQDFEAAKKLEKESLDITERIQVWKHFTA
                     ALPDERTARIDELRKYAMKRIENLERYKATGQNLPREMKKDKRFIAYDDGTVLDTKTN
                     LMWAAKDNGSNLNWTNAKAYCENYRGGGYSDWRMPTQTELSELHDAHKIYPSDCRGPF
                     GGTWPLYYATDLIHLSCMGQWAAEASGSKAAYFHFNVGVKGWAPQSDTIYNRALPVRS
                     AE"
BASE COUNT          653 a          752 c          713 g          687 t
ORIGIN      
        1 atttctgccg cgtcttttgt tccacaaagt tagaaatgag acaaaaaagc gggggcgaac
       61 aacatcgaat actcacgaat tctttgcgac aaccagacgg cggatacatc tttgttgaaa
      121 gaatccccga aatgagatgg ccgtcttcga aactactatt ggcaaacaaa cggtcttcat
      181 cgaacaaata taaggaggag gcatatgact caactggatc tcaatacgtt gttttcgctg
      241 aaaggaaaga ttgctctgat cacgggcgga tcgaggggca tcggcaggat gatcgcccag
      301 ggatttctcc aggccggcgc aaaggtttat atcacggcca gaaagaaagc ggactgcgag
      361 gaaacggcaa aagagctctc cgccttcggg ccatgcatcg cgattccatc cgacatcggc
      421 acggcccagg ggcgcgcggc gctcctgaaa gcattttctg aaaaggaaga caagctccac
      481 attctgatca ataacgccgg aacgaattgg ggaaacgtct tcgaccaata cccggacgaa
      541 gcttttgaaa aggtgctgca gatgaacgtc agcgcggtct tttccatgac gcgggatttc
      601 gttcgtcttc tggaaaacgc ggcgacgccg gaagatccgg ccagggtaat caatgtcgga
      661 tccatggatg gtctgcatat tccgacggtc agccgtgtcg gaacatttgc ctacacctcg
      721 agcaaggcgg cggtgcatca tctgacgcgg aatctggcgg tgacgctggc gccgcgtttc
      781 attgcggtca acgcgatcgc ccccggcttt ttccccagtc gcatgaccga ctacatattc
      841 gacaaatata agccggacat cgagcaaaat gccctgctga agcgcgtggg aaaaccggag
      901 gaaatggcgg cggtggcgat ctatctgtgc tccaaaggcg gcgcctacac cgacggcgcg
      961 gtgatcgtcg tggacggcgg cacgtcgatc aatcatcagc acgtgagaac ttgagaaaat
     1021 cgttttgacg ctacgccctc aagtggcaaa agccgtgcgg ctgacgctac cggtccgatg
     1081 gaaaaggctc ccaaccctgc atctattcag cggatctcac cgggagggca cggttgtaaa
     1141 tggtatccga ctggggcgcc cagcctttaa caccgacgtt gaagtggaaa taagcggcct
     1201 tcgaaccgct ggcttcagca gcccattgcc ccatacagct aaggtgaata aggtctgttg
     1261 cataataaag cggccatgta ccgccgaaag gtcctctgca atcggaagga taaattttat
     1321 gcgcgtcatg cagttctgac agttccgtct gcgtgggcat gcgccaatcc gagtatccgc
     1381 cgccccgata attttcgcag taagccttcg cgtttgtcca gttgagattg cttccgttgt
     1441 ctttcgcagc ccacatcaaa tttgtcttcg tatccaatac cgtcccgtcg tcatacgcaa
     1501 taaacctttt gtctttcttc atttctctgg gcagattctg acccgttgcc ttataccgtt
     1561 ccaggttttc tatccgcttc atcgcatatt tcctgagttc gtcaatcctg gcggttcgtt
     1621 cgtcgggcag cgccgccgta aaatgtttcc acacctgaat tctctccgtg atgtccaggc
     1681 tttccttctc cagctttttt gccgcttcaa aatcctgcga cagctggttt tctctttccc
     1741 gtctttcctc cattttcttt ttcagatcgg taaaggcaga aggtttttca gacgctgccg
     1801 gactgggctg tgtttccgat tcttgccggt tctgcttttc cagcgccgcg tccatcccgg
     1861 atggatcaac cgttttcctt tgcgaatccg gcacaaaata gaactgcccc tccagagagg
     1921 acaactccca gggtatctgc cccgtttcct gctttactct tgtgcggacg ttgatgaaga
     1981 catcgctgat ggtcagtccg ggaatctgca tatacttgag caatgcagcg gtgtaagggc
     2041 tgttgcgccc ttcgccgtct cttgccacct ggcccggtcc tgtggagaag gatataaacg
     2101 tcccggcggg ggcgctgttc acaatggcca gtccgcggga ggtgctgcgg aacgttctgg
     2161 cgaagggatt atcacggcag gcgtccagca atacaacatt aagaccgttg ttggcgtacg
     2221 ccatttcatc catgattctg ttggcattaa cggctttgta ttttacgtcc gtttctttgc
     2281 tgatattggc tccgacagga agaaggtaat tttcaccatt gacctgcaca ccatgaccgg
     2341 caaagtagaa caaacccacg cctccttttt tcagtctgtc ggcgaaatgg aaaacggatt
     2401 cttccatttc ctgaagcgtc gcattcttct tcagaatcac ttcaaaaccc agtttctgca
     2461 gtgctgcggc catatcggct gcgtcattga cggggtttct gagcggaccc gtactgtagg
     2521 cgctgttgcc gataaccaaa gccatgcgct gttccgaggc caccgccagc gaccatggaa
     2581 cacacatggt caggatgatc gatacaagga tgactctcaa caagacggca tgcacgggaa
     2641 ttttctcctt tgcatggatt ttttttgatt atacatcatg aaaaactcaa tgacaaagag
     2701 aatataccca aaccgtgtcc cggtaaccaa aacagagata ccgcctggac gcgacctgtt
     2761 atcggagcat ttggtacata aaggcattgg atcattacaa ggatt
//
LOCUS       DAOPAF010000118         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_29689_length_2701_cov_44.649282, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000118 DAOPAF010000000
VERSION     DAOPAF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29689_length_2701_cov_44.649282"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLQ77_07970"
     CDS             complement(<1..1370)
                     /locus_tag="PLQ77_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL10653.1"
                     /translation="MEQPWFKQYAKHVSRTLQYPNAPVYAFLEKTAAAHPEYIAMTFN
                     DADTTYAELARKVNRFAAVLNGQGVKKGDRVALILINSPTYVISFFAILKLGAVAVNL
                     SVGITGEELAACLINSGARMAITLDLFAKNLYRVISKTSVKNVILHSVFGMEKKIPLT
                     EGMPAPQIFSDLMAGAGDAAGPDVEVLTTDVAVLQYTSGSTGAPKAATLTHANLVAST
                     TQSDKWMGHEHTGNAAVLCVIPFFHVFGMTAGLLIPVSKGYRMLLLPRMDAMDILSMT
                     KMLETHRPISFPAVPSLWQAIVSLPDETARDYLSSILVATSGGAPAPAALHDRYKSLT
                     GKRMIEAYGLSEASSATHMSPYPKGGPVGSVGVPLPDTQAKIMDLETGQRECEPGEIG
                     ELVVRGPQIMRGYWNNPELTAAVLQDGWFYTRDLARMDENGFFYLIDRKDDMIISRGF
                     NVYPAQ"
     gene            complement(1395..>2701)
                     /locus_tag="PLQ77_07975"
     CDS             complement(1395..>2701)
                     /locus_tag="PLQ77_07975"
                     /inference="COORDINATES: protein motif:HMM:NF039222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPL10654.1"
                     /translation="RLYPINPKATEVLGLKAYPSLRALPEVPDLVMVAVRADLVPAAL
                     TECADVGARFIHVLTAGFSEIGTPEGRELEARLAAIAKERNLRIIGPNCMGPYSPSSH
                     LTAWGGIPGLDGRLGIISQSGGVTQRFTEYISSLGVGVSKAASIGNAAVLDSPDFLEF
                     MAGDSDVDLIAVYLESIRDGRRFFDLARRVTPRKPIILLKGGESRRGAATVSSHTGRM
                     AGDPVIWKSVFEQTGIVPVTNLQEWVDACLAFSLLPKTRGKGVFIVGGGGGNSVIFSD
                     ACIRAGLEVPTLTDRSMGRIRPFVPVAGSIAGNPLDFWGTYLVNDFLFEALNAAYEDD
                     SIHMVVVDRLIPRIAFHSPDLTSDSFAEIVEFIQSRRQAKPTVFTIDYDGGDSDLIRQ
                     GSDLRARFCRAGIPAYPSFERAIGALARFYRYCRRFGHAAGR"
BASE COUNT          568 a          831 c          778 g          524 t
ORIGIN      
        1 atctgggcgg gatagacgtt gaatcccctg gaaatgatca tgtcgtcctt gcggtcgatc
       61 agatagaaaa atccgttttc gtccatgcgg gcaaggtcgc gcgtgtaaaa ccacccgtct
      121 tgcaaaacgg cggcggtgag ctccggattg ttccagtagc cccgcatgat ctgcggcccc
      181 ctgacaacca gctccccgat ctcacccggt tcacattccc tttgaccggt ttccagatcc
      241 atgattttcg cctgcgtgtc cggaaggggc acgccgaccg atcccaccgg cccgcctttc
      301 ggatagggcg acatgtgcgt cgcggaggac gcttcggaaa ggccgtaggc ttcgatcatc
      361 cgctttccgg tcaggctttt gtagcggtca tggagcgcgg ccggcgcggg cgctccgccg
      421 ctggtggcca ccagaatgga ggaaaggtag tcccgggccg tctcatcggg aagcgaaacg
      481 atcgcctgcc agagactggg cacagccgga aacgatatcg ggcggtgggt ttccagcatc
      541 ttcgtcatgg acaggatgtc catcgcgtcc attcggggaa gcagcagcat tcggtatccc
      601 ttgctcaccg gaatcagcag gccggccgtc attccaaaga cgtgaaaaaa tggaatgacg
      661 cacagcaccg ccgcgttccc cgtgtgttcg tggcccatcc atttgtccga ttgcgtcgtg
      721 ctggcaacaa gattggcatg cgtgagcgtg gccgccttgg gtgcgcccgt ggagccgctg
      781 gtgtattgca gcacggcgac atccgtggtt aagacctcaa cgtccggccc ggccgcatcg
      841 cctgcgccgg ccatcaaatc ggaaaagatc tgcggggcgg gcatgccctc cgtcagggga
      901 atctttttct ccattccgaa cacggaatga agaatgacgt ttttcacaga ggttttggaa
      961 atcacccggt agagattttt ggcaaacaga tccagcgtaa tggccattct cgcgccggaa
     1021 ttgatgaggc aggcggccag ttcctcgccc gtgatgccga cgcttaagtt gaccgcgacc
     1081 gcgccgagtt tcagaatcgc gaaaaaagaa atgacgtagg taggcgaatt gatcagaatc
     1141 aaagcgacgc ggtctccttt tttcacgccc tgcccgttta gaacggcggc gaaccggttg
     1201 accttccggg caagttcggc gtaggtcgta tccgcgtcgt tgaacgtcat ggcgatatat
     1261 tccggatgcg ccgccgccgt cttttccaga aatgcataaa ccggcgcatt cggatactga
     1321 agcgtccggg acacatgctt cgcgtattgt ttgaaccagg gctgttccat gttttcttcc
     1381 tttccaaaac gggactatcg gccggccgcg tggccaaagc gccggcagta tcgataaaag
     1441 cgcgccagcg cgccgatggc cctttcaaac gacgggtagg ccggaatccc cgctctgcag
     1501 aaccgggcac gcagatcgga accctgccga atgaggtcgg aatcgcctcc gtcgtaatca
     1561 atggtgaaga cggtgggctt ggcctgcctg cggctttgaa tgaactccac aatctcagca
     1621 aacgaatccg aggtcaagtc cgggctgtgg aaagcgatgc gcgggatcag gcggtcgacg
     1681 accaccatgt gaatgctatc atcctcgtag gccgcattga gcgcctcgaa aagaaaatca
     1741 ttcaccagat aggttcccca gaaatcgagg ggattcccgg caatacttcc ggccaccggc
     1801 acgaagggcc gaatcctgcc catgcttcgg tccgtaagtg tcggcacttc cagacccgcc
     1861 cgaatgcacg cgtcggaaaa aataacactg tttcccccgc cgcctccgac aataaacacg
     1921 cctttccccc gggttttcgg cagcagggaa aaagccaggc aggcatccac ccattcctga
     1981 aggttcgtga cgggaacaat gccggtctgt tcaaaaaccg atttccagat cacgggatcg
     2041 cccgccatcc gtcccgtgtg agatgaaacg gtcgccgctc cccggcgcga ttccccgcct
     2101 ttaagaagaa tgatgggttt ccggggtgtc acgcgcctgg cgagatcaaa aaagcgccgt
     2161 ccgtcccgaa tgctttccag ataaacggcg atcagatcga catcggaatc gcccgccatg
     2221 aactccagaa aatccgggct gtccagcacg gcggcgttcc cgatgcttgc cgccttgctc
     2281 accccgacgc ccagcgagct gatatattcc gtgaatcgct gcgtcacgcc gccgctctga
     2341 gaaataatcc ccaggcggcc gtctagcccc ggaataccgc cccaggccgt cagatggctg
     2401 gatggactgt aggggcccat acagttcggc ccgatgatcc gaagattgcg ctctttggca
     2461 atggcggcaa gccgcgcctc cagctcccgc ccttccggcg tgccgatctc gctgaatccc
     2521 gccgtcagga catgaatgaa acgcgctccg acatcggcgc attccgtaag cgcggcgggc
     2581 acgaggtcgg cccggacggc caccatcacc agatccggca cctccggcag ggcgcgaagc
     2641 gacggatagg ctttcaaacc cagaacctcc gtcgccttcg ggttgatcgg ataaagacgc
     2701 c
//
LOCUS       DAOPAF010000119         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_31191_length_2600_cov_31.610609, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000119 DAOPAF010000000
VERSION     DAOPAF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31191_length_2600_cov_31.610609"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1944
                     /locus_tag="PLQ77_07980"
     CDS             289..1944
                     /locus_tag="PLQ77_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPL10655.1"
                     /translation="MKTYADRPWLAHMDPRIDRNPILPETPVHQFLTDSFRKYPDHIA
                     LYFYGAEFTYAELESLTNKLANSLAKLGVRKGDRVALCMDNCPQYVIAFFAVLKAGAA
                     VVQITPMATEREALYLINDSGSKGIVTLDYIWPRIKTVMNESPLAFAVVGSLYDYLPL
                     TPFPVKPFAIPEKPLGIESAADVYEFKTLLNESCAFTPPVMNSRQDLAVLQYTSGTTG
                     FAKGVMVTHYNLSSYVTLLMTMDYRNEPGKEIYPVTLPMSHNYAMFQTVVAPIAMGGK
                     VVIMVRFHPDEALRVIDRLHTTIFRAVPTMLTMMIAHPRLAEFNLRSVRHWVVGGAPV
                     PDELVAKFQQVSGANVVEGYGLTESTSGVVLNSLYDKTHKGMGFPAIMMDARVINPET
                     GEDVPFGTDGELLLKGPAISPGYWNKPEETSKTFEGGWLHTGDIVRMSEQGVLQFVDR
                     LKEMIIVSGFNVYPTEVENALYEHPGVMEAAVIGWPDERQGEIVKAVIVPRKDAVLKA
                     DEMISFCKERLTAYKVPKIIEVVDSLPKNPTGKIQKKALQKKV"
     gene            1975..2202
                     /locus_tag="PLQ77_07985"
     CDS             1975..2202
                     /locus_tag="PLQ77_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10656.1"
                     /translation="MPQTRMFHSQALAILDSLHRFGLRELFAHAVLAHEFIFRKILKE
                     QREQTQEAGTGAAAPTAWSGTAMAVEGKIAE"
BASE COUNT          621 a          690 c          717 g          572 t
ORIGIN      
        1 tattttgcgt ctggttaaca caaccaggtc tcataatcaa cgaaaaggtc atccgcgcgg
       61 ggccagcttt gccccttccc tccttttccg accatgcaga tcggcgcctg atttctcaat
      121 ccacaagcat tgacaatcgc ccggcttcca tgttatgcgg ctcgaagatt ctcgcggaaa
      181 atattttgtt tggacaggat gaatcgtttg cgcgtacggc gtgccgtgaa acccctgaga
      241 gggttccaat gctcgccaca accccaaaat gcattatgga gggcctttat gaaaacgtat
      301 gccgacagac cgtggcttgc acacatggat ccccgaattg accgtaatcc gattttgcct
      361 gaaacgccgg tccatcagtt cctcaccgat tctttccgaa aatatccgga ccacattgcg
      421 ctgtattttt acggcgccga gtttacctac gcggagctgg aaagcctgac gaacaagctg
      481 gccaacagcc tggcaaagct gggcgtcagg aagggcgacc gcgtggctct ctgcatggac
      541 aattgtccgc agtatgtcat cgcctttttt gccgtgctca aggcgggtgc ggctgttgtg
      601 cagatcacgc ccatggcaac ggagcgggaa gcgctttatc tgatcaatga ttccggaagc
      661 aaaggcatag tgaccctgga ttatatctgg ccaaggatta agaccgtgat gaacgaaagt
      721 ccactggcct ttgccgtcgt cggcagcctc tatgactatc tcccgttaac tcctttcccg
      781 gttaaaccct ttgccattcc cgaaaaaccg ctggggattg aatccgccgc cgacgtctat
      841 gagttcaaaa cgctgctcaa cgagtcctgt gcgtttactc cgccggtcat gaacagtcgc
      901 caggatctgg cggttttaca gtataccagc gggaccaccg gttttgccaa aggcgttatg
      961 gtgacccatt ataatttgtc ttcttacgtt acgctgctga tgaccatgga ttacagaaat
     1021 gagccgggaa aggaaattta tccggtgacc ctgcccatgt cccacaatta tgccatgttc
     1081 cagacggtcg tcgcgcccat cgccatgggc ggcaaggttg taatcatggt gcgctttcat
     1141 ccggatgaag ccctcagagt aatcgaccgt ctccatacca cgattttccg ggccgtgccc
     1201 acgatgctga ccatgatgat cgcccatccc cgccttgcgg agttcaacct ccgttccgtc
     1261 cgccactggg ttgttggagg tgcgccggtc cccgatgaat tggtggcaaa atttcagcag
     1321 gtgtccggcg cgaatgtcgt cgaggggtac ggtctgacgg aatcgacttc cggagtggtc
     1381 ctgaattcgc tgtacgacaa gacgcacaaa ggcatggggt tcccggccat catgatggac
     1441 gcccgcgtca tcaatcctga aaccggcgag gatgttcctt tcgggaccga tggagaactg
     1501 ctgctgaaag gcccggccat cagccccggc tactggaaca agcccgaaga aacgtcaaaa
     1561 acatttgagg gcggatggct tcataccggg gatatcgtgc gcatgtcgga acagggcgta
     1621 ttgcagtttg tggaccggct caaggaaatg atcatcgtct ccggtttcaa tgtttatccc
     1681 acggaagttg agaatgcgct gtatgaacac cccggcgtga tggaagcggc ggtgatcgga
     1741 tggccggatg aacggcaggg ggaaattgtc aaagcggtga ttgtgccccg aaaggatgcc
     1801 gtcctgaagg ccgacgagat gatcagcttc tgcaaggagc ggctgaccgc ctataaagtt
     1861 ccaaaaatca tcgaggttgt ggacagtttg ccgaagaatc cgaccggcaa gattcagaag
     1921 aaagccctgc aaaagaaagt gtaggtttcg caaagcaaag gagccggcca tcgaatgccg
     1981 caaacccgga tgttccattc gcaggctttg gccatattag acagccttca tcgattcggg
     2041 ttgcgtgaat tgtttgcgca tgccgtcctg gcccatgaat tcattttccg taagatctta
     2101 aaggagcaac gggaacagac acaggaggcc ggaacagggg ccgcagcgcc gaccgcctgg
     2161 tccggcactg caatggccgt cgaaggcaag attgcggaat agaaacgctt aagaaacaca
     2221 ggggtttata acgaaggggg cgcctgaaag aggcggcggc cggaattcct gatgatcccg
     2281 tcgagatgga ctacattccc gccatgcaat ggtcgccgga ttgggaactg atcaagggga
     2341 caaaatcatc gtgaatcccg acaagagcgc cggggcaaac catctttctc tggaccctga
     2401 tgccatcatc gtcgcaagtc agcataaaag gatcgtcgcc taattcctca atacacaccc
     2461 gcgaacaatc gccccatgcg gatttcttga ccccaaagca agtatagggt agagatcatt
     2521 cgacaaacgg gaaagattgc cgccggtttt cgggaggatg agatgaaaag gaaatgggct
     2581 gtccttttgg tggtgatggc
//
LOCUS       DAOPAF010000120         2595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_31277_length_2595_cov_62.601575, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000120 DAOPAF010000000
VERSION     DAOPAF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31277_length_2595_cov_62.601575"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1843
                     /locus_tag="PLQ77_07990"
     CDS             290..1843
                     /locus_tag="PLQ77_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPL10657.1"
                     /translation="MKEDDVEKKSISRRNFLKTAGGAAAVAGLASSGIPLMVGTAQAA
                     CDALPKKWDETYDVVVVGSGFAGLAAAIEAKNAGASVALLEKMPVHGGNSVINGGDFC
                     APGTKLQKENGVEDSADLLYKDMMRAGMYLNHPELARTVADNAKDALEWTESYLGCRY
                     TKLNFHGGHSVKRAHGTINQSGSEVVNKELAKAKEIGVKIMLRTKLVKFLSDKHGRIV
                     GMEVLKGYRFPDDKSGKTAFLKAKKAVVLTSGGFSQDIALRQVHDPRLTDKLGSTNHP
                     GATGEALLAACASGAADVQMDWIQLGPWTSPEEKGFGYVPLFCEPLVGYGLMVNPKTG
                     KRFFKETGNRKERADAILLLGNPAVIMGDSYAVNKQVVPHALKGGLENGAIKKFDSLD
                     DLARAYGMPVDAFKEQVARWNSFAEKRKDDDLGCMIFKDAKPTVEAPFYAARLWPKVH
                     HTMGGLVINKDAQVMGFDFKPVKGLYAAGEVTGGVHGAVRLGSVAMADCIVFGRIAGK
                     NAAREKAWS"
     gene            1918..2436
                     /locus_tag="PLQ77_07995"
     CDS             1918..2436
                     /locus_tag="PLQ77_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPL10658.1"
                     /translation="MERKKNLKIAAVLFGAVLLVAVSTGLWAAPKLTVSKAKPGDCAA
                     CHADKKVLPETHPDTRSMDMKACRACHTVDNMKLSGKFPASHTHMLSGMSCEACHGKT
                     KSPEEVPMEKCVACHGPTAKLAEKTKDVKPTNPHTSPHYGTELDCNLCHKQHAKSENY
                     CAQCHKFDFKTP"
BASE COUNT          624 a          679 c          779 g          513 t
ORIGIN      
        1 atggatggaa agtttcccct ttccgataac ggaaacgggc cggtgtttgc ctgcctcaac
       61 caaaagggaa aagtgccgga ttgccgcatt cctcggtgat aattgccgtt gacacttttc
      121 ttcccgccgg tgtataaatt caccgcgcga tctttgcgtt tcatcgcttt tcattggatg
      181 acagggtcat cgaatccggc tttgacggga gcttgtgccc gcattcttat gccgggtgaa
      241 gtgttttcag gaacattcgg caaaacaata tcatttaagg aggaaaacca tgaaagaaga
      301 tgacgttgaa aaaaagtcga tcagcaggcg gaatttttta aagaccgccg gcggggcggc
      361 ggccgtcgcc ggactggcct ccagcggaat ccccctgatg gttggaaccg ctcaggcggc
      421 ctgcgacgcg ttgcctaaaa aatgggatga aacctacgat gtggtggtgg tgggcagcgg
      481 ctttgccggc ctggccgcgg ccattgaagc gaaaaacgcg ggcgccagtg ttgccctgct
      541 ggagaaaatg ccggtgcacg gcggcaattc cgtcatcaac ggcggcgact tctgcgctcc
      601 gggaacgaag ctgcaaaagg aaaacggcgt agaggattcg gcggatctcc tgtataaaga
      661 tatgatgaga gccggaatgt acctgaatca tccggagctc gccaggaccg ttgccgataa
      721 cgcgaaagac gctctggagt ggaccgagtc ctatctgggc tgcagataca cgaaactgaa
      781 ttttcatggc ggccattcgg tcaaaagagc gcatggaacg atcaatcaat ccggttcgga
      841 agtggtcaat aaggaactcg cgaaagccaa agagattggc gtaaaaatta tgctgcgcac
      901 caagctggtg aaattcctgt ccgataaaca tgggcgaatc gtcggtatgg aagtgctcaa
      961 aggctaccgg tttccggatg acaagtccgg caagaccgcc ttcctcaaag caaagaaggc
     1021 cgttgttttg acctccggcg gattctccca ggatatcgcg ctccgtcagg tccatgatcc
     1081 acggctcacc gataagttgg gatcaaccaa ccatcccggg gccaccggcg aagcgctgct
     1141 ggccgcctgc gcaagcggcg cggcggatgt tcagatggat tggattcagc tcgggccctg
     1201 gaccagtccg gaagaaaaag gtttcggcta tgttcctctg ttctgtgaac cgctggtcgg
     1261 ttacggtctg atggtgaatc ccaaaaccgg gaagcgtttt ttcaaggaaa ccggaaaccg
     1321 caaggaacgc gcggatgcca ttcttctctt gggaaatccc gccgttatca tgggcgattc
     1381 ttacgcggtc aacaagcagg tcgttcctca tgcgctcaag ggaggcctgg aaaacggagc
     1441 gatcaagaaa tttgactctc tggacgatct cgccagggct tatggcatgc cggtggacgc
     1501 attcaaggaa caggttgccc gctggaattc ctttgcggaa aaaagaaagg atgacgattt
     1561 gggatgcatg atcttcaaag acgccaaacc gaccgtcgag gcgcctttct atgccgcgcg
     1621 gctgtggccg aaggttcatc acacgatggg tggactggtg atcaataaag acgcccaggt
     1681 gatgggattc gacttcaaac cggtaaaagg tctttatgcc gccggagaag tgacgggagg
     1741 cgttcatggc gccgtgcgac tgggaagcgt cgccatggcg gattgtattg tcttcggccg
     1801 catcgccggc aaaaacgccg ccagggaaaa agcctggagt tgaaaaagcc caatccgcac
     1861 gcggacggtt tccggatgtc cgcgcgcgga ttgaaatgag gcaaggagaa aagtcgtatg
     1921 gaaagaaaga aaaatctgaa aatcgcggcg gtcctgttcg gcgcggtttt gctggttgcc
     1981 gtgagcacgg gactgtgggc ggcgcccaag ctgaccgtgt ccaaggccaa accgggcgat
     2041 tgcgctgcct gtcatgccga taaaaaggtg cttccggaga cgcatcccga cacccggtcg
     2101 atggatatga aggcctgccg ggcctgtcac accgtggaca atatgaaact gagcgggaaa
     2161 tttcccgcca gccacacgca tatgctctcc ggcatgagct gcgaagcctg tcacggcaag
     2221 accaagtcgc cggaagaagt gccgatggag aaatgcgtcg cctgccacgg gccgacggca
     2281 aagctggcgg aaaaaacgaa ggatgtgaag ccgaccaatc cgcacacgtc cccgcattac
     2341 ggaacggagc tggactgcaa cctgtgccac aagcagcatg ccaaatcgga aaactactgc
     2401 gcgcagtgcc acaagttcga cttcaagaca ccgtaaatta cccctgaatc ttcaccccgg
     2461 accggcggcg ggcctgacga ctgccgccgg tccgtttttg ttgaggagga acagaggatt
     2521 gccggtcctg tttaacctgg cgcgcgttga gtcccgctta agcgggacga gcgttcattc
     2581 tccgcggctt gccgc
//
LOCUS       DAOPAF010000121         2440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_33934_length_2440_cov_47.610063, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000121 DAOPAF010000000
VERSION     DAOPAF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33934_length_2440_cov_47.610063"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLQ77_08000"
     CDS             complement(<1..769)
                     /locus_tag="PLQ77_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPL10659.1"
                     /translation="MEVFQRLFTMARQYYARFILAAICMIVAGGLQSALPLIAKPAID
                     EIFMSKNIAALKWIPLAVVAIFLFKGLANYGQNILMSSIGLRIVTNLRNQLYEQIQKQ
                     SLSFFAEHPTGLLMSRITNDVQSVQTASSEAITALVKDTFMLICLVGVVFYTDWKLAL
                     IAMVVFPLTIYPISRFGKKMRKVTTSSQITMGTLNSLLQETISGTRIVKAFCMEKYEN
                     DRFAAENERLFKYSMKAVSVNAISSPLMDFLGGLGIAA"
     gene            complement(778..1929)
                     /gene="lpxB"
                     /locus_tag="PLQ77_08005"
     CDS             complement(778..1929)
                     /gene="lpxB"
                     /locus_tag="PLQ77_08005"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPL10660.1"
                     /translation="MNKNSGSAPRVMIVAGEASGDLHGALLVREMLAVNPSVEFFGIG
                     GNQMREAGVKLLAHAADIAVVGVSEVFSKTAVLLRTISRMKKSLDRLKPNLVILIDFP
                     DFNLNIAARAAKKRGIRVFYYISPQVWAWREGRVEQIKKLVDKMAVILPFEVDFYAAH
                     GFDVHWVGHPLLDIVKTTFTAQEARAHFGLSANKTTIGLLPGSRNSEIQKLLPEMIQA
                     AQIISQKFPTAQYILPLAETLEQKTVAGFLQGSGLNIQIVAGRTYDVLACCDLALVTS
                     GTATLETGLMGVPMVIVYKVSLFSELIGRMIIRQQNIGLVNILAGKTVAPELIQRDAR
                     GPRIASEALAILQNREKRQQIIRELGNIRATLGEPGAAGRAAQIACNMI"
     gene            complement(1952..2182)
                     /locus_tag="PLQ77_08010"
     CDS             complement(1952..2182)
                     /locus_tag="PLQ77_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPL10661.1"
                     /translation="MIPMVDLKRQYARLKKEIDPKITPKTKAIIPVHRFGHPADMAPV
                     MDIAREVLSLPMFPELTGEEIRQAAEAIRHVC"
     gene            complement(2179..>2440)
                     /locus_tag="PLQ77_08015"
     CDS             complement(2179..>2440)
                     /locus_tag="PLQ77_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPL10662.1"
                     /translation="GYHSVDCRKREVLSFSKITGDDGKPQIVQNALELGGHDPLEEEI
                     RSFVEAVRGRSEPRVSGEDGRKSLALAIEILQKMKTGEGLRL"
BASE COUNT          514 a          675 c          694 g          557 t
ORIGIN      
        1 cggcggcgat gcccaggccg cccagaaaat ccatcagcgg gctggaaatg gcgtttacgg
       61 aaacggcctt catgctgtat ttgaacagac gctcgttttc cgcggcgaac cgatcgtttt
      121 catatttttc catgcagaag gcttttacga tgcgcgtgcc ggaaatggtt tcctgcagga
      181 gcgagttcag cgttcccatg gtaatctgcg aggaggtggt cacctttctc atcttttttc
      241 cgaaacggga aatcggataa atcgtcagcg gaaaaacaac catggcgatg agcgcgagct
      301 tccagtcggt ataaaaaaca actcccacca ggcagatcag catgaaggtg tccttgacca
      361 gcgccgtgat ggcctccgac gacgcggttt gaacggactg cacgtcgttg gtaatccgcg
      421 acatcagcaa tccggttgga tgttcggcaa aaaaggataa agactgcttc tgaatctgct
      481 catagagctg attgcgcaga ttggtgacga tgcgcaaacc gatggagctc atcagaatat
      541 tctgtccgta gttggccagc cccttgaaca gaaaaatcgc gacgacggcc aagggaatcc
      601 acttcagggc ggcgatgttt ttgctcatga aaatttcgtc gatggcgggc ttggcaatca
      661 gcggcagagc ggattgcagc cctccggcga caatcatgca gatggcggcc aggataaaac
      721 gggcgtaata ctgccgggcc attgtaaata atctttgaaa tacttccatg gttcgtccta
      781 tatcatattg caggcgattt gcgcagctct tcctgcggcg ccgggttcgc cgagtgtggc
      841 gcgaatgttg cccagctccc gaatgatctg ctgccgtttt tcccggtttt gtaaaatcgc
      901 cagagcctcc gacgcgatgc gcggaccgcg ggcgtctcgc tgaatcaatt ccggtgcgac
      961 cgttttcccg gccagaatgt tgaccagtcc gatgttctgc tgccgaatga tcattcggcc
     1021 gatgagctcc gaaaagaggg agaccttgta aacgatgacc atcggaacgc ccatcaggcc
     1081 tgtctccagc gttgccgttc cggaggtcac gagcgccaga tcgcagcagg ccagaacgtc
     1141 ataggtgcgc ccggcgacga tttgaatatt caggccggag ccttgcaaaa agccggcgac
     1201 ggttttttgc tccagggttt ccgccagggg caggatgtac tgcgccgtgg gaaatttttg
     1261 agaaatgatt tgcgccgcct gaatcatttc cggcagcagc ttctgaattt ccgaattccg
     1321 gcttccggga agcaggccga tggtcgtttt gtttgccgaa aggccgaagt gagcgcgggc
     1381 ctcctgagcc gtgaaggttg ttttcacgat gtcgagcagg ggatgcccga cccaatggac
     1441 gtcgaatccg tgcgcggcgt aaaaatccac ttcaaacggc agaatcaccg ccatcttgtc
     1501 caccagtttc ttgatctgtt caacgcgccc ttcacgccag gcccacacct gcgggctgat
     1561 gtagtaaaag acccgaatgc ctcttttttt ggcggcgcgg gccgcgatgt tcagattgaa
     1621 atcgggaaaa tcgatcagga tgaccaggtt gggcttcagc cggtccaggg atttcttcat
     1681 tcgggaaatg gtcctgagca ggacggcggt ttttgaaaac acttccgaca cgccgaccac
     1741 cgcaatatcc gccgcgtgcg ccagcagctt cacgcccgct tcccgcattt gattgccgcc
     1801 gatgccgaaa aattccacag agggattgac ggccagcatt tcccggacga gcagggcgcc
     1861 atgcagatcg ccggaggcct ctccggcaac gatcatcact ctgggagcgc ttccgctgtt
     1921 tttgttcatg atggccttcc tcccgcccgg ttcagcaaac atgacggatc gcttcggcgg
     1981 cctgccggat ttcttccccg gtcagttccg gaaacatcgg cagggaaaga acctcgcgcg
     2041 cgatgtccat caccggggcc atgtcggcgg gatggccgaa ccggtggacg ggaatgatcg
     2101 cttttgtttt tggagtgatt ttcgggtcga tttctttttt cagacgggca tactgtcttt
     2161 tcaaatccac catcgggatc ataggcgcag gccctcgccg gttttcattt tctgaagaat
     2221 ctcgatggcc agcgccagag acttgcggcc gtcctctccg gacacgcgcg gctcagagcg
     2281 gccccgcacg gcttcgacaa acgagcggat ttcttcttcg agggggtcat ggccgcccag
     2341 ctcgagagcg ttttgaacaa tttgcggttt gccgtcgtcg cccgtgattt tggaaaacga
     2401 cagaacctct cttttccggc agtccacgga gtgatagcct
//
LOCUS       DAOPAF010000122         2389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_34903_length_2389_cov_42.380034, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000122 DAOPAF010000000
VERSION     DAOPAF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34903_length_2389_cov_42.380034"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLQ77_08020"
     CDS             <1..925
                     /locus_tag="PLQ77_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPL10663.1"
                     /translation="VEVNDCADWAVFAKNGADAISWAVALARGYRKRDKIVLAHGAYH
                     GTQPWAGTNHIGVLPADRADVLMAPWGDANAFRRVVEENKNRIAGALFTPYHHPVFEP
                     QALPAPGFWRDMRRICDENDIVLIIDDVRAGWRLDIKGSAHYFGFRPDLACYCKAIAN
                     GYPISALVGTDRMRMVAAKAFQTGSYWNNSPEMAAALACIDEMQKIDAAAICDSRGGE
                     LRRGLEERAAAHGLSFRMSGPNAIPFLHFTNESNFKRAQLFCRECSLRGVYFFWHHNM
                     FLSAAHTEADIQKTIDVADQAFQIVKDQFGE"
     gene            complement(1044..1499)
                     /locus_tag="PLQ77_08025"
     CDS             complement(1044..1499)
                     /locus_tag="PLQ77_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPL10664.1"
                     /translation="MTRILIVYHSQTGHTKQMADAVCEGAKAIDDVEVVFKKASEATL
                     DDLLACDGLAVGTPENFGYMSGMLKDFFDRTYVGAQDRVFRKPFVVFISAGNDGSGAL
                     RAIERIALGYKFKTVFAPVIARGNITEEVLDQCRELGGTLAGGCAMGIY"
     gene            complement(1632..>2389)
                     /gene="tpiA"
                     /locus_tag="PLQ77_08030"
     CDS             complement(1632..>2389)
                     /gene="tpiA"
                     /locus_tag="PLQ77_08030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPL10665.1"
                     /translation="RILMRKWIVAGNWKMHNTIAESIALARAIKEGFSGSANGEVVVA
                     PPFTALSAVGDALKGSAVRLAAQNMYSEDKGAFTGEISPVMLKDAGCACVIIGHSERR
                     KFFSETDDGVNAKVKKALAAGLKPIVCVGETDEERVQGVTQSVVGRQVRGALAGVEKL
                     DDVVIAYEPVWAIGTGKVATSAQAEEVHAFVRGLIRELFGAAAEELRILYGGSVTKDN
                     VAELAAMEDIDGALVGGASLKPDGFLGIIAKMP"
BASE COUNT          460 a          743 c          685 g          501 t
ORIGIN      
        1 ggtggaggtc aatgactgcg ccgactgggc cgtgtttgcc aaaaacggcg cggatgcgat
       61 ttcctgggcc gtggcgcttg cgcgcggcta ccgaaagcgc gacaagatcg tgctggccca
      121 cggggcgtat cacggaaccc agccgtgggc gggcaccaac cacatcggcg ttcttccggc
      181 cgaccgggcg gacgtcctca tggcgccgtg gggcgatgcg aacgcctttc ggcgggtcgt
      241 ggaggaaaac aaaaaccgga tcgccggcgc cctgttcacc ccctatcatc atcccgtctt
      301 tgagccgcag gcgcttccgg cgcccggatt ctggcgggac atgcgccgga tttgcgatga
      361 gaatgacatc gtcctgatca tcgatgacgt ccgcgccgga tggaggctcg acatcaaagg
      421 ctccgcccat tatttcggct tccggcccga tctggcctgt tactgcaagg cgatcgccaa
      481 cggctatccc atctccgccc tggtcggcac ggaccggatg cgcatggtcg ccgcgaaagc
      541 ttttcagacc ggctcctact ggaacaattc tccggaaatg gcggcggccc tggcctgcat
      601 cgacgaaatg caaaaaatcg acgccgccgc gatctgcgac tcccgcggcg gcgagcttcg
      661 ccggggactg gaggaacgag ccgcggcgca cggcctttcg tttcgaatgt ccggaccgaa
      721 cgccatcccc tttctgcatt ttaccaatga aagcaacttc aagcgcgccc agctcttctg
      781 cagggaatgt tcactgcgag gcgtttactt cttctggcat cacaacatgt ttttgagcgc
      841 ggctcatacc gaagcggata ttcagaaaac cattgatgtg gccgaccagg cctttcagat
      901 cgtcaaagat caattcggag aataaccgtc agaatccatc gtgtgatgat gttttgggca
      961 gcgacaaaac ccgccttcgg ctcaaaccgt tgtcatgact gatctttcgc ctatggccta
     1021 ttgcccgtga cccattgcct catttaatag atgcccatcg cgcagccgcc ggccagcgtg
     1081 cctcccagct cgcggcactg gtcgagcact tcttcggtga tgttcccgcg ggcgataacg
     1141 ggcgcgaaga cggtcttgaa tttatagccc agagcgatgc gttcgatggc cctgagcgcg
     1201 cccgagccgt cgttcccggc gctgatgaag acgacaaagg gtttgcggaa aaccctgtcc
     1261 tgcgcgccga catacgtgcg gtcgaagaaa tccttgagca tgcccgacat gtagccgaag
     1321 ttttccggcg tgccgacggc caggccgtcg caggccagaa ggtcgtctag cgtggcctcg
     1381 gaggcttttt tgaaaaccac ctcgacgtcg tcaatggcct tcgctccttc gcaaacggcg
     1441 tctgccattt gtttggtgtg tcccgtctgg gaatggtaaa cgattaagat tctggtcatg
     1501 ataaaaggca atgggttatg ggtgataggt gataggttat gggtgatggg ttaggggaga
     1561 aaagcggcga aaggacttct gtttcccggt cctctcgcct cttacctctc gcccattacc
     1621 cataacctct tttacggcat tttggcgatg atgcccagaa agccgtccgg tttgagcgat
     1681 gcgccgccga cgagcgctcc gtcgatgtct tccatagcgg ccagttccgc gacattgtcc
     1741 ttcgtcacgc tgccgccgta aagaatgcgg agctcttcgg ccgccgcccc gaacaattcc
     1801 cgaatcagac cgcggacgaa agcgtgcacc tcctcggcct gcgcggaagt ggcgactttg
     1861 cccgtgccga tagcccagac cggctcgtag gcgatcacca catcgtccag cttttcaaca
     1921 ccggccagag cgccccgcac ctggcggccg acaaccgact gcgtcacgcc ctgaactctt
     1981 tcctcatccg tttcacccac gcagacgatc ggcttgagcc ccgcggcgag cgcctttttc
     2041 actttggcgt tgacgccgtc atccgtttcg gaaaaaaact tgcgccgttc cgaatggccg
     2101 atgatgacgc aagcgcagcc ggcatccttg agcatgacgg gggaaatttc gcccgtgaag
     2161 gcgcccttgt cctcgctgta catgttttgc gcggccagcc gcaccgcaga acctttaagt
     2221 gcgtcgccca cagcggaaag ggcggtaaac ggcggcgcca ccacgacttc gccgtttgcc
     2281 gagccggaaa atccttcctt aatcgcgcgg gccagggcga tcgattcagc gattgtgttg
     2341 tgcatcttcc agttgcctgc gacaatccat tttctcatga ggatcctcc
//
LOCUS       DAOPAF010000123         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_36821_length_2296_cov_48.456939, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000123 DAOPAF010000000
VERSION     DAOPAF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36821_length_2296_cov_48.456939"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..752)
                     /locus_tag="PLQ77_08035"
     CDS             complement(24..752)
                     /locus_tag="PLQ77_08035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPL10666.1"
                     /translation="MEIVALSQISPDQVVVWSWNFITLNVTILYTWLVMGILVGGSIW
                     VTRNLSSEMNVSRWQHFLEIVVSIVRGEISEMTKKGADNYVPLIGTLFLFICTSNVLA
                     VVPGYIAPTSSMTTTAALATCVFVAVPYYGISRNGVLHYVKEYFQPNFIFFPFHVMGE
                     LSRTLALTVRLFGNIMSHEKVIGILLAVTPFLFPVVMQILGLLIGVIQAYIFAILSMV
                     YIASALSAEDEDHHAEAKKEGSAA"
     gene            complement(789..1091)
                     /locus_tag="PLQ77_08040"
     CDS             complement(789..1091)
                     /locus_tag="PLQ77_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPL10667.1"
                     /translation="MTPNALGMFVLMGAAGMALGALYFVGLWQTVKRLPQTEGRGLLL
                     LVSLVARLSVLLAIFYVLLQDGRWERLAAVLIGFVVMRKILTVRLGPQKTASAMQE"
     gene            complement(1093..1422)
                     /locus_tag="PLQ77_08045"
     CDS             complement(1093..1422)
                     /locus_tag="PLQ77_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPL10668.1"
                     /translation="MVEIRRTTNRRRQMADHFAEKVAQREALRIKGLKHKNETVWFGL
                     GMFGIVGWAVAIPTLIGVALGLWIDRTWPSQYSWALMGLVAGVIVGCINAAYWVRKIR
                     SRIIEEE"
     gene            complement(1480..1860)
                     /locus_tag="PLQ77_08050"
     CDS             complement(1480..1860)
                     /locus_tag="PLQ77_08050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPL10669.1"
                     /translation="MLLKILLPAEVMMEREVKKIVAEAENGSFCLMPNHIDFVATLAP
                     GIFSFVPTEGGQELLAMDVGTLVKKGSDVLVSTRNAVKAAELGKLKQVVVEQYDALDE
                     REKLVRSAAAKLEASLIRRFVELK"
     gene            complement(1850..>2296)
                     /locus_tag="PLQ77_08055"
     CDS             complement(1850..>2296)
                     /locus_tag="PLQ77_08055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPL10670.1"
                     /translation="TFGHLSATIALSRKRASEGLYPAIDLLQSGSKMLMPNIAGERHY
                     KIAQEIRKTLAVYEDLKDIIAMLGISELSREDQRTVFRARRLERFFTQPFFVTEQFTG
                     TAGKMVSREETLNGCERILNDEFAEYPERALYMIGPIEEAKIEHVA"
BASE COUNT          517 a          694 c          603 g          482 t
ORIGIN      
        1 actcctcctt tatttcaatt ttatcaggct gcagagccct cttttttggc ttccgcgtga
       61 tgatcttcgt cctcggcgga aagcgccgag gcaatatata ccatggaaag aatggcgaaa
      121 atgtaggcct gaatcacgcc gatcagaagc cccagaatct gcatcacgac gggaaacaga
      181 aagggcgtga cggccagcag aattccgatg accttttcat gactcatgat gttgccgaaa
      241 agacgaacgg tgagcgcgag cgtccgggac agttcaccca tgacgtggaa cgggaagaaa
      301 ataaagttcg gctgaaaata ctcctttaca taatgcaaaa cgccgtttcg ggaaattcca
      361 tagtacggaa cggcgacaaa tacgcaggtg gccagcgccg ccgtggtcgt catggaagac
      421 gtcggcgcga tatatcccgg caccacggcc agcacattgg aggtgcagat gaataaaaag
      481 agcgtgccga tcaggggaac ataattgtcc gcccccttct tcgtcatctc gctgatttct
      541 ccgcggacga tggaaaccac gatctccagg aaatgctgcc agcgcgatac attcatttct
      601 gaagaaagat tgcgggtcac ccaaatggaa ccgcccacca gaatgcccat caccagccag
      661 gtatagagaa tggtcacatt gagcgtgatg aaattccagc tccagacaac cacctgatcc
      721 gggctgatct gacttaacgc gacgatttcc atagaatctc cttccccggt tgatcaaaac
      781 ccttttcgtc attcctgcat cgctgacgcc gtcttttgcg gacccagacg aaccgtcagg
      841 attttgcgca tcaccacgaa accgatcagg accgcggcca gtctttccca gcggccgtcc
      901 tgaagcaaaa cgtagaatat ggccaaaagg acggacaggc gcgccaccag gctgaccagc
      961 agaagaaggc cgcggccttc cgtttgcggc agcctcttca cggtctgcca cagaccgaca
     1021 aaatagagag cgcccagcgc cattcccgcg gcacccatca aaacaaacat ccccaaagcg
     1081 ttcggcgtca tgtcattcct cttcgatgat ccggctgcgg atttttctga cccaataggc
     1141 cgcgttgatg cagccgacga tcacgccggc caccagcccc atgagcgccc aggaatactg
     1201 gctgggccag gtccggtcga tccagagccc cagggccacg ccgatcagcg tgggaatcgc
     1261 caccgcccat ccgacgatgc cgaacattcc caggccgaac cagaccgttt cattcttatg
     1321 cttgaggcct ttgatgcgca gcgcctcccg ctgggcgact ttttccgcga agtgatccgc
     1381 catctgccgg cgccggttgg tggttcttcg tatttcaacc atgttgagat tccttccttg
     1441 ctcgtttggg gtccgtgcgg cgcgttcggg gatcgcgccc tacttcagtt ccacgaagcg
     1501 ccgaatcagc gacgcctcca gttttgccgc ggcggagcgg accagttttt cccgctcgtc
     1561 gagcgcgtca tactgttcca ccaccacctg cttgagcttt cccagttccg ccgccttcac
     1621 ggcattgcgc gtggacacca gaacatccga tccctttttc accagcgtgc cgacatccat
     1681 ggccagaagt tcttggccgc cttcggtcgg cacaaacgaa aagatgcccg gggccagcgt
     1741 ggcgacaaaa tcgatatggt tcggcatcag gcagaaagaa ccgttttccg cttccgcgac
     1801 gattttcttg acctcccgct ccatcatgac ttccgccggc aataaaattt taagcaacat
     1861 gttctatctt cgcctcttca atcggtccga tcatgtaaag cgcgcgctcc ggatactcgg
     1921 caaactcatc gttcaggatc cgctcgcacc cgttgagcgt ttcttcccgg gaaaccattt
     1981 ttccggccgt gccggtgaac tgctcggtga caaagaaagg ctgcgtgaaa aaccgctcca
     2041 gccggcgcgc gcggaacacg gtgcgctgat cctcgcggga cagttcggaa atgcccagca
     2101 tggcgataat gtccttgagg tcttcataca ccgcgagggt cttgcgaatt tcctgggcga
     2161 ttttgtagtg ccgttcaccg gcgatgttgg gcatcagcat tttggatccc gactggagca
     2221 gatcgatggc gggatacaag ccttcactgg cgcgcttgcg cgaaagcgcg atggtcgcgg
     2281 acaggtgacc gaaggt
//
LOCUS       DAOPAF010000124         2295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_36847_length_2295_cov_40.341518, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000124 DAOPAF010000000
VERSION     DAOPAF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36847_length_2295_cov_40.341518"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLQ77_08060"
     CDS             <1..476
                     /locus_tag="PLQ77_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL10671.1"
                     /translation="ILGAPLGGFLADFWQRKNPRGRMYIPVVAYILGGGLLIVVVLTR
                     FSYVGIALACVYGIAAAAAMPAIAAISQDVVPVAHKGLSMGLAIFAQYMLGGAWGPYI
                     VGAVSDGLGGGADGLSIAVMLCGVFGIVAGILFLVASRTYPEDLQKVKDEAILEE"
     gene            704..2029
                     /locus_tag="PLQ77_08065"
     CDS             704..2029
                     /locus_tag="PLQ77_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPL10672.1"
                     /translation="MDERKIMEDAVQTMSRYVRFDTTNPPGNEMPAALWVADQLTARG
                     ITSDIRIHEPVAGRGLVVARIAGTEAAKPLMLNHHIDVVAADASRWTHPPFSGEVTGG
                     FVWGRGTLDTKGLGVMFLLALESLLKEGVKFRRPIVFTAVPDEEPGGDNGMRWLVEHH
                     LKDIDPEWVWDEGTGGLKDVIGQGTMFAVAVAEKQIYRFRLIAAGDPGHGSMPHRNSA
                     NVTLLSALSRIVGNPRPLRVDKAAEMMFRGLAPTQKFPASLLLRHLSFPPALMLAGRA
                     LAADKFTNAVLRDTVSPNVIQGGYQINVIPEQAAAELDCRLLPSTRAEEFHRWLVSRI
                     ADDRIRIEMIQTSPPSGIAPLDSPFYRALQAGVQKHCPGAGVFPLLMAGATDGRYWRE
                     RGYPAYGFTPMLLERADLGRVHGIDERISAENLLFGIRMVRDILRTLCL"
     gene            complement(2084..>2295)
                     /locus_tag="PLQ77_08070"
     CDS             complement(2084..>2295)
                     /locus_tag="PLQ77_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL10673.1"
                     /translation="GLFFASFMQFLTISTVNFQTFSELAFRFTLTPGIIIESMIFALL
                     MGFLGGMLPALRASRLRIVDALRAA"
BASE COUNT          432 a          670 c          753 g          440 t
ORIGIN      
        1 cgattctcgg cgcgccgctg gggggatttc tcgccgattt ctggcagagg aaaaaccccc
       61 ggggccgaat gtatattccg gtcgtggctt atattctggg cggcgggctc ctgattgtgg
      121 tggtgctcac gcgattcagt tacgtaggca tcgcgctggc ctgcgtgtac ggcattgcag
      181 cggcggcggc catgccggcg attgccgcca tctcgcagga cgtcgtcccg gtggcgcaca
      241 agggcctgtc gatggggctg gcgatcttcg cgcagtacat gctgggcggc gcctgggggc
      301 cttacatcgt cggcgcggtt tccgacgggc tgggcggagg cgcggacggt ttgagcatag
      361 ccgtgatgct ttgcggcgtc ttcggcatcg tggcgggcat tctctttttg gtcgcctcgc
      421 gcacgtatcc ggaggatttg cagaaagtaa aggacgaggc gattctggaa gagtagtgaa
      481 tggttgtagg gaacggtcgc gaccgttccc tacaggttat gggccagagg tcatgggcca
      541 tggggaaaag gtggcgcgag actgaaggct gaagaatgta gggcgggcct tcaggcccgc
      601 catggcggat ggtggccggt tgcagggaac ggtcgcgacc gttccctgcg ggcaatgggt
      661 tacggacgag aggaaaaggg cgataggcta taggggaaaa tcgatggatg agagaaaaat
      721 aatggaagac gcggttcaga cgatgagccg ctacgtgcgg ttcgacacga ccaatccgcc
      781 gggaaatgaa atgcccgccg ctctttgggt cgccgatcaa ctgacggccc ggggcatcac
      841 ttccgacatt cgaattcatg aaccggtcgc ggggcgcggc ctggtcgtgg cccgcatcgc
      901 gggaaccgaa gccgcaaagc cgctgatgct caaccaccac atcgacgtcg tcgcggccga
      961 cgcttcccgg tggacccacc cgcccttttc cggagaggta acgggcggct ttgtctgggg
     1021 gcgcggaacg ctggatacca agggcctcgg cgttatgttt ctcctggcgc tggaatccct
     1081 gctcaaggaa ggcgtgaaat tccgccgtcc cattgtgttt acggccgtgc ctgacgaaga
     1141 gccgggcgga gacaacggca tgcgctggct ggtggagcat cacctgaaag acatcgaccc
     1201 cgaatgggtg tgggatgaag gcaccggagg cctgaaagac gtcatcggac aaggcaccat
     1261 gtttgccgtc gccgttgccg aaaagcagat ttaccgcttt cgtctcattg ccgcgggcga
     1321 ccccggacac ggctccatgc cgcaccggaa cagcgccaac gtgacgctgc tttccgccct
     1381 ttcgcggatc gtcgggaatc cccggcctct gcgcgtggac aaggcggccg aaatgatgtt
     1441 tcggggactg gccccgacgc agaagtttcc cgcgtcactg ctgcttcgcc atctgtcttt
     1501 cccgccggcc ctgatgctgg ccggccgtgc gctggccgcg gacaaattca ccaacgccgt
     1561 tttgcgcgac accgtcagcc ccaatgtcat tcagggcggc taccagatca atgtcatacc
     1621 ggagcaggcc gcagccgagc tggattgccg tctgcttcct tccacccgcg cggaagaatt
     1681 tcatcgctgg ctggtctccc gcatcgcgga tgaccggatt cgaatcgaga tgatccagac
     1741 gtcgcctcct tccggaatag cgcccttaga cagtccgttt taccgggcgc tacaggccgg
     1801 cgtgcaaaag cactgtcccg gggcgggcgt gtttccgctc ttaatggccg gagcgacgga
     1861 cggccgctac tggcgggaga ggggctaccc ggcttacggg tttacgccga tgctcctgga
     1921 gcgcgcggat ctcggacgcg tgcacggcat cgacgagcgc atctccgcgg agaatcttct
     1981 tttcggcatc cggatggtcc gggacatcct ccgcacgttg tgtttgtagg gaacggtcgc
     2041 gaccgttccc tacaaccatc caccattcac tattcaccat ttactacgcg gcgcgcaggg
     2101 cgtccacgat tctcaggcgc gaggctctga gcgccggcag catgccgccc aggaatccca
     2161 tgagcagcgc gaagatcatc gattcgatga tgatgccggg cgtcagcgtg aaccgaaacg
     2221 caagttcgga aaaagtctgg aagttcaccg tggaaatcgt cagaaactgc atgaacgagg
     2281 cgaaaaacaa tccga
//
LOCUS       DAOPAF010000125         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_37004_length_2288_cov_35.730408, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000125 DAOPAF010000000
VERSION     DAOPAF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37004_length_2288_cov_35.730408"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /locus_tag="PLQ77_08075"
     CDS             complement(<1..2194)
                     /locus_tag="PLQ77_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPL10674.1"
                     /translation="MTPGQAAAYQSLSPAQQQAIQGELGKTGGKVTPQAVDALKGRPE
                     FQGVSPAEIEKGKQMLEQKEKAAEKAPEKPIEKASDDAKEKGAEKIEKKNLPWEEKTI
                     IGEEAVENGLFDRSRRVGKYQDVSLSLQLFGSDFFRESAVRVVTDRKDIPVPLKYVIG
                     PGDEVKLLLWGRLNAQYNLTVDRDGKITIPQVGPMFVAGMTFEEMSKHLITQAEQIVG
                     TNIDVSMGALKTISVFVLGDVRKPGAYTIGSFATITDALVMVGGASNIGSMRRVQLRR
                     NNKLVTTFDLYDLLLKGDKSKDVILQSGDVIFVPVAGPLVGIAGNVKRPAIYELKDKH
                     DLHTVIENAGGIIPTAYTQQIQVERIIKGEKHVVVDINDKNLEHASQFQLQDADLVKI
                     FSIVDANVNAVYINGNVKRPGKYEYKPGMTIRDILSRPDELLPETHYDYALIRRLTPP
                     SMEAALVPFNLGKLIFDHDPAQNIALMPQDQIYIFSKWFFQEKPHFSISGEVRKGGRF
                     DLPDNFTVKDAILAAGDLTKEAYLKKGEIVRVNQQREYKTIYFNVANALAGDVGDNVL
                     LKDEDRIVIHSIWEERWREVVSVVGEIKNPLQTQLTESMRVSDLVFKAGGVTRDTYYN
                     RAELYRTDWRTKEVTLREIDLGKALAKDPENNLLLKDLDRLVVHSVREKIFEKTVSID
                     GDVHKPGTYRLAEKMTVRDLVFAAGNVTEAVYLDDAEIATMRVEDGKTA"
BASE COUNT          517 a          661 c          532 g          578 t
ORIGIN      
        1 tggcggtctt gccgtcttcc acccgcatgg tggcaatttc cgcatcgtcc agataaaccg
       61 cttccgtgac gtttccggcg gcaaaaacca ggtctctaac cgtcattttt tccgccagcc
      121 gatacgttcc cggcttatga acatcgccgt caatactgac cgttttttca aaaatctttt
      181 cccgaacgga atgaacgacc aggcgatcca gatcctttaa aagcaaatta ttttcaggat
      241 ctttagccag ggccttgccc agatcaattt cccgcaacgt tacttctttt gtgcgccagt
      301 cggtgcgata cagctcggcg cgattgtaat acgtgtcgcg ggtaacaccg cctgccttga
      361 aaacaagatc cgacacgcgc atcgactccg taagctgtgt ttggagagga ttcttgattt
      421 ctccaacaac ggaaacaact tccctccatc tttcctccca gatggaatgg atcacaatcc
      481 ggtcttcatc cttcagcaga acattatcgc cgacatcacc cgccagagca ttggccacgt
      541 tgaaataaat cgtcttgtat tctctctgtt gattgacgcg aacaatttct ccctttttca
      601 aataggcttc cttggtcaga tcgccggcgg ccaaaatagc gtctttgacg gtaaaattat
      661 ccggcagatc aaaacgtccg cccttgcgca cctcgccgct aatggaaaag tgaggctttt
      721 cttgaaaaaa ccatttactg aatatatata tctgatcctg gggcatcaaa gcgatattct
      781 gcgcgggatc atgatcgaaa attaattttc ccagattgaa gggcaccagc gcagcctcca
      841 tcgaaggcgg cgtcagacgc cgaatcagcg cgtagtcata atgcgtctcc ggcagcaatt
      901 catccggccg ggaaagtata tccctgatcg tcatacccgg tttgtattcg tatttgcccg
      961 gccttttcac attgccgttg atataaacgg cattgacatt agcgtcaaca atggagaaaa
     1021 ttttcaccag atctgcgtcc tgaagctgaa attgagacgc atgttccaga tttttgtcgt
     1081 taatatccac gacaacatgc ttttcaccct tgataattct ctccacctga atttgctgcg
     1141 tgtaagcagt ggggataatt ccccccgcgt tttcaatcac cgtatggaga tcatgtttgt
     1201 ctttgagctc ataaatcgcc ggccgtttca cattgccggc aatgccgacg agagggccgg
     1261 ctaccggcac aaaaatcacg tccccggact ggaggatgac gtccttggac ttgtcgcctt
     1321 tcagcaaaag atcgtagagg tcgaacgtgg ttaccagctt gttgttgcgc cgcagctgaa
     1381 cacgccgcat cgatccgata ttggacgctc cgcccaccat caccagcgcg tccgtaatcg
     1441 tagcgaaaga gccgatcgtg taagcgcccg gtttgcggac atcacccagc acaaagacgg
     1501 aaatcgtctt cagcgcgccc atggaaacgt cgatattcgt ccccacaatc tgctcggcct
     1561 gcgtgatcag atgcttggac atctcctcaa aagtcattcc ggcgacgaac atcggcccca
     1621 cctgcggaat cgtgatcttg ccgtcccgat ccaccgtcag gttgtactgg gcgttgaggc
     1681 gcccccatag caaaagtttg acctcatccc ccggcccgat tacatacttc aacggaacgg
     1741 gaatgtcctt gcggtcggtc actacacgaa ccgccgattc ccgaaagaaa tcagatccaa
     1801 acagttgcaa actaagagaa acatcctgat atttgccaac ccggcgtgaa cgatcaaaca
     1861 gaccgttttc caccgcttcc tcgccgatga tcgttttttc ttcccagggc agatttttct
     1921 tttcaatctt ttctgcgccc ttttccttag cgtcatccga agccttttca atcggttttt
     1981 ccggcgcttt ttccgcggct ttttccttct gttccagcat ctgcttgcct ttttcgattt
     2041 cggcaggaga aacaccttgg aattccggac ggcctttcaa agcatcgacc gcttgcggcg
     2101 tgaccttgcc gcccgttttt cccagttcgc cctggatggc ctgctgctgc gcaggagaaa
     2161 ggctttgata cgccgccgcc tgccctggcg tcatctgtgg aagaccggcc gccggagaag
     2221 cggactgcgc cggggcaacg gaaggctgcg gagacacaga tggcgctgga gttgcggact
     2281 gcgccggc
//
LOCUS       DAOPAF010000126         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_37657_length_2258_cov_41.737631, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000126 DAOPAF010000000
VERSION     DAOPAF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37657_length_2258_cov_41.737631"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLQ77_08080"
     CDS             complement(<1..487)
                     /locus_tag="PLQ77_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10675.1"
                     /translation="MKKSYSEKVVSAETAIRSISRGKRVFIGSYCGEPQHLVSALLDH
                     TDHFSDVEVVRFLNLEGSLMGLVAEETQGRNYHVRSIYQGSGMIRGLTASRRFLTPMN
                     LYTVPSLFQKRHIPIHYALIQTAPPDEFGWLNLGISVDITLAAAHAADVVIVQVNPNM
                     PV"
     gene            complement(498..2033)
                     /locus_tag="PLQ77_08085"
     CDS             complement(498..2033)
                     /locus_tag="PLQ77_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10676.1"
                     /translation="MTDKKQGRFLGEATAFLALFLDNAGVLIFLNAILVFTFNFPADI
                     VLTHMIPGTAIGVFMGDLIYTWMAVRLKRKTGREDVTAMPLGLDTPSTIGIAYAVLGP
                     TYIASQDAILTWHVGMATLFMIGVVKIFTSFIGGWIQRNIPTAGLLGSIGGVGLMLLG
                     FLPMIEIFNEAIVGLVALGLIFAALYSRLQFPGRIHGVVAALLVGTGIHFALGWSGCL
                     PEVQPPSWDLHLCLPVFSLDFFKILPHSLQYLPLAIPFGILTIIGGINNTESARLAGD
                     AYRPRDILLTEAVTSLISAFFGGVAQTTPYIGHPAYKKMGATWRYTLATGLLVGVGSL
                     VGLMSLAVSLVPRAVIAPIFLFIGFEIVHQAYRESPPAHSPAVSLSMLPVIASLVTII
                     LGQFLAALNIPANQLPERLQQLHQTLNLLANGFILTGLLWGSLLAFLIDHKAKLAALC
                     AAVCGVLTLFGVIHSVLPTGELYLPWQAAGGAHYQLALAYGILSVILLILTGRTDLQK
                     HSH"
BASE COUNT          549 a          614 c          681 g          414 t
ORIGIN      
        1 ccaccggcat gttcgggttg acctgaacga tgacgacatc cgccgcgtgg gcggccgcca
       61 gcgtgatgtc cacggaaatg cccaggttga gccacccgaa ttcatcggga ggcgcggtct
      121 gaataagcgc gtagtgaatg ggaatatggc gcttctgaaa caaagacggg accgtgtaga
      181 gattcatggg ggtcagaaaa cgccgggaag ccgtcagccc ccggatcatg cccgatccct
      241 gatagatgga gcgtacgtga taattgcgcc cttgtgtttc ttccgcaacc aagcccatga
      301 gagagccttc caggttgaga aagcggacca cttccacatc cgagaaatgg tcggtgtggt
      361 ccagaagcgc ggaaacgagg tgctgaggct ctccgcagta cgaaccgatg aacacccttt
      421 ttccccggga aatggaacga atcgccgttt cggcggaaac cactttttcc gaataggatt
      481 ttttcatgct gcctccttca gtgggaatgt ttttgtaaat ccgttcttcc cgtcagaatc
      541 aacaaaatga ccgacaggat tccgtaggcc agggcaagtt ggtaatgcgc tccccctgcc
      601 gcctgccagg gcagataaag ctctcccgtg ggcagaaccg aatgaatcac gccaaaaagc
      661 gtcagcacgc cgcagacggc ggcgcacagg gccgcaagct tcgctttgtg atcgatcaga
      721 aaggcaagca ggctgcccca gagaagcccg gtcagaataa agccgttcgc cagcagattc
      781 agcgtttgat gcaactgctg caaacgctcc ggcagttggt tggctggaat gttcagggcg
      841 gcaagaaact ggccgagaat gatggtcacc aggctggcga taaccggcag catgctcaga
      901 ctcaccgcgg gggaatgagc cgggggagat tcccggtagg cctgatggac gatttcaaaa
      961 ccgatgaaaa gaaagatggg cgcgatgacc gcccgcggca ccagactgac cgccagcgac
     1021 atcaacccga ccagagagcc gacgccgacg agcaaaccgg ttgcaagcgt gtagcgccag
     1081 gtcgccccca tctttttata ggcgggatgg ccgatgtagg gggttgtctg cgccacgccg
     1141 ccgaaaaagg cggaaatgag cgacgtgacg gcttcggtga gaagaatgtc ccgggggcgg
     1201 taagcgtcgc cggccagacg ggcgctttcc gtgttgttga ttcctccgat aatcgtcagg
     1261 attccaaagg gaatggccag tggaaggtac tgaaggctgt gcggcaggat cttgaaaaaa
     1321 tcaagggaaa ataccggaag acacaggtgc agatcccagg agggcggctg aacttcgggc
     1381 aggcagccgc tccaccccag cgcaaagtga attcccgtgc cgacgagcag ggccgccacg
     1441 acgccgtgaa tgcgtccggg aaattgaagc cggctgtaga gcgcggcaaa gatcagcccc
     1501 agggcgacca gaccgacgat cgcttcgtta aagatctcga tcatcggcag aaagcccaaa
     1561 agcatcagtc cgacgccgcc gatggacccg agcagaccgg cggtgggaat attgcgttga
     1621 atccatccgc cgataaaaga ggtaaaaatt ttcaccacac cgatcatgaa cagcgtggcc
     1681 atgccgacat gccaggtgag gatggcgtcc tggctggcga tataggtggg ccccaggacg
     1741 gcataggcga tgccgatggt ggacggcgta tcgaggccca gcggcattgc cgtaacatcc
     1801 tcgcggccgg ttttccgctt cagacggaca gccatccagg tgtagatgag gtcgcccata
     1861 aaaacgccga tggccgtgcc ggggatcatg tgcgtcagga caatgtccgc cggaaaattg
     1921 aaggtaaaga cgagaatggc gtttaaaaaa atcagcacgc cggcgttgtc cagaaacagg
     1981 gcgagaaaag ccgtcgcttc acccaagaat ctgccttgtt ttttgtccgt catcgccagg
     2041 gttccttccg ctctttgcca aaaaacaggc tctttcgtcc gaacaccgtg ggtcagctat
     2101 agccgaaacc ggacggtttg acaattgcct tttcgccgat cggttgcctc ttggatcaat
     2161 gctgccaaag gaagagcagg tgaggcacaa agtccgcttg acacgcgggc ggcacttccg
     2221 gtaattttct accgtaatgt tctttgtatc ccgtaagc
//
LOCUS       DAOPAF010000127         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_38498_length_2221_cov_42.817175, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000127 DAOPAF010000000
VERSION     DAOPAF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38498_length_2221_cov_42.817175"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ77_08090"
     CDS             <1..251
                     /locus_tag="PLQ77_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPL10677.1"
                     /translation="GEWKEKDTEEQFIYKTRKKGFGPFKLELWHLPADIRGAICAELE
                     RQFAFLFDKLKVNGTRDILDRCIQAVDVPLKMPVSLKC"
     gene            402..1430
                     /locus_tag="PLQ77_08095"
     CDS             402..1430
                     /locus_tag="PLQ77_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPL10678.1"
                     /translation="MKAAVLERITNIRDHPRPLSFMEIPEPEPGEGQILVKVSVCGVC
                     HTELDEIEGRTPPSSFPFILGHQIVGIVEKRGARATKFSEGRRVGIGWIGSACGTCQY
                     CRGGMENLCPDFKATGRDLPGGYAQYVAVLEDFAFPVPDTFSDAQAAPLLCAGAIGWR
                     SLRLAGLSDGEPLGLTGFGASAHLVLQLARFLYPHSKISVFARSEHERRFALALGAGW
                     VGDTSEKSPEKLAAVIDTTPAWKPVACALENLAPGGRLLINAIRKEEADKDQLLKLDY
                     PLHLWQEKEIKSVANVARRDIAEFLDLAAKIPILPDVEEYAFFDANRALAELKAGQIR
                     GAKVLKIE"
     gene            complement(1910..>2221)
                     /locus_tag="PLQ77_08100"
     CDS             complement(1910..>2221)
                     /locus_tag="PLQ77_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPL10679.1"
                     /translation="IGTSRSVWAHPPRIEKPPFYALRFMPLTRKSMGGVAIDQSCRVI
                     NVSGQTIPGLYAVGELTGLAGVNGKCTLEGTFLGASILTGRVAGRAAAAELAKRKETP
                     H"
BASE COUNT          491 a          605 c          636 g          489 t
ORIGIN      
        1 aaggcgagtg gaaggaaaag gacacggaag agcagttcat ctacaaaacc cgcaaaaagg
       61 gcttcgggcc gttcaaactg gagctgtggc acctgccggc ggatattcgc ggggccatct
      121 gcgccgagtt ggagcgccag ttcgcgtttt tgtttgacaa gctcaaagtc aacggcacgc
      181 gcgacattct ggaccggtgt atccaggcgg ttgatgtgcc gctgaaaatg cccgtgtcac
      241 tcaaatgcta gaagaagcaa tatctgcttt ttgcccgcgg tatcgatggc agggaacggt
      301 ttccacgacc tgaaggtcgc gcgatcgttc cctgctgtgt tgcgcggcgg aaatcggacg
      361 tgtccggcag tgaaacaggg tatgccggat ggagtaaagc catgaaagcg gccgtactgg
      421 agagaataac gaatattcgg gatcatccgc ggccgctttc ttttatggag attcccgaac
      481 cagaaccggg cgaagggcag attctggtga aagtttccgt ctgcggtgtc tgccacaccg
      541 aactcgatga aatagaaggc cggacgcctc cgtcttcctt tcccttcatt ctgggccacc
      601 agatcgtcgg catcgttgaa aaacgcggcg cgcgggcgac gaaattttcc gaaggccgga
      661 gggtcggcat cggctggatc ggttcggcct gcgggacatg ccaatattgc cgcggcggca
      721 tggaaaacct ctgcccggat tttaaggcga ccggccgcga ccttccgggc ggctacgcgc
      781 aatacgtggc ggtcttggag gattttgcct tccctgttcc ggatactttt tccgacgcgc
      841 aggccgcgcc gcttttgtgc gcgggcgcca tcggatggcg gtcgcttcgt ctggccggcc
      901 tttccgacgg cgagcccctg gggctcacgg gattcggcgc ttcggcccac ctggtcctgc
      961 agcttgcccg ctttctctat ccccattcca aaatcagtgt ctttgcccgc tccgaacacg
     1021 aacgtcgttt tgccctggcg ctgggcgcgg gctgggtggg ggacacctct gaaaaaagtc
     1081 cggagaagct tgccgccgtc atcgacacca cgcccgcctg gaagccggtg gcctgcgcgc
     1141 tggaaaatct tgcgccgggt ggaaggctgc tgatcaatgc cattcggaag gaggaggcgg
     1201 acaaggacca gttgctcaaa cttgactacc cgctgcatct ctggcaggag aaggaaatca
     1261 aaagcgtggc caacgtggct cgccgggata tcgcggaatt cctcgatctt gccgcgaaaa
     1321 taccgatcct ccccgacgtg gaggaatatg cttttttcga cgccaaccgg gcgcttgcgg
     1381 aactaaaagc cggccaaatc cgcggcgcga aggtcctgaa aatagagtag cgacgatcta
     1441 tgagcaacga gtactagaac cttatttaaa agggttcttc aggttttgtt atgcagaaat
     1501 tattttaggt tcatccggtt cttgcgtata gccgaagtca agcacccagc aattttcatg
     1561 ggcgtccaac ctttcaatcc gaaatgaatg acgatcactt ccatgggatc ccgaatattc
     1621 ctgggatgat tacattcagt catgacgtca taacgcatca cataaaaatg cgtgacggtg
     1681 aagtttgtct gaattccggc gaatgccagt tttgcgggtg aaccaaccaa acggtgaacg
     1741 gatttctcag aaggatggat gtcgcaatgt gacttcaacg cggtcattag cggcgcaccc
     1801 cgcatcttgt ggccaaaatt ttcttgcaca cacaagacgg cattttttat acttcaccgc
     1861 acaaaaagga aattcttatc aataattcaa ttcaataata attatcaatt caatgcggcg
     1921 tttccttccg ctttgccaat tccgccgctg ccgcgcgtcc cgcgacgcgg ccggtcagga
     1981 tggacgctcc caggaaagtc ccttccagtg tgcattttcc gttgacgccg gcaagacccg
     2041 tcaattcacc cacggcgtac aatccgggga tggtctgacc cgagacattg atgacacggc
     2101 aggactgatc aatggcgacg ccgcccatgc ttttgcgcgt cagcggcatg aagcgcaggg
     2161 cgtaaaatgg aggcttttcg attctggggg gatgcgccca gactgatcgt gacgtgccga
     2221 t
//
LOCUS       DAOPAF010000128         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_40930_length_2124_cov_62.466892, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000128 DAOPAF010000000
VERSION     DAOPAF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40930_length_2124_cov_62.466892"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLQ77_08105"
     CDS             <1..707
                     /locus_tag="PLQ77_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPL10680.1"
                     /translation="IGVDGALYSAMEFTGETIRALPMADRLSMANMAIEAGAKNGIFP
                     PDEITREYVKPRAKRPYAFYTSDPDAVYAQTIEIDASRLEPQVAFPHLPSNVRAISQA
                     GEVKIDQSLIGSCTNGRIEDLRVAAGILKGRKAAPHVRLIVVPATPFIYRQALQEGLL
                     EIFMDAGAVISPPSCGACLGGHLGILAEGEKSVATTNRNFVGRMGHPRSEVYLAGPAV
                     AAASAVLGRIASPEEL"
     gene            727..1212
                     /locus_tag="PLQ77_08110"
     CDS             727..1212
                     /locus_tag="PLQ77_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPL10681.1"
                     /translation="MILKGKIWKFGDNIDTDAIIPARYLNTSDPAELARHCMEDADPA
                     FMTRMTPGDIIVGGENFGCGSSREHAPIAIKAAGISCVVAKSFARIFYRNAFNMGLPI
                     FESRDLADAIAEGAKITLDSAQGSITVEGQNQTFSISPIPPFMEQLIADGGLMKHIAG
                     K"
     gene            1220..>2124
                     /gene="leuB"
                     /locus_tag="PLQ77_08115"
     CDS             1220..>2124
                     /gene="leuB"
                     /locus_tag="PLQ77_08115"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPL10682.1"
                     /translation="MTKQNLKIAVFAGDGIGPEIIREALKVLKLVADRRGISLSLASG
                     LIGGSAYDACGTPFPDESLHLALQSDAVLLGAVGGPKWEGLDYSLRPERALLGLREKL
                     GLYANLRPVTVFDELADASPLKKDLVQGIDLLILRELTGDIYFGKPRGVTKDQSGRRT
                     GVNTMIYTEDEIRRIALRAFELARGRRKKLLSVDKANVLETTELWRQVVTETGRGYPD
                     VALSHMYVDNCAMQLIRNPSQFDVIVTSNLFGDILSDEAAMLTGSIGMLPSASLGDGK
                     ALYEPIHGSAPDIAGKNIANPLATIL"
BASE COUNT          446 a          675 c          631 g          372 t
ORIGIN      
        1 gcatcggcgt ggacggcgct ctctattccg cgatggagtt cacgggcgaa accattcgcg
       61 ctctgcccat ggccgaccgg ctgtccatgg ccaacatggc catcgaggcc ggcgcgaaaa
      121 acggcatctt tccgccggac gagattaccc gggaatacgt caagccgcgc gccaaaaggc
      181 cttacgcctt ttacacatcc gacccggacg ccgtttacgc gcagacgatt gaaatcgacg
      241 cctcccgact ggagcctcag gtcgcctttc cgcatctgcc ttccaacgtc agggccatca
      301 gccaggcggg cgaagtgaag atcgatcagt ccctgatcgg gagctgcacc aacggccgga
      361 ttgaagacct gcgcgttgcc gccgggattt tgaaaggccg caaggcagcc cctcatgtcc
      421 gcctcatcgt ggttccggcc acgccgttta tttacaggca ggcccttcag gaagggcttc
      481 tggaaatctt catggacgcg ggggcggtca tcagcccgcc gtcctgcggc gcatgcctcg
      541 gggggcatct gggcattctg gcggaagggg aaaaatccgt cgccacgacc aaccgcaatt
      601 tcgtggggcg catgggccat cccagaagcg aagtttatct ggccggcccc gccgtggccg
      661 cggcttccgc cgttctgggc cgcatcgctt caccggaaga gttataaaag caaaggggac
      721 ggcatcatga tcttgaaagg aaaaatctgg aaattcgggg acaacatcga cacagacgcg
      781 atcattcccg cgcgctatct caacacgtcg gacccggctg aactggcccg gcactgcatg
      841 gaagacgcag accccgcctt catgacccga atgacgccgg gcgacatcat cgtcggcgga
      901 gaaaacttcg gctgcggctc ttcgcgggag cacgcgccca tcgccatcaa ggcggccgga
      961 atctcctgcg tcgtggccaa aagcttcgcg cgcatcttct accgcaacgc cttcaacatg
     1021 ggcctgccga tcttcgaatc ccgcgatctg gcggacgcga ttgccgaagg cgcaaagata
     1081 acgctggaca gcgcgcaggg ctccatcacc gtggaaggcc agaaccaaac attttcgatc
     1141 agcccgattc cgcccttcat ggaacagctc atcgccgacg gcggcctgat gaagcacatt
     1201 gccggaaaat gagaaaagca tgacgaaaca aaacctgaaa atcgctgttt tcgccgggga
     1261 cggaatcggt ccggaaatta ttcgggaagc cctgaaagtc ctgaagcttg tcgccgaccg
     1321 gcgcggcatc tccctttcgc ttgccagtgg gctcatcggc ggcagcgcct atgacgcctg
     1381 cggaacgccc tttcccgatg aaagcctcca tctagccctg caaagcgacg ccgtgctgct
     1441 gggcgcggtg ggcggcccga aatgggaagg actggattac agcctccggc ccgaacgggc
     1501 gcttctgggc ctgcgcgaaa aactggggct ttacgccaac ctgcggcccg tgacggtctt
     1561 tgacgagctg gcggacgcct cgccgctcaa aaaggatctg gtgcagggca tcgaccttct
     1621 gattttgcgg gagctgaccg gcgatatcta tttcggcaag ccgcgcggcg tgaccaaaga
     1681 tcaaagcggc cggcgcaccg gcgtcaacac catgatctac acggaagacg aaatccgccg
     1741 catcgcactg cgcgcgtttg aactggcgcg cggccgaagg aaaaaactcc tgtcggtgga
     1801 caaggccaat gttctggaaa ccacggaact gtggcgtcag gtcgtcacgg agaccggccg
     1861 cggctatccc gacgtggcgc tttcgcacat gtatgtggac aactgcgcca tgcagctcat
     1921 ccgcaatcct tcgcagtttg acgtaatcgt gacctccaat ttgttcggcg acattctgag
     1981 cgacgaagcc gccatgctga ccggctccat cggcatgctg ccgtcggcca gcctgggaga
     2041 cggcaaggct ctgtacgagc cgatccacgg ctccgcgccg gacatcgcgg ggaaaaacat
     2101 cgccaacccg ctggcgacga ttct
//
LOCUS       DAOPAF010000129         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_45250_length_1972_cov_34.322900, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000129 DAOPAF010000000
VERSION     DAOPAF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45250_length_1972_cov_34.322900"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>1972
                     /locus_tag="PLQ77_08120"
     CDS             53..>1972
                     /locus_tag="PLQ77_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPL10683.1"
                     /translation="MTARVLIVDDDGNNRLLLETLLKSRGFEVLSAVNGEEGLARARS
                     HHPDLIVSDILMPVMDGYTFCRQLKSDDALKRIPFVFFTASYNTPKDEEFAMSLGAER
                     FVFKPQEPEELIAILEEVLKESRLTPRRAKKPLGEEMEFFRQHNEVLFNQLEKKLLDL
                     EQANERLRMMEEQYRLSFENITDMVLVFDGNLTITSVSPSAERILGYRPEEFVGRPID
                     DLKTILTPESFQMAVQNIGQILQGEIITSAVYDVIARDGTRKYLDISGSPLVRDGKID
                     GLVCDARDVTERVLAESARRESEELFGQFMRYFPGSAYIKDSDRRIIYLTGNTEEYFG
                     VKPQEWLGKRSEDIWPEEIALLSRKDDEAVLRGEVVQSISQRPQKDGAHTWITHRFPI
                     HRHNKPPLIGCISLDITGQKQAEENLRESEKRYRELYDFLPIPVYEMDFQSTITSVNH
                     AIYETFGGTEEDFKRGFKAWQLLTPEAVEQSVENIQRLLNGEPLGGTEYRLKRLDGTE
                     FPAIVFSRLIFEQGKPVGLRGAVVNITDRKRTEEVIRESEANFRRSMDDSPLGIRVVT
                     ADGETLYANRAILDMYGYASIEEFQGVPLKQRYTPESYAAYLARRAKRDRGERDQTEY
                     TIDIVTQNGDIRNVRA"
BASE COUNT          502 a          499 c          573 g          398 t
ORIGIN      
        1 gcaacggaaa aaacacggca actcacaaag aacaacggaa gaaaggcgga aaatgacagc
       61 tcgcgtttta attgtggacg acgacggcaa caataggctc cttttggaaa cccttctcaa
      121 aagccggggg tttgaagtcc tttccgcggt caacggcgaa gaaggcctgg ccagggcccg
      181 ttcacatcac ccggacctga ttgtttccga catcctgatg ccggtcatgg acggatatac
      241 cttctgccgg cagttgaaat cagacgacgc cctcaagcgg attccctttg tgtttttcac
      301 ggcttcatac aacacgccga aagatgaaga gtttgccatg agcctcggcg cggagcggtt
      361 tgtcttcaaa ccccaggaac cggaagaact gatcgcgatt ctggaagagg tcctgaaaga
      421 aagccgtttg actccccggc gggcgaaaaa gccgctcggc gaggaaatgg agtttttccg
      481 ccagcacaat gaagtccttt tcaatcagct ggaaaagaaa ctgcttgacc tggaacaggc
      541 caacgagcgt ctgcggatga tggaggagca gtaccggctg agctttgaaa atatcaccga
      601 catggttctg gtttttgatg ggaacctcac gattacgagc gtgtcgccta gcgcggagag
      661 gatcctgggg taccggccgg aagagttcgt cggccggccc atcgacgatt taaagaccat
      721 cctgacgccg gaatccttcc agatggcggt acaaaacatc ggccagattc tgcagggtga
      781 aatcattacc tccgccgttt atgatgtgat tgccagggat ggaacccgca aatatctgga
      841 tatcagcgga tcgccccttg tgcgcgacgg caaaattgac ggactcgttt gtgatgcccg
      901 cgatgtcacg gagcgagtgc tcgcggagag cgcccggcgg gagagtgaag agctcttcgg
      961 ccagttcatg cggtattttc cgggaagcgc ctacatcaag gattcggacc ggcgcattat
     1021 ttatttgacg gggaacactg aagaatattt cggtgtaaag ccccaggagt ggctggggaa
     1081 aaggagtgag gatatctggc ctgaagagat cgcccttttg tccaggaaag acgatgaagc
     1141 ggttctgcgg ggagaagtgg tgcaatccat ctcgcaaagg ccccagaagg acggagcgca
     1201 tacctggatc acgcaccggt ttcccattca ccggcacaac aaaccgcctc tgatcggctg
     1261 catttctctg gacatcacag ggcagaaaca agcagaggag aaccttcgcg aaagcgaaaa
     1321 acggtatcga gaattgtatg attttctgcc gattccggtc tatgaaatgg attttcaatc
     1381 cacgatcaca tctgtcaacc acgcgattta tgaaactttc gggggcacag aggaggattt
     1441 caaaagaggc tttaaggcct ggcagctctt gacgccggaa gccgttgagc aatcggtgga
     1501 aaacattcag cggctgctga atggggaacc gttaggcggc acggaatacc ggttaaagcg
     1561 gctcgacggg accgaatttc ccgccattgt cttttcccgt ttgattttcg agcagggaaa
     1621 gcccgtgggc cttcgggggg ccgtcgtcaa catcaccgac cgcaaaagga cggaggaggt
     1681 catccgggag agcgaagcga attttcgccg ttccatggac gattccccgc tgggcatccg
     1741 ggttgttacc gcggacggtg aaacccttta cgccaaccgg gcgattctgg acatgtacgg
     1801 ctacgccagc atcgaagagt ttcaaggcgt tccgttgaaa cagcgctata caccggaaag
     1861 ctatgccgcg tatctggcca gaagggcaaa aagagaccgg ggcgaaaggg accagaccga
     1921 atatacgatc gatattgtca cccagaacgg cgacattcga aatgtccggg ct
//
LOCUS       DAOPAF010000130         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_48543_length_1879_cov_46.827303, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000130 DAOPAF010000000
VERSION     DAOPAF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48543_length_1879_cov_46.827303"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLQ77_08125"
     CDS             <1..254
                     /locus_tag="PLQ77_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPL10684.1"
                     /translation="ELGSSEAIKEAVLAGLGVSVLSVHAVTRELASGMLCEIPLASCR
                     IERRFYLIYLKSFEPRAHHQTFFDFLKHFKASPNSRKEG"
     gene            259..645
                     /locus_tag="PLQ77_08130"
     CDS             259..645
                     /locus_tag="PLQ77_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10685.1"
                     /translation="MNGKRVAVATAIGLLTGCYCAASLYIPALKPAGLTSGPEAWWLA
                     MLVAGRTTQGFVIGLADGIDLHPALRGAGIGALMTLQLAMMPLFGPFKLGAVLLMAAG
                     VIYGIIEDVAATWVARRFPASRQGAA"
     gene            complement(653..982)
                     /locus_tag="PLQ77_08135"
     CDS             complement(653..982)
                     /locus_tag="PLQ77_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10686.1"
                     /translation="MDEYLHVPRGGEISFMAVTLKVTAEGCLDCGNRQLLYVEGLTTV
                     GSVCCGAVECRVIYVPGFIVSRHCKTDEASGHPVSRVEPVTNPAALDEVKKLLVKTFP
                     SSLFLFV"
     gene            complement(983..1261)
                     /locus_tag="PLQ77_08140"
     CDS             complement(983..1261)
                     /locus_tag="PLQ77_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10687.1"
                     /translation="MPAEWLTFVFSSAWIDPDEKRTAGLCAREICVLAVFGEIITVQI
                     LHVNENIDKNKDRDALILLHNAVVVPVQRIDLPQTARQGKRNNMFTGD"
     gene            1279..1587
                     /locus_tag="PLQ77_08145"
     CDS             1279..1587
                     /locus_tag="PLQ77_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10688.1"
                     /translation="MDEVITRIVAVERQCSADVEQARQECAKNIESRKQSLEDQKTRA
                     FAQIIAAEKLRLTKAMEEAKRQTEETSLAFQRDCEVPFQNQVLAQTIQEEIISILFEG
                     "
     gene            1594..>1879
                     /locus_tag="PLQ77_08150"
     CDS             1594..>1879
                     /locus_tag="PLQ77_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10689.1"
                     /translation="MRNYADVPNLHTRIHAMRSRLFSLRDYVLMIREPETLPGHIFGI
                     QDSTEAKETLFEEQIAPVFNLATAHEQYTPFFIAHLRQYEAHNAKILLAMA"
BASE COUNT          436 a          508 c          521 g          414 t
ORIGIN      
        1 gcgaactggg cagctccgag gccatcaagg aggcggttct ggcgggtctg ggcgtctccg
       61 ttttgtccgt gcacgccgtg acaagagaac tcgcttccgg aatgctttgt gagattccgc
      121 tggcatcctg ccgcatcgaa cgccggtttt atctgattta cctgaagtcg tttgaacccc
      181 gtgcgcatca tcaaacattt tttgatttcc tgaagcactt caaggcgtcc ccgaacagtc
      241 gcaaggaggg ttgaaaagat gaacggaaaa agagtggcgg tggcgaccgc catcggcctg
      301 ctcacgggat gctactgcgc cgcaagcctg tatattccgg cgctcaagcc cgcgggactg
      361 acctccgggc cggaagcgtg gtggctggcc atgctggtcg caggccgcac cacgcagggc
      421 tttgtgatcg gcctggcgga cggcattgac cttcacccgg cgctgcgcgg ggcgggaatc
      481 ggcgctctga tgaccctgca gcttgccatg atgccccttt tcgggccctt caaactgggc
      541 gcggttctgc tcatggcggc cggcgtgatt tacggaatca tcgaagatgt tgcggccacc
      601 tgggttgcgc ggcgttttcc cgcaagccgc caaggcgccg catagttttt tctcagacaa
      661 aaaggaaaag agacgaaggg aaggttttga cgagcagctt tttcacctcg tcaagtgcgg
      721 cgggattcgt aaccggctcc acccggctta caggatgtcc cgacgcttca tccgttttgc
      781 aatgccggga gacgatgaac ccgggaacat aaatgacccg gcactccacc gcgccgcagc
      841 agacgcttcc gacggtggtg agcccctcga catacaggag ttgccgattg ccgcagtcca
      901 ggcatccttc ggcagtcact ttcagcgtga cggccataaa gctgatttcc ccgccgcggg
      961 gaacgtgtaa atattcgtcc attcaatctc ccgtgaacat gttgtttctt ttaccctgcc
     1021 tggccgtttg gggcaaatca attctttgaa cggggacgac aacggcattg tgaagtaaaa
     1081 tcaaggcatc cctatcctta tttttatcta tattttcgtt gacatgtaaa atttgaaccg
     1141 ttataatttc accaaacact gcaagcacgc aaatctctcg ggcgcaaagt ccagcggtac
     1201 gtttttcatc cggatcgatc caggcagaag aaaaaacaaa cgtcaaccat tcagcaggca
     1261 aatcagtggg gtgcaggcat ggatgaggtg atcacccgga ttgttgccgt tgaaagacag
     1321 tgttccgcgg atgtcgaaca ggcccggcag gaatgcgcaa agaacattga atcccgtaaa
     1381 caaagccttg aagatcagaa gaccagggcg ttcgcgcaaa tcattgccgc ggagaagctt
     1441 cgtctgacaa aagcaatgga ggaagccaaa aggcaaaccg aggagacttc gctcgctttt
     1501 caaagagact gcgaagtacc ttttcaaaac caggttctgg cgcagaccat tcaggaagag
     1561 atcatttcca tcttgttcga agggtagaaa aagatgcgaa attatgcgga tgtgcccaat
     1621 ttgcatacgc ggattcacgc catgagaagc cgcctcttct cccttcggga ctatgtcctg
     1681 atgattcggg agccggagac gctgcccggt catatcttcg gaatccagga ttcgacggaa
     1741 gccaaggaga cgctcttcga ggagcagatt gcgccggtat ttaatctggc cacggcccat
     1801 gaacaatata cgccgttttt tattgcccat ctccgccagt atgaggcgca caacgccaag
     1861 attcttctgg cgatggcgg
//
LOCUS       DAOPAF010000131         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_50513_length_1828_cov_61.086294, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000131 DAOPAF010000000
VERSION     DAOPAF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50513_length_1828_cov_61.086294"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..264
                     /locus_tag="PLQ77_08155"
     CDS             37..264
                     /locus_tag="PLQ77_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL10690.1"
                     /translation="MKRLALFVVAAVFAFTTMAIAAEKPAAAPAAAPAAVAEPAKADV
                     KKEEKKAVKKVKKAKAKKAEKKAEEAAPAAK"
     gene            complement(365..>1828)
                     /locus_tag="PLQ77_08160"
     CDS             complement(365..>1828)
                     /locus_tag="PLQ77_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL10691.1"
                     /translation="QQKFVQIFREAGENLLNIINDILDISKVEAGHIHLEHIPFDLGK
                     LVEQTGDIMSVRAHKKELELACRVAPGVPTKLMGDPTRLRQVLINFIGNAIKFTESGE
                     ILLEVKPAHSSAVNAQHHTVDLMFSVKDTGIGIPADKIQNIFERFTQADSSTTRKYGG
                     TGLGLTISKSIVDLMGGTITVESKEGKGSVFSFTAPFAMQTEMTPVEDACALVDLTGR
                     RALVIDDNANNRLILREMLTQWGTVVSSVNSGPSGLEALREAQVSGKPYDFVILDYQM
                     PGMDGLTTAVEIRKEPKWSSLAVIMLSSGYPQEDIAAAKKIGIDRFLYKPVKRNDLQE
                     AICGELGKKEEAQPQAGSAPAAAAAQKSLNILLVEDNEDNRLLISTYLKKTPHRLHMA
                     ENGAVAVEAVKAGNFNIVFMDMQMPVMDGYTATREIRQWEKAQARTAVPIIALTAYAL
                     KEDEKKSLDAGCTGHMTKPIKKARLLEALAEFAADEA"
BASE COUNT          373 a          510 c          528 g          417 t
ORIGIN      
        1 agggcttact aaaaagatca caaggaggaa tttcagatga agcgtttagc attgtttgta
       61 gtggcggcgg tttttgcatt cacgaccatg gccatcgcgg ctgagaagcc cgcggcggcg
      121 ccggcggcag ctcccgcggc agtagcggag cccgcaaagg ctgatgtgaa gaaagaagag
      181 aagaaggccg taaagaaagt gaagaaggcg aaagcaaaga aggccgaaaa gaaggctgaa
      241 gaagcagcgc cggcagcgaa gtaagttttc cagcggaagc gacaagaaaa agggacgggt
      301 tttcacccgc cccttttttt atttccaaca aaccgtatgt tgcgtttggc aacttgcctg
      361 cggatcatgc ttcatcggcg gcaaattcgg ccagcgcctc cagcagtctc gcctttttga
      421 tcggcttggt catatgtccg gtgcatcccg cgtcgaggct tttcttttcg tcctctttca
      481 gggcgtaggc ggtcagcgca ataatgggaa cggcggtcct tgcctgcgct ttttcccact
      541 gacggatctc gcgggtggcc gtatagccgt ccatcacggg catctgcatg tccatgaaaa
      601 caatgttgaa gtttcccgcc ttcacagcct cgacggcgac ggcgccgttt tccgccatgt
      661 gaagccggtg cggcgtcttt ttcaggtagg tcgaaatcag aagccggttg tcctcgttgt
      721 cttccacgag aagaatgttc aaagattttt gcgcggcggc ggcagcgggt gcacttccgg
      781 cttgtggctg tgcctcctcc ttctttccca actctccgca gatggcttcc tgcagatcat
      841 tgcgcttgac gggcttgtaa agaaaccggt cgatgccgat ttttttcgcc gccgcaatgt
      901 cctcctgcgg atagccggag gagagcatga tgacggccag cgacgaccac ttcggctcct
      961 tgcggatttc caccgccgtc gtcagaccgt ccattccggg catctgataa tcgaggatga
     1021 caaaatcata cggtttgccg gatacctgcg cctcccggag cgcctcgagg ccggacggac
     1081 cgctgttcac ggaagagaca acggttcccc attgggtcag catctcccga agaatcaggc
     1141 gattgttggc gttgtcgtcg atcaccaggg cgcggcgccc cgtcagatcg accagcgcgc
     1201 aggcgtcttc tacgggcgtc atctcggtct gcatcgcaaa aggcgcggta aaggaaaaaa
     1261 cgctcccctt tccttctttg ctttcaacgg tgatcgttcc gcccatcaga tctacgatgg
     1321 atttggaaat ggtgagcccc agccccgttc ccccgtactt gcgcgtggtg gaagaatcgg
     1381 cctgcgtgaa gcgctcaaag atgttttgga ttttgtcggc tggaattccg atgcccgtgt
     1441 ccttcacgga aaacatcaga tccacggtat ggtgctgcgc gttgacggcg gacgaatggg
     1501 cgggcttcac ctccagcagg atttctcccg attcggtgaa cttgatggcg ttgccgatga
     1561 agttgatgag cacctggcgc aggcgcgtgg gatcgcccat cagtttggtc ggcactcccg
     1621 gcgcgacccg gcaggccagc tccaactctt ttttatgggc ccggacggac ataatgtccc
     1681 cggtctgctc caccagcttt ccaaggtcga acggaatgtg ctccagatgg atatgcccgg
     1741 cctccacctt ggaaatatcg agaatgtcgt tgataatgtt cagaaggttt tccccggcct
     1801 cccggaaaat ctgcacgaat ttctgctg
//
LOCUS       DAOPAF010000132         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_53700_length_1753_cov_43.839223, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000132 DAOPAF010000000
VERSION     DAOPAF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53700_length_1753_cov_43.839223"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLQ77_08165"
     CDS             <1..1379
                     /locus_tag="PLQ77_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPL10692.1"
                     /translation="VFGMALPAFAADMMQFLKENGFADQSKDVLTIPSAALEKRADVL
                     RRRLAALDACVKGLKEEKGEALRRMNADYRAYEEVLKAMRLSVISSRAMFLTGWMDAK
                     EKTILMEIMKEVCGGGFIVSEKKDANAPVRLKNLRLFRPFELLVKTMGMPSSNELDPT
                     PLTAITYVMMFGLMFGDLGQGIVLMLCGWLLKKFGRKKAREELAQAGGILLACGGSAA
                     VCGLLYGSVFSSEHILPALWFHPTAEIMRLFFFTIMMGVVFILVGLCLNIINSFLNAD
                     YSEAFLEKRGLAILFVYAAVVVFVLLYQKTGEPPALWALGVFIFLPLMIFSLRGLLGV
                     LFMEKPKPHSISEYIVETIMEVVEIALGLFANTISFIRVGAFALSHAGLSIVTYTLAG
                     MADPSLKSAGAIIIIVCGNIFIIGFEGLICGIQSMRLEYYEFFSKFFRGDGVVFSPFT
                     LPAKASEV"
     gene            1381..1713
                     /locus_tag="PLQ77_08170"
     CDS             1381..1713
                     /locus_tag="PLQ77_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPL10693.1"
                     /translation="MTRSRQLAFLMGAIFLCALWSLFPAMGWASEGVPAKAFTDAIGL
                     AYIGAGVAVGFACLASGYAVAKIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLII
                     AIMILNKL"
BASE COUNT          331 a          461 c          531 g          430 t
ORIGIN      
        1 ttgtgttcgg gatggccctg ccggcttttg ccgccgacat gatgcagttt ctgaaagaaa
       61 acggatttgc cgaccaatcg aaggatgtcc tgaccattcc ctccgcggcg ctggaaaaac
      121 gcgcggacgt tctgcggcgg cgtcttgccg cgctggatgc ctgcgtcaag ggcctgaaag
      181 aggaaaaagg agaggccctc cggcggatga acgccgatta ccgggcctat gaggaagtgc
      241 tcaaagccat gcgcctgtcc gtgatttcat cgcgggccat gtttctcacg gggtggatgg
      301 acgcaaagga aaagacaatc ctcatggaaa tcatgaagga ggtttgcggc ggcgggttta
      361 tcgtgtccga aaagaaggac gccaacgccc cggtccggtt gaaaaacctg cggctgttcc
      421 ggccctttga actgcttgtc aaaaccatgg gaatgccctc ctcaaacgag ctggatccca
      481 cgccgctgac ggccattacc tatgtcatga tgttcggact gatgttcggc gatcttgggc
      541 agggcatcgt cctgatgctg tgcggctggc tgctgaaaaa gttcggccgg aagaaagccc
      601 gcgaagaact ggcgcaggcg ggaggcattc ttctggcctg cggcggctcg gcggccgtct
      661 gcggtctgct ttacggcagc gtcttttcca gcgagcacat tctgcccgcc ctgtggtttc
      721 accccacggc ggagatcatg cgcctgtttt tcttcaccat catgatgggc gtggtgttta
      781 tccttgtcgg cctttgcctc aacatcatca acagtttttt gaatgcggat tattccgaag
      841 cctttctgga gaagcgggga ctggccatcc tcttcgttta cgcggctgtt gtcgtattcg
      901 tcctgctcta ccagaagacc ggcgagcccc ccgccttatg ggccctgggg gtttttatct
      961 tccttccgct gatgatcttt tcgctccgag gcctgctggg cgtgctgttt atggaaaaac
     1021 ccaaaccgca cagcatttcg gaatacatcg ttgaaacgat catggaggtg gtggagattg
     1081 cgctgggtct ttttgccaat acgatctctt tcattcgcgt gggcgccttt gctctttcgc
     1141 acgcgggact gagcattgtc acctatacgc tcgcggggat ggccgacccg tccctgaaat
     1201 ccgcgggcgc catcatcatc atcgtgtgcg gcaatatttt tatcatcggt ttcgaaggtc
     1261 tgatttgcgg cattcagtcc atgcgtctcg aatattacga gtttttcagc aagtttttca
     1321 gaggagacgg ggtggtcttt tccccgttta cactaccggc aaaagcatcg gaggtgtgag
     1381 atgaccagga gcaggcagtt ggcgtttctg atgggggcga ttttcctgtg cgccctgtgg
     1441 agtctctttc cggccatggg atgggcgtcc gagggagtcc cggccaaagc gtttacggac
     1501 gcaatcgggc tggcctacat cggcgcgggg gtggccgtgg ggtttgcctg tctggccagc
     1561 ggctacgcgg tggccaagat cggctccgcg gccatcggtg cggtgagcga aaaaccggag
     1621 ctgatggggc gcactctggt atttctgggg ttggcggaag gcattgccat ttacggcctg
     1681 attatcgcca tcatgattct gaataagctg taatatggaa aaaatttaca tgatcggaga
     1741 catggatacc gtc
//
LOCUS       DAOPAF010000133         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_3518
            NODE_61616_length_1598_cov_36.986390, whole genome shotgun
            sequence.
ACCESSION   DAOPAF010000133 DAOPAF010000000
VERSION     DAOPAF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865253
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:51:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,638
            CDSs (total)                      :: 1,610
            Genes (coding)                    :: 1,607
            CDSs (with protein)               :: 1,607
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61616_length_1598_cov_36.986390"
                     /isolate="AMR_MDS_3518"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PLQ77_08175"
     CDS             complement(<1..179)
                     /locus_tag="PLQ77_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPL10694.1"
                     /translation="MAKILVVDDEKDIADLVSYNLEREGFAVVKAFDGETALAKVRSE
                     KPDLLILDLMLPGVGG"
     gene            complement(270..530)
                     /locus_tag="PLQ77_08180"
     CDS             complement(270..530)
                     /locus_tag="PLQ77_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPL10695.1"
                     /translation="MAEKKIGEVLKFFAKPSVAAVHLSEGELQVGDSIKFSGHTTEFT
                     EVVQSMEVDNKPVQKAVAGDSIGLKVSDRVRPGDEVFKILPD"
     gene            complement(643..1047)
                     /gene="rplQ"
                     /locus_tag="PLQ77_08185"
     CDS             complement(643..1047)
                     /gene="rplQ"
                     /locus_tag="PLQ77_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPL10696.1"
                     /translation="MYHGKAGRKLGRSSSHREAMFRNMVTSIIRHESIRTTDATAKEV
                     RKLADRMITLGKRGDLHARRQALSVVRDKEMVAKLFGELAERFRNRAGGYTRIVKVGY
                     RFGDNAPVSILEYIPDENKKEKAKPKKAKKAE"
     gene            complement(1126..>1598)
                     /locus_tag="PLQ77_08190"
     CDS             complement(1126..>1598)
                     /locus_tag="PLQ77_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPL10697.1"
                     /translation="TINIDAIFSPIKKVNYVVSHARVGQITDYDKLVLEVWTDGSLNP
                     GDAIAYSAKILKQQLDVFINFEEVEEDAAPEQESPGGNVNEILMRSVEDLELSVRSAN
                     CLKNAGINTIGELVQKTEAEMLKTKNFGRKSLSEIKDILNEYGLAFGMKPDENA"
BASE COUNT          331 a          469 c          372 g          426 t
ORIGIN      
        1 ccgccgaccc cgggcagcat caaatccaga atcagcaaat ccggtttttc ggacctgact
       61 ttggcaagcg ccgtttcgcc gtcaaaggct tttaccacgg cgaatccctc tcgctccaga
      121 ttataggaca ccagatccgc gatgtctttt tcatcgtcaa caacgagaat tttggccatt
      181 tgcaaagact gtcacggcat gaatggacag caatccgcat cgcctgcatt aaaaataaac
      241 gccggcttcg attaaacgct taccgggggt tagtcgggaa gaattttaaa aacttcatct
      301 ccgggacgaa cacggtccga gactttcagg ccgatggaat cgcccgccac ggctttctgc
      361 accggcttgt tgtccacttc catggactgg actacttcgg taaattccgt cgtatggccg
      421 gaaaatttta tgctgtcgcc gacctgcaat tcgccctccg aaagatggac tgccgccacc
      481 gaaggcttcg caaaaaactt gagcacttcg ccgattttct tttctgccat ctcgattcct
      541 cctttgcagt agttgcccat aaataccttg atcgatttga aaaaacaacc acaaaaaaaa
      601 ggcagggcgc gcgccctgcc ttttatgatt cagttttatt ggctattcag ccttttttgc
      661 cttcttcggc tttgcctttt ctttcttgtt ctcatccgga atatattcca gaatggaaac
      721 cggcgcgttg tcgccaaagc ggtagccgac tttgacgatc cgggtgtagc cgcccgcccg
      781 attgcggaag cgctcggcca gctcgccgaa aagcttggcg accatctctt tatcccgaac
      841 caccgacagt gcctgccggc gcgcgtgcag atcgcctctt tttccgagcg taatcatacg
      901 atccgccagc ttgcgcactt ccttggccgt cgcatcggtt gtccggatgc tttcatgcct
      961 gatgatcgat gtcaccatat tgcgaaacat ggcttcccga tggctggacg atcgtcccag
     1021 ttttctgccc gccttaccgt gatacatggt tactttatcc tttcctgacc gtcccctgat
     1081 gaatccatca tcaaacgccg tcccgcgttc cggtcggttt cctttttaag cattttcatc
     1141 cggcttcatg ccgaaggcaa ggccgtattc gttgagaata tccttgattt cactcaggga
     1201 cttgcgtccg aaattcttgg ttttgagcat ttccgcctcg gtcttctgaa ccagctcccc
     1261 gatcgtgttg atccccgcgt ttttcaggca gttggccgaa cgcacggaga gttccaggtc
     1321 ttccaccgac cgcatgagga tttcattgac gttgccgccg ggagactcct gttccggagc
     1381 ggcatcttcc tcgacttcct caaaattgat gaacacatcc agctgctgct tcagaatctt
     1441 ggccgaatac gcgatggcat cgcccgggtt caggcttcca tccgtccaga cttccagcac
     1501 cagcttgtcg taatccgtga tctgtccgac gcgggcgtga gataccacgt agttgacttt
     1561 cttgatcggc gaaaaaatgg cgtcgatatt gatggtgc
//
